LOCUS       DAORTI010000001       116196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_39_length_116196_cov_7.635684, whole genome shotgun sequence.
ACCESSION   DAORTI010000001 DAORTI010000000
VERSION     DAORTI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 116196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 116196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116196
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39_length_116196_cov_7.635684"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLO70_00005"
     CDS             complement(<1..586)
                     /locus_tag="PLO70_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQZ72860.1"
                     /translation="MANVGKVKQVIGAVVDVQFDGKLPEIYNALELKRENGDTLVLEV
                     QQHLGEDSVRTIAMDGTEGLVRGTEVVDTGIAIAMPIGEGIKGRLFNVTGDPIDGLPA
                     VSKVGGRPIHNMPPMFENLSTATEVLFTGIKVIDLIEPYSKGGKIGLFGGAGVGKTVL
                     IQELINNIAKAYAGVSVFAGVGERTREGNDLMREM"
     gene            complement(741..1571)
                     /locus_tag="PLO70_00010"
     CDS             complement(741..1571)
                     /locus_tag="PLO70_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72861.1"
                     /translation="MSERKWNISLQQDAGRRRTELCKAQDFHTGWYKRWCAEMNEQPR
                     FHRKQWEFVYNMQALWERGCIGPGKKGLVFAVGTEPAPSIFANYGCEILATDIFPQKG
                     IEKGWTTDNQLCFGLESLNTRMLCEEAVFLEKVKYRAVDMNEIPDDLEEFDFNWSSCS
                     FEHLGTIEKGLNFLKNQLKTIKPGGWAVHTTEFNVSSNDETQDNNDTVIFRYRDIERL
                     IKELRESGHFVEDQDYSLGGMPEDFQVDTIPHQQDIHLKLQIDRFVVTSLGLIIQKKQ
                     "
     gene            complement(1585..2652)
                     /locus_tag="PLO70_00015"
     CDS             complement(1585..2652)
                     /locus_tag="PLO70_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQZ72862.1"
                     /translation="MKIILDCELMKHPNSGLYHYCLNLGLSVKSQLKEAGTGTISFYV
                     PPAEINSFGPDSNCIPEKKTFWNLFSPSSLSCDIWHAPFQSGRIVPDKKKFPGTKVLL
                     TIHDLNPLHEGKPIEEQKKSLSHTQSLIDKADALVCISQFCKEDVLQHCKVGNKPVYV
                     IRNGTHKVHTPELRSTSYHPVRPFLFGMGYVNRKKNYHVLLPLLENEEIELIIAGRLD
                     EQEYVEDIKKQAEKMGVIERLHLPGPVSEGEKAWYYQNCLAFVHPSLAEGFGAPVVEV
                     MQFGKPLFLSSLTSLPEIGGDVAFYFNSFEGKSMQQVFQKGMRDYNAIGLAERIKKRS
                     AEFNWEQKAKEYLAVYKSLAG"
     gene            2895..3953
                     /locus_tag="PLO70_00020"
     CDS             2895..3953
                     /locus_tag="PLO70_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQZ72863.1"
                     /translation="MHKIIFDCERMKYPDTGLYHYCLNLGKYLEKNINLREEQLHFYS
                     PPAQQDWSFTTSNHLTQSPFHKFKLPALNSYDIWHATYQNTHYMPMLNKKIKVVLSIH
                     DLNFMYDEKKSTAKKSKYLQYLQRLINRADVIVCISEYSKKDVLQHCDIKSKPLFVIH
                     NGSNLLQKPELTLGSYKPQKPFLFSIGVIHRKKNFHVLLPLLQQNTMELLIAGKHDDE
                     NYLSFIQNKSKELGVQDNVHLLGRVTENEKSWYFNNCSAFAFPSISEGFGLPVVEAMS
                     CGKPLFLSDRTALPEIGGDVSFYFHDFNAEHVRQVFHDGMELYNANGLRKKIKARSYN
                     FNWHKAAREYLEVYRSLY"
     gene            3977..5812
                     /locus_tag="PLO70_00025"
     CDS             3977..5812
                     /locus_tag="PLO70_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ72864.1"
                     /translation="MKVYKKLLAYARPYRWFVIPFFAFTILAVIFSVFQFALIIPLLN
                     VLFDNNSGKEILSPPTFSVSANFFKDIFYYQVYRLKAVNPVYALYFITFVIVCAVLLA
                     NLFRYLAQRTLIKARTLLVKRIREALFEKINHLHMGFFTKEHKGDLISRMNSDVFEIE
                     GVAANSLEVLFKEPSLIIGYFVALFAISTQLTLFTLIIIPISALGIATVQKRLRRDAK
                     DAQASIGRLLTIMDETLTGMRIIRAFNATGFTLKKFSKENDFYRNASLQGFKRREMAP
                     AFSEAAGVIVVAGILLYGGSLILQNGQTAAGIQASEFIAFIAIFSQVLRPAKAMVVAG
                     ANIQRGRAAGERILEIIDKPIEVKDKDDAALLNEFNYAIEFKNVDFSYNDMPVLKNVS
                     FTISKGKTVALVGVSGVGKSTIADLIPRFYDVKNGAVLVDGIDVRDYKMESLRSHMSF
                     VTQETFLFNDTIFNNIAIGKPDATEEEVMEAARIANAHDFIRQTENGYQTSIGDRGIR
                     LSGGQRQRLCIARAVFKNPSILILDEATSALDTESERIVQDALGKLMKGRTTLVIAHR
                     LSTIKEADEIIVLHEGQIIERGHHNELVEIEEGVYKKLTRMQQIA"
     gene            5851..7017
                     /locus_tag="PLO70_00030"
     CDS             5851..7017
                     /locus_tag="PLO70_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72865.1"
                     /translation="MQLTEVTTPQLSKEFIRVNVELYKNDPNYIRPLDKDINDVFDPE
                     KNKAFRFGEATRWILKDNTGKLAGRIAAFTNKKYKNKGDDVPVGGIGFFDCINDQLAA
                     DMLFDVAKHWLIQKGMQAMDGPINFGERDRWWGLLVEGFQPPPYCMNYNKSYYRELFE
                     TYGFKCFFNQICFGLDPRKEVSQKILDRHAQLAKDPAFSSRYIDKNQLEKFATDFAIV
                     YNKAWAGHGGLKELKKEQVYSMFKKMKPVMDEKIVWFVYHNDNPIAIFVNLPDLNQWF
                     KYLNGKFDLWHKLKFLWIKKTRKNIRFTGLVFGVIPEFHGTGVDAYMINEAKLVVHPM
                     NKYEEYEMQWIGDFNPKMINVAENFGDTFRTRKLTTYRYLFDRTKEFKPHPILS"
     gene            7082..8881
                     /locus_tag="PLO70_00035"
     CDS             7082..8881
                     /locus_tag="PLO70_00035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQZ72866.1"
                     /translation="MDKFIVSARKYRPQTFDTVVGQSHITTTLKNAIKHQQLAHAFLF
                     CGPRGVGKTTCARILAKTINCEKPTKEGEACDKCQSCTSFNDGASMNYFELDAASNNS
                     VDNIRDLVDQVRFVPQTGKYKVYVIDEVHMLSSSAFNAFLKTLEEPPPYAIFILATTE
                     KHKILPTILSRCQIFDFKRITTNDTVQHLEEICKKEEISAEKAALQIIAQKSEGCMRD
                     ALSILDKIVSFTNGELNYANTLEHLNILDADCYFKLLDCMQQQDLSGAMLLYDDINRK
                     GFEGDLVLNDFAEFIRNLLVCKDEKAAVLLEVVESFKDKYIATAKKVSLPLLISALNI
                     LSEAEINYKAARNKRLHVELTLIKLCYLQQAIEVTADATGISKKKIVEAPLAFRSVAI
                     KAFTGSTQVTEKAITRPNKESEARLIIQTPAVSEPLPDYQPASSQNPAAGNQQTKTGT
                     KISALDKIRRQYQGNGSNGNNNINHTLQEDSLKKAWSDYIARLKEARNPAAQPFELAV
                     LRIKDENTFEAITANNIEQKFIEQERNTLFHFLQQQLQNRLLQFSVIVEENADDRPKI
                     ELTLTAKEQFQKMADQYPLVKELKDRIRLELDY"
     gene            8955..10592
                     /locus_tag="PLO70_00040"
     CDS             8955..10592
                     /locus_tag="PLO70_00040"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HQZ72867.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKKVGDAVKKGDLLAEVETDKA
                     TMELESYKDGVLLHVGSDKGGKLQVNDLLAIIGTPGEDISSLIKQNSGNTVTGPEKKE
                     TTPVPAAVPAIVSPATIDIAKMDEVVLMPRLSDTMEEGVIAAWHKNVGDAVKKGEVLA
                     EIETDKATMELESYKNGTLLHIGAQKGEKIPVNGLLCIIGEKGKVDIDAIIAATKSGV
                     SPQPVAQSSKASSGPATASTTQIVTSASTENGRVKASPLAKKLAAEKGIDIKAISGSG
                     DGGRIIKADIDNYKTPATENVQPVTKVTAAASIPLAAFTGSTEEGYTDIPNSQMRNVI
                     AKRLGESKFSAPHFYLTMEINMDNAMAARTQLNEISPAKISFNDMVVKAVALALRQHP
                     AINASWMGDKIRRNHHIHIGIAVAIEDGLIVPVIRFADQKTLPQIASESKELAGKAKN
                     KKLQPNEFSGNTFTISNLGMMDIEEFTAIINPPDSCIMAVGRIKEIVVRKGEGFGVSN
                     VMKVTLSCDHRSVDGASGAAFLQTFKKYLENPITMLL"
     gene            10791..11012
                     /locus_tag="PLO70_00045"
     CDS             10791..11012
                     /locus_tag="PLO70_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72868.1"
                     /translation="MEEKEMKDFSWLTLFFVTSISSLLFSFVLKFLDGNHSTFLLWIG
                     AVTLAIGLIGWLGTALTKPPIRKKRNLAS"
     gene            complement(11026..12879)
                     /locus_tag="PLO70_00050"
     CDS             complement(11026..12879)
                     /locus_tag="PLO70_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQZ72869.1"
                     /translation="MKRIVLFLLLLLGFLLAEAQQKPFRFAFISDTHIGSPNGSAEED
                     LRRTIRDINAMKDVVFVVLTGDITELGTNEELKLAKKILDSLNIPWYIIPGNHDTGWS
                     ESGGVMFTTVFGYDKFSFEYNGMRFLGCASGPYVRMSDGHVPRDAVNWLDEELKKIKP
                     QQPVIFLNHYPIDNGLDNWYEVTDRLRNYNTWAILCGHGHSNRALNFEDIPGVMGRSN
                     LRAKANEGGYNLVDVRNDSILFTERKPVSAVQKTWTGVKAELKNYSTGKKFPRPDYSI
                     NEKYNQVKRIWENSSDANIISTPAVADEKVFVGNQNGLMTCFSIKDGKPLWKFKTAGA
                     VFSSPAVANGKIVFGSGDGNVYCLDLKKGKLEWKYKAGASVLGSPVINGDTIFIGGSD
                     HAFVALNSRNGKFIWKFSGLDGPVVSTPLLYDGKVIFGAWDRHLYALNSATGLLQWKW
                     NNGSAIRNYSPASCIPVAADGVVYIMAPDRFVTAIDVATGETLWRNKDGGVRESIGIS
                     EDGNWVYGKTMQDTIVAYAANRKKQSAAWKLHAGFGYEHVPSMLIEKDGFVFFGTKSG
                     VVYCIDPKEKKIIWMHKIDNSMVNTVRVLDKNQLVVSTMDGKLVLLKCISN"
     gene            12981..13487
                     /locus_tag="PLO70_00055"
     CDS             12981..13487
                     /locus_tag="PLO70_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQZ72870.1"
                     /translation="MKLTEAKQQFISSWGAFGTHWGINRTMAQIHALLLVSPDPLTQD
                     DIMEELNISRGNTNMNIRELINWGLVERVLLTGERKEYFTAEKDIWKVVKQIVKERKK
                     RELEPMLQLLDKLEEVEGDKRDKHVKTFVDTVSSIKKLGKQADKTLDTMIKAEENWFV
                     GNLVKLFK"
     gene            13964..14329
                     /locus_tag="PLO70_00060"
     CDS             13964..14329
                     /locus_tag="PLO70_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQZ72871.1"
                     /translation="MVKKKTLVLGASDNPSRYSYLAIQRLRNHGHPVTALGRKHTMVE
                     DVTIETGKKQFDNIDTVTLYLNPQHQQEYYEYILSLKPKRIIFNPGTENDELVNLAKK
                     KNIQVVEGCTLVMLSTNQY"
     gene            14528..15001
                     /locus_tag="PLO70_00065"
     CDS             14528..15001
                     /locus_tag="PLO70_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72872.1"
                     /translation="MKNIRTTVLCVCITLYNLSSFAQEQKIPINEPDYNKPKLFSHLP
                     DKIPVSIEKINEIITSPVGRTTGFRLSETSDFQFDGEVVSTASKYGNSIQSVVIRSGT
                     YEGARLTVSKITNADGSISYTGRIISFKHGDLYELQNQDGQLLLVKRNFYDLVNE"
     gene            15086..16684
                     /locus_tag="PLO70_00070"
     CDS             15086..16684
                     /locus_tag="PLO70_00070"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HQZ72873.1"
                     /translation="MKNFLQSVLTTLLLISVGNARAQVPVLSSHPSASAVLFLDFDGH
                     TVSGTSWNYAGPIVCAPSGLDNTKITTIFNRIAEDYRPFNINVTTDSTKYLAAPANKR
                     MRVILTVTHEWYGSAGGVAFVGSYSWGDDTPCFVFSGLLGYNVKNISEATAHEAGHTL
                     GLYHQASYDANCVKTSDYHYGQGAGEIGWAPIMGVGYYQNFTLWNNGPNSYGCTNYQN
                     DLSVITGSNGFSYRTDDHTANFAGATNAVFASDQFTVNGVIEQNTDQDLIKFIQPSFG
                     RFQLNAIPYNVGTGNAGSNLDLQVTLFNGAQTQLNIYNPGTLLSSVIDTNLNPGTYYL
                     RIEGKGNMYAPNYASLGSYALQGNFTAGNTLPLRQLLLQGQLNGDRHQLSWIIDADEA
                     VTEQVLEVSTDGRNFSPVTQSTTDARAFTYRPFAAGSLQYRLSVTFDNGRQYYSNIVT
                     LRNTGANPKPLLLSNLIGSNTITVTSPGTFDFAVYDFNGKTIAKGQLTNGINNINTNA
                     MTGGMYLIRFAKDGEQWTDKIIRQ"
     gene            16774..17247
                     /locus_tag="PLO70_00075"
     CDS             16774..17247
                     /locus_tag="PLO70_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HQZ72874.1"
                     /translation="MRWRKFNGDPIELPILDAVEHAIKRETEKGHRLKVCIGTDSQVK
                     GKETEYATVIVFLREGHGGFMFIHNEKTKQQFTIKERMLTEVAKSIEIAYELCNLFTI
                     YDVDMEVHADINTNPQFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKIVN"
     gene            complement(17306..17647)
                     /locus_tag="PLO70_00080"
     CDS             complement(17306..17647)
                     /locus_tag="PLO70_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72875.1"
                     /translation="MRKIVFAILLIVAIPVFSQTPGKKPSNKAGIKERVLLSDSVMYK
                     LKDFKDSIEKIQVNARINKVQVQTDKTADYSRVLQQEQNEEKKRGAIIRISIGLAMLA
                     LLIIGLRRRKK"
     gene            complement(17701..17895)
                     /locus_tag="PLO70_00085"
     CDS             complement(17701..17895)
                     /locus_tag="PLO70_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72876.1"
                     /translation="MKPILSERNIVVVLFVMVLIIFSLAQEDSKKMESMYAGAGNQVS
                     AKLVAEHTTLKRAELAPSAE"
     gene            complement(17957..18577)
                     /gene="yihA"
                     /locus_tag="PLO70_00090"
     CDS             complement(17957..18577)
                     /gene="yihA"
                     /locus_tag="PLO70_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQZ72877.1"
                     /translation="MEIISAKYVISSATHKQCPPADKPEYAFIGRSNVGKSSLINMIT
                     GQTKLAKTSATPGKTKLINHFEVISSDKKKWNIADLPGYGYAATVSQKARNNWGNMIE
                     SYIRKRENLVCLFVLIDSRHTPQAIDLEFINQLGEWQVPFVVVFTKTDKNKPGATVRN
                     VEAFIKSLSENWEEAPPYFVTSSTKKTGRKEMLSYIGGLNSSFKKL"
     gene            18719..19471
                     /gene="ubiE"
                     /locus_tag="PLO70_00095"
     CDS             18719..19471
                     /gene="ubiE"
                     /locus_tag="PLO70_00095"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQZ72878.1"
                     /translation="MSNILPHDNIIPFKDSEKSKKQQVADMFDKIASRYDLMNRFLSA
                     RTDIGWRKKAIQKLKKDNPQHILDVATGTADMALMAYKMLKPAYITGIDISEGMLELG
                     RKKIEKEQLGDKIQLHVGDSETIKSAENTFDAVMVAFGVRNFENLEKGLTEMLRVLKP
                     GGQLIVLEFSKPRSKAVKKFYNLYMGIVAPQVARWFKQNKEAYEYLNESAKAFPDRHL
                     FTDILKKVGYSNTEFQPLSFGICCIYSGRKPL"
     gene            19438..20187
                     /locus_tag="PLO70_00100"
     CDS             19438..20187
                     /locus_tag="PLO70_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ72879.1"
                     /translation="MLHLLRKKTFIKLITISPLVGGLLLFSFGASAQMGKVELNMYDH
                     DSKPYYFGITVGVNLARFQTDLHPRFLDYDSVFVAEPVNSGGLTLGLSATARLSDRFQ
                     LRFNPQLMFIERNIYYKLKHPDLDGLTDVTKKVESVIMSFPFQVKFQSDRIGNFRVYT
                     LAGFKADIDMASNARAKRAEELVKIQKYDFGPEFGIGFNFFFPSFIFSPEIKISNGIR
                     NIHARDENLRFSNVFDKIQSRMIVFSIHLEG"
     gene            20564..20836
                     /locus_tag="PLO70_00105"
     CDS             20564..20836
                     /locus_tag="PLO70_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HQZ72880.1"
                     /translation="MPKYVIEREIPNAGKLNAEQLKSISQTSCGVLSKMGSAIQWVQS
                     YVTGDKIYCIYIAPNKEMVLEHAKQGGFPANEVNEVMTIIDPTTAD"
     gene            complement(20948..22273)
                     /locus_tag="PLO70_00110"
     CDS             complement(20948..22273)
                     /locus_tag="PLO70_00110"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HQZ72881.1"
                     /translation="MGIIKDRFKAKADAVAIEIKDLLKEHGNKVIGEVQLSQIYQGMR
                     GITGLVTETSLLDAQEGIRFRGYSIPELQQKLPKAPGGSEPLPEGLFYLMMIGELPTE
                     EEANHVTNILQRRSHVPTHVFEAIDALPVSAHPMTMFVVAVMALQTESYFQKKYAEGL
                     SKKDYWLPVFDDSLVLLSRLPRIAAYIYRRKYKNSEHIQPNGLLDWAGNFAHMLGFED
                     ESFKELMRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQ
                     EVIKWIFEMQEELKTDLPSKEQIADYVKKTLSEGKVVPGYGHAVLRKTDPRFTAQMEF
                     GKKHMPEDKLVQTVWNVYETVPPILQTIGKIKNPWPNVDAHSGALLVHYGMVEYEFYT
                     VLFAVSRALGVLASLCWDRALGFPLERPKSVTTDLVKQWLDGKDNIWGD"
     gene            complement(22401..23498)
                     /locus_tag="PLO70_00115"
     CDS             complement(22401..23498)
                     /locus_tag="PLO70_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQZ72882.1"
                     /translation="MQKLDWYILKKFLVTFIFCMLLFTVIAVAVDSSEKTDDFVKADF
                     TTSEIINKYYLGFVPYIWSLLFPLFVFIAVIFFTSRMATRSEIIAILASGTSYNRFLR
                     PYVIGGVLLAILLWVGSRYWIPKANIIRSDFQTTHIDKNDPTKNRAFGSCARCFYLRT
                     DTNTFIGIRDYDTTSKTARGFFMEKIKDNKVVYNLRAETLQWDTSIRKWKLFRVAERY
                     VDSIGERLKNIDTLLINLNLNPAELRKDDYLKDKLTTPELVRFIEKEELRGTEGLNTL
                     KVERYRRSATPAAVLLLTMIGVVIASRKTRGGSGMHMALGIIIAALFIMSDRFSTVFA
                     TKGNFPPLLAAWLPNIVFAGVAFWLYRRTPK"
     gene            complement(23580..24719)
                     /gene="tgt"
                     /locus_tag="PLO70_00120"
     CDS             complement(23580..24719)
                     /gene="tgt"
                     /locus_tag="PLO70_00120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQZ72883.1"
                     /translation="MPALQFQLQHKDSSSKARAGKITTDHGEIMTPIFMPVGTVGSVK
                     AVTQQQLVNDVKAQIILGNTYHLYLRPGLEVLEAAGGLHQFNGWNKPILTDSGGYQVF
                     SLAGTRKIKEEGVTFQSHIDGSKHLFTPENVMDIQRSIGGDIIMAFDECPPGGSDFSY
                     AKKSMEMTHRWLDRCFQQFNATQPKYGYMQNLFPIVQGGTFKDLRKQSCEYISSKEAN
                     GNAIGGLSVGEPEPVMYEICDWCCDHLPVDKPRYLMGVGTPWNILECIGMGVDMFDCV
                     MPTRNGRNAMLFTSKGVINIDNKKWENDFSPIDDNFDCEVSNFYSKAYLRHLMKAKEF
                     LGYTIASIHNLAFYLWLVGEARKHIQQGNFNIWKNEMVERLKQKL"
     gene            24809..25927
                     /locus_tag="PLO70_00125"
     CDS             24809..25927
                     /locus_tag="PLO70_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ72884.1"
                     /translation="MPPINWGEILLYFFIAVTAIQLFYYGWFFSRVAFFKPKAKVQSQ
                     QHPVSVIICSRDEDENLARNLPGVLVQQYSSSHEVVVVNDNSLDDSKYILQELKKTFK
                     SLHVVELTQEAVHISGKKYPLSIGIREAKYEVLLLTDADCVPASEHWIQKMQDGYDKN
                     IEIVLGYGAYHKRKGLLNKLIRFETFHTALQYLSYALAGIPYMGVGRNLSYKKDLFLR
                     SKGFSSINHIPSGDDDLFINKVATKNNTAVVIDPDAITRSIPKTTWGSWLRQKSRHYT
                     TAKFYKPKHKFLLGLYFATQFIYYPLLVTTAILYDWRFALAIFGLRLALQAFVLYKSM
                     NKMGEKDLWPWFIFLDMWMFFYYIIFAPALWRRPRNKW"
     gene            25960..26592
                     /gene="rsmG"
                     /locus_tag="PLO70_00130"
     CDS             25960..26592
                     /gene="rsmG"
                     /locus_tag="PLO70_00130"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQZ72885.1"
                     /translation="MEIILKYFSDFTEIQLQQFRKLEGLYKEWNEKINVISRKDIDSL
                     YEKHVLHSLSIAAAFEFEPGSEIIDIGTGGGFPGIPLAIFFPELKFHLADSIAKKLKV
                     VEAVAGSIGLKNVTTQHTRAEEIKNRKFDYAVSRAVAPLKDLWTWSRPLLHNKPRTVD
                     TIIKPGLICLKGGDLAKEIQESGTRPHMIEIKDLFPEDSFKEKYLLYVPR"
     gene            26647..27294
                     /locus_tag="PLO70_00135"
     CDS             26647..27294
                     /locus_tag="PLO70_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ72886.1"
                     /translation="MRQISVFIVDDNKELRNALEEIVSMSEGYKCIGTVGSAEEAVIQ
                     IPILKPDVVLMDINLGTEESGIDVVRVLKPRIPDTNFMICTVYEEDEKIFEALSAGAS
                     GYILKKTSPGKMLEAIRELYEGGAPMSSQIARKVVAAFQQQQNQFAVNDSEQLNLLTN
                     REMEILEFLSKGLMYKEIAFQTFLSPETVRKHVYHIYEKLHVTNRVAAVNKFYGR"
     gene            complement(27525..29366)
                     /locus_tag="PLO70_00140"
     CDS             complement(27525..29366)
                     /locus_tag="PLO70_00140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ72887.1"
                     /translation="MNKLFVLSVGSFFVLLKADAQPDRWQQKVKYTMSINMNVQTNQF
                     AGKQKLEYWNNSPDTLTRVFYHLYFNAFQPGSMMDTRSRRQGTIIAGRGPDWDQRVKD
                     RIQNLKPEEIGFQKIISFKMNGRPQKFNILETILEVKLDKPILPKSKVVFDMEFEAQV
                     PLQIRRSGRDNPTSKVRYSMSQWYPKLCEYDYEGWHPTPYVGREFYGVWGDYDVSISI
                     DKKYILGGTGYLQNPNQIGYGYESAGVKVSRPAGDKLTWRFVAPNVHDFMWAADPEYV
                     HKTRKVNDTVTLHLLYKSTNATPAEWEKILEDAQKALPFIEKKFGVYPYRQYTFIHGG
                     DGGMEYPMATLLIGPGAWLHEWLHNWYHGLLGNNESLYGWMDEGFTTYAEDLVTAFLE
                     NSTAFPFDGTYRGYYALVKSGREEPLTTHADHFNTNAAYGAAVYSKGAVFLEQLGYIT
                     GAPVRDKILLEYYRQWRFRHPNAADFIRIAETVSDMKLDWYKEYWVNSVKTIDYSIDS
                     LWEEGGKTKVRLNKVGLMPMPIDLQFTFKDGSKELHYIPMNLMYGEKPVEDSAISRKV
                     YEAWKWTHATYTVETTRKLADFSIVEIDPTMRMADIDRKNNKLDLKW"
     gene            29408..30091
                     /locus_tag="PLO70_00145"
     CDS             29408..30091
                     /locus_tag="PLO70_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQZ72888.1"
                     /translation="MIKTKLINGYPFMAYEKESFLPAEMTKRSLEFYKWMDKRRTVRD
                     FSDKPVPKETIENILMAASTAPSGAHKQPWTFCVVSDPGLKKQLREEAEKEESESYNG
                     RMPQEWLNDLLPLQTDWRKEFLETAPWLIIVFKKSYELNSDGSKGNVYYASESCGLAC
                     GFLLTAIHHAGLVALTHTPSPMNFLSKLLNRPENEKPFLLIPVGYPADECWVPVLKRK
                     ELNEMSVWL"
     gene            complement(30088..30753)
                     /gene="rsmI"
                     /locus_tag="PLO70_00150"
     CDS             complement(30088..30753)
                     /gene="rsmI"
                     /locus_tag="PLO70_00150"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQZ72889.1"
                     /translation="MLYLVPTPIGNLKDITLRAIEVLKEVDLILAEDTRTTSHLLTHY
                     QITKPLSPYHQHNEHKVLHHLVSQLLEGKKMAVVTDAGTPGISDPAFLLVRECIKVGV
                     KVECLPGATAFVPALVNSGIPSNRFSFEGFLPLKKGRQSLLKQLAEEERTLIFYESPL
                     RLVKTLEEFITYFGESRQCSVSRELTKMFEENKRGSLREVADYFNGKQVKGEIVIVIA
                     GKD"
     gene            complement(30841..31086)
                     /gene="purS"
                     /locus_tag="PLO70_00155"
     CDS             complement(30841..31086)
                     /gene="purS"
                     /locus_tag="PLO70_00155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQZ72890.1"
                     /translation="MTYTVQIKVMPLKELLDPQGKAVMGGLENLGLGGVEDVRVGKNI
                     TLQVNAESPEKARAIAEEASKKLLSNPVMEYFEVSVN"
     gene            complement(31083..31847)
                     /gene="pssA"
                     /locus_tag="PLO70_00160"
     CDS             complement(31083..31847)
                     /gene="pssA"
                     /locus_tag="PLO70_00160"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQZ72891.1"
                     /translation="MKQIPNLFTLLNLFFGCIAIVFTLQNGILINVSAEGTELLDIPE
                     KIWMTSLFIGLAAIVDFLDGFVARLFKATSEFGKQLDSLADVVSFGVAPAMIIYQFLR
                     LSFAQEEGGVEISLLWLIPVFILPCAAAWRLARFNLDTSQSYSFKGMPVPAVGLFVAS
                     LPLIYWNVNEPWVYNLLLNKWFLYGNVLVLSYLMISTIPLMAMKFKDYSWQNNLPRYL
                     LIVIAVLAIVLLKWLSVPVIVLAYVLLSLLFKNKLA"
     gene            complement(31863..33152)
                     /locus_tag="PLO70_00165"
     CDS             complement(31863..33152)
                     /locus_tag="PLO70_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HQZ72892.1"
                     /translation="MKYLPLNPEIFVQNRERFARQMQKNSIAIFVSNDEVPSNGDAIF
                     SYKQNSDLFWLSGITQEDSMIILFPDNPDPKYREVLVLVRPNELKEKWDGKRLRVKEA
                     QDISGIKTIVWLDVADGLLQPWIHLADNIYLDSNENDRKASLVRSRDYRFIDEMKSRY
                     PLHNYLRAAKIMKELRGIKTALEVEVMQKAIDITENTFRRLLKFIKPGVMEYEIEAEI
                     FHSFLSQRATGEAYGSIIASGDRARTLHYVSNNAECKDGELILMDFGAEYGGYCADLT
                     RTVPVNGKFTRRQKTVYNSCLHLHNYAKSILKPGISILEYTDKVGEEATQQFLKIGLL
                     RKSDVKNEDPENRAYRKYLYHGISHHLGVDVHDLGTRTEPIKAGMVFTIEPGIYIEEE
                     QMGIRIENNFWITRNGNKDLMKNIPITAEEIESLMKK"
     gene            33452..35056
                     /gene="pckA"
                     /locus_tag="PLO70_00170"
     CDS             33452..35056
                     /gene="pckA"
                     /locus_tag="PLO70_00170"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQZ72893.1"
                     /translation="MSVPTIPFPTQNLFKSSLKNTESIHYQSSPEELVQDTLRIGEGV
                     LNDTGALVIKTGEFTGRSPRDKFTVKDEITTDTVHWNEFNIPMETKNFDIIFEKVSAY
                     LNSLNEIWVRDCYACADPRYRLNIRVINEKPWNNLFAYTMFLRPTEKELESFEPDWHV
                     ISVPGLRLDPAACGTRQHNASVVSFKHKMILIAGSGYTGETKKGIFTILNYILPQEKN
                     VLSMHCSANMGTDGDTAIFFGLSGTGKTTLSADPNRKLIGDDEHAWTDDNIFNFEGGC
                     YAKTIHLTEEKEPEIYNAIRPGALVENVTFYPGTNKINFSDASVTENTRVSYPLHFIS
                     NAQEPSIGNLPKNIFFLTCDAFGVLPPISKLTPGQAMYQFISGYTAKVAGTEAGITEP
                     KSTFSACFGAPFLPLHPGKYAAMLGKKMQENKVNVWMINTGWTGGPYGVGSRMKLKYT
                     RAMITAALEGKLNETEFDTDPIFGMAIPKQCPDVPSEVLHPRNTWVDKNAYDEKAKYL
                     AGLFIKNFTKYADGVTPEILAAAPKA"
     gene            35183..35728
                     /locus_tag="PLO70_00175"
     CDS             35183..35728
                     /locus_tag="PLO70_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HQZ72894.1"
                     /translation="MQNFLKYFLLFLLPITASAQLPDEELLEWSSERKLSWSDYKGKP
                     NPLSDAAASTTTYLSIEYNISSSSFGYKIKSRFSKNRSWGLHKSDYILSHEQGHFDIA
                     EIFARKLHKRMGEYSFNKRTYQQDLKKIYEEVTDEKEQMQNDYDRETRHSINKEKQAE
                     WLKKIERMLEDLKVYHNYKSL"
     gene            complement(35736..36371)
                     /locus_tag="PLO70_00180"
     CDS             complement(35736..36371)
                     /locus_tag="PLO70_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQZ72895.1"
                     /translation="MAKAKKPKHIAVAGNIGAGKTTLTEMLSKHYKWIPHFEDVDHNP
                     YLMDFYEDMPRWSFNLQIFFLNSRLRQLVEIQKGTETVIQDRTIYEDANIFAPNLHEM
                     GLMSKRDFDNYFHFFQTLKTLVQPPDLLIYLNASVPTLVGQIQKRGREYEENIRLDYL
                     KKLNEFYNKWIGEYKEGKLLVIDVDKNKFAERDEDFGEIIRKVDAELYGLF"
     gene            complement(36473..37477)
                     /gene="trpS"
                     /locus_tag="PLO70_00185"
     CDS             complement(36473..37477)
                     /gene="trpS"
                     /locus_tag="PLO70_00185"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQZ72896.1"
                     /translation="MEKRKEIVMSGIRPTGFLHLGNYFGAVRNYVRMQEDFECYFMVA
                     DLHSLTTHPDTKELKANVHRVLAENIACGLDPDKVALYCQSHIYETAELYLYLNMLAY
                     KGELEKTVTFKDKVRQHPDNVNAGLLTYPVLQTADIILHRALYVPVGKDQEQHLEMAR
                     NFANRFNHRYGEVFPEPQAFNYGSELVKVPSLDGAGKMSKSENQYATLYLSDDDELIR
                     KKVMKAKTDSGPTVPGSPKPDYIENIFLLMNLVSSEEVIKKFEEDYNQCTIRYGDMKK
                     QLAEDMVNFISPIREKAESIRNDERYLQEVMEKGAAKARKSAKATMELVREAIGLKYF
                     "
     gene            37586..37876
                     /gene="gatC"
                     /locus_tag="PLO70_00190"
     CDS             37586..37876
                     /gene="gatC"
                     /locus_tag="PLO70_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQZ72897.1"
                     /translation="MEVNDKLVDKLAHLSRLQFNESEKAEIKNDLQRMIGFVEKLNEL
                     KLDGIEPLLHMSEEVNVLREDEIKGSVSREEALKNAPLHDEQFFKVPKVIKK"
     gene            37878..38591
                     /locus_tag="PLO70_00195"
     CDS             37878..38591
                     /locus_tag="PLO70_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ72898.1"
                     /translation="MAQGIIHLENIQKSYFMGKNELQVLKGVSLDIFKNEYVALMGPS
                     GSGKSTLMNIIGCLDTPTAGRYILNDNDVSEMEDNALAEVRNKEIGFVFQQFNLLPRL
                     TAAENVALPLVYAGMNRKLRTEKAMQVLEMVNLTDRSHHKPNELSGGQCQRVAIARAL
                     VNNPSIILADEPTGNLDTKTSYEIMEIFNTIHTGGNTIVLVTHEEDIANHAHRIVRLR
                     DGIIESDKKNENVGEYSHH"
     gene            38674..39234
                     /locus_tag="PLO70_00200"
     CDS             38674..39234
                     /locus_tag="PLO70_00200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQZ72899.1"
                     /translation="MTFKIYTKTGDKGTTSLIGGTKVPKSHLRIEAYGTVDELNSYIG
                     LCRDILTDENSRTVLQEVQDRLFTIGSSLACDPIKEPKMRIPDLKEKDVELLEKEMDK
                     LNESIPEMKSFILPGGHTTVSHLHIARCICRRAERCCVRLELESLEVEQVILKYLNRL
                     SDYLFVLSRYTGHQLLVGEIPWKPRV"
     gene            39358..40233
                     /locus_tag="PLO70_00205"
     CDS             39358..40233
                     /locus_tag="PLO70_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ72900.1"
                     /translation="MRLQLISAVTSLTYLLSSCQPSPVDPPPNGPVVLRDTTINNILY
                     GADPAQRLDLGLPANRTSNTQLVVIIHGGGWATGDKNELTWALNGLKQRGFAVANINY
                     RLTLNTADNYKMQLDDIDSAVQFTLRQSSVHTFNGQKLYIVGHSAGGHLSLCYAYTRN
                     TNGKVKAAGSLAGPTDLFAMAYYSFNIYNPLLNPYLGMPLIPVTPVSEQRYKSCSPQY
                     QATTTAPATIFLHGELDIIVNPDQSSGMYAKLGTLGVDRKLVLYPLTFHDWWTDGTKV
                     ANTLDELKTWFNNHP"
     gene            complement(40245..40898)
                     /locus_tag="PLO70_00210"
     CDS             complement(40245..40898)
                     /locus_tag="PLO70_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ72901.1"
                     /translation="MITGKKIYKRYGKVEVLKGVDVEIGRGEVVAIVGPSGSGKSTLL
                     HILGTLDKADMGEVSMNNVIINSLSGKKLAAFRNKHIGFVFQFHHLLPEFSALENVSI
                     PAWLAGRKKPEVKTEAEKLLTMLGLSQRMDNKPNQLSGGEQQRVAVARALINKPDIIF
                     ADEPTGNLDSANAKDLHRLFFDLREQFSQTFLIVTHNEELAQLSDRVLHMKDGKIVQ"
     gene            41101..41322
                     /locus_tag="PLO70_00215"
     CDS             41101..41322
                     /locus_tag="PLO70_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HQZ72902.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYSIRSGAPIEVVENLQELE
                     DEGDVYETIEDIWPDYPTQDDFLFNEDEY"
     gene            complement(41488..42306)
                     /locus_tag="PLO70_00220"
     CDS             complement(41488..42306)
                     /locus_tag="PLO70_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ72903.1"
                     /translation="MAYNLLKGKRGIITGALDENSIAWKVAEKAYEEGASIVLTNAPI
                     AMRMGEINKLAEKTRSEIIPADATNVDELTTLFTKSQEVLGGKIDFVLHSIGMSVNIR
                     KKIPYTESNYDYFMKGIDVSAMSFHKMLAVAKKLDAINEWGSVVALTYMAAQRTYPFY
                     TDMADIKAMLESIARSFGYHYGVDKKVRINTVSQSPTKTTAGTGIKGFGDFFDFANSI
                     APLGNATAEDCANYCITLFSDLTRMVTMQNLFHDGGYSTTGVSNEVMQKLGVSE"
     assembly_gap    42366..42465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            42875..43951
                     /locus_tag="PLO70_00225"
     CDS             42875..43951
                     /locus_tag="PLO70_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQZ72904.1"
                     /translation="MDIRTIITGTGCYIPPITKTNKEFVTQDFYGEDGKLITTPTQDI
                     VEKFGEITGIEERRYAAEDMNSSDMATIAARLAIEDSGINPEDLDQIIVAQNFGNVIK
                     HTIQTDVLPALASRVKHDLGIKNPNCVAYDILFGCPGWVQGVIQADTYFKAGVAKKAL
                     VVGTETLSRVIDIYDRDSMIFSDGAGATVLEARPAGSTNSGILSWSVQSHCLDEAYYL
                     FLGKSNLPGSDPRIRYIKMKGRKVYEYAMLHVPAAMKDCLDKAGISIQEVKKVFIHQA
                     NEKMDEGIIRRLYKLYGIREIPEHVMPMSIHKLGNSSVATIPTLFDMVKKGFWDNHDL
                     REGDIVLFASVGAGMNINAICYRM"
     gene            44018..46444
                     /gene="pheT"
                     /locus_tag="PLO70_00230"
     CDS             44018..46444
                     /gene="pheT"
                     /locus_tag="PLO70_00230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQZ72905.1"
                     /translation="MTISYKWLSEYLPVTVDPERLSRILTSIGLEVESMNKFEEVKGG
                     LKGLVIGEVVSTEKHPNADKLTITNVNIGRTDPLQIVCGAPNVAAGQKVVVATAGTTI
                     YPSTGDPLTMKVATIRNVESHGMICAEDEIGLGTSHAGIMVLPGEVTVGTPAAEYFQP
                     YNDIVYEIGLTPNRMDAMSHWGVARDVCAYLSHHDKKDIKPKLPITNSFKIDNTSFLI
                     DVKVENATACPRYSGISIDNVTIKESPNWLQQKLKAIGLRPINNIVDITNYIQHETGQ
                     PLHAFDADAIYGRKVIVKNLPAGTRFVTLDEKERILSAEDLMICDEKEGMCIAGVFGG
                     LHSGVTEKTKTIFLESAFFDGITLRKTSFRHGLRTDAASRFEKGTDISATVNVLKRAA
                     NLIKEICNGEITSEIVDIYPKKKEKTEIAIKWHYIKKLSGKNYHPDAVKDILNSLGFE
                     IMKEGIDELRVAVPYHKPDITLPADIVEEIVRIDGLDNIDIPEAITITPAVEENYAKE
                     VYREKTANYLVGLGFNEMMTNSITNAAYFSEEELQTMVTMKNSLSAELNILRNSLFET
                     ALEVVAHNLNHKNNSLRLFEFGKAYSSSGPGRYNESEKLCIVISGNKNEDSWKQKSVS
                     ADFYLLKGTVDATLQSLGVLPVSTEKLLAPKLDNHIVYKYNNQIIAGAGEVKKTILDK
                     FGIKQPVFLAELNWQFLSEQASTQTTTIKEISRYPAVQRDIAMIVSKELVWEEVHMTV
                     QKIKLDKLQDIKLFDIFESEKLGAGKKSMAVNFTFQDEGKTLTDKEIDGWMSKIMTSL
                     EKELQAEIRK"
     gene            46441..46728
                     /locus_tag="PLO70_00235"
     CDS             46441..46728
                     /locus_tag="PLO70_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72906.1"
                     /translation="MSNTEHHLKRIQDKLQQLLKQYAALQKENSRLIQELASAEQKIE
                     AYQKNTDELKQQVSVLKLSTGEMSEADKKEFEKRINGYLKEIDRCIALLGE"
     gene            complement(46730..46984)
                     /locus_tag="PLO70_00240"
     CDS             complement(46730..46984)
                     /locus_tag="PLO70_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72907.1"
                     /translation="MFAKVKSSVFIDVLQFQLNTPKGNCLRGVLKDDTGSICRTMEKN
                     IAKDREELIWGGLNDLPYGRYTLELSQGENEMKMNLVKRV"
     gene            47113..47403
                     /locus_tag="PLO70_00245"
     CDS             47113..47403
                     /locus_tag="PLO70_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQZ72908.1"
                     /translation="MNELIPISALIGDRTYRIKIDPKDEEVVRNTLKTINDKIIEFKT
                     LFAGKDMQDYIAMVMIWYATEQQAGKSALLEKVNLGEKLDSIEKLLDNHINQ"
     gene            complement(47468..48640)
                     /locus_tag="PLO70_00250"
     CDS             complement(47468..48640)
                     /locus_tag="PLO70_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ72909.1"
                     /translation="MKKIILLIGLLPFFALAQDKSKPFSLAGKVKNLKYIPDWVFLQY
                     RTNGEWKTDSLQTKDGKFSFAGEIAEPVLGRLRVKYRSGDDGNKIATVSGRDIVAVFL
                     EPGKIKITSVDSFSNVKVKGSAAHSEFVKLKEQEKPFEERMKPLIAKYTEYGKAKDRE
                     NQSKVEDQIDAIDKEKSEKVYGEYVKKNPSSPLAMYALQQYAGWDIDADKVEPVFNSL
                     SGTNKNYPSAIEFKENLEIAKKTGIGKMAMDFTQNDTLGNPVSLASFKGRYLLIDFWA
                     SWCGPCRTENPNVVKVFNKYKDKNFHIIGVSLDRPGQKEKWMKAIHDDKLDWTQVSDL
                     KFWDNEVAKQYGIKAIPQNLLLDPEGKIIAKNIRGEELETKVGEAIEGKKGFVPVP"
     gene            48868..50433
                     /gene="rny"
                     /locus_tag="PLO70_00255"
     CDS             48868..50433
                     /gene="rny"
                     /locus_tag="PLO70_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQZ72910.1"
                     /translation="MDPNILSVIIGAAALIVGIILGKFLFAKNTRKQIEEAELQSQTI
                     LKEAELRAETIRKEKELEAKEKFVQMKSAHDREILDKNKKLNDTENRIRQKEQTINQK
                     EGNLDKQIKENEAIKENLNRQIELVNIKRTELEKHQEEHIRRLEKISNLSAEDAKAQL
                     IESLKNEAHSKALALQQEIIEDAKQKAGKEARKIVIQTIQRTAAEQAIENSITVFNLE
                     SDEIKGQIIGREGRNIRAIEAATGVDLIVDDTPEAILLSSFDPLRREIARLSLQRLVA
                     DGRIHPARIEEVVEKTKKQIEEQVMEIGERTVIELGIHGLHKELVRMVGRMRFRSSYG
                     QNLLMHSRETANLCATMAAELGLNAKLAKRAGLLHDIGKVPDEESELSHALLGAKLAE
                     KYGENPAVVNAIGAHHDEMEMQFVISPIVQACDAISGARPGARREIMQQYLQRIKDLE
                     NLAMAYNGVEKAYAIQAGRELRVIVEADKVTDQESDKLSFEIAQKIQTEMTFPGQIKV
                     TVIREKRAVNVAR"
     gene            50569..50937
                     /gene="rplS"
                     /locus_tag="PLO70_00260"
     CDS             50569..50937
                     /gene="rplS"
                     /locus_tag="PLO70_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQZ72911.1"
                     /translation="MNAIAYVHEQLSGKKEFPAFKAGDNITVNYRIIEGNKERIQSFK
                     GDVIKKQGNGATASFTVRKISDGVGVERVFPFFSPNIESIEVNKTGHIRRAKLFYQRT
                     RSGKSARIREKRQAVAEVVK"
     gene            51061..51270
                     /gene="tatA"
                     /locus_tag="PLO70_00265"
     CDS             51061..51270
                     /gene="tatA"
                     /locus_tag="PLO70_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQZ72912.1"
                     /translation="MIQPTLLGMLGTNEIIIILVIVLLLFGGRKIPELMRGLGKGVRE
                     FNDAKSNVKKEIEESASDVKNSVKD"
     gene            51317..52741
                     /gene="gatA"
                     /locus_tag="PLO70_00270"
     CDS             51317..52741
                     /gene="gatA"
                     /locus_tag="PLO70_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQZ72913.1"
                     /translation="MFEFTSIEQFHAQLQEGKVTCLQVVQYYLKQIHSTRHLNAFIEV
                     YADEALQRAKLLDEKRLAGQTIGKLHGVVIGIKDVICYKDHKVSASSNILKDFTSIYS
                     ATAVERLLSEEAIIIGNLNCDEFAMGSTNENSSYGNVLNALDETRVPGGSSGGSAVAV
                     QAGLCMLSLGSDTGGSVRQPADFCGIIGLKPTYGRISRYGLIAYASSFDQIGIFGNNV
                     PDVAVLLEVMAGADEYDSTVSHREVPAYSSQLPGNKKFRFAYFDEALNHPSLDIEIAN
                     SIKNKIEQLKEDGHEVAAIEFDLLDYIVPAYYVLTTAEASSNLSRYDGIKYGHRTAKK
                     DIDLTEFYKLTRSEAFGKEVKRRIMLGTFVLSAGYYDAYFTRAQKVRQLLCDKTAAIF
                     KFYDFIIMPTSPVTAFKAGEKMDDPIAMYLADIYTVMANLVGCPAISIPLFKHSNGMP
                     FGLQVMANRFDEVSLLQISSMLKP"
     gene            52794..53858
                     /locus_tag="PLO70_00275"
     CDS             52794..53858
                     /locus_tag="PLO70_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQZ72914.1"
                     /translation="MTFLKRILPFTIGLLLLLSARAGKNQQAPKNEFSGIINKDVNKD
                     SLIQPTSILKDPKLGFKNLFETKTLGGVTGTRLNPMAVSFVESYMAKYGKGLRGMRNW
                     AKPYFDMIDNVLTQHGLPKELKYLAVIESGLRYNAISWAGAVGPWAFMPAAGRQYGLI
                     VTNGYDERLDYYKSTHAAARMLTDLYLKYQDWLLVIAAYNCGPGNVDKAIRRSGSKDF
                     WKLQYHLPTESMNHVKKFISTHYVFEGEGGITTVTKEETKELLLNYPSTLKEEEFKTS
                     TAYKITGRFNSSVIVKYTGIDQLTFNRYNPGFNDTIALNGTYELRLPTEKMNIFIAKR
                     YQILEESMQLLLNPAGSNSR"
     gene            complement(53872..55113)
                     /locus_tag="PLO70_00280"
     CDS             complement(53872..55113)
                     /locus_tag="PLO70_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQZ72915.1"
                     /translation="MKQKVLNWAKRFNTFCFLDNHQYQHEPHTVECLLAAGVKRQLTA
                     NTGDALEQLQTFLDAGNSWLFGHLGYDLKNEIEELSSSNPDKIQFPDLFFFEPEVVIW
                     LSATELVIESDNPHLVYKGITEVCAATDEVGPYSFTIQSLISQKEYISIIKKLKQHIL
                     RGDCYEINFCQEFFAENAVIDPFSVYKKLSKVSPNPFSALYKLNDKWLICASPERFLK
                     RKGPQILSQPIKGTAKRILNSAAEDQKSKVGLLNSAKDRSENVMVVDLVRNDLAKICK
                     EGTVKVDELYGIYSFPQVHQMISTVSGILKEEVPFSEIIRATFPMGSMTGAPKKRVMQ
                     LIEQYERTKRGIFSGAIGYISPDGDFDFNVVIRSIMYNASSGYLSFEAGSGITYYSDP
                     ELEWEECVLKAEAIKYVLEKI"
     gene            complement(55191..55631)
                     /locus_tag="PLO70_00285"
     CDS             complement(55191..55631)
                     /locus_tag="PLO70_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72916.1"
                     /translation="MATKPVKEKKTTGKANAASEKEAAKKSSPKPSKKEEEEDDDDDI
                     DDDEEVKPSKKGGKAAASKKSGDEDDEEDAEDEPDDWEKPEEEEEWDPDFDEFDIPKS
                     KGKKAGGAAPAGKKAAEEDDFKFEDDEFKDLFDDKDYDDDDDDY"
     gene            complement(55756..57021)
                     /locus_tag="PLO70_00290"
     CDS             complement(55756..57021)
                     /locus_tag="PLO70_00290"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQZ72917.1"
                     /translation="MVDVILGLQWGDEGKGKIVDFFAKDYDLVARFQGGPNAGHTLYV
                     NDKKVVLHQIPSGVFHEGIINLIGNGVVLDPVTLKRECDTVASFGIDLKKNLFIAERT
                     HLILPTHRALDKAAELSKGNQKIGSTLKGIGPTYMDKTGRNGLRVGDLLDKNFTTQYI
                     KLRLKHQRLLDNYNFQEDITAWEEEFFETVEFLKQLNIVNGEYFINNQIKAGKRILAE
                     GAQGSMLDIDFGTFPYVTSSSTISAGVCNGLGIAPQQIRDVIGVTKAYCTRVGGGPFP
                     TELENETGEELRKIGNEFGATTGRPRRCGWIDLVALNFACMINGVTKIVMTKADVLDA
                     FKELEVCTAYMLNGKESQEVPFQMERQKPIPVYKAFTGWNTTTSAAKTYSELPDTMKT
                     YVNFINKYLGVDIHFISNGPGRDQIIQVK"
     gene            complement(57240..60254)
                     /locus_tag="PLO70_00295"
     CDS             complement(57240..60254)
                     /locus_tag="PLO70_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ72918.1"
                     /translation="MRLRLLKVFAVLGFVFLFSKEIAAQNCIPTNINGAVINRDCSQI
                     CNALTFQIPHIKSSSDYTLVTTPYAPYPYSTPTGATDIALYNDDQYSSLINLPFTFCF
                     YGQSYTSAAVGSNGIVTFDPANAACSNAWPITETIPYAGGNICTSFPAYYPRASIMGA
                     YSDLDPRTVASPSTRKIQWEVIGTAPCRKFVVSYYHIGVFGVSASSCPVPANTFQMVM
                     HESTGIIEIFFQQKSCASSTNAGRAILGIQNWNQNQAIAAPGKNNTVWNESNTGYKFI
                     PSAGTSRYVTSELLTMGGTLVATADTSTTTTGLLDLSFPNVCVNGSSTNFVVRTTYSS
                     CDGTQNQLISYDTVTVNLTQMQAGAASAATSCNGSADGTITVTPTTGTGPFTYTMDGG
                     TPVTGPAPYTFTNVPPGAHTIFVTDAFGCVTPLIPVTVVAGPPISTTASKTDALCNGS
                     ATGTITVVQPMVGAAPFEYSLDGTTWQTSNVFTGLAAGAYTVYFREGGGCQGTLPIIV
                     AEPTVLATSVSASAAICNAQSNGTILVAANGGVSPYQYSLNGTTWQGSNMFNVPAGNY
                     TITIRDANGCISTQNTTITEPAVLAANAVATNASCDGGNDGTIVVTANGGNSSYSYSI
                     DGINFQSSNQFNVAPGNYTVTVKDNLGCSTTFNTTVGLTDNLTLAQQADPVICESKSV
                     QLQLNSNATQYAWTPATGLDNASIYNPVANPTVTTQYIVTATLGRCSANDTVTVNVNA
                     APVPNAGVDGYICYGQTYQLSASGGTQYSWSPSTYLDDANSANPVSTPTKDITYTLSI
                     LSDANGCASLVTDNMRIDVTPPIKVKTYPYDTIGYPGDQFQILVVPSDSDVINYTWNP
                     TIGLSNPNSTIGTGNLNIPNPIVTIGAIGEDIQYQVITSTIAGCRGEGYVKVRVYKGP
                     DIYVPTGFSPNNDGKNDKFTPFPVGMKSYNYFRVFNRWGQMVFSTTKLNDGWDGRLGG
                     QEQPSGVYVWMIEGLTKDNRVITKKGTVMLIR"
     gene            complement(60408..62651)
                     /locus_tag="PLO70_00300"
     CDS             complement(60408..62651)
                     /locus_tag="PLO70_00300"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQZ72919.1"
                     /translation="METVAQIPKYNLNEEEEKKLILREYRSLLKVIKSKLKPGDKALV
                     RTAFEMSAEAHKTMRRKSGEPYILHPIAVAKICAEEIGLGIRSTICALLHDTVEDTDI
                     SLDDIKREFGEEITRIVDGLTKIANVIDVNASQQAENFKKILLTLTDDPRVILIKLSD
                     RLHNMRTLDSMKREKQLKISSETVYVYAPLAHRMGLYTIKTEMEDLAMKYMEPDTYKE
                     IARKLAETKKERTKYINEFIKPIRDKLEKANLTAEIHGRPKSIHSIWNKMKKKGVGFE
                     EVYDLFAIRAILDSPPEREKEDCWKVYSMITDEYNPSPERLRDWLSNPKANGYEALHT
                     TVMGPQGKWVEVQIRTKRMNEIAEKGLAAHWKYKEGATDESRFDKWFQQIREVITNQE
                     TDSIDFLQDFKTSFLAEEIYVYTPKGDVKMLPVGSTALDFAFAIHSAIGVKTIGAKVN
                     HKLVPISQKLHSGDQVEIITSNKQKASEDWLGFVVTSKAKGRIRDALKEEKRKVADEG
                     KYAVQRKLEGMGASLSQHNIDELVHWYKLGSNLDLFYQVAVKNIDLKDIKDFKIAGDR
                     IEAPRPVKLHNEKHDFLPHHVTGKKDAELVIFGESSDKIVYNLANCCKPIPGDDVFGF
                     VTTGKGLTIHRTNCPNAAKLLANYGHRVVKTKWAKNKEISFLTGVKIVGMDDVGVVNK
                     ITNLISGELKINIAALTIEANEGLFKGNIRLYVHDKEELDNLVQSLLSLSGIESVERY
                     DMEDIPS"
     gene            63189..63270
                     /locus_tag="PLO70_00305"
     tRNA            63189..63270
                     /locus_tag="PLO70_00305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63223..63225,aa:Leu,seq:tag)
     gene            63349..64725
                     /gene="tig"
                     /locus_tag="PLO70_00310"
     CDS             63349..64725
                     /gene="tig"
                     /locus_tag="PLO70_00310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQZ72920.1"
                     /translation="MATVTKENIGLLHEKLTVKLEKTDYLPSFEKALKEYSKKANIPG
                     FRKGMVPSGLIKKMYGPSLFTDEILKSVDRELIGYLQNDKLDIFAQPLPLESDFRQLD
                     VNNPADYTFHFEIGMKPEFQLPDLAKAKTTRYVVTVTDEMVDNEITRLQNRYGNMKDE
                     ETVISEENVLNVNFTEADADGNEVEGGIKKDNSILVKYFAEGFRKNWMGKKTGDTATA
                     QLRTAFDEKEREWIIGDLGLNKEDAGTADKYFKIEITKVGLMEKRELNEEFFNQLYPN
                     QEVKTEADFRNKIKEEIQAYWNGQATNQIHDQVFHQLVDHTKIEFPEGFLKKWVKTQG
                     ENPKTDEEVENEFPKFLSQLKWTLISDKIVQENAIEATPEEIRNFAKQQLFSYMGGAN
                     LSDDQPWVNDYVEKMMKDRKYVEDAYNRIQTQKIFEWAATKLNPTDKDITADEFTKMV
                     ETHQHHHH"
     gene            65055..65651
                     /locus_tag="PLO70_00315"
     CDS             65055..65651
                     /locus_tag="PLO70_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQZ72921.1"
                     /translation="MTPNIIEERSLNVAVMDVYSRLMMDRIIFLGYPINDEIANIVTA
                     QLLFLDSSDRSRDINMYINSPGGSVYSGLGVYDTMQYVSPDIATICIGVAASMACVLL
                     GAGTKGKRAALKHSRIMMHQPSGAIGGQASDIEITVNEIRKLKRELYEVVNVHTGKSI
                     EQIEKDFDRDNWMTAAEAKEYGLVDEVLLINPRKQKKD"
     gene            65690..66319
                     /locus_tag="PLO70_00320"
     CDS             65690..66319
                     /locus_tag="PLO70_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQZ72922.1"
                     /translation="MNRKQFLYADWRMDEEEEKEEQPKSDLGMFSKKLERYFFEKRAV
                     YLWGVVDDKSAKDVVSKLLLLDADKPGEEIKFYINSPGGVVTSGMVMYDTMQMIQSPV
                     STICMGLAASMGSILLSGGKKGSRYIFPHGEVMIHQPSLGGYIQGVSTDLEIQAEQTR
                     RVKEIGAGILARNCNKTVEQIMKDFDRDYWMNANQAIEYGIADKLVEKL"
     gene            66447..67691
                     /gene="clpX"
                     /locus_tag="PLO70_00325"
     CDS             66447..67691
                     /gene="clpX"
                     /locus_tag="PLO70_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQZ72923.1"
                     /translation="MAKNPLHCSFCGRSRDEVKILIAGQEGHICENCVEHAREIIDQE
                     LAVKDDKSTSSFKLTVKKPIEIKKFLDEYAIGQDEAKKVLAVAVYNHYKRLQQKTADL
                     NSGNEVEIEKSNIVMVGETGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVES
                     ILTRLLQVCNYDVSAAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGMLKLLEGT
                     DVLVPPQGGRKHPEQKLIKINTQNILFICGGAFDGIDKIIARRIQTNTIGFNVDKELQ
                     DNMKKNLLSYVNATDLKSFGLIPELLGRLPVITHLEPLDATTLRAILTEPKNALIKQY
                     TKLFELEGIQLTIEEEVLNFMVEKAMEFKLGARGLRSICESILTDAMYELPSSKETHF
                     NLDLEYAKRNFDKSKLSLLKVA"
     gene            complement(67759..70179)
                     /locus_tag="PLO70_00330"
     CDS             complement(67759..70179)
                     /locus_tag="PLO70_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72924.1"
                     /translation="MRINKKYITTLVIMLMHYTLFAQSWIPLDPDDFNQPSYFNPTTF
                     TSIAIDTAAGIPYLVYRDGGNGNKATVARYTSGSWQIVGSAGFSSTQIEYAQIAIAPD
                     GTPFVVYQTQNGGDPFRLDATVMKYSSGTWSVVGSVNAQQIAFSGFPDYYPNIAIAIA
                     PDNTPFVAYGDYTLAVAGTVKKYDGANWVTVGAAGFTGSSQAMALSLAIDQNDGTPYI
                     LFRDDQATTQYAATVMKYDGANWVLVGARGFSNNAIIKYPSIAITSDGIPYVAYQDVT
                     RSGKANVMLFNGANWVNVGAVIGFSTGAAEYVSMAIDPQDFTPYVSYKDVANGNKATV
                     KKFNGVSWVNAGTAGFSLGGVQYTTMAIANDGVPYVVYQDATNNSRATVKKLEADWTT
                     VGATGLSEGPAGYTSAVFGTDGYMYVGFKGSSNKATVKNYVSGAWNLAGNADFSSGTA
                     NFISLSFNAADGKPYVAYQDGANSGKATVKFLAPDNAWTTIGTEGFSTGSASYTSLAI
                     NTTGTPYLAYADADVSFSNRVIVKSFNGSSWVNTGAQPISVGSASFVSLALSPGGTPF
                     VAYNDADVSIGNKAIVKTYNGSSWVNLGTQPVSAGVATFIVLGIAPGSGNPYIGYADA
                     SNSNKVTVKTYDGAAWVNVGPAGGFSTGAATNLSLSFTADGTPYVAYQDAANGNRLTI
                     KRFTAGTWTTVNAEGISAGVANNISLVIGNDIPSVAYTDGYAWVKSVCSAAGINWVGS
                     FSDAWENPLNWSCGILPDANSEVIISSGSPFLPVINSNVSVKKITVNNGASLTVTTGF
                     TLTLTGTN"
     gene            70431..70577
                     /locus_tag="PLO70_00335"
     CDS             70431..70577
                     /locus_tag="PLO70_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72925.1"
                     /translation="MQELIEKLKAEAGLTDEQAKKAITTIKNFVVEKFPMLEGAVNNV
                     FGGD"
     gene            complement(70621..70746)
                     /locus_tag="PLO70_00340"
     CDS             complement(70621..70746)
                     /locus_tag="PLO70_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72926.1"
                     /translation="MRNRVLFLLLIGSIIFQSCSRAMTPYDAANHPRGKKCATIK"
     gene            complement(70986..74366)
                     /locus_tag="PLO70_00345"
     CDS             complement(70986..74366)
                     /locus_tag="PLO70_00345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQZ72927.1"
                     /translation="MANKKQASAKSGLQFSRRFTREDVNVYDMFEYDYRTSVIRNPSG
                     EVVFEMNNVEVPKQWSQIATDILAQKYFRKAGVPQPDGSLGRETSAKQVAHRMANCWR
                     VWGERYGYFGDEKDAQVFYDELVYSILNQMCVPNSPQWFNTGLYESYGITGKPQGHYY
                     VDPNDGQLKKSQNAYERPQPHACFILSVDDDLVNEGGIMDLWVREARIFKYGSGVGTN
                     YSNLRGSGEKLSGGGSSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPEIVE
                     FINWKVEEEKKVGALIAAGYPSGYEDEAYRTVSGQNSNNSIRIPNSFFEKLDANEDWE
                     LTGRMDGRVMKKIPARELWNQIAYAAWSCADPGTQYNTTINEWHTCPEGGEIRASNPC
                     SEYMFLDNTACNLASANLMKFFDAETNQFDVEGYEYNCRLWTVVLEISVLMAQFPSKE
                     VAQLSYEYRTLGLGYANLGTMLMVSGIPYDSEEARAIAGAITAIMTGTSYKTSAEMAQ
                     VLGAFPRYEENKAHMMRVMRNHRLAAYDADEYENLSLKPQGITVKYCPDYLLKAACKA
                     WDDAVELGEKYGYRNAQATVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKI
                     INQSVPAALKNLGYFEKESEAIIKYATGSASFAGAPFINHQTLSEKGFIADEIKRLDA
                     AALTAFEIGFIFNKYTLGEECLERLGFTPEQYNNFEWSLLEALGFTDEQIEAANDYVC
                     GTMMLEGAPGLKEEHLPVFDCANKCGKKGQRYIHAHGHIRMMGATQPFISGAISKTIN
                     LPHEAKVEEIADAYYLSWKLGLKANALYRDGSKLSQPLSNKSDKKKKADIVAEEEKAV
                     VIEQQVESNIVDLGKLTVEELLEEVQKRVQASPDTKLKRKLASIVERRTLPAKRRGFT
                     QKAKISGQAVFLRTGEYADGTLGEIFVDMAKEGATMRSMMNCFAISVSIGLQYGVPLE
                     EFVDKFAFTRFDPSGFVEHPNIKTTTSIVDFIFRVLGYEYLGRTDLVHVLDKPEVMNT
                     GGDDWDEVPSNPLEYVKDPELSSVRITPSSGTVAPQPVRNQRASQPVKTESSIDAMNA
                     VAKNMQSDAPACNVCGNIMMRSGACYKCLNCGNQGGCG"
     gene            74797..75525
                     /locus_tag="PLO70_00350"
     CDS             74797..75525
                     /locus_tag="PLO70_00350"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQZ72928.1"
                     /translation="MIKSILTALIIVCSPFFINLHAQAQFTGWLASFNTIKTGKNTSL
                     HTDIQFRSTNQWQQTQTILLRPGINFHLNKRIIATAGYAFINNKRIMGNVAGFAPEHR
                     IWEQLLFNHKLKTIFISHRFRIEQRFLSKVIIVNNELETNGSVYANRFRYFNRNIFPF
                     KKEKAFSTGMFAALQNEIFINIGNTANVNGEIFDQNRLYLAIGYRPHPKADIEIGYMN
                     QYVNGKGNLFTNNHILQLAGYLRL"
     gene            75596..76351
                     /locus_tag="PLO70_00355"
     CDS             75596..76351
                     /locus_tag="PLO70_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HQZ72929.1"
                     /translation="MKSTIYNTLADKKKQGKKSFAVLIDPDKVNTSVLDELIELSISA
                     KVDYFLVGGSLVISNHLDECVQHIKQNCNIPVILFPGSPSQISKYADALLYLSLISGR
                     NPELLIGQHVVSAPFVRQSGLEIMPTGYIVIDGGAPTTVSYISNAAPVPADKNEIAMC
                     TAMAGEMLGMKLIYMDAGSGAKRPITESMIKAVADHIEVPLIIGGGIIQPEKAYLNCK
                     AGADVIVVGNAIEKDASLIKEMSDAVHSVPVKA"
     gene            complement(76348..77673)
                     /locus_tag="PLO70_00360"
     CDS             complement(76348..77673)
                     /locus_tag="PLO70_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQZ72930.1"
                     /translation="MRKFILMGLLTATTSFLLIISCGTTENAASAPVPGIDKVINVKE
                     VERIEKTLASDAMRGRRAFTPDIDRAAEFIAAEFKSIGLEIWNNNNNYRQEFAMVRTK
                     NIATSGNFDGTVIDEKNIIVITSQSHLKINQSSGYEKVSIKAGTNLQIEARKMVRSNK
                     NLLVLVDKSFSNSFGNISRLKSSMFKAYTNVVFVLSDTDPARFDIESTHEITEQKLAN
                     IVAVLPGKSKKDEYVIFSGHYDHLGVGKPVNGDSIYNGANDDAAGITALIMLSNYFKQ
                     VNNNERTIVFAAFTAEEVGGFGSQYFSRQIDADKVMAMFNIEMIGTESKWGKNSAYIT
                     GYEKTNMGEILAKDLKGTAFAFYPDPYPQQQLFYRSDNATLARLGVPAHTISTSKMDV
                     EPHYHKLTDHVETLDLTNMTEVIKAITLSSKSIIAGKETPTRVKVDELR"
     gene            complement(77754..78482)
                     /locus_tag="PLO70_00365"
     CDS             complement(77754..78482)
                     /locus_tag="PLO70_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HQZ72931.1"
                     /translation="MKAIIIDDERLARLELRKLLQEFPEIEVIDEAVNADEAISKIEA
                     TQPDLIFLDIQMPGKTGFDMLAELDKSPHVIFTTAHHEFALKAFEVNALDYLMKPVEP
                     KRLADAIQKLQISENKEIRTDTEFTNNSILSENDQVFVKDGERCWFVKLSDIRLFESV
                     GNYAKVFFANNKPLILKSLNALEERLDEKIFFRANRKHIVNLRMIEKIEPYFNGGLLL
                     DMKGGEKIEVSRRQTVKFKEMMSL"
     gene            complement(78494..79543)
                     /locus_tag="PLO70_00370"
     CDS             complement(78494..79543)
                     /locus_tag="PLO70_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ72932.1"
                     /translation="MSKNKLYWILQVGGWGLVGLVMLLFAHIYDVQISTPILLKRITI
                     VFFAGIITTHALRGVIKWSGWMLLPVEKALPRLIIAVLFTSILFSVLILGSIEVFNLS
                     TESKQNISFFQKVLGSTIDNGLFIFPWLMIYYFYHYYNKSRRQQLDTLKLESLVKELE
                     LKTIKAHINPHFIFNSLNSIRALVDENPQRARKAVTELSNILRSSMQTEKMETVPLEK
                     ELHIIKDYLALENMRFEDRLKVEYDIDEDTLDQPVPPMMLQTLVENAIKHGISQQING
                     GVIKVISDFKGDYHELVVQNTGQLNGSVNTEGFGIPSTTNRLNLLYGDKASFAIKQIN
                     GSLVEAKVLIPVEIH"
     gene            79735..81669
                     /gene="dxs"
                     /locus_tag="PLO70_00375"
     CDS             79735..81669
                     /gene="dxs"
                     /locus_tag="PLO70_00375"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQZ72933.1"
                     /translation="MQITPGSLLQTIDSPADLKKLSREKLHQVCDELRQYIIDVVSVH
                     GGHFGASLGVVELTTALHYVYNTPYDQLVWDVGHQAYGHKILTGRRDNFHTNRKYKGI
                     SGFPKRSESEYDTFGVGHSSTSISAALGMAMAAKYKGEKDRKVVAVIGDGSMTAGLAF
                     EGMNHAGVADADMLIILNDNGIGIDPNVGALKEYLTDITTSPTYNKVKDDVWKLMGKL
                     PVGKNFTRAMAHKLAEGMKGMVSSSANLFEALKIRYFGPIDGHNVAKLVDTLKDLRDI
                     PGPKILHIITTKGKGYALAEKDQTKWHAPGLFDKVTGEIQKKIFDLPQPPKYQDVFGY
                     TMIELAEMNDKIFGITPAMPSGSSLKFMMEKMPDRAFDVGIAEQHAVTVSAGMATQGL
                     RVFCNIYSSFMQRAYDQVVHDVAIQKLPVVFCLDRAGLVGDDGPTHHGCYDIAYFRCI
                     PNMIISAPMNESELRSLMYTAQLESTTLPFVIRYPRGEGVMPEWKTEMKEIEIGKGRK
                     LKEGKDIAILSFGHPGNFAAAAIRDLKNDGLNPGHYDMRFAKPLDETLLHEVCRQYQK
                     LVTVEDGSVEGGFGSAVLEFMAQHGYKNDVKILGIPDNLVEHGTPKELHRECGYDTQG
                     IIETVRAMMKVKVTADQLIS"
     gene            complement(81743..82153)
                     /locus_tag="PLO70_00380"
     CDS             complement(81743..82153)
                     /locus_tag="PLO70_00380"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQZ72934.1"
                     /translation="MKSYSSANWKGSGKEGSGTLTSKSKVLENTSYDFRSRFEDGTFT
                     NPEELVAAAHAGCFSMKLSFVLGAAGFTPDNIDTKCTITLENGAITSSHLEVKASVPG
                     IDAAKFAECAAEAKANCPISKLLNTEITMEANLA"
     gene            complement(82195..82980)
                     /locus_tag="PLO70_00385"
     CDS             complement(82195..82980)
                     /locus_tag="PLO70_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQZ72935.1"
                     /translation="MQNTSYQIKLPQFEGPFDLLLFFIERDELDIYNIPITSIINDFL
                     DYIHKSEELNIELSSEFILFISTLMRIKAKMLLPRKELDAQGNEIDPRQELINKILEY
                     KRFKEASAQMAEMEALRMLMVKRGNIQKELSQIGEETGEGTEIQNITLFKLMRAFEKA
                     MQKYSDRINKPVHTVVSYNYTMEQSRDQMLNLAREEKTVSFEKIFERCENRVHAIFLF
                     LSLLELVQQKFMKIMIGEGKNNFIIEYNEPENRIAELEEQNQQ"
     gene            complement(83084..83455)
                     /gene="mscL"
                     /locus_tag="PLO70_00390"
     CDS             complement(83084..83455)
                     /gene="mscL"
                     /locus_tag="PLO70_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQZ72936.1"
                     /translation="MGMLKEFKEFAMKGNVIDLAVGVVIGAAFGAIVGAVVDNIIMPV
                     VGMITGGINFDALSVKVGNAELKYGLAISAAVKFLAIALFLFMVIKAVNASKKKEAAA
                     APPPPPEDITLLREIRDALKK"
     gene            complement(83518..84264)
                     /locus_tag="PLO70_00395"
     CDS             complement(83518..84264)
                     /locus_tag="PLO70_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HQZ72937.1"
                     /translation="MNKVELKKIDTRAPKDLDKKELKEKTEKILVELDELQNLLFAEG
                     KHSILIVIQGMDGSGKDGVIRNVLGNMNPQGVTVKSYKAPTPEELSHDFLWRIHQHAP
                     GKGMIQVFNRSHYEDILVTRVHKWCDDETARKRMEAINDFEGLLQKHNSTHILKFYLH
                     VSPEEQHERLSERMKDPAKMWKYNDKDFEEAKLWDEYMKMYEDCFNNCNTPPWTVVPS
                     DQNWYKEYIIASQLYELLKNLDMQFPGLKK"
     gene            84347..85264
                     /locus_tag="PLO70_00400"
     CDS             84347..85264
                     /locus_tag="PLO70_00400"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQZ72938.1"
                     /translation="MSNKFISWVIFVILCFIWGSSFKLMKDSAIALNASQIASLRIFA
                     AGVVFIPFAFFHFSKIPRQKMGTVILSAVFGNLIPAFLFAGAMTKIDGSLGGILNSLT
                     PICVVVIGILFFKDKIKAQKITGVIIGFIGLVLLTISPVLIGEKNISFDNMNYLFLIL
                     GATLLYGLNVNMVSHYLKGLNPIHVATVSLAFMTIPAGIILWQQDVTQLDFHKIEVRY
                     AVMASAGLGVLGSAIATALFYILVQKAGGLFASLVTYGIPFIALFWGFLDNEKISVIE
                     IVCLMIILFGVYLANRSEKKKATPADVAS"
     gene            complement(85283..85846)
                     /gene="frr"
                     /locus_tag="PLO70_00405"
     CDS             complement(85283..85846)
                     /gene="frr"
                     /locus_tag="PLO70_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQZ72939.1"
                     /translation="MSEDIASITSNAEDFMKKAIGHLEAELVKIRAGKANPQMFDGLM
                     VDYYGSPVPVNQVSNISVMDSRTLSIQPWEKNMLQPIERAIIAANMGITPQNDGVLIR
                     LFLPPLTEERRKELVKKCLGEGEHSKVAIRNIRRDAIEHIKKLQKNGLSEDAAKDAED
                     EIQQITDKFIAAVEKHLATKEKEIMSV"
     gene            complement(85994..87130)
                     /locus_tag="PLO70_00410"
     CDS             complement(85994..87130)
                     /locus_tag="PLO70_00410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQZ72940.1"
                     /translation="MLDILLTASVSFIITFLAVPVVLEVAEKKKLFDMPNERKVHTRL
                     VASLGGVGIFGGFILASLLSIQGHLNSEFQYFFAAALVIFFLGLKDDLIILSASKKFI
                     GQLIAASILIHLGGIRLDSMYGLMGFENLPEGFALALSYLTIIVVINSFNLIDGVDGL
                     AASLGILTMLVFGSYFFAIGFQAYALLAFAMAGSLGAFMIFNHHPAKIFMGDSGSLMI
                     GLINAILVIKFINVANDPLVAIPVSSAVAIGFAILIVPLLDTLRVFSIRIFNGVSPFT
                     PDRNHVHHLLLDRGLDHASVTLTCVSANIGFIMIAWFGRSLGPNYLLLIMLTLAFSGL
                     AILYYYKRPRRAILIAKRGEGVAQIKTSSTKIVTLTKEAQSAVD"
     gene            87372..88205
                     /locus_tag="PLO70_00415"
     CDS             87372..88205
                     /locus_tag="PLO70_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQZ72941.1"
                     /translation="MNELAAVASQIRRDIVRMVHGVSSGHPGGSLGCTDFLTALYFKV
                     MKHNPVFDMNATDEDVFVLSNGHISPLFYSTLARSGYFDIKELASFRKLNTRLQGHPA
                     THEHLPGVRIASGSLGQGMSVAIGAALTKKLNGKKNLVFSLHGDGELDEGQIWEAVMF
                     AAHHKVDNLISTIDWNGQQIDGPTNKVMNLGNIREKFDVFGWTTLEMNGNDMDEVVAT
                     LEKAKSLTGQGKPVAIMMHTAMGKGVDFMEGTHEWHGIAPNDEQLAKALAQLPETLGD
                     Y"
     gene            complement(88296..89519)
                     /locus_tag="PLO70_00420"
     CDS             complement(88296..89519)
                     /locus_tag="PLO70_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQZ72942.1"
                     /translation="MNFLFAWRYFKAKKSTNAINIIAWTSITAIIIGTTALILVLSVF
                     NGFEGLVKSLYSSFYTDLKVSPVSGKMITVTPEQIKQLKGVAGIRNFSMILEEKAMVQ
                     NGDNQTVIYLKGVDENYRYITGVADHLINGEYNIGNEEVPRLVLGAGVEDALGIYADR
                     NIFTLKIYLPRKSNSKQIDIMEDISNDTIQSSAAFLIQQDFDNKYGITNIDFVRKSLR
                     LTANVYSGVEIALKDPSKMKVIRKELGGIFGNGYLIQDRYEQNRSLFSIMNIERWIVY
                     GVLCLILVVAAFNMIGALTMLVLEKQKDISVLHALGGNRSFIQRIFLSEGMLLALIGG
                     GIGMLLAFLIAWLQIKFHLIPLQGGSFLIDYYPVKLRGIDFLLVGATVFIIAMVASWL
                     PSRKAANQNFSLRSE"
     gene            complement(89558..89929)
                     /locus_tag="PLO70_00425"
     CDS             complement(89558..89929)
                     /locus_tag="PLO70_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HQZ72943.1"
                     /translation="MEEGKRQKQIAGLLNEEMNFIFQRLGLNMIDGGMVSISSVKVTP
                     DLLEARFYLSFFQVKDISSALKKIEDRHHEIKKELAAKVRHQLRSIPVLKFFQDDTLD
                     HVFKMEELFKKIEEEKKGNNG"
     gene            90023..91828
                     /locus_tag="PLO70_00430"
     CDS             90023..91828
                     /locus_tag="PLO70_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ72944.1"
                     /translation="MKQYSRIFQYLGAYKGKIFLYFLSTLLSIVFSIVSIGMLMPFLQ
                     LIFTGSQTALVAESSNPVIQWVNDFLNNSVLTRGKIATLGLICLLMVSFIALKNVFLY
                     LSYYVLNPLKNRIVNQLREDLYDKVLRLPIGFFNEKRKGDLMSRMTNDVSEVESSVVG
                     TLEGWIRDPMTIIVTLTVLFLISVQLTLFILILIPVLGLVIGRVTKSLKKHSQDVANK
                     YGETLSTLDETLGGLRVIKAFNIEKVLRSKFFKSNEELLYSKNKISYRRDLASPLSEV
                     MGVVLFTAVLYYGGRLVLQNQLLEASAFLGFLGIFYNIINPAKALSTSFSNMRKGAAA
                     INRIEEVLKTPITVDDNPKGRLLPAFTDKIEFRNVSFAYDDAMILDNINLTVKKGQTI
                     ALVGSSGAGKSTLADLVPRFHDVTGGEVLIDGINIKDYSLHSVRSLMSIVTQEPILFN
                     DTIGNNIALGQQGATEEQIHEAAKIANAHEFIIKKEGGYNSNIGDRGSKLSGGERQRL
                     TIARAVLKNPPILILDEATSSLDTESERLVQDAINNMMQNRTSIVIAHRLSTIRHADE
                     IIVLQKGKIVERGNHEQLLAQNGFYKKLVDMQEVK"
     gene            complement(91898..93037)
                     /locus_tag="PLO70_00435"
     CDS             complement(91898..93037)
                     /locus_tag="PLO70_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQZ72945.1"
                     /translation="MNFQSAELTQQVAQTAKDFAQQHIKPHVMEWDESQEFPVHIFKE
                     LGKLGMMGVLVPHEYGGAGLTYFEYKTIIEEIAKVCGSIGLSVAAHNSLCTGHILSFA
                     SEEQKKKWLPKLATAEWIGAWGLTEANTGSDAGNMKCTAVKDGNDWVISGTKNWITHG
                     KSGDVAVVICRTGEPRAKNNSTTFVVEKGTAGFSAGKKENKLGMRASETAEMVFDNCR
                     IPDTNRLGEVGDGFKQAMKVLDGGRISIASLSLGIAKGAYEAALKYSQERYQFDKPIS
                     SFQGISFKLADMATEIAAAELLTLQACDLKNRHLPMTKEAAMAKYYASEVAVRVANDA
                     VQVFGGYGYTKDFPVEKHYRDAKLCTIGEGTSEIQKLVISREVLK"
     gene            complement(93212..93991)
                     /locus_tag="PLO70_00440"
     CDS             complement(93212..93991)
                     /locus_tag="PLO70_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ72946.1"
                     /translation="MICPPLKITFLGTGTSSGVPMIGCDCEVCTSSDEKDKRLRSSIL
                     VQSAKTTLVVDTGPDFRYQMLRQKVKKLDAVVFTHPHKDHLAGLDDVRAYNFFTKRHI
                     DIYADSLTEEAVRRDFYYAFTDTKYPGIPELNLNTISSEPFVVGDIPVTPVSVWHLKM
                     PVLGFRFGKFTYITDANRIDDKELERIRGSEVLVLNALRKQKHISHFTLEEAIEVVQE
                     LKIPTAYFTHMSHQIGLHAEIEAELPDNIHLAYDGLELDFK"
     gene            complement(94067..94348)
                     /locus_tag="PLO70_00445"
     CDS             complement(94067..94348)
                     /locus_tag="PLO70_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72947.1"
                     /translation="MKYTQTTLDKLEAIPEEAGYVLRYERGTFQSGYCILESKKVVVL
                     NKFLQIEGRINTLIDLIPQLEINTELLTEESKKLYTGIISKLEKEENGN"
     gene            complement(94461..96017)
                     /locus_tag="PLO70_00450"
     CDS             complement(94461..96017)
                     /locus_tag="PLO70_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HQZ72948.1"
                     /translation="MAVAKIIWVDDEIESLQSQKLFLENKGYEVQTFTNGFDAIDYVK
                     ENPVDVVLIDESMPGITGLETLAKIKEINQSLPVVLITKNETENLMDDAIGSQISDYL
                     IKPVNPNQVWLSLKKIIDNRRLVAEKTTTAYQQQFRHLFTALNSNPDYNEWMDIYKKL
                     VFWELEMEKSDSPEMQEILQNQKSEANTEFFKFVSKNYASWVSPKSTDGPVMSHTLLK
                     FKVLPHLEKGTPLFFILIDNLRFDQWKAIQPIFAESFRILEEESFYSILPTATQYCRN
                     AIFAGLMPVEIEKQFPLQWKNDEEEGGKNLHEEEFFRAQLKRLGKNDLKVSYTKVVNN
                     QAGLDLVNNIHNLLNNDINVIVYNFVDMLSHARTEMEVLKELAGDEMSYRSITASWFE
                     HSPLHQALKKVADKNIKLVLATDHGSVRVKTPFKVVGDKQTTTNLRYKHGRNLNYEPK
                     EVLAFRDPREAGLPVPTINSSFIFAKEDSFLCYPNNYNYYVNYYRNTFQHGGVSLEEM
                     IVPVIKMQSR"
     gene            96145..97359
                     /locus_tag="PLO70_00455"
     CDS             96145..97359
                     /locus_tag="PLO70_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQZ72949.1"
                     /translation="MPSVRKIINDPVYGFITIDHPLILQIISHPVYQRLRNIHQMAFA
                     HLVYPGAVHSRLHHSLGAYHLMCMALGELKSKSIEISAEEELAAKVAILLHDIGHGPF
                     SHALENELIKNVHHETISLLLMQKLNSELNGQLAIAIEIFTNKHSKKFLHQLVSGQLD
                     VDRMDYLNRDSFFTGVAEGVIGYDRIIKMLTVKDGNLVVEEKAIYSIEKFLVSRRLMY
                     WQVYLHKTVVSAEMMLVKIIRRANELIAKGEKVAAASNELDFFLQNSEPDSSIEQHLD
                     TFCRLDDHDVMATIKNWCHHQDKILATLCRYLVERKLLKVKLQAEPFDEQFVNQKKKE
                     ACIKLEISEKEVDYFVFTGEASNTTYNPGDERINILFKDGSIKDISKVDNALIQHNLS
                     GTVKKYYICYMR"
     gene            97445..98494
                     /gene="lpxD"
                     /locus_tag="PLO70_00460"
     CDS             97445..98494
                     /gene="lpxD"
                     /locus_tag="PLO70_00460"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQZ72950.1"
                     /translation="MQFPAAQIALIVKGKIEGDPNTTVNSFGKIEEAKEGQLSFLANP
                     KYEDYLYSTKASVIIVNENFEAKQPVNATLIRVPDAYTAFATLLSKYQEIKQQQLSGI
                     QQPVFIAPTATYGQNVFIGAFAYLGENVVVGNNTKIYPNVYVGDNVTLGENSILHPGV
                     KIYHDCKVGNNVIIHAGTVIGSDGFGFAPQADGSFIKVPQIGNVVIEDNVEIGSNTTI
                     DRATIGSTLIKSGAKLDNLIQIAHNVEVGNSTVIAAQAGISGSTKIGNGVMIGGQAGL
                     AGHLHVADGSKINAQAGLGKSLKTPNTAVTGSPAYEYNKAIRSQSMARNLPELEKRVK
                     ELETIIKQLLAERVV"
     gene            98556..99410
                     /locus_tag="PLO70_00465"
     CDS             98556..99410
                     /locus_tag="PLO70_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HQZ72951.1"
                     /translation="MRKISLSLSFFFLAVTILAQQKPAYILYNAKGQQITYKKMLKTL
                     QQKDIVLFGEFHNNAIAHWLQLEVTKDCDATRDLVLGAEMFEQDNQQALDLYLQGKIT
                     AKGLDSMARLWKNYPTDYAPLVNYAKENKIAFAATNIPRRYASLVSKGGFETLDTLSA
                     QAKTWIAPLPIAYDAELPGYKKIKEMMAGHGGDNMPKAQASKDATMAHFILRYYQPGK
                     LFIHYNGAFHSENYEGILWYLKKQQPELKYATITTVSQKDIHSLLAENKGKADFIICV
                     DEDMTNTY"
     gene            99423..100295
                     /locus_tag="PLO70_00470"
     CDS             99423..100295
                     /locus_tag="PLO70_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQZ72952.1"
                     /translation="MIIHGFTFVRNAVKYDYPVIESITSILPIVDEFIVSVGNSDDNT
                     LQLIESIDSPKIRIIHSVWDDSLREGGRVLAVETDKAFARISPSADWAFYLQADEVVH
                     EKYLETIKNEAEKYLHDKKVEGLLFKYLHFYGTYDYIGNSRRWYNREIRLIRNDKTIK
                     SYRDAQGFRKAGRKLNVKLIDAYINHYGWVKHPQKQQKKRDDFGLLWSNDQQAIIKEE
                     TRDMFDYMKDADVLMPFTDSHPAVMHKRINEKNWEANFNLTKTNLSVKDRLLYNIEKI
                     TGRRIFDYRNYKII"
     gene            100351..101754
                     /locus_tag="PLO70_00475"
     CDS             100351..101754
                     /locus_tag="PLO70_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HQZ72953.1"
                     /translation="MAPDFNPDKQHTLKSPVTISGTGLHTGVMADLTLNPANPGFGLQ
                     FKRIDLPNQPIIKADCDLVTDVSRGTTLEANGAKVSTIEHVLAALVGMGVDNCLLEIN
                     GPEMPIMDGSSQPFVELIEESGVLEQDAAKVWYSIDENIYHYDEEKRVEMVAMPAMEY
                     QVTTLIDFNSPVLGTQHAGLKNIRDFKEEIAPCRTFCFLHELEMLLDNNLIKGGDVNN
                     AIVIVDKRVDDKEMERLKKIFKKDKIEVKSEGYLNNLELRFPNEPARHKLLDVIGDLA
                     LIGYPIKGRIIANRPGHSSNVDFAKKIKQYIKKNKHTKGVPVYDPSQPPVYDQQFIEK
                     KLPHRFPFALVDKIIELSDTHIVGIKNVTFNEWFFQGHFPGNPVMPGVLQIEALAQCG
                     GILAINLSGEGQYDTYFLKIDNCKFKQMVRPGDTLILKMELSAPIRRGICEMKGTTYV
                     GNKVCAEADLVAQIVKR"
     gene            101961..103940
                     /gene="gyrB"
                     /locus_tag="PLO70_00480"
     CDS             101961..103940
                     /gene="gyrB"
                     /locus_tag="PLO70_00480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQZ72954.1"
                     /translation="MSPEAKEKAARISANYSADSIQVLEGLEAVRKRPAMYIGDIGVK
                     GLHHLVYEVVDNSIDEALAGYCKNITVNIHEDNSISVEDDGRGIPTAMHSKEKKSALE
                     VVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSKRLLVTVNRDGKIFEQEYKIGVP
                     QYAVRESGTSETTGTKVHFWPDDSIFITTVYNKDILESRLRELSFLNRGVRIILNDLR
                     EKEEDGSTYTKSFYSEGGIVEFVEMLDKNAGRNSLIPKIISVEGRDENTNVAVEVALS
                     YNDSYSEHIYSYVNNINTIEGGTHVSGFRRALTRVFKSYGDKNGLFEKAKVEIEGDDF
                     REGLSAIISVKVPEPQFEGQTKTKLGNNEVMGVVDSTVSKVLEAYLEENPREAKNIVS
                     KVILAAQARAAARKARELVQRKSVLTGGGLPGKLADCSDRDPERCELFLVEGDSAGGT
                     AKQGRDRSFQAILPLRGKILNVEKAMEHKIYDNEEIRNMFTALGVKMGTPEDPKALDL
                     SKLRYHKLIIMTDADVDGSHIATLILTFVYRYMTELVEQGYVYIAQPPLYLVKKGKEQ
                     AYAYNENQRKALIEKLGGGKDDSVNIQRYKGLGEMNAEQLWETTMNPGSRTLKRVTIE
                     SAAEADRIFSMLMGDEVAPRREFIESHAKYAKLDI"
     gene            104120..104323
                     /locus_tag="PLO70_00485"
     CDS             104120..104323
                     /locus_tag="PLO70_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72955.1"
                     /translation="MSDVKITAYNVKTKEKGVEMFDAVITKTAKGAYMAQGNDGKGNK
                     LTTLMGEEKAMAAIKAGVAKQGW"
     gene            complement(104376..109799)
                     /locus_tag="PLO70_00490"
     CDS             complement(104376..109799)
                     /locus_tag="PLO70_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQZ72956.1"
                     /translation="MRFYPIPMIAAMLLFTFSLAAQDDSRYNLLLKSGSFIPSKNITA
                     DKIESFNRIAARTTGKAFALVQFEKIPTEYEKQQLLHSGIELLEYIPYNAYTVTITGQ
                     LDPDLLSRMKARAIVELTAEQKMPPALAKGLFPAHAVRIAGTLDVWISFPKSFSFETV
                     SRELQIKNFDILNVDYKTYRVITLRIAAMRLNELASLPFVEYVQTVPTADQELNYNSM
                     FASRANMLKAPLAYGGKNLNGQGVVIGIGDNGDVQSHLDFTGRLINRAGEVMRAHATH
                     VAGTVAGAGIIQERYAGYAPKATIINQYFSGIFANAPAYVQDHGMVITNNSFGTVVTD
                     CFYNGLYDLSSRILDQQAFDLPELQQVFAAGNDGGLTCAPYPAGFKTVLGGYQSAKNV
                     LTVGSTDYKSDISGFSSRGPVRDGRLKPEIMSQGQLVASTWVSNLYSYNNGTSMAAPG
                     VSGGLALLIQRYRQLNAGANPKSGLMKALLCNGGDDKGNPGPDFRYGFGRMNLLRSVA
                     TIENATYFNSTVTNSSTNTHAVIVPANTAQLKVLVYWQDPPASVMASSTLVNDIDIEV
                     VTPSSGIVLPVILDTIPANVNNAATTGADHINNIEQVVIDNPAAGTYDIRVIGSAITQ
                     NPSQEYFLVYDLIPESLVLSNPVGGESLVPSVSAFDTCYIQWDSYGGTANDFTLEFSS
                     DNGTNWTTLSNTIPAASRIYNWGVPNTPTDQARIRITKNSTAFTQTSFPFTITAMSTV
                     SLDPVQCEGYIKINWTAVNGATDYEVMMLQGDEMISVATTPLLTYTFSGLSKDSLYWV
                     TVRPRINGSTGRRANAVSRQPNNGTCTGTISDNDLKIDAIVAPASSGRIFTASALSNN
                     ESITIRIKNLDDAASAGNINVSYTINGGAAVNEIITSPVADIAAGGTIDYTFITPANL
                     SGVGTYSVQVTAAKATDPVITNNSFTKMFKQLDNQPITNTELPWLDNFESATVQTVRN
                     VQMGLDGLDRYDFVNGTVYGQARTFINTGIAYSGTKALTLDQDRYVAGGNIDSLTGTF
                     NLATFNTATDDIRLDFRYKNHGQKSNAANKVWIRGSETDSWIEVYDLFANQNPADGSY
                     KLSGSIEVSDTLSAHAQTFSTSFQARWGQWGEYLAADNESAAGYTFDDVRIYKAVDDI
                     QLVSIDTPYAVSCGLDATVPVMVTVRNTSNAVINNIPVVLKVDGNIIATEAIITIPAS
                     SAIQYTFTATADLSAVGDHNIEVWVALITDNYRENDTARAVINNLPMVTTFPYLQNFE
                     LGNGSWYAGGARTSWEYGTPVSPKINRAASGSKAWKTRIAGYYNDAEYSYLYSPCFNI
                     SGMTAPTLSLSIAMDIEDCGAVLCDAAWVEYSTDGGTVWTKLGSTGQGTNWYNKDYAG
                     NQLWSQQNYTRWHVATTALPTTNNSKLRLRFVFKSDESLSKDGLAIDDIHIYDNLNGI
                     YDGVTMTSPVTQTINAGINNWVDFLEGGKLVASIHPAGQELGVTNAQAYINTGAVRID
                     SDQFYHDRNITIQPGNSFLSLTDSVLVRYYFLDSETEALINASGCSYCYKPSTAYDLG
                     VSKYSDPDNNFENGSVADNNQGIWTFINSTRATKVPFDKGYYAEFKVNNFSEFWLNNG
                     GFDNNQPLPVQLVSFTARKANVKDVLAEWVTTSEFNVNRFDLEVAKGNTEFQQDRFMK
                     IGEVRSQGNSEREQRYNFTDIENNKNGVRYYRLKIIENDGSFSYSAIRPVVFNDELKW
                     LVTPNPSSGIFNLTYQANDGEPISVKVYDINGRVVNRFDHVSNGFVQKLTINLADAKF
                     SSGLYMLELMTGGKTHSFKLIKQ"
     gene            complement(109885..113316)
                     /gene="mfd"
                     /locus_tag="PLO70_00495"
     CDS             complement(109885..113316)
                     /gene="mfd"
                     /locus_tag="PLO70_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQZ72957.1"
                     /translation="MSVMDLLEQYQNTPRLFQLADKLSFTQPQKIFLENLQGSSSQFV
                     VAAAFLHPSLSRVNHLVILNDGEEAAYFHNTLENLTGALDIFYFPSSFKNRKNYQLLN
                     SSHVMLRTEALTKVAAGGNKKVLVTYPEALFERVVLPGTLSSNIIHIKSGDTLNVELL
                     LLKLSDYGFERTDFVYEPGQFAIRGGILDIYSFGNEKPYRVELFGNEVDSIRIVDPET
                     QLSERKLLQVSIIPNVDTQFASEERISLLDFLPENTVVWLQDYDLCKERLTDCEEDML
                     AFLRLQEGLKVQGARDKVLEEQDDKLVKKNTGENDFITVKEFEEKLFQKHTVGFGHSD
                     SFPVPFSSFTIHFSTKEQPSFNRQFDLLIKDLKNWEAKKFQVYIFAENPKQLERLQSI
                     FDDLKADIVFNPVNVSIHEGFIDEDQKIVCYTDHQVFQRYHKYKVKQAYNKNKALTLR
                     TLRELQPGDFVTHIDHGVGIFSGLQKLEVNGRLQEAVRLIYKDTDILYVNINSLHKIA
                     KYTGKDGSVPKVNKLGSDVWNKLKEKTKTKVKEIAFDLIKLYAKRKAQQGFQHTPDNY
                     MQTELEASFIYEDTPDQSKASADVKKDMESPAPMDRLVCGDVGFGKTEIAIRAAFKTC
                     VDGKQAAVLVPTTILAFQHFQTFSERLKDFPVTVDYINRFKSAKEKKETLKKLEEGKI
                     DIIIGTHGLLGKEVKFKDLGLLVVDEEQKFGVGHKEKIKTLRTAVDCLTLTATPIPRT
                     LQFSLMGARDLSIINTPPPNRQPIQTEVQVYNEDFIREAIYYETERGGQVFFIYNRIA
                     GLAEMAAIIQALCPDLSIGYAHGQMEGHELEKHILDFIDRKYDVLVCTNIVESGVDIP
                     NVNTIIVNNAHQFGLSDLHQLRGRVGRSNKKAFCYLLAPPMSTLPNDSRKRLQTLEQH
                     SELGSGFQIAMRDLDIRGAGNMLGGEQSGFMAEIGFEMYQKILDEAIRELKRTEFKEL
                     FKEEIAKQDDFVQDCTIDTDLEILIPDDFVESITERLSLYQRLDNCETEDDLLVMRQE
                     LEDRFGALPQQVEDLFITVRCRKLAVDLGFEKMSLKDNTLRCFFINRPDSPYFESGTF
                     QKVIQFLQTETNKGKLKQTGKLFLLIANNMNSMEEMHRFLLSMHNYCFSTSAIPA"
     gene            113420..114139
                     /locus_tag="PLO70_00500"
     CDS             113420..114139
                     /locus_tag="PLO70_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ72958.1"
                     /translation="MALQPWRKGIVTKIDDHTHNTRRFWIQVPEMEKFDFIPGQFVTL
                     DLPIHEKPNKRWRSYSIASWPDGTNIFELLIVHAEGGLGTTYLFSEISVGSELTFRGA
                     QGVFTLPEKIEKDIFLICTGTGIAPFRSMVNYIHLHNIPFKKIHMIFGCRNKADLLYY
                     DELTELENKLPGFHYHPTLSREHWEGHNGYVHLIYQDLCRDKQDASFFLCGWKHMIDE
                     AKKIITEMGYDRKAIHQELYG"
     gene            114253..114603
                     /locus_tag="PLO70_00505"
     CDS             114253..114603
                     /locus_tag="PLO70_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQZ72959.1"
                     /translation="MGPSSLIVLIIAAIAFSGIAILIAKFVLKASTNKQKGEPYECGI
                     PTQGKTWIQLNVGYYLFALIFLIFDVELVFLYPWAVVAKSIGWVAFIEIIIFFFILFM
                     GFLYAHKKGALKWI"
     gene            114656..115150
                     /gene="nuoB"
                     /locus_tag="PLO70_00510"
     CDS             114656..115150
                     /gene="nuoB"
                     /locus_tag="PLO70_00510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HQZ72960.1"
                     /translation="MHETPGGGIVLSKLDDVINWARSNSLWPLTFATSCCGIEYMSVA
                     GAKYDFSRFGFEVARATPRQADVIIIAGTIVNKMAPVLKRLYDQMADPKYVIAMGACA
                     ISGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALMHALITLQQKIKSGLTREQIR
                     TAKD"
     gene            115153..115611
                     /locus_tag="PLO70_00515"
     CDS             115153..115611
                     /locus_tag="PLO70_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQZ72961.1"
                     /translation="MQNEELKAKITELLSAATFEEGGEWLNINVEPADWLPLAQQLRN
                     DASLYFDYLFCLTCIDWKTHLTMVYHLSSTKHRHNIVIKSKLDRNKPEIQTVSQVWKT
                     ADFHEREVYEMFGVNFLNHPDLRLLILPDGWEGKNPLRKDFEDPINMIKL"
     gene            115613..>116196
                     /locus_tag="PLO70_00520"
     CDS             115613..>116196
                     /locus_tag="PLO70_00520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQZ72962.1"
                     /translation="MLEEVGTTTEGDLVINVGPQHPATHGVLHLVITLNGETIKKVEP
                     HLGFIHRSIEKMCESLTYRQFIYVTSRMDYLSSHINNHACALCVEKGLQVEIPPRAQV
                     IRVLMDELTRIASHELWWGAMAMDLGAFTPFFHAFREREGINDIMEETCGARLTMNYM
                     VPGGVMQDLHPNFQKKVKEFLQLYKRKIHEYDELV"
BASE COUNT        35128 a        24435 c        22670 g        33863 t
ORIGIN      
        1 tcatttcacg catcaggtca tttccttcac gggttctttc ccctacaccg gcaaacacag
       61 acacccccgc atatgctttg gcgatattat tgatcaattc ctggatcaaa acggttttac
      121 ccacacctgc accaccaaac aaaccaattt ttccgccttt tgagtagggc tcaatcaggt
      181 caataacctt aataccggta aataatacct cagtggcagt acttagattt tcaaacatgg
      241 gtggcatatt atgaatcggg cggccaccta ccttgctcac tgcaggtaat ccatcaatcg
      301 ggtcaccagt cacattgaac aaacgtcctt taataccttc gccgattggc atggcaatcg
      361 caatacctgt atccacgact tcagtaccac gaacaaggcc ttctgttccg tccatagcaa
      421 tggtacgaac actgtcttca cccaggtgct gctgcacctc aagaacaagc gtatcaccat
      481 tttctctttt cagttctaaa gcattataaa tttcagggag tttgccatcg aactgtacgt
      541 cgactacggc accaattacc tgttttactt tacctacgtt tgccatataa agtgtttatt
      601 aggagggtta cttttcaggc cgcaaaggta agggggcggg gttaatccgc caatttgaaa
      661 atcaaagatt ttcggactgt ggaaaagcct gttggaaacc ggtttactac tacccggaaa
      721 tccagtttaa agaagagtac ctattgcttt ttttgaataa tgagccccag ggatgtaact
      781 acgaacctgt caatctgtaa tttaagatgt atgtcctgct gatggggaat cgtatctacc
      841 tggaaatctt caggcatccc tccaagggag taatcctgat cctcaacaaa atgtcctgat
      901 tccctgagtt cttttatcaa acgttctata tccctgtatc tgaaaatcac cgtgtcatta
      961 ttatcctggg tctcatcgtt tgatgatacg ttgaattctg ttgtatgaac tgcccagcca
     1021 ccaggcttga ttgttttcaa ttggtttttg agaaaattca gacctttttc aattgttccc
     1081 agatgttcaa aagagcagct tgaccaattg aagtcaaatt cttcaaggtc atctggaatt
     1141 tcattcatat ccactgcccg gtatttaact ttttccaaaa acacagcttc ctcacatagc
     1201 atcctggtgt ttaatgattc gagtccgaag caaagctgat tatcagtagt ccaccctttt
     1261 tcaattcctt tttgggggaa aatatcggta gccaggatct cgcaaccata gtttgcgaaa
     1321 atagaagggg caggttcggt gccaacagcg aaaaccagtc ctttttttcc gggaccaata
     1381 caaccccgct cccacaatgc ctgcatatta tatacaaatt cccattgctt gcgatggaac
     1441 cgcggttgtt cattcatttc tgcacaccac cttttatacc aacctgtatg aaaatcctgg
     1501 gctttgcaca attcagttct ccggcgtccg gcatcttgct ggagagatat gttccatttt
     1561 ctttctgaca tgatgttgta tattttaccc ggccagtgat ttatacactg caaggtattc
     1621 ctttgctttt tgttcccagt taaactcggc acttcgcttt ttaatacgtt ctgccaaacc
     1681 aatcgcatta taatccctca ttcccttttg aaatacctgc tgcatagatt ttccttcaaa
     1741 agagttaaaa taaaatgcta catccccgcc aatttctggc aaagacgtga gggaagagag
     1801 gaataaaggc tttccgaact gcatcacttc tactaccggc gcaccaaagc cttctgccaa
     1861 tgagggatgt acaaaagcaa ggcagttttg atagtaccat gctttttcac cttctgaaac
     1921 aggccccggt aagtgcaacc tttcaatgac ccccattttc tcagcctgct tttttatatc
     1981 ttctacatat tcctgctcat ccagccggcc ggcaataatc aattctattt cttcattctc
     2041 caggagtggc agtaacacat gatagttttt tttccggttg acatacccca ttccaaataa
     2101 aaaaggccgt actggatgat atgatgtact tcttaactca ggagtatgaa ctttatgagt
     2161 accattgcga atcacataaa ccggcttatt gccgacttta caatgctgca acacatcctc
     2221 cttacaaaac tgtgatatac agacaagagc atctgcttta tcaattaaag attgtgtatg
     2281 cgaaagactt tttttctgtt cctcaatcgg cttcccttca tgcagggggt taaggtcatg
     2341 aatggttaac agcactttgg taccgggaaa ctttttctta tcaggtacaa tccttcccga
     2401 ctggaatggt gcatgccata tatcgcagga aagcgaagaa ggggaaaata aattccaaaa
     2461 agttttcttt tcaggaatgc aatttgaatc aggaccaaaa gagtttatct ctgccggagg
     2521 tacataaaat gaaatagtac cggttccggc ttccttcaat tgcgacttta ccgataaacc
     2581 caaattcaga caataatgat acaacccgga attgggatgt ttcatcagtt cgcagtcgag
     2641 aattattttc atttttctat taaccatcaa ggttccaaaa ttttcaattt cgaaatgccc
     2701 gtccattggt acgccctggc taaataaatg taacctgcat tattatataa aaaaagaaat
     2761 atagttacaa tcgggtattt tatgtttacc aatcatttgg aagggttttc tgattattgc
     2821 cgcagaaccc ataaacttgc aaacaattgc aagattttta aacaaggctg gcaatttaat
     2881 tttaacctgg gcaaatgcac aagattattt ttgattgtga acgaatgaaa tacccggaca
     2941 ctgggcttta ccactattgc ctcaaccttg gaaaatacct ggagaaaaat ataaacctca
     3001 gagaagaaca actacacttt tattctcccc cggctcagca ggattggtca tttacaacca
     3061 gcaaccacct tacacaaagc ccattccata aattcaaatt acctgcttta aattcttatg
     3121 atatctggca tgccacatac caaaacactc attacatgcc catgctgaat aaaaagataa
     3181 aagttgtgct cagcattcac gacctgaatt tcatgtatga cgaaaaaaaa tcaaccgcca
     3241 aaaagagtaa atacctgcaa taccttcaac gcctgatcaa cagggccgac gtcatagtgt
     3301 gtatttcaga gtatagtaaa aaagatgtat tacagcattg tgatatcaaa agcaagccac
     3361 tcttcgtaat tcataatgga agcaacctgc tgcaaaaacc ggagttgacg ttgggatctt
     3421 acaaacccca aaaacctttt cttttttcaa ttggtgttat ccaccgtaaa aagaattttc
     3481 atgtactgct cccattactc cagcagaata caatggaact cctgatagcc ggaaaacatg
     3541 atgatgaaaa ttatctttca tttatacaaa acaagtcaaa agaattgggt gttcaggata
     3601 atgtccattt attgggaagg gtcaccgaaa atgaaaaatc atggtatttt aataattgca
     3661 gtgcgtttgc ctttccatcc atttccgaag gatttggact tcccgttgtg gaagcgatga
     3721 gttgcggcaa acctcttttt ctatctgaca gaactgcttt acctgaaatt ggcggcgatg
     3781 tttcttttta ctttcatgat ttcaatgccg agcatgtccg gcaggtattt catgacggga
     3841 tggaattgta taatgccaac gggttgcgga aaaaaataaa agcaagaagc tataacttca
     3901 actggcataa ggcggccagg gagtacctgg aagtgtaccg ttcactttat taaaatactt
     3961 ttatctgtag ctaataatga aagtttataa aaaacttctg gcttatgccc ggccttaccg
     4021 ttggtttgta ataccatttt ttgcatttac aatactggcc gtcattttca gtgtttttca
     4081 gtttgcctta attatccctt tattaaatgt cctttttgac aacaactcag gtaaagagat
     4141 tttatcaccc cctacctttt cagtgtcggc gaatttcttt aaggatattt tttattacca
     4201 ggtgtaccgg ttaaaagctg tcaatccggt ttatgccctc tacttcatca cctttgtgat
     4261 tgtatgcgca gtgctgcttg ccaacctgtt tcgatacctg gcacagcgta ccttaattaa
     4321 agccaggaca ctgctggtaa aaagaatccg ggaagcatta tttgaaaaga tcaatcacct
     4381 ccatatggga ttttttacaa aagaacacaa aggtgacctg atctccagaa tgaattcaga
     4441 tgtatttgaa attgaaggag tggcagcaaa ttctttggaa gtattgttta aagagccttc
     4501 cctgataatc ggctattttg ttgcattatt tgctatttcc acacagctca cattgttcac
     4561 tcttatcatc attccaatat cagcattagg cattgcgacg gtacaaaaaa gattgagaag
     4621 agatgcaaag gatgcccagg cttccattgg ccgtttgctt actattatgg atgaaacact
     4681 taccgggatg cgcatcatca gggcctttaa tgccacagga tttacattga aaaaattcag
     4741 caaggaaaat gatttttacc gcaatgccag cctgcagggc ttcaagagga gagaaatggc
     4801 ccctgcattt tctgaagcag ccggtgttat tgtggttgca ggtatattgt tatatggtgg
     4861 aagtctgatc ttacaaaatg ggcaaactgc tgccggcatt caggccagtg aatttattgc
     4921 tttcatcgct attttttcac aggttttacg tcctgccaaa gcgatggtag ttgctggcgc
     4981 caatattcaa agaggccggg cagccggtga aagaattttg gaaatcattg ataagccaat
     5041 tgaagtaaaa gataaggatg atgctgcgtt attgaatgaa ttcaattatg caattgaatt
     5101 caaaaatgta gatttcagtt ataatgacat gcctgtactg aaaaatgtaa gcttcaccat
     5161 ctctaaaggt aaaactgttg cactggtagg agtttcaggt gttggcaaat caaccattgc
     5221 cgacttaatt ccccgtttct acgatgtaaa aaatggtgca gtgctggtgg atggcattga
     5281 tgtacgggat tataaaatgg aatcactccg aagtcatatg agttttgtaa cccaggagac
     5341 attccttttc aacgatacta tttttaataa tatcgccatt ggcaaaccgg atgccaccga
     5401 agaagaagta atggaagccg ccaggatcgc caatgctcat gattttatca ggcaaactga
     5461 aaatggttat caaacttcaa ttggcgaccg tggcatccgc ttatccggtg gacagcggca
     5521 aaggctttgc attgcccgtg ctgttttcaa aaacccttcc atactgatat tggatgaagc
     5581 cacttcagca cttgacaccg aatctgaacg aattgttcag gatgccctgg gtaaactgat
     5641 gaaaggaaga actactctcg ttattgctca ccgtctcagt actatcaaag aagccgatga
     5701 gatcatcgta ctgcatgaag gccagatcat tgaaagaggc catcataatg aactggttga
     5761 aattgaagaa ggagtttata aaaaactgac aaggatgcag cagatagcct gaaccgggaa
     5821 taaatatttt ctctttccta catttgcatc atgcaattga cagaagtcac aacgccacaa
     5881 ctatccaaag aatttatccg ggttaatgtt gaattgtaca aaaacgatcc caattatata
     5941 agaccattgg ataaagacat caatgatgtc tttgatcctg aaaaaaacaa agcttttcga
     6001 ttcggagagg cgacccgttg gatattaaag gataatactg gaaaactggc agggcgtatc
     6061 gccgctttta caaataaaaa atataagaat aaaggtgatg atgtacctgt aggcggtatt
     6121 ggcttttttg actgtatcaa tgatcaattg gctgcagata tgctatttga tgtagccaaa
     6181 cactggctga tacaaaaagg gatgcaggct atggatggcc ctatcaattt tggtgaacgg
     6241 gaccgctggt ggggtttgct ggtagaaggc tttcaaccac cgccatactg catgaattat
     6301 aataaatctt attaccgtga acttttcgag acctatggtt ttaaatgctt ttttaaccag
     6361 atttgttttg gactggaccc cagaaaagaa gtaagtcaaa aaatccttga caggcatgcc
     6421 cagttagcca aagaccctgc attttcatcc cgttatattg ataagaacca actggaaaaa
     6481 tttgcaactg attttgcaat tgtatataac aaggcatggg cgggtcacgg tgggttgaaa
     6541 gaattgaaaa aagaacaggt gtactcgatg tttaagaaaa tgaaaccggt catggatgag
     6601 aagattgttt ggtttgttta tcacaatgat aaccccattg ccatttttgt caacctgccc
     6661 gacctgaacc aatggttcaa atacctcaat ggcaaatttg acctctggca taaactaaaa
     6721 tttctctgga ttaagaaaac cagaaaaaac ataagattta ccgggttggt ttttggggtg
     6781 attcctgaat tccatggcac tggtgttgat gcctatatga tcaatgaggc aaaactcgtg
     6841 gttcatccga tgaacaaata tgaagaatac gaaatgcaat ggattggtga tttcaaccca
     6901 aaaatgatta atgtggcaga aaattttggc gacaccttcc gcacccggaa actgacaacc
     6961 tatcgctatt tatttgacag gacgaaagaa tttaaaccgc atccgatctt atcctaaaac
     7021 ttattcacaa agaatatcct ttttcgggca gctaggccgg gtttcttaaa ttgcaggcgg
     7081 tatggataaa ttcatcgttt cggcacggaa atacaggccg cagacttttg atacagtggt
     7141 gggacaatcg catataacca ctacattaaa gaatgcgatc aaacatcagc aactggcaca
     7201 tgcatttctt ttttgcggac caagaggggt aggcaagaca acctgtgcaa ggattttagc
     7261 caagactatt aattgcgaaa aacctactaa agagggagaa gcctgcgata aatgccagag
     7321 ttgcacctca ttcaatgatg gtgcttcgat gaattatttt gaattggatg ctgcttctaa
     7381 taactcagta gataatatcc gtgatttagt ggaccaggtt cgctttgttc ctcaaacagg
     7441 taaatacaaa gtgtatgtga ttgatgaggt tcatatgctg agttcatccg cttttaatgc
     7501 ttttttgaaa acactggaag aaccaccacc ctatgccatt tttattttag caactacgga
     7561 aaaacataaa attctcccca caatactcag ccgttgccag atcttcgatt tcaaacggat
     7621 caccaccaac gataccgttc agcacctgga agaaatatgc aaaaaggaag agataagtgc
     7681 cgaaaaggct gccctgcaga tcatcgccca aaaaagtgag ggctgcatgc gggatgcgtt
     7741 aagcatattg gataaaattg taagttttac gaatggcgaa cttaattatg ccaacacatt
     7801 agaacacctt aacatcctgg atgctgactg ctatttcaag ctattggatt gcatgcagca
     7861 gcaggatcta tccggtgcca tgttgctgta tgatgatatc aaccgcaaag gctttgaagg
     7921 agacctggtg ctcaatgatt ttgcagaatt tattcgaaat ctgctggtct gtaaagatga
     7981 aaaagcagcc gtactgctgg aagtggtaga aagttttaaa gacaagtata ttgccacagc
     8041 taaaaaagta tcgcttcccc tgctgataag tgcattaaat atattgagcg aagcggagat
     8101 taattacaaa gctgcaagga ataaacgact acatgttgaa ttaacattga tcaaactttg
     8161 ttacctgcaa caggctatcg aggtaaccgc tgatgcaaca ggtattagta aaaaaaaaat
     8221 agttgaagca cccctggctt tccgttctgt tgccatcaaa gcatttacag gttctacaca
     8281 agtaacggaa aaggcaatta cgagaccgaa taaagaaagt gaggcgaggc tgatcattca
     8341 aacccctgcc gttagtgaac ccctgcccga ttatcaacca gcctccagtc aaaaccctgc
     8401 agcaggtaat caacaaacaa aaacaggaac aaaaatttca gctcttgata agatcaggag
     8461 gcagtaccag ggaaatggca gcaacggcaa taataatatt aatcacaccc tgcaggaaga
     8521 ttcgttaaaa aaagcgtgga gtgactacat tgccaggtta aaagaagcca gaaaccctgc
     8581 ggctcagccc tttgaattag ctgtacttcg tataaaggat gaaaacacat ttgaagccat
     8641 cacagccaac aatattgagc agaaattcat cgaacaggaa agaaacacat tgtttcactt
     8701 tttacaacag cagttgcaga acaggttact gcagtttagt gtaatagtag aagaaaatgc
     8761 cgatgacagg cctaaaattg aattaacact tacggctaaa gaacagtttc aaaaaatggc
     8821 ggatcaatat ccactggtca aagaattgaa agacaggatc aggctcgagc tggactactg
     8881 accattcaca acccaattgc caacagttta ctattgctct ttttacctta atttcgacca
     8941 aaatttaaag gctaatggct gaagtgatat taatgccccg cctgagcgat accatgactg
     9001 agggagtcat cgctgcctgg cataaaaaag tgggtgatgc agtaaaaaaa ggggacctgc
     9061 tggctgaagt ggaaactgat aaagccacca tggaactgga gagctacaaa gacggtgttt
     9121 tattacatgt tggaagtgat aaaggtggta aacttcaggt gaatgatctc cttgcaatta
     9181 tcggcactcc cggggaagac atttcctcgc ttataaaaca aaactcaggg aatacagtaa
     9241 ccggaccaga aaaaaaagaa actacccctg taccggcagc tgtaccggca atagtttcac
     9301 cagccacaat cgatattgct aaaatggatg aagtggtcct gatgccaaga ctgagtgata
     9361 cgatggaaga gggagtgatt gctgcctggc acaaaaatgt aggtgacgca gtaaaaaaag
     9421 gtgaagtgct ggctgagatt gaaactgata aagccaccat ggagctggag agctataaaa
     9481 acggaaccct gttacacatt ggcgctcaaa aaggcgaaaa aattccggtg aatggcctgc
     9541 tctgtattat tggtgaaaag gggaaagtag atattgacgc tatcattgcc gccacgaaaa
     9601 gcggagttag cccacagccg gttgctcaaa gttcaaaagc ttcttccggg cctgccactg
     9661 catccactac tcaaattgtc acatcagcat caaccgaaaa tggaagagta aaagcatctc
     9721 cgcttgcaaa gaaattagca gcagaaaaag gaatagatat caaagctatc agcggttcag
     9781 gtgatggcgg cagaataatt aaagcagata ttgacaatta taaaacacca gctacagaaa
     9841 atgtgcaacc agtaacaaaa gtaactgcag cagcctcaat accgcttgct gcatttacag
     9901 gaagcacaga agagggttat acagatatcc ccaattccca gatgcgcaat gtgattgcaa
     9961 aacgtttggg agaaagtaaa ttcagcgccc cgcatttcta tcttacgatg gagatcaata
    10021 tggataatgc aatggctgcc agaacacaac tgaatgaaat aagccctgcc aagatcagct
    10081 ttaatgatat ggtagttaag gctgttgcac tggcgcttcg tcagcatcct gccattaatg
    10141 catcatggat gggggacaag atccgtcgca atcatcacat ccatattggt attgcagtgg
    10201 ccattgaaga tggattgata gtgccagtta ttcgttttgc tgaccagaaa acacttccgc
    10261 agatagcttc agaaagtaaa gagctggcag gaaaagcaaa aaataagaaa ttacaaccaa
    10321 atgaattcag tggtaataca tttaccatat ccaaccttgg catgatggat atagaagaat
    10381 ttactgctat tatcaatcca cccgacagtt gcataatggc agtaggcaga attaaagaaa
    10441 tagtagttag aaaaggagaa ggattcggtg tcagtaatgt aatgaaagta acccttagct
    10501 gtgatcaccg tagtgtggat ggggcaagtg gtgctgcatt tctgcaaact ttcaaaaaat
    10561 acctggagaa ccctattacg atgctgcttt aaagtaataa tactttattc cacaagtaaa
    10621 gctaccttat gggtagcttt tttattacaa acacttgaat gaccggattt ttattattag
    10681 ttttttatcc taaaggaatt gatagtgtat caccataaaa gaaaatgaag atatgttcgt
    10741 taagctgata agtatatttt gacattacca tacagtataa attcaatgct atggaagaaa
    10801 aagaaatgaa agatttttca tggttaacat tattctttgt taccagtatc tcttctttgc
    10861 tattcagttt cgtattaaaa ttccttgatg gaaatcattc aacatttctt ctgtggattg
    10921 gcgctgtcac tttggccatt ggattgattg ggtggctggg tacagcatta acaaagccac
    10981 ccatcaggaa aaaaagaaac ctggcatcct gatttctgaa tactttcagt ttgaaataca
    11041 cttaagtaaa acaagtttac catccattgt tgaaacaacg agctggtttt tatccagtac
    11101 ccgtacggta ttcaccatgg aattatcaat tttatgcatc caaataattt tcttctcctt
    11161 tggatctata caataaacaa caccactctt tgtaccaaag aaaacgaatc catctttttc
    11221 tatcagcatc gaaggcacat gctcatagcc aaaacctgca tgcaatttcc aggcagccga
    11281 ttgcttctta cgattggcgg catatgctac aatggtgtcc tgcatagttt tcccgtacac
    11341 ccagttacca tcttcagaaa taccaataga ttcacggact ccgccatctt tatttctcca
    11401 taatgtttcc ccagtggcca catcaatagc tgttacaaaa cgatctggtg ccataatata
    11461 aacaactcca tcagcagcca ccggtataca ggatgccgga gaataattgc gaatagctga
    11521 accattgttc catttccatt gtaaaagtcc ggtagcactg ttcaacgcat aaagatgcct
    11581 gtcccaggca ccaaagatca ctttgccatc atataataag ggagtactga caaccgggcc
    11641 atccaaccca gagaatttcc agataaactt tccattccga ctattcaaag caacaaatgc
    11701 atgatcgctg ccgccaataa atatcgtatc cccatttata actggcgaac caagaacgga
    11761 ggcaccggcc ttatatttcc attcaagttt cccctttttc aagtccagac aatacacatt
    11821 gccatcacct gaaccaaaca ctatttttcc gtttgcgact gcaggtgatg aaaaaacagc
    11881 acctgctgtt ttaaatttcc acaggggctt accatcttta attgaaaaac atgtcatcaa
    11941 tccattttgg ttaccaacga atactttctc atctgccact gcaggtgtag aaatgatatt
    12001 agcatcggat gagttttccc atattctttt tacctggtta tatttttcat taattgagta
    12061 atcaggtctt ggaaattttt tacctgtact ataattcttc aactctgctt tgacaccggt
    12121 ccatgttttc tgaaccgctg agacgggttt ccgttcagta aaaagaattg aatcatttct
    12181 tacatccacc aggttatagc ctccctcgtt tgcttttgcc cgtaagtttg accttcccat
    12241 tacacccgga atgtcctcaa aattcaatgc cctgttagaa tggccatgcc cacaaaggat
    12301 tgcccaggta ttataattac ggaggcgatc agtgacttca taccagttat ccaatccatt
    12361 atcaatcggg taatgattaa gaaatatcac cggttgttgg ggttttattt tctttagttc
    12421 ttcatcaagc cagttcaccg catcccttgg cacatgcccg tcactcatcc gcacataagg
    12481 accggatgca caacccaaaa acctcattcc attgtattca aaactgaact tatcataacc
    12541 aaaaacagtc gtgaacatga caccgccact ttcactccac ccggtatcat gattgcccgg
    12601 tataatatac cagggtatat tcaaactgtc gagtatcttt ttggcaagtt ttaattcttc
    12661 attagtcccc agttcggtga tatctcccgt cagtactaca aaaactacat ccttcatcgc
    12721 attgatatcc cgtatagttc tccgcagatc ctcttctgct gaaccattgg gagaaccaat
    12781 atgtgtatca gagataaaag caaagcggaa tggcttttgc tgtgcttctg ctaacagaaa
    12841 ccctaatagc aacagaagaa ataaaacaat ccttttcatg attccaaatt actgaattgc
    12901 aggcataccc tatgaagcat tttcaaaata tattttcaat aaattttgaa aattcggaaa
    12961 cagcactgta gatttgtccc atgaaactta cagaagcaaa acaacaattt atcagtagtt
    13021 ggggagcctt cggtacacat tggggaatca accggactat ggcgcagatt catgccttat
    13081 tattagtaag tcctgatccg cttacgcaag acgatataat ggaggagctc aacatcagca
    13141 ggggaaatac aaatatgaat atcagggaac ttattaattg gggcctggtg gaaagagtat
    13201 tgctgacagg tgaacgtaaa gaatatttta cggcagaaaa agatatctgg aaagtggtaa
    13261 aacagattgt aaaggaaaga aagaaaagag aactggaacc tatgctgcag ttactggata
    13321 aactggaaga agtggaagga gataaaagag acaaacatgt aaaaacattt gtggacaccg
    13381 tcagcagtat taaaaaactg ggtaaacaag ctgacaaaac actggatacg atgatcaaag
    13441 ccgaagaaaa ctggtttgta gggaacctgg tcaaactgtt taaataattc tccggtttat
    13501 acccactaaa gggaacaccc ttcttctatc ataattttag tatcgttatc atgcgaaaaa
    13561 aaattaaaat tgcggacctg ggaattcagg tattactctt tttggcgggt ctggttgttt
    13621 tcctttttac aaatgaagag caactttatt tttacttcta ctatttattg ggatcctggc
    13681 aaatactgag tcttataatc cactatatag ataatagagg gcttcagttc tttcaaaaaa
    13741 gacgcctatt tggccagaca atcgtatggg tcatcattac aggagcagtt agcctgttgt
    13801 tactctattt caagacgcct gtgattatct tttatctctt aggcctgctt cttttctcgc
    13861 catttcttgc tatatggtat atgagcattt gttaccgtga acttctgcaa ctaagaaaaa
    13921 gggaacttat acacttaaag taaattagat ttagttttac accatggtaa aaaagaaaac
    13981 ccttgtgctc ggtgcctctg ataatccttc gagatatagt taccttgcca ttcagcgatt
    14041 aagaaatcat ggacatccgg taacagcact tggccggaaa catacaatgg ttgaagatgt
    14101 taccatagaa acggggaaaa agcaatttga caatatcgat actgtaacac tctatctgaa
    14161 cccgcaacat caacaggaat attatgagta tatactttca ctgaaaccca aacgcatcat
    14221 ttttaatccg ggtactgaaa atgatgaact tgttaatttg gctaagaaaa aaaacataca
    14281 ggttgttgaa ggttgtacgt tagtaatgtt aagtacaaac cagtattaat tcgtagataa
    14341 acaatccagc cttcgaagtt ttacgaatca caccgggtac tactcctcaa tttcaggcaa
    14401 ttatcattac tttatcttgg aagtgtttaa aactttcaca aatcttgtat taccgtcccc
    14461 tctgaaaact gcagtagaaa atcctgactg ctgaacaata tcctattgac ttttaaaaaa
    14521 agtttaaatg aaaaacattc gtaccaccgt cctatgcgtt tgtattacgc tttacaacct
    14581 gtcatccttt gctcaggaac aaaagatccc gatcaatgaa cctgattata acaagccaaa
    14641 gttgttctcc catttaccgg ataagatccc tgtaagcatt gaaaaaatca atgaaattat
    14701 tacttcccca gtcggacgca ctactggttt ccgcctgtcg gaaacttcgg actttcagtt
    14761 tgatggtgag gttgtatcaa ctgccagtaa atatggcaat agcatccaga gtgtggttat
    14821 tcgttctgga acttatgaag gtgcaagact gaccgtctcg aaaattacca atgcagacgg
    14881 cagtatatca tatactggtc gtatcatcag ttttaaacat ggtgacctgt atgaattaca
    14941 aaaccaggac ggccaattgt tactggttaa aagaaatttt tatgacctgg taaacgaata
    15001 aatgatccgt gaaaattaat tatagctgac ccgtttttat atccgtatcc cttagtaccc
    15061 atttgatttt taaatccgcc ttgttatgaa aaacttttta caatccgtac ttacaacgct
    15121 gctgttaatc agcgttggga atgccagggc ccaggtaccg gttctgagca gtcacccatc
    15181 tgcttcagca gtattatttc tcgactttga cgggcacacc gtaagcggca ccagctggaa
    15241 ttatgcaggc ccgattgttt gtgctccttc aggactggac aataccaaaa taacaaccat
    15301 ttttaaccgc atcgcagaag actaccgacc ctttaatatt aatgtaacaa cagattctac
    15361 caaatatctg gcggcaccag ccaataagag aatgagagtg atccttactg ttacgcatga
    15421 atggtatggt tctgccgggg gcgttgcttt tgtaggctcg tattcatggg gagatgacac
    15481 accctgcttt gttttttcag gcttacttgg ttacaacgta aaaaatatct ctgaagccac
    15541 tgcacatgaa gctggccata cattgggcct ttatcaccag gcttcgtatg acgcaaattg
    15601 tgtaaaaacg tctgactacc attacggaca aggtgccgga gagatcggct gggctccaat
    15661 tatgggtgtt ggttactatc agaattttac tttatggaat aatggtccca actcatacgg
    15721 ttgcaccaac taccagaatg acctttctgt tattaccggg agtaacggtt tctcataccg
    15781 taccgatgat catactgcca atttcgctgg tgcaaccaat gctgtatttg caagcgacca
    15841 gtttaccgtt aatggtgtaa tagaacaaaa tactgatcag gatctgatta agttcatcca
    15901 accttcattc ggcaggtttc aattaaatgc tatcccttat aatgtaggaa caggaaacgc
    15961 cggttcgaac cttgacctgc aggtaacgtt atttaatggt gcacaaaccc aattgaatat
    16021 ttataaccca ggaaccttac tcagctccgt gattgatact aacctgaacc cagggactta
    16081 ttacctgaga atagaaggaa aaggcaatat gtatgcacca aactacgcaa gtcttggttc
    16141 ttacgccctg cagggaaact ttacagcagg caatacttta cctctcaggc aattgttact
    16201 acaagggcaa ttaaatggcg acaggcacca actgagctgg ataattgatg ctgatgaagc
    16261 cgtcacagaa caagtattag aagtatcaac tgacggcaga aatttcagcc cggtaacaca
    16321 atcaactaca gatgcccgtg catttactta cagaccgttt gccgctggca gcctgcaata
    16381 caggctgagt gtaactttcg ataatggtcg tcagtattat tcaaacattg taacgctgcg
    16441 caataccggt gctaatccaa aaccactgct gcttagtaat cttatcggtt caaataccat
    16501 aactgtcaca agccccggca catttgattt tgctgtctat gactttaatg gcaaaacaat
    16561 tgcgaaaggc cagctgacta acgggataaa taatattaat accaatgcga tgactggcgg
    16621 catgtatttg atccgttttg caaaagacgg tgagcaatgg actgataaaa taattcgcca
    16681 ataaagagtt ttttgttctg gtaacagaac gataatccgt gccccttttg taattcagag
    16741 gttttcctgt aacttaggaa aacctctttt tatatgcgct ggagaaaatt taatggcgac
    16801 ccgattgaac tacccatttt agatgctgtt gaacatgcca tcaaaaggga aacggagaaa
    16861 ggccaccggt tgaaagtttg tatcggcaca gactcacaag taaaaggtaa agaaacagag
    16921 tatgccacag ttattgtttt tctgcgggaa ggacatggtg gttttatgtt catccacaat
    16981 gaaaagacaa aacaacagtt caccatcaaa gaacggatgc tgacggaggt agcaaaaagc
    17041 attgagattg catatgagct gtgcaacctg tttaccattt atgatgtgga tatggaagtg
    17101 catgccgaca ttaacaccaa tccacagttc aaaagcaatg atgcactgaa agaagcaatg
    17161 ggctacatcc ttggaatggg ctttgccttc aaggccaaac cagaagcttt tgccagtagc
    17221 agctgtgcga acaagattgt aaactgagtt cctaaaactc aactggctaa tgcggggact
    17281 tcccccgcaa atgacattgt ctcccctatt ttttccttct gcgtaaacca attatcaata
    17341 aagcaagcat agcaaggccg atgctaattc gaataatagc ccctcttttc ttttcctcat
    17401 tttgttcctg ctgtaatact ctgctgtagt ctgccgtttt atctgtctgc acctgcactt
    17461 tatttatcct ggcatttacc tgtatttttt caatactgtc tttaaaatct tttagcttat
    17521 acatcaccga atcacttagc aatactctct ctttaattcc tgctttattg gaaggctttt
    17581 tcccgggtgt ctgagaaaat acaggaatgg caactataag taatatggca aatactattt
    17641 tccgcattct tttagaaatt ctgaggtaga agacatatga gaaattcaag agagggtgaa
    17701 ttattcagca gaaggagcta gttcagctct ttttaaagtg gtatgttcag ccaccagttt
    17761 ggcacttacc tggttaccgg caccagcata catactttcc atttttttgc tgtcttcctg
    17821 ggccaatgaa aaaatgataa gaaccattac aaataaaacc acaactatgt ttctttcgga
    17881 taagatcggc ttcataatta ctatgataag atggtgcaaa ttaatgcata aatgttaaac
    17941 tatctaaacc cctgcattac agcttcttaa aactgctgtt caagccgcca atataactaa
    18001 gcatttcttt tcgccctgtt tttttggttg aagaggtaac aaaataaggc ggagcttctt
    18061 cccagttttc tgacagagac tttataaagg cctccacatt tcgcacagtg gctcctggtt
    18121 tattcttatc tgtttttgta aaaactacca cgaaaggtac ctgccactct cccaactggt
    18181 taataaattc caggtcaata gcctgtgggg tatgacggct gtcaattaat acgaacaaac
    18241 acacaaggtt ttcccttttt cggatatagg actcgatcat atttccccag ttgtttcttg
    18301 ctttttgcga aactgtggca gcatatccat accctggcaa gtcagcaata ttccattttt
    18361 ttttatccga tgatataact tcaaaatgat tgatcagttt ggtctttccg ggtgtggcag
    18421 aagttttagc cagtttggtt tggccggtaa tcatattgat caacgatgat tttccaacat
    18481 tgcttctgcc gataaaagca tactccggct tgtctgcagg agggcattgt ttatgcgtag
    18541 cactgcttat tacatattta gctgatatga tttccataat gtaaaggtac aaggcaaagg
    18601 gcacaaggca gaaagtataa aaccacttct aattgaattc agtactccaa cgtcaacctt
    18661 tatatcattt aacttgttcc tgaatgttat cctttatcat tcttaacttt gcgctcccat
    18721 gtcaaatatt ttgccgcacg ataatattat tcctttcaaa gactccgaaa agagtaagaa
    18781 acagcaggta gccgatatgt ttgataaaat cgccagccgg tatgatttga tgaacaggtt
    18841 tctttctgca aggacggata ttggctggag aaaaaaagcc attcaaaagc ttaaaaaaga
    18901 caatccccag catattttgg atgtggccac cggtactgcc gatatggccc tgatggcata
    18961 taaaatgctg aaaccagcct acattaccgg cattgatatc tcggaaggca tgctggaact
    19021 gggccgaaaa aagattgaaa aagagcaact tggggataaa atccagttac atgtaggaga
    19081 ttcagaaaca ataaaatctg ctgaaaatac gtttgatgcc gtgatggtgg catttggtgt
    19141 gcggaacttt gaaaacctgg agaaaggcct cacagagatg ttgcgggtac taaaacccgg
    19201 aggtcagttg attgtactgg agttttcaaa accacgcagc aaagcagtaa aaaaatttta
    19261 caacctgtat atgggcattg tggcaccgca ggtagcccgc tggttcaaac aaaacaaaga
    19321 agcttacgaa tatttgaatg aatctgccaa agcttttccg gaccgccatc tatttactga
    19381 cattttaaaa aaagtgggct actccaacac agagtttcag ccactatcat ttggaatatg
    19441 ttgcatttac tccggaagaa aacctttata aaactcatta cgatatcacc cctggttggc
    19501 ggtcttttgc tcttttcatt tggggcatca gcacaaatgg gaaaagtgga actgaacatg
    19561 tatgaccatg atagtaagcc ctattatttt ggcattactg ttggcgtgaa cctggcccga
    19621 tttcaaacag atctgcatcc ccggttttta gactacgata gtgtgtttgt ggcagaaccg
    19681 gttaactcag gcggacttac attaggttta tctgctacgg cccgcctatc cgaccgtttt
    19741 cagctgcgtt tcaacccgca actgatgttt atcgagcgga acatttatta taaactgaaa
    19801 caccccgatc tggatggtct aactgatgtg accaagaaag ttgagtcggt aatcatgtca
    19861 ttcccttttc aagtaaaatt ccagagtgac cgaatcggca attttcgtgt gtatacgctg
    19921 gcaggattta aagccgatat agacatggcc agtaatgcaa gagctaagcg ggccgaagaa
    19981 ctcgtaaaaa ttcaaaaata tgatttcggt ccagagtttg gtattggttt caacttcttt
    20041 ttcccaagtt ttattttttc tcccgagata aaaatcagta acggtatccg taatatacat
    20101 gccagagatg agaacctccg tttttccaat gtatttgata aaatacaatc gaggatgatt
    20161 gtgttcagta ttcatttgga aggataagta tcttaaaaaa taagatctgg taaacttacc
    20221 agctgtgcat tagctgatta taaagttttt ataacgccat ttcgctttgt acacatgtta
    20281 aatatccgat gatacttttc ccggcattca ttttgtcata catccgactt tcatcattgg
    20341 ttcttagctt taactaaata cttacatata acttacacta aggacgggat atttttaaat
    20401 gccttgacga aagaaaagtc gtgtacataa actccgggta ttgataacct tttgaaataa
    20461 atttgggtaa tctgcccaac ctataacatg tacagtattg aaatttttaa tcacacctgt
    20521 taaggataat ttctgtaatt tttcattcta atacaagtta accatgccaa agtatgtaat
    20581 tgaaagagag attcccaatg caggaaaatt aaatgcggag caattaaaaa gtatttcaca
    20641 aacatcttgt ggagttctta gcaagatggg ttctgccatt caatgggttc aaagttatgt
    20701 tacaggtgac aagatatatt gtatttatat agcaccgaac aaagaaatgg ttcttgaaca
    20761 tgcaaagcag ggagggtttc ctgccaatga agtgaatgaa gtaatgacaa tcattgatcc
    20821 cacaacagca gattaactta tcaatgacaa cacaggattt gctgatgttc attaaatatt
    20881 cctgctttca ataaaatact cccccggtaa cgaaggagta tttactaatt cttaattcaa
    20941 ttattgctta gtctccccat atattatcct tgccatccag ccattgtttc actaagtctg
    21001 tagtaacaga tttcggcctt tccagcggaa aacctaaagc cctgtcccag caaagactgg
    21061 ccaatacacc caatgcacgg cttacagcaa acaatacagt gtagaattca tactccacca
    21121 tgccatagtg taccaacaaa gcaccactat gtgcatctac atttggccag gggtttttga
    21181 ttttcccgat ggtttgcaaa atcggaggta ccgtttcata aacattccat acggtctgaa
    21241 ccagtttatc ttccggcatg tgttttttac caaattccat ttgcgcagtg aaccttgggt
    21301 cagttttacg aagcaccgca tggccatatc ccggcaccac cttgccttca ctcaatgttt
    21361 tcttcacata gtcagcaatt tgctctttag aaggcaggtc tgttttcagc tcttcctgca
    21421 tctcaaatat ccacttgatc acttcctggt tggccaggcc atgcaatgga ccggcgaggc
    21481 cattcatacc tgctgcataa cttaaatacg ggtcacttaa tgcagaccct acaaggtgtg
    21541 tggtatgtgc agatacattc cctccttcat ggtcggcatg tattgtcatg tataaccgca
    21601 tcagctcttt gaaactctcg tcttcaaacc ccagcatgtg tgcaaagttt cccgcccagt
    21661 ctaataaacc attgggctga atatgttcgc tgtttttata tttacggcga tagatatagg
    21721 cagcgattct tggcaaacgt gacaataaaa ccaggctatc atcaaatacc ggcaaccagt
    21781 aatctttttt acttaatcct tcagcatatt ttttctgaaa ataactttca gtctgcaagg
    21841 ccatcactgc taccacaaac atcgtcatgg gatgagcact caccggcaaa gcgtcaattg
    21901 cttcaaatac atgggtaggc acatggcttc tgcgctgcag aatattagta acatgattag
    21961 cttcttcctc ggttggtaat tctccgatca tcatcagata aaacaaacct tcaggtaatg
    22021 gctcactacc cccgggagct ttgggaagtt tttgttgtaa ttccggaatg gaataacccc
    22081 tgaaacggat accttcctgc gcatcgagca atgatgtttc tgttaccagg ccagtaatac
    22141 ccctcattcc ctggtatatc tgtgacaatt gtacttcacc aataacctta tttccatgct
    22201 cttttaataa gtccttaatt tctatagcaa cagcatcagc ttttgctttg aaccggtctt
    22261 ttatgattcc cataatgcct tatttttcgg ggattattta ccaatagatt tcaacgctaa
    22321 aattacacaa agccccctgc tgtaacaaaa attaagtatg aaagtttgaa ctttaaatca
    22381 gtgaccctgt aagtttattt ttatttgggt gtgcggcggt aaagccaaaa ggctacccca
    22441 gcaaatacaa tgtttggcag ccacgcagct aataaaggag gaaaattacc cttggtagca
    22501 aacacggttg aaaaacggtc ggacatgata aataatgctg cgataatgat accaagcgcc
    22561 atatgcatac cgctgccacc cctggttttg cgactggcga taacaacacc gatcatcgtc
    22621 aacaataata ctgctgctgg cgtggcactg cgccggtacc gctccacttt taatgtattt
    22681 aaaccttctg ttcccctcag ttcctccttt tcaataaacc ggaccagttc aggagtggta
    22741 agtttatctt tcaggtaatc gtctttacgc agttcagccg ggttcaggtt gaggttaatt
    22801 aaaagtgtat cgatattttt caaccgttcc ccgatactgt ccacatatct ttctgccacc
    22861 ctgaataatt tccattttct gatggaagtg tcccattgca atgtttcagc ccgaaggtta
    22921 taaaccacct tattgtcttt gatcttttcc ataaaaaaac cccgggctgt tttactcgtc
    22981 gtatcataat ccctgatacc aataaaagtg ttggtatcag tacgtaaata aaaacaacgg
    23041 gcacaactgc caaatgctcg gttcttggta ggatcattct tgtctatatg ggtagtctga
    23101 aagtcactcc ggataatatt ggctttgggt atccaatatc ggctccctac ccaaagaagt
    23161 atggccagta aaacaccacc tatgacatat ggcctcaaaa aacggttgta gctggtaccg
    23221 ctggccagta ttgcaatgat ctctgaccgg gtagccatcc ggcttgtaaa aaagatcaca
    23281 gcaataaaaa cgaataatgg gaaaagaagg ctccagatat agggaacaaa ccccaaataa
    23341 tacttgttga tgatctcact ggtggtaaaa tcggccttta caaaatcatc tgttttctcg
    23401 ctgctatcaa cggctactgc aatcaccgtg aacaacaaca tacaaaatat aaatgtgacc
    23461 aggaatttct tcagtatgta ccagtcaagt ttctgcattg gctcaaaagt aactgaaaaa
    23521 ctgttgccgg attgtgaaag caattagaac ttacggatcg caaaaacagt tgcaggtgct
    23581 tataattttt gcttcaatct ttccaccatc tcatttttcc aaatgttaaa attgccctgc
    23641 tgaatatgct tacgggcttc ccccaccagc cagaggtaaa aagcaagatt atgaatactg
    23701 gcaatggtat atcccaaaaa ctctttcgcc ttcatcaaat gcctcaggta agccttgctg
    23761 taaaaattgc tcacttcaca gtcgaaatta tcgtcaattg gagaaaaatc attctcccat
    23821 tttttattat caatattgat gacccccttg ctggtaaaca gcatagcatt tctgccgttc
    23881 cgggtgggca tcacacaatc aaacatgtct acccccattc caatacactc caggatattc
    23941 cacggggtac cgactcccat cagatacctt ggtttatcaa ctggcagatg gtcacaacac
    24001 caatcgcata tttcgtacat aactggttca ggctctccca cactcaatcc accgatagca
    24061 tttccgtttg cttcttttga ggaaatatat tcacatgatt gtttacgcag gtctttaaaa
    24121 gtacctccct gcacaatagg aaacaggttc tgcatataac cgtactttgg ctgcgtagca
    24181 ttgaattgct gaaaacatct gtccagccaa cgatgtgtca tctccatcga tttttttgcg
    24241 taactgaaat cacttccccc gggaggacat tcatcaaagg ccattatgat atcacctccg
    24301 atactacgct ggatatccat cacattctcc ggggtaaaca aatgcttact gccatcaata
    24361 tgggattgaa aagtcactcc ttcttctttt atctttctcg taccggccag tgaaaaaacc
    24421 tggtaacctc cactgtctgt caggataggc ttattccaac cattgaattg gtgcaatccc
    24481 ccggcagctt ccagcacttc taatcccgga cgcaggtata aatggtatgt attaccgaga
    24541 attatctgag ctttcacatc attcaccaac tgctgttgcg ttacagcttt tacactaccg
    24601 accgttccta cgggcataaa gatgggtgtc attatttcac cgtgatcagt tgttattttg
    24661 ccagctcttg ctttggagct gctgtcttta tgttgtaatt gaaattgtaa tgccggcata
    24721 ttggccgcaa gataaaccga aactgttttc cacaacctgt gaaatatatg ctccgcaaca
    24781 acaacagcca gtcattacct tcgccgttat gccaccaatt aactggggag aaattctctt
    24841 gtactttttt attgccgtaa ccgcaataca gcttttttac tatggatggt tcttcagcag
    24901 ggttgctttt ttcaagccta aagccaaagt gcaatcgcaa cagcaccctg tttctgttat
    24961 tatttgctca agggacgagg atgaaaacct ggcccgtaat ctgcccggtg tgctggtaca
    25021 acaatacagc agcagccacg aagtggtggt ggtaaatgat aattcattgg atgattctaa
    25081 atacattttg caggaactga aaaaaacttt caagtctttg catgttgtgg aactgactca
    25141 ggaagcggta catatttccg gtaaaaaata cccattatct attggtatca gagaagcaaa
    25201 gtatgaagta ttgttgctta ctgacgccga ttgtgtgccc gccagcgaac actggataca
    25261 aaaaatgcag gacgggtatg ataagaatat tgaaatagtg ctgggatatg gggcatacca
    25321 taaaagaaaa ggactgctga ataaacttat acgttttgaa acgtttcata cagcacttca
    25381 gtacctgtct tatgctttgg caggaattcc atatatgggt gtaggaagaa acctatcata
    25441 caaaaaagac ctgtttctcc gcagcaaagg tttttcttct atcaatcata ttcccagcgg
    25501 ggatgatgac ctgtttatta ataaggtagc cacaaaaaac aatacagctg ttgttattga
    25561 ccctgatgca atcacccgtt ctattcccaa aaccacctgg ggtagctggt tacgtcagaa
    25621 atccaggcat tatacaactg ccaagtttta taaacccaaa cataaatttt tattgggcct
    25681 ctattttgct acccaattca tttattaccc tttgctggtt actacggcca ttctatacga
    25741 ttggaggttt gcgttggcca ttttcggact tcggctggca ctacaagctt ttgttttgta
    25801 taaatccatg aataaaatgg gagaaaaaga tctttggccc tggttcattt tcttagacat
    25861 gtggatgttc ttttattata tcatctttgc ccccgcactg tggagaaggc cccggaataa
    25921 atggtaaccc cgcaccataa ggattgtaaa agaaacaata tggaaattat tttaaaatac
    25981 ttctcggatt ttactgagat acagctacaa caattcagaa aacttgaagg cttatataaa
    26041 gaatggaatg aaaaaattaa tgtgatttcc cgaaaagata ttgatagttt gtacgaaaag
    26101 catgttttgc attccctgag tattgcagct gcctttgagt ttgaacccgg atccgaaata
    26161 atcgacatcg gaacaggcgg tggttttccc ggcattcccc tggctatttt tttccctgaa
    26221 ttaaaatttc acctggcaga tagtattgcc aaaaaactta aagtagtgga agcggttgcc
    26281 gggagtattg gtttaaaaaa tgtgacaaca caacatacaa gggctgaaga gatcaaaaac
    26341 cggaaattcg attacgctgt atccagagcg gtggcaccat tgaaagattt atggacatgg
    26401 agcagacctt tattacataa taaacccaga acagtagata cgattattaa gccaggactt
    26461 atatgtctca aaggaggtga cctggccaaa gaaattcagg aaagtggtac ccggccacat
    26521 atgatagaaa ttaaagacct gttcccggag gattctttca aagaaaaata cctgctctat
    26581 gtcccccggt aaaaattacc actttgtggt attgttagtc ctgttttaaa tgatgaactt
    26641 taaagaatga gacaaatttc tgttttcata gtagatgata ataaagagct gagaaatgca
    26701 ttggaagaaa tagtctccat gtctgaaggc tataaatgca tagggacagt cggatcagca
    26761 gaagaagctg tcatccagat tcccatctta aaaccagatg tagtgttgat ggatattaat
    26821 cttggaacag aagaaagcgg cattgatgta gtaagggtat taaaaccccg cattcctgat
    26881 actaatttta tgatatgcac cgtatatgag gaagatgaaa aaatatttga agcgttgagt
    26941 gccggtgcca gtggctatat attgaaaaaa acatctcccg ggaaaatgct ggaagccatc
    27001 cgtgaactct atgaaggtgg tgcaccgatg agcagccaga ttgcccgaaa agtagtagca
    27061 gcttttcaac aacagcaaaa tcaatttgcg gtaaatgata gtgagcagct aaacctgcta
    27121 acgaacaggg aaatggaaat actcgaattt ctctctaaag gtttaatgta taaagaaatt
    27181 gcctttcaaa ctttcctcag cccggaaact gtccgtaaac atgtgtacca tatttatgag
    27241 aagttacatg ttacaaaccg ggtagcggct gtcaataaat tctacggaag ataatttttg
    27301 caggtttttg caggaaaaat cagaaaaatg agtaaaaaat accacaaaag tggtattgtc
    27361 tggtcttgaa aagcccaata catttgataa agaaaaaaaa tattctagac cattaaccat
    27421 tagaccaaat cttacccttg agcttaagca agcaaacaag ggccctgatc gaaaaccgtt
    27481 aaaaaaagcc tctgtcaaaa actgaggctt tttccatttt ttcattacca ttttaagtcg
    27541 agcttattat tcttccggtc aatatctgcc attctcattg taggatcgat ttctacaatt
    27601 gaaaaatcag ccagtttccg ggtggtctcc acagtgtatg ttgcatgagt ccatttccag
    27661 gcctcatata cttttcggga aatagctgaa tcctcaaccg gtttctctcc atacatgaga
    27721 ttcatcggta tataatgtaa ttctttacta ccgtccttaa atgtaaattg aaggtcgata
    27781 ggcataggca tcaaaccaac tttattcagg cggacttttg ttttacctcc ctcttcccat
    27841 aaactatcaa tactataatc aatggtcttt acagagttta cccaatactc tttataccag
    27901 tcaagcttca tatcactcac tgtttctgca atccggataa agtcggctgc atttggatgc
    27961 ctgaagcgcc attgcctgta atactccaat aaaattttat cccttaccgg cgcaccggtt
    28021 atataaccca attgctctaa aaatacggcc ccctttgaat aaactgccgc accgtatgca
    28081 gcattggtat taaaatggtc agcatgagtt gttaggggct cttctcttcc gctctttaca
    28141 agagcataat atccccggta agttccatca aatggaaaag cagtactgtt ttcgagaaac
    28201 gcagtaacta aatcctcagc ataggtagta aacccttcat ccatccagcc atacaatgac
    28261 tcgttgttgc ctaaaagacc atgataccag ttgtgcaacc actcatgtag ccaggcaccg
    28321 gggccgatca ataaagtagc catgggatat tccataccac catcgcctcc atgtataaat
    28381 gtatattgcc ggtatggata aacgccgaat tttttttcaa taaaaggcaa tgccttctgt
    28441 gcatcctcta atattttttc ccattccgca ggtgttgcat tggttgactt atataaaaga
    28501 tgaagtgtaa cagtatcatt tacctttctg gttttatgaa catattccgg atcagcagcc
    28561 cacataaaat catgtacatt cggtgcaacg aagcgccatg tcaatttatc accggcaggc
    28621 cggcttactt taacacctgc tgattcatac ccataaccaa tctgattagg attttgtaaa
    28681 tacccggttc caccaagaat atatttttta tcaatagaaa tgctaacgtc ataatcaccc
    28741 catacaccat aaaattcccg acctacatat ggagtaggat gccagccttc ataatcgtat
    28801 tcacatagtt tgggatacca ttgactcatt gagtaacgga cctttgatgt gggattatct
    28861 cttccgcttc ttcttatctg taacggcacc tgtgcttcaa actccatatc aaatacgact
    28921 tttgatttag gcaaaatggg tttatccagt ttcacctcta gaatcgtttc taatatgttg
    28981 aatttttgcg gtcggccatt cattttgaaa gaaattattt tctgaaagcc tatttcttcg
    29041 ggcttcaaat tttgtatacg gtcttttacc cgctgatccc aatccggacc acgaccggca
    29101 atgattgttc cttgccttct gctacgggta tccatcatac taccgggctg aaatgcattg
    29161 aaataaagat gataaaaaac ccgggtcaat gtatccgggg agttattcca atattccagt
    29221 ttttgcttac cggcaaattg attggtctga acattcatat ttatactcat cgtatacttt
    29281 actttctgct gccaccggtc gggttgggcg tcagccttta gcagcacgaa aaaggaacca
    29341 acagataata caaatagttt attcatttaa tacgatttga attggtaaat ttcgtgaaat
    29401 acctgttatg ataaaaacaa aattgattaa tggttaccca tttatggcat atgaaaagga
    29461 gtccttttta cctgctgaaa tgacaaaacg cagccttgaa ttttataaat ggatggataa
    29521 aagaagaaca gtaagagact tttcggataa acctgttccc aaagaaacga ttgagaatat
    29581 tttaatggca gcttccaccg caccgagtgg cgcacacaaa caaccctgga ccttttgtgt
    29641 tgtcagtgat ccgggactaa aaaaacaact ccgcgaagaa gcagaaaaag aagaatctga
    29701 aagttacaac ggcaggatgc cgcaggaatg gcttaatgat ctgcttcccc tgcaaactga
    29761 ctggcgaaaa gaatttttag aaacagcccc ctggcttatc attgttttta aaaaaagcta
    29821 cgaattaaat agtgatggca gtaaaggcaa tgtatattac gccagtgaaa gctgtggact
    29881 agcctgtggt tttttattaa cagctattca tcatgcgggc cttgtagcat taacacatac
    29941 accatcacca atgaactttt tatcaaaatt gctgaacagg ccggagaatg aaaaaccatt
    30001 tttactgata ccggtcggtt atccggcaga tgaatgctgg gtacctgtat taaaaagaaa
    30061 agagttaaat gaaatgagtg tttggctcta atcttttcct gctatgacaa ttactatttc
    30121 acccttcacc tgtttcccat tgaaataatc agctacttca cgaagggatc cccgtttatt
    30181 ttcttcaaac attttggtca gttcccggct tacagaacat tgcctgcttt caccaaaata
    30241 agtaataaat tcttccaggg tttttaccag ccgcagggga gattcataaa agatcaatgt
    30301 tctctcctct tcagccagtt gtttcaataa cgactgcctc ccttttttta aaggcagaaa
    30361 cccttcaaaa gagaaacgat tggaaggaat gccgctattt accaatgcag gtacaaatgc
    30421 tgtagcaccc ggcaaacact ccaccttcac cccgacttta atacattctc tcactaatag
    30481 aaatgcagga tcagaaatac caggagtacc tgcatctgtt accaccgcca tctttttacc
    30541 ctccagcaac tggcttacca aatgatgcaa tactttatgt tcattatgct ggtgataagg
    30601 agaaagaggc ttggtaatct ggtaatgcgt cagcagatgt gaagtagttc gggtgtcttc
    30661 cgccaggatc aggtccactt ctttcaatac ttcgattgcc cggagtgtga tgtcttttaa
    30721 attgcctata ggagtgggaa cgaggtaaag catcaatttg aaaatttgaa aatgtacaaa
    30781 tttgaaaatt gaattcccca aaccgggaat agttttcaaa ttttcgaatt gtcacattgg
    30841 ctaattaact gaaacttcaa agtactccat caccgggttg ctcagtaatt ttttactggc
    30901 ctcttcagct atagctcttg ccttttcagg agactcagca tttacctgca gtgtgatatt
    30961 cttcccaacc cttacatctt ccacaccacc taaaccaaga ttttccaatc ctcccattac
    31021 agctttccct tgcggatcca gcaattcttt cagcggcatt acttttatct gtacagtata
    31081 ggtcatgcta atttattttt gaaaagcaaa gataagagaa cataagccaa cactataacc
    31141 ggaacggaaa gccattttaa taaaactatt gccaaaacgg caataacaat caataagtac
    31201 cggggtaaat tattctgcca actataatct ttgaacttca tcgccattaa cggaatcgta
    31261 gaaatcatga ggtaagaaag aaccaataca ttaccatata aaaaccattt attcaataac
    31321 aaattataga cccacggttc attcacattc cagtaaatta atggaagtga agccacaaac
    31381 agacctactg caggaaccgg cattcctttg aacgaatagg attgactggt atctaaatta
    31441 aatcttgcca gccgccaggc cgctgcacaa ggcagaataa atacaggtat taaccagagc
    31501 aaactaattt caacacctcc ttcttcctgc gcaaagctca ggcgtagaaa ctggtaaata
    31561 atcatggctg gagccacgcc aaaacttacc acatcagcta atgaatccag ttgcttcccg
    31621 aactccgaag tagccttgaa aagccgggct acaaaaccgt cgagaaaatc aactatagcg
    31681 gcaagaccaa tgaacaatga cgtcatccat attttttctg gtatatcaag tagttcggtt
    31741 ccttcagcac tgacattgat taagatgcca ttttgcaacg taaaaacaat ggcaatacaa
    31801 ccaaaaaaaa gattcagcaa ggtgaagaga ttagggattt gcttcatgcc ggcaagataa
    31861 atttattttt tcatgagcga ttctatctct tctgcagtaa tgggaatatt tttcatcaga
    31921 tctttgttac cattcctggt aatccagaag ttattttcaa tacgaatacc catctgttct
    31981 tcctcaatat aaatacccgg ctctatggtg aataccattc cggctttgat gggctctgtt
    32041 ctcgtaccca aatcgtgtac atctactcct aaatgatggg aaataccatg gtatagatat
    32101 tttcgatagg ccctgttttc aggatcttca ttctttacat ccgactttcg caataatccg
    32161 atcttcagga attgttgcgt agcttcttct cctaccttat cagtgtattc aagtatgctg
    32221 atacctggct ttaatatact cttggcataa ttatgcaagt gaagacagga attgtaaact
    32281 gttttctggc gacgggtaaa ttttccattc accggcacgg ttcgggtaag gtcggcacaa
    32341 taaccaccat actcagcacc aaaatccatg agtatcagtt ctccatcttt acattcggca
    32401 ttattactaa catagtgcag tgttcttgct ctgtcaccac tggcaataat gctgccataa
    32461 gcttcgccgg tagccctttg actaaggaat gaatgaaata tctctgcttc gatttcatat
    32521 tccattacac cgggcttaat aaatttcaat aaacggcgga atgtattttc tgtgatatca
    32581 attgcctttt gcattacctc tacttccagt gccgttttaa taccacgaag ctctttcata
    32641 atcttagccg cccgcaggta attgtgcagc gggtaacggc ttttcatttc atcaatgaaa
    32701 cgataatctc ttgacctgac caagcttgct tttcgatcat tttcgttact gtccaggtag
    32761 atattatcag caaggtgaat ccatggttgc agtaaaccat ctgcaacatc caaccataca
    32821 atagttttga taccactgat atcctgtgct tcttttactc ttagtctctt gccatcccat
    32881 ttttccttta attcattggg gcgaaccaat accagtactt cacgatactt tggatcaggg
    32941 ttatccggaa agagaatgat catactatcc tcctgggtga taccgctaag ccagaataaa
    33001 tcactgtttt gtttgtaact aaaaatagca tccccattgc ttggcacttc gtcattactt
    33061 acaaaaatgg ctatggaatt tttttgcatt tgacgggcaa atcgttccct gttttgaaca
    33121 aagatttccg ggtttaatgg cagatatttc atggtagcaa tttgaaaagt ttagaagaat
    33181 caaatcaata attatggcta aagatagcaa gctatactat caggcgattg attttcatca
    33241 caaaaccacg aaacatggat aaaattcaaa gatttccaag tattacagct ttttatttta
    33301 ctttctttaa cccaaagcta tgaaaatgaa taagaagcga tttttttcgg taatatatta
    33361 agccctttct aatgtgtcat actaacactt gtttgtgata gtattagtat agatattacc
    33421 tttgtagctt caacaaattt gtttgctaaa tatgtcagta cctaccatcc cttttccgac
    33481 ccaaaattta tttaaatcaa gccttaaaaa tacagaatcc attcattatc aatcttctcc
    33541 agaggagctg gtacaagata ctttgcgaat tggagaaggt gtattgaatg ataccggtgc
    33601 ccttgttatt aaaaccgggg agtttacggg tcggtcccca agagacaaat tcacggtaaa
    33661 agatgagatt actactgaca ctgtgcattg gaatgaattc aatattccca tggaaacaaa
    33721 aaactttgat ataattttcg aaaaagtcag tgcgtattta aactcactta atgaaatatg
    33781 ggtaagagac tgttatgcct gtgccgaccc ccgctaccgg ttaaatatcc gggttattaa
    33841 tgaaaagccc tggaacaatc tttttgccta tacgatgttc cttcgtccaa ctgaaaagga
    33901 gttggagagt tttgaacccg attggcatgt tatttcagtc ccggggttaa ggcttgatcc
    33961 ggcagcatgt ggaacccgtc agcataacgc ttccgttgtt tccttcaaac ataaaatgat
    34021 attgattgcc ggatcgggct atacgggtga aaccaagaaa ggtattttca ccattttaaa
    34081 ttatatttta cctcaagaaa agaatgtgct cagtatgcat tgcagtgcca atatgggtac
    34141 tgatggtgat accgctatat tctttggatt gagtggcact ggcaaaacaa ctttaagtgc
    34201 ggaccctaat cgtaaattga tcggcgatga tgaacatgcc tggactgatg ataatatctt
    34261 caactttgaa gggggctgtt atgcaaaaac aattcacctg acagaagaga aggaacctga
    34321 aatttataat gcaattcgcc ccggtgccct ggtagaaaat gttactttct atcccggcac
    34381 caacaagata aattttagtg acgcttcggt tacagaaaat acaagggttt cttatcccct
    34441 tcattttatc agtaatgcac aggagccttc cataggcaac ctacctaaaa atattttctt
    34501 ccttacgtgt gacgcatttg gtgtattacc tcctatttcc aaattaacac cgggccaggc
    34561 aatgtatcaa ttcatttctg gttacactgc caaagtcgct ggtacagaag ctggtataac
    34621 agagccaaaa tccactttca gcgcttgttt tggggcacct tttctgccac tgcatcccgg
    34681 taaatatgca gcaatgctgg gcaagaaaat gcaggaaaat aaagtgaatg tgtggatgat
    34741 caatacaggt tggaccggcg gtccatatgg tgtaggcagc cggatgaaac tgaaatacac
    34801 aagggcaatg atcactgctg cacttgaagg taaattgaat gagacagaat ttgataccga
    34861 cccgatcttt ggcatggcta tccctaaaca atgtcctgat gttccttcag aagtgctgca
    34921 ccccagaaat acatgggttg ataaaaatgc ttacgacgaa aaggcaaaat acctggcagg
    34981 cctgtttatt aagaatttta caaaatatgc tgatggggta acgccagaaa tactggctgc
    35041 agcaccaaaa gcgtaagata tcaactttgg acggtttcct ggaatgcccg ccccgcctta
    35101 gcgagacggg tttttctttt atttaacgcc ctgccttgca gctaataaaa tttttgatac
    35161 gacattacgt tgtgattgtt ctatgcaaaa ttttttaaaa tatttcctgc tttttctttt
    35221 gcccataacc gcatctgcgc agctgcccga tgaagagtta ctggaatgga gttcggaacg
    35281 taaacttagc tggagtgatt ataagggtaa acctaaccct ttaagtgatg cagcagcctc
    35341 cactactacc tatttaagta tagaatataa tatttcaagc agcagttttg gttacaaaat
    35401 aaagtcccgc ttctccaaaa accggtcctg gggtttgcat aaatcagatt acatcctcag
    35461 tcatgaacag gggcattttg acattgctga aatatttgcc aggaaactgc ataaaagaat
    35521 gggtgagtat tcctttaaca aacgaacata tcagcaagac cttaagaaaa tctatgaaga
    35581 agtgacagat gaaaaagagc aaatgcaaaa tgactatgac agggaaacaa gacacagtat
    35641 taacaaagaa aagcaggcag aatggttgaa aaaaattgag aggatgctgg aagatctaaa
    35701 agtttaccat aattacaaat cactctgata tcaaattaaa atagtccata cagctccgca
    35761 tctactttac ggatgatctc gccaaagtct tcatcccttt cggcaaattt attcttgtca
    35821 acatcaatga ccagtagttt accttcttta tactccccaa tccatttgtt atagaactca
    35881 ttcagcttct tcaggtaatc gaggcggata ttttcttcgt attccctgcc tcttttttga
    35941 atttgtccaa caagagtggg tacagaagca ttcaggtata tcagcaggtc gggtggctga
    36001 accagcgttt ttaaagtctg gaaaaaatga aagtaattat caaaatctct tttactcatc
    36061 aggcccatct catgcaggtt gggtgcaaag atgttggcat cttcataaat cgttctgtcc
    36121 tggatcaccg tctctgttcc cttttgaatt tctactaact ggcgaaggcg gctgttcaaa
    36181 aagaatatct gcaggttgaa actccagcgg ggcatatcct cataaaaatc catgaggtag
    36241 ggattgtgat ccacatcttc gaaatggggt atccatttgt aatgcttgct cagcatttct
    36301 gtcaatgtgg ttttaccggc accgatattt cctgctacgg ctatgtgttt tggtttcttt
    36361 gcttttgcca taccccatgt ccgccggccg gcggagaatt tttaatttca aaaatgaata
    36421 ggcgttaaaa gtattgaatt ctcactattc aaagacccct attaaaaata aatcagaaat
    36481 attttaaccc aatcgcctcc ctcaccagtt ccatcgtggc tttagcactt ttccgggcct
    36541 ttgcggctcc tttttccatt acttcctgta aatacctttc gtcattacgg atcgattcgg
    36601 ccttttctct gatcggactt ataaaattca ccatatcctc cgccagttgc tttttcatat
    36661 cgccataccg gatggtgcat tggttatagt cctcttcaaa ctttttaatc acctcttctg
    36721 aactcaccag gttcattaac agaaagatat tctcaatata atcaggtttg ggactgccgg
    36781 gtactgttgg cccactatca gtttttgctt tcatcacttt cttgcggatc agttcatcat
    36841 catccgatag gtataaagta gcatattggt tttcactctt gctcatttta ccggctccgt
    36901 ccaggctggg cacttttacc agttcactgc cataattgaa tgcctgcggt tcgggaaaaa
    36961 cttctccata tcgatggttg aaccggttag caaaatttct tgccatttcc aggtgttgct
    37021 cctggtcttt gccaacaggc acatacaatg cacggtgaag aattatatct gctgtttgca
    37081 gtaccgggta agtaagtaat ccggcattta cattatccgg gtgctgccta actttatctt
    37141 taaaagtgac tgttttttcc agttcccctt tatatgcaag catattcagg tacaggtaaa
    37201 gttctgcagt ttcataaata tgactttggc aatagagtgc cactttatcg ggatccagtc
    37261 cgcaggcaat attctctgct aatacccggt gcacatttgc ttttaattct tttgtatcgg
    37321 ggtgggtggt caatgaatga agatctgcta ccataaaata acattcaaaa tcctcctgca
    37381 tccgtacata gtttcttacg gctccaaaat aattacccaa gtgtaaaaaa ccggtaggcc
    37441 ggatgccgct cattactatt tctttccttt tttccatgag ctggcgaaga tagtaattag
    37501 tcaggagtct gtataccgga gccggagacg ttctgattgc aaaactcatc actcccgact
    37561 aacgactcct tactatcttt gcaatatgga agtgaacgat aaactggtgg ataaactggc
    37621 ccatctttcc cgccttcagt ttaatgaatc agaaaaagct gaaattaaaa atgacctgca
    37681 acggatgatt ggttttgtag aaaagctcaa tgaactgaaa cttgatggta ttgagccttt
    37741 attgcacatg agtgaagaag tgaatgtatt gagagaagat gaaataaaag gatcagttag
    37801 ccgggaagaa gcattaaaaa atgccccgct gcatgatgaa caatttttca aggttccgaa
    37861 agtgataaaa aaataatatg gcacagggga tcattcatct ggagaatatt cagaaaagtt
    37921 atttcatggg caagaacgaa ttgcaggttc ttaaaggcgt atcactggac atatttaaaa
    37981 atgaatatgt agcactgatg ggaccttccg gttccggaaa aagcaccctg atgaatatta
    38041 taggatgcct ggatacacca acagcaggca ggtatatatt gaacgataat gatgtgagtg
    38101 aaatggaaga caatgcactg gctgaagtac gaaataaaga aatcgggttc gttttccagc
    38161 aattcaatct gctgccacgc ctcactgcag ctgaaaatgt tgctctgccg ttggtctatg
    38221 caggcatgaa ccgtaaactg agaaccgaaa aagctatgca ggtactggag atggtaaacc
    38281 ttaccgacag aagtcatcat aaacccaatg aactttccgg tggacaatgc cagcgggtag
    38341 ccattgcaag agcccttgtt aataatcctt ccataatcct ggctgacgaa cccaccggta
    38401 acctggacac caaaacttct tatgagatca tggagatttt taatacgata catacaggag
    38461 gaaatacgat tgtcctggta acacatgaag aagacatagc taatcatgcc catcgcatcg
    38521 tgagactccg ggatggaata attgaaagtg ataaaaaaaa tgagaatgtt ggtgaatatt
    38581 ctcaccactg actgtgaaaa actttatctt ttcccgttcc ccttaaaacc tttctttgat
    38641 aaaaattgtt ctctgtgaat atcccgcaat aaaatgactt ttaaaattta taccaaaaca
    38701 ggagataaag gaactacttc attgattggt ggaacaaaag ttcccaaatc acatctccgc
    38761 attgaagcat atggaacagt agatgagctc aactcctata ttggcctgtg cagggatata
    38821 ctgaccgatg agaacagccg tacagtttta caggaagtac aggatcgcct attcacaatt
    38881 ggctcctcct tagcttgtga ccccatcaaa gagccaaaga tgaggatacc cgatctgaaa
    38941 gaaaaagatg tagagctttt ggaaaaagaa atggataaat tgaatgaatc tattccagaa
    39001 atgaaaagtt ttatactgcc cggcggtcac accacagttt ctcatttaca cattgcccgt
    39061 tgtatttgcc gcagggcaga acgttgttgc gtccggttgg aattagaaag ccttgaagtg
    39121 gaacaagtaa tccttaaata cctcaaccgc cttagtgatt atttatttgt attaagccgc
    39181 tacaccggtc accagttact tgtgggagaa ataccctgga agccaagagt ttaactttat
    39241 tgctgtttat tattgctgaa attagtacca aattttgcta ttttaccaat taattattaa
    39301 cctgttcggc atattaatag cacccggacg tttaatgtat ctgtaaacaa ttaataaatg
    39361 cggctacaac taatttcagc agttacttca ctaacatatc tgcttagcag ttgtcagccc
    39421 agtcccgttg acccaccacc caatggacca gtggtattga gagataccac tatcaataat
    39481 attttatacg gagctgatcc tgcccagaga ttagacctcg gtttaccggc taacagaact
    39541 tctaataccc agctggtggt tattattcat ggcgggggtt gggccaccgg agataaaaat
    39601 gaattaacat gggcccttaa tggattaaaa caaaggggtt ttgccgttgc caatattaat
    39661 taccgtttaa ctttaaatac agcagataac tataaaatgc agttggatga tattgacagt
    39721 gctgttcagt tcactttgcg ccagtcatct gttcacactt tcaatggcca gaaattatat
    39781 atagtgggtc atagtgccgg tggtcatctt tctctctgtt atgcgtatac aagaaataca
    39841 aatggtaaag taaaagcagc aggttcgctg gcaggcccca cagacctttt tgctatggct
    39901 tattacagtt ttaatatcta taacccatta ctcaatcctt acctcggtat gccgttgatt
    39961 cctgtaacac cggtttcaga acaacggtat aaaagttgca gcccccaata ccaggctaca
    40021 acaactgctc ccgccactat tttcttacat ggcgaacttg atataattgt aaaccctgac
    40081 cagagcagtg gcatgtatgc taaacttgga acccttggag ttgacagaaa actggtactc
    40141 tatccactca cttttcacga ctggtggact gatggaacta aagtagctaa cacactggat
    40201 gagttaaaaa catggttcaa taaccatccc tgattaaaat ttctttactg cacaatctta
    40261 ccatctttca tatgcaaaac ccggtcactt agctgtgcca gttcttcatt atgtgtaaca
    40321 atcagaaatg tttgactgaa ttgttccctt aaatcaaaaa acaaccggtg caggtctttg
    40381 gcattggctg aatcgagatt accggtgggt tcatcagcaa aaatgatatc aggtttgtta
    40441 atcaatgctc ttgctacagc cacccgttgt tgttcacccc cggacagttg atttggttta
    40501 ttatccattc tttgtgacag ccccagcata gttaataatt tttctgcttc cgttttaact
    40561 tccggtttct ttctgccagc cagccaggca ggtatagaaa cattttctaa tgcactaaac
    40621 tcgggcagca aatgatgaaa ctgaaaaaca aagccgatat gcttatttcg gaaagcggcc
    40681 aactttttgc cacttaatga attgatgata acattattca tactcacttc acccatgtct
    40741 gctttatcca gggtgccaag gatatgcaat aaagtgcttt tcccggaacc ggaagggcca
    40801 acaatggcaa ctacttcccc cctccctatt tccacatcaa cacccttcag aacttcaact
    40861 tttccgtatc gtttataaat ttttttgccg gttatcatca gtcaaatatc gcaaatagag
    40921 ggcagcagca aaaacaatgg tttcggatag acctgaaatc ttaagaaatc cttcaaaacc
    40981 tggtgaacaa ctaaggattt ggaaaattcg ttaatacaac tacatttgcg gaaaattagg
    41041 gggtatccga aaggaagttg gatgcccaaa accttattca aaaacaaaaa ccctgagaag
    41101 atgttttgga ctttagaact agcctcgtac cttgaagacg caccctggcc cgctaccaaa
    41161 gatgagctga tagattattc tattcgctct ggtgccccca ttgaagtggt agaaaacctg
    41221 caggaattag aagatgaagg tgatgtatat gagactatcg aagatatctg gcccgattat
    41281 cctacccagg atgacttctt gtttaatgag gacgagtatt aggaaaatga tgagcctaca
    41341 tatttataaa taaagcctcc gttttggagg ctttattatt atgaaaatta agaataccgg
    41401 tgaaacactg caatcatcat ccctagtgtc acaccggtta ctgcatattc cttttctgca
    41461 ttttacacag ctactttcgc cattcgctta ctctgaaaca cccaacttct gcatcacttc
    41521 gttactcaca cctgttgtgg aatatccccc gtcatgaaac aggttttgca tcgtcaccat
    41581 tcttgttaaa tcagaaaaca gggtaatgca atagttggca cagtcttcag ctgtagcatt
    41641 gcccagaggc gcaattgaat ttgcaaaatc aaagaaatca ccaaatcctt ttatcccggt
    41701 gccagctgtt gtctttgttg gagactgaga gactgtatta atgcgaactt tcttatccac
    41761 cccataatga taaccaaagc tgcgggcaat agattccagc attgctttaa tatcagccat
    41821 atcggtataa aaaggatacg tacgctgagc agccatatag gttagtgcca ccacactacc
    41881 ccactcattt attgcatcca actttttcgc gactgccagc attttatgaa atgacatggc
    41941 agagacatca atccccttca taaaataatc atagttagat tctgtataag gtatcttttt
    42001 cctgatatta acactcattc caattgaatg gagcacaaag tcaatttttc cgcccaatac
    42061 ttcctgtgat ttagtgaaca gggtggtcag ttcatctaca ttcgtggcat ccgcaggaat
    42121 gatttctgat cttgtttttt cagccagttt attgatttca cccatccgca tggcgatggg
    42181 cgcatttgtt aatacaatag acgcaccttc ttcatacgct ttttcagcta ctttccaggc
    42241 aatggaattt tcatccagcg caccagtaat gattcctctt tttcctttta aaagattata
    42301 agccatagca tttaatttta atgcggtaaa aataaggaga tatttctaag ccccgcgaaa
    42361 gacgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttaa ttttaatgcg
    42481 gtaaaaataa ggagatattt ctaagccccg cgaaagacga attttatgat aaaaaccagt
    42541 aaaaaaatat agacaaaaat aaatgtaaac acaaagagca aaaacagtaa gaatttccgg
    42601 attgattcct atggtttcat accctgttgc aggtatttgt aaaagacctg cactggtttc
    42661 agtgacagaa gaataatccc caatatcagt tttgctttta gtttcaattg catcagctat
    42721 ttcaatgata aaaagaaagc tgctgcaata tatacactct tagttttacc cgggtggccg
    42781 gcaataaatt attgcccgca attggatacc tctaaccaat actgcgctac atttgtacaa
    42841 gaactgattt tcttaaattt ttaagttcga cattatggat atcagaacaa tcataaccgg
    42901 aaccggctgt tacatccccc ccatcacaaa aacaaacaag gagtttgtca cacaggattt
    42961 ttatggagaa gatggaaagc tgattactac ccccacacag gatattgtag agaagttcgg
    43021 ggaaataacg ggtattgaag aaaggcgcta tgcagctgaa gacatgaatt cgtccgatat
    43081 ggctaccatt gcagcgagac tagctattga agatagtggt attaaccccg aggatttaga
    43141 ccagatcatt gttgcccaaa attttggcaa cgttattaaa cataccattc aaacagatgt
    43201 attgccagca ttagcctccc gggtaaaaca tgacctcggt ataaaaaatc ccaattgtgt
    43261 ggcctatgat attttgtttg gctgcccggg ttgggtacag ggggtaatac aggcagacac
    43321 ctatttcaaa gccggtgttg ccaaaaaggc actggtagtg ggtacagaaa cattaagccg
    43381 tgtaattgat atctatgacc gtgacagtat gatcttttct gatggtgccg gagcaaccgt
    43441 tttagaagca aggcctgccg gatcaaccaa tagtggaatt ctttcctgga gtgtgcaaag
    43501 tcattgcctg gatgaagcct attatttatt cctcggcaaa agcaatcttc ccggctcaga
    43561 cccccgcata cgttatataa aaatgaaagg aagaaaagtg tatgaatatg caatgctgca
    43621 tgtgccggca gccatgaaag attgtttgga taaagccggc atctctattc aagaagtaaa
    43681 aaaagtattc atacaccagg ccaatgaaaa aatggatgag ggcattatac gccgcctcta
    43741 caagttatat gggatcaggg aaatcccgga acatgtcatg ccaatgagta tccataaact
    43801 tggcaacagt tctgtggcaa ctattcctac tttattcgat atggtcaaaa aaggattttg
    43861 ggataatcat gacctccggg aaggtgacat agttttattt gcttctgttg gtgccggtat
    43921 gaatatcaat gctatctgtt accggatgta atacctggca agaaagcact tcctcccttt
    43981 ccattatctt cgcagctatt aaaagaagat acagaacatg acgatttctt acaaatggct
    44041 gagtgaatac ctgcccgtaa ctgtcgaccc ggaaaggctg agccgaatcc taacctcgat
    44101 cggcctggaa gtggaaagta tgaataaatt tgaagaagta aaaggcgggt taaagggttt
    44161 ggtgattggg gaagtagtaa gtactgaaaa acacccaaac gcagataaac ttacaattac
    44221 caacgtaaat ataggacgca cagatccgtt acaaatcgta tgcggtgctc cgaatgtagc
    44281 tgccgggcaa aaagtagttg tagctactgc cggcacaacc atttacccat caaccggcga
    44341 tccgttaacc atgaaagtgg caacaatccg aaatgtggaa agtcatggta tgatttgcgc
    44401 agaagatgaa atcggtttag gaacttctca tgcaggcata atggtattac cgggtgaagt
    44461 aactgtgggc accccagccg ctgaatattt ccagccctat aacgatattg tttatgaaat
    44521 cggccttact cccaaccgta tggatgccat gagtcattgg ggggtggcaa gagatgtttg
    44581 tgcttattta agtcatcatg acaaaaaaga tataaagcct aaactgccga ttaccaatag
    44641 cttcaaaatt gataatacat cttttcttat tgatgtaaaa gttgaaaatg ctaccgcatg
    44701 tccccgttat tccggtataa gtatcgataa cgtaactatc aaagaatcgc caaactggct
    44761 gcaacaaaaa ttaaaagcga tcggactaag gcctattaat aatatagttg acattactaa
    44821 ctacattcaa catgaaacag gccagcccct acatgctttt gatgcagatg ctatctatgg
    44881 aagaaaagtt attgtgaaaa atctgccagc aggtaccagg ttcgttaccc ttgatgaaaa
    44941 agaaagaata ctgagcgctg aagatctgat gatctgtgac gaaaaagaag gcatgtgcat
    45001 tgccggtgta tttggtggtt tacacagcgg ggtaacagag aaaacaaaaa ctatttttct
    45061 tgaaagtgct ttttttgatg gcattacact gcgcaaaact tctttccgcc atggattaag
    45121 gactgatgcc gccagcaggt ttgaaaaagg aacggatatt tctgcaacag ttaatgtatt
    45181 gaaaagagct gccaacctga taaaggaaat ctgcaatgga gaaatcacct ctgaaattgt
    45241 tgacatttat ccaaaaaaga aagagaagac cgaaattgcc atcaaatggc attacattaa
    45301 aaaactgagt ggcaagaatt atcacccgga tgcagtgaaa gatattctta acagccttgg
    45361 ttttgaaatt atgaaggaag gaattgacga attgagagta gcagtccctt atcataagcc
    45421 cgatatcact ttacctgccg atatcgttga ggagatagta agaatcgacg gccttgacaa
    45481 tattgatata cccgaagcaa ttaccatcac ccctgctgtt gaagagaatt atgccaaaga
    45541 agtataccgg gaaaaaacag ccaattacct ggtggggctt ggcttcaatg aaatgatgac
    45601 taattccatt accaatgctg cttacttctc cgaagaagaa ttgcaaacca tggtaacgat
    45661 gaagaacagc ctgagtgcag agctgaatat actacgcaat tcactttttg aaacagcact
    45721 ggaagtagtg gctcataacc tgaaccacaa gaataattcg cttcgcctgt ttgagtttgg
    45781 aaaagcttac agttcatccg gaccgggtag gtataatgaa agcgaaaaat tatgtattgt
    45841 tatttccggt aataaaaacg aagatagctg gaaacaaaaa agtgtttcag ctgattttta
    45901 tttattaaaa ggaacagtgg atgctacgct gcaatcgctg ggcgttcttc ccgtttccac
    45961 agagaaatta cttgccccaa aactggataa tcatattgta tataagtaca ataatcaaat
    46021 tatcgccggg gcaggtgaag ttaaaaaaac catcctggat aaatttggca tcaaacaacc
    46081 agtatttctt gccgaactta actggcaatt cttgtctgaa caagcatcaa cacaaacaac
    46141 tacaatcaaa gaaatatcca ggtatccggc agttcagcga gacattgcta tgatcgtttc
    46201 aaaggaactg gtatgggagg aagtgcatat gactgtgcaa aagattaagc ttgataaact
    46261 acaggacatt aaactctttg atatattcga aagtgaaaaa ttaggggccg gtaaaaaatc
    46321 aatggccgtt aattttactt tccaggatga aggcaagaca ctgactgata aagaaattga
    46381 tggctggatg agtaaaataa tgaccagcct ggaaaaagaa ttacaggctg aaatcagaaa
    46441 atgagtaaca cagaacatca tcttaaacga atacaggata agcttcagca attgctgaaa
    46501 cagtatgctg ctttgcaaaa agaaaacagc cgcttaattc aggagttggc atcagcagaa
    46561 caaaaaatag aagcttatca aaaaaatact gacgaattaa aacagcaagt gagtgttttg
    46621 aaactaagta ccggagaaat gagtgaggcg gacaaaaaag agtttgaaaa gagaattaat
    46681 ggatacctga aagagattga ccgttgtatt gctttattgg gggagtagct tacactcttt
    46741 ttactaaatt cattttcatt tcattctcgc cctgggaaag ttccagtgta taccggccat
    46801 atggcaaatc atttaaacct ccccatatca attcttccct gtcctttgca atgttttttt
    46861 ccatggttcg gcaaatactg ccggtgtcat cctttaaaac accacgaagg caattacctt
    46921 tgggagtatt gagttgaaat tgtaatacat caataaaaac tgatgattta actttagcaa
    46981 acataacggc aaacgttttg gttcgtaact cgtttcccac agtggacctt aacctctttg
    47041 ttttcaattg agtagccgga tgcttttata aagtatctaa tgtagtaaat gaaatcagaa
    47101 aatgcaagca atatgaatga attaattcct atttccgccc tgatcggcga cagaacatac
    47161 cgtatcaaga ttgatccgaa agatgaagag gtggtaagaa ataccctcaa aacaattaat
    47221 gacaagatta ttgaatttaa aaccctgttt gccggcaagg atatgcagga ctatatcgcc
    47281 atggtaatga tttggtatgc gactgaacag caggccggaa aatcggctct tctggaaaaa
    47341 gtaaacctgg gagaaaaact tgattcaata gaaaaattgc tggacaatca tattaatcaa
    47401 taaataagcc ggcatttaac cggcttatca tcagtattaa tctctttact ggttaccgca
    47461 ctcttcttta gggaactgga acaaatccct ttttaccttc aattgcttcc ccaaccttag
    47521 tttccagctc ctcgcccctg atatttttag ctatgatttt cccctccgga tcgagcagca
    47581 agttttgagg aatggctttg atcccatatt gctttgcaac ttcattatcc cagaatttca
    47641 gatcactaac ctgtgtccag tccaatttgt catcatgaat agctttcatc catttttctt
    47701 tttgtcccgg cctgtctaaa gaaacgccga tgatatgaaa attcttgtcc ttatacttat
    47761 tgaacacttt taccacattc ggattttccg tacggcaggg gccgcaccag ctggcccaga
    47821 agtcaatcaa taaataccgt cccttaaaag atgccaaaga aacaggatta cccaacgtat
    47881 cattttgggt aaagtccatt gccattttac ctataccagt tttcttagct atctcaagat
    47941 tctccttaaa ttcaatggca gagggatagt ttttattagt accggataat gaattaaaca
    48001 ccggctctac tttatcagcg tcaatatccc aaccagcata ttgctgcagc gcatacatgg
    48061 ccaaaggaga agacgggttc ttttttacat attcaccgta tactttttcg cttttttctt
    48121 tatcaatagc atctatctga tcctccactt ttgattgatt ctccctgtct tttgctttac
    48181 catattcggt atactttgca atcaatggct tcattcgttc ttcaaacggc ttttcctgtt
    48241 ctttcaattt tacaaactcg gaatgagccg cggagccttt taccttaaca ttactgaatg
    48301 aatccacaga tgtgatcttt atttttccgg gctctaaaaa gacagccact atatccctgc
    48361 cggaaactgt agctatttta ttaccatcat caccagacct gtattttacc ctcaatcttc
    48421 ccaacaccgg ctcagcaatt tctcccgcaa aggaaaactt tccatctttt gtttgaagac
    48481 tatcggtttt ccattcacca ttggtgcggt attgaaggaa aacccagtct ggtatatatt
    48541 tcaggttttt aactttcccc gcaaggctaa agggtttact tttatcctgc gctaaggcaa
    48601 aaaatggtaa tagaccaatg agaaggatta tttttttcat gtactttttt tcaggccagc
    48661 aatttacaga aatgaacatc ctttaccagc gttaaaaatg ccgaaatcct taaaatctta
    48721 cacaaaaggc aacaaaatac ccgatttcat ccgttaagtc ctgttttgta tcttcgctaa
    48781 ttacataaac ttctgtattc ctgacagatg caggtatgaa gtttagatta aacaatagca
    48841 ttgtgcaaca attaaacaaa aaaattaatg gatccgaata tactatccgt catcatcggc
    48901 gcagctgccc ttattgtggg catcatactg ggtaaattcc tttttgcaaa aaatacccgt
    48961 aaacaaatag aagaagcaga actacaatcg cagaccatcc tcaaggaagc tgaattacga
    49021 gctgaaacca tccggaaaga aaaagagctg gaagcgaagg agaagtttgt gcagatgaaa
    49081 tctgcccacg acagggaaat actcgacaag aacaaaaaac taaatgatac ggaaaaccgg
    49141 atccgtcaga aagaacaaac cataaaccag aaagaaggca accttgataa acaaataaaa
    49201 gaaaacgaag ccatcaagga aaacctgaac cgccagattg aactggttaa tatcaaaaga
    49261 actgaactgg aaaaacacca ggaagagcat atacgccgtt tggaaaaaat atccaacctt
    49321 tctgctgaag atgcaaaagc ccagttgatc gaaagtctga aaaacgaagc ccattcaaag
    49381 gcattggcct tgcaacagga aataatagag gatgccaaac aaaaagccgg aaaggaagcc
    49441 cgtaaaatcg ttatccaaac aatacaaaga accgctgcag agcaggctat tgaaaactct
    49501 attactgtat tcaacctgga aagtgatgag ataaaaggcc agatcattgg ccgggaaggt
    49561 cgaaatatcc gtgccattga agcagctacc ggtgttgacc tgatagtgga tgatactcct
    49621 gaagccattc tattatcaag tttcgatcct ctacgccggg aaattgcccg tttgagttta
    49681 caaaggctgg tggctgatgg tcgtatccat cctgccagga tcgaagaagt ggtggaaaaa
    49741 accaaaaaac aaatagaaga acaagtgatg gaaattggcg agagaacggt tattgaattg
    49801 ggcattcatg gactacataa ggagctggtg agaatggttg gcagaatgag attccgctcc
    49861 tcttatggtc aaaacctgct gatgcatagc cgcgagacag ctaacctttg cgccaccatg
    49921 gcggctgaac tagggttgaa cgctaaactg gccaaaaggg ccggcctgct tcacgatata
    49981 ggaaaagtac ctgatgaaga aagtgagttg agccacgccc tgcttggagc taaactggcc
    50041 gaaaaatatg gtgaaaaccc tgcagttgtt aatgccatcg gagctcacca cgacgaaatg
    50101 gaaatgcaat ttgtgatatc tccaatcgta caggcctgtg atgccatcag tggcgcccgg
    50161 cccggagcca gaagagaaat catgcagcag tatctgcaac gtatcaaaga ccttgaaaac
    50221 ctggccatgg cctataatgg tgtagaaaaa gcttatgcaa tccaggcagg ccgggaattg
    50281 agggtaattg tagaggctga taaagtaaca gaccaggaat ctgataaact ttccttcgaa
    50341 attgctcaaa aaatacaaac cgaaatgaca ttcccggggc agataaaagt gacagtgata
    50401 cgggagaaaa gggcggtgaa tgtagcgaga taaaagttat taggtattgg gaattaggaa
    50461 tttaggcagc aaagaaaaaa agtctgattt gaagcctaat aaaccaatca actaattaac
    50521 taattaacaa aaaactcctt acctttgccc tccctaaaaa tcaggattat gaacgctata
    50581 gcatatgtac atgagcaatt gtctggcaaa aaggaatttc cagcctttaa agcaggcgat
    50641 aatattactg tgaactacag gattatagaa ggtaataaag aaaggatcca gtcttttaaa
    50701 ggagatgtga tcaaaaagca gggaaatggt gctacagcca gttttactgt tcgtaaaata
    50761 tctgatggtg ttggagtaga aagggtattc ccttttttct caccaaacat tgaatctatt
    50821 gaagtgaaca aaacaggaca tattcgtcgt gccaaattgt tctaccagcg tacacgtagc
    50881 ggcaagagtg caaggattcg tgaaaaacgc caggctgttg ccgaagttgt caaataaatt
    50941 aatgaagtat atagaaagcc ccgtcttatt gccggggctt ttttatgcac ggcatttttc
    51001 taattaatat tcctttattt ttaagtactt agtatctttg actcctaata atttcagact
    51061 atgatacaac ctacattact gggaatgctg ggcacaaatg aaatcatcat catcctggtc
    51121 attgttttac tgttatttgg tggccgcaaa atccctgaac ttatgcgtgg tctgggtaaa
    51181 ggcgttcgtg agtttaatga tgcaaaaagc aatgtgaaga aagaaattga agaaagtgcc
    51241 agtgatgtaa aaaattccgt taaggactaa tctttatttc ttacacctat tttctctaac
    51301 taaaaagcac cagcttttgt ttgaattcac ttccattgag caattccatg cccaattaca
    51361 ggaaggaaaa gtgacttgtt tgcaggtagt tcaatattac ctgaaacaaa tccattccac
    51421 ccggcatctc aatgcgttta tagaagtata tgcggacgaa gctttacaaa gagcaaaatt
    51481 gctagatgaa aaaagactag ctggtcaaac tattggtaag cttcatggtg ttgttatagg
    51541 aataaaagat gttatttgtt acaaagacca taaggtctct gcatcttcta atattcttaa
    51601 agattttaca tctatctatt cagcaacggc agtagaaaga ctgctaagcg aagaagcaat
    51661 cattattggt aatctcaatt gcgatgaatt tgcgatgggc tccaccaatg aaaactcatc
    51721 gtatggcaat gtgctgaatg cattggatga aacaagagtt cccggtggct cctccggtgg
    51781 ttccgcagtt gctgttcagg ctggcctttg tatgctttca ttaggcagtg atacaggtgg
    51841 ttctgtccgc caacctgctg atttctgcgg gatcattgga ctaaaaccaa cctatggaag
    51901 aatttcccgg tatgggttaa tagcgtacgc ttcttcattt gaccagatcg gaatttttgg
    51961 caataatgtt cctgatgtag cagtattgct cgaagtaatg gccggcgccg acgagtatga
    52021 cagcaccgtt tcgcatcggg aagttccggc ttattcctca caattacccg gcaataaaaa
    52081 attccgcttc gcttatttcg atgaagcatt gaatcatcca agcctggata ttgaaattgc
    52141 taattccatc aagaataaga ttgaacaatt aaaagaagat ggccatgaag tggctgctat
    52201 tgagtttgat cttcttgatt atatcgttcc ggcttattat gtactaacca ccgctgaggc
    52261 ttcttctaat ctttcaagat atgatggcat aaaatatgga caccggacgg caaagaaaga
    52321 tatagatctg accgaatttt acaaactaac aaggtctgaa gcatttggga aagaagtaaa
    52381 acgacgtatc atgcttggca catttgttct tagtgccggc tattacgatg cttactttac
    52441 ccgtgcccaa aaagtaaggc agcttctatg tgataaaaca gcggctattt tcaaatttta
    52501 tgattttatt atcatgccta cttcccctgt tacagccttc aaggccgggg agaaaatgga
    52561 tgatccaata gctatgtatc tcgctgatat ctatactgta atggccaacc tagtgggttg
    52621 tcctgctatt tcgattcctc ttttcaaaca cagtaatggc atgccctttg gccttcaggt
    52681 tatggcaaac cggttcgatg aggtatcttt actgcagatt tcaagtatgt taaagccctg
    52741 aactgatcgc ccattgacgg tcaaaaactg aagctttttt caaatctata aagttgacat
    52801 ttttaaaaag aatattaccc tttacgattg gcttattatt gcttttatca gccagggcag
    52861 gtaaaaacca acaggcccct aagaatgaat tttcaggcat cataaataaa gatgtaaata
    52921 aagactcact cattcaaccg acatcaattc ttaaagatcc aaagcttggc ttcaaaaatc
    52981 tttttgaaac caagaccctg ggaggagtaa caggcaccag gctgaatcca atggctgtaa
    53041 gctttgttga aagctatatg gcaaaatacg gtaaaggttt gcggggtatg agaaattggg
    53101 ccaagcctta ttttgatatg atcgataatg tgctgacgca acatgggctg ccaaaggaac
    53161 taaaatatct cgcagtaatt gagtcaggcc ttcgatataa tgccatatca tgggcgggag
    53221 cagtgggtcc gtgggccttt atgccggctg ccggaaggca atacggtctg attgttacga
    53281 atggctacga tgaaagactg gattattata aaagtacgca tgcagcagcc cggatgctca
    53341 ctgaccttta cctgaaatac caggattggc ttctggtaat tgcagcctac aactgtggtc
    53401 cgggtaacgt agataaagca atcaggcgta gcgggagcaa agatttctgg aaattgcaat
    53461 atcacttgcc cacagaatca atgaaccatg taaaaaaatt catttctacg cactatgttt
    53521 ttgaaggaga gggcggaatt acaacagtaa caaaggaaga aacaaaagaa ttgctgttaa
    53581 attatccctc tacgctgaaa gaggaggaat tcaaaaccag tacagcttat aagattacag
    53641 gtcggttcaa ctcatctgta attgttaaat ataccgggat agaccaactg actttcaacc
    53701 ggtataatcc tggtttcaat gatactattg ccctgaacgg cacttatgaa cttcggttac
    53761 caaccgaaaa gatgaacatt tttattgcta aaagatacca gatactcgaa gaaagtatgc
    53821 agttactgct gaacccggca ggcagtaatt cccggtaaca aattataatc gctatatttt
    53881 ctccagaaca tacttaatag cttctgcttt caatacacac tcttcccatt ccaattcggg
    53941 gtcactgtag taagtaatac cggaacctgc ttcaaatgaa agataaccgg atgatgcatt
    54001 atacatgata cttcgaatca ccacattaaa atcaaaatct ccatccggag aaatataacc
    54061 gatagcaccg gaaaaaattc ctcttttcgt tctttcatat tgctctatga gctgcattac
    54121 tcttttctta ggtgcaccgg tcattgaccc catcggaaat gtggccctga tgatctctga
    54181 aaagggcacc tcctctttta atataccgct caccgtagat atcatctggt gtacctgcgg
    54241 aaaggaataa attccataaa gctcatccac tttcacagtt ccttctttac aaatttttgc
    54301 aaggtcattt ctcaccaggt ccactaccat tacattctca gaacggtctt tggcactgtt
    54361 tagtaatccc actttgcttt tttgatcctc cgctgcacta ttcaatattc ttttagcagt
    54421 tcctttaatt ggttgcgaaa gaatctgagg cccttttctt ttcaaaaaac gttccggact
    54481 ggcgcatatc agccatttgt catttagttt atacaaagca gagaagggat tgggagatac
    54541 cttactcaat tttttatata ctgaaaatgg atctatgact gcgttctccg caaaaaattc
    54601 ctggcaaaaa ttaatttcat agcaatcacc tcttaatata tgctgcttta attttttaat
    54661 aatagaaata tattctttct gtgaaatcag gctttgtata gtaaaagaat acggtccaac
    54721 ttcatcggta gctgcacaaa cttcagttat gcccttatat accagatggg gattgtccga
    54781 ctcaatcacc aattcagttg ccgaaagcca tatgaccact tcgggttcaa agaaaaataa
    54841 gtctggaaat tgtattttat caggatttga tgaggaaagt tcctcaattt catttttcag
    54901 atcatacccc aggtggccga ataaccagct attgcctgca tcaaggaaag tttgcagttg
    54961 ctccagggca tcaccagtat tggcggtaag ctgtcttttt acacctgctg ccaacaggca
    55021 ctccaccgtg tgaggttcat gctggtattg atgattatcc agaaaacaaa aagtgttgaa
    55081 ccgttttgcc cagttcaaca ctttttgttt catctgccca aaatcactta cagtaaagtt
    55141 gcaattagtt ggggttgtgt tattcaatgt atgtgaccaa ataagtacta ttaataatca
    55201 tcgtcatcat catcataatc tttgtcgtca aataagtcct taaactcatc atcttcaaat
    55261 ttaaagtcgt cctcttcggc tgctttcttt ccggcaggag ctgccccacc tgcttttttc
    55321 ccttttgatt ttggaatatc aaattcatca aagtccgggt cccactcttc ttcctcttcc
    55381 ggcttttccc aatcatcggg ttcatcttcg gcatcttctt catcgtcttc atcaccactt
    55441 ttcttggaag cagcagcctt acctcccttt ttagaaggct ttacctcttc atcatcatca
    55501 atgtcatcat catcatcctc ttcctcttct tttttagaag gtttagggga agacttctta
    55561 gccgcctctt tttcagatgc tgcatttgct ttgccggttg tctttttttc ctttactggt
    55621 tttgtggcca tagtttaaca ggattaatgc tgtaaaattt cagcgaagtt tttaatccgc
    55681 caaattttta aattgttttt tttctgtttc acaaactccc ctgattttca caggtggcaa
    55741 gcaaaaatga aaaatttatt ttacctggat tatctgatcc cgtccgggtc cgttggatat
    55801 gaaatgaata tctacaccaa ggtatttatt gataaagtta acataggttt tcatcgtatc
    55861 aggcaattcc gaatacgttt tcgctgcact ggtcgttgta ttccatcctg taaaggcttt
    55921 atatacaggt attggctttt gtctctccat ctggaaaggc acttcctggc tttcttttcc
    55981 gttcagcata taagcggtac aaacttccag ttccttaaaa gcatctaaca catcagcctt
    56041 tgtcatgacg atctttgtga ccccatttat catacaggca aaattaaggg cgacgaggtc
    56101 tatccaacca caacgacgtg gcctgccggt tgttgcacca aactcatttc cgattttcct
    56161 aagctcttcc cctgtttcat tctccagttc tgttgggaaa gggcctccgc ctacacgggt
    56221 acaataagct ttggttacac cgatcacatc tcttatttgc tgaggtgcta tgcccagccc
    56281 gttacaaaca ccggccgaaa ttgtactgga agacgtaacg taagggaaag ttccaaaatc
    56341 aatgtccagc atacttccct gtgctccttc tgcaagaatt cttttacctg cctttatctg
    56401 gttattgata aaatattcac cgtttacaat attcagctgc ttcaggaatt caactgtttc
    56461 aaagaattct tcttcccatg cagtaatatc ctcctggaaa ttataattat ccagcaatcg
    56521 ctggtgcttt aagcgcagtt tgatatactg tgtagtaaag tttttatcca gtaaatcgcc
    56581 tacccgtaag ccatttcgtc ctgtcttatc catataggtg ggtcctattc cttttagggt
    56641 ggaaccaatt ttctgatttc cctttgaaag ctcggcagct ttatcgaggg ccctgtgtgt
    56701 tggaaggatg agatgtgtcc gctctgcaat gaaaagattt tttttcagat caatgccaaa
    56761 agaggccacc gtatcacatt ctctttttaa cgttaccggg tcaagtacca caccattgcc
    56821 gataagatta atgataccct catgaaacac accggaagga atctggtgca acacaacttt
    56881 tttatcatta acatataaag tatgccctgc attggggccg ccctgaaaac gggccacgag
    56941 gtcataatct ttagcaaaaa aatcaacaat ctttcctttt ccttcatcgc cccactggag
    57001 gccaagtatt acatctacca tgattcagtt gtcagttatt tttctcttgt gcctctttga
    57061 aattggcggg ccagcctgcc gtagcttcca acttcattag aacttcgttg gattaattag
    57121 caaaggcagg gcaaaaatac gggttcacaa aaaagccacc gaattaaatc cggtggcatt
    57181 tcatttattt tattcccaaa aaaattgact tattaacgat ggaaaagcag cctaagctcc
    57241 tatcgtatca gcattactgt tcctttcttc gttattacac gattatcttt ggtaagacct
    57301 tcaatcatcc acacatatac accactgggc tgttcctgac cacccaaacg gccatcccac
    57361 ccatcattaa gttttgtagt tgaaaagacc atctggcccc agcggttgaa gaccctgaaa
    57421 taattatagc ttttcatacc cacagggaag ggtgtgaact tatcgttctt gccatcatta
    57481 ttaggcgaaa atcctgttgg cacataaata tccgggcctt tatacaccct cacttttaca
    57541 tagccttctc cccggcaacc tgcaatcgtg gaagttataa cctggtattg aatatcctct
    57601 ccgattgctc caattgttac tataggattg ggaatgttca gattacctgt tccaatggtt
    57661 gaattaggat tgcttaatcc gatggtggga ttccaggtat aattgatgac atcactatct
    57721 gatggaacaa ccaatatctg gaactggtca ccagggtaac cgatggtgtc gtatggatag
    57781 gtctttacct ttatcggggg cgtcacatct attcgcatat tatctgtaac cagcgaagca
    57841 catccatttg catcagacaa aatcgacaaa gtataagtaa tatcctttgt aggtgtggaa
    57901 acgggatttg cactattggc atcatccagg taagtggaag gcgaccatga atattgtgta
    57961 ccaccggaag cactcaactg gtaggtttga ccatagcaga tataaccatc aacacctgca
    58021 ttgggaaccg gggctgcatt tacatttacc gttactgtat catttgcgct gcaacgaccc
    58081 agcgttgccg taacaatata ctgggtagta acagtgggat ttgctaccgg gttatagatc
    58141 gaagcattgt ccaaccctgt agccggcgtc caggcatatt gagtggcatt ggaattcaat
    58201 tgcagttgta cggatttgct ttcacaaata accggatccg cttgctgtgc caatgtaaga
    58261 ttatcggtaa ggccaactgt cgtattgaaa gtggtgctgc aacctaaatt atcctttaca
    58321 gtgaccgtgt aattgcccgg tgccacatta aactggttag aggactggaa attaatgcca
    58381 tcgatggagt aggaataact gctgttgcca ccgttggcgg ttactacaat tgttccatca
    58441 tttccaccat cacaacttgc attcgtagca acagcattgg cggctaatac cgcaggctct
    58501 gttattgtag tattctgggt agaaatacag ccattcgcat ccctgatagt tatcgtatag
    58561 ttaccggcag gaacattaaa catattgctg ccctgccagg tagtaccatt cagtgaatac
    58621 tggtaaggag aaacacctcc gttagctgct actaatatgg taccattgct ttgtgcatta
    58681 caaatagcgg cagacgctga tacagaagtt gccaatacag taggttctgc tactataatc
    58741 ggtaatgttc cctggcaacc accaccttcc ctgaaataaa cggtatatgc accggccgcc
    58801 aagccagtga agacattgct agtttgccag gtggtaccat ctaaagaata ctcaaaagga
    58861 gctgctccta ccattggttg aactacagtg atcgtgccgg ttgcacttcc attacataag
    58921 gcatctgttt tactggcagt agttgaaata ggcggacctg caactactgt aactggaatt
    58981 aatggggtaa cacatccaaa agcatctgtt acaaagattg tatgagcacc aggcggaaca
    59041 ttggtaaatg tataaggcgc cgggccggta acaggagtac ctccatccat tgtataagta
    59101 aatggaccag tgccggtggt tggagtcact gtaatagtac catcagcaga accattacaa
    59161 gaagtggcag ccgatgcagc accagcctgc atttgtgtaa gattcaccgt tacagtatca
    59221 taagaaataa gctggttttg tgtgccgtca caagatgaat aggtagtcct gactacaaaa
    59281 tttgtcgagc ttccgttgac acaaacattt ggaaaactta aatcaagcaa tcctgttgtt
    59341 gttgtgcttg tatcggctgt agctaccaac gttccgccca tagtcagtaa ctcagaggta
    59401 acatatcttg atgtacctgc tgatggaata aacttataac cggtattact ctcattccat
    59461 acggtattgt tctttccggg tgcagcaata gcctggtttt gattccagtt ttgtataccc
    59521 aggatagctc ttcctgcatt agtggatgat gcacatgatt tttgctgaaa aaatatttca
    59581 ataattccgg ttgattcatg cattaccatt tgaaaagtat tggcaggaac cgggcaggaa
    59641 gacgctgaaa ccccaaacac accgatatga taataactaa ccacaaattt ccggcatgga
    59701 gcagtaccga tcacttccca ttgtattttc ctggtagaag gacttgctac tgtccgggga
    59761 tctaaatctg aataagcacc catgatggat gcccggggat agtaagcagg aaatgaagtg
    59821 caaatattcc ctccggcata tggaatagtt tcagttatag gccaggcatt actacaagca
    59881 gcattagccg gatcaaaagt aactatgcca ttagagccta ctgctgcact tgtataggat
    59941 tgcccgtaaa aacagaaggt aaaaggtaaa tttataagac tgctgtactg gtcgtcatta
    60001 tacaacgcaa tatctgttgc ccctgttggc gtgctatagg gatatggagc ataaggtgta
    60061 gtaaccaggg tgtaatctga agatgacttg atatgcggga tctgaaaggt taatgcatta
    60121 caaatttgtg agcaatccct attgattaca gccccattta tattggtggg aatacaattt
    60181 tgtgcagcaa tttcttttga aaaaagaaaa acaaagccca gtacggcaaa aacttttaac
    60241 agtctcagtc tcataaaaac agatgcagtt ggtatggtaa aatcagttcc tgaacggcct
    60301 tcaagataag taaaaaatgg gtctatgcgg aattttgcgg cttgattgtg aacaaaaaga
    60361 gaataggaaa aatacggtca tggtttaatt gatgagtatc aataccctta tgaggggata
    60421 tcttccatat catatctttc cactgattca atacccgaaa ggctgagtaa actttgcacc
    60481 aggttgtcga gttcttcttt atcatgtaca tataacctga tattaccttt gaacaatcct
    60541 tcgttagcct caatcgtcag tgccgcaata tttattttta actcaccgga aataagattg
    60601 gtaatcttat ttaccactcc cacatcatcc ataccaacga tcttcacccc tgtcaggaat
    60661 gatatttcct tattcttagc ccatttcgtt ttaactaccc ggtgcccata attagccagt
    60721 agtttggctg catttggaca gttggttcgg tgaatggtaa gtcctttacc ggttgttaca
    60781 aatccaaata catcatcacc cggaataggc ttacaacaat tagccaggtt gtatactatc
    60841 ttatcactgc tttctccaaa tattaccagc tccgcatcct ttttaccagt aacatggtgg
    60901 ggtaaaaaat catgtttctc attatgcaat tttaccggcc ttggagcctc tatcctgtcg
    60961 ccagcaatct tgaaatcctt tatatctttt aaatcaatat tctttaccgc cacctggtaa
    61021 aacaggtcca ggtttgaacc cagtttgtac caatgcacca gctcatcaat attatgctgc
    61081 gacaaagaag ctcccatgcc ttcgagtttt cgttgcacag catatttacc ctcatcggcc
    61141 acttttcttt tttcttcttt cagtgcatcc ctgattcttc cttttgcctt ggaagtaaca
    61201 acgaacccca gccagtcttc cgatgctttt tgtttgttac tggtgatgat ctccacctgg
    61261 tctccgctat ggagcttttg acttattgga accagtttat gattaacttt tgcgccaatt
    61321 gttttgacac cgatcgcact gtgaatggca aatgcaaaat cgagtgcagt ggacccaacc
    61381 ggcaacattt ttacatcgcc tttgggtgta tacacatata tttcttctgc aaggaaactg
    61441 gttttaaaat cctgcagaaa atctatactg tccgtttcct ggttggtgat cacttcccgt
    61501 atttgctgaa accatttatc aaaacggctt tcatcagtgg cgccttcttt gtatttccag
    61561 tgagcggcta atcccttctc cgcaatttca ttcatccttt ttgtacggat ctgcacttcc
    61621 acccacttgc cctgcgggcc catcacggtg gtatgcaatg cttcatagcc atttgcttta
    61681 ggattactca accagtctct tagcctttcg ggtgatggat tatattcatc tgtgatcatg
    61741 gaatatactt tccagcaatc ttctttctct ctttccggcg gtgaatcaag gatggcacgt
    61801 atagcaaaaa gatcatacac ttcttcaaac cccactcctt tctttttcat tttgttccag
    61861 atggagtgga tgcttttagg acggccatgt atttcggctg taagatttgc tttttctaat
    61921 ttatccctga ttggtttgat gaattcattg atgtacttgg ttctttcctt ttttgtttcg
    61981 gccagtttac gggcaatttc tttataggtg tccggttcca tgtatttcat ggccaggtct
    62041 tccatctctg ttttaatagt gtataagccc atgcggtgcg ccagcggagc atatacatat
    62101 accgtttcag aagatatctt gagttgtttc tcccgcttca tactgtccag tgtccgcata
    62161 ttatgcagcc gatcagacag tttgatcaaa attacacggg ggtcatcggt tagtgtcagt
    62221 aatatttttt taaaattctc tgcctgttgt gaggcattca catcaatgac gttggcaatt
    62281 ttggtaaggc cgtcaacaat ccgggtgatc tcctcgccaa attctctttt aatatcatcg
    62341 agtgaaatgt cggtatcttc taccgtatca tggagcaacg cacagatggt ggagcgaata
    62401 cctaacccga tctcttcggc acaaattttt gctacagcta tcgggtgaag aatataaggc
    62461 tcgccgcttt tacgacgcat ggttttatgc gcttctgccg acatttcaaa agcagttctg
    62521 accagggctt tatcacctgg ctttaatttg gattttatca ccttcagcag ggatctgtac
    62581 tccctcagga tgagtttttt ctcttcctct tcgttcaggt tatacttcgg tatctgtgca
    62641 acggtttcca tggtctacga atttagtgaa ataagccaag aggtcaaaag agcaataggg
    62701 ataaagagac gtatcacggg aaataccagt tcccaaatga aaaactgata acaataatcc
    62761 tgaaaaaaag gttaacaacc catccttttt ctcctttaca cttttcatcg ctaatccccc
    62821 tgcttaagaa attcatactt cgtgattatc gggatggtaa atatggaggc aagattttta
    62881 catagaccaa cctgacagaa caagacccaa aaaggagtaa atactgcctt ctcctcaaca
    62941 aatagtttcg tgcaacaagt ttagcgcaat acgttacacc agttgtccag ggaatcaaat
    63001 ttttaaggga agcccagtgc cttgtcgaga atattcgggt gatatgagta acaaagtcaa
    63061 acgataaaat gtagtcaata tttgaagcat tttgcgacta tattgcggtt ttctgcaagc
    63121 ccaaatattt tatctcttca tcaatctatc cggtaagcta aaatgtccta catttgcagc
    63181 cccaaaaccc ggatgtggtg aaattggtag acacgtcaga cttaggatct gatgccgtaa
    63241 ggtatggggg ttcgagtccc tccatccgga caatcaccag ttcaaggttt gaggtttgaa
    63301 gttcgatgtt aaactcaaac ttcaaacttc gaactttaaa caaataatat ggctacagta
    63361 accaaagaaa acatcggcct gctgcatgaa aagttaaccg tgaagctgga aaagaccgat
    63421 tacctgcctt cttttgaaaa ggcgttgaaa gagtacagca aaaaagcaaa tatccccgga
    63481 ttcagaaaag gaatggttcc ttcaggttta attaaaaaaa tgtacgggcc ctctttattt
    63541 actgatgaaa tattgaagtc tgttgaccgt gaactgatcg gctacctgca aaatgacaaa
    63601 cttgatattt ttgcccagcc cctgccattg gaatccgatt ttcgtcaact ggatgtaaac
    63661 aacccggcag attatacttt ccattttgaa atcgggatga aacccgaatt tcaattacct
    63721 gatttagcaa aagcaaaaac aacccgctac gttgttactg ttacagatga aatggtggac
    63781 aatgagatca cccgtttgca gaaccgctac ggcaatatga aagatgaaga gacagtaatc
    63841 tctgaagaaa atgtgctgaa tgtaaatttt acagaagcag atgcggatgg taatgaagtg
    63901 gaaggtggta ttaaaaaaga taattctatc ctggtaaaat attttgcaga aggattccgc
    63961 aaaaactgga tgggtaaaaa aacaggtgac acagcaactg cacaattaag aacagctttt
    64021 gacgaaaaag aaagggagtg gatcattggc gatcttggat tgaataaaga agatgcaggc
    64081 actgctgaca aatacttcaa gattgaaatc acaaaagttg gtttaatgga gaagagagaa
    64141 ctgaatgaag aatttttcaa ccagctttac cctaaccagg aagtaaaaac agaggccgat
    64201 ttcagaaata aaatcaaaga agaaatacag gcatattgga acggacaggc cactaaccag
    64261 atccatgacc aggtgtttca tcaattggtg gaccacacaa aaattgaatt cccggaagga
    64321 tttttaaaaa aatgggtgaa gactcaagga gaaaacccca agaccgatga agaggttgaa
    64381 aatgagtttc cgaaattcct cagtcaatta aagtggacct tgatctctga caaaatcgtt
    64441 caggaaaacg ctattgaggc aactcctgaa gaaatcagga attttgcaaa acaacagctc
    64501 ttcagttata tgggaggagc caaccttagt gatgaccagc cctgggttaa tgattatgtg
    64561 gaaaagatga tgaaagacag gaaatatgtg gaagatgctt ataaccgcat ccagacacaa
    64621 aaaatatttg agtgggctgc tacaaagtta aaccccactg acaaagacat cacagcagat
    64681 gagtttacta agatggtgga aacacatcag catcaccacc attaagtcat taagaccaca
    64741 agtcagaaag tccggaagaa ttattcaaaa aacagaatag gcttccggat ttctcgtctt
    64801 tgctgacttt cagacttctc ctgtaaagcc atcttgtcat tgattttgca attacgccat
    64861 ttggacggat atttgaggag tttaaatgtc tactgttcaa ggagaaagaa tcagctgatc
    64921 aggaaattta aacactaaat cttaaaactt tcactcacat gacctttaat tcggaatttg
    64981 aaaaatatgc ggtgaaacac cgtggtattt ccagcaatac cctcaatagc tatgcctctc
    65041 atctggtaac ggctatgact cctaatatta ttgaagaacg ttcccttaat gtggctgtta
    65101 tggatgtata cagccggctt atgatggacc gtatcatttt tttaggttac cctatcaatg
    65161 acgaaatagc taatatcgta actgctcaat tgttattcct tgactcttct gacagatcga
    65221 gagatatcaa tatgtacatt aacagccctg gcggcagcgt gtactctggc ttgggagtat
    65281 atgataccat gcaatatgta agccctgata ttgccaccat ttgtattggt gttgccgcat
    65341 cgatggcctg tgtattgtta ggcgccggca caaagggtaa aagggccgct ttgaaacatt
    65401 cccgtattat gatgcaccag ccaagcggtg ccattggcgg acaggccagt gatattgaaa
    65461 tcactgtgaa tgaaattcgc aaactgaaaa gagaattata cgaagtagta aatgtccata
    65521 caggtaaatc aattgaacaa attgaaaaag attttgaccg ggacaactgg atgactgcag
    65581 ctgaagccaa agaatatgga ttggtggacg aagtgctgct gataaatcca agaaagcaga
    65641 agaaagatta attattgatg tataaatctt aaactattaa actctttaca tgaatcgcaa
    65701 acaattttta tacgctgact ggagaatgga tgaagaagaa gagaaagagg aacaacccaa
    65761 aagtgatctt ggtatgttca gtaagaaatt agaaagatat ttttttgaaa aaagggctgt
    65821 ttatctatgg ggtgttgtgg atgacaaaag tgccaaagat gtagtaagca aactgttgtt
    65881 gctggatgcc gacaaaccag gggaagaaat aaaattttat atcaacagtc ccggtggtgt
    65941 agttaccagt ggcatggtga tgtatgatac gatgcaaatg atccagagcc cagtaagtac
    66001 aatttgtatg ggcctggccg cttctatggg cagtatttta ttaagtggtg gcaaaaaagg
    66061 aagccgttat attttcccgc atggtgaggt aatgattcat cagccatctt tgggtggata
    66121 cattcagggg gttagtaccg atttggaaat tcaagcggag caaacccgga gagtaaaaga
    66181 gattggagct ggtattttag ccaggaattg taataaaaca gtggaacaga tcatgaaaga
    66241 ctttgacagg gattattgga tgaatgcaaa tcaggctatt gaatatggca ttgccgataa
    66301 gctggttgaa aagctataat cttctcttta tcaaatattt aggttatata aataaataaa
    66361 accgccctta tcagggtggt tttataattt tgtagattga gaattgattc tcagactcct
    66421 tcaggttaag ttaaggttaa taaattatgg caaaaaaccc attacattgt tcattctgtg
    66481 gcaggagccg cgatgaggta aagatcctta ttgccggtca ggaaggacat atctgcgaaa
    66541 actgcgtaga acatgcccgg gagattattg accaggaact tgcagtaaaa gatgataaaa
    66601 gcacttcatc tttcaaactc actgtaaaga aaccaattga gataaaaaaa ttccttgacg
    66661 aatatgctat aggccaggac gaggccaaaa aagtattagc tgttgctgtt tacaatcact
    66721 acaaacgttt acaacaaaaa acagctgatc ttaattccgg gaatgaagtt gaaatagaga
    66781 aaagtaatat tgtaatggtg ggtgaaaccg gaaccggtaa aacattactg gcaaaaagta
    66841 ttgcccgcat gctgaatgtt ccttttgcca tcgtagatgc cactgtattt acagaagccg
    66901 gatacgtcgg cgaagatgtt gaaagtatcc tgacaagact tttacaggta tgcaattatg
    66961 atgtatccgc tgctgaacgg ggtattgtct acattgatga aatagataaa attgcccgta
    67021 aaggcgataa cccttccatt acccgggatg taagtggtga aggtgttcag cagggaatgc
    67081 tgaaattact ggagggaaca gatgtactcg ttccgccaca gggtggcagg aagcatcctg
    67141 agcaaaagct tattaagatc aatacacaaa atattctttt catctgcggt ggtgctttcg
    67201 acggtattga taagattata gcccgcagga tacagaccaa cacgataggt tttaatgttg
    67261 ataaagaatt gcaggataat atgaagaaga acctgctgag ttatgtaaat gcaacagacc
    67321 tgaagagctt tggtttaatc cctgaacttt taggacgttt acccgtaata acacatcttg
    67381 aaccgctgga tgctacgacc ctgagagcta ttcttacaga gcctaagaat gctcttataa
    67441 agcaatacac aaaattgttt gaactggagg gcatccaatt gacaatagaa gaagaggtac
    67501 tgaatttcat ggtagaaaaa gccatggagt ttaaattggg cgccaggggc ctcagaagta
    67561 tatgtgaaag catattaact gatgcgatgt atgaattacc ctcctctaag gaaacacatt
    67621 ttaaccttga cctggaatat gcgaaaagaa actttgataa gagtaagcta agcttgctga
    67681 aagtggctta atacaaaata ttttcaaaaa aatgctgagt caaatactca gcattttttt
    67741 tacttcttaa cctgtagatt aattggtacc agttaatgtc aaagtaaatc cagtagtaac
    67801 tgtaagcgaa gcgccgttat tcactgttat ttttttaaca gaaacatttg agttaataac
    67861 tggaagaaaa ggggagccgc tggaaattat tacttcgcta ttagcatctg gcaggatgcc
    67921 acaactccag ttcaacggat tttcccaggc atcgctgaac gagccaaccc agttgatacc
    67981 tgctgccgaa caaacacttt ttacccatgc gtatccatca gtataagcca cagagggtat
    68041 atcatttcct attacaaggg atatattatt agcgacacca gcagaaattc cttctgcatt
    68101 tacggttgtc catgtacctg ctgtaaatct ctttattgtc aacctgttgc cattggctgc
    68161 atcctggtat gccacatacg gagttccatc tgcagtaaaa ctaagtgata gattggtagc
    68221 agcgccggtt gaaaaacccc ctgcaggacc aacatttacc catgctgcac catcataggt
    68281 ttttacagtc accttattac tattacttgc atcagcataa ccgatgtaag gattcccact
    68341 acccggagca atgccaagta caatgaatgt tgcaacacct gctgatactg gctgtgttcc
    68401 caaattcacc caactgctgc cattatatgt tttaacgatg gctttatttc ctatagatac
    68461 atctgcatca ttataggcta caaaaggtgt tcctccgggt gaaagtgcaa gtgaaacaaa
    68521 ggaagccgac ccgactgaga ttggctgtgc tcctgtattt acccaactac tgccattaaa
    68581 tgatttaact atgactctgt ttgaaaaact aacatcggca tctgcatatg ctaaatatgg
    68641 agttccagtt gtattgattg ccaaggaggt atagcttgca ctacctgtag aaaatccctc
    68701 tgttccaatt gttgtccatg cattatctgg tgcaagaaat ttgacggtcg cttttccgga
    68761 attggcccca tcctggtatg caacataggg ttttccgtcg gccgcattaa acgaaagtga
    68821 tataaaatta gcagtgccgg aagaaaagtc ggcattacct gcaagattcc aggcgccgga
    68881 aacataattt tttacggtag ccttattgct actaccctta aaaccaacat acatatatcc
    68941 atctgtccca aaaacagcag aagtgtatcc tgccggtcct tcagaaagcc cggtagcacc
    69001 aaccgttgtc cagtcagctt caagtttttt cactgttgct cttgaattat ttgttgcatc
    69061 ctggtatact acataaggta ctccatcatt tgcaatggcc atcgttgtat actgtacccc
    69121 acccaaagaa aagccggcag tcccggcatt tacccagcta accccattga attttttaac
    69181 cgtggcttta tttccgtttg caacatcttt gtacgaaaca taaggagtaa agtcttgtgg
    69241 gtcaattgcc attgaaacat attcagcagc gcctgttgaa aaacctatta ctgcccctac
    69301 atttacccaa tttgctccgt taaacaacat cacatttgct ttaccgctac gtgttacatc
    69361 ctggtaagct acataaggaa ttccgtccga agtaatggca atggaaggat atttgataat
    69421 agcattattg gagaaacctc ttgctccaac caatacccag tttgcgccat catatttcat
    69481 cacagtggct gcatattgcg tagtcgcctg gtcatccctg aataaaatat aaggagtccc
    69541 atcattctga tcaatggcca atgaaagtgc catcgcctgt gaagagcctg tgaagccagc
    69601 agctccaaca gtaacccagt tggcgccatc atactttttt actgtgcctg ctactgccaa
    69661 tgtataatct ccataagcaa caaacggagt gttatccgga gcgatagcga tggcaatatt
    69721 cggataataa tcaggaaacc ctgaaaatgc aatctgttgc gcatttactg aaccaacaac
    69781 agaccatgta cccgatgagt atttcatcac tgttgcatcc agcctgaatg gatcgccgcc
    69841 attttgtgtt tggtaaacca caaaaggagt accgtcaggg gctattgcaa tctgggcata
    69901 ttcaatctgg gtggatgaaa agccggcaga tcctacaatt tgccaggatc ctgaagtata
    69961 cctggctaca gttgccttgt taccattacc cccatccctg tataccaaat aaggtatacc
    70021 cgcagcggta tctatagcta tcgaggtaaa agtagtagga ttgaaataag aaggttgatt
    70081 gaaatcatcc ggatcaaggg gtatccatga ttgtgcaaac agggtataat gcatcaacat
    70141 tatcactaat gttgtaatat attttttatt aattctcata aagtatacca taggaggttt
    70201 aggcaacatc attaaaattc aggaattgaa ttcaaaagga tcttcgtcta ggaatttgga
    70261 gaaattatac ggattcacca gaagcgttga aaagatttgc aatatactaa aaatatcata
    70321 tttcacaaac catatggcct cctttatcta tacaaaaatg acattcattt tcctcctttt
    70381 ttaatattgt acgcagtttt ttcctttact tttaataaaa aaagaacctt atgcaggagt
    70441 taatagagaa attaaaagca gaagcaggcc ttacggatga gcaggcaaaa aaagccatta
    70501 ccactatcaa aaattttgtg gtagaaaaat ttcccatgct ggaaggcgct gttaataatg
    70561 tattcggggg agattaattc gtcctgatcc cgaagttctt attccaataa aaatatatga
    70621 ttacttaatg gtggcgcatt ttttcccccg gggatgatta gctgcatcat aaggtgtcat
    70681 cgcacggctg caggattgaa atattatact tccgataagc aggaggaaaa ggactctgtt
    70741 tctcatagtt acaaattgag gtatgaaagt aatacctgtt ttgagaaaac tggcaggaaa
    70801 tccctgctga aatacacttt ttctaaagcg gaaaactacg ggttaaaagc tacttcttta
    70861 ctcttttcaa tttaatgcaa tagttttttt taaaactact tctataataa aaaacatccc
    70921 ggcttttttg ccgggatgca gaattaatgg gtaccttctt tatttggttt tccgtccttt
    70981 actttttaac cacaaccacc ctggttgccg cagttaaggc atttgtagca ggcgccgctg
    71041 cgcatcatga tattaccaca cacattacag gctggtgcgt cactttgcat attcttagcc
    71101 accgcattca tagcatctat acttgattcc gtcttaaccg gttgggaagc ccgttgattt
    71161 cttaccggtt gcggagctac agttccggac gaaggagtga tacgaacact tgacaactcc
    71221 ggatctttta catattccaa cggattagaa gggacttcat cccaatcatc accaccggta
    71281 ttcattactt ccggtttatc cagtacatga accaggtctg tacggcccag gtattcatag
    71341 cctaatacac ggaagataaa atcaacaata gaagtagttg ttttaatatt cgggtgctct
    71401 acaaagcctg atgggtcaaa ccgggtgaaa gcaaatttgt caacaaactc ctccagtggc
    71461 accccatatt gcaaaccgat agaaacagaa atcgcaaaac agttcatcat actgcgcatc
    71521 gtagcacctt ctttcgccat gtccacaaag atctcaccta atgttccgtc tgcgtattcc
    71581 ccggtacgga ggaaaacagc ctgaccgctg atctttgctt tttgtgtaaa cccacggcgc
    71641 ttggctggta atgtgcggcg ttcaacaatg ctggccagct tccttttcag cttggtatcc
    71701 ggagaagcct gtaccctttt ttgtacctct tccagcaatt cttcaacagt cagtttacct
    71761 aaatcaacaa tattggattc aacctgctgt tcaataacca ctgctttttc ttcttcggca
    71821 acaatatctg cttttttctt cttatcagat ttgttgctga gtggttgtga taatttagaa
    71881 ccatcacgat aaagagcatt tgccttcaga cccaatttcc agctcaggta ataagcatct
    71941 gcaatctctt ctactttagc ttcatggggc aggttgatag ttttggagat ggcgccactt
    72001 ataaacggct gggtggctcc catcatgcgg atatggccat gagcatggat atagcgctga
    72061 cctttctttc cacatttgtt ggcacagtca aaaacgggca ggtgttcttc tttcagtccc
    72121 ggtgcacctt ccagcatcat ggttccgcat acataatcgt tagcggcttc aatctgctca
    72181 tcagtgaatc cgagtgcttc caggaggctc cattcaaaat tattgtattg ttcaggggta
    72241 aagccaagtc gttccaggca ttcttcaccc aaagtatatt tattgaaaat aaacccaatc
    72301 tcaaaagcgg tgagtgcggc tgcatcaagc cgtttgatct catcagcaat aaatcctttt
    72361 tcactcaatg tctggtgatt gataaaagga gctcctgcaa aggaggcaga acctgttgcg
    72421 tatttaataa tagcctcgga ttctttttca aagtaaccta aatttttaag agcagcaggt
    72481 actgactggt tgatgatttt gaaataaccg ccaccggata attttttgaa tttcaccaaa
    72541 gcaaaatctg gctctacgcc ggttgtatca caatccatca ccagcccaat ggttcctgtt
    72601 ggtgcgatta cagtggcctg agcattcctg taaccatatt tttcacccag ttccactgca
    72661 tcatcccatg ctttgcatgc agcttttaac agataatcgg gacaatattt aacggtaata
    72721 ccctgtggtt tcaggctcag gttttcatac tcatctgcat cataggcagc aaggcggtga
    72781 ttccgcatta cccgcatcat gtgggctttg ttctcctcgt atctcggaaa agcaccaagc
    72841 acctgtgcca tctctgcgga tgttttgtaa gacgtccctg tcatgatagc agtgatagct
    72901 ccggcaatgg cccttgcttc ttcgctgtca taagggatgc cgctaaccat cagcatagta
    72961 ccaaggttgg catagcctaa tcccagagtt ctatactcat aacttagctg tgctacttct
    73021 ttagacggga actgggccat caatacggat atctcaagca ctactgtcca caaacggcag
    73081 ttgtattcgt aaccctccac atcaaactgg ttcgtttctg catcaaagaa cttcatcagg
    73141 tttgcagaag caaggttaca ggccgtatta tcaaggaaca tatattctga gcaggggtta
    73201 gaagccctga tctccccgcc ttcgggacat gtatgccatt cgttaatagt ggtattatat
    73261 tgtgttccgg gatctgcaca actccatgca gcataggcaa tctggttcca tagctccctt
    73321 gcaggaattt ttttcatcac tctgccatcc attctgccgg taagttccca atcttcatta
    73381 gcatccagct tttcaaagaa ggaattgggg atacgaatgg aattattaga attttgtccg
    73441 cttactgtcc ggtatgcttc atcctcatat ccggaaggat aaccggcagc gatcaatgct
    73501 cccacttttt tctcttcctc cactttccag ttgataaact ccacaatttc cgggtggtcc
    73561 agatccaggc aaaccatttt agctgcacga cgggtagtac ctccagattt gatcgcacct
    73621 gctgcacggt cgccgatttt caaaaagctc atcaaaccgg aagagcttcc gccaccactt
    73681 aatttttcac cagagccacg aaggttggaa tagtttgttc caacacctga gccatactta
    73741 aagatccttg cttcacgaac ccaaaggtcc ataatacctc cttcattcac aaggtcatcg
    73801 tcaacactta aaataaagca ggcatgcggc tggggacgtt catatgcgtt ctgtgatttt
    73861 ttcagctgac catcatttgg atctacatag tagtgtccct gtggtttacc ggtgataccg
    73921 taactttcat ataagcctgt attaaaccac tgcgggctat tgggcacaca catctggttc
    73981 aatatggaat aaaccaattc atcatagaaa acctgtgcat ctttttcatc gccaaagtaa
    74041 ccatatcgtt caccccatac tctccagcag ttagccatac gatgtgccac ctgctttgca
    74101 gaagtttctc ttcccaggct gccatcgggt tgtggcacac cggctttccg aaaatatttt
    74161 tgggctaaaa tgtctgtggc aatctgactc cactgtttgg gtacttccac attattcatt
    74221 tcaaatacaa cttcacccga cggattacgg attacagagg tacgataatc atactcgaac
    74281 atatcataca cattcacatc ctcacgggtg aagcgacgac taaattgcaa accgcttttg
    74341 gcggatgcct gcttcttatt tgccataact agaaagaatt tactgggttt ttggtcctgg
    74401 ttacaaacag accactctgg cgagaggtct gttacgaaag tattttttat aacatgaagt
    74461 tcaaaccttc aaatattaac attgtgtgga taagcaaatc gtaaaatccc gaagtgctta
    74521 cctgtattgg tttagctaat ttcttcacat ctactaatca atttttggac aaattttttt
    74581 tggaaaattc aatctcaact attaaaaaat tattaacaac tcacccttgc cctgaaacgc
    74641 aaaacaggta aggatttttt gtaataaatc cgctgaaagg ctttaccctg ctgtatttca
    74701 taaaaatata aacctgaaag ccttgcctgt cgtataacgc agacttaagc aaatgataaa
    74761 aaacaattaa tctcattatt ttgcaaattt gcttatatga tcaagtctat acttactgcc
    74821 cttattatcg tatgcagccc gttctttatt aacctgcatg cacaggcaca atttaccgga
    74881 tggctggctt cctttaatac cataaaaacc ggtaaaaaca caagcttgca taccgacatt
    74941 caattcagaa gtacaaatca atggcagcaa acccaaacaa tattgcttcg acccggtatc
    75001 aattttcatc tgaataaaag aataattgca actgcaggtt atgcttttat caataataaa
    75061 agaataatgg gaaatgttgc cggctttgca cctgaacata gaatttggga acagttatta
    75121 ttcaatcata aactgaaaac tatttttata agtcatcgtt tcaggattga acagcggttt
    75181 ctttccaaag tcattattgt gaataatgaa ctggaaacta acggttctgt ctatgctaac
    75241 cgtttccggt attttaaccg gaatattttc ccctttaaaa aagaaaaagc tttctcaaca
    75301 ggaatgtttg ctgctttaca gaatgaaatc tttatcaata ttggaaacac agcgaatgtt
    75361 aacggggaaa tctttgacca gaaccgattg tacctggcaa taggttaccg cccccatcca
    75421 aaagctgata ttgaaatcgg ctatatgaac caatacgtaa atggaaaagg taacctgttt
    75481 accaataatc atattctgca gttggctggg tacctgaggc tatgacaaaa agaagttttt
    75541 acctgccgca aagccaacta tttttcgcat ttttgcaata accaaccggt accggatgaa
    75601 gtcaaccatt tataacacat tggccgataa gaaaaagcaa ggaaaaaaat cctttgctgt
    75661 actcatcgac cctgataagg tgaacacatc ggtgctggat gaattgatag aactgtccat
    75721 ttcagcaaaa gttgactatt tcctcgttgg tggcagcctg gttatctcaa atcacctgga
    75781 tgaatgtgtg cagcacatca aacaaaactg caatatcccg gtaatccttt ttcctggcag
    75841 cccatcgcaa ataagcaagt atgccgatgc attattatat ctctccttaa tttcaggccg
    75901 taatcctgaa ttattgattg gccagcatgt ggtatctgct ccgtttgtaa gacagagcgg
    75961 attggaaata atgccaaccg gctatatcgt tatagatggg ggtgccccca ctactgtttc
    76021 ctatataagc aatgctgctc ctgtaccggc tgacaaaaac gaaatagcta tgtgcacagc
    76081 tatggcgggt gaaatgctgg gaatgaaact gatctatatg gatgctggca gcggtgctaa
    76141 aagacctatc acggaaagca tgataaaagc agtggcagac catattgaag tcccattgat
    76201 tatcggtggc ggtatcatcc agcctgaaaa agcttacctg aactgcaagg ccggcgctga
    76261 tgttattgtt gtaggcaatg ccattgaaaa agatgcttca ctaataaaag agatgagtga
    76321 tgcagtgcat agtgtacccg taaaggctta acgcaattca tccactttga ctctcgttgg
    76381 ggtttctttt cctgcgataa tactttttga acttaaggta atcgctttta tcacttcagt
    76441 catattggtc aggtcaagtg tttccacatg gtcagtcagt ttgtggtaat gaggttccac
    76501 atccattttt gaggtagaaa tcgtatgtgc cggtactcct aatcttgcca gggtggcatt
    76561 atctgaacga taaaataatt gctgttgagg gtatgggtcc ggataaaaag caaatgccgt
    76621 tcctttcagg tctttggcta atatttcacc catattggtc ttctcataac cggtgatata
    76681 ggccgagttc tttccccatt tactttcggt acctatcatt tcaatattaa acatggccat
    76741 gactttatcc gcatctatct gacgggagaa atattgcgag ccaaagcctc ctacttcttc
    76801 cgcagtgaag gcagcaaata caattgtcct ctcattatta ttcacttgct taaaatagtt
    76861 agacagcatg atcaaagcag ttattcccgc tgcatcatcg tttgcaccat tataaatgga
    76921 atctccgttc accggtttac ccacaccaag atggtcataa tgacctgaaa atatcacata
    76981 ctcatccttt ttactcttcc ccggcaacac agccactata ttagcaagct tttgctcagt
    77041 tatctcgtga gtggattcta tgtcgaaacg ggcaggatca gtatcagaaa gcacaaatac
    77101 cacattggta taagctttaa acatggacga tttaagcctt gatatatttc cgaagctgtt
    77161 gctaaaactt ttatctacca ataccagcag atttttattg gaacggacca tttttcttgc
    77221 ttctatctgc aaattagtcc cggctttaat cgacactttt tcgtaacctg aactttggtt
    77281 gatctttaag tgggattgag atgtaattac aataatattt ttttcatcaa taacagttcc
    77341 gtcaaaattt cctgaagtag caatattttt agtacgaacc atcgcaaact cctgcctgta
    77401 gttattgtta ttattccata tttccaatcc aatgctcttg aattcggcgg caataaactc
    77461 agcggccctg tcgatgtcgg gtgtaaaagc ccgccggccc cgcattgcat ctgaagcaag
    77521 tgtcttttca atcctttcca cttcctttac attaattact ttatcaatac ccggcaccgg
    77581 cgctgatgca gcattttctg ttgtaccaca gcttataatc aataaaaaag aggttgttgc
    77641 agtcaacaac cccattaaaa taaattttct catttgtaaa cttgacaaat taataaatga
    77701 acaactttta tattttaagc tgttcgctgc acaacccgct attcactata aactcagagg
    77761 ctcatcattt ctttgaactt caccgtttgc ctgcggctta cttcaatttt ctcgccccct
    77821 ttcatatcca gtaataaacc accgttaaaa tagggttcaa ttttttcaat cattctaagg
    77881 ttaacaatgt gcttacgatt agccctgaag aatatttttt catccaatct ttcctccagt
    77941 gcattcagtg atttcaggat taaaggctta ttgtttgcaa aaaaaacttt ggcatagtta
    78001 cccacactct caaacaacct gatatcactt agttttacaa accagcacct ttcaccatcc
    78061 ttaacaaaca cctggtcatt ttcggataaa atactattat tagtgaattc tgtatctgta
    78121 cggatctcct tattttctga aatctgtaat ttttgaatag catcggctaa acgtttgggt
    78181 tcaacaggtt tcatcaggta atccagcgca tttacttcaa aagcttttaa tgcaaattca
    78241 tggtgagcag tggtaaaaat cacatgagga gacttatcca attcagccag catatcaaac
    78301 cccgttttac cgggcatttg tatgtcaaga aatataaggt ctggttgagt cgcttctatt
    78361 ttggaaatag cctcatctgc attgacggcc tcatcaatta cttctatctc cggaaattcc
    78421 tgcaacaact ttcttaattc aagccttgcc aggcgttcgt catcgataat aattgctttc
    78481 atcgctgata tttttaatga atttcaaccg gtattaatac tttggcttct accaaagagc
    78541 cattaatttg ctttatagca aaacttgctt tgtcaccata cagcagattt aacctgttgg
    78601 tagtgctggg aataccaaac ccttctgtgt ttacagaacc attcagctga ccggtatttt
    78661 gaacaaccaa ctcatgatag tcacctttaa agtctgaaat tacttttatt acacctccat
    78721 tgatctgctg gctgatacca tgtttaattg cattttccac cagtgtttgc agcatcatag
    78781 gtggtactgg ctgatcaagg gtgtcttcat caatatcata ttctacttta agcctgtctt
    78841 caaacctcat attctctaat gccagataat ccttaataat atgcaattcc ttctcaagag
    78901 gtaccgtttc cattttttct gtctgcatgc tgcttctcaa aatattactt agttccgtta
    78961 ctgctttcct ggccctttgt ggattttcat caacaagagc cctgatactg tttaaagaat
    79021 tgaaaataaa atgagggttg atatgtgcct tgattgtctt cagttccagt tcttttacca
    79081 gcgactctaa ctttagagta tctaattgct gacgacggct cttattgtaa taatgataga
    79141 agtaatagat catcaaccag ggaaatataa agagaccatt atcaatggta gaacccagca
    79201 ctttttggaa aaaagatata ttttgttttg attcagtact aagattaaat acttctattg
    79261 aacctaaaat aagaacactg aacaaaatag atgtaaacaa tactgctata ataagccggg
    79321 gaagtgcttt ttcgaccggc aacagcatcc agccactcca tttaatgacc ccccttaaag
    79381 catgggtagt gattatacca gcaaagaaga caattgtaat tctttttaat agaataggtg
    79441 ttgaaatctg aacatcgtag atatgagcaa acagcaacat tacaaggcca accaggcccc
    79501 aacccccaac ctgtagtatc cagtaaagtt tatttttaga cattatagac ataaatatag
    79561 gagcaaaatt atagtttacc gctacccctt gtccctatct ttatatcaat ggcaaaaaag
    79621 ggctgtaaat ggaagattgg taataaaaac cttttcggga cgtttttgtt aaataatgct
    79681 attcgctttc ctctttgcca taaaacacta attttacatt cataaaatcc cagcatgcag
    79741 attacaccag gatcactttt acaaaccatt gattctccgg ccgatttgaa aaaactatcc
    79801 cgtgaaaaat tacaccaggt ctgtgatgaa ttacgacaat atatcattga tgtggtaagt
    79861 gttcatggtg gtcattttgg tgccagtctt ggagttgtgg aactaacaac tgcacttcat
    79921 tatgtttata acactcccta tgaccagttg gtatgggatg ttgggcacca agcttatgga
    79981 cataaaatac tgacaggccg ccgggataat tttcatacca acaggaagta caaagggatt
    80041 agcggcttcc ctaaaagaag cgaaagcgaa tatgacactt ttggtgttgg acattcctcg
    80101 acttctattt ccgcagcatt agggatggca atggcagcca aatacaaagg tgaaaaagac
    80161 agaaaggtag tggcggtaat tggggatggc tctatgacgg ctggtcttgc ttttgaaggg
    80221 atgaatcatg ccggcgtagc agatgcagat atgctgatca ttttaaatga taacggtata
    80281 ggcattgatc cgaatgtggg ggccctgaaa gagtatctta cagatattac cacatctcct
    80341 acctataata aagtaaagga tgatgtatgg aagctgatgg gcaaattacc ggtaggtaaa
    80401 aattttaccc gtgccatggc gcacaagctg gctgaaggaa tgaaaggaat ggtaagcagt
    80461 agcgccaatt tatttgaagc attgaagatc cgttatttcg gtcccataga tgggcataat
    80521 gtagccaaac tggtggatac actaaaagat cttcgggata tacctggtcc aaagatcctg
    80581 catattataa cgacaaaagg caagggctat gcgctggcag agaaagatca aactaaatgg
    80641 catgcgccgg gtttattcga taaagtaacc ggggaaatac agaaaaagat attcgatttg
    80701 ccacaacccc cgaaatacca ggatgttttt ggatatacaa tgattgagct ggcagaaatg
    80761 aatgataaga tatttggtat tacccccgct atgccctctg gttcatcatt aaaattcatg
    80821 atggagaaaa tgccagacag ggcatttgat gtaggcattg ctgaacagca tgctgtcacg
    80881 gttagtgccg gcatggctac ccaggggctc cgggtattct gtaatatcta ctcctctttt
    80941 atgcaaagag cttatgacca ggtagtgcat gatgtagcca ttcaaaaatt accagtggta
    81001 ttctgccttg atcgtgccgg tcttgttggt gatgacggcc caacgcatca tggctgctat
    81061 gatattgcct atttccgttg tatacccaat atgatcatca gcgccccgat gaatgagagt
    81121 gaacttagaa gcctgatgta tacagcgcag ctggaatcaa ccacacttcc ttttgttatc
    81181 cgttacccga gaggtgaagg tgttatgccc gaatggaaaa cagagatgaa agaaattgag
    81241 attggaaaag gaagaaaatt aaaagagggc aaagacattg ctattctttc attcggtcat
    81301 ccgggaaatt ttgcagcagc tgcaatccgt gatctgaaaa atgatggtct taatcccggt
    81361 cattatgata tgcgatttgc caaaccatta gatgaaactt tactccatga agtttgccgg
    81421 caatatcaaa aattggttac ggttgaagat ggatctgttg agggtggttt cggcagtgct
    81481 gttttggaat ttatggccca gcacggctat aagaacgatg taaaaattct cggcatacct
    81541 gataacctgg tagaacacgg tacacccaaa gagcttcacc gggagtgtgg ctatgatacc
    81601 cagggaatta ttgaaacggt gagagcaatg atgaaagtaa aggttacagc agaccagtta
    81661 ataagttaag ttctaagata aaaaaaagcc ggttactact aagttgatgc gtaaccggct
    81721 tttactatta attaaggata tcttatgcaa gattggcttc cattgtaatt tcagtattca
    81781 gcaatttact gatagggcaa ttagcctttg cttctgcagc acactcagca aacttagcag
    81841 catcaatacc aggtacggaa gcttttacct ccaggtgact tgatgtgata gcaccattct
    81901 ctaaagtgat tgtacacttt gtatcaatgt tatctggtgt gaagccagcg gcacccagta
    81961 caaaactcag cttcatggaa aaacagcctg catgtgcagc agccaccagt tcttcggggt
    82021 ttgtaaatgt tccatcttca aaacggctcc taaaatcata agaagtgttc tcaaggactt
    82081 ttgatttcga ggtcaaagta ccgcttcctt ctttgcctga ccctttccaa ttggcagatg
    82141 aatagctttt catgtgtaat agtttagcct cattaaaaga ggcggttaaa aaaattattg
    82201 ttgattttgt tcttctaatt cagcaatccg gttttcaggt tcattatatt caataatgaa
    82261 attattcttg ccttccccga tcatgatctt cataaacttc tgttgcacaa gctccagaag
    82321 tgataagaaa agaaaaatag catgaaccct gttttcacaa cgttcaaata ttttttcaaa
    82381 ggaaactgtc ttttcttccc gggccagatt cagcatctgg tcgcggcttt gttccatagt
    82441 gtaattatac gacactacag tatgtacagg cttattgatg cggtcgctgt atttctgcat
    82501 ggctttttca aaggcccgca ttaatttgaa aagagtgata ttttgaattt ctgttccttc
    82561 acccgtttct tccccaatct gtgacaattc cttctggata ttcccccttt tcaccataag
    82621 catccgcaat gcttccattt cagccatttg ggccgacgcc tctttaaacc ttttatactc
    82681 cagtatttta ttaatcaatt cctgtcgtgg atcaatttca ttgccttgtg catccagttc
    82741 cttgcggggc agcagcattt tggcttttat acgcatcagg gtggatataa aaagaataaa
    82801 ctcactgctg agctcaatat tcagctcttc actcttatga atatagtcaa gaaaatcatt
    82861 gataatactg gtaataggaa tattataaat atccagttca tctctctcaa taaagaaaag
    82921 caataagtca aatggtccct caaactgggg taatttaatc tggtacgatg tattctgcac
    82981 tgtaattggg tttgtgaaat aacaataaag tcctgttcta ccaacaggac tttacaaaaa
    83041 taagggaaac agggctcagg gaattcgcct gttttcccca aaattatttt ttcagtgcat
    83101 ccctgatttc acgcaacagc gtgatatcct caggtggggg tggcggagca gctgctgcct
    83161 cttttttctt agatgcatta acagctttta taaccatgaa caagaataaa gcaatggcta
    83221 aaaatttcac cgccgcagaa atagccagac cgtatttcaa ttcagcattt cccactttaa
    83281 cagacagggc atcgaaatta atgccccctg taatcattcc tacaacgggc attataatgt
    83341 tgtcgactac tgctccaaca atggcaccga aagcagcacc aataacgaca cccactgcca
    83401 ggtcaataac attgcctttc atggcaaatt ctttaaattc ttttagcatt cccataaaga
    83461 aatttttaat agttaataaa tcgtttcaga tgatccaggt tcaaaatttc caggttccta
    83521 tttttttaga ccaggaaact gcatatcaag atttttcagc aactcgtaaa gttgcgaagc
    83581 aattatatac tctttatacc aattctgatc tgaaggtaca actgtccagg gaggagtatt
    83641 acagttattg aaacaatctt catacatttt catatattca tcccaaagct tagcctcctc
    83701 aaaatcctta tcattatatt tccacatttt cgccgggtcc ttcattcgtt cactcagcct
    83761 ttcatgttgt tcttccggtg aaacatgcag gtagaatttt aaaatatgtg tgctattatg
    83821 tttttgcagc aatccttcaa aatcatttat agcttccatt ctttttcggg ctgtttcatc
    83881 atcacaccat ttatgaaccc tggttaccaa tatatcttca taatgagaac ggttaaatac
    83941 ctgtatcatt ccctttcccg gagcatgctg gtgaatacgc cataagaaat catgcgacaa
    84001 ctcttccgga gtaggagctt tatatgattt caccgtaacg ccctgcggat tcatattccc
    84061 caacacattt ctgattacgc catccttacc acttccatcc attccctgaa ttactatcaa
    84121 tattgaatgt ttgccttcag caaataaaag attttgtaat tcatccagtt caaccagaat
    84181 tttttctgtc ttttctttta attccttttt gtcaagatcc ttaggtgcac gggtatcaat
    84241 ttttttaagt tcaaccttat tcatagtatg tagtttaccg atatatgtct gcaatataaa
    84301 aaaattgcct ctgtctggct ctgttttatg acctttgccc acatttatga gtaataaatt
    84361 tatcagctgg gtgatcttcg taatcctgtg ttttatctgg ggaagttctt ttaaattaat
    84421 gaaagacagt gccattgctc ttaacgcctc acagatagcc tctcttcgca tttttgcagc
    84481 cggggtggtc tttatacctt ttgccttctt tcatttctct aaaatcccac gacaaaagat
    84541 gggcaccgtt attcttagtg ccgtatttgg aaatttaatt cccgcttttc tttttgcagg
    84601 agccatgaca aaaattgatg gctcactcgg tggtatactt aactcgctaa cgccaatttg
    84661 tgttgtagta ataggtatct tattttttaa ggacaagatt aaagcccaaa aaattaccgg
    84721 agtgattatt ggatttatcg gcctggtatt acttacgatt agtcctgtac ttatcggtga
    84781 aaaaaatata agctttgaca acatgaatta tttgtttctt atactcggtg ctactttact
    84841 atatggatta aatgttaata tggtgagtca ttatttaaaa gggctgaacc cgattcatgt
    84901 tgcaacagtt tcgctggctt ttatgaccat ccctgctgga ataattctct ggcaacagga
    84961 tgtcacacag cttgattttc acaagattga agtacggtat gcagtcatgg cttctgccgg
    85021 gcttggtgta ttgggaagtg ctatagcgac tgctttgttt tatattttag ttcaaaaagc
    85081 cggtggattg ttcgcatctt tagttacgta tggcattccc ttcattgcac tgttttgggg
    85141 ctttctggat aatgaaaaga tttctgtgat tgaaatcgtc tgcctgatga taattctctt
    85201 tggagtttac cttgctaaca ggtctgaaaa aaagaaagca acaccggcag atgttgcttc
    85261 ataaagattc atgtaaggta ttttataccg acataatttc tttctcttta gttgccaggt
    85321 gcttttctac agcagctatg aacttatcag ttatttgctg tatttcgtct tctgcatctt
    85381 tggcagcatc ttcactcaac ccgttttttt gtaacttttt aatatgttca atagcatcac
    85441 gacgaatatt gcggatggct acttttgaat gttctccctc tcccaggcat tttttaacca
    85501 attcttttcg tctttcctca gtcaaaggcg gtaaaaataa acgaatcagg acaccatcat
    85561 tctgaggagt aattcccata ttggctgcga taatagctct ttcaatgggt tggagcatat
    85621 ttttttccca gggctgaatg ctcagcgttc gggaatccat tacactaata tttgaaacct
    85681 ggttaactgg cacaggtgat ccataataat ccaccattaa gccatcaaac atctgcgggt
    85741 tagccttccc agcccttatc ttcaccaatt ctgcttccag gtgaccaatg gcttttttca
    85801 tgaaatcttc tgcgttactt gtaattgatg caatatcttc tgacataact tatagtttaa
    85861 aaaatcaaat ttactccggt acaattttat attgtaccaa gtcaggacac aaatctaatg
    85921 aaagaggtgt attcaaaaaa aatgccggca gaactttgtt atttgtttgc cggcacagaa
    85981 tacttttttt gcttcaatcc actgcagatt gggcttcttt tgtcagggtg actattttag
    86041 tagacgacgt ttttatttga gcgacccctt cacctctttt ggcaatgagt attgcacgcc
    86101 tgggcctctt gtaataatac aatatggcta acccggaaaa tgccagggtt agcataataa
    86161 gtaaaagata atttggtcct aatgatctgc caaaccaggc aatcatgata aaaccaatat
    86221 tagcgcttac acatgtaagc gttaccgaag catgatcaag ccccctgtcc agcaaaagat
    86281 gatgtacatg gtttctgtca ggcgtaaaag gagaaacacc attaaatatc cggatactga
    86341 aaaccctgag tgtatccaac aaaggaacaa ttaaaatcgc aaatccaatg gcaactgctg
    86401 aagaaaccgg gatagcaacc agcgggtcat ttgccacatt gataaattta attacaagga
    86461 tagcattgat caggcctatc atcagggaac ctgaatctcc cataaatatt tttgcaggat
    86521 gatggttaaa aatcataaag gcacctaaac ttccggccat ggcaaatgct aataatgcat
    86581 atgcctgaaa tccaatggca aagaaataac taccaaaaac aagcatggtc aggataccca
    86641 ggctggcagc cagcccgtct accccatcaa tcaggttaaa tgaattgatc acaacaatta
    86701 tcgtaagata agataatgca agggcaaaac cctcaggcaa attttcaaaa cccatcaggc
    86761 catacatact atctagcctg atacctccca ggtgaataag gatagaggca gcaattaact
    86821 gaccaataaa tttcttactg gcagataaaa taatgaggtc gtcctttagt cccaaaaaaa
    86881 agataactaa agcagccgcg aaaaaatact gaaattcaga attgagatgc ccctggattg
    86941 ataacaggga agccagaata aagccaccaa agataccaac accccctaaa gaggctacca
    87001 aacgggtatg tacttttctt tcgtttggca tgtcaaacaa cttctttttt tctgccactt
    87061 ccaatacaac cggtactgcc aggaaagtga taataaaaga aaccgaagct gttaataaaa
    87121 tatcgagcat ctaagcagtt ttatatggta caaaaatagg ctttaaccct tttggttttt
    87181 atattacaca gacattttaa agtttctgac attaattctt tgtaaccgat aggatgtgtt
    87241 gaatatattg tcagattaat ttaattcagt tggattttga atcaaaaaag aatgcaaatt
    87301 tgcaactaac ctgacctttg agggtgcaat gtactaaaag tttaacaaaa aactatttta
    87361 actatataag tatgaatgaa ctagcagcag tagcctctca aatcagaaga gacattgtaa
    87421 gaatggtcca tggagtcagc agcggtcatc cgggtggttc actgggttgt acagatttcc
    87481 ttactgcctt gtatttcaag gtgatgaaac ataatccagt ctttgacatg aatgctacag
    87541 atgaagatgt ttttgtactg tctaatggcc atatttcacc cttattttat tcaacactgg
    87601 cccgttccgg ctattttgac attaaagaat tagccagctt ccggaagtta aatacaagat
    87661 tgcagggcca ccctgctact catgaacatt tacccggtgt taggatagct tccggttcac
    87721 ttggccaggg aatgagtgtg gctattggtg ctgcattgac caaaaaactg aatggtaaaa
    87781 agaaccttgt tttttcctta cacggagatg gtgagctgga tgaaggacag atttgggaag
    87841 ccgtaatgtt tgcagcacat cacaaagttg acaacctgat ttcaaccatt gactggaatg
    87901 gacagcaaat agatggccca acaaataaag tcatgaattt gggaaatatc cgggaaaaat
    87961 ttgatgtttt tggatggaca actcttgaga tgaatggtaa tgatatggat gaagtggtag
    88021 ccaccctgga gaaagcgaaa tcgctgaccg gccagggaaa accagtcgct attatgatgc
    88081 atacagctat gggcaaaggt gtcgatttta tggagggcac tcatgaatgg catggcatag
    88141 cacctaacga tgaacaactg gctaaagcac tggcacaatt accggaaacg ctgggagatt
    88201 attaagagct tagattttat ataagcaaag gattattgga accccaaacc agtaatcctt
    88261 ttgcttttaa acctgataaa tcaagaaaac cttaattatt cacttcttaa tgaaaagttt
    88321 tgattggctg ctttccggga aggtaaccat gaagcaacca ttgcaatgat aaaaacggtg
    88381 gcgcctacca acaaaaaatc aatacccctg agttttacag gataataatc tatcagaaaa
    88441 gaacctccct gcaaagggat aagatgaaat tttatctgca accaggcaat caaaaaagcc
    88501 agcagcatac caattcctcc cccaataaga gccagtaaca ttccctcact taaaaaaatt
    88561 cgttgaataa aactccggtt accacccagt gcatgcaata cactgatatc tttttgtttt
    88621 tccagcacca gcatcgttaa ggcacctatc atattgaaag ctgcaacgac aaggatcagg
    88681 catagcactc catatacaat ccaacgttct atattcataa tggaaaaaag actacggttc
    88741 tgctcgtacc ggtcttgtat aagatacccg ttaccaaaaa ttcctcccag ttctttcctt
    88801 ataaccttca ttttcgaggg gtcttttagc gcaatttcaa ccccactata tacattcgca
    88861 gtaaggcgca gtgattttct tacaaagtcg atattggtta tgccatattt attatcaaag
    88921 tcctgctgta taagaaaagc tgccgatgac tgtatggtat cattgctgat atcctccata
    88981 atatctattt gtttggaatt actttttctt ggcagataga ttttcagcgt aaaaatattc
    89041 ctgtcagcat atatacccag cgcatcctca actcctgctc caagtaccaa cctgggaact
    89101 tcttcgttcc cgatattata ttcaccatta atcaaatgat cagccactcc ggttatataa
    89161 cggtaattct catcgacccc tttcaggtaa atcacagtct ggttatcccc attttgcacc
    89221 attgcttttt cttcaagtat catagaaaaa ttacggatac cagctactcc tttcagttgt
    89281 tttatttgct caggcgtgac agttatcatc ttacctgata ccggagaaac tttaagatcg
    89341 gtataaaaag aagagtagag agatttgacc agcccttcaa atccattaaa aacactcagc
    89401 accagaatca atgctgtagt accgataatt atagcagtaa tacttgtcca tgcaatgata
    89461 ttaatggcat tggtagactt ttttgcttta aaatatcgcc aggcaaagag aaaattcaag
    89521 ttgaaaaatt gagaggttta caagttggca agagttatta tccgttattt cccttctttt
    89581 cttcctcaat ctttttgaat agttcttcca ttttaaaaac atggtccagg gtgtcatcct
    89641 ggaaaaattt caaaaccgga atgctacgaa gctggtgccg cacttttgcc gccagctctt
    89701 tttttatttc atggtgacgg tcctcgatct tttttaatgc ggaacttata tcttttacct
    89761 ggaaaaaact caggtaaaat cttgcttcca gaagatcagg tgtcactttt accgatgaaa
    89821 tagacaccat tcccccgtca atcatattaa gaccgagccg ctggaaaata aaattcatct
    89881 cctcgttcag taatccggct atttgtttct gtcttttacc ttcttccatg ttttagtcat
    89941 tttttttgtc ttccggggca caagcatcgg atgacggcgt aaaattagtt actttgtccc
    90001 gtttacgcta ccaaataacg acatgaagca atattcaagg atttttcaat atctcggagc
    90061 ctataaagga aagatattcc tgtattttct ctctacactt cttagtattg ttttctcgat
    90121 cgtatctatt ggaatgttga tgcccttttt acaattgatc tttacaggca gccaaactgc
    90181 attagtggca gaaagcagca acccggtaat tcagtgggta aatgattttc tcaacaattc
    90241 tgttctaaca agaggcaaaa tagccaccct gggattgatc tgtctgctga tggtaagttt
    90301 cattgcatta aaaaatgtat tcctttatct atcgtactat gtactcaacc cattaaaaaa
    90361 cagaattgtt aatcaattac gggaagacct gtacgacaaa gtactccggt tgcctattgg
    90421 tttctttaat gaaaagagaa aaggtgacct gatgagtcgt atgaccaatg atgttagtga
    90481 agtggaaagc tccgttgttg gtacactgga aggatggatc agggacccga tgactattat
    90541 agtcaccctt accgtactct tccttatcag cgtacagtta actttattta ttctcatact
    90601 tattcccgta ttaggccttg taattggccg ggttaccaaa tccctgaaaa aacattcgca
    90661 ggatgtagcc aataaatatg gtgaaacact ttcaacactc gatgaaacat tgggagggtt
    90721 aagagttatc aaagctttta atattgaaaa agtattacga tcgaagtttt ttaaaagcaa
    90781 tgaagaattg ctgtattcaa aaaataaaat cagctaccgg agagatcttg cttccccttt
    90841 atctgaagtg atgggagtgg tactttttac tgctgtattg tattatggcg gacgtttggt
    90901 tttacaaaat caattattgg aagcatctgc ttttttaggc tttctcggta ttttctataa
    90961 tattataaac cctgccaagg cattatccac ttctttcagc aatatgcgga aaggggctgc
    91021 tgctataaat cgtattgaag aagtactgaa aacacccatt actgtagacg acaatccaaa
    91081 agggagactt cttcctgctt ttacagacaa gattgaattc aggaatgtaa gttttgctta
    91141 tgacgatgct atgatactgg ataacatcaa tcttaccgtc aaaaaaggcc aaaccattgc
    91201 actggttggt tcctccgggg ctggcaaatc cacactggcc gacctggtac caagatttca
    91261 tgatgtaaca ggcggtgaag tactgatcga tggcatcaat ataaaagact actcgttaca
    91321 ttctgtacgc agcctgatga gtatcgtaac acaggaacct attttattta atgatactat
    91381 tggcaataac atcgctttag gccagcaggg cgcaacagaa gaacaaattc atgaagcagc
    91441 aaaaattgcc aatgcccatg agtttatcat caaaaaagag ggaggatata acagtaatat
    91501 cggtgacagg ggcagcaaac taagtggtgg ggaaagacaa cggttgacca ttgcccgtgc
    91561 tgtattaaaa aacccaccga tcctgatcct ggatgaagcc acctcttctc ttgatactga
    91621 aagtgaaagg ttggtacagg acgccattaa taatatgatg cagaaccgaa ccagcattgt
    91681 cattgcacat cgcctctcga ctatccggca tgcagatgag atcattgtat tgcaaaaagg
    91741 aaaaattgtt gaaagaggaa accatgaaca gctgctggca caaaatggtt tctataaaaa
    91801 gctggtagat atgcaggaag tgaaataaga ctttacttaa acagcgccac taagccataa
    91861 ttttttacag gttcacctta taaagcttta ctgaatatta cttcaaaact tctctcgata
    91921 taaccagctt ctgtatctca cttgtaccct caccaatagt acaaagcttt gcatcccgat
    91981 aatgtttctc taccggaaaa tctttggtat aaccataccc gccaaatacc tgtacagcat
    92041 catttgctac tcttacagcg acttcactgg catagtattt agccatggct gcttctttgg
    92101 tcatcggcag gtggcggttc tttaaatcac aggcttgtaa tgtcagaagt tctgctgctg
    92161 caatttctgt ggccatatct gctaacttaa acgatatgcc ctggaaagag gaaatcggtt
    92221 tatcaaactg gtatcgttcc tgcgaatatt tcaatgccgc ttcatatgca cctttggcaa
    92281 tacccaatga aagagaggcg atagaaatcc ggcccccatc caatactttc atggcttgtt
    92341 taaaaccatc tcccacttca cccaacctgt tcgtatccgg tatacggcaa ttatcaaaga
    92401 ccatttcagc cgtttcactg gcccgcattc ccaatttatt ttcttttttg cctgcactga
    92461 aaccagctgt tcctttttca actacaaagg tggtggagtt gttttttgct cttggttcac
    92521 cagtacggca gatcaccacg gcaacatcac cactcttacc atgagtgatc cagtttttgg
    92581 taccgctgat cacccaatca ttaccatctt tcactgctgt gcatttcatg ttacctgcat
    92641 cactgccggt attagcttcg gtcaatcccc aggctcctat ccattcagct gtcgccaatt
    92701 tcggtaacca ttttttcttt tgctcctcgc tggcaaacga taatatatgt cctgtgcaca
    92761 aagaattatg tgctgctaca ctcaaaccta ttgatccgca aactttggct atctcttcaa
    92821 taattgtttt atactcaaaa taagtgagtc cggcgccacc atattcatgc ggcaccagta
    92881 cacccatcat acccagttta cccagttcct taaatatatg aactgggaac tcctggcttt
    92941 catcccactc catcacatgt ggtttgatat gttgttgtgc aaaatctttt gctgtctgtg
    93001 cgacctgttg tgtaagttca gctgactgaa aattcatatg tatttaaatc gttgttattg
    93061 tcatttaata actgtgccac tcccggtaag gggtggcaca gtttaggcct tgagatcaaa
    93121 agcaagcatt cgttaaaggc aggtgcaaga taatggaaat ttcacaaaac taacaagtgt
    93181 tagtgcaaag aaatttttca actatgcctt cttatttaaa atcaagttcc aatccatcgt
    93241 aagccaggtg gatattatca ggcaattcag cttcaatttc agcatgtagc ccgatctggt
    93301 gtgacatatg ggtgaaatag gctgtcggaa tttttaattc ctgcactact tcaattgcct
    93361 cttcaagtgt gaaatgggaa atgtgctttt gtttgcgaag ggcatttaat actaatactt
    93421 cactgcctct tattctttcc aactctttat catcaatccg gttagcatca gtgatataag
    93481 taaacttacc aaaccgaaaa ccaagcaccg gcatctttaa atgccatacc gaaaccggtg
    93541 tgacaggaat atctccaacc acaaatggtt cagaagaaat cgtattaagg tttaattcag
    93601 gtatacctgg atacttggta tcagtaaaag cataataaaa atctcttctt acagcttctt
    93661 ctgtcagtga atcagcataa atgtctatat gccttttagt aaaaaaatta taagccctta
    93721 cgtcatccaa tcccgccagg tggtctttgt gtggatgggt aaaaactacc gcatccaatt
    93781 ttttaacctt ctggcgaagc atttggtaac gaaaatcagg acctgtatct accaccaaag
    93841 ttgttttggc tgactggaca agaatacttg aacggagccg cttatctttt tcatctgaag
    93901 aagtgcaaac ctcgcaatca caaccgatca taggaacacc actgctggtg cctgtaccaa
    93961 gaaatgttat ttttaaagga gggcaaatca cagggtaaag ttaattagtt aatcaggtat
    94021 caggagagga ttcaattaac ttaatccccc tgattaaaaa attactctaa ttaccatttt
    94081 cctccttctc gagtttggaa atgatacctg tatacaattt tttggactcc tctgtcagta
    94141 attcagtatt aatctccaac tgtgggatca ggtctatcag cgtattgatg cggccttcga
    94201 tttgcaaaaa cttatttaat acaaccactt ttttggattc aagtatacaa taaccgctct
    94261 ggaaagtacc cctttcatag cgaagtacgt aacccgcctc ctctggtatt gcttctaatt
    94321 tatctagggt ggtttgagta tatttcatag taaatagtca aaagtgattt ttttcaaagg
    94381 taaattactc ttgacggcga aatggctact atcaattaat attttccaca tcgcattaac
    94441 tattcataac agccacttgc ttacctgctc tgcatcttta taaccggaac tatcatttcc
    94501 tccaggctca ctccaccatg ctgaaaagta ttacggtaat aattcacata ataattatag
    94561 ttgttggggt agcacagaaa gctgtcttct tttgcaaaaa tgaaagatga gttaatagtg
    94621 ggtaccggca aacccgcctc tcttgggtca cggaaagcta atacctcctt tggttcataa
    94681 ttcagattac ggccatgttt gtagcgaaga ttcgtggttg tttgtttatc tcccaccact
    94741 ttaaatggtg ttttcactct tacactgccg tggtccgtag ctaatactaa ctttatattc
    94801 ttatcagcga ccttttttaa agcctggtgc agtggtgaat gctcaaacca ggaagccgtg
    94861 atgctacggt aactcatttc atcaccggcc agttccttca gcacttccat ttctgtacgg
    94921 gcatggctca gcatgtctac aaaattgtag acaatgacgt tgatatcatt gttcaggagg
    94981 ttatgaatgt tatttaccag gtccaagcct gcctggttgt tcactacttt ggtatatgat
    95041 actttcaggt catttttacc taatcgtttc agctgagccc ggaaaaattc ttcttcatgc
    95101 aaattttttc cgccttcttc ttcatcattt ttccattgca acggaaactg tttttcaatt
    95161 tcaaccggca ttagtcccgc aaagatggca ttacggcaat attgtgtagc ggttggcaat
    95221 atgctgtaaa aactttcttc ctccagtatg cggaagcttt cagcaaaaat gggttgtata
    95281 gccttccatt ggtcaaacct taaattgtca atcaatataa aaaagagggg tgtccctttt
    95341 tccagatgtg gcagcacttt aaattttaat aatgtatggc tcatcaccgg gccatctgta
    95401 ctttttggcg aaacccagct ggcgtaattc tttgaaacaa atttaaaaaa ctcagtattg
    95461 gcttcgcttt tctgattttg cagtatctcc tgcatttccg ggctgtcact cttttccatt
    95521 tccaattccc aaaacaccag ctttttataa atatccatcc actcattata atcagggtta
    95581 ctattcagtg cagtaaagag atggcgaaac tgctgttgat aagccgtagt ggtcttttct
    95641 gctaccaatc ttctgttatc aattatcttc ttcaaactca accatacctg gttaggattg
    95701 accggtttaa taaggtagtc actaatctgc gaaccaatcg catcatccat caggttctca
    95761 gtttcatttt tagtgatcaa tacaaccggt aacgattgat ttatctcttt aattttagct
    95821 aaagtctcta acccggtaat acccggcatg ctttcatcta tcagtaccac atcaacagga
    95881 ttttccttca catagtcaat cgcatcaaac ccgttagtaa aagtttgtac ttcatatcct
    95941 ttattctcca gaaaaagttt ctgagattga aggctttcta tttcgtcatc tacccagatt
    96001 atttttgcta ctgccattct taagattgat taaaagtgtg acctgtctgc caataaggcc
    96061 tgaatgtggt aaaaatagct ttttaatttc tccctttgta catttgccgc ctgccctttc
    96121 cgggccttta acaaaacccc aacaatgccc agcgtccgca aaatcataaa cgacccggta
    96181 tatggtttta ttaccattga tcatccgctg atattgcaaa ttatctctca tcctgtttac
    96241 cagcggttgc gtaatattca ccagatggca tttgcacacc tggtatatcc cggtgccgta
    96301 cattcgagac tgcaccactc cctcggtgcc tatcatttga tgtgcatggc actgggcgaa
    96361 ttaaagagta aaagcattga aatttctgct gaagaagaac tagcagctaa agtagcaatt
    96421 ctattacatg atattgggca tggccccttt tcccatgcac tggaaaatga gttgataaaa
    96481 aatgtacacc atgaaaccat ttcattgttg cttatgcaaa aactcaatag tgagttgaac
    96541 gggcagctgg caattgctat tgaaattttc accaacaaac attctaaaaa atttctccac
    96601 cagctggttt ccggtcagct ggatgtggac agaatggact acctcaaccg ggatagtttt
    96661 tttaccggtg tggccgaagg agtcatcggc tacgatcgta tcatcaaaat gctgaccgtg
    96721 aaagacggta acctggtggt agaagaaaaa gccatttatt ctattgaaaa attcctggta
    96781 agccgccgtc ttatgtactg gcaggtgtac ctgcataaaa cggtggtaag tgctgaaatg
    96841 atgctcgtaa agattatccg gcgggccaat gaactgattg caaaaggtga aaaagtggct
    96901 gcagcatcca atgaacttga tttcttttta caaaattcag aaccagattc atcaatcgag
    96961 cagcatttag atactttttg ccgtctggac gaccatgatg tgatggctac aattaagaat
    97021 tggtgccatc accaggataa aatattggcc acactatgcc gttatttagt ggaacgaaaa
    97081 ttgctgaaag taaaattaca ggccgaacct tttgatgagc aatttgtaaa tcagaaaaaa
    97141 aaagaagctt gtattaaact tgagatcagt gaaaaggaag tggattattt tgtatttacc
    97201 ggtgaagcca gcaatacaac atataaccca ggggacgagc ggatcaatat tttattcaaa
    97261 gacggatcaa ttaaagatat ttcaaaagta gataatgcac tgattcagca taacctgagc
    97321 ggcactgtta aaaaatatta catttgttat atgagatagt tgagcgttga gagccttgag
    97381 ctatacgcct gaaaaaccag gctgttgctc aatctcagtt ttaaaactta aagcttataa
    97441 attcatgcaa ttccctgctg cacaaatagc cctaatcgtt aagggtaaaa tagaaggcga
    97501 tccaaataca acggtaaact cttttggaaa gattgaagaa gccaaagaag gtcaacttag
    97561 ttttttagct aaccccaaat acgaagatta tttatacagc accaaggctt ctgtgattat
    97621 tgtcaatgaa aattttgaag ctaaacaacc cgttaatgcc acacttatcc gggtgccgga
    97681 tgcgtataca gcattcgcta cattgctcag taaataccag gagataaaac agcagcaatt
    97741 aagtggcatc cagcaaccag tttttattgc tccaacagcc acttatggcc aaaatgtctt
    97801 catcggtgca tttgcctacc tgggagaaaa tgtagttgtg ggaaataaca ccaagattta
    97861 tcccaatgta tatgtaggag ataatgtaac gctaggcgaa aacagcattt tacatcccgg
    97921 tgtgaagatc tatcatgatt gtaaagtggg taacaatgta attatccacg ccggaactgt
    97981 tatcggcagc gacggttttg gttttgcgcc acaagcagat ggaagtttta ttaaagtgcc
    98041 gcagatcggt aatgtggtga tagaggataa tgtagaaata ggatcaaata ctactatcga
    98101 ccgggccact attggctcca cattgatcaa aagcggtgct aaactggaca accttattca
    98161 gatagcacat aatgtagaag tgggaaacag tacagtcatt gccgcacaag cgggtatcag
    98221 cggcagtact aagattggaa atggtgttat gattggaggc caggccggcc ttgcaggcca
    98281 ccttcatgtt gccgatggtt ctaagatcaa tgcacaggcc ggtttaggca aatcgctgaa
    98341 aacgcctaac accgctgtta ccggatcacc ggcttatgag tataacaagg cgatccggag
    98401 ccaatccatg gcacgcaact tacctgagtt agagaaaaga gtaaaagaac tggaaacaat
    98461 aataaaacaa cttttagcag aaagagttgt ataaaaatag ttgtacagcc ttcagcttct
    98521 cagcaatcct tagtaataat tcctaatttg ccatcatgag aaaaatttcc ttgtcgcttt
    98581 cttttttctt tcttgcggta accattctag cacagcaaaa accagcttat attttatata
    98641 atgccaaagg ccaacaaata acgtataaga aaatgctgaa aacgcttcag caaaaagaca
    98701 tagtactgtt tggtgaattt cataataatg ctattgctca ttggttgcaa ctggaagtaa
    98761 caaaagattg tgatgctacc cgtgacctgg tactcggcgc tgaaatgttt gaacaggata
    98821 accagcaggc actggattta tacctgcagg gtaaaatcac tgcaaaaggg ctggactcca
    98881 tggcccgcct ttggaaaaac tatccgactg attatgcccc tctggtgaat tacgccaaag
    98941 aaaataaaat agcttttgca gctacgaata tccccagacg ctatgcttcc ttagtatcca
    99001 aaggaggctt tgaaacactt gatactttat ctgcccaggc caaaacatgg attgctcctt
    99061 tgccaatagc ttatgatgcc gaactgccag gctataaaaa aattaaagaa atgatggctg
    99121 gccatggcgg cgacaatatg cccaaagccc aggccagtaa agatgctaca atggcgcatt
    99181 ttattttgcg gtattatcag cccggaaaac ttttcattca ctataacgga gcttttcatt
    99241 cggagaacta tgaaggcata ttgtggtatt taaaaaaaca gcaacctgaa ttgaaatacg
    99301 ccaccattac aactgtgtcg caaaaagata tccattcgtt gctggcagaa aataaaggga
    99361 aagctgattt tattatttgt gtggatgaag acatgaccaa cacttattaa tcaccctact
    99421 tcatgattat ccatggcttt acatttgttc gcaatgctgt gaaatacgac taccctgtta
    99481 ttgaatccat tacctctatt cttccaattg ttgatgagtt catcgtaagt gtaggcaata
    99541 gtgatgataa cacccttcag ctgattgaat caattgattc gccaaaaatc aggatcattc
    99601 actctgtttg ggatgattcg ctgagagagg gtggtcgggt actagccgtt gaaactgata
    99661 aagcttttgc ccggatttcc ccatctgcag attgggcttt ttacctgcag gcagatgagg
    99721 ttgtacacga gaagtatctt gagacaataa aaaatgaggc tgagaaatac cttcatgata
    99781 aaaaagtgga gggattgctt tttaaatatc ttcattttta tggaacatac gattatatag
    99841 ggaattcccg tcgctggtat aaccgggaga tcaggctgat ccggaatgac aaaacaatca
    99901 aatcctaccg ggatgcccag ggatttcgta aggcaggccg gaaattaaat gtaaaattga
    99961 ttgatgccta tattaatcat tacgggtggg tgaaacatcc ccaaaagcag cagaaaaaaa
   100021 gggatgactt tggattacta tggagcaatg accaacaggc gattattaaa gaagagacca
   100081 gggacatgtt tgattatatg aaagatgcag atgtattaat gccttttact gattcgcacc
   100141 ctgctgtaat gcataaaaga ataaacgaga aaaactggga agcaaatttt aatctgacca
   100201 aaacaaatct ctcagtaaaa gaccgtctcc tttataacat agaaaaaata acaggcagga
   100261 ggatatttga ttaccgtaat tacaagatca tctgacatag ctgtatgact gacggttaaa
   100321 tactatcttt ggccgcataa aaaatacagc atggctccag atttcaatcc agataaacaa
   100381 catacgctga aatcgccggt taccatttca ggtacagggt tacataccgg tgtaatggct
   100441 gatttaacgc taaacccggc aaatcccgga tttggcttac agtttaaacg tatcgattta
   100501 cccaatcagc ctattatcaa agctgattgt gacctggtaa cagatgtaag tcggggtacc
   100561 acactggaag ccaatggtgc caaagtaagt actattgaac atgtacttgc tgcattagta
   100621 ggaatgggag tagataattg cctgttggag ataaacggac cagaaatgcc tattatggat
   100681 ggcagttcac aaccttttgt ggagttgatt gaagaatccg gtgtgctgga gcaggatgct
   100741 gctaaagtct ggtatagtat cgatgaaaat atttaccatt acgatgaaga gaaaagagtt
   100801 gaaatggtag ccatgcctgc catggaatac caggttacaa cattgattga tttcaactca
   100861 cctgttttag gtacccagca tgcaggactg aaaaatatca gggactttaa agaagaaatt
   100921 gctccttgcc gcacattttg ctttttacat gaactggaaa tgctgctgga taataatctt
   100981 ataaaagggg gtgacgtcaa caatgcaatt gttattgttg acaaacgggt ggatgacaaa
   101041 gaaatggaaa ggctgaaaaa aatattcaag aaagataaaa ttgaggttaa aagcgaaggg
   101101 tacctgaata atcttgaatt gcgatttccc aatgaacctg cccgccataa attattggat
   101161 gtaattggcg acctggcttt gataggctat cctattaaag gaagaatcat tgccaatcgt
   101221 ccgggacata gcagcaatgt agattttgcc aaaaagataa agcagtacat caaaaagaat
   101281 aagcacacta aaggtgtacc tgtttatgat ccttcgcagc cgcctgtgta tgatcagcag
   101341 tttattgaga aaaagttgcc acatcgtttt ccttttgcac tggtcgataa aataattgaa
   101401 ctgagtgata cgcatatcgt tggtattaaa aatgtcactt tcaatgaatg gtttttccag
   101461 ggtcattttc ccggcaaccc tgtaatgccc ggagtgctgc agatagaagc attggctcaa
   101521 tgcggtggta ttttggctat caacctgagc ggggaaggac aatacgatac ttactttctt
   101581 aaaattgata actgtaaatt caaacagatg gtaagaccag gggatacttt gatcctcaaa
   101641 atggaattat cagcgcctat ccgccgcggt atttgtgaaa tgaaaggcac aacatatgtc
   101701 gggaataagg tatgcgcaga agcagacctg gtcgcccaaa ttgttaaacg ataacaacaa
   101761 taaaattcat taaaaaaaca cactgatttt ctgatggtaa gaatcagctg aaatgcccga
   101821 aaatcgggca ttttcattta tccacaataa cccaaaataa gtgtacaaaa tagcctctga
   101881 aacccggata cacggcttca aatccgtaca tttgtgagtt atattttttt cacttttaac
   101941 gacaggattt aagttatttt atgagtccag aagccaaaga aaaagcagcc agaatttcgg
   102001 ccaattacag tgcagacagt atacaggttt tagaaggttt ggaagccgtt cggaagcgtc
   102061 cggcgatgta cataggcgat attggtgtta agggtttgca ccatcttgtt tatgaagttg
   102121 tagacaactc aattgatgag gccctggcag gttattgcaa aaatatcact gtcaatatcc
   102181 atgaggacaa ttctatttct gtagaagatg atggccgggg tattcctacg gccatgcata
   102241 gcaaagaaaa aaaatctgct cttgaagtag taatgaccgt actgcatgcc gggggtaagt
   102301 ttgataaaga ttcatataaa gtgtccggcg gtttgcatgg tgtgggtgtt agctgcgtaa
   102361 atgccttaag taaaagactt ctggtaacag taaacaggga tggaaaaata tttgaacagg
   102421 aatacaagat cggtgttccc caatatgctg ttcgtgaatc tggcaccagt gaaaccaccg
   102481 gcaccaaagt acatttctgg cccgatgaca gtatcttcat taccaccgtt tataataagg
   102541 atattttaga aagccgcctc cgtgaactgt ctttcctgaa caggggcgtc cgtattatac
   102601 tcaacgatct gagggaaaaa gaagaagatg gcagcactta taccaaaagt ttttatagtg
   102661 aaggtggtat agtagagttt gtggaaatgc ttgacaaaaa tgctggtcgt aactctctca
   102721 ttcctaaaat aatttctgta gagggcagag atgaaaacac aaatgttgcg gtagaagtag
   102781 cattaagtta taatgacagc tatagtgagc atatctactc ttatgtcaat aatatcaata
   102841 ctattgaggg tggtacccac gtatccggct tccgccgtgc gctgacaaga gtatttaaaa
   102901 gttatggtga taagaatgga ttatttgaaa aagctaaagt tgaaattgaa ggggatgact
   102961 ttcgtgaagg cttaagtgct atcatctccg taaaagttcc cgagccccaa tttgaaggcc
   103021 agacaaaaac aaagttgggt aataacgaag taatgggtgt ggttgattct actgtatcca
   103081 aagtattgga ggcatacctc gaagagaatc caagggaagc taaaaacatt gtatccaaag
   103141 ttattcttgc cgcacaggca agggctgcgg ccaggaaagc cagagaattg gtacaacgta
   103201 aatctgtttt aaccggtggc ggattgcccg gtaaactggc cgattgttct gaccgtgacc
   103261 ccgaacgttg cgaattattc cttgttgagg gagactctgc cggtggtacg gccaagcagg
   103321 gccgtgacag aagtttccag gctattcttc ctttaagagg taagatcctg aacgtagaaa
   103381 aagcaatgga acataagatt tatgacaatg aggagatcag gaatatgttc actgccttag
   103441 gcgtaaagat gggaacaccg gaagatccca aggctttaga cctgagtaaa ctccgttatc
   103501 ataaactcat catcatgacc gatgccgatg tggacggatc tcacattgcc acattgatct
   103561 taactttcgt ttaccggtat atgacagaac tggttgagca aggctacgtt tatattgcac
   103621 agccaccgtt atacctggtg aaaaaaggaa aagaacaggc ttatgcctat aatgagaacc
   103681 agcgcaaggc tcttattgaa aagctgggtg gtggcaaaga tgattcagtt aatattcagc
   103741 gctacaaagg tttgggagaa atgaacgcag aacagctttg ggaaacaacc atgaaccccg
   103801 gaagccgtac cttaaaaaga gtaactattg aaagtgctgc cgaagcggac cgcattttca
   103861 gcatgttgat gggggatgaa gttgccccca gaagagagtt tattgaaagc catgcaaaat
   103921 atgcgaaact ggatatttaa gcccgggagg cttaatatgc tcctgtttta tcaattctac
   103981 tacaaaatat ataactaatt catagcaaat gttttagccc tgttaaatca gggctttttt
   104041 atgcctgttt ttaaaattcc accttcaaaa agccccggaa tgcttaattt caatctttac
   104101 taacgcaaaa acttaaacca tgtctgatgt aaaaattaca gcctacaatg taaaaaccaa
   104161 agaaaaaggt gtagaaatgt ttgatgccgt tatcacaaaa acagccaagg gtgcttatat
   104221 ggcacagggc aatgacggaa agggcaataa actcacaacc ctgatgggcg aagaaaaagc
   104281 aatggctgcc atcaaagccg gagtagcaaa acaaggctgg taaaacaagc ctttaaaaaa
   104341 tatacccgtc ccgcccctct ggcgggacgg gttttttatt gtttaatgag tttgaaagag
   104401 tgggtctttc cgcctgtcat tagttccagc atgtacagcc cggaagaaaa tttagcatct
   104461 gccaggttga tagtgagctt ctgaacaaaa ccattggaaa catgatcaaa tcggttcacc
   104521 accctgccat taatatcata gactttcaca gaaatgggct ccccatcgtt ggcctggtaa
   104581 gtaagattaa atatgccgga agacggattg ggagtaacca accatttgag ttcatcatta
   104641 aatacaacag gacgtattgc tgaataactg aagctgccgt cattctcaat aatctttaag
   104701 cgataataac gaacaccatt cttattattt tcaatatcag tgaagttata gcgttgctcc
   104761 ctttctgaat taccctgact tctaacctca cctattttca taaaccggtc ctgttgaaat
   104821 tctgtattgc cctttgcgac ttccaaatca aaacgattta cattaaactc tgaagtagta
   104881 acccattccg caagaacatc ttttacattg gctttcctgg ccgtaaagct taccaactgg
   104941 actggtaagg gctgattatt atcaaagccc ccattattga gccagaactc tgaaaagtta
   105001 tttactttaa attcggcata ataaccttta tcaaaaggca cttttgttgc cctggttgaa
   105061 ttgataaatg tccagattcc ctggttatta tctgctacac tgccattttc aaagttgtta
   105121 tccggatcac tgtactttga aacacccagg tcgtaggcag tggatggttt ataacaatag
   105181 ctgcaacccg atgcattgat caaagcttct gtctcagaat caagaaagta ataacgcacc
   105241 agcacagaat ctgttaatga caggaatgaa ttacccggct gaatagtaat attccggtcg
   105301 tgataaaact ggtcgctgtc tattcttact gctccagtat ttatataagc ttgtgcgttt
   105361 gttacaccca attcctgccc ggcaggatga atggaagcaa cgagtttgcc tccttccaga
   105421 aaatcaaccc agttgtttat tccggcattg atcgtttgcg taaccggtga tgtcatggta
   105481 accccatcat aaattccatt tagattatca taaatatgaa tatcatcaat ggcaagacca
   105541 tctttgctca atgattcatc ggatttaaat acaaaacgca ggcggagttt actattgttg
   105601 gtagttggca gcgctgttgt agccacatgc cagcgggtat agttttgctg gctccatagt
   105661 tgattgcctg catagtcttt attataccaa ttagtgcctt gcccagtact gcctagcttt
   105721 gtccatactg ttccgccatc cgttgaatac tccacccagg ctgcatcaca taaaacagct
   105781 ccgcaatcct caatatccat ggcaatgctc agacttaatg tcggggcggt cattcccgag
   105841 atattaaagc agggtgaata gagataagaa tattcagcat cattataata tccggcaatc
   105901 ctggttttcc atgctttact gccactggca gcccggttga tcttaggtga aaccggtgtt
   105961 ccatattccc aggaagttct tgcacctccc gcataccagg aaccattacc caattcaaaa
   106021 ttctgcagat aagggaatgt agttaccatc ggtaaattat tgataaccgc ccttgcagtg
   106081 tcattctccc tgtagttatc cgtaataagt gcgacccata cttctatgtt atgatctcca
   106141 acagcagaaa gatcagcagt agcggtaaat gtgtactgaa ttgctgagct ggcaggaata
   106201 gtaataattg cttcagtagc tattatattt ccatcaactt tcaaaacaac cggtatatta
   106261 tttatcactg cattagaagt attgcgaact gtaaccatta caggcaccgt tgcatctaaa
   106321 ccacaactca cagcataagg agtatcaata ctaactaatt gtatatcatc gacagctttg
   106381 tatatacgta catcatcaaa tgtataacca gctgcacttt cattatcagc tgccaggtat
   106441 tctccccact gtccccaccg tgcctggaaa cttgtagaaa aagtttgcgc atgagccgac
   106501 aatgtatcgc tcacttcaat actgccagaa agcttgtaag aaccatctgc aggattttga
   106561 tttgcaaaaa ggtcatacac ttcgatccag ctgtccgttt cgctgccccg gatccacact
   106621 ttatttgcag catttgattt ttggccatga ttcttgtacc tgaagtccaa tcttatatca
   106681 tcagtagctg tattaaatgt tgccaggtta aaagtacctg tcagtgaatc aatatttcct
   106741 ccggcaacat acctgtcctg atccagggta agagcttttg ttcccgagta agcgattcca
   106801 gtattaataa aagttctggc ttgcccgtac accgttccat ttacaaagtc atacctgtca
   106861 aggccatcaa gacccatttg aacatttctc acagtttgta cagttgcaga ttcaaaatta
   106921 tccagccagg ggagctccgt atttgtgata ggttgattat ctaactgctt aaacatttta
   106981 gtgaaactat tatttgtaat aaccggatca gtagcttttg cagctgttac ttgcacactg
   107041 taggtaccaa cgccggaaag attagcaggg gtgataaaag tataatcaat tgttccccct
   107101 gcagctatat ccgcaacagg tgatgttatt atttcattta cagctgctcc tccattaata
   107161 gtataactaa cattaatatt ccctgctgaa gcagcatcat ccaggttttt gatccggata
   107221 gttatagatt cattatttga caatgctgaa gcagtaaaaa tccttccgga tgaagcaggc
   107281 gcaacaattg catcaatctt caggtcatta tcggatatcg ttccggtgca agtcccatta
   107341 tttggttgac gggaaacagc attggctctt ctgcccgtac taccattgat tcttggtcgt
   107401 acggttaccc agtataaaga atcttttgat aaaccactaa acgtgtaagt aagtaatgga
   107461 gttgtagcta ctgaaatcat ttcatccccc tgtaacatca tcacttcata atctgtagca
   107521 ccattgacgg ctgtccagtt aatttttatg tacccttcac attgtacagg atctaaagaa
   107581 actgtactca ttgctgttat ggtgaaagga aaacttgttt gtgtaaaagc agtgctattc
   107641 tttgtaattc ttattcttgc ctggtcagtt ggagtattgg gtacacccca gttataaatt
   107701 cggctggctg caggtatggt attactcaat gtagtccagt tagtcccatt atcacttgaa
   107761 aattccaatg taaaatcatt ggcagtacct ccatacgaat cccattgaat ataacaggta
   107821 tcaaaagcag atacggaagg caccaatgat tctcctccaa caggatttga aagaacgagg
   107881 ctttcaggaa tcagatcata caccaggaaa tattcctgcg aaggattctg tgtaatagct
   107941 gatccgatta ctcttatatc ataagttcct gcagccgggt tatcaataac tacctgttca
   108001 atattattga tatgatcggc tcctgttgtt gctgcattgt ttacgttggc gggaattgta
   108061 tccaaaataa caggtaaaac aattccactt gatggagtga ccacttcaat atctatatca
   108121 tttaccaaag tgcttgaagc cattaccgaa gcaggaggat cctgccaata gactaaaacc
   108181 ttaagctgag ctgtattggc cggaacaatt acagcatgcg tattggttga tgaatttgta
   108241 accgttgaat taaaataagt tgcattttct atcgtggcaa cagaacgtaa caggttcatt
   108301 cttccaaagc catacctaaa gtcaggcccg ggattccctt tgtcatctcc cccattacaa
   108361 agcaaggctt tcatcaaccc gcttttagga ttggccccgg cattcaattg acggtatcgt
   108421 tgaattaaca atgccaggcc accggatact cccggagcgg ccatgcttgt accattatta
   108481 taactataca agttgcttac ccaggtggaa gccacaagct gaccctgtga catgatctcc
   108541 ggtttcaatc taccatcccg aactggccca cgactggaaa agccactgat atcactttta
   108601 taatctgtac tcccaactgt caatacattt tttgcggact gatagccacc caatacagtt
   108661 ttaaatcctg cgggatatgg ggcacatgtt aaccccccat cattgccagc tgcaaaaacc
   108721 tgctgcaact caggaaggtc aaaagcctgc tgatccagaa tacgggagga aagatcatac
   108781 aagccgttat aaaaacaatc agttactaca gtcccaaatg aattattggt gatcaccatt
   108841 ccatgatcct gtacatacgc cggcgcatta gcaaaaatgc ctgaaaaata ctggttaatt
   108901 attgttgctt tgggagcata cccggcatag cgttcctgaa ttattcctgc tccggcaaca
   108961 gtaccagcca catgggtagc atgagctctc attacttcac ctgcccggtt tattaaccgt
   109021 ccagtgaaat caagatgcga ctgcacatcc ccattatcgc caataccgat tacaactccc
   109081 tggccgttca gatttttacc tccataagca agaggtgcct ttaacatatt cgcccgggaa
   109141 gcaaacatgc tgttataatt caactcctgg tctgctgttg gaacagtctg tacatattcg
   109201 acaaatggaa gtgaggctaa ttcatttaac cgcattgcag ctatacgcaa ggtgatcacc
   109261 ctgtacgtct tataatcaac attgagaata tcaaaattct ttatctgtag ctcccggctt
   109321 actgtttcaa aagaaaatga tttgggaaag cttatccata catctaatgt ccctgcaatt
   109381 ctcactgcat gggccgggaa caatcctttt gctaacgctg gaggcatttt ttgttcagca
   109441 gtaagctcca ctatagccct tgctttcatc cggctgagta aatccgggtc caactgaccg
   109501 gtgatagtca ctgtgtatgc attataaggg atatattcta acaattcaat tcccgaatgg
   109561 agtaattgtt gtttttcata ttcggttggg attttttcaa attgtaccag cgcaaatgcc
   109621 ttaccggttg ttcttgcagc aatcctgttg aacgactcaa tcttatccgc agtaatattt
   109681 tttgaaggaa taaatgatcc actctttagc aagagattat agcgggaatc atcttgcgct
   109741 gccaatgaaa aagtgaatag cagcattgca gcaatcatgg gaattggata gaatctcata
   109801 gtgaatttta gtaaaactaa aagtaataca aatacctgag aaaagccaat gctgtgaagg
   109861 ccttaaagca ggtacggaaa taagctaagc ggggattgca gatgttgaaa aacaataatt
   109921 gtgcatgctt agtaagaacc ggtgcatttc ctccatacta ttcatattgt tggctatcaa
   109981 taaaaacaac ttacccgtct gtttaagttt gcccttatta gtttctgtct gcaagaactg
   110041 gatcactttc tggaaagtac ccgattcaaa ataaggtgag tcaggacggt tgataaagaa
   110101 acagcgtaac gtattatctt tcagactcat tttttcaaag cccagatcaa cagccagttt
   110161 gcggcagcga acagtaatga ataaatcttc cacctgttgt ggcaaagcgc caaacctatc
   110221 ttccagttcc tgcctcatta ccaaaaggtc atcctctgtt tcgcagttgt ccagccgttg
   110281 ataaagcgat aatcgttcgg tgatactctc tacaaaatca tcaggaatta aaatttccag
   110341 gtctgtatca atagtacaat cctgtacaaa atcatcttgt ttcgcaattt cttctttgaa
   110401 cagttcttta aattcagtcc gcttcaattc acggatagct tcatccagta tcttctggta
   110461 catctcaaaa ccaatctccg ccatgaaacc actttgttca ccaccaagca tattcccggc
   110521 tcccctgata tccagatccc gcatggcgat ctggaaacca ctacccaatt cgctatgctg
   110581 ctccaatgtt tgtaacctct ttcgtgaatc attaggtaag gtactcatcg gcggtgctaa
   110641 taaataacag aatgcctttt tattactccg ccctaccctg cccctcaatt ggtgcagatc
   110701 gcttaatcca aattgatgag cattgttaac aataatggta tttacattgg gaatatctac
   110761 gccactttct acaatattgg tacaaaccag tacatcatat tttctgtcaa taaaatcgag
   110821 gatatgtttt tcaagttcat gaccttccat ttgtccatga gcatagccaa tacttaaatc
   110881 aggacacagt gcctggatga tggcggccat ttcagctaga ccagcaatcc tgttataaat
   110941 aaagaatacc tgcccgcccc tttctgtttc ataataaatt gcttcacgga taaagtcttc
   111001 attgtacacc tgcacttccg tttgaatagg ttgcctgttt ggtggcggtg tattgatgat
   111061 acttaaatcc cttgcgccca tcagggagaa ttgcagggtc ctggggatgg gtgtagctgt
   111121 taatgtcaga caatcaactg cagtacgaag cgttttgatt ttttctttat gccccacacc
   111181 aaatttttgc tcttcatcca ccacaagtaa gccaaggtcc ttaaacttaa cttccttacc
   111241 aagcaaccca tgtgtcccga taataatatc aatcttacct tcttctagtt tcttcagcgt
   111301 ttcctttttt tctttggcag atttgaaacg attgatatag tcaactgtaa ctgggaaatc
   111361 ctttaatcgt tcactgaatg tctgaaaatg ctgaaaagca agaatagtag taggcaccag
   111421 tacagcagcc tgttttccat ccacgcaggt tttaaaagct gcccggatag ctatttctgt
   111481 tttaccaaag cccacgtcac cacagaccag cctgtccatt ggcgcaggcg attccatatc
   111541 cttttttaca tctgcagatg ccttactctg gtcgggtgta tcttcataaa taaaagaagc
   111601 ttccagttct gtttgcatgt aattatcagg cgtatgctga aacccttgct gggcttttct
   111661 tttggcgtac aacttaatca ggtcaaaggc aatttcctta acctttgttt tcgttttctc
   111721 ctttaattta ttccaaacat cacttcccag tttattcact tttggcacac taccatcctt
   111781 accggtgtat ttcgcaatct tatgcaaact gttgatgttt acatataata tatccgtgtc
   111841 tttataaatc aaccgcaccg cttcctgtaa tcttccattt acttccagtt tttgcaaacc
   111901 actgaaaata ccaacaccat ggtctatatg cgtaacaaaa tcccctggtt gcaattcacg
   111961 gagtgtacgg agcgttaaag ctttattttt attataggct tgtttgacct tgtatttgtg
   112021 ataacgttga aatacctggt gatcagtata acagactatt ttttggtctt catcaataaa
   112081 tccttcatga atagaaacat ttaccgggtt aaaaactata tcagctttca ggtcgtcgaa
   112141 aatactttgc aatctttcca gttgtttagg gttctctgca aaaatgtaaa cctggaattt
   112201 ttttgcctcc caatttttca ggtcttttat cagtaaatca aactgccggt taaaggaggg
   112261 ttgttcttta gtggaaaagt gaatagtaaa ggaggaaaaa ggaacgggaa aagaatccga
   112321 atgaccaaag cccaccgtat gtttctgaaa aagcttctct tcaaactctt ttacagtaat
   112381 aaaatcattt tcaccggtat ttttcttaac cagtttgtca tcctgctctt cgagtacttt
   112441 gtccctggcc ccctggacct ttagtccttc ctgcagccgc aggaaggcaa gcatatcttc
   112501 ttcacaatct gtcagtcgtt ccttacacaa atcataatcc tgcaaccaca caacagtatt
   112561 ctccggtaaa aaatcaagca aagaaatcct ttcctcactt gcaaactgtg tgtctacatt
   112621 cggaataata cttacctgta ataattttcg ttcactcaac tgagtttccg ggtcaacaat
   112681 acggatggaa tccacttcat tgccaaataa ttctacacgg tacggttttt cattgccaaa
   112741 agaataaata tccagtatgc cgcccctgat agcaaactgg cccggttcat atacaaagtc
   112801 tgttctttca aaaccataat ccgacagttt tagcaataag agctccacgt tcagcgtatc
   112861 ccctgactta atatgtataa tattggaaga aagtgtaccc ggcaatacta ctctttcaaa
   112921 aagtgcctcc gggtaagtta ccagtacttt tttattaccg cctgcggcaa cttttgttaa
   112981 cgcttctgtc cgcaacatca catgtgatga gttcagcagt tggtaatttt tcctgttctt
   113041 aaaagaagaa ggaaaataaa aaatatccaa agccccggtc aggttttcca gtgtattatg
   113101 gaaataggct gcttcttccc cgtcatttaa tataacaagg tgatttaccc gggataagga
   113161 aggatgcaga aaagcagcag caaccacaaa ctgggatgaa cttccctgta aattttcaag
   113221 aaatattttt tgaggctggg taaaagagag tttgtccgcc agttgaaaaa ggcggggtgt
   113281 attttgatac tgttccagca aatccattac actcatgaga ggtgcaaaga taacttatcc
   113341 cgtaaaaagc ggaataattt tcccaattca taacttataa ccctgttatt ggttttacct
   113401 ttatcaaaat caccaaataa tggcccttca accctggcgt aaaggcattg taacgaaaat
   113461 tgacgaccac actcataata cgagacggtt ctggatacag gttccagaaa tggaaaaatt
   113521 tgattttata cctggtcagt ttgtaacgct ggatctgcct atacatgaaa agcctaataa
   113581 aagatggcgt agttactcca tcgcttcgtg gcctgatggc accaatattt ttgaattact
   113641 gattgtacat gcagagggcg gactgggaac aacctatctt tttagtgaaa tctcggttgg
   113701 cagtgaactc accttccgtg gtgcccaggg agtttttaca ttgcctgaaa aaatcgaaaa
   113761 agatattttt cttatctgca ccggcacagg tattgctcct ttcagaagta tggtgaatta
   113821 tattcacctg cataacattc cctttaaaaa gattcatatg atctttggct gtcgtaataa
   113881 agcagacctc ctttattatg acgaactgac tgaattagaa aataaattac ctggctttca
   113941 ctatcatccc accttatccc gtgaacattg ggaggggcac aatggatacg ttcatcttat
   114001 atatcaggat ttatgcaggg ataaacagga tgcctctttt ttcctgtgcg gttggaagca
   114061 tatgatagac gaagcaaaga agatcatcac tgaaatggga tatgacagaa aagccattca
   114121 ccaggaatta tatggttaat tttacctggt tgacgaatta attttaagat gatattccgc
   114181 aacctcaacg ttgataactg tcatatttga ttttaacgca tagttcctat attcgtgcaa
   114241 atcaatctgc gtatgggtcc ttcctcgcta attgtactca ttattgctgc cattgccttt
   114301 tctggcattg caatccttat tgcaaagttt gtattaaaag cttctaccaa taaacaaaaa
   114361 ggcgaacctt atgaatgtgg catacccacg cagggtaaaa catggataca attaaatgta
   114421 gggtactacc tttttgcgct gatattcctg atttttgatg tggaacttgt ttttttatac
   114481 ccctgggcgg tggtggcaaa gagtataggt tgggtggcat ttatcgaaat aataattttc
   114541 ttttttattc tatttatggg atttttatat gcccataaga aaggtgccct taaatggata
   114601 taatattgta cggatgacag cacagaaaaa tacaacaaaa gactttcccg gacagataca
   114661 tgaaaccccg ggaggtggca ttgttctgag taaactggat gatgtcatca actgggccag
   114721 gtcaaattca ctatggcccc tgacatttgc caccagttgt tgcgggattg aatatatgtc
   114781 agtcgccggt gccaaatatg atttttcccg ttttggtttt gaagtggccc gtgctacacc
   114841 acgacaagcc gatgtgatca ttattgcggg tactatcgtc aataaaatgg ccccggtttt
   114901 aaaaagattg tatgaccaga tggctgatcc taaatatgtg attgcgatgg gcgcctgtgc
   114961 catcagcggt ggtccttttt tctataatac ttattctgta gtaaaaggag ccgaccatgt
   115021 gataccggta gatgtttatg tagcaggctg tccacccagg ccagaagcac taatgcatgc
   115081 tttaataaca ttacaacaga aaattaaaag cggattaacc agagaacaga tcagaacagc
   115141 caaagattag ttatgcagaa cgaagaacta aaagccaaaa taaccgaact gctatccgca
   115201 gccacttttg aagaaggtgg cgaatggctt aatattaatg tagagccggc ggactggtta
   115261 ccactggctc aacaacttcg taatgatgct tcgttatact tcgattatct tttttgcctg
   115321 acttgtattg actggaagac acatctgacc atggtgtatc atctgtcttc aacaaaacac
   115381 cggcacaata ttgttatcaa atcaaaattg gacaggaata aacctgaaat tcaaacggtt
   115441 tcccaggttt ggaaaacagc tgattttcat gaaagagaag tatatgaaat gtttggtgtg
   115501 aatttcctga accatcctga cctgcgttta ttgatattgc cagatggatg ggaaggaaag
   115561 aatcccctga gaaaagattt cgaagacccg ataaatatga tcaaactctg atatgctgga
   115621 agaagtaggt acaacgactg aaggtgactt agtcataaac gtaggaccac aacaccctgc
   115681 cacacacggt gtattgcacc tcgtcattac gttgaatggc gagacgatca aaaaagttga
   115741 acctcacctg ggtttcatcc atcgatctat tgaaaaaatg tgtgagagcc tgacctaccg
   115801 ccagttcatt tatgttacca gccggatgga ttatctctct tcacatatta ataaccatgc
   115861 ctgtgcccta tgtgttgaaa aaggtttaca agtggaaatt cctccaaggg cacaagtcat
   115921 cagggtattg atggatgaac tgacaagaat tgcctctcac gaactatggt ggggagctat
   115981 ggcaatggac cttggtgctt tcactccttt cttccatgct tttcgtgaaa gagaaggcat
   116041 caatgatatt atggaagaga cctgtggtgc aagactaacc atgaattata tggtacccgg
   116101 gggtgtaatg caggatcttc atccaaattt tcaaaagaaa gtaaaagagt tcctgcaatt
   116161 atataaacgc aagatccatg aatatgatga actggt
//
LOCUS       DAORTI010000002        70942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_166_length_70942_cov_7.958201, whole genome shotgun sequence.
ACCESSION   DAORTI010000002 DAORTI010000000
VERSION     DAORTI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 70942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70942
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_70942_cov_7.958201"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLO70_00525"
     CDS             complement(<1..247)
                     /locus_tag="PLO70_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72963.1"
                     /translation="MRKTTLFAILILMATQIKAQQQSFKKSTAYFEVGGNGLGFSVGY
                     ERQLGKKTGLGLHTGISLSGNKPGLVAGAKYLTDLGNH"
     gene            complement(415..1743)
                     /locus_tag="PLO70_00530"
     CDS             complement(415..1743)
                     /locus_tag="PLO70_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQZ72964.1"
                     /translation="MKKFFFAIILIYAGLSKAQSSYTPTPENLAARKVLQDNKFGLFI
                     HWGLSSVLGQGEWVMQNRKIKAADYQQYLRVFNPIDFDAEKWVLAAKNAGMKYIVFIT
                     RHHDGFSNWNTQYSDWKITSTLYKKDPLKLLAEACRKHDMKLGLYYSTLDWYRTDYPY
                     ETGRTGQFSGRTAKSNYASYLQFMKNQLTELLTNYGPIMSIWFDGHWDQTNVEGHADR
                     TSRIDWKYDELYGLIHKLQPACMIGNNHHLDPIAGEDFQMFEKDLPGENKTGLSFQQP
                     SDALPLETCETMNDTWGYNITDHHYKSVKQVIHLLVGAAGRNTNLLLNVGPMPNGEIQ
                     QQFTDTLLATGKWLQQYGASVYNTRGNIMPPQPWGVATRNGQQIYIHVLQSPQTPFIL
                     LPGFTENITSVEVMNSGKKLKWSKVPEGVFIYPEYPAQETIDHVVVLKVK"
     gene            2000..>2395
                     /locus_tag="PLO70_00535"
     CDS             2000..>2395
                     /locus_tag="PLO70_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c class I"
                     /protein_id="HQZ72965.1"
                     /translation="MKKVIFSMAIAAFLLSCGGGDSEKKEATDATTEPAKTEPATTDL
                     SDNPDYQKGMAIVADPKNLCLTCHKIDEKLVGPAYRDVANKYESTDANISMLAEKVVK
                     GGTGVWGEVAMPANTVISLDDAKAAVKYVL"
     assembly_gap    2396..2495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2962..3300
                     /locus_tag="PLO70_00540"
     CDS             2962..3300
                     /locus_tag="PLO70_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72966.1"
                     /translation="MDYEEIEKGKVHSLGEIVEYVPDGVVNKTVLKKPTGKIDIVSYD
                     TGKGLTGKVYPFDTFVLLLEGSAEIIIDKKVNEVHAFQGIVIPAHSTFTIKAIERFKM
                     LSITVKSGYE"
     gene            3557..3808
                     /locus_tag="PLO70_00545"
     CDS             3557..3808
                     /locus_tag="PLO70_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72967.1"
                     /translation="MQDHYSLLHTIYEIVKNDPQPERYSCRPRELILRRMQEWSEIQQ
                     QLQLLEQEELVVTEQQDTMVIRITNAGLEKVKEQNSLVG"
     gene            3952..4635
                     /locus_tag="PLO70_00550"
     CDS             3952..4635
                     /locus_tag="PLO70_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQZ72968.1"
                     /translation="MVNYDKYGCAFFGLLFAILIPACRQMNTAALAKGQPRSSFEKLM
                     DGNDRFSHWHPEHPDESRQRVIEISKAQHPFAVVISCSDSRVSPELIFDQGLGDLFVI
                     RTAGNLIGGLEIGSVEYAVEHLGVSLVVVMGHQHCGAVQAFVEGGETHGHIKDIVDSI
                     QKEKEIVDIPLDDKNRLEDCIRANILHGIHQLTTQSAIIKEKLDAKKLELKACYYDLE
                     KGLISLVNE"
     gene            complement(4649..5527)
                     /locus_tag="PLO70_00555"
     CDS             complement(4649..5527)
                     /locus_tag="PLO70_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQZ72969.1"
                     /translation="MLKTKLHKAGFIITSFMLMAAGSGNKYIPVPGDSAVINKTISRD
                     SIFTGEHIFDAEGLCVCLPEMSANELSEAPRIQLNEQAADFVQNHLKENGFYYDKVKA
                     KNASKFTLIEEIFQQYNIPVELKYLAVVESKLDNRIVSSAGAAGLWQFMPGAAKSFGL
                     KVSGKNDERKHNYKSTVAAAKCLIYLHNMFDDWLLTLAAYNSGPARVLSAIKKSGSRD
                     FWVLQNYLPVETRKHVKKFISVHYHFEGHGSLATLTKAETKAHTDAVAAFIAKQKEPV
                     IKDSLATSAETVKAAK"
     gene            complement(5916..6320)
                     /locus_tag="PLO70_00560"
     CDS             complement(5916..6320)
                     /locus_tag="PLO70_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72970.1"
                     /translation="MKKIFMNNRIIAIAFMTVFSAALTPAMANGTNPVLPVEIKYVGK
                     IQHNPVFQLSVAGNGTQDDFTINIKDEYGNSLYMENIKGDSFTKKFMLALEELGDYSI
                     QIQVTSRKTKSTVSYAVNSNTRNVQEVIVNQL"
     gene            6945..7535
                     /locus_tag="PLO70_00565"
     CDS             6945..7535
                     /locus_tag="PLO70_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQZ72971.1"
                     /translation="MAFKKDDVIIIEPREIFVGKIDAPVTLMEFGEYESEECAKVNEI
                     VKQLLEEYEGKIRFQFRHFPLTLIHQRSLKASESAVAAGQEGKFWEMHNVLFANRRNL
                     GTTSLKLHSKEAGIRNKKFLDDLVNGVYGWQVQDDLKEGINRGVKELPAFFINEVPFT
                     GKPTFANLSAAIELALKKGKSKVTVKKTAVKQKQRA"
     gene            complement(7618..7995)
                     /locus_tag="PLO70_00570"
     CDS             complement(7618..7995)
                     /locus_tag="PLO70_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72972.1"
                     /translation="MFSQAWNIYLPIIKILLKRSAGADQTLDMNRTDFERAAGGRKAK
                     LTFSISLYKGRLQHNSIAPPPVARELATILQEDEVTRRLIREYDFEFSMNTSFQLTIK
                     NITAPAEITEDAGNNEENSDTPA"
     gene            complement(8058..8309)
                     /locus_tag="PLO70_00575"
     CDS             complement(8058..8309)
                     /locus_tag="PLO70_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HQZ72973.1"
                     /translation="MTNDAILKYVETKGTNGKPLNIHFKQRSTISGLFIQGNDYDELK
                     SKNFWRIVSSTNIEQWKKTKDMNLARIFNGMEFTRLSAE"
     gene            complement(8392..8688)
                     /locus_tag="PLO70_00580"
     CDS             complement(8392..8688)
                     /locus_tag="PLO70_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQZ72974.1"
                     /translation="MNIYVSNLSFAVVDEDLRSYFAEYGEVTSAKVIMDKYTNRSKGF
                     GFVEMSDDAVAQKAIAELDGATVDGRAIKVSVAKPREERPARAARPSYSNNNRW"
     gene            complement(8917..9366)
                     /locus_tag="PLO70_00585"
     CDS             complement(8917..9366)
                     /locus_tag="PLO70_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HQZ72975.1"
                     /translation="MKTYGLPFFERFASKVTKATGKPFAFILACFIILGWIITGPVFH
                     FSDTWQLVINTGTTIITFLMVFVIQHAQNKDSVALQLKLNELIAATATASNRLVDVED
                     LSAEELETLKKFYVKLSALAKLEQDIHSTHSIDEAESKHLSKNQEQK"
     gene            9441..9947
                     /locus_tag="PLO70_00590"
     CDS             9441..9947
                     /locus_tag="PLO70_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQZ72976.1"
                     /translation="MVQFTATIHKFGKKGEKTGWTYFEMPADIVQQLKPGNKKEFKVK
                     GKLDGYAIRRVSVLPMGGGLFIMPLNTVMRRAIGKKQGAILTVQLTEDKSDFVFNKDF
                     MDCLDDEPAAKEFFQSLTGSHQRYFSKWIDSARTEPTKTKRIAMAITALARKRGYPEM
                     IRASQGKL"
     gene            complement(9948..11120)
                     /locus_tag="PLO70_00595"
     CDS             complement(9948..11120)
                     /locus_tag="PLO70_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQZ72977.1"
                     /translation="MFANRPKMLVITGLSALFIFMACKKDNADANDTAPVDPVAAALN
                     LPTTPFNYANIVQPAYLSGPNIIGQINTPANNPITDNGATLGRVLFYDKNLSLNNTIS
                     CASCHKQANAFSDPVAKSAGFNGGLTGRNSMTLINAKYYPNGSFFWDQRAATLEDQTL
                     IPIQDLVEMGMTLPQLEAKLRNLAYYPPLFSKAFGDNTITSNRIALAISQFIRSIVSF
                     ESKYDAGRSTYPPVPAPPPNATFPNFTAQENRGKEIFLSPQNTCAACHGSETFTAPQE
                     KNNGLDMATVDRGFGAVANNTALDATFKVTTLRNVELTAPYMHDGRFATLEQVVEHYN
                     SGVRNHPNLSPQLRLPNGQPRQLNLTTQDKAALVAFLKTLTDTNLTTDMKYSNPFK"
     gene            11257..12633
                     /locus_tag="PLO70_00600"
     CDS             11257..12633
                     /locus_tag="PLO70_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQZ72978.1"
                     /translation="MISSINEFRNPFFIGVAGTGMSALAQYLKGIGKNVSGSDRFFTP
                     GTYNEMQEKLETEGIRCFVQNGEGITQETDLVVVSTAIEDTVVEVQKAKQLNIPVIRR
                     SELLALIAQSKKTIAVGGTSGKSTTSAMLFDILEYAGLQPSIISGAGLISIIKNGKIG
                     NAKVGAGDWLVIEADESDGSIVQYKPEIGLLLNVDKDHQEIDELMSLFGIFKTNSKKF
                     VVNQSHTLSKQLSQDERNDFSTDSKFPAGYVAADFKQNGLEISFKINGVEFSLHLVGR
                     HNMENAVAASAVANQLGVDLVTCAAALQHYEGIYRRHQVLGNKHGVWLIDDYAHNPVK
                     CAAAIAACQPVAPKVIAWFQPHGYKPTNFLREDFVTEIASVLRPEDEIWMSEIFYAGG
                     TAVKDISANDLISDLKVLGKAAFFIENRNDFLSAVRSHLTDDCVLLLMAARDPSLEQF
                     AKQTWQEL"
     gene            complement(12636..13127)
                     /locus_tag="PLO70_00605"
     CDS             complement(12636..13127)
                     /locus_tag="PLO70_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72979.1"
                     /translation="MRWETIASAPDNEVYHLYKDDKKILTLTLHPFSNSARVEGETEK
                     RVFLIRKEGFLRNKTVLRNEYGIKIGELGHEHMQNFIDLNNERFFYTIHNNPLAELVI
                     YKESKDKPIVVCGLNTDKGSTGIHFKKDHSLTATSHPGLLMALCWYMFLPVTKENVPA
                     FAV"
     gene            13322..13801
                     /locus_tag="PLO70_00610"
     CDS             13322..13801
                     /locus_tag="PLO70_00610"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQZ72980.1"
                     /translation="MYDEKDNKFLERAIEISRQGMKSGQGGPFGCVIVLGDEIIGEGC
                     NQVTSSNDPTAHAEVVAIRQACNKLGTYQLTDCDIYTSCEPCPMCLGAIYWARPKRVI
                     YANTREEAAAIEFDDDFIYGEIIAKMEDRKIPFLHLPHPMAKEVFEAWKNWEGKIKY"
     gene            complement(13867..18135)
                     /locus_tag="PLO70_00615"
     CDS             complement(13867..18135)
                     /locus_tag="PLO70_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQZ72981.1"
                     /translation="MNHLYTVSTKEALLNTSIFPIARGHFLLTLLKSFCKLLGLPALL
                     LLLTALLSLPANSQTGLQFNGTNRYVTSGVTPANSILNTPVFTVETWFMRTGTGTTVS
                     TGTGGLPAVIPLVTKGTSEAETGPADINYFLGINTTGNVLCADFEEAQTSASPGLNHP
                     ISGITAIVNNVWYHAAATYNGTRWQLFLNGNLEAELTVGRTANAAVISPFAMASSIRS
                     NGTTIQGYFNGVLDEVRLWNYARTQVEIQASMNAEVNSGTGLLGRWGLNEGAGTSAVN
                     SIAGSPAGTLINTPVWVCGAPALDMTITAPSGLTATASSAFQINLGWTDNSGNETNFE
                     IERSTTGIGGTYSLLATVNANTNSYSDVTATALNEYCYRVRAKNCTVNSGYTGSTCAT
                     TPAEGNYALDLGSAGAYITFGQAMGLSTQNFTIETWFKRTGTGTANTTGTGGINIIPL
                     IAKGSQEADGSNVDANYILGIQSATNVIAADFEEGTGSTSTGLNHPITGTTIITDNVW
                     HHAAATFENGVFKIYLDGVLEASVNLSSSVFPQGASIQHAALGTMINSTGTTFGKFQG
                     LLDEARIWNYERTEAQLRSTINTQITTSQSGLLARWGLNEGSGTTVNGSAGTAFTGTI
                     TGTGWNWITPGSPFNITFNTPTTLSLQDGVNSYIGTRDTYTYDVSPGTVRGSETTFIQ
                     DKNATDDRVSLLRFDLSSIPAGSTIQSATLQFYVDVEGQGFNMYRMLVPWDEATVTFT
                     SIGNRHFAANNTDAESAINTNWPGVDGYTGLITVSVPASTIQNWIDGTLTNNGWLMIA
                     THADDGQQLRSREYGTAGDRPKLTVTYVNSSTNLPPNLPTNPSPANNAASPSTSPTLC
                     TTVSDADNTNLEVRFYGRKKTSSGAKFTIVGLPDTQFYTEEVQGTNSSGGGHNGIFKS
                     QTQWIADHRIDSSIAFVVQLGDCVQNGDNPPGADDQIEWKRADTSMKKIEFPNVPVTD
                     GIPYGICVGNHDQGTIGNPDLPSNNYNSFFGSSRFTGRAYYGGHYGTDNDNHYELFTA
                     GGIDFIHISIEYYPNGTTASLQPVLDWADALLKTYPNRKGILSSHNLLTTGNPANFQG
                     PGQKIYTDLKDNPNLILMLAGHVAGEGRRTDVFNGNTIHTIMADYQSGYSNGGNGYLR
                     IMQFLPAQNLLNIRTYSPYSNTSLTGSGSQFSLPVNLSEAFTLIGTNSNVAAGSETCI
                     QWPSLEQFTEYEWYVEVTDGVNTTTGPVWTFTTPANSPLPVNIISFKASAENNDKVKL
                     TWTTTYERDNSHFEIQRSTNGMYYTSIGTVPGRNNSNSLQEYTFYDNLPIKGISFYRL
                     KQVDNDARLTYSKVERVNITDPKTRVEVFPNPVSGNNFTIKLINDINGTVDVRVFDMK
                     GRLHLQQQHSNSNFITINHHLAPGLYTIKVTTNEMTEVKKIIVH"
     gene            complement(18384..18749)
                     /locus_tag="PLO70_00620"
     CDS             complement(18384..18749)
                     /locus_tag="PLO70_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72982.1"
                     /translation="MSENYKDILSNLSTDVDQETLLLYLQGKLSAEKKHEVEKQLLQS
                     DFEGDAAEGLQEFKDKEQLQYMVEMLNRDLKKRTEKKKKRRDKMKIPDQPWLYISILI
                     LIILIVISYLIIYKYLQNK"
     gene            complement(18753..19334)
                     /locus_tag="PLO70_00625"
     CDS             complement(18753..19334)
                     /locus_tag="PLO70_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ72983.1"
                     /translation="MKPDKYQHITDQELLEQFYADHNNEWLGILLQRYTLLLLGVSMK
                     YLKNEEEAKDSVQQIFLKVIQELQKYKVEYFKSWLYTVAKNHCLMKLREKQGKITTEI
                     NDRLATKPGEETDRQALLENDQTLELMDLSLKELNPEQQQCVTLFYLQKKSYQEVSEA
                     TGFSMLQVKSYIQNGKRNLKLLIEKKLQEKAKR"
     gene            19433..20200
                     /locus_tag="PLO70_00630"
     CDS             19433..20200
                     /locus_tag="PLO70_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQZ72984.1"
                     /translation="MDYAVVAVPAAPVRRKPRHNKEMVNQLLFGETVKVLKARGDLWV
                     KVRSLHDGYEGWMTHTLLEEIDEKMASQQSAFITADVMSIISIGDKKLHVPAGSSLPF
                     FENGIGKLGKLEYYFNGNAYNRNTQQPGDALVNELTSAWLNAPYLWGGRTPLGVDCSG
                     FTQVIFKLSGIDLPRDAWQQAQEGKVVKKLSEAQTGDLAFFDDKEEIVHVGILLDNQK
                     IIHASGKVRIDTIEKKGLINTDTGKRIYRLKAIRRYW"
     gene            complement(20174..20794)
                     /locus_tag="PLO70_00635"
     CDS             complement(20174..20794)
                     /locus_tag="PLO70_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72985.1"
                     /translation="MKKFYLLLFVLSISTAHAQKVYNIAQTYYRINPFNKEFSSFLIR
                     LMDDPLLTNKTINKKTDSTLFFLEGTYTSHSPFFFKTTRTKIILAEREHITDSTGQVN
                     NIFLYQLVGYAPPGKEGVNDIQKEFDKFCNRYKKGPYGVNIRELKLQDKKTGEIRDYL
                     LSSLIFSPLTIAWSTSKNNEENIFAITVRFTVVENRAYLPIPADGL"
     gene            complement(20858..21274)
                     /locus_tag="PLO70_00640"
     CDS             complement(20858..21274)
                     /locus_tag="PLO70_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQZ72986.1"
                     /translation="MLTQQIITALLIGLSAGVLSGLVGVGGGIIMVPALVFFMQYTQH
                     QAQGTSLAVLTLPVVILASIYYYHQCQKLGTPIDLKVVGLLAAGFVIGGFFGGKIALS
                     INQDVLKKIFAIILFYTALKMLNWDTMLVKWLRGIF"
     gene            complement(21302..21844)
                     /locus_tag="PLO70_00645"
     CDS             complement(21302..21844)
                     /locus_tag="PLO70_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQZ72987.1"
                     /translation="MLNVLHPWHGATYGEKAPATVNALIEIPQGSRSKYEVDKATGLL
                     KLDRVIYSSFQYPINYGFIPQTLGLDNDPLDILVICSQSIQSLCLVEANVIGNMQMID
                     SGQEDDKIIAVASKDPSVNHITRMEELPQHFLLELRNFFEQYKVLENKKVEIDTFQDQ
                     LIAYKIIKDSIHLYNETFKK"
     gene            complement(21855..22226)
                     /gene="crcB"
                     /locus_tag="PLO70_00650"
     CDS             complement(21855..22226)
                     /gene="crcB"
                     /locus_tag="PLO70_00650"
                     /inference="COORDINATES: protein motif:HMM:NF010791.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQZ72988.1"
                     /translation="MIKNILLVGLGGGLGSIARYLCQRWFAEVYSHSFPWGTFAVNIT
                     GCFLIGVFWGMSFRSFDSNEHWKLFLMTGLCGGFTTFSAFTLEGIGLLKENKTGLFFS
                     YVAASVLIGLVATYAGMKLTR"
     gene            complement(22223..22675)
                     /locus_tag="PLO70_00655"
     CDS             complement(22223..22675)
                     /locus_tag="PLO70_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HQZ72989.1"
                     /translation="MNIRIIILFLGLVFVGYSCDIKDKSAQSENNIDAARNFIRSALD
                     GKFNDARSYMLQDSLNNNYLDVAERSYKNADQAVKDGYKGSTIIIHTVNDVNDSTSFV
                     IYSNSFKNDHDTLRVIKSDNQWVVDLKYLYEHDADTLLNKVITNDTVK"
     gene            complement(22757..23368)
                     /locus_tag="PLO70_00660"
     CDS             complement(22757..23368)
                     /locus_tag="PLO70_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HQZ72990.1"
                     /translation="MKKVMNSIFAVAVTALIITGCKGKDGVADNPKAVLVAFFERMAK
                     KDLEGAAKLATKDSKSTMDMMKKAMDMAEKMKGTEKTEEDPAEDFKNMVVGDAKIDGS
                     NATVSVTNKKKNETVDFPLKKEDGSWKVDFSMGTLMKMGMDKAKEDGTEINPEDIDKM
                     KDFNMDSLKKGFETIDSALKSMDPKQMDQMKDAMKELEKMKQN"
     gene            complement(23420..24058)
                     /locus_tag="PLO70_00665"
     CDS             complement(23420..24058)
                     /locus_tag="PLO70_00665"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HQZ72991.1"
                     /translation="MKRTEFVFLFLLFLCSSCKNSQDTQLTPAMLAANFQQIDRAKSL
                     IKSGDIIFRNGIDDVSRAARSMNRKDTSYSHCGIVFIENDSVFVYHALGGSYNPSQKL
                     LREPIDSFCNPKDNDAFGIYRYGLTSGQLRLMQQTVLNYYQQGLKFDMYFNFRSDDKM
                     YCSEFVFKSLNKAVHGTYTDYVRLDTIPFGVTTDDIFRHPDCKLVKKESFKL"
     gene            complement(24086..25102)
                     /locus_tag="PLO70_00670"
     CDS             complement(24086..25102)
                     /locus_tag="PLO70_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQZ72992.1"
                     /translation="MTDTIINLETVNPIEFFGVNNGKLDILKKKFPLLKILSRGTQLK
                     LSGAPEQIETAREKISLIVSYLERNGHMSQNYFEQVLGGDDAEVIDHFKEKNPNEVLV
                     FGPNGKSVRARTANQKRLVQESDKNDIVFAIGPAGTGKTYTAVALAVRALKNKQVKKI
                     ILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMTTRTIEIAPL
                     AYMRGRTLDNAYIILDEAQNATELQLKMFLTRIGANAKAIITGDMTQVDLPRNQRSGL
                     QMATRILRNIDGIAHIELDEEDVVRHRLVKAIIKAYNKEHEKENEEYANRQTPAILKK
                     DKPQ"
     gene            25367..25849
                     /locus_tag="PLO70_00675"
     CDS             25367..25849
                     /locus_tag="PLO70_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQZ72993.1"
                     /translation="MAQEIKPIKRSSQLAPLSREHHDGLLFAWKIKQGLDNKTPLDKL
                     RKYSLWYWQQHIKPHFFQEEKILMPYMPANHPMAKQLKEEHDQIRELILSLDAEADER
                     DFTILADLLNKHIRFEERQLFAFLEEILTPEQLDKVFQQLEEHPLTCGEWNDEFWVKK
                     "
     gene            26098..28548
                     /locus_tag="PLO70_00680"
     CDS             26098..28548
                     /locus_tag="PLO70_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQZ72994.1"
                     /translation="MKFKLSLLLLFLLLAIFSQGQLYRVSGVVMDSKKEPVPLASVEI
                     KELRKGSVTKDNGSFEFFLERGKYDLVVSMIGFSTKVVTIYINNEDIVENVVLDPDES
                     NVLSEVVLKVKTRDRAEELIRNVIKQKETVLGAIGSYSCNIYIKAFQLDSGFTKKKKA
                     IEADTIIKEDYEGMSLTEVSLRLDRSAGGQIKEERVGVKKMGNTESLFYLSTTEGDFY
                     MFNNMIQAPPVSKIPFVSPLSYSGLMAYRFKTLKIDRTIKPKVYTIAIRPRQLSNATI
                     EGEITIQDSTWEVFSTVFRLPQAHMPEYDYMEVKQQYGKVGDSARMITRQQFSYYTKV
                     KGGRRYGETTAIYSDFELQKNFKRGHFGNEVSSTSQEAYEKDSVFWKSVRTEPLSKQQ
                     ALFARYQDSVYRYMRSETYLDSMDRVLNRITWPKMLIFGQVFNDHKKERMWILPPITS
                     LIQPVAFGGARLKLAGAYRKTYSSRKNLFLEADISYGFRNKDINGTVSIQRMYNPFNR
                     GQFKISAGRNFEFIYPGDAWVNVLKRSNIYLNNSLELSHELELFNGVMLLNKLEVALR
                     RSVSDYKVGNNADSIFGIPNEPPVEFDPYNATYNEIKLYYTPNMKYIREPKEKIYLGS
                     KWPTFYVTWRKGVPKLFNSKIDFDYLEFGMEQKINLGVAGNTSYIIKSGDFTNTNDLR
                     IIDYKFMRQGDPLFFQNPQYAFQALDSTFPVFDRYYQGNIVHEFNGALINKIPFFKKL
                     KLQEVAGGGFLITKDRSLRYFEFFTGIERVFKWPFNPLARVKLGAYVVGSVANKFNNP
                     VQFKIGLTTWDRFRNRWR"
     gene            complement(28563..29096)
                     /locus_tag="PLO70_00685"
     CDS             complement(28563..29096)
                     /locus_tag="PLO70_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72995.1"
                     /translation="MRTISVAILFVSMITCTTSIIAQVNKRNTWNLSVGADVIYPENY
                     FRKTHSWGYGSTIKAEYLFDKHTSLIFSTGAYSLSGKTSLLNPDARTVWGIPIKAGLR
                     YYLGNFYFGGDAGYLNQSGFEPNNGFVYSFFIGDEIITNKKNNNSLDLSIRHEAWVTD
                     RTRAFASIRLAYEFRIR"
     gene            complement(29191..31710)
                     /locus_tag="PLO70_00690"
     CDS             complement(29191..31710)
                     /locus_tag="PLO70_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ72996.1"
                     /translation="MKISLRQILLTATCLLLSILSFSQKKAAYVSGKVVDENENPLPK
                     VSVVVLGQSKGITTNDSGYFRIKVAANKAFALIFTYTGRRAEQKNFLLSEEEEETITI
                     RLEKSENTLQEVIITDQRDRREVGLIRPNPKTILNLPSAVTGVESLIKIFVGSNNELT
                     SQYSVRGGSYDENLIYVNDFEVFRPYLVRSGQQEGLSFINPEMVRNISFYNGGFQAKY
                     GDKMSSVLDIQYKKPKRFGGSAYVGILEQGLQLEGTSKNSKFSYLLGVRNRSNRNLLS
                     RQDTKGNYVPSSADFQALLNYQFSPKWQAELLSNISQTKFTLIPEFSQLTTSVFSPLY
                     SATIGVDIYFEGREKDRYQTNMLGFSLINQPSKKLKLKWMASRFENDEAENVDIIGTY
                     LFGDRDFDKRNPTYGLIVNPLGAGVYQNWARNELNIENWNVSHKGNYDKGKHAFQWGI
                     GYDKTTIADKLNEWEFQDSAGYALPYQPNMLQLSKVIKSNANLAINKFSGYLQDNILL
                     GDSSNSVTLTGGVRFNYNSLNQEFLITPRISASWKPRSKKDIIFRAAAGVYHQPPFYR
                     ELRRYNGTVNTNLQSQKSWQTVAGFDYNFIGIGGRPMRWTTEAYYKSMTDVVPYDVDN
                     VRIRYFGENNAKAYATGIEMRLHGELVPDAESWISLGIMKTAEDISGDHYYRYKNAAG
                     EFINAQSPDQVVVDSTRFDVGWLRRPTDRRITFGMYFQDYLPTNKNFKMFLNLLYGSN
                     LPYNIPGAVRYRNAAVIEPYIRADIGFSALLVDDDRSKRRSHSPFRNFDNIWASLEVF
                     NIIDRANVISYLFVKDFSNTVYLMPNRLTPRLVNFKIVARW"
     gene            complement(31759..32226)
                     /locus_tag="PLO70_00695"
     CDS             complement(31759..32226)
                     /locus_tag="PLO70_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ72997.1"
                     /translation="MFKHLLASALLILVTILFYNCSRTPCMNASLSFGLISFSDQESD
                     TIILRRYEKGSNFSTLKDSFLLDMGFTRTNDTLDLAYTTSTGLITSQYDYELYFPGAI
                     KLYRLSAIEEEAGEIRHAFWNNVKVGCVNKITSFTINNLVSYPPRYNYFYLSK"
     gene            complement(32251..32898)
                     /gene="rpe"
                     /locus_tag="PLO70_00700"
     CDS             complement(32251..32898)
                     /gene="rpe"
                     /locus_tag="PLO70_00700"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQZ72998.1"
                     /translation="MPIIAPSLLAADFLKLDQECTMLNESEADWYHLDVMDGRFVPNI
                     SFGPMIIEFIRKATTKVCDVHLMIEEPEKYAEQFKNAGADILTVHVEACRHLHRNIQQ
                     IKALGMKAGVAVNPHTPVSGLSDILHDIDLVCLMSVNPGFGGQKFIPYTLDKIKQLRK
                     MIDDSGLKVHIEIDGGVTLENAPSIIAAGADVLVAGNTVFKSTDPKATIRQLKQL"
     gene            complement(32928..33935)
                     /locus_tag="PLO70_00705"
     CDS             complement(32928..33935)
                     /locus_tag="PLO70_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ72999.1"
                     /translation="MNKVAKIMAPVLWMLILISACNDSDTTVTAYDEILNRPPYADLT
                     DSIKQEPGKAEFYFRRAILLNTNSLPEPALADFQKAWSLKKEEQYALGVATLLLDKKP
                     EEAITFLNTAIKDIPNSLLLQLSLARGYDAANKTEEALKIGDMILEKNPEQVDVLKMK
                     ADLLAKKGNTNEATAILEKAYTLTPFDVELNYILALKYAEAKNSRVLALCDSLIRADS
                     SGVHAEPYYYKGIYYSNINDKTKALSLFDEAVKHDYYFLDGYIEKGSLLYEMKRYPDA
                     LKVFNLALTISPKFADNYYWIAKCQQAMGQREEAKLNYQRAYGLDNTFIAAKDSADKL
                     K"
     gene            34056..35165
                     /locus_tag="PLO70_00710"
     CDS             34056..35165
                     /locus_tag="PLO70_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73000.1"
                     /translation="MKKLVLSLLIITGISFANSSCKNSSKIASTAIQVADTLRYPEES
                     HLKNIQQLTFGGDNAEAYWSYDGKYLVFQKTNPKEGINCDQIFVGKVPTKAGEKFTYK
                     MVSTGKGRTTCAYFTKDGKHIIYASTHLGSDDCPPVPDRSKYGNKYIWPLYSSYDIFM
                     ADLDGKIVKQLTNSKQYDAEATLSPDGKKMIYTSTKDGDIELYIMDLKTGAEKRITNT
                     LGYDGGAWFSPDGKKLIWRASRPKTEAEIKEYKELLAENMVAPTNMEVWVANADGSNA
                     RQVTSYGQANWAPTYMPDSKRIIFASNHEYKRGFPFNLYTINEDGTNLQKVSRDKGFD
                     AFPMFSPNGKKIVFSSNRNNGGNRDTNVFIADWVE"
     gene            35216..35653
                     /locus_tag="PLO70_00715"
     CDS             35216..35653
                     /locus_tag="PLO70_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73001.1"
                     /translation="METGMLHLHNVLRWVILILLLVTLYQAFAKKESIRSSSLWLMIA
                     AHTMLLIGLYQVIAGRFGILKGLPESVPSLMKDSFYRFYWVEHPILMVVAIVLITLAR
                     GKVKALNYKTAGWLLLIALIFILVAIPWPFREVGVNRAWFPGM"
     gene            complement(35875..36369)
                     /locus_tag="PLO70_00720"
     CDS             complement(35875..36369)
                     /locus_tag="PLO70_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQZ73002.1"
                     /translation="MTRIGLIADTHSFLDEAIFKNFAACDEIWHAGDFGTIELANQLA
                     SFKPLKAVYGNIDGNDIRTIYPEQLVFNCEDVKVMMRHIGGYPPRYNPETKKELLIHQ
                     PQLFISGHSHILKVIYDDKINCLHMNPGAAGKQGWHKVRTLIRFVIDGNDMKGCEVIE
                     LGKK"
     gene            36568..37725
                     /locus_tag="PLO70_00725"
     CDS             36568..37725
                     /locus_tag="PLO70_00725"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQZ73003.1"
                     /translation="MPHYYTLGTIPHKRHTQFRKPDGGLYSEQLFSTEGFSDDYALLY
                     HCHPPTQIIKTEPQVDVSPQVAEEKMLKHRSLEGFKVKPVKDYLQSRVPVLVNSDCHI
                     VLAAPQESMTDYYYKNTDADEMIFVHEGSGIVHTQYGELPFSYGDYIVLPRGTIYQVS
                     FNTPDNRLFIVESFSPLRYPKRYMSKYGQLMEHSPYCERDIRPPQDLKTFDEKGDFLI
                     KAKKKGMLYGLHYGTHPFDVIGWDGCCYPYIFSIHDFEPITGRVHMPPPIHQTFETSS
                     FVVCSFVPRLYDYHPDAIPAPYNHSNIDSDEVLYYVAGEFMSRKHVTKGMITLHPAGI
                     PHGPHPGAVEKSIGAKETKELAVMVDTFRPLMLTKQALEIENQDYVMSWAE"
     gene            complement(37785..38444)
                     /locus_tag="PLO70_00730"
     CDS             complement(37785..38444)
                     /locus_tag="PLO70_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73004.1"
                     /translation="MPFLNENSLVKIAVADDHHLFRESLCALIDTWENCKVVIQAANG
                     RQLLEKLHPKNLPDLVLVDLEMPEMNGYETLKAIKEKYPEIRLLVISLHYSEELVWRI
                     IKFGAQGFVNKNDDIARLKKAIAEMIRNGYFFSDHTASKMVKKAMQTGTLTIQNDLSE
                     TDVRFLKLVCSEKTYKEIAREMKLTGRQIEYLRNNLFDRFEVCTRTGLAVIALNKGLA
                     V"
     gene            38902..40215
                     /locus_tag="PLO70_00735"
     CDS             38902..40215
                     /locus_tag="PLO70_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73005.1"
                     /translation="MKKILGVLIINLIFFCGALKSQVTAEAFPMNPQSGSHNYFGVRV
                     TLAQTYTEDITVTGYIYDMGGGANTNNPFSLTVTAGNLTAETAANFYQTDPTATAEAT
                     LGTIATVYAGVTITYEADGCILKFNSLADADAVLNHLEADDDNYNDDYDSQYPNATED
                     ELDDLDEQNNFDNMKVYRDFEALFGGFCSKRAELESIENTWLSNNFNGTDPDDVDLTF
                     DDAENTIFNSSYSFKIGTDVYQLTSTGMYKNGILQDEGGNSINRKPDNFIELPEYGVL
                     SGPSSFPNYNGSFPNFSTANALVYDFFTVCKSNKKDKIFQEYDNGNKRVKATVSITST
                     VVRNGVHGKIKNYKSKNGNWKRSRAQLAVYCFGTYYTNLCADSHQFTDRKPSSGTKKR
                     AKISTRRHSPGHIPVQGTVWKAFTGEVAMYGEVGGSVRGQLTLTF"
     gene            40225..41067
                     /locus_tag="PLO70_00740"
     CDS             40225..41067
                     /locus_tag="PLO70_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73006.1"
                     /translation="MTIQTTLGKQGGIIRIILVCLFLQFTAQGYTQETNYEIKDHLSQ
                     KNNIKLQIGYGVIPNGVIEVKEGIYDLYTKPHSIYSVDLIYQVNLNPSWSVNTGIGFM
                     IIKSNFFEKIPINDLIGTGISRQEDSPPIIYYKGAYPRFSLPILLQKRSKIINRSFFE
                     FNGGFNINYNGFSKDERIIMNVADSNNQSINIFNANYKSNNNHKPWFGLLLGCGKGFF
                     LRDLNVVTVSLSAEVNTTKFLSADYEIIIPNKPISTGRYSVKGPLLQLSLGYVFRGTH
                     KNND"
     gene            41168..41989
                     /locus_tag="PLO70_00745"
     CDS             41168..41989
                     /locus_tag="PLO70_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73007.1"
                     /translation="MKIFLPILFWLWPVFALTQAKDSLQKLQSKPMLSKPVTNITKDY
                     KPEIFTSGFIDIVNNGQVNASARFIRLYIGEPGKFAIPVSFYGGVSNNTFQNNSPGTV
                     FSKSNSHLVNQYINPLSGLINISIEGVQFFNKKEKLTKAGLLYQVGERVLSGVRSGEI
                     SNPLTGKPVNFLNSFAATGIYFQTGAWERNNTKNVGVFWLAFRYHTCKSNVKQIKEFL
                     PAIETNGWYHGWSGGFGIEINKLVNIKAIYYKYSKAPEIEYGLPIYQFSFQYSMK"
     gene            42149..42766
                     /locus_tag="PLO70_00750"
     CDS             42149..42766
                     /locus_tag="PLO70_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQZ73008.1"
                     /translation="MVKKQKDQTTEQVILAAAKKVFVQKGMAGARMQDIADEAGINKA
                     LLHYYFRNKEKLFEVIFLEAAEKLFPKINAIFEADRPLFEKIENFCDEYISIVMDNPY
                     LPLFVLNEINQDPEYFLKKVWAGKAKPNPVKFLEQMEKEVKKGTIKKINPMHLLMNLL
                     SMTIFPFIAKPMFQKNLGMGEAQFRIAMEERKKEIPRFIIDSIKK"
     gene            42814..44076
                     /locus_tag="PLO70_00755"
     CDS             42814..44076
                     /locus_tag="PLO70_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ73009.1"
                     /translation="MKKHFLLAVLLLPAGLFAQSLQKITLTEAYELSQKNYPAVKQKD
                     LVKQTAAISLENLQKGFLPQFSLSGQATYQSDVTKVPFSLPGLTIESPSKDQYKLVAD
                     VSQMIYDGGMTKEQKALQQLNATVEDQKVEVELYKLKERINQLYLSILYLDEQLKQVD
                     LVKTDIQIGIKRVEAQVQNGVAFKSNLNMLKAELLKTEQRAIEVKSSKKGLLDALSLF
                     IGRELAAQVQLEKPVSAGIVANEIARPELKLYSEQQKFIGQQEKLITAKNLPRASLFA
                     QGGYGRPGLNMLLNEFDFFYIGGLRLNWSLGGLYTKKKEKEQVTINKKMVEVQKETFL
                     LNTSSQLKQQQAEIDKLQQLLVSDNEIISLRKSVTEAAKAQLENGVITANDFLKEVNA
                     EDQARQTLITHQVQLLQAQINYQTILGK"
     gene            44087..45031
                     /locus_tag="PLO70_00760"
     CDS             44087..45031
                     /locus_tag="PLO70_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQZ73010.1"
                     /translation="MQHIFKLAPIVLILFLSACKSGNGNFDASGTFEADEVIVSSELS
                     GKIISFSAEEGQSIPKDSIVAVVDAANISLQKEQVEANIQALSEKTADVTPQVRLLEN
                     QLAVQQSLLNNLLHEQTRIQNLLKQDAATGKQLDDIMAQVDVAKKSLALTQQQINVQR
                     SNVATQNRGILSEGKPLEKRVAQLDDQLKRATILNPVSGTVITKYAEQGEITAAGKAL
                     YKIADLSTMTLRAYITGTQISQVKLGQGVKVFVDDGPDKYKELPGMISWIADKAEFTP
                     KTIQTKDERANLVYAIKVKVKNDGYLKIGMYGEVKFSQ"
     gene            45065..45424
                     /locus_tag="PLO70_00765"
     CDS             45065..45424
                     /locus_tag="PLO70_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ73011.1"
                     /translation="MKNFKELLIWKKGIEVVKLVYKITKIVPAQEKFGIISQMTRAAV
                     SIAANIAEGSSRNSDKDYARFLQIALGSAFELQTYLVIVKEMNWADVNEVAQVELMLE
                     EEIKMIHSFIKRLSAKS"
     gene            45449..46363
                     /locus_tag="PLO70_00770"
     CDS             45449..46363
                     /locus_tag="PLO70_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ73012.1"
                     /translation="MSAVIVNNIIKRYGSKKAVITALQGVSFDVKQGELFGIIGPDGA
                     GKTSLFRILTTLLLADEGTATVDGFDVVKDYKSIRQRVGYMPGKFSLYQDLTVKENLD
                     FFATIFNTTVEENYALIKDIYSQIEPFKERKAGKLSGGMKQKLALSCALIHRPSVLFL
                     DEPTTGVDAVSRKEFWEMLTRLKEQGITIIVSTPYMDEASLCDRVALIQSGKILSINT
                     PQGIVDSFGKKLLAVKSNDMLHLLKDLKQCKEVEDAYPFGEYHHVVLKKDGEELLKSY
                     LEQKGHLFKLKEAKPNIEDCFMALMKTS"
     gene            46379..47122
                     /locus_tag="PLO70_00775"
     CDS             46379..47122
                     /locus_tag="PLO70_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ73013.1"
                     /translation="MNEKVIIVENLTKKFGDFTAVDHISFDVHKGEIFGFLGANGAGK
                     TTAMRMLSGLSLPTSGHARVAGFDVYKENEKIKQNIGYMSQKFSLYEDLTVKENIRFY
                     AGIYGMSNRFIREKTAMVLEKLHLAAEGDKLVKSLPLGWKQKLAFSVAIFHEPQIVFL
                     DEPTGGVDPVTRREFWQMIYEAAESGITVFVTTHYMDEAEYCNRVSIMVDGRIDAMDT
                     PQLLIETYDAISMDEVFLKLARKATRAAD"
     gene            47125..48231
                     /locus_tag="PLO70_00780"
     CDS             47125..48231
                     /locus_tag="PLO70_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73014.1"
                     /translation="MKQFIAFVKKEFHHIFRDKRTMFILLGMPVVQIIIFGFALTNEV
                     KNSRVAILDPSKDAATTSLIAQIEASRYFEVDQYIHSTNEIETIFRKGKIKLAIIFPS
                     HFREDLQHFNKAQVQLIADASDPNVANTLTSYASAIIMDYQNRITNDRKLPYTINTEM
                     RMLYNPELKGAYNFVPGVMAMVLLLVCTMMTAITIVKEKEMGTMEIMLVSPMKPLKVV
                     LAKAVPYLLLSSVNIASILLLSVFVLEVPINGSLTLLVAESLLFTLVSLALGLLISTK
                     VDSQQTALFISLVGLLLPTVMLSGFMFPIENMPLPLQVVSNAVPAKWYYTIVRSVMIK
                     GTGFQAVWKETLILAGMLFFFLGLAIKNFKIRLA"
     gene            48228..49346
                     /locus_tag="PLO70_00785"
     CDS             48228..49346
                     /locus_tag="PLO70_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73015.1"
                     /translation="MRTLKFLLQKEFRQIFRDPSILRLIFIMPVIQLIMLPFAADYEI
                     KNIKLAVIDHDHSSYSRSLVTKITSSGYFILTDYTGSFNQAFEKIEQDKADLILEIPA
                     SFEKDLVKESESKIFMAVNAINGVKAGLGSSYLRSIIQEYNKEVRTEWIQFPRFNPET
                     NIEVVYSNWFNSSMNYKVFMVPGILVLLLTMVGANLTAINIVKEKEIGTIEQINVTPV
                     RKFHFILGKLIPFWVLGLLIFSIGLGIARLFYGIIPAGNILTIYLFAAVYLFAVLGLG
                     LLISTYADNQQQAMLISFFLMMIFILLGGLYTSIDSMPGWAQAITKANPVSYFIEVIR
                     MVVLKGSSLADIKYQLLTILGFAIVLNGWAVVNYHKRS"
     gene            49408..49815
                     /locus_tag="PLO70_00790"
     CDS             49408..49815
                     /locus_tag="PLO70_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73016.1"
                     /translation="MLKITHLKEGDIITVDDDGIMREGTVVRVSREEHQALVDNGVQE
                     FWYSQEDMYSLPLDEGKLLEFGFTREEQDGAVKYKKDSFRLVTPQKGDFTNLEMWWRE
                     DRRHFDFPITVHELQNLYLDMTKVHLDKASNAV"
     gene            49925..50143
                     /gene="infA"
                     /locus_tag="PLO70_00795"
     CDS             49925..50143
                     /gene="infA"
                     /locus_tag="PLO70_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQZ73017.1"
                     /translation="MSKQPLIKQDGIILEALSNAMFRVKLENGHEILATISGKMRMHY
                     IRILPGDKVGVELSPYDLTRGRINFRYK"
     gene            50182..50298
                     /gene="ykgO"
                     /locus_tag="PLO70_00800"
     CDS             50182..50298
                     /gene="ykgO"
                     /locus_tag="PLO70_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HQZ73018.1"
                     /translation="MKVRASIKKRSVDCKIVRRKGKLYVINKKNPRFKQRQG"
     gene            50378..50758
                     /gene="rpsM"
                     /locus_tag="PLO70_00805"
     CDS             50378..50758
                     /gene="rpsM"
                     /locus_tag="PLO70_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQZ73019.1"
                     /translation="MARIAGVDLPKNKRGEIGLTYIYGIGPSTAKYILDKHNIDRNKK
                     VQEWNDDELTDIRNTITEELKVEGQLRSEVQTSIKRLLDIACYRGLRHRKGLPVRGQR
                     TKTNSRTRKGKRKTVAGKKKAAKK"
     gene            50915..51325
                     /gene="rpsK"
                     /locus_tag="PLO70_00810"
     CDS             50915..51325
                     /gene="rpsK"
                     /locus_tag="PLO70_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQZ73020.1"
                     /translation="MAKAQQQGGSAKAAAKKRVVKVDSYGDAHISATFNNIIISLTNK
                     TGQTIAWSSAGKMGFRGSKKNTPYAAQMAAADAAKTALDAGLKRVDVFVKGPGSGREG
                     AIRSLSQSGIEIVMIKDVTPLPHNGCRPPKKRRV"
     gene            51459..52064
                     /gene="rpsD"
                     /locus_tag="PLO70_00815"
     CDS             51459..52064
                     /gene="rpsD"
                     /locus_tag="PLO70_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQZ73021.1"
                     /translation="MARYTGPKTKISRIFGEPILGNGKWLGKNSNPPGQHGEKRKRKT
                     LGEYALQLREKQKAKYTYGVLEKQFRKTFEEAARRKGVTGENLIKLLEARLDNTVFRM
                     GIAPSRPAARQLVSHKHIEVNGEVVNVPSCQLKPGDVITIKDGSKDRTSITSVVRAKN
                     PKFSWLDWNETEFKGTFITYPERESVPENIKEQLIVELYSK"
     gene            52142..53143
                     /locus_tag="PLO70_00820"
     CDS             52142..53143
                     /locus_tag="PLO70_00820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQZ73022.1"
                     /translation="MAILNFQKPDKIILQKATDFEAQFEFRPLEPGYGVTIGNALRRV
                     LLNSLEGFAIIGVKIEGVEHEFATMKGISEDVVEIFLNLKQVRFKKTVDHEVTNEKIM
                     LSIKNRTEFTAGMIGEGTQTFQVMNPDLLICTLDSSARMDIELTIGKGRGYVPAEDNK
                     PKDAPLGYIPIDAIFTPIKNVKYTIENTRVEQRTDFEKLIMEVSTDGTIHPEEAVKQA
                     SRILIQHLMIITDENITFDNKEEKKEDLVDEQTLQLRKVLKTPLEDLDLSVRAFNCLK
                     AAKINSLSELVQYEQEDLMKFRNFGQKSLAEIEQVLTERGLHFGMDLVKLGIDLSEF"
     gene            53281..53772
                     /gene="rplQ"
                     /locus_tag="PLO70_00825"
     CDS             53281..53772
                     /gene="rplQ"
                     /locus_tag="PLO70_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQZ73023.1"
                     /translation="MRHGDKVKNLGRKKAHRDALLSNLACQLIQHKRIVTTTAKAKAL
                     RVYVEPLITKGKDNTTHQRRVVFSYLQDKEAVKELFDTVATKVAGRPGGYTRVIKLGA
                     RPGDNAETAMIELVDFNEIYGKGKGEAKTAAKKTRRSGGAKKKAEATDKVEAPAAEEK
                     AAE"
     gene            53927..56506
                     /locus_tag="PLO70_00830"
     CDS             53927..56506
                     /locus_tag="PLO70_00830"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQZ73024.1"
                     /translation="MDHQSNRALQQFRNEIGIKFQLYNSLFTSLPFHRIEKTGILLSL
                     LSNNCEEGYKKRSSPSDIIEDFFNKHTTYVKEDERTDLLFRFVQYIERQVVLFDALED
                     AAFKSVNDLNGVGTLKHLESEVLQGNKQDELAVKLKDFAIRLVLTAHPTQFYPGPVLG
                     IINDLAKALAENNTNLVNTYLQQLGKTPFFKKKKPTPYDEAVSLIWYLENVFYPAAGR
                     IISSLKNQFPDAVSKNNPVIRMGFWPGGDRDGNPFVTSDITLQVSNALRSSIVKCYYM
                     DVRRLRRRLTFAGVEVFLQELENKLYNNLFIPGFKTNLKKDEVLITLNQIKEVIIHQH
                     NGLFLHMVNNLINKIEVFGLHFASLDIRQDSSVHGKVLQDIAADQQLLPAGYAGLTEQ
                     EKIEILSSLTGIADADSLSDPLSQDTLKTIRVVKEIQQFNGTEGCSRYIISQCNSALN
                     AMEVYGLFLLAGWKKEEMAIDIVPLFETIDDLQEAATVMRTLYTHPVYKEHLKRRGNQ
                     QTIMLGFSDGTKDGGYLMANWSIYKAKEELTRISRENEVDVIFFDGRGGPPSRGGGKT
                     HKFYASMGHNISNKEIQLTIQGQTVSSNFGTIDAAQFNIEQLLNAGISNDLFSNRDIT
                     LNENEESLLQELAEAGFTAYKSLKDHPYLADYLLQVSPLRFYSETNIGSRPAKRGASS
                     GLSLKDLRAIPFAGSWSQLKQNVTGYYGVGSALQQMEKQGKLLQLQKLYHQSLFFRTL
                     LDNCEMAMMKSFFPLTAFLSNHKQFGELWHMIYDEYELTRQYLFKITGHNELMADYPV
                     EQLSIQMRERIVLPLVTIQQYALTKIRELEEQLAPAHTKETFEKLAMRCSFGIINAGR
                     NSA"
     gene            56560..57435
                     /locus_tag="PLO70_00835"
     CDS             56560..57435
                     /locus_tag="PLO70_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HQZ73025.1"
                     /translation="MNKVKVGLVQMSCTNKKDQNLNKAIEGVRKAAAGGAQIVCLQEL
                     FTSLYFCDVEDYENFKLAEPVPGPSTEALQKVAGELGVVIIASLFEKRAQGLYHNTTA
                     VIDADGSYLGKYRKMHIPDDPAYYEKFYFTPGDLGYKVWKTKFGTIGVLICWDQWYPE
                     AARITALMGAEILFYPTAIGWATAQDEATNTEQYGAWQTIQRSHAIANGVHVVSVNRV
                     GLEQNGAMKFWGGSFVSNPFGTILYKASHEEEEVKVLELDLAKTDQYRTHWPFMRDRR
                     IDSYQPITKRFIDEE"
     gene            57517..57870
                     /locus_tag="PLO70_00840"
     CDS             57517..57870
                     /locus_tag="PLO70_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ73026.1"
                     /translation="MAFKFEKLIVWQKAVDLSDVVNQVTKQFPKDEVYILTSQIKRAA
                     DSVSLNIAEGSTGQSSPEFNRFLGIALRSAIEVVGCIFLAKRREYISDDDFDKIYKMC
                     EEILVMINALRNSLK"
     gene            57883..58965
                     /locus_tag="PLO70_00845"
     CDS             57883..58965
                     /locus_tag="PLO70_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQZ73027.1"
                     /translation="MSTQKHSVFPSSGLSDFPKNSGYYFPAEFAPHVATWLSWPHKEA
                     SWPGKIHTIYPSYAKFIKELTKGELVRINVADEAMKSFAIGHLQKEEVDLSKVEFFFH
                     PTNDAWCRDHGPAFLINPDAKIKKVIVDWGYNAWGNKYPPFDLDDVVPTLIGKHFNIP
                     VYHPGIVMEGGSVEFNGMGTVMTSTACLLNPNRNPHLIQQEIEEYLCNYYGMEQVLWV
                     DEGIVGDDTDGHIDDTVRFVNEDTVITVIEEDKNDENYTLLQHNLRQLKEMRLINGKQ
                     LNIVELLMPEELIYEDQRLPCSYANFYIANKSVIVPTFRSARDEKALRIIQDCFPSRE
                     VVGIDSTDIIWGLGSFHCLSQQEPLV"
     gene            complement(59034..60143)
                     /locus_tag="PLO70_00850"
     CDS             complement(59034..60143)
                     /locus_tag="PLO70_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ73028.1"
                     /translation="MLQKLFVAAISMLSFTLSIAQTASINNNTADSTATVAAGERKEA
                     SLTITGSVDAYYKLDFANTKVNSLTSFTQTHNAFSLGMASVKFEHKGDKVGAVADLGF
                     GPRAKDFSYTDNGITQAIKQLYVSYSPADWLKFVAGTWGTHVGYELLDPQLNRNYSMS
                     YMFTNGPFSHTGLKAEVSMGKHGAMLGIANATDFRIPMDGQINKKFLLAQYSYAPTEN
                     IKIYLNYVGGQNPDTSKVNQFDGVITAKISDKFNIGYNGTINSTRFWDGAKNIESQSW
                     WGSALYLNYDPQPWFGLTLRGEYFGDKNHLKIMASPDGSNIFATTFSANLKSGGFTFI
                     PEFRLDNSNKEIFIDKDGVTKKTAANFLIAAIYSF"
     gene            complement(60242..61642)
                     /locus_tag="PLO70_00855"
     CDS             complement(60242..61642)
                     /locus_tag="PLO70_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQZ73029.1"
                     /translation="MSKRKAKQILPFVLLVAIAIIAMFVKPAADYVPGEADPQYSSSD
                     IAWVLVSTALVFLMTPGLAFFYGGMVNRKNVISTMIKSVVAAGIVGVLWVICGFSLAF
                     GESINGFIGNPSTYAFYNGVKSGAAWPLANTIPLSLFSLFQLMFAIITPGLVVGAVAE
                     RIRFTSYVLFIVLFALLVYAPVAHWSWHPEGFLAKMGAWDFAGGTVVHITAGCAALAG
                     ALVLKRRKSHIENKEIPPANVPYVLIGTGLLWFGWFGFNAGSAVGATPLAVNAFGTTT
                     TAASSAGLAWMFFDVIKGKKPSVLGFCIGAVVGLVAITPAAGFVGIPQSIIIGLVGAL
                     VSNIAVSIKQKSKLDDTLDVFPCHGLGGMVGMLLTGVFASTAVHGIKDGPQGLFYGNP
                     EFFFTQLKAMSIVVAYSFTVSFGIFKFINFVIPMRVSESEEEEGLDATQHDEKYLQGT
                     LLVHNNGSIEEKPVEH"
     gene            complement(61835..63343)
                     /locus_tag="PLO70_00860"
     CDS             complement(61835..63343)
                     /locus_tag="PLO70_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HQZ73030.1"
                     /translation="MGNPTGFKEFGRELPGKQPVQERVQHYNEFVNRYTDEQLNQQAA
                     RCMNCGVPFCHHGCPLGNVIPEFNDAVYKKDWKEAYAILSSTNNFPEFTGRICPAPCE
                     TACVLGINQPAITIEEIEKHIIEIAFEKGFVLPKKPNLRSGKKVAVIGSGPAGLAAAA
                     QLNYAGHSVTVFERDDAPGGLLRYGIPDFKLEKWVIDRRIKLMEEEGIIFKCLANVGV
                     NVSINDILREFNAIVLAGGSTAARNLFIPGREFNGVHFAMDFLKQNNKRVAGKDPLAN
                     AAIESNILSEELKATGKNVVVIGGGDTGSDCVGTSNRQGAKSVTQFELLPKPPEERNN
                     YMPWPTYPMLLKTTTSHEEGAKRQWAVATKEFIGDEKGNLKALKIIDLEWKIVDGRPA
                     QFVEVAGSEKEIPCELALLAMGFVHPQHVGFISDLGIGLDERGNVKASEKDYRTNISK
                     IFSAGDMRRGQSLVVWAISEGRECARKVDEFLNNNAPSMLESRDNILQFQAL"
     gene            complement(63389..67906)
                     /gene="gltB"
                     /locus_tag="PLO70_00865"
     CDS             complement(63389..67906)
                     /gene="gltB"
                     /locus_tag="PLO70_00865"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HQZ73031.1"
                     /translation="MAGNQGLYDPSFEHDACGIGFVANIKGHKSHQHISDALTVLENM
                     EHRGACGCEKNTGDGAGIMIQTPHEFFFDECLRLGVHLPSFGKYAAGMVFFPREIRLR
                     EECRDILNRTAEKLGLEILAYRKVPVNTADIGPTALSVEPEMEQVFVACPDHISNSEE
                     FERKLFVLRNYASHLINNTVRKDDIGFYMASLSYKTIVYKGQLTSHQVRQYFPDLSNK
                     RVVSAFGLVHSRFATNTFPSWKLAQPFRFIAHNGEINTLQGNLNWLKTSEKGFTTPYF
                     SKEEMEMLLPIVTEGQSDSACLDNIIELLTLTGRSLPHVMMMLIPEAWDGHEEMDPVK
                     KAFYEFHSSFMEPWDGPASISFTDGKIIGATLDRNGLRPSRYCVTTDDRVIMASETGV
                     LPVDPASIIEKGRLQPGKMFVVDMEKGKIISDTELKKEICSQKPYGEWLNKYKIRLEE
                     LPEPRIMFTHLEHDQVFKYQKAFGYSTEDLDTIIAPMAVEGKEPIGSMGTDVPLAILS
                     DQPQHLSSYFKQLFAQVTNPPIDPIRERLVMSLATFAGNNGNLLLEDPLTCHSVALKH
                     PVLSNYELEKIRSIDTGLFQSKTIQCYFKADGKPGSLQKGLDRICEYAVHAVEDGFKV
                     LILQDRAIDSTHAPIPSLLSTAAIHHHLIRKGMRGQVGIIVEAGDVWEVHHFACLVGF
                     GATAVNPYLALSSIRDMRESGKLQTTLTQDELKKNYIKAVNDGLLKVFSKMGISTLQS
                     YQGAQIFEILGLNKEVVNKYFTGATSRIEGMGLDEIAREALAKHFFAFNKKDIPVDRL
                     PAGGIYQWKRKGEFHLFNPQTIHLLQHATKMNDFNTFKKYSKLVNDQGEKACTLRSMF
                     QFRKNRPSVSIDEVEAAENIYKRFATGAMSFGSISWEAHTTLAIAMNRLGAKSNTGEG
                     GEDERRYEVMGNGDSMRSAIKQVASARFGVTSLYLTEADELQIKMAQGAKPGEGGQLP
                     GHKVDDWIGKVRHATPGVGLISPPPHHDIYSIEDLAQLIYDLKNANRKARISVKLVSK
                     AGVGTIAAGVTKAKADVVLISGHDGGTGASPVSSIKHAGLPWELGLAETQQTLVKNKL
                     RSRVVVQADGQMKTGRDIAVAALLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGVAT
                     QDPGLRKRFKGDPDHVVNFFKMITQELREIMAELGFRTVNEMIGQVDNLEMRENIEHW
                     KYKNLDLSSVLYKEPNSMYTTLFCSEEQDHGLADTLDWKLLQAAAPAIESTQKVSALF
                     NIKNTDRTVGTILSNEITKKYRAAGLPDNTIHFTFNGTAGQSFGAFNTNGITLELEGD
                     ANDYFGKGLSGAKLIIYPPKTATYSPEDNSIIGNVALYGATSGEAYIRGKAGERFGVR
                     NSGANVVVESVGDHGCEYMTGGRVVIIGDTGRNFAAGMSGGIAYVYDVKGKFSDNCNK
                     EMVDLDTCDLNDKNELFTMIQKHFEYTGSTVAKFILEDLDNQLQHFVKVFPRDYKKVL
                     LQKAEGIKQNA"
     gene            complement(68047..69303)
                     /gene="ilvA"
                     /locus_tag="PLO70_00870"
     CDS             complement(68047..69303)
                     /gene="ilvA"
                     /locus_tag="PLO70_00870"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HQZ73032.1"
                     /translation="MAQTINNTVQLDFLSAHLRLKKVVNRTPLTYNHSLSKKYQCHVF
                     LKREDLQVVRSYKLRGAYNMMSLLPVEQLQKGVVCASAGNHAQGFAYSCKKLNAKGVV
                     FMPIITPKQKVNQTKMFGENYIEIKLIGDTFDDCAVAAKQFTEENDMTFIPPFDDYRI
                     IEGQGTVAVEILEDQPDVDYLFVPVGGGGLSAGVGAYFKTYSPKTKVIGLEPEGAPSM
                     FEALKAGHPVTLDSIERFVDGAAVKRVGDLTFTICKDVLDGMHLVPEGKVCSTILKLY
                     NEDAIVVEPAGALSIAALDDYADKIKGKNIVCIVSGSNNDIDRMQEIKERSLQYEGLK
                     HYFLISFAQRPGALREFVNNVLGPTDDITRFEYMQKTNKENGPALVGVELQSRSDYEL
                     LLKKMQDSNIGFSEINKNDQLFGYLV"
     gene            complement(69313..70446)
                     /gene="ilvC"
                     /locus_tag="PLO70_00875"
     CDS             complement(69313..70446)
                     /gene="ilvC"
                     /locus_tag="PLO70_00875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQZ73033.1"
                     /translation="MAKLNFGGVEENVVTREEFPLSKAQQVLKNETVAVIGYGVQGPG
                     QALNQKDNGINVIVGQRKNSKTWDKAVRDGFVPGETLFEIEEALQRGTIICYLLSDAA
                     QIALWPTVQKHLTPGKALYFSHGFGITFNEQTGIIPPKDVDVFLVAPKGSGTSLRRMF
                     LQGRGLNSSYAIFQDATGKAFDRVIALGIAVGSGYLFETDFKKEVYSDLTGERGTLMG
                     AIQGIFAAQYQVLRERGHSPSEAFNETVEELTQSLMPLVAENGMDWMYANCSTTAQRG
                     ALDWWKKFRDATAPVFNELYDSVATGKESQRSIESNSKADYREKLEEELKELRESELW
                     QAGKTVRSLRPENQSKTVTTTAATASSTKEVFSKLAQKPESVG"
     gene            complement(70489..>70942)
                     /gene="ilvN"
                     /locus_tag="PLO70_00880"
     CDS             complement(70489..>70942)
                     /gene="ilvN"
                     /locus_tag="PLO70_00880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQZ73034.1"
                     /translation="HRFTIVINEIEEVVKKLCRQIEKQVEVLKAYYNTEEEIVWQELA
                     LYKVSTDAITEKVKVERLLREYGARAVAIRKDYTVFETTGHREETDNLIKALEPYGLI
                     EFVRSARVAIIKASNSFHDRLKEFEKKEPGEEVIENEYLDKNEEVFTM"
BASE COUNT        20770 a        13877 c        14958 g        21237 t
ORIGIN      
        1 tatgattgcc aaggtcagtt aaatatttgg cgcctgctac taagccgggc ttattacccg
       61 aaaggcttat cccggtatgc aaaccaagcc cggtcttttt tccaagctgt cgttcatagc
      121 caacagaaaa gccaagcccg ttaccaccta cttcaaaata ggctgtactt tttttaaatg
      181 attgctgctg ggcttttatt tgagtggcca ttaaaattaa aatggcaaac agggttgttt
      241 ttctcatgct agctggtttt aaaaggcagg gaagtatatc agaacgaatg gatcctgtta
      301 tttattatct gcaacaactg atcagggggt tctcctttgt cttagaattc aacaggaaaa
      361 ctaaaactaa caggagagtt gtgcatcgtt tgcaacgatt gaccggaggg aaacttattt
      421 tactttcagc actaccacat gatcaattgt ttcctgtgca gggtattctg ggtaaataaa
      481 aactccttcc ggcactttgc tccattttaa ttttttcccc gagttcatta cttccactga
      541 tgttatattt tccgtaaacc ccggtaacag gataaaagga gtttgcggtg actgcaacac
      601 atgaatgtaa atttgctggc cattgcgggt agctaccccc cagggttgcg ggggcatgat
      661 attaccacgg gtattataaa cagaagcgcc gtattgctgt agccatttac cagtggccag
      721 taaggtatcg gtaaactgtt gttgaatttc accattcggc atcggtccca catttaaaag
      781 caggttggta ttccgcccgg ctgctcccac cagtaaatgt atcacctgct taacggattt
      841 ataatgatgg tcggtgatgt tatagcccca ggtatcattc atcgtttcac aggtttccag
      901 tggcaacgca tcagaaggtt gctgaaaact cagacccgtt ttattttcac cgggaagatc
      961 tttttcaaac atctggaaat cttcaccggc tatcgggtcg agatgatgat tattcccaat
     1021 catacaggca ggttggagtt tgtgtataag cccgtacagt tcatcatact tccagtctat
     1081 gcgggaggtt ctgtctgcat ggccttcaac attggtttgg tcccaatgcc cgtcaaacca
     1141 tatactcatg atggggccgt agttcgtcaa taattctgtc aactggtttt tcataaactg
     1201 caggtaactg gcataattac tttttgcggt tcgtccgctg aattgaccgg tgcggcctgt
     1261 ttcataagga taatcagtcc tgtaccagtc aagcgttgag taatagagac ccagtttcat
     1321 atcatgtttc ctgcaagcct cggctaacag cttcaacgga tcttttttgt agagtgtgct
     1381 ggtgatcttc caatcactgt attgggtatt ccagttagaa aaaccatcgt ggtgccttgt
     1441 gataaagaca atgtatttca taccggcatt tttggccgcc agcacccatt tctccgcatc
     1501 aaaatcaatt ggattaaata cccgcaggta ttgttggtaa tctgctgctt tgatcttccg
     1561 gttttgcatc acccattcgc cctggccaag cacactgctc aagccccaat ggatgaacag
     1621 gccaaattta ttatcctgga gaactttccg ggctgccagg ttttcaggag tgggtgtata
     1681 agatgactgt gccttactaa ggccagcgta tatcaatata atggcgaaaa aaaatttctt
     1741 catcggaaac ttattgtgag tggaagttac tgaatttttg tagggttcat tgctggcatt
     1801 atggtagtta tctgctgtaa atagcatcct ctgcggagat aaacctgtat tagatataat
     1861 gccttacctt atcattatcc atgtcgggaa cttgaatggc atccggacta atgctgtcat
     1921 ggcatcacac tttggttgta tagaaacaag attgcaatac atgacctatc tttactcact
     1981 aaaccaaaaa taaaataata tgaaaaaagt gatcttctct atggctattg ctgcattttt
     2041 attatcctgt gggggtggcg acagtgaaaa aaaggaagca acagatgcaa caactgaacc
     2101 tgcaaaaaca gaacctgcaa caactgatct gagcgataac ccggattatc agaaagggat
     2161 ggcaattgta gcagatccaa agaatctctg tttaacctgc cataagatcg atgaaaaatt
     2221 ggtaggtccg gcgtacaggg atgttgccaa caagtatgaa agtactgatg ctaatatcag
     2281 catgctggca gagaaagtgg ttaaaggggg aacaggtgta tggggagaag tggccatgcc
     2341 tgctaatact gtcatcagtt tagatgatgc caaagctgca gttaaatatg tattgnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcta atactgtcat cagtttagat
     2521 gatgccaaag ctgcagttaa atatgtattg ttgctccgca ataaataaac tgttgaatag
     2581 ttttcgttaa cctgttatga ccaaagcttt caaaaagttg tcatctttca ggactaaaca
     2641 aattgatttc caacccgggt ctctaacagg attcgggttg ttttttactt atatagaccg
     2701 ggagatgttc tttaaccagg aaatctctcc aaccggcaat gaattggttt tgaccaatag
     2761 gttgtgatgt gccgtaatga tccgatgtag atcccaaatg cggaaatcaa agctgaagct
     2821 aaaaacagca tttatgtatg cctttgaaac aaaaggcagt tcaaaatgtg tgaattatgt
     2881 aacttctgaa cagaaatata tattgattgt tggtataaaa gacctttctt gcattattta
     2941 cagtagtata aaatttttag aatggattac gaagaaatcg aaaaggggaa agtacatagt
     3001 ctgggagaaa tagtagaata tgtgccagat ggagtagtca ataaaaccgt gctgaaaaag
     3061 ccaactggaa aaattgatat agtctcctac gatacgggga aagggttaac aggtaaagtt
     3121 tatcctttcg atacttttgt attgctgctt gaaggaagtg ctgaaataat cattgataaa
     3181 aaagtaaatg aagtccacgc ttttcaggga attgtcatac cagctcactc aacctttact
     3241 ataaaagcca ttgagcggtt caaaatgctt tcaatcaccg tcaaaagcgg gtacgagtag
     3301 gctttcccgg cattttcatt tcccggatta gaagcagggg attgtatata tcctttcttt
     3361 accctgcctt ttcctttgtt atatgcaata gtttatgttt acaaaattct tcagtttcaa
     3421 tcaggagatg ggtactactt tttctttaat tttaacactg ctaattattt tttactaaca
     3481 attacctatg tttttgttga attttttgtt gataaattaa tcacctgaga aataaatttt
     3541 ccgataattt taaaatatgc aagaccatta tagccttctc cataccatct acgaaatcgt
     3601 aaaaaatgat cctcagcccg aacgttactc ctgcaggcca agagagctca tattgcgcag
     3661 gatgcaggaa tggtcggaaa tccagcagca attacaatta ctggaacagg aagaattggt
     3721 ggtgaccgaa cagcaggata ccatggtgat ccgtattacc aatgccgggc ttgaaaaagt
     3781 aaaagagcaa aacagcctgg ttggataata ccaggaccac aggcattcca gtcattatat
     3841 acagactaca cactgattaa actcattttt ctggctgatg accctcaagt tgccgggcag
     3901 aaaacctgaa tagttttacc tgctttatta ttttgcagtt cagaaatcag tatggtcaat
     3961 tacgataaat atgggtgtgc attttttgga ttgctgtttg ctattcttat tccggcctgt
     4021 aggcaaatga atactgcggc cctagcaaaa ggacagcccc gctcatcgtt tgaaaaatta
     4081 atggacggaa atgaccgctt ttctcattgg catcccgaac accccgatga aagcaggcag
     4141 cgggtaattg aaatctcaaa agcacaacat ccatttgcag tagtgatttc ctgttcggat
     4201 tcaagggtat ctccggagct gattttcgac caggggcttg gcgatttatt tgtaataaga
     4261 acagcaggta acctgattgg cggattagag atcggaagtg ttgagtacgc agttgaacac
     4321 ctgggagtaa gcctggttgt tgtaatggga caccagcact gcggagcagt acaagcattt
     4381 gttgagggtg gagaaactca tgggcatatt aaagatatcg ttgatagcat acaaaaagaa
     4441 aaagagatcg ttgatattcc tttagatgac aaaaaccgcc tggaagattg tatcagggct
     4501 aatatactcc atggtattca ccaactgaca acccagtcag caataattaa agaaaagctt
     4561 gatgctaaaa agcttgaact aaaagcctgt tactatgacc tggaaaaagg acttattagt
     4621 ttagttaatg agtaaatttt tacaaccatt atttagcagc ttttacagtt tctgcactgg
     4681 ttgccaatga atcttttatt acaggttctt tttgtttggc tataaaagca gccaccgcat
     4741 ccgtatgggc ttttgtttct gcctttgtca gcgttgcaag gctgccgtgc ccctcaaaat
     4801 gataatggac actgataaat tttttcacat gctttctcgt ttcaacagga agatagtttt
     4861 gcagcaccca gaaatcacgg ctacctgatt ttttgatagc agacaaaacc ctggcaggac
     4921 cactattata agcagcaagt gtcaataacc agtcatcaaa catattgtgc aggtaaataa
     4981 ggcatttggc tgctgccacg gtacttttat aattatgttt acgttcatca ttttttcctg
     5041 acacttttaa tccaaaactt tttgctgcac cgggcataaa ctgccacaga ccggctgctc
     5101 ctgcagaaga tacaatcctg ttatccagtt ttgattccac tacggccagg tattttaatt
     5161 caaccgggat attatactgc tgaaatattt cttctatcaa tgtaaacttt gaagcgttct
     5221 ttgcttttac cttatcataa tagaatccat tttctttgag atgattttgt acaaaatctg
     5281 ctgcctgttc attcaattgt atccttggcg cctcgctaag ttcgtttgcg ctcatttcag
     5341 gcaggcatac acacaaacct tctgcatcaa atatatgttc gcctgtaaaa atggaatccc
     5401 tgctgatcgt cttatttata acagcagaat caccgggtac aggaatatat ttattgccgg
     5461 aaccagctgc catgagcatg aagcttgtaa taataaatcc agccttatgt aattttgttt
     5521 ttaacatctt acagatttta gttaacaatt gttgcaggta ttggattttg gaaaaatgaa
     5581 ttcagtcgta ttgctgaatt cgtgttaagg tgattctgtt gggtaagtct taccagtttt
     5641 tcatttacac agaaccgata gtaaccttga taatttggct gtgcaaaggt aacccgcaaa
     5701 tgccctatag tactgcattt gcagcttagt tttccactga caggggtaag tgtatgagca
     5761 tcagtaaaga attttgttaa aactgctaaa tcgtcaggtg tcaataggaa aactacgact
     5821 attggttaag cattcgataa caatagtata ctgataaata aaaactgcgg atcaattgat
     5881 ccgcagtttt tcaaattaat aaatccggta tgtgattata attgatttac tattacttcc
     5941 tgtacgttac gggtattact gtttaccgca tacgacactg tgcttttagt ttttctgctg
     6001 gtaacctgga tctgaatact gtaatcaccc aactcttcta aggccagcat aaatttttta
     6061 gtgaagctgt ctccttttat attttccata tacagagagt tgccatattc atctttgata
     6121 ttgatggtaa agtcatcctg tgtaccatta ccggcaacag atagctggaa taccggattg
     6181 tgctggattt tgcccacata cttaatttca acaggtagta cagggtttgt gccgttggcc
     6241 attgcgggtg taagagctgc agaaaataca gtcataaatg caatagcgat aatgcggttg
     6301 ttcataaaga tctttttcat aatgataatt tttaaattaa taaaacttgt taaatacaaa
     6361 cttgatgggg ttgcaatcgg ttcagtgaac aaaaatcggg ggcaaacaac agggcaagta
     6421 gaggttagta aatccggatt tttttctatg gaagcaatct gatattgctt tgatgatgta
     6481 aaagtaaggg cggtatattg actcatcaaa ctaacttgaa agggatttgt ggatagccaa
     6541 ggataaagag gggattataa gttatttgtt ttcagtattt tatctttatc tatggtatag
     6601 atatccaagc ttctaaagca ggctttttat taacttatta catccatttt attttcgttt
     6661 aaatgggttg gcaaactgtc agataataaa attgatgata agcccaattt catttcatct
     6721 tcagttatag cgtaatgcac tgtttttcag tgtggtgggg tgttgttagg aaattaatgg
     6781 taagcggatt tggcagggtg caaaaaaata ttatcttcaa ccgattcaga gaaatgactg
     6841 attggcgtaa ctgcctttcg ttcaaccaaa tggcgggaaa tttgtttatt tataatatcg
     6901 ttcaggctaa taaggcctca acttgaaaaa aggagaataa aataatggct tttaaaaaag
     6961 atgatgtaat tattattgaa ccaagggaaa tctttgttgg taaaatcgat gcccctgtaa
     7021 cattaatgga gtttggcgag tatgaaagcg aagaatgcgc taaagtaaat gagattgtaa
     7081 aacaattact ggaagaatat gaagggaaga taaggtttca gttccgtcat tttcccctga
     7141 cgcttattca ccaacgaagt cttaaagcca gtgaatctgc tgtagccgcg ggtcaggaag
     7201 ggaaattctg ggagatgcac aatgtgcttt ttgccaatcg tcgtaacctg ggaacaacca
     7261 gtttgaaatt acattccaaa gaagccggca tacgcaataa aaaattcctg gatgacctgg
     7321 taaatggagt ttatgggtgg caggtacagg atgatctgaa ggaaggaatt aaccgtggtg
     7381 taaaggagtt gcctgctttc tttataaacg aagtaccttt taccggaaag ccaacctttg
     7441 ctaacctgag tgctgcaatt gaactggcgc tgaaaaaagg taagtctaag gtaacagtaa
     7501 agaaaacagc cgtaaagcaa aagcagcggg cctgatcgcc tgattgccca gtataaaaat
     7561 taaatcctgc cggtagtact actggcagga tttattattt tgggacaccg cattttttta
     7621 tgcaggtgta tctgaatttt cttcgttatt tccggcatct tctgtaatct cagccggtgc
     7681 agtgatgttt tttatcgtta attgaaatga ggtattcata ctaaactcaa agtcatattc
     7741 acgaataagc cggcgggtta cttcatcctc ctgcagtata gtagccagtt ctcttgccac
     7801 cggtggtggt gcaatagaat tatgctgtaa gcggccttta taaagggaga tggagaatgt
     7861 gagttttgct ttacgtccgc ctgcggccct ttcaaagtcg gtacggttca tatccagtgt
     7921 ctggtctgca ccggcagaac gtttcagtaa tattttaatg atgggtaggt aaatattcca
     7981 tgcctgggag aacataagct gatattttaa atgaatgtat ggcctgttta gtcacactac
     8041 tatgacaggt aattatatta ttctgctgac aatctcgtaa actccattcc gttgaaaatc
     8101 cgggctaggt tcatgtcctt tgtttttttc cattgttcga tattggtact gcttacaatg
     8161 cgccagaaat tttttgactt cagttcatca taatcatttc cctggataaa aagacccgaa
     8221 atggtactgc gttgtttgaa atggatattc aacggtttgc cattagtacc ttttgtttct
     8281 acatatttta agatcgcatc atttgtcata ctaaatatat tttaaaaatt gtaaaaaagg
     8341 ggagctaaat ccagctcccc cttatagtat catgtgtaat gatgaccttg attaccaacg
     8401 gttgttgtta gaataagaag gtcttgcagc cctggctggt ctttcttctc ttggcttagc
     8461 cacacttact ttgatggcac ggccatctac agtagcacca tcgagttcag caattgcttt
     8521 ctgggcaaca gcgtcatcac tcatttccac aaaaccaaag cctttgctgc ggtttgtgta
     8581 tttgtccata ataacttttg cagaagtaac ttctccatac tcagcaaaat aacttcttaa
     8641 gtcttcatcc actacggcga aacttaaatt tgaaacataa atgttcatgt aaaaaaatta
     8701 acttttaaaa atacttgaga ataacgcaag ggtaaagaag actaactatt agcagatcct
     8761 tcgattaact caggattagg gcgtagcaga aaaaatcgtt cacttacaat ctgtcgtctg
     8821 actcaaatta acaatgcgaa ggtaagggaa aataatcaga accgggtttt tattttgatg
     8881 tattttaatt catcttaacc acaattcaat gtccattcat ttttgttctt ggttcttgct
     8941 caggtgtttt gattctgctt catctatgga atgtgtgctg tgaatatcct gctccagttt
     9001 ggctaatgca gaaagtttta catagaactt ttttaatgtt tccagttctt cagctgagag
     9061 atcttccaca tccaccagcc ggttactggc agtggcagta gcagctatca attcattcag
     9121 ctttaattga agcgccacac tgtctttatt ttgcgcatgc tggattacaa ataccatcaa
     9181 aaatgtgatg atagtggttc cggtattgat cactaattgc caggtgtcgg aaaaatgaaa
     9241 aacagggccg gtaataatcc agccaagtat aatgaaacag gccaggataa atgcaaaggg
     9301 tttacctgtt gctttggtga cttttgatgc aaatctttca aaaaagggga ggccatatgt
     9361 tttcatataa ctgagttttt cgggaatgat tattttagtg ctgccaatag ctgctgaaat
     9421 ttagccttta attcttatct atggtccaat ttactgctac tattcataag ttcgggaaga
     9481 agggggaaaa aacaggctgg acctattttg aaatgccagc tgatattgtt cagcaattaa
     9541 agccgggtaa taaaaaggaa tttaaggtaa aaggcaaact ggatgggtat gcgatcagga
     9601 gggtttcagt attacctatg ggaggtggcc tattcattat gcctctgaat acagtaatgc
     9661 gaagagcaat aggtaaaaag caaggagcaa tactaacagt gcagttaaca gaagataaaa
     9721 gtgattttgt ttttaataaa gattttatgg attgcctgga tgacgagccg gcagctaaag
     9781 aatttttcca atctcttacc ggatcgcatc agcgttactt tagcaaatgg atcgatagtg
     9841 ccagaacaga gcctactaaa acaaaaagga ttgccatggc cattactgca ttggccagaa
     9901 aaaggggcta tccggaaatg atcagggctt cacagggaaa gttgtaacta tttaaatgga
     9961 ttggaatact tcatatccgt tgtcagattt gtatcagtca atgtttttag gaaagccacc
    10021 agggctgctt tgtcttgtgt ggtaaggttc aattgcctgg gctgaccatt gggcaatctt
    10081 aattgtggtg atagattagg gtgatttctc acaccggaat tataatgctc taccacttgt
    10141 tccagggtgg caaaacgacc atcatgcata taaggtgcgg taagttctac atttctaaga
    10201 gtggttactt taaaagtggc atccagtgca gtgttattgg ctacggcacc aaagccacgg
    10261 tcaacggttg ccatatccaa tccgttattt ttttcctgcg gtgcagtaaa tgtttcggaa
    10321 ccatgacagg cagcacaggt attttgagga gaaagaaata tctctttgcc cctgttttcc
    10381 tgggcagtaa aattggggaa tgtcgcattt ggtggcggtg caggaacggg cggatatgtt
    10441 gacctgccgg catcatattt agattcaaaa gaaacaatgg atcgtataaa ttgagaaatg
    10501 gctaatgcaa ttctgttact ggttatagta ttatcgccaa aagctttgga aaaaagcggt
    10561 ggataatagg caaggtttct gagttttgct tctaattggg gtaaggtcat ccccatttcg
    10621 accaggtcct ggatcggtat taatgtctga tcttccaatg tagcggcacg ctgatcccag
    10681 aagaaactgc catttggata atattttgca tttatcaatg tcatagaatt tcttcctgtc
    10741 aatcctccgt taaaaccggc actttttgct acagggtcag aaaaagcatt ggcctgctta
    10801 tgacacgagg cacaggaaat ggtattattg agcgaaaggt ttttatcata aaacaatacc
    10861 ctgccgagag tcgctccatt gtctgtaata ggattatttg ccggggtatt tatctgtcca
    10921 atgatattcg gaccgctcag gtaagcaggt tgtacaatat tggcataatt aaaaggagta
    10981 gtgggcagat taagcgctgc tgcaacaggg tcaaccggtg ccgtgtcatt agcatcggca
    11041 ttgtctttct tacatgccat aaaaataaac aaggctgata atcctgtgat gacaagcatc
    11101 tttggtcggt tggcaaacat cagtggcttt ttggtggtgc tttgagaccc cggttacgaa
    11161 ttaaagttta aggtactatg aatttatttt ttacaacaaa tgatcaaggc acatttcctg
    11221 tacgcattac cctgatttat ttcttttatt tgcagtatga tatcctccat caatgaattc
    11281 cgcaatcctt tttttatagg tgtggccggt accggtatga gtgctttggc tcaatattta
    11341 aaaggtatag gcaaaaatgt atccggcagt gaccggttct ttacaccggg tacttacaat
    11401 gaaatgcagg agaaactgga aacagaaggc atacgttgtt ttgtacaaaa cggcgaaggc
    11461 atcacacagg aaacagatct ggttgtagtg tcaaccgcta ttgaagatac agtagtagaa
    11521 gtacagaagg caaagcagct caatatacct gtcatacgca gatctgaact tttggcattg
    11581 attgctcaaa gtaaaaagac aattgcggta ggaggcacca gtggcaagag tacgaccagc
    11641 gccatgttgt ttgatatatt ggaatatgcc ggcttgcagc ccagtattat cagcggcgcc
    11701 gggttgataa gtattattaa aaatggaaaa attgggaatg ctaaagtagg agccggggat
    11761 tggctggtga ttgaagcaga tgaaagtgac ggctctattg tacagtataa acctgagatc
    11821 ggcctgttat tgaatgtaga taaagatcac caggagattg atgaattgat gtccctgttc
    11881 ggtatattta aaactaacag taaaaagttt gtagtaaatc aatcccacac tctttcaaaa
    11941 caactttcgc aggatgaaag gaatgatttt tctactgata gtaaattccc ggctggatat
    12001 gttgctgcag atttcaagca gaatggatta gagatctcct ttaaaataaa tggagttgaa
    12061 tttagtcttc atctggtagg caggcataat atggaaaatg cggtggctgc ttcggcggtt
    12121 gctaaccagt tgggtgttga cctggttacc tgtgcggcag cattacaaca ttacgaaggt
    12181 atttaccgtc gtcaccaggt tttagggaat aagcatggtg tatggctgat cgatgattat
    12241 gcgcataacc ctgtaaaatg tgctgctgct attgctgctt gccagcccgt ggcacctaaa
    12301 gtaattgcct ggtttcagcc gcatggctac aaacccacta attttttgcg ggaggatttt
    12361 gtaactgaga ttgccagtgt actccggccg gaagatgaga tatggatgag tgaaatattt
    12421 tatgccggag gaacagcagt caaggatatt tctgccaatg atcttatcag tgatttaaag
    12481 gtgttgggga aagctgcctt tttcattgaa aacaggaatg attttctttc agcagtccgt
    12541 tctcatttaa cggatgattg tgtattgtta ttaatggcag caagagatcc atcactggaa
    12601 caatttgcta aacaaacctg gcaggagtta tagactcaga ctgcaaatgc aggtacattt
    12661 tcttttgtaa caggtaaaaa catataccag caaagtgcca tcagcaggcc ggggtgagag
    12721 gtggctgtaa gcgaatgatc ttttttaaaa tggattcctg tacttccttt atccgtatta
    12781 aggccgcaaa ccacaatcgg tttatctttt gattctttat aaataaccag ttcggccagt
    12841 ggattattat gtatggtgta aaagaaccgt tcattattca gatcgataaa attctgcatg
    12901 tgctcatgac caagttcccc gatttttatt ccatactcat tacgcaacac tgttttattg
    12961 cgaaggaatc cttctttacg gatgaggaaa actctttttt ctgtttctcc ttccacccgg
    13021 gcagaattgg aaaagggatg taatgtaagt gtcaggattt ttttatcatc cttatataaa
    13081 tgatacactt cgttatcggg cgctgaagca attgtttccc atctcatagc agaatgttga
    13141 ttaatgattc ttaagtaaga taagcagctt tctgaaaggg gagtcttagt taacctaaat
    13201 ttaatattaa cttcatattg gagccgtttg ggtgcataag ctgtggaaaa tccggtttta
    13261 attcacaatt ttgtattcag gtaaaaaggg gatgaataac cttattgtct aaaaatcaat
    13321 tatgtacgac gaaaaagata acaaattttt ggaacgggct atagaaatct ccaggcaggg
    13381 gatgaaaagc ggccagggcg gtccatttgg ctgtgtgatt gtgttgggtg atgaaataat
    13441 aggcgaaggc tgcaaccagg tcacttcttc taatgaccct accgcccatg ctgaagtagt
    13501 agccatacga caagcttgta ataaattagg cacttatcag ctaacggact gcgatattta
    13561 taccagttgt gaaccatgtc caatgtgtct tggtgctatc tattgggccc gccctaaacg
    13621 ggttatatat gccaatacaa gggaagaagc agcagcaata gaatttgatg atgactttat
    13681 ttacggggag atcattgcaa aaatggaaga cagaaaaatt ccttttctgc atctgccgca
    13741 ccccatggca aaagaagtat ttgaagcctg gaagaattgg gaaggaaaga taaagtactg
    13801 atacaggttt ggcatataat aaaagaagcc cggtataacc gggcttcgct gtatttgatt
    13861 ttccagttaa tgtactatta tttttttaac ttctgtcatt tcattcgttg taacttttat
    13921 tgtatatagc cccggggcta aatggtgatt gattgtaatg aaattactat tactatgttg
    13981 ttgctgtaaa tgtaaacgac ctttcatgtc aaatactctc acatctactg ttccatttat
    14041 atcatttatt agtttaatag taaagttgtt tcctgaaaca gggtttggaa aaacttcaac
    14101 cctggttttc ggatccgtaa tattgactct ttcaactttt gagtaagtta acctggcatc
    14161 attatctact tgttttaagc gatagaatga aatacctttt atgggaagat tgtcatagaa
    14221 agtgtactcc tgtaaactgt tggaattatt cctacccggt acagttccga tgctggtata
    14281 atacattcca ttggttgatc gctgaatttc aaaatgacta ttatcccttt catacgtagt
    14341 ggtccaggtg agcttaactt tatcattgtt ttcggcactt gctttaaagc tgattatgtt
    14401 tactggtaac gggctattgg ctggtgtcgt aaatgtccag acaggccctg ttgtcgtatt
    14461 cacaccatct gttacttcaa cataccattc atattcagtg aactgttcga gagatggcca
    14521 ttgtatgcag gtttccgaac cagcagcaac attagaattt gttccaatta aagtaaacgc
    14581 ttcagataga tttactggca gagagaattg actgcctgat cctgttaaag aagtattact
    14641 ataaggagaa taggtccgta tgttcagcag gttttgagca ggtaaaaact gcataatacg
    14701 gagataacca tttccgccgt tggaataacc actttgataa tctgccatta ttgtatggat
    14761 agtattacca ttgaatacat cagtacggcg tccttctccg gcaacatgtc ctgccagcat
    14821 taaaattaaa ttagggttgt cttttaagtc ggtatatatt ttttggccag gtccctggaa
    14881 attagcagga ttaccagttg tcaataagtt atgactggag agaatacctt tcctgttggg
    14941 atatgttttc aataatgcat cagcccagtc caaaaccggt tgtaatgatg ccgttgtgcc
    15001 attcggataa tattcaatgc tgatatgaat gaagtcaata ccgccggctg tgaataattc
    15061 gtaatgatta tcattgtctg ttccataatg accgccataa taagcccttc cagtgaaccg
    15121 ggaagaacca aagaaggagt tataattatt actgggtaaa tctggattgc caattgttcc
    15181 ctggtcatgg ttgccaacgc aaattccata aggaatacca tcggtaacag gaacattcgg
    15241 gaactcaatt tttttcattg acgtatcagc ccttttccat tctatctggt cgtctgcacc
    15301 cggtggatta tctccgtttt gcacacaatc gcctaattgt actacaaagg caatactgct
    15361 gtctatacgg tgatcggcta tccattgtgt ttgggactta aagattccat tatgtcctcc
    15421 accactacta ttagttcctt gtacttcttc tgtataaaat tgtgtatccg gcaaaccaac
    15481 tatggtgaat tttgcaccag aactggtttt cttcctgcca tagaatcgta cttccaggtt
    15541 ggtattatcc gcatcactaa cggtagtgca taacgttgga ctggtggatg gagaagctgc
    15601 attattcgcc ggggaaggat tagtaggcag attgggaggc aggttagtag aactgtttac
    15661 ataggtgact gttagtttag gccggtcgcc tgctgttcca tactctcttg accgtaattg
    15721 ctgcccatca tcggcatgag tggcaatcat tagccagcca ttattagtta atgtgccatc
    15781 tatccagttt tgaatggtgg atgcaggaac agatacggta attaatccgg tatatccgtc
    15841 aactccgggc cagtttgtat ttattgctga ttctgcatct gtattatttg ctgcaaaatg
    15901 cctgttgcca atagaggtaa aggtcactgt tgcttcgtcc cagggcacca gcatccgata
    15961 catgttaaag ccctgtcctt ctacatcaac ataaaattgc agtgtagctg attgaatggt
    16021 tgatcctgca gggattgatg ataagtcaaa acggagtagt gaaactctgt catccgttgc
    16081 atttttatct tgtatgaaag ttgtttcact acctctcact gttccggggc taacatcgta
    16141 agtataagta tcccttgttc cgatatagct attcactcca tcctgtaaac tgagtgttgt
    16201 tggagtattg aatgtaatat tgaatggtga acccggtgtt atccagttcc atcctgtacc
    16261 agtgatagtt cctgtgaaag ccgttcctgc acttccgttc acagtagttc cggatccttc
    16321 attaagaccc catctggcga ggagacctga ctgggaagta gttatttgtg tattaattgt
    16381 tgaccgcagt tgcgcttccg ttctctcata gttccagata cgggcttcat caagcaatcc
    16441 ctgaaactta ccaaaagttg ttccggttga gttgatcatt gttccgaggg ctgcatgctg
    16501 aatgcttgca ccttgcggga aaactgaaga actgagattc actgatgctt ccagtacacc
    16561 gtcaagatag attttgaata caccattttc aaaggttgct gcggcatgat gccagacatt
    16621 atctgtaatg attgttgtac ccgtaattgg atggtttaat ccagtactgg ttgatcctgt
    16681 tccttcttca aaatccgccg cgatgacatt ggtagccgat tgaatgccga gtatgtagtt
    16741 cgcatcaaca ttgctgccat ctgcttcctg tgaacctttg gcaataagtg ggatgatgtt
    16801 aattccgccg gttccggtag tgtttgctgt gccggttccg gtgcgtttga accatgtttc
    16861 tatagtaaag ttttgggttg agagacccat tgcctgtcca aatgtgatat acgcacctgc
    16921 ggatcctaag tcaagcgcat aatttccttc tgcaggtgtt gttgcacaag ttgaacctgt
    16981 atagcctgag ttaacagtac agttttttgc acgaaccctg taacaatatt catttaatgc
    17041 tgttgcggtt acatcactgt aagaattggt atttgcattt actgttgcca ataatgaata
    17101 ggtcccgccg ataccggttg tggagcgttc aatttcaaaa tttgtttcat tgcctgaatt
    17161 gtctgtccaa cccagattaa tctggaacgc agaagaagct gttgcagtga gaccagatgg
    17221 tgcagtaatc gtcatgtcaa gtgccggtgc accacatacc cataccggag tgttgatcaa
    17281 agtgcctgca gggcttcctg ctattgaatt aactgctgat gttccagcac cttcatttag
    17341 accccatctt cctaaaaggc ccgttccgga atttacctca gcattcatgc ttgcctgtat
    17401 ttctacttgt gtgcgggcat agttccagag acgtacttca tcaagaaccc cattaaaata
    17461 accttgaatg gtagttccgt tggaacgtat ggatgacgcc atagcaaaag gagaaataac
    17521 agcagcatta gcagttcgtc caacagtcaa ttctgcttca aggttcccgt taaggaaaag
    17581 ttgccacctg gttccgttat atgttgctgc cgcatgatac catacattat ttacaatcgc
    17641 tgttataccg gaaatcggat gatttaagcc gggactcgct gatgtctgtg cttcctcaaa
    17701 gtcagcacac aatacatttc cagtggtgtt gatgcccaga aaatagttaa tatctgccgg
    17761 ccccgtctca gcttctgagg tgccttttgt aacaagcgga attactgccg gcaaacctcc
    17821 ggtacctgtt gatacagttg ttcctgttcc agttctcata aaccaggtct ccactgtaaa
    17881 tacaggtgta ttaagaattg aattagctgg tgtaacacca gaagttacat acctgtttgt
    17941 gccattgaat tgcagcccgg tttggctgtt agccggtaat gaaagcaatg cggttaatag
    18001 gagtaacaat gccggtaatc ctaacagttt acagaatgac tttaataaag ttaataagaa
    18061 gtgccccctg gctattggga aaatactggt atttaataaa gcctctttag ttgaaacggt
    18121 atatagatgg ttcataaaaa aaagttatag tgataaattg ggcatgacag aataatagat
    18181 cattggctgt tcaggggtgt aattggaaca gattgatcag aagataaacg gaaaattaag
    18241 tgatggttat tctgtaagag ttggagaatt gaggaaagcc ggttgtaaaa gtaatattag
    18301 ttcttacaaa tgcaaatgat taagtaagta ttgaaatttt gaaacagtgg aaattgtctg
    18361 taatgcaata aatggataga tagttacttg ttttgcaagt atttataaat aatgagataa
    18421 ctgataacta ttaatattat caggattaaa atggagatat acagccaggg ctgatccgga
    18481 atcttcattt tgtcacggcg tttcttcttc ttttcggttc ttttcttcag atcacggttc
    18541 agcatttcta ccatgtactg caactgctct ttgtctttaa actcctgtag tccttctgcc
    18601 gcatcacctt caaaatcgct ttgcagtaat tgcttttcca cttcatgttt tttttctgca
    18661 gatagttttc cctgcaggta taacaataat gtttcctggt caacatctgt ggagagatta
    18721 gataatatgt ctttataatt ttctgacatt ttttatcgtt tcgctttttc ttgcaacttt
    18781 ttctcgatca gtaattttaa atttcgtttt ccgttttgta tataactttt cacctgcaac
    18841 atactgaaac cggttgcttc actcacttcc tggtagcttt ttttctgcag gtaaaacaaa
    18901 gttacacatt gctgctgctc cgggttcagt tctttgagcg aaagatccat gagctctaac
    18961 gtttgatcat tttccaataa agcctgccgg tctgtttctt ccccgggttt ggttgccaac
    19021 cggtcattga tctctgttgt aatctttcct tgtttttccc ttagtttcat caggcaatgg
    19081 tttttggcta ccgtatacag ccaggattta aaatattcta ctttatattt ttgaagttcc
    19141 tgaatcactt tcaaaaatat ctgttgtacg ctgtccttgg cttcttcttc gttttttaaa
    19201 tatttcattg atacacccag caatagaagt gtataccgct gcaataatat gcctagccat
    19261 tcattattat ggtcggcata aaattgttca agcaattcct ggtcggttat atgctggtat
    19321 ttatctggtt tcaccagtgg aaagtaacta tttgttaatg taagatgcag gatttgatga
    19381 tttcaacaaa atttacgctg atacatttac tattttcggc aaaatatttc aaatggacta
    19441 tgctgttgtt gccgtacctg cggcaccggt gaggcgaaag ccccgtcaca ataaggaaat
    19501 ggtgaaccaa ctgctttttg gggaaacggt gaaagtgtta aaagcaagag gtgatttatg
    19561 ggtgaaggta cgcagcctgc atgatggata tgaaggatgg atgactcata cattgctgga
    19621 agagatcgat gagaaaatgg cgagtcagca aagtgctttt atcactgctg atgtaatgag
    19681 tatcatatct attggcgata aaaagctcca tgtgcctgcg gggtcatcgc ttccattttt
    19741 tgaaaatggc atagggaagc tgggtaagct tgaatattat ttcaatggaa atgcatacaa
    19801 ccggaatacg caacaacctg gagacgccct ggtaaatgaa cttacctcag catggctgaa
    19861 tgcgccttat ttgtggggcg gccgcacacc attgggagtg gattgcagtg gttttacaca
    19921 ggttattttt aagttatcgg gtattgatct tccccgtgat gcctggcagc aggcccagga
    19981 aggaaaggtg gttaaaaaat tgagcgaggc acaaactggg gacctggcat tttttgacga
    20041 caaagaagaa atagtgcatg ttggaatttt attggataat caaaagatca ttcatgcatc
    20101 cggcaaagta agaattgata ctattgaaaa gaagggctta atcaatacag ataccgggaa
    20161 aagaatttac cggctaaagg ccatccgcag gtattggtaa gtaggccctg ttttccacta
    20221 cagtaaaacg tacagttatg gcaaagatgt tttcctcatt gttcttactg gtcgaccagg
    20281 caatagtaag tggtgaaaaa ataaggctgc tgagtaaata gtctcttatc tcccctgttt
    20341 ttttgtcttg taattttaac tcccggatgt taactccgta agggcctttt ttgtagcggt
    20401 tacaaaactt atcaaactct ttctgaatat cattgacacc ttctttaccc ggcggcgcat
    20461 agccaaccag ctggtaaaga aaaatattat taacctgtcc tgttgaatct gttatgtgct
    20521 cccgttctgc caatatgatt ttagttcggg tagttttgaa gaaaaatggc gagtgcgaag
    20581 tgtaagtacc ttcgagaaag aagagggtgc tatcggtttt tttattgatt gttttattgg
    20641 ttagtaacgg gtcatccatc aggcgaatca ggaagctact gaattcttta ttgaagggat
    20701 tgatacggta ataggtctgt gcaatattat atactttttg ggcatgcgca gttgaaatac
    20761 ttaatacaaa aagcagcagg taaaactttt tcatcaaagt aatcattaag cctgatgaat
    20821 gtagcaaata aataggtgca gaatgataat tgggttgtta aaaaatccct ctcagccatt
    20881 ttaccagcat ggtatcccag ttcagcattt ttaatgcagt atagaacagg ataatggcaa
    20941 atatcttttt caatacatcc tggttaattg ataaagcgat cttgccgcca aaaaaaccac
    21001 caattacaaa accggctgcc aaaagtccca ccactttcag gtcaataggg gtacccagtt
    21061 tctggcattg gtgataatag taaatggaag cgagtattac cacgggcaaa gtgagtacgg
    21121 ccagtgaagt gccttgtgcc tggtgttgag tatactgcat aaaaaaaacc aatgccggta
    21181 ccataataat accaccgcca acacctacta acccactgag tacacctgca gacaaaccga
    21241 tcagcagggc tgtaattatt tgttgagtaa gcatagtatg agtttggtta ttattcaccg
    21301 gttatttctt aaatgtctca ttgtataaat ggatggaatc ctttatgatc ttataggcga
    21361 tcagctgatc ctggaacgta tcaatttcca cttttttatt ttccagtacc ttgtattgtt
    21421 caaaaaagtt ccttaattca agcaggaagt gctggggtag ctcttccatt cttgttatat
    21481 gattgacgga agggtctttg gatgcgacgg cgattatctt atcgtcttcc tgcccgctat
    21541 cgatcatctg catattgccg attacatttg cttctacgag gcaaagtgat tgaatggatt
    21601 gggaacaaat gaccaggata tccagcgggt cattatccag tcctaatgtt tgtggaataa
    21661 aaccataatt gataggatac tggaaagaag agtaaataac cctgtccagt tttaataaac
    21721 ctgttgcttt atctacttca tatttggaac gggagccctg tggtatttca atcagcgcat
    21781 tgacggttgc cggtgctttt tctccgtagg tggcgccatg ccatggatgc aaaacattta
    21841 gcataatgat agttttatct ggttaatttc attccggcgt aggttgcaac taagccaata
    21901 agtacactag cggcaacata tgaaaaaaag agacctgttt tgttttcttt tagcaatcct
    21961 attccttcca gcgtaaaggc agagaaggtg gtgaatccgc cacaaagccc tgtcatcagg
    22021 aacagtttcc agtgttcatt actgtcaaaa gaacggaagc tcatgcccca gaaaacaccg
    22081 attaaaaaac aaccggtaat atttaccgca aatgtgcccc agggaaaaga atgtgaatac
    22141 acttcagcga accatcgctg gcaaagatac cgggcaatgc tgcccaggcc accgcccagt
    22201 cccactaaaa gaatattttt gatcatttca cagtatcgtt ggtaattact ttgttcaaaa
    22261 gggtatctgc gtcatgctca tacaggtatt taagatccac tacccattga ttatcggact
    22321 ttattacccg gagcgtatca tgatcatttt taaaagaatt ggaataaatg acaaatgagg
    22381 tagagtcatt tacatcattt accgtatgaa taatgatggt agagcccttg tatccatctt
    22441 tcactgcctg gtcagcattt ttgtaggatc gttctgccac atccaggtaa ttattattga
    22501 gagagtcctg cagcatatag ctcctggcat cgttgaattt tccatcaagt gctgagcgga
    22561 taaaattcct tgcggcatca atattatttt ccgactgtgc tgatttatct tttatatcac
    22621 aggagtatcc gacgaacact aaaccgagga ataaaataat gattctgata ttcatgattg
    22681 caaaatacta aaagtttgac ggctctaaac aaaaacaccc ctctaaaagg ggtgcttttt
    22741 tataaattgg ttctggttag ttctgcttca ttttttccag ttccttcatc gcatctttca
    22801 tctggtccat ttgttttggg tccattgatt ttaacgcact atcaatggtt tcaaaccctt
    22861 ttttaagtga atccatattg aagtccttca ttttgtcaat atcttccggg ttgatttcag
    22921 tgccatcttc tttggctttg tccataccca ttttcattaa tgttcccata ctgaaatcga
    22981 ctttccagct gccatcttct tttttcagcg ggaaatctac tgtctcattt ttctttttat
    23041 tcgtaactga tacggtggca ttactaccat caattttggc atcgccaacc accatatttt
    23101 taaaatcttc agcgggatct tcttctgttt tttcagtgcc tttcattttt tcggccatat
    23161 ccatggcttt tttcatcata tccattgtgc ttttgctgtc tttggtcgcc agtttagcag
    23221 cgccttcaag gtctttttta gccatccttt caaagaaggc aacaagtaca gctttggggt
    23281 tatctgcaac accgtctttg cctttacatc ctgttattat cagggcggtt actgcaacag
    23341 cgaaaatgga attcattact tttttcatgt gttaaatggt ttaaggcttc aaatgtagca
    23401 attacaggta taaaagatat caaagtttaa aactttcctt tttaaccagt ttacagtcgg
    23461 gatgacggaa aatatcatca gtggtaacgc caaacgggat ggtatcaagg cgtacataat
    23521 ccgtataggt gccatgaact gctttattca gtgatttaaa tacaaattca gagcaataca
    23581 ttttatcgtc gctccggaaa ttgaaataca tatcaaattt taaaccctgc tgatagtagt
    23641 taagtactgt ttgttgcatt aaccggagtt gcccgctggt taaaccgtaa cgataaattc
    23701 cgaaggcatc attatctttt gggttacaaa aactatcaat cggctcccgc agtaatttct
    23761 ggctggggtt ataactgccg cctaaggcat gataaacaaa tacactgtcg ttttcaataa
    23821 aaactatacc acaatgcgag taggaagtgt ctttccggtt catactccgc gccgcccggc
    23881 ttacatcgtc tatcccattt ctgaaaataa tatccccgct tttgatgagt gactttgcac
    23941 ggtctatttg ctgaaaattg gcagccagca ttgctggtgt cagttgcgta tcctgcgaat
    24001 ttttgcagga tgaacataaa aaaagcagga ataaaaagac aaattcagtt ctcttcattc
    24061 ctgcaaatta atataagttt ttccgctatt ggggtttatc cttcttcagt atagccggtg
    24121 tttgccggtt ggcatattct tcgttttctt tttcatgttc cttgttataa gcctttatga
    24181 tggccttcac caggcggtga cgaaccacat cttcttcgtc cagctcaata tgggcaatgc
    24241 catcaatatt tcttaagatc cgggtagcca tttgcaaacc actccgttgg ttcctgggca
    24301 ggtctacctg ggtcatatca ccagtaataa tagcttttgc attagcgcca atcctggtta
    24361 gaaacatttt taattgcagt tctgtagcat tctgggcttc atccaatatg atataagcat
    24421 tatccaatgt gcggccacgc atataggcca atggagcaat ttctattgtc cgggtggtca
    24481 tataataacc caatttgtca gcaggtatca tatcatccaa tgcatcgtac aaggggcgca
    24541 ggtaagggtc gatcttttct ttcaggtcgc cgggaagaaa accaagactt tcacctgctt
    24601 ccacagcagg tcttgtcagg atgatctttt tcacctgttt attttttaat gcccgtaccg
    24661 ccagtgccac agcagtatac gttttgccgg taccggctgg gccgatggca aaaacgatat
    24721 cgttcttatc cgattcctgt accagtcttt tctggttagc ggttctggcc cttactgatt
    24781 ttccgttggg accaaatacc aatacttcat tggggttctt ctctttaaaa tggtcgatga
    24841 cttctgcatc atctccaccc aaaacctgtt caaaataatt ctggctcatg tgaccattcc
    24901 gttccaggta cgaaacaata agagaaattt tttcccgggc agtttcaatc tgttcaggtg
    24961 ccccgctcag ctttagctgg gtgccgcggg agagtatttt cagcagggga aatttctttt
    25021 tgaggatgtc gagcttaccg ttgtttacac caaaaaattc aatagggttt acggtttcaa
    25081 ggttgatgat agtgtctgtc aatgcacttc ggtttatgtt tctactaagc cggcgaaggg
    25141 tagtaccgga ttattttgcg tcttctgccc gattacagaa gctttaccct tctgcatcaa
    25201 tattacttag caatattgtg acttcctaaa gttacttatt cacaggttgt gaatagagtt
    25261 ttggcggatg aaagtttagt taatgatttt ttatgtatga tacaggtcag ttttaaagat
    25321 gcctgatatc agccggaggt tttaagataa gtgataactt taatgcatgg cacaagaaat
    25381 aaaaccaata aaaagaagca gtcaactggc tccccttagc cgggagcacc acgatggatt
    25441 attgtttgcg tggaaaataa aacaggggtt ggacaataaa actcccctgg acaagctgag
    25501 aaaatattcc ctgtggtact ggcagcagca catcaaaccg cattttttcc aggaagaaaa
    25561 aatactgatg ccttatatgc cagcgaacca cccgatggca aaacaactaa aggaagagca
    25621 tgaccaaatc agggaactaa ttcttagtct cgatgctgaa gctgacgaga gggatttcac
    25681 catattagca gacctgttga ataaacatat ccgctttgaa gaaaggcaac tctttgcatt
    25741 cctggaggag atactaacgc ctgaacaatt ggataaagtt tttcagcaac tggaagagca
    25801 tccgctaacc tgcggggaat ggaacgatga attttgggtt aaaaagtaaa ataatactga
    25861 tgcgccctgg atgattttaa gtacctgtat caagttagcc agaccacagg atttataaga
    25921 atacaattga catctttcct tatactgcag caacggagta taaattattg cttatttccc
    25981 ttttgggcga cgggaattca tttttccctg ttaaagaata tgaaaagatg ataataaaca
    26041 gggattacag ggaaagaatt acaaattttg ctgtcgaatt aatttaatgg ccggaagatg
    26101 aaatttaagc tctctctatt attactgttt ttgttactgg ctatcttttc acagggccag
    26161 ttgtacaggg tttccggcgt tgtgatggat agtaaaaaag aaccggttcc ccttgccagt
    26221 gtagaaataa aagaattgcg gaagggttct gttaccaaag acaatggctc ttttgaattt
    26281 ttcctggaaa gaggaaagta tgaccttgtt gtgagcatga tcggtttcag tactaaagtg
    26341 gttactattt atattaataa tgaagacatc gttgagaatg tggtacttga tcccgatgag
    26401 tccaatgttt tatcagaagt agtgctaaaa gtgaaaaccc gggacagggc agaagagctg
    26461 atccgcaatg tgatcaaaca aaaagaaaca gtattgggtg ccataggctc ttattcctgc
    26521 aatatttata taaaagcttt tcaactggat tccggcttca ccaaaaagaa aaaggcgatt
    26581 gaagcagata caatcattaa agaagattat gagggcatgt cgcttacgga agtatcactc
    26641 cggcttgacc gcagtgccgg cgggcagata aaggaagaac gggtgggggt gaaaaaaatg
    26701 ggaaatacag aaagcctttt ttacctctct actacagaag gagattttta tatgtttaac
    26761 aacatgatac aggcgccgcc tgtatccaaa ataccatttg tatcgccact cagttatagc
    26821 gggttgatgg cctaccggtt caaaacacta aagattgacc gaaccataaa acccaaagtg
    26881 tacactattg ctatccggcc aagacaattg agtaatgcca ccattgaagg agaaattacc
    26941 atacaggaca gtacctggga ggtattcagc acagtgttca ggttgccaca ggcccatatg
    27001 cctgaatatg attacatgga agtgaagcaa cagtatggca aagtagggga tagtgcccgt
    27061 atgatcaccc gccagcagtt cagttattat acgaaagtaa aagggggcag gcggtatggt
    27121 gaaacaacag ccatttattc agattttgag ttacagaaaa attttaaaag agggcatttt
    27181 ggtaatgaag taagctctac atcgcaggaa gcatatgaaa aggattctgt attctggaaa
    27241 agcgtaagaa cagaacctct ttccaaacaa caggccttat ttgccaggta ccaggatagt
    27301 gtgtatcggt atatgcgcag tgaaacttat ctcgattcga tggatagggt gctgaacagg
    27361 atcacctggc ccaagatgct gatcttcggt caggtattta atgaccacaa gaaagagagg
    27421 atgtggatac tgcctcccat tacttcattg atacaacctg ttgcctttgg tggtgcccgg
    27481 ctgaaacttg caggagctta tcgtaaaacc tattcatccc ggaaaaattt attcctggaa
    27541 gcagatatta gttacggctt tcgcaataag gatatcaatg gaaccgtgag tatacaacgg
    27601 atgtacaatc cgtttaaccg gggacaattt aaaataagtg cgggccgcaa ttttgaattc
    27661 atttatcccg gggatgcctg ggtaaatgtg ctgaaacgaa gcaatattta cctgaacaat
    27721 tcgctggaac tatcgcatga acttgaactc ttcaacggtg taatgctgct gaataaatta
    27781 gaagttgcac tgcgccgttc ggtaagtgat tataaagtgg gtaacaatgc cgacagcatt
    27841 tttggcatcc ctaatgaacc accggtggag tttgacccgt ataatgctac gtataatgag
    27901 atcaaacttt actatacacc caatatgaag tacatcaggg aacctaaaga gaagatatac
    27961 ctgggttcca aatggcccac tttttatgtg acctggcgga aaggggtacc caaattgttt
    28021 aacagtaaaa ttgattttga ttacctggag tttggcatgg agcagaagat caacctgggt
    28081 gtggcaggca atacatccta tatcattaag tcgggggatt ttaccaacac caatgatctg
    28141 cgtatcattg attataaatt tatgcggcag ggcgacccat tattttttca gaatccacaa
    28201 tatgctttcc aggcactgga ttcaactttc ccggtatttg accggtacta ccagggaaac
    28261 atagtgcatg aatttaacgg ggcgctgatc aataaaattc ctttctttaa aaagctaaaa
    28321 ctgcaggaag tggcaggcgg gggattttta atcacgaaag acaggagcct tcgctatttt
    28381 gaatttttta caggaattga aagggtattt aaatggcctt tcaatccatt ggcaagagta
    28441 aagctaggtg cctatgtggt tggttcggta gctaacaaat tcaataaccc ggttcagttc
    28501 aaaattggat tgaccacatg ggacaggttc aggaacaggt ggagatgatc aatttaattt
    28561 ccttacctga ttctgaattc ataagccagc cttatagaag caaatgccct ggtacgatct
    28621 gtaacccagg cttcatgacg tatgctcaaa tcaaggctgt tattattttt cttgttagta
    28681 ataatctcat ccccgataaa aaaagaatat acaaaaccat tgtttggttc aaaacccgat
    28741 tgatttagat atccggcgtc acctccgaaa tagaaatttc caagataata tcttaaacct
    28801 gctttaatgg gtattcccca tacagttctg gcatcgggat tgagcagact tgtttttcca
    28861 gataaagaat aagcaccggt ggaaaaaata aggctggtat gcttgtcaaa taaatattct
    28921 gcttttatag tgcttccata tccccagcta tgtgtttttc taaaataatt ttcagggtaa
    28981 atgacatctg ctcctacact taaattccat gtattccttt tatttacttg tgcgatgata
    29041 gatgtggtac aagtaatcat ggaaacaaat aatatagcta ctgaaatcgt tctcatgatg
    29101 tttttttaaa taacatcagc gctttacgat tgttcagtta tgtaagcaat aaataataat
    29161 aatgttgaac ccggaagtta gattgaatca ttaccaccta gccacaatct taaaattcac
    29221 caatcgtggt gttaaccggt taggcatcag gtacaccgtg ttggaaaaat cttttacaaa
    29281 aagataggag atcacattgg cccggtcgat gatattaaat acttcaaggc tggcccagat
    29341 attatcaaaa ttgcggaaag gagagtggct gcgacgtttg ctgcggtcat catctaccag
    29401 caacgcactg aaaccaatat cggcacggat atagggttct atcacggctg cattgcggta
    29461 acgcaccgcc ccgggaatat tataaggaag attacttccg taaagcaggt tcaaaaacat
    29521 tttgaaattc ttgttggtag gcaggtaatc ctggaaatac attccaaacg taatgcgccg
    29581 gtcagtagga cggcgcagcc agcctacatc aaaacgggtg ctgtccacta ccacctggtc
    29641 ggggctttgt gcattgatga attcaccggc tgcattttta taacgatagt aatgatcacc
    29701 gctgatgtct tccgcagttt tcatgatgcc gagactgatc cagctttctg catcaggtac
    29761 taattcgcca tgcaggcgca tttcgatacc ggtggcatac gctttagcat tgttttcgcc
    29821 aaaataacgg atacggacat tgtccacatc ataaggcact acatccgtca tgcttttata
    29881 gtaagcttcg gtggtccacc gcatgggccg gccaccaatg ccaataaaat tataatcaaa
    29941 cccggctacg gtttgccagc ttttttgtga ttgcaggttc gtattcaccg taccgttata
    30001 tcttctcagt tcccggtaga atggtggttg atgataaacc ccggcagctg cccggaaaat
    30061 aatatccttc tttgaccggg gtttccaact ggcgctaata cggggagtga ttaaaaattc
    30121 ctggttgagt gaattataat tgaaacgtac accgccggtt aaggtgacgg agttgctgga
    30181 atctcccagc agaatattat cctgcaggta accggaaaat ttattgattg caaggttggc
    30241 attggatttt atcactttgc ttaattgcag catgttgggt tggtatggca atgcataccc
    30301 ggcagaatcc tggaactccc attcatttaa tttatctgca atggttgttt tgtcatagcc
    30361 gattccccat tgaaaagcat gtttgccctt gtcgtaattt cctttatgtg atacattcca
    30421 gttttcaatg ttcagttcgt ttcttgccca gttttgatat acaccggcgc cgagaggatt
    30481 tacaattaaa ccataggtgg gatttcgttt atcaaaatcc ctgtcgccaa ataaatacgt
    30541 tccgatgatg tccacatttt ctgcttcatc attttcaaac ctggatgcca tccattttag
    30601 tttcagtttt ttggaaggct ggttgatcaa tgaaaaaccg agcatattgg tttgatacct
    30661 gtctttctcc cggccttcaa aataaatgtc gacaccaata gtagcgctgt acagcggaga
    30721 gaatacggaa gtggtgagct ggctgaattc agggatcagt gtaaattttg tctgcgatat
    30781 attgctgagc agctctgcct gccatttagg actgaactgg taattgagca aagcctggaa
    30841 atcggcagag gatgggacat agttaccctt tgtatcctgc cggcttaata aatttctgtt
    30901 gcttcggttg cgaaccccca acaggtaact gaattttgaa tttttgctgg tgccttccag
    30961 ttgtaaacct tgttctaata tcccaacata agcagaaccc ccaaagcgtt tcggtttttt
    31021 gtattgaata tccagcacac tgctcatctt atcgccatac tttgcctgga aaccaccatt
    31081 gtaaaaactg atattacgga ccatctccgg gtttataaaa ctcaatcctt cctgttgccc
    31141 gcttcttact aaataaggac ggaacacttc aaagtcgttt acatagatga ggttctcatc
    31201 ataactgccg ccacggacgg agtattgaga ggtgagttcg ttattggagc ctacaaatat
    31261 tttgatcagg ctttccacgc cagtcaccgc cgaaggaaga ttgagtatag ttttaggatt
    31321 aggtcttatt aaacccactt cccgcctgtc ccgctgatcg gtaatgatca cttcctgcag
    31381 ggtgttctca cttttttcta atcgtatcgt gatggtttct tcttcttctt cgctcagcag
    31441 gaaatttttt tgttctgcac ggcggccggt gtaagtgaaa attaatgcga aagctttatt
    31501 ggctgcaact tttatgcgga aataaccaga gtcattggtg gtaatgcctt tggactggcc
    31561 cagcaccacc acagaaactt tcggtagcgg attctcattt tcatccacca ctttgccgga
    31621 aacgtaagct gctttttttt gagagaagga aagtatcgaa agcaataagc aggtggctgt
    31681 tagtagtatt tggcggaggc tgattttcat aagaaatgat aagccaagat acgaattgtt
    31741 tgatgctggt aaaacacttt atttcgacag ataaaaataa ttataccggg gaggataact
    31801 taccaggttg ttgatggtaa aactggtgat cttatttaca caacctactt ttacattgtt
    31861 ccagaaagca tgtctgattt cgccggcctc ttcttcaata gcagaaagtc tgtatagttt
    31921 tatggctccc ggaaaatata attcatagtc atattgactg gtgatgagcc cggttgatgt
    31981 tgtgtaagca agatcaaggg tatcgttggt ccttgtaaat cccatatcca gtaaaaagct
    32041 gtcttttaac gttgaaaaat tactgccctt ttcgtagcgg cgaagaataa tcgtatcgct
    32101 ttcctggtca gaaaaggaga taagcccaaa tgacagggaa gcattcatac agggagtcct
    32161 gctacagtta taaaaaagga tagtaaccag gataagtaaa gccgaagcaa gaagatgctt
    32221 aaacatacaa tgatgacaaa taggaattgc ttaaagctgc ttcaattgcc tgatggtcgc
    32281 ttttggatca gttgatttga aaacagtatt acccgccacc agtacatcag ccccggctgc
    32341 aataatagaa ggtgcatttt ccagtgtaac gccaccatca atttcaatat gcacttttaa
    32401 tccgctgtca tcaatcattt tgcggagttg ctttatttta tctaatgtgt aaggaatgaa
    32461 tttctgtcca ccaaaacccg ggtttacact catcaggcat acaaggtcaa tatcatggag
    32521 tatatcactt aagcctgaaa caggggtgtg gggatttaca gccacaccgg ctttcatacc
    32581 cagtgcttta atctgttgaa tattgcggtg caggtgccgg caggcttcca catgtactgt
    32641 taatatatca gcaccggcat ttttgaattg ctctgcatat ttctccggtt cttctatcat
    32701 caggtgtaca tcgcagacct tggtggttgc cttccggata aactctatga tcattggtcc
    32761 aaaactgata ttgggaacga aacggccatc catcacatcc aggtgatacc agtctgcttc
    32821 actttcgttc agcatggtac attcctgatc cagtttcaga aaatcggctg caagcaatga
    32881 aggggcaatg ataggcatga gttatttatt tgcgctgcaa gatacttcta tttgagttta
    32941 tctgccgagt ctttagcagc aataaaagta ttgtcgaggc catatgccct ttgataattc
    33001 agtttggctt cttcacgttg ccccatagcc tgctggcatt tggctatcca gtaataatta
    33061 tctgcaaact tcggtgagat ggtaagggcc aggttgaata ctttcagggc atccgggtat
    33121 cttttcattt catatagtaa agaacctttt tctatatatc catccaggaa atagtaatca
    33181 tgctttaccg cttcatcaaa taaggaaagt gcttttgttt tatcattgat attggaataa
    33241 tagatgcctt tgtaataata aggttctgcg tgaacacctg atgagtcagc tcttatcaga
    33301 gaatcgcata aggcgagcac ccggctgttt tttgcttctg catattttaa agcaagtata
    33361 taattcagct ccacatcaaa tggggtaagt gtataagctt tttccaggat agccgtggct
    33421 tcgttcgtat ttcctttttt agccagcagg tccgccttca tttttaatac atctacctgt
    33481 tccggatttt tttccaaaat catatcacct atcttcaatg cttcctccgt tttattggcc
    33541 gcatcataac cccgggcaag actaagttgt aataaaagac tgttgggaat atcttttatc
    33601 gccgtattta aaaatgtaat ggcctcttcc ggttttttat ccagcagcaa cgtagcaaca
    33661 cccaatgcgt attgttcttc ctttttcagg gaccatgctt tctggaaatc ggccagggct
    33721 ggttcgggta aactgtttgt atttagtaag atggcccgcc ggaaatagaa ttcagctttt
    33781 cctggttcct gttttatact gtctgtaaga tcagcatagg gaggcctgtt gaggatttca
    33841 tcataggcag ttactgttgt gtcactatca ttacaggcac tgattaaaat cagcatccac
    33901 agaaccgggg ccattatttt tgcaacctta ttcatatttc aaaactaaat tattattagt
    33961 ggcgaggtat gacaatagtc tgacaaacca acgtaataac gaaaatacct ttgcacccgc
    34021 cagccatcgg gcaggtttca aagaaacgac attgtatgaa aaaacttgta ttatccctgt
    34081 taattataac cggaatttcg tttgctaatt cttcctgtaa aaactcctcg aaaatagctt
    34141 ctactgctat acaggtggca gatacattac gttacccgga agagtctcat ttgaaaaata
    34201 ttcagcaact cacttttgga ggtgataatg cagaagcata ttggagttat gatgggaaat
    34261 accttgtttt ccagaaaacc aacccgaaag aagggatcaa ttgtgaccag atatttgtag
    34321 gtaaagtacc caccaaagcc ggtgagaaat ttacttataa aatggtaagc accggtaaag
    34381 gacgtaccac ttgtgcctat tttactaagg atggcaagca tatcatttat gcatccactc
    34441 acctgggaag tgatgattgc ccgccggtac ccgacaggtc aaagtatggt aataaatata
    34501 tatggccgct ctacagcagc tatgatattt tcatggctga ccttgatggt aagatcgtta
    34561 agcagcttac taattctaaa cagtacgatg cagaagctac tttatcaccg gatggtaaga
    34621 agatgatcta taccagcacc aaggatggag atattgaatt atacattatg gatctgaaaa
    34681 caggagcaga aaaacggatc actaatacgc tgggatatga tggtggtgcc tggttcagcc
    34741 cggatggtaa aaaattgatc tggagagcct ccagaccaaa aacggaagcg gaaataaaag
    34801 agtataaaga actgctggct gaaaatatgg tagctcccac caatatggaa gtgtgggtgg
    34861 ccaatgcaga tggcagtaat gcaagacagg taacatctta tggccaggct aattgggcgc
    34921 caacttatat gccggatagt aaacgtatca tcttcgcctc caatcatgaa tataaaagag
    34981 ggttcccctt caatctatat actatcaatg aagatggtac caacctgcaa aaggtaagtc
    35041 gtgacaaagg ctttgatgca ttcccgatgt tttcgcccaa tggaaagaag attgttttct
    35101 ccagcaaccg taataacggg ggcaacaggg acaccaatgt attcatcgca gactgggtgg
    35161 aataagattt cggggtttaa aaattagcct ttagatttgt ttcaaatctt tcatcatgga
    35221 aacaggaatg ttgcatttgc ataatgtgct tcgttgggtt attttaattt tgttactggt
    35281 aacattatac caggcatttg caaaaaaaga aagtatacgc agcagcagct tgtggctgat
    35341 gatcgctgca catactatgt tgctgatagg tttgtaccag gttattgccg gaaggttcgg
    35401 catattaaaa ggcttacccg aaagtgtacc atcattaatg aaagactcct tttaccggtt
    35461 ttattgggta gagcatccca ttttaatggt ggtggcaatt gtattgatca cactggcccg
    35521 tggcaaagta aaagcgctga attataaaac agccggctgg ttattactca tagcccttat
    35581 tttcatactg gtagctattc catggccttt ccgtgaagtc ggtgtaaacc gggcatggtt
    35641 tccgggtatg taaaaaatta agaagctgtc acaaaaaata tgttagcctc agtttgatat
    35701 atgtcagcct gagcttgtcg aaggctatcc gtttgattgc caatatttcg atatcccgat
    35761 agctatcggg acaatatgac aatcaaacat accttatacg acagtctctg caagtaagat
    35821 gatttttcaa attcggaatt cttaattatc cttttaaggg aattttaaag aggtttattt
    35881 tttccccaac tcaatcactt cgcagccttt catatcattt ccatcaatta caaaacgaat
    35941 aagggtcctt actttatgcc agccctgttt gcccgcagcg ccgggattca tgtgcaggca
    36001 atttatttta tcatcataga tcacttttaa aatatgggaa tgaccactaa tgaataattg
    36061 cggttggtgg attaataatt ctttttttgt ttccggattg taccggggag gatagccacc
    36121 aatatgccgc atcattactt ttacgtcttc acagttaaaa acaagttgtt ccgggtatat
    36181 agttcgaata tcatttccgt caatatttcc atacacagct ttaaggggct tgaatgaagc
    36241 aagttgattg gctaattcaa tagtgccaaa atcaccggca tgccatatct catcacaagc
    36301 tgcaaaattt ttaaaaattg cttcatccaa aaagctatgg gtatctgcta taagtccgat
    36361 tcttgtcatt tcaggattga aattagttgt aaatttgaat atgcctttta agcgcaactt
    36421 cacctattat attgataaaa gaaaatggaa gtatatcttc ctgcttgcta ctttagaact
    36481 ttcccaaaaa aagtttttgc taactgctaa aacctaaaag ctatcagctt aaagttaact
    36541 ttcaactcta aactctaaat tattcccatg cctcattatt atacacttgg aaccattccg
    36601 cacaagcgcc acacacagtt ccgcaaacct gacggaggtt tgtacagcga acaattattt
    36661 tcgactgaag gcttcagtga tgattatgca ttgctgtatc actgtcatcc gcctacccag
    36721 atcattaaga cagaaccaca ggttgatgta tcaccacagg tggctgaaga gaaaatgctc
    36781 aaacaccgca gcctcgaagg ctttaaggta aaaccggtaa aagattacct gcaaagcagg
    36841 gtgcctgtac tggtaaacag tgattgccat attgtattgg cagcaccgca ggagagcatg
    36901 acagattatt attataagaa caccgatgcc gatgaaatga tctttgtgca tgaaggcagc
    36961 ggtatcgttc atacacagta tggcgaactg cctttttctt acggcgatta tattgtgttg
    37021 ccaaggggaa cgatctacca ggtcagtttt aatacaccgg ataaccggct ctttattgtg
    37081 gaatccttct caccactccg ttatcccaaa cgatacatga gtaagtacgg gcaactgatg
    37141 gaacactcac cctattgtga aagggatatc cgtccgccgc aggacctgaa gacgtttgat
    37201 gagaaaggcg attttttaat taaggcaaag aagaaaggaa tgctatacgg cctgcattat
    37261 ggtacacatc cattcgatgt catcggctgg gatggttgtt gttatcccta cattttttct
    37321 attcatgatt ttgaacccat cacaggaagg gtgcatatgc cgccgcccat tcaccagacc
    37381 tttgagacca gttcgtttgt ggtttgttct tttgtaccgc ggttatatga ttatcatcct
    37441 gatgccatcc cggcgccata caaccacagt aatatagata gtgatgaagt gttgtattat
    37501 gtggccggtg aatttatgag ccgtaagcat gttaccaaag gcatgatcac tttgcacccg
    37561 gcaggcatac cgcatggtcc gcatccggga gcggtagaaa aaagcatcgg tgctaaagaa
    37621 acaaaagaac tggcggttat ggtcgatacg ttccggccgc tgatgctcac caaacaggca
    37681 ttggagatag agaaccagga ctatgttatg tcgtgggcgg aataaacgta atgtcacccc
    37741 gggcttgacc cggggtcttt ttattatctg agcagcgtat ttttttaaac cgctaaccct
    37801 ttgttcaaag ctattaccgc cagtcctgtg cgggtgcaaa cttcaaatcg gtcaaagagg
    37861 ttattcctta aatattctat ttgcctaccg gtcagtttca tttctctggc aatttcttta
    37921 taggtctttt cgctgcatac cagttttaaa aatcgaacat cagtttcgct caggtcattc
    37981 tgtatcgtga gggttcctgt ctgcatggct tttttcacca tcttggaggc agtatggtcg
    38041 ctgaagaagt aaccgttacg gatcatttct gctatagctt tctttaaacg ggctatatca
    38101 tcatttttat ttacaaagcc ctgtgctcca aattttatta tgcgccatac cagttcttcg
    38161 ctatagtgta aggatatgac aaggagcctg atctccgggt atttttcttt aatggctttt
    38221 agtgtttcat agccattcat ttccggcatt tcaagatcta ccagcaccag gtcgggaaga
    38281 ttttttgggt gtagcttctc caataattgt cttccatttg cggcctgtat gactacttta
    38341 caattttccc aggtatctat aagggcacaa agtgattccc tgaaaaggtg gtgatcatcg
    38401 gcaactgcaa ttttcaccag gctgttttca ttaagaaacg gcataggtaa gggtaattaa
    38461 tagtgaaata atcaatacgg aagaagtata ccggcaaagg cggcggggta aaaatcatag
    38521 tataaggttt ggttaatact tcaaggtata ttaatagggt gaaatttcac ataccacttt
    38581 gtggtaattc tcacacgatt ttgtgaaaat tcacaatttt attccggctc ttttcttaaa
    38641 ttttgtgttg ttgaggttct ttgacaggca ggcaaagctg ctaagggtaa actcttgcaa
    38701 gacggaaaag ataactaatg tgaaagttca caaccggtaa ggagagaaaa agcattacta
    38761 attgtgagaa cacacttgtt tcaggcttta tgggtacggt acattcggtc atcattgggc
    38821 taccaggccg gcggtaaccc caatgctacg gggaagccga aaaccgggta ttgaagagag
    38881 taggtgcaaa taaataattt tatgaaaaag atactaggag tattgattat caacctgatt
    38941 tttttctgtg gagctttgaa gtcgcaagta acagcagaag ctttccctat gaatccgcaa
    39001 tccggttctc ataattattt tggagtaaga gtaacacttg cccaaaccta cactgaagac
    39061 ataacagtta ctggctatat ttatgacatg gggggaggtg caaatactaa taaccccttt
    39121 tcattgactg taacagccgg taacctgaca gcagaaacag cagctaattt ctatcagact
    39181 gatcccacag caacagctga agccacattg ggtactattg caactgttta tgcaggagtg
    39241 acaattactt atgaggctga tggatgtatt ttgaagttta atagcctggc cgatgcagat
    39301 gcggttttaa accatttgga ggcagatgat gataattata atgatgatta tgacagccaa
    39361 tacccaaacg ctacagaaga tgaactggac gatttggatg agcaaaataa cttcgataac
    39421 atgaaagtat acagagattt tgaagcactg ttcggtggat tttgttcaaa aagagctgaa
    39481 ttggaatcga tagaaaatac atggctttca aataatttca atggcacgga tcctgatgat
    39541 gttgatctca cttttgatga tgcagaaaac acaattttta atagcagcta ttcatttaaa
    39601 attggaacag atgtatacca attaacgagc acaggaatgt ataaaaatgg cattttacag
    39661 gatgaaggcg gaaactctat taaccgcaaa ccggataatt tcattgagct acccgaatat
    39721 ggtgtattgt caggcccttc atccttcccc aactataatg ggtcatttcc taacttttca
    39781 acagcaaatg cgctagtata tgattttttt acagtttgta aatcgaacaa aaaagataag
    39841 atatttcaag agtatgacaa tggcaataaa cgagtaaaag ctacagtttc aattacatcg
    39901 acagtagtac gaaacggagt acatggtaaa atcaaaaact ataaaagcaa aaacggaaat
    39961 tggaaaagaa gccgggcaca attggcagta tactgttttg gaacttacta taccaatctt
    40021 tgcgcagatt ctcatcaatt taccgataga aagccatcat caggaactaa aaaaagagcg
    40081 aaaatatcca caagaaggca ctcccccgga cacataccag tacaaggtac ggtttggaaa
    40141 gcttttacag gtgaagtcgc aatgtatgga gaagttggtg gttccgtacg ggggcagtta
    40201 acattaactt tttaaaatgt tgtaatgaca attcaaacaa cattaggcaa acaaggagga
    40261 attatacgca tcattcttgt ttgcctcttt ttgcaattta cagcacaggg gtacacccag
    40321 gaaacgaatt atgaaatcaa ggatcatcta tctcagaaaa ataatatcaa attacaaatt
    40381 ggctatgggg taattccaaa cggagttatt gaagtgaaag aggggattta tgacctttat
    40441 acaaaaccac acagtattta ctctgttgac ttaatttatc aagtaaacct aaatccatcc
    40501 tggtcagtaa acactggtat tggctttatg attattaaaa gtaatttttt tgagaaaatt
    40561 ccgatcaacg acctaattgg cacgggaata tcacgacagg aggactcgcc gccaatcatt
    40621 tattataagg gagcttatcc gagatttagt ctaccgattt tactacaaaa acgttcaaaa
    40681 attattaata gaagcttctt tgaattcaac ggtggtttca atatcaatta caacggattt
    40741 tcaaaagatg aaagaataat tatgaatgta gcagacagta ataatcagtc tataaatatt
    40801 tttaatgcta attataaaag taacaacaat cacaaacctt ggtttggctt gttacttggt
    40861 tgcggcaagg gctttttttt aagagactta aacgtagtaa cagtttcctt gtctgcagag
    40921 gtaaacacta caaaattttt atccgctgat tatgaaatta ttatacctaa taaaccaatt
    40981 agcacaggca gatattcagt caaaggacca cttctgcaac tatctcttgg atatgtattc
    41041 agagggacac ataagaataa tgattgaaat atttgaaaaa tgaccttact actttgtaaa
    41101 taaattgatc acttctttaa aattggctgg ctgactagtt caaaaaaaca tattagagaa
    41161 caagaaagtg aaaatctttt tgccaatatt attctggcta tggccggtgt ttgccttaac
    41221 ccaggcaaaa gacagtttgc aaaagctgca aagcaaaccg atgctgtcaa agcctgttac
    41281 taatattacg aaagattaca aacccgaaat cttcacttcc ggctttattg acatcgtaaa
    41341 caacggccag gtaaatgctt cagcacggtt tataagattg tacattggtg aaccaggtaa
    41401 atttgccatt cccgtttctt tttatggagg cgtaagtaat aatacttttc agaataattc
    41461 acccggcacg gtgttttcca aaagcaacag ccacctggta aaccagtaca ttaacccact
    41521 aagcggcctg attaatatca gcattgaagg ggtacagttt ttcaataaaa aagaaaaact
    41581 cactaaagct ggtttgttat accaggttgg cgaaagagta ttaagcggcg tccgcagtgg
    41641 cgagataagc aatccgctta caggcaagcc ggttaatttt ctcaacagct ttgcggcaac
    41701 aggtatttac tttcaaaccg gcgcctggga gcggaacaat actaaaaatg tgggggtgtt
    41761 ctggttagct tttcgctacc atacctgtaa gagtaatgtg aagcagatca aagagttttt
    41821 acctgcaatt gaaaccaacg gatggtacca cgggtggtct ggcgggtttg gtattgagat
    41881 caacaaactc gtcaatatca aagccatata ttacaaatac agcaaagcac cggagataga
    41941 atacggattg cctatttacc agttttcgtt tcagtattcg atgaagtagt tgttttatag
    42001 tctgtaacag ttgagtattt ttcaactgca acacgacttc ttttcctgtt agacaacctg
    42061 cctttcatac actcaatcgt atgttccaaa tatttttttg gaggtgatcc gtaaaatctt
    42121 atttttgtta accatttggt taaatcgtat ggttaaaaag caaaaagatc agactacgga
    42181 gcaggtaatc ctggcagctg ccaaaaaagt ttttgtgcaa aaaggaatgg ccggtgcccg
    42241 gatgcaggat attgcggatg aagccggtat caataaagcg ttattacact attatttccg
    42301 taacaaggag aagttatttg aggtaatctt cctggaggcg gctgaaaaac tttttccaaa
    42361 gatcaatgcc atttttgaag cagatcggcc tttattcgaa aagattgaga acttctgtga
    42421 tgaatatatt tctatagtaa tggataatcc ttacctgccg ttatttgtgt taaatgaaat
    42481 aaaccaggac ccggagtatt tcttaaaaaa ggtctgggca ggtaaggcaa aacccaaccc
    42541 ggtaaaattc ctggaacaaa tggagaagga agttaagaag ggaacgatca aaaagatcaa
    42601 tcccatgcac ttgctgatga acctgctttc catgacgata tttcctttca tagcgaaacc
    42661 aatgtttcag aaaaacctgg gcatgggtga agcgcagttc agaattgcaa tggaagaacg
    42721 gaaaaaagag ataccccgtt ttatcattga ctcaataaag aaataatttt tttacccgct
    42781 aatttattac cgggtttatt ctaatacatg atcatgaaaa aacatttctt acttgctgta
    42841 ttattactgc cggcagggtt atttgcgcag tctctgcaaa agataacgct tacagaagcg
    42901 tatgagctgt cacagaaaaa ttatcctgcg gtaaagcaaa aggatcttgt aaagcaaact
    42961 gctgccatta gtttggaaaa tttgcaaaag ggatttctgc cccaattttc tttgagcggg
    43021 caggcaactt atcaatctga tgtaacaaaa gttccgtttt cactgcccgg tcttaccatt
    43081 gaatcgcctt cgaaggacca gtataagtta gtggcagatg taagccagat gatctatgat
    43141 ggcggaatga cgaaagaaca aaaggcgttg cagcaactca atgccaccgt ggaagatcag
    43201 aaagtagaag tggagttgta taaactcaaa gaaaggatca accagctcta tttaagtatt
    43261 ctctatctcg atgaacagtt aaagcaggtt gatttagtaa aaactgatat tcaaatcggt
    43321 attaaaagag tagaggcgca ggtgcagaat ggtgtagcat ttaagtcgaa cctgaatatg
    43381 ctgaaagcag agttattgaa aactgaacaa agagctatag aagtaaaatc ttctaaaaaa
    43441 ggattgctgg atgcattgtc attatttatt ggccgggaac tggctgcgca ggtacaattg
    43501 gaaaaacctg tttctgctgg aatcgttgcc aatgaaattg cccggcccga attaaaatta
    43561 tatagtgagc agcaaaaatt cattggtcag caggaaaaac tgatcacggc taaaaattta
    43621 ccaagggcaa gtttgtttgc acagggtggt tatggccgtc cgggtttgaa tatgctgtta
    43681 aatgaatttg attttttcta catcggtggt ttacggctca actggtctct tggcggattg
    43741 tacacaaaga agaaagaaaa agaacaggtg actatcaata aaaaaatggt ggaagtgcag
    43801 aaagaaactt ttctgctcaa taccagctca cagttgaagc agcagcaggc agagattgac
    43861 aagctgcagc aactgctggt atcagacaat gagattatca gtttgcgaaa aagtgtaaca
    43921 gaagccgcca aagcacaact ggagaacgga gtgataacag ccaatgattt tttgaaagaa
    43981 gtaaatgcag aggaccaggc aaggcaaacg cttatcactc accaggtaca attattacaa
    44041 gcgcaaatca actatcaaac cattttaggt aaataataaa tatactatgc aacatatttt
    44101 taaattagcc ccgattgtac tgattctttt ccttagtgcc tgcaagagcg ggaacggaaa
    44161 ctttgatgca agtggcactt ttgaagcgga tgaagtgatc gtttcttcag aattgtcagg
    44221 taagatcata agcttttctg cggaagaagg acaatccatt ccaaaggata gtattgtggc
    44281 ggtagtggat gcagcaaata tttccctgca gaaggaacag gttgaagcca atattcaggc
    44341 attgagtgag aagacagctg atgttacacc gcaggtccgg ttactggaaa accagctggc
    44401 agtacagcaa tcattgctca ataacctgtt gcatgaacaa acaaggatac agaacctgct
    44461 gaagcaggat gctgccaccg gtaaacaact ggatgatatt atggcacagg tggatgtcgc
    44521 caaaaagagt cttgcgctta cgcagcaaca gatcaatgtg cagcgcagta atgtggcgac
    44581 acagaaccgt ggtatactca gtgaaggtaa accgcttgaa aaaagggtgg cacaattgga
    44641 tgatcaattg aaaagagcta cgattctaaa tcctgtcagt ggaactgtca ttaccaaata
    44701 tgcagagcag ggtgagatca ctgcagccgg taaagcttta tataaaatcg cagatctcag
    44761 tacaatgaca ttaagggctt atatcacggg cacgcaaatt tcgcaggtaa aactgggaca
    44821 gggggtaaaa gtcttcgttg atgatggtcc tgataaatac aaagaactgc cgggtatgat
    44881 cagctggatt gccgataagg ctgagttcac cccgaagacg atccagacaa aagatgaaag
    44941 ggcgaacctg gtgtatgcca ttaaagtgaa ggtaaagaac gatgggtatc tgaagatcgg
    45001 aatgtacgga gaggtaaaat ttagccaata gctgttagcc attagccatt aaaccaaaca
    45061 ctatatgaaa aatttcaagg agcttcttat ttggaaaaag ggaattgaag ttgtcaagct
    45121 ggtatataag ataacaaaaa tagttccggc acaggaaaaa tttggcatta tatctcaaat
    45181 gacccgtgca gctgtttcta ttgctgcaaa tattgctgag ggaagtagca gaaacagtga
    45241 taaagattat gccaggtttc ttcaaattgc tcttggttca gcttttgagt tgcaaactta
    45301 ccttgtcatt gtgaaagaaa tgaactgggc cgatgtgaat gaagttgcgc aggttgaatt
    45361 aatgcttgag gaggaaataa aaatgattca ttcttttatt aaaagactat cagctaaaag
    45421 ctaacagcca aatgccaaaa atctgtatat gtcagctgta atcgtaaaca atatcattaa
    45481 acggtatgga tctaaaaaag ctgtgatcac tgcgttgcag ggtgtcagct ttgatgtgaa
    45541 gcagggtgaa ctatttggaa tcatcggtcc cgatggtgca ggtaaaacat ctttgttcag
    45601 gatactgact acgctgttac tggcagatga aggaactgcc actgtggatg gatttgatgt
    45661 ggttaaagat tacaagtcga tccgtcagcg ggttggatat atgcccggaa aattttcgct
    45721 gtaccaggac ctgaccgtaa aagagaacct tgattttttt gctacaattt ttaatacgac
    45781 tgtagaagaa aattatgcct tgataaaaga tatttactct cagatagaac cctttaaaga
    45841 aaggaaagcc gggaagctct cgggtggtat gaaacaaaaa ctggcattaa gttgtgcatt
    45901 gatacatcgc ccgtccgttt tattcctgga tgaacccacc accggtgtgg atgccgtgtc
    45961 caggaaagag ttttgggaaa tgctgacaag gctgaaagaa cagggcatca ccataattgt
    46021 ttccacacct tacatggatg aagcaagtct atgtgacagg gtggccctga tccagtccgg
    46081 aaaaatatta tcaatcaata cgccccaggg tattgttgat tcatttggca agaaattact
    46141 ggccgttaaa agtaatgata tgctccacct gttaaaagac ctgaagcaat gtaaagaagt
    46201 ggaagatgct tatccattcg gtgaatatca tcatgttgta ctaaagaagg acggtgaaga
    46261 actattgaag agttacctgg agcaaaaagg gcatctattt aaattaaaag aagcgaagcc
    46321 caatattgag gattgcttta tggcgttaat gaaaacaagc taatagcaga ttgcaactat
    46381 gaatgaaaaa gtaattatag tagaaaatct gaccaagaag tttggcgact ttacagcagt
    46441 cgatcatatc agctttgatg tgcataaggg ggagatcttt ggtttccttg gtgccaatgg
    46501 tgccggtaag actactgcga tgcggatgtt gtcgggatta tcattgccta cttccggcca
    46561 tgcaagggta gcaggttttg atgtgtataa agaaaatgag aagatcaagc agaatatcgg
    46621 gtacatgagc cagaaatttt ctttgtatga ggacctgaca gtgaaggaga atatacgttt
    46681 ttatgcgggt atatatggca tgagtaaccg ctttatcagg gaaaaaacag ccatggtact
    46741 tgagaaactt catcttgctg ctgaaggtga taaactggta aagtcattac ctctcggctg
    46801 gaaacaaaag ctggcattct ccgtcgctat tttccatgaa ccacaaattg tttttttaga
    46861 tgaaccaacg ggtggtgttg atccggtaac aagaagagag ttctggcaga tgatctacga
    46921 ggcagccgaa tcgggaatta ctgtttttgt aaccacacac tatatggatg aggctgaata
    46981 ttgtaaccgg gtttctatca tggtggatgg caggatagat gcaatggata ctccacagtt
    47041 actgatagaa acgtatgatg ccatatcgat ggatgaagtg tttttaaaac tggccagaaa
    47101 agccacaagg gcagcagatt aaaaatgaaa caatttatag catttgtaaa aaaagaattt
    47161 catcacattt tccgggataa gcggacaatg tttatcctgc tgggtatgcc ggtggtacag
    47221 atcattattt ttggattcgc ccttactaat gaagtgaaga attcgagggt tgctattcta
    47281 gacccgtcaa aagacgctgc tactacttct ttgatagcac aaattgaagc cagccgttac
    47341 tttgaggtag atcagtatat tcattccacc aatgagattg agaccatatt tagaaaaggt
    47401 aagatcaaac tggccataat ttttccttcc catttcaggg aagatttgca acattttaat
    47461 aaagcacagg tacaattaat tgctgatgca tctgatccca atgtggcaaa tacattgacg
    47521 agctatgctt cggcgattat catggattac cagaacagga ttacgaatga ccggaaactt
    47581 ccctacacca tcaatacgga aatgcgaatg ttgtacaacc cggaattaaa gggtgcttat
    47641 aattttgttc cgggagttat ggcaatggta ttattgctgg tttgtactat gatgactgct
    47701 ataaccattg taaaggagaa agaaatggga acgatggaga tcatgctggt atctccaatg
    47761 aagccattga aggtagttct cgccaaagct gttccctatt tattgttatc atctgttaat
    47821 atagccagca tattattatt aagtgtattt gtactggagg tgcccattaa tggaagtcta
    47881 accctgctgg tagctgaaag tcttttgttt actctggtgt cactggcatt ggggttgcta
    47941 atttctacaa aagttgattc acagcaaact gctttattca tttcattagt cggcctttta
    48001 cttcccaccg tcatgcttag tggttttatg ttccccatag aaaatatgcc attgcctttg
    48061 caggttgttt ccaatgctgt acctgccaaa tggtattata ctattgtaag atcagtaatg
    48121 ataaagggaa ccggttttca ggctgtgtgg aaagaaacac tcattcttgc cggaatgctt
    48181 tttttctttt taggattggc aattaagaat tttaaaatcc gtttagcatg agaaccctta
    48241 aatttttatt acaaaaggaa ttcaggcaga tattcaggga tccctccatc cttcgcctga
    48301 tttttatcat gcccgtgatt caattaatta tgttgccatt cgcggcggat tatgagatca
    48361 agaatatcaa acttgcagtg attgaccatg atcattcctc ttattcacgc agccttgtta
    48421 ctaaaattac ttcatccggt tattttatac ttactgatta tactggttcc tttaaccagg
    48481 ctttcgagaa aattgaacag gataaagcgg atctaattct tgagatacct gcttcatttg
    48541 agaaggatct ggtgaaggag agtgagtcaa aaatatttat ggcggtaaat gctatcaatg
    48601 gcgtaaaggc cgggctcggc agttcctatc tgcggagtat tatccaggaa tacaacaaag
    48661 aagtaagaac agagtggatt caatttcccc ggtttaaccc ggagactaat attgaggtag
    48721 tgtattctaa ctggttcaat tcatccatga attataaggt cttcatggtt ccgggaatac
    48781 tggtattgtt attaacaatg gtgggcgcca atctcacagc cattaatatc gttaaggaaa
    48841 aagaaatagg tacgattgag caaataaatg taacgccggt ccggaagttc catttcattt
    48901 tgggcaaatt gattccattc tgggtgctgg gattattgat attcagcatc ggtttgggga
    48961 ttgcccggct tttttatggt atcattccgg caggcaatat actgaccata tatttgtttg
    49021 ccgctgttta tctttttgct gttttgggtt tagggctgtt gatatctact tatgcagata
    49081 accagcaaca agccatgctt atttcattct tcctgatgat gattttcatt ttattggggg
    49141 gcttatacac gtctattgac agtatgccgg gttgggcaca ggcaattaca aaagccaatc
    49201 ccgtttctta tttcattgaa gtgatcagga tggtagtgct aaaaggaagc agccttgcgg
    49261 atattaaata ccagttactg accattttgg gcttcgccat tgttttgaat ggctgggcgg
    49321 ttgtcaatta tcataagcgg tcctgataaa ttcccttttt aaaacagaca aactttttta
    49381 tattcggtta acaaaaaacg aattgccatg ctaaaaatca cacatctgaa agaaggcgac
    49441 attatcacag tagatgatga tggtataatg agagagggaa ctgtagtcag ggtaagccgc
    49501 gaagaacacc aggcactggt ggataacggg gtgcaggaat tctggtatag ccaggaagat
    49561 atgtattctt taccgctgga cgagggcaag ttgctcgagt ttggctttac ccgggaagaa
    49621 caggacggcg cggttaaata caaaaaggat tccttcaggc tggtaactcc ccaaaagggt
    49681 gattttacca accttgaaat gtggtggcgg gaagacaggc gtcatttcga ttttccgata
    49741 acagtacatg agcttcaaaa tctgtacctc gacatgacaa aagtgcacct cgacaaggct
    49801 tccaacgccg tctagggcac tgttttataa acaattaagt catttttcca cagaccccct
    49861 aaaattatcg ggggtttttt cttttttcgg gttgatacgc ttacctttgc gttcccaaaa
    49921 aattatgtcg aaacaaccgc tcattaaaca agatggtatc attctggaag ccttgtccaa
    49981 cgctatgttc agggtaaaac tggaaaatgg tcacgaaata ctggccacca tctctggaaa
    50041 aatgaggatg cattacatcc gaattctacc gggagataaa gttggagtcg aactttcgcc
    50101 atatgatttg acaaggggaa ggatcaattt caggtataaa taatgcttaa cgccaaaagc
    50161 tgaaagctaa aagtaaaaaa aatgaaagta agagcatcca ttaaaaagcg tagcgtcgac
    50221 tgcaaaattg tacgtcggaa gggtaagttg tatgtgatta acaaaaagaa tccccggttc
    50281 aagcaaagac agggttaatt aaagaaagca aaaaatcaaa aagcaagaac caaattgata
    50341 gttgagattt gtttcttgga acttaaatca aaataatatg gctcgtattg ccggtgttga
    50401 cttaccaaaa aataaaagag gcgaaatagg acttacctat atctacggaa taggtccttc
    50461 tactgctaag tacattcttg acaaacataa tattgacagg aataaaaaag tgcaagagtg
    50521 gaatgacgat gagttaactg atatccgtaa cactattacg gaagaattaa aagtagaagg
    50581 tcagttgcgt tctgaagtac aaacaagtat taagcgtttg cttgatattg cttgctaccg
    50641 tggattgcgt catcgcaaag gtttacctgt tcgtggacaa cgtaccaaga caaacagccg
    50701 tacaagaaaa ggtaagagga agacagttgc tggtaagaag aaggcagcta agaaataaaa
    50761 aataattagc tgattgttcg atgtactgat tagctaattt tttatacgaa gcatttcgaa
    50821 tcaattccag atctgcgctt cgcagggagg atagattcaa acccggttcg ccggaaacaa
    50881 acaaattttt aaagattacc taagaagaaa gattatggca aaagcacaac aacaaggtgg
    50941 tagcgcaaaa gcagcagcaa agaaaagagt agttaaagta gattcatacg gagatgctca
    51001 catttctgct acattcaaca acattattat cagtcttact aacaaaaccg gacaaacaat
    51061 tgcctggagt agtgctggta aaatgggttt cagaggttct aaaaagaata ctccttatgc
    51121 agctcagatg gcagcagctg atgcagctaa aactgctctt gatgctggtt taaaaagagt
    51181 tgatgtgttt gtaaagggac caggttctgg tcgtgaaggt gctatccgtt ctctttcaca
    51241 aagtggaatt gaaatagtga tgattaaaga cgtaactcca ttgccacaca atggttgccg
    51301 tcctccaaag aaaagaagag tataattttt atattatata gttataattg ttctgaaagg
    51361 atcagaacag ttcattttaa aaaagggtgc ctgattgatt ttttaacgct tttactgatc
    51421 agagcaccgt tacaaaaaag cgtaaatgaa taaaaactat ggcaaggtac actggtccaa
    51481 agaccaagat ttcccgcatt tttggagagc ctattctagg taatggtaaa tggttgggta
    51541 aaaacagcaa ccctcccggt cagcatggtg aaaaacgtaa gcgtaaaacc cttggtgagt
    51601 acgctttaca gctccgtgaa aaacaaaagg ccaaatacac atatggtgta ttagagaaac
    51661 aattccgtaa aacatttgag gaagcagccc gccgtaaagg tgttacgggt gaaaacctta
    51721 ttaaattact ggaagcccgt ttggataata ctgttttccg tatgggtatt gctccttcac
    51781 gtcctgctgc ccgccagctc gttagccaca aacacattga agttaatggg gaagtggtga
    51841 atgttccttc ttgtcaactg aaacccggtg atgttattac tatcaaagat ggtagcaaag
    51901 acagaacttc tatcaccagt gttgttcgtg ccaagaatcc gaaattcagc tggttggatt
    51961 ggaatgaaac cgagtttaaa ggtactttca ttacctatcc tgaaagggag agtgttcctg
    52021 aaaatattaa ggagcaactg atcgtcgagt tatatagcaa gtaatcactg tgaagccggg
    52081 acaacatccc gcaacttcag cttgctgatt acacaatcat ttaaaaccta aagtttacaa
    52141 tatggcaatt ttaaatttcc aaaaacctga taagatcatt ctgcaaaagg ctacggattt
    52201 tgaagctcaa tttgagtttc gtccgcttga gccgggctat ggtgtaacaa tcggtaatgc
    52261 acttcgccgt gtattattga attcactgga aggctttgca atcattggtg taaagatcga
    52321 aggtgtggag catgaatttg ctaccatgaa aggcatcagc gaagatgtgg tggaaatttt
    52381 cctgaacctg aagcaggttc gtttcaaaaa gacagttgat cacgaagtga caaatgagaa
    52441 aatcatgttg tctataaaaa acaggacaga attcacagcc ggtatgatcg gtgaaggtac
    52501 acaaaccttc caggtcatga acccggatct gctgatctgc acactggatt cttctgcccg
    52561 catggatatc gaactgacta tcggtaaagg ccgtggttat gttcctgcag aggataacaa
    52621 accaaaagat gcaccgctgg gttatattcc gattgatgcc atcttcacac ctatcaagaa
    52681 tgtgaagtac actatcgaga atacccgtgt ggagcaaaga acagactttg aaaaactaat
    52741 catggaagtt tctacagatg gtactatcca tccggaagag gccgtgaagc aggcatcaag
    52801 gatattgatc cagcacctga tgatcatcac agatgagaat atcactttcg acaataagga
    52861 agagaagaaa gaagacctgg tggacgaaca aactttacaa ttacgtaaag tattgaagac
    52921 tccgttggaa gacctggatc tatctgttcg tgccttcaat tgcctgaaag ctgcaaagat
    52981 caactcattg agtgagctgg tacaatatga gcaggaagac ctgatgaaat tccgcaactt
    53041 cggtcagaaa tcactggctg agatcgagca ggtattgaca gaaagaggac tgcatttcgg
    53101 aatggattta gtgaaactgg gaattgatct gagcgaattc taataccctc cgaatatctc
    53161 tgaagtgaga cccggaagga gtatttaaga ttgaatattc attattaatt aatgctgttt
    53221 taaaaaagca gcgaacattg caaatcctga cacggtgcaa tgtcttaaaa cttaaatgtc
    53281 atgcgtcacg gagacaaagt aaaaaactta ggcagaaaaa aagcacacag agatgcgttg
    53341 ctgagcaacc tggcctgcca gctgattcaa cacaaaagaa tcgttactac aacagctaaa
    53401 gccaaagctt taagagtgta tgtagagcct cttatcacta aagggaaaga caatacaacg
    53461 catcagcgtc gtgtggtttt cagctatctg caggataagg aagcagtaaa agaattgttt
    53521 gatacggtgg ctacaaaagt tgctggccgt cccggaggat atacccgtgt tattaagttg
    53581 ggtgcccgtc ccggtgataa tgctgaaaca gcaatgattg agctggtaga tttcaatgag
    53641 atctacggta aaggtaaagg tgaagcaaaa actgcagcta aaaagacccg ccgttccggt
    53701 ggtgcaaaaa agaaagcaga agctactgat aaagtggaag ctcctgcagc agaagagaaa
    53761 gcggcagaat aaacgaagcc aacatctgtt cgatagaatg cctccgactt taaaccgggg
    53821 gcattttttt attctatatt gtctgattac cctactgaat atctggggaa atgaagtatt
    53881 ttcatagtcc tgcttgctac ttcttttgaa tctttaaaac aacaaaatgg atcatcaatc
    53941 caacagggcc ttgcagcaat tcaggaatga aataggtatt aagttccagt tgtacaacag
    54001 tctttttaca tcattgcctt ttcatcggat agaaaaaaca ggtattcttt tatccctgtt
    54061 gtcaaacaat tgcgaggaag ggtataaaaa aagatcaagt ccctctgata ttattgagga
    54121 tttttttaat aaacatacta cttacgtaaa agaagatgaa aggaccgacc tgttgttccg
    54181 ctttgttcaa tatattgaaa ggcaggttgt attatttgat gcactggaag atgcagcttt
    54241 taaaagtgtg aatgatctga atggtgttgg tacactcaag catctggaat cggaagtgtt
    54301 gcaggggaac aaacaggatg aactggcagt taaattgaaa gattttgcta ttcggctggt
    54361 attaactgca cacccgacac aattttatcc cgggccggta ttgggtataa taaatgacct
    54421 ggccaaagca ttggctgaaa acaataccaa tcttgtcaat acttacctgc agcaattagg
    54481 taagactcct ttcttcaaaa agaaaaagcc tactccgtat gatgaagctg taagcctgat
    54541 ctggtacctg gagaatgttt tctacccggc agctggcaga attatttcat cattgaaaaa
    54601 ccagtttcct gatgcagtaa gtaaaaataa tcctgtaatc cgcatgggat tctggcccgg
    54661 gggcgacagg gatgggaatc cttttgtaac aagtgatatt acattgcagg tgtccaatgc
    54721 cttgcgcagc agtattgtga agtgttatta tatggatgtg aggagactga gaagaagatt
    54781 gacttttgca ggtgtggaag tgtttttaca ggaactggaa aataaattgt acaacaatct
    54841 ttttattccc gggtttaaaa ccaatctcaa aaaggatgaa gtactcataa cccttaatca
    54901 gataaaagag gtgatcattc atcagcataa tggattgttc ctgcatatgg tgaataacct
    54961 tatcaataaa attgaggtat ttggattaca ctttgcatca ttggatatcc gccaggacag
    55021 ttccgtgcat ggcaaagtgt tacaggatat tgcagctgat cagcagttat tacctgcagg
    55081 gtatgcaggt cttacagaac aggagaagat tgaaatctta tcttccctca cagggattgc
    55141 ggatgctgac tcattatctg atcctctgtc acaggataca ctgaaaacta tccgggttgt
    55201 taaagaaatt cagcaattca acggaacgga aggctgcagc cgctatatta tcagtcagtg
    55261 taacagtgct ttaaatgcga tggaggtata tggacttttt ttgttagcag ggtggaagaa
    55321 ggaagaaatg gctattgata tagtcccatt gttcgaaaca attgatgatt tacaggaggc
    55381 ggctactgtg atgcgtactt tatatacaca tcctgtttat aaagaacact tgaagcgaag
    55441 aggaaatcag caaaccatca tgcttggttt ttctgatggg acgaaagatg gcgggtacct
    55501 gatggccaac tggagtattt ataaagcaaa agaggagctg acgaggattt ccagggaaaa
    55561 tgaagtagat gtgatatttt ttgatggcag gggaggtcct ccttccaggg gtggaggaaa
    55621 gacacataag ttctatgctt ctatggggca taatatttct aataaagaaa ttcaattgac
    55681 gatccagggg caaactgtaa gctcgaattt tgggactatt gatgcagctc agttcaatat
    55741 tgagcaatta ttgaatgccg gaatttcgaa tgatcttttt tctaaccgtg atataacgtt
    55801 gaatgagaat gaggaaagcc tgttacagga attggctgaa gcaggattta ctgcgtacaa
    55861 atcattaaag gatcacccat acctggctga ctatttatta caggtcagtc cactgcgatt
    55921 ttacagcgaa acgaatatcg gtagccgccc ggcaaaacgg ggtgcttcat caggattatc
    55981 tttgaaagac ctgcgggcaa ttccttttgc cggctcctgg agccagttga agcaaaatgt
    56041 taccgggtat tatggcgtgg ggtctgcatt acagcaaatg gaaaagcagg ggaaattatt
    56101 gcagttgcag aaactgtatc atcagtcatt atttttccgg accttactgg ataactgtga
    56161 gatggcaatg atgaaatcct tttttccact taccgctttt ctttcaaacc acaagcagtt
    56221 tggcgaatta tggcatatga tctatgatga gtacgaactg accaggcaat atttgttcaa
    56281 gataacagga cataatgaac tgatggctga ttatcctgtg gaacaattat ctatccagat
    56341 gcgggaaaga attgtattgc cgctggtaac gattcagcag tatgccttaa ctaaaataag
    56401 agaactggag gaacagttag caccggcaca tacaaaagag acctttgaaa agctggcgat
    56461 gcgatgttcg ttcgggatca taaatgcggg taggaattcg gcgtaataat ttaccaatga
    56521 ggatatcaat ctactatttt tgcatctcaa tttactgata tgaataaggt aaaagttgga
    56581 ttggtgcaga tgagttgcac caacaagaaa gatcagaatc ttaacaaagc cattgagggg
    56641 gtaaggaaag ccgctgctgg aggtgcacag attgtttgcc tgcaggaact cttcacctca
    56701 ttgtatttct gcgatgtgga agattatgag aacttcaaac tggcagagcc tgttcccgga
    56761 ccttctacag aagcactgca gaaagtagct ggtgagttag gagtagttat catagcttcc
    56821 ttattcgaaa aaagggctca gggattatat cataatacaa ctgcggtgat agacgctgat
    56881 ggaagttatt tgggtaagta tcgtaagatg catattccgg atgatccggc ttactatgag
    56941 aaattttatt ttactcccgg tgatctgggt tataaagttt ggaaaacaaa atttgggact
    57001 attggtgtat tgatctgctg ggatcaatgg tatccggaag cggcaaggat cactgcattg
    57061 atgggggcag aaattttatt ctatccaacc gctatcggtt gggccaccgc ccaggacgaa
    57121 gcaactaata cggaacagta tggtgcatgg caaacgattc agcgaagtca tgctatagca
    57181 aacggagtgc atgtagtaag tgtgaaccgg gtaggacttg aacagaatgg tgcgatgaaa
    57241 ttctggggcg gttcatttgt ttccaatcct ttcgggacta ttttgtataa agcttcacac
    57301 gaagaggaag aggtaaaagt gcttgagctg gacctggcaa aaactgatca atacaggact
    57361 cactggccat ttatgagaga tagacgaatt gattcttacc aaccaattac caaacgattt
    57421 attgacgaag agtagagaga agtccgtaag atggaaagac agtaagtccg aaagttagtt
    57481 tcctttaatt gaaattacac gattaaatag ttgattatgg cttttaagtt tgaaaagctg
    57541 attgtatggc aaaaggcagt tgacctttct gatgttgtta atcaggttac aaaacaattt
    57601 ccaaaagacg aagtttatat tttgacatcg caaattaaaa gagcggccga ttcagtttcc
    57661 ctgaatattg cagaaggttc aacaggtcaa tcaagcccag agtttaatcg atttcttggt
    57721 attgccctgc ggtcggccat tgaagttgtt ggttgtattt tcttagcaaa gagacgagaa
    57781 tatatttctg atgatgattt tgataagatt tataagatgt gcgaagaaat attggttatg
    57841 attaatgctt tacgcaattc gttaaaataa aactgtaata agttgagtac gcaaaaacat
    57901 tccgtctttc cgtcttccgg actttcagac tttcctaaaa attcaggtta ctattttccc
    57961 gccgaatttg ctccacatgt tgccacctgg ctgagctggc cgcataagga agcctcatgg
    58021 ccaggtaaaa tccatactat atatccgtct tatgcaaagt tcattaaaga attgacaaag
    58081 ggggagcttg tccggatcaa tgttgctgat gaagctatga agtcatttgc cattggccat
    58141 ttgcaaaagg aagaagttga cctgagcaaa gtcgaatttt tctttcatcc tactaatgat
    58201 gcctggtgca gggatcatgg tccggcattt ttaattaacc cggatgcaaa gattaaaaaa
    58261 gttattgttg actggggtta taatgcctgg ggaaacaaat atccgccgtt cgatctggac
    58321 gatgtagtgc caacattgat cggtaaacat tttaatattc cggtttatca tccgggtatt
    58381 gtaatggaag ggggctctgt tgagtttaat ggaatgggaa ctgtaatgac ttctacagca
    58441 tgtttactga accccaaccg aaatccacat ttgattcagc aggaaatcga agagtatctc
    58501 tgtaattatt acggaatgga gcaggtattg tgggtggatg aagggattgt aggagatgat
    58561 acagatggtc atattgatga tacagtacgc tttgttaatg aagacacggt aataacggtt
    58621 attgaagagg ataagaatga cgagaattat acgttacttc aacataatct caggcagctg
    58681 aaagaaatgc gtttgattaa cggcaagcaa ctcaatattg tggaattgct gatgccggag
    58741 gaattgattt atgaagatca acgtcttcct tgctcttatg ccaattttta tattgctaat
    58801 aaatcagtta ttgttccaac ctttcgttct gcaagagacg aaaaagccct tcggataatc
    58861 caggattgct ttccgtcaag agaagtggtg gggatagatt ctactgatat tatctgggga
    58921 ctgggtagtt tccattgtct tagccagcag gagcccttgg tataaaaaag ccttctgtag
    58981 aaacagaagg ccgctaacga ttgcttgcca catagagtct ttataatctt ttctcagaaa
    59041 gaatagattg ctgcgatcag aaaattggca gctgtctttt tcgtaactcc atccttatca
    59101 ataaatattt ccttgtttga attgtcaagg cggaattcgg gaataaaagt gaaacctccg
    59161 gattttaaat tcgctgaaaa tgttgttgcg aaaatattac taccatcagg ggatgccatt
    59221 attttcaggt gattcttatc gccgaaatat tctcccctta gtgtgagacc aaaccagggc
    59281 tgtggatcgt aattcaggta taatgctgaa ccccaccagg attgactctc gatatttttt
    59341 gcaccatccc aaaaccgggt agaattaatg gtgccattat atcctatatt gaatttgtca
    59401 ctgatttttg cagtgataac gccatcgaac tggttcactt ttgaagtatc cgggttttgt
    59461 ccacccacgt agttcaggta aatcttaatg ttctctgtag gagcatagga gtattgggcc
    59521 aacaagaatt ttttattgat ctgcccatcc attggaattc tgaaatcggt agcattagct
    59581 attcccagca tagcaccatg ttttcccatt gatacttcag cttttaaccc ggtatgagag
    59641 aaaggaccat tcgtgaacat atagctcatg ctatagtttc tgttcagttg cggatctagt
    59701 aattcatacc ccacatgagt gccccatgtt cctgctacga attttaacca gtcagcagga
    59761 gagtagctta catacaattg tttaatggct tgtgtgatgc cgttatctgt gtaggaaaaa
    59821 tcttttgccc tggggccaaa tccaagatca gccactgcac ctactttatc tcctttgtgt
    59881 tcaaatttta cactggccat ccccagggaa aaggcattat gagtttgtgt aaagctggtc
    59941 aggctattga cttttgtatt tgcaaagtca agtttataat aggcatcaac ggagccggtg
    60001 attgttaacg acgcttcctt tctttccccg gcagcaacag tagctgtgga atccgctgtg
    60061 ttattgttta ttgatgctgt ttgggctatc gagagggtaa atgacagcat actgatggct
    60121 gccacaaaaa gtttttgtaa cattgtatta cattttagat gttaagaaaa gggcacagca
    60181 ggaataagct tttgcgagaa gtttatccca cttatgcagt gcctttttcg attttatttg
    60241 cttagtgttc tacaggtttt tcttctatag aaccattgtt gtgaaccagt aatgttcctt
    60301 gcaggtattt ttcatcatgc tgggtagcat ccagtccttc ctcttcttca ctttcactta
    60361 ctctcatggg aattacaaaa ttgatgaact tgaatattcc gaatgaaacg gtaaagctat
    60421 aggccacaac gatggacatc gctttcaact gcgtgaagaa aaattcagga ttgccataaa
    60481 ataaaccttg tggtccgtct ttgatgccat gtactgcagt gctggcaaaa acacctgtaa
    60541 gtaacatgcc aaccatgcca cctaatccgt gacaagggaa tacgtccagt gtatcatcca
    60601 gttttgattt ctgtttaata ctaactgcaa tattggaaac caatgctcca accaggccaa
    60661 tgatgatgct ttgtggtatt cccacaaagc ctgcagcagg tgtaattgca acaaggccaa
    60721 ctactgcgcc aatacagaaa cccagtacag aaggtttttt gcctttaata acatcaaaga
    60781 acatccaggc caaacctgcg gaggaagcgg ctgtagtggt agttccaaaa gcatttacag
    60841 ccaggggggt ggcgcccact gcagaacctg cgttaaaacc aaaccaacca aaccaaagta
    60901 aacccgtgcc gatcaataca tatggaacat tggcaggagg gatctcttta ttctcaatat
    60961 gagactttct gcgttttagc accagggcac cggctaatgc ggcacaacct gccgtgatat
    61021 gaacaaccgt tcctccggca aaatcccatg cacccatttt tgccagaaat ccttccggat
    61081 gccagctcca gtgtgcaaca ggtgcataca ccagtagtgc aaaaagcaca ataaacagta
    61141 catacgaagt aaaacggatt cgttctgcaa ctgctcccac taccagtccc ggtgtgatta
    61201 tagcgaacat cagctggaat agggagaata atgataacgg tatagtgttg gccaatggcc
    61261 aggcagctcc tgactttacg ccattataaa atgcgtaagt ggagggattg ccaatgaaac
    61321 cgttaattga ttctccgaaa gcaagactga atccgcaaat cacccaaagc accccaacaa
    61381 taccggccgc aacgacactt ttaatcattg ttgagattac atttttccgg tttaccattc
    61441 cgccatagaa aaaggcgaga cccggagtca tcaggaatac cagtgctgta gacactaaca
    61501 cccaggcgat atcagaagag ctatactggg gatcagcctc acccggaaca taatcagctg
    61561 cgggttttac aaacatggca ataatggcaa tcgcaaccaa cagtacaaat ggcaagattt
    61621 gttttgcttt tcttttactc ataagctggg ttttaattaa acgttaaaaa aataaatcat
    61681 ttgatatatc tggtataaaa atatcaaaat atggcattaa catatgttag tatatcatat
    61741 tatttcaaat tattattgaa atagttgata aattgtttaa aatattaaat attaataaaa
    61801 aataatattt aatatttagg aatttaattt gagtctataa agcctgaaat tgaaggatat
    61861 tgtccctgga ttctaacatg ctgggagcat tattattcag aaactcatcc actttacggg
    61921 cacattcccg tccttcgctg atggcccata ccaccaggct ttggccacgg cgcatatcgc
    61981 cggctgagaa gatcttggag atattagtac gatagtcttt ttcagatgct tttacatttc
    62041 ctctttcatc gagccctata ccgagatcgc tgataaagcc tacatgttgc ggatgcacaa
    62101 aacccatggc taataatgcc agctcacaag gaatttcttt ttcactacca gccacttcaa
    62161 caaattgtgc gggtctacca tctacaattt tccattcgag gtcaataatt ttcaatgctt
    62221 tcagattacc tttttcatca ccaataaatt ctttggttgc aacggcccac tgacgtttag
    62281 caccttcttc atgcgaagta gttgtcttca gcagcatcgg gtaagtgggc catggcatgt
    62341 aattatttct ttcttccggg ggtttaggca gtaattcgaa ttgagttact gattttgcac
    62401 cctggcggtt agatgttccc acacaatcac taccggtatc gccgccacct atcaccacta
    62461 catttttgcc tgttgctttc aattcttcac ttaaaatgtt actttctatt gctgcgttag
    62521 ctaaaggatc ttttccggct actcttttat tattctgctt taaaaaatcc atagcaaaat
    62581 gaactccgtt gaattctctg ccgggaataa ataaattcct tgctgctgta ctaccacctg
    62641 ccagcacaat tgcattgaac tctctcagta tatcattgat actgacattc actcccacat
    62701 ttgccaggca tttaaaaata ataccttcct cttccattag ttttattctt ctgtcgatca
    62761 cccatttttc caatttgaaa tcagggatac catatcgcaa caatccaccc ggagcatcat
    62821 ctctttcgaa aacagtaact gaatgcccgg catagtttaa ttgtgcagca gcagctaatc
    62881 cggctgggcc ggaaccaatc actgctactt tttttccgga acgcaggtta ggtttttttg
    62941 gtaaaacaaa acctttttca aaagcaatct caatgatatg tttttctatt tcctcaatag
    63001 taatggctgg ttgattaata cccagcacac aggcagtttc acaaggggcc gggcaaattc
    63061 gtccggtgaa ttcgggaaaa ttattagttg atgaaagaat agcataggct tctttccaat
    63121 cttttttata aacggcatca ttgaattcag gtatcacatt tcctaacggg cagccatgat
    63181 gacaaaaagg aacgccgcag ttcatacaac gggccgcctg ctgattcagt tgttcatcag
    63241 tatagcggtt tacgaattca ttgtagtgct gtactctttc ctgtaccggt tgtttgccgg
    63301 ggagttctct gccaaattcc ttaaatcctg ttgggttgcc catagtatag ctcaggtatt
    63361 aatcagttaa ttatactttt atttttcttt aagcattttg ttttatgcct tcagctttct
    63421 gtaataacac ttttttataa tctcttggga acacttttac aaaatgctgt aattggttat
    63481 ctaaatcttc taaaatgaat tttgctacgg tactgccagt atattcaaaa tgtttctgaa
    63541 tcatggtaaa caattcattt ttatcattca ggtcacaagt atcaaggtcc accatctctt
    63601 tattgcagtt atcagagaac ttccctttta catcatatac atatgcaata ccaccgctca
    63661 tgccggctgc gaaatttctt ccggtatcac ctattattac tactctcccg ccagtcatgt
    63721 attcgcaacc gtgatcgcca accgattcca ctaccacgtt ggcgccggag tttctaacac
    63781 caaatcgttc gccggcctta cctcttatgt aggcctctcc ggatgtagct ccgtataaag
    63841 ccacattgcc aattatgctg ttatcttccg gagaataagt ggctgttttt ggtggataga
    63901 taataagttt tgctccactt aatccttttc cgaaatagtc attggcatca ccttccagtt
    63961 ccagtgtaat tccatttgta ttaaaggcac caaaactttg cccggcagtt ccgttaaaag
    64021 tgaaatgaat cgtattgtct ggtaagcctg ctgccctgta ttttttggtt atttcatttg
    64081 acaaaatagt gccaacggtc cggtcggtat ttttaatatt gaacagagca cttacttttt
    64141 gtgtgctctc aatagccggt gcagccgcct gtaataattt ccagtcgaga gtatctgcta
    64201 atccatggtc ttgctcttca ctgcaaaaca atgttgtgta cattgagtta ggttctttat
    64261 atagcaccga agagagatca aggtttttat atttccagtg ttcaatgttc tctctcattt
    64321 caagattatc aacctgccct atcatttcat tcactgttct gaaaccaagt tctgccatta
    64381 tttctctcaa ttcctgtgtg atcattttga agaagttcac tacatggtca gggtcacctt
    64441 taaatctttt tctgagtccg gggtcttgtg tagccacgcc aacagggcag gtattcaaat
    64501 ggcactttcg catcatgata cacccttcca ctaccagcgc agcggtggca atgccccatt
    64561 cttctgcacc taatagggca gccacggcaa tatccctgcc tgttttcatt tgtccatctg
    64621 cctgcactac tacccggctt ctcaacttat tttttaccaa tgtctgctgt gtttctgcca
    64681 atcctaattc ccatggaagg ccggcatgtt taatagaact caccggtgaa gcaccagttc
    64741 ctccatcatg tcctgatatc aacaccacat ccgcctttgc ttttgtaact ccggctgcaa
    64801 tggttcctac tccggcttta cttaccagtt tcacacttat tcttgctttc cggttggcat
    64861 tcttcaagtc ataaatcaat tgtgccaggt cttcgatcga ataaatgtca tgatggggtg
    64921 gaggagagat caagccaacc ccaggtgtgg catgacggac cttaccaatc caatcatcta
    64981 ctttatgacc aggtaactga ccgccttcac ccggcttggc accctgcgcc atttttattt
    65041 gtagttcatc tgcttccgtc aggtataaac ttgttactcc aaatcttgca gatgcgacct
    65101 gtttgatcgc acttctcata gaatccccat ttcccattac ttcatacctt ctttcatctt
    65161 cacctccttc accagtattg cttttagcgc cgagacgatt catggcaatt gccagggtag
    65221 tatgtgcttc ccatgaaata gagccaaagc tcattgcacc cgtagcaaat cgtttataga
    65281 tattctctgc tgcttccact tcatcaatac ttacagaagg acggttcttt ctgaactgga
    65341 acatacttcg gagtgtgcag gctttttcgc cctggtcgtt caccagttta gaatattttt
    65401 tgaatgtatt gaagtcattc atcttcgttg catgctgtaa caggtgaatg gtttgaggat
    65461 tgaataaatg aaattcccct tttcttttcc attgataaat accacctgcc ggtaacctgt
    65521 ctacaggaat atctttttta ttaaaagcaa agaaatgttt ggccaatgct tccctggcga
    65581 tttcatccaa tcccatacct tcaatccttg atgtagcacc ggtgaaatat ttattgacta
    65641 cttctttatt taatcccagt atctcgaata tctgagctcc ctggtatgac tgaagggtag
    65701 agatacccat tttggaaaat actttcagta aaccatcgtt gacagctttg atataatttt
    65761 tcttcagttc atcctgtgta agtgtagttt gtaattttcc ggactccctc atatcacgaa
    65821 tagaagaaag ggccaggtaa ggattaacag ctgttgcacc aaatccaacg agacaggcaa
    65881 aatgatggac ttcccaaaca tcaccggctt caacgataat gccgacctgt ccccgcatac
    65941 ctttacgaat aaggtgatga tgaatagcag ctgtagataa tagtgacggg atgggggcat
    66001 gggtagagtc tatagcccta tcctgtaata tcaatacttt aaaaccatct tctacggcgt
    66061 gaacagcgta ttcacaaatt ctgtccaacc ctttctgtag tgatcctggt ttgccatctg
    66121 ctttaaaata acattgaatt gtttttgatt gaaataaccc tgtatcaata ctcctgattt
    66181 tttccaattc atagttacta agtaccgggt gcttcaaagc cacgctatga caagtcagcg
    66241 ggtcttcaag caggagattt ccattattgc cggcgaatgt tgctaaagac attaccagtc
    66301 tttcacgaat tggatcaata ggcggattgg ttacctgtgc aaataattgt ttgaaataac
    66361 tgctgaggtg ttgtggctgg tcacttaata ttgccagcgg cacatcagtg cccatcgaac
    66421 cgatcggttc ttttccttct actgccattg gtgcaataat ggtatccagg tcttccgttg
    66481 agtacccaaa tgctttctgg tatttaaata cctggtcgtg ttcaaggtgg gtgaacataa
    66541 ttcttggttc gggtaattct tccaacctga ttttatattt attcaaccat tcaccataag
    66601 gcttttgaga acaaatttct ttttttaatt cagtatcact gatgattttc cctttctcca
    66661 tatccaccac aaacatctta cccggttgca atcttccttt ttcaattata ctggccgggt
    66721 caaccggcag tacacctgtt tcagatgcca tgatcacacg gtcatcagta gtgacgcaat
    66781 acctggatgg ccgtaacccg tttctgtcta atgtggcacc aataattttc ccatcagtaa
    66841 atgaaatgga cgcaggccca tcccaaggtt ccataaagga agaatgaaat tcataaaatg
    66901 cttttttcac cggatccatt tcctcatgtc catcccatgc ttcaggtatc agcatcatca
    66961 ttacatgcgg taatgaacgg ccggtcaggg tgagcaattc aatgatatta tcgaggcagg
    67021 ctgaatcgct ttgcccttcc gtaacaatgg gcaacagcat ctccatttct tcttttgaaa
    67081 aataaggagt ggtaaagcct ttttcgctgg tcttcagcca gtttaaattt ccctgcagtg
    67141 tatttatctc accgttatgg gcaatgaaac gaaatggttg tgccagtttc cacgatggaa
    67201 aagtgttggt agcaaatctt gaatgcacca ggccaaatgc actcactact cttttattac
    67261 tcaggtctgg aaagtattgc ctgacctggt gactggtgag ctgtccctta taaacaattg
    67321 ttttgtaaga taaggaggcc atataaaatc cgatatcatc ttttctgacc gtattgttga
    67381 taagatgaga ggcgtagttg cgcaatacaa ataattttct ttcaaattct tcagaatttg
    67441 aaatatgatc cgggcaggca acaaatacct gctccatctc aggctctacg cttaatgcag
    67501 tggggccaat atcagcagta ttcaccggaa cttttctata ggctaaaatt tccaatccta
    67561 atttctcagc tgttcggttc aaaatatcac ggcactcttc ccttaaacgg atttctctag
    67621 gaaagaaaac cataccagcc gcatattttc caaacgaagg gagatgaaca cctaatcgca
    67681 ggcattcgtc aaagaaaaat tcatgaggag tctgaatcat gatgcctgca ccatccccgg
    67741 tatttttttc acagccacag gcaccgcggt gctccatatt ttctaatact gttaatgcat
    67801 cggaaatgtg ctgatgggac ttatgtcctt tgatattggc tacaaaacca ataccgcatg
    67861 catcatgttc gaaggatgga tcgtatagtc cctggttgcc tgccatgtgg tgcaatgttt
    67921 agtttaatgt atacagtcgt tacctatatt tcaacagcaa gcaatacgtc tgaaattaca
    67981 aaaaagcttt cagcaggaga cgggattata agtgtactta tccacaattt gatcgggatt
    68041 aatctattac accaggtatc cgaacaactg gtcgttttta ttaatctcag aaaaaccaat
    68101 attggaatct tgcattttct tcaacaacaa ttcataatca gaacggcttt gtagttcaac
    68161 accaactaag gctggcccgt tctctttatt tgtcttttgc atgtattcaa accgggtgat
    68221 atcatctgtc ggaccaagta cattattaac aaattctctt aatgcacccg gacgttgggc
    68281 aaagctgatc aggaaataat gtttcagtcc ctcatattgc aggcttcttt cttttatttc
    68341 ctgcatcctg tctatgtcat tattactgcc gcttacaata catactatat tctttccctt
    68401 tattttatca gcataatcat ctaatgctgc aattgataat gcacccgccg gttccactac
    68461 aatggcatct tcattataca atttgaggat agttgaacaa acttttcctt ccggtaccag
    68521 gtgcatacca tccaatacat ctttgcaaat agtaaatgta agatcaccaa ctcttttgac
    68581 tgcagcacca tcaacaaatc gttcaatgct gtctaatgta actgggtggc ctgcttttag
    68641 tgcttcgaac atggaaggag ctccttccgg ttcaaggcca ataacctttg ttttgggaga
    68701 gtaagttttg aagtatgcgc caacaccggc actcaggcca ccaccaccaa ctggtacaaa
    68761 taaataatca acatcaggct ggtcctccag tatttcaact gcaactgttc cctgtccttc
    68821 gatgatgcgg taatcatcga agggggggat aaaagtcata tcattttctt ctgtgaattg
    68881 tttggcagca acagcacaat catcgaaggt atcacctatg agttttatct cgatataatt
    68941 ctccccgaac atcttcgtct gattcacttt ctgtttggga gtaataatag gcatgaatac
    69001 cacacccttt gcattcagtt ttttgcagct gtaggcaaag ccctgtgcat ggttgccggc
    69061 actggcacaa acaactcctt tctgcaactg ttcaaccggc aacaaactca tcatattata
    69121 ggcgcctctc aatttgtaag aacgaaccac ttgcaggtct tcccttttca gaaacacatg
    69181 acactggtat ttcttcgaca ggctatgatt atatgtaagt ggtgttctgt taaccacttt
    69241 ttttaatcga agatgtgctg ataaaaaatc gagctgcacc gtattgttaa ttgtttgtgc
    69301 catatactat tattagccta ccgactctgg tttttgtgcc agtttactga atacttcttt
    69361 tgttgatgat gcagtggcag ctgtagttgt tacagttttt gactgattct ctggtctcag
    69421 gctcctgact gttttacctg cctgccacaa ttcactttca cgcagttcct ttaactcttc
    69481 ttccagtttt tccctgtaat ctgctttact gttactctcg atagaacgct gcgattcttt
    69541 accggtagca acgctgtcat acaattcatt gaatactggt gctgttgcat cacggaattt
    69601 tttccaccaa tccaatgcac cacgctgcgc tgtagtagag cagttcgcat acatccagtc
    69661 catgccgttt tctgctacca atggcatcag tgattgtgtc aattcttcca ctgtttcatt
    69721 gaatgcttct gaaggagaat gacctctttc ccgcaacacc tggtattgtg cagcgaagat
    69781 gccttgtatc gcccccatca gtgtacccct ttcaccggtc aggtcactat atacttcttt
    69841 cttaaagtct gtttcaaata aataaccgct tcccactgct attcctaacg caatgactct
    69901 gtcaaatgct ttgccggtag catcctggaa aatagcatag gagctattca gtccacgtcc
    69961 ttgtaagaac attcttctta aggaagtacc cgaacctttc ggagctacga ggaatacatc
    70021 cacatctttt ggtggaataa tgcctgtttg ttcattgaat gtaatgccaa agccatgaga
    70081 gaagtataag gctttgcctg gtgttaaatg tttttgaaca gtgggccata aagcgatttg
    70141 tgccgcatca cttagcaggt aacaaatgat cgtacctctt tgtaaagctt cttcaatttc
    70201 aaataatgtt tcacccggaa caaatccgtc acggacagct ttatcccagg tttttgaatt
    70261 tttacgctgg ccaacaatta cattaatgcc attgtctttc tggtttagtg cctggcctgg
    70321 tccctgcacg ccatacccga taacagctac tgtttcattt ttcaatactt gctgggcctt
    70381 actcaacggg aattcttccc tggttacaac gttttcttca acgccgccga aatttaattt
    70441 tgccatgatt atctgttttg tggttgttat ttattgattg ttgattgttt acatggtaaa
    70501 tacctcttcg tttttatcga ggtattcatt ttctatcact tcttctcccg gttctttttt
    70561 ctcaaactct tttaaccggt catggaagct gttgctggct ttaatgatag caactcttgc
    70621 actgcggaca aattcaatca atccataggg ctccagcgct tttatcaggt tatccgtttc
    70681 ttcccggtga ccggttgttt caaaaaccgt atagtcttta cggatggcca cagcccttgc
    70741 accatattct ctcagtaacc tctctacttt taccttttca gtaatggcat cagtacttac
    70801 tttatacagc gccagttcct gccagacaat ttcttcttcg gtgttataat aagcttttaa
    70861 tacttcaacc tgcttttcaa tctgccggca tagttttttt accacttctt caatttcatt
    70921 gatgacgatt gtaaagcgat gt
//
LOCUS       DAORTI010000003        70231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_172_length_70231_cov_8.347669, whole genome shotgun sequence.
ACCESSION   DAORTI010000003 DAORTI010000000
VERSION     DAORTI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 70231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70231
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_70231_cov_8.347669"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1644)
                     /locus_tag="PLO70_00885"
     CDS             complement(196..1644)
                     /locus_tag="PLO70_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQZ73035.1"
                     /translation="MFKKLDKLIVKAFIGPFIATFFITLLVLVMQFFWLYIDDFVGKG
                     LGTNVILEFILYQSAVLVPLALPLAILLSSLMTFGNLGESFELVAIKSAGISLLRFMR
                     PLFFVSLFISGIAFVFSNYVIPVANLKSRTLLADIVYAKPAFDLKEGIFYDKISGFAI
                     KIGKKEANDSVIRDVIVYEQGNPLQDNFIVAKSGIMRVTENKRFLEFNLKDGWRYQER
                     GNVYEKTNTEYVRIGFKEYKKQFDLSNLGFTNRTADSVNKNNERMFSMRQLDKAIDSL
                     KKENGRIREQTTKDMFALLQFSALTDSSWKLPETADSSIVKEVKSFDDILPDSARINI
                     NQMAKSTAGSVRISAESLTQTMKDKEKNLRKHKIEWHRKIALSMACLVLFLIGAPLGS
                     IIRKGGLGTPLIFAIIFFMLFYFTSTTGEKFAKENTLTPFAGMWMATFVLVPVGLFLN
                     YKAMRDSQLFNKDAYKRIARVIGQFFQRRAGK"
     gene            1658..2269
                     /locus_tag="PLO70_00890"
     CDS             1658..2269
                     /locus_tag="PLO70_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73036.1"
                     /translation="MNAAKIRLSPEERELVMNAGWILTKNNILQKINRQLGELQSAQQ
                     EYLQNFSQSLPAAIMNSSPKISKGENYKGLPYLILDYPKYFDQSAVFAIRTMFWWGNF
                     FSTTLHLAGTFKDEFQEKIIAAFPLWQENGFCCCVHQEQWEHHFEPGNYKPVAELSKT
                     EFMNQIRNKSFCKLAKNYPLDNWDELPGLLLKDYMLLVTITGK"
     gene            complement(2272..2649)
                     /locus_tag="PLO70_00895"
     CDS             complement(2272..2649)
                     /locus_tag="PLO70_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HQZ73037.1"
                     /translation="MSKQLYTLEFPVRCSPSILYEFLSTPAGLGEWFADKVDERDGIF
                     FFGWNGSFEKADVLEKEQDKFIRFHWLTAPKEEYFEFRIDKSEITNQTILIIKDFAEK
                     KEIKDQTMLWDYQVKELFHRLGN"
     gene            2958..3827
                     /locus_tag="PLO70_00900"
     CDS             2958..3827
                     /locus_tag="PLO70_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQZ73038.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELITPEEEVKLATLIK
                     QGCQKSLDRLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGLTNRIQKAFSLLEQQYEREPSAEELAE
                     LLEMDIEEVTSTLGINGRHVSMDSPLSEGEENTLIDVLENPNAERTDGELDHTQSLKT
                     EIDRSLKALTERQKEVICFFFGIGVDHPMSLEDIGERFNLTRERVRQIKDKAITKLKT
                     SNRCKLLRTYLGA"
     gene            3866..5278
                     /gene="ffh"
                     /locus_tag="PLO70_00905"
     CDS             3866..5278
                     /gene="ffh"
                     /locus_tag="PLO70_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQZ73039.1"
                     /translation="MNIRCSLFLLLTMSPGAGQREANENESKQMFNSLQEKLESAFKN
                     LKGQGRITELNIATTVKEIRRALVDADVNYKIAKEFTDKVKDKAIGEKVINAISPGQL
                     MTKIVKDELAELMGGQEASFNAKGNPAVILIAGLQGSGKTTFSGKLANFLKTKKGLSP
                     MLVAADIYRPAAMEQLRVLGEQIGVDVHIELENKDAVDIARNAVKEARSKNKNVVIID
                     TAGRLAVDEVMMAEVANVKNAVNPQEILFVVDSMTGQDAVNTAKAFNDRLDFTGVVLT
                     KLDGDTRGGAAISIKYTVNKPIKFTSSGEKMDTLDVFYPDRMAQRILGMGDITSLVEK
                     AQEQFDEVEAKKLEAKLRKNKFDFADFKMQLEQIKKMGNMKDMLSMIPGMGSKIKDLD
                     INDDSFKGIEAMINSMTPEERADPDLIDGSRKKRIAKGAGKDILEVNNFMKQFEQMRG
                     MMKNMNKMGAFGKMMPGIKK"
     gene            5509..5955
                     /gene="rpsP"
                     /locus_tag="PLO70_00910"
     CDS             5509..5955
                     /gene="rpsP"
                     /locus_tag="PLO70_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQZ73040.1"
                     /translation="MSAKIRLQRHGSKKRPFYFIVIADSRAPRDGKFIQKLGTYNPLT
                     VPATIQLDRQKALEWLNKGATPTDTVRRILSFKGVLYLKHLLRGVGLGLFDDATAMEK
                     FTKWSAEHEAQVKKRQEDAYRNKRARRAVPGRRPERRTEGEGGSEG"
     gene            6058..6576
                     /gene="rimM"
                     /locus_tag="PLO70_00915"
     CDS             6058..6576
                     /gene="rimM"
                     /locus_tag="PLO70_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789003.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQZ73041.1"
                     /translation="MTEYFNIGKLVAVHGLKGELLLKHELGKKTSLKGLQAIFIEEKK
                     NSFIPYFIEATKIKSEDEVYIKLEGVNTREVAIKLTQKQAWLPEPDFKKFAAKSAPAS
                     LLGYTIIHKNESLGEILELIEQPHQLLCRLDIKGKEVLIPLHEETLQKVNHKKKEVLV
                     ELPDGLLEIYLT"
     gene            6627..7832
                     /gene="dinB"
                     /locus_tag="PLO70_00920"
     CDS             6627..7832
                     /gene="dinB"
                     /locus_tag="PLO70_00920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQZ73042.1"
                     /translation="MEEAKHHIAHFDLDSFFVSVEIRNNPSLKGKPVLVGGYERGVVA
                     ACSYEARKFGIHSAMPMKKAMQLCPQAIITNASRDQYSKYSRWVTEIIASKVPLFEKA
                     SIDEFYIDLTGMDKFFGVSQYAKDLRSLITNETGLPISCGLASARFIAKMATNEAKPN
                     GFLEIPHGKEKEFLWPLGIEKINGVGKQTEQQLKSYGIYTIEDIAKTPIEHLEKIAGK
                     WGEALWHKAHGIGSTAITTDWEQKSMSHENTFDTDYTDVSFLHKELVRLTEKTAYSLR
                     EDEKMTGCITVKIRYSDFETTSKQETVDYTALDDVLIGKVKDLFNKSYQKDRPVRLLG
                     VRFSHMIPITMQMSLFENNVEKLNLYKAVDEIKDRFGSKLISKAVTSQVKDNSNEFTT
                     RHSKKKKPG"
     gene            complement(7845..10247)
                     /locus_tag="PLO70_00925"
     CDS             complement(7845..10247)
                     /locus_tag="PLO70_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73043.1"
                     /translation="MKQIIQLTGLFFFSTICAIAQYTASNEKSSRTDKIDQLPLIRSE
                     NPVAIRYLLQHEGLKGKVKKITLKDSTGKVISQEGYDIRGRLTKKLVGSERSAVFDNF
                     SYDSTTRSILQKTEYATGEKVFYKYVYNSNGDVTNYYIANQAGTIFYSKRVYHYQDNL
                     FVLDSAYNPSAFDYYIIHQYNNEGQIISSKTYTQKARVLLAEIAYEYKIQDGLPLIKI
                     QTRTDYSGNGRFENSTSIKYFDNKQNIIKEQNIGATNFSVRPRELTYKLDAAGNWISN
                     SKRETREIEYYEPLPVTNKETAKTDNKNNSQLSLILDENFDNNNNKWAVWDNEGSAAQ
                     LHKGNYRVTVKQSNNYASWLSVPGLASDQSKDFAIETKMLLNSTETGNPLDSYWLLWG
                     IGDNGKNFYAFGIYPEGKFQYGKLVNSQWDGKAGTIASTSINAGINKANVLRVEKRKD
                     TISFFVNGSKVHKAKYEIFNSSHTGIGFQWNNKKLVDIDYLKIFQGSVSAITLSPEPQ
                     ESNYQKALAKAGNSKERADAIIDYYLALKALNFSADKLEILLGQKFLQMIDIDFHGYY
                     QVLMSRRINFDDVKLCMKASAVLTAEQRNAVGLLNKYAVDEFQATQNNTAKPAYPTGV
                     PQPGYGWGKTISSNKVNNSNGGNKTPPTIVSPAKPKDELAILRGSAKYLQGQMVYLGS
                     QKTCYFTPGTIVINSVNDEITLKTIFAKFSYSYSSGRNEFGISSSTYETTNIKVKDLV
                     RMIDGVSGAYIVTQIGPCKSCGGNPKSWNNNTRKGSYCTHCGATGCAPIAIWNHGSSR
                     AF"
     gene            complement(10304..11362)
                     /locus_tag="PLO70_00930"
     CDS             complement(10304..11362)
                     /locus_tag="PLO70_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73044.1"
                     /translation="MNLSHNRSLQPVYWGLLHGINDLAAGFLLAGYTLSHGYSDSFLF
                     ISIYAILGFGGQLPVGFWLDKQKDIALFAKISVSLLPLSLLFFFVSTEMAIICSGVAS
                     AFVHVTGGTICLQVHENKSGPLGLFTAPGVLGLTIGGLLGEAGYLLPLVLVLAAMLVT
                     ITIFRQPLPVYQTPEKKESELDSHDLVMLLLLLFMCFRSFLFDVVNYVAENYEDGLLY
                     IGVSAFFGKIIGGFVADRVGIRKFIYITLLAALVLLQFGKTNIYMLCTGIALLQSSVP
                     VTLLMMGRSLPFHPATATAFSLGTSVVLAGLPLFLVSDKRLIYDAFSVDWFTALLFVA
                     FLIVLAITGNYVVRKIFL"
     gene            complement(11359..11673)
                     /locus_tag="PLO70_00935"
     CDS             complement(11359..11673)
                     /locus_tag="PLO70_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73045.1"
                     /translation="MNAWWFFALTLLIEFPIILLFYYKQWKEILVPFVLLNLFTWPLL
                     HFLLLTTDISLSLMELGVALAEMAGYKLLMKSSWGRSFAAAFIANGISYGAGLLINNY
                     SL"
     gene            complement(11673..11825)
                     /locus_tag="PLO70_00940"
     CDS             complement(11673..11825)
                     /locus_tag="PLO70_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HQZ73046.1"
                     /translation="MFRFLLDVPPEPTLAEKAKTNIWLIIIALAAMVGIVAFFIWKRR
                     KNKQEC"
     gene            complement(11850..12155)
                     /locus_tag="PLO70_00945"
     CDS             complement(11850..12155)
                     /locus_tag="PLO70_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73047.1"
                     /translation="MPLIIALIGFFLIKGIFQLPFYLRQWKASLLVALFTSLAVVPLI
                     TILYHETSLDFTFVYAGMILADIVVLNLLVQNNWWKAVPAAFIVNTIAILFFFIGNG"
     gene            complement(12169..12741)
                     /locus_tag="PLO70_00950"
     CDS             complement(12169..12741)
                     /locus_tag="PLO70_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73048.1"
                     /translation="MKRVRAFFTALLVLTSLAAMADAGFSIRRRTAPAQITFEGTGNL
                     AGYKLVLVQYSYHEEDTLLKNPFISRRDTIDDNYKIVIQDGGKRWDESERYLHFALAK
                     SDSAGTVTDTFTVYMKKWNYQMVITGAKDSKLQYKMKKSKAYFDYAVIADDDSGSNGR
                     VNRWIFILCSLAGLMLLILLFLKRKTIKPA"
     gene            12887..14257
                     /locus_tag="PLO70_00955"
     CDS             12887..14257
                     /locus_tag="PLO70_00955"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQZ73049.1"
                     /translation="MSQVWKDYQEKNKDRYLNEMLDLLRIPSVSAKSEHKDDMLKCAE
                     AVKKSLLEAGCDKAEVMATDGHPAVYGEKIIDPSKPTVLVYGHYDVQPPEPLELWTNP
                     PFEPTIIDGKIFARGSADDKGQFYMHVKALETLVKTKTMTTNIKFLIEGEEEVGSPNL
                     GKFVAAHKDMLKADVILISDSSMLSMEHPSLDTGVRGLSYIQVEVTGANRDLHSGTYG
                     GAVANPITILAQMIASCHDENNHITIPGFYDDVVIASPEERALINMAPYDEKEYMDEL
                     GVKELWGEKGYSTYERTGIRPTIEINGIWGGYTGEGAKTVLPSKAYAKISARLVPNQS
                     SDKITEKLLAYFKSIAPKSVEVKAELHHGGEPYMTPIDSKGYKAAAKAVETTFGKEPI
                     PVRGGGSIPICSILEKELGIKIIFMGFGLDNDNLHSPNEKYNIENYYKGIETIPYFHK
                     YFAEMN"
     gene            14316..14699
                     /locus_tag="PLO70_00960"
     CDS             14316..14699
                     /locus_tag="PLO70_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQZ73050.1"
                     /translation="MPVKEKLRLDKYLWAIRLFKTRSMAATACDSGKVKFDGSQAKAS
                     RNVSIGDEYEVKTEAKRWRIKVTGLLEKRVAAAEAIKNYIDITPEEEIERIRFQAASF
                     HTGKRQSKIGRPTKKERRDLDEFLE"
     gene            complement(14714..15157)
                     /locus_tag="PLO70_00965"
     CDS             complement(14714..15157)
                     /locus_tag="PLO70_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQZ73051.1"
                     /translation="MFLSKSFGYALRGVLYIALVDKEGRKVQLDEIAEKLSVPRHFLA
                     KIMKMMVKEGILSSTKGPYGGFYLNEVTLKTKLIQLVKITDGVEQFNICVLSLRKCNS
                     KNPCPLHFQMLKLRDGMLAEFSAKKLGDLLDEKNPDFIKTISTIA"
     gene            15357..15827
                     /locus_tag="PLO70_00970"
     CDS             15357..15827
                     /locus_tag="PLO70_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ73052.1"
                     /translation="MKRNILGTVLLSASIFFFSCNEGGVKDEPKPLDVQELTKDKPET
                     HGSEVKEGDITLSTPLDQAMVAAGKGTYELKCQSCHRLTEEKLVGPGWKGVTQKRQPV
                     WIMNMITNVDMMLEKDPEAQKLLELCLVRMPNQNITTDEARKVVEFMRSNDGEK"
     gene            15893..>17790
                     /gene="nosZ"
                     /locus_tag="PLO70_00975"
     CDS             15893..>17790
                     /gene="nosZ"
                     /locus_tag="PLO70_00975"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HQZ73053.1"
                     /translation="MKHSFLKTFFAFSLLLIGLVWLQGCKPKGTQSAAGGDAAKAYVA
                     PGKYDEFYNFVSGGFSGQMSVYGLPSGRLFRVIPVFSVDPEKGWGYSEETKPMLNTSH
                     GFVPWDDLHHIALSVTGSEHDGRWVFGNANNTPRIARIDLKTFRTAEIIEIPNSAGNH
                     SSPFITENSEYVVAGTRFSVPLGDKLDVPINTYKENFKGTISFIGVDKTSGEMNVAFQ
                     ILTPGINFDLARAGKGKSAGWFFFSCYNSEKANTLLEVNASQKDKDFIMAVNWKKAEE
                     YAKAGKGVKKAVRYAHNKYDDNTHSATSTIMNEVLVLDPKDCPDMIYFMPCPKSPHGC
                     DTDPTGEYIVGSGKLAAVIPVFSFTKIQEAIAKKNYDGEFDGIPVLKYEAVLHGEVSK
                     PGLGPLHTEFDANGNAYTSFFVSSEIVKWSVKDLKVLDRVPTYYSIGHLSVPGGPTKK
                     PWGKYVIAYNKITKDRYLPTGPELTQSAQLYDISGDKMKLILDFPTIGEPHYAEAIPA
                     DMIIKNSRKIFKLEENKHPYVAMGEGKTKVERKGNEVHVYMTAIRSHITPDNIEGVKL
                     GETVYFHVTNLEQDWDVPHGFAVKGISNAELLIMPGETQTLKWTPEKAGVYPMYCTDF
                     CSALHQEM"
     assembly_gap    17791..17890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17891..18348
                     /locus_tag="PLO70_00980"
     CDS             <17891..18348
                     /locus_tag="PLO70_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ73054.1"
                     /translation="AGGQEAVQDDESQKDVVKVAVGSADHSTLVAALKQAELVTSLSN
                     AGPFTVFAPTNAAFDKLPAGTVDGLMKDDKKADLQNILQYHVAVAVYKTENMKDGQII
                     GQANGDNITLSVKDGKVMINGSANIVASIPASNGIIHVIDAVLLPPAKK"
     gene            18430..19020
                     /locus_tag="PLO70_00985"
     CDS             18430..19020
                     /locus_tag="PLO70_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73055.1"
                     /translation="MNNKLSVTSRILVAFASGALVTVFFLPAWRIDLFAPQYPEGLMM
                     NIWINGLTGDVDIINGLNHYIGMKHISVDMFPEFKFLPYVVGFYMLLGIVIAITGSRK
                     FLLLYLILIVIGGALAMYDFYQWGYDYGHNLDPSAAIQVPGLSYQPPLFGHKRLLNFD
                     AYSFPDVGGWIVIAASALAFGVWLAEWYHQRKLNKK"
     gene            19017..19457
                     /locus_tag="PLO70_00990"
     CDS             19017..19457
                     /locus_tag="PLO70_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HQZ73056.1"
                     /translation="MRSSFFVAVAAFVLILSSCSAQPEPFKYGQDICYTCKMGIVDPK
                     FGCQLITTKGKIFKFDDVVCLSRFIKSGTVTEKDIRQTVVINFEKENDFLDINKTFFV
                     MAPAIKSPMGSHAAAFSGKEAAEKFNAGKTGQILNWKDLSGKLE"
     gene            19461..20693
                     /locus_tag="PLO70_00995"
     CDS             19461..20693
                     /locus_tag="PLO70_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HQZ73057.1"
                     /translation="MQYFFTIVLSFLLTPVFSRTIIVGPTQPIRSLKKAIETADEKDS
                     IILLKGTYKEGSIILTKSLTIIGQNNPVLEGENKYEILLISGKNIIVKGIEFRNSGYS
                     AMDDYAAIKLVDCSNVTIENNSIRFAYFAIHISNTTRAIIRNNRINGIPTSEQLTGNG
                     IHLWKSSHAIIENNYIQGHRDGIYFEFVTESQIKNNLSEKNIRYGLHFMFSNDDNYQN
                     NTFRNNGAGVAVMYSKRVRMEKNHFEENWGASAYGILLKDITDSHIQNNTFFKNTVGI
                     YMEGSSRIEIQKNFFKNNGWAIKVQASCDDNVFHHNNFSGNSFDVGTNGTMVLNKFNN
                     NYWDKYEGYDMNKDGIGDIPYHPVSMYSMIVEQNPTTLMLFRSFMVSLLDKAEKAIPS
                     LTPENLFDDAPMMKMMKI"
     gene            20690..21448
                     /locus_tag="PLO70_01000"
     CDS             20690..21448
                     /locus_tag="PLO70_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ73058.1"
                     /translation="MIRIEQVNKKFRKLQALDNISANFDRGQVISLIGPNGSGKTTLI
                     KSILGMVRPDSGKIFVKDQPVNGDPAYRKDIGYMPQIGRYPDNMKVGQLFEMMKKIRN
                     ADDSKTDNDLFEQFSIPSIFEKPMRTLSGGTRQKVSAALAFLFNPLILILDEPTAGLD
                     PLSSEILKEKIISEKQKNKLILITSHVLSDLEELTTHIMYLQEGKMQFLKDLETLQEE
                     TGEIKLSKAIARVMKGHETDSKWIDQMFSVKKKI"
     gene            21472..22239
                     /locus_tag="PLO70_01005"
     CDS             21472..22239
                     /locus_tag="PLO70_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQZ73059.1"
                     /translation="MLKVSRYVLYDIFRNKIVIAYTLFLFIVSFSMFRMEENSSKAML
                     SLLSIILIVLPLVSLVFSTIHYYNSYEFIELMLSQPLSRTRILLSEFGGVCISLLSAF
                     WIGVGVPVLIFSADETGFALLFTGTALTIVFTSIAFLASVRSKDKARGIGFALLLWFY
                     FALIYDGLVLLILFAFSDYPMEKFTLLLSALNPIDLGRIFIMLKMDISALMGYTGALY
                     KDFFGSGKGVLFTTVIMLLWAIIPLWLAVRSFRKKDM"
     gene            22270..23013
                     /gene="ric"
                     /locus_tag="PLO70_01010"
     CDS             22270..23013
                     /gene="ric"
                     /locus_tag="PLO70_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HQZ73060.1"
                     /translation="MNELMTKSLAQIVTGNHRTAAIFEKYHLDFCCKGKRSLEQACNE
                     QNIATNELLAELESISSAPVTGTAGFPFDQLTLTQLSNYIVSTHHAYVKGELPQLFSY
                     IEKVAAKHGERHPELYKIFELVVAIKEEMELHMRKEEQILFPRIQEIENIATQDKESP
                     QLNINYLQSPISVMEQEHDHAGALTAEIRQLTSNYTAPPDACTTYKLLFASLQAFETD
                     LHQHVHLENNILFPKAIELFQHLNEVRMN"
     gene            23189..23641
                     /locus_tag="PLO70_01015"
     CDS             23189..23641
                     /locus_tag="PLO70_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQZ73061.1"
                     /translation="METKAVDKPRTSWPTLFDTGWMDKVFNAPIDEFFNIGKVINVPA
                     VNVTETDKEFKLNIAAPGLEKKDFKVEAIDDMLTISAEHEREEKEEKNGRFNRREYNY
                     SSWSRSFTLPENCDSGKIDAEYKNGELKIFIPKLEVKEPKKVKSISVN"
     gene            complement(23801..24862)
                     /locus_tag="PLO70_01020"
     CDS             complement(23801..24862)
                     /locus_tag="PLO70_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ73062.1"
                     /translation="MSQKTILIIDDNEDIRENTAEILSLGGYKTVTAENGKRGVEAAL
                     ASKPDLIVCDIMMPELDGYGVLHLLRKNPETENIPLIFLTAKAERSDLRKGMEMGADD
                     YVTKPFEEIELMNAIESRLKKYDVLQKKYDASGKGLSELANDLRENGMLQFNPDNYNS
                     EIYTKKQVIYAEGKRPRFLYYVVKGKVKGFKTHEDGKEYITDLYSDGDFVGYPALIEE
                     KNYDDSAVALEDTEIVQVPREDFQQMIEGNITVASKFIRIITQNVKEKEERLLSLAYS
                     SLRKRVAKALVDIHGKFNTSGENKPIEISREDIAHYVGTATESLIRTLSDFKSEKLIE
                     IKDGKISISNVEKLKHLLY"
     gene            25001..27397
                     /locus_tag="PLO70_01025"
     CDS             25001..27397
                     /locus_tag="PLO70_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQZ73063.1"
                     /translation="MAAPVITDKEIHCYHCGETCLNDKIRSTEKVFCCEGCKMVYQIL
                     SQSDLCDYYNLNENPGLSQRIKVRKDKFSFLDDEKIQLQLISFRDDKQVHITFYLPQM
                     HCSSCLYLLENLHRLDGGVISSKVNFTRKEVDIVLLTDKTSLRKVAETLTSIGYEPYI
                     SLNDLKEKRPVIDKSMVYQIGVAGFAFGNIMLLSFPEYLGIDATETSLRSVFRWISFG
                     LAIPVLLYSALPFYQSSWKSIKHKFLNIDAPIALAIIITFIRSAYEVISGTGGGYFDS
                     MTGIVFFMLAGRILQDKTYRQLSFERDYSSYFPIAISVLKDNKEVPTALPDIKPGDTL
                     LIHHEELIPADGILTRGKAFIDYSFVTGESLPVLKEMGEIVYAGGKQTEGNIEILVVK
                     EVTQSYLTKLWNRDEFKEQEENKKVSFVNILSRYFTYIVLTIAAATAVYWQINDPSRM
                     WPAVTAIFIIACPCALFLSNTFTNGNVLRILGRNHLYLRNAQTIEEMAGATHIVFDKT
                     GTLTSTQQQDIVYEGLALTAYQLHQVAALADSSNHPLSKALAKHIGKTKEIHVEGFKE
                     ITGQGIEGLVNNDLLKLGSYEYITGEVNRQPGTAVYISFEGELFGYFLFNNHYREAIP
                     SLISELKKEYHLSVLSGDNTAEKDTLQKLLGKKATLLFHQKPEDKLQYIQYLQNQGEK
                     VMMIGDGLNDAGALKQSDIGIAVAEQTNNFTPSSDAIIEAKQLSKLTKFISLCRANRN
                     IVIASFVLSIVYNVIGLFFAVQGTLSPLIAAILMPSSSLSILLLTFGSSSLIARQMKL
                     "
     assembly_gap    27596..27695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27696..29522
                     /gene="ccoO"
                     /locus_tag="PLO70_01030"
     CDS             <27696..29522
                     /gene="ccoO"
                     /locus_tag="PLO70_01030"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQZ73064.1"
                     /translation="IAAAAITLLMGYTTSKEYAELEWPIDIAITIVWVIFGINMFGTI
                     LKRREKHLYVAIWFYIATWVTVAMLHIVNSIAIPVSFMKSYSWYAGVQDALVQWWYGH
                     NAVAFFLTTPILGLMYYFLPKAANRPVYSYRLSIIHFWALIFIYIWAGPHHLLYSALP
                     DWAQSLGVVFSIMLIAPSWGGMLNGLFTLRGAWDKVREDPVLKFMVVAITCYGMSTFE
                     GPMLSLKSINAISHFTDLTIAHVHIGGLGWNGFLAFAILYYMVPKMWGTTLYSKKLAT
                     NHFWLGTIGIILYAVPLYWAGFEQASMWKQFTPEGQIKYDFLQTVTNLIPMYISRSIG
                     GTLYLVGALMMVYNLYKTVKKGIFIPNEAAEAAPLVKEVSHKKEYWHRVIERKPVTML
                     ILSLILVSIGGLVQMIPTFLVKSNIPTIASVKPYTPLELQGRDIYVREGCYTCHSQMI
                     RPFRDEVARYGEYSKAGEFVYDHPFQWGSKRTGPDLAREGAPKLKRPDSWHYNHMYDP
                     RSMSPGSLMPQYVWLFDNKLDTGSTPAKIRAMRTLGVPYPKGYDKIANRDLMHQADSI
                     AANLKKEKIESPSDVEIIALIAYLQRLGRDITNEPKPLAENK"
     gene            29533..29694
                     /locus_tag="PLO70_01035"
     CDS             29533..29694
                     /locus_tag="PLO70_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HQZ73065.1"
                     /translation="MKFINYLEKVSGVDIFGLASLIIFFLFFLVMLTWVFKTKKKSYR
                     EISRIPLDN"
     gene            29725..30906
                     /locus_tag="PLO70_01040"
     CDS             29725..30906
                     /locus_tag="PLO70_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQZ73066.1"
                     /translation="MRKKTKYIPGILASLLIMTAQPVWAAGPPEPSILSNPLALTLMI
                     LMALLLIIIGILANILIGAADVKLKKRNKPSSNKPVAAASLILVLLLASSSLFAQDAA
                     GTADAANTAVKTIGGMSASTFYIMVTVLFLELFIIIALLINIKFLLKTEKEKLATAVE
                     TEEVKANKLSWWDRFNKLSPVSEEADLDLGHDYDGIRELDNRLPPWWLYGFYLCIIFA
                     GIYLWRFHVSHTGPSSKQEYERSVARADAKIQEYLKQKGESVDENTVTLLTSADDIQA
                     GKTIFMDPAKCPACHGTDGGGNAVGPNLTDDYWIYGGSIKTIFKTIKYGANNGMKSWK
                     DELSAKQMAQVASYIKSLRGTKPANPKAPQGELYKEEAAPAAPAADSTAADAKKIAMN
                     N"
     gene            30921..32366
                     /gene="ccoG"
                     /locus_tag="PLO70_01045"
     CDS             30921..32366
                     /gene="ccoG"
                     /locus_tag="PLO70_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQZ73067.1"
                     /translation="MNEEITNEIQPEETPNESFRNRIATVDAKGKRKWVFAQKPQGKF
                     YNIRTWVSWGFFILFFALPFIKMNGRPLFLFNVPDARFIIFGKIFWPQDFFIFGLTMV
                     TFIIFIVLFTAAFGRLFCGWVCPQTIFMEMLFRKVEYLIEGDAAKQKLLKNSPWTAEK
                     IRKKATKHIVFYLLAFIIANFFLAYIIGMDELKKIITEPVSEHIIGFMSILLFSGIFY
                     GVYAFFREQVCTVVCPYGRLQSVLLDRNSMLVAYDHKRGEPRGKYKKNQAAEQTAGDC
                     IDCFQCVKVCPTGIDIRNGTQMECVGCTACIDACDKMMDAVGRPRGLVRYASENGIEE
                     GKKLSYTGRMKFYTILLIVLTGILATLLITRKDIDGTIVRTRGQLYQERGTDSLSNLF
                     NIKIINKTIHNIPVTLRLEGEAAKAGKVEMIGSKEIILKQEDQATGSFFIVLPRNFIT
                     KRKMKLEVGLYEGEKKVTAIKTNFTGPFSRQ"
     gene            32391..32828
                     /locus_tag="PLO70_01050"
     CDS             32391..32828
                     /locus_tag="PLO70_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQZ73068.1"
                     /translation="MNWGNKLLVAFIVFGLGMGYLAYRSMNTNFELVENDYYKQELRY
                     QQVIDGKKEANNLALPVNVEQTADGIILQLPEEMKNKTISGNIWFYCAYDAKKDKKFA
                     LKTNNAGMQSFQPESITPGNYTVKINWSDEEKKYYAEKSLTVL"
     gene            32828..33544
                     /locus_tag="PLO70_01055"
     CDS             32828..33544
                     /locus_tag="PLO70_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQZ73069.1"
                     /translation="MLWAAMISGFTLGAAGSLHCVGMCGPLSLALPTHHLTRTQKFFF
                     LLIYQFGRIITYSGIGLVFGLAGRRIYISGYQQWFSIAMGIFVLALAAIYFIQKHSIH
                     FSFLNRFYQSVQQLISNLLKRSGGPFSFLLLGLANGLLPCGLVYVAVAATLSFTEIAE
                     SVSFMAMFGAGTLPAMMLVGYAGQLVNAEARQLMRKAVPVFITLMGIILILRGLNLGI
                     PFISPEMPQSPGEAAVCHPQ"
     gene            complement(33567..33788)
                     /locus_tag="PLO70_01060"
     CDS             complement(33567..33788)
                     /locus_tag="PLO70_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73070.1"
                     /translation="MNKAEYIPVIAVSVLLLLYSILILTGNLFSVVEIIFLISPFAVV
                     WLVYRVIILGRTDYPELKEDQEWGYSDKE"
     gene            complement(33836..35407)
                     /locus_tag="PLO70_01065"
     CDS             complement(33836..35407)
                     /locus_tag="PLO70_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQZ73071.1"
                     /translation="MYNLNSRIITAWAIFVFAVAIALSFLFKDYKLAVSGMLIIIVLT
                     AFIPGWRPTLVAGVMSMIVMTALVIYFKNDQNEIIKALLSQVYSLFVVVFAVIVVFYL
                     KRMQQNFAYEKTHMASLFENATEGILLTDRTGKITLVNPAAERMFGYTANEMMGEQIE
                     KLIPQRYHGKHHGLREGFYKHPSNRSMGTGRDLYARRKDDSEFPVEVSLSHYMQKNES
                     YVIGFIVDITARKEIEKNLLRQKMELEKVSNDIRKLNADLEGKVEERTIILKEALQKL
                     EESQQELSESLDKERQLNEIKSRFVSMASHEFRTPLSTILSSATLVSKYPSTEEFEKR
                     EKHIRRIKESVNHMNELLEDFLSLGKLEEGKVGITVTTFCVRDFIEDVVDEMKAHLKN
                     GQEILSECSGDPNFTTDKRMMKNILLNLLSNAIKFSQENKTVTIQSVVSNRELIITVK
                     DRGLGIPAEDQPHLFSTFFRAKNVSNIQGTGLGLPIVKRYVNLLGGDINLKSELEKGT
                     EVTVSLPEYKEPPIL"
     gene            complement(35468..36223)
                     /locus_tag="PLO70_01070"
     CDS             complement(35468..36223)
                     /locus_tag="PLO70_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HQZ73072.1"
                     /translation="MKVNHSIVIDENACAADIVKQHYRTATVFRKYGVEFCCGGKWPL
                     KMVCETKGLDVEEVLHDLKRASRVLNIPGTLPFDTWKVDFLTDYIVNIHHEYLRQSLP
                     SVKEQLGKFVAEHLKKYPHLPEVESQIEFLDRTMIPHLLQEEEIIFPYIRQIAHAYDS
                     KESYASLLVRTLRKPVEDIMHHEHETLEKTLHRLRFLTNDYTAPDSACTSHRLAFSLL
                     KELDDDLVQHLYLENDILFPKAIAMEKELLERH"
     gene            36529..36909
                     /locus_tag="PLO70_01075"
     CDS             36529..36909
                     /locus_tag="PLO70_01075"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQZ73073.1"
                     /translation="MREVAFIIGGIILLGACSKNTSADKDTELPVITISTPSNNQLFN
                     NGQSINITGSITDNTYIAEVHIHVSNTNTGALLMDVHLYPGSNSTTFNQSLTAISGIN
                     YKIQIIAKDRAVNEARSSVEASCN"
     gene            36930..37610
                     /gene="trmD"
                     /locus_tag="PLO70_01080"
     CDS             36930..37610
                     /gene="trmD"
                     /locus_tag="PLO70_01080"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQZ73074.1"
                     /translation="MHIDILTVLPELLESPFQHSMMKRAQDKGLLTIQVHQLRKWAVN
                     EYGQIDDYQYGGGAGMVMMCEPLAKAIEELSLQKKYDEVIYLTPDGNTFNQKTANALS
                     LKKNLLLICGHYKGIDERIREHYVTKEISIGDYVLSGGELPAAILVDAIGRLIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPVDFRGWKVPDALMSGNHKLIDEWRHEQSIKRTQDR
                     RPDLLKDQ"
     gene            37665..39185
                     /locus_tag="PLO70_01085"
     CDS             37665..39185
                     /locus_tag="PLO70_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HQZ73075.1"
                     /translation="MKKKYFYPVVAITIFGLLFSCTSPKKTVSDQTSSQQFPKALAEF
                     RAAWVATVANINWPSKPGLSTDEQKKEAIELLDFLQQHHFNAVILQVRPQCDALYKSD
                     LEPWSYYLTGKQGQAPFPYYDPLEFWTEAAHDRGIEMHVWLNPYRAHHKDGKEITEHS
                     IIKKRPELALYLKEGYWWLDPAQKGTQDHSTAVVMDLVKRYDIDGVHFDDYFYPYPSY
                     NLGADFPDSVSWNAYQQSGGKLSRGDWRREAVNVFIENLYKKIKAVKPFVKFGLSPFG
                     IWRPGYPESVAGFDQYEQLYADAKLWLNKGWIDYFTPQLYWPINRQNASFPVLLGWWA
                     GENTMKRHLWPGMSVGRDTSIRSVNETLNQIMITRGMVPESKGAVHWSISSVTKNPNL
                     AKALLESTYKNQAIVPASPWLDNKAPDAPKVSTSKTDKITISWSHPDERDVFRWVVYF
                     KYGNNWNYTILNRNERSFTLNPGTEVNKKIVDLKEVAITAVDRTGNESARNQTTIN"
     gene            39247..40695
                     /locus_tag="PLO70_01090"
     CDS             39247..40695
                     /locus_tag="PLO70_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73076.1"
                     /translation="MSTNRRNFIRRIAIGSGVLATGLPSFAKVIGASDEHMERSVDFE
                     KSGQRFNMCGYAAPKIETVRIGIIGLGMRGSDAVERLSYIDGVEIVALCDKYPDRVAA
                     SQKTLEKMGRPKAIAYSGEEGWKALCESSDIDLVYTPTPWHLHTPVAVYAMMNGKHAA
                     VEVPAGKTIYELWELVETSEKTKKHCMMLENCCYDFFELLTLNMARQGMFGELVHAEG
                     AYIHDLSKDWNFNKKAYADMWRLKENIGHNGNLYPTHGLGPIAQCLNINRGDKFDHLV
                     SMSTNDFSMNNIAKELAAKDDFFKEFVDRPYRGNMNTTLIRTNRGKTVMVQHDVSTLR
                     PYSRIHLVSGTKGAAQKWPGPQRIAFGHSWLKPEELKQLEEKYALPIVKHIGAIAKEV
                     GGHGGMDFIMDWRLIDCLRNGLPLDQDVYDAASWSCLMPLSERSVAKKSRTVDIPDFT
                     RGAWQTNKPVDLTLNGGATTGVRSIKPDLKMQ"
     gene            40872..41912
                     /locus_tag="PLO70_01095"
     CDS             40872..41912
                     /locus_tag="PLO70_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQZ73077.1"
                     /translation="MKALTVITDPVYVEPKEFTGYEKFWLKYINDKRDLPFIHLLTAI
                     HILVIPVAILLYTPVLQSWYWWLVYIPYFYVSQMYFKGRFGLMLHCISHRKLFKKGYT
                     WLYNYVIWFVCPFFGHTPETYFAHHMGMHHVENNMLDDASSTLPYQRDSIRGFLSYVG
                     RFLILGFEDTFMYFFKRKRKKFYMRLTAGEISFYLFCVGMSFVNFKATLFIFIIPFVF
                     ARIVMMLGNWAQHAFVNLDDLEDNTINCINTKYNQMCWNDGYHAIHHVRPAMHYTDIP
                     GEFLKTKDQLAEKKVLVFDGIHYLHIFLWLMTKRYDKLADNLVNINNMFNSKEEAIDL
                     MKQRTRKYRMTA"
     gene            complement(41948..45085)
                     /locus_tag="PLO70_01100"
     CDS             complement(41948..45085)
                     /locus_tag="PLO70_01100"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73078.1"
                     /translation="MRKAILLPITIGLAFILAAQKTKPIPPVVIENKEEILFKNLKYR
                     LIGPFRGGRSAAVAGSYKNKTTFYFGGTGGGVWKTADGGSNWTNISDKYFGGSIGAVA
                     VAPSDETILYVGEGENTMRGNVSEGLGGMWRSEDGGRTWKNIGLNDGRHIIRIIVHPK
                     NPDVVWVAVMGHLFGPNQERGVFKTTDGGKTWKKVLYINNQTGCSDLVMEPGNPSVFY
                     AGTWRLIRTPHSLESGGEGSGLWKSIDGGETWTNISTMKGLPKNTWGIVGVAVAPSNT
                     DKIYALIENEKGGLYVSTDAGETWTLASSDNNIRQRAWYYTKVYVDPKNENKVYCPNV
                     NFMISTDGGKTFRSIPTPHGDHHDLWIDPEDGNRMIVADDGGGQVTFDAGNNWSTYLN
                     QPTAQVYRLSTDNAFPYRIMGGQQDNSAFRIRSRTYGAAITAADMETAAGSESGYVVA
                     DPENPDITYGGNYMGMLQRLDHRTGESRLINVWPIDNMGAGAEAAKYRFQWNYPIFFS
                     PHNPKRLYAAGNHLFVTENEGKSWEQISSDLTTNDKTKQASSGGPVTKDNTSVEYYCT
                     VFTAAESPYEKDLLWSGSDDGLIHVSKNGGKNWENVTPKDCPPWMMWNCVEADPFKKG
                     AAYFVGTRYKLDDFKPYIFKTEDYGKTWKQITNSIPPLHFTRALRADRKRQGLLYAGT
                     EYGIYISYDDGANWKSFQLNLPVTPVTDLAIKNNDLIIATQGRSLYILDDLSVVQQRN
                     ENITTKNLHVFTVNPAYRMPASGRFGGFGGTMRNVGINPPNGVVMNYFLKNITDSTRL
                     TVEISDKNKKLIKTFATNSKENKIEINKGMNQFTWDMNYPPADRVEGLILWNGFVRGP
                     KAAPGEYFAKFKSGSDSVEVGFAILGDPNYKTTNADYEEQVNFLLTVRDKFSEVMKAM
                     KNIKEVRQQLTDFNARIGKDIPSEIKQKIDTINKQMTAVEEALHQTKAKSGQDVLNYP
                     IRLDDKLASVYNAASAGNTAPSRQAKEAYAELAVQTDAELTKLKKIVEEDLLKLNQLI
                     HEKTLPVIGLSKEME"
     gene            45145..46776
                     /gene="ade"
                     /locus_tag="PLO70_01105"
     CDS             45145..46776
                     /gene="ade"
                     /locus_tag="PLO70_01105"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HQZ73079.1"
                     /translation="MSSFSISGNLVDVHQQRIYPAELTVDKGKIISIKEISHSSLNTP
                     HFLLPGFIDSHVHIESSMLVPSEFARLAVVHGTVATVSDPHEIANVCGLEGVEFMIEN
                     GKTVPFKFNFGAPSCVPATTFETAGAVLDSTAVETLLRRKEIKYLSEMMNFPGVLSRD
                     EEVMKKIAAAHRLGKPVDGHAPGLRGEKAKQYIDAGIYTDHECFTAEEALEKLQYGMK
                     IIIREGSAAKNFEALIDLLQDYPDMMMFCSDDKHPDSLESGHINQLCARAAAKSIDIF
                     KILKAACVNPVHHYKLDVGLLREGDAADFIAVKDLKKFEVVKTIINGELVAENGISNI
                     KASKSGIVNNFSCSKKETAHFEIPLTTYNYQLPVIEALDGQLITNRLSFEPTVKDDKL
                     VSDTDRDILKIVVVNRYNDAPVAKAFIKNFGLKHGALASSVAHDSHNIVAVGADDESI
                     CNAVNLVIEHRGGVSFSNDDLEMIVPLPVAGIMSNEDGYKVAEQYSLIDKAVKEAGST
                     LGSPFMTLSFMALLVIPHLKLSDKGLFDGDAFCFV"
     gene            46918..>49181
                     /locus_tag="PLO70_01110"
     CDS             46918..>49181
                     /locus_tag="PLO70_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQZ73080.1"
                     /translation="MVKDALRIAELEKLLAERTAELIQQGEELAVINSVQEALVKEMD
                     MQAIYDLVGNKIQELFDAQAVIISTFDHENGLEQFQYTIEDGERFYPESRPINKLRKH
                     LIEYKQKILINTSAEAQEWFGKEVVPGTKLMQSGVFVPLVTGDIVTSYVSLQNVEKEY
                     AFSDSEVRLLETLANSMSTALQNARLLKETEQRTAELAVINSVQEGLAKELDIQAIYD
                     LVGNRIRELFDAQVVGIATFDHEKATEHIHYLIEKGERYYPTPKPINSLRRHIITNRE
                     KLVINKNFATVADTFGIKPVPGTQQPKSAVYVPMLIGDKITGYVSLQNIDKENAFSES
                     DLRLLETLANTMGVALENARLFDETTRLLKETEQQKAELGVINSVQEGLAKELDMQAI
                     YDLVGDRIRALFNAQAVIISTFDHDTATEHFKYLIEDGQRFYLASRPYDKLRQHLIVN
                     RQKIVINSNYAEAYAKFGLKTLPGTNMTKSGVFVPLIIGDKVNSYISLQNNETENAFS
                     ESDIRLLETLANSMSVALENARLFDETTRLLKETEQRTAELAVINSVQEGLARELDMN
                     AIYDLVGNKIRDVFDAQAVVIATFDHENGTEKFNYLLEKGERYYAEPRRYDKLRYQLI
                     MTKHRILINDDVEKAFKEFGLKVVEGTEMPKSLLYVPLVVGNKVTSYVSLQNVDKNNA
                     FSDADVRLLETLANSMSVALENARLFDETTRLLKETEQRTAELAVINSVQEGLAKELD
                     MQGIYNLVGDRLCE"
     assembly_gap    49182..49281
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49282..51356
                     /locus_tag="PLO70_01115"
     CDS             <49282..51356
                     /locus_tag="PLO70_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ73081.1"
                     /translation="TESDMRLLTTLTNSMSVALENARLFDETTLLLAESKQRAAELST
                     VNSISKALASQLNADDLIKMVGDQLKNLFKANIVYLALLNSKTNIVSFPYQYGDNMAP
                     LKLGEGLTSRIIMTGEPILINKDLQELREQMGIQSIGVPAASYLGVPIPVGDENIGVL
                     SVQSTEHENRFNENDLRLLTTIASSVGVALNNATLFEDVKLAKMEAEAASNAAEKANE
                     AKSAFLSTVSHELRTPLTSVLGFAKIIRKRLDEKIFPIVDRSDKKTDKTISQISENLQ
                     VVISEGERLTHLINDVLDLAKIEAGKMEWSQENVSMPEVAERAIAATTSLFDQKALKL
                     VKEIDTNLPEITGDKDKLIQVIINLISNSVKFTDKGTVTCRAYQEKDELIVSISDTGI
                     GIAPEDYGAVFEQFKQVGGDTLTDKPKGTGLGLPICKEIVEHHGGRIWVESEVGKGST
                     FSFALPVNKTTAHARPLHLDDLVKQLKEQVAHSQLNIIGQASNILIVDDDDSIRSLLQ
                     QELGDAGYSIEEASNGKEALEKVRINRPDLIILDIMMPEMNGFDVAAILKNDPNTMDI
                     PIIVLSVVQDKARGFRIGVDRYLTKPIDTGLLFNEIGSLLEQGKSKKKVMIVDEDSGA
                     VKTLTDVLTTKGYEVTESIEKELVEKAIANQPDIIIINSALSGKHDIVQTLRFEKGLE
                     NVLFVVYQ"
     gene            51551..51934
                     /locus_tag="PLO70_01120"
     CDS             51551..51934
                     /locus_tag="PLO70_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ73082.1"
                     /translation="MEQKILIVDDEAHIRMLIGQTLEELEDEGVEFLTAENGEIALDI
                     IQKEKPQLVFLDVMMPKMNGMEVCRRVKQELKMENVFIILLTAKGQELDRQKGQEVGA
                     DVYMTKPFDPEVLLIKAREVMGLAN"
     gene            52002..53681
                     /locus_tag="PLO70_01125"
     CDS             52002..53681
                     /locus_tag="PLO70_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ73083.1"
                     /translation="MTRISLKNIIGKKNEAVAMILALIEQLNIIAWIEDDAGKILLGT
                     ARETADSIFPVIAEEEILGFVKGEQKAAIIARLLTHLITKESEKKKLGSEVLHLYQEV
                     NLMFDFSDKLAKTIDAADIAKTTLEEASRVIRSDNGVIILWDEKNRQLQVLASHGNLF
                     FNQEKINTELKILLDLILNGQSEIISDTSALRATGIVRPHISSVLYSALKVNHRVMGA
                     VILASNEEIQYTAADLKLLTTLALQSSAAIESALLYERNIKDALEREEAMRKLYDATG
                     KFVPYEFIRSLGHQLITDVKLGDQVEKIVTVLFSDIREYTTIAEQMTPEENFRFVCSF
                     NERMGPFIRQYNGFINQYLGDAIMAIFPGNATDALAAAVAMQKEVEQFNIARLLNNQT
                     PIQIGVGMHTGPLIMGITGDKDRMDACTISDTVNTASRLEGLTKHYKTSIILSDASLE
                     QINNKENFHLRNLGMVQLKGKQESIKIHECFSGNTPKDIQKKAATLSLFNEGISHYLD
                     KSFNKAHEVFTQVIKENPEDRTAAFFFTNTKQVMEEGFSKNREGIVEMQEK"
     gene            53837..54421
                     /locus_tag="PLO70_01130"
     CDS             53837..54421
                     /locus_tag="PLO70_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQZ73084.1"
                     /translation="MLFESFHFIHDHVVHNLLTGLSEKLTYHNLPHTLDVLKQVQRIG
                     KEEGIMEEEDMFLLKTAALYHDTGFLSVYAGHEAAGCELARRDLPGFGFSKNQTEQVC
                     SLIMATKIPQSPKNILEEIICDADLDYLGRDDFEPISDSLRREFLELGIVSSDEHWYS
                     LQIKFIENHNYFTNSSRQLRNPEKLKRLEKLKSV"
     gene            54717..55013
                     /locus_tag="PLO70_01135"
     CDS             54717..55013
                     /locus_tag="PLO70_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73085.1"
                     /translation="MKQYLHISATFDQPELVQNLVEQLAYRRYELGFSNEKMPRGFKA
                     LETSSTETYVSGTMAIDFGTNEAPVNMPFITCFLFTCIKGKTDPYQLIWSSSLS"
     gene            55146..55955
                     /locus_tag="PLO70_01140"
     CDS             55146..55955
                     /locus_tag="PLO70_01140"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HQZ73086.1"
                     /translation="MDSYNDNREQRREERKERRQFYMTNSSRHSHIWTGLFILLVGVA
                     ALIKASVTDLPSWLFGWQTFLIALGIFIGFKHGFRGGAWFILILVGSAFLLRDIYPDL
                     AIRRYIWPMILIVIGAFLIIRPRRKSWEHCAWNEKKNTGSLNSTSILGEEETWSKDDF
                     VDSTSIFSGDKKNILSKDFKGGDIVNIFGGTELNLSQADIQGKVAIEITTIFGGTKLI
                     VPSHWEVKSEAVTIFGGLEDKRSITAIPESGGKVLLLKGTVIFGGIEIKSF"
     gene            56018..56377
                     /locus_tag="PLO70_01145"
     CDS             56018..56377
                     /locus_tag="PLO70_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HQZ73087.1"
                     /translation="MNWYLAKMVFRIICGEGNHTAQFDEQLRLISGESKEEAFHKAHM
                     MGMKEEEIFFNKNMQLVQWQFISVSELYQLNELIDGAELYSKIEERENADSYLHIVRK
                     KAEQIRFGNSLEMLNPA"
     gene            56379..57419
                     /locus_tag="PLO70_01150"
     CDS             56379..57419
                     /locus_tag="PLO70_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ73088.1"
                     /translation="MSNSPLSILRFKVIFIICWLVIITDQAIALNFFGLPWQAAIIDS
                     LICNALLLLACLLIMHTLRYYLPRGQQYINIFSICIFLSILWLILSKWLLKLSLGHYP
                     GYTDMLHQSLSIRFSIGFLLLGCITMISILWYNQQEQKEKDTRKTDAEKLAKEAELFK
                     LRQQLQPHFLFNSLNSINALIGSRPEEARKMVQQLSDFLRGTIKKEETQWVTLQEELQ
                     YLQLYLDIEKVRFGNRLATDINIDENTQSMKLPALLLQPIVENAIKFGLYDTTGETVI
                     RLYASREENNLVIRVMNPFDPETSSPKQGIGFGLKSVQRRLYLLFARTDLLSTEAKEN
                     IFITVVKIPETT"
     gene            57491..58231
                     /locus_tag="PLO70_01155"
     CDS             57491..58231
                     /locus_tag="PLO70_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HQZ73089.1"
                     /translation="MGKIIIIDDEPLARSIVREFLQKHPQLEVIQECGDGFEGVKAIQ
                     QHQPDLIFLDIQMPKITGFEMLELIEQPPGVIFTTAFDEFAIKAFEAHAIDYLLKPFN
                     QDRFDKAIGKWTEQRSGTTEKATQDLLESASLSPSQRQRIVVRNGSKIKIISVHDVFY
                     LEAADDYVKIHTQEGYFLKNKTMNHFEQSLDIHQFVRSHRSYIVNVQQITRIDPYEKD
                     NHVAILRSGAKVPVSRSGYSKLKEVLGL"
     gene            complement(58235..59686)
                     /locus_tag="PLO70_01160"
     CDS             complement(58235..59686)
                     /locus_tag="PLO70_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HQZ73090.1"
                     /translation="MPQYKAVFHDRMAAKTVVVIPSLTLDPFILAKIDGIVHYEERLL
                     CLLLLLRMPRTHIVYVTSTPVDPLILDYYLHLLPGITGEHARQRMHFFSCYDSSPVSL
                     TEKILARPRLIKRIMNAIPDGHLAHLACFNVTEHERTLSATLGLPVYGCDPDLLFWGT
                     KSGSREIFRECGMEMPPGYENLQNEEQIIKALADLYIADPLLKKAVVKMNDGFSGEGN
                     AVFPFDTITSNENVEEEIRQLLPRVLEIVATDLSYELFIDKFTSMGGIVEAFIDAPHK
                     TSPSVQCRINPLGKIDIISTHDQLMGGPDGQVFLGGTFPADEEYSVEVAALGRKVSEV
                     MKRKGVLGRFGVDFLSVKENDQWKHYAIEINLRKGGTTHPYIMLQFLTNGDYNPDTGK
                     YVLVNGDEKCYLFTDNLHEDRFKGLTPCDLMEIAMLNNLHYDNTKEEGVMFHLIGALS
                     QYGKIGLVCIASTFERAKYFYDQTLKVLNSECY"
     gene            complement(59864..60967)
                     /locus_tag="PLO70_01165"
     CDS             complement(59864..60967)
                     /locus_tag="PLO70_01165"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQZ73091.1"
                     /translation="MVYRAIGLMSGSSLDGLDIAFVEFQEQGGKWTYEIIQADCYSYS
                     AKWVERLRNATNLSAREYQILHTDYGHYTGQQVNRFIDVYQLHYKVAVIASHGHTTFH
                     LPAQKMTAQLGDGAAIAAETQLPVVSDLRALDVAFGGQGAPIVPIGEKLLWTEYAYFL
                     NIGGIANISVNANPYVAFDVCPANRVLNMLANNAGKVYDAGGEMAASGSVNVVLLEKL
                     NDLAYYNQSYPKSLANDFGTDVIFPMIRDSGCSIVDGLRTFVEHIAIQIKRAITSVQL
                     FTPDLVPTGSLLLATGGGALNTFLIERLNSHLKDLKIEVAVPDERLINYKEALIMAFI
                     GVLRWRQEYNVLSSVTGAARDSIGGALWTGQEA"
     gene            61011..61661
                     /gene="upp"
                     /locus_tag="PLO70_01170"
     CDS             61011..61661
                     /gene="upp"
                     /locus_tag="PLO70_01170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQZ73092.1"
                     /translation="MIVNLSQQHSLISNWVGELRDVTIQSDRMRFRRNLERIGEVAAY
                     EISKVLPFEEKEIQTPLGTAVHKVLKEYPVLATILRAGLPLHQGLLNYFDQSDNAFIS
                     AYRKHHKDGSFDISLDYVSCPELENRIVIISDPMLATGSSLVKTIHFLKDEGQPREIH
                     IVAAIACTVGIEYVKREEPSVTIWCGDIDDELTAKGYIVPGLGDAGDLAYGPKMQM"
     gene            61939..62952
                     /locus_tag="PLO70_01175"
     CDS             61939..62952
                     /locus_tag="PLO70_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQZ73093.1"
                     /translation="MKKNLLTLLVCVAMASASRAQDPNFSQFFASPLTLNPALTGKFD
                     GSFRVAGNYRNQWPTINNAFVTKTVSVDFGIMKNRLADIDQLGVGILGVTDRAGDGVL
                     VTNYAGGSVAFHKGLDEDGFHQIGAGFQGTYVNKRLDVTKIDFQDELTPLGFTGVTSE
                     IFLNNQLNISYFDLNAGVLYNGSTNGYNNFYIGASMYHINRPKESFQGGEYLLNTRTT
                     IQAGGKIPVGSYNYLHIAANHSMQAKANNTVVGGAFSYNVNNNEDDPVNVYLGAWYRF
                     NDAAIPYIGLEFKGIQIGASYDANTSSLKPASNTRGGMELSLIYIKKPTDPNAKKLNC
                     PRF"
     gene            complement(63016..64485)
                     /locus_tag="PLO70_01180"
     CDS             complement(63016..64485)
                     /locus_tag="PLO70_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HQZ73094.1"
                     /translation="MIKVKKYQVQTISLLGGIVIALFLYFSNPFGVEHTASKVLAVAA
                     LMITWWVTEALPMPVVALLPLVLFPLLKISKLEAAASGYANPVIFLFMGGFMLGLAIE
                     KWNLHRRIALNIVRLTGTSGDRIILGFILATGLLSMWLSNTATTMMMFPIALSVIHVM
                     KENNTGNGNIANFSLTIMLAIAYASNIGGIATIIGTPPNVAYIGYIEKKFPDSSVDFV
                     NWMLLCMPMAIILLICLYWVMVKWLFPSRIKSDTATKGLIRNEILLLGPLSVAEKRVL
                     IIFISTALLWITRSIINNAQSFIKLDDTIIAMLCALALFICPSGKRESKDEEGTGSLL
                     EWNDTAKMAWGILLLFGGGISLATALENAGLIEQLGGWLAQFSGNGFILVFTITVITI
                     FLSEVMSNVAQVIVFAPVVTSLAIALKLDPLLLGIPMTLAASCASMLPMGTPPNAIVF
                     ASGYIKLRQMTKAGFIMNIVSIILITLFSWFLLPLLMKI"
     gene            complement(64566..65171)
                     /locus_tag="PLO70_01185"
     CDS             complement(64566..65171)
                     /locus_tag="PLO70_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HQZ73095.1"
                     /translation="MKKLPASFYQRENILQIAKELLGKVLVTNWNGKVTSGRIVELEA
                     YNGIIDRASHAFGGRRTNRTEIMYAHGGVAYVYLCYGIHHLFNVVTHSKDVPHAILIR
                     AIEPIEGIAEMLVRTRKKKADYTLTKGPGNVAKALGIVTAHSGYSLQSKELFIADDGF
                     VVSAKQLMSTARIGVDYAGDDALLPYRFYLKDNLYVSGKLK"
     gene            65278..66024
                     /locus_tag="PLO70_01190"
     CDS             65278..66024
                     /locus_tag="PLO70_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HQZ73096.1"
                     /translation="MLKEIVIAFQSWEEARRFIQKNRLFKWIIIPGIIYTILFIAGMF
                     FFWQSSDNVVSWISNQLGIENWLQKERSEWLSFLFVMTGMMLRLVLVLFYFSLFKYLI
                     LIIGSPVFTYLSEKTEAIIDEKEHSFNWPEVKKDAIRGVKLALRNAGWQSVYLIALIF
                     LSLVPVIGWITPVIALLIECYYFGISMLDYSFARNNFSLSQSTAFTGRHKGLAIGNGL
                     LFYAMHILILLAPAYAIIAATLSVHKVKNS"
     gene            66035..66730
                     /locus_tag="PLO70_01195"
     CDS             66035..66730
                     /locus_tag="PLO70_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQZ73097.1"
                     /translation="MTTVYLIPAILQDEGFDAIPPYIRDAIKICQVFFVENERTTRRY
                     FKQLWKEMIIDDYEWVNMTESVIAADTFRKKIKEGKNIGIVSEAGCPGVADPGQALVA
                     IAQEMNVTVKPLVGPSSILLALMASGMNGQQFKFCGYLPIDNLLRIKAIKELEAESAR
                     KNCTQIFIETPYRNNQLADAIIKNCNPSTKLCIAVDLTGKNEWIKTQLISDWEKKKPD
                     IHKRPAIFLLHAK"
     gene            complement(66733..67197)
                     /locus_tag="PLO70_01200"
     CDS             complement(66733..67197)
                     /locus_tag="PLO70_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73098.1"
                     /translation="MDEQQLTQQEQLLAREPGKKKSWFRKFARTILILLFIAVAVLTY
                     FKFFFVFGVGSKAGELNFFVKKGYIFKTYEGRVIQTGYKSKVPGSIQSNEFEFSVVDE
                     KVALQLMSNSGAFMELHYKEYLGALPWRGMSKYVVDSIISIQRPSNTPTPFE"
     gene            67283..67405
                     /locus_tag="PLO70_01205"
     CDS             67283..67405
                     /locus_tag="PLO70_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73099.1"
                     /translation="MANILTHTKQFAFHAPITSGVYSVGFDVFTFSRPRRRPVF"
     gene            67502..68215
                     /locus_tag="PLO70_01210"
     CDS             67502..68215
                     /locus_tag="PLO70_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ73100.1"
                     /translation="MDNQNIIIRVATVSDKVYSKTITDEMAASAAARGTGIAKRSPEY
                     IEQKIDEGKAVIAITGQNEWVGFCYIETWSHGEYVANSGLIVAPAFRKSGVAKSIKKR
                     IFDLSREKYPEAKLFGLTTGLAVMKINSDLGYEPVTYSELTQDEAFWAGCKSCVNYEI
                     LMSKDRKNCMCTAMLYDPKDHYEPEETKQFFEENKKGFERLLKFKQWKLLKPFMKKDK
                     DGNGGASRPKSLLHHLFHL"
     gene            68235..69440
                     /gene="argG"
                     /locus_tag="PLO70_01215"
     CDS             68235..69440
                     /gene="argG"
                     /locus_tag="PLO70_01215"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQZ73101.1"
                     /translation="MAKKVVLGFSGGLDTSYCVKYLSEDKGYEVHSIIVNTGGFSDKE
                     LKQIEAHAFKLGVKTHTTIDAVKGYYDRIIKYLIYGNVLKNNTYPLSVSAERLSQALH
                     IAEHAKKLNADSVAHGSTGAGNDQVRFDMIFHIMIPGVEIITPIRDLKLSREEEIAYL
                     KSKAVDMNFEKAMYSINKGLWGTSVGGKETLSSKGMLPEEAWPTQVTKSGVEEVRLGF
                     ENGELKSVNGNSFTHPVEAIQYLQTIAGPYGIGRDIHVGDTIIGIKGRVGFEAAAPMV
                     ILKAHHALEKHVLTKWQLQWKDQLAQFYGNWLHEGQILDPVMRDIEAFLENSQQNVTG
                     EVFVQLMPYRFQLIGIESKYDLMSSKFGKYGEMNSGFTGDDVRGFSKIFGNQTSIYYQ
                     VKEDADGKN"
     gene            69427..>70231
                     /locus_tag="PLO70_01220"
     CDS             69427..>70231
                     /locus_tag="PLO70_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQZ73102.1"
                     /translation="MEKIKAGIIGGAGYTGGELIRLLLNHPGVETSFIHSRSNAGKSV
                     CSVHQDLAGETDLQFTAELNDDIDVLFLCLGHGESRKFLTEKKIADKIKVIDLANDFR
                     LASSSLIDNKQFVYGLPELNREKIKSANHIANPGCFATAIQLGLLPLAKAGLLNDIYT
                     TGITGSTGAGQTLSTTSHFSWRANNIQAYKTLSHQHLGEIGESLLHLQPANDIDLSFV
                     PWRGDFTRGIFISSTTNCDLSIEELHVLYSTFYAAHPFIYVSKEPIFLKQ"
BASE COUNT        21746 a        14108 c        14142 g        19935 t
ORIGIN      
        1 aaagaactac taaaactatt gaaatacccg ggcaatttaa tacagctttc ctaagccagt
       61 tccaatcact tagtaaacgg gattaaacaa tgagtaaacg ggtgatcaat ttcaaaattg
      121 aggaattaca agggtttcac ctataccaca aaaaggtgat ttttatctat tccgggtttg
      181 ggctgtacga taaatttatt ttcctgccct tctttgaaag aattgcccga taaccctggc
      241 aatccgttta taggcatcct tgttaaaaag ctgggagtct ctcatggctt tgtaattaag
      301 aaaaagcccg actggcacca ggacaaaagt ggccatccac ataccagcaa aaggagttaa
      361 tgtgttctcc ttggcgaatt tttcacctgt tgtactggta aaataaaaca acataaaaaa
      421 gatgatggcg aagatcaatg gtgttcccaa tcctcctttc cggataatgg agcccagcgg
      481 ggcgccgatc aggaatagca ctaaacaagc cattgacagg gcaattttcc gatgccattc
      541 gatcttgtgc ttacgaagat tcttttcctt atccttcatg gtttgcgtca gcgattctgc
      601 gctgatacga acagagcctg ctgtgctttt tgccatctgg ttaatattga tccttgcaga
      661 atcgggtaag atatcgtcaa aactctttac ttcttttaca atgctgctat ctgcagtttc
      721 cggaagtttc caggagctgt ctgtcaatgc agaaaactgt aagagggcaa acatatcttt
      781 ggttgtttgc tccctgatcc ggccgttttc ctttttcagg gagtcgatag ctttatccag
      841 ttgccgcata ctgaacattc tttcattatt cttatttaca ctgtctgccg tacggttagt
      901 aaaaccaaga ttactaagat caaattgttt tttatactct ttaaacccaa tacgaacata
      961 ctctgtgttt gtcttttcat acacatttcc tctttcctgg tatcgccagc catctttcag
     1021 gttaaattcc aggaaccttt tattttcggt tacccgcata atgccacttt tggcaacaat
     1081 aaaattatcc tgcagcgggt tcccctgttc atacacgata acatcccgga tcacactatc
     1141 atttgcctct ttttttccga tcttaatggc aaagccactg atcttatcat aaaaaattcc
     1201 ttctttcaga tcaaaggcag gtttggcata tacaatatca gccagtaatg ttcttgactt
     1261 aagattggca accgggatca catagttgga aaaaacaaaa gcgatcccgc tgataaaaag
     1321 gctgacaaaa aatagtggcc gcataaagcg cagtaaagaa atacctgccg acttgatagc
     1381 caccagttca aaactttcac ccaggttacc aaatgtcatt aaggaagaaa gtagtatggc
     1441 caacggaagt gctaagggca ccagtacagc gctctggtaa agaataaact ccaggataac
     1501 gttagtgccc agtccttttc caacaaagtc atctatatag agccagaaaa actgcatcac
     1561 cagtaccagc aacgtaataa aaaacgttgc gataaaggga ccgataaagg ccttaacaat
     1621 cagtttatca agctttttga acacttatct ttattgtatg aatgcagcaa aaataaggct
     1681 ttcgccagag gagagagaat tggtaatgaa tgcgggttgg attttaacaa agaacaatat
     1741 tttacaaaag atcaaccggc agttggggga gctgcaatca gcccagcagg agtacctgca
     1801 aaatttttcg cagtcattgc cggcggcaat tatgaattca tcaccaaaaa tttcaaaagg
     1861 agaaaactat aaaggccttc cttatcttat acttgattac ccaaaatact ttgatcaatc
     1921 tgctgttttt gccatcagaa caatgttctg gtggggaaat tttttcagta ccacccttca
     1981 tcttgccggt actttcaaag atgagttcca ggaaaaaatt attgctgctt ttccgttgtg
     2041 gcaggaaaac gggttctgct gctgtgtaca tcaggagcag tgggaacatc attttgaacc
     2101 cggaaactac aaaccggtag ctgaactaag caaaacagag ttcatgaatc aaataagaaa
     2161 taagtcattt tgtaaactag caaaaaatta tcccctggat aattgggatg agctacccgg
     2221 acttttgctg aaggattaca tgctactggt tacaataaca ggaaaatgag actaattgcc
     2281 caggcgatga aagagttctt tcacctggta atcccatagc atggtctggt ctttgatttc
     2341 ctttttctct gcaaaatcct ttatgatcag gatcgtttga ttggtgatct ccgatttatc
     2401 aatccggaat tcaaaatatt cttctttggg ggcagtcagc cagtgaaagc ggataaattt
     2461 atcctgctct ttttccagta catcggcttt ttcaaaagat ccattccagc caaaaaagaa
     2521 aataccgtct ctttcatcaa ccttatcagc aaaccactct cccagcccgg ccggggtaga
     2581 gaggaattca tataatatcg atggtgaaca gcgaacgggg aactctaaag tgtataattg
     2641 tttactcatt ttatatttaa aatcattgcg atagctgcaa taatagaaaa taaatcattg
     2701 cggcaaaata ttttacggtt tatttttccc cttgttgcaa accaaaccgg gatttgaaaa
     2761 ataaattgga tatgaattcg ttaataggtt gccaaaattt atttggcctg tattggcaat
     2821 tacattaaat tgccttgaag ttcaatactt tctgaacaac ttagacattg tggtgggtat
     2881 ttttagagta taaggccaaa acttaactta taattcttta acccttaaaa tacaccatta
     2941 aaaccatacc tatgagcatg agacaactca agatcacgaa gtctattacc aatcgtgaat
     3001 cccaaagctt agaaaagtac ttgcaggaga tcggcaaggt cgaactcatc acccccgaag
     3061 aagaagtcaa attagccacc cttattaaac aaggatgcca gaaatcattg gacagactaa
     3121 ccaaggccaa ccttcgtttt gtggtatctg ttgccaaaca gtaccaaaac cagggtttat
     3181 cgctgccaga cctgatcaat gaaggaaatc ttggccttat caaagctgca caacgctttg
     3241 acgagacaag gggcttcaaa ttcatctcct acgcagtatg gtggatccgc cagagtatat
     3301 tacaggcatt ggccgaacaa tcccgtattg ttcgccttcc cctgaataaa gtgggcctga
     3361 ccaaccgcat tcagaaagct ttttcattac tggaacagca atatgaaaga gaaccatctg
     3421 ctgaagaatt ggcagagctg ctggaaatgg atattgaaga ggttacttcc acactgggta
     3481 tcaatggccg tcatgtaagc atggattctc ctttatctga aggtgaagaa aacaccctga
     3541 tcgatgtttt ggaaaatcct aatgctgagc gaacagatgg ggagcttgac cacacacaat
     3601 ctttaaaaac agagattgac aggtctttga aagccctgac cgaaagacaa aaggaagtga
     3661 tctgcttttt ctttggaata ggggtcgatc atcctatgag cctggaagat atcggcgaaa
     3721 gattcaacct gaccagggaa agggtgaggc aaataaagga taaggccatc acaaagctga
     3781 aaacatccaa tcgttgtaaa ctattaagaa cctatttagg cgcatgatat aacttataca
     3841 ctttatcttt gcaattcttt aaaaagtgaa catcagatgt tcactttttc ttttactaac
     3901 gatgtcaccc ggcgccgggc aaagggaggc aaacgaaaac gaatcgaagc aaatgttcaa
     3961 cagtttacag gaaaaattag aatcagcctt taagaacctt aaaggccagg gacgtattac
     4021 cgaacttaat attgccacaa cggtaaaaga aatccgccgg gcattggtgg atgctgatgt
     4081 gaattataaa atcgccaaag agtttacgga caaagtaaaa gacaaggcga ttggcgaaaa
     4141 ggtaatcaat gcaatttctc ccggccagtt gatgaccaag atcgtaaaag atgaactggc
     4201 agaactcatg ggagggcaag aggcctcttt taatgcaaaa gggaatcctg cagtcatcct
     4261 gattgctggc ttacagggta gcggtaagac cacattcagt ggtaaactgg ccaacttcct
     4321 gaaaacaaaa aaagggctct cccctatgct ggttgctgcg gatatctacc gtccggcagc
     4381 aatggaacaa ttaagagtac tgggcgagca aatcggggtt gatgttcaca ttgaactgga
     4441 aaataaagat gctgttgata ttgcccggaa tgcagttaaa gaagcaagaa gtaaaaacaa
     4501 gaatgttgtt attatagaca ctgccggtcg cctggcggtg gatgaagtga tgatggctga
     4561 agtggccaac gtaaagaatg ctgttaaccc gcaagagata ttatttgtgg tggatagtat
     4621 gaccggccag gatgctgtca acactgccaa agcttttaat gaccgattgg attttacagg
     4681 tgttgtactt acaaaattag atggcgacac aaggggtggt gctgccattt ccatcaaata
     4741 caccgtaaac aagcccatca agtttaccag tagcggcgaa aaaatggata cactggatgt
     4801 attctaccct gacaggatgg cccagcgtat cctggggatg ggtgacatta catcattagt
     4861 agaaaaagcc caggaacagt ttgatgaagt agaagccaaa aaactggaag ccaaacttcg
     4921 taagaataaa tttgattttg ccgactttaa gatgcaatta gagcagatca aaaaaatggg
     4981 taacatgaag gatatgctga gcatgatacc cggtatgggc agcaaaatca aagacctgga
     5041 tattaacgac gactccttta aaggcattga agccatgatc aattccatga caccggaaga
     5101 aagggccgat cccgacctga ttgatgggag ccgcaaaaaa aggattgcca aaggcgccgg
     5161 aaaagacata ctggaagtca acaacttcat gaaacagttt gaacaaatga ggggcatgat
     5221 gaaaaacatg aacaaaatgg gagctttcgg caaaatgatg cccggcatta aaaagtagac
     5281 ggaaagttgg gaagtcagaa agtccgcaag aaagttcacc tgatcagata gattctttct
     5341 ttttactaaa ggcgtgatat tttttaaaca gcaacctcct tcttccggac tttctgactt
     5401 acagactaat tgacttttcc tttgcgatct ccccttccca ccttatattt gccgtccggt
     5461 ttccaccgga actaacaatt attgtttaac gctgttaaat ttctatttat gtcagcaaaa
     5521 atccgtcttc agagacacgg gtcaaaaaag agaccctttt atttcatcgt aattgccgat
     5581 tcccgggcac cccgtgatgg caaattcatc caaaaattag gtacttacaa tcccctgacc
     5641 gttccggcta caattcagtt ggatcgtcag aaagcattgg agtggctgaa caaaggtgct
     5701 acaccaacag acactgttcg ccgtatcctc agctttaaag gtgtattata ccttaagcac
     5761 ctgctccgtg gtgtgggcct gggcttgttt gatgatgcaa ctgcgatgga aaaattcacc
     5821 aagtggagtg ccgagcatga agcacaggtg aaaaaacgcc aggaagatgc ctaccgcaac
     5881 aaaagagcca gacgtgccgt tcccggtcgc agaccggaaa gaaggacaga aggtgaaggt
     5941 ggtagtgaag gataaattat ccgcatcctg aaagttcgta atcccgtctt gttgtaaatg
     6001 agacgggatt ttttgctaca gggtattacc aggttaacac agattatatt tgcttaaatg
     6061 actgaatatt ttaatatcgg gaaattagtg gccgtacatg gcctgaaagg cgaactgctg
     6121 ctcaaacatg aactgggaaa gaaaacatca ttgaaaggac tgcaagcgat tttcattgag
     6181 gaaaagaaaa actcgtttat tccctatttt atagaagcaa caaaaataaa atccgaagat
     6241 gaagtatata ttaaactgga aggcgtaaat acaagagaag tggcaatcaa actcacccaa
     6301 aagcaagcct ggttacccga acctgatttt aaaaaatttg cggcaaaatc ggcacctgca
     6361 agcctcctgg gctatactat catacataag aatgaatcac tcggagaaat acttgaactg
     6421 atcgaacaac cacaccagct actctgcagg ttagacataa aagggaaaga agtgctgatc
     6481 ccattacatg aagaaacatt gcaaaaagta aatcacaaga agaaagaagt gttagtagaa
     6541 ttgccggacg ggttactgga aatttatctt acataacaaa tttgtgttaa ccagtgttca
     6601 ttacccgttt tcgggcggga ccaattatgg aagaagcaaa acatcatatt gcccattttg
     6661 atctcgactc tttttttgtt tctgtagaaa taagaaataa tccatcctta aaaggcaagc
     6721 ccgttttagt aggcggttat gaacggggag ttgtagctgc gtgcagctac gaagcaagaa
     6781 aattcggaat tcattcggcc atgccaatga agaaagcaat gcaactctgc ccgcaggcca
     6841 ttatcaccaa cgccagccgt gaccagtaca gcaaatactc aagatgggta acagagatca
     6901 tcgcatccaa agttccgctg tttgagaaag caagtattga tgagttttat atcgacctga
     6961 cgggtatgga taaatttttt ggggtaagtc aatatgcaaa agatttgcgt agtcttatta
     7021 caaatgaaac aggtcttccc atatcctgtg gattagcttc agcaagattc attgctaaaa
     7081 tggccaccaa tgaagccaaa cccaatggct tcttagaaat tccgcatgga aaagaaaaag
     7141 aatttctgtg gccgttgggc atagaaaaaa taaacggggt gggtaagcaa actgaacagc
     7201 aattaaaaag ttatggcatt tacaccattg aagacattgc caaaacacca attgaacacc
     7261 tggagaagat tgccggcaaa tggggcgaag cgctatggca taaagcacat ggcattggca
     7321 gtacagcaat aacaacggat tgggaacaaa aaagtatgag ccatgaaaat acttttgata
     7381 cagactatac ggatgttagt ttcctgcata aagaattagt acgattaact gagaagacag
     7441 cttattcttt acgggaagat gaaaaaatga ccggttgtat tacagtcaaa atccggtatt
     7501 cagattttga aactacttca aaacaggaaa cagttgatta tactgccctt gacgatgtat
     7561 tgataggaaa agtaaaagac ttgtttaata aatcctacca aaaagacaga ccggtgcgtt
     7621 tactcggtgt ccggttcagt catatgatcc caattaccat gcagatgagc ctgtttgaga
     7681 acaatgtcga aaaactcaac ctctacaaag cggtagatga gattaaagac aggtttggca
     7741 gtaagttgat atccaaagca gttacttctc aggtaaaaga caattccaac gaattcacta
     7801 caaggcattc taaaaagaaa aagcccggct gatatcagga ttgtttaaaa tgccctgctg
     7861 gatccatgat tccaaatagc aattggcgca caacccgtag ccccgcaatg tgtacaatag
     7921 cttccttttc tggtgttatt attccatgac tttggattac ccccgcaact tttgcaagga
     7981 ccaatttgcg taacaatata tgcaccgctt acaccatcaa tcattctaac caggtccttt
     8041 acttttatat tggtggtttc ataagttgac gagctgatgc cgaattcatt tctaccggaa
     8101 gaataagagt agctgaattt tgcaaaaata gttttaaggg taatttcgtc attaacacta
     8161 tttataacaa tagtacccgg agtgaaataa catgtttttt gtgaacccag gtataccatt
     8221 tggccttgta aatatttcgc acttcccctt agtattgcaa gctcatcctt tggcttagcc
     8281 ggtgaaacta ttgttggtgg tgttttattt ccaccattgc tattatttac tttgttacta
     8341 ctaatcgttt taccccatcc ataacctggt tggggtacac cagttggata agcaggttta
     8401 gctgtgttat tctgcgttgc ctgaaattca tcgacggcat atttattcaa cagtccaact
     8461 gcatttcttt gctcggcagt caacactgca cttgctttca tgcataattt tacatcatca
     8521 aaattaattc tccgcgacat gagcacctgg taatagccat gaaagtcgat atctatcatc
     8581 tgcaaaaact tttggccaag taaaatctcc agtttgtcag cggagaaatt caatgctttt
     8641 aacgccagat agtaatcgat aatagcatcg gcccgttcct ttgaatttcc ggctttggcc
     8701 agtgcctttt gataattaga ctcctgaggc tcgggtgaaa gtgtaattgc tgataccgat
     8761 ccctgaaaga ttttcaaata atcaatatca actaattttt tgttattcca ctgaaagcca
     8821 ataccggtat gactactatt aaatatctca tattttgcct tatgtacttt actgccattt
     8881 acaaaaaagg aaattgtatc cttccttttt tcaacacgga gcacattggc tttgttaata
     8941 ccggcattta ttgaagtgga agcaatagtt ccggcttttc catcccattg actgttgacg
     9001 agttttccat attgaaactt gccttccgga taaataccga aagcataaaa attcttaccg
     9061 ttatcgccaa taccccaaag caaccagtaa ctgtccaaag gatttcctgt ttctgtactg
     9121 ttcaataaca tcttcgtttc gatggcaaaa tcttttgatt gatcggaagc taggcccggt
     9181 acgcttaacc aggatgcata attattactt tgttttacgg tcacccggta attgccttta
     9241 tgtaattgtg cagccgagcc ttcattatcc cataccgccc atttgttgtt gttgttgtca
     9301 aagttttcgt caagaatcag agatagctgt gaattattct tattgtcagt tttagctgtc
     9361 tctttattgg tgaccggaag cggttcataa tactctatct ctcttgtttc tctttttgaa
     9421 ttggaaatcc agttaccggc tgcatccaat ttataagtga gttctctcgg cctgacggaa
     9481 aaattagtag cgccgatgtt ttgctctttt ataatattct gtttgttatc aaaatatttt
     9541 atactggttg agttttcaaa ccttccgttg ccactataat cagtcctggt ctgaatcttt
     9601 atcaatggaa gaccatcctg tattttgtat tcataggcga tttcagccag cagtaccctc
     9661 gctttttggg tatatgtttt actgctgatt atttgcccct cgttgttata ctggtgaatt
     9721 atgtaataat cgaaagcaga gggattataa gcgctgtcca atacaaaaag gttatcctgg
     9781 tagtgatata ctctttttga ataaaaaatt gtccctgcct ggtttgctat ataatagttg
     9841 gtaacatcgc cgttgctgtt gtatacatat ttatagaaca ccttttcccc cgttgcatat
     9901 tctgtttttt gtaaaattga tcgggttgtt gagtcataac taaaattatc gaatacggct
     9961 gatcgttcgc tgccgacaag cttttttgta agtcttcccc tgatatcata accttcctgg
    10021 ctgataactt ttcctgtact gtcttttaat gtaatttttt taaccttccc ttttaagccc
    10081 tcatgttgta aaagatagcg gatggcaaca gggttttcgg aacgaatcag aggtagctga
    10141 tctattttat cagtccggct gcttttttca ttgcttgctg tatactgcgc aattgcacaa
    10201 attgtgctaa agaaaaacaa acctgtcaat tgtataattt gcttcatttt ttttctttta
    10261 aaactaaaaa aaccgacttt actgtatacc agttagcgga ggtctacaaa aatattttcc
    10321 ttacaacata attacctgtt atggccagga caatcaaaaa tgcaacaaat aacagagccg
    10381 taaaccagtc aacactaaat gcatcgtaaa tcaatcgctt gtcacttacc aggaaaaggg
    10441 gaagcccggc taacacaacg gaagtaccaa ggctgaatgc cgtagctgta gccggatgaa
    10501 aaggcaaact acggcccatc attaatagtg taaccggtac agagctttgg agaagagcaa
    10561 tccctgtaca caacatgtat atattagttt ttccaaactg caaaagaacc aatgcggcta
    10621 acagcgttat gtaaataaat tttctgatcc ctaccctgtc agccacaaag ccaccaatga
    10681 tctttccaaa aaatgcactt accccgatat acaacaggcc atcctcgtaa ttttcagcta
    10741 catagtttac cacatcaaat aaaaaagaac ggaagcacat aaaaagcagt aataacagca
    10801 ttacaagatc atgactatcc aattcacttt cctttttctc cggggtctga taaaccggca
    10861 aaggctgtct gaaaatggta attgttacaa gcattgcagc aagtactaag accaggggca
    10921 gcaaataacc agcttcaccc aataaaccac caatggtaag acccaatacg ccgggagcag
    10981 taaataatcc caatgggccg gatttatttt catgcacctg taaacaaatt gttccgccgg
    11041 ttacatgtac aaatgccgaa gccactcctg aacatatgat agccatctcg gtagaaacaa
    11101 aaaagaaaag taaagaaagg ggtaaaagtg agaccgatat tttggcaaac aaagcaatgt
    11161 ccttttgctt gtccagccaa aaaccgacag gtaactgacc accgaaaccc agtattgcat
    11221 aaatactgat gaaaagaaaa ctgtcgctgt atccatgtga aagcgtatac ccagccagca
    11281 agaaccctgc tgccaggtca ttaatgccat gcagcaatcc ccagtaaacg ggttgtaaac
    11341 tccggttatg agataaattc ataaactata attattaatc agcaagccag ctccatagct
    11401 tatcccattg gcaataaatg cagctgcaaa cgatctcccc caactgcttt tcattaataa
    11461 cttataacct gccatttcgg ccagcgccac acccagttcc ataagagata gtgaaatatc
    11521 agttgttaaa agcaaaaagt gtaataaggg ccaggtgaac aggtttaaca aaacaaacgg
    11581 aacaagaatt tctttccatt gtttataata aaacagcagt attatcggaa attctatcag
    11641 tagagtcagg gcaaaaaacc accaggcatt catcagcatt cttgtttatt tttccttctc
    11701 ttccagataa aaaaagccac aatacccacc attgctgcca gggcgataat tatcagccaa
    11761 atattagttt tagctttctc agccagtgtt ggctctggtg gtacatccaa taaaaatctg
    11821 aacatagtat atgatttaaa taattccaat catccgtttc ctataaagaa aaagagaata
    11881 gcaattgtat taactatgaa agctgccggc actgctttcc accagttgtt ttgcaccaat
    11941 aagtttaata caacaatatc agccaatatc attcccgcat aaacaaatgt aaaatcaagg
    12001 gaggtttcat gatacaaaat ggtaattaag ggtaccacag ccaaactggt gaataaagct
    12061 acaagcaatg atgccttcca ctgccgcagg taaaaaggca attgaaaaat tcccttaatt
    12121 aaaaagaatc ctataagtgc gatgatcagt ggcataatca agaatggttt aagccggttt
    12181 tatcgttttt cgtttcagga aaagtaatat caacagcata aggccggcta aagagcacag
    12241 gataaatatc caccggttta cccggccatt actacccgaa tcatcatcag caatcactgc
    12301 ataatcaaaa taggctttcg actttttcat tttgtattgt agcttgctat ctttcgcacc
    12361 ggtaataacc atttgatagt tccacttttt catatacacc gtgaatgtat ctgtaaccgt
    12421 gccggctgaa tccgacttag ccaaagcaaa atgcaggtag cgttcagatt catcccatct
    12481 ttttcctcca tcctggatta ctattttgta attatcatca atagtatccc ggcgactgat
    12541 gaaagggttc tttaacagtg tatcttcttc gtggtaagaa tattgtacta gtaccagttt
    12601 ataaccggcc aggttcccgg taccttcaaa agttatctgc gccggggcag tacggcggcg
    12661 aatagaaaat cctgcatcag ccattgctgc caggctggtt aaaaccagca atgctgtaaa
    12721 aaaagctctt actcttttca taccattaaa tgtagattat ttcattgaag cctgaatacc
    12781 cgccacaggg tataagtaaa atcaggaaca gccggtaaaa acctcatacg gggataaaat
    12841 cttcctgctt atcttcgcca accaatttaa taataactca attactatgt cacaagtttg
    12901 gaaagattac caggaaaaga ataaggaccg ttacctgaat gaaatgcttg acctgctccg
    12961 gatcccttct gtaagtgcta aaagcgaaca caaagacgat atgctgaaat gcgcagaagc
    13021 cgtgaagaaa agtttattgg aagctggctg cgacaaagct gaagtaatgg cgaccgatgg
    13081 ccaccctgcc gtttatggtg aaaaaataat tgacccgtca aaacccacgg ttcttgttta
    13141 tggacactat gatgtgcagc caccggaacc tttagaactc tggaccaatc caccctttga
    13201 acctaccata atagatggta aaatatttgc ccggggctca gctgacgaca aaggccagtt
    13261 ctatatgcat gtaaaggcat tggaaacttt agtgaagacc aagacaatga ccaccaatat
    13321 caaattcctg attgaaggag aagaagaagt ggggtcgccg aatttaggaa aatttgtggc
    13381 tgctcataaa gacatgctga aggctgatgt aattctgatc agtgatagtt ccatgctgag
    13441 catggagcat ccatccctgg ataccggtgt ccgtggttta agctatatac aggtagaagt
    13501 aacaggtgcc aacagggatc tccatagtgg tacctatggt ggagccgtgg caaaccccat
    13561 caccatactg gctcaaatga tagcttcctg tcatgatgaa aataatcata tcactatccc
    13621 cggattttat gatgacgtgg tgattgccag tcctgaagaa agagcattga tcaatatggc
    13681 tccgtatgat gaaaaagaat atatggatga actgggtgta aaagaattat ggggtgaaaa
    13741 aggatacagc acctacgaaa gaacaggtat ccgtccaacc attgaaataa atggcatctg
    13801 gggcggttat accggtgaag gggcaaaaac ggtgctgcct tcaaaagcat atgcgaaaat
    13861 ttctgcaaga ctggttccca atcaatcttc tgataagatc acagaaaaat tattggctta
    13921 cttcaagtca attgccccaa aatcagtaga agtaaaagct gaactgcatc atggtggcga
    13981 gccatatatg acaccgattg acagcaaagg ttataaagca gcagcaaaag cagtagaaac
    14041 tacttttgga aaagaaccga tccctgtccg tggcggtggc agtattccta tctgttctat
    14101 tctggaaaaa gaactaggca ttaaaataat tttcatgggc tttggccttg ataatgacaa
    14161 cctgcatagc ccgaatgaga agtacaatat tgagaattat tataaaggaa tagagacaat
    14221 cccgtatttc cataagtatt ttgctgaaat gaattaatga ctcattgaag aaattcaaat
    14281 cccaatcatc cggttgggat tttttatttt tgaatatgcc tgttaaagaa aaactccgcc
    14341 ttgataaata tctctgggct atccgcttgt tcaaaacaag gtcaatggca gctaccgcct
    14401 gtgatagtgg aaaggttaaa tttgacggct cccaggccaa agcttcccgg aatgtgagta
    14461 tcggcgatga gtatgaagta aaaacggaag ctaaacggtg gaggataaaa gttacaggac
    14521 ttctggagaa aagagtagcc gctgccgaag ccattaaaaa ctacattgat attacacctg
    14581 aagaggaaat agaacgaata agattccagg cagccagttt tcatactggt aaaaggcaaa
    14641 gtaaaatcgg aaggcctacg aaaaaagaaa gaagagacct ggatgaattt ttagaataag
    14701 aaaacttcct tttttaagca atagtggaga tagttttgat aaaatcagga tttttttcat
    14761 ccaacagatc accaagcttt ttggcagaaa attcagccag cataccatcc cgcagtttca
    14821 acatctgaaa atggagcgga cagggattct tgctgttaca ttttcgcaaa ctcaatacgc
    14881 agatattgaa ctgttccact ccatcggtaa tctttaccaa ttgtataagt tttgtcttta
    14941 atgtgacttc attcaggtaa aagccaccat aagggccctt ggttgatgac agaattcctt
    15001 ctttaaccat cattttcatg atcttggcga gaaaatgccg gggaacggat aatttttccg
    15061 caatctcatc cagctgcact tttcttcctt ctttgtctac taaagctata tacaacactc
    15121 cccgaagggc ataaccaaaa gattttgaaa ggaacattat tataagtcta tgatcgacaa
    15181 aagtacctgc aggtattttt tgacggggtg actaatatca cagagaacga tgatgaatat
    15241 cagacacctg caaaactact caaaatacat ttgccccata aaagacattg atatcttttt
    15301 aatattaatt ttgaaaggcc cacgatcttt ttattattca ttaaaatcat tccaacatga
    15361 aacgcaacat tcttggtact gttttattat ctgccagtat tttctttttt tcctgtaacg
    15421 aggggggagt aaaagatgaa cccaaaccct tagatgtaca ggagctcacc aaagacaaac
    15481 cggaaacaca cggttctgaa gtaaaagaag gagacattac tttaagtact ccgcttgacc
    15541 aggctatggt agctgccggc aaaggaactt atgaattgaa atgccaaagt tgccataggc
    15601 ttacagaaga aaaactggta gggccgggat ggaaaggtgt tactcaaaaa agacagcccg
    15661 tctggatcat gaatatgatc actaatgtag acatgatgct ggaaaaagat cctgaggctc
    15721 aaaagttatt agagctctgc cttgtacgta tgcccaatca aaatatcact acagatgaag
    15781 cccgtaaagt agttgaattc atgcgtagca atgatggtga gaaataagtt tacctgctga
    15841 atcaaaaccc cgaatatatt tcctgatttt tcttaactgc taaatattat atatgaaaca
    15901 ctcattccta aaaacgtttt ttgcattttc tttgctgctg atcggcctgg tctggctgca
    15961 gggctgtaag cctaaaggca ctcaatcagc tgccggaggt gatgctgcca aagcatatgt
    16021 tgccccgggc aaatatgacg aattctacaa ttttgtttcc ggtggattta gcggacagat
    16081 gtccgtttat ggtttaccat ccggacgctt atttcgtgta atacctgttt tttctgttga
    16141 tccggaaaaa ggctggggct atagcgaaga gacaaagcct atgttgaaca cttcgcatgg
    16201 atttgtaccc tgggatgacc tgcaccatat tgccttatct gtaaccggca gtgaacatga
    16261 tggtcgttgg gtatttggta atgccaataa tacaccccgt attgcccgga ttgatctgaa
    16321 aactttccgt accgctgaaa ttattgaaat accaaacagc gccggtaacc actcctctcc
    16381 ttttatcact gaaaattctg aatatgtagt agcaggaacc aggttcagtg tgcctcttgg
    16441 tgataaactg gatgtaccta tcaatactta caaagaaaat ttcaaaggta ctatcagttt
    16501 tataggggtt gacaaaacat ccggagaaat gaatgttgcg ttccagatat taacccctgg
    16561 tatcaacttc gacctggccc gtgccggtaa aggaaaatca gccggctggt tcttcttcag
    16621 ttgttataat tctgaaaaag ccaatacact gctggaagta aatgcttctc aaaaagataa
    16681 ggacttcatc atggcagtaa actggaaaaa agcagaggaa tatgctaaag ccggtaaagg
    16741 agttaaaaaa gctgttcgct atgcgcataa taaatatgac gataacacac attctgctac
    16801 ttctaccatc atgaatgaag tactggtgct tgatcccaaa gattgcccgg atatgattta
    16861 ctttatgcca tgtccgaaat caccacacgg atgtgatact gatcctaccg gcgaatacat
    16921 tgtgggaagt ggtaaactgg ctgccgtaat acctgttttt tcatttacca agatccagga
    16981 agcgattgcc aagaaaaatt atgatggtga atttgacggt attcctgttt tgaaatatga
    17041 agctgtttta catggtgagg tttcaaaacc aggtcttgga ccattacaca cagagtttga
    17101 tgccaacggg aatgcatata catctttctt cgtatcatcc gaaattgtaa agtggagtgt
    17161 aaaagatctt aaagtactgg accgtgtacc tacttattat tctatcggac acttatcagt
    17221 acctggtggt ccaactaaaa aaccctgggg caaatatgtg attgcgtata ataagatcac
    17281 caaggaccgt tatcttccta ccggacctga actgacccaa agtgcacagt tgtatgatat
    17341 cagtggagat aaaatgaaat tgatccttga tttccccact atcggtgaac cacactatgc
    17401 agaagcaatt cctgctgata tgatcattaa aaacagccgg aaaatattca agctggaaga
    17461 aaataaacac ccttatgttg caatgggaga aggtaaaaca aaagtagagc gaaaaggaaa
    17521 tgaagtgcat gtttatatga cagctatccg ttcccatatc actcctgata atatagaggg
    17581 tgttaaactt ggagagactg tttatttcca cgtaaccaac cttgaacagg actgggatgt
    17641 gccacacgga tttgcagtaa aaggaatcag taatgctgaa ctgcttatta tgccgggaga
    17701 aacacaaaca ttaaaatgga cacctgaaaa agccggcgtt tatccaatgt attgtactga
    17761 cttctgctct gccctgcacc aggaaatgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn ctgcaggcgg acaggaagct gtacaggatg atgaatcaca aaaagatgtt
    17941 gtaaaagttg ctgtcggatc agcagatcat tcaacattgg tagctgcatt aaagcaggct
    18001 gagctggtta cctctctttc caatgcagga ccttttacag tatttgcacc aacgaatgcc
    18061 gcttttgaca agttacccgc aggtactgta gacggactga tgaaagatga taagaaagcc
    18121 gatctgcaaa acattttgca ataccatgtg gccgttgctg tatataaaac agaaaatatg
    18181 aaagatggcc aaataattgg ccaggccaat ggagataata ttactctcag tgtgaaggat
    18241 ggtaaggtga tgataaatgg cagtgcaaat attgtggctt ctattcctgc ctccaatggc
    18301 atcatacatg taattgatgc agttttgctg ccaccagcca aaaaatagtc aaacgtcgtt
    18361 gcgtttttca ttgtacactt tcacttgctt ccggggtaca ataccccggg agctttttaa
    18421 gacaatcgta tgaataacaa gctcagtgta acatcccgga ttttagtggc ctttgcctct
    18481 ggtgccctgg tgaccgtatt ctttttaccc gcctggcgga ttgacctgtt tgcaccgcag
    18541 tacccggaag ggctgatgat gaatatctgg ataaatggat taaccggtga tgtagacatt
    18601 attaacggtt taaatcatta tatcgggatg aaacatatca gtgttgatat gttcccggag
    18661 ttcaagtttc ttccctatgt agtaggtttt tatatgctcc tgggaattgt tatcgctata
    18721 acaggcagca ggaaattctt attactgtat cttatcctaa ttgttatcgg cggcgcttta
    18781 gccatgtatg atttttacca atggggatac gattatggcc acaatcttga tcccagtgcc
    18841 gccatccagg tacccggact atcctaccaa cctcccctct tcgggcataa aagattatta
    18901 aattttgacg cctattcatt tcctgatgtt ggtggatgga ttgtgatagc tgcctctgcc
    18961 cttgctttcg gagtttggtt ggctgaatgg tatcatcaac gtaaactgaa taaaaaatga
    19021 gaagttcatt ctttgttgca gtggcagcat ttgtattgat cctttcttcc tgctctgcac
    19081 aacccgaacc ctttaaatat ggccaggaca tttgttatac ctgtaaaatg ggaatagttg
    19141 atccaaaatt tggttgccag cttattacaa ccaaagggaa aatttttaaa tttgatgatg
    19201 ttgtttgcct gtcccgattc atcaaatcag gcactgtaac tgaaaaagac atccggcaaa
    19261 ctgttgtaat caattttgaa aaagagaacg acttcctgga tataaacaaa acattctttg
    19321 ttatggcacc agcaataaaa agtcccatgg gtagtcatgc agcagctttc tccggtaaag
    19381 aggcggctga gaaatttaat gccggcaaaa ctggtcaaat cctgaactgg aaagacttat
    19441 caggaaaact agaatgacca atgcagtatt tttttaccat agtactgtct tttttgctta
    19501 ctcctgtttt cagcaggaca attattgttg gaccaactca acctatacgt tcgctgaaaa
    19561 aagcaattga aaccgccgac gaaaaggaca gcatcattct gctaaaagga acttataaag
    19621 aaggaagcat cattctaaca aaatcactca ccattatcgg gcagaataat ccggttttag
    19681 aaggtgaaaa caaatacgag atactgctta tcagcggtaa aaatattatt gtaaaaggca
    19741 ttgagtttcg caactccggt tattcagcaa tggatgatta tgctgccatt aaattggttg
    19801 attgcagcaa tgtgacgata gaaaataata gtatccggtt tgcttacttt gccattcaca
    19861 tttccaacac caccagggcc attatccgta ataaccgtat taacgggatt ccaacctccg
    19921 aacaactgac aggtaatggt attcaccttt ggaaatccag tcatgcgata atagagaata
    19981 actatataca gggccataga gatggaattt attttgagtt tgttacagaa tcacagataa
    20041 aaaataacct cagtgaaaaa aacatccgtt atggactgca ttttatgttc tccaatgacg
    20101 ataattacca gaacaataca ttcagaaaca acggtgccgg tgtagcagtg atgtactcga
    20161 aaagagtaag aatggaaaaa aatcattttg aagaaaactg gggagcctca gcatatggca
    20221 tcctgttgaa agatatcaca gacagtcaca ttcaaaacaa taccttcttt aaaaatacgg
    20281 tggggattta tatggaagga agcagccgta tagagataca aaagaatttt tttaaaaata
    20341 atggctgggc catcaaagta caagccagtt gtgatgataa tgttttccac cataataatt
    20401 tctccggcaa cagctttgat gttggtacaa atggaacaat ggtgctgaac aagtttaata
    20461 ataattactg ggataaatat gaaggctatg atatgaataa agatggcata ggagatattc
    20521 cctaccatcc tgtgagcatg tattcgatga tagtagaaca aaatccaacg accttaatgc
    20581 tattcaggag ttttatggta tcgctgctgg acaaagctga aaaagctatc ccgagcctta
    20641 cccccgaaaa tctttttgat gatgcaccca tgatgaaaat gatgaaaata tgattcgtat
    20701 tgagcaggta aataaaaaat tcagaaaact ccaggccctg gacaatatat cggcaaactt
    20761 cgatagaggc caggtcattt ccctgatcgg tccgaatgga tccggtaaaa caacactgat
    20821 aaagtctatt cttggtatgg taaggccaga cagcggaaag atctttgtaa aagatcagcc
    20881 tgtgaatggc gaccctgcat accggaaaga cataggctat atgccgcaga tcggccgtta
    20941 tcctgataat atgaaagtgg ggcagctttt tgaaatgatg aagaagatcc gcaatgcgga
    21001 tgatagtaaa acagacaatg atctttttga gcaatttagt attccctcca tttttgaaaa
    21061 gccaatgcgg acactgagtg ggggtaccag gcaaaaagta agtgctgccc ttgcattttt
    21121 atttaatccc cttattctta ttctcgatga gcctacagcc ggacttgatc ccctttcctc
    21181 tgaaatacta aaagaaaaaa ttattagcga aaaacaaaag aacaaactta ttctgatcac
    21241 ctcacatgtg ctgagtgacc ttgaagagct gaccacacat attatgtacc tgcaggaagg
    21301 taaaatgcag tttctcaaag acctggaaac attgcaggaa gaaacgggtg aaataaaact
    21361 tagcaaagcc attgccagag ttatgaaagg tcacgaaaca gacagtaaat ggatcgatca
    21421 gatgttttcc gtcaaaaaaa aaatatgacc aggataatca attaaatcaa aatgctaaaa
    21481 gtttcccgat acgttttata tgatatattc aggaataaga tagtcattgc ctacacacta
    21541 ttcctgttta ttgtttcttt cagcatgttc cgtatggaag agaacagcag caaagcaatg
    21601 ctcagtttac tgagtattat tttaattgtt ttgccattgg tcagccttgt attcagtact
    21661 attcactatt ataattcata tgagtttatt gaactcatgc tttcacaacc gctgagccgg
    21721 acaagaatat tattaagtga atttggcggc gtttgtattt ccttgttaag cgccttttgg
    21781 attggtgtag gtgtgccggt attgattttt tctgctgacg aaaccggttt tgcactctta
    21841 ttcaccggta ctgcattgac aattgttttt accagtatcg ctttcctggc atcagtcaga
    21901 tcaaaggaca aagcaagagg aattggcttt gcgttactat tatggttcta ttttgccctg
    21961 atttatgatg ggctggtttt gctgatcctt tttgctttca gtgattaccc gatggaaaaa
    22021 tttaccctct tactttcagc ccttaacccg attgacctcg gaaggatttt cataatgctg
    22081 aaaatggaca tcagtgcttt gatgggatat accggtgccc tctataaaga tttttttgga
    22141 agtggcaaag gtgttctgtt tacaacagtc attatgctgt tgtgggctat tatcccatta
    22201 tggctggcag taagaagctt caggaaaaaa gacatgtaaa taatttatca attttaaaat
    22261 aattatatca tgaatgaatt gatgaccaaa agccttgcac agattgtaac gggtaatcac
    22321 cggacagctg cgatatttga aaagtatcac cttgattttt gctgtaaagg aaaacgttca
    22381 ttggagcagg cctgtaatga gcaaaacatt gctacaaacg aactgttagc agaactggag
    22441 tctatttcgt ctgctcctgt tacaggtaca gcaggtttcc cttttgatca gcttacgctg
    22501 acacaactaa gtaactatat tgtatctact caccatgcct atgtaaaagg ggaactgcct
    22561 cagctctttt cttatattga aaaagtggct gctaaacatg gtgaacgaca cccggaatta
    22621 tataaaatat tcgaattggt ggtcgccatt aaagaagaaa tggaactgca tatgaggaaa
    22681 gaagagcaga tactctttcc ccgcatccag gaaattgaaa acatagctac acaagacaaa
    22741 gaaagtccgc aattgaatat taactacctg caatcaccaa tcagtgtaat ggaacaggaa
    22801 catgatcatg ccggtgcatt aacagccgag atcaggcaat taacttctaa ttatactgca
    22861 ccaccggatg cctgtactac atataaattg ttatttgcat ccttgcaggc ctttgaaaca
    22921 gacctgcatc agcatgttca cctggaaaac aatatacttt ttcctaaagc catagaatta
    22981 ttccagcatt taaatgaagt aagaatgaat taggaacaac cctgtaataa ggaaattatc
    23041 aaacaaatgt tttagagagg agagctctct tgattattga ttgctgacga aaatcatata
    23101 gccttcccat caacatcatt gtttttccca ccgccggcat ttgaaattag cagagtaaac
    23161 aatattgttc acccctaaaa tgtttaatat ggaaacaaaa gcagttgaca agccgagaac
    23221 ttcctggcca actttgtttg acactgggtg gatggacaaa gtttttaatg ccccaattga
    23281 cgaatttttc aatatcggca aagtaattaa tgtaccggca gtgaatgtaa ctgaaacaga
    23341 caaagaattc aagttgaaca ttgctgctcc cggtcttgaa aaaaaagatt ttaaagttga
    23401 agctatcgat gacatgctga ctatcagcgc agaacatgaa agagaagaaa aagaagaaaa
    23461 aaatggacgt tttaaccgcc gtgaatataa ctacagcagc tggagcagaa gttttaccct
    23521 tcctgaaaac tgtgatagcg gaaaaattga tgctgaatac aaaaatgggg aattgaaaat
    23581 tttcattcca aaactggaag tgaaagaacc caaaaaagtg aaaagtatct ctgtgaacta
    23641 acaggatgtg cccgtaaggg tggcccccgc caatatctga aaggcggggg ttttctattt
    23701 atacctaaag aaataagact tttatactct gcagcatttt atctacttcc ggtattgatg
    23761 aaagcttaat agatattgag gtctgtataa ctataccggt ttagtaaagc aaatgtttca
    23821 gtttctctac attgctgatg ctgatcttcc cgtcctttat ttcaatcagt ttctccgatt
    23881 taaaatcact gagtgtacgt ataagtgatt ctgtagcagt acctacataa tgtgcaatat
    23941 cttcccggga gatctctatt ggcttattct caccacttgt attgaatttg ccatgtatat
    24001 ccaccaatgc tttggcaacc cttttgcgca atgagctgta cgcaaggctt agcagccgct
    24061 cttccttttc tttcacattt tgtgtaatga tcctgataaa ttttgaagct actgtaatgt
    24121 taccctcaat catctgctga aaatcttcac ggggcacctg tacgatttct gtatcctcta
    24181 aggctacagc agaatcatca tagttttttt cttcaattaa cgcaggataa ccgacaaaat
    24241 caccatcgct gtataaatcc gtgatgtatt cctttccgtc ttcgtgggtt ttgaaccctt
    24301 ttacctttcc ctttacaaca taatacagga atctcggcct tttgccttcg gcataaatga
    24361 cctgcttttt tgtataaatc tctgaattgt aattatccgg gttgaactga agcataccgt
    24421 tttcccgcag gtcattggcc aattcactca gtcctttgcc gctggcatcg tacttctttt
    24481 gtaatacatc gtattttttt agccttgatt caatggcatt cattaactca atttcttcaa
    24541 aaggcttggt cacatagtca tcggcaccca tttccatgcc tttgcgcaga tcacttcttt
    24601 ctgccttggc cgttaaaaat atcagcggaa tgttttcagt ttctggattt ttcctcaata
    24661 aatgcaggac tccataacca tccagctccg gcatcataat atcacagaca atcaggtctg
    24721 gcttagaagc cagggccgct tccacccctc ttttcccatt ttctgcagtc acagtcttat
    24781 acccccccag ggaaagtatt tcggctgtat tttccctgat atcttcgtta tcatctataa
    24841 taaggatagt tttttgagac ataagtggaa tttttgattt aactaagctg caaaataggc
    24901 aaaagcaacg tttgataaaa atcagtttga taagtgaact tgctcatccg gaggctgaga
    24961 ccccccggtt actttagcga cctgatatat ttccggaaaa atggctgcac cagtaattac
    25021 tgataaggag attcattgct atcactgtgg tgaaacctgt ctgaatgaca aaatacgttc
    25081 gacagaaaag gttttttgtt gcgagggatg caaaatggtg taccagatcc tgagccaaag
    25141 tgacctttgt gattactaca acctgaatga gaacccgggc cttagccagc ggattaaagt
    25201 cagaaaagac aaattttctt tcctggacga tgaaaaaatt cagcttcaac taatcagttt
    25261 ccgggatgat aaacaggtac acattacttt ctacctgcca caaatgcatt gcagctcctg
    25321 cctttatctt ttagaaaacc tgcaccggtt agacgggggt gtaatttcca gcaaagtaaa
    25381 ttttacccga aaagaagttg atattgtttt attgaccgac aaaaccagcc tgcgaaaagt
    25441 agcggaaaca cttaccagta ttggctatga accttatatc agcctgaacg acctgaaaga
    25501 aaaaagaccg gttattgaca aaagcatggt ataccagatc ggtgttgcgg gattcgcatt
    25561 cggtaatatt atgttgctga gttttcctga ataccttggt attgatgcca ctgaaacgag
    25621 cctgcggtct gtcttccggt ggatcagttt cgggttagct atcccggttt tattatattc
    25681 tgctctccca ttttatcaat cttcctggaa aagcataaag cataaatttc tgaatattga
    25741 tgccccgatt gccctggcca tcatcattac ttttatccgt agtgcttatg aagtaatcag
    25801 cggaaccggg ggcggctatt ttgactccat gacaggcatt gtattcttta tgctggcggg
    25861 gcgcatcctg caggataaaa cctaccggca attatccttt gaacgagact attcctctta
    25921 tttccccatt gccatttcag ttttaaagga caataaagaa gttccaacag ctttacccga
    25981 tataaaacca ggcgatacgc tgctcatcca tcatgaggaa ttaattccgg ctgatggaat
    26041 attgacccgg ggaaaagctt ttatagatta tagttttgtt actggtgaat cactacccgt
    26101 attaaaagaa atgggtgaaa tagtttatgc cggcggcaaa caaacagaag gcaatataga
    26161 gatactggtt gtaaaagaag tcacacagag ttatctcacc aaactctgga acagggacga
    26221 gtttaaggag caagaagaga ataagaaggt atcttttgta aatatcctta gccgctattt
    26281 tacttatatc gttttaacca ttgcagctgc aactgcagta tactggcaaa taaacgaccc
    26341 gtcaaggatg tggcctgccg taactgccat ttttatcatt gcctgcccct gtgccttgtt
    26401 cctctccaat acattcacca atggtaacgt actcagaata cttggcagaa atcatctata
    26461 tctccggaat gcacaaacta ttgaagaaat ggccggggct acacatattg tttttgataa
    26521 aaccggtaca ctaacctcca cccaacaaca ggatatcgta tatgaaggac tggcacttac
    26581 agcatatcaa ctgcaccagg tggcagccct ggctgatagt tccaaccacc cactgagtaa
    26641 agccctcgcc aaacatatcg gcaaaacaaa agaaattcat gtagagggct ttaaagaaat
    26701 aaccgggcag ggcattgaag ggttagtcaa taatgattta ttaaaactgg gctcttatga
    26761 gtatataaca ggtgaagtaa acaggcaacc tggtacagct gtatatatta gttttgaagg
    26821 tgaattattt ggatattttt tattcaacaa ccattaccgg gaagctatcc cgtcattgat
    26881 aagcgaactg aaaaaagaat atcatctttc tgtgctttca ggtgacaata cggccgaaaa
    26941 agataccctg caaaaacttc taggtaaaaa agcaacacta ttatttcatc agaaacctga
    27001 agacaagctg caatacattc aatacctgca gaaccaggga gagaaagtga tgatgattgg
    27061 ggatggatta aatgatgccg gtgcgttgaa acagagtgat attggtattg ctgtggcaga
    27121 gcaaacaaat aattttaccc cttccagtga tgctatcatt gaagcaaaac agctttctaa
    27181 gctcacaaaa tttattagcc tgtgccgtgc taacagaaac attgtgatag ccagtttcgt
    27241 gctgtctatt gtctataatg ttatcgggtt attcttcgca gtacagggaa ccctatcacc
    27301 ccttattgct gccatcttaa tgcccagcag ttcgctgagt attttgttac taacatttgg
    27361 cagtagtagc ctgattgcca ggcagatgaa actttaagcc aaaaattcat ttatcaaaga
    27421 attagcctga taaaaatcag ttcgcctctt gatacttgtt atcctttgcc ttggttgtca
    27481 aaaatacttt taccgctata ttatctaaaa aaatgagtgt aattgtcatc ttattgattg
    27541 ccagtatttc tattgccggt cttttcctgt tcgcattttt atggggcgta aaatcnnnnn
    27601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattgc cgctgctgcc attacacttt
    27721 tgatgggtta taccaccagt aaagaatatg cagaactgga atggccaatc gatatcgcta
    27781 ttactatcgt gtgggtaata tttggtatca atatgtttgg caccatcctg aaaagaagag
    27841 aaaaacattt atatgtagcg atctggttct atatagctac ctgggttacc gtggccatgc
    27901 tccatattgt gaactcaatt gctattccgg tttcatttat gaaaagttat agctggtatg
    27961 ccggtgtaca ggatgcattg gtacaatggt ggtatggtca taatgcggta gcctttttcc
    28021 ttaccactcc tattttagga ttgatgtatt actttctgcc taaagcagct aaccgcccgg
    28081 tttattctta ccgcctttca attattcact tctgggcgct gatatttatc tatatctggg
    28141 ctggcccgca ccacttactt tattctgctt tgcctgattg ggcgcagtca ctgggtgtag
    28201 ttttttcaat catgctgatt gccccaagct ggggaggtat gctgaatggt ttgtttaccc
    28261 ttcgtggagc atgggataaa gtaagagaag atcctgtttt aaaatttatg gtggtggcta
    28321 ttacctgtta tggtatgagc acattcgagg gacccatgct cagtctgaaa agcattaatg
    28381 caatttctca ctttactgac ttgaccattg cccacgtaca cataggtgga ttgggatgga
    28441 atggatttct tgcctttgct atcctttact acatggtacc aaaaatgtgg ggtaccactt
    28501 tatactcaaa aaaattagcc accaatcact tctggttggg tactattggt atcatactgt
    28561 atgctgttcc gctttattgg gcaggttttg aacaagcctc catgtggaaa caatttactc
    28621 ctgaaggaca gattaaatat gatttcttac aaaccgttac caaccttata ccgatgtaca
    28681 tctcccgtag tataggtggc actttatacc tggttggtgc attaatgatg gtttacaact
    28741 tgtataaaac tgtcaaaaaa ggaatattca taccaaatga agctgctgaa gcagcaccgc
    28801 tggtaaaaga ggtgagccat aagaaagaat actggcacag ggtaattgaa agaaaaccag
    28861 taaccatgct catactgagc cttatccttg tctctattgg tggtttggtg cagatgatac
    28921 ccactttcct ggtaaaatca aacataccta ctatcgccag cgtaaaacct tatactccac
    28981 ttgagctgca gggcagggat atttatgttc gtgaaggatg ttatacctgc cactcacaaa
    29041 tgatccgtcc tttccgtgat gaagtggcca ggtatggtga atactccaaa gccggtgaat
    29101 ttgtatacga ccatcctttt cagtggggaa gtaaaagaac gggtccggac ctggcaagag
    29161 aaggtgctcc taaattaaaa agaccagatt catggcatta caatcatatg tatgacccgc
    29221 ggtcaatgtc acccggatcg ctgatgccgc aatatgtatg gctgtttgac aataaattag
    29281 atactggttc tactcccgca aagatcaggg cgatgagaac attaggtgtt ccttatccta
    29341 agggttacga caaaatcgcc aacagggacc tgatgcacca ggcggattct attgcagcca
    29401 acctgaagaa agaaaaaatt gagagtcctt ccgatgtgga gatcattgca ttaatagctt
    29461 atctgcaacg cctgggccgg gatattacca atgaaccaaa accactggct gaaaacaaat
    29521 aaaatactga aaatgaaatt cattaattat ttagaaaaag taagcggtgt ggacattttt
    29581 ggcctggctt cacttataat tttcttttta ttttttttag taatgctcac ctgggtattt
    29641 aaaactaaaa agaaaagtta ccgggaaata agccgcattc cattagataa ctaatattaa
    29701 aactaacctg taatgctttt atctatgcgt aaaaaaacaa aatacatacc aggcatactg
    29761 gcctcgttgt taataatgac agcccagcct gtttgggcgg ccggtccgcc agaaccatct
    29821 attttaagca atccattggc gttgaccctt atgattctta tggctttgtt gctaatcatt
    29881 attggcatcc ttgctaatat attaataggt gccgctgatg ttaagcttaa aaaaagaaat
    29941 aagccgtcca gcaacaagcc tgtggcagca gcttcattga tacttgtact gttacttgca
    30001 agctcttcat tgtttgcaca ggatgccgcc ggtactgctg atgctgccaa cactgccgtc
    30061 aaaacgattg gggggatgtc tgcttccact ttttatataa tggttaccgt gttgtttctg
    30121 gagttattta ttataatagc gctgcttatt aatattaagt ttttactgaa aacagaaaaa
    30181 gaaaaactgg ctactgctgt agaaacagaa gaagttaaag ccaataaact tagctggtgg
    30241 gacaggttca acaagttgag tcccgttagt gaagaagctg atctggatct cggccatgat
    30301 tatgatggta tccgtgagct ggacaatcgt cttccaccgt ggtggctata cggattttat
    30361 ctttgtatca tatttgcagg tatttatctg tggcgtttcc atgtttcaca taccggtcct
    30421 tccagtaaac aggaatatga aagatcagta gcaagagcag atgctaaaat acaagagtac
    30481 ctgaaacaaa aaggagagtc tgtagatgaa aacaccgtga cgcttcttac cagtgccgat
    30541 gatatccagg caggaaaaac tatatttatg gatcctgcaa aatgccctgc ctgccacgga
    30601 acagatggtg gcggtaatgc cgttggtcct aatctcacag atgattactg gatctacgga
    30661 ggcagtatta aaacaatctt taagaccatt aaatatggcg ctaataatgg tatgaagagc
    30721 tggaaagatg aactatctgc taaacaaatg gcacaggtgg ccagctatat taaatcactc
    30781 aggggtacta aaccggctaa tccaaaagcg ccacagggtg aattgtataa ggaagaagca
    30841 gcccctgcag caccggctgc agattcaact gcagctgatg ccaagaaaat agcgatgaac
    30901 aattgaaatg acaatcacta atgaatgaag aaataaccaa cgagattcaa cccgaagaaa
    30961 ccccgaatga atcatttcgt aacaggatag ctaccgttga tgcaaaaggt aaaagaaaat
    31021 gggtgtttgc ccaaaaaccg caagggaaat tctataatat ccgtacctgg gtaagttggg
    31081 gattttttat acttttcttt gcgttaccgt ttataaaaat gaatgggaga cctttgttcc
    31141 tgttcaatgt tcctgatgcc aggtttatca tcttcggtaa aatattctgg ccccaggact
    31201 ttttcatttt tgggttaacg atggttacat tcatcatttt cattgtactt tttactgctg
    31261 cttttggccg gttattttgc ggatgggtat gtccccaaac gatcttcatg gaaatgttgt
    31321 tccgcaaagt ggaatatctt attgagggag atgccgcaaa acaaaaatta ttgaagaatt
    31381 caccctggac agctgaaaaa ataagaaaaa aagctacaaa acatatcgtc ttttacctgc
    31441 tggctttcat tatcgctaat tttttcctgg cttacattat tgggatggat gagctgaaaa
    31501 agatcattac tgaacctgta agcgaacaca ttataggttt catgtccatt cttttgtttt
    31561 caggtatttt ttatggggtg tatgcctttt tccgtgagca ggtttgtaca gttgtatgtc
    31621 cttatggccg tttacaaagt gttttgctgg atcgcaattc aatgcttgtt gcatatgatc
    31681 ataaacgagg agaaccaagg ggaaaatata aaaagaacca ggcagcagaa caaactgccg
    31741 gtgattgtat tgattgcttt caatgtgtga aagtttgtcc tacggggatt gatatccgaa
    31801 atggaacaca aatggaatgt gtgggctgta ctgcctgtat tgatgcctgt gacaaaatga
    31861 tggatgctgt tggaagacca aggggtctcg tccggtatgc ttctgagaat ggtattgaag
    31921 agggcaaaaa actgagctac actggcagaa tgaaattcta tactatcctt ttgatagtgc
    31981 taaccggcat ccttgctact ttgcttatta cccgcaaaga tatagacgga acgatagtaa
    32041 gaaccagggg gcaactttac caggagaggg ggactgacag cctttccaac ttgtttaaca
    32101 tcaaaatcat aaataaaaca atacacaata taccagtgac cctccggttg gaaggtgaag
    32161 ctgctaaagc tggaaaggtt gagatgatcg gatcaaaaga aataatttta aaacaggaag
    32221 accaggcaac aggatcattt tttattgttt taccgcggaa tttcatcacc aaaagaaaaa
    32281 tgaaattaga agtaggtctt tatgaagggg aaaagaaagt aactgccata aaaaccaatt
    32341 ttacggggcc tttcagcagg caataacaat attttattaa atttatttat atgaactggg
    32401 gtaataaatt attagtggca ttcattgttt tcggattagg gatgggttac ctggcctatc
    32461 gctcaatgaa taccaatttc gagttggtag aaaatgatta ctataagcag gaactccgtt
    32521 accagcaggt tattgatgga aaaaaagaag ctaataacct ggcattgccc gtaaatgtag
    32581 aacagacggc agatggaatt atactacagt tacctgaaga aatgaaaaac aaaactattt
    32641 ctggtaatat ctggttttac tgtgcgtatg atgcaaaaaa agataaaaaa ttcgctttaa
    32701 aaacaaataa tgccggaatg cagtcattcc agcctgagtc tatcaccccg ggaaattata
    32761 cagtaaagat taactggagt gatgaagaga aaaaatatta cgctgaaaag agtctaactg
    32821 tattataatg ttatgggctg ccatgatatc aggtttcaca ctcggagcag ccggcagcct
    32881 gcattgtgtt ggtatgtgcg gccccctgag cctcgcattg ccaacacatc atttaaccag
    32941 gacgcaaaaa tttttcttct tattgatcta ccaattcggc cggatcatta catatagtgg
    33001 tattgggttg gtctttggtc ttgccggcag aaggatatat atcagcggct atcagcaatg
    33061 gttctctatt gctatgggga tttttgtact ggcattagct gctatttatt ttattcaaaa
    33121 acacagcatt catttttctt tcctgaaccg tttctatcaa agcgttcagc aattaatcag
    33181 caatttgctg aaaagatcag gaggaccatt tagttttctg ctgcttggac tggcaaatgg
    33241 tttattacca tgcggactgg tatatgtagc agttgccgca acactttctt tcacagaaat
    33301 tgctgaaagc gtaagtttca tggcaatgtt tggcgccggt acattacccg caatgatgtt
    33361 agtgggctat gcaggacagc tggttaatgc tgaggcaagg caactgatgc gaaaagcagt
    33421 accggtattt attactttaa tgggaatcat acttattctc cgcggattga atttagggat
    33481 ccctttcatc agtccggaaa tgccacaatc tcccggtgaa gccgcagtct gtcatccaca
    33541 ataattattg ccggaaattt atttctctac tctttatctg aatagcccca ttcctggtct
    33601 tcctttaatt cagggtaatc agttctcccc aaaataataa cccggtaaac caaccataca
    33661 acagcaaagg gagagattaa aaaaataatt tctacaactg aaaataaatt tcctgtaagt
    33721 atgaggatac tatataacaa taacaggaca ctgacagcaa taaccggaat atattccgcc
    33781 ttgttcatat tcaaacaggt tgttacttat aacaatacaa gcgaaaaaag gttgattaaa
    33841 gtatgggggg ctccttatac tctggtaaac tgacagttac ttctgtaccc ttctcgagtt
    33901 cactcttcag gttaatatcc ccacccagta agtttacata ccgtttaact atcggaaggc
    33961 ccaaacccgt accctgtata ttacttacat tcttggccct gaaaaatgta ctgaaaagat
    34021 gaggttgatc ttctgccggg attcccaggc ccctatcttt cacggttatt atcaattccc
    34081 tgttacttac tacactctga atagtaactg tcttattttc ctgtgagaac ttaattgcat
    34141 ttgacaaaag attaagtaaa atatttttca tcattctctt atcagtggta aaattggggt
    34201 cacctgagca ttccgataaa atttcctgcc cgttttttaa atgagctttc atttcatcca
    34261 ccacatcttc aataaagtcc ctgacacaaa aagtggtaac cgtgatcccc acttttcctt
    34321 cttccagttt tccaagtgat aaaaaatctt ccagcaattc attcatgtga ttcacagatt
    34381 ccttaatcct cctgatatgt ttttctcttt tctcaaattc ctctgttgac ggatactttg
    34441 aaactaatgt agctgatgaa agaatagtgc tcaatggtgt tctgaattca tgagaagcca
    34501 ttgagacgaa ccggcttttg atctcattca gttgcctttc tttatcaagt gactcactaa
    34561 gttcctgctg cgattcttcc agtttttgca atgcctcctt taatataatg gtcctctcct
    34621 ctacttttcc ttccaggtcc gcattgagtt ttctgatgtc attactcacc ttctccagtt
    34681 ccattttctg acgaagcagg tttttctcaa tttccttacg ggctgtaata tcaacaataa
    34741 aaccaattac ataggattca ttcttctgca tgtaatgact aagacttact tcaacaggaa
    34801 attccgaatc gtcttttctc ctggcatata aatccctgcc ggttcccatt gaacggttgg
    34861 agggatgctt ataaaatcct tcccgtaatc cgtggtgctt accatgataa cgctgaggta
    34921 ttaatttctc aatctgttct cccatcattt cgtttgcagt atatccaaac attctttctg
    34981 ccgcgggatt tacaagtgta atcttacctg tacggtcagt aagaagaatt ccctccgttg
    35041 cattttcgaa aagtgacgcc atatgcgttt tttcatacgc aaaattttgt tgcattcgtt
    35101 tcaggtaaaa aaccacaatc acggcaaaaa ccacaacaaa caaagaatag acctgagaaa
    35161 gcagggcttt gattatctca ttctggtcat ttttaaaata aattacaagg gctgtcatca
    35221 caatcatact catgacacct gcaaccagtg ttggcctcca ccccggtata aaagctgtca
    35281 gcactatgat aataagcata ccacttactg ccagtttata gtctttaaaa aggaaggata
    35341 aggcaatagc aacggcaaaa acaaatattg cccaggcggt tattattcgg ctgttcagat
    35401 tatacattaa cccaatgaat taggagacaa aataagtata aaaacggagc gaaacagcac
    35461 ttgtcaatca atgccgctcc aaaagctctt tttccattgc aatggctttc ggaaaaagta
    35521 tatcattttc gagatacaaa tgctgaacaa gatcatcgtc cagctccttt aaaagcgaaa
    35581 aagcaagacg gtgactcgtg caggctgagt caggtgcagt ataatcattg gttagaaatc
    35641 tcaggcgatg caatgtcttt tccagtgttt catgttcatg atgcataata tcctcgaccg
    35701 gcttccttag cgtacggacc agcagactgg cataagattc tttactatca taagcatgag
    35761 ctatttgccg gatatagggg aaaattatct cctcctcttg cagtaaatgc gggatcattg
    35821 ttctgtctaa aaactcaatc tgggattcaa cttctggcag gtgagggtac tttttcagat
    35881 gctctgccac aaatttgccc aattgctcct ttacagaagg caaagattgc cgcaaatact
    35941 catgatgaat attaacgata taatctgtca ggaaatctac tttccaggta tcaaaaggca
    36001 gtgtgcccgg tatatttaaa acccggctgg ctcttttaag gtcatgtaat acttcttcta
    36061 cgtccagacc ctttgtttca cataccatct tgagcggcca ttttccacca caacaaaact
    36121 ccaccccata tttacggaat acggtggcag ttctgtaatg ctgctttaca atatctgcag
    36181 cacaagcgtt ttcatcaata acaatagaat gattaacctt catagcttct gtttcgccgg
    36241 caaaataata agatgcctga ccagaatacc tgatttaaat catcttaatg ttttaaaaaa
    36301 gttaatgctg tcttttgtac cccatacctg ccaaaaaatg taatgatcta tatcatttca
    36361 gcatatgaca aatatcactt taccggtttt ataccctgtg tacttttggt acagaggtga
    36421 aaaatacact taaaatgtgg ctcatgaaaa tgagctatcg ttggtttttg tttcaatgtc
    36481 aacccccggt tccccggggg tttctattta aaaaactatt tttgtaaaat gagagaagtt
    36541 gcttttatca ttggaggtat tattctatta ggagcttgtt ctaaaaacac ttcggctgac
    36601 aaagacacag aattaccagt tatcaccatt tctacacctt ccaataatca gctctttaat
    36661 aacggacaat ctataaacat caccggctct ataactgata atacatacat tgctgaagtg
    36721 catatacatg tcagtaatac aaatacaggg gcattgttaa tggatgtaca cctttatccc
    36781 ggcagcaatt caactacatt caatcagtct ttaacggcta tttcgggcat taactataaa
    36841 atccagatca ttgccaaaga ccgtgctgta aatgaagcga gaagttccgt tgaagcttcc
    36901 tgcaattagg cagttcctat ctttgccaaa tgcatataga tattctaact gttctcccgg
    36961 agttactgga aagtcctttt cagcatagta tgatgaaaag ggcccaggat aaaggattat
    37021 tgactataca ggttcatcaa cttcgtaaat gggccgtaaa cgaatacgga caaatagacg
    37081 attatcaata tggtggtggg gcaggtatgg taatgatgtg cgagccatta gcaaaagcaa
    37141 ttgaagaact atcactgcaa aaaaagtatg atgaagtgat atacctgaca cccgatggaa
    37201 atacatttaa tcagaaaact gcaaatgcct tatcactaaa gaaaaatctt ttactcatct
    37261 gcgggcatta taagggcatt gatgaaagaa tacgggaaca ttatgtaacc aaagaaatct
    37321 ctattggtga ttatgtacta agcggaggtg agctaccagc agcaatttta gtggatgcca
    37381 tcggaagatt gatacccgga gtattaaatg atgaaacctc tgccctcact gattctttcc
    37441 aggataattt attagcacca ccggtttaca caagacctgt ggattttcgt ggctggaaag
    37501 taccagatgc attaatgagt ggcaaccata agctcattga tgagtggcga catgaacaat
    37561 ccataaaacg aacccaggac agaagacctg atctgcttaa agaccaataa tgcttttttg
    37621 tattactttt aaaataattc tcatggccac ataaagtaaa ataaatgaaa aaaaaatatt
    37681 tttacccggt tgtagctata acgattttcg gtttgctatt ttcctgtact tcccctaaaa
    37741 aaacggtaag tgaccagaca tcaagccaac aatttcctaa agcattagct gaattcaggg
    37801 cagcatgggt tgctaccgtt gccaatatca actggccgag taaacccggt ttaagtactg
    37861 atgaacaaaa aaaggaagcc atcgaactgc ttgatttttt acagcaacat catttcaatg
    37921 cagttatatt acaggtaaga ccacaatgtg acgctttata caaaagcgat ctggaaccct
    37981 ggtcgtatta cctgaccggg aaacagggac aagctccctt tccctattat gatccacttg
    38041 aattctggac ggaagctgca catgaccgtg gaattgaaat gcatgtatgg ttaaatccat
    38101 accgggcaca tcataaagat ggaaaggaaa taacagaaca ttccattatt aaaaaacgtc
    38161 ctgaactggc cctttatcta aaagaaggtt actggtggct tgacccggcc caaaaaggaa
    38221 cgcaggatca ttcaactgct gtggtaatgg accttgttaa gcggtacgat attgatggtg
    38281 ttcattttga tgactacttt tatccatacc catcttataa tttgggtgcc gatttccccg
    38341 attctgtcag ttggaatgct taccagcaaa gtggtggtaa attatcgagg ggggattggc
    38401 ggcgtgaagc agtcaatgta ttcattgaga atttgtacaa aaaaataaaa gctgtaaagc
    38461 catttgtgaa atttggctta agtccatttg gtatctggcg gccgggttat cctgaatcag
    38521 tagcaggctt cgatcaatat gaacaattgt atgccgatgc caaactgtgg ttgaataaag
    38581 gatggatcga ttattttacc ccgcaattgt attggcccat caaccggcaa aatgccagtt
    38641 tcccggtact actgggctgg tgggccggag aaaataccat gaaaaggcat ttgtggcctg
    38701 gtatgagtgt gggcagggat accagtataa gatcagtcaa tgaaacactg aaccagatca
    38761 tgatcacaag aggaatggtt cctgaaagta aaggagctgt acactggagt atttcttctg
    38821 ttactaaaaa ccctaacctg gcaaaagcat tgttagaaag tacctataag aaccaggcta
    38881 ttgttcctgc aagtccctgg ctggataata aagctccgga tgcccccaaa gtttctacct
    38941 ccaaaacaga taaaataacc atcagctgga gtcatccgga tgaaagagat gtattcagat
    39001 gggtggtgta ttttaaatat ggcaataact ggaattatac tatcctcaac agaaatgaga
    39061 gatcttttac tttaaaccct gggacagaag taaacaaaaa aatagttgat ttgaaagagg
    39121 ttgccataac tgcagttgat cgaaccggaa atgaaagtgc cagaaatcaa accactatta
    39181 attaagaata tttcttatct gctataattt atcttgaact tgaagcaata ataaatgaat
    39241 ggccatatga gtaccaaccg aagaaacttt attcgccgca tagccatcgg cagcggtgtt
    39301 ttagccaccg gccttccttc ctttgccaaa gtaattggtg ccagtgatga acatatggaa
    39361 cgttcagttg attttgaaaa aagcgggcaa cgatttaata tgtgtggcta tgctgcccca
    39421 aaaatcgaaa cagttcgtat tggtataatc ggacttggta tgaggggttc tgatgcagta
    39481 gaaagactca gttatattga cggtgtggag atagttgctc tctgtgataa atatcccgac
    39541 cgggtggcgg catcgcaaaa gacactggaa aaaatggggc gacccaaagc catagcttac
    39601 agcggtgaag aaggatggaa ggctttatgt gagagtagtg atattgattt ggtctacaca
    39661 ccgactccct ggcacctgca tacacctgtt gcggtttatg ccatgatgaa tggtaaacat
    39721 gctgctgtag aagttcctgc cggaaaaacc atatatgaat tatgggaatt ggtggaaaca
    39781 tcagaaaaaa ctaagaagca ttgtatgatg ctggagaact gctgctatga cttcttcgaa
    39841 ctgctaactc tgaacatggc caggcaagga atgtttggcg aactggtgca tgcagaaggg
    39901 gcctatatcc atgaccttag caaagactgg aactttaata aaaaagctta tgccgatatg
    39961 tggcggttga aggagaatat cggtcacaat ggaaatcttt atcctactca tggccttgga
    40021 cccattgctc aatgcctgaa tatcaaccgc ggcgataaat ttgatcacct ggtaagtatg
    40081 agcaccaatg atttctcaat gaacaatata gcaaaggaac tggccgccaa agacgatttt
    40141 tttaaagaat ttgttgacag gccataccgg ggcaatatga atacaaccct gatccgtacc
    40201 aacaggggaa aaacagtaat ggtacagcat gatgtttcaa cactccggcc ctattcaaga
    40261 attcatctgg ttagtggcac caaaggtgcc gcacaaaaat ggcccggccc gcaacggatt
    40321 gcttttgggc atagctggct aaagcccgaa gaattgaaac aactggaaga aaaatatgct
    40381 ttgcccatcg taaaacatat tggcgctatc gccaaagaag taggcggaca tggaggcatg
    40441 gatttcatca tggattggag attgattgat tgtcttcgta atggactgcc attggatcag
    40501 gatgtatacg acgctgcatc ctggagttgc ctgatgcctt taagtgaaag gtctgtagcc
    40561 aaaaaatcga ggacggttga tatccctgat ttcacccgcg gtgcatggca aaccaataaa
    40621 ccggttgacc ttacattaaa tggtggagcc acaacagggg tcaggtcaat aaaacccgat
    40681 cttaaaatgc agtaatggaa atttacttga taaaaatcat gccgtgggta attaaacaaa
    40741 aaagccttat taaacagaat gctttaattt tacaccctcg caaatatctc tttacattgg
    40801 aaaccaagag gttactattt tcttaatacg gataaattcc cttttggaat tatttgaata
    40861 attaattttc gatgaaggca ctgacagtaa ttacagaccc cgtttatgta gaacctaaag
    40921 aatttaccgg atacgaaaaa ttctggttaa aatatatcaa tgacaaaaga gatctaccgt
    40981 ttattcattt gctaactgct attcatatac tggttattcc tgttgccatt ttattatata
    41041 caccggtatt gcaaagctgg tattggtggc tggtgtacat accttacttc tatgtttcgc
    41101 aaatgtattt taaaggccgg tttggattaa tgctgcattg tatcagccat cgcaaacttt
    41161 tcaaaaaagg ctatacctgg ctgtataatt atgttatctg gtttgtttgc cctttttttg
    41221 ggcatacccc ggaaacttat tttgctcatc atatgggcat gcaccatgta gaaaataata
    41281 tgctggacga tgccagcagt acattaccat accagcggga tagtattcgt gggttcctgt
    41341 cgtatgtggg tcgctttctg atccttggtt ttgaggacac ttttatgtat ttcttcaaac
    41401 gcaaaaggaa aaagttttac atgcggctta ctgcaggtga aatatcattt tacctttttt
    41461 gtgtgggaat gagttttgta aattttaaag ccacactgtt tattttcata attccatttg
    41521 tttttgcaag aatagtcatg atgctcggta actgggccca gcatgctttt gtcaaccttg
    41581 atgacctgga agacaacacg attaattgta ttaatacaaa gtataaccag atgtgctgga
    41641 atgatggcta tcatgctatc caccatgtac gtccggctat gcactataca gatattcccg
    41701 gtgaatttct taaaaccaaa gaccagctcg cagaaaaaaa agtattagta ttcgatggga
    41761 tacattacct gcatattttt ctatggttaa tgaccaagag atatgataag ctggctgaca
    41821 accttgtcaa tattaacaac atgtttaaca gcaaagaaga ggcgattgat ttaatgaaac
    41881 aacgcaccag gaaatataga atgaccgcat aattgaaagg gcttcctctg aagccctttc
    41941 tgatttttta ttccatctct ttactaagtc cgattacggg tagtgttttc tcatgtatca
    42001 gttggttcag tttcaacaaa tcctcctcta ctattttttt aagtttagtt aactctgcat
    42061 cggtttgaac agccaactcc gcataagctt cctttgcctg cctggatggt gctgtgtttc
    42121 ctgctgacgc tgcattgtaa acactggcta atttgtcatc caggcggatt ggataattca
    42181 acacatcctg gccactttta gctttggttt gatgtaatgc ctcttccact gccgtcattt
    42241 gtttattaat tgtatcaatc ttttgtttga tttcactcgg aatatcctta ccaatccggg
    42301 cattgaaatc agtaagttgc tgcctcacct cttttatatt cttcattgct ttcatcactt
    42361 cagaaaattt atcccgcacc gtcaacagaa aattaacctg ctcctcataa tcggcatttg
    42421 ttgttttata atttggatca cccaaaatgg caaaaccaac ttcaactgaa tctgaacctg
    42481 atttaaattt tgcgaaatat tcacccggtg ctgccttcgg tcctcttaca aatccattcc
    42541 agagtatcaa cccttccacc ctatctgccg gcgggtaatt catatcccag gtaaactggt
    42601 tcatgccctt attgatttca attttatttt cttttgaatt agtggcgaaa gttttgatta
    42661 actttttatt cttatctgaa atttctactg tgagcctggt tgagtcagta atatttttca
    42721 gaaaataatt catcaccact ccattgggcg gattgattcc cacatttcgc atcgttccgc
    42781 cgaaaccgcc aaacctgcct gaagcaggca tcctgtaggc ggggtttaca gtaaaaacat
    42841 gcaggttttt agttgtaatg ttttcatttc tctgctgtac aactgataaa tcatccagta
    42901 tgtaaaggct tctgccctgt gtggcaataa taaggtcgtt attttttata gccaggtctg
    42961 taaccggggt aacgggtaag ttcaattgaa acgatttcca gttagcacca tcgtcataag
    43021 atatatatat tccgtattca gttcctgcat ataagagacc ttgtctttta cgatcggctc
    43081 gtagtgccct ggtaaaatgt aaaggaggaa tactgtttgt aatctgtttc catgttttac
    43141 cataatcttc cgttttgaaa atgtatggct tgaaatcatc cagtttataa cgggtaccca
    43201 caaaataagc agctcctttt ttgaaaggat cagcttccac acaattccac atcatccacg
    43261 gcgggcaatc tttaggtgta acattttccc agttcttccc cccatttttg cttacatgaa
    43321 ttaaaccgtc atcactacct gaccataata aatctttttc atagggtgat tcagcggcag
    43381 taaaaacagt acaataatat tcaacggatg tattatcctt tgtcaccggg ccaccactgg
    43441 atgcctgttt ggttttatcg ttggtcgtaa ggtcagaaga aatctgttcc cagcttttac
    43501 cttcattttc tgttacaaat aaatgattac ctgccgcata caaccgtttc gggttatgtg
    43561 gtgaaaagaa gatggggtag ttccattgaa accggtattt cgctgcttct gcaccggcac
    43621 ccatattatc aatcggccaa acattgatca aacgactctc tccggtccga tgatcgagcc
    43681 gttgcagcat acccatataa tttccgccgt aggtgatatc aggattttct ggatctgcca
    43741 ccacataacc actttcactt cccgctgctg tttccatatc tgctgcagta atggcggcac
    43801 cgtacgtacg gctccggata cgaaacgcac tgttatcttg ctggccaccc ataattcggt
    43861 agggaaatgc attatctgta ctcagcctgt acacttgtgc ggtgggttgg tttaaataag
    43921 tactccagtt attaccggca tcaaaagtca cctgtccgcc tccatcatct gctacgatca
    43981 tcctgttacc atcttccggg tcgatccaca gatcatgatg atcgccatgt ggtgtgggga
    44041 tgctccggaa tgttttccct ccgtctgtac tgatcataaa atttacattc gggcaataca
    44101 ctttattctc attcttggga tccacataca ctttggtata gtaccaggcc cgctgacgga
    44161 tattattatc actgctggca agagtccagg tttcaccggc atctgtactt acatacaaac
    44221 cgcctttttc attttcgatc aacgcatata ttttgtctgt attcgaagga gccaccgcca
    44281 cacctactat cccccatgta tttttaggta gccctttcat tgtggaaata ttagtccagg
    44341 tttcaccacc atcaatactc ttccataaac cactgccttc cccgccactt tctaatgaat
    44401 gcggagtgcg gatcaaccgc caggtaccag cataaaaaac agaagggtta cccggttcca
    44461 taaccaggtc actgcaaccg gtttggttgt tgatatataa tacctttttc catgttttac
    44521 cgccatcagt tgttttgaaa actcctcttt cctgatttgg accaaacaaa tgtcccatca
    44581 ctgccaccca taccacatca gggtttttgg gatgtacgat gatccgaatg atatgacgac
    44641 catcgtttaa accgatattc ttccatgttc tgcctccatc ttcacttcgc cacatacccc
    44701 ccaagccttc acttacattt ccccgcatcg tattctcccc ttcaccgaca tataagatcg
    44761 tttcatcgga tggagctact gccactgcac cgatagatcc tccaaaatat ttatctgaaa
    44821 tatttgtcca gttacttcca ccatcagctg tcttccaaac accaccaccg gtgcccccga
    44881 aataaaaagt ggttttattc ttataactgc cggcaacagc tgcactacgt ccgccacgga
    44941 aagggccgat taaacggtat ttcaggtttt taaaaagaat ttcctcttta ttttctataa
    45001 caaccggagg aattggtttt gttttttgtg cagctaaaat gaatgcaaga ccaatcgtaa
    45061 ttggaagaag aatagctttt ctcataacag atatgtcaat tttatctttg aagttactga
    45121 tttaccttta accaaatcct aagcatgtct tcattttcta ttagtggtaa tttggttgac
    45181 gtacatcaac aaagaattta tcctgctgaa ctaactgttg ataaaggcaa gattatttct
    45241 attaaagaaa tttctcattc atcactcaac actccccact ttttgcttcc cggcttcatc
    45301 gacagtcatg tgcatattga aagttccatg ctggtgccat ctgagtttgc caggctggcc
    45361 gtagtgcatg gcactgttgc tactgtaagc gacccccatg aaattgccaa tgtttgtgga
    45421 ctggaaggtg tggagtttat gatagagaat ggaaaaacag ttccctttaa attcaatttt
    45481 ggcgccccga gctgtgtacc cgctaccact ttcgaaacgg caggtgcagt actggattcc
    45541 actgccgtag aaacactgct gcgaagaaaa gagataaaat atttgagtga gatgatgaat
    45601 tttcctggtg tattgtccag ggatgaagaa gtaatgaaga agattgctgc agcgcaccgg
    45661 ctgggtaaac ctgtagatgg acatgccccg ggtctcaggg gagaaaaggc taaacaatac
    45721 attgatgccg gtatctatac cgaccatgaa tgctttacag ctgaggaagc attggagaaa
    45781 ttacaatacg gaatgaaaat aattatccgg gaaggcagtg cagcaaaaaa ctttgaagca
    45841 ttaattgatt tactgcagga ctatccggac atgatgatgt tctgcagcga tgataaacat
    45901 ccggacagtt tggaatcagg acatatcaac cagctatgtg caagagctgc cgccaaaagc
    45961 atcgatattt ttaaaatact gaaagcagcc tgtgtcaatc ctgttcatca ttacaaattg
    46021 gatgttggat tattgagaga aggtgatgca gccgatttta ttgcagtgaa agatttgaag
    46081 aaatttgaag tggtgaaaac aatcatcaat ggtgaattgg ttgccgaaaa tggaatctcc
    46141 aacatcaaag cttcaaaatc agggattgta aataactttt cctgttcaaa aaaagaaact
    46201 gcccattttg agattccctt gaccacttac aactaccagc tccccgttat agaagcatta
    46261 gacgggcaat tgatcaccaa cagactttcg tttgaaccaa cagtaaaaga tgataaactt
    46321 gtaagtgata ccgatagaga tattctgaaa atagttgttg taaaccgtta taatgacgcc
    46381 ccggtagcta aagcttttat taaaaacttt ggactaaaac atggagcatt ggcctcttca
    46441 gtggcacatg acagtcataa tattgttgcc gtgggtgcag atgatgaaag tatctgtaat
    46501 gcagtgaacc tggtaatcga acatagagga ggagttagtt ttagtaatga cgatttggag
    46561 atgattgttc cattgcctgt ggcaggaatt atgagtaatg aagacggcta taaggttgca
    46621 gaacaatact cactgattga caaagctgtg aaagaagccg ggtccacgtt agggtcacca
    46681 tttatgacac tctcttttat ggccctgctc gtgataccgc atctgaagtt aagcgacaag
    46741 gggcttttcg atggcgatgc tttttgcttc gtttaaaaac actcggattt tctaaagtat
    46801 tgtattatat ttactaacgt cgcatttctg tcaatccttc acccctgcgt ttattgaaaa
    46861 caacattatt ctttctgact gggaaaccgc tattgttttc aataattcat aactattatg
    46921 gttaaagacg cattacgtat tgctgaatta gaaaaattgc tggccgaacg aaccgctgaa
    46981 ttaatacagc agggagaaga actggctgta ataaacagtg tacaggaagc cctggtcaag
    47041 gaaatggata tgcaggccat ttatgacctg gtgggaaata agatccagga actttttgat
    47101 gcacaggcag tgatcatttc cacctttgat catgagaacg gactggaaca attccagtat
    47161 acgatcgaag acggggaacg gttttacccc gagagccggc ctatcaataa gctgagaaaa
    47221 cacctgatag aatacaagca aaagatactg atcaatacgt cagcagaagc gcaggaatgg
    47281 tttggtaaag aagtggtacc cggcaccaaa ttaatgcaat ccggggtttt tgttcccctg
    47341 gttaccggcg atatcgttac cagttatgtc agtttacaaa atgttgaaaa ggaatatgcc
    47401 ttctccgatt ccgaagtacg gttgctggaa acactggcca acagcatgag caccgccctg
    47461 cagaatgccc gcctgctcaa agaaactgaa caacgaactg cagaacttgc agtgatcaat
    47521 agtgtacagg aaggattggc aaaggaatta gatatccagg ccatttatga tttagttggc
    47581 aatcgtatca gggagctttt tgatgcgcag gtggtgggca ttgccacttt cgatcatgaa
    47641 aaggcaactg aacatattca ttacctgatt gaaaaaggag aacggtatta tcctactcca
    47701 aaaccgatta attcattaag aagacatatc atcacaaacc gtgaaaaact ggtcatcaat
    47761 aaaaattttg caacagttgc tgatacgttt gggattaaac cggtgccagg cactcaacaa
    47821 ccaaaatccg ctgtatatgt tcccatgtta atcggggaca agatcactgg ttatgtaagt
    47881 ctccagaata ttgataagga aaatgctttc agcgaatctg atttgagatt attggaaacg
    47941 ttggccaata caatgggagt tgctttagaa aatgcaagac tctttgatga aaccacaaga
    48001 ctactgaaag aaaccgaaca gcaaaaagct gaactcggtg ttatcaacag tgtgcaggaa
    48061 gggctggcga aagaattgga tatgcaggcg atttatgacc tcgtcggtga ccgcatccgg
    48121 gcattattca atgcccaggc agtgatcatt tccacttttg atcatgatac ggcaacggaa
    48181 catttcaaat acctgatcga agatgggcaa aggttctacc tcgcttcccg gccttacgac
    48241 aaactaaggc agcacctgat cgtgaaccgg cagaaaattg ttatcaattc caactacgca
    48301 gaagcgtatg ccaaattcgg cttaaagaca ttgccgggta ctaatatgac aaaatcgggt
    48361 gtatttgtgc ctctaattat tggcgacaaa gtgaatagct atatcagttt acagaataat
    48421 gaaacggaaa atgctttcag cgaatctgat attcgtttgc tggaaacact ggctaacagt
    48481 atgagtgtag cattggagaa tgcccggctc tttgatgaaa ctacccgcct gttgaaagaa
    48541 accgaacagc gcactgccga actggctgtc atcaacagtg tacaagaggg actggcccgt
    48601 gaattggata tgaatgccat ttacgatctg gtcggcaaca aaatccggga tgtgtttgat
    48661 gcgcaggctg ttgtaatcgc cactttcgac catgaaaatg gaacggagaa attcaattac
    48721 ctgcttgaaa aaggggaacg ctactatgct gaaccaaggc gttatgataa actacgatac
    48781 cagttgatca tgacaaaaca cagaatactg atcaatgatg atgtagagaa agcatttaaa
    48841 gaattcggtt tgaaagtggt cgaggggacc gaaatgccca aatccttatt atatgtacct
    48901 ctggtggtag gaaataaagt aaccagttat gtaagcctgc aaaatgttga taagaacaat
    48961 gcatttagtg acgccgatgt tcgcctcctt gaaacactgg ctaacagtat gagtgtggca
    49021 ttggaaaatg caagactctt tgatgaaacc acccgactat tgaaagaaac tgaacagcgt
    49081 acagctgaat tggcagttat caacagcgta caggagggtt tggcgaaaga acttgatatg
    49141 cagggcattt acaatctcgt tggtgatcgg ttatgcgagt tnnnnnnnnn nnnnnnnnnn
    49201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49261 nnnnnnnnnn nnnnnnnnnn nttacagaat ctgacatgcg tttacttact actcttacca
    49321 acagtatgag tgtggccctg gaaaatgccc gcctttttga tgaaactact ctcctgctgg
    49381 ctgaatcaaa acaaagagca gctgaattaa gtactgtaaa tagtataagc aaagcattgg
    49441 cttcgcaatt aaatgctgat gacctgataa aaatggttgg tgaccagttg aaaaatttat
    49501 ttaaagctaa tattgtttat ctcgctttgt tgaattcaaa aacaaatatt gtcagtttcc
    49561 cttaccaata tggtgataat atggccccct taaaactagg ggaagggctg acctctagga
    49621 tcattatgac tggtgaaccg atccttatca acaaagacct gcaggaactg agggagcaga
    49681 tgggtattca gagtatcggc gttcccgcgg cttcctacct gggtgttcct ataccggttg
    49741 gtgatgaaaa tatcggagtg ctaagcgtac aaagtactga acatgaaaac cggttcaatg
    49801 aaaacgatct gcgtctctta acaactattg cctcttcggt gggtgttgct ttaaataatg
    49861 ctactctttt tgaagatgta aaactggcga agatggaagc agaagcagcg agtaatgccg
    49921 ccgaaaaagc caatgaagca aagagtgcat ttctttccac agtcagccat gaattaagaa
    49981 caccgttgac ctctgtattg ggtttcgcca agattatccg gaaaagatta gatgaaaaaa
    50041 tatttcccat cgttgacaga tcagataaaa aaacagacaa gacaatcagc cagatatctg
    50101 aaaacctcca ggtagttatc tcagaaggtg agcggcttac acatcttatt aatgatgtac
    50161 ttgacctcgc taaaattgaa gcaggcaaaa tggagtggag ccaggaaaat gtttccatgc
    50221 cggaagtggc agaaagggct attgcggcta ccacttcttt atttgatcag aaagcgttga
    50281 agctggttaa agaaattgat accaatcttc ctgaaattac cggcgataag gacaaactga
    50341 tacaggttat cattaatctt atttccaatt cagtaaagtt tacggataaa ggcactgtta
    50401 cttgccgggc ttaccaggaa aaagatgaat tgattgtcag catcagcgat accggtattg
    50461 gtattgcacc ggaggattat ggagcagtgt ttgaacaatt caaacaagtg ggcggagata
    50521 cattgaccga caaacctaaa ggaaccggcc ttggccttcc catctgtaaa gagattgtgg
    50581 aacatcacgg aggaagaata tgggtggaaa gtgaagtagg caaaggcagt actttttctt
    50641 ttgccttacc agtgaataaa acaacggcac atgccaggcc tttacacctt gatgacctgg
    50701 taaaacaatt aaaagaacag gtagcccatt cacaattaaa tataatcggc caggcatcca
    50761 atattttaat agtggacgat gatgactcta tccgctccct gctgcaacag gaactgggag
    50821 atgccggcta ttcgattgaa gaagccagca acggaaaaga agcacttgaa aaagtgagaa
    50881 ttaaccggcc cgacctgatt atcctcgata ttatgatgcc ggagatgaat ggatttgatg
    50941 tcgcggccat attgaaaaat gatccgaaca ccatggatat acctatcatc gtattatctg
    51001 ttgtgcagga caaagcaaga ggtttcagaa ttggagtaga caggtacctt accaaaccga
    51061 ttgatacagg tttgctgttt aatgagattg gcagtttgct ggaacagggc aaatcaaaaa
    51121 agaaagtgat gatagtggat gaagattctg gcgcagtaaa aacattgacc gatgtactca
    51181 ctaccaaagg atacgaggta acagaatcaa tagaaaaaga attggttgaa aaagcaattg
    51241 ccaatcagcc ggacatcatt atcattaact ctgcactgag tggcaaacat gatattgtac
    51301 aaacattgcg ttttgaaaag gggttagaga atgttttgtt tgtcgtgtac caataagaaa
    51361 agatttgaaa aaaaatataa tacaagactg aaattttgaa ccacaataga aaaattgtta
    51421 tacaaggttt tcacatagaa tttctttgtg tgaatttcta tattttacta tgatctatgt
    51481 tgttcaagct atataaactt agtattattg actgctaatg aaaaaaaatt gaattattaa
    51541 acaaaaagaa atggaacaaa aaatattaat cgtagatgac gaagcacata tccggatgct
    51601 tatcgggcag acattggaag agctggaaga tgaaggtgtg gaatttttaa cggcagagaa
    51661 cggagaaatt gcattggata ttattcagaa agagaaaccg caactggtat tcctggatgt
    51721 gatgatgccc aagatgaacg gaatggaagt atgccgccgg gtaaagcagg agctgaaaat
    51781 ggaaaatgtg tttattatac tgctaacagc caaagggcag gaactcgacc ggcaaaaagg
    51841 acaggaagtt ggcgctgatg tatatatgac caaacctttt gatcccgaag tgttacttat
    51901 caaagcaagg gaagtgatgg gcttggcgaa ttaatatttc tgtaaatagc ctttgatctt
    51961 tattttaaaa tagcgcagga aaaacatttt taataagtgg catgacaaga ataagtttaa
    52021 aaaatattat cggcaaaaag aatgaagccg ttgcaatgat acttgcctta attgagcaat
    52081 tgaatattat tgcctggata gaagatgatg ccggaaaaat attattgggt actgcccggg
    52141 aaacagcaga ttcaatattt cctgtaatag cagaagagga aatactgggc tttgtaaaag
    52201 gagaacaaaa agctgctatc atcgcccggc tgcttaccca tttaattaca aaagaatccg
    52261 aaaagaaaaa gcttggcagc gaagtgctgc atttatacca ggaagtaaac ctaatgtttg
    52321 acttctcaga taagctggcc aaaactattg atgctgccga tattgctaaa acaacgctgg
    52381 aagaagcaag ccgggtgatc cgttcagata acggggttat tattttatgg gatgaaaaaa
    52441 acaggcagtt acaggtattg gcttcccatg gaaatttatt ttttaaccag gaaaaaatca
    52501 atactgaact aaagatttta cttgatttaa tattaaatgg tcaatcagag atcatcagtg
    52561 acacttccgc attaagagcc acaggtattg tgcgaccaca tatcagctcc gttctttatt
    52621 cggctttgaa agtaaatcac cgggttatgg gtgctgtcat tctggccagt aatgaagaaa
    52681 tacaatatac tgcagccgac cttaaattgc tgaccaccct cgcactgcaa tcctcggctg
    52741 ccatcgaaag tgccttgcta tatgaaagaa atatcaagga tgcccttgaa agagaggagg
    52801 ccatgcgaaa attgtatgat gcaaccggta aatttgtccc ctatgaattc atccgttcac
    52861 tcggtcacca gttgatcacc gatgtaaagc tgggtgacca ggttgaaaaa attgtaaccg
    52921 tactattctc tgacatccgc gaatacacta ctatcgctga acaaatgacc cctgaagaaa
    52981 atttcaggtt tgtctgttcc tttaatgaac ggatgggtcc ttttatccgc caatacaatg
    53041 gattcatcaa ccaatatctc ggcgatgcta ttatggctat ttttccgggt aatgctaccg
    53101 acgcccttgc cgccgccgtg gcaatgcaaa aagaagtgga acaatttaat attgccagac
    53161 tgctgaacaa ccagactccc atccagatag gcgtgggtat gcatacagga ccacttatca
    53221 tgggcattac cggtgataaa gacaggatgg acgcttgtac aatttcagac acggtcaata
    53281 ctgcctcccg gctggaagga ctgaccaaac attataaaac tagcattata ctaagcgatg
    53341 caagtcttga acaaataaat aataaagaaa attttcacct gcgtaacctg ggtatggtgc
    53401 aattgaaagg caaacaggaa tccatcaaaa tacatgaatg tttcagcggc aatacaccaa
    53461 aggatattca aaaaaaggca gcgacccttt cccttttcaa cgaagggatc agccattacc
    53521 tggataaatc cttcaataaa gcccatgagg tttttacgca ggtaataaaa gaaaatcctg
    53581 aagacagaac agcagctttc ttttttacca atacaaaaca ggtaatggag gaaggatttt
    53641 ctaaaaaccg ggagggcatt gtggaaatgc aggaaaaatg aatggcagga aaaatggtaa
    53701 gtgggtgttc agccaataag cttcctattg aaaaacagcg tactgcatga cttgctgata
    53761 tctttttaat ccgtttctga ccgggaatta ggataattaa aaacgattat ttactttaat
    53821 ctggtaatta gaatttatgc tgttcgaatc atttcatttt attcatgatc atgtggtgca
    53881 taacctgctt accgggttat ccgaaaaact tacttatcac aacctgccac atacacttga
    53941 tgtgctaaaa caggttcagc gaatcggaaa agaagagggt atcatggagg aagaagatat
    54001 gtttttatta aaaacagctg cactttatca cgatacaggc tttttgtcag tttatgcagg
    54061 acacgaagca gccggttgtg aattagcccg gagggatttg cctggatttg ggttttctaa
    54121 aaaccagact gaacaggttt gcagcctgat tatggcgacc aaaattcccc aatcaccaaa
    54181 aaatatcctg gaagaaatta tctgtgatgc tgacctggat tacctgggtc gtgatgattt
    54241 tgaaccaatc agtgattccc ttcgaaggga gttcctggaa ttaggcattg taagttctga
    54301 tgaacactgg tattctctcc aaataaaatt tattgaaaat cacaattatt tcactaattc
    54361 atccaggcaa ctgcgcaacc ccgaaaaact taaacgtctg gaaaaattaa aatcagtata
    54421 gttttaaatg gtagacatac aaaaggtttg atgtattatt ttcctattca gatatgtatg
    54481 ctgaagtagt atcttaaagt attcaacagt aaaatacccc tcccgactgg ttcggattaa
    54541 tgattccaac aaccatttta ctttatcccg gctttatttc cgctatattt cattttttca
    54601 cattccctta ataagcaatc aacctgtttg ctgcatctaa taggtaaatg aggttaaaga
    54661 cccgcagggt tgattaagtg cggacagaaa gggtcggaac aaataaaccc tttgctatga
    54721 agcaatattt acatatttcc gccactttcg accagcccga attagtacag aacctggtcg
    54781 aacaattggc ttatcgtcgc tatgaactgg gattcagcaa tgagaaaatg ccccgtggtt
    54841 tcaaagcact ggaaacatca tctacagaaa catatgtcag cggtacgatg gctatagatt
    54901 ttggcactaa tgaagctcct gtcaatatgc cttttattac ttgcttcctg tttacctgta
    54961 tcaaaggtaa aactgaccct tatcaattga tctggagcag ctctctgtca taagacccat
    55021 aatttattat ataccttact tttcaccctt cctttcctgc cattcaccga gaaacagggg
    55081 catagtgccc attctctatt gaccattcac cattcgccat gtagttttgt ttaaaatttg
    55141 aaagcatgga cagctacaac gataaccgtg aacaacgcag ggaagaaagg aaggaacgcc
    55201 gtcaatttta tatgacaaac agcagccggc atagccatat ctggacaggg ctgttcatat
    55261 tgcttgttgg tgtagccgct ttgataaaag ccagtgtcac cgatttacct tcctggttat
    55321 ttggatggca aacatttctg atagccctcg gtatatttat aggcttcaaa catggtttca
    55381 ggggcggagc ctggtttatt ctcatcctag tgggtagtgc attcctgttg agggacattt
    55441 acccggattt agcaatccgt cgctatatct ggccgatgat attaatagtt attggcgcat
    55501 ttcttatcat ccgcccccgg cgaaaaagtt gggaacattg tgcatggaat gaaaaaaaaa
    55561 atacaggttc actaaacagc acatcgattt taggggaaga agaaacctgg agcaaagatg
    55621 attttgtgga ttccacttcc atcttcagtg gtgataaaaa aaatattctt tccaaagatt
    55681 ttaaaggagg agatattgtc aacatttttg gtggcactga attgaatcta agccaggctg
    55741 atattcaggg gaaagtagcg attgagataa ccaccatttt tggcggaacc aaactgatcg
    55801 ttccttccca ctgggaagtg aagtcagaag ccgtcaccat tttcggagga ctggaggata
    55861 aacggtcaat cactgcaatt ccggaaagtg gcggtaaagt tttactgttg aaaggaactg
    55921 ttatattcgg aggaatagaa ataaagagct tttaaatacg aagtcggttg acaattgact
    55981 gtcgggaaat ttcggataaa ccaaaaacca acctactatg aactggtatc ttgcaaaaat
    56041 ggtctttcgg atcatctgtg gtgaaggtaa tcataccgca cagtttgacg aacaactccg
    56101 cctcatttcg ggagaaagca aagaagaagc ttttcataaa gcacatatga tgggcatgaa
    56161 agaagaagag attttcttca ataaaaacat gcaattggtg cagtggcagt ttatcagcgt
    56221 aagtgagttg tatcagctta acgagctgat tgatggtgca gaactttact caaaaattga
    56281 ggaaagagaa aatgctgaca gctaccttca tatcgttcgt aaaaaagctg aacaaattag
    56341 atttggcaat tcgcttgaaa tgctgaaccc tgcatagcat gtctaactct cctttgtcca
    56401 tattacggtt taaagtaatt ttcattatct gctggctggt tatcataacc gaccaggcaa
    56461 ttgcattgaa tttttttggc ctgccctggc aagcagctat aatagatagt cttatttgta
    56521 atgcattatt attattagcc tgcttactta taatgcatac ccttcgttat tatttaccca
    56581 gaggacagca atacattaat attttttcta tttgtatctt tctttctatc ctgtggctga
    56641 ttctttccaa atggctgctc aaattgtcac ttggccatta cccgggttat actgatatgt
    56701 tgcatcaatc tctttctata cgcttcagta tcggctttct attactgggc tgtattacta
    56761 tgataagcat attatggtac aaccagcagg aacaaaaaga aaaagatacc cgtaaaacag
    56821 atgcagaaaa attagcgaag gaggcagaat tattcaagct ccgtcagcag ttacaaccac
    56881 atttcctgtt caatagtttg aattccatca atgcgctgat cgggagcagg cctgaagagg
    56941 caagaaaaat ggtgcagcaa ctttccgatt ttttaagggg tacgattaaa aaagaagaaa
    57001 cccaatgggt aaccctgcag gaagaattac aatatttaca attgtattta gatatagaaa
    57061 aggttcggtt tggcaaccgg ctggccacgg atataaatat agacgagaat acccaatcga
    57121 tgaaactgcc agccttatta ttacagccta ttgtggagaa tgccattaag tttggcctgt
    57181 atgatacaac cggtgaaacg gtcatacgat tgtatgcatc ccgggaagaa aataatctgg
    57241 tcataagggt aatgaacccg tttgatcccg aaacatcttc acccaaacaa ggaattggtt
    57301 ttggactaaa atcagtccag cggagactgt atctattatt tgccaggacg gatctattgt
    57361 ctacagaagc aaaagaaaat atctttataa cagtagttaa aattccggaa acaacataag
    57421 ttcttaaggt ttgctgcaaa gtaaaccggc aattgaaacc gggtaaaatt aaaaagcgaa
    57481 aaaatatagt atgggtaaaa taattattat tgatgatgaa ccgctggcca ggagcattgt
    57541 aagggagttc ctccaaaaac atccgcaact ggaagtaata caggaatgtg gtgatggctt
    57601 tgaaggtgta aaagccatcc agcagcacca gccggatttg atttttttag atattcaaat
    57661 gcccaagatc accggctttg aaatgctgga actgatcgaa caaccaccgg gtgttatttt
    57721 tactacggca tttgatgagt ttgctataaa agcatttgaa gctcatgcca tagattattt
    57781 attaaaacct ttcaaccagg accggtttga taaagccatt gggaaatgga ctgaacaaag
    57841 atccggcacc actgaaaaag caacacagga cttattggaa tctgcatcat tatccccctc
    57901 acagcgtcaa aggatcgtgg ttcggaatgg cagcaagatc aagatcattt cggtgcatga
    57961 tgtattttat ttagaagcgg cagatgacta tgtaaaaatt catacccagg aaggttattt
    58021 cctgaagaat aaaacgatga atcattttga acagtcatta gatatccatc agtttgtccg
    58081 ttctcaccgt tcatacattg taaatgtcca gcagattacg agaattgatc cgtatgaaaa
    58141 agataatcat gtagctattc ttcgttccgg cgctaaagta ccggtaagca gaagcggtta
    58201 tagtaaattg aaagaagtgc tggggctata gtttttaata acattcagaa ttcagcactt
    58261 ttaaagtttg atcataaaag tattttgccc tttcaaaagt ggaagcgata cagaccaaac
    58321 ctatttttcc atattgagat agggcgccaa tcaggtggaa catcacacct tcttcttttg
    58381 tgttatcgta gtgaaggtta ttgagcatgg caatttccat cagatcgcat ggagttaatc
    58441 ccttgaaccg gtcttcatgc aggttatccg taaacaggta acatttttca tcaccgttta
    58501 ctaaaacata cttacctgta tcaggattat agtcgccatt ggtcaaaaac tgtagcatga
    58561 tgtatgggtg agttgttcct cctttccgaa gatttatttc aatggcataa tgtttccatt
    58621 ggtcattttc tttgaccgat aaaaaatcga caccaaatct tcccaataca ccttttcgct
    58681 tcatcacttc tgatactttt ctacccaaag cagcaacttc aacactgtat tcttcatctg
    58741 ccggaaaggt cccccccaga aaaacctgtc catcgggccc tcccatcaat tggtcatgtg
    58801 tagaaataat atcaattttt cccaacgggt tgatgcggca ttgtacagaa ggggaagttt
    58861 tatgaggcgc atcaataaac gcttccacta tacctcccat actggtgaac ttgtcaataa
    58921 aaagttcata ggaaagatcc gtcgcaacaa tttccagcac ccggggtaac aattgccgga
    58981 tttcttcttc aacattttcg ttacttgtaa tagtatcaaa aggaaacaca gcatttccct
    59041 ccccgctaaa accgtcattc atcttaacaa cggctttttt taataatgga tcagcaatat
    59101 ataagtctgc taatgcttta attatctgtt cttcattctg aaggttctca taaccaggcg
    59161 gcatctccat accacattcc ctgaatatct cacggctgcc ggatttggtt ccccaaaaaa
    59221 gcaggtcagg gtcgcatcca taaactggta aaccaagagt agctgataat gtccgttcat
    59281 gttcagttac attaaaacat gccaggtgcg ccaggtgtcc atccggaata gcattcatga
    59341 ttctttttat caatcttggc ctggccagga ttttttcagt cagtgaaaca ggtgatgagt
    59401 cataacaact aaaaaaatgc attcgttgcc gggcatgctc ccctgttata cctggcaata
    59461 aatgaagata ataatctaaa atgagcggat caaccggggt gcttgtaaca tatacaatat
    59521 gtgtccttgg catccgcaat aagagaagta aacataacaa ccgctcttcg taatgaacaa
    59581 tcccatcaat tttggcgagt ataaatggat caagggtaag actgggaata acaacaacag
    59641 tcttggcagc catccgatca tgaaaaactg ctttgtattg tggcacaaaa ttttcctgga
    59701 gtttacaaaa aatctccttt tcagtcgggg cattttgact gaacagtttt atctgcttat
    59761 ccttagccaa tggcaataac cgttttagtt caggctaagc tactttaccc cactaaccga
    59821 tgcaatgata aaagagagaa ttattactga ttaatatcat gtatcaggct tcctgcccgg
    59881 tccataatgc tcccccaata ctatctcttg cagcacctgt aacggatgaa agtacattat
    59941 actcctgcct ccagcggagc actccaataa aggccataat aagggcttct ttgtaattga
    60001 taagcctttc gtcaggtact gccacttcaa ttttcaggtc tttaagatgg ctgttgagcc
    60061 gttctataag aaatgtattt aatgctcctc caccagtagc cagcaatagt gacccggttg
    60121 gcaccaggtc aggcgtaaaa agttgtacgg aagtaatggc tctttttatt tgtatagcaa
    60181 tatgttcgac aaaagttctt agcccatcta ctatactaca tcccgaatcc cgtatcatag
    60241 ggaatatcac atccgttcca aaatcattag ccagtgattt tggatatgat tggttgtaat
    60301 aagcaagatc attcagcttt tccagtaata caacgtttac acttccgctt gcagccattt
    60361 caccaccggc atcgtatact ttacccgcat tatttgccag catatttaaa acacggttag
    60421 ccggacaaac atcgaaagca acataaggat tagcattgac ggagatattg gcgataccgc
    60481 caatattcag aaaataagca tattccgtcc ataacagttt ctcacctatg ggcacaatcg
    60541 gagccccttg cccgccaaat gccacatcca aagccctgag atcagaaaca acgggcagtt
    60601 gtgtttcagc tgctatggca gcaccatcac ccaactgggc agtcattttt tgtgcaggga
    60661 gatgaaaagt tgtatgccca tgcgatgcaa tgacggcaac tttataatgc aattgatata
    60721 catctataaa cctgttcacc tgctgaccag tatagtgtcc ataatctgta tgcagtattt
    60781 gatactccct tgcagaaagg tttgtcgcat ttctcaggcg ttccacccat ttagcagaat
    60841 aggaataaca gtctgcctgt attatttcgt aagtccactt gccaccctgc tcctgaaact
    60901 caacaaatgc aatatcgagt ccgtcaagcg aactgccgct cattaatcca atagctctgt
    60961 aaaccatgaa accattttta gtttagattt gccaaaagta aatcttcaaa atgatagtca
    61021 atctcagcca gcaacattct ttgatcagta attgggtagg cgaactcaga gacgttacta
    61081 ttcagagcga ccggatgcgt tttcgccgca acctggaacg cattggggaa gttgctgcct
    61141 acgaaataag taaggtattg ccctttgagg aaaaagaaat tcaaaccccc cttggcacag
    61201 ccgtacataa agtattaaaa gaatatccgg tactggcaac cattctcagg gccgggcttc
    61261 cattgcacca gggattatta aattactttg atcaatcaga taatgcattt atctctgcat
    61321 atcgcaaaca tcataaggat ggcagttttg atataagcct cgattatgtt tcctgccctg
    61381 aattggagaa caggattgtt attatctctg atccgatgct ggccacgggt tcttccctcg
    61441 taaaaaccat tcacttttta aaagacgaag gacaaccccg tgaaattcat atcgtggcag
    61501 ctatagcctg cacagttggc atagaatatg taaaaagaga ggagccttct gttacaatct
    61561 ggtgtggtga tatagatgat gaacttaccg ccaagggcta tattgttccg gggcttggtg
    61621 atgcgggcga cctggcttat ggaccgaaaa tgcaaatgtg aaggagcaat tccagctttt
    61681 aaatctagag ttactggttt tattttcaat actttaagat tcaatttcgc ttctttaatt
    61741 tttttttgaa aaacacaccg gaagcagcat taattctatt cctcaggtct tcaaaatgtt
    61801 gtatttttcc gcttcgaccg ggtagaatta aagactagtt agggcaatca aactgaatac
    61861 taaaacgttt tcaatttctt atactctgat ttttgttaat caccggcctt acaactgaaa
    61921 aaaaatttat ttgttgagat gaaaaaaaat cttcttaccc tgttggtttg tgtggctatg
    61981 gcaagtgctt ctagagccca ggaccccaat ttttcccaat tttttgcctc gccacttacg
    62041 cttaatcctg cattgacagg gaagttcgat ggatctttca gggtggctgg aaactaccgt
    62101 aatcaatggc ctacgattaa taacgccttt gtaacgaaga ctgtatctgt tgattttggt
    62161 atcatgaaaa accggcttgc tgacattgac cagctaggtg ttggaattct tggcgtaaca
    62221 gacagagccg gtgatggtgt gctggttacc aattatgcag gagggtctgt tgcttttcat
    62281 aaaggactgg atgaagatgg ttttcaccag atcggtgcag gtttccaggg aacctatgtt
    62341 aataagcggt tggatgtaac caagattgat tttcaagatg aattgacccc gttgggtttt
    62401 acaggtgtaa ccagcgagat ttttttaaac aatcaactta acatcagtta ttttgatttg
    62461 aatgcaggtg tactttacaa cggcagtact aatggatata acaatttcta tatcggagct
    62521 tctatgtatc atatcaaccg cccgaaagaa agcttccagg gtggtgagta tctgttaaat
    62581 accagaacaa ctatacaggc cggaggaaaa ataccggttg gctcatataa ttatcttcat
    62641 attgcagcta atcacagtat gcaggctaaa gcaaataata ccgttgttgg aggagccttt
    62701 tcttacaatg taaataacaa tgaagatgac ccggtaaatg tgtacctggg tgcatggtac
    62761 cgtttcaatg atgcagctat cccctatata ggtcttgagt ttaaaggaat acagataggt
    62821 gcttcttatg atgccaatac ttccagtctt aaaccagctt caaatacaag gggcggaatg
    62881 gaattatccc ttatttacat taaaaagcct acagacccca atgctaaaaa attgaattgc
    62941 ccgagatttt aattgaactt ataggcaaaa gaaaagcgaa acatttaagt ttcgcttttc
    63001 ttttgcccat tcaaatcaaa tcttcattaa taatggtaat aaaaaccagg agaataatgt
    63061 aattaagatg atagagacaa tattcataat aaacccggct tttgtcattt gcctgagttt
    63121 tatatagccg ctggcaaaca caatcgcatt gggaggtgtg cccatcggca acatactggc
    63181 acagcttgcc gccaatgtca ttgggattcc cagtaacaat ggatctaatt tgagtgcaat
    63241 ggccaatgaa gtaactaccg gggcaaaaac aattacctgt gccacattac tcattacctc
    63301 actaaggaaa atggtgatta cagttatcgt aaaaaccaat ataaaaccat taccggaaaa
    63361 ttgagccagc catccaccca gctgttctat taatccagca ttttccaaag ctgtagcaag
    63421 ggaaatcccg cctccgaaaa gcaataaaat accccaggcc attttagctg tatcgttcca
    63481 ttcaagcaag gagcctgttc cttcctcatc tttactctct ctctttcccg agggacaaat
    63541 aaataacgcc agtgcacaaa gcatggcaat aatcgtatca tccagcttta taaatgattg
    63601 cgcattattg ataatgcttc ttgttatcca taataatgca gttgaaataa aaataataag
    63661 aacccttttt tccgcaaccg acaatgggcc caacaatagt atttcatttc ttattaatcc
    63721 ctttgtggct gtatcagatt taattcgact ggggaaaagc cattttacca tcacccaata
    63781 aaggcaaata agtaatataa tagccattgg catacatagc agcatccagt ttacaaaatc
    63841 aacggaagag tcgggaaatt ttttctctat atagccgata tatgccacat tcggcggcgt
    63901 accaataatg gtagcaatac ccccgatatt cgaagcataa gcaatagcca gcataatggt
    63961 aagagaaaaa ttagcaatat ttccgttgcc tgtattattc tctttcatta catgaataac
    64021 agacagtgca attggaaaca tcatcattgt tgtggcagta ttactcagcc acatacttaa
    64081 taatccggtt gcaagaatga aacccaatat aattctgtca ccactggtgc ccgtcagcct
    64141 tacaatattc agtgcaatac gacgatgcaa attccatttt tcaatggcaa gacccagcat
    64201 aaagccaccc ataaataaaa agataacagg attagcatac cctgaggcag ccgcttctaa
    64261 tttactgatc tttaaaagtg ggaaaagaac caatggcaat aaagcaacta caggcatcgg
    64321 taaagcctct gttacccacc aggtgatcat gagtgctgca actgccagta ctttgctggc
    64381 agtatgctcc acgccaaaag ggttactaaa ataaaggaat aaagcaataa cgatccctcc
    64441 cagcagagaa atagtttgaa cctgatattt cttaactttt atcaagatta gcgttttgga
    64501 ttttcaagat acaatcttgt cttgaatcag gaacatgtat atcatccttt ccgcaaaaaa
    64561 attgcttatt tcaatttccc gctaacatat aaattatctt tcaggtaaaa tcgatagggt
    64621 aataaagcat catctccggc ataatcaaca ccaatacggg cagtagacat caactgcttt
    64681 gcagaaacaa caaatccatc gtcagcaatg aataattctt tactttgtaa tgaatacccg
    64741 ctatgtgcag taacaattcc caaagccttt gctacgtttc cgggtccttt agtaagcgta
    64801 tagtcggctt ttttcttgcg agtccttacc agcatttccg caataccttc tattggctct
    64861 attgccctga tgagaatagc atgcggcaca tctttactgt gcgttaccac attaaaaaga
    64921 tgatgaattc cataacaaag ataaacatag gccaccccgc catgtgcata cattatctca
    64981 gtccggtttg ttcttctccc accaaatgca tgagaagctc tgtctattat tccattatat
    65041 gcctccagtt ctacaatccg cccggaagta actttaccat tccagtttgt gacaagtact
    65101 tttccaagaa gctcctttgc aatttgtaaa atattttccc tttggtaaaa ggatgccggt
    65161 aattttttca tattgcctgg tacaattatt acagattcaa tctgcagaaa tttactttca
    65221 ctttctattc gcaacttacc attccctttg tttatattta cttctcaaat taaggctttg
    65281 ctgaaagaaa tcgtcatagc ttttcaatcc tgggaagaag cccgtcgttt tattcaaaag
    65341 aaccggcttt ttaaatggat cattattccc ggtattattt ataccattct gttcatcgca
    65401 ggaatgtttt ttttctggca atcatctgac aatgttgttt catggataag taaccagctg
    65461 ggaatagaga actggctgca aaaagaaagg agtgaatggc tgagctttct ttttgtaatg
    65521 acaggcatga tgctccgtct tgtactggta ttgttttatt tctccctgtt caaatattta
    65581 atactaataa tcggttcccc tgttttcact tacctcagcg aaaaaacgga agcaatcatt
    65641 gatgagaaag aacatagttt caactggcca gaagttaaaa aagatgcgat ccggggtgtt
    65701 aaactggccc tgcgaaatgc aggctggcaa tctgtctatc ttattgcgct gatctttttg
    65761 tccctggttc ccgttattgg ctggattact cctgtcattg cactgcttat tgaatgctac
    65821 tattttggta tatctatgct tgactatagt tttgcgagaa acaattttag cctgtcgcaa
    65881 agtactgcat ttacaggtcg tcacaaaggt ctagcaatcg gcaacggcct tcttttctat
    65941 gccatgcata tccttattct cctggcacct gcttatgcta ttattgctgc aacattgagt
    66001 gtgcataaag ttaaaaacag ctgatacttc tgaaatgacc accgtttacc tgataccggc
    66061 aatactgcaa gatgaaggct ttgatgcaat tccaccctat ataagagatg caataaaaat
    66121 ttgccaggta ttttttgttg aaaatgaaag gacaacaaga cgttacttca aacagctctg
    66181 gaaagaaatg atcattgatg actacgaatg ggtgaatatg acagaaagtg ttattgcagc
    66241 cgatacattt agaaaaaaaa taaaagaagg aaaaaacata ggtatcgtca gtgaggcggg
    66301 ctgtcccgga gttgcggatc ccggccaggc tttggtagca atcgcacagg aaatgaatgt
    66361 taccgttaaa ccacttgttg gacccagttc catcttactt gccctgatgg caagtggaat
    66421 gaatggacag caatttaaat tctgcggcta tttaccgata gataacctcc tacgaataaa
    66481 agcgataaaa gaactggaag ctgaatcagc caggaaaaac tgcacccaga tctttattga
    66541 aaccccttac cgcaataacc aattagcaga tgccatcata aaaaattgca atccttctac
    66601 caaattatgc attgcagttg atcttacagg aaaaaatgaa tggataaaaa ctcaattgat
    66661 cagtgactgg gagaaaaaaa aaccggacat tcataaacgt ccggcgattt ttttactaca
    66721 tgcaaaatga tattattcaa aaggtgttgg cgtatttgaa ggtcgctgaa ttgaaataat
    66781 actgtccacc acatatttac tcattccccg ccatggtaat gcccctaaat actctttata
    66841 gtgcagctcc atgaatgccc cactgtttga cattaactgc aatgcaactt tttcatcaac
    66901 tactgaaaat tcaaactcgt tagactgtat actacccggg accttggatt tatatccggt
    66961 ttgtatcacc ctaccctcat aggttttaaa tatatacccc tttttaacaa agaagttcag
    67021 ttcaccagct ttactgccta caccaaatac aaagaagaat ttgaaatagg ttaatacagc
    67081 tacagctata aataacaaga tgagaattgt ccgggcaaat ttcctgaacc acgatttttt
    67141 ctttccaggt tcccgggcta acagttgttc ttgctgagtg agttgttgtt cgtccataaa
    67201 aaaggattta taacaaaaaa tttatgagtc taaaccaatt attattataa atttgcatcc
    67261 aagttacaac ttgtttgtct ttatggctaa cattctgaca catacaaagc agtttgcgtt
    67321 tcatgcgcct ataaccagcg gagtttattc tgttggcttt gatgtgttta ccttttcccg
    67381 cccccgacgt cgccctgtat tctgacagga cgaaccccct caccatggtc cctgtcagtg
    67441 aaagactccc cgaaaaaaaa ttaggattgg tggcccgttt tatatcaatg cagtattaaa
    67501 agtggacaat caaaatatta ttatcagagt agcaactgtt tccgacaaag tctattccaa
    67561 aactataaca gatgaaatgg cagcttcagc cgcagccagg ggcacgggta ttgcaaaacg
    67621 ttctccagaa tacattgagc aaaagattga tgaaggaaaa gccgttattg ctattaccgg
    67681 acagaatgaa tgggttggct tctgttacat cgaaacatgg agccatggtg agtatgtagc
    67741 caatagcggt ttgatagtag ctcctgcatt cagaaaaagt ggtgtagcca aatcaattaa
    67801 aaaaagaatc ttcgacctct cgagggaaaa atatccagag gcaaagcttt ttggattaac
    67861 tacaggctta gcggtaatga aaattaacag tgacctcggc tatgaacctg taacctattc
    67921 cgaacttacg caggatgaag cattctgggc aggctgtaaa agttgtgtaa attatgagat
    67981 actgatgagt aaggatagaa aaaactgtat gtgtacagcc atgttgtatg acccaaaaga
    68041 ccattacgag cctgaagaaa caaaacaatt ctttgaggaa aataagaaag gatttgaaag
    68101 actgttgaaa tttaagcaat ggaaactact taaaccgttt atgaaaaaag ataaagacgg
    68161 aaacggtggt gccagcaggc ccaaatcttt attacatcat ttatttcatt tataacaagc
    68221 actaaagaat caccatggca aaaaaagttg ttttagggtt tagtggtgga ttagatacat
    68281 cttattgtgt aaaatacctc agtgaggaca aaggttatga ggtacacagt atcatcgtca
    68341 acactggcgg cttcagtgat aaagaattga aacagattga agctcatgcc ttcaaactgg
    68401 gcgttaaaac acatacaacc attgatgccg taaaaggata ttatgaccgc attattaagt
    68461 acctcattta tggaaatgta cttaaaaata atacttaccc gctatcagtt tctgcagaaa
    68521 ggcttagcca ggcattgcat attgctgagc atgccaaaaa gctaaatgca gattccgtgg
    68581 cacatggaag taccggtgcc ggtaatgacc aggttcgctt tgatatgatt tttcatatca
    68641 tgatccccgg tgtagagatc atcaccccga tcagagattt aaagttgagc cgcgaagaag
    68701 agattgctta cctgaaaagc aaggctgtgg atatgaattt tgagaaagcg atgtactcta
    68761 tcaataaggg actgtgggga accagtgtgg gtggtaaaga aacactgtct tcaaagggta
    68821 tgctgcccga agaagcctgg ccaacgcagg ttaccaaatc aggagttgaa gaagtgagat
    68881 tagggtttga aaacggtgaa cttaaatcag taaatggaaa ttcatttact catcccgttg
    68941 aagctattca atatttacaa actattgccg gtccttatgg aattggccgt gatattcatg
    69001 ttggtgatac aatcatcggc atcaaaggca gggtaggttt tgaagcagct gcacctatgg
    69061 ttattctgaa agctcatcat gctttggaaa aacatgtatt gaccaaatgg caattacaat
    69121 ggaaagacca gttggctcaa ttttatggca actggttaca tgaagggcaa atacttgacc
    69181 cggtcatgag agacattgag gcattcctgg aaaatagcca gcaaaacgta actggtgaag
    69241 tatttgtaca acttatgcct taccgtttcc agttaattgg cattgaaagt aaatacgacc
    69301 tgatgagcag caagtttggt aaatacggcg aaatgaattc cggcttcacc ggtgatgatg
    69361 taagaggttt ttctaaaatt tttggtaacc agacatccat ttattaccag gttaaagaag
    69421 atgcagatgg aaaaaattaa agcaggtata atcgggggtg caggttacac cggaggtgaa
    69481 ttaatacgac tgttgttaaa tcaccccggt gtagagacct cttttattca tagccggagt
    69541 aatgccggta aatcggtttg cagtgtacac caggatctgg caggtgaaac agacttacaa
    69601 tttaccgccg aacttaatga tgatattgat gtactttttc tatgcctggg tcacggtgaa
    69661 tccagaaaat ttttaacaga gaaaaaaatc gctgacaaaa taaaagtaat tgatttggcg
    69721 aatgactttc gcctggcctc ctcctcattg attgataaca aacaattcgt ttacggtttg
    69781 ccggaactaa atagagaaaa gataaagtca gccaaccata tagcaaatcc tggttgtttt
    69841 gccacagcta tccaattagg attacttcca ttggcaaagg ccggtttact gaacgatata
    69901 tatacaacag gtatcaccgg ctcaacaggt gccggtcaga ctttgtcaac tacatctcac
    69961 tttagctgga gggcaaataa tattcaggct tataaaacac tatctcacca gcatttggga
    70021 gagattggag aatctttact tcacttgcag ccggctaatg atattgacct gagctttgtt
    70081 ccgtggagag gtgatttcac gagaggaatt tttatcagtt ctaccacaaa ttgtgatcta
    70141 agcattgaag aattacatgt actatactca actttttatg cagcccaccc ttttatttat
    70201 gtgagcaaag agccgatttt tttgaagcag g
//
LOCUS       DAORTI010000004        67035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_191_length_67035_cov_7.844162, whole genome shotgun sequence.
ACCESSION   DAORTI010000004 DAORTI010000000
VERSION     DAORTI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 67035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67035
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_67035_cov_7.844162"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLO70_01225"
     CDS             <1..498
                     /locus_tag="PLO70_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73103.1"
                     /translation="GKKDLLNEIKDLSDKQLSYVSPSTGLSVRKCMFQAAQTEKTMWE
                     ILNNAVKGPANPEKRLIISFTDNDLVERVKEGKLSPPVDIIFKQANAPWNNITAAVTD
                     FKDLRSDRIKYMRTSTEDLRNHVLQTSFGWIDCYQYILVITAETNRYIEQIKAIKSEK
                     KFPRK"
     gene            complement(499..2949)
                     /locus_tag="PLO70_01230"
     CDS             complement(499..2949)
                     /locus_tag="PLO70_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73104.1"
                     /translation="MKRSLLLLSILCIFLGADAQKPEELLGQLAKQSPIEKAYLHFDR
                     DNYLAGETAWFKAYLYSDYQPDTISSVLYVELLNESSVLISRKVLPVLLGCTNGQIEL
                     PDSLITGTYFIRTYSPTMLNNGIDFIYKKDIFIFGKKDKQAVANKQDEKSIQLEFFPE
                     GGNLVEGLMNTIAFKATDEKGIPVAVRGTISNNKNEKITSFSSYHDGMGMFELIPQAG
                     LNYFASFEADVTAKKYSLPDITANGVTLTMIPHPQGIFFEIRQRVTDPSMRAAYMIGQ
                     MQHHVVFRQELASGKNEIQGVVNTEKLNSGILQITVFNKEGIPLAERLCFVNNKEYIQ
                     KADLRTDTLSFLEKGRNHFKIVMKDTVQGNLSVSITDADYSLATYRRETIFSNFLLTS
                     DLRGDINNPAWYFSSDEDSVKTALDLVMMTNGWRRFRWNELAQKVKNPLNYSDPSYIT
                     ITGKVTMQGSNRPFAEKPMVAIISAEGMSRNFQMINTDKNGFFKLDSLVFFGNGRIFI
                     LDIRGKRSQFIDVKLSGDTAAKIFSLPVGTVHFPAKENVSPAEQNKLTFDFDAIQKEK
                     GILLEDVIINVKKKSATDELNERYTRGLFSGEAVRTIDMVNTSDIVVQNNIFDYLQSR
                     VPGLSVVEPDYSTSSLPSLENPFDDPTGYRLYYRQGPSASSLGNIPMVIYLDEIETSS
                     AVVATIPANQIAMIKVFSFFAAAPGGGSGGALAIYTKKGQDIKNSSRGDLISYKGFSV
                     VKEFYAPNYKAEPELLSKGDSRITLDWRPNIFINNVNPVIPVSFYNSDRTKKFRVVVE
                     GMTTSGKMISLEKIIEAR"
     gene            3106..3516
                     /locus_tag="PLO70_01235"
     CDS             3106..3516
                     /locus_tag="PLO70_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73105.1"
                     /translation="MKKLTFILGLAILLSGCKKTVEQIQQDLVIQAMTDGQWAITSFT
                     VNGTNITIDFTNYRFKYYSNRTVDAINSGTVEKTGTWDGNATTMTTSANFTAASYPLT
                     LINGSWLITRNSWTYVEATQTSGADIKTMRLEKQ"
     gene            3718..8073
                     /locus_tag="PLO70_01240"
     CDS             3718..8073
                     /locus_tag="PLO70_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73106.1"
                     /translation="MKPLYHNIPGRFFIFWFFIFGAIAARTQPVANFTASQQVGCSPI
                     IVNFQDQSAGNPTSWLWNFGNGNTSTLQNPTASYFTPGTYTVTLTVSNAGGTNTIVRT
                     DYITVYEPPSVNFSANITSGCFPLRVQFTDLSTAGLNNTNVTWDWDLGNGTTSSLQNP
                     SVTYTNAGTYTVTLRVTNDKGCTRTISRPNFITVTPGVRSSFTHTQPTVCSAPANISF
                     TNTSTGPPTLTYLWDFGDGNTSTLLNPVHTYTVNGTYTATLVTFSSAGCQDTARSNPI
                     IIGGYNTSFSYPASVCINEIVNFTNTSTPIPVNANWTFGDGGTASGINTTYSYPVPGV
                     YNVRLYNTYGNCIDSVDQNITVNPRPVADFMAPVTSRCEPPLTVNFQDLSTGGAVAWQ
                     WDFGDGNTSTQQNPLHTYTNYGNFTVTLIATNVFGCTDTITRTDYIRIRRAAISIPAL
                     PARGCIPFTISPVPVISALDAVTSFEWDFGDGGTSFLPNPTHTYTVQGTYTVRLIITT
                     STGCRDTLNIPFSVQVGSKPVANFSATPNPVCARKPVQFTDLSVPADEWYWDFGDGST
                     STLQNPVHNYTDTGYFTIRLIALNNGCADTITIPNYIYVLPPIARFTYTPDCGNRLRF
                     SFTDQSVGPLTWEWDFGDGSPVVNLQNPVHLYSSLGTYPVQLIVTNGGCADTTIQTIN
                     AVDERADFTADQTIICKTGTVVFTPTGFNLLNITSYTWDFGNGVTSTVSNPVISNAYP
                     NSGTYTVTLTITDINGCTNTIVKNNYIRVNGPTANFSGTNVAGCTGLTATFNDLSTTD
                     GQNNITNWQWHFGDGNIQNFSAPPFQYTYNTTGTYSVKLVITDASGCKDSITLANLVT
                     ATDPIPDFVVDTLACPGAILTFTNTTSPAGLNSFWDFGDGNTSATLSPTHSYTAAGLY
                     TVKLRVQDVYGCADSITRTNYIRVGSPVPDFNISDSISSCTPLEVQFTNTSTFYNSVL
                     WDFGPGQGTSSLINPVHYYSIPGIYPVKLIVTSPGGCQDSIIKTIRVYDTAGSRLNYI
                     PVQGCKPLVIDLNVLTSGPMESYFWDFGDGYTQITTVPNASHVYSTFGNFLPKVILED
                     PSGCIIPLQGFDTAFIIGAVAKFGVDTTFFCDFGTVNFLDSTTFNDPIDVYNWDFGDG
                     NISTLQNPVHQYSSPGLYTVQLMVQTQQGCRDTVTKPALIKVIQRPLIDISGDSVICI
                     NSSILHSGIFLQPDTSVVSWQWTFPNGNTSTLQNPPQQTYSVAGSFVATAIATNSSGC
                     KDTTRQTIIVNPIPVVTMPGQITIQSGFPVTIPATYSPNTVSWIWSPSAGLSCANCPT
                     PDAVPKFNTTYQVYFTDDNNCSNTGKIQIIVICKDGNLFIPNTFSPNGDGSNDVFYPR
                     GKGLERVKMLRIFNRWGEVVFEKRDFPVNDASAGWNGSYKGQKPKADVYVYQAEVFCD
                     NGDIIRLNGNIALIL"
     gene            8076..9089
                     /locus_tag="PLO70_01245"
     CDS             8076..9089
                     /locus_tag="PLO70_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQZ73107.1"
                     /translation="MKLKLTIKYISLLFTAFFSQLAVSSQDIHFSQFFEAPLWRNPSL
                     AGIFTGDVRVQAVYRDQWNSVTNAYKTGSVNAEYKMPVGKVNDFVTVGMQVLYDRAGT
                     IGWTSTHILPALNYHKSLSTENNRYLSLGFMGGLVQRRFDPSKMTTNSQYDNGGLGEN
                     LANTKYSYLDGSVGMSYNSNLNYNPNNNFYVGVAYHHFNRPNTSFYRNANIELYPKWV
                     FSGGLRFGVSDYAYMTLQADQSMQGSFSETMAGVMYGLKLGEDPDNPAYTIHAGGFLR
                     WNDALIPVIKLDYNPFSVALSYDVNISKLKPSSYGRGGFELSVSYIGFLKRNEGYLLC
                     PKF"
     gene            complement(9155..9856)
                     /gene="aqpZ"
                     /locus_tag="PLO70_01250"
     CDS             complement(9155..9856)
                     /gene="aqpZ"
                     /locus_tag="PLO70_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018764411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQZ73108.1"
                     /translation="MKKLLAELIGTFSLVLFGCGAAVVSGVSTSGPSGIGLLGISFAF
                     GLAVVAMAYAIGGISGCHINPAITISMLVAGKIKAGEAIQYILGQLTGATLAAGVLYV
                     IQKGSPGFSMGEWALGSNGWGEGYLGGYNMTSAFITEAVLTFLFLFVIFGTTSKWGNG
                     TMAGLSIGLTLVLIHLVAIPVTGTSVNPARSFGPAVFAQGKALTQLWLFIVAPIVGGV
                     AAALVWKGLFETKGE"
     gene            9973..10440
                     /locus_tag="PLO70_01255"
     CDS             9973..10440
                     /locus_tag="PLO70_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQZ73109.1"
                     /translation="MSIQIGQAAPDFTLFDSDKNPVKLSEQKGNNVLLLFFPQAFTSV
                     CTKELCAVRDNIALYNNANAKVFGISVDSVFTLAKFKEEQQFNFPLLSDFNKEVSGNF
                     ETIYKDWILNMKGVSKRSAFVIDKKGNIQYAEVLESAGDVPDFEKINAVLNNL"
     gene            10577..10921
                     /locus_tag="PLO70_01260"
     CDS             10577..10921
                     /locus_tag="PLO70_01260"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ73110.1"
                     /translation="MRRILPILSFILLIALNSAGQPSRTNPSGDGDRIVKLYPNPATS
                     YITFDLQNNFRKGLTVQIYNGVLGKKMFETQNIPDKFTINLNDFIRGIYIYHLVDMTG
                     KIIESGKFQVSR"
     gene            complement(10940..12112)
                     /locus_tag="PLO70_01265"
     CDS             complement(10940..12112)
                     /locus_tag="PLO70_01265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQZ73111.1"
                     /translation="MNKVYLKRKISLRVANGHPWIFNNEVEKIEGDVQGGDVVEVLDH
                     DKKFIGKGYINPKSQILVRLLTRTRTDEINEQYFIEQISKCWEYRKRIGYTENCRLVF
                     GEADSLPQLIIDKFNDYFVIQTLALGIDVWKPAIVNALNTIFQPHGIYERNDVPVREL
                     EGLPQQKGFLSAPFDTKIIINENGLKFHVDVENGQKTGYFLDQQDNRRAIQHIVKGAD
                     VLGAFTYTGTFEIHAAQYGAKSVLGLDISENAVAQANRNAELNGVQDRCRFETANAFD
                     VLKQWGKDGRQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKLVKPGGFLVTSSC
                     TNLVNPELFLQTIEMAAKDVRRKIRQVTFQTQSSDHPIIWGMDNTQYLKFLIVQVQ"
     gene            12301..12873
                     /locus_tag="PLO70_01270"
     CDS             12301..12873
                     /locus_tag="PLO70_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQZ73112.1"
                     /translation="MAEKDMKQQESTLPDNNSGLDINTDENLSGTTHLNEPVSEESEL
                     EKLRTELEESKDKYLRLVAEFDNFKRRNAKERIELIQTAGKEVITDLLDVLDDCDRAQ
                     KQLETNNNSEAIKEGVLLVFNKLRSTLQSRGAKPMETLNKEFNPDLHEAITEIPAPAA
                     DMKGKVVDEVVKGYYLNDKIIRHAKVVVGK"
     gene            12910..14079
                     /gene="dnaJ"
                     /locus_tag="PLO70_01275"
     CDS             12910..14079
                     /gene="dnaJ"
                     /locus_tag="PLO70_01275"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQZ73113.1"
                     /translation="MSKRDYYEILGVSKTASADEIKKAYRKVAMQYHPDRNPGDKAAE
                     EKFKEAAEAYEVLSDTDKKAQYDRYGHAGVTGNGRGGFGGGGAGMNMDDIFSQFGDIF
                     GEDLFGNFFGGAQRGGRGGQRSRGVRGSNLRVKLKLTYEEIAKGVTKNIKVKKHVICS
                     ACSGSGAKDKGSVQTCGTCGGSGQVRRVQNTFLGQMQTVTTCPTCNGEGTTVTAKCTS
                     CKGEGRVYGEETVSIDIPAGVQEGMQMNVNGKGNAGERGGMPGDLIILIEEEPHKELH
                     REGLNVAYELHLSFTDAVFGTQVEVPTIDGRAKIKIPPGTQSGKIFRLKGKGFPAVNS
                     YEKGDQLIQVSVWTPQHLSTEEKAALEKLSHSPNFKPHPDKNDKNFFDKIKEMFS"
     gene            complement(14081..15889)
                     /locus_tag="PLO70_01280"
     CDS             complement(14081..15889)
                     /locus_tag="PLO70_01280"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HQZ73114.1"
                     /translation="MYFIDQCIQLKAACHILVFLIFSFSLYAQQYYVRGEVKDESGNI
                     LQNVTIRQYKTGYIFRSGSAGTFGIVANHQVDTFSFSLVGYRTETLVVDAEHYVNVKL
                     RLLPASVSNSRRDKLSSFTKDLGKEEQKKWFTGDETYASLLENHFVNAKRFPNTGMSL
                     NVDRASYSNIRRFITLNSMVPPDAVRIEEMLNYFNLNYHEPAANELFHLKTTLSSCPW
                     NQDNQLFYIDLSSRKLNLDTLPPSHLVFLIDVSGSMDMPNRLPLLQSAFRLLVNNLRD
                     KDSVAIVVYGGVTGVMLNTTSGGEKEIILKAIDELTPGGSTPGESGIKLAYSVARNHF
                     IKGGNNRVILATDGDFNVGLKTETELDEMITAQRESGVYLTCLGVGMGNYKDSKIHLL
                     AEKGNGNFAYLDNFKEAEKVLLKEFTQTLYSVADDVYMNVEFNPEYVKDYRLVGFDNK
                     VGALRDSLSIIEGGEIGSGHSMIAMFELVPTEINKGAIKDNFTTGKLADIKLQYRHPN
                     DTTHHYFNYTSRFDFIPFAEIDKCYHFSASVAMFGSLLRSSPFVKNIGWNEVVTLAQS
                     SSGNNDLLQKEFITLVQQAKTLYSKVKKKKSGGANW"
     gene            complement(15915..16448)
                     /gene="hpt"
                     /locus_tag="PLO70_01285"
     CDS             complement(15915..16448)
                     /gene="hpt"
                     /locus_tag="PLO70_01285"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQZ73115.1"
                     /translation="MAIIRVHDKSFETYLPAETIQQRIKELAGIINKDYAGKKPLFIA
                     ILNGSFMFAADLFKHLTIEAEICFIKLASYKGMKSSGNVVTSIGLEDDLFGKEVIIVE
                     DIVDTGKTLHNFLPKLLHQQPKSMKIATLLYKSEAIEYPLVLDYVGFDIPNKFVVGYG
                     LDYDGLGRNLKEIYQLV"
     gene            complement(16466..17527)
                     /locus_tag="PLO70_01290"
     CDS             complement(16466..17527)
                     /locus_tag="PLO70_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HQZ73116.1"
                     /translation="MQNVRIILIFFLSLTAFTAAAQNSEVVKNYINIYKSLAMAEMQR
                     TGVPASIKLAQGIHETMAGTSKLVLKSNNHFGIKCKSNWTGESVSHDDDARGECFRKY
                     SSPEDSYRDHSNFLKNSQRYASLFILDPTDYTGWANGLKNAGYATNPKYPQVLIKLIE
                     DYNLQEYTLIALGQQQAKPDEMASIPEMNKEITIPSTPSAAITVAGENQSVKKSEYPK
                     GEFRINDTRVVYIQQGTSFLSIAQQYNVPLARLFEFNEISQSEASEKDQLIYLQRKRK
                     TGSEEFYIVQPEENLHEIAQKQGIRLESLLELNWLKNNELPAPGEQLSLKKKSSAMPK
                     LAIKENFSLNSGRRIQATN"
     gene            complement(17542..18180)
                     /locus_tag="PLO70_01295"
     CDS             complement(17542..18180)
                     /locus_tag="PLO70_01295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HQZ73117.1"
                     /translation="MELINPLVEKYAEKYSSAEDILLKEVNEFTLQNHSESIMLSGHL
                     QGKVLEMISSMIRPGRILEIGTFTGYSALCLAKGLTEDGLLHTIELREKDAARARDFF
                     DRSAHAGQIISHTGSALDIIPRLEETWDLVFIDADKPAYIEYFNLVFPRLRKNGFILA
                     DNIFFHGQVLEENVKGKSAKGIKAFNEFIKGRTDIEKVILTIRDGLFLIRKL"
     gene            complement(18208..19737)
                     /locus_tag="PLO70_01300"
     CDS             complement(18208..19737)
                     /locus_tag="PLO70_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQZ73118.1"
                     /translation="MSPIVLFSFVIGYFVLLLGVAWYTSRNSDNESFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGGVGAGNFYYYQVVLGYLLGYMVIAFVLIPLYYRMNLTS
                     IYTYLERRLGINAHKAGAFFFILSRTVGATARLYLVISVLQIFIFNRLGIPFEATALV
                     ILLLILLYTFEGGVKTIIYTDTLQTTGMLVGLVACIIVIIKALGTDFGGAMSMMADKG
                     YTQIFNWDVKAGSYVWKHIVGGMFIAIAMTGLDQEMMQKNISVKTLKDSQKNIMTFTA
                     VLMVVNFLFLVLGGVLYLYAGTKGINVPPDDLFPTIALSDTFSGVIGIIFIIALISAL
                     FPSVDGAITSLTSCFCIDILGINKMAGTEKEKKRTRLKVHFTFALVFFIMVLVFKWIN
                     DKLIIDFILKFAGITYGPLLGLFAFGILTKRELNNKLIWTVCIVAPLLALSVDMLSNP
                     AWYDAKLHLSLGLQDLSNKIFNGYKIGNELILINGIFTFLGLWFISKKPSLSKLKLQA
                     V"
     gene            19839..20375
                     /locus_tag="PLO70_01305"
     CDS             19839..20375
                     /locus_tag="PLO70_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQZ73119.1"
                     /translation="MAIQSLAVFCGSKNGTNPVFGQHASQLGKLMAENNIRLIYGGGS
                     AGIMGNIADNVMQHGGKVTGIIPKLLLEWEVQHRGITELIICDDMHERKKKLYSLCDA
                     ALILPGGFGTLDELFEIVTWNQLTIHDKEIYILNSGGFYNHLIKHIEIMKAEQFLYEE
                     AIKRITIIDDPSELTRYL"
     gene            complement(20382..20990)
                     /locus_tag="PLO70_01310"
     CDS             complement(20382..20990)
                     /locus_tag="PLO70_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73120.1"
                     /translation="MKRTFLLLAFIASAAVSFAQNEEEETKPGFKENLFTGGSISLSF
                     FNNTFLIGGSPVFGYSLSNWADAGIVVNYNYTSYRDYNYGFNDKLRQTVYGGGAFVKL
                     YPLRFLFAQAQFEHNFIRQKFIPNSGATQTYSTSGNSMLVGGGYTTGRQGKGGQPFYY
                     LAVLFDVSGNDNSPYTDAYGRTIPIIRGGIQVPLFQGDQWRR"
     gene            complement(21013..21507)
                     /locus_tag="PLO70_01315"
     CDS             complement(21013..21507)
                     /locus_tag="PLO70_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73121.1"
                     /translation="MRKQIEFTGSPKENLRAMRILTAALSTGVIFFALIAVVINQLNG
                     PVQGQAGSNYETLFLWIVVVVASICIIVAKYLYKRKVSEIKNTGKSLSDKLNQYRSAL
                     ILYQALCEAAALLSVVVFFLTGIFTLLIITAILLTLMLSKVPITKKVVNELALDWKEQ
                     QEFE"
     gene            complement(21622..22656)
                     /locus_tag="PLO70_01320"
     CDS             complement(21622..22656)
                     /locus_tag="PLO70_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQZ73122.1"
                     /translation="MNNIRNIILLAVCGLGLNAHSQDLHFSQFMNSPLLTNPANTGFI
                     PDGDYRLGVNYRNQWASITAFPYKTMSAFGDVQLMQNSENTGWLGLGGVILRDVAGSS
                     VLTSTKVYGSVAYHQMINAGSLVSLGFNAGWANKNINTSNLSFPSQWNGQFFDAHQTT
                     AAPKLDYNNINYIDMQVGMNYAYFPNTSTYLNAGFSAMHINQPRESFFNEQPGIDNRI
                     PVRYTGFINGSFKLNDMVIVNPNAYFSLQAKSYEIVGGLNAHYNLSGDGDKILMAGAY
                     YRYKDAVIPMVGLGIKDITFTFTYDATTSSLKNFNNTRGAFEFSLIKQGSRDAYRGNK
                     RESMCPSFKY"
     gene            complement(22693..26367)
                     /locus_tag="PLO70_01325"
     CDS             complement(22693..26367)
                     /locus_tag="PLO70_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73123.1"
                     /translation="MTRQLLTEVFNPFPGKIGYFNENNLTLKYLFILAVFLFPALTVP
                     AQGNIEFIENKGQWDSRVQYKGDVSNGVFFIRNGGFTVVQHNPADVARMSQFKHGLNG
                     SGKPLAANESLVIRSHAWNVDFIGASKNLKLIPEKPIPTYNNYFIGNEPSKWASECRI
                     YQGITIQDVYPNVDVRYYTDRGTLKYDILVKPGGDVSKIALKYEGVDKLQVKNKELVI
                     STSLADFKESAPYTFQAGITGRKEISCKYVVKDNVMRFDVKDYDPTTTLVIDPQIIFC
                     SFSGSSSDNWGFTATYGPDESMFGGGIVFDGGGGFPTSPGAYQTNYQGGPFDIGIIKL
                     TPDGSNRVYATYIGGSGEDQPHSLFVDPLGNLVLAGRTNSGNYPVLNNGALAGSGYDI
                     VVTKLNAAGSALIGSRKIGGTGNDGVNITSNRGSATSLQRNYGDDGRSEVILDGSGNI
                     YVASSTQSNNFPGTAGFFQPTFGGGTQDGIVLKLSPDLNTLLFASYIGGSANDAAYVL
                     SLHPTTGNIYVAGGTESGDLLPNSQTGTIGPSIRGPIDGFLAIINNSGTNVIRSAYIG
                     TDGIDQVFGVQFDANGFPYIMGQTTGTWEILPVANPPYNNPGGKQFIAKLQPDLSAYV
                     YSTAFGSGGLTPNISPIAFLVDRCENVYISGWGGFFGTDNGYNSAGTNGLPVTPNAFK
                     SVTDGKDLYFFVLERNGVSQLFGSFFGENNRPGEGCDHVDGGTSRFDRNGKIYQAICG
                     NCKNLSAPPRPLYPVTAGAWSTVNNANTGGECNLTMLKIDMDLSGVRAGLQSSINGVP
                     RDTAGCVPLTVDFTDTIANAQSYEWNFGDGSPVVTTAIPSTSHTYNTVGTFTVMLIAI
                     DPTTCNIRDTSYVRIRVGNLEATLGFNPVKLPPCASFNYRFDNLSVAPPARPFGPQSF
                     IWDFGDGSPRVTAGPASVLHTYVAPGTYNVKLILQDTGYCNAPDSLVIPLNVADNVKA
                     QFIVPPTGCIPFDAVFENTSLAGQTFFWDFGDGNTSSVASPTHTYAIPGTYNIVLIAS
                     NPNTCNLSDTARFTIQVLDAPTPDFSFSPVSPAPNTPTTFNNLSSLDAVRFKWDFGDG
                     DTLLTTSRAPVLHQYNATGTFNACLTAYNAIGCPRTICREVQATIIPLVDVPNAFTPQ
                     SGDINSVVFVRGFGIAKMQFTIWNRWGQKVFETNDQNIGWDGKVKGVIQPMDVYAYTL
                     TVEFFDGVKTTKKGDITLIR"
     gene            complement(26421..28532)
                     /gene="recG"
                     /locus_tag="PLO70_01330"
     CDS             complement(26421..28532)
                     /gene="recG"
                     /locus_tag="PLO70_01330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQZ73124.1"
                     /translation="MSTSSHILSNPIEYLKGVGPQRADLLKKELNIFTFGDLLDHYPY
                     RHIDRTKINLISEITPQSEFVQVAGVLVNFDVVGQKAGRRIVAELKDKTGFLELTWFQ
                     GLSWVAKLLEAGQSYLVYGRVSFYNGKPQIVHPELEILSPEQQLAKNYLEPVYSTTEK
                     LKARGLGGRQIGKLTQLLMGMIREKDIEENLPSSVLEQLQLVNRFSAYKQIHFPQTNT
                     EYENAVKRLKFEELFIAQMRMNLLRSQRHRFSKGVIFERVGEYFNDLYRNYLPFELTG
                     AQKRVLKEIRADTAKGKQMNRLLQGDVGSGKTIVAVLTMLLAVDNGYQACLMAPTEIL
                     AQQHFQTIGLLLEKIGVTVKLLTGSTKSAGRKKTLQDLSEGYLQILIGTHAVIEDVVQ
                     FKKLGIVIIDEQHRFGVAQRARLWQKAEVPPHVLVMTATPIPRTLAMTAYGDLDYSVI
                     DELPPGRQPINTVHRYESHRSKVMDFIRSEIEKGRQVYIIFPLIEESSKMDYENLMKG
                     YENVKAYFPEPKYWISMVHGKQPAEQKELNMKRFVEGDTQIMVSTTVIEVGVNVPNAS
                     VMVIESAEKFGLSQLHQLRGRVGRGAEKSYCILLTGSKLGNDARERIRIMTSTNNGFE
                     IAEKDLELRGPGEIDGTRQSGALNFRLASIVQDRSILEIAKNIALELLENDPDLVSAE
                     NLQLKKFLHYKKGPVQWSKIS"
     gene            28993..30114
                     /locus_tag="PLO70_01335"
     CDS             28993..30114
                     /locus_tag="PLO70_01335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQZ73125.1"
                     /translation="MKNHIPLLQLDLYFSGYKFALLCFITAFVITLIAIPPIISLIKK
                     YRLYDVPNARKVHALPIPTMGGVAIVGGMMTALILWFPFSNQLAQVSFFFSVVVLFGL
                     GIMDDLKDLSAKYKFVIQLGLAALIALSGIRITSFEGLFGIEELPLTAQYTFTILAIV
                     GITNAFNLIDGIDGLAGGLGFMSLVTLGIFLTMSGDANTALIAFALAGGILAFLYFNF
                     NPARIFMGDTGSLVLGFVIAVLCIRLMQVSSLTGNPVLPHAPVFVLGIVLIPVFDTLR
                     VFMVRIWKGNSPFVADKTHIHHLLTNQGFSHAFASKLICFIHGFVLIEVYWLRLLKQE
                     IILLILTVIMLLAIVLLKNMGLVFRRYEKVNTLLKGDLS"
     gene            complement(30121..31020)
                     /locus_tag="PLO70_01340"
     CDS             complement(30121..31020)
                     /locus_tag="PLO70_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQZ73126.1"
                     /translation="MNYLKGLLRFQVIVTLCSVMLVRCSSGPTVSPVDEKGNTQTIFF
                     DDFSGPVLDSSKWNTEVTGMHVNNELQAYIDSTNVIRLVTGAEAEGAENGALVLQPYY
                     SPGYITKDSQKFDFISGRINTRSKVEFTYGSASARIKLTEGSGLWPAWWILGTGNWPE
                     TGEIDVMEYVGEKDWASSAVHGKGYSGDAGLVDRLYFPSGNDVTNWHIYAVDWTPDSL
                     IFKYDGVPMFRVTKPMTQFFGEWAFDNPKYLILNFAVGGVYPFKINGIKEPYYGLPAS
                     TFEQIKNGKSKMLVDWVRVMKVQ"
     gene            complement(31047..31865)
                     /locus_tag="PLO70_01345"
     CDS             complement(31047..31865)
                     /locus_tag="PLO70_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQZ73127.1"
                     /translation="MKIRRISTIYIVTMLCFMFIQKTGISQELFTKLVWSDEFEYKGL
                     PDSAKWGFDKGTGCPDICGWGNNELQFYTWNRPENARVENGKLIIETRKENFSGASYT
                     SARLVTKNKGDWKYGRFEIRAKLPAGKGLWPAIWMLPTNWVYGGWPKSGEIDIMENVG
                     YLPDSLFGAVHTEVYNGMLGTQKVNSTMRKDFSDSFHVYAIEWTAAQISFFVDGEKYH
                     TFKNEHSGSAAWPFDQQFYLLLNIAVGGNWGGKYGVDDRIFPQQLVIDYVRVYE"
     gene            complement(32108..33130)
                     /locus_tag="PLO70_01350"
     CDS             complement(32108..33130)
                     /locus_tag="PLO70_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HQZ73128.1"
                     /translation="MKYLPLFIFSVAVIVASCGKGDSPGNNNVAPTNLTVNAIVSTDN
                     SGNVSFTAAATNAVTYDYDYGNGVFQTVASGIVTYRYPASGTYTVNVIAKSASGQTIS
                     KSIQVTVTVALSLLWSDEFDTPGSPDPARWGYDLGAGGWGNAELQYYTNRTDNAIISN
                     GTLKIIAKAENFSGSAYTSARLLSKDKFSFKYGKVEARAKLPAGIGTWPAIWMLGNNI
                     NTVGWPACGEIDIMEHKGSQLNKIYGTVHHPGHSGGNADGGTVTIPNVTTEFHKYAVE
                     WSATTIKFSVDDVVFYTFANVSTLPFNQNFFIILNVAMGGTFGGPVDPGFTSAAMEID
                     YIRVYQ"
     gene            complement(33135..34034)
                     /locus_tag="PLO70_01355"
     CDS             complement(33135..34034)
                     /locus_tag="PLO70_01355"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73129.1"
                     /translation="MITNIKSIAVIFLAVLIGFSSCKKDKYSFGDIKTPADLTLTTVI
                     AGVDAANPNGNGTGIVTITTTATNALTYNIDFGDGITRVVPSGVVNYKYTNPGTYSYT
                     ITVNAVGTGGSLSTISKRITVFVAFEIPAGILQSLTNGSSKVWVTDNDAPGHFGVGPA
                     TEFSPIWYAAGPNTREPCAYDDEITFSKDVNNRVSMTIDNKGQSFSIGASTAFYGFSG
                     GDGCYAINTGGTRPLAFMDATSGSTSAVSTMIQFMVPGNGIINFGTGGTTYEILSITD
                     NSIHLRNIGIDGNSWYQKLKRKP"
     gene            complement(34053..36122)
                     /locus_tag="PLO70_01360"
     CDS             complement(34053..36122)
                     /locus_tag="PLO70_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73130.1"
                     /translation="MKSLKYFLGFAFLLVLAAGCKKETYDDVSFVDTANSPDQLSVLF
                     EITQDNTGRVTITPNGAGAVSYDIYYGDGTATPAKVQAGKNTIHTYAEGVYNVKIVAY
                     SINGKTTEITRQLTVSFRAPENLDVVINVDPANNYKINVSATALYETNFRIYFGDVPN
                     ETPVSFLEGATVSHTYAAVGTYTVRVVALSGGAATAEVTRSVTIVDPVLLPITFESPT
                     VDYTFGNFGGGNVTIINNPQSSGINTSSRVGRMVKNPPEVWGGSVITLGDPIDFSVNK
                     VFRMKVFSPRVGAKVLLKVENLTNGGISFEREATTTVANTWEDLVFNYAAINTAESYQ
                     KVVLIFELGIPGDGSANFTFLFDDIRLTNQLPTSLLTLPVTFDLPGVNYAVTDFGGNQ
                     SVDAVDPSNAANNVKKTTKPNGAETWAGTTIGSGFTSAIPFSATATQMSVRVYSPAAG
                     LPIRLKVEDRNDNTRSVETEKVTTVANAWETLVFDFANQATGTAALNLAYTYNMASIF
                     FNFGTAGDGKIFYWDDITFLPVNVAGIALPLDFESTTLVYTFTNFDGGDATVINNPQS
                     SGINTSSKVGRMIKNAGQVWGGSWIGLASPIDFSTLRTFKMKVFSPRVGAKVLLKVEN
                     QTNGGISYEKEVLTTTANAWEELTFDFSAINTANSYQKIVLIFELGTMGDGSPNFTFL
                     FDDIKLN"
     gene            complement(36148..>36803)
                     /locus_tag="PLO70_01365"
     CDS             complement(36148..>36803)
                     /locus_tag="PLO70_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ73131.1"
                     /translation="GLNGTGAMPYAAGWSTNLPTVNLANAYTAGDIRKAVTVLNIDDY
                     INNNPSLFGNLVASNNPAFALRPAYQVAPYKNTGLYNQKYLPRKGETSGQVELNYMNN
                     FRIIRYADVLLMAAEAFNKSTAPNDTKAQTYLNLVRRRAFGDNLHDISATGAALYQAI
                     LNERRLELAMEGDRFFDLVRTGQAASKITGFVAGKHEVFPIPQAEIDISGLAQNPGY"
     assembly_gap    36804..36903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36904..37859)
                     /locus_tag="PLO70_01370"
     CDS             complement(<36904..37859)
                     /locus_tag="PLO70_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ73132.1"
                     /translation="MNKNITIKIMAVVIATTLLSSCKKWVDYNPHEDFRITELDYLKS
                     ESDYRTMAISVYTPIQWINQAVPVGDIASDNAVSGGENASDVLSLQQIDDYTHTPVNS
                     TLTELWQAAYEGINRANYMHQYKAANLAGETVNFAGKDALYGEVYFLRAYYYFHLVRF
                     FGDVPMFVDRRLSLSESKSLDKTPKADIYKQIELDLTNAIAVLPAVQVQKGRITKYAA
                     QALLGKVYLYQNKFDLAASTLESVITSNAFSLVADFGSIFLATGENGPESVFEIQYSN
                     TSPYYNWGGVTRGQGNLAVQQCGIRGLNGTGAMPYAAGWSTN"
     gene            complement(37885..41010)
                     /locus_tag="PLO70_01375"
     CDS             complement(37885..41010)
                     /locus_tag="PLO70_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73133.1"
                     /translation="MKLKLALLSLSILVGGLLHQVMGQNLPVSGNVTNKTTGEPLVGA
                     TVLVKGGSANTITDQKGNFSITVPQKGSVLVISYTGMKVIEYKVADNTTAVAIQLEES
                     ATTTLTDVVVVGYGTQKITKVSGAISTIKSADIEKLRPVRTEEALQGRASGVNVIQSG
                     SPGSKPTVLVRGIPSFSGTDPVVIIDGVPQTLTDFNSINASDIESINVLKDAATTAIF
                     GVKGGNGVIVVTTKSGRKNQKTEITIGSNYGVQEVINTIGVLNASEYGAIINEGSTVA
                     GGNVIFPDLSVLGVGTDWQKEIFKTASLQSHTITARGGSDRMSYFLSGGYLDQGGIVG
                     GDDKSKFKRGNLTANLIFDVAPKLKLILNTTGVILNSRGIQENSFNSVLGSALNFDPT
                     VPKYNTVPNTVGQYGFSNLLVSEIFNPLTKLENTYNKNVGSKLYGKFELQYDVLKNLK
                     LSSRFGYTKYDGNAKSFTPLIFYGPNNVDNSMNADGSAVVGKHNSVAHEKTSNFNYTW
                     ESFANYNFNVKDDHTFETVLGFSLAKTTGNAAGATRQDVPFNSWEFADFTAATGNNSA
                     TNTNALAGYYYQYFRRNLSYFSRINYDYQEKYLASFTARRDGSYAFGKDNKYASFFSG
                     SLGWVVSSEDFFNSSFIDFLKIRGSYGSIGNENVNPQFVGIITGGPSYGNPPNSNGYT
                     FDNVFYPGSTVGSAANDALQWEKQLQANVGFDMTFFNRKFSLSADYFQRDVNGLLFTP
                     SASLYLGTVPIPTANIGSTKSSGIDLSLSYNETIGKSFKLNSFVTFTTAKSEVTATND
                     DGTAKILGGYYFNGQSQSVTVFEKGFTPGYFYGYKTDGLFQTADEVAAGPAQSGAQPG
                     DIRFVDMNKDGIINASDQTKIGDPFPDFTMGWNLNMEFKNFDFTAFTYASVGNDVYSA
                     YERNANFSNKYRGVLARWTGPGTTNDARNPRYSFTDANSNIRVSDRYVEDGSFIKLKN
                     IQLGYTFPASVTKKIFSRLRIYAQVKNAFTLTKYTGYDPEIAGGILDTGIDRGAYPQA
                     RTYSFGLDIKI"
     gene            41214..44114
                     /locus_tag="PLO70_01380"
     CDS             41214..44114
                     /locus_tag="PLO70_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HQZ73134.1"
                     /translation="MRKFALLFLFLPQIATGQNTIGLPDVINYPKQTYGAGLQSWDFK
                     QDKNGIIYIANNEGLLSFDGKYWKLYPLPNKTIVRSVEIGPDNKIYAGGQDEMGYFAP
                     AENGQLQYTSLTGLLGEKERSFGDVWDIASYKKDIFFRTPNRIFKLTNETISVFIAPS
                     EWTYLGACNDVLFAHDYKSGLLRFENNVWVPLPISNNLPVNDPVTAILPIHKDSSIIT
                     TLKNGLFVLSKTGFSKVTSPAITLIEKERIYAATALNADWFALATNNGGVHIIDAKGE
                     LIQNFTKTEGLQNNNVLSIFLDKQSNLWLGLDNGIDFIAYNSAIKRITPYAQDGSGYT
                     ALIHNNYLYTGTSGGLYSVPLQQMKDLSFSKGTFSTVSNTTGQTWGLSEINGRLLLGH
                     HEGAFIINENSAQPLLSNGAGFWNFVPLSSVFPSARTVAGNYKGLTFLNYNNNSFSIG
                     ETVPDFNESSRFVALDKYDNIWVSQPYHGLYKISIAGAGKYNVKPYTSKNGLPSTLNN
                     HVYKIKNEVVVATEKGVFIYNPSKDSFEPSPFYLNLLGPQSIRYLKEDTEGNIWFIHE
                     KNLGVVDMSAQQPAIIHLPELNNKMLSGFEFIYPVNDNNIFIGGERGFFHINYEKYKK
                     NIPALQAQVRAVKIVNKKDSLLFGGYFANVDDKQIQGKNQIPRISNNWKTIMFEFSSP
                     LFGQQANLEYTYKLKGFDENWAEWSGKTEKEYTHLAPGTYTFEIKVRNNLGNESEPVG
                     YTFKVLPPWYQTTLAYIIYLLLFGLAVYYLYKWQRKKFNQQQEKYEEEQKRLQYLYQL
                     EINKAENELVALRNEKLQSEIDFKNSELATTAMHLVQKGELLAKIKGELNQIMKVLDN
                     EKAVIELKKMIKVLSEDDKMDKDWEHFAQHFDKVNSDFVVSLKEKHPTITGNELKLCA
                     YLRMNLSTKEIAQLMNISVRGVEISRYRLRKKLHIPSEKSLFDYLINIQTKP"
     gene            complement(44122..45501)
                     /gene="mnmE"
                     /locus_tag="PLO70_01385"
     CDS             complement(44122..45501)
                     /gene="mnmE"
                     /locus_tag="PLO70_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQZ73135.1"
                     /translation="MNKLSGWDDTIVALATPPGVGAIGVIRLSGINAISIVNELFPSK
                     NLLEQGSHTLHVGFLKEGPDLLDETVVSIYKSPKSYTGENIVEISCHGSPYIQQQIIG
                     ACIKQGARLAKAGEFTQRAFLNGKLDLTQAEAVADLISSNTAASQKAALHTIRGGFSE
                     NLKALRERLLKFSALIELELDFSQEDVEFADRKQFYELINELKQSTDTLVNSFQLGNV
                     IKNGVSVAIVGKPNAGKSTLLNSLLNENRAIVSDIPGTTRDTIEEIINIDGILFRLID
                     TAGIRKQTNDKIENAGIERSLEKMKEADIVIYLFDVNNDKGEIERWKQEAEQKALKFL
                     LVANKIDELGEAIAKDKFGGIDNVIFISAKGNLHTPVLKEKMVDAVLQGKVQTENTII
                     TNARHYHALKEVSRSLTDIKQGLDNKLPGDLLALDIRRCLYYLGEITGEITNEDQLDY
                     IFSKFCIGK"
     gene            complement(45615..46445)
                     /locus_tag="PLO70_01390"
     CDS             complement(45615..46445)
                     /locus_tag="PLO70_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQZ73136.1"
                     /translation="MEANKILSADILDIIFDGKNKDYGAYELRKTYNRRITRALVITG
                     SVALLALLASVLSSTLSDKGDSKVKMNEMTIQDIKQEEKKEPPPPPPPPPPKQEPPKV
                     EMKQFTPPVIKKDEEVEKPPPPQEELKEAKIDVVNVEGVKDQGIVAPVEIDQGKQIVE
                     VKKEEDENKIFDKVEIEASFPGGDSKWRQYLERNANGQVATDNGAPEGTYTTLVQFVV
                     DKEGNISDVRALTNHGYGMEEEAIRVIKKGPKWTPAVQNGRQVKAYRKQPITFRVESE
                     "
     gene            complement(46502..47119)
                     /locus_tag="PLO70_01395"
     CDS             complement(46502..47119)
                     /locus_tag="PLO70_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQZ73137.1"
                     /translation="MASIDTGGGDGGHKKGPGVKKAKKLSTRVDMTPMVDLGFLLITF
                     FVFTATMSSPTTLDLNMPKDIKDQKEQTEVKESSVLSIMLGKDNQIFYYEGKLTEDGS
                     NFKSTTFKGIRDIIVNKKKEVMSRYVTDPECEAKARAKGRPISECADKDFVVIIKPSP
                     EATYKNTVDILDEMTINQVRTYAMVKIFDQEFELIKATEASSGIK"
     gene            complement(47141..47809)
                     /locus_tag="PLO70_01400"
     CDS             complement(47141..47809)
                     /locus_tag="PLO70_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQZ73138.1"
                     /translation="MPSVKIPRKSTDTDMTPFVDIAFLILSFFIMATKFKPPEPVEIT
                     TPGSVLSQKLPENNAVMISIDSSNRVFFSVLSEKDPGKYDAIINGINTSQNLGLSPAE
                     IANFKKTILVGVPFAGLKQLLGTNAKEQDKLKQPGIPVLDSANNQLVWWVREAKNAFA
                     GEKLNYLIKGDGKSKYPTFEAVIAALKRNEEFKYNLVTALDDIPPGTELYIKEKSGEK
                     DLKK"
     gene            complement(47873..48733)
                     /locus_tag="PLO70_01405"
     CDS             complement(47873..48733)
                     /locus_tag="PLO70_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQZ73139.1"
                     /translation="MAEIKPTATASVKATSVQAKKSSNAISWIAPIVCIIAGYAIWRF
                     MMGNPSGFSEPDAAGGFWPHHKGPKDAFHRIYEGGIIVPLLIGMLLMVIVFSIERFLT
                     IRKALGNGSIADFIRKVQYHLANRNVDAALSECDKQRGSVGNVMKAGLRRYKEMITEG
                     NLNTDQKVIAIQKEVEEATALELPMLQKNLVFLSTITSVGTLIALLGTVLGMIRSFAA
                     LGEEGGGGAAGDLAVGISEALYNTALGIGTSALALIMYNIFTTKIDSITYGIDESGFT
                     LTQSFASLYK"
     gene            complement(48958..50235)
                     /locus_tag="PLO70_01410"
     CDS             complement(48958..50235)
                     /locus_tag="PLO70_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73140.1"
                     /translation="MNFRDVFALAFRTIRSNKLRTGLTVSIIAFGIMALIGIITAIKA
                     MNQKFSESFSTMGANAFTVRFKERNIRFGGGNQREVKLTKKGKRKEKTSSLGKNITKD
                     EAELFVKRFDFPSVKSISIFGNRNNIVSHETYKTSPNVMMFGGDENYLALNGFALQYG
                     RNMNRMDVQTGRNVCVLGYDVADKLFKQGVGGAVNSVVRINNIPYRVLGVLESRGSTF
                     GFSRDNVIITTYSNIERNFPSGFSYVIAVMANELTKVNVAMGEAEGSFRAIRKLNITE
                     ENNFVLDRSDSVAEKAMNSLRFLTVSATVIGLITLIGAAIGLMNIMLVSVSERTREVG
                     LIKAIGGKSKMVSRQFLFEAIIISILGAVFGIVLGVIVGNMFSIVLKTGFIVPWDWVL
                     YGIIICTLVGLLAGLYPALKAGRLNPIEALRYE"
     gene            complement(50312..51685)
                     /locus_tag="PLO70_01415"
     CDS             complement(50312..51685)
                     /locus_tag="PLO70_01415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQZ73141.1"
                     /translation="MNALIISAILGVVMMFSGILLKQKTAVRNVAIGGIIILLLANIL
                     EMGGTVFFTIDSRGMIAFDQFALLFNTIVFVSTLLYLLLSAKEIEKVGVNYADYFALI
                     FFILCGITLVSSFKSLLILFLGIEIISIPLYILTGSDKRNLKSNEASLKYFLLGVFST
                     GLMLMGIALVYGARGTFITVPDPLVTAAPNTLLITGMSLLLFSMAFKVSAAPFHFWTP
                     DVYDGAPTVFTSFMATIVKAAVFIAFIRLFDDAFEGLQPQWRLWIAVITAITLFVGNI
                     TAVFQQSVKRMLAYSSIAQAGFMLLSLYAMNTEAKEGLLIYAAAYCLATIGIFAVLAK
                     MNDYTFDGFNGLARREPLIAATLTILLLSLAGIPLTAGFLAKFYMLKATIATPNSLWL
                     VIFAVLMAAVSVYYYFRVIQAMYFKEGNDATTGFSPFFKWTMVLMASLVILLGLFPDL
                     LLNWLYF"
     gene            complement(51698..53173)
                     /locus_tag="PLO70_01420"
     CDS             complement(51698..53173)
                     /locus_tag="PLO70_01420"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQZ73142.1"
                     /translation="MVAFFLIILPLLTGLIAFSLKEEKVVKSFAFLSSLATLAVSVLG
                     LTLMNAEENLQFTAQWMQPIGSSFSLKLDGMGQLLCLLTAISYPLVFLGSWNASYKKP
                     NNFFALMLLAQAGLMGVFLATDALVFYFFWELALIPMYFLSSQWGGEKRIPVTFKFFI
                     YTFVGSVLMLIGILYIQSKTVSHSFSLNAFYVVELSSKAQSWLFWLFFLAFAIKMPVF
                     PFHTWQPDTYEQSPTPTTMILSGVMVKMGLLGLLRWLLPVFPIASYVWGDVVMSLAIA
                     GIIYASLIAIRQDDIKRLVAYSSIAHIGLMCAAIFAENKSGMQGVMLQMFNHGINIIG
                     LWIIVEIIERRFGTRKMSELGGLAQKAPVMAIFFVIIALANIALPLTNAFAGEFLMFN
                     GLMNAKSNYYLWFTVLAGLGIILAAVYTLNMIRRIFYGEVNELTAVATDLKLNEKLAL
                     GIIVGLIFWMGVYPQVLLDVTDNFSQELVNKIDISHLFRKQ"
     gene            complement(53201..55108)
                     /gene="nuoL"
                     /locus_tag="PLO70_01425"
     CDS             complement(53201..55108)
                     /gene="nuoL"
                     /locus_tag="PLO70_01425"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQZ73143.1"
                     /translation="MQNALQIVYLIPLLPLIGFLINGLGRKHLSKSLVSVIGSGVILA
                     SFAMSIWVFMQVKGMTTPYVAHYFDFIKVGDLKIPFAFQVDQLSSLFLLIITGVGFLI
                     HVYSTSYMHKEKQEHFGRYFSYLNLFVFSMLLLVMGANYVIMFIGWEGVGLCSYLLIG
                     YWFKNTEYNKAANKAFIMNRIGDLAFLIAIFWMISRVGNVNFNDVFAVAMGEVDLRKF
                     TGTDITAITLLLFIGATGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIAR
                     SNVLYSMSSVTMDIVAYVGLATALLAATIALKQNDIKKVLAYSTVSQLGFMFLALGTG
                     AYVAAVFHVMTHAFFKALLFLGSGSVIHAMSGEQDIRNMGGLSTKMKITFVTFLIGCI
                     AIAGIPPFSGFFSKDAILLSAFEKSPVLYGLALFTALLTAFYMFRLLFLTFSGQFRGT
                     ADQKQHLHESPAAITIPLIVLAVLSIIGGFVGIPEVFINGGDRIGAFLSPVIKPHGGH
                     TVSHSTEYMLMGLSTALVIIMIIAAWFKFRNYQRTEEKGLGKVLENKWYVDELYDKVI
                     VQPMHQFGGFLKSVIEKSVIDGLVNGVGRAVNYGGRQLRLLQSGQVGSYILLMVLSMV
                     IIFALQFFLRK"
     gene            complement(55132..55470)
                     /gene="nuoK"
                     /locus_tag="PLO70_01430"
     CDS             complement(55132..55470)
                     /gene="nuoK"
                     /locus_tag="PLO70_01430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQZ73144.1"
                     /translation="MPINYYIFLSIALFSIGIVGVLTRRNAIIIFMCVELMLNAVNLM
                     LVAFSKMHYKAALLAGDVNGTAGTDGQLFVFFIMVVAAAEVTVGLAIIVMMYRNIHSV
                     DVNFLNRLKH"
     gene            complement(55472..55972)
                     /locus_tag="PLO70_01435"
     CDS             complement(55472..55972)
                     /locus_tag="PLO70_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQZ73145.1"
                     /translation="MNITQILFWFLSVVAIFSALMVITSKNPVYSVLWLIVTFFSISG
                     HYILLNAQFLAIVNIIVYAGAIMVLFLFVIMLMNLNNETEPQKHRWLKMAGAVAGGCL
                     LLVMVAALKDTDIKNQQAMVNEGTIGLIKNLGKELFTTYVVPFEISSILFLSAMVGAV
                     VIGKKE"
     gene            complement(55996..56535)
                     /gene="nuoI"
                     /locus_tag="PLO70_01440"
     CDS             complement(55996..56535)
                     /gene="nuoI"
                     /locus_tag="PLO70_01440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQZ73146.1"
                     /translation="MLTQRAKQVDRKPMTFLESIYLWNIVKGMGITISHLFKKKATIQ
                     YPEQKRTFSKVFRGLHVLNRDAEGRENCTACGLCAVACPAEAITMEAAERLPGEEHLY
                     REEKYAAKYEINMLRCIFCGLCEEACPKDAIYLSQTFAPANYGRKGFIYKKEELLIPH
                     PIKDKEAYEKALGEKAVKN"
     gene            complement(56640..57689)
                     /gene="nuoH"
                     /locus_tag="PLO70_01445"
     CDS             complement(56640..57689)
                     /gene="nuoH"
                     /locus_tag="PLO70_01445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQZ73147.1"
                     /translation="MYLLAIDLWFILEKAILIACIIGLSFFIAMYTTYAERKVAGWLQ
                     DRHGPNRAGPFGIFQPLADGGKLFFKEEIIPLASNRILFILGPGLAMVTALVTSTVIP
                     WGAPIVTDKGIIPLQVADVDMGILIVFSVVSVGVYGIMIGGWASNNKFSLIAAIRGAS
                     QMVSYELPMGLALIAVLFMTGSLKMSEIVANQAANGWHVIYQPLGFIIFFVCALAECN
                     RTPFDLPEAENELNFGYHQEYSSMKLGFYLFAEYINMFISSAVMATLFFGGYDIPFVN
                     EAAWGLEPWLLSLISFSVLMAKIAFFLFVFIWIRWTIPRFRYDQLMNLGWKKLIPLAL
                     LNMIISAAVMLWLNK"
     gene            complement(57699..58817)
                     /locus_tag="PLO70_01450"
     CDS             complement(57699..58817)
                     /locus_tag="PLO70_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQZ73148.1"
                     /translation="MADEVKKEAPANFKVTIDNITIEVAPGTTILHAARQIGGDVTPP
                     AMCYYSKLKGSGGKCRTCLVEVAAGSAADPRPMPKLVASCRTNVMDGMVVKNITSERV
                     QDARAGVVEFLLLNHPLDCPICDQAGECHLQDLSYEHGKEGTRFEFKKRIFEKEDIGP
                     YIQLHMTRCILCYRCTYVADQLTDNRVHGILDRGDHAEISTYISKAIDNDFSGNMIDV
                     CPVGALTDKTFRFKNRVWFTKPVDAHRDCDKCCGKVTLWQRGDEVLRVTARKDEWGEV
                     QSYDGKPGWICNTCRFDKKHINDWVIEGPTKINRHSVISQGHYLGLQIPKETMPDVMS
                     GREPKLLMDIHDVSEVNSVDISALPGPATSKTFEKKEQ"
     gene            complement(58843..60201)
                     /gene="nuoF"
                     /locus_tag="PLO70_01455"
     CDS             complement(58843..60201)
                     /gene="nuoF"
                     /locus_tag="PLO70_01455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQZ73149.1"
                     /translation="MGRKLLLDKAHIENIRLYDVYRREGGYRSVEKALKTMTPDAVTE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRYLVVNADESEPGTFKDRYLMEFIPHL
                     LIEGMITSSYALGSNRSYIYIRGEYAWIVDILEQAIAEAKNNGWLGKNILGSGFDCEI
                     YIQRGAGAYICGEETALLESLEGKRGNPRIKPPFPAVKGLWDCPTVVNNVETIAAVVP
                     IVNEGGEEYAKIGIGKSTGTKLISACGNIRKPGVYEIDMTISVEEFIYSDEYCGGIAN
                     GRKLKACIPGGSSVPILPANLLLTTAKGEKRIMNYESLADGGFATGSMMGSGGFIVLD
                     DSQCVVKHTYTLARFYRHESCGQCSPCREGTGWMEKILLNIEKGKGKMSDIDLLWDIQ
                     RKIEGNTICPLGDAAAWPVAAAIRHFRDEFEWHITNPDDAQRRNFGLAHYADPIYIPA
                     TA"
     gene            complement(60241..60660)
                     /locus_tag="PLO70_01460"
     CDS             complement(60241..60660)
                     /locus_tag="PLO70_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ73150.1"
                     /translation="MKKLLFIVLLLNGFFTNAQTVEEKLTLTLKEFHQALVNKNTVSI
                     NQQTDKWLSYGHSNGWVETKTDFIKNLETGYMNYNSYKEDSVQVVINGNVAHARFVAD
                     IAATLNGKEGNFHLKVLEVWVRKGKRWLLFARQAVKG"
     gene            complement(60664..61167)
                     /locus_tag="PLO70_01465"
     CDS             complement(60664..61167)
                     /locus_tag="PLO70_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQZ73151.1"
                     /translation="MNLRFSDEKLKEVQRIIERYPQGKQKSAVIPVLHLAQQEFGGWL
                     STETMDYVASVLSLKAIEVYEVATFYSMFNLKPVGKYMFEVCQTGPCMLNGSDDIVKY
                     IDEKLGIKPGETTADGMFTLKTVECLGACGYAPMMQQGKYYKEQLTKEKIDAIIAECR
                     NAAAAKN"
     gene            complement(61222..62445)
                     /locus_tag="PLO70_01470"
     CDS             complement(61222..62445)
                     /locus_tag="PLO70_01470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQZ73152.1"
                     /translation="MSEHHIKLPEGSIEKTTTTLNLGPTHPATHGVMQNILELDGERI
                     VSTEQTIGYIHRAFEKLAERRPLAQITTLTDRLNYCSSPLNNMGWHLTCEKLLGVKTP
                     KRVDYLRVIIMELARISDHLICNSIVGVDAGAYTGFLYVMQYRELIYEIYEEVCGARL
                     TTNIGRIGGFERNFNDIAFQKLEKFLREYPKVLKEFENLFQRNRIFMERTMGTGAISA
                     ERALNYGFTGPNLRAAGVDYDVRVHAPYSSYEDFQFNIPVGTTGDNYDRWLVREKEMW
                     ESLSIIEQAYRKVQDLKGAEAEVYHADVPEYYLPPKADVYTKMEALIWHFKIIMGETE
                     IPAGEVYNSIEAGNGELGFYLISDGGRTPYRLHFRRPCFVYYQAYEELIKGSMLSDAI
                     MTMSSLNLIAGELDA"
     gene            complement(62550..63056)
                     /locus_tag="PLO70_01475"
     CDS             complement(62550..63056)
                     /locus_tag="PLO70_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQZ73153.1"
                     /translation="MALTNETIKARLTEKFGDQLSHVEEPYGMLTFEAPKELNLKVLN
                     FLYDDEELKFRFLTDLTAVHYPDKKGRELAVVYHLHNMTDNIRIRFKVFTDISQPDIY
                     TATGLFSAANWMERETYDFFGINFVGHPNLKRILNVEEMDYFPLRKEFPLEDQTRIDK
                     DDEMFGRG"
     gene            complement(63094..63678)
                     /locus_tag="PLO70_01480"
     CDS             complement(63094..63678)
                     /locus_tag="PLO70_01480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HQZ73154.1"
                     /translation="MARPVSYNLIKKPLEGDISLVEMPDGSMGEGFFATSLDKVIGLA
                     RKNSIWPLPFATSCCGIEFMATMGSHYDLGRFGAERVGFSPRQCDLLMVMGTIAKKMG
                     PVVKQVYLQMAEPRWVMAVGACASSGGIFDTYSVLQGIDQVVPVDVYVPGCPPRPEAI
                     IDGVLRIQDLVGKESLRRRNGEHYKALLESYGIQ"
     gene            complement(63780..64175)
                     /locus_tag="PLO70_01485"
     CDS             complement(63780..64175)
                     /locus_tag="PLO70_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQZ73155.1"
                     /translation="MIHLLQTGSNTATAASYLPVAIQIAFAVGLVAFLMVVTHLLGPK
                     RKTSDKLQIFTSGIESHGDARQPMAIKYFLTAILFVLFDVEVIFFYPYAVNFKGLGWD
                     GFWAVLMFVGFFLSGFIYIIKKGALKWED"
     gene            complement(64328..66091)
                     /locus_tag="PLO70_01490"
     CDS             complement(64328..66091)
                     /locus_tag="PLO70_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73156.1"
                     /translation="MKKATITFLVPALLLISGLKAQTVQEGKNHLYAQRYNSAITTFE
                     KLLAANPNNIEAIYWLGQTYLESEEIMAARIASTRQLYEKAMQSTNSAPLIMVGLGHV
                     ELLENKGSDARQKFETALTMTRTKKGDNQEILNAVGRANTDAKGGDFNYAIEKLSAAA
                     DKGEKNAETFVLLGNAYRKARPGEGGGEAFRSYKKALEINSNFPLASLRLAKLFESQK
                     NWELVLQYLNEAITKDANYTAAYYDLFYYYFYRLDYAAAETQLQKYIDSKLPEKDIQD
                     EYLYAQLCWAQKDYTCATTRAESVVSSLGSKTKPKVYRLLSDAYFQKGDFAGAKKNSD
                     DFFAKKNPDDIILYDFQLRADILDKTGGTVDEIYNTYLQGAGIDTTLSLKIDLLKLGA
                     DRFKARGDSIGRIKEGDLRTVILKMKENPGQRDYFDAGFAYYQGKNYEKADSIFDVYV
                     EKFPDETYGYMMEYNIHRAMDSTMENGAAVPWAEKYLAILEKDTAKNKNYIMGVAGYL
                     AQYHANIAKDKIKALEYLRKMLALDPANTAIQQNISILEKASGNKPANPPSKGGPASG
                     KPATPKPSAKIVTSKNIVVRT"
     gene            complement(66134..>67035)
                     /locus_tag="PLO70_01495"
     CDS             complement(66134..>67035)
                     /locus_tag="PLO70_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQZ73157.1"
                     /translation="FPVFLLLFLLTACKSYEEQENELPDSRNRGTIRVSADETFKPVI
                     DEQVQVYESNHPGTSILVDYKPEAECLKDLFVDSVRMLIITRRASEEEKNFLSDSLRV
                     DAKSMIIARDGISVIVHPDSPDTIFTMKDIRDILTGRFKKNLIPVFDGVKATSTVRFI
                     IDSVLRGDSLTPNAMAARTSDAVVDYVAKNPGVIGFIGVGWIGNPEDSSQLSFLKKVR
                     VAHLESTDVKGSFVKAYQANIYLKRYPMVRDLVYILKEKHKGLGTGFANFMSGEIGQI
                     IFRRAYLAPAQKKLSIRPVKLNE"
BASE COUNT        20095 a        14809 c        12146 g        19885 t
ORIGIN      
        1 ggcaaaaaag atttattgaa tgaaataaaa gatttatccg ataaacagct tagctatgta
       61 tccccttcca ccggattatc tgtaaggaaa tgtatgttcc aggccgcaca gaccgagaag
      121 acaatgtggg aaatattaaa taacgctgtg aaaggtcccg ctaatcctga gaaacgatta
      181 ataataagtt ttaccgataa cgatttagtg gaaagggtaa aagaaggaaa gcttagtccc
      241 ccggttgata tcatatttaa acaggcaaat gcaccctgga ataatataac agcggctgtt
      301 actgatttta aagaccttcg aagtgaccgt attaaataca tgagaacatc tacagaagat
      361 ttgcgcaacc atgtgctgca aacatccttt ggctggatag attgctacca atatatcctg
      421 gtgatcacag cggaaacaaa ccgttacatt gagcagatca aggcgataaa gtctgagaaa
      481 aaatttcccc gaaaataatt accgggcctc aataattttt tcaagagaga tcattttacc
      541 tgacgtagtc attccctcca ccactaccct gaatttttta gtacggtcgc tgttataaaa
      601 actaactggt attacagggt tgacattatt gataaatata ttgggacgcc aatccagggt
      661 gatacgggaa tcacctttac tcagcagctc gggttctgcc ttatagtttg gtgcataaaa
      721 ttcttttaca acagaaaatc ctttatagct aattaaatct ccccttgatg agtttttaat
      781 atcctgtcct tttttagtat aaatcgccaa tgcacccccg gaaccacccc ccggagcagc
      841 agcaaagaaa ctgaagactt taatcattgc aatctgatta gcaggaatag tagccactac
      901 agccgaactt gtttcaattt cgtcgagata aattaccatt ggaatgttac ctaaagagct
      961 ggcagatggt ccctgcctgt aatagagccg ataaccggtg ggatcatcaa aaggattctc
     1021 caggctgggg agagatgatg tggaatagtc tggctctaca acagaaagac caggaaccct
     1081 cgactgcagg taatcaaaaa tattattttg aaccactata tcgctggtat tgaccatatc
     1141 aattgtccgt actgcttccc cactgaacag cccccttgta taccgttcat ttaactcatc
     1201 cgttgctgat ttctttttta cattaattat cacatcttca agcaatattc ctttttcttt
     1261 ttgtattgca tcaaaatcaa aagtcaattt attttgttca gcaggcgaaa cattttcttt
     1321 tgcagggaaa tgcactgtac ctacaggcag tgaaaaaatt tttgcagcag tatccccgct
     1381 tagctttaca tctataaact ggcttctttt gccccgtata tccaaaataa aaatcctgcc
     1441 attgccaaaa aagacaagtg agtcaagctt aaaaaacccg ttcttgtctg tattgatcat
     1501 ctgaaagttc ctgctcatcc cttctgcaga gataatagcc accattggtt tttcggcaaa
     1561 tggcctgtta cttccctgca tggtcacctt tccagtaatg gtgatataag aaggatcaga
     1621 ataatttagc gggtttttta ctttttgagc cagttcattc cagcgaaatc ttctccagcc
     1681 atttgtcatc atcaccaggt cgagtgccgt ttttacagaa tcttcatcag aagaaaaata
     1741 ccaggcgggg ttattgatat cccctctcag gtctgacgta agcaaaaaat tagaaaaaat
     1801 agtttcccgc ctgtatgttg ctaggctata atcagcatct gttatggaaa cagacaaatt
     1861 accctgcaca gtatctttca tgactatctt aaaatgattt cttcccttct ccaaaaaact
     1921 cagcgtatct gtacgcagat ctgctttctg gatatattct ttattattta caaaacataa
     1981 tctttctgct aatgggatac cttctttatt aaaaacggtt atttgaagaa tgcctgaatt
     2041 tagtttttct gtattcacta ctccctgtat ttcatttttt ccggaagcta attcctggcg
     2101 aaaaaccaca tggtgctgca tttgtccaat catataagcg gcccgcatgg aggggtcagt
     2161 aacccgttgc cttatttcaa agaaaattcc ttgtggatga ggaatcatag ttaaggtgac
     2221 accattggca gttatatcgg gcagagaata ttttttggca gtcacatctg cttcaaatga
     2281 agcaaaatag ttgaggcctg cctgcggtat tagttcaaac atacccatcc catcatgata
     2341 actgctgaat gaagtgattt tttcattttt gttattactg attgtacccc ttacagcgac
     2401 aggtattcct ttctcatctg ttgccttaaa ggcgatcgta ttcatcaatc cctccaccag
     2461 gttgcctccc tccggaaaaa attcaagttg aattgatttt tcatcctgct tgttggcaac
     2521 agcttgttta tctttttttc caaatatgaa aatgtccttt ttataaataa aatcaatacc
     2581 attattaagc atagtaggcg aataagtccg tatgaaataa gtaccggtaa tcagagagtc
     2641 cggcaattca atttgtccat tggtgcaacc aagcaaaaca ggaagaacct tgcggctaat
     2701 caacaccgat gattcgttca gcaattctac atacaataca gaacttattg tatcgggttg
     2761 ataatcggaa tataaatagg ctttaaacca ggctgtttct ccggcaagat aattgtcgcg
     2821 gtcaaaatgc agataagcct tttctatggg tgattgttta gctaattgcc cgagtaattc
     2881 ttcgggcttt tgtgcatcag cacctaagaa tatgcaaagt atacttagca gcagtaagct
     2941 tcttttcatc gattttgttt tcgctacagg tgtgaaatta gcttcaatat gctaaagtgc
     3001 aaaaggacaa agcgttaaca gtagtataat gtggtgcccc ggatgcaatt tacctgttgg
     3061 acaagctgta tatattgtct attttgtgtt gtcttaaccg gaaatatgaa aaaactaaca
     3121 tttatacttg ggcttgccat actgctgtct ggttgcaaaa aaaccgttga gcaaattcag
     3181 caggaccttg taattcaggc aatgacggat ggccagtggg ccatcaccag tttcaccgtt
     3241 aatggaacca acattaccat tgattttaca aattacagat ttaagtacta cagtaatagg
     3301 accgttgatg ctataaacag cggaactgtt gaaaaaacag gtacctggga tggtaatgcc
     3361 accacaatga ccacctcggc caattttaca gccgcttcgt atcctttgac cctgattaac
     3421 ggaagctggt taattaccag gaatagctgg acttacgtag aagctacaca aaccagtggt
     3481 gcagatatta agaccatgcg tctggaaaaa cagtaagcta tcttgtttac tttctgtcct
     3541 acctttacac cggctttgtt ttaaacatct taacctgatt cctgtcttat taacagagat
     3601 actatatttt gactagtaat ttttgtttcc aaaaaaagat tttaaggaga gaagatatca
     3661 gctattagga atagtttttg tgtaataaag ctaccagtaa aataaaccag ctacctgttg
     3721 aaaccactat atcacaatat tcccgggagg tttttcatct tttggttttt cattttcgga
     3781 gccatagctg cccggactca gcccgtcgca aactttactg cctctcagca ggttggttgt
     3841 tctcccatca ttgttaattt tcaggatcaa tctgctggca acccaacttc ctggctatgg
     3901 aattttggaa atggcaatac atctaccctt caaaatccaa cagcaagcta ttttacaccg
     3961 ggcacatata cagttacact gactgttagc aatgccggtg gcacaaatac tattgtacga
     4021 actgattata ttactgtcta tgaacctccc tccgtaaact tttctgctaa tattacaagc
     4081 ggctgtttcc cgctaagagt gcagtttact gatctctcca cagcaggttt gaacaatacc
     4141 aatgttacct gggactggga tttgggaaat ggtacaacat cttctttaca aaatccttcg
     4201 gtaacctata caaatgccgg aacttataca gtaacactaa gagtaacgaa cgacaaaggt
     4261 tgcacccgta ctatcagccg gccaaacttt attactgtca ctccaggtgt cagatcttcc
     4321 tttacacata cacaaccaac ggtatgcagt gccccggcta acatctcttt taccaatacc
     4381 tctacaggtc ccccaacttt gacttatcta tgggactttg gagatggcaa tacctccacc
     4441 ctgctaaacc cggtacatac atatacagta aacggaactt atactgccac attggtaacc
     4501 ttcagttctg ccggttgcca ggatactgcc agaagtaacc cgatcataat tggcggttat
     4561 aatacttctt tttcatatcc tgccagtgtt tgtataaatg aaattgtaaa cttcaccaac
     4621 acttccactc ctataccggt aaatgcaaat tggacatttg gggatggagg tactgccagc
     4681 gggataaata caacgtatag ttatcctgtg ccgggtgtat acaatgtcag gttatataat
     4741 acgtatggta actgtattga ttctgttgac cagaacatta ctgttaaccc aagaccagtc
     4801 gctgatttca tggcccccgt cacatccaga tgcgagcctc cacttactgt aaacttccag
     4861 gatctttcta ctggcggtgc agtagcatgg caatgggatt tcggagatgg caacacttct
     4921 actcaacaaa accccttgca tacatatacc aattacggca attttacagt aacccttatt
     4981 gccacaaacg tattcggatg caccgatact attacaagaa cagactatat aaggatacga
     5041 agggcggcaa ttagtattcc cgctttacct gcaagaggtt gtatcccctt taccatctcg
     5101 cctgttccag tcatttcagc attggatgct gttacctcct ttgaatggga ttttggcgac
     5161 ggtggtacat ccttcctgcc aaaccctacc catacatata ccgtacaggg aacatatacc
     5221 gtcaggttaa ttattacaac aagtactggt tgccgtgata ctttaaacat tccattttcc
     5281 gtacaggttg gcagcaaacc cgtagccaat ttttcagcta cccctaaccc ggtttgtgca
     5341 agaaaaccgg ttcagtttac tgacctgtca gtacctgcag atgaatggta ctgggatttt
     5401 ggagatggca gcacctccac tcttcagaat ccggtgcata attatacaga taccggttat
     5461 tttacgatac ggctaatcgc tcttaacaat ggatgtgccg atactattac tatacccaat
     5521 tatatatatg tgctgccacc aattgcccgg ttcacctata ctccagattg tggaaacagg
     5581 ctccgcttta gttttaccga tcaatccgtg gggccactta cctgggaatg ggattttggg
     5641 gatggcagtc cagtagttaa ccttcagaat cctgttcatc tttactcttc actgggaact
     5701 tatccggtac aattaattgt aacaaacggg ggatgcgcag atacgacaat acaaaccatt
     5761 aatgcggtag acgagagagc tgatttcact gctgatcaaa ccatcatttg caaaacggga
     5821 actgtcgtat ttacacctac tggttttaat ttattaaata tcacttctta cacttgggat
     5881 tttggtaacg gagtaacctc aacagtgagc aatcccgtta tttcaaatgc ctatcctaac
     5941 tcgggcactt ataccgttac gcttactatc acagatataa atgggtgtac aaatacaatt
     6001 gtaaaaaata actatatccg ggtaaatggt ccaaccgcaa attttagcgg aacaaatgtg
     6061 gccggttgta ccggccttac tgctacattc aatgatcttt ccactactga tggccagaat
     6121 aatattacca attggcaatg gcattttggt gatggtaata tccagaattt tagtgcccct
     6181 ccttttcagt acacttacaa tacaaccggt acctattctg taaaattggt tataacagat
     6241 gccagtggtt gcaaagacag cattacactt gctaacctgg taactgcaac agaccccatt
     6301 ccggattttg tggttgacac tttagcctgc cccggcgcta tactcacatt tacaaatacc
     6361 acatcgcctg caggactaaa cagtttctgg gattttggag atggcaatac atctgcgaca
     6421 ctttcaccaa cacatagtta tacggctgca gggctttaca cggtaaaact ccgggtacag
     6481 gatgtatatg gttgtgccga ttctataaca agaacaaact atatcagggt aggctctccc
     6541 gtgccagatt tcaatatcag cgattctatt tcgtcttgta cgccgcttga agtacagttt
     6601 accaatactt caacattcta taactcagta ctatgggatt ttggacctgg ccagggtacc
     6661 tcttctctaa tcaatcctgt tcactattat tctatacccg gcatttatcc tgtaaaacta
     6721 attgtcacca gtcccggagg gtgccaggat tcaattataa aaacaataag agtatacgat
     6781 acggcgggat caaggcttaa ttatattcct gtccagggtt gtaaaccatt ggtcatcgat
     6841 ctgaacgttc tgactagtgg accgatggaa tcctattttt gggattttgg tgatggttat
     6901 actcaaataa ctacggtacc caatgcaagc catgtttatt caacctttgg aaattttctg
     6961 cccaaagtaa ttttagagga cccctctggc tgcatcattc cgctacaggg ttttgatact
     7021 gcttttatta taggtgccgt agcaaagttt ggagtagaca ctactttctt ctgtgatttt
     7081 ggtactgtta attttttaga ctctaccact tttaatgacc ccattgatgt gtataattgg
     7141 gatttcggag atggaaacat ttctacatta caaaatccgg tacatcaata cagtagcccc
     7201 gggttataca cagtacaact aatggtacaa actcagcagg gttgccggga tacagttaca
     7261 aaaccggcac tcattaaagt tatacagcgc ccattaattg atataagtgg cgactcagtt
     7321 atttgtatca attcatccat tttgcattcc gggatattct tacagccgga tacttctgtg
     7381 gtcagctggc aatggacatt ccctaatggc aatacatcaa ccttgcaaaa cccaccgcaa
     7441 caaacttatt ctgtagcagg tagttttgtt gcaacagcta ttgcaacgaa tagcagcggt
     7501 tgtaaagaca ctacccggca aacaattatt gtaaatccaa tacctgtggt aactatgccg
     7561 gggcaaataa ctatacagtc cgggtttcct gttactattc ctgcgaccta ttcacccaat
     7621 actgtaagct ggatatggtc tccatcagca ggactaagtt gtgctaactg ccccacaccg
     7681 gatgcagttc caaaattcaa cacgacttac caggtttatt ttacagatga taataattgc
     7741 agtaatacag gaaaaatcca gattatcgtt atctgtaagg acggtaacct gtttattcct
     7801 aatacatttt caccaaatgg ggacggaagc aatgatgtct tttatccaag aggtaaagga
     7861 ctggaaagag ttaagatgtt acgcatcttc aatcgttggg gagaagtagt atttgagaaa
     7921 agagatttcc cggtgaatga tgcatcagca gggtggaatg gttcctataa ggggcaaaaa
     7981 ccaaaggctg acgtatacgt gtaccaggca gaagtttttt gtgataatgg tgatatcatc
     8041 cggctaaatg gaaatattgc attgatactg taactatgaa actgaagtta acgataaaat
     8101 atatcagctt gctttttaca gccttctttt cacagttggc agtatcgtca caggatattc
     8161 atttttctca attctttgaa gccccattgt ggagaaaccc ttccctggca ggtattttca
     8221 caggtgatgt aagggtgcag gccgtgtaca gggatcagtg gaacagtgtg acgaatgctt
     8281 acaaaaccgg ttccgtaaat gctgaatata aaatgcctgt tgggaaagta aacgactttg
     8341 ttactgtagg tatgcaggtt ttatatgaca gggctggtac tattggctgg acctcaacgc
     8401 atattttacc cgcattgaat tatcataaat ctttaagcac tgaaaataac cgttacctgt
     8461 cattgggttt tatgggtggc ctggtgcaac gaagatttga tccatcgaaa atgacgacca
     8521 atagtcaata tgataacgga ggactagggg aaaatttagc caatacaaaa tacagctatc
     8581 tggatggaag tgtcgggatg agttataatt caaatctcaa ttacaatcca aataataatt
     8641 tctatgtcgg cgttgcttat caccatttca acaggcccaa tacctcattt tatagaaatg
     8701 ccaatataga attatatcct aaatgggttt tctccggcgg cttacgtttt ggcgtatctg
     8761 attatgctta catgacatta caggctgacc aatccatgca aggtagtttt tctgaaacta
     8821 tggctggcgt catgtatggt ttaaaattag gtgaagaccc ggataatccg gcatacacta
     8881 tacatgctgg tggttttttg cgttggaatg atgctttgat accagtgata aaattagatt
     8941 ataatccttt ttctgttgca ctcagttatg atgtcaacat ttcaaaattg aagccttcaa
     9001 gctatgggcg gggaggtttt gaactctctg tttcttatat cggtttcctg aaaagaaatg
     9061 aaggttatct tctttgtccg aaattctgac ttatctctta gtaaaaaaaa gagcaatcat
     9121 tacaattgct cttcctgtct tattgtatta aaaattactc ccctttcgtt tcaaacaatc
     9181 ccttccaaac caatgccgcc gctactcctc caacaatagg ggcaactata aaaagccata
     9241 actgggttaa tgcttttccc tgtgcaaaaa cggccgggcc aaagctacgg gcaggattga
     9301 cagatgtacc tgtaaccgga atagcaacaa gatggatcaa caccagtgtt aagcctattg
     9361 aaaggcccgc catggtacca ttgccccatt ttgaagtagt gccaaaaatt acaaacagga
     9421 aaaggaaagt cagtacggct tccgtaataa aagcagaagt catattgtaa ccgccaagat
     9481 aaccttctcc ccaaccgttg gatccaagag cccattcgcc cattgaaaaa ccgggtgaac
     9541 ctttctgtat tacatataat actcccgctg ccagcgttgc accggttaat tgcccaagaa
     9601 tgtattgtat tgcttctcct gctttaattt ttcctgcaac caacatagag attgtaatcg
     9661 ccgggttaat atggcagcct gaaataccgc ctattgcata agccattgct accacagcga
     9721 ggccgaaagc aaaagaaatt cccagcaaac caattccgga aggtccggat gtggataccc
     9781 cggaaactac cgctgcaccg catccaaaaa gaaccaggga gaatgttccg attaattctg
     9841 ctaaaagctt tttcataaat aaattgtttt ggttgaaaaa aagaaactac tgacatgaac
     9901 cattttactt attgtttgtt tctgtgaggg taaacgaata taaatatttt tactttcatt
     9961 taattcaaaa taatgagtat ccaaattggt caggctgcac cagattttac tttattcgat
    10021 agcgataaaa acccggtaaa gctttctgaa caaaaaggca acaatgtcct tttgctgttt
    10081 ttcccgcaag catttaccag tgtctgtaca aaagaacttt gcgctgttcg tgataatatt
    10141 gcactttata ataatgccaa tgccaaagtg tttggcatat cagtagactc ggtatttaca
    10201 ttggccaaat tcaaagaaga acaacaattc aactttccac tcctcagtga tttcaataaa
    10261 gaggtttccg ggaactttga aactatttac aaagattgga ttctgaacat gaaaggtgtg
    10321 tccaagcgat cagcttttgt aattgacaaa aaaggaaata ttcaatacgc agaagtactg
    10381 gaaagcgccg gcgatgtacc tgattttgaa aagattaatg ccgtgctaaa taacctctga
    10441 taagacttcg ttaaaattgc ttgatctgtc aactctaagt cattgattaa gagcatgtaa
    10501 aaccatatcg tagttttacg ttgcacagat aaaaaattag gcctattttt cgaataataa
    10561 aagtaaattt gtttcgttga gacggattct acccatatta tcctttattc tgttgattgc
    10621 actcaattca gcgggtcagc cttcaagaac aaatccttct ggtgatggag acaggattgt
    10681 aaagttatac cccaatccag ctacatcgta tattactttt gatttacaga ataattttag
    10741 aaaaggacta actgttcaga tctataacgg agtgttaggc aagaaaatgt tcgaaacaca
    10801 aaatattcca gataaattta ctattaacct gaatgacttt attcgtggta tttacattta
    10861 tcaccttgta gatatgaccg gcaaaatcat tgagtccggt aagttccagg tttcccgtta
    10921 aatactttgt ttctattttt tattgtacct gcactataag aaatttcagg tattgagtat
    10981 tgtccatacc ccagataatg ggatggtctg atgactgtgt ttgaaaagtc acttgtctta
    11041 tttttcttct cacatctttg gccgccattt ctattgtttg taaaaacaat tcaggattaa
    11101 caagattggt acaggaagat gttaccaaaa agcctcctgg ctttaccaat ttcatccccc
    11161 gaagattaat ctccttataa ccagttattg ctttttgtat cgtttcccgg ctcttcgtaa
    11221 aagccggcgg atcaagcatt actacatcat attgtcttcc gtcttttccc cactgtttta
    11281 aaacatcaaa ggcattggca gtttcaaacc tgcacctgtc ctgcactcca ttcaattcag
    11341 catttctatt ggcctgggct actgcatttt ctgaaatatc aagccctaaa acagattttg
    11401 ctccatactg tgcagcatgt atttcaaaag tgccagtata ggtaaaagca ccgagcacat
    11461 ccgccccttt tacaatatgc tggattgctc tccggttatc ctgctggtcc agaaaataac
    11521 ctgtcttctg accattttca acatcaacat gaaacttcaa gccattctcg ttgataataa
    11581 ttttcgtatc aaatggtgct gataaaaatc ccttttgttg tggcaatccc tccaactctc
    11641 ttaccggaac atcattgcgt tcataaatac catgaggttg aaaaattgta ttgagggcat
    11701 ttacaatggc cggtttccag acatcaatcc ccagggccag tgtctgaata acaaaataat
    11761 cattgaactt atcgatgata agctggggca atgaatctgc ttctccaaaa acaagccggc
    11821 agttttctgt atatccaatt cttttacggt attcccagca tttactaatt tgttcaatga
    11881 aatattgttc atttatttca tctgttctgg ttctggtcag cagcctaacc agtatttgtg
    11941 atttaggatt aatataccct ttcccgataa attttttatc atgatctaac acttctacca
    12001 catccccacc ctgtacatca ccctctatct tctccacttc attattgaat atccaggggt
    12061 ggccattggc aacccggaga gagatttttc ttttcaggta aactttgttc ataaagcggc
    12121 gaagatagag gaaagaagta taagaccggg aatcaggcag ttggtacaaa aggtgaaaaa
    12181 aaaccttcca aaccccctga cgttcagact ccctgaccct caattacagg tcattttgtc
    12241 ccaaatccac ggttggcaat attcttgaca accttacctt tgcaaacaat ttgtacaaaa
    12301 atggcagaaa aagatatgaa gcagcaggaa agcacacttc cggataataa ttcaggactt
    12361 gatatcaaca cagatgaaaa tcttagtggc actacgcact taaatgagcc ggtatcagag
    12421 gaatctgagt tagagaaact tagaacagaa ttggaagaat caaaggacaa atacctgaga
    12481 ttagtagcag agtttgataa ttttaaacgg cgaaatgcca aagaaagaat cgagctgata
    12541 caaactgccg gaaaagaagt gataactgat ttgttagatg tattggatga ttgcgacagg
    12601 gctcaaaaac agttagaaac aaataataat tctgaagcga tcaaagaagg tgtattatta
    12661 gtatttaata aacttcgaag cactttgcag tcaaggggtg ctaaaccaat ggaaaccctg
    12721 aataaagaat ttaatcctga cctacacgaa gctattactg aaatacctgc tcctgcagca
    12781 gatatgaaag gtaaagtagt ggacgaagtg gtaaaaggat attacctcaa tgataaaatc
    12841 atccgccatg caaaagtggt cgtaggaaag tagagaaaca gtttattaat taattggtta
    12901 atacggcaaa tgtcaaaaag agattattac gaaatactgg gagtaagcaa aacagcatct
    12961 gctgatgaaa ttaagaaagc ataccgtaag gttgcaatgc aatatcatcc tgacaggaac
    13021 cctggagata aagcggcgga agaaaaattt aaagaagcgg cagaagcata tgaggtatta
    13081 agcgatacag ataaaaaagc tcaatatgac cgttatggtc atgcaggtgt gacgggcaac
    13141 ggccggggtg gttttggtgg aggtggcgca ggtatgaata tggatgatat tttcagccag
    13201 ttcggagata ttttcggaga agatcttttt ggaaatttct ttggtggcgc ccaacgggga
    13261 ggccgcggcg gacaaagaag tcgcggtgta agaggaagta atcttcgtgt caaattaaaa
    13321 ctgacgtatg aagagattgc caaaggcgtt acaaagaata tcaaagttaa aaagcatgtt
    13381 atctgctctg cctgcagtgg cagcggggcc aaagataaag gtagtgtgca aacctgtggc
    13441 acttgtggtg gtagcgggca ggtaaggcgg gtgcaaaata cgtttctggg acaaatgcaa
    13501 actgtaacta cttgtcccac ttgtaatggg gaaggaacaa cggtaactgc aaaatgtacc
    13561 agttgtaaag gtgaaggccg tgtatatggt gaggaaacag tgagtattga tataccagca
    13621 ggtgttcagg aaggtatgca gatgaatgta aatgggaaag gcaatgcagg agaaagagga
    13681 ggcatgccgg gtgatctcat tattcttata gaagaagaac cgcataaaga attacatcgt
    13741 gaaggattga atgttgctta cgaactgcat ctttcattta ctgatgccgt cttcggcaca
    13801 caggtagaag tgcccacaat tgatggcagg gccaaaataa aaataccccc cggcacacaa
    13861 agcggaaaga tattccggtt aaaaggaaaa ggatttcctg ctgtaaactc ctacgaaaag
    13921 ggtgatcagc ttatacaagt aagtgtgtgg acacctcagc atctgagtac tgaagaaaaa
    13981 gctgcactgg aaaaacttag tcattcccct aacttcaagc ctcatccaga taagaatgac
    14041 aaaaatttct ttgataaaat aaaagaaatg ttttcttaaa tcaccagttt gctcctccgc
    14101 tttttttctt tttcactttt gaataaagtg ttttcgcctg ttgaaccagc gttatgaact
    14161 ccttttgcag gagatcattg ttccctgagg atgattgtgc cagtgttacc acctcgttcc
    14221 agccaatatt tttaacaaat ggagaactgc ggagcaaaga accaaacata gctactgatg
    14281 ctgaaaaatg atagcactta tctatttctg caaatggtat aaaatcaaag cggcttgtat
    14341 aattgaaata atgatgtgta gtatcatttg gatggcggta ttgtaattta atatcagcca
    14401 gtttgccggt agtaaaatta tccttaatag cacctttatt aatttcagta ggcactagct
    14461 cgaacatcgc aatcatagaa tgaccagagc caatttctcc tccttcgatg attgataaag
    14521 aatcccgtaa agccccgacc ttattatcaa aacccaccag gcggtaatct ttcacatatt
    14581 cgggattgaa ctctacattc atgtacacat catcggctac cgaataaagt gtctgtgtaa
    14641 attctttcag taatactttc tctgcctcct taaaattgtc gaggtaagca aaattgccat
    14701 tccctttttc tgccaacagg tggatttttg aatctttata gttgcccata cccacaccca
    14761 aacaggttag ataaacacct gattcccgct gagcagtgat catttcatcc agttccgttt
    14821 ccgtttttaa gccaacatta aaatcaccat ccgtggcaag aattacccgg ttgtttcctc
    14881 ctttaataaa atgattcctt gccacactat acgccagttt tatacccgat tcacccggtg
    14941 tagagccacc aggtgtcaat tcatcaatgg ctttcagaat gatttccttt tcaccaccac
    15001 tggtggtatt taacatgaca cccgtaactc caccatagac cacaattgca actgaatctt
    15061 tgtcccgaag attatttacc aataaccgaa aagctgattg cagtaaaggc aaccggtttg
    15121 gcatatccat ggaacccgat acatcaatta aaaaaacaag atgacttggt ggcaatgtat
    15181 ccaggttcag ttttctggat gagagatcaa tataaaataa ttgattatcc tgattccagg
    15241 ggcagctgct taatgtagtt tttaaatgaa aaagttcatt ggcagctggt tcatgataat
    15301 ttagattaaa ataattgagc atttcctcta tacgcactgc atcaggggga accattgagt
    15361 tgagtgtaat gaacctgcgg atattactat atgaagctct gtcgacattt aaagacatac
    15421 cggtattggg aaaccgttta gcattcacaa aatgattctc cagtaagctt gcataagttt
    15481 catcaccagt aaaccatttt ttttgctcct cttttccaag gtctttggta aaggaggata
    15541 atttatctct tctggaatta gaaaccgatg ccgggagtaa cctgagtttg acatttacat
    15601 aatgttcagc atcaactacc aaagtctctg tacgataccc caccagcgag aagctgaatg
    15661 tatctacctg atgatttgcc acaatgccaa atgtacccgc cgaaccactt ctgaaaatat
    15721 aaccagtctt atattgcctg attgtaacat tttgcaaaat attccctgat tcatccttta
    15781 cctcgccgcg aacataatac tgttgtgcgt ataaggagaa actaaagatc agaaagacta
    15841 atatatgaca agccgccttc aattggatac attggtcgat aaaatacaaa aaaagcagga
    15901 gagatttcct gaaatcatac cagctggtaa atctctttca aattacgccc cagaccatca
    15961 taatcaagtc cataccctac aacaaactta ttagggatat caaatcccac ataatccagt
    16021 actaagggat attcaatagc ctcagattta tacagcaatg tcgcaatttt cattgactta
    16081 ggctgttgat gtaacagttt gggcaggaaa ttatgaagtg tttttcccgt gtctacaatg
    16141 tcttctacaa ttataacttc ttttccgaac aggtcatctt ccaggccgat tgaagtaacc
    16201 acattaccgg atgatttcat gcctttatag gaagccagtt ttataaaaca aatctctgct
    16261 tcaatagtaa gatgtttgaa taaatcagct gcaaacatga aggagccatt caatatggct
    16321 ataaatagtg gctttttccc ggcataatct ttatttatta tgccagccag ttcttttatt
    16381 ctttgttgta tagtttcggc tggcagatac gtctcaaatg atttatcatg tacccggata
    16441 attgccatac tgattattta agcgtttagt tggttgcctg tattcttctg ccggaattca
    16501 gggaaaaatt ttcctttatt gccaatttag gcattgctga cgatttcttt ttcaaactta
    16561 attgctcccc cggagccggc agctcattgt tctttaacca atttaattcc aataagcttt
    16621 ccaatcttat accctgtttt tgtgcaatct catgcaggtt ctcttccggt tgaactatat
    16681 aaaattcttc acttcctgtt ttacgttttc gttgtaaata gatcaactga tctttttccg
    16741 aagcctctga ctgggaaatc tcattgaatt caaatagcct cgccagcggt acattgtatt
    16801 gctgagcgat agaaagaaaa gacgttcctt gttgaatgta taccacccgg gtatcattta
    16861 tcctgaactc ccctttcggg tattccgatt tttttacaga ttgattttcc cctgcaaccg
    16921 ttatagccgc ggaaggagtt gaaggaatgg taatttcttt attcatttca ggtatcgaag
    16981 ccatttcgtc cggctttgcc tgctgttgcc ctagtgcgat cagggtatat tcctgaaggt
    17041 tataatcttc aatcaatttt atcaatacct gcggatactt tggatttgta gcgtagccag
    17101 cattctttaa accattggcc cagccggtgt aatctgttgg atccaggata aaaagtgagg
    17161 cgtagcgctg gctgtttttt aaaaaattgg aatgatcacg gtatgaatct tccggagaag
    17221 aatatttcct gaaacactcc cctcttgcat catcatcatg actaactgat tccccggtcc
    17281 agttagattt acatttgatc ccaaaatgat tatttgattt caacacaagc ttgctcgtac
    17341 ctgccatcgt ttcatgaata ccttgtgcca gtttaattga tgccggcacc ccggttcgtt
    17401 gcatttctgc catggccaag ctcttataaa tattgatata gttcttcact acttctgagt
    17461 tctgtgcagc agcagtaaaa gcagtaagcg ataaaaagaa tatcagaatt attcttacgt
    17521 tttgcatggg atagttcgtt attacagttt ccgtatcagg aatagaccat cacggatagt
    17581 taatatcact ttttctatat ccgttcttcc ttttataaat tcattaaagg cctttatacc
    17641 ttttgcactt ttacccttaa cattctcctc cagtacctgg ccatgaaaaa agatattatc
    17701 tgctaaaata aacccgtttt tccgcaaccg gggaaaaaca aggttaaaat attcaatata
    17761 agccggctta tcagcatcta taaacaccag gtcccaggtt tcttccaacc ggggaatgat
    17821 atctaacgct gaacccgtat gagatattat ctggccagca tgagcagacc ggtcaaaaaa
    17881 atcccttgcc cgggctgcat ctttttccct gagctcaatg gtatgtaaaa gaccatcctc
    17941 agttagtccc ttggccaggc acaaagcact ataccctgta aaagtaccaa tctcaagaat
    18001 tctcccgggc ctgatcatgc tactgatcat ctccagcact ttaccctgca agtgcccgct
    18061 cagcatgatg gactcggaat gattttgcag tgtaaactca ttcacctctt tcagcaaaat
    18121 atcttcagcg gaagaatatt tttctgcgta tttctcaact aatggattga taagttccaa
    18181 tctttgaaat ttcctgcaaa gataaactta tacagcttgc aactttagtt ttgacagcga
    18241 tggtttttta gaaataaacc atagacctaa gaacgtgaag atgccattta tgaggatcag
    18301 ctcattccca attttatacc cattaaatat tttattagaa agatcctgca atccgagtga
    18361 taggtgcaat ttcgcatcgt accaggccgg gttactaagc atgtctacag aaagtgccag
    18421 taaaggtgca acaatacaaa ctgtccagat gagcttatta tttaactccc gtttagttaa
    18481 tataccgaaa gcaaacagcc cgagtaatgg cccgtaagta ataccggcaa attttaatat
    18541 aaagtcgatg ataagcttat cattaatcca cttgaatact aataccatga taaaaaatac
    18601 caatgcaaaa gtaaaatgca cctttaacct tgttctcttt ttttcctttt cggttcctgc
    18661 cattttattg ataccgagaa tatcaatgca gaaacaggat gtaagagaag taatggcccc
    18721 atcaacactt gggaataatg ccgagatcaa tgcaatgata aaaattatac ctatcactcc
    18781 gctgaaagta tcactcaatg caattgttgg aaacagatca tctggtggaa cattaattcc
    18841 tttggtaccg gcatagaggt aaagtacacc accaagaaca aggaaaagaa aattaactac
    18901 catcaatacc gcggtaaagg tcattatatt tttctgagaa tctttcagtg ttttcacact
    18961 gatatttttt tgcatcattt cctggtctag tcctgtcata gcaatagcaa taaacatacc
    19021 cccgactata tgtttccaga cataagaccc ggcttttaca tcccagttaa agatttgtgt
    19081 atatccttta tctgccatca ttgacattgc tccgccaaaa tcagttccca gggctttaat
    19141 tattactatg atgcaggcaa ccagtccaac cagcattcct gtcgtttgaa gggtatccgt
    19201 ataaattatt gtctttactc ccccttcaaa agtgtacaat agaatcaata acagaataac
    19261 gagggctgtt gcttcaaaag gaatgcccag tctgttaaaa ataaaaatct gcaatacact
    19321 tattaccagg tacaatcttg cggtagctcc gactgtacgg gataaaataa agaaaaaagc
    19381 tcctgcctta tgcgcattaa tacctaatcg tctttcgaga taggtataga tactcgtcag
    19441 gttcatccgg taataaaggg ggattaacac aaacgctata accatatagc ctaacagata
    19501 acccagcaca acctggtaat agtaaaaatt acctgctcca acaccacccg gcacactcac
    19561 aaaagtaaca ccggacaaag aagtgccgat cataccaaat gccaccagca tccagttgct
    19621 gttacggtta ccgataaaaa aagattcatt atcagaattg cgggatgtgt accatgctac
    19681 tcccagtaac aaaacaaaat agccgataac aaatgagaaa agtacaatgg gtgacataca
    19741 atctggtttc tgttaatcaa tagttatcaa tacaaaatac gaataatgaa ttttgaagat
    19801 aagcaaaaaa gttatccctt tgctgcaata tactatgtat ggcgattcaa tctcttgcag
    19861 ttttctgcgg ttccaaaaat ggaactaatc cggtatttgg gcaacatgca tctcaactgg
    19921 ggaaactaat ggctgaaaac aatatccggc ttatttatgg tggtggcagt gcaggtatta
    19981 tgggcaacat tgccgataat gtgatgcagc atggcggaaa agttactggt attattccca
    20041 aattattatt ggaatgggaa gttcagcacc ggggtatcac agagttaatt atctgtgatg
    20101 atatgcatga aagaaaaaaa aagctttaca gtctttgcga tgcagcattg atccttcccg
    20161 gtggttttgg cacattggat gagctatttg aaatagtaac gtggaaccag cttacaatcc
    20221 atgataaaga aatttatatt ctgaattcag gcggattcta taaccacctg attaaacata
    20281 ttgaaataat gaaagcagag caattcctgt atgaagaggc aataaaaagg atcaccataa
    20341 ttgatgaccc ctctgaactt acccgttatt tataatcaga attaccgtct ccattgatca
    20401 ccctggaata atggcacttg tatgccaccc ctgattatag gtatggttct tccatatgca
    20461 tcggtatagg gggaattatc atttccagac acatcaaata atacagcgag gtaataaaaa
    20521 ggctgaccgc cttttccctg acggcctgtg gtgtaaccac caccaaccag catactattg
    20581 ccggaagtgc tgtaagtttg cgtagcaccg ctgttcggaa taaatttctg cctgataaaa
    20641 ttatgttcaa actgggcctg tgcaaacaaa aaccgcaggg gatacagttt cacaaatgca
    20701 ccacctccat atactgtctg tcgtaactta tcattaaaac cgtaattgta atcccggtag
    20761 gaggtgtagt tgtaattcac cacaatacca gcatcagccc aattgctgag gctataacca
    20821 aacacggggc ttccgccgat caggaatgtg ttattgaaaa acgacaatga aatgcttcca
    20881 ccggtaaaaa gattctcttt aaaacccggc ttcgtttctt cctcttcatt ttgcgcaaac
    20941 gatactgctg cagaagcaat aaaggccaat agtaagaatg tacgtttcat attacggtta
    21001 ttttaatgat aattattcaa actcctgttg ttctttccaa tcaagcgcaa gctcattaac
    21061 tacttttttg gtgatgggta cttttgataa catcaatgtc aatagtattg ctgtaatgat
    21121 cagcagggta aaaatcccgg ttagaaaaaa aacaactaca gacaacaatg ctgctgcttc
    21181 gcacaaagcc tggtataaga ttaatgccga acgatattgg tttaatttat cacttaagga
    21241 ttttccggtg ttctttattt ccgacacctt cctcttatac aagtattttg caacaattat
    21301 gcagatggac gcaaccacca caactatcca cagaaatagg gtttcgtaat ttgatcctgc
    21361 ctgaccctgg acaggcccat taagttgatt aattactact gctatcagtg caaaaaaaat
    21421 tactcctgtt gacagtgctg ctgtcaaaat cctcattgcc ctgaggtttt ctttcggcga
    21481 acctgtaaat tcaatttgct ttctcatcaa ctaaaaaata ttacacagtc aatttctggc
    21541 ttgcagatga tcacttaata atttatgatc cataaaattg taaaaatcgg aataaacagg
    21601 gaaattctga aaagcagcaa attaatactt aaatgacgga cacattgatt cccgtttatt
    21661 tccgcggtag gcgtctcttg aaccttgctt aatcagcgaa aattcgaatg ccccgcgggt
    21721 attgttgaaa ttctttaaag aagaagtggt tgcgtcatac gtaaaggtaa aagtgatatc
    21781 ttttataccc agtcctacca tcgggataac ggcatcttta taacgataat aagcacctgc
    21841 catcagtatt ttgtcgccgt ctcctgataa attataatgc gcatttaatc cacctactat
    21901 ttcgtaggat tttgcctgta aagaaaaata cgcattggga ttaacgatga ccatgtcatt
    21961 taacttgaaa cttccattaa taaaccccgt ataacgaaca ggtattcgat tatcgattcc
    22021 tggctgttca ttaaaaaatg attctcttgg ttgattgata tgcatggcag agaagcccgc
    22081 attaagatag gtactggtat ttggaaaata agcatagttc atacccacct gcatatcgat
    22141 gtaattgata ttgttataat ccagtttggg agccgccgtg gtctggtgag catcaaaaaa
    22201 ctggccgttc cactggctgg gaaaactaag attcgatgtg ttgatattct tattggccca
    22261 tcctgcatta aacccaaggc tgaccaggct tccggcattg atcatctggt ggtaggcaac
    22321 agagccatac acttttgtgg aagtaaggac actggaacct gctacatcac gcaggatcac
    22381 accaccaagg cccagccagc cggtattctc actgttctgc attaactgaa catcaccaaa
    22441 tgcactcatt gttttataag gaaaggcagt aatagaggcc cattgattac gataatttac
    22501 gccaagcctg taatcgccat caggtataaa tcctgtattg gctggattag ttagcaaagg
    22561 agaattcatg aattgtgaaa aatgaagatc ctgtgagtga gcattaaggc ccaaaccaca
    22621 aacagccagc aaaattatat ttctaatatt attcatttcc ggataatcaa attagcacat
    22681 tttcaaatct tcctacctga ttaacgttat atcacccttt ttagttgttt ttactccatc
    22741 aaagaattca actgtcaatg tataagcata cacatccatc ggttgaatca ctcctttaac
    22801 ttttccgtcc catccgatat tttggtcatt ggtctcaaaa accttttgcc cccagcgatt
    22861 ccagatggta aactgcattt tagcgatacc aaagccacgg acaaatacaa cgctattaat
    22921 atctccactt tgtggagtaa atgcatttgg aacatctacc aacggtataa ttgttgcctg
    22981 cacttccctg caaatagtcc tgggacatcc gatggcatta tacgccgtta agcaggcatt
    23041 gaatgttccg gtggcattat actggtgcaa aacaggtgcc cttgaagttg taagcaatgt
    23101 atccccgtca ccaaaatccc acttaaatct aactgcatcc aatgatgaaa gattattgaa
    23161 agttgttggt gtgtttggcg ctggtgaaac gggcgaaaaa ctaaaatcag gtgttggagc
    23221 atccaatacc tgtatggtaa acctcgctgt atcggataag ttacaggtgt tcgggttaga
    23281 tgcaattaaa acaatattat aggtaccggg tatagcatat gtatgcgtgg ggcttgcaac
    23341 agatgatgta ttgccatcac caaaatccca gaagaaagtc tgacctgcca acgaagtatt
    23401 ctcgaaaaca gcatcaaagg gtatacaacc tgtaggtggt acaataaact gagcctttac
    23461 attatctgca acatttaacg ggatgaccaa agaatcagga gcattacaat atccagtatc
    23521 ctgcaggatc aattttacat tataagttcc gggtgccaca tatgtatgga gtacggaagc
    23581 agggcctgct gtaacccttg gagaaccatc tccaaaatcc catataaagg attgaggccc
    23641 gaatggtctt gctggcggtg ctacagataa attatcaaac cggtaattaa aggatgcaca
    23701 gggtggtaat tttaccgggt taaatccgag agttgcttca aggtttccta ctcttatccg
    23761 tacataagat gtatctctta tattacatgt tgtcgggtca attgcaataa gcattacagt
    23821 aaatgtgcct acagtattgt aagtatgcga tgtggaaggt atagcagtgg tgacaaccgg
    23881 cgaaccatct ccaaaattcc attcatagct ttgcgcatta gcaattgtat cagtaaaatc
    23941 aactgttaaa ggcacacaac ctgccgtatc cctgggaaca ccattaatac ttgattgtag
    24001 cccggctcgt acccctgata ggtccatgtc tatttttagc atggtcaggt tacattcccc
    24061 acctgtgttt gcgttattta cagtagacca ggcgccggct gttacgggat agagtggtct
    24121 tggtggcgca cttaaattct tacaatttcc acatattgcc tgatatattt ttccattccg
    24181 gtcaaagcga cttgtacccc catcgacatg gtcgcatcct tctcccggcc gattattttc
    24241 accgaagaaa ctgccaaaaa gttgagaaac cccattcctt tccaaaacaa agaagtaaag
    24301 atccttacca tctgtaactg atttaaaagc attcggggta acaggcaacc cgtttgtccc
    24361 ggcactatta tagccgttat cagtaccaaa aaaacctccc caaccggata tatacacatt
    24421 ttcacaacga tcaactaaaa aagcaattgg cgatatatta ggcgtcaatc caccagagcc
    24481 aaaggctgtt gaatatacat atgctgaaag atcaggttga agcttggcaa taaattgttt
    24541 gccacccgga ttattataag gaggattggc taccggaagt atttcccagg taccagtggt
    24601 ttgccccatt atataaggaa acccattggc gtcaaactgc acaccaaata cctggtctat
    24661 tccatcagta ccgatataag ccgaacgaat aacattagta cctgaattat taataatagc
    24721 aagaaaccca tcaatagggc cacgaatgga aggacctatt gtacctgttt gtgaattggg
    24781 taataagtct ccgctctccg ttccacctgc aacatagata tttcccgttg tcggatgaag
    24841 tgataataca taagctgcat cgttagcaga acctccaata tagctggcaa aaagtaatgt
    24901 atttagatcc ggggatagtt ttaaaacaat tccatcctgt gttcccccac caaatgttgg
    24961 ttgaaagaaa ccggctgtac caggaaaatt attggactga gtgctggaag cgacatatat
    25021 atttccacta ccatccagga taacttcact gcggccatca tcgccataat ttctttgtag
    25081 tgaagtggca ctacccctgt tagaagtaat atttacacca tcattgccag taccgccaat
    25141 ttttcttgat cctattaagg ctgaaccggc agcgttaagt ttagtaacta caatatcata
    25201 accactgcct gctaatgcac cattattcaa caccgggtaa ttccctgaat ttgttcttcc
    25261 agccagaaca agatttccta aggggtcaac aaacaaacta tgtggttgat cttcgcctga
    25321 acctccgata taagttgcat agacacggtt tgatccgtcg ggagttaatt tgattattcc
    25381 aatatcaaaa ggaccaccct ggtaattggt ttgatatgct ccgggagatg tgggaaatcc
    25441 accaccaccg tcaaaaacaa tcccgccgcc aaacatactt tcatccgggc cataagtagc
    25501 agtaaagccc cagttatcag aggaactacc tgaaaaggaa cagaaaatga tttggggatc
    25561 aattacgaga gttgtggtgg gatcatagtc cttcacatca aatctcatca cattatcttt
    25621 tacaacatac ttacaggata tttcttttcg tccggttata cccgcttgaa atgtatatgg
    25681 agcagactct ttaaaatccg ctaatgaagt ggaaataacc agttctttat tttttacctg
    25741 caatttatcc accccctcat acttcagggc aattttagaa acatctccac cgggcttcac
    25801 caggatatca tatttaagag taccacggtc agtatagtat cggacatcta cattcgggta
    25861 aacatcttgt atagttattc cctggtaaat gcggcattca gatgcccatt tggaaggctc
    25921 attgccaata aaataattat tataagtagg aatgggtttt tcaggaatga gcttcaagtt
    25981 cttcgatgcg cctataaaat ctacattcca tgcatgagag cggataacca aactttcatt
    26041 tgcggccagt ggcttgccgc ttccattaag tccatgttta aattgagaca tcctggctac
    26101 atcagccgga ttatgttgca ccacggtaaa gccaccattg cggataaaaa aaactccatt
    26161 acttacatcc cctttatatt gcacacggct atcccattgg cctttattct caataaattc
    26221 aatatttccc tgcgctggaa cagtaagggc aggaaataaa aatacggcga gtataaaaag
    26281 atatttcagg gtcaaattat tttcattaaa ataaccaatt tttcccggaa aagggttgaa
    26341 aacttctgtt aacaattgtc ttgtcatagt aatattttcc tctacccgta aaatccgggc
    26401 ctgacagcct tttacatgtt ttaagatatc ttactccact gcactggtcc tttcttgtaa
    26461 tgaaggaact tctttagctg caaattttct gccgaaacca ggtccgggtc attttctaat
    26521 aattccagcg ctatattttt agcaatttcc aaaatagacc tgtcttggac tatactcgcc
    26581 agcctgaagt ttaaagcacc gctttgtctt gttccatcta tttctcccgg tcccctcaac
    26641 tcaaggtctt tttcagctat ttcaaatcca ttgttcgttg atgtcattat cctgatgcgc
    26701 tcacgggcat cattacccag tttggaacct gtcaataaaa tacaataact tttttctgcc
    26761 ccccggccta cccttcctct caactggtga agctgggata agccgaattt ttcagcactt
    26821 tcaatcacca tcactgaggc attaggcaca ttcaccccta cttcaataac agtggtggaa
    26881 accataatct gggtatctcc ttcaacaaat cttttcatat ttagttcttt ctgctcagca
    26941 ggttgtttgc catgcaccat gcttatccag tattttggtt cggggaaata ggcttttacg
    27001 ttttcatagc ccttcatcag gttttcatag tccatttttg aactttcttc aattaaaggg
    27061 aaaatgatat acacctgcct acctttttct atctcactcc ggataaaatc catcacttta
    27121 ctgcggtgag actcataacg gtgaacagta ttaatgggtt gccggccggg aggtaattca
    27181 tctatcacac tataatccag gtctccatag gctgtcatcg ccaatgttct gggtattggg
    27241 gttgccgtca ttaccaatac atgaggcggc acttctgcct tttgccataa ccgggcccgc
    27301 tgtgccacgc caaaacgatg ttgttcatca ataatgacaa taccgagttt tttaaactgt
    27361 accacatctt caattacggc atgggttcca atcaggattt gtaaataacc ctctgataaa
    27421 tcctgtaatg tttttttcct gcctgctgat tttgtagaac ccgtcaataa tttaacggtg
    27481 acgccaattt tttccagtaa taaaccgatt gtctggaaat gctgttgtgc caaaatttca
    27541 gtaggtgcca tcaggcaagc ctggtagccg ttatcaaccg ccagtaacat ggtaagcact
    27601 gccactatag ttttaccact gcccacatca ccctgcagca gccggttcat ttgcttacct
    27661 tttgctgtat ctgcccgaat ttcttttaat accctttttt gcgcccctgt caactcaaat
    27721 gggagataat tcctatacaa atcattaaaa tattccccca ccctttcaaa aataaccccc
    27781 tttgaaaaac ggtgccgctg gcttcgcaac aggttcatcc gcatttgggc aataaacaat
    27841 tcttcaaact tcaatcgttt tacagcgttc tcatactctg tatttgtttg tggaaaatgt
    27901 atttgcttgt acgcactgaa ccggttcact aattgcaatt gttctaatac agaagaagga
    27961 agattttctt ctatgtcttt ctcgcgtatc atccccatca ataattgagt tagcttaccg
    28021 atttgtctgc cccccaatcc tcttgctttt aatttttcag tagttgaata aaccggttca
    28081 agataatttt ttgccaattg ctgttccgga gataatatct ccaattcagg atgaacgatc
    28141 tgcggtttac cattgtaaaa actaaccctt ccataaacaa ggtatgactg cccggcctct
    28201 aataatttgg ccacccagct taatccctgg aaccaggtaa gctctaaaaa tccggtttta
    28261 tcttttagtt ccgcaactat ccggcggccg gctttttggc caacaacatc aaaatttact
    28321 aataccccag ccacctgtac aaattccgac tgaggcgtta tttcgctaat gagattgatt
    28381 ttcgtcctgt caatatgacg ataagggtaa tggtctaaga ggtcaccgaa agtaaatata
    28441 ttcaactcct ttttaagtaa gtcggcccgc tgggggccaa cacccttcag gtactcaatt
    28501 ggatttgata atatatgtga agaagtggaa ataatatatt gtttacagca aaaataacaa
    28561 atcgcaggac cgctgaggct attgattatt gacgatctgc ttaaatgtta ttatacatgt
    28621 cgtttagcat tacttactct caaaatctgt ttttcgttga aaaaaatcta ttcattcatt
    28681 tgtaaaacaa ctacccgcaa aaggaaagca gataggataa caaacccttc gactataact
    28741 tatcaatacc actaaatcct tcctgttaaa caaaaagaag ccttaaatga tcaggcagag
    28801 ttcaaactgc aattatttta tgtgaaaatt acgattagaa actagcaaat atccctgtat
    28861 aagagttaat atccgcagtt cctaaagcgg aaaaatattt ttttgacctg cacaatattt
    28921 ttccagttgc aaaaattaaa aatgctgctt aatttgaatc gtaaatccac taccagtaaa
    28981 aagccatcac taatgaaaaa ccacattccc cttttacagc tcgatctgta tttttccggc
    29041 tataaatttg ccctgctttg ttttataact gcgttcgtga taacacttat cgccatacct
    29101 ccaatcataa gcctgattaa aaaataccgt ttatacgatg ttcccaatgc ccgtaaagtg
    29161 catgccctac ccatacccac catgggcggt gttgccatag ttggcggcat gatgactgca
    29221 ttgatattat ggtttccctt tagcaatcaa ttggcacagg tcagcttttt cttttcagtg
    29281 gtggtactgt ttggtttggg tattatggat gaccttaaag atctttcagc aaaatataaa
    29341 tttgtcatac aactgggact tgcagcatta atagctctat caggtataag aataacttct
    29401 tttgaaggtt tatttggcat tgaagaactg ccattaactg cacagtacac ttttactatc
    29461 ctggctatcg tgggtatcac caatgctttt aatttaattg acggtattga tggtcttgca
    29521 ggtggattag gttttatgag cctggtaact ctcggtatat ttcttaccat gagtggcgat
    29581 gctaatactg ccctgatagc ttttgccctt gcgggaggca tacttgcatt tctttatttc
    29641 aatttcaatc ctgccaggat atttatgggt gataccggct ccttagtatt aggatttgta
    29701 atagctgtcc tatgtataag gttaatgcaa gtgagttcct taaccggtaa tccagtatta
    29761 ccgcatgccc ctgtatttgt acttggcatt gtacttatcc ccgtttttga cacccttcgt
    29821 gtatttatgg ttcgtatctg gaaaggcaat tcaccatttg tagcagacaa aacacatatt
    29881 caccatctgc tgacaaacca aggtttcagt catgcatttg cttccaaact catctgcttc
    29941 attcatggtt ttgttctgat tgaagtctac tggctcaggt tgttgaagca ggaaataatc
    30001 ctgttgatac tgacagtaat tatgctgctt gcaatagtat tgctgaaaaa tatggggtta
    30061 gtattcagga ggtatgagaa agttaatact ttattgaaag gtgatttaag ttaaagggag
    30121 ttattggact ttcattaccc taacccagtc aaccagcatt ttacttttac cattttttat
    30181 ttgctcaaaa gttgaagccg gtaatccgta gtatggctct ttgatgccat taattttaaa
    30241 tggataaaca ccgccgacag caaagtttaa aataagatac ttgggattat caaaagccca
    30301 ttctccaaaa aattgagtca taggttttgt tactctaaac attggaactc cgtcatattt
    30361 aaaaatcaga ctgtccggtg tccagtccac tgcatatata tgccagtttg taacatcatt
    30421 cccggacgga aagtataacc tgtcaaccaa accagcatca ccactatacc cttttccatg
    30481 gacggcagaa ctagcccagt ctttttcccc gacatactcc atcacgtcta tttctcctgt
    30541 ttccggccaa ttgccggtac caagtatcca ccaggcaggc cagagaccag atccttccgt
    30601 tagctttatc cgggccgatg cagaaccata tgtaaattct actttactcc tggtattaat
    30661 tctgcctgag ataaaatcaa atttttgact atccttagtg atatagccag gcgaataata
    30721 aggctgtaaa accaatgccc cattctccgc accctcagcc tcagcgccag ttaccaaacg
    30781 tatcacatta gtggagtcaa tatatgcctg caattcatta ttgacatgca taccagtcac
    30841 ctctgtattc cacttcgaac tatctagtac cgggcctgaa aaatcatcaa aaaaaatggt
    30901 ttgggtattt cccttttcat caacaggcga tactgttggc cctgaactac accgtaccaa
    30961 cattacagaa caaagtgtta caataacttg aaaacgcagg aggcctttca gataattcat
    31021 ataagtaaaa tttaaaaccg gtacaattat tcataaaccc ttacataatc aataaccaat
    31081 tgctgcggga aaatccgatc atcaacccca tactttccac cccagttacc tccgacagca
    31141 atattgagca ataaataaaa ttgctggtca aaaggccagg cggcacttcc gctatgctca
    31201 tttttaaacg tatgatactt ttcaccatca acaaaaaaag agatttgcgc tgctgtccat
    31261 tcaatagcat acacatgaaa agaatcacta aaatcttttc gcattgtgct gttaactttt
    31321 tgtgttccca acataccatt atatacttcc gtatgcacag ccccgaacag tgagtcgggc
    31381 aaatagccca cgttttccat gatgtcgatt tcaccactct ttggccatcc gccataaacc
    31441 caattggttg gcaacatcca gatagcaggc cacaagcctt ttccggccgg gagtttagcc
    31501 cttatctcaa aacgaccata tttccaatcc cctttatttt ttgtgactaa cctggctgat
    31561 gtataggatg caccggaaaa attttctttt cttgtttcaa taattaattt cccgttctct
    31621 actcttgcgt tctccggcct gttccatgta taaaactgta attcattatt tccccagcca
    31681 caaatatccg ggcaaccagt tcctttgtca aaaccccatt ttgcactatc gggcagaccc
    31741 ttgtattcaa attcatccga ccatacgagt ttggtaaata attcctggct gatacctgtt
    31801 ttctgtataa acatgaagca aagcatagta actatgtaga ttgtacttat tctcctgatt
    31861 ttcataattg atttttagat agtttccgga tagaaaaacc cagattaaaa tagtttgtat
    31921 aaaaagtgga ggtattaact taaaaaagta aatacgatcc acttactaac gactgcttat
    31981 gcttaaaact gcttgcttta aagagtaaga aattcagtat aaaattatat ctgttacctg
    32041 ccgaatccat ttgtaaatcg aaagcaactg tttaaataaa aacgctttta attttacttc
    32101 ttatatctta ttgatacacc cgtatataat caatttccat tgctgcactg gtaaagccgg
    32161 gatcaactgg tcctccgaaa gtaccaccca tggcaacatt caggattata aaaaaatttt
    32221 gattaaaagg cagcgtacta acattagcga acgtataaaa caccacgtca tccacagaga
    32281 atttaatagt tgtagctgac cattcaactg catacttatg aaattctgtt gtcacatttg
    32341 gaatagtaac tgtacctccg tctgcatttc cgcctgagtg gccgggatga tggacagtgc
    32401 cgtatatttt attcaattgg cttcctttat gctccataat atctatttcg ccacaggctg
    32461 gccatccaac agtgtttata ttattcccta acatccatat ggcaggccag gtaccaatac
    32521 ctgcaggaag ttttgccctt gcttcaactt ttccatattt aaatgaaaac ttatccttgg
    32581 acaatagcct ggctgatgtg taggcacttc cgctgaaatt ttctgcttta gctataattt
    32641 tcaatgtgcc atttgaaata attgcattat ctgttctgtt agtatagtat tgtaactctg
    32701 cattgcccca tccaccagca ccaagatcat aaccccacct ggccggatca ggagagcccg
    32761 gtgtatcaaa ctcgtcagac cataataatg ataacgctac tgttactgta acctggattg
    32821 attttgatat tgtttgcccg gaagcacttt ttgcaataac atttactgtg taggtgcctg
    32881 aagccggata acggtatgta actatcccgg atgccaccgt ttgaaaaaca ccattaccat
    32941 aatcgtagtc atatgtaaca gcattggttg ctgcagcagt aaaggaaaca ttgccactat
    33001 tatcagtact tacaattgca ttaacagtaa gattagtggg tgctacatta ttattaccgg
    33061 gagagtcacc ctttccacat gaggctacaa ttacagcaac tgaaaatatg aacaatggca
    33121 aatatttcat tgtattatgg tttacgtttt aatttctgat accaggaatt accatcaata
    33181 ccaatattcc tcaaatgaat actattatct gtaatagata atatctcata cgttgttcct
    33241 cctgtaccaa aatttataat tccgttaccg ggtaccataa attgaatcat tgttgaaact
    33301 gcactggtag aaccagatgt tgcatccata aatgcaaggg gccttgtacc gccggtattt
    33361 atagcatagc atccgtcacc accactaaag ccatagaaag cagtagaggc gccgattgaa
    33421 aaagattgac ctttattgtc tattgtcatg gataccctgt tattgacatc tttggaaaaa
    33481 gtgatctcat catcataggc acatggttct cttgtattag gacccgcggc ataccatatg
    33541 ggagaaaact ctgtagcagg accaacaccg aaatgcccgg gagcatcatt atcagttacc
    33601 catacttttg aagatccatt tgttagtgat tgcagaatac cagcaggaat ttcaaatgcc
    33661 acaaacactg ttattctttt actgatcgta gaaagcgatc ccccggttcc aactgcatta
    33721 acagtaatag tgtaagaata agtacccgga ttcgtgtact tgtaattaac tacccctgat
    33781 ggcacaaccc tggttattcc atccccaaaa tcaatattat aagtgagtgc atttgtagct
    33841 gtggtagtga tagttacaat acctgtccca ttaccattgg ggttagcagc atctacacct
    33901 gcaatgacag ttgtcagggt aagatccgca ggggtcttta tatccccgaa tgagtactta
    33961 tcttttttac agctactgaa cccaatcagt accgcaagaa aaataacagc gattgatttt
    34021 atattagtta tcataattat tactttttgc aattagttaa gttttatgtc atcaaacagg
    34081 aatgtgaaat tgggtgagcc atcgcccatt gttcccaatt caaagatcaa aacgatcttc
    34141 tgataggaat ttgcggtatt gatggcactg aaatcgaatg tcagctcttc ccatgcatta
    34201 gcggtagtag taaggacttc tttctcgtaa ctaatacccc cattggtttg gttttcaact
    34261 ttcaaaagca ctttggcacc tacccttggt gagaaaactt tcattttaaa agtccttagc
    34321 gtagaaaaat caattgggct agcgaggcca atccagctgc ctccccatac ctggccagca
    34381 ttctttatca tcctgccaac tttactactg gtattaatac ctgatgattg cggattattg
    34441 ataacagtcg catcgcctcc gtcaaaattg gtaaatgtat aaaccaatgt agttgattcg
    34501 aagtcaagcg gaagggcgat tccggcaaca ttcaccggta agaatgtaat atcatcccag
    34561 tagaagatct ttccgtcccc ggcagttcca aaattaaaaa agatggaagc catattatag
    34621 gtatatgcca ggttcaaagc agcagtcccg gtggcttggt tagcaaaatc aaaaaccagg
    34681 gtctcccacg cattggctac tgtggtaact ttttcagttt ctactgacct tgtattatca
    34741 ttgcgatcct ccactttcaa tcgtattggc agacccgctg cgggtgagta aacccttaca
    34801 ctcatttgag tggcagtagc tgaaaaagga atagcagatg taaaacctga tccaatggtt
    34861 gttccggccc atgtttccgc accattaggc tttgttgttt ttttaacgtt attcgcagca
    34921 ttggaaggat caacagcatc aactgattga tttccgccaa agtcagtaac tgcataatta
    34981 acaccgggaa gatcaaaagt tacaggcaag gttaacaaag aagttggaag ctggttggta
    35041 agccttatat catcaaaaag gaaagtaaaa ttagcagacc catctccggg tatacctaat
    35101 tcaaaaatca acaccacttt ttgataagat tcagccgtat tgatagcggc gtaattgaag
    35161 accaggtctt cccaggtatt cgccactgta gtagttgcct ctctttcaaa actaattcct
    35221 ccgttggtta aattctcaac tttcaacaac actttggcac caactcgtgg agaaaaaact
    35281 ttcatccgga aaactttgtt gacagaaaaa tcaatgggat ctcctaaagt gataacactt
    35341 ccaccccaaa cttccggagg gtttttcacc attctgccaa ccctggaact ggtattaatt
    35401 ccgctggatt gaggattgtt tattatggta acattaccgc cgccaaaatt tccaaacgta
    35461 taatcaacag taggtgattc aaaggtgata ggcaataata ccgggtctac aattgttacg
    35521 gacctggtaa cttctgcagt ggcagcaccg ccgctcaacg caactacacg tacggtataa
    35581 gttccgacag ctgcataggt gtgactaaca gtagcccctt ctaaaaatga tacaggagtt
    35641 tcgttgggaa catctccaaa gtatattctg aaatttgtct catacaaagc cgttgcagaa
    35701 acatttattt tatagttgtt agccggatca acattaataa ccacatccag gttttccgga
    35761 gccctgaagg aaacagttaa ttgcctggtg atttcagttg tctttccatt gatggaatag
    35821 gccacaattt taacattgta tacaccttcc gcataagtat ggattgtatt ctttccggcc
    35881 tgcacttttg caggggtagc cgtaccatcg ccataataaa tatcatagga aacagctccg
    35941 gcaccatttg gcgtaatcgt tacacggcca gtattatcct gggtgatctc aaaaagaacc
    36001 gataattgat ccggtgaatt agcagtatca acaaaggaaa catcatcgta ggtctccttc
    36061 ttgcaaccgg cagccagtac cagcagaaat gcaaaaccca aaaaatattt taatgatttc
    36121 atatttgtag ttttaaataa ttaatgatta gtatcctggg ttttgagcta gccctgaaat
    36181 atctatttcg gcctgaggaa ttgggaatac ttcatgttta ccggcaacga aaccagtaat
    36241 ctttgaagcc gcttgtccgg ttctcactaa atcgaagaag cgatctcctt ccatagcaag
    36301 ttccaacctc cgttcattca ggatggcctg gtacaaggca gcacctgtcg cactaatatc
    36361 atgaagatta tccccaaaag ccctgcggcg caccaggttc aaataggttt gcgcctttgt
    36421 atcatttgga gctgttgact tattgaatgc ttccgcggcc attaataaaa catctgcata
    36481 ccggatgatt ctgaaattat tcatgtaatt caactccacc tgtccggatg tttctccttt
    36541 tctggggagg tatttctggt tatacaaacc agtgttttta taaggtgcca cctggtaagc
    36601 aggcctaagt gcaaaagctg ggttatttga tgcaacaaga tttccaaaca gtgatgggtt
    36661 gttatttata taatcatcta tattaagtac tgttaccgct tttcttatat cgcctgctgt
    36721 atatgcatta gctaagttta cagtaggtaa attggtactc caaccagcag cataaggcat
    36781 cgcaccagtt ccattcaatc cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36901 nnnaaattgg tactccaacc agcagcataa ggcatcgcac cagttccatt caatcccctg
    36961 ataccacatt gttgaacagc aaggttaccc tgtcccctgg ttacccctcc ccagttataa
    37021 tagggagaag tattggaata ttgaatttca aaaacagatt caggaccgtt ttcaccggtc
    37081 gctaaaaata tagatccaaa atcagctaca aggctgaagg cattcgaagt gattacactt
    37141 tccaatgttg aagcagctaa atcaaattta ttttgataca ggtaaacctt tcccaacaaa
    37201 gcctgggctg catattttgt aattctgcct ttctgtactt gtacagctgg caatacagct
    37261 attgcattgg tcagatccag ctctatctgt ttgtaaatat ctgctttggg agttttatcc
    37321 aatgatttag attcacttaa gctcagtctt ctatccacaa acatcgggac atcaccaaaa
    37381 aatctgacca aatggaaata ataataagct cttaaaaaat aaacttcccc atataaagca
    37441 tctttaccgg caaaattcac tgtttcacca gccaggtttg ctgctttata ctggtgcata
    37501 taatttgccc tgttaatacc ttcatatgca gcttgccata actctgtaag tgtagagtta
    37561 acaggtgtat gtgtgtaatc atcaatttgc tgtaatgaca gcacatcaga agcattttca
    37621 ccaccgctaa ccgcattatc tgatgcaata tcgccaacag gtactgcctg gttgatccat
    37681 tgtataggcg tataaacact gatagccatt gtgcgataat cgctttcaga tttcaaataa
    37741 tcgagttctg ttatccgaaa atcttcatgc gggttataat ctacccactt tttacacgaa
    37801 gaaagcaatg tggtagcaat aaccacagcc attattttta ttgttatatt cttattcatt
    37861 ttgctggaat tttatagtaa agatttatat tttaatatca aggccaaaag aatatgtcct
    37921 ggcttgcggg taagcaccac ggtcgatacc agtatctaat attccgccag caatttccgg
    37981 atcatacccg gtgtacttgg ttaaggtgaa ggcattcttt acctgtgcat agattcgaag
    38041 cctgctaaaa atctttttag taactgatgc agggaaggtg taccccaact gaatattttt
    38101 aagcttgatg aaagaaccat cctcaacata cctgtcagac acccggatat tactattagc
    38161 atctgtaaat gaatagcggg ggtttctggc atcgtttgta gttccgggtc cggtccatct
    38221 tgccaatacg cccctgtatt tattggaaaa atttgcattt ctttcatatg cgctgtaaac
    38281 atcatttcct accgaagcat atgtaaaggc tgtaaaatca aaattcttaa attccatatt
    38341 caggttccag cccattgtga aatccgggaa tggatcacca atttttgttt gatctgatgc
    38401 attgatgata ccgtctttat tcatatctac aaaacggata tcaccgggct gtgcacctga
    38461 ttgtgccgga ccagcagcaa cttcatcagc ggtttgaaac aaaccatctg ttttataccc
    38521 ataaaaataa ccaggggtaa aacctttctc gaaaacagtt acactttgag attgaccgtt
    38581 aaaataataa ccacccagga ttttagctgt accatcatca ttggtggctg taacctcact
    38641 cttagcagta gtaaaagtta caaaagagtt taatttaaat gatttgccga tcgtctcatt
    38701 ataggaaagg ctaagatcta taccactact tttagtagaa ccaatattag cagtagggat
    38761 tggaacggtg cccaggtata atgaagccga aggggtaaac aataaaccat ttacatctct
    38821 ttgaaaataa tcggctgata atgaaaactt cctgttgaaa aaagtcatat caaaacccac
    38881 attggcctgc aattgctttt cccactgcaa cgcatcattg gcagcggaac caacagtaga
    38941 accaggataa aacacattat cgaatgtata tccattgctg ttaggtggat tgccataact
    39001 tggaccaccg gtaataattc ctacaaattg aggattgaca ttttcattgc cgatggagcc
    39061 atagctaccc ctgattttca aaaagtcaat aaaacttgag ttaaaaaagt cttcgctcga
    39121 tactacccat cctaaagatc cggaaaagaa gctagcgtat ttattatcct ttccaaatgc
    39181 gtaagatcca tccctgcggg ctgtgaagga ggctaaatat ttttcctgat aatcatagtt
    39241 tatacgggaa aaataagaaa ggtttctcct gaaatactgg taataatacc cggcaagtgc
    39301 attggtattg gtagcagaat tatttccggt agcagccgta aagtctgcaa attcccatga
    39361 attaaagggc acatcctgtc ttgttgcacc cgccgcatta ccagtcgttt ttgccagtga
    39421 aaagcctaat accgtttcaa aagtatgatc atcttttacg ttaaagttat aattcgcaaa
    39481 tgactcccag gtataattga aattgctggt tttttcatgt gccacgctat tatgcttacc
    39541 aaccactgca gaaccatcag cgttcattga attatccaca ttgttcgggc cgtagaaaat
    39601 aagcggcgtg aaactcttgg catttccatc atactttgta taaccaaaac gggaggagag
    39661 cttcaggttt ttcaacacat catactgcaa ttcaaacttt ccgtatagct tacttcctac
    39721 atttttatta taggtattct ctaatttagt tagcggatta aatatttctg atacgagaag
    39781 attgctgaaa ccatattgac caacggtatt gggaacggta ttatacttgg gaacggtggg
    39841 atcaaaattc aacgcacttc ccagaacact gttaaaggaa ttctcttgta ttccccttga
    39901 attgaggatg acaccggtcg tattaagtat taatttcaat tttggagcaa catcaaaaat
    39961 caggtttgca gttaaatttc cccttttgaa tttcgattta tcatctccac ctacaatacc
    40021 accctgatcc aggtaaccgc cagataaaaa ataagacatc ctgtcactac caccccgggc
    40081 tgtaatcgta tgagattgca gagaagcagt tttgaaaatc tctttctgcc aatctgtacc
    40141 cactcccaag acagacagat cagggaaaat aacatttccg cctgcgactg tactgccttc
    40201 attaataatg gcaccatact cagatgcatt taatacgcca atggtattaa tcacttcctg
    40261 tacaccataa ttgctaccta ttgttatttc tgttttttga ttttttctgc cagatttagt
    40321 tgtgacaaca ataacgccat tccccccctt tactccaaaa atagcagtag tagcagcatc
    40381 tttcaacaca ttgattgatt caatatcgga ggcattgatt gaattaaagt ctgttagtgt
    40441 ctggggaaca ccatctataa taacaaccgg gtcagtacca gaaaaagagg ggatacccct
    40501 gacaagaact gttggtttag accccggtga accactttga atgacattta cccctgaagc
    40561 ccttccctgc aaagcctctt cagttcttac gggccggagt ttttcaatat cggcactttt
    40621 aatggtcgaa atagcaccgg aaactttggt aattttttgc gtaccatagc ccactaccac
    40681 tacgtcggtc aaagttgtgg tggcactctc ctctaattga atggcaactg cagtggtatt
    40741 atctgccact ttatattcaa tcaccttcat gccggtgtaa ctaattacca gtactgagcc
    40801 tttttgggga acagtaatag agaaattacc cttttgatca gtaattgtgt tggcagatcc
    40861 accttttact aagacagtgg cccctaccag aggttctcct gttgttttgt ttgtaacatt
    40921 tccggaaact ggaagatttt gtcccataac ctggtggaga agaccaccaa caaggatgct
    40981 gagacttagc aatgcaagtt ttaatttcat aaagcaatag ttttagcgaa tcgaaaaata
    41041 tacgtaaaat aaggtaccca aggtactacc ccaaactctg tttaagaaaa aaacaaccac
    41101 atcaatacta catcaaaaaa tataacctat tgattttaag gcacaataca ctacttcatt
    41161 actacatcaa acgatttttc atactttcac aactgtaaac atttagtaca accatgagaa
    41221 aatttgctct tctttttctc tttctccctc aaattgcaac aggtcaaaat actatcggcc
    41281 tccctgatgt tatcaactac cccaaacaaa cctatggagc gggtctgcaa agctgggatt
    41341 ttaaacagga taaaaatggc atcatatata tagcaaataa tgaggggtta cttagcttcg
    41401 acggaaaata ttggaaactt taccctcttc ctaataaaac aatcgtaagg tcagttgaaa
    41461 ttgggccgga taataaaata tatgccggag gtcaggatga gatgggatat tttgccccgg
    41521 cagaaaatgg ccagttgcaa tacacttcac taaccgggct tcttggtgag aaagaaaggt
    41581 cttttggtga tgtatgggac attgcttcgt acaaaaaaga tattttcttt cgtactccca
    41641 atagaatttt taagctaacc aatgaaacaa tttctgtatt tatagccccc tctgaatgga
    41701 cttatttggg agcttgcaat gatgttttat ttgcacacga ttacaaaagc gggttgctga
    41761 gatttgaaaa taatgtttgg gtgccattac ccatatccaa taacctgcct gttaacgacc
    41821 ccgtgaccgc catactgcct attcataaag acagtagcat aattacaacc cttaaaaatg
    41881 gcttgtttgt actttcaaaa acagggttct cgaaagtaac ctctcccgca ataacactta
    41941 ttgagaaaga aagaatttac gccgctacgg ccctgaatgc tgattggttt gctttagcca
    42001 ccaataatgg cggagtgcat ataattgatg cgaaagggga gctcattcaa aatttcacaa
    42061 aaacagaggg gttacagaat aataatgtgc tcagtatttt tttggataaa caaagtaatc
    42121 tctggcttgg gctggataat ggaattgatt ttattgccta taacagtgcc ataaaacgca
    42181 taacacctta tgcacaggac ggatcgggtt atactgcact tatccataat aattacttgt
    42241 acaccggaac ttccggaggt ttatacagcg taccattaca acagatgaaa gatcttagct
    42301 ttagcaaagg aacattttcc actgtttcta acacaaccgg gcaaacatgg gggttgtcag
    42361 aaatcaatgg caggctttta ctgggccatc atgaaggtgc atttattatt aatgaaaatt
    42421 cagcgcagcc ccttttatcc aacggcgccg ggttttggaa ctttgtacca ctttcttccg
    42481 tatttccatc tgccaggacg gtggcaggta attacaaggg gcttaccttt ttaaattata
    42541 acaataactc attttcaatt ggcgaaaccg ttccggactt caatgaatca tccaggtttg
    42601 ttgcactaga taagtatgat aatatttggg tatcacaacc ctatcatggc ttatataaaa
    42661 tatcaattgc cggagcaggt aaatataatg tgaaaccata caccagtaag aacgggcttc
    42721 cctctaccct gaataatcat gtgtataaga tcaaaaacga ggtagtagtc gcaacagaaa
    42781 aaggagtctt tatctataat ccatcgaaag acagttttga accttcccca ttttatttaa
    42841 atctattggg tccacaaagc attcgctacc tgaaagaaga tacagaaggt aatatctggt
    42901 tcatacatga aaaaaatttg ggagttgtgg atatgtcggc ccaacaacct gcgataatcc
    42961 atctgccgga attgaataat aaaatgctta gtggctttga gtttatttat cctgtgaatg
    43021 ataacaacat ttttattggc ggggagagag gttttttcca catcaactat gaaaaatata
    43081 aaaaaaatat ccctgcatta caagcgcagg tcagggctgt gaaaatagtc aataaaaaag
    43141 acagcctgct gttcggtggc tactttgcca atgttgatga taaacaaata cagggtaaaa
    43201 atcaaatccc ccgcatcagt aataactgga agacaataat gttcgaattc tcctccccgc
    43261 tattcggcca gcaggcaaat cttgaataca cctataaatt gaaaggcttt gatgagaact
    43321 gggccgaatg gtctggtaaa acagaaaaag aatacactca tcttgctccg ggaacctata
    43381 cattcgaaat caaagtccgt aataatctcg gcaacgagtc cgaaccggtt ggctacacgt
    43441 ttaaagtcct ccctccctgg tatcaaacta cccttgccta tatcatctat cttttacttt
    43501 ttggcctggc tgtatattat ttatacaaat ggcagcgaaa aaaattcaac cagcaacagg
    43561 aaaaatatga ggaagaacag aaaagacttc agtatctcta tcaactggaa atcaataaag
    43621 cagaaaatga actggtagca ctccgaaacg aaaaattgca gagtgaaata gatttcaaga
    43681 actctgaact ggctactact gccatgcacc ttgtacaaaa aggggaatta ttagcaaaga
    43741 taaaaggtga attaaaccag ataatgaaag tgttagataa tgaaaaagca gtaattgaat
    43801 tgaaaaaaat gataaaggta ttaagtgaag atgataaaat ggataaagac tgggagcatt
    43861 ttgcacaaca ctttgataaa gtaaacagcg actttgtagt aagcttaaaa gagaagcacc
    43921 caaccatcac aggtaatgaa ttgaagttat gtgcttattt acgaatgaat ttatctacta
    43981 aagaaattgc acaactgatg aatatctcag tacggggagt tgaaatcagc cgttaccgtc
    44041 ttcggaaaaa acttcacatt ccctcagaaa aaagtctttt tgattacctg atcaacatac
    44101 agactaaacc ttaacaggta gttactttcc gatacagaat ttgctaaata tataatccaa
    44161 ctgatcctca ttagtgattt ctcctgtaat ctcgcccaga taataaagac aacggcgtat
    44221 atccagtgcc agcaggtcac cgggtaattt attatccagg ccttgtttta tatctgtcag
    44281 cgatcgggat acttctttca acgcatgata atgccttgca ttggtaatga tagtattctc
    44341 agtctgtacc ttcccctgca ataccgcatc taccatcttt tcctttaata ccggcgtatg
    44401 aagatttcct ttagcagaaa taaaaattac attatcgata cccccgaact tatccttcgc
    44461 tattgcttca cccaattcat caatcttatt ggcaaccagc agaaacttta aggctttctg
    44521 ctcagcctcc tgtttccatc tttcaatctc acccttatca ttattcacat caaataaata
    44581 gataacgata tctgcctctt tcatcttctc caggcttctt tctattccgg cattctcaat
    44641 tttatcattt gtttgttttc gtatgccggc agtatctatc aaacggaata atataccatc
    44701 aatatttatt atctcctcaa tggtatccct ggttgttccg ggaatatcac taacaatagc
    44761 acggttctca ttcagcaaac tattaagaag ggtagatttc ccggcattcg gtttgccaac
    44821 aatggctaca ctcactccat tttttataac attaccaagc tgaaaagaat tgactaatgt
    44881 atccgttgac tgctttaatt cattaataag ttcatagaat tgttttctgt ccgcaaactc
    44941 cacatcctcc tgcgaaaaat cgagctccaa ttctatcaat gcagagaatt tcagtagcct
    45001 ttccctgagt gctttcagat tttcagaaaa acctccccgt atagtatgaa gggcagcctt
    45061 ttgagaggcc gctgtattag acgatatcag atctgcaacc gcctcggcct gggtaaggtc
    45121 cagcttgcca tttaaaaaag cccgctgcgt aaactctccg gctttagcta acctggcccc
    45181 ctgttttata caggccccga ttatttgttg ctgtatataa ggtgacccat gacaacttat
    45241 ttccacaata ttttccccgg tataagattt tggactttta taaattgata caaccgtctc
    45301 atccagcaga tcaggtcctt ctttcagaaa ccctacatgt aatgtatgag agccctgctc
    45361 aagaagattt ttggaaggaa acaattcatt tacaatacta attgcattaa taccactcag
    45421 acgtataaca ccaatagccc caacccccgg tggtgtggct aatgcaacga tggtatcatc
    45481 ccaaccagat agtttattca tcaccattca aaagtatagt aagtaaatga acttgaaaat
    45541 tttgccctta tttgaatcca caaaaaaatc ccctctgtta ccgaggggat ttttttaaat
    45601 atttcaaaag ttgattattc agattctacc ctgaacgtta tcggttgttt acggtaagct
    45661 tttacctggc ggccattttg tacggcagga gtccatttag ggcctttttt aataaccctg
    45721 attgcttcct cttccatacc ataaccgtga ttggtcaaag ctcttacatc actgatatta
    45781 ccttctttat ccacaacaaa ctgaactaat gttgtgtaag ttccctctgg cgcaccgtta
    45841 tcagtagcta cctgaccgtt ggcatttctt tcaaggtatt ggcgccattt gctgtcacca
    45901 cccgggaaac tagcttcaat ttcaactttg tcgaagatct tgttttcatc ttcttctttc
    45961 ttaacctcaa ctatttgttt tccctggtca atttcaactg gtgcaacaat tccctggtcc
    46021 ttcacccctt ctacgttcac cacatcaatt ttagcttctt taagctcttc ttgtggtggt
    46081 ggtggctttt ccacttcctc gtccttttta atgacgggag gagtgaattg cttcatttcc
    46141 actttagggg gctcctgttt tggaggcggg ggtgggggcg gtggaggtgg ctctttcttt
    46201 tcctcctgct taatatcctg aatggtcatt tcattcattt ttaccttgct gtcaccctta
    46261 tcacttaaag tactggatag tactgacgct aaaagagcca gtaaggctac agatcctgtg
    46321 attacaaggg cacgggtaat acggcggttg taagtttttc gtaactcata agcaccgtag
    46381 tctttattct ttccatcaaa aatgatgtcc agaatatcgg ctgatagtat tttgttggct
    46441 tccattactt aacgttttta attagatgct gagaggatta tttttccata aaccaaacag
    46501 cttattttat cccactcgat gcttcagtcg ccttaatcag ttcaaattcc tggtcaaaaa
    46561 ttttcaccat ggcgtaagta cgcacctggt taattgtcat ttcgtccaga atatctaccg
    46621 tattcttata cgtagcttca gggctgggtt taataataac aacaaaatct ttgtcagcac
    46681 attcactgat tggtctaccc tttgccctgg ctttagcctc acattccggg tctgtaacat
    46741 aacgggacat tacttctttc tttttgttta ctattatatc ccgtattcct ttgaatgtag
    46801 tggatttaaa attacttcca tcctcagtta gtttcccttc gtagtaaaat atctggttat
    46861 ccttacccag cattatacta agtacagagg attcttttac ttcagtttgt tctttctgat
    46921 ccttaatatc ttttggcata ttaaggtcaa gcgtagtggg actgctcatt gttgcagtga
    46981 atacgaaaaa cgtaatcaac agaaagccca gatccaccat aggtgtcata tctaccctgg
    47041 tagaaagttt cttggccttt tttacacccg ggcctttttt gtgaccacca tcacccccac
    47101 cggtatctat acttgccata taaaaagttt tttaggttaa ctattttttg agatcttttt
    47161 ctccggattt ttcttttata tataattctg tacccggtgg tatatcatct aaggctgtaa
    47221 cgaggttata tttaaactcc tcattacgct tcagtgctgc aattaccgcc tcaaaagtgg
    47281 gatactttga tttaccatcc cccttgatca ggtaattcaa tttttctccc gcaaaagcat
    47341 tttttgcttc tcgtacccac caaaccaact gattattggc agaatcaagc acgggtatac
    47401 cgggttgctt taatttatcc tgttcttttg cattggttcc caacagttgt ttcaatccgg
    47461 caaaggggac acccaccaaa atagtttttt tgaaatttgc aatttcggcc gggcttaaac
    47521 ccagattctg gctggtatta attccattga taatagcatc gtatttacct gggtctttct
    47581 cagataaaac agaaaagaaa accctgttac ttgaatcaat ggaaatcata actgcattat
    47641 tctcaggtaa tttctgagat aaaacagaac ctggtgtggt aatttccacc ggttctggtg
    47701 gcttgaattt ggtagccatg atgaaaaagg aaaggatcag aaaggctatg tccacaaaag
    47761 gggtcatatc cgtatctgta ctcttccttg gtatcttaac acttggcatg attacaataa
    47821 tttaatattg agatgaataa gttgtcattt tccatataca aaatctgcac tcttatttat
    47881 agagcgaagc aaaactttgt gtcagggtga aacctgattc atcaataccg taagtaatag
    47941 agtcgatctt ggtagtgaaa atgttataca tgatcaaagc cagggcagat gtaccgatac
    48001 ccaatgctgt attatacagg gcctcagaaa taccaaccgc aagatctcct gcagcaccgc
    48061 caccaccttc ttcaccaagt gcagcgaatg atctgatcat acctaataca gtacctaaaa
    48121 gggcaataag ggtacccaca gaagtgatgg tagaaagaaa taccaggttc ttttgcagca
    48181 taggaagttc aagagccgtt gcttcttcca cttctttttg tatagcaatt accttttgat
    48241 ctgtgtttaa gtttccttca gtgatcattt ctttgtaacg gcgcaaacct gccttcatta
    48301 cattaccaac tgaaccacgc tgtttatcgc actcagaaag tgctgcatcc acattacggt
    48361 tagccaggtg atactgcact ttacggataa aatcagcaat agacccattg cctaaagcct
    48421 ttcggatagt aaggaaacgt tctatagaaa atacaattac catcagtagc attccaatca
    48481 gcaatggtac aatgataccc ccttcataaa ttcggtgaaa tgcatcttta ggtcctttgt
    48541 gatgcggcca aaatccacca gcagcatctg gctcactgaa tccacttggg tttcccatca
    48601 taaaacgcca gattgcataa ccggcaataa tacaaacaat cggagcgatc catgaaatgg
    48661 cattgctgct tttctttgcc tgaactgaag ttgccttaac tgaagctgtt gcagttggtt
    48721 taatctctgc catgatctta attttttagt tttttttaaa aagttagaat gcttttttcc
    48781 ttttcagagc tacaatgcta agaaaaaagt ctttaaaaaa atcgccttcc ttcatttttt
    48841 caggaagggc cttcaagtta aggatttttt gatacggaac aagtccctga taaataaatt
    48901 ggtgagaaat tcataataag tttagagaac tgaatgctgg tcaatcaaac agaataacta
    48961 ttcgtatcgg agggcttcaa tggggttgag gcgaccagcc tttaaagcgg ggtatagccc
    49021 tgccaaaaga cccacaagag tacatatgat gatcccatag agaacccagt cccatggaac
    49081 aataaatcct gttttaagta caatagaaaa catattaccc acgataacac ccagtacaat
    49141 accaaaaaca gctcccagaa tgctgataat tatggcctca aagagaaatt gacggctaac
    49201 catcttactc ttgcctccta ttgcttttat gagacctact tcccttgttc tctcagacac
    49261 cgaaaccagc attatattca tcaggccaat tgcggcacca atcagtgtta taagaccaat
    49321 gacagtggcg gaaacagtca ggaacctaag gctattcata gccttttcag caacactgtc
    49381 gcttctgtcc agtacaaaat tgttctcttc tgtaatattt aacttccgga tagccctgaa
    49441 tgatccctcg gcttccccca tcgcaacatt aacttttgta agctcatttg ccattactgc
    49501 aataacataa gaaaaaccag atggaaagtt tctttcgata ttgctatagg tcgttatgat
    49561 tacattatca cggctaaaac caaaagttga tccccggctt tccagcactc ccaaaacccg
    49621 gtacggaata ttattaatcc ggactaccga gttgacagca ccaccgacac cctgcttaaa
    49681 taatttatca gccacatcat accccaatac acaaacattc cttcctgtct gcacatccat
    49741 acggttcata tttcgtccgt actgaagtgc aaatccattt aaggccaaat aattttcatc
    49801 tccgccaaac atcataacat ttggacttgt cttatatgtt tcatgagaaa cgatattgtt
    49861 ccggttacca aaaatggata tgctttttac agagggaaaa tcaaaccgct taacaaagag
    49921 ttcggcttca tctttagtaa tatttttgcc aagacttgat gttttctctt ttcttttccc
    49981 ctttttggtc agttttactt cccgttggtt tccaccacca aagcgtatat ttctttcttt
    50041 aaaccggacg gtaaatgcat ttgcacccat cgtagaaaaa ctctcggaaa atttttggtt
    50101 catcgcttta atggcagtga taatgccaat caacgccatt ataccgaacg caattataga
    50161 tactgtaaga cccgtacgta atttgttact acgaatagtg cggaatgcca atgcaaatac
    50221 atcacgaaaa ttcatactgg agaagacaat gcgataaaaa aatcgcttgc ctttcaggca
    50281 aagatagtca atgatagcgg gtattatttt atcagaaata aagccagttc agaaggaggt
    50341 cgggaaaaag tcccagcaat attaccaggg aggccattag taccattgtc cacttgaaga
    50401 aaggggaaaa gccagtagta gcatcattac cctccttaaa atacatggcc tgtattaccc
    50461 ggaagtaata atagacactt acggcagcca tcaggacggc aaaaatgacg agccaaagac
    50521 tgtttggagt ggctatcgtt gccttcagca tatagaattt agccaggaat cccgccgtaa
    50581 gaggtatacc tgccagggaa agcagcagaa tagtaagtgt agcagcaatt aaaggttccc
    50641 ttcttgccaa gccattaaaa ccatcaaatg tataatcatt catctttgcc agaaccgcaa
    50701 aaatgccgat ggtggctaag caataggcag ctgcataaat caacaatccc tcttttgcct
    50761 ccgtattcat agcatataac gacagaagca tgaaccctgc ctgtgctata ctggaatatg
    50821 ccagcattct ttttacactt tgttgaaaaa cagcagtaat atttcctaca aaaagagtga
    50881 tggcggtaat aaccgcaatc cataaacgcc actgtggctg taacccttca aaagcatcgt
    50941 caaacaaccg aatgaaggca ataaaaaccg ctgctttcac tatagttgcc atgaacgaag
    51001 taaatactgt cggggcaccg tcatacacat caggtgtcca aaagtgaaag ggagctgctg
    51061 acactttaaa cgccattgag aacaataata acgacatccc tgtaattagc agtgtattag
    51121 gtgccgccgt caccaatgga tcaggcacag ttataaaagt acccctggct ccataaacca
    51181 atgcgatacc catcagcata agaccggtcg aaaacacccc caacaggaag tacttcaaag
    51241 acgcctcatt acttttgagg ttacgcttat cgcttccggt caaaatataa agcggtatag
    51301 aaataatttc gataccgaga aacaatatca ataatgattt aaaggatgag acaagtgtaa
    51361 ttccacaaag aatgaaaaaa atcagggcaa aataatctgc ataattaact cccacttttt
    51421 caatttcttt tgctgacagc agcaggtata ataaagtgga aacaaaaaca atggtattaa
    51481 aaagtaatgc aaattggtca aatgcaatca taccacggct atctattgta aagaagacgg
    51541 tgcctcccat ctcaagaata ttcgccagta acaaaattat tattccgccg atcgcaacat
    51601 tacgtacagc agttttctgt ttcagcagaa taccactgaa catcatgaca acacctaaaa
    51661 tagccgatat gattaatgcg ttcatactat ctgttcctta ttgcttgcgg aataaatgac
    51721 ttatatctat tttattgacc agctcctgcg aaaaattatc cgtaacatcc aacaaaacct
    51781 gcggatatac acccatccag aaaatcaacc ctactataat tcccagtgcc agtttttcat
    51841 ttagcttcaa atcagttgct acagctgtta attcatttac ctctccataa aaaattctgc
    51901 ggatcatatt caatgtatac acagctgcca ggataattcc gagtcctgct aatacagtaa
    51961 accacaggta ataatttgac ttggcattca tcagtccatt aaacatcagg aattctccgg
    52021 caaaagcgtt agtcaggggt aaggcaatat tggctaaagc aattatcaca aagaaaatag
    52081 ccattacggg tgccttttgt gcaagtcctc ccaattcaga catttttctt gttccaaaac
    52141 gtcgttcaat gatctctaca atgatccaca aaccgatgat attgatacca tgattaaaca
    52201 tttgcagcat gacgccctgc attccgcttt tgttttcagc aaagatggca gcacacatta
    52261 acccaatatg agcaattgat gaataggcca ctaaccgttt tatatcatcc tgcctgatgg
    52321 caattaacga agcatatata ataccagcta tggcgagaga cattacaaca tctccccaca
    52381 catatgaagc gatcggaaat acaggtagca gccaacgcag caaacccaac aatcccattt
    52441 ttaccataac tccacttaat atcattgtgg ttggtgtagg tgattgctca taagtatccg
    52501 gctgccaggt atgaaaggga aagaccggca ttttgattgc aaatgcaaga aagaatagcc
    52561 agaacaacca ggattgagct ttagaactta gttcaacaac ataaaatgca ttcaaactaa
    52621 aagaatggct taccgttttt gactggatat acaaaatgcc gatcagcatc agtacagaac
    52681 cgacaaatgt atagatgaaa aatttgaacg taaccggtat tcttttttct ccaccccatt
    52741 gagaactcag aaaatacatt ggtataagtg ccagctccca gaagaaataa aataccagcg
    52801 catcggttgc cagaaaaact cccatcaaac cagcctgggc cagtaacatt aaggcaaaga
    52861 aattatttgg ttttttataa gaggcattcc agctgcccaa aaacaccagg ggataggaaa
    52921 tcgctgttaa aaggcagagc aactgtccca tgccatcgag ctttaaagag aaactgctgc
    52981 caatcggttg catccattgg gctgtgaact gaagattttc ctctgcattc atcagcgtta
    53041 aacctaatac ggaaacagcg agtgttgcaa gcgaagaaag gaatgcaaaa ctctttacca
    53101 ctttttcttc ttttaaggaa aaagcgataa gaccagttag cagtggaaga attattaaga
    53161 aaaatgcaac catatcatta tcccgcaaca agcggcttat ttattttcta agaaaaaatt
    53221 gtaaggcaaa aattattacc atgctcaata ccatcaacaa tatataacta cccacttgtc
    53281 cgctttgcaa caaccgtaac tggcggcctc cataattcac tgctcttcct accccattga
    53341 ccaacccatc aatcaccgat ttctcaatga ccgatttcag aaagccccca aactggtgca
    53401 ttggctgaac aatgaccttg tcatataact catccacata ccatttattc tcgagtactt
    53461 tacccaggcc tttttcttct gtccgctgat aattcctgaa cttaaaccag gcggcgatta
    53521 tcattattat caccaaagct gttgataatc ccatcagcat atattcagtt gaatgggaaa
    53581 cggtgtgccc tccatgcggc ttgataacag gagacaaaaa agctccaatt ctatctcccc
    53641 catttataaa gacttcaggt attccaacaa atcccccgat gattgaaaga actgctaata
    53701 caatcaatgg aatggtaata gccgctgggc tttcatgtaa atgttgtttt tgatcagctg
    53761 ttcctctgaa ttgccctgaa aaagtaagaa acaataaacg gaacatataa aaagccgtaa
    53821 gtaaagctgt aaataaagca aggccgtaaa gaaccgggct tttctcaaat gcacttaaca
    53881 atatagcatc tttagagaag aatccgctga atggaggtat acccgcaata gcaatacaac
    53941 caataaggaa tgttacaaaa gtgattttca tttttgtact caatcctccc atattccgta
    54001 tatcctgttc accgctcatg gcatgaatta cactaccact tccaaggaat aataaagcct
    54061 tgaaaaaggc atgcgtcatt acatgaaata ctgctgccac ataagccccg gtacccaatg
    54121 cgaggaacat aaatcctaat tggctgacag tggaataggc caatactttc tttatatcat
    54181 tttgtttaag cgcaattgtt gcagcaagta atgccgtagc caaaccaaca tatgccacaa
    54241 tatccatggt aacggaggac atggaataca atacattact tcttgcaatc atataaatac
    54301 cagctgtcac catggtagct gcatggataa gggctgaaac tggtgtaggg ccagccattg
    54361 catccggcaa ccaggtatac aggggaatct gtgcactttt accggttgcg ccgataaata
    54421 ataaaagtgt gattgccgtt atatctgttc cggtgaattt tctcaggtcc acttcaccca
    54481 tggcaactgc aaatacatca ttgaaattaa cattgccaac cctgctgatc atccagaaaa
    54541 tagcaatcag aaatgcgagg tcaccaatac ggttcataat aaaagccttg ttggcagctt
    54601 tattatattc tgtattctta aaccagtaac caatcaataa ataagaacaa agacctaccc
    54661 cttcccatcc tataaacatg atcacataat tggctcccat caccagtaaa agcatagaga
    54721 agacaaacag attcagatag gaaaaatatc tcccgaaatg ctcctgtttt tctttgtgca
    54781 tataggaagt ggaatacaca tgaatcagaa acccaacacc agttattatt aacaggaata
    54841 aagatgaaag ctgatcgacc tggaacgcaa aaggaatttt cagatcaccc actttgataa
    54901 aatcgaaata atgagctaca tagggagtag tcattccttt tacctgcata aagacccaaa
    54961 tactcatggc aaaagatgcg aggatgacac cagagcctat cacacttacc agggattttg
    55021 acaagtgttt tctgcccagt ccattgatca aaaagccaat caatggaagc agcggaatca
    55081 ggtaaactat ttgtaaagca ttttgcataa cctatcttct gaattccagt atcagtgttt
    55141 taacctgttt aaaaaattga cgtccaccga atggatattt cgatacatca ttacaatgat
    55201 cgctaatccc acagttactt cagctgcagc cactaccatg ataaagaata caaacaattg
    55261 cccgtctgtt cctgccgtac cattaacatc acctgcgagt aaagccgctt tataatgcat
    55321 ttttgaaaat gcaaccagca tcaggttaac cgcattcagc attaactcta cgcacatgaa
    55381 aattataata gcattacggc gggtcaaaac acccacgata ccgatactga acagggctat
    55441 agacaggaat atgtaataat taatgggcat gtcattcttt ttttccaatt accaccgcac
    55501 ctaccatggc acttaaaaat aaaatactgc ttatctcaaa aggcactaca taagtagtga
    55561 acaattcttt tcccaggttt ttaatcaggc caatagtccc ttcatttacc attgcttgct
    55621 ggttcttaat atcagtatcc tttaatgcag caaccataac caataataaa catcctcccg
    55681 ctactgcacc tgccattttt aaccagcggt gcttttgtgg ctctgtttca ttattcaggt
    55741 tcataagcat tattacaaac aggaataata ccatgatagc ccctgcatat acaataatat
    55801 tcactattgc caggaactgt gcattcagta aaatataatg tccggaaata gaaaagaaag
    55861 ttacaatcaa ccaaagcaca ctatacacag ggttcttact agtaatcacc atcaatgcgc
    55921 tgaagatggc cactacagac aaaaaccaga ataatatttg agtaatgttc atttctttta
    55981 agccgttagt cgttcttagt tctttaccgc tttctcacct aatgctttct cgtatgcctc
    56041 tttatctttt attggatgag gtatcagcaa ttcttccttt ttataaataa atccttttct
    56101 tccatagtta gcgggggcaa aagtctgcga caagtaaatc gcatctttcg gacaggcttc
    56161 ttcacacaaa ccacagaaaa tgcaacgcaa catgtttatt tcatacttag ctgcatattt
    56221 ctcttcccgg tataaatgtt cttcgccggg taatctttct gccgcctcca tggtgatagc
    56281 ttccgccggg caagccactg cacataaacc acaggcggta caattctccc tgccctccgc
    56341 atcacggttc agcacatgta accccctgaa cactttggag aatgtccttt tttgttcagg
    56401 atactgtata gttgccttct ttttaaacag gtgactgata gtaatcccca tgcctttgac
    56461 aatattccag agataaatac tctccaggaa tgtcatcggt ttacggtcta cctgttttgc
    56521 tcgttgtgtt aacatacttt ataaaagttc gcaaatatat tgttatcagt ccgaaagtct
    56581 ggtgaccgaa agtcggaaag aatgtttctt tatcatcgac ttgtcatgct gacttcagtc
    56641 tatttattca accacaacat cactgccgcc gaaataatca tattcaacaa agccaatggt
    56701 atcagtttct tccatcccag gttcattaat tgatcgtaac ggaaccgggg aatggtccat
    56761 cttatccaga taaacacaaa aaggaagaaa gctattttcg ccatcaacac actaaaactt
    56821 attaatgaca acagccaggg ttccagtccc caggcagcct cattcacaaa tggaatatca
    56881 taaccaccaa aaaacaatgt agccatcact gcactgctga taaacatatt aatatattca
    56941 gcaaacaggt aaaagcccag tttcatagat gagtattcct ggtgataacc aaaattaagt
    57001 tcattttctg cttctggcag gtcgaacgga gtccggttac actctgccaa ggcacaaaca
    57061 aagaaaataa taaaccccaa tggctgataa attacatgcc atccattagc ggcctggttg
    57121 gccacaatct cactcatttt caaagaacct gtcataaaca aaacagcaat caatgccaaa
    57181 cccatgggta attcgtagga taccatttga gacgcacccc ggattgcagc tatcaatgaa
    57241 aatttattat tggatgccca gccgccgatc ataatcccat aaacacccac actcaccaca
    57301 ctgaatacga tcagtattcc catatctaca tcagcaacct gaagcggaat aattccttta
    57361 tcagtaacaa tcggtgctcc ccatgggatg acggtgcttg taaccaatgc ggttaccata
    57421 gccaatcccg gacccagaat aaacagtatc ctgttagaag ccagtggaat aatttcctct
    57481 ttaaaaaaga gttttcctcc atccgccaaa ggctgaaaaa taccgaatgg ccctgcccga
    57541 ttaggcccat gcctgtcctg cagccaaccc gccacctttc tttctgcata agtggtatac
    57601 atagcaataa agaaagacag cccgatgata caagctatca gtattgcttt ttctaaaatg
    57661 aaccacaggt caattgccag taagtacatg gtcaggtatt attgttcttt cttttcaaat
    57721 gttttactcg ttgccggccc tggtaaagca ctaatgtcaa cactgttcac ctcactcaca
    57781 tcatgaatat ccatcaataa ctttggctcc ctcccactca tcacatcagg cattgtttcc
    57841 tttggtattt gcaaaccaag gtaatgtccc tgcgaaataa cagaatgcct gttgatctta
    57901 gtcggtccct caatcaccca gtcgtttata tgtttcttat caaagcggca ggtattacat
    57961 atccatcccg gtttaccatc ataactctgc acttcacccc attcatcttt cctggctgta
    58021 actcgcaaca cttcatctcc acgttgccat agcgttactt taccacagca cttgtcgcag
    58081 tcacggtgtg catccacagg cttggtaaac cacactcggt tcttaaaacg gaatgtttta
    58141 tctgtcaatg ccccaaccgg acaaacatcg atcatgtttc cgctgaagtc attatcaatg
    58201 gccttagaaa tataagtgga gatttcggca tggtcacctc tatctagtat accatggacc
    58261 ctgttatctg ttaactggtc ggctacataa gtacaacgat aacaaagaat gcatcttgtc
    58321 atatgcagtt gtatgtaagg acctatatct tctttctcaa atattctctt cttaaattca
    58381 aaacgggtac cttcttttcc atgttcatag ctcaggtcct gcaaatggca ttctcccgcc
    58441 tggtcacaaa tcgggcaatc taacgggtga tttaataaaa gaaattccac cacaccagct
    58501 cttgcgtcct gcactctttc gcttgtaata ttttttacca ccatcccatc cattacatta
    58561 gtgcggcagc ttgccaccaa ctttggcatt ggtctgggat cggcagcaga acctgcagcc
    58621 acttctacga gacaggttcg gcatttaccg ccactgcctt tcaacttgct gtaatagcac
    58681 atggcaggcg gggtcacatc accacctatc tgcctggcag catgcaaaat ggttgtgccc
    58741 ggtgccactt cgatagtgat attatcgatg gtaactttaa aattcgcagg tgcttctttt
    58801 tttacttcgt cagccatatt aaaaattcat tcattcctat tattatgcag ttgccggaat
    58861 ataaatcggg tccgcataat gcgccaatcc aaaattcctt ctttgtgcat catccggatt
    58921 tgttatatgc cattcaaatt catcgcggaa atgcctgatc gccgcggcaa ccggccatgc
    58981 ggctgcatct cccagggggc aaatcgtatt cccttcaatc tttcgctgaa tgtcccatag
    59041 caaatctata tcactcatct tgcccttgcc cttctcaata ttcagtaata ttttttccat
    59101 ccagccggtt ccttcacgac aaggagaaca ctgaccgcaa ctttcatggc gataaaatct
    59161 tgccagggta taagtatgtt ttaccacaca ttgactatca tccaaaacaa tgaatccgcc
    59221 agaacccatc atggagcctg ttgcaaatcc accatcggcg aggctctcat agttcatgat
    59281 tcttttctca cccttcgctg tcgttaataa gagatttgcc ggtaaaatag gaacagatga
    59341 accgcccggg atacaggctt ttagttttct tccgttggca atgccgccac aatattcatc
    59401 actgtaaata aattcttcaa cagaaatggt catatcgatc tcgtaaaccc ctggctttct
    59461 gatattcccg caggcggaaa ttagttttgt acctgtagat ttcccgatac ctatcttagc
    59521 atattcttca ccgccttcat tcacaatcgg aacaacagca gctatagttt ccacattgtt
    59581 taccacagtc gggcaatccc ataaaccttt taccgcagga aagggtggct tgatcctcgg
    59641 attgcctctt ttaccttcta atgattcgag caatgctgtt tcttcaccac agatataagc
    59701 accggcgccc cgttgtatat aaatttcaca atcaaaacca ctgcccaata tatttttacc
    59761 aagccagccg ttattttttg cctctgcaat tgcctgttcc agtatgtcaa ctatccatgc
    59821 atattcacca cggatataga tataggaacg attggaacct aatgcatatg aggatgtgat
    59881 cattccctcg ataagcagat gaggaatgaa ctccatcaaa tacctgtcct taaatgtacc
    59941 gggctcagat tcatctgcat tcaccaccaa ataacggggc actccctcag gttttgccag
    60001 gaaactccac ttcatacccg ttgggaaacc agcacctcca cggccacgga gtccgctttt
    60061 ctttacttcc tcagtaactg catcaggtgt cattgtcttc aatgcctttt ctacactacg
    60121 ataacctcct tcacgacggt atacatcata gagcctgatg ttttcaatgt gagctttgtc
    60181 taataataat tttcttccca tgcactatcc ccaaagggag tttatattta atgtcttcaa
    60241 ttatcctttt acagcctgcc tcgcaaataa taaccatctc tttcctttcc ttacccacac
    60301 ttccagcact tttaaatgaa agttgccttc tttcccgttc aatgtggccg ctatgtctgc
    60361 tacaaatctt gcatgtgcca catttccatt tatcacaacc tgtacactgt cttctttgta
    60421 actgttgtag ttcatataac ctgtttccag gtttttaata aaatcagttt ttgtttccac
    60481 ccagccgtta ctatgtccgt aactcagcca cttatctgtt tgctgattga tggaaactgt
    60541 attcttattt accaatgcct gatgaaattc ttttaatgtt aaagtcaact tctcttcaac
    60601 ggtctgagca ttcgtaaaaa aaccattcag taaaagaact atgaataata actttttcaa
    60661 cccttagttt tttgccgctg cagcatttct gcattcagcg ataattgcat caattttttc
    60721 tttggtcagc tgttctttat aatattttcc ctgctgcatc atcggggcat agccacaggc
    60781 accaagacac tccaccgtct tcagggtaaa cataccatca gccgttgttt ctccgggttt
    60841 gatacccaat ttttcgtcaa tatattttac aatatcatca ctgccattca acatacaagg
    60901 gcccgtctgg caaacttcaa acatatattt accaactggc ttcaaattga acatactgta
    60961 aaaagtagcc acttcataca cttcgatagc cttcaggctt aagacggatg ctacataatc
    61021 cattgtctcg gtactgagcc agccaccaaa ctcctgttgc gccaggtgta ataccgggat
    61081 caccgcactc ttctgtttac cctgcgggta acgttcgata atgcgctgca cttcttttaa
    61141 tttctcatct gaaaatctca aattcatttt ctttattcat ttggtcatgc ttcggcaggc
    61201 tcagcatgac atataaactt tttatgcatc caattctccg gcaatcaggt tcaaactact
    61261 catcgtcatg atcgcatcac tcagcatgga acccttgatc agttcctcgt atgcctggta
    61321 atacacaaaa cagggtctgc ggaaatgtaa acgatatggt gtacgacctc catcactgat
    61381 caaatagaaa cccagttcac cattacctgc ttcaattgag ttgtacactt caccggcggg
    61441 gatctctgtt tcacccatga tgatcttaaa atgccagatc aatgcttcca ttttagtgta
    61501 cacatccgcc ttgggtggca ggtaatattc aggaacatcg gcatgataca cctccgcttc
    61561 ggcacctttg agatcctgca ctttccggta agcctgttct ataatactca ggctctccca
    61621 catttctttt tcccgaacca gccagcggtc ataattgtcg ccggttgtac caacaggtat
    61681 attaaattga aaatcttcat agctgctgta aggtgcatga acccttacat cataatcaac
    61741 accggctgca cggaggttag ggcctgtaaa cccataattc aatgcccttt ctgcactgat
    61801 agcgccggta cccatcgttc tttccatgaa aatgcggttt cgctggaaaa ggttttcaaa
    61861 ctctttcagc actttgggat attcccgcag gaatttttcc agtttttgga aggcgatatc
    61921 gttaaaattt ctttcaaaac caccgatacg gccaatatta gttgtcagcc ttgctccgca
    61981 gacttcttca tatatttcat agatcagttc ccggtattgc atcacatata agaacccggt
    62041 gtaagcacct gcatccactc ccacaattga attacagata aggtgatcag aaattctggc
    62101 taattccatg atgatgacac ggagatagtc aactctcttt ggtgttttta cacccagcaa
    62161 tttttcgcag gtcaggtgcc aacccatatt gttgaggggc gaagaacaat aattcaagcg
    62221 gtcggtcaat gtggtgattt gtgccagcgg acgacgttca gccagttttt caaatgcacg
    62281 gtgtatatac ccgatggttt gttctgtaga aacaattcgt tcaccatcca gttccaggat
    62341 attctgcatc acaccatgtg tagccgggtg tgtaggaccc aggttcagcg tagtggtggt
    62401 tttctcaata cttccttccg gtaatttgat atgatgttct gacattatta attcaatttg
    62461 ataattcgat aatttttcaa gtgggcaatt tagaaacttt aaaaagtcag aacccaattg
    62521 tcaaattgac gaattattac attgtctgat tatcctcttc caaacatttc atcatcctta
    62581 tcaattcttg tctggtcttc caaaggaaac tctttcctca atgggaaata atccatttct
    62641 tctacattca atattctttt caggtttgga tgccctacga aattgattcc aaaaaaatca
    62701 taggtctccc gttccatcca gttagccgca gaaaataatc ctgtggcggt ataaatatcc
    62761 ggttgactga tatcagtaaa cactttgaat cggatacgga tattgtctgt catattatgc
    62821 agatgataca ccaccgccag ttctcttcct tttttatcag gataatgtac agcggtcaga
    62881 tccgtcagaa aacgaaactt caattcctca tcatcgtaca aaaagttgag cactttcaga
    62941 ttcagttcct tcggggcttc gaaggtgagc ataccataag gctcttcaac atgggaaagt
    63001 tgatctccaa atttttcggt taatcttgct tttatcgttt cgttcgttaa cgccattgtc
    63061 gaattatcta atttctaaat tggctcattg tttctattgt ataccgtagc tttccaataa
    63121 tgccttgtaa tgctccccgt ttcttcttct caaactttcc ttaccaacta aatcctgaat
    63181 tctaagtacc ccatcaataa tagcttcagg cctgggtggg caacccggta catatacatc
    63241 caccggcacc acctggtcaa tcccttgtaa cacactgtac gtatcaaata tgcccccgct
    63301 tgaggcacag gctccaacag ccatcaccca tcttggttca gccatttgga ggtacacctg
    63361 ctttaccaca ggccccattt ttttagctat cgtacccatc accatcagca aatcacactg
    63421 acggggagaa aagccaactc tctcggcacc aaatcttcct aaatcataat gagaacccat
    63481 cgtggccata aactcaattc cgcaacagga agtggcaaat ggaagtggcc agatagaatt
    63541 tttcctggcc aggccaataa ctttatccag tgaagtggca aaaaaacctt cccccatact
    63601 gccatcaggc atttcaacta agctgatatc accttcaagg ggttttttga taagattata
    63661 tgatacagga cgagccatag taataatttg aaaaagctgt aattgaaaag tatcccttta
    63721 taacaccatt tcaaccgcaa agttccattc tcacagcatc aaattttcaa gttgctttat
    63781 taatcctccc attttaaggc acctttctta ataatatata taaacccgct aaggaagaat
    63841 ccaacaaaca tcagtaccgc ccaaaagcca tcccagccta atcctttaaa gttaaccgca
    63901 tagggataga aaaagatcac ctccacatcg aataacacaa ataaaatggc tgtcaaaaaa
    63961 tatttgatgg ccattggctg gcgggcatcc ccatggcttt caataccact ggtaaaaatc
    64021 tgcaacttat cagacgtttt tctttttggc cccaacaggt gggtcaccac catcaaaaag
    64081 gcaacaagcc caaccgcaaa agctatttgt atggccaccg gcaagtacga ggcggctgta
    64141 gcagtattcg atcctgtttg aagtaaatga atcataagct tttactctgg gcgcaaaaat
    64201 aaggcggcaa cctgaaaata ccttaactaa aaatcataag ggggtatgta tttggtcaaa
    64261 acctttcaaa ataaaaagcc cccgctaaaa tcagcaaggg acttttgtaa aagaagaaaa
    64321 ccaagtttta agttcttaca actatatttt ttgacgtaac tattttcgct gatggtttcg
    64381 gagtagctgg ttttcctgat gccggccctc ccttagatgg cggattagcc ggtttattac
    64441 ctgaagcttt ttccagaatg cttatatttt gctggatagc tgtatttgca ggatctaaag
    64501 ccagcatttt acggagatac tccaatgctt taattttatc ctttgcaata ttagcatggt
    64561 attgtgccag gtaaccagca acacccataa tataattctt attttttgct gtgtcttttt
    64621 ccaggatagc aaggtatttt tcagcccatg gaactgccgc tccattttcc atagtactat
    64681 ccattgccct gtgtatgtta tattccatca tatacccata cgtttcatct ggaaattttt
    64741 caacataaac atcaaaaatg ctgtcagctt tctcataatt ttttccctgg taataggcaa
    64801 agcctgcatc aaaataatcc cgctgccccg gattttcttt catttttaaa ataaccgtac
    64861 gcagatctcc ttccttaata cggccaatac tgtcacctct tgctttaaac ctgtcagcgc
    64921 ccagtttcaa gaggtcaatt ttcagggaaa gggtagtatc aatgccagca ccctgcaaat
    64981 aggtattata gatttcatct acagtcccgc ctgttttatc caggatatct gcccggagct
    65041 gaaaatcata aagaataata tcatccgggt tctttttggc aaaaaaatca tcactgttct
    65101 ttttggcacc tgcaaaatca cccttttgaa aataggcatc ggaaagcagt ctgtaaacct
    65161 tgggttttgt cttgctgcct aaagaactca ctacactttc ggcccgggtg gttgcgcaag
    65221 tataatcttt ttgcgcccag caaagttgtg catagaggta ctcatcctga atatcttttt
    65281 caggcaattt gctgtcaata tatttttgta attgtgtttc agctgcagca taatcaaggc
    65341 gataaaaata ataatagaat aaatcatagt aggctgctgt ataattagca tcttttgtta
    65401 tagcctcatt taaatattgt aataccagtt cccagttttt ttgagattcg aataattttg
    65461 ccaatcgcaa acttgccaaa ggaaaattag agttgatctc caatgccttt ttataactcc
    65521 tgaaagcttc cccaccacct tctcccggtc ttgcttttct gtaagcatta cctaacagga
    65581 caaatgtttc agcatttttc tctcctttat cagcagcagc cgataatttt tcaatagcat
    65641 aattaaaatc accgcccttg gcatcagtat tagcacgacc cactgcattc aatatctcct
    65701 ggttatcacc tttctttgtt cttgtcatgg tgagagccgt ctcaaacttc tgacgggcat
    65761 cactgccttt attttccagt aattcaacat gaccaagccc caccataata agtggcgcac
    65821 tgttagtaga ctgcattgcc ttttcatata actggcgggt gctggcgata cgggctgcca
    65881 tgatctcctc tgactctagg tatgtctgcc ccagccagta aattgcttca atattattgg
    65941 gatttgcggc cagcaacttt tcaaaggtgg taatagcact attatatcgt tgggcataaa
    66001 ggtgattttt cccttcctgc accgtttgtg ctttcagtcc gcttattaat aataaggcgg
    66061 gtactaaaaa tgtgattgtg gctttcttca ttttactgtt cacttttaaa agttgtaaat
    66121 ataacttatt tgtctactcg ttcagcttca ccggcctgat gcttagcttt ttttgtgctg
    66181 gcgccaggta agctcttctg aatattatct gtccgatctc tccgctcata aagtttgcaa
    66241 aacctgttcc cagtcctttg tgtttttctt tcagtatata aaccaaatcc cgtaccatag
    66301 ggtaccgttt caggtaaatg tttgcctggt aagcctttac aaacgaacct ttaacatccg
    66361 tgctttctaa atgagctact cttacttttt tcaaaaaact cagttgcgag gaatcttccg
    66421 ggttacctat ccaccccact cctataaaac ctatgacccc tggattcttt gcaacgtaat
    66481 caacaaccgc atcactggtc ctggctgcca tcgcattggg ggtaagtgaa tccccacgca
    66541 atactgaatc gatgataaaa cgtaccgtac tggttgcttt tacgccgtca aaaaccggaa
    66601 ttaaattttt cttaaatctt ccggtaagga tatctctgat gtccttcatt gtaaaaatgg
    66661 tatccggaga gtctggatga acaattaccg aaattccatc tcttgcaatg atcatgcttt
    66721 tagcatccac ccttaacgaa tccgataaaa aatttttttc ttcctctgag gctctgcggg
    66781 taatgatcaa catacgtaca ctatcgacaa ataaatcttt caaacattcc gcttcgggtt
    66841 tataatctac caaaatactg gtaccgggat gattagactc ataaacctgc acctgctcat
    66901 cgattaccgg tttaaaagtt tcatcagcac ttactcttat agttccccgg ttacggctgt
    66961 ccggtaattc attttcctgc tcttcatatg atttacatgc tgttaataaa aacaataaca
    67021 ggaaaacagg aaaaa
//
LOCUS       DAORTI010000005        63366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_219_length_63366_cov_6.556317, whole genome shotgun sequence.
ACCESSION   DAORTI010000005 DAORTI010000000
VERSION     DAORTI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 63366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63366
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_63366_cov_6.556317"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLO70_01500"
     CDS             complement(<1..998)
                     /locus_tag="PLO70_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ73158.1"
                     /translation="MDQKPATIKEIAQRLNVSVSTVSRALHDHPSIGLRTKMQVQKLA
                     EELHYEPNQAAISFKQGKTMTLGVILPSLGEEFFSMAINGIEDVATQNKYTVLIGQSH
                     DNTEREVQIVDTMRRHRVDGLIVSLAKTTKSYEHFEQLEKYNIPVVFFDRVPGNPDAY
                     TVSCNLQNSSVELVDWLVQQGHKKIGFIKGPDAILHSRERLIGYQEGLKKHRIKPDTA
                     FVVATDLSVTKTNDAMHKLLALKNRPTAVIAFNDYVALDAIKYARSQGVRINKDISFV
                     SYANLPITSYMDYPPVASVEQFPYQQAEKATQILLQLITSKEAGDSIPRKVILESKV"
     gene            1164..1592
                     /locus_tag="PLO70_01505"
     CDS             1164..1592
                     /locus_tag="PLO70_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQZ73159.1"
                     /translation="MNNVSSVLTRKNFQPVTVSPGTTVIEALKIMAEKNIGSLVIMEG
                     DNFQGIVTERDYSRKVILKGKNSTETKVSEIMSKDLPIINPSDKIEHCMELMTMKNIR
                     YMPVFENNKLVGIISMSDVVKETILAQKETINQLHSYINS"
     gene            complement(1642..3750)
                     /locus_tag="PLO70_01510"
     CDS             complement(1642..3750)
                     /locus_tag="PLO70_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQZ73160.1"
                     /translation="MKLFPESAPVQLEFNKVKDLLANYCQTDHARNKARELRIHTRKE
                     FIEVDLKQSHEYRQLIVNSIYFPNDNVLNLSKELKLISIPGAVLTGEQLMLIRKLAES
                     IEKIFRWFDTERRIAYDALTRVIEGTYYEKAILELIDEVLDEFGQVKDNASGELKEIR
                     MNLYRKRNELRKVFEKIISKLNKQGYLADIEESFMSGRRVVAVFAEQKRTVKGILHGE
                     SDSRKTAFVEPEETIELNNQVHELENEERKEVYRILRELTAKLSVYASLLTNYHEVIG
                     EYDFIRAKAKLAGDIKGEYPSVTDKATVHLLEAYHPLLYLYNQRVNKPTIPVSITLDD
                     AKRILVISGPNAGGKTVTLKTIGLLQMMVQSGLLVPVHPSSQFGIFKQLMIHIGDTQS
                     IEFELSTYSSHLLHMKYFMENANGRTLFFIDELGSGSDPNLGGAFAEVILQELVKKHA
                     MGVVTTHYLNLKVMAGKTPGIINGAMAFDEKHLQPQYKLLIGKPGSSYTFSIAERIGL
                     DKNLINRARQLVDEDHFRLDKLLNRTEQDLQAIEKKEMNLNQAIKENERLKKEMVQVM
                     DKERHRQQVELLKEQNRISEEKIAYLKDMDRRLKQLLIDWRKEENKNKVIKQMEALLF
                     KKDEKKVVSKMQKKIDAKFSEIGGDIKVGNKVMMKRNHQVGEVMELRGKRAVVKIGLL
                     PMQVEMKDLVVVKEKKEEEK"
     gene            3990..6578
                     /locus_tag="PLO70_01515"
     CDS             3990..6578
                     /locus_tag="PLO70_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HQZ73161.1"
                     /translation="MKLKSLLTVLSSGLIFSAMAQNKPLMQTQTGAPGVNLDTSKTKK
                     PAGITDKVKSSKKIDGLFTLYQDTATGSVQLYVKKNQLGKEFIYQSFSISGPTQFFLN
                     QSMHRATFVFKVQKAFDKLEFSRVNTSFYYDESNPVSKTKNVDKPEAVFLSEKYVSED
                     SAGYLIAADAFFISEKMDPVKPASFPGMPPGLSFSLGGLNPQKSKYHTIRSFPNNTDV
                     VVDMAYDNPGAIVSGGPDITDPRYVRVRMQHSLIEMPSNDFRPRRDDPRVGYFMQQVT
                     DQTSISPVPYKDKINRWNLQKKDPGAAISEPIEPIVYWVENTTPLEYRQTIVDAGLKW
                     NAAFEKAGFKDAVQMKIMPDDADWDPADIRYNVIRWVSSAQPGYGAIGPSFVNPKTGQ
                     ILGADITVEWFSGSATPIIDELFSGPAADNTKQEFNIPGMDHTHYATCIMAQELKAQL
                     TTGMTVADAMDANPAEIKEMHKQFLLYLIMHEMGHTLGLNHNMKASQMLSPSEAHNKE
                     ITRKIGLIGSVMDYPAINIASDKSKQGDYYTTVVGPYDIWAIEYGYTPFAESAEEAEL
                     KKILSKSTDPKLTFGNDGDDMRAPGKAMDPRVNVNDMSSDAIGYAEERFKLVNNVMGR
                     LVQKYSKPGQSYAELRSRYGALQGQRNSMIAAVSRYIGGVYVDRSTPDQNSGNKPFTP
                     VPLATQKKAMEVLSKYVLAPNAFESDAQVFPYLQPQRRGFNQNGNGDDYRITSVILNN
                     QGAAIAHILHPSTVQRITNSRLYGNQYSVADVMNDLLKACFDADMKGNVNVYRQYLQT
                     AFVKQAAIILDPKSPGYDDVARAAALNTVKKLKTQLASAVSLNEETKAHRGSLLFLIN
                     NALERK"
     gene            6891..7550
                     /gene="msrA"
                     /locus_tag="PLO70_01520"
     CDS             6891..7550
                     /gene="msrA"
                     /locus_tag="PLO70_01520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQZ73162.1"
                     /translation="MKPFLTVAIAGLATLSSCAQQSTATDKIKKDNNYMTTQPNTAIA
                     TFANGCFWCTEAIFEELDGVISAVSGYTGGQTPNPTYKEVSNGETGHAECLQITYDTT
                     KITFDELLEVFWQTHDPTTLNRQGADVGTQYRSGIFYHNSEQKEKAEKYKTELDKSGA
                     FNNPIVTEITAFSKFYPAEDYHQQYYELNGNSNPYCKIVIQPKLEKFRKVFKNKLKVD
                     N"
     gene            complement(7584..8834)
                     /locus_tag="PLO70_01525"
     CDS             complement(7584..8834)
                     /locus_tag="PLO70_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73163.1"
                     /translation="MKLFSIRILMLFSISIFLLPSFAIAQSKYEFKGATLISGTALSI
                     GAVYKFSSVKAGVDATVTIADITGGISITNIDGGGGFDKALQPVISVPANRNGYIEFK
                     IDFFEAGTNNSLIQTEVPVTPIDVDGQMYSGLPLYEFDEVDMPNGYTYFQWAGSELNM
                     SRNGTWARGKNKAAVDYPGIDTAQKTVMFTTINANISSISIRVGADNQSNTGASRLRS
                     LYFQRFSYPFEIVLPNRTLISFNGVFKSNQVELKGVLSASHTYDKIIIEKSSNGSFFE
                     EAGNLAITNSGSGEFPFTFLDSHPYEGVNYYRIKLVNTIQKIYEISNTIMIKVGNEKK
                     MFTFYNTILRQADPAVTIQCNDQKEVQLEVADMAGRMVYSKKTKLLKGVNVVDLTYFK
                     AGRGYFVLVVRTAQEFNSQKIIVQ"
     gene            complement(9061..10689)
                     /locus_tag="PLO70_01530"
     CDS             complement(9061..10689)
                     /locus_tag="PLO70_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQZ73164.1"
                     /translation="MNRLTKYSLLFWIMLLLLTSCYNKKSHEGKIFHYNEFSGIPTLD
                     PAFAKSQATMWPAHQLFNTLVEIDDSLHIVPSLAKSWDISPDRTIYTFHLRTDVFFHD
                     DAAFEKGKGRKMTAADIEYSLKRIIDKQVASPGSWIFNRKADTIQPFKAIDDSTFQLK
                     LLRPYNPILGILSMQYCSVVPKEVVEMYGTDFRRHPVGTGPFRFVHWEEGQALVLKKN
                     ENYFERDSAGNQLPYLDGIKVSFYDSKATEFLLFRQKELEFINDIEASFKDEVLTKNG
                     TLRKNWEGKIALHTSPYLNIEYLGILVDSSNELLKDSPMKSRKIRQAINFGFDRRKMI
                     LYLRNSLGTPAESGFVPMGLPSFDSSVVKGYNYNPAKTKQLLAEAGYPDGKGMNAIKL
                     LTIAIYADMANFIAKQLEESGIPVQVEVVQKSLLLDMTSNSRAAFFRGSWIADYPDAE
                     NYLSVFYSKNPAPPNYTRYKNPVFDVLFEKAIAETNDSLRYKLYQQADQVMIQDAPVV
                     PLWYDKAVHLVQPNVTGFNPNALNLLELRRTEIK"
     gene            10752..12449
                     /locus_tag="PLO70_01535"
     CDS             10752..12449
                     /locus_tag="PLO70_01535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ73165.1"
                     /translation="MATLFFRFSFIVPLFFVMYSCAQPLNRQNQFTRQDTLRGSITPE
                     RAWWDVASYNIQVTPDYKTRTISGWNQISFDINTDGKNKKMQIDLQQPMEIDSILFEK
                     KKISTFKRLGNVYIIDFGNYEFIAARTLVKKEKAIPLHSIKIYFHGKPREAINPPWNG
                     GWIWSKDAKGRPWMTVACQGLGASVWYPCKDYQGDEPDLGALLQVRVPDTLVAVANGK
                     LHAADDQKDGTVLYTWQVKNPINNYNIVPYIGKYVLWSDNYIGETEKNLSLDYWVLDY
                     NLEKSKKQFGRDVKRMLEAFEYWFGPYPFYEDGFKIVESSHLGMEHQSAIAYGNYYLD
                     GYLGMDLSGSGWGKKWDYIIVHESGHEWFANNITTNDIADMWVHEGFTMYSEVLFTEY
                     HYGKQAGDEYLQGIRQKISNDRPLIGPYGVNKEGSGDMYNKGASLLHTIRQVINDDTV
                     FRKILRGLNKDFYHKMVDTKDVEDYFSRYSGKDLSKIFDQFLRTTEIPVLEYKINAGN
                     FSYRWANCISGFNMPVYTEKIGWLSPTTEWKDKMIPAGLIEQLNANANFYVTTKKVN"
     gene            12506..14011
                     /locus_tag="PLO70_01540"
     CDS             12506..14011
                     /locus_tag="PLO70_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73166.1"
                     /translation="MSNIRRQSIISSLVIYVGFAVGLINVYFFTKQGLFTEAQYGLTT
                     VFIAIATMMSSLSNMAMPSYIYKFYPYYKDHLPDRKNDMLSWALLTGFIGFILVMAAG
                     WFLKDLVIQKYGTNSPLLVVYYYWIFPLGLGLTIYSILEAYTWSIGKPVVTNYFKEVQ
                     WRLFTTILILLFLLNVISDFDLFIKLFAFTYPGIAIGLFIYLLITKKIHFAFTVSKVS
                     RRFLKKIILFCSFFYGAMLIQTLSKVFDSFVIASVLDDGLDKAGIFALATVVTSIIQA
                     PQRGIVSAAMPHLSRAWKEKNTALLQKVYQRSSINQLLFACAIFLLIWLNFTDGIITF
                     GLKPIYLDAAWVFFVLGITVIIDMGTGVNSQIIVTSTYWKFELISGIVLLLIMLPLTY
                     LLTKQYGIIGPAFATLVSMSVYNLMRIIFLWKKFRLFPLTVQSGYTLLLATVCYGICY
                     FAFRNIDGLAAIFLRSIAFITLYGAGAIYMRLSPDIQPVLQNVQKRLGIRK"
     gene            complement(14015..14809)
                     /locus_tag="PLO70_01545"
     CDS             complement(14015..14809)
                     /locus_tag="PLO70_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQZ73167.1"
                     /translation="MVKQFLPALAFIILLSSCSTFKPLNFTSNKQLSASEESVAPSNA
                     KFIEEISVTPQMAEVKTAQQEIGEIKKTKAVKIQNEVIKVEDNRKALKETVTREEPQS
                     SVNLFANRTPSIESASSVQLKYSILLNTEVESLPSRSLLESVDEWYGVRYRVGGNTKT
                     GIDCSGLTVAVYAAVYGMMIPRVSREQYRISRKISTTELQEGDLVFFNTNGRGVSHVG
                     VYLGNNKFIHASVSKGVMVNDLFEPYYLKRFVGAGRMDDKQAVASN"
     gene            15080..16297
                     /locus_tag="PLO70_01550"
     CDS             15080..16297
                     /locus_tag="PLO70_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQZ73168.1"
                     /translation="MQALFSYFTLLLLTAGCQNTADDKGYTKLLAASVVVRDTIVPLL
                     PPPPRPYSELEFEKKEVLAAASGTKSVLFNSKGSKLFAMNLEGMSVYEFDRASRKITR
                     EFKFKPTRGTGWDYNKSRPIASYQEKPVEACFSHDDRILWVSLHNADGIIPIWVDDFS
                     KNNAKRSANQKTKPVTVIYPGSNKKDSFQVPLIETGKTPKVISRTADNKNLLVSNWHS
                     RNVSVLEMNDTITPYGKVISTIPVSAIPRGIAVDDKTGLSYVAIMGGSSITVISNSVW
                     MKEDDMNVAASPRHLVMDTSGHLFVSYNSLGTIACLDAATGKSLFTAKTHAQPRTIVL
                     SKNYKFIFVTCYSSDFVDVYKINADNFEKVASLPCSGHPVGVDIFENDDKLEAWVCSY
                     SNGLINIYSFKKK"
     gene            16310..19984
                     /gene="dnaE"
                     /locus_tag="PLO70_01555"
     CDS             16310..19984
                     /gene="dnaE"
                     /locus_tag="PLO70_01555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQZ73169.1"
                     /translation="MKFSHLHVHTQFSLLDGAASIQNLYKKAIKDGMPALAISDHGNM
                     FGAFEFVAEAYKHKDENGNVKVKPIVGCEFYITETRHQKTFTKEQKDKRYHQILLAKN
                     EQGYRNLVKLTSLGFIEGMYGKYPRIDKELILKYHEGLIATTCCLGASVPQAILRKGD
                     EDGENEFKWWLNVFQEDYYVELQRHGMAEQDKVNDVLIKFAKKYNVKIIASNDSHYVE
                     QDDFNAHDILLCINTGEKQATPALRDFADDDVHIKDKRFAFPNDQFYFKTTEEMSKVF
                     HDLPEAIDNTNEVVDKIELLNLTRDILLPNFPVEKRFEIHSKEETIGKYKVSAESQNQ
                     WEYLKHITYEGAAKRYTELTDDVKERIEFELFTIKMMGFAGYFLIVSDFIKAGRDKGV
                     FIGPGRGSAAGSVVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKV
                     IDYVVEKYGKNQVAQIITYGTMAAKSSIADVARVMDLPLPESRALTKLVPDRPGTELK
                     RVLHAPLKAKDGEKSLEEKDGLGNDDLENVKKLREIYNQSGSPASKVLHEAERLEGSV
                     RNTGIHAAGIIIAPRDLTELIPVATSKESTLWLTQIEGTDIENAGVIKMDFLGLKTLT
                     IIKNALALIKLNHGIEINIDEIPLDDKKTFELYQHGSTIGTFQFESAGMQKYLRDLKP
                     DKFGDLIAMNALYRPGPIAYIPNYIDRKHGREAISYDVPEMAEYLEETYGITVYQEQV
                     MLLAQKLAGFSKGDADVLRKAMGKKQKAVLDKMKAQFIAGATEKLLPPEKLEKIWTDW
                     EAFAQYAFNKSHSTCYAYVAYQTAYLKAHYPAEYMSAVLNNAGSIEKITFFMEECKRM
                     GIKVLGPDINESLKGFAVNDKGEIRFGLGGLKGVGEAAVESIIEERKKGGTFTNIFDF
                     IRRINQRTVNKKTLESLAYAGGFDCFPEHHRAQYFTIPEGETVNGLERVIKYGQVMAS
                     QNATTTNTLFGDLPISMEIPPPRITDCAPWPLIVQLDHEKEVTGIFLSGHPLDNYKFE
                     MKHYGVTPLADFNEFKEAIKMQPNPGRPFRIVGLVSDAQHRIARSGNKYGNFVIEDYS
                     GKTEFPLFSEDYMRLTPILQQGSTVLIHGYFKQRYNKDEFEFKVMSVSLAETMKRNMT
                     KQLTIEAHPQDISSELVNFIEKNVKNNPGKSTLKFTLSEPKNKMKISLVTMNSGFEMN
                     EEMIQFLENKPELEVQVVTS"
     gene            20146..20463
                     /gene="trxA"
                     /locus_tag="PLO70_01560"
     CDS             20146..20463
                     /gene="trxA"
                     /locus_tag="PLO70_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQZ73170.1"
                     /translation="MAKEFTDSNFQSDVLASDKLTVIDFWAEWCGPCRAIGPVIEELS
                     KEYDGKANIGKLNVDNNPEVSMNYGITSIPAILFIKNGQVVDKLVGAQPKANFVKKIE
                     AHI"
     gene            complement(20531..21790)
                     /locus_tag="PLO70_01565"
     CDS             complement(20531..21790)
                     /locus_tag="PLO70_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HQZ73171.1"
                     /translation="MNLKGRLTVLSFLQFFVWGAWLITIATYFFSNNMGTGAQFGAIF
                     STLAISSLIMPALTGIIADKWMNAERLYGLLHILYGAVLLYVPQVKDAGTLYYVILVA
                     MLFYMPTISLSNSISYTLLKQNNYDVVKVFPPIRVWGTIGFIVAMWMTNLTGSKANAN
                     QFIIGGVAAILLGIYSFTLPKCPPQRSIAKDASIIEQLGLNAFRLFAKYKMALFFIFS
                     MFLGAALQLTNMYGDTFLDDFRKVPEYGPESFVVKYSTIIMSISQISETVFILTIPFF
                     LKKFGIKKVMLFSMIAWVLRFGLFAYGNPADGLWMIILSCIVYGMAFDFFNISGSLFV
                     ETTTDSSIRSSAQGLFMMMTNGVGAFLGSKISGYVIDKYFTVNGADKDWQNIWLAFAA
                     YALVVAVLFAVLFKHKHNPDVIEKVTH"
     gene            21987..23129
                     /gene="mqnE"
                     /locus_tag="PLO70_01570"
     CDS             21987..23129
                     /gene="mqnE"
                     /locus_tag="PLO70_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HQZ73172.1"
                     /translation="MITTTAISPELQSIAEKVKAGQRINDEDCLLLFEKASLGFAGSL
                     ANAIKEKLHGDKVFFNRNFHIEPTNVCVFSCNFCSYSKLYAHREEGWELSIEQMLHMV
                     KKYDGKPITEVHIVGGVHPKMDLQFFADLLKAIKTHRPELHIKAFTAVEYDYMFRKAK
                     VSAEEGLKFLIAAGLDSIPGGGAEIFHPEIREKICADKVDADGWLNIHETAHKLGLHS
                     NATMLYGHIEKFEHRIDHMRRLRELQDKTNGFNTFIPLKFRNKDNDMSNVPESTHIED
                     MKMYAIARIYLDNFPHLKAYWPMIGRQNAQLSLSFGVDDIDGTIDDSTKIYSMAGSEE
                     QSPSMTTAELVTLIRQAKREPVERDTLYNEIKNYSNVAAEVNAGLN"
     gene            complement(23251..23805)
                     /locus_tag="PLO70_01575"
     CDS             complement(23251..23805)
                     /locus_tag="PLO70_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73173.1"
                     /translation="MATKKKAKKKPAKPTPKRKATAKKKVTKKKAAPKRVKAVKKKVV
                     KKVVKKKVAKAQPKRKAAPKKKVAPKKAKAAKKKVVKKVVKKAVPKKAMPKKAVAVKA
                     KVVKKKSPARTVKVAQPETKAPVSVIPVMVEEVKDIYITPDGELEVTETIITVDDDDV
                     VGTGFEEDDIAEADDEKEEDEPGV"
     gene            complement(23916..25343)
                     /locus_tag="PLO70_01580"
     CDS             complement(23916..25343)
                     /locus_tag="PLO70_01580"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HQZ73174.1"
                     /translation="MPYEAISVFDIFKIGVGPSSSHTLGPWRAAQRFVQSLMQENRLT
                     ETTQVRVLLYGSLAKTGKGHGTDVAVQLGLCGDDPVTFAVDQIDSKIRDIAAMKKMVL
                     LGMHEVDFDPQEDIEFLVTETLPYHPNAVTFLATMRNGESIAETYYSVGGGFVIKEGE
                     EAGTVQTVQLPFPVNDADDLLHWCIKTGLSIHEIVMENETAWRGEEETRNSALNIWRV
                     MKECIYRGCHTPGILPGGLNVKRRSAALNKKLLSGKEYHDYDSWLMAIRSGGNDFKYT
                     LDWVSCFALAVNEENASFGRVVTAPTNGAAGVIPAVLQYYISFCNGNNEDKIIQFLLT
                     AAEIGSIFKKGATLSAAMGGCQAEIGVSSAMAAAGLTECMGGTQKQVMMASEIAMEHH
                     LGLTCDPIAGLVQVPCIERNTMGAIKAITASQLALQSTPDFAKVSLDAVIKTMWETAL
                     DMNHKYKETADGGLAVNVPLSLPEC"
     gene            complement(25440..25907)
                     /locus_tag="PLO70_01585"
     CDS             complement(25440..25907)
                     /locus_tag="PLO70_01585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQZ73175.1"
                     /translation="MKKIALFLSVSFILLLNSGCLKDDSCNAKTVESEQAAIVNYALA
                     NGMTTTAHSSGLHYQVINAGSGPAPTINSTVSVRYTGKLLSGTIFDSQTGTPITFPLT
                     QVIQGWQIGMPLIQEGGTIKLILPSSLAYGCTGYGSIPSDAILYFEIELVDVQ"
     gene            complement(25980..26297)
                     /locus_tag="PLO70_01590"
     CDS             complement(25980..26297)
                     /locus_tag="PLO70_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73176.1"
                     /translation="MLTKHETDFISYWEKNRLVKKKVLRQIYVGLPLAVILVVAIFAN
                     LLSGWYGRAQMVMFRENASLIVILLVAALSIVVFIVIFSARHRWDMNEQRYRELLSRK
                     EES"
     gene            26386..27327
                     /locus_tag="PLO70_01595"
     CDS             26386..27327
                     /locus_tag="PLO70_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73177.1"
                     /translation="MKITLLLAQYLYTHKRLDLPGIGTFHLDPTVAIEPEPDKNTKKD
                     FGESITFEHNPAIKENQALIDFIASHTGKIKALAAADLDSHLSLGLQFLNIGKPFMLE
                     GIGSLVKTQAGQYAFTSGQLMPEVLKEQPLKQTHPAEIQEEAFSSYKDILRPRKEKMQ
                     WKKPVAFFLVVAGIALAIWGGYTIYKNSSEENETVSTVEKKKIEEVIPATMIMDSTAT
                     KDTLLPKKEDVAGPQPMTVPVGNYKFIVETAGRERAFKRFKLLKSLPTNIQLGTSDSI
                     TFQLFFVLPAAVADTARIMDSLRIHYTPKWSRAYIAQ"
     gene            complement(27461..30040)
                     /locus_tag="PLO70_01600"
     CDS             complement(27461..30040)
                     /locus_tag="PLO70_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQZ73178.1"
                     /translation="MKAIKQLFILYWVLLSSPVLAQVKLPVLHDSLFSTYYHQRLSLF
                     ELTPVNPGDIVFLGNSITDGAEWHELFGDSKIKNRGISGDISAGVLYRIAGIAKQKPG
                     KVFLMIGINDLARGVSTDSLLKNILLTAEYFKQLSPSTKLYIQSILPVSNQFGKFIGH
                     ASKTDSILKVNIRLSKEAASYRYTYIDLYSNFCNRLSKLAPKYTNDGLHLLGEGYILW
                     KHLIFPYVYQLQEKPSLLPLPQKITWGYGYFPLYSCKVIVVNDKKLTGEVEDLKKLLA
                     RTGRQPEVKEKARKDEVYIELRLEKSEQPGISMESYTLSVSKNRILLSAFTLHGIFNG
                     IQTLRQLMRDGVMIEACEITDTPAFSWRGYMIDVGRNFMSMDLLKQQIDIMAAYKLNV
                     FHFHATEDIAWRIAIKQYPELTTPETMLRNKGQYYSEAEIKELIAYCKERYITFVPEI
                     DMPGHSAAFKRAMKTDMQSDSGLLYVKNILKEFCTTYDVQYIHIGADEVKIINKNFIP
                     EVTKYIESLGKKVIGWQPGGNFTDNTIRQLWMDDNSHLSSNKNIQYIDSRHLYLNHLD
                     PLESVVTIFNRKICEKEKGDSLAIGATLCMWHDRKVGKEEDVLTMNPVYPGILTFAER
                     AWKGGGQSGWISNIDDGDKEGFINFESRLLEQKALYFKLKPFPYVKQSNLLWHLYGPY
                     YNGEDLFKTFEIENTLLHPDSEKSAKQIIGGTVVLRHWWAPLIKAALEKPEEKTTWYA
                     TTTIWSDEEATKNFWIGFNNISRSPATDSPPRDGWDSKGSVVWVNGKLVSPPNWKRAG
                     QKGHSEIPLIDEGYEYRTPTSIHFKKGINSVLLKVPIGSFKGKDWQNPVKWMFTFVEI
                     NQE"
     gene            complement(30073..32106)
                     /locus_tag="PLO70_01605"
     CDS             complement(30073..32106)
                     /locus_tag="PLO70_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQZ73179.1"
                     /translation="MNIKYSYIAIIIVLSFNTTTFAQRDTITLNSDWQFSIDKKAEGF
                     EGKWNEIPLNDARIVRLPHTWNIEKDNQNHYGWGWYQKRIKVPANWKNKNVVLLLGAI
                     NHTCYIYINGQKIAENIGAGFDKFFVSLNGRVKYGKDNTITIAVNNDYGKNKVPFGSS
                     FDWPNDGGIIRPVALIVSGLPAAAYIHATPVLNVADSSGKLKIKLGFETIVSKNVELG
                     ITITEENQPTKNIVLQSTIKPVWQNREAIIEYNFAKVHPWHFDLPNLYRVDVTVLSGR
                     KVTDKISTHIGFREVKFESGQTFLNGERIKLMGTEWTAGSNPDFGFAETDSVIIAMGK
                     LLKNVNSIFSRLHFQQADVFYDFCDRNGIIIQQEVPLWGPETPSNDTIRSIALKQLER
                     MVYNYYNHPSIFAWGVGNELRGRDSDMKKMIADLIAKAHTLDPSRHVAYVSNTLTSTF
                     FNNPRFTPDAASDGDYIMMNEYGGSWWDVPVGKIHLYLDSVHISYPDKPFFISEFGLC
                     EPNFKGGDERRLEDLIYHMAIYESKPYVEGAIYFDLTDYRTHYLGTSEKNKFRRRVHG
                     IYDMYGHPKPSMKILRELSSPVEVQQARQWKKGKLNLLIFGSIGLPQHTVSGYKLYIS
                     EKADNYATAKVYDLPVIKPGQRINFEVDDLFEGKCIVTIVRPTGYIVTQKSFY"
     gene            complement(32158..35343)
                     /locus_tag="PLO70_01610"
     CDS             complement(32158..35343)
                     /locus_tag="PLO70_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQZ73180.1"
                     /translation="MRHHKMLRTPFILVIILLASNCVIGQLPAELQTPELVSINRMPM
                     RASAFGFENIELAKGFDKEKSGNYFSLNGNWKFNWVKDPDQRPQQFYALNYDDSQWDD
                     FRIPASWELNGYGLPIYVNQPYDFAGHNLRYGKMNPPFDIPANNNPVGSYRKIILLPS
                     AFKGKQIFLHVGNAKSCLFVWVNGKKVGYSEDSKLAAEFDITNFVKEGENLIAFQVYR
                     WSDASYLECQDMFRFSGIERDVFLYCTNKLNIRDANIEATLDNSYTNGFLSVNTEIDN
                     YKLEKGYLSKMDSFTLELQFLDPAGKIVYQDKTHDFKTVLGNYKTQVQFSTVITNVMS
                     WNAEIPNLYTLFIILKDKDGNMLEVVPQRIGFKKVEIKGTDFLVNGQRVFLKGVNRHE
                     HHPRKGHTLSKDDMRKDMEMMKRLNINAVRNSHYPPDPYWMELCDEYGLYVVDEANIE
                     SHGLGYDLSITLGNKFLWREAHLQRIQRMYERDKNHASVVTWSLGNEAGDGSNFYAAY
                     DWLRQQTNLPIQYERDIDYGKPNNTHFSEIFCPQYVSPGGMLRYSTSDAKIPHIQSEY
                     AHIMGNSLGNFQDYWDVIEQNPKLQGGFIWEWIDQSIDTVKNGKRIKAYGGDFPLEGP
                     VDENLSDNNFCVKGVVDAYRNLTPMAGEIKKVYQHIKTKWKGNNSVNIKNGYFFKSLD
                     NVMLRWVLQENGLSKETGTIGSIALLPQQENDILIPIKSVQQSDKEYQLNIYYVLKTD
                     EPFLPKDFEIAAEQFLLKMLPAGTVNPSTRSTIKIQQNEVQCSINGKNFSILFDLKNG
                     ILVNYKLNGEEVMQQGPIPSFWRAPTDNDIGAGFNRSLREWRNAYENGTAITTTITKI
                     SEEQCQISFTKEVVNGDAKHRQVFTIYSNGTVKVDNYFNALRGKHLLLLRAGNDVTIH
                     GSFKNIQWYGRGPGENYWDRKTHTFLGQYKQTLDEQYYPYARPQESGNHTEVRWVTFS
                     NARGRKIHFAFADSLLSFSALPYRLDDLDPEINKKQYHSGELTKRDLAYLHIDLQQTG
                     LQGIDSWGSMPLVQYRVPFKDYRYSYWMNFE"
     gene            complement(35396..37690)
                     /locus_tag="PLO70_01615"
     CDS             complement(35396..37690)
                     /locus_tag="PLO70_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HQZ73181.1"
                     /translation="MKIKFIFILSLTTTLCVAQQTVNIIPQPVSIQQQSGNFILTGNT
                     AIQYNPQQKGLKKVAKFFNLQLKNTTGFVLPVNTSLPATIQLSIEKLATIGDEGYNMD
                     ITSKAVTIKANTTAGIVYGIQSLLQTLPAIRTNAKLEIPCMSITDYPRFKYRGMHLDV
                     SRHFFGPELIKEYIDLIASYKMNVFHWHLVDDQGWRIEIKKYPKLTSVGAWRVDQTDK
                     IWGSRPQAKPDEKPTYGGYYTQEQIKEIIAYAAQRNVTIIPEIELPGHVASAIASYPQ
                     LCCKQQQQFPLTGGNYTGISSNYCAGNDSAFNFLENVLTEVIDLFPSQYIHIGGDEVD
                     KNPWKKCNRCQARIKKEGLKNEEELQSYFIKRIEKFVNSKHKKIIGWDEILEGGLAPE
                     ATVMSWRGENGGIEAAKQSHDVIMTPGKPVYFDHYQAGPEGEPIAIGGMNTLKNVYDY
                     EPVPKELNAEEAKHVLGAQANLWTEFITTAEHVEYMILPRMPALAEVVWSPKENRNWN
                     SFNERLQNHFKAYGEKGLHYCPGNFTVNIRPFSQNGKLFVNLSTEILNGEVYYTTDGT
                     EPTLTSNKYSAPVEINSTGLLKASTMQNGRIMGVQAARQSFAMHKGIGKNVIYANPVS
                     RYYMADGSNSLTDGVRGTGTVGKFWHGFSGKDLIATIDLEEAKNIQSLALGCLQNYND
                     WIFLPQSVKFETSVNGTDFEEVQTVNNLISINEKNAKFDFKTSFPAKTVKFIRVTAKN
                     NICPPGHNGEGKPGWIFADEIIVD"
     gene            complement(37687..39090)
                     /locus_tag="PLO70_01620"
     CDS             complement(37687..39090)
                     /locus_tag="PLO70_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQZ73182.1"
                     /translation="MLLQRNKPIKVWGKGIPGRSVNVLFSKEKKTTEITNDSVWIVTF
                     PKQKATTHSQILSVTSEAEQIEFKNILIGDVWICTGQSNMEWPMVKEQHWKDEIHSAE
                     QSNIRLLNPPPAGRNVFGVPYTDSLISRLTTERFYDWNGWQQCDSISVRLMSAVAYYF
                     AKKVFSETGVPIGIINLSIGGAPLETFISTEALSQQVTFRKKVNGNWLSNDALPIWIR
                     ERGTQNVGKNNNVPADEQGPNHAYKPGFAFKAGIDSLRNFSVKGILIYQGESNAEEPE
                     RVDEYKELFKLFIKEYREVWSNKQLPIYWIQLSSIERKYWAMFRNNQLQLLKETHNTG
                     MAVTSDIGLKNDVHPPDKKTVGERLARWALKKTYYKKIIPSGPLPLSAKYRNGKVIVS
                     FQYMANGLKTSDDKPLKGFSIDGKNETKAIFGDRGIIIPVSSKPQVIYYGWSPFSNGN
                     LVNSENLPASTFKIKVQ"
     gene            complement(39171..40226)
                     /locus_tag="PLO70_01625"
     CDS             complement(39171..40226)
                     /locus_tag="PLO70_01625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQZ73183.1"
                     /translation="MTEFVLSTYGLQKDELTVEEFGSGLINSTWKVSAPGKQYILQRI
                     NSNVFQEPAAISGNISLIANHLKNNYPDYLFAAPIISNDGNEMIYVEGNGYFRLLPFI
                     EGSHTFDVATTLEMAFEAAIQFGRFTRLLSGIEIDKLEITIPDFHNLSLRYLQFLSAA
                     ANTTKLRNQETFELIETLKEFSFIANEYEKILTNPEFKKRVTHHDTKISNVLFNHNRK
                     GLCVIDLDTVMPGYFISDVGDMMRTYLSPVGEEESDFSKIIIRKEYYTAIVEGYMSEM
                     KDELTEIEKKHFFYAGKFSIYMQALRFLTDYLNGDEYYGAAYPGQNLVRASNQTILLK
                     RLMELAPDLYHIAIDSR"
     gene            complement(40244..41596)
                     /locus_tag="PLO70_01630"
     CDS             complement(40244..41596)
                     /locus_tag="PLO70_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73184.1"
                     /translation="MHRKQFIKNTTLAAASLAILPATSLFAANQKDKIKLVIIGVGLR
                     GQNHLDLVLRRKDVDLIAICDIDDRMLSRSKEMITKSGKKMPQIFTGDPYAWKKMLEL
                     KDIDGVIIATPWEWHKEMIIGSLQAGIKYIGTEVMLGITLQDHWDVVKATEQYNAQVM
                     MLENVCYRRDVMAALNMVRQGLFGEMVHLQGGYQHDLREVKFNNGIDAYGKGCEFGDK
                     GWSEARWRTHHSVYRNGDLYPTHGIGPIAHYININRGNRFVNISSFSSKARGLHEHIV
                     KKCGPEHPNAKINFKLGDVVTTSIGCANGETILLQHDTNLPRPYSLGFRVQGTNGIWM
                     DVNKGVHIEGQSKPHQWDAAKEWFDKYDHPLWVRWSKETEGAGHGGMDFFVIHSFVES
                     IKRNIATPMDVYDAAAWSAITPLSESSIEKGNETIEFPDFTGGKWMYRKPVFALNDEF
                     "
     gene            complement(41710..43062)
                     /locus_tag="PLO70_01635"
     CDS             complement(41710..43062)
                     /locus_tag="PLO70_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HQZ73185.1"
                     /translation="MKKAFAVFVLSIVVLSACKKNNDGGTTPPPPPPPPPPPVVVLNT
                     ADSILLVNETVSGSKEATRLAQNNPASDFYTTGFYLPASTVMTVEVTAENGTRLPTLL
                     VGTYSRYQASWNPTSHTLTAGVNSITDAIGGIIYVRFHNDNPTGRVRLKFTSGMRPVP
                     YYQLGRTTQADWVKMVDNINNVPDVQLVGNKTIITFSLVNARTYKSENQEALIKKADR
                     VIAIEDSISGLFGNDPIDQPNVHKYLMTETDHPGYFMAATFYRTFYSSVTGGVPAILK
                     ADNLTWGPWHELGHMHQQGSWTWSELGEVTVNIYSIAVEKAFGILPTRLTTQNEWNNT
                     AAYLARPEMERNFNGSNASVWIRLCMFQQLKLAFGESFYHELHRQARRETNRPTTTDT
                     RMRWFMLKACNISGKNLTSFFQKWGMKLSTQSATDAVFTEITALGYPAPAQDITLLTD
                     "
     gene            complement(43073..44257)
                     /locus_tag="PLO70_01640"
     CDS             complement(43073..44257)
                     /locus_tag="PLO70_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQZ73186.1"
                     /translation="MYSQEDLIALKGFYSNQLLTNTIPFWFPRSFDKECGGFLLMRDA
                     DGSLIDDDKAVWIQGRATWMLSTLYNTVEKKQEWLDGAKLGYDFLNKHCFDKDGQMFF
                     HVTRDGRPIRKRRYFFSETFYVIAAAAYAKASGDEEAAGKARFVFGKCIEYATIPGLL
                     QPKFTNTRPSKGIGVPMIMMNTAQQLRETIGDPRCDEWIEKWMAEIERDFVKDYIQCV
                     MEQVAPDGSIIDHIDGRTLNPGHAIEGAWFILHEAKYRNNDKRLIDLGCRMIDYMWER
                     GWDKDYGGILYFRDLYNKPVQEYWQDMKFWWPHNEVIIATLLAYLMTGNEKYAGWHKM
                     VHDYSYSHFHDKENGEWFGYLHRDGTLAQSAKGNLYKGPFHLPRQEWYCMLLLENYLK
                     KR"
     gene            complement(44264..45832)
                     /locus_tag="PLO70_01645"
     CDS             complement(44264..45832)
                     /locus_tag="PLO70_01645"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HQZ73187.1"
                     /translation="MQKLIFNAGLCFLFLLLSSFECSKKSTAPPPAAIQASAYAPATP
                     VLKGLTANPVLRVAVSIPSGNSEQQYKKIICTLNADAISNIQKIDVYLTGGEPFSIAN
                     PVTSVNPSAASFDLAVNLNLKPGIHFIWFSIVLKSDATIDNKIELRCTKLIDNANKEL
                     LVKQDNSVYAKRTGVAIRKAGDDNFNTYRIPGIATTDKGTLLSVYDIRYNNSADLPGN
                     IDVGLSRSTDSGRSWQPMKVIMDMGAPHENNGVGDPSILFDPVTKKIWVAALWSKGNR
                     SIAGSLPGISPDTTGQFVLVSSNDDGLTWSAPYTITPQVKNPAWHLFFNGPGSGIAMQ
                     DGKLVFAAQYWNETKMPYSTIIYSDDHGTNWKGKVNGPKSNTTESQVVETEPGTLMLN
                     MRDNRGSFRSVATTTNLGTTWTEHSTSYNALPDPVCMASIIKAKVMVKGIMKDVLFFS
                     NPSTKSGRYNITIKASLDLGQTWLPANQLLIDERQCYGYSSLTKIDDNTIGILYEGTK
                     DLYFVKVSVNEIIK"
     gene            complement(45842..47083)
                     /locus_tag="PLO70_01650"
     CDS             complement(45842..47083)
                     /locus_tag="PLO70_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ73188.1"
                     /translation="MIQSKKYPWIVVALLWVVALLNYMDRQMLSTMKPSMQIDIHELQ
                     TAANFGYLMAIFLWIYGFMSPISGIIADKINRKWLIVGSLFVWSAVTFTMGYAKTFDQ
                     LYWLRALMGVSEALYIPAGLSLIADYHSSKTRSLAVGIHMTGLYMGQALGGFGATIAD
                     KFSWQQAFHSFGIIGIVYSVVLIVLLREKRVVDEINTENNNLEKIKTPLLKGLGILFT
                     NISFWIILFYFAVPSLPGWGVKNWLPTLFAQNLGIDMSKAGPISTITIAASSFIGVIF
                     GGILSDRWVQKNLRGRIYTSAIGLALTIPALLFIGFGHSLFAVIGAAFCFGFGFGMFD
                     ANNMPILCQFVSKKYRATAYGLMNMVGVFAGAFITDLLGKSTDKGNLGKDFAMLAGIV
                     LVALIIQLSFLRPKANDFTEE"
     gene            complement(47080..48237)
                     /locus_tag="PLO70_01655"
     CDS             complement(47080..48237)
                     /locus_tag="PLO70_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQZ73189.1"
                     /translation="MKISVIVKLMFLTIPAVAQKNPNLLLKWKIVGELPADSGQQISL
                     GVAGPVTGYHKNVLFIAGGANFPAGMPWEGGVKKFYTTVNVYFKKRHKLKLSSQSFSL
                     PYSIAYVASCSTPFGVLYAGGESEKGISDKVWLMIWDDRAGEILFKSLPVLPVAVSNA
                     SAILNGTTVFIAGGETAAHTTSQLLALDLVNIQAGWNKLADIPQPVSHTVVTAIPGKT
                     GDKIFLCGGRKKNNSGISDFYNSVFVYDVITNTWEEKKALPYALSAGTGIVCDAENIL
                     LFGGDKGIVFHKTETLIAAINAEKDPVKKQELIQEKNKLQSEHPGFSNEVLIYNIEND
                     AWNIAGTIPFDTPVTTTAVQWKSGIIIPSGEIKAGVRSPNILGVKILQKNK"
     gene            complement(48248..49195)
                     /locus_tag="PLO70_01660"
     CDS             complement(48248..49195)
                     /locus_tag="PLO70_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQZ73190.1"
                     /translation="MVRQHLQGLIAAPFTPMHSDGSLNPGLIPLYYSMLKSNGIKGAF
                     ICGSTGEGVSLTINEKKAIAEAWAACTREDSDFLVMPLLGGTCLADCKELALHAREIG
                     LDAVSFTAPFYFKPANVEMLAQCCAEVASMVPGMPFYYYHIPVLTGVGFTMFDLLKAI
                     DGRIPNFAGIKYTHEDFMDFLSCINFENGKYDMLWGRDENMLPALALGAKAAVGSTYN
                     YAAPLYYDLIDAYNTGDLAKAQFLQQQSIDMIRLLGKYGGIATGKAFMKLINLDCGDF
                     RLPVKNMNSAEFESFKKETAQLGFSTFCSAIPVDSTAIN"
     gene            complement(49290..50816)
                     /locus_tag="PLO70_01665"
     CDS             complement(49290..50816)
                     /locus_tag="PLO70_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ73191.1"
                     /translation="MKHNKFLILTGAFLLMLSVLSCTKKLDEVVPQDTISKDQALKDP
                     NAARTLYHGVYGLFRSYSGTFFQLGEMRSDIWVDGLFTESVDGGLQNLYRHNISTLNV
                     PFTNWGGFYNLIYNFNNVIKIIPQTPLPDAEKTRILAEVYGLRAYVYYTMLKTWGAVP
                     LNTEPVETINNTAETYKPRTAADSIMIQIKSDIEQSLQLFGSANTLSTAKRVYWSRVA
                     SLVLKGDVFIWSGTHMGGGTTDLTTAKNALQEVRDLQGSSLGLQANYAHIFDPTRKVN
                     NSEIIFAINYELQQAQNGIFGSFLVNSIQAGTLSFTQAPTPTVSSVYPYVNSANRVGM
                     NQAMINRLTALPADQRISNTFRVMYSTAPPHAIRGILLTKYIGTTAGTSQIYNNDYPI
                     YRYADVLLLLAEAKAKLGEDPSAEINAIRLRAYGAGYTPYVNGSITENMNAILEEYLK
                     EFIAEGKRWWALRRAGDSYVYANLNPTYFSPASTAKFLLPLSQGMLTADPLLTQTSGY
                     "
     gene            complement(50847..54197)
                     /locus_tag="PLO70_01670"
     CDS             complement(50847..54197)
                     /locus_tag="PLO70_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ73192.1"
                     /translation="MKSACLKGWLLIVSFLISATVWSQVQKISGKITSSDDNKPLPGV
                     SIIIKGKTVGTQTNINGEFSIDVSQGEVLVFSITGFTTREVKVGANTTIDLSLETKVS
                     ELDAVVVTGYGTQKRKEVTGAVVTVNPKTFEHSPSTNVATVLQGNAPGLRIQQRTGQP
                     GTTPAISFRGGTEFGGGGTPLFIVDGVIVPSLYGLDINDVASIDLLKDAATTAIYGAR
                     AANGVVLVTTKKGKKGKAQVTYSFSHTTNFVRTNPSEYLSAADYIRMNRLGIQARFRG
                     DSLDNNINAMNTDRNQLLGAWGWAVNTGWRSAEGLYTTQLLNNQNRHLLNNARWKLLV
                     DPNPFNTSQLDSILFTDLSAKEREAMLLRQVNTKEHNINFSGANEQGSYALSLGTVKD
                     NGMIIGSLLKRINLNFNGGLNVGQNLKITTNIGAYNVEQALPYGQFGNVDPEGGAAGG
                     LLQRFVGVAPTVRYVNDTSGAILPGPNDVTLGNPLYWSNLTVNSTNQQRFLGGINLEY
                     TVLPFLKILASGSGYYQYTNNNFFTKAYQQGNGGAFNTNRAASFNNTKTTQYTTNAFL
                     QFTKSFKGHTVTALAGGEFYDFKNYVNSGFSQGAPTDLIPWLTASTPPSVQGTTIVNP
                     AGASSNFNQWERITSAIGRVNYTYKNRYLLTGVVRVDGSSRLKKGNYYGTFPGVSVGW
                     NLHNENFYQGTFISKYLSSVKPRISYGVNGNVSSLGFFATSQVYNNAGTYNGFGGTYA
                     GSYINSDVRWERTNSLNFGADLGFLNERITLTADYFIRNVFDKLAGLNISSQTGFGSF
                     TTNLGQLQNKGIELAVNAKIIESKNADGFSLEAGVNYFHVKSFAKKLPFNGLPGNRTS
                     GFFVWDPKNPGQQMYVRGLVEGQRIGLDEVWAPKWDGIYTDLSKITADANVYNAFLPY
                     TNKRIKQLGDAQWHQVNKNDTIDSRQFVKVGRTTPQHIGGFNISSSFKGLRLYAGFDY
                     AFGFVILNNQIVRGLSQVQGSQNSTTDVLKTWSPTNPNGTMPRYYWANQGRNYATDAS
                     GNNPAANLWEKGDYVMLRELTLAYDLSSQILSRYLGNRLKGLSVNVTGTNLVYFSGYS
                     GNFPEVGGFDQGKFPLPKRLTIGVRVIL"
     gene            54370..55083
                     /locus_tag="PLO70_01675"
     CDS             54370..55083
                     /locus_tag="PLO70_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQZ73193.1"
                     /translation="MMEKATTDTFKSIENTSLVDKVEANLVELLQQQKLKIGDSIPTE
                     LELAEKLGVSRTVIREALLRLRMMGLIESKKKKGAVITSPDLFGNMSKSMNPHILAPE
                     TLKEVFEIRLVLEIGMADLLFNRITKEDLKALHEIVDDEPPATQYHLFNIEHEIAFHG
                     KLYEITGNETLKKFQKMLLPVFDYVHNSGLLKKQSLLKMFVSHKELVSILENGTPEDF
                     RNGMRNHLENHFARLFDLS"
     gene            complement(55280..55350)
                     /locus_tag="PLO70_01680"
     tRNA            complement(55280..55350)
                     /locus_tag="PLO70_01680"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55316..55318),aa:Cys,seq:gca)
     gene            55467..56345
                     /locus_tag="PLO70_01685"
     CDS             55467..56345
                     /locus_tag="PLO70_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQZ73194.1"
                     /translation="MSRIAESELIINDRGAVYHIDLRPDELGDTVVTVGDPDRVREVS
                     KYFDKIEVKRQHREFISHTGYIGKKRITVLSSGIGPDNIDIVINELDALVNIDFETRE
                     VKKQLKSLNIIRIGTSGSLQADIPVDSFVASTHGLGIDNLLNFYRYEQNDEEQQLIHS
                     FVTHTQIHGQIGHPYITSGAASLIKHFVKDFYHGITVTCPGFYGPQGRILRLGIRNPE
                     LINRLTDFRFGQHRITNFEMETSAIYGLGKLLGHNCLAVNAIVANRIKKEFSKDGKAA
                     VENLIKKFIQLFSENI"
     gene            56345..57133
                     /gene="dapF"
                     /locus_tag="PLO70_01690"
     CDS             56345..57133
                     /gene="dapF"
                     /locus_tag="PLO70_01690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQZ73195.1"
                     /translation="MQFHFYKYQGTGNDFIIADNRKNEYSSLTTGQIHHLCDRRFGIG
                     ADGLMLLNEKEGYDFEMKYYNADGKEGTMCGNGGRCITKFAYHLGIHREVYHFLAADG
                     PHEAEIDMNGIVSLKMKNVDVIKKFQGDLVLNTGSPHYIKMVRNVMDIDVYKKGHDIR
                     YSREFSEEGINVNFVEQTENPNKIIVRTYERGVEDETYSCGTGVTAAALVSYHNENGF
                     NDIEVKTLGGTLTVEFDRFDDERYENIWLCGPAEKVYEGAIEIK"
     gene            57234..58172
                     /locus_tag="PLO70_01695"
     CDS             57234..58172
                     /locus_tag="PLO70_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQZ73196.1"
                     /translation="MIQYFKNINQQTVEIDKPEIGTWVNVLPPLKQEEFSELSTGLDI
                     PIDFLTDSLDIDERSRFEEEDNVKLIVIKTPTENNSFNESDAYYITIPICIILTHNQI
                     LTVNSFDNPAIKKFLNTFQNRHPDKKNMMVLKVFEKVVQNYMDHLKEINQRRNIFEQK
                     LYAASRNEHLLELMRIQKSLVYFVTAIRSNELLFMKLQRTNFLSLNEEEKEFLDDLIV
                     DNSQALEMANIYSNILSSTLDAFAGIIANNQNEVLKRLSVITIVLTLPVLVASIYGMN
                     VPIPYQESKLAFYMPVGLSLIISLFIGWFFLKKKIF"
     gene            58181..58645
                     /locus_tag="PLO70_01700"
     CDS             58181..58645
                     /locus_tag="PLO70_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQZ73197.1"
                     /translation="MNRFNIRVYGLLINEQKQVLVSDELIRGSYITKFPGGGLEFGEG
                     TRNCLQREFMEEMNLQVEIGDHIYTTDYFQLSAFNPEHQIISIYYFAKALEEIKVPLR
                     DKPFDFDEEQLKVYEATKETETFRFVDWHEFSPESVTLPIDKIVASIIKERY"
     gene            complement(58642..59442)
                     /locus_tag="PLO70_01705"
     CDS             complement(58642..59442)
                     /locus_tag="PLO70_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQZ73198.1"
                     /translation="MIHTIIRSKPTRLFIILAGIFIANALIAEIIGVKIFSLEKTLGF
                     PPLHIRLFGNDLSVNLTAGVLLWPVVFIMTDIINEYYGMKGVKFLSYLTAGLIAFAFI
                     VFYGAMKLTPADFFITSKQGSGVPDMEKAYNSVLGQGGFIIIGSLTAFILGQLIDVFV
                     FHKIKKVTGEKRIWLRATGSTLISQFIDSFVVLFIAFYVGTRVNAAGNDFVWPFSLFI
                     AVGVVNYIYKFIVAIILTPVIYLVHNRIESYLGQEQATAMKKAAMGDD"
     gene            complement(59417..59725)
                     /locus_tag="PLO70_01710"
     CDS             complement(59417..59725)
                     /locus_tag="PLO70_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73199.1"
                     /translation="MRWLLFLSRLAFISGVFFLASASLLVTDWLKDENLKSTVIIIGY
                     FMGIILFPVVNLCYIAVFLVKKRIRQYVPLWLIVSNIFFLLALAYFIFYLNDPYYHQK
                     "
     gene            59780..62257
                     /locus_tag="PLO70_01715"
     CDS             59780..62257
                     /locus_tag="PLO70_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQZ73200.1"
                     /translation="MNNPLLKKILPHAIAILLFLVVSALFCKPALEGNVLNQHDNVGW
                     KGMAQNAFEYKEKNGHFPLWNPNLFSGMPNYQVAMEGKSILPDTVKILSLGLPKPMNF
                     FFLACLCFYILCLVLRVNPVIGMLGALAYSFSTYNPVIVAAGHDTQMLATAFMPLLMA
                     GIISIYEKKYWLGFALTAFGTYQQISVNHLQVSYYFFLIAVLITIGYLVKWIIAKDWK
                     HIGTAGALTIIAAIIGIAGNALVLKTTSEYSKFTMRGGKDISIEGDSVKAAKTSGLDT
                     SYAFEYSLGNAEALTLLMPDAFGGASSNMSSEESHIIDKLTSRGIPESDAQQLAQSLP
                     RYWGKLYTSGPAYLGVIIFLLGLIGFVIVKTPLRWGLLAATILGILMAWGKYLPGFNT
                     FLFEHLPLYNKFRSPAFAQVIPQFAMGVIAVLALHQILFSEKSKESLKNDFKKILYTA
                     GGLFGVLGILYIAMNYSSPIDGQIIAGYTDKNGSDEMARLIVSGLKEDRKAMFGGQIL
                     RALAFAILALGIIWLYAKDKLSSFVAAIVLLIISTLEITIVSKKYLSDESYISADDYA
                     NSNFAPSSIDQQILQDKDPNYRVFNMVGTSSGSTFSESKTSYFHKSVGGYHPAKLRIY
                     QDIIERYLSGRPSPMVLNMLNARYIVVQDPQNGQPGLITNPDAFGNCWLVKNVRLVEN
                     RVEAINALGTTNLKDTAIVEKSQLTNLAQPQLDSASSIKMTKFDNDAIEYEANCNGPQ
                     FAVFSEIYYPKGWNAYVDGKKVDYYNTNYILRGLPLPAGKHTVKFVFEPESVKQGRSI
                     MFIASILILVILLGGLFIAWKQSKKTS"
     gene            62266..>63366
                     /locus_tag="PLO70_01720"
     CDS             62266..>63366
                     /locus_tag="PLO70_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQZ73201.1"
                     /translation="MKKVLVIAPYPYLPYFSGGQKFIAQFLEYFANEVNLTVISVQEN
                     DFSLVKNYRTIPLLKKSFYRYIDLSLVKKISHEIKLNKYDAIIWEHPYYAWLAFSVKK
                     RTGIQTIIHTHNIEHQRFRSTGRWWWPLLKLYEKWSFKKADKLFFITPEDKDFAINSW
                     KTDPAKCIHLPFGITIKEHPSDNTSSKEAIRQQHAIAPDEKIILFNGLLRYKPNLDAL
                     KYILESINPLLLAAPSFRYKIIICGKDIPAELNALTSYTDKNIIFAGFVENIETYFKA
                     ADIFLNPVQSGGGIKTKMVEAIAYGATVIATESGAAGLDKKVCGNKLVTVPDNDWETF
                     AEKVLESSKTSTNTPAVYYETYYWGNIVKKIAS"
BASE COUNT        18933 a        13413 c        12058 g        18962 t
ORIGIN      
        1 accttacttt ccaaaattac tttccgtgga atactatcgc ctgcttcttt ggatgtaatc
       61 agttgtaata agatttgagt tgccttttcc gcctgttgat aagggaattg ctcaaccgat
      121 gcaacaggag gataatccat ataacttgtt ataggaagat tagcataact aacaaaagag
      181 atatccttat tgatcctgac tccctggcta cgggcatact ttatagcatc cagcgccaca
      241 taatcgttaa atgcaattac agcagtaggc ctgttcttta atgccagcaa tttatgcatc
      301 gcatcatttg tttttgtaac actcaggtca gttgccacca caaaagctgt atccggcttt
      361 attcgatgct tctttaaccc ctcctggtac ccaattaaac gttccctgga gtgaagaatc
      421 gcatcagggc ctttaataaa cccaattttt ttatgccctt gctgaaccag ccagtccacc
      481 aattcaaccg aactattttg caggttgcag gaaacagtgt atgcgtcagg attgccaggt
      541 accctatcga aaaaaacaac cggaatatta tatttttcta attgctcaaa atgctcatat
      601 gatttagtgg tcttagccag ggaaacaatc aacccatcta ccctgtggcg tcgcatggtg
      661 tcaacaatct gaacctctct ctccgtatta tcatgcgact gaccaatcag taccgtgtat
      721 ttattttgag tggcaacatc ttcaattcca ttgatcgcca tagaaaagaa ctcttctcca
      781 aggctgggca ggatgactcc cagggtcatt gtttttccct gtttgaacga aatagccgcc
      841 tggttaggtt cataatgaag ttcttctgct aatttctgaa cctgcatttt ggtacgaagc
      901 ccgatacttg gatgatcatg taatgccctt gacacggttg acaccgatac attcaaccgt
      961 tgtgctattt cctttattgt tgcgggcttt tgatccatgt aataaaaaag atgtcataaa
     1021 accgatacaa ggtataaaaa cgaatcgaat ttcgggctaa gattatacaa tcgaaaaaat
     1081 gctgtcagga aaatcaatta atcaaaataa ttttgggatt ggctgattct ttccgtaaat
     1141 ttcatgtaga aaaatacgcc ataatgaata atgtttcttc tgttcttacc cgaaaaaatt
     1201 ttcaacctgt tacagtttca cccgggacaa ctgtaataga agcattaaaa ataatggcag
     1261 aaaaaaatat tggttcttta gtaataatgg aaggtgataa ttttcagggc atcgtcacgg
     1321 aaagggacta ttcccgaaaa gtaatactga aaggaaaaaa ttccaccgaa actaaggtgt
     1381 cagagatcat gtcaaaagac cttccgataa ttaatccgtc agacaagatt gaacactgta
     1441 tggaacttat gacgatgaaa aacatccggt atatgccggt gtttgaaaat aataaactcg
     1501 ttggtatcat ttctatgagt gatgtggtga aggagactat cctggcccag aaagaaacca
     1561 tcaatcaatt acatagctac attaacagtt aacttacaca ctcattaaat acgcagcgaa
     1621 accccgtaag gtttcgcttt tttatttttc ttcttctttc ttttctttta ccactaccag
     1681 gtctttcatt tccacctgca tcggaagtaa cccgattttc acgaccgctc ttttaccccg
     1741 taattccatc acttcgccca cctggtgatt gcgtttcatc attaccttat taccgacttt
     1801 tatgtcacca ccaatctcac tgaactttgc atctatcttt ttctgcattt tgctcaccac
     1861 ttttttctca tctttcttaa acaatagcgc ctccatttgt tttattactt tatttttatt
     1921 ctcctccttt ctccaatcaa tcaataactg cttcagtctt cggtccatat ctttcaggta
     1981 agcaattttt tcttcggaaa tacgattttg ctctttcagc agttctacct gctgacggtg
     2041 tctttctttg tccatcacct gcaccatttc ttttttcagc cgttcatttt cctttatagc
     2101 ctggttcaga ttcatttcct ttttttcaat cgcctgtaag tcctgttcgg ttctgttcag
     2161 taatttatcc agcctgaagt ggtcttcatc aaccagctgt cttgcccggt ttattaaatt
     2221 tttatctaac ccaatcctct cagcaataga aaaagtataa gagctaccgg gtttgccgat
     2281 aagtaatttg tattgaggtt gcaagtgctt ctcatcaaag gccatagccc cgttaatgat
     2341 acccggagtt ttacctgcca tcactttcag gttcaggtaa tgcgttgtca ccacacccat
     2401 tgcatgcttt ttcactaact cctgtaatat tacttcggca aatgcaccac caaggttggg
     2461 gtcgctgcca ctgcccaatt catcaataaa gaacaaggtt ctgccattgg cattttccat
     2521 gaagtatttc atatgcaata aatggctgct gtaggtactt agttcaaatt cgatgctttg
     2581 cgtatcgcca atatgaatca tcagctgttt aaaaatacca aattgcgaag aggggtgtac
     2641 gggtaccaac aggccacttt gtaccatcat ctgtaaaaga ccaatagttt ttaacgtaac
     2701 agttttaccg ccagcattag gtccgctgat cactaaaatc cgttttgcgt catccaatgt
     2761 aatagaaacc ggaatcgtcg gcttattgac acgttgatta tacaaataca ataaaggatg
     2821 atacgcctca agtaaatgaa cggttgcttt atctgttacg gatggatatt ccccctttat
     2881 gtctcctgcc agctttgctt tggcccgtat aaaatcatat tcgccgatca cttcatggta
     2941 attggtaaga agggatgcat atacagagag cttagcggtt aattccctga ggatacggta
     3001 tacttctttc ctttcctcat tctccagttc atgtacctgg ttattcaatt caatggtctc
     3061 ttcaggttca acaaatgccg tcttcctgct gtcgctttct ccgtgcaaaa ttcctttaac
     3121 tgttcttttt tgctctgcaa aaacagcgac tacccgcctt ccactcataa aactttcctc
     3181 tatgtctgcg aggtacccct gtttattgag ctttgaaata attttttcaa acactttccg
     3241 cagctcattt cttttacggt ataaattcat ccggatttcc ttcaattcac ccgatgcatt
     3301 atccttgacc tgtccaaatt catccagcac ctcatcgatc aattcaagaa tcgctttctc
     3361 gtagtacgtc ccttcgataa ccctggtcag tgcatcatag gcaatccttc tttccgtatc
     3421 gaaccagcga aatatctttt caatactttc tgccagtttt ctgataagca ttaactgttc
     3481 tcctgttaat acggcacccg gtattgaaat cagcttcaac tctttggaca ggttcaatac
     3541 attgtcattg gggaagtaaa tactgttaac aatcaattgt ctgtactcat gactttgttt
     3601 cagatctact tcaatgaatt ctttgcgggt atgtatccga agttcccggg ccttattccg
     3661 ggcatgatcg gtctggcagt aattagccag caggtctttc actttgttaa actctaattg
     3721 tacaggtgcc gattcaggga aaagcttcat caaaatccgt ttaagtgggt atttcagggc
     3781 gctgcaaatt tcggactttc gatcatactt ctgatcctct ctgcatttta tgtgtgtata
     3841 caacagaaat aaaggcttgt tattaacgga tttcatccgt gaaaagggtc tcttatccct
     3901 gcttttatcc cattcataca attccttatc ttccgcctat aatatagaat tatacttgtg
     3961 tgtcttttat ctacaatcaa atcaatttta tgaagttaaa gtcattactt accgttctct
     4021 catcgggact aattttttcg gccatggcgc agaacaaacc cctgatgcaa acacaaaccg
     4081 gagctcccgg ggttaacctg gatacttcca agacaaaaaa gccagccggc attaccgaca
     4141 aagtaaaaag tagtaaaaag atagatggct tatttacttt ataccaggat acagccaccg
     4201 gaagtgtgca attgtatgtc aaaaaaaatc agctgggtaa agagtttatt taccaaagct
     4261 tttctattag cggacctacg cagttcttcc tgaatcaaag tatgcacagg gccacgtttg
     4321 tttttaaagt gcaaaaggca tttgacaaac ttgaattctc gagagtcaat acttcatttt
     4381 attatgacga aagcaatccg gtaagtaaga caaagaatgt ggataaacct gaagcagtat
     4441 tcttgtcaga aaaatatgta tcagaagatt ctgcaggata ccttattgca gctgatgcat
     4501 tttttatcag tgaaaaaatg gacccggtaa agccagccag ttttccaggc atgcctcccg
     4561 gtctttcttt tagtcttggc ggtttgaatc cccagaaatc aaaatatcat acgattcgtt
     4621 ctttcccgaa caacactgat gtggtagtag acatggccta tgataatccg ggagcgattg
     4681 tttcaggagg tccggacatt acagacccga gatatgtcag ggtacgtatg caacatagtt
     4741 taattgaaat gccttccaac gattttcgcc cccgtcgaga tgatccaaga gtgggctatt
     4801 tcatgcaaca ggtgactgac cagacaagta taagtcctgt tccatacaaa gacaagatca
     4861 atcgttggaa cttacagaaa aaagatccgg gtgcagctat cagtgagcct atagaaccaa
     4921 ttgtatattg ggtagagaat acaactcccc ttgaataccg tcaaacgata gttgatgccg
     4981 gtctaaaatg gaatgcagct tttgaaaaag ctggttttaa agatgcggta caaatgaaaa
     5041 taatgccgga cgatgctgat tgggatccgg cagatattcg ctataatgtt atacgctggg
     5101 tatcttctgc tcagccaggt tatggtgcca ttggaccaag ctttgtaaac cccaagacag
     5161 gtcagatatt gggtgcagat atcaccgtag aatggtttag cggaagtgct accccaatta
     5221 ttgacgaatt attcagcggg cctgctgctg acaatactaa acaggaattt aatatcccgg
     5281 gaatggacca tactcattat gcaacttgta ttatggccca ggaactgaaa gcacagctga
     5341 caaccggtat gacagtagca gatgccatgg atgccaaccc ggccgagatc aaagaaatgc
     5401 ataagcaatt cttactatac ctcattatgc atgaaatggg acacactctt ggcctgaacc
     5461 ataatatgaa agccagccag atgctttctc cctccgaagc acataataaa gagatcacca
     5521 gaaagattgg tcttataggc tctgttatgg actacccagc catcaatatt gcatccgata
     5581 aaagtaaaca aggtgattat tatactacag tagttgggcc ttatgatatc tgggctattg
     5641 aatacggata tactcctttt gccgaatcag cagaagaagc tgaactcaaa aagatcctca
     5701 gcaaaagcac agaccctaaa ctgacgtttg gtaatgatgg cgacgacatg cgggcaccgg
     5761 gtaaagctat ggatccaaga gtgaatgtga atgatatgag cagtgatgcg atcggatatg
     5821 ctgaagagag gttcaaactt gtgaataatg taatgggcag gttagtacag aaatacagca
     5881 aacccgggca aagctatgct gaacttcgtt cacgatatgg agcgttacag ggacaaagaa
     5941 atagtatgat tgctgctgtt agccgttaca ttggtggtgt ttatgttgac cgcagtaccc
     6001 ctgatcaaaa ttcaggtaac aaaccattta cgccggtacc attggctact caaaagaagg
     6061 caatggaagt attgagtaaa tatgtattgg cccctaatgc ttttgagagt gatgcacagg
     6121 tattccctta cctgcaacct caacgccgtg gttttaatca aaatggaaat ggtgatgatt
     6181 atagaatcac cagcgtaata ctgaataacc agggagccgc gattgcacat atattacatc
     6241 catccacggt acagcgtatt accaacagcc gtctctatgg caaccaatac agcgttgctg
     6301 atgttatgaa tgacctgtta aaggcctgtt tcgatgcaga catgaagggt aatgtaaacg
     6361 tttatcgtca atacctgcag actgcatttg taaaacaagc agccataata ctggatccca
     6421 aatctcccgg atatgatgac gtagcaaggg ctgctgcctt gaataccgtt aagaaattaa
     6481 aaacacaatt agcctcagcc gtttcgctga atgaagaaac aaaagcacac agaggaagcc
     6541 tgttatttct gatcaataat gcactggagc gaaaataacc aggttaagaa taaataaaaa
     6601 acccatcgtt cggacgatgg gttttttatt taataatacc acatcgctga tatcatcaag
     6661 gagcctgggt tataattcat tagtagaatt atcattcttc tattataaac ttattaatat
     6721 tagcgtaaaa caaagatgtt aaaataatta caagactctt cataataaat cctctttatt
     6781 caactcttcg taagttaatt tgttatatac aaacgtagaa cctgcaattt aagcagttgc
     6841 caaaagaata attgtccgaa attcatataa tgattcctaa aactgatata atgaaacctt
     6901 ttttaacggt ggctatagca ggactcgcaa ccttgtcttc ctgtgcccag caatctacag
     6961 caacagataa aataaaaaaa gataataatt atatgacaac ccaacccaat acagccatag
     7021 ctacttttgc caacggatgt ttctggtgta ctgaagccat ttttgaagaa ctggacggtg
     7081 tgattagtgc agtgtccgga tacacaggtg gacaaacacc caatcccact tataaagaag
     7141 tcagcaatgg ggagaccggg catgcagaat gccttcaaat aacatatgat accacaaaaa
     7201 taacatttga tgaactgctg gaagttttct ggcaaacaca tgatcccact acactcaacc
     7261 ggcagggagc agatgttggc acgcaatacc gaagcggcat tttttatcac aattcagaac
     7321 aaaaagaaaa agcggaaaaa tataaaacgg aactggataa aagtggggct tttaacaacc
     7381 ctattgtaac agagataaca gctttcagca aattttatcc tgcagaggac tatcaccagc
     7441 agtattatga attaaatggc aacagcaatc cttattgtaa gatagtgata cagcctaagc
     7501 tggaaaaatt cagaaaagtt tttaagaaca aattgaaagt cgataattga tagataaata
     7561 aagcggggat aacccgcttt attttattga actattattt tctgactatt aaactcctgc
     7621 gctgttcgaa ccacaagtac aaaataaccc ctccctgctt tgaaataggt caaatcaaca
     7681 acattaactc ccttcaagag tttagttttt ttactatata ccatcctccc ggccatatct
     7741 gccacttcca actgtacttc tttctggtca ttacattgta ttgtaactgc aggatcagcc
     7801 tgacgcaaaa tagtattata aaacgtaaac atttttttct cattacccac ttttatcata
     7861 attgtattac ttatttcata gattttttga atagtattta caagtttgat cctgtaatag
     7921 ttaactcctt cataaggatg actatccaaa aaagtaaatg gaaattcgcc ggatccacta
     7981 ttggttattg ccaaatttcc agcctcttca aaaaaagatc cgttactact tttctcaata
     8041 ataattttat catatgtatg actggcagat aaaacacctt ttaactcaac ctggtttgat
     8101 ttgaatactc cattaaaact tattaatgtc ctgttgggca atactatttc aaaaggatag
     8161 gaaaatcttt gaaaatacaa acttcgtaat cttgatgcgc cagtatttga ttgattatct
     8221 gcccctaccc gtatactgat tgagctgatg tttgcattaa tagttgtaaa cattactgtt
     8281 ttttgtgctg tatcaattcc gggataatcc actgctgctt tatttttacc cctagcccag
     8341 gttccattgc gggacatgtt taattcactt ccggcccact gaaaataagt atacccgttt
     8401 ggcatatcta cttcatcaaa ttcgtaaaga ggaagaccag agtacatttg accatctacg
     8461 tcaatcgggg taaccggaac ctctgtttgt ataagagaat tatttgtgcc agcctcaaaa
     8521 aaatcaattt taaattcaat ataaccattt cgatttgccg gaacagatat tacaggctgt
     8581 aatgccttgt caaaacctcc ccctccatca atatttgtta ttgaaattcc tcctgtaata
     8641 tctgctatag taacagttgc atcaactcct gcttttactg aagaaaactt atacactgca
     8701 ccaatactta atgcagtacc agaaattaat gtagcccctt taaattcata ttttgattgg
     8761 gcaattgcga aagatggaag caaaaaaata gaaatgctaa atagcattaa aatgcgaata
     8821 gaaaatagtt tcatagtacg gttttttagt ttttaccagg gttgtgatta tcggctcaac
     8881 agaggaactt ggaattgctt tcaaaaacta cgatacacta tatgaaaatg tacccggctt
     8941 tttacttacg gttcaaaagt aatatacgat gtggaattat gcaatagtcc acgtataaat
     9001 cctatagtga aacagctcgt ataattacga tttaaagcga aaaagagttg aaaaaaaata
     9061 ttattttatt tctgtgcgcc ttaactcaag caggttaagt gcgttgggat taaatcctgt
     9121 tacattgggc tgaaccaaat gaacggcttt gtcataccat aaaggtacca ccggtgcgtc
     9181 ctgtatcatc acctggtccg cctgctggta aagcttatag cgcaacgaat catttgtttc
     9241 tgcgatggcc ttttcgaaaa gaacatcaaa aaccgggttt ttataccggg tataattagg
     9301 cggtgcgggg tttttggaat agaaaacaga gaggtaattt tcagcatcag gataatccgc
     9361 tatccagctt ccccggaaaa atgcagctct tgaattagat gtcatgtcca gcagcaaaga
     9421 tttctgtaca acttctacct gtaccggtat accagattcc tccagttgtt tggcaataaa
     9481 attcgccatg tcagcataaa tagcaatggt cagcagtttt atggcattca tcccctttcc
     9541 atccgggtat cctgcttctg ctaataactg ttttgttttc gccgggttat aattgtatcc
     9601 ttttactaca gatgaatcaa aagatggcaa acccataggt acaaaaccag attccgcagg
     9661 cgtacccaat gaattgcgta gatacaggat cattttacgc ctgtcgaatc caaagttaat
     9721 agcctgccgg atttttcttg acttcattgg tgagtccttc aacagttcat tacttgaatc
     9781 aaccaaaatt cccaggtatt ctatattgag atatgggctg gtatgcaatg cgatctttcc
     9841 ttcccaattt ttacgcagtg taccattctt tgtaagtact tcatctttaa aggaagcttc
     9901 gatatcattg atgaactcaa gctctttctg acggaacaat aagaattcag ttgccttact
     9961 atcataaaaa ctaactttaa tcccgtcaag ataaggcaac tggttaccgg cactgtctct
    10021 ttcaaaataa ttttcgtttt tctttagcac caaagcctgg ccttcttccc agtgtacaaa
    10081 acggaatggc ccggtgccaa cagggtgacg acgaaaatct gtgccataca tttccactac
    10141 ttcctttggt acaacagaac aatattgcat ggaaagaata cccagaatcg gattatacgg
    10201 acgcagcagt tttaattgaa aagtagaatc atcaatggct ttaaatggct ggattgtatc
    10261 tgctttcctg ttaaatatcc aggaaccggg actggccacc tgcttatcaa tgatcctttt
    10321 gagactatac tcaatatcag cggctgtcat ctttctgccc tttccctttt caaatgcagc
    10381 gtcatcatga aaaaagacgt cagttctgag atgaaaagta tagatagtac gatcaggtga
    10441 aatatcccag cttttggcca gggaaggaac aatatggaga ctgtcatcta tttcaaccag
    10501 tgtattgaac aactggtgag caggccacat ggtagcctgg cttttggcaa aagccggatc
    10561 caatgtgggg atacccgaaa attcattata atggaagatc ttgccttcat gactcttttt
    10621 attataacaa gaggtcaaca acagcaacat tatccaaaaa agcagagaat attttgttaa
    10681 acggttcatg aattaattac aaccggtcta aattagcaaa gtatcggaaa cattcattca
    10741 atcaaattaa gatggctacg ctattttttc gttttagttt cattgtacct ctcttttttg
    10801 taatgtatag ttgtgcacag ccattaaacc ggcaaaatca atttacacgt caggatacac
    10861 taagaggcag tataacgcca gaacgggcct ggtgggatgt tgcttcctat aatatacagg
    10921 taactcctga ctataaaacc cggacaataa gcggctggaa ccagatcagt tttgacatca
    10981 acacagatgg caaaaacaaa aagatgcaaa ttgacctgca acaaccgatg gagattgaca
    11041 gtatcctatt tgagaaaaaa aagatcagca catttaaacg gttgggtaat gtatatatca
    11101 ttgattttgg caattatgaa ttcatagcag ctaggacatt ggtaaaaaaa gagaaagcca
    11161 taccccttca cagtataaaa atttattttc atggcaaacc ccgggaagcc atcaatccgc
    11221 cctggaatgg aggctggata tggagtaaag atgccaaagg acgtccctgg atgactgtcg
    11281 cctgccaggg attaggagcc agcgtttggt atccctgtaa ggattaccag ggtgatgaac
    11341 cagatctcgg tgcgctttta caggtacgag ttccggatac actggttgca gttgccaatg
    11401 gaaaattaca tgctgctgat gatcagaaag atggtactgt tttatacacc tggcaggtaa
    11461 aaaatccgat caacaactat aatatcgttc cttatatcgg taaatatgta ctctggtcgg
    11521 ataattatat tggcgagact gaaaaaaatc tgtcactaga ctactgggtt ttagattata
    11581 atttagaaaa gtcaaaaaaa cagtttggcc gggatgtaaa aagaatgctg gaagcatttg
    11641 aatattggtt tggtccctat cctttttatg aagatggatt taaaattgta gagtcctccc
    11701 atttgggaat ggaacaccag agtgctatcg cttatggcaa ttattacctg gatggttatc
    11761 tcggtatgga tctttccggc agcggatggg gcaaaaaatg ggattatatt attgttcatg
    11821 aaagtggcca tgaatggttt gccaataata tcacaacaaa tgatattgca gatatgtggg
    11881 tacatgaagg atttacgatg tactcggaag tattgtttac agaataccac tatggtaaac
    11941 aggccgggga tgaatacctg cagggcatcc gtcaaaaaat cagcaatgac aggccactga
    12001 tcggccctta tggggtgaat aaagaaggaa gcggtgacat gtataataaa ggagccagcc
    12061 tgctgcatac aattcggcag gttatcaatg atgatactgt tttcagaaaa atactcaggg
    12121 gactgaataa agatttctat cacaagatgg tagatacaaa agatgtggaa gactattttt
    12181 ccaggtattc gggtaaagat ttgtcaaaaa tctttgacca gttcctgcga acaactgaaa
    12241 ttccggttct ggaatataaa attaatgccg gtaatttttc ctaccgttgg gcaaattgca
    12301 tcagcggttt taatatgccg gtttacacag aaaagatcgg ctggctttcc ccgacaactg
    12361 aatggaaaga caaaatgatc ccggctggcc tgattgaaca attaaatgca aatgccaatt
    12421 tctacgtcac tactaaaaag gtaaactgag tacacccggt caataacata ttggtcattg
    12481 atctttcaca acttaccttt accacatgag taatatccgc cggcaaagca ttatttcatc
    12541 tctggttatt tatgttggat ttgctgttgg attaatcaac gtttattttt ttaccaagca
    12601 gggtcttttc acagaagccc agtatggcct cactaccgtt ttcattgcca tagccactat
    12661 gatgagcagt ctttcgaata tggcaatgcc ctcttatatc tacaaatttt atccttatta
    12721 taaagaccat ttgccggacc ggaaaaatga catgctgtcg tgggcactgc ttaccggctt
    12781 tattggtttt atccttgtaa tggcagccgg atggtttttg aaagaccttg tcattcaaaa
    12841 atatggcacc aattcaccat tgctggtggt ttactattat tggattttcc cgcttggact
    12901 aggccttacc atctattcaa tccttgaagc ctatacctgg agcattggta aaccagtcgt
    12961 taccaactat tttaaagagg tacaatggag attatttaca actatattaa tactcctctt
    13021 tcttctgaat gtcatcagcg atttcgactt attcataaaa ctctttgcat ttacttaccc
    13081 gggcatcgcc atcggacttt tcatttacct gcttatcacc aaaaaaattc atttcgcttt
    13141 tactgtcagt aaggtcagca ggagattcct gaaaaaaata attctgttct gctctttctt
    13201 ttatggtgcg atgctgatac aaaccctttc aaaggttttt gattcttttg ttattgcttc
    13261 agtactggac gatggcctgg ataaagccgg aatttttgca ttggccactg tcgttacaag
    13321 tatcatccag gctccgcaaa gaggtattgt atctgccgct atgcctcatt tatcgagggc
    13381 atggaaagaa aagaatacag cgttgctgca aaaggtttac cagcggtctt ccataaatca
    13441 acttctcttt gcctgtgcaa tcttcctgct aatctggctc aactttactg atgggataat
    13501 tacatttgga ttaaaaccca tttacctgga tgctgcctgg gtcttctttg ttttaggtat
    13561 taccgttata atcgatatgg gcaccggggt taattcacag atcattgtca catcgactta
    13621 ctggaaattt gaacttatca gtggtatagt gctcttactc atcatgctgc cccttaccta
    13681 cctgcttaca aaacagtatg gtatcatcgg tccggcattt gccaccctgg tttcaatgag
    13741 tgtgtataac cttatgcgga tcattttttt atggaaaaaa ttcaggttat ttccgctaac
    13801 agttcagtca ggttatacat tgctacttgc aactgtatgc tatggtattt gctattttgc
    13861 cttcaggaat atcgatggcc tggcagctat ttttttaagg agtatcgcat tcataacctt
    13921 atacggagca ggagcaatct atatgagatt atcaccggat attcaaccgg tgcttcaaaa
    13981 tgtccagaaa agattaggaa taagaaagta attatcagtt gctggctaca gcttgcttgt
    14041 catccattct acctgcacca acaaaccgtt tcaggtagta tggttcaaat aaatcattta
    14101 ccataactcc ttttgaaaca gaagcatgaa tgaatttgtt attacccagg tataccccca
    14161 catgagatac gccacggcca ttggtattaa aaaaaacaag atcaccttcc tgtaattctg
    14221 tagtagatat tttcctgctg atgcggtatt gctcacggga aactcttgga atcatcatac
    14281 catacacggc agcataaact gctacggtta gtcctgaaca atcaataccc gtttttgtat
    14341 ttccacctac acggtatctt acaccgtacc actcgtccac actttcaagt aaagatctgc
    14401 ttggcaatga ttctacttca gtattcagaa gtattgaata ttttaattgg acagaagaag
    14461 cactctctat ggatggtgta cgattagcaa acaggtttac tgatgactgg ggttcctccc
    14521 tggtaactgt ctcctttaat gctttcctgt tatcttccac cttaataacc tcattctgga
    14581 tttttacagc cttcgttttt ttaatttccc caatctcctg ttgtgccgtt ttaacttcag
    14641 ccatctgggg agtaacagaa atttcttcaa taaattttgc gttggaagga gctactgatt
    14701 cctcggatgc agaaagttgc ttattactgg taaaattcaa cggcttgaat gtagaacagc
    14761 tgctgagcag tattataaat gctaatgctg gtaaaaattg cttaaccata cgggttatca
    14821 tgttaagttg atttcatagg tcggttaaaa acctgttttc ctggatttgc tgattccccg
    14881 tagggaggcg tgcaaaaata taggttatta tcctaaaaaa ttgacatccc ttaaacgaat
    14941 tttccgggta aaaattgcca catccctaaa cccctgtaag taaatgattt atagtttcta
    15001 acaggctgtg tatattgagt aaaatcaacc caattgccag gtgcgattat ttgattatta
    15061 ttgaagcaac tattaaacca tgcaagctct attctcctac ttcactttgc ttttgttaac
    15121 agcgggttgc caaaataccg cggatgataa aggttacacg aaattactgg cagccagtgt
    15181 agttgtcaga gataccattg tccctttact tccaccaccc cccaggcctt attccgaact
    15241 tgaatttgaa aaaaaggaag tgctggcagc cgcctccggg acaaaatcag tccttttcaa
    15301 ttcaaaaggg tcaaagttat ttgcgatgaa cctggaaggc atgagtgttt acgaatttga
    15361 ccgggcctcc agaaaaataa cccgtgaatt taagttcaaa cccaccaggg gaacaggatg
    15421 ggattacaat aaaagcaggc ccattgcatc ttaccaggaa aaaccggttg aagcctgttt
    15481 tagccatgat gatagaattt tatgggtttc cttgcacaat gccgatggga ttattccaat
    15541 ctgggtagat gatttttcaa aaaataatgc aaagcgttct gcaaaccaaa aaacaaaacc
    15601 ggtaactgtc atttaccccg gcagcaataa aaaagattca ttccaggtgc ctttgattga
    15661 aacaggaaaa acaccaaaag taatctcaag aacagctgac aataaaaatc tgctcgtcag
    15721 caactggcat tcccgtaatg tgagtgtgtt ggaaatgaat gatacgataa caccctacgg
    15781 aaaagtaatc agtaccattc ctgtttcagc cataccaaga ggtattgcag tagacgataa
    15841 aaccggtctg tcttatgtgg ctatcatggg cggatcaagt attacagtca tcagcaattc
    15901 ggtctggatg aaagaagacg atatgaatgt ggcagccagt ccccggcacc tggtaatgga
    15961 taccagcgga catttatttg tctcttacaa cagtcttggc actattgcct gcctggatgc
    16021 cgccacgggc aaatctttat tcactgccaa aacccatgca cagcccagga caatcgtact
    16081 gtcaaaaaat tacaaattca tttttgtaac ctgctacagc agtgattttg tggatgtgta
    16141 caaaatcaat gctgacaact ttgagaaagt ggcatctttg ccatgtagtg gtcacccggt
    16201 aggtgtggat atttttgaga atgatgacaa actggaagca tgggtgtgca gctacagtaa
    16261 cggcctgatt aatatttata gttttaaaaa gaaataaagg acagtgagta tgaaattttc
    16321 gcatctccac gtacacacac aattctcctt attagacgga gccgcttcta ttcagaatct
    16381 ttataagaaa gccattaaag acggcatgcc tgcccttgcc attagtgacc atggcaatat
    16441 gtttggtgcc tttgaatttg tagccgaagc gtataaacat aaagacgaga acggcaatgt
    16501 aaaagtaaag cccatcgtag gatgtgaatt ttacattacg gaaacaagac accaaaagac
    16561 ttttaccaag gaacaaaaag acaaacgcta tcaccagata ctgcttgcaa aaaatgaaca
    16621 ggggtacagg aatcttgtaa aacttacttc gcttggtttt attgaaggca tgtatggcaa
    16681 atacccccgc attgataaag agcttatcct gaaatatcat gagggattga ttgccaccac
    16741 ctgctgcctc ggggcatcgg tgccacaggc aatactcaga aaaggggatg aagatgggga
    16801 aaatgaattt aaatggtggc tgaatgtatt ccaggaggat tattatgtgg aattgcagcg
    16861 tcatggcatg gccgaacagg ataaagtaaa tgatgtattg ataaagtttg ctaaaaagta
    16921 taatgtaaaa atcatagcca gcaatgactc tcactatgtg gagcaggatg attttaatgc
    16981 acatgatatt ttactttgta tcaataccgg ggaaaaacag gctactcctg ccctgcggga
    17041 ttttgcagat gatgatgtac atataaaaga taaacgcttt gctttcccga acgaccagtt
    17101 ctatttcaag accacagaag aaatgagcaa ggtctttcac gacctgcccg aagcaattga
    17161 taacaccaac gaggttgttg ataaaataga gttactgaat cttacccgtg atattctatt
    17221 acccaacttc cccgttgaga aaagatttga gatacacagt aaagaagaaa caatcggcaa
    17281 atacaaagta tcagctgaat cgcagaatca atgggaatat ctgaaacata tcacttacga
    17341 aggtgccgca aaaaggtata ctgaattaac tgatgatgta aaagaaagga ttgaattcga
    17401 acttttcact atcaaaatga tgggctttgc cggttatttc ctgattgtaa gtgattttat
    17461 aaaagccggc agagacaaag gagtgtttat tggtcccggc cggggttctg ctgccggttc
    17521 tgtagtggcc tattgtatcg gcattaccaa tatagacccc atcaaataca atttactatt
    17581 tgagaggttc ctgaatccgg accgtaaatc aatgcccgat atagatactg actttgatga
    17641 tgaagggaga caaaaggtaa ttgattacgt tgttgaaaaa tacggcaaga accaggtagc
    17701 acagatcatt acctatggca ccatggctgc caaatcaagt attgcggatg tagcaagagt
    17761 gatggacctg cccttaccgg aaagcagggc cttaacaaaa ttagtcccgg atcgtcccgg
    17821 aacagaattg aaaagggtgc tgcatgcacc ccttaaagcg aaggatggtg aaaaatcgct
    17881 ggaagaaaaa gatggcctgg gtaatgatga cctggaaaat gtaaaaaaac tcagagagat
    17941 ttataaccag tcaggttcgc cggccagtaa agtattacat gaagcagagc ggctggaagg
    18001 atcggtacgt aacaccggta tccatgcggc aggaatcatt attgcgccaa gagatctcac
    18061 ggaattaatt ccagttgcca cttctaaaga gtcaacctta tggctgacac agatcgaagg
    18121 aacagatatt gagaatgcag gtgttattaa aatggacttc cttggattaa aaacattgac
    18181 cattattaaa aatgctttag cacttatcaa attgaatcat ggcattgaaa taaatattga
    18241 tgaaattccg ctggatgata aaaaaacatt tgaattatat cagcacggtt ctacaatcgg
    18301 cacattccag tttgaaagtg ccggtatgca gaaatacctg agagacctga aacctgataa
    18361 attcggcgac ctcattgcca tgaatgccct gtatcgcccc gggcccatag catatatccc
    18421 caattacatt gaccgtaaac atggtaggga agctatctct tacgatgttc cggaaatggc
    18481 agaatacctg gaagaaacct atggcattac agtataccag gaacaggtaa tgctgctggc
    18541 tcaaaaatta gcaggtttca gcaaagggga tgcagacgtt ttgcgtaaag cgatgggtaa
    18601 aaaacaaaaa gcggtgctgg ataaaatgaa agcacagttc attgcaggag ctactgaaaa
    18661 attgctgcct ccggaaaagc tggaaaaaat atggacggac tgggaggcat ttgcccaata
    18721 tgcattcaac aaatctcact ctacctgtta cgcatatgta gcgtaccaaa cagcttattt
    18781 aaaggcccac tatcctgctg agtatatgtc agcggttttg aacaacgccg gcagtattga
    18841 aaagatcacc ttcttcatgg aagagtgtaa acgaatggga ataaaagtac tggggcctga
    18901 tatcaacgaa tcgctaaaag gatttgcagt aaacgataag ggtgaaatac gttttggcct
    18961 gggcggattg aaaggtgtag gtgaagcggc cgttgaaagt attattgaag aaaggaaaaa
    19021 gggcggcacc ttcaccaata tttttgactt tatcaggcgc atcaaccagc gaacagtaaa
    19081 taaaaagaca ttggaaagcc ttgcctatgc aggaggcttt gattgttttc ctgaacatca
    19141 ccgggcacag tatttcacta tccctgaagg ggaaacagtt aacggcctgg agagggtgat
    19201 caaatacgga caggtgatgg cttcacaaaa tgccacaacc accaatacgt tgtttggtga
    19261 cctgcccatc agcatggaaa tacctccgcc acggattact gattgtgcac cctggccatt
    19321 gattgtacaa ctggatcatg aaaaagaagt taccggaata ttcctgagcg gccatccgtt
    19381 ggataattac aaatttgaaa tgaagcacta tggtgtaact ccattggctg atttcaatga
    19441 gttcaaagaa gcgataaaaa tgcagccaaa tccgggcaga ccgttccgta ttgtggggct
    19501 ggtatcagat gcacagcacc ggatagccag aagtggtaac aagtatggca attttgtgat
    19561 cgaagactat tctggcaaaa ctgaatttcc cctgttcagt gaagattata tgcggctgac
    19621 tcccatattg caacagggca gtactgttct tatacacgga tatttcaaac aacgatacaa
    19681 taaagatgaa tttgaattta aggttatgag tgtctcgctg gctgaaacca tgaagcgaaa
    19741 catgacaaaa caacttacca ttgaggcaca tccgcaggat atcagtagcg aattggtaaa
    19801 ctttattgaa aaaaatgtta agaataatcc cggcaagtca acccttaaat tcactttatc
    19861 agaacctaag aacaagatga aaatcagcct ggtgacaatg aatagcggtt ttgagatgaa
    19921 cgaagaaatg atccagtttt tggaaaataa accagaactg gaggtgcagg ttgtaactag
    19981 ctgatttacg aaggagaaag aggctttacc gttttcaacg cctaagcctg tcaatttttc
    20041 cattagttgg acaagttttg cacaactttg tgccagtatc gcatgttatt gaggcaggac
    20101 ttaaatttgc aatcccttta aaaacaaaaa ataaaattta tacaaatggc aaaagaattc
    20161 acagactcta atttccagtc ggatgtactg gcttctgata aactgaccgt gatagatttc
    20221 tgggcggaat ggtgtggtcc ctgccgtgca atcgggcccg ttattgaaga attatcaaaa
    20281 gaatatgatg gaaaggccaa catcggtaaa ctgaatgttg acaataaccc tgaggtatcc
    20341 atgaactatg gcatcacttc catacctgct atcctgttta tcaaaaacgg gcaggtagtt
    20401 gataaattag taggtgcgca gcctaaagca aattttgtaa agaaaattga ggcgcatatt
    20461 taagcttatt ccttataaaa taaaaacccc gttcttctga acggggtttt ttataccagt
    20521 tctgctcaat ttaatgagtc actttttcta ttacatcagg attatgctta tgcttgaaga
    20581 gcactgcaaa taacaccgca acaacgagtg catatgctgc aaacgccagc cagatattct
    20641 gccagtcctt atctgctcca ttcacagtaa aatatttgtc aataacataa ccacttattt
    20701 tactacccaa aaaagctcct accccatttg tcatcatcat aaacaaaccc tgggcactgg
    20761 accggataga agaatcggta gtggtttcaa caaataatga acccgatata ttaaagaagt
    20821 cgaatgccat tccatagacg atacaggaaa gaatgatcat ccacaatcca tcggctggat
    20881 taccataagc aaacagacca aaccgcagca cccaggcaat catagaaaag agcattacct
    20941 ttttaattcc gaactttttc aggaaaaacg ggatagtaag aataaataca gtttccgata
    21001 tctgggaaat agacatgatg atagtggaat attttaccac aaaagactct ggcccgtatt
    21061 ccggtacttt tctgaaatca tccaggaatg tatctccata catattggta agctgtaagg
    21121 cagcgccaag aaacatagag aagataaaaa acaacgccat cttgtatttt gcaaagagcc
    21181 tgaaagcatt caaacctaat tgctcaataa tggatgcatc tttagcaata gatcgttgcg
    21241 ggggacattt gggcaaggtg aaggaataaa tcccaagcaa aatagccgct acccctccaa
    21301 ttataaactg gttggcattt gctttacttc cggtgaggtt tgtcatccac atagctacaa
    21361 taaaaccaat tgttccccag accctgatcg gtggaaaaac cttcaccaca tcgtagttat
    21421 tttgtttgag taaggtgtaa gaaatagaat tagataaaga gatggtaggc atataaaaga
    21481 gcatagccac caggatgaca taataaagag taccggcatc tttaacctgt ggtacataca
    21541 gcagaaccgc tccatataaa atatgcagga gtccatataa cctttcggca ttcatccact
    21601 tatcagcaat tatacctgta agggcaggca tgatcagaga agatatggcc agcgtcgaaa
    21661 agattgcccc aaactgggct cctgtgccca tattattaga gaaaaaataa gtggcaattg
    21721 taataagcca ggcaccccac acaaagaatt gcagaaaact caatacagtc agccttcctt
    21781 ttaaattcat aaaaatgaaa ttattgaaaa gggaagataa ggattttagt gttttgaaag
    21841 acaaacatac cagtacagga aggcactaag tattccatat aatgtacccc ttttctttcc
    21901 agctgtttct caaactctct gtccctgcct ttccttagtt accttttgta gttgtaactt
    21961 tgcccgcagt taattgttac agtaatatga tcaccacaac agcaatttcc cccgagcttc
    22021 agtccatagc tgaaaaagta aaagcaggcc agcgaataaa cgatgaggat tgtttactac
    22081 tatttgaaaa agcaagtctt ggttttgcag gcagcctggc caatgccatc aaagaaaaac
    22141 tgcatggcga taaagttttt ttcaaccgta attttcatat tgagccaaca aatgtctgtg
    22201 tcttcagttg caacttctgc agttatagta aactctatgc acaccgggaa gaaggctggg
    22261 aattgagtat tgagcagatg ttgcacatgg tgaaaaaata tgacgggaaa cctataacag
    22321 aagtacatat tgtaggaggt gtgcacccga agatggacct gcagttcttt gcagatctgc
    22381 taaaggccat taaaacacat cgccctgaat tgcatattaa agcatttacg gctgtggaat
    22441 acgattatat gttccgcaaa gcaaaagtga gtgcagaaga gggtttgaaa tttttgattg
    22501 cagccgggct tgattctata cctggcgggg gtgctgaaat ttttcacccg gagataagag
    22561 aaaagatatg tgccgataaa gtggatgcgg atggctggct gaatattcat gaaacagcac
    22621 ataaactagg attacattcc aatgcgacga tgctttacgg gcatattgaa aagtttgaac
    22681 accgcattga tcatatgaga agattaagag agttgcagga caaaaccaat ggcttcaaca
    22741 ctttcatccc attgaaattc cgcaataagg ataatgatat gagcaatgtg ccggaaagca
    22801 cccatattga agacatgaag atgtatgcta ttgcccggat ctatctcgac aatttccctc
    22861 acctgaaagc ttactggccg atgatcggca gacagaatgc ccaactctct ctttcatttg
    22921 gtgtagatga tattgacgga acaattgacg acagcactaa aatatacagc atggcaggat
    22981 ctgaagaaca aagtccatcc atgacaacag ctgaactggt aacattgatc aggcaggcca
    23041 agagagaacc cgtggagaga gataccctgt ataatgaaat caaaaactat agtaacgttg
    23101 ctgcggaagt aaatgccggg ttgaattaat ttaattattc ctgttttatg aatcaaccat
    23161 ttcaattact ttaataataa attcataaaa aaagtaaaaa agtaaagccg cctgaaatca
    23221 ggcggcttta cttttatgag ttgagaaatt ttatactccc ggttcatctt cttccttttc
    23281 atcatcagct tctgctatat catcctcctc aaaaccggtc cctactacgt catcatcatc
    23341 aactgtaatg attgtctcgg ttacttccag ttccccatcc ggtgttatgt atatatcttt
    23401 tacttcttcc accataacag gaatcacact tacgggagct tttgtttccg gttgtgctac
    23461 tttaacggtc ctggccggac ttttcttttt caccactttt gcttttacag caactgcttt
    23521 tttaggcatt gcttttttag gcactgcttt tttgacaact ttctttacca cttttttctt
    23581 agctgctttc gctttctttg gagctacttt tttctttgga gctgccttac gctttggttg
    23641 ggctttagct actttctttt tcaccacctt cttcaccact ttcttcttaa ctgctttcac
    23701 tctctttggt gctgcttttt tctttgttac tttctttttt gcagttgctt tccttttggg
    23761 agttggttta gcaggttttt tctttgcttt ctttttagta gccatgacag aataatttaa
    23821 atgttaagga taatcatgtt gtcttacggg gacactaaaa ttacaacttg ctttgactaa
    23881 aagcttaatg tgcagaaaaa attacaggta aatagctaac actccggcag actgaggggt
    23941 acattcactg ccagcccgcc atcagccgtt tccttatact tatggttcat gtcaagggct
    24001 gtctcccaca tcgtttttat aacagcatca agggatactt tagcaaaatc aggggtgctc
    24061 tgcagggcca actgggaagc cgtgatagct ttaattgccc ccatagtatt tcgctcaatg
    24121 caaggcacct gcaccaggcc ggcaatcgga tcacaggtca ggcccaggtg atgctccatg
    24181 gcaatttccg aagccatcat cacttgtttt tgtgttccac ccatacattc agtaagaccg
    24241 gcagccgcca tggcagaaga aaccccgatt tctgcctggc atcctcccat cgcagcagaa
    24301 agagtggctc cttttttaaa gatgcttcca atttccgcag ctgttaataa aaattgtatg
    24361 atcttatctt cattatttcc attgcaaaat gaaatataat attgcaggac tgcaggtatt
    24421 acacccgcag cgccattggt gggtgccgtt accacccggc caaaggaggc attctcttca
    24481 ttcacggcaa gcgcaaagca actgacccag tccaatgtgt atttaaaatc attcccaccg
    24541 gaacggattg ccatcagcca tgaatcataa tcgtggtatt ctttacctga cagcagcttt
    24601 ttattgagcg ctgcactgcg acgttttaca ttcagcccgc ccggcaatat accgggagta
    24661 tgacaacccc ggtagataca ttctttcatt acccgccaga tattcaaagc actatttctt
    24721 gtttcctctt caccccgcca ggccgtttca ttttccatca caatttcatg aatgctcagg
    24781 cctgttttaa tacaccagtg cagcaggtca tcagcatcat ttaccggaaa tggtaattga
    24841 acagtttgta ctgttccggc ctcttctcct tcttttataa cgaaaccgcc gccaacggag
    24901 taatatgttt cggcaattga ttcaccattt cgcatcgttg ccagaaaagt aacagcatta
    24961 ggatgatacg gcaaggtttc tgtgacaagg aactctatat cttcctgcgg atcaaaatcc
    25021 acttcatgca taccgagcaa caccattttt ttcatggcag ctatatcacg gatttttgaa
    25081 tcgatctgat ctacagcaaa tgtaaccgga tcatcaccac ataatcccag ttgcacagcc
    25141 acatccgtac catggccctt gccagtttta gctaatgagc catacaacag cactcgtacc
    25201 tgtgttgttt cagttaacct attttcctgc attaatgatt gaacgaagcg ctgggctgcc
    25261 ctccaggggc ccaatgtatg agaactggaa ggacctaccc cgattttaaa aatatcaaag
    25321 acagaaattg cttcgtatgg caaaatgtaa tgtttaaaat caaaaataca gtaaaaaacc
    25381 ccgtcactgc aagaagcagc aggacggggc agcagagcat aagcagtaag gctcagctat
    25441 tattgaacat cgacaagttc tatttcaaaa tataagatgg catcggaagg aatagaccca
    25501 taacccgtac aaccatatgc cagcgatgag gggaggatta attttatcgt tccgccttcc
    25561 tgtatcaatg gcatccctat ttgccatccc tgtatcacct gggtaagcgg aaaagtgata
    25621 ggtgttccgg tctggctatc gaatattgtt ccactcagca gtttccccgt atatcttacg
    25681 gatacggttg aattaatagt gggtgctggt ccggagcctg catttatcac ctggtaatgt
    25741 aaaccactgc tatgagcagt agtcgtcata ccattggcca gcgcataatt aacaatagct
    25801 gcctgctcac tctcgacagt tttggcattg caactatcat ctttcaggca accgctgttc
    25861 aacaataaaa tgaaggatac ggacaaaaac agagctattt ttttcatata tgattattgg
    25921 tacaaaatct gttgtaagac cccgtaaaac tataattcgc tgcaacaggg ggtaaaattt
    25981 taagattcct ctttcctgct taacaattca cggtagcgct gttcattcat atcccagcgg
    26041 tggcgggcag aaaaaataac aataaacacc actatcgaca atgctgctac cagcaggata
    26101 acaattaagg aagcgttttc cctgaacatg accatctgtg cccttccata ccatccggaa
    26161 agcaggttgg caaaaatggc aaccaccaat ataactgcca atggcagccc cacataaatc
    26221 tggcgtaata cttttttttt aaccagccgg tttttttccc aataactgat aaaatcggtt
    26281 tcgtgtttgg tcagcataat tcatgcaaag ttaaactaca accgttcttc acacttgcat
    26341 tggtgatata aaatatttat attgtagtct ttaaaagcca aaaccttgaa aataaccctg
    26401 cttcttgctc aataccttta tacccataaa aggctggacc tgcccggtat tggtactttc
    26461 caccttgatc caacagtggc tattgaaccc gaaccggata aaaacaccaa aaaagacttt
    26521 ggagagtcta tcacatttga acacaatccg gcaatcaaag aaaatcaagc cttaattgat
    26581 ttcatcgctt cgcataccgg taaaataaag gcattggccg cagctgacct tgactcgcac
    26641 ctgtcgctgg gtctccagtt tttgaatatc ggcaaaccgt ttatgctgga aggaattggc
    26701 agccttgtta aaacgcaggc cggtcaatat gcttttactt caggtcagtt aatgccggaa
    26761 gtactgaaag aacaaccttt aaaacaaact catccggcag agatacagga agaagcattt
    26821 tccagttata aagacattct caggccccgg aaagagaaga tgcaatggaa aaaaccggta
    26881 gcgttctttt tagtagtggc aggaatagca cttgcgattt ggggaggata tacaatctat
    26941 aaaaattcat cggaagaaaa tgaaactgtt tccacagtag aaaaaaagaa aattgaagag
    27001 gttatacctg cgactatgat aatggacagt acagcaacaa aagacactct actgcccaaa
    27061 aaagaggatg ttgccgggcc acagccaatg acagtacctg ttggtaatta taaatttatt
    27121 gttgaaactg ccggcagaga aagagctttt aaacgtttca aattacttaa aagtttaccc
    27181 accaatattc aactagggac cagtgattcc atcacttttc aattattctt tgtgttgcct
    27241 gcggcagtgg ctgatacagc caggatcatg gattcacttc gtattcatta taccccaaaa
    27301 tggagcaggg cctatattgc acaataaaaa aaggttgcca aaattgacaa ccttttgagg
    27361 tcccgagcag atagtaatag aactgtcttc aacatgagct gtatctgact tatcgcatga
    27421 ctgactgatt gtcaaggggt tccatttagc taattttgtg ttactcctgg ttaatttcaa
    27481 caaacgtaaa catccacttt accgggtttt gccagtcctt gcctttaaaa ctgccaatag
    27541 gtactttaag cagaactgaa tttattcctt ttttaaagtg tatgctggta ggagtacggt
    27601 attcataacc ttcatcaatc aatgggattt cggagtggcc tttttgtccg gctcttttcc
    27661 aatttggcgg cgaaacaagt tttccgttta cccaaacaac actgccttta ctatcccaac
    27721 catccctggg aggtgaatcc gtggcaggtg aacgggaaat attattaaaa ccaatccaga
    27781 agtttttggt tgcctcttca tcactccata tggttgtagt agcataccat gttgtctttt
    27841 cctcgggctt ttccaatgct gcttttatta atggagccca ccaatgtctt aatactactg
    27901 ttcctccaat tatttgtttg gctgattttt cactatcagg atgcaataag gtattttcaa
    27961 tttcaaatgt tttgaataaa tcttcaccat tataataagg tccatacaaa tgccataaca
    28021 aatttgactg ttttacgtag ggaaaaggtt tcaatttaaa atacaatgct ttttgttcaa
    28081 gtagcctact ctcaaagttg ataaatcctt ctttgtcacc gtcatcaata tttgaaatcc
    28141 agccactttg accgcctcct ttccaggctc tttcagcaaa agttaatatc ccgggataaa
    28201 ccggattcat ggtgagcaca tcctcctctt tacccacttt cctgtcgtgc cacatacaaa
    28261 gcgttgcacc aatagctaag gagtcacctt tttccttttc acaaattttt ctgttgaaaa
    28321 tagttacaac agattccagg gggtccaaat gattcaggta aagatgtctt gaatcaatat
    28381 attggatatt cttattactg ctgagatgcg aattatcatc catccacaac tgtcttatgg
    28441 tattatcagt aaagttgccg cccggttgcc aacctatcac ttttttaccc aaactttcaa
    28501 tgtacttggt tacttcgggg atgaaattct tattgataat ttttacttca tctgcgccaa
    28561 tatggatata ctgtacatca taagtagtac aaaactcttt caaaatattt tttacataca
    28621 ataatccaga atcactttgc atatcagttt tcatggctct tttaaaagct gcactatgac
    28681 cgggcatatc aatttccggc acaaatgtga tatatctttc tttgcagtat gcaatcaatt
    28741 cttttatctc cgcttctgaa tagtattgtc ctttatttcg cagcatcgtt tctggagttg
    28801 ttaattcagg atattgtttt atagctattc gccaggcaat atcttctgtg gcatgaaaat
    28861 gaaaaacatt cagtttataa gccgccataa tatcaatttg ctgctttaat agatccattg
    28921 acataaaatt gcggcctaca tcaatcatat agccccgcca cgaaaaggct ggtgtgtcgg
    28981 tgatttcaca ggcttcaatc attacgccat ccctcattaa ctgacgcaaa gtttgaatgc
    29041 cattgaaaat gccatgcaat gtaaaggctg acaaaagaat tctatttttt gaaacactta
    29101 aagtgtatga ttccattgaa attccgggct gctcactctt ttccaaccgt agctcaatat
    29161 agacttcatc ttttctggct ttctctttta cctccggttg acgtcctgtt cgggccaaga
    29221 gcttttttaa gtcttcaact tcaccggtaa gttttttatc atttacgaca attaccttgc
    29281 agctatacag cgggaaatag ccgtaacccc aggttatctt ttgcggtagc gggagcagtg
    29341 aaggcttttc ctgtaattgg tagacataag gaaaaatcag gtgcttccaa agtatgtaac
    29401 cctctcctaa cagatgcagc ccatcatttg tatatttcgg agctaacttt gaaagtctat
    29461 tacagaaatt tgagtagagg tctatatagg tataacgata agatgctgcc tctttactga
    29521 gccggatatt aacctttaga atagaatctg ttttactggc atgaccgatg aatttcccaa
    29581 attgatttga tacaggtaaa atactttgga tatataactt agtggatggg ctcagctgct
    29641 tgaagtattc tgccgttagc aaaatatttt ttaataaact gtctgtactt acccctcttg
    29701 ccaaatcatt tataccaatc attaaaaaca cctttccggg tttctgtttt gctataccgg
    29761 caatgcgata caagacgcct gcgctgatat caccgctgat accccggttc tttattttgc
    29821 tatcgccgaa aagttcgtgc cattcggccc catcagtaat actattccct aaaaatacta
    29881 tatctccggg atttaccggt gtgagttcaa acagacttag ccgctgatga taataagtgc
    29941 tgaataatga gtcatgcagt acaggtagtt taacctgggc cagtaccggt gatgataata
    30001 atacccaata gagaataaac aactgcttaa tagctttcat atttagttca gatgagagag
    30061 aaacaaaata aattaataga atgatttctg tgttactata taaccggtag gacgtacaat
    30121 ggtaacaatg catttccctt caaacaaatc atccacttca aaattgatac gctgcccggg
    30181 ttttattact ggcaaatcgt acactttggc agtcgcatag ttatcggctt tttctgaaat
    30241 ataaagctta taaccgctta ctgtatgctg cggcaaaccg atacttccga aaatcaataa
    30301 attcaatttt ccctttttcc attgccttgc ctgctgcacc tccactggtg aagatagttc
    30361 ccgcaatatc ttcatggaag gcttgggatg accatacata tcatagatac catgcaccct
    30421 tcttctgaac ttattttttt ctgatgtacc aagataatgc gttcgataat ctgtcaaatc
    30481 aaaatagata gcaccttcca catatggttt gctttcatag atagccatat gataaatcaa
    30541 atcttccaac cgtcgttcat caccgccctt gaagttaggc tcacataaac caaactccga
    30601 aataaagaaa ggtttatcgg gatagctaat gtgaacactg tcgagataaa gatgaatttt
    30661 cccgacaggt acatcccacc aactgccccc gtattcattc atcataatgt aatcgccatc
    30721 actggctgca tcgggtgtaa agcgggggtt attaaagaaa gtactggtta gcgtattgct
    30781 cacataggct acgtgacggg aaggatcaag ggtatgggcc ttcgcaatca aatctgctat
    30841 catctttttc atatcagaat cacggccccg caattcatta cctacacccc aggcaaaaat
    30901 gcttggatga ttgtagtagt tataaaccat cctttccagt tgcttcaggg caatactacg
    30961 gatcgtatca ttggaaggtg tttcaggtcc ccataacggc acttcctgct gtatgatgat
    31021 tccgttgcga tcgcaaaaat cataaaatac atcagcttgc tgaaaatgca ggcgactgaa
    31081 aatactattt acgtttttta ataatttacc cattgcaata ataactgaat cagtttcagc
    31141 aaatccgaag tctggattgg atccagccgt ccattctgta cccatcagct ttatacgttc
    31201 gccatttaaa aatgtttgcc cactctcaaa tttcacttcc cggaagccaa tatgggtgga
    31261 aatcttatca gttaccttcc ggccgctgag aacagttaca tctacacgat acaagtttgg
    31321 taaatcaaaa tgccagggat gtactttagc gaaattatat tcgatgatag cttctctatt
    31381 ttgccagaca ggtttgatag tagattgcag aactatattc tttgtaggtt gattttcttc
    31441 tgttatggta attcccagct caacattttt acttactatt gtttcaaagc ctaattttat
    31501 tttcagttta ccggaactat ctgccacatt tagtacaggc gttgcatgga tataagctgc
    31561 tgctggcagg ccactcacaa tcagcgctac cggacgaatt ataccaccat cgttcggcca
    31621 gtcgaaagaa ctgccaaagg gcactttatt ttttccatag tcattattga cagcaatagt
    31681 gatagtattg tccttaccat atttcacccg gccattgagg ctgacaaaaa atttatcaaa
    31741 gccagcacct atgttctctg caatcttttg gccgttaata tatatataac aggtatggtt
    31801 gatagcacct aatagcagga caacattttt atttttccag ttggctggga ccttgatacg
    31861 cttttgatac cagccccatc cataatggtt ttgattatcc ttttcaatat tccatgtatg
    31921 cggaagtcgt actattcttg catcattcaa tggtatttca ttccatttcc cctcaaaacc
    31981 ttcggctttt ttatcaatgg aaaactgcca gtcgctgtta agagtaattg tatcccgctg
    32041 agcaaaagtg gtagtattga aagacaaaac aattataatg gctatataac tatacttaat
    32101 attcatgccc ctttacattt aatatttaac cccttttcat gcgttataaa aaaatcatta
    32161 ttcaaaattc atccagtagc tataccggta atctttaaat ggcaccctgt attgtacaag
    32221 cggcatggaa ccccagctat caattccctg caaaccggtt tgttgtaaat caatgtgtaa
    32281 atatgccagg tcccgttttg ttaattcacc actgtgatat tgttttttgt ttatttccgg
    32341 atctaaatca tccaacctgt atggcaatgc ggagaaactc aaaaggctgt ctgcaaaagc
    32401 aaaatgaatt tttctccccc ttgcattgct gaacgtaacc cagcgaacct ccgtatggtt
    32461 accgctttcc tgtggacggg cataaggata atattgctca tccagtgttt gcttgtattg
    32521 accaaggaaa gtatgggtct tcctgtccca gtaattttct ccggggccgc ggccatacca
    32581 ttgaatattt ttgaaactcc catggatagt aacatcgttt cctgcccgca gcagcaaaag
    32641 atgcttccct ctaagggcat tgaaataatt gtcaactttt acagtgccat tgctgtaaat
    32701 agtgaatacc tgcctatgtt ttgcatcacc attcactact tcctttgtga aactgatttg
    32761 acactgctct tcacttattt tggttatagt agttgtaatt gctgtgccat tttcatacgc
    32821 attgcgccac tcccgcaggc ttctgttaaa acctgcgcca atatcattgt cagttggtgc
    32881 ccgccagaaa gaaggtattg gcccctgctg catcacttcc tctccattca acttataatt
    32941 tactaatatt ccatttttca aatcaaacag gatggaaaaa tttttcccgt taatgctgca
    33001 ttgcacttca ttttgctgaa tctttatggt tgatcgggtc gaaggattaa ctgtgccggc
    33061 aggtaacatt tttaacaaga actgttcagc agcaatttca aaatctttgg gtaaaaatgg
    33121 ctcatccgtt ttcaaaacat aataaatgtt caattggtat tccttgtcgg actgctgtac
    33181 agatttaatc ggaattaaaa tatcattttc ctgttgtggc agtaaggcaa tggatccaat
    33241 tgtacctgtt tctttagaaa gtccattttc ctgcagcacc caacgcagca tcacattatc
    33301 cagcgattta aagaaatatc cattcttaat atttacgcta ttattaccct tccattttgt
    33361 tttaatatgc tgataaactt ttttaatttc tcctgccatc ggggtgaggt ttcggtatgc
    33421 atccactacc ccttttacac aaaaattatt atcgctcaag ttttcatcta ccgggccctc
    33481 caatgggaag tcgccaccgt aggctttgat tcgctttcca ttctttacgg tatcaatact
    33541 ctggtcaatc cattcccaga taaatccgcc ttgtagtttc gggttctgtt caatcacatc
    33601 ccagtagtcc tggaaatttc cgaggctatt gcccataata tgtgcatact cactttgtat
    33661 atgcggaatt tttgcatcgc ttgtagagta acgcaacatt cctcccggag aaacatactg
    33721 aggacaaaat atttcggaga aatgagtatt attcggtttg ccgtaatcaa tatcccgttc
    33781 atattgaatg ggcaaattag tttgttgcct tagccaatca taggctgcgt aaaaattact
    33841 gccgtcaccg gcctcattgc ctaaagacca tgtaacaacc gaggcatgat ttttatcccg
    33901 ttcatacatt cgttgtatcc gttgcaggtg tgcctcccgc cacaggaatt tattaccaag
    33961 agttatactc aaatcatagc ccaaaccgtg actttcaata ttagcttcgt ccaccacata
    34021 caagccatac tcatcgcaaa gctccatcca atatgggtca ggcggataat gtgagttccg
    34081 cactgcatta atattcagcc tcttcatcat ttccatatct ttccgcatat catctttact
    34141 taacgtatgt ccttttcttg gatgatgttc atgccgattt acccctttaa gaaaaacccg
    34201 ttgaccattc actaagaaat ctgttccttt tatttctact tttttaaagc caatgcgctg
    34261 cggcaccact tcaagcatat tgccatcttt atcttttaga ataatgaata aagtatagag
    34321 attgggtatt tccgcattcc aactcataac attagtaatg acagtactga actgtacctg
    34381 tgttttataa tttcccagta cagttttaaa gtcatgggtc ttatcctggt aaacaatctt
    34441 tcctgccgga tccagaaatt gcagttctaa ggtaaaactg tccatcttgc tcagatagcc
    34501 tttttccaat ttgtaattat ctatttcagt gttgactgac aagaaaccgt tggtataact
    34561 gttatccagt gtagcttcta tattggcatc acggatattc aatttgttgg tacagtacaa
    34621 aaagacatcc ctttctatac cggaaaaacg gaacatatcc tggcactcaa ggtaactggc
    34681 atcactccat cggtacacct ggaaagcgat gaggttttct ccttctttta caaaattggt
    34741 aatatcgaat tctgcagcca gtttactgtc ttcactgtaa ccaacttttt tcccattgac
    34801 ccaaacaaac aaacaacttt tggcattgcc cacatgcaga aaaatctgtt tacctttaaa
    34861 cgcagacggc aataaaatta tcttgcgata agaacctaca gggttgttgt tggcaggtat
    34921 gtcaaatggc ggattcattt ttccataccg caggttatgc ccggcaaaat catatggctg
    34981 atttacatag atcggcaatc cataaccgtt caattcccaa ctggccggga tgcgaaaatc
    35041 atcccattgg ctgtcatcat aatttaatgc ataaaattgt tgtggccgct gatcaggatc
    35101 ttttacccaa ttgaatttcc aatttccatt aagtgaaaaa taattacccg atttttcttt
    35161 atcaaatcct tttgctaatt ctatattttc aaaaccaaag gcgctggccc gcataggcat
    35221 acggttgatg gaaactaatt ccggtgtttg cagttcggca ggtaactggc caatcacaca
    35281 attacttgcc agtaaaataa taaccaatat aaatggcgtc ctgagcattt tgtggtgtcg
    35341 catgaaaaaa aacatcgtaa ataattttat tcttttaaaa atcttctgca atggcttagt
    35401 ccactattat ctcatctgca aatatccacc ccggctttcc ttctccattg tggccgggcg
    35461 ggcaaatatt gttcttcgca gtaacccgaa taaattttac agtctttgcc gggaaagatg
    35521 ttttaaaatc aaactttgca tttttctcat taatactgat aagattgttc acagtttgta
    35581 cttcttcaaa atcagttccg tttactgatg tttcaaattt tactgattgc ggcagaaata
    35641 tccagtcgtt atagttttgc aaacaaccca atgcaaggct ttgaatgttt tttgcttctt
    35701 ctaaatcaat tgttgcaatt aaatctttgc cgctgaagcc gtgccagaat ttcccaactg
    35761 taccggttcc tcttactcca tcagtgaggg aattagagcc atcagccatg tagtaacggc
    35821 ttacggggtt agcgtatatt acatttttac caatgccttt atgcattgca aagctttgcc
    35881 gggctgcctg aacacccatg atgcggccat tctgcattgt ggatgctttt agcaaacctg
    35941 tagaattgat ttcaacaggg gcactgtatt tattgctggt taatgtgggt tccgtgccat
    36001 cggtggtata ataaacttcg ccattgagta tttctgttga aagatttaca aacagctttc
    36061 cgttttgtga aaacggcctt atgtttactg tgaaattacc agggcaataa tgcaaacctt
    36121 tttccccata agctttgaaa tgattttgaa ggcgttcatt aaagctgttc cagtttcggt
    36181 tttcctttgg cgaccaaacc acttccgcaa gggcgggcat gcgtggcagt atcatgtact
    36241 caacatgttc ggctgtggtg ataaactctg tccacaaatt agcctgtgcg cctaaaacat
    36301 gttttgcttc ttctgcattc aactctttag gaactggttc gtaatcgtaa acatttttca
    36361 atgtattcat tcctccaatg gctataggtt ctccttccgg acctgcctga taatggtcaa
    36421 agtaaaccgg cttgccgggg gtcataatta catcatgtga ctgtttagca gcttcaatac
    36481 caccgttctc tccgcgccag ctcatcaccg tagcttccgg tgccaggcca ccttccagaa
    36541 tttcatccca gccaattatt ttcttatgct tactgttaac aaatttttct atacgcttta
    36601 taaaataact ttgtaattct tcttcatttt ttaatccttc cttttttatt ctagcctggc
    36661 acctgttaca ttttttccag gggtttttat ccacttcatc gccaccaatg tgaatgtatt
    36721 gcgatggaaa caaatcaata acctcagtta atacattttc taaaaaatta aatgccgaat
    36781 cattgcctgc acaatagttg gaagaaatgc ctgtataatt gcctcctgtt aacggaaact
    36841 gctgttgttg tttgcagcac aactgcggat acgatgcgat ggcgctggcc acatgccccg
    36901 gcaattctat ttcgggaatg atggttacat ttcgttgcgc agcataagca ataatttctt
    36961 tgatttgctc ctgtgtgtaa tagccgccat aggttggttt ctcatccggt tttgcctgcg
    37021 gacggctgcc ccatatctta tcggtttggt caacccgcca tgcacctaca gaagtaagct
    37081 ttggatattt cttaatttct atccgccatc cctggtcatc taccaaatgc cagtgaaata
    37141 cattcatttt gtacgatgca atcaaatcga tgtactcttt aataagttcg gggccaaaga
    37201 aatgccggct tacatcgaga tgcatgccac ggtatttaaa ccgggggtaa tcggtaatac
    37261 tcatgcaggg tatttcaagt ttcgcattag tacggatggc aggcaatgtt tgcaataaag
    37321 attgtatgcc ataaacaatt cctgcagtgg tattagcttt aatggtaaca gcttttgaag
    37381 tgatgtccat attatagcct tcatctccaa tagttgccaa cttttctata ctcaactgaa
    37441 ttgttgcagg cagggatgtg ttgacaggca aaacaaaccc cgtagtattt tttaattgaa
    37501 gattgaaaaa tttggctact ttttttaaac ccttttgctg tgggttgtac tgaatggcgg
    37561 tattaccagt taaaataaaa tttcccgatt gttgctgtat tgataccggc tgcggaataa
    37621 tatttacggt ttgttgagca acacaaagcg tagtagttaa gcttaatata aaaatgaatt
    37681 ttatcttcat tgtactttta ttttaaatgt tgatgcagga agattttctg aattcacaag
    37741 gttgccattg ctgaatggcg accaaccgta gtaaataact tgtggtttac tgcttacagg
    37801 aatgattatg cccctatcac caaaaattgc ttttgtttca ttttttccat caattgaaaa
    37861 tcctttaagc ggtttatcat ctgatgtttt taaaccattt gccatgtatt gaaaagaaac
    37921 aatcactttt ccattgcgat attttgcgga tagtggaaga gggccggatg gaataatttt
    37981 tttatagtac gtctttttca atgcccatct tgccagtcgt tcgccaacag tttttttatc
    38041 cggtggatgc acatcattct ttaaaccgat gtcgctggta acggccatac cggtattgtg
    38101 ggtttccttt aatagctgta attgattatt cctgaacatg gcccaatact ttctttctat
    38161 tgaagaaagc tgtatccaat aaattggaag ttgcttgttg ctccaaactt ctctgtattc
    38221 tttgatgaat aatttaaata gctctttgta ttcatcaacc cgttctggtt cttctgcatt
    38281 gctttctccc tgataaatca ggattccttt caccgaaaaa tttcttaggc tatcaatacc
    38341 cgctttaaaa gcaaagccgg gtttataggc atgatttggc ccctgttcat cagcaggaac
    38401 attgttattt tttcccacat tctgtgttcc tctttctcta atccatattg gcaatgcatc
    38461 atttgagagc cagtttccat ttactttttt tctgaatgtt acctgctgag ataatgcttc
    38521 tgtactgata aacgtttcaa gtggtgcacc accaatggaa aggtttataa taccgattgg
    38581 tacaccagtt tcagaaaaaa ctttttttgc aaaataatat gcaactgcac tcattaacct
    38641 aactgaaatg ctatcgcact gttgccatcc attccaatca taaaaccttt cggttgtaag
    38701 cctgcttata agggagtcgg tgtaaggcac accaaaaaca ttccggcctg caggcggtgg
    38761 atttagcaat ctaatattgg attgctcagc actatgtatt tcatctttcc aatgttgctc
    38821 tttaaccatt ggccattcca tattagactg acctgtacat atccatacat caccaatcag
    38881 aatattttta aactcaatct gctctgcttc actggttaca cttaaaattt gcgaatgagt
    38941 agttgctttt tgttttggga aagtaacaat ccaaaccgaa tcatttgtga tttctgtagt
    39001 ttttttctct tttgaaaaca agacatttac acttctgcct ggaatacctt tgccccaaac
    39061 ttttattggc ttatttcttt gcagtaacat attgtcagaa aatacttccg ctaccttcaa
    39121 ttgagcggtt gaaaaaagtg gacagagcaa tatggggaat aaaaaagatt tcatctgctg
    39181 tctattgcaa tgtggtacaa atctggtgcg agttccatta gtctttttag caggatagtt
    39241 tggttggatg cccttacaag gttttgaccg ggataggcag ctccataata ttcgtcaccg
    39301 tttaaataat cagtaaggaa ccgcaatgcc tgcatgtaaa tgctaaactt acctgcatag
    39361 aaaaagtgtt tcttttcaat ttctgtaagt tcatccttca tttcactcat ataaccttca
    39421 acaattgctg tataatattc tttacgaata attattttac taaaatcgct ttcctcttca
    39481 cctacaggag ataaatatgt acgcatcata tcacccacat cactgataaa gtaaccgggc
    39541 atcactgtat caaggtcaat tacacaaagc cccttacggt tatgattaaa taaaacgttg
    39601 ctgatttttg tatcgtgatg tgttacccgt tttttaaact ccgggttggt tagtattttc
    39661 tcatattcat ttgctataaa tgaaaattct tttagtgttt caatcaattc aaaagtttcc
    39721 tgattcctca gtttagtagt gtttgcagcg gcggacaaaa attgcaggta acgtaaactg
    39781 aggttatgaa aatcgggtat ggtaatttct aatttatcaa tttcgattcc tgaaagtaat
    39841 ctggtaaatc ttccaaattg tatagctgct tcaaaagcca tttcaagtgt agttgccaca
    39901 tcaaaagtgt gggagccttc aataaaaggg agcagacgga aatacccatt accttctacg
    39961 taaatcatct cattaccatc attagaaatt attggcgcag caaagagata atccgggtag
    40021 ttatttttta gatggtttgc aataagacta atgttcccgg aaatggcagc aggttcctgg
    40081 aatacattac tgtttattct ttgtaatata tattgtttcc caggtgcaga aactttccag
    40141 gtgctgttaa taagacctga gccaaattcc tcaacggtta attcatcttt ctgcaatcca
    40201 taagttgata aaacaaattc agtcatacta tttctgccaa attttaaaac tcatcattta
    40261 atgcaaacac tggtttacgg tacatccatt taccgcctgt aaagtctgga aattctatcg
    40321 tttcgtttcc tttctcaatg gaactttcac tcaatggagt gatggcactc catgcagcag
    40381 catcatacac atccattggg gttgctatat ttcgttttat tgattctaca aaagaatgga
    40441 tcacaaaaaa atccattcca ccatgaccgg caccttctgt ttccttactc catcttaccc
    40501 aaagcggatg gtcgtattta tcaaaccatt cttttgcagc atcccactgg tgtggttttg
    40561 attggccttc tatgtgcaca cctttattta catccatcca aataccgttg gtgccttgta
    40621 cacggaagcc taatgagtat ggacgaggaa gattggtatc atgttgtaat aaaatagtct
    40681 caccatttgc acaaccaatt gatgtagtaa ccacatcacc caatttgaaa ttaatttttg
    40741 cattcggatg ttcaggtcca cattttttta caatatgctc atgcaagcct cttgctttac
    40801 ttgaaaacga agagatattt acaaacctgt ttccacggtt gatgttaatg taatgagcta
    40861 tggggccaat accatgtgtg ggatataagt cgccattacg ataaacggaa tgatgggtgc
    40921 gccaccttgc ctcactccag cctttatcgc caaactcaca acctttgcca taggcatcaa
    40981 tgccattatt gaattttact tcacgcaggt catgctgata accgccttgc aaatgcacca
    41041 tctctccaaa caaaccctgc cgtaccatat ttaaagctgc catcacatcc ctgcggtagc
    41101 aaacgttttc cagcatcatt acctgtgcat tgtattgctc tgttgctttt acaacatccc
    41161 aatggtcttg cagcgttatg ccaagcatta cttcagtacc aatatatttt attcctgcct
    41221 gcagtgaacc gataatcatt tctttatgcc attcccacgg agtggctata attacaccgt
    41281 caatatcttt caactccagc atttttttcc aggcataagg gtcgcctgta aaaatttgtg
    41341 gcattttctt tccgcttttt gtaatcattt cttttgaccg ggaaagcata cggtcgtcaa
    41401 tatcgcaaat agctataagg tctacatctt ttcgccgcaa cacaaggtca aggtggtttt
    41461 gcccacgcag gccaacacct attattacta attttatttt gtctttttga ttagctgcaa
    41521 acaaactggt ggccggtaat atggctaatg aggcagccgc taatgttgtg tttttgatga
    41581 attgttttct gtgcataaaa ttcttttaaa tgtatttgga gatattctca gcataatttc
    41641 cttactagca tagtttgtaa ttgtaaatcc ttcctaaatc accgcctaaa taaagctggt
    41701 ctctttctct caatctgtta acaaagtaat atcctgtgca ggcgcaggat atcctaatgc
    41761 agtaatttca gtgaacacag catctgttgc agactgtgta gaaagtttca ttccccattt
    41821 ttggaaaaag gaagtaagat ttttaccgct gatgttgcaa gccttcaaca taaaccaacg
    41881 cattcttgtg tcggtggttg ttggtctgtt ggtttctctt ctggcctggc gatgcagttc
    41941 atgataaaaa ctttctccaa aggccagttt taattgctgg aacatacaca accttatcca
    42001 tacgctggca ttactgccat tgaaatttct ttccatctca ggacgggcaa gataggctgc
    42061 ggtattattc cattcgtttt gagtagtaag ccttgttggt agtatcccaa acgccttttc
    42121 cactgctatg ctgtaaatat tcactgttac ttcaccaagt tcgctccatg tccatgaacc
    42181 ctgctggtgc atatgcccca actcatgcca gggtccccag gttaagttat cggcttttaa
    42241 aattgccggt accccgccgg taacagaact ataaaaagtg cggtaaaatg tcgctgccat
    42301 aaagtaaccc ggatggtctg tttctgtcat caggtatttg tgcacattgg gctggtcaat
    42361 cgggtcatta ccaaaaaggc cactgatgct gtcttcaata gcaattaccc ggtcagcttt
    42421 ttttatcaat gcttcctggt tttcactttt ataggttctt gcattgacca atgaaaaggt
    42481 gataatagtt ttattgccca ctaattgtac atcgggaaca ttattgatat tatcaaccat
    42541 cttaacccaa tcagcctgtg tggttctgcc caattgataa taaggtacag gcctcatgcc
    42601 agaagtaaat tttaatctta ctctgccggt ggggttgtcg ttatgaaatc ttacatagat
    42661 gataccaccg attgcatctg taatagaatt aacccctgcc gttaatgtat gagaagtggg
    42721 gttccaactg gcctggtaac gggaatatgt acctacaagc aatgtgggga gacgggtacc
    42781 gttttccgct gttacttcaa cagtcattac cgttgatgct ggtaaataaa atccagtagt
    42841 gtaaaaatcg gacgcagggt tattttgtgc cagcctagtt gcttcttttg agccagagac
    42901 tgtttcattc accagtaaaa tactgtcagc tgtatttagt accacaacag gtggcggagg
    42961 agggggtggt ggtggtggag gcgtagtacc cccatcatta ttttttttac atgccgacaa
    43021 tacaactatt gacagtacaa aaactgcaaa cgcttttttc atattggtgt ttttatcttt
    43081 ttttcaaata attttcaagt aacagcatac agtaccattc ctgccgtggc agatgaaatg
    43141 gccctttata caaatttcct tttgccgatt gtgctaatgt gccatcccgg tgtaaatagc
    43201 caaaccattc accgttttcc ttatcatgga aatgactgta tgaataatca tgcaccattt
    43261 tatgccaccc agcatatttc tcattgccag tcattaagta agccagcagt gttgcaataa
    43321 tcacttcgtt atgcggccac cagaatttca tatcctgcca gtattcctgt accggcttat
    43381 tatataaatc acggaagtag agaattccgc cgtaatcttt atcccagcct ctttcccaca
    43441 tatagtcaat catacggcat cccaaatcaa tcagtcgctt atcattattc cggtattttg
    43501 cttcatgcag aataaaccat gcaccttcaa tggcatggcc gggatttaaa gttcgtccgt
    43561 ctatatggtc aatgatgctg ccgtccgggg ctacctgttc catcacacat tgtatataat
    43621 cttttacaaa atctctttct atttcagcca tccatttctc tatccattca tcacagcggg
    43681 ggtcgccaat ggtttctctt aattgctgtg ctgtattcat cattatcata ggcacaccta
    43741 tacctttgga tggccgggtg tttgtgaatt tgggttgcag cagccccggt atagttgcat
    43801 actcaataca cttaccaaaa acaaatcttg cttttcctgc ggcttcttca tcaccacttg
    43861 cctttgcata agccgcagca gctattacat aaaatgtttc agaaaaaaaa tacctacgct
    43921 ttcttattgg tcttccatct ctggttacat gaaaaaacat ttgcccatct ttatcaaaac
    43981 aatgcttgtt taaaaaatcg taaccaagct tagctccatc aagccattcc tgtttttttt
    44041 caacggtgtt atacaaagtt gaaagcatcc aggtagccct gccttgtatc catacagctt
    44101 tatcatcatc aattaatgaa ccatcggcat ctctcattaa cagaaaccct ccacattctt
    44161 tatcaaatga acggggaaac caaaatggaa tagtattggt caataattga tttgagtaga
    44221 aaccttttaa ggcaattaaa tcttcctgag aatacatttc agcttatttt ataatttcat
    44281 ttaccgatac ttttacaaaa tataaatcct tagttccttc atataatatc ccaatcgtat
    44341 tatcatcaat ttttgttaat gaggagtaac cgtaacactg tctttcatca attaataatt
    44401 gattggcagg caaccatgtt tgtcctaaat ctaaacttgc tttaattgta atattgtagc
    44461 ggccagattt agtgctggga ttgctgaaaa acaaaacatc tttcataatt cccttcacca
    44521 tcacttttgc ttttatgata ctggccatac aaacagggtc gggcaatgca ttgtaagaag
    44581 tagaatgctc cgtccatgta gttcctaagt ttgtagttgt ggctacactt ctaaaacttc
    44641 ccctgttatc tctcatattc agcatcagtg tacccggttc agtttccacc acctggcttt
    44701 cagtagtgtt tgattttggt ccatttactt ttcctttcca gtttgtaccg tggtcatcgc
    44761 tataaataat ggttgaataa ggcatcttgg tttcattcca gtattgtgca gcaaaaacta
    44821 atttgccatc ctgcatagca ataccgctgc ctgggccatt aaaaaataaa tgccatgccg
    44881 ggtttttaac ctgtggtgta attgtgtatg gggcagacca tgttaggcca tcatcgttgc
    44941 tgcttactaa cacaaactgg cctgtagtgt ctggtgatat accgggtaac gaaccggcaa
    45001 tggaacgatt gcctttactc cacagtgcag caacccatat ttttttagtt accgggtcaa
    45061 acaaaataga agggtcgcca acgccattat tttcatgtgg cgcacccata tccattatta
    45121 ctttcatggg ctgccaggat ctgccgctgt ctgtactgcg acttaaacct acatcaatat
    45181 tgccgggcaa gtctgcactg ttgttgtagc gaatgtcata tacagaaagt aaagtgcctt
    45241 tatcagtagt agcaatgccg ggaatacgat aagtgttaaa gttgtcatcg cctgccttac
    45301 gaatggcaac gccggtacgt ttagcataaa cagaattgtc ttgcttaacc agtaattcct
    45361 tatttgcatt atcaatcagc tttgtacatc gcaattcaat tttattatca atagtagcat
    45421 cactctttaa tacaatactg aaccaaataa aatggatgcc gggtttaagg ttcagattca
    45481 cagccagatc aaacgaggct gccgatgggt ttactgaagt aacaggattt gcaatagaaa
    45541 atggctcacc acctgtgagg taaacatcta tcttttgaat attgctaatt gcgtcagcat
    45601 tcaatgtaca aattattttt ttgtactgct gttcgctatt tcccgagggg attgagaccg
    45661 ccacccgcag caccggatta gcggttaacc ctttcagcac aggtgtagca ggtgcataag
    45721 cagaagcctg aattgccgca ggtggtggag cagttgattt ttttgaacac tcaaaactac
    45781 tcagtaataa aaagaggaaa catagccctg cattaaaaat tagtttttgc ataatcttat
    45841 tttattcttc agtaaagtca ttagcttttg gtctcagaaa agaaagctga ataattaatg
    45901 ctactaacac aataccagca agcatcgcaa aatcttttcc taaatttcct ttatcagttg
    45961 acttgccaag taaatcagtg ataaaagctc cggcaaatac acctaccata ttcatcaacc
    46021 catacgcagt agcacggtat ttctttgaaa caaactggca gagtatcggc atattgttgg
    46081 catcaaacat tccgaaacca aaaccaaaac aaaatgctgc acctattact gcaaataatg
    46141 aatggccaaa tccaataaac aataatgcag gaatggttaa tgccaaacca atggcactgg
    46201 tatatattct tccacgaaga tttttttgta cccacctgtc ggataaaata cctccgaaaa
    46261 taacgccgat aaaagatgat gccgcaatgg taatagtgga gattggtcct gccttgctca
    46321 tatctatacc taagttttgt gcaaacaaag taggcaacca gtttttaacg ccccaaccgg
    46381 gcaaactggg cacagcaaaa taaaaaagta taatccagaa tgaaatatta gtgaacaaaa
    46441 tgcccaatcc tttaagtaat ggagtcttaa ttttttctag attgttgttc tctgtattta
    46501 tttcatcaac aactctcttc tctctcagta atactattaa tacaacagaa taaacaatgc
    46561 caattatacc aaatgaatga aatgcctgtt gccaggaaaa tttatcagcg atggtagcgc
    46621 caaatcctcc caatgcctgc cccatatata agccggtcat gtgtatgccc accgccagcg
    46681 accttgtttt tgatgaatga taatcggcaa ttaatgataa ccctgcagga atgtacaatg
    46741 cttcacttac acccattaaa gcccttaacc aatacaattg gtcaaatgtt tttgcataac
    46801 ccattgtaaa cgtaacagca ctccacacaa acaaactacc aacaatcaac catttcctgt
    46861 ttatcttatc agctatgatg cctgatatag gactcataaa cccatatatc cagaggaaaa
    46921 tagccataag ataaccgaag ttggctgccg tttgcaattc atgaatatct atttgcatag
    46981 atggcttcat ggtgcttagc atctgcctgt ccatataatt caataatgca accacccaaa
    47041 gcaaggccac cacaatccag ggatattttt ttgattgtat catttattct tttgtaaaat
    47101 ttttaccccc agtatattgg gcgaacgaac gccagccttt atttcccctg acggaataat
    47161 gatccctgat ttccattgta cggcagtagt ggtaacaggt gtatcaaacg gaatggtgcc
    47221 ggcaatattc caggcatcat tctcaatatt atatatcaaa acttcattgc taaacccggg
    47281 atgttctgat tggagcttat ttttttcctg gattagctcc tgctttttta ccgggtcttt
    47341 ttccgcattg attgcggcta tcagtgtctc tgtcttatga aaaacgattc ccttatctcc
    47401 tccaaacaga agtatatttt ctgcatcgca tactattcct gttcccgcac ttaacgcata
    47461 gggcaatgcc tttttttctt cccaggtgtt tgtgattaca tcatatacaa aaacactatt
    47521 gtaaaaatca ctgatgccac tgttattttt ctttctgcca ccgcataaaa atattttatc
    47581 gcctgtttta ccaggtattg ctgttactac tgtatgagat acaggctgag ggatatcagc
    47641 cagtttattc cagcctgctt ggatgttgac caggtctaag gcaagcaatt gagatgttgt
    47701 gtgagctgcg gtttcacccc ctgctataaa tacagtagtg ccattcaata ttgctgaagc
    47761 attgcttact gccaccggaa gaactggcag cgacttgaaa aggatttccc cggccctgtc
    47821 atcccatatc atcagccata ctttatcact tatacctttt tcactttcgc caccggcata
    47881 caaaacacca aaaggcgtag aacaactagc aacgtaggca attgagtaag ggagtgaaaa
    47941 tgattgggat gataacttta gtttatgccg cttcttaaaa taaacattaa cggtagtata
    48001 aaatttcttt accccccctt cccatggcat tcctgcgggg aagttggccc cacccgcaat
    48061 aaataataca tttttgtgat aaccagtaac cggacctgca acacccagtg aaatttgctg
    48121 cccactatcg gcaggcaatt cgccaacaat tttccatttt aataaaaggt tcgggttttt
    48181 ttgtgctact gccggaatag ttaaaaacat aagttttaca ataacagata ttttcaaacg
    48241 gaacatttta attaattgct gttgagtcaa cgggtatagc cgaacagaaa gtactgaacc
    48301 caagctgcgc tgtttccttt ttaaacgatt caaactcggc actattcata tttttaacag
    48361 gtaaccgaaa atcaccacag tccagattga ttaatttcat aaacgctttg ccggttgcta
    48421 tgccaccata tttaccaagt aagcgaatca tatcaataga ttgctgctgc aggaattgtg
    48481 ctttggctaa atcgccggtg ttataagcat caatcagatc gtaatataat ggtgctgcat
    48541 aattataggt gctgcccaca gcggcttttg cgccaagagc taatgccggc aacatatttt
    48601 catccctgcc ccatagcata tcatatttac cattttcaaa atttatacag gaaagaaaat
    48661 ccataaaatc ttcatgggta tattttattc ctgcaaaatt gggtattctt ccgtctattg
    48721 cttttagcaa atcaaacatt gtaaaaccaa cacctgtcag taccgggata tgataataat
    48781 aaaatggcat acccggaacc atggaagcaa cttcagcgca acattgtgcc agcatttcaa
    48841 cattcgccgg tttaaagtaa aatggcgctg taaaggaaac tgcatccaac ccaatttccc
    48901 ttgcatgcaa tgccagctct ttgcaatcag caagacaagt gccgcctaac aatggcataa
    48961 caagaaagtc gctatcttcc ctggtacagg cagcccaagc ttcagcaatt gctttctttt
    49021 cattgattgt cagtgagacc ccttcaccgg tggaaccgca aataaaagca cccttgatgc
    49081 catttgattt caacatcgaa tagtaaagcg gtattaaccc gggattcagt gagccatcac
    49141 tatgcatggg tgtaaatggg gcagctataa gcccctgtaa atgttgtcgg accattttac
    49201 ttttgtttaa gtagagaaat acaaggggta ttttactacc ccttgttcac caaaactact
    49261 gcttatgcct gcttatgaga aaacagaatt taatacccgg atgtttgagt aagcaaagga
    49321 tcagcagtta acataccctg agaaagcggt aataaaaact tagctgtact agccggcgaa
    49381 aaataggttg ggttcaaatt cgcataaaca tacgaatcac ctgcccttct taatgcccac
    49441 cagcgtttcc cctccgcaat aaattctttc agatattctt ccaaaatggc attcatgttt
    49501 tccgtaatac ttccatttac ataaggtgta taacctgcac catatgccct taagcggatg
    49561 gcattgattt ctgcagaagg atcttctccc aacttggctt ttgcttctgc tagtaataac
    49621 aatacatcgg cataccggta aatgggataa tcattattat agatttgaga agttcccgct
    49681 gttgttccaa tgtactttgt tagcaaaata cccctgatag catgaggagg tgcagtagaa
    49741 tacatgaccc ggaaagtatt gctgattcgt tggtcagcag gcagggcagt aagccggtta
    49801 atcatggcct ggttcatacc caccctgtta gcactattta cgtaaggata aacagaagac
    49861 actgtaggtg tgggggcctg tgtaaaagac aaagtaccgg cctgaattga attcactaaa
    49921 aaactaccaa aaataccatt ctgcgcctgc tgcaattcat agttaatagc gaatattatc
    49981 tcactgttgt ttacttttct ggtcgggtca aaaatatgag cataattagc ctgaaggcct
    50041 aatgaagagc cctgcagatc tctgacttcc tgcaaagcat ttttcgcagt tgtcaaatcg
    50101 gtagtaccac ctcccatgtg ggtaccagac catataaaca catctccttt taaaacaaga
    50161 gaagctaccc tgctccagta aacccttttg gcagtggata aggtatttgc agaaccaaat
    50221 aactgaagtg attgttcaat atcactttta atctgtatca taattgaatc agcagcggtg
    50281 cgaggcttat acgtttcagc ggtattatta attgtttcaa ccggctcagt atttaaagga
    50341 actgcacccc aagtcttgag catggtgtaa taaacataag cccttaggcc atatacttca
    50401 gcaagtatcc tggttttttc agcatccggc aatggtgttt gcggaattat tttaataaca
    50461 ttattaaaat tgtaaatcag gttataaaag ccaccccagt tggtaaaagg cacattcaat
    50521 gtactgatat tgtgcctgta tagattctgt aatcctccat ctacagattc ggtaaaaagt
    50581 ccatcaaccc aaatatctga acgcatttcc cccaactgga aaaatgtacc actgtaagac
    50641 ctgaataagc catatacacc atgatacaat gtcctggcgg cattaggatc ttttaatgcc
    50701 tggtctttgc tgatagtatc ctgtggaact acctcatcca attttttggt gcaagagaga
    50761 acactcaaca ttaacaagaa tgctccagtt agtattaaaa atttattgtg tttcatttca
    50821 agaagttttt tgtgttgaat aagcaattat aagatcaccc gtacgccaat tgtaagccgt
    50881 ttcggcaacg ggaatttacc ctggtcaaat ccgcccactt ccggaaaatt gccgctgtag
    50941 cctgagaaat aaacaagatt ggttccggtt acatttacac tcagtccttt taacctgtta
    51001 cccaggtacc tgctgagaat ttgtgatgac aggtcatagg ccagtgtaag ttccctcaac
    51061 atcacataat cgcctttctc ccacaaattg gcagcagggt tattaccact agcatctgtt
    51121 gcatagtttc ttccctggtt agcccaataa taccttggca ttgtgccatt aggattagta
    51181 ggtgaccagg tttttaatac gtctgttgtt gagttttgag aaccctgcac ctggctaaga
    51241 cctcttacaa tctggttatt cagaattaca aaaccaaaag catagtcaaa cccggcatac
    51301 aaacgaagac ctttgaatga actgcttata ttaaaaccac cgatatgctg aggagtggtt
    51361 cgacctacct ttacaaactg gcggctgtca atggtatcat ttttattgac ctggtgccac
    51421 tgagcgtctc ctaattgctt tatccgctta ttcgtataag gcaaaaaggc attgtagaca
    51481 tttgcatcag ccgtaatttt agagagatcg gtataaattc catcccactt tggagcccat
    51541 acttcatcga gtcctatacg ctgaccttcc actaatcctc ttacatacat ctgttgtccg
    51601 gggtttttag gatcccacac aaaaaatcca cttgttctgt tgcccggcaa cccattaaag
    51661 ggaagttttt tagcaaagct ttttacatga aaataattga cccctgcttc aaggctaaat
    51721 ccatcagcat ttttggactc aattattttg gcatttactg ccaattctat acccttgttt
    51781 tgcaactggc caaggttagt agtaaaactt ccaaaccctg tttgtgagga aatatttaat
    51841 cccgcaagtt tatcaaatac attacggata aagtaatctg cagttaacgt aatcctctca
    51901 tttaaaaatc ccaggtcagc accaaagttt aaactgttgg tacgttccca tcttacatcg
    51961 gaattaatat aagatccggc ataagtacca ccaaagccat tataagtacc ggcgttgtta
    52021 tatacctgtg aggttgcaaa aaagccaaga gagcttacat ttccgttaac accatagctt
    52081 attcttggtt ttacgctgct taaatatttt gaaataaaag tcccctggta aaagttttcg
    52141 ttgtgcaggt tccatcctac agaaacaccg ggaaatgttc cgtaatagtt tcctttttta
    52201 agtctgctgg agccatcaac tcttacaaca cctgtaagaa gatacctgtt tttgtaagta
    52261 tagtttaccc ttccaatagc ggatgtaatt ctctcccatt ggttaaaatt agaagaagca
    52321 cctgccgggt tcacaatagt tgttccctgc acactgggtg gagtagaagc cgtgagccag
    52381 ggaattaaat cggttggtgc cccttgtgaa aaaccagagt ttacataatt cttaaaatca
    52441 taaaactccc caccagctaa tgcggtaaca gtgtgtccct taaagctttt agtaaactgc
    52501 aaaaaagcgt tggtggtgta ttgggtggtt tttgtattgt taaagctggc ggcccgatta
    52561 gtgttaaaag cgccaccatt tccctgctga tatgccttgg taaaaaaatt gttattggtg
    52621 tattgataat acccggaacc gctggccagt attttcagga atggcagcac tgtatattca
    52681 agattaatac cacctaagaa acgctgttgg ttggtactgt taacagtcag gttgctccaa
    52741 tacaaagggt tacccagggt tacatcattt ggaccaggta agattgcacc tgaagtatcg
    52801 tttacatatc ttactgttgg agcaacacct acaaaacgct gcaacaaacc tcctgccgct
    52861 cctccttcgg ggtcaacatt accaaattga ccatagggca aagcctgttc tacattgtat
    52921 gcgccaatat tggttgttat tttcaggttt tgaccaacat ttagcccccc gttaaagttt
    52981 aagttaatcc gcttcagcaa agaacctatt atcataccat tatctttcac cgttcccagg
    53041 gacaatgcat aagatccctg ctcattagca cctgaaaaat taatattatg ttctttagta
    53101 tttacctgcc gtagaagcat agcctctctt tcctttgcac ttaaatctgt aaatagtatg
    53161 ctgtctaact gagaagtgtt gaaaggatta ggatctacca atagtttcca cctggcatta
    53221 ttcaacaaat ggcgattctg attatttaat agctgtgtgg tgtacaaacc ttccgcagat
    53281 ctccagcctg tattaacagc ccaaccccag gctcctaaaa gctggttgcg atctgtattc
    53341 atagcattga tattgttatc caacgagtcg cctctgaaac gggcctggat tccgagtcgg
    53401 ttcatccgaa tatagtctgc agcactcagg tattcagaag gattagtacg gacaaagttt
    53461 gtagtgtgac tgaaggaata agtaacctgt gcttttcctt ttttgccttt tttggtagta
    53521 acgagtacaa caccattagc agctcttgcc ccatatatag ccgtagtggc agcatctttt
    53581 aacaggtcga tacttgctac atcattaata tctaatccat aaagtgaggg aacgattaca
    53641 ccatccacaa tgaagagcgg cgtaccgcca ccaccaaatt cagtacctcc tctaaaggaa
    53701 attgcaggag tggttcccgg ctggccggtc cgttgctgga tcctgagtcc tggtgcattt
    53761 ccctgtaata cagtggcgac atttgtagaa ggactgtgtt caaaagtttt ggggttaaca
    53821 gtaacaacgg caccagttac ctcttttctt ttttgagtac catagccggt aacaacaaca
    53881 gcgtccagtt cactgacttt tgtttcaagt gataaatcaa ttgtggtgtt ggcgcccact
    53941 ttcacctccc gggtagtaaa cccggttatt gaaaaaacaa gcacttctcc ttgagagaca
    54001 tcgatggaaa attctccatt gatatttgtt tgcgtaccga cagtttttcc cttaatgatg
    54061 atacttacac caggtaatgg tttgttatca tcactcgacg ttatcttacc agaaattttt
    54121 tgaacttgcg accagactgt cgcagatatg agaaagctaa caatcagtag ccaacccttc
    54181 agacaagcag atttcataac agcagtttat ttgttatttg tattatgtat gacataataa
    54241 aggtaaattc atttattttc cccaaccaaa ttttttttta atattttcgt atataattga
    54301 taatcaagta ataaatatct tttaataaaa tttcttactt ttataaaatt tattaagtaa
    54361 tacatattaa tgatggaaaa ggcaactact gatactttta aatccataga aaatacgagc
    54421 ctagtagata aagttgaagc caatctcgta gagttactac aacaacagaa gttgaaaatc
    54481 ggcgattcaa ttcctacgga actggaattg gcagagaagc tgggagttag cagaactgta
    54541 ataagagaag ccttgcttag attaagaatg atgggattaa ttgaatcaaa aaagaaaaaa
    54601 ggtgctgtta taacgagccc ggacttgttt ggtaatatga gtaaaagtat gaaccctcat
    54661 attcttgctc cggaaacact aaaagaagta tttgaaatac gattggtatt ggaaatagga
    54721 atggccgacc tacttttcaa tcgtattaca aaagaagatc tgaaagcatt acatgagatt
    54781 gttgatgatg agccaccggc tacacaatac catttattca atattgaaca tgaaattgca
    54841 tttcatggta agttgtatga aataacgggt aatgaaacat taaaaaagtt tcaaaaaatg
    54901 ttattgccgg tatttgatta tgtacataac agcggattgt tgaaaaaaca gtccttgtta
    54961 aaaatgttcg tttcgcacaa ggaactggtg agtattttag aaaatgggac acctgaagat
    55021 ttcagaaatg gtatgcgaaa tcatttagaa aatcactttg ccaggttatt tgacttatcg
    55081 taagcttttt tactcccatt actgatgaga gacttgtact taaaacttac acagctcact
    55141 tttatcacta attttcctgg ctgaagactt tactgatttt tagcatactc cgggactgaa
    55201 ttattgcagt tgcatgccag gtaagatata taaagaagaa gggaaagtat ttcgcataaa
    55261 cacgacttac tttcccttga ggtcccgagc ggattcgaac cgctgtagga gcttttgcag
    55321 agctctgcct agccactcgg ccacaggacc ttttttctga ttctgcttgt cagcgaccga
    55381 cgaaaagcga accatttttt taaaccgctg cgaaaataaa gcatattttt gaaataggtt
    55441 tatttacatc atattaaact agttatatga gccggatcgc agaatcagag ctgatcatta
    55501 acgaccgcgg ggctgtgtac catattgacc tgcggcctga tgaactggga gacaccgttg
    55561 ttaccgttgg tgaccccgac agggttagag aagtcagcaa atattttgac aagattgaag
    55621 tgaaacgcca gcaccgggag ttcataagcc ataccggata tatcggcaaa aaaagaatta
    55681 cagtcctttc ctccggtatc gggcctgata atattgatat cgtcattaat gaattggacg
    55741 cccttgtcaa tattgatttt gagacccggg aagttaaaaa gcagctcaaa tccctcaata
    55801 ttatccgcat cggcacttca ggttccttac aggcggatat acccgtagat agttttgtgg
    55861 cctctactca tggtttgggt attgacaacc tgcttaattt ttaccggtac gagcaaaatg
    55921 atgaagagca gcaattaatt cactcttttg tcacccacac acagattcat gggcaaattg
    55981 gtcaccctta tatcaccagc ggggccgcct ccctgatcaa gcattttgta aaagattttt
    56041 atcatggcat tactgttacc tgccccggct tttacggtcc gcagggcagg atcctccggt
    56101 tgggcatccg taacccggaa ttgatcaacc gcctgaccga ttttcgtttc gggcaacatc
    56161 gcattaccaa tttcgaaatg gaaacttctg ccatttatgg tttagggaag ttgcttggac
    56221 ataattgcct tgccgtgaac gccattgttg ccaacagaat aaaaaaagag ttttcaaaag
    56281 atggcaaagc cgcagtagag aacctgatca aaaaattcat acagctattt tcagagaata
    56341 tttaatgcaa ttccactttt ataaatacca gggaacaggc aatgatttta ttattgctga
    56401 taacaggaag aatgaatatt cttcacttac gaccggacag atccatcatc tgtgcgaccg
    56461 ccgctttggc attggtgcgg atggattaat gctgctcaat gaaaaagaag gctatgattt
    56521 tgagatgaaa tattataatg ctgatggcaa agagggaacg atgtgtggca atggcggacg
    56581 ttgtatcacc aaatttgctt atcacctggg tattcaccgg gaagtatatc atttcctggc
    56641 tgcagacggg ccgcacgaag ccgaaattga tatgaatggt atcgtttcat taaaaatgaa
    56701 aaatgttgat gtaatcaaaa aattccaggg tgacctggtt ttgaatacag gctctcctca
    56761 ttatataaaa atggtacgca acgtaatgga tattgatgta tacaaaaagg gccatgacat
    56821 acgatacagc agggaatttt cggaggaagg gataaacgtt aattttgttg aacagactga
    56881 aaatccaaat aaaataatag tccgcaccta tgaaagaggt gtggaagatg aaacgtactc
    56941 ctgcggaacc ggagtaactg cagctgccct tgtttcctat cacaatgaaa atgggttcaa
    57001 tgatattgaa gtaaaaacac tgggcggcac tctcactgta gaatttgaca ggtttgatga
    57061 tgagcgatat gaaaatattt ggctctgcgg cccggccgaa aaagtttacg agggtgcaat
    57121 tgaaataaaa taacttatcc tggtttatca gccaccccgt tttataatct ccaattaacg
    57181 aaaaagaatt aaggaccgat tctgaaaccg caagcttacc tttgcgccgg tttatgattc
    57241 agtattttaa aaatatcaac cagcagacgg tagaaattga caagccggaa atcggcacct
    57301 gggtcaatgt attacccccg ctcaaacagg aagagttttc tgagctttcc accgggctgg
    57361 atattcccat tgactttctt actgactcac tggacataga cgaacgcagc cgttttgaag
    57421 aagaagataa cgttaagctt atcgttatca aaacaccgac agaaaataat tcctttaacg
    57481 aaagtgatgc ctattatatc accatcccga tatgtatcat ccttacgcac aaccagatct
    57541 taacggttaa ttcatttgac aaccccgcca tcaaaaaatt tctgaatacg tttcagaacc
    57601 ggcatccgga taaaaagaac atgatggtgc tgaaagtgtt tgaaaaagtg gtccagaatt
    57661 acatggatca cctgaaagag atcaatcagc gaagaaacat ctttgaacaa aaattatatg
    57721 ccgccagccg aaatgaacat ctcctggaat tgatgcggat acaaaaaagt ctcgtttatt
    57781 ttgttaccgc tatccggtcc aatgaattgc tgtttatgaa actgcaacgc accaatttcc
    57841 tctccctgaa tgaagaagag aaagagttcc tggatgacct gatcgttgac aattcacagg
    57901 cgctggaaat ggccaatatt tacagtaata tcctcagcag tacgctggat gcctttgcgg
    57961 gtattatcgc caataaccag aacgaagtat taaaaagact ttctgtaatc accattgtat
    58021 tgactttacc tgtactggtt gccagtattt atggcatgaa cgttcccatc ccctaccagg
    58081 aatcaaaact ggctttttat atgccggttg gactatcgct gatcatctct ttattcatcg
    58141 gctggttttt tcttaagaaa aaaatattct aacccaattt atgaatcgat ttaatatcag
    58201 ggtttatggt ttactgatca acgaacaaaa acaagtattg gtaagtgacg aactgatacg
    58261 tggcagctat atcaccaaat ttcctggtgg cggcctggag tttggtgaag gaacccggaa
    58321 ttgtttgcag cgggaattca tggaagaaat gaacctgcag gttgaaatag gtgaccatat
    58381 ctacacaaca gattatttcc aactatcagc ctttaatcct gaacaccaga taatatccat
    58441 ttactatttc gccaaagcat tagaagaaat taaagtccca ctaagagaca agccttttga
    58501 ttttgatgaa gaacagttga aagtatatga agcaacaaaa gaaacagaaa catttcgttt
    58561 cgttgactgg catgaattct ccccggaaag tgtaacactg cctattgaca aaattgtagc
    58621 ctccatcatt aaagaaagat attaatcatc ccccattgca gctttcttca tagctgttgc
    58681 ctgttcctga cccaggtaac tttcaatccg gttatgaacc aaatagataa caggggttag
    58741 aataattgcc actatgaatt tgtaaatata attaacgacc cctactgcaa taaaaaggct
    58801 gaaaggccat acaaagtcat tgcctgcagc attcactctt gtccctacat aaaatgcaat
    58861 aaaaagaaca acaaaactgt cgatgaactg ggagataagt gtagagccgg tggcccttaa
    58921 ccagattctt ttctcaccgg tgaccttttt tattttatga aaaacaaaaa catctattaa
    58981 ctgtcccaga atgaatgcag tcagtgaacc aataatgata aaacctccct ggcccaaaac
    59041 actattgtaa gctttttcca tatcaggtac accgctcccc tgcttactgg tgatgaaaaa
    59101 atcagcaggt gtcagtttca tagccccgta aaaaactata aacgcaaaag cgatcaggcc
    59161 ggccgtcagg taagaaagaa acttcactcc cttcatccca taatattcgt tgataatatc
    59221 tgtcatgata aaaacaaccg gccataataa cacaccggcc gtcaggttca cggaaagatc
    59281 attgccaaat aaacgaatgt gcagcggtgg aaagcccaat gtcttctcca gggaaaatat
    59341 tttaaccccg atgatttctg caatcagcgc attggcaata aaaatacctg ccaggatgat
    59401 aaacaacctg gtcggtttac ttctgatgat agtatggatc attgagataa aatataaaat
    59461 aagctaaagc cagtaagaag aatatattgg atacaatcaa ccacaaagga acatattgcc
    59521 ttattctttt cttcaccaaa aacacagcga tataacaaag gttcaccacc ggaaaaagaa
    59581 tgatacccat gaaataacca ataatgatga cagtagattt caggttttca tcctttagcc
    59641 agtccgttac caataacgaa gctgaagcaa gaaaaaaaac acctgagata aaagccagtc
    59701 ttgataaaaa taaaagccaa cgcatcggga attttagccc aaaatagcat tattttgtca
    59761 ccctaataac gtattggata tgaacaatcc cctgctgaag aaaattcttc cacatgctat
    59821 cgctattctc cttttcctgg tggtttctgc cctgttttgt aaacctgccc ttgaagggaa
    59881 tgtgctgaac cagcatgata atgtcggatg gaaaggaatg gcacaaaatg cttttgagta
    59941 taaagagaag aatggtcact ttcccctgtg gaatcccaac cttttcagcg gaatgcccaa
    60001 ttaccaggtg gcgatggaag gaaaaagcat tttaccggat acagtgaaaa tactttccct
    60061 tggattgccc aaacccatga actttttctt tttagcctgt ctttgctttt atattctgtg
    60121 tcttgtgtta agggtgaacc cggttatcgg aatgttagga gcccttgcct attcattctc
    60181 tacgtacaac ccggtcattg ttgctgcagg acatgataca caaatgctgg caactgcatt
    60241 catgcctttg ctgatggcgg gcattataag tatctatgaa aaaaaatact ggctgggttt
    60301 tgcactcact gcatttggca cctatcagca aataagtgtg aaccacctgc aggttagtta
    60361 ttactttttc ctgatcgccg tattgataac cattggctac ctggtaaaat ggatcattgc
    60421 aaaagactgg aaacatattg gtactgccgg tgctctcacc attatagcag cgatcatagg
    60481 catagccgga aatgcgctgg tacttaaaac cacttctgag tacagcaaat tcaccatgcg
    60541 gggaggaaaa gacatttcga ttgaaggcga ttcagttaag gcagcaaaaa cttccgggct
    60601 cgacaccagt tatgcctttg aatatagtct aggaaatgcg gaagcactta ccctattaat
    60661 gcctgatgcg tttggaggtg ccagtagcaa catgagcagt gaagagtcgc atatcataga
    60721 taaattaact tcaagaggta taccggagag tgatgcacaa caactggcac aaagccttcc
    60781 gaggtactgg gggaaattat atacttccgg acctgcctac ctgggtgtga tcattttctt
    60841 attgggactg atcgggtttg ttattgttaa aacaccattg agatggggtt tattagcggc
    60901 aacaatactg ggtatactaa tggcatgggg aaaatattta cccggcttca atacatttct
    60961 tttcgaacat cttccgctgt acaataaatt tcgttccccg gcatttgcac aagtgatacc
    61021 gcaatttgca atgggagtaa ttgcagtact ggcattacac cagatactct tcagtgaaaa
    61081 aagtaaagag tcgctcaaaa atgatttcaa gaaaattcta tacacagctg gtggtttatt
    61141 cggggtgctt ggcattcttt atatagccat gaattatagt tccccgattg acggacagat
    61201 cattgctggc tataccgata aaaatggttc tgatgaaatg gcccgattga tcgtctccgg
    61261 gctgaaagaa gacaggaaag ccatgttcgg cggtcaaatt ttgagagcac ttgcttttgc
    61321 catcctggct ttgggaataa tctggctgta tgcaaaagac aaactcagtt cctttgttgc
    61381 ggctattgtg ttactcatca tcagcaccct tgagatcacc atagtcagta aaaaatattt
    61441 aagtgatgag agctatattt ctgctgatga ttatgcgaat tcaaattttg ctccttcctc
    61501 cattgatcag caaatactgc aggataaaga tcccaattac agggtattta atatggtagg
    61561 tacttcatcc ggaagtactt tcagcgagtc aaagacatct tactttcata aatcagtagg
    61621 aggttatcat ccggccaagc tgagaattta ccaggatatc attgaacgtt acttgtcagg
    61681 caggccatcc cctatggtat tgaatatgct taatgcgagg tatattgttg tccaggatcc
    61741 gcagaacggg caacccgggc tgatcactaa tcctgatgca ttcggtaatt gctggctggt
    61801 aaaaaatgtt agactggtag agaaccgggt ggaagcgatc aatgccttgg gtacaaccaa
    61861 cctgaaagac actgccattg tagaaaaatc gcaactaacc aacctggctc aaccacagtt
    61921 agactccgca tcctctatca aaatgacaaa atttgataat gatgctatag aatacgaggc
    61981 aaattgtaat ggaccacaat ttgcagtatt cagcgagatc tattatccaa aaggatggaa
    62041 tgcttatgtt gatggcaaga aagtggacta ctataatacc aactatattc ttcgtggttt
    62101 gccattaccg gcaggaaaac ataccgtgaa atttgtcttt gaacctgaat cggtaaaaca
    62161 gggcaggtct atcatgttca ttgcctccat acttatcctt gtaatactac tgggaggatt
    62221 attcatagcc tggaagcaaa gtaaaaaaac aagctgacgg ggctaatgaa aaaagtactg
    62281 gtgatagctc cttaccccta cctgccttat ttttcaggtg ggcagaaatt tattgcccag
    62341 ttccttgaat attttgccaa cgaggttaac cttaccgtga tcagtgtgca ggaaaatgat
    62401 tttagcctcg ttaaaaatta ccggacaata ccattgctaa aaaaatcatt ctaccgctat
    62461 attgacctaa gcctggtaaa aaagatcagc catgaaataa agctgaataa atacgatgcc
    62521 ataatctggg aacaccctta ttatgcatgg ttggcgttca gtgttaaaaa gcgaacaggt
    62581 atccaaacga ttattcatac acacaatata gaacaccagc ggttccgctc taccggacga
    62641 tggtggtggc ccctgttaaa actgtatgaa aaatggagtt ttaaaaaagc tgacaaactt
    62701 tttttcatta ccccggagga taaagatttc gctattaatt cctggaaaac agatcctgcc
    62761 aaatgtatcc atcttccttt tgggatcact atcaaagaac acccttcaga taatacttcc
    62821 agcaaagaag caataagaca gcagcatgct attgcaccgg atgaaaaaat aatcctcttt
    62881 aatggcctgt taaggtataa gcccaacctg gatgcattaa aatatatttt agaaagtatc
    62941 aatccactgt tgctggcagc cccttccttc cgctataaga tcattatttg tggtaaagat
    63001 atacctgcgg aactgaacgc attaaccagt tacactgata agaatatcat ctttgccggt
    63061 tttgttgaga atatcgaaac ctattttaaa gcagctgata ttttcctgaa ccctgtacaa
    63121 agcggcggcg gtattaaaac aaaaatggtg gaagctattg cgtacggagc cactgtaata
    63181 gccacagaaa gcggagcggc cgggttagat aaaaaagtat gcgggaacaa gctggtaacg
    63241 gttcctgaca atgactggga aacttttgcc gaaaaggtgc tggagagcag taaaacttca
    63301 accaacactc ctgccgttta ctacgaaaca tattactggg gaaatatcgt aaagaaaata
    63361 gcctcg
//
LOCUS       DAORTI010000006        61950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_228_length_61950_cov_6.842120, whole genome shotgun sequence.
ACCESSION   DAORTI010000006 DAORTI010000000
VERSION     DAORTI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 61950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61950
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228_length_61950_cov_6.842120"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="rfbC"
                     /locus_tag="PLO70_01725"
     CDS             <1..272
                     /gene="rfbC"
                     /locus_tag="PLO70_01725"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQZ73202.1"
                     /translation="FSIVLSAKNKRQLLVPKGFAHGFSVLSKNAEVLYKCDGFYNKES
                     EGGIVYNDPALSIDWRIPAADAIISDKDKVLPVLAACNNSFVFNG"
     gene            272..1135
                     /gene="rfbD"
                     /locus_tag="PLO70_01730"
     CDS             272..1135
                     /gene="rfbD"
                     /locus_tag="PLO70_01730"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQZ73203.1"
                     /translation="MESKQKILITGSNGQLGKELKRLENSFPQFNFIFLSREDLPIHH
                     YELVRNFFKGYQPQFLINCAAYTAVDRAESEKELAFQVNGESVGVLAAVCKEYQTKFI
                     HISTDYVFDGTATVPYKEDSLTNPQSVYGASKLEGEKEALRFNPDSIIIRTSWVYSEF
                     GKNFVKTMLKLMNEREELNVVSDQFGSPTYAADLAEAILQIISSGQWHPGIYHYSNEG
                     KISWYEFAVAIKALSGNVCKVNPIPTSQYPTPAKRPAYSVLDKTKIQSAFSVRIKDWK
                     TSLETCLSRLK"
     gene            complement(1163..1233)
                     /locus_tag="PLO70_01735"
     tRNA            complement(1163..1233)
                     /locus_tag="PLO70_01735"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1199..1201),aa:Gln,seq:ttg)
     gene            complement(1311..3959)
                     /gene="cphA"
                     /locus_tag="PLO70_01740"
     CDS             complement(1311..3959)
                     /gene="cphA"
                     /locus_tag="PLO70_01740"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQZ73204.1"
                     /translation="MQILEIKVLKGPNYWSVRRTKLIQMKLDLEEKENMPTNKIPGFR
                     ERLEKLLPSLYEHRCSVGKPGGFFERVDEGTWMGHVVEHIALEIQTLAGMDTGFGRTR
                     TPEGEKEGIYYVVFSYMEEDAGVYAAKAAVRIAQALISGVDYDLTEDIQQMRVIREDT
                     RLGPSTGCIVDEAAKRGIPYIRLNKASLVQLGYGVNQKRIRATIASTTSNIAVDIACD
                     KEETKMLLEAAEIPVPRGTVVRTEEGLDQAIEKFGYPLVIKPIDGNHGKGNTTNITNR
                     TQAIKAFDAAKAYSRSVIVERFITGYDFRCLVINYKFICAALRTPASVVGDGVHDIRY
                     LIEEVNKDPRRGYGHEKVLTQITVDQFTQKMLDDTGITMETVPQKGERVLLKPTANLS
                     TGGTSTDITDEVHPANIFMFERIARIIGLDICGIDVMAMDLKTPVAENGGAILEVNAA
                     PGFRMHIEPAEGLPRNVAEPVVDMLFPKGSAGRIPIIAITGTNGKTTTTRLTAHIAKS
                     AGKKVGYTTSDGVYIQNQLMMKGDCTGPVSSTFVLKDPTVDFAVLECARGGILKAGLA
                     FQHCDVAVVTNVAADHIGLGGIESVEQMAKVKAVVPETVFPHGYAVLNADDDLVYKMK
                     KDLKCNIALFSMDENNPRIKEHCANHGLACVYENGYVTIMKGNWKIRVLAARDIPLTY
                     EAKAVHNINNCLPAVLAAYLYRDITIDDIRIGLQTFIPSESLTPGRLNFFHFKHFTML
                     ADFAHNPHGLRLLCDFVSKLDYPTKIGVISGTGDRRDEDIRELGEISASHFDEIIIRC
                     DKNLRGRTADEIIDLLKEGINKVNPDIPVLTIPNEDKALDYIYENAKPGALYTIMCDV
                     VARALDKIKELKVREEKEGQKTAQTA"
     gene            4161..5456
                     /locus_tag="PLO70_01745"
     CDS             4161..5456
                     /locus_tag="PLO70_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQZ73205.1"
                     /translation="MSATNRRGFLQKAGMFSATAFLSSLGQPVWSRNLENVLRNAEGI
                     SPEDLAAEEDFWYYIQQAFTVSPSLINLNNGGVSPAPKTVQEAMKRYYDLCNEAPSYY
                     MWRILDQGREPLRKNLAKLGGCSPEEIAINRNSSEGLETVIFGLQLREGDEVVAAKQD
                     YPNMVNAYKQREIRDGIKIKWISLELPSEDENYLVQQYVNAFTDKTKVVHITHVINWN
                     GQILPVKKIAQEAHKRGIEVVVDGAHSFAHFDFKIPDLDADYFAASLHKWLYAPIGSG
                     LLYVRKEKIKDIYPMFAVDKPLKDDIRKFEALGTRPFYIEQAIGKAIEFHEMIGIERK
                     EKRLHFLKNYWMEKVKNIPKVKLNTSLHPKWGCAIGNVGIEGKKPNELDSFLLDKYKV
                     HTVAITWENIVGVRVTPNVYTTTKNLDVLIEGISAFAKS"
     gene            5488..6810
                     /locus_tag="PLO70_01750"
     CDS             5488..6810
                     /locus_tag="PLO70_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQZ73206.1"
                     /translation="MSIPLTNRKINLLQATSINMIDMVGIGPFVVMPFVVAQFNNGLF
                     IWAWIFGAFTAFMDAMIWSELGAKYPLAGGTYNFHRIAFGERGGKLMSFLFVWQTSIQ
                     APLVVASASIGFAQYLTYIVPLDIWQQKIVSGGLVMLVFILLYRKIETIGKISVVMGT
                     IVVLTIIWIIISGLLNQQHPVKLIPTGKESFFTYAFWAAVGQASVKTVYAYLGYYNVC
                     HLGGEIKNPGKNIPRSIFISVFGIATLYLLMNISVMGVMPWELVKADDKYLVSSFMQQ
                     LYGTKAGVLVTILILCIAFSSLFAVVLGYSRVPYAAAVDGNFFKPFAKLHPTKNFPYI
                     SLIVLCTLGFIFSLLFKLAEVISSILAMRIVIQFIGQAVGVVMLRKRFGEKDLPFKMM
                     LFPLPVILSIVIWAFLFVSTGWFALWGSLIALCGVGVYFFTSRLKKSE"
     gene            6902..7798
                     /locus_tag="PLO70_01755"
     CDS             6902..7798
                     /locus_tag="PLO70_01755"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQZ73207.1"
                     /translation="MSNPKGKLIAIGGAEHKGTELEADGFHRNNLNFFELGILRRVVE
                     EAGGLNARIEVITTASMIPYEVGENYLNAFGKIGCTNIGLLHIRTRQDAMNEEYVDRI
                     RHCDAVMFSGGNQLRLSATDGGTEFLTILKKRYKEEENFLIAGTSAGAMAMSNTMIYE
                     GNATRAHLKGEVKITTGLGFMNNIIIDSHFEKRGRFGRLVQAVAANPGCIGVGLGEDT
                     GMLITEGNKMEAIGSGLVVIIDGHDILHSNIADIPDGNPISIENLKVHFCEKGNGYLI
                     KERRYLPEASDGSVIKKQVDVE"
     gene            7947..9878
                     /locus_tag="PLO70_01760"
     CDS             7947..9878
                     /locus_tag="PLO70_01760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQZ73208.1"
                     /translation="MKLFQQRLLAVAALAILVFAACKSKTVKEEKPPVIPVENFFKNP
                     ENFGWRISPDGEYISYLSPYKGHTNVFVRKIADSVAIPVTSDSVRNIFDYQWKGNRIL
                     YLQDIGGDENFQLFSVGLDGKNLKPLTPFAKVRTSILNDLRYVPGKEKEVMIQMNKRD
                     PRFFDPYSINIETGEMKVLFQNDKNYDGWFTDHTGLIRIATKTDGVNTTYYHRATEDD
                     PFDSLLTTTYKDNFGIQLFTFDNKNLYVASNIGRDKTTILEYDLAARKEVKEIYSNPD
                     YDVDGLSWSPKRKVLESADFTSWKREQKFLDKEAETEYNKMKAKFEGYEVGLYGSNDA
                     EDKFVVWTGNDKLAAKYYFYDKKSGETKFLSDAFPWLKEENMASVKPVEYKSRDGLTI
                     HGYLTLPKGIEAKNLPVVINPHGGPWARDSWGYNGETQFLANRGYAVLQMNFRGSTGY
                     GKEFWLKGAKEWGKKMQDDITDGVQWLITEGIADPKRVAIYGGSYGGYATLAGVAFSP
                     DLYCCAVDYVGVSNMFTFMKTIPPYWEPYKAMFYELVGDPVKDSALLAEVSPAFHADK
                     IKAPLFIAQGANDPRVNKAESDQMVEALKKRGIEVEYMVKDDEGHGFANEKNSIDFYK
                     AMESFFARHLSASKEEVKK"
     gene            9961..10641
                     /locus_tag="PLO70_01765"
     CDS             9961..10641
                     /locus_tag="PLO70_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQZ73209.1"
                     /translation="MKKLLSLVILVVVSMAFTPQKKTKVIFFGDSITELGVKKDKYVG
                     YIVRMDSMLKAVKKDDQYELIGSGISANKVYDLYLRLEEDVLGKNPDIVVIYVGVNDV
                     WHKTLLGTGTDPDKFEKFYNAILRKLKERNIKAILCTPAVVGEKTDMSNPLDGDLNRY
                     SNIIRDIAKKNDLPLVDLRQKFLDYNNTNNPENKEKDILTYDRVHLNAKGNQLVADAM
                     WKAIKEIK"
     gene            complement(10634..11911)
                     /gene="tyrS"
                     /locus_tag="PLO70_01770"
     CDS             complement(10634..11911)
                     /gene="tyrS"
                     /locus_tag="PLO70_01770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQZ73210.1"
                     /translation="MNLIEELRWRGMIQDIMPGTEEHLNKEMTTGYIGFDPTADSLHI
                     GSLVPILLLVHFQKAGHKPIALVGGATGMVGDPSGKSEERNLLSEEALQKNVAGVKAQ
                     LEKFLDFNPSLQNAAEMANNYDWFKSISFIDFLRDAGKHITVNYMMAKDSVKKRIEGE
                     TGISYTEFAYQLMQGYDFYWLYQHKNCKLQMGGSDQWGNMTTGTELIRRKCGGEAFVF
                     TNPLITKADGGKFGKTESGNVWLDANRTSPFQFYQFWLNASDTDAEKWIKIFTFLSKD
                     AIEMLVHEHKMNPAARVLQKTLAQEITSFVHGKDEYEKAIETTHKLFANQNAPAESLS
                     AEDLENLEGVVKSNFSKEKITAGIDIVSFLAETAIFDSKGNARKNVQGGGVSINRKKV
                     EAADFKIDPSLLLHDKYILVQKGKKNYYLVKVI"
     gene            12151..13260
                     /locus_tag="PLO70_01775"
     CDS             12151..13260
                     /locus_tag="PLO70_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQZ73211.1"
                     /translation="MKTNRLYLTLIAFFLFLTSNAQFRKYSNEFLNIGAGARGLAMGS
                     AQVASVSDGTSGYWNPAGLVSIKDHPQINLMHAEYFAGIGKYDYASIAFPTANNKRTI
                     GITGLRFAVDDIMNTLFLVEPDGSINYNNIQSFSSADYAFIFSFAQKIKETERKKVNF
                     GVNAKVIHRSVGKFAKAWGFGLDAGLQIHSGKWRFGVAGRDITSTFNAWSFTFTEREK
                     EVLYLTNNEIPVKSTELTAPRLVLGIGREFKLGQKSSLLAEANIDLTFDGQRNTLISA
                     DPVSADPKLGLELNVNNVFFLRGGINNFQKALSDGDTLNQKKVWIYQPSAGAGFRIQN
                     VAVDYAFSNLANQSNPLFTHVFSLRLNLVNTRNKN"
     gene            13293..18401
                     /locus_tag="PLO70_01780"
     CDS             13293..18401
                     /locus_tag="PLO70_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQZ73212.1"
                     /translation="MKKFLLPLLLCTGLLAQSQDYNNEWINYNLTYYKFKIGATGLYR
                     ISQPTLATLGIGNTPAEHFQLWRNGQQIPVYTTVQTGPMGVSDYIEFWGEMNDGKPDN
                     PLYRNPDFQLNDKWSLETDTVSFFLAINPGGNNLRLVPTNNTLPSALPAEPFFIHTAG
                     KYYREKLNPGYAAVVGEYVYSSSYDQGEGWTSNDLGTGVTRPENLSNLYPYTGAGAPA
                     PLIKVNATGNALNPRQFEIKINGTVLSTQTMDFFDYKKVQAVFSASLIGGGAANVEVK
                     NLCTLPNDRMSIAQTELVYARQFDFGGANNFSFELPANTAGNYLEISGFNHGSIGPVF
                     YDLTNGKRYVGDVTTPSLVKIALEPSSVNRKLLLVSQHSSFPVTAVTLLQQRNFVNYA
                     AASNQGNYLIISHPALMNGSGGSNPVEDYKNYRSSAAGGSHNAKIYLVDELFDQFGYG
                     IKKNPLALRNFIRWTRATYSSPVKNVLLLGKGLNYVQYRAYESNPDVERLSFIPTFGY
                     PASDNMLVAEPGLDGIPRVPVGRISAINADEVAVYLAKVIQYEQQQAFSSPLIADKAW
                     MKNVAHVIGAADGSLGAILTADMNRYKSIISDTLFGADVHTFSKVSSAPVEQASSEKL
                     YQLFQEGIGLMTYFGHSSATTLEFNLDNPDQYNNPGKYPVTIVMGCNAGNFFNFNTLR
                     FITKETLSEKFVLANQRGSIAFIASTHFGIVHYLDIFNTRTMTATSVSHYGKTLGEIL
                     IESITQVYNLTTQNDYYARFHCEQTTLHGDPALKFDASTPKPDYAIEEQLVKVNPSFI
                     SVAETHFKVDAKFMNLGKAINKNIVVEVKRTYPNLTTEVIRRDTIPGIRFIDSILYAI
                     EIVPTRDKGLNKISICIDADNVVDELYETNNCITKDVFIYEDEARPVYPYTYAIINKQ
                     DIKLVASTANPFSGIKQYTMEMDTTEFFNSPAKITRTISSAGGLLEFTPGVAFTDSTV
                     YHWRVAPVPTSGQPSWNKSSFVYLNSTGPNSSDLGYNQSDFYQHTKSEYERLKIDSAS
                     RSLKFGPVVNNLFIRLGTWITSGATQEASLAISINGVSAIRLMCWFSSLVFNVFDPVS
                     FRPWQNQRIVANNYPTSLGEGLYGSTDNNCFGSTKIFNFEYRYTDTASRRKMMDFMRD
                     VIPDGAYVAVRNFTLDPAFGFPNAWAADWAADEAIHGPGQSLYHYLKNAGLSSIDSFY
                     RTRPWALVYKKNDPSFTPKWIMGDGMFDNPTLSVDCSTADTVGHITSPVFGPARAWKQ
                     LKWRGSSEPAGDTATVDVIGVRADGSEQTLFNGLTTAQQDFDVSSIDPNVYPYVKLKM
                     RTEDNINFTPYQLRYWRITYVPVPEGAIAPNLHLVVKDTLEVGEPYNFKVGFKNVSEV
                     AFDSLKVKMIITDRNNVPHIIPIPRRRPLPINDTLQIGSTVNTAAIPGANTVYIEANP
                     DNDQLEQYHFNNFAYRSLYIKPDSLNPLLDVTFDGMHILNRDIVSSKPDILVKLKDEA
                     KWMILDDTSLLTLQVRYPNGTLRRFYFNNDTLRFNPAGQAPNPDNTATINFKPYFQLD
                     GEYEMIITGKDKSENSAGSIEYRVLFEVINKPMISNMLNYPNPFTTSTSFVFTITGNE
                     VPQNIKIEIMTVTGKIVREITKDELGPLRIGRNITEFKWDGTDEYGQKLANGIYLYRV
                     VTNLNGKALDKYKSQNDNTDKYFNNGYGKMYLMR"
     gene            18484..19317
                     /locus_tag="PLO70_01785"
     CDS             18484..19317
                     /locus_tag="PLO70_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73213.1"
                     /translation="MLLVKTWIKRNFSEWIYYRNKRPGRKSETDNWLNDLCRRQISKL
                     SDSSYNNFTYHGEDGILLYLLRQIKDVPNVFVDIGAGDCIKSNCANLAVHYGWDGVFI
                     DKNDKQIAIGKRFYRTMIDRGRDIRFLEQMIIPENVNNLLENSGIGGEIGLLSIDVDG
                     NDYWIWEAIKVIKPRIVVIEAKVEFGTRNVIVPYGDHNHYSVDKMYNGASVEAIRKLG
                     KMKGYKMAGSNRQGYNLFFVRQEENITEESVDSVLSDPETIQSFYPESFYKKHNFITV
                     N"
     gene            19323..21185
                     /gene="hscA"
                     /locus_tag="PLO70_01790"
     CDS             19323..21185
                     /gene="hscA"
                     /locus_tag="PLO70_01790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HQZ73214.1"
                     /translation="MAKIGINIATGSLQKQEMIVGIDLGTTNSLVAIIHPETGQPVVL
                     KEHDGMALVPSIVHFGNEGQIAVGDEAKKFLIAEPENTIFSVKRLMGKSYSDVKEHSS
                     FFSYKIIDDNTESLVKIQVGSRFYSPVELSSFILKELKARAEHILKTPVTKAVITVPA
                     YFSDAQRQATRDAGKLAGLDVLRIVNEPTAASLAYGMGFSREETKTIAVYDLGGGTFD
                     ISILRINGGIFEVLSTNGDTYLGGDDFDREIVKYWATELGITIEELGVHKNLAQELRL
                     LAEEAKKLLTTSDNFKGKVAGQEVKLTKKQFENLILPLVLRTIQSCKNALKDADLTIG
                     DIDEVVMVGGSTRVPLVKQNVSIFFGKNVHDSLNPDEVVALGAAVQADILAGNNKDLL
                     LLDITPLSLGIETMGGLMDVLIPRNSKIPSKAARQYTTQKDGQGSMRIAVFQGERDLV
                     RDNRKLAEFNLTGIPGMPAGLPKVEVSFLVNADGILVVKAKELRSGVEQSIEVKPQYG
                     LTDAEVEKMLMDSIAHAREDIQVRALVEAQTEAKQIIDTTTQFILKNSNFLSDEEIQK
                     TRQAMDALSVLTTKGAKDEIQAGIEKLNEISRPYAERLMDEAISKAIKGKSITE"
     gene            complement(21175..22755)
                     /locus_tag="PLO70_01795"
     CDS             complement(21175..22755)
                     /locus_tag="PLO70_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73215.1"
                     /translation="MNRNRMILLSLLLALPVILFLLHHYFYHSNDLQPTGFTVDENIL
                     YMSYARQYVDQDSFSIFYSNPFDGNPQSAKIYFQPINFLFAAAIKAGLDPGLTFSLFG
                     LIMAIFCIYTGIQIIQHLLNGQRNQTLISLLFTWGGGLTAIAGFAAAFFLSDQQPPSW
                     LDSIHIADPANGWWGLNWGRNLFIPLEAYYHFLFLLNIYLILKRKWSAAIVTAFFLFI
                     SHPFTGIEYLLIMNGWIFIEKLLFKNKEIPIYYWAGITCITFFHAWYYLIYLNSFPEH
                     QQLYSQYSAGWTYSLRIAIPAYLIVIILSVLATRINKTTKILSHSHQRLFLCWAVIAF
                     LLSKHEWFIRPMQPIHFTRGYVWAGLFLFSLPALLWLINESNKSQLRKWLFKGFIILF
                     LLDNILWTVNILRKKDTSEWEGHITKDTKEVLEFLKNNKTTNDLLIGNAVLLNYISNA
                     YCSANSWVSHPYNTPNRERRLEQEKNFLKTGSKPPEWKNRRIILIIDKVSNDIIIIKP
                     DLFKERIFENSRYIIFIL"
     gene            complement(22755..24950)
                     /locus_tag="PLO70_01800"
     CDS             complement(22755..24950)
                     /locus_tag="PLO70_01800"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQZ73216.1"
                     /translation="MNITGIFQQKHDQYRNYLFLLIICLLTYWPLTFNIFSVKNDAIH
                     YFLPYRFNISEAIRNGEWPFWSPYIYLGNPIYGDMQSGVWNPFVWLFSCISRYNITLF
                     HWENLLYIFLAGAGMYKLVNRLTVHTHAAFLIAVSYMLSGFMLSGQLINWLASAAFIP
                     FVVHYYTLLLKTARNGSAIKTGIALFFLFTAGYPSFFITTCYILLFLFIITIVRKLIL
                     KESETAKWSLFYLQHLLLILVFVGLSLPAIVSYIDLLPYYQRGSGVSYTEAVINSFEW
                     QHFLSVLFPSSISATDISSSTDISFRNVYTGIFPLLILFTFPPRPNRKNILLIGLALF
                     AVLFSLGDLTPLRKLCYDYLPLMNTFRHPSQVRLFFIFAVLLLAAPGLSELIKNYSNT
                     QIISTLNFPAIIITGIILLITIIAIVQSGIPVFFKPINNNAITTSIKDIIDNISLSDA
                     VIINGIIQLAFIASFALWIRKTRRKIGVFSLLWIVNLFIMSQFSLPATFVGKTAPKEI
                     NALINNSPAGYPPEGLENTISENSKSATDDFNKIALLYFYNKKIGISKTVNSPAFLHQ
                     QEQFINNELLYNYIASFPVVYIADQTLSLKDSNKLITKNSCNYAITDTVLVTNRICDK
                     PIKAQIIKLSANKFEIETEMNRQGFLVLTQNYNHNWKVTVNKRPAVIYKTNLSFIGTL
                     LATGKHKIIFEFFPANTEKAIWIMCSTILTLLLLGTVSLVRQSKNPNAG"
     gene            complement(25007..25882)
                     /locus_tag="PLO70_01805"
     CDS             complement(25007..25882)
                     /locus_tag="PLO70_01805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73217.1"
                     /translation="MAKDYEYHSVKEDFTYYHCTDCGTIFIHPVPLSQLKEIYPSNYY
                     SFVTVSKNIVVKIKEWLDKRLFKKILSQLNSAKIDVLDVGGGTGWILDVLKKTENRID
                     FTQIADIDEKASIVAEQNGHAYFKGTIEEFNTDKRFHLILMLNLIEHVANPLAVLQKA
                     ETLLHPGGIIMIKTPNTDSLDARIFRRTYWGGLHSPRHWVIFSEKSFRILLQSTRLNI
                     KKLNYTQGAPFWAFSILVALHRKRIIHTSAQNPIIFHWLFAPVSALFALFDFIRRPFA
                     KTSQMFILLTNEKNQ"
     gene            complement(25939..26898)
                     /locus_tag="PLO70_01810"
     CDS             complement(25939..26898)
                     /locus_tag="PLO70_01810"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQZ73218.1"
                     /translation="MQNNLEGISFDEVTDKTGISYAVFRTTLLPRYTKVISDIIKGYF
                     FLLLISCLVILLNQTYPSLWWVYISAGSILIGYIAAYLALFIHEAGHYNVHPDKKIND
                     SLATFFLCLPFGLSMKAYRKIHWQHHLYLGTPQDTETSYFNPLTKLFILETLTGIHLL
                     RTMLKKENNNVLTKEQKKESSIMLIAGFLLHAIILALTFLTGHWTFAITWILGFGFFF
                     PFFATIRQILEHRDVLADHATNFNKIQHGKISRLFIHTILSSSFGAAGFTRHMIHHWD
                     PQISYTRLKDIEDFLLYNKKTAEIIHSSKTSYSSVLKKLLAAP"
     gene            complement(26931..27644)
                     /locus_tag="PLO70_01815"
     CDS             complement(26931..27644)
                     /locus_tag="PLO70_01815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73219.1"
                     /translation="MTDSFKYQGGELVLFQYAKNWKEYFSQQITPYINGNVLETGAGI
                     GATTLLLNNGCYAKWLLLEPDKEMSEILDKKISEKELPANCKLQTGTIDQLTEIFDTI
                     LYIDVLEHIESDKEEIKKAAALLSNGGHLIVLSPAFQFLYNPFDKAIGHYRRYNKKML
                     RQLTPATLKMLNCRYYDTTGYFAALVNKFLLRQKYPTIKQVLFWDRWIIPASRITDKL
                     LFNSFGKSIIAVWEKEQPQ"
     gene            complement(27648..28589)
                     /locus_tag="PLO70_01820"
     CDS             complement(27648..28589)
                     /locus_tag="PLO70_01820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ73220.1"
                     /translation="MAEQIIILIPVYNDEKSVNKLLNELSVSLKDLNNYNFSVLIVND
                     GSTESINISKDSTFPGTILQLQRNIGHQKTIAIGLAYIKENLSCDKVLVMDSDGEDRP
                     EDAVKLLEEADKNPGKIIFAHRKSRQESNKFQLFYRIYKMVFHLFTGKKITFGNFLVM
                     PKLLLDKVVFYSEIWSHIAGGIIKTGLPYILVNTHRGKRYDGSSKMSFTSLLIHGLGA
                     IAVFIEIIASRLLIFSLLLIGISLIVILGLLGIKTFTDRAIPGWTSTVISAMLIILLQ
                     SFLLSLFTIFLYLSSQSQRKFIPAKHYKDYAGAVETV"
     gene            complement(28644..29729)
                     /locus_tag="PLO70_01825"
     CDS             complement(28644..29729)
                     /locus_tag="PLO70_01825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQZ73221.1"
                     /translation="MNPENPNRQYYPALDGLRGLAILLVVVYHNFGFIDYFFFGWLGV
                     DLFFVLSGFLITDILLKTVGTKNYLKNFYIRRILRIFPLYYLFLIVFLLIIPRLNIEL
                     NIKYYTDNQIWLWTYLQNWLYIFKDPDQTNALNHLWSLAVEEQFYLLWPLVIMIIRKP
                     KYLLLVISSLLAGVLFLRLWIWNNQVADLAYFNLYTFTRIDGICIGCTIALLHRINSN
                     FLKTYTTEIVLFFSVLNFAFFFINRSYHFSFPYLALVGYTTIAMIFGLLVNEAVSGKT
                     KLINLLFNIPLLKFFGRISYGFYIFHWPIYILLAPSLFPVLSKQVSGSLLPFTVSLLA
                     TLAAIFISWISFQYFEKYFLKLKDTFI"
     gene            complement(29752..30342)
                     /locus_tag="PLO70_01830"
     CDS             complement(29752..30342)
                     /locus_tag="PLO70_01830"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQZ73222.1"
                     /translation="MFTGIIECTGTVKEVISNGSNRTFWVESPISNQLKIDQSMSHNG
                     ACLTVEEISNHRHRITAIDETIQKTNLGQWQEGSLVNLERCLQLNDRIDGHLVQGHVD
                     ATGTCISKLEKEGSWEFGFAFPKKFAALVIEKGSICLNGISLTVFNVKKKTFNVAIIP
                     YTFEHTNIRNINIGDQVNLEFDMIGKYLLRRLSLKD"
     gene            30440..30637
                     /gene="rpsU"
                     /locus_tag="PLO70_01835"
     CDS             30440..30637
                     /gene="rpsU"
                     /locus_tag="PLO70_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQZ73223.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKSKVLLQLRERQSFTKPSVK
                     RRGEVLKAIYKQNLASGKIES"
     gene            30709..31611
                     /locus_tag="PLO70_01840"
     CDS             30709..31611
                     /locus_tag="PLO70_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQZ73224.1"
                     /translation="MPVPARQRIQSFLDYLKFERRYSPHTLTSYTTDLIDFFDYLESH
                     FDIVDVKAINSGFIRSWLAGLKEKGLTSKSVNRKISSLKSFFKYLLKTGEIENSPMNN
                     VISPKISKRLPVFMKEEDTKKLVLALNQSAEDWKSLNAKMLITIFYATGMRLSELINL
                     KEKQIDFSRGHIKVLGKGNKERIIPVSKEVIEVIGEYQQLKRRDFVGTEDYLLITEKG
                     NKLYPRYAWQLVNQVLGQASTLDKKSPHVLRHTFATHLMNNGADLNAVKELLGHSSLA
                     STQVYTHNTIAKLKDIHKKAHPKA"
     gene            31767..32066
                     /gene="raiA"
                     /locus_tag="PLO70_01845"
     CDS             31767..32066
                     /gene="raiA"
                     /locus_tag="PLO70_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQZ73225.1"
                     /translation="MNVKIQTVHFNADSKLKEYVNRKLEKLTVFSDRIIEVNVFLKLD
                     NVAHSIKDKIAEIRVHVPRHNFFVKATSKSFEESFESAFDSLVSQIKRKKEKQAA"
     gene            32238..32310
                     /locus_tag="PLO70_01850"
     tRNA            32238..32310
                     /locus_tag="PLO70_01850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32271..32273,aa:Thr,seq:tgt)
     gene            32317..32399
                     /locus_tag="PLO70_01855"
     tRNA            32317..32399
                     /locus_tag="PLO70_01855"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32351..32353,aa:Tyr,seq:gta)
     gene            32457..32529
                     /locus_tag="PLO70_01860"
     tRNA            32457..32529
                     /locus_tag="PLO70_01860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32490..32492,aa:Gly,seq:tcc)
     gene            32572..32643
                     /locus_tag="PLO70_01865"
     tRNA            32572..32643
                     /locus_tag="PLO70_01865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32604..32606,aa:Thr,seq:ggt)
     gene            32792..33979
                     /gene="tuf"
                     /locus_tag="PLO70_01870"
     CDS             32792..33979
                     /gene="tuf"
                     /locus_tag="PLO70_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQZ73226.1"
                     /translation="MSKETFKRDKPHVNVGTIGHIDHGKTTLTAAITTILASKGMATA
                     KKYDEIDAAPEEKERGITINTAHVEYQTANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTKEHILLARQVGVPKIVVFMNKVDLVDDPELLELVEMEIRDLLTF
                     YGFDGANTPIIKGSAIKALEGEAEAVKQVEELMEAVDTYIPLPPRPVDQPFLMSVEDV
                     FSITGRGTVATGRIERGRIKVGEPVEIVGLIAEPLTSVVTGVEMFRKLLDEGEAGDNA
                     GLLLRGIEKTQIRRGMVIVKPGSITPHTEFKAEVYVLSKEEGGRHTPFFQKYRPQFYF
                     RTTDVTGEVELNAGTEMVMPGDNTSLTVKLIQPIAMEKGLKFAIREGGRTVGAGQVTE
                     ILK"
     gene            34052..34122
                     /locus_tag="PLO70_01875"
     tRNA            34052..34122
                     /locus_tag="PLO70_01875"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34084..34086,aa:Trp,seq:cca)
     gene            34136..34330
                     /gene="secE"
                     /locus_tag="PLO70_01880"
     CDS             34136..34330
                     /gene="secE"
                     /locus_tag="PLO70_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQZ73227.1"
                     /translation="MNKITTYFKESYKELADKVTWPTWPQLQQSTMIVLAATIVFTFI
                     VLAMDFVAGNGLKFIYKQLF"
     gene            34330..34908
                     /gene="nusG"
                     /locus_tag="PLO70_01885"
     CDS             34330..34908
                     /gene="nusG"
                     /locus_tag="PLO70_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQZ73228.1"
                     /translation="MEATTNTDTQQQETKWYVLRVVSGKEKKVKEYLDKEISRGGWSE
                     IVKQVFLPVEKVYKVASGKKVMRERNYYPGYVMIEITEGKLSDDLRDVIKNTNSVIHF
                     LGKENPIALRKAEVNKMLGKMDEMAEAGGVSMSEPFIVGETIKIIEGPFNDFNGVIEE
                     VNDEKKKLKVTVKIFGRSTPVELNYMQVEKIN"
     gene            35186..35629
                     /gene="rplK"
                     /locus_tag="PLO70_01890"
     CDS             35186..35629
                     /gene="rplK"
                     /locus_tag="PLO70_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQZ73229.1"
                     /translation="MAKEISGFVKLQCKGGQANPAPPIGPALGSKGINIMEFCKQFNA
                     RTQDKMGKVIPVLITVYSDKSFDFVLKTPPAAIQLMEAAKIQKGSKESNRAKVGKVNW
                     EQVKAIAEDKMPDLNCFTVESAMKMVAGTARSMGLNVEGKAPWEN"
     gene            35655..36350
                     /gene="rplA"
                     /locus_tag="PLO70_01895"
     CDS             35655..36350
                     /gene="rplA"
                     /locus_tag="PLO70_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQZ73230.1"
                     /translation="MAFISKKRKVADTKVDANKAYSLKEASTLVKSITTCKFDASVDL
                     HIRLGVDPKKADQAIRGTVTLPHGTGKTKKVLVLCTPDKEAEAKAAGADYVGLDEFVT
                     KIEGGWVDVDVIIATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVANAINDVKGGK
                     IAFKVDKVGIIHASIGRVSFAPEKIAENSQELLNAIIKAKPSSSKGTYLRGISMASTM
                     SPGIAVDTKAFVH"
     gene            36379..36903
                     /gene="rplJ"
                     /locus_tag="PLO70_01900"
     CDS             36379..36903
                     /gene="rplJ"
                     /locus_tag="PLO70_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQZ73231.1"
                     /translation="MTKDQKNEVIELLKGKFSQYNNFYVADTESLTVAQVGKLRRACF
                     SKQVEMKVAKNTLIKKALESLDSEKYAGMYDSLHKVTALMFSENPKEPALIISSFRKE
                     SNGEKPVLKAAFINGDVYMGDNQLKALTQIKTKNELIGEVIGLLQSPAKRVLAALLHH
                     HEQKAGSAEVAAAE"
     gene            37059..37433
                     /gene="rplL"
                     /locus_tag="PLO70_01905"
     CDS             37059..37433
                     /gene="rplL"
                     /locus_tag="PLO70_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQZ73232.1"
                     /translation="MADVKALAEGLVALTVKEVQELADFLKSEYGIEPAAAAVVVAAG
                     GGEGAAAAEEKTAFNVVLKSGGASKLNVVKIVKDLTGLGLKEAKDLVDGAPKNIKEGV
                     SKAEAEDIAAKLKEAGADVEIA"
     gene            complement(37481..38461)
                     /locus_tag="PLO70_01910"
     CDS             complement(37481..38461)
                     /locus_tag="PLO70_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQZ73233.1"
                     /translation="MNWKEFTDDYLNFSRKDRIGITVILGVILSAFFLPNAFSKGKSL
                     PKENSSDTSWITSMLKLEQKETEAQDEPGKFADDNSSHYQYDRSTSDYYGKTKVNLFY
                     FDPNTLSPEGWQRLGLRDKTIKTIQNYLSKGGRFKNQEDLQKIYGLFPDEYKRIAPYI
                     RIETDLQSKNTQDYAHNEAPKESYPSKKYTPPYYTIIDINETDTTALIALPGIGSKLA
                     ARIVNFRDKLGGFYSVSQVGETFGLPDSTFQKIKQYLKVENTTIRKLNINTATLDELK
                     AHPYIKWGIANPIVAYRKEHGPFSRVEDIKKVMAVTDELYNKISPYLVIQ"
     gene            38728..42531
                     /gene="rpoB"
                     /locus_tag="PLO70_01915"
     CDS             38728..42531
                     /gene="rpoB"
                     /locus_tag="PLO70_01915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQZ73234.1"
                     /translation="MSSTKMNSRVDFGKIKHLAETPDLLEVQIQSFKEFFQLETTPDK
                     RNIEGLFRVFKDNFPITDTRNIFVLEFLDYFIDPPRYTIEECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGNIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSVH
                     PNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRSIGYETDKDILE
                     LFGMADEVKTDKKTLSKYVGKRLAARVLRTWVEDFVDEDTGEVVSIERNEIVMERDTV
                     LDEEAIETISEMEVTSVFIQREDVGGDYAIIYNTLNKDTSNSELEAVQFIYRQLRGAD
                     APDNETARGIIDKLFFSDKRYDLGEVGRYKINRKLNVDLPLEKKTLTREDIILIIKYL
                     VRLTNGKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTPV
                     DLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEVR
                     DVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINDMGFIETPYRKVHEGKVNMKELSF
                     LSAEEEDLAKIAQANTPLDEKGNFKEEKIVSRETGDFPILDKNDVEFMDVAPNQIVGL
                     SASLIPFLEHDDANRALMGSNMQRQAVPLLRPDVPIVGTGLEGKAARDARIQIHADGN
                     GVVEFVDANEIHVRYDRDEAEKLVSFEDDLRVYRLTKFIKTNQETCINLRPAVKKGQK
                     VKKGDFLTEGYATQNGELALGKNLKVAFMPWKGYNFEDAIVISERVVKEDLFTSVHIS
                     EYELEVRDTKLGEEELTPDIPNVSEEATKDLDENGIIRIGAQVKEGDILIGKITPKGE
                     SDPTPEEKLLRAIFGDKAGDAKDASLKAPNGVEGVVIGKKLFQRAKKDKNAKVREKAA
                     IEKLEKMHEKNETDLMEVLLEKLGMLLKDHVSAGVGNNFGEIQIGKGAKFTEKNLRGL
                     DYANINPLGWTGDKKIDDQINTLLHNYNIKFNEELGRYKREKFNISIGDELPAGVLKL
                     AKVYLAVKRKLKVGDKMAGRHGNKGIVAKIVRHEDMPFLEDGTPVDIVLNPLGVPSRM
                     NLGQIYETILGWTGEKLGLKFSTPIFDGATVEEIAEYCKQADIPMYGHTYLYDGETGE
                     RFHQKATVGIIYVIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAYGASNILQELLTLKSDDIIGRAKTYEAIVKGENVPRAGVPESFNVLVHELRGLGL
                     DLKFE"
     gene            42625..46917
                     /gene="rpoC"
                     /locus_tag="PLO70_01920"
     CDS             42625..46917
                     /gene="rpoC"
                     /locus_tag="PLO70_01920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQZ73235.1"
                     /translation="MAIRKENRPKAAFSKITIGLASPDTILERSYGEILKPETINYRT
                     YKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGVSSKKLETIVYYERFVVIQSGIRADKGQNPGDLLT
                     EEEYLDILDTLPKDNQYLPDDDPNKFIAKMGAEAVHDMLARIDLDQLSFDLRNAAATE
                     TSQQRKADALKRLSVVEAFRDANTRISNRPEWMVMQYIPVIPPELRPLVPLDGGRFAS
                     SDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAEG
                     GRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSARKLVDKKEAVVWDILENILKGHPVMLNRAPTLHRLSIQA
                     FQPKLIEGKAIQLHPLVTTAFNADFDGDQMAVHVPLSHAAVLEAQLLMLASHNILNPQ
                     NGTPITLPSQDMVLGLYYITKGKKTTATEIVRGEGKVFYSNEEVIIAYNEGVVDLHAW
                     IKVKANIRNADGLLEHKLIETTVGRVLFNQHVPAEVGFVNALLTKKNLREIIGDIIKI
                     TNVPKTAKFLDDIKTLGFRTAFQGGLSFSINDLIIPDTKQELLESAKGEVDEVWDSYN
                     MGLITNNERYNQIVDIWSRVDTRITETLIRELANDRQGFNSVYMMLDSGARGSKQQIK
                     QLAGIRGLMAKPRKSGSTGSEIIENPILSNFKGGLNVLDYFISTHGARKGLADTALKT
                     ADAGYLTRRLVDVSQDVVITEEDCGTLRGIATSALKDNEDIIEPLSDRIAGRTSLHDV
                     YDPISDQLIVSATEEITIDIAKKIEEAGIETVEIRSVLTCESKRGVCVKCYGKNLASG
                     NIAQKGDAVGIIAAQSIGEPGTQLTLRTFHVGGVAGSASVESTLAAKFDGTIQFDGLR
                     TVVIENNEGEKTQVVIGRTGEVRIIDTKNDRLLITNNIPYGATLNVKDGQKVNKGDVV
                     CTWDPFNNVIMAEINGTVKFENVIDGVTYREEADEQTGHREKVVIETRDKTKIPSILV
                     EGKDKKSYNLPTGSHIIMEEGDDVKAGQVIVKIPRILGKLRDITGGLPRVTELFEARN
                     PSNPAVVSEIDGVVMMGAIKRGNREIIIEAKDGVQKKYLVPLTRQILAQDGDFVKAGA
                     SLSDGQIAPFDILSIKGPFAVQEYVVNEIQEVYRLQGVKINDKHIEVIVRQMMRKVNI
                     VDPGDTRFLEDDLTDKFEFVEENDYIYDKKVVTEVGDSTKLRAGQIVSLREVREENSI
                     LRRNDKKLVEYRDAKPATSSPTLLGITKASLGVPSWISAASFQETTKVLSSAAINGKT
                     DDMLGLKENVITGHPIPAGTGLREFENMIVGSKEEYELLQTTREAMAFDEEE"
     gene            47064..47678
                     /locus_tag="PLO70_01925"
     CDS             47064..47678
                     /locus_tag="PLO70_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQZ73236.1"
                     /translation="MNLMKNGLFIWNVVLTLASGYLLVSHFSGKKITGTVGKDNGKAT
                     SSSSNSFRIAYFEMDSLAANFDMVKDLKAEMIKREEAINAEMDRLSKNLQQKFNSYQQ
                     QATSGSMTEEQSEAASRDMKNLDDQMKSRKQALDSDYSDFVMRRQNEIKSKIETFLKE
                     YNRTKDYSYIVSYEQGLFYYKDTAYNITADVVKGLNELYKTKKN"
     gene            47766..48755
                     /locus_tag="PLO70_01930"
     CDS             47766..48755
                     /locus_tag="PLO70_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HQZ73237.1"
                     /translation="MEDFKKLFTSFPAVKVGVIGDVMLDTYMWGKVERISPEAPVPVV
                     TLQKKEYRIGGAGNVALNCRSLDAQVSILSVTGDDSDSVILKQLLEDNKIDTSYLVPS
                     ARRITTNKTRIISRNQQMMRLDAEVTNDLDVIDEKLLLEKVEAYINTEQPTVIIFEDY
                     NKGVLSEYTIKQIIQWCKQSGIITAVDPKRKNFFSYTGVDIFKPNLKEVKEGLNLLVD
                     NINESVLKNIHTELCRVLNHRISFITLSEKGVFYQQDEIGHIISSHIRNIADVSGAGD
                     TVIAVASLVYAATKNVQLMAEVANIAGGLVCEEVGTVAINKEKLLKECELLLC"
     gene            complement(48741..49415)
                     /locus_tag="PLO70_01935"
     CDS             complement(48741..49415)
                     /locus_tag="PLO70_01935"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HQZ73238.1"
                     /translation="MFKRNISGSDLLLIIANLIPVFGVWFLGWSPTEAFIVYAMETMI
                     VGILTILKMLIATIAKGKDEWHANGKTTLKSGFFFIFFFIIHFGIFAMVQTTIFSQAA
                     QITPPNSGMMHFFFKWYTYINEDIAWMLGAFIISYIAKSFIPFLLNGDYKTMPIMAIM
                     FQPYGRIFIQQFTVILGSMFLSLGLGKIFILVFALAKIAFELLIDYDKLMGKAMNDLK
                     KSSAKK"
     gene            49481..50413
                     /locus_tag="PLO70_01940"
     CDS             49481..50413
                     /locus_tag="PLO70_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQZ73239.1"
                     /translation="MLKFTIVIHGGAGTILRSAMTPELEKAYKEGLEQALHAGYTLLE
                     KNGTSLDAVKAAVMSLEDCILFNAGRGSVFAKDGSQEMDASIMEGKTLMAGAVSAVRS
                     IKNPVDLAYVVMTKSQHVMLNGKGAYDFALRHGIKTEPDSYFFSQFRYDQWKELQGTD
                     EAALDHNVPSKEKKFGTVGAVALDKHGNIAAATSTGGMTNKQYGRVGDSPIIGAGTYA
                     NNKTCAISCTGHGEPFIRSVTAYDVSCLMEYKGLSLNDAMHLVVHDKLLKIDGEGGMI
                     GVDAKGNAAMVFNSEGMYRGMKCSDGRNEIAVYK"
     gene            50444..51136
                     /locus_tag="PLO70_01945"
     CDS             50444..51136
                     /locus_tag="PLO70_01945"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQZ73240.1"
                     /translation="MQKYAIIVAGGSGTRMGSSLPKQFILLKDKPVLYYTLKAFLEAY
                     DDLQIILVLPVEYTNMGQEIIDAYFDKERIRITAGGDTRFQSVKNGLAMVEQESVIFV
                     HDGVRCLLSKELIQQCYQQALETGTAIPAIVSKDSIRLVNEKGNEAFDRNKVMLIQTP
                     QTFHSKILLPAFQIDYKDKFTDEATVVEAYGMKVSLIDGEENNIKITRPIDLLFAEKI
                     IEERGRQQEADL"
     gene            complement(51139..52059)
                     /locus_tag="PLO70_01950"
     CDS             complement(51139..52059)
                     /locus_tag="PLO70_01950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ73241.1"
                     /translation="MSAPLKIQYLTHYQIDKAKWDDCIGKADNGLIYAYSFYLDQMSK
                     NWDALVLNDYAAVMPLTWNRKYGINYLYQPFLTAQLGVFGNKITEEQVGTFIQSIPSS
                     FRFIDIALNSGNISGVPTGFSIHRSNYVLDLNNSYAELYKGYRENIKRNIKKANQLGC
                     RVERDFDAGKVIELALQQMEGYGKESAYNVDRFRKLYTYLHEKKMATTYGISLNEELL
                     ASCVFFFSHKRAYYILVGNHPNGKTIGASHALIDAFIKDHAGEYMSLDFEGSDIRNLA
                     FFYASFGAKQEVYPALKINRLPFYLKWLKN"
     gene            complement(52059..54884)
                     /locus_tag="PLO70_01955"
     CDS             complement(52059..54884)
                     /locus_tag="PLO70_01955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ73242.1"
                     /translation="MRKLLIFLFTLTSSRQVALSQYWQQQVNYSIDVSLDDKGHTLNG
                     FEKIEYINNSPDTIKYIWFHLWPNAYKNDKTAFSDHLLENGSTRFYFSDKEDKGYINK
                     LDFKVNNITAQVEDHPQHIDIVKVLLPAPLSPGQKTIITTPFHVKLPYNFSRGGHDGQ
                     SYQATQWYPKPAVYDKNGWHPMPYLDQGEFYSEFGNFEVNITVPKNYVVAATGELQHA
                     AETEWLKSRAAFTWEPVKQKTKTTGGQVKTSYQLFPETEKEIKILTYKQNNIHDFAWF
                     ADKRFIVNYDTCKLASGKVIDVFTYYTTPQKEVWKSSLLYAKDAIQHYSSLVGEYPYN
                     VVSVVQGPASFGGGMEYPTITVLSPEKSSRSLNFTIAHEIGHNWFYGILGTNERKYAW
                     MDEGMNSFYESKYQQRQNDHFGQRTEQSLLETFIVEKKDQPIGTSSEDFNEPNYGLIA
                     YYKASKWMTLLEKQLGTAVFNKAMQEYYRQWQFKHPQPEDFKKILEDISGKNLDTTFA
                     LLRQRGNLPGSEKKGIGFQGPIGAVSGVNSNKKSAITLLPILGSNSYNKLMAGALLTN
                     LRLPPSRFQFLIVPMYGFASKNFNGIGFINYSFYPNKVFRKIDIGISGSTFTVDRFTD
                     IDNNKTFLGFSKIVPGIRLTLNKKDPRSSLNRYVQFKSFLINEDGLRFYRDTVISGPD
                     TSILNKYKTTSENRTVNQVQFVIENNRALYPYRGELKIEQGKDFIRTAFAGKYFFNYA
                     KEGGLNVRLFAGKFFYSTSKTITKQFATDRYHLNMTGANGYEDYTYSDYFIGRNKFEG
                     PPSQQIMERDGAFKVRTDLLAEKVGKTDDWLIAANFSTTIPSSINPLSLLPVKIPLNL
                     FFDIGTYAETWKQGAETDRFVFDAGIHIPLLKETVNIYVPLIYSNIYKDYIQSTIEKK
                     GRFWKKISFSIDISNFSFRKFDRNLDF"
     gene            complement(54966..56381)
                     /locus_tag="PLO70_01960"
     CDS             complement(54966..56381)
                     /locus_tag="PLO70_01960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQZ73243.1"
                     /translation="MQSGKQISLAWYVAVDFVMAALTWLCFYFWRSWMLNDAGAYPIS
                     YTSWLYILLIVPAGWLVLYTIAGTYNYLYKKSRLAEFTLTFVCSLIGVIVLFIVFVLN
                     DFDPNNGYAYFYLAFATLLSIHFLLTFSGRWIILNAIKRQLLRGKVFFNTLMVGSQEN
                     AIRIFKETENNLHDGGYRYAGFITPDNNGRNGIHKLLPKLGTVHELEKIIDQTNIRLV
                     VLALEKTEQPLLENIINRLSEKDVEVKIQPDTLDILSGSVKTSNVMGAALIDLQTGLM
                     PQWQQNFKRLIDVTSALFGLVLLSPFMVYIALRVKFSSKGPVFYSQERIGYKGKPFTM
                     YKFRSMINEAEKDGPALSSENDSRITKWGKVMRKWRLDELPQLWNILLGEMSLVGPRP
                     ERRFYIDQIIARNPYYKYLLKVKPGLTSWGMIQFGYAENTDEMIERSKFDLLYIENIS
                     LVLDFKIMIHTIRIILKGKGK"
     gene            complement(56381..57355)
                     /locus_tag="PLO70_01965"
     CDS             complement(56381..57355)
                     /locus_tag="PLO70_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73244.1"
                     /translation="MLKIGVFGVGHLGKFHLNNWKEIAGAELVGFYDPHDETAKEVSE
                     KYQLPRFLDADALIDACDAIDVVAPTNYHFEWCEKAIKKGKHVFVEKPLANTMEEARQ
                     LVKLAAESGIKFQVGHVERFNPAFLAIKDMELNPMFIEVHRLAQFNPRGTEVSVILDL
                     MIHDIDIILSIVKSDVKSISASGVGVLTETPDIANVRIEFHNGCVANLTSSRISMKKM
                     RKIRLFQKDSYIGIDFLNKKTEIIKLKGPGDTNVFAFDIETPTGKKTIAMANPVVPEV
                     NAIKMELEEFKNAILNNTKTIVSEMDGLMAMDVAHQILEKIGNNNISH"
     gene            complement(57425..58177)
                     /locus_tag="PLO70_01970"
     CDS             complement(57425..58177)
                     /locus_tag="PLO70_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQZ73245.1"
                     /translation="MKDRFKGFYLYLSSLVIALLHLPFAFAKSASGSSRMSFHPGDSA
                     IRNTSDSMYFAPETRSLYDSLHLSLLGLSKDAFDYAKRGFDKLIEEGKLLNNSIISIV
                     DFSQPSNKKRLFVIDLKNYKVLFNTLVAHGRNTGRELATSFSNQSSSYKSSPGFYVTR
                     ETYQGKHGLSLKLEGIESGINDKAYQRGIVVHGAEYVSDAIANAQGFIGRSQGCPAVP
                     VHTSTPIINTIKNGTCYFIYHPSYIDQSEVLN"
     gene            complement(58345..58418)
                     /locus_tag="PLO70_01975"
     tRNA            complement(58345..58418)
                     /locus_tag="PLO70_01975"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58382..58384),aa:Asp,seq:gtc)
     gene            58613..59584
                     /locus_tag="PLO70_01980"
     CDS             58613..59584
                     /locus_tag="PLO70_01980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQZ73246.1"
                     /translation="MPSDRNRQFIDFEKPIKDLLEEIEDLKQKAEKSKVDYSEVIHKL
                     ENSVLEKRKEITQSLTSWQRVQLSRHPDRPYTLKYITKMTSNFVELYGDRNVKDDKAM
                     VGGFASLDGETVMIIGQQKGINTKMRQLRNFGMANPEGYRKALRLMRLAEKFNKPVIT
                     LIDTPGAYPGLEAEERGQGEAIARNIYEMIRLKVPVICVIIGEGASGGALGIGAGDKV
                     FMMENTWYTVISPESCSSILWRSWDKKEVAAEQLRLTADKMLGFGLIDGIIPEPPGGA
                     HWDYDEAAQILKNYLVPVLKELKQIPVEQRVNNRIEKFGKMGFWEEV"
     gene            59749..60636
                     /gene="dapA"
                     /locus_tag="PLO70_01985"
     CDS             59749..60636
                     /gene="dapA"
                     /locus_tag="PLO70_01985"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQZ73247.1"
                     /translation="MSLRKKFTGTGIAIVTPFHADGTIDWDSFKKLIQFWIEGKTEYL
                     VVLGTTGESATVHGEEKQQVFSFVKNEVAGRLPLVAGIGGNDTHEVIKGLKEFDLSGY
                     DAILSVSPYYNKPNQEGIFQHYKALDAATPLPIIMYNVPGRTGQSVTADTQIRIAREC
                     KNVFATKEASGNFDLINQIIRNKPDDFMVISGDDPITLHMIAAGAEGLISVVANAYPR
                     EYSDMVRFCLAGKFAEAQKLHYKYIDIITSMFAEGSPSGVKAYLSEMGLCQNTFRLPV
                     WKVSEKHHTKIKELMKDVG"
     gene            60650..61477
                     /locus_tag="PLO70_01990"
     CDS             60650..61477
                     /locus_tag="PLO70_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ73248.1"
                     /translation="MLLRICILNLLLLTGFYSFAQNETKPAIKSSASGNVIIIDTAFG
                     IPQLQRSRKIWIYLPAGYSTSKKKYPVLYMHDGQNVFDSATAYAGEWGVDEFLDSVKL
                     KESIVVAIDNGLNKRMNEYNPYDSERFGKGEGGFYVDFLVKTLKPYIEKKYRTKKCRK
                     SRFIAGSSMGGLISLYAVLKYPRVFGGAGIFSPAFWVAPKIYDDIQSRGKKVKGKLYF
                     YAGKQEGETMVPYMLKAFEALTSVSRSKISTVIRDEGKHNEATWRKEFPLFYEWLLK"
     gene            complement(61516..>61950)
                     /locus_tag="PLO70_01995"
     CDS             complement(61516..>61950)
                     /locus_tag="PLO70_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HQZ73249.1"
                     /translation="LNPDKAKEYEIPASFYERLTNGEDYTRKDGTLIKNETVTTPGDP
                     GRKYAFCADTKYDESLIPHVQGFDMIYHETTYLDNLRERAELRFHSTTKQAALIAKKA
                     GVRKLLIGHFSSKYDTLEEFEAEAREVFPNTDLALEGVAYTV"
BASE COUNT        19076 a        11237 c        13220 g        18417 t
ORIGIN      
        1 tattcagtat agtattgtct gcaaaaaata aacggcaatt attagtccca aaagggtttg
       61 ctcatggttt ttctgtgttg agtaagaacg ctgaagtatt gtataaatgt gacggctttt
      121 acaataaaga aagtgaagga ggaattgtgt acaatgatcc ggcactgagt attgactgga
      181 ggatacctgc agcagacgca atcatttcgg ataaagataa ggtactgcct gtgttggcag
      241 catgtaataa cagctttgta tttaatggct gatggaatca aaacaaaaaa tactcatcac
      301 aggttctaat ggtcagttgg gaaaagagtt aaagcggctg gaaaactctt ttccgcaatt
      361 caattttatt ttcctgtcaa gagaagatct gccgattcat cattatgaat tggtacgtaa
      421 tttttttaaa ggctatcagc cgcaattttt aattaattgt gcagcatata ctgctgttga
      481 cagggcagaa tcagaaaaag agctggcatt ccaggtaaat ggggaatccg taggggtact
      541 ggctgcagtc tgtaaagaat atcaaactaa gttcatacat atttctactg attatgtttt
      601 tgatggcaca gccaccgtcc cttacaaaga agattcactg accaatcccc aatcagttta
      661 cggggcatca aaattggaag gagagaaaga ggcactgcga ttcaatcctg attccattat
      721 tatccggaca tcatgggtgt attcagagtt tgggaagaat tttgtaaaaa ctatgctgaa
      781 attgatgaat gaaagagaag aattgaatgt cgttagcgat cagtttggtt cacccactta
      841 tgcggccgac ctggcggaag cgattttaca gatcatttcg tcggggcaat ggcatccggg
      901 tatttatcat tacagcaatg agggaaaaat cagctggtat gaatttgcgg tcgcaataaa
      961 agcattgtcg ggtaacgttt gtaaagtaaa cccgatccca acttctcaat atcccactcc
     1021 tgctaaaaga ccggcttatt cggtactgga taagacaaag attcagtctg cattcagtgt
     1081 acggattaag gattggaaga caagcctgga aacctgttta tcccggttaa aatgaaaaac
     1141 cccgccgttt agcggggttt cgttgaccca tatggattcg aaccataaat gactgaacca
     1201 aaatcagtag tgttaccatt acaccatggg tcaatcctgt tccaattttt caattgggag
     1261 tgcaaaaata gggctatatg ccttttctcc caaatggttt ttgaaagaaa ttatgcagtt
     1321 tgagcagttt tttgaccctc tttctcctcc cttaccttca attcttttat cttatccaat
     1381 gccctggcca ctacatcgca catgatcgtg tacagtgcac ccggttttgc attttcataa
     1441 atatagtcca gtgccttgtc ctcattagga attgtcaaca caggaatgtc aggattgacc
     1501 ttattgatac cttcttttaa caggtcaata atttcatccg ctgttcttcc ccgcaggttt
     1561 ttgtcgcaac ggataatgat ttcatcaaaa tgacttgctg atatttcacc taattcccgg
     1621 atatcttcat ccctccggtc gccagtgccg ctgatgacac cgattttagt gggataatcc
     1681 agtttactca caaaatcaca aagcagtctc agtccatgcg gattgtgggc aaaatcagcc
     1741 agcattgtaa aatgtttgaa atggaagaaa ttcaaacggc ccggtgtcag gctttcgctg
     1801 ggaatgaatg tttgcaatcc aatacgaata tcatctatgg taatgtcgcg gtataaatag
     1861 gcagccagca ctgcaggtaa acagttattg atattatgga cagcttttgc ttcataggtc
     1921 agtggaatgt cccttgcagc cagcaccctt atcttccagt tacctttcat gatagttaca
     1981 tacccatttt catatacaca ggctaatcca tgattggcac aatgttcctt tattcttgga
     2041 ttattctcat ccatactgaa cagggcgata ttacatttta aatccttctt cattttgtac
     2101 accaggtcat catccgcatt cagcacagca tatccatgcg gaaaaacggt ttccggtact
     2161 accgctttta cttttgccat ctgctccacc gactctattc caccaaggcc aatatggtca
     2221 gcagccacat tggtgaccac ggccacgtcg caatgctgga atgccaggcc agcttttaaa
     2281 ataccgcccc gggcacattc taataccgca aaatcaactg tcggatcttt taatacaaat
     2341 gttgatgaaa ccggccctgt gcaatcacct ttcatcatca gctgattttg gatatagaca
     2401 ccatcggaag tggtatatcc aactttcttt cctgcactct tggcaatatg tgcggtaagt
     2461 cttgtggtag ttgtttttcc attagtgccg gtaatagcaa taataggaat tctgcctgca
     2521 cttccttttg ggaataacat atccactacc ggttctgcca catttctggg cagcccttct
     2581 gccggttcaa tatgcatacg gaatcccggt gctgcattta cttctagtat tgctccgcca
     2641 ttttcagcaa caggagtttt caaatccata gccatcacat caataccgca aatgtcaagc
     2701 ccgatgatcc tggcaattcg ttcaaacatg aaaatattag ccgggtgtac ttcgtcggtt
     2761 atatcagtgg atgttccgcc agttgataaa ttagcagttg gtttcaataa aactctttca
     2821 cccttttggg gaacagtttc cattgtaatc cctgtatcat ccagcatttt ttgagtaaac
     2881 tggtcaacgg ttatttgtgt cagcactttt tcatggccat acccacgacg tgggtcttta
     2941 ttcacttcct caatcaaata ccggatatca tgaacaccat cgcctactac agatgccggc
     3001 gttctcaatg ctgcgcaaat gaatttataa ttaatcacca ggcaccggaa atcataaccg
     3061 gtgataaaac gttccacaat aacactccgg ctgtatgctt tggctgcatc aaatgcttta
     3121 atagcctgag tccggtttgt aatattggtg gtgttacctt ttccatgatt cccatcaata
     3181 ggcttgatca ccaatgggta accaaatttt tctattgcct ggtcaagtcc ttcctcagtt
     3241 ctgacaactg ttcctcttgg taccggaatt tcagcagctt ccagcagcat tttagtttct
     3301 tccttatcac aggcaatatc aacggcaata ttggaagtag ttgatgcaat cgtggccctg
     3361 attctttttt ggtttacccc gtagcctaac tgaactaaac ttgctttatt caaccgtata
     3421 taaggaatcc ctcttttagc agcttcatct acaatacaac cggtggaagg gccaagtctt
     3481 gtatcttctc ttatcacccg catttgctga atatcttcag tcaaatcata atccactcca
     3541 ctgatcaatg cctgggcaat gcgaactgca gctttagcag catatacacc tgcatcttct
     3601 tccatatagc taaatacaac ataataaata ccttcttttt ctccttccgg agttctggtt
     3661 cttccaaagc cggtatccat tcctgccagt gtctgaattt ccaaagcaat atgctctact
     3721 acatgtccca tccaggtgcc ttcatccacc cgttcaaaaa aaccacctgg tttaccaacg
     3781 ctgcagcggt gttcgtataa agaaggcagc aatttttcca gcctctccct gaagcccgga
     3841 atcttatttg taggcatatt ctctttttcc tccaggtcca gtttcatttg tatgagtttg
     3901 gtccggcgga cactccagta gtttgggcct ttaagcactt taatctccag tatctgcatg
     3961 tgttttttaa tttaagatcc cgttataacc gggacaggtt ggaagatagt tttttttgct
     4021 tatgtagcca acagttgtac tgctatcatc gttccttgcg tcgcacatag cagctactgt
     4081 atttctgtgg ggcagtttcg gatgtaactg tgtatttgcg ggaacctttt cagtacattt
     4141 aggaacttaa attgattatt atgtcagcaa ccaaccgtcg aggctttctg caaaaagctg
     4201 gtatgttttc tgccactgca tttctttctt cattgggaca acctgtatgg agcaggaatt
     4261 tggaaaatgt cctgcgcaat gcagaaggaa tttccccgga agatttagca gcagaagaag
     4321 atttttggta ttatatacag caagcattta ccgtttcgcc ttcattaatt aatttgaata
     4381 acggcggtgt ttcccctgct cctaaaacag tgcaggaggc gatgaagaga tattatgacc
     4441 tctgtaatga agcacccagc tattacatgt ggcggatact cgaccagggg agggagccat
     4501 tgagaaaaaa cctggcaaag ctgggcggat gttccccgga agaaatcgct atcaacagga
     4561 attcatccga aggattggag acagtcattt tcggattaca attaagagaa ggggacgaag
     4621 tagtagctgc taaacaggat tatcccaata tggtgaatgc ttacaagcaa agggaaattc
     4681 gggatgggat taaaataaaa tggataagtc ttgaactacc aagcgaagat gaaaattatt
     4741 tggtgcagca gtatgtaaac gcatttacag ataaaacaaa agtggttcat attacccatg
     4801 tcataaactg gaatggccag atattacccg taaaaaaaat tgcacaggaa gcccataaac
     4861 gaggaattga agtggtggta gatggtgcac acagtttcgc ccatttcgat tttaagatcc
     4921 ctgacctgga tgcagattat tttgctgcca gtttgcacaa gtggttatat gcaccaattg
     4981 gcagtggtct tttatatgtc aggaaagaaa agataaaaga tatttatccc atgtttgccg
     5041 ttgataaacc gctaaaagat gatatcagga aatttgaagc cttgggtacc cgtccttttt
     5101 atattgaaca ggccattggt aaggccatag aatttcatga aatgatcggc atcgaacgga
     5161 aagaaaagcg gttgcacttt ctgaagaact actggatgga aaaagtaaaa aatataccaa
     5221 aagtaaaact gaatacatcc ctccatccga aatggggctg cgcaattggt aatgtgggta
     5281 tagaaggaaa gaaacccaat gaactggatt cattcctgct ggataaatac aaagttcaca
     5341 ctgtagccat tacctgggaa aatattgtag gggtgagggt aacgccgaat gtttatacaa
     5401 ccactaagaa tttagatgta ttgatagagg gaattagtgc gtttgcaaag agttaaataa
     5461 gccacgaacc ataaaccatt tactttcttg tccataccgc ttactaaccg aaaaataaat
     5521 ctgcttcagg ccacctctat caatatgata gatatggtag gcatcggtcc atttgtggta
     5581 atgcctttcg tagtggcaca gttcaataat ggattgttta tttgggcatg gatatttggg
     5641 gcattcacag cttttatgga tgctatgata tggagtgaac tgggtgcaaa atatccttta
     5701 gccgggggta cttacaattt tcaccgtata gcatttggag aaagaggagg gaagctgatg
     5761 agttttttat ttgtctggca aacatctata caagcaccac tggtggttgc atctgcttcc
     5821 ataggatttg cgcaatacct gacttatatt gttccgttgg atatttggca acagaaaatt
     5881 gtttctggtg gattggtgat gctggtcttt atattgcttt accgcaagat tgaaactatt
     5941 ggaaagattt ccgtggtaat gggaactatc gttgtgctca caatcatatg gatcattatc
     6001 agtggattat tgaatcaaca acacccggtt aaactcatcc cgacaggtaa ggagtcattt
     6061 tttacctatg ctttctgggc agcagtggga caagcttcgg taaaaacagt gtatgcttat
     6121 ctggggtatt ataatgtttg tcatctcggt ggagagataa agaatccggg taaaaatatt
     6181 ccgcgaagta tttttatttc agtttttggt attgccactt tgtatctatt aatgaatata
     6241 agtgtgatgg gggtgatgcc atgggaattg gttaaggcag atgataaata cctggtgagt
     6301 tcttttatgc agcagttgta tggaactaag gcaggtgttc ttgttacaat attgattctt
     6361 tgtattgcat tctcctccct ttttgcagtt gtgctgggat actcaagggt gccgtatgcc
     6421 gcggcagtag atggaaattt cttcaaacct ttcgccaaac tgcacccgac aaagaatttc
     6481 ccctacatct cgctaattgt gctgtgtaca ctgggtttta tattcagcct gttgttcaaa
     6541 cttgcggaag tgatcagttc cattttagct atgcggattg tcatacagtt tattggtcag
     6601 gcagtagggg tcgtaatgtt gcggaagcga tttggggaaa aagatcttcc ttttaaaatg
     6661 atgctgttcc ctttaccagt tatcctctcc attgttattt gggcattcct gtttgtttct
     6721 accggttggt ttgcattgtg gggaagtttg attgcattat gtggcgttgg ggtttatttt
     6781 tttaccagca ggcttaagaa gtcggagtag ttaatatttt ccacatatcc acaagccagc
     6841 ctttcctgtt gacaggtata ttatttttct attacatttg ttgataatca aaccccaatg
     6901 aatgagtaat ccgaagggaa aactgatagc cataggtgga gctgaacaca aaggcactga
     6961 attggaagca gacggctttc atcgaaataa tcttaatttt tttgaactcg gcattcttcg
     7021 ccgggtagta gaagaagctg gtggtctcaa tgccaggatt gaagtaatta ccactgcttc
     7081 aatgattccc tatgaagtag gagaaaatta tctgaatgct tttggtaaaa tcgggtgtac
     7141 caatataggc cttttacata tccgtactag gcaggatgcc atgaacgagg aatatgtgga
     7201 tcggatccgt cactgtgatg cagttatgtt tagcgggggt aaccagcttc ggcttagtgc
     7261 tacagatggc ggaactgagt ttcttactat tctgaaaaaa agatacaagg aagaagagaa
     7321 ttttttaatt gcaggtacct cagccggagc aatggcaatg agtaatacaa tgatctatga
     7381 gggtaatgct accagggccc atttaaaggg cgaagtaaaa attactaccg gtttgggttt
     7441 tatgaacaat attattattg attctcattt tgaaaaaagg ggacgttttg gcagattggt
     7501 acaggcagta gctgctaacc cggggtgtat tggtgtgggt ttaggagaag ataccgggat
     7561 gcttattaca gaaggaaata aaatggaagc catcggtagt gggttggtgg ttatcatcga
     7621 cggtcatgat atacttcatt ccaatattgc ggatatacca gacgggaacc ctattagcat
     7681 tgaaaacctc aaagtccatt tctgcgaaaa agggaacggt tacctgataa aggaacggag
     7741 atacctgccc gaggccagtg acggctcagt aattaaaaaa caggtggatg tggaataaca
     7801 ctaattctct tagcagtaag ttgagagaat tccggttaca ggaacgcctt tttgtttgaa
     7861 aagaatctac cttcaccatc cattcttccg gccttttgta atacactatc cctgcccggc
     7921 cattttataa ctttaaacaa actctcatga aactttttca acaacgttta ctggcagttg
     7981 cggctttggc tatattagtt tttgcagcct gtaaatcaaa aacagtaaag gaagagaaac
     8041 cacctgttat tccggtagaa aatttcttta aaaatcccga gaactttggc tggcgtattt
     8101 ctcccgatgg tgagtatatt tcttacctgt cgccatacaa gggacatact aacgtatttg
     8161 taagaaagat tgcagacagt gtagccattc ctgttacttc tgattcggtc cggaatattt
     8221 ttgactatca atggaaaggt aacaggatat tatacctcca ggatattggt ggtgatgaga
     8281 actttcaact tttttcagtg ggattagatg gaaagaatct gaagccactt acaccttttg
     8341 caaaagtccg tacctccatt ttaaatgatc tcaggtatgt gccgggtaaa gaaaaagaag
     8401 tgatgattca gatgaataaa agagaccccc gcttttttga tccgtacagc atcaatattg
     8461 agacgggtga aatgaaagta ctttttcaga atgataagaa ttatgacggc tggtttacag
     8521 atcatacagg gttgataagg attgccacca aaacagatgg tgtaaatact acttactatc
     8581 accgtgccac agaagatgat ccttttgatt cactactgac aacaacatat aaagacaatt
     8641 ttggtatcca gctatttact tttgataata agaatcttta cgtggcttcc aatattggaa
     8701 gagataaaac taccatcctg gagtatgacc tagctgcccg gaaagaggta aaagaaattt
     8761 acagtaatcc tgattatgat gtggacggac tttcctggtc gccaaaaaga aaagtattgg
     8821 agtctgctga cttcacctca tggaaaagag aacaaaaatt tcttgataaa gaggcagaaa
     8881 ctgaatataa caagatgaaa gcgaaatttg aagggtatga agttgggcta tatggtagta
     8941 atgatgcaga agacaagttt gttgtgtgga ctggtaatga taaactggcc gctaaatatt
     9001 atttctatga taagaaaagt ggtgaaacca aatttctgtc cgatgctttt ccctggttga
     9061 aggaggaaaa tatggcttca gtaaaaccag ttgaatataa atctagggat ggccttacta
     9121 ttcatggtta ccttactttg cctaaaggaa tagaagcaaa aaatcttcct gtagtaatta
     9181 atccgcatgg aggtccctgg gccagggata gttggggata taacggagag acacaattcc
     9241 tggcaaaccg cggctatgct gttttgcaga tgaattttcg tggttctacc gggtatggga
     9301 aagaattttg gctgaaggga gctaaagaat ggggcaaaaa aatgcaggat gatattacag
     9361 atggggtaca atggttgata accgaaggta tagctgaccc gaaacgggtt gctatttatg
     9421 gaggtagcta tggcggttat gcaacattgg ccggtgtagc tttttcgcct gatctgtatt
     9481 gttgtgctgt tgattatgtt ggcgtttcca atatgttcac ctttatgaaa acaattccgc
     9541 catactggga gccctataag gccatgtttt atgagttggt aggtgatccc gtaaaagaca
     9601 gcgcattgct ggcagaagtt tctcctgctt ttcatgctga taagataaaa gctcctttgt
     9661 tcattgcgca gggggcaaat gatccccgtg tcaacaaagc ggaaagtgac cagatggtag
     9721 aagcactgaa aaaaagaggt attgaagtgg agtacatggt caaagatgat gaagggcatg
     9781 ggtttgccaa tgaaaaaaat tcaatcgatt tttataaagc aatggaaagc ttttttgccc
     9841 ggcatttgtc tgcttctaaa gaagaagtga aaaaataatg ggatacaact gtttcgtttg
     9901 taaaagcatc ctgatttttc agggtgcttt tgttttatat tgcggtacta atattttact
     9961 atgaaaaagt tattatcact tgttatcctg gttgtggtta gtatggcatt tactccccaa
    10021 aagaaaacca aagtcatctt ctttggggat tctattactg agctgggtgt aaaaaaggat
    10081 aagtatgttg gttacatagt acgaatggat agtatgctca aagccgtgaa aaaagatgat
    10141 cagtacgagc tgatagggtc cgggataagt gccaataagg tttacgattt gtatttacgg
    10201 ctggaggaag atgtgctagg caagaatccg gatattgtag ttatatatgt tggtgtgaat
    10261 gatgtatggc ataaaacact gcttgggact ggtaccgacc ccgataagtt tgagaaattt
    10321 tataatgcca ttcttagaaa attgaaggaa agaaatatta aagcaattct ttgcactcca
    10381 gcagtggttg gggagaaaac cgatatgagt aacccgttgg atggtgacct gaaccggtac
    10441 agtaatatca tcagggatat tgcaaagaag aatgatcttc cattagtgga tctgcggcaa
    10501 aaatttcttg attataacaa cactaataac cctgagaata aggaaaagga catccttact
    10561 tacgaccggg tgcatctgaa tgccaaagga aatcaattag tggctgatgc gatgtggaag
    10621 gccataaaag aaattaaata accttaacca ggtaataatt ctttttcccc ttttgcacaa
    10681 gtatatactt atcatgtaat agcagtgaag gatcaatttt aaaatcagcg gcttctactt
    10741 tctttctgtt gatacttaca cctccgcctt gtacattttt tcttgcattt cccttactgt
    10801 caaaaatggc tgtttctgcc aggaaagaaa caatgtctat gcctgccgta attttttctt
    10861 ttgaaaaatt gctttttacc acaccttcca gattttccag gtcttctgca ctgaggcttt
    10921 ctgccggagc attttgattt gcaaaaagtt tatgtgttgt ttcaattgct ttttcgtatt
    10981 cgtcttttcc atgtacaaaa ctggttattt cctgcgccag cgttttttgc aatactcttg
    11041 ctgccggatt catcttgtgt tcatgaacca gcatttcaat ggcatccttt gataagaagg
    11101 tgaatatttt aatccatttc tcagcatctg tatcactggc attcaaccaa aactggtaaa
    11161 attggaaagg ggaagttctg tttgcatcta gccatacatt tccactttca gttttgccaa
    11221 acttaccgcc atccgctttt gtaattaatg ggttggtaaa aacaaatgct tcacctccgc
    11281 attttcggcg gatcagttca gttcctgttg tcatattgcc ccactggtca ctgccaccca
    11341 tttgtagttt gcaattttta tgttgatata gccaataaaa atcataaccc tgcattaact
    11401 ggtaggcgaa ttctgtataa ctgataccgg tctcaccttc aatccttttt tttacactgt
    11461 ctttcgccat catataattg acagtaatat gtttaccggc atctctcagg aagtcaataa
    11521 atgaaatgga tttaaaccag tcataattat tcgccatctc agcagcattc tgcagggatg
    11581 gattaaaatc aaggaatttt tccagttgag ctttgacacc agccacgttt ttttgtaaag
    11641 cctcttcact cagcaaattt ctttcttcgc ttttaccgct tgggtcaccc accattccgg
    11701 tagcacctcc aacaagggca atgggtttat gcccggcttt ttgaaaatgt accagtaata
    11761 atatgggtac aaggctacca atgtgcaggc tgtcggcggt aggatcaaat cctatatagc
    11821 ctgtagtcat ttctttgttt aaatgttctt ccgtccctgg cataatatcc tgtatcatac
    11881 ctctccatct taattcttca ataaggttca taattctaag tcaatgattt acaatttata
    11941 ggcaatgaaa tcagccttta ccctgcaaaa gtaaggcagt ttggctttgt tggtctgacg
    12001 atacaatatt tttctaattt actcgttttc aatgcttagc cttttggttc aacaaaccta
    12061 tgattttaca ggcctaaaga ctaattttac gaacaatccg tatatactga agacaactct
    12121 tattccattg cttatttgaa aattcaatga atgaaaacga accgcttata cctaacccta
    12181 attgcgtttt ttctattcct taccagtaac gctcaatttc gtaaatactc taacgaattc
    12241 ctgaatatcg gtgccggtgc aagaggacta gcaatgggaa gtgcacaagt agcttctgtt
    12301 agcgatggaa cttctggtta ctggaaccct gccggtcttg tcagcataaa agatcatccg
    12361 cagatcaatt taatgcatgc agaatatttt gcgggaatcg gcaagtacga ttatgccagt
    12421 attgcttttc ctactgcaaa taataaaagg actatcggaa ttaccggact tcgttttgca
    12481 gtggacgata ttatgaacac attgttttta gtagaacccg atggctctat caattacaat
    12541 aatattcagt ctttttcctc agccgactat gctttcattt tctcttttgc gcaaaaaata
    12601 aaggaaacag aaagaaagaa agtaaacttt ggagtgaatg ccaaagtgat tcacagaagt
    12661 gttggaaaat ttgcgaaggc ctggggcttt ggtttggacg caggattgca aatccattcc
    12721 ggaaaatggc ggtttggtgt tgcaggtcgt gatatcacca gcacttttaa tgcatggtca
    12781 ttcaccttta cagaaagaga aaaagaagta ttgtacctga cgaataatga gattccggtc
    12841 aaatcaactg aattaacagc tccccggctt gtgctgggaa ttggccgtga attcaaacta
    12901 gggcagaaat caagtttgtt ggcagaagca aatatcgacc tgacttttga tggtcagcgt
    12961 aatacactta taagtgctga tccggtaagt gcggatccta aactcggctt ggaattgaat
    13021 gtgaacaatg tgttttttct gcggggtgga attaataact ttcaaaaagc cctttccgat
    13081 ggcgacacat taaaccagaa gaaagtgtgg atataccaac caagtgcagg agctggattc
    13141 cgtattcaga acgtagctgt tgattatgca ttttcaaatc ttgcaaacca gtcaaatcct
    13201 ttgtttacac atgttttttc tttgagactg aacctggtca atactagaaa caaaaactaa
    13261 ttaatctgtc tgtacctgat aaatctagat tcatgaaaaa atttttactc ccgttattgt
    13321 tatgcaccgg cctgctggcc caatcgcagg attataataa tgaatggatt aattataatc
    13381 tcacttatta taaatttaaa atcggagcta ccggacttta caggatcagt cagcctactc
    13441 ttgcaacgct gggtattgga aacacacctg ccgagcattt tcaattgtgg agaaacggcc
    13501 aacaaatacc tgtttataca actgttcaaa caggaccaat gggggtttct gattatatcg
    13561 aattttgggg tgaaatgaat gatggtaagc ctgataatcc actttacaga aacccggatt
    13621 ttcagctaaa tgataaatgg agtttggaga ctgatactgt ttctttcttt cttgctataa
    13681 atccaggtgg aaataattta cgtttggttc ccaccaataa tacgttacca tctgctttac
    13741 ctgctgaacc attttttatt catactgccg gtaaatacta cagggaaaaa ctaaatccgg
    13801 ggtatgctgc tgttgtcggc gaatatgttt attcatcttc ttatgaccag ggcgaagggt
    13861 ggacatccaa tgatcttgga actggcgtta caaggcctga aaatctttcg aatctttatc
    13921 cttatactgg cgctggtgct cctgctccat taattaaagt caatgctact ggtaatgcct
    13981 taaacccaag gcaatttgaa ataaaaataa atggaaccgt actgtctacg cagacaatgg
    14041 acttttttga ttataaaaaa gtacaggctg tattttctgc ttctttaata gggggtggtg
    14101 ctgccaatgt tgaggttaaa aatctttgta ctcttcctaa cgacagaatg tctatcgctc
    14161 aaacggaatt ggtatatgcc cgacagtttg attttggagg tgctaataat ttttcctttg
    14221 aactacctgc caatacagca ggcaactacc ttgaaattag tggatttaac catggaagca
    14281 taggcccggt tttctatgat ctgactaacg gtaaaagata cgttggtgat gtaacaactc
    14341 cttctttagt taaaatagcg ttggaaccgt cgtctgttaa caggaaattg ttgctggtaa
    14401 gccagcattc ctcatttccc gttaccgctg ttacattatt gcagcaacga aattttgtta
    14461 actatgctgc tgcttcaaac cagggaaact atcttattat ctctcatcct gctttgatga
    14521 acgggtccgg aggatcaaat cctgttgagg actataagaa ttaccgtagc tctgcagctg
    14581 ggggtagtca taacgcaaag atttacctgg tagatgaatt atttgatcaa tttgggtatg
    14641 gaataaagaa aaatcctctt gcattaagaa attttatacg ttggacccgg gctacttata
    14701 gctcaccagt taagaatgtg cttcttcttg ggaagggatt aaattatgtt cagtacaggg
    14761 catatgaaag taatccggat gtagaaagat tatctttcat tccaacattt ggttaccctg
    14821 catctgacaa tatgctggta gctgaacccg ggcttgatgg aatacccagg gttccggtag
    14881 gacgtatttc ggctataaat gcagatgaag tggcagttta ccttgcaaaa gttattcaat
    14941 atgaacaaca acaggctttt tcatctcccc tcattgccga taaagcgtgg atgaagaatg
    15001 tagcacatgt aattggggca gcagatggaa gtcttggtgc gattctgaca gctgatatga
    15061 ataggtataa atcaattatt tctgatacgt tgttcggggc ggatgtacac acatttagta
    15121 aggtttcttc tgcacctgtg gaacaggcga gcagtgaaaa gctgtatcaa ttatttcagg
    15181 aaggaatagg gttaatgact tattttggac actcttctgc cacaactctt gaatttaatc
    15241 tagataatcc cgatcaatat aataatccgg gtaaatatcc ggttacgatt gttatgggat
    15301 gtaatgctgg taatttcttt aattttaata cacttcgctt cataaccaaa gaaaccttat
    15361 ctgaaaaatt tgttttagcc aaccagcggg gctccattgc atttatagcc agtacccatt
    15421 tcggtattgt acactacctg gatattttca atacccgtac gatgacagct acttctgtat
    15481 ctcattacgg gaagacattg ggtgaaatac tgatagaatc aattacacag gtttacaatc
    15541 tgactacgca aaacgattat tatgccaggt ttcattgcga acaaaccaca ctgcacgggg
    15601 atccggcttt aaagtttgat gcatcaactc cgaaaccgga ttatgccatt gaagagcagt
    15661 tggtaaaagt taacccctct tttatttcag tagcagaaac acatttcaag gtggatgcga
    15721 agttcatgaa cctgggcaag gctataaaca aaaatattgt tgtagaagtt aaaagaacat
    15781 accctaacct gactacggaa gtaatccgca gggataccat acccggtatc cgtttcatcg
    15841 actctatttt atatgctatc gaaatcgtac ctacacggga taaggggttg aataaaatat
    15901 caatttgtat tgatgctgat aatgtggtgg atgagttgta tgaaacaaat aactgcatta
    15961 caaaagatgt gttcatttat gaggatgagg caaggcctgt ttacccgtac acatatgcca
    16021 ttataaataa gcaggatatt aaactggtag cttcaactgc caatcctttc agtgggataa
    16081 aacagtatac catggaaatg gatactactg aattcttcaa ttctccagcc aaaatcaccc
    16141 gtactataag ttctgctggc ggattattgg agtttactcc gggagttgcc tttacagaca
    16201 gcactgttta tcactggagg gtggcaccag ttcctaccag tggccagcct tcatggaata
    16261 agtcttcttt tgtgtatttg aatagtactg gtcctaatag tagtgacctt gggtataatc
    16321 agtctgactt ttatcaacat acgaaatcgg agtacgaaag actaaagatt gattctgcca
    16381 gccgctcatt aaagtttgga cctgttgtaa ataacctgtt tatcagactg ggtacatgga
    16441 taacaagtgg cgctacccag gaagcgtcat tggctatttc tatcaatggt gtttccgcaa
    16501 tacgattaat gtgttggttc tcatcattag tatttaacgt ttttgatcca gtatctttca
    16561 ggccatggca aaatcaaagg atagttgcga ataattatcc aacctcactg ggagaggggc
    16621 tttatggaag cactgataat aattgttttg ggagcaccaa gattttcaat tttgaataca
    16681 ggtacactga tactgctagt cgaagaaaaa tgatggattt tatgcgtgat gttataccag
    16741 atggggctta tgtggctgtc aggaatttta ctttagatcc ggcctttggt tttcccaatg
    16801 catgggcagc ggattgggca gcagatgagg ctattcatgg tcctggtcag tcattgtacc
    16861 actatttgaa gaatgccgga ctaagcagta ttgactcatt ttacagaacc aggccctggg
    16921 cattggtata taaaaagaat gacccatcat tcactcctaa gtggattatg ggtgatggta
    16981 tgtttgataa ccctacttta tcagttgatt gctcaacggc agatacagtt ggacatatta
    17041 cttcacccgt ttttggccca gccagagcat ggaagcaact taaatggaga ggaagcagtg
    17101 aaccggcagg ggataccgcc actgttgatg tgattggtgt cagggcagat ggaagtgaac
    17161 aaacactgtt caacggactt acaacagctc agcaggattt tgatgtttct tctattgatc
    17221 ccaatgtgta tccatatgtt aaattgaaaa tgaggacaga ggacaatatt aattttacac
    17281 cataccaatt acggtattgg agaatcacat atgtaccggt accggaaggg gcaattgctc
    17341 caaacttgca tcttgtagta aaagatacac tggaagttgg agaaccttat aattttaaag
    17401 taggattcaa aaatgtaagc gaggtggctt ttgacagtct caaagtgaaa atgataataa
    17461 ctgaccggaa taatgttcct catattattc cgattccaag acgcagacct ttgccaatta
    17521 atgatacatt acagataggc agtacagtaa acacagctgc aatccctggt gctaatacag
    17581 tatatattga agcgaatcct gataatgatc aattggaaca gtatcacttt aataactttg
    17641 cttaccgcag cctgtatatt aaacctgaca gtttgaaccc attactggat gttacatttg
    17701 atggtatgca tatacttaac cgggatattg tatcctctaa acccgatatt ctggtaaaac
    17761 tgaaagacga agcaaagtgg atgatactgg atgatacctc gctgttaaca ttgcaggtgc
    17821 gctatcctaa tggaactcta agaaggtttt attttaataa tgatacattg cgcttcaacc
    17881 ctgccgggca ggctccaaat cctgataata cagcgacaat caattttaaa ccttatttcc
    17941 aactggatgg cgaatatgaa atgataatta ccggtaagga taaaagcgaa aattcagcag
    18001 gaagtattga ataccgggta ttgttcgaag tgattaataa accgatgatc tcgaatatgt
    18061 tgaattatcc gaatccattt accacttcaa cttcgttcgt atttaccatt acaggaaatg
    18121 aagtaccgca gaatattaaa attgaaatta tgactgttac cggtaaaatt gttcgggaaa
    18181 ttaccaaaga tgagcttggg cctttgcgga taggccgtaa tataaccgag tttaaatggg
    18241 atggtactga cgagtatggg caaaaactcg ccaatggtat ttatctttac agggtagtta
    18301 ctaacctgaa tggtaaagca ttagataaat ataaatcgca aaacgataat acagataagt
    18361 attttaataa tgggtatggt aaaatgtacc tgatgcgtta aaaaatattc ctgattacga
    18421 aaagtcctgc acaagcagga ctttttttta cgccgctgat gaacgaattt tgctgcttat
    18481 atcatgttat tagttaaaac ctggataaaa agaaattttt cagaatggat ttattaccgg
    18541 aataaaagac cgggtaggaa atctgaaacg gataattggt tgaacgattt atgccgtagg
    18601 caaatttcaa aattatctga ttcttcttat aataatttta catatcatgg agaagatggt
    18661 attttactct atttgttacg tcagataaaa gatgtgccaa atgtatttgt tgatattgga
    18721 gctggagatt gtattaaaag taactgcgct aatcttgcgg ttcattacgg atgggatggg
    18781 gtttttattg ataaaaatga taaacaaatt gctattggca aacgttttta ccgtacaatg
    18841 atagaccggg gtagggatat caggtttctg gaacaaatga taattcctga aaatgtcaat
    18901 aatttgttgg agaattcagg tataggtggg gaaatcggat tattgtcaat tgatgtcgat
    18961 gggaatgatt attggatttg ggaagcaatt aaagtcataa aaccccgaat tgtagttatt
    19021 gaagcaaaag tggaatttgg gacgagaaat gtaattgttc catatggtga tcacaaccat
    19081 tattctgttg ataaaatgta taatggagcg tcggtagagg caatacgaaa actgggtaaa
    19141 atgaaaggat acaaaatggc cggctctaac aggcagggat acaatctttt ttttgtcagg
    19201 caggaagaaa atataacgga agaaagtgtt gactcagtct tatctgatcc ggaaacaata
    19261 caatcatttt accctgaatc attttataaa aagcataatt ttataacggt taattagtaa
    19321 taatggcgaa gattggtatt aatatagcaa ctggcagcct gcaaaaacag gaaatgattg
    19381 tcgggatcga tctgggcacg actaatagcc tggtagcaat cattcatcct gagacaggac
    19441 aacctgttgt tttgaaggag catgacggga tggctcttgt tccatcgatt gtgcattttg
    19501 gaaatgaagg gcaaatcgca gtgggtgatg aagcaaaaaa attcctgatt gctgaaccgg
    19561 agaataccat tttttcagtt aagcgtttaa tgggaaaatc gtacagtgat gtaaaagaac
    19621 attcttcttt tttcagttat aaaataatcg atgataatac tgaaagcctg gtgaaaatac
    19681 aggtaggatc caggttttat tcgccagtag aattatcttc ttttatatta aaggaattaa
    19741 aagcaagagc ggaacatata ttaaagacac cggtcactaa ggctgtaatt actgtgcctg
    19801 cttattttag tgatgcacaa cgacaggcaa cccgagacgc agggaaatta gccggactgg
    19861 atgttttacg tattgtgaat gagcctactg ccgcaagtct cgcttatgga atgggtttca
    19921 gcagagagga aacaaaaact attgccgtat atgatttggg cggggggaca tttgatattt
    19981 caatccttag aataaacggg ggcatttttg aggtactatc tacgaatggg gacacttatt
    20041 tgggaggaga tgattttgac agggagattg tgaaatattg ggcaacagaa ttgggtatta
    20101 ctattgaaga actgggtgtc cataagaatc ttgcacaaga gcttcgtctt ttggctgaag
    20161 aagcaaaaaa attacttact acttctgata attttaaggg taaagttgca ggacaagaag
    20221 ttaaattgac aaaaaagcag tttgaaaatc tcatactccc tctggtgctg agaactattc
    20281 aatcctgtaa gaatgcttta aaagatgcag atctgacaat cggcgatatt gatgaggttg
    20341 ttatggtcgg tggttctaca agagtaccgt tagttaaaca aaatgtcagc attttttttg
    20401 gaaagaatgt gcatgactct ttaaatccgg acgaggttgt agcattagga gccgctgtac
    20461 aggctgatat tttagctggt aacaataaag accttttatt acttgatata acaccacttt
    20521 cactgggcat cgaaacgatg ggtggattga tggatgtact gattccaagg aattctaaaa
    20581 ttccatcaaa ggctgctaga cagtatacta cccaaaaaga tggacaagga agtatgagaa
    20641 tagctgtctt ccagggtgaa agagatttag tgagggataa caggaaactg gctgagttta
    20701 atttaacagg tattccaggc atgccagccg gtttaccaaa ggttgaagtt tcttttttgg
    20761 tgaatgcaga tggtatactg gtggtaaaag caaaggagtt aagaagtggt gtagaacaaa
    20821 gcatagaagt gaaaccgcaa tatggcctta cagatgcaga agtggaaaaa atgctgatgg
    20881 attcaattgc acatgccagg gaagatatcc aggtcagagc tttggttgag gcgcaaacgg
    20941 aagcgaaaca gataatcgat acaacaactc agtttattct aaagaatagt aattttttgt
    21001 cggatgagga aatacaaaaa accagacaag caatggatgc tttatctgtc ttgaccacta
    21061 aaggagcaaa ggatgaaata caggcaggta tagaaaaact aaacgaaatt tcaaggcctt
    21121 atgcagaaag attaatggat gaagcgatca gtaaggcaat aaaggggaaa agtattacag
    21181 aataaaaata atgtaacgac tattttcaaa tattctttct ttaaaaagat caggttttat
    21241 tattattata tcattactta ctttatcaat aatcagtata attcttctat tcttccattc
    21301 aggtggtttt gatcctgttt tcaggaaatt tttctcttgt tctaatcttc gttctcgatt
    21361 tggagtatta taaggatgtg atacccatga attggcggag caataagcat tggatatata
    21421 atttagaagt acagcatttc caattagcag gtcatttgta gttttgttat tttttagaaa
    21481 ctcaagtact tcctttgtgt ctttggtgat atgtccttcc cattcagaag tatctttttt
    21541 tctcaggata tttactgtcc acagaatatt gtccaaaaga aaaaggataa taaatccttt
    21601 gaatagccat tttcttagct gactcttatt tgattcatta attaaccata ataaagcagg
    21661 taaagaaaaa agaaataacc cggcccagac ataaccacgg gtaaaatgaa tcggctgcat
    21721 tggccggata aaccattcat gtttggaaag cagaaatgca ataacagccc agcataaaaa
    21781 taaacgttga tgcgagtgcg ataggatctt agttgtttta ttaattcttg tagcaagaac
    21841 agataaaata ataacaatca ggtatgccgg aatggcaata cgtagactat aggtccagcc
    21901 ggcactgtat tgcgagtata gttgttgatg ctccggaaag ctattcaggt aaataagata
    21961 gtaccaggca tgaaaaaatg ttatgcatgt aatgcctgcc cagtagtaga ttggaatttc
    22021 cttattctta aacagtagct tttcaataaa aatccagcca ttcatgatta ataaatattc
    22081 aattcctgtg aatggatgtg aaataaaaag aaagaaagca gttacaattg cggcgctcca
    22141 ttttcgtttt aaaatcaggt agatatttaa taagaacaaa aaatggtagt atgcttccaa
    22201 aggaataaat aggtttctac cccagttcag accccaccat ccattggccg ggtcggctat
    22261 atgaatactg tcaagccatg atggcggttg ttgatcagaa aggaaaaaag cagcggcgaa
    22321 accagctata gcagttaatc caccacccca ggtaaatagt agtgaaataa gcgtttgatt
    22381 tctttgtcca ttcaaaagat gctgaattat ttgtattcct gtatagatgc aaaaaattgc
    22441 catgataagc ccaaaaagtg aaaaagtcaa tccggggtca agacctgctt ttatagcagc
    22501 agcaaaaaga aaattaattg gttggaaata tattttcgct gattgaggat ttccatcaaa
    22561 tgggttagag taaaaaatgg aaaagctatc ttggtcaaca tactgcctgg cataggacat
    22621 atatagtata ttctcatcaa cagtaaagcc ggttggttga aggtcattgc tatgatagaa
    22681 gtagtggtgt agcaaaaaaa gaattaccgg taatgctaat agcagggata ataatatcat
    22741 tctgttcctg ttcatcagcc ggcatttgga tttttagatt gtctgactaa tgatactgtt
    22801 ccgagtaata aaagagtgag gatagtgctg cacattatcc atatagcttt ctcagtatta
    22861 gccgggaaaa actcaaaaat aatcttatgc ttacctgtgg ccaatagggt acctataaaa
    22921 ctgagattgg ttttataaat cactgcaggt cttttgttaa ccgtcacttt ccagttatgg
    22981 ttgtaatttt gggtcaatac taggaacccc tgccggttca tttcagtttc aatttcgaac
    23041 ttatttgcag ataatttaat tatctgcgct ttaatgggtt tatcacagat tctatttgtt
    23101 actaaaactg tgtctgtaat tgcgtagtta cagctattct tcgttataag cttatttgaa
    23161 tccttcagac tcaaggtttg atccgcaata tatacaacag gaaaggaagc tatataattg
    23221 taaagcaact cattatttat aaactgttcc tgttggtgta aaaatgcggg ggagtttaca
    23281 gttttactta tgccgatttt tttgttatag aaatataaaa gagcaatttt attaaaatca
    23341 tcggttgcac ttttactgtt ttcgctaatt gtgttttcta atccttccgg tggatagcct
    23401 gcgggagagt tatttattag tgcatttatt tcttttggag ctgttttccc tacaaatgtt
    23461 gctggtaggg aaaattgcga cattataaat aggtttacaa tccatagaag cgagaatacc
    23521 cctatttttc ttcttgtttt cctgatccat agtgcaaagc tggcaatgaa tgccagttga
    23581 attattccat taataatgac agcatcactg agggatatat tatcaattat atcctttatg
    23641 gaggttgtta ttgcattgtt attaatgggt ttaaaaaata cagggatacc tgattggact
    23701 attgcgatta ttgttattag tagtataatc cctgtaatta ttattgcagg gaaatttaaa
    23761 gtacttatta tttgggtgtt tgaatagttt ttaatcaact cacttaatcc gggtgcagcc
    23821 aataagagta cagcaaagat aaaaaacaac cgtacctggg atggatgcct gaatgtattc
    23881 attagcggca ggtaatcgta acatagcttt ctaagcggag taagatcacc caggctgaat
    23941 agtacagcaa aaagagccag gccaatcagt aaaatattct ttcggtttgg ccttggaggg
    24001 aaagtaaata gtattagtag ggggaatata ccagtatata cattcctgaa gctgatatct
    24061 gttgatgaac ttatgtcagt agcgcttatt gaggagggga aaagtactga taagaaatgt
    24121 tgccactcaa acgaattaat gactgcttcc gtatagctga ccccgcttcc tctctggtag
    24181 taaggtaaaa gatcaatata ggagactatt gccggcagag ataatcccac gaaaacgagg
    24241 ataagcaaaa gatgttgtaa ataaaataag ctccatttag cagtctctga ctctttaagt
    24301 attagttttc ttacaattgt aataatgaaa agaaacaata agatataaca ggtcgtgata
    24361 aaaaaagaag ggtagccggc agtaaataga aaaaataatg caattccagt tttgattgca
    24421 gagccatttc tagccgtctt aagtagtaga gtataataat gtaccacaaa cggaataaaa
    24481 gcggcagaag ccagccagtt tataagttgt ccgctgagca taaatccact caacatataa
    24541 cttaccgcta tcaaaaacgc tgcatgtgta tgtactgtca ggcgattgac tagtttatac
    24601 attccggcac cggcaaggaa tatgtacagc agattttccc agtgaaataa agtgatatta
    24661 tacctgctta tgcatgaaaa tagccatacg aaagggttcc acacgccact ttgcatatcg
    24721 ccatagatag gattgccaag ataaatatag gggctccaga atggccactc cccatttctt
    24781 attgcttcac tgatattaaa acgatagggt aagaagtaat gaatggcatc attttttaca
    24841 ctgaatatat taaaagttaa tggccagtag gtgagcaggc aaatgattaa caagaaaaga
    24901 tagttcctgt attgatcatg tttttgctga aaaatgcctg taatattcat gtagttttcg
    24961 tctgattgac agctacaaaa tagataaaaa aatcattaaa acactcttat tgatttttct
    25021 cattggtgag aagtataaac atttgcgatg ttttggcaaa aggccttctt ataaaatcaa
    25081 aaagggcaaa taatgcgcta acaggagcga agagccagtg aaaaattata gggttttgag
    25141 ctgaagtgtg gattattctt ttgcggtgta aagcaactag aatgctgaat gcccagaaag
    25201 gagccccctg tgtatagttc agctttttga tatttagtct tgttgattgc agtaaaatcc
    25261 taaaactctt ttctgagaaa ataacccagt gccgggggct gtgaagtccc ccccagtagg
    25321 ttcttcgaaa aattcttgca tccaggctat cagtattcgg agttttaatc ataattatac
    25381 ctccgggatg tagcagggtt tctgcttttt gtagaacagc taaaggattt gctacatgct
    25441 ctatcagatt aagcattaaa atcaaatgaa atctcttgtc tgtattaaat tcttcaatag
    25501 ttcctttaaa atacgcatgt ccattctgtt cggctacaat acttgctttt tcatcaatat
    25561 ctgcaatttg tgtaaagtca atacggttct cggttttttt taatacatcg agtatccagc
    25621 cagttcctcc gccaacatca agtacatcaa tcttagcact gttcagttgg gatagaattt
    25681 ttttgaaaag ccttttatcc agccattctt ttattttaac gactatgttt tttgaaacag
    25741 tgacgaacga ataatagttg gaaggatata tttctttaag ctggctaaga ggtacgggat
    25801 gtataaaaat agttccacaa tcagtgcaat gataatatgt aaaatcttct tttacagaat
    25861 gatactcata gtctttagcc acattccata tgctggtatt tttttgatgg caaacagggc
    25921 aatatagatt agatgaattc aaggagcggc caggagcttt ttaagtactg aggagtagga
    25981 ggtttttgaa gagtggatta tttccgcagt ttttttatta tacaggagaa aatcctcaat
    26041 atcttttaac cgtgtataag agatctgtgg atcccaatga tgtatcatgt gtcgggtgaa
    26101 tcctgctgca ccaaaactac tgcttaagat tgtatgaata aataatctgc tgattttccc
    26161 atgttgaatt ttattgaaat tagtggcatg atccgccaat acgtccctgt gttccaatat
    26221 ctgccttatg gtggcaaaaa atggaaaaaa aaatccaaat cccagaatcc atgtaattgc
    26281 aaaagtccaa tgaccagtga gaaaggtaag tgccagaata attgcatgta acaagaatcc
    26341 cgcaatcaac ataatactgc tttctttttt ttgttcctta gttaagacat tgttattctc
    26401 ttttttcagc atagttctga gtaaatgaat acccgtaagt gtttccagga taaatagttt
    26461 tgtcagggga ttaaagtatg aggtttctgt atcctgcgga gtgcccaggt aaagatgatg
    26521 ttgccagtga atttttctat atgctttcat agaaagtccg aatggaaggc aaaggaagaa
    26581 agttgccagg ctgtcattta tctttttgtc cggatgtaca ttgtaatgcc ctgcctcatg
    26641 aataaaaagg gctaaataag cagcaatata tccaataaga atactacctg cagaaatata
    26701 cacccaccat aaagatggat aagtttgatt caaaagaata acaaggcagg aaataagcaa
    26761 taaaaagaaa taacccttta taatatctga aattactttt gtataccggg ggagcaatgt
    26821 tgttctgaaa acagcataac ttataccggt tttatctgtt acttcatcaa aagaaattcc
    26881 ttcgaggtta ttttgcatag ttgttattgc aaaactataa aaagagaatt ttattgaggc
    26941 tgttcttttt cccagacagc aattatactt ttcccaaaac tgttaaacaa taatttatcg
    27001 gtgattcttg aagctggtat tatccacctg tcccaaaaca gaacttgttt gattgtagga
    27061 tatttctgac gaagcaaaaa cttattcacc aatgctgcaa aatacccggt tgtgtcgtaa
    27121 tacctgcaat tcagcatttt cagcgtagcc ggtgtcaatt gccggagcat cttcttatta
    27181 tatctcctgt aatggccaat ggctttgtca aatggattat aaaggaattg aaaagctggc
    27241 gaaagtacaa ttaaatgccc gccattactt aataaggcgg ccgctttttt tatttcctct
    27301 ttgtctgatt caatatgttc cagtacatct atatatagta tggtatcaaa tatttctgta
    27361 agttgatcga ttgttcctgt ttgtaattta caattagcag gaagttcttt ttcagaaatt
    27421 tttttatcaa gtatttcact catctccttg tcaggttcca gcagcaacca tttagcataa
    27481 caaccattgt taagaagtaa tgtggtggca ccaattccgg caccggtttc cagtacattc
    27541 ccgttgatat aaggagtgat ctgttgggaa aagtattcct tccagttctt tgcatattga
    27601 aataagacta actcaccgcc ctggtatttg aaggaatctg tcatttatta aacggtttca
    27661 acagctccgg cataatcctt ataatgtttt gccggaataa atttccgctg agattgtgat
    27721 gataaataaa gaaaaatggt gaataaagaa agtagaaaac tctgtaatag aattattagc
    27781 attgctgaaa taacagttga agtccatccg ggaatagctc tgtcagtaaa cgtttttatt
    27841 cccaaaagcc caagtattac tattaaagaa ataccaatga gcaaaaggga aaatattaat
    27901 agccggctgg caatgatttc aatgaatacg gcaatagcac caagtccgtg aatcagtaat
    27961 gaagtaaaac tcatcttaga gctgccatca tatctcttgc ctctatgtgt gttaacaagg
    28021 atatatggta acccggtctt tataatacct cctgcgatat gactccagat ctcactatag
    28081 aatacaactt tatcgagcaa caattttggc attactaaaa aattgccaaa agttattttt
    28141 ttacctgtaa agaggtgaaa taccattttg tatatccggt aaaaaagctg aaacttgttg
    28201 ctttcctggc gggattttct gtgagcaaaa atgatcttac caggattctt atcagcttct
    28261 tccagtagtt tcactgcatc ttcaggacgg tcttcaccgt cactatccat taccagcacc
    28321 ttatcgcatg aaagattttc tttaatatat gccagcccga tggcaatcgt tttttgatgt
    28381 ccaatatttc gctgaagttg taaaatggta cccgggaaag tggaatcttt gctgatattt
    28441 attgattctg ttgatccatc attaacgatt agtacagaaa agttataatt atttagatct
    28501 ttcaatgaaa cagacagttc attcaaaagt ttatttactg atttctcatc attgtaaacg
    28561 gggataagta ttattatttg ttctgccatc agtttagggc ttaaagatac aatcaaaaaa
    28621 atgataaaga aatgttgttc tgcttatata aaagtatcct tcaatttcag aaaatatttt
    28681 tcaaaatatt ggaaactgat ccagctaata aatattgctg cgagggtagc taaaagtgaa
    28741 acagtaaaag gcaataaaga gccacttacc tgttttgata atacaggaaa cagggatggg
    28801 gctaacagga tatagattgg ccagtgaaaa atataaaagc catatgaaat tctgccaaaa
    28861 aacttaagta atggaatgtt gaataaaagg ttgatcaatt ttgttttccc agatacagct
    28921 tcattaacca ataatccaaa tatcattgca atggtggtgt agccaactaa agccagataa
    28981 gggaatgaga aatgatagct gcggttaata aaaaagaatg caaaattcaa tactgaaaaa
    29041 aataaaacaa tttctgtcgt ataggtttta agaaaattac tgtttattct atgcaacagc
    29101 gctatcgtac agccgataca gattccatca atacgggtaa aagtgtacag gttaaaataa
    29161 gccagatctg caacctggtt attccaaatc cataaccgca aaaaaaggac acctgctaat
    29221 aacgatgaaa taaccagcaa aaggtattta ggtttacgga taatcataat gacaagaggc
    29281 caaagcaaat aaaattgctc ctcaacagcc agagaccaga ggtgattcag tgcattggtt
    29341 tgatcaggat ctttgaaaat atacaaccag ttttgcaaat aggtccaaag ccatatctgg
    29401 ttatcggtat agtactttat attaagttca atgtttaacc gtggtataat cagcaggaag
    29461 acaatcagga atagataata caaagggaaa attctaagta tccggcgtat ataaaaattt
    29521 ttcagataat tttttgttcc aaccgttttc agcagaatat cggtaattaa aaaacccgaa
    29581 agcacaaaaa acaggtctac acccagccag ccgaagaaga agtagtcaat aaagccgaaa
    29641 ttatggtaaa ccaccaccag taagatagca agtcccctaa ggccatccag ggcagggtaa
    29701 tattgcctgt taggattttc ggggttcatc aggtatgaaa taggttagta attagtcttt
    29761 caaagataat cttctgagca gatactttcc aatcatatca aattcaaggt ttacctggtc
    29821 accaatattt atattcctga tattggtgtg ttcaaaagtg tagggtatga tagcaacgtt
    29881 aaaggttttc tttttcacat tgaaaacagt aagacttatt ccattcaggc atattgaacc
    29941 cttttcgata accagggcag caaatttttt gggaaatgca aagccaaact cccagctacc
    30001 ttctttttcc agcttgctga tacaggtccc ggttgcatct acatgcccct gaacgaggtg
    30061 accatcaata cggtcattta gctgaagaca acgttcgaga ttaactagag agccctcctg
    30121 ccattgtccc aggttcgttt tctgaattgt ttcatcaata gcagttatcc ggtgccggtg
    30181 attactaatt tcctccacag tcaggcaggc gccattatgg ctcatgcttt gatcgatttt
    30241 tagttggtta gaaatagggg attcaaccca aaaagttcgg ttagaaccat tggaaatgac
    30301 ctccttaacg gtccctgtgc attctataat tccagtaaac atggcagcaa gttaaatcat
    30361 ttcttttttc attttctttt ttcagaagaa ccgcctatct ttgccatcct aaaaaaatac
    30421 cgaaggaggt atataagtta tgctgataat agactcaaaa gattgcgaaa acattgacaa
    30481 ggcgttaaaa aagtacaaaa agaagtttga gaagtcaaaa gtacttcttc agcttcggga
    30541 acgccagagc ttcaccaaac cctctgtgaa gcgtcgtggt gaagtcctga aagcaattta
    30601 caagcagaat ttggccagcg gaaagatcga aagctgatta tagctgcccg gtaatattat
    30661 attataagat agctgcaaag tttaatagct ttgtagctgt ctttttttat gcctgtacca
    30721 gcccgtcagc gtatacaatc ctttcttgat tacctcaagt ttgagagaag atactctcct
    30781 catacattaa catcatatac tactgatctc attgattttt ttgattacct ggaatcgcac
    30841 tttgacatag tagatgtaaa ggcaattaat tcaggtttta tccgaagctg gctcgccggc
    30901 ctgaaagaga aaggccttac ctcaaaatca gtaaaccgta aaatatccag cctgaaatct
    30961 tttttcaaat acctgttgaa aacgggagaa attgaaaatt ctcctatgaa caatgtgatc
    31021 agccctaaaa taagtaagcg gctgccggtc tttatgaagg aggaggatac aaaaaaactt
    31081 gttctggcat taaatcagtc tgctgaggat tggaaaagcc ttaatgcaaa aatgctgata
    31141 actatttttt atgctacagg tatgcgatta agtgaactca taaatctgaa agaaaaacaa
    31201 attgatttca gcaggggaca tataaaagta ttgggaaaag gaaataaaga aaggataatt
    31261 cctgtcagta aggaagtgat tgaagtaatc ggggaatatc agcaattaaa aagaagggac
    31321 tttgtgggaa cagaagatta tttattaata acggaaaagg gcaataagct ctatccaagg
    31381 tatgcctggc aactggtgaa ccaggttctt ggacaagcca gtacacttga taaaaaaagc
    31441 ccccatgtgc tccgccacac ttttgctact catttgatga ataacggagc tgatctgaat
    31501 gctgtgaagg aactgctggg gcatagcagc ctggcatcta cacaggtata tacacataat
    31561 accattgcaa agttgaaaga tattcataaa aaggcccatc cgaaagctta gaaattctct
    31621 cgtttgagta aaaaaaatta tgattttttt acttggtgta aaacttcttt tgttgtaact
    31681 tgatcatgat aaaaggaccg aacccttaga aatgtttttc cagaaaagtt ctttatttat
    31741 tgtttcacag tttaaaaatg atgaccatga atgtaaaaat tcaaactgtt catttcaacg
    31801 ctgacagcaa gttgaaagag tatgtaaacc gaaaacttga aaagctgacg gtgtttagcg
    31861 atcgcatcat cgaagtaaat gtttttttga aactagacaa tgtggcgcat tcgattaaag
    31921 acaagattgc agaaatcaga gtacatgtgc cacgtcacaa tttttttgtt aaagcaacca
    31981 gtaaatcatt tgaagaatct tttgaaagtg ctttcgactc tctcgtaagc cagataaaac
    32041 gaaaaaaaga aaaacaagcc gcttaaaaaa ttcaagacct ccccggaggt cttttttatt
    32101 tcttgatttt cagcagaaaa atctcttcta aaatttttgc aatatctaaa ttcccttacc
    32161 tttgccttcc caaaaattgg gggtagttct ttattgcctg ttttgccgca ttatccagct
    32221 acggcgaaat taaaattgcc accttagctc agctggtaga gcaactgatt tgtaatcagt
    32281 aggtcgccag ttcgattctg gcaggtggct caaggcgggc aagtagccaa gtggccaacg
    32341 gcaacagact gtaaatctgt cctcttcgga gttcggtggt tcgaatccat ccttgcccac
    32401 tttatcaatc ttaattaaaa ccagggttgg aaagttcttt ttttgaaata gcatcagcgg
    32461 gagtagctca tttggtagag cggtagcctt ccaagcttca ggtggccggt tcgagcccgg
    32521 tctcccgctc aaaaccgcca tagctcagcg gaggcggatc tgataagacc agccgttgta
    32581 gctcagtggt agagcacttc cttggtaagg aagaggtcaa gggttcaatt ccctttaacg
    32641 gctcaaattt tagcaggttg agggaaaaat gacctgttga tttaagggaa agggagtagg
    32701 gagtcaatgt aatgaaagct aatagtagta atacctgcgg cttcataata aaaaacaata
    32761 attgtaaaac acaactttaa aaccgataac aatgtcaaaa gagaccttta agagggacaa
    32821 accccacgtt aacgttggta ccattggtca tattgaccac ggcaaaacca ctttgaccgc
    32881 cgctattaca accattcttg cttcaaaagg tatggcaacg gcaaaaaaat atgatgaaat
    32941 cgacgctgct cctgaagaaa aagaaagggg tatcactatt aacacggctc acgttgagta
    33001 ccagacggca aaccgtcact atgctcacgt agactgtccc ggtcacgctg actatgtaaa
    33061 gaacatgatt acaggtgctg cccagatgga cggcgctatt ttagttgtgg ctgctacaga
    33121 tggtccaatg cctcaaacaa aagaacacat tctgctggcc cgccaggtag gtgttcctaa
    33181 gattgtggta tttatgaata aagttgacct ggttgatgat cctgagctgt tagagttggt
    33241 ggaaatggaa atccgtgacc tccttacatt ctatggtttt gatggtgcta atacgccaat
    33301 catcaaaggt tctgctatta aagccctcga aggtgaagca gaagctgtaa aacaagtaga
    33361 agagctgatg gaagctgttg acacatacat tccgttgcct cctcgcccgg ttgatcaacc
    33421 tttcctgatg tccgtagaag acgtattttc catcaccggt cgtggaacag ttgcaacagg
    33481 tcgtattgaa agaggtcgta ttaaagttgg tgagccagtt gaaatagttg gtttgattgc
    33541 agaaccgctg acttcagttg taactggtgt ggaaatgttc cgcaaattat tggacgaagg
    33601 tgaagcagga gataatgctg gtttgttgct tcgtggtatt gagaaaactc aaatccgtcg
    33661 tggtatggta atcgtaaaac caggttcaat aactcctcac actgaattca aagcagaggt
    33721 ttatgttctg agcaaggaag aaggcggacg tcatactcca ttcttccaga aataccgtcc
    33781 tcagttctat ttccgtacta cagacgtaac cggagaagta gagttgaatg ctggtactga
    33841 aatggttatg cctggcgata acacatcact tactgtaaaa ctgatccagc caattgcgat
    33901 ggaaaaaggt ttgaagtttg cgattcgtga aggtggccgt acagtaggag ccggacaggt
    33961 tactgagatt ttaaaataat cttttatctt tgatacttca aaagtcattg gtcgataggg
    34021 ttttccgaac gaccggtgac ttttgattct tacgggcata gttcaatggc agaatagcgg
    34081 tctccaaaac cgttgatggg agttcgaatc tctctgcccg tgcaaattga tttttatgaa
    34141 caagattaca acctatttca aagaaagcta taaagagctg gccgataaag tgacctggcc
    34201 tacctggcca caacttcagc aatcaacaat gattgtattg gctgcaacta tcgttttcac
    34261 ctttatagtg cttgcaatgg attttgtggc aggtaatgga ttgaagttta tctacaaaca
    34321 actattctaa tggaagcaac tacaaacaca gatacacaac agcaggaaac caaatggtat
    34381 gtgctgcgtg ttgtaagtgg aaaagaaaaa aaggttaagg aatatcttga caaagagatt
    34441 tcccgtggtg gctggagtga aattgttaaa caggtattcc tgccagtaga gaaagtgtac
    34501 aaagtagcca gtggaaaaaa ggtaatgaga gaacgaaatt attatccggg ttatgtgatg
    34561 attgagatta cagaaggaaa attaagtgat gatctcaggg atgttatcaa aaataccaac
    34621 agcgtaattc attttttagg aaaggaaaac cctattgccc ttcgtaaagc tgaagtgaat
    34681 aagatgctgg gtaaaatgga tgaaatggcc gaagctggcg gagtaagtat gagtgagcca
    34741 tttattgttg gagaaacgat caaaatcata gaaggtcctt tcaatgattt taatggtgtg
    34801 atagaagaag tgaatgatga aaagaaaaaa ttgaaagtaa cagttaaaat atttggacga
    34861 tctacaccag tagaactgaa ttatatgcag gttgaaaaaa taaactaata aagtttttac
    34921 atgctattta gctaagccac ccttacgggt ggcttttttt ggcagattaa gtttttatag
    34981 agttattaac cccttgttgt taatttttaa tactcatagg gtttaggagt aaaaaaacat
    35041 ccctaaaacc tttgcccata aaggatttac ccctaccttt gcagccccaa ttagttcttt
    35101 agaaacccca ggtaactggg aatgaggaat ttgggagacc gagtagtcaa aacgaaaggt
    35161 cgcttgaacc aataaatcaa agaaaatggc aaaagaaatc agtggctttg taaagctaca
    35221 gtgtaagggt ggccaggcca accctgcacc tccaatcggt cctgcgctgg gttccaaggg
    35281 tatcaatatc atggaattct gcaaacagtt caatgcaaga acacaggaca aaatgggaaa
    35341 agtaattccc gtattaatca cagtttatag tgacaagagt tttgattttg ttctaaaaac
    35401 tcctcctgca gcaattcagc tgatggaagc cgctaagatt caaaagggtt ccaaagagag
    35461 taaccgtgca aaagtcggaa aagtaaactg ggaacaggta aaggctattg ctgaagacaa
    35521 gatgcccgac ctgaactgtt ttaccgtaga aagtgccatg aaaatggtag ccggtactgc
    35581 tcgtagcatg ggcttgaatg tagaaggtaa agctccgtgg gaaaattaat tcttgaatcc
    35641 aaaaacgaat tacaatggca ttcattagta aaaaaagaaa agttgctgat acaaaagttg
    35701 acgcaaacaa agcatattca ttaaaagaag catctacatt ggtgaaaagt atcactacct
    35761 gcaaatttga tgcttctgta gatctgcaca tccgtttggg tgttgacccc aaaaaagcag
    35821 accaggctat ccgtggtaca gttaccttgc cacatggtac tggtaaaaca aagaaagtat
    35881 tggtgctctg tactcctgat aaagaggctg aagcaaaagc tgctggtgct gactatgttg
    35941 gcctggacga gtttgttacc aagattgagg gcggttgggt tgatgtagat gtgatcatcg
    36001 ctacaccttc agtaatgcct aagattggta aactgggtaa agtgttaggt ccccgtaacc
    36061 tgatgccaaa tcccaaaact ggtacggtta ccaatgatgt agccaacgct atcaatgatg
    36121 taaagggtgg taagatcgct ttcaaggttg ataaagtggg tattatccat gcttccatcg
    36181 gccgtgtaag ttttgctcct gaaaagattg ccgaaaacag ccaggaattg ctcaacgcaa
    36241 tcatcaaggc taagccatct tcctcaaagg gtacttacct gaggggtatc agcatggcca
    36301 gtacaatgag tcctggtatt gctgttgaca caaaagcatt tgttcactaa tccataaagt
    36361 gattataaac tttttatcat gacaaaagat caaaaaaatg aagtgattga attgctgaaa
    36421 ggcaaattct ctcaatacaa taacttctat gttgcagata cagaatccct gactgtagca
    36481 caggtgggca aattacgccg tgcatgtttt agcaagcagg tggaaatgaa agttgctaag
    36541 aacactctca ttaaaaaagc attagaatca ttagacagtg aaaaatatgc aggcatgtat
    36601 gattccctgc ataaagtaac agctcttatg ttttctgaaa acccaaaaga accagctctc
    36661 atcatcagtt ctttccgcaa agaaagcaat ggtgaaaagc cggtattaaa agccgctttt
    36721 atcaatggcg atgtatacat gggtgataat caactgaaag ctctcacaca gattaagact
    36781 aagaatgagc ttattggtga agttattggt ttgttgcagt ctcctgcgaa aagagttctg
    36841 gctgctttat tgcaccatca cgagcagaaa gcgggcagtg ctgaagtagc agcagcagaa
    36901 taatttgagt aataagctac gagctatctg gtatgagttg cgagactaag tatcgaaact
    36961 catagctaaa aattcgaagc tgtactataa aacaattttt ttaaaaccgc cgtcggatcc
    37021 cgcataacgg aaatgaagac ggtaaaaatt caaacaaaat ggcagacgta aaagcattag
    37081 cagaaggcct tgtagcctta acagtaaaag aagttcagga actggctgac tttttaaaat
    37141 cagaatacgg tatcgaacca gcagcagcag cagttgtagt tgcagcaggt ggtggcgaag
    37201 gtgcagcagc agctgaagag aaaactgcat tcaacgtagt attaaagagt ggtggtgcat
    37261 ctaaactgaa cgtagttaag atcgtaaaag acttaaccgg tttaggtttg aaagaagcaa
    37321 aagacctcgt ggacggtgct cccaagaaca tcaaagaagg tgtatctaag gcagaagcag
    37381 aagatatcgc agctaagctg aaagaagctg gcgctgatgt tgagatcgct taatttcttt
    37441 actgaataat tttaaaaccc ttcgccttgc ggagggtttt ttattgaatt acaaggtaag
    37501 gtgaaatttt attatatagc tcatccgtaa ccgccatcac tttttttata tcctctactc
    37561 ttgaaaacgg cccatgctct tttcggtatg ctacaatggg attggcaatg ccccatttga
    37621 tatatgggtg cgcctttaat tcatcaagtg ttgcagtgtt gatattaagt tttcttatag
    37681 tggtattttc cactttcagg tattgcttta ttttttgaaa agtagaatcg ggcaatccaa
    37741 aggtttcgcc aacttgagaa acagaataga acccccctaa cttatctctg aagtttacaa
    37801 ttcttgctgc cagtttactg ccgattccgg gtaaagctat cagcgcagtt gtatcagtct
    37861 cattaatatc aataattgtg taatagggag gtgtgtactt ttttgaagga tagctttctt
    37921 ttggagcctc gttatgagca taatcctgcg tattctttga ttgtaaatcg gtttctatcc
    37981 tgatgtaggg agctattcgt ttatattcat ctggaaataa gccataaatt ttttgtagat
    38041 cttcctggtt tttaaaacgt ccgcctttgc tcaggtagtt ttgaatagtt ttgattgttt
    38101 tgtctctcaa ccctaatctt tgccatcctt ctggtgaaag tgtattagga tcgaagtaaa
    38161 ataggttaac ttttgttttt ccgtaatagt cgctggttga acggtcgtat tggtaatggc
    38221 tactgttatc atcggcaaat ttcccgggtt cgtcctgtgc ttctgtttct ttttgttcca
    38281 gtttcagcat ggaagtaatc cagctagtat cagaactgtt ttctttagga agggactttc
    38341 cttttgaaaa cgcatttgga aggaaaaagg cacttagaat aacgcctaaa atgacagtta
    38401 ttcctatcct gtcttttcgg ctgaaattga gataatcatc agtaaattct ttccaattca
    38461 tagccttatt tcgggtcaaa ataatgcaga ttcttgtcag aaaaaatggc cacttagtgg
    38521 tatttctggc cgggttattg tgagcaaatt tttcttggct aagaactata aatccctacc
    38581 tttgcaatcc tttattttgg ccaaacatat tttgacatcc caaatcatgt gtaagtaatt
    38641 gaatttcact gactttcatt ttttctaaaa tgagagcttg aatatgattt gtgtgtcctc
    38701 aacgttgtaa aaaccggttt tacgcatatg tcttcaacaa aaatgaactc aagagtggat
    38761 ttcggtaaga ttaagcacct ggctgaaact cccgatcttc ttgaagtaca gattcaatcg
    38821 ttcaaggaat tcttccagtt agaaactact ccggacaagc gaaacatcga aggtttgttc
    38881 cgtgtgttta aagacaattt tccgattacg gatacccgaa atattttcgt attggagttc
    38941 ctggattatt tcatcgatcc accccgttat acaatcgaag agtgtatgga acgtggattg
    39001 acttacgctg tgcctctgaa agccaaactt cgtttgagct gtaatgatga ggaacacgtc
    39061 gatttccaga cgattgttca ggacgtattc ctgggtaata taccgtatat gacaccccgt
    39121 ggtacgtttg tgatcaacgg agcggaaagg gtagttgttt cccaattgca ccgttccccc
    39181 ggtgtattct tcggtcaatc cgttcacccg aacggaacga agatttactc tgcaagggtt
    39241 attcctttca agggtgcctg gatggagttt gctacagata tcaacaatgt gatgtatgca
    39301 tacatcgacc gtaagaaaaa attccctgtc actacgttgt tacgttctat cggctacgaa
    39361 acagataagg acatactgga actgtttggt atggcagatg aagtaaagac tgataaaaag
    39421 accttaagta aatatgttgg taaacgtcta gcagcccggg tattgagaac atgggtcgaa
    39481 gattttgtgg atgaggatac cggtgaagtt gtttctattg aaagaaacga aatcgtaatg
    39541 gagagggata cggtactgga tgaggaagca attgaaacca tttctgaaat ggaagtgacc
    39601 agtgtgttta ttcaacgtga agatgttggc ggtgattatg cgatcatcta caacacactg
    39661 aataaggata cttctaacag tgagttggag gctgttcaat tcatctatcg tcaattacgt
    39721 ggagcagatg ccccggataa cgagacagcc cgcggtatta ttgataaatt attcttcagt
    39781 gataaacgtt acgatctggg cgaagtgggt cgttataaaa ttaaccgcaa gctgaatgta
    39841 gacttgccgc ttgagaagaa aacacttacc agggaagata tcattctcat aatcaaatat
    39901 cttgttcgtc ttactaatgg taaagccgaa atagatgata tcgatcacct gagcaatcgt
    39961 cgtgtacgta cagtaggtga acaattatat gctcagttcg gggtaggact tgcccgtatg
    40021 gccagaacta tccgcgaaag aatgaatgta agggataacg aagtgtttac acccgttgac
    40081 cttattaatg caagaacatt atcttctgtg atcaattcgt tctttggaac atcgcagtta
    40141 tcccaatttc ttgaccagac aaacccatta tcagaaataa cgcataagcg tcgtatctcc
    40201 gcactcgggc caggtggttt gagtcgtgaa agagctggtt ttgaggttcg tgacgtacac
    40261 tattctcact atggacgttt gtgtacgatt gaaactccgg aaggtccaaa catcggtttg
    40321 atctctacac tttgtgtgca tgccaagatc aatgatatgg gcttcattga aacgccttat
    40381 cgtaaagtac acgaggggaa agtgaacatg aaggaattaa gtttcctgag tgccgaggag
    40441 gaagacctgg ctaaaattgc acaggcgaat acacctcttg atgagaaggg taatttcaaa
    40501 gaagagaaaa tcgtaagccg tgagaccgga gattttccga ttcttgataa aaatgatgtg
    40561 gaattcatgg acgttgcgcc aaaccagatt gttggattga gtgcttcgct gattcctttc
    40621 cttgaacatg atgatgctaa ccgtgcgctg atgggttcga acatgcaacg ccaggcagta
    40681 ccactgttgc gtcctgatgt acctatcgtc ggcacaggat tggaaggtaa agcagcgaga
    40741 gatgcaagga ttcagatcca tgcagatggt aatggggtgg tagagtttgt agatgctaat
    40801 gaaatccatg ttcgctatga cagggatgag gctgaaaaat tggtaagttt cgaagatgat
    40861 ctcagagttt acaggttaac gaaatttatc aagaccaacc aggaaacctg catcaacctg
    40921 agaccagcgg ttaagaaagg tcagaaagta aaaaagggtg attttttaac tgaaggatat
    40981 gcaactcaga atggtgagtt ggcacttggt aaaaatctta aagtggcatt catgccatgg
    41041 aaaggttaca atttcgagga tgccatcgtt atcagcgaaa gagtagtaaa ggaagatttg
    41101 tttacttctg ttcacatttc tgaatatgaa ctggaagttc gtgatactaa actgggtgaa
    41161 gaagaactga cacctgatat tcctaacgta tctgaagaag ctacaaagga tctggatgag
    41221 aacggtatca tacgaattgg tgcacaagtt aaagagggtg atattttaat tggaaagatt
    41281 acaccgaaag gtgaaagtga tccaactccg gaagaaaaat tattgcgtgc tattttcggt
    41341 gataaagccg gagatgccaa agatgcttca ctaaaagctc cgaacggagt ggaaggtgtg
    41401 gttatcggca agaaattatt ccagcgggct aaaaaagata agaacgccaa agtaagagag
    41461 aaagcagcca ttgagaaatt ggagaagatg catgagaaaa atgaaactga tctcatggaa
    41521 gtgctgctgg aaaaacttgg tatgctgtta aaagatcatg tttccgcagg tgtaggtaat
    41581 aattttggtg aaatacagat tggcaaagga gccaagttta ctgaaaagaa tcttcgcggt
    41641 cttgattatg ccaacattaa ccccctagga tggactggtg ataaaaaaat cgatgatcaa
    41701 attaatacac ttcttcacaa ttataatatc aagttcaacg aagagttggg acgttataag
    41761 agagagaaat tcaatatcag tattggtgat gaattacctg caggtgtatt aaaactggct
    41821 aaagtttatc tggctgtaaa acgtaagttg aaagtaggtg ataaaatggc cggccgccat
    41881 ggtaataaag gtattgttgc gaagatcgtt cgtcatgaag atatgccgtt ccttgaagat
    41941 ggaacaccag ttgacatcgt tttgaatccg ttgggtgtac ctagtcgtat gaatcttggt
    42001 cagatatatg agaccattct tggatggaca ggtgagaagt taggtttgaa attctcaacc
    42061 ccgatctttg atggtgctac tgttgaagag atcgctgaat actgcaagca ggctgacatt
    42121 ccaatgtatg gtcacactta cctgtatgat ggtgagacgg gtgaaaggtt ccaccagaaa
    42181 gccactgtgg gtattatcta tgtgattaaa cttcaccata tggtggatga taaaatgcat
    42241 gcccgttcta tcggaccata cagcttgatt acacagcagc cgctgggtgg taaggctcag
    42301 tttggtggtc aaaggtttgg tgagatggaa gtgtgggcac tggaagccta tggcgcatct
    42361 aatattcttc aggaattgct gacattgaaa tcagatgata ttatcggccg tgcaaaaaca
    42421 tatgaagcca ttgtgaaagg tgaaaatgtg ccaagagccg gtgttcctga atcattcaat
    42481 gtattggtgc atgaactgag aggattagga cttgacctga agtttgaata aagggtcagc
    42541 cggagaatga ccatagtttg caaaggtgaa ttatggtaga tgaactaaat cagaaatcta
    42601 aaatctaaaa tcgtcaatac gattatggct atcagaaaag aaaatcgtcc caaagcggct
    42661 ttttcaaaga tcacaattgg tcttgcctca ccggatacta tcctggaaag aagttatggt
    42721 gaaattttaa agcccgaaac gatcaactat cgtacataca aacctgaaag agatggcttg
    42781 ttctgcgaaa gaatattcgg accggtaaaa gattatgagt gtgcttgtgg taagtacaaa
    42841 cgtatccgtt ataaaggcat cgtttgtgat cgttgcggtg tggaagtaac tgagaaaaaa
    42901 gtgcgtcgtg aaagaatggg gcacattaag cttgtggttc cggttgttca catctggtat
    42961 ttcaaatctt tgccaaataa aattggttat ctgttaggtg ttagttctaa gaaattggag
    43021 actatcgttt attatgagcg ttttgtggtg atccaatccg gtattcgtgc tgacaaagga
    43081 caaaaccccg gtgatctgtt gacggaagaa gagtatttgg atatactgga tacattacca
    43141 aaagataatc agtacctgcc tgatgatgat cctaataagt tcatcgctaa aatgggagca
    43201 gaagcagtgc atgatatgct ggcaagaatt gatcttgacc agttatcttt cgatctgcgt
    43261 aacgctgctg ctactgaaac ttcacagcaa cgtaaagctg atgcattgaa gcgtctgagt
    43321 gtggtggaag cgttccgtga tgctaataca aggatatcta accgtcctga gtggatggta
    43381 atgcaataca ttcctgtaat cccgccagaa ttgcgtcctt tagtgccgct ggatggtggt
    43441 cgttttgctt catcagatct gaatgattta tatcgtaggg ttattatccg caataaccgt
    43501 ttgaagagat tattggagat caaagcacct gaagtaatcc tgcgtaacga aaagaggatg
    43561 ttacaggaag ctgttgactc attgttcgac aacagccgta agtcaaatgc tgtaaaagct
    43621 gaaggcggac gggcattaaa atcactcagt gatgttttaa aaggaaagca aggtcgtttc
    43681 cgtcagaacc tgttgggtaa aagggttgac tattctggtc gttctgttat cgtggtaggt
    43741 cctgaattga aattgcatga gtgtgggtta ccaaaagata tggcagctga attattcaag
    43801 ccatttatca tcagaaagtt aattgaaaga ggtatcgtta aaactgtaaa atctgcaaga
    43861 aaacttgttg ataagaagga agctgttgtt tgggatatac ttgaaaatat attgaaggga
    43921 cacccggtaa tgctgaaccg tgccccaacg ctgcaccgtt tgtcaatcca ggctttccag
    43981 ccaaaattaa ttgaaggtaa agcaatacag ttgcatccgc tggtaacaac cgcattcaac
    44041 gccgactttg atggtgacca aatggcggtg catgtgccgt tgagccatgc tgctgtgctg
    44101 gaggctcaat tattgatgct ggcttcgcac aatattctta atccacagaa tggtacacca
    44161 atcacccttc cttctcagga catggtattg ggtctgtatt atatcacaaa gggtaagaaa
    44221 acaacagcaa ctgaaattgt tcgtggagaa gggaaagtat tttattcgaa tgaagaggtg
    44281 atcattgcct ataacgaagg ggttgttgac cttcatgcct ggatcaaagt aaaagcaaac
    44341 atccgcaacg ctgatggatt gctggaacat aaattaatag aaactacagt tggtcgtgtg
    44401 ttgttcaatc agcatgtacc tgctgaggta ggttttgtaa atgccctgtt gacaaaaaag
    44461 aatcttcgtg aaatcattgg tgatattata aagattacca acgttccaaa aacagcgaag
    44521 ttccttgatg atattaaaac actcggattc cgtacagcct tccagggtgg tttgtcattt
    44581 agtattaatg acctgatcat acccgatacc aagcaggaat tgctggagag tgcaaaaggt
    44641 gaagtagatg aagtgtggga tagctataat atgggtctta ttactaacaa cgaacgttat
    44701 aatcagatag ttgatatctg gagccgtgtc gacacaagaa ttacggagac attgatccgt
    44761 gaattagcga atgacaggca aggattcaac tctgtgtaca tgatgcttga ttctggtgca
    44821 aggggttcta aacagcagat caaacagctg gctggtatca ggggattgat ggccaaacca
    44881 agaaaatccg gttctacagg aagtgagatt attgaaaatc cgattctctc caactttaaa
    44941 ggtggattga acgtattgga ttactttatc tctacccacg gtgcccgtaa aggtctcgca
    45001 gatacggccc tgaaaactgc agatgccggt tacctgacac gtcgtttggt tgacgtttca
    45061 caggatgttg ttatcactga agaagattgc ggaacgttac gtggaatcgc tacttctgca
    45121 ttaaaagata atgaggatat tatagaaccg ttgagtgaca ggatcgccgg tcgtacatca
    45181 ttacatgatg tgtacgatcc aataagcgac cagttaattg tttctgctac tgaggaaatc
    45241 actattgata ttgctaaaaa aattgaagaa gcaggtatcg aaacagttga gatccgttca
    45301 gtacttacct gtgaaagtaa gagaggtgtt tgtgtgaagt gttatggtaa gaaccttgcc
    45361 tccggtaata ttgcacagaa aggtgatgca gttggtatta ttgcagctca atcaattggt
    45421 gagcccggaa ctcagttgac actccgtaca ttccacgtag gtggtgtggc cggttctgct
    45481 tctgtagaat caactttggc tgccaagttt gacggaacta ttcagtttga tggtcttcgt
    45541 actgttgtta ttgagaataa tgagggtgaa aaaacccagg tggttatcgg acgtacgggt
    45601 gaagttcgta ttatcgatac aaagaatgac cgcttgctta taaccaataa cattccttac
    45661 ggggctacat taaatgtaaa agatggccag aaggtaaata aaggtgatgt tgtttgtacg
    45721 tgggatccgt tcaataacgt aatcatggct gaaattaacg gaaccgtgaa atttgagaat
    45781 gttattgatg gtgttacata ccgggaggaa gcggatgaac aaacaggaca ccgtgagaaa
    45841 gtggtaattg agacaagaga taaaaccaag ataccatcaa tcctggtaga aggtaaagac
    45901 aagaaatcat ataacctgcc aaccggaagt catatcatta tggaagaagg agatgatgtg
    45961 aaggcgggtc aggtgatagt taagattcct cgtatcctcg gtaagttgcg ggatattacc
    46021 ggaggtctgc cccgtgttac tgaattattc gaggcaagga acccaagcaa ccctgctgta
    46081 gtttctgaaa ttgatggtgt ggtaatgatg ggtgctatca aacgtggtaa ccgtgaaatt
    46141 atcatcgaag caaaagacgg tgtgcagaag aaatatcttg ttccgttgac aagacaaatt
    46201 cttgcccagg atggggactt tgtaaaagca ggtgcttcac tgagtgacgg acaaattgcg
    46261 ccgttcgata ttctttcaat aaaaggtcca tttgctgtac aggagtatgt ggtgaatgaa
    46321 attcaggagg tttatcgttt acagggtgtg aagattaatg ataagcacat tgaagttatt
    46381 gttcgccaga tgatgcgtaa ggtgaatatt gttgatccgg gagatacaag attcctggag
    46441 gatgatctga ctgataagtt tgaatttgtg gaagagaatg actacatcta cgataagaaa
    46501 gttgtaactg aagtgggaga tagtacgaaa ctccgtgcag gtcagattgt tagtctccgt
    46561 gaagtaaggg aggaaaactc cattcttcgc cgtaacgaca aaaaactggt tgagtatcgt
    46621 gatgctaaac cagctacttc atctcctact ttgctgggta ttactaaagc atcgttgggt
    46681 gtgccaagct ggatatctgc tgcatccttc caggaaacaa cgaaagtgtt gtcttctgca
    46741 gcgatcaacg gtaaaaccga tgatatgctc ggattgaaag agaatgtaat taccggtcac
    46801 ccgatacctg caggtaccgg cctgagggaa tttgagaata tgattgtagg cagtaaagag
    46861 gagtatgagt tattacagac tacacgtgag gcgatggctt ttgatgaaga agaataattg
    46921 ataacagttt ctgactgaaa tgtatgtaat acaggagtaa gattttctta ctcctttttt
    46981 aatcccggac tggcctgtta agggtttcat aaagtaaact gaatccttat atttgcccgc
    47041 ctattcgtag ttattttgta aaaatgaatt tgatgaaaaa tggtttgttt atatggaatg
    47101 tggtgttgac cctggcatct ggttatcttc tcgtttcaca cttttctggt aagaaaatta
    47161 ccggcactgt aggaaaagat aatggcaagg cgacctcttc ctcttcgaac agctttcgta
    47221 ttgcctattt tgagatggac tccctggcag caaactttga tatggttaag gacctgaaag
    47281 ctgaaatgat caaacgggaa gaagccataa atgctgagat ggaccggctc tcaaaaaact
    47341 tacagcaaaa attcaattct tatcaacagc aggccacatc aggaagtatg acggaagagc
    47401 aatcagaagc agcaagcagg gatatgaaaa acctggatga tcagatgaaa agccgcaaac
    47461 aagccctgga ttccgattat agtgattttg ttatgcgaag gcaaaatgag attaaatcca
    47521 aaatagaaac tttcctgaaa gagtataaca ggacaaaaga ttattcttat attgtttctt
    47581 atgaacaggg attgttttat tataaggaca ctgcttataa tattacagct gatgtggtta
    47641 aggggctaaa tgaactgtac aagacgaaaa agaactaata aaattatatt ttaatgccct
    47701 gcttaccggg gcatttttat tttataccat ttctgtacaa aagaaatatt gccgtatttt
    47761 gagttatgga agattttaag aaactcttta cttcttttcc tgctgttaaa gttggagtga
    47821 ttggtgatgt gatgctggat acttatatgt ggggaaaggt tgaacgcata tcgccggaag
    47881 cgccggtacc cgtcgtcacg ttacagaaaa aggaatacag aattggcgga gccggaaatg
    47941 ttgccctgaa ttgccgttcc ttggatgcac aggtttccat tttatcggta acaggagatg
    48001 attcagactc cgtaatatta aagcaactcc tggaggataa taaaattgat acttcctatt
    48061 tagttccaag tgcccgcagg atcactacca acaagacccg tattatcagc cgaaaccagc
    48121 aaatgatgag gcttgatgct gaagtgacaa atgacctgga tgttattgat gaaaaattat
    48181 tgctggaaaa ggtggaggct tatattaaca ctgagcagcc aactgtcatt atatttgagg
    48241 attacaataa aggagtcctt tcggaatata ctattaaaca gattattcag tggtgtaaac
    48301 agtcaggaat tattacagca gtagatccaa agcggaaaaa tttcttctct tatactggcg
    48361 ttgatatttt taaacccaac cttaaggaag taaaagaggg attaaatcta cttgttgaca
    48421 atatcaatga gtcagtatta aaaaatattc atactgaact atgccgggtg ttgaatcacc
    48481 gtatttcttt tatcaccctc tccgaaaagg gagttttcta ccagcaggat gaaatagggc
    48541 atatcatctc atctcatatc cgtaatatcg ctgatgtgtc aggggcaggt gacacagtga
    48601 ttgcagtggc ttccttggtt tatgctgcta ccaaaaatgt acaattgatg gccgaagtag
    48661 caaatattgc cggcggtcta gtttgtgaag aggttggaac tgtcgcaatt aataaagaaa
    48721 aactactgaa agaatgtgag ctacttcttt gctgagcttt tttttaagtc attcatggct
    48781 ttacccatca atttgtcata atcaattaat aattcaaatg caatttttgc cagcgcaaaa
    48841 acgagtataa atatcttgcc taaaccaaga ctaagaaaca tactgccaag gatgacggta
    48901 aactgctgaa taaagattct accatacggc tgaaacatta tggccattat tggcattgtt
    48961 ttgtaatcgc cattcagcaa aaatgggatg aaacttttgg caatatagct gatgataaaa
    49021 gcacctagca tccaggcaat atcttcatta atataggtgt accatttaaa gaagaaatgc
    49081 atcataccac tgtttggtgg tgtaatttgt gcggcctggg aaaagatggt tgtttggacc
    49141 atggcaaata ttccaaaatg tataataaag aagaaaataa agaagaaacc gcttttcagg
    49201 gttgttttcc cattggcatg ccattcatct ttgccttttg caatagtagc gatcagcatt
    49261 ttaaggatag tgaggattcc tacaatcatc gtttccattg catacacgat aaatgcttct
    49321 gtggggctcc atcccaaaaa ccatacaccg aaaaccggaa tcaggttggc aattattaaa
    49381 agcaggtctg agccagatat atttctttta aacatcaaat gaaaattttt cgattacaaa
    49441 atacctaatt tcgttcgttt attaataccc atttacccgt atgttgaaat ttacaatagt
    49501 tattcatggt ggcgcaggta ccattctgcg ttcagcaatg acaccagaac tggaaaaagc
    49561 ctataaagaa gggttggaac aggcgttgca tgcaggatat acgttgctgg aaaagaatgg
    49621 tacttcattg gatgcggtaa aagcggctgt gatgtcactg gaagattgta tccttttcaa
    49681 tgccggaagg ggatctgttt ttgcaaaaga tggcagccag gaaatggacg catccataat
    49741 ggagggtaaa acattaatgg cgggtgcagt atcagcagtc cgcagtatta aaaacccggt
    49801 agacctggct tatgtggtga tgacaaaaag ccaacatgta atgctgaatg gaaaaggtgc
    49861 ttatgatttt gcgttgcggc atggcataaa aacggaacca gatagttact tcttttcaca
    49921 attccgctat gatcagtgga aagagttaca ggggactgat gaagcggcac ttgatcataa
    49981 tgtaccaagc aaggagaaaa aattcggaac ggttggggct gtggccctgg ataaacatgg
    50041 gaatattgca gctgccacaa gtacaggagg tatgacgaat aaacaatatg gcagggttgg
    50101 tgatagtcct ataatagggg ctggcactta tgccaataat aaaacttgtg cgatcagttg
    50161 caccgggcat ggtgagcctt ttatccggtc agtaacagcc tacgatgtaa gctgccttat
    50221 ggaatataaa ggactgtcat taaatgatgc catgcattta gtggtacatg ataagttgtt
    50281 gaaaattgat ggtgaaggtg gaatgatagg agtggatgca aaagggaatg cagcaatggt
    50341 ctttaatagt gaaggaatgt acaggggaat gaaatgcagc gatggaagaa atgagattgc
    50401 tgtatacaaa taattattag ttgtttccat ttacatttgt cctatgcaga aatatgctat
    50461 aatagttgcc ggaggttcag gaacaagaat gggaagctct ctgcccaagc agtttatact
    50521 gctaaaagat aagccagtac tatattatac attgaaggca ttcctggaag catatgatga
    50581 tttgcaaatc atccttgtat taccagttga atatacaaat atgggccagg aaataattga
    50641 cgcttatttc gataaagaaa ggataagaat cactgccggg ggtgataccc gttttcaatc
    50701 tgttaagaat gggttagcta tggtagaaca ggaatcggtc attttcgtac atgacggagt
    50761 aaggtgcctt ttatctaaag agttgatcca acaatgttat caacaggccc tggaaacagg
    50821 aacagccata cctgcaattg tttcaaaaga cagtatccgg ttggttaatg agaaagggaa
    50881 tgaagcgttt gacagaaata aagtgatgtt aatacagacc ccacagactt ttcatagtaa
    50941 aatattgtta ccagctttcc agattgatta taaagataaa ttcacggatg aagcaacagt
    51001 ggtagaagcc tacggcatga aagtatcatt gattgatggt gaagagaata atattaagat
    51061 aacccggcca attgatttat tgtttgccga aaaaattata gaagagagag ggcgacagca
    51121 agaagctgat ttataatgtc aattctttaa ccattttaaa taaaatggca gtctgttaat
    51181 ctttaatgca ggataaacct cttgtttcgc accaaagctg gcatagaaga aagcaagatt
    51241 gcggatatca gatccttcga agtccagtga catatattca ccggcatgat cttttataaa
    51301 ggcatcaatc aatgcatgcg aagcgccaat tgttttcccg tttggatgat tacctacgag
    51361 tatataatag gcccgtttat gggagaaaaa aaatacacag gaggccagta attcttcatt
    51421 taatgaaatc ccgtatgttg ttgccatttt tttttcatga agatatgtat acagtttacg
    51481 gaagcggtca acattatatg ccgactcttt gccataacct tccatttgct gtaaagccag
    51541 ttcaattact tttcccgcat caaaatctct ttcgacccgg cagcctaact ggttagcttt
    51601 tttaatgttt ctttttatat tttcccggta gcccttgtaa agctcagcgt aggaattatt
    51661 taaatcaaga acataattac ttctgtgaat ggagaatcct gtgggcactc ctgatatgtt
    51721 cccactattt aatgcgatgt caatgaaccg gaatgaagac ggtatagatt gaataaaagt
    51781 accaacttgt tcttcagtaa tcttattgcc aaatactcct aattgtgcag taagaaacgg
    51841 ttggtacaga taattgatgc catacttcct gttccatgtc aggggcatca ctgctgcata
    51901 gtcgtttagt accaatgcat cccagttttt tgacatctgg tcaagataga aggaatatgc
    51961 ataaattaaa ccattatcag ccttgccaat acaatcatcc catttggcct tgtcaatctg
    52021 gtaatgggtc aggtattgta ttttgagcgg ggcactcatc agaaatcaag attacggtca
    52081 aatttccgga agctgaaatt ggagatatcg atggaaaaag agatcttttt ccaaaacctt
    52141 ccttttttct ctatcgttga ctgaatatag tctttgtaaa tattactata gatcaatggg
    52201 acataaatgt ttaccgtttc tttcaataag ggtatatgga tacctgcgtc aaaaacaaaa
    52261 cgatctgttt cagccccttg cttccaggtt tcagcataag tgccaatatc aaaaaacaaa
    52321 ttcagtggta ttttgaccgg taacaaactt aatggattga tagaagaagg gatggtggta
    52381 ctgaaattag cggcaatcag ccaatcatct gttttgccga ctttttcagc cagcaggtca
    52441 gttcttactt taaaagctcc gtctctttcc atgatctgct gactgggtgg cccttcaaat
    52501 ttgtttctgc cgataaaata atcactgtat gtgtaatcct catagccatt ggcgccagtc
    52561 atattcaaat gataccggtc agtagcaaat tgttttgtaa ttgttttgga ggtgctataa
    52621 aagaatttgc cggcaaacag gcgaacattc agccctcctt ctttggcata attaaagaaa
    52681 tattttccgg cgaaggcagt acgtataaag tctttaccct gttcaatttt tagttcaccg
    52741 cgataaggat aaagagccct gttgttttca atcacaaatt gaacctgatt tactgttctg
    52801 ttttcactgg tagttttgta tttattgaga atacttgtat caggccccga aataaccgtg
    52861 tcacgataga aacgaagccc atcttcattt atcaggaatg atttaaactg tacataacgg
    52921 ttgagactgc ttctgggatc ttttttgttg agtgttaatc ttattcccgg tactatttta
    52981 ctgaagccaa gaaatgtttt attattatca atatctgtga aacggtcaac ggtaaaagta
    53041 gagccactaa ttcctatatc aattttcctg aaaactttat tgggataaaa gctatagttg
    53101 ataaacccga tgccattaaa attctttgac gcaaaaccat acatcggtac tataaggaat
    53161 tgaaaacggc taggaggtag ccgaagatta gttagcagag cacctgccat caatttgtta
    53221 taactgttcg aacctaaaat gggaaggagc gtaatggcag attttttatt actattaact
    53281 cctgaaactg ctccgattgg cccctggaat ccgatcccct ttttctccga tcccggcaga
    53341 ttaccccttt gccgcagcaa tgcaaatgtt gtatccaggt tcttgccact aatatcctcc
    53401 agtatttttt taaaatcttc tggttgagga tgtttgaatt gccattgacg gtaatactcc
    53461 tgcatggctt tattgaagac tgccgtcccc aattgttttt ccagtaaggt catccatttc
    53521 gacgctttgt aataagcaat caatccgtaa ttgggttcat taaaatcttc tgatgatgtt
    53581 ccaatgggct gatctttttt ctcaacaata aacgtttcca gtaagctttg ttctgtgcgt
    53641 tgtccaaaat gatcattttg ccgttgctgg tatttacttt catagaatga attcattcct
    53701 tcatccatcc atgcatattt tctttcattg gtgccaagaa taccgtagaa ccaattatgc
    53761 ccgatctcat gtgcaattgt aaaatttaag gagcggctgc ttttttccgg cgacaataca
    53821 gtaatcgttg gatattccat acctccgcca aaacttgcag gaccctgcac cacactcact
    53881 acattgtaag ggtactcacc aaccagtgat gaataatgct gaattgcatc ttttgcatac
    53941 agcaaactgc ttttccatac ttccttttgc ggagtagtgt aataagtaaa aacatcgatt
    54001 actttaccgg aagccagctt acaggtatca tagtttacaa taaatcgctt gtcggcaaac
    54061 caggcaaagt catgaatatt attttgtttg taggttaata ttttaatttc cttttctgtt
    54121 tcagggaata gttgataact cgttttaacc tggccacctg tagtttttgt tttttgttta
    54181 acaggctccc aggtaaaagc agctctcgat ttcagccatt ctgtttcagc tgcatgttgc
    54241 agctccccgg ttgctgcaac gacataattt ttcggaacag tgatgtttac ttcaaaattc
    54301 ccaaactcgc tgtaaaattc tccctggtca agatatggca tcgggtgcca gccattttta
    54361 tcatatactg ccggtttggg ataccactgt gtagcctggt agctttggcc atcatgtcct
    54421 cctctcgaaa aattgtaagg aagttttacg tgaaatggag tggtgataat tgttttttgt
    54481 ccaggtgaga gaggagctgg taaaagcact tttacaatat caatatgctg ggggtgatct
    54541 tctacctgtg ctgtaatatt gtttacttta aaatcaagtt tattgatata gcctttatct
    54601 tctttatctg agaaataaaa cctggtactt ccattttcca gcaaatggtc actgaatgct
    54661 gtcttatcat tcttataggc atttggccag agatgaaacc agatgtattt aattgtgtcc
    54721 ggcgaattat tgatgtattc aattttttca aagccgttca acgtatgccc tttatcatcc
    54781 agggaaacat cgatgctata attaacttgt tgttgccagt attgggaaag agcaacctgc
    54841 cgtgagctag taagagtgaa caggaatatg agcaattttc tcaaaggatg gagttttctc
    54901 aaaaatagtt taaaaacccg aactgcagaa ctgttagaga attgaatctg attgatacaa
    54961 gctgattatt taccttttcc ttttaagata attcgtattg tatggatcat gattttaaaa
    55021 tccagtacca gtgaaatatt ttctatgtaa agaaggtcaa atttacttct ttcgatcatc
    55081 tcgtctgtat tttcagcata gccaaattgt atcatacccc aactggtcaa tccgggtttt
    55141 actttcagca ggtatttata atagggattg cgggcaataa tctgatcaat ataaaaacgg
    55201 cgttccggtc ttggccctac caggctcatt tcacccaaca gtatattcca gagttgtggt
    55261 aactcatcca gccgccattt acgcattacc ttcccccatt tggtaatgcg ggaatcattt
    55321 tcagaagaaa gcgccggtcc gtctttttct gcctcattaa tcatggaacg aaatttatac
    55381 attgtaaaag gcttgccctt gtaaccaatc ctttcctggg aatagaagac cggtcctttt
    55441 gaagaaaatt ttacccttaa tgcaatgtat accatgaaag gtgaaagtaa tacaagccca
    55501 aagagagctg aagtgacatc aataagacgt ttgaagttct gttgccattg cggcatcaac
    55561 cccgtttgta aatcaattaa tgcggcaccc atcacattgc ttgtctttac ggaaccggaa
    55621 agaatatcta atgtgtcggg ttggattttt acttctacat ctttttcact aagcctgttt
    55681 atgatatttt ccaatagtgg ttgttcagtt ttttccaatg ccagcacaac caaccggata
    55741 ttagtttgat ctattatttt ttctaattca tgtacggtgc ccaatttagg cagtaattta
    55801 tgaatgccat ttcttccgtt attgtcaggt gttataaaac ctgcataacg ataaccgcca
    55861 tcatgcaggt tattttctgt ttctttgaaa atacgaattg cattttcctg gctacctacc
    55921 attaatgtgt tgaaaaatac ttttcccctg agcaattgtc ttttaatagc attcagtata
    55981 atccatctgc ctgaaaaagt aagtaaaaaa tgtatagaaa gtaaggtggc aaaagccagg
    56041 taaaaatagg cgtatccatt attcggatcg aaatcattta atacaaatac tataaataaa
    56101 acgataactc caatgagact gcaaacaaat gtcagtgtaa attctgctaa tcttgatttc
    56161 ttataaaggt agttataggt cccggcaatg gtatagagca ccagccatcc ggcaggcacg
    56221 attagtaaaa tatataacca tgatgtataa ctgatgggat aagcaccagc atcattcagc
    56281 atccagcttc tccagaaata aaagcaaagc catgtcaggg ctgccatcac aaaatcaaca
    56341 gctacatacc aggcaaggga tatttgtttg ccggattgca ttaatgcgag atattattat
    56401 taccaatttt ttcaagtatc tggtgggcta catccatggc catgagccca tccatttctg
    56461 atacaatggt tttagtattg ttaaggatgg catttttaaa ttcctccagt tccattttta
    56521 ttgcattcac ttcgggaaca accgggttag ccattgctat agtttttttg ccagttgggg
    56581 tttctatatc aaaagcaaaa acgttggtat cacctggccc tttaagtttt attatttcgg
    56641 ttttcttatt caggaagtca attccaatgt aagagtcttt ttgaaataac ctgatctttc
    56701 tcattttttt cattgaaatg cggctgctgg tgagatttgc cacacagcca ttatgaaatt
    56761 cgatccgtac gttggcaata tcaggggttt ctgtcagtac acctacacca ctggccgaaa
    56821 tactcttcac atcacttttt actatactta aaataatatc tatatcatgt atcatcaggt
    56881 ccagtatcac actcacttct gttcctcttg gattgaactg ggcgagacgg tgcacttcga
    56941 tgaacatggg atttaattcc atgtctttta tggcgagaaa agccggattg aatctttcta
    57001 catgtccaac ctggaattta atacctgatt cagcagccaa ttttaccagt tgccgggctt
    57061 cctccatagt attagccagt ggtttttcaa caaatacgtg tttccctttt ttgatggcct
    57121 tttcacacca ttcaaaatga tagttagtag gagcaacgac atcaatagca tcgcaggcat
    57181 ctattaacgc atctgcatct aagaaacggg gcagttgata tttttcggaa acttcttttg
    57241 ctgtttcatc atgggggtca taaaagccaa ccagttccgc accggcaatc tctttccagt
    57301 tattgagatg aaattttcct aaatgaccta caccaaaaac accaatcttg agcataacaa
    57361 tttattaatc agcaaagata aagcttgact ggtgcagaaa gctgatagtt atgaacaaat
    57421 tgctttaatt caaaacttcg gactgatcaa tgtaagatgg atgataaatg aaataacaag
    57481 taccgttttt gattgtgttt atgatgggtg tggatgtatg taccggaact gccgggcagc
    57541 cctggctacg gccaatgaaa ccctgcgcat ttgcaatggc atcagaaaca tattcagccc
    57601 catgcactac aatccccctc tgataagcct tgtcattaat accgctttct atgccctcga
    57661 gtttcagtga aagaccatgt ttgccctggt aagtttccct ggtaacataa aaaccaggac
    57721 tgctcttata agaagagctt tggttggaaa aggaagtggc cagttcacgg cctgtatttc
    57781 ttccgtgtgc gaccaaggta ttgaataaga ctttgtagtt tttcaagtca ataacaaata
    57841 atcttttctt attgcttggt tggctgaagt caacaatgga aatgattgaa ttattaagga
    57901 gttttccttc ttcaatgagt ttgtcaaatc ccctttttgc ataatcaaaa gcatctttgc
    57961 taagacccaa aagacttaaa tgcaggctgt cataaagcga ccttgtttcg ggagcgaaat
    58021 acatagagtc tgaagtattt cttattgcag aatcaccggg atgaaaagac atccggctgc
    58081 tacccgaagc agattttgca aatgcaaaag gtaaatgcag gagagcaatt acaagcgaag
    58141 agagataaag ataaaatcct ttgaacctgt ctttcatagc tactattttt taaccattaa
    58201 ccgttaacct ttagacgccg tagcgatttt attattgcaa gacaagcctt aaccctgatc
    58261 aataaaaaat gcccgtgatc tctcaccggg cagtttttag ttttgatttt tgaaagttta
    58321 acaacaagct aacctttcta tttgcggacc ggacgggact cgaacccgcg acctccgccg
    58381 tgacagggcg gcattctaac caactgaact accgatcctc cttcctcgct atgctcctca
    58441 ggatgacaat ttcatccaga ttttacactg cgtttttagg gacggcaaag ataggcctgc
    58501 aatctttttc ggccaaaagt ttttctgcta attatccacc tgtaattcaa cattttggca
    58561 tcatgcccat attatcattt aatccttaat ttagccactt cataaatagc ctatgccttc
    58621 agacagaaac cgacagttta ttgactttga aaagccaatt aaggaccttt tagaagaaat
    58681 agaagatctg aaacaaaaag ctgaaaaaag taaggtggat tacagtgaag taattcataa
    58741 gcttgaaaac agtgttcttg agaaaagaaa ggagatcact caaagcctga ccagctggca
    58801 acgggtacaa ttaagccgtc atcccgacag gccttatacg ctgaagtata ttactaaaat
    58861 gaccagcaat tttgtagagt tgtatgggga tcgtaacgtg aaggatgata aagcaatggt
    58921 cggtggtttt gcttcattgg acggggaaac agtaatgata attggtcagc aaaaagggat
    58981 aaatactaaa atgcggcaac tccggaattt tggtatggcc aatcctgaag gatacagaaa
    59041 agccttgcgg ctgatgaggc tggctgaaaa atttaataag ccggttatta ccctgataga
    59101 cactcccggt gcttatcctg gtctggaagc ggaagagaga ggacagggtg aagctattgc
    59161 ccggaatatt tatgaaatga tacgacttaa agtaccagtc atttgtgtaa taattgggga
    59221 aggggccagt ggaggggcgc tgggtattgg tgcaggcgat aaagttttta tgatggagaa
    59281 tacatggtac acggttatca gtcctgaaag ttgttcttca attttatggc gcagttggga
    59341 taaaaaagaa gtggccgcag aacagttacg gttaacagcc gataaaatgc tgggcttcgg
    59401 tttgatagat ggtattatcc cggagcctcc tggtggtgca cactgggact atgatgaggc
    59461 ggctcaaatc ctgaaaaatt acctggtacc ggtattgaaa gaattgaaac agatcccagt
    59521 tgaacaaagg gtgaataacc gcattgagaa gtttggcaag atgggattct gggaagaagt
    59581 ataaatagcc ggtgtcttta gtcataaaac gggatcaggt atattgtgcc agcttaggga
    59641 ttgctataaa cttttttttg tatcactcac ttcatcgtta ggattgctgt tgaagatttt
    59701 gggtatcaat atttattcat tcatcacaat taatttttta ttcattaaat gtcactcagg
    59761 aaaaaattta ccggtacagg cattgctatt gtaactccct ttcatgcaga cggaacaatt
    59821 gattgggata gctttaaaaa actcattcag ttctggatag aaggaaaaac tgagtacctg
    59881 gtggtattag gcactactgg cgaaagtgcc acagtgcatg gtgaagaaaa gcaacaggta
    59941 ttttcttttg tgaagaatga agtggccgga cgtttacctc ttgtggctgg cattggcgga
    60001 aatgataccc atgaagtaat caaaggattg aaagaatttg atctttcagg atatgatgca
    60061 atactttcag tgagtcctta ctataataaa cccaaccagg aaggaatttt tcagcattat
    60121 aaagcattgg atgcggcaac accattaccc atcattatgt ataatgtgcc tggccgtacc
    60181 gggcaaagtg ttacagccga tacacagatc cgtatagccc gtgaatgcaa aaatgtattt
    60241 gcgaccaaag aagccagcgg aaattttgat ctgatcaacc agatcatcag aaataagcca
    60301 gatgatttca tggtcatcag cggggatgat cccattacat tgcatatgat agctgcaggt
    60361 gccgaaggcc tgatctctgt tgttgctaat gcttatccaa gagagtattc agatatggtt
    60421 cgtttctgcc tggcaggaaa atttgcagaa gctcaaaagt tgcattataa atatattgat
    60481 attatcactt ctatgtttgc cgaaggaagc cccagtggtg ttaaagctta tttatcggaa
    60541 atgggactat gtcagaatac atttcggttg ccggtatgga aggttagcga aaaacatcat
    60601 actaagataa aggagctgat gaaggatgta gggtaaatat ttttactgta tgttattaag
    60661 aatatgcatt ttaaatcttt tattgctgac cggtttttat tcatttgctc aaaatgaaac
    60721 aaaaccagca ataaaaagca gtgcctccgg caatgtcatc attatagata cagcttttgg
    60781 aattccccaa cttcaacgct caagaaaaat atggatttat ttgcccgcag gttattccac
    60841 ctctaagaaa aaatatcctg tattgtacat gcatgatggc cagaatgttt ttgacagcgc
    60901 taccgcttat gcaggagaat ggggagtaga tgaattttta gattctgtga aactgaagga
    60961 atcaattgta gtggctatcg acaatggttt aaataagcga atgaatgaat ataacccgta
    61021 tgattcggaa aggtttggta agggtgaggg aggtttttat gttgacttcc ttgtcaaaac
    61081 attaaagcca tatattgaaa aaaaatatcg gacaaaaaaa tgccgtaaga gcaggtttat
    61141 tgcaggcagt tcaatggggg gattgatctc tttatatgct gttctgaaat accccagggt
    61201 atttggagga gcaggaattt tctcgcctgc attctgggtt gccccgaaga tttatgatga
    61261 tatacaatca aggggcaaga aagtgaaagg taaactctat ttctatgccg gtaaacaaga
    61321 aggtgaaacc atggtgccct atatgttgaa agcatttgag gcacttactt cggtttccag
    61381 atcgaaaata tcaacagtga ttcgggatga gggaaagcac aatgaggcaa catggagaaa
    61441 ggagtttccg ttgttttatg agtggttact taaatagact ttaaataaaa tgaagtcatc
    61501 tgcctctgta tgaaatcaga cggtatatgc taccccctct aaagccagat cagtattcgg
    61561 gaatacttct ctcgcttctg cctcaaattc ttccagtgta tcatatttac tgctaaagtg
    61621 accgatcaat aatttcctga caccggcttt tttggcaatc aatgcagctt gtttggttgt
    61681 ggaatgaaaa cgcagttctg ccctttcccg taggttatca agataggtgg tttcatgata
    61741 gatcatgtca aaaccttgca catgcggaat gagtgattcg tcatattttg tatcggcaca
    61801 aaaagcatat tttctgccag gatcgccggg agttgttacc gtttcatttt ttatcagtgt
    61861 tccgtctttt ctggtataat cttccccatt ggtcaaccgc tcatagaatg aagcggggat
    61921 ctcgtactct tttgctttat cggggttaag
//
LOCUS       DAORTI010000007        58232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_275_length_58232_cov_10.082094, whole genome shotgun sequence.
ACCESSION   DAORTI010000007 DAORTI010000000
VERSION     DAORTI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 58232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58232
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_58232_cov_10.082094"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLO70_02000"
     CDS             complement(<1..506)
                     /locus_tag="PLO70_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73250.1"
                     /translation="MPRKNLLFLQEAIVVALINQPSRTASFEEIANFIEQRGLFSVRK
                     GNISLAKQLMLRSTLSKGKYKHLFDEIGAGYIRLRDSYADFGIMLSNSLDNILDSHCK
                     LYKSNPETIRVKDLKMNGFREVSLSASDIICIATREKSGEKKYIYVLERDIIGQSAIG
                     VYTINKNL"
     gene            complement(508..972)
                     /locus_tag="PLO70_02005"
     CDS             complement(508..972)
                     /locus_tag="PLO70_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73251.1"
                     /translation="MNPERKVDAEKIHLLNIKTLSGNIDGATDADINAVVGHQFNFEL
                     RTGLNPDDNIIGLQLVVGIEARDKTDKPLNVKGSYTHEIVFKVENLIDFLEKVETDDK
                     PKYNIDGGLGSTLVSIAYSTIRGIIFTRTQGTSLGAVILPVIDPKKLMGIQV"
     gene            complement(969..1412)
                     /locus_tag="PLO70_02010"
     CDS             complement(969..1412)
                     /locus_tag="PLO70_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ73252.1"
                     /translation="MKKKVLKAEAIPEFEESISSMSDDSKIFVDKSLEIADFIYWIME
                     QKGMKQKDLADKMGKSEAEISKFLAGMHNYTLRSIAKIESALGATVICTPRTVRVSFP
                     EESVHSSEYHIVEKRQEGHVPVIHYDTKVISIYSKEQNHPEPIAI"
     gene            complement(1424..1816)
                     /locus_tag="PLO70_02015"
     CDS             complement(1424..1816)
                     /locus_tag="PLO70_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73253.1"
                     /translation="MQHNEKDKKQVAEINRFIENIGKKHGAQDRFFKREGFAERLPPA
                     TYRFLDSDGETDFGLRLYCVRISEEVVILLNGDRKTAQSIKDCLNCKPHFDFANKISD
                     AIYEAKTKDRIEIDGFDILMDDDFVLNI"
     gene            2617..3081
                     /locus_tag="PLO70_02020"
     CDS             2617..3081
                     /locus_tag="PLO70_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73254.1"
                     /translation="MNKKIIAAVCAGFFFSMAAVCQTVLFINAGSRASYGQQQELLVD
                     ANGNCRYRLGTVNGPVKDSSSFALLPGQLDSLFRQAVATGFFSLQHNYDGGAADGAGI
                     YISMNHAGQQHYVQLKNTDVPAINQFITLLNTMLAPRQIRINYGQFVPPRPR"
     gene            3078..4301
                     /locus_tag="PLO70_02025"
     CDS             3078..4301
                     /locus_tag="PLO70_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73255.1"
                     /translation="MNRFYYITSIAVFFMLLQSCTGTRRFGTTIERDENNPCQVNMII
                     QVAIEGTDDDVTMVKSALEDCYGKECFIPCPGDSLKGCKTKITVVVKKYSSLKEDETA
                     GFHYVQMIDDDGLPSNAYLGTPNNGASSGTWRRNQPPGVYCHEVLHFCGLPDKYCSRL
                     YDPVTDSARTELVCDPPPDPNGYCCAPSASHTRCSTPCAGHEHDLMANIYTPLSCDNI
                     MDVLKGAGLNNCPPECCASDKTFTRPPDEVYIIPGYYHFGDKNTKFGSIGGSVGYTKQ
                     IGTSLGVTIEGGYYLHSDKDNSYKQTSGLMHITGGITYRVTDPPTTEKGISISTHALF
                     GVSRYTQKTSFGGNTLKDDATSLHFNIGAAVDLQLNRSWAVRLLQADYAPTFFYEATQ
                     HNYRISAGLVHRLGR"
     gene            complement(4419..4760)
                     /locus_tag="PLO70_02030"
     CDS             complement(4419..4760)
                     /locus_tag="PLO70_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73256.1"
                     /translation="MKKIKHIKDIQEEKMRLRIQQLEQEKNLQQNWNETKLELRPGTL
                     LRNQMAASSTGKEGENTLFTGLVSLGAGYLTRRFTGLAGQKIENTVQMGIEKLANRMQ
                     AVLKKKRKRKK"
     gene            complement(4747..5112)
                     /locus_tag="PLO70_02035"
     CDS             complement(4747..5112)
                     /locus_tag="PLO70_02035"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQZ73257.1"
                     /translation="MSEEFNKVESMIEQLKAYANTRVSQVKLSMAEKVSKLAAEMIAM
                     LMAALVFFLFLVILSIAAAIFIGQWLENMWLGFVIVAGVILVVGLILWLSRDRLLRIP
                     IMNKLIEALFEPEEDDEKN"
     gene            complement(5134..5358)
                     /locus_tag="PLO70_02040"
     CDS             complement(5134..5358)
                     /locus_tag="PLO70_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQZ73258.1"
                     /translation="MKNTSKILIALGAGLAIGGLMGVLFAPDKGENTRHKIAENGKKL
                     KDQFTRKVKLGKNKMEEQMARINGELDEVI"
     gene            5670..6152
                     /locus_tag="PLO70_02045"
     CDS             5670..6152
                     /locus_tag="PLO70_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ73259.1"
                     /translation="MKSKFLIVALSGLSALFFSACTTPAEEAKPIDMDKLKVEIQALE
                     DAYAAAEKAKDADGVVAYYSQDAVSYSRNEAPLSGAAAIKEKIAKRLASDTTGNTSVY
                     KVVDLFAEGNSATEIGSWTTINPAGAEVEKGYYMSFFQKRDGKYVCVRDMNVTTTPAK
                     "
     gene            complement(6459..7835)
                     /locus_tag="PLO70_02050"
     CDS             complement(6459..7835)
                     /locus_tag="PLO70_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQZ73260.1"
                     /translation="MDQHNSSGTPQSLVIPMQGTGGAGEQSTVRPVTRADRIKSIDTI
                     RGVALLGILMMNIPGFGINWDFWNSILTGPRAGKDYYTFAIVFVFFEGTMRGLFSMLF
                     GAGMVLFMQNKHELPGRPTVAEYYYRRLLWLVLFGVINAFVLLWPGDILFFYGLCGML
                     LFAFRKLSPKWLVVIALTCVAFGMVKKQWDWNETREKRESYTEAMAAKKEKKELTPEQ
                     QGAIKMWTEMEKSQKPDTAITNENLRKMQSGYGTIFTYFIPRNAGGEIWYMYNEVWDL
                     LVMMFLGMALYLWGFFSNRLRTSTYMMWLLIGYGIGVPIAWAIFDKGFMSFGRMGLYA
                     DSWSSSHMVFAEFRRILITIGHASLVMLIFRSGIIPWLMRALANVGQMAFTNYLMQSI
                     LCTLFFYGYGLGYYNKLKFHQLYYVVFAVWIFQLIFSSIWLRYFRFGPFEWLWRSLTY
                     WKKQPMKL"
     gene            complement(7861..9357)
                     /locus_tag="PLO70_02055"
     CDS             complement(7861..9357)
                     /locus_tag="PLO70_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQZ73261.1"
                     /translation="MSLHPSTPSGTAPDQVIANEPVNLSRLFSLDVLRGIALLGILVI
                     SIWEFGGFTSNEQTFFRQGTHGGNYKLLSIVSILFEGKMRALFALVFGAGIILFMQKK
                     EHPVPLAAPDAYIRRQIWLIIFGVFNAFVLLWPGDILFQYGVVGILLFAFWRMPARGL
                     LIAAILCTLIYCGKQYWNYADDKKGYKKFLAVTAVEKKFKQDSTARAKKDSFDRTKDT
                     VLLKDTLAKNKLMDSLARKNDTLTKKQAGEKGMWEGMIKDLKYDSSKTVAENKAIRAS
                     YGKVWKHLMQRSQNKESNWLYRTGVWDISSMMLLGMALLGFGFFNHRMASSRYVLIAV
                     LALAIGFTLAWFRIHFQSIKLADYARYIEKYPVPFNQFFPIERMLLATGYASLVMLLL
                     RSKFLNWLWQALAATGKMALTNYIMQTIICTFFFYGYGFGYFGRLQQWQLYFMVAEIA
                     LVQIVFSVFWLRYFTMGPLEWLWRCLIYRKWLPWKIKEPHAITDSPAL"
     gene            complement(9446..10099)
                     /locus_tag="PLO70_02060"
     CDS             complement(9446..10099)
                     /locus_tag="PLO70_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ73262.1"
                     /translation="MEINIAILMADLSGYTAMTEIHGAESAAAMVDRYLTLVNKSLVG
                     DSHLHERIGDQVVIISSQAEQLAYTATFLFEFAHEQEDFLPLHAALHYGPVVKKDNAY
                     FGSTINTAARIMSAADKGKILCSADFLDQLPQGHSFAVKKIGLHQFKNLLQQVDLFEL
                     DCCITNLSKTFVIDPVCHMLLKSPATSLQLKHDDTIHYFCSEKCRALYKKLYMADET"
     gene            10217..11056
                     /locus_tag="PLO70_02065"
     CDS             10217..11056
                     /locus_tag="PLO70_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ73263.1"
                     /translation="MSLYITSLNSGSNANCYYIGNNNEAVLIDAGLSCRETERRMKQL
                     ELSMERVKAIFISHEHADHITGLAGISKKYQLPVYITDSTLVRSNMPVEPQLVNSFKD
                     AKPITIGNLAITPFKKSHDAADPHSFMVAGHGVNVGVITDIGYACKRVIKYFSQCHAV
                     FLESNYCDDMLRNGNYPYHLQKRISSDEGHLSNAQALELFQQYQSADLRLLILSHLSK
                     NNNKPELVEAMFNQHAGSTQIVVASRYEASPVFCIEGIAVTTAAKPKRRMIIKDERQL
                     SLF"
     gene            complement(11082..11960)
                     /locus_tag="PLO70_02070"
     CDS             complement(11082..11960)
                     /locus_tag="PLO70_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73264.1"
                     /translation="MRSLFLFITTCFAFMQCQKEVNSAQVTVLPFEAVPVTTPVLPGT
                     IDEASGIADSKGNTGFIWVQQDGGNPPELFLLSYTGTVQKKIPVKGVTNRDWEDMAVG
                     NGPVAGTNYVYLAETGDNSASYTNYAIYRFAEPTLTTDTVFNTDKIVFQYPDGSHDAE
                     AMLLDNSTKDIYLISKRDSASRIYKLPYPQSTTTTMIATLVGEMPFTGVSAAAASPDG
                     KELLVKTYTNVYYWKRNAGETIGAALLRTPLALGYMLEPQGEAICFKNDNSAFLTLSE
                     KPFFATSVTLNLYRRK"
     gene            complement(12065..12721)
                     /gene="pdxH"
                     /locus_tag="PLO70_02075"
     CDS             complement(12065..12721)
                     /gene="pdxH"
                     /locus_tag="PLO70_02075"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQZ73265.1"
                     /translation="MKPDEIAQLRKDYTLHTLNEDEVDPSPLAQFQRWWNDAEKSQID
                     EMNAMTLATTGHDGMPEARTVLLKAFDEKGFVFFTNYNSAKSQQLDFNANCCLLFFWK
                     ELERQVRINGIAEKISVKESIDYFDSRPDGSKIGAWASPQSVVVAGKTWLKETFEYYA
                     ERFKHGKIPKPPHWGGYRVKPTKIEFWQGRPSRMHDRILYTPVADSAEGQWKTERLAP
                     "
     gene            complement(12760..13332)
                     /locus_tag="PLO70_02080"
     CDS             complement(12760..13332)
                     /locus_tag="PLO70_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQZ73266.1"
                     /translation="MNDFKKLEELIVAGFGGQGVLSLGMTLAYAGMVEGKEISWMPSY
                     GPEMRGGTANCITIISEKKISSPIISLFDSAIVLNQPSMDKFAPRVKPGGLLLYESGN
                     IFKPSTRTDIEIIGIPASTEALLMKNAKIMNMIILGAYLQLKPVVKIDSILEALKKVL
                     PEKYHHLLDINKQALEKGKALVVQQKEPVM"
     gene            complement(13451..14269)
                     /locus_tag="PLO70_02085"
     CDS             complement(13451..14269)
                     /locus_tag="PLO70_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQZ73267.1"
                     /translation="MPTEEHVLPDTALDVIAPACHDEEEYEMVYHKPSTMVNVAMHYC
                     PGCAHSLVHKLIMEVVEEMDIQEKTIGIAPVGCSVFAYEYMDIDMQEAAHGRACAVAT
                     GIKKLMPDKYVFTYQGDGDLAAIGIAETLHAINRGENILIVFMNNAVYGMTSGQMAPT
                     TLAGMHTTTSPDGRDVSYYGAPIKVSELMANLPGAFYVTRQAVNSANAVRRTKKALQN
                     AFKYQQLHKGTCLIEIIGNCPSNWKMTPVEASKFIDEEMVKTFPLGDIKVPKGV"
     gene            complement(14259..15326)
                     /locus_tag="PLO70_02090"
     CDS             complement(14259..15326)
                     /locus_tag="PLO70_02090"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQZ73268.1"
                     /translation="MTETKLMKGNEAMAEAAILAGCDAYFGYPITPQSEVLEYLAREM
                     PKHNRIVMQAESEVASINMVYGAAGAGFRAMTSSSSPGISLMQEGISYIAGAELPCLI
                     VNVNRAGPGLGTIQPGQGDYFQSVKGGGHGDYKMIVLAPASVQEMADFVYLGFDLADK
                     YRNPVLMLSDGAIGQMMEKVQFKPYTRPAVDKSWATTGKPKTRNRNYITSLFIQPERM
                     EQHNLKLEEKFKKIRENEVRFEEINTADAEYLFVAYGLSARICQKAMDIAREKGIKIG
                     LLRPITLYPYPYKRLEELAAQVKMMLSVELNSGQMVEDVRLAVNGKVPVEFYGRMGGM
                     MPTPEEIVHHLETLIQYHHAN"
     gene            complement(15336..15665)
                     /locus_tag="PLO70_02095"
     CDS             complement(15336..15665)
                     /locus_tag="PLO70_02095"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQZ73269.1"
                     /translation="MEDNTNKTAAVKERKVKGRVEIDIQKCKGCELCTTACKEHALAL
                     SETINIKGYRYIIANNDACTGCVNCALVCPDAVITVYRTHPKKKPVDITPENIREQLQ
                     TMISPSK"
     gene            complement(15830..16174)
                     /gene="hypA"
                     /locus_tag="PLO70_02100"
     CDS             complement(15830..16174)
                     /gene="hypA"
                     /locus_tag="PLO70_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQZ73270.1"
                     /translation="MHELSIAHSILSIAERAVPAGSKVAVTAVSLQIGELSSIEIESL
                     EFAFSAIRSGTLLEKAELNIEIIPGEAACSDCGTVFHMDSFGTCCPHCKSFLVKILKG
                     KELKVLSIMVDE"
     gene            complement(16201..16671)
                     /locus_tag="PLO70_02105"
     CDS             complement(16201..16671)
                     /locus_tag="PLO70_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQZ73271.1"
                     /translation="MKTFFKALGNVFKKPVTTKYPFEQTFIPDDYRGLIGFDENLCIW
                     CRRCEMVCPPGAIVFSQDMEGKQTYHFNRAVCIYCGECVRACPKEGAIFQTAEPAPCA
                     LKAENINNGWNKLFDESLASREAYAAEKKRKAAEKAAADKAAAAKKEEENKTGN"
     gene            complement(16698..17786)
                     /locus_tag="PLO70_02110"
     CDS             complement(16698..17786)
                     /locus_tag="PLO70_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQZ73272.1"
                     /translation="MGRQYEIPIGAQHISLLEPLHFKFTTENEKIVATEANVYYVHRG
                     IEQACCSKFKFRQVSFVVGRVCGLCSISHTTAYSQVAEKLLKIEIPLRAKYLRMLVIE
                     LDRIHSHLLCLSHVAENSGFEALFMKTMQYREFSMELLEAVCGNRIQYDFSIVGGVAR
                     DLKPQVAQSILERLKKFIPQLDELMDIYKNNWTLSLKNKGAGAITKEDAMRLNVVGPF
                     ARAAGLAIDVRNETEDLLPWKEVGFEMITETSGDVYARNMVRLRELYVSIRIIENIIN
                     GLPESALITETKAFPDGEATVRLEAPRGELFYYAKGNKTQILERLKIKAPTFSNVAAM
                     AEAFTGNEYGSAPAIIASYDPCLSCTAR"
     gene            complement(17789..18130)
                     /locus_tag="PLO70_02115"
     CDS             complement(17789..18130)
                     /locus_tag="PLO70_02115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQZ73273.1"
                     /translation="MNKVMTSIENLKKDIGDFYKPELHHFMVMNAVEVGDQIEVQWFF
                     SDYAHPCDTTCFYTLVSPETEIPSIKDIVASAWVAEAELVDLMNIKVENTQKGFVLEP
                     DFESGPLRKKK"
     gene            complement(18127..18651)
                     /locus_tag="PLO70_02120"
     CDS             complement(18127..18651)
                     /locus_tag="PLO70_02120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQZ73274.1"
                     /translation="MNLASYRKKSPWILHYNAGGCNGCDIEILACLGPKYDIERFGMI
                     NTGNPKQSDILLVTGPVTKRSRERLVEIYAQMPEPKVVVSCGACACTGGVFRGMYNCE
                     GGVDQYIPVDVYVCGCGTRPEQIIDGVVQALGILEQKTKAMEVSVRLQKEPLKEEGQI
                     NRRNIADQIKPALS"
     gene            complement(18648..19487)
                     /locus_tag="PLO70_02125"
     CDS             complement(18648..19487)
                     /locus_tag="PLO70_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQZ73275.1"
                     /translation="MNDLLIPVLLILLAPLIGGLVYGFERIIKARMQRRIGPPLLQPF
                     YDFLKLADKRKMIVHSAHAFLGIMHFVSLWFALAVLLLGGDLILVIFLHLLSTSLIIL
                     AGYSTRSIFSHLGANRTAISILAYEPVLIIIAVSIFMLTGSFDASAAFNWDQPLLYKM
                     PLAFIALLLVLPIKLKKSPFDVAEAHQEVTGGVEIEFSGIFFEAVYTAKWLDYVFCYT
                     LVFLFAGNNILLGIGLMVFTFFYLNALDNSSARVNYKQMLRFSLTVSFSLAFINLLIN
                     IFT"
     gene            complement(19499..21364)
                     /locus_tag="PLO70_02130"
     CDS             complement(19499..21364)
                     /locus_tag="PLO70_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQZ73276.1"
                     /translation="MYPLITSLFLVPVIVSGLLLILPKTVARILVITSSLVLAALSLY
                     VFINLDGVLYFGVPHYVNDLVAVADIVLLVYFGWIALRRRSWLVGLMTVLQFGGLLYV
                     LKILPADHSLQFMVDKLSAFMFLLINVISGIIAIYSLRYIDEEHCSSFRKKHFLSILF
                     WFIAVMNLVVSSNNLEYFFLFFELTTLASFLLIGFRKDELSVKNALTALWMNQVGGVA
                     ILAAIFFIIYNGYGEATFSNLLAHASASGILLPLALLSVAALIKGAQLPFSKWLLGAM
                     VAPTPVSALLHSSTMVKIAPFIILRLSPALKDTPVATVVIALTGFVFVAAAISALSQD
                     NFKRILAYSTIALLALMIMMAAVGTPVTIVAALVLILFHGMSKCLLFLNAGILERVFH
                     LKQSSDMDRLGESGPFTSLVITIGFMSLLLPPFGAFIGKWLSIETLGALATDKKLLGA
                     LVIVAVAGGGAVLSLLYFKVMGLLIARTGDHDKIRFEKTGLVYAGTMYVLLGLIILSV
                     LGLPLLLTGYFVPVASQTLGMPVAVVMDGWNMHIGAMKLPMVPLLIALFLLPVTIITA
                     LFIRFKNVDRVKEYMCGEKINYSFSSFYFSTDRATPYFTTTGILFFVALIVAALI"
     gene            complement(21524..22000)
                     /locus_tag="PLO70_02135"
     CDS             complement(21524..22000)
                     /locus_tag="PLO70_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73277.1"
                     /translation="MKNIKTDKADILLIAIGNSGRRDDGLGWEFADKVSRSCGSLIDC
                     EYRYQLQIEDAVMVSRYDTVIFADASHTELKEGFEVKPCLAAPHYFFSSHMQSPETIL
                     YLARELYNKTPEAYTMAIAGHDWGLGKGLGKDAELNLQKAISFFEKEFVPAVRKTA"
     gene            complement(21978..23432)
                     /locus_tag="PLO70_02140"
     CDS             complement(21978..23432)
                     /locus_tag="PLO70_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HQZ73278.1"
                     /translation="MNRKITIDPVTRVEGHGRVTIHLDEYGQVKDSFFHIVEFRGFER
                     FIQGHPYWEAPVLVQRLCGICPVSHHLAAAKAIDQIVGIDPKDLSPTATKLRRLMHFG
                     QVFQSHALHFFYLASPDLLFGVDAPVEKRNVVAVAVENRELAIKGITMRKFGQEVIKA
                     VAGKKIHGILAVPGGVHKTLTENEREYFLDGKNIENIDTMIEWSQAMVEFMKNYHEQH
                     RKFLDDFAFFPSGHLGMVNKQGGLDLYDGLLRAVDSEGNRILDDISTDLYQDYFSEAV
                     AQWSYMKFPYLKKVGRENGWNRVGPLARMNVCDFITTPLAEKARQEFIAFTGKRVNNS
                     TMYSHWARLIEVLHCAEEIKILLHDPELMGNDLVRKGTPRSKGIGIIEAPRGTLTHHY
                     EVDEKGMITKCNLIVSTTHNNDAMNAAVKWVANNVISRKGHITDGMLNQVEVAIRAYD
                     PCLSCATQAMGKMPLKAELYNYNGELIDEKYKNG"
     gene            complement(23444..24718)
                     /locus_tag="PLO70_02145"
     CDS             complement(23444..24718)
                     /locus_tag="PLO70_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQZ73279.1"
                     /translation="MADMVNITIDGKSIQAEKGKNLMQVAKENGIFIPSLCYYEHIEP
                     PLGTCRVCTCKMNGKYNPACTEKVSEGLVVEVNTPELVDTRKALVEMMFAEGNHICPA
                     CEKSGNCDLQHMGYELGVSSTRFPHLFKDRIVDFEPSRMVMEHNRCIKCLRCVVDVKT
                     DDGKKVFTYQNRGNETYVGIDYDQEAKLTEEQAINAMKICPTGAIIVRGASLAAPFGD
                     RKFDMESVQKDYNKKKQTHRNRPPVMGKKIVATVSLAGCFGCHMSLLDIDTDLLDVLE
                     LVSFDKSPLTDFKEFTSRCHIGIIEGGVCNSENVETLRKFREHCDVLVALGECSVWGG
                     LPSMRNSIPLSECLEEAYLNSATSEQGASIIPYHEDLPKILDRVYACNEIVKIDYYIP
                     GCPPDANHIWKAVKNILWGEEYSILYSEFKYD"
     gene            complement(24723..26486)
                     /locus_tag="PLO70_02150"
     CDS             complement(24723..26486)
                     /locus_tag="PLO70_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQZ73280.1"
                     /translation="MPDDREKTLNEPIQTQMLDQLWELQRNNGCINDDDIKQLAHRFN
                     LSDIEVEGVVSFYHFFHRKPTGKYTIYLNNSIVADLKGFNRIKETFERETGATFGSVD
                     RTGTFGLFLTPCIGLSDQEPSALINFYPFTNLNAIKVKDIIHSLRNGATPASLCDAPA
                     DNIRYTPPGDKSIFFKKFTEGSAIANAAKLGRDGIIEELKKTELAGRGGAYFPTWKKW
                     KAAKDQPAVPKYVVCNADEGEPGTFKDRVLLNTQAKSLIEGIMVCGYTIGAEYGIIYL
                     RGEYHWLQPKLEEAINEYRKSGLLGTDCGNIKGFNFDIRIQMGAGSYVCGEETALLES
                     LEGKRGEPRTKWFFPVEKGYLQKPTVINNVETFCAAARIIEMGAEAYMQMGIPGSPGT
                     KLISVSGDCKLPGIYEIEWGMTVAELLELCEADDPYYIQVSGPSGECISIKEKYRRIS
                     MLDLLARKDIRCGGSFMIFNKQRDLVKVLLNFSEFFKSESCGICTPCRAGNFIIQRKL
                     ERLDNGLANEKDLEELKSWGTIMKNTSRCGLGKTATTSLIFSMEKFHDYFHNKLDKDF
                     NGLNLKFDMENATEEYEKYKI"
     gene            26952..28370
                     /locus_tag="PLO70_02155"
     CDS             26952..28370
                     /locus_tag="PLO70_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQZ73281.1"
                     /translation="MLQHKTNTNRLSYFFSLVKQSLRGDAHDYTQGSIRSAIVLLAIP
                     MILELSLESVFAVVDMFFVGKLGANAIATVGLTESAITIVYSIAIGLSTAATAVVARR
                     IGEKNPDAAAHAGMQSIVIALAVTVIISIIGIVFAADILRIMGASEEVVKEGTTFTRI
                     MLGGSVVIILLFLINGIFRGAGDAAMAMRSLWIASIINIILCPVLINGYGPFPELGLK
                     GAAIATVIGRGTGVLYQCYHFFRGKGIIKVKAVHFKWDTPVIKTLVEVAWPATLQFVI
                     QSGSWIVLAVLVAKYGGTDASAGYQVAIRNVVFFILPAWGLSNAAATLVGQNLGAKQP
                     GRAEKSVLLSTKYNAIFMAGVMLLFLLFSSPIISIFTSDPEVHRYGVQALQIIGCGYI
                     FYGIGMVMIQALNGAGDTKTPTWINFIGFWLFQVPLAYLLVNTFGWGPVGAFIAIPAA
                     ETAIAIAAYYYFKKGKWKEVKV"
     gene            28476..29519
                     /locus_tag="PLO70_02160"
     CDS             28476..29519
                     /locus_tag="PLO70_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQZ73282.1"
                     /translation="MNNVIDQNRLRQTFFIILILLMGVVLFIELYSFLPALLGAVTLY
                     ILMRRWMFYLAEKKKWKKGLAALLLMLISLIAILVPVGMLVNMLSGKVTYAVEHSTEL
                     VGALKKVASDFEQRFDIVILSDANINKLGTSLTQALPKILGATFNTLAAVFFMYFILY
                     FMFVSGRQMERVVYEYIPLKDDNVTKLEKEVNMMVVSNAVGIPVVAIAQGIVGLIGYL
                     IIGVKEPFFWFGVTCIAGMLPVIGAALAYIPLIIIFLANGQTWQGIAMIFFGFGVIGT
                     VDNVLRFSLLRKIGNVHPLTTVFGVIIGLNLFGFIGLIFGPLLISMFMLLVKIYSSEF
                     MTKQRDINKIMEN"
     gene            29733..30473
                     /locus_tag="PLO70_02165"
     CDS             29733..30473
                     /locus_tag="PLO70_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQZ73283.1"
                     /translation="MRKLILPISMGLLCTSFTIIPSHQTVNSHTVSVTPDLYTGSSYT
                     TAEADAATALYNDIDLQQYGLSKEAFDHAWKGYQHLVKKKMVSRSNYLTICDFSQSSK
                     RKRLYVIDIDNRKLITNTYVAHGKNSGAEYANNFSNKPESLQSSLGFYITANTYTGKH
                     GLSLRINGVDPGYNDKALMRTIVIHGADYVNESRVVNGGYMGRSWGCPAVPKKEATDI
                     ITTIKNGTCLFIYHPGKNYLLGSKILNG"
     gene            complement(30576..31754)
                     /locus_tag="PLO70_02170"
     CDS             complement(30576..31754)
                     /locus_tag="PLO70_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HQZ73284.1"
                     /translation="MKKNSAILGAAFLMATSAIGPGFLTQTSLFTQELVTSFGFVILI
                     SVLLDIGAQLNIWRIITVSEQRAQDIANKVLPGLGLFLASLIAFGGLIFNIGNIAGTG
                     LGLNVLTGVDTKTGAMISCMIALGIFWYKEAGKLIDIFVKLLGLLMIGLTIYIAFSSH
                     PPVAAALYRTIWPEKIDVIKIVTLVGGTVGGYISFAGAHRLIDAGIKGEKNLSQVTKS
                     SVSGILITSFMRYILFLAALGVVWQGIKLSADNPSASVFQSAAGEIGYKFFGMVMWSA
                     AITSVVGASYTSVSFWKTLSPVVRNREKTITSAFIILSTIVFIYVGQPVKLLVFAGAV
                     NGIILPVALAVMLIAANKAAVVNQYKHPVLLQVIGWIVVVAMTYMVFITIQQSIGKFL
                     "
     gene            complement(31833..32570)
                     /locus_tag="PLO70_02175"
     CDS             complement(31833..32570)
                     /locus_tag="PLO70_02175"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HQZ73285.1"
                     /translation="MQIDINCDMGEGIGREELIMPFISSANIACGYHAGDAETMYDTM
                     KLCSKYNVAVGAHPSFFDRVNFGRITMQLPVNELYELVTQQLIIFSEVAESADQKMVH
                     VKPHGALYNLSARDALVAKVIAGAVKDFDNRLILVGLSGSHSIMQAEAAGLKTSNEVF
                     ADRTYQDDGSLTPRSQVNALIEDTGKVMEQVLQMIKEGTVTTISGKTIPVVAETICIH
                     GDGTHAADFAKTIHDTLQKNSILISSP"
     gene            complement(32554..33549)
                     /locus_tag="PLO70_02180"
     CDS             complement(32554..33549)
                     /locus_tag="PLO70_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HQZ73286.1"
                     /translation="MSLKVIKAGILDTIQDMGRYGYQQLGINPNGAMDKYAMQIANAL
                     VGNDLQEAVIELHFPGSVFRFTQPTLIALSGADFSPSINGVPVPLHHCIAVNKKDELQ
                     FNKPVSGARAYIAVHGGLEIKEWLQSSSTNLKAGAGGFQGRNLYKNDELLLKSKNEIY
                     YLLHDNDFKILPWFAGFAANENSQEIFFLPGNEWEWLTEESKTIFTTTPFVITEQSDR
                     MGYRLGNTPLITLTNEEVVSSAVGFGTIQLLPDGKLIVLMADHQTTGGYPRVAHVITA
                     HQSRLAQMKTGDEIWFRLTDHQTAEELLVKQQQHLLQLQNACIFRLQEYLHANRY"
     gene            complement(33546..34298)
                     /gene="pxpB"
                     /locus_tag="PLO70_02185"
     CDS             complement(33546..34298)
                     /gene="pxpB"
                     /locus_tag="PLO70_02185"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HQZ73287.1"
                     /translation="MNYLNIPYRIFPLGDSAITVDFGNVIDEAVNNEVIGRFHQLKNY
                     PLHGMIEMIPAYSSLTIRYDVCTVRKNTTIDITAFDWMKNKLDELLQLPMSAPQTSSR
                     LLQIPVCYENGFAPDIKQLAAIKNLSVEEVIQVHTAREYKVYMLGFLPGFAYMGELDE
                     RIAIPRKAQPQITFPGSVGIAGKQTGIYPLRSPGGWQIIGRTPLKLFDADREDPTLFQ
                     AGDTVQFYSITENNFYEIQNSLSFKRGDWSKV"
     gene            34340..35371
                     /locus_tag="PLO70_02190"
     CDS             34340..35371
                     /locus_tag="PLO70_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HQZ73288.1"
                     /translation="MSRFNRRDSFNLFTKLTPRRAWNGLKVLSSYYISKWTKKPVQWG
                     YPISISFEPTTSCNLRCPECPSGLRAFTRPTGMLQKDFFSETIDQLHKELLYLVFYFQ
                     GEPYLNPGFLEMVQYASAKRIYTATSTNAHYLNDENAKRTIESGLDRLIISIDGTTQE
                     VYEQYRVGGQLNKVLEGARNIVKWKKELKSSKPFIVFQFLVVKPNEHQIEEVKQLGRE
                     IGVDDVWFKTAQVYDYEKDPNRLIPTLDKFSRYRKTATGYEFKGKLANHCWRLWHDPV
                     ITWDGLVAPCCFDKDAQHQLGDLKKKSFKEIWKNGEYSKFRQQILKGRKNIDICSNCS
                     EGTKVWSND"
     gene            35389..35877
                     /locus_tag="PLO70_02195"
     CDS             35389..35877
                     /locus_tag="PLO70_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQZ73289.1"
                     /translation="MQIPSHISTRLQYQHKTLVDLIDGLTEEQVRRQIIPGKWSIFEN
                     IVHLQTYQHTFITRVRQLLEQENPAFDRYTAEADPLFLDNCSKSVREIMHDMLTTRKE
                     IASGMLTLADTDFTKKAIHPALGAMTLLQWINFFLLHEAHHLFTILKLSGELKKVDNP
                     AS"
     gene            36234..36587
                     /locus_tag="PLO70_02200"
     CDS             36234..36587
                     /locus_tag="PLO70_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ73290.1"
                     /translation="MSDLRTNVDQLNQMILEGKILEAFDKFYADDVVMQDNDYPARSG
                     KEINRQYEEAFVNGLTEFRGAKVVNTLISDDLSVVEWWFDYTHKDYGVRNYTQLAVQR
                     WKGGKIAEEKFYYNN"
     gene            36678..37136
                     /locus_tag="PLO70_02205"
     CDS             36678..37136
                     /locus_tag="PLO70_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQZ73291.1"
                     /translation="MGIEKDIQQAKFRNAHQKAGINLIYTLAWLKEKTKLIFEAEGIT
                     SQQFNILRILRGSFPQPLSTLQIRERMLEKMSDTSRIVDRLIAKGLVKKVTCKQDRRL
                     VDVIITDKGKKLLDRLDTRQDEIDGVLSNLSEKDANILSDLLDKIRDGGE"
     gene            37179..38735
                     /locus_tag="PLO70_02210"
     CDS             37179..38735
                     /locus_tag="PLO70_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HQZ73292.1"
                     /translation="MKRPATWLLLIAFFSFYTTEAQPKYEFRGVWIATVNNIDWPQSG
                     MTDPEKQKADYIRLLDLHQQNGMNAMIVQVRPAADAFYPSQYEPWSQWLTGVQGKPPT
                     PYYDPLQFMIEETHKRGMEFHAWLNPYRAVHHIGQSSVAPNHISRVHPEWFLTYGDKK
                     YFNPGNKEAQAFVVKVVRDIVKRYDVDAIHMDDYFYPYRIPGKEFPDAASYAKSGSKL
                     SKDDWRRSNVDTIILALSKAIKEEKKMCRFGISPFSVWRNKDKDPVRGSDTRAGQTNY
                     DDLYADILLWLQKGWIDYVTPQLYLEIGHDKIDYAKMLDWWSRNSFGRHIYIGHGIYR
                     TAENQPKWKNPAELPNQIKLLRRYPNVQGSIYFSSKSFERNPNGWNDSLRDNYYKYPA
                     LLPPMRWIDNEKPWPPIVERSITNDSIIQLDVRPDPRNRVEIKYYVVYQYAADSHNET
                     FGNVPRYLSKFVTMPAGFQLKEPLSSKHFSYRYVITAVGRNNNESELSEIAILVQPGG
                     SKWSFYNPKP"
     gene            38815..40032
                     /locus_tag="PLO70_02215"
     CDS             38815..40032
                     /locus_tag="PLO70_02215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQZ73293.1"
                     /translation="MPGYELWSDKERKEVNDVLETGILMRYGFDGPRKGVWKSKELEA
                     AVNETFGSKYAQLTSSGTAALTTAMSALGIGYGDEVITPSFTFVASFEAILSVGAVPV
                     LVDVDDTLTLNPEAVRKAITPKTKAVMPVHMCGSMADMDALIAICKEHNLILLEDACQ
                     SIGGTYKGKHLGTIGDAGTFSFDFVKTMTCAEGGVVMTSREDIYIKSDGYTDHGHDHK
                     GVDRGADLHPFIGYNYRISELHAAVGLAQIKRLNEFLTIQKKNHTQLKNILSQVPEIS
                     FRRIPDPAGDSCSFISWFLPSEEITRSVVAELKAQGILAGNFYWFDNNWHYIRKWDHL
                     KNAITLNSLSPELKERVTQQANKDFTASDAVMSRCISTSISLLWTEEQIKEKGEKIVN
                     TVRMVLQNRAVTI"
     gene            complement(40037..41173)
                     /locus_tag="PLO70_02220"
     CDS             complement(40037..41173)
                     /locus_tag="PLO70_02220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ73294.1"
                     /translation="MKKVRHYLLLFLIVSGTVYDAFSQAEFAVDFKKKLDSLRIAGNF
                     PGLSAAVYYGTNNSFAAVSGYADIEKNILLQPGDCLMQGSVGKTYVSAMALQLVKNGK
                     INLDKKVSTYLGHYAWYYRIPNATDITVRMLMNHTSGVMRYEFKEQFTKDLTFFPDKV
                     WAPEDLLHYILDEKAPFKAGEGWDYSDTNYILLGMIIEQVAGEKYYDLLRDNILVPLT
                     LLHTFPSDNRKLKRLVQGYAGTNNEFGGKDKVIGEDGLFIVNPQFEWTGGGIYSTTED
                     LAKWGKQLYEGQIVDTTLLLSAAVPAKLGRDVKYGLGVIIRPTSLGVAYGHSGFFPGY
                     MTEMLYFPRHNMCVAIQTNSSDFKNLRISLLKCLMEIASVVKRN"
     gene            41708..43195
                     /gene="rpoN"
                     /locus_tag="PLO70_02225"
     CDS             41708..43195
                     /gene="rpoN"
                     /locus_tag="PLO70_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQZ73295.1"
                     /translation="MALSQSLQQRLLQKLSPQQIQLMKLLQVPTANLETRIKEEMEEN
                     PALELDEDKHEDADDIKDEFSDTAEEDYEEADGSNDEYDNIDVSEYVHDGDDEVGDYK
                     LRDDNYPEEDEGRQLPYRTEASFYETLIDQLGLLKLEEKEQAIAEQIVGSIDEDGYLR
                     RETAAIVDDLAFRQNIIATEVEVESIIKRIQQFEPPGVAARDLRECLLIQLKRQKDEG
                     AQVDMAIKAISKYFDEFTKKHYEKIQRGLNIDDDQLREVMQQIIRLSPKPGGNVGGIN
                     KAENYVVPDFFILNNSGKLELTLNSRNAPELRISEGYRDMLKDYDRGAKKDKRQKEAV
                     LFIKQKIDSAKWFIDAIKQRQHTLLSTMNAIMEHQYEFFLTGDETELKPMILKDIAEK
                     TNLDISTVSRVANSKFVQTEFGTYRLKFFFSESLSTDSGEEVSTREVKKILSNFIEEE
                     DKKRPLSDERLTEMLQEKGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            complement(43215..43562)
                     /locus_tag="PLO70_02230"
     CDS             complement(43215..43562)
                     /locus_tag="PLO70_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HQZ73296.1"
                     /translation="MQLTAKLIQLLPLQTGAGKNGPWKKQDIIVETEGQYPKKVCISI
                     WGDKISESLLQVGSMLSISFDVESREYNGRWYTDVKAWKVEAAGAAKIDKAQDDQVYF
                     DEGQMENKDDLPF"
     gene            43724..45127
                     /locus_tag="PLO70_02235"
     CDS             43724..45127
                     /locus_tag="PLO70_02235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQZ73297.1"
                     /translation="MVFASLIFLFLFLPLNLLLYYSTKNATWRNSILILFSLFFYGWG
                     EPIWITLMIFSALVDYVNGRVIEKYRGTIGAKIGLVSSLIINLGLLATFKYSSFIVEN
                     VNQLFGTSFNLPHFALPIGISFYTFQTISYSVDVYRNEVKAQKSFLKFLMFVSLYHQL
                     VAGPIVRYSHIAEEIDNRKEKLADISKGISRFCIGLFKKVVIANVAAELVAKYMDADA
                     SSLAVGGAWLGLIMFAIQIYFDFSGYSDMAIGLGWMFGFHYHENFKYPYTATSVSDFW
                     RRWHISLGTFFKDYVYIPLGGNKKHIYLNLFIVWFLTGMWHGASWNFIFWGLYFFVFI
                     ALEKAFLLKTLKKLPKFFSHFYALLVIIPGWVIFYFTDTNKLYAYIERLFSFGSGNLW
                     NVGLTSDLTAHLFWFIFAIIACMPVYHKAKAFFESSLKKPAIFETAAIAVNIVCIFLC
                     VAQLVGKSYNPFIYFRF"
     gene            45155..46420
                     /locus_tag="PLO70_02240"
     CDS             45155..46420
                     /locus_tag="PLO70_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HQZ73298.1"
                     /translation="MTKTENTINIINVTVFTLVLGIGGLASLSMKKQAVSEIENRQLT
                     PFPAYSDSALWNGNYFRQVDQYYADNFPLRNRWINVSGSFRNKFGFESGDIKIYEPAN
                     DAEANEITDTTKNTVNSGPLPDDGAVGEVKKRLFVFKNRAFEMFGGSTAMGKSYANVI
                     NSYNRLGIPGLQVYNLIIPVALEFELTEKYKKLQKPNRPAIESIYNELDSTIKKVWAI
                     DEIRKHREEYIYFNTDHHWTSLGAYYAYRAFCETAGLTPVSLDTVRYKVKSTFLGSLY
                     RLTRDPKLKENPDSVKYYLFRDSVRFYIGSSNSLTYWGKSKMYGEGANGSNSYSVFLQ
                     GDLPIVKMETQHNNGRKIAIVKESYGNAFAPFLVNNYEKIIVVDQRYYTGDFVAMLKK
                     EGINELLFINNIFAAHTPFHIEKIKRLKK"
     gene            complement(46505..46936)
                     /locus_tag="PLO70_02245"
     CDS             complement(46505..46936)
                     /locus_tag="PLO70_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HQZ73299.1"
                     /translation="MKKLLFAIVLFLAFHQGNSQKIDKAKMQATYDAIKAAGIRHPDF
                     VMAQCMQETGNLNCKKCCLRYNNLFGFYIKGNKCKKFNSDEECIAYYKTWQDKRYDKW
                     RAKHPKADYYHFLKHVKYATGDKYTGELKPKVAWVRKHLQL"
     gene            47055..47744
                     /locus_tag="PLO70_02250"
     CDS             47055..47744
                     /locus_tag="PLO70_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73300.1"
                     /translation="MNTVLMMNSKNQLHKTKEQGAGTPAIIRLLASFFSYLFHPVFIP
                     LYIVGFMVYEHPYLFAGFAPKEKILVVAQAFAMFTFFPLVTVLLLKALKFIESFHLAT
                     QKDRIIPLVACGVWYFWIWYVWRNLPDYPKPAVQLAMAVWITASLGLMANIYMKISLH
                     AMSLGVMVAFILLLAFSQPLNFGIYISVSLLIAGIVCTSRFIVSDHSAKELYGGLLVG
                     VISMLIASWLG"
     gene            complement(47763..50804)
                     /locus_tag="PLO70_02255"
     CDS             complement(47763..50804)
                     /locus_tag="PLO70_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ73301.1"
                     /translation="MAYSVIDRLLMSKGKFIHIFFEPALLLVISLSVPVYAGSQEPQL
                     ILPVGHIGGVREAIFSPDYKHIVTRDRFEVPRIWDTRTGYLLANLPGHADRISCIGYN
                     RDGTMLFTGSRDGTLIIWDAKKALPLVKLGVIKDGITSGAFNKQGTLFISGSGDKSAR
                     IWDISTGKLLLVLGKGEHESEVSSVQFNQNGDIVRTISYPGRHYWNTKTGMLLAKTVY
                     NDSLDRFVITKLTTKDTSFLSGESGQWDFTPVLPNPGRKLVYDIYRNDVLVTDSLGKK
                     IFTTLNGHTYMTRLLYYSPKGDTIYMEPDDRLFATAWAANTMQLKKTIAYKGITRPNV
                     HRGDANKNIIFNWDRSYSVVVQDVNSINDFNVWGNKKGSRLSQFRGNKKLPQKIQNAF
                     FSRDNTLLMTTMYNDREPAIIWDMATGKIVTKLPYNKEERITHGCFSNDKTLAAIFGA
                     DGHISIFDIGKNILVSTITTGTGDGINDIIGGEISPDNSMICFNDGWAAFKVYDIHSK
                     KEIWSLREPGNYVRAAAFAPDNKRMLLSTTASLVKMVDLKTKKILYHFFPVDSTEYFT
                     QVPAGYYQASPGATKLLHYVTKDLKVITFEQLDIKYNRPDKVLETIGSNDKALINAYR
                     KAYQKRLKKLGVDTAYFRDGYSVPECDFVNRDAIESEQNQEKLMLHIKGRDSTYLLGR
                     FNVWVNEVPVFGLKGISLLRRNSLSFDTTITIKLSQGENRIETSISNVNGTESYRMPL
                     TVNYLPAVKQKESVRFIGIGIDKFSESKYNLQYSVKDIRDLSKKLKEKYKGNIIIDTL
                     FNENVTINTVKALKQKLLQTTENDKVIVAYSGHGMLSKEYDYYLSTYAVNFEMPEQNG
                     LPYDELENLLDSIPARKKLMLIDACHSGEVDKEDLVTLATTSDSLIKGFKPVAYKKDE
                     KHLGLRNSFELMQSLFVNVGKSTGATIISAAAGTQFALERNDLKNGVFTYSIMEAMSS
                     HPSMKISELKNIVGKRVEELTKGLQKPTSRNETIAVDWNVW"
     gene            51048..52082
                     /gene="recA"
                     /locus_tag="PLO70_02260"
     CDS             51048..52082
                     /gene="recA"
                     /locus_tag="PLO70_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQZ73302.1"
                     /translation="MAKTEKTTDMSTTNSEKLKALKLTMDKLEKDYGKGSVMMLGDKA
                     ETMQEVISTGSIGLDVALGIGGLPRGRVVEIYGPESSGKTTIATHVIAEAQKKGGMCA
                     FIDAEHAFDSSYAKKLGVDVDNLLISQPDYGEQALEIADRLILSGALDVVVIDSVAAL
                     VPKSELEGEMGDSKMGLHARLMSQALRKLTATISKTNTICIFINQLREKIGVMFGNPE
                     TTTGGNALKFYASVRLDIRRMTQVKDGDEAIGNHVKVKVVKNKVAPPFRAAEFDIIFG
                     EGISKTGEIIDMGTELGIIQKSGSWYSYNSDKLGQGRDSVKQLMVDNPELAKEIEGKI
                     REKIKEMQTA"
     gene            52271..53176
                     /locus_tag="PLO70_02265"
     CDS             52271..53176
                     /locus_tag="PLO70_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73303.1"
                     /translation="MTTTSPKTSLLQSLHLLFGAGLLVSFFLPWVAWKDIKVSGYHMP
                     AGNFFKLSESNFGLGNPFPQFDFTFYFFWLIPVLVVAGIVIVLQNKKTSIPAFLSGAL
                     ALSLVTVYYFFSNTLVDLGVGNSAFDMLEPGAFITVFTAIGFILTTLPLHSWFKKMAW
                     IIIGPLLAWGAYKTGEQSVLSETFTHTDQVAADYTADAAGLIKEFLTNDTAANKKYLE
                     KTIIVNGNTSAIEILGDSTSTIKFEDSTGSYAIFSLEKSQFDKINKITRGEAVSLKGV
                     CSGSIYSEILGTTAITFKRATFNIK"
     gene            53194..53736
                     /locus_tag="PLO70_02270"
     CDS             53194..53736
                     /locus_tag="PLO70_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQZ73304.1"
                     /translation="MKKTTLVLALVFAAGAAFAQKKTTSSATVSFDATTPKDALPKAE
                     NKTVIGSIDTQTGAVAFEAAVKNFAFSNPKIQEHFNAPNWMSSDQFPKFTFTGTIDKV
                     NKVKFDKNGTYTVKVNGNLTVKGISKPSKVQGTVTVADGKIKVAANFKIKLEDYNITG
                     QPIEAGKIDKEPKITVSAEF"
     gene            complement(53804..56215)
                     /locus_tag="PLO70_02275"
     CDS             complement(53804..56215)
                     /locus_tag="PLO70_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73305.1"
                     /translation="MKKVIATAGIIMLVFFSLQAQKIDSALTVLNTQFPTEKIYIHYD
                     KEYYVAGETIWFKAYLYNDGKPDAVSNNFYLQFIDAKGNIISSGKYPVVGATARGNIS
                     IPDSLPQGNYYIRALTPAMLNNDESFVYKKNIVIFKPGVTPAAAKLSQTVSLQFFPES
                     GYLVDGILTMVAFKAVDQFGIPVEISGMIKTEDGITVAPFKTYHDGIGRVPFKPLAGK
                     KYIAETETAAGKRQFNLPEVRASGIILKVQDEKGGKKFQLVRGPKEKEQFENLLLVAQ
                     INNHVVYETEIAFEGYPSIIGHLITDSLPSGILHFTVFSDKGVPLAERLSFVNNEEYK
                     VKPDINIVKLGTGKREENIFELSFPDMVQRSFSVSVVDIPGGGLTDKENIYSRFLLTD
                     DLKGYIHNPAWYFNSKTDSTDLAMDNLMLTHGWRRFNWNKILAKEFPEKKFNDESLIR
                     IAGKVVDEKTNAPLTTGKLNIYIEAEDSTTQNQEGILSEQGRFILDSLVFMGKAKFYY
                     AYTDKQGKPKPALAITDENEMEKQVIKIPLLIPSEVGEKNLSGLPYNEEIARRYTYTQ
                     GFEEKIKELENVDIKSKSSKKPFDIVNEKYTTGVFRTAGKVNLDNINEPANDKSMNVI
                     DYIKNRIQQVEIQGGRFVNRKNMSLMSGQKWVIGLFLNEQPADMGFLRTIRVTDVALV
                     KFYEAGFVGVGSGSPGGALSIYTKEKFMDEQKPDKLSFFEANGYSITKEFYSPSYTTA
                     EAKQIGVDGRTTLYWNSNLFTDMETRTVKLNFYNNDFSKKFKVIVEGFDATGKLIHLE
                     REIGN"
     gene            56416..>58232
                     /gene="uvrB"
                     /locus_tag="PLO70_02280"
     CDS             56416..>58232
                     /gene="uvrB"
                     /locus_tag="PLO70_02280"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQZ73306.1"
                     /translation="MPFNLHIPFPPAGDQPAAIQNLTEGILNGEKYQTLLGVTGSGKT
                     YTIANVIQQLQRPTLVLTHNKTLVAQLYGEFRQFFPENAVEYFVSYYDYYQPEAYMPV
                     SDVYIEKDLSINDELDKLRLRATSSLLSGRRDIIVVASVSCIYGMGNPTDYENGIIRI
                     NKGQTISRQAFLHSLVNSLYNRTTVEFNRGTFRVKGDTVDINLPYVDYGYRITFFGDE
                     IEEIESIDVVSSKRIGKMENAAIFPANLYVAPKDILQQVLYEIQDEVAAQAEYFKREG
                     KYIEAQRLTERVNYDLEMIRELGYCNGIENYSRFFDRRRPGTRPFCLLDYFPKDYLMV
                     IDESHQTIPQVSGMYGGDRSRKLILVDYGFRLPSALDNRPLNFHEFESLQNQVIFVSA
                     TPDDYELEKCGGVVVEQVVRPTGLLEPPIEVRPSVNQIDDLLDEIDKRVTKGDRVLVT
                     TLTKRMAEEMDKYLHRINIKSKYIHSEVDTLERIEILRELRLGKIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNEKSLTQTAGRAARNVDGLVIFYADKMTDSMQKTIDET
                     NRRREKQIAFNIEHGITPRTVIKSTKDVFAQTSVLDIKG"
BASE COUNT        16657 a        12764 c        11702 g        17109 t
ORIGIN      
        1 tttaggtttt tattaatggt ataaacgccg attgcagatt gccctataat atccctttcc
       61 agaacataga tatatttctt ttcacctgat ttttccctgg tggctataca aattatatcg
      121 ctggctgaca gtgaaacttc cctgaaacca ttcattttta aatccttcac tcttatagtt
      181 tcaggatttg atttatacag tttacagtga ctgtcaagaa tattatcaag actattggaa
      241 agcattatac caaagtcagc ataagaatcc cgtaaccgga tatatcctgc gcctatctca
      301 tcaaataaat gcttgtactt tccctttgac agtgttgaac gaagcattaa ctgtttagct
      361 aatgagatat ttccttttct cacagaaaac aatcctcttt gctcaataaa atttgcgatt
      421 tcctcaaaac tggctgtgcg agaaggctgg ttaatgagtg caaccacaat ggcttcttga
      481 agaaaaagaa gatttttgcg aggcatttta tacttgtatc cccattaatt tctttggatc
      541 aataacagga agaattacag cacccaatga agtaccttgg gttcttgtaa atattatacc
      601 cctgattgta gaataagcta tgctaactaa tgtacttccc aatccaccat caatgttata
      661 ttttggttta tcatccgttt caactttttc aagaaaatca atcaggtttt caaccttaaa
      721 aacaatttca tgagtataag atccttttac attaaggggt ttatctgtct tgtctctagc
      781 ttcaatgccc actactaact gaagtccaat aatattatcg tcgggattta gaccagtacg
      841 caattcaaaa ttgaattgat gtcctactac ggcattaata tcagcgtcag ttgccccatc
      901 aatattacca cttagggttt taatattgag gagatgaatt ttttcagcat cgacttttct
      961 ttcaggattc atattgctat tggttcagga tgattttgtt ctttactata tatagaaatc
     1021 actttagtat cataatgaat aaccggaaca tgaccttctt gccttttttc aactatatga
     1081 tactccgaac tgtgtacact ttcttcaggg aaacttactc taacagttct tggggtgcat
     1141 attactgtag cgcctaaagc agactctatc tttgctatag agcgcaaggt ataattgtgc
     1201 atacctgcca gaaacttact aatctcagct tcacttttac ccattttatc agctaggtct
     1261 ttttgcttca tgcccttttg ttccatgatc cagtaaataa aatcggcaat ctccaatgat
     1321 ttatcaacaa agattttgct gtcatcagac attgaggaaa ttgattcctc aaattcagga
     1381 atggcctcag ccttaagtac tttctttttc atacacgttg ttcttaaata tttaatacaa
     1441 aatcatcgtc cattaaaata tcaaacccgt caatttcaat tctatctttg gtttttgctt
     1501 cataaatcgc atctgatatt ttatttgcaa aatcaaaatg cggtttacaa ttaaggcaat
     1561 cttttatgct ttgtgctgtt tttctatctc catttaaaag aatgactact tcctcactta
     1621 tcctgacaca gtaaagccgc agcccaaagt ctgtttcacc atcactatca agaaaacgat
     1681 aggttgccgg aggcaatctt tctgcaaaac cttctctttt aaaaaaacga tcttgtgcac
     1741 catgcttttt accaatattt tcaataaatc tgtttatttc tgcaacttgc tttttgtctt
     1801 tctcattatg ttgcatcctg gtttggaagt cacggaattc ggataggggt ctcccttcca
     1861 cctgcacagt gtagtacgag acctttgaaa attgaagttg ttcaagataa agtatttgga
     1921 caatcaagtt aacttataag ttaagacaaa cataggtttg gaatgttaaa tttccaagtt
     1981 tatcgggtta aaaattatat atcgttgatt atcaattaaa aacttacccc tccgccgaca
     2041 ttgtcaccac atcatttatc tcatactctt taccctcacc agtaaaagcc tcttttaaaa
     2101 ccgcctgcag cagttgccca gcctgcatct ggcttcgctg cactcattcc tccaatcatt
     2161 tcctatttat ataaatttct cttctttgac taatacacgt tgtgttcaga aattaaggga
     2221 aatggaatcg gatttgattc ttttccccat gcagttaatt tactccgaaa gtatattttg
     2281 cctttagtta aagaaacatt tgtcgagtat ctgcgactca atatcccaca ccgctttaac
     2341 caatgcaggt gtgatgtatt tggtgcagat gtctcgttca gacaaggttt ttttaagact
     2401 caatattccg ggctttatag cacaaaataa gcaaagctga acagaattac cggccgataa
     2461 caatcccata aaattttttg cccgtccaaa aatatcgtcc taactttcaa caacaggaat
     2521 tatacacaga caggatcttc atttcttcca cgcatcttcc ctacgcaaac cactttgttt
     2581 ccgccctgtt tttttgtacc aataaacaat tggtgaatga acaaaaaaat aattgccgcg
     2641 gtatgtgccg gcttcttttt ttcgatggcg gctgtttgcc agaccgtact gtttatcaat
     2701 gcagggtcca gggcttccta tggacagcaa caggaactgc tggtggacgc aaacggtaac
     2761 tgccgctacc gcctgggcac cgtgaacggg ccggtaaaag attcctcttc ttttgcactg
     2821 ctgccggggc aactggactc cctgttccgc caggccgttg caacaggttt cttttcttta
     2881 caacataact atgatggcgg cgctgccgat ggcgccggaa tctatatttc gatgaaccat
     2941 gccggacagc aacactatgt gcaactgaaa aataccgatg tgccggccat taatcaattc
     3001 attacccttc tgaatactat gctggccccg cggcagatcc ggatcaacta tggacagttt
     3061 gtacccccca ggccacgatg aaccgctttt attatattac cagcatcgct gtctttttta
     3121 tgctgctgca aagttgcacc ggcacccgcc gctttggcac caccatcgaa cgggatgaaa
     3181 ataatccctg ccaggtgaat atgatcattc aggtagccat tgaaggcacc gatgatgatg
     3241 tgaccatggt gaaaagtgcc ctggaggatt gttatggtaa ggaatgtttt attccctgcc
     3301 ccggcgacag cctgaaagga tgcaaaacaa aaataaccgt ggtggtaaaa aaatacagca
     3361 gcctgaaaga agacgaaacc gccggttttc attatgtgca gatgatagat gatgacggcc
     3421 tgccctcgaa tgcctatctc ggcacaccga acaatggcgc ttccagcggc acctggcgca
     3481 ggaaccagcc ccccggtgtc tattgtcatg aagtgctgca tttctgcggc ctgcctgata
     3541 aatattgttc ccggctatac gaccccgtta ccgattctgc cagaacagaa ctggtatgtg
     3601 atccgccacc agaccccaac gggtattgct gcgccccatc tgcctcgcat acccgctgca
     3661 gcactccctg cgccgggcat gagcatgacc tgatggcgaa tatttataca ccactgtcct
     3721 gtgataatat tatggatgtg ctgaaaggag ccgggctgaa caactgcccg ccggaatgct
     3781 gtgcttctga taaaacattt acccgtccgc cggatgaagt atacatcatc cccggctatt
     3841 atcattttgg tgataagaac accaaattcg gcagcattgg tggaagtgtg ggctatacca
     3901 aacagatcgg cacttcgctt ggcgttacta tcgaaggtgg ttattacctg cattcagata
     3961 aggataacag ttataaacaa acatcggggc tgatgcatat caccggcggc attacgtacc
     4021 gggtgaccga cccgccaaca acggagaaag ggatcagtat ttccacccat gctttattcg
     4081 gggtgtcccg ctacactcaa aaaacaagct ttggtggtaa caccctgaaa gatgatgcta
     4141 cttctttgca tttcaatatc ggcgctgcgg ttgacctgca actgaaccgc tcctgggctg
     4201 ttcgtttact gcaggcagac tatgctccca cttttttcta tgaggccaca cagcataatt
     4261 accggatcag tgccgggctg gttcaccggc tgggacgata acaaaagaga gacgctttag
     4321 gattgatacg gctgagtacc ggttggcgat ctgcgaaaaa ggaaagcccc tgccgggtct
     4381 cataccggaa caggggcaat ctggtttaat ggttataatt atttctttcg ttttcttttt
     4441 ttcttcagga cagcctgcat gcggttagcc agtttttcaa tacccatctg cacggtgttt
     4501 tcgatctttt gtcctgccag cccggtgaag cgtcgggtaa ggtaaccagc cccgagactt
     4561 accagcccag taaataaagt gttctcacct tctttgcccg tggaagaggc tgccatctgg
     4621 ttgcgcagca aggtaccggg gcgcaattcc agcttggtct cgttccagtt ttgctgcagg
     4681 tttttttcct gctccagctg ttgtatgcgg agccgcatct tttcttcctg tatatctttt
     4741 atatgtttaa tttttttcat cgtcttcttc cggttcaaat aaggcttcta tcagtttgtt
     4801 cataatgggt atgcgcagca ggcggtctct ggacaaccat aagatcaacc ccaccaccag
     4861 tataaccccg gctacaatca caaaccccag ccacatattc tccagccatt gcccgatgaa
     4921 gatggcggct gcgatgctga gtattaccag gaataaaaag aataccaggg ccgccatcag
     4981 catagctatc atttcagcgg ctagctttga cactttttcc gccatggata actttacctg
     5041 cgataccctg gtattagcat acgctttgag ctgttcgatc atcgactcta ctttgttaaa
     5101 ctcttctgac atgtcttaaa gttacaatat ctgtcagatt acttcgtcga gctcgccatt
     5161 gatccgggcc atttgttctt ccattttgtt tttgcccagt tttactttgc gggtaaactg
     5221 atcttttaat tttttaccgt tttccgcaat tttatgacgg gtgttctctc ctttatctgg
     5281 tgcaaataat acacccatga gaccaccgat agctaatccg gctcccagcg caatgaggat
     5341 ctttgaagtg tttttcatac ccgttatttt atgagtgatt aattattttc cttctcttca
     5401 aatataactt tccaaagggc tgaaaacaat gaggatgttc atagcggaga atgactgctg
     5461 tcaatttttt cggtaaggag cagaggcgaa cttgcgttat tgttttaatg atttcttttc
     5521 actttatgcc atccccggca acagtaaact ctctgttctt ccctatacag acctgtgtat
     5581 aataaaagcc ctgccggaaa caacattccg gccatggtta agaaaaagac taccttacca
     5641 tatctcttaa ccaaccaaaa ttttttttta tgaaaagtaa gtttctaatc gtagcactgt
     5701 ccggactttc tgccttgttt ttttctgcct gtactacacc agcagaagaa gccaaaccta
     5761 ttgacatgga taaactaaaa gtcgagatcc aggccctgga agatgcctat gctgctgcag
     5821 aaaaagccaa agatgctgat ggagtagtgg cctattacag ccaggatgcc gttagttata
     5881 gccggaatga agcgcctctt tcgggagctg ccgctatcaa agaaaaaatt gccaagcgcc
     5941 tggccagcga caccaccggt aacaccagtg tctataaagt agtggacctt tttgcagaag
     6001 gcaattcagc tacagaaatt ggttcctgga ccactattaa tcctgcaggt gcagaagtgg
     6061 agaaaggata ttatatgtca ttctttcaaa aacgtgacgg aaaatatgtt tgcgtcaggg
     6121 atatgaatgt gaccaccacc cctgccaagt aattgctgta agcaacagga tgtaatgaat
     6181 agtatataat tcggagaggc tgtttcaaaa aacaaacaaa tttgtcacat tgagtttatc
     6241 gaaatgcgta caaatttgtt taccaatcat tgtcatattt cggctttgct caatatgaca
     6301 atgattggtg tttttgaaac agcctttttt gttttgctgt tgatagtgta aagaaacacc
     6361 ttatgatttc cttcctgcag acattaataa gtttgacaac tgatgactgc attacactca
     6421 atcagtgttc attcgtgtca gtttgcagca accattaatt ataatttcat cggttgtttt
     6481 ttccaatagg tcagtgaacg ccacaaccat tcaaacgggc caaagcggaa gtaccgtaac
     6541 cagatacttg aaaaaataag ctggaatatc cataccgcaa ataccacata atagagctgg
     6601 tgaaatttta gtttgttgta ataccctaat ccataaccat agaagaataa tgtacagaga
     6661 atagattgca tcaggtaatt ggtaaaagcc atctgtccca cattggccag tgccctcatc
     6721 agccacggaa tgatgccact gcggaatatg agcatgacca aactcgcatg cccgatggta
     6781 atgaggatac ggcgaaactc tgcaaatacc atatgcgatg aactccagct atcggcatac
     6841 aggcccatcc ttccaaagct cataaagcct ttgtcgaata tagcccaggc aatgggcaca
     6901 ccaatgccat agccgataag caaccacatc atataagtgg aggttcgcaa tcgattggag
     6961 aaaaaccccc aaaggtataa ggccataccc aaaaacatca tgaccagcag atcccacact
     7021 tcattataca tataccatat ttcaccaccg gcattgcggg gaatgaaata agtaaagatg
     7081 gtgccataac cggactgcat tttgcggaga ttttcattgg taatggcagt gtcaggcttc
     7141 tgacttttct ccatctctgt ccacattttg atggcgccct gttgttccgg ggtcagctct
     7201 ttcttttctt tctttgctgc catcgcttca gtataagatt ctcttttctc ccgggtttca
     7261 ttccagtccc attgtttttt taccatcccg aaagccacac aggtcagcgc tattactacc
     7321 agccacttgg gtgacaattt tctgaatgca aataataaca taccgcatag cccgtaaaaa
     7381 aataaaatat cgcccggcca taacaataca aatgcattga tgaccccaaa cagcaccagc
     7441 cataataaac gccggtaata atattctgct accgtaggac ggccgggtaa ctcatgcttg
     7501 ttttgcataa acaacaccat cccggctcca aataacatgg aaaacaatcc ccgcatggtt
     7561 ccttcaaaaa aaacaaacac aatagcaaac gtataataat cttttcctgc cctgggcccg
     7621 gtgagaatgg aattccagaa atcccagttg ataccaaagc ccggaatgtt catcatcaat
     7681 attcccaaca aagccacccc acggatcgta tcaatacttt taatacggtc ggccctggtt
     7741 acaggcctca ccgttgattg ttcaccggca ccaccagtac cttgcattgg aataactaat
     7801 gattgtggtg tgccggaaga attatgctga tccatataaa tggtttaatg gttgtaaaaa
     7861 tcagagagct ggtgaatcgg ttatagcatg gggttctttt attttccagg gcagccattt
     7921 gcggtatatc aaacaccgcc ataaccactc cagcggaccc atggtaaaat aacggagcca
     7981 gaaaacagag aagactattt gcaccagggc tatctctgct accataaaat acaattgcca
     8041 ctgctgcaac ctgccgaaat aaccaaatcc atacccatag aaaaagaaag tgcagatgat
     8101 ggtttgcatg atgtaattgg tcaaagccat tttacctgtt gcggccagtg cctgccaaag
     8161 ccagttcagg aatttactgc gcaacaataa cataacgagg gatgcatagc cggtggccag
     8221 caacattctt tcgatgggga agaactggtt aaaaggaacg gggtattttt caatataccg
     8281 ggcatagtcc gccagtttta tactctgaaa atgaatccgg aaccaggcca gtgtaaaacc
     8341 aatggcgaga gctaacactg caataagcac atacctggaa gaagccatgc gatgattgaa
     8401 aaaaccaaaa ccaagtaagg ccatgccgag cagcatcatg gagctgatat cccaaacccc
     8461 ggtacgatac aaccagtttg attctttgtt ttgcgaccgt tgcatcaggt gtttccatac
     8521 tttgccgtaa gaggcccgga tggctttgtt ctccgcaact gttttggagg aatcatattt
     8581 caggtctttg atcatacctt cccacatgcc tttttctcct gcctgttttt ttgtcagtgt
     8641 atcattcttg cgggccagcg aatccattaa cttatttttc gccagtgtgt cttttagcaa
     8701 gaccgtatct tttgttctgt cgaagctgtc tttttttgcc cgggccgtac tgtcctgttt
     8761 gaatttcttt tccactgctg taacagcgag gaatttttta taccctttct tatcatccgc
     8821 ataattccag tattgtttgc cgcaataaat cagtgtacac aggatggcag cgatcaataa
     8881 cccccttgcc ggcattcgcc agaaagcaaa taataaaatt ccaactacgc catactggaa
     8941 taaaatatcc ccgggccaca gcaatacaaa tgcattgaac accccaaaga tgataagcca
     9001 gatctgcctg cgtatgtagg catcaggtgc agccagcggt accgggtgtt cttttttctg
     9061 cataaataaa ataataccgg caccaaagac cagtgcaaag agagcccgca tctttccttc
     9121 gaataagatg gaaacgatgc tgagtaattt gtaattgcca ccatgtgttc cctgccggaa
     9181 aaaggtttgc tcattgctgg taaagccgcc aaattcccag atgctgatga caagaatacc
     9241 cagcagcgca ataccacgca acacatctaa agaaaaaagc ctggataaat taacgggttc
     9301 attggctata acctgatcag gggcagtgcc cgaaggagtg gaaggatgga ggctcatgtt
     9361 ggttggtttt gttcgtttcc ttcaggtact tcaactaaat ataaatattt attacgggaa
     9421 gaatagaaaa aattctctag cgcagttacg tttcatcagc catatatagt tttttataca
     9481 aggccctgca tttttccgag cagaaataat gtattgtatc atcatgtttt aactgtagag
     9541 aagttgccgg tgatttaagg agcatgtgac ataccgggtc aatcacaaat gttttggaga
     9601 gatttgttat acaacaatcg agttcaaata agtcaacctg ttgcagcagg tttttaaact
     9661 ggtgtaaccc gattttcttt actgcaaagc tgtgcccctg cggaagctgg tccaggaaat
     9721 ctgctgaaca aagtattttg cctttatcgg cggccgacat aatgcgggct gccgtattga
     9781 tagtggaacc gaaataagca ttatcctttt ttaccaccgg gccatagtga agggctgcat
     9841 gcaggggtaa aaaatcttcc tgttcatgag cgaattcaaa cagaaaagta gcagtgtaag
     9901 ccagttgttc tgcctgggaa gaaataataa ccacctggtc tcctatccgt tcatgcaggt
     9961 ggctgtcacc caccagggac ttattaacca gggtaagata cctgtctacc atagcagcgg
    10021 cggactcagc accatgtatc tctgtcatgg cggtgtagcc ggaaagatca gccattaata
    10081 tagcaatgtt tatttccatt tcaatttgct gattaaatgt attaagaaaa gatgaaccct
    10141 gttacagtcc cggtatttca acaaatcttt catctgcggg ccgggagcaa cttattcctt
    10201 cctattttta ccgttcatgt cactctatat cacttccctc aattccggca gtaatgccaa
    10261 ctgttattat atcggcaata acaatgaagc agttttaatt gatgccgggc tgagttgccg
    10321 ggaaacagaa aggcggatga aacaactgga gctgtcgatg gaaagagtga aagctatttt
    10381 tatttcgcat gaacatgccg atcatattac cgggctggcc ggtatttcta aaaaatacca
    10441 gcttcctgtt tatatcaccg acagcacttt ggtaaggagt aatatgcctg ttgaaccgca
    10501 actggtcaat tcttttaaag atgccaaacc cattacaatc ggaaacctgg ccatcacccc
    10561 tttcaaaaaa agtcacgatg cagccgatcc gcacagtttt atggttgctg gtcatggggt
    10621 gaatgtaggt gtcatcaccg atattggtta tgcctgcaaa cgggtaataa aatatttcag
    10681 ccagtgccat gctgtttttc ttgaatccaa ttattgtgat gacatgctgc gcaatggtaa
    10741 ctatccttat catttgcaaa aaagaatcag cagcgatgaa gggcatttat ccaatgcaca
    10801 ggcattggaa ttatttcagc agtatcaatc agctgatctg cggttgctca tcttatcgca
    10861 tttatctaaa aacaataata agccggaact ggtggaggca atgttcaacc agcatgcggg
    10921 tagtacacag atcgtagtgg ccagccgtta tgaggcctcg cctgtttttt gcattgaggg
    10981 tattgcagta acaactgctg caaaaccgaa acggcgaatg atcataaaag atgaaaggca
    11041 gttgtcgtta ttctgaatga gatctcctgc aaccggtaat tttattttcg gcggtataga
    11101 ttaagcgtta cagaagtggc aaagaatggt ttttcactca gggtaagaaa ggctgaatta
    11161 tcgtttttaa aacaaattgc ttcgccctgt ggctccagca tatatcctaa agccaggggt
    11221 gtacgcagca atgctgctcc aattgtttca ccggcattcc ttttccagta atagacattg
    11281 gtataggttt ttaccagcaa ctcttttcca tcgggagaag cggcagcagc tgaaactcct
    11341 gtaaaaggca tttcacccac cagggtggcg atcatggtgg tagtggtgct ttgcggatag
    11401 ggtaatttat agatacggga agcactatct cttttactta tgaggtaaat atcttttgta
    11461 ctgttatcaa gcagcatcgc ttctgcatca tgtgaaccat cgggatattg gaatactatt
    11521 ttatctgtat taaagacggt atcggttgtt aaggtaggtt ctgcaaatcg atagatggca
    11581 taattggtat aagaagcact gttatcccct gtttcagcaa ggtacacata attggtgccg
    11641 gccaccgggc cattgcccac ggccatatct tcccagtcgc ggttggtcac tccttttacc
    11701 ggaatttttt tctgtacagt gccggtataa gaaagcagga ataactccgg cggattgcct
    11761 ccatcctgtt gcacccagat aaaaccggtg ttgcccttac tgtcagcaat accggaggct
    11821 tcatcaatgg tacccgggag aacaggtgtg gttacaggta ctgcttcaaa aggcagcaca
    11881 gttacctgtg cagaattcac ttccttctgg cattgcataa aagcaaaaca ggtagtgatg
    11941 aataaaaaaa ggctccgcat gaaacgcata ttgagttgat agtacaatca ttttcaccca
    12001 accggaaaat gttgccagct tataaaaata taaaaaataa ggggaacgct tagtactgta
    12061 ttgtttatgg agccagtctt tctgttttcc attgaccctc tgcactatct gctaccggag
    12121 tgtataagat cctgtcgtgc atgcggctgg ggcgtccctg ccagaattca attttggtgg
    12181 gcttcacccg gtaaccaccc caatgcgggg gtttgggaat tttgccatgt ttgaaacgct
    12241 ccgcataata ttcaaatgtt tctttgagcc aggtcttgcc ggctacaacc acactttgtg
    12301 gcgatgccca tgcaccgatc ttactcccat ccgggcggct gtcgaaatag tcgatgcttt
    12361 ctttgactga aattttttct gctatcccat taatgcgaac ttgtcgttcc agttctttcc
    12421 agaaaaataa taaacagcaa ttggcattaa aatccagttg ctgactcttg gcactgttgt
    12481 aattggtaaa gaatacaaaa cccttttcat caaacgcttt cagtaatact gtacgggctt
    12541 ccggcatacc atcatgaccg gtagtggcca gggtcatagc attcatctca tcgatctgtg
    12601 atttttctgc atcgttccac cagcgctgaa actgtgcaag gggtgacgga tccacctcat
    12661 cttcattcaa cgtatgcaat gtataatctt tgcgcagctg cgctatttca tctggtttca
    12721 tgtatctgca ggtatttacc ggtcaaagat aatccaacat tacattaccg gttctttctg
    12781 ttgcaccact aatgctttgc ctttttccag tgcctgttta ttaatgtcaa gcaggtgatg
    12841 atatttttcc ggcaatactt tcttcaatgc ttcgaggatg gaatcaattt ttaccaccgg
    12901 cttcaattgc aaataagcgc cgaggatgat catgttcatg atctttgcat tcttcatcaa
    12961 taatgcttcg gtactggctg gtataccaat gatctctata tccgttcttg tacttggttt
    13021 aaagatatta ccagattcat acaacagcaa tcctcctggt tttacccggg gtgcaaactt
    13081 atccattgat ggctggttca gcacaatggc tgaatcaaat aaagaaatga tgggtgagct
    13141 gatctttttc tctgatataa tggtaataca attagctgta ccaccccgca tctccggtcc
    13201 atacgatggc atccaggaaa tttcttttcc ttccaccatg ccggcataag ccagtgtcat
    13261 acccaatgat aatactccct gtccgccgaa accggcgact atgagttctt ccagtttttt
    13321 gaaatcattc atgatactca cattggttaa ttaaagaaga attgttttag ttcatttttc
    13381 ttgtcttgct ttgataaact cagcatgaca agaaaaatgt cagcctgagc ttgccgaagg
    13441 cgttattaaa tcaaactcct ttcggcactt taatatctcc cagtggaaat gtttttacca
    13501 tttcttcatc aataaacttt gatgcctcca cgggtgtcat cttccagttg gaaggacagt
    13561 taccgatgat ctctatcaaa caggtgcctt tgtgcaactg ctgatactta aatgcattct
    13621 gcaatgcttt cttggtacgc cgcactgcat tagcgctgtt cactgcctgg cgggtaacat
    13681 aaaatgctcc gggcaggttc gccattaatt ctgatacttt gatgggggct ccataataac
    13741 ttacatctct tccatcagga ctggtagtgg tgtgcatgcc tgccagtgtt gttggcgcca
    13801 tttgtccgct ggtcatgcca tacacagcgt tattcataaa tacgatcagg atattttctc
    13861 cgcggttgat tgcatgcaat gtttcggcaa taccaatggc tgccaggtcc ccatcaccct
    13921 ggtaagtgaa tacatattta tccggcatca gttttttgat acctgttgcc actgcacagg
    13981 cccggccatg ggccgcttcc tgcatatcta tatccatata ctcatacgca aatacagaac
    14041 agcccaccgg tgcaatgcca atggtttttt cctgtatatc catttcctcc actacttcca
    14101 tgatgagttt gtgcaccaga ctgtgagcac aacccgggca atagtgcata gccacattca
    14161 ccatggtaga gggcttgtgg tataccattt catattcttc ctcatcgtgg caggccggtg
    14221 ctattacatc cagcgccgta tcgggcaata catgttcttc agttggcatg atgatactgt
    14281 attaaagttt ctaaatgatg aacaatttct tccggggtgg gcatcatccc tcccattctg
    14341 ccgtaaaact caaccggcac tttaccattc actgccagcc ttacatcttc caccatctgt
    14401 ccgctgttga gttccacact cagcatcatt ttcacttgtg cagccagttc ttccagccgc
    14461 ttgtacgggt aagggtataa agtaatggga cgaagcaaac cgatcttgat tcctttctct
    14521 cttgcaatgt ccattgcttt ctgacaaatt cttgcactca atccataggc aacaaaaaga
    14581 tattccgcat cagcagtatt gatctcttca aaccgcactt cattctctct tatctttttg
    14641 aatttttctt ccagtttcag gttatgttgc tccattcttt ccggctgaat aaacaaggat
    14701 gtgatataat tcctgttacg ggtctttggt ttgccggtag tggcccatga cttatcaact
    14761 gcaggccttg tatatggttt gaactgtact ttctccatca tctgtccgat tgccccgtca
    14821 gataacatca gcacagggtt acgatattta tctgcgaggt caaacccgag ataaacaaaa
    14881 tcagccatct cctgcacaga agccggtgcc agtacgatca ttttataatc accatgaccg
    14941 ccgcctttta cagattgaaa ataatctccc tgcccaggct ggatagttcc gagtccgggg
    15001 ccggccctgt ttacattaac gatcaaacat ggtaattcag caccggcaat ataagagatg
    15061 ccttcctgca tcaggctgat acccggtgag gaggacgagg tcatggcacg aaagcctgca
    15121 ccggcagcac catagaccat atttatggaa gccacttcac tttctgcctg catcacaatg
    15181 cggttatgct tcggcatttc tcttgctaaa tattccagca cttctgattg cggggtgata
    15241 ggataaccaa agtatgcatc gcagccggcc agtattgcgg cttctgccat ggcttcatta
    15301 cctttcatga gtttagtttc cgtcataacc tgtttttatt ttgaaggact gatcatggtt
    15361 tgtaattgct cccggatatt ttccggtgta atgtcaaccg gttttttctt tggatgggta
    15421 cgatacacag tgatcactgc atcgggacaa acgagtgcac agtttacaca accggtacag
    15481 gcatcattat tggcaatgat gtaacggtat cctttaatgt tgatggtttc ggaaagtgcc
    15541 agtgcatgtt ccttacaggc tgtggtacag agttcgcagc ctttgcattt ttgaatatcg
    15601 atctcaaccc ttcctttgac ttttctttct ttaaccgcag cggttttgtt ggtgttgtct
    15661 tccattgtcg gggtttttgc ttttttatcc agaagccaca aatgaacatt tgtttggttg
    15721 ctgaactata acaaatttcg aaagaaggtt ctccttatta taactatacc gtcataggcg
    15781 gatatgatta ttgtcagttt tccggagccg gaaaccgggt ttgccgggct cattcatcca
    15841 ccatgatact gagtacctta agttcttttc ctttcaggat ttttaccaga aagcttttgc
    15901 agtggggaca acaggtccca aaggaatcca tatgaaaaac tgtaccacag tccgagcagg
    15961 ctgcttctcc cggaatgatc tcaatattaa gttcagcttt ttcgaggagg gtgcccgatc
    16021 ggatggctga aaaggcaaac tcaagggatt cgatctctat actgcttaat tcgccgatct
    16081 gtaatgaaac agcagtaacg gccaccttgc tgccagcggg gaccgccctt tcagctatgg
    16141 ataagataga gtgtgctatg gaaagctcat gcataacaat taaactaaca gattaagcgc
    16201 ttagttacca gttttatttt cctcttcttt tttggcggcg gcagctttat cagcggctgc
    16261 cttttctgct gccttccttt tcttttcggc agcataggct tcacggcttg ccagtgattc
    16321 atcaaataat ttattccagc cgttgtttat gttttctgct ttcagtgcgc aaggcgccgg
    16381 ttctgcagtt tgaaaaattg ccccttcttt gggacaggcc cgtacacatt ctccgcaata
    16441 gatacaaaca gcccggttaa aatgataggt ctgcttccct tccatatcct gggaaaaaac
    16501 gatcgcaccg ggcggacaaa ccatttcgca acggcggcac cagatacaaa gattttcatc
    16561 aaagccgatc aggccgcggt aatcatccgg aataaaagtc tgttcaaacg gatatttcgt
    16621 tgtgacaggt ttcttaaaaa cgttaccgag tgctttaaaa aatgttttca tcttctttct
    16681 tttgtataat cagtttctca tctcgctgta caggacaaac atggatcata agaagcaata
    16741 atggccggcg cacttccata ttcatttcct gtaaaggctt ctgccatagc tgccacattg
    16801 gaaaatgttg gcgcctttat cttcaaccgt tccagtatct gtgttttatt tcctttggca
    16861 taatagaaca attcacctct cggcgcttcc agccgcactg ttgcttcgcc atcaggaaat
    16921 gctttagttt ctgtgatcaa tgccgattcg ggtaaaccat tgatgatatt ttcaatgatg
    16981 cggatagaaa catagagctc ccggagccgc accatattac gggcatatac atcaccggag
    17041 gtttctgtta tcatttcaaa gcccacttct ttccagggca gcaggtcttc tgtttcatta
    17101 cgtacgtcaa tagccaaccc ggccgctctt gcaaaaggac ctaccacatt cagccgcatg
    17161 gcatcttctt tggtgatagc accggcacct ttatttttta atgataatgt ccagttgttc
    17221 ttgtagatat ccatcagttc atccagttgc ggaataaatt ttttcaatcg ttccagtatg
    17281 gattgggcta cctgtggttt caggtctctt gccacaccac ccacaatgga gaaatcatac
    17341 tgtatacggt taccacaaac cgcttcgagc agttccattg aaaattcacg gtactgcatg
    17401 gtcttcataa acaacgcttc aaagccggag ttttctgcca catggctcaa gcataataaa
    17461 tgagagtgaa tgcggtccag ttctatcacc agcatcctca gatattttgc ccgcaacgga
    17521 atttcaatct tcagtaattt ttctgccacc tgtgaatagg cggttgtgtg tgatatagaa
    17581 cataatccac atacacgacc cactacaaaa cttacctgcc ggaatttgaa tttggaacag
    17641 caggcttgtt cgataccgcg gtgtacataa tatacattcg cttctgttgc tacaattttt
    17701 tcattctccg tggtgaattt aaaatgcaag ggctccagca gggaaatatg ctgggcgcct
    17761 attggtattt cgtattgcct tcccatagtt atttcttttt gcgtaatggt ccgctttcaa
    17821 aatcaggttc cagtacaaat cctttttgag tgttttcaac ctttatattc atcaggtcca
    17881 ccagctctgc ttctgccacc catgcggatg ccacaatatc tttaatgctt ggtatttctg
    17941 tttcaggact gacgagtgta taaaaacatg ttgtatcgca gggatgtgca taatcactga
    18001 agaaccattg cacttctatc tgatcgccca cttccactgc attcatcacc atgaagtgat
    18061 gcaattcagg tttgtaaaaa tcaccaatgt cctttttaag attctctatt gaagtcatca
    18121 ctttgttcat gacaatgcag gtttaatttg atccgcaata tttctgcggt tgatttggcc
    18181 ttcctctttc aaaggttctt tttggaggcg caccgacact tccatcgctt ttgttttctg
    18241 ttccagtata cccaatgcct gtaccactcc atcaatgatc tgttccggcc tggtaccaca
    18301 tccgcataca tacacatcta ccgggatgta ctggtccacc cctccttcac aattatacat
    18361 tcccctgaac actccgccag tacaggcgca ggcaccgcaa ctgacaacca ctttgggttc
    18421 cggcatttgt gcatagatct ccactaatct ttcccgggaa cgtttggtaa cgggacccgt
    18481 caccagtaaa atatcagact gtttgggatt acctgtattg atcataccaa aacgttcaat
    18541 atcatacttg ggaccgaggc aggccagtat ctcaatatca cagccattac atccgcctgc
    18601 attgtagtgc agtatccagg gtgatttttt tctgtaagaa gccaggttca tgtaaaaatg
    18661 tttattaata aattaataaa agcaagtgaa aaggatacag tcagtgaaaa acgcagcatc
    18721 tgtttataat tcacccttgc agaggaatta tccagtgcat tcaggtaaaa gaaagtgaag
    18781 accatcaatc caattccaag taaaatatta ttccctgcaa acaggaacac cagtgtgtag
    18841 cagaaaacat aatcgagcca tttagctgtg tacaccgctt cgaaaaaaat accggaaaat
    18901 tcaatttcca ctccaccggt tacttcctgg tgtgcttcgg ccacatcaaa tggagatttt
    18961 ttcaatttga tgggcagcac cagcaacaag gcaataaaag ccagtggcat tttatataat
    19021 aaaggctgat cccagttgaa tgcagccgat gcatcaaaac ttccggtaag cataaaaata
    19081 gaaacggcaa taatgatcag caccggctca taagccagta tgctgatagc agtgcggttg
    19141 gcacccaggt ggctgaaaat agaccgggtg ctgtaaccag ccagtataat taaagaagtg
    19201 gacagcaggt gaagaaaaat aaccaggatc aggtcaccac ccagcaacaa tacagctaac
    19261 gcaaaccaca gtgaaacaaa atgcatgatg ccgagaaatg catgggccga gtgaacgatc
    19321 atttttcttt tatccgccag tttcagaaaa tcatagaagg gttgtaataa gggaggacca
    19381 atccttcgct gcattcttgc tttaataatt ctttcaaacc cgtataccag cccgccgatc
    19441 agcggggcca atagtatcag taaaaccggg atcaataaat cgttcataac aaatcagttt
    19501 atatcaatgc cgcaacaatc agtgctacaa aaaacaaaat acccgtggtg gtgaaataag
    19561 gtgtcgccct gtctgttgaa aaatagaagg aagagaagga gtagtttatt ttttccccgc
    19621 acatatattc ttttaccctg tccacatttt taaaccggat aaacagggct gtaataatgg
    19681 taaccggcag caggaataaa gcgatcagca agggcaccat gggtaatttc atagcaccta
    19741 tatgcatatt ccacccgtcc atcacaacag cgacaggcat acctaaagtt tgagaagcta
    19801 caggtacgaa ataaccggtc agcaacagcg gcaagcccag cacactcagt ataattaatc
    19861 ccaacaagac atacatagtg cctgcataca ccagccctgt cttttcgaac ctgatcttat
    19921 catggtcacc ggttcgggca atcaataaac ccattacttt aaaatagagc agggataaaa
    19981 cagcgccgcc gcctgctacc gctacgatca ccagtgcacc caataatttt ttatctgttg
    20041 ccagtgcacc taatgtttca atacttagcc atttaccaat gaaagcgccg aaaggcggca
    20101 gcaataaaga cataaaacca atagtgataa ccagggaagt aaacggacca ctttcaccaa
    20161 gcctgtccat atcggaagat tgtttcaggt gaaacaccct ttccaatatt cctgcattga
    20221 gaaacagtaa acattttgac atgccatgaa aaagaatgag taccagggca gcaacaatag
    20281 taacaggtgt acccaccgct gccatcatga tcatcaatgc cagcagggca atggtggaat
    20341 aagccagaat tcttttgaaa ttatcctgtg ataaggcaga gatagcagct gccacaaata
    20401 caaatcctgt taatgcaatc accaccgtgg ccaccggtgt atcctttaaa gccggcgata
    20461 aacgaagaat aatgaatggc gcaattttca ccatcgtgga agagtgcagt aatgcactta
    20521 ccggtgttgg cgccaccatg gcacccagta accatttgct gaaaggcagc tgtgcccctt
    20581 taattaaagc agccacagac agcaaagcca gaggcagcaa tatccccgat gcggatgcat
    20641 gtgccagcag attgctgaaa gtggcttcac catagccatt gtagataatg aaaaaaattg
    20701 ccgccaatat tgctacccct ccaacctggt tcatccataa agcagtcaat gcattcttaa
    20761 cagacagctc atcttttcta aaaccaatta agaggaagga agccagtgtg gtcagttcaa
    20821 agaacaggaa aaagtattca agattattgg aagaaacgac caggttcatc actgcaataa
    20881 accagaacag gatagaaaga aaatgttttt tacggaaact gctgcaatgt tcttcgtcaa
    20941 tataccggag agaataaatg gcaatgatac cgctgatgac attgatgagg agaaacataa
    21001 atgcactcag tttgtccacc atgaactgca gggaatgatc agcaggtaat attttcagca
    21061 catacaataa tccgccgaat tgcaatacag tcatcaaacc caccagccag cttctccggc
    21121 gaagggctat ccagccaaaa taaaccagta atacaatatc tgccactgcc accaggtcat
    21181 ttacataatg gggaacacca aaatataata caccatccag gttgatgaac acataaagtg
    21241 acagtgctgc taacactaaa gagctggtaa taaccagtat cctcgcaact gttttgggta
    21301 aaatcaataa taagccgctg acaataactg gcaccagaaa taagctagta ataagtggat
    21361 acatgacccg gtattttgct tgtcaaagag gatgctatat taggacgaaa tttacgggct
    21421 gggtatgatg catatcactt ataagtatga tatggcttat gtatacaact aattcttatt
    21481 ctgttagtaa taagggatga agaactatgt gttgtcaagg tgattatgct gttttacgaa
    21541 cagccgggac aaattctttt tcaaaaaatg aaatggcttt ttgcaggttc agctctgcat
    21601 ctttacccag gccttttcca agaccccagt catgtcctgc tatggccatt gtatacgctt
    21661 ccggcgtttt attatacagt tccctggcca ggtacagaat tgtttccggg ctttgcatat
    21721 gcgaggagaa aaaataatgc ggcgctgcca ggcagggctt cacttcaaat ccttctttca
    21781 gttcggtatg tgaggcatct gcaaatatta cggtatcgta cctgcttacc attaccgcat
    21841 cttctatctg caactgataa cggtattcac aatcaataag actgccacag gaacgactga
    21901 ccttgtcagc aaactcccag cctaacccat catccctgcg accactgttt cctatagcga
    21961 tgagcagtat atccgcctta tccgttttta tatttttcat cgatcagctc cccattataa
    22021 ttatacaatt ccgctttcag cggcatttta cccatggcct gtgtggcaca acttaagcaa
    22081 gggtcgtatg cccttattgc tacttctacc tggttcagca taccatcggt aatatgtcct
    22141 ttacggctga tgacattatt agccacccac tttacggccg cattcatcgc atcgttgtta
    22201 tgcgtggtgg atacgataag attgcatttg gtgatcattc ctttttcatc cacttcataa
    22261 tggtgggtaa gtgtgccccg gggtgcttca atgataccaa ttcccttcga ccgcggtgta
    22321 ccttttcgta ccaggtcgtt gcccatcagc tcgggatcgt gcagcagtat tttaatttct
    22381 tcggcacaat gcaacacttc aatcagcctt gcccaatggg aatacatggt actgttattc
    22441 actctctttc cggtaaaagc aatgaactcc tgccttgctt tctctgccag gggagtagta
    22501 atgaaatcac atacattcat tcttgccaac gggcctaccc tgttccagcc attttccctt
    22561 cctacttttt tcaggtaagg aaatttcata taagaccatt gtgccactgc ttctgaaaaa
    22621 taatcctggt acagatcagt ggatatatca tccagtatcc tgtttccttc actatctaca
    22681 gcccgtaaca gcccatcata caaatccaat ccgccctgtt tgttcaccat acccaggtgt
    22741 ccggaaggaa agaaagcaaa atcatcaagg aacttacggt gctgctcatg atagtttttc
    22801 ataaactcca ccatcgcctg agaccattcg atcatggtat caatgttctc aatgttttta
    22861 ccatcaagaa agtactccct ttcattttca gttaatgttt tatgaacacc acccggaaca
    22921 gccagtatgc catggatctt tttaccggca accgctttga tgacttcctg cccgaatttc
    22981 cgcatagtga tgcctttgat ggccagttcc cggttctcca cagctaccgc taccacattt
    23041 cttttttcaa ccggtgcatc cacgccaaat aataaatcgg gagatgccag gtaaaagaaa
    23101 tgtaatgcat gagattgaaa cacctgtcca aaatgcatca ggcggcgaag cttggtggca
    23161 gtgggcgaaa gatccttggg atcaatgcca actatctggt ctatagcttt ggcagcagcc
    23221 aggtgatggc ttaccgggca tatgccacat aatctttgta ccagcaccgg tgcctcccaa
    23281 taaggatggc cctggatgaa acgttcaaag ccccgaaact ctacaatgtg aaaaaaagaa
    23341 tctttcacct ggccgtattc atcgagatgt atagtgaccc ggccgtggcc ttccactctt
    23401 gttacagggt ctattgtaat ttttctgttc atatatcaat gcgttaatcg tatttaaact
    23461 cggaatacaa aatagaatac tcttctcccc acaaaatatt cttcaccgcc ttccagatgt
    23521 gattggcatc gggcgggcag ccgggtatat aataatctat cttaactatt tcattgcagg
    23581 catataccct gtcgaggatc tttggcaggt cttcgtggta aggaatgatg ctggctccct
    23641 gttcgctggt ggcagaattg aggtatgctt cttccaggca ttcactcagc ggaatggagt
    23701 ttctcatgga aggcagtccg ccccaaacag aacattcacc caacgctaca agcacatcac
    23761 aatgttcccg gaacttacgg agtgtttcta cattttctga attgcagaca cctccttcga
    23821 tgataccaat atgacaacgg gaagtaaatt ctttaaaatc cgtcagcggt gatttatcaa
    23881 aggacaccag ttccagcaca tcgagcaggt ctgtatcaat atccagtaat gacatatgac
    23941 aaccgaaaca accggccagt gaaacggtag ctacaatttt tttacccata accggcggcc
    24001 ggttcctgtg tgtttgtttt ttcttgttgt aatccttctg cacagattcc atatcaaact
    24061 tcctgtcgcc aaaaggtgcg gccaggcttg ctccccggac aatgatggcg ccggtcggac
    24121 agatcttcat ggcattgatc gcctgttctt ctgtaagctt tgcttcctgg tcataatcaa
    24181 tccccacata cgtttcatta ccccggtttt gataggtaaa tactttttta ccatcatcgg
    24241 tttttacatc caccacgcac cgcaggcatt tgatacaacg gttatgttcc atcaccatgc
    24301 ggctgggctc gaaatcaaca atacggtctt tgaacaagtg cgggaaacgg gtagaagata
    24361 cacccagctc atagcccatg tgctgcaggt cgcagttacc gcttttttca caagcgggac
    24421 aaatatggtt gccttccgca aacatcattt ccaccagtgc tttacgggta tcaaccagtt
    24481 ccggcgtatt tacttctact accaggcctt cacttacctt ttcagtacag gccgggttgt
    24541 atttgccatt cattttgcag gtgcacaccc ggcaggtacc gagtggcggt tcaatgtgtt
    24601 cgtaataaca aagggatggt atgaagatgc cgttttcttt cgccacctgc atcaggttct
    24661 ttcctttctc tgcctgtatg cttttgccat cgattgttat gttaaccatg tctgccatgt
    24721 catcagattt tatatttctc gtattcttct gttgcgtttt ccatatcaaa cttcaggttc
    24781 agaccattaa aatctttatc cagtttatta tggaagtaat catggaattt ttccatcgaa
    24841 aagatcaggc tggttgtagc tgttttgccc aggccgcaac ggctggtgtt tttcataatg
    24901 gtgccccagc ttttcaattc ttccaggtct ttctcattag ccagcccgtt atccagtctt
    24961 tccagtttac gttgtatgat aaaattgccg gctctgcagg gtgtgcaaat accacatgat
    25021 tctgatttga agaactcaga gaagttgagt aatactttta caagatctct ttgtttgttg
    25081 aagatcataa aagacccccc gcatcgtata tcttttcttg ccagcaggtc aagcatggaa
    25141 atgcgccggt atttttcttt gatggagatg cattcacccg atggtccgct cacctgtata
    25201 taataaggat catcagcttc acagagttcg agtagttctg ccactgtcat gccccattct
    25261 atttcataga tgccgggcag tttgcagtcg ccggacacac tgatgagttt ggtaccgggt
    25321 gagccgggta tgcccatttg catataagcc tctgcaccca tttcaataat gcgggcagct
    25381 gcacagaatg tctccacatt atttataaca gtgggctttt gcaaataccc cttttctacc
    25441 gggaagaacc atttcgtcct cggttcacct cttttacctt ccagtgattc cagcaacgca
    25501 gtttcttcac cgcatacata agagccggca cccatctgta ttctgatatc aaaattgaat
    25561 cctttgatat taccacaatc ggttcccaat aaacctgatt tccggtactc attgatggcc
    25621 tcttccagtt tcggctgcag ccagtgatat tcacctctca ggtaaataat accgtattca
    25681 gcaccgatag tataaccaca gaccattatt ccttcaatca gtgattttgc ctgtgtattc
    25741 aacagcaccc tgtctttgaa agtgcccggt tcgccttcat cggcattgca gaccacatat
    25801 ttcggaacag caggctgatc tttagcggct ttccattttt tccaggtggg aaaataagca
    25861 ccgcctcggc ctgccagttc tgtttttttc aactcttcaa tgatcccgtc cctgcccagt
    25921 ttagctgcat tggcaatagc cgaaccttca gtgaattttt taaagaaaat acttttatca
    25981 ccgggagggg tatagcgtat attatcagcc ggagcatcac aaagagatgc aggcgtagca
    26041 ccatttcgca atgaatgaat gatatctttt actttgatag cattcaggtt ggtaaatgga
    26101 tagaagttga tcagtgctga tggttcctgg tcactgagac caatacaagg tgtaagaaaa
    26161 agtccaaaag tacccgtacg atccacagaa ccaaaagtgg caccggtttc tctttcgaat
    26221 gtttctttta tcctgttgaa ccctttcagg tcggccacaa tgctgttgtt gagatagatg
    26281 gtgtatttac cggttggctt ccggtgaaaa aaatggtaaa aggaaaccac tccttctact
    26341 tcaatatccg acaggttgaa ccggtgtgca agctgcttta tatcatcatc attaatacaa
    26401 ccattgtttc tttgtaattc ccacagctgg tccagcattt gagtctgaat gggttcgttc
    26461 agtgtttttt ctctatcatc aggcatggtt actgatttat tgtttgtgaa aacatcccaa
    26521 tttccaacca tttggtttat aaaactgctg actattgtta agtatatatg tgattttaaa
    26581 cattttggcg aaaacaggca atgcgtaact gtcatcaaat tgtcttttga agtgcagcaa
    26641 tcaggatact gtaatagact caacgcaatt gtactcttac cagtcgtttt agtattgtta
    26701 ttataaccca aaagggattt tcactccgtt taatctccaa aaacatcatt cacatcttta
    26761 tgtgattccc ggaattatta atcctcttat cttcgtttct ctttcaagca aaagaatatg
    26821 aaaatctaac cttgctcagc ctggcatctt actgttaact gagccctttt ctgaccaacc
    26881 gtttatcaat cagcagcatt ccgttgctgt ttatcatttc attttattgt aatttcaaaa
    26941 gattttcaat catgttgcaa cataaaacca acaccaacag gttatcctat tttttttcat
    27001 tggtcaaaca atcgctccgc ggcgatgcac atgactatac acagggcagt atccgcagtg
    27061 ctattgtatt actggctatc ccgatgattc ttgaattgag tcttgaatca gtatttgccg
    27121 tggtcgatat gttttttgta ggcaaattgg gtgcgaatgc aattgctacc gtgggactga
    27181 cagaatctgc aatcactatc gtttactcta tcgctattgg tttaagtact gcggccactg
    27241 ccgtggtggc caggcgaatt ggtgaaaaaa atcctgatgc tgccgcacat gccggtatgc
    27301 agtcaatagt aatagcactg gctgtaacag ttattatcag cattatagga attgtatttg
    27361 ctgccgatat actccgtatc atgggagctt cggaagaagt agttaaagaa ggaacaacct
    27421 ttacccgcat catgctgggc ggcagcgtgg tcattatttt actttttctc atcaatggaa
    27481 tttttcgcgg cgccggtgat gcggccatgg ctatgcgtag cctgtggatc gccagtatca
    27541 ttaatattat cttatgcccg gtgctcatta atggttatgg tccttttccc gaattgggat
    27601 tgaaaggcgc tgctattgca acggtgattg gccggggcac tggtgtattg tatcaatgtt
    27661 atcatttctt caggggtaaa ggtattatca aagtaaaggc tgtacatttt aaatgggata
    27721 caccggtgat caaaacactg gtggaagtag cctggccggc cactttacaa ttcgtcattc
    27781 aatcaggcag ctggatcgta ctggccgtgc tggtggcaaa atatggtggt accgatgcct
    27841 ctgcaggtta ccaggttgct atcagaaatg tggtgttctt tattttaccc gcctggggat
    27901 tgagtaatgc agcagctacc ctggtaggac agaacctcgg tgccaaacaa ccaggccggg
    27961 cagaaaaaag cgtattactg tcgacaaaat acaatgccat ctttatggca ggggtgatgt
    28021 tgctgttcct tttattctcc tcccctatca tcagcatctt caccagcgat ccggaagttc
    28081 atcggtatgg ggttcaggct ttacagatca tcggttgcgg ttatattttt tatggtatcg
    28141 gtatggtgat gatacaggca ctgaatggtg ccggggatac caaaacacct acctggataa
    28201 attttatagg cttctggtta ttccaggtgc ccctggcata cctgctggta aatacatttg
    28261 ggtggggacc ggttggtgca tttattgcta tccctgcagc tgaaacagct attgctattg
    28321 ccgcttatta ttattttaag aaaggaaaat ggaaagaagt gaaagtgtaa ttacaccggg
    28381 cattacaaag taaggaagcg gttgatttga attattgcgg tagttttaca gtaccctgtt
    28441 tccatacttt gtaatactta ctatttattg tggctatgaa taatgtaata gatcagaatc
    28501 gcttacggca aacttttttt atcattctca tccttttgat gggagttgta ttatttattg
    28561 aactgtattc ttttttgcca gccttgctgg gtgccgttac cttatatatt ctcatgcgcc
    28621 gctggatgtt ttacctggca gaaaagaaaa aatggaagaa agggctggct gctttattac
    28681 tgatgttaat ttcccttatc gctattctgg taccggtagg catgctggtc aatatgttgt
    28741 caggtaaagt cacttatgcg gtggagcatt ccactgaact ggtgggtgcc ttaaaaaaag
    28801 tagcttcaga ttttgaacaa cgctttgaca ttgtgatcct gagtgatgcg aacatcaaca
    28861 aactgggtac ctctcttacc caggcactgc caaagatcct gggcgccacc tttaatacac
    28921 tggcagctgt ttttttcatg tatttcattc tctattttat gtttgtcagt ggccgccaga
    28981 tggaaagagt agtttatgaa tacatcccac taaaagacga taatgttact aagctggaga
    29041 aagaggtaaa catgatggtg gtatccaatg ctgtagggat acctgttgtg gctattgcgc
    29101 agggaatagt aggattaata ggatacctta ttataggagt gaaagaaccc ttcttctggt
    29161 ttggtgtaac ctgcattgcg ggtatgttac cggtaattgg cgctgcgctg gcatatatac
    29221 cactcattat tatctttttg gcaaatggac agacatggca gggcattgct atgatatttt
    29281 ttggctttgg cgttatcggc accgtggata atgtattacg attttcactc ctgagaaaaa
    29341 taggtaatgt tcatccgctc actacagtat ttggcgttat tattggcctg aacctctttg
    29401 gttttatcgg actgatcttt ggtcccctat tgatttcaat gtttatgttg ctggtaaaaa
    29461 tttactccag tgagtttatg accaagcaac gggacatcaa taagataatg gagaactgag
    29521 aataattgat tgcactgcat aaacaggctt ctataatcgt attcatccgg gtatagttcc
    29581 ctttctcgta aattttcccg tatagttatt accggtattg tcgtatttgt tcgcaattaa
    29641 ttacaaaaca atgctttatt acttgcatta ttaagtaatc gataatattt tggccttgcc
    29701 ttacgtttga tagtttgaaa aaccgtaccc caatgagaaa actcattctt ccaatatcca
    29761 tgggattatt gtgcacctcg ttcacgatta tcccatctca tcaaactgta aacagtcaca
    29821 ctgtttcagt tactccggat ttatatacag gttcatctta tactactgct gaggcagatg
    29881 cagctactgc attatataat gacatcgacc tgcagcaata tggcttgtca aaagaagctt
    29941 ttgatcatgc ctggaaagga tatcaacatt tagtaaagaa gaaaatggtg agccgtagta
    30001 attatttaac aatctgtgat ttcagccagt catcaaaacg aaaaagacta tacgtaattg
    30061 atattgacaa taggaagctt attaccaaca cttatgtagc acatggaaaa aattcgggtg
    30121 ctgaatatgc caacaacttc tctaataaac ccgaatcgct gcaaagcagt ttaggttttt
    30181 atatcactgc taatacctat accggcaagc atggattgtc gttgcgcatc aatggtgtcg
    30241 atccgggtta taatgataaa gccctgatga gaaccattgt aatacacggc gctgattatg
    30301 tcaacgaaag ccgtgtagtc aatggcggtt atatgggtcg cagctgggga tgtcctgctg
    30361 taccgaaaaa agaagctact gacattatca caaccataaa aaacggaacc tgtcttttca
    30421 tttatcatcc cggcaagaat tatttgcttg gctctaaaat attaaacggc tgattggaca
    30481 caaacaagga tggatgataa caaaaagctc aacttcccct atggttgggc ttttttgttt
    30541 tttaccggca tagacatgta atgacaataa cccccttata gaaattttcc aatgctttgc
    30601 tgtatagtaa tgaataccat ataggtcatt gctactacta ctatccagcc aattacctgt
    30661 aacaataccg ggtgtttgta ttgattaacg acagcagcct tattagctgc aataagcata
    30721 actgctaaag caacaggtaa aataatcccg ttaacagcac cggcgaatac caatagtttc
    30781 actggttgcc cgacatagat aaaaacgata gtggacaaaa taataaaggc actggtgatc
    30841 gttttttccc tgttcctgac aacaggtgat agtgttttcc aaaaagaaac agaagtgtac
    30901 gatgctccca caacagaagt aatggccgca ctccacatca ccataccaaa aaatttatac
    30961 cctatctctc ccgcagctga ttgaaatact gaagcggaag gattatcagc tgataatttt
    31021 atcccctgcc ataccacccc caatgctgct aaaaataaaa tataccgcat aaaggaagtg
    31081 atgaggatac cactgactga gcttttggtt acctgtgaca gatttttttc tcctttgatc
    31141 ccggcatcta tcaaacgatg tgccccggca aaggaaatat aaccacccac tgttcctcct
    31201 accagggtta caattttgat gacatcaatc ttttccggcc atatcgttct gtacaatgct
    31261 gctgcaaccg gaggatggga ggaaaaagca atataaatag ttaacccgat catcagcagg
    31321 ccaagtagct taacaaaaat atctatcagc tttcctgctt ctttatacca aaagattccc
    31381 aacgctatca tacaactgat cattgccccg gtttttgtat caactcctgt cagcacattc
    31441 aggccaaggc cggtaccggc aatattgccg atattaaata tcaatccgcc aaacgcaatc
    31501 agggacgcta aaaaaagacc aagacccggt aaaactttat tggcaatatc ctgggcccgt
    31561 tgttcactta cggtgataat tctccagatg ttaagctgtg caccaatatc gagtaataca
    31621 gaaataagaa taacaaaacc aaaactggta accagttcct gcgtaaacag gcttgtttgt
    31681 gtcaaaaaac cggggccaat tgcagaagta gccatcagga aagcagcacc aagtatagca
    31741 gaattctttt tcatttttga actacaatgg ccagcagaaa accaggccat catagatttt
    31801 actcattatg caatattcat tcttcattgt tgctatggcg aagaaatcag tatgctgttc
    31861 ttttgtaagg tatcatgaat agttttcgca aagtctgctg catgcgttcc atcgccatga
    31921 atacaaatcg tttctgcaac aaccggaatg gttttaccgg atatcgttgt tactgttcct
    31981 tcttttatca tctgtaatac ctgctccatt acttttcccg tatcctcaat caatgcattg
    32041 acctgtgacc ggggggttaa ggaaccatca tcctggtaag tacggtcagc aaaaacttcg
    32101 ttgcttgttt ttaatcctgc agcttccgcc tgcattattg aatgactgcc gctcagacca
    32161 actaaaataa gcctattgtc aaaatctttt actgcccctg caatcacttt cgctaccaat
    32221 gcatcccggg cagatagatt atacaatgca ccatgtggtt ttacatgcac cattttttga
    32281 tcagccgatt cagccacttc actgaaaatg ataagctgtt gggttaccag ttcatacaat
    32341 tcgttaaccg gtaactgcat agttattctt ccaaaattga cccggtcaaa aaaagacgga
    32401 tgtgcaccaa ccgcaacatt gtacttactg cataacttca ttgtatcata catggtttcg
    32461 gcatctcctg catgatagcc acaggcgata ttggcagaac tgatgaaagg cataatgagt
    32521 tcttccctgc caataccttc ccccatatca cagttaatat ctatttgcat gtaaatattc
    32581 ttgcaacctg aaaatacaag cattttggag ttgcagcaaa tgttgctgtt gttttaccag
    32641 caattcttcc gctgtttgat gatcggtcag ccggaaccat atctcatcac ctgttttcat
    32701 ttgggccagc ctggactgat gtgctgttat aacatgggcc acccgggggt aaccgccagt
    32761 ggtctggtga tcagccataa gtacaattag ttttccatca ggcaataatt gtatggtgcc
    32821 aaagccaact gctgaagaaa ccacctcctc atttgtaagg gtaattaggg gagtattccc
    32881 cagcctgtat cccatccggt cagactgttc tgtaataaca aaaggggttg tggtaaatat
    32941 tgttttcgat tcttctgtca gccattccca ttcattaccg ggtaaaaaaa atatttcttg
    33001 tgaattttca tttgctgcaa aaccagcaaa ccagggtaat attttaaaat cattgtcgtg
    33061 cagcaaataa taaatttcat ttttgctttt caataaaagt tcatcgtttt tatacaggtt
    33121 tcttccctgg aagccgcctg caccggcttt aagattcgta ctgctgcttt gtaaccattc
    33181 tttaatttcc aatccaccat gcacggcgat ataagccctt gccccactga ctggtttatt
    33241 aaattgcagt tcatcctttt tattcacagc tatgcaatga tgcaagggca ccggtactcc
    33301 gttaatgctt ggggagaagt cagcaccact taacgcaatc aaagttggtt gagtaaaccg
    33361 gaaaaccgat ccgggaaagt gtaattcgat aaccgcttcc tgtaaatcat taccaacaag
    33421 cgcattggct atttgcatgg catacttatc catcgctccg tttggattaa tccccagctg
    33481 ctggtatccg tagcggccca tatcctgaat ggtatcaagt atcccggctt taataacttt
    33541 cagactcata ctttactcca gtcccctctt ttgaaggaaa gggaattttg aatttcataa
    33601 aaattgttct ctgttattga ataaaactgc actgtatcgc cggcttgaaa tagtgtcgga
    33661 tcttccctgt ctgcatcaaa tagtttaagt ggtgttctcc caatgatctg ccagcccccg
    33721 ggagatctca atgggtatat gcctgtttgt ttcccagcaa tgcccacact ccccggaaaa
    33781 gtaatttgtg gttgtgcctt acgggggatg gcgattcttt catccagttc acccatataa
    33841 gcgaagccgg gcagaaagcc aagcatatat actttatatt ctcttgcggt atggacctga
    33901 atcacttctt cgacagataa atttttaatt gctgccagct gtttaatatc gggagcaaac
    33961 ccattttcat aacaaacagg tatctgcaat aacctggaac tggtttgagg tgctgacatt
    34021 ggcagctgta gtaattcatc cagcttattt ttcatccaat caaatgctgt aatatctatc
    34081 gttgtgtttt ttctgaccgt acatacatca tagcggatgg tcaatgagct ataggccgga
    34141 atcatttcga tcattccgtg taatggataa ttttttaatt gatgaaaccg gccaattact
    34201 tcgttgttga cagcttcatc aattacattt ccaaaatcaa cagtgatcgc tgaatcacct
    34261 agggggaaaa tacggtatgg aatattcagg tagttcatcg gaattgcatt tgaagatacc
    34321 aacttacctt tactccccca tgagccgatt caaccgaaga gacagtttca atttatttac
    34381 caagcttacc ccccgccgtg cctggaatgg tttgaaggtg ttgagcagtt actacatcag
    34441 caaatggaca aaaaaacctg tacaatgggg ttacccgatt tctatttcat ttgaaccaac
    34501 tacttcctgt aacctgcgct gtcccgaatg tcccagcggc ctgcgggcct ttacccgccc
    34561 cactggtatg ttacaaaaag atttctttag cgaaacaatt gaccagttac ataaagaatt
    34621 gctatacctg gtattttatt tccagggaga gccttatctg aatcccggtt tcctggaaat
    34681 ggtacaatac gcatctgcaa aaagaattta tacagccact tccactaatg cccattacct
    34741 gaatgatgaa aacgccaaaa gaacaattga aagtggtttg gaccgtttaa ttatttcaat
    34801 agatggtacc acacaggaag tgtatgagca ataccgtgtg ggagggcaat tgaataaagt
    34861 actggaggga gccaggaata ttgtgaaatg gaaaaaagaa ctgaaaagca gtaaaccatt
    34921 tattgtattt caattcttgg tggtaaagcc taatgaacac cagatagaag aggtaaaaca
    34981 actgggccgg gagatcggcg tagatgatgt ttggtttaaa accgcccagg tatatgacta
    35041 tgagaaagat ccgaaccggc ttattcctac ccttgacaag ttcagtcgct accgcaaaac
    35101 agctactggt tatgaattta agggaaagct ggccaatcat tgctggcggc tctggcatga
    35161 cccggtaata acctgggatg ggctggtggc accctgttgt tttgataaag atgcacagca
    35221 ccaattgggt gatttaaaga aaaaatcgtt taaagagatt tggaagaacg gggaatacag
    35281 taaattcaga caacagatat taaaaggaag gaaaaatatt gatatttgtt ccaattgtag
    35341 tgaaggaact aaggtttgga gcaacgatta atgacaaaag gttcaactat gcaaatacca
    35401 tcacatatat caacccggct tcagtatcag cataaaacac tggtagatct tattgatggt
    35461 ttaaccgaag aacaggtaag aaggcagatc ataccgggca agtggtccat ctttgaaaac
    35521 atagtgcacc tgcaaaccta ccagcatact tttatcaccc gggtacggca actgctcgaa
    35581 caggaaaacc ctgcttttga ccgctatact gcggaagccg acccgttatt tcttgacaac
    35641 tgcagcaaat ctgtccggga aataatgcat gatatgctta ccacccgaaa agaaatagca
    35701 tcaggtatgc tcacgttggc agacacagat tttacaaaaa aagctattca tcctgcattg
    35761 ggagcaatga ccttgctgca atggataaat tttttcctgc tgcatgaagc acatcatctc
    35821 tttactattc tgaaactttc aggagaactg aaaaaagttg acaacccggc cagctagatc
    35881 agctggtata tgtttggttc aacaaacggt acacctcaat aacatttata tcaccaattt
    35941 ctgaattcct tgtaaacata ggtatagaag gaagtaaatc cttcaaaaaa atggcggatg
    36001 gattcggaga gcagcttcat acaataagat ttaatggttg gtggttcgta tagtaaaaga
    36061 actgattttt cagacacaca actatacccc aaagaggtat ttttttgcac cgctgctgct
    36121 ttacaattgt taataaatgg ctaatccggg agatttaaaa gaaattattt gataattcaa
    36181 tgttgcaaca ttaattttgt gctttcaaac actaaaccta aaaaattaat acaatgagtg
    36241 acttaagaac aaatgttgac cagttgaatc aaatgatcct ggaaggcaaa atcctggagg
    36301 ctttcgacaa attctatgca gatgatgtgg taatgcagga caacgactac cctgcccgtt
    36361 caggcaaaga aattaaccgt cagtatgaag aggctttcgt aaacggtctt accgaattcc
    36421 gtggtgccaa agtggtaaac accctcatca gtgatgatct ttcagtagta gagtggtggt
    36481 ttgactatac ccacaaagat tatggtgtaa gaaattatac ccagctggct gttcagcgct
    36541 ggaaaggcgg aaagattgca gaagagaagt tttattacaa taactaagca atacctttga
    36601 gataccacaa caggttttgg gccaggctaa ccgcttcgcc caaaactttt tacatttatg
    36661 aataattgat agtatgtatg ggtattgaaa aagacataca acaggctaaa tttcgtaatg
    36721 cacatcaaaa agcaggcatt aaccttatct atacactggc ctggctgaag gaaaaaacta
    36781 aattgatttt tgaagctgag ggtattacat cacaacaatt caatattctg cgtatcctga
    36841 gaggaagttt tccgcaacca ctttccactt tacagatcag ggagcgaatg ctggaaaaaa
    36901 tgagtgatac cagccgtatt gttgaccggt taatcgccaa aggattagtt aagaaagtca
    36961 cctgcaaaca ggaccgccgc ctggtagatg ttatcattac agacaagggt aaaaaactat
    37021 tggacagatt agatacccgc caggatgaaa tagacggggt attgagcaat ctttcagaaa
    37081 aagatgctaa tattttaagc gacctgctcg ataagatacg ggatgggggt gaatgatgta
    37141 attttgcccg attattcatt ccgaatctga aaacaaacat gaaacgcccc gccacctggc
    37201 tattactgat agcctttttt tctttttata ctaccgaagc ccagcccaag tatgaatttc
    37261 gtggagtatg gatagctact gttaataata ttgactggcc acagtcgggt atgactgatc
    37321 ctgaaaaaca aaaagctgac tatatccgac tgttagatct gcaccagcaa aatggtatga
    37381 atgcaatgat tgtgcaggtc cggcctgccg ctgatgcctt ttatccctca caatatgagc
    37441 cctggagtca atggcttaca ggagtgcagg gcaaaccccc taccccttat tatgatccgc
    37501 tccagttcat gatcgaagaa acccataaac ggggaatgga attccatgcc tggctgaacc
    37561 cataccgggc tgttcatcat attggccagt cttcagttgc tcccaatcat atcagccgtg
    37621 ttcaccccga atggttcctt acgtatggtg ataagaaata tttcaacccg ggaaataaag
    37681 aagcacaggc ttttgtagta aaagtagttc gggacattgt aaaacggtat gatgtagatg
    37741 ccatccatat ggatgactac ttctatcctt atcgtattcc gggtaaagaa tttcctgatg
    37801 ctgcatccta tgcaaaatcc ggaagtaaac tttctaaaga tgactggcgt agaagtaatg
    37861 ttgatacaat tatcctggcg ttgagcaaag cgatcaaaga agagaaaaaa atgtgccgct
    37921 ttggtatttc ccccttcagc gtatggagaa ataaagataa agatcctgtt cggggaagcg
    37981 atacccgtgc cgggcaaacg aattatgatg acctgtatgc tgatatttta ttgtggctgc
    38041 aaaaaggatg gatcgattat gtaaccccgc agctctacct ggagatcggt catgataaaa
    38101 ttgactatgc aaaaatgctt gactggtgga gccgcaatag ttttggcaga catatatata
    38161 taggtcatgg tatctatcgc accgcagaga atcagcctaa atggaaaaac ccggccgaac
    38221 ttcccaacca gataaaacta ctgcgccggt acccgaatgt gcagggcagt atctatttca
    38281 gcagcaaatc atttgaacgg aatccgaatg gctggaatga cagtctccgg gataattatt
    38341 acaaataccc ggccctgctg ccgcccatgc gctggatcga taatgaaaaa ccatggccac
    38401 ccattgtaga aagaagcatt acaaatgatt ctatcataca attggatgtg agaccagacc
    38461 ccagaaacag ggtggagatc aaatattatg ttgtatatca gtatgcggct gattcacaca
    38521 atgaaacttt tggtaatgtg ccccggtacc tgtcaaagtt tgtaaccatg cctgcggggt
    38581 ttcaattaaa ggaacccctt tcgtccaaac atttttcata ccggtatgtg atcacagccg
    38641 tgggaagaaa taataatgag agtgagttga gtgagattgc gatactggta caaccaggcg
    38701 gtagtaaatg gagcttttat aaccccaagc cttaaaacga agcatactaa cgttttttta
    38761 cctttgccag acctgagaaa acaacttcaa acctcaaact acaaactaaa aaatatgccc
    38821 ggttacgaac tctggagtga taaagaaaga aaagaagtaa acgatgtgct ggaaacaggc
    38881 atcctgatgc gctatggatt tgacggaccc cgaaaaggag tctggaagag taaagaatta
    38941 gaagctgctg ttaacgaaac ctttggcagt aaatatgcgc agcttacttc cagcggtact
    39001 gcagctttaa ctacggctat gagtgcattg ggcattggat atggtgatga agtgataaca
    39061 ccttctttta cgtttgtggc aagttttgaa gcgatactaa gtgttggtgc tgttcctgta
    39121 ttggtggatg tagatgatac attgacattg aatcctgaag cagtacgaaa agccatcact
    39181 ccaaaaacaa aagccgtaat gccggtacat atgtgtggca gtatggccga tatggatgcc
    39241 ttaatagcca tatgcaaaga acataatttg attttgctgg aagatgcctg ccagagcatt
    39301 ggcggtactt acaaaggaaa acatctcggc actattggtg atgccggtac tttctctttt
    39361 gattttgtaa aaacgatgac ctgtgccgaa ggtggtgtgg tgatgaccag ccgggaagat
    39421 atttatataa aaagtgatgg ctatacggat catggacacg atcacaaagg ggtggaccgt
    39481 ggtgctgacc tgcatccctt tatcggttac aattatcgta tctccgaatt gcatgctgcc
    39541 gttggcctgg cacagatcaa aaggctgaat gaatttctga ccattcagaa aaagaaccat
    39601 acccagctaa aaaacatcct gtcacaggtg cctgagattt ctttccgtcg tataccagat
    39661 cctgctggtg atagttgtag ttttatcagt tggttcctgc ccagcgaaga aattacaaga
    39721 tctgttgtgg cagaactaaa agcacaggga atattagccg gtaacttcta ctggttcgat
    39781 aataactggc actatatccg caaatgggat catttgaaga atgctattac attaaattca
    39841 ttgagcccgg aattgaaaga acgggtgacg caacaggcca ataaagattt tacagccagt
    39901 gatgctgtga tgagccgttg tatttctact tctatcagtt tactgtggac agaagagcag
    39961 atcaaagaga aaggagagaa aatagtgaat acggttagaa tggtattaca gaacagggct
    40021 gttacaattt gagagcttaa tttcttttta caacactggc aatctccatc aggcatttca
    40081 gtaaggagat ccgaaggttt ttaaaatcgc tgctgtttgt ttgaatagct acacacatgt
    40141 tatgcctggg gaagtataac atctctgtca tgtagccagg aaaaaaaccg ctatgcccgt
    40201 atgcaacccc cagtgatgtt ggccgaataa ttacacccag tccatatttc acatcacggc
    40261 ccaatttagc cggaacagca gctgaaagca acaatgttgt atccacaatt tgtccttcgt
    40321 ataactgctt tccccatttg gctagatcct ccgtagtgga ataaatacct cctcctgtcc
    40381 attcaaactg tgggttcacg ataaataatc catcctcccc gattacttta tcctttccgc
    40441 caaattcgtt attagtaccg gcataaccct gtaccagtct tttcaatttc ctgttatcag
    40501 aaggaaaagt atggagcagt gttagtggta caagaatatt atctctcaat aaatcataat
    40561 atttttctcc ggcaacctgt tctattatca ttcctagcag gatataattg gtatcagaat
    40621 aatcccatcc ttcaccggcc ttaaaaggtg ctttttcatc cagaatataa tggagtaaat
    40681 cttcgggtgc ccatacttta tccgggaaaa aggtaaggtc tttcgtaaat tgttctttaa
    40741 actcataccg catgacccca cttgtatgat tcattaacat gcggactgta atatccgttg
    40801 cattaggtat cctgtaatac cacgcatagt gaccgagata ggttgaaact tttttatcca
    40861 ggtttatttt tccattttta accaattgca gcgccattgc cgagacataa gtttttccaa
    40921 cacttccctg catcaggcaa tcaccaggtt gaaggaggat atttttctca atatccgcat
    40981 agcccgacac cgctgcaaat gaattattgg tcccgtagta aacagcagcc gacaatcccg
    41041 gaaaattccc ggcaattctt agtgaatcaa gcttcttttt gaaatcaact gcaaattctg
    41101 cttgagaaaa agcatcgtat acagttcctg atacgattaa aaataacaat agatagtgtc
    41161 gtaccttttt catttacctg ccaatagatt tttgtcctct ggatttaata ataaaagaca
    41221 gttcgaggaa acttataaac ccggtttaac aacattgggt ttttgctcca tccaattcac
    41281 atatgctaaa gatcccaatc taaaacggag aggaatagca attactttat ccagctgtaa
    41341 ttcttttttg cagaaaaatc ccaggctgtt aagataaaaa gaagtggttg gtaagtttga
    41401 cagtgtaaac cctgctcttc cgctgtattg attgtaatgc cggagttgaa tacccgaaaa
    41461 tataggtttg aataagggca cagccctatg atcaaaagta cttttgacag ccaacagagg
    41521 ttcctgaacc ccgggctttt cctgcgcaaa aacacctttt aaaaacatta acagcaacac
    41581 aacaagaaag ctggcggttc gttgcaccat acttatttaa tttgtgtcta aatttacggc
    41641 attatttttg tgcaaacggc gaatttttaa aaaaataggc ccgccactat aaaaaactga
    41701 acgaaccatg gcccttagcc agagcttaca acaaaggctg cttcaaaagc tatcacccca
    41761 gcagattcaa ctgatgaaac tgctgcaggt acctacggcc aacctggaaa cccgcatcaa
    41821 agaggaaatg gaagaaaatc ccgccctgga gctggatgaa gacaaacatg aagatgctga
    41881 tgatattaaa gatgagttca gtgatacggc tgaagaggac tatgaagaag cagacggaag
    41941 caatgatgaa tacgataata tagatgtgag tgaatatgtg catgatggtg atgatgaagt
    42001 gggtgattac aagcttcgtg acgataacta cccggaagaa gatgaaggaa gacaattgcc
    42061 ttatagaaca gaagccagtt tttacgaaac actgatcgac caactcggcc tgctgaagct
    42121 ggaagaaaaa gaacaagcca ttgcagaaca aattgtcggc agcattgatg aagacggtta
    42181 tttacgaaga gagacagctg ccattgtgga tgacctcgct tttcgccaga acattattgc
    42241 tacggaagta gaagtagaat ctatcatcaa acgcatccag cagtttgaac cgccgggtgt
    42301 agcagccagg gatttgaggg aatgtttact tattcagctg aagcggcaaa aagatgaagg
    42361 tgcacaggta gatatggcca tcaaagccat ttccaagtac tttgatgagt ttaccaaaaa
    42421 acactatgaa aaaatacaaa ggggattgaa tattgatgat gaccagttgc gggaagtgat
    42481 gcagcagatc atccgcctca gcccaaagcc gggaggtaat gttggcggga tcaataaagc
    42541 tgaaaactat gtagtacctg attttttcat cctgaataac agcggaaaat tagagctgac
    42601 actaaacagc cgtaatgcac cggagttacg catcagtgaa ggctaccggg atatgttaaa
    42661 agactatgac cggggcgcca agaaagataa acgtcagaaa gaagcagtgt tgttcatcaa
    42721 acagaaaata gattccgcca aatggttcat tgatgccatc aagcaacggc aacatacatt
    42781 gctaagtacc atgaatgcta tcatggaaca ccagtatgaa ttttttctta cgggtgatga
    42841 gacagaactg aaaccaatga tcctgaaaga tattgccgag aaaacgaacc tggatatttc
    42901 aacagtgagc cgggtggcca acagcaagtt tgttcaaaca gaattcggca cttaccggtt
    42961 gaagtttttc ttcagcgaat ccttaagcac cgacagtggt gaagaagtat ctaccaggga
    43021 agtaaagaaa attctcagca attttataga agaagaagat aaaaagcggc cattgagtga
    43081 tgaacgactc actgaaatgt tacaggaaaa aggatacaat attgcccgcc gtacagtggc
    43141 caaatacagg gaacagttaa atattccggt tgcaaggtta agaaaagagt tataatactt
    43201 ttttcacttg aagcttaaaa aggaagatcg tctttattct ccatctgtcc ctcatcaaaa
    43261 tatacctggt catcctgggc tttatcaatc ttggcagctc cggccgcttc taccttccag
    43321 gcttttacat cggtgtacca gcgtccattg tattcacggc tttctacatc aaaagaaata
    43381 ctcagcatgg agccaacctg caacaggctt tcacttattt tatcgcccca gatagagata
    43441 caaacttttt tggggtactg tccctctgtt tctacaataa tatcctgctt tttccaggga
    43501 ccgtttttcc cggcacctgt ctggaggggc agcaactgta tgagtttagc ggttaattgc
    43561 attcgtcgaa gttatttaaa gaatgaagtt acgtacaggc atttgaataa ttacgataag
    43621 catacccttc agcgggtatt ttgaaaaaaa atcatttatt cgttataaaa tcagtatcca
    43681 aaactgtatt ttacacagct aattattccc caacccactg aacatggttt tcgcaagcct
    43741 tatcttcctg tttctttttc tgccgctgaa cctgctgctc tattacagta ctaagaatgc
    43801 cacctggagg aatagtatcc tgatcctttt ttctttattc ttttatggat ggggagagcc
    43861 tatctggatc acactcatga ttttctccgc attggtagac tatgtgaacg gaagggtaat
    43921 tgaaaaatac agggggacaa tcggggccaa aataggactg gtctcttcct taatcatcaa
    43981 cctgggcctg ctggctactt tcaaatacag cagttttata gttgaaaatg taaaccagct
    44041 ttttggcacc tccttcaatt tgcctcattt tgcgttgccc atcgggatct ctttttatac
    44101 atttcaaact atttcctatt ctgtagatgt ataccgcaat gaagtaaagg cgcaaaaatc
    44161 atttttaaaa ttcctgatgt tcgtttcgct gtaccaccag ctggtggcag gacccattgt
    44221 tcgttacagt catattgcag aggaaataga taaccggaaa gaaaaactgg ctgatatcag
    44281 caaaggcatt tcaagatttt gtattggcct ttttaaaaaa gtagtgattg ccaatgtagc
    44341 agctgaactg gtggcaaaat atatggatgc agatgcttcc tccctggcgg ttggtggtgc
    44401 ctggcttggt ttgatcatgt ttgctatcca gatctatttt gacttttccg gttactcaga
    44461 tatggccata ggtttaggct ggatgtttgg ttttcattac catgagaact ttaaatatcc
    44521 atatactgcc acctctgttt cagatttctg gcgacgatgg catatttcac tcggaacatt
    44581 ctttaaagac tatgtgtaca taccacttgg tggcaataaa aaacatattt acctgaacct
    44641 gttcattgtc tggttcctta ccggtatgtg gcatggcgcc agctggaatt tcattttctg
    44701 ggggctatat ttctttgttt ttattgcatt ggaaaaagca ttcctgttga agacacttaa
    44761 aaaactaccg aaattctttt cccatttcta tgcattactt gtcattattc cgggctgggt
    44821 aatcttctat tttacggata caaataaatt atatgcctat atagaaagat tattttcatt
    44881 tggttccggt aatctctgga atgtcgggtt aaccagtgat cttacggcac atttgttctg
    44941 gtttattttt gccatcattg cctgtatgcc ggtttatcat aaagcaaaag cattttttga
    45001 atcgagtctt aaaaaaccag caatttttga aacagcggcg attgcagtaa atattgtatg
    45061 tatcttttta tgtgtggcac aattagtggg taaaagctat aacccgttta tttatttccg
    45121 gttttaaccc ggataaacat attttacagt gagcatgaca aaaacagaaa acactattaa
    45181 tattattaac gtaaccgttt ttacgttagt gcttggtatt ggtgggttgg cttcgctttc
    45241 catgaaaaaa caagcagtat cggaaataga aaatcgtcaa ctaaccccct ttcctgctta
    45301 ttctgacagt gctttgtgga atggcaatta cttcaggcag gtagatcaat actatgccga
    45361 taactttccc ctgagaaacc ggtggatcaa tgtatcaggt tcattccgca ataaatttgg
    45421 ctttgaatca ggtgacatta aaatatatga acctgctaat gatgcagaag ccaatgaaat
    45481 aacagatacc acaaaaaata cggtcaactc aggccctctg ccggacgatg gtgccgtggg
    45541 tgaagtaaag aaaagattat ttgtgtttaa gaacagggct tttgaaatgt ttggcggtag
    45601 cactgcgatg ggaaagtctt atgccaatgt gatcaattct tataacaggc tgggcattcc
    45661 tggtctgcag gtttacaatt taataatccc tgtggcattg gagtttgaac tcacagaaaa
    45721 atataaaaag ctgcaaaaac caaaccggcc ggcgatagaa agtatttaca atgaactgga
    45781 ttcaactatt aaaaaggttt gggccattga cgaaataaga aaacaccggg aggaatatat
    45841 ctatttcaat acagaccacc attggaccag ccttggtgca tattatgctt accgtgcatt
    45901 ttgtgaaaca gcaggattaa cgcctgtatc actggatact gtccgataca aagtaaagtc
    45961 caccttcctc ggttctttat acaggctgac cagagatccg aaacttaaag aaaatcctga
    46021 ctcggttaaa tattatctgt tccgggactc cgtcaggttt tacataggca gcagtaatag
    46081 ccttacgtat tggggcaaat caaaaatgta tggggagggt gccaacggat ccaatagtta
    46141 ttctgttttt ctgcagggag atctgccaat cgtaaaaatg gaaacacaac ataataatgg
    46201 aagaaaaatt gctatagtaa aagaatcgta tggaaatgca tttgctccgt tcctggtgaa
    46261 caattatgaa aaaataattg tggtggacca gcgttactat acgggtgatt ttgttgcaat
    46321 gttaaaaaaa gaaggtatca atgagttatt gttcatcaat aacatcttcg ctgcccatac
    46381 accctttcat attgaaaaga taaagagatt aaaaaaatag aaataagaac aaggtccggg
    46441 ctttaaaagc ctttctactg aacctgctct tcaaatacca tagcgtagca cactcctatt
    46501 tttgttatag ctgtaaatgc tttcgcaccc aggccacttt aggttttaac tcaccggtat
    46561 atttatctcc ggtagcatat ttcacatgtt tcaggaaatg ataataatct gccttgggat
    46621 gttttgcccg ccatttatcg tagcgtttgt cctgccatgt tttatagtaa gcaatacatt
    46681 cttcatcaga gttaaatttt ttgcatttat tgcccttgat gtagaaacca aacaggttgt
    46741 tatagcggag gcaacatttt ttgcaattga ggttacctgt ttcctgcata cattgagcca
    46801 taacaaaatc gggatgccgg atacctgcgg cctttatagc atcataggta gcctgcattt
    46861 tggctttatc tattttctgt gaattcccct ggtgaaatgc caaaaaaagc acaatggcaa
    46921 aaagtaattt cttcatactg aggtttttgt aagcaatacg gttttcaacg ggttagcaga
    46981 ttgataataa atatacaaaa atcgcaccct ttttatcaat tgctttactt tgcacaaata
    47041 gcgaattatc aattatgaat acagtcctga tgatgaacag caagaatcag ttgcataaaa
    47101 caaaagaaca gggtgccgga accccggcca tcatcaggtt gctggcatca tttttttctt
    47161 atctttttca cccggtattt atccctttat atattgtggg attcatggtg tatgaacatc
    47221 cttacctgtt tgcaggattt gcaccaaaag aaaagatact ggtagtggca caagctttcg
    47281 ccatgtttac attttttccg ctggttactg tattactatt aaaagcgctg aagttcattg
    47341 aatcatttca cctggctacg caaaaagacc ggatcatccc attagtagct tgcggggttt
    47401 ggtatttctg gatctggtat gtatggagaa accttccgga ttatccaaaa cctgctgtgc
    47461 aattagcaat ggctgtatgg atcactgcca gcctcgggtt aatggcgaat atttatatga
    47521 agataagttt gcatgctatg tctcttgggg tgatggttgc ctttatttta ctactggcat
    47581 tttcgcaacc actcaatttt ggtatttata tatctgtcag cctgctgata gcaggaattg
    47641 tgtgtacatc ccggtttatt gtatcagatc attcagcaaa agaactatat ggtggtttac
    47701 tggtgggagt tatttccatg ttgattgcca gctggttggg atgatataat ttataatttt
    47761 gtttaccaaa cattccagtc tacggcaatt gtttcattcc gggaggtagg tttttgcaat
    47821 ccctttgtca gctcttccac tctttttcca acaatatttt tcagctcgct gatcttcatc
    47881 gaaggatgac tactcattgc ctccattatt gaataagtga ataccccgtt tttcagatca
    47941 tttctctcca gggcaaactg tgttccggct gctgcagaaa taatcgtagc acctgtactc
    48001 ttgcctacat tcacaaacaa gctctgcatt aattcaaaac tatttctgag tcccaggtgt
    48061 ttttcatcct ttttataagc taccggttta aatcccttta ttaatgaatc ggatgtagtt
    48121 gccagtgtaa ccaggtcttc cttatccact tcaccactat ggcaggcatc tatcagcatc
    48181 agttttttcc tggctggaat actgtctaac aggttttcca gttcatcata aggcaaaccg
    48241 ttttgttctg gcatttcaaa attcacagca taagttgaaa gatagtagtc gtactcttta
    48301 ctcagcatac cgtgaccgga gtaagcaact atcactttat cattttctgt tgtttgcagc
    48361 agtttttgtt ttaatgcttt tacagtattg atggtaacat tctcgttgaa taatgtatca
    48421 ataataatat tgcctttgta tttttctttc aattttttgg acaggtcacg gatgtctttc
    48481 acactatatt gtaaattgta tttactttca gaaaatttat cgataccaat cccgataaaa
    48541 cgaactgatt ctttttgttt taccgccggt aaataattta cagtaagtgg catacgataa
    48601 ctttcagtac cattcacatt actgattgaa gtttcaatcc tgttttctcc ctgtgacaat
    48661 ttaatagtga tcgtagtatc aaaacttaag gaattccgcc taagaagact gattcctttt
    48721 agcccaaata ccggcacttc attcacccat acattaaagc ggccaagcag gtaagtgctg
    48781 tctctccctt ttatatgaag cattagtttt tcctgatttt gttcggattc aatagcatcc
    48841 cggttgacaa agtcacattc aggtacacta taaccatccc tgaaataagc tgtatctact
    48901 cctaattttt tcagccgctt ctggtacgct ttacggtagg cattgatcaa tgctttatca
    48961 ttactcccta ttgtttcgag cactttatcc ggacggttgt atttaatatc cagttgttca
    49021 aaagtgatga ctttcagatc ttttgttaca taatgcagca gcttcgtggc tccgggcgaa
    49081 gcctggtaat aaccggcagg cacctgtgta aaatactccg tactgtccac cgggaaaaaa
    49141 tgatacaata ttttctttgt ctttaaatca accatcttga caaggctggc agtggtagac
    49201 aacagcattc ttttgttgtc gggtgcaaat gcagctgccc ttacataatt tccaggctcc
    49261 ctcagcgacc atatctcttt tttgctatga atatcataaa ccttgaatgc agcccatccg
    49321 tcattaaaac aaatcatact gttatcaggg ctaatctcgc cacctataat atcattaata
    49381 ccatcaccgg tgcccgttgt aatagtagag accagtatat ttttcccgat atcaaatatt
    49441 gagatatggc catctgcacc aaagattgct gccagcgttt tatcattact aaaacagccg
    49501 tgggtaatcc tttcctcttt attatacggc agctttgtta ctattttacc ggttgccata
    49561 tcccagataa ttgctggctc cctgtcatta tacatggttg tcattaaaag tgtattgtcc
    49621 cgtgagaaaa aagcattttg aattttttgc ggcaactttt tattccccct gaattgacta
    49681 agcctgctgc cctttttgtt accccacaca ttaaaatcat tgatactgtt gacatcctgc
    49741 actaccactg aataactccg gtcccaatta aagatgatat tcttattagc atcaccccgg
    49801 tgtacattgg gacgggtaat tcctttatag gctattgttt tctttaattg catagtgttg
    49861 gcagcccagg cagtagcaaa taacctgtca tctggttcca tataaatggt atcccctttc
    49921 ggcgaataat aaagcagcct ggtcatatat gtatgaccat tgagggtggt aaatattttt
    49981 ttacccaggc tgtcagtcac caatacatcg ttcctgtaaa tatcgtacac caattttctg
    50041 ccgggattgg gcagtaccgg tgtaaaatcc cactgaccgg attccccgga aagaaaactg
    50101 gtatcttttg tggtaagttt tgtgatcaca aacctgtcaa ggctatcatt ataaaccgtt
    50161 tttgctaata acataccggt ctttgtattc cagtaatgcc tgccgggata agatattgtt
    50221 cttacaatat ctccattctg attaaactgc accgaactga cctctgattc atgttcgcct
    50281 ttaccaagta caagtagcag tttacctgtt gagatatccc aaattctggc agacttatcg
    50341 cctgatcctg atataaaaag agtaccctgc ttattaaatg ctccgctggt aataccatct
    50401 tttattactc ccaattttac aagtggtaat gctttttttg catcccatat aatcaaagta
    50461 ccatcccggg aaccagtaaa taacattgta ccatccctgt tgtagccaat acaacttatc
    50521 ctgtctgcat gccccggaag attggctaat aaataaccgg tccttgtatc ccaaatgcgg
    50581 ggaacttcga agcgatcacg ggtaactata tgtttatagt cagggctgaa aattgcttca
    50641 cgtacaccac caatatgacc gacaggtaat attagttgcg gctcctgtga accagcgtaa
    50701 acaggaaccg ataaactaat taccagcagt aaagctggtt caaaaaaaat atggatgaat
    50761 tttcctttac tcattagtaa cctatctatt acagaatatg ccattcccgg gcaataattt
    50821 taatgccgga tgctggtttc agctaacctt ttcaccaact cctgccttat cagaaaaata
    50881 ctgctattct tttatgtcgc caataccaga taccgggtat gcctatcact ttccggtaat
    50941 ttttaaatta ttccccaaaa tattagtttt gaattactaa atcatttagt tttgaatcct
    51001 ggtcgggatt ttccgactta caatacgcaa atacataact ttaacccatg gcaaaaacag
    51061 aaaaaactac agacatgtca accactaaca gcgaaaaatt aaaagccctc aagcttacga
    51121 tggataagct ggagaaggac tacggcaaag gcagtgtaat gatgctgggc gataaagcag
    51181 aaaccatgca ggaagtaatt tccaccggtt caatcgggct ggatgttgcc cttggtatcg
    51241 gcggcttacc aagaggaaga gtcgtagaga tctacggacc tgaatcatcc ggtaaaacca
    51301 ctatcgctac ccatgtaata gccgaagcac agaaaaaagg cggcatgtgt gcctttatcg
    51361 atgctgaaca tgcttttgac agttcttatg cgaagaaact aggagttgat gttgacaacc
    51421 ttttaatatc acaacctgat tatggcgagc aggcccttga aatagccgac cgtcttattc
    51481 tttccggggc attggatgta gtggtaattg actctgtggc agcactcgtt cctaaaagtg
    51541 aactggaagg cgaaatggga gacagtaaaa tgggattgca tgcaaggctg atgagccagg
    51601 cactacgcaa actgaccgct actatttcaa aaacaaatac catttgcatc tttatcaacc
    51661 agttaaggga aaaaataggg gtgatgtttg gtaaccccga aacaactacc ggcggtaatg
    51721 ctctgaaatt ctatgcatcc gttcgtctcg atatccgccg catgacacag gtaaaagatg
    51781 gtgatgaggc cattggtaac catgtaaaag taaaagtcgt aaagaataaa gtggcccccc
    51841 cgttccgggc tgccgagttt gatatcattt tcggggaagg catttccaaa accggtgaga
    51901 tcattgatat gggtactgaa cttgggatta ttcaaaaaag cggatcctgg tatagttata
    51961 acagtgataa attgggccag ggccgtgatt ccgtaaaaca attaatggta gacaatccag
    52021 aactggctaa agaaattgaa gggaagatca gggagaaaat aaaagaaatg cagacagcat
    52081 aattgactta tatacaatgt attaacgatg ggttagtgta ctgaaccgtt ccgcctccgg
    52141 cagaacggtt ttttatttct tcacaaatat tttacaagaa aaaaaacaaa tcaatgttta
    52201 aacattgtat atttgttctg tcctccccgc aaccaattaa agcagggaac cgttaataca
    52261 gtttatttat atgactacca catcacctaa aaccagttta ttacaatcgc tccatttact
    52321 tttcggagcc ggattgctgg tttccttttt ccttccctgg gtggcctgga aagatattaa
    52381 agtgagcggt tatcatatgc cagccggaaa ttttttcaaa ttatcggaat ctaattttgg
    52441 attaggtaat ccatttcccc agtttgattt tactttttat ttcttctggc tgatccccgt
    52501 cttagttgtt gccggaatcg ttattgtttt acaaaataaa aaaacttcca tccctgcttt
    52561 tctttccggg gcattggccc tctctcttgt cactgtttac tatttttttt cgaatacact
    52621 ggtcgacctg ggagtaggca acagcgcatt tgatatgctg gaaccgggtg cttttattac
    52681 cgtttttact gcaataggtt ttattttgac tacacttccc ctgcatagtt ggtttaaaaa
    52741 aatggcctgg attattatag gccctttgtt agcatgggga gcatataaaa ccggagagca
    52801 atctgtactg tctgaaactt ttacccatac tgaccaggta gcagcagatt atactgctga
    52861 tgctgcgggg ctgataaaag aatttttgac aaacgatacg gccgctaaca aaaaatacct
    52921 ggagaaaacc attattgtaa atggtaacac ttccgctatt gaaatactgg gtgactctac
    52981 aagcaccatc aaatttgaag acagtaccgg ttcttatgcc atcttctccc tggaaaaaag
    53041 tcagtttgat aaaataaata agattaccag gggagaggca gtttcactga aaggtgtttg
    53101 cagtggcagt atttatagtg aaatattggg tacaacagcg ataacattca aaagagcaac
    53161 ttttaatatc aaataaatac aattaatcaa caaatgaaaa aaacaacact tgttctggct
    53221 ttagtattcg ctgcaggcgc agcgtttgca caaaaaaaaa ctacatcatc agcaaccgtt
    53281 tcttttgatg ccactacacc aaaagatgcc ttacccaagg cagaaaacaa aacggttatc
    53341 ggttctattg atactcaaac aggtgccgtg gcttttgaag cagcagtaaa aaatttcgct
    53401 ttttccaatc ctaaaatcca ggagcatttc aacgccccca actggatgag ttctgaccag
    53461 tttcccaaat tcacctttac gggaacgatc gataaagtaa ataaagtaaa attcgataaa
    53521 aacggaacct atactgtaaa agtaaacggt aacctcaccg ttaaaggaat cagtaaacct
    53581 tcaaaggttc aaggaactgt aacagttgct gatggaaaaa tcaaggttgc agctaatttc
    53641 aaaatcaaat tagaagatta taatatcacc ggtcagccga ttgaagccgg caaaatagat
    53701 aaagaaccaa agattacagt ttcagctgag ttctgataaa aatactccta taaaaatgaa
    53761 acgccccgct ctattgtggg gtgtttcatt tactgagctt tatttagttc cctatctctc
    53821 tttccaggtg tatcagcttt ccagttgcat caaacccttc cacaatcact ttgaacttct
    53881 tgctgaaatc attattataa aaatttaatt taaccgtcct ggtttccata tctgtaaaca
    53941 ggttggaatt ccagtatagc gttgtccttc catccacccc gatctgcttt gcttctgctg
    54001 tagtatagga aggactataa aattcttttg tgattgaata tccatttgct tcaaaaaaac
    54061 tcagcttatc cggcttttgt tcatccataa atttttcttt agtataaata gacagcgccc
    54121 cgcctggtga accgctaccc acccctacaa aaccggcttc ataaaactta accagtgcca
    54181 catcggttac tctaattgtc cgtaaaaaac ccatatctgc aggttgttca ttcaggaaaa
    54241 gtccaattac ccatttctgg ccactcatca aactcatatt tttcctgttg acaaagcgtc
    54301 cgccctgtat ttccacctgc tgaatcctgt tcttaatata atcaatcaca ttcattgatt
    54361 tatcgtttgc cggctcatta atattatcaa ggttcacttt gccggctgtc ctgaatacac
    54421 cagtggtata tttctcattc acaatatcaa aaggcttttt agaggatttt gattttatat
    54481 caacattttc caactcctta attttttcct caaagccttg tgtgtatgta tagcgcctgg
    54541 ctatctcttc attgtaaggc aagccggata gattcttctc tccaacttct gatggaatca
    54601 acaaaggaat ttttatcacc tgcttttcca tttcgttctc atcggtaatt gcaagggcag
    54661 gtttgggttt accctgttta tctgtataag cataataaaa ttttgcttta cccatgaaca
    54721 ctaatgagtc cagtatgaat cttccctgtt cggaaaggat cccttcctgg ttttgagtag
    54781 tggagtcctc cgcttcaata tagatattaa gtttacctgt tgttaatgga gcattggttt
    54841 tctcatccac aactttaccg gctattctaa taaggctttc atcattaaat ttcttttcag
    54901 gaaactcctt tgccaatatt ttattccagt taaaccgtct ccagccatgt gtaagcatga
    54961 ggttgtccat agccaggtca gttgaatcag ttttactgtt aaaataccag gcaggattat
    55021 gtatatatcc tttcagatcg tctgtcaata aaaaccggga gtaaatattc tccttatctg
    55081 taagaccacc acccggaata tcaacgactg atacagaaaa acttctttgc accatatcgg
    55141 gaaaactcag ttcaaaaata ttttcttccc gtttaccggt acccagtttt actatattga
    55201 tatccggttt caccttatat tcttcattat tgacaaaaga aagccgttca gcaagaggaa
    55261 cacctttatc actaaaaaca gtaaaatgta agataccgga aggcaggcta tccgtaatga
    55321 ggtgaccaat gattgaaggg tacccctcaa aggcgatctc tgtttcatac accacatgat
    55381 tatttatttg cgctaccagc aataaatttt caaattgctc cttttctttt ggccccctta
    55441 ccaattgaaa ttttttcccc cctttttcat cctgaacttt caggattatc cctgatgccc
    55501 ggacttccgg aagattgaac tgtcttttcc cagctgcagt ctctgtttcc gcaatatatt
    55561 tcttccctgc cagtggttta aatggcaccc tgccaatacc atcatgatag gttttaaacg
    55621 gggcaacagt aattccgtct tcggttttta tcattccact tatctcaacc gggatgccga
    55681 attggtcaac tgctttaaaa gcaaccattg tcagtatccc atcaacgagg tatccgctct
    55741 caggaaaaaa ctggagggag actgtttgtg ataattttgc agctgcaggt gttacccctg
    55801 gtttaaaaat gacaatattt tttttgtaga caaacgactc atcattattc agcatagccg
    55861 gtgtcaatgc ccggatataa taattgccct gtggaagaga atccggaata ctgatatttc
    55921 ccctggcagt agcccctaca accgggtatt ttcctgaact gataatattt cctttagcat
    55981 ctatgaactg caggtaaaaa ttattactga cagcatccgg tttaccatca ttgtataaat
    56041 atgctttgaa ccagattgtc tctccggcca cataatattc tttatcgtaa tggatataga
    56101 tcttctccgt tggaaattgt gtgttcagca ctgttaatgc tgaatcaatt ttctgagcct
    56161 gcaggctaaa aaaaaccaac ataataatac ctgctgttgc aataactttt ttcatgtacg
    56221 ctgttttgcg gttaaagtac gcttttgtaa ccctctcagg gtcatcaatt atctgggcgg
    56281 aaaattggtt ggataaagat gagattttta taaatagcgc tgcctatgat tcttgttaat
    56341 tgatgtttat gatgcatatg ataattatca gaagcctgcc gtttcagcgg ttcattcagt
    56401 aaattgcata tccttatgcc attcaatctc catattccct ttccccccgc cggcgaccag
    56461 cccgcagcca tccaaaatct gactgagggg attctgaacg gagaaaaata ccagacctta
    56521 ttaggtgtta ccggtagtgg caagacctat accatcgcca atgtaattca gcaactgcaa
    56581 cgccccacat tggtgctgac acataataaa accctggtgg cacaattgta tggtgagttc
    56641 cgtcaattct ttccggagaa tgcagtcgag tattttgttt cttattatga ctactaccag
    56701 ccggaagcat atatgccggt aagcgatgta tatatcgaaa aagacctgag tattaatgat
    56761 gagctggata aactgcgcct gagagccact tccagtttac taagcggtag aagagatatt
    56821 attgtcgttg cctccgtaag ttgtatttat ggtatgggca atccaactga ttacgaaaac
    56881 ggcattatcc gtatcaataa agggcaaacc atttccaggc aggcttttct tcattcatta
    56941 gttaattctt tgtataacag gacaacagta gagtttaaca gggggacgtt ccgggtgaaa
    57001 ggtgataccg ttgacatcaa tcttccttat gttgactatg gatatcgtat cacttttttt
    57061 ggtgatgaaa ttgaggagat agaaagtatt gatgtagtta gcagcaagag gatcggcaag
    57121 atggaaaatg cggccatctt cccggctaac ttatacgtgg ctccaaaaga tattttgcaa
    57181 caggtgcttt atgaaataca ggatgaagtg gcggcgcagg cagaatattt taagagagaa
    57241 ggaaaatata ttgaagcaca acggctgact gaaagagtta actatgacct ggaaatgatc
    57301 agggaacttg gttattgcaa tgggattgaa aactattcaa gattttttga cagaagaagg
    57361 cccgggacaa gaccattctg tttattagat tattttccaa aggattacct gatggtgatt
    57421 gatgaaagtc accaaactat cccacaggta agcgggatgt atggaggtga cagaagcagg
    57481 aaactgatat tggttgacta tggtttccgt ttaccgtctg cactggataa ccgtcctttg
    57541 aattttcatg aatttgaatc cttgcagaac caggtcattt ttgtttccgc cactcctgat
    57601 gattacgaac tggaaaaatg cggtggtgta gtggtggaac aggtggtaag gcctaccgga
    57661 ctattggagc cacctattga agtaagacca tccgtgaacc agatagatga tctgctggat
    57721 gaaattgata aacgggtaac caagggcgac agggtactgg taacgacact caccaaaagg
    57781 atggcagaag aaatggacaa atatcttcac cgtatcaata tcaaatcaaa atatatacat
    57841 agtgaagtgg acaccctgga gagaatagaa attctccgtg agttacgttt gggaaaaatt
    57901 gatgtgctgg taggagttaa tttattgcgg gaaggactgg acctccccga agtatccctg
    57961 gttgcgatcc ttgatgcaga caaagaagga tttctgcgca atgaaaaatc actgacacaa
    58021 acagcaggcc gtgccgcaag aaatgtggat ggattggtga tcttttatgc agataagatg
    58081 acggacagta tgcaaaagac cattgatgaa accaaccgcc gccgggaaaa acagattgcc
    58141 tttaatattg aacacggcat tacgccaaga actgttatca aatcaaccaa agatgtattt
    58201 gcacaaacat ctgtattgga tataaaagga ta
//
LOCUS       DAORTI010000008        57946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_279_length_57946_cov_7.547892, whole genome shotgun sequence.
ACCESSION   DAORTI010000008 DAORTI010000000
VERSION     DAORTI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 57946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57946
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_57946_cov_7.547892"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLO70_02285"
     CDS             complement(<1..463)
                     /locus_tag="PLO70_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQZ73307.1"
                     /translation="MKKNFWIILFIIVLIADIIGMQTGNEVLQYIFKPLIVPVLIGYF
                     ITATSSIKSKLKKWIVLALIFSWGGDVLLMFQGQKDIFFLLGLSSFLLAHIFYIVFFH
                     HVRLSEVIKSNIWILLLVVIYYAVLIAWLSPYLGDMTLPVRIYGVVISFMLM"
     gene            complement(473..2476)
                     /gene="ispG"
                     /locus_tag="PLO70_02290"
     CDS             complement(473..2476)
                     /gene="ispG"
                     /locus_tag="PLO70_02290"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HQZ73308.1"
                     /translation="MQLYCESLTEYRRLKTREVKIGDLLLGNLNPIRVQTMTTTDTMD
                     TIATVEQSIRCIEAGAELVRITAPSKKEAENLLNIKNELKKRGYSTPLVADIHFTPNA
                     AEIAARIIEKVRVNPGNYVDKKKFEQIEYTNAEYAEEIDRIRERFTPLVKICKEYGTA
                     MRIGTNHGSLSDRIMSRYGDTANGMVESAMEFLRIARAEAYHNIVLSMKSSNPQVMVQ
                     AYRLLVKTMVDEFGECYPLHLGVTEAGDGEDGRIKSAIGIGTLLEDGLGDTIRVSLTE
                     DPELEIPVCKDLVRRYTSRESGAHGQELIPETNKISYSPFEYKRRETFAVDNIGGKHV
                     PVVIADLSKIDKILAKDLQSVGYTYNEATDKWNISDAAADYIFTGNQLLDFELPGTLK
                     VIVYPGAWSLVADKTKYFPIFEAGGFAEAEFKSDKLNFVMQDCYSDSTPVNDYTFLDK
                     MSNDPTVVICLSSTNTNAMQSVRRMFIELQNNSIRNPVVLITDSNWQTPDEHLIHFAT
                     ETGALFLEGMGDGICLGYSNKAQMESLKVTGRTYLQVKDIYQFTNNTAFSILQATRTR
                     ISKTEYISCPSCGRTLFDLQETTAKIRAVTNHLKGVKIAIMGCIVNGPGEMADADFGY
                     VGSGPGKITLYKGKEVVKKNVNSEVAVDELINLLKESEVWIDA"
     gene            complement(2511..3140)
                     /locus_tag="PLO70_02295"
     CDS             complement(2511..3140)
                     /locus_tag="PLO70_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73309.1"
                     /translation="MRKFIAIVILVSASFFAQSQVVEKATQKGNEYYKQRQFEKATEE
                     YNKALLADPENTTTKFNLANSLQKQGKQDEALKSFSELSEKAKEKELKSKSYYNKGVV
                     LTQQKKLEESIEAYKNSLRQNPDDKEARENLQKALLELKKKNPPKKKEEKENQKKKQQ
                     EQQKKQQQPKMSPKEAEQRLKLLEQKEKEVNQRLQKEKSKTGGGQVKDW"
     gene            complement(3137..4141)
                     /locus_tag="PLO70_02300"
     CDS             complement(3137..4141)
                     /locus_tag="PLO70_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQZ73310.1"
                     /translation="MSWQFQYKEFVWLFVAIAVLVLLFLSLLQWKRNTLKRIGDEKLV
                     RLLINNFSSERFTVKFILQVAAFTLGILAVMNPRKPGDADNISRKGIDIAIALDVSKS
                     MLATDLAPNRLERAKQLINKLIGAMPNDRVALVLFAGKAYLQMPLTIDHGAANLFVSS
                     ATPDAVPQQGTVISDALKMSAGVFNSNVKRFKAVVLISDGEDHDETAITTAKALAEQG
                     VMVNTIGIGSVEGSPIIDPATGLNKRDEAGNTVISKLNEEELKEIATETNGVYIQLKS
                     SDEAIAVLKSQLSQIERKAFGDVSQMNFKTYYLWFAAAMFFLLLIENFIPERKKRLTV
                     "
     gene            complement(4182..5009)
                     /locus_tag="PLO70_02305"
     CDS             complement(4182..5009)
                     /locus_tag="PLO70_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQZ73311.1"
                     /translation="MKQFSNFLLLIVFLQSCSPNNVKQDKSLKKYFDDNKVEGCFALM
                     DNASGKFTVHNLGRYRDSSYLPASTFKILNSLIGLQTGKISSDSMVIKWDGVERRVAD
                     WNKDLNMYEAFRVSSVYYYQEVARRIGKDTMQIWLDSLKYGAKTDTGKVVITSAIDSF
                     WLDNSLKITPDQQLGLVKRLYFDQLPFFKSYQEMVKRAMLFENNSNYRLGYKTGWGFK
                     ENGNAIGWVVGWVEENNHPYFFVLNIESPDKDFDMSTIRMKMLKNILKQLGFFEGRM"
     gene            complement(5041..5565)
                     /locus_tag="PLO70_02310"
     CDS             complement(5041..5565)
                     /locus_tag="PLO70_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73312.1"
                     /translation="MKLLITVLCSFFSHIISGQENPFPQNWIGNWKGELLWFKTGSKE
                     PQKVNMELRIHPADSAHKFTWQIIYGSSKEDNRPYTLIAKDTSKGHWVIDENNGIVLD
                     QFWVADKFCGAFTVMNATIINNYWIENDQLMIEFFSISAKPIMTSGNGTENSPSVDSY
                     KVGSYQKAILKRTE"
     gene            complement(5641..5769)
                     /locus_tag="PLO70_02315"
     CDS             complement(5641..5769)
                     /locus_tag="PLO70_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73313.1"
                     /translation="MKKLLVCLATAAFVMFVFSSCAATKRDCQGNKHYRLKNGIYL"
     gene            complement(6028..7215)
                     /gene="kbl"
                     /locus_tag="PLO70_02320"
     CDS             complement(6028..7215)
                     /gene="kbl"
                     /locus_tag="PLO70_02320"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQZ73314.1"
                     /translation="MNEKLVQRIAKEVEEIKSSGLYKTERIIESPQGAEIIVNGKTVL
                     NFCANNYLGLSSHPKVIEAAHKAIDLRGYGMSSVRFICGTQDIHKELEEKISTFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDALNHASIIDGVRLCKAQRFRYEHNDMNDL
                     ESKLKESATLRSRIIVTDGSFSMDGTIARLDEIVKLAEKYDACIMIDECHSSGFLGKT
                     GRGTHEYRGVMGKIDIITGTLGKALGGASGGFTSGRKEIIDMLRQKSRPYLFSNTLAP
                     SIVGASIAVLDMLTETTELRDKLEYNTKYFRTKMTDAGFDIKPGDHPIVPIMLYDAVL
                     AQTMATRLLEEGIYVIGFFFPVVAKGQARIRVQLSAAHEQQHLDKAIAAFTKIGKELG
                     VLK"
     gene            7370..7603
                     /locus_tag="PLO70_02325"
     CDS             7370..7603
                     /locus_tag="PLO70_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73315.1"
                     /translation="MNRNNQIFFFFLTILLAVSLVILRSSSSANSPKESNEQALPTCC
                     KKVKEKCPEPKKAVPADLMLESLSRQLITISAF"
     gene            7745..8230
                     /locus_tag="PLO70_02330"
     CDS             7745..8230
                     /locus_tag="PLO70_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ73316.1"
                     /translation="MTEREYNECVNTYADNVYRFILKNLRHEEDARDVVQTAFEKMWR
                     NRDDVDAQKSKSYLFTVAYHQMIDHIRKVKRIQLREEFSAEARVENRPANNLKKILDE
                     ALNRLGETQRSLVLLKDYEGYSYEEIGQIMNLNPSQVKVYLHRARVQLKEYLVKIENV
                     I"
     gene            8304..9431
                     /locus_tag="PLO70_02335"
     CDS             8304..9431
                     /locus_tag="PLO70_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73317.1"
                     /translation="MNINRHNYEECFILYMDNELSSDERRMVEEFIQHHPDLKEELEI
                     LQQFKLSPDTDIIFEGKEELLKGQENPSLNLSNYEESLLLYTDNELNAAQKTMVEQFV
                     KENPSIKKEMELLQRTKFQPEHIVFAHKESLYRREEKVKALPVRWWRAAAAILILGLS
                     LTTALLFNNKKAPEPAEMAKTTGNEEKNVPAGHQNKENAIAVVEEVKAPGNNPEEKNN
                     TTVYAPDNKQSTNQVAVKNNRAENPIPRNSIVPVKNNEPLLADNLTGTNNLPKPESNP
                     YVNSDLPRTEIAGNIPKEKNTLHPSLTNPVVTNRTTSPSDFVQASNTISDEDVNDAGG
                     KKNKLRGLFRKVTRTFEKRTNIDPTDDDNRLLVGGLAIRLK"
     gene            9461..10303
                     /locus_tag="PLO70_02340"
     CDS             9461..10303
                     /locus_tag="PLO70_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ73318.1"
                     /translation="MKRIFTLCVALCTIASSFAQTDTTATPQPAPQDDTIRIGGMVII
                     RKAGSKDKEIIRDKEYKMRNRRTDKPSNLSTNWWIFDLGFSNYNDQSNYTAAAVSGFV
                     APGIGEDQLKLRAGKSRNVNVWFFMQKLNIAKHVLNLKYGMGIELNNYHFENDKIVFA
                     KNPTYISQGTTEFKKVKLAADYLTVPMMLNINFTPKRKNGFGLSGGISAGYLYSARYK
                     TKDGKDVEKVKSDFDLERFKLSYIGELSLGPVRLYGSYAMKNMWEKGLDMTPYTLGFR
                     FSNW"
     gene            10568..11134
                     /locus_tag="PLO70_02345"
     CDS             10568..11134
                     /locus_tag="PLO70_02345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQZ73319.1"
                     /translation="MKNLFILTSVFLGSSLLATDVHTGKRTAINKRFSGTLYKGPVGT
                     ISIIIDKSDYELNVYDDKGWYATYPVVFGNSSLADKKMEGDKNTPEGTYRITAKRVHE
                     KWCRFLSIDYPTAADRAKFNQRKQRGEIPANARIGGGIGIHGTWPHEGFVIDRYKNWT
                     LGCISMKNEDVTEIYQFAGAGTTVTIKK"
     gene            complement(11138..12118)
                     /locus_tag="PLO70_02350"
     CDS             complement(11138..12118)
                     /locus_tag="PLO70_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ73320.1"
                     /translation="MEEKKTRQKWPSILRWTLWVILVQIVLINISAAFYAYRLTHFYN
                     DPSLRTSQPSGNIFTKTWKLFTGPKFPKSQINDVPVFIYDTVSLQTENGLTIDAWYSK
                     TDSVAKGTILLFHGITSNKGQLLTEANEFRYLGYNVMLVDFRAHGNSDGSTTTIGIRE
                     TEEVKLAYDFVKGKGEKKIFLWGISMGAVVIAKALSEYSIYPAAVILELPFSSLQSLL
                     KARARVLGFPQQPFAFLVTGWMGIERGFNGYRYTTDRYAKKISCPVLVQYGAKDSYVL
                     KSEIDKVFTAIFSSDKKLVGYENADHESLLQNDPVKWRMEVGNFLGKNSQ"
     gene            complement(12339..14276)
                     /gene="acs"
                     /locus_tag="PLO70_02355"
     CDS             complement(12339..14276)
                     /gene="acs"
                     /locus_tag="PLO70_02355"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQZ73321.1"
                     /translation="MSYPYQIKSLEQYHETYKKSVEDPEGFWATVAENFQWRKKWDKV
                     LEWNFKEPKVEWFKGAKLNITENCIDRHLETMGDKPAIIWEPNNPEERVRIVTYNRLH
                     KRVCQFAQVLINNGVKKGDRVCIYMGMVPELAYAVLGCARVGAIHSVIFGGFSAQSIA
                     DRLYDAQAEFIVTCDGAYRGGKDIPLKSVIDDALIGNRTVKKVIVYTRTRTPVSMLKG
                     RDVWWEDEMEHAEAQVEKNGKVSFPAEEMDAEDPLFILHTSGSTGKPKGVVHSTAGYM
                     VWTTYTFVNVFQYNPGDIHFCTADIGWITGHSYILYGPLSAGATSVMFEGIPTWPDAG
                     RFWDIVDRHKVNILYTAPTAIRSLMGFGLEPLKGKDLTSLKILGTVGEPINEEAWHWY
                     DDNIGKRKCPIVDTWWQTETSGCLISNLANVTPSKPSWATLPMPGVQPVLVDENGKEV
                     TEKDGQGLLKGNLCIKAPWPGIIRTTYGDHERCRTNYFAAYENLYFTGDGALKDEEGN
                     YRITGRVDDVLNVSGHRLGTAEVENAINMHAGVVESAVVGYPHDIKGQGVYAFVIYEH
                     MHVDENLTRQDILQTVTRVIGPIAKPDKIQFVTGLPKTRSGKIMRRILRKIAEGETEN
                     LGDITTLLDPGVVEEIKKGKL"
     gene            14412..15965
                     /locus_tag="PLO70_02360"
     CDS             14412..15965
                     /locus_tag="PLO70_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ73322.1"
                     /translation="MKPTTIRSVIAASSVGTMIEWYDFYIFGMLAKTISTQFFPEGNA
                     TAALLSTLAIFAAGFIVRPFGALVFGRLGDLIGRKYTFLLTLVLMGGSTFLIGLVPGY
                     KSIGIAAPLLVLLLRLIQGLALGGEYGGAATYVAEHAPANKRGFYTSWIQTTATLGLF
                     VALGMIMLVKLNMSESSFNAEWGGWRYPFWISILLVAVSVYIRLKMSESPLFSKLKEE
                     GKTSVNPLKESFSHKANFKMVLMALFGAVMGQGVVWYTGQFYAQNFLETKAMLDFEQS
                     RTILLWAIAFATPFFVFWGWLSDKIGRKWIMMAGMALAIFTYRPIFNTFLADADLPAA
                     IIKKQLPPETVKIEPVIIKADTVKREAGVIIINHIHSKHTLPDGSRFSASYTDSIFNN
                     SNGVKNEVKTSPITYVNKHLPSSLYWKFIGLVFLLILYVTMVYGPIAAFLVEMFPTKI
                     RYTSMSLPYHIGNGVFGGLVPFIGLLLTTSFPSDPLVGLWYPIGVAALCLLIGIVYLR
                     NKIDENVED"
     gene            15976..16224
                     /locus_tag="PLO70_02365"
     CDS             15976..16224
                     /locus_tag="PLO70_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73323.1"
                     /translation="MNNIKRALGIIWLLLAPAVIYFLVDRAIVHIDPAGTKDINNPVI
                     WIIIIGIFTPIAIGLMIFGWYAFKGEYDHLPESSEELS"
     gene            complement(16191..17399)
                     /gene="nhaA"
                     /locus_tag="PLO70_02370"
     CDS             complement(16191..17399)
                     /gene="nhaA"
                     /locus_tag="PLO70_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQZ73324.1"
                     /translation="MEVKTKEKDLNDFGLSRLFSEFFNSEKAAGFILIGCTVLSLVIA
                     NSPWGEGYDQFWHTRIGGLSISHWINDALMAVFFLLVGLEIEREIYVGELSQLRNAIF
                     PIVAAAGGMLVPAAIHAIFNFGTATATGFGIPMATDIAFALGILSLAGKSVPAALKIF
                     LTALAIIDDLGAIMVIAIFYTEDLSVLNLFISLGIFVVLFMMGKTGVKNLLFYIIPGI
                     AMWYFMYQSGVHATISGVLLAFAIPFGKGGAHSPSFKLQHILHYPVAFFIVPLFALAN
                     TGIKLQPGFYNTLVSVNSLGIMSGLVIGKPLGILTFAWLAVKTKFSSLPSSVTWKHIA
                     AAGLLAGIGFTMSIFITLLAFADNETVVNSKIAILGASLVAAIAGLLMLKSINSKTVN
                     QESSSEDSGR"
     gene            17568..17861
                     /locus_tag="PLO70_02375"
     CDS             17568..17861
                     /locus_tag="PLO70_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73325.1"
                     /translation="MKLVAVILFFVFAGTVFAPVAQSLCKDSQISLFIVDEEKSNTQI
                     AENEDTKKEQSKTIFYQLILASISEKGDAAFLNSADLPNSPYRDYSTPPPDFV"
     gene            17925..19568
                     /locus_tag="PLO70_02380"
     CDS             17925..19568
                     /locus_tag="PLO70_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ73326.1"
                     /translation="MEKSLSPVARIWNLVRQEKTEITAIYFYAILAGLIQLSLPVGVQ
                     AIISFVLGGVMSTSLILLITLVVTGVLATGLMYINQMKLIEKIQQKIFVRYTAEIADR
                     IPRLDLKKIDSFYLPELVNRFFEIPVLQKGLSKLLLDIPTATIQILFGLLLLSFYHPV
                     FILFGILLILVLAIILYYTGSKGLKTSLAESGYKYAVAAWLEELARVVKSFKLSKGTS
                     LHLKRTDERTITYLNTRTQHFGVLLFQYRTLVALKTIITAAMLLVGSFLLVNQQLNIG
                     QFIAAEIVILIVISAVEKIIGTLDSVYDVLTSVEKIGKLTDKPLEQHGNHLLPQKKEG
                     VSIEFAAVQFGFSNNSHIIDDLNFRIEAGETACISGKEGSGKSILLHLLGGVYTDFNG
                     SILVDEIPINNYNIDSLRSQTGIYLSHQDIFHGSLLENITMGNNNIGMDEISRWVEKL
                     GLKSFISSLKYGFDTQLDPLGDHLPGNVVRKILLLRALINKPRLILLEEPWLGFTDEV
                     KNKIQEALINDTAGSTVMVISNDESFASKCSKRIQLNRQ"
     gene            19579..20889
                     /locus_tag="PLO70_02385"
     CDS             19579..20889
                     /locus_tag="PLO70_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQZ73327.1"
                     /translation="MESKNYDIKAYSHIYRYNSRSRVKYWLYGLIIFNTALLFLPWTQ
                     NIRAKGNVTTLRQEQRPQQINTIIPGKVVKWYVKEGDFVNQGDTILQLAEIKDEYLDP
                     NLISRTGDQLSAKEMKVEYYKSKAGTASTQINALQNEQQLKLEQTRNKITQQERKIES
                     DNLDFMAAVNELSVAGRQLAAGKAMLDSGVISLIEYEKRKVTYQNAQAKKTGAENKLA
                     NAKQELAILNIELNAISQEFTEKISKASGDRFQSMSEVATGQGDIAKLQNQFTNYNIR
                     SGMYYVTAPQSGQVSKAKKAGLGEILKDGEMIVEIVPDKIQFAVEIFVRPVDLPLINP
                     GQKVRFWFDGFPAIVFSGWPNASYGTFGGKVFAVENSVSSNGKFRILVAEDPAERPWP
                     KELRIGAGAQGMALLKDVPVWYELWRNINGFPPDYYKVDKTEKK"
     gene            20916..22388
                     /locus_tag="PLO70_02390"
     CDS             20916..22388
                     /locus_tag="PLO70_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ73328.1"
                     /translation="MKTQKKYILLYWIKKRREKRNTIIKTTIIGITLMLLALVAAAQD
                     TIKTLSPQQLAAIIRLYHPVARQADINIEKAKAGIITAKGGFDPLLTNATAQKTFDGN
                     NYYYYNRPEITIPTWYGISINGGLEYLSGNRTDPRDTKGESSFIGITIPLAKNLLMDK
                     RRAALQTAKIMKEASAVEKRSIINTLMQEALTMYWNWVKHYLIYKLASDAIAINEKRI
                     ELIRISFRQGDRPALDTIEALTQLQNFQLWQSQARLDFQNMGLELSNYLWAADNTPFF
                     LQPDVIPDEESQMLKADSFVTPDLAQILETIRDQHPDLLQYQFKLSALDIEKKLKFQE
                     ILPTVNFRYNQLGRGYDIAKTITNPLFENSFQYGISIGIPLRLSAGRGEYKTAKLKIT
                     ETKLQQYQKIQQLENKIKSYYNELAALKTQVSLYEKAVQNYNTLLKGEETRLLNGESS
                     LFLVNNRESKTLEALQKLTELKVKYFQANSLLLGTAGMLN"
     gene            complement(22444..24300)
                     /locus_tag="PLO70_02395"
     CDS             complement(22444..24300)
                     /locus_tag="PLO70_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQZ73329.1"
                     /translation="MNQLLILFIAIPMLAFFASLVWQNKSERPIGYIVRVTKIFYIVV
                     SISFFIWWMLEGYKPVSYKLATLYQTDDFVFAIQLYYDEITAVFSVVGALLFFLVATF
                     SKYYMHRDQGYKRFFNTILLFATAYNFIILSGNFETLFIGWEIKGICSFLLIAFYRNR
                     YLPVKNAFKAVSYYRISDVAIMLAMWMMHHLTHQNITFAQLGEAKNTAIALDQPGMAI
                     FIVCMLILPAAIKSAQFPFTTWLPRAMEGPTSSSAIFYGSLSVHIGIFLLLRTHPFWE
                     DMLWAKVLIIIIGGLTAVVATMIARVQPTVKTQIAYSSAAQIGLMFIEIALGFHWLVL
                     IHFAGNAFLRTYQLLVSPSVLNYLVHHQYFHYTLPMQKPVSRIKASLYILGIKEWNLD
                     TIMFNFLWSPFKWIGKKFQFLHSTVFVAVLLFTGLGVLALGYFSPTVVQTFGGILPLT
                     LMSIALSVILFSFSYRKSALKAWIYLLTGHLFIIAAILFNVPHVNFIEIIFYASGVLA
                     AFMLGYYCLHKIKTIDNDISLNKFHGYVYEEETTGFLFLLSAMGILGFPLTAAFVGID
                     VLFTYVHSNQVALIILMALCFVFIELAAFRILLRVFFGPHKKLNHPVAFRSS"
     gene            complement(24376..26946)
                     /locus_tag="PLO70_02400"
     CDS             complement(24376..26946)
                     /locus_tag="PLO70_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HQZ73330.1"
                     /translation="MDNHSFNEAHVLHELKHYLPSQQALRDFIHHNSLHAFQHMKFYD
                     AIFTASKIFGFQVHLQLPEYREMYKTGRIREDILQMVIAGKKGKNAVADWKKNLISKE
                     YDTINQPRIGQLRKHWKDVYGLDLDNKVHPLLFRILCTFLDQGIAIEGFPVVNKSFID
                     SIKELEQNSFVSFFRTKAVRQQFLSGQYTITDLLQVIVGDSAYFEQYLFDQQFAHHGW
                     SGFVSAVEDNPHTLLDRKNITFKELVEFELLMELDALNHALGNKWQPLASHVSAPPVN
                     FFAEVHKTEFAQVIELWQDAFEWSYYDSVLKGISLADSKRILNDKGLNKFSLTDKTVG
                     PSLQAIFCIDEREDSIRRHIEAVDKSAETFGAPGFFGVEFYFQQQGSKFYDKLCPAPV
                     TPKYLIKESDSKSLQKEELIYTKYTHGILSGFFLSIGFGLWAFVKKMQMMFRPKMSPA
                     ISNAYGHMDKQSSLTIENKHLSDIENGLQVGYTLEEMAARGEGFLRGIGLLKDFAPIV
                     YIVAHGSSSANNPHHGAHDCGACSGRPGATNARVQAFILNHTKIREILSGKGINIPAS
                     TQFVGAMHDTAADVMAYYDEKILSEENTKRHIINIQNFETALNLNAKERSRRFASINT
                     KQELEQVRKAIHSRSVSLFEPRPELGHGTNTLAIIGRRQVTKGLFLDRRAFLNSYDHT
                     TDADGTILAAVMRPIGLVCGGINLEYYFSRVDNIKLGAGTKLPHNVMGLFGVANSSDG
                     DLRPGLPWQMIEVHDPVRLLVIVEHRPDIVLKAIQSSPEVFEWYNNEWVHIVALHPEE
                     KQFYYFRNGAFTKYEPITDAGDIKTIHNMEEFIEGAKEMETNHIVHATEENLPVYLLD
                     "
     gene            complement(26988..27677)
                     /locus_tag="PLO70_02405"
     CDS             complement(26988..27677)
                     /locus_tag="PLO70_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQZ73331.1"
                     /translation="MRAHSKETQASLTPRMAMEILQEGNGRFIKNLKAQRDLLEQVND
                     TREGQWPFATILSCIDSRTSAELVFDQGLGDIFSIRIAGNIVNTDILGSMEFACKVAG
                     SKLIVVLGHTKCGAVKGACDHVEMGNLTELLSKLQPAVYSESETTAADKRNSKNGKFV
                     ENVATLNVKRSVKNIIERSFILEEMLKKGEIGIVGAMYNIDTGKVEFYKDVSYIKDEK
                     NPKFSVAELRH"
     gene            complement(27715..29265)
                     /locus_tag="PLO70_02410"
     CDS             complement(27715..29265)
                     /locus_tag="PLO70_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQZ73332.1"
                     /translation="MKNNSMFKEIKSDLPASIVVFFVAVPLCLGIALASGAPLFSGII
                     AGIVGGIIVGIASGSSLGVSGPAAGLAVIVLTSIATLGSWPAFLLAVVLAGFIQLALG
                     FAKAGFIAYFFPSSVIKGMLTGIGLLIILKQIPHALGWDKDAEGDDAFLQADGQNTFS
                     EIAQALDFITPGAVLIAAISLGLLILWDSVLTKKHKIFQLIQGPIVVVILGIVMNYLF
                     KSGTLDFSLADDQVVRLPVANNLTEFFNQFTLPDFSAITNVEVWKIAIVLAIVASLET
                     LLSVEATDKMDPNKRITPTNRELKAQGLGNIFSGLIGGLPVTQVIVRSSANITFGGKT
                     KLSAILHGIFLLISAITIASVLNMIPLASLAAVLLMVGYKLAKPELFKKMYKLGWEQF
                     IPFVATVIGILLTDLLKGITIGMLFGIFYTLRHSFRNSHYMKETVTSEEGHEVHHLVL
                     AEEVSFFNKASVIKELEEIPENSKVIIDCSNSKSIAYDVVELIRDFKSNAKTKNIAVE
                     TINFIEPA"
     gene            complement(29390..29746)
                     /locus_tag="PLO70_02415"
     CDS             complement(29390..29746)
                     /locus_tag="PLO70_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73333.1"
                     /translation="MGKNSKTYNTFQVASAIFMILALLWLTVSTPFVFNSQKERSSQQ
                     LSANAGTEEETANPFGNTTEEKTPNSSSFSEEYLHDHHGADHFLLIVSQYHKCENSGT
                     YTAFHGELLVPPPNQL"
     gene            29977..30612
                     /locus_tag="PLO70_02420"
     CDS             29977..30612
                     /locus_tag="PLO70_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQZ73334.1"
                     /translation="MKPYEVLLNDNKHWAAKKLAEDPDFFERLLHVQSPEFLWIGCSD
                     SRVPPDQITQTEPGEIFIHRNVANLVVNTDVNLMSVVDYAVNHLKIKHVVVCGHYGCG
                     GIKAAMSNEDFNQVLNMWLRNIKDIYRIHRKELENIPDEEKRVNRMVELNVKEQVFNL
                     AKTALIQRAWSSEQRPDLHGWVYDLHDGLIKPIVEMKAGTDIDPLYEFEDL"
     gene            complement(30620..31903)
                     /gene="nhaD"
                     /locus_tag="PLO70_02425"
     CDS             complement(30620..31903)
                     /gene="nhaD"
                     /locus_tag="PLO70_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQZ73335.1"
                     /translation="MITVISIIFIAGYAAIALEHPLKVNKAATALITGVLCWTVFALF
                     SDNEHAPAHQLTEYLGEVAGILFFLMGAMTIVELIDAHDGFEVITSRIRTTNKRKLIW
                     VIGLITFFLSAVLDNLTTTIVLVSLLRIIIPDKKDRLLFAGMVIISANAGGAWSPIGD
                     VTTTMLWIGGQITAGNIIIKTLLPSLVCFLVPAIIISRQLKGHIGQSVEPIKNKDFTT
                     NSRERKTVFWLGLGSLLFVPVFKTITHLPPYMGVLLGLGVMWVITEMIHSGKDEAEKG
                     LYSVNHALRKIDTPSILFFLGILLSIAALQVTGVLADLAVSMNNSIGNIKIITVLIGL
                     LSAIVDNVPLVAAAQGMYSLEQFPTDHYFWEFLAYCAGTGGSILVIGSAAGVAAMGLE
                     KIEFFWYMKKIGWLALLGYLAGAGVYLLQLYLFHS"
     gene            31980..32897
                     /gene="gldA"
                     /locus_tag="PLO70_02430"
     CDS             31980..32897
                     /gene="gldA"
                     /locus_tag="PLO70_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HQZ73336.1"
                     /translation="MSIEVKNLLKQYGEQKAVNNISFKVDKGEIVGFLGPNGAGKSTT
                     MKIITGYLAQTSGEAYVCGINVAEEPLETKKKIGYLPELNALYYDMYVKEYLAFVAEL
                     HKIDDKRKAVEKVIETVGLTIEAKKKIGQLSKGYKQRVGLAAALIHGPEVLILDEPTS
                     GLDPNQIVEIREVIKQQGKDKTILFSSHILQEVEAICDRVIIINKGILVADDKMSKLS
                     KTSSNIVKVTFRESIDIQLLEKLPAVTGLTKTDTNNWQLATHDSDQLRKQILELALRH
                     NLNIVSLQSENQSLEDVFRSLTQSHTIQS"
     gene            32914..34203
                     /gene="eno"
                     /locus_tag="PLO70_02435"
     CDS             32914..34203
                     /gene="eno"
                     /locus_tag="PLO70_02435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQZ73337.1"
                     /translation="MSYISEIFARQILDSRGNPTVEVDVLTDEGALGRAAVPSGASTG
                     IHEAVELRDNDKKKYGGKGVLKAVANVNKTIAPALLGYDVADQTGIDEMMIQLDGTPN
                     KGKLGANAILAVSMAAAKAAAEEASLPLYRYIGGTNAKVLPVPMMNILNGGAHADNKI
                     DFQEFMVMPVGAPSFNEGLRWGVEIFHALKTVLKKKGYSTNVGDEGGFAPDIQSNEEA
                     IDTVMLAIKTAGFKAGTEVAIAMDPAVSEMYDSAKKVYHFHKSDGKKLTSEKMVDYWA
                     NWAKNYPIVSIEDGMAEDDWSGWKLLTDKLGKKIQLVGDDLFVTNVIRLQKGIETGVG
                     NALLVKVNQIGTITETINAVTLAQHNGYNTIMSHRSGETEDTTIADLAVALNCGQIKT
                     GSASRTDRIAKYNQLVRIEELLGSSAVYPKGKIKFGK"
     gene            34280..34594
                     /locus_tag="PLO70_02440"
     CDS             34280..34594
                     /locus_tag="PLO70_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQZ73338.1"
                     /translation="MKLLTHIPSWIKSKYFIAFAAFCVVVLFLDKNDFFTQIDRQKEL
                     RELQQSKQYYSTQISAERKELEALKNNPATLEKYAREKYLMKRDNEELFLVSEKPDNS
                     KN"
     gene            34707..34940
                     /locus_tag="PLO70_02445"
     CDS             34707..34940
                     /locus_tag="PLO70_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73339.1"
                     /translation="MTNFTPEDLLLYLYNETTSKQTAAIEKALEKDWTLREKLSVLKV
                     SMQRLDKITESPRTEVVLNVLNYAREQVVQEIQ"
     gene            35054..35698
                     /gene="nth"
                     /locus_tag="PLO70_02450"
     CDS             35054..35698
                     /gene="nth"
                     /locus_tag="PLO70_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQZ73340.1"
                     /translation="MTRKEKYQFVIDYFQQHAPEAETELLYDTPYQLLVAVILSAQCT
                     DKRVNMTTPAIFKKYPTVDALSKATFDELFPLIKSISYPNNKTKHLIGMAVKVLGSFH
                     GKIPMTVDELVQLPGVGRKTANVITSVIDQQPNMAVDTHVFRVSKRIGLVPQTASTPF
                     AVEKILVKNIPEKLIHKAHHWLILHGRYVCLARNPQCEKCGLQQVCSYYLREKR"
     gene            35857..37734
                     /locus_tag="PLO70_02455"
     CDS             35857..37734
                     /locus_tag="PLO70_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73341.1"
                     /translation="MKAQIPPRQWAQHYGGANVDIPYTIKFTADGGTVVAGYTDSKSG
                     DVSAQPNREYWDLWVLKLDKCGTIQWEKSFGGRGYESARDIVQTADGGYMVLGETNST
                     DGGVIAGYGGTKDIWLLKLSASGNLEWQKRYGGSGLDIGNHIELTTDGSFLIAATSSS
                     NDGDIRGNHSTGTFTDGVLMKINAAGNLLWSKCFGGSKNEELFDIEVINGTIFAVGFT
                     NSIDGDIPPDQKNYDMWLLAIDAGGNKVLSKIYGGSQNDVAYCMTKGANGSLTLAGYT
                     TSSDGDVSGAKGSQDYWVINLDQKGKLNWQKVLGGTDAEYAKTILTDSDSSYIIGGVS
                     YSNDGDINGALGEGDFWTVKLSPTGNEVWKKNWGGNRNDHLRFMIRNPARNEYYLAGD
                     SESGDGDFNNTIGDADFAIIKLKIPELLTQDSAVCNINGFIPFEDTLYDICGFDSAIV
                     KYNPVVLNGPFESIRKSDTVFAGQSVTLHANGNGIISWNTHSSLSCTDCSNPVATPQV
                     TTVYTATNRSPDGCQVSDQFTVVVLNDALVNIPTGFTPNADGRNDHFGPLGKVPDGYR
                     LQIYNRNGEVVFKSSAINQRWDGRYKGMLQPTGVFIYLVDYKDLQNKPHQQKGTFVLI
                     R"
     gene            37761..39398
                     /locus_tag="PLO70_02460"
     CDS             37761..39398
                     /locus_tag="PLO70_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQZ73342.1"
                     /translation="MDRRDFFKRTRRKYSTTSNANQYGKEMFAGLNPYTGNWTVNEVT
                     HLLKRTMFGARKTDIDYFLSLSPGAAVDELLNTVMVPDPPVRDYGLLEDDGVFYDDLG
                     VAIGQTWVNDLNMASAPEIRGQINSRRASSLKKWWAGLIINQHRSIQEKMVLFWHHHF
                     SIQESEVDNAQILYRHHHLLRSNALGNFKSLVKEVTIDPAMLIHLNGYLNSRQAPDEN
                     YARELQELFSIGKGDDSHYTEDDVIAAARVLTGWRINDNPLGSYLDAGAHDTGPKTFS
                     SFYNNTTISGSTDPDQELDALIDMIFTTTEAARFICRKLYKWFVYYDIDETTETSVII
                     PMAEILQSNNYDVKPALAVLFKSEHFYDVLNQACYIKNPYDMIVGTLREFNVSFPSYT
                     DYITGYPLFYSIYQKAAEMQQQLFQPPDVSGWPSYYQEPMHYELWVNSNSLPKRADFT
                     DALVNDSVIDVRAFANYSSNPADPNQLINDVTALLLRYPLSNDSRVYIKTHFLTNNTT
                     DDTIWTNAWNSNNTTVINTSLRELFKFLMNLPEFHLC"
     gene            39449..41050
                     /locus_tag="PLO70_02465"
     CDS             39449..41050
                     /locus_tag="PLO70_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQZ73343.1"
                     /translation="MNRRKFLRNTVPVAVTMPALLNGFSFTAQGAESGLARLLEQTLT
                     DTDHVFVLVQLAGGNDGLNMVIPLDVYGNYYNARTNVAIPQSQVLRLDGTDKSGLHPA
                     MTAMQNMFNDGKMSIVQSVGYANPNFSHFRATDIWNSGDPRENRRERVKTGWMGRYLE
                     SEYPGYPDAYPDATMPDPLAIQISNFPTLTVQGSVYSMGLSITNPEKVYTFDNPFSDY
                     PLNAAPANKELKFLRVISEKTKIYSDIIRTAYQRVATLAAYPSENVLADQLKIVARLI
                     KGGLKTRVYTVTMGGFDTHKRQVNASDTTTGFHARLMKDLSAGISAFQQDLELMGLEE
                     RVMGMTFSEFGRRIKSNASLGTDHGAAAPLIIFGKHAKKGILGNSPDIPADIQVVNNI
                     PFQYDFRSVYASILEQWFCVKQPALNSIMLQNYQSLPLITGPCGVADDPDDTNNNSNN
                     LLLKMWPNPYNVSATIQFSTIGGHTMIQQIDALGRVIKVLTNQEYAAGTYNVDIYNDG
                     LASGVYFLRLQNKSLQKVISVMKLP"
     gene            41263..42189
                     /locus_tag="PLO70_02470"
     CDS             41263..42189
                     /locus_tag="PLO70_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQZ73344.1"
                     /translation="MKKLLILGLAFCFVQDQPVKAQYYYYNDRYYDNDVVMEIGATFG
                     IMNAFTDLGGKKGIGKSFIKDLNWKNSKASYGLYAAAMYKNVLGLRVEATFGQVAAYD
                     SILKDVKATTFGRYERNLSFKSRIADIQLGIEVHPLFFKNYYEGDEDPPFISPYVVAG
                     IGYFSFDPQAKLNGQWYALQPLSTEGQGFAEYPDRKPYKLSQLNIAAGLGVKYEVNSF
                     INARLEINHRFLNTDYLDDVSEDSYIDPALFNNYLSSNRAAIAQQLFDRRAEVDPGHT
                     TNPSYQRGNPKDKDAFFTIQLKIGINIGRQRR"
     gene            42411..44795
                     /locus_tag="PLO70_02475"
     CDS             42411..44795
                     /locus_tag="PLO70_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73345.1"
                     /translation="MDDTNTFIKDPVFNSLLSFRPLVNALKKNIAEGNPGMKKLYGHV
                     VQEFESHPELMGTITDLDVLQPYSELIEELLSAVFPPTTANYMYGISFPFKNLAVYAS
                     PLFKTLLVKKDSAEIIIQDDSTSTLNRERLHFAFGLILKKYLGYNSADSSKVVYPFTD
                     EKTGLVRYMEMRLDGRFIDVRPVDEMPKMPESIFDYTSNRLLTIEELMQQVSLDKFVF
                     EGLTVIRINDVTEQAVIAEMKNKLLDSNFVSDNTAYTDLEKQIQNLIGLKDLTIGLTP
                     FFKINGHYVYSNLHNNNSLLFRHLQSTGEKDEISDYCKLLFRYNNQPVLYESLTEDIL
                     GEVQCLPYYHKAGSRCMIICPLKQQNELLGMLEISSKQPGYLKPEHISKLEAAIPMFT
                     LGLEKSLEQLNNQVDKVIKKKFTAVQPAVEWKFTEASLNYIVNMYEKQDSGIERIVFD
                     DVFPLYGAIDVRNSSTARSHSVQLDMLEQLELARKVVRKAQAAMSFPLLQEIEFKIDR
                     HIASASDVLQSEEEMSVYDFLQGQVISVFNHLRDTEPAVKNEIEEYFSSLDPQLGMLY
                     KHRKEYEDSISLINHTLARFIDKEQAAAQKVYPHYFERYITDGLEFNIFMGQSISPRK
                     KFDEIYLRNMKMWQLTVLTKAAKVTHKLEQELSQVLRTTQLILAHSQPLSISFRTEER
                     KFDVDGAYNTRYEIVKKRIDKVRIKETNERLTQPGKIAIVYSQAKDAAEYTEYIEFLQ
                     SKNLIKPGIEKHDLEELQGVSGLKALRVDVDFDTEPRMEKKIELSATTSDQLLR"
     gene            complement(44860..47436)
                     /locus_tag="PLO70_02480"
     CDS             complement(44860..47436)
                     /locus_tag="PLO70_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73346.1"
                     /translation="MRPTKLLILACFVVPLNYCAAQKSTDSASAKPSSFSTSGGRIFW
                     MGANYRKEWNTPVKAPVLNLATESGGLTPLKRGGGKQTKSLRLEDPNGKQYTLRTIQK
                     FITDKTLPGDLQSEAAADLVSDGVSASYPYASLSVIRLAEAAGIPYGKVKLVYIPDDP
                     RLGENRMEFANMLATLEEREPAGIKKVFDTDEVAEKLEKDNDNTVDQLALLKIRLLDM
                     FVMDLDRHEGQWLWGVNDIDDGKGKQFFPIAKDRDQAFYINQGLLPGLIKSRSMVPQL
                     EGFKSEANNIGRFNFAARNLDRFFLNQLTEEQWKQAAEKFVSQMTDEVIDRAMAQQPA
                     EIRSISAPKIAQTLKDRRNFLVAEAVEYHRFIAEIVNVTGSDKKELFDISRNDDGSVL
                     VQVYKINREGQQSVKMYERKFDPAVTKEIRLYAMDGDDKYLLRGSNDKIKLRLIGGGG
                     SDVFENTSKSQRGALLYDKLNGDNKVMGPFKNKMSNDSTVNSFERIYYKYPFQSFFGT
                     IGYNPDDGLSLGPTMKIIRHGFRKSPYKSLHQFSGKYAFSTKAVNLRYNNEFIDVMGK
                     NTDLVMDFDYKGPFTTTNFFGYGMNSVYDKTQPDRFRFYRIRYDLGDFNIQFRHRFSD
                     KVAFLFGPTFQFYHLEADDKFNQQRNIVLNTVASGLDPSKVFNRQSYLGGLFSLIIDT
                     RNNKVLPESGVNWISTVRYLDGLNDNSYDNVTQLNSSFAFYFNLLAPGRLVFANRVGG
                     GINLGDGFEFFQAQYLGNEDNLRGYRQYRFAGKSKFFNQSELRLKIADFRTYLFPGSI
                     GIAAYYDVGRVWVENDLVDKWASGYGLGFWVSPLRRLLFSFVYAMSKEDKLPLVTLGW
                     RF"
     gene            47770..51483
                     /locus_tag="PLO70_02485"
     CDS             47770..51483
                     /locus_tag="PLO70_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ73347.1"
                     /translation="MKYIGLFGLVLILNLSINAQSDTITQRIVLIGDAGQLTNGRHPV
                     VDAVKKNIPLDSKTTVLFLGDNLYKSGLPDDQYALQYLKAKAVLDSQISIADGTPAQV
                     YMIPGNHDWDNGRAGGFDAIIRQQLYVDFLQKPNVKYYPKDGCPGPEEIDLGEDITLI
                     LFDSQWWLHPHDKPEIESDCSCKTKEELVTQIGDIAARNAKKLIIVACHHPFESNGMH
                     GGFYTLKQHIFPLTDIRKNLYIPLPIIGSIYPIARSVFGTPQDTKHPNYANMIEQVSD
                     AVKSSSPNVVFVSGHDHNLQLIRENNYNYIVSGGGCKQNRTSNNTNSLFNSTSEGFCV
                     MEVSANKNVSVAFYTVGDSVTQAYNKPLLNFSSTADTAYTTSNLNNPDLNKDSITKAS
                     SKNYPLAKGFKKFIIGQNYRPEWSAPVNMKVFKIAEEKNGLTVISLGGGNQTQSLRLK
                     DKNGKEWVLRTVDKMATKAIPRNLRGTFAEDIALELNSASHPYAPLAIPGLADALDIA
                     APHPELFFVPDDPSLGFYRKIFANKICMLEERHATNDTLEMKMVKRFFSQLPKEIHLS
                     KDTSETKTTTKFFDKMLEENDHRPIQQEVLKARLLDIVVGDFDRHLDQWRWGSIDTGK
                     GKIYYPIPRDRDQAFFNSDGFLLKALSRRALPFLKGFRYDIPKVNWLGYGAKDFDRLF
                     LTDLDDTEWEKTIAEVQQKLTDSVIRKAVWNMPPEISRISGETIARKMMSRRDILATE
                     AMNYYRFISRKVNILGSNKKEYFKISKYGDGLQVKVFARTKKNDTSFVMYSRVFNPST
                     TKELRLYGLNDDDIFDIEDNVTSRIKIRIIGGKGYDTFDIKGNVETLLYDLKDGGNHI
                     KNNSRAKNRFSTEAPVNDKDIFGFNYNTTRFPQLHFGQNTDDGFWIGGAFSKRTFGFR
                     NLPYASDQRLAALYSVSRKAWQFNYVGEFNHITRKTDLVIKANFSTPVLRNFFGFGNN
                     TEIDLSKPKDFYQSRYKLFETEALLRKRFFEKLHIMAGPAFYSYRNSIEDNRNNFFGK
                     PVQNGLDSAGIYSKKDYLGGKLVLLLDNRNDELFPTRGIHWQNEFVSIAGLKKGNYNY
                     TAFTSDMSVYASFTDPAKFVAVLKFGGGHIFSKNYEYFQALTLGANNNLPGFRKNRYA
                     GTSTLYSGLELRVKLFGMQSNMLPGQLGLTGFYDAGRVWLPGERSKRWHGAYGGGVYF
                     VPFNLFIISATAGFSEKERMFNFTVGKRINARY"
     gene            51512..52168
                     /locus_tag="PLO70_02490"
     CDS             51512..52168
                     /locus_tag="PLO70_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQZ73348.1"
                     /translation="MKSIKVIPSRDRIVAVSVISSVIIFTLTFSSVFFTNQTWIDEKA
                     FQIISPFISEERTSLMKFISSLGRHSFLIPANLVLIILFAVLHKKWMAVRVAFVALTS
                     LGLMSLLKNLLQRQRPPEPLVEGITNFSFPSGHAFMGITFYGLLIWMIAIHVKNKWQK
                     GLAITFLSLLIITIGLSRVYLRVHYTTDVLAGFSIGFLWLIACLNIIEKAESGFVNKS
                     "
     gene            complement(52145..53011)
                     /locus_tag="PLO70_02495"
     CDS             complement(52145..53011)
                     /locus_tag="PLO70_02495"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HQZ73349.1"
                     /translation="MKFLNFLPHSCTAIGLLALLISSTGCQNRKPRMIKSPPHYNFGE
                     VYTHKLDIKLKEISGLAWDNKNDEFVAHYDEAGKLFYLDKETKVIKAEYTFGKKGDYE
                     DVVLINSIPYVLRSDGMITKVSKDSTGKIYGEELGKIGITGVNDFEAMYYDPTRNALV
                     LICKNCALDNKSSVSAFAYYFDSTGFDNKPVFTIDAKEVERLSPHKTSKFQPSAAAIH
                     PVLQKLFILSSASNQLAIVDLDGKVEAVFELGKKLFPQPEGLTFKSNGDMYISNEGVI
                     SKATILRFVYKP"
     gene            53058..54323
                     /locus_tag="PLO70_02500"
     CDS             53058..54323
                     /locus_tag="PLO70_02500"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HQZ73350.1"
                     /translation="MENQSTLLKDAQDAATNLISSKVSKNVRFHTLQHTREVVAACEK
                     IAETLPLSDDDKIALNIAAWFHDTGYSAGQAKDHEMISVQHAVDFLNAHATSEEMIKK
                     VTGCIIATRMPQNPTNNIEQIICDADLFHLGSDNFKEKSKLLRDELKEFGGEELSKKE
                     WRQNNIAFLEAHKYFTAFGQTNLQPVKEKNLVELVEKKPEEMAKPEKKNKDKKNKIKE
                     PELAEQAAAEAKAKKLKENQTERGISTVFRIMAQNQSNLSQMADSKANILISVNSIIL
                     TIVISTLFTKLENNSNLQIPVILLVVVCVSAIVFSILATRPNITHGTFTQEDIHNKRT
                     NLLFFGNFFKMSLPDYDWAMQEMLNDRNYLYSSIVKDNYFLGVVLAKKYKLLRIAYNI
                     FMFGLILAMIAFAIAIAIPQAGEIYTPGG"
     gene            complement(54472..56328)
                     /locus_tag="PLO70_02505"
     CDS             complement(54472..56328)
                     /locus_tag="PLO70_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQZ73351.1"
                     /translation="MARKKELLNDVVIKFAGDSGDGMQLTGSQFTNNTALLGIDLATF
                     PDFPAEIRAPQGTLPGVSGYQLRFSSDRVFTPGDACDVLVAMNAAALKVNLTALKMGG
                     KIIANTDGFDAKNLRLANYPEGENPLENGSLGNYEVIKMDVTKMTREALKDITMGMKE
                     KDRAKNMFVLGFLYWMYNRDMENTISFLKEKFGKKPDILESNIKVLQAGYNYGDTTET
                     FTTTYSVEKAKMEPGSYRSIMGNQAVAYGLIAASQKSGLPLFLGSYPITPASDILHDL
                     SKYKNFGVRTFQAEDEIAAITSAIGAAYGGALGVTTSSGPGIALKGEAMGLAVMLEIP
                     LIIVNIQRGGPSTGLPTKTEQSDLMQAYYGRNGECPMPIISASTPTDCFDAVYEAVRI
                     SVQHMTPVIFLSDGYIANGAEPWKYPQSADLPPITVKFKTELGHGEEKFQPYLRDEKL
                     ARPWAVPGTIGLEHRIGGLEKQNITGNVNYEPENHQLMVKIRQEKVDKIADYIPEQKL
                     DSGPDKGKILVIGWGSTYGAIKSAVSEIQAKGYAVSHAHLRHIRPFPKNLGDLIKNFD
                     TILIPEINNGQLIKIIRDIYMVDAKGYNKIMGVPITKTELVMKLEEMLGRAN"
     gene            56803..57378
                     /locus_tag="PLO70_02510"
     CDS             56803..57378
                     /locus_tag="PLO70_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73352.1"
                     /translation="MSSIFKQYYLKKLIIWGGIGLIALFIRYSSKPETSTLIGFVIIL
                     AFLSVGLINGFIDYRFYEKSAPRLVSNLLEQKPLLEFKAIGFSKEDDDKIGGYINNYK
                     IFLAPLINVEGEKWLTVLIPLQVREGLDNYFNKFDDFFKFQLSDKVLFAEAILKSYDK
                     TYDYQKIFTLLEKTTLSLQDKKILPINIVDE"
     gene            57624..>57946
                     /locus_tag="PLO70_02515"
     CDS             57624..>57946
                     /locus_tag="PLO70_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ73353.1"
                     /translation="MNPSSSADDGDPAKQIFLRPALSLFFVLALLTFSCNNKDTKKET
                     PALPIIDSTLVRQQQQKAADSIAKAAALKKKIYITFDDGPNKGTRNVLAAVKEENIPA
                     SFFIVG"
BASE COUNT        18015 a        12167 c        11213 g        16551 t
ORIGIN      
        1 gcatcaacat gaaactgata acaacaccat agatacgcac cggcagtgtc atatcaccca
       61 aataagggga caaccaggca atcagcactg cataatagat aaccaccagt aataatatcc
      121 atatattact ttttataacc tctgacaatc ttacatggtg aaagaatact atgtaaaaaa
      181 tatgcgccaa taaaaaggaa gataaaccca gcaaaaagaa aatatctttt tgtccctgga
      241 acatcagcaa cacatcccca ccccaggaaa aaatcaacgc caatacaatc cattttttga
      301 gcttactctt gatgctgctg gttgctgtaa taaaatagcc aattaatacc ggtacaataa
      361 gtggcttaaa aatatattga agaacttcat tgcctgtttg cataccgatg atatcagcaa
      421 tcagaacaat aataaaaaga atgatccaga agtttttttt catgctgtgc ttttatgcat
      481 ctatccaaac ttcactttcc ttcagcaaat taatcaattc atccacggcc acttcactgt
      541 tcacattctt cttcactact tctttgcctt tatataaggt tattttaccg ggaccactgc
      601 ctacataacc aaaatcggca tcggccattt caccgggacc attcacaata caccccatga
      661 tggcaatctt cactcctttc agatgattgg tgacagcccg gatctttgct gtagtttcct
      721 gcaggtcaaa taaagttctg ccacaacttg gacaactgat atactctgtt tttgagattc
      781 ttgtcctggt agcctgtaga atactgaaag ctgtattatt ggtaaattgg taaatatcct
      841 ttacctgcaa gtaagtacgg ccggttactt ttaaactttc catctgagcc ttattactgt
      901 agcccagaca aataccatca cccatgccct ccaaaaataa agccccggtt tcagtggcaa
      961 aatgtataag gtgttcatcg ggagtttgcc aattactatc cgtaatcagt actaccgggt
     1021 ttctgatact gttgttttgc agttctataa acatcctccg aacggactgc atagcattag
     1081 tattggtact gctcaggcaa ataaccactg tagggtcatt agacatttta tccaggaaag
     1141 tatagtcatt caccggggta ctatcgctgt aacaatcctg cattacaaaa ttcagtttat
     1201 cacttttaaa ttctgcctct gcaaaccccc cggcctcaaa aatgggaaaa tactttgttt
     1261 tatctgctac cagtgaccag gctccggggt aaacaatcac ttttaaagta cccggcaatt
     1321 caaaatccaa taattggtta ccagtaaaaa tataatctgc tgctgcatca ctgatattcc
     1381 atttatctgt ggcttcatta taagtatagc caacactttg taaatctttt gctagtattt
     1441 tatcaatctt actcaggtcc gcaatcacca caggaacatg tttaccgcca atattatcaa
     1501 ctgcaaatgt ttctcttctt ttgtactcga acggagaata agatatttta tttgtttcag
     1561 gaatcaattc ctggccatgg gctcccgact cccggctcgt atatcgcctg actaaatctt
     1621 tacatacggg gatttcaagt tcaggatctt ctgtcaatga aacacggatc gtatcgccca
     1681 acccatcttc gagcaaagtg ccaataccaa ttgcgctttt tattctgcca tcttccccat
     1741 cccctgcttc cgttactcct aaatgcaaag gataacattc gccaaattca tcaaccattg
     1801 tttttaccag caagcggtaa gcctgcacca ttacctgcgg gttactgctt ttcatgctca
     1861 gtacaatatt atgatatgct tccgctcttg caatgcgaag gaactccatg gcactttcca
     1921 ccattccatt tgccgtatct ccataacgac tcataatgcg gtcactcagc gatccatgat
     1981 tggtgccgat ccgcatagct gttccatact ctttacaaat cttcaccagc ggagtaaacc
     2041 tttcccgtat ccgatcaatc tcttctgcat actcagcgtt agtatactct atctgttcaa
     2101 attttttctt atcaacatag ttacccggat tcaccctaac cttctctata atcctggcag
     2161 caatttcagc agcattggga gtaaaatgta tatcagctac cagcggtgta ctgtagcccc
     2221 ttttcttcaa ttcgttcttg atattcagta aattctctgc ttcttttttt gacggggctg
     2281 tgatacggac cagttctgcg ccggcttcga tacaacggat actctgctcc accgtggcaa
     2341 tagtatccat ggtgtccgta gtggtcattg tttgtacccg gatgggattc aaattaccca
     2401 acaaaaggtc acctatttta acttctcttg tttttagacg cctgtattca gtaagcgatt
     2461 cacagtacaa ttgcatagat gcaaaactaa caaagaacct gcagcttggt ttaccaatct
     2521 ttgacctggc cacccccggt tttagatttt tccttttgta gccgctggtt tacctctttt
     2581 tctttttgct ccagcagctt aaggcgttgt tccgcttcct tgggactcat tttgggctgc
     2641 tgctgttttt tttgctgctc ttgctgtttc tttttctgat tctctttttc ttcttttttt
     2701 ttaggaggat ttttcttttt caattccaga agggccttct gaaggttttc ccgggcttct
     2761 ttgtcatctg gattttgccg gagagaattt ttatatgctt caatactttc ttccagtttc
     2821 ttctgttggg tcagcaccac tcccttatta taatatgact ttgacttcag ttccttttcc
     2881 ttggctttct ctgataattc cgagaaagac tttagtgcct catcctgctt accctgtttt
     2941 tgcagactgt tggcaagatt gaattttgtt gtagtatttt ccgggtctgc caaaagtgcc
     3001 ttgttatatt cttcggttgc tttttcaaat tgccgctgct tgtaatattc attccctttt
     3061 tgagttgcct tttcaaccac ctgtgactgt gcaaaaaaac tggctgaaac caatattact
     3121 atggcaatga attttctcat acagtcaacc ttttttttct ctccggaata aaattctcta
     3181 tcaataacaa aaaaaacata gccgctgcaa accacaggta atatgtttta aaattcatct
     3241 gcgatacatc gccaaaagct ttgcgctcta tctgggagag ctggcttttt aatactgcta
     3301 tagcttcatc actgcttttc agttgtatat acacaccatt tgtttctgtc gctatttctt
     3361 tcaactcctc ctcattcaat tttgagatca ctgtattacc agcttcgtct cttttgttca
     3421 gaccagtggc tggatcaata attggtgaac cttccaccga accaatacca attgtattga
     3481 ccataacacc ctgctcagct aacgctttgg ctgtagtaat tgcagtttcg tcatgatctt
     3541 caccatcaga aatcaataca actgctttaa atctttttac gttactgtta aaaacaccag
     3601 cactcatctt caacgcatca ctgataacgg ttccctgctg tggcactgca tccggagtgg
     3661 cggaagaaac aaagagattg gctgctccat ggtcaatagt taagggcatt tgcagataag
     3721 ccttcccggc aaacaataca agggctaccc ggtcattggg catcgctccg atcagtttat
     3781 tgatcagttg ttttgccctt tctaacctgt tgggggcaag atcggtagcc agcatacttt
     3841 tgcttacatc cagtgcgatg gctatatcaa taccttttct gctaatatta tctgcatcgc
     3901 ccggtttacg gggattcatt actgccaaaa tacccaatgt aaaagcagca acctgcaaaa
     3961 tgaattttac agtgaaacgt tccgaagaaa aattatttat taaaagcctt accagtttct
     4021 catcaccgat ccttttcagg gtatttcttt tccattgcaa caggctaaga aataaaagga
     4081 caagaacagc tatagcaacg aaaagccata caaactcttt atattgaaac tgccagctca
     4141 aaatgtattt tatgtaccct gaaaggcaaa tattgtgcta actacatcct gccttcaaaa
     4201 aaaccgagtt gcttaaggat atttttgagc attttcatcc ttatcgtcga catatcaaag
     4261 tctttatcag gagattcgat attcagcaca aaaaagtagg ggtggttgtt ttcttccacc
     4321 cagcctacca cccaaccaat ggcattaccg ttttctttaa atccccaacc ggttttataa
     4381 cccaacctgt agttggaatt attttcaaat aacatagccc tctttaccat ctcctggtaa
     4441 ctcttaaaaa atggcaattg atcaaaatat agtcttttta ccaggcccag ctgctggtca
     4501 ggtgtgatct ttaaagaatt atcaagccag aaactatcaa tggcagaggt tatcaccact
     4561 tttcctgtat cagttttggc accatatttt aagctatcca accaaatttg catggtgtct
     4621 ttaccaatcc ggcgggcaac ttcctggtaa taataaacag aagaaacccg gaacgcctca
     4681 tacatattca ggtctttgtt ccagtcagct actcttcttt caactccatc ccatttaatc
     4741 accatactat cgctgctgat cttcccggtt tgcaaaccga tgagtgaatt caaaatttta
     4801 aatgtactcg ccggcaaata actgctgtca cgataacggc ccaggttatg caccgtaaat
     4861 ttaccggaag cattatccat cagagcaaag catccctcca ctttattgtc atcaaaatat
     4921 ttcttcaggc ttttatcctg tttaacatta ttgggagagc aggattgcaa aaaaactatc
     4981 agcaaaagga aattagagaa ttgtttcatc tgggaattaa aatttcggca aaggtagcca
     5041 tcattcggtt cgctttaaaa tagctttctg ataactacca actttataac tgtcaacaga
     5101 cggactgttc tctgttccat tcccgcttgt cataataggt tttgcagaaa tgctgaaaaa
     5161 ttctatcatc agctgatcat tctctatcca gtaattgttg atgattgtag cgttcattac
     5221 cgtaaacgct ccacaaaatt tatcagcaac ccagaactgg tcaagcacaa taccattatt
     5281 ctcatcaatt acccaatgcc ctttggacgt atcttttgcg atcaatgtgt agggccggtt
     5341 atcttccttt gatgaaccat aaattatttg ccaggtaaat ttatgggcac tgtccgccgg
     5401 atgaatgcgc aattccatat tcactttttg aggttcttta ctaccggttt taaaccatag
     5461 taattctcct ttccagttac cgatccagtt ttgaggaaac ggattttcct gaccagaaat
     5521 aatatgagaa aaaaagctgc atagtacagt aatgagaagc ttcatgtgac ttgttttgct
     5581 aaaaataaaa aagtcccggc ttataaccgg gacccgaatg ttttgattat ccttaactct
     5641 ctacaggtaa ataccatttt taaggcgata atgtttatta ccctggcaat ccctttttgt
     5701 agcagcgcaa gagctgaata caaacatgac gaaggccgct gtggccaggc aaaccaataa
     5761 tttcttcata ctttttttta aaagttgggt caacaaatta attaaggaaa ttcaagggaa
     5821 ttgggcgggt agagtttact tcatcgttag taggaaacga aagtaatacc caaaaattgt
     5881 atttcataac ttatgctgct ctttttcctg ttttttatga aaactcacga agtaggttat
     5941 cctattagta gaactccgat acatagtaat tttactcgta taaagatcaa tcaattgcaa
     6001 ctcattgcat ttcacgctga atataaatta tttcaatacc cctagctctt ttccaatctt
     6061 tgtaaacgct gctatagcct tatccagatg ctgctgctca tgagccgcac tcaattgcac
     6121 ccgaatgcgg gcctgccctt ttgcaaccac cgggaagaag aacccaatta cataaatgcc
     6181 ttcttccagc aatcgagttg ccatcgtttg tgcaagcact gcatcataca acatgatggg
     6241 aacgattgga tggtcgccgg gttttatgtc gaaaccggca tcggtcattt tagtgcgaaa
     6301 gtatttggta ttgtattcca acttatctct taattcagtg gtttcggtca gcatgtccag
     6361 cacggcaata gaagcgccaa caatagaggg agccagtgta ttactgaaaa gataaggtct
     6421 tgatttctgg cgcagcatat caatgatctc tttacggccg gaagtaaaac caccggatgc
     6481 tccacctaaa gctttaccaa gtgttcctgt aataatatca attttaccca tcacaccacg
     6541 gtattcatgg gtacctcttc ctgtctttcc gagaaacccg gatgaatgac attcatcaat
     6601 cataatacag gcatcatatt tttcggccag tttcactatt tcatcaaggc gggcaatggt
     6661 accatccata ctaaacgatc catctgttac aattatacgg ctgcggagag tggcagactc
     6721 tttcagttta gactccaggt cattcatatc attatgttca taccggaaac gctgcgcctt
     6781 acacaaacgg acaccatcaa taatggaagc atggttcaat gcatctgaga tgatggcatc
     6841 ctgctcattg aagagaggct caaacactcc gccgttggca tcaaaagcgg cggcatataa
     6901 tatggtatcc tccgtaccca ggaatgtaga gatcttttct tccagttctt tatgaatatc
     6961 ctgcgtaccg caaataaaac ggacactgct catgccataa ccccgcaggt caatagcttt
     7021 atgagccgct tcaataactt tagggtggct ggacaatcca aggtagttgt tagcacaaaa
     7081 attcaaaaca gtttttccgt ttacgataat ctcagcacct tgcgggcttt caatgatcct
     7141 ttctgtttta tacaacccgg aagatttaat ctcttccact tctttagcaa tacgttgcac
     7201 cagtttctca ttcataaagc atggtttata agcaaatata agaattgaag cctctttaca
     7261 gctaccggaa aaacttcaca tgaatatgcg attgcaacca ggaaaaagaa attttacgtt
     7321 tgcggtaaca ttttgaaaac aactttcgtc acatccctaa accaacacca tgaaccggaa
     7381 taaccagatt tttttctttt ttctgaccat tttactggca gtatcccttg ttattctgcg
     7441 ttcttcctca tctgcaaatt caccgaaaga aagtaatgag caggcattac ctacctgctg
     7501 taaaaaggta aaggaaaaat gcccggagcc aaaaaaagca gttcctgcag acctgatgct
     7561 ggaaagctta tcccgtcagc ttatcactat ttctgctttt tagcattgat tatcagccca
     7621 taagatggta ttggttcagg attttgccga aagttttccg ttcctgtaac attaatgttc
     7681 cctaaaccgt attacttaac atgcaggtat caatgattat ttttgcctgc gctatcggct
     7741 gtttatgact gaaagagaat acaatgaatg tgtgaatacg tacgcagata atgtgtaccg
     7801 cttcattctg aaaaatcttc gccacgaaga agatgcccgg gatgtggtgc agaccgcttt
     7861 tgaaaaaatg tggcgtaacc gggatgatgt agatgcacaa aaaagcaaat cctacttgtt
     7921 tacggtggcg tatcaccaga tgatagacca tatccgtaaa gtgaaaagga tacaactaag
     7981 ggaggaattc agtgccgagg caagagtgga gaaccgtccg gccaataatc tgaagaagat
     8041 actggacgag gcattgaacc ggctgggtga aacacaacgt tcactggttt tgctgaagga
     8101 ctatgaagga tatagttatg aagagatagg acagatcatg aacctgaacc cgagccaggt
     8161 gaaagtttac ctgcaccggg caagggttca gctaaaagaa tatttagtga aaattgaaaa
     8221 cgtaatttag aagtacgagg tatgaagtca gaagtacgaa ttacaggagt tcttctgaca
     8281 tctgacctcc aacatcataa aacatgaata ttaaccgcca taattacgaa gagtgtttta
     8341 tcctttacat ggataatgag cttagcagcg atgaacgccg tatggtggaa gaatttattc
     8401 aacaccaccc cgacctgaaa gaagaactgg aaattttaca gcaatttaaa ttgtcccctg
     8461 atactgatat catttttgaa ggcaaggaag aattattgaa aggacaagaa aacccctctc
     8521 taaatctttc taattatgaa gaatcgctgt tgctttatac cgacaatgaa ctgaatgcag
     8581 ctcaaaaaac aatggtagag caatttgtta aagaaaaccc gtccattaaa aaagaaatgg
     8641 aattattaca acggactaaa ttccaacctg aacatattgt atttgcccac aaagaatcat
     8701 tatatagaag agaagaaaaa gtaaaggcct taccggttcg ctggtggaga gctgcagctg
     8761 ctatcctgat tctgggattg agccttacta ctgctctatt atttaataat aagaaagcac
     8821 ccgaacccgc tgaaatggca aagacaacag gtaatgaaga aaaaaatgta cctgccggtc
     8881 atcaaaacaa agagaatgct atagcagttg ttgaagaggt aaaagctccc ggtaataatc
     8941 ctgaagaaaa aaataacaca accgtatatg caccggataa caagcagtca actaatcaag
     9001 tagctgtaaa gaataacagg gctgaaaacc caataccccg taacagtata gtacctgtga
     9061 aaaataatga accattgcta gcagataatt taactggaac aaataatctt ccgaagccgg
     9121 aaagcaatcc ttacgtcaac agtgatttac caagaactga aatcgccggc aatatcccga
     9181 aagaaaaaaa tactttacac ccctcattaa caaatcctgt tgtaacaaac agaactactt
     9241 ctccgtcaga ctttgtacaa gcatccaaca caatcagtga tgaagatgta aacgatgcag
     9301 gtggcaagaa aaacaaactc cgtgggcttt tccggaaagt aacacggaca tttgaaaaaa
     9361 gaactaatat tgacccgacc gacgatgata acagattatt ggtgggcggg ctggccatca
     9421 gactgaaata accttattac aaattttaaa aacagaaagt atgaaaagga tttttaccct
     9481 atgcgtagcg ctgtgtacta tcgccagcag ttttgcccaa acagacacga ccgctacccc
     9541 ccaacctgca ccacaggatg acactatccg tataggtggc atggtcatta tacgtaaagc
     9601 cggcagcaaa gacaaagaga tcatccgcga caaggaatat aaaatgcgga accgcagaac
     9661 agacaaacca tcgaacctca gtaccaactg gtggatattt gaccttggtt tttctaatta
     9721 taatgaccaa tcaaattata cagctgctgc agtaagcgga tttgttgccc cgggtattgg
     9781 tgaagatcag ttgaaactaa gagccggaaa atccaggaac gtaaacgtat ggttctttat
     9841 gcaaaagctg aatattgcca aacatgtact gaacctgaaa tatggaatgg gaatcgagct
     9901 gaacaactat catttcgaaa atgataaaat agtattcgct aaaaacccaa cttatatttc
     9961 tcagggcaca acagaattta aaaaggtaaa actggcagca gattacctga cagtgcctat
    10021 gatgctcaat atcaatttca cccctaaaag aaagaatgga tttggcctca gcggtggtat
    10081 cagtgccggt tacttatatt ctgcccgcta taaaacaaaa gacggcaaag atgtggaaaa
    10141 agtgaaaagt gattttgacc tggaacgttt caaattatct tatataggtg aattgtcgct
    10201 tggcccagta agattatatg gcagctatgc catgaagaat atgtgggaga agggattgga
    10261 catgactcct tatacactgg gtttccggtt tagcaactgg taatacaaca gtttctcata
    10321 ttcatacatg aagccggtcc tgtttccagg accgcttttt tgttggtaaa aagaaaagat
    10381 gctgggaaaa cctccccgta tcttttctaa agagtccgtt aaaacatatc cacagcccaa
    10441 aataaaaaaa catggcattg cgtttgagta attggacgga cacttttact gaaaaagcca
    10501 tgaataaacg cagcacccac cttagcctgc aatataaacc tggccttctt gaaaacatta
    10561 catggacatg aaaaacttat ttattctcac atctgttttc ctgggaagca gcctgctggc
    10621 tactgatgta catacaggta aaagaaccgc aatcaataaa aggttcagtg gaacgcttta
    10681 caaaggcccg gtaggaacca tttcaatcat tattgataaa tcagattatg aattgaatgt
    10741 atatgacgat aaaggctggt atgccactta tcccgtagtt tttggaaaca gcagccttgc
    10801 tgataaaaag atggaaggag ataaaaatac tccggaaggc acttaccgca tcacggccaa
    10861 aagggtgcat gaaaaatggt gccgctttct ttctattgac taccccaccg ctgcagaccg
    10921 tgcaaaattt aatcagcgta agcaacgggg cgaaattccg gccaatgccc gtattggtgg
    10981 cggtataggt attcatggca cctggcccca tgaaggtttt gtgatagacc gctataaaaa
    11041 ctggaccctt ggttgtattt ccatgaaaaa tgaggacgta acagaaatat accaatttgc
    11101 cggcgccggt accacagtaa caattaaaaa ataattgcta ttggctattt tttccgagaa
    11161 aatttcctac ctccatccgc cattttacag gatcattctg caataacgat tcatgatctg
    11221 cattctcgta accaactaat ttcttatcag atgaaaaaat agccgtaaag actttatcaa
    11281 tttcgctttt caatacataa ctgtcttttg ccccgtactg aaccaggacc ggacaggaaa
    11341 ttttttttgc ataccggtct gttgtatacc tgtacccatt gaatcctctt tcaataccca
    11401 tccagccggt aactaaaaag gcaaatggct gttgtggaaa acctaatact cttgcccttg
    11461 ctttcaggag tgattgtaaa gaggaaaatg gcaattccag gatcacagca gccggatata
    11521 ttgaatactc ggacaatgct ttggcaataa caactgcacc catagagatt ccccataaaa
    11581 aaatcttctt ttcccctttt ccctttacaa aatcataggc cagtttcact tcttcggttt
    11641 ctcgtatccc aatagttgtc gtggagccat cgctattccc atgtgctctg aaatcaacca
    11701 gcatcacatt ataacccagg tatcggaatt cattagcctc tgtaagcagc tgtcccttat
    11761 tggaagtaat accatgaaat agtaatattg ttccttttgc tacagaatca gttttagaat
    11821 accatgcatc aatggttagt ccattttcgg tttgaaggct aaccgtatca taaataaaaa
    11881 caggtacatc atttatttgc gactttggaa attttgggcc ggtaaaaagt ttccatgttt
    11941 ttgtaaaaat attcccggaa ggctggctgg ttcggagtga aggatcatta taaaaatggg
    12001 tgagcctgta tgcataaaaa gctgcgctga tattgatcag tacaatctga accaatatta
    12061 cccaaagtgt ccaacgaagt atggatggcc acttttgtct ggtttttttt tcttccataa
    12121 gatattattc atccggcagt ccggctttcg gtcaaaagta atttttagaa tgcagacctt
    12181 ctactgtcat gtctcttttt ttgagatgat tgtgcaaaac attctaataa aaaattcaga
    12241 atggaatatt tattgttatt tactttgtgt cacacttgcc aaaaataatt atcgggaaaa
    12301 ccttttaaag cagctcatcc gtgctgcttt aacttttttt acaacttccc tttctttatt
    12361 tcctctacca cacccgggtc aagcagcgta gttatgtcac ccagattttc tgtttctcct
    12421 tccgcaattt tccggagaat acgtcgcatg atctttccgc ttctggtttt cgggagacct
    12481 gttacaaact gaattttatc aggcttggca atcggcccga tgacacgggt aactgtttgc
    12541 agaatatcct gccgtgtgag gttttcgtcc acatgcatat gttcgtagat gacaaatgca
    12601 tatacgccct gcccctttat atcatgcgga tagcctacta ctgcactttc caccacaccg
    12661 gcatgcatat taatggcatt ctccacttca gcagtgccga gtctgtgtcc actaacattt
    12721 aatacatcat ctacccgtcc ggtgattctg taatttcctt cttcatcctt cagggcacca
    12781 tcaccggtaa agtacaggtt ttcataagcg gcaaaatagt tggtcctgca acgttcatga
    12841 tcaccatacg ttgttcttat tattcccggc catggtgctt taatacaaag gtttcctttc
    12901 agcaatccct gtccgtcctt ttcagtcact tcttttccgt tttcgtcaac caacactggt
    12961 tgcacacccg gcatgggtaa agtagcccaa cttggtttcg aaggggtcac attcgccagg
    13021 ttggaaatta aacaaccact ggtttctgtt tgccaccagg tatcaacaat aggacatttc
    13081 cttttaccga tattatcatc ataccaatgc catgcttctt cattgatagg ttcgccaacg
    13141 gtaccaagta ttttcagaga agttaaatct tttcccttca gaggttcaag gccaaatccc
    13201 atcaggcttc tgatagctgt cggtgctgta taaagaatat tcactttatg cctgtcaaca
    13261 atatcccaga atcttccggc atctggccag gtagggattc cttcaaacat tactgaagtg
    13321 gctcctgcac ttagcggacc atacaagata taactatgac cggtgatcca gccaatatct
    13381 gctgtacaga aatgaatatc gccggggtta tattgaaaca cattgacaaa agtataagtg
    13441 gtccatacca tataaccagc cgtactatgc accactcctt ttggtttacc ggtgctgccg
    13501 gatgtatgta aaataaataa aggatcttct gcatccatct cttccgccgg aaatgacacc
    13561 ttaccgtttt tctccacttg tgcttctgca tgctccattt catcttccca ccacacatct
    13621 cttcccttca gcatggagac aggagttctt gttcttgtat aaacaattac tttttttact
    13681 gttcggtttc caatcaaggc atcatctatc acacttttca acggaatatc tttaccaccc
    13741 cggtatgcac cgtcacaagt cactataaat tctgcctggg catcatacaa cctgtccgct
    13801 atactttggg cagaaaatcc tccgaatatt acactatgaa ttgcccctac tcttgcacag
    13861 cccaatacag cataagccag ttcgggtacc atgcccatat agatacaaac cctgtcccct
    13921 ttcttaacgc cattattgat caacacctgt gcaaactggc aaacccgttt gtgcaaacgg
    13981 ttataggtaa cgattctcac cctttcttca ggattattag gttcccagat aatagcaggt
    14041 ttatcaccca tcgtttccag gtgtcggtca atacaattct ccgtgatgtt cagttttgcc
    14101 cctttgaacc actccacttt gggctcttta aaattccact ccagcacttt atcccacttt
    14161 ttcctccact gaaaattttc agcaaccgta gcccagaaac cctccgggtc ttctacgctt
    14221 tttttatatg tttcatgata ctgttctaat gattttatct ggtagggata tgacatgatt
    14281 ggcaaaaatt ttagtgtttt ttctaatgag tgataaattt acataatgac aggcaaaaca
    14341 gccattattc atcgggaacg tttccggtga aattgttttt tacatttttc attaatactt
    14401 atcttacccg gatgaaacca actacgatcc ggagtgtaat tgcagcttct tctgtaggaa
    14461 cgatgattga atggtatgat ttctacatat tcggcatgct tgccaaaaca atttctaccc
    14521 agtttttccc ggagggaaat gcgacagctg ccttattaag taccctggca atttttgctg
    14581 ccggctttat tgttcgtccc ttcggagcac tggtttttgg ccgcttaggc gacctgatag
    14641 gcagaaaata tacattcctg cttaccctgg tattaatggg tggttctact tttttgatcg
    14701 ggcttgttcc cggttataaa tccattggta ttgctgcgcc tttactggtt ttattattac
    14761 gattgataca aggtcttgca ctgggtggcg aatatggcgg agcagctacc tatgtagcag
    14821 aacatgcacc ggcaaacaaa agaggttttt ataccagttg gatacaaacc actgcgacat
    14881 taggattatt tgtagcactg ggaatgatca tgctggtcaa gctgaatatg tcggaatctt
    14941 cattcaatgc cgaatggggt ggctggcgtt atcctttctg gatttcaata ttgcttgtgg
    15001 ctgtttccgt ttatatccgg ttaaaaatgt ctgaatcccc attgttctcc aaactgaaag
    15061 aggaaggaaa aacatcagtc aaccctctaa aggaaagttt tagtcataag gccaatttca
    15121 aaatggtact catggctttg tttggagccg taatgggaca aggcgtggtt tggtataccg
    15181 gccagtttta tgcacagaat tttttagaaa ctaaggctat gcttgatttt gagcaaagca
    15241 gaactatcct gctttgggca atcgcttttg caacaccctt ctttgttttc tggggatggc
    15301 tgagtgataa aatcggaagg aaatggatca tgatggcggg gatggcattg gcaatattta
    15361 cttacagacc gatatttaat acgttcctgg ctgatgcaga tcttccagcg gcaataataa
    15421 aaaaacagtt gcccccggaa actgtaaaaa tagaacctgt aataattaaa gcagatacag
    15481 taaaaagaga agctggagtt atcattatca atcatatcca ttcaaagcat acattaccgg
    15541 atggaagtag attctccgct tcgtatacag attcaatttt taataattcc aatggagtta
    15601 aaaatgaagt gaaaacaagt cctattacct atgtcaacaa acatttaccc tcgtccttgt
    15661 actggaaatt cattggactg gtttttcttt tgatcttata tgttacaatg gtctatggac
    15721 ctatagcagc tttcctggtg gaaatgttcc ccactaagat ccgttatacc tccatgagcc
    15781 ttccctatca tatcggcaat ggtgtttttg gtggcctggt tccgtttatt ggtttgttgt
    15841 tgaccacctc gtttccttcg gatccgttgg tgggattgtg gtacccaatt ggtgtggcag
    15901 cactttgcct gctaattggt atagtatacc ttagaaataa aattgacgaa aacgtagaag
    15961 attaaactca ttcatatgaa caacataaaa agagcattgg gcattatttg gctattattg
    16021 gctccggcgg ttatttattt cctggtggac agggctattg tccatattga tccggccggc
    16081 acaaaagata tcaacaaccc ggtgatatgg atcattatca tcggtatttt cacacctatc
    16141 gcaataggtc ttatgatttt cggctggtat gcttttaaag gagaatatga tcacctaccg
    16201 gaatcttcgg aagagctttc ctgattaacc gttttactat tgatgctttt aagcattaat
    16261 agtcccgcta tagcagccac cagggaagca ccaagaatag caatctttga attcactact
    16321 gtttcattat ctgcaaaagc aagtaatgta ataaaaatgg acatcgtgaa cccgataccg
    16381 gcaagcagcc ctgctgccgc aatatgcttc caggtaaccg aagatggcaa actactgaat
    16441 tttgttttta cggccaacca ggcaaatgtt agaattccca atggcttacc aattacaaga
    16501 ccggacataa taccaagact attgacagag accagggtat tgtaaaaacc gggttgaagt
    16561 tttatacccg tattggctaa tgcaaaaagc gggacaataa aaaaagctac cgggtaatgc
    16621 aaaatatgtt gcagtttaaa cgacggtgaa tgagcacccc cttttccaaa aggaatggcg
    16681 aatgccagta atactccgct gatcgttgca tgtacacctg actgatacat aaaataccac
    16741 atggcgatac cgggtattat ataaaacagc agattcttta cgcctgtttt acccatcata
    16801 aataaaacaa caaatattcc caatgaaata aataaattaa ggacagacaa atcttccgta
    16861 tagaaaatag caataaccat tattgcaccg aggtcatcaa taatagccag tgctgtaaga
    16921 aagatcttca gggcagcggg aactgatttc ccggccagtg aaagaatacc taatgcaaaa
    16981 gcaatatcgg tagccattgg tataccaaat cctgttgcag tggcagttcc aaaattaaaa
    17041 atagcatgga tagctgcagg gaccaacata cctccggcag cggccactat aggaaaaatg
    17101 gcgttcctga gctgtgacag ttcaccaaca taaatttcac gttcaatttc caatcccacc
    17161 agcaggaaaa agacagccat tagggcatca ttgatccaat gcgaaatact aagcccgcct
    17221 atcctggtat gccaaaactg atcatatccc tcgccccatg gagaattggc aatgaccaat
    17281 gacagtaccg tacagcctat cagtataaaa cctgcagctt tctcactatt aaaaaattcg
    17341 gaaaacagcc tggatagccc aaaatcattt aagtccttct ctttggtctt tacttccata
    17401 tcaaaaatga tttacttcaa agcagcaaca aaggtcatag tttagaacta aatggacgag
    17461 ggttttatca ggctccttct gcactgttta ccagaaaaat tttacaattc tcaaacaaat
    17521 tgcacttgtc tgtgttaata tctgactata tttttgcatc ctctaaaatg aagctagttg
    17581 cagttatatt attttttgtt tttgccggta ctgtatttgc accggtagca caatcactct
    17641 gcaaagacag tcaaattagc ctttttatag tggatgagga aaaaagcaat actcaaattg
    17701 ctgaaaatga agacactaaa aaagaacaat ccaaaacaat cttttaccag ttgattttag
    17761 cttcaataag tgaaaaaggg gatgcggctt ttctaaatag cgcagatctt cccaattctc
    17821 cttaccggga ttattctaca cctccccctg atttcgtttg atgaaccccg caacggcata
    17881 gccgtgtatt ctttcattaa tctttcgtaa taatttatca tttcatggaa aaatctttga
    17941 gtcctgttgc caggatatgg aacctggtgc gccaggaaaa aactgaaata acagccattt
    18001 atttttatgc aatcctggct ggtttaattc aactttcact acctgtaggt gtacaggcca
    18061 taataagttt tgtgttggga ggagtaatgt ctacctcgct gatattactg ataacattag
    18121 tagtaactgg agtacttgca accgggctta tgtatataaa ccagatgaag ctgattgaaa
    18181 agatccaaca gaaaatattt gtccgttata cagcagagat tgccgaccgt attccgcggc
    18241 ttgacctgaa aaaaatcgat tctttttatc tgcctgaact ggtgaaccgg ttttttgaga
    18301 tccctgtatt gcaaaaaggg ctgtccaaat tattactgga tataccaaca gcaactattc
    18361 aaatcctgtt tggattattg ttattatcat tttaccaccc cgtatttata cttttcggca
    18421 ttttattaat cctggtactg gcaataatcc tgtactatac cgggtcaaaa gggttaaaga
    18481 ccagtcttgc tgagagcggc tataaatatg cagtcgccgc atggctggaa gaactggcga
    18541 gagttgtaaa atcttttaaa ctttcaaaag gcacttcatt gcatttgaaa agaacagatg
    18601 aacgaactat cacttatctc aatacaagaa cccagcattt cggcgtactg ctctttcaat
    18661 acagaacact ggtcgcatta aaaacaataa taacagctgc catgttactg gtaggatcat
    18721 tcctcctggt aaatcagcaa ttgaatatcg ggcagtttat tgccgcagag atcgttattt
    18781 taattgtgat cagcgctgta gaaaagatca tcggcaccct ggatagtgta tacgatgtac
    18841 ttacttctgt ggagaagatc ggaaaactca ctgacaaacc cctcgaacaa cacggcaacc
    18901 acctgcttcc acaaaaaaaa gaaggtgtat cgattgaatt tgcagctgtt caatttggat
    18961 tttcaaacaa cagtcacatc atcgatgatc tgaatttcag aattgaagcg ggtgaaacgg
    19021 catgcatatc cggaaaagaa ggaagtggca aatccatatt acttcatctg ctgggaggtg
    19081 tatatactga ttttaacgga tcaatactgg tggacgaaat tcccatcaat aattataata
    19141 tagattcttt gcggtcacaa acgggtattt acctgagcca ccaggatatt tttcatggct
    19201 ctctgctgga aaatattacg atgggaaata ataatattgg tatggatgaa attagccggt
    19261 gggttgaaaa acttggacta aaatcattca tctcttccct gaaatatggt tttgacactc
    19321 aattagaccc tcttggtgat cacttgcccg gtaacgtagt aagaaaaatt cttttactca
    19381 gggctttgat caataagccc cgattaatac tgctggaaga gccctggctt ggtttcactg
    19441 atgaagtaaa aaataaaatt caggaggcat tgatcaatga tacagccggc agtacagtaa
    19501 tggtgatcag taatgatgaa tcatttgcct caaaatgtag taaaagaata caactgaaca
    19561 gacaataaaa aacaaataat ggaaagtaaa aattacgata taaaggctta ctcacatata
    19621 tacaggtata acagcagaag ccgggttaaa tactggctct atggcttgat catctttaac
    19681 acagcactcc ttttccttcc ctggacccag aatatccgtg caaaaggaaa tgtgacaaca
    19741 ttacggcagg aacagcggcc acaacaaata aataccatca ttccgggaaa agtggtcaag
    19801 tggtatgtaa aagaaggtga ctttgttaac cagggtgata cgattctgca actggcagaa
    19861 attaaggatg aatatcttga tcctaacctc atcagcagaa ccggggatca gctttctgcc
    19921 aaagaaatga aggtagagta ttataaaagt aaagccggta ccgccagtac tcagattaat
    19981 gcgcttcaaa atgagcaaca gctaaagctg gaacaaaccc gtaataaaat aacacagcag
    20041 gaaagaaaga ttgagagtga taatctcgac ttcatggctg cggttaatga actttctgtt
    20101 gccggcagac aactggcagc tggcaaggca atgcttgact cgggtgtgat ctctttaatt
    20161 gaatatgaaa aacggaaagt aacatatcag aatgcacagg caaaaaaaac aggagcagaa
    20221 aataaacttg ctaatgcaaa acaggaactt gctatcctga atattgagtt aaacgcaatt
    20281 tcgcaggaat ttacagagaa aatttctaaa gcatcaggcg accggtttca gtctatgtct
    20341 gaagtagcca ccgggcaggg cgatattgcc aagctacaaa atcaatttac caactataat
    20401 atacgtagcg gtatgtacta tgttactgcc ccgcaaagcg ggcaggtaag taaagccaaa
    20461 aaagcaggac tgggtgaaat acttaaggat ggcgaaatga tcgttgagat cgttccggat
    20521 aagattcaat ttgccgtcga aatttttgta cgaccggttg atctgcccct aataaacccg
    20581 ggacagaaag ttcgtttttg gtttgatggc tttccggcta ttgttttcag cggatggcct
    20641 aacgcctctt acggaacttt tggcggaaaa gtatttgccg ttgaaaattc agtaagcagt
    20701 aacggcaaat tcagaatact ggttgcagag gaccctgctg aaaggccctg gcccaaagag
    20761 ttacgtatcg gtgcaggtgc ccagggtatg gctttattaa aagatgtacc ggtatggtat
    20821 gaattgtggc ggaatataaa tggatttccg ccagactatt acaaagttga caaaactgaa
    20881 aagaagtaat aagtatcgat caaaaaagct aaaaaatgaa aacacaaaaa aaatatatac
    20941 ttctttactg gataaaaaaa agaagagaaa aaagaaatac aattattaaa acaaccatca
    21001 taggtataac attaatgctg ctcgcactgg tggcagctgc acaggatacc ataaagacat
    21061 tatcaccaca gcaactggct gccataatac gtctttatca cccggtagcc cgccaggcgg
    21121 atatcaatat agaaaaggcc aaggcaggta tcatcaccgc aaaaggaggc tttgatcctc
    21181 tcctgaccaa tgctacagcc caaaaaacat ttgatggcaa taactattac tattataacc
    21241 gtcctgaaat tactatccct acatggtatg gaatcagtat taacggcggg ttagagtatt
    21301 tatccgggaa ccggaccgat ccccgggata caaaaggaga atcaagcttt attggcatta
    21361 ccataccgct tgcaaagaat ttattgatgg ataaacgaag ggctgctttg caaactgcaa
    21421 aaataatgaa agaggcatct gctgttgaaa agagaagtat aattaataca cttatgcagg
    21481 aagcactaac tatgtactgg aactgggtga agcactacct gatctataaa cttgcaagtg
    21541 atgctatagc tatcaatgaa aaaaggattg aactgatccg gatctcattt cggcagggag
    21601 acaggcctgc acttgacacc atcgaagcct taacacaatt acagaatttt cagctatggc
    21661 aatcacaagc caggcttgac ttccagaata tgggactgga attatccaat tatttatggg
    21721 ctgcagataa tactcctttc tttttacaac cagatgtgat accggacgag gaatcacaaa
    21781 tgctaaaggc tgatagcttc gtaacaccgg atcttgcaca aattctcgaa accatcaggg
    21841 atcagcatcc tgacttgtta cagtatcaat tcaaactaag tgcacttgat attgaaaaga
    21901 aattaaaatt ccaggaaata ttacccaccg ttaactttcg ctataatcaa ttgggcagag
    21961 ggtatgacat tgccaaaacg ataacaaacc cattatttga aaatagtttt caatacggta
    22021 tcagcattgg tataccctta cggttgtctg ccggaagagg tgaatataaa acagcaaaat
    22081 tgaaaataac tgaaacaaaa ctacaacaat atcaaaaaat acaacagctg gaaaataaaa
    22141 taaagagcta ttataatgag ctcgctgctc ttaaaacaca ggtttcgcta tatgaaaagg
    22201 ctgttcaaaa ttataacacc ctgctaaaag gcgaagaaac ccgtttacta aacggggaaa
    22261 gcagcttatt tcttgttaat aatcgtgaaa gtaaaaccct ggaagcactt caaaaactaa
    22321 cagaattaaa agtcaaatac ttccaggcca atagcctgtt actcggtaca gccggaatgc
    22381 tcaactaaac gataaagcag aacaggggta atcaataatc agtatggaga atcatccgca
    22441 aattcaggat gacctgaaag ctaccggatg attcaatttt ttatgcggcc cgaaaaagac
    22501 ccgcaataaa atacgaaaag ccgccagttc aatgaataca aaacatagtg ccataagaat
    22561 gatcaaagca acctggttgc tgtgcacata agtaaaaagt acatcaatac ctacaaatgc
    22621 tgctgttaac ggaaaaccca gtatacccat agctgatagc aaaaaaagaa agccggttgt
    22681 ttcctcctca tagacatacc catgaaactt gtttagcgaa atatcgttat caatagtctt
    22741 gattttgtgc aaacaataat accccagcat gaatgctgcc aaaaccccgc tggcataaaa
    22801 aataatttca ataaaattta catgcggtac attaaacaat attgcagcga tgataaataa
    22861 atgcccggtt aataaatata tccatgcttt caaagcagat tttctgtatg aaaatgaaaa
    22921 aagtataact gacagggcaa tactcattaa agtaagcgga agtataccac caaaggtttg
    22981 tacaacggtc ggactgaaat aacccagtgc caaaacgcca agccccgtga acagaagtac
    23041 agcaacgaaa acagttgagt gtaaaaattg aaactttttg ccgatccatt taaaaggact
    23101 ccacagaaag ttaaacataa tggtatctaa attccactcc tttataccca gtatatatag
    23161 tgaagctttg atcctggaca ctggcttttg catgggtaga gtataatgaa aatactggtg
    23221 atgtaccaaa taatttaaca ccgaaggcga aaccagcagc tgatacgttc ttaaaaatgc
    23281 attgcctgca aaatgaatga gcacaagcca atgaaagcct aatgcaattt caatgaacat
    23341 taatccgatt tgtgcagctg aggaataagc gatctgcgtc tttaccgttg gctgtactct
    23401 ggcgatcatt gttgcaacga cagctgttag cccgccaata ataatgatta aaacctttgc
    23461 ccataacata tcctcccaaa aggggtgcgt acgcagcaat aaaaaaatac cgatatgaac
    23521 actcaatgaa ccgtaaaaga tagcagaaga agaagtagga ccctccatgg ccctgggcaa
    23581 ccaggtagtg aaaggaaatt gcgctgactt tatagccgca ggcaaaatca acatacaaac
    23641 aataaagata gccatcccgg gttgatccaa tgctatggct gtatttttgg cttcacccag
    23701 ttgggcgaat gtaatgtttt gatgtgtgag gtgatgcatc atccacatgg ccagcataat
    23761 agccacgtca ctgatccggt aataagaaac agccttgaat gcatttttaa caggcaaata
    23821 acggttgcgg taaaaagcta ttaataaaaa tgagcaaatg ccttttatct cccagccaat
    23881 aaaaagagtt tcaaaatttc ctgataaaat aataaaatta taagcagtag caaataacaa
    23941 aatagtatta aaaaatcttt tatacccctg gtcacggtgc atataatatt tgctgaaagt
    24001 tgctaccaaa aaaaataaca gcgcccctac tacactaaat acagctgtta tttcatcata
    24061 atacaattga attgcaaata caaaatcatc tgtttggtaa agggttgcca gtttatagct
    24121 gacaggttta tagccttcca acatccacca aataaaaaag gatatggaga caactatata
    24181 aaaaatctta gtgacccgta cgatatagcc gatgggcctt tcacttttgt tttgccatac
    24241 aagtgaagca aaaaaagcca gcatcggaat agcaatgaat aagatcagaa gttggttcat
    24301 ttatattttt ttcatacagg ttttacagaa aggaaataat caaattcata aaaccggtat
    24361 gctcggtgag ctgttttaat ccagtaaata cactggtaaa ttttcctcgg tagcatgcac
    24421 aatatgattc gtttccattt cctttgcccc ttcaataaat tcttccatat tgtgaatggt
    24481 tttaatatcc cctgcatcgg taatgggttc atattttgta aatgcaccgt ttctgaaata
    24541 ataaaactgc ttttcttccg ggtgaagggc cacaatatgc acccactcgt tgttatacca
    24601 ttcaaatact tcaggggagg actgtatagc tttcaaaacg atatcaggcc ggtgctccac
    24661 tatcaccaat aaccttaccg gatcatgcac ttcgatcatt tgccacggaa ggccgggtcg
    24721 cagatctcca tcactactat tagccactcc aaacagtccc atcacattat gaggaagctt
    24781 ggtacctgca cccagcttta tattatctac tcttgaaaaa taatactcga gattgatgcc
    24841 accacaaacc aacccaatgg gacgcattac agcagccagt atagtcccgt ctgcatctgt
    24901 agtatgatca taactattga gaaaagcacg acggtccaaa aacaaacctt tggttacctg
    24961 cctgcggcca attatggcta atgtatttgt accatgaccc agttccggcc ttggctcaaa
    25021 caaagagaca gaacggctat gaatcgcttt acgaacctgc tctaactcct gtttggtatt
    25081 gatagatgca aatcgcctgc ttcgttcttt tgcattcagg ttaagtgctg tttcaaaatt
    25141 ctgaatattg ataatgtgtc ttttagtatt ttcttctgaa agtatctttt catcataata
    25201 ggccatcaca tctgcagctg tatcatgcat ggcaccaaca aattgtgtgg aagcgggaat
    25261 gtttattccc ttacctgaca atatctccct tattttagta tgatttaata taaatgcctg
    25321 taccctggcg ttcgtcgcac cgggtcttcc gctgcaggct ccgcaatcat gtgcaccgtg
    25381 atgcgggttg ttggcgctgc tgcttccgtg tgcaacaatg taaacgattg gtgcaaaatc
    25441 tttgagtaag ccaatacctc ttaagaaacc ttcgcccctt gctgccatct cttcaagcgt
    25501 ataaccgacc tgcaatccat tctcaatatc tgatagatgc ttattttcaa tagtgagtga
    25561 cgattgttta tccatgtgtc cgtaagcatt ggaaatagcc ggactcattt tcggacgaaa
    25621 catcatttgc atcttcttta caaatgccca taatccaaag cctatgctca ggaaaaatcc
    25681 tgataaaatg ccatgtgtgt atttcgtata gatcagttct tctttttgga gtgattttga
    25741 atcagactcc tttataaggt atttgggtgt tactggtgcc gggcacagct tatcataaaa
    25801 tttactcccc tgctgctgaa aatagaactc cacaccaaaa aaacctggtg cgccaaatgt
    25861 ttctgcgctt ttatctactg cctctatatg tcgccggatg gaatcctctc tttcatcgat
    25921 gcagaaaata gcctgaaggg aggggccgac agttttatcc gttaatgaga atttatttag
    25981 ccccttatca tttaaaattc ttttcgaatc agcaagacta atacctttca gcactgaatc
    26041 ataataactc cattcaaaag cgtcctgcca tagttctatt acctgggcaa attctgtttt
    26101 gtgcacctca gcaaagaaat ttaccggtgg tgctgaaaca tggctggcca aaggctgcca
    26161 tttattccct aatgcgtggt ttaatgcatc cagttccatc agcaattcaa attccaccag
    26221 ctctttgaat gtgatatttt ttctgtccaa cagcgtatgc gggttatctt ccaccgcact
    26281 tacaaaacca ctccatccat gatgggcaaa ttgctgatca aataaatact gttcgaaata
    26341 tgctgaatca ccaacaataa cttgtaacaa atctgtaatg gtatactgcc cggataaaaa
    26401 ttgctgcctg acagccttgg tcctgaagaa actgacaaaa ctattctgct ccaattcttt
    26461 tatactgtca atgaaagatt tattgacaac gggaaaccct tcaatagcaa taccctggtc
    26521 caggaaagta caaagtatac gaaacagcag ggggtgtact ttattatcta aatccagccc
    26581 atatacatcc ttccaatgtt ttcgcaactg cccgattcgg ggctgattga ttgtatcata
    26641 ctctttactg atcaagtttt tcttccaatc agctacagcg tttttccctt ttttaccagc
    26701 gatcaccatt tgcagaatat cttccctgat tcgtccggtt ttatacattt cccggtattc
    26761 cggcaattgc aaatgcacct ggaagccaaa aattttagag gctgtaaaga tggcatcata
    26821 aaacttcatg tgctgaaacg catgcaggga attatgatga ataaaatccc gcaatgcctg
    26881 ttgcgatgga agataatgct tcaattcatg gaggacatgt gcttcgttaa aggaatggtt
    26941 atccatataa aatagggttg gagattattt ccaacccagt ttaaaaatta atgtcttaat
    27001 tcggccacac taaattttgg atttttttca tcctttatat aagatacatc tttataaaac
    27061 tccaccttac cggtatctat attatacatg gctcccacaa tgcctatttc tccttttttc
    27121 aacatttcct ccaggataaa gctgcgttca ataatattct tcacacttcg tttcacattc
    27181 aacgtagcta cattttcaac aaattttcca tttttcgaat ttctcttatc cgcagccgta
    27241 gtttcagatt cggagtaaac tgctggttgc agtttggata ataattccgt aaggttgccc
    27301 atttccacat gatcgcaggc tccttttaca gccccgcatt tggtatgccc aagcacaacg
    27361 atcagctttg acccggctac tttacaggca aattccatac tgcccaatat gtctgtgttc
    27421 acaatatttc cagcaattct gatagagaaa atatcaccca atccctggtc gaaaactaac
    27481 tcagcagaag tgcggctgtc aatacagctc agtatggtgg caaatggcca ttgaccttcc
    27541 ctggtatcat ttacctgttc aagcaagtcc ctttgggctt tcaggttttt aataaaccgc
    27601 ccgtttcctt cctgtaatat ttccattgcc atcctgggcg ttaaactggc ttgtgtttct
    27661 tttgaatgtg ctctcatttt aaatttttct tttactgttg tataataaat atttttaagc
    27721 cggctcaata aagtttattg tttcaaccgc tatatttttt gtttttgcat tgcttttgaa
    27781 atcacggatg agttccacca catcataagc tatggattta gaattgctgc aatcaatgat
    27841 aacttttgaa ttttccggaa tctcttccaa ctcttttatc acactggctt tattaaaaaa
    27901 ggaaacttct tcggcaagca ccaggtgatg tacttcatgc ccttcttctg atgtaaccgt
    27961 ttctttcatg taatgcgaat ttctgaagct gtgacgtagg gtgtaaaaaa taccgaacag
    28021 catcccgatc gtaatccctt tcagcaagtc tgtaagcaaa ataccaataa ctgtcgctac
    28081 aaagggaata aactgctccc aacccaattt gtacattttc ttaaacagtt caggcttcgc
    28141 caatttatat cctaccatta ataaaacggc tgctaaactg gcaagcggaa tcatattcaa
    28201 aacactggca attgtaatag cacttatcaa taaaaatatc ccgtgaagaa tggcagatag
    28261 ttttgttttg ccaccaaatg taatattggc tgagctgcgc actattacct gtgtaacggg
    28321 taaacctcca attaatcctg aaaatatgtt tcccagtccc tgggctttta gttccctgtt
    28381 ggtaggggta attcttttat tggggtccat tttgtctgtt gcttcaacac tcagcaaggt
    28441 ttccaggctg gcaactatag ctaatacgat ggcaatcttc cacacttcaa cattagtaat
    28501 tgctgaaaaa tcgggaagag tgaactggtt aaaaaattca gttaaattgt tggcaaccgg
    28561 cagcctaact acctggtcat cagctaaact aaaatccagt gtcccagatt taaagagata
    28621 gttcattaca ataccaagaa ttactacaac gatggggcct tgtatcaact gaaaaatttt
    28681 atgctttttt gttaacaccg aatcccacaa aatcaataaa cctaaagaga tcgctgcaat
    28741 caaaacagca ccgggtgtaa taaaatccag agcctgtgca atctcggaaa aagtattttg
    28801 cccgtcggcc tgtaaaaaag catcatcgcc ttctgcgtcc ttatcccagc ctaatgcatg
    28861 tggaatttgt tttaatataa tgagaagccc tattccggta agcatccctt taattaccga
    28921 ggaaggaaaa aagtaggcaa taaagccggc tttggcaaag cccagtgcaa gttgaataaa
    28981 gccggctaat acaaccgcta gtaaaaaagc aggccaggat cccagtgtag cgatagaggt
    29041 taatacaatt actgccaggc cggcagcagg gccgctaacc cccaaagaag aaccgcttgc
    29101 aatacctaca ataataccac ccacaattcc tgcaatgatg cccgaaaaaa gtggcgcccc
    29161 ggaagccagt gcaataccca aacataaagg aactgcaaca aaaaatacta caatacttgc
    29221 aggcaggtca cttttaattt ctttaaacat actattgttc ttcatgagaa gctattttga
    29281 ttcgattaac aatgaaattt atcgctacca tcataaatga tgataaggtt gctttataat
    29341 aattaagaaa atgacacaag acggcgtctt ctagtaaaat agaaaaaaac tacagctggt
    29401 tgggtggggg gacaagtaat tcgccgtgga aagcggtata cgtacctgag ttctcgcatt
    29461 tatggtattg cgaaacaatt aataaaaaat gatcagcccc gtgatgatca tgcaggtatt
    29521 cttcagaaaa agaactgctg ttgggcgttt tttcttcagt agtattgcca aagggattgg
    29581 ctgtttcttc ttcggtgccg gcattggcag acaattgctg gctacttcgt tctttctggc
    29641 tattaaacac aaaaggagta cttacagtaa gccataaaag agccaatatc ataaatatgg
    29701 ccgaagccac ctggaaagta ttatatgttt tactgttttt tcccatcaaa gtgtcgcaaa
    29761 ataacatttt ggaccttgta atccaaacct ttctacaagg ccgggtgaat gtcaaaattt
    29821 cagattgcct ttattaaccc ggtaaaaata acaggtaagc agtaaacttg tttaagttta
    29881 ttgtcatatt ttatttaatt ttttaaataa ggcgcaaaag ttagttttca ttttgtatgt
    29941 attagaggct atttttaaat aatatttaaa ctccatatga aaccatacga ggttttatta
    30001 aatgataata aacattgggc agccaaaaaa ctggctgaag accctgattt tttcgaacgg
    30061 ctattacatg tgcagtcgcc agaattttta tggatcggct gcagtgacag cagggtgccc
    30121 cccgatcaga taacacaaac tgaaccggga gaaatcttca ttcatcgtaa tgtggctaac
    30181 ctggtagtaa acacagatgt taatttaatg agtgtggtgg attatgcggt caaccatttg
    30241 aaaataaaac atgtggtggt ttgtgggcac tatggatgtg gtggcattaa ggccgctatg
    30301 tcaaacgaag actttaacca ggtgcttaat atgtggctcc ggaacattaa agacatttac
    30361 cgcatacacc ggaaagaact ggaaaatatt cctgatgaag aaaaaagggt taaccgtatg
    30421 gtggaattaa atgtaaaaga acaagtattc aacctggcta aaactgctct tattcaaagg
    30481 gcctggagca gtgaacagag acccgattta catggctggg tgtatgacct gcatgatgga
    30541 ttaataaaac cgattgtgga aatgaaagca ggtaccgata ttgaccccct ttacgaattt
    30601 gaagatttat aatagttgct cagctgtgaa ataaatacag ttgcaaaaga taaacccctg
    30661 cccctgccag gtaacccagt aatgccagcc agccaatttt tttcatatac cagaaaaatt
    30721 caattttttc caaccccata gcagctaccc ctgctgcaga gccaatgacc aggatacttc
    30781 cgccggttcc ggcacaataa gcaaggaatt cccagaaata atgatcggta gggaactgtt
    30841 ccaggctata cataccctgc gcagctgcca ccagtggtac gttgtctaca attgctgaaa
    30901 gcaggccgat caatacggtg attattttaa tattacctat tgagttgttc attgaaaccg
    30961 caagatcagc cagtactccc gttacctgta aagctgctat actcagcaaa atgcccagga
    31021 aaaacaaaat acttggtgta tcgattttgc gcaatgcatg attaacagaa taaaggcctt
    31081 tttctgcttc atccttccca ctgtgtatca tttcagttat cacccacata acccctaaac
    31141 ccaataaaac acccatatag ggaggcaggt gtgttatggt tttaaataca ggaacaaaga
    31201 gcaagcttcc cagtcccagc cagaatactg tcttccgctc tctgctgttg gtagtaaaat
    31261 ctttattttt tattggttca actgattgac cgatatgacc ttttaattgc ctgcttatta
    31321 taatagcggg tactaaaaag caaacaagac ttggcagtaa cgttttaata ataatattac
    31381 ctgctgtaat ctgacctccg atccacagca tagtggttgt tacgtctcca atcgggctcc
    31441 acgcaccacc tgcattcgca gagataatca ccattcccgc aaataacagc ctgtcttttt
    31501 tatcaggaat tattatacgt aataaagaaa ccaacacaat ggtagtagta aggttatcta
    31561 agactgccga aagaaagaaa gttattaatc caattaccca aatcagtttt cttttattag
    31621 tggtcctgat gcggctggta attacctcaa acccatcgtg tgcgtcaatc agctctacaa
    31681 tggtcatcgc acccatcaaa aagaataaaa tacctgctac ttcacccagg tactctgtaa
    31741 gctgatgggc gggagcatgt tcattgtcag aaaacaatgc aaatacagtc cagcacaaaa
    31801 cccctgtaat tagtgctgtg gctgctttgt taactttaag gggatgctcc aatgctattg
    31861 ccgcataccc cgctataaaa atgatggaaa taacggtaat cataagccat aaaaataatg
    31921 aaataagttt caggcaaccc cagatcaact aatttaaggt tttgatgtag gtttgtagta
    31981 tgtccatcga agtaaaaaac cttctcaaac aatacggcga gcaaaaagcg gtcaataata
    32041 tttcatttaa agtagacaaa ggagagatag ttggtttcct ggggccaaac ggcgcaggaa
    32101 aatccacaac catgaaaatt ataactggtt atttggcgca aaccagtggc gaagcctatg
    32161 tatgtggtat taatgtagcc gaagaaccac tggaaactaa aaagaagatc gggtacctgc
    32221 cagaactaaa tgctttgtac tatgatatgt atgtaaagga gtacctggcg tttgttgccg
    32281 aactgcataa aattgatgat aaacggaaag cggttgaaaa agtaattgaa acagtgggcc
    32341 tgactattga ggcaaagaaa aaaatcgggc aactgtcaaa aggatataaa caaagagtag
    32401 gcctagcagc tgccctgatc catggacctg aagtgctgat acttgatgaa ccaacatctg
    32461 gcctggaccc gaaccaaata gttgaaattc gagaagtgat aaaacaacag ggaaaagaca
    32521 agaccatttt attctcctca catattctgc aggaagttga agccatctgc gacagggtca
    32581 ttattattaa caagggaatc ctcgtggctg atgataaaat gagtaaactc agcaaaactt
    32641 cttccaatat tgtgaaagtc acattcagag agagtataga tattcagctt ttggaaaaat
    32701 tgcctgctgt taccgggttg acaaaaaccg atacgaacaa ctggcagtta gccacacatg
    32761 attctgatca gttacgcaaa caaatattgg agttggcttt gcggcacaac ctcaacatcg
    32821 tttctttgca aagcgaaaac caaagtttgg aagatgtgtt cagatcatta acacagtcac
    32881 atacaattca atcttaaaaa caagcaatta aatatgagct acatttcaga aatcttcgcc
    32941 agacaaattt tagattcaag aggtaatccc actgtagagg tagatgtatt aaccgatgaa
    33001 ggcgccctgg gccgtgctgc tgtaccaagc ggagccagca ctggtataca tgaagcagta
    33061 gagctaagag ataacgataa gaaaaaatat ggtggtaagg gtgtacttaa agcagtagct
    33121 aatgtcaata aaacaatcgc accggcatta ctgggttatg atgtggccga ccagaccggt
    33181 attgacgaaa tgatgattca actggatggc actcccaata aagggaaact gggtgccaac
    33241 gcaattcttg cagtaagcat ggctgctgcg aaagctgcag ctgaagaagc ttcattaccg
    33301 ctgtaccgtt atatcggtgg tacaaatgca aaagtacttc ctgttcccat gatgaatatc
    33361 ctgaatggcg gtgcccatgc cgataataaa atagatttcc aggaatttat ggtcatgccg
    33421 gttggtgctc caagctttaa tgaaggttta cgctgggggg ttgagatatt tcatgccctc
    33481 aaaactgtgt tgaagaaaaa aggatatagt actaatgtgg gtgatgaagg tggctttgca
    33541 ccggatattc aaagtaatga agaagccatt gatactgtaa tgctggctat aaaaactgcc
    33601 ggcttcaaag cgggtacaga agtggcaatt gccatggacc cggcggtaag tgaaatgtat
    33661 gatagtgcca aaaaagtcta tcacttccat aaatctgatg gcaaaaaact gaccagcgaa
    33721 aaaatggtag actattgggc taactgggca aaaaattatc ctattgtttc tatcgaagat
    33781 ggcatggctg aagatgactg gagtggctgg aaattattaa ctgataagtt gggtaaaaaa
    33841 atacagcttg ttggggatga tctttttgta accaatgtaa ttcgtttaca aaaaggtatc
    33901 gaaacaggag taggtaatgc tttacttgta aaagtgaacc agattggtac cataaccgaa
    33961 acaattaatg ccgttacact ggcacagcac aatggctata atactatcat gagccatcgc
    34021 agcggtgaaa cggaagacac taccattgct gacttagctg ttgcgctgaa ttgcggacaa
    34081 ataaaaacag gtagtgcctc aaggactgat agaattgcca agtacaacca actggtaaga
    34141 attgaagaat tgctgggaag ctccgctgtt tatccaaaag gaaagattaa atttggtaaa
    34201 tagaaatccg aagtcggggt cagaaacctg aatgacttta tttcgggttt ctgactacag
    34261 actaattatc tccgatatta tgaaactact aacccatatt ccctcctgga tcaaaagcaa
    34321 atattttatt gcgtttgcag ctttttgcgt tgttgtcctt tttttagata aaaatgattt
    34381 ctttacgcag attgaccgtc aaaaagagct ccgggaactg caacagagca agcaatacta
    34441 ttccactcaa atatctgcag aacgcaaaga attagaggca cttaagaata atccggctac
    34501 tcttgaaaaa tatgcacggg agaagtacct gatgaaaagg gataatgagg aactcttcct
    34561 ggtatcggaa aagcccgata attcaaagaa ttaagaatta ctgcacaata aggcaatccc
    34621 gtccccgtta ataatccgga cattcgtaat atttcagtta ccaacggaca acatcttatt
    34681 tctggacaaa ttaatcggtt tgccaaatga caaattttac acccgaggac cttttattgt
    34741 atttgtataa cgaaacaact tctaaacaga ctgctgccat tgaaaaagca cttgaaaaag
    34801 actggacact ccgtgaaaag ctaagtgttc tgaaagtttc gatgcagcgc cttgacaaga
    34861 tcactgaatc accccgtact gaagtagtac tgaatgtact gaattatgca cgggaacaag
    34921 ttgtacagga aatacaatag tgctttcagc agttcgaaaa ttattttagc attcgccata
    34981 gcgaatgctt tttttattgt aaccaccgga gctttttgca aaggagattt atctttcccc
    35041 tttatcttgc agtatgaccc gtaaagaaaa ataccagttt gtcattgact atttccagca
    35101 acatgcacct gaagcagaaa cagaattact ttatgatact ccctaccagc ttttagtagc
    35161 tgtaattctt tccgcccagt gcactgacaa aagggtaaat atgaccactc cggctatatt
    35221 taaaaaatac ccgacagtag acgcattatc gaaagcaaca tttgatgagc tgttcccgct
    35281 tattaaaagt atttcctatc cgaataataa aacaaaacat cttattggga tggctgttaa
    35341 agttctcggg tcttttcatg gaaaaattcc gatgacagta gatgagctgg tacaattacc
    35401 aggtgtggga agaaaaacgg ccaatgtgat tacatctgtt atcgatcagc aaccaaatat
    35461 ggcggttgac acccatgtat tcagggtgag taaacgtatt ggcctggtac cgcaaacagc
    35521 ctctaccccg tttgccgtag aaaaaatact ggtaaaaaac ataccggaaa aattgataca
    35581 taaagctcat cattggctta ttcttcatgg acgttatgtt tgcctggcaa ggaatccgca
    35641 atgtgaaaaa tgtgggttgc aacaggtttg cagctattat ctaagggaaa aacgctgagt
    35701 aataattcat acgtttcagg taaaaactta tttgataaat ttaattttta gcataatagt
    35761 tgcaacaatt ggccgatcat tcacactatg aaaggccaac ttgtgaaaaa catataccag
    35821 cttcttctct tgttattacc ggtaatacct gtgtacttga aagcacagat tcctccccgg
    35881 caatgggcac agcattatgg cggtgccaat gttgatattc cctatactat aaaatttact
    35941 gccgatggcg gaactgttgt tgcaggatac actgattcaa aaagcggaga tgtaagtgca
    36001 cagcccaacc gtgaatactg ggatctgtgg gtattgaaat tagataagtg cggcaccata
    36061 caatgggaaa aatcatttgg cggaaggggc tatgaaagtg caagagatat agtccaaaca
    36121 gcggatggag gatatatggt gctgggtgaa accaattcta cagatggtgg tgttatagcc
    36181 ggatacggtg gaacaaaaga tatctggctg ctgaaattat cagcttcagg aaatcttgaa
    36241 tggcagaaaa ggtatggtgg aagcggactt gatataggta accatattga attaactact
    36301 gatggcagtt tcttaatcgc agccacctcc tcttccaatg atggagatat aaggggcaat
    36361 cacagcaccg gtaccttcac ggatggtgtg ctaatgaaaa taaatgcagc cggaaatctg
    36421 ttatggagca aatgctttgg cggcagcaaa aatgaagaac tctttgatat tgaagtcatc
    36481 aatggaacaa tcttcgcagt tggttttacc aattcaattg atggtgacat acctcctgat
    36541 cagaaaaatt atgacatgtg gttgctggct attgatgccg gcggcaataa ggtactcagc
    36601 aaaatttatg gcggttccca gaatgatgtg gcgtattgca tgaccaaagg agctaatggt
    36661 tcattaacac tggcaggtta tactacctca tcagatggtg atgtaagcgg agcaaaaggc
    36721 agccaggatt attgggtcat aaatctggac caaaaaggaa aactgaactg gcagaaggta
    36781 ctggggggca cagatgcaga atatgctaaa acaattctca ctgatagcga tagtagctat
    36841 atcataggtg gtgtcagtta ctctaacgat ggagatataa acggcgcact cggtgagggc
    36901 gacttctgga cagttaaact cagccccaca ggtaacgagg tttggaaaaa gaactggggc
    36961 ggcaatagga atgatcattt acgttttatg ataagaaacc cggcaagaaa tgaatattac
    37021 ttagcgggtg attccgaatc aggtgatgga gatttcaata atacaattgg tgatgcagat
    37081 tttgcaatta taaaattaaa gataccggaa ctgcttactc aggattcagc tgtttgcaac
    37141 atcaatgggt tcatcccttt cgaagataca ttatatgata tttgcggttt tgactctgct
    37201 attgtgaaat ataatccagt agtattaaac gggcctttcg aaagtataag gaaatctgat
    37261 accgtatttg caggacaaag cgttactctt catgcaaacg gcaatggcat tattagctgg
    37321 aacactcatt catcactaag ctgtactgat tgttcaaatc ctgttgcaac accacaagtc
    37381 acaactgtct atactgccac gaaccgttct cccgatggtt gccaggtttc cgatcagttt
    37441 acggtggttg tgcttaatga tgctttagtt aatattccca ccggtttcac ccccaatgct
    37501 gatgggcgca atgatcattt tggaccattg ggaaaggtac ccgacgggta tcgtttacag
    37561 atatataacc gcaacggaga agttgttttc aaaagctcag caattaacca gcgatgggac
    37621 ggcaggtata aaggaatgtt acaaccaacc ggtgttttta tctacctggt ggattataaa
    37681 gacttgcaga ataaaccaca tcagcaaaaa ggaacatttg tgttgatccg ataagaactt
    37741 gttacaataa aattttactt atggacagaa gagacttttt caaacggacc aggagaaaat
    37801 attccaccac atccaatgca aatcaatacg gtaaagagat gtttgccggc cttaacccat
    37861 atacgggcaa ctggacagta aatgaagtaa cacacctgct taagcggaca atgtttggtg
    37921 ccaggaaaac tgatattgac tattttctct cattatctcc cggagcagca gttgatgaat
    37981 tactaaatac tgtgatggtt ccggatccgc cggtaagaga ctatggccta ctggaagatg
    38041 atggtgtttt ttatgatgac ctcggagtgg caatcgggca aacctgggtg aatgatctta
    38101 atatggccag tgctccggaa atcaggggcc agatcaatag ccgccgtgct agtagcctga
    38161 aaaaatggtg ggcaggactt attattaacc aacacagaag tatacaggaa aaaatggtgc
    38221 tgttctggca tcatcatttc tctatacagg aatcagaagt cgacaatgca cagatacttt
    38281 accggcatca tcatttactc cgtagtaatg cgctgggaaa ttttaagtca ctggttaagg
    38341 aagtaacaat tgaccctgcc atgttaattc acctgaatgg ttacctcaat tcaagacagg
    38401 cacctgatga aaactatgca agagaattac aggaattatt ttccattgga aaaggagatg
    38461 attcgcatta tacagaagat gacgttattg ctgctgcaag agtacttacc ggctggcgta
    38521 taaatgataa cccgcttggt tcttatcttg atgccggtgc acacgatacc ggccccaaaa
    38581 ctttttcttc attttacaac aacaccacaa tcagcggaag taccgacccc gaccaggaac
    38641 tggatgcact gatagatatg atctttacaa caacagaagc agcaaggttc atctgccgga
    38701 agctatataa atggtttgtg tattatgaca ttgatgaaac aaccgaaaca agtgttatta
    38761 tccctatggc tgaaatatta caaagtaata actacgatgt aaaaccggcc ctcgctgtcc
    38821 tgtttaaaag tgaacatttt tatgacgtat tgaaccaggc ctgttatatc aaaaatcctt
    38881 atgatatgat tgtgggtacc ctgcgtgaat tcaatgtgag ttttccttca tataccgatt
    38941 acataacagg ttatccatta ttctactcca tttaccagaa agcagcagaa atgcagcaac
    39001 aattgtttca acctcctgat gtttcaggct ggccatctta ttaccaggag ccgatgcatt
    39061 atgaattatg ggtgaacagt aattcattgc caaaaagagc tgatttcact gatgcccttg
    39121 taaatgattc ggtcattgat gtaagagcat ttgccaatta ctcaagcaac ccggcagatc
    39181 ccaaccaatt gataaacgat gtgacagcgt tgctgcttcg ttatccttta tccaatgatt
    39241 ccagggtcta tatcaaaact cattttttaa ctaacaatac gactgacgat acaatatgga
    39301 ccaatgcatg gaacagcaac aacaccacag taatcaatac atctttaaga gagctgttta
    39361 aattcctgat gaacctgccg gagtttcatc tatgctgaag tcctcctgcc ggaaacagga
    39421 tttatttaac ctatgtaata gcaataatat gaacagaaga aaatttttac gcaatacagt
    39481 acccgtggcc gttaccatgc cggcactgtt aaatggattt tcatttactg cacaaggagc
    39541 agagagtggc ttagcaagac tgctggaaca gaccctgact gatacagacc atgtttttgt
    39601 gctggtgcag ttagccggcg gtaatgatgg gttaaatatg gtgataccat tagacgttta
    39661 tggcaactat tataatgcaa ggacgaatgt ggcgattccg caaagccagg tattgcgcct
    39721 ggatggtact gataaatcag gtttacatcc tgctatgaca gccatgcaga atatgttcaa
    39781 tgatggtaaa atgagtattg tacaatcagt tggatatgca aacccaaact tctcgcattt
    39841 tagagcaaca gatatttgga acagtggtga tccaagagaa aacagaagag aaagagtaaa
    39901 aacgggttgg atgggccgtt acctcgaaag tgaatatccc ggttatcctg acgcatatcc
    39961 tgatgcaaca atgccagacc ctttagctat acaaataagc aatttcccga cattgactgt
    40021 acagggttct gtttactcaa tgggattatc aattactaat cctgaaaaag tttatacgtt
    40081 tgataatcct ttttctgatt atccccttaa tgcagctcct gcaaataaag aattaaaatt
    40141 tctccgtgtt atttcagaaa aaacaaagat ctattctgat ataatcagga ctgcatacca
    40201 gagggtggct acgttggcag catacccctc tgaaaatgta ctggccgatc aattaaaaat
    40261 cgtagcccgg ttaataaaag gtgggttgaa aacaagagtg tacaccgtaa caatgggcgg
    40321 ttttgatacc cataaaagac aagtaaatgc cagtgatacg accacaggtt ttcatgccag
    40381 gctaatgaag gatctttcgg cgggcatcag tgcttttcaa caagacctgg aactgatggg
    40441 attggaagaa agagtaatgg gaatgacttt ctcagaattt ggccgccgca taaaatccaa
    40501 tgcgagtttg ggcactgacc atggtgctgc agcaccgctg atcatatttg gaaaacatgc
    40561 caaaaaagga atacttggta acagtcctga tatacctgct gatatacagg ttgtaaataa
    40621 tatcccattc caatatgatt tcaggagtgt atatgcttcg attctggaac aatggttctg
    40681 tgtaaaacaa cctgcattga attctataat gttacagaat taccaatccc ttcccctgat
    40741 taccggcccc tgtggtgttg cagatgaccc ggatgatact aataataact cgaacaattt
    40801 gttattgaaa atgtggccca atccttataa tgtaagtgcg actattcagt tttccacaat
    40861 aggcggacat acgatgatac agcagattga tgcgttaggc agggttatta aagtactcac
    40921 caaccaggaa tatgctgccg gcacttacaa tgttgacatt tataatgacg gactggcttc
    40981 cggagtatac ttcctgcggc tgcagaataa atcccttcaa aaagtgatta gtgtgatgaa
    41041 gttgccataa aatgcttaaa atcatttaaa tatctttcac gaaatgatca ccatttagcc
    41101 agttaattga aaatccatcc atatccagga tgaacctaaa ttaatctttg tcaatcctta
    41161 taattcaata aatttacttc cgccttatcc taccctcaag cactaataat ccaatcctct
    41221 gacaaatctt tttttaatta aaaaagaaaa ttttgtttga ggatgaaaaa acttctcata
    41281 ttagggttgg ccttttgttt tgtacaggac caaccagtaa aagcgcaata ttattattac
    41341 aatgatagat attatgataa tgatgtcgta atggagatcg gtgccacttt tggcattatg
    41401 aacgccttca ccgacctggg tgggaaaaaa ggaattggta aaagttttat caaagatctt
    41461 aactggaaaa attcaaaagc aagctatggc ctttatgcag ctgccatgta caaaaatgtg
    41521 ctgggactcc gtgtagaggc aacatttgga caggtggccg cttatgacag cattttgaaa
    41581 gatgtaaaag caaccacttt tggccggtat gaacgtaacc tgagtttcaa aagcaggatt
    41641 gctgacatac aactggggat tgaggtacat cccctgtttt tcaagaatta ttatgaagga
    41701 gatgaagacc cgccttttat atcaccttat gtggtagcag gtattggtta tttttcattt
    41761 gacccccagg cgaagctgaa tggccagtgg tatgcgttac aaccactgag tacagaaggg
    41821 cagggatttg cagaataccc cgatagaaaa ccctataaac tcagtcaact aaatatagca
    41881 gccggactgg gtgttaaata tgaagtaaac tcgtttataa atgcccggct ggagattaac
    41941 catcgttttt tgaataccga ctatcttgat gatgtaagtg aagacagtta tatagacccg
    42001 gctttattta ataattacct gtcatcgaac agggcagcga tcgctcaaca attatttgac
    42061 agaagggctg aagtagatcc cggacacaca actaatccca gctatcagcg gggtaacccc
    42121 aaagacaaag atgcattctt tacgatccag ctaaaaatag gtattaacat tgggcggcaa
    42181 agacgttagc ttattttagt tattacttta gtgtaacctg taatcaaact acttgactat
    42241 aactacttgt tataaaaaac gttaccgtca ttaatactca ttcattacca atgtatatga
    42301 attctgaaaa tagctattgg cggccagtct tatttattaa ctttgataat cgctattata
    42361 gtcccccaga aaatctccgt tgcccttaat atcaactaaa ctgtaagttc atggacgaca
    42421 caaatacttt tataaaagat cctgtattca atagccttct ttctttcagg ccattggtaa
    42481 atgcacttaa aaaaaatatt gccgaaggta accccggtat gaaaaaactg tatggccatg
    42541 tggttcagga atttgaatct caccctgaat taatgggaac tataacagat cttgacgttt
    42601 tacaacctta cagcgaatta attgaagaac tcttatcagc tgtctttcct cctacaacgg
    42661 caaactatat gtacggcatt tcctttcctt ttaagaacct tgccgtctat gcgtcaccgc
    42721 tgtttaaaac gctattggtg aaaaaggata gtgctgaaat aattatacag gatgattcga
    42781 ccagcacact taaccgtgaa agattacatt ttgcctttgg attaattctg aaaaaatacc
    42841 ttggctacaa cagtgctgat tcctctaaag tagtttatcc gtttactgat gagaaaaccg
    42901 ggctggtaag atatatggag atgcgccttg atggacgctt tattgatgta agaccggtgg
    42961 atgaaatgcc aaaaatgcca gagagtattt ttgattacac cagtaaccgt ttgctgacaa
    43021 ttgaagaact gatgcagcag gtatccctgg ataaatttgt attcgaaggg ttaacggtta
    43081 tccgcattaa tgatgtaaca gaacaggctg taatagctga aatgaaaaac aaattgctgg
    43141 attctaattt tgtatcagat aacacagcct atacagacct ggaaaaacaa attcaaaacc
    43201 tgattggtct taaagacctt accattggcc tgaccccgtt ctttaaaata aacggccact
    43261 acgtttattc aaacctgcat aataataaca gccttctttt tcgccatctg cagagcacag
    43321 gagaaaaaga tgaaataagt gattattgta aattactatt ccggtacaat aatcaaccgg
    43381 ttttatacga atcattaact gaagacattc tcggggaagt acaatgtctt ccctattacc
    43441 acaaagcagg aagccgctgc atgatcatct gtccccttaa acaacagaat gaattactgg
    43501 gtatgttgga aatcagctca aaacaacccg gctatctgaa acctgaacat attagcaagt
    43561 tagaagcagc catacccatg tttacattgg gattggaaaa aagcctggaa caattaaata
    43621 accaggttga taaggttatc aagaaaaaat ttacggctgt tcagcctgct gttgagtgga
    43681 aatttactga agcttccctc aattatattg taaatatgta tgaaaaacaa gattcaggaa
    43741 ttgaacggat tgttttcgat gatgttttcc cactttatgg tgctattgat gttcgtaatt
    43801 cttcaactgc caggtcacat tctgttcagc ttgatatgct ggaacaactg gagttggcga
    43861 ggaaagttgt cagaaaagca caagctgcaa tgtcatttcc tttgttgcag gaaattgaat
    43921 ttaaaattga caggcatatt gcatccgcct ctgatgtttt gcaatcagaa gaagaaatga
    43981 gtgtctacga ttttttacag ggacaggtca tttctgtttt taaccacctg cgggatacgg
    44041 agccagccgt aaaaaatgaa atagaagaat atttttcatc actggatccc cagttgggta
    44101 tgctgtacaa gcatcgtaaa gaatatgaag acagcatttc attgatcaat catacactgg
    44161 cccgtttcat tgacaaagaa caggcagccg cccaaaaagt gtatccccac tatttcgaac
    44221 ggtatattac tgacggactg gagttcaata ttttcatggg ccagtcaata agtccacgta
    44281 aaaaatttga tgaaatatac ctgagaaata tgaagatgtg gcagttaact gttcttacaa
    44341 aggcagcaaa agtaacgcat aagctggaac aggaactgag ccaggtatta cgtactacac
    44401 aactcatcct tgcacacagt cagccacttt ccatcagctt ccgtacagaa gaaagaaaat
    44461 ttgatgtaga tggcgcctat aatacccgct atgagattgt gaaaaaaaga attgataagg
    44521 tacgcattaa agaaacaaac gaacgactga cacagcctgg gaaaatcgcc attgtatact
    44581 cacaggcaaa ggatgccgca gaatacacag agtatattga atttctgcaa agcaaaaatt
    44641 taattaaacc cgggatcgaa aagcatgatt tagaggaact gcagggtgta tccggactta
    44701 aagcactacg ggttgatgta gactttgata cagaacccag gatggaaaag aaaattgaat
    44761 tgtcagccac aacctccgat caactattga gataatactt taataatgaa gcggcctgtc
    44821 agttaaacag gccgcttcat tatttcaaac cttctataat taaaatcgcc agccaagcgt
    44881 aaccaatggc aatttatctt ctttagacat ggcataaaca aaagagaata ataaacgacg
    44941 taagggagat acccaaaagc caagaccata tcctgaagcc catttatcaa ctaaatcatt
    45001 ttccacccat accctgccaa catcataata cgctgcaata ccaatggatc cagggaaaag
    45061 ataggttctg aaatcagcaa tcttcaaacg cagctcagac tggttaaaga atttagattt
    45121 acctgcaaac cgatactgac gatagcccct gaggttatct tcgttaccaa ggtactgtgc
    45181 ctggaaaaat tcaaatccat cacccagatt gataccgccg cctacccggt tggcaaaaac
    45241 gagtcgacct ggcgccaaca ggttgaagta aaaggcaaaa ctgctgttta attgtgttac
    45301 gttatcataa ctgttatcat taagcccgtc caggtaacga actgtactta tccagttcac
    45361 accactttca ggaaggactt tattattcct ggtatcaatt atcaatgaaa ataatccacc
    45421 taagtaggat tgcctgttaa aaactttaga aggatcgagg ccactggcta ccgtatttaa
    45481 aacaatattt ctttgctgat taaacttatc atctgcttct aaatgataaa attgaaaggt
    45541 gggcccgaat aaaaatgcta ctttatcaga aaaccggtga cgaaactgaa tattgaaatc
    45601 accgagatcg tacctgatac gataaaacct gaatctgtct ggctgcgttt tatcatacac
    45661 tgaattcata ccatagccaa agaagttggt agtagtgaag gggcctttat aatcaaaatc
    45721 catcaccagg tcagtattct ttcccattac atcaatgaac tcattattgt aacgaagatt
    45781 gacggctttg gtggaaaaag catacttacc cgaaaactgg tgcagcgact tatagggtga
    45841 tttcctgaaa ccatgacgaa tgatcttcat agttggcccg agtgataacc catcatccgg
    45901 attgtacccg atagttccaa aaaaagactg gaatggatat ttgtaataaa ttctctcaaa
    45961 agaatttaca gtgctgtcat tagacatttt atttttgaac gggcccataa ctttattgtc
    46021 cccgtttaat ttatcataca acagcgcacc gcgttgggat tttgaagtat tctcaaaaac
    46081 atcactaccg ccaccaccaa tcaggcgcaa cttaatctta tcattactac cacgaagtaa
    46141 atacttatcg tctccatcca ttgcatatag acgtatctct ttggtaacgg caggatcgaa
    46201 ctttctttca tacatcttaa ccgattgttg cccttcccgg ttaattttat aaacctggac
    46261 cagtaccgac ccatcatcat tccgggaaat atcaaacagt tcttttttat cacttccggt
    46321 cacattcact atttctgcaa taaaccgatg atactctaca gcttcagcaa ctagaaaatt
    46381 gcgacgatct tttagtgtct gtgcaatttt tggagcggaa atacttctta tttctgccgg
    46441 ctgttgtgcc atagccctgt caatgacctc atctgtcatt tgagatacaa atttttcagc
    46501 ggcctgcttc cattgttctt cagttaactg gttcaggaaa aaacgatcca ggtttcttgc
    46561 agcaaaattg aatctgccaa tattattggc ttcgctttta aacccttcca gttgtggtac
    46621 catagaacgg ctttttatca aacctggcaa caaaccctgg ttaatataaa atgcctggtc
    46681 acggtctttg gctatcggga aaaactgttt cccctttcca tcatctatgt cattaacacc
    46741 ccaaagccat tgtccttcat gcctgtcaag atccataaca aacatatcca gcagccggat
    46801 ttttaaaagg gcaagctggt ccacggtgtt gtcattatcc ttttccagtt tttctgctac
    46861 ttcatcggta tcaaatactt ttttaatacc tgccggttct ctttcttcca gggtagccag
    46921 catgtttgca aactccatac ggttttcccc taatcgtgga tcatccggta tataaacgag
    46981 ttttactttc ccatacggaa taccggctgc ttcagccagc cgaattaccg ataatgaagc
    47041 ataaggataa gaagcggaga cgccgtcaga aacaagatca gcagcggcct cactctgaag
    47101 atcaccaggt aatgttttat cagttataaa tttttgaatg gtacgtaatg tatactgttt
    47161 gccattcggg tcttcgagcc gcaaagactt ggtttgttta cccccgcctc gcttcagtgg
    47221 ggtcaaccct ccactttcag tggcaagatt taagacaggc gcttttacag gtgtattcca
    47281 ttctttccgg tagttggcac ccatccagaa tattcgtcct ccgctggtgc tgaacgatga
    47341 tggctttgct gaggccgagt cagtgctttt ttgtgccgca cagtaattta atggcacgac
    47401 aaagcatgca agaattagca gtttcgttgg tctcatgatt gcagtttttg ttggttgatt
    47461 aagggcgcaa aactttgggt cctaatccta aaagatacga ttttttgaaa attgcttaaa
    47521 aatattttaa agagagggag attaatagca tagtctggaa tgtttgaaag cggaaatgcc
    47581 aaaaaaatat cagttaaaca gcttttttac tgattcagtg cctttcaggg caaagttttc
    47641 agtaaattga aaactttatt tgaacaggtt acgttatatc ccagtagtta aggcaaatta
    47701 cgctggtatt aaagtattac aaatacagta attagcaaca aattttatag agtgtatcgg
    47761 gtgtgtacaa tgaaatatat tggattattc ggcttggttc ttatactgaa cctgtcgata
    47821 aatgcgcagt cggatactat tacccaacga atagtactaa ttggtgatgc aggccaattg
    47881 accaatggca gacacccggt agtggatgcg gtaaagaaaa atattccgct tgacagcaaa
    47941 acgaccgtgc tatttctcgg tgacaacctt tataaatcag gattacccga tgaccagtat
    48001 gccctgcaat atttgaaggc taaagccgta cttgactcac aaatttcaat tgccgatgga
    48061 acacctgcac aagtgtatat gatacccggt aaccacgact gggataatgg acgggccggt
    48121 ggcttcgatg ccataatcag gcaacaattg tatgttgatt ttttacaaaa acccaatgta
    48181 aagtattatc caaaggatgg atgtcccggt ccggaagaaa tagacctggg tgaggacata
    48241 acacttatat tgtttgacag ccagtggtgg ctacacccac atgataaacc agaaattgag
    48301 tcggactgct cctgcaaaac aaaagaagag cttgttacac agattggtga cattgcagcc
    48361 cgtaatgcaa aaaaattaat cattgtggct tgccaccatc cgtttgaaag caatggcatg
    48421 catggcgggt tttataccct caagcaacat atctttccgc ttacagatat cagaaagaat
    48481 ctttatatac ctcttcccat cattggctcc atctatccta ttgcaagaag tgttttcgga
    48541 acaccccagg ataccaaaca tcctaactat gccaatatga tcgagcaggt gtctgatgct
    48601 gtcaaatcat cctcaccaaa cgttgtattt gtttccggac atgatcacaa tcttcaactg
    48661 atcagggaaa ataattataa ctatatagtc agtggcggag gttgcaagca aaaccgtacc
    48721 tctaataata ctaacagtct ttttaattcc acctccgaag gcttttgtgt aatggaagta
    48781 tcagctaata aaaatgtatc tgttgcattt tatactgtcg gtgattctgt tacacaggcg
    48841 tacaacaagc cgcttttaaa tttttcatct actgctgata cggcatatac aacatcaaat
    48901 cttaacaatc ctgatcttaa caaagacagt ataaccaaag cttcaagtaa aaactatccg
    48961 cttgcaaaag gatttaaaaa attcattatt ggtcaaaatt atcgtcctga atggtctgcc
    49021 ccggtcaata tgaaagtgtt caaaatcgcc gaggagaaaa atggtttaac cgtaataagt
    49081 ttaggagggg gcaatcaaac acaatcactt cgcctgaaag ataagaacgg taaagaatgg
    49141 gtgcttcgta ctgttgacaa gatggccaca aaagccattc ccaggaacct gagaggaacc
    49201 tttgctgaag atattgctct tgaattaaat tcagcctctc acccctatgc cccgctggcg
    49261 attcccggtt tggcagatgc cttagatatt gcggctcctc accccgaact tttctttgtt
    49321 cccgatgacc cgtcccttgg attttacagg aagatctttg caaataaaat atgcatgctc
    49381 gaagaaagac atgctaccaa tgacactctc gagatgaaaa tggtgaagcg cttttttagc
    49441 cagctaccca aagaaattca cctttcaaaa gacacttccg aaacaaaaac caccactaaa
    49501 tttttcgaca agatgctgga ggaaaatgat caccggccca tccagcagga agtattaaag
    49561 gcaagactac tggatattgt tgtcggcgat tttgaccgcc accttgatca atggagatgg
    49621 ggaagtatag ataccggaaa gggcaagatc tattacccta taccccgtga ccgtgaccag
    49681 gcatttttta actcggatgg ctttttatta aaagcattaa gccgcagagc cctgcctttc
    49741 ctgaaaggat tcaggtatga tattccaaaa gtaaattggc ttgggtatgg ggctaaagat
    49801 tttgaccggt tgttccttac cgacctggat gatactgaat gggaaaaaac aatcgcagaa
    49861 gttcagcaaa aactcactga ttctgttatc cgtaaagctg tttggaacat gccgcctgaa
    49921 atctcccgca tcagcggtga gaccattgcc aggaaaatga tgagtcgcag ggatattctt
    49981 gcaacagaag caatgaatta ttacaggttt atttccagga aagtgaatat actgggaagc
    50041 aacaaaaaag aatatttcaa aatcagcaaa tacggagatg gtctgcaggt aaaagtattt
    50101 gcaaggacaa agaaaaatga taccagtttt gtcatgtata gcagggtttt caatccctcc
    50161 acaacaaaag aactcaggtt atacggactt aatgatgatg atatttttga tattgaggat
    50221 aatgtaacat caagaataaa aatccggatc ataggaggca aagggtatga cacattcgac
    50281 ataaagggca atgttgaaac acttctctat gacctaaaag atggtgggaa ccatatcaaa
    50341 aacaatagcc gggctaaaaa tcgtttttca acagaagccc cggttaatga taaagatatt
    50401 ttcgggttta attataacac taccaggttt ccacagctgc atttcggcca gaatacagat
    50461 gatgggtttt ggataggagg agctttttca aaaaggactt tcggattcag aaaccttccc
    50521 tatgccagtg atcagcggct ggcagcattg tattcagtta gtcgcaaggc ctggcagttt
    50581 aattacgttg gagagtttaa tcatatcacc cggaaaaccg acctggtgat caaggccaat
    50641 ttctccaccc ctgtgctgag gaatttcttc ggttttggca ataacactga gatcgattta
    50701 tcaaagccaa aggattttta tcaaagcagg tataaattgt ttgaaacaga agcactttta
    50761 agaaaacgat tttttgagaa attgcatatc atggctggtc ctgcattcta tagttacagg
    50821 aacagcattg aggataatcg taataatttc tttggtaaac cagttcagaa tggacttgat
    50881 tctgccggca tatacagtaa aaaagattac ctgggaggga aattagtatt actgctagac
    50941 aaccgtaatg atgaactttt ccccacccgt ggtattcact ggcaaaatga atttgtttca
    51001 atcgctggtc ttaaaaaagg gaactataat tatactgcat ttacatcaga catgtccgtt
    51061 tatgcgagtt ttactgatcc cgcaaaattt gttgctgtat taaaatttgg tggcgggcat
    51121 attttcagca agaattatga atacttccag gcattaacgt taggagcaaa caacaacctt
    51181 cctggcttca ggaaaaacag gtatgccggc acgtctactt tgtacagtgg tctcgaatta
    51241 agggtaaaat tatttggcat gcagtctaac atgctgccgg gtcaattagg ccttacaggt
    51301 ttttacgatg caggcagggt ttggcttccg ggcgaacgtt caaaaagatg gcatggagct
    51361 tatggaggcg gcgtttactt tgtaccattc aacctgttta tcatctccgc tactgcagga
    51421 ttttcggaaa aggaaagaat gttcaacttt actgtgggta aacgaattaa tgccaggtat
    51481 taacaggccg ggtaattgat tatttcttac tatgaaatca atcaaagtaa taccaagtcg
    51541 tgacaggatt gttgctgtct ctgttatttc ttcagttata atatttactt taactttttc
    51601 atctgttttt ttcaccaatc agacctggat cgatgaaaaa gcatttcaga taatatcccc
    51661 cttcatcagc gaggaaagaa ccagcctgat gaaatttatt tcctccctcg gaagacacag
    51721 ctttcttatt ccggcaaacc ttgtactcat tattctgttt gcagtattgc acaaaaaatg
    51781 gatggctgta agagtggcat ttgttgcact gaccagcctt ggccttatgt cattgctcaa
    51841 aaatttactt cagcgtcaac gtccccccga accgcttgtg gaaggcatta caaatttcag
    51901 cttcccaagc ggccatgcgt ttatgggcat cacattttat ggactattaa tatggatgat
    51961 agctatccat gtaaaaaata aatggcaaaa aggtttggca attacttttc tgtcgctatt
    52021 aattattact atcgggctca gccgggttta tctgcgggta cattatacca cagatgtttt
    52081 agccgggttt agtattggct ttctttggct tatagcctgc ctgaatatta ttgaaaaagc
    52141 agaatcaggg tttgtaaaca aatcgtaata tagtagcctt acttatcact ccctcatttg
    52201 aaatatacat atcgccattg gacttaaagg tcaacccttc cggctgcgga aaaagtttct
    52261 ttccaagttc gaatactgct tctacttttc catccagatc aactattgcc agttgattac
    52321 ttgcagagga aagaataaat aatttttgta acaccgggtg gatagcggca gcagaaggtt
    52381 gaaattttga tgttttatga ggtgataatc gttccacttc tttagcatca atagtaaaca
    52441 ccggtttatt gtcaaaacca gttgagtcaa aataataagc aaaggcactt acgcttgatt
    52501 tattatccaa cgcacaattc ttacaaataa ggacaagtgc attacgggtt gggtcatagt
    52561 acatggcttc aaaatcattg accccggtta tcccgatttt acctaattct tcaccataaa
    52621 ttttaccggt actatcctta ctgactttcg ttatcatacc atcactccgg agcacatagg
    52681 gaatgctgtt gatcaacacc acatcttcat aatctccttt cttaccaaaa gtatattcag
    52741 ctttgatcac ctttgtttct ttatccaggt aaaataactt gccggcttca tcataatgag
    52801 ccacaaattc atcattctta ttatcccatg ccaaaccgga tatttctttc agtttaatat
    52861 caagcttatg tgtatacact tcaccgaaat tataatgggg tggagacttg atcatacggg
    52921 gcttcctgtt ctgacaacct gtactgctaa taaggagagc taataaccct attgcagtac
    52981 aggaatgtgg caagaaattt aaaaacttca ttcttctaat tttcattaaa tttatcaaaa
    53041 gttatctgta taacacgatg gaaaatcagt ccactctttt aaaagatgca caagatgctg
    53101 caaccaacct gatctcatcc aaagttagca agaatgtgag gtttcataca cttcagcata
    53161 cccgggaggt tgtagccgca tgtgaaaaga ttgctgaaac cttaccccta tctgatgatg
    53221 ataagatagc tttgaatatt gcagcctggt ttcatgacac cggctacagt gccggacagg
    53281 caaaagatca tgaaatgata agtgttcagc atgcagttga ttttttaaac gcacatgcca
    53341 cgagtgagga gatgatcaaa aaagtgacag gttgtatcat tgctacaaga atgccgcaaa
    53401 atccgaccaa taacatcgaa caaataattt gtgatgcaga tctttttcac ctgggctcag
    53461 ataattttaa agaaaagagc aagctgctgc gggatgaatt aaaggaattt ggcggagaag
    53521 aactttcaaa aaaagaatgg cggcaaaata atatcgcctt cctggaagcg cataagtatt
    53581 ttaccgcttt tggccaaacc aacctgcaac cggtaaagga aaaaaacctg gttgaattag
    53641 tagaaaaaaa accagaagaa atggccaaac ctgaaaagaa aaataaggat aagaaaaata
    53701 aaattaaaga acctgagctg gcagaacaag ctgcagcgga ggcgaaagca aaaaaattaa
    53761 aagaaaatca aacggaaagg gggatctcca ctgtattccg tatcatggca cagaaccaat
    53821 ctaacctgag ccaaatggcc gattctaaag ccaatattct catttcggtt aactcaatca
    53881 tacttaccat tgttatttct actcttttta caaaacttga gaataacagc aatcttcaga
    53941 taccagtcat actcctggtg gtggtttgtg tcagcgccat agttttttca atacttgcca
    54001 caaggccaaa cattacacat ggcacattta cccaggagga tattcataac aaaagaacca
    54061 atctcctttt cttcggcaat ttttttaaaa tgagtctgcc cgattacgat tgggcaatgc
    54121 aggaaatgct gaatgacaga aattacctgt atagcagtat tgtaaaagac aattatttcc
    54181 tgggagtggt acttgccaaa aaatacaagc tccttcgcat tgcctataat atttttatgt
    54241 tcgggctaat actggcaatg atcgcttttg ccattgctat tgcaatccca caggcaggtg
    54301 aaatttatac accgggaggt taaggtataa cgggtatact tacttctcta ttataaatag
    54361 caggaaacca catccgtcat tgctgattct ttactcctgt gatatgcagc tattgctcaa
    54421 aaaaaatcca ccggcaatag ccagtggatt tggaattcgt attcaaaaag atcaattagc
    54481 tcttcccagc atttcctcca gcttcatcac cagctctgtc ttggtaatag gcactcccat
    54541 tattttatta taccctttgg catccaccat ataaatatca cgtatgatct ttattagttg
    54601 gccattgttg atttcaggga tcagaatcgt atcgaaattt ttaataagat cacccaggtt
    54661 ttttgggaaa gggcgaatat gacggagatg tgcatggctt actgcatacc cttttgcctg
    54721 tatttcagag acggcagatt taatagcgcc gtaagtggaa ccccagccaa tcactaatat
    54781 ctttcctttg tcaggaccgc tgtctaattt ttgttctggt atataatcag caatcttatc
    54841 tactttctcc tggcggatct taaccatcaa ctggtgattc tccggttcat aattcacatt
    54901 accggtaatg ttttgttttt ctaacccacc aatacggtgt tccaacccta ttgtaccggg
    54961 aacggcccag ggccttgcca gcttttcatc ccgcaggtaa ggctgaaatt tttcttcccc
    55021 atgccccagt tcggttttaa attttacagt gattggcggc aggtcggctg actgcggata
    55081 cttccatggt tcagcgccat tggcaatata gccatcgctt aagaatataa ccggtgtcat
    55141 atgctgcaca gagatcctta ctgcttcata cacggcgtca aaacaatctg tcggtgtgga
    55201 ggcagaaatg attggcatgg ggcattcgcc atttcttcca taatatgcct gcatcaggtc
    55261 actttgttct gtcttggtgg gcaagccggt agacggacca ccccgctgaa tatttacaat
    55321 tatcaaagga atttccagca tcacggccaa tcccatcgcc tcccctttta gtgcaatacc
    55381 cggaccgctg gaggtggtta cacccaaagc tccgccataa gcagcaccaa tagcagaagt
    55441 aatagctgct atttcatctt ctgcctggaa tgtacgaaca ccaaaattct tatacttgct
    55501 caggtcatgc agaatatctg aggccggggt aattggatat gaaccaagga ataacggtaa
    55561 tccacttttt tgtgaagcag cgatcaatcc ataagcaaca gcctggtttc ccatgatgga
    55621 ccggtaggac ccaggttcca ttttagcttt ttcaacagaa tacgtggtgg taaaagtttc
    55681 ggtggtatca ccataattat atccggcctg caatacttta atattacttt ccagtatatc
    55741 gggcttctta ccgaatttct ctttaaggaa actgatggta ttttccatat cccggttgta
    55801 catccagtac aggaagccaa gtacaaacat attcttagcc ctgtcctttt ccttcattcc
    55861 cattgtaata tccttcaatg cctcacgggt catttttgtg acatccattt ttatcacttc
    55921 ataattaccc aggctgccat tttcaagcgg attctcgcct tcggggtaat tggccaggcg
    55981 aagatttttg gcatcaaaac catctgtatt tgcaatgatc ttcccgccca tttttaaggc
    56041 agtcaggttt accttcagtg cggcagcatt catagctacc agtacatcac aggcatcgcc
    56101 gggagtaaac accctgtcgg aagaaaatct tagctggtat ccgcttacgc cgggcagcgt
    56161 accctgcggg gccctgatct cagccggaaa atccgggaaa gtagccaggt caattccaag
    56221 taacgctgta ttattagtaa attgactgcc ggtcagttgc ataccatcac cactatcccc
    56281 tgcaaatttg attactacat catttaatag ttcctttttt cttgccatta ttgataagtt
    56341 attctgcgaa tttaaaacaa gttgtgcttg gttgatgcaa tgatttttat caggtaaaaa
    56401 acctctgcag atcaggggtt atacaaaagg gtttagatcc atttttttct accgcccggc
    56461 ataagaaaaa gtcatgcctg cagggtgccg tgttggaaac cgggatcaat tgtggcggca
    56521 gaatcaaagt cccctgctta gaaagtcccg ctaaatagcg aacaccctga aaatacaggt
    56581 gctaaggcgg accggcattg aactaagcag ccaggtattc cgatatcctg caacccttta
    56641 ttatacccgg gatgaaatta gttgctaata acttcctcaa acattgagtt ttctttacat
    56701 ttataaaaag tagctggtac aaatttgcat tgataatcat tacttttttg tgacagcagt
    56761 tgcgcctatt tactagttgg ctgctatata acaaccctca aattgtcaag tatctttaaa
    56821 caatattatc taaagaagtt aatcatatgg ggtggaattg gcctgattgc tttgtttatt
    56881 aggtattcaa gcaaaccaga aacttcgact ttgattggct tcgtaattat tttagctttc
    56941 cttagcgtcg gactaataaa cggtttcatc gattatcgct tctatgaaaa atcggctcct
    57001 agacttgtat caaatctcct agaacagaaa cctttattgg aatttaaggc tatcggcttt
    57061 tcaaaagagg atgatgataa aatcggcgga tacataaata attataaaat atttcttgct
    57121 ccgttgataa atgttgaagg tgaaaaatgg cttacagttt tgatcccatt gcaagtaaga
    57181 gaagggctcg ataactattt taacaagttt gacgattttt tcaaattcca attatctgac
    57241 aaagttcttt ttgcggaggc catcttgaaa agctatgata agacatacga ctatcagaag
    57301 atatttactt tactagaaaa aacaacgcta agtctacagg acaagaagat attgccaatc
    57361 aacatcgttg acgaatgatg tacagcgcac aacatgaaca ttgtaaaacc aaccactttt
    57421 caatctttta acgctcttac taagaataat tgggttaaaa caaactattg tagcctacaa
    57481 aaatttcctg ccgggggccg gtcccactac caggcccaat cgcttcactg attttacttt
    57541 aatctttctt taaccatcaa atacttgcag ccatccggct tcgggtataa tttcatcccg
    57601 gaattaaaca accacacact actatgaacc caagctcttc cgctgacgat ggcgaccctg
    57661 ccaaacagat ctttttacga cctgctcttt ctcttttttt tgtgctggca ctgctcacct
    57721 tctcctgtaa caataaggac actaaaaaag agacccctgc gctgcccata attgacagca
    57781 ccctggtcag gcagcaacaa caaaaagcag ctgactccat tgctaaagcc gctgcactta
    57841 aaaaaaagat ctatatcact tttgatgatg gacccaataa aggcacccgc aatgtactgg
    57901 ctgccgtgaa agaagaaaat ataccagcca gcttcttcat cgtagg
//
LOCUS       DAORTI010000009        51912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_365_length_51912_cov_8.452629, whole genome shotgun sequence.
ACCESSION   DAORTI010000009 DAORTI010000000
VERSION     DAORTI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 51912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51912
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_51912_cov_8.452629"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLO70_02520"
     CDS             complement(<1..785)
                     /locus_tag="PLO70_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ73354.1"
                     /translation="MSFQNKTAIITGATRGIGKAIALRLAKEGANIVIAAKSVEENAK
                     LGGTIFSAAAEIEAAGGKALAVQCDIRFDDQVQNVIDKAIEKFGGIDILVNNASAISL
                     TTTEQTEPKRFDLMHAINVRGTFFVTKACIPHLKKAANPHILTLSPPINMDPKWFEKH
                     VAYTITKYNMSMMTIGWAKELAKDRIAANALWPRTTIDTAAVRNLLGGTMLANMSRTA
                     DILADAAYYILRQPSGECTGNLFVDEQVLAKEGITDLEKYSVVP"
     gene            complement(886..1875)
                     /locus_tag="PLO70_02525"
     CDS             complement(886..1875)
                     /locus_tag="PLO70_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQZ73355.1"
                     /translation="MEYRYMGKTGLQLSVLSFGSWVSFHKQIDDSMADELMGIAYDHG
                     VNFFDNAEVYAFGESEKMMGRVLKKKNWDRTSYTVSSKAFWGWRGTANKPNQTGLSRK
                     HLVEACHEALQRLQMEYLDLFFCHRPDKGTPIVETVWTMHNLIQQGKILYWGTSEWSG
                     VEIMEAHRVANQYGLIGPSMEQPQYNLLERNKMENEFLMVFKTVGMGTTIWSPLASGL
                     LSGKYNDGIPEGSRFGLTGFDWLKDRWMKDDMLNKVKGLAALAKELGISMAELSIAWC
                     IKNPNVTTAILGATKKEQLLENLKAAKAVEKLDATVMQQIEDIVQTKPVLPEH"
     gene            complement(1948..2523)
                     /gene="lptC"
                     /locus_tag="PLO70_02530"
     CDS             complement(1948..2523)
                     /gene="lptC"
                     /locus_tag="PLO70_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQZ73356.1"
                     /translation="MLLFSVHKKIIQTAFFITGCFFMAGCENDDKVLQDWTKKVVMQE
                     EAIQVESYLSQDGKLKAKLKAPLMIRVAGDTISVEFPNKLHCDFYDDSTQLETWLDSK
                     YGIYYENLNKVYLRDSVVVITVKGDTLKSPDLWWDQSTKMFYTDKYATYHGVGKNIYG
                     GKGMEATQDLKRIMFKETTGNLKVSENGFPQ"
     gene            complement(2561..3979)
                     /locus_tag="PLO70_02535"
     CDS             complement(2561..3979)
                     /locus_tag="PLO70_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73357.1"
                     /translation="MQLVKLITPKIINTLHNKKWTFVILILAAVTPAFSQDNSPYSRY
                     GIGDLVPSTNIINRGMAGLSAGYIDHLAINFNNPASYSSFQSYKEKKSKKLSSGRAIL
                     DLGLNFENRTLREPGVTQKFTTSNALFSYVQVGVPLKQNWGLSFGLRPVSRISYKIFR
                     NERLIDPTTGLPIDSAFTGFEGDGGSYLASIGTGFSLFRRERKGLEEKLSVGMNGGYL
                     FGEKDYSTRRTLLNDTVSYYRTNYETRTNYGNLYFNAGVQYKLPLNTKMLLTIGAYGN
                     WAQKMNGRQDKLRETFIYDESLGNVRLDSVSDLRDIKGTVELPASYTFGFVLQKYAVP
                     NKEGGWLFGVDFSQQNWSQYRFYGQADSLQNKWEIKVGAQLSPVPKRNYFSNIAYRFG
                     FFMGPDYVKVGQKLPQIGGTFGLGLPVGISRQAPNQITFINMAFEYIKRGNDDNLLRE
                     NMFRFSLGFSLSDIWFVKRKYD"
     gene            complement(3951..4679)
                     /locus_tag="PLO70_02540"
     CDS             complement(3951..4679)
                     /locus_tag="PLO70_02540"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQZ73358.1"
                     /translation="MSVTLCFDFGNTRKKVAVFHGAEMKEAQVLPDDSIETIQSLINN
                     FKPGKSILSSVIDHNPLIEELLAATTKFHKLSHLTKVAFTTPVGKPETIGADRLALTA
                     AAVHYYPTYNNLVIGMGSCITYNFINKYHEFVGGAISPGLEMRLKALNYYTAKLPLIK
                     ADSNVPLIGYDTNTNILSGVVLGMAKEMDGFIEAYREKYRNINVLLTGGDIVYLASHL
                     KNKIFADPDLIFKGLYAISEVNNP"
     gene            complement(4709..5344)
                     /locus_tag="PLO70_02545"
     CDS             complement(4709..5344)
                     /locus_tag="PLO70_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQZ73359.1"
                     /translation="MTNFIPIFPLGIVVYPGESLHLHIFEPRYKQLISECHATKKTFG
                     IPAVIDNKMKEYGTLVEITELSKVHENGEMDIKTRGVKVFRILEIIKEVPDKLYHGAI
                     VTYPDNYKTGNPELMRKVMSSIRDLHRLLKVTKDFKKEDEKMNSYDVAHHIGLSLEEE
                     YELLCLADERQRQEYLKRHLIKAIPVLAGMEQLKEKIKLNGHFKNLGGFEW"
     gene            complement(5405..6169)
                     /locus_tag="PLO70_02550"
     CDS             complement(5405..6169)
                     /locus_tag="PLO70_02550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQZ73360.1"
                     /translation="MSQFSIVIICRNEAGAIAKTLSSLQGLTDDIIVYDNGSTDGTQD
                     LVRQFKVHLHEGSWEGFGKTKTKANQLAKYDWILSLDADEAADEELRQSLLQWNPGND
                     VIVYDILFKNFLGEKYLKYGEWGGDHHIRFFNKKFVQWDEAPVHEQLIIPANAVIKKL
                     KGYVLHQTMKDIHDYAAKMVKYAMLNGEKYYKQGKKASWFKLRLSPGFTFFNYYILKL
                     GFLDGHAGYICARMTAYYTFLKYARLKELNKGGENR"
     gene            complement(6195..7418)
                     /locus_tag="PLO70_02555"
     CDS             complement(6195..7418)
                     /locus_tag="PLO70_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73361.1"
                     /translation="MLNKLQNERRNTIIFILFIVMTGALFISRALLSASMIVFILVSF
                     LHSNSKKQVYDFFSTPLLWGMSVLFLIPLISGLWSSDKKEWLDMIRIKLPLLFLPIAF
                     ASPFSFTKKQWEWLGYIFIALITGGTLWCMYHYLQDTAVIHEGYLRAKTFFTPLENDR
                     VRFSWLVSIAALLAGWLAFSKQKSERTTSLVLITVAGWLAVFLHILAVRTGLFSFYSM
                     LFMTAAWMIIKNRKKGYSVILLILLIAMPVTAYFTLPTFQNRIKYINYDYGYFSKANY
                     LPGGNDALRVISIKGGWNIMKENPVTGTGFGDVLTETRLWYASHYPGMREEDKLYPGS
                     EWMMYGAGSGIPGFVLFTVVMLIPFFVQVKDKILWWLLNATAAFSFLFDIGLEVQFGV
                     FTWSFIVLWWWKWLK"
     gene            7452..8348
                     /gene="meaB"
                     /locus_tag="PLO70_02560"
     CDS             7452..8348
                     /gene="meaB"
                     /locus_tag="PLO70_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQZ73362.1"
                     /translation="MWQELLKQIQQGDTKALARSISLVENEKPGYETLLQSLPASTTK
                     IIGITGPPGAGKSTLVDALIGLLINTGKKIAVLCVDPSSPFNHGAVLGDRIRMSDWYT
                     HPNVYIRSLATRGSMGGLHPKIIEITDLLKAFQFDHIIIETVGVGQSEVEIAGLADVT
                     VVVVVPEAGDEVQTMKAGLMEIADVFVVNKADRPDADIFVRNLRSMLAPSFSKTYNEV
                     PVIKTVASQKEGISELSAIILDLLQKAQLNDKTFWLLAEKAYYLIEQKRMKDVSKAEL
                     KKEIEVCFKKGSFNLYKFIADK"
     gene            8381..10276
                     /locus_tag="PLO70_02565"
     CDS             8381..10276
                     /locus_tag="PLO70_02565"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ73363.1"
                     /translation="MKNRNHIVKGFIVLCVWLTFFLLPVSATAQTTNISGIVNTYHRV
                     VEIIPSKACIRVASVTGLTINTKVVLVQMKGATINTSNSAGFGTVTSLNDAGNYEIGT
                     ICSIIGDSVFLFQNLLNTYSPAAGKLQLVQFAEYYSANVIDTVKAAAWNNTTGTGAVI
                     AIYADQDITLNAPVYADASGFRGGNFLQSNGTCSDAIPASGYIYTASNTSPQNGAYKG
                     EGIADVTSGQSGGRGSPANGGGGGNNHNNSGGGGANLTTGGIGGGNSSNGVACTATLR
                     GEAGKSLSSNSGQKIYLGGGGGAGHSNNGLSNVSGGNGGGIVFIWANQLIGNNRIITA
                     SGGNGGNSLSDGAGGGGGGGTIIMHVSNYTGNVTVQANGGNGGNSDDGGNIGRCFGGG
                     GGGSGGAIYFTGALPAITTDITGGAAGVESGRDVSCDAIPQAGGAGSNGQVITNYTFS
                     RSTNPAGYCAFLLASGLNYFNAVRMGNLVLLNWKTASPAEIQYFEVEKRNAANSWNSI
                     SIVSGNDATREYSATDNNPQQGYNYYRLKITGRNNAVSYSEIKRVYIRQAGAEFEIYP
                     NPAISKITISRKNSLPGQLKITDITGRIIHQQTISSGSAEINLPTIAKGIYLVIIDTV
                     VKKLEIR"
     gene            complement(10282..10503)
                     /locus_tag="PLO70_02570"
     CDS             complement(10282..10503)
                     /locus_tag="PLO70_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73364.1"
                     /translation="MKKWIVVIGLFFSLLLFTGLDSQAQCSVCTKTAQQLGEKPAKGM
                     NSGILYLAFAPFAIVGLISYRWWKNNKNE"
     gene            complement(10560..12647)
                     /locus_tag="PLO70_02575"
     CDS             complement(10560..12647)
                     /locus_tag="PLO70_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73365.1"
                     /translation="MYSRILFVFLASMISSVTVAQCLSGDCKDGFGKFKYKNEDVYEG
                     YFKNGYCHGKGKMSYVNGSLYDGDWINGNWNGKAKYFWKDGRRYEGEVKDGYFEGYGI
                     FYAADSGRYDGYWKVGKYDGQGKYFYSNGRIYDGSWLKGVRSGKGKFTWPDGGSYDGN
                     WKDDKFEGYGKRIYKDSSSYEGEWMADKQHGKGKYVYNNGDIYTGDFKDGKRSGTGKY
                     ITKLDGQTYEGEYAEGQAEGKGKLVLGNKDRYEGDFKKGLAEGYGVGVYADGKKYEGQ
                     WKNGKWEGNGKCTWKDSSSYDGQWVNGKANGTGTKIYKGGDKYVGEWKDDKINGEGVY
                     TWKNGDSYTGSFRGDVMSGKGKKIYQDGTIYEGDWSNGVYAGTGVWSWPNKNKYEGKF
                     VAGNPSGNGKFYRNDKLFFEGSFDGTSTYSSAKNWILKDGTYARRTTFSDGSFFEIWF
                     PDNSVFFGSINEIGYYGSIAEIQPKEGFLLNPSGKIMVGKVENKTIKNPGYTYTIKMS
                     EAVPDYVKGRALFAYKKNAEALVAFNKAIAQNIKEDSVYLYRGIVYSNLKKADSALMD
                     MTQMLKKQPKNKDALTWRAKIRLTNKDTTAALADYDTYIKFYPKDSAGFWQRGVLHHT
                     RKDYVKAIADYSKVLEFAKGANDNVYYYRGICYELLEKKTEACADFEKAKKAGNKNAD
                     ARIKKLCSPPAGG"
     gene            complement(12829..14154)
                     /locus_tag="PLO70_02580"
     CDS             complement(12829..14154)
                     /locus_tag="PLO70_02580"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQZ73366.1"
                     /translation="MTMYFLNKIKCLSLFICILAISCGKGENTPAPVPVNSDPVIFSI
                     APLSGGVGTTVIIKGDNFSTNILSNIVKFNGVIATISVAKEDTLIVTVPAGASNGAIT
                     ITVNNRTVNGSSFTVLTNLTVSTLAGTGVQAVLNGNASAAQFNFPYDICLDAQGNIYV
                     SDYNQIRKITPAGIVNTLAGSGIRGFLDGPGNIAQFNPVMGIACDGQGNIYAADRDNN
                     KIRKITADGTVSTLAGGSTFGYQNGTGTDARFWEPISVAVDAQGNVYVADKSNFRIRK
                     ITPSGTVSTLAGKGTSGYSDGISSLAEFYNLQDIAVDNQGVVYVTDQNRIRKITSDGT
                     VTTFAGSGANNFLNAIGIMAEFANPNGICIDGAGNIFIGDVTNAVIRKISTTAVVTTY
                     AGSPGQQGFADGLFNTAKFYYPKGVAVDIQGNIYIADNANHRIRKVSFQ"
     gene            complement(14242..16053)
                     /gene="typA"
                     /locus_tag="PLO70_02585"
     CDS             complement(14242..16053)
                     /gene="typA"
                     /locus_tag="PLO70_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQZ73367.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKILHTTKVFRDNQETGDLIMDSN
                     DLERERGITIFSKNAAVVYKGIKINVIDTPGHADFGGEVERVLKMADGVILLVDAFEG
                     PMPQTRFVLQKALHLNLHPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLHFPT
                     YYGSGKNGWFNDSLEQIDNINPLLDGILKHVPPPKVNEGPVQMQITSLDYSSFLGRIA
                     VGKVNRGVLKEGQNVVLMQADGTIKKTKVRELYVFEGMGKKKVTEVVAGDLCAVVGIE
                     DFNIGDTIADAENPEALPVISVDEPTMNMLFSVNNSPFFGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDSEDGDSLMVYGRGILHLGILIETMRREGYELTVGQPQVIVKEINGKK
                     CEPYENLVVDVPQEFASKVIDLVSRRKGEMLIMETKGEMQHLEFEIPSRGLIGLRTQM
                     LTATTGEAVMAHRFTEYKPWKGPIPGRSNGVLLSKNTDKTTGYSIDKLQDRGTFFVDP
                     AEEVYAGQIIAENIKPGDLVVNATEPKKLTNHRASGSDDATRIAPKTQMTLEECMEYI
                     QQDECIEVTPNFIRMRKVILNEDERKKVQKKMETEAV"
     gene            complement(16094..16468)
                     /locus_tag="PLO70_02590"
     CDS             complement(16094..16468)
                     /locus_tag="PLO70_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ73368.1"
                     /translation="MAYQSFEELEVWKKSRDFKNEIAQLVRTFPTNERLRLSDQLIRS
                     SRSINAQIAEGHGRNTWPDKIRFCVIARGSLSETLNHLIDALDEKLISEEQLKYYRIK
                     ITEVEKLLNGYINYLEKRAKEK"
     gene            16655..17647
                     /locus_tag="PLO70_02595"
     CDS             16655..17647
                     /locus_tag="PLO70_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ73369.1"
                     /translation="MKFLKWLGIILLVLIVIYFLGPRPATPKYTNDLPAIPAESSMLE
                     QYIRNNEAKHKLKPDNEARILWLNDSIKAKTEYAVVYLHGFSASQEEGDPVHYEFAQK
                     FGCNLYLSRLDAHGIDTTEPLGNFTAEGLWNSAKEAYAIGKQLGKKVILFSTSTGGTL
                     SLKLAAEFPDIAGLILLSPNIAINDPNAWLLNNHWGLQIAHLVTGKYRTVEDTTSIYA
                     QYWNNRYVTKSLVQLEELLESTMKASTFKKVTQPTLLLYYFKDEENQDPVVKVSAMKR
                     MFNQLGTPDSLKRQVAIPDAGNHVLGSYIKSKDIEKVQEECEKFAMEVLKIKDR"
     gene            17739..18224
                     /locus_tag="PLO70_02600"
     CDS             17739..18224
                     /locus_tag="PLO70_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73370.1"
                     /translation="MKKQLLIIFVGLFVCSACDKDKTDKPYVPIIRCPDMTRNMDTIN
                     NYIRGNWEWVEEYRVTRTFQGWVTPNTPGENHLSLKLYGDTLQFFTNNRPDSFYNFRI
                     RLLSDLTNYPDDSLPIIYYTSFYTGMGGGVIPIKICKNQLLMQHQYVSSIIGERLWIR
                     K"
     gene            18318..20126
                     /locus_tag="PLO70_02605"
     CDS             18318..20126
                     /locus_tag="PLO70_02605"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQZ73371.1"
                     /translation="MNKFFVFTVLLIASKSFSQGFTNRCGTIADMNLIQTNTPNVYQS
                     YLDIEAFTANYITNQTQTNSRLINGAGLIVIPVVIHVLHNGEAEGTGLNISLATIQSQ
                     LDVLNEDFRRLNPDAINTPSAFQGVASDFGIEFKLACIDPNGNVTNGVVRKQVSASQY
                     NISVRTDGSYDDDAIGIKKSSTGDAAWPTDHYLNIWVCKMNNGTAGYGTFPFHYSTYP
                     QFDGIVIDKGAFGRNGGTTSGFDKGRTATHEIGHWLNLSHLWGNTISPQFNFDCFSDD
                     LVGDTPQQKTYNSGCPSFPEILRKCNSVDPSSMFMNYMDYTNDQCYNLFTNGQRLRAR
                     ALFATGGPRATFLINYFQIQTPTKVSCNGSVIKLFNPTCATPTWSIVSGNATIVSGQN
                     TNSLTVSTPSPGSFVLRATGGNYIEDKTIDVVPFTSSDYTLVGNNGSMYWCPNQTTSF
                     SVSGQGATNYNWSLPTAWTMINNGGSYVVVRAPSSTYPPTGTLSVSVTEPCGTIIAIN
                     KFLAYSSSACSPSIPFTLYPSPASTYVNIEVTNSQTNIAAVQITDMYGNILINNQYSG
                     NYTGVQIGVYALQNGPKIAKIYSGTQWYSIQFTVTH"
     gene            20151..20306
                     /locus_tag="PLO70_02610"
     CDS             20151..20306
                     /locus_tag="PLO70_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73372.1"
                     /translation="MKQNYFPSFYQAAIPDAGNHVLGSYIKSKDIEKVQEECEKFVTE
                     VLKINQQ"
     gene            20332..21699
                     /locus_tag="PLO70_02615"
     CDS             20332..21699
                     /locus_tag="PLO70_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73373.1"
                     /translation="MTSKWSRRDILRLAGLSATALPLSSWATNENGERIILPDNPQHK
                     KLKKPVTAITLGAGSRGNVYGNYAVQYPDQLDIIGVAEPIAIRNERYTQKHNIAAENR
                     FQTWEDVFKKPKFADAIIISTPDNLHYGPCIEALKMGYDILLEKPISPSEKECRDILQ
                     LAKKTGRIVAVCHVLRYAPYFIKLRELMNSGAIGEVVSIQHLEPIGHVHMSHSYVRGN
                     WHNSKETTPIILAKSCHDLDILKWMVNQPSKKIQAFGDLKWFKKENAPEGSTERCTDG
                     CKIEAECPYSALRIYQRNRSWLQVMDMPDDKEKHAAHILEKLKTTNYGRCVYQMDNDQ
                     PDHYICNIQFENNITASFSMEALTSYEGRRTRVMGSMGDIVGDMNSFVLTDFRTGKKT
                     EWKQSTDGHGGGDWRLVADWIQAIDQQNPSLLTSTIDASIESHVMGFAAEKSRKENKV
                     VTIEM"
     gene            21837..22901
                     /locus_tag="PLO70_02620"
     CDS             21837..22901
                     /locus_tag="PLO70_02620"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQZ73374.1"
                     /translation="MHKSKIAGIGMYVPANVVTNNDLLKYMDTSDEWIQERTGIKERH
                     YAHRTEETTTTMGVEAAKIAIERAGITPQDIDFIVFATLSPDYYFPGCGVLLQRALKM
                     KEIGALDVRNQCSGFLYALSVADQFIKTGMYKNILVVGAEKHSFGLDLSTRGRNVSVI
                     FGDGAGAVVLQPTDPSSGAAQNQGILSTHLHSDGESAEILAMYNPGTHANHWKEEKYA
                     DFDEAEIAQMFFSHAMIDNGQIFPNMDGPAVFKKAIVKFPEVILESLKANSLQTADLQ
                     LLIPHQANLRISQFVQQKLQLRDDQVYNNIQHYGNTTAASVPIALCEAWEKGLVKEGD
                     LICLAAFGSGFTWASALLRW"
     gene            22975..23397
                     /locus_tag="PLO70_02625"
     CDS             22975..23397
                     /locus_tag="PLO70_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73375.1"
                     /translation="MKKSILLFSLIVMSVTMHAQQTWTISHQKKTVLKNISEDPDNNV
                     VTISKALLNTTGCFVITFKAYDTAFTRTVMAYDTVRNGLKNWDDVKKTISIPNAELKS
                     VMAGKNKINLYYSEIPKDPEKAAVIRVRLIHICTIILK"
     gene            23450..24358
                     /locus_tag="PLO70_02630"
     CDS             23450..24358
                     /locus_tag="PLO70_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HQZ73376.1"
                     /translation="MSRNIIYIINPVSGTRTKKDLQQLIEKKTTEAGIKFQVFPSVAS
                     GDYAFLHPIIKEQQVTDIVIAGGDGTVSQAISSLMNCGVNFGIIPCGSGNGLAYAAKI
                     PKQPAKALDVIFNGNPTAIDGFTINKHFACMLAGLGFDAKVAHDFAKQPKRGLSTYVK
                     QVIKNFWAAKTYLFELTIEEKKFIIDAFFISIANSNQFGNHFTIAPKASLADGLLDIV
                     IVTHQNKLSLLFQTFKQVLGKNKLQQSAVFENKKGVIYFQTDKIAIANQSEAPLHIDG
                     DPAETPTKVQVEIKKKCFRLIQPSDH"
     gene            complement(24336..26606)
                     /locus_tag="PLO70_02635"
     CDS             complement(24336..26606)
                     /locus_tag="PLO70_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQZ73377.1"
                     /translation="MKAWLKGFYYSFPIQLLFLHFRKYQVLLLFWVILFSTVNGTFMK
                     TFGADSLFLAPEYLGNVNSISYAIVGASVGMFIMSWNITSFILFSRHFRFLAAATNPF
                     LKFCINNSIIPGLFLVFYFFKAYHFVHFKELVPRTEILFFFGGFTSGFIFILAASLIY
                     FFRADRSILQRMMPVIAEPKNYITHLGIIKEVYHDKPLIKVNSYLETPFRLKPARDVS
                     HYTEEFVESMFRGHHFAAILSVFAAFLFLILIGFFLDYPFFQLPAAGSITIFFSILIG
                     VAGAITYFLQSWSIPYLVGLVILLNVFYKLDLIDPRNKAYGLDYWNKNERPAYTKEGL
                     LNLCSKENVEADRQNMITVLNAWKKKQESDKPLLVLINTSGGGHRSATFTMGVLQHLD
                     SITNGEIMKKTFLINGASGGMIGAAYFRELYLRKLQGDNIRLQNKQYIDDIAGDLLNP
                     LFTSFFARDLIAPAQKFSVGPYRYVKDRGYAFEQKLNENTRGFLNKQLGDYVDVEKSA
                     KIPLMFFNSVITRDSRKMIISTQPVRFMMQGQSDSSKIPVADPDAVDFVSFFNKQDPY
                     NIRMLSALRMNATFPIVLPNVWLPSNPVIDVMDAGLRDNYGQETTLRFLSSFDDWIKE
                     NTSGVVVIQIRDRQAGGWENPYLSDDITDHATKPFLLLQHNWFKMMEYSQNDLLDYFT
                     TTTATPVYKVAFQYASSNQENKAALNFHLTRREEKDIISSINSDINRQGFNKLLQLFS
                     QRDFRESVSASDQKVE"
     gene            complement(26681..27751)
                     /locus_tag="PLO70_02640"
     CDS             complement(26681..27751)
                     /locus_tag="PLO70_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQZ73378.1"
                     /translation="MTNELTDRSSKPVATWLLIGVGMIIIQILLGGITRLTGSGLSIT
                     EWNVVTGTLPPMSEQQWFAEFEKYRQTPQFNLLNFDFTLKDFKFIFFWEWFHRFWARL
                     IGVVFAVPFIIFLIQRRFKPAMVKPMIILFLLGALQGAIGWIMVASGLTGDAIYVKPT
                     KLALHFIFALGLLSYTFWFAMELLVPDKKFVISRSIRNLTLLIMAVLVVQLVYGAFMA
                     GHKAATAAPTWPDINGYLFPPGMMGNEHGLLNLVDNKITIHFIHRGLAYILLGLVLFL
                     TLKLYKTPGSSLYNKTRWWPLLLVFIQVVLGIFSVLTSIKIIPNQWGVFEWMAQLHQF
                     VAMLLLLSLVWILFIVRNKAAY"
     gene            28163..29095
                     /locus_tag="PLO70_02645"
     CDS             28163..29095
                     /locus_tag="PLO70_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73379.1"
                     /translation="MEDDGTLQLKNQFEFSPKFQIKQFIVTETDSYLVYGAEMYFLGK
                     KTSFIKEWQGKTGFSEFSFSAESSSSIKNIFRDRNFTSVIACQFSQFSMRPAFYTVNS
                     KEKTLFVKTPDSVHLNYDFMSDFSEGKYYIAGNSRINTDSTDKSPFYSRLLLFDKNGS
                     LRKEEIYQLDDSLHHFDVRSLSVQNEEVAITGLLYYRDYKNLERRWIVQAVKKGKLLW
                     SDTLPEGYMPQSVHHTGNSIIVVSLFTNKPGKGYNSYHFLRYNKHGELLENFSLDNIK
                     PEWKNIVVVHVSEKELVFAGKNIFNSLVIEKISF"
     gene            29099..29728
                     /locus_tag="PLO70_02650"
     CDS             29099..29728
                     /locus_tag="PLO70_02650"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQZ73380.1"
                     /translation="MLAQYFQTALTEAGLDEAGRGCFAGPVFAAAVILPKDFYHPLLN
                     DSKQLSEKLRNQLRPVIEREAVSFAVASIDNEEIDQINILKASFKAMHLALDNLKKRP
                     QLLLIDGNRFIAYKKIPHQCFVKGDSRFASIAAASILAKTYRDEWMQQLHREFPHYSW
                     DRNKGYGTATHRKAIEEFGLCKYHRKSFRLFPVQTELFETGQNDTYKSS"
     gene            complement(29725..30333)
                     /locus_tag="PLO70_02655"
     CDS             complement(29725..30333)
                     /locus_tag="PLO70_02655"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQZ73381.1"
                     /translation="MLLQIHPKNPPPRLIKQIVESLQQGGVIIYPTDTVYGLGCDILQ
                     QKAVERICRIKKVEPRKAQLSFVCSDLSHLSEYAKPISNPVYRLLKDHLPGPYTFILP
                     ASKMVPKILQSKKDTIGLRIPDNNIAMAIINELGRPILSASLPGEMVEDYTDPEVMYE
                     NFRNEVDYVVDGGIGGTIPSTIVDFTGSEPILIRQGLGEWEN"
     gene            30439..31317
                     /locus_tag="PLO70_02660"
     CDS             30439..31317
                     /locus_tag="PLO70_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HQZ73382.1"
                     /translation="MQLKPVAVFDSIDPEVFKKEYYEPCLPVVIKNLAKDWPAYSKWN
                     WDYFKQLVGQKKVPLYNNVKSDAYTPINTADDYKTFGEYIDMISSGPAAWRIFLFNIF
                     DHAPQLINDFTWPEHLMKGFVKKYPMLFTGGASSITHMHFDIDMSHILHTQFCGRKRV
                     LMFPYQEQYKLYRKPWEVLSLADFSNYYIDGKVDYEKFPALKLAEGHDFILEPGDTLF
                     MPAGYWHHMEYLDSGFAMSLRALQPSVTGKLRGAWNLFGMRSIDTVMKKTVPKWWYER
                     KEKRVFENAARVLASN"
     gene            complement(31350..32072)
                     /locus_tag="PLO70_02665"
     CDS             complement(31350..32072)
                     /locus_tag="PLO70_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ73383.1"
                     /translation="MKQLLISIFFILSVSVANSQFSFTYNGREYRVLKTSVFTDSTGK
                     QYNYNEAMLVMMGGEYEILPANPKDASKGFLLSGISREEQLKRAMAAPKPAETTAFKT
                     GKKFDPFSAYDMKGNLVDTRQLKGKVLVINFWFTTCPPCKQERPYLNKIVDDYKSDSN
                     IIFIAIALDQKEQLEPYLKNNEFKYNVIPAGKKIADSYKITGYPTQVIVDKEGKVAFH
                     TVSYYYVTDYWMRKTIDELKKS"
     gene            complement(32159..33187)
                     /locus_tag="PLO70_02670"
     CDS             complement(32159..33187)
                     /locus_tag="PLO70_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQZ73384.1"
                     /translation="MSEVTIAPALTAKDFATDQEVRWCPGCGDYSILAQVQKVMPTLG
                     IPKENIVFISGIGCSSRFPYYMNTYGMHSIHGRAAAIASGLKASRPELSVWIVTGDGD
                     SLSIGGNHTIHILRRNFDVNILLFNNQIYGLTKGQYSPTSEEHKITKSTPMGSIDHPF
                     NPAALALGADATFVARTMDRDPKHLQAMLLRANGHKGASFLEIYQNCNIFNDGAFEIF
                     TEKGSKPAETLFLEQGKPLVFGAANDKGIKLDGFRPVVVNLNEGHSVDELWVHDENDI
                     YKAQILTRIFDDPHNEGHLPRPFGVFYKTERTCYEDAMAAQLEEAKSRKPADLDKLLK
                     GNEVWTIS"
     gene            complement(33268..33618)
                     /locus_tag="PLO70_02675"
     CDS             complement(33268..33618)
                     /locus_tag="PLO70_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQZ73385.1"
                     /translation="MSNLVEDFNAYRTKMNEIILSKDNLVIKRLWNLDTNTYAEGALD
                     VKTKELLGLVASMVLRCDDCIKYHLGKSHELRVTTEEMYEVFAVANIVGGTIVIPHTR
                     RAAEYWEELINDKS"
     gene            33724..35934
                     /locus_tag="PLO70_02680"
     CDS             33724..35934
                     /locus_tag="PLO70_02680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQZ73386.1"
                     /translation="MRKIILVLFILQSAIFSIAQTGKEPVKVTDLLKIKSISGVNLSK
                     DGGKAIFTVTSIEPDSDNKLEYKYITQVWMVNTDGASSPKQLTTHKENSSQAVLSPDG
                     KQIAFVRAVDNKQQVFLLSLEGGEASQLTKHKYGAGNPKWSPDGKQILFSASIPLKDL
                     LKDSTLNPDKELPKWPMEKPGIDNKLTLKATTSKADPDGTIEEIRAYLENNAIDKKAN
                     VINKLNFLDEMNINAEMSFNHYFLIDLRPDTKPVPVTRGFYRYNSADFSPDGKQLILA
                     GNMDSLQHPDRALENEIYMVNTDGTKFRKVVGEKDKNFNSPRISPSGKWLTFQYGNTS
                     FVSVPQLAIMPVNGSVKDKIDIPFDRSKGNITWSNDEKYIYFTAQSNGGQPIYRVDLS
                     SGWTIYPPLSTLKDKDKNIRVDQLTDYNDGIGSFDIAGNKLVFVKTEVANPFEIYVAD
                     ATAKNQKRISNFNTEWIANKKLSFPEKHSFKNEKGMEIEYWVMKPANYEPGKKYPLLL
                     EIHGGPSAMWGPGESSMWHEYQYFCSKGYGVVYCNPRGSGGYGLDFLRGNINDWGTGP
                     TSDVLASLDKTVAEGWADTSKLLVTGGSYAGYLVAWIIAHDQRFAAACSQRGVYDLAT
                     FFGEGNAWRLVPNYFGGYPWDAAAKATLERESPINHVQNIKTPYIIFHGDNDRRTGFV
                     QSEMLYKSLKVLGRPVEYVRHPAATHELTRSGNNRQRIDQMLRAYEFFERWIKK"
     gene            35987..36382
                     /locus_tag="PLO70_02685"
     CDS             35987..36382
                     /locus_tag="PLO70_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ73387.1"
                     /translation="MYSNKIKVITGLSIFVLLGVAAVQAPHQKEKNLKILPKDISDEK
                     LDSIMQTYNKALGVKCDFCHVKQKVFPGDFDYASDAEPMKENAREMMRMTIEINKSNF
                     YFDKNIRPEYLNTVTCKTCHRGEPFPPED"
     gene            complement(36386..37552)
                     /locus_tag="PLO70_02690"
     CDS             complement(36386..37552)
                     /locus_tag="PLO70_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQZ73388.1"
                     /translation="MIRFLRFVCVFWAFSTNAQTIQNNFIAGKTTGILPFLEYGLGID
                     RLGGAKMTYLDTGVTIKVVDSTIVNYKVQLSKDHFAYLPKQNFQRENDIKLLPYYLTN
                     SWRVSGDDRYDYVTVTLDEKLPYRSMQQINPSKIVVDIFGATGNTNWITQLSTAEEIK
                     NVYHEQLEDDVFRVIIELKNKQHWGYSIYYQEKRLVIRVKRQPAEPDLKNLKVAVDAG
                     HGGDNNGARGITTKILEKEYTLLFAKQLEKLLLDENADVFMTRDKDTTLNMPDRILTL
                     QKEDPHFLISIHLNSSARDSIKGTSTYYRHIGFRPLSQYILKDLLELGLKEFGNIGSF
                     NFALSGPTEYPNCLVEVAFLSNKEDEQLILDPEFHKAVAAQIIAGIKEWLKSCK"
     gene            37750..38532
                     /locus_tag="PLO70_02695"
     CDS             37750..38532
                     /locus_tag="PLO70_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73389.1"
                     /translation="MTWNSIMGFVSSFALFVPILLILILRLGLYRSFPFLLVYYCSVL
                     VYNFLTEGYIKASDDIIQVWGLTNNLLDTPLILFFLIYFSTSKSFTQKMKLLAFTFIL
                     FEAIVLTTIGFNIDAITIIMGPGLLIICGLCLYFFIRQAKLAVENRKATGKAVIITSL
                     LFGYGCYGIIYLMYYIFKAHFRADGTVNQQYVADTFLVFFIATTLSSIIMCIGLVIER
                     KRIQKLQELKVTRKELSAIYTDTKRAAPLRTAMLDFDRDQWN"
     gene            complement(38529..40322)
                     /locus_tag="PLO70_02700"
     CDS             complement(38529..40322)
                     /locus_tag="PLO70_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQZ73390.1"
                     /translation="MSTVTATKTVLKGAEWLVKESLPFESFTPENFNEEQFMIRDMCN
                     QFLEAEIYPILDRIDSLEPGLMKSLVTKAGEQGLLATSFPEQYGGLGKDFITSTIINE
                     YLGAGHSFSVAIAAHTGIGTLPILYFGTDAQKEKYIPKLITGEWAGAYGLTEPNSGSD
                     ALGAKTTAKLSDDGKYYILNGQKCWITNGGFAQVYTVFAKVDGDKFTGFIVDRGTEGF
                     TQGPEENKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHKIAFNILNIGRLKLCAAAL
                     GGARKSLSGTVEYAKTREQFKQPISNFGAIKHKLAEMAIKVFVAETALYRTAKWIDDK
                     EHQLLAEGKPFSEALLGGAEEYAIECAILKVYGSEVLDYVVDEGVQVHGGNGFSAEYN
                     ISRAYRDSRINRIYEGTNEINRLLILDMTLKRAMGGRLDLMGPAMAVQKELMSIPDFG
                     AEDDAPFAKERKLVANLKKAILMTSGAAVQKLMMKIENEQEVLMNIADMAIETFNAES
                     VLLRVMKMADQKGEAACQDYLDMMRTYLYDAADKVNKSGKDAVNSFADGDEQRMILMG
                     LKRFTKAEPFNSKEARRRIADKMITEGRYCF"
     gene            complement(40403..40843)
                     /locus_tag="PLO70_02705"
     CDS             complement(40403..40843)
                     /locus_tag="PLO70_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQZ73391.1"
                     /translation="MPNNQFKKGELYSFITGKASTAIARRLQKKFNAAGLNITIEQWS
                     VLYHLWKQEGISQQELCNATFRDKPSITRLVDNLEKLNLVKRVASESDRRINLIYLTK
                     QAQKLQEETMALADETLNEALITVPADKVDICKEVLQVVYDNLK"
     gene            40930..41736
                     /locus_tag="PLO70_02710"
     CDS             40930..41736
                     /locus_tag="PLO70_02710"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ73392.1"
                     /translation="MELKHLIYRNSTIGYYCFGSGLKQAVCFHGYGEDASIFHFLSNY
                     AGNQYTFYSIDLPFHGRTEWNEGLNFTRQDLVLIIEKITEKAAKELTLIGFSLGGRIS
                     LSLFEAIPEKIEKIVLLAPDGLKVNFWYWLATQTWLGNRLFALTMKKPGWFFGLLKMM
                     NNVKLVNASIFKFVNYYIGDAEVRRLLYSRWTSLRKIKPDLPVIKNLIRENKTTVRLI
                     YGKHDRIILSSTGEQFKKGIEEYCTLTVIHSGHQVLHEKHIKEILPALMQ"
     gene            41756..42871
                     /locus_tag="PLO70_02715"
     CDS             41756..42871
                     /locus_tag="PLO70_02715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ73393.1"
                     /translation="MIILYTTILLFLLYSLLIIYYWQAWKSIPDYSMSFTLQQTKISI
                     IIAARNEEENIGTLLAALQRQTYPAAFTEIIVVDDHSTDSTVAIARQFPRIKIIQLKE
                     DNLNSYKKKAIETGVASATGDLIVTTDADCLPSPDWLTTIAAFRQEKQSVFIAAPVVF
                     TNDNSLLQIFQSIDFLVLQGITGASVFKNSHSMCNGANLAYEKTAFDKVNGFTGIDNI
                     ASGDDMLLMHKIWKHYPDRVHYLKSKAAIVTTQPMKTWKTFFNQRIRWASKAKNYDDK
                     RIIAVLLVVFLFNLSFLVLAVVGFSCYQYWLYLAGLWILKTIIELPFVRSVASFFNKR
                     ALLNYFFFFQPLHILYTIVSGLFGQIGKYEWKGRRVN"
     gene            42956..44041
                     /locus_tag="PLO70_02720"
     CDS             42956..44041
                     /locus_tag="PLO70_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73394.1"
                     /translation="MSQSQSFQAAAWRRLKKNKGAVFGLIVIILAVLVAVFAYFIAPD
                     PSPYANRIILEIGGEKPGYKQPFIKIKKDKDVVTTSFVTQLVSGKEDVYYYVPIVNYE
                     QKEDSIIVQKYIDEGVNERLAYHNSQTAASPVVTNKFLAGTDKYGRDILSRIIVGTRV
                     SLSVGLITVIISLSIGLILGSLAGFYRGKMDDVIMWFINVIWSIPTLLLVFAITLALG
                     KGFWQVFIAVGLTMWVNVARLVRGQVLGVRELEYVEATRALGFSNFRTIVRHIWPNIM
                     GPVMVIAASNFASAIVIEAGLSFLGVGVQPPMPSWGLMIKENYNFIITQNPMLALAPG
                     FAIMLLVLAFNLLGNGLRDALNVRGRI"
     gene            complement(44006..45487)
                     /locus_tag="PLO70_02725"
     CDS             complement(44006..45487)
                     /locus_tag="PLO70_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQZ73395.1"
                     /translation="MNTVAKKSKALFAFFAGFFVLAIAVSAFLDQYLFIVIPFATILF
                     YAGWENRNTIFFLLLLTLPLSFEYNFSSTLGTDIPDEFLMLLTSCLFLAYWLFSPGVV
                     SKKMRCHPLLLLLYAAFVWQMIAVLFSTNLLVSTKFLLAKTWYISAFVLAPLLVFSTK
                     KSIKIAAQLVAGSMLFIAATALIRHANSGLSFATINDAVAPFFRNHVNYSAMLVCMVP
                     VLFACRKLAERGNLKWVLGVVIVIALVALFFSYARGAWLALMIGILSYWLIKKKLLVV
                     AYISFIIVTIAAVFWLKSNDRYLHYAHDYQTTIFHKEFKEHLIATYKLRDVSTAERFY
                     RWIAGVRMIKDSWLTGYGPNTFYDNYKPYAVPAFKTWVSNNKEHSTVHNYFLLTVIEQ
                     GVPGLLLFIILLGGMLYYAQKLYHRVQDIFYRTTAITIGIILTMLIVVNFLSDLIETD
                     KIGSLFFLCLSLLIIIDINAGSGTSIVQTDERSDPSTDVQCIS"
     gene            complement(45484..46296)
                     /locus_tag="PLO70_02730"
     CDS             complement(45484..46296)
                     /locus_tag="PLO70_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73396.1"
                     /translation="MPDITELIKKWWKQILAVVIVSLLAVSIIVFSKPLQYVSVVTAV
                     PASSYSSDRSKIFNENVQQLYSTLGTPDDLDLIVGTGNLDTVYLTVAQQYNLYDHYKM
                     NEKGDAAINKAASILKKNTKVMKSEYGELKVKVWDTDKNLSPQLANAIMDQLHSIHQH
                     LQSAGNEATLRGLLSGKEKIQQQLDSISGTIPENKMVLQNKLMEYEKLIGEFQLMVDS
                     KPPVIIPVERAKGSDWPDRPKRMQILIATAVLSFLFGLFLALILERRKNTRQ"
     gene            complement(46311..47774)
                     /locus_tag="PLO70_02735"
     CDS             complement(46311..47774)
                     /locus_tag="PLO70_02735"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73397.1"
                     /translation="MNIMLMKETPRFYSSLGLLIILNIIIKPVWIFGIDRSVQNITGT
                     EVYGLYFSLLGFSIVFNFLLDWGLSAFINLELAAAKESHSNIAGSFVAIKLLLVILYV
                     VVVFFAAFISGIGQWDIFWSVVVIQVLTSLFLFFRSIITAQQWFRTDAWLSVLDKILM
                     IIVCGSFLLFPSVTGTIDINLFLLIQIGCSAIVLLISIVVLLRKGFIFSLPSSPLFNK
                     RIFKSALPFGLIVLLMSAHYRLDGFLLERLHGNGAYETGIYAGAYRLLDAANMPGYLT
                     ASFLLPFIARQYSLKKDMSEAILNSRHLLLVFSLMIAVIATILSPWLQEILYHDQDAE
                     AIEVLQWCLPVLIAYSLVQVYGTVLTATGHIVPFCYIVLASVVTNVLLNLLLIPDLGA
                     RGSCFAALVSQGFCGIGTMLYTRQKLGINIHYRSLLMYIFMGVLMFGFLYSGNKYILN
                     KWWLLAGLFVISITASIILKLSDIHKWKTFLKQTTNN"
     gene            complement(47802..48266)
                     /locus_tag="PLO70_02740"
     CDS             complement(47802..48266)
                     /locus_tag="PLO70_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HQZ73398.1"
                     /translation="MASKLNLDKLDLQIIHHMMENAEISYADLGKKLFVSGGTIHVRI
                     KKLQEAGVVKGTRLKADLKQLGYDVIAFIGIYLKESSLYDTVAKDLQKIPEIVRLNYT
                     TGNYSMFAEIVCKDITQLRYVLHDQLQKIKGIERTETFISLEESFSRNVKVM"
     gene            48743..49978
                     /locus_tag="PLO70_02745"
     CDS             48743..49978
                     /locus_tag="PLO70_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQZ73399.1"
                     /translation="MAITLKVLPWLYRSKTNAEGKTPIYLRMTCGNMRVEVATGYFIK
                     ESEWNTRSHTVNSKAPQARQINEHLQALRSKAFQLYNQLVAQEKPFTLAVIKNKLTGK
                     DKNQTTLLNAIQYHNELVKKGIGRNYAIGTYKGYLWFTDKIKSFLMYQYKRSDILLAE
                     LNHRFLTEFEHYLYHHLNNQVNTVQKNITQLRKIINLCIDLDWLDKMPFKRIKARPYT
                     PKRDYLTKEELETIQNIEISNKRLCTIRDKFIFQCYTGVAFIDLNNLTASNVVKGIDG
                     EDWLILNRQKTKSRSTVPLLPIPKALINKYRDETRETLFPICSNQKFNCYLKEISLLL
                     KINKRITTHTGRRTFATSVTLSNGVPIETVSRMLGHCDLKTTAIYAKVVDTKVSKDME
                     RLKHELDGTDIKKARNGSS"
     gene            50035..50943
                     /locus_tag="PLO70_02750"
     CDS             50035..50943
                     /locus_tag="PLO70_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73400.1"
                     /translation="MPSRFTTILEFLSVQKVLLEKVRILEEVSENPDNITNIDRQNVR
                     ITMLHLDDEGKHFEETYSLDDMLLSEFKNFITKKTVDELRVLKFGSKEGILPIKASDY
                     VNTLKNLETTIRQGVKQLQESGLPIPVTLAEITRFCKLLEKRKLEFIDEQVSSQPIND
                     NYPFRGYRFTDPKIKKPLELLDRIADYLIQENLIGPNRKTSFISLFTESWDQTRIPWK
                     GDPKEFFCFYATLFCSSRFQKKPLIEVKGNKLKKEQLLDYCFLVINPETDKPMSKVEL
                     RNYESKPKSNKVYKGIADIIEDLLDI"
     gene            51179..>51912
                     /locus_tag="PLO70_02755"
     CDS             51179..>51912
                     /locus_tag="PLO70_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73401.1"
                     /translation="MKVFINQNGGYNIKGSVWKYQLGNNITPLELPAYEEAIKDLNSL
                     TFGTYMESKVISYEFGLNIKTTHAPLHYMMQLVSSKLNNRSIDFSPQFSNGNLWCMRS
                     TGIERYIKFYDKTCEAKLLTNLLRIEYVVKSPKRILRANITVKDTLSESFIHYNCDIL
                     RQFTENMIFKADYDPAYLGSLKPRDMWRYLAYMMSKQHNPRLLISTLGLDPVSKSREY
                     KHLKRVIGAHTEGELKKEICEKVNQAL"
BASE COUNT        15858 a        10961 c         9826 g        15267 t
ORIGIN      
        1 ggcactacag aatatttctc caaatcagta ataccttctt tggccaatac ttgttcatcc
       61 acgaataagt tgccggtaca ttcgccggag ggttggcgca aaatataata agcagcatca
      121 gccagaatgt ctgccgtgcg gctcatattg gccagcatgg tgccgcctaa caaatttctt
      181 acagccgccg tatcaatagt ggtccttggc cacagcgcat tggcggctat cctgtctttg
      241 gccaattcct tagcccagcc aatcgtcatc atgctcatat tatactttgt aatagtatag
      301 gccacatgct tttcaaacca tttgggatcc atattaatgg ggggtgataa agtaaggata
      361 tgcgggttag ctgctttttt caaatgcggg atgcaggctt tggttacaaa aaatgttccc
      421 cgcacattga tagcatgcat caggtcaaac cgtttgggtt ctgtttgctc cgttgtggtt
      481 aatgaaatag ccgatgcgtt attaaccagg atatcaatac ccccaaattt ttctatcgct
      541 ttatcaatca cattttgcac ctggtcatca aagcgtatgt cacattgcac cgccaatgct
      601 ttaccaccgg ctgcttctat ttcagcagcg gctgaaaaaa tggtacctcc cagtttggca
      661 ttttcttcca cgcttttggc ggcaataaca atattggccc cttcttttgc caatcgtaaa
      721 gcaatagctt ttccgatacc acgggtggca ccggtgatga tagcagtttt gttctgaaac
      781 gacatatagt tcacttttta attaattaaa cggtacagtc cggtaaatac tcagtttgtt
      841 ttaaggaagt ttaaagtctc tcctcctgag cgtttcagga aggctttagt gctctggcaa
      901 aactggttta gtttgtacga tatcttcaat ttgctgcata acagttgcat ctaatttttc
      961 aaccgctttt gctgctttca gattttccag caactgttct tttttagtgg cccccaatat
     1021 cgctgttgtt acattcggat ttttaataca ccaggctata ctcaattcag ccatggagat
     1081 gcccaattct tttgccaatg ctgcaaggcc ctttacttta ttgagcatat cgtctttcat
     1141 ccagcggtct ttcaaccaat caaacccggt gagaccaaac cggctgcctt cgggaatacc
     1201 atcattgtac tttccactta ataaaccgga agccaatggg ctccagatag tagtacccat
     1261 gccaacggtt ttaaatacca tcaggaattc attttccatt ttatttcttt cgagcaggtt
     1321 gtattgtggt tgctccatcg agggaccgat caacccatac tggttggcca cccggtgggc
     1381 ttccatgatc tccaccccac tccattcact ggtaccccag tataaaattt ttccttgctg
     1441 gattaagtta tgcattgtcc aaaccgtttc cacaatggga gtccctttat ctggccggtg
     1501 acagaaaaac aggtccaggt attccatttg taaccgttgc aatgcttcat ggcaggcttc
     1561 gaccaggtgc ttcctgctta atcctgtctg attgggttta ttagccgttc cccgccagcc
     1621 ccagaaagct ttcgatgaaa cagtatagga ggtcctgtcc caatttttct ttttcaacac
     1681 ccttcccatc atcttttcac tttcaccaaa tgcatatact tctgcattgt caaaaaaatt
     1741 caccccgtgg tcatacgcta tgcccatcag ttcatcggcc atgctatcat caatctgttt
     1801 gtgaaaacta acccagcttc caaaagacag cacactcagc tgtaagccgg tcttgcccat
     1861 ataacgatat tccatacaaa aattttatta ttaaatactg gaacgaaggt aagcgaagat
     1921 aataacagaa atgagcaacg atcgttgtta ttgaggaaag ccgttttctg acacttttaa
     1981 attaccggtc gtctctttga acatgatcct cttgagatcc tgtgtagctt ccatcccctt
     2041 tccaccataa atatttttac ctacgccatg ataggtcgca tacttatctg tataaaacat
     2101 tttagtgctc tgatcccacc agagatcagg tgatttgaga gtgtctccct taaccgttat
     2161 cactaccaca ctatcccgca ggtacacttt gttcaggttt tcgtagtaga taccatactt
     2221 gctatccagc caagtctcta attgggtgct gtcatcataa aaatcacagt gcaacttgtt
     2281 ggggaattca actgagatcg tatcaccagc caccctgatc atcaaaggag ccttcaattt
     2341 tgcttttaac tttccgtcct ggctcaggta actttctacc tggatggctt cttcctgcat
     2401 caccactttt ttggtccagt cctgcagcac tttatcatcg ttttcacagc cggccataaa
     2461 aaaacagcct gtaatgaaaa aggctgtttg aataattttt ttatggacag aaaacaataa
     2521 cattattaac agggtaagtt aaacccacca aaataactgt ttaatcgtat ttgcgtttta
     2581 caaaccagat atcgctgagg gagaaaccta atgagaaccg gaacatgttt tctcttaaca
     2641 ggttatcatc attacctctt ttgatatact caaacgccat attgataaag gtgatctggt
     2701 taggtgcctg gcggctgata ccaaccggca accctaatcc gaatgtacct ccgatttgcg
     2761 gaagtttttg tcccactttt acataatcag gtcccataaa gaaaccaaag cggtaggcta
     2821 tattactgaa ataatttctt ttgggaaccg ggctcagttg tgcacctacc tttatctccc
     2881 atttattttg caacgaatct gcctggccat agaacctgta ctggctccag ttttgctggc
     2941 tgaaatccac tccgaacaac cagcctcctt ctttattagg cacggcatat ttttgtaata
     3001 caaacccaaa tgtgtaagaa gcgggaagtt caacagtacc ttttatatcc ctgagatcgg
     3061 acacactgtc taaccttaca ttgcccaaac tctcgtcata gataaatgtt tctctcagtt
     3121 tatcctgcct gccattcatc ttttgtgccc agttgccata ggcgccgatt gtcaataaca
     3181 ttttagtatt caatggaagt ttatactgta caccggcatt gaaatataaa ttaccgtagt
     3241 tggttcttgt ttcataattg gtccggtagt aactaaccgt atcgttcaac aatgttctgc
     3301 gggtactgta atccttttca ccaaacaggt agccaccgtt catgcccaca ctcagttttt
     3361 cttccagtcc tttcctttcc cggcgaaaca ggctgaagcc ggttccaata gaggcgaggt
     3421 aagaaccacc atccccttca aaaccggtaa aagcgctgtc aattggtaaa ccggttgtcg
     3481 gatcaatcaa cctttcattg cgaaagatct tatagctgat ccttgaaaca ggccttaaac
     3541 cgaaactcaa accccagttt tgtttcaacg gaacacccac ctgtacataa gaaaacaagg
     3601 cattactggt agtaaatttt tgtgttactc ccggctcccg gagtgtacga ttttcaaaat
     3661 tcaggcccag gtccaggatg gctctgccgg agctgagttt tttggatttc ttttctttat
     3721 aggattgaaa tgaagaatac gaagcggggt tattgaaatt gatagccagg tggtctatat
     3781 aaccggcaga gagaccagcc ataccccggt tgataatatt agttgacggg accaggtcgc
     3841 cgatgccata ccgggaataa ggtgagttat cttgtgaaaa tgcaggggta acagcagcca
     3901 ggatcaaaat cacaaacgtc cactttttgt tgtgtaacgt attgattatc ttaggggtta
     3961 ttaacttcac taattgcata cagaccttta aatattaaat cagggtctgc aaatatcttg
     4021 tttttaaggt gtgaagccaa atagactata tcacccccgg ttaaaaggac gttaatgttc
     4081 cggtactttt cccggtaagc ttctataaaa ccatccatct ctttggccat acctaacacc
     4141 accccggaaa gaatattggt gttagtatca tacccgatca atggcacatt gctgtccgcc
     4201 tttattaagg gaagtttagc agtataatag ttcaatgctt tcaaccgcat ctccaatccc
     4261 ggtgaaatag cgcctccaac aaattcatgg tatttattaa tgaaattata agtgatacaa
     4321 gagcccatac caattaccag gttattatag gtgggataat aatgaactgc tgcagccgtt
     4381 aatgcaagcc tgtcggcgcc gatagtttcg ggtttgccca ccggggtagt aaatgccact
     4441 ttggtgagat ggctcagctt gtgaaattta gtagtcgcag ccagtaattc ttctattaaa
     4501 ggattatgat caataacaga agatagtatg gatttacctg gtttgaaatt atttataagc
     4561 gattgtatcg tttcgataga atcatccggt aacacctgcg cttctttcat ttctgctcca
     4621 tgaaagacgg ctactttttt ccgggtattg ccaaaatcaa aacaaagggt tactgacatg
     4681 aataattttc tttaaaggtt catttctatc accattcaaa cccacccagg tttttaaaat
     4741 gtccgttgag tttaattttt tcttttagct gttccatccc ggccagcaca ggtatggctt
     4801 tgatcagatg ccgtttcagg tattcctgtc tttgtctttc atcggctaag cacagcaatt
     4861 catattcttc ctccagggat aggccgatat gatgggccac atcataactg ttcatttttt
     4921 catcctcctt cttaaaatcc ttggtcactt tcaacaaacg gtgcaggtca cggatactgc
     4981 tcatcacttt tcgcattagt tccgggttac ctgttttata attatccggg tacgtaacaa
     5041 tagcaccatg gtataattta tccggtactt cttttattat ttccagtatg cgaaaaacct
     5101 tcacccccct tgttttaata tccatttcac cattttcatg cactttcgac aactcggtta
     5161 tttccaccag ggtgccgtac tctttcattt tgttatcgat gactgcagga ataccaaatg
     5221 tcttttttgt tgcatgacat tcactgataa gttgtttata ccgtggttca aaaatatgca
     5281 ggtgaaggct ttcacccgga tacaccacaa taccaagcgg aaatatggga atgaaattgg
     5341 tcatgatagc aaagttaatg ggaagaagga agaggtgagc aggtctaaaa ctgtgtaatc
     5401 gtcactacct gttttcacct cccttgttta actccttaag tctcgcatat ttcagaaaag
     5461 tataataagc tgtcattctt gcacagatat acccggcatg accatccagg aaacccagtt
     5521 tcaatatata ataattgaaa aatgtaaaac caggagataa gcggagtttg aaccaggaag
     5581 ctttttttcc ctgcttataa tatttctccc cattcagcat cgcatacttt accatcttgg
     5641 cagcataatc atgtatgtcc ttcattgtct ggtgcagtac atatcctttc agctttttaa
     5701 taacagcatt tgccggtatt atcaattgtt catgtaccgg tgcttcatcc cactgaacaa
     5761 attttttatt gaaaaaacga atatgatggt cgccacccca ttcaccatac ttcaggtatt
     5821 tttcaccaag gaaattcttg aataggatat catagactat aacatcattc ccaggattcc
     5881 attgtaataa tgattgtctt aattcttcat ctgcagcctc atcggcatcc aggcttagta
     5941 tccagtcata ctttgccagt tggttggctt ttgtttttgt tttaccaaat ccttcccagc
     6001 taccttcatg caaatgcact ttgaattgcc tgaccaggtc ttgtgttcca tcggtactgc
     6061 cattatcata cacgataata tcgtctgtaa gaccctgcag gctgcttagc gtcttagcaa
     6121 tagcacctgc ttcattccgg caaataataa caatagaaaa ttgggacata gttatgcaaa
     6181 gattacacct tttactattt caaccatttc caccaccata aaacaataaa tgaccaggta
     6241 aacaccccaa actgcacttc cagcccgata tcaaacaaaa aactgaaagc agctgtagcg
     6301 ttgagcaacc accaaagtat tttgtctttc acctgaacaa agaatggaat aagcatcaca
     6361 acagtaaata atacaaatcc cggaatgccg ctgccggcac catacatcat ccattcactt
     6421 cccgggtata atttatcctc ttcccgcatg ccaggataat gagaagcata ccatagcctg
     6481 gtttctgtta atacatctcc aaacccggta ccggtaacag gattttcttt cataatattc
     6541 caaccccctt taatagagat cacacgaagt gcatcgtttc ctcccgggag gtaattagcc
     6601 ttactaaaat aaccgtaatc gtaattaata tattttatcc tgttttgaaa tgttggcaat
     6661 gtaaaataag ctgtaaccgg cattgcaata agcagaatca gcaatataac actgtacccc
     6721 ttttttctgt ttttaatgat catccaggct gctgtcataa acagcatgct ataaaatgag
     6781 aaaaggccgg tccgcacggc aagaatatgc agaaaaacag ccagccatcc cgccactgta
     6841 atcaaaacca atgaagtcgt cctttcggac ttttgttttg aaaatgccag ccacccggca
     6901 agcaatgctg ctatacttac cagccagcta aaccttaccc tgtcattctc cagtggtgta
     6961 aaaaaggttt tagccctaag atatccttca tgtataacgg ctgtatcctg taaataatga
     7021 tacatacacc ataaagtacc gcctgtaata agagctataa aaatataccc cagccactcc
     7081 cattgttttt ttgtaaatga aaaaggactg gcaaaagcta taggaagaaa caaaagcggc
     7141 aatttaatac ggatcatatc cagccattct tttttatcac tgctccatag tcctgaaatc
     7201 aagggaatta aaaacaatac actcatcccc cataataatg gagtactaaa aaaatcatag
     7261 acctgctttt tactattact atgtaaaaaa gaaaccagta taaatactat catactggca
     7321 gaaagaagtg cacgggaaat aaacaaagcc cctgtcatta caataaacag tataaaaatg
     7381 atagtgtttc ttctttcatt ctgtaacttg ttcagcatac ttttgtaaac gtacaacatc
     7441 ccgttcattc tatgtggcag gaattattaa agcaaataca gcaaggtgat accaaagcac
     7501 tggcccgcag tatttcattg gtagaaaatg aaaaaccggg ttatgaaacc ctgcttcaat
     7561 cacttcctgc cagtacaacc aaaattatcg ggatcaccgg acctccgggt gccggtaaaa
     7621 gcacattggt ggatgcatta ataggtttac tgatcaatac tggtaagaaa attgctgtac
     7681 tctgtgttga cccatcttcg cctttcaatc atggtgctgt gctgggcgac cggataagaa
     7741 tgagcgactg gtacacccat ccaaatgtat acatccgctc attagctaca aggggttcaa
     7801 tgggtggcct gcatccaaaa atcattgaga tcactgatct gctgaaagca tttcagtttg
     7861 atcatattat cattgagacg gtaggtgtgg gtcaaagtga ggtagaaatt gctgggctgg
     7921 ccgatgtaac ggtggtggta gtggtaccgg aagccggtga tgaagtacaa accatgaaag
     7981 caggcctgat ggaaattgca gatgtatttg tagtgaataa agctgaccgg cccgatgcag
     8041 atattttcgt caggaacctg agatcaatgc tggctccttc ttttagtaaa acttacaacg
     8101 aagtacccgt tataaaaacg gtggcttcac aaaaagaagg tatcagtgag ttatcagcta
     8161 ttattctcga tcttttacaa aaagcgcagc tgaatgataa aacattctgg ctgctggcag
     8221 aaaaagctta ttacctgatt gaacaaaaga ggatgaaaga cgtcagcaag gctgagctaa
     8281 aaaaagaaat tgaagtttgc tttaaaaaag gaagttttaa tctttataaa tttatagcgg
     8341 ataaataact gactgtactt aaacccaaaa gcctgatact atgaaaaaca gaaaccatat
     8401 tgttaaaggt ttcattgttt tgtgtgtatg gctgacattt tttttgttgc ccgtttccgc
     8461 aacagcccaa acaacaaata tcagtggtat agtaaatact tatcacaggg tggtggaaat
     8521 aattccatcc aaagcctgta taagggttgc ctctgttaca ggccttacca ttaatacaaa
     8581 agtagtgctg gtacagatga aaggtgctac gatcaacacc agtaactccg ctggttttgg
     8641 aactgttact tccttaaacg atgccggtaa ttatgaaatt ggcactatat gctctatcat
     8701 tggtgattct gtttttttgt ttcaaaattt attaaatact tattctcccg ctgcgggaaa
     8761 actgcagttg gttcaatttg ctgaatatta ctctgccaat gtgattgata cggtaaaagc
     8821 tgctgcatgg aataatacaa caggcaccgg tgctgtgatt gccatctatg cagaccagga
     8881 cattacttta aatgccccgg tttatgctga cgccagtggt ttcagaggag gaaactttct
     8941 tcaaagtaat ggaacctgca gcgacgcaat acccgccagt ggatatatct atactgcatc
     9001 aaataccagt cctcaaaatg gtgcctataa aggagaaggc attgcagacg taaccagtgg
     9061 acaaagtggt ggccgtggtt cgcctgccaa tggagggggt ggcggtaata atcataacaa
     9121 tagtggtggc ggcggagcta acttaacgac aggaggaatt ggtggcggaa attccagtaa
     9181 tggcgtagcc tgcacagcca ctctcagggg cgaagcagga aaaagtttaa gcagcaatag
     9241 cgggcaaaaa atttatttag gtggtggtgg tggcgccggt cattccaata atggactttc
     9301 aaatgtatct ggtggtaatg gcgggggtat tgtttttatc tgggctaacc agctgattgg
     9361 aaacaacaga ataataactg catcaggagg caatggcggc aattcattat cggatggtgc
     9421 aggcggtggc ggtggcggcg gcactataat aatgcatgta agcaattata ccggaaatgt
     9481 aactgtacag gcaaatggtg gaaacggcgg aaattctgat gacggaggaa atatcggtcg
     9541 ttgttttggt ggtggcggag gcggaagcgg cggtgctatt tattttacag gagcgctgcc
     9601 ggcaattact actgacataa ctggcggagc cgcaggtgtt gaatcaggaa gagatgtatc
     9661 ctgtgatgct ataccgcagg cagggggggc aggcagtaac ggccaggtta ttactaacta
     9721 tactttttca agatctacca atcctgctgg ttattgtgcc tttcttttag catccggact
     9781 taattatttt aatgccgtcc gcatgggcaa tcttgttttg ctgaattgga aaaccgccag
     9841 tccggcagaa atacaatact ttgaagtaga aaaaagaaat gcggccaaca gctggaatag
     9901 tatctccatt gttagcggaa atgatgccac cagggaatac tctgcaactg ataacaaccc
     9961 gcaacaaggg tataattatt acagattaaa aattaccgga agaaataatg ccgtttcata
    10021 ttctgaaata aagagagtgt atatccggca agctggtgct gaatttgaga tatatccaaa
    10081 tccggctatc agtaagatca ctatcagcag aaaaaatagt ttaccggggc aattaaaaat
    10141 aacggatata accggcagga taattcatca acagacaatc tcatccggtt ctgccgaaat
    10201 caatttacca actatagcaa aaggcattta cctggtaata atcgatactg tagtaaagaa
    10261 gttagagatc aggtagaacc gttattcgtt cttattattt ttccaccagc ggtaactaat
    10321 gagaccaact atggcaaaag gtgcaaaggc gaggtaaaga ataccagaat tcattccctt
    10381 ggcgggcttt tcacctaact gttgggctgt tttggtacaa acagagcatt gcgcctgaga
    10441 atcaagccct gtgaagagaa gcagagaaaa aaataaacca atgaccacga tccacttttt
    10501 catacccggt gattgagctg taaaaataca acgaaaatca ttgggtttgg ttcaaaaaat
    10561 caacctcctg caggcggact gcatagtttt ttgatcctgg catcggcatt tttattaccg
    10621 gctttctttg ctttttcaaa atcagcgcag gcttccgttt tcttttccag cagttcatag
    10681 caaatacccc ggtaataata tacattatca ttagcgcctt tggcaaattc cagcactttg
    10741 ctgtaatcag ctattgcctt cacataatct ttccgagtat gatgcaggac acctctttgc
    10801 cagaatccgg ctgaatcctt tggataaaat tttatatatg tatcatagtc agccagtgcc
    10861 gctgtcgtgt ctttatttgt taaccggatc ttggccctcc aggtcagggc atctttattc
    10921 ttcggttgct ttttcaacat ttgtgtcata tccattagtg ctgaatcagc ttttttcaaa
    10981 ttactgtaaa cgataccccg gtataaatac acgctgtctt cttttatatt ttgtgctatg
    11041 gctttgttaa aggcaacaag ggcctccgca ttttttttat aggcaaacaa tgcccggcct
    11101 tttacatagt ccggaactgc ctcactcatt tttatagtat atgtataacc cgggttttta
    11161 atggttttat tctcgacttt accaaccatg atctttcccg acgggtttaa aaggaaacct
    11221 tctttgggtt gtatttctgc aatactgcca taatatccga tctcattgat actgccaaag
    11281 aaaacggaat tatccggaaa ccagatctcg aagaatgaac catcagagaa ggtggtccgt
    11341 cgggcatagg tgccgtcttt cagtatccag ttctttgcag aggaataagt ggaagtacca
    11401 tcaaaacttc cttcaaaaaa cagtttatcg ttacggtaaa attttccatt gcctgacggg
    11461 ttgccggcaa caaattttcc ttcgtattta tttttgttcg gccagctcca tactccggtt
    11521 ccggcataca ccccattgct ccagtcgcct tcatagatag ttccatcctg gtagatcttc
    11581 ttccctttgc ccgacatcac atcacctcta aaactgccgg tataactatc cccgtttttc
    11641 caggtatata caccttcgcc gtttatttta tcatctttcc attcgccaac atatttatca
    11701 cctcccttat aaattttggt gccggtacca ttcgcctttc cgttcaccca ttgtccatca
    11761 taacttgaac tatctttcca ggtacacttc ccgttgcctt cccatttacc atttttccac
    11821 tggccttcat atttttttcc atccgcatac acaccaacac catatccttc tgcaagacct
    11881 tttttgaaat ccccttcata cctgtctttg ttccctaata ccagcttacc ttttccctct
    11941 gcctgtcctt ctgcatattc accttcatag gtctgtccat ctaatttggt gatgtatttc
    12001 ccggttccac ttcttttgcc atctttaaaa tctccggtat aaatatcacc gttattataa
    12061 acatattttc ctttgccatg ctgcttatct gccatccatt cgccttcata cgaactactg
    12121 tccttataaa ttctttttcc atacccttca aacttatcat ccttccaatt gccgtcatag
    12181 cttcccccat cgggccaggt gaattttccc ttccccgacc ttactccttt taaccagctg
    12241 ccatcgtaga tcctgccgtt actataaaag tatttaccct gcccgtcata cttacctact
    12301 ttccaatagc catcataccg gccactgtcg gcagcataaa aaataccata cccttcaaaa
    12361 tagccatcct ttacttcccc ttcatacctg cggccatcct tccaaaaata ttttgctttg
    12421 ccattccagt ttccgtttat ccaatcacca tcatacagac taccattaac ataactcatt
    12481 ttccctttac catgacaata accgttttta aaataaccct catacacatc ttcattttta
    12541 tatttgaatt tcccaaatcc atctttgcaa tcgccactca ggcactgggc aacagtaaca
    12601 gaggagatca tgctggcaag gaaaacaaaa aggatccggc tatacataat ttaagtttgt
    12661 ataaaagtag atttatcccc gctgccggta aataccctgt ggctggtata ttcatcccaa
    12721 agcagggtta taaaacccgc tgtgacagga ataaaaaatg ccgcccggaa aggcggtatg
    12781 gtataaaaat cattagctga ataaacggcc tcagctttcg ctttcttttt attggaagga
    12841 gactttccgt atcctgtgat tagcattatc agcaatataa atattgcctt gaatatctac
    12901 tgcaactcct ttgggatagt aaaattttgc cgtattaaat aaaccatcag cgaaaccttg
    12961 ttgtccggga gaaccagcat aggttgtaac aacagctgta gttgaaattt ttctgattac
    13021 ggcatttgtt acatccccaa taaaaatatt accagctcca tcaatgcaaa ttccgtttgg
    13081 gttagcaaac tctgccatta tcccaatagc atttaaaaaa ttattagcac cgctaccggc
    13141 aaaggttgtt actgttccat ctgatgtaat tttcctgatt ctgttttgat cagtaacata
    13201 taccacccct tgattatcta cagctatatc ctgcaggtta tagaattccg ctaaactact
    13261 tatcccgtca gaatagcccg aagtgccttt gcccgctaat gtgcttactg ttccactagg
    13321 agtaattttt ctaattctga aattactttt atctgcaacg tatacattac cttgagcatc
    13381 aacggctaca cttatgggtt cccaaaacct agcgtctgta cctgttccat tttggtatcc
    13441 aaacgtactt ccacctgcca aggtagatac ggttccgtct gctgtaattt ttcggatttt
    13501 attattgtca cgatccgctg cgtaaatatt cccttgtcca tcacaggcta ttcccatcac
    13561 cggattaaac tgtgcgatat tacccggacc atccaaaaat ccccttatcc cagatcctgc
    13621 taacgtattg acaattccgg ctggggtaat ttttctaatc tggttatagt ctgacacata
    13681 gatatttccc tgagcatcca gacaaatatc atatggaaaa ttaaactgtg ctgcactagc
    13741 gttcccattc aacactgcct gtactcccgt tcctgccaaa gttgaaacag tcagattagt
    13801 taaaactgta aatgaagagc cattaacagt tctgttattt actgttattg ttatagcacc
    13861 gttggaagcc cctgccggaa ccgttacaat tagtgtatct tctttagcaa cggaaattgt
    13921 tgctattacc ccattaaatt taacaatatt tgataagatg tttgtactga aattatcccc
    13981 tttaattatg acagttgtcc caactcctcc cgacaaaggt gctattgaaa aaataaccgg
    14041 atcagaatta acaggaacag gtgcaggagt gttttcccct ttaccacatg agattgctaa
    14101 tatgcaaata aataaagaga ggcattttat tttattcaga aagtacatag tcattcagtt
    14161 ctaatactat tttgatataa tattaaattt atgactttcc cctgattttt aaaaatcggg
    14221 gaaactgaac agaggagaat actaaacagc ttccgtttcc atcttcttct gcactttctt
    14281 tctttcatcc tcattcagga tcactttccg catacggata aaattcggtg ttacttctat
    14341 gcattcatcc tgctggatat attccataca ctcttccagt gtcatctgtg ttttgggagc
    14401 aatacgggtg gcgtcatcac tgccgctggc ccggtggttc gttaatttct ttggctctgt
    14461 agcatttact acaaggtcac cgggtttgat attctcagca atgatctgac cggcataaac
    14521 ttcttctgcc gggtcaacaa aaaatgttcc cctgtcctgt aacttatcga tagaataacc
    14581 tgtcgtctta tcagtattct tggaaagcaa aacaccatta cttcttccgg ggatcgggcc
    14641 tttccagggt ttgtattctg taaaccggtg tgccattaca gcttcgccgg tagtcgccgt
    14701 cagcatttgt gtacgtaaac caatcaaccc tcttgaggga atttcaaact caaggtgttg
    14761 catttcgcct ttggtttcca tgatcagcat ctcgcctttt cggcgactta ccaggtcaat
    14821 tactttactg gcgaactcct gcggcacatc cacaacaaga ttttcgtaag gctcacattt
    14881 tttaccattg atctccttta caattacctg gggctgacca acagttaact catacccttc
    14941 acggcgcatg gtttcaatca ggattcccaa atgaagaata ccccggccat ataccatcag
    15001 gctatctcca tcttcactgt cttccactct cagggcaagg ttcttttctg tttctttcat
    15061 caggcgatcc cggagatgcc ttgaggttac aaactttccg tctttaccaa agaaaggtga
    15121 gttattaaca gaaaacaaca tattcatcgt aggttcatct acactgatca ccggtaatgc
    15181 ttcgggattt tctgcatcag caattgtatc gccgatatta aaatcttcaa tacctactac
    15241 ggcacaaagg tcaccagcca ccacctcggt tactttcttc ttacccattc cctcaaacac
    15301 atataactct cttactttcg tcttttttat agtgccatct gcctgcatca ataccacatt
    15361 ctgtccttct tttaaaacac cacggtttac tttacccaca gctatacgtc ccaggaaaga
    15421 tgaataatcc agtgatgtaa tctgcatctg aacaggacct tcattcactt tgggtggtgg
    15481 tacatgtttc aaaataccat ccagcaaagg gttgatatta tcaatctgct ccaatgaatc
    15541 attgaaccag ccgtttttac cacttccata atacgtaggg aaatgcagct gctcttctgt
    15601 agcatccagg ttaaagaata attcaaatac agcatcatgc acctcgtcag gacgacagtt
    15661 aggtttatca actttattga ttacaactat cggatgcaga ttaaggtgca gtgctttttg
    15721 taacacaaat cgtgtctgtg gcattggccc ttcaaaagca tctaccagca gaataacacc
    15781 atctgccatt ttcaataccc tttccacttc accaccaaag tcggcgtgac ccggagtgtc
    15841 aattacattg atctttatgc ctttatatac caccgctgca ttcttactaa aaatagtgat
    15901 acccctttcc ctttccaggt cgttgctatc catgatcaga tccccggttt cctggttatc
    15961 ccggaatacc ttggttgtat gtaaaatctt gtcaaccaat gtagttttac cgtggtcaac
    16021 gtgggcaatg atagcgatgt ttcttatttc cattgtgtta agttgtgagt taaaaagttg
    16081 acaggttaat aagttacttt tctttagctc ttttttccaa ataattgata tacccgttta
    16141 acaacttttc aacctctgtt attttgattc tatagtactt taattgttct tcactgatca
    16201 gtttttcatc caaagcatca atcaaatgat ttaaggtttc acttaatgat cctctggcaa
    16261 taacacagaa cctgatttta tccggccatg tattacgacc atgtccctca gcaatttgtg
    16321 cattgatgga ccgggagctt ctaatcaact gatccgaaag tcgtaatcgt tcgttcgtcg
    16381 gaaaagttcg taccagctgc gcaatttcat tcttaaaatc tcttgatttt ttccaaactt
    16441 caagttcttc aaagctttga tatgccatat tttaaaagtt aagtgactga atctgaaggg
    16501 atctccttat caacttctca acctttgaac ttgtcagctt ttccgggcgg caaaggtacg
    16561 ttattttagc cgggggagca aacgaaagag gtgtttaatg cagtcttaac ctgttaaaaa
    16621 cagagaatta atgtagtttt atcaaaacta caagatgaaa tttctcaaat ggctaggtat
    16681 catcctttta gttcttattg ttatttactt tttaggtccg cggcctgcca ccccaaaata
    16741 tactaatgac ttgcctgcta ttccagcaga gtcctccatg cttgagcaat atatcaggaa
    16801 taatgaagcg aaacataaac tgaaaccaga taatgaagct agaatattgt ggttaaatga
    16861 ttcaataaaa gcgaaaacag aatatgccgt cgtatacctg cacggttttt ctgccagcca
    16921 ggaagaaggc gaccctgtgc attacgagtt tgcacaaaaa tttggatgta atttatacct
    16981 gagccgttta gatgcacatg gtatcgatac cacagaaccg ctcggtaatt ttactgctga
    17041 gggcctttgg aattcagcta aagaggccta tgcaatcggc aaacaattgg ggaaaaaggt
    17101 gatcttattc tcgacctcta caggaggaac actatcatta aaattagctg cggaatttcc
    17161 cgatatagct ggcctgattc ttttatcacc caatattgca atcaacgatc ctaatgcctg
    17221 gctgttaaat aatcactggg gattacaaat tgcacatttg gtcacaggca aataccgcac
    17281 tgttgaagac actacttcca tttatgctca atactggaat aacaggtatg ttacaaaatc
    17341 gttagtacaa ctggaggaac tgcttgaatc aacgatgaag gcatccactt tcaaaaaagt
    17401 aacacaaccc actcttcttc tttattattt taaagatgag gagaaccagg atccggtagt
    17461 gaaagtgtct gccatgaaaa gaatgttcaa tcaactgggt actcctgata gtctgaaacg
    17521 ccaggtagct atacctgatg caggtaatca tgtattggga tcttatatta aatcaaaaga
    17581 tatcgaaaaa gtgcaggagg aatgtgagaa gtttgcaatg gaggtgctga aaataaaaga
    17641 tcgttgaaat tctcaagtgt ttttactaat aaaatggagt aaattactac catcattatt
    17701 taataaaaaa attaccttca ttatagtaat ttatcctgat gaaaaaacag ttattaataa
    17761 tttttgtggg tctatttgtt tgtagtgcct gtgacaaaga caaaacagac aagccatatg
    17821 ttccgataat ccgatgtcca gatatgacaa ggaatatgga tactattaat aactatatac
    17881 gaggtaactg ggaatgggtt gaggaatata gggtgacaag gacttttcag ggatgggtta
    17941 ctcctaatac acccggagag aatcatttga gtttaaaatt atatggggat accttgcaat
    18001 tttttacaaa caacaggcca gattctttct ataattttcg tatccgtctt ttatccgatt
    18061 taaccaatta cccagatgac tcattaccca taatttatta tactagtttt tatactggta
    18121 tgggaggggg cgttattcca ataaaaatat gcaagaacca gttgttaatg cagcaccagt
    18181 atgtttcaag tataattggt gaaaggttat ggataagaaa atagcagcaa aaaagactag
    18241 aattcataac atgatatccg cacactattg aagtaagcaa ttttaatctg tatcataata
    18301 ttgtaaaaaa taatattatg aataagtttt ttgtatttac agttttgctt attgcatcta
    18361 aaagtttctc acaaggcttt acaaacagat gtggaactat tgcagacatg aatttaattc
    18421 agacgaatac cccaaacgtt tatcagagtt atcttgatat agaagctttc acagccaatt
    18481 atattactaa tcaaacccaa actaacagca gactaattaa tggtgcaggt ctgattgtaa
    18541 tacctgtggt gatacatgtt ttgcataatg gcgaagctga gggaacgggc ttaaatatta
    18601 gtttagcaac tattcaatcg caacttgacg tgttgaatga agattttaga cgcctaaatc
    18661 ccgatgctat taatactcct tctgcttttc aaggggtagc cagcgatttt ggtattgaat
    18721 ttaagcttgc ttgtatagat ccaaatggaa atgttacaaa tggggtggtt agaaaacaag
    18781 tcagtgcgtc acaatacaat atcagtgtaa ggactgatgg aagttatgat gacgatgcaa
    18841 tcggtattaa aaaaagtagt acaggtgatg ccgcatggcc aacagatcat tatttaaaca
    18901 tatgggtatg taaaatgaat aatgggactg ctggctatgg gacttttcca tttcattatt
    18961 caacctatcc acagtttgat ggcattgtta ttgacaaagg tgcatttggt agaaatggtg
    19021 gaacaacatc aggctttgat aaaggcagaa cggccactca tgaaattggt cattggctta
    19081 atttatcaca tttatggggt aacacaatta gcccacagtt taattttgat tgctttagcg
    19141 atgatttggt aggcgatact ccgcaacaaa aaacttataa ctctgggtgt ccttcttttc
    19201 cggaaatcct tcgtaaatgt aatagtgtcg acccgagttc tatgtttatg aattatatgg
    19261 attacacaaa cgaccaatgc tataatcttt tcactaacgg tcaacgacta agagcaagag
    19321 ctttatttgc gacaggcgga ccaagggcta catttctaat taattatttt caaatacaaa
    19381 caccaacaaa ggtctcttgt aatggaagtg ttattaaatt gtttaatcct acctgtgcta
    19441 cacccacttg gtcaatagtt tctggtaacg caactattgt aagcggccaa aatacaaata
    19501 gcttaacagt ttcaacccct tcccccggtt catttgtttt aagggctact ggcggcaatt
    19561 atattgagga taaaacaata gatgttgtac cctttacaag ctcagactat actctagttg
    19621 gtaataatgg ttctatgtat tggtgtccta atcaaaccac ttcgtttagt gtttcagggc
    19681 aaggggcaac gaattataat tggtcgcttc caacagcatg gacaatgatt aacaatgggg
    19741 gtagttatgt agtagtaagg gccccatcca gtacttaccc tccaactgga acactctctg
    19801 ttagtgttac agaaccatgt ggtacaatta tcgctataaa caagtttctt gcctatagca
    19861 gtagtgcttg ttcgccaagt attcctttta ctctatatcc cagcccggca tctacatatg
    19921 tcaatattga agtaaccaac tctcaaacaa atattgcagc agttcaaata acagatatgt
    19981 atggaaacat attgattaat aatcaatatt caggcaatta tactggagta caaattggtg
    20041 tatatgcatt acaaaatggc cctaaaatag ccaaaatata tagtgggaca caatggtatt
    20101 caattcaatt cacagtaaca cattaaaata attttaatag catttacata gtgaaacaaa
    20161 attatttccc aagtttttac caagcagcaa tacctgatgc aggtaatcat gtattgggat
    20221 cttatattaa atcaaaagat atcgaaaaag tgcaggagga atgtgagaag tttgttacag
    20281 aagtattgaa aataaaccag caataattat tttactacta aacagcaagc tatgacatca
    20341 aaatggtcac gccgtgatat attacggttg gcaggtttat cagctacagc gcttccgcta
    20401 tcatcctggg ccactaatga aaatggtgaa agaataatac tgccggataa cccgcagcac
    20461 aaaaaattga aaaaacctgt aacggctatt accctgggtg ccggatcaag agggaatgtg
    20521 tatggcaatt atgccgttca atatcctgac cagcttgata ttattggtgt agcagaaccc
    20581 attgctatcc gcaatgaacg ctatacccaa aaacataata tagcggcaga aaatcgtttt
    20641 caaacatggg aagatgtttt taaaaaacca aaatttgcag acgctattat catatcaact
    20701 cctgataacc ttcactacgg accgtgtata gaagcattga aaatgggtta tgatatattg
    20761 cttgaaaaac ccatttcccc cagcgaaaaa gaatgccgtg acatcttaca attagctaaa
    20821 aaaaccggaa gaattgtggc cgtatgtcat gtgcttcgct atgcgcctta ttttattaaa
    20881 ctgagagagc tgatgaacag cggtgctatt ggagaagtag tcagcataca acacctcgaa
    20941 ccgatcggtc atgtgcacat gagtcattct tatgtacggg gcaactggca taacagcaaa
    21001 gaaacaacac cgatcatttt ggcaaagtct tgtcatgacc tggatatatt aaaatggatg
    21061 gtcaatcaac cttccaaaaa aattcaggcc tttggtgacc tgaagtggtt taaaaaagaa
    21121 aatgcaccgg agggaagtac ggaacgttgt acggatggtt gcaaaataga agcagagtgt
    21181 ccttactccg cattaagaat ttatcaaagg aacagaagct ggttgcaggt aatggatatg
    21241 ccggatgata aagaaaaaca tgctgctcat atactggaga aattaaaaac caccaattac
    21301 ggccgctgtg tgtaccaaat ggataatgac caacccgatc actacatatg caatattcaa
    21361 tttgaaaata atatcactgc cagtttctct atggaagcgc ttacttccta tgaaggccgc
    21421 agaacaagag tgatgggcag tatgggtgac atagtgggtg acatgaactc atttgtgctt
    21481 actgacttcc gcaccggtaa aaaaacagaa tggaaacaga gtaccgacgg acatggcggt
    21541 ggtgattggc ggttggtagc agactggata caagctattg atcagcaaaa cccttctctt
    21601 ttaacttcta ctatcgatgc ttctatagaa agtcatgtaa tggggtttgc cgcggagaaa
    21661 agccggaaag agaataaggt ggtgactata gagatgtaag tacctattct gtttttcatg
    21721 atcatggttc gaaaggttaa aacagtaaaa ctggattgat ccaaaatgaa actattgtaa
    21781 agaacaaccg tttcatttcc cccacatttc ctatcttcct tctttaattg cttgctatgc
    21841 acaaatcaaa aattgcgggt attggtatgt acgtacccgc caatgtcgtt accaataatg
    21901 acctcctgaa atacatggat accagtgatg aatggataca ggaacgtacc ggtatcaaag
    21961 aacgccacta tgcccatcgt acagaagaaa caacgacaac catgggagtg gaagccgcca
    22021 aaattgctat tgaacgggca gggattaccc cacaggatat tgactttatt gtattcgcca
    22081 cgttaagccc tgattattat tttcccggct gtggggtact gttacaacgg gcattgaaaa
    22141 tgaaagaaat cggcgccctg gatgtgagaa atcaatgctc agggttcctg tacgccttaa
    22201 gtgtagctga ccagtttatc aaaacaggta tgtataaaaa catattggta gtaggtgcag
    22261 aaaaacattc ttttggcctt gacctcagca cccggggaag aaatgtatcc gtgatatttg
    22321 gtgatggcgc cggtgcagtt gttttgcaac caactgatcc atcatcaggt gcagcacaaa
    22381 accagggaat attatctacg cacctgcata gtgatggaga atcagcagaa atactggcca
    22441 tgtataaccc gggcacccat gctaatcact ggaaagaaga aaaatatgcc gactttgatg
    22501 aggctgaaat tgcacagatg ttttttagtc atgccatgat cgataatgga cagatattcc
    22561 cgaatatgga cggacctgcc gttttcaaaa aagctattgt caaatttccc gaagtaatat
    22621 tagaatccct gaaagcaaat agcctgcaaa cagctgacct gcagttgctc attcctcacc
    22681 aggccaatct tcgcatttcc cagtttgtac aacaaaaatt gcagttaaga gatgaccagg
    22741 tgtataataa tattcagcac tatggcaata caactgctgc atctgtacct atcgcccttt
    22801 gtgaggcatg ggaaaaggga ttggttaaag aaggtgatct aatttgcctg gcagcatttg
    22861 gcagtggttt tacctgggcc agcgccttat taagatggta gaatacaacc cctttaagga
    22921 tcgatttgca acttttgaca gcctgttccg gtcattcaat tacaagcctt aaaaatgaaa
    22981 aagtcaatac ttcttttttc cctgatcgta atgtcggtga ctatgcatgc acaacaaacc
    23041 tggactatat cgcaccagaa aaaaacagta ttaaaaaata tcagcgaaga ccccgataat
    23101 aatgttgtta ctattagtaa ggctttgttg aatacaaccg ggtgttttgt tattactttt
    23161 aaagcatatg atacggcctt tacaagaacc gtaatggcct atgataccgt acgcaatggg
    23221 ttgaaaaact gggatgatgt taaaaaaacg atttctatcc ccaatgcaga attaaaatca
    23281 gtgatggccg gaaagaataa aatcaatctc tattactctg agataccaaa agatccggaa
    23341 aaagctgctg ttatccgggt aagattaatt catatctgta ccattatatt gaaataaggg
    23401 ggcacagcaa taaaaacata atagcattga tagtgttatt tttgttatag tgtcccgcaa
    23461 tatcatttat atcatcaatc ctgtttccgg taccagaacc aaaaaggatc tgcagcagct
    23521 aatcgaaaag aaaaccacgg aagccggaat aaaatttcag gttttccctt cagtggccag
    23581 cggcgactac gcatttttac accctattat caaggaacaa caggtaacag atattgtaat
    23641 tgccggaggt gatggcacag tgagccaggc gataagcagc ctgatgaact gtggtgtaaa
    23701 ttttggtatc attccctgtg gatcaggaaa cggattagcg tatgccgcta aaattcccaa
    23761 gcaaccagct aaagcgctgg acgttatttt caatggcaac ccaactgcta tcgatgggtt
    23821 tactatcaat aagcattttg cctgtatgct ggccggcctt ggttttgatg ccaaagtagc
    23881 tcatgatttt gcaaaacaac cgaagagagg attatctact tacgtaaaac aggtaattaa
    23941 aaatttttgg gctgcaaaaa cgtatttatt tgagcttact attgaagaga aaaaatttat
    24001 tattgatgct ttttttatca gcattgccaa tagtaaccag tttggtaatc attttaccat
    24061 cgcccccaaa gccagtttag cggatgggct attggatatt gttatagtaa cgcatcagaa
    24121 taaactgagc ttgctgtttc aaacatttaa acaggtgctt gggaaaaata agctgcaaca
    24181 atcagctgtt tttgaaaaca aaaaaggggt tatttatttt cagacagata aaatagctat
    24241 tgctaatcaa tcagaagctc ctttgcatat tgacggagat ccggcggaaa caccaaccaa
    24301 agtccaggtt gaaataaaga aaaaatgttt ccggcttatt caaccttctg atcactagcc
    24361 gaaacagact ccctgaaatc tctttgtgaa aataactgta atagtttatt aaatccttgc
    24421 cggttgatgt cagaattgat agagctgata atatctttct cttccctgcg ggtcagatga
    24481 aaatttaaag ccgctttgtt ctcctggtta ctggaagcat attgaaaagc tactttgtac
    24541 acaggtgttg cagttgttgt agtgaagtaa tcaaggaggt cattttgaga atactccatc
    24601 atcttaaacc agttgtgctg aagcagcaag aatggtttgg tcgcatgatc tgttatatca
    24661 tcagagagat aaggattttc ccaacccccc gcctgcctgt ccctgatctg gatcactacc
    24721 actccgcttg tattttcctt tatccagtca tcaaaagaag ataagaaacg gagtgtagtt
    24781 tcctggccat aattgtcccg caaaccggca tccatcacat ctattaccgg attggaaggg
    24841 agccatacat tcggcaatac aatcgggaaa gttgcattca tccgtaatgc cgacagcatc
    24901 ctgatattat agggatcctg cttattaaaa aaggaaacaa agtcaacagc atcaggatct
    24961 gctacaggaa tctttgaaga atctgactga ccctgcatca taaatcgtac gggctgtgtg
    25021 gagatgatca tttttcggct atccctagtt atcaccgaat taaaaaacat taatgggatc
    25081 tttgctgatt tttcaacatc tacataatct cccaattgtt tattcagaaa ccctcttgta
    25141 ttctcgttca gcttctgttc aaacgcatag cccctgtctt ttacataccg gtaagggccc
    25201 accgaaaatt tttgtgccgg cgcaatcaaa tccctggcaa aaaatgaagt aaataacggg
    25261 ttcagtaaat cacctgcaat atcatcaatg tattgcttgt tctgcaaccg gatattatcg
    25321 ccctgcaatt tcctgaggta taattccctg aagtaagcgg cgcctatcat tcctcccgat
    25381 gcgccgttga tcaggaatgt ttttttcata atttctccgt tggtgatact gtcaagatgc
    25441 tgcagtaccc ccatagtaaa tgtagcactg cggtgccctc ccccgcttgt gttgatcagc
    25501 accaataaag gcttatcgct ctcctgtttt ttcttccagg cattcagtac cgtgatcata
    25561 ttctgcctgt cagcttccac attctcttta ctgcaaaggt tgagtaatcc ttcttttgta
    25621 taagctggcc tttcattctt attccagtaa tcaagaccat acgctttatt acggggatct
    25681 ataaggtcaa gcttataaaa tacattgagc aatataacaa gacctaccag gtaaggtata
    25741 ctccagcttt gcaggaaata ggtaatagcg ccagccacac ctatcaatat ggaaaagaaa
    25801 atggtaatgc tgcctgccgc tggtaattgg aaaaaagggt aatcaaggaa aaaaccaatc
    25861 aggatcagga acaggaaagc cgcaaacaca gatagaatgg ctgcaaagtg atgccccctg
    25921 aacatagatt caacaaattc ttctgtataa tgtgatacgt ccctggcagg cttcaaccga
    25981 aaaggagtct cgagataaga atttactttt atcaacggct tatcgtgata cacttctttt
    26041 attatgccta aatgggtgat atagtttttc ggctctgcaa ttaccggcat catccgctga
    26101 agaatagaac ggtcggccct gaaaaaatag ataagcgatg cggccagtat gaaaataaac
    26161 ccgcttgtaa aacccccgaa aaagaaaagt atttcagttc tgggtaccaa ctccttaaaa
    26221 tgaacaaaat gataggcctt gaaaaaataa aagaccagaa acaacccggg aatgattgaa
    26281 ttattgatgc aaaattttaa gaacggatta gtagctgccg ctaaaaaacg aaaatgccgg
    26341 ctgaaaagaa taaaggaggt gatgttccag ctcatgataa acatacctac agaagctcct
    26401 acaattgcat agctgataga gtttacattg cccaggtact ctggtgccag gaataaagaa
    26461 tccgctccga aagttttcat aaaagtaccg tttacggtac tgaacagaat gacccagaac
    26521 aataataaca cctggtactt cctgaaatgc aggaataata attgtatagg gaaagaataa
    26581 taaaatcctt ttagccaggc tttcatgaac gtggaaatgg ttgtaactta aaaatactga
    26641 ttattgactg gtaagtcaaa taagcaaacg aaggaaaatg ttaataagca gctttgttgc
    26701 gtacaatgaa caatatccat acaagggata ataacagtaa catagcgaca aactgatgta
    26761 attgtgccat ccattcaaaa acaccccatt gattagggat tatttttata ctggtcagca
    26821 cagagaatat gccaagtact acttgtataa acactaataa aagcggccac cagcgggttt
    26881 tattataaag tgaagagccc ggggttttat acagtttcag cgtaagaaat agtaccagcc
    26941 ccaacaggat ataagccaat ccacgatgga taaaatgaat agtgatctta ttatcgacca
    27001 ggttcagtaa tccatgctca ttacccatca taccgggggg aaaaagatag ccattgatat
    27061 ccggccaggt tggcgctgca gttgctgctt tatgacccgc cataaaagct ccataaacca
    27121 gttgtactac cagtactgcc attattaaca aggtgagatt tctgattgac ctgctaataa
    27181 caaatttttt atccggcacc aacaattcca tggcaaacca aaaagtataa ctcagcaagc
    27241 ccagggcaaa aataaaatgc aaagccagtt ttgttggttt tacatagata gcatccccgg
    27301 ttaatccgct tgccaccatt atccatccga tagcaccctg taaagccccc agtaaaaaaa
    27361 ggatgatcat aggcttcacc atcgccggct tgaagcgccg ctggattaaa aagataataa
    27421 aaggaacagc gaatacaact ccgatcaacc gggcccagaa acgatgaaac cattcccaga
    27481 aaaaaatgaa tttgaaatct ttgagtgtaa aatcaaaatt gagcaggttg aattgcggcg
    27541 tttgcctgta tttttcaaac tctgcgaacc actgctgttc actcattggt ggcaaggttc
    27601 ccgtaaccac attccattcg gtaatagaaa gccccgaacc tgtgagccgg gtaataccac
    27661 ccagcaaaat ctgaataatt atcattccaa caccaatcag cagccatgtg gctactggct
    27721 ttgaagaacg atctgttagc tcatttgtca ttgcggatgc aaagttaaca tccctgtttt
    27781 ctaaacaata tttttgttgt acaaaagacc tctactatca cgggccggat gaacattgta
    27841 atatgaatat ttcgccatca tggggtttgt caatcttaat caacaatcat taattttgag
    27901 aatgaggttt ttatgtatac tattattggt ttcaaatgcc tgccttgccc agttcgaaac
    27961 aaaatgggta aaatccctaa aaatggatag catcaatact gacgacgaaa tattttattc
    28021 attctttggc gataaagtat acttgttgaa tgttcatgcc ttttacaagt ccgaaataac
    28081 aacaaaccct gatggaagta ttacaatgag tatggggata aaaaaatcaa acgattcctt
    28141 acagaaagcc agtatttata aattggaaga tgatggcaca ctgcaattaa aaaaccagtt
    28201 tgaattcagt ccaaagtttc agataaaaca atttattgtt acagaaacag acagctatct
    28261 ggtttatggg gctgaaatgt attttttagg taaaaaaaca tcttttatca aagaatggca
    28321 aggcaaaaca gggttttcag aattttcttt cagtgccgaa agcagttcta gtataaaaaa
    28381 tatttttaga gacagaaact ttaccagtgt tatagcatgt caattcagcc aattttcaat
    28441 gcgtccggcc ttttatactg taaattctaa agagaaaaca ctatttgtta aaactccgga
    28501 tagtgtacac ctgaattatg attttatgag tgatttttct gaggggaaat attatatcgc
    28561 tggaaattct cggataaata ctgatagtac agacaagagc cccttttact caagattact
    28621 tttatttgac aaaaatggaa gtttacgaaa agaagaaatt tatcaattag acgattcatt
    28681 gcaccatttt gacgttcggt ccctgtccgt acagaacgaa gaggtagcta ttacaggcct
    28741 tctttactac agagattaca agaaccttga gagaaggtgg atagtacagg ctgtgaaaaa
    28801 aggaaaactt ttgtggtcag ataccctgcc ggaaggttat atgccacaat cggttcacca
    28861 tacaggtaat agtattattg tagtatcgct ttttaccaat aaaccaggaa agggctataa
    28921 tagctatcat ttccttagat ataataagca cggggagcta ctagaaaatt tttcgttgga
    28981 taatataaag cctgaatgga aaaacatagt ggtggttcat gtttccgaaa aggaattagt
    29041 ttttgcaggg aagaacattt tcaactctct ggttattgaa aagatatctt tttaaaagat
    29101 gcttgcccag tattttcaaa cagcactaac agaagccggc cttgacgaag ccggccgcgg
    29161 ctgttttgcc ggacctgtat tcgcagcagc tgttattttg cccaaagatt tttatcatcc
    29221 attactgaac gattccaaac aattatcaga aaaactccgg aatcaactgc gtcctgttat
    29281 tgaaagagaa gcggtctctt ttgctgtagc cagcattgat aatgaggaaa tagaccagat
    29341 caatatcctg aaagcatcct ttaaagctat gcacctggca ttggataatt taaaaaagag
    29401 accgcaactg ctattgattg atggcaaccg ctttattgcc tataagaaaa ttccgcacca
    29461 gtgttttgta aaaggtgata gccgttttgc ttccattgcg gcagccagca tcctcgccaa
    29521 aacgtacaga gatgaatgga tgcaacaact gcatagagag tttccacact atagctggga
    29581 ccggaataaa ggatatggta cagctactca tcgaaaagca attgaagaat ttggcttgtg
    29641 caaataccac cggaagagct tccgattatt tcctgttcaa actgaactat ttgaaactgg
    29701 tcaaaacgat acctataaat catcttaatt ctcccattca cctaatccct gccgtatcag
    29761 tatgggttca ctcccggtaa aatccacaat tgttgaggga atcgttcccc cgatacctcc
    29821 atcgaccaca taatccactt cattcctgaa attctcatac atcacttccg gatcagtata
    29881 atcttccacc atttctccgg gcagcgaagc gctgagaatg ggacggccga gttcattaat
    29941 aatggccatc gcaatattgt tatcaggaat ccgtaaccca atagtatctt ttttgctttg
    30001 cagaattttg ggcaccattt tactggctgg caaaataaaa gtgtacgggc cgggcaagtg
    30061 gtccttcagc aaccggtata cagggttaga tataggcttt gcatattcac tgagatgtga
    30121 taaatcactg caaacaaatg acagttgtgc tttacggggc tcaacttttt ttatacggca
    30181 aattctttca accgcttttt gttgtaaaat atcgcatcct aacccataaa cggtgtcggt
    30241 cggatatata atcacacccc cttgttgcag gctttccacg atttgcttta tcaatcgtgg
    30301 tggtgggttt ttgggatgaa tttgaagtaa catgagcaaa cgttcttttt aaaagtacat
    30361 ggttttgcca gaaatcccgc cttcgttggt atttgactta caatttgtaa tttggtcact
    30421 actaaaaaac tgctgactat gcaattaaaa ccagttgccg tttttgattc cattgatccg
    30481 gaagtgttca aaaaagaata ctatgaaccc tgtctacctg tcgttataaa aaacctggct
    30541 aaagactggc cggcttactc caaatggaac tgggattatt tcaagcaact cgttggccag
    30601 aaaaaagtgc ccttatacaa taatgtgaaa agcgatgcgt atactcctat caacacagct
    30661 gacgattaca aaacatttgg cgaatatata gacatgataa gcagcggccc tgctgcatgg
    30721 cggattttcc tgtttaacat tttcgaccat gccccgcagt taatcaatga ctttacctgg
    30781 cctgaacacc tgatgaaagg ctttgtaaaa aaatacccga tgttatttac cggtggtgcc
    30841 agcagcataa cccatatgca ttttgatatt gacatgtcgc atatcctgca tacacaattc
    30901 tgcggacgga aaagggtact catgtttccc taccaggaac aatataaact ctaccgaaaa
    30961 ccatgggaag ttcttagcct cgctgatttt tccaactatt atatagatgg caaagtagat
    31021 tatgaaaaat tccctgcact taaactggcc gaaggtcatg attttattct ggaaccggga
    31081 gataccttat ttatgcccgc aggatactgg catcatatgg aatatcttga tagtggcttt
    31141 gccatgagcc tgagagcact gcaaccctcc gttaccggaa aactaagggg tgcctggaat
    31201 ttatttggta tgcgcagcat tgataccgtg atgaaaaaaa cggttcctaa atggtggtat
    31261 gaaagaaaag agaaaagagt ttttgagaat gcggcgagag tgctcgccag caattgattc
    31321 gcaatagaat ggatgctgta aaccttagtt taacttttct tcaattcatc aatcgtttta
    31381 cgcatccagt aatctgtaac ataatagtag ctgacagtgt gaaaagccac ttttccttct
    31441 ttatctacaa ttacctgcgt gggataaccg gttattttgt atgaatcggc tattttcttt
    31501 ccggccggta taacattata tttaaactca ttattcttaa gataaggctc cagttgttct
    31561 ttctgatcta atgcaatagc aataaaaatt atattcgaat cggacttata atcatcaact
    31621 attttattaa gatatggacg ttcctgcttg cagggcgggc aggtggtgaa ccaaaaatta
    31681 attaccagca cttttccttt taattgtctt gtatccacca ggttaccttt catatcatag
    31741 gcgctgaaag gatcaaattt ttttcctgtc ttaaaagcgg tagtttctgc aggctttgga
    31801 gcagccatgg ctcttttcaa ttgctcctct ctgctgatac ctgataataa aaatcctttg
    31861 cttgcatctt tgggattggc aggtagtatt tcatactctc cacccatcat tacaagcatc
    31921 gcttcattgt aattgtattg cttgcctgtt gaatccgtaa atacacttgt cttcaacacc
    31981 ctgtactccc ggccattata ggtaaaggag aactggctat tggctacaga tactgaaaga
    32041 ataaaaaaaa tagaaataag caactgcttc atatagaaat cccaggatgt taattcaatg
    32101 aagtaaatct tttgatttac caatcaacta aaatgcctta tcaatcagga gcattaattc
    32161 acgaaatcgt ccacacctca ttccccttta acagtttgtc gagatcggcg ggctttcttg
    32221 atttagcctc ttctaattgc gctgccattg catcttcata acaagttctt tccgttttat
    32281 aaaaaacacc aaacggtctt ggcaggtggc cttcattgtg gggatcatca aaaatgcggg
    32341 ttaatatttg agctttatat atatcatttt catcatgtac ccaaagctca tccacactgt
    32401 ggccttcatt gagattaacc actaccggcc tgaaaccatc cagtttgatc cccttatcat
    32461 tggctgcacc aaatactaac ggcttaccct gctccaggaa taatgtttct gccggtttgg
    32521 atcccttttc tgtaaatatt tcgaatgcac catcattaaa gatgttgcag ttctggtata
    32581 tctccaggaa tgaagctccc ttatgtccat ttgccctcag caacatagct tgtaaatgct
    32641 tggggtcacg gtccattgtc ctggctacaa atgtcgcatc agcgccaagg gcaagggcgg
    32701 ccgggttaaa aggatgatca atacttccca ttggggtact cttggtaatt ttatgttctt
    32761 ccgacgtagg ggaatattgc ccctttgtca aaccatatat ctggttattg aataataaaa
    32821 tattgacatc aaaatttctg cgcagtatat gtatagtatg attaccgccg atactaaggc
    32881 tgtcgccatc accggtaacg atccatacac tcaattccgg ccttgaagcc ttcaaacctg
    32941 aagctatggc cgcagcacgg ccatggatag aatgcatacc ataggtgttc atataatagg
    33001 gaaaacggct gctgcaaccg atacctgata taaaaactat attttcttta gggattccca
    33061 gggtgggcat cactttctgc acctgtgcca atatagaata gtcaccgcat ccgggacacc
    33121 agcgtacttc ctggtcggta gcaaaatctt tagcagtcag ggcaggggca attgttactt
    33181 cactcatatt aaataggttc cggcaaattt aatgaatatc agtatcggat accgatcaga
    33241 aaacaatgat tttcgtaccc cgggcggcta acttttatca tttataagct cttcccagta
    33301 ttccgcagcc cgacgggtat ggggaattac gatggtacca cccacaatat tggctacagc
    33361 gaacacttca tacatctctt cagttgtcac ccggagttca tggctttttc ccaggtgata
    33421 tttgatgcag tcatcacaac gaagcaccat actggctacc aggcccagca attctttggt
    33481 ttttacatcc agggcccctt cggcataggt atttgtatcg agattccata gacgtttgat
    33541 caccaaattg tccttgctca aaataatctc attcattttg gtacggtaag cattgaagtc
    33601 ttctacaaga ttgctcatga cataagtttt tagtcatcaa atgtatagaa gttagccttt
    33661 tatcatttac cattgccgga aacgataatc aaatcgtatt ttcagcaacc aaaatttttg
    33721 aacatgagaa aaattattct cgtccttttt attttacaat cagctatttt ctctattgcc
    33781 caaaccggaa aagaaccggt taaggtaact gacctgttga aaataaaatc aataagcgga
    33841 gtgaacctga gtaaagatgg cggtaaagca atttttactg ttacttctat tgaaccagac
    33901 agcgataaca aactggaata taaatatata acacaggttt ggatggttaa tacagatgga
    33961 gcatcctcac caaaacagct tacaacccat aaagaaaatt cttcccaggc agtattgagc
    34021 ccggatggta aacaaattgc ttttgtcagg gcagttgata ataaacaaca ggttttttta
    34081 ttgtcattag aaggtggtga agcctctcaa cttactaaac acaaatacgg ggcaggcaat
    34141 cctaaatgga gcccggatgg aaagcagatt ttgttctcgg caagtattcc actgaaggat
    34201 ttattaaaag attctaccct caaccctgat aaagaacttc ccaaatggcc gatggagaaa
    34261 cccggtatag ataataaact cacattaaaa gcaactacct caaaagcaga ccctgatggt
    34321 actattgaag aaataagagc ttacctggaa aataatgcaa ttgacaaaaa agccaacgta
    34381 atcaacaaat tgaatttcct cgatgaaatg aatatcaatg ctgaaatgag ctttaatcac
    34441 tatttcctta ttgatcttag gcctgacaca aagcctgttc ctgttaccag ggggttttat
    34501 cgctataact ctgcagattt ctcgccggat ggcaaacaat tgatccttgc aggtaatatg
    34561 gattcattac aacatcctga ccgggcactg gaaaatgaaa tatacatggt gaatactgac
    34621 ggcaccaaat tcagaaaagt agtaggagaa aaagacaaga atttcaattc gccaagaata
    34681 tcaccatccg gcaaatggct gacttttcaa tatggcaata cctcatttgt cagtgttccc
    34741 caactggcaa ttatgcccgt caatggttct gtaaaggata aaattgatat cccgttcgac
    34801 agaagtaaag ggaatatcac ctggagcaat gatgaaaagt atatttactt cacagctcaa
    34861 agtaacgggg gacaacccat atacagggtt gatttaagta gtggatggac gatatatcct
    34921 ccgttaagta cacttaagga taaagacaag aatataagag tagatcagct tactgattat
    34981 aatgacggaa ttggaagttt tgatatagca ggaaataaat tagtgtttgt taaaacagaa
    35041 gtggccaatc cttttgaaat atatgtggca gatgctacag ccaaaaatca aaaacgaatc
    35101 agcaatttca atacagaatg gattgcaaat aaaaagctga gcttccctga aaaacatagt
    35161 ttcaaaaatg aaaagggaat ggagatcgaa tactgggtga tgaaacccgc taactatgaa
    35221 cccggcaaaa agtatccgtt gttattagaa attcatggag gtccatcagc catgtggggg
    35281 ccgggtgaaa gcagcatgtg gcatgagtat caatatttct gcagtaaagg ttatggagta
    35341 gtgtattgca atccccgtgg aagtggtggt tatggtttgg attttttaag aggcaatatc
    35401 aatgattggg gaacaggccc aaccagtgat gtgttagcca gtttggataa aactgttgcc
    35461 gaaggatggg cagacacttc taaattattg gtaaccggag ggtcttatgc cggctacctg
    35521 gttgcctgga tcattgcaca tgatcagcgt tttgcggccg cttgctcaca acgtggagtg
    35581 tatgacctgg ccaccttttt tggagaagga aatgcatgga gactggttcc caactatttt
    35641 ggcggttacc catgggatgc tgccgctaag gcaacactgg aaagagaatc tccgattaat
    35701 catgtacaga atataaaaac cccctacatt atatttcacg gggacaatga caggcgtact
    35761 ggttttgtgc aaagtgaaat gttgtataaa agtttaaaag tgttgggcag acctgtagaa
    35821 tatgtccgcc atcctgccgc aacccatgaa ctgacaagaa gtggtaacaa ccggcaacga
    35881 attgaccaga tgctaagggc ttatgaattc tttgaacgct ggatcaaaaa ataatttttg
    35941 acagatacca actatgcact tgtaaaatcg tctaaatcgg caatatatgt acagtaataa
    36001 aataaaagta attacgggcc tttcaatatt cgttctgctg ggagtagcag cagtacaggc
    36061 tcctcatcaa aaagaaaaga accttaaaat actgcccaaa gacatcagcg atgaaaagct
    36121 tgacagtata atgcagactt ataataaagc actgggtgta aaatgcgatt tctgtcacgt
    36181 taaacaaaaa gtgtttcccg gagattttga ttatgcttct gatgctgaac cgatgaaaga
    36241 aaatgcaaga gaaatgatgc ggatgacaat tgagatcaac aaatcaaact tctatttcga
    36301 taaaaacata agacctgaat acctgaatac agtgacctgt aaaacctgtc atcgcggcga
    36361 accttttcct ccggaagatt aatcactatt tacaggattt caaccattct tttataccag
    36421 ctataatctg tgctgcaaca gctttatgaa attccgggtc aagaattaat tgttcatctt
    36481 ctttattgct caggaaagcc acttctacca ggcaatttgg atattcagta ggtccgctca
    36541 atgcaaaatt gaagctacca atattgccaa attcttttaa ccctaattcc agcaggtcct
    36601 ttaaaatata ctgggataac ggccggaaac ctatatgccg gtaataagta ctggttccct
    36661 ttatactatc cctggctgaa gaatttagat gtatactgat aagaaaatga gggtcctctt
    36721 tttgtaacgt caatatcctg tccggcatat tcaacgtagt atccttatct cttgtcataa
    36781 acacatctgc gttttcatcg agcaacaatt tttccagttg ctttgcaaaa agcagcgtat
    36841 actctttttc cagaattttt gtagttatac ccctagcccc attattatct cccccgtgtc
    36901 cggcatcaac tgccactttg aggtttttca gatctggttc tgccggttgc cttttcactc
    36961 ttatcaccag tctcttttcc tggtaataaa tgctatatcc ccagtgctgt ttgttcttta
    37021 actctataat taccctgaat acatcatctt ccaattgttc gtgataaaca tttttaattt
    37081 cctctgcagt cgaaagttgg gttatccagt ttgtattgcc ggtagctcca aagatgtcca
    37141 caactatttt tgaaggattg atctgttgca tgctcctgta aggtaatttc tcatcgaggg
    37201 tcacggttac ataatcatac ctgtcatcac cagacactct ccagctattg gtcaggtaat
    37261 agggcaatag tttaatatca ttttctcttt gaaaattttg ttttggtaag taggcaaaat
    37321 ggtcttttga gagttggact ttgtaattga ctatggtact gtcaactact ttaatagtga
    37381 ccccggtgtc caggtaagtc atttttgcac caccaagacg gtctattccc agtccatatt
    37441 caagaaatgg taaaatcccg gttgtttttc cggcgataaa attgttttgt atggtttgtg
    37501 cattggttga aaaagcccag aaaacacaaa caaaacggag gaatcgtatc ataccagaaa
    37561 gatagcataa aaacactctt aacagctgtt ccgtattaat acggaccgct atcacagttt
    37621 tactgaattt tggcacaaag agttctatgt aagcactata tgtatctgta tcccgttaat
    37681 attgcagacg gcaccgcaac caggcggttt acgttttgaa cctgtatcct tagaaaaaac
    37741 caatagacta tgacctggaa ttccataatg ggctttgttt cctcgtttgc cctttttgta
    37801 ccgattcttc taatattgat ccttcgcctg gggttgtaca ggagttttcc ttttttactt
    37861 gtttattact gctccgttct tgtttataat tttttgacag aaggctacat taaggcaagt
    37921 gatgatatta ttcaggtttg ggggcttacc aataatctcc tggatactcc gctgatatta
    37981 ttttttctga tctatttcag cacctcaaaa agctttaccc aaaaaatgaa gttgctggct
    38041 tttacattta ttttatttga agcaatagtg cttacaacca ttggatttaa tatcgatgca
    38101 ataaccatca ttatggggcc cggtttgctc attatttgtg gattatgcct gtatttcttt
    38161 atccggcagg caaagctggc tgttgaaaac cgcaaagcaa ccggtaaggc tgtcattatc
    38221 acttcattgc tttttgggta tggctgctat ggcattattt atctcatgta ttatattttc
    38281 aaagcacatt ttcgtgctga tggcaccgta aatcagcagt acgttgcaga tacatttctc
    38341 gtcttcttca ttgccaccac tctttcatca atcatcatgt gtatagggct tgtcattgaa
    38401 agaaaaagaa ttcaaaaact acaggaactg aaagtaaccc gtaaagaact ctccgcaata
    38461 tacacagaca caaaaagggc tgccccttta agaacagcca tgcttgactt tgacagagat
    38521 cagtggaatt aaaaacagta ccttccttct gttatcattt tatcagcaat ccttcttctt
    38581 gcttctttac tattgaaggg ttctgccttt gtaaatcgct tcagccccat taatatcatt
    38641 ctttgctcat caccatcagc gaaagagttt accgcatcct tacctgattt attcactttg
    38701 tctgcagcat cataaagata ggttctcatc atatccaggt aatcctggca agcggcttcc
    38761 cctttctgat ctgccatctt cattacccta agcagtacac tttctgcatt aaatgtttct
    38821 atcgccatat ccgctatatt catcaaaact tcctgctcat tctcgatttt catcattagc
    38881 ttttgaacag ccgcaccgga tgtcatcagg attgcttttt taagatttgc aaccagtttt
    38941 ctctctttcg caaacggggc atcatcttct gcaccaaaat caggaatgct cattagttcc
    39001 ttttggacag ccattgcagg tcccatcaga tccaaacgtc ctcccattgc tctctttaat
    39061 gtcatatcaa ggatcagtaa ccggttaatt tcattggtgc cttcgtagat acgattgata
    39121 cggctgtcac gataagctct tgaaatatta tattctgcac taaatccatt tccaccatgc
    39181 acctgcacac cttcatccac tacataatcg agtacttcgc ttccatatac ttttaatata
    39241 gcacattcaa ttgcatactc ttctgcacct cctaacaaag cctcactgaa aggtttgcct
    39301 tcagccagta attggtgttc tttatcatca atccatttgg cagtccggta caaggctgtc
    39361 tcggcaacaa aaactttaat ggccatttca gccagtttgt gcttgatagc cccaaaattt
    39421 gaaataggtt gtttgaattg ttctcttgtt ttggcatatt ccacggtacc gcttaaagat
    39481 tttctggcac ctcccaaagc tgctgcacat aatttcagcc gtcctatatt gagaatattg
    39541 aaagcaatct tgtgtccttt cccaatctct ccaagcaagt tttccaccgg aactttacaa
    39601 tcctggaaat ataactgaac tgtagaagaa cctttaatgc ccatcttatt ctcttcaggg
    39661 ccctgtgtaa aaccctccgt ccctctatcc acaataaaac cggtaaactt gtcgccgtcc
    39721 acttttgcga acaccgtgta cacctgtgca aaaccaccat tggtgatcca gcacttctgc
    39781 ccgttcagta tataatactt accatcatca ctcagttttg ctgttgtctt tgctcctaaa
    39841 gcgtcactac cactgtttgg ttctgtcagg ccgtaagctc cggcccattc gccggtaatt
    39901 agtttgggga tgtacttttc tttttgtgca tccgttccaa aatataaaat tggcagcgta
    39961 ccaattcccg tatgtgccgc aattgctaca gaaaaagaat ggcctgcgcc gagatactca
    40021 ttgatgatag tggaagtaat aaaatctttt cctaatccac catattgctc cggaaatgaa
    40081 gttgctaata atccctgttc cccggccttt gttaccaatg atttcatcag tcccggctca
    40141 agactgtcaa tacggtcaag gataggataa atttcagctt ccaggaattg attacacata
    40201 tcccggatca taaactgttc ttcgttgaaa ttttcagggg taaaactttc aaaaggcaac
    40261 gattctttta ccagccattc tgcacccttc agtacagttt ttgttgctgt tacagtactc
    40321 attgttgtaa ggtttaaaat ttaatatttt aatttctaaa aaaaatgccg gattcttata
    40381 acgatccgtt attaccaatt aattatttca aattatcata caccacctgc aatacctcct
    40441 tgcatatatc caccttatca gcaggtacag ttattaaagc ttcgttcagc gtctcatcag
    40501 ccagtgccat tgtctcttcc tgtaattttt gtgcctgctt ggtcagatat attaaattaa
    40561 tacgtctgtc actttcagat gcaacccgtt taacgagatt caacttttcg agattatcaa
    40621 ctaaacgggt aatacttggc ttatcacgga aagtagcgtt acacaattcc tgctgactga
    40681 taccttcctg tttccacaaa tgatacagca cactccattg ttcaatagta atattcaacc
    40741 cggctgcatt aaacttcttt tgcaacctgc gggcaatggc ggttgaagct ttaccagtga
    40801 taaaactgta gagttcccct ttcttaaatt ggttgtttgg catatagttg tttgtgcaac
    40861 tatccaaaat taatattata tttgaaatag ccaatttaat ttttcataaa atttaatccc
    40921 cattcctttt tggaactaaa gcatcttata tatagaaatt ctaccattgg gtactattgt
    40981 ttcggctcag ggctgaaaca agcggtttgc tttcatggat atggtgagga tgcttctatc
    41041 tttcatttcc tatccaacta tgctggaaat caatatactt tctattcaat agaccttcct
    41101 ttccatggca gaaccgaatg gaatgagggg ttgaatttta cccggcagga cctggtactg
    41161 attattgaaa agataactga aaaagctgca aaagagctga cccttattgg ttttagcctg
    41221 ggcgggagga tcagccttag cctgtttgag gccatcccag agaaaattga aaaaatagta
    41281 ttactggccc cagatggatt aaaagtcaat ttttggtact ggctggccac tcaaacctgg
    41341 ttagggaaca ggctcttcgc tttaactatg aaaaaaccag gctggttctt tggtctcctg
    41401 aaaatgatga acaatgtaaa actggtcaat gccagcatat ttaaatttgt taactactat
    41461 atcggcgatg ctgaagtgcg ccggttatta tactcccgct ggacatcttt aagaaaaatt
    41521 aaacctgacc tgcccgtcat taaaaactta atccgggaaa ataaaacaac agtacggttg
    41581 atatatggaa aacatgaccg gattatcctt tcatccacag gagaacaatt caaaaaagga
    41641 attgaagaat attgcactct cactgttatt cactccggtc accaggtgtt gcatgagaaa
    41701 catattaaag aaattctacc ggctctgatg caataattag ctttgtgata ttggtatgat
    41761 cattttatac accacgatac tattatttct tctttatagc ctgcttatca tttattactg
    41821 gcaagcctgg aaatcgattc cggattattc gatgtcattc actttacaac aaaccaaaat
    41881 aagtataata atcgcagcaa gaaatgaaga agaaaatatc ggcactttat tagcagctct
    41941 tcaacgacaa acttatccgg cagcattcac ggaaataatt gtagtggatg atcactccac
    42001 tgattctaca gtagcaattg cccgtcagtt tccccggata aaaatcatac agctaaaaga
    42061 agataatctc aattcttaca agaagaaagc catagaaacc ggcgtagcct ccgcaaccgg
    42121 ggacctgatc gtaactacag acgctgattg tttaccgtca cctgactggt taacaacaat
    42181 agctgcattc aggcaagaaa aacaatctgt atttattgct gcaccggttg tgtttactaa
    42241 tgacaactcg ttactgcaaa tttttcaatc catagatttt ttagtattac agggtattac
    42301 gggtgcctct gtttttaaaa atagccactc catgtgcaat ggagcaaatc ttgcctatga
    42361 gaaaacagct tttgataaag taaatggctt tacaggtatt gataatattg catccggcga
    42421 tgatatgctg ctgatgcata aaatctggaa gcattacccg gacagggttc attatctcaa
    42481 atcaaaagca gctattgtca ccacccaacc catgaaaaca tggaaaactt tttttaacca
    42541 gcgcatccgt tgggccagta aagcaaagaa ttacgatgac aaaaggatca ttgctgtatt
    42601 gttagtggtt tttcttttca atctttcttt cctggtattg gcagttgttg gtttctcgtg
    42661 ttatcaatat tggctttacc tcgccggatt gtggatttta aaaacaataa tagagctgcc
    42721 atttgtgaga agcgttgctt cattttttaa caaacgggca ttactcaact atttcttttt
    42781 ctttcaacca cttcatatac tatataccat tgtttccggt ctattcgggc agataggaaa
    42841 gtatgaatgg aaaggaagaa gggtaaactg atatcaatag aataaaaagg cataccgccg
    42901 ggaatattct cctttacgcc ttttctcttt caacctctta gcttaacttc ccggcatgag
    42961 tcaatcccaa tcattccagg ccgcagcatg gcgaaggctg aagaagaaca aaggcgcagt
    43021 ctttgggttg attgtgatta tactggccgt actggttgct gtcttcgcat atttcatcgc
    43081 cccggaccca tcaccgtatg caaaccgcat cattcttgaa attggcggag agaaaccagg
    43141 ttacaaacag ccttttatta aaataaaaaa agataaagat gttgtcacta ctagcttcgt
    43201 tacacaatta gtttctggaa aagaagatgt ttattactat gttccgattg tgaattatga
    43261 acaaaaagag gacagtatta tcgtgcagaa atatattgat gaaggcgtta atgaaagatt
    43321 agcctatcac aattcccaaa cagctgcctc tcctgttgtc acaaataaat ttctggccgg
    43381 tactgataaa tacggtagag atattctgag ccgtattatc gttggtacaa gggtaagtct
    43441 gagtgtagga ctaatcactg ttatcatttc actgagcatc ggtttgattc tcggttcact
    43501 ggccggtttt taccggggta aaatggatga tgtgatcatg tggttcatca atgttatctg
    43561 gagtataccc acactgttat tggtatttgc cattacttta gcattgggaa aaggtttctg
    43621 gcaggtattt attgcggttg gattaacgat gtgggtaaac gtggcaagat tagtaagagg
    43681 acaggtactt ggcgtaagag aactggaata cgttgaggca acaagagctc ttggcttttc
    43741 caacttcaga acaatagtac ggcatatatg gcccaacatc atggggccag taatggtaat
    43801 agcagcctcc aactttgcct ccgccattgt tattgaagca ggattaagtt tcctgggtgt
    43861 aggtgtgcaa cctcctatgc ccagctgggg attgatgata aaagagaact acaatttcat
    43921 catcacacag aatccaatgc tggccttagc accgggtttt gccattatgt tgctggtgct
    43981 ggccttcaat ctattgggaa atggattaag agatgcactg aacgtccgtg gaaggatctg
    44041 acctttcatc ggtttgcact atagatgtac ctgatcctgc atttatatct ataataatta
    44101 atagagaaag acataaaaag aaaaggctac caattttatc cgtctcgatc agatcgctta
    44161 agaaattcac aacaataagc atggtaagta ttatgccgat agtaattgca gttgtcctgt
    44221 aaaatatatc ctgtacccgg tgatataatt tttgggcata atacagcata ccacccaaaa
    44281 gaataataaa gagaagcaat cccggaactc cctgttcaat gactgtcagt aaaaagtaat
    44341 tgtgaacggt agaatgttcc ttgttattgc tgacccatgt tttaaaagcc ggaactgcat
    44401 agggcttata attatcataa aaggtattcg gaccatagcc ggtcagccaa ctatctttta
    44461 tcatcctgac accggcgatc cagcgataaa acctttcggc agttgaaaca tctctgagtt
    44521 tataagtagc tataaggtgt tccttaaatt ctttatgaaa gattgttgtc tggtaatcat
    44581 gagcatagtg caggtagcgg tcattacttt ttaaccaaaa cactgcagct atagttacaa
    44641 taatgaatga tatataggcg accaccaata actttttctt tatgagccag tacgaaagaa
    44701 taccaatcat gagtgccagc caggcacccc gggcatatga aaaaaataaa gcaaccaacg
    44761 caataacaat aacaactcca agcacccact ttaagtttcc ccgctctgcc aacttccggc
    44821 acgcaaataa taccggcacc atacatacca gcatggcaga ataattaaca tgattacgaa
    44881 aaaaaggagc aacagcgtca tttatagttg caaaacttaa tccgctattg gcatgcctta
    44941 taagagcagt cgctgcaatg aataacattg aaccagcaac caactgtgct gcaattttta
    45001 ttgatttttt agtactgaaa accagcaggg gcgccagcac aaaagcactg atataccagg
    45061 ttttcgccag aagaaatttt gttgaaacaa gcaaattcgt agagaataat actgctatca
    45121 tctgccacac aaatgctgca tacaataaca atagcaatgg atgacagcgc attttcttcg
    45181 aaacaactcc tggcgaaaat agccagtatg caaggaataa acaggaagtt agcaacatta
    45241 agaactcatc gggtatatct gtgccgagtg ttgaagaaaa attatactca aaagaaagcg
    45301 gtaacgtcaa taataacaga aagaaaatag tattccgatt ctcccatccg gcataaaaaa
    45361 gtatagtggc aaaaggaata acaataaata aatattgatc caaaaatgct gatacagcaa
    45421 tggcaagtac aaagaaccct gcaaaaaagg caaacaaagc cttgcttttt ttagcaacag
    45481 tattcactgg cgggtatttt ttcttcgctc cagaatcagg gccagaaata acccgaataa
    45541 aaagcttagc actgctgtag caattaggat ctgcattcgt ttaggcctgt caggccagtc
    45601 tgatcctttt gctctttcca cgggtatgat caccggcggt ttgctatcaa ccatcagctg
    45661 gaattcccca atcagtttct cgtattccat taacttattc tgcagtacca ttttgttctc
    45721 cggtatagta cctgaaatac tatccagctg ttgctgtatt ttttctttcc cggacaataa
    45781 ccccctgagc gtcgcttcat tacccgcact ttgaaggtgc tggtgaattg aatgcaattg
    45841 atccatgata gcattagcta attgagggga aagattttta tccgtatccc atactttcac
    45901 tttcagctcc ccatattcac tcttcataac tttggtattc ttcttaagta ttgaagcggc
    45961 tttattaata gctgcatccc ctttttcatt catcttataa tggtcataaa ggttatactg
    46021 ctgtgcaacc gtcaaataaa cggtatcgag attacctgta ccaacaatca aatccagatc
    46081 gtcaggagta cctagtgtgg aataaagttg ctgaacattt tcattaaata ttttcgatct
    46141 gtccgatgag taagagctgg ccggaacagc agtaacaaca gatacatatt gaagtggctt
    46201 tgaaaataca ataatactca ctgcaagtaa tgacacaatc actacagcaa gtatttgctt
    46261 ccaccatttt tttatcagtt cggtaatatc aggcattgaa agttattttt ttaattatta
    46321 gttgtttgtt ttaagaatgt cttccattta tgaatgtctg acagttttaa aatgatggaa
    46381 gcagtaatac tgattacaaa taatccagcc aatagccacc acttatttaa aatatattta
    46441 ttcccggaat aaaggaaccc gaacatcagc acacccataa aaatatacat taacagggac
    46501 cggtagtgaa tattaatacc tagtttttgc cgggtatata acatagtgcc aatgccacaa
    46561 aatccctggc ttaccaatgc agcaaaacag cttcccctgg caccaaggtc tggtatgagc
    46621 agaagattta aaagaacatt cgtaacaacc gatgccagta ctatatagca aaagggaact
    46681 atatgacctg tagcggtcaa tacagttcca taaacctgaa ctaacgaata agcaattaat
    46741 actggcaggc accattgcaa gacttcaatt gcttcagcat cctggtcatg atacaaaatt
    46801 tcctgtagcc agggagaaag tatagttgct ataactgcta tcattaagga aaataccagg
    46861 agcaggtggc ggctgttcag tatagcttca ctcatgtcct ttttaaggct atattgcctg
    46921 gcgataaatg gcaacagaaa tgaagctgtt aaatacccag gcatatttgc cgcatccaat
    46981 aaacggtaag cccccgcata aatacctgtt tcatacgcac cattgccgtg cagtctttcc
    47041 agtaaaaatc catcaagccg gtaatgagca gacataagta agacaattag tccgaaagga
    47101 agagctgatt tgaagatccg tttattgaaa agtggagatg acggtaatga aaatataaat
    47161 ccctttctca acaaaacaac tattgaaatc agcaatacaa tggcagagca accgatctgg
    47221 ataagcagaa acaggtttat gtcaattgtt cccgttacag acggaaataa aaggaaacta
    47281 ccacaaacta ttatcatcag gatcttatct agtacagaca accatgcatc tgttctgaac
    47341 cattgttgtg cagttataat acttctgaaa aatagaaaaa gtgatgtcag tacctgtatg
    47401 accactacac tccagaaaat atcccattgc ccgatacctg atataaaagc ggcaaaaaag
    47461 accaccacta cgtataatat aaccagcagt agcttgatgg ccacaaaact tccggcaata
    47521 ttgctatgac tttctttagc cgcggctaat tccaggttaa taaaagccga aaggccccaa
    47581 tcaagcagaa aattaaaaac aatagaaaat ccgagtaatg aaaaatataa tccataaact
    47641 tctgtacctg taatattctg tacggacctg tcgataccaa aaatccatac aggtttgatg
    47701 attatattaa gaataatcag cagcccaagt gaggaataaa atcggggtgt ttctttcatt
    47761 aacatgatat tcatgatcct ccttaagtag gaacatgcag gttacatcac ttttacattc
    47821 cggctgaaac tttcctccag cgaaataaat gtttctgttc gttcaatacc ttttatcttt
    47881 tgtaactggt cgtgcaaaac atacctcaat tgagtaatat ccttacatac tatctctgca
    47941 aacatgctat agttacctgt tgtataattt aaccgaacaa tttccgggat cttttgcagg
    48001 tctttggcga cagtatcata cagtgaactt tccttcaggt atatcccgat aaatgcaatg
    48061 acatcatagc ccagttgctt aaggtctgct ttcagccttg tacctttaac caccccggct
    48121 tcctgaagtt ttttgatacg aacatgaatg gtaccacccg atacaaataa ttttttaccc
    48181 agatcagcgt aagagatttc cgcattctcc atcatatgat gtatgatctg caggtccagt
    48241 ttgtcgagat ttaattttga agccatttta tataccaatt tttgaattta tcaatcaatc
    48301 aggcaaatat ttaaatattt tctatacttt caaaatattt attagataat ttggaatgaa
    48361 ttgggttact gacctatatt tgttccatca agctcttata ataatactgt ggggtgatga
    48421 aattgcgaaa gcatggcaga catgccctct tgtctcgggg gtggggaaaa caggataaat
    48481 atagcataga gccgttttgg tagcaatacc ggaacgacca gggtcgacca ctgaactttg
    48541 tgctacaact aactgccccg tggaggttcg atccctcccc ctacagctga atcagtttta
    48601 aactgaaact aagcctttgt aaatgaatta tttataaagg cttttttatt ttcatcaggg
    48661 ccggcaaaca atcttttaag cgccaaatcg gtaaccaatt cggtaacctg tttttccttc
    48721 tttaatcttt aaaaaaccat gtatggcaat cactctaaaa gtgttgccct ggctgtaccg
    48781 ttctaaaacg aacgcagaag gaaagactcc aatctatctc agaatgacat gtggaaatat
    48841 gagggtggag gtagctaccg gctacttcat taaagagtca gaatggaaca ccaggtcaca
    48901 cactgtaaat tcaaaagcac cacaggcaag gcagatcaat gaacatctgc aggcattacg
    48961 ttcaaaagca tttcaattat ataaccagct agtcgcacaa gaaaagccat tcacattagc
    49021 tgttatcaag aacaaactca cgggtaagga taaaaatcaa acaacattac ttaatgctat
    49081 tcaataccac aatgaattag tcaagaaagg gattggcaga aattatgcca taggtactta
    49141 taaagggtac ttatggttta cagataagat caaatcattc ctgatgtatc agtataaacg
    49201 ttctgatata ttattagcag aattgaatca ccggttcctg acagaattcg agcattatct
    49261 atatcatcat ttgaataacc aggttaatac ggttcaaaag aatataactc agctgcgaaa
    49321 gatcatcaat ctttgtatag atcttgattg gctggacaag atgcctttta aacgcattaa
    49381 agcaagacct tatacaccaa aaagagatta cctgaccaaa gaagaacttg agactattca
    49441 gaacattgaa atctctaaca agaggctttg tacaatccga gataaattca tctttcagtg
    49501 ttatacaggt gtagctttca ttgacctgaa taatcttacg gcgtctaatg tggtgaaagg
    49561 aattgacggt gaggattggt taattcttaa ccgccaaaaa acaaaatccc gctcaacagt
    49621 accgcttctc cccattccaa aagccctgat caataaatac agggatgaaa ccagggagac
    49681 cttgttccca atttgctcaa atcagaaatt caattgttat ttgaaagaaa tttccttgct
    49741 attaaagatc aataaacgca taacgaccca tactgggcgc agaacctttg ccacgagtgt
    49801 aactctcagt aatggggtgc caatagaaac agtaagccgg atgctagggc actgtgacct
    49861 caaaaccaca gctatttatg ctaaagtggt tgataccaaa gtttctaaag acatggaacg
    49921 gctaaaacat gaactcgatg gcacagatat aaagaaagcc agaaatggat caagctagca
    49981 ggtgcatata acgatcctca ttatatttat agtaagaaac gccatttact gcttatgccc
    50041 agccgcttta ctaccattct cgaattcctg tcggttcaaa aagtactgct agaaaaagta
    50101 cgaatattag aagaggtatc agaaaatcct gataacatta caaatattga caggcaaaat
    50161 gttcgaataa caatgttgca tctagatgat gaaggaaagc attttgaaga aacctattca
    50221 cttgatgaca tgttgttaag cgagtttaaa aactttataa caaagaaaac tgtagatgaa
    50281 ttaagggtgt tgaaattcgg atccaaagaa gggatattac cgataaaagc ctcagattat
    50341 gtcaacactt taaaaaacct ggagactacc attcgtcagg gagttaaaca gctccaggaa
    50401 tccggtcttc ctattccagt tacattggcg gaaatcaccc ggttttgcaa gctgctcgag
    50461 aaaaggaaac ttgaatttat tgacgagcaa gtttcctcac agccaataaa tgacaactat
    50521 ccttttcgtg gctataggtt cactgacccg aagattaaga aaccgttaga attgcttgac
    50581 cgaatagccg actacttaat acaagaaaat ctcattggtc caaatagaaa aacgtctttt
    50641 atttcccttt tcacggaaag ctgggaccag actagaattc cctggaaagg agacccaaag
    50701 gagttctttt gcttttatgc aacattgttt tgctcatccc gatttcaaaa aaaaccgctt
    50761 atcgaagtca agggaaacaa actaaagaaa gaacaattac ttgattattg ctttctggta
    50821 ataaatcctg agacagacaa accaatgagt aaagtggaat tgcgtaatta tgagtcaaaa
    50881 ccaaaatcaa acaaagtata taaaggcatt gcagatatca ttgaagatct gctagatata
    50941 taacttccac aagcccgctt ccgcaaactt gcttccgcaa ataccccgtt atgaggaata
    51001 tgaagcatac aaacttccca agatttgtgt ggtcaactat tctctaattt aaacccacac
    51061 aacaatgatc tgcttcctaa acacaaaagg acagtgcccc acagatgagt tgccagacct
    51121 gataaacaac ttcaacgcag tacccctaaa catcgggtat gtaggaaagt ttcgaaacat
    51181 gaaagtattc atcaatcaaa atggtgggta taacatcaag ggatcagtct ggaaatatca
    51241 attgggaaac aatattacgc ccctggaact accggcatat gaagaggcaa ttaaggacct
    51301 taatagcctg acatttggca cctatatgga aagcaaggtt atcagctatg agttcggact
    51361 gaatattaaa actacccacg caccattgca ctacatgatg caactggtaa gctctaaatt
    51421 gaataataga agcattgact tttcgcctca attctccaat ggaaatttat ggtgtatgag
    51481 atcgacaggg atcgaacggt atatcaagtt ttatgacaaa acctgtgagg ccaaactgtt
    51541 gacaaaccta ttacgaatag aatacgttgt aaaatcccca aagaggatat taagggcgaa
    51601 tataaccgtg aaagatacat tgagcgagtc cttcattcac tacaactgcg atattctgcg
    51661 ccaattcacg gagaacatga tcttcaaggc agactacgat ccggcttatt tagggtcact
    51721 aaaacccagg gacatgtggc gctaccttgc ttacatgatg agtaagcaac ataatcctcg
    51781 actcttaata agcactttgg ggcttgaccc ggtatctaaa tcaagagagt ataaacacct
    51841 gaagagagta atcggagcac ataccgaagg ggaactgaaa aaggagatat gtgaaaaggt
    51901 caatcaagca ct
//
LOCUS       DAORTI010000010        51519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_370_length_51519_cov_6.413920, whole genome shotgun sequence.
ACCESSION   DAORTI010000010 DAORTI010000000
VERSION     DAORTI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 51519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51519
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_51519_cov_6.413920"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLO70_02760"
     CDS             complement(<1..1241)
                     /locus_tag="PLO70_02760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQZ73402.1"
                     /translation="MAIVDLVMPKLGESIMEATVLKWHKKPGDSVQMDETILEIATDK
                     VDSEVPSTAAGVIEEILFNVNDVVPIGTTIARIRTGADEPVVSSPVASAPVQETVNMP
                     AQEAVVVETISPQTSNIKLQTSNGNINRFYSPLVLNIAASEGVSMTELEAIPGTGNEG
                     RVTKKDILQYVGGRKSGGQQPEARSTSSEMVIPLAKVQPDQSASSSQQLTTYTGNVEI
                     IEMDRMRKLIADHMVRSKQTSPHVTSFTEADVTNLVQWRDKVKKEFEKREGTKITFTP
                     LFIEAIVRCIKKFPLINCSLDGNNIIIKKDINIGMATALPSGNLIVPVIKNADQLNMV
                     GLAKQVNNLADAARNGKLKVDDTQGGTFTLTNVGTFGSLMGTPIINQPQVAIMAVGAI
                     KKRPVVIETPAGDSIAIRHMM"
     gene            complement(1379..2647)
                     /locus_tag="PLO70_02765"
     CDS             complement(1379..2647)
                     /locus_tag="PLO70_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HQZ73403.1"
                     /translation="MKSNSVNASIITIGDELLIGQTIDTNSAFIAQELNKIGVWVRRR
                     LAVGDVYEEIWQALDEESRQSDIIIITGGLGPTADDITKPLLCKYFGGKLVVNEEVLA
                     HVNYLFEKVFRRPGPMLERNLKQAEVPDVCTVLHNARGTAPGMLFKRDGKVFISLPGV
                     PHEMKGLIVNEVVPYLLSAFKMPAIVHKTAFTAGQGESMIAELLKDFEPSLPSHIKLA
                     YLPNYGMVKLRLTSRGDKKEEVEKEMIPYFEKLQELVKDFLITNEDEGLEIVIGKILK
                     AKSKTMGTAESCTGGYIAHLITSVSGSSAYYKGSVVSYANEIKENILGVKQETLLSVG
                     AVSEETVKEMVNGAISKLNVDFALATSGIMGPDGGTDDKPVGTVWIAAGNKEKVDTLK
                     LNLRFDRQRNIGMTATNALNFLRKFILANS"
     gene            2707..4092
                     /gene="dacB"
                     /locus_tag="PLO70_02770"
     CDS             2707..4092
                     /gene="dacB"
                     /locus_tag="PLO70_02770"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HQZ73404.1"
                     /translation="MKKVFLLVTGCWILVTGVYAQLVSQRLQNAFQQFESDSQLRHAI
                     SSLYVIDAKTGQVVFDKNSQIGLAPASTQKIITSVTAFELLAKDFRYKTEVGIIFGEK
                     SSSIYLKPSGDPTFGSWRWSATEEAFILDKLVNRIKPHIKSTDSNVYVNQKGWDTQSI
                     PDGWIWQDIANYYGAGAGVLNWRENQFDLLLKSGSKIGDLVEVVKTNPELYSYKIHSN
                     ASSAKAGSGDNAYLYFPVSGNEGIVKGTIPINENKFQISGAMPYPPIQFIETLRDSLF
                     GKNKPYNHLSSIFLSEDPKVSHKIFYTHYSPSLDSIIYWFNKKSINLYGEALIKTFAY
                     EKQGFGSTDSGLAIVKKFWKEKGIDEDELNMYDGSGLSPLNRVTTHAQVEILKYATTK
                     DWFHYFYNSLPLYNGMVMKSGTISDVKGFCGYHKAKDGKEYIFSFLVNNYRGKTSAVV
                     NKMYKVLDALK"
     gene            4170..4598
                     /locus_tag="PLO70_02775"
     CDS             4170..4598
                     /locus_tag="PLO70_02775"
                     /inference="COORDINATES: protein motif:HMM:NF040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HQZ73405.1"
                     /translation="MAEQNFKNHARLVPGFHGVTFLLIVTGLVGSVVNLFHADAATHY
                     SAALLVVVFLALLGVAWYARVFALKAQDRAIRAEENFRHFILTGKPLDKQLHISQVIA
                     LRFASDEEFSALAKKAAAEKLSQKQIKQAVQNWKADYYRV"
     gene            complement(4599..5609)
                     /locus_tag="PLO70_02780"
     CDS             complement(4599..5609)
                     /locus_tag="PLO70_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73406.1"
                     /translation="MENFTELPGEMKGNETAASPEEVIVIPPKYQKPEEKSNIWLRSF
                     ISLAAYLVLGYYIFPSYQILLLITAIVMIHELGHFFAMKFYRYNELGIFFIPLLGAYV
                     SGSKRDVSQLQSAVILLAGPLPGIIIGAVLFLVDQYSSGIYFLNFSLSRIGLLFIVLN
                     LINLLPIYPLDGGQLLNRVFLDEESFWSKVFVFLSAAFLCLLAINLPFYPLLLFPAMM
                     LLRLAGEKKVTTIEKKIEAAGIHTDLEYEDLRDEDYWKIRNILIEEHPAFKDVDPSPP
                     YEYSHKEEKIMAMIQSLLHRHLIQDVSVAGKIFIFLIWAAAIASPWLLNMDMSFFDRF
                     RF"
     gene            complement(5619..6029)
                     /locus_tag="PLO70_02785"
     CDS             complement(5619..6029)
                     /locus_tag="PLO70_02785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQZ73407.1"
                     /translation="MFTTETKIRVRYAETDQMGVVYHSNYFPYFESARAESIRELGFT
                     YADMEKMGVIMPVVDVHCRYLRPAKYDDLLTIKTMLKELPTHHKIEFHHEVFNEQNEL
                     LAVGKIILYFMEAKTMKKTIMPELLLNKLQSYFS"
     gene            complement(6046..6453)
                     /locus_tag="PLO70_02790"
     CDS             complement(6046..6453)
                     /locus_tag="PLO70_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQZ73408.1"
                     /translation="MKTFFSTKYSDKSISLSLLLLRLSAGGLMIPHGYDKLMNFAVKS
                     ARFADPFHIGSTTSLAMVVFAEFFCAILIVAGLMTRLASIPLIIVMGVALFKSHNGEI
                     FGEGEKAALFFIGYMAILLGGPGKISVDKLIGK"
     gene            6672..9710
                     /locus_tag="PLO70_02795"
     CDS             6672..9710
                     /locus_tag="PLO70_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73409.1"
                     /translation="MKNQSLLSLAIFLSVSVFAQQTRFYSDPEEKFKEAKEYFQKEQY
                     SLAYPLFRELRQSVKETDRANTAVTVQEIKYYTIVCGLKQGEGRSEEEAQEYIEVEKN
                     DPRVQMMNYHLAEFYFRTENFTEAANRYEQTNIANLSNREIADMKFHQGYSYFTLQRF
                     AAAKPLFNSIRSIKDDPNYLDANYYYGFLAFRDRQYREALEAFKVVENEKNYSTVVPY
                     YIAQIYYVQGRKDEAITYIQNKLQAGSSSQYYDLELKQMLGHAYFEKKEFAKALPYLS
                     DYVSRSKKVRREDLYELSYSYYQENQLSNAIDGFKQLSGKDDSLSQHAMYLLGDAYLK
                     TGQKSNARNAFLFCSSNSSYPEQKEISKYQYAKLSYELGYQDEALNSLSSFLADYPNS
                     KYNTEAKELMIDVLANTNNYKDAQSLLESLAKPSENAKRLYPRILYGRSTELINDGRL
                     AEADALLDKALKDPNNGSVLPLLNFWKGELAYRNNKIDEAIKFYNAYVSGGSPTSGEA
                     NPANASYNLGYCYLRKENYPVALTFFEPLGRNPSLSSSSFVQDAYIRTADCYFMQKDY
                     AKAKTHYDNVIKFSWPAEDYATFQIAMLAGVRSSTEKISLLNTMMRKFPASSLVTDAN
                     MEVANTYMGDERFREAIPFLGNVIKNSNNASLIPQAYLKLGTSYYNIDNNAEALKQFK
                     ILVDKYPNAPETEDALDNVKTIYIEDGKPGEYANFMRQAGRPLSMNTEDSLTYAAAEN
                     QYDDQNINAALTAFNDYLQKFPDGAYALDANFNRAEIYNSKKDWNNALVGYDEVAVNA
                     PNKFAERAVLTAARINFFELKNYQKAEGYYGQLKQITASQENKLEAMRGLLRCQYQLQ
                     KWTEAVDNAKELTSAKGSSADDKSLANMAIAKSYQVAGDYGTAITNFKSVVQINKAAL
                     AAEARYEIANCYYQLNKLTDAEKSAFETINKSGSYDYWVTKAYILLGDVYFKQKDYFN
                     AKATFQSIVDNTLNTELKNEAQAKLSKVVDEESESSKVGN"
     gene            9741..11393
                     /locus_tag="PLO70_02800"
     CDS             9741..11393
                     /locus_tag="PLO70_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73410.1"
                     /translation="MKQALYKIFFLSVGIFSVGISFAQKDTTKSGGVTIVSSFKPVLR
                     EAAKINFNASPPAADTTKAKLNYDIPNQNLLLGYQPGSLKPLALSIDSVVKFDNSSYI
                     KAGFGSLRTPYVQAGISFGDGETAGLNIYAKHVASESKRDFQKFSSTNVRLSGYFKSG
                     NNLQWDAGLGMKRDRTYKYGYLPATLVFPTDSLKQSFQTISGRIAFHNINKTEFGLTY
                     APEIKIDVFSDNLKNSESNTIVNAPLQKTIGKEFAVNLGITFDLTRFSPENKTAVNNT
                     MYYISPSVLYKTSQINAQVGIRPSWDNGKFKMFPNILADISTTDKRFTFQAGWTGYIR
                     KTTYQYLAGQNPWLWLPGTLRNTWIEERFAGFKGTAGDHFSYAAKVGFNKLNNQPLFI
                     NDTSAAGDGKSFNVVYEDKMNVVNFSGEFGYNAGEKFSMITGLIFNQFSGFQSQAKAW
                     GMIPLELKTNLRLQIIKDLWLKTDLFAWSGSKYLKKDNTPGKLDGAFDLNAGLEFKIT
                     KNINLWTQFNNLFNKEYQRWNQYPVYGFNFVGGVVFSFDQKN"
     gene            11396..12040
                     /locus_tag="PLO70_02805"
     CDS             11396..12040
                     /locus_tag="PLO70_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73411.1"
                     /translation="MYSELYQYLIQHKQLPVPGIGTFLLERKAATINFPEKRIDPPTY
                     TISLQSLLNTPSNSFFNWLGAALHVSDRDAVIRFNDFAFDLKKQITGGDIINWSGVGQ
                     LSKGLGGDVKFVPLDCQNSLETFVPADKVVREKAEHMVRVGEDERTSAEMTEYFNQPE
                     AATSYWWAYALVVAVLSVIFIGWYFSKHGVDISSAANGKRLIPAETIDTRKVLP"
     gene            12090..12986
                     /locus_tag="PLO70_02810"
     CDS             12090..12986
                     /locus_tag="PLO70_02810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73412.1"
                     /translation="MKELIHYTHCPVCGSADIVNILSAKDHTVSGETFIVAGCNACSF
                     HFTQDVPDAASISPYYKSENYISHTNTSKGLINSLYQSVRKRTLKQKRKLIERVAGIT
                     KGNLLDVGSGTGAFAGEMKKNGWSVTGLEPDEDARNVAKQSFGVDLSDIRGFYELPNE
                     TFDAITLWHVLEHVHDLQGYMAKLQLLLKENGKIFIAVPNYTSKDASVYKEYWAAYDV
                     PRHLYHFSPQSMKVLIEKHGMKLLQYKPMWYDSFYVSMLSSKYKNGKTNLVAAFFNGL
                     LSNFGASADVKRCSSVIYIIGK"
     gene            complement(12987..13775)
                     /locus_tag="PLO70_02815"
     CDS             complement(12987..13775)
                     /locus_tag="PLO70_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HQZ73413.1"
                     /translation="MKKIFLIATLSFFVACKNEDKPGTVTPPANAPKSMSYSIVATYP
                     HDTSSYTQGLLIYNGVMYEGTGNYGKSKLKKVDLKTGKSLQEISLDKKFFGEGVTILN
                     DTIYQLTWKEKTVFVYLLKDFKKIKEFPIEIEGWGLTTDGKNLIASNGSGDLFYYEPS
                     SFKLVKTQTVTEAGSPSFNLNELEYIDGYVYANQYTYPYIFKINPGTGEIIAKADLTQ
                     MWDRIKAIDPDAEVPNGIAYDATAKKIYITGKWWPELYEVQFGQ"
     gene            14073..15299
                     /locus_tag="PLO70_02820"
     CDS             14073..15299
                     /locus_tag="PLO70_02820"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQZ73414.1"
                     /translation="MKAGVNIIFLLLGLISNQLIAQMNCVTSSYAQEKLKNNPALNNS
                     INGIERFTRLVLENSSASRIESGVIKIPVVIHNLYHTPGEKISDAQVASQLELLNKCF
                     RRKNADSVNTPAVFKGLAADCEIEFQLATSDPRKRATTGIIRKYTPVSKWIMDDKMKF
                     SAEMGDDAWDSKSYLNIWVCNMEKFAGYASMVGGPEDVDGIVMGIAAFGGTNKTIVHE
                     TGHWLGLKHIWGDEYCGDDGVNDTPKQASYTTGCPTTIRVTCGNGPTGDMYNNYMDFT
                     NDACMNLFTEGQKARMRALFVGGAVRNSLLSSKGLDAPLIFEAPLPDEDPKWLEPKMY
                     PNPATTTVTLDLSYDIRWIGKTIFVTNLHGQSIMNVMITAKFQQIDISRLQPGLYFLA
                     AKNDQGLSIKMKFVKL"
     gene            complement(15450..16853)
                     /gene="lpdA"
                     /locus_tag="PLO70_02825"
     CDS             complement(15450..16853)
                     /gene="lpdA"
                     /locus_tag="PLO70_02825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQZ73415.1"
                     /translation="MVYDVVVLGSGPGGYVAAIRASQLGLKTAIIEKESLGGVCLNWG
                     CIPTKALLKSAQVNEYIKHAKDFGIEAAGTPNFEAVIKRSRGVADKMSKGVQFLMKKN
                     KIDVIMGFGKLVSKGKLEVTDKDGKKQIVEARHTIIATGGRSRQLPTMPIDGKKIIGY
                     REAMVLPQMPKSMIVVGSGAIGVEFAYFYNSMGTKVTIVEFLPRIVPVEDEEISKELE
                     KQYKKNGIGIMTSSEVTAIDTSGNGVKAKVKTATGEVELEADVLLSAVGVSANIEGIG
                     LEELGIKTDKGRIMVDKYGQSNVPGIYAIGDCSPGQALAHVASKEGIVCVEAIAYNEK
                     KYHHQPEAIDYNNIPGCTYCVPEIASVGYTEKQAKDAGYEIKVGKFPLSASGKASAAG
                     HTEGFIKVIFDAKYGEWLGTHMIGYNVTELIAETVTARKLETTYHEVLNAIHPHPTIS
                     ESIKDAIEVAYGEAIHI"
     gene            17169..17405
                     /gene="rpmB"
                     /locus_tag="PLO70_02830"
     CDS             17169..17405
                     /gene="rpmB"
                     /locus_tag="PLO70_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQZ73416.1"
                     /translation="MARVCQVTGKTPIGGNKVSHSNIKTKRRFLPNLQKKRFYLAEED
                     KWITLKLSTTALRTINKNGLLSVVKELRAKGEKI"
     gene            17427..17609
                     /gene="rpmG"
                     /locus_tag="PLO70_02835"
     CDS             17427..17609
                     /gene="rpmG"
                     /locus_tag="PLO70_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQZ73417.1"
                     /translation="MAKKGNRVQVILECTEHKTSGQSGTSRYITVKNKKNNPERLELK
                     KFNPILKKVTVHKEIK"
     gene            17612..17782
                     /locus_tag="PLO70_02840"
     CDS             17612..17782
                     /locus_tag="PLO70_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HQZ73418.1"
                     /translation="MAKAAKTAIKKDPKAAAEAKNYSKVIKAVRSPKSGAYTFKEQMV
                     HKDKVKDFLAQK"
     gene            17925..18887
                     /gene="ftsY"
                     /locus_tag="PLO70_02845"
     CDS             17925..18887
                     /gene="ftsY"
                     /locus_tag="PLO70_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQZ73419.1"
                     /translation="MGFFNKLFGKKEKESLDQGLQKTKEGFLSKITKAIAGKSTVDEE
                     VLDNLEEALVGADVGIETTVKIIERIEKRVSKDKYLNSSELNRMLQEEIEQMLVDAPE
                     SNSYAFDSDLPTRPYVILVVGVNGVGKTTTIGKLAYNFKKAGKEVLLGAADTFRAAAV
                     DQLTIWSERVGVPIVKQAMGSDPAAVAFDTVQSAVSRHTDVVLIDTAGRLHNKAHLME
                     ELSKIKRVVQKTIPDAPHEVLLVLDGSTGQNALEQAKHFTAATDVTALAITKLDGTAK
                     GGVVLAIANHFKIPVKFIGVGEKMEDLLVFDKHEFVDSLFNLEK"
     gene            18975..20294
                     /gene="rimO"
                     /locus_tag="PLO70_02850"
     CDS             18975..20294
                     /gene="rimO"
                     /locus_tag="PLO70_02850"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQZ73420.1"
                     /translation="MKTKKLHKDKVNIITLGCSKNMVDSEVLSGQLLANDIDVVHENK
                     KLDHNIVVVNTCGFIDKAKEESINTILDQVELKRRGKLDKVYVTGCLSERYKNNLEAE
                     IPEVDAYFGTMELPLILKQFEADYKTELVGERLLATPQHYAYMKISEGCNRTCSFCAI
                     PLMRGQHVSKPIEELVKEAEGLVKKGVKEVMLIAQELTYYGLDIYKKRMLGDLMHRLA
                     DVKGLEWIRLHYAYPSKFPLEVLDVMRERENICNYLDMPLQHAANNMLKAMKRQITRE
                     EMEELTIAIRGKVPGICLRTTLIAGFPGETEDDVEELKGFLQRQRFDRVGIFTYSHEE
                     GTSAHDLADDVPAEEKERRAQEIMEVQQEISFELNQEKVGKVFKTLIDKKEAGRYLGR
                     TEFDSVEVDNEVVIHSVKKLPIGEFVNVKITKAYDYDLEGEVVNEGS"
     gene            20342..21202
                     /locus_tag="PLO70_02855"
     CDS             20342..21202
                     /locus_tag="PLO70_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73421.1"
                     /translation="MKKYMSVGGVLFLSVFAFSQGQESQVNAFEVHSKGSQVFSVLAL
                     KKFALPLPERAIISESYNGTRLFSVSVHGSKLNGNWQSWYATGVLCDSGRLINNLPDG
                     EWKHWNEKGELLAIRHYSADKFQRVMQEMLRYNPKRSFFYLSELYQKDKQAALNYLTA
                     AYSFPSGRSAPVHSLKQLVISNISKSDSYRPVFEQSLQDGLYMNFFPGGSIRDSGYYK
                     NGVRTGKWVHRDSADGPWHLGAYQHSIKIKEWKYYDKNNKLLELIVYDNRGHINWRKK
                     INRQGNHEQH"
     gene            complement(21251..21769)
                     /locus_tag="PLO70_02860"
     CDS             complement(21251..21769)
                     /locus_tag="PLO70_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73422.1"
                     /translation="MNAAQIHLALNHAPLFLSIIGGGILILGMIKKNESFKNLSLYFL
                     IAAALFTVPVFLTGEGTEELVENLPGVNETAIEEHEDMAKISLIIISITGAISLLAIF
                     LKKNTKMAKLLFTGALLFSLASFGFMAQTAHLGGLIRHSELQNGNSTNTNPKEKDASG
                     KEIKEQEKDDDD"
     gene            complement(21843..22238)
                     /locus_tag="PLO70_02865"
     CDS             complement(21843..22238)
                     /locus_tag="PLO70_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73423.1"
                     /translation="MKQIQVLRLTLFTILTGFFLLSTTELHQFLKLPVLLKHFQVHKK
                     QDKSLSLLDFLALHYSQDHPIDNDDSEDNQLPFKNFEATSHVDLTIIPFRFETEELVS
                     AIAGIIRLYHPEGVPCSRSYAVFHPPQAS"
     gene            complement(22321..23418)
                     /locus_tag="PLO70_02870"
     CDS             complement(22321..23418)
                     /locus_tag="PLO70_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQZ73424.1"
                     /translation="MPDLRSIQTEVDATFLYKKLADNEENKEVADIFRQMSEIESSHA
                     DAFLKKLNLSPDKMPLPSFRAKTLNRIGKIFGYDYVLGVLMDTEKSLSNSIINLKKQT
                     KQPITGTEVNHIKVLRNIIEQSEISGSSIARFESRHRSVGGNALRAAVLGGNDGLVSN
                     FSLVMGVAGATAGQEGVLLAGLAGLLAGALSMALGEWISVKSSQELYENQMQIEMEEL
                     ETHPENEMKELELIYKAKGIPAEQAKEMAAEIIRNPEHAHELLVKEELGINAEELKGS
                     AFEAALYSFFLFAIGAIIPVFPFMFLSGINAILLSTAASAVGLFIIGAAITLFTGKSI
                     WFSGFRQVIFGLAAAAITFGIGKLIGVSVAG"
     gene            complement(23451..24194)
                     /locus_tag="PLO70_02875"
     CDS             complement(23451..24194)
                     /locus_tag="PLO70_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73425.1"
                     /translation="MDNTETLNKLINRPQRVVRNVIIIAVLMIAGAFITGYSGLEGMD
                     GGYALIVIFGFLALSAVVTALVYIPRAREFKRLTSNLRPLAHWTYTDQEWDDFIKEDL
                     KEMIAVNKATLRLVIIISIVVCALLLLVYRDNLFIVIIAGIILLLTIVAFLAPRIRSY
                     NLQKGIHEAYIGNTSAFVGGIFHTWTQLGAHLIAVDIYTESKIPVLHIIFEFPTLQAN
                     QQEIIRIPVPAGKMEEARKIADQLKAQIK"
     gene            complement(24208..33597)
                     /locus_tag="PLO70_02880"
     CDS             complement(24208..33597)
                     /locus_tag="PLO70_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQZ73426.1"
                     /translation="MLPLPSMRQGFITILVGILFANTSISAQTQEDLEKMGREAKPFQ
                     FKLKFNGVGAVNPGDAKYATITALLDKYKSQVPGHIQLPFKVQGPEAGQKPVLSPDQQ
                     TLLKDQSTRFFREMAELKLTVQDLQRYKEVFKNKNPMPGDNPIDPKIAWKGEFEGIER
                     TLMGGRTLMIMDVWKQTMIEYFNNHPEAVGVVYGQLDIGSWVKMNVDGLGFAADIDFS
                     TIATDAATNQALHNLFGENLKRASGLGMIPIDVVHTAHGLAGAEVFIGEWGKAFAEVD
                     MLRRGKWKLLEMERNQNGQIIDIKTVEKEGKQLFMEKGMEQELKAQKNDPANSFDPKE
                     RYPELRIDMEPMLSLEMLRHAIHDIEHGPFEGGQKIIKMIKYTERSFFMIKEALKNVS
                     VKDQMLYFNLTLEEQKLMTLTEEVIRNKGDAKRIAGLMEEFSGKKLESNEQVNLIVDD
                     VVQKCKKAMLKNANQAFGYRVKTIAAIENDDQRFEEADKFLKQMEEEFKKGYEKSGTE
                     IPKSMLRAHTILVDLRAGKIPHDMMNARLEELNKLMEEEYKLDKSFVERLIGDPWGRI
                     KNYLVEKGYGPEAVQKILNKFKSVFTENWRQYAPEYAQKTAMSAYEFSSHVTNKLNSF
                     NEHLAKTKTGKVLSSEILNKLDDGIALWDAWMSGKDYKQSAWNVSWTAGTIWAQGKWP
                     FLAIPLGIYNSIQTRSPAPAAMAVTFYLFPFAGQAYMVTNIIDRLVVSQVMDYNFRNH
                     LDRLAVMAITDGQGHVVRFKIPRAFNGLTGSADSLETDPDISPSEPARTLGIKNVFYD
                     PAFMYCPDIKYFESLIPRKTDQFGMYETKHKNLMTLFAFDNEFVGYLVGVKKFKEEKE
                     NGNIEEVAASEISGQRIKMLDSLEIVIEDRLWSAVFSAIESTKRAEKKGVVDELEATI
                     IRIQDSLFMDEWRMKKMDSLSLLLKIRKQIQNDPLYIQALKDKFSAGTAYERVAIPTF
                     ERYIQIYRQILVIQSKIFEIWRPFGVDASKLQEDPMRLVLMGGLSGAPMLTTDPGKDL
                     QLAIQCFEAHTKRASDIRYDLATALGRPIKETEKEDKEHLRILGEYGFGFERLVDADA
                     NGRSPRFIDETGEIVKKMQEYRKAYQDYQARLKEGPAIDVTLKVEGETETTETNPVTA
                     DVIILNEKQQTIPLPAGTTIEWYRYDLATVKDIKVGDGNKYTHDAKEQGTFTYTAKLS
                     RTINNKKTELAKGYWNITVKVYDVNDPSKGIKLKIPALIKQYEIFDVSADIPALLKSR
                     ISDCSWGWETIGDNKNCAGSKIQVNHTYETTDKSGKKFLQDSITIGISFSLLQPGSTR
                     PYSQYVNKKVKYQHMSLAVKATDIWEGGSGANWFRLERKPMKNAPRIPGWSNDKKAIS
                     TASAYANVGLKDDDPMETINNLEELQKYMEKEAKEQAYKQLEVKPISVGDFKGYGLLS
                     APKYDRGGWSDAGYRSAGAGSSFNGYVMKGKKIFEVYWYAGAGGAFDNSDQAWMMSMV
                     KQLAAEATSILTSASFQPDGRLTKSPYTGPKLDGSDYPMVTIEPKIDTIQPSSKVSVK
                     AVIKNDKPDYGPYTYSWTGHVEGNSTNSTAQLVSDRPGKKSITVSVDGTTPPGSATIE
                     YVVAPLKVKLTKVSPATNKIIVGMPVELKADFISTIPAGKKLQYLWQPHPEEKFDPFE
                     GSKNTTKVIFSKPGHKKVWVQVLDKTTAETITMGESDQLEFDVDKPAFNITFTPAKAK
                     VGELVTAKINSIPDKLEDVSYRWMPVPANANLIKESQDGSEITFYAKDAMQIALEVLA
                     VVKGSGEELGNTKAYFNAERYTVKTEGPKVQGPKPMIWKPGIGLVEVDKEIAVHQVIE
                     FSTIVTPQPSGNLTYNWQVTEGNASISNPASRDARVTALETGTIQLKVTVKNNNGLEL
                     GNAIAVFSATISNEMITNGVSQKKAFDEKMQQARQLLKEGKLDEAIKLGEEVKGMNAK
                     EAAPLINELVEACKKGAKDAAYERDFNLAIKRCEQALKMNPTDAAAKTQLDQNKKWAK
                     EWPAVLAKGDELESNISKKNIPAGEKNIAELNKLQFNMPGQMANKWSQQKYNKYVALL
                     KSYDSVYAITRKNWSDQYKEKDFENGLKTLETFKNEWTPTSAAMKEVESSIQLGKTQL
                     AEQKRIYDDFLITKSKFEQGLPIDAKQTPSTIETTATSRFSTNDARQKEMIDFARSMD
                     KRQKEITVNKDKAAQLKKDGQKAEAAGQKEEALGKYKESVALIPDPELDNRIKKLETE
                     LAAIQAKKNKADELWNEGEKLASKKKTKKDGLGKMKESLEWWNSGERIHKVRELEKEV
                     NGSYSKIDISGVWKHGNTETFTFTSSGNGQYTAVEKGFDNATGSLTMIGETGFINYII
                     KNGITGQYILKITADGNTATGKWTDSRNNNGARNFVRISKPETPQPDVTKTDQKKKKK
                     SFNDILDDINKGLGKIDSAITGKKPEEKGVDPADANVEERIFYNGNIGGVSSKPTKAT
                     VFTLSKATYITRIENYHYYNGGKKPGTIAVQSSNGQKYGPWQAYGVIGQGGVQNATWV
                     VQPKMQLPAGTYTVIDSDPSTWSQNGESKGCGFTTVWAPKSGKQPTTEVDKGVNPADA
                     KTEVKVFDNGNIGGVSSGPPNPTLFTFSRATIITRIENYHYFNGGKKPGTIGLRSSSG
                     KIYGPWQAYGLIGQGGVQNAYWAVLPNIEVPAGTYTVIDSDPSTWSYNSQSKGCGFTT
                     IWTSKTNKPLTNPNDPGYTIEEVETGGGVTAEKIFIAGDIDNLGFGFPKGFDVFSGNA
                     TPSHGYPWKINPGDPPGTDRIMVGTSNKSNYDGYSNSTARPENLPQAITLLCPVSNIS
                     VRSAVLQMFVDDFQAAAFGSKFTVTINGRKAVFLETVINSLNQTGPVGKLISVKIPAD
                     FMNEIQSGKLVIYIDDATSGKGDGYAVDFVRLIINPTAYAYTGTITGIIYRPDGQPAV
                     GASISAGGIINTTANSKGEFTLNNVPAGMVSVSASYNNRHHKTITTDLASGKTIKITI
                     TLPAEESTTASPSPTTGQGNDNTAVNNTGILYGTVFKTKEEAGKNNPYDLEGVPLPNT
                     AVTITYIYNSKTITKTVNSASNGKFEFTGLPLNISIRISSRGTTQNKTLTTESIKQYV
                     QFGWDGEITIK"
     gene            33822..35891
                     /gene="ppk1"
                     /locus_tag="PLO70_02885"
     CDS             33822..35891
                     /gene="ppk1"
                     /locus_tag="PLO70_02885"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQZ73427.1"
                     /translation="MASGKYVFLNRDLSWLSFNHRVLMEAADKTVPLYSRISFLSIFS
                     SNLDEFFRVRMPAIFAFSNLESKKISLEEEYPRDLVQQVQATVFEQLEEFGSILTKQL
                     LPGLQQQNIFLYYNEPVRKEHAETVREYFLSRVLSFLQPVFIQKDNQAGFFLENNALY
                     FIIDLVSPEQPEKHQFALLNIPSANLPRFYELPMLGDDHYILFLDDVVRENLQEVFPG
                     FDIQEAWSIKLTRDAEMNLEDEFIGDIAEKIEKQLEKREGGHATRLLFDQRMPEPVKD
                     FVQKYFGLRTEELVPGGRYHNLKDLGGLPNPVKGKIAQINWQAVTHPGFNNHLSIFQS
                     ISEREKLLHLPYHSYNYILRFFNEAAIDPHVSEIYITLYRVAADSHIVNALISAARNG
                     KRVTVFVELKARFDEANNLRWSKKMKAAGIKIINSIPRLKVHAKVALVKRWENEQWKD
                     YSFMATGNFNEATGRFYTDHVFFTTNLEFGKELDMLFTYLQSRTQPVLYGKISFNHLL
                     VSQFNMVKRFNKLIDREIKNALKGKPASIIIKLNNLQERDMISRLYEASQAGVKVQLL
                     VRSICCLAAGVEGQSENITVHRIVDRYLEHARVFVFHNDGEPLCFMGSADWMNRNLHS
                     RIEVCFPVYDVKLKQELLDILDLQLRDNTKAVLFTSDLKNNRITPAEPPVAAQQSIYE
                     YVKKMAE"
     gene            complement(35964..39902)
                     /locus_tag="PLO70_02890"
     CDS             complement(35964..39902)
                     /locus_tag="PLO70_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQZ73428.1"
                     /translation="MSRFFTRLTHLLLFIIFAGILHAQEKNDYTILLNSGKFIPAENI
                     SGLTKNSAEFRQSLFLNKHYVTIQFKALPAQQEKNNLKAAGIELIDYIPNYAYTVSVS
                     ADFDLSSFKSFNLRSVFQFTAAQKTVPALLSGTIPPHAIKQPGYVDLTVITYEKLTVG
                     QITGSLLPLSASILEDMPMFRSFTIRIPQSNMQQLINLPYVQWVEFIDAPNQLENTLG
                     RTLHRVNVLNDGVRNLKGDGINIGIWDEGEISPHLDFSPSGRLNQMEFSSPSQHSTHC
                     AGTILGRGLINQTARGMAPNASLYSFNFSGNIQTEMANAIPAQGLVISSHSYGSTQTC
                     GLNGAGVTYSATSRNTDLNLNNFPYHLHAHSAGNSQTACTGGWSTITASGKSAKNNIL
                     VANITTLEALSGSSSCGPVQDGRVKPEISAFGTSVFSTSTPVNGYATLSGTSMATPGI
                     AGSLALLVQRYKQLNSNTLPPSTLIKNTVLNTAHDLGNAGPDYRFGYGRLNALQAVRI
                     LEENRYALNNVTTGTSNDIIINVPAGAARLRVMLTWNDPAGAANANPALVNNLDLTVV
                     NGATTTLPWILDPNNPATPATQGVDNVSNIEQVTINNPAAGSYTLRVNGTAVPTGPSQ
                     DYALTWSVDQPYIEVIYPNGGESFNPGTNETITWDNAGVTSTQTVEYSLNGGSTWTVI
                     SSSVPAGTTRLSWSIPAGTNTSTALIRVSSGAVSDASDANFKILGTVTGFTGSGVSCN
                     AGEIIFNWNATLNATLYDIYRLDAATGQFVLFVPNIGGNTYTATGLTPNASMWFTIRS
                     KNTTTNAESERSNAINVVVSNGGGGLGAVGAISGLQNICGTPSNIVYTIAAVTGATSY
                     TWAAPPGATIASGQGTTSIAINYNAGSTSGNLSVFASNGSCQTAPSTLPITVGSTAVA
                     APTSGGDQSQTVCPGNPVPTLTATATVPAGHTIIWYNAATGGSTVTNPTLSIVGTITY
                     HAASVNISTGCESTTRTAVSLTINSVAQASATAGGPTTFCQGGNVVLTANAGTSYNWS
                     NGASTQAITVNTTGTYTVTVTTSGCTTTSSAINVTVNPLPTATVTAGGAVAFCQGGNV
                     VLTASAGNSWNWSNGATTQSITVNNSGSYSVTVTNASGCSTSSSATAVSVSPSPVVSI
                     SASPYTRLYPGLTTTLTANINPPGTYSYTWFKDGVAVSGAVSSALTGIDLDKLGSYTV
                     TVTNTSGLPCSNTSAAVAITDSAFAKLFILPNPNNGDFDVVYHSTGANSYTLRIFDAK
                     GAIVYNKAYSIIAPYQRMDVDIRKHGKGIYQLVLTDRNGKRIAVGKVLIH"
     gene            40058..40960
                     /locus_tag="PLO70_02895"
     CDS             40058..40960
                     /locus_tag="PLO70_02895"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQZ73429.1"
                     /translation="MSFEWKGIFPALLTPFTKDDELDIPMFEKNLQAQLEAVVHGIII
                     GGSLGEASTITEKEKEELTEFSVEYLNGKIPVIVNIAESTTKEAVRQAANAKKWGADG
                     LMLLPPMRYKTDDRETITYFKAVARSTELPVMIYNNPVDYKIDTRPELFDELVDCVNI
                     TALKESSRDVTNLTRMKNRFGDRFKILCGVDTLAMEELCLGADGWVAGLVCAFPNETA
                     AIYNLVKAGKIEDAINIYRWFMPLLELDIHPKLVQYIKLAASQTGISNEYVRAPRLVL
                     EGEERERILKVINEGIAKRPRLDI"
     gene            41023..42504
                     /locus_tag="PLO70_02900"
     CDS             41023..42504
                     /locus_tag="PLO70_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HQZ73430.1"
                     /translation="MYKDTTIEEINSVMEQAWKAFHLYRKLSLKQRAGFMRAIAFELE
                     ECGDTLIQTAMQETNLPEARLRGERARTIYQLDSYALACEKGDWLEARIDTAIPDKNP
                     PKPDIRKMLVPLGPVVVFGASNFPFAYSTAGGDTASAFAAGCPVIVKAHPAHPQTSQI
                     VADAILNAAVKSKMPDGIFAHIHGIANEVGKALVTHTYTKAVGFTGSYAGGKQLFDWA
                     NQRKEPIPVFAEMGSVNPVFLLPEKLQSSADEIATMYAGSITLGVGQFCTNPGLIIGI
                     ESKALQTFIHDLGKAIQQIVPGTMLHPGIANAFAAKKEESLLQPDVHLVAESAIAPTE
                     KQGQPTIATVDGRTFLSNPVLHKEVFGPYSLVVRCADMEEMIEVAKHLEGQLTSTIMA
                     TTADINNNDELVEAVKNICGRFILNSVPTGVEVCLSMHHGGPFPATTDARFTSVGADA
                     IKRFARPIAFQNWPDELLPDELKNANPIGIWRMVNTEPGNTAL"
     gene            complement(42517..46023)
                     /locus_tag="PLO70_02905"
     CDS             complement(42517..46023)
                     /locus_tag="PLO70_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ73431.1"
                     /translation="MAKRLFVFLFTILLYGQVQGQMRPVSKTPFNKFDSTKTENFFFE
                     IKRVNRVKPDSAIAYISYGKKDGLFKGAAGTIFTTRVANEKNNNNTRETAIYLGNAEV
                     IAITDSSAAVKISVYNKFLDEKIYGGDLVELSAYTMPGIKKNIFYELAKLNILFLDNN
                     RQPIFTIADAIRNTNPDFETDFMQLYTDEILDFKDDVEQYIDSSLKVPHTKGPFKGRN
                     LVDMFKTASYYDLNSFFHFVRKYPGKYMGNEWKINETFATWVINYGPQGERNREWLLP
                     LIEKTNIDQLGDLVKKSAAYIQSDSLIEWTERVNALQTEEKNEAALQLCNKLIFVAKA
                     LKNTVAEHEFYYTRSFLAEANGDSKAALADVLKAYSYNPKHINYRYQLASLYGKAEQF
                     DKCFQLYDELLKELPGNINITGNYGWYKLYAGKVDEAIPFCRTAYFGDTTNVAFTVNY
                     AHTFLIKGNTDSARYYYQKTLDNLYSPADYTTGPKTDFDFLFKKGWNRKAVAEMADWM
                     DKEFEEKYYAITKGNETWKKARQQYDEKNYKAATSLWKEYITLYDKSKEPPVSSIHNA
                     HNWIGVSYYYARMYDSALYHYQAALKIAREKLSLQRNIYTTGEDDYTVSDYDRIYNFY
                     TNTGNKTKAEQYKILYNAELQKVTELYATPALHIIALNGSSRPGEYSGKNNAGAFFHS
                     ISGIKDKENQKSFNKLVNGSEINREKLTGFIDLVRINSKPEDIFVFYYSGENNSSNGQ
                     HYLDFNTKDTTSGRIAITELMDHIDLVYANKKMVITDLPNPSLLNLITTKYANTGNNS
                     AEIIFLSPGIETPVQENGVSLFTNQLALSLNNLKEKGSFTAKDFIDKASFAIGRGEHY
                     LPVLSFTNSKDFVLFENEKNDSAATINMAGTRGLIVKENATSNNDPAVTGSQKNYALL
                     FATDNYMDAGFNKLVNPINDATALESLLINEFGFEVKLVKNPSKDDIEKWLSEYRDNK
                     SYGPNDQLLIFFAGHGVYYDKAKMGYLVAKDSRVDDQTHKTYLSYSDLGNIYLKNISC
                     NRIFLVLDACFAGSFFDNNTVRGTPDVDAKNLSTLMKNASNKRFYKGISSGGKQYVAD
                     GKPGQHSPFAGSFLNVLFNKAMNKSFVTADEIIGEIKSNPPASTAICEGNFHYSDPFS
                     HFIFELKRTEKVSTIKKDEQKQ"
     gene            46278..46856
                     /locus_tag="PLO70_02910"
     CDS             46278..46856
                     /locus_tag="PLO70_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HQZ73432.1"
                     /translation="MKQDELLAKRSGNKCELCEATEHLKVYEVPPATGNMDDAILLCP
                     KCLAQVEKKEELDSSHWQCLTTSMWSEVPAVQVMAWRMLNRLRNESWASEQLDMLYLD
                     DEKLAWAKASGDHENDASVDLHKDCFGAILQSGDNVVLTKSLDVKGSSVNAKVGTVVR
                     NIRLVPGNTEQIEGKIEGQQIVILTKYLRKQG"
     gene            complement(46917..48323)
                     /locus_tag="PLO70_02915"
     CDS             complement(46917..48323)
                     /locus_tag="PLO70_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73433.1"
                     /translation="MLLKKFLPVILLFVVCSAAAQPIKTQPTSRILKTATSLLEAQQF
                     EAAEEYFTKALQNAIATKSSYYQAQAYEGLGNLYSKTSQQARAVEAYQKSIKLYKALG
                     MTVVADVVNSLLKGVQGIGDLYAGVEIGAKGIKLSVIEVKMGKNGTNEYELKTDTSIN
                     TDAAALSYQSEKETHDAITFFYDQIRKRYQIPSSRIHIVISSGLKQELDRYNKVEYFA
                     NVVRPKDLDPNIRIRYITVDEEAELSFKGIVPAINRLNSNQLDVGSGNTKGGYINGNR
                     SFIPVTFPLGTKSFQRLVEAHMTGNPKIEDYLQTAEKIIADSLGRVMVYQFMDKKDFK
                     SKDIYISGGIVWCMASLMHPETIRQNHVELSRQDFADFRKLIFSSYDSLVKPDLRRMM
                     NAEDAKASLANIKRVVNTYDQKALLSGAIWLDELIDQVNTVNPGKKFIFPRYAYVGWI
                     SGYIMDKISKKYTELAVN"
     gene            complement(48627..49604)
                     /locus_tag="PLO70_02920"
     CDS             complement(48627..49604)
                     /locus_tag="PLO70_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQZ73434.1"
                     /translation="MKAFTKTKYGGPELLRLEEVEKPLVKDDHILVKVLANSANPADW
                     HILRGKPFFARFTLGLLKPKDKIPGADFAGVVEEVGKNVQHFKIGDYVFGETLKGGAF
                     AEFVCVPANVCARMPGGTGFPEMASVPVAGLTALQALITHGMLKEGETVLINGASGGV
                     GHFAVQIAKAYGAIVTGVCSGKNADFVKSIGADHVIAYDKENIHQHSEKYDLVIDTNG
                     NLSHKDYKRMGRRGVMIGFTTMGHMMSVLLNKAFSKFPLTQFTAETNSNDLERLAYLI
                     QSGQIKVHIEKTYSYKQIPDAISYIEGMRTRGKVAMLWKNVDNTNRIKT"
     gene            complement(49628..50185)
                     /locus_tag="PLO70_02925"
     CDS             complement(49628..50185)
                     /locus_tag="PLO70_02925"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQZ73435.1"
                     /translation="MNKLLLHTERLLIRNLKLSDLTNFHAYRSDPEVTKYQGFDVFSM
                     MQAEDFINAQADKEFGKAGEWVQYGIENKATGQLIGDCAIKLDGNDTRLAEIGITISP
                     NEQKKGYAKDTLLSILHFLFSLDDFHRVTETVDAENTASIQLLKSVGFRQEGHFIENI
                     FFKGKWGSEYQFAMLKREWLQLNLS"
     gene            complement(50194..51102)
                     /locus_tag="PLO70_02930"
     CDS             complement(50194..51102)
                     /locus_tag="PLO70_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQZ73436.1"
                     /translation="MKIEKQYDVIIIGGSYSGLATGMALGRALRKVLIIDSGNPCNQQ
                     TPHSHNFLTNDGKAPKEIAGIAKKQVQQYDTVTFLDAFVTTGDKTDSGFEIKTDSGES
                     FTAKKLVFATGIKDIIPEIPGFAACWGISVLHCPYCHGYEVRYQKTGILANGDDAYEL
                     ASLISNWTTDLTIYSNGKSNLSEQQRKKLQQHKISIVEKEIELLEHKNGYAERIIFKD
                     GTNAALKAMYARLPFVQHSSVPQSLGCEINVEGYIKTDMAQRTTVPGIFACGDNTTRM
                     RTVANAVAMGTTTGLMLNKELIEQTF"
     gene            complement(51213..51437)
                     /locus_tag="PLO70_02935"
     CDS             complement(51213..51437)
                     /locus_tag="PLO70_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73437.1"
                     /translation="MKTKARLFMSLKIWMVIYPSITLFLFLFGQQLSSLPLYLRTFIL
                     TISLVPWMMFIGVPFLDFLIRRVSKVSTTK"
BASE COUNT        14537 a        10451 c        10762 g        15769 t
ORIGIN      
        1 tacatcatat gacggatagc gatactgtcg ccggctggtg tttcaatcac cacgggcctt
       61 ttcttaatcg cacccacagc catgatggct acctgaggct ggttgataat gggggtaccc
      121 attaaactac cgaaagtacc cacattagtt aaggtgaatg tgccaccttg tgtatcatcc
      181 actttcaatt tgccattcct tgcagcatca gcaaggttat tcacttgttt tgctaagcct
      241 accatattta actggtcggc atttttaata acaggaacga tcaggttacc ggatggcagt
      301 gcagtagcca tcccgatatt gatatctttt ttgataatga tattattgcc atccagggaa
      361 caattgatca acgggaattt tttgatacaa cgtacaatcg cttcaataaa taaaggagtg
      421 aatgtgattt ttgttccttc tcttttttca aattcttttt tcactttatc tctccactgc
      481 accaggttgg tcacatcagc ttccgtaaag ctggttacat gcggactggt ttgcttgctg
      541 cgaaccatgt gatcagcaat caacttccgc atccggtcca tttctattat ttcaacattg
      601 ccggtgtagg tagttaattg ctggctgctg cttgccgatt gatcaggctg cacttttgca
      661 agtgggatca ccatttcgga gcttgtactc ctggcttctg gttgctgacc tccggacttt
      721 cttccaccca catactgcaa aatatctttt ttagtcaccc ttccctcatt tcctgttcct
      781 ggtattgctt ccagttccgt catacttact ccttcgctgg ctgcaatgtt cagtacaagt
      841 ggagaataga aacggttgat gttaccgttt gaagtttgaa gtttgatatt tgatgtttgg
      901 ggacttatag tttcaacaac aacggcttcc tgggcaggca tatttactgt ttcttgcacg
      961 ggagccgaag ctacgggaga agagacaaca ggttcatcgg cgccggttct tatacgggca
     1021 atggtggtgc cgataggaac aacatcattt acattaaaaa gaatttcctc aattacacca
     1081 gcagcagtgc tgggtacttc actatccact ttgtcggtgg caatttccag tatggtttcg
     1141 tccatttgca ccgaatcacc gggcttttta tgccatttaa gtacggtggc ttccatgata
     1201 ctttcaccca gttttggcat tactaaatca acaatggcca taatatttta atttgtttat
     1261 cagcgactga ataaagctca tcacctgttg cgtcgcacac ttcggcttca gaaacacaga
     1321 tgggcaggag acaagcaatt ttaaccggcc aaaggtaatg atttgctata aaaggctatc
     1381 aactgttagc aaggataaat ttgcggagaa agttcaaggc attggtggct gtcataccaa
     1441 tattccgttg ccggtcaaac ctgagattta gctttagggt gtctactttt tctttatttc
     1501 ctgcagctat ccaaacggtg ccgaccggct tatcatctgt tccgccatca ggccccataa
     1561 taccggaagt ggccagtgca aaatcaacat taagtttact gatagctcca ttcaccattt
     1621 ccttcactgt ttcttcactt acggcaccaa cagatagtaa ggtctcctgc ttaactccca
     1681 gtatattttc ttttatttca ttggcatagc taactacact gcctttgtaa tacgccgatg
     1741 aaccggaaac cgatgttatt aaatgggcaa tatacccgcc tgtacagctt tcggcagtac
     1801 ccatcgtttt actttttgct ttcaggatct taccaatcac aatctccagc ccttcatctt
     1861 cgttggtgat gagaaaatct tttaccagtt cctgcaattt ttcaaaataa ggtatcattt
     1921 ccttttccac ttcctctttt ttatcacccc gggaggtcag tcttagtttt accataccat
     1981 aattgggtag gtaggccagt ttgatatgag atgggagaga aggttcaaaa tctttcagta
     2041 gttctgcgat catcgattct ccctgtccag ctgtaaaggc tgttttgtga acaatggccg
     2101 gcattttgaa tgcggataac aagtaaggaa ccacttcatt tactatgaga cctttcattt
     2161 catgcggtac ccccggtaat gatataaaaa ccttgccgtc ccttttaaat agcatgccgg
     2221 gagctgttcc tcttgcattg tgcaatacag tacatacatc cggtacttcg gcctgtttca
     2281 ggtttcgttc cagcattggc ccgggacgac ggaacacttt ctcaaacaaa tagtttacat
     2341 gggccagtac ttcttcattc actaccagtt tgccgccaaa atatttgcaa agtaatggtt
     2401 tggtaatatc atccgcagta ggtccaagcc cccctgtgat gattataata tcagattgac
     2461 gactttcttc atccagagcc tgccatatct cttcatacac atctccaaca gccaacctgc
     2521 gacgaaccca tacaccaatt ttatttagtt cctgcgcaat gaaagcggaa ttggtatcaa
     2581 tcgtctggcc aatcagtaat tcatcgccga tggtgatgat ggaggcgttt acagaattag
     2641 atttcattta agatatgatt agagcgaaat gaagtaattt gaacgcaaag ttataaccac
     2701 agaactatga aaaaagtttt cttactggtc actggctgtt ggatccttgt cactggtgtt
     2761 tacgcacagc tcgtttccca acggttacaa aatgcctttc aacaatttga aagcgatagc
     2821 cagttgcgcc atgccatcag ttcgctctat gtaatcgatg ctaagacagg acaggtggta
     2881 tttgataaaa attctcagat tggtctggca ccggcttcta ctcaaaagat cattacaagt
     2941 gtaacagcgt ttgagttgtt ggcgaaagat tttaggtaca agacagaggt tggaataatt
     3001 ttcggagaga aaagtagctc aatttattta aaaccatcag gagacccaac atttggtagt
     3061 tggcgttgga gtgcaactga ggaagctttt atattagata agttagtaaa taggataaag
     3121 cctcatatta aaagcactga ttcaaatgtc tatgtaaatc aaaaaggatg ggatactcaa
     3181 agcatacctg atgggtggat ttggcaggat attgcaaatt attatggagc tggtgctgga
     3241 gtattgaatt ggcgagaaaa ccaatttgat cttttgctta aatccggatc taaaattggc
     3301 gacttagttg aagtagttaa aacaaatccg gaattatatt cttataagat tcattctaat
     3361 gcgtcgtctg ctaaagcagg ttcgggggat aatgcttatt tatattttcc tgtttctggc
     3421 aacgagggta ttgtgaaagg tacaatacca ataaatgaaa ataagtttca gatttctgga
     3481 gcaatgccat atcctccgat tcaatttatt gaaacacttc gggattcttt atttgggaaa
     3541 aataaacctt acaatcattt atcttctatt tttctttcag aagatccaaa agtaagccat
     3601 aaaatatttt acacccacta ttccccctca cttgattcca ttatctactg gtttaataaa
     3661 aaaagcatca atctgtatgg cgaagcacta ataaaaactt ttgcttacga aaagcagggt
     3721 tttggcagta cggacagcgg tctggctatt gtaaagaaat tctggaaaga aaaaggcatt
     3781 gatgaagatg aactgaatat gtatgatggt tccggtcttt cgccactcaa ccgtgttact
     3841 acccatgcac aggttgaaat tctgaaatac gctacgacaa aagactggtt ccactatttc
     3901 tataattcat tacccttata taacggaatg gttatgaaga gcggcaccat cagcgatgta
     3961 aaaggattct gcggttatca taaagcaaaa gatggaaaag agtatatttt ttctttcctg
     4021 gtgaataatt acaggggcaa aacttctgca gtggtcaaca agatgtacaa agtgctggat
     4081 gcactgaagt agcgagtgat tactagtgaa cggttaatag gaagaaaatc gtaactttca
     4141 gggttaacta taaaccaaaa ctaattgcta tggctgaaca aaattttaaa aatcatgccc
     4201 gcctggttcc aggcttccac ggtgttacct ttttattgat tgtgacaggt cttgtcggca
     4261 gtgttgtcaa tctttttcat gcagatgctg ctacgcatta ttctgctgca ttgctcgtgg
     4321 tagtattttt ggcattgctt ggtgtagcct ggtatgcgag ggtctttgca ttaaaagcac
     4381 aagaccgtgc catccgtgca gaagagaatt tccgtcattt tattctcacc ggcaaaccat
     4441 tggataaaca acttcacatc agccaggtga ttgcacttcg tttcgccagt gatgaggagt
     4501 tctctgcatt ggccaaaaaa gccgcagccg aaaaactttc ccaaaaacaa attaaacaag
     4561 ctgttcagaa ttggaaagca gattattacc gggtataatc agaaacggaa acgatcaaag
     4621 aaactcatat ccatattcag taaccagggt gaggcaatgg ctgccgccca gataaggaag
     4681 atgaatattt ttccggcaac cgacacatcc tgtatcaggt ggcggtgaag cagactttgt
     4741 atcatcgcca ttatcttttc ttctttatgg ctgtattcat acggtgggga tgggtccaca
     4801 tctttgaaag caggatgttc ttcgattaat atattcctga tcttccagta atcttcatcc
     4861 cgcaggtctt catattcaag gtctgtatga ataccagctg cttcgatttt tttttcaata
     4921 gtggtgactt ttttttcacc ggccaggcgc aacaacatca tagcaggaaa taataataac
     4981 ggataaaagg gaagattgat agccagcagg cataagaatg cagctgataa aaaaacaaag
     5041 accttgctcc aaaaactctc ttcatccagg aaaacacggt ttagtaattg gccaccatcc
     5101 aaaggataaa tcggcaaaag gtttatcagg ttgagaacga taaacaataa accgatcctg
     5161 gaaagcgaaa aattcaggaa ataaatgcct gagctatatt ggtcaaccag gaataaaaca
     5221 gcaccgataa taatacccgg taaaggaccc gccagtaaaa taacggctga ctgtaactgt
     5281 gatacatctc ttttacttcc agatacataa gcgccgagca acgggataaa gaaaataccc
     5341 agctcattat accggtaaaa cttcatggca aaaaaatgtc ccagttcatg gatcattaca
     5401 atggcagtga tgagcagcaa aatctggtag ctgggaaaaa tataataccc taaaacgaga
     5461 taagctgcaa ggctgataaa agaacggagc cagatatttg atttctcttc cggtttttga
     5521 tatttaggcg gaatcacaat tacttcttca ggggaagccg ccgtttcatt gcctttcatc
     5581 tcaccgggta gctctgtaaa attctccatc aactaaaatt aagaaaaata agattgaagt
     5641 ttattcaata acagttcagg catgatagtc tttttcatgg tttttgcttc cataaaataa
     5701 aggatgatct taccgactgc cagtaattca ttttgttcat tgaaaacttc gtggtgaaat
     5761 tctattttat gatgtgtggg cagctctttc agcatggttt ttatcgtcag caggtcatca
     5821 tattttgcgg gacgcaggta gcggcaatgc acatccacta ccggcattat cactcccatt
     5881 ttttccatat ctgcatacgt aaaccctaat tcccggatgg attctgcacg ggccgattca
     5941 aaataaggaa agtaattgct atgatatacc acacccatct ggtcagtttc tgcatatcga
     6001 acccttatct tcgtttctgt tgtaaacatt tgacgaagct aaacattatt tcccgatcag
     6061 cttgtccacg ctgattttcc ccgggccgcc caataatatt gccatgtaac caatgaagaa
     6121 aagagccgct ttttcccctt caccgaatat ttctccgtta tgcgacttga acagggccac
     6181 tcccatgact atgatcagtg gaatactggc cagtcttgtc attaaccccg ctacaataag
     6241 aatggcacag aaaaattcag caaaaacaac cattgcaagt gaagtggtgc tgccgatatg
     6301 aaaagggtcc gcaaatctgg cggatttaac tgcaaaattc atcagcttat catagccatg
     6361 cggaatcata aggccaccgg ctgaaagtcg taaaagtagc agagataatg aaatggattt
     6421 atctgaatat ttagtactga agaatgtttt caaagggttt tattttatca aaacgcaata
     6481 ttaaggaata actgcaatgc atcatcttga aaagtgagga gtatccattt tcgtttgatg
     6541 aaacaacaac taaatgttaa agcgttccca acttcttact tatccacacc tgtttggaat
     6601 gatgtttggt ctcacctgaa ataatttgaa tattcgcaac gttaatccac catcggttaa
     6661 aggataaatc aatgaagaat caaagtttac taagtttagc cattttcctc agcgtttccg
     6721 tttttgccca gcagacccgt ttttattcag atcccgaaga aaaatttaaa gaagccaaag
     6781 agtattttca aaaagaacaa tacagcctgg cttatccgtt attcagggaa ctgcgccagt
     6841 cggtaaagga aactgatagg gccaacaccg ctgttacggt acaggaaatt aaatattata
     6901 cgatcgtttg cggcttgaaa cagggcgaag gaaggtcgga agaagaggcg caggagtata
     6961 ttgaggtgga gaagaatgat ccccgggtac aaatgatgaa ttatcacctg gcggaatttt
     7021 atttccgcac tgagaatttt actgaagctg ccaaccggta tgaacaaacc aatattgcga
     7081 acctcagcaa ccgggagata gctgacatga agtttcacca gggctactct tatttcactt
     7141 tgcagcgttt tgcagctgcc aaacccttgt tcaacagcat ccgcagcata aaagatgacc
     7201 cgaattacct ggatgcaaat tattattatg ggtttcttgc ttttcgcgac aggcaataca
     7261 gggaagctct tgaagctttt aaagtggtgg aaaacgaaaa gaactactca acagtggtgc
     7321 cttattatat tgcgcagata tattatgtac agggcagaaa ggatgaggcc atcacctata
     7381 tccagaataa attgcaagcc ggcagcagtt ctcagtacta tgaccttgag ctgaagcaaa
     7441 tgcttggcca tgcttatttt gaaaagaaag agtttgccaa agcgttgcct tacctgtcag
     7501 attatgtaag ccgttctaaa aaagttcgcc gggaagacct gtatgaatta tcttatagtt
     7561 attaccagga aaaccagttg agcaatgcca ttgatgggtt caaacaatta agcggaaaag
     7621 acgattcgct aagccagcat gcgatgtacc tgcttgggga tgcctatctg aaaactggac
     7681 agaaatcaaa tgcccgtaat gcttttttat tttgttcctc caacagcagt taccccgagc
     7741 aaaaggaaat ctccaaatat cagtacgcca agctttctta cgaattaggt taccaggatg
     7801 aagcattgaa cagcctgagt tcttttttag ctgattatcc taattcaaaa tataatacag
     7861 aagcaaaaga gttaatgata gatgtgctgg ctaataccaa taattataag gatgcccagt
     7921 ctttattgga aagtttagca aagccatctg aaaatgcaaa aagattatac ccgagaatat
     7981 tgtatggccg ttcaacggaa ctcatcaacg atggaagact ggcagaagca gatgcattat
     8041 tggataaagc attgaaagat ccgaataatg ggtccgttct tcccttattg aacttctgga
     8101 aaggcgaact ggcctatcgt aataataaaa tcgatgaggc cattaagttt tacaatgctt
     8161 atgttagtgg tggatcacca accagtggtg aagctaaccc tgctaatgcc agttacaacc
     8221 tgggctattg ttatttacga aaagagaatt accccgttgc gctgacattt tttgaaccat
     8281 tgggcagaaa tccatcgctt agttccagtt catttgtaca ggatgcttat atacgtacag
     8341 ctgattgcta tttcatgcag aaagattatg caaaagcaaa aactcattat gataatgtga
     8401 taaaattctc ctggccggca gaagattatg ccaccttcca gattgcaatg ctggcgggag
     8461 taagaagttc aaccgaaaag atctcattat tgaataccat gatgcggaag tttcctgcct
     8521 cttcattggt taccgacgcc aatatggaag ttgccaatac gtatatgggg gatgaacgtt
     8581 tcagggaggc cattccattc cttggcaatg tgataaagaa cagcaataat gccagtttga
     8641 taccacaggc ttatcttaaa ttgggtacat cctattataa catagataat aatgctgaag
     8701 cattaaaaca gttcaaaatt ctggtagata aatatcctaa tgcaccggaa acagaggatg
     8761 ccctggataa tgtaaaaacc atttatatcg aggatggaaa acctggtgaa tatgcaaact
     8821 ttatgcggca ggcaggtcga ccattgagta tgaatacgga ggattcgctt acgtatgcag
     8881 cagcagaaaa ccagtatgat gaccagaata tcaatgcggc attaacggcc ttcaacgact
     8941 acctgcaaaa atttcccgat ggggcatatg cactggatgc aaattttaac cgggcagaga
     9001 tatacaattc aaagaaagac tggaataatg cgttggtggg ttacgatgaa gtggcagtga
     9061 atgcaccaaa taaatttgca gaaagagctg tattaacggc agcaaggatc aatttctttg
     9121 agttaaagaa ttatcagaag gcagaaggct attatggaca attgaagcaa ataactgcca
     9181 gccaggaaaa caagctggaa gcaatgcggg gcctattacg gtgtcaatac cagttgcaaa
     9241 aatggacaga agcagttgac aatgccaagg aattgacttc agcgaaggga agtagtgctg
     9301 atgataaatc actggcgaat atggctatcg ccaaatccta ccaggttgcc ggcgattatg
     9361 gtacagctat tacgaatttt aaatcagtgg tgcagatcaa taaggcagcc ttagctgctg
     9421 aagcccggta tgagatcgcc aattgctatt atcaactgaa taaattaacg gatgccgaga
     9481 aatctgcttt tgaaacgatc aataaatcag gttcatatga ttattgggta acgaaggctt
     9541 acatcctgtt aggagacgtt tatttcaaac agaaggatta tttcaatgcc aaggctacat
     9601 tccagagtat tgtggataat acactgaata cagaattaaa gaatgaggca caggcaaagc
     9661 tgagcaaggt tgtggatgag gaaagtgaat caagtaaggt aggaaactag attttttgca
     9721 gtttaaacag attaaagaaa atgaaacaag cactatataa aatattcttt ctcagtgttg
     9781 ggattttttc tgttgggatt tcatttgcac agaaggatac aactaagagt ggcggtgtaa
     9841 ccattgtatc cagttttaaa ccagtattgc gggaagccgc caaaataaat tttaatgctt
     9901 ctccgcctgc agcagatact acaaaagcaa aactcaatta cgatattccg aaccagaatc
     9961 ttttattggg ctaccagccc ggctccttaa aaccattggc tttatccata gattctgtag
    10021 taaagtttga taacagcagt tatataaaag ccggatttgg aagtctgcgt actccttatg
    10081 tacaggcagg tatcagcttt ggagatggcg aaacggccgg tttaaatata tatgccaaac
    10141 atgtggcatc cgaaagcaaa agggattttc aaaaattcag cagtaccaat gtgcggctgt
    10201 ccggttattt taaatcgggt aataacctgc aatgggatgc cggtttgggt atgaagcggg
    10261 acaggactta taaatatggt tacctgccgg caactttagt tttcccaact gattcattga
    10321 aacaaagttt tcaaaccatc agtggcagga ttgcttttca taatatcaat aaaacagaat
    10381 tcggactgac ttatgcgccg gaaatcaaga ttgatgtatt ttcagataac cttaagaata
    10441 gtgagagtaa tacaattgta aatgcaccgt tgcaaaagac tattggaaaa gaatttgcag
    10501 tgaacctggg aattactttc gatcttaccc gcttctctcc ggaaaataaa acagctgtca
    10561 ataatacgat gtattatatc tcaccatcgg tattgtataa aacatcgcag atcaatgcac
    10621 aggtgggcat ccgtccatcc tgggataacg gcaagtttaa aatgttcccg aacattttgg
    10681 ctgacatcag tacaaccgat aaacgattca ctttccaggc aggatggacc gggtatatcc
    10741 gtaaaacgac ttaccagtac ctggccggac aaaacccctg gttgtggttg ccggggacat
    10801 tacgcaatac ctggatagag gaaagatttg ccggatttaa aggtactgcc ggggatcatt
    10861 ttagttatgc agcgaaagtg ggtttcaata aactgaataa tcaaccattg ttcatcaatg
    10921 atacaagtgc tgcaggtgac gggaaatcgt ttaacgtggt atatgaagat aaaatgaatg
    10981 tggttaattt ttccggtgag tttggctata atgcagggga gaaattctct atgatcaccg
    11041 ggttgatatt taatcaattc tccggctttc agtcacaagc aaaagcatgg gggatgatac
    11101 cgctggaact aaaaaccaat ttaagactgc aaatcataaa agatttgtgg ctgaagactg
    11161 atctgtttgc ctggagtggc tccaaatacc tgaaaaaaga taatacgccg ggtaaacttg
    11221 acggtgcctt tgatctgaat gccggccttg aattcaagat cacaaaaaac atcaatttgt
    11281 ggacgcagtt caataattta ttcaataaag aataccagcg ctggaaccaa tatcctgttt
    11341 acgggttcaa ctttgtcggc ggagtcgtat tttcgttcga ccaaaagaac tgatgatgta
    11401 ctcagaactg tatcaatatc tcattcagca taaacaatta ccggtgccgg gtatcggcac
    11461 ttttttattg gaaagaaaag ctgcaaccat caatttccct gagaaaagga ttgatccacc
    11521 aacttatacc atttctcttc aatcattatt gaacactcca tccaatagtt tttttaactg
    11581 gcttggtgcc gctttgcatg tgtcagacag ggatgcagtt attcgattta atgattttgc
    11641 atttgatctg aaaaaacaaa ttactggtgg tgatattatt aactggagtg gggtaggaca
    11701 attgagcaaa gggttaggtg gtgatgtaaa gttcgttccc ctggattgcc aaaattcact
    11761 ggagacattc gttccggccg ataaagtggt aagggagaaa gccgaacata tggtgagggt
    11821 aggtgaagac gaaaggacat ctgccgagat gactgaatat tttaatcagc cggaagcagc
    11881 aacatcgtat tggtgggctt atgcattggt ggtggcggtt ctttcagtga tatttatagg
    11941 ctggtatttt tccaaacatg gtgttgatat ttcttcagca gctaatggaa agagattgat
    12001 accagcagag acaattgata cccgaaaggt attgccatag tttttcctac ttatttaatc
    12061 attttgattt tactttgctg ctttctgtta tgaaagagct catacattat actcattgcc
    12121 ctgtttgtgg ttctgctgat attgtaaata tcctgtctgc aaaagatcat acggtaagcg
    12181 gtgagacctt tattgtggct ggctgtaatg cctgtagttt tcattttaca caggatgtcc
    12241 cggatgccgc ctctatttct ccctattaca aatcggaaaa ttatatttcc catacaaata
    12301 cttcaaaggg attaatcaat agtctgtatc aatcagtaag gaaaaggact ttgaagcaaa
    12361 aaaggaaact gattgagaga gtggctggta ttaccaaagg aaatctgttg gatgttggaa
    12421 gcggaaccgg tgcttttgcc ggtgagatga agaagaatgg ctggagtgta accggtcttg
    12481 aaccggatga agatgccaga aacgttgcga aacaatcatt tggcgttgac ctgtcggata
    12541 tcaggggctt ttatgaatta ccaaatgaga catttgatgc gatcactctc tggcatgtat
    12601 tggaacatgt gcatgacctg cagggatata tggccaaact gcaactgtta ttgaaagaga
    12661 atgggaaaat attcattgca gttccaaact atacatcgaa ggatgcttct gtctataaag
    12721 aatactgggc tgcttatgat gtaccaaggc atctgtatca tttctccccg caatcgatga
    12781 aggtgctgat agaaaaacac ggaatgaaac tgctccaata caaaccaatg tggtacgaca
    12841 gtttttatgt ttctatgctg agcagtaaat acaagaacgg gaaaacaaac ctggtcgctg
    12901 cttttttcaa tggactgctt tcaaacttcg gggcctcagc tgatgtgaaa agatgcagtt
    12961 cagtgatcta tatcataggt aagtagttat tgaccaaact gtacttcgta taattctggc
    13021 caccatttcc cggtaatata tatttttttg gctgtagcat cgtaagcaat accattgggt
    13081 acttcggcat cggggtcaat ggcttttata cggtcccaca tttgtgtaag atcagctttg
    13141 gctattattt cgccggtgcc gggattgatc ttaaaaatat aaggataagt gtattgattg
    13201 gcatacacat aaccatcaat gtattccagt tcattgaggt taaatgaagg actgccggct
    13261 tctgttactg tctgtgtttt taccagttta aaggaggatg gctcataata aaacaggtca
    13321 ccactgccat tgcttgcaat aaggtttttg ccatctgttg ttaatcccca tccttcaatt
    13381 tctatcggga attccttgat ttttttaaaa tcttttaata aataaacgaa aacagttttt
    13441 tctttccagg ttaactggta aatggtgtca tttaaaatgg tgacaccttc tccaaaaaat
    13501 ttcttgtcaa gagatatttc ctgtaaactc ttcccggttt ttaaatcaac cttttttaat
    13561 ttggattttc cataattgcc tgttccttca tacatcactc cgttatagat cagcaatccc
    13621 tgggtgtaag aagaggtgtc atgcggatat gtggccacta ttgagtaact catacttttg
    13681 ggagcatttg ccggaggagt aacagttcct ggtttgtctt catttttaca ggccacaaaa
    13741 aaagaaagag tggcgataag aaatattttc ttcatttgct tatatataga ttcttacaaa
    13801 agtaagtact ggtaacggtt ttagctgtta aagcttattg aatagcagct gagaataggg
    13861 aaaatacggt atgtacaggt ataggatttc tggattttaa tattatcttt aaacttctaa
    13921 ccttgtccgg cgaaagttta atcccctaaa tcgtatccct gaatcgcctt tttagtattt
    13981 cccaatagtc cctatactgt ttccaatcca aatgaatgac tcatggttgt tatattattg
    14041 ccgtgccctg atgtataaaa tgatttggaa ctatgaaggc gggagtgaac attattttcc
    14101 ttttactggg attaatcagt aatcagctta ttgcacaaat gaattgtgta acttcttcct
    14161 atgcacagga aaagctaaag aacaatcctg cattaaataa tagtataaac gggatagaac
    14221 gatttaccag gcttgtattg gaaaatagct ctgccagtcg cattgaaagc ggtgttatta
    14281 aaatccccgt ggtaattcat aacctttatc atacaccggg agaaaagata tcagatgcgc
    14341 aggtagccag ccagttagaa ttgctgaata aatgttttcg gagaaaaaat gccgactctg
    14401 tcaatacgcc tgcagtattt aaaggattgg ccgccgattg tgaaatagag tttcaattgg
    14461 ctacttcaga tccccgcaaa agagcaacca ccgggattat ccgtaaatac acacctgtat
    14521 caaaatggat aatggatgac aaaatgaaat tctctgctga aatgggagac gatgcctggg
    14581 atagtaaaag ttatctgaat atctgggtat gtaatatgga aaaatttgcc ggttatgcca
    14641 gcatggtggg tggcccggaa gatgttgatg gcatagtaat ggggattgca gcttttggag
    14701 gtacaaataa aacgattgtg catgaaaccg ggcattggct gggactaaag catatatggg
    14761 gcgatgaata ctgcggtgat gacggtgtaa acgatacacc aaagcaggcc agctatacta
    14821 caggctgtcc aactactatc cgggttacct gtggcaatgg tcccactggt gatatgtaca
    14881 ataactacat ggattttacg aatgatgcct gcatgaacct gtttactgaa ggacagaaag
    14941 caaggatgag ggctttgttt gtgggtgggg ctgtccggaa ttcattactt tcttctaaag
    15001 gactggatgc tccattaata tttgaagcac ccttacctga cgaagaccct aaatggctgg
    15061 agccaaaaat gtatccaaac ccggcaacga ccactgtgac ccttgatctt tcttatgata
    15121 ttcgctggat aggtaaaact atttttgtga ctaacctgca tgggcaaagt attatgaatg
    15181 tgatgattac ggcaaaattt cagcaaattg atataagccg cctgcaaccg ggtctttatt
    15241 tccttgcagc aaaaaatgat cagggattgt ctataaaaat gaagtttgta aaactgtaaa
    15301 gtcgcgacca aaatatatac taacggcaag caaagagtcg tctcgtgtaa agcaagacgg
    15361 ctttttttat gtaggtatta tgagcactaa aatgtaaaaa ggattaccag ttgcccggta
    15421 atcctttttt atccttgtac taattaagtt tagatatgaa tagcttctcc ataggccact
    15481 tcaatagcat ctttaatgct ttcgctgatg gtcgggtggg ggtggatcgc atttagtact
    15541 tcatggtaag tggtttctaa tttacgggct gtaacggtct ctgcaattaa ttccgttaca
    15601 ttatatccga tcatatgagt gcccagccat tcaccgtatt tagcatcgaa tattactttg
    15661 ataaatcctt cggtatgacc ggctgcagaa gctttgccac ttgcactcaa tgggaactta
    15721 cctactttaa tttcatagcc ggcatcttta gcttgttttt ccgtatagcc aacagatgcg
    15781 atctcaggca cacagtaagt acagccaggg atgttattat aatcaattgc ttcaggctga
    15841 tgatgatact tcttttcatt gtatgcaatt gcttccacac atacaattcc ttccttgctt
    15901 gcaacgtggg ccagtgcctg tccgggtgag cagtcgccaa tagcataaat accgggcaca
    15961 ttcgattgcc cgtatttgtc aaccatgata cgtcctttat cagttttgat acccaattct
    16021 tctaatccaa taccttcgat attggcagac actcccacag cacttaacaa tacatcagcc
    16081 tccagctcca cttcacctgt tgccgttttt acttttgctt ttaccccatt tcctgaagta
    16141 tcaatagcgg ttacttcact gcttgtcatg ataccaatac cattcttttt gtattgtttc
    16201 tcgagctctt tggagatttc ttcatcttcc acagggacaa tgcggggcaa aaactcaacg
    16261 atagtgactt ttgtacccat actgttgtag aaataagcaa actcaactcc gatagcacca
    16321 ctgcccacca cgatcattga cttaggcatt tgaggtaaca ccattgcttc gcggtatccg
    16381 atgatctttt tcccgtcgat tggcatagta ggtaattgac ggctgcgtcc acccgtggcg
    16441 ataatggtat gcctggcttc aacaatctgt ttctttccgt ctttatcagt tacttccaat
    16501 ttccctttgg aaaccagttt cccaaaaccc atgatcacat cgatcttatt tttcttcatc
    16561 aggaactgta cgcctttgct cattttatca gccacacccc ggcttctttt gataactgct
    16621 tcgaaattgg gtgtaccggc agcctcgata ccaaagtctt tggcatgttt gatatattca
    16681 tttacctggg cgcttttcaa taaggccttg gtagggatac aaccccagtt gagacagacg
    16741 cctccgaggc tttccttttc aataattgct gtttttaaac ctaattgaga ggcccggatc
    16801 gccgctacat aacctccggg accgctacca agaacaacta catcgtatac catattcttt
    16861 atattaaatt gataagttaa taagttgaaa agtaatgagc tgcgaagtta tccgaaaaca
    16921 gtcaattggc aaaagcgggg taatgcggca acccgctgaa caggttgatt aagtcagtgc
    16981 tggtccttca taatagcagc agtgctatgg gatttagctt ttagcacaaa atccatatgt
    17041 ttgtccctga aaatcaatgg ggacatcctt tccacaggct gtgaagaatt ttgccaaaaa
    17101 aacaattaat tccggttgct aattggctga tttcccctac ctttgccgtc ccaaaatttg
    17161 tatcaatcat ggcaagagta tgtcaggtaa caggtaaaac gccgatagga ggaaacaaag
    17221 tttctcactc caatatcaaa acaaaacgtc gttttctgcc caatttgcag aaaaagagat
    17281 tttacctggc cgaagaagat aaatggataa ccttgaaatt atcaacaact gcacttcgta
    17341 ccattaacaa gaatggacta ttaagcgtag taaaagaatt gagagcaaaa ggcgaaaaaa
    17401 tctaacgtat ttaaagttta ataatcatgg caaagaaagg caaccgtgta caagtaatcc
    17461 tggaatgcac tgagcacaaa acaagtggtc agtcaggcac cagccgttat atcacggtaa
    17521 aaaacaaaaa gaacaacccg gagcgtctgg agttgaagaa gttcaatccg attttgaaaa
    17581 aagtaactgt tcataaagaa atcaagtaat catggcaaaa gcagcaaaaa cggcgattaa
    17641 gaaagatcca aaggcagcag cggaggcaaa gaattattct aaagtaatta aggctgttcg
    17701 cagtccaaaa tctggtgcgt acacttttaa agagcagatg gttcacaaag acaaagtgaa
    17761 agattttctg gctcagaaat aattctttga ttctgataag atattcaaaa gctattcgta
    17821 accaacggat ggcttttgtt gtttaaatta gtgagtggta aagaggagac aaaaaaatca
    17881 ggtatggttc tcaattggca gtccattaat agcaaaagtt aagtatggga ttctttaata
    17941 aactattcgg taaaaaggag aaggaaagtc ttgaccaggg tttgcaaaaa accaaggaag
    18001 gatttctgtc taagattacc aaggcaatcg ccggtaaaag tactgtcgat gaggaagtgc
    18061 tggataacct ggaagaggct ttggttggtg cggatgtggg tattgaaacc acagttaaga
    18121 tcatcgaacg aatagaaaaa agggtctcaa aagacaagta tctcaattct tcagaactga
    18181 atagaatgct gcaggaggaa attgagcaaa tgttggtaga tgctcctgaa tcaaatagtt
    18241 acgcttttga ttctgattta ccaaccaggc catatgtgat tttagtagtg ggtgtaaacg
    18301 gtgttggtaa gactaccaca ataggtaaac tggcgtataa ctttaagaag gcggggaagg
    18361 aagttttgtt aggagctgct gatacttttc gagcagccgc tgttgaccaa ttaacaatat
    18421 ggagtgagag agttggcgtg ccgatcgtaa aacaagctat gggaagtgat ccggctgcgg
    18481 tggcatttga tacagtgcag agtgcagtgt ccaggcatac tgatgtggtc ctgattgata
    18541 cggcaggacg tttgcacaat aaagctcacc tgatggagga gctcagcaag atcaaaagag
    18601 tggttcaaaa aacaatacct gatgccccgc atgaagtgtt gttagtgctt gatggttcaa
    18661 ccgggcagaa tgctttggag caagcaaaac attttacggc tgcaactgat gtgactgcgc
    18721 ttgccataac caaactggat ggtaccgcca aaggaggggt agtattggca attgccaacc
    18781 actttaagat accagtgaag ttcattggtg taggcgaaaa aatggaggac ctcttggtat
    18841 ttgataaaca tgaattcgta gatagcttat tcaatttaga aaaatagcag catacaggtg
    18901 atcctgattt ttatgattat aaaagacaga tcataataaa tcatcaccag cataatcatc
    18961 tgcattcttt ttgtatgaag acaaaaaaat tacataagga taaggtcaat atcatcactc
    19021 tggggtgtag caagaacatg gtggacagcg aagtgctgag tggccagtta ctggctaatg
    19081 atattgatgt ggtgcatgag aataagaagc ttgatcataa tatcgtggta gtaaatacct
    19141 gtggctttat tgataaggcg aaagaagaaa gtatcaacac tattctggac caggtagaat
    19201 taaagcgaag aggtaagctg gacaaagtgt atgtgaccgg ttgtttaagc gaacggtaca
    19261 agaataacct ggaagcagag atacctgagg ttgatgccta tttcggtacg atggaacttc
    19321 ctttgatatt aaaacaattt gaagcagatt ataaaacgga actggtaggt gaacgattac
    19381 tggctacgcc acagcattat gcctatatga agatatctga agggtgtaac cgtacctgct
    19441 ctttttgtgc tattccgttg atgagggggc agcacgttag caagcctata gaagaactgg
    19501 taaaagaagc agaaggcctg gtaaagaaag gggttaaaga agtaatgctt attgcccagg
    19561 agctcactta ctacggtctg gatatttata agaaaaggat gttgggggat ctgatgcacc
    19621 gactggcaga tgtgaaagga ctggagtgga tccgtttgca ttatgcgtat ccctctaaat
    19681 ttccattaga agtgttggat gtgatgcggg aaagagagaa tatttgcaac tatctagata
    19741 tgcccttgca gcatgcggcg aacaacatgc tgaaagcgat gaaacgacaa ataacccggg
    19801 aggaaatgga agagttaact atagcaatca ggggaaaagt tccgggtatt tgtttacgta
    19861 ccaccttgat cgccggcttc ccgggagaaa cagaagatga tgtggaagaa ttgaaaggtt
    19921 ttttacaacg tcagcgattt gaccgggtag gtatcttcac atattcgcat gaagaaggta
    19981 catctgcaca tgacctggca gatgatgttc cggcagagga aaaagaaaga agggcgcagg
    20041 agatcatgga agtgcaacag gaaatttctt ttgaactgaa ccaggaaaaa gtgggtaaag
    20101 tttttaaaac acttattgat aaaaaagaag ccggccgtta cttaggccgt acagagtttg
    20161 attcggttga agtggataat gaagtagtaa tacactcagt aaaaaagctt ccgattggtg
    20221 aattcgtaaa tgtaaagatc accaaagcct acgactatga tttggaaggg gaagtggtaa
    20281 atgaggggtc gtgaataact cacaaaagtc ttttcctacc tttatgctgt aattgcttgc
    20341 tatgaagaaa tacatgtctg taggaggtgt tcttttcctt tctgtatttg ctttttcaca
    20401 ggggcaggaa agccaggtta atgcatttga agtacactca aaaggaagcc aggtgttttc
    20461 agttttggcc cttaagaagt ttgctttacc cttacctgaa agggcaataa tttctgaatc
    20521 atataatggt accaggcttt tttctgtttc tgtacacggt tcaaaactaa atggtaattg
    20581 gcaaagttgg tatgcaacag gagtattatg tgattcagga aggttaataa ataacttacc
    20641 ggatggggaa tggaagcatt ggaacgaaaa aggagagttg ctggctatcc gccattacag
    20701 tgctgataaa tttcagcggg taatgcagga aatgctacgg tataacccca aacgaagttt
    20761 cttttactta agtgagctat accagaaaga caaacaagct gccttaaatt atcttactgc
    20821 agcgtattca tttccttcag gcaggtcagc gcctgttcat tctttgaagc aactggtaat
    20881 ctctaatatt tcaaaaagcg atagctacag acctgttttt gaacaatccc ttcaggatgg
    20941 tttgtatatg aatttttttc cgggtggatc catacgagat tccggatatt ataaaaatgg
    21001 agtgagaaca ggaaaatggg tccatcgtga ttctgcggac ggtccctggc acctgggggc
    21061 ttatcagcat agtattaaaa taaaggagtg gaaatactat gataagaata ataaactact
    21121 tgagttgatt gtttacgata acaggggaca tatcaactgg cgaaaaaaaa ttaataggca
    21181 gggaaatcat gaacagcatt agttgtttta gaatccctgc ctgttattct gttaaccgtc
    21241 ctataattaa ttaatcatcg tcatcttttt cctgttcttt aatttcctta ccagaagcat
    21301 ccttttcttt tggattagta ttggttgaat tgccattctg taactcactg tgtcttatca
    21361 atccacccaa atgggcagtt tgtgccataa atccgaatga agcgagagaa aatagtagcg
    21421 ctccggtaaa tagaagtttt gccatttttg tatttttctt taggaatatg gccagaagcg
    21481 aaatggcacc tgttatgctg attataataa gtgatatttt agccatgtcc tcatgttctt
    21541 caatcgcagt ttcattgaca cccggtaaat tttctaccag ttcttcagtt ccttcgccgg
    21601 ttaaaaaaac aggaactgta aaaagtgctg ctgcaataag gaaatacaaa gaaaggtttt
    21661 tgaaagattc attctttttg atcattccaa gaataagtat tccacctccg ataatggaca
    21721 ggaataaagg tgcatggttc aatgcaagat gaatttgtgc tgcattcata attatatatt
    21781 ttaaataaag taattgtaac cggatagtag ttgttaatta ctatcaaaat tgtaaaaagc
    21841 tgtcaggaag cctggggcgg atgaaataca gcataagacc ggctacaggg aactccttcg
    21901 ggatgataaa gtcttattat tcccgcaatt gctgatacta actcttctgt ttcaaaacga
    21961 aatggaatta ttgttagatc aacgtggctg gtcgcttcga aatttttaaa agggagttga
    22021 ttatcctcgc tgtcatcatt atctatcggg tgatcttgcg aataatgaag tgcaagaaaa
    22081 tcaagtaaag aaagcgattt atcctgcttt ttgtgcacct ggaaatgttt caatagcaca
    22141 ggcaatttca gaaactggtg taattcagta gtgctaagta aaaaaaaacc ggttaatata
    22201 gtaaacaggg taagccgcaa aacttgtatt tgtttcaaat tataagtaat atctcagcag
    22261 aaacatgatt gttgtcagat tattgaatta agtatgtcaa aagttagtta atgtttcaat
    22321 ttatccagct actgaaacgc caatgagctt gcctattcca aatgtgatgg cagcagctgc
    22381 cagtccgaat attacctgtc tgaaaccgga gaaccatata ctcttaccgg taaagagtgt
    22441 aatggcagcg ccaattataa ataaaccaac tgcacttgca gctgtactta ataatatcgc
    22501 attgatacca cttaagaaca taaatggaaa aacgggaata atggctccga tagcgaataa
    22561 aaagaaggaa tacaaggcgg cttcaaaagc tgaacctttt aattcttctg catttatacc
    22621 cagttcctct ttcaccaaca actcatgggc atgttccggg tttcttatta tttctgcagc
    22681 catttccttt gcctgttctg caggaatgcc tttggcttta tagatcagtt ccagttcttt
    22741 catctcgttt tcggggtgtg tttccagctc ttccatttct atctgcatct ggttttcata
    22801 tagctcctgt gaacttttta cagaaatcca ttctcctaat gccattgaga gtgcacctgc
    22861 caataagccg gccaggcctg ctaataatac tccttcctgg cctgctgtgg cacccgccac
    22921 tcccattacc aggctaaaat ttgataccaa tccatcattt ccgccgagta cagcggctct
    22981 tagtgcatta ccaccaacag accggtgacg actttcaaaa cgggcaatgg aactgccaga
    23041 aatttctgat tgctctataa tgttgcggag tactttaata tgattgacct ctgtacctgt
    23101 aatgggttgt ttggtttgtt tcttcagatt gattattgaa ttggaaagac ttttctcagt
    23161 gtccatcaaa acccccagta cataatcata accaaatatc tttccaatac gatttaatgt
    23221 tttagctcga aaagagggaa gaggcatttt atccggcgat aagttcaatt ttttaagaaa
    23281 tgcatctgca tggctgcttt caatctcact catttggcga aaaatatctg ctacctcttt
    23341 attttcctca ttatcggcga gttttttata aagaaaagtg gcatctactt ctgtttgaat
    23401 acttctgagg tcaggcattg taattaatta aagaatttat ctaagatatt ctatttaatc
    23461 tgtgctttga gctgatctgc aatttttctt gcttcttcca ttttaccggc tggtactggt
    23521 attcttatta tttcctgctg gtttgcctgc agggtgggaa attcaaatat aatgtgcaat
    23581 accggaattt ttgattcagt ataaatatca actgctatca gatgggcacc caattgtgtc
    23641 catgtgtgga aaatgcctcc aacaaaagcg gaggtattgc cgatatacgc ttcatgaatt
    23701 cctttttgca ggttatagct gcgaatacgg ggagccagaa atgccacaat ggtcaggaga
    23761 agtataatac ctgcaatgat cactatgaaa agattgtctc ggtaaactaa aagaagtaat
    23821 gcacaaacta cgatcgaaat aataatcaca agccgtaacg ttgctttgtt tacggcgatc
    23881 atttctttca ggtcttcttt gatgaagtca tcccattcct ggtcagtata agtccagtga
    23941 gccagtggtc ttaaattgct ggtaagcctt ttaaattccc tggctcttgg tatatatacg
    24001 agtgcagtta caaccgcaga taaggcaaga aatccgaaaa taacaataag tgcataacca
    24061 ccatccattc cctccaatcc actatagccg gtaataaaag ctccggcgat catcaatact
    24121 gcgatgatta taacattcct gaccaccctt tgtggtctgt taatgagctt atttaatgtt
    24181 tctgtattgt ccatggttca gggttattta ttttatggtg atttctccat cccagccaaa
    24241 ctgtacatat tgttttatgc tttcagtagt aagtgtctta ttctgagtgg ttcccctgct
    24301 gctgatcctt attgaaatat tcaatggtaa acctgtaaac tcgaattttc cattactagc
    24361 actattaaca gtttttgtaa ttgtcttact gttatagata taggtaatcg ttacggctgt
    24421 atttggcaaa ggcacacctt caagatcgta agggttgttt tttccagctt cttctttggt
    24481 tttaaaaacc gttccataca aaataccggt gttgttcaca gcggtattgt catttccttg
    24541 ccctgtagtg ggggatggtg aagcagtggt gctttcttct gcaggaagag taatggtaat
    24601 ttttattgtt ttgccggagg cgagatcggt tgtaatagtt ttatgatgac ggttattgta
    24661 tgatgctgaa acacttacca tgccagccgg cacattattt aatgtgaact ctcccttgct
    24721 gtttgcagtt gtattgataa tcccaccggc actaatgctg gcaccaaccg caggttgccc
    24781 atccggccgg taaataattc cggtaatggt accagtatat gcgtatgcag taggattgat
    24841 tattaatctt acaaaatcaa cagcataacc atctcctttt cctgatgtag catcatcaat
    24901 atatattacc agtttgcctg attggatttc gttcataaaa tctgctggta tctttacact
    24961 gataagttta ccaacaggcc cggtctggtt aagactgtta atcactgttt ccaggaacac
    25021 ggcttttcgt ccgttgattg tgaccgtgaa ttttgagccg aatgccgcag cctggaaatc
    25081 gtcaacaaac atctgtaata ctgctgaacg aacggatata ttgctaacag gacataacag
    25141 ggttatcgct tgtggtaaat tctctggtct tgcagtacta tttgaataac catcatagtt
    25201 actcttatta ctggtaccaa ccataatacg gtctgttccc ggaggatctc cgggattgat
    25261 cttccaggga taaccgtgac ttggtgtggc attaccacta aacacatcaa aaccttttgg
    25321 gaaaccaaaa ccgagattat caatatcacc ggcgatgaat attttctcag cggtgacacc
    25381 tccaccggtt tccacttctt caatggtata gccggggtca tttggattgg ttaacggttt
    25441 attggtcttt gaagtccaga tcgtagtaaa accgcaacct tttgactggc tgttgtatga
    25501 ccatgttgac ggatcactgt caattacagt ataggttccg gctggcactt caatattggg
    25561 gagtacagcc cagtaagcat tctgtacacc tccctgacca atcaatccgt atgcctgcca
    25621 ggggccatag atttttcctg aactactgcg taatccaatt gtacctggtt ttttaccacc
    25681 attgaaatag tgataatttt caattctcgt aatgattgtg gccctggaaa aagtaaatag
    25741 ggtaggattg ggcggcccgc ttgatacgcc accgatattg ccattatcaa acactttcac
    25801 ttctgttttt gcatcagcag ggttcactcc tttatctact tcggtggttg gttgtttccc
    25861 ggatttcggc gcccatacag ttgtaaaacc acatcctttt gattcgccgt tttgtgacca
    25921 agtcgaaggg tcactatcaa ttacagtgta tgttcctgca ggcaattgca ttttaggttg
    25981 taccacccat gttgcatttt gcacaccacc ctgcccgata actccatagg cttgccatgg
    26041 cccgtacttc tgcccattac tgctctgcac agcaatggtt cccggtttct taccaccgtt
    26101 atagtagtga tagttttcga tccttgtgat gtaggtggct tttgacagag tgaatacagt
    26161 ggcttttgta ggtttacttg aaacgccgcc gatatttcca ttgtagaaga tcctctcttc
    26221 tacatttgca tctgccgggt caacaccttt ctcttcaggt tttttaccgg taatggcgct
    26281 gtctatcttt cctaatcctt tattgatatc atccaggata tcattaaagc ttttcttttt
    26341 ctttttttga tcagtcttcg ttacatccgg ttgtggagtt tccggtttac tgatacgaac
    26401 aaaatttctt gcaccattgt tattccggct atccgtccat tttcctgtgg cggtatttcc
    26461 atccgcggta attttcagga tatactgacc tgttatgcca ttttttatta tatagttaat
    26521 gaagccggtt tctccaatca ttgtcagtga acccgtagca ttgtcaaatc ctttttccac
    26581 tgccgtatat tgtccattac ctgaggaagt aaaagtgaaa gtttctgtgt tcccatgttt
    26641 ccatacgccg ctgatatcaa ttttgctgta ggatccgtta acctcttttt ccaattctct
    26701 tactttatgg atacgttctc cactattcca ccactcaagg ctttctttca tttttcctaa
    26761 cccgtctttt tttgtttttt tcttactggc tagcttttca ccttcattcc ataattcatc
    26821 cgctttattt ttctttgcct gaatggctgc cagttcagtt tccagttttt tgatcctgtt
    26881 atcaagttcg ggatcaggaa taagtgctac actttcttta tattttccaa gtgcttcttc
    26941 tttttgcccg gcagcttcag ctttctggcc atcttttttc agttgtgcgg ctttatcttt
    27001 attgactgtg atctccttct gtctcttgtc catgcttctg gcaaaatcaa tcatttcttt
    27061 ttgccttgcg tcgttagtac tgaaacggct ggttgctgta gtttcaatag ttgaaggagt
    27121 ttgtttagca tcaatgggta atccctgttc aaattttgat tttgtgataa gaaaatcatc
    27181 atagatcctt ttctgttcag caagttgtgt cttacccagt tgaatagaac tttccacctc
    27241 tttcattgca gccgaagttg gtgtccactc atttttaaat gtttctaacg tttttagtcc
    27301 attttcaaaa tccttttcct tgtactgatc ggaccagttt ttccgggtga tggcatatac
    27361 tgaatcatag cttttcagca aggccacata tttgttgtac ttctgctgtg accatttatt
    27421 agccatctgg ccgggcatat tgaattgcag tttattgagt tcagcaatat ttttttctcc
    27481 ggccggaata ttctttttcg aaatattact ttccagttca tctccttttg ccaatacagc
    27541 aggccattct tttgcccact ttttattttg gtctaattgt gttttggcag cagcatcagt
    27601 aggattcatt tttaatgcct gctcacaacg tttgatagca agattgaaat ctctttcata
    27661 ggcagcgtct ttcgctcctt ttttgcaggc ttctaccaat tcatttatca ggggcgctgc
    27721 ctcttttgca ttcatgcctt ttacttcttc acccagttta atcgcttcat ccaatttccc
    27781 ttcttttaat aattgccttg cctgttgcat tttttcatca aatgcttttt tctggctaac
    27841 cccgttagtg atcatttcat tgctgatggt tgcactgaat acggcaatcg catttcccag
    27901 ttccagtcca ttattattct ttactgttac ttttaattga attgtcccgg tttccagtgc
    27961 ggtgactctt gcatccctgg atgcagggtt actgatggaa gcatttcctt ccgtaacctg
    28021 ccagttataa gtaagattgc cggaaggttg cggagtaaca atagtactga actcgataac
    28081 ctggtgcaca gctatttcct tatccacttc caccaatcca ataccgggtt tccagatcat
    28141 tggtttcggc ccctgtactt ttggaccttc ggtttttact gtataacgtt ctgcattgaa
    28201 gtaagctttt gtattgccca gttcttcgcc gcttccttta acaacagcca atacttcaag
    28261 ggcaatctgc atagcatctt ttgcatagaa agtgatttcg gaaccatcct gtgattcttt
    28321 aataagattg gcattggcgg gaacgggcat ccacctgtaa ctcacgtctt ccagtttgtc
    28381 aggtatgctg ttgatctttg cagtaaccaa ttctccaacc ttagcttttg ccggggtgaa
    28441 tgtaatgttg aatgccggtt tgtctacatc aaattctaac tgatcagatt cacccatggt
    28501 gattgtttct gctgttgttt tatccaatac ctgcacccat acttttttat gacccggttt
    28561 agagaaaata accttagttg tgtttttact tccttcaaag ggatcaaatt tttcttccgg
    28621 gtgtggttgc cataagtatt gaagtttttt acccgcggga attgtactga taaaatcagc
    28681 ttttaattcc accggcatac caacaatgat tttattggta gcaggggaaa cctttgtcag
    28741 ctttactttt agcggggcaa ccacatattc tatcgttgca cttccgggag gagtggtgcc
    28801 gtccacagat actgtaatcg atttttttcc cggcctgtct gataccaact gagcagtact
    28861 gttagtacta ttgccttcca catggccagt ccatgagtag gtatatggac cgtaatcagg
    28921 tttatcattt ttgataacgg ctttcaccga aactttactg cttggttgta tcgtatcaat
    28981 tttgggttca attgtaacca ttggataatc acttccatct aatttaggtc ctgtgtaggg
    29041 agacttcgtg agtcgtccat caggctgaaa tgaagccgat gtcagtatgc tggtagcttc
    29101 cgccgctaat tgttttacca tactcatcat ccatgcctgg tcgctgttat caaatgcacc
    29161 tccggcccct gcataccaat agacttcaaa tatttttttc cctttcatta catagccatt
    29221 aaaacttgat ccggctccgg cagatctgta accggcatca ctccatccgc cccggtcata
    29281 tttgggggcg cttaatagac catagccctt aaagtctcct acggaaatag gttttacttc
    29341 caactgttta taggcctgtt cttttgcctc tttctccata tatttctgga gttcttctaa
    29401 attattgatg gtttccatcg ggtcatcatc tttcagccct acattagcat atgcactcgc
    29461 agtactgatc gcttttttat catttgacca acccggaatc cttggtgcat tcttcatcgg
    29521 ttttcgttcc agcctaaacc aattagcacc tgaaccacct tcccatatat cggtagcttt
    29581 aacagccagg ctcatgtgct ggtattttac ttttttattt acatattgtg agtaaggccg
    29641 tgtacttccg ggttgcaaca ggctgaagga gataccaatt gtaatactgt cctgcagaaa
    29701 ttttttacca ctcttgtcag tagtttcata ggtatgattc acctgtattt tggaacctgc
    29761 acaattttta ttgtcaccaa tagtttccca tccccagctg caatcactga tacgactttt
    29821 tagtagcgcc ggtatatcag cagatacatc aaagatttca tattgtttga tcaatgcagg
    29881 tattttcaac ttaataccct ttgagggatc attaacgtca tacactttta cagtaatgtt
    29941 ccaataccct tttgccaatt ccgttttttt gttattgatg gtacggctca attttgctgt
    30001 gtaggtgaat gttccctgtt ctttggcatc atgggtgtat ttgtttccgt caccaacttt
    30061 aatgtctttt acagtggcaa ggtcataacg gtaccattcg atggttgttc ccgctggcaa
    30121 tggaatagtt tgttgttttt cattcagaat gatcacatca gccgttaccg ggtttgtttc
    30181 tgttgtctcg gtttcgcctt ctactttaag tgtaacatct atggccggac cttcttttaa
    30241 tcttgcctgg taatcctggt aagcttttct gtattcctgc atttttttaa cgatctcacc
    30301 cgtttcatct ataaaacggg gacttcttcc attggcatct gcatcaacca gcctttcgaa
    30361 accaaaacca tattcaccta atattctgag gtgttcttta tcttcttttt cagtttcttt
    30421 gatcggtctt cccagggcag tggccaggtc atatcgtata tcagaagctc tcttagtatg
    30481 tgcttcgaag cattgtatgg ccagctgcaa atcttttccg ggatcggtgg tcagcatcgg
    30541 ggcaccacta agtccaccca tcagtaccag cctcataggg tcctcttgca gcttggatgc
    30601 atccacacca aaaggtctcc atatttcaaa tattttggat tgtattacca gtatctgcct
    30661 gtaaatctgg atatatcttt cgaatgtcgg gatagccact ctttcataag cagtgcctgc
    30721 tgagaattta tctttcaaag cttgtatata taacgggtcg ttttgaattt gctttctgat
    30781 cttcaataat aggctcaggc tgtccatttt tttcatccgc cattcgtcca tgaacaatga
    30841 atcctgtatg cggatgatgg tggcttccag ttcatccacc acacctttct tttcggccct
    30901 tttggttgat tcaatagctg aaaaaacagc agaccataaa cggtcttcaa tcactatttc
    30961 aagactatcc agcattttga tccgctgacc gcttatttca ctggcggcta cttcttctat
    31021 gtttccgttt tctttttctt ctttgaattt ttttactcct accaggtaac ccacaaattc
    31081 attatcaaaa gcaaacagtg tcatcaggtt tttatgtttt gtttcataca tgccaaactg
    31141 atctgttttg cggggtatca ggctttcaaa atattttata tccgggcaat acatgaacgc
    31201 agggtcataa aaaacgtttt tgataccgag tgtcctggca ggttctgatg gtgaaatgtc
    31261 cggatctgtt tccagtgaat ctgctgatcc agttaatcca ttgaatgccc tgggtatttt
    31321 aaatctaacc acatgccctt gcccgtcggt aatagccatt acagccaacc tgtccaggtg
    31381 attacggaaa ttataatcca ttacctggct tactactaac cggtcaatga tattggtgac
    31441 catatatgcc tggccggcaa aagggaacag gtaaaatgta acagccattg cagcaggggc
    31501 agggcttctt gtttgtattg aattataaat acccaatgga atggccagga aaggccattt
    31561 gccctgagcc cagatggtgc ctgctgtcca gctcacattc caggccgatt gcttataatc
    31621 tttgccactc atccacgcat cccatagtgc tatcccatca tccaatttat tcagtatttc
    31681 actggataaa acttttccgg ttttggtttt agccaggtgt tcattaaaac tattaagctt
    31741 attggttaca tgacttgaaa attcgtatgc cgacatggct gttttttgtg catattcagg
    31801 agcatattgc cgccagtttt cagtaaaaac agatttgaac ttgtttagaa ttttctgcac
    31861 cgcttcaggc ccatatcctt tttcaaccag ataatttttt atccgccccc agggatcacc
    31921 aatcaaccgt tcaacaaatg atttatcaag cttgtactcc tcttccatta acttattcag
    31981 ttcttccagc cttgcattca tcatgtcgtg tggaattttt cctgcccgca ggtcaaccag
    32041 gatggtatgt gccctcaaca tggatttggg tatttcagtg ccactttttt catatccttt
    32101 tttaaactcc tcttccattt gtttgaggaa tttatctgct tcctcaaaac gctgatcatc
    32161 attttcgatg gctgcaattg tttttacccg gtatccgaaa gcctggttag catttttcag
    32221 catggctttt ttacactttt gtacaacgtc atccacgatc aggtttacct gttcattgct
    32281 ttccagtttt ttaccggaaa actcttccat caggccggca attcttttag catcaccttt
    32341 attcctgatc acctcttctg tcagggtcat tagtttttgt tcttcaaggg tgagattgaa
    32401 ataaagcatc tggtctttta ctgatacatt tttcaacgct tctttgatca tgaaaaaact
    32461 tctttccgtg tatttgatca ttttaatgat cttctgtccg ccttcaaacg gaccgtgttc
    32521 gatatcatga atagcatgac gaagcatttc gaggctaagc ataggctcca tatctatacg
    32581 taactcaggg tatctttctt ttggatcaaa agaattggcc ggatcatttt tttgtgcctt
    32641 tagttcctgt tccatacctt tttccatgaa cagttgctta ccttcttttt ctaccgtttt
    32701 gatatcaatg atctgcccgt tttgattcct ttccatctcc agtaatttcc atttgccacg
    32761 gcggagcata tccacctcag caaatgcttt tccccattca ccaataaaga cctcggctcc
    32821 tgcaagtcca tgtgcggtgt gtaccacatc gatcgggatc atacccagtc cgctagctct
    32881 tttcaggttc tcgccaaaca ggttgtgtaa ggcctggttg gtggctgcat cggtagcaat
    32941 ggtgctgaag tctatatcag cagcaaagcc taatccatct acattcattt ttacccagga
    33001 tccgatatcc agttgcccgt ataccacacc caccgcttca gggtgattat tgaagtattc
    33061 aatcatggtt tgtttccaga catccatgat catcaatgtc cggcctccca tcagggtccg
    33121 ttcaattcct tcaaattcac ctttccaggc gatcttcggg tctatggggt tatcaccggg
    33181 catggggttt ttatttttga atacttcttt atagcgctga agatcctgta cggttaattt
    33241 gagttccgcc atttcccgga agaacctggt agattgatct ttaagtaatg tttgctgatc
    33301 cgggctcaat acaggttttt gtccggcttc cggaccctgc actttgaaag gcagttgaat
    33361 atgtccgggt acctgcgatt tgtatttatc cagcaacgca gtgattgttg catatttagc
    33421 atcgcccgga tttactgcac caactccgtt gaattttaat ttgaactgaa agggtttggc
    33481 ctctctgccc attttttcga gatcttcctg agtttgtgct gaaatactag tgttggcaaa
    33541 tagtattcct acgaggatgg taataaaccc ctgcctcatg gatggaagag gtaacatatg
    33601 taaagagttt agggtaagtc tgctttttgc aagctattta atatagtatc ttataactgt
    33661 gacagtcatc attatattca atgatttcgg tcagaaggaa tcgttagtta aaagaaatac
    33721 tgttgtcttt tttgaaactg tagtttttga aaaagagtgt actttttctg tacctgccaa
    33781 gagcactaaa atcattatct tagtttcaaa caaccttctt tatggcctca ggtaaatacg
    33841 tttttctcaa cagggacctt agctggctta gttttaatca ccgtgtatta atggaagcgg
    33901 ctgataagac cgttccactg tacagccgaa tctcctttct gtcaattttt tcatccaacc
    33961 tggatgaatt tttccgggta agaatgcccg ctattttcgc ttttagtaac ctggagtcca
    34021 aaaaaatatc cctggaagaa gaatatccaa gggatttggt tcagcaggtt caggcaacgg
    34081 tatttgagca attggaagag tttggcagta tactcaccaa acaattatta cctggattgc
    34141 aacagcagaa tattttcctg tattacaatg aaccggtcag gaaagaacat gcagaaactg
    34201 taagagaata ttttctatcc agggtactgt cttttcttca accggtgttc atacagaagg
    34261 acaaccaggc cggctttttt ctggagaata atgcacttta ttttattatt gacctggtat
    34321 cgcctgagca accggaaaaa catcagtttg ctttgctgaa tataccttcg gccaatttgc
    34381 cccggtttta tgaactacca atgctgggcg atgatcatta tatacttttc cttgatgatg
    34441 tagtaagaga aaacctgcag gaggtatttc ccgggttcga tattcaggag gcgtggagta
    34501 tcaaactgac ccgtgatgct gaaatgaacc ttgaggatga atttattggt gatattgccg
    34561 agaaaattga aaagcagctg gaaaaaaggg agggaggaca tgctacccgg cttttatttg
    34621 atcaacggat gcctgagcca gttaaggatt ttgtacagaa atattttgga ttgagaacgg
    34681 aagaattggt tcccggaggt cgctatcata atcttaagga tctgggaggc ttacctaatc
    34741 ctgtaaaagg gaaaatagca cagatcaact ggcaggcggt cactcatccg ggctttaata
    34801 atcacctgtc aatttttcaa tctatttctg aacgggaaaa attattacac ctgccttatc
    34861 attcttataa ttatattctc cggttcttca atgaagcagc tattgatcct catgtcagtg
    34921 aaatatatat taccctgtac cgggttgctg cagactcgca tatcgtaaat gcattgatca
    34981 gcgctgccag gaacggaaag agagtaacgg tttttgtgga attgaaagcc cgatttgatg
    35041 aagcaaataa tctcaggtgg tcaaagaaga tgaaagcagc cggtatcaaa attatcaata
    35101 gcattccccg gttaaaagtt catgccaaag ttgcgctggt gaaaagatgg gaaaatgaac
    35161 aatggaagga ttattctttt atggcaaccg gtaatttcaa tgaagcaaca ggtcgttttt
    35221 atactgacca tgttttcttc actactaacc tggagtttgg aaaggaactg gatatgctct
    35281 ttacttacct gcaatccaga acccagccgg tactttatgg aaaaatttct tttaatcatt
    35341 tgttagtatc acagtttaat atggtcaagc gtttcaataa attgattgac cgggagatta
    35401 aaaatgctct caaagggaaa cctgcatcaa ttattatcaa actcaataac ctgcaggaga
    35461 gggacatgat cagccgactg tatgaagcca gccaggcagg agttaaagta caattattgg
    35521 ttcgcagtat ttgttgcctc gctgcgggag tggaggggca gagtgaaaac ataactgtcc
    35581 accgtattgt tgaccgttat cttgaacatg caagggtttt tgtttttcat aatgatggag
    35641 agccgctttg ttttatgggt agtgcagact ggatgaacag aaatttgcat agccgcattg
    35701 aagtttgctt tcctgtatac gatgtaaaat taaaacaaga gctgcttgat atcctggacc
    35761 ttcaactcag ggataataca aaggcagttc tatttaccag tgatctcaaa aataacagga
    35821 tcactccggc tgagccacca gtggcagcac aacaatcgat ttatgagtat gttaaaaaaa
    35881 tggcggagta aaaattaaaa ctatctgttt aaaaaaaagc caggcaatca atttgcctgg
    35941 cttttgattt agttaaaact gtattagtgt atcagtactt tccctacagc gattcttttc
    36001 ccgtttctgt ccgttaatac aagctgatag attccttttc catgcttcct gatatccaca
    36061 tccatccgct ggtacggtgc gatgatacta tatgctttat tatatacaat agcgccttta
    36121 gcatcaaaaa ttcttaatgt gtaagaatta gcaccagtac tgtgatatac cacatcaaaa
    36181 tccccattat tcggattagg gagaataaac agttttgcga aagccgaatc tgttatggct
    36241 actgctgctg atgtattact gcagggtaat ccggaagtat tggttacagt tacggtgtaa
    36301 cttcccagtt tatccaggtc aatgcctgtt aatgcagagg aaacagcgcc gctgactgct
    36361 acaccatctt taaaccatgt gtagctatat gttccgggcg gattaatatt ggcagttaat
    36421 gttgtggtga gtccgggata aagccttgta tacggggatg ctgaaatact taccacaggg
    36481 ctggggctta ctgaaaccgc cgttgcagaa gatgatgttg aacatccgct tgcattggtc
    36541 actgtaactg aataactacc tgaattatta acggtaattg attgtgtggt tgcaccattg
    36601 ctccagttcc atgaatttcc ggcggatgcc gttaatacta catttcctcc ctggcagaat
    36661 gctacagctc cgcctgcagt aactgttgca gtgggtaatg ggttaaccgt tacgttaatg
    36721 gcagacgaag tagtggtaca tccgctggtg gtaacagtta cagtatacgt tcctgttgta
    36781 ttgacagtta ttgcttgtgt gctggctccg ttactccagt tataggatgt ccctgcattg
    36841 gcagtgagta ctacatttcc tccctggcag aaagtggttg gtcctcctgc agtagcagaa
    36901 gcctgtgcaa ctgaattaat agttagcgat actgcagttc ttgtagtgct ttcacatcca
    36961 gtggatatat tgacactggc tgcatggtac gtaattgtac cgactatact taatgttggg
    37021 ttggtaactg tgcttccacc ggttgctgca ttataccaaa taatggtatg tcctgcggga
    37081 acagtggctg ttgccgtaag tgttggaaca ggatttccgg gacagaccgt ttgtgattga
    37141 tcaccaccgc tggtgggtgc ggctactgca gtacttccta ctgttattgg taatgtggat
    37201 ggagctgtct ggcaactgcc attgctcgca aaaacactaa ggtttccact ggtgctgcct
    37261 gcattataat ttattgctat ggaagtggtg ccctgtccgc ttgcaatcgt agcgcctgga
    37321 ggagcagccc atgtatatga agtagcaccg gttactgctg cgattgtgta gacaatattg
    37381 gaaggtgtgc cgcaaatatt ctgaaggcct gaaatggctc caacagctcc taaaccaccg
    37441 ccaccattag aaacaacgac gttgatggca ttggatcttt cactttctgc attggtagtt
    37501 gtattctttg accggattgt aaaccacatg ctggcattgg gtgttaaacc ggttgctgta
    37561 taagtattgc ctccaatatt gggtacgaat aaaacaaatt gtccggttgc agcatcaagc
    37621 ctgtaaatat cataaagtgt tgcattgagc gtcgcattcc agttaaaaat aatttcacct
    37681 gcattacaac taacaccact tcctgtaaaa ccggtaacag tgccaagtat tttaaagtta
    37741 gcatcacttg catcactgac tgcaccgctt gaaaccctga tcaatgcggt tgaagtatta
    37801 gtacccgctg gaattgacca gctaagcctc gttgtaccgg caggtacaga agaagatatt
    37861 actgtccagg tactgccacc gttcaaagag tattcaactg tttgtgtgga tgtaacaccg
    37921 gcattgtccc aggtgatggt ttcgttggta ccgggattga atgattcgcc tccatttgga
    37981 taaatcactt cgatgtatgg ctgatcaaca ctccatgtca atgcatagtc ttgtgaaggg
    38041 ccggtaggta ccgcagtacc attcactctt aaagtatatg atcctgccgc cggattgtta
    38101 atggttacct gttcaatatt actcacatta tctactccct gcgtagcagg agtggccggg
    38161 ttattgggat ccaatatcca gggtaaagtg gttgttgctc cattaacaac agtcagatca
    38221 agattattca ccaaagcagg gtttgcatta gcagctccgg ccgggtcatt ccaggtaagc
    38281 attactctta acctggctgc acctgcgggt acattaataa ttatatcatt tgatgtgcca
    38341 gttgtaacat tatttaatgc ataacggttt tcttctaaaa ttctaactgc ttgtaatgca
    38401 ttaagacggc cataaccgaa cctgtaatcc ggcccggcat tacccaggtc atgtgcagta
    38461 ttaagaacag tattttttat taatgtagat ggaggaaggg tattgctgtt taattgtttg
    38521 taccgttgta ccaataatgc caaagaaccg gcaatacctg gtgtagccat tgatgtaccg
    38581 gataaagtgg catagccatt taccggtgtg gaggtagaaa atacactggt gccgaaggca
    38641 ctaatttctg gctttaccct cccgtcctgt accggtccgc aactactact tcctgataaa
    38701 gcttccaatg ttgtgatgtt ggcaacaagg atattatttt ttgcagattt accactggcg
    38761 gtgatagtgg accatccccc ggtgcaggct gtctgtgagt taccggccga gtgggcatgc
    38821 aggtgataag ggaaattatt aagattcaaa tcagtattac ggctggtagc ggaataagta
    38881 acgcctgctc cattcaatcc gcaggtctgt gtacttccat aggagtggct gcttataact
    38941 aagccttgtg ccggaatagc attagccatt tctgtttgaa tattaccact gaaattaaaa
    39001 gaatacaacg atgcattggg agccatacct cttgcagtct gattaatcag tccgcggcca
    39061 agaatagttc cggcacagtg tgtggagtgt tgtgaagggg aactgaattc catctggttc
    39121 aagcgtcctg atggtgaaaa atccaggtgg ggacttattt caccttcgtc ccagatacca
    39181 atattgatgc catcgccttt caggtttctt actccgtcat tcaatacatt aacccgatgt
    39241 aatgtccttc ccaatgtatt ctctaactgg tttggtgcat caataaattc aacccattga
    39301 acgtatggga gatttatcaa ttgctgcata ttcgattgcg gtatacgtat ggtgaaactt
    39361 cggaacatcg gcatgtcttc cagtatagaa gcactaagcg gcaatagtga acccgttatc
    39421 tgcccgacag ttaatttttc ataggtaata acggtcaggt caacataacc gggctgtttg
    39481 attgcatgag gtggtattgt tccactcaat agagccggaa cagttttttg tgcagcggtg
    39541 aactgaaata cactgcggag gttaaatgat ttaaaactac tgagatcgaa atcagctgat
    39601 acactaaccg tgtatgcata attaggtata taatcgatca gctctatccc tgcagctttc
    39661 aggttattct tttcctgttg ggcaggcaat gctttgaact gtattgtaac ataatgcttg
    39721 ttcagaaaca agctttgccg gaattcggct gagttttttg taagaccact gatattttca
    39781 gcaggaataa atttgccgga gttcagcaga atagtgtagt catttttttc ctgggcatgc
    39841 agtatgccgg caaaaatgat gaaaagcaat aaatgtgtaa gacgggtaaa aaatcgactc
    39901 atagtttgtt ttttagcatg ggtgcaatga aattgtctgg aaaaataagg attaattttt
    39961 gtccgtggtg cgactgtaca acacaatcaa acgattgatc aggtcgctgt tgataaaatt
    40021 ctgaaagcct tacctttaac cacttaaaca atattatatg tcatttgaat ggaaaggtat
    40081 ttttcctgcc ctgttgaccc cgtttacaaa ggatgatgaa cttgatatcc ccatgtttga
    40141 aaaaaacctg caggcgcaac tggaggcagt ggttcatggt atcatcattg ggggttcttt
    40201 aggagaagcc agtacgatca ctgaaaaaga aaaagaagaa ttgacggagt tttcagttga
    40261 gtacctgaac ggtaaaattc ctgttattgt aaacattgca gagagcacta caaaagaagc
    40321 agtaaggcag gcggcgaatg ccaaaaaatg gggcgcagat ggtttaatgc ttttaccacc
    40381 gatgcgctat aaaacggatg acagggaaac tatcacctat tttaaggcag tggcaagatc
    40441 cactgaactg cctgttatga tctataataa cccggtggat tataaaattg acacaagacc
    40501 tgagcttttt gatgaattag ttgactgtgt aaatattact gcattgaaag aaagctcaag
    40561 agatgttacc aaccttaccc ggatgaaaaa tcgatttggt gaccggttta agattttgtg
    40621 cggggtggac accctggcga tggaagaact ttgtcttggt gcggatggct gggtggcggg
    40681 attggtttgt gcttttccaa atgaaacagc agcaatttat aacctggtaa aggccggtaa
    40741 gattgaagat gcaataaata tctatcgctg gttcatgcca ttgcttgagc tggacataca
    40801 tcccaaactg gtacaatata ttaaactggc cgcctctcaa accggcatca gcaatgaata
    40861 tgtaagagca ccgagattag tattggaagg agaggaaaga gaaagaatat tgaaagtgat
    40921 caatgaggga attgccaaaa ggccgagact ggatatttga ttcttgttgt agtcggttta
    40981 ataacagcat tcaatatgac ttcctcatca taaaatgaaa atatgtacaa agacactaca
    41041 atagaagaaa ttaattccgt gatggaacag gcatggaaag cttttcattt gtacagaaaa
    41101 ttatcgctga agcaacgggc cggttttatg cgggccattg catttgaact ggaagaatgt
    41161 ggcgacacat tgatacaaac tgccatgcag gaaacaaacc tgccggaagc aagactgaga
    41221 ggagaaagag caagaacgat ctatcagctg gatagttacg cattggcttg tgaaaaaggt
    41281 gactggctcg aagcaagaat tgatacagct atccctgata aaaatccacc taaaccagat
    41341 attagaaaaa tgctggtgcc actgggacca gtagttgttt ttggagccag caattttcct
    41401 tttgcttatt ccactgccgg gggagataca gccagtgcct ttgctgccgg atgtcctgtc
    41461 attgtaaaag cacatcctgc ccatccgcaa acatcacaga tcgttgcgga tgctattctg
    41521 aatgcggctg taaaaagcaa aatgcctgat gggatatttg ctcatattca tggaattgct
    41581 aatgaagttg gaaaagcgct ggtaacgcat acctatacaa aggcagtagg ctttaccggt
    41641 tcttatgcag gaggtaaaca gttatttgac tgggcaaatc aacgaaaaga acccataccg
    41701 gtgtttgcag aaatgggaag tgtcaatccc gttttcttac tcccggaaaa attacagtca
    41761 tctgctgacg aaattgcaac gatgtatgcc ggctccatta cattgggagt gggacagttt
    41821 tgcactaacc cgggcctgat cattggtata gaaagtaaag ccctccaaac ttttattcat
    41881 gacctgggaa aagcgattca gcaaattgtt cccggtacta tgttgcatcc cggcattgcc
    41941 aatgcatttg ctgcaaagaa agaagaatcg ttactgcaac cagatgtcca tctcgttgcg
    42001 gaatctgcaa tagctccaac ggaaaaacaa ggacaaccca ctatcgctac tgtggatggg
    42061 agaacatttt tatccaatcc tgtcctgcac aaggaagtgt tcggacctta ttcattggtg
    42121 gtgcgttgtg cggatatgga ggaaatgatt gaagtagcaa aacacctgga aggtcagctg
    42181 acttccacta ttatggctac tacggcagat ataaataata atgatgagct ggtagaagcc
    42241 gtgaaaaata tttgcggccg ttttattcta aatagtgtac caacaggggt agaggtttgt
    42301 ttaagtatgc accatggcgg cccatttcct gccacaactg atgcaaggtt tacgtcagta
    42361 ggtgcagacg ccatcaaacg ttttgcaaga cccatcgctt ttcaaaactg gcctgatgaa
    42421 ttattacctg atgaattaaa aaatgccaat ccaattggaa tttggagaat ggtgaatact
    42481 gaaccaggga atacagctct ctgaattaac ttaagactat tgtttctgtt catctttttt
    42541 aatcgttgaa accttttcag tacgttttaa ttcaaaaatg aaatgactga acggatcgct
    42601 gtaatggaaa ttaccttcgc aaatagctgt tgaagccggg ggattacttt ttatttcacc
    42661 aatgatctca tctgctgtaa caaaactttt attcattgct ttattaaaaa gtacattcag
    42721 gaaacttccg gcaaaagggg agtgctgccc cggtttacca tctgccacat attgtttacc
    42781 gccggatgaa atgcctttat aaaaccgttt gtttgatgca ttcttcatga gagttgacag
    42841 gttttttgca tccacatccg gagtgccacg tactgtattg ttatcaaaga aggaacctgc
    42901 aaaacaggca tccaggacca ggaagatccg gttgcaactg atatttttca ggtagatatt
    42961 gccaaggtcg ctataagaaa gataggtctt atgtgtctgg tcatctaccc tggaatcttt
    43021 ggcaacgaga taacccatct tcgctttatc ataatacacg ccatgcccgg caaaaaagat
    43081 cagcaattga tcattgggcc catagctttt attatcccgg tactcactca accatttctc
    43141 aatgtcatct tttgatgggt ttttcaccag tttcacttca aaaccaaatt catttattaa
    43201 taatgattca agcgcagttg catcattgat ggggttaaca agtttattaa atccggcatc
    43261 catgtaattg tctgtagcaa agagcagcgc ataatttttc tgacttcccg ttacggcggg
    43321 atcattatta ctggtggcgt tttctttcac gatcagtccc ctggtgccag ccatattaat
    43381 ggtggcggca ctgtcatttt tttcattttc aaacaggaca aaatctttgc tgttggtaaa
    43441 tgacagtaca ggtaaataat gttcgccccg cccgattgca aaggaggctt tatcaataaa
    43501 atctttagca gtgaaactgc ctttctcttt caggttattg aggctgagtg ccagctggtt
    43561 tgtaaacagc gaaacgccgt tttcctgcac cggtgtttca atacccgggc tcagaaaaat
    43621 tatttcagct gaattattac cggtattggc atatttggtg gtgatcaggt tcagtaacga
    43681 agggttgggc aggtcagtta ttaccatttt cttattggca tataccaggt caatatggtc
    43741 catcagttcc gtgatggcaa ttcttcctga agtagtatcc ttcgtattga aatcaaggta
    43801 atgctgacca ttgctgctgt tgttctcccc tgaataataa aaaacaaaaa tatcttccgg
    43861 ctttgagttt attctcacca ggtcaataaa gccggtaagt ttttcgcggt tgatctctga
    43921 tccgtttacc agtttattga aacttttttg attctccttg tcttttatcc cggagatact
    43981 gtgaaaaaag gcgcctgcat tatttttgcc ggagtattca cccggccggc tggaaccgtt
    44041 aagggcaatg atatgcaggg caggggtggc atatagttct gttacttttt gtaattcggc
    44101 attgtacaat attttgtatt gctcagcttt tgttttattc ccggtgttgg tataaaaatt
    44161 atagatcctg tcataatcac tcacggtata atcatcctct cccgtagtat atatattccg
    44221 ctgcaggctg agtttttccc gggctatttt cagcgccgcc tggtaatgat acaatgcgct
    44281 gtcatacata cgggcatagt aataggatac gcctatccag ttatgtgcat tgtgaatgga
    44341 actgacgggt ggttctttgc tcttgtcata cagtgtaata tattctttcc agagtgatgt
    44401 tgcagctttg taatttttct catcatattg ttgccttgct ttcttccagg tttcattacc
    44461 ttttgtgatc gcataatatt tttcttcaaa ctctttgtcc atccagtcgg ccatttctgc
    44521 cactgctttg cggttccagc ctttcttaaa taaaaaatca aaatcagttt tagggccggt
    44581 ggtataatca gccggggaat atagattatc cagtgttttc tggtaataat accgggcact
    44641 gtcggtattg ccctttatca ggaaagtatg tgcatagttt acggtgaaag ccacattggt
    44701 ggtatctcca aaataagcag ttctgcaaaa gggtattgct tcatctacct tgccggcata
    44761 caatttatac cagccgtagt taccggtaat attgatattg cctggtaact ctttaagcag
    44821 ttcatcatac aattgaaaac acttgtcaaa ttgttcagct tttccatata aactggcaag
    44881 ctggtaccgg tagttaatat gttttggatt atacgaatat gctttcaata catcagcaag
    44941 tgctgctttg gagtcaccat tggcttctgc caggaaagac cgggtatagt aaaattcatg
    45001 ttctgcaact gtatttttta atgcttttgc aacaaagatc aatttgttgc aaagttgtaa
    45061 agccgcttca tttttttctt cagtttgtaa tgcattcact ctttctgtcc attcaataag
    45121 agagtcactt tgtatataag cagccgattt ttttaccagg tcacccagct gatctatgtt
    45181 tgttttttct atgagtggca gcagccattc ccggtttctt tctccctgtg gtccgtagtt
    45241 gatcacccag gtggcaaatg tttcattgat cttccattca ttacccatgt acttgccggg
    45301 atattttctc acaaaatgaa aaaagctgtt caggtcataa taagaagctg ttttgaacat
    45361 atccaccagg ttcctgcctt taaaggggcc tttggtatgc ggaactttta atgaagagtc
    45421 gatatattgt tctacatcat ctttaaaatc cagtatttca tcagtataca attgcatgaa
    45481 gtccgtttca aaatccgggt tcgtattcct gatagcatct gctatagtaa aaatgggctg
    45541 acggttatta tccaaaaaaa gaatattcag tttagccagc tcataaaaga tattcttttt
    45601 gatccccggc attgtataag cagataactc caccaggtcg ccgccataaa ttttttcatc
    45661 caggaattta ttgtaaacgc ttatcttaac agctgcactg ctatctgtga tagcgatcac
    45721 ttcagcatta ccgaggtaga tagcagtttc ccgggtattg ttattatttt tttcattggc
    45781 caccctcgtt gtaaaaatag taccggcagc acccttaaaa agcccatcct tttttccata
    45841 gcttatatag gcaatggcag aatcaggttt aacccggttt actcttttta tctcaaaaaa
    45901 gaaattttca gttttagtgc tatcaaattt attgaaagga gttttggaga cgggcctcat
    45961 ttgtccttgt acctgtccgt acaacagtat ggtgaataga aaaacaaaaa gacgctttgc
    46021 catggccatg gtgctaaaat taattgctga aaattaacta ttaatttctg tatcaacgta
    46081 cccgtctcag ggtatttgat agaggccggt aaccggtgct gcaggtgtta taaggtattg
    46141 tgttggtaaa cacgatttaa ttatgttaca ttcgccgcca aatacgaaag tggaaacagg
    46201 acaaacagta ttgaaatata tttaagaacg acctggctct cttttcaggg agttgaaggg
    46261 acaaaaacga ctgactaatg aaacaagacg aattactggc caaaagaagc ggtaacaaat
    46321 gcgaattatg tgaagcaaca gaacacctga aagtatatga agtgccaccg gctacaggta
    46381 atatggatga tgcgatactg ctatgcccta aatgcctggc acaagtagaa aaaaaagaag
    46441 aactggacag ctcacactgg cagtgtttaa ccaccagtat gtggagtgaa gtaccggccg
    46501 tacaggtaat ggcctggcgt atgcttaacc gtttacgcaa tgaaagctgg gcgtcggaac
    46561 aactggatat gctatacctc gatgatgaaa aactggcctg ggccaaagcc agtggcgacc
    46621 atgagaacga tgcttcagtt gacctgcata aagattgttt tggtgctatt ctgcaaagcg
    46681 gggataatgt ggtactcacc aaatcactgg atgtaaaagg ctcttcggtg aatgcaaaag
    46741 ttggaactgt tgtacgtaat atacgtctgg tgcctggtaa tacagaacag atagaaggga
    46801 aaatagaagg tcagcaaatc gtgatactga caaaatattt aagaaagcag ggataagaaa
    46861 atagccattg tctgaccggg taaacaatgg ctaaataata agtatttatc cggtaatcag
    46921 tttacagcaa gttcagtata ttttttactg atcttatcca tgatataccc gctaatccag
    46981 ccaacataag catacctggg aaaaatgaat ttcttaccgg ggttcaccgt atttacctga
    47041 tctataagct catccagcca gatagcgccg gagagtaaag ctttctggtc ataggtattg
    47101 accactcttt taatattggc cagcgaagct ttggcatctt ctgcattcat cattctccgc
    47161 agatcgggtt ttaccaggct atcgtaactg gaaaaaatca atttgcggaa atcagcgaaa
    47221 tcctggcggc tcaattccac atgattttgc ctgatagttt ccggatgcat cagggaggcc
    47281 atgcaccata caataccacc ggatatgtag atatcctttg atttaaaatc ttttttatcc
    47341 atgaactggt aaaccattac cctgccgagg ctgtcggcaa tgatcttttc agccgtctgc
    47401 aggtaatctt cgattttggg gttgccggtc atatgggctt ctactaaacg ctgaaaagat
    47461 ttggtaccca gtgggaaagt gacgggtata aaagaacggt tgccgtttat atatccgcct
    47521 tttgtattac cactgcccac atcaagctgg ttagaattga ggcggttgat ggcaggtaca
    47581 attcctttaa aactaagttc ggcttcctcg tccacagtta tataacgtat cctgatgttt
    47641 gggtcaaggt ctttgggtct taccacattg gcaaagtatt ccactttatt atatctgtcc
    47701 agttcttgtt tcaggccgct gctgataaca atatgtatcc gtgaagatgg gatctggtat
    47761 cttttccgga tctggtcata gaaaaaagta atggcgtcat gggtttcttt ttcactctgg
    47821 taagataaag ccgctgcatc agtattgata gaggtatccg ttttcagttc atattcattg
    47881 gtcccgtttt tccccatctt tacttcaatc acacttagtt taattccctt tgcacctatt
    47941 tccacaccgg catacagatc gccaatgccc tgaacccctt tgagcaggct gttcactaca
    48001 tcggcaacta cagtcattcc cagtgccttg tataatttta ttgatttctg gtaagcctct
    48061 acagcacggg cctgctgact ggtcttgcta tacaggttgc cgagtccttc ataagcctgt
    48121 gcctggtagt aactgctctt tgttgcaata gcattttgca gggctttggt aaaatattct
    48181 tctgccgctt caaattgctg ggcttccagc agggaggttg ccgttttcag aatgcgggaa
    48241 gttggctgcg ttttgatagg ttgtgcggca gcactgcaaa ccacgaataa caaaataacg
    48301 ggtaagaatt ttttcagtaa catgttcata tagttttata gtcaatattg aaacagcata
    48361 aatccgcaac agctgttgcc agaacaaaag taaaatattc acctgcgggc attggcagtg
    48421 gcggtggcca attgtgaaca ggttttcaac atatttcgat ttactgctga aggaaataaa
    48481 aacgctttgt agaacgatga atgcaatacc ggctccaaca gaattcattg gcttctgtat
    48541 cagaacaaga ttgtggataa atttgataaa gcgattgaag gcagggtaga aaaaagtatt
    48601 tttaaaactc acagaatcac ttcaattcat gtctttatcc tgtttgtatt gtcaacattt
    48661 ttccacagca ttgctacttt gcccctggtc cgcataccct caatataact tatggcatca
    48721 gggatttgct tataagagta tgttttttct atatgaacct tgatttgtcc actttgaatt
    48781 aaataagcca atctttcaag gtcatttgaa ttagtctctg cagtgaattg tgtaagcgga
    48841 aatttactga aggctttatt aagtaatacg ctcatcatgt gacccatagt ggtgaaacca
    48901 atcatcacac cccttcggcc catccgtttg tagtctttat gcgaaagatt accattcgtg
    48961 tcaatgacga ggtcatattt ttcactgtgc tgatgaatat tctctttgtc ataggcaata
    49021 acatgatctg ctcctatcga tttaacaaag tcggcattct tgcctgagca aacaccggtg
    49081 actattgcac catacgcttt tgcgatttga actgcaaagt gaccgacacc acctgaagcg
    49141 ccattgatca atactgtttc tccttctttg agcataccat gtgtgataag tgcctgcaat
    49201 gcagtaagac cagctaccgg tacactggcc atttccggga aacctgtccc accgggcatc
    49261 ctggcacaaa catttgcggg gacacaaaca aattctgcaa aagctccgcc cttaagcgtt
    49321 tcaccaaata catagtcgcc aattttgaaa tgttgtacat tctttccaac ttcttcaaca
    49381 actccggcaa aatcagcacc gggtattttg tctttgggtt tcaataaacc aagggtgaac
    49441 ctggcaaaga aaggcttccc ccggagtata tgccagtcgg caggattggc agaattggca
    49501 aggactttta ccaggatatg atcatctttt accagaggct tttctacttc ttccagtctc
    49561 agcagttccg ggccaccgta tttagttttt gtaaatgctt tcattttcct ttaatgaagt
    49621 atgctattta agaaaggttt aattgtaacc attccctttt cagcatggca aactggtatt
    49681 cgcttcccca tttgcctttg aaaaaaatat tctcaatgaa atggccctcc tgcctgaacc
    49741 cgacactttt cagcaattgg atagaagcag tgttttctgc atctaccgtt tctgtaaccc
    49801 tatggaaatc gtcaaggctg aataaaaaat gcaggataga aagaagagta tctttcgcat
    49861 aacctttctt ttgctcgttg ggtgaaatag taatgccgat ctcagcaagc cgggtatcat
    49921 tcccgtcaag tttgatagcg cagtcaccaa ttaattgacc ggttgctttg ttttcaatac
    49981 catactgcac ccattcaccg gccttcccaa attctttatc tgcctgtgcg ttgatgaaat
    50041 cctcagcctg catcattgaa aagacatcaa agccctggta tttggttact tccgggtcag
    50101 agcggtatgc atgaaaattg gtcaggtctg ataatttcag gttcctgatc aaaagtcgtt
    50161 ccgtatgcaa tagcaatttg ttcatttgcc tgtttaaaaa gtttgctcaa tcaattcttt
    50221 attcaacatc agtcctgttg tggtacccat tgccaccgca ttggctaccg ttctcatccg
    50281 ggtagtattg tcaccgcagg caaagatacc cggaacggta gtcctttgtg ccatatctgt
    50341 tttaatatag ccttccacgt ttatttcaca acccagtgac tgcggaacag atgaatgctg
    50401 tacaaaagga agccttgcat acattgcttt cagggctgca tttgttccgt ctttgaaaat
    50461 gattctttca gcataaccgt ttttgtgctc caatagctca atttcttttt ctacgataga
    50521 gatcttatgc tgttgaagct tttttctttg ctgttctgaa agatttgatt ttccattgct
    50581 gtaaatcgtc aggtcagttg tccagttgga aataagtgat gccagttcat atgcatcatc
    50641 cccattggcc aggatgccgg tcttttggta cctgacttca tagccatgac aataagggca
    50701 gtgcagcaca cttatacccc agcatgccgc aaaacctggt atttcaggta tgatgtcttt
    50761 aatgcctgtg gcaaacacca gttttttagc tgtgaaactt tcaccagagt ctgtctttat
    50821 ttcaaaaccg gaatcggtct tgtccccggt tgttacaaaa gcatcaagaa atgtaaccgt
    50881 atcatattgc tgcacctgct tttttgcaat gccggcaatt tctttcggag cctttccatc
    50941 gttggtcaga aaattgtggg agtggggagt ttgttgatta caaggattac cgctgtctat
    51001 gatcaatact ttccttaatg ccctgcccag tgccatgccc gttgccaaac ctgaatagct
    51061 accaccaatt attattacat catattgttt ttcgattttc atcctgctgt gtgttgaatt
    51121 gccaaagaat gggagcggga cgatcatccc gctgaaaatg atacctgttt gtttaatgaa
    51181 gtcacgcctt tgtaccataa agaatgactg cattattttg ttgtactgac ttttgaaacc
    51241 cttcgaataa gaaaatcaag gaacggtaca ccgatgaaca tcatccacgg cactaatgag
    51301 atggtgagga tgaatgttcg caggtataaa ggcagggaag acaactgttg cccaaataga
    51361 aaaagaaaaa gggtaatgga aggataaatt accatccaga tcttcagtga cataaaaagt
    51421 cttgcttttg ttttcatgtg taaatttttg ttttaacaat ttgtcacatg ttattcgcca
    51481 taaaattttc atcgccggtt ggtataatag ctcgaaaat
//
LOCUS       DAORTI010000011        49824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_389_length_49824_cov_6.713979, whole genome shotgun sequence.
ACCESSION   DAORTI010000011 DAORTI010000000
VERSION     DAORTI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 49824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49824
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_49824_cov_6.713979"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLO70_02940"
     CDS             <1..337
                     /locus_tag="PLO70_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQZ73438.1"
                     /translation="ELVNEEAVKKCEPDFSAMKKYFDKMIITAPGKEVDFVSRFFAPG
                     AGIDEDPVTGSAHSQLIPFWSEKLGKNKMLAKQLSQRGGDIYCEQLNTERVIMGGECV
                     FYMKGVISI"
     gene            complement(342..1109)
                     /locus_tag="PLO70_02945"
     CDS             complement(342..1109)
                     /locus_tag="PLO70_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ73439.1"
                     /translation="MSKIKTLLVDDEPRGQTSLHKLLQLNCPEVVVIGYAGNATEAKE
                     KIEELKPGLIFLDIAMPERNGFDLLKSLPVIDFEIIFVTAHENYMLQAFHFSAIDYLL
                     KPIEDDLLTEAVKRAGKRIEEKSGGQQLETFLHNIHHKGPRQKMKLCIPSLKGFQVVE
                     IKDIIHCEASSNYTNFHFTNRVSICASKPLHEYEELLADCNFVRVHKSFVVNLEHVKE
                     YIRGEGGSVLLSNNLEVEVSRRKKDMLITKMKEYYKF"
     gene            complement(1106..4237)
                     /locus_tag="PLO70_02950"
     CDS             complement(1106..4237)
                     /locus_tag="PLO70_02950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ73440.1"
                     /translation="MQPNFPYKTFLCLLSFLTSIIQPCLAQYEESDFVRYTVKDGLSD
                     NYITCLQQDNLGYIWAGTNNGLNRFDGHSFNNFYQGSPDLPLASIDIRNIKIIGPQQL
                     GIISGGGLEIIDTRDFSLKRFYIPDSTAFSTYQNAAWDAVELADHSYAITTASGFYVF
                     NPSGTIKFRHDAYTLPDIGKKRILYGHEIFPLNKSEQVVYFEESGMAIYNAEQRSYRE
                     PGTEEIKYAALAHPSITMGGQWITKYQLNNDEFFFIFLRKDSIIYYNHQLQKVITSPV
                     PFPTSSAFSWESRITKLNDSSFMINDRTRGFYIFNLDRKTGQIYFNKKKFLSSYKIVR
                     LFVDKDNRLWAGTSGGLLQQKLHPSYISSYRFQPAPGDTLTGGFSCTYRYKDKLYAGR
                     FSLYKGLAIINPFTMQQEKEISFYSGNNSWNEIRSIEMYHPDTLWLGTNAGILWFDTK
                     TNHYGKVLDEYRYPWATDLSAILAPPRNDGYAWICSQLGGLVARYNIPSRTFTIYTSG
                     TKPALPFDKVKSIIYDSYGDVWIAGHSLTRWNNKKQLFDTTITVYDGANKFNDDILAL
                     SADDNGSLWLHNAFNGLLEYRIAEKKFLSYTMKDGLPSDVLQSFSPVIDHNLWIGSNN
                     HLTRFDTRTKKIVIYDHHDGLPEHKPTGRKIWLDKEYNYLYLCADEYIVKFPLQSVSY
                     PDNSSGLIIQELVINNKKAFFQPGNEMNLKYNENNLLIHYSIINFEKNNYQFSYKLNN
                     EEDWVNLGQQRSINLTNLQPGKYSIQLKATGTNGDEKIKEFALFIHPPFWKSTWFLLT
                     CGLLLTGILFYLYKNRIKQVRKKANIDNLLAQTEMKALHSQMNPHFVFNSLNSIREMI
                     LNNENKEASHYLSKFARLIRITLEQSGQSFISLRNTIDYLHRYLEMEKIRNNDFTYSL
                     TTDTLMDMDETVLPPMLIQPFIENAIWHGVTANRKNIIIQVNFRKENEQLVCTVEDDG
                     IGINKSLSNKMSNESTHTSVGIANVQDHIRLLNEKYNLQCSILIKDKQDIAGPGVSGT
                     LVTLRLPYKLQDE"
     gene            4538..5512
                     /locus_tag="PLO70_02955"
     CDS             4538..5512
                     /locus_tag="PLO70_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73441.1"
                     /translation="MIKIFIRIYFLLLFLNLGLSRDLFSQNVGIGIANPVRAKLEVNG
                     TSGIGSTSAIFGGDGTGISFQRNWPTIGFNQYRDNTAGNGKYMANGFAAIQLFDPTTG
                     YMYFDMFSNGSANTHTNLGTRALSISNTGNIGIKANPANASLNVQKGTNFDGAAVFGG
                     TNYSSFFGYAETEDTYIRGGKYYSTVFINDIAYGNVQIGNGSTKVGINTLPFAPATAL
                     EINGGLSLRTALSSVANGGTVVVGDRSFIAVYSLTPGYSYIHLSDGLSAGQMLILMGD
                     PANGPLGPFLTDELNLELHDTFFLAPHNTVTLIWTGAKWVELCRSINT"
     gene            5551..6588
                     /locus_tag="PLO70_02960"
     CDS             5551..6588
                     /locus_tag="PLO70_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQZ73442.1"
                     /translation="MKYILLLYYALSLIPGFSFSQNVGIGTATPQARLHVTDSAVLFS
                     AVNDVPFIAGNPPQEGPGRRFMWYPDKAAFRVGYVSGTQWDKNITGQYSFASGYGSTA
                     SGVASTAMGYGTATDGFASTAMGNGTSANGTYSTAMGNGTSANGNFSTAMGNYVSTSG
                     FAGAFVIGDNSTTTVMQSFVANGFRSRFAGGYRLLTNSAANLGVVLVPDATSWSAISD
                     QRLKENFLPVDGEIILNRIALLPLSTWNYKAQALKTSRHYGPMAQDFYAAFGKDSLGY
                     IGCDTLINQQDFLGVNLIAIQALEKRTQKIEQLETENREQQIILLQLKNEIDLLKEQN
                     KLLVELIKNKN"
     gene            6593..7639
                     /locus_tag="PLO70_02965"
     CDS             6593..7639
                     /locus_tag="PLO70_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73443.1"
                     /translation="MHKPVLICYFSLLISILSLPVDSYSQNVGVGTNAPHAKAILEIK
                     STDKGVLFPRLTTAQRNVIVSPPDGLHIFNTDERCLNYYDSTNQIWNCYCNGCQTIVI
                     TIASNMCTVDFNAMYASISPSKKYLINILAGVVISGCDPGDTALSFNNIPYNADITIN
                     NYGTIAGGGGKGGNGAINRGCMFLDVVAAPGQNGGFAISTRDGIPVTINNYGVVAGGG
                     GGGGGSSGVNTTSSWGGGGGGGAGIVTGSGGTAGGLFVSSGAPISACVGLISYLGQNG
                     VAGLTTTGGSGGAGAGGGFSGGNGGGRGQAGQNGAGLIATIASGGAAGKAIGGGTDNS
                     ILNISGGQTYGHVD"
     gene            7848..8762
                     /locus_tag="PLO70_02970"
     CDS             7848..8762
                     /locus_tag="PLO70_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73444.1"
                     /translation="MKKYLLLIIFYLQHITFSYSQNIGVGNSNPQEKLDVSGNINVTG
                     TIKTNGVDGTAGQVLMKNNNNLLSWATICDFKSMATFIDTSVLSSQWNIPAGVTKIWI
                     EMWGGGGAGMLAGGGGGGYLSIFRNVTPGNNLVITVGKGGKDLPSSGFNQSGGSSYVV
                     SGSAGINAYAFGGTGAFTSTGAYNQDLPGTGGVILPAGGGGGHDGFYGQGGQAGSPLR
                     TEYFLLPSNQYNRVDNYGNGGNAANTQNTAGIGGVLTRNINGAVTTNLALSPARSSGF
                     PGGGGGGSADGFINTNRAGSNGMVIIHY"
     gene            9224..10342
                     /locus_tag="PLO70_02975"
     CDS             9224..10342
                     /locus_tag="PLO70_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73445.1"
                     /translation="MKMIIILLPLLTGYAFAQNVGIGTTAPAEKLEVKNSLRSTLKIS
                     SASYVDTSELLLSNRTNGNSGTDFSIKSIREEGLHFSSLSDLPANTATNSLVIRPGGN
                     VGVGIVPQFKFHVNGASRFNGLTQIDGLNLFEFGAGVPGKELNAGKIGYNAFGQNALT
                     FVGAGTNASNRAIYFFAEGGTTMSGPLNIGGTFRVNGNPGSAGQVLTSTGAGSPEWQN
                     SSYSNNTRFSVVLDETPTLLSGNVVFAATHYNLDPANVIIGASSITINKAGLYHFDIV
                     AYGELSYSPAPTGSYPRANLSLNVAAYANPIRLIGLRIMDPTSDAKLGWQFHEAVSIN
                     VYIPAPTTISLSYNYSSASPGGGFPSFNGNLTGSLISE"
     gene            complement(10392..10793)
                     /locus_tag="PLO70_02980"
     CDS             complement(10392..10793)
                     /locus_tag="PLO70_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HQZ73446.1"
                     /translation="MADISIVLFDGVCNLCNSSVQFIIKRDKKKQFLFAPLQGKKGQE
                     IFSKHSLPAGELNSFILAEGDNIYIKSTGALRMLKKLGGPWSLLYAFIIVPPFIRDGI
                     YSWVAKNRYKWFGKKDSCMIPTPDLKERFLD"
     gene            10871..11371
                     /locus_tag="PLO70_02985"
     CDS             10871..11371
                     /locus_tag="PLO70_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73447.1"
                     /translation="MKSTFLLFITIVFIQSFIYSQGKYAGSKKSLIGQVYTDYLELPA
                     FKGWTFMEGGVANSLSDPEMITSNVFKKGTSYVVFFSIREDTASGQFTIVDALEIKGV
                     QKGWTVRSSFCRQNKIENNYIIAWGKETTNEYIKLIKKAWRFNPDKRRIEIIPVKGID
                     CMNIGC"
     gene            11401..13122
                     /locus_tag="PLO70_02990"
     CDS             11401..13122
                     /locus_tag="PLO70_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HQZ73448.1"
                     /translation="MKLNSKSPQNLLLTGLLLFQTSMLFSQQPPLPAKKVEPRKAIIP
                     TKPIVKKDSVTYVATPARVEVNTRIATADEIYINQGTFLLRSGSDYRYLTIKDVVPAT
                     GSVAFLWSFVNYPVQQEWSFIPKPGGFYKIKSSSGFYLTQKRLFAPSMDPESAEDSQL
                     WQLIETGDGYYHIKSKTNKYLVVTDRRKRDGGLVSFANSVTNDLKQKWHLIKRDNDSR
                     KLTSFNPETNGFRFVNTFNGEDIIRWGGLCGGMVYSALDYFRNRIPIPTQTYTPANAT
                     PLQSYIYQRQQHSMWNVNEKWSELEVANNFRAGEIFRWGIQGTGGGRLEELKNAIDAG
                     KSVPIGLFGGGVPSINGGSGGNHVVLAVGYSLGRYQGDFGNRAGEYKILAYDPNMRNR
                     MVTLVPDLAGQCFFEVENGKAWRTYFVNTRYDNEHTPPRDIPNFPEREPDGSIRHLYV
                     EFATGNDDLRGGNDNVSITVQYADGTTQAFPNVNNGARWVDKSWQTIHLELNRPVRKA
                     DIRHFRLNTSFGSDIFSDDWNLEWFRVTTGFGGINIAQSFPPEGSEYIFRFSGDERRQ
                     MHVIRVE"
     gene            13187..14458
                     /locus_tag="PLO70_02995"
     CDS             13187..14458
                     /locus_tag="PLO70_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73449.1"
                     /translation="MKYFGLIIAKFLLIVNFVIAQDPPRRDTAWGDWPDQPYYNGVLS
                     APVVVGESVAEIISPAGQKLCFDKKVRIKSMISSGAVEQCMYINTVKGYVGILPPQRG
                     GGGPGLCDIKTDDAKFLFYVLGLKGNAYTFKNAEKNGTIEHWVSTGNTQAHQVNLTSP
                     SGNYTVHKKSERRGYCGDKIKTWAYKYDNPSSPVYFLFGKTFPEEINVSTNKYIGNFG
                     VGYQFTDKGLYIIMEMESGSYSCKITELEEVSVCFDPAPFKIAEDEFFMKASAGIQKE
                     RDKIARDEARVGDDCVSQKMAVIEFRKEQVRLQEARLYTSQQGNVYQDPATQQAMAQM
                     MDHGAMTKQMILENEVKICQAQRRLSQTSNQSSRERYQQRISCLGSQNDQLRSLEGQL
                     RAIDIQYVSEPGRAFAEKSKKFMQGLPRTCD"
     gene            complement(14480..15202)
                     /locus_tag="PLO70_03000"
     CDS             complement(14480..15202)
                     /locus_tag="PLO70_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ73450.1"
                     /translation="MGRIFEVRKASMFARWDKMAKNFTRIGKEIIIAVKAGGPDPNSN
                     AALRRCFQNARSVGMPKDRVEAAIKRAQGKELVNYDEILYEGYGPHGVAILVETATDN
                     HVRTVANVKSCFTKGHGTLGNSGSVSFQFKKMGVFKLKPEGLNAEDMELELIDYGLEE
                     LGEGTGENGEDILVVRCGFTDFGNMQKALEDKGITPISAELEWIPNVTVPVNDEQAAD
                     VSKLIEKLEQDDDVQKVFHNMG"
     gene            complement(15322..16863)
                     /locus_tag="PLO70_03005"
     CDS             complement(15322..16863)
                     /locus_tag="PLO70_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQZ73451.1"
                     /translation="MSLFRKKSLDHMLIQVADSEKGLKRTLGAGNLIALGIGAIIGAG
                     LFVRTAAAAGQAAGPAVTISFIIAAIGCAFAGLCYAEFASMIPIAGSAYAYSYVTMGE
                     LIAWIIGWALIMEYALGAATVSIAWSEYLNKLLGGAIPYEWCHSPFESFTDSAGVHHS
                     GILNAPALIILLALTLLLIKGTQESAFVNAIIVFIKVAIVILFIAIGWQYIKPENHTP
                     YMIPEGTEAVKDQAGKIIADYSSWNKHGWGGILGGAAIVFFAFIGFDAVSTAAQEAKN
                     PKRDMPIGILGSLVVCTILYILFGYVLTGVAPWKDFVDPEKGREASVAYAISTYMTNY
                     KWLADAVTVAILAGFSSVILVMLMGQSRIFYTMSNDGLIPKAFGQLHPKYKTPYKANW
                     ILFVFVGAFAAFVPGHVAGDLTSFGTLFAFVLVSIGVWILRVKNPTIERPFRTPMVPV
                     VCSLGAIICIAMIVGLDTQTLKVAFMWMAVGLVVYFAYSRKRSKLRNFADMLPTADDF
                     ESTKK"
     gene            complement(16891..18543)
                     /locus_tag="PLO70_03010"
     CDS             complement(16891..18543)
                     /locus_tag="PLO70_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HQZ73452.1"
                     /translation="MLKNISKIFYMMMTAIAVPFLSSAQDTPAPPKGLDERINEWFQP
                     ITDAWSSFVFYTINLGGVQVPLVVILLVCGALFFTLYFSFVNIRRFGLAIEVVRGKHD
                     DIEKDANNVKDPNVNVVDGDIVKTIKDESHHGEVNHFQALATAVSGTVGLGNIAGVAI
                     AIAIGGPGATFWMIICGLLGMSSKFVECTLGVKYRDIDKNGVVYGGPMYYLTKGFKEL
                     KLAGLGKFLAILFAILCVGGSLGGGNAFQSNQAAQQIAAMTGMQGASSGFFIGLALAI
                     ITGIVIIGGIKKIATVTEKIVPIMAIIYIVASLIIIFANFSFIDDAFSMIFKEAFSPR
                     AGLGGLMGVLIVGFKRAAFSNEAGAGSASIAHSAVKTKYPASEGIVALLEPFIDTVVI
                     CTMTALVIIMFNKDGLMFPYGGDGKGSVVLNATGGSTSGVDLTSMAYNEVIPHFSYVL
                     TIAIVLFAFSTMISWSYYGLQAWKFLFGRGKIADLSYKFLFLAFVVIGASVSLSAVTD
                     FSDAMILAMVFPNMIGLLFLFPRVKEELNRYLKAIKESKGKK"
     gene            18709..20109
                     /locus_tag="PLO70_03015"
     CDS             18709..20109
                     /locus_tag="PLO70_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQZ73453.1"
                     /translation="MNRRKAGLLSLLLFTVVALIHFLHMQKWIILQEVVLMTVRWIFI
                     ASLVLFAVYKKSLTTWILVAMAIGVEIGLDFPAFSQNLQFLSKIFLRLVKTIVAPLLF
                     ATLVVGIASHANLKQVGRMGWKSLLYFEVVTTIALLIGLVFINITKAGIGIKVPEALL
                     KELPEVKVKGWQDHIIDIFPENIIKSIYEGNVLPIVLFSVVFGIALALLNEQKKKPML
                     NFAESLAETMFRFTNIIMYFAPFGVGAAIAVTVGHLGIDILKNLALLLVTLYLALIAF
                     LILVLLPIAIWVAKIPIKKFIQAIREPVSIAFATTSSDSALPKALENMEKFGVPRKIV
                     SFVIPTGYTFNLDGTTLYLSLASVFVAQAAGMDLSFGEQLMIGLTLMLTSKGVAAVPR
                     ASLVILIATATTFHFPLWPIMAIYGIDELMDMARTSVNVIGNTLASCVIARWEGEFDD
                     DKALAFNEQEADAIRD"
     gene            20370..21653
                     /locus_tag="PLO70_03020"
     CDS             20370..21653
                     /locus_tag="PLO70_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQZ73454.1"
                     /translation="MKKVPLYIKIIAGMLAGVLFAIVAIQFSGGVKFTRDWIKPFGNI
                     FINMLKLIAVPLILASLIKGVSDLKDISKLSRMGLRSVVLYLFTTVFAVCLGLVIVNI
                     IKPGASISEETRTQMLSAYGGDANKKQQEAVQQQGSGPLKFLEDIVPDNIINAASTNS
                     TMLQVIFFALFLGISLILIPAERAKPVKDFFDSFNEVILKMIDLIMKIAPFGVFALMA
                     ALVAESPGFEIFKALGKYALTVLIGLVILIFIFYPVLIFLFTKRNPIQFIKQVYPAQL
                     LAFSTSSSAATLPVTMETVEKRVGVDNEVSSFVLPIGATVNMDGTSLYQAVAAVFIAQ
                     AFGMDLGLTAQLGIIVTATLASIGSAAVPSAGMVMLVIVLAQAGIPEAGLALIFAVDR
                     PLDMVRTVANVTSDCTVATIVAKSIGKLEDPHPVV"
     gene            21673..22431
                     /locus_tag="PLO70_03025"
     CDS             21673..22431
                     /locus_tag="PLO70_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQZ73455.1"
                     /translation="MFELLDAFHWTQLLQPQFYVENGGLWLLLFVVFAETGLFVGFFL
                     PGDSLLFVAGIYAHEITQKTGEVVPGLAYQFLDLIGMGHIRNEWVDLFVLVGLIAFMG
                     ILGNTVGYWTGRKVGPAMYQWRDRFLFKKRYLHQAHDFYEKHGGLAVIGARFLPFIRT
                     FAPIVAGIVQMDRKKFHFFNIVGSIAWVFSMIFGGYFLQKWILSQFGFDLKEHLELIV
                     LGIVLVTTAPVIIKMISGRKKKPAETTGGDSKQS"
     gene            22435..23694
                     /locus_tag="PLO70_03030"
     CDS             22435..23694
                     /locus_tag="PLO70_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ73456.1"
                     /translation="MNQAKPVSIFNIAVIVAALGYFVDIYDLLLFTIVREPSLAGLGV
                     DLTIKKDVIAASTKVINWQMWGLLIGGIIWGVMGDKKGRLSVLFGSIILYSIANFFTG
                     FVQTVEQYAFARFAAGIGLAGELGAGITLVSELLPKNKRGIGTSLVAGIGLFGAVAAY
                     FTFKFTDDWRLCYKIGAGLGIMLLFLRIKVAESGMFSQVKNQNISKGNLLMFFNNGKR
                     FKKYILAILIGMPTWFVIGILVNLSNRFATEFYGENKIESGRAIMYAYAAIAIGDILV
                     GLVSQYFKSRKKALYLFYFFAIVSGIYFFSGYVQSDTSMYIACAALGFSTGFWAIFVT
                     MGAEQFGTNLRATAATTIPNFVRGSLPLINLMFVGLFQQSIGWSFVKSAMITGIIVML
                     ITLIAAYFTEETFHKDLNYTEPEDIMP"
     gene            23705..24697
                     /locus_tag="PLO70_03035"
     CDS             23705..24697
                     /locus_tag="PLO70_03035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQZ73457.1"
                     /translation="MAKFLLIRFSSIGDIVLTTPVIRCLKKQVPDAEVHFLVKDSFRS
                     VVEHNPYIDKVHVLAHSWELMMEELKTEDYDYIIDLHHNTKTLRVKNALKKKSYSFYK
                     LNIEKYILTSIKINILPRVHIVDRYIKTVESFGVKNDGLGLDYFISPADETSKEDIPV
                     SHSAGYIACVIGAAHGTKRWPVHKWMAFCKEIDHPVILLGGKEDASNGDEITSVDPIK
                     IYNACGKFKLNESADLVRKARLVISNDTGLMHIAAAYKKQVISLWGNTVPEFGMTPYY
                     GDSAISSIPLQTKVWCRPCSKIGYDKCPLGHFKCMEKITVDELLERVRQKIALI"
     gene            25093..26037
                     /locus_tag="PLO70_03040"
     CDS             25093..26037
                     /locus_tag="PLO70_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73458.1"
                     /translation="MIYTTIYIFFLVACSLDAASRQPVSHIDSFRLYGDVKVTIDKPA
                     DFKKNKETIITFFALPNGNTTEQTMGKRMLPGDDLHFDIQHIRAQTKFIRQQMKKKNF
                     VVIYLENNYKSWPAWKQKHPGYRHEIPHLIDSLAGLSKSRKRSVYLNGHSGGGSFIFG
                     YLAGVERIPATIKRISFLDSDYAYDSSYYPKLKTWLQQVPGSCLNVFAYNDSVAIYNG
                     KPVVSPTGGTWYRSRLLLSHLRNDFIFSELITDSLIVNKSRDHRIQFYFKVNPNQGIY
                     HTQQVELNGFIHSVLCGTRYDSQGYRYYSGRAYADLIE"
     gene            complement(26047..26472)
                     /locus_tag="PLO70_03045"
     CDS             complement(26047..26472)
                     /locus_tag="PLO70_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73459.1"
                     /translation="MLRSGFLFMLCLTCLIFQTDASPIREFKAEKILLITLRETGLIT
                     VGNDRVGSDELARYIQERLFKSYMGTGLMHHKIKLVKTDPHIPDLVTDAVINEIKEGQ
                     KRALTELCLQKHRKLYENLSSKQQEKLKKQFPVLFQTSY"
     gene            complement(26569..28470)
                     /locus_tag="PLO70_03050"
     CDS             complement(26569..28470)
                     /locus_tag="PLO70_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQZ73460.1"
                     /translation="MHYVSVEGLTKSYGIQPLFSDITFHIEEGDKIALVARNGTGKST
                     LLRILAGKETADEGKVWVNKDVDVVLFEQEPTFVEEKLVLDNIFFHNHPIINAIKEYE
                     VISEEENAEKLSAAIIKMDELGAWDFDTKVKQVLEKLNIHHLNQPVSTLSGGQRKRVA
                     LAKTLIDIGFEHKHVLLIMDEPTNHLDVEMVEWLEHYLDKEKVTLLLVTHDRYFLDTV
                     CKEIWELDGSNMYVYKGDYENYLEKKAARIENDLSSIDKAKNTYRKELEWMRKQPKAR
                     TTKSKSRQDNFYEVEKKAKQQIIDEQLQLNMKMSRLGGKIIELKKIYKSYGDKILLKG
                     FDYTFKKGERIGVVGKNGVGKSTFINILQGLEKADSGKINIGDTVIFGNYSQQGLIVK
                     EDIRVIEFVKNIAENFPLATGGSLSAAQFLQLFLFDPDKQYTYISKLSGGEKRRLHLL
                     SILFRNPNFLILDEPTNDLDLPTLGVLENFLSEFPGCLLIVSHDRYFMDRLVDHLFVF
                     EGDGMIRDFPGNYTQYRQATANGQSLTEKNGASYIGEIEKPVSSANQDQVTAGKKRQL
                     SYKEKREFELLEKEISDLSKEKISITEKLNNGNTPFGELQQLSNRIGDVTRLLDEKEL
                     RWLELSEIQ"
     gene            complement(28486..29136)
                     /locus_tag="PLO70_03055"
     CDS             complement(28486..29136)
                     /locus_tag="PLO70_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQZ73461.1"
                     /translation="MYLKDYYQILELEPSASLPEVKKAYRKLAKQYHPDKNNNDPYAT
                     ALFADIKEAYEMLTDPAKKEYYLQQRWYHQSTGNRKTQEVITPVTVLKQALELEQYVS
                     KIDVFRMDKMGLQQYIMDLIPDTSIEKLQQFNEPDTIRQIVSILLHAIKPLPGQYTKD
                     IFIQLHKLSANEPIATEKLNIFIRQRQKKNNREKYSLVVIIILTIIISLFIYLLGR"
     gene            29281..30051
                     /locus_tag="PLO70_03060"
     CDS             29281..30051
                     /locus_tag="PLO70_03060"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQZ73462.1"
                     /translation="MDTPNKKNRREFLKTTGKAGLAAGLSATILPVLASVQTTQFIAA
                     GGAEDVLYIQRPLPYAFKTLEPVIDAMTMQIHYMKHAAAYANNLADAVKAEGVDTEKE
                     QLRFLLGRISKFSSKMRNNGGGHYNHEFFWKGMTSPVESNNPSPGLLKDITRDFGSFE
                     AFKTQFSDAAKNRFGSGWAWLVKTADKKLVVGSTPNQDNPLMDVSDLKGIPLLGLDVW
                     EHAYYLKYQNRRPDYINAWWSTVNWKIIQQRYEIAEMK"
     gene            complement(30052..30903)
                     /locus_tag="PLO70_03065"
     CDS             complement(30052..30903)
                     /locus_tag="PLO70_03065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQZ73463.1"
                     /translation="MDQQIINLYDEYTHKPLNRQEFLRRLAMLAGSTAAAMALLPLLE
                     VNYAHAAVTPQDDLLIERISYQGINSDMQAYVARPKEEKKYAAVLVIHENRGLNAHIE
                     DVTSRAAKAGFLAIAPNALSSLGGTPENADEARTKFQQLKPEDNLQNFIRVFDYLSAR
                     KDCNGQFGCVGFCWGGAMSNNLAVNVPALKAAVAFYGRQPVAEEVSKIKAAVQLHYGA
                     LDEGVNKGIPAYEEALKKNNIPYELYIYEGAQHAFHNDTAPTRYNETAAKLAWQRTIE
                     FFNKYLQ"
     gene            31198..32637
                     /locus_tag="PLO70_03070"
     CDS             31198..32637
                     /locus_tag="PLO70_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73464.1"
                     /translation="MKRIMKIAALAFVLVLGSSLRQTASAQYYDGQREVSYQTFYDEL
                     SPYGRWIDYPGQGYVWVPDAGPDFRPYSTNGHWVWSEDAEWMWVSDFDWGWAPFHYGR
                     WDNDPFYGWYWVPGYEWSPAWVAWRDGGDYYGWAPIRPGINININFNIGGYSPPYDFW
                     VFAPRRYITSPRIFDYYVDRRRNVTIIHQTVIINNFNYGGNYGYRTGPRRYDAERYCG
                     RIEPVRFRDSYRPGRTQFRNNEVSFYRPNIRRDDNRSISPRQFERYDRNNAGVRNGNN
                     DNSFRGNGNDIARNDRSNRPDNRDRADNSPFRRNDNSVRQPQSNGGNNLPDRRRIDRT
                     DNNSSQPNREGNTRVIRPTERPDRNSNPGNNNNERRQFERRNDNGNTNSPVRQPEVRN
                     NVPQRQAPRVEQRRSQEQPRQFERRNDISSRQPEARNNNSPQRQTPQFDQRRTQEQPR
                     QFERRNESNNRQERNSNNNGNSSSRNRRG"
     gene            complement(32704..35571)
                     /gene="gcvP"
                     /locus_tag="PLO70_03075"
     CDS             complement(32704..35571)
                     /gene="gcvP"
                     /locus_tag="PLO70_03075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQZ73465.1"
                     /translation="MSLLEQQSNEFIPRHIGPNEADTKAMLKTIGEASLSALIDKTVP
                     AGIRMTNPLSIPAAMSEHEYLKHIKEVSLRNKVFANYIGQGYYDTITPSVILRNIFEN
                     PGWYTQYTPYQAEISQGRLESLLNFQTMVSDLTALPIANASLLDEGTAAAEAMTMFFN
                     TLNKQDHIERPKFFVDAAIFPQTKDLLRTRAVPVGIEIVEGDYRNNVIDETYFGAIVQ
                     YPNEKGSIEDYRDFINKVHATGAFVVMATDLMALTLLTPPGELDADVAIGSAQRFGVP
                     LGYGGPHAAFFAAKDDFKRSIPGRIIGISIDAYGNRALRMALQTREQHIKREKATSNI
                     CTAQALLANMAAMYAVYHGPDGLKNIAKRIALFTQTVAEAIEDRGFELLSDTYFDTIV
                     VKTDTLKTIRDKAERQLINLRYIDNNHIGISIDETTDVTDLYDLINCFEDDKNPVAFD
                     IDQDAELQHIPSSLTRTSPFLSHPVFNTYRSESQMMRYIKQLENKDLSLNTSMISLGS
                     CTMKLNAASEMMPLSWPHWSKIHPFAPASQAEGYKYIIDELSNYLCEITAFDACSLQP
                     NSGAQGEYAGLLTIKSYHEANGDHHRKVMLIPISAHGTNPASAVMAGMKVVVVKALEN
                     GYIDVEDLKAKADQYSKDLAGIMITYPSTYGVYEETVKEITEIIHQHGGQVYMDGANM
                     NAQVGLTAPGLIGADVCHLNLHKTFAIPHGGGGPGMGPICVKSHLAKHLPGHVANGSS
                     HAVSAAPYGSASILLISYGYIRMLGNEGVKAATEYAILNANYMRARLDGKYDILYTNN
                     NGQCAHEFIVDLRPFKKSAEVEAEDVAKRLIDYGFHAPTMSFPVPGTIMIEPTESEDK
                     AELDRFCDALISIREEITAIEEGRADKKDNALKNAPHTQFVVTADEWKHAYSRQEAAF
                     PLHYVTLNKFWPSVARVNNTHGDRHLICTCEPVESYMVAEA"
     gene            35682..36167
                     /locus_tag="PLO70_03080"
     CDS             35682..36167
                     /locus_tag="PLO70_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQZ73466.1"
                     /translation="MQEKLLQNWEKKSGERQKEYKKFLNRADKNKVLKQLPLLNEEAF
                     SKIDCLQCANCCKNYSPRFTPPDIKRISKYLGMKEGDFKDTYLLTDSDGDNVVKALPC
                     PFLGTDNYCSIYEVRPNDCARFPYMDDDFMLKRQPLALKNSTFCPITYYVIEKLIAAG
                     K"
     gene            36269..36784
                     /locus_tag="PLO70_03085"
     CDS             36269..36784
                     /locus_tag="PLO70_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HQZ73467.1"
                     /translation="MKKAVVIKKSKAYPKPEFDNPPALQEAAVKYESLRVILGGRQTL
                     RQSLSGDFDLIALTREGIKKSTLKSLAEHLGISMETMSGLLHSSYRNIQRKDEDELLD
                     TLKTEKVLELAAFAQRGIEVIGSKEAFKEWLHSPIVALGNKPPLDFLDTSFGIQLVIK
                     ILGRLEQGVFS"
     gene            36788..37255
                     /locus_tag="PLO70_03090"
     CDS             36788..37255
                     /locus_tag="PLO70_03090"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQZ73468.1"
                     /translation="MELYRITQEIYAGDLSGNGAKLFGGRWNSEGLPALYAASTRSLA
                     LLETLAHTPAKMLQVKVYMLLTVIVPDTILPLVIELRKLPDGWDVPDIRPFTQKTGDS
                     FLLAKKNLLIAVPSVLLPEENNFVLNPLHPDAKKIKIVHSRRIHFDKRVVVNL"
     gene            complement(37317..37544)
                     /locus_tag="PLO70_03095"
     CDS             complement(37317..37544)
                     /locus_tag="PLO70_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73469.1"
                     /translation="MKIIVIIIAALIIWGIIVSSSGSKKNQQKDSGDNNSDAGTAAET
                     GHKDHPDADYNSTESDPGESGGESGGDGGGE"
     gene            complement(37550..38446)
                     /locus_tag="PLO70_03100"
     CDS             complement(37550..38446)
                     /locus_tag="PLO70_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ73470.1"
                     /translation="MNRRSFLQNTAFTMGAFTLAQHNILSALFQEPWKITMLRNDVGI
                     FTERGGTIAFLLSKDGTVVVDSQFPDQSKHLIDELKKKSEQPFKLLINTHHHGDHSGG
                     NISFKGLVEHVLAHENSKKNQENSARQNKSEDKQLYPDQTFTNTWCQKVGKEKICLHY
                     FGAGHTNGDSFIHFQHANIVHCGDLVFNRRHPYVDRNAGANMRSWINVLDKALNKFNK
                     KTIYVFGHAGTGYEVTGKADDLKVFRDYLGNVLKFVEGEIKAGKTKEEILKASAIPGS
                     DQWKGDGIQRPLTAAWEELTVK"
     gene            complement(38537..39781)
                     /locus_tag="PLO70_03105"
     CDS             complement(38537..39781)
                     /locus_tag="PLO70_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ73471.1"
                     /translation="MSDRFSIKNIFSTVINKEFTWFITARILFIAGLRMTPVLLGWRL
                     YEITGSKLALGFLGLSEVIPAIALALPAGVKVDKSNKHRLISICIAIYFFLMLGMLLV
                     TSQWMQDNYSRSIIEWSIYGLVFGTGAVRAYTGSAFNAFLAQLVPQNSLVKAVSINSM
                     VWLVAAVAGPAIAGLLMGYTNITIAFTPVCVLIGIAFFLFQRIKPKQVSWQGNSKTWD
                     GVKDGLRFVFKQKALLGAMSLDMFAVLFGGAVALLPAFANDILHVGPQGLGWLVAATY
                     LGNFVAIAWLTSHPLKGKQGKTLLYVVAGFGLCILVFAISQNFWLSFFALFAGGLFDG
                     VSVIIRGTVVQLFVPDEMRGRVSSVNSIFINSSNELGQFESGVAATAMGMVPSVIFGG
                     CMTLLVVIITWFKAPGLRKFEY"
     gene            complement(39778..40410)
                     /locus_tag="PLO70_03110"
     CDS             complement(39778..40410)
                     /locus_tag="PLO70_03110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQZ73472.1"
                     /translation="MNYHFITIEGNIGAGKTTLAHLLSKHYNARLILEEFADNPFLPK
                     FYENPGQYAFPLELFFMAERFKQQKELLQQKDMFQALTISDYLFTKCLLFAKVTLPDD
                     EFRLYQRLFDIIHQQLVQPDILIYLHAPVAKLQSNIKKRNRAYEQNIQDEYLFNIQET
                     YTHYIKQHNIKTLFVDASNADFLGNEKHLQVIYDALDKEYENGQHYITLP"
     gene            complement(40503..40985)
                     /gene="folK"
                     /locus_tag="PLO70_03115"
     CDS             complement(40503..40985)
                     /gene="folK"
                     /locus_tag="PLO70_03115"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQZ73473.1"
                     /translation="MNTAYLLTGGNLGERVHNLAMARELVEAQTGNIIAASSLYETAA
                     WGNTDQPAFLNQAIMIETPLNARQLIRRILKIEKKMGRVREEKYGPRLIDIDILLFNN
                     EKHNYQFLKLPHPEMQNRRFALLPLAEIAPEIIHPVLKKTITELLQECKDELEVKKYS
                     "
     gene            41049..42167
                     /locus_tag="PLO70_03120"
     CDS             41049..42167
                     /locus_tag="PLO70_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73474.1"
                     /translation="MKNSQQPYSQSRALWAITRASFKAIFSNPSAIIFSILFPIIFVL
                     IFGAFGSGGGVSYRIALTPDSDTTNDFYQGLKQTPVIRIVSYSDSALLNKDLQKGKLT
                     AILTLTRNEDTAQKPEITVKVNSTTASANTIGSFIPVLDYIRLRIETANAGIEQESIA
                     VEEPVIKEVRKYRQIDFILPGQLGFSILFATLFGVAFTFFNLREQLVLKRFYASPVNK
                     INILLGIGMSRLFFQLLSVVVLILFGHFFMNFTLQNGIFTFLEIIAMTIIMLFLLMGV
                     GLIFSSIAKSDTSIPLLINLFGFPQMMLSGTFFPIEVFPKWLQEICRVLPLTQYNDAV
                     RKISFEGLSIFECWKEIGILGIWIVIIYFITARVLKWE"
     gene            42223..42720
                     /locus_tag="PLO70_03125"
     CDS             42223..42720
                     /locus_tag="PLO70_03125"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQZ73475.1"
                     /translation="MRLLKLAIISFIVFFLLITAISLFIPAHVRISKATDINATKDSV
                     MLQVKNAANWKNWYPGADTLEVLKIDGVVKGILTKGQQGIIITGSDDSTVMAGNAGTA
                     PGKGQMGWNILQSRGKGPVTVQWYMDFRFSWYPWEKFSSLLLEKRYGLLMEQGLDKLK
                     KFLEK"
     gene            42770..43267
                     /locus_tag="PLO70_03130"
     CDS             42770..43267
                     /locus_tag="PLO70_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ73476.1"
                     /translation="MKNILLVLAVFAVFNSYSQQSTRKSPHDTVSSANTIVTYGRPFK
                     NNREVFGGLEKFGKVWRVGADEATTISFAKDVNFAGAGIKAGTYTLFAIPEEKEWTII
                     LNSQLGQWGAYGYEKNKDKNVASVKVPVMKLSNVVEQLTIRFAENGSLVIEWDMTQVT
                     VPLSF"
     gene            43361..44221
                     /locus_tag="PLO70_03135"
     CDS             43361..44221
                     /locus_tag="PLO70_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73477.1"
                     /translation="MTCLKRVLVSALSLSLVLTIQAQVTEGDTLLFQRDSVIELEPDP
                     TTIFFQQGEKTKISGKVMLVTKSRKAISLANFLKSMVADVTDFPLYTDHAFADMDNDG
                     KKELLVSNYTGGAHCCDEIYIFKNIGPNKYQHVAKLFAGNTIITKEKEFIYDFHEQFG
                     YFFTCFACGYTDTTDGAPVEIRSITLKYNKGKMVVTPGDQELKSIINDNLGKLGEQPY
                     EKLSDDIDQDNGLRKEFALNLVVHYYSFGRNVMETQKLFNKYYKYPDAKKVWAAFVKQ
                     MQYMKGDNDF"
     gene            complement(44222..45145)
                     /gene="queG"
                     /locus_tag="PLO70_03140"
     CDS             complement(44222..45145)
                     /gene="queG"
                     /locus_tag="PLO70_03140"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQZ73478.1"
                     /translation="MHTHTHFIKKTAGRLGFSYCGITRAHKLEEDAVRLEKWLQKGMH
                     GRMQYMENYFDLRIDPSKLVPGARTVITLLLNYYPAQEQQPEAPKISKYARGLDYHEV
                     IREKLKELIAEMQGHIGEIQGRGFVDSAPVLERTWAQKSGLGWIGKNGNLITKQSGSF
                     YFIATLITDLELEYDDPFVKDYCGTCTKCIDACPTEAILPDKVIDGSKCISYFTIELK
                     EMIIPDAMKGRFDNWMFGCDTCQDVCPWNRFSKPYDETSFAPIPEILNLSTKEWELMT
                     EESFKTIFKHSPLKRTKWKGIQRNLKFISGA"
     gene            45280..47292
                     /locus_tag="PLO70_03145"
     CDS             45280..47292
                     /locus_tag="PLO70_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQZ73479.1"
                     /translation="MRSKLYFLCIGLLFFSGNLFAQEKSNVKFGNVSEKDFNTKIYPI
                     DSNASAVVIADIGYSAIEGNTKGWFSLVYKHFKRLHILNKNGYDAANVSISLYADGDA
                     EEELNKLKAVTYNLENGKVVETKLDVKASVFKDKISKNRVVKKFTFPNVKEGSIIEYE
                     YTITSDFLQNLQPWEFQGAYPRLWSEYNLALPEFLGYVFLTRGYKKYDINDKKLRSES
                     FTILDSRGIGASERYQFTANVSDNRWVMKNVPALKEESFTSTIDNHIAKIEFQLSEYR
                     QPLTYRNIMGSWPKLANDLMEADFFGKPVTKDNGWLKDIVKPLIEGVNGNKEKAHKIF
                     AYVRDNFTCTNYSDLFAEQTLKNLEKSKSGTVAEINLLLTAMLRYADIEADPVILSTR
                     SNGMTYPLYPLITQYNYVIARIIIDGKTFYLDASEPRLGFGHLPVRCYNGHGRVINKT
                     ADAIELESDSLMERKSTTVFVVNDEKGNIVGSMLQNPGYYESFNLRERIKEKGKEQLI
                     TDIKKAFGTEILVSKFELDSLDKYEEVLGIKYDFDFTGEKEDIIYLNPMFGEGYKENP
                     FKSAERLYPVEMPYGFDETYTLQMDVPSGYEVDELPKSMVVKLNEENEGMFEYRISQS
                     GSGISFRSRIRISRAYFMPEEYEMLREFFNLVVKKQAEQIVLKKKK"
     gene            47296..49224
                     /locus_tag="PLO70_03150"
     CDS             47296..49224
                     /locus_tag="PLO70_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQZ73480.1"
                     /translation="MRNRFILLICLATLISLAGMAQSSYAVVAIPEALLKNASVIKRT
                     EDIRYEITEGNKASFYQKVAYTILDEQGDRWATHAEWYDKLRSVESFEGSLYDASGKK
                     IRSIKKGEIKDVSGNDDASLADDNRVKWHSFFYKVYPFTVEYETEIRFKGTMFSPDWI
                     PQQKGAMSVQHSRLTVLSPVSNPLRYKMFNYKGEPVVAEVKQGKSYTWEVKDLPAIKE
                     EFATPSWHEITTSVFLATEKFVLEDYQGSNASWKDFGKFVYDLKKERDELPEEVKQKV
                     RQLTSGVTEDKEKVRRLYEYMQQNTRYISIQLGVGGWQPFDAKYVASKRYGDCKALSN
                     YMFALLKEAGIRSVYTVIGSGNDNDYLMVDLPSSQFNHAILFVPQGKDTVWLECTSQT
                     NGAGYLGGNTGNRYALAVDENGGALVRTPHYGMNENLQIRSIKATLAEDGTLLVKSNT
                     HYSGMQQDNIHGLINNLSKDKVKEYLHEELDFATYDVNQFNYSQDKSILPVIDETLEI
                     TVSNYATVTGKRLFIVPNIMNKTYRKLSATDERKYDIDLGFEYKDVDSVEIELPKGYE
                     PEAMPQPVSISSQFGKYSNTVKLAGNKLIYYRSIEQYSGRFPAKTYPDLVKYYDAVYK
                     ADRSRVVLVRNEQPLKGF"
     gene            49266..>49824
                     /locus_tag="PLO70_03155"
     CDS             49266..>49824
                     /locus_tag="PLO70_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQZ73481.1"
                     /translation="MQKLLSAFFILCSVSCFSQQNPTVKFGKISIAEIERKNYDVDTS
                     AEAVVIADIGATQIKGNRNEWFSLEFRRHKRIHILKRNAYSHATVEISLYTDGKMEEQ
                     LDDVKAVTYNYENGKIVETKLDVRTGVFKDKLDKNRVVKKFTLPNVKEGSIIEYEYKV
                     TSDFLFNLQPWYFQGDIPRLWSEYSV"
BASE COUNT        14524 a         9617 c        10735 g        14948 t
ORIGIN      
        1 ggaactggta aatgaagaag cagtcaaaaa atgtgaacct gatttctctg ccatgaaaaa
       61 atactttgat aaaatgatta tcactgcgcc aggaaaagaa gtggactttg tgtcaaggtt
      121 ttttgcaccc ggagcaggga ttgatgagga tcccgttacc gggtcagctc attcccaact
      181 gataccattc tggagtgaga aattgggaaa aaataaaatg ctggcaaagc agttatcaca
      241 aaggggagga gatatctatt gtgaacaatt gaatacagaa agagtgatca tgggtggtga
      301 atgtgtcttt tacatgaaag gagtaataag tatctgatat atcagaattt gtaatattcc
      361 ttcattttcg tgatcagcat atcttttttt ctgcgggata cttcaacttc caggttattc
      421 gacaggagca cactgccccc ttcaccgcgg atatattctt tcacatgttc caggtttacc
      481 acaaatgatt tgtgaacccg aacaaaatta caatcagcaa gcaactcttc atattcatgc
      541 aatggttttg atgcacagat agaaacccgg ttggtaaaat gaaagtttgt atagttgctg
      601 ctggcctcac aatggataat atcttttatt tccactacct ggaacccttt cagggagggt
      661 atgcataatt tcattttttg cctgggtcct ttgtggtgaa tattgtgcaa aaaggtttcg
      721 agttgctgcc ctccgctttt ttcttcaatt cgtttacctg ccctttttac tgcttctgtc
      781 aacaggtcat cttcgatggg cttcaataaa taatcaatgg cactgaaatg aaacgcctgc
      841 aacatgtagt tttcatgggc tgtaacgaaa atgatctcaa aatctatcac cggtaaactt
      901 ttaagcagat caaacccatt tctttccggc attgcaatat caagaaatat taatccgggt
      961 tttaattctt ctatcttttc ttttgcttct gttgcattac cggcatagcc aataactacc
     1021 acttccgggc agtttaattg caatagctta tgcaaagagg tctgccctct gggctcatca
     1081 tcaaccagta aagtctttat cttactcatt cgtcctgtaa tttatatggc aaacgcaaag
     1141 taaccaaagt tccggatact cctggtcctg caatatcctg tttgtcttta atcaggatac
     1201 tgcattggag attatatttt tcattcagta aacgtatatg gtcctgaaca ttggctattc
     1261 caaccgatgt atgagtgctc tcatttgaca ttttgtttga taaagattta ttgatgccga
     1321 taccatcatc ttcaactgta cataccagtt gctcattttc ttttctgaaa tttacctgga
     1381 taatgatatt ttttctgttg gcagtgaccc catgccagat cgcattttca ataaatggtt
     1441 gtatcagcat cgggggcaat actgtttcat ccatatccat cagggtatct gtagtaagtg
     1501 aataagtaaa atcgttgttg cgtatctttt ccatttctag atatcggtgg aggtaatcaa
     1561 tggtgtttcg caaagaaata aatgattgcc cggattgttc cagtgttatc ctgataaggc
     1621 gtgcaaactt gctcaggtag tgggaagctt ctttgttttc attattcagg atcatttcac
     1681 ggatactatt caggctgtta aagacaaaat gagggttcat ttgtgaatga agagccttca
     1741 tctctgtttg tgccagcagg ttatcaatat tggctttttt tctgacctgt tttatacggt
     1801 ttttgtataa atagaaaaga attcctgtta ataaaaggcc gcaggtaaga agaaaccatg
     1861 ttgatttcca gaacggggga tgaataaaca gtgcgaattc ttttattttc tcatccccgt
     1921 ttgtaccggt agcttttaac tgaattgaat attttcccgg ttgcaaatta gtaaggttaa
     1981 tgctgcgctg ctgcccgaga ttgacccaat cttcttcatt gttcagttta tatgaaaatt
     2041 ggtaattatt tttttcgaaa ttaataatgg aataatgaat taataaattg ttttcattgt
     2101 actttaagtt catctcattg cccggttgaa aaaaagcttt cttattattg ataaccagtt
     2161 cctgtataat cagcccgctg ctgttatccg gataactaac ggattgtaag gggaacttta
     2221 caatgtattc gtccgcacac aggtataagt aattgtattc tttatctaac catatttttc
     2281 tgccggtagg tttgtgttcg ggcaggccat catgatgatc ataaatgaca atcttttttg
     2341 tccgggtatc aaaccttgtg aggtggttgt tactgcctat ccaaaggtta tgatcaatta
     2401 ccggactgaa actttgcaaa acatcagagg gtaatccgtc tttcattgta tagctcaaaa
     2461 attttttttc agctatccgg tattctaaaa ggccattaaa agcattatgg agccaaagtg
     2521 atccattatc atctgctgat aatgcaagta tatcgtcatt gaatttattt gcgccgtcgt
     2581 aaacagttat tgttgtgtcg aacaattgtt ttttgttatt ccagcgtgta agtgaatgcc
     2641 cggcgatcca tacatctcca tatgagtcat aaataatgct ttttaccttg tcaaaaggaa
     2701 gtgcgggttt tgttccggaa gtatagatcg taaaggttct ggagggaata ttgtaacggg
     2761 caacgagtcc gcccaactga ctgcaaatcc atgcatatcc atcattccgg ggtggtgcca
     2821 gtatcgcaga aagatcagtt gcccagggat atctgtattc gtccaacact ttaccgtagt
     2881 ggttagtttt tgtatcgaac cataaaatac ctgcatttgt acccagccat aatgtatccg
     2941 ggtgatacat ttctatggat cggatctcat tccagctgtt attgccgcta taaaaagata
     3001 tttctttttc ctgctgcatg gtgaaaggat ttattattgc caatccttta tacaaagaaa
     3061 agcgtccggc gtatagtttg tctttatacc tataggtaca actgaaaccg cctgtcagtg
     3121 tatcacccgg tgcaggttgg aacctgtatg aacttatgta ggaggggtgg agtttttgtt
     3181 gcagcaatcc gccactggta cccgcccata gtctattgtc tttatcaaca aacaggcgaa
     3241 cgatcttata cgaggagagg aatttttttt tattgaaata aatttgtcct gttttcctgt
     3301 caagattaaa tatataaaat ccccttgtac ggtcattgat cataaaacta ctgtcattca
     3361 actttgtgat cctgctttcc cagctgaaag cagacgatgt tggaaaaggc acaggagaag
     3421 tgatgacctt ctgcaattga tgattgtaat agatgatact gtctttcctt aaaaagataa
     3481 aaaagaattc atcgttgtta agctggtatt ttgtgatcca ttgaccaccc attgttatac
     3541 tgggatgtgc taatgctgca tattttattt cttcagtacc gggttccctg tatgatcttt
     3601 gttccgcatt atatatagcc ataccggatt cttcaaaata gacaacctgt tcacttttat
     3661 ttaagggaaa tatctcatgc ccgtaaagaa tccttttttt tccaatatcc ggcaatgtat
     3721 acgcatcatg cctgaacttg attgtaccgg atggattaaa aacataaaat ccggatgctg
     3781 tcgtaattgc atacgagtgg tctgccaatt caacggcatc ccaggcggca ttctggtagg
     3841 tgctgaaagc ggtgctgtcc gggatataaa atctttttaa ggaaaaatcc cttgtatcta
     3901 ttatttccag gccgccacca ctgatgatac ccagttgctg tggtcctatt attttaatat
     3961 tcctgatatc aatggaggcc agtggtaaat ccggtgatcc ctggtaaaag ttgttgaaag
     4021 aatgaccatc aaaccggttc agcccattgt tggttccggc ccatatatat cccaggttgt
     4081 cctgctgcag gcaggtaata tagttatcac tcaatccgtc ttttaccgtg taacgcacaa
     4141 aatcactttc ctcatattgt gccaggcagg gttgtataat tgatgtgaga aaagataaaa
     4201 gacaaagaaa cgttttataa ggaaagtttg gttgcataaa gattgtcgtt cttcctgtgt
     4261 ttctaaagtt aaatctataa ttgatataaa cagatagggt taacgaatga atctgtgatg
     4321 ctggaaataa tgctgtaaat gacaccagtt atcgtttgaa atcaaccgtt ggtgcaaagc
     4381 aggtataaaa attttatcaa agcaggttcg ccgtacaatc aaacaaagcc gccgtaaatg
     4441 caatcatacc tgctgttggt aaaaactggc gtaatcttat tcttgttaag ctgtaaattc
     4501 caataggtaa ttcaaaaatt tatccgaaaa taattgtatg ataaaaatat ttatccgaat
     4561 ttatttctta ctcctttttc taaacctggg tttatccagg gatttgtttt ctcaaaatgt
     4621 tggtattgga atcgctaatc cagtccgggc taaattagaa gtaaatggta cttcaggcat
     4681 agggagcacc tctgctattt tcggaggtga tggcacaggt atatcttttc aaagaaactg
     4741 gccgaccatt gggtttaatc aataccggga caatacggct ggtaatggca aatacatggc
     4801 aaatggattt gcagccatcc agctcttcga cccgaccaca ggttacatgt atttcgatat
     4861 gttttccaat ggtagtgcaa atacacacac taatttaggt acaagggctt tatcaataag
     4921 taataccggt aatattggga tcaaagcaaa tcctgcaaat gcatccctga atgtacaaaa
     4981 aggaacaaac ttcgatggag cagcagtatt cggaggtaca aattatagtt ctttttttgg
     5041 ttatgctgaa acagaggaca cctatataag aggtggaaaa tattatagta ccgtattcat
     5101 caacgatata gcctatggca atgttcagat aggtaatgga agcaccaagg taggaataaa
     5161 tacattgccc tttgcaccag caactgcatt agaaattaat ggtggtttgt cgttgagaac
     5221 agcattgtcc agcgttgcga atggtggaac tgttgttgtt ggtgatcggt cctttattgc
     5281 agtatatagc ttaacaccgg gctatagtta tattcattta tcagacggtc tttccgcagg
     5341 tcaaatgttg attctaatgg gagatccggc taatggccct ttgggtcctt ttttaactga
     5401 tgaattaaat cttgaactgc acgatacttt ttttttagcg ccacacaaca ctgttacgct
     5461 tatctggact ggtgcaaagt gggtggaatt atgccggtca attaatacat aatagataag
     5521 aaaatgattg ataagggaaa ataaattgcc atgaaatata tactattatt gtattatgct
     5581 ttatcactca ttcccggttt ctcattctca caaaatgtag gaattggtac tgctacaccc
     5641 caggcaaggc tgcatgtaac tgacagtgct gttttattca gtgctgtaaa tgatgttcca
     5701 tttatagcag gcaatccacc gcaagagggg ccgggccgca ggtttatgtg gtatcccgat
     5761 aaagcagctt tccgggttgg gtatgtaagc ggcacccagt gggacaaaaa tattacaggg
     5821 caatattctt ttgcttcagg ttatgggtca accgccagtg gcgtagcctc tactgcgatg
     5881 ggatacggca ctgctactga tggttttgct tctactgcga tgggaaatgg cacttctgct
     5941 aatggcactt actctactgc gatgggaaat ggcacttctg ctaatggcaa tttctctaca
     6001 gccatgggca actatgtttc taccagcgga tttgccgggg cctttgtaat aggtgataat
     6061 tcaactacca ccgttatgca atcatttgtt gccaatggtt tccggtcaag atttgccggt
     6121 ggctatcgtc ttttaacaaa tagtgcagcc aaccttggag ttgtccttgt accggatgca
     6181 acttcgtgga gtgctatcag tgatcagcgg ttgaaagaaa attttttacc ggtagatggc
     6241 gaaataattt taaacagaat agcattactg ccactttcaa catggaatta taaagcgcag
     6301 gcattaaaaa catcgaggca ttacggcccg atggcccagg atttttatgc agcatttggt
     6361 aaagacagtc tcggatatat cggctgcgat acattaataa accagcaaga ttttctggga
     6421 gtgaatttaa ttgccataca ggcattggag aaacgaacac aaaagattga gcagttggaa
     6481 acggaaaaca gggaacagca aataatactc ctgcagctaa aaaatgaaat tgatttgctg
     6541 aaagaacaaa acaaattgct ggtagaatta ataaaaaata aaaattaatg ttatgcataa
     6601 accggtttta atttgttatt tctccttact tatttcaatc ctgagtttgc cggtagattc
     6661 ttattctcag aatgtagggg tgggtacaaa tgcaccacat gcaaaggcaa tactggagat
     6721 aaaatcaact gataaaggag ttttattccc ccggcttact actgcacagc ggaatgtaat
     6781 tgtcagcccg cctgacgggt tgcatatttt taatactgat gaacgctgtc ttaattatta
     6841 tgattcgact aaccagatat ggaattgcta ttgtaacggt tgccagacga tagttatcac
     6901 tattgccagt aacatgtgta cggttgattt taatgcgatg tatgccagta taagcccttc
     6961 taaaaaatac ctgattaaca tattggccgg agttgtaatc agtggctgtg atcccggcga
     7021 tactgcttta tcattcaata atataccata caatgcagac atcaccatca ataattatgg
     7081 aaccattgca ggtgggggcg gcaaaggcgg taatggagcc atcaaccgtg gatgtatgtt
     7141 ccttgatgta gtagctgctc ccggacagaa tggtggattt gctataagta ccagagacgg
     7201 tatacctgta acaataaaca attatggagt agttgcaggt ggtggcggtg gtggcggtgg
     7261 aagcagtgga gtaaacacta catcaagttg gggaggagga ggtggtggtg gtgctggaat
     7321 agtaacaggt agcgggggaa cagcaggagg tctttttgta tcaagcggcg caccaataag
     7381 tgcttgtgta ggcttaattt cttacctggg ccagaatggt gtagccggat tgacaacaac
     7441 tggaggatct ggcggagcag gagctggcgg tggcttttca ggaggcaatg gtggcggtag
     7501 agggcaagca ggacagaacg gggctggctt aatagcaaca atagcatcag gcggggcagc
     7561 aggtaaagca attggtggtg gcactgataa cagtattctt aatatcagcg gcggacagac
     7621 ttatgggcat gttgattaaa taaaggaatt taatttttaa ttcatgctgg ttcaatcagg
     7681 gaatcgttct tttattttcg ctgttggaat catgtaatta tcttttttgc caaacaactt
     7741 ataagagtac aatcaataat attataatag aacccgaata ttgatagcaa tttacaaaca
     7801 ggattttttt ttaaatattt aattagtgga caattaaaac gctttttatg aaaaagtatc
     7861 tcttattgat tattttttat ttgcagcata ttactttttc ttattcacaa aatattggtg
     7921 taggtaacag caatccccag gaaaagctcg atgttagcgg taatattaat gttaccggaa
     7981 caatcaaaac aaatggtgtg gatggaacag ccggacaggt gctaatgaaa aacaataata
     8041 accttcttag ctgggcaact atttgtgatt ttaaaagcat ggcgaccttt atagatacat
     8101 cagtattgtc ttcacaatgg aatattcctg ccggagtaac aaaaatctgg attgaaatgt
     8161 ggggtggtgg tggtgccggt atgctggcag gtggaggtgg cggcggttat ttatctattt
     8221 ttagaaatgt aacgcctggt aataatttag taattaccgt tggaaaggga ggtaaagatt
     8281 taccatcttc agggtttaat caatcaggag gttccagcta tgtagttagc ggctcggctg
     8341 gaataaatgc atatgcattt ggtggaaccg gggcctttac cagtacagga gcatataacc
     8401 aggatttacc cggtacggga ggtgttatac ttccggctgg tggaggtgga ggtcatgatg
     8461 gtttttacgg tcagggaggg caagctggtt cacctttgcg tacagaatat tttttattgc
     8521 cttcgaatca atacaaccgg gttgataatt atggtaacgg cggtaatgcg gccaacacac
     8581 aaaacactgc cggtataggc ggtgttttaa caagaaatat taatggagca gttacaacaa
     8641 atcttgcact tagtccagct cggtcttcag gttttcccgg cggcggtggc ggtggttcag
     8701 cagatggttt tataaatact aaccgggcgg gatcaaatgg catggtgata attcattatt
     8761 gataatgttt agactaagaa acctcccatt caataagtaa gtttgaatta ttcactggtc
     8821 gcagttacaa ttttttttgt agtgtcttcc taatgtataa tcacgaatgt gtatacatta
     8881 gtacaagtta attattataa aaaaggccat catcttttaa aattagctat gcgccagttg
     8941 ttttgctata atttttttcc atctgcctga ctaaataaat taaactcacc tggttcctgg
     9001 ctatagcttg ggaattattt aatgccagct attccctgat tggtaaaaaa aagatttttt
     9061 tcgtttgacc atgaattgaa tgcaactgtt gcagagataa cataaaccat tgctatattc
     9121 aattttgctg tcttttctta ttggttgtat ttttaattat taactcagtt ggatattcat
     9181 ttataaattg atatttttat ttattccatt aaactgtcta tttatgaaaa tgattattat
     9241 cctccttcct cttttaacag gttatgcatt tgctcaaaat gtgggcattg gaacaactgc
     9301 cccggctgaa aaactggaag tcaaaaattc gttgagaagt actttgaaaa taagttctgc
     9361 cagttacgtg gatacatcag aattgctgtt aagtaaccga acaaatggaa atagcggtac
     9421 agatttttca ataaaaagta tacgggagga aggattgcac ttttcttccc tttctgacct
     9481 gccggcaaat acagcaacaa attctttggt aatacgtccg ggtggaaatg taggtgtggg
     9541 aattgtcccc caatttaaat ttcatgtaaa tggagccagc cggtttaatg gattgaccca
     9601 aattgatgga ctgaatcttt ttgagttcgg agccggtgtg cccggtaaag aactgaatgc
     9661 cgggaagatc ggctacaatg cattcgggca gaatgcgctg acatttgtcg gcgccggaac
     9721 caatgcaagc aacagggcca tttatttttt tgcagaagga ggaaccacga tgagtggtcc
     9781 gctaaatatt ggtggcactt ttagagtaaa cggaaacccg ggaagtgcag gacaggtgct
     9841 tacctccaca ggtgctggtt cacccgaatg gcagaattct tcttattcca ataatacccg
     9901 tttttctgtg gtattggacg aaaccccgac attactatcc gggaatgttg tttttgcggc
     9961 aacacattat aacctggatc cggctaatgt tattattggt gcatcttcca ttacaattaa
    10021 taaagctgga ttgtatcatt tcgatattgt tgcctatggt gaactaagtt attcacctgc
    10081 acccactggt agttatccaa gagcaaatct gagcctgaat gtggcagctt atgctaatcc
    10141 tatccggctg attggattga ggatcatgga tccaacttct gatgcgaaac taggatggca
    10201 atttcatgaa gcggtatcca ttaatgttta tataccggcc ccaaccacaa taagtctttc
    10261 ctataattat tcaagtgctt caccaggtgg tggtttccct tcttttaacg gaaaccttac
    10321 aggaagtctt attagtgaat aggagcttat gataattata tattgaaaac tgttctttta
    10381 ttcattccgg cttaatctaa aaatcgttct ttcagatccg gcgtaggaat catacaacta
    10441 tcttttttgc caaaccattt ataccggttt ttggcgaccc aactataaat gccatcccgg
    10501 ataaatggtg gtacgataat aaaagcatat aataaagacc aggggccacc caattttttt
    10561 agcatgcgta aagcaccggt tgattttata taaatgttat ctccttctgc caggataaat
    10621 gaattaagct cacctgcagg taaactatgc tttgaaaata tttcctgtcc ttttttaccc
    10681 tgtaatggtg caaaaaggaa ttgttttttc ttatctcttt tgatgatgaa ctgcacactg
    10741 ctgttgcaaa gattacatac gccgtcaaaa agtacaattg aaatgtctgc catgctcggg
    10801 taaaggtaat ttcctttttc ctataataat tgtaatttac tctgctaatc tatttttaaa
    10861 agcaacaggt atgaaaagca ctttccttct ttttataacc atcgtgttta tccaatcctt
    10921 tatttatagc cagggaaaat atgccggctc caaaaaatca ctgattggcc aggtgtatac
    10981 tgattacctg gagttgcctg cttttaaagg atggacattt atggaaggtg gtgttgccaa
    11041 ttcacttagt gatccggaga tgattacttc caatgtattt aaaaaaggaa ccagttatgt
    11101 ggtttttttc agcatcagag aagataccgc ctccggccaa ttcactattg tggatgcact
    11161 ggagattaag ggtgtgcaaa agggctggac agtccggagc agtttttgca ggcaaaacaa
    11221 aatagagaat aattatatta tagcatgggg caaagaaacc actaatgagt atataaaatt
    11281 gataaaaaag gcatggcgat ttaacccgga taaacgaagg attgaaataa taccggtgaa
    11341 aggtattgat tgcatgaata tcgggtgtta gcaattacta actgattaaa accattaact
    11401 atgaaattga atagtaagag cccacaaaat cttttattga caggattact tttatttcag
    11461 acctcaatgc ttttttcaca gcaaccacca ttgccggcaa aaaaagttga accccgaaaa
    11521 gcaatcatcc caactaagcc aattgtaaaa aaagattctg taacctatgt ggccaccccg
    11581 gcccgggtag aggttaacac caggatagcg acagctgatg agatatatat taaccaggga
    11641 acttttttgt tgcgatccgg cagtgattac cgctatctta cgattaagga tgtagtacct
    11701 gcaacagggt cggttgcatt tttatggagt tttgtcaatt acccggttca gcaggaatgg
    11761 agttttattc ctaaacccgg tggtttctat aaaatcaaat catcctccgg tttttatctc
    11821 acacaaaaaa gattgtttgc tccttccatg gatccggagt cagccgagga tagccaatta
    11881 tggcagctaa tagaaaccgg tgatgggtat taccatatta aatcgaaaac caataaatat
    11941 cttgttgtca ctgacagaag gaaaagagat ggcggcctgg taagttttgc caacagtgta
    12001 accaatgatc tgaaacaaaa atggcatttg attaagcggg ataatgacag cagaaaacta
    12061 acatctttca accctgaaac aaatggattt cgttttgtaa atacgtttaa tggagaagat
    12121 atcatccggt ggggtgggtt gtgcggaggg atggtgtatt cagctttaga ttatttcagg
    12181 aacaggattc ccattcccac tcaaacctat actcctgcca atgcaactcc tcttcaatcc
    12241 tatatttatc agcggcagca acattccatg tggaatgtaa acgaaaagtg gtcagaactg
    12301 gaagtggcca ataatttcag ggcgggtgaa atattccgat ggggcattca gggtaccggg
    12361 ggaggaaggc tggaagaatt aaaaaatgca attgatgcag gaaaatcagt tcctatcgga
    12421 ttatttggtg ggggagtgcc cagtatcaat ggcggcagcg gaggtaatca tgttgtattg
    12481 gcagtaggtt attccctggg ccgttaccag ggagattttg gtaaccgtgc aggagaatat
    12541 aagattttag cctacgaccc caatatgcga aacaggatgg taacgctggt tccggatctt
    12601 gccgggcaat gtttttttga agtagaaaat ggaaaagcct ggagaaccta ttttgtcaat
    12661 acccgatatg acaatgaaca tacaccacca cgggatattc caaatttccc ggaaagagag
    12721 ccggatggaa gtatccgcca tttatacgtg gaatttgcaa caggcaatga tgacctgcgg
    12781 ggtggtaatg ataatgtcag tattaccgtg caatatgcag atggaaccac ccaggcattt
    12841 cctaatgtca ataatggggc aagatgggtg gataagagct ggcaaacgat tcacctggaa
    12901 ctaaacaggc cggtgcgaaa agctgatatc cgtcatttcc ggcttaacac cagttttgga
    12961 agcgatatat tcagtgatga ctggaatctt gaatggttca gggttacgac aggtttcggc
    13021 ggaattaata ttgcacaatc atttcctccc gaaggcagtg aatatatatt ccggttttca
    13081 ggggatgaaa gaaggcaaat gcatgtgatc agggtggagt aagacagagg agtcgatttt
    13141 attggaatat cattttgaat aatccgttat tttaaaaaga gtaactatga aatattttgg
    13201 attaatcatc gcaaaatttt tattgattgt gaatttcgtt atagcacaag acccgccccg
    13261 cagggatacg gcctggggcg attggcctga tcagccttac tataatggtg ttctttcagc
    13321 tccggtggtc gttggagaat ctgttgccga aataatatca ccagcaggac aaaaattatg
    13381 ttttgataag aaagtacgta tcaagtcaat gatcagcagc ggtgctgtag agcaatgtat
    13441 gtatatcaac actgtaaagg gctatgtagg aattcttccg cctcaacggg gaggtggcgg
    13501 accagggttg tgtgatataa agacggatga cgccaaattc ctgttttatg tactggggct
    13561 gaaagggaat gcatatacat tcaaaaatgc agaaaaaaac ggaactatcg agcattgggt
    13621 ctctacagga aatacacagg cacaccaggt taatcttaca agcccatcag ggaattacac
    13681 agtacataaa aaatctgaac ggagaggtta ttgcggggat aagataaaaa catgggctta
    13741 taaatacgat aatccatcca gtcctgttta tttccttttc ggaaaaactt ttccggaaga
    13801 gatcaatgta agcactaata aatacattgg aaatttcggg gtgggatacc agtttacaga
    13861 taaaggacta tatattatta tggaaatgga gagtggaagt tacagttgta aaataacaga
    13921 gctggaagag gtaagtgtat gtttcgatcc tgcacctttt aaaatagcgg aggatgaatt
    13981 ttttatgaag gcaagcgccg gcatacaaaa agaaagagat aagattgcca gagatgaggc
    14041 cagggtggga gatgattgtg tatcacaaaa aatggcagtt attgaattca gaaaggaaca
    14101 ggtaagattg caggaggcaa gactatatac cagccaacag ggtaatgtgt accaggaccc
    14161 cgctactcaa caagcaatgg cacagatgat ggatcatgga gcaatgacca aacaaatgat
    14221 cctggaaaac gaagtaaaaa tatgccaggc gcaaagacgg ctttcacaaa ccagtaacca
    14281 aagcagcagg gagagatacc agcagcgcat atcatgtctt ggaagccaga acgaccagtt
    14341 aagaagcctg gaggggcagc ttagagcaat agatattcaa tatgtaagtg agccgggcag
    14401 ggcttttgca gagaaatcaa aaaaatttat gcagggatta ccaagaacct gtgactgatg
    14461 aataggaaat aagggtttat catcccatgt tatgaaatac cttctgaaca tcatcatcct
    14521 gttctaattt ttcaatcagc ttacttacat cagctgcctg ctcgtcatta acaggaacgg
    14581 ttacgttagg tatccattcc agctcggcag aaataggagt gatgccttta tcttctaatg
    14641 ctttttgcat attaccaaaa tcggtaaagc cacaacgtac cactaaaata tcttcaccat
    14701 tttcaccagt gccttcaccg agttcttcca gtccgtaatc aatcagctcc agttccatgt
    14761 cttcagcatt caatccttcc ggctttagct taaacactcc cattttttta aactggaatg
    14821 acacactgcc actattacca agtgtaccat gtccttttgt aaaacatgac tttacattgg
    14881 ccaccgttct tacatggtta tcggtggctg tttctaccag tattgcaacg ccatgaggtc
    14941 cgtaaccttc gtacagtatc tcatcataat tcaccagttc ttttccctgt gctcttttga
    15001 ttgccgcttc tacccggtct tttggcatac ctacgctccg agcattctga aaacaacggc
    15061 gaagggctgc attggaattt ggatcggggc caccggcttt tactgcaatg atgatttctt
    15121 taccgatacg ggtaaagttt ttggccatct tatcccatcg ggcaaacatc gaggctttac
    15181 gtacttcaaa aattcttccc atgataaaaa gtcattaagt caaaagatta aacagatcaa
    15241 accgggctgc aaaagtaagg caataggggt aaaagaaaaa ccgcccaaag gcggtttgtt
    15301 ttatcagttc aggcaatttt attatttctt agttgactca aagtcatcag ctgtgggcag
    15361 catatctgca aaatttctca gtttacttct tttgcggcta taggcaaaat agacaaccag
    15421 cccaacagcc atccacataa atgctacttt caatgtctgg gtatcaagac caacgatcat
    15481 ggctatgcaa attatggcac ctaaactgca caccaccggc accattggag ttctgaacgg
    15541 acgttctata gttggatttt ttactcgtag tatccaaact ccaatactca ccaatacaaa
    15601 agcgaaaagg gtaccaaaac ttgtcagatc gccagccaca tgcccgggca caaaagcggc
    15661 aaaggcacca acaaatacaa acaagatcca gtttgcttta taaggtgttt tatattttgg
    15721 atgtaactga ccaaatgctt tagggatcaa accatcatta ctcatggtat agaagatacg
    15781 gctctgtccc atcagcatta ccaaaataac cgaggagaaa cctgccagga ttgccacggt
    15841 tacagcatcg gcaagccatt tgtaattcgt catatatgta ctgatagcat aggcaacaga
    15901 tgcctctctt cctttttctg ggtccacaaa atccttccag ggagctactc ccgttaatac
    15961 atatccgaat aagatataaa ggatagtaca taccactaat gaacccagga taccaattgg
    16021 catatctctt ttcgggttct ttgcttcctg tgctgctgta cttacggcgt caaaaccgat
    16081 aaaggcaaaa aatacaatgg ctgcaccacc caatatacca ccccaaccat gtttattcca
    16141 gctactgtaa tcagcaatta ttttacctgc ctggtctttg accgcctctg ttccttccgg
    16201 aatcatgtac ggggtatgat tttctggttt gatatattgc cagccaatgg caatgaagag
    16261 gatcactatc gccactttga taaatacaat aatagcattg acaaaagctg attcctgggt
    16321 gcctttgatc agtagcaagg tcagtgccag caaaatgatt aaagcaggtg catttaatat
    16381 accggagtga tggacaccgg cagagtcagt gaaactttca aatggtgaat ggcaccattc
    16441 atacggaata gcccctccca acagcttatt caggtattca ctccaggcaa tagatactgt
    16501 ggcagcgccc agggcatatt ccatgatcag cgcccatcca ataatccagg cgatcagttc
    16561 acccatcgtt acatacgaat aggcatacgc actgccggca atcgggatca ttgaggcaaa
    16621 ctctgcataa cacagacctg caaaagcaca accgatagct gcgataataa aggaaatggt
    16681 caccgctgga cctgctgcct gtccggcggc agccgcagtt cgtacaaaca atcctgctcc
    16741 aatgatagca ccaatgccaa gggcgatcag gtttccggct ccgagggtac gttttaatcc
    16801 tttttctgaa tctgcaacct ggatcaacat atgatccagc gacttctttc taaacaaact
    16861 catctaaaaa tctattttct tatttttaaa ttattttttc ccttttgatt cttttattgc
    16921 ttttagatat ctgtttagct cttctttaac cctgggaaaa aggaataaca gaccaatcat
    16981 gttagggaat accatcgcca ggatcatagc atcagaaaaa tcggtgactg ccgataatga
    17041 aacggatgcc cctatgacaa cgaatgccaa aaaaagaaat ttataactga ggtctgcgat
    17101 ttttccacgg ccaaaaagga atttccatgc ctgcaggcca taataagacc agctgatcat
    17161 ggtagagaag gcaaatagaa caatagcaat ggtaagtaca tatgaaaaat gcggtatcac
    17221 ttcgttataa gccatactgg taagatctac accactggtg cttcccccgg ttgcatttaa
    17281 aacaacactt ccttttccat caccaccata ggggaacatt aagccatctt tattgaacat
    17341 tataataaca agagctgtca tcgtacagat caccacggtg tcgatgaatg gttccagcag
    17401 ggcgacaatc ccttcagaag cggggtattt tgttttaact gcagagtgtg caatggatgc
    17461 tgatccggca cctgcttcat tggagaaagc cgctctttta aaaccaacaa ttaatacacc
    17521 catcagtccg ccgagcccgg ccctcggaga aaaggcttct ttaaatatca tggaaaaggc
    17581 atcatcaata aaactgaaat tggcaaatat gatgatcagt gaagcaacga tatagatgat
    17641 cgccattatc ggaacgatct tttcggttac tgttgctatc tttttaattc caccaataat
    17701 aacaatacct gttataatgg caagtgcaag gccaatgaaa aaacccgatg atgctccctg
    17761 cataccagtc atggcagcaa tttgttgcgc agcctggttg ctttggaaag cattaccacc
    17821 acccaatgaa ccgcccacac ataaaattgc aaacaggatg gcaaggaatt ttcccaatcc
    17881 ggccagtttt aattctttaa atcctttggt taaataatac atggggcctc cgtacactac
    17941 accattttta tcaatatcac ggtatttaac tcccaatgta cattcgacaa atttgctgct
    18001 cattcccagc aggccgcaga tgatcatcca gaaagtggca ccgggtccgc caattgcaat
    18061 ggcaatggct acgccggcaa tatttcccaa tcctaccgtg ccggagacag ctgttgctaa
    18121 cgcctggaaa tggttaacct caccatgatg cgattcgtct ttgatggttt ttacaatatc
    18181 gccatcaacg acatttacat ttggatcttt cacattgttc gcatcttttt caatgtcatc
    18241 atgcttgcct cttactactt cgatagcgag accgaatcgc cggatattta caaaagaaaa
    18301 atagagagtg aagaataaag caccacaaac aagtagtatc acaacaaggg gtacctgaac
    18361 accgcccaga ttaattgtat aaaatacaaa actgctccat gcatccgtaa tgggctgaaa
    18421 ccactcatta atcctttcat ccagtccttt gggcggagct ggcgtatcct gggcagagga
    18481 cagaaaaggg acggcaattg cagtcatcat catataaaaa atcttcgata tgttttttag
    18541 catacagcgt tggttttgat cgaaatggta attaaagtgc gtaaagtaat taaatattta
    18601 gcagaaagat tgaaaaatat ccaccaaagg tgcttattca gggtcgcacg gatttttaag
    18661 taatttcaaa gtaaaccttt agttttgacg ccttactata attaatctat gaacagacga
    18721 aaggccggcc tgctctccct cctgttattt acggtagtag cacttattca tttcctgcac
    18781 atgcagaaat ggatcatact ccaggaagtg gtattaatga ctgttcgatg gatatttatt
    18841 gccagcctgg tattatttgc tgtttataag aaatcattga ccacatggat cttagtggcc
    18901 atggcgatag gggtagaaat aggactcgat tttcctgctt tctcacaaaa ccttcaattc
    18961 ctcagcaaga tatttctccg gttggttaaa acgatcgttg ctcctttatt atttgccacc
    19021 cttgttgtgg gaattgccag tcatgctaac ctgaaacagg ttggccgcat gggttggaaa
    19081 tcattgttat attttgaagt ggtgactacc attgcccttc taataggcct ggtgtttatt
    19141 aatattacaa aagcaggtat cggtattaag gttccggagg ctttattaaa agaattgccg
    19201 gaagtaaaag tgaaaggatg gcaggaccat ataatagata ttttcccgga gaatattata
    19261 aaatctatct acgagggaaa tgtgctgcct attgttttgt tcagtgttgt ttttggtatt
    19321 gctctcgcct tgttaaatga gcagaagaaa aagccaatgc tgaattttgc agaaagcctg
    19381 gcagaaacca tgttcaggtt caccaatatc atcatgtatt ttgctccttt tggggtaggt
    19441 gcagcaatcg ctgttactgt cggtcactta ggtattgaca tattgaaaaa cctggcttta
    19501 ttacttgtta cactttattt agcattgatt gcttttctta tcctggtttt attaccgatt
    19561 gcaatatggg tggcaaaaat tccgattaag aaatttatac aggcgatacg ggagccggta
    19621 tcgatcgcat ttgccactac cagttcagat tctgctctgc ccaaggcttt ggagaatatg
    19681 gagaaatttg gggtgccccg gaaaattgta tcatttgtga ttcctacagg ttacacgttt
    19741 aacctggatg gaactacttt atatctttca ttggcatctg tttttgttgc ccaggcagca
    19801 gggatggatt tgtcatttgg agagcagtta atgatcggtc ttacattgat gttaaccagt
    19861 aaaggtgttg ctgcagtgcc cagggcatct ttggtcatct tgattgcgac ggcaacaact
    19921 tttcattttc cactatggcc tattatggcg atctatggta tagatgaact gatggatatg
    19981 gcaaggactt ctgtgaatgt gatcgggaac acactggcta gttgtgtgat agcaaggtgg
    20041 gagggtgaat tcgatgatga taaagccctt gcttttaatg agcaggaagc cgatgctatc
    20101 agggattaat gcggctggca tctgtccggt ataactaacc cttagcaaag tctgcagtct
    20161 ttaagtgcag gctgcacttc aatggtgggt ttataatcta cttttcagtt ttaatttccc
    20221 tggttattta ttcttccgcg actttatgtt taatgattca tggtgcaata aaaatgggat
    20281 tcctgaattc gtgtcatggt tttcggttca tcgtaaaaat gtcctatttt gccacatcgt
    20341 atcattaata aagcccttcc ggcagataca tgaaaaaggt ccctttgtat atcaagatca
    20401 ttgcgggaat gttagcaggt gtattatttg caattgttgc tattcaattt agcggcggtg
    20461 taaagtttac tagagactgg atcaagcctt ttggtaacat atttatcaat atgcttaaac
    20521 tgattgcagt tcctttgata ctggcctccc tgataaaagg ggtgtctgat ttaaaagaca
    20581 tttctaagtt atcaaggatg gggttaagat cagttgtact ttatcttttt acaaccgtat
    20641 tcgccgtttg cctgggactg gtgatcgtca atattatcaa acccggtgca tccatcagtg
    20701 aagaaacgag gacccagatg ttatctgctt atggtggtga tgccaataaa aaacaacagg
    20761 aagcggttca gcaacagggc tcagggccgc ttaagttcct ggaagatatc gttccggata
    20821 atatcattaa tgccgcttct acgaactcga ccatgctgca ggtaatattt tttgcgttgt
    20881 ttttaggcat atcattgatt ttgataccgg cagaaagagc aaaaccagta aaagattttt
    20941 ttgacagttt taatgaggtc attctcaaaa tgattgacct tattatgaaa atcgcaccat
    21001 tcggggtctt tgcattgatg gcggccctgg tagcggaatc tcccggtttc gaaatattta
    21061 aagcgctggg taagtatgcg ttaacagttc ttattgggtt agtgatactg atctttattt
    21121 tttacccggt tctgatcttt ttatttacca aacggaatcc cattcagttt attaaacagg
    21181 tttatcctgc acaattgctt gctttttcta ccagctcaag tgctgccacg ctgccggtta
    21241 caatggaaac agtggaaaaa agagtgggtg tcgacaatga agtcagcagt tttgtgcttc
    21301 cgattggggc tactgtaaat atggatggta ccagtttata ccaggccgtt gcggccgttt
    21361 tcattgcaca ggcttttggt atggatttag gacttaccgc tcaattaggg atcattgtta
    21421 ctgccaccct tgcttccatc ggatctgcag cagttcctag tgcgggtatg gtcatgctgg
    21481 tgatcgtcct ggcgcaggcc ggaattccgg aagcaggatt agcacttata tttgccgtag
    21541 acagaccttt ggatatggta agaacagtag ccaatgtgac cagtgattgt acggtggcta
    21601 ctattgttgc aaaatcaatt ggaaaattag aagatcctca ccctgttgtt taacacattt
    21661 atttaattta ttatgttcga attattagat gcctttcatt ggacccagct tttacaacct
    21721 cagttttatg tagaaaacgg gggactatgg ttgttgttat ttgttgtttt tgccgagacg
    21781 ggattatttg ttgggttttt cctccccggg gattctttat tattcgtagc cggtatttat
    21841 gcccatgaaa taacccaaaa aacaggtgaa gtggttcccg gcctggctta ccagtttctc
    21901 gatcttattg gaatgggaca tatcagaaat gaatgggtag atctgtttgt actggtggga
    21961 cttattgcat tcatgggcat actcggcaat acagtagggt attggacagg ccgaaaagtg
    22021 gggcccgcaa tgtatcaatg gcgggaccgg tttctcttta aaaaacgtta cctgcaccag
    22081 gcgcatgatt tttatgaaaa gcatggaggg cttgctgtaa ttggtgcccg tttccttcct
    22141 tttatcagaa cctttgcgcc cattgtggca ggtatagtac aaatggacag aaagaagttt
    22201 cattttttta atatagtggg tagcatcgcc tgggtattct ctatgatttt tggtggatat
    22261 tttttgcaaa aatggatact aagccagttt ggctttgact taaaagaaca cctggaattg
    22321 atcgtgctgg gtatcgtgct ggtcaccacg gcaccggtaa ttattaaaat gatctcagga
    22381 cgcaaaaaga agcctgctga aacaacaggc ggtgattcaa aacaatcata aagcatgaac
    22441 caggcaaaac ctgtttccat ttttaatatt gctgttattg ttgcagcact tggatacttt
    22501 gtagacatct atgatctgtt gttgttcact attgtacggg aacccagtct tgcgggtttg
    22561 ggtgttgacc taacaattaa aaaagatgta atagctgcaa gcaccaaagt aattaactgg
    22621 caaatgtggg gcttgttgat cggtggtatt atctggggag tgatgggaga taaaaaagga
    22681 aggctgagtg tattgtttgg ttctattatt ctttattcta tcgccaattt ttttaccggg
    22741 tttgttcaaa cagtagagca gtatgctttt gcaagatttg cagcaggaat cggccttgca
    22801 ggtgaattag gtgccggtat cacattggta agtgaattgt tgccaaaaaa taagaggggg
    22861 atcggaactt cattagtggc tggtattggt ttgtttggtg ctgtagccgc ctatttcact
    22921 tttaaattta ccgatgattg gcggttgtgt tataaaatcg gagcagggtt aggaatcatg
    22981 ttattatttc tgcggattaa agtagcggaa agcggaatgt tcagccaggt gaagaatcag
    23041 aatatttcaa agggaaacct gttgatgttt tttaataacg gaaagcggtt caagaagtat
    23101 atacttgcaa tactcatcgg tatgcccacc tggtttgtta ttggcatttt agttaatctc
    23161 agtaacaggt ttgccactga gttttacggg gagaataaaa ttgagtcggg ccgtgctatt
    23221 atgtatgctt atgctgcaat tgctatcggt gatatattgg ttggattggt gagccagtat
    23281 tttaagagca ggaaaaaagc gttatacctg ttttatttct ttgcaattgt ttccggaatt
    23341 tactttttta gcgggtatgt tcagagtgat acatcgatgt atatagcatg tgctgctttg
    23401 gggttcagta ccggattttg ggccatattt gttaccatgg gtgcagagca gttcggtact
    23461 aatcttcggg caacagcagc tactacaata cctaattttg taagaggttc tttaccgctg
    23521 attaatttaa tgtttgttgg tttgtttcag caaagtattg gctggagttt tgtgaaaagc
    23581 gcaatgatta caggcataat tgtaatgttg ataacactta ttgctgctta ctttacagaa
    23641 gaaacgttcc ataaggatct gaattatact gagcctgaag atataatgcc ttaacgtatt
    23701 gaatgtggca aaattccttc tcatacggtt ttcatcgatt ggtgatattg ttctcacaac
    23761 acccgttatc cgctgcctga aaaaacaggt gccggatgca gaggtccatt ttttggtgaa
    23821 agattccttt cgttcagtgg ttgaacataa tccttacatt gataaagttc atgtactggc
    23881 acatagctgg gaactgatga tggaagaact aaaaacggag gattatgatt atatcattga
    23941 ccttcatcac aataccaaaa cgttgagggt taaaaatgca ttgaagaaga aatcatattc
    24001 attttataaa ctcaatattg aaaagtatat tcttaccagt atcaagatta atattttacc
    24061 acgggtacat atcgtcgacc gttacattaa aactgttgaa tcatttggag tgaaaaatga
    24121 tgggctggga cttgattatt ttatttcacc tgccgatgag accagtaaag aagatatccc
    24181 ggtttcgcat tcagccggct atatagcctg cgtgattggg gcggcacatg gtactaaaag
    24241 atggccggta cataagtgga tggcattttg taaagaaatt gaccatcctg ttattttgct
    24301 gggaggaaaa gaagacgctt ctaacggaga tgaaattacc tcggttgacc ccataaagat
    24361 ttataatgcc tgcggtaagt ttaagcttaa cgaaagtgcg gacctggttc ggaaagcaag
    24421 actggtcatc agcaatgata caggattgat gcatatcgct gcagcttata aaaagcaggt
    24481 tatttctttg tgggggaata ctgttccgga atttggcatg acaccttatt acggagatag
    24541 tgccatttcg tctattccat tacaaacaaa agtctggtgc agaccttgtt caaagattgg
    24601 ctatgacaaa tgcccgctgg gccatttcaa atgcatggaa aaaattacag tagatgaact
    24661 cttggaaaga gtcaggcaaa agattgcact catttagggc aagaatacct ttgcctggct
    24721 ctaaagaata tacaagtgtg ttttcggctg tttactaatt cataaagaat acagccagta
    24781 aagcaaagac taccaaaaag cctgtaataa tcaaacgggc ctgtacgtct tcattgctga
    24841 tcatgtgtct aattttcttc ataacctgat tttttagttt tcaaaacgaa tgttaaccag
    24901 gttttccagg agttgggata aggtattcag aaaggatagg gggacaatga cttagatggt
    24961 acaaaaatag aatgcaaaat cttgaattgc aagtataggc cggtattttt ttaagttaga
    25021 tttattaatc ggaaaactac gatgtttcgg atctgatatt gtatttaaga tgataattat
    25081 aataagcaaa ttgtgattta tactactatt tatatttttt tcctggttgc ctgttcattg
    25141 gatgctgctt cccggcaacc tgtaagccat atcgattcat tcagattgta tggcgatgta
    25201 aaggttacaa tagataagcc tgctgatttt aaaaaaaata aagaaactat tattactttt
    25261 tttgcgcttc ctaatggtaa tactacagaa caaacaatgg gtaaaagaat gttgccgggc
    25321 gatgacttgc attttgatat tcaacacata agggcacaga caaagtttat acgccaacaa
    25381 atgaagaaaa agaactttgt tgtcatttac ctggagaata attataaaag ctggcctgca
    25441 tggaaacaga agcatcccgg gtacagacat gaaatacctc atcttataga ttccctcgct
    25501 ggtctgtcaa aatcaagaaa aagaagtgta tacctgaatg ggcatagtgg gggaggcagt
    25561 ttcatctttg gttacctggc tggtgtggaa cgtattccgg ctactataaa acgaattagc
    25621 tttcttgaca gtgattatgc atatgacagc agctattatc ctaaacttaa aacctggttg
    25681 cagcaggtac ccggaagttg ccttaacgtt tttgcttaca atgacagtgt cgcaatatat
    25741 aatggtaaac ctgttgtgtc tccgaccggg ggaacatggt acagaagcag gttgttatta
    25801 agtcatttgc gaaacgattt tatattttca gaattaatta cagacagtct tattgtaaat
    25861 aagagcaggg atcacaggat acagttttac tttaaagtaa atcctaacca gggtatttat
    25921 catacgcagc aagtggaact gaatggcttt atacattcag ttctttgcgg aacccggtat
    25981 gattcacaag ggtaccgcta ctattccgga agggcttatg ctgacctgat cgaataagtt
    26041 ctgttgtcag tagctggttt gaaataaaac cgggaactgc ttttttaatt tttcctgttg
    26101 tttggaactg agattctcgt ataattttct atgtttttgc aggcacaatt cagttaaggc
    26161 ccttttctga ccttctttta tttcattgat aacagcatca gttaccaggt caggaatatg
    26221 ggggtcggtc ttcactaatt ttattttatg gtgcattagc ccggtaccca tataactttt
    26281 aaataggcgc tcctgtatat accgcgccag ttcatcggag ccaaccctat cattgccaac
    26341 ggtgatcagt ccggtttccc ggagagtgat caggagtatt ttttctgctt tgaattctct
    26401 aatcggactt gcatctgtct ggaatatcag acaagtaagg cataacataa aaaggaaacc
    26461 gcttcgaagc ataatacaat ctttaaatac gcagcaagga tttggtgtaa gtacaaatga
    26521 agctatgttt ttatataatt ctaccaatat ttaaactgaa aattttaatt attggatttc
    26581 actaagttcc agccagcgaa gttctttttc atctaaaagc cttgttacat cgccaatacg
    26641 gtttgaaagt tgctgtagtt ccccgaatgg ggtattgcca ttattcagtt tttctgtaat
    26701 ggatattttt tcttttgaaa gatcgctgat ttctttttcc agcaattcga attccctttt
    26761 ctctttataa gagagttgac gttttttccc ggcagttacc tggtcctggt tagcagaaga
    26821 aacgggtttt tctatttcgc ctatatagga agcaccattt ttttctgtta atgactggcc
    26881 attggctgta gcctgcctgt attgtgtata gttaccagga aaatctctta tcatcccatc
    26941 accttcaaac acaaacaggt gatcaaccag ccggtccata aaataacggt cgtgagatac
    27001 gataagtaaa caaccgggaa attcacttaa gaaattttcc agaacgccca gggtaggtaa
    27061 gtcaaggtcg ttagttggtt catcaaggat caggaagttg ggattccgga aaagaataga
    27121 aagcaggtgc agtcttcttt tttcaccccc gcttagttta cttatgtagg tatattgttt
    27181 atccggatcg aataaaaata attgcaggaa ttgcgctgca ctcagtgaac caccggtagc
    27241 caggggaaag ttttcggcaa tattttttac gaattcaatc acccttatgt cttcttttac
    27301 aatcaaacct tgctgggaat aattaccaaa aatgacggta tcgccaatat tgattttacc
    27361 actgtctgct ttttcaagtc cctgcaggat attgatgaaa gtggatttac ctactccgtt
    27421 ttttccaacc acaccaatcc gctctccttt tttaaaagtg taatcaaagc ctttcaggag
    27481 tatcttgtca ccataacttt tatatatttt tttaagctca ataatttttc cccccaaccg
    27541 gctcatcttc atatttaact gcaactgttc atcaatgatc tgctgcttgg cttttttttc
    27601 tacttcataa aaattatcct gccggctttt actttttgtt gttcttgctt tgggttgctt
    27661 ccgcatccac tccagttctt ttcgataggt atttttggct ttatcaatgg aggagagatc
    27721 attttctatc cgagcagctt ttttttccag gtaattctcg taatcccctt tgtatacata
    27781 catattgctt ccgtccaatt cccatatttc tttgcagacc gtatccagga aatagcggtc
    27841 atgggttact aaaagcaggg tcactttttc tttatcgagg taatgctcca gccattctac
    27901 catttccaca tcaaggtggt tggtaggttc atccatgatc aacaggacat gtttatgctc
    27961 aaagccaata tcaatcagtg tttttgccaa tgctactctt tttcgctggc caccacttaa
    28021 ggtactcaca ggttggttca ggtgatgaat attcagcttc tcaagcacct gttttacttt
    28081 ggtatcaaaa tcccaggctc cgagttcatc cattttgatg atagcagcac ttagtttttc
    28141 cgcattttcc tcttcgctaa tgacttcata ttctttaatg gcgttaatga tgggatggtt
    28201 atgaaagaat atattgtcca gcaccaactt ttcttcaacg aaagtgggtt cctgttcaaa
    28261 taacactaca tcaacatctt tattgaccca tacttttcct tcatcagctg tttctttgcc
    28321 tgccaatatt cttaataatg tggatttgcc ggtgccgttt cgggctacca gggcaatttt
    28381 gtcgccttct tcaatatgga atgttatatc tgaaaagagt ggttgtatgc cataactctt
    28441 ggtcaatcct tcaactgaaa cataatgcat agcggcaaag ataacttaac gtcccagaag
    28501 atagataaac agcgaaataa tgattgtcag gataataatg acaaccaggg aatatttttc
    28561 ccggttattc tttttttgac gttgccggat gaaaatattt agcttctctg ttgcgatagg
    28621 ctcatttgca ctcagtttgt gaagttgtat aaaaatgtct ttggtgtatt gccctggtaa
    28681 tggttttatg gcatgtaata aaattgaaac aatttgccgg attgtatccg gctcattaaa
    28741 ttgttgaagt ttttcgatcg atgtatcagg tattaaatcc atgatatact gttgtaatcc
    28801 cattttatcc attctgaaaa catcaatctt tgaaacatat tgctccagct caagggcttg
    28861 tttaagtact gttacgggag tgattacttc ctgggttttt ctgttgccgg tactttgatg
    28921 gtaccaacgc tgttgcaggt aatattcctt tttggccgga tcagtcagca tttcatacgc
    28981 ctcttttata tcggcaaaca gcgctgtagc atagggatca ttgttattct tatccgggtg
    29041 atattgcttg gccagtttcc ggtaagcctt ctttacctct ggtaaagaag cagaaggctc
    29101 cagctcaagt atttgataat aatctttcag atacatagaa aacaacgaag ttaagatggc
    29161 tgctgttcac aactaaacct accttttaga aagaatttac aaccaactaa catggaaacc
    29221 ggtcatataa agacaaataa gtttaatttt aattatcatt ttatatttta atttatatat
    29281 atggatacac ctaacaaaaa aaaccgcaga gagttcttga aaacaaccgg taaagccggc
    29341 ctggctgctg gtttgtctgc caccatatta ccggtcctgg cctccgttca aaccacgcag
    29401 ttcatagctg ctggcggggc tgaagatgtt ttatacatcc agcgaccttt gccctatgct
    29461 tttaaaacac tggaacctgt tattgatgcc atgactatgc agattcatta tatgaagcat
    29521 gctgctgcct atgccaataa cctggcagat gcagttaaag cggagggggt ggatacggaa
    29581 aaagagcaat tacgattcct gcttggacgc atttccaaat tttcgtctaa aatgagaaac
    29641 aacggtgggg gtcattataa tcatgaattt ttctggaagg gaatgacaag cccggtagaa
    29701 tcgaacaatc cttcgcctgg cttgctgaaa gatataacaa gggattttgg ttcttttgaa
    29761 gcttttaaaa cgcagttttc tgatgcagcc aaaaatcgtt ttggtagtgg ttgggcctgg
    29821 ttagtcaaaa cagctgataa gaagttagtg gttggatcca ctcccaacca ggataatccc
    29881 ctgatggatg tgagtgacct gaaaggaatt ccattattag gcctcgacgt ttgggaacat
    29941 gcttattacc tgaagtacca aaaccgtcgt ccggattaca ttaatgcatg gtggagtact
    30001 gtcaactgga agatcatcca gcaacgctat gaaattgctg aaatgaaata atcattgcag
    30061 gtatttgtta aagaattcaa tagtccgctg ccacgctagt ttggcagcgg tttcattata
    30121 cctggtgggt gccgtatcat tatgaaaggc atgctgggcc ccctcgtaaa tatatagttc
    30181 gtaagggatg ttgttttttt tcagagcttc ttcataagcc gggatacctt tattgactcc
    30241 ttcatccaat gcgccataat gtaattgtac cgctgctttt attttgctca cctcttctgc
    30301 aaccggttga cggccataaa aagcaacggc agctttcaat gccggaacat tgacagccag
    30361 gttattgctc atagcaccgc cccagcaaaa tcccacacat ccaaactgac cattacaatc
    30421 ttttcttgct gacaggtagt cgaaaaccct gataaaattt tgcaggttat cttccggttt
    30481 taattgctga aattttgttc ttgcttcatc tgcattttcg ggagtacccc ctaaggatga
    30541 caacgcatta ggtgcaatag ccagaaaacc ggctttggca gcacggctgg ttacgtcttc
    30601 aatatgggca tttaaaccac ggttttcatg aatgactaag accgctgcat attttttttc
    30661 ttcttttggc cgggcaacat aagcctgcat atcactatta ataccctggt agctgattcg
    30721 ttcaataagc aggtcatctt gtggggtaac agctgcatgg gcataattta cttcgagtaa
    30781 aggtagcaaa gccatagccg cagctgtact gccggccagc atagcgagcc tgcgtaaaaa
    30841 ttcctggcgg ttaagtggct tatgagtgta ctcatcgtag aggttaatga tttgctggtc
    30901 catgttcttt aattaagatt ttaaaattaa ggaaatagaa gcagtaatgc ctacggctgt
    30961 ttctaacagt atttatgctt aggtaatgaa gttggtcttt ttgaatctct ttcaaaacgg
    31021 aagtatgtaa atggttgcta ctgctttaac acatatacaa tgttttgcta aaatggcata
    31081 ttaatacgta ttcagttata gtcatggcat aaaaatagcg tcatagttag tgtaacaaga
    31141 aagaaagcca atcaacaatt tcagtatgaa tctatttact actaaaaacg aaagacgatg
    31201 aaacgaatca tgaaaattgc tgcactggca tttgtattgg tgctgggcag ttcgcttcga
    31261 caaaccgcct cggctcaata ttatgatggc cagcgagaag tttcttacca gacattttat
    31321 gacgaactga gtccctatgg ccgttggatt gattacccgg gacaaggtta tgtgtgggtg
    31381 ccggatgccg gtcctgattt ccgtccgtac agtaccaacg gacattgggt gtggtcagaa
    31441 gatgctgaat ggatgtgggt atctgatttt gactggggct gggctccttt tcactatgga
    31501 cgctgggata atgatccctt ctatggatgg tattgggtgc ccggctatga atggtcccct
    31561 gcatgggtag cctggagaga tggtggtgat tactacggct gggctcctat aagacctgga
    31621 attaacatca atattaattt caatatcggt ggttattcgc cgccttacga tttctgggta
    31681 tttgcaccac gacgttatat cacttctccc cgcatctttg attattatgt tgaccgaagg
    31741 agaaatgtga ccatcattca ccagaccgtg atcattaata attttaatta tggtggtaac
    31801 tatggttatc gtacaggccc gcgccgctat gatgcagaaa gatattgtgg cagaattgaa
    31861 ccggttcgtt tccgcgattc atacagaccg ggcagaacac agttcaggaa taatgaagta
    31921 agcttttatc gccctaatat ccgtcgtgat gataacagaa gcattagtcc ccgtcagttt
    31981 gaacggtatg acaggaacaa tgctggtgtt cgaaacggaa ataatgataa cagcttcagg
    32041 ggtaacggta atgatatagc tcgtaatgac aggagtaacc ggcctgataa ccgtgacagg
    32101 gccgacaatt cccctttcag aagaaacgat aattctgtaa ggcaaccaca gagtaatggt
    32161 ggcaataatc ttcctgaccg ccggaggatt gacagaactg ataataacag cagtcagcca
    32221 aaccgggaag gtaacacaag agttatcagg cctactgaac ggcctgacag aaattctaat
    32281 ccgggaaata ataacaatga gcgtcgtcag tttgagcgca gaaatgataa tggcaatacg
    32341 aattcacctg ttcgccagcc agaagtcaga aataatgttc cgcaaagaca ggcaccccgg
    32401 gtagagcaaa gaagatcaca ggaacaaccc cgccaatttg aaagacggaa tgatataagc
    32461 agccgccagc cggaagcaag aaataataat agcccgcaaa ggcagacccc ccagtttgat
    32521 caaagaagaa cacaggaaca acctcgtcag tttgagagaa gaaatgagag taataacagg
    32581 caagagagaa atagtaacaa taacggaaac agcagtagcc gaaaccgcag ggggtaacat
    32641 agtctggaaa ataaaaagag aggctgttat taataacagc ctctcttttt atttataatc
    32701 aaatcaagct tccgcaacca tatagctctc taccggttca caagtacaaa tcaggtgtct
    32761 gtccccatgg gtattgttca cccgtgctac ggaaggccag aatttattta gtgtaacgta
    32821 atgaagcggg aacgctgctt cctgtcttga gtaagcatgt ttccattcat cagctgtaac
    32881 aacaaattga gtatgtggtg catttttcaa tgcattatcc tttttgtcgg ctcttccttc
    32941 ttcaatggct gtaatttctt cacgaattga aattaaagca tcacagaaac ggtccagttc
    33001 agctttgtct tcactttcgg ttggttctat cataattgta cccggtacag gaaaactcat
    33061 tgttggggca tgaaaaccgt aatcaatcaa ccgctttgcc acatcctccg cttccacttc
    33121 ggctgatttc ttgaatggcc tcaggtcaac aataaattca tgggcgcatt gtccgttatt
    33181 gttagtgtac agaatatcat atttgccatc caacctggcc cgcatgtagt tagcattcaa
    33241 aatagcatat tcagttgccg ctttcacccc ttcattgccc aacattcgta tataaccata
    33301 gctgataagc agtatggaag cagaaccata cggtgctgca gaaactgcat ggctgcttcc
    33361 attggccaca tgaccgggaa gatgttttgc caggtgggat tttacacaaa ttggtcccat
    33421 accggggcca ccaccaccat gtggaatggc aaatgtttta tgtaagttca ggtgacaaac
    33481 atcggccccg ataagacccg gagcagttaa tcctacctgt gcattcatgt ttgcaccatc
    33541 catatacact tgtccgccgt gctggtgaat gatttcagtg atctctttca cggtttcttc
    33601 ataaacacca taggtgcttg ggtatgtgat cattataccc gccaggtctt tactgtactg
    33661 atcagctttt gctttcagat cttccacatc gatataaccg ttttccaatg ctttaaccac
    33721 tactactttc atacccgcca tcactgctga tgccgggttt gtgccatgtg cagaaatcgg
    33781 gatcagcatt actttgcgat gatgatcgcc attcgcttca tggtatgatt tgatagtcag
    33841 caaacctgca tattctccct gtgctccact gttcggttgt aaactacagg catcaaaagc
    33901 tgttatttca cacaggtaat tgctgagttc atcaataata tatttatatc cttctgcctg
    33961 gctggccgga gcaaaaggat ggatcttgct ccaatgaggc cagcttaacg gcatcatttc
    34021 agaagctgca ttaagcttca tggtgcaact tccaagagag atcattgaag tattcaatga
    34081 aagatcttta ttttccagtt gctttatata acgcatcatc tggctttcac tcctgtacgt
    34141 gttgaaaaca gggtgcgata agaacggtga tgttctggta agtgatgaag ggatatgctg
    34201 caattcagca tcctggtcaa tatcaaacgc taccggattc ttgtcatcct cgaagcaatt
    34261 gataaggtcg tagaggtctg ttacgtctgt agtttcgtca attgaaattc caatatgatt
    34321 attatcaata taacgcaggt tgatcagttg cctttccgct ttatctctta ttgtttttaa
    34381 cgtatcggtt tttacaacaa tggtgtcaaa gtaagtatca ctaagcagct caaatcctct
    34441 gtcttctatt gcttcagcaa cagtttgcgt aaagagggct attctttttg caatattctt
    34501 taatccatct ggtccatggt aaacagcata catagcggcc atattagcca ataaagcctg
    34561 tgcagtgcaa atgttagaag tggctttttc tcttttgata tgttgctctc ttgtttgtaa
    34621 agccatacgc aaagccctgt taccatatgc atcaatacta attccgatga tacggccagg
    34681 gatgcttctt ttaaaatcat ctttggcggc aaaaaaagct gcatgcggac cgccatatcc
    34741 caacggaaca ccgaaacgtt gcgctgaacc tatggcaaca tctgcatcca attcaccggg
    34801 aggagtcaat aaagtcaatg ccatcaggtc agtcgccatg acgacaaaag ccccggttgc
    34861 atgaacttta ttaatgaaat cacgataatc ttctatagag cctttttcat tggggtattg
    34921 gacgattgct ccgaaatagg tttcatcaat aacgttattt cgatagtcac cttccacaat
    34981 ttcaatccca accgggactg ctcttgttct caacaggtct tttgtttgcg ggaatattgc
    35041 tgcgtcaaca aagaatttcg gtctttctat atggtcttgt ttgttcagtg tgttaaagaa
    35101 catagtcatc gcttcggcag cagcagtgcc ttcgtccagc aaggaggcat tggctattgg
    35161 taatgcggtt aaatcactta ccattgtttg aaagttcaac agactttcca ggcggccctg
    35221 tgaaatctct gcctggtagg gagtatactg ggtataccat cccggatttt caaaaatatt
    35281 ccgcaggatg acggaagggg taatagtatc gtaataaccc tgccctatat aattagcaaa
    35341 tactttattt cttaacgaca cttccttaat atgcttaagg tattcatgtt cactcatggc
    35401 ggcaggaata ctcaacgggt tggtcatccg gatgccggca ggaactgttt tatctattaa
    35461 ggctgataaa ctggcttcac caatcgtttt caacatggct tttgtgtcag cttcattggg
    35521 tccgatgtgg cggggaatga attcgttaga ttgttgctcg agaagactca taaaaggata
    35581 cttataattg taaaaggatg tgcaaagtta cgggaggcca gccagaaaaa aggtgctttg
    35641 ttgaaggtgg ggataagcgg tgaaatgcct ttctttgcct gatgcaggaa aaattattac
    35701 agaactggga aaagaaatcc ggcgaacgcc aaaaagagta taaaaagttt ctgaaccggg
    35761 ccgataagaa taaagtattg aaacagttgc ctttgctgaa cgaggaagca ttcagtaaaa
    35821 ttgattgcct gcaatgtgcc aattgttgca aaaattattc tccccggttt acaccacccg
    35881 atataaaacg tatcagtaag tacctgggta tgaaggaggg tgattttaaa gacacttacc
    35941 tgctcaccga cagcgatggt gataatgttg taaaagcatt gccctgccca ttcctgggta
    36001 ctgataatta ctgcagtatt tatgaggtgc gcccgaatga ttgtgcccgt ttcccctaca
    36061 tggatgatga ttttatgctg aaacgccagc ctttggcatt gaagaactcc accttttgtc
    36121 cgattaccta ttacgttatt gagaaattga tagcagccgg taaatagtaa gatagcggct
    36181 gattgatatc tatacggggc gaataaaata tatgacaaaa tggattaaat atatccaata
    36241 tgtcgtatat ttgataaaat tggaaaaatt gaaaaaagca gtagttatta aaaaatcaaa
    36301 ggcttaccca aagccggaat ttgataatcc ccctgcatta caggaggctg ctgttaaata
    36361 tgaatcactg cgggtaattt taggaggccg gcaaactctc cggcaatctt tatccggaga
    36421 ttttgattta atcgccctta cacgggaagg cattaaaaaa tctactttaa agtctttggc
    36481 agaacacctg ggtatatcca tggaaactat gagtggtctg ctgcatagct cataccggaa
    36541 tattcagcgc aaggatgaag atgaattact tgatacactg aaaactgaaa aagtactgga
    36601 attggctgct tttgcacaaa gaggtattga agtcattggt tccaaagaag cttttaagga
    36661 atggttgcat agcccaatcg tggcattagg aaataaaccc cctcttgatt ttttagatac
    36721 ttcttttggt atccagttag tcattaagat tttaggtcgc ctggaacaag gcgttttttc
    36781 ataatgtatg gaattgtatc gtatcacaca ggaaatatat gcaggcgatt taagcggtaa
    36841 tggtgctaag ttgttcggtg ggcgctggaa tagcgaagga ttgccggctc tctatgcagc
    36901 ttccacccgt tcactggctt tactggaaac actggcacat acaccagcca aaatgctcca
    36961 ggtaaaagtg tatatgcttt taaccgttat tgtcccggat acaattttac cactggttat
    37021 tgagctcagg aaattgccgg acggctggga tgttccggat atccgtccat ttactcaaaa
    37081 gacgggagat agttttttat tggccaaaaa gaaccttctt attgctgttc ccagtgtact
    37141 actgccggaa gaaaataatt ttgtattaaa ccctttgcat ccagatgcaa aaaaaataaa
    37201 aattgttcac agcaggagaa ttcattttga caagcgggta gtggtgaatt tgtaaaagtt
    37261 tttataattc cgggaattat aatgaaaggc tattactgtt agaaggcttt attaatttat
    37321 tccccacctc catcaccgcc gctttctcca ccactttccc ctgggtcgct ttcagtacta
    37381 ttataatcag catcggggtg atctttatga cccgtttctg ctgctgtacc tgcatcactg
    37441 ttattatcac cagaatcttt ttgttggttc tttttagaac cagaagacga gacgataatt
    37501 ccccagatga tcagggcagc tataatgatg acaattattt tcattttcat tatttaacag
    37561 tcaactcttc ccaggctgcc gtcagaggtc gctgtatgcc gtctcctttc cattggtcag
    37621 aaccggggat ggcagaagcc tttagtattt cttcttttgt cttaccggct ttaatttctc
    37681 cttccacaaa ttttaatacg ttgccgagat aatcccggaa cactttcaga tcatcggctt
    37741 taccagttac ttcatagcct gtgccggcat gaccaaaaac atagatcgtt tttttattaa
    37801 acttattaag tgctttatcc aatacattta tccagcttct catattggca cctgcattgc
    37861 ggtccacata gggatggcga cggttaaata caagatcgcc gcaatgaaca atattagcat
    37921 gctgaaaatg gataaagctg tcgccattgg tatgacctgc gccaaaatag tgcaggcaga
    37981 ttttctcttt gcccactttc tggcaccagg tattggtgaa ggtttgatca ggatagagtt
    38041 gtttgtcttc agatttattt tgcctggctg aattttcctg gttctttttt gaattttcat
    38101 gcgccagcac atgctccact aatcctttaa aagaaatgtt accaccactg tgatcaccat
    38161 gatggtgtgt attaatcaat aatttgaaag gctgttcact tttctttttc aactcatcta
    38221 tcaggtgttt gctctggtca ggaaattgtg aatccacgac tacggtacca tccttcgaga
    38281 gcagaaatgc aatggtgccg cctctttctg taaaaattcc cacatcattt cgcagcatgg
    38341 ttattttcca gggttcctgg aacagggcag aaaggatatt gtgttgtgct aaagtgaatg
    38401 cacccattgt gaatgcagtg ttttgaagaa aggagcggcg attcatgaga ttaatttttc
    38461 agataacaag ttaattcgat tttagttttg aagcggtaat tctttaaact catcccggtc
    38521 accgaatgtg gctaagttaa tactcaaatt ttcttaatcc cggtgcttta aaccatgtta
    38581 tgattaccac taacaaagtc atacaccctc caaagatgac ggatggtacc atacccattg
    38641 cggtagcagc cacaccgctt tcgaactggc cgagttcgtt tgaggaattg atgaagatag
    38701 aattgaccga ggaaacccgt ccccgcattt catcaggtac aaataactgt acaacagttc
    38761 cgcggatgat tacactgaca ccatcaaaca agcctccggc gaataaagca aaaaagctca
    38821 accagaaatt ttgggatata gcaaagacca ggatgcacag tccaaaaccg gcgacaacat
    38881 ataacaatgt ttttccctgt tttcctttca ggggatgaga agtaagccag gcgatggcaa
    38941 caaagtttcc caggtaagtg gcggcgacca accatcctaa tccctgcgga ccaacatgca
    39001 aaatatcatt ggcaaaagca ggcaacaggg caacagcgcc cccaaataaa acggcaaaca
    39061 tatcaagact catcgcacca agtaatgctt tttgtttaaa aacaaaacgt aatccgtctt
    39121 ttacaccatc ccatgtttta ctgtttccct gccagctaac ctgttttggt tttattcttt
    39181 gaaatagaaa aaatgcaata cctatcagta cgcatacggg tgtgaatgcg atggtaatat
    39241 tagtgtaccc catcagtaaa ccggcaatag caggtccggc tactgctgca acaagccata
    39301 ccatactatt aatagaaaca gcctttacca atgaattctg cggtaccaat tgtgccagaa
    39361 aagcattgaa agcagaaccg gtgtaagccc ggaccgcacc cgttccaaat accagcccat
    39421 agatactcca ctcaatgatg gaccgtgaat aattatcctg catccattgt gaagtaacaa
    39481 gcagcattcc aagcatcagg aaaaaataaa tggcgatgca gatacttatc agcctgtgtt
    39541 tgttgctttt gtctacctta accccggcgg gcaatgccag cgcaatggca ggtatcactt
    39601 cagaaagtcc aaggaaaccc agtgccagtt tgcttcccgt gatttcatag agtcgccagc
    39661 ctaataatac cggagtcatc cggagaccgg caataaatag aatccgggct gtgatgaacc
    39721 aggtaaattc tttattaatg accgtggaga aaatattttt tatagaaaaa cggtcactca
    39781 tggaagtgta atataatgct gtccgttttc atattctttg tcaagagcat catatattac
    39841 ctggaggtgt ttttcatttc ccaggaaatc agcattactg gcatctacaa aaagtgtttt
    39901 gatattgtgt tgtttgatat aatgcgtata ggtttcctgg atattgaaca aatattcgtc
    39961 ctgtatattc tgttcgtaag ccctgttacg tttttttata ttggactgca acttggcaac
    40021 aggggcatgg aggtaaatga gtatatccgg ctgtaccagt tgctggtgga tgatatcaaa
    40081 gagtcgttgg tacaggcgaa attcatcatc cggcaatgtt actttggcga ataataaaca
    40141 tttggtgaac aggtagtccg aaatggttag tgcctggaac atgtcttttt gctgtaataa
    40201 ttctttttgc tgtttgaaac gttcggccat aaagaacagt tccagcggga acgcatattg
    40261 tccgggattc tcataaaatt tcggaagaaa aggattatcg gcaaactctt cgagtatcaa
    40321 acgggcatta tagtgtttgc tgagcagatg tgccagggtt gttttaccgg cgccaatatt
    40381 tccttcaata gttataaaat ggtagttcat gtgtcaatag tagaaagcca atagctgata
    40441 gaggtcgcaa aatactctaa atgcaaaggc ataaaataat tagctgaaga ttgtggaaaa
    40501 aatcaggaat acttttttac ttccagctcg tccttgcatt cctgtagcag ttcagttatt
    40561 gtttttttga gtacaggatg tataatctcc ggggctatct ctgctaatgg taataatgca
    40621 aatcgcctgt tttgcatttc agggtgaggt aatttcagaa actggtagtt gtgtttttca
    40681 ttattaaaaa gaaggatatc gatatcaatc aggcggggtc catatttctc ttcccttacc
    40741 cggcccattt ttttttctat tttaagaatg cggcggatca attgtcttgc gttcaacgga
    40801 gtttcgatca ttattgcctg gtttaagaag gcaggctgat cagtattgcc ccatgccgct
    40861 gtttcatata aagaagaggc agcaatgata ttgccagttt gtgcctctac taattccctt
    40921 gccatggcaa gattatgtac ccgttcgccc aaattaccgc cggtaagaag atatgctgta
    40981 ttcataatac gggtcaaata tctttatttt cagcattgaa atgtaattaa atacatttgt
    41041 ggcccaacat gaaaaatagt cagcaacctt atagtcagtc cagggcttta tgggctatta
    41101 caagagccag tttcaaagca atattttcca atcccagcgc tatcatattc agtatactgt
    41161 tccccattat ttttgtgttg atattcgggg cttttggcag cggcggggga gtcagttacc
    41221 ggattgcact tacgccggac agtgatacga caaatgattt ttaccaggga ttaaaacaaa
    41281 cgccggttat ccggatagtt agttacagcg attcggcctt actgaataaa gacctgcaga
    41341 agggcaagct tactgctatt cttaccctta ccaggaatga ggatactgca caaaaacctg
    41401 aaataacagt taaagtaaat tcaaccactg cgagtgcaaa tactatcggt tcgttcatcc
    41461 cggtgcttga ttatatcaga ctaagaattg aaactgcaaa tgccggaatt gagcaggaga
    41521 gtattgcggt tgaagaaccg gtgataaaag aagtaagaaa gtaccgccag atagatttca
    41581 ttttgcccgg acaattgggt ttttccatat tatttgctac actattcggt gttgctttta
    41641 ccttttttaa cctgagagag cagttagtat taaaaagatt ttacgcctct ccggttaata
    41701 agataaatat actgttgggt attgggatga gccgcttatt ttttcaactg ctcagtgtgg
    41761 tggtgttaat attgtttggt catttcttta tgaattttac tttacagaat ggcattttta
    41821 cttttttgga gataatagca atgaccatta ttatgctatt cctgctgatg ggggtgggcc
    41881 tgattttcag tagtattgct aaaagcgaca cctctattcc attgctgata aatttatttg
    41941 gctttccaca gatgatgttg tcaggtactt ttttcccgat tgaggttttc cccaaatggc
    42001 tgcaggagat atgcagggtg ctgccactta cccaatataa tgatgcggtt cgaaaaatct
    42061 catttgaagg gttgagtatt tttgaatgct ggaaagagat cggaatactg ggaatctgga
    42121 tagtaataat ttattttatt actgccaggg tgctgaaatg ggagtaggaa ttgtcaaccg
    42181 ggtgggtaag ctgttatttg taaaattcat ttgatttaaa ttatgcgttt attaaagctg
    42241 gccataataa gttttattgt tttttttctg cttattacag ctatctctct atttatacct
    42301 gctcatgtcc gaatttcgaa agcaactgat attaatgcaa cgaaagactc agtaatgctg
    42361 caggtaaaaa atgctgctaa ctggaaaaac tggtatccgg gagcagacac actggaagta
    42421 ttaaaaatag atggagttgt gaaaggcatc ctgacaaaag ggcaacaggg aattataatt
    42481 accgggtcgg atgatagtac tgtaatggca ggaaatgcag gtacagcccc cggtaaagga
    42541 caaatgggtt ggaatatact tcaatcccgg ggcaaagggc cagtcaccgt tcaatggtat
    42601 atggatttcc ggtttagctg gtacccatgg gaaaaatttt ccagcctttt actggaaaaa
    42661 agatatggat tactcatgga acaggggctc gataaactga agaagttcct cgaaaaataa
    42721 atgcaggttc ctgtaatctt gttaacttca atgctcaaaa atcaacaaga tgaaaaatat
    42781 attattagtg ctggctgtat ttgctgtttt caatagctat tcccagcaat caacccggaa
    42841 aagtccgcat gacacggtga gttctgccaa tacaatagtt acctacggca gaccttttaa
    42901 aaataatcgt gaagtttttg gtggattaga gaaatttgga aaagtatgga gagtcggagc
    42961 cgatgaagca acgactatca gttttgctaa agacgtaaat tttgccggtg ctggtattaa
    43021 agccggtacc tatactttgt ttgccatccc tgaagaaaag gagtggacca ttattcttaa
    43081 ttcgcaatta gggcaatggg gtgcttatgg gtacgaaaaa aataaggata agaatgtggc
    43141 ttcagtaaaa gttccggtga tgaaactgag caacgtggta gagcaactta cgatccgttt
    43201 tgcagaaaat ggttcactgg ttatcgaatg ggatatgacg caagtaaccg tgcctttaag
    43261 tttctgattc taaatgacta tttataaagg ctgtccatcg ggcagccttt ttgttttgcc
    43321 tttcgtctct tagtaacttt aagtaataaa tctgccaatt atgacctgtt taaaaagagt
    43381 cctggtaagt gcactttctc tgtcgcttgt tttaacaatt caagcccagg taacagaggg
    43441 tgatacactg ctgtttcaaa gagacagtgt aattgaactg gagccggatc ctaccactat
    43501 tttttttcag cagggtgaaa aaacgaagat ttctgggaaa gtgatgctgg ttactaaaag
    43561 caggaaagcc atttccttgg ccaatttttt aaaaagcatg gtggcagatg taacagattt
    43621 tccgttgtat actgatcatg cttttgcaga tatggataat gatggtaaga aggaactgtt
    43681 ggtttccaat tatacaggag gtgcccattg ttgcgatgag atctacattt ttaaaaatat
    43741 tggcccgaat aaataccagc atgttgctaa actctttgcc ggtaatacca ttattacaaa
    43801 ggaaaaggaa tttatttatg attttcatga acagtttgga tatttcttca cttgttttgc
    43861 ctgcggttat actgatacta cggatggtgc cccggtagaa attcgcagca ttacacttaa
    43921 gtataacaag gggaaaatgg tggttactcc cggcgaccag gaattgaaaa gtatcatcaa
    43981 tgataacctg ggaaaactgg gtgaacagcc ttatgaaaag ttgagcgatg atatagacca
    44041 ggataacggc ctccgtaaag agtttgcttt aaacctggtt gtgcattatt attctttcgg
    44101 gcgaaatgtg atggaaactc aaaagctttt caataagtac tataaatatc ctgatgccaa
    44161 aaaagtgtgg gcagcttttg taaaacaaat gcagtacatg aaaggggata atgatttctg
    44221 atcaggcacc cgaaatgaat ttgagatttc gctgaatgcc cttccatttt gttcgcttca
    44281 acggggaatg tttgaatata gttttaaaac tttcttctgt catcagctcc cattcttttg
    44341 tactcaggtt tagtatttca gggatgggtg caaaagaagt ctcatcatat ggcttgctga
    44401 agcggttcca gggacaaaca tcctggcagg tatcacaacc aaacatccag ttgtcaaacc
    44461 ggcctttcat agcatcggga atgatcattt ctttcaactc aatggtgaaa tacgaaatac
    44521 acttactgcc atcaattact ttatcgggca atatcgcttc ggttggacaa gcatcaatac
    44581 atttggtaca ggtgccgcag taatctttaa caaaaggatc atcgtattcc aattcaaggt
    44641 cagttatcaa tgttgcaata aaatagaagg agccgctttg tttggtaatc aggtttccat
    44701 tcttgcctat ccatcccaac ccgcttttct gcgcccatgt tctttccagc accggggcag
    44761 aatcaacaaa acctcttcct tgtatttccc cgatatgtcc ctgcatttct gcgattaatt
    44821 ctttcagttt ctcccgtatc acctcatggt agtcaaggcc ccgggcatac ttcgaaatct
    44881 taggggcttc cggttgctgc tcctgtgcag gataataatt taataataaa gtaatgacgg
    44941 tccgggcacc gggtaccagt ttggaaggat cgatacgcag atcaaaataa ttttccatgt
    45001 actgcatacg gccatgcatg cctttctgta accatttttc caaacgtaca gcatcttctt
    45061 ccagtttatg agcccttgtg ataccgcaat agctaaaccc cagccggcca gctgtttttt
    45121 tgataaaatg ggtatgtgta tgcaacaggt tcagggggag ttaatattta ttttattcta
    45181 ttctattgat tttattgcca ctttaatcat tgactagttt ggtttttttg cgtaaaattg
    45241 acctttgttg aacgaattta accacattta accatcacta tgcgttcaaa actgtacttc
    45301 ctgtgtattg ggttattatt tttttccggt aatctttttg cgcaggaaaa atctaacgta
    45361 aaatttggca atgtttccga aaaggatttt aatacaaaaa tttatcctat cgacagcaat
    45421 gccagtgctg tggtgatcgc agatattggt tatagcgcaa ttgaggggaa tacgaagggt
    45481 tggttctccc ttgtttacaa acattttaag cggttgcaca tcctaaataa aaatggctat
    45541 gatgcagcta atgtttcaat ttccttatat gctgatggtg atgcggaaga agagttgaat
    45601 aaattaaaag cagtgaccta taacctggag aatgggaaag tagtagagac gaaactggat
    45661 gtaaaggcat ctgtctttaa agataagatc agtaaaaaca gggtggttaa aaaattcacc
    45721 ttcccgaatg tgaaagaagg ttccatcatc gagtatgaat ataccattac ctcagatttt
    45781 ttgcagaatc ttcagccatg ggaatttcag ggagcctatc cccgtttatg gagtgaatac
    45841 aatctggctt taccagaatt tttaggctat gtttttctga cccgtgggta taaaaaatat
    45901 gatatcaatg ataaaaaact gcgttcagaa agttttacca ttttagatag tcgtggtatt
    45961 ggcgcttctg aaaggtacca gtttactgcc aatgtatcag acaacaggtg ggtaatgaaa
    46021 aatgtgccgg cgttaaaaga agaaagtttt acttccacca tcgataacca tattgccaag
    46081 attgaatttc aattgtcaga ataccggcag ccactcactt accggaatat tatgggttca
    46141 tggcccaaat tggccaatga cctgatggag gctgattttt tcgggaagcc tgttaccaaa
    46201 gataatggct ggctgaaaga tatagttaag ccgcttattg aaggagtgaa tggcaataaa
    46261 gaaaaggcac ataagatatt cgcctatgtt cgggacaatt ttacctgtac caattacagt
    46321 gatctttttg cagaacaaac gcttaaaaac ctggagaagt ctaaaagtgg tacagttgct
    46381 gagattaacc tgttgctaac cgctatgctc aggtacgcag atattgaagc tgacccggtc
    46441 attctcagta cccgttccaa cggaatgact tatcccctgt acccgcttat tacgcaatac
    46501 aattatgtta ttgcaagaat aataattgat ggtaaaacat tttacctgga tgccagtgaa
    46561 ccccgcctgg gatttgggca tttgcccgtg cgttgttaca acggacacgg aagagtgatc
    46621 aacaagacag ctgatgcaat cgaattggaa agtgattcat taatggagag aaaaagtaca
    46681 actgtatttg tggtcaacga tgagaaagga aatatcgtgg gtagtatgct gcaaaacccg
    46741 ggttattatg agtcttttaa cctgcgggag cgtatcaaag aaaaaggaaa agagcaattg
    46801 ataactgata taaaaaaggc ttttggtacg gagatattgg ttagtaagtt cgaactggat
    46861 tcgcttgata agtatgagga agtgctggga ataaaatatg attttgattt taccggtgaa
    46921 aaagaagata tcatttacct gaacccgatg tttggagagg ggtataaaga aaatccattt
    46981 aaatcagcag aacgtcttta tcctgtagaa atgccctacg gttttgatga aacatatacc
    47041 ctgcaaatgg atgtaccttc cggctatgaa gtggatgaat tgccaaaatc aatggtggtg
    47101 aagttgaatg aggaaaatga aggaatgttt gaatatcgta tttcccagtc tggcagcggc
    47161 atctcctttc gttcccgcat tcgtataagc agggcctact ttatgcctga agaatatgag
    47221 atgttgcggg aattctttaa cctggtggta aaaaagcagg ctgaacagat cgtacttaaa
    47281 aagaaaaaat aatttatgcg caatagattt atcctgttga tttgcctggc aacactgatc
    47341 tccttagccg ggatggcaca gagttcctat gccgttgtgg ctataccgga agcactgctc
    47401 aaaaatgcaa gtgtaattaa aagaacagaa gatatccggt atgagatcac cgagggtaat
    47461 aaagccagct tttaccagaa agttgcttat acgatcttgg atgagcaggg tgaccgctgg
    47521 gccacacatg cagaatggta tgataaactc cgcagcgtgg aatcctttga aggttccttg
    47581 tatgatgctt ctggcaaaaa gatccgtagc atcaaaaagg gagaaataaa agatgtgagc
    47641 ggtaatgatg atgcgagcct ggcagatgat aaccgggtga aatggcatag ttttttttat
    47701 aaggtatacc ccttcaccgt tgaatacgaa acggaaattc gttttaaagg gaccatgttc
    47761 tctcccgact ggattcccca gcaaaaaggg gcgatgagtg tacagcacag ccggctcacc
    47821 gtgctcagcc cggtcagcaa cccgcttcgc tataaaatgt ttaactataa aggggaaccc
    47881 gttgttgccg aagtgaagca gggaaaatct tacacatggg aagtgaaaga tttgccggcg
    47941 atcaaagaag agtttgctac accgtcctgg catgagatca ccaccagcgt ttttcttgcc
    48001 acagaaaaat ttgtactgga agattaccag ggcagcaatg caagctggaa agattttgga
    48061 aaatttgtgt atgacctcaa aaaagagagg gatgaattac ctgaggaggt gaaacaaaaa
    48121 gtgcgtcagt tgacaagtgg ggtcactgag gataaagaaa aggtaaggcg gttgtatgaa
    48181 tacatgcagc aaaatactcg gtatatcagt atacaattgg gtgtgggtgg ctggcaaccc
    48241 tttgatgcca aatatgtggc atcaaaaaga tatggcgatt gcaaagccct cagtaattac
    48301 atgtttgcct tattaaaaga agcaggtatc cgctcggtat acacggtgat cggttccggc
    48361 aatgataatg attacctgat ggttgacctg ccttcatcgc agttcaatca tgccatatta
    48421 tttgtgccgc agggaaaaga tactgtttgg ctcgaatgca ccagccagac aaatggtgcc
    48481 ggttatttgg gaggcaatac cggcaaccgc tatgccctgg ctgtagatga aaatgggggt
    48541 gcattggtcc ggacacccca ctatgggatg aatgaaaatt tacagatccg gagtattaaa
    48601 gccaccttgg ctgaggatgg caccctgctg gtaaaaagca atacgcatta cagcggtatg
    48661 cagcaggata atattcatgg tctcattaat aatctttcaa aagacaaagt gaaggaatac
    48721 ctgcatgaag agctggattt tgccacttac gatgtgaacc agtttaatta tagccaggat
    48781 aaatcaattc ttcctgtgat agatgaaaca ttggaaataa cggttagcaa ttatgctacg
    48841 gtcaccggca agcggttgtt cattgtccca aacattatga acaaaacata ccggaagcta
    48901 agtgcaaccg atgaaagaaa atatgatatc gatctggggt ttgaatacaa ggatgtcgat
    48961 tctgtggaga tcgaactgcc caaagggtat gaacctgagg ccatgccgca gccggtctct
    49021 atcagcagcc aattcggaaa gtacagtaac accgtgaaat tggcaggtaa taaattgatc
    49081 tactaccgca gtatcgaaca atacagcgga cgatttcctg ctaaaacgta tcctgacttg
    49141 gtgaagtatt atgatgctgt ttacaaagct gaccgcagcc gggtggtgct ggtgcgaaat
    49201 gaacagccgc tgaaagggtt ctgataatta taacctaatt attcttttta ctgaaatagt
    49261 atttaatgca aaagcttcta tctgcctttt ttattctttg ctcagtcagc tgtttttccc
    49321 agcaaaaccc tacggtcaaa ttcgggaaga tcagtattgc agaaatagaa agaaagaact
    49381 atgatgttga taccagcgct gaagctgtcg tgattgccga tataggcgct acgcagatca
    49441 agggaaaccg caatgaatgg ttctcgctgg aatttagacg ccacaaacgg atacatatcc
    49501 tgaaaagaaa tgcttattcg catgccaccg ttgagatttc tttatataca gatggtaaaa
    49561 tggaagaaca attggatgac gtgaaagctg taacttataa ttatgagaac ggtaaaatag
    49621 ttgaaacaaa acttgatgtt aggacaggag ttttcaagga taaactggat aagaacaggg
    49681 tggtcaaaaa gttcaccttg cctaatgtaa aggagggctc tatcattgag tacgagtata
    49741 aagtaacgtc cgatttcctt ttcaatttac aaccctggta ttttcaggga gacattcccc
    49801 gtttatggag tgaatactcc gtcg
//
LOCUS       DAORTI010000012        49167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_403_length_49167_cov_6.227236, whole genome shotgun sequence.
ACCESSION   DAORTI010000012 DAORTI010000000
VERSION     DAORTI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 49167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_49167_cov_6.227236"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..987
                     /locus_tag="PLO70_03160"
     CDS             235..987
                     /locus_tag="PLO70_03160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73482.1"
                     /translation="MTASNGKGHGIHSPFIFQFITRVLNDHTQYKEYDKVEKLRQQFL
                     NDRTILTIQDFGAGSSVSKTNQRSVASIAKNASKPKKLAQLLFRMVRLYQPSSILELG
                     TSLGITTAYLSLAKPDAKLFTLEGAGEVAALAEKNFLVSGFQDISVVKGNFDDTLSVV
                     LEGLPTVCFAFIDGNHRKEPTEKYFSQLLAKINNESVLVFDDIHWSREMEQAWETIRN
                     HPSVRCTIDLFFIGIVFFRQEFHEKQHFVIRY"
     gene            1111..2226
                     /locus_tag="PLO70_03165"
     CDS             1111..2226
                     /locus_tag="PLO70_03165"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HQZ73483.1"
                     /translation="MTIGVLKEPAHETRVSLLSELVPSLLKKGVTVLVESGAGEKAFC
                     SNAAYENAGAQIKSRSEVLAAADIVLSIFQPDPSEIDRLKSKVIIGVFQPLFNVEEMK
                     SWAVKGLTTFSLDMLPRTTRAQAMDVLSSQANIAGYKAVLTAANLYSRYFPMFMTAAG
                     SIKPAKVLILGAGVAGLQAIATAKRLGAVVEVFDTRPAVKEEVMSLGAKFVEVEGAAD
                     SSKAGGYAVEQTEEYKQKQQQKIADSIAKADIVITTAQIPGKKAPILITEEMIKAMRN
                     GSVIIDIAAATGGNTPFTKNNETVNFNGVSILGNSYLPSTMPSDASKVYGKNVSIFLQ
                     LIIDKEGKINLNWEDDLVKGCCITHEGEVIHERVKGN"
     gene            2249..2560
                     /locus_tag="PLO70_03170"
     CDS             2249..2560
                     /locus_tag="PLO70_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQZ73484.1"
                     /translation="MESILKWIYANEQMIYILVLVIFLGFIVISRVPAVLHTPLMSHA
                     NAISGVVIMGAIIVMGRADADNYAALALGLLAVILGTINVVGGFVVTDRMLEMFKKKK
                     K"
     gene            2610..4022
                     /locus_tag="PLO70_03175"
     CDS             2610..4022
                     /locus_tag="PLO70_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQZ73485.1"
                     /translation="MGVSVLSIIYIIGAVTFIAGLKMMSDPASARKGNIIAGLGMLAA
                     IIGTIFLYQNEDGSLLKNHIWIFGGILIGSVIGVLAAKKVKMTAMPEMVSLFNGLGGA
                     SATLISLVEFNHLSDLNFPAESATPGIMAILFAGIILGNITFSGSMIAWGKLSGRVKD
                     FAFKGQHILNILLLVISIALSVYITSNLQGANTTILFYTVFLLSLVYGIFFVFPIGGA
                     DMPVVISLLNSLSGLSAACGGFLYDNKVVFTGGILVGAAGTILTVLMCKAMNRSLTNV
                     LIGSFGGSKAAGDKKEQGSYKEISLNDSAVVMSYASKVMIVPGYGLAVAQAQHTCHEL
                     QKMLEAKGVEIKYAIHPVAGRMPGHMNVLLAEADVSYDKLIEMEQANEEFPAVDVVLI
                     LGANDVVNPAAKSDPSSPIYGMPILDVGLAKNVIVVKRSMKPGYAGIENDLFFQPKTS
                     MLFGDAKKVLQDLVGEIKSL"
     gene            4065..5417
                     /locus_tag="PLO70_03180"
     CDS             4065..5417
                     /locus_tag="PLO70_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73486.1"
                     /translation="MQLPAILLDALQNARGFDKNAFERIHASGEQVTSVRLNPLKPPK
                     GITDHPIHQLTRIPWTAYGFYLEERPSFTFDPLFHAGTYYVQEASSMFLEQALKQTVD
                     LSKPLKVLDLCAAPGGKSTHIQSMISKESLLVSNEVIRSRANVLKDNIIKWGCENVVV
                     TNNDPRDFAKTEGYFDVIVVDAPCSGSGLFRRDPDAIAEWSENNVQLCSQRQQRILAD
                     VWPALKKNGMLIYSTCSYSKEEDEDIIQWLLQELPVALCPLSFEPSGGIVASGGGYRF
                     WPDKVMGEGFFIACFQKKEGEEELSFKIRNRPEQLNKKEMEIVEKWIKNEGKVFIKHL
                     NTVYAWPELLENEFSFLLEKLRVVYSGILVGELVRDKLVPEHALAMSNSVADSIENIE
                     LTKQEAIKYLQRKDLQLHTATKGWQLASYSGHPLGWLNVLANRVNNYYPKELRILKDI
                     "
     gene            5469..6449
                     /locus_tag="PLO70_03185"
     CDS             5469..6449
                     /locus_tag="PLO70_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQZ73487.1"
                     /translation="MKKFVLSLLTLFISVSFIVAQKGDLLVKNGDKGLFLEHKVVAKE
                     SFFSVGRLYNVHPKFIASFNNLDMGKGLQIDQKLRIPLTDTNFTQKGNSGTPVYYKAD
                     VNEGLMKISKANNDVKLANLRAWNNLTGDNAGDKKKLIVGFLLSKEMPTITIGTSPKT
                     KEPTVKKEEKPVVAEPTVEDKPVVKNEEPVVKKEEKPVVTEPKVDDKPVVKTEEKTIP
                     GGQGYFRSHFEQQIRKSPITKDETVTAGIFKTTSGWQDEKYYILIDAVAPGTIIKIVN
                     PTNSKAVYAKVLGEMSGIRQNQGYNIRISNAAATALQVTEQDKFIVKINY"
     gene            complement(6443..7501)
                     /gene="fmt"
                     /locus_tag="PLO70_03190"
     CDS             complement(6443..7501)
                     /gene="fmt"
                     /locus_tag="PLO70_03190"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQZ73488.1"
                     /translation="MIEQQIKQKDIGPNTFNLSLVSLRIVFMGTPEFAVASLDALVKA
                     GYNIVGVITAPDKPAGRGMLLQQSAVKKYAVEHNLKVLQPEKLKNKEFIAELKSLNAN
                     LQIVVAFRMLPEIVWNMPSMGTVNLHGSLLPQYRGAAPINWAVINGERETGVTTFKLK
                     HEIDTGDILLQESFAIGEDETAGEVHDKMKDIGAQLLVKTVKGLADGSLQEKSQDLII
                     HDLQPAIDNTRVTHRQKQMPVDSSFLKHAPKINTSTCRVDWSLPAERIYNLIRGLSPY
                     PAAFTILNEKMLKIFKAKKEIIFPKVTEGDYETDGKTFLKFACEDGYIHLLELQMEGK
                     KKMEITEFLRGYRFNSNQ"
     gene            complement(7601..9271)
                     /locus_tag="PLO70_03195"
     CDS             complement(7601..9271)
                     /locus_tag="PLO70_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HQZ73489.1"
                     /translation="MRKIIFCFCLAFFSNTLFAQPHNFLLEGRWTGTYGNNEKDNPYY
                     FSFEFLPGGKMNVVNQNNKILAEGTYSVKEDNVRIVYKYVNDVQQYACGGDIKKDSNT
                     ISGYWQRLEDAGSNSKFTQRGRWTMKKQELNNNPATKNDSLLVFNPVRKKDIVPVKKK
                     DFNFSYPTTCTDFPPVGSRPLPARIPTNYIAQYKINTDGTVSPFAFIRQSLATYTEKM
                     WEPGETITVSFDIVGGSIFLIDLVKQYAKEWELYANIIIEFVTNLADGKIRVGFKEGA
                     GSYSMIGRDALLAPVTQTTMNFGWLTSLPAGSPFIRQVILHEFGHALGFVHEHQTFGT
                     AIPWDKEKVYAHYAAAPNYWTREQVNQNIFTKFSYTSTNYSSFDSKSIMLYPVPKEFT
                     TTGDSIPWNMDFSPTDKQYAGLFYPFPTPPPAASGILRTGDDCDEIAFVVEYDMVDRD
                     KVEFVFQLGEINGKKVSWWKEIGIPMINSNQEAKLWIQNHSLIPSENRTLASGQLPFT
                     EINKDAGISFAKAKVLGAHTPLSYKWNVLQALMGGCRVTLTWRKDSCL"
     gene            complement(9308..11158)
                     /locus_tag="PLO70_03200"
     CDS             complement(9308..11158)
                     /locus_tag="PLO70_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73490.1"
                     /translation="MKYILIVLFCMITSTLIAQEKADCNTLDVNKVPGKWVWQNIAPS
                     IQDPIPASQWKFSDPIRKEMQRIMPLSLDGLHATNSIAFPKGKAFWYSTTSPAAYECY
                     LMLKKFECLKGYNELKPEAVTGCWAYFSMNQIEGEKFPLPEQGTGITFNQSKIRVTNI
                     EVQTDATGNKIIYSQYRPDEIIKHCYFFSGRKDLPWRKMSNSELFTAYKTHHEKRVKQ
                     EVLRFEKLVADDQKKYNGLSAAEKQNQNYWPEIIRKNKEILQKHKDELEKISNWYNVA
                     MQQSTINAVAYVTSVNESHFYPERLMATPENGYNAWVDNLDFFDKTKPKDEPQCIAFF
                     VRRDDDDLPKKNFMDLFHSQFNMDVLAKMVGEPAKKPNGINNLNASVTEIKKETNSKQ
                     EDKGPVNASFDNTADGQFPMGWLGMKNAIVQTNKGSKWLTITKDGYWYPRQYNKAIKD
                     KFNLSFDLQWNEDIAYNSGSFTVTLGDIDYDNTGERYRLDDNQEMFWSLYNGYAGNFN
                     RVILWFDPYWNGGGTLTVYSYDKRESLAFNKRIVLPDFYKDKNNHKLQLQRKGNGLVV
                     IDNGKTIADLRDVFLTTAHYNIYTFSRYKGEFSDNKNDVFYLGNIKTTYE"
     gene            complement(11170..12513)
                     /locus_tag="PLO70_03205"
     CDS             complement(11170..12513)
                     /locus_tag="PLO70_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73491.1"
                     /translation="MKHFSILLLFFFSILLQSAGQSIKKYDIFSYSAPAGFVLKEQKE
                     RLLYEKREGNSFCQIHIWAAQQGSSDPAANFKTDWEHFAVKPYNLTEPPTTQTEKQNG
                     WEVVTGASQAAMDGIPFIVAVATFTQNDISWCAVSIFNDEKYAAVIDKFILGIKADSR
                     KMVRKPNQATQQNSIPVSNNNTGGITNSTTSFDDGWTATVNNDYVKLTKAGTELRLHY
                     TDKALDDARPNTIDAPEYYWSKYVEPYFNVSNVQKWSGVQYPVIYHIQANAVKKKTGK
                     SCFVAIKIVYSGGARPIVVIAPDQNNYQQQFPHPNDIDPMLNANRFALTANDIIGTWK
                     GSGGGGVEYYNVYSGTYAGMSAVSSTDEFTFNSNGTYSSTYRSASMNSGGAQFGGQDY
                     KGNFSVTDWSLTVTNRYKAKTTTYKAQLIAVKGGFLLYMEDSDNSSMKYTLFKTK"
     gene            complement(12543..14072)
                     /locus_tag="PLO70_03210"
     CDS             complement(12543..14072)
                     /locus_tag="PLO70_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73492.1"
                     /translation="MKKKVFLSCIYSAMIFIAAAQNVGIGTTTPLARFHVTDSAVLLS
                     GTVSIPPFTTTINPPVQGAGTRTMWFPALGAFRTGYVMDTEWDRDNIGILSFATGHST
                     KASGRFSTSMGQYTSAINSVATSMGFGTYASGEVSTSMGNGTEASGFSATSMGASTIA
                     SGNYSTAMGQSNTASGLVSTATGRSTTASGNYSFSTGEQTFAKARGSFTTGIWNDNTD
                     NPDVNLTAPTDRIFQIGNGSGSIRSNALTVLRNGNVGIGTTNPGYPLHVQKSAANIFA
                     ASIENQDATGWGLEVRTADAGSTRNALEVYTGGTSRFLVRNDGNVGIGNIDPGYRLDV
                     SGRMRIRSGGDLNNSAGIFLNNTLNTDIPAFIGMQSNEQVGFYGNSSGWSFVMNTSNG
                     NVGMGTTSPTTKLEVNGFTKLGSDAPSIKVKKLTGTTASTEGGDTNIPHGLVFSKILS
                     VSVLVESETNIYTPPSSTHITGVQYNYDIYNNSIWVFNTPGNSANILSKPFKVLITYE
                     E"
     gene            complement(14075..15730)
                     /locus_tag="PLO70_03215"
     CDS             complement(14075..15730)
                     /locus_tag="PLO70_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ73493.1"
                     /translation="MKNTFYLLFILVTATIQTNAQCWVKVSAGYAHTLAIKTDGTLWG
                     WGDNTSGQLGNGTNNNSNAPGQIGTATNWQVIAAGAEHSMAIKTDGTLWAWGRNSEAQ
                     LGDGTINNSTVPIQIGTATDWATISAGTEHNIAIKTDGSLWAWGGNQYGQCGNALPPN
                     SVATPLQVGTATNWQNITTGPYNSIATKSDGSLWSWGYNLNGQLGDGTYTNKDVPTAI
                     GFDNDWGQIAAGWFHSIAIKTNGSLWAWGDNNYGALGDGTNTNQNQPTLIGAATNWQS
                     VSAGEEHSIATKTDGTLWAWGRNFRGQLGDGTNANRNSPVQIGTATNWGQIAPGADYT
                     FSIKTDNSLWAWGNNDAGQLGDGTNAERNIPVQISCNFILPVTWLYINGQWQSNAAQI
                     KWATTSETNTKYFEIEHSSNGINYSKIGTVGAAGNSSLTQRYDFLHTSPTLGKNYYRI
                     KQIDLDGRFTYSTIIVLHKSDLRTNTFIAPNPVHNETTVYFGEEGSKTLQLINLNGNI
                     LRTDKIGPSINRYTIKLDKLSPGLYLLRLQTTNGTTTYKIIKQ"
     gene            15899..17839
                     /locus_tag="PLO70_03220"
     CDS             15899..17839
                     /locus_tag="PLO70_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ73494.1"
                     /translation="MKRFTLLFLLMCDQNVYAQTDATRDSMLNVLLVAKEDTNKVHAL
                     ITTGQYVEYNNLEEAKSYYLQAYELSKKLNYTMGVLKYYSNYTAVLNIQARFDSALVL
                     TKYALQLANKFGNEERIIIAQQNLSATYAYLQDYESALQYLLPSVAYFEKTKNDARLS
                     LIYDNLGVIYRETKQYNKALEYHQKALAIARKAANSYDIANVLSNLGNSYSSLKEYDT
                     ALAVMQESLLIAKKENYDNIVSNVTSNIGSLLIKQGRYDSLNFYCAEGLRLAEKLGDS
                     LTMVNNIYGLALAAFYDKDFKRSEEEAVAGFAMAKQRNYAEPLQLFADILSELYLVKG
                     DLKNYDYYNRLSIELAEKYFSERMQRNVQTLDKKFETEKKEQQLQLQQVTIRQKSTLN
                     YILIGSTAALLLISLLVYRTLKQKRKLQEQKITQLEKEKLLLATQSIVKGQEEERNRL
                     AQDLHDGLGGLLSGVKLQLGAMKGNLILSEEHGRTFNNALGKLDESISEMRRVAHNMM
                     PEALLKLGLQQALQDYCDGLAESQPFTINTEFYGLEKRLESSVEIVVYRIVQELLNNV
                     VKHSGATSILAQVMRQGKNLTITVEDNGKGFGDNVYTNEEGAGLKNIRSRVDYLKGQL
                     DIKSTLGKGTSVHIDCLIEDNG"
     gene            17832..18455
                     /locus_tag="PLO70_03225"
     CDS             17832..18455
                     /locus_tag="PLO70_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73495.1"
                     /translation="MDKIKVLVVDDHPMVLEGMRSMLAQISFVEISGTVSNAFDAIEM
                     IKINLPDIVITDINMPEVSGIDLTVKIRKEFPAVRVVAMSTFKERSYISQMIQSGASG
                     YLVKSASKEEIEEAILSVNEGKLYMSLDISLTSSDKQELNNMPVLSSREKEVLQLIAD
                     GMTNPQIAAKLFLSLHTVDSHRKNLLTKFTVNNTASLIKLAAKYNLV"
     gene            complement(18505..18906)
                     /locus_tag="PLO70_03230"
     CDS             complement(18505..18906)
                     /locus_tag="PLO70_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQZ73496.1"
                     /translation="MKTSLYIKQVKGKGRGVFCKERICKDQVFETAAVLVLPARDYET
                     VTSSHLADYFFNFDKEAGTLALVLGFGSLYNHAVYSNAAYSLNMETRTMSYYALEDIP
                     PGTEICINYAGEQGREFKEWFETRNIDFKES"
     gene            complement(18903..19325)
                     /locus_tag="PLO70_03235"
     CDS             complement(18903..19325)
                     /locus_tag="PLO70_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HQZ73497.1"
                     /translation="MLSLFKPKEILQPTGIEIRQSPIDDRGVFATRTFKPGAVIETAP
                     VILLDRQERELLQTTILFNYYFLLADETTPVALGLGYSSLYNHSYDANADYTISVNKK
                     CLIVKANKMIHPGEEITINYNGKADDHSPVYFQPGITE"
     gene            complement(19325..19588)
                     /locus_tag="PLO70_03240"
     CDS             complement(19325..19588)
                     /locus_tag="PLO70_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73498.1"
                     /translation="MKKTLALFSSLLILAGLKAQTTPAPAVKKETVKPVITIPVVTDT
                     VNTVKPATTIKQTKKAIKFDHIKKGTTVQMKENVQTTTKPHKE"
     gene            complement(19608..20123)
                     /locus_tag="PLO70_03245"
     CDS             complement(19608..20123)
                     /locus_tag="PLO70_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HQZ73499.1"
                     /translation="MRTIILAILFFPFVALAQKGQTVYLKLTDASGQQIKGDALAKGY
                     ERSIDVLSFASAGKNNSQLSFSMNITGASADLKKAMGNGALLPSGTLSVLQPGGTGAP
                     IIMYTIKMENIRVSNCAESMGCNGVITTTSVITAGRIGWTYYQTDATGRQTVSRKYGF
                     DSDSGKEWTNF"
     gene            complement(20147..21535)
                     /locus_tag="PLO70_03250"
     CDS             complement(20147..21535)
                     /locus_tag="PLO70_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73500.1"
                     /translation="MKKIYSFCWLIVSPIIIVAQQQTFDITTFTPPKGWKKQATETAV
                     QLTKEDAVKGTYCIITVLKSIPGSADSKENFDAAWETVVKEMVTVSTAPEMQPSSTED
                     GWEAQSGYAAFENEDSKGVVVLVTSTGYQKMVNILILTNTDAYETEMTSFLESVSFKK
                     TKTPADKPSKTVVQPSAPNTTTKKDGFAFNTTNFDDGWTSTVQEEWVEVKKGDIKVLL
                     HYPKEGTIFPADPEPLTNGAWNILVAPRYKDLKNYKTTYISTYNRPYLGMGNATEIAT
                     GKEVYVVFFRQGETGWLEFITPDKNSFIQQYKFDPETIQWDSETDLLIPLANMVGRNK
                     FAVAASDLKGTWTSDFTGIQQMYHVYTGNYAGMNIHQSNEEFIFNAGNSYHWKLLAVN
                     GMVGNMKFDQVKSSGTFSVLNNWQIKFSKIEDRARTYHAFWSCIKGARILNLLDADYP
                     GSGIYTKYGLAK"
     gene            complement(21564..22913)
                     /locus_tag="PLO70_03255"
     CDS             complement(21564..22913)
                     /locus_tag="PLO70_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73501.1"
                     /translation="MKYCLSFLLIVVNFCSYSQQQSFDITHFTAPKGWKKQTTGSVLQ
                     LSKEDATTGGYCIITISKALPADADSKANFDAAWESVVKKMVTVSAAPEMQSPSEENG
                     WEAQSGYAAFENEGSKGVALLITATGYEKMVTMLVLTNSDAYESEMSAFLESVSLKKP
                     TTTATKQNTSAKKPVQPVSKNDGYAFNTTNFDDGWTSTVQEDWVEVVKENTKILIHYP
                     NKITDAYNPNLMDGLKNAWNVLVAPRYSSAGNFEFKPISGWQSIEFAEADMVEKLTAK
                     SVHVVLFKMNYSNGSGKYIEFITPDKNSFEQQFGAYHPESYGWEKMERMANYNKFAVA
                     ATDLQGKWTSDFFGLTQYVNAYTGASAGANSHASNESFEFAAGNTYKWNLGVASGFVG
                     NIKFQSVKSNGKFSMSGNWQINLSDIEGKPKTSAVHFTCIKNARILWIGETGFGRAE"
     gene            complement(22951..23484)
                     /locus_tag="PLO70_03260"
     CDS             complement(22951..23484)
                     /locus_tag="PLO70_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQZ73502.1"
                     /translation="MKKMLILTFMLHAAIFSVAQNADSLFQRRNKAIKQPVSQTMKPK
                     NIIKNQPLQELLPDLVITRASISKTSTGYIVNYTITNTGTAPVKVNINGIRLFGRVKD
                     NGLEMSSSYIKLLQEGALLNPGGIIDNQFTISSSVLNTLANGIQYKYYLTVDHINDIP
                     ELNETNNTFEINFRYGY"
     gene            complement(23655..25739)
                     /locus_tag="PLO70_03265"
     CDS             complement(23655..25739)
                     /locus_tag="PLO70_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73503.1"
                     /translation="MKNILFLLAVIVCASQTEVNSQSIKGKYRFMADSDGKEASAKAI
                     ITLQFLDNTFKLKAEQPGNIVTDEGTYQVSGSKITITFKTMEQGKKSGNFTFQNGTLT
                     LPFKMLNYAAGSSTWLSTAIVSPNTSTKKQVIDNALAGSVIKSKLYKELDSRAGKSAG
                     TLKGGLAEAYYVQATLYYFRNYKWESLYGFAKASQLQETNGLYLNNFSNLLLELGRIY
                     DAKVLTEELTKSFPNLASPWANQAYIYLKLGNVKEADEAIQMAMRLAPNNGLYCYTAA
                     KIAEEKGNKKEAENLSAKAWSLGYAGNGREAQANNSNANNASPAKTNNANPTSKNAAK
                     PNVAKKPPTGYKLSDWQGNYQAKNISARSGENATDANTKFGAGMATTIINLQTLACVK
                     NFSMQISASGNITGSGEIMYVYQGNAANAVAGMMPSAYSYNGFSTYIKDGFQIRSWNF
                     TGTVTDDGRVEIQGLPSEKLDLRNVNEWQKITPWSPLPPDAAGPAMKGPFHLQLSMSD
                     KKEPFIYIDQSLALNDKLIKKVHYTGLIVKTNESITPTCSTFEPPAPEKCPATESIKT
                     KIAISSKDYIFIDNSNTYTKGADGKVNKQSDTKLASPKEVSTGVSVGVEAGMLSGGAE
                     FNTDGSYEFSVGVGMDTGLFGKDSPVKINQKFELIYDSKCGWGARGTVGAGAGGIAEA
                     SVQGVIFFNKGL"
     gene            complement(25765..27630)
                     /locus_tag="PLO70_03270"
     CDS             complement(25765..27630)
                     /locus_tag="PLO70_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73504.1"
                     /translation="MKKIILFLVVITNFKAQTQPVYVDRIGFTLESIADIDIGWMKTY
                     KQTTPPKGKTAGNRVYSARQIGYTQQFIEWMQQSYLPKGCLGDIGHFQNAIPKFSGTN
                     SALGNAINQHLAALPHMYGAFSKVYMFLKKDAAGKFVPQNNFAEYWHIEANQIQYISA
                     PVSFISSPEQYYFVMPHYHSGAGSNFADGKAGYEFLGFSTHKNIQPYTRFYIPPKAIS
                     DYPHYIVIMTKNGELPFENVTIGEFFERAEEQLPAWQKVGNYTAENLAMARQNLARLK
                     EKYKKQLNEIATLGYPFGQIGFIDFVNARPNNRDFFDESEGSNKFPIQKVSKAAMELC
                     KTDQPQWITIRWTLGMPNSAYNVHMMESILNNFNFEYVYKYFFGSTKITESYKPLRSP
                     GHKEQAASEIASNEAKAAATDKNIFFFDDFSTNALNKPVNNWNSSLRNGKKAEVKQIA
                     GTNKKWMELQGRDASPKNIQYPLPQNFELSFDIAAPKDIPWGAKAFELYLGTKPTYDQ
                     MVSPAIKLRIKAGYWGRPGEISIQGKFADGYFANVKPYFEATGFSNDKELNTAKITLK
                     RKGESLELFMGENKIVEMSKAIPVTTTFNFLKFIHISSDSDLEKYYISNFKITKQ"
     gene            complement(27657..>28324)
                     /locus_tag="PLO70_03275"
     CDS             complement(27657..>28324)
                     /locus_tag="PLO70_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73505.1"
                     /translation="RFLCDEQSKDKTKYYVDYSTPTTVTITANAIFAQNLYAADLGDD
                     FRGYLKLTKKPEKKNGYYFMGEEKVDYNSPIYEYRWLITYNDSLPFSYLSRKEYLLIQ
                     KKRLEKAVKDNPGEKVYLDKYFKAIKEYMTRSETELSKPAICMWNNEERFEKFVDEGT
                     KGSFMAIKPNLDYYHKKLPKSTPQFFSVVYKISKDDAVFEENIEAIKKAVDFATLNNM
                     LGK"
     assembly_gap    28325..28334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28335..28636)
                     /locus_tag="PLO70_03280"
     CDS             complement(<28335..28636)
                     /locus_tag="PLO70_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73506.1"
                     /translation="MRYSILSAILFTSLTAFSQECSKELLKQKPGTWKAGSKGSVANV
                     STADLIKEKAITDGIHKMVSTNYKPMGCQISYSFVFGKSMASGQTWIADPYYYSMY"
     gene            complement(28650..30683)
                     /locus_tag="PLO70_03285"
     CDS             complement(28650..30683)
                     /locus_tag="PLO70_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73507.1"
                     /translation="MRQLLIFLIFISSLQLSAQPGKKPAETEKPPTQKEMADMMKEMQ
                     AAMDEMSPEDKKTMDSMGIKMPDIKSIKKNIAGISEAQLKKAYEDDNRIVPVKDVARI
                     NTALATTVSNAEMSTYINKTQQAVLSKLSSGTKIKGEEILQQIILLKSSVANTAVGLW
                     MDGKPTLALYLMGAACKTDPENTNNLNNYASFLTMCGAEQMALPILNNLNKRYAQNST
                     ILNNIAQAWLGLGDIPRAEKYADSTIRIHATHPQANMAKCLIEESKGNIPAAIAAAKK
                     SISEAYSNEKENKLKKLGYDLKSNDLNWDRPMPQDIMGLGKFTWPEYPMDVEQNKLLE
                     IEWKHFKEECQQKIDELKKKQKVLEVAYEKSSTERIRLVIQASQQGQFIQPIPGYAAK
                     AIKKLGPGINDVNGNMSFVFTRALEPVVRAALAAGEYEKILDKKQELLNKKYEDQIGE
                     GRPNPLEAICKDENAIRTEFLKNANGGIQSAYRQYLNYVSRTTSDLLYYCQYTMWPEQ
                     FELTKVNAQIAWLTQLKDQVVQFKNKSSWCIPIEVKPKGTDSLQNFDEVACQYVSKMN
                     LGVFKITSSCSDLVGEFDLKGVKINVKDNVETGKYSGTAIVGISESVGPMALKGKATI
                     SALVEWDNTGFTDIGAVAGVAVKAGPVTITGLDVKATVNSGVSTSGKGILGGK"
     gene            30844..32790
                     /locus_tag="PLO70_03290"
     CDS             30844..32790
                     /locus_tag="PLO70_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ73508.1"
                     /translation="MRKVFAVIISFCLVLTAVAQRSGPAHVLDSLLKVLATSKEDTSK
                     AMLLLTLANLYETNNQDSAEYYLQKGKALSEALKFDKGIYYYYQQGTVLSYTKGNYAK
                     ALDESNKGLEMARKLKDTLKVITMLNNLGIISAYQGNYQGQLEYALQVKDAVESVKDS
                     SMLSGAYHNLANCYHNLGQFRKAVETSGYSIYVNGLFSEKNMYINRVYATLGQSYEGL
                     RMADSALFYYSIAIKESVRLNDKYAEGSIYGYMCNLYAGMNRFGDMLKAAEKSLSLSK
                     ELQSRQMMASSLYNIAYAQFLNNNNKEAYKSIKEALSIAVADSLGDELKNCYTVLSYI
                     AARDGDYVTSVSAKQKADSIADASLNEKVLKSTADLEKKYETEKKDNQIKLQQAEIKR
                     KSTLNYILIGSAVALVVVSFLSYRNYRQKQKLQQQRIYELETEKKLAATEAVLKGEEQ
                     ERTRLAKDLHDGLGGMLSGIKYSFQTMKGNLIMTPENHQAFERSMDMLDSSIREMRRV
                     AHNMMPEALVKFGLDTALKDFCTDINQTGALNVSYQSIGMETSVIEQTTAITIYRIVQ
                     ELINNTMKHAAAKNAIVQVSRTNGILSVTVEDDGKGFDTAVLSQVRGIGWDNIKNRVE
                     FLKGTLDVDSQPGKGTSVHIELNT"
     gene            32791..33423
                     /locus_tag="PLO70_03295"
     CDS             32791..33423
                     /locus_tag="PLO70_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73509.1"
                     /translation="MAIKVFIVDDHYMVVEGIRSLLQNEKNVEWTGHAMNADSCLAFL
                     RQQQPDIILMDINLPDKSGIDLCKEVKEAYPQIFIIGLSTFNQQSFIQKMMDNGASGY
                     VLKNATQQELMEAIEMVVKGKTYLSDEVSRSLRRNEDDTIPVLTRREKEVLVLIAEGM
                     TNNEIAEKIFISASTVDTHRKNLLAKMPARNTAELVKLAFQHKFIKLDDK"
     gene            complement(33663..35861)
                     /locus_tag="PLO70_03300"
     CDS             complement(33663..35861)
                     /locus_tag="PLO70_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73510.1"
                     /translation="MLKIVFIATFLFSNILVQAQQPQKNQTAEMKQEIDNLKKEIKDL
                     ENEIKLLEQSDPDEAAQLKKELTALKSILSMMGGATAPAKQAVKPALKNSSQVKPAQS
                     PIVPIVLRQPVIVPTAAQAKDRLLWYKGKKMNDTTLVTMTGMVVQYAKKKSTVIVQPQ
                     KKTDRFEKTVNEIINNEKRKKDLAEDFEKMENGFLYYPLLVTSMAMYDDLAMGYASVV
                     KNTIELPELKPLPPGSEESPSAAINSAEKSHQTSAKEDAAKNANQLKDPGKQIEEQLA
                     LVKQLMQQLPAEESFPAPPARNLGICATCDTSLLAMQRRQDSIWVEAYQGREEKITSI
                     LLGIERMKSLLGQASNNSLADLLNPMTGRMDRKNKILLEKFGHDIRYTQIISTVVLGH
                     ERKRQLLGLAETEPPSLVQPLMKQAGDAYKKYFNEQVELKNHDFVLNMPFHLGVLRQQ
                     ALLGVEEGPSFGEFLNTFLEYNRFAMTTEIDFIYEQVNDNNEIELKATGTLESSVKKY
                     TMLIFDSCSYRMIPYTDNINNQTIEKVTMEMTVKAGTKTMRDEEGKLVTYKYTGPESF
                     PLQFPEFRIDFCNTSKPDTAFVTGFIGDEATAQQLNTAMSQINNGYKADILIYANYVF
                     YADEIDEEGAIDLGNEILESIAGMQNQAPATTAMGKLKQQYEGKKQMDNHRQGLINKL
                     SNQKTAFLFTANNKSTVFTDKYNDFKRTIEDNIELKKGLIHLRIVHEPVR"
     gene            complement(35940..37844)
                     /locus_tag="PLO70_03305"
     CDS             complement(35940..37844)
                     /locus_tag="PLO70_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73511.1"
                     /translation="MKKLFTCCFIFLGLLGHSQTNESFDIASFIAPKNWKKEIKSDVV
                     SYTITNQSNGGYCVIAVYKSIAGLGNIDKDYDKEWKELVVDRFTVNSTPETETEKRKD
                     GWLLKAGAAAINDGNGAAIAILSVFENSGSIMSVLALTNKEEFITNTENFISSIKFKK
                     IQGGLQEPTAPVATGNYKFTTSNFDDGWVSTVMNDYVLVEKEGNTVYLNYHVPYNASQ
                     FSGTGVRDAEYYWDTYVTKLFTVKSKQLNDGGSMALKPPYMEGYATDKRTGKPSFIGM
                     YLVIVPNAASVVIGTALNETAFRKLYPKANDPFGSDLAAMTRYNKFAVAPGDIVGKWQ
                     NGNTSTAQWYYTSPSGYEGYAGMTLAATSATFNFNKDGTYTSIHNGATGAVGNMNTFQ
                     QEYKGKYTVSNWAVTFTNRYGGKTDRFDAYFEAVRGGRILKLNNGAGQDYNLVKHSTN
                     TLPATSGNGTPSPSSTINADPKLFGKWNRSGASHPHYADPASWGTAGYTTSRYEFKKD
                     GSYQFTERSFRMTYQHIIIVKENGTYTVNGNQLTIVPAKSIIQSYTKKNNVDELGSLV
                     KSQNRTLEIVTYNYQFHYFSGIQEWNLVLQSSKPTQRDGNFSNNTTFSNAWYYDQKYT
                     DTDLTSPKGK"
     gene            complement(37943..38467)
                     /locus_tag="PLO70_03310"
     CDS             complement(37943..38467)
                     /locus_tag="PLO70_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73512.1"
                     /translation="MPNQLNNNTMRLLFLLVFFIPLIVCAQKDESFVKFINEDGSVIR
                     GSSVTKLYERQIPVFNLETNSSAYSTIVRFTLGTESAAGILRNLLQTNKKMRSGEIAV
                     TYTNYDRRQVRFKINMENIAVEECTDANGTTTVQLHATRIGWTYYSYTKSGVQSISSK
                     SGWDEEKRRAWTNF"
     gene            complement(38469..41522)
                     /locus_tag="PLO70_03315"
     CDS             complement(38469..41522)
                     /locus_tag="PLO70_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HQZ73513.1"
                     /translation="MKNVLLVLYGFIITCTVAQSQNCNPEEVTTYPGKWKPGMQGSIN
                     GVSAASLAKQKEIVGNFVKRLQQNMKLEGFNIDYSGVYGYPNPALVKNRKMDAYELSM
                     PVLPLYWENGQVKQAHETPYWVNIKINKIPYSGSQSFFVPKLIDEEDPDTDRLYYIDD
                     KPIKEISAWLLSENFIGGFGKELTRYKWVIPYTDQLPFRYLTRKELCTRLIDYYQKKA
                     RYTKIEDEINYNKKLVASAQDYLDKTPAEELNRDAKVRGSMLSVFYQYEDNVPPFYDD
                     ADKNSTYVIDIDPNYFKGSLPLHTPQIITLEFIVDEKNKAVMNNMYKMLDALDIENLK
                     TMMGNPTPFGKTNQPAKNTIPGTNTPGTVVEKKSASAKIQEKIISEPEAAALAQRANP
                     CPTENLSDYPGAWKPYSGYQAVAQFKAKPGSYNKSAAIANLDKLLALAKQAYHQPMGG
                     NANFTKYLDFSTAYSFMPIGYHLHIGHPGFVCGARNNIDLTYETGVTLKISINNPEVF
                     ASHITPYKVNGTDHYIGAMKNAESEYGINGKRVFLIHENFVSTNGWLDHYTEKIYRDE
                     EPTQQWFIIRRENEPLFRYVTRKEYLTQFREEIKEYRDVYIRHIENQYKKYPESFKQT
                     YEGLDDYKRRAENAIRLVDDYLKNKSREELSKPLSELINFEYILYSDATELKFRENRF
                     HLAFFNEEYLDKKLPLHIPQFIVVFLSGSAHDKTGPDSWKYNFRKKMMDGLDFKAMYN
                     MLAKIPVRVNQSQQPAAAKEIADKKSSPAVNPEKKSSPPELNKIEKPAIQKESPTTED
                     PLQPVYDLDGNKYTVIKIGNQYWLKENLRTTQYNDTTAIATGLTDSDWKQTKKGAYTV
                     YENNILNNNKYGKLYNGYAVATGKLCPKGWRVATDKDWKELEQFTGIPAAELDRTGER
                     GNIADKLKTTEGWKASVFSGTNSSGFSILPAGSRLDNGEFTTLGQFGNFWTSTVYDDR
                     YGLLYLWNHHANYNSNAIGRIYTLANNGYSCRCILDNNTTTKKTN"
     gene            41650..43617
                     /locus_tag="PLO70_03320"
     CDS             41650..43617
                     /locus_tag="PLO70_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ73514.1"
                     /translation="MKTKPFFMRAFHSLLILLFFSVPALAQQALADSVSKILQQKEIH
                     DTVKAYNLVMLAMYTEPLDINKAHTLYKEAVDFSLSRKLDYYAGMALYYQATPYHITG
                     QREKQFSNLQRAIALLEKLDSYKAKNELAGVYSSLSNYYRSAEKFDSAISASLRSIKI
                     QEDIKSYRRIVSSCLNLAMIYQQLKMPEKQKEYTEKGLTYAKQSGKNDALMLAYLQMA
                     SYYIELKDYSLAKNYADSATPYFDESYDFSRIQNFYLLKAGTFQNVKIYDSAVLYFKK
                     CYENASASNSRWNMTEPLMQIGYIYLQQKDYINAEKNLKLGLEIAETDSIRFFMKEGY
                     GTLSDVYAATGRYKEAFELLKKYNDIKDTLLNEDRKKFALELEKKYETQKKDDLLKNQ
                     QSEIAQRKRLNYALAGGAAVLVLTILLLFRNYKQKQRLQQQRIIELETKEKLAATEAV
                     LKGEEKERTRLAKDLHDGLGGMLSGIKYSFQTMKGNLIMTPENQQAFERSMDMLDSSI
                     KEMRRVSHNLMPEVLVRYGLDAALKDYTTEVNKSGIIKVVYQSMGMEQKMIEQSSALG
                     LYRIVQELLNNVIKHAAATEVLVQVFSEKDKLVVNVEDNGKGIDPAIMEKAEGMGWKN
                     IRSRVDLLNGKIDIQSAASKGTAVNLEFIKI"
     gene            43618..44244
                     /locus_tag="PLO70_03325"
     CDS             43618..44244
                     /locus_tag="PLO70_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73515.1"
                     /translation="MIKVFITDDHYMVVEGIRSLLINEKDIDFIGSASTVASCRAFLK
                     NQQPDVLLLDINLPDGNGIDLCKEIRELYPQIFILGLSTFNQASYIKDMMNNGASGYV
                     LKNATQKELTEAIHLASIGKTYMAYEVAKTLHTAEKQQSPEIVLGRREKDVLDLIKEG
                     FTNAEMADKLNLSVNTVDTYRKSLLAKLNAKNTADLVRLAFKYKLISD"
     gene            44355..44822
                     /locus_tag="PLO70_03330"
     CDS             44355..44822
                     /locus_tag="PLO70_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73516.1"
                     /translation="MRSILLFSGLFLIVTTVQAQDTIRRVQLPGNVNKKISGNRTIKK
                     VEPSIEQTIVKKINYVLDTFALQNNGDPFFIPKIKSVIETVLYPYLREGWLYGNEPRN
                     AWQIQCGSQTMTIKDKEDGRLVVFVSLALKKPSEFEVFRFERIVLNKQSLRYY"
     gene            complement(44827..46083)
                     /locus_tag="PLO70_03335"
     CDS             complement(44827..46083)
                     /locus_tag="PLO70_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQZ73517.1"
                     /translation="MKRFAPLLVFLLVLISGSAQQTEQKILSGADRINVYLPLIKGHT
                     VGIFANQTSMVGNTHLVDTLRKLGVDIKVIFGPEHGFRGTADAGEKVGNYIDDKTGIP
                     VISLYGSKRKPSAEDLKEVDLLIFDIQDVGVRFYTFISSLEEFMEAAFELGKPLMILD
                     RPNPNGHYVDGPVLDKKYKSFVGMQPVPIVYGMTMAEYAMMIAGERWLSEKANARHDY
                     YKTANNSVDTPFHFQVIKNANYTHKSKFVLPIKPSPNLPNIQSIYLYPSTCFFEGTVL
                     SEGRGTNKQFQVFGHPSLPKNLYSFTPNPNEGAKKSKLYGQVCYGWDLSGTPDEVSAK
                     VDNKVQLKWLIEAYKLFPQKDSFFIVPKSGNMEQSFFTKLAGNNELWQQVKAGKSEEE
                     IRKSWEPKLTVFKQIRKKYLLYEDFE"
     gene            46231..46686
                     /locus_tag="PLO70_03340"
     CDS             46231..46686
                     /locus_tag="PLO70_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQZ73518.1"
                     /translation="MSQVKSGDKVKVHYHGKLTSGETFDSSAGREPLEFEVGSGSVIP
                     GFDQGVTGMAIGEKKTINIPFDEAYGPRNPEMVIEMPKERFPQDMEIEVGMPIGMSDG
                     QGQQFQVTVVEIKEDVVMLDANHPLAGQDLIFDLELVEIVGGSPLIIMP"
     gene            complement(46795..47346)
                     /locus_tag="PLO70_03345"
     CDS             complement(46795..47346)
                     /locus_tag="PLO70_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73519.1"
                     /translation="MKKWLIGSLVGAILVFAWQFLSWTMLKLHEKEAKYTSGQDSVLT
                     YLSAALKEDGIYMLPTVPPGTPESEAMEMGKKMDGKPWATVMYKKAYTYDMVRPMIRG
                     FLVDLFLVFCLIYILTRAGTPTGMRIFAGSVALGLVTFLWGPYTAHNWFQTPTETLTG
                     HLIDSLAAWGLAGLWLGWWLNRK"
     gene            47455..48702
                     /gene="pepT"
                     /locus_tag="PLO70_03350"
     CDS             47455..48702
                     /gene="pepT"
                     /locus_tag="PLO70_03350"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQZ73520.1"
                     /translation="MFQNYKFTAADRFLRYVQIDTQSDPQSSSYPTTEKQKDLSRILA
                     EELKQMGITDAHMDEWGYVYATIPATSTKKVPVICFCAHVDTAPDCSGTNIKPIVHKN
                     YQGQDIILPDDKTQVLRMSEYPYLKKLTGHDIITASGTTLLGSDDKAGVAEIMDLANF
                     LMTNKQVKHGEIKILFTPDEEVGKGTAKVDLKKLGADFGYTLDGGDAGSLEDETFSAD
                     GVQVVIHGVIAHPGYAKDKMVNAMKIAGEILAFLPKDRLSPESTDGKRGFIHPVRVEG
                     IAEKCTIDFIIRDFDTEGLKKKEDYLRTQIEERMRTYPKASFEFTVTEQYRNMKEILD
                     VHTHVVDFAKEAIARAGLKLKMESIRGGTDGSRLSFMGLPCPNLFAGEQAIHSKLEFI
                     SVQDMNKAVETMVHLTCICEEKS"
     gene            48731..>49167
                     /locus_tag="PLO70_03355"
     CDS             48731..>49167
                     /locus_tag="PLO70_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQZ73521.1"
                     /translation="MDIKTWISQNWWVIVLVVVFFGSLFTINQGYIGVVTMFGKYRRI
                     VRPGLNIKIPILETVYRKVSIQNRSVELEFQAVTADQANVYFKSMLLYSVQNADEETI
                     KKVAFKFIADKDLMQALIRTIEGNIRSFVATKKQAEVLGLRRE"
BASE COUNT        13673 a         9542 c        10389 g        15553 t
ORIGIN      
        1 taaagctaaa aggatatatc tcacccgtgt ggaagcagaa cctgaggccg atactttttt
       61 cccatcacta gatcctcagc attggtacct ggtaagccag cgtaaccatg aagctgatga
      121 aaaaaatgca tacaattatt ctttccaggt ttgggagaga gtaggataac ttaagaactt
      181 tccttattca tgtattctgc cccgcaactc gccataaaat acctttgtta ttatttgacc
      241 gcttctaatg gtaagggcca tggcattcat tctcctttta tttttcaatt tatcacccgg
      301 gtgctgaatg atcatacaca gtacaaggaa tatgacaaag ttgaaaaatt acggcaacaa
      361 ttcttgaacg accggactat actaaccata caggattttg gagccgggtc atcggtatcg
      421 aagacaaacc agcgatcagt agcttctatt gcaaaaaatg cttccaagcc aaaaaaactt
      481 gcccagttgc tttttagaat ggtaaggctg tatcagcctt cttctatact ggaacttggt
      541 acttcgcttg gcattaccac tgcttattta tcactggcaa aacctgatgc caaactgttc
      601 accctggaag gggcagggga agtagcagcc cttgcagaaa aaaatttctt ggtttcaggc
      661 tttcaggata tttcagtggt gaaggggaat ttcgatgata ctttatctgt tgtactagag
      721 gggttgccga cagtctgctt tgcgtttatt gacggcaatc atcgaaaaga accaacagaa
      781 aaatattttt ctcagctgtt agcaaaaatt aataatgagt ctgtcctggt atttgatgac
      841 atacactgga gccgggaaat ggaacaggcc tgggagacga tcaggaatca tccttctgtc
      901 cgttgtacta ttgatctttt ttttattgga attgtctttt ttcgacagga gtttcatgaa
      961 aaacagcatt ttgttatccg ttattaagat gatgaataac atgcccctgg ctgaagaaaa
     1021 tcagtttctt cccttctgct gctccccaaa aaatcaatat cttcacacct atcggaaaat
     1081 ataaatttct ttctataatt cagtttctat atgacgattg gtgtattaaa agaacccgcc
     1141 cacgaaacga gagtttccct gctgtccgaa ttagtgcctt cgctgctcaa aaaaggggta
     1201 acagttctgg tagaaagcgg tgcaggtgaa aaggcatttt gcagtaatgc agcgtatgaa
     1261 aatgccgggg cacaaatcaa gagccgtagt gaagtgctgg cagctgctga tattgtgtta
     1321 agtatatttc agccagatcc ctcagaaatt gaccgcttga aatcaaaggt tatcattggt
     1381 gtatttcagc cattattcaa tgtggaagaa atgaaaagct gggcagtaaa agggctgacc
     1441 actttttcat tagacatgct tccccgtact acccgtgccc aggccatgga tgtgttgagc
     1501 tcccaggcaa atattgccgg gtataaagct gtcttaacag ctgccaatct ttattcccgt
     1561 tattttccca tgtttatgac agctgcggga agtataaaac ctgccaaagt actgatatta
     1621 ggtgccggtg tagcaggttt acaagctatt gccacagcca aaagattggg tgccgtcgtg
     1681 gaggtatttg ataccaggcc ggcggtaaaa gaagaagtga tgagccttgg agcaaaattc
     1741 gtggaggtag aaggagcggc agattccagt aaagccggtg gttatgcggt tgagcaaacc
     1801 gaagagtaca aacaaaaaca gcagcaaaaa attgcggata gtattgccaa ggctgatatc
     1861 gttattacta ctgcacaaat acccggtaaa aaagcaccca ttttgataac agaggaaatg
     1921 attaaagcaa tgcggaatgg ttccgtcatc atagatattg ctgctgctac cggaggaaac
     1981 actcctttta caaaaaacaa tgaaacggtg aattttaatg gggtgagtat tctgggtaat
     2041 tcctatttac catccaccat gccatcggat gccagtaaag tatatggcaa aaatgtctcg
     2101 atttttttac agttaataat tgataaggaa gggaaaataa atcttaattg ggaggatgac
     2161 cttgtaaaag gttgttgtat cacacacgaa ggagaagtga tacatgaaag agtaaaaggc
     2221 aattaatagt tgaataaatt atataggtat ggaaagtata ttaaaatgga tttatgccaa
     2281 tgagcagatg atctatatcc tggtattggt catctttctt ggatttatag ttattagcag
     2341 ggtgcctgcc gtgttgcata cgccattgat gagccatgcc aatgccatta gcggtgttgt
     2401 aataatgggt gctattattg taatgggcag agcagatgcc gataattatg ctgcgctggc
     2461 attagggctc ttagctgtga tacttggcac tatcaatgta gtaggcggct ttgtggttac
     2521 agaccggatg ctggaaatgt ttaaaaagaa aaaaaagtaa tgttagtcag caaaatactt
     2581 tttgattaaa ggtttaaatt attataaaca tgggagtttc agttttaagt atcatatata
     2641 tcattggtgc cgttactttt attgccggtt taaaaatgat gtctgatcct gcttcggcaa
     2701 ggaaggggaa tataattgcg ggtcttggta tgctggcggc aattatcgga accatttttt
     2761 tgtaccagaa tgaagatggc agcttgttaa agaatcatat ctggatattc gggggaatat
     2821 tgatcggatc agtcattggg gtgctggcgg caaaaaaagt gaagatgacg gccatgcctg
     2881 aaatggtgag tttgtttaat ggattagggg gtgctagtgc aacgttgatt tccctggtag
     2941 aatttaatca cttatctgat ttgaactttc cggctgaaag tgcaactccc ggaataatgg
     3001 caattctctt tgctggtatt atacttggaa atattacttt ttcaggaagt atgattgcct
     3061 ggggtaaact aagcggcagg gtgaaggatt ttgcatttaa aggtcagcat atcctgaata
     3121 tcttattact tgttatcagt attgctttat ccgtttatat aacttccaac ctccaggggg
     3181 ccaatacaac gatcctgttt tatacggtat ttctattgtc actggtatat ggaatctttt
     3241 ttgttttccc gatcgggggg gccgatatgc ccgttgtgat ctcgttattg aattcactaa
     3301 gtggtctgtc tgcagcctgc ggtggattcc tgtatgataa taaagttgtt tttaccggtg
     3361 gtatattggt tggggcagcc ggtactattc ttactgtttt gatgtgtaaa gccatgaacc
     3421 gttcactgac caatgttttg atcggatcat ttgggggcag taaagcggct ggtgataaaa
     3481 aagaacaggg ttcctataaa gaaatttcac tgaatgattc ggctgtcgta atgagttatg
     3541 ccagtaaggt aatgatcgtt ccggggtatg gtttggctgt ggcacaggca caacatacct
     3601 gtcatgagct acagaaaatg ctggaagcaa aaggggtgga gataaagtat gctatacacc
     3661 ccgtagcagg ccggatgccc ggtcatatga acgtactgct ggccgaagct gatgttagtt
     3721 atgacaaatt gattgaaatg gaacaagcca acgaagaatt tccggcggtg gatgtggtac
     3781 tcattttggg cgctaatgat gtggtgaatc ctgctgctaa atcggatcct tcttctccca
     3841 tttatggaat gccaatactg gatgtggggt tagctaagaa tgtaattgtg gtgaagagaa
     3901 gtatgaaacc aggatatgca ggtattgaaa atgatttatt ctttcagcct aagacttcaa
     3961 tgttgttcgg cgatgctaaa aaagtattgc aggatttggt aggagagatc aagagtttat
     4021 aagcatatgc ttccgggtta gctgatagtt catagttttg cgtcgtgcaa cttcctgcta
     4081 tattactgga tgctttacaa aatgccaggg gctttgataa aaatgccttt gaaagaattc
     4141 atgcttctgg ggagcaagtg acttcggtgc gtttaaaccc tttaaaaccg ccaaagggta
     4201 taactgatca tcccattcac caattaacaa gaataccctg gactgcatac ggattctacc
     4261 ttgaagaacg tccctccttt acgttcgatc ctttatttca tgccggtacg tattatgtgc
     4321 aggaagcatc aagtatgttc cttgagcaag cattaaaaca aaccgttgac ctgtcaaaac
     4381 cacttaaggt actagacctg tgtgcggccc cgggtggtaa gtccacgcat atccagtcaa
     4441 tgatttcaaa ggaaagcctg ctggtcagca atgaagtgat ccgttccagg gctaatgttt
     4501 taaaagataa tattatcaag tggggatgtg aaaatgtagt ggttacaaat aatgatccaa
     4561 gggatttcgc aaagacggag ggatactttg atgtgattgt ggtggatgca ccttgtagtg
     4621 gaagtggcct gtttcgccgt gatcctgatg caatagcaga gtggagtgag aataatgtac
     4681 aattgtgttc acagcgtcag caaagaatac tggcagatgt atggcctgct ttaaagaaga
     4741 acgggatgct gatctattcc acctgttctt actcgaaaga agaagacgaa gatattattc
     4801 aatggttatt gcaggaatta ccggttgccc tttgcccatt atcctttgaa ccatccggtg
     4861 gaattgtagc ttctggcgga ggctatcgtt tctggccaga taaagtaatg ggggagggtt
     4921 tttttattgc ctgctttcaa aaaaaagaag gggaagaaga attatcattc aaaataagga
     4981 acaggccgga gcagttaaat aaaaaggaga tggagatcgt ggagaaatgg attaagaatg
     5041 agggaaaggt attcatcaaa cacctgaata cggtttatgc atggcctgag ctgcttgaaa
     5101 atgaattcag ttttttatta gagaaactaa gggttgttta ttccgggata ctggttgggg
     5161 aattggtgcg ggataaactg gtgcctgaac atgcgctggc aatgagtaat agtgttgctg
     5221 attctataga aaatattgaa ttaactaaac aagaggcaat aaaatatttg cagcgtaaag
     5281 accttcaatt gcatacagca acaaagggtt ggcagctggc cagttacagc gggcatcctt
     5341 tgggttggtt gaatgtgttg gccaaccggg ttaataatta ctaccccaaa gaattgcgga
     5401 tattaaagga tatttaaaca gcttcaaaag gcaaaaaatc cacatctttg taatcaaatc
     5461 ttgtcggtat gaagaagttt gttctctctt tattaacctt atttatttct gtctctttta
     5521 tagtggccca gaaaggtgat ctgctggtga aaaatgggga taagggcttg tttcttgaac
     5581 ataaagtagt agcaaaggag agtttttttt cagttggccg tctgtataat gtccatccca
     5641 aatttatcgc ttcttttaac aacctggata tgggcaaagg gcttcagatt gatcaaaagc
     5701 ttcgtatacc ccttacggat actaatttta cacaaaaggg taatagcggc acacctgtct
     5761 actacaaagc ggatgttaat gaggggctga tgaaaataag taaagcaaac aacgatgtta
     5821 agttagcaaa cctgagggca tggaacaatc ttaccggaga taatgctggg gataaaaaga
     5881 aacttatagt gggtttttta ctgagtaaag aaatgccaac cattactatc ggcaccagtc
     5941 caaaaaccaa agagccaact gtaaaaaaag aagaaaaacc ggtggtggct gaaccaactg
     6001 tggaagataa gccagttgta aaaaatgaag aacccgtggt taaaaaagag gaaaagcctg
     6061 ttgtgacaga gccgaaagtg gatgataagc cagtagtgaa aacagaagag aagaccatac
     6121 caggcggcca gggttatttc aggtcacatt ttgagcagca gatcaggaaa tccccaatca
     6181 ctaaggatga aaccgtaact gccggtatct ttaaaacaac cagtggctgg caggatgaaa
     6241 aatattacat attaatagat gcagtagccc cgggaactat tatcaaaatt gtaaatccta
     6301 ctaatagtaa agctgtttac gccaaagtgt tgggagaaat gagtggtatc cgtcagaacc
     6361 agggatataa tatccgtatc agtaatgcag ctgctacggc attgcaggtg actgaacagg
     6421 ataagttcat cgtaaagatt aattactgat tactattaaa gcgataacct ctcaggaact
     6481 ccgtgatttc cattttcttc tttccttcca tttgaagttc cagtaagtga atatacccgt
     6541 cttcacaggc gaattttaag aaggtttttc cgtctgtttc ataatctcct tcggtcactt
     6601 ttgggaagat gatttctttc tttgctttga agattttgag catcttttcg ttcaggatcg
     6661 tgaaagccgc aggataaggg ctaagaccac gaatcaggtt gtaaattctt tcagccggta
     6721 agctccagtc tacccggcag gtggaagtat ttattttagg tgcatgcttt aaaaaggaac
     6781 tgtcaactgg catttgtttt tgacgatgag taactcttgt attgtcaatt gctggctgca
     6841 gatcatgaat tatgagatcc tgtgattttt cttgtaagct tccatcggcc aaccctttta
     6901 ctgtttttac caacagttgt gcaccgatgt ccttcatttt atcatgtact tctccggctg
     6961 tttcgtcctc accaattgca aaactttcct gcaataaaat atcaccggtg tcaatttcgt
     7021 gtttcaattt gaaagtggtt acgccggttt ctctttcacc attgatcaca gcccagttga
     7081 taggtgccgc accgcggtat tggggtagca aagacccatg aagattgaca gtgcccatgg
     7141 aaggcatatt ccagactatt tccggcaaca tccggaaggc aaccactatc tgcaggttgg
     7201 cgtttagtga ttttagttca gctataaatt ccttgttctt taatttttct ggttgcagaa
     7261 cttttaaatt gtgttccact gcgtacttct tgacagcact ttgctgtagc agcatacccc
     7321 tgccggcagg tttatcaggt gcagtaatga cgccaactat attataaccg gctttcacta
     7381 atgcatctaa agaggcaaca gcgaattcag gagtgcccat aaaaacaatc cttaaagaaa
     7441 ccagtgaaag attaaacgtg ttgggcccaa tatctttttg tttgatctgt tgttctatca
     7501 ttgatgcgaa ggtattgcaa agaagtaaaa gtattacgtg gatagcattg caatacaagg
     7561 aaaaattatg ttgaagtttt tgctttcaat aacttcaaca tcagaggcaa ctatcttttc
     7621 gccaggttaa ggttaccctg caaccaccca ttaatgcctg taaaacattc catttatagg
     7681 ataggggtgt atgcgccccc aacactttag cttttgcaaa actgatacct gcatctttgt
     7741 taatttcagt aaaaggtaac tggcccgaag ccagggtccg gttctcagaa ggtattaatg
     7801 aatgattttg tatccacaat ttggcttcct ggttcgaatt tatcataggt atgcctatct
     7861 ctttccacca acttactttt tttccattga tttcacctaa ttgaaaaaca aattccactt
     7921 tgtccctgtc aaccatatca tattctacta caaaggcaat ctcgtcacaa tcatcacctg
     7981 ttcttaatat gccgctggca gcaggaggtg gagtaggaaa cgggtagaac aagccagcat
     8041 actgtttatc tgtgggcgaa aaatccatgt tccatggaat actatcaccg gttgtagtaa
     8101 attcttttgg aacagggtac agcattatgg atttactgtc gaatgaagag tagttggtgg
     8161 aagtatagga gaatttagta aagatatttt gattcacctg ctccctggtc caataattgg
     8221 gtgctgcagc gtaatgggca tacacttttt ctttatccca cggaatggca gtaccaaaag
     8281 tttggtgttc gtgtacaaaa ccaagggcat ggccaaattc atgtaatatc acctgcctga
     8341 taaaaggtga ccctgcaggc aggctggtga gccaaccaaa attcattgtg gtttgtgtaa
     8401 caggtgcaag cagtgcatcc cggcctatca tagaatagga accggcacct tctttaaatc
     8461 ctacccttat ttttccgtcg gccaaattag ttacaaattc aattattata ttggcataca
     8521 attcccattc ttttgcatat tgttttacca ggtcgatgag gaagatgctg cctcctacaa
     8581 tatcaaaact caccgttatg gtttctcccg gctcccacat tttttccgta taagtggcta
     8641 atgactgacg gataaaagca aagggtgaaa cagttccgtc tgtatttatt ttatattgcg
     8701 caatatagtt tgtgggaata cgggctggca acggcctgct gcccacaggc ggaaaatcgg
     8761 tacaggtggt tgggtaactg aagttgaagt cttttttctt cactggaact atgtcctttt
     8821 tacgtacagg attaaaaaca agaagtgaat cattcttggt ggcgggattg ttattcaatt
     8881 cctgtttttt catggtccat ctgccccgct gtgtaaattt tgaattgctg cctgcatctt
     8941 ccagtctttg ccagtacccg cttatagtgt tgctatcttt ttttatatcg ccgccgcagg
     9001 catattgctg cacatcatta acgtatttat agacgatcct gacattatcc tcttttacag
     9061 aatatgtgcc ttctgccaga attttattat tctggtttac tacgttcatt tttcctcctg
     9121 gcaaaaattc gaacgagaag taataaggat tatccttctc attgttgcca taagtacctg
     9181 tccatcttcc ttccaataag aagttatgag gttgagcaaa cagtgtattg ctaaaaaaag
     9241 ccaggcagaa acagaaaatt atttttctca tatctattta tttttcgaat agcctgttcc
     9301 ggtattgcta ttcataggtt gttttaatat tgcccagata gaagacatca tttttattat
     9361 cgctgaattc acctttgtac ctgctgaaag tgtaaatatt gtaatgggct gttgttaaga
     9421 aaacatcccg cagatcggca atcgttttgc cattatcaat gaccactaat ccgtttcctt
     9481 ttctttgtaa ttgcagtttg tggttgtttt tatctttata aaaatccggc aatactatcc
     9541 gcttgttgaa tgccagggat tctcttttat cataggagta tacggtaagt gtacctcctc
     9601 cattccagta aggatcgaac caaaggataa cccggttaaa gttgccggca tatccattat
     9661 ataaactcca gaacatttcc tggttatcat ccaggcggta cctttcgccg gtattatcat
     9721 aatcaatatc tcctagcgtt actgtgaaag agccgctgtt ataagcaata tcttcattcc
     9781 attgcagatc aaaagataaa ttgaatttat cttttattgc tttgttatac tggcggggat
     9841 accaatagcc atctttagtg attgtaagcc atttgctgcc cttgtttgtt tgtacgatag
     9901 catttttcat tcccagccat cccatcggga attgcccatc tgccgtattg tcgaaactag
     9961 cgtttacagg ccctttgtct tcctgtttgg aatttgtttc ctttttaatt tctgtgacag
    10021 acgcattcag attatttatt ccatttggtt tttttgcagg ttctcccacc attttcgcca
    10081 gcacatccat gttgaactgg ctatggaata aatccataaa attctttttg ggtaagtcat
    10141 catcatccct tcttacaaaa aaagcgatac actgcggttc gtcttttggt ttagttttat
    10201 caaaaaaatc gagattatcc acccatgcat tatatccatt ttcgggagtt gccattaatc
    10261 tttccgggta aaaatgagat tcatttacac tggttacata agcaactgca ttaatggttg
    10321 actgctgcat ggctacatta taccaatttg atattttttc tagttcatct ttgtgctttt
    10381 gtaagatttc tttatttttt ctaatgattt cgggccaata attttgattt tgcttttctg
    10441 ctgctgaaag cccattgtat tttttctgat catccgctac taatttttca aagcggagta
    10501 cttcttgctt cactcttttt tcatgatgcg tcttgtacgc cgtaaaaagt tcactgttac
    10561 tcatttttct ccaaggcaaa tcttttcttc cggaaaaaaa atagcaatgt ttaattattt
    10621 catcaggccg gtactggctg tagattattt tattgccggt tgcatctgtc tgtacttcga
    10681 tattagtaac ccttatttta gattgattaa acgttatacc ggttccctgt tccggcagtg
    10741 gaaatttctc tccttctatc tgattcattg aaaaataagc ccaacagcca gttaccgctt
    10801 caggttttag ctcattgtat cctttcaggc attcaaattt tttcagcatc aggtaacatt
    10861 cataagcagc agggcttgtg gtactgtacc aaaatgcttt tcctttgggg aatgcaatac
    10921 tgttggtagc atgcaaacca tctaatgaaa gaggcatgat acgctgcatt tctttccgga
    10981 tggggtcact aaacttccat tggctggcgg gaatgggatc ctgaatagaa ggtgcaatat
    11041 tctgccatac ccatttgccg ggcactttgt ttacgtccag tgtgttacag tctgcttttt
    11101 cctgggcaat aagggtagaa gtaatcatgc aaaaaagtac aatcagtatg tatttcatct
    11161 gttgaactat tattttgttt tgaataaagt atatttcata gaactattat ccgagtcttc
    11221 catatacagc agaaaacctc cctttactgc aatgagttgg gctttgtatg ttgttgtttt
    11281 agccttgtag cggttggtga cagtaaggct ccagtcagta acagaaaaat ttcctttata
    11341 gtcctgtccg ccaaattgag caccaccact attcatactg gcactgcggt aggtgctgct
    11401 gtaagtaccg ttgctgttaa atgtaaattc atcagtagat gagacagcac tcatgcctgc
    11461 atacgtacca ctgtaaacat tataatattc cacgccaccg ccaccgcttc ccttccaggt
    11521 gccaataata tcattggcag tcagggcaaa ccggttggca ttcagcatag ggtctatatc
    11581 attgggatga ggaaactgct gctggtagtt gttttgatca ggagcaatga ccactatcgg
    11641 tcttgctcca ccactgtaca caattttaat agccacgaaa caggatttac cggttttctt
    11701 ttttaccgca tttgcttgta tgtggtaaat aacagggtac tgtacaccgc tccatttttg
    11761 tacgttacta acattgaaat agggctctac atatttgctc cagtaatatt ctggtgcatc
    11821 aatggtattg ggccgggcat catccaatgc tttgtctgta taatgcaggc gtagttccgt
    11881 gcctgctttg gtaagtttta cataatcatt gttgacggta gccgtccagc catcatcaaa
    11941 gctggtggtg gaattagtga tgccaccggt gttattattt gaaacaggaa tactgttttg
    12001 ctgagtagcc tgatttggtt ttcttaccat ttttctgcta tctgctttta tccccaatat
    12061 aaatttatcg ataactgcag catatttttc gtcattaaaa atagaaacgg cacaccagct
    12121 aatatcgttt tgagtaaaag tagcaactgc aacaataaat ggtattccat ccatggcagc
    12181 ctggcttgca ccggttacta cttcccaacc attttgtttt tctgtctggg tggtcggtgg
    12241 ttctgtaagg ttataaggtt tgacggcaaa atgttcccag tcggttttaa aatttgcagc
    12301 agggtctgat gaaccttgct gtgccgccca tatatgtatt tgacagaacg agttgccttc
    12361 tcgtttctca tagagcaggc gttctttttg ttcttttaaa acaaaaccgg cgggtgctga
    12421 gtatgaaaaa atatcatatt tctttatact ttgtccagct gattgcaaga ggattgaaaa
    12481 gaaaaatagt agtaagattg agaaatgttt catgatcggt cattatttta attcactttt
    12541 atttattctt cgtacgtaat taaaacttta aatggttttg aaaggatgtt tgcagaattc
    12601 cccggggtat taaaaaccca gatagaattg ttatagatgt cgtaattgta ttgaacaccc
    12661 gtaatatggg ttgatgaagg cggggtgtat atattagttt cagactccac caacacactt
    12721 acagataaaa tttttgagaa gaccaagccg tgtgggatat tagtgtctcc cccttctgtc
    12781 gaagcagttg tacctgttag tttttttact tttatggatg gggcatcact acccaacttc
    12841 gtaaaaccgt ttacttccag tttggtggtt gggctagtgg ttcccatacc aacatttccg
    12901 ttactggtat tcatcacaaa actccagccg ctgctatttc cataaaaacc cacctgctcg
    12961 ttactttgca ttccaataaa agctgggata tctgtattga gtgtattatt taaaaagata
    13021 cctgcactgt tattcaaatc gccaccgctg cggatacgca tacgaccact tacatcaagg
    13081 cggtagccgg gatcaatatt accaatgcct acattaccgt cattacgtac taaaaaacga
    13141 gatgtaccgc ctgtatagac ttctaaagca tttcgtgtgc tacctgcatc agctgttcta
    13201 acttctaatc cccagccagt ggcatcctgg ttctcgatac ttgcagcaaa aatgtttgct
    13261 gcggactttt gaacgtgaag tgggtatccc gggtttgtcg tgccaatgcc aacattgcca
    13321 ttgcgaagca cagttaaagc attgcttcgt atactaccgg aaccattgcc aatttgaaaa
    13381 atcctgtctg tgggtgctgt tagattaaca tctggattgt cggtattgtc attccagatg
    13441 ccagtagtaa aacttcctct tgcttttgca aatgtttgtt ctccggtact gaaggaatag
    13501 tttccacttg cagttgtgct tctgccagtg gcggtggaaa caagacctga tgcggtattg
    13561 ctttgcccca ttgctgtgga gtaatttccg ctggctatag tcgaagcgcc catactggtg
    13621 gctgaaaaac cactagcttc tgtaccgttg cccatactgg tagacacttc cccgctggca
    13681 taggtgccaa accccatact ggtagccact gagttaattg ctgaggtgta ttgtcccata
    13741 ctggtggaaa aacgaccgct tgcttttgtg ctatgccctg tagcaaatga aagtatgcct
    13801 atgttatccc tgtcccattc tgtgtccata acgtagccag ttctaaaagc acccaatgcc
    13861 ggaaaccaca ttgttctggt gccagcaccc tgcacaggcg gattgatagt ggtagtaaaa
    13921 ggcggaatgg aaacagtacc gctaagcaat acagcactgt cagtaacatg aaaccttgct
    13981 aacggtgtgg tagtgccaat gcctacattt tgagcagcag caataaaaat cattgcactg
    14041 tatatacatg acaggaagac tttctttttc atttttactg tttaatgatt ttatatgttg
    14101 ttgttccgtt tgttgtttgt aaacgcagca ggtataaacc cggtgacaat ttgtcaagct
    14161 taatggtgta cctgttgatt gatggaccaa ttttatcggt acgcagtatg ttgccattta
    14221 aatttatcaa ttgcagtgtt ttacttcctt cttcgccgaa atagactgtg gtctcgttat
    14281 gcactggatt gggcgcaata aatgtgttcg ttcgcaaatc acttttgtgt aaaacaatta
    14341 ttgtagagta agtaaacctg ccatccaaat caatttgttt aatgcggtaa taatttttac
    14401 caagggtagg agaggtatgc aaaaaatcat agcgttgcgt caggctgctg tttcctgcag
    14461 ctccaactgt tccaattttt gaataattga ttccattact gctgtgttca atttcaaaat
    14521 atttggtatt agtttcagaa gtggtggccc attttatctg tgctgcattg ctttgccatt
    14581 gaccatttat atagagccag gtaacaggta aaatgaaatt gcaggaaatt tgcaccggga
    14641 tattgcgttc cgcattagta ccatcaccta attggccggc atcattatta ccccatgccc
    14701 aaagcgaatt atcggttttt atagaaaagg tataatctgc tccgggggca atttgtcccc
    14761 agttagtagc cgtgccaatt tgtaccggag aatttctatt tgcattagta ccatcaccta
    14821 actggccacg aaaattccgg ccccatgccc ataaagtgcc atctgtttta gttgctattg
    14881 aatgttcctc tccggcagaa acgctttgcc agttggttgc tgcgcctatt agtgttggtt
    14941 gattttgatt agtgttggtt ccatcaccta atgcaccata attattatcg ccccatgccc
    15001 aaaggctgcc atttgttttt attgctatgc tgtggaacca tcctgcagca atttgccccc
    15061 aatcattatc aaaaccaatt gctgtaggaa cgtctttgtt ggtataagtg ccatcgccta
    15121 attggccatt caaattataa ccccaagacc acaaactgcc atctgatttg gtggcaatag
    15181 aattataagg tcctgtagta atgttttgcc agttggttgc tgttcctact tgcagcggtg
    15241 ttgccacact gttgggtggc aacgcattgc cacactggcc atactggttg ccaccccagg
    15301 cccataaact gccatctgtt tttattgcaa tgttatgttc agtccctgca cttatagtag
    15361 cccagtcagt agctgttcca atttgtattg gaacggtact attgttaata gtgccatctc
    15421 ccagttgtgc ttctgaattt cggccccagg cccaaagtgt tccgtctgtt ttaatggcca
    15481 tcgaatgttc tgctccggca gcaataactt gccagttagt agctgttcca atttgtccag
    15541 gagcattact gttgttattg gtgccattgc ctaattgtcc actggtatta tcaccccagc
    15601 cccaaagagt accatcagtt ttgattgcta atgtatgagc ataaccggca gatactttta
    15661 cccagcattg cgcattggtt tgaatagttg ccgtaacgag aataaacagg aggtaaaaag
    15721 tatttttcat gccttttgat ttaacggata caaagcttca attaatcagc aagggaaaca
    15781 atcccccaaa cacctgtatt tgccgtcccc tgttcagggg attttggata aaaacttgga
    15841 aatgtgctga atgttactct acttttaata ataatttcct gcaaaagcat tttagcttat
    15901 gaaaaggttt acccttttat ttttattgat gtgtgatcaa aatgtgtatg cgcaaaccga
    15961 tgcaaccagg gatagtatgt tgaacgtatt attggttgct aaagaagata ccaataaggt
    16021 gcatgcctta attaccacag ggcaatacgt ggagtataat aacctggaag aagcaaaaag
    16081 ttattattta caagcctatg agctgagcaa gaagttgaac tatacaatgg gtgttttaaa
    16141 gtattattct aactatacgg cagtgttgaa tatacaggcc aggtttgatt ctgctctcgt
    16201 tctcacaaaa tatgcattgc agttagcaaa caaatttgga aatgaagaac gtattataat
    16261 tgcacagcaa aatctttcag ccacctatgc ttacctgcag gattacgaaa gtgcattgca
    16321 gtatctgttg ccatcggtag cttatttcga aaaaacaaaa aatgatgccc ggttaagtct
    16381 tatatatgat aacctgggtg tgatatacag ggaaacaaag caatataata aggcattgga
    16441 gtatcatcaa aaggctctgg ccattgcccg taaggcggca aacagttatg atattgcaaa
    16501 cgtgttgtct aacctgggca attcatacag cagtttaaaa gaatatgata cggcacttgc
    16561 tgtaatgcag gaatccctgt tgattgctaa aaaagaaaat tatgataaca tagtatccaa
    16621 tgttacgagt aatattggat cgctattaat aaagcagggc aggtatgata gccttaattt
    16681 ttattgtgct gaaggtttac ggctggccga aaagttgggc gatagcctta ccatggtaaa
    16741 taatatttat ggcttggccc tggctgcatt ttatgacaaa gattttaaaa ggagcgaaga
    16801 agaagctgta gcaggctttg ctatggcaaa acaaagaaat tatgccgagc ctttacagct
    16861 ttttgcagat attttatcag aattatattt ggtaaaagga gatctgaaaa attatgacta
    16921 ttacaatcgg ttaagcatag aattagctga aaaatatttt agtgaaagaa tgcagcggaa
    16981 tgtgcaaaca ctggataaaa aatttgagac agaaaaaaag gaacagcagt tgcaattaca
    17041 acaagtcact atccggcaga aaagtacatt gaattatatt ttaattggca gtactgccgc
    17101 actgttgtta atttctttat tagtttatcg tactctcaaa caaaaaagaa aattacagga
    17161 gcaaaaaatt acccaactgg aaaaagaaaa attattactt gccactcagt ccatagtaaa
    17221 aggccaggag gaagaacgta acaggttagc ccaggacttg cacgatggtc tcggggggtt
    17281 gctgagtggt gttaaacttc aattaggagc tatgaaagga aatttaattt tatcagaaga
    17341 gcatggccgc acatttaata atgcgctggg caaattagat gaatccatca gcgaaatgcg
    17401 gcgggtggca cataatatga tgcccgaggc attattgaaa ttggggttgc aacaggcatt
    17461 gcaggattat tgcgacgggt tagccgaatc gcaacccttt acaatcaata cagagtttta
    17521 tggtttggag aaaagactgg aatcttcggt ggagattgta gtgtatcgta ttgtacagga
    17581 gttgctgaat aatgtggtaa agcattcagg tgctacctcc attcttgcac aggtaatgcg
    17641 gcaaggtaaa aatctcacta ttactgttga agataacggg aaaggctttg gggataatgt
    17701 atatactaat gaggagggag cagggttgaa gaatatccgg tcacgggtgg attacctgaa
    17761 agggcaatta gatataaaat caactttggg taaaggaaca tctgttcata tagactgttt
    17821 aattgaagat aatggataag atcaaagtac tggtggtgga tgatcacccg atggtgctgg
    17881 aaggtatgcg ttccatgctg gcgcaaatta gttttgtaga aatatccggc actgtatcga
    17941 atgcttttga tgccatagag atgataaaga tcaatctgcc ggatatcgtc attaccgata
    18001 ttaatatgcc agaagtcagt ggtattgacc tgaccgttaa gatcaggaaa gagtttcccg
    18061 ctgtgagggt agtggcgatg agtactttca aggaacgtag ctatatttcg cagatgatac
    18121 agagcggggc atccggttac ctggtaaaaa gcgcctcaaa agaggaaatt gaagaagcta
    18181 tactgagtgt gaacgaagga aaactataca tgagccttga tatcagcctt acctcatcgg
    18241 ataagcagga attaaataat atgcctgtac ttagcagcag ggaaaaagaa gtattgcagt
    18301 taattgctga tggcatgacc aatccgcaaa ttgcggcaaa gctattttta agcctccata
    18361 cggttgacag ccaccgaaaa aatctcctta caaagtttac agtaaacaat actgccagtt
    18421 tgattaaact ggcggcaaaa tataacctgg tgtagaagaa caaagcatgt ccaataagtt
    18481 agtggaggct ataccatgat gattttaact ttctttaaaa tcaatattcc gggtctcaaa
    18541 ccattcttta aactctcttc cttgttcacc ggcatagttg atacatattt ctgtaccggg
    18601 cggtatatct tccagggcat agtatgacat tgttcttgtc tccatgttaa ggctgtatgc
    18661 tgcattggag tatacagcat gattatataa ggagccaaag ccaagtacga gtgcaagggt
    18721 gccggcttcc ttgtcaaaat tgaaaaaata atctgccaaa tgcgatgaag taaccgtttc
    18781 ataatcccgg gcgggaagta cgagtactgc agctgtttca aatacctggt ctttacaaat
    18841 gcgttctttg caaaataccc cacggccttt accttttact tgctttatgt ataatgatgt
    18901 tttcattcag tgatgccggg ctgaaagtaa acaggagaat ggtcatctgc ttttccgtta
    18961 taattaatag ttatctcttc gcccggatgg atcattttgt tagcttttac aataagacat
    19021 ttcttgttta cagaaatagt gtaatcagca tttgcatcat aagaatgatt gtacagggaa
    19081 ctgtagccca ggccaagtgc aacgggagtt gtttcatctg ccaggaggaa ataatagtta
    19141 aataaaatag tggtttgtaa taactctctt tcctgccggt ctaataatat taccggagct
    19201 gtttctataa cagctccggg tttgaaagtc ctggttgcaa ataccccacg atcatcgata
    19261 gggctttgcc ttatctctat accggttggt tgcagtattt cctttggttt aaaaagggaa
    19321 agcattactc tttatgaggc tttgttgtag tctgaacatt ctctttcatt tgcacagtag
    19381 ttcccttttt aatgtggtca aacttaattg cttttttggt ttgcttgatt gtggtagccg
    19441 gctttactgt attgacagta tctgtaacga ccggtatagt tataaccggt ttaacggtct
    19501 ctttttttac agccggtgcc ggggtggtct gtgcttttaa tccggctaaa atcaataatg
    19561 aactgaatag ggcaagtgtt tttttcatat tcctgttatt tttaagttta aaaatttgtc
    19621 cattcttttc ctgaatcact gtcaaagcca tatttacggg aaactgtttg tctgccggta
    19681 gcatctgtct gataatatgt ccacccgatt cggccggcag taattacgga agtagttgtt
    19741 attacaccat tacagcccat agattcggca cagttgctta cacgaatgtt ttccatctta
    19801 atggtgtaca taataattgg agcaccggta ccaccaggtt gaagaaccga aagtgttccg
    19861 gacggtaaca gtgcgccatt acccatagcc ttttttaaat cggcagaagc accggttatg
    19921 ttcatactga aagaaagctg ggaattattt ttaccggcgc tggcaaatga caaaacgtca
    19981 atacttcttt cgtatccttt ggccagggca tctcccttaa tttgctggcc tgatgcatct
    20041 gtcagtttta aataaacagt ctggcctttt tgtgctaatg caacaaacgg gaaaaataat
    20101 attgctaaaa taattgtacg catagttttg tttttaagtg aatgatttat ttggctaatc
    20161 catattttgt gtaaatacct gatcccgggt aatctgcatc gagcaggttt aaaattcttg
    20221 ctccttttat gcaggaccag aaggcatgat aggttcttgc cctgtcttca atttttgaaa
    20281 atttaatctg ccagttattc agcaccgaaa aagtaccgga tgattttacc tggtcgaatt
    20341 tcatattgcc taccataccg tttacggcaa gcaatttcca gtgataggag ttgcctgcgt
    20401 taaaaataaa ttcttcattc gactggtgta tattcatacc ggcgtaattg ccggtgtaca
    20461 catggtacat ttgctggata cctgtaaaat cactggtcca ggttcctttt agatcggaag
    20521 cagcgaccgc aaacttattg cgtccaacca tgttggctaa aggtatcagg aggtccgttt
    20581 cgctgtccca ttgtattgtt tccgggtcaa atttgtattg ctgaataaaa gagtttttat
    20641 caggtgtaat aaactccagc catcccgttt cgccctgccg gaagaaaaca acatatactt
    20701 ctttgccagt tgcaatttct gtggcattgc ccatcccaag atagggtctg ttataagtgc
    20761 tgatataggt tgttttatag tttttgagat ctttgtatcg tggtgctaca agaatattcc
    20821 aggcgccatt cgttaagggt tcgggatcgg caggaaaaat ggtcccttct ttcggataat
    20881 gaaggagtac tttaatatct ccctttttta cttctaccca ctcttcctgc accgtactgg
    20941 tccatccgtc atcaaagttg gtggtgttaa aagcaaaccc atcttttttg gttgttgtat
    21001 tgggggctga tggctgtaca acagttttgg atggtttatc agcaggagtt tttgtttttt
    21061 taaagcttac tgattctaaa aaactggtca tttcggtttc atatgcatca gtgttggtaa
    21121 ggataaggat attgaccatt ttttggtaac cggtggaagt aaccagcact acaacaccct
    21181 tgctgtcctc gttctcaaat gctgcatagc cgctctgtgc ttcccagcca tcttcagtgg
    21241 aggagggttg catttctggg gctgttgaaa cggtcaccat ttcttttacc accgtttccc
    21301 aggctgcatc aaaattttct tttgaatcag ccgatcccgg gattgatttt agtactgtga
    21361 taatgcaata ggtgcctttt actgcatctt ctttggttag ctgtaccgca gtttcggtag
    21421 cctgtttttt ccaacccttt ggtggagtga aagttgttat atcaaatgtt tgctgctggg
    21481 caactattat tattggcgat acaataagcc agcagaatga atatattttt ttcatatcga
    21541 attgatttgc tattgtaaat ctattactct gccctcccga atcctgtttc acctatccat
    21601 aaaattcttg cattctttat acaagtgaaa tgcacagcag acgtttttgg ttttccttct
    21661 atgtctgaaa ggtttatttg ccagttaccg gacatagaaa acttgccatt tgacttgaca
    21721 ctttgaaatt taatgttgcc tacaaaacca ctggcaaccc caaggttcca tttgtatgta
    21781 ttgcctgccg caaactcaaa agattcatta gaagcatggg agtttgcgcc tgcactggcc
    21841 cctgtatatg catttacata ttgtgtcagc ccgaaaaaat cacttgtcca tttgccctgg
    21901 aggtcagtag ctgcgactgc aaatttgtta taattcgcca tcctttccat tttctcccag
    21961 ccataagatt cggggtggta tgcaccgaat tgctgttcaa aggaattttt atcgggagta
    22021 ataaattcta tatattttcc actgccgtta gaataattca ttttaaatag gactacatga
    22081 actgattttg cggtcaattt ttctaccata tctgcttctg caaattctat ggattgccag
    22141 ccggagattg gtttaaattc aaaattgccg gcactgctgt atcggggggc tactaaaaca
    22201 ttccaggcat tttttaaccc atccatcagg tttggattat atgcatctgt aattttattg
    22261 ggatagtgaa tcagtatttt agtgttttct ttaactactt ccacccagtc ttcctgtacg
    22321 gtgctggtcc atccatcatc aaagttggta gtgttgaaag cataaccatc attttttgat
    22381 actggttgaa ctggcttttt tgcagatgta ttttgtttag tggcagtggt agtcggtttc
    22441 ttaagactca ccgattctaa aaatgctgac atttcactct catatgcatc agaattggta
    22501 agcaccagca tggtaaccat tttttcatat cccgtagcag tgatcagcag ggcaacgcct
    22561 ttactgcctt cgttttcaaa agcggcatag ccactttgtg cttcccatcc gttttcttca
    22621 gatggagatt gcatttcagg tgctgcagac actgtaacca ttttttttac gactgactcc
    22681 caggcggcat caaagttggc ttttgagtcg gcatcggcgg gtaaggcttt tgatattgta
    22741 attatacaat agcctccagt tgttgcatct tctttgctta attgcagtac agacccggtt
    22801 gtttgctttt tccagccttt gggtgcagtg aaatgggtaa tatcaaagct ttgttgctga
    22861 gagtaggaac aaaagtttac tacaataagc aaaaaggata agcaatattt cattagtatt
    22921 attttaattc tgttactatc cggtccgcta tcaataacca tatctgaaat ttatttcaaa
    22981 agtgttattc gtttcattta attcaggaat atcattaata tgatctacag tcagataata
    23041 tttatattgt atgccattag caagtgtatt gagaacagag gaactaatag tgaattgatt
    23101 gtctattatg ccacctggat ttaatagggc accttcttgc aggagtttaa tatagctgct
    23161 tgacatttcg aggccattat ctttaactct gccgaatagg cggataccgt taatattaac
    23221 ttttacaggt gctgttcctg tattggtaat ggtataatta acaatataac cagtggatgt
    23281 ctttgaaatt gaagccctgg ttatgactaa gtctggcagt aattcctgaa gcggctgatt
    23341 tttaataata tttttcggtt tcatggtttg tgaaactggt tgttttatag ctttatttct
    23401 tctttgaaat aatgagtctg cattttgcgc aacactgaaa atcgcagcat gtagcataaa
    23461 agtgagaatg agcatctttt tcatagctaa tcttttttat taatgtaaat atgccataag
    23521 atggaaaagt ttcctatccc tgaaaaacag gatattttca gagtaataga aattttcgcc
    23581 aaagcaagac tttgtccata ttgctgaagt gtacgcaaca aacgaagaga ctagtactgc
    23641 agaccgtaaa attattataa ccctttgttg aaaaatatca caccctgtac agaagcttcg
    23701 gcaataccgc ctgcgcctgc accaaccgtg cctcttgctc cccaaccgca ttttgaatca
    23761 tagatgagtt caaatttctg attaattttt acagggctat cttttccaaa caagccggta
    23821 tccatgccta cgccaactga aaattcgtaa ctgccatcgg tattaaattc tgctccgccg
    23881 gatagcatac ctgcttccac accaacagat acgccagtag aaacttcttt aggactggct
    23941 aatttggtat cggattgctt gttcacttta ccatcggctc cttttgtata agtgttggag
    24001 ttgtcaataa aaatataatc tttagaactt atggcaattt tagtttttat agactctgta
    24061 gcaggacatt tttccggtgc cggtggttca aaagtggagc aggtgggtgt aatgctttca
    24121 tttgttttta caataagccc ggtgtagtga accttcttga taagcttatc attcagtgca
    24181 agcgattgat ctatataaat gaagggttct tttttatcac tcatagataa ctgtaaatga
    24241 aacggaccct tcatggcagg cccggcagca tctggtggga gaggactcca tggagttatt
    24301 ttctgccatt catttacatt gcgcagatct aacttttcag aaggcagacc ttgtatttct
    24361 acacggccat catcggtaac ggtgccggta aaattccatg atcttatttg aaatccatct
    24421 ttgatgtaag tggaaaatcc attgtaacta taggcagatg gcatcatacc ggctaccgca
    24481 tttgcagcgt tgccctggta tacatacata atttcaccgc tgccggttat atttccactg
    24541 gcagaaattt gcatggagaa atttttgacg caggccaatg tttgcaagtt gattatcgta
    24601 gttgccattc ctgctccaaa ttttgtattg gcatcagttg cattttctcc actgcgtgcc
    24661 gaaatatttt ttgcctggta atttccttgc cagtcagaaa gtttgtaacc agtaggaggt
    24721 ttttttgcta cgttaggttt tgctgcgttt ttagaagtag gatttgcgtt attagttttt
    24781 gcaggactgg cattgttcgc attgctatta ttggcctgag cttcacggcc attaccagca
    24841 taacctaaac tccatgcttt tgcagaaaga ttctcagctt cttttttatt ccctttttcc
    24901 tcagcaattt ttgcagcggt atagcaatac aatccattgt tgggtgctaa tcgcatggcc
    24961 atttgaatgg cttcatcagc ttcttttacg ttacccagtt tcaaatagat ataagcctgg
    25021 ttagcccagg gagaagcgag attggggaag ctttttgtaa gttcctctgt caatactttg
    25081 gcatcataaa ttctgcctaa ctctaataaa agattagaga agttatttaa gtaaagccca
    25141 ttggtttcct gtaattgcga tgcttttgca aagccatata gcgattccca tttgtagttc
    25201 ctgaaatagt atagcgttgc ctgtacatag tacgcctctg ctaagcctcc tttaagcgtt
    25261 ccggcacttt tgccggccct gctgtccaat tctttataga gttttgattt tataacggaa
    25321 cctgccagtg cattgtcaat aacctgtttt ttagtacttg tatttggtga aacaattgca
    25381 gtacttagcc aggtagaact tcctgcagca taattgagca ttttaaatgg tagcgtaagt
    25441 gtgccatttt gaaaagtgaa gttcccgctc ttctttccct gctccattgt tttaaaagtg
    25501 atggtgattt tgcttccgct tacctggtat gttccttcgt cggtaacaat attacccggc
    25561 tgttcagctt ttaacttgaa cgtattgtcg aggaattgaa gtgtgatgat cgcttttgca
    25621 cttgcttctt taccatcact atctgccata aaacgatatt taccttttat ggattggcta
    25681 tttacttccg tctgactggc acatacaata actgccagca ggaaaagaat atttttcata
    25741 tagattagtt gaagaagtgc tgtcttactg ttttgtgatc ttaaaattac ttatgtaata
    25801 tttctccagg tcgctatcac tgctaatgtg tataaacttt aagaaattaa aagtagttgt
    25861 taccggaatg gctttcgaca tttcaacaat tttattctca cccataaata gttccagcga
    25921 ttctcctttt cgttttaatg taatttttgc agtattcaat tctttgtcgt tggaaaatcc
    25981 ggtagcctca aagtagggtt ttacattagc aaaatagcca tcagcaaatt tgccttgaat
    26041 tgaaatttct ccgggtctgc cccagtagcc ggcttttatt ctcagtttta tagccggtga
    26101 taccatttga tcgtaggttg gtttcgttcc caggtacaat tcaaaagctt tagcacccca
    26161 gggaatatct ttcggtgctg caatatcaaa agataactcg aaattttgtg gcagagggta
    26221 ttgaatattt tttggagatg catcccgtcc ctgtagctcc atccattttt tattagtacc
    26281 ggctatttgc tttacttcag cttttttacc gtttcgcaag ctgctattcc agttattgac
    26341 tggtttgttt aaggcattgg tagaaaaatc gtcaaaaaag aaaatatttt tatcagtggc
    26401 agcggctttt gcttcgttgg atgctatttc agacgcagcc tgctctttgt gaccaggtga
    26461 acggagtggt ttgtatgatt ctgttatttt agtagagcca aagaagtatt tatatacata
    26521 ttcgaaatta aaattattga gaatactttc catcatatgt acattgtaag cactgtttgg
    26581 catccctaat gtccacctga tggtaatcca ttgaggctgg tcggttttgc aaagttccat
    26641 ggctgcttta cttacttttt gtatcggaaa tttgttgctc ccttccgatt cgtcaaaaaa
    26701 atccctattg tttggcctgg catttacaaa atctataaaa ccaatttgac caaagggata
    26761 gcctaatgtt gcaatttcat ttaattgttt tttatatttt tcttttaggc gagcaaggtt
    26821 ttgcctggcc attgccaggt tttctgctgt atagtttcca accttttgcc aagcgggtaa
    26881 ttgctcttct gctctttcaa aaaactctcc gatggtcaca ttttcaaatg ggagctctcc
    26941 atttttagtc ataataacaa tatagtgcgg atagtcgcta atggctttag gtggaatgta
    27001 gaaacgggta taaggctgaa tatttttatg tgtactaaac cctaaaaact cgtaacctgc
    27061 tttaccatct gcaaaattac tgcctgctcc actatgatag tgtggcataa caaaatagta
    27121 ttgctcaggc gaagaaataa aagatacagg ggcgctgatg tactgaatct gattggcctc
    27181 tatatgccag tactctgcaa aattattctg tggtacaaat tttccggcag catctttttt
    27241 taaaaacata taaacttttg agaatgcacc atacatgtgt ggaagtgctg ctaagtgctg
    27301 gtttattgca ttgccaaggg cactgttagt accactgaat tttggtatcg cattctgaaa
    27361 atggcctata tctcctaaac atccctttgg caaatatgat tgttgcatcc actctataaa
    27421 ttgctgggta tatcctattt gcctggcact atatactctg ttacccgccg tttttccctt
    27481 tggaggagta gtttgtttat atgttttcat ccagccaata tcgatatccg caatgctttc
    27541 gagtgtgaaa ccaatacggt caacataaac cggttgtgtt tgtgctttaa agtttgttat
    27601 aactaccagg aaaagaatta tttttttcat attgtaatta ttaaataacc agaaatttat
    27661 tttccaagca tattatttag tgttgcaaaa tcaacagctt tttttatcgc ttctatattt
    27721 tcttcaaata cggcatcatc tttggatatt ttatagacca cagagaaaaa ttgcggtgtt
    27781 gactttggta gttttttatg gtagtaatct aaattaggtt taatggccat aaaggaacct
    27841 ttggtgcctt catccacaaa tttttcaaat cgttcttcat tattccacat gcaaattgca
    27901 ggcttgctca attcggtttc agatcttgtc atgtattctt ttatagcttt aaaatattta
    27961 tcgagatata ccttttcgcc ggggttgtcc tttactgctt tctccaatct tttcttttgt
    28021 attaataaat attcttttct gctgagatag gagaaaggga gcgaatcatt ataggtaatt
    28081 aaccagcggt attcatatat cggtgagttg taatctactt tttcttcacc cataaaataa
    28141 taaccgttct ttttttcggg tttttttgtc aacttcagat atcccctgaa gtcgtcaccc
    28201 aggtcagcag cataaagatt ttgtgcgaaa attgcattag cggttatagt aactgtggtg
    28261 ggggttgaat aatcaacata atattttgtt ttgtccttac tctgctcatc gcataaaaac
    28321 cggannnnnn nnnnatatac attgaataat agtaaggatc ggctatccat gtttggcctg
    28381 aagccatact tttaccaaat acaaagctat aagaaatctg gcatcccata ggtttatagt
    28441 tagtagaaac cattttgtga ataccatccg ttatagcttt ttcctttatc agatctgcag
    28501 tacttacatt ggcaacagaa cctttactac cagctttcca tgtgcctggt ttttgtttta
    28561 gtaattcttt ggagcattcc tgtgaaaatg cagttagtga agtaaacagt attgctgata
    28621 aaatagaata tctcattttt taaagtttat tatttacccc ctagtattcc tttgcctgat
    28681 gtacttacac cgctattaac agtagctttt acatctaacc cggttattgt tacagggcct
    28741 gctttgacag ctacaccggc aacggctcct atatcagtaa agcctgtatt atcccattcg
    28801 accaaagcac tgatcgttgc tttccctttt aacgccatcg gtccgacact ttcgcttata
    28861 cccactattg ctgtaccgga atacttacct gtttccacat tatcttttac attaattttc
    28921 acacctttca aatcaaactc accaaccaga tcactgcagc tagaggtaat tttaaatacg
    28981 ccaagattca ttttgctaac atattggcaa gctacctcat caaaattttg taatgagtcc
    29041 gttcctttag gtttaacttc aatggggata caccagctac ttttattttt aaactgtact
    29101 acctggtctt ttagttgtgt gagccaggca atctgtgcat ttacttttgt cagttcaaat
    29161 tgttcaggcc acatggtgta ttgacaataa tataagaggt cgctggttgt gcggcttaca
    29221 taattaagat attgcctgta tgcactttgt attccgccat tggcattttt taaaaattcg
    29281 gtacggatag cattttcatc tttgcaaatt gcttccaatg ggttcggcct gccttctcca
    29341 atctggtctt cgtatttttt attcaacaat tcctgttttt tatccaatat cttttcatat
    29401 tctccggcag caagagcagc tcttactaca ggctctaatg ccctggtaaa tacaaacgac
    29461 atatttccat ttacatcgtt tattccgggg cctaattttt ttatggcttt agcagcataa
    29521 ccgggtatgg gctgaataaa ttgtccctgc tgtgaagctt gtattacaag cctgattcgt
    29581 tctgtactac ttttttcata ggcgacttcc aaaactttct gtttcttttt taattcgtct
    29641 attttttgct ggcactcttc tttaaaatgc ttccattcaa tttctaatag tttattttgt
    29701 tcaacgtcca ttggatattc aggccaggta aattttccca atcccattat atcctgtggc
    29761 atgggcctgt cccagttcag gtcattactt ttaagatcgt atcctaattt ttttaacttg
    29821 ttttcttttt cattgctata ggcttcactg atgctttttt ttgcggctgc aatagctgca
    29881 ggtatattcc ctttgctttc ttcaattaaa cattttgcca tattcgcctg cggatgtgtg
    29941 gcatgaatac ggatagtgct gtcagcatat ttttcagctc ttgggatatc gccaaggccc
    30001 agccaggctt gtgcaatatt atttagtatg gtgctgtttt gggcgtaacg tttattgaga
    30061 ttattcagaa ttggcagtgc catttgttct gcgccacaca tggttaaaaa cgaggcataa
    30121 ttgttcaggt tattggtgtt ttccggatcg gttttacagg ctgcacccat tagataaagc
    30181 gccagtgtag gtttgccatc catccacaga cctacagccg tatttgcaac cgatgatttc
    30241 aatagtatga tttgctgtag tatctcttcg ccttttattt ttgtgcctga agataattta
    30301 ctcaataccg cctgctgtgt tttattgatg taagtactca tttcagcatt agaaactgtt
    30361 gttgctaatg cagtattaat tcttgcaaca tcttttacag gaacgattct attgtcatcc
    30421 tcgtacgctt tttttaattg tgcttcactt atgcctgcta tgtttttttt aatacttttt
    30481 atgtcaggca tttttattcc catgctgtcc attgtttttt tgtcttccgg gctcatttca
    30541 tccatggcag cctgcatctc tttcatcata tcggccattt ctttttgggt aggtggtttt
    30601 tctgtttcag caggcttttt cccgggttga gctgatagtt gtaatgaaga aatgaaaatc
    30661 aggaaaatca gaagttgtcg catggggaat tattttatca aaagtgagta aaaggtaatg
    30721 tacaactcat ccctgaaaaa caggatattt tgttaaacga gtaaaatcat ggaaaaaggg
    30781 gagaagacct gctgaacttc tttgtttatt tgtaaagacg gactaatgat ttatcttact
    30841 agaatgagaa aagtatttgc tgtaattatt tctttttgtt tggtattgac agctgttgct
    30901 caacgcagcg gtccagccca tgttttggat agcctcttaa aagtactggc gacttccaaa
    30961 gaagatacaa gtaaagcaat gcttctgctt accctggcca acctgtatga aaccaataac
    31021 caggactctg cagaatatta ccttcaaaaa gggaaagcct tatcagaggc gttaaaattc
    31081 gataaaggta tttactacta ttaccagcag ggtaccgtgc tgtcctatac gaaagggaat
    31141 tacgctaaag cactggatga atccaataag gggctcgaaa tggcccggaa gctgaaggat
    31201 acactgaagg tgataacaat gctgaataac ctgggtatca tcagtgctta ccagggtaat
    31261 taccaggggc aactggaata cgcattacag gtaaaagatg ctgtggaatc tgtaaaggat
    31321 tcttcgatgt tgtccggggc ctatcataat ctggctaact gttatcataa cctgggacaa
    31381 ttcagaaaag cagtggagac atcaggttat tccatttatg taaatggtct tttcagtgaa
    31441 aaaaacatgt atataaaccg ggtctatgca acactggggc aaagttatga gggcctcagg
    31501 atggccgata gtgctttgtt ttattatagc atagcaatta aagaatctgt caggttaaat
    31561 gataaatatg cagagggatc tatctacggc tacatgtgca atttatatgc gggaatgaat
    31621 cgttttggtg atatgctcaa agctgcagaa aagtcattgt cgttgtcgaa agaattgcag
    31681 agcaggcaga tgatggccag ttcgctttat aatattgcct atgcgcagtt tcttaataac
    31741 aataataagg aagcttataa aagtattaag gaagcattgt ctattgctgt tgcagacagc
    31801 ctgggtgatg aactgaagaa ttgctatacg gtgctatctt atattgctgc cagggatgga
    31861 gactatgtta catccgtttc ggctaaacaa aaggcagact ctattgcgga tgcttcttta
    31921 aatgaaaaag tattaaaaag tactgccgat cttgaaaaaa aatatgaaac agaaaaaaag
    31981 gataaccaga taaaacttca gcaagctgaa ataaaacgga agagtacgtt gaactatatt
    32041 cttattggca gcgcagttgc attggtagtg gtttcctttt tgtcatacag aaactaccgg
    32101 caaaaacaaa aactgcagca gcaacgtatc tacgaactgg aaacagaaaa aaaactggct
    32161 gcaacagaag cagtattaaa aggagaagag caggagcgaa cccgcctggc aaaagacctg
    32221 catgatgggt tgggaggcat gttgtcaggc attaaatact catttcagac tatgaaagga
    32281 aaccttatca tgacacctga aaaccatcag gcttttgaac gaagcatgga tatgctggac
    32341 agttccatcc gggagatgcg ccgggtggcc cataatatga tgccggaggc attggtaaaa
    32401 tttggattgg atacggcatt aaaggacttt tgtactgata ttaaccagac aggagcattg
    32461 aatgtaagtt accaatccat cggaatggaa acatccgtta ttgagcaaac aaccgctatc
    32521 accatttatc gtatagtgca ggaattgatt aataatacca tgaaacatgc cgctgctaaa
    32581 aatgcaatag tacaggtaag cagaacaaat ggcatcttgt cggtaacggt agaggatgat
    32641 ggtaagggtt ttgatacagc tgtgctgagc caggtaagag gaataggctg ggataatata
    32701 aagaacaggg tggagtttct taaagggact cttgatgtgg attcacaacc gggtaaaggc
    32761 acctctgtac atatagaatt gaatacataa atggcaatta aagtttttat agtggacgat
    32821 cattatatgg tcgttgaagg gatacgttct ttactgcaga atgagaagaa tgtggaatgg
    32881 acaggccatg ccatgaatgc tgattcctgt cttgcttttc tccggcagca gcaaccggac
    32941 attatactaa tggatatcaa cctgcccgat aaaagtggta tcgatctgtg caaagaagtg
    33001 aaagaagcat atccgcagat ctttattatc gggctcagca ctttcaatca gcaaagcttc
    33061 atccagaaaa tgatggataa cggggcatcc gggtatgtac tcaagaatgc cacccagcag
    33121 gagttaatgg aagccattga gatggtagtg aaaggaaaaa catacctgag tgatgaggta
    33181 tcccgttctc tgcgaaggaa tgaagatgat acaatacctg tattaacaag aagggaaaaa
    33241 gaagtactgg tgctgattgc ggaaggaatg acaaataatg agatcgcgga aaaaatattt
    33301 atcagtgcca gtacggttga tacacaccgg aaaaacttac tggcaaaaat gccagcccgg
    33361 aatacagctg agctggtgaa acttgcattt cagcataagt ttataaagct ggatgacaag
    33421 tagctgcatt cattcttttt ttattgctgt caggttgaat tttcttcctg cgttgtaaca
    33481 accgaaatag tattgctacc ggtattcacc catgaatcta aacctgcttt tgaaaaccgg
    33541 ttgttcgcaa aaaaataaat cgcagcgatt gggtttgtgc tgtcgcagcg atttagttaa
    33601 tcaattacct tttactactt tatgtgccgg tgtattacat tcccatgtag ttcctgcctg
    33661 tattatctca ccggttcatg aactattctc aggtgtatca gacccttttt taattcgatg
    33721 ttatcctcaa tggttctttt aaaatcatta tacttatcgg tgaataccgt ggatttatta
    33781 tttgcggtga aaagaaaagc cgtcttttga ttggataatt tattgatcaa tccctgccgg
    33841 tgattatcca tttgcttttt cccttcatat tgttgtttca atttgcccat ggccgttgtc
    33901 gcaggcgcct ggttttgcat gccggcaata gattccagta tttcattgcc cagatcgatt
    33961 gccccttcct catcaatttc atcggcgtag aagacatagt tggcatagat taaaatgtca
    34021 gccttgtagc cattatttat ttgggacata gcggtattaa gttgttgagc agttgcttca
    34081 tccccaataa aacctgtcac gaaggctgta tcgggcttgg aagtattaca gaaatctatc
    34141 ctgaactcag ggaattgcaa gggaaaactt tcgggcccgg tatacttgta ggtcactaat
    34201 ttcccttctt catcccgcat tgttttggta cctgccttaa cagtcatttc catggtaact
    34261 ttttcgatcg tttggttgtt gatattgtcg gtatagggta tcatcctgta ggaacaactg
    34321 tcaaatatca acatcgtata tttctttact gaagattcca gggtaccggt agctttgagt
    34381 tcaatttcat tattatcatt tacctgttca tagatgaagt cgatctctgt ggtcatggca
    34441 aacctgttgt actccaggaa agtgtttaaa aattcgccga agctagggcc ttcctccaca
    34501 ccaagcaagg cctgttgccg caatacgcct aaatgaaaag gcatgttcaa gacaaaatcg
    34561 tggtttttaa gttcaacctg ctcattaaaa tattttttgt aagcatcgcc ggcctgtttc
    34621 atcaatggct gcacaagtga tggtggttcg gtctccgcca agcccagcaa ttgccgcttt
    34681 ctttcgtggc ccaacactac tgtggaaata atttgggtgt agcgtatgtc gtgtccaaat
    34741 ttttcaagca agatcttatt cttcctatcc attcttcctg tcattggatt caacagatca
    34801 gctaaagagt tgttacttgc ctgccccaac aggcttttca tcctttctat gccaagcagg
    34861 atgctggtta tcttttcttc ccggccctgg tatgcttcaa cccaaatgct gtcttgccgg
    34921 cgttgcatgg caagcaggct ggtatcgcag gtggcacaaa tacccaggtt ccgggccggt
    34981 ggagcaggaa atgattcctc cgccggcagt tgttgcataa gttgcttaac taatgcgagt
    35041 tgctcctcta tttgtttgcc cggatctttt agctggttgg catttttagc cgcatcttct
    35101 ttagcggaag tctgatgcga tttttctgcg gagttgattg ctgcggaagg agactcttca
    35161 ctaccgggcg ggagcggttt aagctcaggc agttctatgg tatttttaac tacacttgca
    35221 tagcccattg caaggtcgtc gtacatggcc atgctggtaa cgagcagggg atagtataaa
    35281 aatccgttct ccattttttc aaagtcttct gccagatctt tttttctttt ctcattattg
    35341 attatttcgt tcaccgtttt ttcaaagcga tctgtttttt tttggggctg cacaattact
    35401 gtgctcttct ttttggcata ttgtactacc atgccggtca ttgttacaag tgtggtgtcg
    35461 ttcatttttt tccctttgta ccaaagcaac ctgtcttttg cctgtgctgc tgtggggaca
    35521 atgactggct gcctcagcac gatgggtacg atcggtgatt gcgcaggctt tacctggctg
    35581 ctatttttca atgcaggttt taccgcttgt ttggccggag ccgtagctcc tcccatcatc
    35641 gacagtatac tttttaaggc agtcaattct ttttttagtt gtgctgcttc atcggggtct
    35701 gattgttcca acagtttaat ttcgttttcc aggtctttta tctctttttt caggttatca
    35761 atttcctgtt tcatctcagc cgtttgattt ttttgtggct gttgtgcctg caccaggata
    35821 ttactgaaga gaaaggtggc aatgaaaact atttttagca tactatattt tttatgatgg
    35881 atccaatttt ttaagggcat gaattattta ttttccggaa tttttattga aatcatttct
    35941 cattttcctt ttggtgaagt gagatcagta tctgtatatt tctgatcata ataccaggcg
    36001 tttgaaaaag tagtgttatt gctaaaatta ccatcccttt gtgtaggttt ggaagactgc
    36061 aaaacaaggt tccattcctg aataccggag aagtaatgga actggtaatt gtaagtcacg
    36121 atttctaatg tacggttctg cgattttaca agggaaccca actcatctac attatttttc
    36181 ttggtataac tctggataat actttttgca ggcacaatgg tcagctggtt accatttacc
    36241 gtgtacgttc cattttcttt tacgatgatg atatgctgat acgtcattct gaaacttctt
    36301 tcagtaaatt gataggaacc atccttttta aattcgtagc ggctggtggt gtagccggca
    36361 gttccccaac tggcagggtc ggcatagtga ggatgtgatg cacctgatcg gttccatttg
    36421 ccaaaaagtt ttggatcggc attgatagtt gatgagggtg atggtgtacc atttccgctg
    36481 gtggcaggta aagtattggt ggaatgtttt accaaattat aatcctggcc ggcgccattg
    36541 ttgagtttta aaattctgcc gccacggaca gcttcaaagt atgcatcaaa cctgtctgtt
    36601 ttgccgccgt accggttggt gaaagtaaca gcccagttgc tcaccgtata ctttccttta
    36661 tactcttgtt gaaatgtatt catattccca acggcgccgg tggcaccatt atgaatactg
    36721 gtataagtgc cgtctttatt gaaattaaaa gtggcgctgg tagcagccag tgtcataccg
    36781 gcataacctt catatcctga aggactggta taataccatt gagcagttga agtattcccg
    36841 ttttgccatt taccaacgat atcgccgggt gcaacagcaa acttattgta ccgggtcatt
    36901 gcggcaagat cagaaccaaa cgggtcgttt gctttgggat aaagctttcg aaacgccgtt
    36961 tcattgagtg cggtacctat caccacactt gcagcattgg gtactattac gaggtacata
    37021 ccgatgaaac tgggtttacc ggtgcgtttg tccgttgcat agccttccat ataaggaggt
    37081 tttaaagcca tgcttcctcc atcattcaat tgtttggatt tgacagtaaa caactttgtt
    37141 acgtaagtat cccaataata ctcagcatct cttacaccag ttccactgaa ctggctggca
    37201 ttatacggta catgatagtt gagataaacg gtattgcctt ccttttcaac cagtacataa
    37261 tcattcatga ctgtacttac ccatccgtca tcaaaattag aggtggtaaa cttatagtta
    37321 cctgttgcaa ccggtgcggt tggttcctgc aggccaccct ggattttttt aaattttatt
    37381 gagctgataa aattttctgt attcgtgatg aattcttctt tattggtgag tgccaataca
    37441 ctcattatgc tgccactatt ttcaaaaacg ctgaggatgg cgatggcagc accattgcca
    37501 tcattgattg cagcggctcc tgctttcaac aaccagccat cttttctttt ttctgtttct
    37561 gtttcgggag tgctgttaac cgtaaacctg tcaaccacca gttctttcca ttctttgtca
    37621 taatccttat cgatattgcc caggccagct atacttttat acaccgcaat aacacaataa
    37681 cctccgttgg actggttggt aatggtatat gaaacaacat cggattttat ctcttttttc
    37741 cagtttttag gtgcaataaa agaggctata tcaaagcttt cattggtttg gctgtggccc
    37801 agcaaaccca aaaagatgaa acaacaagta aataattttt tcatatccaa tgtttattgc
    37861 gctgttttat taacccgcat cattattccg ttaatcagct acttctttat ttctttaatt
    37921 atttatcaaa agaactgccg tatcagaaat tggtccatgc ccttcttttt tcttcatccc
    37981 agccgctttt actgcttatg gactgtaccc cgctttttgt ataagagtag taggtccagc
    38041 cgatccgggt ggcatgtaat tgaacggtgg tggtgccatt cgcatcagta cattcttcca
    38101 ccgcaatatt ctccatattg attttaaatc ttacctggcg gcggtcataa ttggtatagg
    38161 ttacggcaat ttcaccggaa cgcatttttt tattagtctg gagcaaattt cgcagtatgc
    38221 ctgcagcact ttccgtgccc aaagtaaaac ggactattgt gctgtaagca gaagagtttg
    38281 tctccaggtt gaatactggt atttgtcttt cataaagttt cgtaaccgaa gaacccctga
    38341 tcaccgatcc gtcttcgtta ataaatttta caaagctttc atctttctgc gcacaaacaa
    38401 ttaacgggat gaagaaaaca agtaagaata gtaatcgcat ggtattgttg tttagttgat
    38461 ttggcatttt aattagtctt ttttgttgtg gtattgttgt cgaggataca cctacaggaa
    38521 taaccattat tggccagggt ataaattctg cctatggcat tgctgttgta attggcatga
    38581 tggttccata aataaagtaa accgtaacgg tcatcataca cagtggaagt ccagaagttt
    38641 ccaaattgac ccagcgtagt gaattctcca ttatccagtc ttgaaccagc cggtagtata
    38701 gaaaatccac tcgaattggt accggagaaa acggaggcct tccatccttc ggtggttttt
    38761 aatttatctg cgatattgcc cctttcaccg gtgcggtcaa gttctgcagc aggaatgccg
    38821 gtaaattgtt ccagttcttt ccagtctttg tcggtggcta cacgccagcc tttggggcat
    38881 agtttgccgg tagcaactgc atagccattg tatagcttgc catatttatt attattcaaa
    38941 atattattct catacacggt ataggctcct tttttggttt gcttccagtc gctgtcagta
    39001 agacctgtgg caatagctgt cgtatcgttg tattgggtag tgcggaggtt ttcttttaac
    39061 caatattggt tgcctatttt aattactgtg tatttgttgc cgtcaagatc atagacgggc
    39121 tgcaacgggt cctccgtagt tggtgattct ttttgtattg ccggcttttc aatcttattt
    39181 agttcgggcg gagaggattt cttttcaggg ttaacagcag gagaagattt tttatcagct
    39241 atttcctttg cggctgccgg ttgctgtgat tggtttaccc ggaccggaat ttttgccagc
    39301 atattataca tggctttaaa atccaaccca tccatcattt ttttcctgaa gttgtatttc
    39361 cacgaatccg gtcccgtttt gtcatgggct gaaccggaaa ggaagacaac aataaattga
    39421 gggatgtgaa gtggtaattt tttatccagg tattcttcat taaaaaatgc caggtgaaaa
    39481 cgattctccc ggaattttaa ttcagtagca tcagaataaa ggatgtattc aaagttaatc
    39541 agttcagaaa gcggcttact tagctcttct ctgcttttat ttttcaggta atcatctacc
    39601 agcctgatgg cgttttctgc ccttctttta taatcatcaa gaccttcgta tgtctgctta
    39661 aaactttccg gatacttttt gtactgattt tcgatatggc gtatgtaaac atcccggtat
    39721 tctttgatct cttccctgaa ttgtgtgagg tactctttgc gggtgacata cctgaacagg
    39781 ggttcgtttt ctctccggat gatgaaccat tgttgtgttg gttcttcgtc tctgtagatt
    39841 ttttccgtgt agtggtccag ccagccattg gtgctgacaa aattctcatg tatcaggaaa
    39901 acccttttac cattaatgcc gtattctgac tcagcattct tcatggcacc tatataatgg
    39961 tcggttccgt ttaccttata gggagtaata tgagaagcaa agacctccgg attattgatg
    40021 gaaattttga gggttacccc tgtttcataa gtaaggtcaa tattattcct ggctccacaa
    40081 acaaacccgg gatgaccaat gtgcaggtgg tatccaatcg gcatgaatga gtaggcggtc
    40141 gagaaatcca ggtatttggt aaaattggca ttgcctccca ttggctgatg gtaggcttgt
    40201 ttggcaaggg ccagcaattt atccaaattg gcaatggctg cagatttgtt ataactaccg
    40261 ggtttggctt taaattgtgc aacagcctgg tatccactgt aaggtttcca tgcaccggga
    40321 taatcactca ggttttctgt aggacagggg ttggctcttt gtgccagtgc ggcggcttca
    40381 ggttcactta ttattttttc ctggattttg gccgacgctg attttttttc aactacagtg
    40441 ccaggtgtat tggttccggg gattgtattt tttgcaggtt gattggtttt accaaaggga
    40501 gtgggattac ccatcattgt ttttaggttt tcaatatcta aagcatctaa catcttatac
    40561 atattattca tcactgcttt atttttttca tctactataa attcaagggt gatgatttgt
    40621 ggggtatgca ggggcaggga gcctttgaaa tagttgggat ctatatctat cacataggtg
    40681 ctatttttat ctgcgtcgtc ataaaacggc ggaacattat cctcgtactg gtaaaaaacg
    40741 gagagcatac tgccccgaac tttcgcatcc cgattcagtt cttcggcagg tgttttatcc
    40801 aggtaatcct gtgcactggc aaccagtttt ttattgtagt ttatttcatc ttctattttg
    40861 gtatacctgg cttttttttg ataataatca atcaacctgg tgcacaactc tttgcgggta
    40921 agataccgga aaggcaattg atcagtataa ggaatgaccc acttgtacct ggtcagttct
    40981 tttccaaagc caccaataaa attttcggag agtaaccaag cggatatttc tttgatgggt
    41041 ttatcatcaa tgtagtaaag cctgtccgtg tcgggatctt cttcatcaat aagttttgga
    41101 acaaaaaaac tttggctccc ggagtaagga attttgttga tcttgatatt tacccagtag
    41161 ggagtctcgt gggcctgctt cacctgccca ttttcccagt aaagcggaag taccggcatg
    41221 cttagctcat aagcatccat tttacggttt ttgacaaggg cagggttggg gtagccatag
    41281 acacctgaat agtcaatgtt aaaaccttcc agcttcatat tttgctgcaa ccgctttaca
    41341 aaattgccca caatttcttt ttgtttggcc aggctggctg ccgacacgcc attgatactg
    41401 ccctgcatac cgggtttcca tttacctggg taggttgtta cctcttccgg gttgcagttt
    41461 tgagattgtg caactgtaca agttataata aacccatata aaactaatag gacatttttc
    41521 attttctcac ttaattttcc tcaaagcttc tttttttatg tagtttgcta tatcccgtaa
    41581 aaagagatat ttattctgcc gggatcactg aaaaagggga gtcgattaaa taaataattc
    41641 atttcatttg tgaaaacgaa accattcttc atgcgggcat tccattcttt gctcatactg
    41701 ttattctttt cggtaccggc tttggctcag caggcactgg cagatagtgt gagtaagatc
    41761 ctgcagcaaa aagaaataca cgatacggta aaagcctaca acctggttat gctggccatg
    41821 tataccgagc ccttagatat caataaagca cataccctat ataaggaagc tgttgatttt
    41881 tctctttcca ggaaactgga ttattatgcc ggaatggcat tgtattacca ggccactcct
    41941 tatcatatca ccggtcagag ggagaagcaa ttcagcaatt tgcaaagggc tattgcattg
    42001 ctggaaaaac tggatagcta taaagcaaaa aatgaattgg ccggagttta tagttcactt
    42061 tccaattatt accggagcgc cgaaaaattt gattcagcca tatcggcttc attgcgatcc
    42121 attaaaattc aggaagatat taaaagttac cggcggattg tttccagttg cctgaacctg
    42181 gccatgatct atcagcaatt gaaaatgccc gaaaagcaga aggagtatac agaaaagggg
    42241 ctgacttatg caaaacaatc cgggaaaaat gatgccctga tgctggccta tttacaaatg
    42301 gccagttatt atattgaact aaaagattat tcccttgcaa aaaactatgc ggattctgcc
    42361 actccctatt ttgatgagag ctatgatttt tcccgcatac aaaatttcta tcttttaaag
    42421 gccggtacat ttcagaatgt aaaaatatat gacagcgctg tcttgtattt taaaaaatgt
    42481 tatgaaaatg ccagtgcttc aaattcccgc tggaatatga cagagccgct tatgcagatc
    42541 ggttacattt acctgcagca aaaagattac atcaatgctg aaaaaaacct gaagctggga
    42601 ctggaaatag ccgaaacaga ttcaatacga tttttcatga aagaaggata cggaacgcta
    42661 agtgatgtat atgcagcaac cgggagatat aaggaagcct ttgaactgct gaaaaaatat
    42721 aatgatatca aagatacttt gctgaatgag gatcggaaga agtttgcact ggaactggaa
    42781 aagaaatatg aaacacagaa aaaagatgat ctcctgaaaa atcagcagtc agaaatagcg
    42841 caacgtaagc gtttgaatta tgcgttagcc ggcggagctg cagtactggt attgaccata
    42901 ttattactct ttcgaaatta taagcaaaag caacggctgc agcaacagcg tatcatagaa
    42961 ctggaaacca aagaaaaatt agccgccact gaagcagttt tgaaaggaga ggaaaaggaa
    43021 cgaacccgcc tggcaaaaga cctgcacgat ggattgggag gtatgttgtc gggcattaaa
    43081 tattcatttc aaactatgaa aggaaatctt atcatgacgc ctgagaacca gcaggctttt
    43141 gaacgcagca tggatatgct ggacagttct attaaggaaa tgcgccgggt atcacataat
    43201 ttaatgcctg aagtactggt tcgatatgga ttggatgcag cgctaaaaga ttacacaacc
    43261 gaggtaaata aaagcggcat tattaaagtg gtttaccagt caatgggaat ggagcaaaaa
    43321 atgatcgaac aatcttcagc acttggattg taccggattg tacaagaact gctcaataat
    43381 gttataaaac atgctgcggc aaccgaggta ttggtgcagg tgttttccga aaaagataaa
    43441 ctggttgtaa atgtggaaga taatggcaaa ggaatagacc cggccataat ggaaaaagct
    43501 gaaggaatgg gctggaaaaa tattcgttca agagtggatc tgctcaacgg gaaaatagat
    43561 attcagtctg ctgccagcaa aggcacagca gttaatcttg aatttataaa aatataaatg
    43621 ataaaagtat ttatcacaga cgaccattac atggtggtgg aaggaatccg ttcattgctg
    43681 atcaatgaaa aggatattga ttttatcggc tcagcttcca ctgttgcttc ttgcagggct
    43741 tttttaaaaa atcagcaacc ggatgtattg ttattggata taaacttacc cgatggtaat
    43801 ggcatagatc tctgcaagga aatccgggaa ctgtatccgc aaatattcat cctgggacta
    43861 agtactttta accaggccag ttatataaaa gacatgatga ataatggagc atctggttat
    43921 gtgctgaaaa atgctacaca aaaggaactt actgaagcta ttcatttggc ttctatcggt
    43981 aaaacttata tggcctacga agtggcaaaa acattacata cagctgaaaa gcaacaatcc
    44041 cctgaaattg tattaggcag aagggaaaaa gatgtactgg atttaataaa agaaggcttt
    44101 accaatgctg agatggcaga taagctaaat cttagtgtca atacagtgga tacctaccgg
    44161 aaaagcctgt tagcaaaact caatgcaaaa aatacggctg accttgtacg cctggccttc
    44221 aagtataaac tgatttcgga ttagtagtat tcaccgggca tttgaaggca agatttagtc
    44281 ctggtaacaa caattctttg ctgttttgct aatgaattgg tttaagattt ttaaataata
    44341 ggtaaaaaat atttatgcgt agcatacttt tatttagcgg tctgttcctt attgtaacta
    44401 ctgtgcaggc ccaggatacg attcgcaggg tacagctgcc cggcaatgtc aataaaaaaa
    44461 tcagcgggaa caggacgatt aaaaaggtag aaccatccat tgaacaaact atagtgaaga
    44521 agatcaatta tgtgctggat acattcgcct tgcaaaataa cggtgacccc tttttcattc
    44581 ccaaaataaa aagtgtaatt gaaacagtgt tgtatcctta cctgcgggaa ggatggctat
    44641 atggtaacga gcccaggaat gcatggcaaa ttcaatgtgg cagccaaaca atgaccatca
    44701 aagacaaaga agatggccgg ctggttgttt tcgtcagcct ggccttaaag aagccgtcgg
    44761 aatttgaggt cttccggttt gagcggattg tattgaacaa acaatccctg cgttactact
    44821 gagcggctat tcaaaatctt cgtagagcaa atattttttc ctgatctgtt tgaatacagt
    44881 gagtttgggt tcccagcttt tgcggatctc ttcttctgac ttaccggcct ttacctgttg
    44941 ccacagttca ttattaccgg ccagcttcgt aaaaaatgat tgttccatat ttcctgattt
    45001 gggaacaata aagaaactat ctttttgcgg aaagagtttg tacgcttcga tcagccattt
    45061 taattgtact ttattatcca ctttcgccga tacttcatcc ggcgtaccac tcaggtccca
    45121 gccataacaa acctgtccgt atagtttgga tttttttgct ccttcatttg ggttgggtgt
    45181 gaaagaataa agattctttg gcagtgatgg atggccgaac acctggaact gtttgttggt
    45241 acctctacct tcacttaata cagtgccttc aaagaaacat gtagaagggt acagatagat
    45301 ggattggata ttaggaagat tgggagaggg ttttataggc aaaacgaatt tgcttttgtg
    45361 tgtatagttg gcatttttta tgacctggaa atgaaaaggg gtgtcaacag aattattcgc
    45421 tgttttataa tagtcatgcc tggcattggc tttttcactc aaccatcttt caccggcgat
    45481 catcatcgca tattcagcca tggtcatacc gtatacgatg ggtactggtt gcatgcctac
    45541 aaatgattta tactttttat ccagtacagg accatccaca taatgtccgt tgggattggg
    45601 tctgtccagt atcattagtg gttttccgag ttcaaatgca gcttccataa attcctccag
    45661 cgaagaaata aaggtgtaga accgtacccc tacatcctgg atgtcaaaaa tcagcaagtc
    45721 cacctccttc agatcttcgg ctgatggttt ccgtttgctg ccatataagg agatgacagg
    45781 aatcccggtt ttatcatcaa tatagttccc tactttttcg ccggcatccg cagtgccgcg
    45841 gaagccatgt tccgggccaa atatgacttt gatatcaaca cctaattttc ttaatgtatc
    45901 tactaagtga gtattaccga ccatcgaagt ctggttagca aatatgccaa cggtatgtcc
    45961 tttgatcaat ggcaggtata catttatcct gtctgcacca gacaggatct tctgctctgt
    46021 ttgttgagct gacccggaaa tgagtacaag gagaaaaacc agtaatgggg caaatcgttt
    46081 catggaagtt tttttaatgt tgaaatctgc agtaaaatac tgtaaaatat taatacagac
    46141 tgtcttgcat atttaaacgg ttgcattgac ggatttggcc taacttgcac cccggtttta
    46201 tttaaaaaaa caaacattaa aacaaaaagt atgtcccagg taaaaagcgg tgataaagta
    46261 aaagtgcact atcatggtaa attaacttcc ggtgaaactt ttgattcttc agcgggtcgt
    46321 gaacctcttg aatttgaagt aggaagtgga agcgtcatcc ccggttttga ccagggtgtt
    46381 accggaatgg caataggaga gaagaagacc atcaatatac cttttgatga agcctatggt
    46441 ccccggaatc ctgaaatggt gatcgaaatg ccgaaagaaa gatttccgca ggatatggaa
    46501 attgaagtag gtatgccaat agggatgagt gacggacaag gtcagcagtt ccaggtaacg
    46561 gttgtagaaa tcaaggaaga tgtggtgatg ctggatgcca atcatccact ggccggacaa
    46621 gacctgatct ttgacctgga gttggttgaa attgttggcg gaagcccgtt aattattatg
    46681 ccataaacga accggcaaca agcaacaggt aaatatgtac caggccatgc ctggaataaa
    46741 aaagttcata gtacacacta tgaacttttt ttatagtatc attttattat tgttctattt
    46801 tctattcagc caccagccta accacaatcc tgccagtccc catgctgcca gtgaatcaat
    46861 caaatgcccg gtcaacgttt cggttggtgt ttgaaaccag ttatgtgctg tataaggacc
    46921 ccataaaaaa gtaacaaggc ctaaagcaac cgaaccggcg aaaattctca taccggtggg
    46981 ggtgccagct cttgttaata tatagatcag gcaaaacacc aggaacaggt cgacaaggaa
    47041 tccgcggatc atcgggcgga ccatatcata agtgtaagct tttttataca taacggtggc
    47101 ccatggctta ccatccattt ttttgcccat ttccattgct tccgattcag gagtgcccgg
    47161 tggaactgta gggagcatgt aaataccatc ctcttttaat gcagcactta aataagtcaa
    47221 tacactgtcc tgacccgaag tgtattttgc ttccttttcg tgcaacttta gcattgtcca
    47281 tgacagaaat tgccatgcaa aaacgagaat agccccgacc agggagccga ttaaccattt
    47341 tttcatgttg ttggttttag tttaagtata aaagatagac atattctttc tttttgtcaa
    47401 gtgtttttct cttgccctca tttagtcgta tattcgatgc ccttaattac aagtatgttc
    47461 cagaattata aatttaccgc agcagacagg tttctgcgtt atgtgcagat cgatacacaa
    47521 agtgatcccc aatcttcttc ttacccgact acggaaaagc aaaaggattt aagcaggata
    47581 ttggcagaag aattaaaaca aatgggcatc acagatgcac acatggatga atggggctat
    47641 gtgtatgcca ctattcccgc aaccagcact aaaaaagtac cggtgatctg tttttgcgcc
    47701 catgtggata cggcaccgga ttgcagcggg acaaatatta aaccgatcgt tcataaaaat
    47761 taccagggac aggatattat attgcccgat gataaaacac aggtactccg aatgagtgag
    47821 tatccttacc tgaaaaaatt aaccgggcat gatatcatta ctgcttccgg aacgacattg
    47881 cttggctctg atgataaggc cggggtggca gaaataatgg acctggctaa tttcctcatg
    47941 actaacaagc aggtgaagca tggagaaata aagatccttt ttactccgga tgaagaagtg
    48001 ggtaaaggaa cggctaaagt agatttaaaa aaattaggtg ccgattttgg ttatacactt
    48061 gatgggggag atgccggctc gctggaagat gaaacattca gtgcggatgg tgtgcaggtc
    48121 gttattcacg gtgtgattgc acatccgggt tatgcaaaag ataagatggt aaatgccatg
    48181 aagattgcgg gtgaaatact ggctttttta ccgaaggacc ggctttcccc ggaatcaaca
    48241 gatgggaaaa gaggttttat acacccggta agggtagaag gcattgcaga aaaatgtacg
    48301 atcgatttca tcatccgtga ttttgataca gagggtttaa aaaagaaaga agattacctg
    48361 cgaacgcaga ttgaagagcg gatgcgaact tatcccaaag cctcttttga gtttaccgta
    48421 accgagcaat accgcaatat gaaggaaata ctggatgtgc atacacatgt ggttgatttt
    48481 gccaaagaag cgattgccag ggcaggactg aagttgaaaa tggaaagcat ccgtgggggt
    48541 acagatggca gccgtttgtc ttttatggga ctgccttgcc ccaacctgtt tgccggagaa
    48601 caggccatac attctaaact ggagtttatc agtgtgcagg acatgaataa agcggtggaa
    48661 acaatggtgc atttaacctg catctgcgaa gagaaaagtt aatagtaact tgcatcataa
    48721 attttaaatt atggatatta aaacttggat ttcacagaat tggtgggtca ttgttctggt
    48781 cgttgtattc tttggttctt tgtttaccat taaccagggt tatatcgggg tagtaacgat
    48841 gtttggtaaa taccgtcgta ttgttcgtcc gggactgaat attaaaattc ctatactgga
    48901 aaccgtatac cggaaagtat ctatacagaa ccgttcggtg gaactggagt tccaggcagt
    48961 aacggctgac caggcgaatg tgtatttcaa aagcatgctg ttgtattctg tgcagaatgc
    49021 agatgaagaa acgatcaaga aagtagcgtt caagttcatc gccgataaag acctgatgca
    49081 ggcattgatc aggaccattg aaggaaatat ccgttcattt gtggccacaa agaaacaggc
    49141 cgaagtgctc ggacttcgcc gggagat
//
LOCUS       DAORTI010000013        48343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_418_length_48343_cov_8.248716, whole genome shotgun sequence.
ACCESSION   DAORTI010000013 DAORTI010000000
VERSION     DAORTI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 48343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48343
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_48343_cov_8.248716"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLO70_03360"
     CDS             complement(<1..972)
                     /locus_tag="PLO70_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQZ73522.1"
                     /translation="MKILIISLLLVNSCLLINAQLYQSTMNDDSSRIIELREITISTN
                     QKNHQRLLVNFFRSNNSSTLEEIISRLPEVSLLRRGSYGMEPTLRSFNGGQINVLVDG
                     MRIHGACTDKMDPATIYIEPINLENLQVQTVTTGFMSGSSVGGTINMKMAEPDYLNAN
                     KITGMISSGYQTAAKSFYESARLNYSTDKWAFRMSGTYRNNKNYRSSGGDVIDFSHFE
                     KANYSLSVKYKHNTYTYFKADLLGDDGWNIGYPALPMDVGYAAARIGSLSMHRENPSK
                     SLYKWQVKIYANKIDHTMDDTKRPNVPIHMDMPGWSKTYGAYSEAELK"
     gene            complement(1023..1853)
                     /locus_tag="PLO70_03365"
     CDS             complement(1023..1853)
                     /locus_tag="PLO70_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQZ73523.1"
                     /translation="MKKSILILLSLAVLCVAVFLSACSKDDVQEPDPVAGLTKIIEGY
                     AAGAATKVEVYTKEASINAGYTKFYIALYDSVSGNRIEDAHIQLTPIMDMGTMIHSSP
                     YENPASEKTINHLFPCSVTFIMSSMGGTWTLKIQVQNNLTDKEGSLTIPITVNEPTRS
                     RQKSFTAVHNGAKYFISLIEPTAASSKVGINDMEIAIYKKVSMMSWPADSSLSVILTP
                     EMPTMGHGSPNNVNPTHIGNGHYKGKVNFTMTGLWRLNLDFMSGAAVADTTQYFDVEF
                     "
     gene            complement(1951..2325)
                     /locus_tag="PLO70_03370"
     CDS             complement(1951..2325)
                     /locus_tag="PLO70_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73524.1"
                     /translation="MKKVIAIILVFIFSFQLFYATGFTIWFFANRTTIAKEYCINKDR
                     PELKCDGKCFLNNKIKETEQHQNEEAPLQIKQLLETSPCTITTINYSISAPEIDLIYN
                     PFSTATYSFNHTIAVFRPPSLS"
     gene            complement(2397..3020)
                     /locus_tag="PLO70_03375"
     CDS             complement(2397..3020)
                     /locus_tag="PLO70_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ73525.1"
                     /translation="MTISLSDAGKRFNRDWIFRHFTYTFETGNAYAITGPNGSGKSTL
                     LQVISGGMMINEGKMQWAITGNELANEQIYQHVSICAPYLEVIEEMTLTEFMNFHQGF
                     KPWLPGLSAKEIISIIGLEKAVHKQIRYYSSGMKQRVKLAQCILSDTMIVLLDEPCTN
                     LDTAGIDLYHSLINKYCKHRLVAVSSNDETEYKFCTHLININDYKRH"
     gene            complement(3001..3807)
                     /gene="lpxA"
                     /locus_tag="PLO70_03380"
     CDS             complement(3001..3807)
                     /gene="lpxA"
                     /locus_tag="PLO70_03380"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQZ73526.1"
                     /translation="MIHPHTYIHPNARLATNVKIDPFTVIHQDVEIGDGTWIGSNVTI
                     MEGARIGKNCRIFPGAVIAAIPQDLKFQGEYSNVYIGDGTTIREFVTVNRGTKDRDKT
                     VIGKNCLIMAYCHFAHDCFVGDNCITSNNTQVAGHVTIGDWAIIGGMCAVHQFVTIGA
                     HSFISGGSLVGKDVPPYIKAGRTPLSYAGVNSIGLKRRGFSVERINHILDIYRVLYNK
                     GMNTSQAVEYIEEEMPATDERDEIVTFIRESGRGIIKRFTKGSNDDDLPI"
     gene            3944..4912
                     /locus_tag="PLO70_03385"
     CDS             3944..4912
                     /locus_tag="PLO70_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ73527.1"
                     /translation="MIKRNFAVRMHNPPDILTLAYKQILMQDQMVLLQEKEQQTPGSV
                     QYTINRYRRHPQWNIEDTGMLVYHYEKKEPKENYLELKFCVSGNVYCRKKDSECDMCQ
                     FNASKTCMERVESVDVVSFRFSPVHMSQFVKPRKGNAALTDDILSFSHNSSFTKILPL
                     CGKTRMVLEGVLNHTYSDSLENIYINAQTQMLLLYSLDCMLGEKEIDVISCKFLANEA
                     DREKIVKAREILIQHIGEPITIKELSRKVAINECYLKKGFKELFGTTVFDFYQSQRME
                     HAKYLLYEKGLSVTEVSMMLGYSSISHFSTAFKKHTGLKPCELLLR"
     gene            5060..5440
                     /locus_tag="PLO70_03390"
     CDS             5060..5440
                     /locus_tag="PLO70_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73528.1"
                     /translation="MRKTLAILLISVHLVGNTEAGQLFKLPQLLQHFFQHHHLDPSIN
                     FIEFMAMHYGGDDGTSADDDFDQELPWHNLNQHSIAVVYFPMIKEIASTEFSYGETKV
                     YASHLPPAISAKHALLILQPPRLG"
     gene            5587..6306
                     /locus_tag="PLO70_03395"
     CDS             5587..6306
                     /locus_tag="PLO70_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73529.1"
                     /translation="MKNKIVYVIFSLIAGLFILNACRHEILNPGNGSGGGGTGGGGNL
                     PPTSICNPDSVYFVNDIMPLISSNCTMSGCHDNITHADGVNLTTYTKIMGYVRPGDAA
                     NSKLYKVIIKTDGDRMPPPPMAPMTTAQKALIQKWINQGAKNNSCIGSCDTTVFTYSG
                     AVKPIMDNKCAGCHNPSNLGGNIDVSTFAAVRVVALNGKLYGSIAHQTGYSPMPKNAA
                     KLSDCEIRQVQKWIIAGSQNN"
     gene            6317..6703
                     /locus_tag="PLO70_03400"
     CDS             6317..6703
                     /locus_tag="PLO70_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73530.1"
                     /translation="MNIKFSILGILIVSVLFVSSCYYDKEDLLYGEANSAPCTDTTGV
                     ISYTQKIVPMLQQYCYSCHTGSFPSGNQVMGTYATDKAMAQNGKLYGTINHAAGYSPM
                     PKGMAKMSNCQIATIKKWVDSGMLNN"
     gene            6719..7174
                     /locus_tag="PLO70_03405"
     CDS             6719..7174
                     /locus_tag="PLO70_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73531.1"
                     /translation="MKRKKIILYVLLSVLVIGGAVGIYIYKEYNRAHKDTAGLKPDFT
                     VAANDLLKEFENNEQASNKKYWDKVLSVEGMVKDVTRDDRGFYSVILGDTSSLSSVRC
                     SIDSIHGNEAASVKRGHIVSVKGICSGYNADDMLGSDIILVRSVIGQKK"
     gene            7201..7761
                     /locus_tag="PLO70_03410"
     CDS             7201..7761
                     /locus_tag="PLO70_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQZ73532.1"
                     /translation="MKNHLLLATVLMLVATSSFGQKFFTKSGKIDFDATSPASPEKIE
                     GVNRTATCVVDTKTGAIQFAVLMKGFAFERALMEEHFNENYVESHKFPKAEFKGSIIN
                     NETINYAKDGTYSVTVKGKLTIHGETKEVETPGKLTVQGGKINANASFSILLSDYKVS
                     IPGLVADKVSKTAKLIISCSLEPLKS"
     gene            7780..8688
                     /locus_tag="PLO70_03415"
     CDS             7780..8688
                     /locus_tag="PLO70_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HQZ73533.1"
                     /translation="MKKLSSQFVTGVVLLLFSSNLFAQDEDLLKLVGGDEKPTKERVK
                     YAFKSPRVINGHSMEFLNPGTMDFRILHRFGQLGQGYKNFFGLDQASMRMGFDFGILQ
                     NLMVGVGRSTYKKEVDAFIKYAPVRQSTGPWSSPVTFALVSGITMDGLPWADPNRKNF
                     FTSRLAYYFQAIIGRKFSEGLTLQVSPTMVHKNLVQLQTDPNNIYAIGVGGRLKLSQR
                     IAFTWDYSHVIIGMPDSGYYHPLSVGFDIETGGHVFQLHFSNSTGMNERAFITETTGK
                     WGKAEVRFGFNLSRVFQIKKNKKANE"
     gene            8771..9352
                     /locus_tag="PLO70_03420"
     CDS             8771..9352
                     /locus_tag="PLO70_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQZ73534.1"
                     /translation="MIAELFNQLARSRRSVFPDQFEAGKKVDDAIIKEILTNATWAPN
                     HGKTEPWQFTVFSGEGLNKLAAFQSELYKELAGDSFKELTYTKLQQNPLKASHIVAIG
                     MKRTTNKNIPEVEDIEAVACAVQNMYLSVTAYGLGGYWTTGGVTYKEEAKSFFGLGEL
                     DKLLGFFYIGYIAIPSKGATRLPLEEKVNWIKE"
     gene            9356..9787
                     /locus_tag="PLO70_03425"
     CDS             9356..9787
                     /locus_tag="PLO70_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQZ73535.1"
                     /translation="MPKQPVTPPYHIYYPVKVAKSKIAGKGAYALQTIPAKKKIGDLG
                     GVIITMKEAMKLIKNLKVINLVELDNDLALNASAKPNDMRFINHSCDPNTYLRVMKNR
                     VEFYALKKIKKGQELSCDYGETHHEGTLPCRCGAKNCRGFI"
     gene            9851..10903
                     /locus_tag="PLO70_03430"
     CDS             9851..10903
                     /locus_tag="PLO70_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQZ73536.1"
                     /translation="MKKYSIISLVSALVLFCISFTNENLRSIYSRPPAEWPKPFVDDG
                     VQWKELGMLPESPVEKYKDSLMHIIKLGKTLFFDTRLSGSGKISCATCHQPELNWTDA
                     KEKSIGHEGAINKRNSPTIQNSWFYNRLFWDGRSADLQDQAFAPINSETEMHSEMPDV
                     IRKLRKVNGYKELFKKAFGDEKIDPDRLTEAIAVFEKTIVSRKSRFDEFLEGSKKALT
                     NSEIRGLHLFRTKAKCMNCHSGPLLTDNLFHNSGFSGTDEGYYKITHLEEDRGKFKTP
                     SLRDVMKTGPWMHDGKQNNMLQIIEKFNTGDMPVGKDKLIQPLGLTKREKTDLLAFLQ
                     AISAPPLEFQKPVLPD"
     gene            10969..11445
                     /locus_tag="PLO70_03435"
     CDS             10969..11445
                     /locus_tag="PLO70_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HQZ73537.1"
                     /translation="MKKMFFTLVMAGFGFIVNAQPPAGDAKPGEWYGEKISTEGALNI
                     NDVVAKLNGGSEFPEVKIKAKITEVCPKKGCWLKLELENGETAMVKMKDYGFFLPVSA
                     KGKTVVIDGKVEIKTTSVAELKHYAEDAKKSKEEIDAITKPEKEVRVMAKGIVIAE"
     gene            11610..13823
                     /locus_tag="PLO70_03440"
     CDS             11610..13823
                     /locus_tag="PLO70_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73538.1"
                     /translation="MLRRMMSIVAVMVIAPVIFAQDSTTIFHDDSTKPRSKYLADVTV
                     VGRNSKSDYQQMPEIVGTSIYAGKKNALIVLDNVQGNVVTNTMRQVLAKVPGIHVWES
                     DPSGIQIGIAARGLSPNRSWEFNIRQNGYDIAADPFGYPEAYYNPQLQAVQRIEVVRG
                     QGALQYGPQFGGLVNYILRNGSEISKKFEFETQQTVGSNALINSYNAIGGKSGKWHYY
                     SFFDHRNGDGWRQNSRYFTNAGYGTATYYFNPKLSLTAEVMFSHIRSQQPGGLTDLQI
                     DLDAQQSFRSRNWFDIKWTTPALILNYQLNESTKWNTKLFVTIGNRSSVGFLQSITVK
                     DSINSGTGEYNNRTVQVDNYRNYGLESRIITDYKIGKLQNTLSGGIRLYTGTTHRMAD
                     GNGTTGSSYDVSIIGNYPRDINFDSKNAAAFAENIFRFSDKFYIIPGIRLEWLEGSAS
                     GRNGYTSGGVEILLQNINRSRSFVLAGIGTEYHITRTTEIYANISQAYRPIQFANLQA
                     PPTTDVVENDLKDAKGFNADLGYRGKVKNYLQFDISGYYLHYNNRLGTVTVSGTPSYR
                     LITNVGSSTSKGFEGYAEFNPWRAFTQNTNADIIVFGSYSYTDARYSSDHKDAATKGK
                     KVENAPQNIFRGGITAGYKTFLLTTQLSYVGEAFSDANNTLAPTANGNNGLIPSYTVT
                     DITATYKFSKGLNIKTGINNLTDARYFTRRAGGYPGPGALPADGRTFFVSIGAKL"
     gene            14041..16098
                     /locus_tag="PLO70_03445"
     CDS             14041..16098
                     /locus_tag="PLO70_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73539.1"
                     /translation="MRKIHLFLVAVLINASVHGQLVKIAPPEDTTKAKELDEVVITGQ
                     YKPQSVKNSVYQVRVIGKERILKQGATRLQDVLKNELNMRFSQDVATGGSAISMLGLS
                     GQNVKILIDGLPVTGRQGVNNEFDISQIDINSIERIEIVEGPMSVIYGADALAGVINI
                     ITRKAAAYNLSVNARIHEETVGNEFGIHQGIHNQQAGLTWKKNKWEIGGSFAHNYFGG
                     WKDTAIERELVWHKKDQLIMNGFIGYTNGKLNIRYRVDGLDEVITNPGNFSPFADPIS
                     GDTLAFDQEYLSQRLMHQLQGSYFVNSSLSFQLQSSFTDYSRQVFSTTINKKNGDIRL
                     DPGEGRQSVVDFTGFTFRGTALYKLSDVFSFQPGVDINLDKGLGERLKSGSNAVNDYA
                     LFLTTEITPSSKINIRPGLRFIRNSVYDAPPVIPSINTKFQLTQSLDLRLSYARGFRS
                     PSLRELYFSFFDANHQVVGNPDLKAETSHSFTGSLSFKKKRVEELQYSVQLGGYYNDV
                     KNMIDYVFTQSSDTARLYNVFSSKTSGANLSTVVTYKKWSFSAGAAYTGFYNEQYETN
                     KDLPALVWSAEANASVGYSIPAIGLDLNLFYKLTGKRPRYVMDGPDVVLLEQDGFHLA
                     DFTLNKKLTRSLTLNAGIRNLFDVDRINSSFVQGGIHTSGGLNVATGRSYFASLLFNW
                     NKK"
     gene            16130..17530
                     /locus_tag="PLO70_03450"
     CDS             16130..17530
                     /locus_tag="PLO70_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HQZ73540.1"
                     /translation="MKQKFISISVAFLSLLLLVAGCRKRDADLADNLVVFETSAQGIT
                     ASENSITVKLKLVRGTDRDIPVTINLVEEGVVYGTDYTTTPAAAAGKLTVTIPSGNNE
                     TSFTLTKNSGVLFDGDEKIVFDLFSSGTPVFIGVARQFTLSFAELVAANSTLTINGGG
                     VLYSNKVFIDLSANRQTAVNRNTWDLGFYTGADDYRVILNSSSAMMAKQINKNDLTQV
                     TAADTVGFSTEVAFNQFAPATISLPYIDYPNGDLARTAIMATSAAAAENKVYIVNRGL
                     APGSPAPARGWKKIRILRNASGGYTLQHADIAATTFSTIDIAKEEAYFFKYISFDNGA
                     VSVEPARKKWDIAWSYFSNVTNFGAGEVPYLFQDIILLNRNVQVAKVMAGTRTFADFG
                     IGDIASQTFLTTQNAIAADWRSGGGPGTSPAVRTDRYYIIKDGDNNYYKLRFTALTQN
                     GERGYPAFEAVWLKRD"
     gene            17641..20973
                     /gene="ileS"
                     /locus_tag="PLO70_03455"
     CDS             17641..20973
                     /gene="ileS"
                     /locus_tag="PLO70_03455"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQZ73541.1"
                     /translation="MSAKYREFTGLNLPAFEQEVLAKWTESQAFEKSVSLREGATPFV
                     FYEGPPSANGMPGIHHVISRTLKDLVCRYKTMKGFQVKRKGGWDTHGLPIELGVEKEL
                     GITKEDIGKKISVEEYNQKCREAVLRYKDKWDEITRKMGYWVDLNDPYITFKNEYIET
                     LWWLLSELYKKGLLYESVSIQPYSPAAGTGLSSHELNQPGTYKDVKDTSAVAMFRAVQ
                     DEKSKFLHDVVHGAEVFFLAWTTTPWTLPSNLGLTVGANIDYVLVKTFNPYTHLPINV
                     VLAKALLGKYFKEKGENGDFAGYNEKVKILPWKILTEFKGSQIEECRYEQLLPYEANT
                     AAHAGGDPFRVLVDTFVTTEDGTGIVHTAPAFGADDYKVGKKYNIGILTMVDREGKFV
                     DGLGEFSNRYVKNYKDDPNYVDVNIDIAVKLKKENRAFKVEKYEHSYPHCWRTDKPVL
                     YYPLDAWFIKTTAIKERMVELNKTINWKPKSTGEGRFGNWLENMVDWNLSRSRYWGTP
                     LPIWRTEDGEEEICIGSIEELNTGIKKANEILGVAANKNYLHEGILDLHKPYVDDIVL
                     VSPSGKPMKRVPDLIDVWFDSGAMPYAQWHYPFENKEDFKKSFPADFIAEGVDQTRGW
                     FYTLHAIACLLFDSVAYKTVVSNGLVLDKNGNKMSKRLGNVVNPFDTIEKYGADATRW
                     YLITNASPWDNLKFDSEGIKEVQRKFFGTLYNTYQFFALYSNVDGFAFKEEYIPLNQR
                     PEIDRWILSSLNTVVKKVNDYMDDYEPTQAGRVIEDFVDEHLSNWYVRLCRRRFWKGE
                     YEQDKICAYQTLYECLETITRLIAPISPFFSDAVFQNLNAVTNRFKAESVHHVNFPVA
                     DVSVIDEYLEERMQLAQDASSLVLSLRKKVNIKVRQPLQKVLIPVLNSSMKEQLQKVE
                     DLLKAEVNVKEIEYLNPDNTFISKKIKPNFVALGKKLGPKMKAVSTALSQFAQEDIAR
                     LEKEGQYNLSIDGEPVILQITDVEISSEDIPGWTVANKGNLTVALDVTVTPELEAEGN
                     AREFVNRIQKIRKDSGFELMDRVEIKVAAPDGLKDSLAHFKTYICAEILADKLEFLTE
                     IEDGIEIDINDDLLNVIVSKKG"
     gene            20978..21820
                     /locus_tag="PLO70_03460"
     CDS             20978..21820
                     /locus_tag="PLO70_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQZ73542.1"
                     /translation="MAIKKKKATKKAAKPVKPAKKAVVKKPAAKKTVKKSAPKPAPKK
                     VAKAAPKKPVKPVAKKPAPKPAPKAATKPVAKPAPAPAPKQEPPAPKPVVKKHGGIDP
                     KSLVSIKPKVQPVIKKEPPKPSKMVIPKTSTKSSVKYEPEFTKSVLDTAMQQYSGPTM
                     RYSDADLTEFRGIIQRKLDAAKKELAYLQGLITRKEEGGDMDEGRYMTMEDGSVSMER
                     EQLSQMASRQITFIDHLEKAMMRIENKTYGICRVTGKLIDKARLRAVPHATLSIEAKQ
                     MMNK"
     gene            complement(21888..22622)
                     /locus_tag="PLO70_03465"
     CDS             complement(21888..22622)
                     /locus_tag="PLO70_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73543.1"
                     /translation="MRFFAAVFLLSICTHAQFKKGDRMAGSSVGSVLFNSGSSDITVV
                     SIGSNTSKITSYDVRIIPSLGWFVSDNTVVGINLNINPNGQKTSYEQNGSTYQSDKNN
                     GFNIGIGGFARNYFPGNGSLLPFGQVSLNGGISSLKTEGFFYGGSGSSAYKQTYDGNS
                     SGGFFANAAFTGGFTKLVGENAGLDFYIGYNYSYNKNTFKRTTLRDDGIDGSIDTRAE
                     NETTTKFTNHGFTIGAGFQIFLRGKK"
     gene            22812..23471
                     /locus_tag="PLO70_03470"
     CDS             22812..23471
                     /locus_tag="PLO70_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73544.1"
                     /translation="MRKFRLLSLLFLAITFIAVSCTKEGPEGPAGATGPQGPTGANGA
                     NGPAGPTGPTGPQGPTGPVGPQGPAGPAGTANVIYSSWFPILGTDWTGSGTANQSYTL
                     TAPGVTQAIRDNGVVLVYVTYNNLIRPLPFNDVSTANPITFDFYLPATVGQILLRAYR
                     PSSAFTLITINVRYVIIPGGVAGGRTIGKAAEINGQVYTESQLKAMSYQQVCSLLKIQ
                     P"
     gene            23571..24914
                     /locus_tag="PLO70_03475"
     CDS             23571..24914
                     /locus_tag="PLO70_03475"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQZ73545.1"
                     /translation="MKNIALLLFCLLLTIITIAQPRIISGPMLGPVELRDAKVWLEVS
                     PEVKSVSLQYNKKGEKKTKTVLYKGELGNEFNPLYFTIGGLEFNNTYQYKIWVNGKAT
                     TYGGEFTTKDLWQWRKPIPEFSFLTGSCSYFNEPVFDRPGTPYGKDSSIFETMSKEKA
                     SFMLWLGDAWYTREVDYFSEWGLWYRASRDRATPVLQNFLKSMPQLATWDDHDYGPND
                     IGKNYILKEASRNVFNKYFCNPSSGENGQGIYTMTTWGDADIFLTDDRWWRSADRMKD
                     SVDGKPNPEKRMLGKQQMEWLKNSLLYSSATFKIITVGSQVLNPVSPYDKWRDFPAEY
                     QELMDFLKEYKVNGVLFLTGDRHHSEIIKVDRPGTYPLYDITVSPLTSGTHSFGGPEK
                     NNAYRVFGLDEKQNYGKFSFSGTRGNRKLTVEFLGVKGEKLGEWSITEKELKTPQ"
     gene            24924..25403
                     /locus_tag="PLO70_03480"
     CDS             24924..25403
                     /locus_tag="PLO70_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HQZ73546.1"
                     /translation="MTIYFGYEKGQVIQALRYHFISRQEIRIMLILVNVFALASVTLY
                     ALGKITPMAFFMGSALWIVLMISFWFILPFMVYRRAITFKHEFSMDFRDDGFTLAHER
                     GSKSWAWTALKSYLESPHFFHLYFDSRSFLLVPKNGCKDSDEVFELRKLIKEKVKKG"
     gene            complement(25431..25859)
                     /locus_tag="PLO70_03485"
     CDS             complement(25431..25859)
                     /locus_tag="PLO70_03485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ73547.1"
                     /translation="MALKIIEHGTPDFQKMVKLREDILRKPLGLGFTTNELEQEKSNI
                     LIGAFEDEDILGCCMLVEENPEIVRLRQMAVLNDLQGKGIGRAIMHFAENIARDRGYS
                     ILSMHARKNAIGFYEKMGYKVTGDEFTEVTIPHYVMEKKL"
     gene            26074..26310
                     /locus_tag="PLO70_03490"
     CDS             26074..26310
                     /locus_tag="PLO70_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQZ73548.1"
                     /translation="MSDIASRVKKIIVDKLGVDEAEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAETITTVGQAVTYLEEHAK"
     gene            26459..27712
                     /gene="fabF"
                     /locus_tag="PLO70_03495"
     CDS             26459..27712
                     /gene="fabF"
                     /locus_tag="PLO70_03495"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQZ73549.1"
                     /translation="MELKRVVITGMGAITPLGNTVQDYWNGLINGVSGADMITLFDAS
                     KFRTKFACEIKGFEPTQFLDKKEARKIDRFTQLALVVSDQAMTDAGLNKENINPDRVG
                     VVFASGIGGLITFQHEVTDFARGDGTPRFNPFFIPKMILDIAAGQISMRHNLRGPNFA
                     VVSACASSTNAIMDAFNLIRLGKSDIILTGGSEAVISEAGVGGFNAMKAMSERNDDPK
                     TASRPYDKDRDGFVMGEASGILVLEELEHAKARGARIYCEVAGCGATADAYHITAPHP
                     EGLGARNVMLAALDEAGMKPDEIDYINTHGTSTPIGDGAEVKAITAVFGEHAYNLNIS
                     ATKSMTGHCLGAAGVIEAIASIQAVINDIVPPTINHFTDDPELDPKLNFTFNKAQQRT
                     VRAALSNTFGFGGHNACVIVKKYKA"
     gene            27741..28499
                     /gene="rnc"
                     /locus_tag="PLO70_03500"
     CDS             27741..28499
                     /gene="rnc"
                     /locus_tag="PLO70_03500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQZ73550.1"
                     /translation="MDFFKNFFTKHEGSSFKKELKNMLGYKPGNISLYKTALTHRSIR
                     DGADENNERLEYLGDAVLSALIADYLFKRYPYKEEGFLTEMRSKMVNRQQLNEIAIRM
                     GLKKVTLYNKMDGSLKVSQIFGNTLEALVGAIYLDLGYKKTAKWVLEVIILPHMFMDD
                     LENLEINHKNKLYGWANKNGRVLEFETLNEKLENGRRLFTVAAVLDGKNIAEGKAFNK
                     KDASQIAAQVAVEKLGIVNSDSVGEEPDEKKAQA"
     gene            28523..29074
                     /locus_tag="PLO70_03505"
     CDS             28523..29074
                     /locus_tag="PLO70_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73551.1"
                     /translation="MYLLKRIALLFLFIAVFIACNNPVKGRNGVVFKNPADYNDYIIS
                     RQTILIKNILDLGKKADSDVDSAYGMLDSYTNDATRIIAEIKDMPSYKGDSSLRDAAV
                     NSFTFYKKLFGESYKRVLSIRREGGDETEAGAAEIMGIMDSIKREEEIHDKRLHNAQK
                     DFASRFKMKMRDNEIQKEINQNQ"
     gene            complement(29079..29480)
                     /gene="mce"
                     /locus_tag="PLO70_03510"
     CDS             complement(29079..29480)
                     /gene="mce"
                     /locus_tag="PLO70_03510"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQZ73552.1"
                     /translation="MQKVEHIGIAVKNLANSIPQFEKLLNAPCYKTEMVETEKVNTAF
                     FRQGETKIELLESTDPEGVIARFIEKKGEGLHHIAFDVTDIEAEMKRLQGEGFVLLND
                     KPKLGADNKLVCFLHPKHTNGVLVELCMEKK"
     gene            29610..30827
                     /locus_tag="PLO70_03515"
     CDS             29610..30827
                     /locus_tag="PLO70_03515"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HQZ73553.1"
                     /translation="MLKFPIYLDHNATTPCDPRVVEAMIPYFTNSFGNAASRNHPFGW
                     QAEEAVDYAREQVARLIGADPKEIIFTSGATEGDNLAIKGVFEMYASKGNHIITCNIE
                     HKAVLDTCKHIEKEGGEVTYLKVKPNGLVDLAELEAAIKPTTILIAIMYANNEIGTVM
                     PMKEISAIAKKHGVLVFSDAVQAVGKIPVDVNKDGIDLMAFTAHKMYGPKGVGALYVR
                     RKNPRVKVTAQIDGGGHERGMRSGTLNVPGIVGFGKACEICINEMEADTERIIKLRDK
                     LENALLQVEESYLNGDKDYRLPHVSNISFKYVEGEGLMMGFNKNIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSLRFGLGRYTTEEQIDYTIEQVTNTVNKLREMSPLWEMYK
                     EGIDMNSIEWAHH"
     gene            30852..31271
                     /gene="iscU"
                     /locus_tag="PLO70_03520"
     CDS             30852..31271
                     /gene="iscU"
                     /locus_tag="PLO70_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HQZ73554.1"
                     /translation="MAYSEKVIDHYQNPKNVGTLDKSKANVGTGLVGAPECGDVMRLQ
                     IEVDETTGLIKDAKFKTFGCGSAIASSSLATEWLKGKSIDEAVTIDNMTIVEELNLPP
                     VKIHCSVLAEDAIKAAINDYRKKNGLEELKFDESVIH"
     gene            31359..31712
                     /locus_tag="PLO70_03525"
     CDS             31359..31712
                     /locus_tag="PLO70_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQZ73555.1"
                     /translation="MSVGLDNAIYVSDKAKAKVQQLLADAGVANDPSYFLRVSVVGGG
                     CSGLSYKLDFDNEQKPMDQAFEDNGVKVVTDLKSFLYLVNTTLDFSDGLNGKGFYFSN
                     PNASRTCGCGESFAV"
     gene            31830..32555
                     /gene="gldF"
                     /locus_tag="PLO70_03530"
     CDS             31830..32555
                     /gene="gldF"
                     /locus_tag="PLO70_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HQZ73556.1"
                     /translation="MPMWSVCKKELRQFFSSLTGYIAIIVFLLVNGLVLFVFEDNILD
                     FGYATLDRFFQLAPWILLLLIPAITMRSFAEEFKTGTYEILQTRPLTRWQIIGGKYLG
                     SLIVVFIALLPTIIYIFSIQRLSSNEGIDMGATIGSYIGLFFLAAVFTAISICCSSFT
                     NNAVVAFIVSLIGCALLYYGFSAISRMPALANGPDYYVEMIGIDFHYRSISRGLIDTR
                     DVVYFLSFIFLFFAITNRNLLKR"
     gene            32557..34308
                     /gene="gldG"
                     /locus_tag="PLO70_03535"
     CDS             32557..34308
                     /gene="gldG"
                     /locus_tag="PLO70_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HQZ73557.1"
                     /translation="MRKKVEKIFASKYWWLMLLVVLLIVNYLGSVFHARLDLTKEKRY
                     TLSKATMDILKNLDDDVQINVFLKGDFPAGFKKLANSTDEFLQLLKDRNGSKIHYRFV
                     SPVEEIPGTKIIYGDSLVNLGAIPINLTVQKKEGESSNIIFPVAIIEYKGKQSLVNLY
                     PGANSRISQEEINGAESLMEYQFVKTLDKLTASKKPGIAYSVGNGEPTDGRTFMFRQA
                     LADDYDFKIINLHNQPRIPDGVDVLMIVKPTIQFTEDEKLKIDQFVMGGGKLLCFIDN
                     LIAEQDSLAFKPETIAYDRNLNLTDLFFKYGLRINTDLVMDLQCDLLPFVVGGTNENP
                     QMELLSWNYYPMLNPTNSKLTKGLGYVGSHFANSIDTIQVNGVRKEPLLVTSPNSRII
                     STPALISLNENKNVPEDVNFKRNEIPVAMLLEGRFTSLFKNRISRAQLDTLSLIGEAF
                     RETSEVSKIIVAADGDILFNDFLPGEGPNAAPVPIQMGWNKYTYREYLNQTETGRFFR
                     PVANREFLLNCIEYQVSNPGISETRNKDVVLRLLDSQKVKEQKTTWQFINIALPVLLV
                     ILFGWIYQQVRKRKYAA"
     gene            34346..35314
                     /locus_tag="PLO70_03540"
     CDS             34346..35314
                     /locus_tag="PLO70_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HQZ73558.1"
                     /translation="MTADQITYLVFGIVLVFALVFDLGLLSKKGQKVTIRQALYQTFF
                     WVGLALAFFVFVWIEKGQGLALEYLSAYLMEWSLSIDNIFVFILIFSAFSVKEKHFGR
                     VLLIGILMAIIFRVIFITVGVALVERFHWILYVFGAFLVYTGYKMFTATDDEEFDPQG
                     SKIFLFIKKFLPLAPHDGNGKFVVKENGKSVYTTLFVVVIMLAAIDLVFALDSIPAVM
                     GISRDRLVIYTSNIFAVLGLRSLFFLLRGAVTKFDYLQQGIAIVLVFIGVKMLTEHWI
                     NQWVSKQIQVFISLGVIMICITGSIFYSIFINKRGVPPEVKDDSVS"
     gene            35338..37737
                     /gene="alr"
                     /locus_tag="PLO70_03545"
     CDS             35338..37737
                     /gene="alr"
                     /locus_tag="PLO70_03545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQZ73559.1"
                     /translation="MNYSVSNIAQIIHADATIVKDLSAEHLLLDSRKVFSPGSSIFFA
                     LVGFRRNGHDFIPDLYKKGVRSFVVSEKFETAAYPGANFLLVNDTLAALQQLAAHHRK
                     QFAIPVIGITGSNGKTIVKEWLYQLLHTDYNIVRSPKSYNSQIGVPLSVWQMNEHHTL
                     GIFEAGISKPGEMDKLERIIQPTIGVFTNIGEAHSEGFESMKQKEEEKKILFRNATET
                     GKLDVVEVRYQQSTTIITAKDPRAENEFISIEIPFTDDASVQNATTCWKVMLTLGYDN
                     EIVRFRMKQLLPVDMRLEMKKGINRCLVINDSYSADLSSLEIALNFLVQQSAGTQRTV
                     ILSDFLQSAVADEKLYAQVVENLHKHKVDRLIGIGGRISNSLRHLINRADKRFAVELY
                     PSTDIFLQQYRSSMFKEEAILVKGARIFAFEKIVQLLEQKAHQTLLEINLNAIVHNLK
                     EYQKVLKPTTKIMAMVKAFAYGSGGAEIAGILQYHKVDYLGVAYADEGVELRNAGISL
                     PIMVMNPEENAFDVIVDHNLEPELYSFELLNSFDSFLQKEGIQHYPVHIEIETGMNRL
                     GFSIAEIEKLCTSLNSTSSFKVQTVFSHLVASEDALQDPFTINQFNKFGIATTLLQER
                     LGYSFIRHIANSAAAIRHQQLQLDMVRLGIGLYGVDSAGSGKLNLQTVTTLKSTIAQV
                     KHLEAGESVSYNRNGVVNRDSVIATVRLGYADGYPRRLGNGVGKVWVKGHLVPVIGTV
                     CMDMFMIDVTGIDNIQEGEEVVIFGTNLPVQQVAKWAETIAYEILTGISQRVKRVYYE
                     E"
     gene            37857..38558
                     /locus_tag="PLO70_03550"
     CDS             37857..38558
                     /locus_tag="PLO70_03550"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HQZ73560.1"
                     /translation="MKLRKIIFLIAVIIIIDQALKIWIKTGQPTGEVVRVFGFDWFRL
                     HFIENPGMAWGWKFGNETGKMILTLFRLAAVIFGTWYIGRIVKQQYSKGFIVCAGLIY
                     AGALGNLIDSMFYGMIFDKGLHYDAAINDYISYGGIAQFSSNGYSSFLHGSVVDMLYF
                     PIIKSNFPSWFPVIGGDEFEFFSPIFNIADASISVGVITLLIFQKRFFKKHHETVTSP
                     TVETNTEISDKMQVL"
     gene            complement(38571..40886)
                     /locus_tag="PLO70_03555"
     CDS             complement(38571..40886)
                     /locus_tag="PLO70_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ73561.1"
                     /translation="MKKNLRRTIFSPLFFLLPVLLISGITSAYSQNIDFGKSYINVTK
                     GLNGGTVETGDTLEIRASFVVRSGTYDSCRYQDAVPAGTTYIPGTIKVLTNEGIPYKS
                     FTDAAGDDQGRITGTAITINLGFRTGAAPATAFRRGRVANTHKPSFYGSTCIMIASFR
                     IRVTAATGSNISTGGGNITYKNGAAAIQTFTFPANTLRVYPNYGICSNTIGTNAIGTE
                     FNGTFGSGQPRNRGTSANVPVGYTFNSFGINSPQDYYYGVANNTSTNTGYTTLNTWPK
                     PDPDPDGAGPLTSHRVFTLWDIIGDHTGAVNPIAGNPAADTVANPNAGYMLVINAAYR
                     IDSAFQQTITSLCPNTYYEISCWMRNICSKCGCDSNGKGATNAAGPPFYIPTGPGDSS
                     GVAPNLTFEIDGIDYYTSGNLLYSGQWVKKGFTFLTGPAQTSFTLKFFNNAPGGGGND
                     WALDDISVSTCSPNMKYSPSLSPAVCDSNVITIYDTVRSYFNNYTYYKWQRSTDGGAT
                     WSDVTAPAGPASPFWNGTAWEYVISYTLTPAQTQMPNNGDMYRVVVATTLSNLTDPNC
                     NFTDQGNIITLRVIFCGIPLKTRLLSFSGRQENEHAILKWTTTGENEPIIFDIERSTD
                     GINFIFAGSVNSYNDYISEQSSYSYSDPEKLTGKVYYRIRMRNNTGQSTYSRTIQLAS
                     SADILSFVSVINPFNNELLFEVSSQNSGKAEAELFDASGKSVRKKAFDISSGITRLSL
                     ENTSKLTTGIYFLRVQSAGKFIQKSVLKTSN"
     gene            41065..42744
                     /locus_tag="PLO70_03560"
     CDS             41065..42744
                     /locus_tag="PLO70_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQZ73562.1"
                     /translation="MKKPLCVIFRILVLLFGFFTAAAQNEPFTKRTVVSGLNSAWEVV
                     YGPNDSLWVTENRAYLISRISIATGAKTVLVDLRATDGSINFTSGTGTQPQGGLMGLV
                     IHPNLYSADPAVRAAKPWVYAAYVYNRGTCPGTNTSCIFTTKIVRFDYNGNTLSNPVT
                     IINTIPGSSDHNSGRLVISPVIEPGADAAHTQYRLYYTVGDMGAGQFLNTTRAQNAQN
                     VNIMEGKVLRINSESDGDAGQDAWIPNDNPFYNGAVITPQDYVFTLGHRNAQGLVWGT
                     VGGNNILYSTEQMDRTDDEINIIEAGKNYGWDQVSGYCDNNVNGYKIGQNTSANEGAF
                     CSVTPTHQEPIFTTFTETAANMPALMAQSNNQLWPTIASSSVDFYGQNKIPGWNYSLL
                     ISPLKKDRIYRVSLNATGTDVIGDTIPYFRNDGNRIRRVTISSDGLKFYVARDAGATI
                     NGGAIMEYTYAGVILSLNENPGVRPVRVEDLISIYPNPATRTIYIHGKRELRKPLLVQ
                     IYDVHGRMLKEETSFRNDFSVDISRLIAGTYILKLYNGNDMEVQIEKFIKQ"
     gene            complement(42855..43079)
                     /locus_tag="PLO70_03565"
     CDS             complement(42855..43079)
                     /locus_tag="PLO70_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73563.1"
                     /translation="MKSNARLISSFPLNTLKPVKPEPQSSSQQIKSAYLKAILSGSRI
                     ISGTESAVPVENPDRAAAIRYIKESASTRK"
     gene            complement(43218..44618)
                     /gene="fumC"
                     /locus_tag="PLO70_03570"
     CDS             complement(43218..44618)
                     /gene="fumC"
                     /locus_tag="PLO70_03570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQZ73564.1"
                     /translation="MEFRIEKDTMGEVKVPADAYYGAQTQRSIDNFKIAQDINRMPKE
                     IIRAFAYLKKAAALTNLEAGVLAKNKAVLIGKVCDEILKGKLDDYFPLVVWQTGSGTQ
                     SNMNVNEVVAYRGHVLKGGKLTDKEKFLHPNDDVNKSQSSNDTFPTAMHIAAYKILVE
                     TTIPGIKKLRDTLAKKSKQFMKVVKIGRTHFMDATPLTVGQEFSGYVSQLDHGLKAIN
                     NSLAHLSELALGGTAVGTGINTPAGYDKNVAKHIAALTGLPFVTAENKFEALAAHDAI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGELFIPDNEPGSSIMPGKVNPTQCEA
                     LTMIAAQVMGNDVAISIGGANGHFELNVYKPVMIYNFLHSARLIGDGCVSFNDKCAVG
                     IEPIEANIKKHVDNSLMLVTSLNTKIGYYKAAEIAQKAHKQGTTLKEMAVKLGYVTPE
                     QFDEWVVPANMVGKLK"
     gene            44750..46177
                     /locus_tag="PLO70_03575"
     CDS             44750..46177
                     /locus_tag="PLO70_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQZ73565.1"
                     /translation="MNRIVLFFIFLFIAGSPMAQQNAPSPLPTKQQLAWHENEYYLFV
                     HFGPNTFTDKEWGHGDEKEEIFNPTALDCRQWCRVAKAAGAKGIIITAKHHDGFCLWP
                     SKYSTHTVRESKWKNGRGDVLKELSQACKEAGLKYGIYISPWDRNHPDYGTEKYNDVF
                     VGMMKELFQNYGPIWELWWDGANGEGPNGKKQEYDWRRYENTVRKLSPSTVIFSDIGP
                     DIRWVGNEKGIAGDPNWNFLDTTGFKRGIGAPDTDTLNQGNYYGKHWIPAECDVSIRP
                     GWFYHANEDQKVKSPEQLFDLYLKSVGRGANLLLNVPPDRRGLIHEKDSAALIGFKRL
                     RDESFSDNLLKKADTYYEFSHRDFIKKPLLLRGFDSAAAYYGINLQNFIADFRRPVTV
                     NCIVLREAIHLGQTIRRFNIELYNNDRLVGKVQGHSVGRKRILTFPAITITSFRVNLE
                     DAKGNDNISGVAAYLINENLIENEK"
     gene            46167..47384
                     /locus_tag="PLO70_03580"
     CDS             46167..47384
                     /locus_tag="PLO70_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQZ73566.1"
                     /translation="MKNKPGLRIKIVLTSFLLCFSWILLAQKQDRYMEKLIRENFMLA
                     AQQYKLLAGNTPTNVMPRNYMAKEGKLVTSNTTWWTSGFFPGSLWYIYEFTKDSAIRA
                     EAERRLAIIEHVKHYTGDHDLGFMIFCSFGNAYRITGNQTYKNVIAEAAETLIKRYRS
                     TTRSIQSWGRMKDSISPVIIDNMMNLELLNWVSDEWREPKYKVIAIDHANTTLKNHFR
                     PDNSSYHVLDYDVNTGNVIRKKTAQGFSDESAWARGQSWGLYGYTMMYRFTKESSYLE
                     QARKIALFLLNHPNLPPDKIPYWDYNAKDIPDTYRDVSAGSIMASALLELAQYEVDAK
                     KKKEYIAVAKKILQSLSSDSYRNKPGESGGFILKHSVGSLPGNSEVDVPLTYADYYFL
                     EALLRYKNWYLKK"
     gene            complement(47387..48166)
                     /locus_tag="PLO70_03585"
     CDS             complement(47387..48166)
                     /locus_tag="PLO70_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQZ73567.1"
                     /translation="MNAQTLLAILDNGILTIMINRPEKLNALNKDVFSDLNKALDEIE
                     NNPEIKSVIITGAGSKAFVAGADISEFGGLNREEAMALAKRGQDTFARIENSSKPVVA
                     AVNGFALGGGCELAMSCHFRVAAENAKFGQPEVNLGLIPGYGGTQRLVQLIGKGKAME
                     LLMSAHMIDANEAKQLGLVNYVTTPETLLEHTRKILEIINSKASLAVAGCIKSANAVF
                     NETKDGYALEIEEFGNAFATEDMKEGIAAFMEKRKASFTGK"
BASE COUNT        14641 a         8935 c        10566 g        14201 t
ORIGIN      
        1 tttaagttca gcttctgaat aagcgccata agttttactc caccccggca tatccatgtg
       61 tattggtacg tttggtcttt tggtgtcatc catagtatgg tcaatcttat tagcatatat
      121 tttgacctgc catttgtata gtgactttga tggattttcc cggtgcatac tcaatgagcc
      181 aatcctggct gctgcatatc ccacatccat aggcaaggct gggtaaccaa tattccatcc
      241 atcatcgccc agcaggtctg ctttaaaata ggtgtatgtg ttgtgcttat attttacgga
      301 taaagagtag ttcgcttttt cgaaatgtga aaaatcaatt acatcaccac cactacttcg
      361 ataattttta ttgttgcggt aagtaccact catcctgaaa gcccatttat ccgttgaata
      421 atttaaccgg gcagattcgt aaaaactttt ggcagccgtt tgataaccac tgcttatcat
      481 ccctgttatt ttatttgcat tcagataatc cggttctgcc atcttcatat ttatagtgcc
      541 accaacagac gaaccgctca taaatccggt agtaacagtt tgtacctgca ggttttccag
      601 gttaatgggt tcaatataaa ttgtagcggg gtccatttta tcagtacagg ctccgtgaat
      661 cctcatgcca tctactaata cattgatctg tccaccatta aatgaacgta gagtcggttc
      721 catgccgtat gaaccccggc gtaacaggga aacctccggt aagcggctta tgatttcttc
      781 aagagtggag gaattatttg atctgaaaaa gttaaccagt agtcgttggt gatttttttg
      841 atttgtgctt atagttatct ccctcagttc aatgatgcga ctgctgtcat cattcattgt
      901 tgattggtat agttgggcgt ttatcaacaa acagctgttg acgagtagta aactaataat
      961 taatattttc aaggttgtaa aatttaaagc aggtgggaaa acccacccgc tgttattaac
     1021 tgctaaaatt caacatcaaa atattgtgtg gtatcagcta cagctgcacc tgacataaaa
     1081 tcaaggttta gtctccagag acctgtcata gtgaagttta cttttccttt gtaatgacca
     1141 ttgcctatat gggtcgggtt tacattatta ggtgagccat ggcccatcgt gggcatttcg
     1201 ggtgttagta taactgagag actgctgtca gccggccagc tcatcataga tacttttttg
     1261 taaatagcta tttccatatc attgataccg acttttgaac tggcagcagt aggttcaatc
     1321 aaggagataa agtatttggc gccattatgt acagccgtga atgatttttg tctggacctg
     1381 gtgggttcat ttacagtaat gggaatagtt aatgatcctt ctttatcagt aaggttattt
     1441 tgcacctgta ttttaagtgt ccaggttcca cccattgatg acattataaa ggttacactg
     1501 caagggaata aatggttaat agttttttca gaagccggat tttcataagg cgaggaatgt
     1561 atcattgttc ccatatccat aataggagta agttgtatat gggcatcttc aatcctgttt
     1621 ccagatacag aatcatacag cgcaatatag aattttgtgt agccagcgtt tattgaagct
     1681 tcttttgtgt acacttctac tttagttgcc gccccagcag catacccttc aataattttt
     1741 gttaatcccg cgactggatc tggctcctgc acatcatctt tcgaacaggc agaaaggaat
     1801 acagcaacac atagaactgc aagtgatagt aatatcagta tagatttttt cataaaaaca
     1861 gtatttgttt gaataattgt atgaaataca ggtacagcga tgctttttta acgcatcaat
     1921 taaaaacggg tggcaattag atcggtatga tcaactgagt gaagggggac gaaatacagc
     1981 aatagtatga ttgaagctat aagtagctgt actgaaggga ttataaataa gatcgatttc
     2041 aggagcagaa atcgaatagt tgattgtagt tatagtacaa ggactagttt caagtaattg
     2101 tttgatctgt aagggagctt cttcgttctg gtgttgttct gtttctttta ttttgttatt
     2161 gagaaagcac ttgccatcac attttagttc cggacggtct ttgttaatac agtattcttt
     2221 ggctatagtg gttctgttgg caaaaaacca gatggtaaaa ccagtagcat agaaaagctg
     2281 gaatgaaaaa ataaaaacaa gtatgatcgc aatgactttt ttcacaattg caaatatagc
     2341 cggcgatata aaaaatggca gctgatttta atcagtagcg ggtatgatta aagacatcaa
     2401 tgtcttttat agtcattaat attaatgagg tgtgtacaaa acttatattc agtttcgtca
     2461 ttgctgctta ctgccacaag ccggtgctta cagtatttat tgatcaggga gtgatataag
     2521 tcaatacctg ctgtatcaag gttagtacac ggttcatcga gtaaaacaat catcgtatca
     2581 gaaagtatgc actgggcaag ttttacccgt tgtttcattc cggaggaata ataccggatc
     2641 tgcttatgaa ccgctttttc caggccaatt atagaaataa tttcttttgc agaaagtccc
     2701 gggagccacg gtttaaatcc ctggtgaaaa ttcatgaact cagtcaatgt catctcttcg
     2761 atcacttcga ggtaaggagc gcagattgat acatgctgat atatttgttc attggctaat
     2821 tcattgccgg ttattgccca ttgcattttc ccttcattga tcatcatacc accactgatt
     2881 acttgcaaca atgtactttt tcccgaacca tttggtccgg tgatggcata tgcatttcct
     2941 gtttcaaatg tataagtgaa atgacggaat atccagtcac ggttaaaccg ctttccggca
     3001 tcagataggg agatcgtcat cgttgctgcc tttagtgaac cgtttgatga tgccccttcc
     3061 actttcacgg ataaaggtta ctatttcatc ccgttcatca gtagcgggca tttcttcttc
     3121 aatatattca acagcctggc tggtgttcat ccctttatta tacagtacac gataaatgtc
     3181 aaggatatga ttaatacgtt ctacactgaa gccgcggcgt ttcaatccga ttgagttgac
     3241 accggcataa cttaaagggg tacggcctgc ttttatatac ggaggaacgt ctttcccaac
     3301 aagtgagccc ccgcttataa atgaatgggc accaatggtt acaaactggt gtacagcaca
     3361 catgccgccg ataatagccc aatcgccgat ggtaacatgc cctgccacct gtgtattatt
     3421 actggtaatg caattatcac ccacaaaaca atcgtgggca aaatgacaat aggccatgat
     3481 aaggcaattt tttccgataa cagttttatc cctgtctttt gtaccgcggt tcactgtaac
     3541 aaactcacgg atggtggtac catcgcctat ataaacgttg gaatattcac cctggaattt
     3601 caggtcctgc ggaatggcag ctataactgc ccctggaaaa atgcggcagt ttttaccgat
     3661 cctggcacct tccatgatcg ttacattaga gccgatccag gttccgtccc caatttcaac
     3721 gtcctgatga ataacggtga aagggtcaat ttttacattg gtggccagcc ttgcattagg
     3781 atgaatatat gtatgcggat gaatcatgca attttaaaaa ttcccgcccg gggcgggacg
     3841 ggcaaagtta gttgttacgg ccaaaacccg aagttttttt ggacatatat tgttaaaaat
     3901 cagcatctga tcagtatcat tcggcgttac accattaatc tgcatgataa agcgtaattt
     3961 cgcagttcgt atgcacaatc ctccggatat actgacactg gcttataaac aaatcctcat
     4021 gcaggaccag atggtgctgc tacaggaaaa agagcagcag acacccggtt cggtacaata
     4081 cactattaac aggtaccgtc gccatccgca atggaatatt gaagataccg gcatgctcgt
     4141 atatcattat gaaaagaaag agccaaaaga aaactacctg gagcttaaat tttgtgtgag
     4201 tggtaatgtg tactgccgta aaaaagattc agaatgtgat atgtgccagt tcaatgcttc
     4261 caaaacctgc atggaaaggg tggaaagtgt ggatgtggta agtttcaggt tttcaccggt
     4321 gcatatgagc caatttgtaa agcccagaaa agggaatgca gcacttactg atgatatttt
     4381 aagtttcagt cataattctt cattcaccaa gattcttcca ttatgtggca aaacaagaat
     4441 ggtgttggaa ggagtcttaa atcataccta cagcgacagc ctggagaata tttatatcaa
     4501 tgcccaaacg cagatgttgc tactctacag tttggattgt atgctgggag aaaaagagat
     4561 tgatgtcatc agctgtaaat tcctggctaa tgaagcagac agggaaaaaa ttgtaaaagc
     4621 aagggaaatc ctcattcagc atattggtga gccaatcacc attaaagaac taagccgtaa
     4681 agttgcaatc aatgaatgct acctgaaaaa agggttcaaa gaattattcg gcacaactgt
     4741 atttgatttc taccaaagcc agcggatgga gcatgccaaa tacctgttgt acgaaaaagg
     4801 attaagtgtt actgaagtct ctatgatgct tgggtactct tctatttccc acttctccac
     4861 cgccttcaaa aagcacaccg gtttaaaacc atgtgaactt ttattacgat aattcattag
     4921 tgagtttact atcatttagt cgtattagac tgtcgtaata tttatctaaa tagataagca
     4981 gggcgcaata ttatttctat gccatccgtt acaggacaca tggcatcatt tttgaatatt
     5041 tagttgtcaa ttgtcacaga tgcgtaaaac gctggccata ctcctgatta gtgtacacct
     5101 tgttggtaat acagaggctg gtcagctatt taaattgcca cagcttcttc agcatttctt
     5161 tcagcatcat catcttgatc cttcaatcaa ttttattgag tttatggcaa tgcattatgg
     5221 tggagatgat ggaacttcag cagatgatga tttcgatcaa gaacttccct ggcataattt
     5281 gaatcaacat tccattgcgg ttgtctattt tccaatgatt aaggaaattg cctcaactga
     5341 gttctcctat ggggaaacca aggtatatgc cagtcattta cctcctgcta tttcagcgaa
     5401 acatgcttta ttaattctgc agccgccccg gcttggatag tattcttctc atttacctca
     5461 attgttaatg taatctgaat tccagattac agggttacat aacctatgta tctgtttact
     5521 gattcttttt taagtgtatc tgtgctgtat ggcactgagt atgatttata aatcaaaaca
     5581 acctttatga aaaacaaaat tgtttatgtt atattctctt taatcgcagg actttttata
     5641 ttgaatgcct gccggcatga aattctgaat cccggaaatg gcagcggcgg tggtggtact
     5701 ggcggtggtg gtaatcttcc acccacatca atctgcaacc cggattctgt ctattttgtt
     5761 aatgatatca tgccgcttat ctcttctaat tgtacgatga gcggatgtca tgataatatt
     5821 actcatgcag atggagtaaa ccttactacc tatactaaaa taatgggtta tgtaagaccc
     5881 ggagatgcgg ctaacagtaa gctgtataag gtaatcatta agacggatgg cgatagaatg
     5941 ccacctcctc cgatggcacc aatgacaaca gcccaaaaag cacttatcca gaaatggatc
     6001 aaccagggtg caaagaataa tagttgtata ggcagttgcg acacaactgt atttacctat
     6061 agcggtgcag taaaacctat aatggataat aaatgtgctg gttgtcacaa cccgtcaaat
     6121 cttggaggaa atattgatgt gtcaactttt gccgcagtca gggtagttgc tttaaatggt
     6181 aagctatatg gttctatcgc tcaccagacc gggtattccc cgatgcctaa aaatgctgct
     6241 aaactatcag attgtgaaat ccgccaggta cagaaatgga ttattgctgg ttcacaaaac
     6301 aattaaatca aaaagaatga atataaaatt ttcaatactg ggtatactga ttgtatcagt
     6361 actttttgtg agctcatgtt actatgataa agaggattta ctgtatggtg aggctaatag
     6421 tgccccttgt actgatacaa caggagttat atcttataca caaaaaatag tgccaatgct
     6481 gcaacaatat tgctacagtt gccatacagg aagctttcca tccggcaatc aggtgatggg
     6541 cacttatgct actgataaag ctatggcgca aaacgggaag ttatatggaa ctataaacca
     6601 tgctgccggg tattcaccta tgcccaaagg catggccaaa atgagtaatt gccagatcgc
     6661 taccataaaa aaatgggtag actccggtat gctgaataac taaaaaaagc cactatttat
     6721 gaaacgaaaa aaaatcattc tttatgtgtt gctgtcagta ctggtgatcg gtggtgcagt
     6781 tggtatttat atttataaag agtataaccg ggcacataag gatacggctg gcctaaaacc
     6841 tgatttcaca gttgccgcta atgacctgtt gaaagaattt gaaaataacg aacaggcttc
     6901 aaacaaaaaa tactgggata aggtgctgag cgtagaaggt atggtaaaag atgttacaag
     6961 ggatgacagg ggtttttatt ctgtcatcct tggagataca tcctctttgt cttctgtccg
     7021 ctgcagtatt gatagtattc atggcaatga agctgcttcc gttaaaaggg gacatatagt
     7081 ctccgttaaa gggatatgtt caggttacaa tgcagatgat atgcttggtt ctgatatcat
     7141 tcttgtgcgc agcgttatcg gacaaaaaaa ataattcact aacacctaaa aattttgaac
     7201 atgaaaaatc acttgttact ggctacggta ttaatgttgg tcgctacttc ttcgtttgga
     7261 caaaaattct ttacaaaaag cggaaagata gattttgatg caacctcacc tgcttctcct
     7321 gaaaaaattg aaggggtgaa cagaacggct acctgtgtgg tagacacgaa aacaggagct
     7381 atacagtttg cggttttgat gaagggattt gcatttgaaa gagccctgat ggaagagcat
     7441 ttcaatgaaa actatgtaga aagtcataaa tttcccaagg ctgagtttaa aggaagcatt
     7501 attaataatg aaacaattaa ttacgcaaaa gacggcacct attcagtaac agtaaaagga
     7561 aagcttacca tacacggtga aactaaagaa gtggaaacac cgggtaagct tacagtacag
     7621 ggaggtaaga taaatgcaaa tgcttcattt tctatattac tgtcagatta taaagtatca
     7681 ataccaggcc ttgtagcaga taaagtttcc aaaacggcta aactaattat ctcctgttcg
     7741 cttgaaccac ttaaaagcta aaatccaata aaactattta tgaagaagct atcatctcaa
     7801 tttgtcacag gcgtagttct tttactgttc agcagcaatt tatttgccca ggacgaggac
     7861 cttctgaaat tagtgggcgg agacgagaag ccaacgaaag aaagagtgaa atatgcgttt
     7921 aaatctccga gagtcattaa cggacattca atggaattcc tgaatccggg aacgatggat
     7981 ttcagaatac ttcaccgctt cggtcaactg ggccaggggt ataaaaactt ctttggtctg
     8041 gaccaggcca gtatgcggat ggggtttgat ttcggtatcc tccaaaacct gatggttggt
     8101 gtaggaagaa gtacctataa gaaagaagtg gatgcattta ttaagtatgc tccggttcgg
     8161 caatctacag gaccatggag ttcacccgtt acatttgcgt tagtatcagg tattacaatg
     8221 gatggattac catgggctga ccctaaccgc aaaaattttt ttacttcccg tcttgcttac
     8281 tatttccagg caataatcgg gaggaaattc agtgaaggct taacgctgca ggtttcgccg
     8341 actatggtgc ataagaacct tgtacaattg caaacagatc ccaataacat ttatgcgatt
     8401 ggtgtcgggg gcaggcttaa gttgtctcag cgtattgcat ttacctggga ttattctcat
     8461 gtaataatag gcatgccgga tagcggctac tatcatcctc tatctgtagg tttcgacatt
     8521 gaaaccggtg gccacgtctt tcagttgcat ttttccaatt caacgggtat gaacgaaagg
     8581 gcctttatta cagagacgac tggcaaatgg ggtaaagccg aagtacggtt tgggtttaac
     8641 ttgtccaggg ttttccagat aaaaaagaat aagaaggcaa atgagtaact ggcggttgaa
     8701 tttttatata gaatgtaaat caaagtgccg tgataattca cggcacttta tttttgctca
     8761 aattctaata atgatagctg aattatttaa tcaattggcc cggagccgcc ggtctgtttt
     8821 tcccgaccag tttgaagcag gaaaaaaagt ggatgatgcc attataaaag agatactgac
     8881 caatgctacc tgggcaccca atcacggaaa aaccgagccc tggcaattta ctgtattcag
     8941 cggtgaagga ttaaataaac tggcagcctt tcaaagtgaa ttgtataaag aactggccgg
     9001 cgatagtttt aaagaactta cttatacaaa acttcagcaa aaccccctca aggcatcaca
     9061 tattgttgct atcggaatga aaagaactac aaataagaat attccggaag tagaagatat
     9121 agaagcagta gcctgtgcag tacagaatat gtatttatca gttacagcat atggtctcgg
     9181 cgggtactgg acaaccggag gtgtaactta taaagaagag gccaagtctt tttttggttt
     9241 aggcgaattg gataagctgc ttggtttctt ttatattggt tatatcgcta taccttcgaa
     9301 aggtgcaaca agattaccat tggaagaaaa agtaaactgg ataaaagaat agcatatgcc
     9361 aaaacaaccc gttacaccac cgtaccatat ttactatcct gttaaagtgg caaagagcaa
     9421 aatagccggt aagggtgctt atgccttaca gactattccg gcaaagaaaa agatcggtga
     9481 tcttggcggt gttattatta ccatgaaaga ggcaatgaaa ctgatcaaaa acctgaaagt
     9541 aatcaacctt gtggaactgg ataatgatct tgcattaaac gcatctgcca aaccaaacga
     9601 tatgcgtttc attaatcact cctgcgatcc caacacctat ttgagggtaa tgaaaaatag
     9661 agtagagttt tatgcattga agaaaattaa gaaagggcag gagctgagtt gcgattacgg
     9721 agaaacacac catgagggga ctttgccatg ccggtgtggt gcgaaaaatt gccgtggttt
     9781 tatctgacat gatattaatc agctaataaa gtattccggt tccttttctt tgagctttac
     9841 tataactttc atgaaaaaat attccattat cagccttgta tctgcattgg ttcttttttg
     9901 tatttccttt acgaatgaaa atttaagaag tatttattcc cgtccacctg ctgaatggcc
     9961 aaaaccgttt gtagatgatg gtgtgcagtg gaaagaatta ggaatgctgc ctgaaagccc
    10021 cgttgaaaag tataaagatt cactgatgca tattattaaa ttgggcaaga cacttttctt
    10081 tgataccagg ctcagcggtt cagggaaaat ttcctgcgcc acctgtcacc agccggagct
    10141 aaactggaca gatgccaaag aaaagtcaat cgggcatgag ggagcaataa ataaaagaaa
    10201 ctctccaacc atacaaaaca gctggtttta taaccggtta ttctgggatg gcaggtcggc
    10261 cgatttgcag gaccaggcat ttgcccctat caacagtgaa acagaaatgc acagtgaaat
    10321 gcccgatgta atacgaaaac tgaggaaagt aaatgggtat aaagaattgt ttaaaaaagc
    10381 gtttggtgat gaaaagattg accccgaccg gttgacggaa gcaattgccg tatttgaaaa
    10441 aacgatagta agccggaaga gcaggttcga tgaatttttg gaagggagta agaaggcttt
    10501 aacgaacagt gaaatccggg gattgcattt attccggaca aaagctaaat gcatgaattg
    10561 tcatagcgga cctttattaa ccgataacct atttcacaat agtggctttt ctggaacaga
    10621 tgaagggtat tataaaataa cgcacctgga agaagatagg ggtaaattta aaacaccctc
    10681 tttaagagac gtaatgaaaa caggtccatg gatgcatgac ggaaagcaaa acaacatgtt
    10741 gcagattatc gagaaattca atacagggga tatgccagtt ggtaaggata aactgattca
    10801 gccattaggg cttacaaaaa gggaaaaaac tgatttactg gctttcttac aagccatttc
    10861 agccccgcca ctggaatttc aaaaaccagt tttacccgac taggcaacta tttcagtcga
    10921 aaagctatct ttgctatcca tttaataaat caatcaaaaa tgatcaatat gaaaaaaatg
    10981 ttctttacgc tggtgatggc tggttttgga tttattgtca atgctcaacc acctgctggt
    11041 gatgcaaagc ccggtgaatg gtatggcgaa aaaatatcca cagagggtgc cctcaatatc
    11101 aatgatgtag tggcaaaact gaatggcggt tcagaatttc ctgaagtgaa aataaaagct
    11161 aagattacgg aggtttgtcc taaaaagggt tgctggttaa aactcgaatt ggaaaatgga
    11221 gaaaccgcaa tggtaaaaat gaaagattat ggttttttcc ttccggtgtc tgcgaaaggc
    11281 aaaaccgttg tgattgatgg aaaagtggaa atcaaaacta cttctgtagc agaactgaag
    11341 cattatgctg aagatgccaa aaaatcaaaa gaagagattg atgcgattac aaagccggag
    11401 aaagaagttc gggtgatggc aaaaggaata gtaattgctg aataaaatag ttcgactgtg
    11461 aatattgtaa aggctgccta tttaggcagc ctttcttttt ttaataaact cctttcttgc
    11521 caatttccgt tttgcgtaag cagagtttct gattgtgaaa atgaattaaa caagcctggt
    11581 tgatctttgc ggtctaaatt aagatataga tgcttaggag aatgatgtca atagtagccg
    11641 taatggtcat agctccggtt atttttgccc aggacagtac aacaatattt catgatgatt
    11701 caaccaaacc ccgatctaaa tatttggctg acgtaacagt cgttggccgt aacagcaaaa
    11761 gcgattatca gcaaatgccg gagattgttg gtacttctat ctatgccgga aagaaaaatg
    11821 cattgatcgt tttggataat gtacaaggta atgtggtaac aaatacgatg agacaggtat
    11881 tggcaaaagt gcctggtata catgtatggg aaagcgatcc cagtggtata cagattggta
    11941 ttgccgcaag ggggttaagc cctaaccgta gctgggaatt taatatccgc caaaatggtt
    12001 atgacatagc agcagatcct tttggatatc ctgaagctta ttataaccca cagttgcagg
    12061 cagtgcaacg tattgaagtg gtgagagggc agggcgcctt gcaatacgga ccgcaattcg
    12121 gggggcttgt taattatatt ttgcggaatg gaagtgagat cagtaaaaag tttgaattcg
    12181 aaacacagca aactgttggt agcaatgctt taattaatag ttacaatgca atcggaggaa
    12241 agagtggtaa gtggcattac tattccttct ttgatcatcg caatggagat ggctggagac
    12301 aaaacagccg ctatttcact aatgcaggtt acggtacagc tacttattac tttaatccta
    12361 aactttcgct gactgcagaa gttatgttct ctcatatccg cagccagcaa cccggtggat
    12421 taacagattt gcaaattgat ctggatgcac agcaaagttt tcgaagccgc aattggtttg
    12481 atattaaatg gaccactccc gcccttatct taaattacca gctcaatgaa agtactaagt
    12541 ggaataccaa actatttgtc accattggca acagaagcag tgtcggtttt ctgcaatcca
    12601 ttacagttaa agacagtatc aattccggta ccggcgaata caacaacagg accgtacagg
    12661 tagataatta tcgtaattac ggattggaaa gccgtatcat caccgattat aagattggta
    12721 aattgcaaaa cacactgtcc ggtggtattc gtttatacac cggcactacc caccgcatgg
    12781 cggatggaaa tggaacaaca ggatcttcct atgatgtaag cataattggt aactacccga
    12841 gagatattaa cttcgattca aagaatgctg cagcttttgc agaaaatatt ttccggttca
    12901 gtgataaatt ttatatcata cccggtattc gtttagagtg gttagaaggc tctgcctccg
    12961 gtcgcaatgg ttatacttct ggaggtgttg agatattatt gcagaatatt aaccgcagca
    13021 gaagttttgt actcgccggt attggtacag aatatcatat tacaagaaca acagaaattt
    13081 acgctaacat ttcacaggca taccgtccta tccagtttgc taacctgcag gctccgccca
    13141 caacggatgt ggttgaaaat gacttgaaag atgctaaagg ttttaatgca gaccttggtt
    13201 atcgcggcaa agtgaaaaat tatctgcagt ttgatatcag cggatattac ctgcattaca
    13261 ataaccggtt gggtacagta acagtaagcg gcaccccttc ataccgcctc attactaatg
    13321 tgggcagcag taccagcaaa ggttttgaag gttatgctga gttcaacccg tggagggcat
    13381 ttacccaaaa taccaatgca gatatcattg tatttggttc ttattcctac actgatgccc
    13441 gttacagcag tgatcataaa gatgcagcta ctaaaggcaa gaaagtggag aatgcaccac
    13501 agaatatttt ccgtggcggt attacagcgg gttacaaaac attcctgctg acaacacaac
    13561 tgagctatgt gggtgaagct ttcagtgatg caaacaatac cctggcacca accgccaatg
    13621 gcaataacgg gttgattcct tcatataccg taacagatat cacggccact tataaatttt
    13681 ccaaaggatt gaacatcaaa acagggatca acaaccttac tgatgcccgt tatttcaccc
    13741 gccgtgctgg aggataccct ggcccgggtg cattacccgc tgatggaaga actttctttg
    13801 taagcatcgg agctaagctc tgataccgat aacagctgta aagcgcattg ggtaaatcaa
    13861 ttattgaatt atccaatgcg cttttgtttg gtacatatat gatgttcatc agtaagttga
    13921 ctgattgcaa aacatgcgtt gtcatctttt tgtcaaacgt agtttcattt acatttcctg
    13981 acaaacaggt gataaatctc atttattgct gaagaaactt tgcgcagcaa taaaaaaatt
    14041 atgagaaaaa ttcacctctt cctcgttgca gtactgatca atgcatctgt acatggccaa
    14101 ttggttaaga ttgctcctcc ggaggacacg acaaaagcca aagaacttga tgaagtagtt
    14161 attacaggtc agtacaagcc ccagtctgtt aaaaactcag tataccaggt tagagtaata
    14221 ggaaaagagc ggatcctgaa acaaggtgcc acccgcctgc aggatgtatt gaagaatgaa
    14281 ttgaatatgc gtttttcgca ggatgtggct actggtggtt ctgcgatctc tatgctgggt
    14341 ttaagtggcc agaacgtaaa aattttgatc gatgggctgc ccgtcaccgg tcgccagggt
    14401 gtgaacaatg aatttgatat cagtcagatt gatatcaatt caattgaaag aattgagatc
    14461 gtggaaggcc ccatgtctgt tatttatggt gctgatgccc tcgctggtgt aattaacatt
    14521 atcaccagga aagctgcagc atacaatttg tctgtgaatg caagaataca tgaagagaca
    14581 gttgggaacg aatttggtat ccatcaaggc atccataacc aacaggcagg tcttacatgg
    14641 aaaaaaaata aatgggagat tggcggttcg tttgcacata attacttcgg aggttggaaa
    14701 gatacggcca ttgaaagaga attggtatgg cataaaaagg accagcttat aatgaatggc
    14761 tttattgggt atactaatgg caaactgaac attcgatacc gtgttgacgg attagatgaa
    14821 gtgatcacca atcccggcaa tttcagtcct tttgctgacc ccatctctgg cgatacgctg
    14881 gcgtttgacc aggaatatct ttctcaacgc ctgatgcacc agttgcaggg ttcttatttt
    14941 gttaatagca gtttgtcatt ccaactacaa tcttctttta ctgattacag tcgacaggtt
    15001 ttttcaacga ccatcaataa aaagaatgga gatatccggc ttgatccggg agaaggacgt
    15061 cagtcggttg ttgactttac cggctttaca ttccgcggta cagccttata taaattatca
    15121 gatgtattca gtttccagcc cggtgttgat ataaatttag ataaggggct tggtgaaaga
    15181 ttgaaaagtg gatcaaatgc tgtgaatgat tatgcattgt tccttacaac tgagatcact
    15241 ccttcttcta agatcaacat tcgtcccggc ttacggttta taagaaattc agtgtatgat
    15301 gcgccaccgg ttattccttc aatcaatacc aagttccagt taacacaaag cctggacctg
    15361 cgtttgtcct atgcccgtgg cttccgttcc ccttctcttc gtgagttata cttcagcttc
    15421 ttcgatgcta atcaccaggt ggtgggtaat ccggatctga aggctgaaac ctcgcatagt
    15481 tttaccggat cattgagctt taagaaaaaa agagtagaag aattacagta ttctgttcaa
    15541 ctgggaggtt attacaatga tgtaaaaaac atgattgact atgtctttac tcaaagttct
    15601 gatacagcaa gattgtataa tgttttcagt agtaaaacat caggtgctaa tctgagcact
    15661 gttgttacct ataaaaaatg gagtttctcc gctggtgctg cctataccgg tttttataat
    15721 gaacagtacg aaacaaataa agacctgcct gcattggttt ggtcagctga agcgaatgct
    15781 tctgtggggt attctattcc tgcaattggt ttagacctga acctgtttta taaattaaca
    15841 ggtaaacgtc cccgctatgt gatggatggt cctgatgtgg tgttgttaga gcaggatggt
    15901 tttcacctgg cagatttcac cctgaataaa aaattgacca ggagtctgac gctgaatgca
    15961 ggtatacgca accttttcga tgtagatcgt atcaacagca gttttgttca gggaggaata
    16021 cataccagtg gtggactgaa tgttgccaca ggccgttcct acttcgcctc ccttcttttt
    16081 aactggaata aaaaataaat aattcaggta aacataaaca gatttcaaga tgaaacagaa
    16141 atttataagt atatcagtag cttttctttc tttgttatta ctcgtggcag gatgccggaa
    16201 gagagatgcc gatttagctg ataacctcgt tgtatttgaa acatcagcac aaggtattac
    16261 cgcttcagaa aattctatta ctgttaagct taaattagtg agaggtacag acagagatat
    16321 tccggttact attaacctgg ttgaagaagg ggtagtgtat ggaacagatt atacgaccac
    16381 accggccgct gctgcaggta aactgacagt gactattccc tcaggaaata atgaaacatc
    16441 atttacactc acaaagaata gtggtgtttt atttgatggt gatgaaaaga tagtttttga
    16501 tctgttcagt tcgggaacac ctgtttttat cggggttgcc aggcaattca cactcagttt
    16561 tgctgaactg gtggctgcta attcaacact gacaataaat ggagggggtg ttttatattc
    16621 aaacaaagta ttcattgatc ttagtgccaa ccgtcagact gctgtaaacc gaaatacatg
    16681 ggatcttggt ttttacaccg gtgccgatga ttaccgggtg attttaaact catcctctgc
    16741 tatgatggca aaacagatca ataagaatga tcttacacaa gtaacagctg ccgatacggt
    16801 tggattttca acagaagtag cttttaacca gtttgcacct gcaactattt cattacctta
    16861 tatagattac cccaatggtg acctcgcaag aacagctatc atggcaacca gtgcagcagc
    16921 tgcggagaat aaagtctata tcgtaaatcg tggcctggct cccggaagcc ctgcaccggc
    16981 aaggggctgg aaaaagatca ggatccttcg taatgcaagt ggtgggtata cattacagca
    17041 tgccgatatt gccgctacca ctttttcaac tatagatata gccaaagaag aggcttattt
    17101 ctttaaatat atttcttttg acaacggtgc cgttagtgta gaacctgcca ggaagaaatg
    17161 ggatattgcc tggtcttatt tttcaaacgt caccaatttt ggtgcaggcg aagtacctta
    17221 tctttttcag gatattattt tgctgaacag gaatgtgcag gtagccaaag tgatggccgg
    17281 taccaggaca tttgctgatt ttgggatagg agacatcgca tctcagactt ttttgaccac
    17341 acaaaatgct attgctgctg actggcgttc aggtggtggt ccgggtacgt cacctgcggt
    17401 aagaacagat cgttattata ttattaaaga cggtgataat aattattata aacttcgttt
    17461 tactgcgctt acgcaaaacg gggagagagg ctaccctgct tttgaagcag tttggctgaa
    17521 gagagattag gttaactggt tataggatta aaagcccctt tttaaagttg ctttttttgc
    17581 ttttttagct cataaaaagc tttttttatt ccgtaacttc gccaccattt tagaatggct
    17641 atgagtgcta aatacagaga atttacggga ttgaacctgc cggctttcga gcaggaggta
    17701 ttggctaaat ggactgaaag ccaggctttt gaaaaaagtg tatcccttcg tgaaggggcc
    17761 actccctttg tattttatga aggacctccc agcgccaatg gtatgcccgg aattcaccac
    17821 gttatttcaa ggactctcaa agacctggtc tgccgctata aaaccatgaa gggtttccag
    17881 gtgaaacgaa aaggtggttg ggatacacat ggtttgccga ttgaactggg agtggagaaa
    17941 gaactgggca ttaccaaaga agatattggt aaaaaaatct ctgttgaaga gtataaccaa
    18001 aaatgccggg aagcggtatt acgatacaaa gacaagtggg atgagattac ccgcaagatg
    18061 ggttattggg tcgatctgaa tgatccttat atcactttca agaatgaata tattgagacc
    18121 ctgtggtggt tgctgagtga attgtacaaa aagggattgt tgtacgaaag tgtaagtatc
    18181 caaccatact caccggccgc gggaacaggt ttaagctcac atgaactgaa ccagccgggt
    18241 acatataaag atgtgaaaga tacaagtgct gtagcgatgt tccgggcagt gcaggatgaa
    18301 aaaagtaaat tcctgcatga tgtggtacat ggtgcagagg tattcttttt agcctggaca
    18361 actacaccct ggaccttgcc ttcaaatctt ggcttgacgg ttggtgccaa tattgattat
    18421 gtattggtaa aaacttttaa tccatacaca catttaccta ttaatgtggt gcttgcaaaa
    18481 gcattattgg gtaaatattt caaggaaaag ggagagaatg gtgattttgc cggctacaat
    18541 gaaaaagtaa agatccttcc ctggaaaata cttactgaat tcaaaggttc acaaattgaa
    18601 gaatgccgtt acgagcaatt gcttccttat gaagccaaca ctgctgctca tgcaggtggc
    18661 gatccgttcc gggtactggt agatactttt gttaccaccg aagatggtac cggtattgtt
    18721 catactgctc cagcttttgg tgcagatgat tataaggttg ggaagaaata taatatcggt
    18781 attcttacca tggttgacag ggaaggaaaa tttgtagatg gattaggaga gttcagtaat
    18841 cgttatgtga agaactataa agatgaccca aactacgttg atgtaaatat tgacatagcc
    18901 gtaaaactga aaaaggagaa cagggctttt aaagttgaaa aatacgaaca cagttatccg
    18961 cattgctgga gaacagataa gcctgtttta tattatcccc tggatgcatg gttcattaag
    19021 accacagcta tcaaggaaag gatggttgaa ttaaataaaa ccattaactg gaaaccaaaa
    19081 tcaaccggcg aaggtcgttt tggtaactgg ctggagaata tggtggactg gaacctgagc
    19141 cgtagccgtt attggggtac acccttgccg atctggagaa cagaagatgg agaagaagag
    19201 atttgtatcg ggagcatcga agaacttaat accggaatta aaaaggcgaa tgaaatatta
    19261 ggtgttgctg ccaataagaa ctacttgcat gaagggatcc tcgatcttca caaaccgtat
    19321 gttgacgata tagttttggt cagcccttca ggtaagccta tgaaaagggt tccggatctg
    19381 atcgatgttt ggtttgacag tggtgctatg ccttatgccc agtggcatta tccctttgaa
    19441 aataaagagg acttcaaaaa atcatttcct gcagacttta ttgcagaagg agtggaccag
    19501 acaagaggct ggttctatac gcttcatgct attgcctgtt tattatttga ttcagtagct
    19561 tacaaaactg ttgttagcaa tggtttggta ctggataaga acggcaacaa aatgagtaag
    19621 cgattgggta acgtggtgaa tccttttgat accattgaaa agtatggagc ggatgccacc
    19681 aggtggtacc ttatcactaa cgcttcaccc tgggataatc tgaagtttga cagtgaaggg
    19741 atcaaagaag tgcaacgtaa gttctttgga acattatata atacttacca attctttgca
    19801 ctctattcaa atgtagatgg gtttgctttc aaagaggaat acattccttt gaaccagaga
    19861 ccagagatag acagatggat tctttcttca ttgaacacgg tggtgaagaa agtgaatgat
    19921 tacatggatg attacgaacc tacacaggca ggtcgtgtaa ttgaagactt tgtagatgag
    19981 catttaagta actggtatgt gcgtctttgc cggcgccgct tctggaaagg agaatatgaa
    20041 caggataaga tatgtgccta ccagacattg tatgaatgtt tggaaacgat aacaaggtta
    20101 attgcgccga tctccccgtt cttcagcgat gctgtttttc aaaatttaaa tgctgtaaca
    20161 aatcgtttta aggcagaatc tgttcatcat gtcaactttc ctgtggctga tgtatcagtt
    20221 attgatgaat acctggaaga aagaatgcaa ctggcgcagg atgcatcttc tctggtctta
    20281 tcacttcgca aaaaagtaaa tatcaaggtc cgccagccat tgcagaaagt tttgatacct
    20341 gtattgaatt cttccatgaa agagcaatta caaaaagtgg aagacctgtt aaaggcggaa
    20401 gtaaatgtga aagaaataga atacctgaac cctgacaata cttttatcag taaaaagata
    20461 aaacccaatt ttgtagccct gggcaaaaaa ctgggtccta aaatgaaagc ggtatccaca
    20521 gctttatcac aattcgccca ggaggatatt gcccggttgg aaaaagaggg tcaatataat
    20581 ttgtcaattg atggcgaacc tgtaatatta caaatcactg atgtggagat cagcagtgag
    20641 gatattccgg gttggacagt ggccaataaa ggcaatttga cagtagcatt ggatgtaaca
    20701 gtaactccgg aactggaagc tgagggcaat gccagggagt ttgtgaaccg gatccagaaa
    20761 atcaggaaag acagtggttt tgaattgatg gacagggttg aaattaaagt tgcagcacct
    20821 gatggattaa aagattcgtt ggctcatttt aaaacctata tttgcgccga aattctggct
    20881 gataagcttg agtttttgac cgaaattgag gatggaattg agatagatat taacgatgat
    20941 ttgctaaatg tgattgtttc aaaaaaaggg taaaaatatg gctataaaga aaaaaaaggc
    21001 tacaaaaaag gccgccaaac ccgtaaaacc cgcaaagaaa gctgttgtta aaaagccagc
    21061 agcaaagaaa actgtgaaaa aatctgcacc caaaccagcc cccaaaaagg tggcaaaagc
    21121 ggctccaaaa aagcctgtaa aaccagttgc aaaaaaaccg gctcctaagc ctgcaccaaa
    21181 agctgcaact aagccagttg ccaaacctgc acctgccccg gcaccaaaac aggaaccccc
    21241 tgcccccaag ccagtggtta aaaagcatgg aggtattgat cctaaatctt tagtttcaat
    21301 caaaccaaaa gtgcagccag tcattaagaa ggagccgccg aaaccatcga aaatggtgat
    21361 tccaaaaaca agtacaaaaa gctcggtaaa atatgaaccc gagtttacta aatccgtatt
    21421 agatacagct atgcagcaat attccggacc aactatgaga tattcagacg ctgacctgac
    21481 tgagttcagg ggaatcattc agcgtaaact agacgcagca aagaaagaat tagcttattt
    21541 acagggcttg ataacccgta aagaagaagg tggagatatg gatgagggcc gctacatgac
    21601 aatggaagat ggaagtgtaa gtatggagag agaacaactc agccagatgg caagccgcca
    21661 gatcactttt attgatcacc tggaaaaagc catgatgaga attgagaaca agacctatgg
    21721 catctgccgt gttacaggta aattgattga taaagcccgt ctccgtgcag tacctcatgc
    21781 aacactgagc attgaagcta agcaaatgat gaataaataa gttttctgaa aactaacgat
    21841 aaacaaaacc ccgggtaacc ggggtttttt atttgttgca aaagttctta tttctttccc
    21901 ctcaaaaaga tctggaaccc ggcaccaatg gtaaagccat ggttggtaaa cttggtagtg
    21961 gtttcattct ctgctcttgt atcaatactg ccatcaatac catcatcacg cagggttgtt
    22021 cttttgaagg tgtttttgtt gtacgaataa ttatatccaa tataaaaatc aagacctgcg
    22081 ttctcgccaa ccagtttggt aaatccgcct gtaaaggctg cattggcaaa aaatccacct
    22141 gaagaatttc catcatatgt ttgcttgtaa gcagatgagc cagaacctcc gtagaaaaat
    22201 ccttccgttt tcagactact gatacctcca ttaaggctga cctggccaaa aggcaggagc
    22261 gatccgttgc cggggaaata atttctggca aaacctccaa taccaatatt gaaaccattg
    22321 tttttgtcac tttgatatgt gctgccattt tgctcatagc ttgttttttg gccattggga
    22381 tttatattca ggttgatgcc tactacagta ttatcagaaa caaaccagcc cagggagggg
    22441 atgatcctga catcataact ggttattttg gaagtattgc ttcctattga aactaccgtt
    22501 atatcggaac tgccggaatt gaacaatacc gaaccaacag acgagcctgc cattctatct
    22561 ccctttttga attgtgcatg ggtgcatatg gatagtaaga aaacggccgc aaaaaatcgc
    22621 attttaagtt gcatattatt gaattggttg gacaacaaat atagttgttc agggaaacca
    22681 cttgctgcta tttttttatg aaacttgagg tgattttgaa attttttcct ttacctttaa
    22741 aatatcttgg taaacaggaa aattcctttt acttttaaaa ccgaaactaa ttaacagcca
    22801 aatcaaactt tatgagaaag ttcagactac tctcgctatt attcttagcc atcacattta
    22861 tagccgttag ttgtacaaaa gaaggtcctg aaggtcctgc tggtgctacc ggtccgcaag
    22921 gacctactgg tgctaacggt gctaatggcc ctgctggtcc tactggccca actggtcctc
    22981 aaggcccgac tggtcctgtt ggtccgcaag gtcctgctgg tcctgccgga actgcaaatg
    23041 ttatttattc aagctggttc ccaatcctgg gtacagattg gactggttca ggtactgcta
    23101 accaatctta cacattaact gcccctggtg ttacgcaggc aatacgagat aatggtgttg
    23161 tgctggtgta tgtaacttat aataacctga taagaccatt acctttcaat gatgtctcaa
    23221 ctgctaatcc tattacattt gatttttatc tgccagcaac tgttggacag atattattaa
    23281 gagcatatcg tcccagttcc gctttcacgt tgattacaat aaatgttcgg tatgtaatta
    23341 ttcctggtgg tgtggccggt ggtcgtacaa tcggaaaagc tgcagaaata aacgggcaag
    23401 tttatacaga atcacaacta aaagcaatgt cttaccaaca ggtatgcagc ttgcttaaaa
    23461 tacagccata ataaatttaa atctgaatag aaagagccgt tcccatatgg agcggctctt
    23521 tgttttagca ttattttctg tattaccttg tgtctccaaa aggatctatt atgaagaata
    23581 ttgctttact cttattttgc ctgctgttaa ctattataac aattgctcag ccccgtatca
    23641 tatccggacc tatgcttggt ccggttgaat taagggatgc aaaagtatgg ctggaggttt
    23701 ctccggaagt aaaatccgtt tcattgcaat acaacaagaa gggagaaaaa aagaccaaaa
    23761 ctgttttata caaaggagaa ttgggtaatg agttcaatcc tctttatttt acaataggcg
    23821 gattagaatt taataataca taccagtata agatatgggt gaatggaaag gctactacat
    23881 acggtgggga gtttacaact aaagatctgt ggcaatggcg aaaacccata cctgaattca
    23941 gctttttaac aggatcctgt tcatatttta atgagcctgt attcgacaga cccggtactc
    24001 cctatgggaa agactcttct atttttgaaa ccatgtcaaa agaaaaagct tcattcatgc
    24061 tttggctggg tgacgcatgg tacaccaggg aggtagatta ttttagtgaa tggggactct
    24121 ggtacagggc cagcagggac agggctactc cggtattaca gaattttctg aaatctatgc
    24181 cgcagttggc tacctgggat gatcatgatt acggacctaa tgacattgga aaaaattata
    24241 tcctgaagga agccagccgg aatgttttca acaaatattt ctgtaaccct tcttccggtg
    24301 aaaacggcca gggcatctat accatgacca cctggggtga cgccgatatt tttctaactg
    24361 atgaccgttg gtggcgtagt gcagacagaa tgaaagattc tgttgatggg aaaccaaacc
    24421 ctgaaaaaag aatgttagga aaacaacaaa tggaatggct gaagaactca ttgttgtata
    24481 gtagtgcgac ttttaagata ataactgttg gaagccaggt acttaatcct gtttctcctt
    24541 atgataaatg gagggatttc ccggcagaat accaggagct gatggacttt ctgaaagaat
    24601 ataaagtaaa cggtgtgctt ttccttacgg gtgacaggca ccacagtgaa ataataaaag
    24661 tagacagacc gggtacatat ccgctttatg atatcacagt ttccccgctt acttcaggaa
    24721 cacattcatt cggtggtccg gaaaaaaata atgcttaccg ggtatttgga ttggatgaaa
    24781 agcagaacta tggtaaattt tcattcagcg gaacaagagg caacagaaag cttactgttg
    24841 aatttttggg agtgaaagga gaaaaattag gtgagtggag tataactgaa aaggaattga
    24901 aaacccctca ataaatttgc ctcatgacta tttattttgg atacgagaag ggacaggtga
    24961 tccaggcttt gcgatatcat tttataagcc ggcaggagat acgcatcatg ctgatcctgg
    25021 taaatgtatt tgcattggca tcggttacat tatatgcgtt gggtaaaata acacctatgg
    25081 ctttctttat gggttcagct ctgtggatcg tattaatgat aagtttctgg tttatccttc
    25141 ctttcatggt ttaccggagg gctattactt tcaagcatga gtttagtatg gatttccggg
    25201 acgatggttt tacgctggca catgaacggg gctccaaatc atgggcctgg acagcactga
    25261 aaagctattt ggaaagcccc catttttttc atttatattt tgatagccgt tcattcttac
    25321 tcgtacctaa aaacggttgc aaagacagcg atgaagtatt tgagttacgt aaactgataa
    25381 aagagaaagt aaaaaaaggc tgattcagat aaggtggttt tgcagtggta ctacaatttt
    25441 ttctccatca catagtgtgg aatagtcact tcagtgaatt catcacctgt aactttatag
    25501 cccatttttt cataaaaacc gatagcattt ttccgggcat gcatggatag aatgctgtac
    25561 cccctgtccc tggcaatatt ttcagcaaag tgcatgatag ctcttccgat cccttttccc
    25621 tgaaggtcat tcaacacagc catttgccgg agccggacga tttccggatt ttcctctacc
    25681 agcatgcagc agcccagtat atcttcatct tcaaatgcgc caatgagtat attgcttttt
    25741 tcctgttcca actcattagt tgtaaagccc aatcccagcg gtttacgcag gatatcttcc
    25801 cgaagcttga ccatcttctg gaaatctggt gtaccgtgtt cgataatttt cagcgccata
    25861 actatgtaat taacgggagg gaagttcaaa atacaaataa cttataatac taccaaccgt
    25921 ttattggtat taaataaccg gctaaaagcc tttttcctgg ttattatacc ctctttttca
    25981 ggggattgag ataaaatggg aaaataatgt ctttccgatg cttatttatt caaaaagtat
    26041 atttttgcac ccaataaact aaactttaag atcatgtcag acatcgcatc aagagttaaa
    26101 aagatcatcg tagacaaact gggcgttgac gaagcagaag taacaaacga agcttctttt
    26161 accaacgacc tgggtgccga ctctttagac actgtagaac tcatcatgga atttgaaaaa
    26221 gaatttaata tctctattcc tgatgaacaa gccgaaacta ttactactgt aggtcaggct
    26281 gtaacttacc tggaagagca tgccaagtaa tgtataagtt atttgcatcc ggcagataac
    26341 aaaaaggcct gcctccgttg caggaaataa attaaacccc gccttcttaa tccggtatca
    26401 atcgggtgga aggcggttgg tttttatttt tgactaaact gctttatctt actattatat
    26461 ggaactaaaa agagttgtca taacaggtat gggggcaata acccctttag gcaatactgt
    26521 acaagattac tggaatggat taataaatgg tgtatccggt gcagatatga ttaccctgtt
    26581 cgatgcttca aagtttcgta ccaaatttgc ctgtgaaatc aaaggatttg aacctactca
    26641 gtttttggat aaaaaggaag caagaaagat tgaccggttt acccaattgg cattagtggt
    26701 tagcgatcag gcaatgaccg atgcagggtt aaataaagaa aatataaacc cggacagagt
    26761 aggtgtggta tttgccagtg gtattggcgg gttaataacc tttcagcatg aagtaacaga
    26821 ttttgccaga ggcgacggaa ctccccgttt caatcccttc tttattccca aaatgatttt
    26881 ggatattgct gctggtcaaa taagcatgag gcataactta cgtgggccca actttgctgt
    26941 ggttagtgcc tgtgccagta gtacgaatgc catcatggat gcttttaatc ttattcgatt
    27001 gggcaaatcg gatattatcc tgactggcgg aagcgaagcg gtgatcagtg aagccggtgt
    27061 tggcggattt aatgctatga aagccatgag tgaaagaaat gacgatccta aaacagccag
    27121 tcgcccttat gataaagatc gtgacggatt tgtgatgggt gaagcttccg gcatattggt
    27181 gttagaggaa ctggaacatg ctaaagccag gggtgcaaga atttattgcg aagtggcagg
    27241 ttgcggagcc acagcagatg cttatcatat tacggctcct cacccggagg gattgggtgc
    27301 aagaaatgta atgctggcgg ctctggatga agcagggatg aaaccggatg aaattgatta
    27361 tatcaatacg catggtactt ccacacccat tggtgacggt gcggaagtaa aagctattac
    27421 agctgtattt ggcgaacatg cttataacct aaacatcagt gctaccaaat ccatgacggg
    27481 gcactgcctt ggtgcagcag gtgtaataga agcaattgca agtatacaag ctgtaataaa
    27541 tgatatagtg ccaccaacaa taaatcattt tactgacgat cctgaattag acccaaaact
    27601 taatttcact ttcaataaag cgcagcaacg aacagttcgt gcagcactga gtaacacatt
    27661 tggttttggc gggcataatg cctgtgtgat tgtgaaaaag tacaaagcct gattcgtttt
    27721 tttttcgtag cttgattcct gtggattttt ttaagaattt ttttaccaaa cacgaaggtt
    27781 cttcttttaa aaaggaacta aagaatatgc tgggttataa acccgggaat atctctttgt
    27841 ataaaacagc acttactcac cggtccattc gtgacggagc tgatgaaaat aatgaacggc
    27901 tggaatacct tggtgatgct gtactcagtg ccttaattgc agattatctt ttcaaacgat
    27961 acccgtacaa agaagaagga tttcttacag aaatgcggag taagatggta aaccggcagc
    28021 agctgaatga gattgcaatc cgcatggggc tgaaaaaagt tacattatat aataaaatgg
    28081 atggttcatt aaaagtgagc cagatattcg gaaatacact cgaagcatta gtgggagcga
    28141 tctatcttga cctggggtat aagaaaacag ctaagtgggt gcttgaggtt atcattctgc
    28201 cccatatgtt catggacgat cttgaaaacc tggaaattaa ccacaagaac aagctatatg
    28261 gctgggccaa caaaaacggc cgtgtgctgg agtttgaaac gctgaatgaa aaactggaaa
    28321 acggccgtcg tctctttacg gttgctgctg tactggatgg aaagaatatt gccgaaggga
    28381 aagcttttaa taaaaaagat gcttcccaga ttgcagcaca ggtggcagtg gaaaagctgg
    28441 gaatcgttaa ttcggattca gttggtgagg aaccagatga gaaaaaagca caggcttaat
    28501 ttttcaatat caccatgata atatgtattt attaaagcga attgcattac tcttcctgtt
    28561 cattgctgtt tttattgcct gtaataatcc tgtaaaagga cgaaatgggg tagtttttaa
    28621 gaaccctgct gattacaatg attatattat tagccgtcag accatcctta ttaaaaatat
    28681 acttgatttg ggcaaaaagg cagattcaga tgttgattct gcttatggca tgttggattc
    28741 ttacaccaat gatgcgacaa gaattattgc agaaataaaa gacatgcctt cttataaagg
    28801 agattcttct ttacgggatg ctgctgtaaa ttcttttacc ttttataaga aattatttgg
    28861 ggagagttat aaaagggttc tttccatccg ccgggaggga ggagatgaaa ctgaagcagg
    28921 ggctgcagaa atcatgggaa taatggacag tattaaacgg gaagaagaaa tacatgataa
    28981 aagattacac aatgcccaga aagattttgc ttcccggttc aaaatgaaaa tgcgggacaa
    29041 tgaaattcaa aaagagatta atcagaatca gtagtaagtt actttttttc catacatagc
    29101 tcaaccagta ctccatttgt atgtttggga tgaagaaaac aaaccagctt attatcagca
    29161 cccagttttg gtttatcatt tagtaagaca aaaccttctc cctgcagtct tttcatttcc
    29221 gcttcaatat ccgtaacgtc aaatgcaatg tgatggaggc cctccccctt tttttctata
    29281 aagcgggcaa ttacaccctc tgggtcggta ctttccagca attcgatttt agtctccccc
    29341 tgcctgaaaa aagctgtatt tactttctca gtttcaacca tttctgtttt ataacaggga
    29401 gcgttcaaca atttttcaaa ttgggggata gagttagcca ggtttttgac cgcaatgcct
    29461 atatgttcca ctttttgcat attaagggct ttaatatcag ggctttcgat ttcggtaaat
    29521 tcagtgtgta acacgaaatt acccctttcc tttagaacct tttgagctaa ttttgtgttt
    29581 attgaaacga acaacgacta attcatttta tgctaaaatt tccgatttat ttagaccata
    29641 atgctacgac accctgcgac ccaagggttg tggaagcgat gattccctat tttaccaaca
    29701 gttttggtaa tgcagccagc cgtaaccatc cttttggctg gcaggcagaa gaggcggtgg
    29761 actatgcacg ggaacaggtt gccaggttaa ttggcgccga ccccaaagaa ataatcttta
    29821 cttcaggagc cacagaggga gataacctcg ccataaaagg tgtctttgaa atgtatgcca
    29881 gcaaaggcaa tcatattatc acctgcaata tcgaacacaa agctgttctt gatacctgca
    29941 aacacatcga aaaggaaggt ggggaagtga cctacctgaa agtaaaaccc aatggcctgg
    30001 ttgacctggc cgaactggaa gcggcgataa agccgactac tattttgatt gccatcatgt
    30061 atgccaacaa tgaaataggt acagtgatgc caatgaagga gataagtgcc atcgcaaaaa
    30121 agcatggggt gttggttttc tccgatgccg tacaggcagt tggaaaaata cccgtagatg
    30181 taaataaaga cggaatagat cttatggcat ttacggcaca taaaatgtat ggccctaaag
    30241 gagtaggtgc attgtatgta cgccgtaaaa atccaagggt aaaagtgaca gcccagattg
    30301 atggtggcgg acatgaaaga ggtatgcgta gtggtacttt gaacgttccc ggtatcgttg
    30361 gttttggcaa ggcttgtgaa atttgcatca atgaaatgga agcagataca gaaagaataa
    30421 tcaaactgcg ggataagttg gaaaatgcgt tgttacaggt agaagaatcc tacctcaatg
    30481 gtgataaaga ctaccggttg ccgcatgtaa gtaatatctc tttcaaatat gtggaaggtg
    30541 aagggttgat gatgggtttt aataagaata ttgcagtttc gtcaggttcg gcctgtacgt
    30601 ctgcttcatt ggaaccttcc tatgttttaa aagcactggg acttggcgat gaccttgcac
    30661 atagttcact gcgtttcggt ttgggaaggt atacaaccga ggagcaaatt gattatacga
    30721 tagaacaagt aaccaacaca gtaaataagt tgagagaaat gagtccgctg tgggaaatgt
    30781 acaaagaagg aatcgatatg aatagtattg aatgggctca tcattaattt ttaacctcta
    30841 aattttttaa catggcatac agtgaaaaag taattgacca ctaccagaat cccaaaaatg
    30901 tgggcacatt ggataagagt aaagctaacg taggtaccgg ccttgttggc gcaccagaat
    30961 gtggtgacgt tatgcgactc cagatagaag ttgatgaaac cactgggctt ataaaagatg
    31021 ccaaatttaa aacttttggt tgcggctctg ccattgcctc atcttccctc gctacagaat
    31081 ggctgaaggg taaatcaata gatgaagcgg tgaccatcga taatatgacc attgtggaag
    31141 agttaaatct tcccccggta aagatccact gttcagtact ggctgaagat gctattaaag
    31201 ctgcaatcaa tgattaccgg aaaaagaatg gcctggaaga actgaagttt gacgaaagtg
    31261 taatccatta aaaagaaaat ggggtgtgga aaatgctgag cagggtaagc aaaagcctat
    31321 ttgcacttac cacaaccgtt taactactac ccggatttat gagtgtagga ttagataatg
    31381 caatttatgt aagcgataaa gcaaaagcca aggtacagca gttactggct gatgccggtg
    31441 tagctaatga cccttcttac ttccttcggg ttagtgtggt gggtggggga tgttctggtc
    31501 ttagttataa actggatttt gataatgaac aaaagccaat ggaccaggca tttgaagaca
    31561 atggagtgaa agtagttact gatctgaaga gctttttgta cctggtgaac actacactcg
    31621 atttttctga tggcttaaat ggcaaaggat tttatttcag taatcccaat gccagtcgta
    31681 cttgtggctg tggcgaaagt tttgcagtat aaattattaa accacgaact tgatattaga
    31741 aggtccctct cccgagggat tttttattag ctataacagg cgcaaagagc tgaaatatag
    31801 aagctttagc ttattttgca tcattgtcca tgcctatgtg gtctgtttgt aaaaaagaac
    31861 tccgccaatt tttcagtagc ctgaccggtt acattgcaat tatcgttttc ctgttggtaa
    31921 acgggctggt attgtttgta tttgaagata atattctcga ctttggttat gctacccttg
    31981 accggttctt tcaattagct ccctggattt tgttattgct cattccggcc attacgatgc
    32041 gcagctttgc tgaagaattt aaaacgggta cctatgaaat attgcaaacc cgtccgctga
    32101 cccggtggca gatcattggc ggtaaatatc tcggtagttt aatagttgtt tttattgccc
    32161 tgttgccaac catcatttat atcttctcca ttcagcgtct ttcttctaac gagggtattg
    32221 atatgggagc tacaatcggt tcttatatcg gtttattttt tttggctgcg gtgtttacgg
    32281 ctatcagcat ctgttgcagc agctttacca ataatgcagt cgttgcattt attgttagtt
    32341 tgattggttg tgccttattg tattatggtt tcagtgccat aagtcgtatg cctgcattgg
    32401 ccaatggccc ggattattat gtggagatga tcggtattga tttccattac cgtagtatca
    32461 gccgcggatt gatcgatacc cgtgatgtgg tttattttct aagtttcata ttcctgtttt
    32521 ttgcaataac gaaccgcaat cttttaaaga ggtaatatgc gaaagaaggt tgaaaaaata
    32581 tttgcttcaa aatactggtg gctgatgcta ttggtcgtct tgctgattgt caattatttg
    32641 ggttcggtct tccatgcccg tttagattta acaaaggaaa aaagatacac actcagcaag
    32701 gctacaatgg atatcctgaa aaaccttgat gatgatgtgc agataaatgt tttcttaaaa
    32761 ggggattttc ctgcagggtt taaaaaactg gctaatagta cggatgagtt tttacaatta
    32821 ttgaaagaca ggaatgggtc aaagattcat tatcgttttg tttcaccggt tgaagagata
    32881 ccaggtacaa aaatcatcta tggagattcg ttggttaatt taggagctat ccctattaac
    32941 cttacagtac aaaaaaagga aggagaaagc agtaatatca ttttcccggt tgcaattatt
    33001 gagtataaag gaaagcaatc cctggtaaac ttatacccgg gagccaatag tcgtatttcc
    33061 caggaagaaa taaacggtgc tgaatcttta atggaatacc agtttgtaaa aacacttgat
    33121 aagttaacgg cttctaaaaa acccggaata gcgtattctg tgggaaatgg ggaaccaaca
    33181 gacggaagaa catttatgtt ccggcaggct ttagctgatg attatgattt taaaattatc
    33241 aacctgcata accaaccccg gatccctgat ggtgttgatg tgcttatgat tgtaaaaccg
    33301 accattcagt ttacggagga tgaaaaatta aagattgatc agtttgtcat gggaggaggt
    33361 aaactgcttt gttttatcga taatttaata gctgaacaag acagtcttgc ttttaaaccg
    33421 gaaacaattg cctatgaccg taatttaaat cttacagatt tgtttttcaa atatggatta
    33481 cgtatcaata cggatcttgt catggatctg caatgtgatt tattgccatt tgtggtaggt
    33541 ggaacaaacg agaacccgca aatggaatta ttgagctgga actattatcc tatgcttaac
    33601 ccgacaaaca gcaagcttac aaaaggtctt ggatatgttg ggagccattt cgcaaactct
    33661 attgatacta tacaggtaaa tggtgtaaga aaagaaccat tgctggtaac atcccccaac
    33721 tcacggataa taagtacacc agccttaatc agtctcaacg agaataagaa tgtacccgag
    33781 gatgttaatt ttaagcgcaa tgaaattccg gttgccatgc tgctggaagg caggttcaca
    33841 tcacttttca aaaacaggat cagccgggca caattagaca ctttgtcatt aatcggagaa
    33901 gctttcagag aaacatctga agtaagtaaa attatcgtag cagccgatgg agatatactt
    33961 tttaatgatt tcttgccagg tgaaggacca aatgcagccc cggttccaat tcaaatgggg
    34021 tggaataaat acacctatag agaatatttg aatcagacag agactggtag atttttcaga
    34081 cctgttgcta atagagaatt cctgttaaat tgtatagaat accaggttag taatcccggc
    34141 atcagtgaaa caaggaataa ggatgtggta ttgcgcctgc tcgattctca aaaagtaaaa
    34201 gaacaaaaaa ctacctggca atttattaat atcgctttac cggtattgct ggtaattcta
    34261 tttggctgga tttatcagca ggttaggaag agaaaatatg cagcatagta ccggttatct
    34321 taacatttca taataatttt ctttaatgac agcagatcag attacctacc tcgtttttgg
    34381 aatagttttg gtattcgccc tggtctttga cctgggcctt ttaagtaaaa aggggcagaa
    34441 ggttacaatc agacaggctt tataccagac ttttttctgg gttgggttag cattggcctt
    34501 ttttgtattt gtatggattg aaaagggcca gggacttgca ttagaatacc tgagtgccta
    34561 cctcatggaa tggagtctaa gcattgacaa tatttttgtt tttattctta ttttctctgc
    34621 tttctcggta aaagaaaagc attttggccg tgtgttgttg attggtatcc tgatggccat
    34681 tattttccgg gtaattttta ttaccgtcgg tgttgcattg gtagaaagat ttcattggat
    34741 attgtatgtt ttcggtgcct ttcttgttta tacaggatac aaaatgttta cggcaacaga
    34801 cgatgaagaa tttgatccgc aggggagtaa aatttttctc ttcataaaaa agtttttgcc
    34861 gctggcacca catgatggta atggtaagtt tgtcgtaaaa gagaacggca agtccgtcta
    34921 tactacacta tttgtggtgg taataatgct ggcagcaatt gacctggttt ttgctctcga
    34981 ttcaattccg gctgtaatgg gtatttccag agaccggttg gtgatttata cctctaatat
    35041 ctttgctgtt ttggggttgc ggtctctttt ctttttgtta agaggagctg ttactaagtt
    35101 tgattacctg cagcagggta ttgctattgt tttggttttt ataggtgtta aaatgctgac
    35161 tgaacactgg ataaatcaat gggtaagtaa acaaatacag gtctttatct ctttgggtgt
    35221 gattatgatt tgtattacag gctcaatttt ttattccata ttcataaata aaaggggagt
    35281 accgccggag gttaaagacg atagtgtttc ctgactgata tattaattat tttgctgttg
    35341 aattattctg taagcaatat tgcccaaatt attcatgcag acgcaaccat agtaaaagat
    35401 ttgtctgctg aacatctttt gttggatagc cggaaagtat tttctccggg ttcatccatt
    35461 ttttttgcat tagttggatt tcgccgtaat ggccatgatt ttatccctga tttatataaa
    35521 aaaggagtta gaagttttgt agtaagtgag aaattcgaaa cagctgcata tccgggagct
    35581 aattttttgc tggtcaatga tacactggcg gcacttcagc aattggcagc tcatcaccgt
    35641 aagcaatttg ccataccggt tattggtatt acaggaagca atggaaagac cattgtaaag
    35701 gaatggcttt atcaattgct tcatacagat tataatattg ttcgtagccc caaaagttat
    35761 aattcacaga ttggtgttcc gttgagtgta tggcagatga atgaacatca tacactggga
    35821 atttttgaag cgggaatttc taagcccggc gaaatggata aactggagag aataattcag
    35881 ccaactattg gggtattcac caatatcggg gaagcgcata gtgagggttt tgaatcgatg
    35941 aaacagaaag aagaagagaa aaaaatatta ttcaggaatg ctactgagac aggtaagctt
    36001 gatgttgttg aagtgcgtta tcagcaatcc acaacaataa ttactgccaa agatcccagg
    36061 gctgagaatg aatttatttc tattgaaatt ccatttactg atgacgcttc tgtacagaat
    36121 gccactactt gctggaaagt aatgctgaca cttggctacg acaatgaaat tgtcaggttc
    36181 aggatgaagc agttattacc ggtggatatg aggttagaaa tgaagaaagg gatcaatcgt
    36241 tgtttggtta taaatgacag ctatagcgct gaccttagtt cattggaaat agcactgaat
    36301 ttcctggttc aacaaagtgc aggcactcaa cggacagtaa tcctttctga ttttttacaa
    36361 agtgctgttg ctgatgaaaa attatatgca caggttgttg aaaatttaca taagcataag
    36421 gtagacagat taattggtat cggcgggagg atatccaact ctttacggca tctgattaat
    36481 agagcggata agcgttttgc tgttgaatta tatccatcaa cagatatttt tttgcagcaa
    36541 tacaggtcat caatgttcaa agaagaagct atactggtaa agggggcgag gatatttgca
    36601 tttgaaaaaa ttgttcaatt gctggagcaa aaggcgcatc aaactttact ggagatcaat
    36661 ttgaatgcaa tagtgcataa tttaaaggaa tatcaaaaag tattgaagcc aacgaccaaa
    36721 ataatggcaa tggtaaaggc ttttgcatac ggaagtggtg gggccgaaat tgccgggatt
    36781 ctgcaatatc ataaggtaga ttatctcggt gtagcttatg cagacgaagg cgtggaactc
    36841 aggaatgcgg gaattagttt gccaataatg gttatgaatc ctgaagaaaa tgcttttgat
    36901 gttattgtgg atcataacct ggagccggaa ttatattctt ttgaattatt gaattcattt
    36961 gattcttttt tgcaaaagga aggaattcaa cactatcctg ttcacattga aatagaaaca
    37021 ggtatgaaca ggcttgggtt tagcatagct gaaattgaga aattatgcac cagtttaaat
    37081 tcaacttctt ctttcaaagt gcaaactgtt ttttctcatt tagtggccag cgaagatgct
    37141 ttacaggatc catttaccat taatcagttc aataaatttg gaatagcgac cactttgttg
    37201 caggagagat tggggtactc atttataaga catatcgcca attctgctgc tgctatacgg
    37261 catcagcagc tacaactgga tatggtgcga ttaggtattg gtttgtatgg agtggatagc
    37321 gccggatctg gtaaactgaa cctgcaaact gttactacac ttaaatcaac tatcgcacag
    37381 gtcaagcacc tggaagcagg agaatcggtg agttataacc gtaatggagt ggtaaataga
    37441 gattctgtta ttgccactgt gcggctgggt tatgctgatg gttacccaag aagactggga
    37501 aatggggttg gcaaagtttg ggtaaaaggt cacctggtac cagtaattgg tactgtttgt
    37561 atggatatgt tcatgataga tgtgacaggt attgacaata ttcaggaggg agaagaagtt
    37621 gtcatattcg gaactaattt accagttcag caagtagcaa aatgggctga aacaattgct
    37681 tatgaaatac taacaggaat ttcacagcgg gtgaaaagag tgtattatga agagtaaata
    37741 ccggaataat tttccaaatc aatgaccaca aggcgtttat caatgctaca tttatgactt
    37801 ctttgacctg acaccttgca gcattctcct tatatttgcc ccttaaaatt gtaccattga
    37861 agcttagaaa aatcatattc cttattgcag tgattatcat aattgaccag gcattgaaaa
    37921 tatggatcaa aacgggtcaa ccaaccggtg aagtagtgag ggtttttggg tttgattggt
    37981 tccgtcttca ttttattgaa aacccgggca tggcgtgggg ctggaaattt ggtaacgaaa
    38041 caggtaagat gattctgaca ttgttccgtc tggctgcagt gatctttggt acctggtata
    38101 tcggcagaat tgtaaagcag caatatagta aaggctttat agtttgtgct gggttgattt
    38161 atgccggagc tttaggtaat ctcattgaca gtatgttcta tggaatgata ttcgacaaag
    38221 gacttcatta tgatgctgct attaatgatt atattagtta tggtggtata gcacaatttt
    38281 cttcaaatgg ctattcatcg ttcttacatg gcagtgttgt agatatgctg tacttcccaa
    38341 taataaaatc aaattttcct tcctggtttc ctgtaatagg tggtgatgaa tttgaattct
    38401 tcagccctat ttttaatatt gctgatgctt caatttctgt gggtgtaatt acactgctga
    38461 tatttcaaaa gcggtttttt aagaagcatc atgaaaccgt tacttctccg acagtagaaa
    38521 ctaatactga aataagcgat aagatgcagg ttttgtaaag ggactattta ctaattactt
    38581 gttttcaata cacttttttg aataaactta ccggctgatt gtacccgcag gaaataaata
    38641 cctgtagtaa gcttgcttgt gttttcaaga ctcagtcggg taatcccgga agaaatatca
    38701 aatgcctttt ttcgtactga ttttcctgaa gcatcaaata attccgcttc ggctttcccg
    38761 ctattttgtg aagaaacttc gaataatagt tcattattga aaggattgat aacggataca
    38821 aatgataata tatccgctga tgaagccagt tgaattgttc ttgaatacgt tgactgtccg
    38881 gtattgtttc tcattctgat acggtaatac actttgccag ttagtttttc aggatcggaa
    38941 taggaatagc tgctttgttc ggagatatag tcattgtaac tgtttacaga accggcaaaa
    39001 ataaagttga tgccgtcggt acttctttct atgtcaaaaa ttatcggctc attttccccg
    39061 gtagttgtcc attttaatat agcatgttca ttctcttgtc gcccgctgaa agaaagtagc
    39121 cgggttttca gcgggatacc acagaagatt acccgaagtg taataatatt tccctggtct
    39181 gtaaaattgc aattcggatc agtcaggttg gaaagtgtag ttgcaacaac caccctgtac
    39241 atgtcaccat tattcggcat ttgtgtctgc gcaggggtta atgtatagga tataacatat
    39301 tcccaggcag taccgttcca aaatggactt gccggaccag ccggagctgt tacatcactc
    39361 caggtggcac ctccgtccgt actgcgttgc catttgtaat aagtatagtt attgaaatag
    39421 gaacgtacag tatcataaat agtaatcaca ttgctgtcac aaactgcagg gctcagggaa
    39481 ggagaatatt tcatgttagg agaacaggtt gaaactgaaa tatcatccag cgcccagtca
    39541 ttaccaccac cgcccggggc attattaaaa aacttcagtg taaaacttgt ttgtgcagga
    39601 ccagttaaaa aggtaaaacc ttttttaacc cattgcccgc tgtataagag atttcctgaa
    39661 gtataatagt cgataccgtc aatttcaaaa gtcaggttag gcgctacacc tgatgaatcg
    39721 ccggggccag ttggaatata aaagggaggt ccagcagcgt ttgtggcacc tttaccgttg
    39781 gaatcgcaac cgcatttgga acaaatattc ctcatccagc aggaaatttc gtaataggta
    39841 ttagggcata atgaggttat cgtttgctgg aaagcggaat cgatacgata agctgcgttt
    39901 ataaccagca tgtaacctgc gtttggatta gcaaccgtat ctgctgccgg attcccggca
    39961 ataggattaa cagcacccgt atggtcgccg ataatatccc ataaagtaaa gaccctgtga
    40021 cttgtcaggg ggcctgcgcc atccgggtcc ggatcaggct tgggccaggt atttaaagta
    40081 gtataacctg tatttgtaga agtattgtta gcgactccgt aataataatc ttgcggtgaa
    40141 ttaattccaa aggaattaaa tgtatatcct actggtacat tggctgacgt tcccctgtta
    40201 cggggctgtc cgcttccaaa tgtgccattg aattctgtac cgatagcatt tgtgccgatt
    40261 gtattggagc aaataccata gttgggatat actctgagtg tattagcagg aaaagtaaaa
    40321 gtttgtatag cggcagcccc atttttataa gttatattac cgccgccggt actgatattg
    40381 ctgcctgtag ctgcagtaac ccttatacgg aaagaagcga tcataataca agtgcttccg
    40441 taaaaagaag gtttatgtgt gttagctact ctgcctcttc taaaagctgt agcaggagca
    40501 gccccggttc taaacccgag attaatagta atagcggtac cggtaatcct gccctgatca
    40561 tcacctgctg catccgtaaa tgatttataa gggatacctt cattagtaag tactttaata
    40621 gtaccgggaa tataagtcgt tcctgccggt actgcatcct ggtagcggca gctgtcgtag
    40681 gtacccgaac gtactacaaa agaggctctg atttcaagag tgtcacctgt ttcgactgtt
    40741 cccccattca aaccttttgt tacgttgata tagcttttcc cgaagtcaat gttttgtgaa
    40801 taggcagaag ttattcctga aatcaatagt acaggaagaa gaaaaaataa aggtgaaaaa
    40861 atagtacggc gtaaattttt tttcatagtc tggattttca ggtacacagt tcggactcgc
    40921 tggtataaaa atagattaat gggtgtcttc aaacaataga agaaatgctt acttttcagt
    40981 tgtatttacg gtgtaatatt tactgtagat tccctattgt tagcggattt tcgaatggtt
    41041 acttttaaac ttaaatccat ttgcatgaaa aagccactgt gtgttatctt ccggatattg
    41101 gtgcttttat ttggtttctt tactgcagca gctcagaacg agccctttac gaaaagaact
    41161 gtcgtttctg gattaaattc tgcgtgggaa gtagtatatg gccccaatga ctctctttgg
    41221 gtaacagaaa acagggctta tttaatttcc aggatcagta tagcaaccgg agctaaaaca
    41281 gtgttggttg atttaagagc tactgatggt tctataaact tcacctcagg caccggaacg
    41341 cagccgcagg gtggtttaat ggggttggta attcatccaa acttatattc tgcagatccg
    41401 gcagtaagag cggctaagcc atgggtgtat gcagcatatg tttataacag gggaacttgt
    41461 ccgggtacta ataccagttg tattttcacc acaaaaattg ttcggtttga ttataacggt
    41521 aacacgttgt ccaacccggt aactattata aatactattc ccggcagcag cgatcacaat
    41581 tcaggaagat tagtaataag tccagttatt gagccgggtg ccgacgcagc gcatacacaa
    41641 taccggttat attatacggt tggagatatg ggggccggac aattcctcaa tactacccgt
    41701 gcacaaaatg cacagaacgt caatattatg gagggaaaag tattgcggat taattcagaa
    41761 agcgatggcg atgcgggcca ggatgcctgg ataccaaacg ataatccttt ttataacggt
    41821 gcagtaataa ctccgcaaga ttatgtcttt actttaggcc accggaatgc acaaggttta
    41881 gtatggggta ctgttggcgg gaataatatc ttgtatagca cggagcagat ggacagaaca
    41941 gatgatgaga tcaatataat tgaagccgga aagaattatg gatgggacca ggtatcgggc
    42001 tattgtgata ataacgttaa tgggtataaa attggtcaga ataccagtgc taatgaaggt
    42061 gctttttgtt ctgtaactcc cacgcaccag gaaccaattt ttacaacatt tacagaaaca
    42121 gcggctaata tgcctgcgct gatggcacaa tcgaataatc aactttggcc cacaatagcc
    42181 agttccagtg tcgatttcta tggtcagaac aagataccgg gctggaatta ttctttgctg
    42241 ataagtccat taaaaaagga tagaatatac cgtgtcagct taaatgcaac tggaactgat
    42301 gttataggcg ataccattcc ttattttcgt aacgacggga acaggatcag aagagtaacg
    42361 atttcttctg atggattaaa attttatgta gcccgggatg ccggagcaac tataaatgga
    42421 ggggctatta tggaatatac ttatgcgggg gttattcttt cgttgaatga aaatccaggc
    42481 gtaaggccgg taagagttga ggatttgatc agtatttatc caaatcctgc tacccgtact
    42541 atttatattc acggaaaaag agaactccgg aaacctttgt tggtgcagat ctatgatgtg
    42601 catggccgga tgttgaaaga agaaaccagt ttcagaaatg atttttcagt ggatatatcc
    42661 cgtttaattg cggggacata cattttgaaa ctttacaacg gcaatgatat ggaagtgcag
    42721 attgagaaat ttataaagca ataaatcctg aaggcaactg ctgttattaa aaaataacct
    42781 ccgcagtagc ggaggctttt ttttagggat tatgctcagg ggtttacttg ttatgattga
    42841 aattcctttc gcagttattt tctggttgaa gcactttctt taatataacg gatagctgca
    42901 gcccgatctg gattttctac aggtacagca ctttctgttc ctgatattat cctggaacca
    42961 gaaagaatag cttttaaata agcggatttg atctgttgtg aagacgactg tggttccggt
    43021 tttactggtt ttagggtgtt caacggaaag gaggaaataa gtctggcatt acttttcata
    43081 tggcggattt ttagttttaa aaaatgtatg gattaatgcg actgttaaga tagagacaaa
    43141 taaaaaccat ttgcaaatat tactggataa aaaagagaag aacgtggaat tcttctcttt
    43201 ttacgatagg aaaattacta tttcaattta ccaaccatat tggccggcac tacccactca
    43261 tcaaattgtt ccggggtaac gtaacccaac ttaactgcca tttcttttaa tgttgttccc
    43321 tgtttgtgtg ctttttgtgc aatttcagca gctttataat agccaatttt ggtattaagc
    43381 gaggtaacaa gcattaatga gttgtcaaca tgttttttga tattcgcttc aataggttca
    43441 ataccgactg cacatttatc attgaaagag acgcaaccat cgccaatcaa cctggcacta
    43501 tgcaggaaat tatagatcat taccggttta tatacattca gttcaaaatg gccattggca
    43561 ccaccaatac tgatggccac atcattgccc atcacctggg cggctatcat ggttaaggct
    43621 tcacactgcg ttgggtttac ttttcccggc atgatggaag agcctggctc attatccgga
    43681 atgaataact cgccaatacc acttctcggc ccggatgata gcattcggat atcattagca
    43741 atcttcatca ggctcacagc cactgttttt aaagcaccgt gtgcttctac aatagcatca
    43801 tgtgctgcca gggcttcaaa tttattttct gcagttacaa aaggtaatcc cgttaaagcc
    43861 gcaatatgtt tagctacatt cttatcataa ccagcagggg tattgatgcc agttcccacc
    43921 gctgttccac ccaatgccaa ttcacttaaa tgagccaaag aattgttgat tgcttttaac
    43981 ccatgatcga gttgagaaac ataaccgctg aattcctgtc caactgtcag tggagtggca
    44041 tccataaagt gggtacggcc aatctttacc actttcataa attgcttact ctttttggct
    44101 aatgtgtctc tcaatttttt tattcccggt atcgttgttt caaccaggat cttataagcc
    44161 gcaatatgca tggcagtagg gaacgtatca ttagatgatt gtgatttatt cacatcatca
    44221 ttcggatgca ggaatttttc tttgtcagtg agtttcccgc ctttcagtac atgaccccgg
    44281 taagcaacca cttcatttac gttcatatta ctttgtgtac cactgcctgt ttgccaaact
    44341 accagcggga aataatcatc cagtttgcct tttaagatct catcacacac tttaccaatc
    44401 agcaccgctt tattttttgc caatactcca gcttccagat tggtgagggc tgcagctttt
    44461 ttaaggtagg caaatgcccg gatgatctct ttaggcatcc ggttgatatc ttgtgcaatc
    44521 ttaaaattat caatactgcg ttgggtttga gcaccataat acgcatcagc gggaaccttt
    44581 acttctccca tcgtgtcttt ttcgattctg aattccatat aatttgattt aaggagtaaa
    44641 ttttgcgcag caaaggtagc ggaattggtt tgttcgggta ctgactttaa tcgtattcat
    44701 aaacgatttc atattatttc tttcgaggat taatcgtaac attgataata tgaatcggat
    44761 tgtccttttt tttatttttt tgtttattgc cggtagcccg atggcacagc agaatgctcc
    44821 ttcaccgcta cccaccaaac aacaattggc atggcacgaa aatgaatatt acctttttgt
    44881 gcattttggt ccaaatactt ttacagataa agaatggggg catggtgatg aaaaagaaga
    44941 aatttttaat cctactgccc tggattgccg ccagtggtgc cgggtagcga aagccgcggg
    45001 tgcaaaagga atcataatta cagccaaaca tcatgacgga ttttgtttgt ggcccagtaa
    45061 atattcaaca catactgtca gggaaagtaa atggaaaaat gggagaggtg atgtattgaa
    45121 agaattatca caggcctgta aagaagcagg tttaaaatat ggtatttata tttctccatg
    45181 ggacaggaat catcctgact atggtaccga aaaatacaat gatgtatttg taggaatgat
    45241 gaaggagctt tttcaaaact atgggccaat atgggaatta tggtgggatg gtgccaatgg
    45301 ggagggccct aatggcaaaa aacaggaata tgactggaga agatatgaga ataccgtaag
    45361 aaagctctct ccatctacag ttatcttcag cgatattgga cctgatatac gatgggtagg
    45421 caacgaaaaa ggcattgccg gagatcctaa ttggaatttt ttggatacaa caggatttaa
    45481 aagaggaatt ggtgcgccag atacagatac actcaatcag ggcaattatt atggaaagca
    45541 ctggatacct gccgaatgtg atgtgagtat acgtccgggt tggttctatc atgccaatga
    45601 agatcaaaaa gtaaagtcac cagagcaatt atttgacctg tacctgaaat ctgtaggaag
    45661 aggtgctaat ctcttattga atgtacctcc tgacagaaga ggattgatcc atgaaaaaga
    45721 ttcagcagca ctgatagggt ttaaaagatt gagggatgaa agtttttctg acaacctgtt
    45781 gaaaaaagca gatacctact atgaatttag ccaccgggat tttataaaaa agccactgct
    45841 gcttcgtgga tttgattctg cggctgcata ctacggtatt aatcttcaga attttattgc
    45901 agactttcgc cggccggtaa cagttaattg tattgtttta cgggaagcta tacatcttgg
    45961 acaaacgatc aggcgtttta acattgaatt atataacaat gacaggcttg tgggaaaagt
    46021 acagggacat agtgtaggac gtaagcggat actaactttc ccagctataa ctataacttc
    46081 attccgggtg aacctggaag acgcaaaggg taatgataat atcagcggtg tagctgctta
    46141 tttgatcaat gagaacctga tagaaaatga aaaataaacc aggcctacgt ataaaaattg
    46201 tattgacttc tttcctgctt tgttttagct ggatactgct ggctcaaaag caggaccggt
    46261 atatggaaaa attaatccgg gaaaatttta tgctggcagc tcagcaatat aaactacttg
    46321 ctgggaatac cccaaccaat gtgatgccga gaaattatat ggccaaagaa gggaaattag
    46381 ttacgagcaa taccacctgg tggacgagcg gttttttccc gggttcttta tggtatatct
    46441 atgagtttac caaagacagt gccatcaggg ctgaagcaga aagaaggctt gctatcattg
    46501 agcatgtaaa gcattatacc ggcgatcatg atttggggtt tatgatcttt tgcagttttg
    46561 gaaatgctta tcgcattaca ggtaatcaaa catataaaaa tgtgattgct gaagctgcag
    46621 aaacattaat aaagcggtat agatctacta ccaggtctat tcagtcgtgg ggtagaatga
    46681 aagattcaat aagtccggtg atcatagata atatgatgaa ccttgaattg ctaaactggg
    46741 taagtgatga gtggagagag cctaagtaca aggtaattgc aattgaccat gctaacacaa
    46801 cgttgaaaaa tcattttcgg cctgataaca gttcttatca tgtattggat tatgatgtaa
    46861 atacaggtaa tgtaataagg aaaaaaacag cacaggggtt tagtgatgaa tctgcatggg
    46921 cgaggggaca atcatggggg ttgtatggct ataccatgat gtatcgtttc actaaagaaa
    46981 gtagttacct ggagcaggca agaaagattg ctttattttt attgaatcat cccaatttac
    47041 cacctgataa aattccttac tgggattata atgcgaaaga tattcctgat acgtacaggg
    47101 atgtatcagc tggttctatc atggcttcgg cattactgga actggcacaa tatgaagtgg
    47161 atgctaaaaa gaaaaaggaa tatattgctg tggcaaaaaa aatacttcaa tcgctttcat
    47221 cggattctta cagaaacaaa cctggtgaga gtggtggatt tattttgaaa catagtgtag
    47281 ggtcacttcc cggcaattca gaagtggatg tgccgcttac ttacgctgat tattatttcc
    47341 tggaagcact gcttcgttat aaaaactggt acctgaaaaa gtgattttat ttgccggtga
    47401 aactggcttt tcttttttcc ataaaagctg ctattccctc tttcatatct tctgtggcaa
    47461 aagcattgcc gaattcttct atttccagtg cgtagccgtc tttggtttca ttaaatactg
    47521 cgttagctga tttgatgcaa cctgcaacag caagggaggc tttggaattt attatttcaa
    47581 gaattttacg ggtatgttcc agcagtgtct cgggagttgt tacatagttc actaatccca
    47641 attgttttgc ttcattggca tcaatcatat gcgcagacat taatagctcc atcgctttac
    47701 ctttcccgat caattgaact aatcgttgcg taccgccata accagggatt aatccaaggt
    47761 taacttcagg ctgaccaaat tttgcatttt ctgctgccac acgaaaatga caactcatgg
    47821 ccagttcaca gccaccacct aatgcaaagc cgtttacagc agctacaaca ggctttgaag
    47881 aattttcaat tctggcaaat gtatcttgcc ctcttttggc cagggccatt gcttcttccc
    47941 tgttcaatcc accaaattcg gaaatatcag caccggcgac aaatgctttt gagccggcac
    48001 cggtaataat cactgatttt atctccgggt tattttcaat ttcatccaat gctttattca
    48061 agtcggagaa tacgtctttg tttagtgcat ttaatttttc gggccggttg atcattatgg
    48121 ttaatatacc attgtctaaa attgcaagca gggtttgagc attcatatga gaatttttgt
    48181 aagccgaaga tagtaacatt cagttactgc tgctgttgca tttccataaa tcgccggtag
    48241 gaattgtcat tgaggcctgg attatttacc tggtaagaga atacaggttg tatagtctct
    48301 tcttctttta aggagctgtt atttattacg acagatactt cca
//
LOCUS       DAORTI010000014        45802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_465_length_45802_cov_6.828710, whole genome shotgun sequence.
ACCESSION   DAORTI010000014 DAORTI010000000
VERSION     DAORTI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 45802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45802
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_45802_cov_6.828710"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..3286
                     /locus_tag="PLO70_03590"
     CDS             101..3286
                     /locus_tag="PLO70_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ73568.1"
                     /translation="MRKLKRLAGAGLLLLLANVSISQPTFPENGVADPRHGHFAFTNA
                     TIVKDAGTTITNATMVVKDGKIVAVGSGLKIPAGAVEVDCKGKYIYPSFIDVYADYGI
                     PQPQRTGGGGQFNPSQQPQLETATKGAYGWNQAIKSDAEAYRMFAVDDAKAKPLREAG
                     FGTVLTHIKDGIARGTGAVVSLASEKENLVIIKERASAHYSFSKGTSTQSYPGSMMGM
                     IALLRQTYLDAQWYKNKPAAEGLNLSLKAWNETQDLPQIFEAGDKWNNLRADRIGDEF
                     GVQYIIKAGQNEYQRIKEMKGTNATFILPLNYPQAQDVEDPNDARFVSLQDMKHWEMA
                     PANAAAFEKAGIPFCLTTADLRSVNQFWANLRKAMEYGLSENKVMEALTKTPAITLGI
                     YDKVGSLEAGKLGNFLIANGPLFNEKTIILQNWVQGIKYTVKEDAASVAGTYSLTVTS
                     LSGKETYTLDVKNASAVTMFAKDTLNSKFSFDGKLVKISYAPMTRRQRPVMNPGQMPD
                     SVRRTGGGFPGRGGSGMEQALPVTATRLSGVSNGTEWNGTGTDSLGNPLTWTASLVKA
                     AEAKTDSVKKKDVPALGKIVYPFEPFGWDESQQPKQENILIRNATVWTNEKEGVLQNT
                     DVLIKNGKIAAVGKNLTEATARVIDGTGKHVTAGIIDEHSHIAAASINEGGQSVTSEV
                     RIADNLNPDDINIYRQLSGGVTTSHILHGSANVIGGQTQLIKLRWGANDDDLKLKGWP
                     GQIKFALGENVKRSNFSIPGGNNRYPDTRMGVEQVLIDAFTRAKDYQQDWKEYNTAKE
                     KLIKEKKSTASLLAPRRDLELEALVEILEKKRFITCHSYVQSEINGAMEVANKMGYTV
                     NTFTHILEGYKVADKMKKHGSFASTFSDWWAYKMEVEDAIPYNAAIMQKVGLTVAINS
                     DDAEMARRLNHEAAKIVKYGGVTEEEALKMVTLNPAIMLHVDDKVGSLKVGKDGDVVL
                     WSDHPLSIYAKSLYTIVDGTIYFDRQKDEQLQKEIDMERLRLVRKMNGEKRSGAPTIP
                     AQPSYQIMHTCSDHGHSHGLLVIDADDITNE"
     gene            3297..4628
                     /locus_tag="PLO70_03595"
     CDS             3297..4628
                     /locus_tag="PLO70_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ73569.1"
                     /translation="MRKIFIAISALFMIAANAQETVLPAKEQKGLLFIKNATIHVGNG
                     KVIENGTIKIKDGKIEEVGANLPIPMDDVKVFDMKGKHVYPGLILPTSTLGLVEISSV
                     RATQDSREIGDMNPNVRALVAYNTDSKVINTLRSNGVLLANVVPQGSFVAGSSSVVQL
                     DAWNWEDAAYKTDAGIHVYMPSLMPRPSFGRFGGGGGGGPQAPQVDPVKEGLEKIEGL
                     KSFFKEAKAYHAETAHEETNLKFEAVKGLFNKGQKLYVHANTVKQTLVALDFVKEFGF
                     EVVLVGGSESYQVADLLKQNNVSVILQQPHSLPTGQDDDVDQPYKTAAALQKAGVLFA
                     ISDDDPQTRGRNLPFNAGTAAAYGLSKEEALAAISLNAAKIMGVADKTGSIEVGKDAN
                     IVVSEGDLLDMRTSNVTDAFIQGRKIDLTDKHKLLNEKYEQKYELKTKKGF"
     gene            complement(4683..5534)
                     /locus_tag="PLO70_03600"
     CDS             complement(4683..5534)
                     /locus_tag="PLO70_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQZ73570.1"
                     /translation="MYKVTKKKVHGLLHPEIVGDKHWDKIINVFIITLIILNVIAVMI
                     ETIPSFHDDYNEQQFFRYFDLISVIIFTLEYVLRVWSSNQEEKYKHSIWGRLKYMLSP
                     GALIDLMAILPFYLHAIIGFDLRVLRMLRLLRFFRLFRLTAYMKSAHMITNVFKKRIN
                     ELALSLILVIFLIIIASSAIYFSEHLHPENKEKFISIPATIWWAVVTLTTTGYGDMYP
                     MTTAGKIMAGIIMLTGVAFFALPAGIITAGFLEEFRKTRKQKENKCPHCGMLIEDDNH
                     NNHEEDH"
     gene            complement(5641..8973)
                     /gene="secA"
                     /locus_tag="PLO70_03605"
     CDS             complement(5641..8973)
                     /gene="secA"
                     /locus_tag="PLO70_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQZ73571.1"
                     /translation="MLGFISKIFGGSKSEKDVKKIEPYVAKINHFFSSYQSLSNDQLR
                     NKTQEFRQRIKEFLAAIDAEITAKNIAAEELPYNDLLGKDAIYQEVDKLKKDRDKKIE
                     EVLEEILPEAFAVVKETARRFKENTEIIATATQLDRDLSVVKDYITIDGDKSIFKNSW
                     TAAGGQVTWNMVHYDVQLIGGIVLHQGKISEMATGEGKTLVSTLPAYLNALPGEGVHI
                     VTVNDYLARRDQEWNGTIFEWLGITVDCIDKHQPNSEERRKAYLSDITYGTNNEFGFD
                     YLRDNMVHTPEEMVQRKHHYAMVDEVDSVLIDDARTPLIISGPVPKGDDQQFHIHKPR
                     IQQLVQEQEKIVRHCLNEAKKRIAEGDDDPKGGGLYLFRAHRGLPKYGPVIKYLSEPG
                     IRVKMQKAENYYLQDQMRNMHIVDEELLFQIDEKNNSVELTDKGLTLITKTGEDPEFF
                     VLPDIGVKLAEVEKSAVTAEEKLQIKESVLNDYAQKADRIHTVQQLLKAYTLFDKDVE
                     YVIIDGAIKIVDEQTGRIMEGRRYSDGLHQALEAKENVKIEAATQTYATITLQNYFRM
                     YHKLAGMTGTAETEAAELWSIYKLDVVNVPTNLKMIRDDKQDLVYKTKREKYKAVIDE
                     IEALRNAGRPCLVGTTSVEVSELLGRMLQQKKIPHNVLNAKQHAREAQVVAEAGLAGA
                     VTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSTQF
                     YVSLEDDLMRMFGSERIASLMDKLGYKEGDVIQHSMISNSIQRAQKKVEENNFGIRKR
                     LLEYDDVMNMQRNAVYKRRNHALFGERLALDIDTSFSALVEGLVSSFREQEDFEGFRL
                     ACIVNFGLDTKITEDEFKKGDINKLTDQLYNESTERYNRHKEEIQKQAIPVFKNIRLT
                     QGSHIENVVVPFTDGRKGLNVLTNLDKTLSTAGVELTNSLEKTITLAVIDDSWKEHLR
                     AMDDLKQSVQTAYLEQKDPLVIYKMEAYNQFRNMNADVNKDIVSFLCHSSIPIQEENA
                     QLKEGRQQKTDMSRMRANKDDVDNAGQDYAANERDKFEPGGGGEPVVKHEPIKVGPKV
                     GRNDPCPCGSGKKYKQCHGKEA"
     gene            9229..9921
                     /locus_tag="PLO70_03610"
     CDS             9229..9921
                     /locus_tag="PLO70_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73572.1"
                     /translation="MKRLYVLLSIIFIFLLSSCASKDEKAVQNSAVENIYFDYQVLAE
                     EGDDNLTVLIRFREGEEGEAISLSGISSVSLDGEILTADSSKRTGAFYETHKPIANFA
                     GNHTILFKDDEGNEYKEDFLFQPLVLTNYIPDTIKRSDLLFELEGLEPEDYVRVLATD
                     TLFTSKGINRLDTVNNGKLILSLDDLGMLVNGPIQLELIREYENKVKNGTEAGGKIAI
                     NYRLRREFFLKD"
     gene            10045..10926
                     /gene="atpG"
                     /locus_tag="PLO70_03615"
     CDS             10045..10926
                     /gene="atpG"
                     /locus_tag="PLO70_03615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQZ73573.1"
                     /translation="MAGQLKEVRNRIKSVQSTQQITKAMKMVSAAKLRRAQDAIIQMR
                     PYAQKLQEMLSNIVSNSEAGSGMSLAAERPVEKVLLIVITSDRGLAGAFNANIIKLAK
                     ATIVEKYADQNKKGNVTVWNIGKKGYENFIKGNYKADATFKDIFLNLTFENVQKAAKA
                     AMKAFEEKEFDVVEIVYSQFKNAATQRFEVERYLPIPKVEKKAGAVKADFIYDPSKEQ
                     LIAELMPKILNTQLYKAVLDSNASEHGARMTAMDKASENANEMLRALKISYNRARQAA
                     ITTELTEIVSGAAALQG"
     gene            11010..11720
                     /gene="scpB"
                     /locus_tag="PLO70_03620"
     CDS             11010..11720
                     /gene="scpB"
                     /locus_tag="PLO70_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQZ73574.1"
                     /translation="MEITNLIPHIEALIFASDKPLTTLEITELVNNAFGFMEDKISLD
                     QVEAAIEGITEKYASEFYPFEMKQSGGGWQFLTKKEFHKTIAQINGDKFLKRLSAASL
                     ETLAIIAYKQPVTKGEIEAIRGVSSDYAVQKLLEKELIIITGRNEKLPGHPLVYATSK
                     NFMDYFGINSADDLPKIKEVLAEQLVEPTVIKDLNEDGETEVKSDEIEGTDEVEALAV
                     TEGGELIINKEQGEGEEN"
     gene            11799..13016
                     /gene="lysA"
                     /locus_tag="PLO70_03625"
     CDS             11799..13016
                     /gene="lysA"
                     /locus_tag="PLO70_03625"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQZ73575.1"
                     /translation="MSQQLSHEQLVVLAHQFGTPLYVYHAEKIKEQYDKLLTSFKGVD
                     VRFFYAAKALTNINILRYLKSTGCNIDCSSINEAKLAVIAGFAPSDILYTSNNIAFEE
                     IEEAQRMGIHINIDSISNLRKFGQKFGHSYPVGIRLRPNIMAGGNLKISTGHADSKFG
                     VPLEDIEEVLQVIKETGLFIRTLHIHTGSEIKDVEVFAKGIEVLFEMVKYFPELAIID
                     LGGGFKVPYKPGDEGTDIELLGKKVKEEFEWFEKKYNRHLQVWFEPGKYLVSEAGYFI
                     TKVNVLKPAGNAVFAGVDTGLNHFIRPMFYGAYHEIENISNPSGDKRSYNVVGNICET
                     DTFAENRSLPEIREDDYLVFLNAGAYCFEMSSHYNSRFKPAEVMVKDGKPILIRKRDD
                     LEDLLKGQPEIIF"
     gene            complement(13029..13910)
                     /locus_tag="PLO70_03630"
     CDS             complement(13029..13910)
                     /locus_tag="PLO70_03630"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HQZ73576.1"
                     /translation="MFRISVFISVILITSGTVFAQMADGRLKFQQGQVFEINLQTKTT
                     VAQQAMGQSIDFNVDASGIHTYKVTNTTDDNHTLNHTVNRITFSFDGMGQKQSFDSDK
                     PKDMNGQFGKPIKEMLEKKYDMIIDPFGKVLMVMPESFTASANDSRMAVITNMMKEVV
                     DVVQPPGKGKASFFNVIPEKDGGLNLGDAWTQSGETPAEKFESAYSITAITDSTIIVD
                     YVTNSNTVTKAEMMGNETITSMKNKSAGKITLDRVTGIIREKTITTDSNGSTETSFGT
                     LPVTSKTTTIIIVKALQ"
     gene            14079..>15009
                     /locus_tag="PLO70_03635"
     CDS             14079..>15009
                     /locus_tag="PLO70_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQZ73577.1"
                     /translation="MPIRMTDDPVDPNQRDDDGGGGNRPNFPGGGGGGLMSLLPMLLG
                     LFKGKGIIILLVLAAGAYFLLGKGGGCNVAQVAQQLFSKSGYNFDPAEFNKASVYEGL
                     EDDAGKNPLPEAVSLLRFAPERSNQGEQGSCVAWSCAYGAQTILTAAATGADPNTIRF
                     SPAYLYNQIKLEGCQGSYLQRAMEAMQTNGGVPLSQFPYTDQDCNRVPDESAKQAGRQ
                     NVIHGFTRITNGDNINEISVRGIKEHLNKNAPVAIGMMVGQSFMQNMMGQELWEPSGM
                     DASQMGMGGHAMTVIGYDDRKFGGAFQIMNSWGP"
     assembly_gap    15010..15109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15110..15817
                     /locus_tag="PLO70_03640"
     CDS             <15110..15817
                     /locus_tag="PLO70_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HQZ73578.1"
                     /translation="YDDRKFGGAFQIMNSWGPEWGNNGVAWVRYGDFKTYVREAYGID
                     PLSKRSNVANIPLECNIGLVRNEDKQNIALRLAGNNLFETVTPIRVGTRFKMSIENAT
                     ECYIYVFGQEVDGSSFVLFPYLKTGETVSKHSPYCGITGYRLFPKAQSLEADSIGTKD
                     YIAIVVSKDELDYNRINQAISQSRQSTYAGKVNEALQSILIRTARFNSTNDGRIYFKV
                     DANDNKAVAAIVAFDKR"
     gene            15828..16319
                     /locus_tag="PLO70_03645"
     CDS             15828..16319
                     /locus_tag="PLO70_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQZ73579.1"
                     /translation="MNTNAPEFIKMMKHPVKFRMFLFFKLPAAFFAGVRIREVDEKKC
                     VVTVPYKWLSQNPFRSTYFACLSMAAELSTGALAMTQLYKSDPPVSMLVVKVESEYFK
                     KATDKSSFFCEDGELFQKAIEETIATGEPRTVKAKSIGTNKAGEKVAEFYITWSFKSK
                     FKK"
     gene            16394..17797
                     /locus_tag="PLO70_03650"
     CDS             16394..17797
                     /locus_tag="PLO70_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ73580.1"
                     /translation="MKIAFHGAARTVTGSKHLLTLKSGKKILLDCGMFQGLGRETDVL
                     NRSFGFDPLEVDMMILSHAHIDHSGLIPRLVKEGFKGKIFCTHGTRELVTVLLEDSAE
                     IQEDDVKYINKRRAASGQAYLQPLYTVEDALNATKYFEEADYGQWFDVMEGVQAMFTD
                     AGHIIGSTCVHLKINEHGKETRITFSGDIGRYRDAILKSPDTFPQADYIIMESTYGNS
                     LHDTHVTTPDMLLHWIEKACLQKKGKLIMPAFSVGRTQEILYSLNQLEIENRLPDLDY
                     YVDSPLSIQATEIVKNYPNYFNKTIQKIMQSDKDPFSFKGLRYVKTVEQSKLLNFQNG
                     PMVIISASGMGDAGRVKHHISNNIENSHNTILFTGYCEPRSLGGRLLSGDKDVSIFGV
                     MHEVHAEISSIRSMSAHGDYEDMSQWLACQDPKKVKKIFLVHGEYEVQQDFQNRLIRK
                     GFTDVEIPAPHYEIGLT"
     gene            17918..17991
                     /locus_tag="PLO70_03655"
     tRNA            17918..17991
                     /locus_tag="PLO70_03655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17952..17954,aa:Met,seq:cat)
     gene            complement(18087..19154)
                     /locus_tag="PLO70_03660"
     CDS             complement(18087..19154)
                     /locus_tag="PLO70_03660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQZ73581.1"
                     /translation="MKSLYICLLLLNSIHSFARKDDDSTFYRADHSRIQYTGRIDFSN
                     PVKPRFWAPGSYITIRFSGTYCVMEITDEEKWGKSHNYLQVIVNDQPAYRIQTKGRKN
                     KVVLASGLPKGEHTIIICKNTEAEIGYLELIGFTCNKLLSPPKKPKRKMEFIGNSITC
                     GMGNDDSEIPCKTKEWYDQHNAYLAYGPLSARALNAQWHLSSVSGIGLIQSCCNKTTA
                     MPQVFDKINMAENNLTWNFKRYQPDVVTICLGQNDGIQDSAAFCAAYIQFVNRLRNYY
                     PKATIILLTSPMADQPLDSALKKYINVVTTAIKASGEKKIESYFFSRRYNNGCDAHPN
                     AGEHAAMAKELTAYTRQLMQW"
     gene            complement(19197..19856)
                     /locus_tag="PLO70_03665"
     CDS             complement(19197..19856)
                     /locus_tag="PLO70_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQZ73582.1"
                     /translation="MKNALKLILLFLLAFTCSSLLAQPFINEVNYFKKLDSLNAPPAN
                     PVLFIGSSSFTNWKDVKEYFPSYNILNRAFGGSSLPHLVMHAEEVIFRYHPKQIVIYC
                     GENDLAASHKITGDTVFHRFKTLHSLIRSRLPKVPILYVSMKPSPSREKYLPEMSRGN
                     NLIKKFVKKNRKTSFVDVYHKMFTKEGKIMEDIFLSDKLHMNKKGYQIWQPAIEPYLL
                     K"
     gene            complement(19890..20768)
                     /locus_tag="PLO70_03670"
     CDS             complement(19890..20768)
                     /locus_tag="PLO70_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ73583.1"
                     /translation="MNNNILKEITPLTASDCFTIFARTKTEFDFPLHYHEEMELNLII
                     NAKGAKRVVGDHMEEIDDTELVLVGSNLPHVWKTYKCKSNDIQEITIQFHKDLFDDKM
                     LRRNQLHFIRDMFEKAKRGILFSKVTTQLLSGRLRQLRNKQGFDSVLELMSILHDLSI
                     SRNMRILSDASFSNIQQSYNSRRVEKVMEYMNQNFDKTVILADVAKLANMTEVSFSRF
                     FKTHTGISFIDSLLEIRLGHASRLLIDTNQPVAEVAYHCGFNNISNFNRLFKKKKGCT
                     PKQFREDYSYGSRVFI"
     gene            20969..24094
                     /locus_tag="PLO70_03675"
     CDS             20969..24094
                     /locus_tag="PLO70_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73584.1"
                     /translation="MRKYTFLFLSFFVLASVQSLAQSKKLSGAVTDEKGDALAGATVT
                     VRNSMISTITNALGKFSLDVPDDAKALVITFTGMEREEVLIGAKTSFAIMLKPSVTTL
                     SDVVVIGYGTVRKTDVTGAAQRIQREDFIRDNPTNILQAIQGKLAGVNVTQNDGAPGA
                     GLSIRVRGSNSFLGGTEPLYVIDGVPFNNNSSGATPSSIGSDEKQTLNALAFINPSDI
                     ESIDILKDASATAIYGSRGANGVVLITTRKGRSGKDKVELSVNIGLSEVSRWLKMLKP
                     YDYAQYQNLSYTNSNVYDGTSYAMPFTPEELDSLVNDNTNWQDAIFRRSLSQQYSLNV
                     SGGSESGSHSLSLNYINQDGTVINSDYRKIGLSFNLNRNLGKVFKIGTSTSVSNTVTN
                     GVKTGTDKSDAASAGVIRSALTYPPTITAEEEYDGTGEGFFITNPVIYSRDVLNKVTG
                     INIFSSNYAEATLLKHFKFRQNLGFNYGSNKRDQYYPRTVYEGFSVKGWGLKADNLWT
                     SLVSESILSFNKKMEKHNLTVTGASTFERTNGEWNRAEAKTFPNDLLQNENLQSAEQI
                     MPVVGSRFQSTLISFLGRVNYSFDDKYLLSLSYRQDGSSKFGRDNKWAGFPSAGFAWK
                     LHKESFMNRAVAISNLILRLSYGQTGNQGIGSYASLSKLAVYNYPFNGSLQTGLADDV
                     YAGPANASLKWETTKAYNTGVDLGLFKGGLNFHADFYLKETNNLLQYLTTPSSTGFQR
                     QLRNSGSVQNKGAEFSLDGKLISRKDFQWKSAFNIAFNRNKILSLGSDVKEQFASNIS
                     TRDAPFIQTAGQPIGALYGYVEDGYYDNEAEVRSSQVYSSQPMAIIRRMVGEIKYMNL
                     DNDPTSISNSDRTIIGDVNPDYTFGFTNNFSYKNFDVSIFINGVQGNDVVNMNKVFNG
                     NIGTSKNISEKMLEGTWVDGKDNSKATEPKAIRQFWRTLLFSRRFIEDGSFVRVKNIT
                     IGYNVPGKLIKGISSMRLSVGVNNLYTFTNYSGFDPEINSYGDNPALFGVDLGGYPNT
                     RSYNFTLRCSF"
     gene            24122..25741
                     /locus_tag="PLO70_03680"
     CDS             24122..25741
                     /locus_tag="PLO70_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ73585.1"
                     /translation="MRPGKKYLWILSVSILMLSMSSCKKQLEEKPYSFLSPENFYKNE
                     SDAKTAINGVYSALYSWDLYLQPMWNLTILDDDHVSGVDWFLGTSGAGNPQGYWGVDG
                     PWVGCYSIIARASTVLENVTKINENIDPDIKTRILGEAHFLRAWAYFQLVQLYGGVPI
                     RLQSLSVDPNTNVPRSSVKEVYQVIINELKLAETMLFPAGHAKAGEPGRVTQGLAKGM
                     LAKVYLTMASGSVPAANVTVRGGTDNGFYTYAKSVVAGLEGLDSKTYFELARDKALEV
                     INSGQYTLFTSWKTMWSKAAHNTQEHMWELQSLSGTTFVNNIHNYFSALSTFGRGAVW
                     FTNNHYMDYEDPDKRVLDGVAHNYATNTGTRYYYPSWQAALYQTVGGLTYNNNGTTDN
                     RAYVIKYADVTDPTVANSDAYFPMMRYAEMFLIYAEAANEANGSPTTEAYTYLNTIRT
                     RAAASAAPAGLSQEQFRSFVLAERAREFVLEGIRRYDLMRWGIYLQVMNKISAGQNNI
                     SKVRSLRNLLLPIPQSELNSNQAITGNNPGW"
     gene            25774..26832
                     /locus_tag="PLO70_03685"
     CDS             25774..26832
                     /locus_tag="PLO70_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HQZ73586.1"
                     /translation="MKRNLLIHNKILPVMMSMGLILFFGSCKKENTEDRNPPEINAVT
                     DLMNRDIALTSVNYGDWIIIKGKHLSTTYKVEFNTILAEDSLIYADDTTVTVKIPAVL
                     PDPANNPITVTTKYGSATYNFRILQPPPTILSFDPMAGPEGQLVTITGYNFGGVNSVK
                     FGTNEATIVSNTKEEIKVNVPAGVTAEYIFVTTPSGTVRSDKLYGFRYEVFTDALTTG
                     WSFSLSSANVGYNAAIADTVKRGSRSVRIDFRGSFSFLRFVKTAAMSTTGFQGVKFSM
                     YAPANFMDKKVRVYLNNSSTPGSYTINVSKVNQWVEYEIPLINFGNPATLTHIIFNEF
                     SGTAGLPRQFYVDDLGLY"
     gene            26899..27918
                     /locus_tag="PLO70_03690"
     CDS             26899..27918
                     /locus_tag="PLO70_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQZ73587.1"
                     /translation="MIRMVSFFGVLFCCILLNTARGQVCSDKIADSAVKALYKNLFTL
                     QHQFTIFGHQDALAYGVGWKGISKQSDVKSVAGDYPGIYGWDIGHLELQSLNNIDNVS
                     FRKMKQYIREGYQRGGVITISWHLHNPLTGGSSWDTTHGTIASILPGGNKHELFKTWL
                     DRVAAFMNGLKDNNGQFIPVLFRPFHELTGNWFWWCKNVSTAVEFRSLWRFSVNYLRK
                     DKGIHHLLYVYNTANFDNDADFLERYPGDDVVDLVSFDMYQHGKKEDKQEFIDAMRKQ
                     IRILCNVAAEKNKIAALAETGMEAVPDNEWWTGVLWPVIKDFPLSYVLVWRNHGYMQP
                     KKKMH"
     gene            28038..29870
                     /locus_tag="PLO70_03695"
     CDS             28038..29870
                     /locus_tag="PLO70_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HQZ73588.1"
                     /translation="MKLQLLDILIVITYLATMVFIGVYMRKKARENKESYLMGGKKLP
                     WYMLGLSDASDMFDISGTMWMVALCFVYGMKSMWIPWLWPVFNQVFMMMFLSKWLRRS
                     NANTGAEWLATRFGLSGKGVKGSHNVVVIFALIGCFGFLAYGFIGLGKFIEIFVPWEM
                     VSGYIPFNIAPQYAAHFYGIVFTLFAMFYSIIGGLHSIVLGDFIKYIIMTVACVSIAV
                     IAMIHLHQNGNQLRVPDGWLNPFFGWDLDLDWSGIVPDVNKKIEDDGYGLFGIFFMMM
                     LFKGIFASLAGPAPNYDMQKVLSTRSPKEASKMTGFVSIILLPVRYSMVIGLTILALL
                     YYNQLNLSAPGGGTDFEKILPAAVNNFLPVGVLGLVLTGLLGAFMGTFSGTLNAAQAY
                     IVNDVYLKYINPDASNKKIIRTNYIVGVLVVAIGVVLGFFAKDVNSILQWIVGGLYGG
                     YIAANLFKWYWWRFNANGFLWGMASGILGALVFLKIYDGLFLYIWPVLFIISIAGCLI
                     GTYTAPPTEIDTLKSFYKSVRPWGFWKPVHRLVVEEDPSFQPNRRFKLDMFNVVLGVI
                     AQCCLTILPMYIVLWMKLPLLITISILLVIVLILKRTWWDKLED"
     gene            29890..31122
                     /locus_tag="PLO70_03700"
     CDS             29890..31122
                     /locus_tag="PLO70_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HQZ73589.1"
                     /translation="MKNEFNYRLSQLEAMQELLLTKSNEMDGAGNGLYNRYKNPVLTA
                     DHVPLNWRYDFNEKDNPYLIQRFGINAVLNAGAIKWRGKYLLMARVEGYDRKSFFAIA
                     ESLNGIDNFRFWDYPVNMPETAQPDVNVYDIRLVEHEDGWVYGLFCTERRAVGVPESD
                     QSSAVAQCGIARTKDMLNWERLADLVTPSPQQRNVVLHPEFVNGKYAFYTRPQDGFID
                     AGRGGGIGYGLAENIENAVIIEEKLLDRKLYHTVYELKNGLGPAPIKTQYGWVHLAHG
                     VRNTAAGLRYVLYMFMTSLNDITRVIHKPAGYFMAPEGEERIGDVSNVLFSNGWIADE
                     DGNVYIYYASSDTRVHVATSTVDRLVDYVMINPPDGFRSSASVATITTIIDKNRQLNE
                     TLLQPLLAGASKNGKETK"
     gene            31119..32342
                     /locus_tag="PLO70_03705"
     CDS             31119..32342
                     /locus_tag="PLO70_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQZ73590.1"
                     /translation="MIVRLTDSSVKEPLQRYRSQAEAELASVLDWWMQYIPDDEDGFH
                     GEIDRYNKLKADAPRGLVLYSRILWTFSAAYIHTRNREYLFMAERAYRYLIKHFQDTV
                     NGGMYWSVDRHGQKLIDRKQVYGIAFCIYGMSEYYQATFNEQARDKAIELFLLLEQHS
                     RDKKEGGYFEAFAANWQLVPDVRLSEKDANEKKTMNTHLHVLEAYTNLYRIWKNEVLA
                     EALHSLLVVFKKRIIDTATHHQGLFFAENWELRSSVISYGHDIEASWLLYEASEMLGN
                     PEVLHIYKDIVVKMAIAVLPAIDNDGGMWYESETRTGHWVKEKHWWPQAEAMVGFMNA
                     YQLTGDKIYLKYSQRSWDFARQYICDKENGEWHWGVDEYGNMMEKEKAGFWKCPYHNG
                     RACMEISKRISAILQ"
     gene            32728..34407
                     /locus_tag="PLO70_03710"
     CDS             32728..34407
                     /locus_tag="PLO70_03710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQZ73591.1"
                     /translation="MQIKNAFIPVVMGLVMFYSCNEQQDTGGKVILYKNAKIWTGDSS
                     NLSASCIAIRGESILYVGSNSDSIKADSVVDLQGRMIVPGFIDNHTHFLSGGCQLNAV
                     DLRKAKTKNQFIAILNDYITANPGVTWIQGGDWDHEVWGGELPVKEWIDSITGNRPVF
                     LSRYDGHMALANSAALKLAGIDKNTKSPDGGEIIKDTQSGEPGGVLKDDAMGLVYNKI
                     PDPTSQQLDKLLQRATGHAHKNGVTQVHDMGSYGGWIDLETYRRAYTNQTLGIRIYSF
                     VSLSTWQKLADYVKQNGKGDDLLRWGGLKGFVDGSLGSTTAWFYKPYLDAPKSTGLVL
                     NDTNVLRKWILNASAEYLQVAVHAIGDRANDWLLTVFEEAKKINGKQSSRFRIEHAQH
                     LSESAIPKFALLNVIPSMQPYHAIDDGRWAAKRLEDDRLKRTYAFKSLLDANAKLTFG
                     SDWTVAPLTPLEGIYAAVTRKTLDDKNPDGWYPEEKITVEQALRCYTINNAYAGFQEN
                     KLGMLKAGMLADFVVLSDDLFSIPAEKIRDAKVLKTISNGKLVYNSNESKK"
     gene            complement(34537..35424)
                     /locus_tag="PLO70_03715"
     CDS             complement(34537..35424)
                     /locus_tag="PLO70_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HQZ73592.1"
                     /translation="MKRITLVLIFFNFSSLSFAQKSNPDNKIPILFRLDDIGMCHAVN
                     MAAKEVLETGIPVSMSVMVPCPWFSEGVALLKNYSNVSVGIHLTLNSEWKQYRWGPVS
                     GPMAVPTLVDSFGYFFPSRSKLFGNNPKLSEIETELRAQIDKALLAGLKIDYLDYHMG
                     AAMQTPETRAIVEKLAAEYNVAISRYYDEVDVEGGYSAPVSNKLDTMLIKVKNLQPGG
                     TKLFVVHIGLDDPEMSAMEDLNPSGPKEMSKHRYAELKTMVSPQLQELLRDPKYRLIN
                     YRILNEEKGLGTMKRPVVN"
     gene            35842..36612
                     /locus_tag="PLO70_03720"
     CDS             35842..36612
                     /locus_tag="PLO70_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ73593.1"
                     /translation="MVQTRPWRQFLDEAGDISRFTGRFFSQWFRPRYEVAEFFRQCFI
                     IGYKSLPLVGLTGFIMGLVLTMQLRPSLVSYGVESQLPAMVGIAIVREIGPVIAALIF
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKYLVVTRVLASTLMLPILVILADAVS
                     LYGAYLGVNIRGVTSFDLFWQQIFDNLSFGDVVPAFVKSYFFGFAVGIVGCYKGYNSR
                     KGTEGVGRSANSAVVLSSILIFMIDLIAVQITDLLHLN"
     gene            36618..37394
                     /locus_tag="PLO70_03725"
     CDS             36618..37394
                     /locus_tag="PLO70_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ73594.1"
                     /translation="MLATASIVTEKKIITEKEKVIAISHLYKSFGDNKVLVDFNLSLY
                     AGENVVVLGKSGSGKSVLIKCIIGLLEPDNGTIEVFGKNIPDLGHDALDEMRVKVGFL
                     FQSNALYDSMTVRENLEFPLRRHTIKMAQKETDRLVKDALDDVGLAHTIDMMPAELSG
                     GMRKRIALARTLILKPAIILYDEPTTGLDPITGKEIIDLMIEIQKKYNSASLIISHDM
                     NCVRMASDRVIMLIDGKCYAGGTYDELKKLTDPKIKEFFE"
     gene            37400..38383
                     /locus_tag="PLO70_03730"
     CDS             37400..38383
                     /locus_tag="PLO70_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQZ73595.1"
                     /translation="MSKKVISNVKLGFFVIAGLLFLILLLYMIGRNESMFGDYFRLKA
                     RFHNVQGLNSGNNIRFSGIQVGTVKKVKILNDTLIEVDLLIESKMKNYIHKNAIVSIT
                     TDGLMGNRVLDITPSKEPAALVEEDDILVTKKTVDMGDMLQTLAKTNNDVAFIAAELK
                     STMQRVNGSTAIWTLLNDNTLPENLRASLSNARAATAKANSMIHDLNQLVVDIKEGKG
                     SLGALLTDTVFASNLNDAIIKFKTVGEDADKLALELNSLTKNIRNEIDNGKGPLNTLL
                     KDSAMVRNLNQSIENIQKGTAGFNEVMEAMKHNFLLRGYFKKLEKQKAKGM"
     gene            38502..39089
                     /locus_tag="PLO70_03735"
     CDS             38502..39089
                     /locus_tag="PLO70_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HQZ73596.1"
                     /translation="MSIIRLARSSDAEGILAIYAPYIERTSFTFETEVPSPEIFENRI
                     NSYLENWPWLVCEINGSIAGYAYASRYRERAAYQWSVECSVYIHDDFHRRGVGRALYA
                     ALFEILKKQGFRNVYAVINLPNMNSVAFHESCGFNYFATYEKVGYKLGQWKNVGWWRL
                     ILNEFNNEPAAPVCFSQLEKDFLPDVFQKAILLLH"
     gene            complement(39140..41905)
                     /locus_tag="PLO70_03740"
     CDS             complement(39140..41905)
                     /locus_tag="PLO70_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73597.1"
                     /translation="MRKALFLLLTFMCIGVTVIAQQPVTGKVTDANGLPVAGVSVKSK
                     KTGKMALTGADGTFSLTVNSDDEIEISSIGFVKQTIKANVTSPVNITLAQSIEELSQV
                     VLVGSRRAGRAKTETTAPVDVINVSQATSSTARMDLTSVLNYSAPSFNYNKQSGSDGA
                     DHVDLASLRGLGPDQTLVLVNGKRRHQTAFVAMFGTRGRGNSGTDLNALPASAIERVE
                     ILRDGASAQYGSDAIAGVINLITKKSVNQITGSVGVAGYYDPKFNTAFESGLHQDYEH
                     AGKIDGKSLAANVNFGLPFGERGGFINLTFEGVSTSKTFRQALKTDNYISDDDAMYLN
                     IYRRGHGDAGVNTFGTFYNLELPTKGKATLYSYGGHNSKSSDAYAFTRNWSARPDRFP
                     TDAFGDLVFVPEIMRTGSDGEIWYSPHIQTKIKDFSITGGVKSVAWGNWNWDLSNTVG
                     KNNFHFYGDKTFNASLGSAQTFFDDGGFSFLQNTTNLNFSREFAKNFNLGLGAEFRYE
                     NYQIYAGEAASYTNYDLSGNKATGAQGFPGYQPNDEVNAQRKVFGAYADAEFDIIPAW
                     LVNLAVRLENYSDFGFTHNYKFATRVKASKTVNLRGSFSTGFRAPSLQQINFSSTFTT
                     VQAGNIAEVKIAPNYSSLAKAAGIPDLTQEKSLSASVGFTWKATPTLNVTLDGYMTKI
                     KDRVVISGQFDGSDPSLDPGLASQMAALNVSYAQFFANAVNTTNMGVDVVIDYNKKVG
                     DNRFRALFAGNIQNMEIDKINVPDKLSGSAFLQQTFLSDREQKFILASAPNMKGALSF
                     EYGRKKLTLGTRLTYFGKVTLLGYGQDGLGISPTVPLDNGSGDVPDQFDYSGKLVTDI
                     YASHPIFKKLVLHAGVDNLLNAHPDFGVVKGAKDWAYNTETGGAWDAVQMGTAGRRLF
                     VRIAFSF"
     gene            complement(42284..43510)
                     /locus_tag="PLO70_03745"
     CDS             complement(42284..43510)
                     /locus_tag="PLO70_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQZ73598.1"
                     /translation="MLTISQRGNQMPPSPIRKLVPYAEAAKKKGIKVYHLNIGQPDIE
                     TPPAILDAVRHAEIKTLEYSHSAGNESYRRKLVQYYKKVGIDVSHDQILITTGGSEAI
                     MFGFFTCLNPGDEVIIPEPFYANYNGFACAAGVNVVPITSRIETGFALPPIHDFEKVI
                     TAKTKAIIICSPNNPTGYLYSRDEMEALKEICIKNNLYLFSDEAYREFCYDGEYISAM
                     HLQGLDQHVVLMDTISKRYSACGARLGAFVTKNKAVYDVAMKFAQARLSPPGLAQIMG
                     EAAVDLPDSYFDAPKAEYLQRRNLLVQRLNAMQGVFCPNPGGAFYAIAQLPIDDSDKF
                     CQWMLEEFSYNNQTVMLAPATGFYGTAGLGKHEVRLAYVLNLDAINAAMDCLEKALEV
                     YPGKVVTEKITAVAEQ"
     gene            complement(43703..44143)
                     /locus_tag="PLO70_03750"
     CDS             complement(43703..44143)
                     /locus_tag="PLO70_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQZ73599.1"
                     /translation="MKKIFFLATAFVFSLGAFAQTKVDETVKMNTEKHDFGKIKQGVP
                     ITYYFEIKNISDKPIVVENSWASCGCTTPEKIVDPIMPGGVAKLKVEYNAAAVAPFTK
                     DVYIKFAGIEQTKTLQITGEVLSAEAYEIYVKEKGAKTPAPKKG"
     gene            complement(44154..44645)
                     /locus_tag="PLO70_03755"
     CDS             complement(44154..44645)
                     /locus_tag="PLO70_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQZ73600.1"
                     /translation="MKKITLSLLSLTIATGLCAQQPVHSENDGHNHGTVTKPATVSED
                     ALKLKETEYEFGKIPQGKPVYHTFEIVNTGKIALKLDNVQASCGCTTPEWSQDPIAPG
                     ATAKIKVGYNSAAEGPFDKFITITYNTTQTKQIRIKGTVWKAPEGPAPSNSSVELLKK
                     QTF"
     gene            44770..>45802
                     /locus_tag="PLO70_03760"
     CDS             44770..>45802
                     /locus_tag="PLO70_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQZ73601.1"
                     /translation="MENTNPGIITDTDKLSFDRFREEVLNDFRTVCISRETSLLGRKE
                     VLGGKAKFGIFGDGKEVAQVAMAKFFQPGDFRSGYYRDQTFMMAAGLATVEQVFAQLY
                     ADPDLDREPFSAGRQMNCHFATRMVNEEGEWLNLADLKNISSDIAPTAGQMPRALGLA
                     FASRAFRNIEQLKSFKNLSTNGNEVCFATIGDASTSEGHFWETINAKGVLQVPLAVFV
                     WDDGYGISVPKKFQTTKASVSDALSGMQKREGTNGFNIYKVKGWDYAGMCEVFEEGIR
                     LARETHTPVLFHVEEITQPQGHSTSGSHERYKSQERLEWEREWDGIKKLKEWIIANAL
                     AEEHELDEIA"
BASE COUNT        13452 a         8597 c        10274 g        13379 t
ORIGIN      
        1 caaaaggact gtttctgccg ttcatcccaa tagaggcaga tcaacaagtt ttttatgtaa
       61 aatcgttttt cgataacttt cactaaaatc tataaactgt atgagaaaac tgaagagact
      121 ggcgggggca ggattattat tactgcttgc caatgtatca atttcccaac ctacttttcc
      181 ggagaatgga gtggcagatc caaggcatgg tcattttgcc tttactaatg ccaccattgt
      241 aaaagatgcg ggtacaacca ttacaaatgc cacaatggtt gttaaagatg gaaaaattgt
      301 tgcggtagga agcggtttga aaattcctgc aggtgccgtt gaagtggatt gtaaagggaa
      361 gtacatttat ccttctttta ttgatgtgta tgctgattat ggcatacctc agccgcaacg
      421 tacaggtggg ggcggccagt tcaatccttc acaacaacca caattagaaa cagccactaa
      481 gggtgcctat ggttggaacc aggcaattaa aagcgatgcg gaagcataca ggatgtttgc
      541 tgtggatgat gcaaaagcaa aaccgcttcg tgaagcaggt tttggaactg ttcttaccca
      601 tataaaagac ggcattgccc gtggtacggg tgctgttgtt tccctggcct ctgaaaagga
      661 gaacctggtt ataattaaag aaagagcttc cgctcattat tcattcagca agggaacatc
      721 cacacaaagt tatcccggct cgatgatggg aatgatcgcc ttacttcgtc agacatatct
      781 cgatgctcaa tggtataaaa ataaacccgc tgcagagggg cttaacctga gtcttaaggc
      841 ttggaatgaa acacaggacc tgcctcaaat atttgaagcc ggagacaagt ggaacaacct
      901 gcgggccgac cggattggcg atgaatttgg tgtacaatat atcattaagg ccggacagaa
      961 tgaatatcag cgtatcaaag agatgaaagg tactaatgct actttcatat taccacttaa
     1021 ctatccacag gcacaggatg tggaagatcc caacgatgca agatttgttt cgttgcaaga
     1081 tatgaaacac tgggaaatgg ctcctgccaa tgcagctgct tttgaaaagg caggcattcc
     1141 tttttgttta accactgctg atttacgcag tgtaaaccag ttctgggcaa atcttcgcaa
     1201 agcgatggaa tacggattga gtgaaaataa agttatggaa gcgttgacga aaacgccggc
     1261 gatcactctt ggtatatatg ataaagttgg aagccttgaa gctggaaaac ttggaaattt
     1321 cttaatcgcc aacggccccc tttttaatga aaaaacaatc attctgcaaa actgggtgca
     1381 gggaattaaa tacacagtaa aagaagatgc agccagtgtg gccggcactt attctcttac
     1441 tgttacttct ctttccggca aagaaacata tacgctggat gtgaagaatg ccagtgctgt
     1501 aacgatgttt gcaaaagata cattgaattc caaattcagt tttgatggta agttggtgaa
     1561 gatcagttat gccccaatga cccgtcgtca gcggcctgtt atgaaccccg gccagatgcc
     1621 ggattcagta aggagaacgg gcggtggttt cccaggtaga ggtgggtccg gaatggagca
     1681 ggcattacca gttactgcca cccggttaag tggagtaagt aatggaacag aatggaacgg
     1741 aaccggcact gattcattgg ggaatccatt gacatggact gccagcctgg ttaaagcagc
     1801 cgaggcaaaa accgacagtg taaagaagaa agatgtgcct gctttaggaa agatcgttta
     1861 tcctttcgaa ccgttcggat gggatgaaag ccagcaacca aaacaggaaa acattcttat
     1921 ccggaatgca actgtatgga ccaatgaaaa agaaggtgta ttacagaata cagatgtgct
     1981 gataaaaaat ggcaaaatag cagccgttgg taaaaatctt acggaagcta ctgccagggt
     2041 tattgacgga actggcaaac atgttacagc agggatcatt gatgagcatt cacatattgc
     2101 ggctgcctcg atcaatgaag gcggacaatc ggtaacatct gaagtaagga tcgccgacaa
     2161 tttaaacccg gatgatataa atatttatcg ccagcttagt gggggtgtta ctacttcaca
     2221 tatccttcat ggttcagcaa atgtgattgg cgggcaaaca cagcttatca agctgcgttg
     2281 gggtgcaaac gacgatgacc tgaagcttaa aggctggccc ggccagataa aatttgcatt
     2341 gggtgaaaat gtaaagcgtt ccaattttag tattccgggt ggtaacaacc gttatcctga
     2401 taccaggatg ggagtagagc aggtgctgat cgatgccttt accagggcca aagattacca
     2461 gcaagactgg aaagagtata acactgcaaa ggaaaagctg atcaaagaaa agaaatcaac
     2521 cgcttctctg ttagctccac gccgtgatct tgaattggaa gcactggttg aaattttaga
     2581 gaaaaaacgt ttcattacct gccactctta tgtgcaaagt gagatcaacg gggctatgga
     2641 agtagctaat aaaatgggtt acactgttaa tacgtttacc catatactcg agggatataa
     2701 agtagcagat aaaatgaaga agcatggatc atttgcttct acattctccg actggtgggc
     2761 ttataaaatg gaagtggaag atgctattcc ttataatgct gccattatgc aaaaagtcgg
     2821 attaactgtt gcgatcaaca gcgatgatgc tgaaatggcc cgtcgcctta accacgaagc
     2881 ggcgaaaatt gtaaagtatg gtggagttac tgaagaggaa gcgctgaaaa tggtaacatt
     2941 gaatcctgcc ataatgctgc atgtagatga taaagtggga agcctgaaag taggtaaaga
     3001 tggtgatgtg gtgctatgga gtgaccatcc attgagcatc tatgcaaaat cattatatac
     3061 aattgttgac ggcactattt attttgacag gcagaaggac gaacaactgc aaaaagaaat
     3121 agatatggaa aggctccggc tggttagaaa aatgaacgga gaaaaaagaa gtggtgctcc
     3181 aacaattcca gcgcaaccaa gttatcagat catgcatacc tgcagcgatc atgggcatag
     3241 tcatggatta ctggttattg atgcagatga tattacaaac gaataaaaaa acagcaatgc
     3301 gaaaaatatt tatagctatt tcagctttgt tcatgatagc cgccaatgca caggaaacgg
     3361 tgcttcctgc gaaagagcaa aaaggcctgc tatttattaa aaatgccacc atacatgtag
     3421 gcaacggaaa agtgattgag aacggaacaa tcaaaataaa agatggaaaa attgaagaag
     3481 ttggtgctaa ccttcccatt ccgatggatg atgtgaaagt gtttgatatg aaaggtaagc
     3541 atgtttatcc gggattgatt ctgccaacaa gtactcttgg tttggtagag atcagttctg
     3601 tcagggctac acaggattct agagagatag gagacatgaa tccgaatgta agggccctgg
     3661 tagcttataa taccgattca aaagttatca atacacttcg ttccaatggg gttttactgg
     3721 ccaatgtagt accgcaggga agttttgtgg caggttcgtc ctctgtagtt cagctggatg
     3781 catggaattg ggaagatgcc gcttataaaa ctgatgcagg aatacatgtt tatatgcctt
     3841 ctttaatgcc ccgtcccagc tttggaagat ttggtggcgg tggtggcgga ggcccccagg
     3901 caccacaggt tgatccggtg aaagaaggac tggaaaaaat tgaaggattg aagtcattct
     3961 ttaaagaagc aaaagcttat catgctgaaa cagcacatga agaaaccaac ctgaaatttg
     4021 aagcagtaaa agggttgttc aataagggcc agaaactcta tgtgcatgcc aataccgtaa
     4081 agcaaacatt agtagcattg gattttgtga aggagtttgg ttttgaggta gtgctggtag
     4141 gtggaagcga aagctaccag gtagcagatt tgcttaaaca aaataatgta tctgttatct
     4201 tgcaacaacc ccatagtttg ccaacaggac aagatgatga tgtggatcag ccttataaaa
     4261 cagcagcagc tttgcaaaaa gcaggtgtgt tatttgctat ttctgacgat gatccccaga
     4321 cccgtggccg taaccttcct ttcaatgcag gtactgcagc agcttatgga ttatccaaag
     4381 aggaggcatt ggcagccatt tctttgaatg ctgcaaaaat tatgggagtg gctgataaaa
     4441 ctggaagtat cgaagtgggt aaggatgcca atattgtagt tagtgagggt gatctgttag
     4501 acatgcgtac cagtaatgtg acggatgcat ttatccaggg cagaaaaata gaccttactg
     4561 ataaacacaa attgctgaat gaaaagtatg agcaaaaata tgagctgaaa acaaagaagg
     4621 gcttttaatc ttattaaaca gattttatat tttcgggcac cggataaggt gcccgatttt
     4681 ttttaatgat cttcttcgtg attgttatga ttatcatctt cgatcagcat gccgcaatgg
     4741 gggcatttat tttctttttg ttttcgtgtt ttcctgaatt cttccaaaaa gcctgctgta
     4801 ataatacctg ccggtaaggc aaaaaatgct accccggtaa gcataatgat ccctgccatt
     4861 atttttcccg ccgtcgtcat cgggtacata tctccatagc cggttgtcgt caatgtaaca
     4921 acagcccacc agatggtagc ggggatactg atgaattttt ccttgttctc gggatgcaga
     4981 tgctctgaaa aataaatagc gctggaggca atgatgatca ggaagatgac cagtataaga
     5041 cttaatgcca gctcatttat tctttttttg aaaacatttg tgatcatgtg tgctgatttc
     5101 atgtaggctg ttagccggaa cagccgaaag aacctgagaa ggcgtaacat ccgcagtact
     5161 cttaaatcaa atcctatgat cgcatgcagg taaaagggta aaatagccat caagtcaatt
     5221 aaagcacccg gtgacaacat atacttcagt cgtccccata tactatgttt atacttttct
     5281 tcctgattac tgctccatac ccgcaggaca tattctaacg taaatatgat tacggaaata
     5341 aggtcgaaat agcgaaagaa ttgctgttca ttatagtcat catgaaagga aggtattgtt
     5401 tcaatcatta ccgctattac attcaggata ataagtgtga tgataaatac attaatgatc
     5461 ttatcccaat gcttatcacc tacaatttca ggatgcagta atccatgcac tttctttttt
     5521 gtaaccttgt acatccaaca gcagttttga tccttctaaa gataaaataa aatcccccgg
     5581 ttaattcaac cgggggattt ttattaatga attaaaatat tgtcagacct gagattggga
     5641 ttatgcttct ttcccatgac attgcttgta ctttttgcca cttccgcaag ggcaggggtc
     5701 attgcgtccg acttttggtc cgacttttat cggctcatgt tttacaacag gttctccgcc
     5761 accaccgggt tcaaatttat ccctttcatt agcagcataa tcctgtccag cattatcaac
     5821 atcgtcttta ttggctctca tcctgctcat gtctgtcttt tgctgacgtc cttctttcaa
     5881 ctgcgcattt tcctcctgga taggaataga agaatgacaa aggaatgaaa caatatcctt
     5941 gttcacatca gcattcatgt tcctgaactg gttatacgct tccatcttat aaataaccaa
     6001 cgggtctttc tgctccaggt aagcagtctg cacactttgt ttcaggtcat ccatggctcg
     6061 caggtgttct ttccaagagt catcgatcac tgccagggta atggtttttt ccaaagaatt
     6121 agtcagttca acaccggctg tgctgagtgt cttatccaga ttggttaaca cattcaggcc
     6181 tttacgacca tctgtgaagg ggaccactac attttcaata tgactgcctt gtgtcagcct
     6241 gatatttttg aagacaggga tcgcctgctt ctgaatctct tctttgtggc gattatatct
     6301 ttctgtagac tcattataaa gctgatcggt cagtttattt atatctcctt ttttgaattc
     6361 gtcttctgta atttttgtat ccagtccaaa gttgacaata caagccagtc tgaaaccttc
     6421 aaaatcttcc tgttcacgga aagagcttac caggccttcc acaagcgccg agaatgaagt
     6481 gtcaatatcc aatgcaagcc tttcaccaaa gagggcatga ttacgtcttt tataaacagc
     6541 attacgttgc atgttcatta cgtcatcata ttccaacaat cgtttacgga ttccaaagtt
     6601 gttttcttcc actttctttt gtgcccgttg gatgctgtta ctgatcatgc tatgctgtat
     6661 cacatctcct tctttgtagc ccagcttatc catgagtgag gcgatacgtt cactgccgaa
     6721 catacgcatc aggtcatctt cgagcgatac atagaactga gtagaacccg gatcaccctg
     6781 gcggccggca cggccacgca actgtctgtc tacacgacgg ctttcatgtc tttctgtacc
     6841 aataatggcc agccctccgg cttctttcac accgggaccg agtttgatgt ccgtaccacg
     6901 acctgccatg tttgtcgcaa tcgtaacagc accggctaat ccagcttcag caacaacctg
     6961 tgcttccctg gcatgttgtt ttgcgttcaa aacattatgg ggaattttct tttgctgtaa
     7021 cattcttccg agcagttcac ttacttccac tgaagtagtt cccaccaaac aaggccggcc
     7081 cgcattacgt aaagcttcta tctcgtcaat aaccgcttta tatttttctc ttttagtttt
     7141 ataaaccagg tcctgtttat catccctgat cattttgaga ttggtaggaa cattaaccac
     7201 atctaattta taaatactcc ataattctgc tgcttctgtt tctgctgtac cggtcatgcc
     7261 ggccagctta tggtacatac ggaaataatt ttgtaaagta atagtggcat aggtttgtgt
     7321 agcagcttca atctttacat tttcctttgc ttccaatgcc tggtgcaaac catcgctgta
     7381 gcgccggcct tccatgatac ggccggtttg ttcatccaca atcttgattg ctccgtctat
     7441 tattacatat tccacatctt tatcaaaaag tgtatatgct tttagtaatt gctgtacagt
     7501 gtggatacga tcagcttttt gagcatagtc attcagcact gattctttga tttgtaattt
     7561 ctcttccgcc gttacagcac ttttttctac ctcggccagt ttaacgccga tatcaggtaa
     7621 tacaaagaat tcaggatctt ccccggtttt tgtgatcagg gtgagacctt tatctgttag
     7681 ctctaccgaa ttattcttct catctatctg aaaaagcagt tcttcatcaa caatatgcat
     7741 attccgcatc tgatcctgca ggtaataatt ttctgctttc tgcattttta ccctgattcc
     7801 cggttcgctc aaatacttaa tcacgggacc gtatttcggt aaaccacgat gtgccctgaa
     7861 caggtaaagc ccgccacctt tcggatcatc atcaccttcg gcaatacgtt tctttgcttc
     7921 attcaggcag tgacgtacga tcttttcctg ttcctgcacc agctgctgaa ttcttggttt
     7981 atgaatatgg aattgctgat catccccttt tggcaccggg ccagaaatga tcaacggcgt
     8041 tcttgcatcg tcaatcaata cggagtccac ctcatccacc atagcataat gatgtttacg
     8101 ctgaaccatt tcctccggcg tatgcaccat gttatccctg agataatcga aaccaaattc
     8161 attattagtg ccataagtaa tatcagacag gtatgctttt cttctttcct cgctgttggg
     8221 ctgatgttta tcaatacaat caactgtgat acccagccat tcgaagatgg tcccgttcca
     8281 ttcctggtca cgacgggcca ggtaatcatt taccgttaca atgtgaacac cttcacccgg
     8341 taatgcatta agataagctg gcagggtaga aacaagggtt ttgccctcac cggtagccat
     8401 ttcagatatc tttccctggt gcaatacaat tccaccaatc aactgaacat catagtgaac
     8461 catgttccag gtaacctgtc cgcctgcagc tgtccagctg tttttaaata ttgatttatc
     8521 gccatcaata gtgatataat cctttacaac actcaaatcc ctgtccagct gcgtggcagt
     8581 cgcaatgatt tctgtatttt ctttaaaacg gcgggctgtc tcttttacta cggcaaaagc
     8641 ctccggaagg atttcttcca gtacttcttc aattttttta tcccggtctt ttttcaactt
     8701 atctacttcc tggtaaatag catctttacc caacaggtca ttgtaaggaa gttcttcagc
     8761 ggctatattt tttgcggtta tctctgcatc gattgctgcc aggaattctt tgatacgctg
     8821 acgaaattcc tgcgttttat tgcggagctg atcattactt agtgattgat aagaagaaaa
     8881 aaagtggttg attttggcaa cataaggttc aatcttcttt acatcttttt cagacttgct
     8941 gccgccaaaa atttttgaaa tgaaaccaag catggctatt ttgattagtt attatgtaat
     9001 aaggtatcag gatgtcaaaa acggtgctga aaaaaatata agccattttg acagcgattg
     9061 ccccggttta aaggggccgg caaagataag ccttagtttg gagtttgttt acagctgttt
     9121 tatgaatact gtcaaccatt taagggtctg aaaaagaaac cctcattaac agatattatt
     9181 cattcgttaa tgtatcttgg tgggctaaaa attcttcaaa tttatccgat gaaaaggctg
     9241 tacgttttgc tttctataat attcattttt cttctcagct catgcgccag taaagatgaa
     9301 aaagctgtgc aaaattcggc ggtggagaat atttactttg attaccaggt gctggcagaa
     9361 gaaggagatg ataacctgac agtattgatc aggttcagag aaggtgaaga gggtgaagct
     9421 atttcgcttt cgggaatttc atccgtttcg ctggacgggg aaatactgac tgctgatagt
     9481 tcaaagcgaa cgggagcttt ttatgaaaca cataagccaa tagcaaactt tgctggtaac
     9541 catactatcc tgttcaaaga tgatgaggga aatgagtaca aagaagattt tctatttcaa
     9601 ccattggtgc taaccaatta tataccagat acaattaaaa gaagtgatct tttatttgag
     9661 ttggaagggc ttgaaccgga ggattatgtg agagttctgg cgactgatac cctctttaca
     9721 agcaagggta tcaaccggct cgatactgtg aataatggca aattaatcct ctcattggat
     9781 gatctgggaa tgttggtaaa cggccctatt cagctggagc ttatcaggga gtatgagaac
     9841 aaggtaaaaa atggtacaga agcggggggt aaaatagcta ttaattacag gctcaggaga
     9901 gagttttttt taaaagactg agctgatcca gagagattta cacaactaaa taacaacagc
     9961 gttacccggg gcaggaatat tttaatatag cgatggaatt taagccctca gaccatacct
    10021 ttgcggccaa tttaagcaag atttatggca ggtcagttaa aagaggttag aaaccgcatc
    10081 aaatcagttc aaagtacaca gcagatcacc aaagctatga aaatggtgag tgctgcaaaa
    10141 ctgcgccgtg cacaggatgc tattattcaa atgcgtccat atgcacaaaa gttgcaggag
    10201 atgctaagca atattgtaag taacagtgag gcaggatcgg gtatgtcgct ggcagcggaa
    10261 cgtccggtag aaaaagtttt gctgattgtg attaccagtg accgtggcct ggctggagct
    10321 tttaatgcca atatcataaa actggcaaag gctactatcg ttgagaaata tgctgatcag
    10381 aacaagaaag gaaatgtaac agtatggaat attggtaaaa aaggatacga gaattttata
    10441 aaagggaact ataaggcaga tgctactttc aaagatattt ttctgaatct tacttttgaa
    10501 aatgttcaga aagctgctaa agccgcgatg aaggcatttg aagaaaagga atttgatgtg
    10561 gtggaaatcg tatacagcca gtttaaaaat gcagcaacac aacgctttga ggtggaaaga
    10621 tatttgccaa tcccgaaagt ggagaaaaag gccggggctg ttaaagccga ttttatttac
    10681 gatccttcca aagagcaatt gattgctgag ctgatgccaa agatcctcaa tacacaatta
    10741 tataaagcag tactggattc caatgcatca gagcatggtg ccaggatgac ggcaatggat
    10801 aaagccagcg aaaatgccaa cgaaatgctc agggctttga agatttcata taaccgtgca
    10861 agacaagctg ctattacaac agagttgacg gagattgtaa gcggtgctgc tgcgttgcag
    10921 ggctaaaatg tgagaaacta atttttgaat tgttaacttt ttcatgaagc ttgcggctat
    10981 tgttgcaagc tttttaattt cgaacagata tggagattac gaatctgata ccacatattg
    11041 aagcattgat ttttgccagt gataagccat tgacaaccct ggaaataact gaactggtga
    11101 ataatgcctt tggcttcatg gaagataaaa tttcactaga ccaggtagaa gctgccattg
    11161 aagggatcac agaaaaatat gcttctgaat tttatccttt tgaaatgaag caaagcggag
    11221 gtggctggca atttctgact aaaaaggaat tccataaaac aattgcacag atcaatggtg
    11281 ataaatttct gaaacgcctt tcagccgcat cgctggaaac gctggctatt atcgcttaca
    11341 agcaaccggt cacaaaaggc gagattgagg ctatccgggg tgtaagttct gattatgccg
    11401 tacagaaatt actggaaaaa gagttaatta tcattacagg ccggaatgaa aaattacccg
    11461 gtcatccatt ggtatatgcc acatccaaaa actttatgga ttacttcggt attaattcag
    11521 ctgatgattt gccaaagatc aaggaagtac tggccgagca attagtagaa cccaccgtta
    11581 ttaaggattt gaatgaggat ggagaaacgg aagtgaagtc agacgagatt gaaggaactg
    11641 atgaggtgga agcattagcc gttactgaag gaggtgagct gatcatcaat aaagagcagg
    11701 gtgaagggga ggagaattaa ttaaggattt gaataatatt tatataggaa agtggttcag
    11761 cgttaactga accacttctt ttttatcttc gccaacctat gtcacagcaa ctttctcacg
    11821 aacaactggt agtactggct catcaatttg gtacaccttt gtatgtctat catgccgaaa
    11881 agatcaaaga gcagtatgat aaattgctga cctcttttaa aggtgttgat gtacgttttt
    11941 tctatgctgc aaaagcactt actaatatca atatactccg ttatttaaaa agtacaggtt
    12001 gtaatatcga ctgcagttct attaatgaag caaagctggc agttattgca ggttttgcgc
    12061 catctgatat tctgtacact tccaataata ttgcattcga agaaatagaa gaagcgcaac
    12121 ggatgggtat tcatattaat attgatagca tttctaacct gagaaagttt ggtcagaaat
    12181 tcgggcatag ttatcctgtc ggtatacggc tacggcctaa tattatggcc ggaggaaatc
    12241 ttaaaatatc gacaggccat gccgatagta aatttggtgt gccgctggag gatatcgaag
    12301 aagtattaca ggtgataaag gaaacaggcc tgtttatccg caccttgcac atacatacag
    12361 gcagtgagat aaaagatgtg gaagtatttg caaaagggat tgaagtgctg tttgaaatgg
    12421 ttaagtattt tccggagtta gcgattattg acctgggagg tggatttaaa gtgccttata
    12481 aaccgggtga tgaaggaacc gacattgagt tattagggaa aaaagtaaaa gaagaatttg
    12541 agtggtttga aaaaaaatac aaccgccatc tccaggtttg gtttgaaccc ggaaagtatt
    12601 tagtaagtga agcaggatat tttatcacga aggtgaatgt gctgaaaccc gccggtaatg
    12661 cagtttttgc cggggtggat acagggctta atcattttat ccgccctatg ttttatgggg
    12721 cttatcacga aattgaaaat atttcaaatc cttcaggtga taaaaggagt tataatgtag
    12781 ttggaaatat ttgtgaaaca gacacatttg ccgagaaccg ctctttacct gaaataaggg
    12841 aagatgatta tctagtcttc ctcaatgccg gtgcctattg ctttgaaatg tcctctcact
    12901 ataattcaag gttcaaaccg gcagaggtga tggtaaaaga cggaaagccc attcttatac
    12961 gtaagagaga tgacctggaa gacctgctaa aaggccagcc tgagatcatt ttctgactcc
    13021 cggattctct actgaagagc ttttacaatt attattgttg ttgttttgga ggtaactggt
    13081 aatgttccaa atgaagtttc agtgctgccg tttgaatcgg tagtaattgt tttttccctg
    13141 ataattccgg ttaccctgtc aagagtgatc ttgccggcag atttattttt catgctggtg
    13201 atagtctcat tacccatcat ttctgctttg gtaacagtat tggagttagt gacgtaatcc
    13261 actatgatgg tagaatcggt aatcgctgta atagaataag ctgattcaaa tttctctgca
    13321 ggtgtttctc cggattgagt ccatgcatca cccaaattta gtcctccatc tttttccggg
    13381 attacattaa aaaaactggc tttgcctttg ccgggcggct gtaccacatc aactacttct
    13441 ttcatcatat tcgtaataac agccatcctg ctgtcatttg ctgaagccgt aaaactttca
    13501 ggcattacca tgagtacttt accaaatggg tcgatgatca tatcatattt tttctccagc
    13561 atttctttga taggtttacc aaactgccca ttcatatcct tgggtttatc cgaatcaaag
    13621 gattgctttt gccccatgcc gtcaaaggaa aaagttattc tgttaacagt atgattaaga
    13681 gtgtggttat catctgttgt attggttact ttgtaggtat gaataccact ggcatcaaca
    13741 ttaaaatcga tagactgacc cattgcctgc tgggcaactg tagtttttgt ctgtaaatta
    13801 atttcaaaaa cttgtccctg ttgaaacttt aatcgtccat cagccatttg cgcaaagaca
    13861 gtaccggaag tgatcagtat tactgaaata aacacactaa ttctaaacat aggaagggta
    13921 tattttttta aaatgattat ccaacaaatt tgacgattct tcgccatatt cgttgcactg
    13981 cgctggaaaa ataaatgtta ttacaggctt gcttggtaca acgtatttta tcttaacttt
    14041 ctattttaaa agacaattta atttaaaaac tattcactat gcctataaga atgacggatg
    14101 accctgtaga tcctaatcag cgggatgatg atggaggtgg tggaaaccgg ccgaattttc
    14161 ccggtggtgg cgggggtggc ctgatgtcgc tgttaccaat gttgttaggc ctgtttaaag
    14221 ggaaaggaat tataatccta ttagttctgg ctgccggcgc ttatttttta ttaggaaagg
    14281 gaggcggttg taatgtagcc caggtagcac agcaactatt ttcaaaaagc gggtataatt
    14341 ttgatccagc tgagtttaat aaggcttcag tatacgaagg attagaggat gatgcgggaa
    14401 agaatccttt accggaggca gtatcgctgt tgcggtttgc tccggaaagg tccaaccagg
    14461 gtgaacaggg tagttgtgtg gcctggagtt gtgcgtacgg tgctcaaacc attttaacag
    14521 cggcggcaac aggagcagat cctaatacta ttcgatttag cccggcttat ttatacaacc
    14581 agattaaact ggagggttgc cagggttctt atctgcaacg ggcaatggaa gctatgcaaa
    14641 ccaatggagg tgtgccactg agccagttcc cttatactga ccaggattgc aacagggttc
    14701 ccgatgaatc agcaaagcaa gccggaaggc agaatgtgat tcacggcttt acccgtatca
    14761 ccaatggtga taatatcaat gagatcagtg tcagaggcat caaagaacac ctgaacaaaa
    14821 atgcgccggt agcgattgga atgatggtag gccaaagttt tatgcaaaac atgatggggc
    14881 aggaactgtg ggaacccagt ggtatggacg cttcgcaaat gggaatgggt ggccatgcga
    14941 tgacagttat tgggtatgat gacaggaaat ttggtggtgc tttccagatc atgaacagct
    15001 ggggacctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atgatgacag
    15121 gaaatttggt ggtgctttcc agatcatgaa cagctgggga cctgaatggg gcaacaatgg
    15181 cgtagcctgg gtgagatatg gcgattttaa aacctatgtg agagaagctt atggtatcga
    15241 cccattatcc aaaagaagta atgtggccaa tattccgctg gagtgcaaca ttggactggt
    15301 gagaaatgag gataagcaaa atattgcact tcggttggcc ggtaataatt tatttgaaac
    15361 ggtgacaccg atcagagtcg gcacccgttt caaaatgtca attgagaatg ccacagagtg
    15421 ttatatctat gtgtttggcc aggaagttga tggttccagt tttgttttat ttccttacct
    15481 gaaaaccggt gaaacagttt ctaagcactc cccttattgc ggaattaccg gttatcgttt
    15541 attcccaaaa gcgcagtcgc tggaagcgga tagtatcggt acaaaagatt atatagcaat
    15601 agtggtgagt aaagatgaac tggattataa ccgtatcaac caggccatca gccagagtcg
    15661 ccaatctacc tatgcaggaa aagtaaatga agcattacaa agtatattga tccgtactgc
    15721 gagattcaat agcactaatg atggcaggat ttatttcaaa gtagatgcaa atgataataa
    15781 ggcggtagca gctattgtgg catttgataa aagataatcc tggttagatg aatactaatg
    15841 cacctgagtt tataaaaatg atgaaacatc ctgttaagtt caggatgttt ctgtttttca
    15901 aattgccggc agcctttttt gcaggtgtaa gaataaggga ggtagatgaa aagaaatgtg
    15961 tagtgacagt accctataag tggctgtcac aaaatccttt ccgttccact tactttgcct
    16021 gcctgagtat ggccgctgaa ttgagtactg gggcattagc tatgacacaa ttatacaaaa
    16081 gcgatccgcc tgtttctatg ctggtcgtaa aagtagagtc ggagtatttt aaaaaagcaa
    16141 ctgataaatc aagttttttt tgtgaagatg gtgagctatt tcaaaaagca attgaagaaa
    16201 ccatcgcaac cggagagcca agaacagtta aagcaaaatc aattggaacg aataaggcag
    16261 gtgagaaagt agcggaattt tatattacct ggtcctttaa atcaaaattc aagaagtaaa
    16321 aatattttct aacttattta aaatctcccg ggaagaacga acaggttctc tcatcagggg
    16381 cggaggagta aaaatgaaaa ttgcatttca cggcgctgcc agaactgtta cgggttcaaa
    16441 acatttactg actttaaaaa gtggtaaaaa gatattattg gattgtggta tgttccaggg
    16501 attagggaga gaaacagatg tactgaaccg tagttttggt tttgatcccc tggaagtgga
    16561 tatgatgata ctttcccatg ctcatattga ccatagcggg ctgatacccc ggctggtgaa
    16621 ggaaggcttt aaaggaaaaa tattctgcac tcatggtacc cgggaattgg taacagtgtt
    16681 actggaagat tcggcagaga tacaggaaga tgatgtaaaa tatattaata agcgaagagc
    16741 cgcttcgggt caggcttatc tgcaaccact atacaccgtt gaggatgcac tgaatgcaac
    16801 aaaatatttt gaagaagcag attatggcca atggttcgat gtcatggagg gggtacaggc
    16861 aatgtttacc gatgcaggcc atatcatagg aagtacctgt gtgcacctga aaataaatga
    16921 acatggaaaa gagacccgga taacgttcag tggtgatata gggcgatacc gggatgccat
    16981 tcttaaatca ccggatactt tcccgcaggc tgactacata attatggaat caacctatgg
    17041 caacagcctg catgataccc atgttacaac cccggatatg ttgttgcact ggattgaaaa
    17101 agcctgcttg cagaaaaaag ggaaactcat catgccggca ttcagcgtag gccgtaccca
    17161 ggaaatattg tattccctga atcagctgga aatcgaaaac cgcttgccgg atcttgacta
    17221 ttatgtggat agtccgctga gcatccaggc aacagagatt gtaaaaaatt accccaatta
    17281 ttttaataag actattcaga agataatgca gtcagacaag gacccattct cttttaaagg
    17341 attgcgatat gttaaaacgg tggagcaatc caaattgctg aattttcaga atggccctat
    17401 ggttatcatt tctgccagcg ggatgggtga tgccggccgg gtaaaacatc atatcagtaa
    17461 taatattgaa aacagccaca atacaatcct gtttacagga tattgtgagc ctcgttcatt
    17521 agggggcaga ttactttccg gagataaaga tgtaagtatt ttcggtgtca tgcatgaagt
    17581 gcatgctgag atcagttcta tccggagtat gagtgctcat ggagattatg aggatatgag
    17641 ccagtggctg gcttgccagg accctaaaaa ggttaaaaaa atatttttag tacatgggga
    17701 gtatgaggtg cagcaggatt ttcaaaacag gcttatccgg aaggggttta cggatgtaga
    17761 gataccggca cctcattatg aaatagggct aacctgacaa gatgtatatt ctaaatttat
    17821 tactgctttt ccatgctgga gatataaatg caggcagaaa atccgatcaa atttcatttc
    17881 tcccatttag gcaaaatagc tatttttgcc gcccgttgcc ggggtagctc agctggttag
    17941 agcatcggat tcataacccg aaggtcggca gttcaagtct gctctccggt actttgtaaa
    18001 aagcctgaaa ctcattgttt tgggcttttt cgcttaagca gcatgaccac gccagttgtt
    18061 tcaattttaa ttattgattt gaagttttac cattgcatca attgcctggt atatgccgtt
    18121 aattctttcg ccatagctgc atgttcccct gcattggggt gggcatcgca gccattgttg
    18181 taacgccggc tgaaaaaata gctttcaatt tttttttcac cacttgcctt tatcgctgtt
    18241 gtaacgacat tgatatattt tttcaaagca ctgtccagtg gctgatcagc catcgggctc
    18301 gtcaataata taatggtggc tttcgggtaa taattcctga gccggtttac gaattgaata
    18361 taggcagcac agaaggccgc tgagtcctga atgccatcat tttgcccgag gcaaatggtt
    18421 accacatctg gctgatatct tttgaaattc caggtaagat tgttttcggc catatttatt
    18481 ttatcaaaaa cttgcggcat agccgtggtt ttattacagc aactttgaat aagaccaata
    18541 ccggaaacag aggaaaggtg ccactgggca ttcaatgctc tggctgatag cggtccataa
    18601 gccaaatatg cattatgctg gtcataccat tcttttgttt tacaaggaat ttctgaatca
    18661 tcattgccca tcccgcaggt aatggagttg ccgataaact ccatttttct tttaggtttt
    18721 ttgggagggg acaataattt gttgcaggta aaaccaatca attcgagata acctatttct
    18781 gcttcagtgt tcttgcatat aataatggtg tgctctcctt ttggtaaccc gctggccagt
    18841 acaactttat ttttacggcc ttttgtctga atgcgatagg ccggctgatc attaacaatc
    18901 acctgtaaat aattgtgcga tttgccccat ttttcttcat ccgttatttc catcacacag
    18961 taagtgccgc taaaacggat ggtgatatat gagcctggtg cccagaatct gggtttaaca
    19021 ggattggaga aatcaatcct ccctgtatat tgtatgcgag aatggtcagc ccggtaaaag
    19081 gtactgtcgt catctttgcg ggcaaaagaa tgaatgctgt tgagcaatag taaacaaata
    19141 tatagtgatt tcatcccggt gttgtaaaaa tggtataaaa tggaatttga cttaatttat
    19201 ttcagcagat aaggttcaat ggcgggttgc catatttgat agcctttttt attcatgtgg
    19261 agtttatctg acaagaagat gtcttccata atctttcctt cttttgtaaa cattttatga
    19321 tacacatcta caaaagaggt ttttctattt ttcttcacaa attttttgat aagattgttt
    19381 cctctgctca tttccggtaa atatttttca cggctggggc ttggtttcat agacacatat
    19441 aatatgggaa ctttaggcag cctgctacgg ataagagaat gcaatgtttt gaaccgatga
    19501 aaaacagtat cgccggtgat tttgtgtgag gcagccaggt cattttctcc acaatagata
    19561 acaatttgtt ttggatgata cctgaagatc acttcttcag catgcataac gaggtggggc
    19621 agggaagagc ctccaaatgc ccgattaaga atattgtagg aagggaaata ttcttttacg
    19681 tctttccagt ttgtaaagga ggaactgccg ataaaaagaa cgggattcgc tggcggcgca
    19741 ttcaatgaat ccagtttttt aaaatagttg acttcgttga taaagggctg ggccagcaaa
    19801 ctactgcagg taaaagctaa gagaaaaagt aagataagtt tgagtgcatt tttcatttgt
    19861 ataatttatt attgaccaga ctatttgatt tatataaaca cccggcttcc gtaactatag
    19921 tcttcccgga attgttttgg ggtacaacct tttttctttt taaaaaggcg gttgaaattg
    19981 gaaatattat taaagccaca atgataagct acttcagcta cgggctggtt ggtatcaatc
    20041 agcaggcggg atgcatggcc cagtcttatc tccagcaggc tatcaataaa gcttatgccg
    20101 gtatgtgttt taaaaaaacg gctgaaagaa acttctgtca tgtttgccag ttttgctaca
    20161 tcggctaata tgacggtttt gtcaaagttt tggttcatat attccattac tttctctacc
    20221 cggcggctgt tatagctttg ttgtatatta ctaaaactgg catccgatag tatccgcata
    20281 ttcctggaaa tggacaagtc atgcagaatg gacattaact caaggactga atcaaagccc
    20341 tgcttattgc gtaattgccg aagacggcca cttaaaagtt gcgtggtgac tttggaaaag
    20401 agaatgcctc tttttgcctt ttcaaacata tcccggataa agtgcagttg gtttcggcgg
    20461 agcattttat catcaaatag gtctttgtga aactggatag ttatttcctg gatgtcattg
    20521 cttttacatt tatacgtttt ccagacatgc gggagattgg atccaactaa tacaagttcg
    20581 gtatcatcaa tttcttccat atgatctccc actactcgtt tagctccctt tgcattgata
    20641 ataaggttta attccatttc ttcatgatag tgcaacggaa aatcgaattc cgttttagtt
    20701 cgggcaaaaa tggtaaaaca atcacttgct gtcaaagggg taatctcttt caggatatta
    20761 ttattcataa taaggttaaa ataatatcag tttaatggtg ggtataaaag tattgatatt
    20821 tccttaaatt agaaatattt catagacgat aataaacttt taaattgaat gaagcctgcc
    20881 ggattcaatt aaaatacaat aattggataa aatagtatta tttcgaaggt agcataaaaa
    20941 cctacttttg gtcaaacatt gttgctatat gagaaaatat acatttttgt tcctgtcatt
    21001 ttttgtactt gccagtgtac aaagcctggc gcagagcaag aaattatcag gagccgttac
    21061 agatgaaaaa ggagatgcac ttgccggtgc aactgtcact gtaaggaaca gcatgatatc
    21121 cactataacg aatgccctgg gtaagttttc tttagacgtt cctgacgatg ccaaagccct
    21181 tgttatcacg tttacaggga tggagagaga agaggtacta attggggcga agacttcttt
    21241 tgctataatg ttaaaaccat cggttactac attatcagat gtggtggtca tcggctatgg
    21301 cacagtaagg aagacagatg tgacgggtgc tgcccaacgc atccagcggg aagattttat
    21361 ccgggacaac cccactaata tattgcaggc aatacagggc aagctggcgg gagtaaatgt
    21421 aacacagaat gatggcgctc ctggtgcagg ccttagtatc cgtgtacggg gatctaattc
    21481 tttcctgggt ggtacggaac cgttatatgt aattgatggt gttcctttca ataacaacag
    21541 cagtggcgca acaccttctt caattggctc tgatgagaag cagacattga atgcactggc
    21601 ttttatcaat cccagtgata ttgaatccat cgatatttta aaagatgctt ctgcaacggc
    21661 catttatggc tcaagaggcg caaacggtgt agtgctgatc actacccgca aggggcgcag
    21721 tggaaaggat aaagtggagc tttctgtgaa tattggtttg tcagaagtgt ctagatggtt
    21781 aaaaatgctg aaaccttatg attatgcaca gtaccaaaac ctttcctata ccaactccaa
    21841 cgtttatgat ggtacttctt atgcaatgcc ttttactcct gaggagctgg attcgctggt
    21901 gaacgataat actaactggc aggatgctat tttcagaaga agtttatcac aacaatatag
    21961 tttgaatgtg tcgggaggat ctgaatctgg cagtcatagt ttatcattga actatattaa
    22021 ccaggatggg actgttatca actcagatta caggaagatt ggtcttagtt ttaaccttaa
    22081 ccgtaacctg ggaaaggttt tcaaaattgg aaccagcact tccgtatcca atacagtaac
    22141 aaacggagtg aaaacgggta cggataaatc agatgcagcg agtgccggtg ttatccgttc
    22201 tgcgctcact tatccgccaa caataactgc cgaagaagag tatgatggaa ccggcgaagg
    22261 cttcttcatt accaacccgg tcatttattc gagggatgta ctgaataagg taaccggtat
    22321 aaatattttt tcatccaatt atgcagaagc tactttgctt aagcatttta aattcaggca
    22381 gaatcttgga ttcaactatg gctctaataa aagagaccaa tattatcccc gcactgtgta
    22441 tgaaggtttc agtgtaaaag gctggggatt aaaggctgat aatttatgga ccagtctggt
    22501 atctgaaagt atcttgtcgt tcaataaaaa aatggagaag cataacctga cggtaacagg
    22561 tgcttcaact tttgagcgga ccaatggcga atggaatagg gcagaagcta aaacatttcc
    22621 gaatgatctt ttgcaaaacg aaaacctgca atcagccgag caaattatgc cggtagtggg
    22681 aagtcgtttt cagtcaacac tcatatcttt tctgggaaga gtcaactatt cctttgatga
    22741 taagtacctg ctttcccttt cttaccgcca ggatggatcc agcaagtttg gacgtgataa
    22801 caagtgggct ggcttccctt ctgcaggttt tgcctggaag ctgcacaaag agtcattcat
    22861 gaatcgggcg gttgcaatca gcaatctgat actccggtta agttatgggc agaccggtaa
    22921 ccagggtatt ggatcctatg cctcactgtc taagctggct gtttataatt atccgttcaa
    22981 cggttcttta caaacagggt tggcggatga tgtatatgcc ggcccggcca atgcctcatt
    23041 aaaatgggaa acaactaagg cttataatac aggggttgac cttgggttgt ttaaaggtgg
    23101 tttaaacttc catgcagatt tttacctgaa ggaaactaat aaccttctgc aatatttgac
    23161 aactccgtca tctaccggtt tccaacgtca attgagaaac tctggttctg tacagaacaa
    23221 aggagctgag ttctcactcg atggcaaact gatcagcaga aaagattttc aatggaaatc
    23281 tgccttcaat attgcattca acaggaataa gatattaagt cttggtagcg acgtaaaaga
    23341 gcagtttgcc agtaatattt ctacccggga tgctcctttc atccagactg ctggccagcc
    23401 tatcggtgcc ttgtatggat atgttgagga tggttattat gataatgaag ctgaagttcg
    23461 aagttcgcag gtttattcga gccagcccat ggcaatcatc agaagaatgg taggggagat
    23521 caagtatatg aaccttgaca atgacccaac ctctatttca aattccgaca gaactattat
    23581 cggtgatgtg aacccggatt acacatttgg tttcaccaat aatttcagtt ataaaaactt
    23641 tgatgtgagc atttttatca atggtgtaca gggtaatgat gttgtaaata tgaataaagt
    23701 atttaatggc aacattggca cttcaaaaaa cattagtgaa aagatgctgg aaggtacatg
    23761 ggtagatgga aaagataatt caaaggcaac agagcccaaa gccatccggc agttttggag
    23821 aacattgctt ttctccaggc ggtttattga ggatggtagt tttgtccggg tgaagaatat
    23881 aacaatcggt tataatgttc ctggaaaact gataaaaggc attagctcta tgagattatc
    23941 tgtaggagta aataacctct acactttcac caattattcc ggttttgacc ctgagatcaa
    24001 cagctatgga gataatcctg ctttatttgg tgtagacctt ggaggatatc caaatacccg
    24061 tagttataat ttcacactgc gctgcagttt ttaatcacag ctaatcatga aaaaaaaata
    24121 tatgcgacct ggaaaaaaat atttatggat actgtcagtg tcaatactga tgctgtcaat
    24181 gtcatcctgt aaaaagcaac tggaagaaaa accttactct tttctttcac ctgagaattt
    24241 ttacaaaaac gaaagcgatg ccaagaccgc catcaacggg gtgtacagtg ccttatattc
    24301 ctgggacctg tacctgcaac ccatgtggaa cctgacaata ctggatgatg accatgtatc
    24361 gggtgttgat tggtttctgg gaacatcagg tgccggaaat ccacagggtt attggggagt
    24421 ggatggtccc tgggtaggat gctactcaat cattgcaaga gctagtactg tgctagagaa
    24481 cgtcacaaag ataaatgaga acatagaccc ggatataaaa acgaggatat taggcgaagc
    24541 acattttcta agagcctggg cttattttca gttagtacaa ttatatggtg gagtgccaat
    24601 tcgtttacag tctttatcag ttgatcctaa tactaatgtt ccaaggtctt cagttaaaga
    24661 agtataccag gtaataataa atgagttgaa acttgctgag accatgttgt tcccggcagg
    24721 ccatgccaag gctggtgaac ctggaagagt aacgcagggt ttagcaaagg gtatgctcgc
    24781 aaaagtttac ctgacaatgg cgtctggttc ggtacctgct gctaatgtca ctgttcgggg
    24841 aggaaccgat aacggctttt acacttatgc taaatcagta gtggcaggcc ttgaaggact
    24901 ggacagtaaa acttattttg aactggcaag ggataaagct ttggaagtga tcaatagcgg
    24961 gcaatacacc ttgtttacaa gctggaaaac tatgtggagt aaagcagctc ataatacaca
    25021 ggagcatatg tgggagctgc aatccttgtc aggtacaacg tttgtcaata acattcataa
    25081 ttatttttca gccctttcta catttggaag gggagcggta tggtttacca acaatcatta
    25141 tatggattat gaagatccgg ataagagggt gctggatgga gtagcgcata attatgcaac
    25201 gaataccggt acgaggtatt attatccttc ctggcaggct gcattgtacc agacagtagg
    25261 tgggctgaca tacaataata atggaacaac agataacagg gcatatgtga tcaaatatgc
    25321 tgatgtgaca gatccaacag tggcaaatag tgatgcttat ttcccaatga tgcgttatgc
    25381 cgaaatgttt ctgatttatg ccgaagctgc caatgaagct aatgggagcc caacaacgga
    25441 agcgtatacg tatcttaata caatccggac aagagctgct gcttcagctg ccccggcagg
    25501 cctgagccag gaacaattcc gcagttttgt actggcagaa agagcaagag agtttgtatt
    25561 ggaaggcatc cgccgatatg acctgatgcg ttggggtatt tatctgcagg tgatgaataa
    25621 gatatcggca gggcaaaata atatttccaa agtaagaagc ctacgtaacc tgttattgcc
    25681 gattccgcaa agcgaactga attctaacca ggccatcacc ggtaataatc ccggctggta
    25741 aatcaacatt cataaacttc taatgccatt tatatgaaaa ggaatttatt gatacataat
    25801 aaaattctac cggttatgat gagtatgggg ctaatattat tttttggctc ctgtaaaaaa
    25861 gaaaatacgg aagacaggaa tcctccggag attaatgccg taacggacct gatgaaccgt
    25921 gatattgcac tgacatctgt taactatggt gactggatca ttataaaagg aaaacacctt
    25981 tccactacct acaaagtaga attcaatact atattggctg aggattcatt gatctatgca
    26041 gatgatacga ctgttaccgt aaagatacct gcggtacttc ctgatcccgc caataacccg
    26101 attaccgtaa ctacaaaata tggttcggca acgtataact tcagaatttt acaaccaccc
    26161 ccaaccattc tttcttttga tccaatggct ggaccggaag gccagctggt tactattacc
    26221 ggttacaatt ttggcggtgt caatagtgtg aagtttggga caaatgaggc aacaattgtt
    26281 tcaaatacaa aggaagagat caaagtaaat gtgccggctg gtgtaacggc cgaatatatt
    26341 tttgtgacaa caccaagcgg tacggttcgt tctgataaat tatacggatt ccgttacgaa
    26401 gtgtttacag atgcattaac caccggatgg agttttagtc tttcttctgc taatgtaggt
    26461 tataatgcag caattgcaga tacagtaaaa aggggatcga gatctgtcag gatagatttc
    26521 aggggatctt tttctttttt acggtttgtt aaaacggcag ctatgagtac aaccggattt
    26581 cagggggtta agttttcgat gtatgcaccg gcgaatttca tggataaaaa agtaagagta
    26641 tatcttaata attcttctac gccaggttct tacaccatta atgtgtcaaa agtgaatcaa
    26701 tgggtagagt atgagatacc actaatcaat ttcgggaacc ctgcgacact aacacatatc
    26761 atatttaatg agtttagcgg aactgctggt ttaccccggc agttttatgt ggatgacctc
    26821 ggcttgtatt aacaggcagg ccactccttt atgtaacccc ggcaggataa aaattatgag
    26881 taaaaacaga tttagttaat gatcaggatg gtaagttttt ttggggtgtt attttgctgt
    26941 atactgttga atacagcaag agggcaagtt tgttcagata aaattgcaga ctcagctgtt
    27001 aaggctttgt ataaaaattt atttacactg cagcatcagt ttacaatttt tggccaccag
    27061 gatgcattgg cgtatggtgt tggctggaaa ggtatcagca agcagagtga tgtaaagtca
    27121 gtggcgggtg attacccggg tatttacggc tgggatatcg gtcatcttga gctgcaaagc
    27181 ctgaataaca ttgataatgt ttctttccgt aaaatgaagc agtatatccg tgaaggatac
    27241 cagcggggtg gtgtcatcac catcagctgg cacctgcata atcctttaac cggaggttca
    27301 tcgtgggata ctacccatgg taccattgct tctattttgc cgggtggcaa taaacatgag
    27361 ttatttaaaa catggctgga ccgggtagct gcgtttatga atggattgaa agataataat
    27421 ggtcaattta tacccgtgtt gttcagacca tttcatgaat taacgggtaa ctggttttgg
    27481 tggtgtaaaa atgttagtac ggccgttgaa ttcaggtcac tctggcgttt tagtgtgaac
    27541 tacctgcgca aggataaagg gatacaccat ctgctatatg tatacaacac cgccaatttt
    27601 gacaacgatg cggatttcct ggagcgatat ccgggagatg acgtagttga cctggtgagc
    27661 ttcgatatgt atcagcatgg gaaaaaggaa gataagcagg aatttattga tgcaatgagg
    27721 aaacagatac gcatactttg taatgtggct gctgagaaaa ataaaattgc cgccctcgct
    27781 gaaacgggca tggaagctgt tcctgacaac gagtggtgga caggtgtttt gtggccggta
    27841 atcaaagatt tcccgctttc ttatgtactg gtatggagaa atcatggcta tatgcaaccg
    27901 aaaaaaaaaa tgcattaata tgcaccctat cccgggcagt taacaaccga taacttcaaa
    27961 aaattcacaa ctaatcccag gatattattg gagaagggtg ttaaaatgaa aaaggtgtat
    28021 caataatcag gctactaatg aaactgcaac tgctggatat tcttattgtg attacttacc
    28081 tggctacaat ggtcttcatt ggtgtttata tgcgaaaaaa ggccagggaa aataaagaaa
    28141 gctacctgat gggtggtaaa aaactgccct ggtatatgct tggtcttagc gatgccagtg
    28201 atatgtttga tatcagtggt acaatgtgga tggtggcctt gtgttttgta tatggaatga
    28261 aaagtatgtg gataccctgg ctatggccgg tattcaacca ggttttcatg atgatgtttt
    28321 tatctaagtg gttgagacgc tctaatgcga atacaggtgc ggaatggctg gcaaccagat
    28381 ttgggctttc tggaaaaggc gtaaagggat ctcataacgt agtggtgata tttgctttaa
    28441 tcgggtgctt tgggtttttg gcgtatggat ttatcggcct gggaaaattt atcgaaatat
    28501 ttgttccatg ggaaatggtc agtggatata ttccttttaa cattgctcct caatatgcag
    28561 cgcacttcta tggaatagtc tttacgctgt ttgccatgtt ctattcgatt attggcggtt
    28621 tgcatagcat tgtcctggga gattttatca aatacataat catgacagtg gcctgtgtgt
    28681 ctattgcagt gatagcaatg atccatttac atcaaaacgg gaaccaactc cgggttcctg
    28741 atggttggct gaatcctttc tttggttggg atcttgatct tgactggagt ggaattgtac
    28801 cggatgtcaa taaaaaaatt gaagacgacg gttacggttt gtttgggata ttttttatga
    28861 tgatgttgtt caaaggcatc tttgcgagcc ttgcaggtcc ggcacctaat tatgatatgc
    28921 aaaaggtatt atcaacccgc tcgccgaagg aagccagtaa gatgacaggg tttgtttcta
    28981 ttatcttatt accggttcgt tattcaatgg tcataggact tactatatta gcattattgt
    29041 attacaacca gttgaacctt tccgcaccgg gcggaggaac agattttgaa aaaatattgc
    29101 cggcagcagt aaataatttt ctgccggtag gtgtattggg actggtactt acgggactgc
    29161 tcggtgcttt tatgggtact ttctccggca cactcaatgc tgcacaggct tatattgtca
    29221 atgatgtgta tttgaaatat ataaacccgg atgccagtaa taagaagatc atccgcacga
    29281 actatatcgt tggtgtattg gttgttgcta taggtgtggt gctgggattt tttgccaaag
    29341 atgtgaacag catattgcag tggatcgttg gtggtcttta tgggggctac atagccgcca
    29401 accttttcaa atggtattgg tggcgtttta atgccaatgg cttcttatgg ggtatggcaa
    29461 gtggtatcct gggagcattg gtatttttaa aaatctatga tggacttttt ctttatatct
    29521 ggcctgtatt atttattatt tctattgcag gatgtctgat tggaacctac acggctccgc
    29581 caactgaaat cgatacactg aaatcctttt ataagtctgt caggccatgg ggtttttgga
    29641 agcctgttca ccggttagtg gtagaggagg atccttcttt tcagccaaac aggaggttca
    29701 agctggatat gtttaatgtg gtgttgggtg tcatcgcaca atgttgtttg accatattac
    29761 ccatgtatat cgttttgtgg atgaagctgc ctttactgat aaccatttcc atattactgg
    29821 tcattgtatt gatccttaaa cgtacctggt gggataaact tgaagactaa tgttttaaaa
    29881 cttttttgta tgaaaaatga atttaattat cggttatctc aactggaagc aatgcaggaa
    29941 ttgcttctga caaaatccaa tgaaatggat ggtgccggta acgggctgta taaccgatac
    30001 aaaaatcctg tattgactgc ggatcatgta cctcttaact ggcgatatga tttcaatgag
    30061 aaggataatc cctacctcat tcagcggttt ggtatcaatg cagtgctgaa tgccggtgcc
    30121 attaagtggc ggggtaaata tttattaatg gcccgtgtgg aaggatatga ccgaaaatct
    30181 ttttttgcta ttgccgaaag cttgaatggt attgataatt tcagattttg ggattacccc
    30241 gtcaatatgc cggaaactgc tcagccggat gttaatgttt atgatataag actggttgaa
    30301 catgaggatg gctgggtata tggcttgttt tgtacagaac gacgggcagt gggggtgcca
    30361 gaatctgatc aatcttctgc cgtagcacaa tgtggtattg cccgcacaaa agatatgcta
    30421 aactgggaaa ggctggcaga cctggtaaca ccttcaccac aacaaagaaa tgtggtatta
    30481 caccccgagt ttgtaaatgg gaaatatgct ttttatacaa ggccacagga tgggttcatt
    30541 gatgccggca gaggcggagg tataggatat ggccttgctg aaaatattga aaacgccgtg
    30601 attattgaag aaaaactact tgatagaaag ttgtatcata ctgtatatga actgaaaaac
    30661 ggattgggac cggcacccat caaaacacag tatggatggg tgcacctggc acatggcgtt
    30721 cgaaacacgg cagccgggct tcgctatgta ttgtatatgt ttatgaccag tctaaatgat
    30781 attacccggg tgattcataa acctgcaggt tattttatgg caccagaagg tgaagaaagg
    30841 attggtgatg tttcaaatgt tttgtttagc aatggctgga ttgcggatga ggatggtaat
    30901 gtttatattt attatgcttc ttctgataca agggtacatg tcgctacaag tacggtcgat
    30961 cgattggtgg attatgtaat gattaacccg cctgacggtt tccgttcttc tgcatctgta
    31021 gctacaataa cgacaattat cgataagaac aggcagctga atgaaacatt attacagcct
    31081 ctgttggcag gggcttcaaa aaatggaaag gaaacgaagt gatagtaagg ttgacagata
    31141 gtagtgtaaa ggaacccttg cagcgatacc gttcacaggc agaagcggaa cttgcttcgg
    31201 tactagattg gtggatgcag tatatacctg atgacgaaga tggttttcat ggagagattg
    31261 acagatacaa taaattgaaa gcagatgccc ctcgtgggtt ggtattatac agtcgcatat
    31321 tatggacttt ctccgctgcc tatatacata cccgtaaccg ggaatatctt tttatggccg
    31381 aaagggctta ccgttatctt atcaaacatt tccaggatac ggtcaatggc ggcatgtatt
    31441 ggtcggttga ccggcatggc cagaagctta ttgacagaaa gcaggtttat ggtattgcct
    31501 tttgtattta cggaatgagt gaatattatc aggcaacttt taatgaacag gcaagagata
    31561 aagcaataga gttgtttcta ttgctcgaac aacatagcag ggataaaaaa gaaggaggtt
    31621 atttcgaagc ttttgccgct aattggcaac ttgtgcctga tgtccggtta agtgagaaag
    31681 atgcgaatga aaaaaaaaca atgaatacgc accttcatgt acttgaagca tacaccaatc
    31741 tttacagaat atggaaaaat gaggttttgg ctgaagcgct ccattcatta ctagttgttt
    31801 tcaaaaagcg gatcattgat acggctactc atcatcaggg gctttttttt gcagaaaact
    31861 gggaactccg ctcttctgtt atttcttatg gacatgatat tgaagcatcc tggctattgt
    31921 atgaagcatc agagatgttg ggcaaccctg aagtattgca tatttacaaa gatatagttg
    31981 tgaaaatggc tattgcggtt ttaccggcga tagataatga tggaggtatg tggtatgaat
    32041 cagaaaccag aacggggcat tgggtaaaag aaaaacattg gtggccgcag gcggaagcaa
    32101 tggtaggatt tatgaatgca taccaactaa ccggcgataa aatctacctg aagtactcac
    32161 agcgaagttg ggattttgcg aggcaatata tttgcgacaa agaaaacggg gaatggcact
    32221 ggggggtgga tgaatatgga aatatgatgg aaaaggagaa agccggtttc tggaaatgtc
    32281 cctatcacaa tggaagggct tgtatggaaa taagtaaacg tatatctgca atattacaat
    32341 aaggtctgca tcagtttatt taagggtaat cttttactgt ttttctgctc tgcagataga
    32401 gtgttgataa ttaatgcaca ttttatcttt tcctttttaa taacaactat tacagccccg
    32461 gtcgtagaca ttcattctta ttattaacag tttttcttca aatttgaaga gaattggagc
    32521 caatgatgca gtaaggacaa tcaaaaaggt tttagatgaa gcggtaatat ttccaggttt
    32581 accgggcttt ttttttgagc agggaaaaat gatactttac ggcaagttca ggggatctaa
    32641 tttttataaa gtggagtatt tactatgctg tgaataatca aggctatgta aaatggacat
    32701 ttatctaaat ctaaaataat ggtggttatg cagatcaaaa atgcctttat ccctgtggtt
    32761 atgggactgg ttatgtttta ttcatgcaat gaacaacaag atactggcgg taaagtaatt
    32821 ctttataaaa atgccaaaat ctggactggt gacagcagta atttatcagc ctcttgcata
    32881 gccatccggg gtgaatccat tctttatgtt ggatccaatt cagacagtat caaagcggat
    32941 tcagtagtag accttcaggg aaggatgatt gtgccgggtt tcattgataa tcatacacac
    33001 tttctcagtg gcggttgcca gttaaatgca gtagacctca ggaaagccaa aacaaagaat
    33061 cagttcatcg caatacttaa tgactatatc acggctaatc ctggcgtcac ctggatccag
    33121 ggcggcgatt gggatcatga agtctggggc ggtgaattac cagtcaaaga atggatagat
    33181 agtatcactg gcaaccgtcc ggtatttttg tcacgatatg acggtcacat ggccctggcc
    33241 aattcagctg ctttgaaatt ggcaggtatc gataaaaaca caaaaagccc tgatggaggt
    33301 gaaataataa aagatacaca aagcggtgag cctggtggtg ttttgaaaga tgatgccatg
    33361 ggactggtgt acaataaaat acctgatccg acttcgcagc aacttgataa attactgcaa
    33421 cgggcaactg gacatgctca taaaaatggt gtaacacaag tgcatgatat gggcagctat
    33481 ggtggttgga ttgatctcga gacttaccgc agggcctata cgaaccaaac acttggtatt
    33541 cgaatttatt cttttgtttc gctgagtacc tggcaaaagc tggctgacta tgtaaaacag
    33601 aatggaaaag gagatgacct gttacgctgg ggaggtttaa aaggttttgt agatggatca
    33661 ttgggttcaa caactgcctg gttttataag ccctatctcg atgcccccaa aagcaccggc
    33721 cttgtgctga acgatacaaa cgtattgcgg aaatggatac ttaatgcttc tgccgaatac
    33781 ctgcaagtgg cagtacatgc tattggcgac agggccaatg actggctttt aactgttttt
    33841 gaggaggcca aaaaaatcaa tggaaaacaa agcagccgtt tcagaataga acatgcacag
    33901 cacctgagtg aatcagcaat accaaaattt gctttattaa atgtgattcc ttctatgcag
    33961 ccataccatg ccattgatga tggccggtgg gcagctaaaa gattagaaga tgaccgattg
    34021 aaaagaacct atgctttcaa atctcttttg gatgcgaatg ctaaacttac atttggttct
    34081 gactggactg tggctccgct caccccgtta gaaggcattt atgcagctgt tacccgcaag
    34141 actttagatg ataaaaaccc ggatggatgg taccctgaag aaaaaattac agtagaacag
    34201 gcattgcgtt gttatacaat caataatgct tacgccgggt tccaggagaa taaattgggt
    34261 atgctgaagg caggtatgct tgctgatttt gttgttttaa gcgatgattt gttcagtatt
    34321 cctgcagaaa aaataagaga tgcaaaagtt ttaaaaacca tctctaatgg aaaactggtt
    34381 tataattcaa atgaatccaa aaagtaattt tatttcccgg ggttcttaca aggcttttaa
    34441 taccgggtag aaattgccat tataaatatc aggggcaatg tacaggaaag tgagctaccc
    34501 tattttaagg atatacttga ttttttctct taaaagttaa ttcactaccg gtcttttcat
    34561 cgttccgagt cctttttctt cattgagtat acgataattg atgagtcggt acttcggatc
    34621 acggaggagc tcttgcagtt gcggggaaac cattgttttt agttctgcat aacggtgctt
    34681 gctcatctct ttcggccccg aaggattgag atcttccatc gcactcattt ccggatcatc
    34741 aagtccgata tgaacaacaa ataattttgt gccgccgggc tgcaggtttt tcacttttat
    34801 caacatcgta tccagtttat tgctgacggg agcggagtat ccgccttcaa catctacttc
    34861 atcataatag cgggaaatag caacattata ttcagcagca agtttttcaa ctatggctct
    34921 tgtttccggc gtttgcatgg cggctcccat atgatagtca agataatcga tcttcaaacc
    34981 tgcgagtagt gctttatcaa tctgtgcacg gagttctgtt tcaatctcac tgagtttcgg
    35041 gttattgccg aagagtttac tgcgggaggg aaagaaatag ccgaatgagt caaccagcgt
    35101 tggaacagcc attgggcccg acactggccc ccagcggtat tgcttccatt ctgaattgag
    35161 cgtaaggtga atgccaacgg agacgtttga ataatttttc agtagcgcta ctccttcgga
    35221 aaaccaggga cagggaacca ttacagacat cgaaacagga ataccggttt ccagtacttc
    35281 tttggcggcc atattcacag catgacacat accgatatca tctaaacgga aaaggatggg
    35341 aatcttgtta tcaggattac ttttctgtgc aaaggacagc gaagaaaaat tgaagaagat
    35401 caatactaat gttatacgtt tcattgtgat gatatgtttt ttaatagtaa aacgaaaacc
    35461 gaaaataaac aagatctgca aagctcaaac taccttactt tgaatttatt aatctgtgac
    35521 ggcttctaac ctgtacttcc aacaggttct tgtattcgtt attctcacat taattaagga
    35581 tagtaccgga ttatgaatct atatgaagtt aataaaattc ttcacctgca gagtcgtttt
    35641 ccatcttcac tttctaaacc taaatcttta atttgtaaga atttgtgcta cttgaaaaaa
    35701 ccggaactga aatttgtgtt gagcagaaga gtttatcaga aaaccggtgt tttcatttaa
    35761 tgacgactat caccagccac actgaccgtc ttcattcaaa aagtccaatg ttttcagcac
    35821 atttggtaag aattaaaact tgtggtacaa accagacctt ggaggcagtt tctggatgag
    35881 gccggggata tttcccgttt tacaggaaga tttttttccc aatggttccg gccacgatac
    35941 gaagtggcag aatttttcag gcaatgtttt attatcgggt acaaatcatt accgctggtt
    36001 ggattgactg gttttatcat ggggttggta ttgacgatgc aattgcgtcc ttctttagta
    36061 agttatggag tagaatcaca actgccggct atggtaggca ttgctattgt cagggagata
    36121 ggtcctgtta ttgctgcttt gatatttgcc ggtaagatcg gaagtagtat cggtgctgaa
    36181 ttaggttcta tgaaagtaac agagcagatc gatgctatgg aagtctcggg cactaatcct
    36241 ttcaaatacc tggtagtgac aagagtgctg gcttccacac ttatgttacc tattcttgtt
    36301 atactcgcag atgcagtttc attgtatggc gcttacctgg gtgttaatat tcgcggtgta
    36361 accagtttcg atttattctg gcagcagata tttgacaatc tctcttttgg tgatgtggtg
    36421 cccgcttttg ttaaatctta cttctttggc tttgctgtgg ggattgtggg ttgttacaag
    36481 ggctataatt cccggaaagg aacagaagga gtgggacgct ctgctaactc agcagtcgtt
    36541 ctttcctcta tacttatttt tatgattgac ctgattgcag tacaaataac agaccttctc
    36601 catcttaact aatttttatg ctggcaacag cttcgatagt aacagaaaaa aaaataataa
    36661 cagaaaagga gaaagttatt gctataagtc acctttataa atcattcggt gataataaag
    36721 tgctggttga ttttaacctt tcactttatg ccggtgaaaa tgtagtagtg ttagggaaat
    36781 cgggttcagg aaaatcagtt ttgataaaat gtattattgg cctgctggaa ccagataatg
    36841 gaaccattga agtatttgga aagaatattc ctgacctggg acatgatgca ctggatgaga
    36901 tgagggtaaa agtgggtttc ctttttcaaa gcaatgcttt atatgattcg atgaccgtca
    36961 gggaaaatct tgaatttcca ttacggcggc atactattaa aatggcacaa aaagaaacag
    37021 acagacttgt aaaagatgca ctggatgatg ttggactggc gcatacaata gatatgatgc
    37081 ctgctgaatt atcaggcggt atgcgtaaaa gaattgcact ggcacggaca cttattttga
    37141 aaccagctat catactttat gatgagccga caacgggatt ggatccgata acaggtaaag
    37201 agatcattga tctgatgatt gagattcaga aaaagtataa ctcggcttcc ctgatcattt
    37261 cgcatgatat gaattgtgta cggatggcat cagacagggt gattatgctg attgatggta
    37321 aatgttatgc cgggggaacc tatgatgaac ttaaaaaatt aactgatcct aaaatcaaag
    37381 aattctttga ataaatttta tgagcaagaa agtgatttca aatgttaagc tgggtttttt
    37441 tgtgatagca ggtttgctgt tcctgatcct gctgttgtat atgattggcc gtaatgaaag
    37501 catgtttgga gactacttca ggctaaaggc caggtttcat aatgtacagg ggttaaattc
    37561 agggaataat atccggttct ccggtataca ggtaggcacc gttaaaaaag taaaaatatt
    37621 gaatgatacg ctgattgaag tggacctgct gattgaatct aaaatgaaaa attatataca
    37681 taaaaatgca attgtgtcta taacgactga cggtttaatg ggaaacaggg tactggatat
    37741 tacaccttcc aaagaaccag ctgccctggt agaagaagac gatatcctgg tcactaaaaa
    37801 aacagtggat atgggtgata tgctgcagac actggctaaa acaaataatg atgttgcatt
    37861 tattgcagcc gaattaaaga gtaccatgca acgggtcaac ggtagtactg ctatttggac
    37921 attattaaat gataatacac tgccagaaaa cctgcgggca tctttgtcta atgcaagggc
    37981 agctacggca aaagccaata gtatgataca tgatctcaac cagttggttg tagatatcaa
    38041 agagggaaag ggttctttgg gagcattact cactgacact gtttttgcca gtaatctgaa
    38101 tgatgcgatt ataaaattca aaactgttgg agaagatgct gacaagctgg ctctggaact
    38161 gaattcactg accaaaaata tccggaacga aatagataat ggtaaagggc cattaaacac
    38221 cttgttaaaa gattcagcta tggttcgtaa tctgaatcag agcatagaga atattcaaaa
    38281 agggactgcc ggttttaacg aagttatgga agcgatgaaa cacaatttct tattaagggg
    38341 atatttcaaa aagctggaga agcagaaagc aaaaggtatg tagacgccgg aacctgtatt
    38401 tattacattg ctgtattcta aatacaatgt attattacac cgttgattaa tttctttatt
    38461 ttcggtatag ctgcccttca tatcggtcag taataaatga tatgagcatt attcgtttag
    38521 ccaggtcatc tgatgcggaa ggaattctgg caatatatgc accgtatatt gaaaggactt
    38581 cattcacttt tgaaacagaa gtgccttctc ctgaaatatt tgaaaacagg atcaattctt
    38641 atcttgaaaa ctggccctgg ctggtatgtg aaataaacgg atcgattgct ggttatgcct
    38701 atgcatcccg ctatcgggag agagcagcat atcaatggtc agtggagtgc tctgtttata
    38761 tacatgatga ttttcatcga cggggagtgg gccgggcctt gtatgctgca ctatttgaaa
    38821 tattgaaaaa acagggattc cggaatgtgt atgctgttat caatctacca aatatgaaca
    38881 gtgtggcctt tcatgaaagt tgcgggttca attattttgc cacctatgag aaagtgggct
    38941 ataaattggg ccaatggaaa aatgtgggct ggtggagatt gattttaaat gaatttaata
    39001 atgaaccggc tgcacctgtc tgcttttctc aattggagaa agatttcctg ccggatgttt
    39061 ttcaaaaagc aatactcctg ctccattaaa aaaaagccgc cccaataaag gggcggcttg
    39121 aatatagcgt gcaatcgttc taaaaactaa aggcgatacg gacaaacaaa cgacgtccgg
    39181 ctgttcccat ttgcacagca tcccatgcac cacctgtttc tgtattatag gcccagtctt
    39241 ttgctccttt taccacacca aaatcagggt gtgcattcag caggttgtca acacctgcat
    39301 gcaataccaa ttttttaaat atcgggtggc tggcataaat atctgtcacc agttttcctg
    39361 aatagtcaaa ctggtctggt acatcaccac tgccattgtc aagtggtact gttggagaaa
    39421 tacctaaccc gtcctggcca taacctagca atgtaacttt tccaaaatag gttaaccttg
    39481 tacccagtgt cagtttcttt ctgccgtatt caaaacttaa tgctcctttc atatttggtg
    39541 cagaagccaa aatgaatttc tgttccctgt cactcaggaa tgtttgctgt aaaaatgcag
    39601 acccactgag tttatcaggc acattgatct tatcaatttc catattctga atattacctg
    39661 caaacaaggc acggaagcgg ttgtctccta cttttttatt gtaatctatc actacatcaa
    39721 cacccatatt ggttgtattg acagcattgg caaagaattg tgcatagcta acatttagtg
    39781 cagccatctg tgaggccagt cccggatcaa gactgggatc actgccatca aattgtccgc
    39841 ttatcactac acggtctttg atctttgtca tatatccatc taaagttaca ttcagggttg
    39901 gcgttgcttt ccaggtaaaa ccaacactgg cgctgagact tttctcctgc gtcaggtcag
    39961 gtatacctgc agcttttgcc agcgaactgt aatttggtgc aatctttact tctgcaatat
    40021 ttcctgcctg tacggtagtg aaagtagagc tgaaatttat ctgttgtaat gatggtgctc
    40081 tgaagccggt gctgaaggaa cctctcaggt taacagtttt agaagcttta acccgggtgg
    40141 caaatttgta gttatgtgta aaaccaaaat cgctgtagtt ctctaaacga acagccaggt
    40201 taaccagcca ggccggaatg atatcaaatt cagcatcagc ataagcgcca aacactttac
    40261 gctgtgcatt cacttcatca ttaggttgat aaccggggaa gccctgtgca cctgttgctt
    40321 tatttccgga gaggtcataa ttagtatatg aagctgcttc acctgcatat atctggtaat
    40381 tttcataccg gaattcggca cccaaaccaa gattgaaatt tttggcaaat tcacgactga
    40441 agttcaggtt agtggtgttt tgtaaaaaag aaaaacctcc gtcatcaaaa aaggtttgag
    40501 cagaacctaa agaggcatta aatgttttat ctccatagaa atgaaaattg tttttaccaa
    40561 cggtattgct aagatcccag ttccagttgc cccaggccac acttttcact cctcctgtta
    40621 tggagaaatc tttgatcttg gtttgaatat gcgggctata ccatatctct ccgtcacttc
    40681 ctgttctcat tatttcggga acaaaaacca aatcgccgaa tgcatctgta ggaaaacgat
    40741 cgggccttgc agaccagtta cgggtgaatg cataagcatc agatgattta ctgttgtggc
    40801 caccatagga atataaagtt gcctttcctt tggtaggcaa ttccaggttg taaaaagtgc
    40861 caaaagtgtt taccccggca tcaccatgcc cccggcgata aatattcagg tacatagcat
    40921 catcgtctga gatataatta tcagtcttta atgcctggcg gaatgttttt gaagtactca
    40981 cgccttcgaa agtgaggttg ataaaaccac ctctttctcc aaaaggtaat ccgaaattca
    41041 cattggcagc aagtgacttt ccgtcaatct tacctgcatg ttcataatcc tggtgaagac
    41101 cggattcaaa agcagtattg aattttggat cataataacc agccacgcct acagaacccg
    41161 ttatctggtt cacagatttt ttggttatta agttgataac accagcaatg gcatccgaac
    41221 catattgggc ggaagcacca tctcttagta tttccactct ctcaattgca cttgcaggca
    41281 atgcattcag gtcagtgcct gaattaccgc gacctcttgt gccaaacatg gctacaaaag
    41341 ctgtctggtg acgtcgttta ccatttacta aaactaatgt ttggtcagga ccaaggccac
    41401 gtaatgaagc gagatcaaca tggtctgcac catcactgcc actttgtttg ttataattga
    41461 aggatggggc tgaataattt aatacggatg tcagatccat cctggcggta gaggatgtgg
    41521 cctgtgatac attgattaca tccacaggag cagtggtttc tgtttttgcc cggccggctc
    41581 ttctggaacc caccagtacc acctggctca gttcttcaat agattgggca agggtgatgt
    41641 ttaccgggct ggtaacattg gctttgatgg tttgttttac aaaaccaatg gatgaaattt
    41701 cgatttcatc atccgaatta acggtcaggc tgaatgttcc atcggcgcct gtcagcgcca
    41761 tcttaccagt tttttttgat ttaaccgaaa cgcctgccac tggtaacccg ttagcatcgg
    41821 ttactttacc ggttacgggt tgctgtgcta tgacagtcac tccgatacac ataaaggtga
    41881 gtaatagaaa gagagccttt ctcatataag ttagttttgg cctgtctgcc gacaggtaga
    41941 ttaaaagatg acttaaatat aatggaaatt atattgaaat gaaatggctt tctattaagc
    42001 tatgtgaata gattaacctg caattgtgta aataaaagat taaaaaggta ataactactg
    42061 aatatatacc tgcataaata tgtaaactgg cgggttaagc agttcatatg ttgtaataaa
    42121 aaaaagaggc tgtctcaaaa gcccctatca gtgtcatatt gagcatagcc gaaatacgac
    42181 actgataggt aaacaaattt gtactcattt cgataaactc aatgtgacaa atttgtttgc
    42241 ttttgagaca gcttctttca aaaattataa taggaccctg gcattattgt tcagctacag
    42301 cagttatttt ttctgtaact acttttccag ggtatacttc caatgctttt tccaggcaat
    42361 ccatagctgc attgattgca tcaaggttta acacatatgc taaccggact tcatgcttac
    42421 ccaatccggc agtaccataa aagccggtag caggagccag catcacggtc tggttgttat
    42481 acgagaattc ttccagcatc cactgacaaa atttatcact gtcatcaata ggaagttggg
    42541 ctatggcata aaaagcaccg ccaggattag ggcaaaaaac tccctgcatt gcattcaggc
    42601 gttgaactaa taaatttctc cgttgcaggt attctgcttt cggggcatca aaataactgt
    42661 caggaagatc aacagcggct tcacccatta tttgtgccaa gcccggcgga ctaagccttg
    42721 cctgtgcaaa cttcatcgcc acatcataga cggctttatt tttcgtaaca aatgcaccca
    42781 gcctggcacc gcaagcactg tatcttttag aaatagtatc catcagcacc acatgctggt
    42841 ccagaccctg gaggtgcata gcactaatat attcaccatc gtagcaaaac tcacggtatg
    42901 cttcatcgct gaagaggtac aggttatttt tgatacatat ttcctttaga gcttccatct
    42961 catccctgct gtagagatag ccagttggat tattcggact gcagatgatg atagcttttg
    43021 ttttagcagt tatcactttt tcaaaatcat gaatgggagg cagggcaaaa cctgtttcaa
    43081 tgcgggacgt aattggcacc acatttacac cggcagcaca ggcaaaacca ttgtagttgg
    43141 cataaaaagg ttcaggaatg atcacttcat cgcctggatt gaggcaggta aagaaaccaa
    43201 acatgatagc ctcgctgccg ccagtggtta tcagtatctg gtcatgactt acatcaatgc
    43261 ccactttctt ataatattgc accagtttac ggcggtagct ttcattgcca gcgctgtgac
    43321 tgtactccag tgtcttgatt tcggcatgtc gaacagcatc cagtatagca ggtggtgttt
    43381 caatatcagg ctgaccaata ttcaggtgat atactttaat tcctttcttt ttggctgcct
    43441 cagcataagg caccagtttc cgtatcggtg aggggggcat ctggttaccc cgttgggaga
    43501 ttgttagcat agcacaaagg taacaactgg ctggcgaata acagagatag cagtaggaaa
    43561 cctggtactc ttcatttttg aagtattaaa aattaaatca taaagttgaa gagcatatac
    43621 cgctggctga atggttgtaa aataaaagag tcccttcaca attctgaagg aactcttttg
    43681 tatctccaat cgattaaatt tattaccctt ttttcggtgc cggagttttt gctccttttt
    43741 cttttacata aatttcgtat gcctctgcac tcagtacttc accggtaatt tgtaatgtct
    43801 ttgtttgttc tattccggca aatttgatat aaacatcttt tgtgaatggt gcaacagcag
    43861 ctgcattata ttccactttc agttttgcaa cgcctccggg cataatggga tcgactattt
    43921 tttcaggggt agtacatccg caactggccc aggaattttc aaccacgatg ggtttatcgc
    43981 ttatattttt tatttcaaag taataagtaa taggtacgcc ttgttttatt ttaccaaagt
    44041 catgtttttc agtattcatt ttcacggtct cgtctacttt agtttgtgca aaggctccga
    44101 gtgagaaaac aaatgctgtt gcaaggaaga agattttttt catggtttat tttttaaaag
    44161 gtttgttttt ttaacagttc aacagaagaa ttggaaggtg caggtccctc cggggctttc
    44221 cacacagttc cttttatcct gatttgtttc gtttgtgttg tgttgtaggt tatagtaata
    44281 aacttgtcaa aaggcccttc agcagcagaa ttgtaaccta cttttatttt cgctgtagcg
    44341 ccgggtgcta ttggatcctg gctccattcg ggagtagtac aaccgcaact tgcctgaaca
    44401 ttatccagtt tcaatgctat tttcccggta ttcacaattt caaatgtgtg ataaactggc
    44461 ttaccctggg gtatttttcc aaattcatat tctgtttcct tcagtttaag cgcatcttca
    44521 gaaacagttg caggcttggt aactgtacca tgattatgtc cgtcattttc agaatggaca
    44581 ggctgctgtg cacaaagacc tgtggcaatg gtaagtgaaa gtaacgatag ggtgattttc
    44641 ttcatactga agcgtttagc gacgtcaaat ttagtggaaa gatggcatat tacatagggg
    44701 ggttgcaggg gttgtgaggt attttttaac agggggtttc tgcggggtca atctccctat
    44761 ttttgctgta tggaaaatac taaccccggc ataataacag acactgataa actgtctttt
    44821 gacaggttca gggaggaagt attaaatgat ttccgtacag tttgtataag cagggaaacc
    44881 agtctattag gcagaaaaga agtattgggc gggaaagcca aatttggcat ttttggagac
    44941 ggtaaagaag tagcacaggt agcgatggcc aaattctttc aaccaggcga ctttcgtagt
    45001 ggctattacc gtgaccagac ttttatgatg gctgccggat tagcaactgt agaacaggtg
    45061 tttgcacagt tatacgcaga cccggacctt gacagggagc cttttagtgc aggccggcaa
    45121 atgaattgtc attttgcaac aaggatggtg aatgaggaag gcgagtggtt gaatcttgcg
    45181 gatttaaaaa atatttccag cgatatagcc cccacagccg gacagatgcc aagggcattg
    45241 ggactagctt ttgcatccag ggcattcagg aatattgagc agttgaagtc ttttaaaaat
    45301 ttgtcgacca atggtaatga agtttgtttt gctacgatag gtgatgcgtc taccagcgaa
    45361 ggtcattttt gggaaacgat caatgcaaaa ggcgtgctgc aggtaccgtt ggcagtattt
    45421 gtatgggatg acggctatgg gatctctgtt cctaaaaaat ttcagaccac caaagcatct
    45481 gtctctgatg cattgagtgg aatgcagaaa agagaaggaa caaatggatt taatatttat
    45541 aaagtaaagg gatgggatta tgccggtatg tgtgaagtat ttgaagaagg tatccggtta
    45601 gcgagagaaa cccacacacc tgtgcttttt catgttgaag aaattacgca gccgcaggga
    45661 cattctacat ctggcagtca tgaacgttat aaaagccagg aaagactcga gtgggaaagg
    45721 gaatgggatg gaataaaaaa attaaaggaa tggatcatcg ccaatgcatt ggcagaggaa
    45781 catgaacttg acgaaatagc tg
//
LOCUS       DAORTI010000015        40886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_580_length_40886_cov_9.614680, whole genome shotgun sequence.
ACCESSION   DAORTI010000015 DAORTI010000000
VERSION     DAORTI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 40886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40886
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_40886_cov_9.614680"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLO70_03765"
     CDS             complement(<1..500)
                     /locus_tag="PLO70_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQZ73602.1"
                     /translation="MAQWNTTVHTYSGKKVNRVGAPGVAKHNPKIDMTPMVDLGFLLI
                     SFFVITTELSKPTAMDLFMPKESKVPMELGELNALTVLLDKDNRAWCYHGRWEDAVIQ
                     HTIIKTGFSGNNGLRKNIIAKQQWLDNYNKKEGRNGLMLVIKPGSGASYKNVVDVLDE
                     VSINGV"
     gene            625..1452
                     /locus_tag="PLO70_03770"
     CDS             625..1452
                     /locus_tag="PLO70_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ73603.1"
                     /translation="MKTIIIPTDFSPIATNALHFGIDMAKAINASILLLHVYQVPVSF
                     TDTPIVLVSIEDLRKGAEEQIEKLKKEVEHLTSGSVKVYTETRMGNVTDELETLCSKI
                     NPFAVVMGTKGSSGVERILFGSNTLNAIRHLNWPVICVPPGKAFGHGIKKIGFACDFR
                     DVVKSTPTHFIKEFVKEFNAELHVLNVDHDNKHFKPETPEQSILLHTLLEEANPSYHF
                     IEHADIEDGINEFAEKNNLDLIITIPKKHKLLQGIFKPSSTKQLVYQAHVPVMCVHE"
     gene            complement(1530..2972)
                     /gene="asnS"
                     /locus_tag="PLO70_03775"
     CDS             complement(1530..2972)
                     /gene="asnS"
                     /locus_tag="PLO70_03775"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQZ73604.1"
                     /translation="MFNKRIKIKELLAKEPAGQEVIVMGWVRTFRNNQFIALNDGSTN
                     NNLQVVVELGKFNDDILKRITTSASLKVTGTIVPSLGKGQKMDLKADTIEILGDSDAE
                     KYPLQPKKHSLEFLREKAHLRFRTNTFGSVFRVRHSLAFAVHKFFNKKGFVYMHTPII
                     TASDAEGAGEMFRVTTLPIDGTAPKNEDGSINFKEDFFGRSTNLTVSGQLEGELGATA
                     FGDIYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDLEDNMNLAEEFIKYIIKYAIDN
                     NREDLEFLAQRLADEEKQLPQDKRSEMGLIGKLEFVLNNNFERITYTEAIDILKESNH
                     NKKKKFQYEITGWGMDLQSEHERYLVEKHFKKPVILSNYPKEIKAFYMRQNADGKTVA
                     AMDILAPGIGEIVGGSQREERQDKLEQRMKEMHIPVEDLWWYLDTRRFGTVPHAGFGL
                     GFERMVQFVTGMTNIRDVIAFPRTPGSAEF"
     gene            3278..4927
                     /gene="rho"
                     /locus_tag="PLO70_03780"
     CDS             3278..4927
                     /gene="rho"
                     /locus_tag="PLO70_03780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQZ73605.1"
                     /translation="MYDILQLNDMLVPELLDIADQLKIPNAKKLSKQDLVYKILDSQA
                     IAGSKGAKPADDGKRKRIIKTSTSTGSEEAVVESGGEEKTIIKKPIMAVKKDDKTPIK
                     RARKKPEEKEITADADTAVVETQPKETEAELQTKTDQLAQAILNEGNQQSPENPSGAG
                     QNKFYPTRREQQQQFNVEFDGVILGEGVLEMMPDGYGFLRSSDYNYLSSPDDVYVSPS
                     QIKLFGLKTGDTVHGAVRPPKEGEKYFALLKVDTINGKSPEEVRDRVPFDYLTPLFPY
                     EKLNLFTTPSDYSTRIMDLFTPIGKGQRGLIVAQPKTGKTMLLKAVANAIAANHPECY
                     LMVVLVDERPEEVTDMERSVRAEVIASTFDEPAEKHVKVSTIALQKAKRLVECGHDVV
                     ILLDSITRLARAHNTVAPSSGKVLSGGVEANAMQKPKQFFGAARKIEHGGSLTIIATA
                     LVDTGSKMDEVIFEEFKGTGNMELQLDRRLANKRIYPAIDLVASSTRRDDLLLDRDVL
                     QRMNLLRVYLTDMNAEEAMRELQNRMKGTKNNEEFLASMNR"
     gene            5006..6124
                     /gene="mnmH"
                     /locus_tag="PLO70_03785"
     CDS             5006..6124
                     /gene="mnmH"
                     /locus_tag="PLO70_03785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HQZ73606.1"
                     /translation="MGVEKIHIEQFLELAKHYPVFDVRSPGEYMHAHIPGACSLPLFT
                     DEERKVVGTTYKQQSREQAIKIGLDYFGPKMRKMIEEVESICSSRNSLLTTQDSRIIL
                     VHCWRGGMRSGAISWLLDLYGFKVYTLIGGYKKFRNYVLDTFKLPFHLNILGGYTGSG
                     KTELLKALKERGERVIDLEDIAKHKGSAFGSIGMPQQPGQEMFENILAQSLHEVRGKL
                     SETGKTPSDFMAHPSGIWLEDESQRIGHVNIPGDFWQNMRQSPLYFLDIPFEERLKHI
                     VQEYGQLDPEKVADAIGRISQKLGNLNAKTAILLLKEGKITESFNILLNYYDKFYFKS
                     LHNREGVNSLLQVIECKSITPENADKLIRQANYHAERS"
     gene            6075..7790
                     /locus_tag="PLO70_03790"
     CDS             6075..7790
                     /locus_tag="PLO70_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73607.1"
                     /translation="MPINLYARLITTPKGLSLSILFLLFPIFFHAQSLTGLWVGALSN
                     DSTTTRKDQSFEIALTEYKGKVYGYSRSEFIVNDTLYYIVKRVKGIIEGDACEVSDEE
                     IIAYNFRGKLDKGIKVTSTFRRNKNDSTWYLDGTWKTNATRKYYSVTGKVSLEEEKDL
                     TASKIFPHLEELNKANEVAFYKERKEGAPIVKIVKPERIKSEYTAQADPIRDNTDLTT
                     VIRQPDLTKAETKTIVSPGSMTGVADLKIGDEKVSAEKTIEQTAKQDSKNEPLIITDK
                     SLPKTKETVSGVTDKKATDNTITGIKDNTEARSEPVSFSKQTTGLKQEDVVTQKPVMN
                     KPNPEPVASADPNKPEIISVKTNAGITKETPALEKKEIVTNPVTKTEQPVAAKKAETK
                     TKIPVSGPANPVTENKKTETTSTAPATNTNIAATTNREKKVTVATASNPVAKKPAEDV
                     IKKSAVIEGRKSEFSQEVNFKSDSLLLSLYDNGEIDGDTVSIYMNGDVIMPRQGLKSA
                     AIRKTIYLPAESEEFTLVMFAESLGKYPPNTGLLVIRDGDDVYNLRFSSDFQKSSGIL
                     FRRKK"
     gene            7800..8588
                     /locus_tag="PLO70_03795"
     CDS             7800..8588
                     /locus_tag="PLO70_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ73608.1"
                     /translation="MKQLLCLSCLLLFSLLSNGQTTQTDTVYSGTVKDKFVITIRKPA
                     GFSNSKKYHHVYMTDGSIGIGDYVLGRDNSWAASIPSNCVIIAISHIGDWNDKRPRDF
                     IPSDISRNTEENFGKADKFYGFMKNELIPTIENKIPNKRSRAFIGHSFGGLFCLYTLF
                     KEDKLFDRHFAISPSIWANYYELDKIEEKFSTTNKSLKANVTLYVGGLEFLNKVLYST
                     RSFYTTLIKRKYTGLAVKKDETGNANHFSIRKPAIDRIFESLKD"
     gene            8614..9654
                     /gene="selD"
                     /locus_tag="PLO70_03800"
     CDS             8614..9654
                     /gene="selD"
                     /locus_tag="PLO70_03800"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQZ73609.1"
                     /translation="MTQELIKLTQYSHGAGCGCKIAPKVLDEILKSSLALPDNDKLLV
                     GNHSKDDAAVYDLGYGSAIISTTDFFMPIVDDPFDFGRIAAANSISDVYAMGGKPMMA
                     VAILGWPVEKIPVEIAQKVIEGGRSICREAGIPLAGGHSIDSTEPIFGLAVTGIVPIE
                     NLKKNNTAQEGDYLFLTKPLGVGILSTAQKRGLLKEEHLPVMIEQMTTLNKIGEELGK
                     IKGVTAMTDITGFGLLGHLIEMAEGSGLSAEIYYDKLPIAAGVKEYIGQRIFPDATTR
                     NWSSYSDKVKFEKGVNVMEAFTLLPDPQTNGGLLISVKEESLAEVKKAVGEDLIPVGK
                     MLAAAEKVVYVK"
     gene            9758..9925
                     /locus_tag="PLO70_03805"
     CDS             9758..9925
                     /locus_tag="PLO70_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73610.1"
                     /translation="MKFTDNETLNNTLTFNACQADSLIEFLFNLPADFDNLTEDDLKY
                     KELEQLKLPQT"
     gene            complement(10007..11125)
                     /locus_tag="PLO70_03810"
     CDS             complement(10007..11125)
                     /locus_tag="PLO70_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQZ73611.1"
                     /translation="MKKNKILLCVCMLIYSGMFAQTKTDSTILLSSKERSIVLISAYT
                     AQGNMPKLQVALNDGLNEGLTISEIKEMLVQLYAYAGFPRSLNALNNLMAVLEERKKK
                     SINDAAGREPSPYPARKTMLQAGTENQTKLIGIKIGGGVYEFAPAIDQFLKEHLFGAI
                     FGRDNLDWKTREIITIAALAAMEGAEPQLRSHFGVGMYNGITKNQLAELVTIIEKEVS
                     PKRGMVSGQVLQAVINQKPYTPVTMPDEAVFPIGQKIDNNNFSGTAWLKQLILSDSSN
                     QTAVGNVTFEPGARTNWHLHPGGQILLATGGLGYYQEKGSPKRLLRKGDVVKCPPNVP
                     HWHGASHDQPFIQIAITNNQNGVTVWMQPVTDNEYNSN"
     gene            complement(11145..12197)
                     /locus_tag="PLO70_03815"
     CDS             complement(11145..12197)
                     /locus_tag="PLO70_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ73612.1"
                     /translation="MKTYLKLHLFSILFMLTLTTISAQTKTPNPFGLVYGAALTENVP
                     GKVNIHPVSYKLNGIVITANVYTPANYDSTKKYPAVVVAHPNGGIKEQVAGLYAQRLA
                     EQGYITIAADAAYQGASGGSPRNVDKPANRIEDIHGMADYITQYSGVDANRLGLLGIC
                     GGGGYALKAAQGDKRFVAVATLSMFNSGLVRRNGFQNSQLATIQQRLKQASDARAKEA
                     AGGEVIYAGIANPTDEEIAKIPTDLYREGYVYYYRTHTHPNSTFKYTMSSLLDLMTFD
                     AATNMDLIDQPLLMMAGSKADTKYMTDEAFPKAINAKNKDLFLIDGATHIQTYWKPDH
                     VDQATKKLTSFFGENL"
     gene            complement(12310..13395)
                     /locus_tag="PLO70_03820"
     CDS             complement(12310..13395)
                     /locus_tag="PLO70_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ73613.1"
                     /translation="MTRPNKTHVLLSAIVSLIVMSCSTNRNSVGSKYITIREQGSFAA
                     GGTVIANPGVFDPYKPTAAGQTLRGDHAYVFYQIPVKARKYPLVMWHGIGQFSKTWET
                     TPDGREGFQNIFLRRRFPVYLVDQPRRGNAARSIVEGNITPTPDEQGWFGTFRIGIWP
                     DYFEGVQFARDSTTLNQYFRQMVPNVGPIDINVNAEAVSALFNKIGAGILVTHSHSGG
                     MGWRTAIKNQNIKAIVSYEPGSGFLFPEGEVPAAMPSAGGTLEAVAVPMSDFMKLTKI
                     PIVIYYGDNIAKEPTNAPGPDGWRVRLAMARKWRDAVNKYGGDVTVVHLPEAGLKGNT
                     HFPFSDLNNIEVADLMSGWLKEKQLDQ"
     gene            complement(13422..14552)
                     /locus_tag="PLO70_03825"
     CDS             complement(13422..14552)
                     /locus_tag="PLO70_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQZ73614.1"
                     /translation="MKNSINKKAGNGINRRDLFKTGLVLAGGALLPSACTTSAGSVAG
                     KSNSSKQQVTTRRKLGGTLEVSSIGLGVQNMPRTYQTTIPSRPEMINIIRTAFDNGVT
                     LFDTAEVYGPLESERILGEAVAPFRNKIVIETKFFRSRPEQLKEVVEGMLTRLRTDRI
                     DLLYQHRVDRAVPIEDVVGAIKDLISQGKVLHYGLSEPGIQTIRRAHAVHPVTAIQNE
                     YSLLWRGPETEVIPLCEELGIGFVPWSPLGVGFLTGAIDANTRFAQGDIRGGEGRFSP
                     ENLPNNLALVELIKKWAIQKSATPAQISLSWLLAQKPWIVPIPGTTQMAHMIENNGAD
                     HLRFTADEWISFNADLAKIEIKGLRLPQGVLNMSGVEAPPKK"
     gene            complement(14593..15084)
                     /locus_tag="PLO70_03830"
     CDS             complement(14593..15084)
                     /locus_tag="PLO70_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ73615.1"
                     /translation="MKSFIPGLCFLLMCAQWCSAQSNQDSISKKRYTKAEQEIISLSK
                     QKWQWMADRNVDSLNVLFDEKSMFVHMGGSWGKAREIDVIKSGSIWYKKAEVYSVIVN
                     TIGNTVILLNDIDLVAVVGGNEVVNPFMVTEVYIKENGKWTMGSLTFSKLMRPVKMTS
                     NNK"
     gene            15305..16201
                     /locus_tag="PLO70_03835"
     CDS             15305..16201
                     /locus_tag="PLO70_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ73616.1"
                     /translation="MNNMRRFETINEYNVFNNNETRHPLVSVVDLSKAAPRQGSRMYF
                     GFYTIFLKDVKCGDLVYGRHTYDYQEGTLVFLAPGQVAGVNSNGETYQPKGYALVFHA
                     DFIHGTSLGRHIQEYSFFGYKSNEALHLSERERKIVLDCFSKIEYELEHAIDKHSKKL
                     IADNIELFLNYCVRFYDRQFITRDKPHKGILERFETLLNEYYQGDKPQISGLPSVAYC
                     AGELNLSSGYFGDLIKKETGKTAQEYIQSKVIDVAKEQIFDHSKSINQIAYDLGFKYP
                     QHFTRLFKQRVGVSPNEYRNLN"
     gene            complement(16394..17311)
                     /locus_tag="PLO70_03840"
     CDS             complement(16394..17311)
                     /locus_tag="PLO70_03840"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HQZ73617.1"
                     /translation="MPTSSTILMIRPAAFGFNKETAGDNFFQQNTGVSNETLQLNALA
                     EFDNMVHQLRNHAINVMVIDDTKEPAKPDAIFPNNWLSTSSAGVVSVFPMYAPNRRNE
                     KRDDILKQLAKEFVVKEVQDWSEYEAEGRFLEGTGSMVMDHDNKMIFAAVSERTNLSV
                     LEKYAATNGYQAIVFLATDKNGYPVYHTNVVMALGSGFCILCEEAIEEEWELIAVRQL
                     LESTGHTIIPITREQMHSFAGNMLEVKNSKGENILILSQTAFDSLRKEQKKMLEAYAE
                     LLPIAVPTIEQVEGGSVRCMMAEIFLQRK"
     gene            complement(17398..17634)
                     /locus_tag="PLO70_03845"
     CDS             complement(17398..17634)
                     /locus_tag="PLO70_03845"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQZ73618.1"
                     /translation="MWEEKDNKLYKKFQFKNFSEAFAFMTRVALEAEKMDHHPLWTNV
                     WNTVEVWLSTHDAGNMVTDKDHKLAKKIDAILER"
     gene            complement(17705..18151)
                     /locus_tag="PLO70_03850"
     CDS             complement(17705..18151)
                     /locus_tag="PLO70_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HQZ73619.1"
                     /translation="MAERSNKLFLDNKELTEGFFEDIRLLGIMAPIKDYQFCWHLNNN
                     IGLDFRINEIEIKLIKKKRDYFFSVYEYKVPTGTLEHYIYNNQFDGEYLLPEFKHLDF
                     LWLMKGDEVNDETMQQTIQIIRSINSVQLVAELSIDQVKNKENLVF"
     gene            complement(18179..19831)
                     /locus_tag="PLO70_03855"
     CDS             complement(18179..19831)
                     /locus_tag="PLO70_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQZ73620.1"
                     /translation="MISSFSRIIVVVVLVFAACSPAKKVTKADVQTLENLKLHIQYLA
                     DDKLEGRRTGTNGEKMAMEYISNQFKSIGLLPKGSKGYYQPFEINEGKQIGDSTKLII
                     NGNNLVAGKDFFPFPFSLNQKMEALPSISVQEADMPWFFDLKEQLEENKANPHFDLAN
                     YIYTNSKKAKDRGASAVILYNTSSIDDKLTFNGKDKSEQLTIPVLYVTRKEAQKYFKD
                     PSATIDIKFTTALSEKKRTGHNVVGYIDNGAATTVVLGAHFDHLGYGEDGNSRNTSTE
                     RIIHNGADDNASGTAALIELAKKMKQSKANNNNYLFIAFSGEELGLYGSKYFTENPTI
                     DLKTANYMINMDMVGRLSDSTKSVTIGGYGTSPTWSSVISMAPGNSITVKIDSSGTGP
                     SDHTSFYRKDIPVLFYFTGLHTDYHKPSDDADKINYNGIASILQHTTGLVESLNSSSK
                     LSFLKTRETQTTTSARFSVTMGVMPDYTYSGTGLRADGVSEGKPAQKAGLKAGDIILQ
                     IGDYKITSMETYMQTLGKFKKGDKTKVIYKRGNDTLETTVEF"
     gene            20007..22124
                     /gene="feoB"
                     /locus_tag="PLO70_03860"
     CDS             20007..22124
                     /gene="feoB"
                     /locus_tag="PLO70_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQZ73621.1"
                     /translation="MKEKRLHIALVGNPNSGKTSLFNCLTGLNQQVGNFPGVTVDKKT
                     GTTSLGSILAEVIDLPGSYSLYPKRLDEWVSYKVLINQDKDINADVVVAVADASNLKR
                     NLLFCTQIIDLKVPVVIALTMMDLAKRKGIKIDIAALERELGVPVVAVNPRKNKGIVE
                     LKKAIGLTAQQVYKVPARDFIDNKSLAETSIENIKQHFPELSDYKAIHYLINHESFDF
                     NASLQETIENIEIKNKFNPTKTQAEEILQRYSRIRQVMQQAVSEPDPLQKTLFTEKLD
                     NVLLHRRWGYLILLAVLFLLFQSVFWLAQHPMDWIELGFAKLSESLSSSLPDSRWTDL
                     LINGVIAGLSGILMFVPQIMILFGLITLLEDSGYMARISFLSDRLMRSVGLNGKSVMP
                     MISGFACAVPAIMSARNIENRKERLLTILITPLMSCSARLPVYTILIGLVIPQTNLLG
                     FLSVQGLVMMGLYLLGLVMALIVSYVAKWFINIREKSFFILELPTYRSPRWNNVLPTM
                     INKARIFVMDAGKIIMIISLVLWGLSSFGPGNTMQNLTQQYDQMKTQPGAAIAQLDKE
                     YHTAKLENSYAGIIGKSLEPAIAPLGYDWKIGIALVTSFAAREVFVGTMATLYSVGDT
                     DEGSLLLKEKMKAAVKPDGTPVFTLATGLSLMIFYVFAMQCMSTLAVVKRETRSWKWP
                     VIQLIYMTALAYLMSWVVYQIFK"
     gene            complement(22127..23029)
                     /locus_tag="PLO70_03865"
     CDS             complement(22127..23029)
                     /locus_tag="PLO70_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HQZ73622.1"
                     /translation="MNKKSVLLILFVFFAGRDTLFAQQTDKKLKAKLEEVIRGFKGDI
                     GIYVKNLRTGKVVAINADSVFPTASIVKVPILLGIMDKIQRSELHYDSSLVYKDSLLY
                     AGSDILGSFKTDEKIALKKVIMLMLTTSDNTASLWLQSLAGTGTGINEIMDSLGLKYT
                     RVNSRTPGREVNRAQYGWGQTSPSEMAMLFEMIYRNKVISPAACERMMRCLGRNFWDA
                     EALSQIPPYIEVFSKNGAVNASRSEVLLVNAIHNPYIFSIFTKNNKDISWGETNEAWV
                     LTRKISRILWEYFEPKDKWEPGLK"
     gene            complement(23032..24357)
                     /locus_tag="PLO70_03870"
     CDS             complement(23032..24357)
                     /locus_tag="PLO70_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQZ73623.1"
                     /translation="MKQKLSLLLFLIPVILFAQKNGPDIAEKISIATDKIEAKCNNWR
                     RDFHQHPELGNSEFRTAKIIADHLRSLGIEVKENIGKTGVVGILKGAKPGPCIGLRAD
                     MDALPVVERVNLPFASKAQSTYNGQSVGVMHACGHDAHVAILMSVAEILAGMKNELTG
                     TIKFIFQPAEEGPPEGEEGGAPLMIKDGVLENPKVDVMFGLHINSQTEAGKIKYRVGG
                     IMAASDWFKIKVKGKQTHGSQPWAGIDPVVVSAQIINGLQTIVSRQTELTKNPAVISV
                     SVINGGVRSNIIPEEVNMLGTIRTLDTGMQRIIHEKIILTASKIAESTGATAETVIDK
                     KTLVTYNEPALTKKMIPSFIKAAGADNVVETEAITGAEDFSFFAEKVPSLFFFIGGME
                     KGKDAKKAFPHHTPDFYIDESGMKTGIKAFCHMVLDYMNMSKAQTKKGF"
     gene            24447..25685
                     /locus_tag="PLO70_03875"
     CDS             24447..25685
                     /locus_tag="PLO70_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQZ73624.1"
                     /translation="MLLRHASFLRAVFIHSVTAFGGPQAHIGMMMKTFVRNKPYVSEK
                     ELMEYNAFCQLLPGASSTQTLTLIGYKRGGVPLAVLTLLIWILPACILMGAFSFLLQY
                     IDKKELGTDIFKFIPPMAAGFLGYAVIHIFPHAIRNTITWIIMLCSVAATYFLFGRTW
                     VIPALIIAGGIATNFSLKRIPQVEQKPRKIRWWNFWLFGIIFLLAGITSELARKNDWP
                     NRKPINLFENTYRMGSLVFGGGQVLMPMMYEQWSIRPEAVRSKNPNAVQIDQDKLYTG
                     MGIVQAVPGPVFSIASFAGGMALKDMGTEMQVLGCIIGMIGIFLPSALLVLFFFPIWN
                     NLKKYAAVYRSIEGINAVVVGVMIASSFYIMRDISFIEPKTVSLVNILVILGTIILLN
                     FTRILPPFIVITCLLLGYFL"
     gene            complement(25673..26245)
                     /gene="nadD"
                     /locus_tag="PLO70_03880"
     CDS             complement(25673..26245)
                     /gene="nadD"
                     /locus_tag="PLO70_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQZ73625.1"
                     /translation="MKIGLYFGSFNPVHVAHLIIANHILNETDIEKVWFVVSPQNPFK
                     TENSLLNEYHRLHLVKLATENDNRIRASDIEFTLPKPSYTVNTLAYLSEKHPEHEFSI
                     IMGSDSFQNLPKWKNYGVIVKNYPIYVYLRPGFEIEDKLNADVHFVDAPLLQLSATQI
                     RKYAKEGKSLRYLVPDKVLEEIERGGYYKK"
     gene            complement(26263..26493)
                     /locus_tag="PLO70_03885"
     CDS             complement(26263..26493)
                     /locus_tag="PLO70_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQZ73626.1"
                     /translation="MIARLSELKPGQQGIIREFIDNDIFLKLMEMGCVPGERILLEQI
                     APLGDPISVNVAGYHLSLRLNEAEHILVEINN"
     gene            26558..26989
                     /gene="ybeY"
                     /locus_tag="PLO70_03890"
     CDS             26558..26989
                     /gene="ybeY"
                     /locus_tag="PLO70_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQZ73627.1"
                     /translation="MPSKSKVCFFYEYKKFSLENRGALKFFIESIFKKEKRPLSSINY
                     IFCSDKRLLEINQQFLKHDYYTDIITFDLSDTNSIHAEVYISIERVKENAKSLGSSFK
                     AELHRVIFHGALHLCGFRDKTKTEKEHMRQKEDVYLSRYFK"
     gene            27111..28973
                     /gene="mnmG"
                     /locus_tag="PLO70_03895"
     CDS             27111..28973
                     /gene="mnmG"
                     /locus_tag="PLO70_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQZ73628.1"
                     /translation="MFSQYDVIVVGAGHAGCEAAAAAANLGSRTLLVTMNMQTIAQMS
                     CNPAMGGIAKGQIVREIDAMGGYSGIVTDLSTIQFRMLNRSKGPAMWSPRAQNDRMLF
                     HLKWREKLEQTMNLDFYQDMVKGLLVKDGKCYGVVTGMGHEIKAKSVVLTNGTFLNGI
                     VHIGEKNFGGGRMAEKAATGITEQLVSLGFESDRLKTGTPPRIDGRSLDYSKMEEQPG
                     DEEIVGFSFLETEKPKQQRSCWITYTNQTVHDILKTGFDRSPMFAGRIDGVGPRYCPS
                     IEDKINRFAERDRHQLFIEPEGWNTVEIYVNGFSTSLPEETQYEALRNIVGFEKVKIF
                     RPGYAIEYDFFPPTQLRYSLETKLIDNLFFAGQINGTTGYEEAACQGLMAGINAHQKS
                     HHQEPFILKRSEAYIGVLIDDLISKGTKEPYRMFTSRAEFRTLLRQDNADLRLTELSY
                     RLGLASQQRMDKVIEKKASIEKVKTVLKDLAVEPNEINTWFQKIGSSPISEKQKAQKI
                     LLRPDVELDEFAKAFPKLELALQGLSKESVEQASIQVKYDVYIEKEKELVQRMSQLEE
                     LEIPETFDYKKISSLGNEAREKLTKIKPRTLGQASRISGINPSDVQILMVFMGR"
     gene            29065..30408
                     /locus_tag="PLO70_03900"
     CDS             29065..30408
                     /locus_tag="PLO70_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73629.1"
                     /translation="MKKNVLCFIAVMITAFVIDNEATAQQYKLRQSTSMMGMKTESTI
                     YVKGMRKRTESAGMMGMPAPPITIEQCDLQRTIKINDKKKLYYIESFSKENEEVVDED
                     AKPAAPVKNKPVTPAPKEKTTPQKGGVITMWYNITDTGERKNIYGFNARHVWTSQKIK
                     PSPDACTMKDSILIKTDGWYIDLPLFNCPVRYRPVKASMAPTDRPQPDCKDRFVTRRS
                     GKGKLGFPLTETTTIKMGGGNAQMSEFQTSIETLELSTVKLDSMLFEIPPGYTETLNE
                     DDLQEKFDAGDMMNQAMKEYKNKAETKPALTEQKKDGIIRIGVYEPRGDEQLQASVLQ
                     QHLVNTLTDGTVEAISIISEDDAKKYNCDYTLASDFIKIKQGSKVGGLLKAIKNTDPN
                     AASSFTIEAAMTLVNLSDGSTRLQPKINGKYDGKADEAAKKALDDGSRQVLRALK"
     gene            complement(30501..31406)
                     /locus_tag="PLO70_03905"
     CDS             complement(30501..31406)
                     /locus_tag="PLO70_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HQZ73630.1"
                     /translation="MSRFAILKQIMLQHRYRLLFTYVLFSLEMLGNLLRPFFLGLAVD
                     DLIKGSYKGLVLLSAVHLGWLIIGTIRHRLDTRTYSAIYTSLVTKFLARRYNKSDISK
                     LSAHSTLAREFVDFLEFDLAYVVEAFYSIVGSLLLLFFYDTSVVMVCLGILLPVTGLS
                     YFYGKKMRNLNKQKNDELENQVDIISTGNNHLIRQHYDNLRKWQIRISDQEAWNFGIM
                     EIMVMVVIGLALLITNKTTGAGIEAGSLIGIYSYIQRFVSGLDTIPYTVQRLSSLNDI
                     TRRIELQEEDLRMPDPVNLKVKVVA"
     gene            complement(31504..32940)
                     /locus_tag="PLO70_03910"
     CDS             complement(31504..32940)
                     /locus_tag="PLO70_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQZ73631.1"
                     /translation="MKVCVLQPDYSTTGVDYKNYDPPRDLSVLIPEAEVHTIFLNKLT
                     TYKQLKQLSKDGYDIFVNLCEGYLEWEVPSIDVIYTLEILNLPFTGPTTKLYDPPKEL
                     MKYLAYCEGVKTPGYVIINELNDVDDAVKHLLFPLFVKPAKAGDSLGVDEQSLVHNKI
                     ELQQKCISILDEYGPLLVEEYVAGREFTVLVAANTDEHSCTVYRPVEYLFPEGREFKT
                     YALKTSELHPDCNVPCNDELLDKQLRNATEKIFKGFNGVGYARLDFRVNDKNEIYFLE
                     INFTCSVFYKDGYEGSADFILKYDGIGQAGFLRHMIAEGISRHKRKMKPFVMKGNSIA
                     GYGIYARRDIKAGEVIFKGEGRAQRIITKRFVERNWNEDEKLHFRRYAYPISDEVFIL
                     WDDDPSEWAPQNHCCDPNTSFDGLNVLAIKNISKGHELTLDYADFLDENMEPFDCQCG
                     SISCRGKISGHTGNSLNLREQNLLRLND"
     gene            complement(33008..34168)
                     /locus_tag="PLO70_03915"
     CDS             complement(33008..34168)
                     /locus_tag="PLO70_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73632.1"
                     /translation="MKTAEASRLLKPYFIWVLAPHLESEDPNLKYYYDFTQSIAEYTK
                     VFEELKAEWKWQPVTVENFKIVIKSIAESKNGKTPLILNLCDGDEVNGTPGVSVIHEL
                     EKYGLIYTGSDAHFYAITTSKIPMKRAFDKAGVSSANWRVITDKKGSTKGIAKRVGTP
                     IIIKPAVSGGSMGVSVKNVVFTEEELTERVEEIKKGYRGWNLLADGLFVEQFITGPEF
                     TTFITGSADDPENCKVYEPVERVFHTSLPDSEKFLSFDRLWEIYEDETPMPREENFYE
                     YQRVKPDLVAALKTLSLDAYKACGGKGYTRIDIRQDSSTGKLYMLEANAQCGLSEDEN
                     YTSIGAILKASNVSFTEVISEILKDALRRSVVKQVPVKKAKVAKIKKTIVVK"
     gene            complement(34175..34681)
                     /locus_tag="PLO70_03920"
     CDS             complement(34175..34681)
                     /locus_tag="PLO70_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQZ73633.1"
                     /translation="METLTNPAYRIISNHVIAEVRQKISNDQNALFALRPYQPGEVIA
                     DFSAGTIAAEPTYLTVQVGVGKHITLQPEFLQYINHSCSPNVFFDTSAMQLVALRELD
                     VEEEMTFFYPSTEWEMTQSFSCYCGSPACIGEIRGAAFLSKDIAAQYRFTDFIQQQLA
                     KKAARKVA"
     gene            34929..36386
                     /locus_tag="PLO70_03925"
     CDS             34929..36386
                     /locus_tag="PLO70_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73634.1"
                     /translation="MMTRLVYLCCYFLLGYADSKAQCSSAINSFPYSESFETTDGSWI
                     SGGVGNDWAWGVPSKPVISAAGGGVKCWVVGGLTGSSYTNSEASWLQSPCFDFTNLIS
                     PYIEFKIFWESEQRFDGASFQYSTDNGITWQTMGSSANTVNCYNENWYNNSSITYLSP
                     LASNKMGWSGNIQTSSGSCAGGAGSGKWLTAKQAMGFLEGKPNVIFRFIFGAGSICNN
                     YDGFAIDDILIKQAPAYYASFTYTCDGEGIVSFTNTSDACVTIQNWDFGDPASGASNS
                     SDIPSPTHKYSAPGKYTVKLHASFPGSSISTATQTITIPDVRVTQLSPADCQTNSGGS
                     IIANVSGVNNPVNYSWNTVPPQITQIASNLSAGLYTVTITGTDVCPTKATGELVTDFS
                     CPGIFFPSGFTPNGDGKNDSFGPIGGLGVISNYQFSIYNRWGERVFYSTNPFEKWNGL
                     SKGIKADGNSFAWYAEFTLPGKPKESRKGIVTLIR"
     gene            complement(36375..38951)
                     /locus_tag="PLO70_03930"
     CDS             complement(36375..38951)
                     /locus_tag="PLO70_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQZ73635.1"
                     /translation="MQRQRLAQCSFSFYANDCMMKYDTENEMFQLAIQLVNQSNRNIF
                     LTGKAGTGKTTFLKYIRENCPKQLAVVAPTGVAAINAGGVTIHSFFQLPLAPFIPATN
                     GTGFQSSNPEISNKHSLLSRLRFNSDKKKVLQQLELLVIDEISMVRCDTLDAIDTVLR
                     HIRQRPDERFGGVQLLFIGDMLQLPPVIKEQEWNLLADYYSGHYFFDSKVLQEDPPVY
                     IEFNKIYRQSDQQFIDLLNQVRNNELDKEGMSILESRFQPSFRRSKQDGYIVLTTHNN
                     KASEINANELSSLSGKLYSYDAEVLDEFSDRAYPADEVLHLKVGAQVMFIRNDAAEKG
                     KRYFNGKIGTVAKLEADKIVVQCDDDPEIEVQKEKWENIRYTLNKTTRQLDADVLGSF
                     TQFPLRLAWAITIHKSQGLTFEKAIIDAGAAFAPGQVYVALSRCSNLEGMVLQSHISS
                     SSLFSDRRIVEFSQRSASGNQLQQELAIAKKNYQQSVLAGVFDFSNAIDGCKEIMDYL
                     LEHTSSFNPDSFGWTEELSEKLGGLQVTAKKFQTQLRALFQQPVEPEENIALQDRIKV
                     AAVYFNNEINRLLQFIHTSPVITDSRLNAKEYNESIREVFALLAMKKFLLEGYNGQLD
                     MEAFHRRKLQFVLPSFSINAYAGASQKRFDSPHPVLHQQLRKLRESICAKRDLPIYIV
                     AGSNTIDEMAMYLPQTLTELRKISGFGDTKIEQYGQQFLDIILQYTREHNLQSLIHEK
                     VSGKRKKELISPGKKKGDTHIETFRLYKEGQSIAAIAKTRKLAVSTIEGHLTTFVRRG
                     DIKISELVSREKLVLIESALEEFSGSSITPLKKRLGDDISFGEIRLVMAGLGISPEKS
                     PD"
     gene            complement(38976..39317)
                     /gene="ssb"
                     /locus_tag="PLO70_03935"
     CDS             complement(38976..39317)
                     /gene="ssb"
                     /locus_tag="PLO70_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQZ73636.1"
                     /translation="MYALKNKVQLIGNLGNAPEVKTLESGKKMARFSVATNENYRNAK
                     GEKVTETQWHNLVAWGKVAEIVEKFLTKGKEVAIEGKLVNRSYNDKEGNKKYVTEIQV
                     NELLMLGVKAE"
     gene            39539..39922
                     /locus_tag="PLO70_03940"
     CDS             39539..39922
                     /locus_tag="PLO70_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73637.1"
                     /translation="MIKEKTDKRTCLACGNVLKGRADKKFCNDYCRNNYNNEQKAKGG
                     QSPLVRNINNALLKNRKVLESILPEGEETAKANKDKLQRLGFQFKYLTHIYTTKTGKT
                     YYYCYDYGYLPLDNDWFLIVKKKEE"
     gene            39946..40872
                     /locus_tag="PLO70_03945"
     CDS             39946..40872
                     /locus_tag="PLO70_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQZ73638.1"
                     /translation="MSNPVICIGAALVDELFHATDNMLFATTNDATVTKTAGGVSRNI
                     SHQLALLGVPVQLISVFGDDSDGDWLKQVCINAGVKLDASITKKGLSGKYTGILNVDG
                     SLFSAFLTNAANHLITPEHLTGHIELLRTASFLLADANISVDTAEWLLAFSKETGIPF
                     VLEPVSVPPAKKFTNVNLDGLYMITPNEDELPVLCSEQASMTQMQIQQLLEKGVQNIW
                     LHNGKMGSALYNKEKAITLPAPSLEVVDCTGAGDGSLSGFILGKHLGKEDMDCLKLAH
                     TLSAEILQVNGAIATHLDQEKLLGLITKYYPS"
BASE COUNT        11932 a         8584 c         8243 g        12127 t
ORIGIN      
        1 tttaccccgt taatactcac ttcatccagc acatccacta catttttgta actagctccg
       61 gacccgggtt tgatcactaa cataaggcca ttgcgacctt cctttttatt gtaattgtcc
      121 aaccactgtt gttttgcaat aatgtttttt cgcaagccat tgtttccgga aaagccggtt
      181 tttatgatgg tatgctgtat aactgcatct tcccagcggc cgtgataaca ccaggctcta
      241 ttgtctttgt caagtaatac agtcaatgcg tttaattctc ccaactccat cggcacttta
      301 ctttctttcg gcataaacag atccatagct gttggcttgc tcaactcggt ggttattaca
      361 aaaaaactga tgagcaaaaa gccaagatca accatcgggg tcatatcaat tttcgggttg
      421 tgttttgcaa ctcccggcgc accaaccctg tttacttttt tgcctgaata agtatgaacc
      481 gtcgtattcc attgagccat aagtgctgtt ttgataacag acacaaaaag acattaattc
      541 cacaaaagaa tcattgttag ccctgatgac aatcatagct gcaaaaggga taacctggtt
      601 acctttaatt acaatttatt tgcaatgaaa acaatcatta tccccactga tttttcgcct
      661 attgccacca atgcattgca tttcgggatt gatatggcga aagctattaa tgccagtata
      721 ttactgttgc atgtatacca ggtgccggta agttttacag atacaccgat tgttttagtg
      781 tcgatagaag accttagaaa aggggctgag gaacaaatag agaagcttaa gaaagaagtg
      841 gagcatctta cgtctggttc ggtaaaagta tatacggaaa cccgaatggg taatgttact
      901 gatgaattag aaaccctgtg tagtaaaata aatccttttg ctgtagtaat gggaaccaaa
      961 ggctcttctg gtgttgagcg aattttgttt ggcagcaata cccttaatgc gatacgccac
     1021 cttaactggc cagtgatctg tgttccgcca ggaaaagctt ttggacacgg aataaagaaa
     1081 attggttttg cctgtgattt cagggatgtg gtgaagtcta cccctacaca tttcataaaa
     1141 gaatttgtga aggaatttaa tgccgaactg catgtattga atgtagacca tgataataaa
     1201 cacttcaaac ctgaaacccc tgagcaatcc atcctgctgc atacattact ggaagaggca
     1261 aacccttcct atcactttat tgaacatgca gacatagaag atggcataaa tgaatttgca
     1321 gaaaagaata acctggacct tatcattacg atccctaaaa agcataaact gttacaggga
     1381 atattcaagc cgagttctac caagcagttg gtttaccagg ctcatgtacc ggtgatgtgt
     1441 gtacatgaat agatttgatt aaaaaagaga aaggagataa agaattaatt ctttatctcg
     1501 ttttttatta gaacaatttt ttacgctgtt taaaattccg cacttcccgg cgttctggga
     1561 aaagctatca catcacggat attcgtcatg ccggtaacaa actggaccat acgttcaaaa
     1621 cccaatccaa agcctgcatg cggaacggtt ccgaaacgtc ttgtatccaa ataccaccac
     1681 agatcttcga caggaatatg catttctttc atccgctgtt ccagcttatc ttgtctttct
     1741 tctctttgag agccaccaac tatctcccca atacccgggg ccaggatatc catagcagca
     1801 acggttttac catccgcatt ctgccgcata taaaacgctt tgatctcttt gggataattg
     1861 gaaagaataa ccggtttctt aaaatgcttt tctaccagat atctttcatg ttcactctgc
     1921 aggtccattc cccatccggt aatctcgtac tgaaacttct ttttcttgtt atgattactc
     1981 tctttcaaaa tatcaattgc ctcagtgtag gttatccgtt caaaattatt attcaataca
     2041 aattccagtt tcccgatcag ccccatttca cttcgcttat cctgcggcaa ttgtttttct
     2101 tcgtcagcca gccgttgtgc caggaattcc aggtcctccc ggttgttgtc tattgcatat
     2161 ttaataatgt atttgatgaa ctcttctgca agattcatat tgtcttccag atcatagaaa
     2221 gccatttctg gttctatcat ccagaactcg gccaggtgtc ttgcggtatt gctgttctct
     2281 gcacggaacg taggaccgaa tgtataaata tcaccaaaag cggtagcacc caattctcct
     2341 tctaattgtc cgcttacagt aaggttagta ctgcggccaa aaaaatcttc tttaaagttg
     2401 atggaaccat cttcattttt cggagcggta ccatcaatgg gcaacgtagt cacccggaac
     2461 atttcgcctg ccccttctgc atcactggca gtgataatag gagtgtgcat atacacaaac
     2521 ccttttttat tgaaaaattt atgtacagca aaagccagcg aatgacgaac acggaacacg
     2581 gaaccaaatg tgtttgtccg gaaacggaga tgtgcctttt cccttaaaaa ttccaaacta
     2641 tgttttttgg gttgcagcgg atatttttca gcatcgctgt caccgagtat ctctatggtg
     2701 tccgctttca gatccatttt ttgtccctta cctaatgatg gtacaattgt acctgttact
     2761 tttaatgatg ctgatgttgt gattctcttc agtatgtcgt cgttaaactt acccagttct
     2821 actaccacct gcagattatt attggtactg ccatcattca acgctataaa ctggttattg
     2881 cgaaaggttc gcacccatcc catgacaatt acttcctggc cggccggttc ctttgccagc
     2941 agttctttta tctttatcct tttgttgaac attttgaaat tttaagagtc gcaaaggtaa
     3001 gcttcgtcag ggaactgaac gatgaacgga atcccggtag tcaccggggt aaacgctgca
     3061 acatcagccc aaccagggtg ttaaagctgg taaaatccaa aaatttttga ttttcccgaa
     3121 ttagctgtat acttgcactg gaaagaggct gataaacaat agttctccca gacagttttt
     3181 ccattttacc cgcctgtttc attcaacaaa aactcaaaca ttttttcaga tttcaagatt
     3241 gttcactgcg agttgtgact aacatcaaga tttttatatg tacgatattc tccaactgaa
     3301 cgacatgctc gttcctgaac tgctggacat agccgaccag cttaaaattc ctaacgctaa
     3361 aaagctgagc aaacaggatt tggtttacaa aattcttgat agccaggcta ttgccggctc
     3421 caaaggtgcc aaacccgcag atgacggcaa acgtaagcgc atcatcaaaa ccagcaccag
     3481 caccggtagc gaagaagcag tagtggaaag tggtggcgag gaaaaaacaa ttatcaaaaa
     3541 acccattatg gctgttaaaa aagatgataa gaccccaata aaaagggctc gtaagaaacc
     3601 cgaagaaaaa gaaattactg ctgatgcaga tacagctgtg gtcgaaacac aaccaaaaga
     3661 gacagaggct gaattacaaa ctaaaaccga ccagcttgca caagctattc tcaatgaagg
     3721 caaccagcaa tctccggaaa acccatccgg tgccggacaa aataaattct atcccacccg
     3781 tcgtgaacaa cagcaacaat tcaatgtgga gtttgacggt gttattttag gtgagggtgt
     3841 actggaaatg atgcctgatg ggtatggatt tctccgctct tcagattata attatttatc
     3901 ctcccctgat gatgtatatg tttctccctc acagataaaa ttattcggat tgaaaaccgg
     3961 tgacactgtt catggtgcag tgcgtccgcc aaaagaaggg gagaagtatt tcgccttatt
     4021 aaaagtggat acaatcaatg gtaagagtcc ggaagaagta agagaccgtg tgccttttga
     4081 ttacctgact ccattgtttc cgtatgagaa actgaattta tttaccacac cttctgatta
     4141 ttcaacccgc atcatggacc tgttcacccc cattggtaaa ggtcagcggg gattgatagt
     4201 ggcacaacct aaaaccggta agacaatgct attgaaagca gtggccaacg ctattgctgc
     4261 caatcatcct gaatgttacc tgatggttgt tcttgttgac gaacgtccgg aagaggttac
     4321 tgatatggaa cgcagtgtaa gggcagaggt aattgcttcc acctttgatg aaccagcaga
     4381 gaaacacgtt aaagtttcaa cgatcgcttt gcaaaaagca aaacgcttag tggagtgcgg
     4441 acacgatgtg gtgatattac tggattctat tacccgtttg gcaagagccc ataataccgt
     4501 ggcaccatcc tcgggaaaag ttttatccgg tggtgtggaa gcgaatgcca tgcagaaacc
     4561 aaaacaattc tttggcgctg cccgtaaaat tgaacacggc ggttccttaa ctatcattgc
     4621 aacagctttg gttgataccg gcagtaaaat ggacgaggtg atctttgaag aattcaaagg
     4681 aaccggtaac atggaattac aactggatcg tcgtttggct aacaaacgca tttatcctgc
     4741 tattgacctg gttgcctctt ccacccgtcg tgatgatctc ttattggata gggacgttct
     4801 gcaaagaatg aatctgctcc gtgtttatct tactgatatg aatgcagaag aagccatgcg
     4861 agaactgcaa aacaggatga agggaacaaa gaataatgaa gaatttttgg ccagtatgaa
     4921 caggtaatgt gacaattagc caatttgaaa atttgaaaat ggctgcttta taatgaggcg
     4981 gcctttttta tttttgataa cccaaatggg agttgaaaaa atacatattg aacaattcct
     5041 ggagctggca aaacattatc ctgtttttga tgttcgcagc ccgggggaat acatgcatgc
     5101 acatattccg ggtgcctgtt cattgccatt atttacagat gaagaaagaa aagtagtggg
     5161 cactacctac aaacaacaaa gccgggaaca ggctataaaa attggattgg attattttgg
     5221 gccaaagatg agaaagatga tagaggaggt cgagtccata tgcagtagcc ggaattcact
     5281 gctcacaacc caggactcac gaatcattct tgtccattgc tggcggggtg gtatgcgtag
     5341 cggggctatt tcctggttac tggatctgta tggctttaaa gtttataccc tcataggtgg
     5401 gtataaaaaa ttccgcaact atgtgctgga cactttcaag ttaccttttc atctcaatat
     5461 cctgggtgga tataccggtt ccggtaaaac tgaattatta aaagcgctga aagaaagggg
     5521 ggaaagggta atcgacctgg aagatatagc caagcacaag ggttcggctt ttggcagtat
     5581 tggaatgccg caacagccgg gacaagagat gtttgaaaat atactggctc aaagcctgca
     5641 tgaggtgaga ggtaagctat cagaaaccgg aaagacccct tcagacttca tggctcatcc
     5701 ctccggcatc tggttagaag atgaatccca gcggatcgga catgtgaata ttccgggcga
     5761 cttttggcaa aatatgcggc aatctccatt gtactttcta gacatccctt ttgaagaacg
     5821 gctgaagcat attgtgcagg aatatgggca gttggaccct gaaaaagttg cagatgccat
     5881 tggacgaatt agccaaaaat tgggcaattt gaatgcaaaa acagccattt tattactaaa
     5941 agagggcaaa atcaccgaaa gtttcaatat tttgctcaac tattatgaca agttttattt
     6001 taagtcttta cacaatcggg aaggggtgaa ttcgttatta caggtgatag aatgtaaatc
     6061 cataacccct gagaatgccg ataaacttat acgccaggct aattaccacg ccgaaaggtc
     6121 ttagcctttc aatcctattt cttttattcc cgattttttt tcatgcacag tcattaacgg
     6181 gcctgtgggt aggagcactt agcaatgaca gcaccacgac caggaaagat caatcatttg
     6241 aaatagccct gacagagtat aagggtaaag tgtatggcta ttcccgcagt gaatttatcg
     6301 ttaatgatac cctttattac attgttaaaa gagtaaaagg aataattgaa ggtgatgcct
     6361 gcgaagtgtc cgacgaggag atcattgcct ataacttccg ggggaagctt gacaagggta
     6421 taaaagttac cagcactttt cgccggaata aaaatgacag cacctggtat cttgacggca
     6481 cctggaagac aaatgcaacc cggaaatact attcggttac cggcaaagta agcctggaag
     6541 aagaaaaaga cctgacagcc tctaaaatat ttccccacct cgaagagtta aacaaggcaa
     6601 atgaagtggc tttttataaa gaaagaaaag aaggtgctcc cattgttaag attgtaaaac
     6661 ccgaaagaat aaaatctgaa tacactgcgc aggcagaccc gattcgggat aatacagact
     6721 taacaacagt aattcgtcaa ccggatttaa ctaaggctga aacaaaaact attgtaagtc
     6781 cgggctcaat gaccggtgta gctgatttga aaataggaga tgagaaagtg tccgctgaga
     6841 aaacaattga acaaacagca aaacaagact caaagaatga accactaatc attacagata
     6901 aatcattacc gaaaactaaa gaaactgtta gtggcgtaac tgataaaaaa gcgactgaca
     6961 atacaattac cgggataaaa gacaatactg aagccaggtc agaacctgtt tcgttttcaa
     7021 aacaaacaac aggattaaaa caggaggatg ttgttacaca aaagcctgta atgaataaac
     7081 caaaccctga acccgttgca agtgctgatc cgaataaacc agagataata tctgttaaaa
     7141 caaatgctgg tattaccaaa gaaactcctg ccctggaaaa gaaagagata gtaacaaacc
     7201 ctgttacaaa aacagagcaa cccgtagctg caaaaaaagc tgaaacaaaa acaaagatcc
     7261 cggtatcggg cccggcaaac ccagtaacag aaaacaagaa aacagaaact acttctacag
     7321 ctccggcaac taataccaat attgctgcaa ctaccaatag ggagaagaaa gtaactgttg
     7381 caacagcatc gaatccggtt gccaaaaaac ctgctgaaga tgttataaaa aaatcagctg
     7441 ttatcgaagg ccgtaaatct gaattttcgc aggaagtgaa tttcaaatca gacagtctcc
     7501 tgctttcatt gtacgataat ggcgagattg atggtgatac tgtcagtatt tatatgaatg
     7561 gagatgtgat catgccccgg cagggattaa aaagtgccgc tatcaggaaa acaatatacc
     7621 tgccggcaga aagcgaagaa ttcaccctgg taatgtttgc cgaaagtctc ggtaaatatc
     7681 cgcctaatac aggattgtta gttatccggg atggagatga tgtatataat cttcgattca
     7741 gctccgactt tcagaaaagc tcaggtatct tatttcgcag aaaaaagtaa tttaacagga
     7801 tgaagcaact gttatgctta tcctgcctgt tattattttc attgctaagt aatgggcaaa
     7861 ctacacaaac ggatactgtt tattccggta ccgttaaaga caagttcgtc atcactatcc
     7921 gtaaaccggc ggggttcagt aattcaaaaa aatatcacca tgtgtatatg accgatggtt
     7981 cgatcggcat tggtgattat gtgttaggcc gcgacaattc atgggccgct tcaattcctt
     8041 ctaactgtgt catcattgct atctctcata ttggcgactg gaatgataaa cgaccaaggg
     8101 attttattcc ttcggacatc agcaggaata cagaagaaaa ttttggaaaa gcagataagt
     8161 tctacgggtt catgaaaaat gaactgatcc cgacaattga aaataaaata ccgaataaga
     8221 gaagcagggc ttttattggc cattcatttg gcggactatt ttgcctgtat accttattta
     8281 aagaagataa attgtttgac aggcattttg ctatcagccc ttccatatgg gccaattatt
     8341 atgagctgga caaaatcgag gaaaagtttt caacaaccaa caaatcatta aaggccaacg
     8401 taaccctgta tgtgggagga cttgagtttt tgaacaaggt attatactct accaggagtt
     8461 tttatactac cctaatcaaa agaaaataca caggcctggc tgtcaaaaaa gatgaaactg
     8521 gcaatgccaa ccatttcagt atccgcaaac cggcaataga cagaatattt gagtcgctga
     8581 aagactaact ttggcggttc aatatttaca ggaatgaccc aggaactgat caaacttact
     8641 caatactctc atggggccgg ctgtggctgc aagattgctc ccaaagtgct ggatgaaata
     8701 ttaaaaagca gtcttgcttt acccgataat gataaactgc tcgtcggaaa tcacagtaag
     8761 gacgatgccg ctgtatatga tttgggttat ggatccgcca ttatttcaac cacggatttc
     8821 tttatgccca tcgtggatga tccctttgat tttggcagaa tagctgctgc taattctatc
     8881 agcgatgtat atgctatggg tggaaaacca atgatggcgg tagctatcct tggatggccg
     8941 gttgaaaaaa taccggtaga aatagcacag aaagtaattg aaggaggaag aagtatttgc
     9001 agggaagccg gcattcccct ggcaggcggg cacagcattg attctactga acccattttt
     9061 ggtttagccg ttactgggat tgtacctatt gaaaatttaa agaagaataa cactgcacag
     9121 gaaggtgact atttattcct gaccaaacct ttgggtgtgg gcattttatc aactgcacaa
     9181 aaaagagggc tgctgaaaga agaacacctg ccggtaatga ttgagcaaat gacaacattg
     9241 aataagattg gtgaggagtt gggtaaaata aaaggtgtaa cagccatgac cgatataacc
     9301 ggctttggcc tgttaggaca tttgatagaa atggctgaag gcagcgggtt aagtgccgag
     9361 atctattatg ataagctacc aatagccgcc ggagtgaaag aatatattgg ccagcgcatt
     9421 ttcccggatg ccaccacccg caactggagc agctacagcg ataaagtaaa atttgaaaaa
     9481 ggggtgaatg tgatggaggc ttttacccta ttacctgatc cgcaaacaaa cggagggttg
     9541 ctgattagtg tgaaggaaga aagtctggcg gaagtaaaaa aagcagtggg ggaagatctt
     9601 atacctgtag gaaagatgct ggcagcagca gaaaaagtag tatacgtaaa ataataatag
     9661 caggcataaa accggattga tcatgaatgg aactcagtgc gaggtgtttg ggatattcaa
     9721 gagttgtgtg cattctttgc tgacagtata aataactatg aaatttacag acaatgaaac
     9781 tcttaataac actcttactt ttaacgcttg tcaagcagac tcactaatag aatttttatt
     9841 caatttacca gcagattttg acaacttaac agaagatgat cttaaataca aagaacttga
     9901 acaattaaag ctgccacaaa catgaactgt gtaaaagacc aaacgaattt tgattttttt
     9961 acaattttgc tgaacaatag ttcaacttat acagattaat cttttcttaa ttgctattgt
    10021 attcgttatc ggttaccggc tgcatccata ccgttacacc attttggtta ttcgtgatgg
    10081 caatctgaat aaatggctga tcatggctag caccatgcca atgcggaaca ttcggcgggc
    10141 atttcaccac atcacctttc cggagaagcc ttttcggact gcctttttcc tggtaataac
    10201 ctaagccacc ggttgccagt agaatttggc cacccggatg caaatgccag tttgttcttg
    10261 cacccggttc aaaggttaca tttcctacag cagtttgatt actgctatca ctcaggatta
    10321 actgttttaa ccaggcggtt ccactaaaat tattgttatc aattttttgc ccaataggaa
    10381 agacggcctc atcgggcatt gtaacgggag tatacggttt ttgattgatc acagcctgga
    10441 gtacctgtcc ggaaaccata cctctttttg gacttacctc tttttcaatg attgttacca
    10501 attccgccag ttgattttta gtaatgccat tatacatgcc tacgccaaag tgggagcgaa
    10561 gttgaggttc tgcaccctcc attgctgcca atgcagcaat cgtaattatt tcacgggtct
    10621 tccaatcgag attatctctc ccgaaaatag caccaaataa atgttctttc agaaactgat
    10681 caatggccgg tgcaaattcg taaacgcctc ccccgatttt tattcctata agttttgttt
    10741 gattttctgt tcctgcttgc agcatggttt tacgggcagg ataaggagat ggctctctgc
    10801 ctgctgcatc attgatactc tttttctttc tttcttccag caccgccatc aggttattca
    10861 gtgcattgag gctgcgggga aaacctgcat acgcatataa ttgcacgagc atttccttta
    10921 tttcactgat ggttagtccc tcattcaaac catcattcaa ggccacctgg agtttgggca
    10981 tattcccctg cgccgtatat gctgaaataa gaacaatgct gcgttccttt gaacttaata
    11041 aaatggtgct gtctgttttt gtttgcgcaa acatgccgga atatatcagc atacaaacac
    11101 agaggagtat tttatttttt ttcatttctg gctgaattag atgcttataa attttcacca
    11161 aagaaggagg tgagtttttt tgttgcctga tctacatggt ctggtttcca ataggtttga
    11221 atatgggtag ctccatcgat cagaaacaga tccttatttt tagcattgat tgccttggga
    11281 aatgcttcat ctgtcatata ctttgtatcg gccttgctcc cggccatcat taacaagggc
    11341 tgatcaatca gatccatatt ggtcgcagca tcaaaagtca ttaagtcaag caggctgctc
    11401 atcgtgtact taaaagttga attggggtgt gtatgggttc tgtaatagta cacatacccc
    11461 tcccgatata aatcggttgg gattttggca atttcctcat ctgtcggatt tgcgatcccc
    11521 gcataaatta cttcaccgcc tgcggcttct ttagctcttg catctgaagc ttgttttaaa
    11581 cgctgctgaa tggtggccaa ttgagagttc tgaaagccat tacgtcttac caaacccgaa
    11641 ttgaacatac tcagggtcgc cacggcaaca aatcgtttat ccccctgtgc tgcctttaat
    11701 gcataaccgc cgccgccaca gatacctaaa agacccaaac ggttcgcatc aactccggag
    11761 tactgcgtaa tataatctgc catgccgtga atgtcttcta tgcgattggc tggtttatca
    11821 acattgcgtg gactaccgcc acttgctccc tggtaagccg catcagccgc aatggtaata
    11881 taaccttgct ccgctaatcg ctgagcatac aaaccagcca cctgttcttt aataccgccg
    11941 ttgggatgcg ccactactac tgccggatat ttttttgtgg aatcataatt ggctggtgta
    12001 taaacattgg cggtaataac aatgccgttc agtttgtaac tcacgggatg aatattcacc
    12061 ttgcccggaa cattttcggt aagtgcagcc ccgtacacca aaccaaatgg attaggtgtt
    12121 tttgtttgag ctgaaatagt tgtaagtgtc aacataaaaa gaatcgaaaa aagatgaagt
    12181 ttgagatatg ttttcatgat gatgttctct ttatttattt tagaagcatt agtgaatcat
    12241 ttgctactgc aaaactatct cagaggtttt gcacagctgg tagacagatt gctgttttat
    12301 ctaccactat tactggtcca gttgtttttc tttcaaccag cctgacatca gatccgcaac
    12361 ttcaatatta tttaaatcgg aaaacggaaa gtgagtattt cctttgagac cggcttcagg
    12421 taaatgcact accgttacat cgcccccata tttgtttacg gcatctctcc actttcttgc
    12481 cattgcaagc cgtacccgcc aaccatccgg gccaggtgca ttggttggtt cttttgctat
    12541 gttgtcacca taataaataa caatagggat ttttgtaagc ttcataaagt cagacattgg
    12601 aacagcaact gcttctaatg tgcccccggc acttggcata gctgcaggaa cttctccttc
    12661 cggaaaaaga aaaccgcttc cgggttcata agaaacaatc gcttttatat tttgattttt
    12721 gatagcggtg cgccagccca taccacccga atgagagtga gtaaccagta taccagctcc
    12781 gattttatta aacagtgctg aaactgcttc agcatttaca ttgatgtcaa taggtccaac
    12841 attgggtacc atttgccgga aatactgatt cagcgtagta gagtcacggg caaactgaac
    12901 gccttcaaaa taatcaggcc atataccaat gcgaaatgtg ccaaaccatc cctgttcgtc
    12961 aggtgtggga gtaatgtttc cttcaactat acttcgggcc gcattccctc ttcttggctg
    13021 atcaaccagg taaacaggaa atcttcttcg taaaaaaata ttttgaaagc cttcacggcc
    13081 atcgggtgtg gtttcccatg ttttagaaaa ctggccgatg ccatgccaca taacaagcgg
    13141 gtattttctt gctttaaccg gaatttgata aaacacataa gcatgatcac cccgcaacgt
    13201 ttgcccggca gctgttggtt tgtaaggatc aaacacaccc ggatttgcaa ttacagtacc
    13261 tccggctgca aagctgccct gctcccggat agtgatgtac ttactgccaa cactattccg
    13321 gtttgttgaa catgacatta ctattaatga gacaatagcg gaaagcaata catgtgtttt
    13381 atttggcctt gtcataattc aaatttaatt tattatgctg cttatttctt tggaggcgct
    13441 tccacacctg acatattgag aactccttgc ggcagacgta atcctttgat ttcgatctta
    13501 gctaaatctg cattgaagct tatccattca tcagcagtaa accgtaaatg atcagcaccg
    13561 ttattttcta tcatgtgtgc catttgtgtt gtgcctggga tcggcactat ccacggcttt
    13621 tgcgccagca gccatgataa tgatatttgt gcaggcgttg cagatttttg aatagcccac
    13681 tttttaatca attctaccag tgccaggttg ttaggcaaat tctctggcga aaacctaccc
    13741 tcgccgccac gaatatcacc ttgtgcaaat cttgtattgg catcaatagc tccggtcaga
    13801 aagcctacgc ctaaagggct ccacggcaca aagccaatac cgagttcttc gcaaagtggt
    13861 ataacctctg tttccggccc ccgccataaa agtgaatatt cattttggat ggctgtaacc
    13921 gggtgaaccg catgtgcccg cctgatggtt tgtatgccgg gttctgaaag accgtaatgc
    13981 aatactttgc cttggcttat taaatcttta attgctccaa caacatcttc aatgggaacg
    14041 gcccggtcaa cccggtgttg atataacaag tcaatgcgat cagtacgtaa ccgtgtcagc
    14101 attccttcta ctacttcctt aagttgttca gggcggcttc taaagaattt tgtttctatg
    14161 acaattttgt ttcgaaaagg cgcaacagct tcgccaagaa tacgttcgct ttccaaagga
    14221 ccatatactt ctgctgtatc aaacagagta acaccattgt caaaagctgt tcgaataatg
    14281 ttaatcatct ctggccggga agggatagta gtttgataag tacgaggcat gttttgcaca
    14341 ccaagcccaa tgcttgaaac ttctaatgta ccgcccaact tgcgccgggt ggtcacctgc
    14401 tgtttagatg aattgctttt acctgcaact gaacctgccg acgttgtaca agcagaaggc
    14461 agcaatgctc caccagccag taccaaccct gttttaaaaa ggtcacgacg atttataccg
    14521 ttgcctgctt ttttgttgat tgaatttttc atgttttatg ggtttgaaaa ccggcatttt
    14581 tagtgatgct ggttatttgt tattactggt catttttacg ggtctcatca actttgagaa
    14641 ggttagtgaa cccatcgtcc attttccatt ctctttgata tatacttcag tcaccataaa
    14701 aggattgacc acttcatttc caccaaccac cgccacaagg tcaatgtcat ttaagaggat
    14761 gaccgtgttg ccaatagtgt tcacaattac agaatacact tcggcttttt tataccaaat
    14821 ggaaccgctt ttaataacat ctatttcccg ggctttcccc cagcttccac ccatatgaac
    14881 aaacatggat ttttcgtcga acagcacatt taatgaatca acatttctat ctgccatcca
    14941 ctgccacttt tgtttagaca ggctgatgat ctcctgctcc gcttttgtat accttttttt
    15001 agatatgctg tcctgattgg attgtgcgct gcaccattgt gcacacatta gtaagaaaca
    15061 aagcccggga atgaaacttt tcatacgtat aatttttaca agctgaagaa tgagattatt
    15121 tttttgcaga agctgcagta aaatgcaccc aatgcaactc tctttacaat gcaaagatca
    15181 gcgattatac cccctgcatg gtatacagat cacggattaa tctaccaata ttgctgatta
    15241 gtgtgtcgtg actaatgaat agatcaataa agcagtagtt ttgaccgggt aaaacaataa
    15301 ggatatgaat aacatgcgaa gatttgaaac catcaatgag tacaatgttt tcaataacaa
    15361 tgaaacacgg catccgttgg tcagtgttgt tgatttgtca aaagctgccc cgcggcaagg
    15421 ctcccggatg tattttggct tttataccat cttcttaaaa gatgtgaaat gtggtgacct
    15481 ggtgtacgga aggcatacct acgattacca ggaaggcaca ttggtgtttt tggcgccggg
    15541 acaggtggca ggcgtgaaca gcaatggaga aacctatcag cccaaaggct atgctttagt
    15601 gtttcatgct gatttcatac atggcacgtc cctgggacgt catattcagg agtatagttt
    15661 ttttggctat aaatcgaacg aggccttgca tttatctgaa cgggagagaa aaattgtact
    15721 ggattgtttt tcaaagattg aatacgaact ggaacatgct attgacaagc atagtaaaaa
    15781 acttattgca gataatattg aactgtttct caactattgc gttcgttttt acgaccgcca
    15841 gttcattacc cgtgataaac cacataaggg aattttagaa aggtttgaga ctttgctgaa
    15901 tgaatattat cagggagaca aacctcaaat atcgggattg ccctctgtgg cttattgtgc
    15961 cggtgagttg aatttatctt ctggttattt tggcgacctg attaaaaaag aaacaggtaa
    16021 aacggcacaa gagtatatac agtcaaaagt aattgacgta gcgaaagaac aaatatttga
    16081 tcacagcaaa tccattaatc aaatagctta tgatttaggt tttaaatatc cccagcattt
    16141 tacaaggctg tttaaacaaa gggtcggtgt ttcaccgaat gaatacagga accttaatta
    16201 acgatagttt tatccggaaa aaaaattctg ttcagcatac caacattatc tatagaaatt
    16261 aaaaggtatc atcgtccatt tatatgttat atcaaatctt ccttcactaa aaaaaaggac
    16321 aacattaaac tccgatactg cactatgccg gaagacagaa ttctattctc tgcacttcgg
    16381 tagtacctgt tcattatttt ctctgcaaaa aaatctctgc catcatacat cgtacgctac
    16441 ctccttccac ttgttcaatc gttggaaccg caattggtaa aagttcggca taagcttcca
    16501 gcattttctt ttgttccttt cttaaagaat caaatgcggt ctggcttaat attaaaatat
    16561 tttcgccttt gctatttttc acttccagca tgttgccggc aaaggaatgc atttgctctc
    16621 gggtaattgg aataattgta tgtcccgtgg attcgagcaa ttgtcttacc gctatcaatt
    16681 cccattcttc ctctatcgct tcttcacaga ggatacaaaa ccctgaaccc agggccatta
    16741 ccacattagt atgataaacg ggataaccat ttttatcagt agctaagaaa actattgcct
    16801 ggtagccatt ggtagcggca tatttttcca gtacagaaag gttggttctc tctgatactg
    16861 ctgcaaagat cattttatta tcatgatcca tcaccatact acctgtacct tccaggaacc
    16921 ggccttccgc ttcgtattca ctccagtcct gcacttcttt cactacaaat tcttttgcca
    16981 gttgcttcaa tatatcatct cttttttcat ttcgcctgtt gggggcatac attggaaaca
    17041 cggaaacgac accggccgaa gaagtgctga gccagttgtt cggaaaaatg gcatccggct
    17101 tggctggttc ttttgtatcg tcaatcacca ttacattgat agcatggttg cgaagctggt
    17161 gtaccatatt gtcaaactca gccagtgcat ttagctgcag cgtttcatta cttactcccg
    17221 tattttgctg aaaaaaatta tcgcctgctg tttctttatt gaaaccaaaa gcagccggac
    17281 gaatcattaa tatagtggaa gaagtgggca atgtgattgt tttagtaacc gctgtgtatt
    17341 atactggttt agataattca tgattttgta actgcaccag ttttacaatc ataactatca
    17401 gcgttctaat atggcgtcaa tttttttggc caacttatgg tctttatccg tcaccatatt
    17461 tccggcatca tgcgtactta accaaacttc cacggtgttc catacgttgg tccatagcgg
    17521 atgatgatcc attttctctg cctccagtgc cacccttgtc ataaaagcaa aagcttcgct
    17581 gaaatttttg aactggaatt ttttgtacag cttgttatct ttttcctccc acatgataat
    17641 gaatttacaa tgatcggatt tgagccggtc tttattaaac aaggtccctt tccggcagca
    17701 ccaattaaaa tacaaggttt tccttattct ttacctggtc aatggacagt tctgcgacta
    17761 actgaacgct gttaatactg cgaataattt gaatcgtttg ctgcattgtt tcatcattta
    17821 cctcatcccc tttcatcagc cataaaaaat caagatgttt gaattcgggc aataaatatt
    17881 ctccgtcaaa ctggttattg taaatataat gctccagtgt accagttggt actttgtatt
    17941 cataaacaga gaaaaagtaa tcccttttct ttttgatcag cttgatctca atttcattga
    18001 tacgaaagtc gaggccaata ttattattca ggtgccagca gaattgataa tctttaatag
    18061 gcgccatgat gcccagcagc cggatatcct caaagaaccc ttcagttaat tctttattat
    18121 ccaggaacag tttattgctg cgttcagcca taatttagtt tcctctgtaa gttacaattt
    18181 agaactcaac agttgtttcc agggtatcat tacctctttt gtaaatcact tttgttttat
    18241 cccccttttt aaacttgccg agtgtctgca tataagtttc catagaggtg atcttgtagt
    18301 ctcctatctg caaaatgata tcacctgctt ttagtccggc cttttgtgcc ggcttacctt
    18361 cgcttacccc atcagctctc aggccggtac cggagtaagt ataatcaggc atcactccca
    18421 ttgttacact aaagcgggca gaagtggtgg tttgggtttc ccttgttttt aaaaaagaaa
    18481 gcttgctgct gctgttcagc gattcaacca aaccggtagt atgttgtaag atggatgcaa
    18541 taccattata atttatttta tctgcatcat cagacggttt gtgataatcc gtgtgcaatc
    18601 ctgtgaaata aaaaagtacc gggatgtctt tccggtaaaa agatgtgtga tcactgggac
    18661 ctgtgccact ggagtcaatt tttacagtaa tgctgttacc aggagccata gagatcactg
    18721 aagaccaggt tggtgaagtg ccgtagcctc cgatagtaac ggatttagta ctgtcgctga
    18781 gtctgcccac catatccata ttgatcatgt agttcgccgt cttcaaatcg atagtagggt
    18841 tttcagtaaa atattttgat ccgtataaac ccaattcttc accagagaaa gcaatgaaga
    18901 gataattatt gttgttagct tttgactgtt tcattttttt tgccagctct atcagggctg
    18961 ctgttccgct ggcattgtca tcagcaccat tatgaatgat gcgttcagtg ctggtatttc
    19021 ttgaattacc atcttcccca taaccaaggt ggtcaaaatg cgccccgagc acaacagtag
    19081 tggcagctcc gttatcaata taaccaacta cgttgtggcc tgtccttttc ttttcactta
    19141 gcgctgtagt aaattttata tcaatcgtgg cagatggatc tttaaaatat ttctgtgcct
    19201 ctttcctggt tacgtataag acgggaattg tcaattgttc tgacttgtct ttaccattaa
    19261 acgtcagttt atcatctata gatgaggtat tgtacaatat aacagctgaa gcgcctctat
    19321 ctttcgcctt tttagaattt gtataaatat aattagccag gtcaaagtgg ggattggctt
    19381 tgttttcctc taactgctct ttcagatcaa aaaaccaggg catatctgct tcctgtacag
    19441 aaatggaagg aagcgcttcc attttttggt tgagactgaa agggaaaggg aagaaatcct
    19501 tacccgcaac caggttgttt ccgttgatga taagctttgt tgaatcgcca atttgctttc
    19561 cttcgttaat ttcaaatggc tgataataac ccttactgcc tttgggcaac aaccctattg
    19621 acttgaactg gttactgata tattccatcg ccattttttc cccattggtt ccggttcgac
    19681 gaccctccaa tttatcatct gccagatatt ggatatgcaa ttttaaattt tccagcgttt
    19741 gtacatcagc tttagtgact tttttggctg gtgaacaggc tgcaaacacc aaaacaacca
    19801 caacaattat tcttgaaaaa gaggaaatca tacaaatttg attgatgagt tgcaaaggta
    19861 ttgagctgct ttctttttct ttgggtttat catcatattt ccgtcattct tttccggatt
    19921 gctgcgccta aaaatcgtaa ttcgtaaggc ggtttcagct tcccgctcta cttttgcctg
    19981 cttgtaagtg ttattataaa tacgaagtga aggaaaaaag gctacatatt gctctggttg
    20041 gtaaccctaa cagcgggaaa acctccttgt ttaactgtct caccgggcta aaccagcagg
    20101 taggaaattt tccgggggta acagtagaca aaaagactgg tacaacttcg ttgggatcaa
    20161 tccttgcaga ggttattgat cttccgggaa gctacagtct ttaccccaaa aggctggacg
    20221 agtgggtaag ctataaagtc ctcataaacc aggataaaga catcaatgcc gatgtggtgg
    20281 tggcggtggc cgatgcaagc aaccttaaac gaaacctgct tttttgtaca cagatcattg
    20341 atttgaaggt tcctgtggta attgcgctaa ccatgatgga tttggcaaaa cggaaaggaa
    20401 tcaaaataga tattgctgcc ctggaaagag aattaggcgt tcctgttgtt gcagttaacc
    20461 cccgcaagaa caaaggaatc gttgagttaa aaaaagcaat cggtcttact gcgcagcagg
    20521 tttataaagt acctgcccgg gattttattg acaataagtc cctggcagaa acttccattg
    20581 aaaacattaa gcagcatttt cccgaattaa gcgattacaa ggccattcat tatctcatta
    20641 atcatgaatc ctttgatttt aatgcttccc tgcaggaaac aattgagaat attgagataa
    20701 aaaataaatt taaccctacc aaaacgcagg cagaagaaat tttacagcga tatagccgaa
    20761 tccggcaggt tatgcaacag gcagtatctg aacctgatcc gcttcaaaaa acactgttca
    20821 cagaaaaact ggataatgtt cttttacatc gtcgctgggg ctacctgatc ttactggcag
    20881 tattgttttt gttgtttcaa agtgtttttt ggctggcaca gcacccgatg gactggatcg
    20941 aactgggttt tgccaaactg agtgaatctc tctcctcttc attacccgat agtcgctgga
    21001 ctgatttatt aataaatggg gtgattgcag gtctcagtgg cattctcatg tttgtaccac
    21061 aaataatgat attatttggc ctgatcactc tcctggaaga ctccgggtat atggcccgta
    21121 tcagtttcct cagtgacagg ttgatgcgaa gtgtgggact caatgggaaa agcgtaatgc
    21181 cgatgatcag cggatttgcc tgtgctgttc ccgccatcat gagtgcaaga aatattgaga
    21241 accggaagga acgtttactg acaatactga taactccatt gatgagctgt tcggcaagac
    21301 taccggttta tacgatattg atagggttag taattccaca aacaaacctg ttaggatttt
    21361 taagtgtaca gggtttggta atgatggggt tgtatttact gggtttagtt atggcattaa
    21421 tagtttcgta tgtagctaaa tggtttatca atatcaggga aaaaagtttt tttattctag
    21481 aattgccaac ctacagatca cccaggtgga ataatgtgct gcctacgatg atcaacaagg
    21541 cccgcatatt tgtaatggat gcgggtaaga tcattatgat catcagcctg gttctttggg
    21601 ggctgagttc ctttgggccg ggtaatacga tgcagaatct tacacaacag tatgatcaga
    21661 tgaagacaca acccggagca gccattgcgc aactagacaa agaatatcac acagctaaac
    21721 tggaaaattc gtatgccgga attatcggca aatcactgga gccggctatc gctccactgg
    21781 gttatgactg gaaaattggt attgcattag taacgtcttt cgctgcaaga gaggtctttg
    21841 tcggcaccat ggcaacatta tacagcgttg gagacactga tgagggcagc ctgctgttga
    21901 aggagaaaat gaaagcagca gtaaaacctg acggaacccc tgtctttaca ctggcaaccg
    21961 gtttatcatt gatgatcttt tatgtgtttg ccatgcaatg tatgagtacg ctggctgtgg
    22021 taaaaagaga aaccaggagc tggaaatggc cggttataca attgatctat atgacagctc
    22081 tggcctattt gatgagctgg gtggtatatc aaatatttaa gtaatcctat ttcagcccag
    22141 gttcccattt atcctttggc tcaaaatatt cccataatat cctggagatt tttctcgtca
    22201 acacccaggc ttcattggtt tcaccccagc ttatgtcttt attgtttttg gtgaaaatgg
    22261 aaaagatata gggattatga atggcgttta ccaataatac ttcacttctg gatgcattga
    22321 ctgcaccatt tttgctgaac acctctatgt atggtggaat ttgagacaag gcttctgcgt
    22381 cccagaaatt acggccgaga caacgcatca ttctttcaca ggcagctggc gaaattactt
    22441 tgtttctata gatcatttca aacagcatcg ccatttcgga tggactggtt tgtccccacc
    22501 cgtattgtgc ccggttaact tctcttccgg gtgtcctgga gttaactctt gtatatttca
    22561 gtcccaggct gtccattatt tcattgatcc ccgttccggt tccggctaaa ctttgcaacc
    22621 aaagactggc cgtattatca ctggtggtca gcatcagcat gatgactttc ttcaacgcaa
    22681 ttttttcatc tgttttaaaa gatcccagta tatcagaccc tgcatataat aaggaatctt
    22741 tgtataccag tgaggaatcg tagtgtagtt cacttctttg tattttatcc attattccca
    22801 gcagtatcgg cactttaaca atgctggcgg taggaaaaac agaatctgcg ttaattgcaa
    22861 ctacttttcc tgtccgcaga ttttttacat aaatacctat atcgccttta aagcccctta
    22921 taacctcttc aagctttgcc ttgagttttt tatctgtttg ctgagcaaac aatgtatctc
    22981 tcccggcaaa aaaaacaaac agaattaata aaacagattt tttgttcata atcagaatcc
    23041 ttttttcgtt tgagcttttg acatgttcat ataatccaaa accatgtggc aaaaggcttt
    23101 tataccggtt ttcataccac tttcatcaat ataaaaatcc ggtgtatgat gtggaaatgc
    23161 ttttttagca tcctttcctt tttccatgcc accgataaaa aagaacaatg aaggaacttt
    23221 ttctgcaaag aaagaaaagt cttctgcccc ggttatagct tctgtttcaa ctacattatc
    23281 cgcacctgca gcttttataa aactggggat cattttttta gttagtgccg gttcgttata
    23341 cgtcaccaat gtttttttat caatcactgt ttctgcggtg gcaccggtac tttcagcaat
    23401 tttggaagcc gtcaaaatta ttttctcatg gataattctc tgcatcccgg tatctaacgt
    23461 acgtattgtt cccagcatat tcacttcttc cggaatgata ttggaacgaa cgccaccatt
    23521 gatcacactt acagaaatga cagctggatt ttttgttagt tcggtttgcc tgcttacaat
    23581 tgtttgaagt ccgttaataa tctgtgcaga taccactacg gggtcaattc cggcccaggg
    23641 ttgagagcca tgggtttgtt ttccttttac ttttatctta aaccaatcag aggccgccat
    23701 gatgccacca accctgtatt ttattttacc tgcttctgtt tgtgaattga tatgcaatcc
    23761 aaacatcaca tccacttttg gattttcaag tactccgtcc ttaatcatga gtggcgcccc
    23821 gccttcttca ccttcgggag gcccctcttc tgctggttga aaaataaact tgatcgtgcc
    23881 ggttaattca tttttcatac ccgccaatat ttccgccaca ctcattaaaa tagccacatg
    23941 ggcatcgtgg ccgcaggcat gcattactcc tacactttgt ccgttatagg ttgattgagc
    24001 tttggaagca aaaggaagat ttactctttc cacaacaggc aatgcatcca tatctgcccg
    24061 caagccgata caaggaccgg gcttagcccc tttcaatata ccaaccaccc cggtctttcc
    24121 aatattttct tttacttcaa tacccagaga acgaagatga tcagctataa tttttgcagt
    24181 acgaaactcg ctgttaccca attcagggtg ttgatgaaag tctcttcgcc agttattaca
    24241 cttagcttct attttatctg tagcgatact gatcttttca gctatatccg gaccattttt
    24301 ctgtgcaaaa agaattaccg gtatcagaaa tagcaaaaga cttagttttt gtttcataaa
    24361 cgaaatttgg ttgaatttac attaaatcat ggctgattta atttctgatt acccgcttag
    24421 ttgatttttt ttatatttaa gttccaatgc tgctgcgtca tgcttcattt ctccgggctg
    24481 tttttataca cagcgttacc gcttttggcg gaccccaggc acatatcggt atgatgatga
    24541 aaacatttgt acgcaataaa ccatatgttt ctgaaaagga gttaatggaa tacaatgctt
    24601 tttgccaatt attaccggga gcttcttcca cacaaacact tacgttaata ggatataaaa
    24661 gaggcggagt gccgctggct gtattaacat tactcatctg gattttaccg gcttgtatcc
    24721 tgatgggagc tttttcattt ctattacaat acattgataa aaaagaatta ggtacagata
    24781 tttttaagtt tattccgcca atggccgcag gatttttagg atatgcggtc attcatatat
    24841 ttccacatgc aatccgaaat accattacct ggataattat gctatgcagc gtggctgcta
    24901 cttatttctt atttgggcga acctgggtta ttcctgcatt aataatagca ggaggtattg
    24961 ctaccaattt tagtctgaaa cggataccgc aggtagagca aaaaccaaga aaaataagat
    25021 ggtggaattt ctggttgttt ggtattattt ttctgctagc cggaataacc agtgaactgg
    25081 caagaaaaaa cgactggccc aatagaaaac ctatcaacct gtttgagaat acgtaccgga
    25141 tgggaagtct tgtatttggt ggagggcagg tgctgatgcc aatgatgtat gaacaatgga
    25201 gcataaggcc cgaagctgta cgaagcaaaa atcccaatgc agtacaaatt gatcaggaca
    25261 aattatatac tggtatgggt atagtgcagg cagtgccggg cccggttttc tctattgctt
    25321 cgtttgccgg cggcatggcg ctgaaagata tgggaacaga aatgcaggtg cttgggtgta
    25381 ttattgggat gataggaatt tttttaccca gtgcattatt ggtactgttc ttttttccta
    25441 tctggaataa ccttaaaaag tatgccgcgg tatatcgttc gatagaaggc attaacgccg
    25501 tagtagtagg cgtaatgatc gcctcttcct tttatattat gagagatatt tctttcatag
    25561 aaccgaaaac agtaagcctt gtaaatatat tggtgatcct gggaacgata atactgctaa
    25621 attttacccg catactacct ccattcattg ttattacctg tttgttgctg ggttatttct
    25681 tataatatcc tccccgctct atttcttcca ataccttgtc aggtactaaa taccgcagcg
    25741 atttcccttc ttttgcatac ttacgtattt gtgtagccga aagctgaagc aacggggcgt
    25801 caacgaaatg aacatcggcg ttgagcttat cttcaatttc aaacccggga cgcaggtaaa
    25861 cataaatagg atagtttttt acaatcactc cataattttt ccatttggga agattctgaa
    25921 aactatcgct tcccatgatg atactgaatt catgttcggg atgtttttct gacaggtagg
    25981 cgagggtgtt gactgtataa gaaggcttgg gcaacgtaaa ttcaatatct gacgctctta
    26041 tccggttgtc attttctgtt gccagtttca ccaggtgcag ccggtgatat tcgttcaata
    26101 aagaattttc ggttttgaaa gggttttgag gagaaacgac aaaccatacc ttttcaatat
    26161 ctgtttcgtt cagaatatga tttgcaatga tcagatgggc aacatgaact ggattaaaag
    26221 aaccgaaata aagccctatt ttcatttata aatgattgat aatcaattat ttatttcgac
    26281 caatatatgt tccgcctcat tcagcctgag gcttaaatga tacccggcta catttaccga
    26341 aataggatcg ccgaggggcg ctatctgttc caacaggatt ctttcaccag gtacacagcc
    26401 catttccatg agcttcagaa atatatcgtt gtcgatgaat tcccggatta ttccttgttg
    26461 tcctggtttt aactctgata gccttgcaat catttaccca atattattat acaaaactac
    26521 ccttgttggt tggtttttct ttacgaattt tgaaattatg ccctcaaaat caaaagtttg
    26581 tttcttttat gaatataaaa aatttagcct ggagaaccgg ggtgcactaa agtttttcat
    26641 tgaatcaatc tttaagaagg agaagagacc cctttcctcc atcaattata ttttttgctc
    26701 agataaaagg cttttggaga ttaaccagca attcctcaag cacgactatt atacagatat
    26761 tattactttc gacctttctg ataccaattc tattcatgct gaggtatata ttagtattga
    26821 acgggtaaaa gagaatgcta aatcattagg ctcctctttt aaagcagaat tacaccgtgt
    26881 aatcttccat ggagcacttc acctctgtgg gtttagggac aaaaccaaaa cagagaaaga
    26941 gcatatgaga caaaaagaag atgtttatct ttcccgctac ttcaaataat gtttcacggg
    27001 aaacattttc ccttttttcc acgtggaaca ttttccctta ttagtattcg cagatcctaa
    27061 attgtacaaa gacaagataa tcccgtttat gcttgtacct ttgcaaactt atgttttcac
    27121 agtatgatgt aatagtggta ggtgccgggc atgctggttg tgaagcagct gccgcagctg
    27181 caaatcttgg ttcccgtact ttattggtta ctatgaacat gcagactatt gcccaaatga
    27241 gctgtaatcc ggcaatgggt ggtattgcaa agggccagat tgtacgggaa attgacgcaa
    27301 tgggtggtta ttccggaatc gtaacagacc tttccactat tcaatttcga atgctgaacc
    27361 gcagtaaggg tcctgccatg tggagccccc gtgctcaaaa cgatcggatg ctttttcatc
    27421 tgaaatggag ggaaaagctc gaacaaacaa tgaatcttga tttttaccag gatatggtaa
    27481 aaggactatt ggtaaaagac ggcaaatgct atggtgtagt gaccggaatg ggacatgaga
    27541 taaaagcaaa atcagtagtg ctgactaacg gtaccttttt aaatgggatt gttcatatag
    27601 gcgaaaagaa ctttggtggt ggaagaatgg ctgagaaagc tgctaccgga attactgaac
    27661 aattagtatc ccttgggttt gaaagcgacc gtcttaaaac cggaacgccc ccacggattg
    27721 atgggcggag cctggattat tccaagatgg aagaacagcc aggtgacgag gaaatagttg
    27781 gcttttcttt tttagaaacc gaaaaaccaa aacaacaaag gagttgttgg ataacttata
    27841 ccaatcaaac agtacacgat attctgaaaa ctggtttcga cagaagcccg atgtttgccg
    27901 gaaggatcga tggggtaggg ccaagatatt gcccaagtat tgaagataag atcaaccgtt
    27961 ttgccgaaag agacaggcac cagctcttca ttgaaccgga aggatggaat actgtggaaa
    28021 tatatgtaaa cggattttca acttcattac cagaagaaac acaatacgaa gctcttcgta
    28081 atatagtggg ttttgaaaaa gtcaaaattt tcagacccgg gtatgctatc gaatatgatt
    28141 tctttccccc tacacagttg aggtattcgt tagaaacaaa gctgattgat aatttattct
    28201 ttgccggaca gataaacggg acaactggat atgaagaagc tgcctgccag ggacttatgg
    28261 caggtatcaa tgcacaccag aaatctcatc atcaagagcc gtttatacta aaaaggagtg
    28321 aagcttatat tggtgtattg attgatgatc ttatcagcaa agggacaaaa gagccctatc
    28381 gaatgtttac ttcaagagct gaattcagaa cattgttgcg tcaggacaat gctgatctgc
    28441 gtttgaccga attaagctat cgtcttggcc ttgcttccca gcaaagaatg gataaagtaa
    28501 ttgaaaaaaa ggcatccatt gagaaagtaa aaaccgttct aaaagattta gccgtagaac
    28561 caaatgaaat caacacctgg tttcaaaaaa tcggatcttc ccccatttct gaaaaacaaa
    28621 aagcccaaaa gatactgttg cgtccagatg tagaattaga tgagtttgca aaagcctttc
    28681 caaaattaga actcgcttta caaggccttt ctaaagaatc ggttgaacaa gcctctatac
    28741 aggttaagta tgatgtatat attgaaaagg aaaaggaact ggtacagcgt atgagccaat
    28801 tagaagagct ggaaattccc gaaacatttg actataaaaa aatctcttcc ctgggtaatg
    28861 aagccagaga aaagctcact aaaataaaac cccggacgct tggacaagca agccgcatct
    28921 ctggcatcaa ccccagcgat gtccagatat tgatggtgtt tatggggaga tagtttttta
    28981 tatccaatgc ttttttaatt tccctgcgct ttgctcatct gtttattata gatcaattat
    29041 tacctttatt acaaagcgaa tagtatgaaa aaaaatgttc tctgcttcat tgcagtgatg
    29101 ataactgctt tcgtaataga taatgaagct acggcccagc aatataaatt gcgtcagtca
    29161 accagtatga tgggaatgaa aacagagtcc acaatttatg ttaagggaat gcgcaaaagg
    29221 accgaaagtg ccggcatgat gggaatgcca gccccaccta taaccattga acaatgtgat
    29281 ctgcaacgta ccatcaaaat aaatgacaaa aagaagctct actatattga atccttctcc
    29341 aaggaaaacg aagaggtggt tgatgaagat gcaaaaccgg ctgcccctgt aaaaaataaa
    29401 cctgtaactc cggcacctaa agaaaaaaca accccgcaga agggaggagt tatcaccatg
    29461 tggtacaata ttactgacac cggtgaacgc aaaaatatct acggctttaa cgccaggcat
    29521 gtgtggactt cccaaaaaat taaaccatcg ccagatgcct gtactatgaa agatagtatc
    29581 cttattaaaa cagatggctg gtacattgat cttcctttgt ttaattgccc ggttcgctat
    29641 cgccccgtta aagcttccat ggctccaaca gacaggcccc aaccagattg taaagatcgc
    29701 tttgttaccc ggcggagtgg aaaaggaaaa ttaggtttcc ctttaactga aacaacaaca
    29761 attaaaatgg gcggtgggaa tgcacagatg agtgaatttc aaacttctat tgaaacgctg
    29821 gagctatcaa cagtaaaact ggattcaatg ctatttgaaa ttccaccggg atatacagaa
    29881 acactgaacg aggatgatct gcaggaaaaa tttgatgcag gtgatatgat gaaccaggca
    29941 atgaaagaat acaagaataa agcagagaca aagcctgccc ttactgaaca aaaaaaagat
    30001 ggtataattc gcattggtgt gtatgagccc aggggggatg aacagttgca ggcttcggta
    30061 ttacaacaac accttgtcaa tactttgaca gacggcactg ttgaagctat ttcaattata
    30121 tcagaagatg atgcaaaaaa atataattgt gattatacac tggccagtga ttttattaaa
    30181 ataaaacaag gcagtaaagt gggcggtttg ctgaaagcaa ttaaaaatac agacccgaac
    30241 gccgcatcat cgttcacaat tgaagcggct atgacacttg taaacttatc tgatggttct
    30301 acccgtctgc aacccaaaat aaatgggaaa tatgatggaa aagcagatga agctgccaaa
    30361 aaagcactcg acgatggctc ccgccaggta ctaagagctt taaaataaat taatggatta
    30421 aatgaaggcg acagatatct gtcgccttca tttaatcagc tctcaggtta ccctaatata
    30481 gaaaagctat tagtaataaa ttatgctaca accttcactt ttaaattaac cggatctggc
    30541 attctcaaat cttcttcctg cagttcaatt cgtcttgtaa tgtcatttaa agaggacagt
    30601 cgttgtactg tataaggaat ggtatcgagc ccggaaacaa atcgctggat ataactataa
    30661 ataccaatta ggctacccgc ctctattcca gccccggttg ttttgttcgt aattaataat
    30721 gctaaaccaa ttaccaccat taccattatt tccataatac cgaaattcca ggcctcctga
    30781 tcgctgatac ggatctgcca ttttcgtaaa ttatcataat gctgccgaat caaatggtta
    30841 ttgccggtgg aaataatatc tacctggttt tcgagttcat cattcttctg cttattcagg
    30901 ttacgcatct ttttcccata gaaataactt aaaccggtta caggtaataa aatacccagg
    30961 caaaccataa caactgaagt atcatagaaa aacaacaata acagtgaccc tacaatacta
    31021 taaaaagcct ctacaacata tgccagatca aactcaagaa aatctacaaa ctctctcgct
    31081 aatgtggagt gggcactaag ctttgaaata tccgatttat tatagcggcg ggccagaaat
    31141 tttgtaacca atgaagtata aattgctgag taggttctgg tatcaagccg gtgtcttata
    31201 gttccgataa tcaaccaccc taaatgaaca gcggataaca aaacgagtcc tttgtagctg
    31261 ccctttatca aatcatccac cgccagacca aggaaaaagg gacggagcag gtttcccaac
    31321 atttccagtg aaaaaagtac ataggtgaac agtaaccggt agcggtgctg taacataatt
    31381 tgtttaagta tggcaaaacg ggacatagtt catcttttta cgataaatca ccaggtttat
    31441 agtttgtgta ttcacaaacc gccgaataaa tttaatggag taagcctgag accagcagac
    31501 aggttagtcg tttaaccgga gtaaattttg ttcccgcaaa tttagcgaat tgccggtgtg
    31561 gccagaaatt tttccccggc aggagatgga accgcactga caatcaaagg gttccatatt
    31621 ctcatctaaa aaatcagcgt agtcaagagt gagttcatgt cctttagata tattcttaat
    31681 agctaataca ttcaggccat caaaagacgt attcggatcg caacaatggt tttgtggcgc
    31741 ccattcagat ggatcatcat cccagagtat aaagacttca tcgctgatag ggtaagcgta
    31801 ccggcgaaaa tgtagttttt cgtcttcatt ccaattcctt tccacaaatc gttttgtaat
    31861 aattcgctgg gcccgaccct cccctttgaa gatcacttca cccgccttaa tatcacggcg
    31921 ggcataaata ccataaccgg caatggaatt tcccttcatt acgaaaggtt tcattttacg
    31981 tttatgccgg gaaatacctt cggcaatcat atgccttaag aatcccgcct gcccgatccc
    32041 atcatacttc agaataaaat cagcggaacc ttcataacca tctttataaa aaacagaaca
    32101 ggtaaaatta atttccagaa agtatatctc attcttatca ttgacccgaa aatctaacct
    32161 tgcataccca acgccattga atcctttaaa aattttttct gtggcgttac gaagctgttt
    32221 atccaatagt tcatcattac aaggcacatt acaatcagga tgcagttccg atgtttttaa
    32281 cgcataggtt ttaaattccc ggccttcggg aaataagtat tcaacgggcc gatagacagt
    32341 acaactgtgt tcatcggtat tagcagctac cagcacggtg aattcccttc cggcaacata
    32401 ttcttctacc agcagagggc cgtactcgtc cagtatgctg atacattttt gttgcaattc
    32461 aattttgttg tgcaccagcg actgttcgtc aactccaagg ctatcacctg ctttagcagg
    32521 tttcacaaat aaagggaata acaaatgttt aaccgcatca tccacgtcat ttaattcatt
    32581 aataatgaca taccccggtg ttttcactcc ttcgcaatag gcgaggtatt tcattaattc
    32641 ttttggcgga tcataaagct tagttgtcgg tccggtaaaa ggaaggttca aaatttccag
    32701 tgtataaata acatcaatgc tgggcacttc ccattccaga tagccttcgc aaaggtttac
    32761 aaaaatatca tagccatctt tactgagttg cttcagctgt ttataggtag taagcttatt
    32821 gagaaaaata gtatggactt ctgcttcggg aatcaatacg gataagtccc ttggggggtc
    32881 gtaattttta taatcaactc cggtggtgga gtagtccggt tgcagtacac aaactttcat
    32941 gaattgctaa ggtaataagt aggaggatat aaaaacaaac ttcgttaaat ccgataagta
    33001 ccggtaacta tttgaccaca atggtttttt ttattttggc tactttggct ttctttaccg
    33061 gaacctgctt tacgacgctc cttcgcaatg catctttgag tatttcggat ataacttcag
    33121 tgaatgatac gttggaagcc ttcagaatgg ctccaatgga agtatagttt tcatcttcac
    33181 ttaatccgca ctgcgcattt gcttccagca tataaagctt accggttgaa gaatcctggc
    33241 ggatgtcaat tcttgtgtac cctttacctc cacaggcttt gtatgcatca aggctgagtg
    33301 tttttaatgc agcaaccagg tcaggcttta cccgctggta ctcataaaaa ttttcctccc
    33361 gcggcattgg tgtttcatcc tcataaattt cccagaggcg gtcaaatgat aaaaatttct
    33421 ctgaatccgg taaagaagta tgaaaaaccc tttctacggg ttcgtatact ttacagtttt
    33481 ccggatcatc agcagagccg gttataaaag tggtgaactc ggggcctgta ataaattgtt
    33541 caacaaataa tccatctgcc agtaaattcc agccccggta tcctttttta atttcttcta
    33601 ctctttcagt cagctcttct tccgtaaata ctacattctt aacagagact cccatactgc
    33661 cacccgaaac agctggtttt ataataatag gtgtgccaac cctttttgca attccttttg
    33721 tcgatccctt tttatctgta atcacccgcc agttggctga agaaaccccg gctttgtcaa
    33781 aagccctctt catggggatc ttagaagtgg tgatagcata gaagtgcgca tcagaaccgg
    33841 tgtatatcaa tccatatttc tccaactcat gaataacgga aacacctggg gtaccattta
    33901 cctcatcgcc atcacagaga ttcagaataa gcggagtttt tccatttttg ctttcagcta
    33961 tagatttgat gacaatctta aaattttcca ccgttaccgg ctgccatttc cactctgcct
    34021 ttagctcttc aaatacttta gtgtattccg caatactttg ggtaaaatcg tagtagtatt
    34081 taagattagg atcttccgat tcaaggtggg gggccagaac ccagataaaa tatggcttta
    34141 ataaacggga ggcttctgct gtcttcatca cggatcaggc tacttttctt gccgcctttt
    34201 tggccagttg ttgttgaata aaatcagtaa agcgatattg tgcagcaata tcttttgata
    34261 aaaaagcagc ccctcttatt tcaccgatac aggcaggact accacaataa caactgaaag
    34321 attgtgtcat ttcccattcg gtagagggat aaaaaaatgt catttcttct tctacatcca
    34381 attcccttaa tgctactaat tgcattgcag aggtatcaaa aaatacattc gggctacagc
    34441 tatgattgat gtactggaga aactccggct gcagtgtaat atgtttacca acaccaacct
    34501 gtacagtcag gtacgttggc tctgcagcaa tagtgccggc cgaaaaatca gcaattacct
    34561 ctcccggctg atagggcctg agagcaaata atgcattctg atcattggaa attttttgac
    34621 gcacttcagc aattacatgg ttacttataa tgcgataagc agggtttgta agtgtctcca
    34681 taaatgtttt agtaagtatt cgaatagaaa gatatatata ttgtttaacg taacaaattc
    34741 ataacagtca aatgtgaata attaataaac aatgtgaact atgaaagaca attgtctgtt
    34801 gagtttattg atcaatgaaa tcatttatga tccgttcccc gttaccgtat aaattaaaac
    34861 aattctttca gtgtacattt gttgtttgct ttgataacaa gaaatggccc cgtgcccgaa
    34921 tgcaaacaat gatgacaagg ttagtttatt tatgctgtta ctttctgctg ggatatgctg
    34981 atagtaaggc ccagtgcagc tctgccatca acagctttcc ctattccgaa agctttgaaa
    35041 caactgatgg aagctggatt tcgggaggtg ttggcaatga ttgggcctgg ggagtgcctt
    35101 ccaaaccggt aatatcagca gcaggtggtg gagtaaaatg ctgggtggtt ggtggattaa
    35161 ccggaagctc atataccaat tcggaagcat cctggttgca aagtccttgt tttgatttta
    35221 ccaatctgat atcaccttat attgaattca agattttttg ggagtctgaa caacgatttg
    35281 atggagcaag ttttcaatat tctacggaca atggaattac ctggcaaacg atgggctctt
    35341 ctgccaatac agttaattgt tataatgaaa actggtataa taacagttct attacttacc
    35401 tgtcaccatt agccagtaat aaaatgggct ggtccgggaa tattcaaacg agtagcggat
    35461 cctgtgccgg aggtgccgga agtggaaaat ggttaactgc aaaacaagcc atgggttttt
    35521 tggaagggaa acccaatgtc atattccgtt tcatttttgg tgctggttct atttgcaata
    35581 attatgatgg ctttgccatt gatgatatac ttataaagca agcccctgct tattatgctt
    35641 cttttaccta tacctgcgat ggagaaggaa ttgtttcttt taccaacact tccgatgctt
    35701 gtgtaacaat ccagaactgg gattttggtg atcctgcatc aggcgcaagc aattcatcgg
    35761 atattccaag cccaacacat aaatattcgg ctccgggaaa atatacagtt aaactgcatg
    35821 ccagttttcc gggtagctca atcagcacag caacacaaac aattacaatt cctgatgtca
    35881 gggtcaccca attaagcccg gcagactgcc aaacaaattc aggcggttca ataattgcaa
    35941 acgtaagcgg tgtaaataac ccggtgaatt attcatggaa tactgttccg ccacaaatca
    36001 cacaaatagc aagcaattta tcagcaggtt tatacactgt taccataacg ggtacagatg
    36061 tttgcccgac taaagcaact ggagaacttg tgaccgattt ttcctgcccg ggtatatttt
    36121 ttccttctgg ctttactccc aatggcgatg gtaagaatga tagctttgga ccgataggcg
    36181 gattgggtgt aatcagtaat tatcagttca gtatttataa tcgctggggt gaacgggtct
    36241 tttattccac caacccattt gaaaaatgga atggtttgtc aaaaggtata aaagctgatg
    36301 gcaattcgtt tgcctggtat gccgaattta ccttgcccgg caaaccaaag gaatcacgaa
    36361 aaggaatagt tacattaatc cggtgatttt tccgggctaa tacctagtcc ggccataacc
    36421 aatcttattt ctccaaaact tatatcgtca ccaagccttt tctttaaggg ggttattgag
    36481 ctgccactaa actcttccag ggctgattca atcaacacca gcttttcccg gcttactaat
    36541 tcgctaatct ttatatctcc ccgtcgcaca aaagtggtta agtggccttc aatggtagaa
    36601 acagccagtt ttcttgtttt cgcaatagca gctattgact gcccttcctt gtacagcctg
    36661 aatgtttcga tatgagtatc cccctttttt ttgcccggac tgattagttc cttttttctc
    36721 tttccggata ctttttcgtg tattaaggac tgtaagttgt gctccctggt gtattggaga
    36781 atgatatcta aaaactgttg cccgtattgt tcaatcttag tatctccaaa gccactgatc
    36841 tttcttagtt ccgttagtgt ttgtggtaaa tacatcgcca tttcatcaat agtattgcta
    36901 ccagctacaa tataaatagg taaatccctt tttgcacaaa tactttctct gagttttcgt
    36961 aactgctgat gcaaaaccgg atgagggctg tcaaaccttt tttgcgaagc gccggcatat
    37021 gcattgatac tgaaagaggg caaaacaaat tgtagtttcc tccggtgaaa tgcttccata
    37081 tctaactgac cattataccc ttcaagcagg aacttcttca tggcgagcag ggcaaaaact
    37141 tcccggatac tttcattata ttcctttgca ttaagccggc tatcggtaat tactggtgat
    37201 gtgtgaataa attgaagtaa acggttgatt tcattattaa aatatactgc ggcaactttg
    37261 atcctgtctt gtaaggctat gttctcttcc ggttccaccg gttgctgaaa taaggcccgg
    37321 agttgcgttt ggaatttctt tgcagtcact tgtaagccac ccagcttttc cgaaagctct
    37381 tcagtccagc cgaaggaatc cgggttaaac gaagaagtgt gttccagtaa ataatccatt
    37441 atttctttgc agccatcgat ggcattactg aaatcaaata ctcccgccaa aacagattgc
    37501 tgataattct ttttagcaat agccaattcc tgctgcaatt gattgcccga tgcacttctt
    37561 tgtgaaaact caacaattcg cctgtcgctg aacaaactcg atgagctgat atggctttgc
    37621 aataccattc cttccaggtt agaacagcgg ctcaacgcca catatacctg gccgggtgca
    37681 aatgctgcac ctgcatcaat aattgctttt tcaaacgtaa ggccctggct cttatgaata
    37741 gtgatcgccc atgccaatcg taacggaaat tgggtaaaag aacccagtac atcagcgtcc
    37801 agctgccggg ttgttttatt taatgtatag cggatatttt cccatttttc tttctgcacc
    37861 tctatttccg gatcgtcatc gcattgcaca actatcttgt cggcttccag tttagctacg
    37921 gttccaatct ttccgttgaa atatcgtttt cctttttcag cagcatcgtt tctgataaac
    37981 attacctgtg cgccgacttt taaatgcaat acttcgtctg ccgggtatgc ccggtcagaa
    38041 aattcatcca ggacttcagc atcataagaa tacaacttgc cgctgagact gctcaactcg
    38101 tttgcattga tctcgctggc tttattatta tgtgtagtaa gaacgatata gccatcctgt
    38161 ttactacgcc tgaatgaggg ttgaaaccgg ctttccaaaa tgctcattcc ttccttatcc
    38221 agttcattgt ttcgaacctg gttcaggagg tcgataaatt gctgatcact ttgccggtat
    38281 attttattga attcaatata taccggcggg tcttcctgca gcaccttgct gtcgaagaaa
    38341 tagtgcccgc tatagtaatc agccaataaa ttccattcct gttccttgat gaccggtggc
    38401 aattgcaaca tatctccaat gaataaaagc tgcaccccac caaacctttc atcaggccgt
    38461 tgccttatat gacgaagaac cgtatcaatt gcatccagtg tatcacaacg gaccatactt
    38521 atctcatcaa tcaccagtaa ttccaattgc tgcaaaactt ttttcttgtc gctgttgaac
    38581 cgcaggcggc ttagcaggct atgcttattt gaaatttcgg ggttgctgct ttgaaatcct
    38641 gttccatttg ttgccggaat aaatggtgca agcggtaatt gaaagaaaga atgaatggta
    38701 acaccaccgg catttatggc tgccacacct gttggggcca ctaccgccag ctgcttgggg
    38761 caattttcac ggatatattt tagaaacgtt gttttaccgg tacccgcctt tccggtaaga
    38821 aatatattcc ggttactttg gttaactaac tgaatagcca gttgaaacat ttcattttca
    38881 gtgtcgtatt tcatcataca gtcattggca taaaaagaaa aggaacattg cgccagcctt
    38941 tgcctttgca atgttccgta ttatttgctg atttattact ccgcttttac accaagcatc
    39001 agcaactcgt taacctgtat ctcggtaacg tatttcttgt tcccttcttt atcattataa
    39061 ctacggttaa caagctttcc ttcgatagct acttcctttc ctttggtcag gaatttttca
    39121 acaatctcag ccactttacc ccaggcaacc aggttgtgcc attgtgtttc ggttactttc
    39181 tcgcctttgg cgttgcgata gttttcatta gtggctacag agaaacgggc cattttttta
    39241 ccactttcta atgttttcac ttctggtgcg ttacccaggt tgccgatcaa ttgtactttg
    39301 ttttttaatg cgtacataac tttaagtttt gtgcttccgt gcctttacgg ttttgcggaa
    39361 acgatttttg aattttgtta tctcagtcag ccggcgacgt catttccgat tgacaacaca
    39421 aagatgctaa tgccaaaaag cggtagccgg tttttacccg tttactcccg aagctaaacg
    39481 tgtagaaacg tttgtacacg gataaatagt tgtatattaa agttctaaac ctttaccaat
    39541 gatcaaggaa aaaacggata aacgaacctg cctggcctgt ggcaacgttt taaaagggcg
    39601 ggccgacaag aaattttgca acgattactg caggaataat tataataatg aacaaaaggc
    39661 gaaaggagga caaagtccac tggtgcgtaa tatcaataac gcattgctga aaaaccggaa
    39721 agtgctggag agcattcttc ccgaaggtga agaaaccgca aaagccaata aagacaaact
    39781 acaacggctg ggtttccagt tcaaatacct tacacatatt tatactacca aaacaggcaa
    39841 aacatattat tactgctatg attatggcta cctgccactg gacaatgatt ggtttttaat
    39901 tgtaaagaaa aaagaagaat agttctttgc ctttacattt gcagcatgtc aaatcctgta
    39961 atctgtatag gtgccgcatt agtagatgaa ttgtttcatg ctaccgataa tatgctgttt
    40021 gccactacca atgatgccac cgtaacaaaa actgcgggtg gtgtaagccg caatatctct
    40081 caccagttag ccttgctggg tgtacccgtt caactgatca gtgtttttgg agatgacagt
    40141 gatggtgact ggttaaaaca ggtttgtatt aatgccggag ttaaactgga tgcttctatt
    40201 acgaaaaaag ggctttccgg aaaatacacc ggtatcctga atgtggacgg atccttgttt
    40261 tctgcattct taaccaatgc ggccaatcat ttgatcacac cagagcattt aaccgggcat
    40321 attgagctgc taagaaccgc ttctttcctg ttagccgatg ccaatatcag tgttgatacg
    40381 gcggaatggt tacttgcgtt cagtaaagaa accgggatac cgtttgtgtt agaaccggtt
    40441 tcggttcccc ccgccaaaaa attcaccaac gttaacctgg atggcttgta tatgatcaca
    40501 cccaatgaag atgaattgcc ggtgctttgt tcagaacagg cttctatgac gcaaatgcag
    40561 atacagcaat tgctggaaaa aggagtacag aatatctggc tgcacaatgg caaaatgggt
    40621 tctgccttat acaacaaaga aaaagcaatt acacttcctg ctccttccct cgaagtggtc
    40681 gattgcaccg gtgccggtga tggttccctg tcaggcttta tactgggcaa acacctgggc
    40741 aaagaagaca tggattgtct taagctggcg catacattgt cagcagaaat attacaggta
    40801 aatggcgcta ttgccacaca cctggaccag gaaaaattat taggcctcat caccaaatac
    40861 tatccctcct gatgaattca ttattg
//
LOCUS       DAORTI010000016        38649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_645_length_38649_cov_6.105094, whole genome shotgun sequence.
ACCESSION   DAORTI010000016 DAORTI010000000
VERSION     DAORTI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 38649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38649
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_645_length_38649_cov_6.105094"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1104)
                     /locus_tag="PLO70_03950"
     CDS             complement(88..1104)
                     /locus_tag="PLO70_03950"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HQZ73639.1"
                     /translation="MPHLKKYKKMARSIAFRDALREAMSEEMRRDERVYLMGEEVAEY
                     NGAYKVSQGMLAEFGPKRVIDTPISELGFAAVGVGSAQNGLRPIVEFMTWNFAVLALD
                     QILNTASKMLAMSGGQISCPIVFRGGNGSAGQLGAQHSTAFESLYANIPGIKVVSPSN
                     GYDAKGLLKQAIRFEEDPVMFMESEQMYGDKSDIPDDEYYIELGKADVKKAGRDVTIV
                     SFNKMMKVALGAAAELEKEGVSAEVIDLRTIRPLDWMTILESVKKTNRLVIVEEQWPF
                     ASVSSEITYRIQKEGFDYLDAPIRRITAADAPLHYAPNLVAAALPDVSRTVKLVKEVM
                     YQVK"
     gene            complement(1218..2339)
                     /locus_tag="PLO70_03955"
     CDS             complement(1218..2339)
                     /locus_tag="PLO70_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ73640.1"
                     /translation="MNKAKIIFGFLPLLVAGFLLQDNSIIDGISSPSRYDGPYVLYKN
                     DKVYVNYIFDDKGVKTVQTDSVPVSEKNHLVLKIATDIPGKEFSVQLKSKLHNEKNDF
                     RKVSKQLAISDIEGTFSAFRKLLQGNNVIDDNFNWTFGDGHLVLTGDFFDRGDRVTEV
                     LWLIYSLEEQAKAAGGYVHFVLGNHEIMNLNGDLRYVHSKYMGNAGLLNENYLALYGE
                     QSELGRWLRTKNVMQKVGNVLYVHGGVSSVVNSMELSVSKVNELVRPFYGDTTYNYPD
                     LKVDVLYSDLGPFWYRGYYINKYKASQQQIDSTLFQFGVKYIATGHTIVADTISTSFN
                     KKLFNTDVPHYKGHSEALYIEKGSFYRVNATGEKFLIAE"
     gene            2668..2976
                     /locus_tag="PLO70_03960"
     CDS             2668..2976
                     /locus_tag="PLO70_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73641.1"
                     /translation="MVRYIMSFSIPDGRQQSAQKVIDLYFDTLYKNGPGGMRSQCYAD
                     EEDDCRFIHLKSFKKESVANHHFRSAIFREYIHQLEILCGNSLTFSKLIQQQTFESIY
                     "
     gene            3065..3850
                     /locus_tag="PLO70_03965"
     CDS             3065..3850
                     /locus_tag="PLO70_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ73642.1"
                     /translation="MHRVIIPVDFSETSLNAARYAAKMLAGKENAQAVLYHNYDSDDE
                     YDICLNYQESLKKEFLKAGVKNVVCEHEMGGDLIENISLLAHSIRATLVVMGITGKSA
                     IRQIMFGSNTLKLIDKNLYPVMIIPPDAVYKGVTHVAFATDFKNVELSTPSQLIGSVL
                     QLFDPKIHIVHVTKEKDILLSEEMQKEKEKLAQMFEHFRNEFFFITRNDFYEALDSFI
                     TEHNIDVLITVPKHQSNSSSLFKSTHTKRLAYHSHIPILAAHQ"
     gene            3926..4711
                     /locus_tag="PLO70_03970"
     CDS             3926..4711
                     /locus_tag="PLO70_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ73643.1"
                     /translation="MKTVIVPVDFSETSLNAARYAIKMLTGHYGVHMILHHVYEKTSQ
                     ASEANQRLESLKEELMTVGIVKITPLAEEGSDFRAVLEKLARLHQADLITMGITGRTS
                     IGQTIIGSNTLKMVEQKVCPVLIIPPDCTYSDVKNVLLTSSFKNISDSTPFVPIKKVL
                     KPFHPGLHIMNVDSEHYIAITEEYQAEQVKLKEMFSEFNPDFYFLSLHDIDEAINQFA
                     NDKKIDLIIIIHKEQTLFSKLFVKSHTKKLAYESNIPVLAVHE"
     gene            4939..5886
                     /locus_tag="PLO70_03975"
     CDS             4939..5886
                     /locus_tag="PLO70_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HQZ73644.1"
                     /translation="MKNIKIIEVPSEIGAGTRGASLGIEAIKIAALDFMSNFFIHFPS
                     EKIQTENKLLYEPIESPYAKRIKGIAAIYERMSKAVCDSMKNNFFPVVLSGDHSTSGA
                     TIAGIKMAKPKSKLGVIWIDAHADLHTPYTTPSGNVHGMPMAVSINKNNEDCAVHDID
                     DETAKHWNYLKNIGKIAPKVLPEDVVFISLRDYEKEEKHLIEKYDMKVITTKEVRSKG
                     AENVVRAVLRYLSDCTDIYISFDVDSLDSSISKGTGTPVSNGLKEREAEDLISKFMQN
                     RKVCCFEITEVNPTLDKENLMAEIAFNILQRSVNVLMMN"
     gene            5915..6544
                     /locus_tag="PLO70_03980"
     CDS             5915..6544
                     /locus_tag="PLO70_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HQZ73645.1"
                     /translation="MNTMNTSKGIFNIYKLSLLLFSLSGFSVVAQTGNYNKYIQEYQQ
                     AYSKNHEVVKGDDKKNIRFFPADESFRIQSSFERIYEAPWFKMETSGKEKKIYRVYGI
                     LRFTIKDTSLKLHIYQSQHLMSVKEYSNHLFIPFTDLSNGEESYENGRYIDLVIADVE
                     NGKYVIDFNKAYNPYCAYINNVYNCPVPPKENNLNVAIKAGEMKYGKKY"
     gene            complement(6639..7526)
                     /locus_tag="PLO70_03985"
     CDS             complement(6639..7526)
                     /locus_tag="PLO70_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ73646.1"
                     /translation="MKVLITGANGFLGHYLVSLLLQKGYEVIATGKGNNRLPFSNSEK
                     FVYTEMDFTNPFVVNDIFDTHKPEIVVHAGAVSKPDECELNQKEALRINTEGTVTMLS
                     NAAKHKCFFIFISTDFVFDGEAGMYTEVDSPNPVNFYGKTKLEAEEAVKRYNLDWAIV
                     RTVLVYGKPMAGRSNILTIVKEKLEKGEAYNVVDDQVRTPTYVEDLAAGIIAIMGKRV
                     CGIYHLSGINILTPYEMACKTADFLGLDKSLIKRVTAESFSQPAKRPARTGFIIDKAK
                     RELAYSPVSFLEGLKRTFS"
     gene            complement(7603..8319)
                     /locus_tag="PLO70_03990"
     CDS             complement(7603..8319)
                     /locus_tag="PLO70_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73647.1"
                     /translation="MKITYALKLFLGICLFAFTAPDLSGQNSTDTNTENSQAIFEDLQ
                     IVPMRLPVLQEVGGSLFLSQEYNPATIQIPSGKTISNIPVKFNIFNNAIMLQKDGQDM
                     KLESFRSVFYDQVQNDGTVKHFEFGSGYPEIDKQTEKTIYQVLSKGPKVHLLKYMSQK
                     VEDAATLGDYSRREIVTTEQFYIYVPGGAIKRIKGGKKDVAEALPDLSVKINEFVAAN
                     SLKLKSEGELTMLVEALNKP"
     gene            complement(8503..10644)
                     /locus_tag="PLO70_03995"
     CDS             complement(8503..10644)
                     /locus_tag="PLO70_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQZ73648.1"
                     /translation="MKRIFSLLLLLPFYAIAQVQYPVSSKGEVVDDYHGTKVADPYRW
                     LEDDWSNETKEWVTNQNKVTTNYLDQISFRKEWQSRLEDINNYPKYSSPSRKNEYFYY
                     SKNDGLQNQSVLYRQKGLAGKPELVLDPNKFSAEGTTSLAAFSISKNGKYAVVGKSAG
                     GSDWRTFFVMDLTTKKYLKDSLAWVKVSGASWQGDGFYYSRYPAPEAGKELSTKNENH
                     QVFYHKVGTSQDKDVLTYEDKENPQRFHSVFTDKSERYVFLNISDRGKGKEGNSLWYF
                     DNKSADKKFKPVIKEVGNYQYSFVEEVNGQFLLQTNDGAKNRKIVLVDPLNPAPANWK
                     TVIEEKEENMSSVTATGNKLFITYMKDVTSRVYQYDLNGNFEREVKLPALGTVSGFGG
                     EREDKFTFYTFTSFTFPPTIYKYDIATGQSTVFRKPEVKFNPEEYETQQVFYPSKDGT
                     KIPMFIVYKKGMQLNGRNPTILYGYGGFNISTTPSFSPARIAWLEQGGIYCLANLRGG
                     SEYGEKWHEAGMRFKKQNVFDDFIAAGEYLVEKQYTSNDYLAIQGGSNGGLLVGAVIN
                     QQPKLFKVAIPQVGVMDMLRFHKFTIGWNWIAEYGSSDNPDDFKNLFAYSPIHNIKSG
                     LNYPATLITTADHDDRVVPAHSFKYAATLQEHYKGENPVLIRIDVNSGHGASNLKKAL
                     ETTADIYSFTFYNMGLTPDFKNLKQAGKKAF"
     gene            complement(10754..11497)
                     /locus_tag="PLO70_04000"
     CDS             complement(10754..11497)
                     /locus_tag="PLO70_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73649.1"
                     /translation="MRKLLFYLLLLSSANSFAQSDSTKWLRAFPITAYMVDLDDTTKI
                     VQLEMPEGLIIKDKQPGIIYGVYRTAKDDAVEKGYGKCNLIKGQYYYFSIRNNKSGQP
                     LQKGDLLYTMMEKTNIYYGRIAQLAGHFIRLQNVYSDPFYDRYTLFSKWTANDEQKLI
                     DSVIMDIKFTGNYFINNDPSMNVFIKGGDYKGKRVLDVMTQCGPADIENFIDYMIARP
                     RLYAGKEWKISEIFATWLSEGAPTVIKNK"
     gene            11677..12660
                     /gene="pfkA"
                     /locus_tag="PLO70_04005"
     CDS             11677..12660
                     /gene="pfkA"
                     /locus_tag="PLO70_04005"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQZ73650.1"
                     /translation="MEKKVTKIGVLTSGGDAPGMNAAIRAVVRTGIFYGLEVYGIMRG
                     YQGMIEDDIIKMDGRSVANIIQRGGTILKTARCKDFFETEGRKKAYNNLKKRGINGLV
                     IIGGDGSFRGAVKFSQEFDIPCVGLAGTIDKDISGTDFTIGFDTAVNTAVEAIDKVRD
                     TMDAHDRIFIIEVMGRDAGYIALHSGIATGAENILIPERKTDMKNIITSLQEKERRKK
                     LVNLIVVAEGDDFGGANEVQKILQESLPKAEIRVCILGHIQRGGSPSCMDRLIASRMG
                     YHAVESLIEGRHNVFVGIQNNKMNYIPLETAVKNKGKISDEWLKIVKILAS"
     gene            12701..14191
                     /gene="pyk"
                     /locus_tag="PLO70_04010"
     CDS             12701..14191
                     /gene="pyk"
                     /locus_tag="PLO70_04010"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQZ73651.1"
                     /translation="MTKNIDKYLHKEFDKEAGRQHAYKRTKIVATVGPACDTYEKLLE
                     LVKAGVNVFRLNFSHGEHEDKTKIINFVYQINKTEPYNIAILADLQGPKLRVGDLEGG
                     ALEINDGDILTFTNEKVMGTKEKIFVSYPDLHSDVKVGEKILIDDGKLEVIVREITKD
                     KEVKVEVTLGGTLYPKKGVNLPDTKISLPSLTEKDLNDLNFILKHRIDWVALSFVRNP
                     EDIIDLKRRIKQAGHKAKVIAKIEMPQAIINLRDIIVESDGIMIARGDLGVELPIEEV
                     PLIQKDIIKKCLHRAKPVIVATQMMESMIDRTKPNRSEITDVANAVLEGADAVMLSGE
                     TATGKYPKLVVETMTKIILEIEAKAYDYNREEMLRPQGHSPSFLSDAICYNACKLAED
                     VNADAIIGMTQSGYTGFMLSSYRPKSLLYIFTKERSLVNQLSLSWGVRAFHYDEEESL
                     DDIFSDQIDILKERSFLKNGDVVVSTGSTPVHLHLPTNVLKIAKVE"
     gene            complement(14280..15056)
                     /locus_tag="PLO70_04015"
     CDS             complement(14280..15056)
                     /locus_tag="PLO70_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQZ73652.1"
                     /translation="MKFFLVCSIVLLSIIACNDNDATGEATDSTTITAKEGLGMNVLT
                     DQQKTEGWQLLFDGESTKGWHKYGGKPVGSAWKVADGALYLDTTIKENWQIKDGGDIV
                     SDSAFENFHLKLEWKIAKDGNSGIMFYVHEDTVKYKWPWETAPEMQVLDNNGHPDSKI
                     IKHRAGDLYDLISCSKETVKPAGEWNLAEVKCLNGKLDLFLNGENVVSTTLWDDNWKK
                     LVAGSKFVEMPDFGTYKKGKIGLQDHGNAVWYRKIMIKKL"
     gene            complement(15062..15451)
                     /locus_tag="PLO70_04020"
     CDS             complement(15062..15451)
                     /locus_tag="PLO70_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ73653.1"
                     /translation="MVKKNIISFFLLMILVACAGNSKEGKNESGTDETAISASNDPVY
                     QKGLELVARNNCLTCHKVDDVFTGPSYRQIAKRYKGASAAEVAELAQKIIKGGNGMWG
                     SAMMTPHPGISRQEAEAMVQYILLLKK"
     gene            complement(15499..16554)
                     /locus_tag="PLO70_04025"
     CDS             complement(15499..16554)
                     /locus_tag="PLO70_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQZ73654.1"
                     /translation="MTTIKGPGIFLAQFMGDKAPFTNLKSICLWAEAIGYKAVQIPTW
                     DKRCIDLQLAAESKTYADEIRGIVNDCGLEISELSTHLQGQLVAVHPAYDALFDGFAP
                     EEYRGNAKARTEWAVQQVKYGAKASQHLGLNAHPTFSGSLLWHTFHPWPQRPAGLVEE
                     GFKELGKRWTPILNYFDECGVDVCYEIHPGEDLFDGITYELFLKEVNNHPRACLLYDP
                     SHFVLQQLDYIQYIDFYHERIKAFHVKDAEFNPTGKQGTFGGYQNWANRAGRYRSPGD
                     GQVDFKTIFSKLTQYDYSGWAVMEWECCIKHPEDGAREGAEFIQRHIIRVTERAFDDF
                     ASSGTDEKANRLLLGLD"
     gene            complement(16585..17745)
                     /locus_tag="PLO70_04030"
     CDS             complement(16585..17745)
                     /locus_tag="PLO70_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73655.1"
                     /translation="MVLRKLRMGMIGGGPGAFIGAVHRIAANMDGEIELVCGAFSSDP
                     ERSAEMGKALYLSPDRVYGSYKEMIRREAALPEHQKMDFVSIVTPNHLHFQPAKLALE
                     NGFHVVLDKPMTFDLKEAKELKEIAAKSGNYFCLTHTYTGYPMVKEARQQVLSGKFGV
                     IRKVYVEYPQGWLSTFLEGANQKQAAWRTDPSRSGIAGSMGDIGTHAFNLAEYVSGLK
                     TTKICAAINVVVEGRKLDDDGAVLLEYDNGASGVLIATQVAAGEENNIRIRVYGEKGG
                     LEWKQEDANSLIVKWLDKPAEIWRSGNSYLSSFAQHNTRTPAGHPEGYLEAFANLYRN
                     FSLCVKAKMEGVEPKEEWLDFPGADEGIRGMVFIEQVIASAKSKEKWIDFIV"
     gene            17897..19108
                     /locus_tag="PLO70_04035"
     CDS             17897..19108
                     /locus_tag="PLO70_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HQZ73656.1"
                     /translation="MKTTIRIQLSTMMFLNFFIWGIWFVTMGTYLGSKLSADGVQIGL
                     AYGTQSLGAIIAPFIIGLIADRYFAAQKILGILHLAGASLMYYLSTVKDFTSFYPVLL
                     VYMIIYMPTLALVNAISFKQMSNPEKEFSFIRVWGTIGWIIAGLLIGWLALEKKGSLQ
                     NTFMIAAIVSGALSLFSFALPNTPPPKAGTKASFGEIVGLDALGLLKDKNFLIFFLAS
                     MLICIPLAFYYQETNIFLNEVGVQNAAGKMTMGQMSETIFLFLMPLFFLRLGVKKMLL
                     IGMLAWVIRYLCFGFGDAGSGVMLLYAGIILHGICYDFFFVTGQIYTDQRAGDKIRSS
                     AQGMITLATYGVGMLIGFWFAGFIAEYYKTADGHAWKNIWMVPAAIAGVILVLFFLLF
                     KDPGKKEKMES"
     gene            19130..20023
                     /locus_tag="PLO70_04040"
     CDS             19130..20023
                     /locus_tag="PLO70_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQZ73657.1"
                     /translation="MSQKKTRRELLKQLTAGSLAVTALPSMSSFVSNSEYEYSLKGNI
                     NHSVCAWTYNFLPLDDMCSLVKKLGFTAIDLLAPKDWSTIQKQGITCSMCYTAGKISL
                     TEGWNKKDNHDWLIKDFTEAIPLVAKAGYKNLICFSGNRNGMNDDTGLKNCAEGLKQI
                     MLLAEQNNVIVQMELFNSKIDHKDYMADKSAWGVELCRKIGSPNFKLLYDIYHMQINE
                     GDVIRTIRDNHQYFGHYHTAGVPGRHEIDESQELFYPAIMKAIAATGYKGYVAQEFIP
                     TGKEKKEKIAALKKAIKICDV"
     gene            20062..21741
                     /locus_tag="PLO70_04045"
     CDS             20062..21741
                     /locus_tag="PLO70_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQZ73658.1"
                     /translation="MADNLYDAIVIGSGISGGWAAKELTEKGLKVIMLERGRNIEHIK
                     DYVSATKESWEFPHRGGRTQQMIKDYPVLKRDYPLNETVLDLWCKDSEHPYTEVKRFD
                     WYRGYHVGGRSLTWGRQSYRFNKWDFEANAKEGIAVDWPIRYDDLAPWYSYAEKFAGI
                     QGNKDGLEVLPDGDFMPAMELNCVEKDIKKRLETYYKNTRHLIIGRSANITQPHHDRT
                     NCQYRNRCWRGCPFGGYFSTQSATLPAAMKTGNLTLRPFSIVTKILYDKDKKKATGVE
                     ILDAETNMTYEYKAKIVFVCASALNSTWVLMNSATNIWPDGLGSSSGELGHNVMDHHF
                     RCGASGTVEGFSDQYVYGRRPTGIYIPRFRNIYTDKRDYIRGFGYQGGAGRGRGVEVA
                     EFTIGADLKDALSIPGDNWGMGITAFGEMLPYHENKIALDKEKKDKWGLPVLAFDVEI
                     KENEKKMRIDMMNDAKEMLEAAGVKKVESYDHGYAVGMGIHEMGTARMGRDPKSSVLN
                     GNNQVWDAPNVFVTDGACMTSASCVNPSLTYMAMTARAANFAVSELKNGNI"
     gene            21761..22399
                     /locus_tag="PLO70_04050"
     CDS             21761..22399
                     /locus_tag="PLO70_04050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQZ73659.1"
                     /translation="MERRELLKLIALLTGGAVVGGEVFLAGCKTAGKADTGFTTSTIA
                     LLDEIGETIIPATVTPGAKAAKVGEFMKVMVTDCYTEKQQAAFTNGIAQLEEACKKAT
                     GKSFMECMPEQRHDFLVSLEKEAKEFNNKRNEEDKPKREAHKAENEKLAFKDQKEFEG
                     TPSHYYTMMKQLTLLGFFSSRTGMTETLRHLPVPGKYDGAYPYTKGDKAWAE"
     gene            22426..23304
                     /locus_tag="PLO70_04055"
     CDS             22426..23304
                     /locus_tag="PLO70_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQZ73660.1"
                     /translation="MDNSRRKFIKITSGVTTGFALSGLSGLSYFSSCEETGKLKKFGI
                     QLYTLRADMPKNTKGVLKQLASFGYRQIESYEGPQGMFWGMSNTEFKKYMDDLGMSIV
                     SSHCDITKDFEKKAAEAAAIGMRYLICPWKGPQKSLDEFKKFTDEFNQKGEICKKNGI
                     RFAYHNHDYTFKLLEGQIPQDVLMTGTDASLVDFELDMYWVAAAGEDVEAWLKKYPNR
                     FRLCHVKDRSKTPGTDNGKNSVDLGTGSMNWSKIFSTARANGMEYFIVEQEAYPNGTA
                     LEAAKVNAEFMKGLKI"
     gene            complement(23309..24046)
                     /locus_tag="PLO70_04060"
     CDS             complement(23309..24046)
                     /locus_tag="PLO70_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HQZ73661.1"
                     /translation="MNEVRYIIEIATSDFATTQSAVQGGADRIELCANLAEGGTTPSY
                     GTIKKCREAFPVLLYPIIRPRGGDFLYTSEEFEIMLQEVKLCRQLGCDGVVIGLLNTD
                     GSIDVKRTAQLVEAAYPLGVTFHRAFDRCRNPFEALEQLIVTGCERILTSGQKPAAPD
                     GVKLINELNREANDRIIIMPGSGVRKENIKMLAQETGCTEFHSSLRGKEKSKLDFIHP
                     AFADSAESYSNNAISPEEVRALRSSLY"
     gene            complement(24039..25094)
                     /locus_tag="PLO70_04065"
     CDS             complement(24039..25094)
                     /locus_tag="PLO70_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQZ73662.1"
                     /translation="MVNRRKFIHASAFGSLGILFSKMAAGNQLPFTIKGKPVVISTWD
                     AGLEANKAAWEVLFKGGRAVDAVEKGVMVTEASQNCCVGLGANPDRDGFVTLDACIMD
                     EFSNCGSVAFLERIKHPVAVARRVMEKTPHVMLVGSGAQQFAVTEGFPLEEQKLSKQA
                     QAAYEEWLKNSEYKPPVINIENLPVRQAGEQGHGPFAPLKLSNGEWNHDTIGMVAMDT
                     HGNMGGSCTTSGAGFKMRGRIGDSPIIGAGLFVDNEVGACTATGQGEDVIRVAGSHSV
                     VELMRQGLSPEAACKKVIERIVKIKKAKAKDIQVAFLALNKKGQAGAFALHPGFKYAI
                     KSGTKETLVTSKSWFNE"
     gene            25230..25787
                     /locus_tag="PLO70_04070"
     CDS             25230..25787
                     /locus_tag="PLO70_04070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQZ73663.1"
                     /translation="MKIEIISGSPRTNSVTKRVALHLKNWMLQQTNHEIDMIEMKDWD
                     VPPVQSVWVSVDKAPVELQPLAKRIFEADAFILVTPEYNGSYSPAMKNLLDHFPKQLH
                     KPFGIVTASPGAMGGMRASQQLLLLVPALFGIASPNMLITPSVDKKFSPDGDLLDESF
                     QNAVHTFITEFLWLSEKLVTEKVIA"
     gene            25803..26513
                     /locus_tag="PLO70_04075"
     CDS             25803..26513
                     /locus_tag="PLO70_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQZ73664.1"
                     /translation="MKTILHKANTRGHADHGWLNSFHTFSFAGYYDPNRTHFGVLRVL
                     NDDTVAGAMGFGAHPHDNMEIISIPTSGDLEHQDSMGNKQVIKQGDVQVMSAGTGVTH
                     SEKNKNQDKEVKFFQIWVFPNKKNVVPRYDQKNFSAADKHNRLLTVVSPIGSADGGVQ
                     IHQDAWFSLGNLDKDFRVSYQLKNKNNGVYAFVIEGEVTINGEKLSRRDGLGITETDQ
                     LNIQADSAAELLLMEIPV"
     gene            26522..27400
                     /locus_tag="PLO70_04080"
     CDS             26522..27400
                     /locus_tag="PLO70_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQZ73665.1"
                     /translation="MKNKRSIEHIIAPPPPHMVGDGFRVHSFFPGAKLIDKKRMSPFF
                     LMDYGSKIEFAPSDEPRGVGVHPHAGFETVTIAYHGKIAHHDSAGNSGIIGEGDVQWM
                     TAGAGLLHKEYHEKEFSKTGGPFQMVQLWVNLPSQYKKTKPKYQEITNAMMGRHYLSD
                     EKSVVEVIAGDYNGTKGPASTFTPMHVYNAKLKKGASTILNFPSHYNTGMLIVDGNAM
                     INDQAVPKDHFVLFKHDGEIITIEATEDAVILVLSGEPINEPIAAYGPFLMNSWPEVE
                     QAIQDVNMGKFGVLED"
     gene            complement(27424..27915)
                     /locus_tag="PLO70_04085"
     CDS             complement(27424..27915)
                     /locus_tag="PLO70_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ73666.1"
                     /translation="MQTNTAIISRFYTAFQQLDSRTMNSCYSDDIVFNDPAFGLLRGE
                     EAKSMWEMLCKNARDFSLTFSNIQLLDEEYATCNWTATYTFSKTGRKVVNHIKAYMKL
                     KDGKIIEHSDAFKLSEWAAQALGWKGALFGWTGFMKKRIQNNARKSLIAFIEKKDAGS
                     GPA"
     gene            27974..29863
                     /locus_tag="PLO70_04090"
     CDS             27974..29863
                     /locus_tag="PLO70_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQZ73667.1"
                     /translation="MKKIIITILLWFSVFIVIAQSDPQDSAWIKDNYTKLEQYIPMRD
                     GIKLFTAIYIPNDKTEKHPILVTRTPYSCAPYGKEFSSRLWERHWKYYARENYIIVVQ
                     DVRGRWMSEGEFEDVRPFNKNKTGLETDEASDTYDAIDWMVKNLPGNNGNVGVFGISY
                     PGFYSTMAAASGHPALKAVSPQAPVTDWFMGDDFHHNGAFFQLDGFGFYSGFGKPRPK
                     PTTIGSAGFNFKVRDNYKFFLEAASLKKLAALMGDSIQFWKKMYAHPNYDTWWQARDA
                     RRAMYNVNPAMLVVGGLFDAEDCYGAWNLYKAIEKQNPSANNKIVMGPWSHGQWSSGD
                     GSFLGNVKFGMNTAHWYQNNIEIPFFNYYLKGKGEVEKIPEATIFFSGENQWKKLNQW
                     PPAAMQSKDIFLTENGGLSWTAPAGKESFSAYTSDPAHPVPYTEDVHHGRTREYMTDD
                     QRFAARRPDVLTFQTDVLTEDITLAGVVTANLKVSISGTDADFVVKIIDVFPDDFIYP
                     GDKPQAGRTAGGSYPMGGYQMMVRGEIMRGKYRNSFETPTPFKPGKVETVRFDLPDVA
                     HTFKKGHRLMIQIQSSWFPLADRNPQQFMNIYEANETDFKKAVIRIFHDAKNQSSIVL
                     PVMNK"
     gene            29870..32218
                     /locus_tag="PLO70_04095"
     CDS             29870..32218
                     /locus_tag="PLO70_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQZ73668.1"
                     /translation="MRFLICFLFALSASTVTIAQQKQLALIPYPVEVTQGTGYFTIKN
                     TVSVSSSLPGHEWKYLFSYFKDEMKKQFGIAVKEVTKGQPADISFNMRRMPTSGKAAY
                     QLEVTKNGVIINSNFAAPAFHAIQTLFQLFPVDKKVPGQIPVVKIFDHARFEYRGMHL
                     DVSRHFFPVDFIKKYIDFLAYHKLNTFHWHLTDDQGWRIEIKKYPGLTEIGGWRNGTI
                     IGRYPGTGNDNISYGGYYTREEIKEVVQYAKERFIEVIPEIEMPGHSSAAIAAYPWLS
                     CFPEKPTLIPANMISQKSIEEQKKGRVKLVQETWGVFDDVFCAGKDSTFIFLQNVVDE
                     VIELFPSKYFHIGGDECPKAHWKKCVHCQKRMKENNLKDEHELQSYFVQRMEKHLNSR
                     GKILIGWDEILEGGLAPKAVVMSWRGEAGGIEAAKQKHNVIMTPGNPVYFDHTQSENE
                     DSVTIGGYNPIEKVYAYEPIPKELNEEEGKYVLGAQANLWTEYIDNIKKVEYMIFPRM
                     AALSEILWSPKEKKNWDSFEKRLLTQFNRYEKWDANYSKAYFDLKSYVLPATNHSGII
                     WKVESKLKNDSIFFKEAKGLPKPIKYTEPVLISETSNKHVFQIFNGNKTSSITQQFNI
                     NKATGKKITLTTPPSKTYPGDGAFTLVNGVQNEKGTAKSKEFLGFSGTDCEAIIDFGK
                     EESINSIKIHVLDQQSSWIYPPAWMKVYYSADGINFTEALNAEAGKEVPGEIIKDIEW
                     SFYPVSKTRYLKVFLKNFGTIPEGKPGAGNKAWLFVDEIEVN"
     gene            32290..34218
                     /gene="nagB"
                     /locus_tag="PLO70_04100"
     CDS             32290..34218
                     /gene="nagB"
                     /locus_tag="PLO70_04100"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQZ73669.1"
                     /translation="MSKKAASTNFADSFEKIPVNIFPDQKEGSVFVANQIAAFIREKQ
                     QKKEKCVLGLATGSTPKTLYTELVRLHREEKLSFKNVITFNLDEYYPIDNNALQSYNR
                     FMKTHLFDHVDINPKNIHIPNGEIKKENIKEHCKQYEKMILDAGGIDLQILGIGNNGH
                     IGFNEPGSGIYSKTRLITLDNSTRIANAYEFANISEVPRLAITMGISTILKSKKVILM
                     AWGSAKAPVIKKAVEEDDSEQVPASLLQNHDDVTFVVDDSAAAELTRNKSPWLTGDCE
                     WTPKIIKKAVVNMALKLKKPVLSLTNSDYNEYGLSDLLVEKGDAYEINLQVYYMLRDS
                     ITGWPGGKPNAYIPAHPERSSPYPKKVIIFSPHPDDDIISMGGTFQRLHDQGHEVHVG
                     YQTSGNIAVTDEFVTRFLDFAVGFEEIAGIDTSKSGKILDNARKFLARKKSNQVDTPT
                     IRSIKGLIRQGEAKATCRYVGIKDENIHFQNLPFYETGTIEKKPMSEKDVKITIELLR
                     KIKPQQVYCAGDFADPHGTHIVCFNVVLEALKRIKNAGDEWISDCWLWLYKGAWQEWN
                     IEEIEMAIPMSPDQVLKKRFGIFIHQSQKDMVPFQGSDSREFWQRAEERNAATANLYA
                     DLGLTHYAAMEAFVRWHY"
     gene            34273..35028
                     /locus_tag="PLO70_04105"
     CDS             34273..35028
                     /locus_tag="PLO70_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQZ73670.1"
                     /translation="MSIFKSSNPTLQEKTYQGTILEGISTGEEMTLKGTMNKFGVLVM
                     LMIGSTLFAWSQFYKGSDPKIFMMVGVFGGLALALVMAFKMKWSTFLAPAYAILEGLF
                     VGSISAMYDYMYPGLPLQAVALTLLVTLIMFLIYRYRIIKVTQKLRTVVVVATAAIAV
                     FYLVQWLTYSFSSTAVAYSFTNSSSPISIGFSILVSGLAAFNLLLNFDTVEKGVEMRA
                     PKYMEWYSAFGLLVTVVWLYLELLRLLSKFNRN"
     gene            35141..35217
                     /locus_tag="PLO70_04110"
     tRNA            35141..35217
                     /locus_tag="PLO70_04110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35175..35177,aa:Arg,seq:acg)
     gene            complement(35350..37371)
                     /locus_tag="PLO70_04115"
     CDS             complement(35350..37371)
                     /locus_tag="PLO70_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ73671.1"
                     /translation="MQFMVLQLSIIWKKFVFCFPLFFISLLIFSQPATTDIPGDTTRV
                     NSLLQQSKENFTNDPDKAISLANEAKALADRIDFQKGTALALKNIGIVYYFQGKHLEA
                     LDYYTQSLEVFKIIGDNSGIANLYSNIGVVYYDRGDDAKALENYLQSLKFSELAGDKF
                     RILIALNNVGGVYNIKPATYDKALEYYLKALSISEELGKMEESGTICVNIGTIYFEKD
                     NDEQALFYFNKSLKAYGDSEGSLNAYNGLGKIYRKEENYELALQNHQKALALAEKLNN
                     KISIVQSLMGLANVYVKKGDYPTALQYFRKAELPALEIHANHEIKDLYQEMALAYSKT
                     KDYSNAFKYQTLFSSIKDTLYNIDTDKKLGTLQFDFDLQKKQGEINLLTKDMALTDIK
                     LKRQQFTKKVLIVGLILVLLIALLIFRNYRIKVRTHKILDRQKGEIESLLLNILPSEV
                     ANELQVYGHAIPRNYEFVSVMFTDFKGFTAIADKMSPEELIQELNTCFVAFDDIIGKY
                     GLEKIKTIGDSYMCAGGIPTADEHHVENMVKASLEICHFIRQYNQKRNLEGLESWDLR
                     IGVHVGPVIAGVVGKKKYAYDIWGSTVNIASRLESNGMPGKVNISSSVFELIKDKYSC
                     SYRGKINAKNIGDIDMYFLDGMAVIEGDETGINTNENSKTVPDLQGQEK"
     gene            complement(37411..>38649)
                     /locus_tag="PLO70_04120"
     CDS             complement(37411..>38649)
                     /locus_tag="PLO70_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HQZ73672.1"
                     /translation="NNVGTYDYTLGNLSAGTNYSLLLSSVAPVPTFAITIKSVTITPV
                     PGQSKVYGTSTDPVFTFTNNAGLSAFDFTGALSRVSGNNAGSYAYTLGNLSAGSNYSL
                     SLSTVVPIATFVITKAPLQVKADLKWINKGSPLPTFTSTITGLKNGDNPTVSYSLSPS
                     CTGSAGVYTIIPLLKPFANSINYTITYTNNYLYINPKGSGADDVDTYLECVEDRGASY
                     MPANRRYVARFYAKNTNSTPVYVPIGVNNSLSSAGSFDGSQQPVVFLPGNGTTRFNVP
                     FDGVTLKWQLKTYEGNYLKTESVTASSSSVKCAINYAPAARGATDESNYEEPPTTAKT
                     SLYPNPATNRVIIITAESVQSEKGIFLYDVYGRYYPVKVIRRISEHSFELDVSGLLNG
                     VYLIRVRGENGYQTLRFVKG"
BASE COUNT        11889 a         7975 c         7492 g        11293 t
ORIGIN      
        1 taagtttttt ttctcaattg ctataattgg tgccataaac aaaagccccg cacagtgcag
       61 ggcttaaaaa aatacaatta tttaaagtta cttcacctgg tacattactt cttttacaag
      121 ttttaccgtt ctggacacat ctggcaatgc ggctgcaacc aggtttggag catagtgcaa
      181 aggcgcatct gcggctgtta tccggcggat tggcgcatcg aggtagtcaa atccttcctt
      241 ttgtatacga taggttattt cagaagaaac agaggcaaat ggccattgtt cttccactat
      301 taccagccgg ttggtctttt ttacgctttc taatattgtc atccagtcaa gcggccggat
      361 tgtcctgagg tctatgactt ctgcactcac cccctctttt tccaattcag ctgcagctcc
      421 caatgccact ttcatcattt tgttgaaaga aacaatggtg acatccctac cagccttctt
      481 tacatccgct tttcccagtt cgatataata ttcgtcatca ggaatatcac ttttgtcgcc
      541 atacatctgt tcactttcca taaacattac cgggtcttct tcaaaacgta tggcctgctt
      601 cagtaagcct tttgcatcat acccattgct gggagaaacc actttaattc caggaatatt
      661 tgcatacaaa gactcgaaag cagtggagtg ctgagcaccc aattgtccgg ctgaaccatt
      721 gcctccccta aatactatag gacaagaaat ctgtcccccg ctcattgcca acattttaga
      781 agcagtattt agaatttgat ctaaagctaa aacggcgaag ttccatgtca taaactcaac
      841 aataggacgc aaaccattct gggcagaacc aacaccaaca gctgcaaaac ccagttctga
      901 aataggtgta tcaataactc ttttaggccc aaactctgcc agcattccct ggcttacctt
      961 gtaagctccg ttgtattctg ccacttcctc tcccatcagg taaacccttt catcccttct
     1021 catttcctca ctcattgcct ctctaagggc atcccgaaat gcaattgatc ttgccatctt
     1081 tttatacttt tttaagtggg gcaaatttaa gcgacaggcc gcataaaacc taaaatcaaa
     1141 aagccctcaa agggcctgct ttcaaaggaa tttactgcag ttattacttt taaaactatg
     1201 tgcctgataa actgggatca ttctgctatc aaaaactttt caccggtcgc atttaccctg
     1261 tagaaactgc ctttctcaat atacaaggct tcagagtgcc ccttataatg agggacatcg
     1321 gtattaaaca gttttttatt aaacgaagta ctgattgtat ctgctacaat tgtatgccct
     1381 gtagcaatat attttactcc aaattgaaat aatgtactat caatctgctg ttggctagct
     1441 ttatacttat ttatataata ccccctgtac caaaatggtc caagatcact gtacagaaca
     1501 tctaccttca ggtcagggta attataagta gtatccccat agaaaggcct taccagctca
     1561 ttgaccttag aaacggatag ctccatacta ttcactaccg aagaaactcc tccatgaaca
     1621 taaagcacat tccctacctt ctgcataaca tttttggttc tgagccatct tcctaattcc
     1681 gactgctctc catacaaggc caggtaattt tcatttaaca gaccggcgtt tcccatatac
     1741 ttggagtgca cataccttaa gtccccatta agattcatta tttcatgatt acccaataca
     1801 aagtgaacat agcctccggc agcctttgct tgttcttcca gtgaataaat aagccagaga
     1861 acttctgtaa cccggtcccc cctatcaaaa aaatcgcctg tcagtacaag atgcccgtca
     1921 ccaaatgtcc agttaaaatt atcatctatc acattattgc cctgcagcaa ctttctaaaa
     1981 gcactgaatg ttccttctat atccgatatg gccaattgct tactgacctt cctgaaatca
     2041 tttttttcat tatgcaattt agatttgagt tgaactgaaa attccttacc gggaatatcc
     2101 gttgcaattt ttaatactaa atgatttttt tctgataccg gaactgagtc tgtttgtacc
     2161 gtcttgacac ctttatcgtc aaaaatatag ttcacataca ccttatcatt tttatacaat
     2221 acataaggcc cgtcataccg ggagggtgaa gaaattccat ctataattga attatcctgc
     2281 agcagaaacc cggctaccag cagagggaga aatccaaaaa ttatttttgc tttattcatt
     2341 ataaccggtt tgagggatta ctgaattaaa tgacgaaaca atcgctgcag cagttgcgca
     2401 aataaccctg tttaaagata ttgcaaaaaa attaaccaac agttttgcag gtgaggagaa
     2461 ataaccctgc attgtttttt gactccgttt aactgaaatt tgcctccaac cccttaactg
     2521 taaaccagca ttgactttta cttataagca caaccataat ttaccgctca caggatattt
     2581 actgcactgg taacaaccag gcagcatttt atgaagaaaa gttgccatac aaataccggc
     2641 tgtactaaac caaaaaccaa atgctttatg gtaaggtata tcatgtcctt ctctattccc
     2701 gatggccggc agcaatctgc tcaaaaagta attgatcttt attttgacac tttatataaa
     2761 aatggcccgg gcggcatgcg gagccagtgc tatgcagatg aagaggatga ttgtaggttt
     2821 atacatctta aaagtttcaa aaaagaatct gttgctaatc atcatttcag gtcagccata
     2881 ttcagggagt atattcatca attagagata ctttgcggta acagccttac cttctccaaa
     2941 ctaattcaac agcaaacatt tgaatcaatt tattagtgaa cataggtaaa tgtttaaaaa
     3001 gtgtctgttt tgaaacttta aatccattaa atttatcttt tcgaaacaaa acaaccaatc
     3061 aattatgcac agagtcatca ttcctgttga cttttccgag acctcattga atgctgcccg
     3121 ctatgctgca aaaatgcttg cagggaaaga aaatgcgcag gctgttttgt accataatta
     3181 cgacagtgat gacgaatacg atatttgtct taattaccag gaaagtctta aaaaagagtt
     3241 cctgaaggca ggtgtaaaaa atgtagtatg tgaacatgaa atgggtggcg atcttattga
     3301 aaatatatcc ctcctggcac atagtataag agctacactg gttgtaatgg ggattacagg
     3361 taaatctgca atcaggcaga tcatgtttgg aagcaatacg ctgaaattga tagataagaa
     3421 tctttacccg gtaatgatca tccccccgga tgctgtctat aagggtgtaa cccatgttgc
     3481 atttgccact gactttaaaa atgttgaact gagcacccct tctcaattaa ttggctcagt
     3541 attgcaatta tttgacccta aaatccacat cgtacatgta actaaggaaa aagatatttt
     3601 gttatcagaa gaaatgcaaa aagaaaagga aaagcttgca caaatgttcg agcattttcg
     3661 aaatgaattc ttctttatta cccgcaatga tttttacgaa gcattggata gttttattac
     3721 tgaacacaac atcgacgtac tgatcacggt acccaaacat caaagcaatt cttcctccct
     3781 ttttaaatct acacatacaa aaaggctggc ctatcacagc catattccga ttttagcagc
     3841 ccatcaataa ttcactccgc tcactatacg ttagataaat tattttttat ctttatgaaa
     3901 taccggaata gtaaaaaaaa accatatgaa aactgttatt gtacctgttg atttctcaga
     3961 aacctcactg aatgcagccc gatatgctat aaaaatgctg acagggcatt atggtgtaca
     4021 catgatcttg catcatgtgt acgaaaagac aagccaggcc tctgaagcaa atcaacgtct
     4081 ggaatcatta aaggaagaat taatgaccgt cggtattgta aaaatcactc cactggctga
     4141 agaaggaagt gatttcagag ccgtactcga gaaactggcc cgcctccacc aggctgatct
     4201 gattaccatg ggaattactg gcagaacatc tatcggccag acaatcatag gcagcaatac
     4261 tttgaaaatg gtagaacaga aggtatgccc ggtgttaatt attccaccgg actgtactta
     4321 tagcgatgtt aaaaatgttt tacttacatc aagttttaaa aacatttctg acagtacacc
     4381 atttgttcct ataaaaaaag tactcaaacc cttccatcca ggcctccata taatgaatgt
     4441 ggacagtgaa cactatatcg ccattacaga agaataccag gcagaacagg tcaagttaaa
     4501 agaaatgttc agtgaattta acccggactt ctattttcta agcctgcatg atatcgatga
     4561 ggcaattaat caatttgcga atgataaaaa aattgacctg attataataa tccataaaga
     4621 acaaaccctc ttttcaaaac tttttgtaaa aagtcatacc aaaaagctgg cctacgaaag
     4681 caatattcct gtattagctg ttcatgaata atgaattaaa tgcaggaaaa cggtacttga
     4741 cagcctgcaa actaaaggct atctttacaa aagtatcttt atgaccctgg taaataaaat
     4801 actgacttgt attaatactt ttgctgagca atcactgcct gcaaatttct atatccctga
     4861 ttaagtagcc gcgtctttta ctaattaacc tattacacta ccggccttta ccggaaatca
     4921 tattcaactg ttgccagtat gaaaaatatc aaaattattg aagttccttc tgagattggt
     4981 gcgggcaccc gtggagccag cctgggtatt gaagcaataa aaattgcagc acttgatttc
     5041 atgagtaatt ttttcattca tttcccttca gaaaaaattc agacggaaaa taaattgcta
     5101 tacgaaccga ttgagtcacc ttatgccaaa cgcataaaag gcattgccgc tatttatgaa
     5161 agaatgagca aggccgtatg tgactctatg aaaaataatt tttttccggt tgtactcagt
     5221 ggtgatcata gtacatctgg tgccaccatt gccggaataa aaatggcaaa gcccaaaagt
     5281 aaactggggg taatctggat agatgcacat gcagacctgc atactcccta tacaactcct
     5341 tcaggcaatg tacatgggat gccaatggcc gtttcaatta ataaaaacaa tgaagattgc
     5401 gcagtgcatg atatagatga tgaaacagca aaacactgga actacttgaa aaatatcggc
     5461 aagatcgctc cgaaagtttt accagaggat gtagttttta tttcactgag agattatgaa
     5521 aaagaggaaa aacatctcat agagaagtat gatatgaaag tgatcactac aaaggaagta
     5581 cgaagcaaag gtgctgagaa tgttgtcaga gctgtgctcc gctatttatc agactgtact
     5641 gatatctata tttccttcga tgtggacagc ctggattctt ctatatccaa aggaaccggc
     5701 acaccggtaa gtaatggact caaagaaagg gaagcggagg atcttatcag taaattcatg
     5761 caaaaccgta aagtgtgttg ttttgaaatt actgaggtaa accccacatt ggataaggaa
     5821 aacctgatgg cagaaattgc gttcaatatt ctccagcgga gtgtaaacgt tttgatgatg
     5881 aattaaaccc gcaagtattt tcatttctat aagtatgaac actatgaata ccagcaaggg
     5941 gatttttaat atatacaaat tatcactcct gttatttagt ttgtccggtt tctctgttgt
     6001 tgcacaaaca gggaattaca ataaatatat tcaggaatat caacaagcgt atagtaagaa
     6061 tcatgaagta gtaaaaggag atgacaaaaa gaatatacgc ttctttccgg cagatgaaag
     6121 ttttcgcatt caatcatcgt ttgaaagaat atatgaagcc ccgtggttca aaatggaaac
     6181 ttcaggtaaa gagaaaaaaa tatacagagt ttatggcatt ctccgtttca caataaaaga
     6241 tacaagcctt aaacttcata tttaccaatc acagcatttg atgtcggtaa aggagtatag
     6301 caatcattta ttcattccct ttacagacct ttccaatggt gaagaaagct atgaaaatgg
     6361 cagatacata gacctggtca ttgctgatgt agaaaacggg aaatatgtca ttgatttcaa
     6421 taaagcctat aacccatatt gcgcttatat taataatgtt tataactgtc ctgttcctcc
     6481 caaagaaaat aaccttaatg tggcaataaa ggctggtgaa atgaagtatg ggaagaaata
     6541 ttgagttatt tcagaaaata tagtcccctg tttaagcctg gattgaattt aaaacagatc
     6601 catctgttac ttaacaacac tcatatttaa gaacataatt agctgaatgt tctttttagt
     6661 ccttccaaaa acgatacagg ggaatatgca agttctcttt tagccttatc aataataaag
     6721 cctgtcctgg ctggtctttt ggcaggctgt gagaaacttt cagctgttac acgtttaata
     6781 agagatttgt ctaatcccag aaagtcggcg gtcttgcagg ccatctcata gggtgttaat
     6841 atgttaatcc cactaagatg ataaatacca cagactcttt tccccataat tgcaataatt
     6901 cctgctgcga gatcttcaac ataggtaggt gtccggacct ggtcatctac aacattatac
     6961 gcttcacctt tctccagttt ttctttaact attgtaagaa tattactgcg tccggccatt
     7021 ggttttccat aaaccagcac cgttctgact atggcccaat caagattata ccgctttact
     7081 gcttcttcag cttccagctt tgtctttcca taaaaattta ccgggttagg actgtcaact
     7141 tctgtataca tccccgcttc tccatcaaaa acaaaatcgg tagagataaa aatgaaaaaa
     7201 catttgtgtt ttgcagcatt tgaaagcatc gtaactgtac cttccgtatt tatcctcaaa
     7261 gcttcctttt ggtttagctc acattcatca ggtttactta cagcaccggc atgcactaca
     7321 atttctggtt tatgtgtatc aaaaatatca ttcacgacga atgggttggt aaaatccatt
     7381 tcggtatata caaatttttc tgaattactg aaagggagcc tgttattccc tttgcccgtc
     7441 gcaatcactt catatccttt ttgcaatagc agactaacaa gataatgacc caaaaaaccg
     7501 ttagctcccg ttataagtac tttcatactc caaatgtaga attatcaaat aaaaaatcct
     7561 gttctggctg aacaggattt tttatataaa tcagaaagtt acttaaggct tgtttaaagc
     7621 ttcaaccagc atggtcagtt ctccttcgct ttttagtttc aggctattgg cagcaacaaa
     7681 ctcattaatt tttaccgata aatccggtaa agcttctgca acatcttttt ttccaccttt
     7741 aatacgctta atggcaccac ctggaacata aatataaaac tgttcggttg tcacaatttc
     7801 acgtctgctg taatcgccta atgttgcagc atcctctact ttttggctca tgtattttaa
     7861 caggtgaacc ttaggtcctt ttgaaagcac ctggtaaata gttttttctg tttgtttatc
     7921 aatctcagga tagcctgaac caaattcaaa gtgcttcact gttccatcat tttgcacctg
     7981 atcataaaat acagatctga atgactccag tttcatatcc tggccatcct tttgcagcat
     8041 gatggcatta ttaaatatgt tgaatttaac agggatatta gagattgttt tcccactggg
     8101 aatctgtatg gttgctggat tatattcctg gctaaggaaa agtgaaccgc caacttcctg
     8161 cagtactggc aatctcatcg gtacgatctg caaatcttca aaaatcgcct gactattttc
     8221 tgtattggta tcagtagaat tttgtccaga caaatcgggc gcagtaaaag caaacaaaca
     8281 aattcccaaa aataacttta acgcataagt gattttcatt cctatttttt taagtgggca
     8341 aatatatcaa acgtataaat aaaaaatccg ccgtttataa atttaacatc cggcaatcga
     8401 tttattttca cacccattaa gactaaaatt gatatctggg tttgatatat aaaaaaatgg
     8461 ccatacaaaa cgtatggcca tcccttaaaa atttgttctt ttctaaaaag ctttttttcc
     8521 ggcttgtttt aaatttttaa aatcaggggt caatcccatg ttataaaaag tgaaagaata
     8581 aatatctgct gttgtttcga gtgccttttt gagattactg gcaccatggc cggaattgac
     8641 atctatcctg atcaaaacag gattttcgcc tttataatgc tcctgtaagg ttgcagcata
     8701 tttaaaagaa tgtgctggta ccaccctgtc atcatgatcg gcagtagtga taagagtagc
     8761 cggataattc agcccgcttt tgatattatg aatcggagag taagcaaaca ggtttttaaa
     8821 atcatccggg ttatcactgc taccgtattc ggctatccag ttccaaccta tcgtaaattt
     8881 atggaatcga agcatatcca taacacccac ttgtggtatc gccactttaa aaagttttgg
     8941 ttgttgatta atgacagccc ctacaagcaa tcctccgttt gagccaccct gaatagccag
     9001 gtaatcattt gatgtatact gtttctctac caggtattca ccggcagcaa taaaatcatc
     9061 aaaaacgttt tgctttttga aacgcattcc tgcttcatgc catttttctc catattcact
     9121 accgccccgg agattagcca aacagtaaat ccccccctgc tccagccatg ctatcctggc
     9181 cggtgaaaaa ctaggagttg tactgatatt aaacccacca tatccatata aaatggtagg
     9241 atttctcccg tttagctgca tccccttttt ataaacaata aacataggaa tcttcgttcc
     9301 gtctttactg ggataaaaaa cctgctgtgt ttcgtattct tcaggattaa attttacttc
     9361 cggctttcta aagacggtac tttgcccggt ggctatatca tatttataga ttgtgggtgg
     9421 aaaagtaaaa gaggtgaacg tataaaaagt aaatttatct tctctttcgc ctccaaaacc
     9481 acttacagtt cccaatgcag gcagtttcac ttccctttca aaatttccat tcaggtcata
     9541 ctggtaaaca cgactggtca catctttcat ataggtgata aatagtttat taccagtagc
     9601 tgtaaccgaa gacatatttt cctctttttc ttcaataact gttttccaat ttgcaggagc
     9661 aggattcaac ggatccacca atactatttt cctgtttttt gctccgtcat tggtttgcaa
     9721 aaggaattgg ccatttactt cttctacaaa actgtactga taatttccta cttctttaat
     9781 aacaggttta aactttttat cagcgctttt attatcaaaa taccagaggc tgttgccctc
     9841 ttttccttta cctctgtcac ttatattcaa aaaaacatat cgttcacttt tatctgtaaa
     9901 cacactatga aaacgttgag ggttttcttt atcctcataa gtcagtacat ccttatcctg
     9961 gctggttccc actttatgat aaaacacctg gtggttttca ttttttgtag aaagctcctt
    10021 acccgcttct ggtgctggat agcgactata ataaaagcca tccccctgcc aggaggcccc
    10081 ggaaaccttt acccaggcta atgaatcttt caagtatttt tttgttgtca gatccatgac
    10141 gaaaaatgtc cgccagtcac ttccgccggc agatttacct accactgcat acttaccatt
    10201 ttttgaaatt gaaaaagcag ccagactggt agtcccttct gccgaaaatt tattaggatc
    10261 caataccaat tccggcttac cagccaatcc tttttgcctg tataacacac tttggttttg
    10321 caggccatca ttcttcgaat aatagaaata ttcatttttc cgggaaggtg atgaatactt
    10381 ggggtaatta tttatatctt ctaaccggga ctgccattct ttccggaaag agatctgatc
    10441 aagataattg gttgtaacct tattctggtt agttacccat tcttttgtct cattactcca
    10501 atcatcttcc agccaacggt aaggatccgc cactttagtt ccatggtaat catctaccac
    10561 ttccccttta ctggaaaccg ggtactgaac ctgggctatt gcataaaacg gcaataacaa
    10621 caacagtgaa aatattcttt tcataattag caattttgca ggattaaaga tagaataata
    10681 tgcctgcaaa aattaccccc cttatctctt ttaaaaatta acctgaccag tggttttatg
    10741 ggctgacaag tgactactta ttttttataa ctgttggtgc cccttcactc agccaggttg
    10801 caaaaatctc agaaattttc cactcctttc cggcgtaaag tcttggacgg gcaatcatgt
    10861 agtctatgaa attttctata tctgccggtc cgcattgggt cattacatct aatactctct
    10921 tccctttata atcacccccc tttataaaca cattcattga ggggtcatta ttaataaaat
    10981 aatttcctgt aaattttata tccattatca ctgaatcaat gagcttttgt tcatcattcg
    11041 ctgtccattt agaaaacaag gtgtacctgt catagaatgg atcactgtaa acattttgaa
    11101 gccgtataaa atgaccagcc aattgtgcga tacggccata gtaaatattg gtcttctcca
    11161 tcatcgtata cagaagatct cctttctgca aaggctggcc gcttttgtta ttgcgtattg
    11221 aaaaatagta atactggcct tttataagat tacatttacc ataacccttt tccacggcat
    11281 catctttagc tgttctgtag accccgtaaa taattcccgg ttgcttatcc tttattatta
    11341 atccctcagg catttctaat tgtacaattt tggtggtatc atccaaatca accatgtagg
    11401 cagtaattgg gaaagcacgg agccactttg ttgagtcaga ttgagcaaaa gaatttgctg
    11461 aacttagcag taataaataa aaaagtagtt ttcgcataac aaatttttat aacacaactt
    11521 aatacggcca tatctacttt tttatcccgc ctgatgggta taattgttaa taaaagatga
    11581 attaatctga tatttgtgcc gttcaaaata ctaacaaatg tgtgttcgat acacatttgt
    11641 aacaaaagct tatttttgac ccgtttaaat tcaataatgg aaaagaaagt tacaaaaata
    11701 ggtgtgctta cttccggggg tgatgccccg ggtatgaatg ctgctatccg ggcagttgtt
    11761 cgtacaggaa tattctatgg gcttgaggtt tacggaatta tgcgtggtta ccagggtatg
    11821 atagaagatg atattatcaa aatggatggc cgctcagtgg caaatatcat tcaacgtggt
    11881 ggaacaattt tgaagacggc ccgttgtaaa gatttttttg aaacggaggg cagaaaaaag
    11941 gcctataaca atctgaaaaa aagaggcatt aatgggcttg taattatagg aggtgatggt
    12001 tcatttcgtg gtgctgtgaa gtttagccag gagtttgaca tcccctgtgt tggcttagcc
    12061 ggtaccattg acaaagatat ttcaggtact gattttacaa taggctttga tactgccgtc
    12121 aatacagccg ttgaagcaat tgataaagta agggacacca tggatgcaca tgaccgtatt
    12181 ttcattattg aagtaatggg aagagatgcc ggatacattg ctttgcatag tggcattgcg
    12241 acaggggctg agaatatttt gatccctgag cgaaaaactg atatgaagaa tattattact
    12301 tctttgcagg aaaaagaaag aagaaaaaaa ttggtcaacc tgattgtagt tgctgaaggt
    12361 gatgattttg gtggagccaa tgaggtacag aagatactcc aggaaagtct gccaaaagca
    12421 gaaatcagag tttgcatact ggggcatatt caacggggcg gctcacccag ttgcatggac
    12481 aggcttattg caagtcgtat gggatatcat gctgtggaat cgctgattga aggcaggcat
    12541 aatgtatttg ttggaataca aaacaataag atgaattaca ttccccttga aacagccgtg
    12601 aaaaacaaag gaaagatcag cgatgaatgg ctgaagattg ttaaaatatt agccagttaa
    12661 aagttcacat ctcttcactt gaaaaaagta tttgcttcgt atgacaaaaa atattgataa
    12721 atacctgcat aaagaattcg ataaagaggc cggaagacag catgcttata aaagaacaaa
    12781 aatagttgcc accgtaggac ctgcctgcga cacctatgaa aaattattag aacttgtaaa
    12841 ggccggtgtg aatgtatttc gattaaattt ttcacatggt gagcatgagg acaagaccaa
    12901 aatcataaat tttgtttacc agatcaataa aactgaacca tataatattg ctatcctggc
    12961 tgacctgcag gggcctaagt taagagtggg agaccttgaa ggaggggcac tcgaaataaa
    13021 tgatggcgac atcctgacat ttaccaatga aaaggtaatg ggtacgaaag aaaagatatt
    13081 tgtttcatat ccggatctcc attctgatgt aaaagtagga gaaaaaatac ttatcgatga
    13141 cggaaagctt gaagtgatag tcagggagat cacaaaagac aaagaagtaa aagtggaggt
    13201 tacattaggc ggaacattat atcccaaaaa aggggtcaac cttccggata caaaaatatc
    13261 attgccttcc ttaactgaaa aagatcttaa tgatctgaat tttattttaa aacacaggat
    13321 tgattgggta gcactttctt ttgtaagaaa tcccgaagac atcatcgacc tgaaacgaag
    13381 aattaaacag gccgggcata aagcaaaagt tattgccaaa attgaaatgc cacaggctat
    13441 cataaatctg cgggatatta ttgtagagag tgatggcatt atgattgccc gtggcgacct
    13501 gggcgtagaa ctcccgattg aagaagtacc acttattcaa aaagatatta ttaaaaaatg
    13561 cctgcatcgt gccaagcccg ttattgtagc cacacagatg atggaaagca tgatagacag
    13621 aaccaagcca aacagaagtg aaataactga cgttgccaat gctgtgctgg agggtgctga
    13681 tgcagtaatg ctgagcggag aaactgcaac cggcaaatac ccaaagctgg tggtagagac
    13741 aatgacaaaa ataatcctgg agattgaagc aaaagcttac gattataaca gggaagaaat
    13801 gttgcgtccc cagggacatt caccatcgtt cttaagtgat gcgatttgct acaatgcctg
    13861 taaattggcg gaagatgtaa atgctgatgc cattattggt atgacacaga gtggctatac
    13921 cgggtttatg cttagcagtt acagacccaa gtctttgcta tatattttca cgaaagaaag
    13981 atcactggta aatcaactga gtctaagttg gggtgtgagg gccttccatt atgatgaaga
    14041 agaaagcctg gatgacattt tttctgacca gattgatata cttaaagaaa gaagtttttt
    14101 aaaaaatggt gatgtggtag taagcaccgg aagcaccccg gtacatcttc acttaccaac
    14161 caatgttttg aaaatagcta aagttgaata acggaagtta actatagata ataccaactc
    14221 tcaaacaatt gtaaccagga aaggcaggtg atacaccagc ctttcctgat tccgggtaat
    14281 tacaattttt taatcataat ttttctgtac cagactgcat taccatgatc ctgcaggcct
    14341 attttacctt ttttataagt cccgaaatca ggcatttcaa caaacttact tcctgctact
    14401 aatttcttcc agttatcatc ccacaaagta gttgatacca cattttctcc attcaggaac
    14461 aggtcgagtt taccatttaa acatttcact tcagccaggt tccattcacc agccggtttt
    14521 actgtttcct tactgcagga aatcaggtcg tacaggtcac ctgccctgtg cttgataatt
    14581 tttgaatcag gatgaccatt attgtcaagt acctgcatct ccggggctgt ttcccagggc
    14641 catttgtact ttactgtatc ttcatgcaca tagaacatga taccgctgtt accatctttg
    14701 gctattttcc attccagttt caggtgaaaa ttttcaaaag cagagtcact cacaatatct
    14761 cctccatctt taatctgcca gttctctttt atggttgtat caagatacaa tgcaccatca
    14821 gccactttcc atgcactacc tacaggtttt ccgccatatt tatgccagcc tttggtactt
    14881 tctccatcaa acaataattg ccagccttca gtcttctgct gatctgtcag cacattcatc
    14941 cccaatcctt ctttagcagt tattgtggtg gaatctgtcg cttctccggt ggcatcattg
    15001 tcattgcaag ctattataga aagcaatacg atactacaaa caagaaaaaa tttcattaga
    15061 cttatttttt aagtaagaga atatattgta ccatcgcctc tgcttcttgc cgggaaatac
    15121 ccggatgcgg tgtcatcatg gctgaacccc acattccatt tccgcctttg atgatctttt
    15181 gtgcaagttc tgccacttct gcagctgatg cacctttata tctttttgca atctggcgat
    15241 agctcggccc ggtaaataca tcatcaactt tatgacaagt caaacagttg ttcctggcaa
    15301 ccaactccag tcctttttga tagacggggt cattagatgc acttatagca gtttcatcag
    15361 tgccgctttc attttttccc tctttgctat ttcccgcaca ggcaacgagt atcattaata
    15421 agaaaaaaga aataatattt tttttaacca ttgtagagcg ataattgatt ctatcataac
    15481 ctgaaccaaa aataaacttt agtctaatcc cagcaataaa cggtttgctt tttcatctgt
    15541 gccacttgat gcgaagtcat caaatgccct ttcagttacc cttataatat gccgttgaat
    15601 aaattccgct ccttcccttg ccccatcttc gggatgtttg atacaacact cccactccat
    15661 cactgcccat ccggaataat catattgtgt cagtttactg aagatagttt taaaatcaac
    15721 ctgaccgtca ccgggagaac gataacgacc tgccctgttg gcccagtttt gatatccgcc
    15781 aaaggttccc tgtttgcctg ttggattgaa ttctgcatcc tttacatgaa aggctttaat
    15841 tctttcatga taaaaatcga tatactgtat ataatccagt tgctgcaata caaaatgcga
    15901 aggatcgtac aacaaacagg cacgggggtg attgtttacc tctttcagga ataattcata
    15961 agttattcca tcaaacagat cttcaccggg atgtatttca taacaaacat ctacgccaca
    16021 ttcatcaaaa taatttaaaa tcggggtcca tcttttgccc agttccttga atccttcttc
    16081 caccaacccg gcaggacgct gtggccatgg atggaaagta tgccataata aagatccgct
    16141 aaaggttgga tgtgcattca gtcctaaatg ctgtgatgct ttagctccgt actttacctg
    16201 ctgaacagcc cattctgttc ttgctttggc attgccacga tattcttcgg gagcaaagcc
    16261 atcaaataat gcatcatatg caggatgtac agctaccagt tgtccctgca gatgtgttga
    16321 caattcggaa atctccaacc cgcaatcatt tacaataccc ctgatctcat ccgcataggt
    16381 tttactttca gcggcgagtt gcaggtcaat acatcgttta tcccaggtag gaatctgaac
    16441 cgctttatac cctattgctt ccgcccataa gcaaattgat ttcaggttag tgaatggtgc
    16501 tttatccccc ataaactggg caagaaatat tcccgggcct tttattgttg tcatcggata
    16561 aaataattta aaagtcagtt tcaattacac gataaagtcg atccactttt cttttgattt
    16621 tgccgaagca atcacctgct caataaatac cattcctctt atcccttcat ctgctcccgg
    16681 aaaatctagc cattcttctt tcggttctac tccctccatt tttgctttta cacacaatga
    16741 aaaattacgg taaagattgg caaatgcctc gagataacct tccgggtgac cggcaggggt
    16801 acgggtatta tgttgcgcaa aagaacttaa ataactattt cccgacctcc agatttctgc
    16861 cggtttatcc agccacttta ctattaatga attggcatct tcctgtttcc attccaaccc
    16921 tcctttttca ccatacaccc ttattctgat attattttct tcaccggctg ccacctgtgt
    16981 tgctatcaaa acaccgcttg ctccattatc atattctaat aatacagctc catcatcgtc
    17041 gagtttcctt ccctccacta caacattaat tgctgcgcag attttggtcg tttttaatcc
    17101 gctgacatac tctgccaggt taaatgcatg cgtaccaata tcccccattg aaccggcaat
    17161 tccactcctg gatggatctg tcctccaggc cgcctgcttc tgatttgctc cctcaagaaa
    17221 agtactcagc catccctgcg gatattcaac atatactttc cggatcactc cgaactttcc
    17281 actcaacacc tgttgcctgg cttcttttac catcgggtag ccagtgtatg tatgcgtaag
    17341 acaaaaatag ttgccgcttt tagctgcgat ttccttcagc tcttttgctt ctttcaaatc
    17401 aaaagtcatt ggtttgtcca gcaccacatg aaaaccattt tccaatgcca gttttgccgg
    17461 ctggaaatga agatgattgg gcgtaacgat actgacgaaa tccatctttt gatgttcagg
    17521 taatgcagct tctctacgga tcatctcttt ataggaacca tacactctat ccggggaaag
    17581 atataatgct tttcccattt ccgctgatct ctccggatca ctgctgaatg caccacaaac
    17641 cagttctatt tccccatcca tattggctgc aatacgatgg accgccccta taaaagcacc
    17701 cgggcctcca cctatcattc ccattcgtaa ttttcgtaat accatcgcaa acgtttgagg
    17761 ttaattctgt gtattaagtg ataatatagt atagcaaaat tgtgagcaat cgactgatat
    17821 gtatatttaa aaatagtaca tttaattttc caaaaccatt tttacaaaac taacgattca
    17881 acaaacaggc ctgcacatga aaacaacgat ccgcatccag ctatccacga tgatgttcct
    17941 gaatttcttt atttggggta tctggtttgt aacaatggga acctatctcg ggtcgaaact
    18001 aagtgctgac ggagtccaga ttggattggc ttatggaacc caatccctgg gcgcaattat
    18061 tgcacctttt attatagggc ttattgctga ccgatatttt gcagcacaaa aaatattagg
    18121 catcctgcac ctggctggcg cttccctgat gtattatctg tcaacagtaa aagattttac
    18181 atccttctac ccggttttat tggtatacat gatcatttat atgcctaccc ttgcgctggt
    18241 caatgctata tcatttaaac aaatgagtaa ccctgaaaag gagttttcct ttatacgggt
    18301 ttggggcaca ataggctgga tcattgctgg cttactcatt ggctggttag cattggagaa
    18361 aaagggatca ttacaaaata ctttcatgat tgctgcaatt gtctctggtg ccctgagtct
    18421 ttttagcttt gccttaccaa atactccgcc accgaaagct gggactaaag ccagttttgg
    18481 agaaattgtg ggtcttgatg cattggggct tttaaaggat aaaaacttcc tgatcttctt
    18541 cctggcctcc atgcttattt gcatcccgct ggctttctat taccaggaaa caaatatatt
    18601 cctcaacgaa gtgggtgttc agaatgctgc cgggaaaatg acaatggggc aaatgagtga
    18661 aacaatattc ctcttcctga tgcctttatt ctttttgagg cttggtgtta aaaaaatgct
    18721 gctgatagga atgctggcct gggttattcg ctatctctgt tttggttttg gcgatgcagg
    18781 gagtggggta atgctattat atgcaggaat aatccttcac ggtatttgtt atgatttctt
    18841 ttttgtgacc ggtcagatat acactgacca aagagctggt gacaaaatcc gttcttctgc
    18901 acagggaatg atcactttgg ctacatatgg cgtaggaatg ctgattggat tttggtttgc
    18961 aggctttatc gcagaatatt ataaaactgc tgacggccat gcatggaaaa atatctggat
    19021 ggtaccggca gcaattgcag gagtgatact agtattattc tttctgcttt ttaaagaccc
    19081 cggtaaaaaa gaaaaaatgg aatcataaaa ttaattttaa tgttattgta tgagccaaaa
    19141 aaaaacaaga agggaattat taaaacagct gacagcaggt tctctggcgg taactgcttt
    19201 accatccatg agctcatttg tttctaattc tgaatacgaa tattcactga aaggcaacat
    19261 caatcattcc gtttgcgcct ggacctataa ttttttacca ctagacgata tgtgcagttt
    19321 ggtaaaaaaa ctcggattta ctgctattga cctgttggct cccaaagact ggtctaccat
    19381 tcaaaagcag ggaatcacct gttcgatgtg ctacacggct ggtaagatca gtctgacaga
    19441 aggatggaat aaaaaggata accatgactg gctcatcaaa gattttactg aggctattcc
    19501 actcgtggct aaagcaggct ataaaaatct tatctgcttt agtggaaacc gcaatggcat
    19561 gaatgatgat accgggttga agaattgcgc agaaggactg aaacagatca tgttactggc
    19621 tgagcagaac aatgttatcg ttcagatgga acttttcaat agcaaaattg atcacaaaga
    19681 ttatatggca gataaaagtg catggggtgt tgaattatgc aggaaaatag gctcgcccaa
    19741 tttcaaattg ctctacgata tttaccacat gcagatcaat gaaggtgatg ttatccgtac
    19801 tatcagggat aatcaccagt actttggtca ttatcataca gccggggtac cgggcaggca
    19861 tgaaatagat gagtcacagg agttattcta tccggcaatc atgaaagcga ttgcagcaac
    19921 aggctacaaa ggttatgtgg cacaggaatt cataccgaca ggaaaagaaa aaaaagaaaa
    19981 aatagctgcc ttaaaaaagg ctattaagat atgtgatgtg taaaccatag aaagaagtaa
    20041 taaaaaaatc ggaaatacct tatggcagac aatctatatg acgctatagt aattggttcg
    20101 ggtatcagcg gaggctgggc agctaaagaa ctcactgaaa aaggtctcaa agtgatcatg
    20161 ctggaacgtg gacgaaatat tgaacatatc aaagattatg taagcgccac caaagaatcc
    20221 tgggaattcc cgcaccgcgg aggccgcacc cagcagatga tcaaggatta ccctgtttta
    20281 aaaagagatt acccgttaaa cgaaacagta cttgatctct ggtgcaagga ctctgaacat
    20341 ccttatacag aggtaaaacg tttcgattgg taccggggtt accatgtagg tggccgttcg
    20401 ttaacatggg gccgtcaaag ttatcgtttc aataaatggg actttgaagc aaatgcaaaa
    20461 gaaggaattg cagtggattg gccaattcgt tatgatgacc tggccccctg gtacagctat
    20521 gctgaaaaat ttgccgggat acaggggaac aaagatggtt tggaagtttt accggatggc
    20581 gattttatgc ctgccatgga gttgaactgt gtagaaaaag atataaagaa aagactggaa
    20641 acatattaca aaaacacaag acaccttata atcggaagaa gtgctaatat tacccagcct
    20701 catcacgaca gaaccaactg tcaataccga aacagatgct ggagaggctg cccatttgga
    20761 ggttatttca gtacacaatc tgccacctta ccggcagcaa tgaaaacggg caatcttact
    20821 cttcgcccat tttctatagt tacaaaaatt ttatacgata aggataaaaa gaaagcaacg
    20881 ggtgttgaaa tactggatgc tgagacaaat atgacatacg aatacaaagc caagattgtt
    20941 ttcgtttgtg catctgcttt gaattctaca tgggttctga tgaattctgc taccaatata
    21001 tggcctgacg ggctgggcag cagtagcgga gagcttggac ataatgtaat ggaccatcat
    21061 tttcgctgtg gcgccagtgg cactgtcgaa gggttcagcg atcaatatgt ctatggacga
    21121 agaccaactg gcatttatat cccccgtttc agaaatatct atactgataa gagagattat
    21181 atccggggtt ttggttacca gggcggtgct ggtcgtggcc gtggtgtaga agttgctgag
    21241 ttcactattg gcgctgacct gaaagatgca ttaagtattc ccggtgataa ttggggcatg
    21301 ggtattaccg cttttggcga aatgcttcca tatcatgaaa ataagatcgc attagacaag
    21361 gagaaaaaag ataaatgggg tctgccggta cttgcatttg atgtggaaat aaaggaaaat
    21421 gaaaagaaga tgcggataga tatgatgaat gatgccaaag aaatgctgga agcggccggt
    21481 gttaaaaaag tggagtcgta cgatcatggt tatgctgtag gaatgggtat tcatgaaatg
    21541 ggtacagccc gtatgggccg tgacccaaaa tcatctgtac tgaatggcaa taaccaggta
    21601 tgggatgctc cgaatgtatt tgtaaccgat ggcgcctgta tgacatccgc ttcttgtgta
    21661 aacccttcat tgacctatat ggcaatgact gcaagggccg ctaattttgc cgtcagtgaa
    21721 ttaaaaaatg gaaacattta aattctaacc tgatttttat atggaacgca gagaactatt
    21781 aaaattgatt gccttactta caggtggtgc agtggttggc ggagaagtat tccttgctgg
    21841 ttgtaaaaca gcaggaaagg cagatactgg ttttacaact agtactattg ccctgctgga
    21901 tgagattggc gagaccatta ttccggcaac agttactccc ggggctaaag cagcaaaggt
    21961 tggtgagttt atgaaagtga tggttacaga ctgctataca gaaaaacaac aggcagcctt
    22021 taccaatggc atcgcccaac ttgaagaagc ctgtaaaaaa gccactggca aatccttcat
    22081 ggaatgtatg ccggagcaac gtcatgactt cttagtaagt cttgagaagg aagccaaaga
    22141 atttaacaat aaaagaaatg aagaagacaa gccgaagcgg gaagcacata aagcggagaa
    22201 cgaaaaactg gcctttaagg atcagaaaga atttgaagga acacccagtc attattacac
    22261 catgatgaaa caactgacat tacttgggtt cttttcctcc aggaccggca tgacagaaac
    22321 ccttcgtcat ttgcccgttc ccggcaaata tgatggtgct tacccttata caaaaggtga
    22381 taaagcttgg gctgaataat tttgtaaccc ttataaaaag aagcaatgga caacagcaga
    22441 agaaaattca ttaagatcac aagcggtgtt accaccggat ttgcattaag cggtctttcg
    22501 ggtttatcct atttttcttc ctgtgaagaa acaggaaagc ttaaaaaatt tggcatccag
    22561 ttatatactt tacgggctga tatgcctaaa aatacaaagg gagttttgaa acaactggct
    22621 tcattcggat acagacaaat tgaaagttat gaggggccgc aaggtatgtt ctggggaatg
    22681 agtaatactg agttcaagaa atacatggac gatctcggga tgagtattgt ctcttcgcat
    22741 tgtgatatca caaaagattt tgaaaagaaa gcggctgagg ctgcagcaat aggtatgaga
    22801 tacctgatct gcccgtggaa aggcccgcaa aaatcattgg atgaattcaa aaaatttact
    22861 gacgagttca accagaaagg tgagatctgt aaaaagaacg gaatccgctt cgcttaccat
    22921 aaccatgatt atacttttaa gctcctggaa ggacaaatac cccaggatgt gctgatgacc
    22981 ggaactgatg cttcgctggt agactttgaa ctggatatgt attgggtagc tgcggcgggt
    23041 gaggacgtag aagcctggct aaaaaaatat cctaaccgtt tccgcctctg tcatgtaaaa
    23101 gacaggagta aaacacccgg aactgataac gggaaaaatt ctgtagatct tggtaccgga
    23161 agcatgaatt ggagcaaaat atttagtaca gccagggcaa acggcatgga atattttatt
    23221 gtagaacagg aggcctatcc gaatggcaca gcactggaag cggcaaaagt gaatgctgaa
    23281 tttatgaagg gattgaaaat ataaattgct agtataaaga actacgcaaa gccctgacct
    23341 cttcagggct tattgcatta ttggaataac tctctgccga atctgcaaat gccggatgaa
    23401 taaaatccaa cttacttttc tcctttcctc tcagggaaga atggaattct gtacagccgg
    23461 tttcttgtgc cagcatttta atgttttctt tccttacacc gcttcccggc attatgatga
    23521 tacggtcatt tgcttcccgg ttcaattcat ttatcaattt cacaccatcc ggtgctgcgg
    23581 gtttttgacc agaggtcagt attctttcac atcccgttac aattaattgt tctaatgctt
    23641 caaacggatt acggcaacgg tcaaatgccc ggtgaaaggt gactcccaac ggataagcag
    23701 cttccactaa ctgagccgtt cttttaacat caatacttcc atctgtattc aacaaaccga
    23761 tcaccacccc atcacaacct aattgcctgc acaacttcac ttcctgcaac atgatctcaa
    23821 actcttcaga ggtgtataaa aaatctcctc ccctgggccg tataatcggg tacaataaaa
    23881 cagggaaagc ttccctgcat ttttttatag tgccatagga aggagttgtt cctccttcag
    23941 ccaggttagc acatagctca attctgtctg ccccgccctg aactgctgat tgggttgtag
    24001 caaaatcaga agtagcaatc tcgattatat acctgacttc attcattgaa ccagctttta
    24061 gaagttacca gggtttcttt agtgccgctt ttaattgcat atttgaaccc cggatgtaat
    24121 gcaaaagcac ctgcctggcc ttttttattt aatgcaagga aagcaacctg aatatctttt
    24181 gccttagctt tcttgatctt tacaatccgc tcaattacct ttttacaggc cgcctccgga
    24241 gataaaccct gtcgcattaa ttccaccact gaatgtgaac cagccaccct tatcacatct
    24301 tctccctggc cggtggccgt acaagccccc acttcgttat caacaaataa accggcgcca
    24361 ataatgggag aatcaccgat acggccccgc attttaaaac cggcaccgct ggtagtgcaa
    24421 ctgcctccca tattaccatg agtatccatc gctaccatac cgatggtatc atgattccat
    24481 tcaccattgc taagtttcaa cggtgcaaat ggtccatggc cctgctcgcc tgcctgccgg
    24541 acaggcaggt tctctatatt aataaccgga ggcttatact cagaattctt taaccactct
    24601 tcatacgctg cctgtgcctg cttagaaagc ttttgctctt ccaatggaaa tccttcggtc
    24661 actgcaaact gttgtgcacc gcttccaact aacattacat gaggcgtttt ttccatcact
    24721 cttctggcaa ctgcaaccgg gtgttttatc ctttctaaaa aagcaacact gccacaattg
    24781 gaaaattcat ccatgataca tgcatccagt gtaacaaacc catctctgtc aggattggca
    24841 ccgagtccaa cacagcagtt ttgagaagcc tcagtcacca tgacgccttt ttccacagca
    24901 tccacggctc tgccaccctt aaataaaacc tcccacgctg ctttattcgc ttccagccct
    24961 gcatcccagg tagaaattac cacaggtttc cctttaatgg taaatggaag ctgattacct
    25021 gcagccattt tactgaataa aattcccagg gaaccaaatg ccgaagcatg aatgaacttt
    25081 ctcctgttga ccattgttta aatcgttagg tgttcgaaat taatggtttc gctaccaata
    25141 aaagacaaaa attgatttaa ctaaaccata agaaataata tttgatgttt aaacattgat
    25201 tctttacctt tacactttaa aacaccaaaa tgaaaataga aatcatttcc ggaagccccc
    25261 ggacaaacag tgtcacaaaa agagttgccc tccatttaaa gaactggatg ctacagcaaa
    25321 cgaatcacga aatcgatatg atagaaatga aggattggga tgttcccccg gttcaatccg
    25381 tttgggtgtc ggtggataaa gctcccgtgg aattgcaacc cctcgctaaa agaatctttg
    25441 aagcagatgc atttattctt gtcaccccag agtataacgg cagctattcg cctgccatga
    25501 agaacctgtt ggatcatttt cccaagcaat tacacaagcc atttggtata gtaactgctt
    25561 cacccggtgc aatgggtggt atgagggcat ctcagcaatt actattgctg gttcctgccc
    25621 tttttggtat tgcttcaccc aatatgctca tcacaccgtc tgtagataaa aaatttagtc
    25681 cggatgggga tctgctggat gaaagttttc aaaatgctgt tcatacattt attaccgaat
    25741 tcctgtggct gtctgaaaaa ctcgtaacag aaaaagtaat tgcataaata aaaggagcaa
    25801 atatgaaaac catacttcac aaagccaata caagaggtca tgctgatcat ggttggttga
    25861 attcttttca caccttcagt tttgccggct attatgatcc gaatagaact catttcggtg
    25921 tattaagagt attgaacgat gatactgttg ccggagccat gggctttgga gcccatccgc
    25981 atgataatat ggagatcatt tccatcccta ccagtggtga cctggaacac caggacagta
    26041 tgggtaacaa gcaggtaata aaacagggtg atgtacaggt aatgagtgcc ggaactggtg
    26101 ttacacattc tgaaaaaaat aagaatcagg ataaagaagt aaaattcttc cagatatggg
    26161 tattcccgaa caaaaagaat gtggtacccc gctacgacca gaagaatttt tcagctgctg
    26221 acaagcataa cagactattg acagtggttt caccgattgg ctctgcagac ggaggcgtac
    26281 aaattcacca ggatgcatgg ttcagcttag gtaatttaga taaagacttt agggtcagct
    26341 accagttgaa aaataagaac aacggtgttt atgcatttgt gattgaaggt gaagtaacta
    26401 ttaatggtga aaaactaagc agaagagacg gtcttggtat cactgaaaca gaccagttga
    26461 atatacaagc cgatagtgct gcagaattac tgctgatgga aatacctgtt taaaaaaaat
    26521 tatgaaaaac aaaagatcaa tcgaacatat cattgctcca ccacccccac atatggtagg
    26581 tgacgggttc cgggtgcata gtttctttcc cggtgcaaaa ttgatagata aaaaaagaat
    26641 gagtcctttc tttttgatgg actatggttc aaaaattgaa tttgcccctt ccgatgaacc
    26701 gagaggtgtg ggtgtgcatc cgcatgctgg ttttgagaca gtgactatcg cctatcatgg
    26761 aaaaatagcc catcacgaca gtgcaggaaa cagtggaata ataggtgaag gtgatgtgca
    26821 gtggatgaca gccggcgccg gtttgcttca taaggaatat catgaaaaag aattcagcaa
    26881 aaccgggggg cctttccaga tggtacaact ttgggtaaac cttccttccc aatacaaaaa
    26941 aacaaaaccc aaataccagg agattactaa tgcaatgatg ggtcgtcact acctgtctga
    27001 tgaaaaaagt gtcgttgaag tgattgccgg tgattacaac gggacaaaag gacctgcttc
    27061 cacttttact ccgatgcatg tttataatgc aaaactgaaa aaaggtgcaa gcactatcct
    27121 gaattttcct tctcactata ataccgggat gctgatcgtt gacggaaacg caatgatcaa
    27181 tgaccaggct gtaccaaaag atcattttgt attattcaaa catgatggag aaataataac
    27241 tatagaagca acagaagatg cagtgatact tgtgttatca ggcgaaccta tcaatgaacc
    27301 aattgccgct tatggcccct tcctgatgaa ttcctggccg gaagtagaac aggcaataca
    27361 agacgtgaat atgggaaagt ttggagtatt ggaagattaa aagcaaattc tatacaatga
    27421 atgttatgcc gggcctgacc cggcatcttt tttttcaata aatgcaatca aacttttcct
    27481 tgcattgttc tgtatccttt tcttcataaa cccagtccag ccaaacaagg ctcccttcca
    27541 gcccaatgcc tgtgcagccc attcactgag tttgaaggca tcactgtgtt ctattatctt
    27601 gccgtctttc aatttcatat aggctttgat atggttcacc acttttcttc cggttttgga
    27661 aaaagtgtat gtggccgtcc agttacaagt ggcgtattct tcatccagta attgaatatt
    27721 ggaaaatgta agtgaaaaat ccctggcatt cttacaaagc atttcccaca tactttttgc
    27781 ctcctctccc cgcagtaatc caaaagcagg gtcattgaat acaatatcat cactgtaaca
    27841 actattcata gtccgggagt ccaattgttg aaatgctgta taaaacctgc tgattattgc
    27901 tgtattcgtt tgcataaaat aaagataaag tgattgggga ttcagtaaat ttaaaccttc
    27961 aaaagcaacc ggcatgaaaa aaattataat cacaatccta ttatggtttt ccgtctttat
    28021 tgttattgcc caatccgatc cgcaggattc tgcatggatt aaggataact ataccaaact
    28081 ggaacaatat attcctatgc gggatgggat caaactcttt actgcaattt atatccccaa
    28141 tgataagaca gaaaagcatc cgatactggt aaccaggact ccttactcct gtgccccgta
    28201 tgggaaagag ttttcttccc gcctgtggga gcgtcactgg aaatattatg caagagaaaa
    28261 ttatatcatt gtggtgcagg atgtgcgggg ccgctggatg agtgaaggtg agtttgagga
    28321 tgtccgccct tttaataaaa ataaaacggg tttagaaaca gatgaagcaa gtgacactta
    28381 tgatgccatc gactggatgg taaaaaacct gccaggcaac aatggcaatg tgggtgtatt
    28441 tgggatctcc tatcctggtt tctattcaac tatggcagca gcatcgggcc acccggcctt
    28501 gaaagcggtt agtccacagg caccagtgac tgactggttc atgggtgatg attttcacca
    28561 caacggagct ttctttcaac tggatggttt tggtttttat tccggttttg gcaaaccccg
    28621 gccaaaaccc acaacaatcg gctcagccgg attcaatttt aaagtaagag ataattataa
    28681 attcttcctg gaagctgctt cattaaaaaa attggcagca ttaatgggtg atagtataca
    28741 attctggaaa aaaatgtatg cccatccgaa ttatgataca tggtggcagg ccagagatgc
    28801 ccggcgtgct atgtataatg taaaccctgc tatgctggtg gttggcggcc tgtttgatgc
    28861 tgaggattgt tatggcgcct ggaatttata taaggctata gaaaaacaaa atccgtctgc
    28921 caataataaa attgtaatgg gcccctggtc gcacgggcaa tggtccagtg gcgatggaag
    28981 ttttctgggc aatgtaaaat ttgggatgaa cacagctcat tggtaccaga acaatattga
    29041 gatccctttc tttaattatt atttaaaggg caaaggtgaa gtagaaaaaa ttcctgaagc
    29101 aacgatcttc ttcagtggag aaaatcaatg gaaaaaattg aatcaatggc cacctgcagc
    29161 tatgcaatca aaagatattt tcttaacaga gaatggaggt ttatcatgga cagcacctgc
    29221 agggaaagaa agtttctcag catataccag tgatccggct cacccggttc cctatacaga
    29281 agatgtccat catggacgaa cccgtgaata catgaccgat gaccagcgtt ttgcagcaag
    29341 aagaccagat gtgctgacct ttcaaaccga tgtgttaaca gaagatatta cactggccgg
    29401 agtagttacc gccaatctga aagtgagtat cagtggtaca gatgccgatt ttgttgtaaa
    29461 gatcatagat gtgtttccag atgatttcat atatcccggg gataaaccac aggctggccg
    29521 taccgctggt ggcagctatc caatgggtgg ttaccaaatg atggtgcgtg gagaaataat
    29581 gagaggaaaa tacagaaaca gctttgaaac ccccactcct ttcaaacccg gtaaagtgga
    29641 aacagtaaga tttgatctgc cggatgtagc ccatacattt aaaaaaggac atcgcctgat
    29701 gatacagata caaagcagct ggtttccatt ggctgaccgt aacccccagc agttcatgaa
    29761 tatctatgaa gccaatgaaa ccgattttaa aaaagctgtg atccgtattt ttcatgatgc
    29821 aaaaaatcag tcttcgattg ttttacccgt aatgaataaa taataccata tgcgattcct
    29881 gatctgcttt ttgtttgccc tttctgcatc aaccgtaact attgcgcagc agaaacaact
    29941 tgctcttatc ccttaccctg tagaagtaac acagggaaca gggtatttca cgattaaaaa
    30001 tacggttagc gtttcttcct ctctgcctgg ccacgaatgg aaatatttgt tttcctattt
    30061 caaagatgaa atgaaaaagc aatttggaat agcagtgaag gaagtgacaa agggccagcc
    30121 tgctgacatc agttttaata tgcggagaat gcctacctcc ggaaaagctg cttaccagtt
    30181 agaggtaact aaaaatggcg ttattatcaa cagtaatttt gctgcacctg cctttcacgc
    30241 cattcaaaca cttttccaat tatttcctgt tgataaaaaa gtaccagggc agatacctgt
    30301 tgtaaaaata ttcgatcatg cccgttttga ataccggggt atgcacctgg acgtaagccg
    30361 tcatttcttt ccggttgact ttataaaaaa atatattgac ttcctcgctt accataagct
    30421 caatacgttt cactggcacc tgacagatga ccagggctgg cgtatcgaaa tcaaaaaata
    30481 tcccggttta actgagattg gtggctggag aaacggaact attatcggcc gatatcccgg
    30541 tactggcaat gataatatca gctatggtgg ttattatacc cgggaagaaa taaaagaagt
    30601 ggtgcaatat gccaaagaac gcttcatcga agtaatacct gaaatagaaa tgccgggtca
    30661 ttcatctgca gctattgcag cttacccatg gctgagttgc ttcccggaaa agcctacgtt
    30721 gatcccggcc aatatgatct cgcaaaaaag tatagaagaa caaaaaaagg gaagggtcaa
    30781 actagtacag gaaacctggg gagtgtttga tgatgtattc tgcgcaggga aagactccac
    30841 ttttattttt ttgcaaaatg tggttgatga agtaattgaa ttatttccct ccaaatattt
    30901 tcatatcggc ggagatgaat gtcccaaagc tcattggaag aaatgtgtgc actgccagaa
    30961 acgaatgaaa gaaaacaacc ttaaagacga acatgagcta cagagctatt ttgttcagcg
    31021 tatggaaaaa cacctgaata gcaggggaaa aatattaatt ggctgggatg agatactgga
    31081 aggtggttta gcgccaaaag ccgtagtaat gagctggaga ggagaagctg gtggtattga
    31141 agccgctaaa caaaaacaca atgtgataat gacaccgggc aatcctgttt attttgatca
    31201 tacgcaaagt gaaaatgagg acagtgttac cattggcggg tacaacccca ttgaaaaagt
    31261 atatgcgtat gaaccaatac caaaagaact gaacgaagaa gaaggaaaat atgtactcgg
    31321 tgcacaggcc aatctgtgga cagagtatat agacaatata aaaaaagtgg agtacatgat
    31381 cttcccccgt atggcggcac tgagtgaaat actgtggagc ccgaaggaaa aaaagaattg
    31441 ggacagtttt gaaaaaagat tgctaacgca attcaatcgt tatgaaaaat gggatgcgaa
    31501 ttacagtaaa gcatattttg atttaaagtc ttacgtttta cctgctacga accattccgg
    31561 cataatctgg aaggttgaaa gcaaattaaa aaatgatagt atattcttta aagaagcaaa
    31621 gggtcttccc aaaccgatta agtatactga gcctgtttta ataagtgaaa caagtaataa
    31681 acatgtattt caaatattta atggcaataa gacttcttcc ataactcagc agttcaatat
    31741 caacaaagcc accggcaaaa aaataacgct tactactcca ccctccaaaa cttatcccgg
    31801 agatggagca tttacgttgg tgaatggggt gcagaatgaa aaaggaacgg caaagagtaa
    31861 agaatttctt ggattcagcg gaactgattg cgaagccatc attgattttg gaaaagaaga
    31921 atccatcaac tctataaaaa ttcatgtact tgaccaacaa agcagttgga tttatccgcc
    31981 tgcatggatg aaagtatatt acagcgctga tggaattaat tttacagaag ccctcaatgc
    32041 agaagctggc aaagaggtac ccggagaaat aataaaagat attgaatgga gcttttatcc
    32101 agtttcaaaa acaaggtacc tgaaagtatt ccttaaaaat tttggaacaa tccctgaagg
    32161 caaacccggt gcaggcaata aagcctggtt atttgtggat gaaattgaag taaattaact
    32221 gcataaaccc gcaaataacc gcataaacct tcatttcaaa tatctatctt tacttttcaa
    32281 accaacgcaa tgtcaaaaaa agcagcctcc accaactttg ctgatagttt tgaaaaaatc
    32341 cccgttaata tttttcctga ccaaaaagaa gggtccgttt ttgtagccaa ccaaattgct
    32401 gcattcatcc gggaaaaaca acaaaaaaaa gaaaaatgtg tgctgggcct ggccaccggt
    32461 tctacgccta aaacacttta tactgaattg gtccgtcttc atcgtgaaga gaaactgagt
    32521 tttaaaaatg taatcacatt caacctggat gaatattacc cgattgataa taatgccctg
    32581 cagagttaca accgtttcat gaaaacacat ttgttcgatc atgttgacat taacccaaaa
    32641 aatattcata tccccaatgg tgagataaaa aaggaaaaca ttaaagagca ttgcaagcaa
    32701 tatgagaaaa tgattctcga tgcaggtggc attgatttac aaattctcgg aattggtaac
    32761 aatggtcata ttggtttcaa tgaacccggc tccggtattt attccaaaac aagattgata
    32821 acgctggata attccacccg gatagctaat gcctatgagt ttgccaatat ttctgaagtc
    32881 ccccggttgg cgatcacaat gggtatcagt acgattctga aatcaaaaaa agtgatcctg
    32941 atggcttggg gaagcgccaa agcacctgtt atcaaaaaag cagtggaaga agacgacagc
    33001 gaacaggttc cggcatcttt actgcagaac catgatgatg taacatttgt tgtcgatgat
    33061 tctgcagcag cagaactgac aagaaataaa tcaccctggc tcaccggtga ttgcgaatgg
    33121 acaccaaaaa ttatcaagaa agcggtggtc aatatggcgt tgaaactgaa gaaacccgta
    33181 ttgagtttaa ccaacagtga ttataacgaa tatggattaa gtgatttgct ggttgaaaag
    33241 ggtgatgcgt atgaaataaa cctgcaggtg tattatatgc tccgtgattc catcaccggt
    33301 tggccgggtg gaaaaccaaa tgcatatatt ccggcacacc cggaacgttc gtcaccatac
    33361 cccaaaaaag tgatcatctt ctcccctcac ccggatgatg acatcatcag catgggtgga
    33421 acttttcagc gattacatga ccagggacat gaagtacatg taggttatca aaccagcggc
    33481 aatattgcag taacggatga gtttgtgacc aggttcctcg atttcgctgt aggctttgaa
    33541 gaaattgcag gtattgatac cagtaagtct ggtaagatat tagacaatgc aagaaaattt
    33601 ttagcccgga aaaaatcaaa ccaggtagat actcctacta tccggtctat aaaaggattg
    33661 ataaggcagg gagaagcaaa agcaacctgc cgttacgtag gtattaaaga tgagaatatt
    33721 catttccaga atcttccttt ctatgagaca ggtaccattg agaaaaaacc catgagtgaa
    33781 aaagatgtga aaataaccat cgagttattg cgcaaaataa aaccacagca ggtttattgt
    33841 gccggcgatt ttgctgaccc gcatggtaca catatcgttt gtttcaacgt ggtgctggaa
    33901 gccttaaaaa gaataaagaa cgccggcgat gaatggatca gtgattgctg gttatggctc
    33961 tataaaggtg cctggcagga atggaatatt gaagaaatag aaatggctat cccgatgagt
    34021 cctgaccagg ttttaaaaaa acgtttcggc atttttattc accagtcaca gaaagatatg
    34081 gttcccttcc agggcagcga ttccagagag ttctggcaac gggcagagga aagaaatgca
    34141 gctaccgcga atctttatgc agatttggga ttgacacatt atgccgctat ggaagccttt
    34201 gtcagatggc attattaacg atactatttt gaggatttcc cttaatttta atgataataa
    34261 aaaatagcaa ctatgtccat ttttaaatca agtaacccta cccttcagga aaaaacttac
    34321 cagggaacaa tcctggaagg aatcagtacc ggtgaagaaa tgactcttaa aggcactatg
    34381 aacaaatttg gagtattggt aatgctgatg atcggctcca ccctttttgc atggagccag
    34441 ttttataaag gcagtgatcc caaaatattt atgatggttg gtgtttttgg tggtttggca
    34501 ttagcgctag tgatggcttt taaaatgaaa tggtcaacat ttttggctcc ggcttatgca
    34561 atattggaag gtttatttgt tggttctatc tcggcaatgt atgattatat gtacccggga
    34621 cttccgctgc aggcagtagc gttaacacta ttggtaacct taatcatgtt cctgatctat
    34681 cgttaccgca ttataaaagt aacccagaag ttgagaacgg tagtggtagt ggccacagcg
    34741 gcgattgcag tattctatct tgttcaatgg cttacttatt cattttctag tactgcggtg
    34801 gcctattctt ttactaattc atcttccccc atcagtattg gtttttccat cctggtttca
    34861 ggtcttgctg ctttcaacct gttactgaat tttgatacag tagaaaaagg ggttgaaatg
    34921 agagccccga aatatatgga atggtacagt gcttttggac tgttggtaac tgtagtctgg
    34981 ttgtaccttg aattattaag attgctgtca aaattcaaca gaaattaatt tatacgcatc
    35041 atcttaagac cctgtctgcc agcagccgac agggttttta tttttcaact ccctggtaca
    35101 tttttgatgt aaattttttg ccctatattt gcaacccgct ggccccgtag ctcaattgaa
    35161 tagagcattt gactacggat caaaaggttt caggtttgaa tcctgacggg gtcaccacct
    35221 aagaaaagga ctacactatt tgtagtcctt tttacttttc cgattgaaat aactccccaa
    35281 aaaaaataga aaatattaat acccaatggg atcaaatcct ggtatactat cctgaaatac
    35341 ttaaattttt tatttttcct gcccctgtaa atcgggtaca gttttactat tttcatttgt
    35401 atttattccg gtttcatccc cttcaataac agccatccca tctaaaaaat acatatcaat
    35461 atccccgata ttcttcgcat taattttacc ccgatagctg caggaatatt tatctttaat
    35521 cagttcaaat acggaggaag aaatatttac tttacccggc ataccgttac tttcaagacg
    35581 gctggctatg tttactgtac ttccccaaat atcataggca tattttttct ttcctactac
    35641 acctgcaatg acaggcccca catgaacacc aattcttaaa tcccatgact ccaacccttc
    35701 caggtttctt ttttgattat actggcggat aaaatgacat atttccaaac tggcttttac
    35761 catattctct acatgatgct catctgctgt ggggataccg cctgcacaca tatacgaatc
    35821 cccgattgtt tttatttttt cgaggccata ttttccaatg atatcatcaa atgctacaaa
    35881 gcaggtatta agctcctgta taagttcctc cggagacatt ttatcagcta tagctgtaaa
    35941 ccctttaaaa tcggtgaaca ttacagaaac aaattcataa ttccgcggga tagcatgccc
    36001 atatacctgc agttcatttg ctacttcaga cggtagaata tttaataaca aactttctat
    36061 ttcacccttc tggcggtcaa gaattttatg tgtcctgact ttaatccggt agttcctgaa
    36121 aatgagtaat gcaatcagta atacaagtat caacccgaca ataagtacct tttttgtgaa
    36181 ctgctgtctc ttcaacttaa tatccgtgag cgccatgtcc ttagtaagta aatttatctc
    36241 gccctgtttc ttttgaagat cgaagtcaaa ctgaagtgta cctaattttt tatccgtatc
    36301 gatattatac aaagtgtcct taatactgga aaacaatgtc tggtatttaa aggcattgct
    36361 gtaatccttt gtcttcgaat acgcaagagc catttcctgg tacaagtcct tgatctcatg
    36421 atttgcatgg atctccagag ccgggagttc agctttcctg aaatattgaa gtgcagttgg
    36481 gtaatctcct ttttttacat acacattagc aagccccatc aaagattgaa cgattgatat
    36541 tttgttattt aatttctcag caagggctaa tgctttctgg tgattttgta aggcaagttc
    36601 ataattctcc tctttacggt aaatttttcc cagtccatta taagcgttaa ggcttccctc
    36661 cgagtcacca taggctttta atgatttatt gaaataaaat aaggcctgtt catcattatc
    36721 cttttcaaaa tatattgtcc cgatatttac acaaatagta ccggattcct ccatttttcc
    36781 caattcttca ctaatgctaa gggcctttaa ataatattca agagctttat catacgtggc
    36841 gggctttata ttgtatacac ctccaacatt attcaaagcg ataagaatcc tgaatttatc
    36901 accagccaat tcagaaaatt tcaaagattg caagtaattt tcaagggctt tcgcatcatc
    36961 accccggtcg taataaacta caccaatatt gctgtataga tttgcaattc cgctattgtc
    37021 ccctattatt ttaaaaactt ccaatgattg agtataataa tccaatgcct ccagatgttt
    37081 gccctggaaa taataaacaa taccaatatt ctttaatgcc agtgctgtcc ccttttggaa
    37141 atctatcctg tccgcaaggg cttttgcctc attggcaaga cttatcgcct tatcaggatc
    37201 attggtaaaa ttctctttgc tttgttgcag cagggagttt acccgggtag tatcaccagg
    37261 tatatccgtt gtagcaggct gagaaaatat caacagcgaa ataaaaaaaa gaggaaagca
    37321 gaaaacaaat ttcttccaaa ttatactaag ctgtaatacc ataaactgca tatcttttat
    37381 tttaatttaa gttacgcttc actctttaat ttatcccttt acaaatcgta atgtctgata
    37441 accgttttcg cctcttaccc tgatcaggta aacaccattt aataatcctg acacatccag
    37501 ctcgaagcta tgctctgaaa ttcttcttat aacttttaca ggataatatc tcccgtaaac
    37561 atcgtataag aatataccct tttcgctttg tacactttcc gctgttataa ttattaccct
    37621 gttcgtagca ggatttggat agagggaagt ttttgctgtt gttggcggct cttcataatt
    37681 actttcatca gtagcacccc tggctgctgg tgcataatta attgcacact ttacagaaga
    37741 ggaactggca gtgacagatt ctgtctttaa ataattcccc tcatacgttt ttaactgcca
    37801 ttttaatgta actccatcaa aaggaacatt gaaacgggtg gtgccgttac caggtaagaa
    37861 aacaactggt tgctgagagc catcaaatga acctgctgat gaaagactat tattcactcc
    37921 tatcggtaca taaacaggtg tactatttgt gttcttggca tagaaacgag ccacatacct
    37981 gcgattagca ggcatatagg aggcccctct gtcttcaaca cattccaggt atgtatctac
    38041 atcatctgct cccgaacctt tgggatttat atacaaataa ttattcgtat aagtgattgt
    38101 atagtttatt gaattagcaa atggtttgag taacggtata atagtataca ctcctgcact
    38161 tcctgtacag gaagggctta atgagtacga tactgttgga ttatctccat ttttcaatcc
    38221 tgtaatcgtt gatgtaaaag taggtaatgg actaccctta ttaatccatt tcaaatcagc
    38281 tttcacctgt agcggagctt tagtgataac aaaagttgct attggtacta ctgtggataa
    38341 acttaaactg taattagacc ctgcagacag gttacctaaa gtataagcat aagaacctgc
    38401 attgttcccg cttacacggc taagggctcc ggtaaagtca aaggctgaca ggccggcatt
    38461 atttgtgaat gtaaataccg ggtcagtact ggtaccataa actttacttt gcccgggaac
    38521 aggagtaatc gtaacagatt taatagtgat ggcaaaggtt ggaaccggcg ctactgatga
    38581 aagcagcaag ctataatttg taccggcact caaatttcct aatgtataat cataagtacc
    38641 aacattatt
//
LOCUS       DAORTI010000017        35819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_739_length_35819_cov_6.293507, whole genome shotgun sequence.
ACCESSION   DAORTI010000017 DAORTI010000000
VERSION     DAORTI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 35819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_35819_cov_6.293507"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLO70_04125"
     CDS             complement(<1..454)
                     /locus_tag="PLO70_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQZ73673.1"
                     /translation="MSTFIDQTGRTITLEKTPARIISLVPSQTELLFYLGLDKEVIGI
                     TRFCVHPEQWFRTKTRIGGTKQLKTDLIHQLHPDLIIANKEENIKEQVEELATRYPVW
                     VSDINNLKDAYEMIHSIANMVGKTVEGTELNAKIQFEFEQLKTIQNKIR"
     gene            complement(954..2312)
                     /locus_tag="PLO70_04130"
     CDS             complement(954..2312)
                     /locus_tag="PLO70_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQZ73674.1"
                     /translation="MDIKNNILETIGNTPLIRLNKITRDLPCTVLAKVDYFNPGNSIK
                     DRMALKMVEVAEKEGKLKPGGTIIEGTSGNTGMGLALAAVVKGYKCIFVTTEKQSKEK
                     ADILKAVGAEVIVCPTNVLPEDPRSYYSVAARLAKEVPNSFHMNQYDNLANRLAHYET
                     TGPEIWKQTDGKITHLVCTAGTGGTITGTAQYLKEKNPNIQIWAIDVYGSLLTKYFRT
                     GEIDMNEVHPYISEGFGEDFVPKNYDMSVIDYFEQVTDKDGAVMARRLAKEEGLFCGY
                     SAGSCIQGLLQLKDRLKKEDMVVCIFHDHGSRYVGKIYNDQWMIERGFLDVKTFRDVV
                     SSRGTKRLITVEPGQTVLEAVELMKKYDIEQIPVMNENGSVGAISEGGLFQKIFDNPE
                     IKNSTVQEVLEPSFPIVAFDTPVEKLSHLINKENGAVLAKDEAGNYHIVTKYDVIQAL
                     GN"
     gene            complement(2359..3015)
                     /locus_tag="PLO70_04135"
     CDS             complement(2359..3015)
                     /locus_tag="PLO70_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73675.1"
                     /translation="MKQITTLFAGVIFSFVLMAQQTTSVTAGIGCGDISIGMTEIQVK
                     AILGDSMEEKTFKEEMTAFWHHNRTVAIDSITQFVLGFDKCLVLDDDLNRKWPIFKIF
                     LLKGKVNCMIVTSYLPDNTLAEKVLINENIRFEDDAEKCKKYFGDQFIKTPYPGYDEY
                     LYYNKGIHLLFKEGVLKTFKLFKANPGFPALIAARAKLLKTQFLAIDKEKGGGFNWPD
                     "
     gene            complement(3130..3639)
                     /locus_tag="PLO70_04140"
     CDS             complement(3130..3639)
                     /locus_tag="PLO70_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73676.1"
                     /translation="MNTQRNIEERLWEFIDGHSSLEEKTVIERLLQTDTEWKAKYSEL
                     LEVNEMLQSSELEAPSMRFTKNVMEEIAKMHIAPATKSYINKRIIWGIGLFFMTMLVA
                     ILVYGFSQMDWSTGESSAITDKISKIDYTKFFNNTWVNAFMMINVLLGLVLLDNYFSN
                     KRKQFRKEA"
     gene            complement(3645..4226)
                     /locus_tag="PLO70_04145"
     CDS             complement(3645..4226)
                     /locus_tag="PLO70_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ73677.1"
                     /translation="MSTGLNDNELISKVLSGDHQAYAGLVNRYQNYVFTLTLRMVKNR
                     EDAEEVAQDVFIKAFKYLADFRGASKFSTWLYTIVNNTCISFLRKKKLEIHSLDNEKV
                     FEVADSMDSGFRANLVEQKSRQSMVHNAIGLMGPDDAEIITLFYKSEQSLEEIAQILG
                     LEVNTAKVRLHRARTRLKEKMETHFAHEVKDLN"
     gene            4468..4848
                     /locus_tag="PLO70_04150"
     CDS             4468..4848
                     /locus_tag="PLO70_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73678.1"
                     /translation="MDAGEVLIPITMFAGGFAMIFGIYYLKTRQNLAMIEKGMNPKEF
                     ANRPAPYKNLKWALLLIGAGTGLFLAYLLDNYMLPVEINKFGNHGDRNVPIYFALIAI
                     GGGLGLFGSYRIEKKWWDDNKDKQ"
     gene            complement(4897..6489)
                     /locus_tag="PLO70_04155"
     CDS             complement(4897..6489)
                     /locus_tag="PLO70_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQZ73679.1"
                     /translation="MGNKKLQVWLPLIFSLVLIGGMFLGFKLSDQSGSKRGFFSSNSR
                     STLQEALDLIKMRYVDSVQIDTLEGRAIQEMMNELDPHSVYLPPVELKEANEDLAGNF
                     DGIGVEFNIFSDTVNVVYVFPDGPSDKAGLVIGDKITRVNDSSLTRKGVSINEIKALI
                     RGPKGSKAILQVIRGAKQQSITVTRGKIPVPSIEASYMIDKNTGYIKLTKFSESSYEE
                     FMQAMEALQKQGMLSLVFDLRGNGGGFMNEAVDMADEFLEGDKLIVYTEGVNSKKREY
                     RCKRPGLFEKGKLVLLVDELSASASEVLAGALQDWCRATIIGRRTFGKGLVQEQYQLR
                     DGSAMRLTVARYYTPLGRSIQRPYEKGKKVYMDELWERFNSGEMVSADSMKNHNGFRF
                     TTACSDTLYGGGGITPSIFVPIDTSVSQQKANRLTFAANINSFVYNYYLQNKIQVDKY
                     NTTADFITGFNNTEGIWNDLVKFTATDSVNFKSVAGKEKELVQQRLKALLARYKWRNA
                     GFYQVLNSNDAMVKKAMETIAK"
     gene            6577..7419
                     /locus_tag="PLO70_04160"
     CDS             6577..7419
                     /locus_tag="PLO70_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HQZ73680.1"
                     /translation="MPVVKLIADSGATKAEWCLLQNGRKKTIITQGISPYFLNTEQIT
                     DLLLKELKPKLKNTNIDEVYYYGTGCANPVNAKSVKKAISKVFQHTKVEVTHDLMGAA
                     RALCGRKKGVACILGTGSNSCYYDGKKIVKNSPGLGYVLGDEGSGAYLGKKVIQYYLY
                     GTFDDELRGRFDLTYTTNASEILENVYKKPLPNRYLAGFAKFLAENRGHYMIENIIED
                     GLNDFFFNHLCKYRETWTLPIHFAGSVAFGFKDVLQQLCNSYEFELGRVMKNPMEGLI
                     EYHS"
     gene            7431..8246
                     /gene="murQ"
                     /locus_tag="PLO70_04165"
     CDS             7431..8246
                     /gene="murQ"
                     /locus_tag="PLO70_04165"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQZ73681.1"
                     /translation="MAFKKITEQSSNYRHLEKMTVGELLTNINNEDKTVPEAVERAMP
                     QIEKLAEIASDKMLMGGRLFYIGAGTSGRLGIVDASECPPTYGVPYGLVIGIIAGGEK
                     AITTAVEFAEDDREQGWMDLLEHKVSDKDVVIGIAASGTTPYVIGALDECRKRGIVTG
                     SISCNPDSPVSAAADFPIEVVVGPEFVTGSTRMKSGTAQKLVLNMISTTVMIQLGRVE
                     DNKMVNMQLTNDKLVDRGTKMLMERVKIADYEKAKELLLKYGSVKKAADSFVG"
     gene            8474..10918
                     /gene="thrA"
                     /locus_tag="PLO70_04170"
     CDS             8474..10918
                     /gene="thrA"
                     /locus_tag="PLO70_04170"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HQZ73682.1"
                     /translation="MQVLKFGGTSVANAENINKVVAIVKESVKKEKTIIVVSALGGIT
                     DLLLGAAAIAAEGNEAYKEKLSQVEQRHLDTVKQLIPVAQQSQLLSLVKKSCNEIEDI
                     CNGIFLLRELTPRSKDRIGSYGEWISSQIIAAKFIAEGLDAVWKDSRELILTNSTFTA
                     AEVDFPITNKRTGEFFAAASSTLFILPGFIAADKNGITTTLGRGGSDFTAAIIASAVN
                     ASVLEIWTDVSGMMTADPRLTSNARIIPHISYQEAMELSHFGAKVIYPPTIQPVMGKR
                     IPVWVKNTFAPADAGTLIESVATKDGDIVRGISSINNIALISLEGSGMIGIPGFSKRL
                     FEALSSERINVILITQSSSEHSICVGIDALSAAKAKQAVDSAFANEIALEKVEPLQIE
                     SALSIVALVGENMKSHPGISGRMFSAMGRNGVNVRAIAQGSSEKNISAVISTNDVRKA
                     INVLHEEFFETTYKQVNLFITGTGNVGAKLLGQLQKQLEFLQEQLHLQVRVVGLSNSR
                     KMLIDEEGIDLKNWKEKLEAGETASLQKLTDEIISRNLRNSVFVDITANDKVAGVYGL
                     LLQKSISVVACNKVAASSAYSNYKKLKDLAREFNSQFLFETNVGAGLPIIATLNDLVH
                     SGDRVHKIQAVLSGTLNFVFNNYDGSRKFAEVVKQAQEEGYTEPDPRLDLGGTDVMRK
                     IMILAREAGQEIEMDDIVNNSFMPASCMQGSVDDFYAAMLNEEAHFKKLAEDANIAGC
                     KLKFVASYDNGKASVGLQHIDPKHDLYHLYGKDNVVLFYTDRYKEQPLVVKGAGAGAE
                     VTASGVFADIIRASKV"
     gene            10920..11846
                     /locus_tag="PLO70_04175"
     CDS             10920..11846
                     /locus_tag="PLO70_04175"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQZ73683.1"
                     /translation="MKKVKVHSPGTVANIVCGFDILGLALSEPYDVMEVMLLDRPEVI
                     IHNRDIFNLPTEAEKNVAGVVLLSVMERMNNTIGFEVTVEKHIKPGSGIGSSAASAAG
                     AAIAANYLLGNIFSSDEVVQFAMNGEKLASGVKHADNIAPCILGGISLIRSIHPLDIV
                     SIPAPDLFVTVVHPQIEVRTSDARQILKQQVLLKDAIKQWGNIAGLVTGFMKNDYDLI
                     GRSLEDVIIEPVRSILIPGFDELKVKCKEAGALGGGISGSGPSVFMLSKDESTAKAVG
                     HVMSQVYEQIGIDYNIYVTTINRKGVEVVSSE"
     gene            11891..13186
                     /gene="thrC"
                     /locus_tag="PLO70_04180"
     CDS             11891..13186
                     /gene="thrC"
                     /locus_tag="PLO70_04180"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQZ73684.1"
                     /translation="MKYYSTNKIAGSVDFKEATIRGQAPDKGLYFPEIIPFVDKDMIS
                     NIDSYTNEEIAFRIIAPYVGNGIPEDRLWEIVKETVNFEIPLVKVNDTISSLELFHGP
                     TLAFKDVGARFMSRCLGYFVQGDSRKVTVLVATSGDTGGAVANGFYNVEGVEVVILYP
                     SGKVSPVQEKQLTTPGKNIHALEVQGTFDDCQQMVKQAFADKELTDKLFLTSANSINV
                     ARWLPQQFYYFYAFKQWEQKNNPPVISVPSGNFGNICAGILAMQSGLPVKHFIAACNV
                     NDIVSEYLQTQELQPKEAVATLSNAMDVGNPSNFVRILEIFKHQFPLLKNKLSAYTIT
                     DAETVSTIREVYEKYNYILDPHGAVGYLSLHRYLSAHPGQTGLFLETAHPVKFPEAVE
                     SCIGKCIEMPAAVQSLMDKEKQSELIKADYQQFRDYLLK"
     gene            13255..13821
                     /locus_tag="PLO70_04185"
     CDS             13255..13821
                     /locus_tag="PLO70_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73685.1"
                     /translation="MHNLEPFYNWRHIYISEEDKFSPFFGRQYSEFEFSLTVYNYYIH
                     PQWDDFGSRTLYLKVLLADYEEKYAVIELIGEWNDAVENDIMELKREVLEKFMYEGIH
                     KFILIGENVLNYHSDGKDYYEELYEELSDENGWIVCLNMPEQTQYDFKHARLNRYVEL
                     MELENWRTYKPFHLFKKIDNELTARLTN"
     gene            13933..14622
                     /locus_tag="PLO70_04190"
     CDS             13933..14622
                     /locus_tag="PLO70_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HQZ73686.1"
                     /translation="MKWSEQFTSSVGKKWVMALTGLFLIIFLVVHAGINACIWANDGG
                     EMFNKAAHFMGSTVLIRIAEIGLFLGIFLHILQGYMLEIQNRSKRKQGYAVPMGNKGS
                     KWYSRSMALLGTILLMFFILHWWHFWVPARFIGHEELTELEINGVQMHNMYNLMQFTF
                     QELWVVIVYVFACFSLCWHLIHGFQSAFRTVGVSNSKYVSLIQNVGIGYSVIISLAFA
                     MMPVSMYLGWV"
     gene            14753..16729
                     /locus_tag="PLO70_04195"
     CDS             14753..16729
                     /locus_tag="PLO70_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HQZ73687.1"
                     /translation="MLDSKIPAGPLEQKWTDYKGHVKLVNPANKRKLEVIVVGTGLAG
                     ASAAASLGELGYKVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIFRLFYDTVKG
                     GDYRARESNVHRLAEVSVNIIDQCVAQGVPFARDYGGLLNNRSFGGTQVQRTFYAAGQ
                     TGQQLLIGAYQALERQVALGNVHMYSRHEMLDVVMVDGKARGIICRNLVNGELERYFG
                     HAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAHKKGAFFGNPCFTQIHPTCIPVSGDHQ
                     SKLTLMSESLRNDGRIWVPKQKGDKRKANDIPEEERDYYLERRYPAFGNLVPRDVASR
                     AAKERCDAGYGVGTTGQAVYLDFKYNLINKYGKAEANKHGIENPDMETLIRLGKEVVK
                     EEYGNLFDMYEKITGENPYEVPMRIYPAVHYTMGGLWVDYELMTNVKGLYCLGEANFS
                     DHGANRLGASALMQGLADGYFVIPYTIGNYLADEIATKPIPTDHPAFTETEKAVSDRI
                     STLMNIKGNQTVESFHKRLGKIMWEKCGMARNEKGLQEAIVEIQQLKKEFWSDVKIPG
                     TIKEMNPELDKAGRVADFIELGELMCIDALNRNESCGGHFREESQTEEGEAKRNDADY
                     AYVAAWEYKGESSWELHKEQLEFEVAKPTQRSYK"
     gene            17147..17911
                     /locus_tag="PLO70_04200"
     CDS             17147..17911
                     /locus_tag="PLO70_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQZ73688.1"
                     /translation="MEQYTMNLTLKVWRQKNSAERGSFETYPVKDISSEMSFLEMIDV
                     LNEELIRDGKDPIAFDHDCREGICGMCSMYINGRPHGPWEKNTVCQLHMRAYKDGDTI
                     VIEPWRANAFPVIKDLMVDRSSFDRIIQAGGYISVNTGSPVDANAIPVEKNMADAAFA
                     AAACIGCGACVAACKNSSAMLFLSAKVTHLALLPQGHPERQTRAMNMVAQMDKEGFGA
                     CTNTGACEAVCPKGISIENIARLNREYMMAGLASDK"
     gene            18004..20166
                     /locus_tag="PLO70_04205"
     CDS             18004..20166
                     /locus_tag="PLO70_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQZ73689.1"
                     /translation="MYTKSISIAVVAIFLLVSCKNNDTVKKESVKWPEGVTAPVAEKK
                     IKELTAHGDTRIDEYYWMNDYFKKGTDSTKVVDYLKAENAYLDTMMSGTKKFQEELFA
                     EMKGRIKEKDESVPVLNNGYYYYSRTEEGKQYYKYCRKKGSLDAPEEILLDADKMAEG
                     AGYFSATGFSISPDNKLMAYGIDKLSRRQYTIYIKNLETGELLKDEIKGTGGNAVWAA
                     DNKTLFYTSNNPATLLSEKIRKHKLGSDANTDVTVYEEKDPSNYIGVYKTKSRDYIVI
                     YSSATLSSEYRIVKASEPDASFSLFQPRIKDVLYGIDHWGDKFLIRTNLDAKNFRLME
                     TPVDKTTRENWKEVIAHRTDVLLEDIDVFKGHWVITERKEGLLQMRIREIASGKEHYL
                     DFGEPAYAAYVGANPEFNTTNLRYNYTSLTTPNSVFDYDMNAKNKRMMKEQEVLGGFN
                     KADYVTERVYATAKDGAKVPVSIVYKKGTKKDGSAPLLLYAYGSYGASMDASFSSNRL
                     SLLNRGFVYAIAHIRGGQEMGRQWYEDGKLMKKKNTFTDFIDCGEFLIKEKYTSKGHL
                     YAMGGSAGGLLMGAVVNMAPDLWNGVVAQVPFVDVITTMSDPTIPLTTNEYDEWGNPA
                     DKDAYFYMKSYSPYDNLEKKNYPNMLVTTGLHDSQVQYFEPAKWVARLRVLKTDKNLL
                     LLKTNMEAGHGGASGRFDYLKEIAMEYAFLMALEGITS"
     gene            complement(20163..20720)
                     /locus_tag="PLO70_04210"
     CDS             complement(20163..20720)
                     /locus_tag="PLO70_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73690.1"
                     /translation="MKKSFALFLLLLPASFCFSQTGFQKKINPGGKKSVIAAGINIPL
                     GDFSNTHYGGFSTVYAWSKNRFGRLNSMPAKKIGFTANSGVAYYFGKKESIGAGSYKY
                     PAYIFLYAFAGAVYNAGKKIHFCLTAGPAAGIYSSNVQFNTGASLQCNYYISPKIAIT
                     PGLILMKETGSDAIWSGSLRAAMAF"
     gene            complement(20749..22890)
                     /locus_tag="PLO70_04215"
     CDS             complement(20749..22890)
                     /locus_tag="PLO70_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73691.1"
                     /translation="MRKSTIIFLSHFFICFALQAQQNNNWYFGQRAGIRFSGSPGQVT
                     PVTLTDGAMITDEGSAAISDSNGQLLFYTNGITVFNRQHQVMLNGNDLKGNISSCQAA
                     IIVPVPGNDSIYYIFTTDAIENNFSSGYNYSIVNMKRDNGNGEVVSKNTLLWQSCTER
                     LTAARHQNGTDIWLITNDNNSNIFRSWLITCNGLQSNPVVSQAGVILTQHLLTNTGYM
                     KISPDGKQLCQTHFPLFDPDVSNPNFVQLFDFDNTTGQVTNGRSVMFDNAMYVACEYS
                     SDSKMLYLVRPYERKIDQLEATLPSAAAVSASTISISTTSPVLTLQLAPNEKIYTFRP
                     GQSLGVINSPAVKGAGCNFQDNQLDISPFSARFGSPSFINDVAYDPNNSFSYVITDSC
                     SGRVQFTAISGMPGTLTWSWNFGDGNTSTLQNPVHTFAPANQSYTVSLTISSSVSCGN
                     IKSSRIIKPTGTITDADFDYVVRCDSGYIRFVNKSLFEQNGGGQYLWDFGDGNTSTAA
                     NPIHIYNREGFYSVKLKLLTGAACIDDSLIKQVEIKNFSVTAPPDQTITVGQSVILST
                     NEPADSFLWSPDTWLSNSNIRNPVATPLEDIVYKVTASNNEGCKGEDSVRITVLQYDD
                     IYIPTGFTPNDDGKNDILKPFYHGQVILKEFSIYSRWGQQVFSTSLRNAGWNGKVKGM
                     LQQAGVYVWFIKAIGKSGETIERKGTFVLIR"
     gene            23028..26498
                     /locus_tag="PLO70_04220"
     CDS             23028..26498
                     /locus_tag="PLO70_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73692.1"
                     /translation="MNQLCRIFLVITLALTSLWSSAQIPVAGNPVSNLRKKTFRIDAD
                     SIKVDTISIVPNTFFISNVVVEDYRIDYVKAILYWKKKPIADTVTITYRVFPFKLNPV
                     AQRMNYDSVMNNFYQKPFEFNNGLAGNQRGVFDFGTLKAEGSLGRQIGFGNSQDAVVN
                     SSLNLQLSGMLGDSIEIQAAITDNNIPIQPDGTTQQLNEFDQVFLQFKKRGWQLNLGD
                     IDIRQNQNYFLNFYKRLQGISFKTENRLSKSISSKTLVSGSIAKGKFNRQILDPLEGN
                     QGPYRLRGPNNELFFVVLANTERVFYDGQLLQRGEDQDYVINYNSAEITFTPRRMITK
                     DSRIQVEFEYAERNYLNTNLYLSQEFEINQKLKIRLGAFNNNDAKNSPINQVLDTRQK
                     QFLADLGDSTQNAFYPTALIDSFAADKILYEKIYVGLDSFYQYSTNPATAKFSLSFVD
                     VKQGNGNYVPEFNGANGKVYKYLPPVAGIKQGQYEPVQILVAPRRQQIVNLGIDYAID
                     KNTLVKAEVATSNNDVNTFSTKQDGDDRGWATKVQLSNKKMLRAAKKLELTTGLDYEY
                     VQSKFKPLERLRNVEFTRDWGLTLFSVPVNENIIRVSAGLKDKNNHAVSYQFINYNRS
                     DNYNGFQNVIKHNADWKGWRFNNQFTVTNFNVQNDKGTFLRPTLDLSKQLKKMDNWRV
                     GLNYALEQSTSRYKTNDTLTPTAFSFDIYSAYLKSDESKKNKYGITFFTRSDKYPVGK
                     EFVRGDRSLNLNLQTELLANPKRQFYLNTTFRKLKVYNATLSRQKEDETILGRAEYVM
                     NEWKGLLTGTVLYEVGAGQEQRRDFAYLEVPAGQGEYAWIDYNNDGIQQLNEFELAAF
                     PDQAKFIRVFTPTNDFIKANYITFNYRLDISPRAVLNSPNLKGAKKLLSKLNLLTSFQ
                     TSKKSVASGGFEFNPFKYGVNDTALITLSTTILNTLSFNRFSSKWGIDFSNYRNNGKS
                     LLTYGYESRKINDWSVKWRWNISRSLALTVNGKIGTNALYTPQFTNRNYQLSVYTVEP
                     SVIYIKGTKFRIVSSYKFDSKKNLPLYGNEKSISNSLNLESRYNILQRSSLTGKFTFN
                     NINYDSPANTTVSYIMLDGLLPGKNFLWSLGFNKRLINNLELNFQYDGRKAGSSRTVH
                     LGRAGVTALF"
     gene            complement(26561..27658)
                     /gene="ychF"
                     /locus_tag="PLO70_04225"
     CDS             complement(26561..27658)
                     /gene="ychF"
                     /locus_tag="PLO70_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQZ73693.1"
                     /translation="MALQAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIDPNVGL
                     VDVPDPRLNKLAELVVPDRIVPTQIEIVDIAGLVRGASKGEGLGNKFLANIREVDAII
                     HVVRCFEDENILRDEGAINPVSDKEIIDTELQLKDLDSVEKKIQKTEKQIKLDPKYKA
                     ELEVLIRCKEHLEKGKSIRELDLDKEEKNAIADLCLLTDKPVLYVANVDEPSMHTGNK
                     FSEALKESIKNEKAQMIIMNNNIEAQIAEMENGEDKQLFMEEYHMEEPALDRLIHSAY
                     KLLNLYTYFTAGVQEVRAWTIHQGWKAPQAASVIHTDFEKGFIKAEVISYDDFVQYGS
                     EAACREVGKLRIEGKEYVVKDGDVMHFRFNV"
     gene            27753..29153
                     /locus_tag="PLO70_04230"
     CDS             27753..29153
                     /locus_tag="PLO70_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQZ73694.1"
                     /translation="MALSRIWTAFIIIALLVAAGKFVFEPGQQHIFSSLVTGKNTDTI
                     VARTLDSAGIPASVHSQLIDQKVAIWGKEKVYRISDGGFRAYRLLHANGIFETTKDAV
                     NLCIGLIGIMALFMGFMNIAEKAGGIRFLSRIIGPFFSRIFPDVPKNHPSMGHMVMNF
                     SANLMGLDNAATPFGIKAMESLQEINPDKTTASNPQLMFLCLHASGLTLIPTIIIADR
                     VALKSQHPTDIFIPCMIATFAATIAAMLIVAYRQRIRIFQSVILLWVLGISAVIAALI
                     LYIKTLDAKGVQLFSTSLSNGIILLIFLLIILGAVYKKTPVFDAFIEGAKGGFETAIR
                     IIPYLVGLLVAISLLRNSGAFEFVMDGIKNLFIFFGGDIRVVDALPTAILKPLSGSGA
                     RAMMIDTLNAHGVDSFAGNLASVFRGSADTTFYIIAVYFGAVGIKNTRYAVTTMLLAD
                     LAGIITAIAMAYLFFG"
     gene            complement(29206..29946)
                     /locus_tag="PLO70_04235"
     CDS             complement(29206..29946)
                     /locus_tag="PLO70_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73695.1"
                     /translation="MKLINKTILGVASLAATFMFSSCTKSFDEKVEQNRDFSNSSLLQ
                     VYMATVNADRNYVYVDSKPVNGAKLILGSVFPSTGFAFAVPGGYRAFMIRDTLTATTQ
                     TPLSFAQNMDVGKNYTIFMYDTINAAKQKTVETKIVVPGDTTARLRFANFIFSKNAVP
                     AVDIFSKRRNVNVFTNVAVTGVTDFIPVPSAFTDTFYVRETGTTNQLAVLNGLTPTEK
                     RSYTLVFRGRYETTSGTIARTLSSFINR"
     gene            complement(29972..31609)
                     /locus_tag="PLO70_04240"
     CDS             complement(29972..31609)
                     /locus_tag="PLO70_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQZ73696.1"
                     /translation="MNKLLIKFCLLPVAAVLLFSSCSKKIDEAFLNPNATTRVPVETL
                     LPGIIGNFIGSSSAQGSAYGLANDGLYVGRYVQFWATNTPLNQYDRMSGATGGSDVLG
                     SVWAMHYYGMGQNLGRMIDWAAEEEKWDYVGVGHAIRAWSWLTLTNMYGEAILRQAFD
                     PNRLVFDYDTQEDIYAEVRRQAHLAIENLNKTGGGVSQANLALGDAYFYNGDVNKWKK
                     FVYSVLARSFHLTNKGANYQADSVLYYCNLAINSNADNAAAKFANTGITGTSNFFGPI
                     RGNVGVLRQTRFIADLMSGVNSMFLGVADPRAPYIIRENTNGTYKGVRPTKGPDGLAT
                     ADLPQNFWGSSFALTAAPGNDNPCRYIFQNGVQFPVITAAEIAFMKAEALWLTGNKPG
                     ALTAYIDGLNLSFDFLTGTPEFHNKVPAAMQITPATRAAYLANPAVVPTAANLTLSHI
                     MMQKYIALYGYGFMQTWVDMRRYHYTDLDPVTGMQVYADFAAPSGSDLYPDNNGKLAY
                     RARPRYNSEYLYNVAALTSIGAIALDYHTKEQWFSQP"
     gene            complement(31629..34820)
                     /locus_tag="PLO70_04245"
     CDS             complement(31629..34820)
                     /locus_tag="PLO70_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ73697.1"
                     /translation="MRKILRVFLALSLMLLLLPPSVQAQERTITGTILSEDSKTPLAG
                     VTVRVKGTRRITQTDANGKFTLKMNPGETLQVSYVGYVTTDVKPGSGETVGISLKTAD
                     NMMGEVVVTAMDIKRNPRSLGYSVQKVDGAEIQETQRENFLNSLGGRVAGLTMDPTSG
                     VAGASTSVVLRGYNSLSLSNQPLYVIDGIIMDNSTINETSGGGQDIGLASDRPNRNTD
                     YQNRIADLNPSDIETITILKGPEATALYGSQASSGAIVITTRKSKTNKLGIQYDNSFR
                     VQKVTRWPETLDEYENGINGIPSNVFTYFGPAYAPGTQLFDNKDAFFRNGTSQTHNLG
                     ADFGFKESKFRVSGSYFDQKGVVPENDFKRYTFRISNTTKLFKGKLELSPSFAYTRSD
                     NNKVLRSAGGYLLSLLIWPSTNDIRSFEDPNGDKLALFNSNPNADFDNPLFNVKKNRS
                     YDETDRKTYSLGVNFNPFSWLSLTGRFGYDTYDMVGYQFYHPQSFYLARGTGGTLDNY
                     WRKYIGYNHTITATVKKKIGKDLELRLMGGTMWQDYETKMFAIYGTNIVDSVGGVTGK
                     MYKNGTILTDNDLRGVVGDYMDSSITRTNTRLRLLQNNLGRYNQSISRQFAVFGEFAV
                     SYKNYAFFSYTHRFEEASTLPEKNRKYDYPGGSLSLIVSDIFPALKKNSVLSYWKLRT
                     SLAGTARLNPPYSTQSVFVNNFASGGGFSYGFFNNNADLAPEKQKTYELGTEFRLFSS
                     RLNVDMTYYNTLNKGQIINAFRLSYATGFVLNTQNAASTRNQGFEVALNFDMLQKKKA
                     LQWNMRLNFNKMWNKVEDMPRNVAEYYIADTWLYGNARGGLVFGGPTTTITSFGYARN
                     NAGQILINPLNGLPVVEGLFKVRGDRNPNFTLGWLNSFSWKNWKLSMLWDLKVGGDIF
                     NGNNMFLTRTGRSKLTADRLLPRVIQGVLNDGLQNSATPTPNTIAITPYYQQDYYTTM
                     PEEAFIEKDVNWFRLRDISFSYTFSSNFIKGIKSLSAFVTGNDLVLLTNYSGADPMVN
                     GNTSGSRGVGAYGFDYGTLPAALTVNFGIRASF"
     gene            complement(34868..35617)
                     /locus_tag="PLO70_04250"
     CDS             complement(34868..35617)
                     /locus_tag="PLO70_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQZ73698.1"
                     /translation="MLKKERQAYILHQVNLHNKVLSSSLCTEIKVSEDTIRRDLQELA
                     EEGKVIKVHGGALSHSFNDVSLPLNGVYSQNQKKTIAQKAISLINNGMFVLTSGGTTI
                     MELARSLPPQLKATFISGSIPVILEYMYHPNIEVILIGDKISKNSKITVGSEAIAKIK
                     QMKPDLCFLGTNAIDVKHGITDNDWEVVQLKKAMIESSKKVVCLSIAEKINTVQPIRV
                     CGIDEIDILITELNENDPVLRPYKDAGLKVL"
BASE COUNT        10526 a         6896 c         7779 g        10618 t
ORIGIN      
        1 ttcttatttt gttttgaatc gtttttaatt gctcaaattc aaattgaatt tttgcgttca
       61 gttctgttcc ttcaacagtc ttccctacca tatttgcaat ggaatggatc atttcatacg
      121 catccttcag gttatttata tcacttaccc aaactggata ccgggttgcc agttcttcta
      181 cctgttcttt tatattttct tctttattgg cgatgatgag gtcgggatgc aactgatgga
      241 tcaaatccgt ttttaattgc ttagttcctc caattcttgt tttggtgcgg aaccattgtt
      301 ccgggtgtac gcagaatcgg gtaatgccga ttacttcttt gtctaatccc aggtaaaaca
      361 ataattctgt ttgtgacggg accagggaaa taattcttgc cggagttttc tccagggtaa
      421 ttgttctgcc tgtttggtct ataaaagtag acatgcagta ttttaccgca aattaatgat
      481 gaatacgctg tgttgaaaaa tgaaaagagg aagggagaaa aaggttcacc gcgcctcctg
      541 ctaaatttta gtttagcagg agggttccgg acgtaagttt ctttcaaggg gatttggaca
      601 cggtgacact tggtcggttt tcaaggatct cttttggact tctttggttt ttcgatcgga
      661 tctggacttc aattggtttt tctggaaatc tggatattgt tggtctttct aaggattcgg
      721 ttttttaatt cttcaataag tattggatga ttggagaaca aaaataattg gcaattcatt
      781 aataaaacag agcaaaataa cctctttttt ttacgacagt ttttaccttt tttatcgtaa
      841 agccgccact gccaggttgc ggatttactc agttaattta tcgtagaact acgaatacag
      901 cctgtacaac agttgccagg cttattttaa aacaatagta aaggtttatt gcattagttt
      961 cccaatgcct ggataacatc atactttgta acgatatggt aattgccggc ctcatcttta
     1021 gccagcaccg ccccattttc tttattaatc agatggctta gtttctcaac cggtgtatca
     1081 aaagccacaa tcgggaaaga aggttcgagt acttcctgta cagttgaatt ttttatttcg
     1141 gggttatcaa atattttctg aaacaaacct ccttcgctga tggcaccaac agaaccattt
     1201 tcattcatta caggtatttg ttcaatgtca tattttttca tcagttcaac tgcttccaaa
     1261 actgtttgtc ccggttctac agtgatcaaa cgtttggtgc ctctggaact taccacatcg
     1321 cggaaagttt ttacatcgag gaagccccgt tctatcatcc actgatcatt atagatttta
     1381 ccaacatacc ggctgccatg atcatgaaaa atacaaacaa ccatgtcctc ttttttcaaa
     1441 cggtctttca attgcaataa accctgtata caactgccgg cactgtaacc acagaataat
     1501 ccttcttctt tagccaggcg tcttgccatc actgctccgt ctttatcagt tacctgttca
     1561 aaatagtcga tgacactcat atcataattc ttgggtacaa agtcttctcc gaaaccttcg
     1621 ctgatatagg gatgtacttc gttcatgtcg atctcgccag tacgaaaata tttggtaagc
     1681 aaagagccat atacatcaat agcccaaatc tggatgtttg gatttttttc tttcaggtat
     1741 tgtgcagttc cggtaatggt gccgcctgtt cctgcggtac ataccaggtg tgtaattttt
     1801 ccatcagttt gtttccatat ctcagggccg gttgtttcat aatgtgccaa acggttggcc
     1861 aggttatcat actggttcat gtggaaggag ttggggactt ctttggccag ccgggctgct
     1921 acactgtaat aacttctcgg gtcctccggt aatacattgg ttgggcaaac gatcacttca
     1981 gctcctacag ctttcaggat atcagctttt tctttagatt gtttttctgt ggtaacaaaa
     2041 atgcatttgt acccttttac tactgcagca agggctaatc ccatccctgt attaccgctg
     2101 gtgccttcaa tgatggtgcc accgggtttc agtttgcctt ctttctcggc tacttccacc
     2161 atctttaatg ccatccggtc tttgattgag ttaccgggat tgaaatagtc aacttttgct
     2221 aacaccgtac agggaagatc cctggtgatt ttattcagcc tgatcaaagg tgtattccca
     2281 attgtctcga gtatattatt ttttatatcc atagtctttg ttgaaattgc aaatttacaa
     2341 aacgatatca tcctgattct aatcaggcca gttaaaaccg cctccctttt ctttatcaat
     2401 agccagaaat tgggtcttta atagcttagc tcttgcggcg attaaagcag gaaacccggg
     2461 attggctttg aaaagtttga aggtttttaa tacgccctct ttaaataaca gatggatacc
     2521 tttgttataa taaaggtatt cgtcatatcc cgggtaaggg gttttgataa actgatcacc
     2581 aaagtatttt ttacatttct ctgcatcatc ttcaaatcgg atgttctcat taataagtac
     2641 tttctctgcc agagtattgt ccggtaaata agaagtaaca atcatgcagt ttactttgcc
     2701 ctttaacaga aaaattttaa atatgggcca tttcctgttt aaatcatcat ccaggacaag
     2761 gcatttgtca aagccaagaa caaactgggt aatgctgtct atggccactg tccggttatg
     2821 atgccagaaa gcggtcattt cttctttgaa tgttttttct tccatcgaat cgcccaggat
     2881 agcttttacc tgtatttccg tcatcccgat actgatatct ccgcagccaa tccctgcagt
     2941 tacagaagtc gtttgttgtg ccatcaatac aaatgagaaa ataactccag cgaaaagggt
     3001 ggtaatttgt ttcatatgtt aagctccaaa ggggcgaaga taaactaacg cctgttactt
     3061 tttgactcta aaataaaaag cccctcctga taccagaagg ggcctaccgg acttaatgat
     3121 aatttatcat caagcttctt ttctgaattg ttttctttta ttactgaaat aattatccag
     3181 taaaaccagt cctaacaaga cattgatcat cataaatgca ttaacccatg tattattaaa
     3241 gaatttagtg taatctattt tacttatttt atcagtaatg gcactgcttt cacctgtact
     3301 ccaatccatt tgactaaaac cgtaaacaag aatagcaacc agcatggtca tgaaaaataa
     3361 accaataccc cagataattc ttttattgat ataggatttg gtagcggggg caatatgcat
     3421 tttcgctatc tcttccataa cattttttgt aaaccgcata gagggtgcct ccagctccga
     3481 tgattgaagc atctcattta cctccagcag ttcactgtat tttgctttcc attcagtatc
     3541 tgtctgcagt aatctttcta tgactgtttt ttcttccagg gaagaatggc catcaataaa
     3601 ttcccacaaa cgttcttcga tatttcgttg cgtattcata caattcaatt taaatctttt
     3661 acttcatgtg caaaatgagt ttccatcttt tcctttagtc ttgttcttgc ccggtgtaac
     3721 cgcacctttg ctgtattaac ctccaatccc aggatttgag caatttcttc gagtgactgt
     3781 tcacttttat aaaacaatgt aataatctcc gcatcgtctg gccccattaa accaatagcg
     3841 ttatgtacca ttgattgtct tgatttttgt tccaccaggt ttgccctgaa tcccgaatcc
     3901 atgctgtcgg ctacctcaaa cactttttca ttatccagcg aatgaatctc cagcttcttc
     3961 tttctcagga agctgataca ggtgttgttt acaatggtat acaaccaggt gctgaactta
     4021 gaggcacccc ggaagtcagc caggtattta aatgctttaa taaatacatc ctgagccact
     4081 tcctcagcat cctccctgtt ttttaccatt cgcagcgtta gtgtgaaaac gtagttctgg
     4141 taacggttca ccaacccggc atatgcctgg tgatcaccac ttagcacttt gctaatgagt
     4201 tcattatcat ttagtccggt agacatcctt atataagact acacgataag tttttaggtt
     4261 acaatacaaa ttacgattaa ttgtccctaa ctccggaagg ggaaataaga aagtttagaa
     4321 aaagctgtaa ccgggtttca ccggctgtag tcatagttac tgtaaaccga tttgaaaaaa
     4381 agttcctgaa ggctgtaacc cggatcaaat accggtagtc ataaaaggac aaacaaacaa
     4441 aaattaataa taataaaaac taccattatg gacgcaggag aagttttaat accgattaca
     4501 atgtttgcag gaggatttgc aatgattttt ggtatttact atttaaaaac ccgccagaac
     4561 ctggcaatga ttgagaaagg aatgaacccg aaagagtttg ctaaccggcc ggctccttat
     4621 aaaaatctta aatgggcatt attgctgatt ggggctggta ccggtttatt cctggcatat
     4681 ttactcgaca attacatgct accagtggag atcaacaagt ttggtaatca tggtgacaga
     4741 aatgtaccta tttactttgc ccttatagcc atcggcggtg gtcttggatt gtttggttct
     4801 taccgaattg aaaagaaatg gtgggacgat aataaagaca agcaataacc tcaccacaca
     4861 cattaccgga agcaacagcg aaaactgttg ctttttttat tttgcaattg tctccattgc
     4921 tttttttacc atagcatcat tgctgttcag tacctggtaa aaaccggcat ttctccattt
     4981 gtaccgggcc agcaatgctt ttaatctttg ctgcactaac tctttttctt ttcctgctac
     5041 cgatttaaag tttacagagt cggttgctgt gaattttacc aggtcgttcc agataccttc
     5101 tgtattgtta aacccggtga tgaagtcagc tgtggtgttg tatttatcta cctgtatttt
     5161 gttctggagg taataattgt aaacaaagct gttaatattg gcagcaaaag tgagcctgtt
     5221 tgccttttgt tgcgacacgc tggtatcaat aggaacaaaa atggagggag tgatgccgcc
     5281 gcctccatac aatgtgtcac tgcatgctgt ggtgaaacga aatccattat gatttttcat
     5341 cgagtcagcc gaaaccattt ctccgctgtt gaaccgttcc cataattcat ccatatacac
     5401 ttttttgcct ttttcgtagg gtcgctggat actgcggcca aggggtgtat agtaccttgc
     5461 taccgttagc cgcatggcag atccgtctct cagctggtac tgttcctgta caaggccttt
     5521 accaaaagtt ctcctgccta ttatggtagc cctgcaccaa tcctgcaagg cgccggctaa
     5581 aacctcactg gcactggcag ataattcatc caccagcaag accagcttcc ctttctcaaa
     5641 aagaccaggg cgtttgcagc ggtattccct ttttttgcta ttgacacctt ctgtatatac
     5701 gatcagttta tctccttcga gaaattcatc tgccatgtca actgcctcat tcataaatcc
     5761 accaccatta ccacgcaagt caaaaacaag acttagcatt ccctgttttt gtaatgcttc
     5821 catcgcctgc atgaattctt cgtagctgct ttcactgaac tttgtgagtt tgatataacc
     5881 ggtgttttta tcgatcatat aagaagcctc aatagaggga acaggtatct ttcccctggt
     5941 aacagtaatg gattgttgtt tagcacctct tattacctgt aatatggctt tggagccttt
     6001 gggtccgcgg atcaaagctt tgatctcatt aatggagact ccttttcgtg ttagtgaaga
     6061 gtcatttacc ctggttattt tatcacctat cactaaaccg gccttatcgc ttggtccgtc
     6121 agggaaaacg taaacaacat taactgtatc actgaaaata ttgaactcta caccgatccc
     6181 gtcaaagttt ccggccaggt cttcattagc ttcttttagc tccaccggag gcaggtacac
     6241 tgagtgaggg tccagttcgt tcatcatttc ctgaatagca cggccttcca gtgtatctat
     6301 ctgcaccgag tcgacatatc gcatttttat caggtcaagt gcttcctgca gggtactgcg
     6361 gctattggaa gaaaaaaagc ccctttttga accagactga tcacttaatt taaagcccag
     6421 gaacattcct cctattaata ccagcgaaaa aataagcgga agccagacct gcaatttttt
     6481 atttcccatc ataaaccttt attcagtaat ttgacgcaaa atactggatt tgaacgtaac
     6541 agaagttgtc gctcatccaa ttcaacaaat ctttatatgc cggttgtaaa attgattgca
     6601 gacagcgggg ccacaaaagc cgaatggtgt ttacttcaga atggcaggaa aaagaccatc
     6661 attacccagg gtatcagtcc ttactttctc aatactgaac agataacgga tcttttattg
     6721 aaagagctaa agccaaaact gaagaataca aacattgacg aggtttacta ttacggtact
     6781 ggttgtgcca accctgtcaa tgcaaagtct gtaaaaaaag ccatcagtaa agtttttcag
     6841 catacaaaag tagaagttac gcatgacctg atgggtgccg caagggcctt atgcggcaga
     6901 aaaaaaggag tggcctgtat tttaggtacc ggatcaaatt cctgctacta tgatgggaaa
     6961 aagatcgtaa aaaacagtcc ggggctcggt tatgtattgg gagatgaagg tagcggtgcc
     7021 tacctgggca agaaagtaat tcaatattat ctctacggaa catttgatga tgagctacgg
     7081 ggaagatttg atctcactta tactaccaat gcgtcggaga tactggagaa tgtttacaag
     7141 aaacctttgc ccaatcgcta tttagccggc ttcgcaaaat ttttggcgga aaacaggggc
     7201 cattatatga tagagaatat cattgaagac gggttgaatg attttttctt caatcatctt
     7261 tgcaagtacc gcgaaacatg gaccttgcct atccattttg caggaagtgt tgcatttggt
     7321 tttaaagatg tgttgcagca actttgcaac agttatgaat ttgaattggg cagggtgatg
     7381 aagaacccga tggaaggttt gatagaatat cacagttaaa gaaaggacac atggctttta
     7441 aaaaaattac tgagcagtca agcaattacc gtcacctgga aaaaatgaca gtgggtgaac
     7501 tactcaccaa tattaataat gaagataaaa ctgtgccgga ggcagtagaa agagcaatgc
     7561 cacagataga aaagcttgct gaaattgcca gtgataaaat gctgatgggg gggcggttat
     7621 tttatatcgg tgccggaacc agcggcagat taggaatagt ggacgccagc gaatgtccgc
     7681 caacttacgg agtgccatac gggttagtga ttggaattat tgcgggtggt gaaaaggcga
     7741 taaccactgc cgttgaattt gcagaagatg acagagaaca gggttggatg gatttgcttg
     7801 aacataaagt gtcagacaag gatgtggtga tcggaattgc tgccagtggt actacaccct
     7861 atgttattgg tgcattggat gaatgcagga aaagaggtat cgtcaccggc agcatctcct
     7921 gtaatcctga ttcgccagtg agtgctgcgg ctgatttccc gattgaagtg gtggtgggac
     7981 cggagtttgt tacaggcagt acgaggatga aaagcggaac cgcccagaag cttgtcctga
     8041 atatgatctc gaccactgtt atgatacagt taggacgggt agaagataat aagatggtaa
     8101 atatgcaact aacgaacgat aagttggttg accgtggtac caagatgctg atggagagag
     8161 tgaagatcgc tgattatgag aaagcaaaag aattacttct taagtacgga agcgttaaaa
     8221 aagcagcaga ttcttttgta ggataatttt atttttcctt tttgcaacgt atattgtgct
     8281 tctgatttca aaaagtggta aaaacagtta gtcatatatc ttcgttcctg gtgatgaatt
     8341 tcaccatcac cactattatt actacaaccc gttaagggtt gtatattttc atatcaatac
     8401 atgggcagtt tgctgtaaga tgacggaagt cgcttattgc ttcacattta tctaatcatt
     8461 caaaattcag gtcatgcagg tattaaagtt cgggggtact tctgtcgcta atgcagaaaa
     8521 tataaataaa gttgttgcga ttgtaaagga gtcggttaaa aaagaaaaaa caataatagt
     8581 agtatcagca ctgggaggga tcacagattt attactggga gcggcagcaa tagctgcaga
     8641 gggaaatgaa gcctacaaag aaaaattatc acaggttgag cagcggcacc tggacaccgt
     8701 taagcaattg atccctgttg cacaacaaag ccagttattg agcctggtaa aaaaatcatg
     8761 taacgaaatt gaagatatct gcaatggcat ttttttgcta cgggaattaa ctccaaggtc
     8821 aaaagacagg attggcagtt atggggaatg gatctcttcg cagatcattg ctgcaaagtt
     8881 cattgcagaa gggctcgatg ctgtatggaa ggattccagg gaattgattc ttaccaattc
     8941 tacgtttact gcagcagaag tggatttccc gattacgaat aaaagaacag gggagttctt
     9001 tgccgcagct tcttccactc tttttatctt acccggattt attgctgctg acaaaaatgg
     9061 aataactaca actttaggaa gaggagggtc tgattttact gctgctatta ttgcttccgc
     9121 tgttaatgca tccgttttgg agatatggac tgatgtcagt ggtatgatga cagccgatcc
     9181 gcgactgacc agtaatgccc ggatcattcc gcatatatct taccaggaag cgatggagtt
     9241 gtcgcatttc ggtgccaaag ttatttatcc gccaactatt cagccggtga tgggtaagcg
     9301 gatacccgta tgggtgaaaa atacatttgc tcctgctgat gcaggaacat tgatcgaatc
     9361 agtagccaca aaagatgggg atatcgtaag aggtatttcc agtatcaata atatagcatt
     9421 gatcagcctg gagggaagtg gtatgatcgg tatcccggga ttttcaaaaa gattatttga
     9481 agcattaagt agtgaaagga tcaatgttat attaattaca caaagctctt ccgagcattc
     9541 catttgcgtg ggtattgatg cgctgtctgc agcaaaggca aaacaagcgg tagattcggc
     9601 ttttgccaat gaaatagcgt tggaaaaagt tgagccgcta caaattgaat cagcactttc
     9661 tattgttgca ttggttggag agaacatgaa aagtcatccc ggtatcagtg gccgtatgtt
     9721 cagtgccatg gggaggaatg gtgtgaatgt aagagctatt gcacaagggt cttcagaaaa
     9781 aaatatttca gctgtcattt ctaccaacga tgtacgtaag gccatcaatg ttttgcatga
     9841 agagtttttt gaaacaacgt acaaacaggt aaaccttttt ataaccggaa ccggaaatgt
     9901 cggagcaaaa ttattgggtc agttacagaa gcaacttgaa ttcctgcagg agcaattaca
     9961 tttacaggta agagtagttg gtttaagtaa cagcagaaag atgctgattg atgaagaagg
    10021 tattgattta aaaaactgga aagagaagct cgaagcaggt gaaacggcca gcctgcaaaa
    10081 actaacagac gaaattatca gccgtaatct ccgtaattct gtttttgttg atattaccgc
    10141 taatgataaa gtggccgggg tatatggttt gttattacag aaaagtattt ccgtggttgc
    10201 ctgtaataaa gtagctgcct cttcagccta tagtaattat aagaaattaa aggacctggc
    10261 cagggaattc aacagccagt tcttgtttga aactaatgtt ggtgccggtc ttccgataat
    10321 tgctaccctg aatgacctgg tacacagcgg agacagggta cataaaatcc aggcagtact
    10381 cagcggtaca ttgaattttg ttttcaataa ttacgatggc agccggaaat ttgcggaggt
    10441 ggtaaagcag gcacaggagg aagggtatac tgaaccagac ccgcggcttg acctgggagg
    10501 aacggatgta atgagaaaaa taatgatcct cgccagggaa gcaggacagg aaattgagat
    10561 ggacgatata gtaaataatt cttttatgcc ggcgagttgt atgcagggat cagtggatga
    10621 tttttatgca gccatgctga atgaagaagc gcatttcaaa aaactggcag aagatgcgaa
    10681 cattgcagga tgcaaattga aatttgttgc ctcatatgac aatggtaaag catcggtagg
    10741 tttgcagcat attgatccga agcatgacct gtatcacctg tacgggaaag acaatgtcgt
    10801 tcttttttac actgaccggt ataaagaaca gcctttagta gtgaaggggg ccggtgctgg
    10861 tgcagaagtg acagccagtg gtgtatttgc tgatattatc agggcatcaa aagtataaaa
    10921 tgaagaaagt taaagtacat agtccgggta cggtagccaa tattgtatgc ggatttgata
    10981 ttcttggtct tgcattgagt gaaccatacg atgtgatgga agtgatgctg cttgaccggc
    11041 ctgaagtgat tattcataat agggacattt ttaatttgcc gacggaagca gaaaagaatg
    11101 tagccggagt tgtattatta tcggtaatgg agagaatgaa taatactatt ggatttgaag
    11161 ttactgttga aaagcacatc aaaccaggaa gtggtattgg ctccagtgca gccagtgctg
    11221 ccggggcggc aatagcagcc aattatttat taggaaatat tttttccagt gatgaagtgg
    11281 tgcagtttgc gatgaacggg gagaaactgg cttctggtgt aaaacatgct gacaatattg
    11341 ctccctgtat tttgggtggc atcagcttga tacgttccat tcatccactg gatatagttt
    11401 ccattccggc acctgactta tttgtcacag tggttcatcc gcagattgaa gtaaggacat
    11461 ctgatgccag gcagatactg aaacaacagg tgctcttaaa agatgcaata aagcaatggg
    11521 ggaatattgc cggcctggta acaggattta tgaagaatga ttatgatctg ataggccgtt
    11581 cactcgagga tgttatcatt gaacctgtac gaagtatcct tattccggga tttgatgagt
    11641 tgaaagtaaa atgtaaagag gcaggagcat tgggtggtgg tatttctggt tccgggccat
    11701 ctgtatttat gctgagtaaa gatgaatcaa ctgcgaaagc tgtcgggcat gtgatgagcc
    11761 aggtatatga acaaattggt attgattaca atatttatgt aacgactatt aataggaaag
    11821 gggtagaggt ggtgagcagc gagtagcaga atgcagggtt ctaattaatt tatttaatgc
    11881 tttcttctaa atgaaatatt acagtacaaa taaaattgca ggatcggttg atttcaaaga
    11941 ggcaacgatc agagggcagg caccagataa agggttatac tttccggaga tcattccatt
    12001 tgtggataag gatatgatca gcaatattga cagctataca aatgaggaaa tcgcattcag
    12061 gataatagca ccttatgtag gtaacgggat tccagaagac aggttatggg aaatagtaaa
    12121 ggaaacagtg aattttgaga ttccgctggt gaaagtgaat gatactattt cttctttaga
    12181 attgtttcat ggcccgacgc tggcatttaa agatgtgggt gccaggttca tgagccgttg
    12241 tttgggatat tttgtacaag gagattcaag aaaagtaacc gtgctggtgg caacatccgg
    12301 tgataccggt ggtgctgttg ccaatggttt ttataatgtc gaaggggtag aggtggtcat
    12361 actatatcca tccggcaaag tgagccccgt acaggaaaaa caattgacaa caccgggtaa
    12421 aaatattcat gcgttagaag tgcagggtac ttttgatgat tgccagcaaa tggtgaaaca
    12481 ggcttttgcg gataaggagc taacggataa attatttttg acctcagcca attcgataaa
    12541 tgtagcccgg tggttaccac aacagtttta ttatttctat gcgttcaagc aatgggaaca
    12601 aaaaaataac ccgcctgtaa tttctgttcc cagtggcaat tttggtaata tctgcgcagg
    12661 tatactggcc atgcaatccg ggttacccgt aaaacacttt attgcagctt gtaatgtgaa
    12721 tgatattgta agtgaatacc tgcaaacaca ggaattgcaa ccaaaagagg ccgttgccac
    12781 tttgagtaat gccatggatg taggtaaccc cagtaatttt gtacgcatac tggagatttt
    12841 taagcaccag tttccattgt tgaagaataa gctatccgct tacacgataa cagatgctga
    12901 aacagtatca acaatcaggg aggtgtacga aaaatataat tatattcttg acccgcatgg
    12961 tgctgtcgga tatctttctt tacaccgtta cctttctgca catcccggcc agaccggatt
    13021 gtttttagaa acagctcacc cggttaaatt tccggaagct gtggaaagct gcattggtaa
    13081 gtgcattgaa atgccggcag ccgttcagtc tcttatggat aaagaaaagc aaagtgaatt
    13141 aatcaaagct gactatcagc aattcaggga ttatctttta aaataagatg cctttctgat
    13201 tattgctttt tctactttat cacttagctt atctttgcac tgagtagcag gtttatgcat
    13261 aatttagaac ccttttataa ctggcgtcat atctatatca gtgaggaaga caaattttct
    13321 cccttcttcg gccgtcagta ttctgaattt gaattttccc tgactgttta caattattac
    13381 atacacccgc aatgggatga ttttggcagc cgtacgttat acctgaaggt gctgctggca
    13441 gattacgaag aaaaatatgc cgtcattgag ctgatcggtg aatggaatga tgctgtggag
    13501 aacgacatca tggaactcaa aagggaggtg cttgagaaat tcatgtacga gggtatccat
    13561 aagtttatcc tgatcgggga gaatgtattg aattaccata gtgacggaaa agattattat
    13621 gaagagttat atgaagaact atcggacgaa aatggctgga tagtttgttt aaacatgccc
    13681 gagcagaccc agtacgattt taaacatgcc cgtctgaacc ggtatgttga gttaatggaa
    13741 ttagaaaact ggcgaaccta taagcctttt catttgttta aaaagatcga taatgagctg
    13801 acagcaagac taactaatta gtcatttata tgcgatgggc ttatgatttt tgtcatttaa
    13861 aacttttatc tgtttttggt cacggggcat tacttttgca gaaattgatt acttcctcta
    13921 agaatattgc ttatgaaatg gtctgaacag tttacttcct cggttggcaa aaagtgggta
    13981 atggcattga cgggtttgtt ccttattatt tttctagtgg tgcatgccgg tatcaatgcc
    14041 tgtatctggg cgaatgatgg cggtgaaatg tttaacaagg cagcccattt tatgggaagc
    14101 actgttctta tccggattgc agaaattggt cttttccttg gtattttcct gcacatcctc
    14161 cagggctaca tgctggaaat acagaatcga agtaaaagaa aacaagggta cgcagtacct
    14221 atgggaaata aaggcagcaa gtggtacagt cgcagcatgg cattattagg gacgattctt
    14281 ttaatgttct tcattttgca ttggtggcat ttctgggtgc cagcccgttt cataggacac
    14341 gaagagttaa cagaattgga gatcaatggt gtgcagatgc ataatatgta caacctgatg
    14401 caattcactt ttcaggaatt gtgggtggta atcgtttatg tatttgcctg cttctcttta
    14461 tgctggcatc tgatacatgg ttttcaaagt gcattccgga cagtgggtgt aagtaattca
    14521 aagtatgtaa gccttattca aaatgtaggt atcgggtact cagtaattat ttcactggct
    14581 tttgcgatga tgccggtaag tatgtacctg ggatgggtat aaataaccat tacgaattaa
    14641 ttgcggtaag gtatgatgtg tttgaaagtt agaatagtta ttacaggcaa agatcttaat
    14701 agcgaactgt aaaaaagcaa ataaatcatt tatatcaaag taatcagagg ttatgcttga
    14761 ttcaaaaatt cccgccggac cgttagaaca gaaatggaca gattataaag gacatgtgaa
    14821 actggtgaac ccggctaaca aacgaaaact tgaagtgatc gtagtcggta ccggtttggc
    14881 tggtgcttct gctgcagctt ctttaggtga gctgggatat aaagtgaaag cattttgttt
    14941 ccaggattca cccaggaggg cacactctat tgctgcacag ggtggtatta atgccgctaa
    15001 gaattaccag aatgatggag actctatctt ccgcttgttt tatgataccg tgaaaggggg
    15061 agattaccgt gccagggaat ccaatgtgca tcgtttggcc gaagtgagtg taaatattat
    15121 tgaccagtgt gtggcacagg gagtaccttt tgccagggat tatggcggat tactcaataa
    15181 ccgttccttt ggtggtacac aggtgcaaag aactttttat gccgcaggac aaaccggtca
    15241 gcagttattg attggtgcat accaagccct ggaaagacaa gttgccttag gtaatgtaca
    15301 tatgtatagc cgccatgaaa tgctggatgt ggtgatggtg gatggaaaag caagaggcat
    15361 tatctgtcgt aatcttgtaa acggtgagct cgaaagatat tttggtcatg ctgtattgct
    15421 ttgtacaggt ggttatggta acgtattcta tctttctacc aatgccatgg gcagtaatgt
    15481 gacggctgcc tggaaagcac ataagaaagg tgctttcttc ggaaatccct gctttacaca
    15541 aattcatcct acctgtattc cggtgagtgg tgatcaccag agtaagttga ccctgatgtc
    15601 tgaatcgtta agaaatgacg gtcgtatctg ggtacccaaa caaaaagggg ataaaagaaa
    15661 ggcgaacgat attcctgaag aggaaaggga ctattacctc gagagaaggt atcccgcatt
    15721 tggtaacctg gtaccaaggg atgtggcatc aagagcagcc aaagaaagat gtgatgcggg
    15781 atatggtgtc ggaactaccg ggcaggccgt ttatcttgat ttcaaataca atctcattaa
    15841 taaatacggg aaggcggaag caaataagca tgggattgaa aatccggata tggaaacatt
    15901 gatccggtta ggtaaggaag tcgtaaagga agaatatggg aacctgtttg atatgtatga
    15961 aaaaattaca ggggaaaatc catatgaagt acccatgagg atttacccgg cagtacacta
    16021 taccatgggt ggcctctggg tggattatga actgatgacg aatgtaaagg gattgtactg
    16081 ccttggtgaa gctaacttta gtgatcatgg tgcaaatcgt cttggggcat ctgccctgat
    16141 gcagggattg gcagatggtt attttgttat tccttacact atcggcaatt acctggcaga
    16201 tgaaatagcg accaaaccaa ttccaactga tcatcctgca tttacagaaa ctgaaaaagc
    16261 ggtgtctgat cgtatcagta cactaatgaa tattaaaggc aatcagactg tagaaagttt
    16321 tcataagcgt ttgggaaaga tcatgtggga aaaatgcggg atggcccgca atgaaaaagg
    16381 actacaggaa gctattgtgg agatacagca attgaaaaaa gaattctgga gtgatgttaa
    16441 aattcccgga actatcaaag aaatgaatcc tgaattggat aaggctggtc gtgttgccga
    16501 ttttattgag ttgggtgaat tgatgtgtat agatgcatta aacagaaatg aaagttgtgg
    16561 tggacacttc cgtgaagaaa gccagacaga ggaaggggaa gcaaagagga atgatgctga
    16621 ttatgcgtat gtggcagcat gggaatacaa aggagagagt agttgggaac tgcataagga
    16681 gcaattggag tttgaagtag cgaaaccaac tcaacggagt tataaataat tgaaacaatt
    16741 tggtaattcg gcaatttgcc aatgcaggtt caaactgaaa atattattct taaaaaaaca
    16801 attgaatttt cattacttgc tattgaatat gttgagttac ttgaaagtca caagagatat
    16861 gttataacag gcaattatta aggtctgcaa cttcaattgg tgcaaatgtt catgaagctc
    16921 agaatgcgga aagtaaagct gactttattc ataagttcaa aattgcggca aaagaagtag
    16981 aagaaacaaa gtattggctg gtattatgta atgagtcaaa cctgtatcct gattgcaggc
    17041 agttggttga taaattacat gaaattgata aaattattac gaaaataata tcgacaagca
    17101 aggctgggta attgccgaat tgtctaattg acaaattgaa taaattatgg agcaatacac
    17161 aatgaatctt actttaaaag tctggagaca gaaaaatagt gctgagcggg gcagttttga
    17221 aacgtacccg gtgaaggaca tttcttctga aatgtcattt ttagagatga ttgacgtatt
    17281 gaatgaagaa cttatccgtg atggaaaaga cccgattgcc ttcgatcatg attgtcgtga
    17341 aggtatttgt gggatgtgtt ccatgtatat taatggacgt ccgcatggac catgggaaaa
    17401 gaacacagtt tgccagctgc acatgagggc ctataaagat ggtgatacca ttgtgattga
    17461 accatggaga gccaatgcct ttccggtcat taaagacctg atggtcgatc gttcttcttt
    17521 cgatcgtatc atccaggcag gggggtatat ttctgtcaat acaggaagcc cggtggatgc
    17581 taatgccatc ccggttgaaa aaaatatggc agatgctgct ttcgcagcgg ccgcctgtat
    17641 tggttgcggc gcttgtgtgg ccgcctgcaa aaattcatca gccatgctgt tcttatctgc
    17701 caaagtaacg catcttgctt tattacctca aggtcatccg gaaagacaga ccagggcgat
    17761 gaatatggtg gcccagatgg ataaagaagg atttggcgcc tgtaccaata caggagcctg
    17821 tgaggcagtt tgtccgaaag gaatttctat tgaaaatatt gcccgcctga acagggagta
    17881 tatgatggca gggctggcca gcgacaaata agttgttttt aaactattta taagcccttt
    17941 atcacaaagg gcttttttgt tttcggccac tattcgtaca ttcagattct ataaaaaact
    18001 tttatgtaca caaaatctat ttcaattgcg gtagtggcta tttttttact tgtttcctgt
    18061 aaaaataatg atactgtgaa aaaagaatca gtaaaatggc ctgaaggagt aactgctcct
    18121 gtagccgaaa agaaaattaa agaattgaca gcgcatggtg atacccgtat cgatgagtac
    18181 tactggatga atgattattt taagaaaggt actgacagta ctaaagttgt ggattacctg
    18241 aaagcagaga atgcgtacct ggatacaatg atgtctggta ccaaaaaatt ccaggaggaa
    18301 ttatttgcgg aaatgaaagg ccgtatcaaa gaaaaagatg agtcggtgcc tgtgctgaac
    18361 aatggttatt attattattc ccgtactgag gaggggaaac agtattataa atattgccgc
    18421 aaaaaaggaa gcctggatgc accggaagag attttactgg acgccgataa aatggctgag
    18481 ggggcaggct atttttctgc gacaggtttc agtattagtc ctgataataa gttaatggca
    18541 tatggtattg ataaattatc acgccgccag tataccattt atattaagaa cctggaaact
    18601 ggagagttgt taaaagatga aataaaaggc acgggtggca atgcagtttg ggctgcggat
    18661 aataaaaccc ttttctatac ttctaataac ccggctactt tattatcaga gaagatcagg
    18721 aaacacaaat taggttcgga tgccaacact gatgtaacag tgtatgaaga aaaagatcct
    18781 tcgaactata ttggtgttta caaaacaaag tcaagggact atatagtgat atattccagt
    18841 gctacactat ccagtgaata ccggatagta aaagccagtg agcctgatgc ctctttcagc
    18901 ctatttcagc caagaataaa agatgtgtta tatgggattg accattgggg agataaattc
    18961 ctcatccgta ccaacctgga tgccaaaaat ttccggttaa tggaaactcc tgttgataaa
    19021 acaacccggg agaactggaa ggaagtaata gcacacagga cagatgtttt gctggaagat
    19081 atcgatgtat tcaaaggtca ttgggtgatc actgaacgta aggaagggct gttgcaaatg
    19141 cgtatcaggg aaatcgcttc tggtaaagag cattatcttg actttggtga accggcttat
    19201 gctgcatatg ttggtgcaaa ccccgagttc aatacaacaa acctgcgtta caattatact
    19261 tcgcttacta ctcccaattc agtttttgat tatgatatga atgcaaaaaa taaaagaatg
    19321 atgaaagagc aggaagtatt gggtggtttt aataaggctg attatgtgac cgaaagggtg
    19381 tatgcaactg caaaagatgg tgctaaagtt cccgtttcaa tagtatataa aaagggtaca
    19441 aagaaagatg ggtcagcccc tttattgtta tatgcctatg gttcttatgg cgccagtatg
    19501 gatgcaagct tcagcagcaa tcgtttaagc ttgttgaatc gtggatttgt atatgcaatt
    19561 gctcatatcc gtgggggaca ggaaatgggg cggcagtggt atgaggatgg taagttgatg
    19621 aagaaaaaaa atacatttac tgatttcatt gattgtggtg aatttctgat caaagagaaa
    19681 tatacttcta aaggtcattt gtatgcgatg ggaggcagtg ccggtggctt actgatgggt
    19741 gctgttgtaa atatggcccc ggatctttgg aatggagtag tggcgcaggt gcctttcgtg
    19801 gatgtgatca ccaccatgtc agatccaact attccattaa caacaaatga gtatgatgaa
    19861 tggggtaatc cggctgataa ggatgcctat ttctacatga aatcctattc tccgtatgat
    19921 aatttagaaa agaaaaatta ccctaatatg ctggtgacaa ccggactgca cgatagccag
    19981 gtacagtatt ttgaaccggc caagtgggta gcaaggctga gggttttaaa gactgataaa
    20041 aacctgcttt tactcaaaac aaatatggaa gccgggcatg gcggggcatc cggccgtttt
    20101 gattatttaa aagagattgc catggaatat gcattcttaa tggcactgga aggtataacc
    20161 agctaaaagg ccattgcagc tcgcaatgaa cctgaccaga tggcatcact cccggtttct
    20221 ttcatcagga ttaaacccgg agtgattgct attttagggg agatataata attacactgc
    20281 agagaggccc ctgtgttgaa ttgtacattg ctgctgtata tacctgctgc agggccagct
    20341 gtcaggcaaa aatgtatttt cttacccgca ttatatacgg cgccggcaaa tgcgtataaa
    20401 aaaatataag ctggatattt ataggaccca gctccgatag attctttttt gccaaagtaa
    20461 tatgcaacgc cactgtttgc ggtaaaccca attttttttg ctggcattga attaagtcgt
    20521 ccaaaacgat ttttgctcca ggcatataca gtactaaaac ctccgtaatg agtgttagaa
    20581 aaatcaccca gcgggatatt aattcctgct gcaattacac ttttttttcc accgggattt
    20641 attttttttt gaaaacctgt ctgcgagaaa caaaatgaag caggaagtaa aagcagaaat
    20701 aaggcaaaac tctttttcat aataagggtg tgaatccagg ccggcatatt acctgatcaa
    20761 tacaaaagta ccttttcttt ctatagtttc tcccgacttg ccaatagctt tgataaacca
    20821 gacataaaca ccggcttgct gaagcatgcc ttttactttt ccattccatc ctgcattacg
    20881 aaggctggtt gaaaaaacct gttgtcccca gcgactatag atgctgaatt ctttaagtat
    20941 tacctggcca tgataaaatg gtttcagtat atcgtttttc ccgtcatcat taggtgtaaa
    21001 tccagttgga atataaatat catcatactg taaaactgtg atcctaactg aatcttctcc
    21061 tttacagccc tcattgttac tggcagtaac tttataaaca atatcctcca gcggagtggc
    21121 aacaggattt ctaatgttac tattgcttaa ccatgtgtct ggtgaccata ggaaactatc
    21181 agcaggttca ttggttgaaa ggataacaga ttgtccaact gtgattgtct gatcgggtgg
    21241 tgcggtaact gaaaaattct taatttctac ctgctttatt aatgaatcat caatacaggc
    21301 agcaccagta agtagtttca gttttacgga ataaaaacct tcccggttat atatgtgaat
    21361 cgggtttgca gctgttgaag tattgccatc accaaaatcc catagatatt gtcctcctcc
    21421 attctgttca aaaagacttt tattgacgaa acggatataa ccggaatcac acctgactac
    21481 ataatcaaaa tcagcatcgg taattgtacc agttggtttt atgattctgc tgctttttat
    21541 gttaccacaa ctgacagagg aagaaatggt cagggaaaca gtataggatt gatttgccgg
    21601 agcaaatgta tgaaccgggt tttgcagggt tgatgtattt ccatcgccaa aattccagga
    21661 ccaggtgaga gttccgggca tgccagatat tgcagtaaac tgaactcttc cgctgcagct
    21721 gtcagttatt acatatgaaa aactattgtt gggatcatag gccacatcat taataaagga
    21781 gggtgaaccg aaacgggcag aaaatggtga aatatccaac tggttatcct ggaaattaca
    21841 acctgcacct ttaacagcag gagaattgat cacgccaaga ctttgacctg gtctgaatgt
    21901 ataaatcttt tcatttggcg ccagctgtag cgttaatacc ggcgaagtag ttgaaatgct
    21961 gatggttgat gcactaacag cagcggcgga aggcaaggta gcttccaact gatcgatctt
    22021 tctttcataa ggacgtacga gatataacat ctttgaatca gacgagtatt cgcaggcaac
    22081 atacattgca ttatcaaaca taacggacct tccgttagta acctggccgg ttgtattatc
    22141 aaagtcgaat agctgtacaa aattcggatt gcttacatca ggatcaaaca acgggaaatg
    22201 ggtctggcat aattgctttc cgtccgggct tatcttcata tagcctgtat tggtaagcag
    22261 gtgttgagta agtattacac cagcctgtga aaccacaggg tttgattgca gtccgttgca
    22321 ggtgatcagc caggaacgga aaatattaga attattatca ttggtgatca gccatatatc
    22381 cgtaccattc tgatgccggg cggcagtaag tctttctgta caggattgcc atagtagtgt
    22441 gtttttggaa accacttcgc cattgccatt atcccgtttc atatttacga tagaataatt
    22501 gtacccgctg ctgaaattat tttcaatggc atcagtggta aagatgtaat agatactgtc
    22561 atttccggga acgggaacaa taatagcagc ctgacaggat gaaatatttc ctttcagatc
    22621 atttccattc agcataacct ggtgctggcg attaaaaacg gttatcccat tcgtatagaa
    22681 tagcaattgc ccgttgctgt cagaaattgc agcggatcct tcatccgtga tcattgctcc
    22741 atctgtaagg gtaacaggtg ttacctgacc gggagaaccc gaaaagcgga tgcctgcccg
    22801 ctgaccaaaa taccagttat tgttctgttg tgcctgcaat gcaaaacaga taaaaaaatg
    22861 ggataaaaat ataattgtac tttttctcaa accaaacagg tatttcaacg tatttatgag
    22921 acccgcaaca acaaatttgc tgctttttaa ggggaattaa cagcatggga taactttcac
    22981 acaataaaac cagcgaattt tgcgattttt agctaaactc actgctattg aatcaattat
    23041 gcaggatatt tcttgttatt accctggcat tgacttccct ctggagcagt gcacaaattc
    23101 ctgtagccgg taaccctgtt tctaacctcc ggaaaaagac tttcaggata gatgcagaca
    23161 gcattaaagt agatacgatc agtatagttc ccaatacttt tttcatcagc aatgtggttg
    23221 ttgaagatta ccgcattgat tatgttaagg cgatacttta ctggaaaaag aaaccaattg
    23281 ctgacactgt caccattaca taccgggtgt tcccctttaa actaaatcct gtagcccagc
    23341 gtatgaatta cgatagtgtg atgaataatt tttatcagaa gccctttgaa tttaacaatg
    23401 gtttagccgg caatcaaaga ggagtctttg attttggaac attaaaagct gaagggagcc
    23461 ttggcaggca aattggattt ggtaacagcc aggatgcagt ggttaactct tcacttaatc
    23521 tgcagctcag cggtatgctg ggtgatagta ttgaaataca ggcagctatt actgataata
    23581 atattcctat tcaacctgat ggaaccacac aacaattaaa tgaatttgac caggtatttc
    23641 tccagtttaa gaaaagagga tggcagctga atcttgggga tattgatatc cgtcaaaatc
    23701 aaaattattt tttgaatttc tacaaaagat tgcagggtat ctcttttaaa acagagaatc
    23761 gcctgtcaaa aagtattagt tccaaaaccc tggtgagcgg ttctattgcc aaagggaaat
    23821 ttaaccggca gatactggat cctttggagg ggaaccaggg accttacagg ttgcggggac
    23881 cgaataatga attatttttt gttgtattgg ctaatacgga aagagtattt tatgatggac
    23941 aactgttaca gcggggagaa gaccaggatt atgtgatcaa ttataattct gcggaaataa
    24001 cttttactcc ccgcagaatg ataacaaagg atagcaggat acaggtggag tttgaatatg
    24061 cagaacggaa ttatttgaat actaacctgt atttatcgca ggagtttgaa atcaatcaga
    24121 aactgaagat ccggttaggg gctttcaata ataatgatgc taaaaactca cccattaacc
    24181 aggtgctgga taccaggcaa aaacaattcc tggctgacct tggtgatagc actcaaaatg
    24241 cgttttatcc cacagcgtta attgattctt ttgctgccga taaaatactt tatgagaaaa
    24301 tatatgtggg attggattct ttttaccaat attccacaaa tcctgcgacg gctaagttca
    24361 gtctgtcttt tgtggatgta aagcagggaa atggtaatta tgtacctgaa tttaacgggg
    24421 ctaatggaaa agtatacaaa tacctgccac ctgttgctgg tatcaaacaa ggacaatatg
    24481 aacctgtgca aatattggtt gcacccaggc ggcaacaaat agtaaatctg ggaattgatt
    24541 atgctattga taaaaatacg ttggtaaaag cagaagtggc aaccagcaat aatgatgtga
    24601 atactttctc cacgaaacag gatggggatg acaggggatg ggcgacaaaa gttcagctga
    24661 gtaataaaaa gatgctgagg gctgcaaaaa aactggaatt gacaaccggg ttggattatg
    24721 aatatgtgca aagtaaattt aaacctttgg aaagattacg taacgtggag tttacaagag
    24781 attggggcct tactcttttc tctgttccag ttaatgagaa tatcatcagg gtgtcagcag
    24841 gtctgaagga taagaataat catgcagttt cctaccagtt tatcaattat aaccgcagtg
    24901 ataattataa tggatttcag aatgtaataa aacataatgc tgactggaaa ggctggcggt
    24961 tcaacaacca gtttactgtt acaaatttta atgtgcaaaa tgataaagga actttcttac
    25021 ggccaaccct tgatttaagt aaacagttga agaaaatgga caactggaga gtggggctta
    25081 attatgcgct ggagcaaagt acttcccgtt ataagacaaa tgatacattg actcctaccg
    25141 cattttcatt tgatatctac tctgcttatt taaaatcgga tgaaagtaaa aagaataaat
    25201 atggcattac cttctttaca aggtctgata aatacccggt cggcaaagaa tttgtgagag
    25261 gtgaccggag cctgaacctg aacctgcaaa cagaattact cgcaaaccct aaacgccagt
    25321 tttacctgaa tacgacattc cgtaaattaa aagtgtacaa tgccactttg tccagacaaa
    25381 aagaagatga aactatttta ggacgggcag agtatgtaat gaatgaatgg aaagggttac
    25441 taacgggaac tgttttatat gaagtagggg cagggcagga acagcgcagg gattttgcat
    25501 atttagaagt gcctgccggg cagggtgaat atgcgtggat cgattataac aatgatggta
    25561 ttcagcaact gaacgaattt gaacttgccg cttttcctga ccaggcaaaa tttatacggg
    25621 tgtttacacc aaccaacgat tttataaagg caaattatat cacattcaac tacaggcttg
    25681 atataagtcc aagggctgta ttaaactcac ctaacctgaa aggtgcgaag aaactattgt
    25741 caaaattgaa cctgctcacc tcatttcaga ccagtaaaaa atcggttgcc agcggaggtt
    25801 tcgaattcaa tcccttcaaa tatggggtaa atgatacggc attaattacc ttaagtacta
    25861 ccatcctgaa tacactttcc tttaaccggt ttagcagtaa atggggaatt gatttcagca
    25921 attatcgtaa taacgggaaa tcattactta cttatgggta cgagagcagg aaaataaatg
    25981 actggtctgt aaaatggcga tggaatatta gcagaagcct ggcgcttact gtaaatggta
    26041 aaataggaac aaacgcatta tatacacctc agtttaccaa ccgcaattac cagctttcgg
    26101 tttacactgt tgaaccttca gtgatttata taaaaggcac aaagttcagg atagtgagca
    26161 gctacaagtt tgatagtaag aaaaacctgc ccctttacgg gaatgagaaa tctatctcca
    26221 attcattgaa tcttgaatca aggtataata tcctgcaacg gagctctctt accggcaagt
    26281 ttacttttaa taatattaat tatgattctc cggccaatac aacagtaagc tatattatgc
    26341 tggatggtct gttgccggga aaaaatttcc tttggtcact gggctttaac aaacggctga
    26401 ttaataacct ggagttgaat tttcaatacg atggtcgtaa agccggatca tcccgtactg
    26461 tacatttagg aagggcggga gtgacggcat tgttctaagt cgccagcttc ctaaagggac
    26521 ttgatttttg atctatataa aattaaaggt ggccagcgta ctatacattg aaccggaaat
    26581 gcatcacatc cccatctttc accacatact ctttgccttc aattcttaat ttaccaactt
    26641 ccctgcaggc tgcttccgaa ccatactgaa caaaatcatc atagctgatc acttctgcct
    26701 tgatgaaacc tttctcaaaa tctgtatgga tcacactggc tgcctgcgga gctttccaac
    26761 cctggtgaat ggtccaggcc ctgacttcct gcacacccgc agtaaagtaa gtgtagagat
    26821 tgagtaattt ataagctgag tgaatcaacc ggtccagggc aggttcttcc atatggtatt
    26881 cttccatgaa caattgttta tcctccccat tttccatttc agcgatctgt gcttcaatgt
    26941 tattgttcat aatgatcatt tgcgccttct cattttttat cgattccttc aaggcttccg
    27001 aaaatttatt cccggtgtgc atggatggtt catcaacatt ggcaacatat aacacaggtt
    27061 tgtcggtcaa taagcataga tcggcaatag catttttttc ttccttatct aaatccagtt
    27121 cccggatact tttccccttt tcaagatgtt ctttacaccg gatcagtact tccagttctg
    27181 ctttatattt aggatccagt tttatttgct tttctgtttt ctgaattttc ttctctacac
    27241 tatcaaggtc ttttaactgc agttcggtgt caatgatctc tttatcgctg acaggattga
    27301 tggctccttc atctcttaat atattttcat cttcaaaaca acggaccaca tgaatgatgg
    27361 catctacttc ccggatattg gccaaaaact tattacccaa tccttcacct ttgctggcac
    27421 cccgaacaag gccggcgatg tctacgattt cgatctgggt aggtacaata cggtcgggca
    27481 ctaccagttc tgccagttta tttaaacggg ggtccggaac atcaaccagg cctacattag
    27541 gatcaatggt acagaaacgg taattgcttg cctgtgcttt ggcactattg ctaaccgcat
    27601 taaataaagt tgattttcct acattgggta atcccacaat tcctgcttgt aatgccatat
    27661 cttttatttt tcgaggcgca aagataaggg ttaacagcct gatggaggat tgaaatcaaa
    27721 cctttaactt caaagcctaa acatgaattc acatggcatt gagcagaatc tggacagctt
    27781 tcattatcat agccttgctg gtagcggctg gaaaatttgt ctttgaaccc gggcagcagc
    27841 atattttcag cagcctggtt acaggaaaga atacagacac cattgtggct agaactttgg
    27901 attctgcagg tatccctgct tcggttcaca gccagctgat cgatcagaaa gtagcgatat
    27961 ggggtaaaga aaaagtgtac aggattagtg atggaggttt cagggcttac cggttattgc
    28021 atgcgaacgg aatatttgaa actacaaaag atgctgtcaa tctttgtatt ggtctgattg
    28081 gtatcatggc attgttcatg ggatttatga atattgcaga gaaggcagga ggtatccggt
    28141 tcttgtcgag gataatcggg ccattctttt cccgcatatt cccggatgta ccaaagaacc
    28201 atccttccat gggacatatg gtgatgaatt tttctgcaaa cctgatgggc cttgataatg
    28261 cggctacacc ttttggtata aaagcgatgg aaagcctgca ggagatcaac cctgataaaa
    28321 caaccgcctc taacccacag ttaatgtttc tttgtttgca tgcatcaggc ttaacattga
    28381 taccaacaat aattatcgct gacagggtag ccttaaaatc acagcatccg acagatattt
    28441 ttattccttg tatgattgcc acttttgcag ccaccatcgc tgctatgtta atagtggctt
    28501 accggcaacg tatcaggata tttcagtcgg tcatcctgct atgggtattg ggtatctctg
    28561 ccgttatcgc tgccctgatc ttatatatca aaacactgga tgcaaaaggg gtacaattat
    28621 tttctaccag tctcagtaat ggtattatcc tgttgatctt tttactgatc atactgggtg
    28681 ctgtttacaa gaagacgccg gtatttgatg cttttataga aggcgcaaaa ggaggttttg
    28741 aaacagctat ccggatcatt ccctacctgg taggtttatt agtagcgata agtttgctga
    28801 gaaacagcgg ggcttttgaa tttgttatgg atggcattaa aaatctgttc attttcttcg
    28861 gtggcgatat acgggtagtg gatgcactgc caactgccat tttaaaacca ttgagcggta
    28921 gcggggcaag ggctatgatg atcgatacgt tgaatgcaca tggggtcgat tcatttgcgg
    28981 gtaaccttgc ctctgtcttc aggggctctg cggatactac tttttatatt atcgcagttt
    29041 actttggtgc agtaggaata aaaaataccc gctatgccgt aacgacaatg ctgttggcag
    29101 accttgctgg tattattaca gccattgcaa tggcttattt attctttgga taaaaaaaag
    29161 gctgttcatc atgaacagcc ttcccgacgt ttattttttt attctttagc ggttaatgaa
    29221 agacgataat gtcctggcaa tagtaccgga tgtagtttca tatcttcctc tgaatactaa
    29281 agtatagctt cttttttcag tgggggttaa accgttcaaa acagccagtt gattggtagt
    29341 ccctgtttct cttacataaa aggtatcagt aaatgcagaa ggcacaggaa taaaatctgt
    29401 tactccggta actgccacat tggtaaatac atttacattt cttctttttg agaagatgtc
    29461 aacagccgga acagcatttt ttgagaaaat aaaattggca aaccgaagtc ttgcagtagt
    29521 atctccgggt acaacaattt tggtttccac tgttttctgc tttgcagcat tgatggtgtc
    29581 gtacatgaaa atggtataat ttttacctac atccatattt tgtgcaaaag acagtggtgt
    29641 ttgtgtggtg gctgttaaag tatccctgat catgaaagcc ctgtatccac cggggacagc
    29701 aaaagcaaaa ccggtagagg ggaaaacact accgagtatt agctttgcgc catttacagg
    29761 tttagaatca acatatacat aattacgatc cgcatttact gttgccatat atacctgcag
    29821 caagctgcta ttgctgaaat cccggttctg ttccactttt tcatcaaagg atttagtaca
    29881 tgatgaaaac atgaatgttg cagccaggct ggcaacccca agaatagttt tatttatcaa
    29941 cttcataagt tttcagtttg tgagttagtt attaaggttg agagaaccat tgttctttgg
    30001 tatgatagtc cagtgctatc gccccgatac ttgttagtgc tgcaacatta tataaatatt
    30061 cagaattata ccttggacgg gcacggtaag ccaatttccc attgttatca ggatacaaat
    30121 ctgatccact tggagcggca aaatctgcat atacctgcat accggtaaca ggatccaggt
    30181 ctgtataatg atatcttctc atatctaccc atgtttgcat gaatccataa ccatacaagg
    30241 ctatgtattt ctgcatcatg atatgtgata aggtaagatt ggcggcagtt ggaaccacgg
    30301 ccgggttggc caggtaagct gctcttgtag ccggtgtgat ctgcatggca gcaggcactt
    30361 tattatgaaa ttctggtgtg ccggtaagaa aatcaaaact caggttaagg ccatcgatat
    30421 aagctgtaag ggcgcctggt ttgttgccag tcaaccacaa tgcttctgct ttcataaaag
    30481 ctatttcagc agccgtgata acgggaaatt gaactccatt ttggaaaata tacctgcagg
    30541 ggttatcgtt tccgggagct gcagttaaag caaaactact accccagaaa ttttgcggta
    30601 gatcagctgt agccagtcca tctggacctt ttgtaggacg aaccccttta taagtgccat
    30661 ttgtgttttc ccggatgata taaggagccc ttggatcagc tacccctaaa aacatggaat
    30721 taacaccaga catcaggtcg gcaataaaac gggtctggcg caggacaccc acattgcccc
    30781 tgataggtcc gaagaaattg gatgtaccgg tgataccagt gttcgcaaac tttgccgctg
    30841 cattatctgc attactgtta atggcaaggt tacaatagta taaaactgaa tcagcctggt
    30901 agttggctcc tttatttgtt aagtgaaagg aacgggccag tacagagtaa acaaattttt
    30961 tccatttgtt tacatcacca ttataaaagt aagcatcccc aagggcaaga tttgcctggc
    31021 ttactccgcc acctgtttta tttaggtttt caattgccag gtgggcttgt cttctgactt
    31081 cggcataaat atcctcctgt gtgtcatagt caaaaacaag acgattgggg tcaaaagcct
    31141 gcctgagtat tgcttcaccg tacatattgg tcagtgtcag ccagctccat gccctgatgg
    31201 catggcccac accaacatag tcccattttt cttcttcggc agcccagtca atcattcttc
    31261 ccaggttttg tcccattcca taataatgca tggcccaaac agaacccagt acatcactgc
    31321 cgccagtggc gccactcata cggtcgtact ggttcaatgg tgtatttgtt gcccagaact
    31381 gaacataccg gccaacatag agaccgtcgt tagcgagacc ataggcagat ccctgtgctg
    31441 aggaacttcc gatgaaatta cctatgattc cgggtaataa agtttcaaca ggcacacggg
    31501 tagtggcatt gggatttaaa aatgcttcgt caattttttt actgcaggag ctgaacagca
    31561 gtacggctgc taccggcagt aagcaaaatt ttattaatag tttattcatt tttcttaagt
    31621 tttaaaggtt agaaggatgc cctgattcca aagtttacag taagagcagc gggcagtgta
    31681 ccataatcga aaccataagc acccacaccg cggctgccgg atgtattacc attcaccatg
    31741 gggtctgcac cactataatt agtcaatagt accaggtcgt tgccggtaac aaaggcgcta
    31801 aggcttttta tccctttaat aaaattggat gaaaaggtgt aactaaaaga aatatctctc
    31861 aacctgaacc agttcacatc tttttcaatg aatgcttctt caggcatggt agtataatag
    31921 tcctgctggt aataaggagt aatagcaatg gtattaggag taggagtggc tgaattctgc
    31981 agcccgtcat ttaatactcc ctgtatcacc cttggaagca gcctgtcagc agtcaatttg
    32041 ctgcggcctg tacgtgtcag gaacatattg tttccgttga aaatatcgcc acccactttc
    32101 aggtcccaca gcatgctgag tttccagttc ttccagctga aactattcag ccaacctaac
    32161 gtgaaattgg ggttcctgtc acctcttact ttaaaaagac cttcaacaac cggtaagcca
    32221 ttcaacgggt tgatcagtat ctgcccggca ttattcctgg cataaccaaa agatgtaata
    32281 gtagtagtcg gtccgccaaa aacaagaccg cctcttgcat tgccatacag ccatgtatca
    32341 gctatgtagt attctgcaac attacgtggc atatcttcta ccttattcca cattttgtta
    32401 aagtttaatc gcatattcca ttgaagagct ttcttctttt gtaacatgtc aaagttcaat
    32461 gctacttcaa aaccctggtt cctggttgat gctgcatttt gtgtatttaa tacgaaacct
    32521 gttgcgtagc tcaggcggaa tgcattaatg atctgacctt tattcagtgt gttataatag
    32581 gtcatatcca catttaaccg gctgctgaat aagcggaact cggtacccaa ctcataagtt
    32641 ttttgttttt caggagccag gtctgcatta ttattaaaga aaccgtaaga gaaaccacca
    32701 ccactggcaa aattgttcac aaatacagat tgagtggaat agggtggatt cagtcttgcc
    32761 gttccggcca aagaagttct cagtttccag taactaagca cactgttctt tttcaaagca
    32821 gggaagatat cactcacgat caaacttaag cttccacccg ggtaatcata tttcctgttt
    32881 ttttcaggta gggtagaggc ttcttcaaac cggtgtgtat aagagaagaa ggcatagttt
    32941 ttatagctta cagcaaattc tccaaatact gcaaactggc gggaaatact ttgattgtac
    33001 ctgcctaaat tattttgcaa taaacgcagc cttgtattag tccgggtaat gctgctatcc
    33061 atataatcac caacaacccc cctgagatca ttatctgtta atatagtgcc attcttatac
    33121 atctttccag ttactcctcc aacagaatca acaatattgg taccgtagat agcaaacatt
    33181 tttgtttcat aatcctgcca catggttcca cccattaaac gcagttcgag gtcttttcct
    33241 atttttttct ttaccgttgc tgtaatggta tgattatagc caatatattt tctccagtaa
    33301 ttatctaatg ttccgccggt tccccttgca agataaaagg actgcggatg gtagaactgg
    33361 taaccaacca tgtcatacgt atcatatcca aatcttccgg ttaaagacaa ccaggagaaa
    33421 ggattgaaat ttacgcctaa tgaatacgtt ttccggtccg tttcatcgta agacctgttt
    33481 ttctttacat tgaaaagcgg gttgtcgaaa tcagcattgg ggtttgaatt aaacaaggcg
    33541 agcttatctc cattgggatc ttcgaaagat ctgatatcat tggttgaagg ccagataagc
    33601 aaactcaata agtaaccacc ggcacttctc agcactttgt tgttatcaga ccttgtgtaa
    33661 gcgaatgagg gagacagttc cagttttccc ttaaacagtt ttgtggtatt agagatacga
    33721 aaagtgtagc gtttgaaatc attttcaggc acaacgcctt tttgatcaaa gtaactgccg
    33781 gatacacgga attttgattc tttaaaacca aagtctgcac ccaggttatg tgtttgagaa
    33841 gtaccgttcc tgaagaacgc atctttgttg tcaaataact gggtaccggg agcataggcc
    33901 ggtccaaaat atgtgaaaac attgctggga ataccattga taccattctc gtattcatcc
    33961 agtgtttcgg gccagcgggt cactttctga acacggaaac tgttatcata ctgaatgccc
    34021 agtttgtttg tttttgattt ccttgttgta attacaatag cacctgagct ggcctggctt
    34081 ccgtaaaggg cggtagcttc aggtcctttt aatatagtta tggtttcaat atcactaggg
    34141 ttaagatctg caatcctgtt ttgataatca gtattacggt taggcctgtc agaggctaaa
    34201 ccaatgtcct ggccaccacc ggatgtttcg ttgatagtgg aattatccat aatgatacca
    34261 tcgatcacat acagcggctg gttactcagc gataatgaat tgtaaccacg cagtacaaca
    34321 gaagttgatg caccagctac accagatgta ggatccatag taagacccgc aacccggcca
    34381 cccaaactat tcaagaaatt ctctctttgt gtttcctgta tttctgcacc atccactttt
    34441 tgtacagagt agcccaacga ccttggattt cttttgatat ccatcgctgt tacgactact
    34501 tcacccatca tgttgtcggc tgtttttaaa ctgatgccaa cagtttcacc agaacctggt
    34561 ttaacatctg ttgttacata gccaacataa gagacctgga gtgtttcacc cgggttcatt
    34621 ttgagggtaa acttaccgtt cgcatctgtt tgtgtaatgc ggcgggtacc cttcacccta
    34681 acggtaacac cagccagtgg tgtcttacta tcctctgaca aaatggtgcc ggtgatagtt
    34741 cgctcttgtg cctgcacaga gggcggcagc aatagcaaca tcagcgataa tgccaggaat
    34801 acacggagaa tttttctcat agctgttttt ttttgatata attattaata agcagttcca
    34861 aaatttatta caacactttt aatccggcat ccttatatgg tcttaatacc gggtcgtttt
    34921 catttaattc agttatcaat atatcaattt catcaatacc acaaacacgg ataggctgaa
    34981 ctgtatttat tttttcggcg atactcaggc ataccacctt tttagaagac tctatcatgg
    35041 ccttctttag ctgcacgacc tcccaatcat tatctgtgat gccatgtttc acatcaatgg
    35101 cattggtacc caggaaacaa aggtcgggtt tcatctgttt tattttggca attgcctcag
    35161 aaccaacagt tattttagag ttttttgaaa ttttatctcc gattaaaatt acctcaatat
    35221 tggggtgata catgtattcc aatatcaccg ggatacttcc ggaaataaaa gtagctttta
    35281 actggggagg caatgaacgg gccagttcca tgatggttgt gccaccgctg gttaatacaa
    35341 acatgccatt attaatgagg ctgatggctt tttgggcaat cgtctttttc tggttttggg
    35401 aataaactcc gtttaacgga agagacacat cattgaaaga atgggataat gcaccgccat
    35461 gaacttttat tacttttccc tcttctgcca gttcctggag gtcacgacga atagtatctt
    35521 ctgacacctt tatttcggtg cagagagaag aagagagaac cttattatga aggttcacct
    35581 ggtgcaggat atatgcctgc ctctcttttt taagcattcg tcagttttga ttcactaaaa
    35641 tagtaaattc ctgcataaaa acgcagaaac cggataatat ttgcaatatg tggaaaaatg
    35701 ggggaaagtc aatgctgcca tttattttat caatcgcagt atgaccaatg ataaagaagc
    35761 tgtttcaaaa gccccaatca ttgtcatatt gagcaaagcc gaaatagggc aatgattgg
//
LOCUS       DAORTI010000018        35560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_748_length_35560_cov_8.604112, whole genome shotgun sequence.
ACCESSION   DAORTI010000018 DAORTI010000000
VERSION     DAORTI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 35560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35560
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_35560_cov_8.604112"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLO70_04255"
     CDS             <1..949
                     /locus_tag="PLO70_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HQZ73699.1"
                     /translation="KIIRPHHQILSLLMNSLLKIHPEVQEALTNNKPVVALESTIISH
                     GMPYPKNVETALAVEEVVRANGAVPATIAIFNGKCHVGLTNEQLEYFGKAKDVWKVSL
                     RDMPYVISQNLYGATTVAATMRIAAMAGIKIFVTGGIGGVHRGAETSMDISADLTEMA
                     QTSVAVVSAGVKSILDIGLTLEYLETKGIPVITYGQDEFPSFYSRKSGFTSPLRLDTT
                     AEIALLLDTKWKLGLTGSVLIANPVPAEQEMATDEMELHIQKALNAAKEKNISGKNVT
                     PFILQYIAEHTKGESLETNIALIKHNAKVGAEIAVEHAG"
     gene            complement(985..1057)
                     /locus_tag="PLO70_04260"
     tRNA            complement(985..1057)
                     /locus_tag="PLO70_04260"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1022..1024),aa:Phe,seq:gaa)
     gene            complement(1176..3437)
                     /gene="recQ"
                     /locus_tag="PLO70_04265"
     CDS             complement(1176..3437)
                     /gene="recQ"
                     /locus_tag="PLO70_04265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HQZ73700.1"
                     /translation="MGTTAKKATVKKTAKVKEAAPSKKRFDLHKALQEYFGFDKFKGT
                     QEKAIESLLAGHDTFVIMPTGGGKSLCYQLPAMVSEGVALIVSPLIALMKNQVDLVRG
                     YSSNDEVAHFLNSTLNKKEIKEVHDDLLSGKTKMLYVAPETLTKQENLEFFSDLKISF
                     FAVDEAHCISEWGHDFRPEYRRLREMMIQINPDAPVIALTATATPKVQSDIVKNLDLR
                     KPNIFISSFNRANLYYEVLPKIKKAQTDENIVRFIKGMKNKSGIIYTLNRKTTEELAD
                     ILNANGIKAVAYHAGLDSKLRAERQDLFLGEEVQVICATIAFGMGIDKPDIRFVIHYN
                     IPKSIENYYQETGRAGRDGLEGKCILHYSHKDVSKLEHLMRDKPLSEREVGAQLINET
                     VAFAETGVCRRKILMSYFGEEYEKENCGQCDNCKHPKERVEAKEEAAIALKTIKALDE
                     RFATDYVVQIIIGRMTPQNQMFRHDGLEVFASGNDKDSHFWNSLIRQLILEGLLTKDI
                     EEYGVLKFTKKGEAFLKKPKSFQIVLNKLYDDANADDEEGTEATGGAAMDEKLMAMLL
                     ELRQKEAKKKNLPPFVIFLESSLQDMATFYPITMEGLEKCQGVSKGKAIKYGKPFVEL
                     IARYVEENDIERPDDFVMKSVVNKSGSKVYIIQNTDKKVSLETIAKNKGWRMDEMLEE
                     METIAASGTKLNLDYAIDEMLDEDDQDEIIEYFKSCETSSLQVAQEELAEYNFNWEQL
                     KIMRIKFLSEYGM"
     gene            3818..4780
                     /locus_tag="PLO70_04270"
     CDS             3818..4780
                     /locus_tag="PLO70_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQZ73701.1"
                     /translation="MAVSIKQVALKTIALEANSVSGLASYINDDFEKAVTAIASGKGR
                     VVVSGIGKSAVIAQKIVATFNSTGTPSIFMHAADAIHGDLGMVQQNDVVVIISKSGES
                     PEIKVLVPLIKNFGNVLIAMVGNIESYLARNASIILNTTVTQEACPNNLAPTSSTTAQ
                     LVMGDALAICLMELNGFQSEDFAKFHPGGSLGKKLYLRVVDLYADNEKPKVLAEQSLK
                     EVIVEMTAKRLGITAVVDTSNNLLGIITDGDLRRMLEKSIAIDTIKAGDIMTVNPKTI
                     GPDELAVAALDVLRKNEITQLAVTDAGKYLGIIHLHDLVKEGLI"
     assembly_gap    4813..4912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4913..5557
                     /locus_tag="PLO70_04275"
     CDS             <4913..5557
                     /locus_tag="PLO70_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQZ73702.1"
                     /translation="VKPTYPNTGYGYIQHDTAEAAPDVYKVKTFTEKPNVELAKAFIS
                     SGDFLWNAGIFTWKVKNVLTAFEKYLPEVYEVFAAEKDKFNTDKEKEAIESIYPQCTN
                     ISIDFGVMEKAENVYVIPATFSWSDLGTWNSAWENKEKDYFGNAVVGKKVMAVDARNC
                     MVHVPDNKLVVLQGLDEYIIVDTKDVLLICKKDKEQEIKEYVAEVKRNMGDKYL"
     gene            5643..6359
                     /locus_tag="PLO70_04280"
     CDS             5643..6359
                     /locus_tag="PLO70_04280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQZ73703.1"
                     /translation="MYPVASILFLDIETVPQYSSYNEQPEEWKTLWGLKAQYLIRNKE
                     EETVETIYPRAGIYAEFGKIVCISCGFIQGAGTDKKIILKSFAGDEEKKLLYEFSEML
                     KKWSADGSKFLCAHNGKEFDFPYLCRRMVINQIAIPSILNMSGKKPWEVNHLDTMDLW
                     KFGDFKNYTSLNLLAHSLGIQTPKDDIDGSMVWEVYWKEKNLPRIVTYCQKDVVTVAQ
                     IFLRMNGEPLIREENIEIKP"
     gene            6359..6745
                     /locus_tag="PLO70_04285"
     CDS             6359..6745
                     /locus_tag="PLO70_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQZ73704.1"
                     /translation="MKRINYSSGAKWEDIVGYSRAVKVGNIIEVTGTVAVDENSSLVG
                     KDDAYAQTKFILEKIESVLEKAGASLKDVVRTRMFVTDISRWEEYGKAHGEFFATIKP
                     CTSMIEVKGLIAPEYLIEIEATAIIS"
     gene            6747..7073
                     /locus_tag="PLO70_04290"
     CDS             6747..7073
                     /locus_tag="PLO70_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQZ73705.1"
                     /translation="MNIETLREYCLSKPGAEETLPFGPDTLVYKVGGKMFLLTGLDSE
                     DLRFNVKCDPDKAIEWREEFTCVQPGYHMNKKHWNTIVADGSVPDKQLKEWIDHSYEL
                     VAKKKK"
     gene            complement(7075..8046)
                     /locus_tag="PLO70_04295"
     CDS             complement(7075..8046)
                     /locus_tag="PLO70_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HQZ73706.1"
                     /translation="MLFNKVHAFFNPEQFQGWGKTKNYFEGWYFKVVNADATKAFAFI
                     PGIAMDAAGNKHSFIQVLDGKKQTAGYHRFTADDFYSLPKNFRVSIQNNSFSGSGIQL
                     SLPGLTGELQFTGNIPWPKPWYSPGIMGPYAFVPFMECYHGIVSMDHRVDGMIIVNGE
                     TIDFTNGRGYIEKDWGRSFPSAYFWMQSNHFSQPGISLKCSVAKIPWVRSSFVGFIAG
                     IWLHNRLIKFTTYNQSKLLTSFADEKKVKIVLENKQYRLEILAHRDTATELASPIRGF
                     MEGRIEESMTSAIEVLLTDNKTKEILLNDTGKNAGLEVAGKIKEIFV"
     gene            8136..>9275
                     /locus_tag="PLO70_04300"
     CDS             8136..>9275
                     /locus_tag="PLO70_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQZ73707.1"
                     /translation="MSIPHIHSKLPNVGTTIFTVMSGLATHHNAVNLGQGFPDFPMNE
                     ELTELVNWAMKNNFNQYSPMPGWFPLRETIAEKVEFLYHTRINPDTEITITPGGTYAI
                     YSAFTAILNPGDEVIVFEPAYDSYIPNIEINGAKAITINLVYPDYHIDWAAVKKAITS
                     KTKAIIINSPHNPTGSVITENDIQELRKIVEGTNIFIISDEVYEHLIFDTIPHQSILR
                     YPDLLQRSFVCFSFGKVYNCTGWKLGYCIAPPELTTEFRKVHQFNCFSCHTPSQVALA
                     QFLQNKEAYLSLSSFMQEKRDYFSKLMRGTKFEMLDSHGSYFICAKYDRISDEPDKEF
                     AIRLTKEAGVATIPVSAFYQNGKDDKVLRFCFSKKNETLEKAVEKL"
     assembly_gap    9276..9375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9376..10035
                     /locus_tag="PLO70_04305"
     CDS             <9376..10035
                     /locus_tag="PLO70_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQZ73708.1"
                     /translation="YTLLYCILHIPLGTAMTYNLTSALFIAVFSFLLFREYHGRVVLL
                     AVLLGFLGMLLIYKPAMHFPWYYHAAGLISGVASALAYITVNRLAGYYDSRIIVLAFI
                     GTGVLVPLVFMIIRYSADIPPDDVFFISWKWPSGFEWFYVLWLGLAALFGQYFVTKAY
                     GADKAGIVSSIGYANIIFSVFIGMALGDAFPDWMSSLGILCIISSGVLISVFKTKNKV
                     S"
     gene            10106..10441
                     /locus_tag="PLO70_04310"
     CDS             10106..10441
                     /locus_tag="PLO70_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQZ73709.1"
                     /translation="MAFHKKEEFTQKEQDLANFAKAMSHPARIAILKVLAQRNECICG
                     ELVDMLPLAQSTVSQHLKELKNAGLINGETDGPRSCYCINWKAFEKFNNEFNLLFSNL
                     KIKNEKACC"
     gene            10422..11303
                     /locus_tag="PLO70_04315"
     CDS             10422..11303
                     /locus_tag="PLO70_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HQZ73710.1"
                     /translation="MKKPVAELIYHLKIVTMNTDAQLKEIVRQKYSEIALQDKETNQS
                     SCCGAGGCSTEVYNIMSDDYTTLEGYNPDADLGLGCGLPTQFANIKKGDTVIDLGSGA
                     GNDCFVARAETGETGKVIGIDFTPAMIDKARANAEKRGFNNVEFRQGDIEKMPVTSNV
                     ADVIVSNCVLNLVPNKDGVFKEIFRVLKPGGHFSISDVVLVGNLPEALRKDAEMYAGC
                     VSGAIQKQVYLELIRHNGFQNITVQKEKPIIIPDDILKNYLDDEELNKFKNGTTGIFS
                     VTVFAQKPACTPGGGCC"
     gene            11473..12513
                     /gene="arsB"
                     /locus_tag="PLO70_04320"
     CDS             11473..12513
                     /gene="arsB"
                     /locus_tag="PLO70_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQZ73711.1"
                     /translation="MPKKLSFLDRYLTLWIFLAMGIGVAIGYFFPAAESFINSFQSGS
                     TNIPIAIGLILMMYPPLAKVKYEELPKVFANKKILLLSLVQNWIVGPVLMFALAVIFL
                     RDEPGYMTGLILIGIARCIAMVIVWNDLAGGDRLYAAGLVAFNSIFQVVFYSFYAWIF
                     ITKLPPLLGLKGYEVNITIGEVAQSVFIYLGIPFLAGMISRYVLIKWKGEDWFTKKYL
                     PAISPITLIALLFTIVVMFSLKGNMIVEIPGDVLLIAIPLTIYFAIMFFTAFYLSKKA
                     GADYEKSTSLAFTAAGNNFELGIAVAVSVFTIHSPVAFAAVIGPLIEVPVLLLLVRFA
                     LSQQKKFKPAKQ"
     gene            12510..12911
                     /locus_tag="PLO70_04325"
     CDS             12510..12911
                     /locus_tag="PLO70_04325"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQZ73712.1"
                     /translation="MKKKLLFVCIENSNRSQMSQAFAKILGGANVEAYSAGSKPSGIV
                     NPKAIAAMKELGYDLSAHDSKSLKEVEQYAPFDAVITMGCGDACPWMPAKKFIDWQIP
                     DPKHMEPGEFNKVRDLIKEKVAGLLEELEKI"
     gene            complement(12908..13444)
                     /locus_tag="PLO70_04330"
     CDS             complement(12908..13444)
                     /locus_tag="PLO70_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73713.1"
                     /translation="MKTVSYLIIITSFFCTISLFANNRPDSIPANPKDVESPDALVAA
                     LYNVISGPAGEKRNWDRMRTLFLPEGRLMATGKKSDGTMGKRIMTVEDYIATSGPFLE
                     KEGFFEREIGRRTEQFGSVVHVFSTYDSKRKLNDEKPFMRGINSIQLWNDGKRWWILT
                     VFWQAETPDNLIPEKYID"
     gene            13543..15195
                     /locus_tag="PLO70_04335"
     CDS             13543..15195
                     /locus_tag="PLO70_04335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQZ73714.1"
                     /translation="MNNALFSRNLYIFLFSLLGIVYITGLFVPLMDNDSAHHANIALR
                     MYLTGDYVNLVDYGKDYLDKPHLHFWLAAFSYKLFGVNTFAYKLPSLLFTIIGTYSTY
                     RLGKALYTHETGKLAALLTASAFAYILANNDVRMDAILTASIAIATWQLVEFIQHKKI
                     MNIAGAALGLALGFCTKGHIAVFVPAVAAFFYILYLKDWKLLLNWKWLLLIALFGLFI
                     SPVVYCYYLQYNLHPEKIVRGKDQINGVKFILFNQSVERFSGEMGSDAKNDYFFFLHS
                     FLWAFAPWSILTYIAIFGRLKNFLHRKEEWLTTGVFLVMLVVVSLSGFKLPHYLNIVF
                     PTCAVMAASLLLQKREEPMWVKNIFRLQLFISSLLLVLAAVINFWAFPVENHRVMILL
                     VLLLTVFFYFFKTTTLRRIQKTVAVSVTAMVLCFFLLNANFYPQLLRYQGGNELAAAT
                     KETVNPDDVYFWKENYSSSYNFSTSTLRKPFTDSICQEGKKIWLLYDIRNEEEIKQAG
                     YKIGEKYSTRDYEITKLDLKFVNPQKRDSQCTKMVIAEVSRL"
     gene            complement(15282..16292)
                     /gene="nadA"
                     /locus_tag="PLO70_04340"
     CDS             complement(15282..16292)
                     /gene="nadA"
                     /locus_tag="PLO70_04340"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQZ73715.1"
                     /translation="MNTDTLDTARAAIEKRGFLDIELDPTLDLFAEIEKLKKEKNALL
                     LAHYYQEPDIQDVADYIGDSLGLAQQAEKTKADMIVFAGVHFMAETAKILNPAKKVVI
                     PDLKAGCSLSDSCPPPLFKKFKEQHPDHVVVSYINCSAGIKALSDVICTSSNAKIVVE
                     SFQKEQKIIFAPDKNLGAYINKVTGRDMVLWNGACMVHEIFSLEKITKLKVRHPNAKL
                     IAHPECEDPVLRIADFIGSTTGLLKYTQTDPSQEYIVATETGILHQMMKASPGKTFIP
                     APPNNNCACNDCPHMKLNTLEKLYLCIKYELPEIIMEEELRLAAKKPIDRMLEISARA
                     GL"
     gene            16917..19085
                     /locus_tag="PLO70_04345"
     CDS             16917..19085
                     /locus_tag="PLO70_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQZ73716.1"
                     /translation="MKSLKHLLVAFFLFVFTIARADEGMWLPLFLQQLNEKQMKGMGM
                     KISAKDIYNINSGSLKDAIVSFGGFCTGEVISSKGLVLTNHHCGFDAIQNHSTLERNY
                     IRDGFWARNLSEEIPNQGLFVTFIVRIEDVTKQALNGVTKTMSESERQSAIDKNIAGL
                     RKDNKRETYQDNFVRPFFEGNQYFLFVTETYRDIRLVGAPPSAIGNFGKDTDNWMWPR
                     HTGDFSMFRIYAGKDNKPAPYSTDNVPYIPKRSLSISLDGVSEGDFTMVFGFPGRTTQ
                     YLPSPAVDQIMTVNDPAKIAIRDKALAVIDGFMRKDEQIKIQYAAKYASISNAWKKWQ
                     GEVLGLTSTNAVGKKKAYEADFQKRVNTNPLWKTAYGNLLGDMESHYATLKPYGYARD
                     YFTEIVSKIELFTIAAQLNALITAYEKDGENGFKQRLPVTKNALEGLYTEYSPVVDQQ
                     LFEVLMEMYVKDQDKKYVSPMLIEWLNGDMNGFKKLAAKVYDATPLDDSKATMKMLEK
                     PAAELVAELKNQQVIRLYQDLLKTYQAEVQSKLGEINSNINKLQRTYMQAQMEVMKEK
                     KFYPDANSTLRVTYGKVKGYNPRDAVKFDFYTYLDGVMDKYKPGDYEFDVPEKLRSLF
                     KSKDYGQYGSKGKMPVCFIAMNHTTGGNSGSPALDAYGNLIGLNFDRVWEGTMSDINY
                     DPSICRNIMVDIRYVLFIVDKYAGAGHLVKEMKLVHPKKK"
     gene            19220..21343
                     /locus_tag="PLO70_04350"
     CDS             19220..21343
                     /locus_tag="PLO70_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQZ73717.1"
                     /translation="MSIIQDIRDKYAKLTVVLIALALVGFILTDYFSGQARAGGGGAS
                     STIGMVNGQKINFDEFNPKVTQMEENMKAQGYPQNASLTQQAMEQAWAQEINRLLLTD
                     EFKNLGIEVGKKELGDILYGANAPQDIKQQFTDEKTGQFNAVLAKQNIDQVLKKGTPE
                     QKANISNYINQLAELRMSDKYLSLLSNSTNFPRWMIEKQNADNSQLAKISLVREVYSS
                     IADSTIKIEDSEIAAYINKNKSQYKQQESRSINYVTFSAAPSAADSAEVKNRLVTLKE
                     NFKTAENLEQYLAGEGANYYSGYISGKTIQIALKDSIFKTLVGEVYGPYLDGGSYALA
                     RVLGVRQMPDTATVRHILISTQSRDSATGYNLADSLKNAIAAGSGFDSLAAKFSDDPG
                     SKDKGGLYENVPSGQMVPTFNDFIFLNPVGAKGIVKTDFGFHYIEILSQKGNGAAYKI
                     AYLPKEIIASQETDNAALNKANEFAGDSRDLKAFDANFEKTLKPLGIQKGIATDITPS
                     SSDIRGLGVSRSFVRSIYEADKGDVLKPERIDENYVVAVVTEVFEEGTMSPARARVSV
                     EPALRNKKKAEILIKKIGTVTTLEAAATALGGKTIETADSIRINSALSSTLGYEPRVT
                     GAAFNPANKGKVVPAAIEGMNGVYVVRVENVTTTPVTDGDVNGQRAARYQQAKQSFSN
                     QYSPNNPLSILRNAATIKDKRSNRY"
     gene            21527..22033
                     /locus_tag="PLO70_04355"
     CDS             21527..22033
                     /locus_tag="PLO70_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HQZ73718.1"
                     /translation="MSEPFINKVAESGLITLDLEKWYPRGEAAVFDMKDYLFMGMILK
                     EKDFREALKQMDWSVYEGKNVAVTCSADAVIPVWAYMLVAAYLQPVAKEIVMGDEKEL
                     HKALFLKNLSSINLEEYTDKRIVIKGCGETPIADYVYMEITKLLRPVVKSIMYGEPCS
                     TVPVYKKK"
     gene            complement(22080..23189)
                     /gene="corA"
                     /locus_tag="PLO70_04360"
     CDS             complement(22080..23189)
                     /gene="corA"
                     /locus_tag="PLO70_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQZ73719.1"
                     /translation="MNVKLHPEKYLKYLYPPNLFGTRRTKEILSVNPTILPHREEADR
                     VVVTVYDYTAEQLEERKAESVDTVFHYKESTSITWINIDGIRKADVEALCTKYVIHPL
                     IIEDILSVDQRPKMDEVEGVLYCLLNMLYFNEEKKTVETEQISVVLGTNYVISFQEDP
                     ARDVFDPIRHRLKMTKSQLRQRTADYLLYAMIDLIVDSYFIVMERLGDQIEALEEEVV
                     RKSNKRSLARINQLRKELIVLKRNIAPVRDLVAGIIRSESELLEDRTTKYFKDVYDHI
                     IQAFDLSENYRDVMMSVQDLYISNVNLRLNEVMKVMTIVTCLLAPATVIGGVFGMNFE
                     VIPYSHHEWGFYFAISFMILVPLAMLVWFKKRGWF"
     gene            complement(23407..23694)
                     /locus_tag="PLO70_04365"
     CDS             complement(23407..23694)
                     /locus_tag="PLO70_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ73720.1"
                     /translation="MNIKDLHTDTKSVSTASLFKTAEGTVTSIHILAGEQLKEHVTKV
                     PALLVCLAGEALFENENGIKLTLTTGDYVNIEPNTRHWINAISTSNLILIK"
     gene            complement(23691..24083)
                     /locus_tag="PLO70_04370"
     CDS             complement(23691..24083)
                     /locus_tag="PLO70_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73721.1"
                     /translation="MKKIILYLVAAVLTGFGLLTLFLSASVIFDLFDIRSKEGNYVLF
                     VVWSNFISSLLYLVAVYGLITSKKWTYKILGLSAIILTIAFIGFLFYIQSGGIHETKT
                     VGAMVFRIALTVAFTLFAYFTINKKKKP"
     gene            complement(24083..24493)
                     /locus_tag="PLO70_04375"
     CDS             complement(24083..24493)
                     /locus_tag="PLO70_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73722.1"
                     /translation="MNGKNNIAIGFLTMGFFMAYGFLLIYLRDFAPGKVEWANSYSIG
                     KHFEARLAHVHGNLFAFLNILIGYLLLRFSDKLKNVKAISWLALTGLFMPIGNLSEVY
                     LGLPPVLVLVGAITMTLSVVWLGFAFLTMKQLTQ"
     gene            24864..25955
                     /locus_tag="PLO70_04380"
     CDS             24864..25955
                     /locus_tag="PLO70_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HQZ73723.1"
                     /translation="MPIFMDLHIVPGVNAKDVAEAHSRDVYLEKDHNCKCLTYWIDET
                     RGHVFCLIDAPNKETVYELHNRSHGLLPHKIIEVQNDFVESFLGRITDPESGQYTNTG
                     LLMLDDTSYRIIMSVNTTDSILLHHQMGLEGAVKKVEKQNTIIRDAIRKHGGKEALHE
                     GRAIIGSFIAAENAVNCGLAIQEQMNGLPDDSFSVQIHAGEPVAQTDELFGDTLQLLQ
                     WLGMLKRKEAIIITDGIKELIAKDMQDKRTVQLLALTLPDEQFLISLFDVLNKNYADN
                     EFSAENYAQILTMSKSQMYRRVTALTGYSPNDLLKEYRLEKAKELLRKKKHNVFEVAL
                     ETGFTSASYFTKCFKTKFGLLPLAYQQLV"
     gene            26064..26447
                     /locus_tag="PLO70_04385"
     CDS             26064..26447
                     /locus_tag="PLO70_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HQZ73724.1"
                     /translation="MKQIFLTSLLLLALTIFSMNNASAQNSNSNTEKIQPALKTYLIE
                     RDIPGAGQLTATDLKGISQKSCTVLKEMGSGISWVQSFVTGNKIFCVYRAENETLLRE
                     HAKKGGFPITSITEINNTISPKTAE"
     gene            26570..27616
                     /locus_tag="PLO70_04390"
     CDS             26570..27616
                     /locus_tag="PLO70_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73725.1"
                     /translation="MKKAILFLVSGFLFACTASSQDAIRKSVPGAKIYFSDKPFTTSN
                     TGSKSSFNSSDFIYGRIELDNKTLQSAFGLPKDGESQMYNKSDCYLRYQVTVFKDGEQ
                     NGQPNFWDFLYVWGNEKNNTAFNFDILPEPAKAKSMLCGTENFSSVIASGPLYHIINQ
                     ERFPDNGTYTIRVRLFQQSADAWGNMEAEEKWPVVEEEFSFNFDTKDIKQLKTNGDAA
                     GELVMENAYRLDKMPEWFSKAGKQNDPKVTNAAIAAILKRDLPTKSIIKFVISEFSGP
                     TWQVEKDEYGVIQQRTFMPTINIAYKRDGKCYIGTVRLWETYEGYGKYGTLIVGSESS
                     SRRSDYWLDCTLIK"
     gene            27777..30986
                     /locus_tag="PLO70_04395"
     CDS             27777..30986
                     /locus_tag="PLO70_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ73726.1"
                     /translation="MKKNYTTVFIAFIMAFNSHAQFFSLAKDINTSALTESSAPNDMI
                     EIGGTVFFSAYTFNRGRELWKSDGTPGGTVVVKDFNAGSATSNPQFLTNVNGVLYFVA
                     RDLVHGEELWKSDGTTAGTVLVKDSYAGDATSNTRNLTNVNGVLYFVAADGTNGFELW
                     KSDGTEAGTIVVKDINAGGFSSNPQFLTNVNGVLYFTATDGTNGVELWKSDGTEAGTV
                     MVKDIYAGTSNSAPGALTNKNGVLYFSATDGTNGQELWKSNGTMAGTQLVKDIYAGPN
                     HSLLFNMVNVNDVLYFSATNAANGSELWKSDGTAAGTVLVKDINPGSNTSALQNLTSV
                     NAVLYFTANDGTNGIELWKSDGTAAGTVMLKDIYAGSTTSSPQLLTNVNGVLYFSATD
                     PDNGTELWKSDGTATGTLLVKDIYATVGNASPQALTNGNSVLYFSATDAAHGAELWKS
                     DGTAAGTQLIKDIWGGTYYSSPRNLTDVNGTLYFSAFEPANGQELWKSDGTQAGTMLL
                     KDLNPGSSSSTPQWLTALNGNLFFTATDGSNGLVLWKSDGTTAGTELLREVAANLPIR
                     SPRYLTVVNNVFYFSAIDYAYGQELWKSDGTTAGTRIVKDINMTGNADPENLTNVNGT
                     MYFTAVSSSFGRELWKSDGTWAGTVLVKDIYPGPIGGTKRYFTNVNGTLYFSADDGVN
                     GEELWKSDGTSAGTIMVKDILPGEIGSSPQNLTNVNGVLYFSATDGINGQELWKSDGT
                     EAGTVLVKDINPGGIGSAPNTLTNLNGVLHFAAADAANGWELWKSDGTTLGTVLVKDI
                     FPGINSSNPVSLTVVNNTLFFTANNGTTGQEVWKTDGTEAGTVIVQDITVQGASNPNS
                     YTLVGSKLFAAITDDVHGEELWVAQTVTVLPLTLLEFNGRLVNNDGLLNWKTTDEQST
                     VSFDIERSTDGRSYISVGNVLAANVTGIHHYHYKDDNVTALGVSKVYYRLKQKDIDER
                     YSYSGIVVLSIDNSRSNVLLYPNPVSSKANVTITLTKPEKIQARIIDNTGRIVKLQQF
                     NLSAGSNSIAIDLGQISKGMYYLELKGETINERKPFVKQ"
     gene            complement(31259..31618)
                     /locus_tag="PLO70_04400"
     CDS             complement(31259..31618)
                     /locus_tag="PLO70_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ73727.1"
                     /translation="MLIEKIRKVIVDYVYNSDEKSTIKFSVLLSKKLNHSYTYLANFF
                     SEVEATTIEQSVIALKIERIKELIIFGEDNFSEIAHKLHYSSAAHLSTQFKKVTGLTP
                     SHFKALKEKRRLTIQNV"
     gene            complement(31608..31817)
                     /locus_tag="PLO70_04405"
     CDS             complement(31608..31817)
                     /locus_tag="PLO70_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQZ73728.1"
                     /translation="MKIYIKNMFCESCKVVVKEALEELDIPSVKVELGEIETRNDVSD
                     EEKMKLNSKIKKAGLELLKKKRYVN"
     gene            complement(32131..32736)
                     /locus_tag="PLO70_04410"
     CDS             complement(32131..32736)
                     /locus_tag="PLO70_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73729.1"
                     /translation="MNIENTWQQSAGSDDALNKMLQTNEFSNLHSKLPLAKLKKNLLI
                     GLILAILITAIYVVLIFFISIWQVHLAIVIAILFNIWVGLDTWKLYTNINANISATNS
                     LKNELQKNYSGFQKWWRIQERLGLFVYPIAATGGFILGGVEGSGKPVEVFLYNPKMLL
                     VLGVTLLILVPLCYYGARWMFNYAYGKHLKKLKQLMDELSN"
     gene            complement(32737..33210)
                     /locus_tag="PLO70_04415"
     CDS             complement(32737..33210)
                     /locus_tag="PLO70_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ73730.1"
                     /translation="MSEKEFLSQLKEHQNIIYKLVHLYANTEEDKKDLYQEILLQAWK
                     TYPAFRGEAKFSTWLYRLCLNTIFTIQRKTNRVEYTDTAQFEEVYSTTPAIDEAERLY
                     RAIRTLPETERAIISLHLDGYDNKEVGEWMGITANLVGVKLHRIKQQLATLLKNI"
     gene            complement(33207..34097)
                     /locus_tag="PLO70_04420"
     CDS             complement(33207..34097)
                     /locus_tag="PLO70_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQZ73731.1"
                     /translation="MKRNFLQAALCLLWPAVIIAQQSKTENALLWKVTGNGLKKPSYL
                     FGTYHFLSSGFVDTLETVKTAYKSADAVVGELVIDTSLQRPMMEAAVLKGTTLQQVLP
                     DTLYAKASDWFKQEAGLDIGQLNQLNPVTVLTAAMAITKGKYFPNQPGEVQLDTYFQE
                     EAKKEGKKVLGLETIQMQIKAMFEQLTLQRQVELLDETFKDKDALRRLIGIMNNAYIS
                     QNLNQLQELMYGSSYRPEEMKILLDDRNNYWMQQLPGLMKDQSLFVAVGALHLVGNSG
                     LVNQLKEQGYTVTPVNLKNK"
     gene            complement(34127..34390)
                     /locus_tag="PLO70_04425"
     CDS             complement(34127..34390)
                     /locus_tag="PLO70_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73732.1"
                     /translation="MKLKSSSETAPKFTPFNRYAYVVYMVLVIYLLIKGDYEWAFSNM
                     GIALLFDPFNPSVKWQQRPIYQKVWLLVHLALTFSGLFYIIFL"
     gene            complement(34788..35438)
                     /locus_tag="PLO70_04430"
     CDS             complement(34788..35438)
                     /locus_tag="PLO70_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73733.1"
                     /translation="MDRYKETFETWNKLASLYQDKFMDLDLYNETYDFVCNSITRVNA
                     KVLEIGCGPGNITKYLLSQRPDFDIFGIDISPNMIELAEKNNPTAKFAVMDSRQINSL
                     EKKYDGIIGGFCLPYLSKTESKELISHSYELLNDNGLIYLSFVEGDPAKSDFKVSGAG
                     RVFFHFHNLGDIKEQLVGTNFDEIETFKVKHKVSETEFDIHTIVTAKKRPVTAVYR"
BASE COUNT        10409 a         7214 c         7525 g        10212 t
ORIGIN      
        1 aaaaattatt aggcctcatc accaaatact atccctcctg atgaattcat tattgaaaat
       61 acatcctgag gtgcaggagg cattgacaaa caacaaaccg gttgttgcat tagaatccac
      121 cataatttca catggaatgc cttacccaaa gaatgtggaa acggcgttgg cagtggaaga
      181 ggtagtaaga gccaatggtg ctgtgcctgc aacgattgcc atctttaacg gcaaatgtca
      241 tgtaggactg accaacgaac aactggaata ttttggtaaa gcaaaagatg tatggaaagt
      301 gagtttacgg gatatgccct atgtgatcag ccagaactta tacggggcaa ccacggtggc
      361 ggccacgatg cggattgcag cgatggccgg gataaaaata tttgtaaccg ggggcattgg
      421 gggagtacac cggggtgcag aaacgagtat ggatatttct gcagacctga cggagatggc
      481 gcaaacatca gtagcggtgg tttccgccgg tgtaaaatct attttagata ttgggttgac
      541 attagaatat ttggaaacaa aaggaatacc tgttattacg tacgggcagg atgaatttcc
      601 cagtttctat tcccgcaaaa gcggatttac ttctccttta cgattggata ccactgcgga
      661 aattgccttg ttgctggaca ccaaatggaa attaggattg accggttctg tattaattgc
      721 taatcccgtt cctgctgaac aggaaatggc aacggatgaa atggaattgc atattcaaaa
      781 ggcattaaat gcggcaaaag aaaaaaacat cagcggtaaa aatgtaactc cctttatcct
      841 gcaatacatt gctgaacaca caaaaggcga aagcctggag acgaatattg cgttgataaa
      901 gcataatgcg aaggtgggtg ccgagattgc ggtggagcat gcgggataaa acaaaaaacc
      961 ccaacatttc tgtttgggtt tctgtggtgt ggggcgggat cgaaccgccg acacaaggat
     1021 tttcagtcct ttgctctacc gactgagcta ccgcaccatt cctgaaacac atcaactttg
     1081 aaaagtggaa gccttacatc gtttttgtgc ttcggggctg caaatataga gggaattcac
     1141 aaaaaatcca cattaaatgg catttaagtt tgttcctaca tcccatattc actcagaaac
     1201 ttgatccgca tgattttcaa ctgttcccag ttgaaattat actcggccaa ttcttcctgg
     1261 gccacctgta aagaggaggt ctcacagctt ttaaaatact caatgatctc atcctggtcg
     1321 tcttcatcca gcatttcatc aatagcatag tccaggttga gcttggtgcc gctggcagca
     1381 atggtttcca tttcctccag catctcatcc atccgccagc ccttattttt tgctattgtt
     1441 tcgagagaaa ctttcttatc tgtattttga atgatataaa ccttgctgcc gcttttgttc
     1501 accacactct tcattacaaa atcatccggc cgttcaatat cgttctcttc cacataccgg
     1561 gcaatcagtt ccacaaaagg ttttccatat tttatggcct ttcccttact taccccctgg
     1621 catttttcaa ggccttccat tgtaatagga tagaaggtcg ccatatcctg cagcgatgat
     1681 tcaaggaaaa taacaaatgg cggcaggttt ttcttcttcg cttctttttg ccgaagttcc
     1741 aggagcattg ccatcagttt ttcatccatg gccgctccgc ctgtagcttc tgttccttct
     1801 tcatcatccg cattagcatc atcatacaat ttattgagca caatctggaa tgatttcggc
     1861 ttcttcaaaa aggcttctcc ttttttggtg aacttaagca ccccatattc ttcaatatct
     1921 tttgtcagca acccttctaa tatcaactgc cggatcaatg agttccagaa atggctgtcc
     1981 ttatcattac cgctggcaaa tacctctaat ccatcgtgac ggaacatttg gttttgtggc
     2041 gtcattctgc caataatgat ctgtaccaca taatccgtag caaatctttc atccagcgct
     2101 ttaatcgttt ttaaagcaat ggctgcctct tcttttgctt ccactctttc tttcggatgc
     2161 ttgcagttat cacactggcc gcaattttct ttttcatact cttctccgaa gtagctcatg
     2221 agtatcttac ggcggcatac acccgtctcg gcaaaagcta ccgtttcatt gatcaattgt
     2281 gctcccactt ctctttcgct gagtggtttg tcccgcatca ggtgttccag tttgcttaca
     2341 tctttgtgtg aatagtggag aatacattta ccctccagtc catcacgacc tgcccggccc
     2401 gtttcctggt aatagttttc tattgatttg ggaatattat aatgaatgac aaagcggata
     2461 tccggcttgt caatacccat accaaacgca atggtggcgc agatcacctg cacttcttct
     2521 cccaaaaaca aatcctgtct ttcggctctt agtttactat ccaggccggc atggtaggca
     2581 acggctttga ttccatttgc atttaatata tcggccagct cttcagtcgt ttttctgtta
     2641 agggtataaa tgatcccgct tttattcttc atccctttaa tgaagcggac aatattttca
     2701 tctgtctgtg cctttttgat ctttggcaat acttcgtaat agagattggc ccggttaaaa
     2761 gaggatataa aaatattagg cttccgcagg tcgaggtttt ttacaatatc gctctgcact
     2821 ttcggggtgg ccgtagccgt tagtgcaatt accggtgcat caggattaat ctgtatcatc
     2881 atttcccgca accttctgta ttcaggccgg aaatcatggc cccactcaga tatacaatgt
     2941 gcttcgtcta cggcgaaaaa agaaattttc agatcagaaa aaaactccag gttttcctgt
     3001 ttggtcagag tttcgggtgc tacatagagc attttcgttt tgccggacag caaatcatca
     3061 tgcacttctt ttatttcctt tttgttgagg gtagagttga gaaagtgggc cacttcatca
     3121 ttgctgctgt agccacggac caggtcgacc tggtttttca ttaaggcgat cagcggtgaa
     3181 acgatcaggg caacaccttc actgaccatg gcaggtagct ggtagcacaa acttttacct
     3241 ccgcctgttg gcatgatcac aaacgtatca tgacctgcca gtaaagattc aatggctttt
     3301 tcctgtgtcc ctttgaactt atcgaaccca aaatattcct gcaaggcttt atgcagatca
     3361 aatcttttct ttgaaggggc cgcttcctta accttcgccg tctttttaac tgtggccttc
     3421 tttgctgttg ttcccatttc cttaattttt cttcatagga gtccatccgg actatttgca
     3481 tttctcttta aatgcagaat tatttaagat actcaatttt gcccgaacgg taataccgct
     3541 caaacagttt tttttcacaa ggtgttccaa tcgttaccag gtataaggta tttgtttctt
     3601 ggtcagtctt aatggccgga gtgtcagcgg gtagtgttaa actaaaccgg gccccgcaga
     3661 agcaaggcag ggtaacacag cgtcatcccc cgtcaattga ggacggcgaa tttacgaatg
     3721 tttacagttt ttagacatgg cgaagcgtta aaattagagt gtatcgattg cataaagggg
     3781 acttcctcgt aaaacctcta attttggcgc tttaattatg gcggtatcaa taaaacaggt
     3841 ggctttaaaa accattgcat tggaggcaaa ctcggtttca ggccttgctt cctatataaa
     3901 tgacgatttt gaaaaggctg tgacagcaat tgcatctggt aaaggccggg tagtggttag
     3961 tggcattggt aaaagtgctg tcatcgcaca aaagattgtt gccactttta actctaccgg
     4021 cactccatca atttttatgc atgcggcaga tgccattcat ggcgacctgg gcatggtgca
     4081 acaaaatgat gtggtagtta taatcagcaa aagtggcgaa agccctgaga taaaagtgct
     4141 ggtgccattg ataaaaaact tcgggaatgt tttaattgcg atggtcggta atattgaatc
     4201 ctaccttgcc cgcaacgcct ctattatttt aaatactacg gttacacaag aggcttgtcc
     4261 caataacctg gcacccacct ccagtacaac ggcacaactg gtcatgggag atgcactggc
     4321 gatctgcctg atggaattga acggtttcca atcggaagat tttgccaagt ttcatcccgg
     4381 gggttcattg ggaaaaaaat tatacctgcg ggtggttgat ttatatgccg acaatgaaaa
     4441 accaaaagtg ctggcagaac aatccctgaa agaagtgatc gtggaaatga cggcaaagag
     4501 attgggtatt acagctgtgg ttgatacttc gaataatcta ttaggcatta ttactgatgg
     4561 cgacctgcgc cggatgctgg aaaaaagtat cgctattgat acaatcaaag ctggtgatat
     4621 tatgacagtt aatccaaaaa caatcgggcc ggatgagctg gcggtggccg ccctggatgt
     4681 gctacgtaaa aatgaaatca cacagctggc agttacagat gcaggaaagt accttggtat
     4741 aatacatttg catgacctgg taaaagaagg attgatttaa ccggtgagcc gtgtcagcat
     4801 tcctgttcac gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcaaacc
     4921 cacctatcca aatacagggt atggttatat acagcatgat accgccgaag cagctccaga
     4981 tgtatacaaa gtaaaaacct ttacagaaaa acccaatgtg gagcttgcaa aagcatttat
     5041 ttccagtggt gattttttat ggaatgccgg cattttcacc tggaaagtaa agaatgtttt
     5101 aaccgctttt gaaaaatacc tgcccgaagt atatgaggtt tttgcagcgg aaaaagacaa
     5161 gtttaataca gacaaagaaa aagaggccat tgaaagcatt tatccgcaat gcaccaacat
     5221 ttccattgac tttggtgtaa tggaaaaagc ggaaaatgta tatgttattc cggccacctt
     5281 ttcatggagt gacctcggca cctggaacag cgcctgggaa aacaaagaga aagactattt
     5341 cggaaatgcc gtggtgggta aaaaagtaat ggcggtggat gcccgcaatt gcatggtgca
     5401 tgttccggat aacaaattgg ttgttctcca gggtcttgat gaatatatca ttgtagacac
     5461 caaggatgtc ctgctgatct gtaaaaaaga taaagaacag gagatcaaag aatatgtggc
     5521 ggaggtaaaa cgaaatatgg gagataaata tttgtagtaa tcatcacaaa ataattacct
     5581 gaaagtagta gccccggttt gtccggggtt ttttactttt aataagaaat aaactgaagc
     5641 ctatgtatcc cgttgcctct attctcttcc tggatattga aactgtaccc cagtattcgt
     5701 cttacaacga gcagcctgaa gagtggaaaa ctctctgggg gctgaaagca caatatctaa
     5761 tccgcaacaa agaagaagaa acggttgaaa ccatttatcc ccgggccggg atctatgcag
     5821 agtttggtaa aattgtttgt atcagttgtg gttttataca aggtgcgggt actgataaaa
     5881 aaatcatcct gaagtctttt gccggggacg aggaaaagaa attgctgtat gaattttcag
     5941 aaatgctgaa gaaatggtcg gccgacggaa gtaaatttct gtgtgcccac aatggcaaag
     6001 agtttgattt cccttatctc tgccggagaa tggttataaa ccaaattgcc attccctcca
     6061 ttctgaacat gtcaggaaaa aaaccctggg aggtaaacca cctggatact atggacctct
     6121 ggaagtttgg tgactttaaa aactatacct ctctaaacct gctggcccat tcgctgggca
     6181 tacaaacacc caaagatgat attgatggaa gtatggtgtg ggaagtgtac tggaaggaaa
     6241 agaacctgcc ccgcatcgta acctactgcc agaaagatgt ggtgacagtg gcacagatct
     6301 ttttaagaat gaacggggaa cctttgatta gggaagaaaa cattgaaata aaaccttaat
     6361 gaaaagaata aactattcct ccggtgccaa atgggaagat atcgttggtt acagccgggc
     6421 agtaaaagtt ggaaacatca ttgaagtaac cggtacggta gcagtggatg aaaacagctc
     6481 attggttgga aaggatgatg cgtatgccca aactaaattc atccttgaaa agatagaaag
     6541 tgttttagaa aaagcaggcg cctccttaaa agatgtggtg agaacgagga tgttcgtcac
     6601 cgatatatcc cgttgggagg aatatggcaa agcacatggt gagttttttg caaccatcaa
     6661 accctgcact tctatgatcg aagtaaaagg gttgatcgcc ccggaatacc tgattgaaat
     6721 tgaggcaacc gctatcatat cctgatatga atattgaaac actaagagaa tattgtttgt
     6781 caaaaccagg agcagaagaa acgctgccgt tcggtcccga taccctggtg tataaagttg
     6841 gtggaaagat gttcttatta acaggacttg atagcgaaga ccttcgtttc aacgtaaagt
     6901 gtgacccgga taaagccatt gaatggagag aagagtttac ctgcgtgcaa ccgggttatc
     6961 atatgaataa aaaacactgg aataccattg tagcagatgg ctccgttccc gataaacaat
     7021 taaaagaatg gatcgatcat tcgtatgagc tggtggcaaa aaagaaaaaa taactcaaac
     7081 gaatatttct ttaatctttc ccgccacttc cagtccggca tttttacccg tatcgttaag
     7141 caggatttct tttgttttgt tatcggtcaa caatacctct attgccgatg tcatactttc
     7201 ttctatcctt ccttccataa atccacggat aggtgacgcc agctccgtgg ctgtatcccg
     7261 gtgagctaaa atttcaagcc ggtactgttt attctccagc acaattttta cctttttctc
     7321 atcagcaaaa gaggtaagca gttttgattg attgtaagtg gtaaacttta tcagcctgtt
     7381 gtgtaaccag ataccagcaa taaaaccgac aaaggaactg cggacccagg gaatttttgc
     7441 aactgaacat tttaaggaaa tgcccggctg actgaaatgg tttgattgca tccagaaata
     7501 tgcagaggga aaggatcttc cccaatcttt ttctatataa ccgcggccat tggtaaaatc
     7561 aatggtttcg ccgttgacaa tgatcatccc gtccacccgg tgatccatgc ttacaattcc
     7621 atgataacat tccataaaag gaacaaatgc atacggcccc ataattcccg gcgaatacca
     7681 tggcttaggc cagggaatat tcccggtaaa ttgaagttcc ccggttaatc ccggtaaact
     7741 gagctgaata cccgatccgg agaaagagtt attttgaatg ctcaccctaa agtttttagg
     7801 gagggagtaa aaatcatccg cagtaaaccg gtgatagccg gcagtttgtt ttttcccatc
     7861 cagcacctgg atgaaagaat gtttattccc ggcagcatcc attgcaatac caggtataaa
     7921 agcaaatgct tttgttgcat cggcattcac tactttaaaa taccatcctt caaaatagtt
     7981 ttttgttttc ccccatccct ggaactgttc gggattgaaa aaggcatgaa ccttattgaa
     8041 tagcattgtt caatatacaa aacttacagg gctgcgattt cctaaatggg gttaatttta
     8101 tgctgttcgc atttcaataa ttacaattat tcacattgag tataccgcat atacattcca
     8161 agctgcccaa tgttggcact actattttta ctgtgatgag tggattggcc acacatcaca
     8221 atgcagtaaa cctgggccag ggctttcctg atttcccgat gaacgaagaa ctcacggagc
     8281 tggttaactg ggccatgaaa aataatttta accagtattc gccaatgccc ggctggttcc
     8341 cgctgcggga gacgatagcg gagaaagttg agtttttgta ccacacaaga ataaatccgg
     8401 atactgagat caccatcact cccggcggta cctatgccat ctattctgca ttcaccgcaa
     8461 ttcttaaccc cggagatgaa gtaattgtgt ttgagccggc ttatgacagt tatattccga
     8521 atattgaaat caatggggca aaagccatta ctatcaacct ggtttacccg gattaccata
     8581 ttgattgggc cgctgtcaaa aaagcgatca catcgaagac aaaagctatt atcatcaact
     8641 cgccgcacaa tccaaccggg agtgtgatta cagaaaatga tatacaggag ttgaggaaaa
     8701 tagtggaggg aaccaatatt tttatcatca gcgatgaagt gtatgaacac ctgatctttg
     8761 atactattcc acaccagagt atacttcgct atcctgattt gttacaaagg agttttgtat
     8821 gcttctcatt tggtaaagta tacaactgca ccggctggaa gctgggctat tgcatcgccc
     8881 cgcccgagct gactacggaa ttccggaagg tacaccagtt caattgtttt agctgccata
     8941 ccccttcgca ggtagcgctg gcacagtttt tacaaaataa agaggcctat ttatccttat
     9001 cctcatttat gcaggagaaa agagattact tcagtaagct gatgaggggt acaaaatttg
     9061 aaatgctgga tagccatggc agttatttta tctgtgccaa atatgatcgc atcagtgatg
     9121 agcctgataa agagtttgcc atacgcttaa cgaaagaagc cggggtggcc actattcctg
     9181 tttctgcttt ttatcaaaac ggaaaagacg ataaagtctt acgattttgt ttctccaaga
     9241 aaaatgaaac gctggaaaaa gctgttgaaa aactcnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnntacac attgctttat tgtattctgc atattccgct gggtacggcc
     9421 atgacgtata atcttacttc cgccttattc attgccgtat tttcttttct tcttttccgg
     9481 gaataccatg gcagggtggt acttttggct gtattgcttg gttttctggg tatgttgctg
     9541 atctataaac cagccatgca cttcccctgg tattatcatg cagcagggct tatttcgggt
     9601 gttgcttccg cccttgcata tattactgta aaccggttgg ccggttatta tgactcgagg
     9661 atcatcgtgc tggcttttat tggtacagga gttcttgtgc cccttgtctt tatgataata
     9721 agatatagtg ccgatatacc gccagatgat gtgttcttta tttcatggaa atggccttcg
     9781 ggtttcgaat ggttttatgt gctgtggctc ggtttagcag ccctgtttgg tcaatatttt
     9841 gtcaccaaag catatggagc cgataaggca ggaattgttt catctatcgg gtatgccaat
     9901 atcatctttt ctgttttcat cggtatggcg ctgggtgatg ccttccccga ctggatgtca
     9961 agccttggca ttctgtgtat tatcagcagc ggggtgctca tttctgtctt caaaacaaaa
    10021 aataaagttt cttaagaaat ttttttcaaa aattatttgt gcgtttagtc acactcatcg
    10081 tatatttgcg atataagata ataaaatggc ttttcataaa aaagaagaat ttacccaaaa
    10141 agaacaggac ctggccaatt ttgccaaggc catgagccac ccggcaagga tcgccattct
    10201 gaaggtgctg gcacaacgaa acgaatgtat ctgcggtgaa ctggttgata tgttgccgct
    10261 ggcacaaagc accgtatcac agcacctgaa agaactaaaa aatgccggcc tgatcaatgg
    10321 tgaaacagac ggccccagga gctgctattg tatcaactgg aaagcttttg aaaaattcaa
    10381 taacgaattc aatcttcttt tcagcaacct gaagatcaaa aatgaaaaag cctgttgctg
    10441 aactgattta ccacttaaaa atagtaacca tgaacacgga tgcacaacta aaagaaatag
    10501 taagacagaa atacagcgaa atagcgctgc aggataaaga aactaaccag tcctcctgct
    10561 gtggtgccgg tggctgttcc actgaagtgt ataatatcat gagtgatgat tatactacac
    10621 ttgaaggata taatcccgat gcagaccttg gtcttggctg tggcttgcct acccagtttg
    10681 ccaacatcaa aaaaggtgat acagtgatcg acctcggtag tggcgccggt aatgattgtt
    10741 ttgttgccag ggccgaaact ggtgagacag gaaaggtgat cggtattgat tttaccccgg
    10801 ctatgattga caaagcaagg gcgaatgccg agaaaagagg attcaataat gttgaattcc
    10861 gccagggaga tattgaaaaa atgcccgtta cctccaatgt ggcagacgtg attgtcagca
    10921 actgtgtact caatcttgta cccaataaag acggcgtgtt caaagaaata ttccgggtat
    10981 taaaacctgg tggtcatttc agtatctccg atgtggtatt ggttggcaat ttaccggaag
    11041 cgctgcgcaa agatgcagag atgtatgccg gttgtgtgtc gggagctata caaaaacaag
    11101 tgtacctcga attgatccgg cacaatggat ttcagaatat tacggtacaa aaagaaaagc
    11161 cgatcatcat ccctgatgat attctgaaga attacctgga cgacgaagag ctgaataaat
    11221 tcaaaaacgg aacaacaggg attttctctg taactgtttt tgcacaaaaa ccggcctgta
    11281 cgccgggcgg tggctgttgc taaatcgtct cttatttttt caccataaat agtatagcca
    11341 tgaacacaaa cattcaatta cacaacgacc ccaataatgg cggtggttgt tgcggcggca
    11401 caggagactg gggctgttgc taagatttca ggaggagggt gctaccctcc tctcttttaa
    11461 accaactaac ttatgcccaa aaaactttct tttctggacc gctacctcac cctgtggata
    11521 tttcttgcga tgggtattgg cgtggcaatt ggctattttt ttccagcggc tgaatcattt
    11581 atcaattcgt ttcaatccgg cagcaccaat attcccatag caattggttt aatactgatg
    11641 atgtatcctc cgctggccaa agtgaagtat gaagagctgc ccaaagtatt tgcgaataaa
    11701 aaaatattac tgctctcttt agtacaaaac tggatagtgg gtccggtcct gatgtttgcc
    11761 ctggcggtta tttttctccg ggatgaaccc ggttatatga ccgggctgat cctgataggt
    11821 attgcccggt gtattgcaat ggtgattgtc tggaatgacc tggccggtgg tgaccggctt
    11881 tatgcggcag gtttggttgc gttcaacagt atcttccagg ttgtattcta cagtttttat
    11941 gcctggatat ttattaccaa acttcctcct ttgctcggat taaaaggata tgaagtcaat
    12001 atcacgatcg gtgaagtagc acaaagcgta tttatttacc tcggaattcc cttcctggcc
    12061 ggaatgatct caagatatgt gctgatcaaa tggaaagggg aagattggtt taccaaaaaa
    12121 tacctgcctg ctataagccc cattacattg attgccctgt tgtttaccat tgttgttatg
    12181 ttcagcctga aaggaaacat gatcgtagaa attcccggtg atgtgctgct gattgcaata
    12241 ccactaacca tttatttcgc tattatgttt ttcacagcgt tctacttatc aaaaaaagca
    12301 ggtgcagatt acgaaaagtc tacttccctg gcttttacag cagccggaaa caactttgaa
    12361 ttgggcattg cagtcgctgt atcggtgttc accattcact cacctgttgc attcgctgct
    12421 gtgattggac cacttattga agttccggta ttgttattat tggtaaggtt tgccctgtca
    12481 caacaaaaaa aatttaaacc agcaaaacaa tgaagaagaa actccttttt gtttgtatag
    12541 agaacagtaa ccgctcccag atgagccagg cttttgccaa aattctcggc ggtgcaaatg
    12601 tggaagccta tagtgccggc tccaaacctt ccggtattgt aaatccaaaa gctattgcag
    12661 cgatgaaaga gctgggttat gatctcagcg cccacgacag taaatcactg aaagaagtag
    12721 agcaatatgc tccctttgac gctgtgataa cgatgggctg cggtgatgct tgtccctgga
    12781 tgcccgctaa aaaatttatt gactggcaaa ttcccgatcc caaacacatg gaacccggag
    12841 aattcaacaa agtaagagac ttaataaaag aaaaagtagc cggcttactt gaagagttag
    12901 aaaaaattta gtcgatatac ttttcaggaa taagattatc cggtgtttca gcctgccaga
    12961 aaacagtaag gatccaccat cgcttaccat cattccacag ctggatgctg ttaatgcccc
    13021 gcatgaatgg tttttcatca ttcagctttc ttttggaatc gtaggtacta aaaacatgaa
    13081 ccacgctgcc aaactgttct gttcttcttc ctatctccct ttcaaaaaaa ccttcttttt
    13141 ccaggaatgg gcctgatgtg gcaatataat cctccaccgt cattattctt tttcccatag
    13201 taccgtccga ttttttaccg gtcgccatca atcttccttc cggcaaaaac aatgtcctca
    13261 tccggtccca gtttctcttt tcgccagctg gtccggagat aacgttatat aaagcggcaa
    13321 ccagtgcatc tggcgattcc acatctttag gattagccgg aatactatcc ggccggttat
    13381 tggcaaacag ggaaatggtg caaaagaaac tggtaatgat gattaaataa gagacagtct
    13441 tcattgctgg tattttggat caagttacag ctattcagtc ctggaaaaat tccattcatc
    13501 aaactcctgc ttctttactt actttgccaa aacgtttacc ttttgaataa cgctttattt
    13561 tcccgcaacc tctatatttt tctcttctcc ctgttaggca ttgtatatat caccggcttg
    13621 tttgttccgt tgatggataa tgattcggcc caccatgcaa atattgcctt gcgtatgtac
    13681 ctcaccggcg attatgtgaa cctggtcgac tatggaaaag attatttaga taagccgcac
    13741 ctgcacttct ggctggctgc tttcagttat aaattatttg gtgtaaatac atttgcctac
    13801 aaactcccct cccttttatt taccatcata ggcacatatt ctacgtatcg ccttggaaaa
    13861 gctttatata cccatgagac aggaaaactg gctgccttat taacagcgtc tgcttttgct
    13921 tatattcttg ccaataatga tgtgcggatg gatgctatcc ttactgcctc catcgctatt
    13981 gccacctggc agctggtaga atttattcag cataaaaaaa taatgaacat tgcgggagca
    14041 gcgctgggcc ttgctcttgg attctgcacc aaaggacata ttgccgtatt cgttcctgca
    14101 gtggctgcct tcttttatat actatacctg aaagactgga aactgttgct aaattggaaa
    14161 tggcttttat taatagcttt attcgggttg ttcatatcac cggtggtgta ttgttattat
    14221 ttgcaataca accttcaccc cgaaaaaata gtacggggta aggatcaaat caatggtgta
    14281 aaatttattt tatttaacca gagtgtggag cggttcagcg gtgaaatggg aagcgatgcc
    14341 aaaaatgatt atttcttttt cctgcattcc tttttatggg cttttgcacc ctggagtatc
    14401 ctcacctata ttgcgatttt cggaaggcta aaaaattttt tacaccgaaa agaagaatgg
    14461 cttacaacag gtgtgttcct ggtaatgctg gtggttgttt cattgtctgg ctttaagctg
    14521 ccgcactacc tcaatattgt ttttccaacc tgtgcagtga tggcggcttc attgctcctg
    14581 caaaaaagag aagagccgat gtgggtaaaa aatattttcc ggctgcagct gttcatctct
    14641 tcactactgt tggtgctggc agctgttatt aatttctggg ccttcccggt ggaaaatcac
    14701 cgggtaatga tcctgctggt actcttgctt actgtattct tttatttttt taaaacaaca
    14761 acgctgcggc gtattcaaaa aacagtagct gtttctgtta cggcaatggt gctgtgtttt
    14821 tttctgctaa acgctaattt ttatccgcaa ctattacgct accagggcgg gaatgagctg
    14881 gccgctgcca ctaaagaaac ggtaaatccc gatgacgtat acttctggaa agagaattac
    14941 agttcttcct ataatttttc aacgtccaca ctgcgtaaac cctttactga ttcaatatgt
    15001 caggagggta aaaaaatatg gctcttatat gatatccgta acgaagaaga aataaagcag
    15061 gccgggtaca aaataggcga aaagtattca acccgggact atgaaataac caagctggat
    15121 ttaaaatttg tgaatcccca gaaaagagac agccagtgta caaaaatggt gattgcggaa
    15181 gtgagcagat tatgagaaat accttataat gccagaaaat gaatataaaa aaattatagt
    15241 caaatttatc aacccgttct gtaattacct ttctggttaa atcataaacc tgccctggca
    15301 gaaatctcca gcatccggtc aatcggtttt ttcgctgcta atcgcaattc ttcctccatt
    15361 attatctccg gcaattcata tttaatacag agataaagtt tctccagtgt attcagcttc
    15421 atgtgcgggc aatcattaca agcacaatta ttattaggag gtgccggtat aaatgtttta
    15481 cccggcgagg ctttcatcat ctggtgcagg ataccggtct ctgtggctac gatatattcc
    15541 tgtgatgggt ctgtttgtgt gtattttaat aaacctgttg tggaacctat aaaatctgca
    15601 atacgaagaa ccgggtcttc acactccggg tgtgcaatca gttttgcatt cgggtgcctt
    15661 acttttagtt tggtaatttt ttccagacta aatatctcat gtaccataca ggcaccgttc
    15721 cataacacca tatctcggcc ggttactttg ttgatataag cgccgagatt tttatccggg
    15781 gcaaaaatta ttttctgttc tttttggaaa ctttccacca caatttttgc attactgctc
    15841 gtgcagatca catcactcag tgctttaatg ccggcactgc agttgatata actcaccacc
    15901 acatgatcgg gatgttgttc tttgaatttt ttaaataacg gtggcgggca actatcgctc
    15961 aatgaacacc cggctttcag gtcaggaatc accacttttt tagcggggtt aaggatcttg
    16021 gcggtttccg ccataaaatg taccccggca aaaacgatca tatccgcttt tgtcttttct
    16081 gcctgttgtg ccaggcctaa actgtcaccg atataatccg ccacatcctg gatatccggc
    16141 tcctggtagt aatgcgccag caacagcgca tttttttcct tcttcagttt ttcaatttct
    16201 gcaaacaggt ccagtgtagg atcgagttca atatcgagga aacctctttt ttcaatagct
    16261 gccctggcgg tatccaatgt gtcagtgttc atctttttta ttttcgttcg atgaagcaat
    16321 attccaacaa gtcacacaac ttttcaaaac aaaatttttt aacccgggta ttaatacaat
    16381 aatagtatta atatattttt ataaaaaaga gtatattatt attacagctg tggattcgtg
    16441 gaaaactatt tttagttaac aggcgtaaag ttacactttg tcagatattc acattgtaaa
    16501 caaggaaatc cacaacctgt tttattacct gtactgtatt tcagcctgct tatccacagt
    16561 aatgtgaaat accaatgtgc taaaattttc tataccatct tataaaaaag agtgtgaaca
    16621 gaaatagaat aacttgtcaa atttgtacac tgcgtcttga atcatggtga tgctcacatt
    16681 ttcagttcca aacttactgt ttcatatcca cgttccacat ggggttattc acataaaata
    16741 ttctctgtgc acaggatgat ggggagaaaa aacagggttg cttacccgtt ttaaaattat
    16801 tgaaagggaa aataatttaa tgtgttgacg aaaatcatcc gttttactaa atatataagc
    16861 ggaaaatcct ttattcgcta ataccctaat tttacattca cacaaaaatt ttccatatga
    16921 aatctcttaa acacctgctc gtcgcttttt ttctttttgt ttttactata gccagggctg
    16981 atgaaggaat gtggttaccc ttattcctgc agcagctgaa tgaaaaacaa atgaagggaa
    17041 tgggaatgaa gatcagcgcc aaagacattt ataatattaa cagtggcagt ttaaaagatg
    17101 ctatagtgag ttttggtggt ttctgcaccg gtgaagtcat ttcttcaaag ggactggtat
    17161 taacaaatca tcattgtgga tttgacgcca tacaaaacca ctccacactg gaacgtaatt
    17221 atataagaga tggtttctgg gccaggaacc tgagcgagga aattcctaac cagggtttat
    17281 ttgtcacttt tattgtacgt attgaagacg taaccaaaca ggcattgaat ggtgtaacta
    17341 aaaccatgag tgaaagtgaa agacagtctg ctattgataa gaatatagcc gggctgagaa
    17401 aggataataa gagagaaact taccaggata attttgtccg ccctttcttt gaaggcaatc
    17461 aatacttttt gtttgttaca gaaacatatc gtgatataag attggtgggt gccccgccat
    17521 ctgccattgg taattttgga aaagatacag acaactggat gtggccccgc cataccggcg
    17581 atttctctat gttccgcatc tatgcaggaa aagataataa accagcccct tattctacag
    17641 ataatgtgcc ttatataccg aagcgatcct tatctatctc attggacggt gtaagcgaag
    17701 gggattttac aatggtgttt ggttttcccg gaagaaccac tcaatattta ccctcaccag
    17761 cagtagacca gataatgacg gtgaatgacc cggccaaaat tgcaatcagg gataaagcac
    17821 tggcagtgat cgatgggttt atgaggaagg atgaacaaat taaaatccag tacgcagcca
    17881 aatatgccag catcagcaat gcctggaaaa aatggcaggg cgaagtgctg ggactgacca
    17941 gcacgaatgc tgttggaaaa aagaaagcct atgaagcaga ttttcaaaaa agagttaata
    18001 caaatccgct atggaaaaca gcgtatggca acctgttggg tgatatggaa tcacattacg
    18061 caactttaaa accttacggg tatgcccgtg attattttac agagatcgtt agtaaaatag
    18121 aactctttac tatcgcagcc cagttaaacg cactgataac ggcttatgaa aaagacggag
    18181 agaacggatt taagcaacga ctacctgtca ctaaaaatgc actggaggga ctgtatactg
    18241 agtatagtcc ggttgtagac cagcagttgt ttgaagtgct gatggaaatg tatgtaaaag
    18301 accaggacaa aaaatatgtt tcgcccatgc tgatagaatg gctgaatggg gatatgaacg
    18361 ggtttaaaaa actggccgct aaagtatatg atgctacccc attggatgat agcaaggcaa
    18421 ccatgaaaat gctggaaaaa cccgccgctg aattggtagc cgaactaaag aaccaacagg
    18481 tgataaggtt gtaccaggac cttttaaaaa cataccaggc agaggtacaa tcaaagctgg
    18541 gcgaaataaa cagtaatatc aacaagttgc agcgtacata tatgcaggca cagatggagg
    18601 tgatgaaaga aaagaaattc tacccggatg ccaacagcac attaagggtt acttatggta
    18661 aagtgaaagg gtacaacccc agagacgcgg tgaagtttga tttttataca tacctggatg
    18721 gggtgatgga taaatacaaa cccggcgatt atgagtttga tgtgccggaa aagctaagat
    18781 cattattcaa atccaaagat tatggccagt atggaagcaa aggaaagatg cccgtatgtt
    18841 ttatcgccat gaaccacact accggcggta attcgggcag tccggctctg gacgcctatg
    18901 gcaacctgat cggcctcaat tttgatcggg tttgggaagg aacgatgagt gatatcaact
    18961 atgatccctc tatttgccgg aatatcatgg tcgatatccg gtatgtgctc tttatcgtgg
    19021 ataaatatgc aggcgccggc cacctggtaa aagagatgaa actggttcac cccaagaaaa
    19081 agtaaaatcc ccccgaagca gctataaata ccgattacaa ctcattcaaa atgcctcctc
    19141 cgggggcatt ttttgtggat tttccacagt ttttccccta tttttgccat cctttaaaaa
    19201 agcaggataa tataatatta tgtctattat tcaggatatc cgagataagt atgcaaagct
    19261 tactgtagtg ctgattgcat tggcccttgt gggatttatt ttaaccgatt atttttctgg
    19321 ccaggcaaga gccggcggcg gcggtgcatc cagcactatc ggtatggtga atggccaaaa
    19381 aataaacttc gacgagttta acccgaaggt tactcaaatg gaagaaaata tgaaggccca
    19441 gggttatccg cagaatgcgt cacttaccca gcaagcgatg gagcaggcat gggcacagga
    19501 aataaaccgg ctcctgttaa cggacgagtt taaaaatctc gggattgaag taggtaaaaa
    19561 agaattgggt gatattcttt acggcgctaa cgcaccacag gatatcaaac aacaatttac
    19621 cgatgaaaaa acagggcaat ttaatgcggt gctggccaag cagaatatcg accaggtgtt
    19681 gaaaaaagga acaccggaac aaaaagcaaa catcagcaac tatatcaacc agctggctga
    19741 actaagaatg tctgataaat atttatcttt actttccaac agcacgaact ttccccgctg
    19801 gatgattgaa aaacaaaatg ctgataacag tcagttggca aaaatatcat tggtaagaga
    19861 agtgtacagc agcattgcag acagtacgat caaaattgaa gacagtgaaa ttgcggcgta
    19921 tatcaataaa aataaaagcc agtataagca acaggaaagc cgtagtataa attatgtaac
    19981 cttcagcgct gcaccgtctg ctgcagacag tgcagaagta aagaacaggc tggttacact
    20041 gaaagagaat tttaaaacag cagaaaacct ggaacagtac cttgcaggag aaggcgcaaa
    20101 ttattattcc ggctatatca gcggtaaaac aatccagata gcactgaaag attctatttt
    20161 caaaacgctg gttggtgaag tatacggacc ctatcttgat ggcggcagtt acgcattggc
    20221 cagggtgttg ggggttaggc aaatgcctga tacagctacc gtacgccata tacttatcag
    20281 tacacaatca agagattctg caacaggata taacctggcc gacagtttga aaaatgctat
    20341 tgccgcgggt tccggttttg attcgctggc tgcaaaattt tctgatgatc ccggtagcaa
    20401 ggataagggt ggtttgtatg aaaatgtacc ttccggacag atggtaccga ccttcaacga
    20461 ttttattttt ctgaacccgg taggcgctaa aggaatagtg aaaacggatt tcggttttca
    20521 ttatattgaa atactatcac aaaaaggcaa tggagctgct tataagattg cgtacctgcc
    20581 aaaggaaatt attgccagcc aggaaacaga taatgcagcc ctgaataaag ccaatgagtt
    20641 tgcaggcgac agccgcgatc tgaaggcctt tgatgctaat tttgaaaaaa cactgaaacc
    20701 gttaggaatt cagaaaggca tcgcaacaga tataaccccg tcttcttcag atatccgtgg
    20761 acttggtgtt tccaggtctt ttgttagaag tatctatgaa gctgataaag gggatgtgct
    20821 gaagccggaa cgtattgacg aaaactatgt agtggctgtg gtcactgaag tttttgaaga
    20881 aggaactatg agtcctgcca gggcaagagt gtctgtagaa ccagccttac ggaataaaaa
    20941 gaaagcggaa atattaataa agaagatcgg aaccgtgact acgctggaag ctgctgcaac
    21001 agcattaggc ggtaagacca tagaaacagc agacagtatt cgtatcaact ctgcgttgag
    21061 ctctactctg ggttatgaac caagagtaac cggcgcagca ttcaacccgg ctaataaagg
    21121 gaaagtagtt cctgcagcta ttgaaggcat gaacggagtg tatgtggtga gagtagagaa
    21181 tgttactacc acaccagtta ctgatggtga tgtgaacgga caaagagccg cccgttacca
    21241 gcaggcaaaa caatcgttta gtaaccagta cagccctaat aacccgctga gtattttgcg
    21301 caatgcagca actatcaaag acaagcggtc taacaggtat taagaagagt atagcaacca
    21361 aaaccaactg ccgccccgta tcaggtaaac cgatacgggg ctttctttta cggatgtcgc
    21421 aaaccatagc cgcccgtaaa ttgttatttt tacaaatcat taccgggcag atttcatcga
    21481 actgttttga ttagttcttc gataactaac cggcgatagt aatagcatga gtgaaccctt
    21541 tatcaataaa gtagctgaaa gtggattgat aacactggac ctggagaaat ggtatccccg
    21601 gggtgaagcc gccgtgtttg atatgaagga ttatttgttc atggggatga ttctgaaaga
    21661 aaaagatttt cgtgaagcat taaagcagat ggattggtcc gtctacgaag gcaaaaacgt
    21721 tgccgtcacc tgcagtgcgg atgctgttat tcctgtttgg gcttatatgc ttgttgccgc
    21781 ttacctgcaa ccagtagcaa aggaaatagt aatgggcgat gaaaaggagc tgcacaaagc
    21841 gctgttctta aaaaatttat cctctattaa tttggaggaa tacaccgaca aacgaatcgt
    21901 gataaagggc tgcggcgaaa ctcccattgc cgattatgtg tacatggaga tcactaaact
    21961 actgaggcct gttgtaaaaa gtatcatgta tggagaaccc tgcagcacgg tacccgttta
    22021 caaaaagaaa taatagcaaa agaattctct actgctaacc gttactacac taaccgggat
    22081 cagaaccacc ctcttttctt aaaccatacc agcatggcca gcggaaccag tatcataaaa
    22141 gaaattgcaa aataaaagcc ccattcatga tgggaatagg gaatgacctc gaagttcatc
    22201 ccaaacacgc cgccgataac agtagccggc gccagcaggc aggtcacaat ggtcatcact
    22261 ttcatgactt cattcagccg gaggtttaca ttactgatat agagatcctg cacactcatc
    22321 attacatcac ggtagttttc actcaggtca aacgcctgta tgatatgatc ataaacgtct
    22381 ttaaaatact tggtcgtcct gtcttccagc aattcacttt cactccggat gatacccgcc
    22441 accaggtctc ttaccggcgc aatatttctt ttaagtacga tcagctcttt gcgtaattga
    22501 ttgatgcggg ccagcgatcg cttattactt ttccgcacca cttcttcttc caatgcttct
    22561 atctggtcgc cgagccgctc catcacaatg aaataactgt caacgatcag gtcaatcatt
    22621 gcatacagca ggtaatcggc agtacgttgc cgcaactggc tcttggtcat ctttagccgg
    22681 tgccggatag gatcaaatac atcccgtgcc ggatcctcct gaaaactgat cacataattg
    22741 gtgcccagca ccacacttat ttgttccgtt tctactgttt tcttttcttc attaaagtaa
    22801 agcatgttca gcaggcagta gagcacacct tccacttcat ccattttggg ccgctggtcc
    22861 acactcagga tgtcttcaat aatcaacggg tgaatgacat attttgtaca aagggcctcc
    22921 acatctgctt tgcggatgcc atcaatattg atccaggtga tggaagttga ttctttatag
    22981 tgaaatacag tatctactga ctccgctttt ctttcttcca gctgttcagc agtatagtca
    23041 tagacggtca ctacaaccct gtctgcttct tcacggtgcg gaaggatggt ggggtttaca
    23101 gacagaattt cttttgttcg acgggtgccg aacaggttgg ggggatacag gtatttcagg
    23161 tatttttcgg gatgcagctt tacgttcata ataaggaaag ttaggaaaag aaataaagca
    23221 aaaaacactt gttacggtta tatatactac ctggccgccg gagccaaacc aatgtacatt
    23281 tttccgggca gtttaatttg cttactgctt tcttatctga tgtgtagttg cagtgctgga
    23341 ttttatcatc ctgtaatttt ggattacagc aagggaaacc gccgttactc gccggcgaaa
    23401 atctaattat ttaatgagta ttaagttgct ggtagatatt gcatttatcc aatgcctggt
    23461 attaggttcg atgttcacat aatcacctgt tgtcagcgta agtttaattc cattttcatt
    23521 ctcgaaaagg gcttcacctg ccaggcaaac taataatgcc ggtacttttg taacatgttc
    23581 ctttaattgt tcaccagcca gtatatggat ggaggttact gtaccttctg ctgttttaaa
    23641 taacgaagcc gttgaaactg actttgtatc tgtatgtaaa tcttttatgt tcatggtttc
    23701 ttctttttat ttatggtaaa ataggcaaaa agagtgaagg caactgtcag cgcaattctg
    23761 aaaaccattg caccaaccgt tttggtttcg tgaataccgc ctgattggat ataaaaaaga
    23821 aaaccaataa aggcaatggt taaaataata gcggaaagac ctaatatttt gtaagtccac
    23881 ttttttgagg taataagtcc gtatactgca accaggtaaa gcaaactgct gatgaaattg
    23941 gaccaaacaa caaataaaac atagttacct tcttttgacc tgatgtcaaa caaatcaaaa
    24001 attacggagg cgctgaggaa tagtgtcaac aagccaaagc ctgtcaaaac cgcagctact
    24061 aaataaagta taatcttttt cattattggg ttaactgttt catggtaaga aaagcaaagc
    24121 cgagccaaac aactgatagt gtcattgtaa ttgcaccgac caacactaat acaggcggta
    24181 agcctaaata tacttctgat aaatttccga tcggcataaa caaacccgtt aacgccagcc
    24241 aggaaatggc ttttacattt ttaagtttgt cgctaaaccg gagcaacaaa taaccaataa
    24301 gaatattcag aaaagcaaac aggttaccgt gtacatgtgc aagccgggct tcaaaatgtt
    24361 ttccgatgct ataagaattg gcccattcca cttttcccgg cgcaaagtca cggaggtaaa
    24421 tgagaagaaa tccatacgcc ataaagaatc ccatcgtcaa aaaaccgatt gcgatattgt
    24481 tttttccgtt catcgtcttt tgttattaaa agtgaaaaaa tgtggaaccc aaggtattgt
    24541 tgatcttttg tattaactat gacctgtgtc accttatggg tgaattgtgg taagtgatca
    24601 cgaagaatat tcgatcttct tttgtggtta tcggtctgtc agcaacaatt gcggcaggtg
    24661 actgaaccga ctgtccgctt gaagggtaca gttatggaac tgcaggagac agatgaaatc
    24721 gttgtgccaa agcggaagtc aattcatcct gctattttta aagcatggta ttccgaaccg
    24781 atcttaaatg atttatatta atgaagttta attaagtctt acttatcttt taaatttagg
    24841 tatattcaat tttatatttt actatgccca tcttcatgga tctccacatt gttcccggtg
    24901 ttaatgctaa ggatgtagca gaagcacata gcagggatgt gtatcttgaa aaggatcata
    24961 attgcaagtg ccttacttat tggattgatg agacccgggg tcatgtgttt tgtttgatag
    25021 atgcacccaa taaggaaact gtatatgaac tccataacag gtcacacgga ttgttaccgc
    25081 ataaaattat tgaagtacag aacgattttg tagaatcctt tctgggacgt attaccgacc
    25141 ctgaaagcgg gcaatatacg aataccggtt tgctgatgct tgatgatacc tcttaccgta
    25201 tcatcatgtc tgtaaataca acggattcaa ttttactgca tcatcaaatg ggtttggaag
    25261 gagctgtaaa aaaggtagag aagcagaata ctattataag ggatgctatt agaaagcacg
    25321 gaggtaaaga agcgctgcac gaaggccggg caattattgg ttcttttatt gcggcagaaa
    25381 atgcagtgaa ctgcggactg gctattcagg agcaaatgaa cgggctgcct gatgacagtt
    25441 tttctgtaca gatacatgcc ggtgagccgg tggcacaaac ggatgaattg tttggcgata
    25501 cccttcaact gttgcagtgg ctgggtatgc tgaaaagaaa agaggctatt ataattaccg
    25561 atggtataaa agaactcatt gcaaaagata tgcaggataa gcgcaccgta caactgctgg
    25621 cgcttacctt gccggacgag cagtttctta tttcattatt tgatgtgctg aataagaact
    25681 atgctgacaa tgaatttagt gcagagaact acgcacaaat acttacgatg agcaaatcgc
    25741 aaatgtaccg cagggtaacg gcacttaccg gttattcacc caacgacctg ctaaaagaat
    25801 accggcttga aaaagcaaaa gagttacttc gtaaaaagaa gcataatgta tttgaagtgg
    25861 ccctggaaac agggtttaca agtgcttctt acttcaccaa atgttttaaa acaaaatttg
    25921 gattattgcc attggcttat caacaattag tttagcccct tttggcctgc atcaatttta
    25981 ccattacttg aatgaattct tacaggtttt cattatttcc tgctattact ttggatcatt
    26041 attaacggtc taaactttta gcaatgaaac aaattttttt aaccagcctg ttgttattgg
    26101 cgctaaccat cttttctatg aacaatgcat ctgctcaaaa cagtaattct aacactgaaa
    26161 aaatccagcc ggcattaaaa acctatttga ttgaacggga tatacccggt gccggtcaat
    26221 taaccgctac cgatttaaaa gggatttctc aaaaatcctg tacggtatta aaagaaatgg
    26281 gatctggcat ttcgtgggtg cagagttttg taaccggtaa taagatcttt tgtgtgtacc
    26341 gtgcagaaaa tgaaacgctt ttgcgggagc atgcaaaaaa aggagggttt ccgattactt
    26401 ctattaccga aattaacaac acgatcagtc ctaaaacagc agagtaatcc tgcaattatt
    26461 cactaacctt cttctaaaat tgttgaaggg tgtcacgata aaacaatttc aaaacggccc
    26521 agtttttcat ctttctctgg cgcaattgag caatcaaaaa ttaaaatgta tgaaaaaagc
    26581 tatccttttt ttagtatcag ggtttctatt cgcatgtacg gcaagcagcc aggatgcaat
    26641 aaggaagtct gtgcccggtg caaagatcta tttttctgat aagccattta caacaagcaa
    26701 caccgggagt aaaagcagtt ttaactcatc tgattttatt tacggccgta ttgaactgga
    26761 taataaaaca ttacagtcgg cttttggttt gcctaaagat ggtgaatcac agatgtataa
    26821 taaaagtgat tgttatctcc gctaccaggt aacggtattt aaagatggag aacaaaacgg
    26881 gcagccaaac ttttgggatt ttttgtatgt ctggggcaat gaaaaaaata ataccgcttt
    26941 taattttgat atacttcctg agcctgctaa agcaaagagc atgttgtgtg gaactgaaaa
    27001 tttcagctct gttattgcat ccggcccgct ttatcatatt attaaccagg aaagatttcc
    27061 tgacaatggc acctacacta tccgtgtaag attatttcag caatctgcag atgcgtgggg
    27121 caatatggaa gcggaagaaa aatggccagt agtggaagaa gaattcagct tcaatttcga
    27181 tacgaaggat attaaacaat taaagaccaa tggtgatgcg gccggtgagt tggtgatgga
    27241 aaatgcgtac cggcttgata aaatgcccga atggttttcc aaagcaggca agcagaatga
    27301 ccctaaagta accaatgcag caatagcagc gatcttaaaa cgggacctcc ctaccaaatc
    27361 gatcattaag tttgttataa gtgaattcag tggccccacc tggcaggtgg aaaaagatga
    27421 atatggggtg attcaacaac gaacctttat gccaaccatc aatattgcgt acaaacggga
    27481 tggcaagtgt tatataggaa cagtacggct ttgggagaca tatgaaggat atggaaaata
    27541 cggcacgctg atcgtaggct ccgaatccag cagcaggcgt tctgattact ggctcgattg
    27601 tacgctaatt aaataaataa accttttaaa aatcaagcta ttctgtttta ttaatcaagg
    27661 tatgtgcaaa acggcctcgc tatttacctg aaattttctt tttcaatcaa catcctgcca
    27721 catacttatt caaggcaaac attcacacca aattactgac tcaaaatatt ctcattgtga
    27781 aaaaaaatta cacaacagtt tttatcgcat tcataatggc ttttaacagc catgcacaat
    27841 ttttttcgct tgcaaaggat atcaatactt cagcacttac tgaaagttct gctcccaatg
    27901 atatgattga aataggagga actgtatttt tttctgcata cacattcaat cgtggtcgtg
    27961 aattatggaa aagcgatggc accccgggag gcacagtggt agtgaaagat tttaatgccg
    28021 gcagcgctac cagtaatcca caatttttga ccaatgtaaa tggtgtttta tattttgttg
    28081 caagagatct tgttcatggg gaagagctgt ggaaaagtga tggtacaaca gctggcactg
    28141 tactggtgaa ggacagctat gccggcgacg ccaccagtaa cacccgcaac ctcacgaatg
    28201 taaatggtgt cctgtatttc gtggcagctg atggcacaaa tgggtttgaa ttatggaaaa
    28261 gcgatggtac ggaagccggc accatagtag tgaaggatat taacgccgga ggcttctcca
    28321 gtaacccgca atttctaacg aatgtaaatg gtgttttgta tttcaccgca acagacggta
    28381 ctaatggcgt agaattatgg aaaagcgatg gcacagaggc aggtaccgta atggtgaagg
    28441 atatttatgc cggaacttcc aacagcgcac cgggagcgtt aacgaataaa aatggtgtgt
    28501 tgtacttcag tgcaactgat ggcaccaatg ggcaggaact atggaaaagc aatggtacaa
    28561 tggctgggac tcaactggtg aaagatattt atgccggacc taaccacagt ttgcttttta
    28621 atatggtgaa tgtgaatgat gttttatatt tcagtgcaac aaacgctgcc aatggatctg
    28681 agctgtggaa aagcgatgga acggctgcag gcactgtatt agtgaaagat attaatcccg
    28741 ggagcaatac cagtgcactg caaaatttaa caagcgtaaa tgctgtttta tatttcacag
    28801 caaatgatgg taccaatgga atcgaattat ggaaaagtga tggtacagca gcaggcactg
    28861 taatgctgaa ggatatttat gccggctcta ccactagctc tcctcaatta ctgacgaatg
    28921 taaatggtgt tttatacttc agcgcaacag acccagacaa tggaacggaa ctttggaaaa
    28981 gcgatggtac agcaaccgga accttattgg tgaaagacat ttatgccaca gtcggtaatg
    29041 ccagtccgca ggcgctaaca aatggaaata gtgttttata cttcagcgca actgatgctg
    29101 cccatggcgc agaactatgg aaaagcgatg gtacggcggc gggcacccag ctcatcaagg
    29161 atatctgggg aggcacctat tacagctccc cccgaaacct aacggatgta aacggcacat
    29221 tatatttcag tgcttttgaa cctgccaatg ggcaggagct ttggaaaagc gatggtacgc
    29281 aggccggtac gatgctgcta aaagatctta accccggaag cagttccagc actccgcaat
    29341 ggcttacagc tttaaatgga aatttatttt tcactgccac cgatggaagc aatgggctgg
    29401 tactatggaa aagtgacggt actacagccg gaacggaatt actcagagaa gtggcagcca
    29461 accttcctat tcgttcccct cgatatctca ccgttgtaaa caatgtgttt tatttcagtg
    29521 caatcgatta tgcatacggg caagaactat ggaaaagcga tggtacgacg gcaggcacca
    29581 gaattgtaaa agatataaat atgaccggta acgccgatcc ggaaaacctt accaatgtaa
    29641 atggcacgat gtatttcacc gctgtgagta gcagctttgg tcgggaactg tggaaaagcg
    29701 atggaacatg ggccggcacc gtattggtaa aagatatcta tccagggcca atcggtggca
    29761 cgaaacggta cttcacgaat gtgaatggta cgctttattt cagtgcagat gatggagtca
    29821 atggagaaga actttggaaa agtgatggaa catctgccgg caccataatg gtgaaagata
    29881 tccttcccgg tgaaattgga agtagtccgc aaaacctcac gaatgtgaat ggtgttttgt
    29941 acttcagtgc aactgatggt atcaatgggc aggaactttg gaaaagcgat ggcacggagg
    30001 ctgggacggt tttagtgaaa gatattaatc cgggaggcat tggaagtgcc ccgaataccc
    30061 ttacgaatct aaacggtgtt ttgcattttg ctgccgctga tgctgcgaac gggtgggaac
    30121 tatggaaaag tgacggtaca actttaggca cagtactggt taaggatatc tttccgggaa
    30181 ttaattcgag caacccggta agtttaactg ttgtcaacaa tacattattc tttacggcaa
    30241 acaacggcac cactggccag gaagtatgga aaacggacgg tacagaagca ggcactgtaa
    30301 tcgtgcagga tattaccgtg caaggagcct ctaatcccaa tagctatacc ttggtaggtt
    30361 ctaaattatt tgctgctatt accgacgatg tacacggtga agaactttgg gtggcacaaa
    30421 ctgtaaccgt gctgccatta acgttactgg agtttaatgg caggcttgta aataatgatg
    30481 ggcttctaaa ctggaaaact accgatgagc aaagcacagt ttcgtttgac atcgaacgaa
    30541 gcaccgatgg aagaagctac atttctgtcg gcaacgtttt agcagctaat gttaccggca
    30601 ttcaccacta tcattacaaa gatgataacg ttactgcttt gggtgtttca aaagtatatt
    30661 atcgcctcaa acaaaaagat atcgacgaac gctattcgta ttcaggaatt gttgtgttgt
    30721 caattgataa tagcaggagc aacgtgttac tttatcctaa tcctgtcagt agtaaagcga
    30781 atgttactat cactcttaca aagccggaaa aaatacaggc aagaattatt gacaatacgg
    30841 gtcgtatcgt aaaactgcaa caattcaatt tgtcggccgg cagcaattca attgcaatag
    30901 accttggtca aatatcaaaa gggatgtatt acctcgagtt gaagggagaa actattaatg
    30961 aacgaaagcc ttttgtaaaa caatagctgg ttagggcgac cactaacgat cagggattac
    31021 tgcaggttaa gcattagtat tccgccaggc tgtaattgct gttaaccgga aaaatagttc
    31081 cggattttcg ttgtcttagt attgtaaatg ataattatgc tgtttatttc ttttttggtt
    31141 tcaccagccg cagttgccag gaaggttttt ccatttctta aacgcagcca tactgtgccg
    31201 ttggtttata atagtttgaa gcctgctctt ttatgacagc atatctgtta ttaagctgct
    31261 aaacattttg aatagttaat cttctttttt cttttaatgc tttaaaatgc gatggggtca
    31321 acccggttac ttttttaaac tgcgttgata aatgggcagc gctactatag tgtagtttat
    31381 gtgcgatttc ggaaaaatta tcttctccaa aaattattaa ctcctttatc cgctcaattt
    31441 ttaaggcgat aacagattgt tcaatggtgg tggcttccac ttccgaaaag aaattggcta
    31501 agtaagtata actatgattt aattttttac ttaacaacac agagaattta atagttgact
    31561 tttcgtctga attgtaaaca taatcaacta tgactttcct gattttctca attaacatac
    31621 cgtttttttt tcagtaactc taatcctgct ttctttattt tgctgttcag tttcattttt
    31681 tcctcatcag aaacatcgtt cctggtttct atttccccta attcaacttt aactgacgga
    31741 atgtcaagtt cttctaaagc ctctttcact acaactttgc aactttcgca aaacatattt
    31801 tttatataga ttttcataaa ttatttcttt tacttattaa acacaagtcc ccgcatctta
    31861 cgatcgggca acgtttgctt tgtccgccaa taacactaat gtatctgttt aatttggttt
    31921 cttgctttaa tgttctggca aacaccccgc caattggttg cagctaactt acatagcttt
    31981 ccgaagtaag ggaattggta atccatctgc ccggtactga tgatgacaga aacgccgaaa
    32041 ttgcagcaga tatgcttagt atttgtatat tatccgctct aaataagatc aaccgtaaga
    32101 cagaaaagtt aatttgatga ggcacctgtt ctaattggac aattcatcca tcaattgctt
    32161 caatttcttt aaatgtttgc cataagcata gttgaacatc caccttgccc cataatagca
    32221 taaaggaact aaaattaaaa gcgtaacacc gagcacaagc aacatttttg gattgtacaa
    32281 aaacacctct accggtttac ccgaaccttc aacaccaccc agtatgaaac caccagttgc
    32341 tgctattgga taaacaaata gacctaatct ttcctgtata cgccaccatt tttgaaaacc
    32401 cgaatagttt ttttgcaact cgtttttcaa agagtttgtt gcggatatgt ttgcattgat
    32461 gtttgtatac aatttccagg tatcaagccc tacccaaata ttgaagagta ttgcaataac
    32521 tatagccaga tgaacctgcc aaatgctgat aaagaaaatt aatactacat aaatagcagt
    32581 aataagaata gccaatatta aaccgattaa aagattcttc tttaatttgg ctaaaggtaa
    32641 tttggagtgc aggttactga attcatttgt ctgcaacatt ttatttagtg catcatcgct
    32701 cccggcactt tgctgccatg tgttttcgat gttcatctat atatttttta aaagcgttgc
    32761 taattgttgt tttatccggt gaagctttac acccaccagg tttgcggtaa tccccatcca
    32821 ttcaccaact tccttattat catatccatc caaatgaagg gaaatgatgg ccctttccgt
    32881 ctctggcagt gttcttatcg ccctgtataa tctttccgct tcatcaatgg caggtgtggt
    32941 ggaatacact tcctcaaact gagccgtatc agtgtattca accctgttcg tttttcgttg
    33001 aatagtaaaa atcgtattca ggcataggcg gtataaccag gtgctgaatt ttgcttctcc
    33061 cctgaaggca ggataggttt tccatgcctg caacaatatc tcctggtaca aatctttttt
    33121 atcttcttcc gtatttgcat aaagatgcac cagcttataa attatattct ggtgctcttt
    33181 gagttgtgat aagaattctt tttcgctcac ttatttttaa ggtttacggg tgttacagtg
    33241 tatccctgct cttttaattg attcaccaac ccgctattgc ccactaaatg taaagcaccc
    33301 actgcaacaa acaaagattg atctttcatg agtccgggca gctgttgcat ccagtagtta
    33361 ttgcggtcat ccaataaaat cttcatttct tccggcctgt aactgctgcc atacattaat
    33421 tcctgcagtt ggtttaagtt ttgagaaatg taagcattgt tcattatccc aataagccgc
    33481 ctcagcgcat ctttatcttt gaatgtttca tctaacagct caacctggcg ctgcaacgtc
    33541 agttgttcaa acatggcttt aatctgcatt tgtatcgttt ccagacccaa taccttctta
    33601 ccctctttct ttgcttcctc ctgaaaataa gtgtctaact gaacttctcc cggctggtta
    33661 ggaaaatatt ttccttttgt tatggccata gctgctgtaa gcactgttac agggttcagc
    33721 tggtttaact ggccgatgtc taaacctgcc tcttgcttaa accaatcaga tgcttttgca
    33781 tacaatgtat ccggcaacac ttgctgtaaa gtagtacctt ttaacacagc cgcctccatc
    33841 atggggcgtt gcagggaagt atcaattacc agctctccca ctaccgcatc tgctgactta
    33901 taagcggttt taactgtttc cagcgtatcc acaaaaccac ttgacaggaa gtggtaagtt
    33961 ccaaaaagat aagacggttt ctttaaccca ttgccggtta ctttccagag cagggcgttt
    34021 tctgttttgg attgttgtgc tattataact gctggccaca acaggcataa tgctgcctgt
    34081 agaaaatttc ttttcatttt ttaatttttg aagtgagtat ttaatgctat aaaaagatga
    34141 tataaaacaa accagagaaa gtgagggcta aatgaaccag cagccatact ttttgataaa
    34201 tgggtctttg ctgccattta acagaggggt tgaacgggtc gaacaagagt gcaattccca
    34261 tgttggaaaa tgcccattcg taatcgcctt taataagaag gtagataacg agtaccatgt
    34321 aaacaacgta agcatatcgg ttaaatggtg taaattttgg ggcagtttcg gaggatgatt
    34381 tcagtttcat aacctgctat tttatatata agtggcacaa gggcttcctt tattacagaa
    34441 aatgaaatta ttttttctgc tttttcggga tagttgctaa agtcatagct aaggcgaagc
    34501 tgattagaat tctgacggct ggggtgcggg gatttcggtc tgatttttag cactttgtcc
    34561 caaaagcacc cgggattggt tttgtgtcgg gggtcttttg cggtttgtca aaatagcatc
    34621 gtcccgtgtc ggtaaaatcc tggattattg aataaacgca ggatattcaa aagggtgatg
    34681 aacaacggta cggcttactg atggacggga gtttggaaac gttcacagtc ttgccggaga
    34741 ttgaatttac tttgaaaaag gaacatcacg attaccgtct tacctggtta gcggtaaact
    34801 gctgttacag ggcgtttttt agctgtaact attgtgtgta tgtcaaattc ggtttctgat
    34861 actttgtgct taaccttaaa ggtttcgatt tcgtcaaaat ttgtcccgac aagctgctct
    34921 ttaatgtccc ccaaattatg aaaatgaaag aacaccctac ccgcaccact aactttaaaa
    34981 tcagacttgg caggatcacc ttctacgaaa cttaaatata tcaagccgtt atcgtttaat
    35041 aattcgtaag aatgagaaat cagttcttta ctttcagttt tagacaaata gggcaagcaa
    35101 aacccaccga taattccgtc atactttttt tcaagactgt taatttgccg gctgtccata
    35161 acagcaaatt ttgctgtcgg attattcttt tcagcaagtt caatcatatt gggggaaatg
    35221 tcaatcccga aaatatcaaa atcgggtctt tgggataaaa ggtatttagt gatgtttcct
    35281 ggtccgcaac caatttctaa tacttttgca ttaaccctgg taattgaatt gcaaacaaaa
    35341 tcatacgttt cattatacaa gtccaggtcc ataaacttgt cttgatatag tgaagcaagt
    35401 ttgttccagg tttcaaatgt ttctttatat ctgtccattg tcgtgtcgtt tcctaaaagg
    35461 cagcatacgt ccagggcttt atgcaggttg ggaattcatt gattgtccag cccgaaccgc
    35521 tgaggcgaca ttgctgatgc taaagagttg atagtcctgc
//
LOCUS       DAORTI010000019        35480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_751_length_35480_cov_7.189640, whole genome shotgun sequence.
ACCESSION   DAORTI010000019 DAORTI010000000
VERSION     DAORTI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 35480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35480
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_35480_cov_7.189640"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PLO70_04435"
     CDS             <1..1618
                     /locus_tag="PLO70_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73734.1"
                     /translation="TLKFGSGLVNGFTTDIRLSSISSDGFIDRAKSSLKSFHFSTAYI
                     GKTTSLRLNVFSGKEKTYQAWYGIAEADLKAGNRTINYAGTEKPGEPYDNESDNYTQT
                     HYQFFLNKSIGKKLNLNTALFLTKGKGYYEQYKAEEDYSAYRMPYPVNGPDTIFSSDF
                     IRQLWLDNYFYGTIYSLHYKAAKTELTIGGGLTTYDGNHFGKLTWASNGLTSLKNWYD
                     NDALKTDFNIYLKQQTQLNNYWNLFYDIQFRYVNYKIEGFRNNPGLIVNNKFSFFNPK
                     AGIVYKKDSWKSYLSYSVANKEPNRDDFEAGNTQQPKPERLQDFELGIEKSTKQLNWS
                     ATLYYMKYKDQLVLTGKINDVGAYTRTNIPESFRAGIELQGGVKFNSWINTTANLTLS
                     RNKVIDFEEYIDDYDNGGQKINSFSSADIAFSPSITGAGSINVLPFKNTEISFISKYV
                     SKQFLDNTGNNSRSLNSFYVQDIRAIYTIKKKWLKDVTITGQVNNLFNKEYEPNGYTY
                     SYIWGGETITENYYFPMSGTNFIIAVNIRL"
     gene            complement(1670..1984)
                     /locus_tag="PLO70_04440"
     CDS             complement(1670..1984)
                     /locus_tag="PLO70_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ73735.1"
                     /translation="MDIITVEELKARIDAGEKLNLIDCREPFEYAEFNIGAKLIPLGD
                     IQRMQIEEIEDLKEEEIIIHCRSGQRSMMACMFLDTLGFKNTKNLIGGVLAWQEKFGS
                     GK"
     gene            complement(1997..2803)
                     /locus_tag="PLO70_04445"
     CDS             complement(1997..2803)
                     /locus_tag="PLO70_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQZ73736.1"
                     /translation="MFKFITHRPLWLNVIIGILLSIGIFALFLLSLNWLTGHGEAATV
                     PSVAGKSYEEARSILRKAGFDVEVQDSVYVDSIPPLKVIRQIPDADEVVKSNRTVYLI
                     IRRAVPPLVEMPNLIGYSYRNAEMVLRSMDLRVGDTTFKPDFAKNAILEQWYEGAMIT
                     PGTKIRKGSAISLVMGDGIGKMEFVVPVITGMTYGDAKSLLERAGVGIGAIVLDANVS
                     DTISAWIYKQSPERLDDEKRIQRIRSGQLIDVWLQADKPVADSSKNTTEI"
     gene            complement(2834..3826)
                     /locus_tag="PLO70_04450"
     CDS             complement(2834..3826)
                     /locus_tag="PLO70_04450"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQZ73737.1"
                     /translation="MKKNIALVTGGFSGEAVISYKSAVTIDNNLDRNLFNVYKIDINS
                     QGWFYELTDGRKLEVNKNDFSLQLDNEKVTFDAVFIGMHGTPGEDGKLQGYFDTLKIP
                     YTGCDAATSAITFNKRYATAVAGAAGVPVAKSVFLIKNNFGRPDDVVANLRFPVFVKP
                     NNGGSSIGMSKVDTPSDELNHAIEKAFKEDNQVLVEEMIEGREFTVGVFKSGGNIIVL
                     PLTEVKAHADMAFFDFEAKYQGKSTETTPAEVNEAIADKIRDTARKVYSVFNCRGVVR
                     IDFIYNEKEGSPYMLEVNTIPGQSEASIIPQQVRSMGWSLKEFYTKLVQECFYS"
     gene            3898..4086
                     /locus_tag="PLO70_04455"
     CDS             3898..4086
                     /locus_tag="PLO70_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73738.1"
                     /translation="MPNLKSSYQIALTKNIIKPSLLKTDLGDYHSCHAPFLGVENKKG
                     EGRFSFTFLRRDISPATH"
     gene            complement(4193..4540)
                     /locus_tag="PLO70_04460"
     CDS             complement(4193..4540)
                     /locus_tag="PLO70_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQZ73739.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAVADGTTVKGSYT
                     LLDGTVVDAEQKNAPVSVDTYYITPNKCTECQGFHEEPQCAAVCPVDCCVPDDMYQET
                     VEQLMAKKDKLHI"
     gene            4598..5596
                     /locus_tag="PLO70_04465"
     CDS             4598..5596
                     /locus_tag="PLO70_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQZ73740.1"
                     /translation="MNLQHRIYLLSRLGEYILSTEVEWQKAKEKANWENGWFIPDFVE
                     LASKNIALSFLQKDILEKWAGGHQIPASSTNSRTIGIVMAGNIPLVGFHDWLSVFISG
                     HKAIIKLSSKDQVLFKHLIEKLAEWDPLVNKFTSFSEMLKGCDAYIATGSNNSSRYFE
                     YYFRKYPTIIRKNRTSIGILSGKETNEELRKLADDVYLYFGLGCRNVTKIYVPSGYDF
                     IPLLDAFNKYRHLADHNKYKNNYDYNLALHLLNKKYYMTNGSILLVEETAFFSPISQL
                     NYEYYSDTDKLSDSLKNHPELQSITGEGYVPFGQSQYPAISDYADGVNTLKFLSTL"
     gene            5789..7291
                     /locus_tag="PLO70_04470"
     CDS             5789..7291
                     /locus_tag="PLO70_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQZ73741.1"
                     /translation="MKLKQLFLIIAVSAVSAVSSVWIYSKVAAKQQSSIFSQSNEGKV
                     PVNYAGFFDNAASTVGEVTDFTKAANAAVPAVVHIKTKIPAKKVTNQLPRNRGNSMDD
                     FFEQFFNFGPQIQPEQRASGSGVIISDDGYIITNNHVISDGGEGVADEITVTLSNKKT
                     YKAKVIGKDPSSDIAVLKIDGTGFPFLLYGNSDNVKLGQWVLAIGYPLTLETTVTAGI
                     VSAKGRSIGINGRQSQTPIESFIQTDAAVNQGNSGGALITTDGQLIGINSAIYAPTGT
                     YAGYSFAIPVNLAKKIVNDLIKFGDVKRPYLGIAADQTKVNSTDGAYIGTVAKDGAAA
                     AAGLKKGDIIKKINDVTISSWSELQGTVASLNTGEKVNITYKRDGKEYTTSATLKAET
                     GTYDNLAASNLGDVLGAELENLDKKTAEKNEVEGGVVVKKIKPGGPFSKTRMQDGFII
                     TSVNGVDITNTEELSRAIASLRSGSLNLEGFYDGYDGMYRYPVNLQLEDE"
     gene            complement(7370..8755)
                     /locus_tag="PLO70_04475"
     CDS             complement(7370..8755)
                     /locus_tag="PLO70_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQZ73742.1"
                     /translation="MRFFWLALSLTAILGSCKLMKKKEDSETKKSNNMTGRFKTLDWV
                     HSTNIYEVNIRQYTIEGTFNAFAREMPRLKDMGVETLWFMPITPIAQKNKKGSLGSYY
                     ACSDYTSINSEFGTLDDFKNLVKQAHELGFKVIIDWVANHTGWDHKWTIEHPDYYLKD
                     SATNDFKIASGMEDIIELDFKNPDLRKAMIDAMKFWVTECDIDGFRCDLAFWVELDFW
                     KEARPQLDAVKPLFWLGEFDELEKPEYGEAFDASYTWTWMHKTEEFYKQQLSIDTLDV
                     ILKKYDDLGDSTMRAWFTTNHDENSWNGTEYEKYGDMAKALAVFSATWNGVPLLYSGQ
                     ELPNQKRIEFFDKDTISWTGNNLLHDFYKTLLHLKTNNPALRGGDTTVKTFHIKTTDP
                     QKIVAYIRKSGDREVLVLLNLSSQNNLHFDVIDERLTGTFKNVFSGAANDFTVEKGFE
                     MQPWEYLVYEK"
     gene            8845..10056
                     /locus_tag="PLO70_04480"
     CDS             8845..10056
                     /locus_tag="PLO70_04480"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQZ73743.1"
                     /translation="MKSFEVPIIYRSPLISAIKKKRKETDKMKKDFSPSLLDFGPIHI
                     YLARHFGFCYGVENAIEIAFRTVEENPGKKIFLLSEMIHNPQVNTDLLAHGIQFLQDT
                     YGKQLIPFEEISADDIVLIPAFGTTLAIEKKLQHIGIQTEKYNTTCPFVEKVWNRGEA
                     IAKNKYTIVIHGKPKHEETRATFSHASANAPSIVIKNMNEAVELGKYITGEKSAETFY
                     TEFEGQYSQGFDATKDLQRIGVINQTTMLASDTQAIADYLKKIIIGTFNSDDSFADTR
                     DTLCYATNDNQTAVSGMLETKADLAIVVGGYNSSNTSHLVELCEEKLPTYFITNEEKI
                     LSPREILHYNFHTKNELLTTEYLPSKEPVKVLITSGASCPDALVEGVIHKLIQFFPVS
                     KSMDQLREEFQ"
     gene            complement(10106..11761)
                     /gene="glgP"
                     /locus_tag="PLO70_04485"
     CDS             complement(10106..11761)
                     /gene="glgP"
                     /locus_tag="PLO70_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQZ73744.1"
                     /translation="MSFTKFSVPYPVDERYSKRVAYFSMEFATHQPLKIYSGGLGFLA
                     GSHLRSAYELRQNLIGVGILWKYGYYDQERNQDQTLDVAWNEKHYSFLEDTGIKFQIT
                     IHEHSVWVKALYLNPETFKTAPLFLLSTDLPENDYVSQTITHRLYDANVATKVAQFIL
                     LGVGGAKLVDLLGFNPELYHLNEAHGLSAAFYLYKKFSNNIQEVKKRLVFTTHTPEEA
                     GNEKHDIYLCHKMSYFCGLSVEEVKKLYDNDSDQFNHSLAALRFARLANGVSQLHGRV
                     SRAMWEKYENICPIISITNAQNWRYWADKQLYRFMEAGDDYGIDDRKKYLKKRAFEIV
                     ADQTGKIFDPEVFTIVWARRFAGYKRAGLITTAEERFEKLINSKKYPVQIIWAGKPYP
                     VDHPAISEFNQLVHLSKGYKNVAVLIGYELGLSKRMKQAADCWLNNPRVPREASGTSG
                     MTAAMNGAVNFSTDDGWIPEFINHGHNGFVVPMADYESMAVHEQDQYDLNKLYEILEN
                     EMLPLYYENYSTWRQVMKNGMQDVRFQFDSNRMAHEYYDLLYR"
     gene            complement(11897..12895)
                     /locus_tag="PLO70_04490"
     CDS             complement(11897..12895)
                     /locus_tag="PLO70_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73745.1"
                     /translation="MAVLKQFLLLLLGALLLAGCKPKKKPILSGEDPVEVSDFIEFFQ
                     PLSLSYQVGDTILDKKDKDSLLISYKIFTEFVPDSVLRKVFGKGIKPKIYAIGKAAVP
                     KAETYLLVKTVTPAKKVLFILAFDKKEQYLASIPVLVPDQKKATTQSVTIDRKYTITK
                     TVQRKNPDASISEGKDVYVLNADARNFMLIMTEALEDKVTELINPIDTLPRKNKYSAD
                     YSSGKMNLVSVRDGRKSDRITFFIHIEKNNGECTGELKGEARWISSNLAEYRQDGDPC
                     VMQFKFTSNSVSLKEEGCGSRRGLNCSFDGSFARKKYVKPIQSNNKQAASKKSDKK"
     gene            13002..13667
                     /locus_tag="PLO70_04495"
     CDS             13002..13667
                     /locus_tag="PLO70_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQZ73746.1"
                     /translation="MLNYTTSEENYIKTILHLQKQDGTVTTNELANELKTKPASVTDM
                     MKKLKAKKLVNYQPYQGFRLNSEGTRVALGIIRRHRLWEYFLAEKLKFSWDEVHEVAE
                     DLEHVSNKKLIDKLDEFLGFPRTDPHGDPIPDASGKIEISKKICLTELKLSSPAVVIS
                     VSDQSSEILELLEHKKIAIGTRLEIKKRFEFDNSMEIRIGRQPVFTISEQLAKNIFVQ
                     SNS"
     gene            13671..14495
                     /locus_tag="PLO70_04500"
     CDS             13671..14495
                     /locus_tag="PLO70_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQZ73747.1"
                     /translation="MEAIEKFFIEIGPVWSALLATTFTWFVTALGASLVFFFKTLSRG
                     VLDPMLGFTGGVMVAASFWSLLNPAIEISEKLYPSMSWMPAAVGFLLGALFIFFLDKV
                     TPHLHLNFGVEETEGVRTKLHKTTLLILAITLHNIPEGLAVGVLFGAAAIGMEDASIS
                     GAITLAIGIGIQNFPEGIAVSMPLRRHGVTRLKSFWYGQLSAIVEPIAGVVGAMAVIY
                     MQPVLPFALAFAAGAMIFVVVEEVIPETQRDKYTDRSVLGFIGGFLVMMILDVALG"
     gene            complement(14499..15098)
                     /locus_tag="PLO70_04505"
     CDS             complement(14499..15098)
                     /locus_tag="PLO70_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ73748.1"
                     /translation="MDSIKAFYNYLQRFTDLTEDEFNRNLLPICKVRRFGKKEIITKA
                     GEIENYFNFIVKGLARKYYKKNHHEINTHISFEGQMLLSQESFHSRQPSDYFIETIEP
                     TTVVSISYDDAEKMFASSQRMEHLGRLIVTYSMVIKDRWQIQLVKMTPRERFLNFVTK
                     NPELMQRVPQKFLASYLNIKPETFSRFKHLIRTHKVSAN"
     gene            15287..16519
                     /locus_tag="PLO70_04510"
     CDS             15287..16519
                     /locus_tag="PLO70_04510"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HQZ73749.1"
                     /translation="MTQKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLEVDIKYYD
                     LGIEYRDQTDDQVTMDAANAIKEYGVGIKCATITPDEARVKEFNLKQMWKSPNGTIRN
                     ILDGTVFREPIVISNVPRLVTNWTAPIIVGRHAFGDQYRATDTVIKGKGKLTMTFTPE
                     GGGEPQTFEVFNFKGDGVAMSMYNTDESIKGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFEEIFQNEFKASFDAAGIVYEHRLIDDMVASALKWNGNFVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLITPDGKTMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIF
                     AWTRGLAFRGKLDSNQPLIDFCNTLEKVCIETVEEGKMTKDLAVCIHGNKVKHGDHFL
                     YTEEFLTAIDENLKKKLK"
     gene            16674..17171
                     /locus_tag="PLO70_04515"
     CDS             16674..17171
                     /locus_tag="PLO70_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQZ73750.1"
                     /translation="MKKRQDYISWDEYFMGVALLSAKRSKDPSTQVGACIVNQKNKIV
                     GAGYNGLPIGCSDDEFSWDKKGEFLDTKYPYICHAELNAILNNIGMDLQGCKIYTALF
                     PCNECSKAIIQSGITEVIYLSDKYEQDDIFKASRIMLDKAGITCRKVETRIMSLELSF
                     RETDV"
     gene            complement(17256..17723)
                     /gene="rpsG"
                     /locus_tag="PLO70_04520"
     CDS             complement(17256..17723)
                     /gene="rpsG"
                     /locus_tag="PLO70_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQZ73751.1"
                     /translation="MRKAQAKKLPLAPDPRFNDKLVTRFVNNLMWEGKKSGAFIIFYD
                     ALDKVAKQTNEDGYEVWKKALANVSPAVEVRSRRIGGATFQIPSEVRPDRKISLSIKW
                     LIRYSRDRNGRSMADKLAGEIVAASKGEGAAFKKKEDTHRMAEANKAFAHFRV"
     gene            complement(17751..18131)
                     /gene="rpsL"
                     /locus_tag="PLO70_04525"
     CDS             complement(17751..18131)
                     /gene="rpsL"
                     /locus_tag="PLO70_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQZ73752.1"
                     /translation="MPTIQQLVRKGREIIRAKSKSRALDQCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVKDRKQSRSKYGTKKEKAKK"
     gene            complement(18292..19356)
                     /locus_tag="PLO70_04530"
     CDS             complement(18292..19356)
                     /locus_tag="PLO70_04530"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQZ73753.1"
                     /translation="MVAAMDIAITKAERSKLQDLNLDNLPFGKYFTDHMLEADFENGE
                     WKNAEIKPYQPLLLSPSTAALHYGQAIFEGIKAYKDKEGNAYIFRPHDNFKRFNISAD
                     RMQMPAVPEELFMEGMLQLVGLDKNWIPSKEDHSLYIRPFMFSSDELIGVRPSDNYKF
                     LIIMSPSGPYYSAPMRIYVEEKYVRAVTGGVGFAKAAGNYGAAMYATAEAKKKGYDQV
                     LWMDPFEHKYVQECGTMNVFFIIGNTAITPGLEQGTILGGVTRQSVITLLQELGLKVE
                     ERQLSIDEIIAAYKAGELKEVFGTGTAATISPIKELGYKDFSMKFDVDSWKTAPAIKK
                     WLTDIREGRAEDKLGWMQPV"
     gene            19369..19974
                     /locus_tag="PLO70_04535"
     CDS             19369..19974
                     /locus_tag="PLO70_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73754.1"
                     /translation="MKGQRNEKKLTPISTIINNLMDLNHIKLPATVIADLYKSSLVER
                     EENLTKKEAPATLPKQEIRPANTEIEVKSWKSLGNNLKHIMVIVNKTDVIYLPDNELI
                     FLTGILAACKLNISDVAIFNLGNHPGTTYKDLVSYFKSKNVFLFGVEPVDFGLPMNFP
                     HFQQQKFTGITFLFSPSLNELENDKLLKSKLWICLKQIFNI"
     gene            19989..20564
                     /gene="rdgB"
                     /locus_tag="PLO70_04540"
     CDS             19989..20564
                     /gene="rdgB"
                     /locus_tag="PLO70_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQZ73755.1"
                     /translation="MKLIFATNNQHKIDEIRSVLGKKIEIITLKEAGINIDIPEPYKT
                     LEENASGKSKTIFDITATNCFSEDTGLEVNELNGEPGVKSARYAGEGRSFDANIEKLL
                     SNLSGKSDRTARFRTVISLLIDGTETLFEGICEGRIIEKKRGLQGFGYDPVFIPTGSD
                     KTFAEMDIVEKNRFSHRKKATEKLVAFLNTI"
     gene            20579..21154
                     /locus_tag="PLO70_04545"
     CDS             20579..21154
                     /locus_tag="PLO70_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ73756.1"
                     /translation="MTPERNQNISENDLINGCMEDNRRMQEEMYRRFSPRMYAVCLRY
                     AGNAEEAEDILQEGFIKVFKKLDSFRREGSFEGWVRRIFVNTAIEHFRRKRYLMPVTE
                     KEENIIEGKYTSVLDELGAKDIMALIQKLSPGYRTVFNMYVVEGYTHKEIAEMLGISE
                     GTSKSQLSRAKVILQDMVRTYIDTQRGISTK"
     gene            21154..22068
                     /locus_tag="PLO70_04550"
     CDS             21154..22068
                     /locus_tag="PLO70_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73757.1"
                     /translation="MNRSLRDKMYNYEATPPAGVWNGIEAELNEAMTTNKLSAKMYGI
                     ELAPPAMAWNNIAAQLDETTLSNKLYNAEAIPPRAVWDKIESLLILNDDTRVNQKRKL
                     YPALRYAAAAIVIGFVVFGAVRLAQPNKSEGDVVMKETVIPENYNTEASATKDEIHKL
                     INNIIADNDEARNDAALEASKKTYARLDIPSRSIAKEVSNFYFASNNILTPGTRGLDI
                     EDTAPQANSPTDRYITLMTPEGNIIRMSKKLGDLVCCISGETDDPQCKDQLKKWREKI
                     VASPLGHSSESFMDILNLINSLQDNNYQ"
     gene            22104..22535
                     /locus_tag="PLO70_04555"
     CDS             22104..22535
                     /locus_tag="PLO70_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQZ73758.1"
                     /translation="MARIKIDIPVSFSFSCEIPVRITDINYGGHVGNDTVLTIIHEAR
                     MQFLGNSNYTEMNFAGAGLIMADAGIEFKSELFYGDVVRVFVTAGEVSKIGFDLYYKL
                     EKLVDDKKVLVAVAKTGMICYDYSKKKIIPVPDKAREILNR"
     gene            complement(22537..23268)
                     /gene="aroE"
                     /locus_tag="PLO70_04560"
     CDS             complement(22537..23268)
                     /gene="aroE"
                     /locus_tag="PLO70_04560"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631242.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQZ73759.1"
                     /translation="MRRFGLIGYPLSHSFSKKYFTEKFEQEEITECQYDLFPLKTIDE
                     LPFLLREYPELEGLNVTIPYKKQVFSFLDETNIPRGLDACNCINITNGKLVGYNTDIT
                     GFEKSLRPLLRSYHKKALVLGNGGAAAAVVFVLQRLGIDFTIVSRKIHEGAAITYKEI
                     NAEIIIDNTVIINTTPLGMYPDTDTCPEIPYQFISDRHLLYDLVYNPAMTLFLRKGEA
                     RGAAVKTGEEMLLLQAEESWRIWNS"
     gene            complement(23268..24041)
                     /locus_tag="PLO70_04565"
     CDS             complement(23268..24041)
                     /locus_tag="PLO70_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HQZ73760.1"
                     /translation="MNFNLSQIPDRKQKPRQSGLTMVMDKGLSIEEAKNFLSVSHPHV
                     DIVKLGFGTSFVTPNLREKLEVYRSFNVPVYFGGTLFEAFLIRNQFDDYISVCKDYGI
                     SYMEVSDGSITIPHAEKCGYIEKLTKHGTVLSEVGSKDAAHIIPPYKWIELMKAELEA
                     GSAYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPAEKIIWEAPQKAQQLYFIELV
                     GCNANLGNIAPAEVIPLEAMRIGLRGDTFNLYLDKKETA"
     gene            complement(24142..25563)
                     /locus_tag="PLO70_04570"
     CDS             complement(24142..25563)
                     /locus_tag="PLO70_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73761.1"
                     /translation="MQENPYREDRELIQELLKQFENLKTGRSHSFIEEDAFEKIIDYY
                     QEKEDLIRAMEAAEIASEQFPYSAMLLIKKADILLANRRYQEALDTLDQASLLDSGDI
                     NLYILKTDAFLALDQQQKAVKLLEEALSHFEGEEKIELLFELADVYDDYEDFDKVFDC
                     LKLILDDDPNNEEALYKICFWTDFTGRNEEGIRLHLKIIDDFPYNELAWFNLAAAYQG
                     LKLYEKAIDAYKYAITIEEKFDYAYRNMGDAYIRLRKYKEAIEALEKVNELAKPEDVI
                     FEAIGYCYEKMRNHAQARFYYRKASHLRQDDSKLFYKIACTYYNEGQWQACIKQLDTA
                     MKIHRLQPEYNLLAGECKMQLGLFRDAIQYFSTVVRIRPKNAGGWEALIRCLYKADFL
                     EEALLQAEAALHVTNGKPLFIFYKAAVLFAMGKTKEALLQLDSAMVNTPKLLKKFVEL
                     NPAILQNQQVVDMLARFKRNKSI"
     gene            25826..26197
                     /locus_tag="PLO70_04575"
     CDS             25826..26197
                     /locus_tag="PLO70_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73762.1"
                     /translation="MNLSKLTILWKNNSRKLAAIILLACSGMGAFATLGDGNKKSNLP
                     KKSLLSNKTVLKPGSFSLRSGYNYRGSNVINTENEKKYISLNTIVTVQKGKTTFIVPL
                     KKKVILNNLKIDIGNRQLQRN"
     gene            complement(26198..26860)
                     /locus_tag="PLO70_04580"
     CDS             complement(26198..26860)
                     /locus_tag="PLO70_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73763.1"
                     /translation="MKKIYIVFIAVFLLVVSFEMQAQRRLTEATISYDIVINTSNKTP
                     QAADLLDGATSVIYLKGALSRSDMVSSLGTQATIVDGKTGDVTVLKEYGDQKYMIRMT
                     QENWKESNKKYEGIAFTYAEDYKTIAGYKCQKAIGKLTDGSTFNVYFTKDLVPVNKDF
                     QYLNKNLPGLAMQYEATLGSMLVTYTVSSINFNPVQQAKFDLPKSGFRIMTYEESKGG
                     RN"
     gene            complement(26983..28842)
                     /gene="yidC"
                     /locus_tag="PLO70_04585"
     CDS             complement(26983..28842)
                     /gene="yidC"
                     /locus_tag="PLO70_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQZ73764.1"
                     /translation="MKFDRNTVIGFVILAALFFGYFYYTNQEQSAYRKQKAKEEFVKD
                     SIAKANIPPEDKLAQKIDSARADTMKRIASAGSFRDAANGTEQLVYTENEYLKLTFTS
                     KGGQVKRVELKDFKGQDSNLLQLAATDFDRLGYTINTATNQTAQTADLYFTGIDSAKN
                     ADGSKSIWFILKSSDSTASSSITHKFTIKPKDYMIDFNVELVGADKLLTQGSMNLSWQ
                     YAAAQQESDIDFEKQNTQVGFVKDGSFDYYTVGNRSSKDFDESVKWVGVRQRFFFTSL
                     IAKDNFTNGKMEWTVPPDSAKILVRSTANMKVQLPVASTSSTGFSIYYGPSDYHILKK
                     YDLNLEKLVNLGQGMYAFVRPLNRFVILPIFDFMRNLSGTSLGLAIALLTLFIRLLIS
                     PLTYSSYLSGAKMKALRPEIAKLKDKFGEDKQAISMEQMKLFREAGVNPLGGCIPALL
                     QIPIFFALFSFFNSEVSLRGAEFLWANDLSAYDAPIKFGFNIPLLGDHLSLFNVTAAA
                     TSFLISVYSMSMTPDQNNPVMKYLPYIFPVFLLFFFNKLPSALTWYYTVSNVVTLALQ
                     FVIQNYIIDHDKILAKIQENRKKPKTKSKWQERMEQMQDQQKKMKEMQQKNKR"
     gene            complement(28941..30590)
                     /locus_tag="PLO70_04590"
     CDS             complement(28941..30590)
                     /locus_tag="PLO70_04590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQZ73765.1"
                     /translation="MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGLRVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIFTSQANNVTTGRIYQTVINKE
                     REGAYLGKTVQVVPHITDEIKRRMQLLGQTGEYDIVITEIGGTVGDIESLPFVEAVRQ
                     LQWELPEEDSVVVHLTLIPYLKAAKELKTKPTQHSVRMLSQEGVHPDVIVCRTERTLS
                     TEIRRKIALFCNVKQEAVIESIDAATIYEVPVLMMREGLDKIVLRKMNITEYKEPELT
                     KWKEFLDKLKYPKGKVTIGLIGKYIELQDAYKSILESFIHAGAINECKVDIVNVHSEF
                     ITDDNVGEKLAGLDGLLVAPGFGHRGVEGKIVAVKYARENNMPFFGICLGMQMAAIEF
                     ARNVLGLKDAHSTEMNPDTKEPVIDLMEEQKKVTSKGGTMRLGSYPCEIREGSLASKI
                     YGASSISERHRHRWEFNNKYLELFEKAGMIASGKNPGTGLVEIIELPNHPFFVGVQYH
                     PELKSTVENPQPIFVHFVKAAKTFAEKKSEVKNPLLQSEMI"
     gene            30854..32134
                     /locus_tag="PLO70_04595"
     CDS             30854..32134
                     /locus_tag="PLO70_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ73766.1"
                     /translation="MKKSSLLVFVLLITLNLFSQKTYIWCGTLVDGVSEEPKKNMTIV
                     VEKNKIITVQNGFTTAGTSDKTIDLKTKTVTPGWIDMHVHMEFETNPNRYVEVFTSNP
                     ADYAFQSVKYAEVTLMAGFTTVRDLGGSGVNISLRNAINKGIIKGPRVYTAGKSIATT
                     GGHADPTNGYRKELMGDPGPAEGVANGAEECRQAVRQRYKDGSDLIKITATGGVLSVA
                     KSGKNPQFFEDEIDAIVKTAKDYGFKVAVHAHGEEGMKRAIRAGVNSIEHGTFMDDEA
                     IELFKKHGTWYVPTITAGKAVGDSAKIPGYYPPVVVPKALETGPQIQKTFAKAYKAGV
                     KIAFGTDAGVYKHGMNWLEFGYMIEAGMTPMDAIRSATISAADLLGEKDKLGSIEAGK
                     LADIVAVDGDPLKDAKSFGKVVFVMKDGMVYKQQ"
     gene            complement(32137..32934)
                     /gene="proC"
                     /locus_tag="PLO70_04600"
     CDS             complement(32137..32934)
                     /gene="proC"
                     /locus_tag="PLO70_04600"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQZ73767.1"
                     /translation="MAKKIAIIGGGNLGASIADGLILSEFSRPVELSITRRNLSTLSR
                     FAERGCMVHSDNKKAVRDSEVIILAVKPYNYADIVKEIKPLLNPKKHIVISVITGVWI
                     EQLHKAIGKPVPVIRAMPNTAIAIQQSMTCLSHAGASASQLNYTQQLFDQLGKTVMID
                     EKLMDAATVLGACGTAYAMRYIRANIQGGIEIGFDAATASIIAAQTVKGAAQLLLEKG
                     SHPEHEIDKVTTPKGCTIAGLNEMEHQGFSSSLIRGLVASYNKILKD"
     gene            33103..33663
                     /locus_tag="PLO70_04605"
     CDS             33103..33663
                     /locus_tag="PLO70_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ73768.1"
                     /translation="MKKKFFSLFTAVVLSSSIFIACNNEAETKTAEDSTTVSKEEPMA
                     PPAEAPKDIVDVAAASADHSTLVAAVKAAGLVETLKGAGPFTVFAPTNAAFNALPAGT
                     VDGLLKPENKVDLTSILTYHVVAGAVKAADLKDGQKVKTVQGAELTVSIKDGKVMIDG
                     ANVTAADLAASNGVIHVIDAVVTPKK"
     gene            complement(33722..34717)
                     /gene="rfaD"
                     /locus_tag="PLO70_04610"
     CDS             complement(33722..34717)
                     /gene="rfaD"
                     /locus_tag="PLO70_04610"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQZ73769.1"
                     /translation="MEKNKVILLTGAAGFIGSYLLGYLNKQGYKNIIIVDDFSEEDKW
                     FNFDSKDFIAKVEREELFDWLKREEPEIDFVFHLGARTDTTEFDYTIHEKLNVEYSKN
                     IWNYCTERNIPLVYASSAATYGGGELGYKDDHGLVERLHPLNPYGVSKNEFDKWVLQQ
                     TEGHTASHPPFWAGLKFFNVYGPNEYHKGRMASVIFHSFNQIKSTGKVKLFRSHRPDF
                     KDGQQLRDFIYVEDVAKVCYWLMEHTIISGLYNLGTGKARTFEDLVKATFAGIGKEPA
                     IEYIDIPEDIRDKYQYFTEADMNKLRNAGYKDSFYTLEEGVGDYVKKFLLLRNYY"
     gene            complement(34717..>35480)
                     /locus_tag="PLO70_04615"
     CDS             complement(34717..>35480)
                     /locus_tag="PLO70_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQZ73770.1"
                     /translation="GVTNCLNFGNPYDPEVYYQFVHAIKGMGEACRKFDTPVTGGNVS
                     FYNQNPDGPVYPTPTIGMVGLLEDVNKKMTLDFKEVGDAILLIGSSSDDINSSQYLSK
                     ICGVEFSPAPHFDLEEEFTMQQKITELISKGLIESAHDVSEGGLFVTLLESSFNNSLG
                     FDVVANDYNIRKDAQWFGEKQSRVVVSVKKEKLDVVKKVMGNHSFEELGLVTDGSVEV
                     DGMEWGHIEDWKEKYDSSIENYLSKEEAGSALSSI"
BASE COUNT        10852 a         7408 c         6659 g        10561 t
ORIGIN      
        1 cacgttgaaa tttggaagtg gcctggtgaa tgggtttact acagatatcc gcttatcaag
       61 tattagcagc gatggattta tagatcgtgc aaaaagcagc ttaaaatctt ttcatttttc
      121 tacagcttat ataggaaaaa caacttcact acgtttaaat gtattctccg gaaaagaaaa
      181 aacttaccag gcctggtacg gtattgctga agctgatctg aaagctggca acagaaccat
      241 caactatgca ggtacagaaa agccgggaga accctatgat aatgaaagcg ataattatac
      301 ccaaacccac taccagtttt tccttaataa gtccatcggt aaaaaattaa atcttaatac
      361 ggccttattt cttactaaag ggaaaggcta ttatgagcaa tataaagcag aggaggatta
      421 ttctgcatac agaatgcctt atcctgttaa tggaccagat actatatttt cttcggattt
      481 catccggcag ctttggctgg ataattattt ttacggtact atttattctc ttcactataa
      541 ggctgcaaaa accgaattaa caataggtgg cggtcttaca acatatgatg gaaatcattt
      601 tgggaaatta acctgggcct caaatgggct tacctctctg aaaaattggt acgataacga
      661 tgcactgaaa actgatttta atatttatct gaaacaacaa acacaattga ataattactg
      721 gaatttattc tatgatatac aattcaggta tgttaactat aaaatagaag gattcagaaa
      781 caaccccggc ctgattgtaa ataacaaatt cagttttttc aacccgaaag ccgggattgt
      841 atacaaaaaa gacagttgga aaagttattt atcctatagt gtagcaaaca aagaacccaa
      901 ccgtgatgac tttgaggcag gtaataccca acaaccaaaa ccagagagat tacaggattt
      961 tgaactgggt attgaaaaat cgactaaaca gcttaactgg tcagccacct tatattatat
     1021 gaaatataaa gaccagttag tgttgaccgg aaaaataaat gatgtagggg catatacccg
     1081 aactaatatt cctgagagtt ttcgggccgg gattgaactg cagggtggtg taaaattcaa
     1141 ttcatggata aacacaacag ccaatcttac tttaagcaga aataaggtta ttgattttga
     1201 ggaatacatt gatgattatg ataatggagg ccagaaaata aacagcttta gttcggcaga
     1261 cattgccttt tcacccagca tcaccggtgc cggcagtata aatgtattgc cattcaaaaa
     1321 cacagaaata agttttataa gtaaatatgt aagcaaacaa tttctagata ataccgggaa
     1381 caacagcaga agcctcaatt cattctatgt acaggacata agggcaattt acacgataaa
     1441 aaagaagtgg ctcaaggatg taactattac agggcaggta aataatttat ttaataaaga
     1501 gtatgaaccc aatggctata cctacagcta tatctggggt ggagaaacaa ttacagagaa
     1561 ttactatttt ccgatgtcag ggaccaactt tataattgca gtaaatatca ggttataata
     1621 caaggtagct taagccgaaa attgaaaact gtattttcaa ttttcggctt tattttccgc
     1681 tgccaaattt ttcctgccag gccaatacac cacctatcag gttctttgta ttcttaaacc
     1741 ccagtgtatc cagaaacata caagccatca tactgcgttg cccgctgcgg cagtgaataa
     1801 ttatttcttc ttctttcaag tcctcaatct cttctatctg catcctttga atgtctccca
     1861 acggtatcaa tttggcacca atattaaatt ctgcatactc aaatggctca cggcaatcaa
     1921 taagatttag tttttcgccg gcatctatac gggcttttaa ttcctcaacg gtaataatat
     1981 ccattcgcaa taaaaattaa atttcagttg tattttttga gctgtcagca acaggcttgt
     2041 ctgcctgaag ccatacatct atcaattgcc ctgaacgaat acgctgtatt cttttttcgt
     2101 catccaatct ttccgggctt tgcttatata tccatgcact tatggtatca gaaacattcg
     2161 catccaatac aatggcacca atgccaacac ctgccctttc caataaactt tttgcatctc
     2221 cgtatgtcat cccggtaata acaggtacca caaattccat ctttccgata ccatcaccca
     2281 ttaccaacga aatagcactt ccttttctga tctttgttcc cggtgttatc attgcacctt
     2341 cataccattg ttcaagaatc gcattctttg caaagtcagg cttgaaagtt gtatcaccaa
     2401 cccgaaggtc catactacgc aaaaccattt ctgcattcct gtaactatat cctatcaaat
     2461 taggcatttc cactaatggc ggcacagctc tgcggattat aagataaacg gttcggtttg
     2521 acttaaccac ttcatctgca tccgggatct gcctgattac tttcagcggc ggaatagaat
     2581 ctacataaac tgaatcttgt acttccacat caaaacctgc cttccttaaa atagaccttg
     2641 cctcttcata actcttacct gctacagaag gcaccgtggc agcttctccg tggccggtaa
     2701 gccagttcaa cgaaagtaga aataatgcaa aaattccgat cgaaagcaaa ataccaataa
     2761 tgacattaag ccataaggga cgatgtgtga taaatttaaa cattagcctg caaataaatt
     2821 atgaatgtag gtctcaggaa taaaagcact cctgaaccaa ttttgtataa aactctttta
     2881 aactccagcc cattgatctt acttgttgcg gtataatgct ggcctcactt tgtccgggta
     2941 ttgtattcac ttctaacata taagggcttc cttctttttc attatagata aaatcaattc
     3001 ggaccacccc cctgcaatta aaaacagagt atactttcct ggcagtatcc cgtattttat
     3061 cagcaatcgc ttcgtttact tcagccggag ttgtttcagt tgactttccc tggtatttag
     3121 cctcaaaatc aaagaatgcc atatctgcat gtgcttttac ttcagtaagt ggcaacacaa
     3181 taatatttcc cccagatttg aaaacaccaa ctgtgaattc ccgtccttct atcatttctt
     3241 ctaccaacac ctggttatcc tctttgaatg ctttttcaat agcatgatta agttcatctg
     3301 aaggagtatc aactttggac atcccgatac tggatcctcc attattaggc tttacaaata
     3361 ctgggaatct cagattcgca actacatcat caggtcttcc aaaattgttt ttaatcaaaa
     3421 aaacagattt tgctacgggc acccctgctg ccccagcaac agcagttgca taccttttat
     3481 tgaaagtaat agcggaggtc gcagcatcac aacccgtata aggaattttt aatgtatcaa
     3541 aatatccttg cagtttgccg tcttctcccg gtgtgccatg catacctatg aaaacggcat
     3601 caaaagtcac tttttcatta tccaattgaa gggagaaatc attcttattc acttcaagtt
     3661 tcctgccatc cgttaactcg tagaaccagc cctgggaatt gatgtcaatt ttgtaaacat
     3721 taaaaagatt tctgtccagg ttattatcga tagtaactgc gcttttataa gagatgactg
     3781 cttctcctga aaatccgccg gtcacaaggg ctatattctt cttcatcttt ttaatgtaat
     3841 gggttttatt cccttttaaa cgggacgtat tatgcaaata ttgcagaaaa aattaagatg
     3901 cccaacctaa aaagtagtta tcagatagca ttaacaaaaa atatcataaa gccttcatta
     3961 ttaaagacag atttagggga ttaccactcc tgccatgctc cttttttggg ggtagaaaat
     4021 aagaaaggtg aaggccgttt ctccttcacc tttttacgac gggacatatc acccgccact
     4081 cattaagcag ctttcgctgc tgtgtatatt gagatatgtt tgtaagttat gaaaaaataa
     4141 ctccaatgcc gctgtacgga tagaagaaat tatgcacaag ctgagcacaa aactaaatat
     4201 gcagcttatc ttttttagcc attaactgtt caacagtttc ctggtacata tcatccggaa
     4261 cacagcaatc caccgggcaa actgcagcac actggggctc ctcatgaaag ccctggcact
     4321 ccgtacattt attaggggta atataatagg tatccacgga tactggcgca tttttttgtt
     4381 ctgcatccac tactgttccg tcaagtaatg tataagaacc ttttacagtt gtgccatccg
     4441 ctactgccca ttccacgcca ccctcataga tagcattatt tggacattct ggttcgcagg
     4501 caccgcaatt gatacattct tcagtaatct taatagccat agtaattaaa ttattaggtt
     4561 cggggttact tttgctttac aaaattagtt gacaagaatg aatttacaac atcgaattta
     4621 tttattatca aggctgggcg aatatatttt atcaaccgaa gttgaatggc aaaaagccaa
     4681 agaaaaagct aactgggaaa atggatggtt tatccctgac tttgttgaac tggcttcaaa
     4741 aaatattgcc ctgtcctttt tacagaagga tatactggaa aaatgggcgg gcggccatca
     4801 aatcccggca tcttcaacca atagcagaac catcggtatt gtaatggccg gaaatatccc
     4861 tctggtgggc tttcatgact ggttatctgt tttcatcagc ggccataagg ccatcatcaa
     4921 actttcatca aaagaccagg tacttttcaa acatctcatt gaaaaattgg ctgaatggga
     4981 cccattggtc aacaaattca cttcattttc ggaaatgcta aaaggctgcg atgcgtatat
     5041 cgcaaccggt agcaacaatt cctccaggta ttttgaatat tatttcagaa aataccccac
     5101 catcatccgc aaaaaccgga cttcaattgg aattttatcc ggaaaagaaa cgaatgaaga
     5161 attgagaaaa ctggccgatg atgtttacct gtatttcggt ttaggttgcc gcaatgtgac
     5221 aaaaatttat gttccatcgg gctatgattt tatcccgtta ttggatgctt ttaacaaata
     5281 caggcacctt gctgatcata acaaatacaa aaacaattat gattacaacc ttgccctgca
     5341 cctgctgaat aaaaaatatt acatgaccaa cggctcgatc ttactggtgg aagaaaccgc
     5401 atttttttca cctatcagcc aacttaatta tgagtattat tcagatacag acaaattgtc
     5461 ggatagtctg aaaaatcatc ctgaattgca atcgattaca ggtgaagggt atgtcccttt
     5521 tggccaaagc cagtacccgg ctatttcaga ttatgctgat ggtgtgaata cactaaaatt
     5581 tctaagtaca ctttgaactt aaattcaggt aaattgtaga aattaataaa tttaccgggg
     5641 tcctgaagta cgaagccttt agtaaagatt tgttaaacta aactattggt atgccaagat
     5701 gggctgacag tttgttattt tgtataacag aggcatatga tttgtaattg aaattcgaaa
     5761 aacaacctat taaaagaaaa gaatcactat gaaacttaaa cagctatttc tgattatcgc
     5821 cgtaagtgca gtttctgctg taagcagtgt ttggatttac agcaaagtgg ctgcaaagca
     5881 gcaatcttcg attttctcac aaagcaatga aggcaaggtg ccggtaaatt atgctggctt
     5941 tttcgacaat gcagcttcga cggttggaga agttactgat tttaccaaag cggctaatgc
     6001 agctgtacca gcagttgttc atattaaaac taaaataccg gcaaaaaaag taactaacca
     6061 gcttccccgg aaccgtggta acagcatgga tgattttttt gagcagttct tcaatttcgg
     6121 accacagatt caacctgagc aaagagcttc aggaagcggt gttataataa gtgacgatgg
     6181 ctatattata acgaataacc acgtcatatc agacggaggc gaaggcgttg ctgatgaaat
     6241 aacagtaacc ctcagcaata aaaaaaccta taaggccaaa gtgattggta aagaccccag
     6301 tagcgatatt gcagttttaa aaattgatgg aactggtttc ccctttctgc tttatggcaa
     6361 ttctgataat gtgaaactgg gccaatgggt attagcaatc gggtacccgt tgacattgga
     6421 aacaactgtt acagccggta ttgtaagtgc caagggaaga tctataggaa ttaacggacg
     6481 tcaaagtcaa actccaattg agtcattcat ccagacagac gctgccgtga accagggtaa
     6541 tagtggcggt gccttgatca ctactgacgg acaactgatt ggcatcaact ctgcgattta
     6601 tgcacctaca ggaacttatg cagggtattc atttgccatc ccggtaaact tggctaagaa
     6661 gatcgtaaac gatctgataa agtttggaga tgtaaaaagg ccatatttgg gtattgctgc
     6721 agaccaaaca aaagttaact caactgatgg tgcctatatc ggtacggtgg ctaaagatgg
     6781 cgctgcagct gctgcaggtt tgaaaaaagg tgatatcata aaaaaaataa atgatgtaac
     6841 aatcagctcc tggagcgaat tacaaggcac tgtagcatcc ttaaataccg gtgaaaaagt
     6901 gaatattact tataagagag atggtaagga atatacaacg agtgctaccc tgaaagcaga
     6961 aactggaaca tatgacaacc ttgccgcatc aaaccttggt gatgtactgg gtgctgaatt
     7021 agaaaacctg gataaaaaga ccgctgaaaa gaatgaagtg gaaggtggtg ttgtagtgaa
     7081 aaaaattaaa ccaggtggtc cattcagtaa aacaagaatg caggacgggt tcattattac
     7141 cagcgtaaat ggtgttgata ttacaaatac tgaagaatta agccgtgcta ttgcaagctt
     7201 gagaagcgga agcttgaatt tggaaggctt ttatgatggt tatgatggca tgtacaggta
     7261 tcctgtaaac cttcagttgg aagacgaata aaaaaataag tataaaaaaa taaaagccgt
     7321 ctcattcaac gagacggctt ttattttaaa atctattcgg ctcagtttat tatttttcgt
     7381 acaccaaata ctcccagggc tgcatctcaa aacctttctc aacagtaaaa tcattagcag
     7441 cacctgaaaa cacattttta aaagtacccg tcaatctttc gtctataacg tcaaaatgca
     7501 ggttgttttg tgatgaaagg tttaacaaaa ccagtacttc tctgtcacca ctttttctta
     7561 tataagcaac tattttctgt ggatcagttg ttttaatgtg aaaagtcttt actgttgtat
     7621 caccaccacg caacgccgga ttatttgtct taaggtgaag cagtgtctta taaaaatcat
     7681 gcaataaatt attaccggtc catgaaatag tatctttatc aaagaactca atccgcttct
     7741 ggttaggcag ctcctgtcct gaatacaaca gcggcactcc attccatgta gcgctgaaca
     7801 cggctaaagc ttttgccata tcaccgtatt tttcatattc tgttccattc cagctgttct
     7861 catcatggtt ggtagtaaac caggcccgca tggtactgtc gccgaggtca tcatacttct
     7921 taagtataac atcaagcgta tctattgaca attgctgctt ataaaactct tctgtcttgt
     7981 gcatccatgt ccatgtgtaa ctggcatcga atgcttcccc gtattccggc ttttccagtt
     8041 catcaaattc tcctaaccaa aacaaaggtt ttacagcatc aagctggggc cgtgcctcct
     8101 tccagaaatc aagttccacc caaaaggcta ggtcacaacg gaaaccatca atatcacatt
     8161 ccgtaaccca aaatttcatg gcatctatca tagcttttcg caaatcagga ttcttgaagt
     8221 ccagctcaat aatatcttcc atgccggaag ctatcttaaa atcattagtt gcagaatctt
     8281 tcaaataata atcaggatgc tctattgtcc atttatgatc ccagccagta tgattggcta
     8341 cccaatctat gattacctta aaacccaatt catgcgcctg tttcaccaga ttcttaaaat
     8401 catccagcgt tccaaactca gaattaatgg aagtataatc cgaacaggca tagtaacttc
     8461 ccaatgatcc ttttttattt ttttgtgcga ttggggtaat aggcataaac caaagtgttt
     8521 ctacacccat atcttttagc ctgggcattt cccgggcaaa ggcattaaaa gttccttcaa
     8581 tggtatactg ccttatattg acttcataaa tattggtgct gtgcacccaa tccaatgtct
     8641 tgaacctgcc ggtcatatta ttactttttt ttgtttcaga atcctctttc ttcttcatca
     8701 gcttgcagga acccagtata gctgttaaag aaagggcaag ccaaaaaaat cgcatatcac
     8761 aagttttgcg ggaagttaag aagaacattt gatgtgattg attgttgtta caaacaacta
     8821 attcagtatt gttaactttg agccatgaag agttttgaag tccccattat ctatcgtagt
     8881 ccattgattt ctgcaattaa gaagaagcgg aaagaaacag ataaaatgaa aaaggatttt
     8941 tcccctagcc tgctggattt cgggccaata catatttatc tggcccgtca ttttggtttt
     9001 tgttatggtg tagaaaatgc cattgaaatt gctttcagaa ctgtagaaga aaatcccggc
     9061 aaaaagattt ttttattaag tgagatgata cataacccac aggtaaatac agacctcctg
     9121 gcacatggta ttcaattttt gcaggacact tatggtaaac aattgatacc atttgaagaa
     9181 atatctgctg acgatattgt gctgattcct gctttcggaa caacattagc gatagaaaaa
     9241 aaattacagc atatcggcat ccaaaccgaa aaatataata ccacctgccc ttttgtagaa
     9301 aaagtctgga accgtggtga agccattgct aaaaataagt acacaatcgt aattcatggc
     9361 aaaccaaaac atgaggaaac cagggccact ttttcccatg cttctgctaa tgctccgtct
     9421 attgttataa aaaatatgaa tgaagccgta gagttaggaa aatatatcac aggtgaaaaa
     9481 tcagctgaaa cgttctacac tgaattcgaa gggcaatatt cgcagggatt tgatgcaaca
     9541 aaagaccttc aaagaatcgg tgtaataaac caaaccacca tgctggccag tgacacacag
     9601 gccattgctg attatctgaa aaaaatcatt attggaacct tcaattccga tgatagtttt
     9661 gctgacaccc gggatactct ttgctatgcc acaaatgata atcagactgc cgtcagcggg
     9721 atgttggaga ctaaagccga tctggcaatc gtagtgggag gctacaactc atccaatacc
     9781 tctcacctgg tagagttgtg tgaagaaaag ctgcccacct attttattac taatgaggaa
     9841 aagattctat cccccagaga aatccttcat tataactttc atactaagaa tgaattactg
     9901 acaactgaat acctgccttc taaagagccg gttaaagtct taatcacaag tggtgcttcg
     9961 tgtccggatg ctttagtgga aggagtgatc cataaactga ttcaattttt ccctgtttct
    10021 aaatcgatgg atcaattgag agaagagttt caataaaaac cccctgacaa aatcagaggg
    10081 ttgctatctt tcgttatcct gttacttacc tatacaacaa gtcataatat tcatgtgcca
    10141 tacggttgct gtcaaactgg aagcgaacat cctgcattcc gtttttcatt acctgtcgcc
    10201 aggtactgta attctcgtag tacaatggca gcatttcatt ctccagtatt tcgtatagtt
    10261 tattcaggtc atactgatcc tgttcatgta cagccatact ttcataatct gccatgggta
    10321 ctacaaaacc attatgacca tgattaataa actctggtat ccagccatca tctgtggaaa
    10381 aattaacagc cccattcata gctgcagtca taccactggt tccggatgct tccctgggca
    10441 ccctgggatt attgagccag caatctgctg cctgcttcat tcttttggac aaacctaact
    10501 catacccaat taaaacagcg acatttttat accctttgct gagatgaacc aattggttga
    10561 actcactgat tgcaggatga tcaaccgggt aaggttttcc agcccagatg atctgtacgg
    10621 gatatttttt gctatttatg agcttttcaa atcgctcttc agcagttgta ataagccctg
    10681 ctcttttata accggcaaat ctccttgccc aaacaatagt gaaaacttca ggatcgaata
    10741 tcttaccggt ttgatctgcg acaatttcaa aagccctttt cttcaaatat tttttcctgt
    10801 catcaattcc ataatcatcg ccggcctcca tgaaacgata caattgctta tccgcccaat
    10861 acctccagtt ttgtgcattt gttatagaaa ttatcgggca tatattttca tatttctccc
    10921 acatcgctct tgaaacacgg ccatgcagct gagaaacacc attggctaac ctggcaaaac
    10981 gtaacgctgc cagggaatga ttgaactggt cgctgtcatt gtcataaagc tttttaactt
    11041 cctcaacgga caggccgcag aaataactca tcttatggca taaataaata tcatgttttt
    11101 cattgccagc ttcctcagga gtatgcgtag taaaaacaag acgtttttta acttcctgaa
    11161 tattattact gaacttttta tagaggtaga atgcagctga taatccatgg gcctcgttca
    11221 ggtgataaag ttccggattg aaacccagta aatcaaccag cttggcccct cctactccca
    11281 gtaaaatgaa ttgagctact ttagtcgcca catttgcatc atacaaacga tgtgtgattg
    11341 tttgcgatac gtaatcattc tctggcaaat cagtagataa taaaaacagc ggagcagttt
    11401 taaaagtttc agggttcagg taaagggctt ttacccacac agaatgctca tgtattgtta
    11461 tctggaactt aataccagtg tcttcaagaa agctatagtg cttttcattc caggctacgt
    11521 ccagggtctg gtcctggttc ctttcctggt cataatagcc atatttccat aaaatgccta
    11581 caccaattaa gttttgtcgc aattcatagg cacttctaag atgtgatccg gccagaaacc
    11641 caagaccacc actataaatc tttaaaggct gatgtgtggc aaactccata gaaaaatagg
    11701 ccactcgctt actataacgt tcatctacag gatacggaac agaaaatttg gtaaaactca
    11761 tattgtgttc tttttacacg aagctgcaat ataaaactta atcctcaatt aaactaaagt
    11821 gctcattatc aggcaaattt atccacacaa aaatgttaaa aacacaataa acaaaattgt
    11881 aatgagggtt tgtaactcac ttcttatcag attttttaga ggccgcctgt ttattgtttg
    11941 attgaatcgg tttcacatat ttttttctgg caaaactccc atcaaatgaa caatttaatc
    12001 cccttcttga accgcaacct tcctctttca acgaaacaga attagatgtg aatttgaatt
    12061 gcattacaca ggggtcccca tcctgcctgt attcggccaa atttgaacta atccatctcg
    12121 cctctccttt taactcgccg gtacattcgc cattattttt ttcaatgtga ataaagaatg
    12181 ttatgcggtc actttttcta ccgtcccgta cagaaaccag gttcattttg ccggagctgt
    12241 aatcagctga gtatttattt ttcctgggta atgtatcaat aggattaatc agttctgtta
    12301 ctttatcctc taacgcttcg gtcataataa gcataaagtt tctggcatca gcattcaaca
    12361 catacacatc tttcccttca ctgatactgg catcggggtt ttttcgctga actgttttgg
    12421 taatagtgta ttttctgtca attgttacag actgtgtagt ggcttttttc tgatccggaa
    12481 caagaaccgg aatagaagcc agatactgtt ctttcttgtc aaatgccaaa ataaaaagga
    12541 cctttttggc aggcgtaaca gtctttacaa gcaggtaagt ttctgctttg ggtactgcag
    12601 ctttacctat tgcgtatatt ttcggtttaa tccccttacc aaacactttt cgcaaaactg
    12661 aatctggtac aaactctgta aatattttgt agctgatcag taaggaatcc ttatcctttt
    12721 tatccagtat cgtatctcct acctggtatg aaagggaaag gggctgaaaa aattctataa
    12781 aatcagatac ttccacggga tcttcaccgg ataatattgg tttctttttg ggtttgcagc
    12841 ctgccaagag taaagctcct aacaacaata acaggaattg ttttaataca gccatagcgt
    12901 tcattaacat gccgaaaata agtgtttccc ggggttctgt caacttgaaa tttggcaaaa
    12961 acaatcagat attttagatt tgtctaaatt tatttttagt catgttgaat tacacaacca
    13021 gtgaagagaa ttatataaaa accatccttc atttgcaaaa acaggatgga acagtaacca
    13081 ccaacgaact ggccaacgaa ttaaagacta agccagcttc ggttacagat atgatgaaga
    13141 agctgaaagc taaaaaactt gtcaactatc aaccctacca gggttttcgg ctgaacagtg
    13201 aaggcaccag ggtagcgcta ggtatcatcc gcaggcacag attgtgggaa tattttttag
    13261 cggaaaaact aaaattcagc tgggatgaag tacatgaggt ggcggaagac ctggaacatg
    13321 taagcaacaa aaaattaatt gataagcttg atgagtttct tggtttccca aggactgacc
    13381 cacatggaga ccccatacct gatgccagtg gcaagataga gatcagtaaa aaaatatgcc
    13441 ttacagaatt aaagcttagt tcaccggcag ttgtaatcag tgtcagcgat caatccagtg
    13501 aaatcctgga attacttgaa cataaaaaaa tcgcaatagg tacaagactg gaaataaaaa
    13561 agagatttga atttgacaac tcaatggaaa tacgaattgg ccgccaaccc gtatttacca
    13621 tcagcgaaca gttggcaaaa aatattttcg tacagagtaa ttcataaaat atggaagcta
    13681 ttgaaaaatt ttttattgaa atagggccgg tttggtcagc attactggct acaacattta
    13741 catggtttgt tacagcactc ggagcctctc ttgttttttt ctttaaaaca cttagccgtg
    13801 gagtactgga ccccatgctg gggttcactg gtggtgttat ggttgcggca agtttttggt
    13861 cactattgaa tcccgccatt gagatttcag agaagctgta tccatccatg agctggatgc
    13921 cggcagcagt tggcttttta ttgggagccc tttttatttt cttcctggat aaagttacac
    13981 cacaccttca cctgaatttt ggtgttgaag aaacagaggg ggtaagaacc aaattgcata
    14041 aaacaacatt attaattctt gctattacac tacataatat acctgaagga ttagcggtag
    14101 gtgtattatt tggtgctgca gcaataggta tggaagacgc ttcaatatct ggtgctatca
    14161 ctttggcaat tggtattggt attcagaatt ttccagaagg tattgctgtt tctatgccgc
    14221 tgagaagaca tggtgtaacc cgtttaaaaa gcttttggta cggccagctt tcagccattg
    14281 tagaacctat agccggtgtt gttggagcaa tggctgttat atatatgcaa ccagtattac
    14341 catttgcact ggctttcgca gcaggcgcta tgatatttgt ggtagtggaa gaagtaattc
    14401 ccgaaacaca aagggacaag tataccgacc gttctgtact tggcttcatc ggaggttttc
    14461 tcgtgatgat gatccttgat gtagcgcttg gataaaaact aatttgctga aactttatgc
    14521 gtacggataa ggtgtttgaa cctgctgaat gtttcaggtt taatattcag atacgatgct
    14581 aaaaattttt gtggaacccg ttgcataagc tccgggttct ttgttacaaa attcaggaat
    14641 ctttcccttg gtgtcatttt caccagttgt atctgccagc ggtctttgat caccatggaa
    14701 taagtaacga tcaaacggcc caggtgttcc attcgctgtg aagaagcgaa cattttttct
    14761 gcatcatcgt aactgataga aacgacagta gtgggttcaa ttgtttcaat aaaataatct
    14821 gatggttgac ggctgtgaaa ggactcctgg ctcagcaaca tctgcccctc aaatgagata
    14881 tgggtattaa tttcatgatg attcttttta taatacttac gggccaggcc cttcacaatg
    14941 aaattaaaat agttctcgat ctcaccagcc ttagtaatta tttccttttt cccaaaccgc
    15001 ctcactttac aaataggtaa aagattcctg ttaaactcat cttctgtcag gtcagtgaat
    15061 ctttgtaaat agttataaaa tgccttgatg gaatccatac tatgattata caccatataa
    15121 gttatagttt tttattgatt taaatcaata atgaaaaaaa gattcaaaga tgaaattagc
    15181 cctagcggaa atcctggcct atgacattag tcacattcga taataatgta agtttgtagc
    15241 cctgataagt gacaggttct caatgtaaac ccaaaataac ccccttatga cacaaaagat
    15301 caaagttgct aacccggtag tagaactgga tggtgacgaa atgacccgga taatctggaa
    15361 attcatcaaa gacaaactga tactccccta cctggaggtg gatattaaat attatgacct
    15421 tggtattgaa taccgtgacc agaccgatga ccaggtaaca atggatgccg ccaatgccat
    15481 taaagaatac ggcgtaggta ttaaatgtgc cacaatcaca cctgatgaag caagggtaaa
    15541 agaattcaac ctgaaacaga tgtggaagag ccccaatggt actatccgga atatcctgga
    15601 cggaactgta ttccgtgaac cgatcgttat cagcaatgtc ccaagactgg ttaccaactg
    15661 gaccgctccg attattgttg gccggcatgc cttcggtgat caatacagag ccaccgatac
    15721 ggtaataaag ggaaaaggaa agctcactat gacctttacc ccggaaggtg gtggcgagcc
    15781 tcaaaccttt gaagtattca actttaaagg tgatggtgtg gcgatgagca tgtacaatac
    15841 tgatgaaagc atcaagggtt ttgcaagaag ctgcttcaat atggcgctga gcaaaaagtg
    15901 gcccttatat ctgtctacta aaaacaccat cctgaaaaaa tatgatggcc gcttcaaaga
    15961 catttttgaa gagatctttc aaaatgaatt taaagcttct tttgatgctg ccggaattgt
    16021 atatgaacac cgcctaatag atgatatggt ggccagcgcc ttaaaatgga atggcaattt
    16081 cgtttgggcc tgtaaaaatt atgatggtga tgtgcagagt gataccgtgg cacagggttt
    16141 tggttcgctt ggtttgatga catctgtact gataacccct gatggaaaga ccatggaagc
    16201 tgaagcggcg catggtaccg taacaagaca ctaccgtgac caccaggctg gtaaacccac
    16261 cagcaccaat cctatcgcaa gtatttttgc atggacaaga ggtttagctt ttcgtggcaa
    16321 attagatagc aatcagccgc ttatcgattt ctgtaatacc cttgaaaaag tttgtattga
    16381 aactgtagaa gaaggtaaaa tgaccaaaga ccttgccgta tgtatacatg gcaataaggt
    16441 aaaacatggt gaccattttc tctacaccga agaatttctt actgccatag atgagaacct
    16501 gaaaaagaaa ttgaaataag gagtttctta agactacaac ccggccaata agccgggtta
    16561 tttttttaca cggttaatcg tatttgaaac tttgcagaac aacagtacag ctctataatc
    16621 ttcgccaaat acataaattc gtttgcctag aaacattcaa taaatttttc atgatgaaaa
    16681 aaagacaaga ctatatttcc tgggacgaat attttatggg ggtggcttta ttatcagcca
    16741 aaagaagcaa agatcccagc acacaggttg gagcctgtat tgttaatcaa aaaaataaga
    16801 tcgtcggtgc cggttataac ggactgccca ttggctgcag cgatgatgaa ttttcatggg
    16861 ataaaaaagg tgaattcctc gataccaaat atccctatat ctgccatgct gagctaaatg
    16921 ctatcttaaa caatatagga atggacctgc agggttgcaa aatatatacg gccctgttcc
    16981 cttgcaacga atgctccaaa gcaattattc aatccgggat aacagaagtc atttacctgt
    17041 ctgacaagta tgagcaggat gatattttca aagcttccag gatcatgctg gataaggcag
    17101 gaattacctg caggaaagtt gaaaccagga tcatgagcct tgaactatca ttcagagaaa
    17161 ctgatgtgta aggtctccgc ggtctaagat tcctcccaaa ataaaaaacc atccgccgta
    17221 atgacgaatg gttcagtgca attgtgttgt atcttttaaa ccctgaagtg agcaaaagcc
    17281 ttgttggctt cagccatacg atgggtatct tctttctttt tgaatgctgc gccttcacct
    17341 ttactggcag caacgatctc gccggctaat ttatcggcca tgctgcggcc attcctgtcg
    17401 cgactataac ggatcagcca tttgatactt aaagaaatct ttctgtcagg acgaacctca
    17461 gaagggatct ggaaagtggc cccacctata cggcggctgc gaacttccac tgcaggcgat
    17521 acattagcca aagctttctt ccaaacttca tatccatctt cgttggtttg ttttgctact
    17581 ttgtccagcg catcataaaa aataataaag gctccgcttt tctttccttc ccacatcagg
    17641 ttattcacga aacgggtaac aagtttatca ttaaacctgg ggtctggtgc taaagggagt
    17701 ttctttgctt gtgctttccg catgacttat tcttgaatta cttattaaaa ttatttttta
    17761 gccttttctt tcttggtacc gtatttagaa cggctttgct tacggtcttt tacaccggca
    17821 gtatccagtg aaccacgaac aatatgataa cgaacacccg gtaaatcttt tacccttccg
    17881 ccacggataa gaacgattga gtgctcctgc aggttatgac cttctccggg aatgtatgca
    17941 ataacctcga ttttgttggt taaacgaacc ttcgctactt tacgcaaagc ggagtttggc
    18001 tttttaggtg tagttgtata tacccttgta catacaccac ggcgctgcgg acactgatcc
    18061 agtgcccttg atttgctctt agccctgata atttctcttc cttttcttac taattgttga
    18121 attgtaggca ttcttgaccc ttatttttgg gagggcaaag gtaaaggcag acgtcttaat
    18181 ctccaaaaaa atgacgactg atttttctgg ttttgaacct tttggggata aaatagcccc
    18241 aatttaagaa ttgaggctat ttccgtttca caatccatta taaatcagtg tttatacagg
    18301 ctgcatccag ccaagtttat cttcagctct tccttcgcgg atatcagtga gccatttttt
    18361 tattgccgga gccgttttcc agctatccac atcaaacttc atagaaaaat ctttgtaacc
    18421 cagttcttta atcggagata tggtggctgc tgtgccagta ccaaatactt cttttaattc
    18481 cccggcttta tatgccgcaa ttatttcatc aatacttaac tgcctttcct ccactttgag
    18541 ccccagttcc tgtaataagg ttataacact ttgtcttgtc actccaccca ggatagttcc
    18601 ctgttcaagt cctggtgtaa tagcggtgtt accaatgatg aaaaaaacat tcattgtacc
    18661 acactcctgt acatatttat gttcaaaagg atccatccac aatacctggt catacccctt
    18721 ctttttagct tcagcagtgg catacatagc agcaccataa ttacctgccg cttttgcaaa
    18781 acccacacca ccggtaactg cccttacata tttctcttcc acatagatac gcatcggcgc
    18841 actatagtat ggtccggatg ggctcataat tatcaaaaac ttgtaattat ctgaaggacg
    18901 taccccaatc aattcgtcag atgaaaacat gaacggacgg atatataatg aatgatcctc
    18961 tttagaaggt atccagtttt tatccagccc tactaactga agcatccctt ccataaataa
    19021 ttcttcgggt acagccggca tctgcattcg gtctgcagat atattaaacc gcttaaaatt
    19081 atcatgcgga cggaatatgt atgcattacc ttccttgtct ttgtaagctt taatgccctc
    19141 aaaaatagcc tgcccataat gtaaagcggc tgttgaagga cttagtaata agggctgata
    19201 gggcttaatt tccgcatttt tccactcacc attttcaaaa tctgcctcca gcatgtggtc
    19261 ggtaaaatat tttccaaaag gaagattatc aagattcaaa tcctgtaatt tgctcctttc
    19321 tgcttttgtg attgcaatat ccatagctgc taccataaaa ctttattttt gaaaggccaa
    19381 agaaacgaaa aaaaactaac acctattagt acaattatca ataatctcat ggatttaaat
    19441 catataaaat tacctgccac agttatagct gatttgtata aatcttccct tgttgaaagg
    19501 gaagaaaatc tcacaaaaaa ggaagctcct gcaaccctac caaaacagga aattcgacca
    19561 gctaatacag aaattgaagt aaaaagctgg aaatcattgg gcaataatct gaaacatatt
    19621 atggttattg taaacaaaac ggatgttatt tatcttccgg ataatgagct gatttttctt
    19681 accggtatcc tggccgcctg caagttgaat attagtgatg tagctatctt taacctgggc
    19741 aatcatccgg gcactaccta taaagatctc gtcagttatt tcaaaagtaa aaatgtattc
    19801 ctttttgggg tggagcctgt tgattttggc ctgccaatga atttccccca tttccaacaa
    19861 caaaaattta caggcattac atttcttttt tctccttctc tgaatgagct ggaaaatgac
    19921 aaactcctga aaagtaaact ttggatttgt ctcaaacaga ttttcaatat ctaaaactat
    19981 gttttgttat gaagttgatc tttgcaacca ataatcagca caaaatagat gagatccgtt
    20041 cagtactggg caaaaaaatt gaaataatta cgcttaaaga agcaggaata aacattgaca
    20101 ttccagaacc gtataaaaca ctggaagaaa atgcctccgg taaatcgaaa accatttttg
    20161 atataacagc taccaattgt ttcagcgaag acactggtct ggaagtgaat gagctgaatg
    20221 gagaacccgg agttaaaagt gcccggtatg ccggagaagg ccggtctttt gatgccaata
    20281 ttgaaaagct tttaagtaat ttgtcgggta aatcagatcg taccgccagg ttcagaaccg
    20341 ttatatcatt gttaattgat ggaactgaaa ctttatttga aggtatttgt gagggccgga
    20401 taattgagaa aaaaaggggg ctgcagggct ttggctatga tccggtattt attccgacag
    20461 gttccgataa aacttttgca gaaatggaca tagtagaaaa gaaccgattc agccatcgaa
    20521 agaaagctac cgaaaaattg gtagcatttt tgaatactat ataaacaaac cactgatttt
    20581 gacacctgaa cgcaaccaaa atatctccga aaacgacctt attaatggat gcatggaaga
    20641 caaccggcga atgcaggaag aaatgtatcg ccgtttctct ccccggatgt atgcggtttg
    20701 cctgcgttat gcgggcaacg ccgaagaagc cgaagatata ttacaggaag gatttattaa
    20761 ggtctttaaa aaactggaca gtttccggag ggagggttcc tttgaaggtt gggttcgccg
    20821 catttttgtg aacactgcaa tcgaacattt tcgccgcaag cgctacctga tgccggttac
    20881 agaaaaagaa gaaaatataa ttgaaggaaa atatacatct gtactggatg aactgggggc
    20941 aaaagacatc atggcattga tccagaaact atctcccggg tatcgtacag tttttaatat
    21001 gtatgtggtg gaaggataca cacacaaaga aatagccgaa atgcttggca tcagtgaagg
    21061 aaccagtaaa tcacaactgt caagggccaa agtgatattg caggatatgg ttagaacata
    21121 tattgataca caaagaggca taagtacaaa gtaatgaaca ggagtttgcg agataagatg
    21181 tataactatg aggcaacgcc tccagccgga gtatggaatg gaattgaggc tgagttgaac
    21241 gaggctatga ccaccaataa attatcggca aaaatgtacg gaatcgagtt agcccctcca
    21301 gcaatggcat ggaacaatat agctgctcaa ttggacgaaa caactttatc aaataaatta
    21361 tataatgcag aggcaatccc ccctagggca gtatgggata aaattgagtc tttacttata
    21421 cttaatgacg atacaagggt taatcaaaaa agaaaattat atcctgctct tcgttatgct
    21481 gcagcagcaa ttgtgatcgg ttttgtagtt tttggcgctg tgcggttagc acaacccaat
    21541 aaaagtgaag gggatgtagt aatgaaagaa actgtcattc ctgaaaatta taatacagaa
    21601 gcatcggcta caaaggatga gatacataaa ctaattaata atattattgc cgacaatgat
    21661 gaagccagga atgatgcggc actggaagcc agtaaaaaaa cttatgccag gcttgatata
    21721 ccttcccgga gcatagcaaa agaagtttcc aatttctatt ttgcttcgaa caacatttta
    21781 actcccggta caagagggtt agatatagaa gacactgctc cgcaggcaaa ctcaccaacc
    21841 gaccgctata taacattgat gacaccagaa gggaatatta tacgtatgtc aaaaaagctg
    21901 ggcgacttag tatgctgtat ttccggtgaa acagatgacc cgcaatgcaa agaccagctt
    21961 aaaaaatgga gagaaaaaat cgttgcttcc ccactaggtc actcttccga aagtttcatg
    22021 gacatcctaa atcttattaa ttccttacag gataataatt accaataatt cctgttttcc
    22081 taattttatt gcaaacaaat ctaatggcaa gaataaaaat tgatataccg gtatcttttt
    22141 ctttttcatg tgaaattccg gttcgaatta cagatatcaa ctacgggggc catgttggga
    22201 atgacactgt gttaacaatt attcacgaag caagaatgca gtttctcggt aattcaaatt
    22261 acactgaaat gaactttgcc ggtgctggat tgatcatggc ggatgcagga attgaattta
    22321 aaagtgagtt gttttatgga gatgtggtga gagtttttgt aacagccggt gaagtttcaa
    22381 agatcggatt cgatctctac tacaaattag aaaaactggt cgatgataaa aaggtgctgg
    22441 ttgctgtggc aaagacagga atgatttgct atgattacag caaaaagaag atcataccgg
    22501 tacctgataa agcaagggaa atcctgaaca ggtaattcag ctgttccata ttctccagct
    22561 ttcttcggcc tgtaatagca gcatctcctc accggtcttt actgccgcac cccttgcctc
    22621 tcccttacga agaaataatg tcattgcggg gttataaacc aaatcatata gcaaatgtct
    22681 gtctgatatg aattggtatg ggatttccgg gcaggtatct gtatccgggt acattccaag
    22741 aggtgttgta ttaattatga cagtattatc tataataatt tcagcattaa tctctttata
    22801 ggtaatagca gcaccttcat gtattttgcg gctaactatt gtgaagtcaa ttcccagcct
    22861 ttgtaataca aaaactactg cggctgcagc gcctccgtta cccaacacta acgctttctt
    22921 atgatatgat cgcaacaatg gtcgtaaact cttttcaaaa ccggtaatat ctgtattgta
    22981 tccaaccaat ttgccgttgg ttatattgat gcaattacag gcatccagcc ctctggggat
    23041 atttgtctcg tcaagaaaag aaaaaacctg ttttttgtat ggtatagtta cattcagacc
    23101 ttccagctca gggtattccc gtaacaaaaa aggtaattcg tctatggttt ttagcggaaa
    23161 cagatcatat tggcattcag taatttcttc ctgctcaaac ttttctgtaa aatatttctt
    23221 tgagaaagaa tgagacaatg gatacccgat taatccaaag cgcctcatca ggcagtctct
    23281 tttttatcaa ggtataaatt aaatgtatca ccgcgaagac caatccgcat tgcttcaaga
    23341 ggtatcactt ctgccggagc aatattgccg aggttggcat tgcaacccac taattcaata
    23401 aagtataatt gttgtgcctt ttgcggagcc tcccatataa ttttctcagc cgggatttgg
    23461 gttaatattt cctgtaccaa cccttccctc acttcaccac tgccacgata gatacctacg
    23521 ttacctgctt cacgggcctc agcaatcaca taggctgaac cagcttctaa ttcggctttc
    23581 ataagctcta tccatttgta tggcgggata atatgagcgg catctttact tcccacttca
    23641 cttaggaccg ttccatgctt tgttagtttt tctatatacc cacatttttc agcatgggga
    23701 atcgttatgg aaccatcact gacttccata tagctgatac cataatcctt gcaaacactt
    23761 atatagtcat caaactggtt acgtataaga aacgcctcga ataaggttcc tccgaaataa
    23821 acaggaacat taaatgaccg gtacacttcc agcttctccc ttaaattggg tgtaacaaaa
    23881 gaggtaccaa atcccaattt tacaatatca acatgcggat gtgaaacact cagaaaattt
    23941 ttagcttctt caatacttaa acccttatcc ataaccatgg tgagaccaga ctgacggggc
    24001 ttttgttttc tatctgggat ctgggacaaa ttgaaattca ttgttgtatt atttgaatgt
    24061 aataacgaat gcgatggccg caaaaatagg gaagaatgac cacagaaata ttgatttcag
    24121 gacagtacaa atttctggtg atcagataga cttatttctt ttaaacctgg ccagcatatc
    24181 cactacctgc tgattttgaa gaattgcagg atttaactca acaaattttt tcaaaagctt
    24241 tggagtattt accattgctg agtccaattg caataaggcc tcctttgtct tacccattgc
    24301 aaacaataca gctgctttat aaaaaataaa cagaggttta ccattggtaa catgcaaagc
    24361 cgcctctgcc tgcaataaag cctcctcaag aaaatctgct ttataaaggc aacgtatcaa
    24421 ggcttcccat cctccggcat tttttggtcg tatgcgtaca acagtggaga aatactgaat
    24481 agcatccctg aataacccca gttgcatctt gcattctcct gccagtagat tatattcagg
    24541 ttgcaagcga tggatcttca tagcggtatc aagctgctta atacatgcct gccattgtcc
    24601 ttcattgtag taagtgcagg cgattttata aaacagttta ctgtcatcct gcctcaaatg
    24661 tgaggcctta cgataataaa atcttgcctg ggcatgattt ctcatttttt catagcaata
    24721 accaatagcc tcaaaaatta catcttccgg cttggccagt tcattaactt tttccaacgc
    24781 ctcaattgct tctttatact tcctcaggcg gatataagca tcgcccatat tccggtaggc
    24841 ataatcaaat ttctcttcaa tcgtaattgc atatttatat gcatcaattg ctttttcata
    24901 caattttaat ccctggtaag ctgctgccag gttaaaccac gctaattcat tgtatgggaa
    24961 atcatctatt attttcaggt gtagccttat accctcttca ttacgaccgg taaaatctgt
    25021 ccaaaaacat atcttgtata atgcctcttc attattcggg tcgtcatcta aaatcagctt
    25081 aagacaatca aaaactttgt caaaatcctc gtaatcatca tatacatcgg ctaactcaaa
    25141 caacaactca attttttcct caccttcaaa atgggaaagg gcttcctcaa gaagctttac
    25201 cgccttctgt tgctggtcca atgccaggaa ggcatcagtt tttaaaatat aaaggttaat
    25261 gtcgccactg tcaagcagag atgcctgatc cagtgtatcc agggcctcct ggtaacggcg
    25321 atttgccagt aaaatatcgg ctttctttat cagcaacatg gctgagtaag ggaattgttc
    25381 gcttgcaatt tcagctgcct ccatcgccct gatcaggtct tctttctcct ggtaataatc
    25441 aattattttt tcgaaagcgt cttcctctat aaaagagtgg cttcggccgg ttttaagatt
    25501 ctcaaattgt ttaagcagtt cctgtatcag ttccctgtct tcccggtatg gattttcttg
    25561 catgaattcc ggtgatttag tgagcaaaaa cttttaaaga gcctgtccag acagcaaaat
    25621 ccctttgccc cctgaaatca attatttcca acaagttaaa ttaaaaaaat tggctgaaaa
    25681 aggggttagt tatccccaga tgtgcaaaat accgcaattt tagggcaacc agttgtaaca
    25741 aaatgccatc ttatgcgtta atgaagtatt aaagttctgg ataaacggtt ttttttatac
    25801 atttttttac ctttattttg tagaaatgaa tctctcaaaa ctgaccatat tatggaaaaa
    25861 taatagcagg aaactagctg ccataatttt gctggcttgt tctggtatgg gagcatttgc
    25921 aaccctgggt gacggcaata aaaaatcaaa ccttcctaaa aaatcattac tctccaataa
    25981 gacagtactg aaaccaggtt ctttttcgct ccggtcagga tataattacc gtggcagtaa
    26041 tgttatcaat actgaaaacg aaaagaaata tattagttta aatacaatag ttaccgttca
    26101 aaaaggtaaa acaaccttta ttgttcctct taagaaaaaa gttatcctta acaatttgaa
    26161 aattgatatt ggtaaccgtc aattacaaag gaactaatta atttctccct cctttacttt
    26221 cctcataagt cattattctg aaaccggatt tgggcaaatc aaactttgct tgctgtaccg
    26281 ggttaaaatt aatacttgat accgtatagg ttaccagcat gcttcccaat gttgcttcat
    26341 attgcatagc aaggccagga agattcttgt tcaggtattg aaaatccttg tttacaggta
    26401 ccaggtcttt tgtaaaatat acattgaagg tgctgccatc tgtaagttta cctatggctt
    26461 tctggcattt ataccctgca attgttttat aatcttctgc ataggtgaat gcaataccct
    26521 catatttctt attagactct ttccagttct cctgtgtcat tctaatcatg tacttctggt
    26581 caccgtactc tttcagcaca gtcacatcac cggttttacc atctacaata gtagcctgtg
    26641 tacccaatga acttaccata tcagatcggc ttaacgctcc cttcaggtaa ataacactgg
    26701 tcgctccatc aagcaaatca gcagcctgtg gtgttttatt actggtattt atcacaatat
    26761 cataagagat agtagcttct gtcaaacgtc tttgtgcctg catttcaaaa gaaaccacca
    26821 gcaagaaaac agctataaat acgatgtata tctttttcat tttagttgaa ctattagtat
    26881 ataataaaag acgtgccaat ttagccaata agttgcttaa atagaaaaac cgggtggcca
    26941 aaggcagttc ccggttcttc ttaagactat tttaactaaa aatcagcgtt tgttcttttg
    27001 ctgcatttct ttcatctttt tctgctgatc ctgcatttgc tccatccgct cctgccactt
    27061 ggatttggtc ttgggcttct tgcgattttc ctggattttg gccaaaatct tatcatggtc
    27121 aattatataa ttctgaatta caaactgcag ggccagtgta actacgtttg agacagtata
    27181 ataccaggtc aaagctgaag gaagcttatt aaaaaagaac aatagaaata ccgggaaaat
    27241 gtatggcagg tatttcatta cgggattgtt ctgatccggt gtcatactca tactgtatac
    27301 agaaataagg aaactcgtag ctgctgcagt gacgttaaat aaactaaggt ggtcgcccaa
    27361 caatggaatg ttaaagccaa atttgattgg tgcgtcatat gcagaaaggt cattcgccca
    27421 taaaaactct gcccctctta aactcacttc tgagttaaag aaactaaata aggcaaagaa
    27481 aatagggatt tgcaataaag ccggaataca accacccaaa ggatttacac cagcttcacg
    27541 gaaaagcttc atctgttcca tactgatagc ctgcttgtcc tcgccgaact tgtctttaag
    27601 tttggcaatc tccggacgaa gtgctttcat cttggccccg ctcaaataac ttgaataagt
    27661 aagtggagaa ataagcagcc gtatgaataa agtaagtaac gcaatggcaa gaccaaggct
    27721 ggtaccacta agattcctca taaaatcaaa aatcgggaga atgacaaacc tgtttaacgg
    27781 ccgaacgaag gcatacatac cctggcccag gtttacaagt ttttccagat tcagatcata
    27841 ctttttaaga atatgataat ctgatgggcc ataatagata ctaaatccgg tacttgatgt
    27901 ggaagctacg ggtaactgca ctttcatgtt ggcggtggaa cgcactaata tctttgcact
    27961 atccggtggc accgtccatt ccattttacc attggtgaaa ttatcctttg ctattaatga
    28021 tgtgaaaaag aaacgttgcc ggacacccac ccatttcact gattcatcaa agtccttact
    28081 gcttctatta ccaacagtat aatagtcaaa actaccatct ttaacaaacc caacctgtgt
    28141 attttgtttt tcaaagtcaa tgtcagattc ctgttgagct gctgcatact gccatgacag
    28201 attcatggag ccttgtgtta ataacttgtc tgcacctacc aattctacat taaaatcaat
    28261 catgtagtcc tttggcttga tagtaaactt gtgagtaatg gaggaggaag ccgtactatc
    28321 tgaagatttt aatataaacc agatgctttt actcccatca gcgttttttg cagaatcaat
    28381 gcctgtaaaa tataaatcag ccgtttgggc tgtttgattt gtggcagtat taatagtata
    28441 accgagcctg tcaaaatctg ttgcggcgag ttgaagtaag ttgctgtcct ggcccttgaa
    28501 atctttgagt tccaccctct tcacctgccc gcctttgctg gtaaaagtca gtttcaggta
    28561 ttcattctca gtataaacca gttgctccgt tccattcgct gcatccctga aactgcctgc
    28621 actggcaata cgtttcatcg tatcagccct ggcagagtca attttttgtg ctagtttatc
    28681 ctcaggtggt atattagctt ttgcaattga atccttaaca aactcttcct tagccttttg
    28741 tttgcggtaa gcagattgtt cctggttggt gtaataaaaa tagccaaaga aaagtgcagc
    28801 cagtatcaca aaaccaataa cggtattacg gtcgaacttc attcgtagta agaatttaaa
    28861 gacggcaaag atagggtaaa cagaccggag ctctttcgtt gaatttagag ggtatttact
    28921 ccgccacctg tcccctgtat ttaaatcatt tcactctgta aaaggggatt tttaacctca
    28981 cttttttttt cggcgaatgt tttggcagct tttacaaaat gaacaaaaat aggctgtgga
    29041 ttttccacgg tactttttaa ttccgggtgg tattgtaccc ctacgaagaa aggatgattg
    29101 ggcagttcga taatctccac caggccagtt ccgggatttt tcccgctggc tatcattccg
    29161 gctttttcaa acagctccag gtatttatta ttgaactccc agcggtgacg atgcctttca
    29221 ctgatactgc ttgcaccata gatttttgaa gccagtgaac cttctcttat ttcacagggg
    29281 taggaaccca gtctcattgt accgcctttg gaagtcactt ttttctgttc ttccatcagg
    29341 tctataaccg gttcttttgt atcaggattc atttctgtgg aatgagcatc ttttaaccct
    29401 aatacattcc tcgcaaactc aatagcagcc atttgcatcc ccaagcaaat accgaaaaaa
    29461 ggcatattgt tttctctggc atatttaacc gctactattt ttccttctac gccacgatga
    29521 ccaaaaccgg gtgctaccag taaaccgtcc agtcctgcta atttttcacc tacattatcg
    29581 tctgtaataa attcgctatg cacatttaca atatccactt tacactcatt gatagctccc
    29641 gcatgaataa aggattcaag gatagattta taagcatcct gtaattcaat atattttccg
    29701 ataagtccga ttgtcacttt gcctttagga tattttaatt tatccaaaaa ctctttccat
    29761 tttgtcagct caggctcttt gtattcagta atattcatct tacgcagcac tatcttatcc
    29821 agcccttccc gcatcattaa tacagggact tcatagatgg tggcagcatc aattgattct
    29881 atgacagctt cttgctttac gttgcagaac aaagcgattt ttcttcttat ttctgttgat
    29941 aatgttcttt cagtacggca aacaatcaca tcggggtgca ccccttcctg gcttagcatt
    30001 ctaacactgt gctgtgtagg cttggtcttt aattcctttg ctgctttcag gtaaggtatc
    30061 agggtgaggt gaacaactac cgaatcttcc tccggtaatt cccattgtaa ctggcgaaca
    30121 gcttccacaa aaggcaaact ttctatatca ccaacggttc cgcctatttc tgtaattacg
    30181 atatcatatt ccccggtttg cccgagcaat tgcattcttc gctttatttc atctgtaata
    30241 tgcggtacta cctgaactgt tttacctaaa taagctcctt ctctttcttt attgatgacc
    30301 gtttggtaaa tgcgacctgt ggttacgtta ttggcctgcg aagtaaaaat gttaaggaac
    30361 ctttcataat gcccaagatc caggtcggtt tcagcaccat cctccgtaac aaaacattcg
    30421 ccatgctcgt atggattcaa tgttccgggg tcaacattaa tatagggatc aaatttttgt
    30481 attgtaaccc gcagccccct tgcctgtaat agtttggcaa gcgaagctgc aataatgccc
    30541 tttccaagag aagaagtaac cccaccggta acaaagatgt acttagccat tgtatggtca
    30601 gttttatttt taataattca ggggaggaat aaataaaaac attggttaat tctggacacc
    30661 agaattactc aaaaaaaatg ataccaataa attaaagact gaaaatttgc cccaccctgt
    30721 tgaccatcgg aagtctgcaa taagagaaaa atccgggagg tcgggtaaag ttaaaagaaa
    30781 ttttccgggc ataaaaattc aataaaaggg atgtggaaaa aagccggtat tttagtgagg
    30841 aaataaaacc aacatgaaaa aatcatcact tcttgtattt gtattgttga ttacactcaa
    30901 ccttttttcg caaaaaacat atatctggtg cggcaccctg gttgacgggg tctcggaaga
    30961 gcctaaaaaa aatatgacaa ttgtagtgga gaagaataag atcatcactg ttcagaacgg
    31021 tttcaccaca gccgggactt ctgataaaac aattgacctt aaaaccaaaa ccgttactcc
    31081 cggctggata gatatgcatg tgcatatgga atttgaaacc aaccccaacc gctatgtgga
    31141 agtctttaca tcaaatcctg ccgattatgc ttttcaatct gttaagtatg ctgaggttac
    31201 attaatggcc ggttttacta ccgtcaggga tcttggaggt tccggcgtaa atatttcttt
    31261 acgaaatgcc atcaataagg gaataataaa aggtccccgg gtatacacgg caggaaagtc
    31321 aatagccaca actggcgggc atgcagatcc aacaaatgga tacagaaagg aactgatggg
    31381 tgatcccgga cctgctgaag gtgtagcaaa cggagcagaa gaatgccggc aggccgttag
    31441 acagcgatat aaagatggtt cagaccttat aaaaatcacc gcaaccgggg gagtactcag
    31501 tgtggccaaa agcggcaaga acccacaatt ttttgaagat gaaatagacg ctatcgtgaa
    31561 gacagcaaaa gactacggtt ttaaagtggc cgttcatgct cacggggaag aaggtatgaa
    31621 aagagctatc agggccggag tgaactccat tgagcatggc acttttatgg atgatgaagc
    31681 cattgaatta ttcaaaaaac atggcacctg gtatgtacct accatcactg ccggaaaagc
    31741 tgtcggcgac tcagctaaaa ttccgggtta ttatccccca gtagtagtac caaaggcttt
    31801 agagacaggt ccacagattc aaaaaacttt tgccaaagcc tataaggccg gcgttaagat
    31861 cgctttcgga actgatgcag gagtatataa acatggaatg aactggctcg agtttggtta
    31921 tatgatagaa gcgggtatga ctccaatgga tgccattaga tctgccacaa tcagtgccgc
    31981 tgatctattg ggtgaaaaag ataaacttgg gagtatagaa gccggtaaac ttgctgatat
    32041 cgttgcagtt gacggagatc cgttgaaaga tgcaaagtcc tttggtaaag tagtgtttgt
    32101 gatgaaggat gggatggtct ataaacaaca ataaactcaa tccttcaata tcttattata
    32161 actagccacc aatcctctta tcaatgatga actgaaaccc tggtgttcca tttcattaag
    32221 tccggcaata gtacaaccct tcggggtggt tactttatca atctcatgtt ccgggtgaga
    32281 gcccttctct aacaataatt gtgctgctcc tttaactgtt tgtgctgcaa ttattgaagc
    32341 ggtggccgca tcaaaaccaa tctcaatacc accctgtata ttggcccgga tataacgcat
    32401 ggcatacgct gtgccacaag cacccaacac tgtggcggca tccatcagct tttcatctat
    32461 catcactgtt tttcccaact gatcaaacaa ttgctgggta taatttagtt gtgaagccga
    32521 tgctccagca tgtgacaggc aggtcatgct ttgctgaatg gcgatagccg tatttggcat
    32581 agccctgata acaggcactg gtttaccgat tgctttatgc aattgctcga tccacacacc
    32641 tgtgatgaca gatatcacaa tgtgcttttt aggatttaat aatggcttaa tttctttaac
    32701 aatatcagca tagttataag gcttcaccgc caggatgatc acctcactgt cacgaacagc
    32761 cttcttgttg tcactatgca ccatacagcc cctttctgca aaacggctca atgttgaaag
    32821 attacgacgg gtaattgata attcaacggg acggctgaat tcactcaaga tcaatccatc
    32881 tgcaatagat gcacctaaat ttccaccacc tataattgct attttttttg ccatttctct
    32941 tcttttggta aagataagca tgtactcaaa atattatttg tttaaactat actcatgtat
    33001 cgcaaaaaag actaaacaac caataaaaga tcttattttt tctcgaaaag ctaaaacctt
    33061 ttccggcaat tgagtgttta attgaccaaa taaaaacata ttatgaaaaa gaaattcttc
    33121 tcccttttca ccgcagttgt acttagctca tccattttta tcgcctgtaa caatgaagca
    33181 gaaacaaaaa ctgctgaaga cagcacaacc gtttcaaaag aagagccaat ggctccaccg
    33241 gctgaagccc caaaagatat tgttgatgta gccgctgcct ctgctgatca cagtacatta
    33301 gtagctgctg taaaagcagc aggcctggta gaaaccttaa aaggtgccgg tcctttcact
    33361 gtatttgcgc caaccaatgc tgcttttaat gcattacctg ctggtacagt tgatggttta
    33421 ttaaagcccg aaaacaaagt agacttaacc agcattttaa cctaccatgt tgttgccggt
    33481 gccgtaaaag cagctgactt gaaagacggc cagaaagtaa aaactgttca gggtgcagaa
    33541 ttaacagtat ctatcaaaga tggtaaagtt atgattgatg gcgcaaatgt aactgcggca
    33601 gatcttgcag caagcaatgg agtgatccat gtaattgatg cagtagttac gccaaagaaa
    33661 taaggcgctt tttcattgtt gagatatata aaaaggagtg acttttgttg ctcctttttc
    33721 attaataata gttcctcaat aagaggaatt tttttacata gtcaccaaca ccttcctcca
    33781 atgtataaaa gctatccttg tatcctgcat tgcggagttt gttcatatct gcttcggtga
    33841 aatactggta tttatcccgg atatcttcag gtatgtcgat atattcaatg gcaggttctt
    33901 tcccaattcc tgcaaaagtt gcttttacca ggtcttcaaa agtccttgct ttgcctgtgc
    33961 cgagattata taatcctgat atgatggtat gctccatcaa ccaatagcaa acctttgcca
    34021 catcctccac ataaatgaaa tcgcggagtt gctgaccatc tttaaaatcc ggccggtgag
    34081 acctgaacag cttaactttt cctgttgatt ttatctggtt aaaagagtga aatatcacac
    34141 tggccatcct gcctttatga tattcattcg gaccataaac attgaagaat tttagtcctg
    34201 cccaaaaagg agggtgactt gcagtatggc cctcagtttg ctgaaggacc cacttatcaa
    34261 actcattttt ggaaacacca taaggattta gtgggtgtaa tctttcgaca agcccatgat
    34321 catctttata acccaactca ccacctccgt aagtagcagc agacgatgca taaaccagtg
    34381 ggatatttct ttctgtacaa tagttccaga tattttttga atactccaca ttcaattttt
    34441 catgaatggt gtaatcaaat tcagttgtat ctgttcttgc acccaggtga aatacgaaat
    34501 caatttcggg ttcttctctt ttcaaccaat caaacagttc ttccctttct acctttgcaa
    34561 taaaatcttt ggaatcgaag ttgaaccact tatcctcctc actaaaatca tcaacaataa
    34621 taatattctt atatccctgc ttattcaaat aaccgaggag gtaactgcca ataaaacctg
    34681 cagcacctgt cagtaatatt actttattct tttccattat attgaactca aagccgaacc
    34741 tgcttcctct tttgataaat aattctcaat tgacgaatca tatttttctt tccagtcttc
    34801 gatatgcccc cactccatac catccacttc cactgagcca tctgttacca gccccagttc
    34861 ttcaaaggaa tgattgccca ttactttttt aacaacatcc agtttttctt ttttaacaga
    34921 taccaccacc ctgctttgtt tctcaccaaa ccattgcgca tcctttctta tattatagtc
    34981 attggctacc acatcaaagc caagggagtt attaaaagat gactccagca acgtaacaaa
    35041 caatccccct tcacttacat catgggcaga ttcaatcagt cctttactga ttaattcagt
    35101 tatcttttgc tgcattgtaa attcttcttc caggtcaaaa tggggtgccg gcgaaaattc
    35161 caccccgcat attttactca ggtattgaga agaattaata tcatcagaag acgagccaat
    35221 caggagtata gcatctccca cttctttaaa atccaatgtc atttttttat taacatcttc
    35281 cagcaggccc accataccaa ttgttggcgt aggataaacg ggaccgtcag gattttgatt
    35341 ataaaaacta acgttaccgc cggtaactgg tgtatcaaac ttacggcatg cctctcccat
    35401 gcctttaata gcatggacaa actggtaata cacttccgga tcgtaaggat tgccaaagtt
    35461 caaacagttg gtaacaccta
//
LOCUS       DAORTI010000020        34895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_782_length_34895_cov_7.313806, whole genome shotgun sequence.
ACCESSION   DAORTI010000020 DAORTI010000000
VERSION     DAORTI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 34895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34895
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_34895_cov_7.313806"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLO70_04620"
     CDS             complement(<1..633)
                     /locus_tag="PLO70_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQZ73771.1"
                     /translation="MSLELEQPTFQEQFEAVIKTEDKLQIRDFLDDQNISDVAQLVDE
                     FPDYESQIIANMSVHRAASVFKILDLSFQKRIIHELPANTTASLLNELPADDRTDFLE
                     ELPSNVVRELIKLLDPEERKITLSLLGYPENSIGRLMTPDYVYVYPYNTIEEVFTTIR
                     KYGKDSETINVIYIINEKGELIDDIRIRDIILSPPDKKVTELIDERFIALH"
     gene            752..1135
                     /locus_tag="PLO70_04625"
     CDS             752..1135
                     /locus_tag="PLO70_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQZ73772.1"
                     /translation="MILPFLFIAHTEKMGKGVFTAEDIDAGTVIEISPVIIMGLQERK
                     LLDQTLLHDYIFEWGDTKEQCCMALGYVAVYNHSYQSNCEYEMDYMQELISIRTVRKI
                     VAGEELFINYNGDWNDAATVWFDTK"
     gene            complement(1190..1690)
                     /locus_tag="PLO70_04630"
     CDS             complement(1190..1690)
                     /locus_tag="PLO70_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HQZ73773.1"
                     /translation="MKLIKLTALPLLLLSFLLVITSCEPDAELKKITDFQKTAIPLTG
                     AQQAPPSASPALGSMDVFYTKETRILTYTINWSGLSGAVTGFGIHGLAPTGFASPTLV
                     QTFSTSAIVRCPTVSITSCGTYKGTLLVDGAVVKEQDLLNGMYYVNIRTAAYPAGEIR
                     GQIKFQ"
     gene            complement(1778..4408)
                     /gene="mutS"
                     /locus_tag="PLO70_04635"
     CDS             complement(1778..4408)
                     /gene="mutS"
                     /locus_tag="PLO70_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQZ73774.1"
                     /translation="MAKTASSDTPMMQQHRAIKQKYPDAILLFRVGDFYETFGQDAVL
                     ASQVLGITLTKRNNGAAASSELAGFPHHALDTYLHKLVKAGHRVAICDQLEDPKLAKG
                     IVKRGVTDMVTPGTTVNDKLLEHHSNNFLAAVHFADTSTGAQYEQYGLAFLDISTGEF
                     FIAEGDREYADKLLQSFKPAEVVFQRHQQKKFKEYFNSKIYTYTLDEWIFDSSYAEDT
                     LLKHFQTHSLKGFGVEELKNGLIAAGAIIHYLKDTEHPNLQHIISLQRIDRDDFLWMD
                     RFTIRNLELVYGNSEGNHTLLSILDNTVSPMGARLLKRWIVFPLKDIQKINERLDTVE
                     YLIKETDLRNKISLHIKQCGDIERLVSKIPMKKINPREVLQLARGLKQADAVKQICLS
                     SANEYLKRLGDALNPCPYIAEKIFKEIVENPPALAAKGGMMNSGVSNELDELRKIAFS
                     GKEYLTQLQLKEAERTGIGSLKIGFNNVFGYYLEVTNSQKNKVPAEWMRKQTLANAER
                     YITPELKEYEEKITGAEEKILKIEGELYEALLNELFDYLAPVQTNGNLLAIMDCLCCF
                     AHNAIQYQYKKPQLHTGDEWIIKDGRHPVIERNLPVGEPYISNDLELNKAEQQIIILT
                     GPNMSGKSALLRQTALITLMAHTGSFVPAAEARISLTDKIFTRVGASDNLSGGESTFM
                     VEMNETASIINNITGRSLILLDEIGRGTSTYDGISIAWSIAEHLHNSPHQPKTLFATH
                     YHELNELEDKFPRVKNYHVTNKEVGNKIIFLRKLARGGSTHSFGIHVAKMAGMPPSLI
                     DRANEILKHLESIHESGKAENMDEKIKGISAPKMQLSIFDAHSETFDEIRKLLEGTDI
                     NRLTPVEALLKLQEIKSKLK"
     gene            4501..6219
                     /locus_tag="PLO70_04640"
     CDS             4501..6219
                     /locus_tag="PLO70_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQZ73775.1"
                     /translation="MKKFLLLAGIVLSCAVSNAQNTPSEKWDTVRTDAGMNNATVYFG
                     YGAELIHESKVKMDASTRIIVINQLSTNVDINSLQVSVPEDVALLSQRYSVFYPVPPP
                     VVKVKEVERLEDSILLVQKEISRLDNLIVIDQEILTKTGLLIETTINKTNEKPVTSDE
                     LLKLLEYYTAKIEKSKTNIYNNKQTINQLNVRIAEIRKRIAGITAVTPVKQRPYGQVM
                     LQVMCKRSGEIPVALSYYTTNAGWTAMYDVRVNSKTNKVKMVYKASLTQTTGIDWKKT
                     KLTLSTGTPNFGVSAPVLTPWYLQLYVPGLYTDLQRRAAQGNAARNIIQSYAEDEKLA
                     EVVVTDAAGQYKVKTLKDQTIDPSTLQQYTTLTQGQLNTSFEIDLPYDIASDGQLNSV
                     TIKDQEIDCTLKNYAVPRMDKDAYLLAEVADWQNLDLLPGDANIIMDDTYIGKSVIDP
                     NTTADTLNLSLGRDKRIAVKRSLVKEQSSLKTSGGNTKQVFTYEILVKNNKVTDVNLL
                     LKDQFPLSNIKEVVVTLEDGSDAMINTETGVLTWKIHLKPGESKKVRFSYSVKYPKDK
                     KIVNLK"
     gene            6312..6857
                     /locus_tag="PLO70_04645"
     CDS             6312..6857
                     /locus_tag="PLO70_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQZ73776.1"
                     /translation="MRKLSMEELGRKSVEEFKASDKTPIIVVLENIRSAYNVGSVFRT
                     SDAFLVEAIYIIGYSAKPPHKEIKKTALGAEETVDWKYFKTTAEAIEELRAKGFNVFA
                     AEQAEGSYKLNAIGFEPDEKIAVVFGNEVTGVEQSTIALCDGCLEIPQLGMKHSLNIA
                     TAAGVVLWELIRGRIMLNDKL"
     gene            6865..7854
                     /locus_tag="PLO70_04650"
     CDS             6865..7854
                     /locus_tag="PLO70_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HQZ73777.1"
                     /translation="MSKIKSYLSLIKFSHTIFAMPFALIGFFVALIKIDYATSVVPGW
                     HTKIYPPSWLKDDNSIGFSKDSLLGKIFAGSNPTYIVIVYLTLVILCMVFARSAAMAF
                     NRYLDRQYDAENPRTAVREIPAGVISAKNALIFTVISSLLFIACTYFINKLCFYLSPV
                     ALVVVLGYSYTKRFTPLCHLILGLGLSLAPIGAYLAVTGQFALLPILFSLAVIFWVSG
                     FDIIYALQDEAFDRSQQLYSIPAWLGKVKALRISELLHLCSAAAVVYAGIYGHFGWLY
                     WAGTVVFIGMLLYQHSIVKPTDLKRVNLAFMTANGIASVVFAIFVIADLFINS"
     gene            7859..8272
                     /gene="ruvX"
                     /locus_tag="PLO70_04655"
     CDS             7859..8272
                     /gene="ruvX"
                     /locus_tag="PLO70_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQZ73778.1"
                     /translation="MPRIICIDYGQKRTGIAVTDPLQIIATGLTTIHPKELIPFLKDY
                     FSKEPVELILIGWPTNWDDSATHVTALVEKCIKDLQKHFPEMAIKKVDERYTSKMAKD
                     AMLEMGLKKMQRRNKALVDEIAATIMLQEYMRAIG"
     gene            8300..8878
                     /gene="def"
                     /locus_tag="PLO70_04660"
     CDS             8300..8878
                     /gene="def"
                     /locus_tag="PLO70_04660"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQZ73779.1"
                     /translation="MILPIVAYGHPVLRKLATDITSGYPQLDKFIADMWETMYASSGV
                     GLAAPQVNKDIRLFVVDTLQMFNGMNEEEQKEYPDAPGIKEVFINAHIAELEGDEWPY
                     NEGCLSIPKIREDIYRQESVTLHYCDEHFQELTKTFTGLSARVILHEYDHIEGKLFID
                     HISPLKRKLIKGKLNDISKGKVNVDYKMMFPG"
     gene            8884..9822
                     /locus_tag="PLO70_04665"
     CDS             8884..9822
                     /locus_tag="PLO70_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73780.1"
                     /translation="MKHLLLLLLFIVNCFLSVAQEEDSLDDQRLAKMISLSEVIIRTD
                     LNVARFINQVKNDTTFYKAFRNLHLLGFTAWNDIRIKDKKGNIKAALMSKTKQTRANG
                     CRSMDILNENTSGDFYEGDGGYNYYTAELYAGLFFTKGSVCGETNIVKGIDFRPRSQK
                     GLAKHKEQLKMLFFNPGKKIPGIPFIGDKIDIFDPERAAYYDFLIDYGDYEGQSCYVF
                     SIKAKKELGGKTDKIVIDSMVTWFNTRTMEVMARNYDMSYNAGVYDFDIHMEVEMTKF
                     GDYLVPKLLRYIGNWDVAFKKRERGLFTATLFDFTN"
     gene            complement(9823..10884)
                     /locus_tag="PLO70_04670"
     CDS             complement(9823..10884)
                     /locus_tag="PLO70_04670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQZ73781.1"
                     /translation="MQNELENENPEIQDSSDELYERFTITIDKGQEPLRIDKFLTNRI
                     EGATRNKLQQAINLGMVMVNGASVRPNYKIKPLDSIIVYSDMSPDETDVVPEKMDLNI
                     VYEDADLMIINKPAGMVVHPGSGNYSGTLLNGVSYYLQQQNPAISEDILPRFGLVHRI
                     DKNTSGLLVLAKTDKAMRQLAKQFFDHTVKRQYVALVWGDMEKDAGTIIAHVGRNLRF
                     RKLFEAYPEGDHGKDATTHYKVIERFGYVTLVQCILETGRTHQIRVHMKYIGHPLFND
                     DFYGGDKIVKGTVYSRYKQFVDNCFQICQRQALHAKTLGFTHPTSGKEMFFDTELPED
                     MEKVIEKWRKYASTTGNKK"
     gene            10935..11642
                     /gene="lipB"
                     /locus_tag="PLO70_04675"
     CDS             10935..11642
                     /gene="lipB"
                     /locus_tag="PLO70_04675"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQZ73782.1"
                     /translation="MRKEQIIFQDLGKMPYQAAWDYQEKLLQENVKRKSTVGGQQLTV
                     NHLLFVEHPPVYTLGKSGNMDNVLMSDDELQANNIEFFHTNRGGDITFHGPEQIVGYP
                     ILDLEKFYTDIGKYLRNIEEVIILTMAAYGIKGDRSAGETGVWIEPGVPGKERKICAI
                     GVRCSRWITMHGFAFNVNTDLSYFNNIIPCGIVNKQVTSMQKELGHSLDMQEVKEKVK
                     SNFERVFEAELISEVLL"
     gene            complement(11625..12395)
                     /locus_tag="PLO70_04680"
     CDS             complement(11625..12395)
                     /locus_tag="PLO70_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73783.1"
                     /translation="MKKLTLFFFFSALSITGSIAYSQDTLPKFSLKNVGKNRIVISWV
                     NNFQNIRQLSIQRSFDSLKGYKTILTVADPTSLQNGYVDGKATNDHMFYRLYIMLEGG
                     VYLFSKAKKPVLDTATAPKTEITSVVDSLLRVDSVTRVNPVVFRIDNLQPGDSATIPS
                     PVTIKNRPTAFVPSLYIYTHKDGYVSVNLPVEEKTKKYNIKFFEEDETPIFELKDIKE
                     RNFKIDKVNFYHAGWFRFELYEDGKLIEKHKFYLEKDF"
     gene            complement(12467..13357)
                     /locus_tag="PLO70_04685"
     CDS             complement(12467..13357)
                     /locus_tag="PLO70_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQZ73784.1"
                     /translation="MEDINLNNITTDMLSTPLFEKRQLDVSILRLDKIHPLISGNKWF
                     KLRFHIDEAKKQGKKKILTFGGAWSNHILATAAACKLYNLFAIGIIRGEKAAALSPTL
                     IQAKELGMQLVFTSREEYKKKMIPNELNDEDCYIVNEGGFGARGTVGASSILAHCKKN
                     EYNHICCAVGTGTMMAGLIISAVTGTKVTGISVLKNNTELQDQVTTLLTAKMENYEII
                     HEYHFGGYAKHKPELLAFMNSFYRQTSVPTDFVYTGKLCFAVNDLAEKNFFPPESRIL
                     VIHSGGLQGNQSLNKGTLIF"
     gene            13420..14316
                     /locus_tag="PLO70_04690"
     CDS             13420..14316
                     /locus_tag="PLO70_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQZ73785.1"
                     /translation="MKPTLVILAAGMASRYGSMKQVQGFGPSGETIMDYSIYDAIRAG
                     FGKVVFIIRKEFADNFRAIFEPKLKGKIETEYVYQGMGSFTNGFEAPQERTKPWGTAH
                     AVLCAKEVVTEPFAVINADDFYGRNAFENAADFLNNSCTDKCYAIIGYDLLSTLSDHG
                     TVNRGVCGLDDMGNLATITERINIEKKEGKIVCNDGLEPVELSTATRVSMNFWCFAPS
                     FFSYTQKLFIEFLEKYGTELKSEFFIPIVADQFIKEGNKVEIIPTTSLWFGVTYKEDA
                     PFVEKSLNELISKGEYPESLWG"
     gene            complement(14389..15492)
                     /locus_tag="PLO70_04695"
     CDS             complement(14389..15492)
                     /locus_tag="PLO70_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQZ73786.1"
                     /translation="MGDIKVLEAVERYIRGEMNPDERLHFENLRKTNPEIDQLVVEHT
                     LFLQQMNRFGEWKKFRSSLNDVHTDLAEQGKIDSARLNGKAKVVYLWKRYKRVAAIAA
                     SIAGITTLTFSALVWSITPQTPASEIDLLKGKINVIENKTKEQDKEINKVKNKINTAD
                     NTIVYTTGGTGFIIDAKGYLVTNAHVVEGAKNIAVQNSRGEYMAKVVHINMEKDIAIL
                     KIDDESFKPYASVPYTISRSAGKLAEPIFTLGYPRSEIVYGQGYLSAKTGFNGDTLSC
                     QLEIAANRGNSGSPILNKKGEVIGILNGRQRNIEGFAFAIQGKYIYNVLDELKKDSIH
                     QKVKISSKSSIAGLDFQQQVEKVEDYVFMVKVN"
     gene            complement(15485..16060)
                     /locus_tag="PLO70_04700"
     CDS             complement(15485..16060)
                     /locus_tag="PLO70_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ73787.1"
                     /translation="MNSENNEKALLQGLATNDKKAVETIYRENYNMVQYLIINNNGSA
                     DDAKDIFQEAMIVLYEKVRSGTFELNCQIKTYVYSVSKRLWLKKLQQSSRYSPPIEDQ
                     ETSVPVDDEIAEHEKRDAEFEMMDKAISSLGEPCKSLLEAYYLQKQNMQVIAANFGYT
                     NADNAKNQKYKCLMRLKKIFFAHYKNENGDG"
     gene            16201..16398
                     /locus_tag="PLO70_04705"
     CDS             16201..16398
                     /locus_tag="PLO70_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73788.1"
                     /translation="MKKFFAIAFIAATTLVACNNSGEGEKPADTTAVPAVDTTVVAPV
                     VDSTVKAIDSTVKAVVDSIKK"
     gene            16560..18245
                     /locus_tag="PLO70_04710"
     CDS             16560..18245
                     /locus_tag="PLO70_04710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQZ73789.1"
                     /translation="MNTFEALGIEEGLLNSIKVLGFTQPTPIQEKAIPVLLQGTKDFI
                     GLAQTGTGKTAAFGLPLLQLINKEDRFPQALIVCPTRELCLQITSDLEKFKNKKTNVH
                     VTAVYGGTSITMQIRDLKKGTHIVVATPGRLIDLIERKAINLEKIHYVVLDEADEMLN
                     MGFKEDIEFILKNTPNRQSTWLFSATMPPEIRQVSKRYMDKPFEITIGKTNEGAANID
                     HQYYATQHHNRYEVLKRIIDFNPGIYGIVFARTKADTQDIAERLTREGYDIDAIHGDL
                     TQQQRDKVMGLFRDKSIQLLIATDVAARGIDVTGITHVINYELPDDVEVYTHRSGRTG
                     RAGKSGVSVSIVTPKEVYRLRQIERILNARVHKMDIPSGKDVCRKQFFHFIDKMLQAD
                     ISHGEYETYLPILKEKFEAVEKEEILQRVAALEFDRFLKYYENAADLNMRTEDRRTER
                     RGERVERGDRPASRDRDTKGRSFSSGGNYQRLFVNLGTKDGFYKASFLQFILDMSDLK
                     KEVLGRIDMKEMTSWIEIEPSAGKKMIKAIDGKNYRGRKIRMNDAESGGGRRK"
     gene            18451..19281
                     /locus_tag="PLO70_04715"
     CDS             18451..19281
                     /locus_tag="PLO70_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQZ73790.1"
                     /translation="MSTKVTIQGYEGSFHQVAAQLFHGRNVEVIPCDTFRDVVKIASN
                     KKESDGGVMAIENSIAGSILPNYNLLQKSNLKIIGEIYLQIRQNLLVNPGVKLEDIKE
                     VHSHTMALQQCYGFLDKYKWKLVETDDTALSAKHIHQHKSKHVAAIASKLAAELYDLN
                     IIAPNIHTLKNNYTRFLFLQREGEAINIENANKASVNFHTDHSRGSLAKVLTKIAEGK
                     INLSKLQSFPIPGSDFKYSFHADMEFDSATQFNKVIEVIRPMTAAIKIYGVYKRGLWK
                     "
     gene            19272..20426
                     /locus_tag="PLO70_04720"
     CDS             19272..20426
                     /locus_tag="PLO70_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ73791.1"
                     /translation="MEVAKRLNGIGEYYFSQKLREIDELNKAGKNVINLGIGSPDLPP
                     HPEVISVLQEETARHNVHAYQSYKGSAVLRRAMGHWYKNWYNVELDPESEILPLIGSK
                     EGIMHICMTYLNKGDEVLVPNPGYPTYRSAVKLAGGKCTEYKLKERNNYEPDFEKLGK
                     IKLKKVKLMFVNYPQMPTGQLPTMELFEKLVAFGRKHEILIIHDNPYSFILNENPVSL
                     LSAKGAKDCVIELNSLSKSHNMAGWRVGMLCGARKRIDEVLRFKSNMDSGMFLPVQLA
                     AARALSLGKEWHEEVNAVYRKRRAKVLGLLDLLGCKYSKEQAGMFIWASIPSAYKNGF
                     VLSDEVLYNAGVFITPGGIFGDAGEKYIRVSLCSTEDKFEEAIQRIKKCK"
     gene            20428..21276
                     /locus_tag="PLO70_04725"
     CDS             20428..21276
                     /locus_tag="PLO70_04725"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HQZ73792.1"
                     /translation="MERKKIAIVGVGLIGGSLAIQLHEKKLSSRLIGVEVNEEHAKKA
                     MELELVDEIMSLEDAVGAAEVIVLAIPVDKVLSLLPAIMDKIDQQVVIDLGSTKRQSI
                     ELVKNHTKRGRYVATHPMWGTEYSGPQAAVRGAFENKAVIICNAEESDADALDWVKGM
                     YKKIGMHLLEMEAKAHDLHAAYVSHISHITSFALANTVLEKEKEEDAIFELASAGFES
                     TVRLAKSNPAMWVPIFMQNKENVLDVLKEHIAQLQRFKESIEKNDDTYLMKLIDDANK
                     IRRILK"
     gene            21273..21731
                     /locus_tag="PLO70_04730"
     CDS             21273..21731
                     /locus_tag="PLO70_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ73793.1"
                     /translation="MIFREALVSDIRKMQVVRNAVKENVLSNPALITDAVVEEYITKK
                     GKGWVCEIDNLIVGFAIADIVDHNIWALFVDPAFEKKGIGRQLHQLMMNWYFNQTTET
                     VWLSTSPGTRAETFYRQQGWNETGVYGKVEIKFEMTKARFRFLMNRIIQS"
     gene            21820..22908
                     /locus_tag="PLO70_04735"
     CDS             21820..22908
                     /locus_tag="PLO70_04735"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQZ73794.1"
                     /translation="MQSAEQTTNEIVQAAWDKRPLIISGPCSAETEDQVLATAQRLAA
                     TGKVDMLRAGIWKPRTKPGMFEGVGAKGLPWLQQAKKLTGLPTTVEVATGKQVQDALT
                     FDVDVLWIGARTTVNPFSVQEVADALRGVDIPVMIKNPINPDLELWSGAVERIARAGI
                     RQIGLIHRGFSSYGNSEYRNAPMWHLAIDMKLKHPGMPLINDPSHICGRREILLEVMQ
                     KAIDLDYDGLMIESHIDPDNAWSDAKQQVTPERLAEMLDSIIWRKEDVNSEEYHAALE
                     ALRQKINHLDDELMQILGQRMKIAEQIGKYKKENNITILQTSRWNEIIERASVKGDKI
                     GLSKEFITKYLDAVHMESINHQKKVLDS"
     gene            23044..24063
                     /gene="aroB"
                     /locus_tag="PLO70_04740"
     CDS             23044..24063
                     /gene="aroB"
                     /locus_tag="PLO70_04740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQZ73795.1"
                     /translation="MKHINYKFSGSSTDFYFAYGISHLKKITDPNATVLITDENVYKA
                     HTKRFRGWNTIVLKPGEEFKVQATADALIEKLIDMEADRKTTLVGVGGGVITDITGYV
                     ASVYMRGLRFGFIPTSILALVDASIGGKNGLDVGVYKNMVGVIRQPSFILHDMVFLNS
                     LPQQEWENGFAEIIKHACIKDAVMFAELERNSLKTYQGRQKSICEIIRKNALIKTRVV
                     QKDEFEKGERRLLNFGHTLGHALETQYELLHGQAISIGMTYACHISAQLAGFKQTEKV
                     IAVLEKYNLPTYASFDKQKVFDVLKMDKKRERKEMNYVLLERIGKGVVKSISLVQLGK
                     IIQDL"
     gene            24141..25475
                     /gene="aroA"
                     /locus_tag="PLO70_04745"
     CDS             24141..25475
                     /gene="aroA"
                     /locus_tag="PLO70_04745"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQZ73796.1"
                     /translation="MKVTIQPSQLKGSIQTPASKSSMQRALAAGLLTKGKCIINNPGH
                     SNDDKAALKVIKAMGAAIDSTGDQLIIESNGVNPLTNEVNCGESGLGIRMFAPIIAVS
                     EKEITIHGEGSLLDRPMDFFDEIFPQLGVKIKSNHGKLPIVIKGPLQPANIEVDGSLS
                     SQFLTGLLMVYGASTNSFAGEALDSGNSIVSIKVRDLKSKPYIDLTLDVMKQFGLRVP
                     ENKNYEEFIFHTDSSLFPPVGSGRAVYTVEGDWSGGAFLLVAGAIAGPITVRGLDLTS
                     TQADRAIVGALMAANAGIAMDAKGIQLHPTPMDGFYFDATDCPDLFPPLVALAAYCKG
                     KTTIKGVSRLLHKESNRALTLQDEFDKMGVTIELNGDLMVIHGGGVVKGADVHSHHDH
                     RIAMACAVAGLKSDSEMVIEDAQAVNKSYPDFYNDLKSLGASVSLPHQHFNL"
     gene            25487..26479
                     /locus_tag="PLO70_04750"
     CDS             25487..26479
                     /locus_tag="PLO70_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQZ73797.1"
                     /translation="MNSFGRIFRISIFGESHGECAGITIDGCPAGLSLAAEDLLPDLE
                     RRKGGIQKGTTPRQEEDYPIFMSGVFNGKTTGFPVTILFENKNIRSEDYNKQRSIPRP
                     GHADWVANQKFGGNEDYRGGGHFSARLTTGIVAAGVIAKKLMKDVSIQADVTEIGGEK
                     DLEKGLQKAIDARDSVGGIVECRVKGLPVGLGEPYFDSLESVLSHFLFAIPAAKGVEF
                     GSGFAAARMFGSLHNDAIENKEGKTRTNHAGGIVGGISNGNELVFRLAVKPTSSTPKE
                     QNSLNWDTGKTENFSIKGRHDLCVALRAPVIVEAVAAIVIADFMLLEQKIKRVL"
     gene            26479..26778
                     /locus_tag="PLO70_04755"
     CDS             26479..26778
                     /locus_tag="PLO70_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HQZ73798.1"
                     /translation="MPVIYHVTKAADWNVAKKNGFYEHPSLNDEGFIHCSQANQVAGV
                     LERYFAGQTDLVKLVIDTDKLTSKFVFDWSPSTQDTFPHVYGPINVDAIIDVIAL"
     gene            26971..28689
                     /locus_tag="PLO70_04760"
     CDS             26971..28689
                     /locus_tag="PLO70_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQZ73799.1"
                     /translation="MNFWKPPHHNMMKRIAYPFISSFIFYFIAASCNGKTGKGQDTIA
                     GSPEELQQKTTELIRSAAESAFAKKGILADSAITIGQPNLTRMLYAKNEYTPIWSAKQ
                     TWLPFGDSLFDFIEQSQLYGLFPEDYHAIQLKEIRTKFYIDSLTKNNKKDAALWAKAD
                     IMLTDAFVRIVKDLKLGRLPNDSVTLRKDSVLSDEFYLQQLNELQKSGVTRVVQSLEP
                     DQRGYRLLKEGIKAFLATASYRQFTKVPYPEKDKQKFNRLLQKRLYEEGFLSFDSITA
                     DSAQLAAAVKLFQNKKGITVDGRAGEGTIRMLNINDRERFIRIAISMDKYKLLPERMP
                     SKYIWVNAAGNLLELIDGDKVKITSRVICGKPKTRTPVLSSVISSLITYPQWVPPPSI
                     VGKEILPAVKKNPGYLARKGFSLLDKNGDEVDPYTVDWSRYGNNIPYRIVQGSGDANA
                     LGIMKFVFDNKYSVYLHDTNQRYLFANAMRSLSHGCVRVQEWEKLAFYILQNESMDAG
                     SGAAKIDSVKTWLKYKQKRSIPIRNKIPVYIRYITCAGQNGNIVFFDDVYGEDKMLRV
                     RFFAGK"
     gene            28845..30179
                     /locus_tag="PLO70_04765"
     CDS             28845..30179
                     /locus_tag="PLO70_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ73800.1"
                     /translation="MKKIISAFIALYLLVPAARAQDDEIRPKALGVSFFLNDFVSPER
                     IRTTSLSQVLSDKSMAKIKEMSPGLAVTYFKGIRKHIDIAASLGAAFLRYPMPGKSFA
                     DNNLLLEGNATINLKMTTEKYWVQPYLIAGVGAHKYRSYYGAFLPVGLGLKVNFLDEA
                     HLFVTSTYRVPVTNETANYHLQHSIGIAGRLGKKKEPVVIEPPKPPADTDGDGIIDSL
                     DKCPTVKGLAKYDGCPIPDTDKDGINDEEDKCPTVPGLARYQGCPIPDTDKDGINDEE
                     DKCPTVAGVARYQGCPIPDTDGDGVNDEEDKCVTIPGPRENFGCPVISEEVKKKVNMA
                     ANNILFVTGSAKLQTKSFKGLNEVAQILKDNPGMSLNIDGHTDNVGSDEKNQVLSDNR
                     ANSVKAYLVSKGVEESRIAAQGYGETKPIADNKTAAGRQKNRRSELTLSYYK"
     gene            30356..32500
                     /gene="fusA"
                     /locus_tag="PLO70_04770"
     CDS             30356..32500
                     /gene="fusA"
                     /locus_tag="PLO70_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQZ73801.1"
                     /translation="MADLKFQRNFGIAAHIDAGKTTTTERILRYTGMIHRIGEVHDGA
                     ATTDWMEQEKERGITITSAAVSCQWNFPTVLGKATPETKKYSFNIIDTPGHVDFTVEV
                     ERSMRVLDGLIALFSAVDGVEPQSETVWRQANRYGVPRIGFVNKMDRAGADFLMVVKQ
                     VKEMLGSKAVPLQLPIGAEDDFKGVVDLIKMKGIIWHMETEGMTFDEIEVPADMLEEA
                     KEWRASLVEAVAEYDDKLMEKFFDDPNSITEDEMHEAIRKATVDLSIVPMMCGSSFKN
                     KGVQTALDAVCRYLPSPIDIPDTIGTNPDTGEQISRKADPKEPFAALAFKIMTDPFVG
                     RLAFFRCYSGHLDAGSYVLNVRSGKKERISRIMKMFANKQNPIDFIEAGDIGAAVGFK
                     EIKTGDTLCDENHPITLENMFIPEPVIAVAVEPKTQADVDKMGMAIAKLVEEDPTLRV
                     NTDEDTGQTILRGMGELHLEIIIDRMRREFKVEVNQGAPQVAYKEAFQATVEHRETLK
                     KQTGGRGKFADIQFSLGPIDAEWKTENPDKNYQFVNDLFGGSIPREFVPAIQKGFEQS
                     MTTGVLANYPVDNMKIRVFDGSFHAVDSDSMSFELCAKQGFREAARKAKPVLLEPIMR
                     VEVITPAQYMGDVTGDLNRRRGMLEGMDTRAGAEVIKAKVPLSEMFGYVTQLRSLSSG
                     RASSTMEFSHYSPAPTNIAEEVIAKVKGKVSA"
     gene            complement(32699..33604)
                     /locus_tag="PLO70_04775"
     CDS             complement(32699..33604)
                     /locus_tag="PLO70_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQZ73802.1"
                     /translation="MGKSKNTQSKTNKVLKLLLKVIITVVCLWYVSGKIDFAKAGDAL
                     KNTSWFYLFAALFLFIFSKIISAKRLNIYFKNINVHLPEWENIKLYWLGMFYNLFLPG
                     SISGDAYKVIILTKKYNVPYQKTTAAVLLDRFSGLLGLGLVLATYSIITLDNNLYITF
                     LVIGSLLAVQASYFIIKRYFKDFISSFAPTLLLGIIVQASQVVCVYLIMAALKIPAHS
                     TEYIFIFLISSIVSVLPLTIGGLGIREVVFLEGSEYFGLSQETSVVISILFYLITLFT
                     SACGMYYVFKSPLKEKGFSDEPLIS"
     gene            33640..>34895
                     /locus_tag="PLO70_04780"
     CDS             33640..>34895
                     /locus_tag="PLO70_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQZ73803.1"
                     /translation="MIKRILPFILFVFCAGMCYGQEKPLVAGMKILRTMKIKKAVYQI
                     EAPADTAKPVLFIEGDNITIDFNNAEMNGSNRNKNPEEFSGVAILIRNSKNVTIRNLK
                     ARGYKIALLARNVEKLTLDNCDFSYNYRQHLNSTQEKEDVSDWMSYHHNENDEWLRYG
                     AAMYLRGCNFVTIKNCKVTGGQNALMLMECNDGMIYNNDFSFNSGIGIGMYQSNRNSI
                     MYNRVVFNVRGYSHGVYNRGQDSAGMLVYEQSSNNIFYKNNVTHGGDGFFLWAGQTTM
                     DNGKGGCNDNIIMSNDFSYAPTNGIEATFSRNTILSNKIFECDHGIWGGYSYNTTIAE
                     NQFRNNRIAIAIEHGQYNNFHHNLFIKDKEAVRLWARPQQPENWGYAKYRNTRSVGYV
                     MSSNSFNDHQLVYNITRTDSLNIFDNT"
BASE COUNT        10692 a         6331 c         7752 g        10120 t
ORIGIN      
        1 gtggagggca ataaatcttt cgtcaattaa ttcagtcact ttcttatcag gcgggcttag
       61 tataatatcc cggatgcgta tatcatcaat caattcacct ttttcattga taatatagat
      121 gacatttatt gtttcactgt cctttccata tttacgtatc gtagtgaata cttcttcgat
      181 agtgttataa ggataaacat atacatagtc aggagtcatt aagcgaccaa tgctattttc
      241 aggataaccc agtaatgaaa gggtgatttt tctttcttca gggtcaagta gtttgatcag
      301 ttctctgacc acattactgg gtaattcttc caaaaaatcg gtgcggtcgt ctgcaggcag
      361 ttcatttaat agcgaggcgg tggtattggc gggcagttca tgtataattc ttttctgaaa
      421 tgagaggtca aggattttaa atacactggc agcccggtgc acagacatat tggcaatgat
      481 ctggctttca tagtcaggaa actcatcgac taattgagcc acatcactga tattctggtc
      541 atccaggaaa tcccttattt gaagtttgtc ttctgtttta atgactgctt caaattgttc
      601 ttgaaaagtg ggttgttcca gttctaatga catgtttcaa aagtacgaag gagaaagtta
      661 cctgcctatc ctataaaatt tggtttacat cagagaaggg gctttttatt aagaaaaata
      721 cgtacttcgt tatttttaaa tcttacttga catgatcctc ccgtttcttt ttattgcaca
      781 caccgaaaaa atgggtaaag gagtgtttac agctgaagat attgatgccg gtacagttat
      841 tgaaatttca ccggttatta ttatgggtct gcaggaaagg aaactgctcg accagacatt
      901 actccatgac tatatttttg aatggggcga tacaaaggag caatgttgta tggcgcttgg
      961 ctatgtagct gtttataatc attcttacca aagcaattgc gaatatgaaa tggattatat
     1021 gcaggagcta ataagtatca ggaccgtacg aaaaatagta gctggtgagg agttgtttat
     1081 taactataac ggcgattgga atgacgctgc aacagtctgg tttgatacga aataaaaaaa
     1141 ccgtcttacc cgggcaagac ggttatgaaa taattttaaa aaagtttatt tattgaaatt
     1201 tgatttgccc tctaatttcg cctgccgggt aagcagctgt acgaatattt acatagtaca
     1261 ttccattcaa taaatcctgt tctttaacaa cagcaccatc aacgagcagg gtgcctttat
     1321 acgttccaca ggaagtgata cttacagtag gacacctgac aatggctgaa gtggaaaagg
     1381 tttgaacaag tgttggagaa gcaaagccgg ttggtgccaa accatgtatg ccaaaaccag
     1441 taactgcacc agatagaccg gaccaattta tggtgtaagt caatatcctg gtttctttgg
     1501 tataaaaaac atccatagaa ccgagagcag gtgaggctga tggcggtgct tgctgggcgc
     1561 cggtcaatgg tattgcggtt ttctgaaaat ccgtaatttt tttcagttca gcatctggtt
     1621 cacaagatgt aattacgagt aaaaatgaaa gtaaaagcag gggtaaagca gttagtttaa
     1681 tcaatttcat gattttcggt atttgtggta aaaataagaa gtttttctaa atggcaatac
     1741 ttttcaataa ataggtaaaa ccctgtgagt aaactgttta tttgagctta ctttttattt
     1801 cctgcagttt gagcaaagct tccaccgggg tcagccggtt gatatcagtt ccctccagca
     1861 attttcggat ctcgtcaaat gtttcactat gggcatcaaa aatggaaagc tgcatttttg
     1921 gggctgatat tccttttatt ttttcatcca tattttctgc ctttcccgat tcatggatac
     1981 tctccaaatg ttttagtatt tcattcgccc ggtcaatcaa agacgggggc atcccggcca
     2041 ttttagccac atgtatacca aaactatggg tactgccgcc acgggccagt ttgcgcagaa
     2101 aaatgatctt attccccact tctttattgg tgacatgata attttttact ctcggaaact
     2161 tatcctccag ttcattcaat tcatgatagt gggtggcaaa cagcgttttc ggctgatgcg
     2221 gcgagttatg taaatgttca gcaatactcc aggcaatgga aataccatca taagtggaag
     2281 tgcctcttcc tatttcatct aacagaatca agctcctgcc tgtaatgtta ttaatgattg
     2341 aagcagtttc attcatctcc accataaaag tggactcgcc gccggataaa ttatcagaag
     2401 ctccgaccct ggtgaatatc ttatccgtta atgatatcct ggcttcagct gcgggaacaa
     2461 aagaaccagt atgtgccatg agtgtaatca gcgccgtttg ccgcagcagg gcggatttac
     2521 cactcatatt tggcccggtc agtataatta tctgttgttc tgctttattc aattcaagat
     2581 cgttgctgat atatggttct ccaaccggga ggtttctttc aattacagga tgccgtccat
     2641 cttttatgat ccattcatcg ccggtgtgca attgtggttt tttatactga tactggatgg
     2701 cattgtgcgc aaagcagcag aggcaatcca taatggccag caggttgccg ttggtctgta
     2761 ctggtgccag ataatcaaaa agctcattca gcaatgcttc atataactcc ccttcaatct
     2821 tcaaaatctt ttcttcggct cccgtgattt tttcttcgta ttcttttaat tcgggagtaa
     2881 tgtaacgttc agcattggcc agtgtctgct tgcgcatcca ttcagccggc actttatttt
     2941 tttgggagtt ggtcacttcc aggtaataac cgaaaacatt attaaatcca atcttcagcg
     3001 atccgatacc tgttctttct gcttctttta attgcagttg cgttaaatat tcttttccac
     3061 tgaatgcaat tttcctgagt tcatccagtt cattgcttac tccgctgttc atcatacccc
     3121 ctttagcagc aagggccgga ggattctcaa cgatttcttt aaatattttt tctgctatat
     3181 aaggacaggg attcagtgca tcgcccagtc gtttcaggta ttcattggct gatgataagc
     3241 atatttgttt tactgcatct gcctgtttca ggccccttgc cagttgcaat acctctctgg
     3301 gattgatttt tttcataggg atcttggaaa ccaacctttc gatatctcca cattgtttga
     3361 tgtgcaggct gatcttgttc cgcagatccg tttcttttat caggtattca actgtgtcta
     3421 accgttcatt tattttctgg atatctttta acgggaaaac aatccaccgc ttcagcaacc
     3481 gggcacccat tggtgaaacg gtattgtcta agatgctgag cagcgtatga tttccttcag
     3541 agttaccata cactaattca aggttccgga tggtaaaccg gtccatccat aagaaatcat
     3601 cacgatctat ccgttgtaat gaaataatgt gctgcaggtt gggatgttcg gtatccttta
     3661 aataatggat aatggctccg gctgcaatca aaccattttt cagttcttca acaccaaatc
     3721 cctttaagga atgggtttga aaatgtttca gtagtgtatc ttctgcataa gatgaatcaa
     3781 aaatccattc atctaaagta tatgtgtata tcttactatt aaaatattct ttgaatttct
     3841 tttgctggtg ccgctggaaa acaacttctg caggcttaaa actttgcagc aatttatcag
     3901 catattcccg gtcgccttcg gcaataaaaa attcccccgt tgaaatatca agaaaagcca
     3961 atccgtattg ttcgtactga gcaccggtcg aagtatctgc aaaatgaaca gctgctaaaa
     4021 aattattgct gtgatgctcg agcaatttat catttacagt ggtaccgggg gtcaccatat
     4081 ctgtcactcc tctttttaca atgcccttgg cgagttttgg atcttctaat tggtcgcaaa
     4141 tagccacccg gtgaccagcc ttaaccagtt tgtgcagata ggtatccagg gcatggtggg
     4201 ggaatcctgc caattctgat gaagctgccg ccccgttatt tcttttggtt aaagtaatgc
     4261 ccagtacctg tgaagccagc accgcatcct gtccgaaggt ttcataaaaa tctcctactc
     4321 tgaacagcaa gatagcatcg gggtatttct gcttgatggc ccggtgctgc tgcatcattg
     4381 gtgtatcgga actcgctgtt ttcgccattg catgtcccgg tttatcggga gacgacaaaa
     4441 ataaggaagt gtttatgtaa gattttctct ttctgcatct gttaaaaaat aaaatttgcc
     4501 atgaagaaat ttctcctgtt agccgggata gtactttcct gtgccgtatc aaacgcccaa
     4561 aacactccaa gcgaaaaatg ggatacagta agaaccgatg ccggtatgaa taacgctact
     4621 gtttattttg gctacggcgc cgaactgatc catgaatcca aagtcaaaat ggatgcgtcc
     4681 accagaataa ttgttatcaa ccagcttagt accaatgtgg atataaacag cctgcaggta
     4741 agtgtgcctg aagatgttgc gcttttatca caacggtaca gtgtatttta tccagtacca
     4801 ccacctgtag taaaagtaaa agaagtggaa agattggaag acagtatcct gctggtgcaa
     4861 aaagagatca gccggttgga taatttgatt gtgattgacc aggaaattct gaccaaaaca
     4921 ggattactga tagaaactac tatcaataaa acaaatgaaa agcctgttac cagcgatgaa
     4981 ttactgaagt tgctggaata ctacacagcc aaaattgaaa aatcaaagac caatatttat
     5041 aataataaac aaaccatcaa ccaattaaat gtccgcattg cagagatccg gaaaaggatt
     5101 gctggcatta ctgcagtgac tcctgttaag caaagaccgt atggccaggt aatgttgcag
     5161 gtgatgtgta agcgttcagg tgaaattcct gttgcgctgt cttattatac taccaatgca
     5221 ggatggaccg ccatgtatga tgtaagggta aattcaaaga ccaataaagt gaaaatggtg
     5281 tacaaagcat cattgaccca aaccactggt attgactgga agaaaacaaa gctcacctta
     5341 agtacaggaa ctcccaactt tggtgtatcc gcccctgttc ttacaccctg gtatcttcaa
     5401 ctatatgtgc cgggtctcta cacggacctg caaaggaggg cagcgcaggg caatgcagct
     5461 agaaatataa ttcagtctta tgcagaagat gaaaaacttg ccgaagtagt ggtaacagat
     5521 gcggccgggc agtataaagt aaaaacactg aaggatcaaa ccattgaccc aagcacactg
     5581 cagcagtata ccacccttac acagggacaa ttgaatacca gttttgaaat tgatttgcct
     5641 tatgatattg ccagtgatgg ccagttgaac agtgtaacga ttaaggacca ggagattgat
     5701 tgcacactga aaaactatgc cgtaccaaga atggataaag atgcctacct gctggccgaa
     5761 gttgccgact ggcaaaatct tgacctgctg cccggcgatg cgaatatcat catggatgat
     5821 acctatattg gaaaatcggt aattgatccc aatactacgg ctgacacctt aaatctttca
     5881 ttgggcagag acaagcggat tgcggttaaa cgttctttgg tgaaagagca aagcagctta
     5941 aaaacaagcg gtggtaatac aaaacaggtg tttacctatg agatactggt gaagaataac
     6001 aaagtgacag atgtgaattt attactgaag gaccagtttc cgcttagtaa tatcaaagag
     6061 gtggttgtaa cgctggaaga tggcagtgat gccatgataa ataccgaaac cggtgtgctt
     6121 acctggaaga tccacctgaa acccggggaa agtaaaaaag taagattcag ctatagtgtg
     6181 aaatatccga aggataagaa gattgtgaac ctgaaatagc ggttcaattt gtacataaaa
     6241 gttaattggt tgattgggag attttacccg gttaaccaat taacttttta actaatccct
     6301 acttttgcgc catgcgaaaa ctgagcatgg aggaactggg gcgaaaatcc gtggaggagt
     6361 tcaaagcttc tgataaaact cccattattg tcgtactgga aaatatccgc agtgcttata
     6421 atgtaggcag tgtattccgt acttcagacg ctttcctggt agaagccatt tatataatag
     6481 gctattcagc taaacctcct cataaagaaa taaagaaaac ggccctcgga gctgaagaaa
     6541 cagtggactg gaagtatttt aaaacaactg cagaagctat tgaagaattg agggccaaag
     6601 gttttaatgt atttgcagcc gaacaggctg aaggcagtta taaactgaat gcgatcggtt
     6661 ttgaacctga tgaaaaaatt gctgttgttt ttggaaacga agtaacaggg gtagaacaat
     6721 ccacgatcgc tttatgtgat ggctgtttag agattcctca gttaggaatg aagcattccc
     6781 tgaatattgc aaccgcagcg ggagtagtat tgtgggaatt gatccggggc agaattatgt
     6841 tgaatgataa attgtaaata ttgaatgagt aaaataaaaa gttatctaag cctgattaaa
     6901 ttctcccata ccatcttcgc gatgccattt gcgttgattg gttttttcgt ggcgctgata
     6961 aagatagatt atgcaacatc ggtagttccg ggctggcata ccaaaattta tccaccctca
     7021 tggttgaaag atgataattc aattggattt agtaaggatt cattgttagg aaaaatattt
     7081 gcaggtagca atccaaccta catagtaata gtctatctca ctttagttat cctctgtatg
     7141 gtctttgcac gcagcgcagc tatggcgttc aatcgttatc tcgacaggca atatgatgct
     7201 gaaaatccaa ggactgcggt aagagaaata ccggcagggg ttatttctgc aaagaatgca
     7261 ttaatcttta ctgttatcag cagcttgctt tttattgcct gcacctattt tataaataag
     7321 ttgtgttttt atttatcacc tgttgcgctg gtagtggtgc ttggttatag ttacaccaaa
     7381 cgatttaccc cgctttgtca tttgatcctt ggtcttggtt tatccctggc accgattggg
     7441 gcctacctcg ccgttacagg tcagtttgca ttattgccca ttcttttttc gcttgctgtt
     7501 atattctggg tcagtggctt tgatattatc tatgccctgc aggatgaagc gtttgataga
     7561 tcgcaacaac tttattctat tccagcctgg ctgggtaaag taaaagcact gcggatttct
     7621 gaattgcttc atttatgtag tgcggctgct gttgtttatg caggtattta cggtcatttt
     7681 ggatggctgt attgggcagg cacggttgta tttattggta tgctgctcta tcagcattca
     7741 attgtgaagc caactgatct gaaaagagtt aaccttgctt ttatgaccgc caatggtatt
     7801 gcttcagtag ttttcgccat ctttgttata gcagatttat ttatcaactc ataatccatt
     7861 gccaagaatt atttgtatag actacggaca gaaaagaacc ggcattgccg ttacagaccc
     7921 cttgcagatc attgccacgg gtcttactac cattcatccg aaagagttga ttccttttct
     7981 gaaagactat ttttcaaagg aaccggtaga actgatttta attggctggc ctaccaactg
     8041 ggatgattct gctacccatg tcactgcatt ggtagagaaa tgcattaagg acctgcaaaa
     8101 gcattttccg gaaatggcca ttaaaaaagt ggatgagcgc tatacttcca aaatggccaa
     8161 agatgctatg ctggaaatgg ggctcaaaaa aatgcagcgc cggaacaagg ccctggtgga
     8221 cgaaatcgct gccaccatca tgttgcagga atatatgcgg gcaattggtt aattgcaccg
     8281 gtcagggtaa atttgcaaaa tgattttacc tattgtagca tacggtcacc cggttcttag
     8341 aaaattagcc accgatatta catccggtta tcctcagttg gataaattca ttgcagatat
     8401 gtgggaaacc atgtatgcca gcagtggggt tggcctggcg gccccgcagg tgaataaaga
     8461 tatccgtctg tttgttgtcg atacactaca gatgtttaat ggcatgaatg aagaggagca
     8521 aaaggaatac ccggatgccc cgggaataaa agaagtgttc attaatgcac atattgcaga
     8581 actggagggt gatgaatggc catataatga aggatgctta agtattccaa agatcaggga
     8641 agatatttac cggcaggaga gtgtgacact gcattattgt gatgaacatt ttcaggagct
     8701 gacgaaaaca tttactggtc tttctgccag ggttatactc catgaatacg atcatattga
     8761 agggaaactt tttattgatc atatttcacc gttgaaaaga aaattaataa aaggcaaact
     8821 caatgatatt tctaaaggca aagtgaatgt ggactacaag atgatgttcc ccggctgatc
     8881 attatgaagc atctcctcct cttattacta tttatagtaa actgttttct ttctgttgca
     8941 caggaagaag attctcttga tgaccagcga ctggccaaaa tgatcagtct ttccgaagtt
     9001 attatccgta cagatctgaa tgtggccagg ttcattaacc aggtaaagaa tgacactacg
     9061 ttttataaag cctttcgcaa cctgcacctg ctgggattta cagcctggaa tgatatacga
     9121 ataaaagata aaaaggggaa tattaaagca gccctgatga gtaagacaaa gcaaacaagg
     9181 gctaatggct gccggtctat ggatatattg aatgaaaata ccagcggtga tttttatgag
     9241 ggcgatggcg gttataatta ttataccgca gaactgtatg cagggttatt ttttactaaa
     9301 ggttcagttt gcggggaaac caatattgta aaaggaattg atttcaggcc ccgttcacaa
     9361 aaagggttgg cgaaacacaa agaacaactc aaaatgcttt tttttaatcc tggtaaaaaa
     9421 atacccggca tccctttcat aggagacaaa attgacatat tcgatccgga aagggcagcg
     9481 tattatgact ttttaattga ttacggggat tatgaaggtc agtcctgtta tgttttttca
     9541 atcaaagcga aaaaggaatt agggggcaaa acagataaga ttgttattga cagcatggtt
     9601 acctggttta atacaaggac aatggaagta atggcccgta attatgacat gagttataat
     9661 gccggtgtat atgattttga catccacatg gaagtggaga tgacaaaatt tggtgattat
     9721 cttgttccta aattactccg gtacataggc aactgggacg tggcttttaa gaaaagggaa
     9781 agaggtttgt ttacggcaac gctttttgat ttcaccaact aactattttt tattaccagt
     9841 tgtagaagcg tactttctcc atttttcaat caccttttcc atatcctccg gaagttctgt
     9901 atcaaaaaac atttctttgc cacttgtcgg gtgagtaaaa cccaacgttt tggcatgcag
     9961 tgcctgtcgc tgacatatct ggaaacagtt gtctacaaat tgtttatacc tgctgtatac
    10021 tgttcctttt acaattttat cacctccgta aaaatcatcg ttgaaaaggg ggtgcccaat
    10081 atatttcata tgcaccctga tctggtgggt gcgtccggtt tccagaatac attgtaccag
    10141 ggtaacataa ccaaatcttt cgatcacttt gtaatgtgtc gtagcatctt tcccatgatc
    10201 gccttccggg taagcttcaa atagttttct aaatcgcaga ttacggccca catgggcaat
    10261 aatagttcct gcgtcttttt ccatatctcc ccacaccaat gctacatatt gtctttttac
    10321 tgtatgatca aagaattgtt tggccagttg tcgcatggcc ttatctgttt tggcaaggac
    10381 caaaaggcca cttgtgtttt tgtcaatccg gtgaaccagg ccaaatcttg gcagaatgtc
    10441 ttcagaaata gcaggatttt gttgttgtaa ataataagaa actccattta aaagcgtacc
    10501 cgaataatta ccacttcccg ggtgtaccac catacctgcc ggtttattga taatcatcag
    10561 atctgcatcc tcatatacaa tattcaaatc cattttttcc ggaacaacat ctgtttcgtc
    10621 cgggctcata tctgaataaa ctataataga atcgagcggc ttaattttat agtttggccg
    10681 cacggaagct ccatttacca ttaccattcc taagttaata gcctgctgaa gtttgttacg
    10741 ggtagcgcct tcaatccggt tggtcagaaa tttatcaatc cgaagcggtt cctggccttt
    10801 atctatagtg atagtgaacc gttcatagag ttcatcacta ctatcctgta tttcgggatt
    10861 ttcattttcc aattcgtttt gcataagcgg caaaggtaaa ggatggtttt ttgttggtaa
    10921 atttgctttt ctatatgcgt aaagaacaga taatatttca agacctggga aagatgccat
    10981 accaggcagc ctgggattac caggagaagc tattacagga aaatgtgaag agaaagtcaa
    11041 cagtgggggg gcaacaactc accgtaaatc atttactttt tgtggaacat ccccctgttt
    11101 atacactggg caagagtggc aacatggata atgtcctgat gagtgatgat gaattgcagg
    11161 caaacaatat tgagttcttt catacaaacc ggggaggcga tataactttt cacggccctg
    11221 aacagatcgt tggttacccg atactggacc tggaaaagtt ttatacagat atcggcaaat
    11281 acctgcggaa tattgaagag gtgatcatac tgactatggc agcatacggc ataaaagggg
    11341 acaggtcagc tggtgaaaca ggtgtatgga tagaaccagg agtgccggga aaagaaagaa
    11401 agatatgtgc tattggtgtt cgttgcagtc gctggatcac tatgcatggg ttcgcattta
    11461 atgtaaatac cgatctttct tattttaata atatcattcc ctgtggtatc gtgaacaagc
    11521 aggttacttc gatgcaaaaa gagctggggc actctttgga tatgcaggaa gtgaaagaga
    11581 aagtgaaatc aaattttgaa agagtatttg aggccgaatt gatatcagaa gtccttctct
    11641 aaataaaatt tgtgtttttc tatcagtttg ccatcttcat ataattcaaa tcgaaaccat
    11701 ccagcatgat agaaattgac tttatcaatt ttaaaatttc tttctttaat atcctttaat
    11761 tcgaagattg gtgtttcgtc ttcttcaaaa aatttaatat tgtatttctt cgttttttct
    11821 tcaacaggga gattgacact gacataacca tctttgtgag tataaatata aagcgagggt
    11881 acaaatgctg ttggtctgtt ttttattgtt accggactgg gaatggtggc agaatctccg
    11941 ggttgcaggt tatctatcct gaacactacc gggttcactc ttgtaacaga atctacccgc
    12001 agaagagaat caactaccga agtaatttca gtctttgggg cggtcgcagt atccagaaca
    12061 ggttttttgg ccttgctaaa caggtacact ccgccttcaa gcattatgta caacctgtaa
    12121 aacatatgat catttgttgc cttcccatcc acatatccgt tttgcaggga agtggggtct
    12181 gcaactgtaa ggatggtttt atatcctttt aaactgtcga atgaacgctg gatacttaat
    12241 tgccggatat tttgaaaatt attgacccag ctgattacga tccggttttt tcccacattt
    12301 ttaagtgaaa attttggcaa agtatcctgc gaatatgcaa tactgccggt gatagatagt
    12361 gcactaaaaa agaaaaatag ggtcagtttt ttcatatagt atgaatgaca aaaatatcgg
    12421 attcttttac ttatgcaaat aaagataagg cagtgatccg gaaaaattaa aatattaacg
    12481 ttcccttgtt cagtgactga ttaccttgta agccaccact gtgtatcacg agaatgcggc
    12541 tttcgggggg gaagaagttt ttttctgcca ggtcattaac ggcgaaacat aatttaccgg
    12601 tatatacaaa atcagttgga accgatgttt gccggtaaaa tgaattcata aaagcaagta
    12661 attccggttt gtgttttgca taaccgccaa agtggtattc atgaataatt tcgtaattct
    12721 ccatcttagc ggtcaataaa gttgtaacct gatcctgtaa ctcggtatta tttttaagta
    12781 cgctgatgcc agtgactttt gttcccgtga ctgcactgat gatcaagccg gccatcatag
    12841 ttcctgttcc aacagcacag caaatatgat tgtactcatt tttcttacag tgtgccagga
    12901 ttgatgaagc tcccactgtt cctctggcac caaagccacc ttcatttaca atgtaacaat
    12961 cttcatcgtt caattcattc gggatcatct tctttttata ttcttcacgg gaggtaaata
    13021 ccagttgcat gcccagttct tttgcctgaa taagtgtcgg ggaaagtgca gcagcttttt
    13081 caccccggat aattccaatt gcaaacaggt tataaagttt gcaggcagct gcagttgcca
    13141 gaatatgatt tgaccatgct ccgccaaaag tcagaatttt ttttttgcct tgtttctttg
    13201 cttcatcgat atgaaaccga agtttgaacc atttatttcc ggaaataagc ggatggattt
    13261 tatccagccg gaggatggat acatccagtt gtcttttttc aaacaacggc gttgaaagca
    13321 tatccgtagt aatgttgttt aggtttatat cttccataaa taatgtccgg ctgacaaaat
    13381 tactttaatt tcgtcctgca atttttataa acaatactaa tgaaacctac tcttgtaata
    13441 ttagctgctg gcatggcttc ccgatatgga agtatgaagc aggtacaggg ctttggccca
    13501 tcaggtgaaa cgatcatgga ctattcgatt tacgatgcaa tccgtgccgg atttggaaaa
    13561 gttgttttta ttatccgcaa agagtttgct gataatttta gagccatatt tgaaccgaaa
    13621 cttaaaggca agatcgaaac ggaatatgtt tatcagggaa tgggctcatt tacgaatgga
    13681 tttgaagcgc cgcaagaaag aaccaaaccc tggggtactg cacatgctgt actctgtgca
    13741 aaggaagttg taacagaacc ttttgcggtg atcaatgcgg atgatttcta tggcaggaat
    13801 gcttttgaaa atgcagctga ttttttaaat aattcctgta ctgacaaatg ttatgcaatt
    13861 attggttatg acctgctgag tacattatcc gatcatggaa ctgtaaacag gggagtatgt
    13921 ggcctggatg acatgggtaa cctggccacc attacagagc ggataaacat agaaaaaaaa
    13981 gaaggtaaaa ttgtttgtaa tgacggtctt gagccggttg agctgtcaac ggcaaccagg
    14041 gtttcaatga atttttggtg ttttgcacca tctttctttt catacacgca aaagctgttt
    14101 attgagttcc tggaaaaata tggaactgaa cttaaatcgg aatttttcat ccccattgta
    14161 gccgaccagt ttataaagga agggaataaa gttgaaatta tcccaaccac ctcgttgtgg
    14221 tttggtgtga cgtacaagga agatgcgccg tttgtggaga aaagcctcaa tgaattgata
    14281 agtaaaggag agtatcctga aagtctttgg gggtaaattt atttaggtga aaagttaaaa
    14341 gctatccatc atagacggat agcttttttt tatagggata gtatcttgtt agttcacttt
    14401 aaccatgaag acatagtctt ccactttctc aacctgttgc tggaagtcaa gacccgcaat
    14461 cgaggattta gaagaaattt ttactttttg atggattgaa tcttttttca gttcgtccag
    14521 tacattataa atatacttgc cctgtatagc aaaagcaaag ccttcgatgt tcctctgccg
    14581 tccattcagg atgccaatga cctcaccttt tttgttcaaa atgggactgc cgctgtttcc
    14641 cctgttggct gcaatttcta actggcagct gagtgtatct ccgttgaaac ctgtcttggc
    14701 acttaaataa ccctgaccat atacaatctc acttctgggg tagcccaatg tgaatatggg
    14761 ttctgccagt ttgcctgcag accggctgat tgtgtagggt actgaagcat atggtttgaa
    14821 actttcgtca tctattttca ggatggcaat atctttttcc atatttatgt gaaccacttt
    14881 ggccatgtat tcccctctgc tgttctggac ggcaatattt tttgcaccct ccaccacatg
    14941 ggcattagtg accaggtaac ctttggcatc aataataaaa ccagtaccgc cggtagtata
    15001 aacaattgta ttatcagccg tgttgatttt atttttaacc ttgttgatct ctttatcctg
    15061 ctcttttgtt ttattttcaa tgacgttgat tttgcctttc agcagatcga tttcgcttgc
    15121 aggtgtttgc ggtgtaattg accatacaag ggcactgaaa gttaatgttg taataccagc
    15181 aatagaagca gcaatagcgg ctactctttt atatcttttc cacagataaa caactttggc
    15241 ttttccattc agtcttgcag aatcaatctt gccctgttca gcaaggtctg tatgaacatc
    15301 attaagagag gagcggaatt ttttccattc gccaaaccgg ttcatctgct gcaaaaagag
    15361 agtatgctca accactaact ggtcaatttc aggatttgtc tttctcagat tttcaaaatg
    15421 taacctttcg tcaggattca tttcgccccg gatatagcgt tcaacagcct ctaaaacttt
    15481 aatatcaccc atcaccgttc tcgtttttat agtgagcaaa aaatattttc ttcaatctca
    15541 tcaggcactt gtacttctgg ttttttgcat tgtcggcatt tgtatacccg aaatttgccg
    15601 caataacctg catattctgc ttttgcaagt aataagcttc caaaagactt ttacaaggtt
    15661 ctccaaggct gctgatggct ttgtccatca tttcaaactc ggcatccctt ttttcatgtt
    15721 cagctatttc atcatcaaca ggtacggagg tttcctggtc ttcaatagga ggtgaatatc
    15781 ggcttgattg ttgtaacttt ttgagccaca gtcttttact tacagaataa acatatgttt
    15841 taatctggca gttcagctca aatgttccgg atctcacttt ttcgtacaac acaatcattg
    15901 cttcctgaaa tatgtctttt gcgtcgtctg ctgatccgtt gttgttgatt atcaggtact
    15961 gaaccatatt gtagttctcc ctgtaaatgg tttcaacggc ttttttgtcg ttcgtagcta
    16021 atccttgcaa aagggccttt tcgttattct ctgaattcac attccctagt ttaatacggg
    16081 aatttaaaaa tagtaaccct gcgccacctg aaaaaaaaaa ttaaaacttc gggttacctt
    16141 ttttcaattt gggtataaac aacgaaatat ttcaaacaac ccccaaaaaa tctcaaacaa
    16201 atgaagaagt tttttgcaat tgcttttatc gctgctacta ctttggtagc ttgtaacaat
    16261 tctggtgaag gtgaaaagcc tgctgataca acagctgttc ctgctgttga tactacagtt
    16321 gttgcaccag tagtagattc taccgttaaa gctattgact ctacagttaa agctgtagta
    16381 gattctatta agaagtaaga tttcaactgg caagcaaatc gctgaaggcc gttccatacc
    16441 ggggcggctt tcttattttt gtttcctctc ctctctttat atatctggca aaatacatta
    16501 gcctttcccc tttttaaagg ctacctttgc cgctttatta tcaacaaaaa agtatatcag
    16561 tgaacacatt tgaagccttg ggtattgaag agggcttgct caattcaata aaggtattag
    16621 gctttaccca gcctacacct atccaggaaa aagctattcc ggttctctta cagggtacca
    16681 aagattttat tggcctggcc caaactggaa cgggcaaaac agcggctttt ggcttgcctc
    16741 ttttacaact tataaataaa gaagatcgtt ttccgcaggc gttgattgta tgccctaccc
    16801 gggagctttg tttgcagata accagcgacc ttgaaaagtt taagaacaaa aaaacgaatg
    16861 tgcatgtaac agctgtttat gggggcactt ctattaccat gcagatacgg gatctgaaaa
    16921 aggggacgca tattgttgtg gctactccag gcagattgat cgaccttatt gaacgtaaag
    16981 caattaatct tgaaaagatt cactacgttg ttttggatga agcggatgag atgctgaaca
    17041 tgggctttaa agaagatatt gagttcattt taaaaaatac acctaaccgg cagagtacct
    17101 ggctcttcag tgcgactatg cccccggaga tacgacaagt cagcaaaagg tacatggata
    17161 agccttttga aattactatc ggaaaaacaa acgaaggggc tgccaatatc gaccatcagt
    17221 attacgctac ccagcaccac aaccggtacg aagtattaaa gcggatcatt gatttcaatc
    17281 cgggtattta tggaattgtc tttgcccgta ccaaagctga tacgcaggat attgcagaaa
    17341 gactaacaag agaagggtat gatattgatg cgatacacgg tgacctcact cagcagcaac
    17401 gggacaaagt aatgggtctt ttcagggata aaagcataca gttgctgatt gctaccgatg
    17461 tagcagcaag agggattgat gtaacgggga ttacccatgt tattaattat gaattaccag
    17521 atgatgtaga agtatatacc caccggagtg gccgtacagg ccgggctgga aaaagtggtg
    17581 tgtcagtttc tattgtaaca cctaaagaag tttatcgtct tcgtcaaatt gaaagaatac
    17641 tgaatgcaag ggttcataaa atggacatac caagcggtaa agatgtttgc cgtaaacagt
    17701 ttttccattt tatagacaaa atgttgcagg ctgatattag tcacggtgag tatgaaactt
    17761 acctgcctat acttaaagag aaatttgagg cagtagaaaa agaagaaatt ttgcagcggg
    17821 tggctgcatt agagtttgac cgttttctta agtattatga aaatgcggca gacctgaata
    17881 tgagaacaga agacagaaga actgaacgaa ggggagaaag ggtggaaaga ggtgacagac
    17941 ctgcttcaag agacagggat acaaaaggca gaagttttag tagcggagga aattaccaac
    18001 gactgtttgt aaacctgggg acaaaagatg gattttataa ggcaagcttt ctccagttta
    18061 tacttgatat gagtgatttg aagaaagaag tgctcggccg gatagatatg aaagaaatga
    18121 cgagttggat tgagattgag ccatcagcag gtaagaaaat gataaaggca attgatggga
    18181 aaaactatcg tgggcgaaaa atccgtatga atgatgctga gtcaggtggt ggcagaagga
    18241 aataatttat tggaaaattt gctatactga acaagaaagc tatatttgtt tacagatgac
    18301 agcaacaaag acatattttg gtttttactt ttattacttc ttttttaaaa tgaagccggg
    18361 aatgcttttg ttgaaaacaa aatagaacaa ctaaaaaaca acaacataaa gagtcccggc
    18421 cacaacaggc cgggactctt gttttatacg atgagcacta aagttacaat acaaggctat
    18481 gaaggcagtt ttcaccaggt ggctgcgcaa ctatttcatg gcaggaatgt ggaagtgata
    18541 ccctgcgata ctttccggga tgtagtaaag attgcatcaa ataaaaaaga aagtgatggt
    18601 ggggttatgg cgattgagaa ttctattgcc ggtagtattt tgccaaatta caacctgctg
    18661 cagaaaagca acctgaaaat tatcggagag atctatttgc aaatcagaca aaacctgctg
    18721 gtcaatccgg gagtgaagct ggaagatatt aaggaggtcc attcgcatac catggcattg
    18781 cagcaatgtt atggcttttt agataaatac aaatggaaac tggttgagac agatgatact
    18841 gcattgagtg caaaacacat ccaccagcat aaaagcaaac atgtggcagc aattgcgagt
    18901 aaactggctg cggaattata tgacttaaat attattgctc ccaatattca tactttgaaa
    18961 aataattata cccgtttcct ctttctgcaa agagagggag aagctattaa tattgaaaat
    19021 gccaataaag catctgtcaa ttttcatact gaccattcaa gagggagcct tgcaaaagtg
    19081 ctgacaaaaa ttgccgaagg gaaaattaac ctgagtaaac tacaaagttt tccaataccg
    19141 ggtagtgatt ttaagtacag ctttcatgct gatatggagt ttgattccgc aacgcagttt
    19201 aataaggtga ttgaagtaat aagaccaatg acggcagcaa ttaaaattta cggagtatat
    19261 aaaagaggat tatggaagta gcaaaacgat taaatggtat tggtgaatac tatttctcac
    19321 aaaaactgag agagatagac gaattgaata aagcaggtaa gaatgttatc aatctgggta
    19381 ttggtagtcc tgacttgcct ccgcatccgg aagtaataag tgtcttgcag gaagaaacag
    19441 cgaggcacaa tgttcatgca tatcagtcgt ataaaggttc ggctgtatta agaagggcaa
    19501 tggggcattg gtataagaat tggtataatg ttgaattgga tccggaatct gaaattttgc
    19561 cactgattgg cagtaaggag ggtatcatgc atatatgcat gacttacctt aataaaggtg
    19621 acgaagtgct tgttcccaat cccggttatc caacttatag aagtgcggtg aaattggccg
    19681 gaggaaaatg tacagagtat aaattgaagg aacggaataa ttatgaacct gattttgaaa
    19741 agctgggaaa aataaagctg aagaaagtta agctgatgtt cgttaactat ccgcaaatgc
    19801 caacaggaca gttgccgaca atggaactct ttgagaagtt agttgcattt ggcaggaagc
    19861 atgagattct gatcattcat gataacccct acagttttat tctgaatgaa aaccccgtta
    19921 gtttactgag tgcaaaaggg gccaaagatt gtgtgataga attaaactcg ttaagtaaaa
    19981 gtcataatat ggccggctgg cgggtgggaa tgctctgcgg ggctagaaaa aggattgacg
    20041 aagtgcttcg gtttaaaagc aatatggaca gtggtatgtt tttgccggtg cagctggcgg
    20101 ctgccagggc tttgtcatta ggtaaggaat ggcatgaaga agtgaatgcg gtttacagaa
    20161 agagaagggc taaagtattg ggattacttg acctgttagg ctgcaagtat tcaaaagaac
    20221 aggcaggaat gtttatttgg gcgtccatac cttcagcata caagaatggg tttgtgctaa
    20281 gtgatgaagt actatacaat gccggagtat ttattactcc cggtggaatt tttggagatg
    20341 ccggtgaaaa gtacataaga gttagtctct gtagcacaga agataaattt gaagaggcca
    20401 ttcagcggat taaaaaatgt aaataaaatg gaaaggaaaa agattgcgat agttggggtg
    20461 ggacttatcg gtggttctct tgcgattcag ttgcacgaaa agaaattatc atccaggctt
    20521 attggtgtag aggtgaatga agaacatgct aaaaaagcga tggaactgga actcgtagat
    20581 gaaataatga gcctggaaga tgcagtcgga gccgctgagg tgatagtgct ggccattccg
    20641 gttgataaag tgttgtcttt gctgcctgct ataatggata aaatagatca gcaggtagtg
    20701 attgatttag gttccacaaa aaggcagtct atcgaacttg taaaaaatca taccaagagg
    20761 ggaagatatg tggccacaca ccccatgtgg ggaacagaat acagcgggcc ccaggcagct
    20821 gttcggggtg catttgaaaa caaggcggtt attatttgca acgcagagga aagcgatgct
    20881 gatgccctgg attgggtgaa aggaatgtat aaaaaaatag gtatgcattt attggaaatg
    20941 gaagcaaaag cacatgacct gcatgccgca tatgtgagtc atatctctca cattacttca
    21001 tttgcattgg ccaacaccgt gttggaaaag gaaaaagaag aggatgccat ttttgaatta
    21061 gcatctgccg gttttgaaag caccgtccgt ttggcaaaaa gtaacccggc aatgtgggtg
    21121 cctatcttta tgcagaataa ggagaatgtt ctggatgtat taaaagaaca tattgcccag
    21181 ttacagcgtt tcaaagaaag tattgagaaa aacgacgata cctatttaat gaagctgatt
    21241 gatgatgcaa ataaaataag gaggatatta aaatgatctt tcgggaagct ttggtgagtg
    21301 atataaggaa gatgcaggtc gtccgtaatg cggtaaagga aaatgtattg agtaacccgg
    21361 cattaataac tgatgctgta gtggaagagt acattacaaa aaaaggtaaa ggttgggttt
    21421 gtgaaattga taatcttatt gtcggctttg ctattgcaga tattgttgat cataatatat
    21481 gggctttgtt tgtagacccg gcatttgaaa agaaagggat cggtagacaa ctgcatcaac
    21541 tgatgatgaa ctggtacttt aatcaaacaa cagaaacagt atggttaagc actagcccgg
    21601 gaacaagagc ggaaacattt tatcgtcagc aaggctggaa tgaaacaggt gtatacggaa
    21661 aagtagaaat aaaatttgaa atgactaaag caagattcag gtttttgatg aacagaatca
    21721 ttcaatccta agttgtgcga tccatgatat tcatcagaac aggcacaaca aatgttgagt
    21781 aaagtaaaga acgaaggcta aataaattaa atttgtcaaa tgcaatcagc agaacaaaca
    21841 acaaatgaaa tagtacaggc tgcctgggat aaacggccgt tgatcataag cgggccttgc
    21901 agtgcggaaa cggaagacca ggttttagca acagcacagc gccttgctgc aacaggtaaa
    21961 gtggatatgc tgcgggcagg aatatggaaa ccccgtacca aacccggtat gtttgagggg
    22021 gtgggagcaa agggattgcc ctggttgcaa caggcaaaga aactaactgg tctgccaaca
    22081 accgtggaag tggcaacggg caaacaggtg caggatgcat tgacctttga tgtggatgta
    22141 ttatggattg gtgccaggac aaccgttaac cctttcagtg tgcaggaagt agcagatgca
    22201 ttgcgtggtg tggatatacc ggtgatgatc aaaaatccca tcaaccctga cctggaatta
    22261 tggagcggag cagtggaaag gattgcaaga gccgggatca ggcaaatagg attgatacat
    22321 cggggtttca gttcttatgg taattcagaa taccgtaatg cacccatgtg gcacctggct
    22381 attgatatga aactgaaaca tccgggtatg ccgttgatca atgacccctc gcatatttgt
    22441 ggccgccggg aaatattact ggaggtgatg cagaaggcaa ttgaccttga ttatgatggg
    22501 ttgatgattg aaagtcatat agaccctgat aatgcatgga gtgatgccaa acagcaggta
    22561 acgccggaga gattggcaga aatgctagat agtatcatct ggcgaaaaga agatgtcaat
    22621 tctgaagaat atcatgcagc actggaagca ctgcgtcaga agatcaatca cctggatgat
    22681 gaactgatgc aaatattggg tcagcggatg aagattgcag agcaaattgg taagtataaa
    22741 aaagagaata atatcactat ccttcagacc agtcgctgga atgagatcat tgaaagagcc
    22801 agtgtcaagg gagacaagat tgggttgagc aaagaattca tcactaaata ccttgatgcc
    22861 gtacatatgg agagtattaa tcatcaaaag aaggtgttgg atagttaagt aaccggggag
    22921 ttgtgaatag tttgtatgca attttgttcc ttgttgtaat aatactatca tcctctattg
    22981 ggccagtacc tatgaatagc gggtttcgta ctcatatact ttagcaattc tatgctgcaa
    23041 aaaatgaaac atatcaatta taaattctcc ggttcatcta ctgattttta ctttgcttac
    23101 ggaatcagtc atctgaaaaa gataactgat cctaacgcaa cagtgctgat tacggatgaa
    23161 aatgtttaca aagctcacac gaaaagattc agggggtgga acactattgt gctgaaaccc
    23221 ggagaagagt ttaaagtgca ggcaaccgcc gatgcactta ttgaaaaact gattgacatg
    23281 gaagctgacc gtaagaccac attggtgggt gttggcggcg gggtgattac agatattacc
    23341 ggctatgtgg cttcagtgta catgagagga ttacgttttg gatttatacc tacttctatt
    23401 ttagcattgg tagatgcttc catcggtggt aagaatggcc ttgatgtggg agtatataaa
    23461 aacatggtag gggttatccg tcagccctcc ttcattttac acgatatggt tttcctgaat
    23521 tcactgccac aacaggaatg ggaaaacggt tttgcagaaa ttattaaaca tgcctgtata
    23581 aaagatgcgg taatgtttgc ggaactggaa agaaattcat tgaaaacata ccagggcagg
    23641 caaaaatcaa tttgcgaaat aatccggaaa aacgctctga taaaaaccag ggtggtacag
    23701 aaagatgaat ttgagaaagg ggagaggcga ctactcaatt ttggtcatac gctggggcat
    23761 gcattggaaa cccagtatga attactgcat gggcaggcta tttcaattgg tatgacctat
    23821 gcctgtcata tttcagcgca actggcgggg ttcaaacaaa cagagaaagt gatagctgtc
    23881 ttagaaaaat acaacctgcc tacctatgct tcattcgata aacaaaaagt ttttgatgtg
    23941 ctgaaaatgg ataagaaaag ggaaagaaaa gaaatgaatt atgtgctgct ggaaagaatt
    24001 ggtaagggag tagtgaaaag tatatcgttg gtgcaattag gaaaaattat tcaggattta
    24061 taaaaagctg ctgataatca tgattgttat gattttatca gcggctatct tcacaatcat
    24121 aaaaatctgc gttccggtat atgaaggtta caatacaacc gtctcaatta aaaggaagca
    24181 ttcaaacgcc ggcttccaaa agctcaatgc aaagagcttt ggcggctgga ttattaacta
    24241 aaggaaagtg catcattaat aatcccggtc atagcaatga tgataaagca gcattaaaag
    24301 taattaaagc catgggggct gccatagata gtaccgggga tcaattgata atagaaagta
    24361 atggtgttaa tcccctgaca aacgaagtaa actgcggtga aagcggctta ggcatcagga
    24421 tgtttgcgcc tatcattgca gtaagtgaaa aagaaattac tattcatggc gaaggaagtc
    24481 tgttagacag gccaatggat ttttttgatg aaatatttcc acaacttgga gttaagataa
    24541 aaagcaacca tggcaaactt ccgatagtta taaaaggtcc gttacaaccc gcaaatattg
    24601 aagtggatgg ttcgttaagt tcgcagtttc taactggttt gttgatggta tatggggcct
    24661 ccacaaactc cttcgcagga gaggctttag actccggtaa ttcaatagta tctataaagg
    24721 ttcgtgatct caaaagcaaa ccatatatag acctgacatt ggatgtaatg aaacaatttg
    24781 gtttaagagt acccgaaaat aaaaactatg aagaatttat ttttcatact gactcttctc
    24841 tatttccccc cgtgggaagt ggaagggctg tgtataccgt tgaaggcgac tggagcggag
    24901 gtgcatttct tctcgtagcc ggtgctatcg ctggtccaat tactgtaaga ggcttagatt
    24961 taacttctac ccaggctgac agagctattg ttggcgcatt aatggcagca aatgctggta
    25021 ttgccatgga tgcaaaaggg atacaattgc atccgactcc aatggatggt ttttattttg
    25081 atgcaactga ctgccccgac ctgtttcctc cgcttgtggc attagctgcc tactgcaaag
    25141 gaaagacaac tatcaaaggg gtttcaagat tacttcataa agaaagcaac cgggctttga
    25201 ccttgcagga tgagtttgat aagatgggag taacaattga actgaatgga gacttgatgg
    25261 tcatacacgg tggtggagta gtgaaaggtg cagatgtgca ttcgcatcat gatcaccgga
    25321 tagctatggc ttgtgctgtt gccggtttaa aatccgatag tgagatggtg atcgaggacg
    25381 cccaggcggt taataaatct tatcctgatt tttacaatga tttaaaatct cttggggcat
    25441 cagtatcttt gccacatcaa catttcaacc tgtaaacttt ttagctttga attcatttgg
    25501 ccgaatattc cgcatttcaa tttttggtga atcgcatgga gaatgtgccg gtataacaat
    25561 agatggttgc ccagcaggct tgtctttggc tgcggaagat ttattgcccg atctggaaag
    25621 aagaaaaggg gggatccaaa aaggaactac accaaggcag gaagaagatt acccgatatt
    25681 catgagtggt gtgtttaacg gaaaaacaac aggattccct gtcactattt tatttgaaaa
    25741 taagaatata cgcagcgaag attataataa acaaagaagt attccgcggc cgggtcatgc
    25801 tgattgggtg gcaaatcaga aatttggagg aaatgaagat taccggggtg gcggacattt
    25861 cagtgcaagg ctcacaaccg ggattgttgc ggcaggagta attgcaaaga aattgatgaa
    25921 agatgtcagc atacaggctg atgtaacaga aatagggggt gagaaagatc tggagaaagg
    25981 gttgcaaaaa gcgattgatg ccagggattc tgttggtggt attgtggaat gcagagtaaa
    26041 aggattacct gttggattgg gtgaacctta ttttgattca ctggagtcag tattatcaca
    26101 ttttcttttt gctattcccg cagcaaaagg agttgaattc ggaagtggtt ttgctgcagc
    26161 aagaatgttt ggtagcctgc ataatgatgc cattgaaaac aaagaaggta aaaccaggac
    26221 caatcatgcc ggggggatcg tgggtggtat cagtaatggg aatgagctgg ttttccggtt
    26281 ggcagttaaa cccacatcat ccacaccgaa agaacaaaac agtttgaatt gggataccgg
    26341 taaaactgaa aatttttcca ttaaaggacg tcatgattta tgtgtagcct taagggcacc
    26401 ggttattgta gaagcagtag ccgctattgt aattgctgat tttatgttat tggaacaaaa
    26461 aataaaaaga gtattgtaat gcctgttatt tatcatgtta ctaaagctgc agattggaat
    26521 gtcgcaaaga aaaacggttt ttatgagcac ccctcattaa atgatgaagg ctttatacac
    26581 tgctcacagg cgaaccaggt agccggagtg ctggaaaggt attttgcagg acaaacagat
    26641 ttggtaaaac tggtgattga tactgataaa cttaccagta aatttgtttt tgactggtcg
    26701 ccatctactc aagatacatt tcctcatgtt tacggaccga ttaacgtgga tgcaataatt
    26761 gatgttatcg ctttataatt actcagcctc ttttgtttac aggtatcgta tttatccgat
    26821 ttgttcggca gctgaaacca ggttcagtta tattcgggta attgagcaga aatattgagg
    26881 aaatctacag gtcaaactaa attatattga tttgatttct tattttgtaa cctagagtta
    26941 gggttatata aaaattatta taaatacccg atgaatttct ggaagccacc acaccacaat
    27001 atgatgaaaa gaatagcata cccgttcatc agcagtttta ttttctattt catcgctgca
    27061 tcctgtaatg ggaaaaccgg taaaggacag gatacaattg caggttcacc ggaagagttg
    27121 cagcaaaaaa caacggaact gatacggagc gctgcagaga gtgcttttgc caaaaaagga
    27181 atactggctg attcggcaat tacaataggt cagccaaacc ttacccggat gctatatgca
    27241 aaaaatgaat atacccccat ttggtctgct aaacaaacat ggttgccctt tggagattca
    27301 ttatttgact ttatagaaca gtcacaactc tatgggttat ttcccgaaga ttatcatgca
    27361 attcaattga aagagatcag gacaaaattt tatattgata gtcttacaaa aaataacaaa
    27421 aaggatgctg ctttatgggc aaaagctgat atcatgctta ctgatgcatt tgttcggatc
    27481 gtaaaggatt taaagttagg acgtcttccc aatgatagtg taacactcag aaaggattct
    27541 gtactcagtg atgagtttta tctgcaacaa ttaaatgagt tacaaaaatc gggggtaacc
    27601 cgggtagttc agtcactgga acccgatcaa agagggtatc gtttattgaa agaaggtatt
    27661 aaagcatttc ttgctactgc cagttatcgt cagtttacaa aagtgccata tcctgaaaag
    27721 gacaagcaaa aattcaacag gttattgcaa aaacggttgt acgaagaagg atttttatca
    27781 ttcgatagta taactgctga ttcagcccaa ctggctgctg ccgtaaaatt gtttcaaaac
    27841 aaaaagggta ttactgttga tggcagggca ggtgagggaa caatcagaat gctgaatata
    27901 aatgatagag agagatttat ccggattgcc attagtatgg ataaatataa attacttccg
    27961 gaaaggatgc cctcaaaata tatatgggta aatgctgctg gcaacctcct ggagttgata
    28021 gatggggata aagtaaaaat aacttcccgg gttatttgcg gcaagccaaa aacaagaact
    28081 cctgttcttt caagtgttat ctcttcgctt atcacctatc ctcaatgggt tcctcctcca
    28141 agtattgttg gtaaagaaat attaccggca gtgaaaaaga atcctggtta cctggcacgg
    28201 aaaggattca gtcttttaga caaaaatgga gatgaggtgg atccatatac cgttgactgg
    28261 tccagatacg ggaataacat tccgtaccgc attgttcagg gaagtggtga tgccaatgca
    28321 ttgggtataa tgaagtttgt ttttgataac aaatactcag tatacctgca tgatactaac
    28381 cagcgttatt tattcgctaa tgcgatgcgt tcattgagtc atggttgtgt gcgggtgcag
    28441 gagtgggaaa agctggcttt ttacatcctg caaaatgaaa gtatggatgc tggcagcggg
    28501 gcagcaaaaa ttgattcagt aaaaacttgg ctgaagtata aacagaaaag aagtattccg
    28561 atcaggaata aaataccggt ctacatacgt tatataactt gtgcagggca aaacgggaat
    28621 atcgtgtttt tcgacgatgt ttatggtgaa gataaaatgc ttagggtgag attttttgca
    28681 ggtaaatgat ataaaaaaag cctttattat gcctttgtac aaatgaaaac agtctttttt
    28741 gaaaaaaaag tcttccaaac ccgtgaatat taagtaaata atttatttta ggatattgaa
    28801 catacctgtt atttttgccc agatttaaac caaactatct ctttatgaag aagataatat
    28861 ctgctttcat tgcgctgtac ctgcttgtac ctgctgccag ggcccaggat gacgaaataa
    28921 gaccaaaagc tcttggcgtc agtttttttc tgaacgattt cgtttctcct gaaagaatac
    28981 gtactacttc attgagccag gttctcagtg ataagagtat ggcaaaaatt aaggaaatgt
    29041 caccgggtct tgctgttact tattttaaag gtatcagaaa gcatatcgat atcgctgctt
    29101 ctcttggggc tgcatttctt cgttacccga tgcccggaaa atcttttgca gacaataatt
    29161 tacttcttga gggtaatgct accattaacc tgaagatgac aactgagaaa tattgggtac
    29221 aaccttacct gatcgcaggc gtaggtgctc ataaatatcg atcttattat ggagctttcc
    29281 tgccagtggg ccttggtctt aaggtaaact ttcttgatga ggcacacctt tttgtaactt
    29341 ctacttacag ggtacctgtt accaacgaaa cagcaaacta tcatttacaa catagtattg
    29401 gtattgcagg tcggttaggt aagaagaaag aaccagtagt tattgaacct ccaaagccac
    29461 ccgcagatac tgatggtgac ggaattattg atagccttga taaatgcccg actgtaaaag
    29521 gattggcaaa gtatgatggt tgcccgattc cagataccga taaagacggc atcaatgatg
    29581 aagaagataa atgtccgact gttcccggtc ttgcccgtta ccagggatgc ccgattcctg
    29641 ataccgataa agatggcata aacgatgaag aggataagtg tcccactgtg gcaggcgttg
    29701 cccgttacca gggatgccca ataccggata cagatggtga tggagttaat gatgaagaag
    29761 ataaatgtgt gacaattccc gggccaaggg agaattttgg ttgtcctgtt atatctgaag
    29821 aagttaaaaa gaaagtgaat atggcagcta ataatattct ctttgttacg ggtagtgcca
    29881 aattgcaaac aaaatctttc aagggattga atgaggttgc tcagattttg aaagataatc
    29941 ctggtatgag ccttaatatt gacgggcata cagataatgt aggtagtgat gaaaaaaacc
    30001 aggtattaag tgataacagg gcaaattcag taaaggcata tcttgttagc aaaggggtgg
    30061 aggaaagccg tattgctgca cagggatatg gggagacaaa accaattgca gataataaaa
    30121 cagctgcagg tcgacagaaa aaccgccgct ctgaattgac attgagttat tataaataag
    30181 tgaatcctat acaaaaatga aaaatgtcct cttacaaggg gacatttttc attttatcca
    30241 cagtggttac taagtgggca ttatgacttt gtattccatg tttttccctt acctttgcgc
    30301 ctccaaaacc gtatggtttt ttaatagtat ctaaataact gaaaataaag cagtcatggc
    30361 agatttaaaa tttcaaagaa actttggtat tgccgctcat attgatgccg gtaaaaccac
    30421 gacaacggag cgtatcctcc gttacactgg tatgatccac cgtattgggg aagtgcatga
    30481 tggcgctgcc actactgact ggatggaaca ggaaaaagaa agaggtatta ctattacatc
    30541 tgcagctgtt agttgccagt ggaattttcc tacagtattg ggaaaagcaa ctcccgaaac
    30601 caaaaaatat tctttcaata ttatcgatac tcccggtcac gttgacttta cggttgaggt
    30661 tgagcgttca atgagggttc tggatggttt gattgctcta ttttctgcag ttgacggtgt
    30721 ggaaccacag tctgaaacag tatggcgtca ggctaaccgc tatggcgttc cccgtatcgg
    30781 tttcgtaaac aagatggatc gtgccggtgc tgacttccta atggtagtaa agcaggtaaa
    30841 agaaatgctt ggttccaaag cagtgcccct tcagttgccg atcggcgctg aagatgattt
    30901 caaaggggtg gttgatctga ttaaaatgaa aggtattatc tggcacatgg aaacagaggg
    30961 aatgaccttt gatgaaatag aagtgcctgc agatatgtta gaagaagcaa aggagtggag
    31021 agccagttta gttgaagcgg ttgctgaata cgatgataag ttaatggaaa aattctttga
    31081 tgatcccaac agtatcaccg aagatgaaat gcatgaggcg atccgtaaag caacggttga
    31141 cctgagcatt gttccgatga tgtgtggttc ttcctttaaa aataaagggg tacaaactgc
    31201 tcttgatgcg gtttgccgtt atcttccatc accaattgat atccctgata caattggtac
    31261 aaatcctgat actggtgagc agatcagccg taaagccgat ccaaaagagc cttttgcagc
    31321 tttggctttc aagatcatga cagatccatt tgtagggcgt ctggccttct tccgttgtta
    31381 ttcaggacac ctggacgctg gttcttatgt attaaacgta agaagtggta agaaagaacg
    31441 gatcagccgt attatgaaaa tgtttgccaa taagcaaaac ccgatcgatt tcatcgaggc
    31501 tggcgatatt ggtgcagctg ttggtttcaa agagatcaaa acaggagata cactttgtga
    31561 cgaaaaccac ccgatcaccc tggaaaatat gttcatcccg gaaccggtta ttgcggtggc
    31621 agtcgaaccg aaaacccagg ctgacgttga taaaatgggg atggccattg caaagctggt
    31681 tgaagaagat cctacgttgc gtgtaaatac agacgaagat acaggtcaaa ccatccttcg
    31741 gggaatgggc gagttgcatc tggagatcat tattgatcgt atgcgccggg agtttaaagt
    31801 tgaagtaaac cagggtgcgc cgcaggttgc ttataaagaa gctttccagg ctactgttga
    31861 gcaccgggag acattgaaga aacagacagg tggtcgtggt aaatttgccg acattcagtt
    31921 tagcctcggt ccgatagatg cagaatggaa aaccgagaat cctgacaaaa actatcaatt
    31981 cgttaacgat ctgtttggtg ggtcaattcc ccgtgaattt gttccggcca ttcaaaaggg
    32041 ttttgagcaa tccatgacta ccggtgtatt agctaactat ccagtcgaca atatgaagat
    32101 ccgtgtattt gatggtagtt tccatgctgt tgactcagac tctatgtctt tcgaactttg
    32161 cgccaagcaa ggtttccgtg aagcagcgag aaaggcaaaa cctgttttat tggaaccaat
    32221 catgagagta gaagtgatta ctcctgctca atacatggga gatgtaacgg gtgaccttaa
    32281 ccgccgacgt ggcatgctcg aaggtatgga tacccgtgcc ggtgcagaag ttattaaagc
    32341 taaagtgcca ttgagtgaaa tgtttggata tgtaacccaa ctccgttcat tgagctcagg
    32401 aagagcttcc tctaccatgg aattttctca ctattctccg gcaccaacta acattgctga
    32461 agaagtgata gcgaaggtga aaggtaaagt gagtgcttaa tattaaatag taaatgaaat
    32521 gaatactact cgaccccggc taaagccggg gttttttatt agaaattgaa aatattgttt
    32581 tgaatcaatc ctattttagt tctatggcac tgtgtttgtt atgaaatata ggaatttccc
    32641 cgtttaggat tcaagttcct gttaaaggcc tgatttcaag attatgaaaa acacaagttc
    32701 aagagattaa gggttcatca ctgaaccctt tttcttttag agggctcttg aaaacataat
    32761 acatcccgca tgcagaagtg aacaaagtga tcaggtagaa tagtatactt attacaacag
    32821 aagtttcctg cgataatcca aagtactcag acccctccag aaacacaacc tctctgattc
    32881 ccaatccacc tatggttaat ggtaaaacag atacaattga agaaattaga aaaataaaaa
    32941 tgtattctgt ggaatgggca ggtattttta aagcggccat tattaaatag acacaaacaa
    33001 cctgggaggc ttgtacaata atgcctaata gtaatgtagg ggcaaaactg gaaataaaat
    33061 ctttaaagta tcttttaatg ataaagtagg atgcctgcac tgcaagtaaa gaacctatta
    33121 ccagaaatgt gatatacaaa ttattgtcaa gcgtaattat tgagtaagtt gccaatacca
    33181 aacccaggcc cagtaagcca ctgaaacgat caagcaaaac cgcagcagta gttttttgat
    33241 aaggaacatt atacttttta gtcagaataa tcactttata ggcatcacca ctaatggatc
    33301 cgggaagaaa taaattataa aacataccca gccagtatag ttttatattc tcccattccg
    33361 gcaagtggac gttgatattt ttaaaataga tattcagtct tttggcagaa atgattttgg
    33421 aaaaaataaa tagaaacagg gccgcaaata aataaaacca actggtattt tttaatgcat
    33481 cgcctgcttt tgcaaaatca atttttccgg aaacatacca aaggcaaacc actgtgatga
    33541 ttacctttag tagcagttta agtaccttat tggttttaga ttgggtattc tttgatttgc
    33601 ccattattgc agtaaattgc tgcaagtaaa ttcaaattga tgataaaaag aattcttcct
    33661 tttattctgt ttgttttttg tgctggtatg tgttatgggc aggagaagcc tcttgtagca
    33721 ggtatgaaga tactccggac aatgaaaatc aaaaaagcgg tttaccagat agaagctccg
    33781 gctgatactg caaaacctgt actttttata gaaggagata atattactat tgattttaat
    33841 aatgccgaaa tgaatggcag taacaggaat aagaatcctg aagaattttc cggcgtagcc
    33901 atactcattc gcaatagtaa gaatgttaca atcaggaatt taaaagcaag aggatataaa
    33961 atagcattac tggcaagaaa tgtagaaaaa cttacattgg ataattgtga tttcagttac
    34021 aactaccggc agcatctcaa cagtacacag gagaaagaag atgtatctga ctggatgagc
    34081 tatcaccata atgaaaatga tgaatggttg cgatatggag ctgcgatgta cctgcggggg
    34141 tgcaattttg tgactataaa gaattgtaaa gtaaccgggg gccaaaatgc actaatgttg
    34201 atggaatgta atgatgggat gatatacaac aatgatttct ccttcaactc tggtattggt
    34261 atcggtatgt accagagtaa caggaacagt ataatgtaca acagggtagt ctttaatgtt
    34321 cggggctata gtcatggagt gtacaacagg gggcaggata gtgcaggtat gttggtatat
    34381 gagcaaagca gtaataatat tttttataag aataatgtaa cacatggggg tgacggtttc
    34441 tttctatggg ccgggcaaac aacaatggat aatggaaagg gcgggtgtaa tgacaatatc
    34501 attatgagta atgatttttc ctatgcccct acgaatggaa ttgaagctac atttagccgg
    34561 aatacaatct tgtcaaacaa aatatttgaa tgtgaccatg gtatttgggg agggtacagt
    34621 tataatacaa ctatcgcaga aaaccagttt agaaataaca ggattgctat cgccattgaa
    34681 catggacaat acaataattt ccatcataat ctttttataa aagataaaga ggcggtgagg
    34741 ttatgggcca ggcctcagca acccgaaaac tgggggtatg ctaaatacag gaacaccagg
    34801 agtgttggtt atgtaatgtc atcaaacagt tttaatgatc accagctggt ttataatatc
    34861 acaagaacag attcgctgaa tatttttgat aatac
//
LOCUS       DAORTI010000021        33640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_855_length_33640_cov_8.738038, whole genome shotgun sequence.
ACCESSION   DAORTI010000021 DAORTI010000000
VERSION     DAORTI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 33640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33640
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_33640_cov_8.738038"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1568)
                     /locus_tag="PLO70_04785"
     CDS             complement(204..1568)
                     /locus_tag="PLO70_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ73804.1"
                     /translation="MQLNSSITIKEGKTLPSLSENSILRYFNFIALYFAQGVPEGMLF
                     YGIPAWMAMNGNTPGEIAGFAVACGLPWSFKFIVAPLMDRYTYLPMGRKRPWVILGQI
                     GLIASCIYMAYVPDPLNNLNQFMLAGFMVSFFGAFQDVATDGMAVDIIPANQQARANG
                     LMWGSKIIAISTTLALGTWLLNTYNFTTAILMLAFMIAVIMLVPLFLRERQGEKITPW
                     SPGAASPETKKLQLTSWVSIFKSLYSVFSLRNSLLLALILFICQGAYNYLDTLLPIFT
                     VKELHWSNGDYSQLYAPAKIIGGITGMLIGGILIDKFGKKRMMNIYFFVMILLTTALV
                     CLKMYWTSLTFIYGFMMLYNVAYTFACIGIFAIAMQCCWKKVSASQFTLYMTIANLGR
                     IALAALIGPINTNFNWQGSLFAFAIMIALAWLLLQFFNIKKQAEQVVVLENKDNGGGQ
                     LQAG"
     gene            complement(1696..>2307)
                     /locus_tag="PLO70_04790"
     CDS             complement(1696..>2307)
                     /locus_tag="PLO70_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73805.1"
                     /translation="EFEKALATHAQKYHTGDAHWRVFTIQSGPDYGGYHITEGPTSWA
                     GEDARGDLGAEHMQDWNKNIAVHLTERESGGYSVYQDSLSTVALGDFSDKINISHIYP
                     KVAQGDNVVKIIKKLKKTWEASGVTVAVYFASSSGPAQYTLVTRYKQGLKERAAGFRK
                     PFKEVYESVNGEGSHAQYLKDVAEYINESWSELLFLRKELSSK"
     assembly_gap    2308..2407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2533..6645
                     /locus_tag="PLO70_04795"
     CDS             2533..6645
                     /locus_tag="PLO70_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ73806.1"
                     /translation="MPKQLSHIKLLLTVLFTGVAAVASGQKEKDYESISIAQGLSQGM
                     IFDLLQDKEGFIWVATKNGLNRYDGYGFKVFTNDPYNANSLSSNTLIKLFEDSKDRLW
                     VGTEDAGINVYDKKGDRFFRMQHKDADATSLSGNTIRTIAELPDGRMLVAVYGAGFNI
                     IDMSNDFFEKPSAPGITRLRLPNNTDVYAMGKDKNGQMWIAGFDGSVYRFDPAKNTFL
                     ALNNGRLLNDGYLTNGGEVLSSTNYFLSDGTAVYPLFDTTKIPAGNILLRPKVVLWRN
                     HYRELFKYDVTKRIPGKPLQWDMQFPDSLKVLFPFIIDRSGIVWSGSMGYGLRKYNTA
                     GSKFNQHLLPGYSVRWIKADKANTIYTSDFGYNWIKINEQGIEKDPFLKYPQLSAVDN
                     FLISKNGKYWFRPEAEGYKGYFEYNPVSGSLTKLLEYNNEASGDKQPMLEDSKGYVWF
                     SVRGGLFRRIQSNTGSIDSFSITVNNAKQGSSNIVCTALYEDRQGIYWIGTQEGFAKV
                     AFQNTANSNPQVKWFYTNSATRNSLNYNHVSCFLDDPAEPDKYLWIATKGGGLNRLDK
                     TTGDFSHLTTKDGLPDDVVYGILTDAAGNIWGSTNKGIFCMLATQNKSGTAYRFRNFT
                     KTAGLQDDEFNTGAFAKLPNGNLAFGGINGLNIFNPSTVLQSGFSPNVFITNILVGNK
                     VVLPNDNTGVLQSAIEQTQSITLNHLQDILTLEFSSLDFTAPDQNKYRYQLVGIDDDW
                     VESGTRRSATYLHLPSGTYVFRVQGSNSQGVWSDKIAELKIRVNPPWWLTWWAFMVYA
                     LLLVWGVRSYFKFRINKAKLQSQLSFEQQEAKRIKELDAVKTQLYANITHEFRTPLTV
                     ILGMANQVKSNPEKHLHNGVDMIVRNGENLLNLVNEMLDLSKLEDGKMTLNLINGDII
                     TFLRYIVESFQSLAASQQKQFHFLADTDELVVAFDAEKIRQIITNLFSNALKFTPPLG
                     NVYVSISQEAGTSSDNTILVLKVKDTGIGIPENQIAHIFDRFYQLDNSHTRKAEGTGI
                     GLSLTRELVKLMNGTIAVKSPPVGATKGTEFIITIPLAKAHSADGENFMIQPLKENST
                     FIDTEEQPGYYNIDPVNTSSTNALVLLVEDNADVVAYTASCLPDYRLAVGKDGREGFE
                     IAVDKIPDLIITDVMMPFVDGFEMCRKLRQDVRTSHIPIIMLTAKADMQSKLEGIEKG
                     ADVYLEKPFHKEELLLRIKKLLELRKNLQQYYSKQIGIIDNNGSLLKQETETPVEEKA
                     EHEFVQKVRAIVEANFNNYEFSVEQLCKLVFMSHSQLHRKLEALTGCSPNKFIRIVRL
                     KKAKELLVNPSLSIAAIALDCGYNDPGYFARVFKQEIGLTPQDWRSRKSN"
     gene            complement(6874..8442)
                     /locus_tag="PLO70_04800"
     CDS             complement(6874..8442)
                     /locus_tag="PLO70_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73807.1"
                     /translation="MSPARLLPSCIIITLLFIAGCSSGKDKPVDHLGEISFTVTGKAE
                     AQPAFKKGLLLLHSFEYADAAEAFQQAKETDPDFVMAWWGEAMTKNHPLWQEQDYDEG
                     NRILNQLAPTPEARIAKAKTALEKDFIAGINILYGNGNKAERDSNYAVFMETLYKKYP
                     GDNEVASFYSLALNGWGTTEQQTSILEAAAKIGYEVLLRNPKHPGALHYIIHAYDNPD
                     FAALALATADKYAAVAPDAGHALHMPTHTYLALGLWDKVVSSNEVSWAAEQARMHRKK
                     LDNDALGYHAYHWLQYGYLQQGNAKKARVMVDSMRQYSNAKPSPRARAHMILLKTTYL
                     AETNDYKSGVADITVEQKDMNITSRARNYFVAGMNEYYKNNATALDDVIQQLAGERLV
                     EEAKAAEKGIRICGNINRSLATKTDLLEAEAMEKQLRALQAWLKKDAATTEKLFKEAT
                     ALHTSSGYSYGPPAIVKPSFEMYGEWLLENNRPKEALEQFELALKLMPNKRLSLQGKE
                     AALKQLGQKEIAAL"
     gene            complement(8690..10120)
                     /locus_tag="PLO70_04805"
     CDS             complement(8690..10120)
                     /locus_tag="PLO70_04805"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ73808.1"
                     /translation="MKMYNHSLSAIKKIFPLVLFFLSACSSKKETKNEFAGKADEFFS
                     GQMKYYRFNGNVLIAEKGNIIFQQSYGFADFDSGRMLNDSSVFELASVSKQFTAMGIL
                     LLMEKGKLTLTDSLRHYFPELPYSNITIRHMLTHTSGLPDYESAMNAKWDKTKIAFNK
                     DMIALLAKEKPPASFVPGTKWEYSNTAYAILASIIEKVSGQTFAAFLAENIFKPLGMQ
                     HSRIYNTRRSLKDTIANYAYGYIYKPETKKYILPDNDTTTNFVIYLDGIQGDGIVNST
                     TGDLLKWDRALKNHTLLSEATQKEMLRGQELADTINKTYYGYGVMIEKTEAGENITHS
                     GGWPGYTTNLSRNVDADRTIIILSNNSANAPSLSKTMEHLFAGKPVIPPYEHKEIIID
                     SSALKKFTGSYEGRAKIRLEQKGTKLFRITTTGQSLELKPESNTKFFYTDGSDRQIEF
                     ELDTINKVSKVWLIINGLKTELKKTE"
     gene            complement(10130..11671)
                     /locus_tag="PLO70_04810"
     CDS             complement(10130..11671)
                     /locus_tag="PLO70_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73809.1"
                     /translation="MKYRKTILLLTAVMVSMAFLSFSVSLYNNNEIPKLWDFKKLKSA
                     HLPLVDKSIDVEPIPEELYYKIPERNAYKMYPMYLPGREPKGYYEWLLQQEPEIVFDA
                     STIKTEEDWVKAGELIYDMPQNFDPFIDSAVNMQELEGMASFLNAMHVPVAKDGTIPF
                     FNLVIRKKGKIEAGSFSCGNCHNKVMPDGSVLKGGQGNYPFDDIFGAGLKGRAEETKM
                     PDSAANAMISQSFSGLFGAPWIKHESQEFIKNPEWRRATNVLMATVPGVMHRHSSAFG
                     APTAIPDLFNLKERKYLDRTGLILHRDIGDVMRYAVLNQEMDFTNSYGGFKPDTSPDS
                     IIAKRYTRFSDAQAFALAKYLYSLKSLPNPDKAPAALIAKGKAIFEQETCVDCHTPPL
                     YSSNKLTPASGFTPSKEDKINYDIIELSVKTDPDLAMYSRRGTGYYKVPSLIGVWNRS
                     ALMHSGYVTSLEEMFNPARLKDNFIPSGFNPNVDKPFAVKGHTFGLYLDEEDKKALLA
                     FVRSL"
     gene            complement(11728..12993)
                     /locus_tag="PLO70_04815"
     CDS             complement(11728..12993)
                     /locus_tag="PLO70_04815"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQZ73810.1"
                     /translation="MQALAPVKANEREIFMDVLRGFAILGIFIANLNGFSWYREIPRS
                     EAGPYLLPEADRVMSFLHHLFIEGKFYSIFSLLFGWGIALQFKRAAANGINALPTVRR
                     RLFFMLLLGALHLLIWPGDIVFFYALLSFILLPLRKFSNKTLLITGSILILSPILLYW
                     LKMTWPVLNYPAELMIKTGIKVDDALLSLKSEEEFMDMMKNGNWFDQLKSNISGFFFR
                     YQYLFFVSRVPKVLGMFLIGYVVGRSDFYKYILQHKKLLVQIIITGALIGLPANYFLA
                     TYMQHHMRDYFQLKTNGLYQTIAYALGVAPLALAYVGVFILVFQTKTGKKILSVFAPV
                     GKMAFSNYITQSLIGNFVFLGAGLGFMWQVGPVYYTIFGIAVFSAQVILSTLWLKYFN
                     YGPVEWLWRSATYKKWQPMRKRNTDSGES"
     gene            13152..13793
                     /locus_tag="PLO70_04820"
     CDS             13152..13793
                     /locus_tag="PLO70_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ73811.1"
                     /translation="MSLDIAEIKKYLPVFTEELMAEIANYGLIKEIPEGVEILKEGQY
                     IKAIPIVLEGLVKVFTRMEEKELLLYYIGPAQSCIMSFSAGMSNAPSRIFAFTEDASI
                     ILLLPSDKLGKWVKQYPAINELFFQQYNLRYTEMLDTINSLLFDKMDQRLYNYLLEKS
                     KLKGEKILTVRHKQIAGELGTAREVITRVMKRLEQEGKVRQLPEGIQIIQAGD"
     gene            13957..14166
                     /locus_tag="PLO70_04825"
     CDS             13957..14166
                     /locus_tag="PLO70_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQZ73812.1"
                     /translation="MKQNMGTADRIIRVLIAAVFAYLYFSGTVTGTLGLVLVIAGGVF
                     VLTSLVSFCPLYTLLGITTCPAKKQ"
     gene            complement(14221..16362)
                     /locus_tag="PLO70_04830"
     CDS             complement(14221..16362)
                     /locus_tag="PLO70_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73813.1"
                     /translation="MRKLFFLFLCSTFFYQQDSSAQIKAKNFWPITGDIAIKNFHLNY
                     RASEGDIIYQFFLSDCIEGNCKNGEGVWASTNSRKVEFRDNGSLELTVNLYKGTFSDD
                     GRKFNGKKLVYNYTLSGSGAKNRDFYANKFTPLAAENLSSDLNNSIAGSKYIVAEGEA
                     IRTETKIGDKTTYSYTLHGIGASREAMTELGAASMQGKYENGQLQMAKIMVQENFPAK
                     SFTGKMNFKNKPLVGKLEFKDGTWYEGFFYDGKYNGPGKLVDTDGKTIQGFWDNGILK
                     DSQEVNLPSSLWDYNNAAMAESKPLTITMGEKSYTGNYTGEWKDGKPHGNGLFSNNSN
                     TFFYSRFENGMANGLGYFYSLFKTETWACCLRQSEYVLSGLFTNNWLIYGNKSIYEYM
                     WYTSSSDPNYTKGYIPQNSYLFSGTFKNHSLDGCGKSVLKRLSSETELSCTMTGTFKD
                     GGLHGWATVDFPLKRPDDKWKGKYFGYFIYHPVSPYAVKQDLITSFYSSTLPSDPIKY
                     YSGYEKEVEIFLKEITAGESVCDNITISEADKIKYIQDAKELGLYARKLVTNYKNQPP
                     PTTLVSTYDNAVKQIKYKAGTTLRKKQDDYLTSVITGYDFTTQKYSVTNAIYQAVYRK
                     DGTFKTLKDASKTVSLTEQEIEKIYNVQSQLYSICSVCKGKGYTTGTVHGGNQGGWEK
                     WTDNVFYNRPGTAYSYEVTTGCGACKTVGWK"
     gene            complement(16429..16974)
                     /locus_tag="PLO70_04835"
     CDS             complement(16429..16974)
                     /locus_tag="PLO70_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73814.1"
                     /translation="MEQTTLGIGTRLQHTHFGPGVIVGIRYATYLISFINHGIKEIDK
                     TDNKLEEIIPENVSAEIETHSEVEMSLLKILRLWGGLTETVPLGDKWIKGILLLQPAD
                     KSLKPKEIPIEDFFHKIVMLRDRLRVLEQNINSSKTLNDEEKVNIQQYITRCYGSLTT
                     FNVLFKNKEHWFVGEKGSKED"
     gene            17110..20469
                     /locus_tag="PLO70_04840"
     CDS             17110..20469
                     /locus_tag="PLO70_04840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQZ73815.1"
                     /translation="MKRISFLLILTGFVVPVLSQVKQLPVQKKDEIKVVTTVSQPVGV
                     IAMRGFDFERGKDALADWTKEGTAFNNQPVYGHNVIIRREMDNTYNPFRINISLGGDY
                     WKDLSLPIGHKGNYWIGSYENRSDLTQPFGRTQGNIPTGVLISPEFTITKDYISFLIG
                     GTNDLANAKVELGYEGQGETDTEEKKEKDPFRVIAPVIKTIPVVKQFRATGKGSSVMR
                     RVWWDVKTLKGKKARIKITDNTSTGYINADDFIFQDAAPVDTDIAINSKVNVKQVFQK
                     EGNYYDWDFPLWGTIDTHTHPASHIGFGNRLFHGEPVGTINKALADCEIGHGADIFRT
                     LTSSSGERISGNIIRNAVAGAFDPGHRLGVMDPGNTWGSNEQTLAYWPLYNTKLHQQM
                     WKDWLKRAFDGGVRMIVALTVHSQVFARISEGTRPDDDKTVGDKQIDYIKNVIAPACR
                     DWMEIAYSPADMRRIMLSNKLAVVLGSELDNIGNFDGKRTPTEAEIRAEIRRLYNKGL
                     RYMFPVHLLNNGLGGTAIKSDIFNLATKFNTGRPFDVELKNYKDGFSEYKLKAKFDVL
                     SDMPAAIIDANNKVAAYAGIAGAAAPLVLPAVAPAILPFIPLITTVGTSVVGIAAPLI
                     PILATVAINKWTNEWGISMDVIPVGNNYPAYPWKRYAGHINKQGLTTAGEIAVNEMMK
                     LGMMIDIDHAGRKTVDDYFEKAFAVPGGGYPLNSGHNQFTETRWTEAQLLPEEYDLRS
                     EEGRKLYTNHYNHIAEKIANENDRKPDELRKIKQLGGIMGVGISGANVNAISNIIPAS
                     GARYIESVVKNDNPGSSKSAAQLLLYAIEKMEGSGVCFGTDINGFAGLVGPRFGANSA
                     SAAGHDRFDIYNRQSFVYSQANQVKYRSFTDLTGNRFRGHLTGSYDELKSSPDRFLQN
                     GKLFTDEQGDFMLALQIYYSMEERLKKVAESGDTKNKIDWYLNNVVHNSYNKGRIREF
                     IKGLIKGGNEEGIGTDVFNGDVGTQQQIACKVSREKIFNKRTFDNRVADVAINAEKYK
                     RHKLLEQALNYYKIMMKPGTNEPLIPSSTLGKTWDYNLDGMAHYGMMADFFQDLKNVG
                     MKPKDMSVLFNSAEDFARMWERCLRQSERVR"
     gene            complement(20540..21301)
                     /locus_tag="PLO70_04845"
     CDS             complement(20540..21301)
                     /locus_tag="PLO70_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73816.1"
                     /translation="MKHPIYILFIISFLFTATSVIAQTYKIKGVVYDSSRNYPMELVS
                     VLSTSGTGTVTNADGYYEIEVTEKDSIWFSYLNKPTVKFPVMRIGNPMQFDISLQVNV
                     PVMKEVKILPRNYRLDSLRNRQDYAKIFNYQKPTLKTVTPQYGAAVGFDLDEIINMFR
                     VKRNRSMASFQRRLLMEEQDKYVDFRFNKALVRRLTLLDGAELDSFMRVFKPSYTFTK
                     LAGDYEFRLYIKQALYRFKRGMQPDAWYKEELEEQ"
     gene            complement(21421..21996)
                     /gene="purN"
                     /locus_tag="PLO70_04850"
     CDS             complement(21421..21996)
                     /gene="purN"
                     /locus_tag="PLO70_04850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQZ73817.1"
                     /translation="MNKIAVFASGGGSNAQKIIEYFNNSQLASIALIVSNKPEAGVVT
                     IAEKNTIPTLIIEKEKFLIGSGYADDLKKEGIDLIVLAGFLWKIPALLIKTFPGKIIN
                     IHPALLPKYGGKGMYGFHVHQAVIDNKEKESGITIHYVDEQYDHGQPIFQATCPVFEN
                     DTAFSLAKRVQQLEHEHYPKVIEEILFNNSK"
     gene            complement(22036..22326)
                     /locus_tag="PLO70_04855"
     CDS             complement(22036..22326)
                     /locus_tag="PLO70_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73818.1"
                     /translation="MFERLQKKWKVNGLQLALILCTFTIGGSATGFVGKKIMNALSIG
                     QDWLWAVIYILLVTLIWPLMVIIVSIPFGQFRFFNAYIHKLGQKMGVIREKK"
     gene            22597..23868
                     /locus_tag="PLO70_04860"
     CDS             22597..23868
                     /locus_tag="PLO70_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQZ73819.1"
                     /translation="MILCKPIGQRLTSLFLSFFVFSIVSVTAQDIAAGKAIFNSKCAA
                     CHNVLKDGTGPALAGIEERHKWADHKELLAWINNPASYMAKDPYTQGLKAKYGSMMTG
                     FADVTLADVGNLVSYINEAAKPAPPPPPGAEVQPSGNQNAIIFGVISLILGIIALILM
                     QVNSNLKKMSDDSEGIIRPEPVPFYRNKVYIAMAAIIFFIFGGYMVAKGAIGLGRQKD
                     YQPEQPIYYSHKVHAGINQISCLYCHGSAWDSKHAAIPSVNTCMNCHKAITTYEKGPK
                     LYDEAGNEINGTAEIAKLHAAAGFDPNNANKWDPSMIKKPIEWVKIHNLPDHVYFNHS
                     QHIRAGNVQCQSCHGEITAMDEVKQVAELSMGWCINCHRQTKVDFNYDSTKGNKYYSI
                     YEKFHNDFKSGKMDSITVKDIGGLECQKCHY"
     gene            23940..27149
                     /locus_tag="PLO70_04865"
     CDS             23940..27149
                     /locus_tag="PLO70_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HQZ73820.1"
                     /translation="MSQKKYWQSFGEVNDNENFQKKQQDEFKEELPFEGFDDKGLIDA
                     KAPRRDFLKYLGFSTAAATLAASCKVPVKKAIPYANKPENLVPGVAKYYATTYVQDGD
                     VVPVVAKVRDGRPIKIEGNDLCSYTNGGTTARTQASVLDLYDTHRLRFPQHRVGKGAD
                     AKFEEVPTFEQFDKMVGDAIKNAGGTTVLLTSTIVSPSTKEIIANYPGLRHVQYDAVS
                     YSGMLLANEASGFGKKIPTYDFGAAKVIVSLGADFLGTWLNSVENAKGYSVGRRIDEK
                     NPSMSKHYQFEGYLSLTGASADERFTHRPSETGLVALGLAVAVGAVDMASPVTDAKLK
                     AGIEKVAADLKANAGAGLVVCGSNDKNIQVIVNAINNTIGAYGKTIDWSKPVNYRQGI
                     DSDFAQLVADMEAGTIGTILICGANPAYNYHDAAKFSAALSKVKVTVSFNERLDETTE
                     LCEYVAPSHHYLESWGDAEPKTGVVSFMQPTIYPLFKTRPFQTSLLKWSGNNTDYEVY
                     FKNYWTGKLGSEEAFNKILQDGIKEEAPAAAGAGTYNGGAVATAISAIESAKKVTGAE
                     VVLYQKTSIGTGTGATNPWLQELPDAISKATWDNYALISMSMAKEVLGLDLINGSEKE
                     SNNYEYYPQKPVIKLTVGKKEVELPVLVIPGMQANTIAVAVGYGRGEKLGKTAAGIGK
                     NVYPFTSFDGTTISYHAAVTVADAKRKEKIAQTQIHNSYEDRIEVVRETTLATFKKNP
                     NVIPDYRNHLEETYGKSQNGATTDYRKEGTLYPLHDQPGIKWGMNIDMNACYGCGACV
                     VACHAENNVPVVGKSEVLRYHDMHWLRIDRYFVSDDKNPDELKGVVFQPMMCQHCDNA
                     PCENVCPVAATNHSAEGINQMTYNRCIGTRYCANNCPFKVRRFNWADYTGADSFKDNQ
                     DQQLVGKLDPVVHQMNDDLTRMVLNPDVTVRSRGVIEKCSFCFQRLQAAKLEAKKQDR
                     PLKDGDAKTACQTACSANAIVFGNVHDKESAITKVRAENTQRGFYVLEQLHVLPNVSY
                     LAKVRNTEEIVESEGHHPPPASEHGAEGQGEKKPAEAQH"
     gene            27221..28675
                     /gene="nrfD"
                     /locus_tag="PLO70_04870"
     CDS             27221..28675
                     /gene="nrfD"
                     /locus_tag="PLO70_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQZ73821.1"
                     /translation="MALLRYESQVRAPLVDGTKDYHQVTEDICRPVEATPSKLWWIGF
                     LISVALLIFGIISVTMEVIYGTGQWNLNKTVGWGWDITNFVWWVGIGHAGTLISAILL
                     LFRQGWRTGVNRAAEAMTIFAVMCAGQFPIWHMGRVWNAFFVLPYPNTRGPLWVNFNS
                     PLLWDVFAISTYFTVSLLFWYSGLLPDLATLRDRAKVKWRKAFYGVAAFGWTGSTKHW
                     QRHEALSLVLAGLSTPLVLSVHTIVSFDFATSVVPGWHTTIFPPYFVAGAIFSGFAMV
                     QTLLVVTRKVLALEDYITLEHIEVMNKVIVLTGSIVGIAYLTELFMAWYSAVGYEEFA
                     FFENRLNLNSPYGWSYWIMMGCNVLSPQIFWFSKMRRNLVVTFFMSILINIGMWFERF
                     VIIVTSVYRDYLPSAWSTYYTPTIWEVGFYLGTFGLFFTCYFLFSKFFPVIALAEIKH
                     ILKKNGEHYKDHMDEIEEESTEEFGQKHGHDHGA"
     gene            28780..29370
                     /locus_tag="PLO70_04875"
     CDS             28780..29370
                     /locus_tag="PLO70_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HQZ73822.1"
                     /translation="MAVKKFVVGNFDDEAVLFPAVKKVRRAGYKIHDVFTPFPIHGLD
                     KVMGLRDTSLHTAGFIYGITGTATAVGFITWALTVDWQINFGGKPFFALPAWIPVTFE
                     LTVLFAAVGMVLTFCYLCQLAPFVKKDHFNPRSTDDIFVMALECTDKTNEAEAMSFLE
                     TIGAKEVKVEYKETEWWLGRYDKDRMRFQKKEQDNH"
     gene            29389..30021
                     /locus_tag="PLO70_04880"
     CDS             29389..30021
                     /locus_tag="PLO70_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ73823.1"
                     /translation="MKKIFIVAGCLVLAVAMISCNNIRRDEGRAYMPDMAYSRAYETY
                     ASTEALDKAGVNYVSKPVDGTIARGEMAAYPFKNDSIGYVRSAEVKNPLDITAIDMKE
                     AERLYLVNCGICHGAKLDGNGPLWNGGNGAFPNAPRNLLTDPLVANMAEGTMFHSVTY
                     GRNNMGGYASQMSTKQRWEVVAYIKAKQGQGSTVVAPAAAPSDSTAAVKK"
     gene            30055..31284
                     /locus_tag="PLO70_04885"
     CDS             30055..31284
                     /locus_tag="PLO70_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HQZ73824.1"
                     /translation="MSIRENFVVPKRYNTIAFALMAIGILAIIGLYVTSGSKSDPKEQ
                     ARFWASLLQNSVFFLLVVNASMFFICATTLAWGGWQMAFRRVPEAISAVVPVIGVICG
                     AILLAIVFGGNHTIYHWTDTHHVAEDAILNHKKGFLNKGFFAVATVLTIVLWSVLGWK
                     MRQLSRSLDSKPLATVEARKKYIWNNTVWAALFIIVFSLTVMSSIPWLWLMSIDAHWY
                     STMYSWYTFASTFVSGMALIALFVVFLKNNNYLEYTNNEHLHDIGKFMFAFSVFWTYL
                     WFSQFMLIWYANIPEETIYFQPRAHGIYKGIFWLMFIINFIAPLLLLMTRDAKRNYGT
                     ITFMSLLIIFGHWLDFYQMVFPAVSPNKVPMILYDFGVALGFVGLIMFVTGRALSKAP
                     LLAKNHPFVKESLIHHT"
     gene            31337..32374
                     /locus_tag="PLO70_04890"
     CDS             31337..32374
                     /locus_tag="PLO70_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQZ73825.1"
                     /translation="MQTFFIVAILALGFLITFQIAKASEYVAVLRGYEKARKQTNKIN
                     GFMLLAFLIIGLIGVYYCNEKLKGKILGEPASDHGVLVDRMLYVTLLITFIVFVITQV
                     ALFWFSYKYQESDKRKAFYFAHSNKLELIWTVIPAITLTILVGFGIFYWFKITGDAPK
                     NAMQVEVVGKQFGWEFRYPGRDKILGKKYFKNVNPAANNPVGQIWDDPANHDDIFVEQ
                     EMHLVVNKPVKLVIGAKDVIHDVGLAHFRMKMDAVPGTPTTMWFTPTYTTKQMKEKTG
                     NPDFVYELSCDQMCGKGHWSMRGTIVVETQEEFDAWLASKKPQYLVANPDKDPSAPKA
                     DTVKQVAAVIK"
     gene            32461..>33640
                     /locus_tag="PLO70_04895"
     CDS             32461..>33640
                     /locus_tag="PLO70_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQZ73826.1"
                     /translation="MSEAMNVHSGVDAAHAVHHDDHGHGHHEQSFISKYIFSMDHKTI
                     GKQFLITGMVWAIIGGLFSVLFRLQLGYPDQSFPILETFFGNWAKGGQIQPEFYYGLV
                     TMHGTVLVFFVLTAGLSGTFANLLIPLQIGARDMASPFLNMLSYWFFFIAGIVMLASI
                     FVHTGPASGGWTMYPPLSALRQAGDGQKIGTDLWLISMALFIVSSLLGGLNYISTILN
                     MRTKGMSMTRLPLPIWAMFFTAVLGVLSFPVLLSGAILLLFDRNLGTSFYLSDIVING
                     EILPNEGGSAILYQHLFWFLGHPEVYIILLPAMGMVSEILSVNARKPIFGYMAMLGSL
                     FAIAILAFLVWAHHMFVTGLNPFLGSVFVLLTLLIAVPSAIKVFNWLTTLWRGNIRFT
                     P"
BASE COUNT         9805 a         6616 c         7334 g         9785 t
ORIGIN      
        1 cgcagaacac agcgaggaac aacattaaaa actcccagaa ataatgggtc catttcttcc
       61 ttgccgtatg tgagtggtgg tgtacttcca tgctgctggt ttatgttttt gtaataaaag
      121 aaagtctata aagacggcta gtaaccagca aaagataaga tttttattac ggggaatgta
      181 ccagcttttc ccttgaacgc ctgctaaccg gcctgcaact gtccgccccc gttatcttta
      241 ttttccagca ctactacctg ttctgcctgc tttttaatat taaaaaactg taaaagcaac
      301 caggccaatg caatcataat ggcaaatgca aacaacgaac cctgccagtt aaaatttgta
      361 ttaataggcc cgattaatgc agccagtgca atccggccaa gatttgcaat agtcatataa
      421 agcgtaaact ggcttgccga tacctttttc cagcagcatt gcatggcaat tgcaaaaatg
      481 ccaatgcagg caaatgtata agccacattg taaagcatca taaagccata aataaatgta
      541 aggctggtcc aatacatttt taaacagacc aatgcagtcg ttaaaaggat cattacaaaa
      601 aagtaaatat tcatcatccg ctttttccca aacttgtcaa ttaatatacc accgataagc
      661 atgccggtaa tgccacctat tatttttgcc ggggcgtaca attgggaata gtcaccattg
      721 gaccaatgca gttcttttac agtaaaaatg ggcagcaatg tatccaggta attgtaggct
      781 ccctggcaaa taaataatat taaagcaagc agcagcgagt tgcgtaaact aaatacactg
      841 tacaaggatt tgaaaataga aacccagctg gtgagttgta gttttttagt ttccggcgaa
      901 gctgcacccg gagaccaggg tgttattttt tcaccctgcc gttctcttaa aaacaaaggc
      961 acaagcataa taacagcaat cataaaagcc agcatgagga tggctgttgt aaaattgtat
     1021 gtattcagca gccaggttcc caatgcaagg gttgtagaaa tggcaataat cttggaaccc
     1081 cacatcagac cattggccct tgcctgttga ttggcaggta tgatatcaac cgccatccca
     1141 tcggtggcca catcttggaa agcaccaaaa aaggaaacca taaaaccagc cagcataaat
     1201 tggttcaggt tgtttaaagg gtcgggtaca taagccatgt aaatacaact ggcaatcagt
     1261 cctatttgcc ctaaaattac ccatggtctt ttgcgaccca tcggcaaata agtatagcgg
     1321 tccattagcg gcgctacaat aaatttaaaa ctccaaggca gcccgcaggc tactgcaaaa
     1381 ccggcaattt ctcccggcgt attcccattc atggccatcc acgccggtat accataaaat
     1441 aacatccctt ctggcacacc ctgtgcaaaa tacagggcaa taaaattgaa ataccgaaga
     1501 attgaatttt ccgaaagtga gggcagggtt ttgccctctt ttatagttat ggaactgttg
     1561 agttgcaagg tttacctttc ttgaatttta ttaaaatggg agaagtgttt atcagcactc
     1621 ctccctttta tatgattatg gtttaatggg taatggcctg cctattcatt cctgaatagt
     1681 ccgccccggt aaaacttatt ttgaacttaa ctccttacgt aagaaaagca actcactcca
     1741 gctttcatta atatattcag caacatcttt taagtattgt gcatgggagc cttctccgtt
     1801 aacggattca tatacttctt tgaatggctt gcgaaaacct gctgctcttt cttttagccc
     1861 ttgcttgtac cttgttacta aagtgtactg cgccggcccg gaagaactgg caaaatatac
     1921 tgccactgtt acaccggatg cctcccaagt ttttttcagc tttttgatga tttttaccac
     1981 attatccccc tgggcaactt tgggataaat atgggagata ttgattttgt cagaaaaatc
     2041 gcctaaggca accgtactta acgaatcctg gtaaacggag taaccgccgg actccctttc
     2101 ggtcaaatga accgcgatat ttttattcca atcctgcata tgctctgcgc ccagatcacc
     2161 tcttgcatct tcgccagccc agctggttgg accttcggta atatgataac cgccgtaatc
     2221 agggcctgac tgaatggtaa ataccctcca atgggcatcc ccggtatggt atttttgggc
     2281 atgggtggcc aatgcttttt caaactcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnttt tcagcaattg acaggacaaa ctgtgcagta acaggataaa ttcatcattt
     2461 tcgaaaaaac agtcttgatt tatttactcc ttgcaaacta taaaagcctg ttactgctat
     2521 ctttatagca gggtgccaaa acaacttagc catattaaat tgctgcttac cgttcttttt
     2581 actggggttg cagcagtggc ttctggtcaa aaagaaaaag actatgaaag tatttccata
     2641 gcgcagggct tatcgcaggg aatgattttc gatcttttgc aggacaaaga aggctttatc
     2701 tgggttgcta ccaaaaacgg cctgaaccgt tatgatggct atgggtttaa agtttttacg
     2761 aatgaccctt ataatgcaaa cagcctcagc agtaataccc ttattaaact atttgaagat
     2821 tctaaagaca ggctatgggt aggaacagaa gatgcaggca taaatgtata cgataaaaaa
     2881 ggcgatcggt tttttcgcat gcagcacaag gatgctgatg ctaccagcct ttccggaaat
     2941 acaattagga ctatcgcgga attgccggat ggcaggatgc tggtggctgt atatggcgcc
     3001 gggttcaata ttattgatat gtccaatgat ttttttgaaa aaccttccgc tccgggcata
     3061 accagattga ggcttccaaa taatacggat gtgtatgcca tgggtaaaga taagaatggt
     3121 caaatgtgga ttgctggttt tgatggatcc gtataccgat ttgatcctgc taaaaataca
     3181 ttccttgcat tgaataatgg ccgcctgctg aacgatgggt acttaaccaa tggaggggaa
     3241 gtgcttagta gtaccaatta ttttttatcg gatggcacag cagtatatcc gctgtttgat
     3301 acaacaaaga tacctgcagg taatatacta ctgcggccaa aggtagtttt gtggagaaac
     3361 cattaccgcg aattgtttaa gtatgatgta accaaacgca tacccggcaa accgcttcaa
     3421 tgggatatgc agtttccgga tagtttaaaa gtactattcc cttttatcat tgatcgctca
     3481 ggtattgtat ggtctggttc tatgggctat ggcttgcgta aatataatac ggctggcagt
     3541 aaattcaatc agcacctgct acccggttat agtgttcgat ggataaaagc agacaaagcg
     3601 aatactatat atacaagtga ttttggttac aactggataa aaataaatga acaggggatc
     3661 gaaaaagacc cttttcttaa ataccctcaa ctatccgctg ttgacaattt tttaattagt
     3721 aaaaatggaa aatattggtt caggccggag gcagaggggt acaaaggcta ttttgagtat
     3781 aatcctgttt cgggtagctt aacaaaactc ctggagtata acaatgaggc cagtggcgat
     3841 aaacaaccta tgctggaaga tagcaaaggg tatgtctggt tttcagtacg tggcggcctt
     3901 ttcagaagga tacagtctaa taccggcagc atagatagtt tttctataac tgtaaacaat
     3961 gcaaaacagg gatcttctaa tattgtttgt actgctcttt atgaagacag gcaaggcatt
     4021 tactggatag ggacgcagga aggatttgca aaagttgctt ttcaaaatac agccaatagt
     4081 aacccacagg tgaaatggtt ttataccaac agtgctaccc ggaattcact taactataat
     4141 catgtatcct gttttttgga cgaccctgcc gagcctgata aatatttatg gattgcaacc
     4201 aagggtggtg gcttaaacag gctcgataaa acaacaggtg atttttctca tctgactaca
     4261 aaggatggct taccggatga tgtagtgtat ggcatactaa cagatgctgc cggtaatatc
     4321 tggggcagca ccaataaagg tattttttgt atgttggcaa ctcaaaacaa gagtggaact
     4381 gcatatagat tcagaaactt taccaaaact gccggcttac aggatgatga atttaataca
     4441 ggggcttttg caaaactgcc caacgggaac cttgcttttg gtggtatcaa cgggttgaat
     4501 atttttaacc cgtcaacggt tttacagagt ggtttctccc ccaatgtttt tatcactaat
     4561 attctggtag gcaataaggt ggtgttgccc aatgataaca ccggtgtatt acaatctgcc
     4621 attgaacaaa cacagtctat tactttaaat catttgcagg atattttaac ccttgaattc
     4681 tcatcgcttg attttactgc acccgatcag aataaataca ggtatcaact ggttggaatt
     4741 gatgacgatt gggtggaaag cggtaccagg cgttcggcca cttacctgca tctgccatca
     4801 ggcacttacg tttttagggt tcagggcagc aacagccagg gggtatggag cgataaaata
     4861 gctgaattaa agatcagggt aaatcctccc tggtggctta cctggtgggc atttatggta
     4921 tatgctttat tgctggtatg gggagtcaga agttatttta aattcagaat aaataaagcc
     4981 aaactgcaat cgcaattaag ttttgagcaa caggaagcca aaagaataaa agagctggat
     5041 gcagtaaaga cgcaactcta cgccaacatt acccatgaat tcagaacacc gcttaccgtc
     5101 atattaggca tggctaacca ggttaaaagc aatcctgaaa agcacctgca caatggcgta
     5161 gatatgattg tgcgtaatgg tgagaattta ttgaacctgg taaatgaaat gctggatttg
     5221 tcgaaactgg aagatggtaa gatgacatta aacctgataa acggagatat cattactttc
     5281 ctgcgataca ttgtagaatc atttcagtct ttagccgcca gtcagcaaaa gcaatttcat
     5341 tttttggcag atacagatga attggtagtt gctttcgatg ctgaaaagat caggcagatc
     5401 attacgaatt tattttccaa tgccttgaaa tttacaccgc cattgggtaa tgtttatgtc
     5461 agtatttccc aggaggccgg taccagcagc gacaatacaa ttttagtatt aaaagtaaaa
     5521 gataccggta ttggtatacc tgaaaatcag atagctcaca tatttgaccg gttttatcaa
     5581 ttagacaaca gtcatacccg taaagcagaa ggaacaggca tcgggctttc actcaccagg
     5641 gaattagtaa aattaatgaa tggtaccatc gcggtaaaaa gcccgcccgt aggagctaca
     5701 aaaggaacgg agtttattat tacgatcccc ttagccaaag cccattctgc agatggagag
     5761 aattttatga tacaacccct gaaagaaaac agtacgttca tagacacaga agaacaaccc
     5821 ggctattata atattgaccc tgtaaatacc agcagtacca atgcattggt tttattggta
     5881 gaagataatg ctgatgtggt ggcctacaca gccagctgcc tgcctgatta caggttagcc
     5941 gtaggcaaag atggcaggga aggtttcgaa attgctgtag ataaaatacc cgatttaatt
     6001 attacagatg taatgatgcc ttttgtagat ggttttgaaa tgtgccgaaa gctgcgccag
     6061 gatgtacgga caagtcatat acctataata atgctgactg caaaagcaga tatgcaaagc
     6121 aaactggagg gtattgaaaa aggagctgat gtatatttag aaaagccatt tcataaagaa
     6181 gagttattgc tccgcataaa aaaattatta gagcttagaa agaacctgca acaatattac
     6241 agcaagcaaa ttggtattat tgataacaac ggatctttat taaaacagga aacggaaaca
     6301 cctgtagaag agaaagccga acatgaattt gtacaaaaag tcagagcaat agtggaggct
     6361 aattttaata attatgaatt tagtgtagag cagttatgta agttggtttt tatgagccac
     6421 tcgcaattgc accgtaaact ggaggcgctt accggctgct cacccaataa gtttatccgg
     6481 attgttcggt taaagaaggc aaaagaatta ttagtgaacc cgtctttaag tattgctgct
     6541 attgcccttg actgtggcta caatgacccc ggttattttg caagagtatt taaacaagag
     6601 ataggcttaa cgccacaaga ctggcggagc cgaaaatcaa attgataaaa aggtcctgtt
     6661 acttttttgc aataatggtc catttcttac tccggcgtaa aggagtggtg gtgtactttc
     6721 atttattgat cgtttttaaa atagcggtaa agaaaagcag ctgtaaatcc tatgagtaca
     6781 taaataataa ggcggatgat ccgccatttg gttgtactat tcataagcta taaagttgtt
     6841 ggttgaggtt ctccgctggt tatgattccg tttttataag gcagcgattt ctttttggcc
     6901 tagctgtttc aaagctgctt cttttccctg cagggataaa cgtttattgg gcatcagttt
     6961 taaagctagc tcaaattgtt ccagggcttc tttgggccgg ttattttcca gcagccattc
     7021 cccatacatt tcaaaagaag gttttacaat ggccggaggt ccgtaagaat aaccgctgct
     7081 ggtatgcagt gcagtagctt ctttaaataa tttttctgta gtcgctgcat cttttttcag
     7141 ccatgcctgc agggctctta attgcttttc cattgcttca gcttccagca ggtctgtttt
     7201 ggtagccagc gaccggttga tattaccaca aatgcggata cctttctctg cagcttttgc
     7261 ctcttcaaca agccgctctc ctgcgagctg ctgtatcaca tcatccaggg cagttgcatt
     7321 gtttttataa tattcattca tacctgcaac aaaataattc ctggcacggg atgtaatatt
     7381 catatctttt tgctccacgg taatatccgc cactccactt ttatagtcat tggtttctgc
     7441 caggtaagta gttttcaata agatcatgtg tgcccttgcc cggggactgg gcttagcatt
     7501 gctgtattga cgcatactat ccaccatcac ccttgctttt tttgcattgc cctgttgcag
     7561 gtaaccatat tgcagccagt gataagcatg ataacccagc gcatcattat ctaatttttt
     7621 ccgatgcatt cttgcctgct cggccgccca ggaaacttcg ttgctgctta ctaccttgtc
     7681 ccataaaccc aatgccaggt aggtatgggt aggcatatgc agggcatgac cggcatccgg
     7741 cgccactgca gcatatttat cagccgttgc taatgccagt gcagcaaaat caggattatc
     7801 ataggcatgt atgatatagt gcaatgcccc gggatgtttc ggatttcgca gtaatacttc
     7861 atacccgatt tttgcagcgg cttccagtat acttgtttgc tgctctgtag taccccatcc
     7921 gttcaacgcc aggctgtaaa aagaagccac ttcgttatcg cccgggtatt ttttatataa
     7981 tgtttccata aatacagcat aattgctgtc tctttctgct ttgttgccat taccatataa
     8041 tatattgatg ccggcgataa aatctttttc taatgctgtt ttggctttgg cgatccttgc
     8101 ttccggtgtt ggagccagtt gatttaagat cctgttgcct tcgtcataat cctgttcctg
     8161 ccagagcgga tgattctttg tcattgcttc gccccaccag gccataacaa aatccgggtc
     8221 tgtttctttg gcttgctgaa aggcctctgc tgcatctgca tattcaaaac tgtgcagcag
     8281 caacaatcct tttttaaaag ctggctgggc ttcggcttta ccggttacag taaaactgat
     8341 ctcaccgagg tgatcaacgg gtttatcctt accagacgaa caacctgcaa taaataataa
     8401 ggtaataata atacaggagg ggagaagtct ggcaggtgac atggtagttg gttttgtgtt
     8461 tttaaattta cttaattctt ccggatagta ttgcatattg ttttatgcaa tacaaggtct
     8521 gtttgcccaa tcacttaatc ttttaaatgc tacttgttaa tcagcgcctg tgagaacgcc
     8581 gcaaaaaaca cccgggaata ataggagaag atgggttcat ttcttgcggg tagtaggtga
     8641 ccctttagct atcgggtaat ggtatacttt cataccagta gttttaaagc tattccgttt
     8701 tctttaattc agtttttaat ccgtttatga tcaaccatac tttgctgact ttgttaatag
     8761 tatctaactc gaactcaatt tgccggtcag agccatccgt ataaaaaaac ttggtgtttg
     8821 actcaggttt aagttcaagt gattggccag ttgtagtaat acgaaaaagt tttgtccctt
     8881 tttgctcaag cctgattttt gcccttccct cataactgcc ggtaaatttt tttaatgcgg
     8941 atgagtctat aataatttct ttatgctcgt agggagggat aaccggtttg cctgcaaata
     9001 aatgttccat tgttttactg agcgacggag catttgcgct gttattggat aaaataataa
     9061 tcgtacggtc tgcgtccaca ttccgggaga gattggtagt atagcccggc cagccgccgg
     9121 aatgggtgat attttccccc gcctctgttt tttcaatcat cacaccatag ccatagtagg
     9181 ttttatttat ggtatctgcc agttcctgcc ccctcagcat ttctttttgt gttgcttcgc
     9241 ttaataatgt atggtttttt aaggcccggt cccatttcag cagatcaccg gtggtggagt
     9301 ttacaatgcc gtcaccctga ataccatcga gataaatgac aaaatttgta gtggtgtcat
     9361 tgtcaggtaa aatatatttt ttcgtctcag gcttatatat ataaccatat gcgtagttgg
     9421 caatagtatc ttttaatgag cggcgggtat tgtaaattct tgaatgctgc atgcccagtg
     9481 gcttaaaaat attttcagca agaaaagcag caaaggtttg tccagacacc ttctcgatga
     9541 tagaagcaag gatagcatag gccgtattac tgtattccca tttagtgccg ggtacaaagg
     9601 aagcaggtgg tttttccttt gcaagtaatg caatcatatc tttgttgaat gcaatttttg
     9661 ttttgtccca ttttgcattc atagccgatt catagtccgg caaaccggaa gtatgcgtca
     9721 gcatgtgccg tatggtaata ttggaatagg gcagttcggg aaaataatgc cgcagtgaat
     9781 cagtgagtgt aagcttccct ttttccatca gcagtaatat gcccattgcg gtgaattgct
     9841 tacttacgga tgccagttca aatactgagg aatcattcag cattcttccg gaatcgaaat
     9901 ctgcaaaccc atacgattgc tgaaaaataa tattgccctt ttcagcaata agcacattac
     9961 cattaaaccg gtaatatttc atttggccgg aaaaaaattc gtcagcctta ccggcgaatt
    10021 catttttagt ttcctttttg gaggaacagg cagacagaaa aaatagtact aatggaaaaa
    10081 tttttttaat tgctgaaagt gagtggttgt acattttcat gttaataatt tataatgatc
    10141 ttacaaatgc cagcagggct tttttgtctt cttcatcgag gtataaaccg aatgtgtgcc
    10201 cttttacagc gaaaggtttg tccacattgg ggttaaaacc cgaagggata aaattatctt
    10261 ttaaccgtgc cgggttgaac atttcttcga ggctggtcac atacccgctg tgcatcaggg
    10321 cagaacggtt ccatacaccg atcaaagaag gcactttata atatcccgtg ccccggcggc
    10381 tatacatagc gagatccgga tcggttttta cactgagttc tatgatatca taattgattt
    10441 tatcttcttt agaaggagta aaacctgatg ccggcgtaag tttattactg gaataaagcg
    10501 ggggagtgtg gcagtccaca caggtttcct gttcaaagat ggctttgccc ttggcaatta
    10561 atgcggctgg cgctttgtcg ggattgggca acgactttaa tgaataaagg tattttgcca
    10621 atgcaaatgc ctgggcatct gaaaaacggg tgtacctttt tgcaatgatg ctatcaggac
    10681 tggtgtctgg tttaaatcct ccataagagt tggtaaaatc catttcctgg ttgagtactg
    10741 cataacgcat cacatcaccg atatccctgt gcagaataag accggtgcgg tctaagtatt
    10801 ttctttcttt taaattaaac aggtcaggaa tagccgttgg tgcaccaaag gcactgctat
    10861 gccggtgcat cacaccggga acggttgcca tcaatacatt tgttgcacgt ctccattcag
    10921 gatttttgat gaattcctgg ctctcatgtt ttatccaggg agcgccaaat aaaccgctga
    10981 aggattggct tatcatggca ttggctgcac tgtccggcat ttttgtttct tcggcccggc
    11041 ctttcaatcc tgctccgaat atatcatcaa aaggataatt accctgcccg ccttttaaaa
    11101 cagaaccatc cggcatcact ttgttatgac aattgccgca ggaaaatgaa ccggcttcaa
    11161 ttttaccttt tttacggatc accaaattga aaaagggaat cgttccatct tttgctaccg
    11221 ggacatgcat agcatttaaa aaagatgcca ttccttccag ctcctgcata ttcactgcac
    11281 tgtctataaa cggatcaaaa ttttgcggca tatcataaat gagctcgccg gcttttaccc
    11341 aatcttcttc tgttttaatg gtggaggcat cgaatacgat ttcgggttcc tgttgcaaca
    11401 gccattcata atatcccttt ggttcacgac ccggcagata cattggatac attttatagg
    11461 cattgcgttc aggaatctta taatacagtt cttctggtat tggctccaca tctatagact
    11521 tgtctaccag cggaagatgg gctgatttca attttttaaa gtcccacagc tttggaattt
    11581 cattgttgtt atagaggctt actgaaaaac tcaggaaggc catactcacc atcaccgctg
    11641 taagtagtaa gattgttttc ctgtatttca taaacttttt aatttttgat tttgaatggt
    11701 ctgttataaa gtactggtaa taccagctta gctttcgcca gaatcggtat ttcgtttccg
    11761 catgggctgc cattttttat aagtagcact tcgccataac cattccaccg ggccgtaatt
    11821 aaaatatttc agccaaaggg tactgagtat gacctgggca ctaaaaacag caattccaaa
    11881 aatggtgtaa tagaccggtc ctacctgcca cataaaaccc agcccggctc caagaaatac
    11941 aaagtttcca atcagtgact gggtgatata attactgaac gccatttttc cgacaggcgc
    12001 aaaaacagat aatatttttt tcccggtttt tgtctgaaat actaaaatga aaacacccac
    12061 atatgcaagg gcaagcggtg ccacacccag tgcataagca atggtttgat aaagaccgtt
    12121 cgtttttagc tggaaataat ccctcatgtg gtgctgcata taagttgcca gaaaataatt
    12181 ggccggcaat ccaatgagtg caccggtaat gattatctga accaataatt ttttatgctg
    12241 tagtatatat ttataaaaat ctgaccggcc taccacatag ccgatcaaaa acatgcccag
    12301 cacttttgga acacggctta caaagaacaa atactggtag cgaaaaaaga atccgctgat
    12361 attactcttc aattggtcga accagttacc atttttcatc atatccatga actcctcttc
    12421 gcttttcagg ctcagtaaag catcatctac ttttatccct gttttgatca tcagttcggc
    12481 aggataattc aataccggcc aggtcatctt cagccagtat aataaaatgg gggaaagaat
    12541 aagaatacta cctgtgatca ataaagtttt attagagaac tttcgcaggg gcaggagaat
    12601 aaaagataag agggcataga aaaaaacaat atcacccggc cagatcagca aatgcagggc
    12661 acccagcaac agcataaaga ataatcttct tcttacagta ggtagtgcat taatgccatt
    12721 agcagcagct cttttaaatt gtaatgcaat gccccagcca aataacaggc tgaagatgga
    12781 atagaatttt ccttctatga acagatgatg aaggaatgac ataacccggt ctgcctctgg
    12841 taacaggtaa gggcctgctt cggagcgggg gatttcacgg taccagctga atccattcag
    12901 gtttgcaatg aatataccca ggatagcaaa accccggagt acatccataa atatttcccg
    12961 ttcattggct tttacagggg ctaatgcttg cataagcagt aagttggttt tgtggtttca
    13021 ataaaattgt ccggataggg tacagaagag gtattttaaa tatagtgata gtattttaaa
    13081 cagtcaattt ttaaccggct atacctgtat aaaaattgtt gttgtagatt taatcatcaa
    13141 tacagaacaa aatgtcgctt gatattgccg aaataaaaaa atatttacct gtattcaccg
    13201 aagaattgat ggccgaaata gccaactatg gattgataaa agaaataccg gaaggggtag
    13261 agatattaaa agaagggcag tacatcaaag ctattccaat agtgctggaa gggctcgtaa
    13321 aggtcttcac acggatggaa gaaaaggaat tgcttcttta ttatatcggg cctgcacaaa
    13381 gttgtatcat gtctttttcg gccggcatgt ccaatgcacc cagccgcatt tttgcgttta
    13441 cggaggatgc ctccattata ttgcttttgc cgtcagataa attggggaaa tgggtgaaac
    13501 agtaccctgc catcaatgaa cttttttttc aacagtacaa tctccgctat acggaaatgc
    13561 tggataccat taattcattg ttgtttgata agatggacca gcggttgtac aattacctgc
    13621 tggaaaaaag taaactgaaa ggagagaaga tactgaccgt ccggcataaa cagatcgcag
    13681 gtgagttagg gacagcaaga gaagtgatca cccgggtgat gaaaagactg gaacaggaag
    13741 gaaaagtgcg gcaattgccg gagggcatac aaatcattca ggctggtgac tgatgtcacc
    13801 acaccgcttc agagaggaac tgtagattta cattcaaacc cggaacaatg aataataaat
    13861 ggttcaggct tgcattagtt tgtctcctgt gcctggccat tataaccggg attgtattat
    13921 tgatcaaact tttttaatca aaaaaactat tttattatga aacaaaacat gggaacagct
    13981 gaccgcatta tcagggtgct tattgcagcc gtatttgctt acctctattt cagtggtacc
    14041 gtaacaggca cattaggtct ggtactggtt attgcaggtg gggtatttgt actgaccagc
    14101 ctggtaagtt tttgcccgct gtatacgcta ctgggtatta ctacctgtcc ggcgaagaaa
    14161 caataaaaca aaaaatcaat taagatagtt gaaggagtga gtatatcctg ctcactcttt
    14221 ttatttccag ccaactgttt tgcaagcacc acatcccgtt gttacctcat aactataagc
    14281 ggtgccgggt cggttataaa atacattgtc tgtccatttt tcccagccgc cctggttacc
    14341 accatgtacg gtacctgtag tatatccttt gcctttacaa acagagcaga tactgtaaag
    14401 ctggctttgt acattatata tcttctctat ttcctgttca gtgagtgata cggttttgga
    14461 agcatctttc agggttttaa aagtaccatc ctttctatac acagcctggt atattgcatt
    14521 ggttaccgaa tacttttgtg tagtaaaatc ataaccggta attacagatg taagatagtc
    14581 atcttgcttt ttacgaagag tggtgcctgc tttatattta atttgtttta cagcattatc
    14641 atatgtacta accagtgtag tgggtggcgg ttggttctta tagttggtta ccagctttct
    14701 tgcataaagt cccagttctt tagcatcctg tatatacttg atcttatctg cctcggaaat
    14761 tgtaatatta tcacaaacac tttcgccggc tgtaatttcc ttcaaaaaga tttctacttc
    14821 tttctcataa ccagaataat attttatagg gtcgctgggg agggtgctgg agtaaaagct
    14881 ggtaatcagg tcttgcttta ctgcataggg agataccggg tgataaataa aatacccaaa
    14941 atattttccc ttccatttgt cgtccggtct tttaagagga aagtcaacag tggcccagcc
    15001 atgcaagccc ccatctttaa aagttcctgt catagtacag gataattctg tttcgctgct
    15061 taaacgttta agtacacttt ttccgcagcc atcgaggcta tgatttttaa atgtgccaga
    15121 gaaaagataa ctgttctgtg gaatgtatcc tttagtgtaa ttggggtctg aagagctggt
    15181 gtaccacata tattcgtaaa tactcttgtt gccataaata agccagttgt ttgtaaacaa
    15241 gccagaaagc acatactctg attgccgaag gcaacatgcc caggtttctg ttttaaacaa
    15301 actataaaaa tatcctaatc cgtttgccat tccattttca aaacgactat agaaaaaagt
    15361 attggagttg ttggagaata aaccattacc atgtggctta ccgtctttcc attctccggt
    15421 ataattacca gtatacgatt tttctcccat agtaatggtc aatggtttcg actcagccat
    15481 cgcagcatta ttataatccc aaagcgagga aggtaaattt acttcctgtg aatcttttaa
    15541 aataccattg tcccaaaacc cctggatcgt tttaccatct gtatcaacta atttacccgg
    15601 gccattgtat ttaccatcat aaaaaaaacc ttcgtaccag gtgccgtctt tgaactccag
    15661 ttttcccacc aatggtttat ttttaaaatt cattttgccg gtaaagctct ttgcaggaaa
    15721 attttcctgt accataatct ttgccatttg taactggccg ttctcatatt ttccctgcat
    15781 agatgctgca cccaattccg tcatagcttc tctcgatgca ccaataccat gcagtgtgta
    15841 gctgtaggtt gttttatcgc caatttttgt ttcagtgcgg atggcttcgc cttcggcaac
    15901 tatatatttg ctgccagcaa tagaattatt taaatcgctg gacaggtttt cagcagctag
    15961 cggcgtaaat ttattggcgt aaaaatccct gtttttagca ccactgccgg ataaagtgta
    16021 attatacacc agctttttcc cgttgaattt tctcccgtca tctgagaaag ttcctttgta
    16081 taaattaaca gttagctcaa ggctgccatt atcccggaat tctacctttc tggaattagt
    16141 actggcccaa acaccttccc catttttaca attgccttct atgcaatcac tcagaaaaaa
    16201 ctggtagata atatcacctt cgctggccct gtagttcagg tgaaaatttt tgatagcaat
    16261 atccccggtt ataggccaga agttttttgc ctttatctgg gcactgctat cttgttggta
    16321 aaagaaggta gaacataaaa acaggaaaaa aagcttacgc atagcgaaga gatttaccct
    16381 taaaagtata aagatacctg cagccggaaa tacgggctgg cgggtatttt aatcttcttt
    16441 ggaacccttt tctcccacaa accaatgctc tttattttta aacagtacat tgaaggtggt
    16501 aagggaacca tagcagcggg tgatatattg ctggatattt actttctctt catcattcag
    16561 cgttttgctg ctgttgatat tttgctccag cactcttaac ctgtcccgca gcataacgat
    16621 cttgtgaaag aaatcctcaa taggaatttc ttttggcttt aagcttttat ctgcaggctg
    16681 taacaacagt atgcctttta tccatttatc accgagtggt acagtttctg taaggccgcc
    16741 ccatagccgg agtattttta ataaagacat ttccacttcc gagtgggttt ctatctctgc
    16801 cgatacattc tcgggaatga tctcttccag cttattatcg gtcttgtcta tttctttgat
    16861 accatgattg ataaatgaga tcaggtaagt ggcatatctg ataccaacta tcacacccgg
    16921 accgaaatgg gtatgctgca gtcttgttcc gattcccaaa gttgtttgct ccataattca
    16981 ttcaaattac aatgcagggg tgaatttgcc aagaacttat acgtaaacag tgccaatgtt
    17041 cccggttctg tttttattat ttgaataaaa atgtatttta ggtatgtatt tcaccttaaa
    17101 tctatccaca tgaaaagaat atccttcctg ttaatcctta ccggatttgt agttcctgtt
    17161 ttaagccagg taaaacaatt gcctgttcaa aaaaaagatg aaataaaagt tgtaacaact
    17221 gtctctcagc cggtgggtgt aatcgccatg cggggttttg attttgaaag aggaaaagat
    17281 gcattggctg actggacgaa agaaggaaca gctttcaata atcaacctgt ttacgggcat
    17341 aatgttatta tacgacgtga aatggataat acatataatc cttttcggat caatatttca
    17401 ctgggtggtg attactggaa agatctctct cttccaatcg ggcataaagg aaattactgg
    17461 atcggcagtt atgaaaaccg gagcgactta acgcaacctt ttggacgaac acagggtaat
    17521 attccaacag gagtgcttat ctctccggag tttactatca caaaggatta tatcagtttc
    17581 ctgattgggg gcacaaatga cctggccaat gcaaaagtag agttggggta tgaagggcag
    17641 ggggaaactg atacagaaga gaaaaaggaa aaagatcctt tccgtgtaat agctccggtg
    17701 attaaaacaa tacccgtagt aaaacaattc cgggcaaccg gcaagggcag cagtgtaatg
    17761 agaagggtgt ggtgggatgt gaagacatta aaaggaaaaa aagcaaggat aaaaattact
    17821 gataatactt ctacaggata tatcaatgca gacgatttta ttttccagga tgcagcgccg
    17881 gttgataccg acatcgccat caactctaaa gtaaatgtga agcaggtatt tcaaaaagag
    17941 ggcaattatt atgactggga ttttccttta tggggtacca ttgacacaca tacgcatccg
    18001 gcatcacata tcggttttgg caaccggtta ttccatggag agcctgttgg tactatcaat
    18061 aaagcactgg cagattgtga aatcgggcat ggtgctgata ttttcagaac actcacaagc
    18121 agcagcgggg aaaggatcag cggtaatata atacgcaatg ctgtggccgg ggcatttgat
    18181 ccgggacacc ggctgggcgt aatggatccc ggcaatacat ggggtagtaa tgaacaaact
    18241 ctggcatact ggccattgta caacacgaaa cttcaccagc aaatgtggaa agactggctt
    18301 aaaagagctt ttgatggagg agtaagaatg atcgtggcgc taacggtaca tagccaggtg
    18361 tttgcaagaa tcagtgaagg tacccgcccg gatgacgata aaactgttgg tgataaacaa
    18421 atcgactata tcaaaaacgt aattgctcct gcttgcaggg attggatgga aatagcctat
    18481 tctcctgctg atatgagaag gatcatgttg tcaaataagc tggcagtggt attgggcagt
    18541 gaattggata atattggaaa ttttgacggg aaaagaactc ctaccgaagc agaaatcagg
    18601 gcagaaataa gaagattgta taataaagga ctccggtata tgttcccggt gcatctatta
    18661 aataatgggt taggtggtac ggctattaag tcagatatat tcaatcttgc caccaagttt
    18721 aataccggaa gaccttttga tgtggaactg aaaaattata aagatggttt ctcggaatat
    18781 aaactcaaag cgaaatttga tgtattaagc gatatgccgg cagccattat tgatgcgaat
    18841 aataaagtgg ctgcttatgc cgggattgca ggtgctgcag ctccgcttgt tctgcctgct
    18901 gtggctccgg ctatcctgcc gtttattccg ttaataacaa cagttggtac ttccgtagtg
    18961 ggtatagcag cgcctttgat ccctattctt gccacggtgg ccatcaacaa atggaccaat
    19021 gaatggggta tatcaatgga tgtaatccct gtaggcaata attatcctgc ctatccctgg
    19081 aaaagatatg ccgggcatat caataaacag ggactaacta cagcaggtga aattgcagtc
    19141 aatgaaatga tgaagctcgg tatgatgata gacattgatc atgccggaag aaaaacggtg
    19201 gatgattatt ttgaaaaggc tttcgctgtt cccggcggtg gttacccgtt gaacagcggc
    19261 cataaccagt ttacagaaac ccggtggaca gaagctcaat tattaccgga ggagtatgat
    19321 ttgcgttcag aagagggacg aaaattatac accaatcatt acaatcatat agctgaaaag
    19381 attgccaatg agaatgaccg gaaacctgat gagctgagaa aaattaaaca attgggtggt
    19441 ataatgggag tgggtatttc cggggctaat gtgaatgcta tttccaatat aataccagct
    19501 tccggggcca ggtatattga atctgtagtg aagaatgata atcccggcag ttcaaaatct
    19561 gccgctcagt tattattata tgcaattgaa aaaatggaag gcagcggtgt ttgttttgga
    19621 acagatatca atggctttgc cggcctggtt ggaccaagat ttggtgccaa cagtgccagt
    19681 gctgcggggc acgacaggtt cgatatatat aacaggcaaa gttttgttta ttcccaggcc
    19741 aaccaggtta aataccgttc cttcacagat ttaaccggta acaggttcag gggtcatctt
    19801 accggctcct atgatgaatt aaaaagcagc ccggacagat ttttacagaa tggaaagtta
    19861 tttacagatg agcagggaga ttttatgctg gccctccaga tatattattc catggaagaa
    19921 cgattaaaaa aagtagcaga atccggggat accaaaaata aaatagactg gtacctcaat
    19981 aacgttgtgc ataatagtta taacaaaggt aggatcaggg aattcattaa agggttgatc
    20041 aagggaggca atgaggaagg aattggaaca gatgtattta acggtgatgt gggaacgcag
    20101 caacaaattg cctgtaaagt aagcagggaa aaaatattta ataaaagaac atttgacaac
    20161 agggttgctg atgtggcgat caatgctgaa aaatacaaaa gacataaatt actggagcag
    20221 gcgttaaact attataaaat aatgatgaaa cccggtacga atgaaccctt gataccctcc
    20281 tccacattgg gtaaaacatg ggattataac ctggatggga tggctcatta tggaatgatg
    20341 gccgatttct tccaggattt aaagaacgta ggcatgaagc ccaaagacat gtccgtacta
    20401 ttcaactcag ccgaggattt tgcccggatg tgggaacgtt gtctccggca aagtgagaga
    20461 gtcaggtaat acaaacgaga gtttgacaat acaaaaaaaa gaatgctgta tttatttgta
    20521 atcaataaca ggctgcaatt tattgctcct ccagctcttc tttataccag gcatccggtt
    20581 gcatccctct tttgaaccga tagagagctt gtttgatgta caacctgaat tcataatcac
    20641 ctgccagttt ggtaaaagtg taggaaggtt taaatacccg cataaaacta tccagttctg
    20701 ccccgtcaag taatgttaat cggcgcacca gcgctttgtt gaaacggaag tccacatact
    20761 tatcctgttc ctccattaat aatcttcgct ggaaagatgc catactccgg tttcgcttca
    20821 cccggaacat attgatgatc tcatccaggt caaaaccaac tgcggctcca tattggggtg
    20881 ttacagtttt caaagttggt ttctggtaat taaaaatttt agcatagtcc tggcggttcc
    20941 ggagagaatc cagccggtag ttcctgggta atatttttac ttctttcatt accggcacat
    21001 ttacctgtaa tgaaatatca aactgcatcg ggttgccgat cctcattacc gggaacttca
    21061 ctgttggttt attgagatag ctgaaccaga ttgaatcttt ttcagtcact tcaatttcat
    21121 aatatccatc agcattggta acggtgcctg tacccgaagt ggagagcaca cttaccagtt
    21181 ccataggata attcctggaa ctatcataca ccacaccttt tatcttatag gtctgggcaa
    21241 tcacagatgt ggccgtaaaa aggaatgaaa taatgaataa tatatatatg gggtgcttca
    21301 caaaaattaa tcagcggcaa gtaacaaaag aaaatgcaaa aagaagtgta aaatcaaaat
    21361 gtcgtatgag tcaataaggc cggatgacag gtgaaaataa tttttaagtt gctttgtttc
    21421 ttatttgctg ttgttgaaaa ggatctcttc aatgacttta ggataatgtt catgttccag
    21481 ttgctgcact ctttttgcca gtgaaaaagc tgtatcattt tcaaataccg ggcaggtagc
    21541 ctggaatatt ggttgcccat ggtcatactg ttcatccaca taatgaatag taattccgct
    21601 ttctttttct ttattatcaa taacagcctg gtgtacatga aagccgtaca tacctttacc
    21661 accgtatttt ggcaataagg caggatgtat attgatgatc tttccgggaa atgtttttat
    21721 cagtaaagca gggatcttcc ataagaatcc cgctaatacg atgagatcaa tgccctcttt
    21781 ttttaaatca tctgcatatc ccgatcctat cagaaatttt tctttttcga taatcagcgt
    21841 gggaatggtg tttttttcag caatcgttac tacaccggct tctggcttat tgcttactat
    21901 cagtgcaatg ctggccaact gggaattatt gaaatactca atgatctttt gcgcattact
    21961 gcctccacca gatgcaaaaa ctgcgatctt attcatagta tagtatcata atgagaaagc
    22021 accaggaaag tctattcatt tcttttccct gatgacaccc attttttgtc ccagtttatg
    22081 gatataagca ttgaaaaaac ggaactggcc gaatggaatg cttacaatta ttaccatcaa
    22141 tggccagata agggtcacca gcagaatata aatgactgcc cagagccaat cctgtccgat
    22201 ggacagcgca ttcataatct tttttcccac gaaacccgta gcactgcctc cgatggtaaa
    22261 agtgcaaaga atcagagcca gctgaaggcc attcaccttc cattttttct gtaatcgttc
    22321 aaacatgcag tatcattaaa attaagcgac gcaaaagtca ggaaaaaatg gccatgaaag
    22381 acgactattt tcacatctgt tgaactttat ggaaaggttg accgatttat gacaaacaac
    22441 tttactgttt gctaaaaaat agtaataaat ccgctgaaat gcaggctggt agcggattaa
    22501 aaaaaaagtt aagtacttgg cgttttgtta agttcctgac ttatttttgc gcctgattag
    22561 agctatttat ccacattttt cagatcaatt gcatctatga ttctttgtaa accaatcggc
    22621 cagagactga catctttgtt tctatctttt tttgtctttt ctatagtgag tgtaactgca
    22681 caggatattg ctgcaggcaa ggcgatattt aacagtaaat gtgctgcctg ccacaatgtg
    22741 ttgaaagacg ggacagggcc cgccctggcc ggaattgagg aaaggcacaa atgggctgat
    22801 cataaagaac tgctggcatg gatcaataat cctgcatctt atatggctaa agacccttac
    22861 actcaagggc ttaaggctaa atacggatca atgatgacag gttttgctga tgtaaccctg
    22921 gctgatgttg gaaatctggt gtcctatata aatgaagccg caaaaccagc tccaccgcca
    22981 cctcccggtg ctgaagtaca gccatctggt aaccagaatg ccatcatttt tggtgtaatc
    23041 tccctcatat taggtatcat agcccttatt ttaatgcagg tgaacagcaa cctgaaaaaa
    23101 atgtcagatg attcggaagg gattattcgt ccggagcctg ttcccttcta tcgcaataaa
    23161 gtatatatcg caatggccgc cattattttc ttcattttcg gtggttatat ggtagcgaaa
    23221 ggagctatcg gactgggtcg tcagaaagat tatcagccgg agcagcctat ttattattct
    23281 cacaaagtac atgcaggcat caaccagatc agttgtctat attgtcatgg ttcagcatgg
    23341 gacagtaagc atgccgctat tccttcggta aatacctgta tgaattgcca caaagccatc
    23401 accacttacg aaaaagggcc aaagctctat gatgaagctg gtaatgagat caatggtaca
    23461 gcggaaatag ccaaactgca tgctgcagca ggatttgacc ccaacaatgc aaataaatgg
    23521 gatccttcca tgatcaaaaa gccgattgaa tgggtaaaaa tccacaacct gcctgatcat
    23581 gtttatttta atcactcaca acacataaga gccggtaatg tacaatgcca aagctgtcat
    23641 ggtgaaataa ctgcaatgga tgaagtgaag caggtggccg aattgagtat gggctggtgt
    23701 atcaactgtc atcgtcaaac aaaagtggat tttaactacg acagcaccaa aggcaataag
    23761 tattacagca tctatgagaa gtttcacaac gatttcaaat ccggcaaaat ggatagtata
    23821 actgtgaaag atatcggtgg actggagtgt caaaaatgtc actattaata aggttagtta
    23881 agaagttgaa aaactcgaat tagtaatcag cacattatcg aattatcaaa ttgatattca
    23941 tgagccaaaa aaagtactgg caaagtttcg gagaggtgaa tgacaacgaa aactttcaaa
    24001 agaagcagca ggatgagttt aaagaagaat tgccatttga aggtttcgac gacaaaggat
    24061 tgattgatgc caaagctccc cgcagggatt tcttaaagta tcttggtttc agtacagctg
    24121 cagctacgct ggctgccagt tgtaaggttc ctgtaaagaa agctatacct tatgccaaca
    24181 aaccggagaa tcttgtaccc ggtgtggcta aatattatgc aaccacttat gtgcaggatg
    24241 gtgatgtggt accagtggta gctaaagtac gagatggtcg tcctatcaaa atagaaggaa
    24301 atgatctttg ctcttataca aatggaggca ctacagcccg tacacaggct tcagtgcttg
    24361 atctgtacga tacacatcgt cttcgtttcc cccagcatag agtaggtaaa ggagcggatg
    24421 caaaatttga agaagtcccc acatttgagc agtttgataa aatggtgggt gatgcgataa
    24481 aaaatgcagg tggtactact gtattactga ccagtactat tgtttctcct tctacaaaag
    24541 aaataattgc taactacccc ggcctcagac atgtacagta tgatgcagtt tcgtacagtg
    24601 gtatgttact ggccaatgaa gcaagtggtt tcggaaaaaa aataccaacg tatgattttg
    24661 gcgctgctaa ggtaattgta agtcttggtg ctgatttcct gggtacctgg ctcaattcag
    24721 ttgaaaatgc caaaggctat tccgttggcc gcaggatcga cgagaaaaac ccttcgatga
    24781 gtaaacacta ccagtttgaa ggctacctga gccttactgg tgcaagcgct gatgaaagat
    24841 tcacacaccg tccatctgaa acaggtcttg ttgcactagg gttggcagtt gcagtgggtg
    24901 cagttgatat ggcttctccg gttacggatg caaaactaaa agcgggtatt gaaaaagtag
    24961 cagctgatct aaaagccaat gccggcgcag gcctggtggt ttgcggcagc aatgataaaa
    25021 atatacaggt aatcgttaat gccatcaata atactattgg agcttacggt aaaacaattg
    25081 actggagcaa accggttaat taccgccagg gcatcgattc agattttgca cagttagtgg
    25141 cagatatgga agccggcact attggaacta ttttaatatg tggtgccaat ccggcctaca
    25201 actatcatga tgccgcaaaa ttttctgctg cattaagcaa agtaaaagtt accgtttctt
    25261 tcaatgaaag actggatgaa acaactgaac tctgtgaata tgttgctcct tcccatcatt
    25321 acctggaaag ctggggtgat gctgagccta aaacaggtgt ggttagcttt atgcagccca
    25381 ctatctatcc tttattcaaa acccgtcctt tccagacctc attgctgaag tggagtggta
    25441 ataatacaga ttacgaagtt tattttaaaa attactggac tggtaagctg ggttcagaag
    25501 aggctttcaa taaaattttg caggacggta tcaaagaaga agcccctgca gctgctggtg
    25561 ccggtaccta caacggagga gcagttgcta cagctatttc cgccattgaa tctgcaaaga
    25621 aagttacagg tgctgaagta gtgttatatc aaaagacatc cattggaact ggcactggtg
    25681 ctaccaatcc ctggttgcag gaattaccgg atgccatctc aaaagcaacc tgggacaact
    25741 atgcattgat cagcatgtcg atggcaaagg aagtgcttgg gctggacctc atcaatggat
    25801 ctgaaaagga aagcaacaat tacgaatact acccgcaaaa gcctgttatt aaattaacgg
    25861 ttggtaaaaa agaagtggaa ctgccagtat tggttattcc cggtatgcag gctaatacta
    25921 ttgctgtagc tgttggttac ggaagaggcg agaagttggg taaaacagca gctggtatcg
    25981 gtaaaaatgt ttatccattc acctcctttg atggcactac catatcgtat catgcagccg
    26041 taacagttgc agatgctaaa cgcaaagaaa aaattgccca gacacagatt cataattcat
    26101 acgaagaccg gattgaagta gtaagggaaa ctacactggc tacattcaaa aagaatccca
    26161 atgttattcc tgattaccgt aatcatctcg aagagacgta tggtaaaagt cagaatggag
    26221 caactactga ttatagaaaa gaaggaacat tgtatccact gcatgatcag cccggcatca
    26281 agtggggtat gaatatcgac atgaatgcct gttatggttg tggcgcctgc gtagtggcct
    26341 gtcatgctga aaataatgta ccggttgttg gtaagagtga agtgcttcgt tatcacgata
    26401 tgcactggtt gcgtatcgac cgctactttg ttagcgacga taaaaatcct gatgagctga
    26461 aaggggttgt gttccagccg atgatgtgcc agcactgtga taatgctcct tgcgagaatg
    26521 tttgtccggt tgctgctact aaccacagcg cagagggaat caaccaaatg acctataacc
    26581 gttgtatcgg tacaagatat tgtgctaata actgtccttt taaagttcgt cgctttaact
    26641 gggcggatta cacaggagca gatagtttca aagacaacca ggatcagcaa ttggtcggta
    26701 aactggatcc tgttgtgcac cagatgaatg atgacctgac aagaatggta ttgaaccccg
    26761 atgttaccgt acgttcaaga ggtgtgattg aaaaatgttc attctgtttc cagcgtttac
    26821 aagccgcaaa attggaagcc aagaaacagg accgtccgtt gaaagatggc gatgctaaaa
    26881 cagcctgcca gacagcttgt tcagcaaatg ccattgtatt cggtaatgtg catgataaag
    26941 aaagtgctat cacaaaagtg agagcagaaa atacacagcg tggtttttat gtgctggagc
    27001 aactgcatgt gttaccgaat gtgagctacc tggctaaagt gagaaataca gaagagatag
    27061 ttgaaagtga agggcatcat ccaccaccag catcagaaca tggtgcagag gggcagggtg
    27121 aaaagaaacc ggcagaagca cagcattaat ataaaagcag aaagttgact aactatatcc
    27181 ttagaatccg ataaagagga caaggacaaa caaccagaat atggcattac ttagatacga
    27241 atcacaggta agagcaccat tggtagatgg caccaaagat tatcaccagg taacggaaga
    27301 tatttgccgc ccggtggagg ctaccccatc taaattatgg tggatcggat ttttaatctc
    27361 cgttgcacta ctcatcttcg gtattatctc tgtaacgatg gaggtaatat atggtaccgg
    27421 tcagtggaac ctgaacaaaa cagtgggttg gggctgggac atcaccaact tcgtatggtg
    27481 ggtgggtatt ggtcacgccg gaaccctgat ctcagcgatc ttgttattgt tccgccaggg
    27541 atggagaaca ggtgtaaacc gtgccgctga agcgatgacc atttttgcgg taatgtgtgc
    27601 cggtcagttc cccatctggc acatgggtcg tgtgtggaat gcattctttg tacttcctta
    27661 tcccaataca agaggtcctt tatgggtgaa ctttaactca cctctgttat gggacgtatt
    27721 tgccatctct acctatttca ctgtatcact tttattctgg tattcaggat tactgcctga
    27781 tctggctacg ctgcgtgaca gggcaaaagt gaaatggaga aaagcttttt atggagtagc
    27841 tgcatttggc tggacgggtt ccaccaagca ctggcagcgt catgaagcat tatcattagt
    27901 attagctggc ttaagtacac cacttgtact atcagtacat acaattgtat ccttcgactt
    27961 cgccacctcc gtagtaccgg gttggcatac aactatcttt ccgccttatt ttgttgcggg
    28021 tgccatcttc tccggttttg cgatggtgca aacgctgttg gtggtaacca ggaaagtgct
    28081 ggcattggaa gattatatta cgctggagca cattgaagta atgaataagg taattgtgtt
    28141 gacgggttca attgttggta tcgcttattt aacagaatta ttcatggcct ggtactctgc
    28201 agtagggtat gaagaatttg ccttttttga aaaccgtctg aacctgaaca gtccctatgg
    28261 ctggagttac tggatcatga tgggatgtaa cgtattatct ccgcagatat tctggttcag
    28321 caaaatgaga aggaacctgg tggttacatt cttcatgagt atcctgataa atattggtat
    28381 gtggttcgaa cgttttgtga tcatcgttac ctctgtgtac cgcgactacc tgccatctgc
    28441 atggagtaca tattatacac ctaccatctg ggaagtggga ttctatttgg gaacatttgg
    28501 tttgttcttt acctgttatt tcctgttctc taaattcttc cccgttattg cactggcaga
    28561 gatcaaacat attttgaaaa agaatggtga gcattataaa gatcatatgg atgagattga
    28621 agaggagtca actgaagaat ttggccagaa acatgggcat gatcacggag cgtagaaaag
    28681 gaattagtta tcagttttat tgctgataac tatgaatgat aaacattggt ttaaacgata
    28741 aaagctagca gctatgggct agcgcaaaaa gcttaaaaaa tggctgtaaa aaaatttgta
    28801 gtcggcaatt ttgatgatga agcggtcctt tttcctgcgg tgaagaaagt tcgccgggca
    28861 ggatacaaga ttcacgatgt gtttacaccg tttccgattc atggattgga taaagtgatg
    28921 gggcttcgtg ataccagcct gcatactgcc ggttttatct atggtataac cgggacagct
    28981 actgcggtag ggttcatcac ctgggcattg acagtagatt ggcagataaa tttcgggggt
    29041 aaacccttct ttgccctccc ggcatggata cctgttacat tcgagctgac tgtattattt
    29101 gccgcggtag gaatggtatt gactttttgt tatctctgtc agctggctcc ttttgtaaag
    29161 aaagaccact ttaatccccg ttccacggac gatatatttg taatggcgct ggagtgtaca
    29221 gataaaacaa atgaagcaga ggcgatgagt ttcctggaaa caatcggagc gaaagaagtg
    29281 aaagtggaat ataaagaaac agaatggtgg ctgggccgct atgataaaga caggatgaga
    29341 tttcagaaaa aagagcaaga caatcattaa tcgtaatact tagagataat gaagaaaata
    29401 ttcatagttg cgggttgttt ggtattagca gttgcaatga tcagttgcaa taatatcagg
    29461 cgtgatgaag gccgtgctta tatgccggac atggcttaca gcagggcata tgaaacctat
    29521 gcttcaacag aagcattgga taaagctggt gtaaattatg tctcaaagcc cgtagacgga
    29581 actattgcca gaggagagat ggcagcctat ccttttaaga atgattcaat tgggtatgta
    29641 aggtctgcgg aggttaaaaa tccattagat attacagcta ttgacatgaa agaggcagaa
    29701 agattgtatt tggtaaactg cggtatctgt catggcgcta agctggatgg taatggtcca
    29761 ttgtggaatg gtggtaatgg tgctttcccc aatgcgccaa gaaatctgct gacagatcct
    29821 cttgttgcca acatggcgga gggtacaatg tttcattctg ttacttatgg aaggaataac
    29881 atgggaggct atgcttcaca aatgagtaca aaacaacgct gggaagtggt tgcgtacatt
    29941 aaagcaaaac aaggacaagg gagtacagtt gtggctccgg cagcagcacc atcagatagt
    30001 acagctgcgg taaaaaaata aaacagtttt tgaagaaaca ataaataata actgatgtct
    30061 atccgggaaa attttgtagt accaaaacga tacaatacaa tagcttttgc attgatggct
    30121 attggtattt tggcgatcat tggtttatat gttaccagcg gatctaaaag tgacccaaaa
    30181 gagcaggccc gtttctgggc atccttgctc caaaacagtg ttttcttcct gctggtggtt
    30241 aatgcttcta tgtttttcat ttgtgctact acactcgcct ggggtggctg gcaaatggct
    30301 tttcgccgtg taccggaagc aatttctgct gtagttcctg ttatcggggt tatatgtggc
    30361 gctatcttac tggcaatagt atttggaggt aatcatacga tttatcactg gacggatacc
    30421 catcatgtag cggaagacgc tattttaaat cataaaaaag gatttttaaa taaagggttc
    30481 tttgcagttg ctactgttct taccattgta ttatggtcag tattaggctg gaaaatgaga
    30541 caattgtccc gcagcctcga tagcaagccg ctggctacgg tagaagcaag aaaaaaatat
    30601 atctggaaca atacagtttg ggctgcgtta ttcattatag tgttttcatt aactgtcatg
    30661 tcttccatcc cctggttgtg gctgatgagt attgatgcgc actggtacag cactatgtat
    30721 agctggtata cgtttgccag cacatttgta tccggcatgg cattgatcgc attatttgtt
    30781 gtgttcctga aaaataacaa ctacctggag tacaccaata atgagcacct gcatgatatc
    30841 ggtaagttca tgtttgcctt ttctgttttc tggacctacc tgtggttctc ccagttcatg
    30901 ctgatctggt atgcgaatat cccggaagag accatttatt tccagccccg ggcacatgga
    30961 atttataaag gaatcttttg gctgatgttt ataatcaatt ttattgctcc tttgttatta
    31021 ttaatgacaa gagatgccaa gagaaactat ggaacaatta catttatgtc gttgttaatc
    31081 atatttgggc actggctgga tttttaccag atggtatttc cggcggtgtc tccgaacaaa
    31141 gtaccgatga tattgtatga tttcggggtg gcattaggct ttgtggggtt gatcatgttt
    31201 gtaacgggca gggcgttgag caaggcaccg ttgctggcaa aaaatcatcc ttttgttaaa
    31261 gagagtttga ttcaccatac ataaaggaca gaagttattt acgtttaatc aggtagaaaa
    31321 aactgaatta tagattatgc agacattttt catagttgca attttagcgc tgggatttct
    31381 gatcaccttt cagattgcca aggccagtga atatgtagcc gtgttgcgag gatatgaaaa
    31441 agcccgcaag caaaccaata agatcaatgg ctttatgttg ctggcctttc ttattattgg
    31501 attgatcggt gtgtattatt gtaatgaaaa gctgaaagga aagattcttg gggagcctgc
    31561 ctcagaccat ggggtactgg tagacagaat gctttatgtt accctgttaa tcactttcat
    31621 cgtatttgtt atcacacaag ttgccctttt ctggttttcc tacaaatacc aggaatcaga
    31681 taaaagaaaa gctttttact ttgcgcatag taacaagctg gagttgatat ggaccgttat
    31741 tcccgccatt acacttacaa ttttggttgg gtttggtatc ttctactggt ttaaaataac
    31801 tggcgatgct ccaaaaaatg ccatgcaggt ggaagtggtt ggtaaacagt ttggctggga
    31861 gtttcgttac ccgggtagag acaagatcct gggtaagaaa tatttcaaga atgtaaatcc
    31921 cgcagccaat aaccctgttg gtcagatttg ggatgatcct gcaaaccatg atgatatatt
    31981 cgttgaacag gaaatgcacc tggtagttaa taagccggtg aaactggtaa taggtgccaa
    32041 agatgttatt cacgatgtgg gtctggctca tttcagaatg aagatggacg ctgtgccagg
    32101 tactcccacc accatgtggt ttacacctac ttacactacc aaacagatga aagaaaaaac
    32161 agggaatcct gatttcgtat acgagctttc ctgcgaccag atgtgcggta aaggtcactg
    32221 gagtatgaga ggaacgatag tggtggagac acaagaagaa tttgatgcat ggcttgcatc
    32281 taagaaaccc cagtatttag tggccaatcc tgataaagat ccgtcagctc caaaagctga
    32341 tactgtaaaa caggtagcgg ctgtaataaa ataatagttg aagaaggtaa gcatcctacc
    32401 cctggtgcat tactaacata aaattttcct ccatggggat ggaggcaata aaataagact
    32461 atgagcgaag caatgaatgt acattccgga gtggacgctg cacacgcagt acatcatgat
    32521 gatcatggac atggccatca tgagcaatcc ttcatttcga agtatatctt cagtatggat
    32581 cataaaacca tcggtaaaca gttcctgatt actggtatgg tttgggctat tatcggtgga
    32641 ttgttctcag tgttattccg attgcaattg ggttatcctg atcaatcatt cccgatactg
    32701 gaaacttttt ttggcaattg ggccaaaggc ggtcagatac aacctgaatt ttattacgga
    32761 ttggtaacta tgcacggaac cgtgctggtg ttctttgtat taacagccgg tctaagtggt
    32821 acgtttgcca acttactcat tccgctgcag attggtgcaa gagacatggc ttcaccattt
    32881 ttgaatatgc tctcttactg gtttttcttt attgccggta ttgttatgtt agcttcgatt
    32941 tttgtacata caggtcctgc ttccggcgga tggaccatgt atccgccatt gagtgcatta
    33001 cgccaggctg gtgatggaca aaaaataggt acagacctgt ggcttataag tatggctttg
    33061 ttcatcgtgt cttctttatt gggaggcctt aactatattt ctactattct gaacatgcgg
    33121 accaaaggga tgagtatgac ccgtttgccc cttcctatat gggcgatgtt ctttactgca
    33181 gtgttaggcg ttttgtcttt ccctgtatta ttgtctggtg ccatcttatt gttattcgat
    33241 cgtaatctgg gtacaagttt ttacctgagt gatattgtta tcaatggcga gatattaccg
    33301 aacgaagggg gtagtgcaat tctttaccag cacctgttct ggttccttgg tcaccctgaa
    33361 gtgtatatca ttctgttgcc ggcaatgggt atggtatctg aaatactttc tgttaatgca
    33421 aggaaaccaa tctttggcta catggccatg ctgggttcct tgtttgcgat tgccatactt
    33481 gcattcctgg tgtgggcgca ccacatgttt gttacggggt tgaatccatt ccttggatca
    33541 gtattcgtgt tgctcaccct gctgattgca gttccatccg caatcaaagt atttaactgg
    33601 cttactactt tatggagagg taatatccgg tttactccgg
//
LOCUS       DAORTI010000022        33603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_857_length_33603_cov_8.242071, whole genome shotgun sequence.
ACCESSION   DAORTI010000022 DAORTI010000000
VERSION     DAORTI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 33603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33603
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_33603_cov_8.242071"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLO70_04900"
     CDS             <1..529
                     /locus_tag="PLO70_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQZ73827.1"
                     /translation="TGKNVQKHSNDTVRPPVPFYSLKGESGNGDTIDFNSFQGKKVLL
                     VNTASNCGYTNQYDDLQQLSEQYKGKLLVIGFPANDFKEQEKGTDEEIMQFCKLNYGV
                     TFPLMKKSVVIRSAGQNPVFQWLTDSAKNGWNNKYPSWNFSKYLVNENGILTNYFDPS
                     VSPLGKEIREAIEKK"
     gene            538..1047
                     /locus_tag="PLO70_04905"
     CDS             538..1047
                     /locus_tag="PLO70_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73828.1"
                     /translation="MFTKLDIEAYFTGEKQESLLFLFIGIAGISTAIIFFFLLKTSFY
                     LGAAIPLLVIGLLLGIVGYTIYKRSDDDRKRNVYAYDMNPSALKEKELPRMKKVMKNF
                     VIYRWIEIFLFLLGTGLYICFIRDFKHDFWRGFGLALAVMALLALAADYFAEKRGSIY
                     LKGIESFVQ"
     gene            1130..1372
                     /locus_tag="PLO70_04910"
     CDS             1130..1372
                     /locus_tag="PLO70_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73829.1"
                     /translation="MTVADIIGTIGVGLILIAYFCNTFGWISGKGKLFFLLNAAGAGL
                     ACYASWLISYWPFVVLEGTWFLISVVGLARTFKVKD"
     gene            complement(1369..2409)
                     /gene="dusB"
                     /locus_tag="PLO70_04915"
     CDS             complement(1369..2409)
                     /gene="dusB"
                     /locus_tag="PLO70_04915"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQZ73830.1"
                     /translation="MVTIGNIQLPEFPLLLAPMEDVSDPPFRAVCKDNGADLMYTEFI
                     SSEGLIRDAIKSRKKLDIFDYERPVGIQIFGGDEDSLALAAKIVEVTNPDLLDINFGC
                     PVKKVALKGAGAGVLKDIDLMVKLTSAVVKSTSLPVTVKTRLGWDDNNRNIEEVAERL
                     QDAGIKALAIHGRTRTQMYKGEADWTLIGKVKNNPRIKIPIFGNGDIDSPEKALEYKN
                     RYGVDGIMIGRAAIGYPWIFNEIKHFMKTGEHLPAPTIEDRLFVCRKHLRKSIEWKNP
                     VVGINEMRRHYANYLKGLPNIKEFRNKLVTLKTEEELNDVFENIASTYKGYEFEKTPI
                     ELVNYHEKCPVN"
     gene            2492..3442
                     /locus_tag="PLO70_04920"
     CDS             2492..3442
                     /locus_tag="PLO70_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQZ73831.1"
                     /translation="MKGLLKTKSAGNQFLLLVSISLVSFFLVGLIGTVILSALTGMDI
                     KEMSDMTKWDYSKPATINFIRGMQLIQFISLFLIPTFICARLFSTDTRQYLGLRKPFT
                     RSYWVIGILVMILAIPFVQWLGELNRGIRFPSGIEHWMKAKEAEANKTVMALLSRQTI
                     KDLILNIICIAGLAAVGEELLFRGMAQRLLIKMFKSPWPGILIAAFLFSAMHLQFYGF
                     FPRFILGILLGIVYWYSGSLWPAILAHFVYDALLITLAYFNPSMLNEEATVKLSSIAL
                     TAAISFIMLALAVNWMMKNSKADYNQVYAEDEVPVKDHPF"
     gene            3457..4353
                     /locus_tag="PLO70_04925"
     CDS             3457..4353
                     /locus_tag="PLO70_04925"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQZ73832.1"
                     /translation="MAFNWQTFKTRALTAVVFVVVMLAGLLWNHWSFFILFSIIHFGC
                     WTEYQKLVGLIDPGYKEITPFHKYGVMLAGWCLMLWFSGDDLAIGGLRLHEMGWWIGL
                     VLVFLLPITELLFSKNIRLKNIAHSVFGLIYISLSWGLMMDMYDHPAAVHIHNTTFVI
                     ESGIFPIIIIISIWINDTMAYIVGSLIGKTPLSPISPKKTWEGTIGGILLAIAVVGFL
                     FPFFYFGSSAFALFMFIAPIAAIAAIVGTYGDLLESKLKRLAGVKDSGQIMPGHGGFL
                     DRFDSLLLATPFVWLYVQLFMK"
     gene            4383..5066
                     /locus_tag="PLO70_04930"
     CDS             4383..5066
                     /locus_tag="PLO70_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73833.1"
                     /translation="MGKLICSLSLLFITSSAGAQRIFSDGNWGATLAGAMVPLPTFTL
                     GVQPGVLYKISDRVSLLTELTIPVVKNIGDNSGTTNQKYFRIQPEFRYHFPFNMRRNN
                     IYMGLRLSYAIRKFEDADGGFYSDVTPGFDEGYYFDKAQVNSPIMTSSLQVGTITNGN
                     KKITTDVFWGFGVRYINTEYTDVVNPVSGIRNRPADGPLFYPSYSYNRAVTWLHVNGG
                     FRLIYHFGR"
     gene            5115..5771
                     /locus_tag="PLO70_04935"
     CDS             5115..5771
                     /locus_tag="PLO70_04935"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQZ73834.1"
                     /translation="MTIHKEGYKTIAISTIVFGVINLVSFYFLSARAPLISLLIFVLT
                     FGLLLFLISFFRIPKRQLTIQDNAIIAPADGKVVVIEEVQADEYFTDRRIQVSIFMSP
                     LNVHVNRNPVSGDVAYSQYHKGKYLVAWHPKSSTENERHTVVYRKDGKEILVKQIAGA
                     LAKRIVNYLSVGQQVKQTDEMGFIKLGSRVDLLLPLDAKIHVKIGDKPQGGVTVVATW
                     "
     assembly_gap    5906..6005
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6006..6242)
                     /locus_tag="PLO70_04940"
     CDS             complement(<6006..6242)
                     /locus_tag="PLO70_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HQZ73835.1"
                     /translation="MQYKHLFFDLDHTLWDFEANSHQTLKELYLLLNLQEKGIEDFDL
                     FHKNYLAHNDKLWDRYRNGYIKVDELRWKRMWLSL"
     gene            6325..7023
                     /locus_tag="PLO70_04945"
     CDS             6325..7023
                     /locus_tag="PLO70_04945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ73836.1"
                     /translation="MNVIITGASKGIGKAIAEIFAANGYDLFLCSRGEAALYKTMEEL
                     VTRFPAVTIKARPFDLSKKEDAVAFGNWCLSYDVPDILVNNAGLFEPGSVYNEPDGQL
                     ESQMATNLYSAYHLTRTILPKMMERKTGYVFNICSIASLHAYTNGGAYSISKFAMYGF
                     NKNLREEMKPHGIKVTAVHPGAVLTDSWGEYDNSAKRIMEASDIAKMVYASSQLSMQA
                     CVEDIVIRPQLGDL"
     gene            7103..8890
                     /locus_tag="PLO70_04950"
     CDS             7103..8890
                     /locus_tag="PLO70_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQZ73837.1"
                     /translation="MKKVAFILSLIILSGNSNAQVLFKTIVPSEPVIAGESFRVQYFA
                     EDASAISNFLAPDFRPFRMVAGPDIYPGEMSHQNNFRQSKNYVFTLVATTPGKYIIPG
                     AVINNNGKQIKSNEVMIEIISKDNAQKKLNKEAAVRQSEYYLRPGEDVYQKIRQNLFI
                     KVVVDKKSCFVGQPVLATFKLFSRLESKSDIVKNPGFYGFTVYDMVSLADKQLTTEYV
                     NGKPFDVHMIRKVQLYPLQAGTFIIDPMEVKNKVEFSRSVVNKKTEQQIVEGMLNSSG
                     DTDLPAEGAEVVETEISTAPVSIEVKPVPEKNKSNDYTGAVGKFTIAAKLEKSDLKKN
                     EEGFLEIIISGNGNFTQLGSPSIQWPSGIEGFDPLVLDSVDKAKIPLAGKRKFRYGFV
                     CASHGKYVLPAISFSFFDPDSNKYKTISTKPLPVSVSNEEKKTIIIEEKKTSIAAQSE
                     KISRIAIGVVVFLVLSVLAYWTLRKREPAVPTVIREKTYASVEESLQSVRDLKTTSDK
                     ELFKELNEAIWKHLSIYFILAGTEKNAHRLLSILKEKGAGIAAISSLNEILSDCEAGM
                     YANASLSVDKEELLKRTKAVLEEIDKTLL"
     gene            complement(8882..9589)
                     /locus_tag="PLO70_04955"
     CDS             complement(8882..9589)
                     /locus_tag="PLO70_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73838.1"
                     /translation="MSADKKTSILLVEDEENLHEALKLNLELEGYEVTSAFDGAAALK
                     AVENEYFDLIILDVMLPEMDGISVTETIRISNNEVPILILSAKNSSADRVLGLKKGAD
                     DYLTKPFNLEELLLRVHKLINKNKKLLDKSTIGDTYTFGNITVDFKAQEATGFNGEKI
                     QLSKKETMLLKLLIENKNEVVPREKILQAVWGYNVYPTTRTIDNFILNFRKYFEEDSR
                     NPKYFHSVRGVGYKYSE"
     gene            complement(9586..10575)
                     /locus_tag="PLO70_04960"
     CDS             complement(9586..10575)
                     /locus_tag="PLO70_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ73839.1"
                     /translation="MPDESRKRLRRTTIIYWTLLLYIIAALVWWFISLEKQNQQIAKQ
                     RYINLSSQTDSLTTLRLAEKLDTINDETNRNTGKYIAEGITFLILILIGAAFVYRSVR
                     RQFRLQQQQQNFMMAVTHELKTPIAVARLNLETLQKYNLDPEKQKKLIRTTLDETTRL
                     NFLTNNILISSQLEGDRYKSDKEELDLSMLLKDCVQDFKKRFPDRQFAENIEADADVK
                     GDPLLLQILINNLLENAIKYSSKEGLIKAVLKKSDTGIKLQIIDEGHGIPDEEKKKVF
                     TKFYRIGNEATRKAQGTGLGLYLCQKIARDHNADILVTNNEPKGSNFAVIFKL"
     gene            complement(10591..11076)
                     /locus_tag="PLO70_04965"
     CDS             complement(10591..11076)
                     /locus_tag="PLO70_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HQZ73840.1"
                     /translation="MKKFLSILYSILLTSYIISSCTTIDLYEKSVTMPGHSWKGSYKP
                     SFTFTIKDTSSPYKIYFVLRHNDKYSFNNIYINLYAQQPGQDSVQSVRYDLRLATNEK
                     GWLASGMDDIYEHRILLTPNGQEFYFKKPGNYTFAIEQIMREDPLKNILNAGLRIEKK
                     Q"
     gene            complement(11131..12006)
                     /locus_tag="PLO70_04970"
     CDS             complement(11131..12006)
                     /locus_tag="PLO70_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQZ73841.1"
                     /translation="MLKSMTGFGRAEKNVGDKTFLVDIKSLNGKQFELQLKLPAFLKP
                     FEFDIRRLLSEKLGRGSVDCMISLKDTGSSKPVTINTDLAKAYYKPLAELSAALNLDT
                     SHILSTLVKLPEVITPSSETLTDKEWENFKDVLQLAIDNLNSHRLDEGKSLEEDLLIR
                     ITNILKQQDEVIKLEPLRQQKIREGITRLLEENVGKENYDGNRLEQELIYYIEKIDIT
                     EEQVRLKNHCEYFKTLLAESDESKGKKLSFILQEIGREINTTGSKAYDSTIQKCVVLM
                     KDELEKAKEQVLNVL"
     gene            12685..13641
                     /locus_tag="PLO70_04975"
     CDS             12685..13641
                     /locus_tag="PLO70_04975"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQZ73842.1"
                     /translation="MRFDNPVPVTTIAQLIGAELIGNIDGYVTGINELHKVEFGDLAF
                     VDHPKYYSTCINSAATFIIINTKTCFPEGKALLVVDDPFEAYQKIVSFYRPFTPSLKM
                     ISESASIGAGSVIMPGAYIGNHVSIGSNCIVHPNVTILDHCIIGDHVIIQAGSVIGSD
                     AFYYNKKTNREIHYKKMASCGRVLIENAVEIGANCTIDRGVSHDTIIGAGTKMDNLVH
                     IGHDTVIGKNCLFAGQVGIAGATTIEDNVILWGQVGVSKTLTIGKGAVVYAQSGVKDS
                     IEGGKVYFGSPVEEAREKMKEFVWIKRIPQLWEKVMGSKDKG"
     gene            complement(13675..14796)
                     /locus_tag="PLO70_04980"
     CDS             complement(13675..14796)
                     /locus_tag="PLO70_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ73843.1"
                     /translation="MQNLRFVFISLLSFFTITATAQFVVTGTVLDSATREPLSNASVF
                     CQNTTLGTVTQKNGEFSLSLKSGGYDLIFSYTGYQTQTIRISDNNKLEILLVKEDKSL
                     GEVVLKSSFEVADGWEKYGDFFIKNFIGSTPNASKCVLTNPEVLKFFFYKKTNRLKVM
                     ATDALLISNNALGYQLRYQLDSFVYFYNTDINLYRGYCLFSEMEGTDSLKNLWSKARV
                     NTYEGSRLHFMHSYYDSTLLEEGWIVDLLDENNDKKFIKVKNVYDTAYYNYVQNMTDS
                     IGEDSVVHTITTGPAEVEIYYPRKISITYTKKSPEKQYLAKMKLPKSVPYQISYIDIK
                     NWITITENGYYYEQKDWINQGYWSWKNLADLLPYDYLPD"
     gene            complement(14835..15839)
                     /locus_tag="PLO70_04985"
     CDS             complement(14835..15839)
                     /locus_tag="PLO70_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ73844.1"
                     /translation="MPAKILITGGTGFLGSYIIKQLVEKNYAVRAIRRGNKLPTWISK
                     EITDKVEWVEGDILDVVALQDAMESVDTVIHSAAIVSFLRKDREQMYQVNVEGTANVV
                     NMALEKNVSRLVHISSVAALGRTAGGGHVNEEKKWEESKVNTHYAKSKFKAELHVWRG
                     VSEGLDAVILNPSTILGFGDWHSSSCAIFKQVYEGFNWYTPGINGFVDVEDVAKAVIH
                     CMESDISEQRFIINGDTWPFKKLQDTIADNFKKKRPGRKTTPFLLAIAWRLEKVKSLL
                     TGKKPLLTKESARVAQSQTFFENDKILKALPGFSFTPLEETIENACKKYLGTINAVQP
                     "
     gene            complement(15911..16759)
                     /locus_tag="PLO70_04990"
     CDS             complement(15911..16759)
                     /locus_tag="PLO70_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQZ73845.1"
                     /translation="MNINSICVCGAGTMGSGIAQAAAQAGFATILYELNTTILEKARA
                     GIENSLLTLTAKGKINAAEKERIFKRIHFTDDIQTCLADVFIEAIIEKPSAKIALFNQ
                     LAELNHSECIFASNTSSLSITHIAEKIQHPERVIGMHFFNPATIMKLVEIVNTNFTNR
                     QTTETIIELSKQMGKTPVLCKDSPGFIVNRVARPYYIESLRLAEEGVSEFAQIDLLME
                     ATGFKMGPFRLMDLIGNDVNYAVSCSVYEQMNNPERLKPSYIQDEKVREGKLGKKTRE
                     GYYKYN"
     gene            16851..17429
                     /locus_tag="PLO70_04995"
     CDS             16851..17429
                     /locus_tag="PLO70_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73846.1"
                     /translation="MDTNQPASSASYTPAPAAPGLFGTKIPSSVSFAVGVLLFFMPFL
                     DIKCNNMSLQKISGIELATGFHIKSPGGNNSLMGNFENSTGNKAESKGDKKDPNMFAL
                     AALVLGIGGFVVSLLNHRAGGFGGMLTGALSAAAMIGLMMDIKSDIKKELLGGKDGVT
                     IAVEFTPWFYIAIIAFLAAAFFCYKRIQSTKN"
     gene            17500..18402
                     /locus_tag="PLO70_05000"
     CDS             17500..18402
                     /locus_tag="PLO70_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73847.1"
                     /translation="MRACIAILFSCFFLAGYGQTFERWAQVVNWDGFSHWSRYMITQP
                     GFQGPNSLPVPRIGNGSADSSFAVGVTGNLHFSNGDNTQNLSVFANCSVVKELVSIDL
                     MWVPYEHYTMSTAIKEKRHVFSPYYNDHSTPGDIHLNTTFQLLKKWRESIQLALRIGF
                     RFPSGSDFGAARYTDGPGYYFDISMGKPIKNSSVKWIAMMGFYVWQIKSDHHNQNDAL
                     LVGSGLEWNKKSLRLQTYIAGYMGYLQNIGDDPFVFRANMEKKLHRTTFLLGFQQGLK
                     DFAYSSVEVGAKYNFRWKNNMLAK"
     gene            complement(18395..19423)
                     /gene="pheS"
                     /locus_tag="PLO70_05005"
     CDS             complement(18395..19423)
                     /gene="pheS"
                     /locus_tag="PLO70_05005"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQZ73848.1"
                     /translation="MEQLLKQIEGYKKDITSFSATDDKQVEEFRIKYLGTKGIVKAIM
                     SEMKNVAPEKKKEAGQLLNEFKQCVEARFAELKEATSNTEHQTSNIDLSLPGDELPLG
                     SRHPVTLMRNRIVSIFQRLGFAVAEGPEIEDDWHNFGALNLPEHHPARDMQDTFYIQQ
                     NPDWVLRTHTSSVQIREMEKGKLPIRMLMPGRVYRNETVSARSHCFFHQVEGLYIDEN
                     VSFADLKQTLYFFVKEMYGKEVKVRFRPSYFPFTEPSAEMDVTCFICGGGGCNICKKT
                     GWVEILGCGMVHPNVLSNCGIDPNKYTGFAFGMGIERPAMLKYGVNDLRLFSENDVRF
                     LRQFTSAI"
     gene            19540..20676
                     /locus_tag="PLO70_05010"
     CDS             19540..20676
                     /locus_tag="PLO70_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQZ73849.1"
                     /translation="MSDHLNISDFLSPVSLSAISHDEGYKDGQLGKVIAVYDDEFPDL
                     DEVQLVIVGCGEQRGSGLIHGHSEAPDKVRRHFYNLYYWHQDIKIADVGNIKAGSLFT
                     DSYAALKTVVQELMGDGKTVIILGGSHDLTLSQYYAYAETKRTIEASCVDAVIDLNLD
                     SPFRHENFLMEMLTGEPNFLRHYNHIGFQSYYVHPRMLETLDKLRFDCYRVGSVKESI
                     DEMEPVIRNSNMFSFDITAIANTYAPANTISPNGLNGEEACVLMRYAGMSPNINSIGV
                     YGYDAQHDKHELTAKQISHMLWYVLDGRSRGKREAALDEKDSFNEYHMAFAEVDTIFL
                     QSKKTGRWWMQLPDKKFIACSYKDYLMASSNEIPERWLRAQERG"
     gene            20734..21780
                     /locus_tag="PLO70_05015"
     CDS             20734..21780
                     /locus_tag="PLO70_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ73850.1"
                     /translation="MASFIRTGICSYGMSGKLFHAPFIQAHPGYELSAIVERHNHDSK
                     ERYPDAKLYRSVEELCADTDLQLIVVNTPTHLHFEQAKIVLQAGKHLVIEKPFAVTVK
                     EAEELSALAVKNKLFISVYQNRRYDGDYRAVKNVIEQDLLGELREAEIRYDRYRPTFG
                     GKQHKEGDMPGAGILFDLSPHLVDQAIQLFGFPKAVFADIWKMREDVIAPDYFEILFY
                     YGKLRVRLKATCIARETTYAYTLHGMKGSFLQHRSDMQEQQLQSGVKPSLENWCPAPA
                     KPDGLLHTEINGEVIRKETTSSPGNYMGYYDDVYKALTGQGPNPVPAADGIKNMRIIE
                     AALESFASGKIVSL"
     gene            21791..23176
                     /gene="dacB"
                     /locus_tag="PLO70_05020"
     CDS             21791..23176
                     /gene="dacB"
                     /locus_tag="PLO70_05020"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HQZ73851.1"
                     /translation="MSYEFSITGKKRLFKCIKQFIILNSSFIILLSSCSIEKQISKSA
                     RADVLNTKALQAAHVGISIFDPSANKYLYNYQADKYFVPASNTKIPTCYAAMKYLGDS
                     LVAALVLENDTAILITPTGDPTLLHPEFKIQPLINLLKKEKRQIYINDDNWQDEALGS
                     GWSWNDYDQSYMAERSPLPVYGNILEWTQEVNETNKPPLVYSSPETNWEVSFSDNEKA
                     RTFSVTRSIDKNYYTITLGVEKKRTITIPFVTNRVQSALELIKDTIFKEINKIELNKI
                     LIPGHGFTNSLKKINSQPTDSLLKPMMHRSDNFFAEQSLLMVSNERLGVMNDAKLIDT
                     ILKSDFKDLPQKPRWVDGSGLSRYNLFTPQDFIAILNKMKNEFGMDRIKVIFPTGGEG
                     TISSYYKKDNGYIYAKTGTLSGVVAFSGFLYTKKGKLLIFSTLVNNHQASATDVRRAI
                     EKFLQRVRNKY"
     gene            complement(23244..25097)
                     /gene="mutL"
                     /locus_tag="PLO70_05025"
     CDS             complement(23244..25097)
                     /gene="mutL"
                     /locus_tag="PLO70_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQZ73852.1"
                     /translation="MAAKADFVPDKITLLPDNIANQIAAGEVIQRPASAVKELLENAV
                     DAGATEIKLIIADAGKSLIQVIDNGSGMSETDARMCFERHATSKIKNIEDLFHIRTMG
                     FRGEALASIAAVAQVELKSKRAEDETGVYIEVENSVVKKQEPIAAPQGTSIAMKNLFF
                     NVPARRNFLKSNAAEMRHIVDEFTRVAMSFPEMLFTLTANNQQLFHLEAGSLKQRIVQ
                     LLGNNYNAKLVTVKEDTDYMNIYGFVGKPETAKKTRGDQYFFVNNRFIKSPYLNHAVM
                     NAYQEMISSDNFPMYVLFIDLDPTFVDVNVHPTKQEIKFEDEKIIYAFVQAAVKHALA
                     QFSITPTLDFDLDASIQQLSSIQQPFTEEKKSAAASGSIFKGFTQKNQAHFIEKSERS
                     ELKHWKDFYEGGQQSEAGSQVPESSASSLSDFKQHTSDFNELTQLLNTYITVPSKNGF
                     LLIHQQSAHERVLYDQLKTASKDKPVATQRSMFPSTLELTPADAAVMEEIITDLQQLG
                     YSIEPFGKNTYVIQGTPADVEAGNEKHIIDILLEQYKHFSNEVKFSKREKLIRSLARQ
                     QSIKTGTRLTEREMRQLVNDLFACEQSNVTPDGNPTYLEFKQEQLEGMFGK"
     gene            25194..>26431
                     /locus_tag="PLO70_05030"
     CDS             25194..>26431
                     /locus_tag="PLO70_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQZ73853.1"
                     /translation="MTILRAALLCLFFIPFVATAQYKSKLNREQWVDSVFNSLSDDQR
                     IAQLMVIRAHSNLGPDHVAKVVNDIRQYNVGALCFFQGGPIRQANLTNYYQSIAKTPL
                     MVTIDGEWGLGMRLDSVTRYPYQLTMGAINDENLVYRMGVAIGEQCKRIGVHVNYAPV
                     IDINNNPNNPVIGYRSFGEDKDKVSKYGVAIMKGMQDAGIMACAKHFPGHGDVDVDSH
                     YDLPVINKSMKQLDSMELVPFRAIFNAGVGSVMIAHLYIPSIDNTANKATSISKNNVT
                     DLLRTKMGYEGLTFTDALEMKGVAKYFPGGTIAVEAIIAGNDMLCLPASVPESIETIK
                     QAITDNRLSWYDIYAKVKKVLASKYQLGLNKWSPIDTNNLLADINAKTDAIRYEVAQH
                     SITILSQAGTKASRTDYAQVP"
     assembly_gap    26432..26531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26532..27959
                     /locus_tag="PLO70_05035"
     CDS             <26532..27959
                     /locus_tag="PLO70_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQZ73854.1"
                     /translation="YKDLNFIKTITLTFGNVLATKNFCDAWTLVACYQDDAITHQAAA
                     DLLQGKIAAQGKLPVSVCSFKFGEGIMQGSTQPSKALIKERLQVVDSIALDGIAKKAF
                     PGCVVLAAHKGEIIYHKAFGNYEYDPASPVMSAESIFDLASVTKVSATAVSVMKLYEE
                     GKIKLDGKLKDYLPWVKGTNKEDLRIDDILLHQAGLVPFIAFYKETIDAATGTPNPAI
                     YAAKPQKGYGVRVAENIYLRDDWKDTMMMRILKSPVGPKNKYVYSDNDFIFLGKIIEE
                     ITGMTLDQYTQKTFYNKIGMYSTGFKPRNRFQLECLVPTETEKHFRQQTTRGDVHDEG
                     ASMFGGVAGHAGLFSNAYDLSLLYQMLLNGGELNGERYLKPETIKLFTSYNSKISRRG
                     LGFDKPEKDNAIRKDPYPSLLASPETFGHTGFTGTCVWVDPKQDLVYIFLSNRVTPTR
                     DNNKLSQLLIRGKIQDAIYRALGVE"
     gene            complement(27975..28187)
                     /locus_tag="PLO70_05040"
     CDS             complement(27975..28187)
                     /locus_tag="PLO70_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ73855.1"
                     /translation="MNVPERLRQLRKKKGLTQQEIAEQLHMSQNAYSLLESGKTKLDI
                     QRLHLLAQFYKLSIYDIIDYLPPEKK"
     gene            28374..29027
                     /locus_tag="PLO70_05045"
     CDS             28374..29027
                     /locus_tag="PLO70_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73856.1"
                     /translation="MKKTCLLLGIVLVLFNLSCDKEEKGQKVDVKRLTVGNILRFGSV
                     NGNSSELFAPIEIGELPGGGSCNCNGHGGSSGGILSWTLATCRSNCTRGIGFRCGREG
                     ILFCQDGTTIICTWGSNCPSQAKGTVPERDMEASYMFYDNGTMKLTFNKQIPDLEKNA
                     ANGDIFEVESTDYVPFSSAVSVDGVYYTGFDFLQGNYHIDYTDGQYGSVVIGIQLKQ"
     gene            29074..29766
                     /locus_tag="PLO70_05050"
     CDS             29074..29766
                     /locus_tag="PLO70_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73857.1"
                     /translation="MKFILSAIIVLYCSGCSVVARKGYGAKKVKIENEQTIQNWLSEK
                     GFSTRNVVTVTPESYYDNYFGLPQAPLLFDKASGNFLAIGFTNGEYCPKDVDKSFSSI
                     PHYSLMKVKPDSFLISETTSIPPGFSIKDKDKFEKKIDTLPIKLTQITSYFRTLKGEP
                     SPEFLQKKSDYILIIPFAIFLGKKLQVKDLKKYYYSALTNRSAMIQIVFLNLDKQEWW
                     GEEWNKKINITY"
     gene            complement(29756..31150)
                     /gene="ricT"
                     /locus_tag="PLO70_05055"
     CDS             complement(29756..31150)
                     /gene="ricT"
                     /locus_tag="PLO70_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQZ73858.1"
                     /translation="MSCTSCSTASNGKPGGCKSNGGCSTGGCNRMNTHDWLRNLPISD
                     VASACKVIEVSFSQGTRKDFYRNPTLQPYEKGDLIAVEGVSGFDVGEVSLTGEIVRLQ
                     MKKRNVKEDNPEMKKVLRVATDRDIDIWKQNKAREPEAVIRSRAIAKQLKLDMKISQV
                     EMQADGRKATFFYIADGRVDFRELIKVYASEFKVKVEMRQIGARQEAGKVGGIGSCGR
                     ELCCSTWLTDFSSVNTTAARYQNLSINQTKLSGQCGRLKCCLNYELDTYLDALQHFPD
                     NCDVIQVAKGNAFLIKKDIFKNLMWYTLPDSNKQYPLTIERVQKIKSLNQQGIKPDDL
                     EPVEVTSGKPKEAEPEFVELVGQISLKSLDKAADKKRRQNQQQQRGQRPQQGGQQRQQ
                     GNQQGGQQGGQQNQQRRQQGGQQRQQGGNQQRPNNPNRNQRNQPDRQSGQQSGNQQRP
                     NNPNQGKDKPSTNT"
     gene            complement(31383..32594)
                     /locus_tag="PLO70_05060"
     CDS             complement(31383..32594)
                     /locus_tag="PLO70_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73859.1"
                     /translation="MQFADIIGQQEVKQQLAELVQHNRLSHALLFLGKEGSGSLSLAL
                     AFSQYVVCEKVNGKNTSIQAGPSLFGDPQPQTSNLKLQTDSCGVCSACLKAQQFVHPD
                     IHFSYPTVTKKPGEKPIATDFIAEWREFLKLNPYGNSFDWIELIKEKENSQGKITAEE
                     CNDIIRKLSLKSFESEYKILIMWMPEMLGTEGNKLLKLIEEPPPNTLFILVTENEAQV
                     LQTIVSRCQLVKIPALATRDIEEALINRNKTEPAIARQVAGVSDGNYREALQLVQHAE
                     EDWQSLLREWLNAILKTGPVAQTKWVEEASRLGREKQKQFLRYFNHLLEQAIHYRIMG
                     ERLNIGEKERDFAERLNKIAGIEQQQAIIEELDRASYYIERNAHGKMLFHALTIKLYH
                     IIQDKTVFLVN"
     gene            complement(32763..33281)
                     /locus_tag="PLO70_05065"
     CDS             complement(32763..33281)
                     /locus_tag="PLO70_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQZ73860.1"
                     /translation="MKTLILSMVLAATLTDGSIYDFKIAGLDGKDIDFAQYKGKKIMI
                     VNTASECGLTPQYEGLEQLYKKYKDKLVIIGFPANNFGGQEPGSNGEIKEFCKKNYGV
                     TFPMAEKISVKGNDIHELYKYLQAQAAAKGFADPVTWNFGKFLVNEKGELIATFSPRT
                     VPMSPDILKYLN"
     gene            complement(33348..>33603)
                     /locus_tag="PLO70_05070"
     CDS             complement(33348..>33603)
                     /locus_tag="PLO70_05070"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HQZ73861.1"
                     /translation="IFNVEFDHNLWKVQTIPSNITSNQIINNQSPQFDSINTSRNYYD
                     FRLATSSPAINKGVNTTVITDLDGKTRPVGLPDLGCFEKQ"
BASE COUNT         9519 a         6408 c         7019 g        10457 t
ORIGIN      
        1 taccgggaaa aatgtacaaa agcactcgaa tgatacagtt aggccgcctg ttccatttta
       61 ttccttaaag ggtgagtcag gcaacggaga taccattgac tttaattcct tccagggtaa
      121 aaaagtgtta cttgtcaata cggcaagtaa ttgcgggtat accaatcaat atgatgattt
      181 acagcaacta tcagaacagt ataagggtaa attattggtg atcgggttcc ctgccaatga
      241 ctttaaagaa caggaaaaag gtaccgatga agagataatg cagttttgca agcttaatta
      301 tggagtgact ttccccctca tgaaaaaaag tgtcgtaatc agatcggcgg gacaaaaccc
      361 ggtttttcaa tggctgacgg attcagcaaa aaacgggtgg aacaataaat atccgtcatg
      421 gaatttttcc aaatacctgg tgaatgaaaa tggaatactg actaattatt ttgatccttc
      481 tgtttcaccc ctgggcaaag agatcaggga agccattgaa aaaaaatagt ataccatatg
      541 tttacgaagt tagatataga agcatacttt accggggaga agcaagaaag tcttttgttt
      601 ctgtttattg gtattgcggg tatcagtaca gccatcattt tctttttctt gttgaaaact
      661 agtttttacc tgggagctgc tattccttta ttagtaatcg ggctgttact ggggatagtg
      721 ggttacacca tatataaaag gagcgacgat gaccgtaaac ggaatgtgta tgcttacgat
      781 atgaatcctt cagcgcttaa agaaaaggaa ttgccgagaa tgaaaaaggt aatgaagaat
      841 tttgttattt accgatggat tgagatcttt ttgttcttgc tgggtactgg tctttatatc
      901 tgtttcatcc gtgattttaa acatgacttc tggcggggtt ttggacttgc attggctgtt
      961 atggcccttt tagccttagc tgccgattat tttgcagaga aaagaggaag catctacctg
     1021 aaagggatcg aatcatttgt acaatagctg ttacactaaa ccttctttcg gttattatat
     1081 tggccaatca gaatagcagg ataaataaat ttatcattta atcaaaacta tgaccgtcgc
     1141 agatatcatt gggacaattg gtgtgggatt aatcttaata gcttacttct gcaatacctt
     1201 cggatggata agtggaaaag gaaaactttt ctttttattg aatgcagcag gagccgggct
     1261 ggcctgttat gcatcatggc tgatcagcta ctggcccttt gtcgtactcg aaggaacatg
     1321 gttccttatc tctgttgtag ggctggcaag aacgtttaag gttaaagatt aattcaccgg
     1381 gcatttctcg tggtagttca ccagctcaat tggtgttttc tcaaactcat atcctttgta
     1441 agtactggca atgttttcaa atacatcgtt taattcttct tcggttttta aagtcaccag
     1501 tttattgcgg aactctttaa tatttggtaa gccctttaaa taatttgcat agtgacggcg
     1561 catctcatta atacctacca caggattttt ccattcaatg gactttcgta aatgtttgcg
     1621 gcaaacaaac aaacggtctt caattgtagg agctggcaag tgttccccgg ttttcatgaa
     1681 atgctttatt tcattaaaga tccaggggta gccaatggca gcacggccga tcatgatgcc
     1741 atctacgcca tagcggttct tatattccag tgctttttca ggggaatcaa tatcgccatt
     1801 accaaagatt ggtattttga tcctgggatt gttttttact ttaccgatca gggtccagtc
     1861 agcttcaccc ttgtacatct gcgttctggt gcgcccatga atagctaatg ctttgatgcc
     1921 ggcatcctgc aaacgttcag ctacttcttc aatatttctg ttgttatcat cccagcctaa
     1981 tcttgttttt acagtaacag gtaaggaggt tgattttaca acagcggatg tcagtttcac
     2041 catcaggtca atatctttca gtactcctgc accggcgcct ttcaaagcta ctttttttac
     2101 cgggcagcca aaattgatgt ccaataaatc aggattggta acttctacaa tctttgctgc
     2161 taaagccagg ctatcttcat caccgccgaa tatctggatg ccgaccggcc tttcataatc
     2221 gaaaatgtca agtttcttac gacttttaat ggcatcccgg atcaatcctt cactggagat
     2281 gaattcagta tacatcagat cggcgccatt gtccttacaa acagcacgga aagggggatc
     2341 gcttacgtct tccatcggtg ccagaagcag cggaaattca ggtagttgta tgttgccgat
     2401 tgtaaccatt ccgtttgtat ttgcctattt ttgtccttct gtttatcagc gataagcctg
     2461 caaatttaca atcaaaaaac cagagtagta catgaaggga ttattaaaga caaagtcagc
     2521 aggtaaccaa tttttattac tggttagtat ttcgttggtt agctttttcc tggtaggtct
     2581 tatcgggaca gtaatacttt ctgcattaac aggcatggat ataaaagaaa tgagtgatat
     2641 gaccaagtgg gattacagca aacctgctac cattaatttt atccggggta tgcagctcat
     2701 ccagtttatc agtctgtttt taataccaac atttatttgc gccaggctat tcagtacgga
     2761 taccaggcaa tacctcgggt taagaaaacc ttttacccgt tcttattggg taataggaat
     2821 attggtaatg atactggcga taccttttgt acaatggctc ggtgaactga accggggtat
     2881 ccgtttccct tccgggattg aacactggat gaaggccaaa gaagcggagg caaacaaaac
     2941 agtaatggct ttgctatcca gacaaacgat caaagatctt attctgaata tcatttgcat
     3001 agcaggatta gctgcggtgg gtgaagaatt attgtttcgt ggtatggcac agcgtctgct
     3061 gataaaaatg ttcaaaagcc cctggcccgg aatattgatc gccgcctttc ttttttcagc
     3121 gatgcacctg cagttttatg gattttttcc aagatttatt ttgggcatat tattaggaat
     3181 tgtatactgg tacagcggca gtttatggcc ggcgatcctt gctcattttg tgtatgatgc
     3241 tttattaatt acactggcat atttcaaccc gtcaatgctg aatgaagaag ctacggtgaa
     3301 gctttcttct attgcattaa cggcagcgat tagttttatt atgctggccc tggcggtaaa
     3361 ctggatgatg aaaaattcaa aagcagatta taaccaggta tatgcagaag atgaagtacc
     3421 tgtaaaagat catcctttct gaattaactt tataatatgg catttaactg gcaaactttt
     3481 aaaacaaggg cattaactgc agttgtgttt gtagtagtaa tgctggctgg tttgctctgg
     3541 aaccactgga gttttttcat tctattttcc attattcatt ttggttgttg gactgaatac
     3601 cagaaactgg tgggattgat cgatcccggt tataaagaga taacaccttt tcataaatat
     3661 ggagtgatgc tggccggctg gtgtttgatg ttgtggttta gcggagatga cctggcaatt
     3721 ggcggtttac ggcttcatga aatgggatgg tggattggtt tggttctggt gtttcttttg
     3781 cccatcacag aattattatt ctccaaaaat atacgactta aaaatatagc ccattctgtt
     3841 ttcggtttaa tatacatttc acttagctgg gggttgatga tggatatgta tgatcacccg
     3901 gcagctgttc atatccacaa tacaaccttt gtaattgaat ccggaatttt tccaatcata
     3961 atcattataa gtatctggat aaatgataca atggcttata tagtcggctc tttaatcgga
     4021 aagaccccct tatctccgat atcaccaaaa aagacatggg aaggaacaat tggtggtatt
     4081 ttactggcaa ttgctgtagt tggcttttta tttcctttct tttatttcgg ttcatctgct
     4141 tttgcacttt ttatgtttat tgcacccatc gctgctatag cagctattgt cggaacatat
     4201 ggtgatctgc tggaatcaaa actaaaacga ctggcgggtg ttaaagacag tggccagatt
     4261 atgccgggac atggtggctt tttagaccgt tttgattcct tgttactggc aacacctttt
     4321 gtgtggttgt atgtgcagtt gtttatgaaa tgatcagtta aatatcaggt cataaaaaat
     4381 atatgggtaa attgatctgt agtttatctc tactttttat tacctcttct gcaggagcgc
     4441 aaagaatttt ttcggatgga aattggggtg ctacacttgc aggtgcaatg gttccgctgc
     4501 ctacttttac tcttggtgta cagcccgggg ttttatataa gatttccgac cgggtatctt
     4561 tattaacaga actgaccatt cctgtagtaa agaatattgg tgataattcc ggaacaacaa
     4621 atcaaaagta tttcagaatt caaccggagt tcaggtatca ttttccattc aatatgagga
     4681 gaaacaatat ttatatgggt ttgcggctgt cttatgcaat ccgtaaattc gaagatgcgg
     4741 atggaggctt ttattccgat gtaactccag gttttgacga gggttattat tttgataagg
     4801 cacaagttaa tagcccaata atgacatctt ctttgcaggt tggaactata acaaatggaa
     4861 ataagaaaat aaccaccgat gtgttttggg gatttggtgt aagatatata aacactgagt
     4921 atacagatgt agttaatccg gtaagtggta tcaggaatag accggcagac gggccactat
     4981 tttatccatc ttattcatat aacagagcag ttacctggct tcatgtgaat ggagggttcc
     5041 gcttgattta tcattttgga agataaggta atgataatcc cggtttgctt atttttgcca
     5101 ttcaataagg ctcaatgaca atccacaaag aaggctataa gaccattgcg atcagtacta
     5161 ttgtttttgg ggttatcaac ctcgtttctt tttattttct gagtgccaga gcaccgctga
     5221 tcagtttgct gatatttgtc ttaaccttcg ggttactgct ttttctgatt tcttttttcc
     5281 gtatacccaa aaggcaattg acgatacagg ataatgccat cattgcccct gcagatggta
     5341 aagtggtagt gatagaagaa gtgcaggccg atgaatattt caccgaccgg cgtatacagg
     5401 tctccatctt catgagcccg ctgaatgtac atgtaaacag gaacccggta agtggtgatg
     5461 tagcctatag ccagtatcac aaaggaaaat acctggtggc ctggcatcct aagtcttcta
     5521 ccgaaaatga aagacataca gtagtgtacc gtaaagacgg taaagaaatt ctggtaaaac
     5581 aaatagccgg tgctcttgcc aaaagaatag taaactatct ttcagttggt cagcaggtta
     5641 aacaaactga cgaaatggga tttattaaat taggctcaag agttgattta ttgttgcccc
     5701 ttgatgcaaa aatccatgtc aagattggtg ataaacctca gggcggtgta acagtcgttg
     5761 ccacctggta gtatcaattt ttttcagaat atatcttcta actccctcag cgaataaaca
     5821 gtgtaagtag ggaattgatc attaattacc ggcactgctt cttttgtcag atggttaaca
     5881 tgtacctggt caataccagc atttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnncagtg ataaccacat ccttttccac ctcagctcat ctacttttat gtagccatta
     6061 cggtaacggt cccatagttt atcattatga gccaggtaat tcttgtggaa aagatcaaaa
     6121 tcttcaatgc ccttttcctg aaggtttaat aacaggtata attctttgag cgtttggtgg
     6181 ctgttggctt caaaatccca tagggtatga tcaagatcaa aaaacaggtg tttgtattgc
     6241 ataatcgtaa agtacgagct gcgaagttag gcgtatggtt tgaaccggaa aaagttcgta
     6301 cttcgtactt caaacgctac aactatgaat gtgatcatta ccggggcatc gaaaggaata
     6361 gggaaagcca ttgcagaaat atttgcggct aacgggtatg atctgttttt atgctcccgt
     6421 ggagaagcag cactatataa aacgatggaa gaactggtga cgaggtttcc ggcggtaacg
     6481 attaaagcca ggccttttga tttgagcaaa aaagaagatg cagtagcttt tggtaactgg
     6541 tgtctttcgt atgatgtacc cgatattttg gtaaataacg caggcttgtt tgaaccgggc
     6601 agcgtttata acgaaccgga tggacagctg gaaagtcaaa tggctacaaa tctttacagc
     6661 gcttaccatc tcaccaggac tattttaccc aaaatgatgg aacgaaaaac cgggtatgtt
     6721 tttaatattt gttccattgc ttcattacat gcgtatacaa atggcggtgc ctatagcatc
     6781 agtaagtttg ccatgtatgg gttcaacaaa aacctgcggg aagagatgaa gccgcatgga
     6841 ataaaagtaa ctgctgttca tcccggtgca gtgctgaccg attcctgggg agaatacgat
     6901 aattctgcca aacgtatcat ggaagccagt gacattgcta agatggtgta tgcgtcttct
     6961 caactctcca tgcaggcctg tgtggaagat attgtaatca ggccccaatt gggtgatctc
     7021 tgaccattga aaagctgatt ttcccattaa taatttttta attcctttta agaaggcagg
     7081 gattattttt aaagctgctt aaatgaaaaa ggtggccttc atattatctt taatcatact
     7141 gtctggcaat agtaatgcac aggtattatt caaaacaatt gtgccatctg aacctgtaat
     7201 tgcgggtgaa tctttccggg ttcagtattt tgcagaagac gcctctgcca tcagtaattt
     7261 cttagctcct gattttcgtc ctttcagaat ggtagctggt ccggatattt atccgggtga
     7321 aatgtcccat caaaataatt tcaggcagtc aaaaaattat gtgtttacat tggtagccac
     7381 cactccgggg aaatatatca tccccggtgc tgttatcaat aacaatggca agcagattaa
     7441 gagcaatgag gtaatgattg aaattatctc aaaggataat gcgcaaaaga aattaaataa
     7501 ggaagcggcg gtccggcaat ctgagtatta tttacgacca ggagaagatg tttaccagaa
     7561 gatcaggcaa aatctcttta taaaagtggt ggtcgacaaa aagagctgct ttgttggaca
     7621 accggtactt gctacgttca aattgttttc ccggcttgaa tcaaaatctg atattgtaaa
     7681 aaatcccggc ttttatggct ttactgttta cgatatggta agcctggctg ataagcagtt
     7741 aacaacagaa tatgttaatg gcaagccctt tgatgtacat atgatcagga aagtgcaatt
     7801 atatcctttg caggcgggca catttattat tgatccgatg gaagtcaaaa ataaggtgga
     7861 gttttccagg agtgtggtaa acaaaaagac agagcagcaa atagtagaag gtatgttgaa
     7921 tagcagcggg gatactgatt tacctgcaga aggagccgaa gttgtagaaa ccgaaataag
     7981 cacagcacct gttagcatcg aagtaaaacc ggtgccagaa aagaataagt caaatgatta
     8041 taccggagca gtaggtaaat tcactattgc agccaaactg gaaaaaagcg acttgaaaaa
     8101 aaatgaagaa gggtttctgg agattattat ttcagggaat ggaaatttta ctcagttggg
     8161 gtcgccttct atccaatggc cttcgggcat agaagggttt gacccgttag tcttggattc
     8221 agttgataaa gcaaagatac cgctggctgg taaaaggaaa ttcaggtatg gttttgtttg
     8281 tgccagtcac ggtaaatatg tgttacctgc cataagtttt tctttttttg atccggatag
     8341 taataagtat aagaccattt caacaaaacc gcttccggtt tccgtaagca atgaagaaaa
     8401 gaaaactata attattgagg aaaagaaaac gtcgatagca gcgcaaagtg aaaaaatcag
     8461 caggattgca ataggggttg tagttttttt ggtattatct gtcctggcct attggacact
     8521 gaggaaaagg gagcctgctg taccaacagt gatccgggaa aaaacttatg cttccgtgga
     8581 ggagtctttg caatcggtaa gggatttaaa gactaccagt gacaaagaat tgtttaagga
     8641 attaaatgaa gcaatatgga aacacctttc tatttatttt atattggcag gaactgaaaa
     8701 aaatgcacac cggcttcttt caattttaaa agagaaaggg gcaggtatag cagcaatcag
     8761 ctctcttaat gaaattttat cggattgtga ggcgggaatg tatgccaatg caagcctgtc
     8821 agttgataag gaggagctcc ttaaaagaac caaagctgtt ttagaagaga ttgataaaac
     8881 tttactctga gtatttatac ccaacacctc ttacagaatg aaagtatttg ggattacgac
     8941 tgtcctcttc aaagtactta cgaaagttaa ggatgaaatt atctatcgtt cttgtagtcg
     9001 ggtaaacatt ataaccccat acagcctgta atattttttc tctcggcact acttcatttt
     9061 tattttcaat aagcagcttc agcaacatcg tctctttttt acttaactga atcttttcgc
     9121 cgttaaaacc agtagcttcc tgcgctttaa aatcaaccgt aatattgcca aaagtatacg
     9181 tatccccgat agtggattta tccagcagtt ttttattttt attaatcagt ttatgaactc
     9241 taagcaaaag ctcttccagg ttgaaaggtt tggtcaggta gtcgtcagca ccttttttaa
     9301 gtcccagcac cctgtctgca ctgctgtttt tggcactgag tatcagaata ggaacttcat
     9361 tattactgat gcggatagtt tcggtaacgc tgatgccatc catttcgggg agcatgacat
     9421 caagaataat gaggtcgaaa tattcatttt caacagcttt taatgcagcc gcaccgtcaa
     9481 aggcagaagt tacttcgtag ccttccagtt caaggttcag cttcaatgcc tcgtgaaggt
     9541 tttcttcatc ttccaccaat aaaatagatg tctttttgtc tgcactcata atttaaaaat
     9601 aactgcgaaa ttactaccct tcggttcatt gtttgtcacc aaaatgtcgg cattatgatc
     9661 ccgggctatc ttctggcaaa ggtacagccc caacccggtt ccctgggctt tacgggtggc
     9721 ctcattacca atccggtaaa atttagtaaa taccttcttt ttctcttcat caggtattcc
     9781 atgtccttca tcaatgattt gaagttttat acctgtatca gattttttta gcactgcttt
     9841 tataagacct tcttttgagg aatatttgat cgcattttct aacaggttat tgattagtat
     9901 ctgcagcagg agcggatccc cttttacatc tgcgtctgct tcaatatttt ctgcaaattg
     9961 tctgtccggg aatcgttttt taaaatcctg cacacaatct ttcagtaaca tagaaaggtc
    10021 aagttcttct ttatccgatt tatacctgtc accttccagt tgggatgaaa taaggatatt
    10081 gttggtcaaa aaattcaatc ttgttgtctc gtccagtgtt gtacggatga gtttcttttg
    10141 tttttcgggg tcaaggttat atttctgcag tgtttccagg tttagccttg ccactgcaat
    10201 aggggttttc aattcatgtg ttactgccat catgaaattt tgctgttgct gctgtagtct
    10261 gaattgtctt cttactgacc ggtaaacaaa agcagcacca ataaggatca ggattaaaaa
    10321 agtaataccc tccgcaatgt atttaccggt gttgcggtta gtttcatcat taatagtatc
    10381 caatttttcc gcaagccgga gggttgtcag ggaatctgtt tgtgaagaaa gattgatata
    10441 gcgctgtttg gctatctgct gattttgttt ttccagtgat ataaaccacc agaccaacgc
    10501 tgcaataatg tataataata atgtccagta aataatggtg gtgcgacgta atcttttgcg
    10561 gctctcatct ggcatactgc aaagatgggg ttattgtttc ttttcaatac gcaggcctgc
    10621 gtttaatata ttttttaacg gatcttcccg cattatctgc tcaattgcaa atgtataatt
    10681 accgggtttt ttaaaataaa attcctggcc gtttggtgtc agtagtatcc ggtgctcata
    10741 aatatcatcc ataccgctgg caagccatcc tttttcattg gtggccagtc ggaggtcata
    10801 tctcaccgat tgtacagaat cctgtcccgg ttgttgtgca tacaggttaa tataaatgtt
    10861 attgaagctg tatttatcat tatgccgtaa aacgaaatat atcttgtaag gggatgaagt
    10921 atccttaatg gtaaacgtaa aagagggttt atagctgcct ttccagctat ggccgggcat
    10981 ggtaactgat ttttcgtaca gatcgatggt ggtacacgaa gaaattatat acgacgtaag
    11041 aagtatgctg tataatatag agagaaactt tttcaaacga atttttttac aaaaataggt
    11101 tacgaccgca tatgccgaac gcaatgcagg ctatagtaca tttaaaacct gctcttttgc
    11161 tttttccagt tcatctttca tcagaacgac acacttctgg atagtggaat cataagcttt
    11221 ggagccggta gtatttatct cacggccaat ttcctggagg ataaaggata gttttttccc
    11281 ttttgattca tcactttctg ccagcagggt tttaaaatac tcacaatggt ttttcaaacg
    11341 tacctgttct tcagtgatgt ctattttctc gatataataa ataagctcct gctccagccg
    11401 gttaccatcg taattttctt tgcctacgtt ttcttccagc aggcgggtaa tgccctcccg
    11461 gatcttttgc tggcggagtg gttccagctt tattacttca tcttgctgct tcaggatgtt
    11521 ggtaatgcgt ataagcaggt cttcttccag cgattttccc tcgtcaagcc tgtgggagtt
    11581 gaggttgtct atagccagtt gtaaaacgtc cttgaaattt tcccattcct tgtcagtgag
    11641 ggtttcgctg ctgggggtga tgacttccgg cagttttaca agggtactta atatatgaga
    11701 ggtatcaagg ttaagtgcgg cagacaattc tgccaaaggt ttatagtagg cttttgccag
    11761 gtctgtattt atagtaacag gtttggaact gccggtatct ttcaaactaa tcatacaatc
    11821 cacactgcca cggcccagtt tttctgaaag aaggcgacgg atatcaaatt caaagggttt
    11881 aagaaaagca ggtaatttca gttgcagttc aaattgtttt ccgttgagtg attttatgtc
    11941 aactaagaaa gtcttatcac ctacgttttt ttcggctctt ccaaaaccgg tcattgattt
    12001 aagcatcgaa aagtatttgt gtttgttgta aaggtatctt aattattggt tatactatgg
    12061 ctggttattt ttcctgtgga aggggattat ctttatgagt acttctgtaa ttttcgttcg
    12121 cggcagttgt attttctttc agtagtaacc tgggaaggaa caaaaaaatc cctccgtagt
    12181 aagcggaggg ataatatatg gatgggttag taagtacagg gaaataaatt tccctacaca
    12241 atattataaa ttattttctt tatccaaaaa atatttccaa aatattttat taacatttta
    12301 aaaactgctg tggataatcc ggacgttttt taagcctgtc tgtcaccata tttcatcgtt
    12361 gtaactttat ttttcattta ataattctat aaaatatttt tttgcttctt ttgaaacatt
    12421 gactggtaaa gtgtttcatc gcacaaggtt taattgtatt ttatgtagca acaatagttt
    12481 ttgtttttat taaagatgaa tcaatgtata cattcattca aaaaagagct atcatttccg
    12541 gtaaaaaaat attttacctt aatatcgttc aaaagctgct aaaagggtaa aatcactttt
    12601 tttaactgtt tagaaccagt tggcaggaca ttacctttgc aggagcagta aaaaaataca
    12661 gcatttaaaa tcaaaacaaa gtgtatgcga tttgataatc ctgttccggt aacaacgatt
    12721 gcccaactga taggagctga attgatcggc aacattgatg gttatgttac ggggataaat
    12781 gaactgcata aggtagagtt tggtgatctt gcttttgtgg atcatcccaa atactattca
    12841 acctgcatta attcagcagc tacattcatt atcattaata cgaagacttg ttttcctgaa
    12901 ggcaaggcat tattggttgt tgatgaccct tttgaagctt accagaagat cgtcagcttc
    12961 tatcgtcctt ttaccccctc tttgaaaatg ataagtgaat cggcaagcat tggtgccgga
    13021 tcagtgataa tgccgggggc ctatatcggc aatcatgtta gtatcggcag taattgtatt
    13081 gtccatccta atgtgacgat cctcgatcat tgtattattg gtgatcatgt tattatccag
    13141 gcgggttcag ttattggcag cgatgcgttt tattataaca aaaagacaaa ccgggaaatt
    13201 cactacaaaa aaatggccag ttgtggccgg gtgcttatag aaaacgctgt ggagataggt
    13261 gctaattgta ctattgaccg gggtgtaagc catgatacca taattggggc aggcacaaaa
    13321 atggataatc ttgttcatat agggcatgat acggtaattg gtaaaaattg tctttttgca
    13381 ggacaggtag gtatagctgg ggcaaccacc atagaagata atgtgattct ctggggacag
    13441 gttggtgtaa gtaaaaccct tacaatagga aaaggggctg ttgtatatgc acaaagtggt
    13501 gtcaaagatt caatagaagg gggtaaagtg tattttggtt caccggtaga agaagcaaga
    13561 gagaaaatga aagaatttgt ttggataaaa aggatacctc agttatggga aaaagtgatg
    13621 ggaagtaaag ataaaggctg atagcaatga cctctaatct tagtaatgtc ttcttcaatc
    13681 cggtaaataa tcatagggca gcagatcggc aagattcttc caactccagt aaccctggtt
    13741 gatccagtct ttctgctcat agtaataccc gttttctgta attgttatcc agtttttaat
    13801 atctatataa gatatctggt agggtacgct tttcggtagt ttcattttag ccaaatattg
    13861 tttttcagga ctttttttgg tgtaagtgat actaatcttt ctcgggtaat atatttctac
    13921 ttcagccggc ccggtggtta tagtgtgtac cacactatct tctccaatac tgtcggtcat
    13981 attctgtaca taattataat aagcggtatc gtagacattt ttgactttaa taaacttttt
    14041 atcattgttt tcatccagca ggtcaacgat ccatccctcc tccagcagcg tgctgtcata
    14101 atagctatgc ataaagtgaa gcctggagcc ctcataggta tttactcttg ccttgctcca
    14161 taaatttttt aaactatcag ttccttccat ttcagaaaaa agacaatatc cgcgatatag
    14221 attaatgtca gtattataga aataaacaaa cgagtcaagc tggtagcgaa gctgatatcc
    14281 taatgcatta ttgctgatga gaagagcatc agtcgccatt actttcagcc ggtttgtttt
    14341 tttatagaag aagaatttca aaacctctgg attggtaagg acacattttg atgcattggg
    14401 agtagaacca ataaaattct taataaaaaa atcaccatat ttctcccagc cgtcagctac
    14461 ctcaaaactg cttttcaata ccacttcgcc aagactttta tcctctttta ccagcagtat
    14521 ttccagttta ttgttatcac tgatgcggat cgtttgagtt tggtaacccg tataactgaa
    14581 gatcaggtcg taacctccgg attttagaga cagtgaaaat tcaccatttt tttgggtcac
    14641 ggtacccagg gtagtattct ggcaaaaaac ggatgcattt gataagggtt cacgggtggc
    14701 ggagtcaagt actgtacctg taacaacaaa ttgagcagtt gcagtaattg tgaaaaatga
    14761 taaaaggctg ataaaaacaa aacgaagatt ttgcatagca tcaagattgc ggattaaaga
    14821 taatgaccgg atagctaagg ttgcacggca ttaatagtgc caaggtattt tttacaggca
    14881 ttttctatgg tttcttcaag tggggtaaag gaaaatcccg gaagggcctt tagtatttta
    14941 tcattttcaa aaaaagtctg gctttgcgcc actcttgcac tctctttggt taaaagtggt
    15001 tttttaccgg ttaaaagtga ctttactttt tctaaacgcc aggcgatagc taataaaaag
    15061 ggagttgttt ttctgccggg tctttttttc ttaaaattat cagcaattgt atcctgtaat
    15121 tttttaaaag gccaggtgtc accattgatg ataaatcgct gttcagatat atcactttcc
    15181 atacaatgta tgactgcttt tgccacatcc tctacatcta caaaaccatt gatacccgga
    15241 gtgtaccagt tgaatccttc atacacctgt ttgaaaatgg cacaactgct actatgccag
    15301 tcaccaaagc ccagtatggt acttgggttc aggatcactg catccaggcc ttcacttaca
    15361 cctctccaca catgcagctc tgctttgaac ttactcttgg cataatgtgt attcacttta
    15421 ctctcttccc atttcttttc ttcatttaca tgaccaccac cggctgttct tcctaatgcc
    15481 gctacggaac ttatatgcac taaccggctc acatttttct ctagtgccat atttaccaca
    15541 tttgcagttc cttctacatt tacctggtac atctgttccc ggtcttttct caggaatgaa
    15601 actatggcag cagaatgaat tacagtgtca acactttcca tcgcatcctg taatgctact
    15661 acatcgagga tatctccttc cacccattca actttgtccg ttatttcttt ggatatccag
    15721 gtgggtagct tattgccccg ccggatggct cgtacagcat aatttttttc caccagttgt
    15781 ttgatgatat aagaaccgag aaaaccggta ccgcctgtaa tgagaatttt tgctggcaaa
    15841 ttcataaatt aataagtggc gaagatactt cttgttaacc tgtttgccga tagccggact
    15901 tattaactgg tcagttatat ttataatatc cttctctggt ttttttacct aatttacctt
    15961 ctctcacttt ttcatcctgg atataagaag gtttcaatct ttcgggatta ttcatttgtt
    16021 cataaactga gcaactaact gcatagttca catcattgcc gattaaatcc atcaatctga
    16081 acggccccat tttaaacccg gtagcttcca tcaggagatc tatttgggca aactcagaaa
    16141 ccccttcttc tgctaatcgc aacgattcga tataataagg ccttgccacc ctgttcacaa
    16201 taaaaccggg tgagtctttg catagtactg gggttttccc catctgtttg gacagttcga
    16261 taatggtttc agttgtttgc cggttagtaa agtttgtatt tacaatttcc accagtttca
    16321 tgattgtggc cgggttaaag aagtgcatac caattaccct ttccgggtgc tgtatttttt
    16381 cagctatatg ggtaatagaa agggaggaag tattactggc aaaaatacat tcactgtgat
    16441 tcaattcagc cagctgattg aataaagcaa tctttgcgga tggcttttca ataatggcct
    16501 caataaaaac atcagccagg caggtttgta tatcatctgt aaaatgaata cgtttaaaaa
    16561 ttctttcttt ttccgctgca tttatctttc ctttcgctgt aagggtaaga aggctatttt
    16621 caatgccggc tcttgctttt tcaagtatcg ttgtattcag ctcatataaa atggtagcaa
    16681 accccgcttg cgctgctgcc tgtgcaatac cgctccccat ggtacctgcc ccacaaacac
    16741 aaatactgtt aatgttcata cagaaagatt atagctgtaa agatattggt tgtggatttt
    16801 tgtagttcta tttgtggtaa gtttctcata ccttaaagca aattttattt atggatacca
    16861 accaacctgc ttcttcagct tcgtataccc ctgctccggc agcaccgggc ttatttggca
    16921 caaagattcc atcttctgtc agttttgctg tcggggtgtt attattcttt atgccctttc
    16981 tggatattaa gtgtaataat atgtcattgc aaaagatcag cgggatagag ctggccacgg
    17041 gttttcatat taaaagtccc gggggtaata attcactaat gggcaatttt gagaatagta
    17101 ccggcaataa agcagagagc aaaggtgata aaaaggaccc taatatgttt gcattggcag
    17161 cattggtatt gggtattgga ggttttgtgg tatcactact taatcacaga gcaggtggtt
    17221 ttggtggaat gctgacaggt gcattatctg cggctgcgat gatcgggctg atgatggata
    17281 tcaaaagcga tatcaaaaag gagttgttgg gagggaaaga tggagtgacc attgctgttg
    17341 agtttactcc gtggttctat atcgctatta ttgcattcct ggcagctgct tttttctgtt
    17401 acaaaagaat tcaatccaca aagaattaga atactcttca gtattttatg gatattgtat
    17461 gctgtggcaa cgtataaaaa cttgttttaa atactgctaa tgagagcctg tattgccatt
    17521 ttattttcct gctttttttt agcaggttat ggtcagacat ttgaacgctg ggcacaggtg
    17581 gtcaattggg atggttttag tcattggtct cgttatatga tcacacagcc tggttttcag
    17641 ggtcctaatt cattacctgt tccacgcatt ggtaatggaa gtgcagacag cagttttgct
    17701 gttggtgtta cggggaatct tcatttttca aatggagata atacacagaa tctttctgtg
    17761 tttgcgaatt gcagcgtggt aaaagaactt gtttcaattg atcttatgtg ggtgccctat
    17821 gaacactata ctatgagtac tgctataaaa gaaaaaagac atgtattttc cccatactac
    17881 aacgaccatt ccactcccgg cgacattcat cttaatacaa cctttcagtt gttaaaaaaa
    17941 tggcgggaat ctattcaact cgcattgagg attggttttc ggtttccttc cgggagcgat
    18001 tttggtgctg ccaggtatac tgatgggcct ggttattatt ttgatatttc gatgggaaag
    18061 cctatcaaaa attcttcagt aaaatggata gcaatgatgg ggttttatgt atggcagata
    18121 aaatcagatc accataacca gaatgatgct ttattagtcg gtagcggatt ggagtggaat
    18181 aaaaaatcgc tgagacttca gacttatatt gcaggataca tgggctacct gcaaaacata
    18241 ggagatgatc cttttgtttt ccgggctaat atggaaaaaa aattgcacag aactactttt
    18301 ctactgggtt tccagcaagg tttgaaggat ttcgcttaca gcagcgtaga agtgggtgca
    18361 aaatataatt tccgctggaa aaataatatg ctggctaaat agcagaagta aactgcctca
    18421 gaaaccgcac atcattttcg ctgaacaggc gcaggtcatt aacgccatac ttcagcatag
    18481 ccggacgttc gatacccata ccaaaagcga aaccggtgta tttgttgggg tctatcccgc
    18541 aatttgataa tacattcgga tgaaccatcc cgcaaccaag tatttccacc caaccggttt
    18601 tcttacaaat attacatcca ccaccaccgc aaataaaaca ggtcacatcc atttcagcac
    18661 ttggttctgt gaacggaaaa taagaagggc gaaaacggac tttcacttct ttgccataca
    18721 tttccttgac aaagaaataa agggtttgct tcaggtcagc gaaggaaaca ttctcatcaa
    18781 tatataaacc ctctacctgg tgaaagaaac aatgacttcg ggcactaaca gtctcattcc
    18841 tgtacacccg gcccggcata agcatccgga tgggcagttt gcctttttcc atttctctta
    18901 tctgcacact gctggtatgt gtacgcagca cccaatccgg gttttgctga atgtaaaaag
    18961 tatcctgcat atctctggca gggtgatgtt ccggtaaatt caatgcaccg aaattgtgcc
    19021 agtcatcttc aatttcaggc ccttctgcca ctgcaaaacc gaggcgctgg aaaattgaaa
    19081 caatcctgtt gcgcattaaa gtaacgggat ggcgggagcc aagcggtagt tcatcgccgg
    19141 gcaagcttaa atcgatgttc gatgtttgat gttcggtgtt tgacgttgct tctttcagtt
    19201 cagcaaacct ggcctcaacg cattgtttaa actcattcag cagttgaccc gcttcttttt
    19261 tcttttcagg agctacattt ttcatctcac tcataatagc tttcacaata cctttagtac
    19321 cgaggtattt aatgcggaat tcttccactt gtttatcatc agtggcagaa aaagaagtga
    19381 tgtctttttt gtagccctcg atttgcttca acagttgttc cataagggtg caaagataag
    19441 gaatggcccg tagcgattat aagactgaaa gtgaccgatg gacctcgttt tgccattact
    19501 ccatatttcc ctctttctat tttccattat cttgcatata tgtcagacca tctcaatata
    19561 tccgattttc tttctcctgt cagcctttct gctatctctc atgacgaagg ctataaggac
    19621 gggcagcttg gtaaagtaat tgctgtttat gatgatgaat ttcctgatct tgatgaagtt
    19681 caactggtga ttgtcggatg tggtgaacag cggggcagtg gactaataca tggccacagc
    19741 gaagcaccgg ataaggttcg ccggcacttt tataatttgt attactggca ccaggatatt
    19801 aagattgctg atgtgggaaa tataaaggcg gggtcactat ttacagattc ttatgctgca
    19861 ttaaaaacag tggttcagga gttgatgggt gatggcaaga cggttatcat tctcggtggt
    19921 tcacatgacc tgaccttgtc gcagtactat gcgtacgcag aaaccaaaag aaccattgaa
    19981 gcaagttgtg tggatgcagt gattgacctg aaccttgatt cccccttccg tcatgaaaat
    20041 tttttgatgg aaatgctgac aggtgagccc aattttctca ggcattataa tcatatcggt
    20101 ttccagagtt attatgtgca tccccgcatg ctggaaacat tggataaact tcggtttgat
    20161 tgctaccggg tgggtagtgt gaaagagagt attgatgaaa tggaaccagt gatccgtaac
    20221 agtaatatgt tcagttttga tataacagcg attgccaata cttatgcccc ggccaataca
    20281 atttctccta atgggcttaa tggggaagaa gcctgtgtac tgatgcgtta tgcagggatg
    20341 agccctaata taaacagtat tggcgtttat ggctatgatg cccagcatga taaacatgag
    20401 ctgactgcta aacagatcag tcatatgctc tggtatgtac tggatggccg cagccgtggc
    20461 aaaagagaag cggcattgga tgaaaaagat tccttcaacg aataccatat ggcttttgca
    20521 gaagtggata ccatattctt acaaagcaaa aagaccggaa ggtggtggat gcaattgccc
    20581 gataaaaaat tcattgcctg cagctataag gattacctga tggccagcag taatgaaata
    20641 cctgaaaggt ggttgagagc acaagagaga ggatgatgag cagatttgca aatttgataa
    20701 tgtgcaaatg gaaatatttc aaaattattc ataatggctt catttattcg caccggtatt
    20761 tgttcctatg gtatgagtgg aaaattattc catgctcctt ttattcaggc tcatccgggt
    20821 tatgagttat cggcgatagt agaaaggcac aaccatgatt caaaagaaag atatccggat
    20881 gcaaaattgt atcgttcagt ggaagagtta tgtgctgata ctgatttaca gcttatcgtg
    20941 gtgaatactc ccacccattt gcattttgaa caggcaaaaa tagtattgca ggctggcaaa
    21001 cacctggtga tagaaaagcc atttgcggtg acggtgaaag aggcagagga attatctgca
    21061 ttggctgtca aaaataaatt gttcatcagt gtatatcaaa atcgccggta tgatggagat
    21121 taccgtgcag taaaaaatgt tattgaacaa gatttattgg gagaattaag agaagcggag
    21181 ataagatatg atcgctaccg tccaaccttt ggtggtaaac agcataaaga aggggatatg
    21241 cccggtgccg gtattttatt tgacctgtca ccgcatttgg tggaccaggc catacagcta
    21301 tttggctttc ccaaagctgt ttttgccgat atatggaaga tgcgggaaga tgtaattgcg
    21361 ccggattact tcgaaatact tttttattat ggtaagctaa gagtacgatt aaaggcaacc
    21421 tgcattgcaa gagaaacaac atatgcttat accctgcatg gtatgaaggg aagttttttg
    21481 cagcaccggt ctgatatgca ggaacaacaa ctgcagtccg gagtcaagcc ttcattggaa
    21541 aactggtgtc ctgctcctgc aaaaccggat ggtttgttgc atacggaaat aaacggtgaa
    21601 gtaataagaa aagagacaac ttcctctccc ggcaactata tgggttatta tgatgatgta
    21661 tataaagctt tgacagggca gggtcctaac ccggttccgg ctgcggacgg tataaagaat
    21721 atgcggatta ttgaggcagc gttggagagt tttgcttctg gtaaaattgt ttcattataa
    21781 tttatgaatt atgagttatg aattcagtat tacagggaaa aagagattat ttaaatgtat
    21841 taagcaattc atcattctaa attcatcatt catcattctt ttatcttcct gttctataga
    21901 aaaacaaata tcaaaatctg cccgtgcaga tgttcttaat acaaaagccc tgcaagccgc
    21961 ccatgtcggc atcagtattt tcgacccgtc agccaataaa tacctctaca attaccaggc
    22021 cgataaatac tttgtaccgg ccagcaatac caagatacca acctgttatg cagcgatgaa
    22081 atatctgggg gatagtttgg tggctgcatt ggtattggaa aatgatactg caatattaat
    22141 tactcctact ggtgatccta cactgcttca ccctgaattc aaaattcagc cgctgattaa
    22201 tctgcttaaa aaagaaaaga gacaaattta tatcaatgat gataattggc aagatgaagc
    22261 cttgggttct ggctggtcat ggaatgatta tgaccaaagt tatatggccg aaagaagtcc
    22321 gctgccggta tacgggaata ttcttgagtg gacgcaggaa gtaaatgaga ccaataaacc
    22381 tccgttggtt tattcaagtc ctgaaactaa ttgggaagta tcattttctg ataatgaaaa
    22441 agccaggacc ttttcagtta cgagaagtat tgataaaaat tattatacta taacgctggg
    22501 agtagaaaaa aagagaacaa ttacaattcc atttgttact aacagggttc aatctgcatt
    22561 ggaactaata aaggatacaa tttttaagga aatcaataaa atagaactga ataaaatatt
    22621 aattcctggt catgggttta caaatagcct gaaaaagatc aactcccaac ccaccgattc
    22681 attgcttaaa cctatgatgc accgcagcga taattttttt gcggagcaat ctttgctgat
    22741 ggttagcaat gaaaggctgg gagtgatgaa tgatgctaag ctcattgata cgattctcaa
    22801 atccgatttc aaagacctgc cgcaaaaacc aagatgggta gatggttctg gtttaagccg
    22861 ctataattta tttacacctc aggattttat tgccatctta aataaaatga aaaatgaatt
    22921 tgggatggat cgtatcaagg ttatctttcc tactggtggt gaaggaacca tcagcagtta
    22981 ttataaaaaa gacaatggtt atatctatgc taagaccggc acacttagcg gtgttgttgc
    23041 tttcagtggt ttcttgtaca ctaagaaagg aaagctgctg atattttcaa cattggtcaa
    23101 taatcaccag gcatcagcta cggatgtaag aagggctatt gaaaaattct tgcagagagt
    23161 tcgtaataag tattgattgt caccctgagt ttatcgaagg gtgattcatt aaaccggctt
    23221 cggcaagctt agcctggtat ccatcacttc ccaaacattc cctccagttg ttcctgctta
    23281 aactctaaat aggtgggatt gccatccggc gttacattcg attgttcgca ggcaaatagg
    23341 tcatttacca gttgccgcat ttctctttct gtcaatcttg tgccggtctt gatggattgc
    23401 tgcctggcta aagaacgtat cagcttttct cttttagaaa atttgacttc attactgaag
    23461 tgtttgtatt gctccagcag gatatcaatg atatgttttt cattacctgc ttccacgtca
    23521 gccggtgtac cctgtatgac ataggtattc ttcccaaaag gttcaatgga ataaccgagt
    23581 tgttgtaaat cagtgattat ttcttccatt actgcagcat cagctggtgt taattccaat
    23641 gtggaaggga acatactccg ctgcgtggcg actggtttat ccttgctggc tgtttttaac
    23701 tggtcataca atactctttc atgcgccgac tgctgatgaa taagcagaaa accatttttt
    23761 gaaggaacag tgatatatgt attcaaaagt tgggtgagtt cattgaagtc tgaagtatgt
    23821 tgtttgaagt cagataggga ggatgctgaa ctttcgggaa cctggcttcc ggcctctgac
    23881 tgctgccctc cttcataaaa atctttccaa tgtttcaact cacttctttc acttttctct
    23941 ataaaatgtg cctggttctt ttgcgtaaaa cccttgaaaa tggaacctga tgcagcagct
    24001 gatttttttt cttcggtaaa aggttgttgt atagaagata actgttgtat agaggcatcc
    24061 aggtcaaaat ccagggtggg agttatgctg aattgtgcca atgcatgttt gacagccgcc
    24121 tgcacaaaag cataaatgat cttttcgtcc tcaaatttaa tttcttgttt tgtcgggtgc
    24181 acatttacat ccacgaatgt cgggtcgagg tcgataaata atacatacat agggaaattg
    24241 tccgaagaaa tcatttcctg gtaagcattc atcaccgcat ggttcaggta agggctttta
    24301 atgaaacggt tattgacaaa gaaatactgg tcacctctgg tcttttttgc cgtttccggt
    24361 tttccaacaa agccatagat attcatgtaa tcggtatctt cttttactgt taccagtttg
    24421 gcattgtaat tattgccgag taattgaacg atgcgttgct ttaaactgcc ggcctccaaa
    24481 tgaaataact gctggttatt ggcggttaat gtgaatagca tttcaggaaa cgacatagct
    24541 accctggtga actcgtcaac aatatggcgc atctctgctg cattgctttt caaaaaattc
    24601 cgccgggccg gcacattgaa gaataaattc ttcatagcaa tgctggtacc ctgtggtgcg
    24661 gcaatgggtt cctgtttttt cactacacta ttctccactt cgatataaac tcccgtttca
    24721 tcctctgctc ttttgctttt taactctacc tgggccacag cagcaataga agctaatgcc
    24781 tcaccgcgga atcccatggt ccttatatga aataaatctt caatgttttt tatcttggaa
    24841 gttgcgtgac gttcaaaaca catcctggca tcggtttcac tcatgccgct accattatca
    24901 atcacctgta tcagtgattt acctgcatca gcaatgataa gtttgatttc cgtagccccg
    24961 gcatccaccg cattttccag caactccttt acggcactcg ccggccgctg aattacttct
    25021 ccggctgcta tctggttggc aatattatca ggtaataagg taatcttatc aggcacaaaa
    25081 tccgctttag ccgccaaaaa taaccatttc agttttaagc aatcattgac tcttgctttg
    25141 catcaaaaag agtaatttta agattcgtta tttaacacca taatttcttt aatatgacga
    25201 ttctaagagc cgcattactg tgtctttttt tcattccatt tgttgcaaca gcccagtaca
    25261 aaagcaaatt gaacagggag cagtgggttg atagtgtttt caatagcctg agtgatgatc
    25321 agcgaattgc ccaattaatg gtaatcaggg cacactcaaa tctaggtccg gatcatgtgg
    25381 ccaaagtagt aaatgatatc cggcaatata atgtcggtgc actttgcttt ttccagggtg
    25441 gcccaataag acaagctaac ctgaccaact attatcaaag tattgcaaaa acaccattga
    25501 tggtgacgat tgatggtgaa tggggattgg gaatgcggtt ggacagtgtc accaggtatc
    25561 cataccagct gacaatgggt gctattaatg atgagaacct tgtttacagg atgggagtgg
    25621 caatagggga acaatgtaag cggatcggtg tacatgtaaa ttatgctccg gtaattgata
    25681 ttaataataa tcccaataat cccgttatag gttaccgttc atttggagaa gacaaggaca
    25741 aagtctcaaa atatggggta gccataatga aagggatgca ggatgcaggt atcatggctt
    25801 gtgccaaaca ttttcccggc catggagatg tagatgtgga ttctcattac gatctgccgg
    25861 ttatcaataa aagcatgaaa cagctggatt cgatggaact ggtgccattc agggctatct
    25921 ttaatgcagg ggtgggcagt gttatgattg cacacttata tattccatcc attgataata
    25981 ctgcgaacaa ggccacatcc atttcaaaaa ataatgtaac cgacctgttg agaacaaaga
    26041 tgggatacga aggcctcact tttacagatg cactggaaat gaaaggggta gctaaatatt
    26101 ttcccggtgg caccattgcg gtagaagcca tcattgccgg aaacgatatg ctttgtttgc
    26161 ccgctagtgt accagagtca attgaaacga ttaaacaagc tattactgat aacagattaa
    26221 gctggtatga tatttatgct aaagtgaaaa aagtactggc gtccaaatac caactgggat
    26281 taaacaaatg gtcaccaatt gataccaata acctgctggc agatataaat gctaaaacag
    26341 atgctatccg ctatgaagta gcacaacaca gtattaccat tctttcacaa gccgggacaa
    26401 aagcctccag gaccgattat gcacaggtgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn ntataaggat ttaaatttca ttaagacgat tactctcaca tttggtaatg
    26581 tattagccac aaaaaatttc tgtgatgcat ggactttggt agcctgctac caggatgatg
    26641 ccattacaca ccaggcagct gcagacctgt tgcagggtaa aattgcagca cagggaaagt
    26701 tacctgtttc tgtatgcagt tttaaatttg gagagggcat tatgcaggga agtacacaac
    26761 cttcaaaagc cctgattaag gaaaggttgc aggtagttga ttcaattgcc cttgatggaa
    26821 tagccaaaaa agcatttccc ggttgtgtgg tactggcagc gcataaaggg gagatcatct
    26881 atcataaagc atttggaaat tatgaatatg atccggcttc accggttatg agtgcggaga
    26941 gcatttttga tctggcttca gtcaccaagg tttcagctac agctgtttca gtgatgaagt
    27001 tgtatgaaga aggaaaaatt aaactggatg gtaaactgaa agattacctg ccctgggtaa
    27061 agggaacgaa taaagaagac ctgaggattg atgacatctt attgcaccag gcagggttgg
    27121 taccttttat tgcattttac aaagaaacga ttgatgctgc caccggcacg ccaaatccgg
    27181 ctatttatgc tgccaaacca caaaagggat acggggtaag agtagcggaa aatatttatc
    27241 tccgggatga ctggaaagat acgatgatga tgcggatctt aaaaagtcct gtaggaccaa
    27301 aaaataaata tgtgtacagc gacaatgatt ttattttcct gggaaagatc attgaagaga
    27361 taacagggat gacgctggac cagtatacac agaaaacatt ttataataag attggaatgt
    27421 attctacagg ctttaaaccc cgtaatcgtt tccagcttga atgtttggtg ccaacggaaa
    27481 cagaaaagca tttccgccag caaactacaa gaggtgatgt acatgatgaa ggggcttcta
    27541 tgtttggggg agtggcgggg catgcagggt tattctccaa tgcatatgat ctgtccctgc
    27601 tctaccagat gttattgaat gggggggaac tgaatggcga acgatacctg aagccggaaa
    27661 cgataaaact tttcacctca tataatagta aaataagccg tcggggactg ggttttgata
    27721 aacctgagaa agataatgcc atccgcaaag atccctatcc ttcattgctt gcgtcgccgg
    27781 aaacttttgg tcacaccggc tttacgggta cctgtgtatg ggtagacccc aaacaagatt
    27841 tggtttatat atttctctca aacagggtaa cccctacaag ggataataat aaattatctc
    27901 aattgctgat acggggaaaa atacaggatg ctatttacag ggcgttgggt gttgaatagg
    27961 ataatttttt taatttattt cttctcgggg ggtaaataat ctataatatc ataaatagat
    28021 aacttgtaaa actgtgccag taaatgaagc cgttgaatat ctaatttagt tttgcctgat
    28081 tccagaagag aataggcatt ctggctcatg tggagttgtt ctgcaatctc ttgttgtgtt
    28141 agccctttct ttttccgaag ttgtcgtaat ctttcaggta cattcatttt caatctgata
    28201 caatttgttt tatgaatatc caggaattca gacagactgg caataccact ttgtggtata
    28261 ttttatcatt tatttggata tgacctattt atcagtaaaa cggtttgaaa aaaaattaac
    28321 ttgtaatgaa attgcttttg atatcaattt attaaacctg aaatcaaaag tttatgaaaa
    28381 aaacatgctt attactagga attgtattag ttctttttaa tctatcatgc gataaagaag
    28441 aaaaagggca gaaggttgat gtgaaaaggt taaccgttgg aaatatactt agattcggaa
    28501 gtgtaaacgg taattcttct gagttatttg ctcccatcga aattggggaa ctaccgggag
    28561 gggggtcatg caattgtaat ggacatggag gatcaagtgg tggaattctg tcatggactt
    28621 tagccacttg cagatcgaac tgtacccgag gtattgggtt taggtgtggg agggaaggga
    28681 ttcttttttg ccaggatggt acaacaataa tatgcacatg gggtagtaac tgcccaagtc
    28741 aggcaaaagg tactgtgcca gaaagggata tggaagcttc ttatatgttt tatgataatg
    28801 gcacaatgaa attaactttt aacaagcaaa ttcccgatct agaaaaaaat gccgcaaatg
    28861 gagacatatt tgaagttgag tcgactgatt atgtgccttt ttcatcagca gtttcggtag
    28921 acggggttta ctatactggt tttgattttt tgcaaggtaa ttatcatatt gactataccg
    28981 atggtcaata tggtagcgtt gtcatcggta ttcaactgaa acaataaata aaagggatat
    29041 ttctaacagg aagtatccct tttaatacaa catatgaaat tcatattatc tgctataata
    29101 gttttatact gctctggctg tagtgtcgtt gcacgtaaag gatatggcgc taaaaaagta
    29161 aaaatcgaga atgaacaaac aattcaaaat tggcttagtg aaaaaggctt tagtaccagg
    29221 aatgtagtaa cagttacccc ggagagttat tacgataatt attttggact cccccaggct
    29281 ccccttttat ttgataaagc ttcgggtaat tttttggcca ttgggtttac taacggagaa
    29341 tattgtccca aagatgtaga caaatcgttt tcatccatac cccactatag tttaatgaaa
    29401 gtaaaaccag acagtttttt gatttcagaa accacttcaa taccgcctgg tttttcaatt
    29461 aaagacaagg ataagtttga gaaaaagata gatactttgc ccataaaact tacccaaata
    29521 acttcatact tcagaaccct gaaaggcgaa ccctctccgg agtttttaca aaaaaaatca
    29581 gactatattt tgatcattcc ttttgccatt ttcctcggta aaaaattaca ggtaaaggat
    29641 cttaaaaaat attattactc agcacttacc aaccggtcag caatgataca aattgtattc
    29701 ctcaatcttg acaaacaaga gtggtggggc gaggaatgga ataagaaaat aaatattacg
    29761 tattagtgga aggtttatcc tttccctggt taggattatt cggccgctgc tggttaccac
    29821 tttgctggcc tgactgacgg tcaggttgat tacgttgatt cctgttagga ttattgggtc
    29881 gctgctggtt accgccttgc tgtctttgtt gcccgccttg ttgtcttctt tgttgatttt
    29941 gctgaccgcc ttgttgccca ccttgctggt ttccctgctg tctttgctgt ccaccctgtt
    30001 gtggtcgttg ccccctttgt tgttgctggt tctgacgccg ttttttatca gcagctttat
    30061 caagactttt taaactgatc tggcctacca gttctacaaa ctccggttct gcttctttcg
    30121 gtttgcccga tgttacttct accggctcca ggtcatctgg ttttatacct tgctggttca
    30181 gtgattttat tttctgcacc ctttcaatgg tcaatggata ctgtttatta ctatcgggca
    30241 atgtgtacca catcaggttt ttgaagatat ctttcttgat caggaaggca ttccctttgg
    30301 ctacctgtat tacatcacag ttatcaggga aatgctgtaa ggcatctaaa taagtatcca
    30361 gctcatagtt caaacaacat tttaaacggc cacattgtcc gcttagtttt gtctggttga
    30421 tggataaatt ctggtaacgg gcagctgttg tattcaccga tgaaaaatcg gtcaaccagg
    30481 tactgcagca aagttcacgg ccgcaactgc caataccacc cactttaccg gcttcctgcc
    30541 tggcgccgat ctggcgcatt tccactttta ctttaaattc cgaagcgtaa actttgatca
    30601 gttcccggaa gtctacccgg ccatcggcta tataaaagaa ggttgctttt cgtccatcag
    30661 cctgcatctc cacctggctt attttcatgt ccagcttcaa ttgcttggca atggcccggc
    30721 tgcgtattac cgcttcaggt tcccttgctt tattttgttt ccagatatca atatcacggt
    30781 cggtagcaac ccgcaacact tttttcattt ccggattatc ttctttcaca ttccttttct
    30841 tcatttgcag gcggacaatc tcgccggtca atgatacttc acctacatcg aaaccactca
    30901 ccccttccac agcaatcagg tccccttttt catacggttg gagagtcggg ttgcgataaa
    30961 aatctttacg ggtaccctgg ctaaagctta cttctatcac tttacaggca ctggccacat
    31021 cacttatcgg caggttgcgc aaccagtcat gcgtattcat acgattacat ccgccggtgc
    31081 tgcacccgcc attactctta catccgcctg gtttaccgtt tgaagcggta ctgcaacttg
    31141 tacaactcat ttattcaatg aataattaag aattaaataa ataaaggaaa tgatgaaccc
    31201 ggcgaaagaa ttactataaa gcttcagttg cgtcgcactc ttgtacgttc tgttcagtac
    31261 tccacaaagc tattttgcat tgaacagcag tactatatgt aagagttgtt gtagacattc
    31321 agtaattcgg tatatcaggg ttcttcctcc tttccacatc ctgttcatca ctcaccataa
    31381 actcaattca ccaaaaatac agttttgtcc tgaatgatat ggtatagttt aatggtcagg
    31441 gcatggaaga gcattttgcc atgggcattc cgttctatat aataactggc ccggtcaagc
    31501 tcctcaataa tggcttgttg ttgctcaata ccggctattt tgttcaatct ttcggcaaaa
    31561 tccctctctt tttcgccaat attgagacgt tcgcccatta tccggtaatg aatggcctgt
    31621 tccaataaat ggttaaaata ccggagaaac tgcttttgct tttctcttcc caggcgactt
    31681 gcctcctcta cccatttggt ttgtgcaaca gggccggtct ttaaaatagc attcaaccat
    31741 tcccggagca aactctgcca gtcttcctcg gcatgttgca ccagttgcaa tgcttcccgg
    31801 taattgccgt cgctaacacc cgctacctgc ctggcgatag ccggttcggt tttattacga
    31861 tttatcaggg cttcctcaat atcccgggta gccaatgccg gaatttttac taactggcaa
    31921 cggctgacaa tagtttgtaa aacctgggct tcattttccg taaccagtat aaatagtgta
    31981 ttgggtggcg gttcttcaat cagttttaaa agtttgttgc cctctgtgcc cagcatttcc
    32041 ggcatccaca taatgaggat cttatattca ctctcaaaac ttttcaaact cagcttgcgg
    32101 atgatgtcat tgcattcttc ggcggtgatc tttccctggc tgttttcttt ttctttgatc
    32161 agctcaatcc agtcgaacga attgccgtaa gggttcaatt ttagaaattc ccgccattcg
    32221 gcaatgaaat cagtggcaat aggtttctct cctggttttt tcgtcacggt gggataagaa
    32281 aaatgaatat ccgggtgaac aaactgttga gctttaaggc aggctgaaca tacgccgcaa
    32341 ctgtctgttt gtagtttgag gttcgaagtt tgaggttggg ggtctccgaa taacgaggga
    32401 cctgcctgga tggacgtatt ctttccgttt actttctcac aaaccacata ttgtgaaaat
    32461 gccaatgcta atgataagga cccgcttcct tctttaccca aaaacaataa tgcatggctc
    32521 agccggttat gttgcaccag ttctgccagt tgttgcttta cttcctgttg acctataatg
    32581 tcggcaaatt gcatgggaag gcgaaagtaa actttctgcc actaaaaaaa agagactgtc
    32641 tcaaaaggta gtttgattgt catattgagt ttatcgaatt attggcaatc aaaccgatag
    32701 ccttcggcaa gctcaggctg acatatttat tgagacagcc aattttgtca attgaaattt
    32761 tatcagttca gatacttgag tatatccggg ctcataggca ctgttctcgg actgaaagta
    32821 gcaatcagtt cacctttttc attcaccagg aactttccaa aattccaggt tacagggtcg
    32881 gcaaaacctt tggcagctgc ctgggcttgt aaatatttat atagttcatg aatatcatta
    32941 cccttaacag atatcttttc tgccattggg aatgtaacgc cataattctt tttacagaat
    33001 tcttttattt ctccgttgct gccgggttcc tgaccgccaa agttgttggc aggaaaccca
    33061 atgattacca gtttgtcttt atactttttg tacaattgtt ctaatccttc atattgggga
    33121 gtcaaaccac attctgaggc agtatttacg atcatgatct ttttcccttt gtattgggca
    33181 aagtcaatat ccttaccatc cagccctgct attttaaaat catatatcga accgtcggtt
    33241 aaagttgctg ctaagaccat agagagaatg agtgttttca ttttatttaa tttcaaagtg
    33301 aataacaaaa ctaagtgtaa aaagtttgta gagggtagtg agtggcgcta ctgtttttca
    33361 aagcatccta aatccggcaa acctactggt cgtgttttcc catctaaatc agtgattact
    33421 gtagtgttga cacctttatt gatagccggt gatgaagtcg ccagcctgaa atcgtaataa
    33481 tttcttgatg tattaatgct gtcaaattga ggcgattggt tattgatgat ttgattgctg
    33541 gtgatattag aagggatagt ttgtactttc cagagattat gatcaaactc tacattgaaa
    33601 atg
//
LOCUS       DAORTI010000023        33238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_878_length_33238_cov_8.267366, whole genome shotgun sequence.
ACCESSION   DAORTI010000023 DAORTI010000000
VERSION     DAORTI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 33238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33238
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_33238_cov_8.267366"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..800
                     /locus_tag="PLO70_05075"
     CDS             3..800
                     /locus_tag="PLO70_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQZ73862.1"
                     /translation="MTDYNKDGYLVVKNFLDANEINKLQQIAFDDGAMRKHAFDLNDQ
                     AGKKTKLTLWYKPGNDAYGLLTRSDRMVNGADSLMEGESAVCHFHSKLMQKEPKVGGA
                     WEWHQDYGYWYKNEFLLPGQMISIMIAITDAGKENGCLQVIRGSHKMGRVEHGFAGEQ
                     VGAAQRYVDLALKTMELVYVELKAGDALFFHPNILHRSEANLSDNARWSLISVYNRSS
                     NVPYNEPSQSSTVPVEPVPDEALLNWKEELIDDSANFLEKEKDQALK"
     gene            808..1224
                     /locus_tag="PLO70_05080"
     CDS             808..1224
                     /locus_tag="PLO70_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQZ73863.1"
                     /translation="MFFSSSPVWQTSGLTLIRLTLAGFLIYHGWEIFNDTKMNEYLAW
                     DNFKQSNGKLLVYAGKAAELLAGILFVLGLFTRIASLLTIGTMCYITFMLGNGIIWNN
                     DQHPFLFVLLALVFFFTGAGRFSLDQYLFSKKSLYL"
     gene            1224..2951
                     /locus_tag="PLO70_05085"
     CDS             1224..2951
                     /locus_tag="PLO70_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQZ73864.1"
                     /translation="MSDSLQVHINTKGKEANSYDAIVIGSGISGGWAAKELCEKGLKT
                     LVLERGRPVDHIKDYPTAHLSKWELPHRGWITNETKQKNPLISKAAGYSEDTQHFFVK
                     DADHPYVQEKPFDWIRGYQVGGKSLTWGRACARWSEFDFTAPKRYGYGESFPIGYKDI
                     KDWYSHVEKFIGVCGSYDGIPSMPDGDFLTAYELNVVEKHMQKVLKEKFNRHYVSGRW
                     AHITDPKQIHLDQGRAQCMNRNLCMRGCPLGGYFSSNSSTLPWAAKTGNLTIRPHTVV
                     HSIIYDEQKQKAVGVRVIDANTKEATEFFATIIFVNASALNTNLILLNSVSDRFPNGL
                     GNDSGLLGKYICFHNYRGGMWGYMQGFEEWYYKVSKPAECIIANFRNVEKQETDFYGG
                     YVIYSGASREKLNDHSAPEPVGASFKETLSQPGRWSVYMYMQGETIAKQSNHVRLSKE
                     EKDQWGIPLLITSVDYDDNDDKMVKDFLEQGKAMMEAAGIKEVNTWDTKQPPGLDIHE
                     MGGVRMGSNAKTSLLNDWNQLHHCKNVFITDGACMTSTGSQSPSILYMAFTARAVDHA
                     VSELKRRNL"
     gene            2953..4716
                     /locus_tag="PLO70_05090"
     CDS             2953..4716
                     /locus_tag="PLO70_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQZ73865.1"
                     /translation="MKRLVTGIVLVMSLCTTHAQQKTYCNPINIDYGYTPIPNFTEWG
                     RHRATADPVIVTYKGDYYLFSTNQWGYWYSSDMLNWKFNARKFLRPWNEGYDELCAPA
                     VGIIGDTMIVFGSTYTSKFTIWMSTNPKANEWKPLVDSFDIGGWDPSFFTDDDGKLYM
                     YNGSSNKFPMYGIELNRKTMKPVGTRKEMYLLEPWRFGWQRFGEHMDNTFLDPFIEGS
                     HMTKHNGKYYLQYGAPGTEFSGYADGLLVGDNPLGPFEAQSDPVSIKLGGFVRGAGHG
                     ATFRDNYMNYWHISTTVLSVKNTFERRLGIWPAGFDAEGKMFCNTTFGDYPHYIPAPG
                     FSGIAYGVNGKSAYFTGWMLLNYNKPVQVSSTLGGYLPNNAVDELAKTYWSAESGNKG
                     EWIQTDLGNISTVNAVQINYADQDVESNRLGKWDNQFHQYKLYYSVDGKKWVVLFDKS
                     NNKTDVPHDYVELEKPIQARFIKLENIHMPAGKFAISGFRVFGNGNGAKPDPVQGFIV
                     LRTEKDKRSAFIKWKPVDNAFAYNIYYGTHPDKLYTSIMVHSNNEYWMKAMDLQKTYF
                     YCIESINENGTSERSKVIKVD"
     gene            4676..5383
                     /locus_tag="PLO70_05095"
     CDS             4676..5383
                     /locus_tag="PLO70_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQZ73866.1"
                     /translation="MEPVKEVRSLKLIDMMKKVINLVIVFFLLTLSVQSQEKISFWNE
                     VQAFRQKDSVQFPAAGQLLFIGSSSFTMWKDVQDYFPGYKILNRAFGGSTLVDLIRFR
                     YDVIYPYQPKQIIIYCGENDFAASDTVTVEMVMQRFKTLYRLIRAKYTSVPLVYVSMK
                     PSPSRVHLMSRYAEANRQIEIFLNKDKKAIFADVYSKMLKENGSPMDDIFLTDSLHMN
                     AKGYAIWEKVLGKFLIK"
     gene            5393..6445
                     /locus_tag="PLO70_05100"
     CDS             5393..6445
                     /locus_tag="PLO70_05100"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQZ73867.1"
                     /translation="MVRLRKFVFPLLLLSVCGMSCKQETAVTKIAFGSCGHQSDPQPV
                     LAIAAEQNPDAFIFLGDNIYGDTDNMDTLRNKYERLGAKPEYRQLAAATKIFATWDDH
                     DFGRNDAGKYYPFKKESKEIFMEFFKEPANSDRRKHDGIYKAEYLQEGDKIIQLILLD
                     VRSFRSDLLLFDKENKELQARYFYPLDYQPHTVPDSTLLGDDQWKWLEEELKKPADLR
                     LICSGSQFGIEFNGYEAWANFPLEQKRMLELIKSTKANGVLFLSGDVHYAEISRLETD
                     GLYPIYDITSSGITSTWSFATPNKNRIEGPVMDNHFGLLTIEWEKDPVIRMEIIDKNK
                     NNRIEYTINKSQIQFK"
     gene            6474..8765
                     /gene="bglX"
                     /locus_tag="PLO70_05105"
     CDS             6474..8765
                     /gene="bglX"
                     /locus_tag="PLO70_05105"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HQZ73868.1"
                     /translation="MKRAVLILIVISALLAGKEVTGQTTNNAKMKSFIDALMKKMTLE
                     EKIGQLNLPGSGDIVTGQAQSSDIAKKIKEGKVGGLFNIKSVAKIRDVQKVAVEQSRL
                     KIPMIFGMDVIHGYETVFPIPLGLSCTWDMNLIERSARIAAIEASADGINWTFSPMVD
                     IARDARWGRIAEGSGEDAYLGSQIAKAMVKGYQGNDLSKNNTIMACVKHYALYGAAEA
                     GRDYNTTDMSHQRMYNEYFPPYKAAVEAGAGSVMASFNEVDGIPATANKWLMTDVLRK
                     QWGFKGFVVTDYTGINEMIDHGIGDLQTASARALMAGIDMDMVGEGILLTTQKSLKEG
                     KVTLAQINAACRRILEAKYMLGLFDDPYKYCDENRAKTEIFTAAHRKEARSTAAQSFV
                     LLKNQNNVLPLKKAGTIALIGPLADNKENMPGTWSVAANFDKATSVLTGLKEVVGDKV
                     KILHARGSNLDEDSLFEERAGMFGKSLRRDSRPANVILQEALDIANQSDVIVAALGES
                     SEMSGESSSRSDIQIPKLQQDLLKALLKTGKPVVLVLFTGRPLALKWENENVPAILNV
                     WFGGSEAGYAIADVLFGDVNPSGKLSTTFPQNVGQVPIFYNHKNTGRPLEEGKWFSKF
                     RSNYLDVSNDPVYPFGYGLSYTSFSYSDIKLSSASLKGNQSLTASVTVTNSGKYDGKE
                     VVQLYIRDMVGSVTRPVKELKGFQKIDLKAGETKTVTFNITPNDLKFYNYDLKFDWEP
                     GEFQIMIGGNSRDVKVGKVNWIK"
     gene            8809..10620
                     /locus_tag="PLO70_05110"
     CDS             8809..10620
                     /locus_tag="PLO70_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQZ73869.1"
                     /translation="MEKTRILLGLLLLPFAVSSQGLIINTFNRNTTSLNGEWNYIVDP
                     YETGYYNFHSEIYDQKNAGSPSAFYNNYHAKNKAELVEYDFDKSPVMVIPGDWNTQKE
                     NLFYYEGTVWFKRSFDYALKEKNRLFVYFGAINYKADVYLNGIKLGTHEGGFTPFNFE
                     ITAVVKPAGNYLVVKVDNKRFREAVPTMNTDWWNYGGITRDVILIEEPPVFVQDYAIQ
                     LKKGTQNLIAGYVKLNGLTGGENVTISIPELKIEKNITVSNNGYTPFEIRTDKLQYWS
                     PSNPKLYEVSIKTSRQTLKDNIGFRTVETKGADILINGMPVFLKGICMHEEYKGRRAN
                     SEADARAMLTWAKELGCNFIRLAHYPHNEYMLKMADKMGLMVWEEIPVYWTIDFGNES
                     TYNNAKNQLTEVITRDKNRACVIIWSMANETPLSDKRNYFLKNLVNHARSLDSTRLIS
                     AALLTRGEKGVGIIDDKIGEYLDIVAFNQYRGWYGGDLATAPDAKWVTPYNKPVVVSE
                     FGGDAKQGLHGDREERWTEEYQEYLYQQNLLMIEKMPNIRGISPWILTDFRSPRRMLP
                     GIQDGFNRKGLIADNGMKKKAFFILREFYKKISLKYK"
     gene            10638..12614
                     /locus_tag="PLO70_05115"
     CDS             10638..12614
                     /locus_tag="PLO70_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HQZ73870.1"
                     /translation="MKQLLTFFIALVLCFNGFAQRPFLLYSPDSALRLEIITGTQLAY
                     KLFAGTQLLQSSSSVDLKLDNGLQLSDKLVVSMYKYRKVNELVNVAVPYRRPVISNHY
                     QHLELIFKQPFSVEFRLYNNGMAYRIGTRFKDSIIIKYETAIFDVDSQSVVWFPHIDK
                     RPEADRFHTSFEGIYKKELLSGIPDSMLTYAPVTLSLPNGYHLSITDANLYDYPGMFL
                     QTANSGLQGVFAPYPLREVMTEGEFPQFMVTERANYIAKIKGTRMLPWRAVLVAKNDI
                     DLPENDMIYLLGDPTTLTDFSWIRPGKGTDEWITGINLFNVPFEAGINTATYKYYIDF
                     ARRFGFDQVMLDAGWSDYRDLFKINPNLDMDELVAYAKKNDVKLMLWTLCSTLDKQLD
                     AALAQFNKWGVSSIMTDFMDRDDQPMVNFYHRIAKACAEHKISIMFHGAFPPKGFNRT
                     WPNAITQEGVLGAEWNIWSDLATPEHNVAIAYTRMLAGPLDYEPGLLLNAQKDQFRPI
                     GKNPMSIGTRCHQLAMFVVYDSPLQIFSGNISQGLKEPVFMEFLGSIPTTWDETRILQ
                     GKIGEYIITARRKGNDWFIAGLNNSVAREIDLSFDFITEGDYKMIVCKDGLNAHNYGS
                     DYVLNEMLVNKSTEYNLKMAPGGGFLVKLVSKTK"
     gene            12614..13441
                     /locus_tag="PLO70_05120"
     CDS             12614..13441
                     /locus_tag="PLO70_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQZ73871.1"
                     /translation="MDSRKIIKAIIGAGIGLLFSCSPTRKNSMSPTFAANPVVAHRGA
                     FKKNSLPENSIASLKEAIRLGCTGSEFDVRMTADDSLIINHDPHFNKLEIEKTTYLEL
                     TQFKLSNGEKLPTLTDYILAGLDKNLQTRLVLEIKPSGVSKERGKQIAEKVVKLVKEL
                     NAGAPIVYISFDYDILKRVIELDPKASTQYLNGDKSPAQLKADRIDGADYHFTVFKKN
                     PDWIEQAKKNKIILNAWTVNDAATMDWLLANGFDFITTNEPELLFERAKLSPIISGN"
     gene            complement(13468..14505)
                     /locus_tag="PLO70_05125"
     CDS             complement(13468..14505)
                     /locus_tag="PLO70_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ73872.1"
                     /translation="MKKNNVTIQSMAKTLGVSVTTISRVLNGLGEQFRISKKTIELVT
                     TTADKLNYRPNNIAKGLRLKKSSTIGLILPDITNTWFAQLALGIEKEARKHHYNIFLC
                     NSNDDIKVEKKSIALLQSWMVDGIIIAPIGLESEHLQKASKSGTPVVLIDRFFEGVNL
                     PYISSNDFEGSLEATQYLINNGHKKIACFQGIVGTSPNNQRVNGYKQALKNNKIHFDP
                     SLVMGEDFGFNNGYTCAKKLIKHLAKSKITAIISMGNQITLGILKALKEEGVQIPNDL
                     SIISFDEQEYSDLLYTPLTTVSHMNENIGDVALKMLFNQFGKSTRIKPKNVVLNSKLI
                     IRDSVKKIGPG"
     gene            14724..>16519
                     /locus_tag="PLO70_05130"
     CDS             14724..>16519
                     /locus_tag="PLO70_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQZ73873.1"
                     /translation="MKKIVQGGSLFFLLLISSLAWSQNKTVTGRIIGENGIGVSKASV
                     LVQGTRIGVTTNERGEFTISVPSSATILVISSVGYGTVEEPINGRTAIEITLKPGVQE
                     MQEVVVTALGLKREEKALGYAVQKVSGESLQRVAGLDVATSLTGKVAGVLVRNSPDFA
                     AVPLVTVRGENALLVIDGIAYQNKTLTDISAEDIESVSVLKGATASALYGFRGANGAI
                     LITTKNGSSNKAGVTIDYTTNTMFTAGFLAIPEKQAVYGRGGSGIYALNSDQSWGPKM
                     DGSMQNQWDPVSKTFTVRPYLPVGKDNFKNFLEQGYVTNNNLNIGYQKDKISLRNSFN
                     WTENKGRYPNSKLQKYTYSFGADLNYDKFKMSTNLSYAKKASKNIGSNGYTSYDPMYS
                     ILIWSPVDWDLSLYKNNYWIKPGEIQNNHFGLVPGGTIDNYAGVNQNNPYFDRYEKTN
                     EISRDIFNADLTMSYQLTTWLKATVRSGVDFYKETGKLRSSWGSYLSSGNTGVPGADY
                     SGWNGGRKGQYNIGVSNGFSNNTDFMLTGDRKFNKFTVEYLAGGTIFYRRDENMNAST
                     QGGISVPGFFSIKASVNPAQVSQSTYAQQVNSV"
     assembly_gap    16520..16619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16620..17756
                     /locus_tag="PLO70_05135"
     CDS             <16620..17756
                     /locus_tag="PLO70_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ73874.1"
                     /translation="NSSYSISAATWGALNGAGVPGNLYSPTIAPTSSETYEQGLQAMF
                     LKNRIMLDVSHYTKRVFDRITTVGLTGASGYGGLVTNFGEEITRRGWEVILNGSPIKN
                     KDWQLDLGINWSTFASYYTKLDPVYSVNKPWVKVGERADHFISRDFQRVPSGKGDQSG
                     ALIHNSGGRPIVHGYDVLFGYYDPKFIWGTNANLRYKNLRFFLSFDGVNGGLANTRTE
                     SYMWQSGVHPNSLSPERALDVATPGSNNYMGQGVIVVSGAATYDANGNILTDTRVFAP
                     NNIKTTYKQYMIDLHNSSAWGGNGSKADTYSRTFFKLREISLSYNVPAKLLHKVAKAA
                     SISLIGQNVLLKAKQFKYSDPDGGNEDFADPSTRYLGFKINFTF"
     gene            17769..19310
                     /locus_tag="PLO70_05140"
     CDS             17769..19310
                     /locus_tag="PLO70_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQZ73875.1"
                     /translation="MKKIHYILLATISFTIASSCKKFDQLNTNPDVPTSVSPDLLATT
                     VLKSTYRFWNPNPTDWGTAQLWSKHCTNLQNNPNPYQYFSSYYPYGGFGGAQNITILN
                     RMVDFAEGNPAKPSYEGLRLFLKAWYGFSATLDMGDVPYSEAGKAEEGITQPIYDKQA
                     DVFVGVLNDLKAAEEKFAVGVNFTGDIMLGGNAAKWRRLCNAMQLRVLQTISKKITPA
                     QKARFAEIVAAGNLMTGNTDNFQLAYLDNTNATYPFYNGEGMRVNLAMSKLMVDALKN
                     VNDRRLFYFGEPAAYKISGGLLENDFAAYEGAPSELSPNLLDLNRAAGKYSLVNKRYA
                     SSASRIGDPYIYFTYAEQCFIIAEAIEEGWVAGNAQTYYENGVKAMLDYYRTLPTVIQ
                     TNLHGMAITQTYIDNYFTGEAAYKTSGTKTERLQQVWMQRYFIDFFQGGVNISFRNFQ
                     RTGYPVFPLDPATSLNSGAPTQVPKRNMYPTDELTKNPVNYQKAVDEQFGGADDVNKT
                     PWWLQ"
     gene            19726..20535
                     /locus_tag="PLO70_05145"
     CDS             19726..20535
                     /locus_tag="PLO70_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQZ73876.1"
                     /translation="MTNSGRNIMYVLLFFSTLFLQKEVASQTKFTKLVWADEFNYKGL
                     PDSSKWNYDIGGNGWGNNELQYYTANDTLNAIVVEGVLKITARRQVKENRQYTSARLQ
                     TKNKASFKYGKIEVRAKLPAGRGTWPAIWMLGENIGKEGWPRCGEIDIMEHVGYEKDS
                     IFGTIHSEAYNHMKGTQKGKGIFIATPYDQFHTYSIEWTPDKIDFMVDGVIYNHFINE
                     HLTTKEWPFDQPFYLILNLAVGGNWGGKKGVDESVFPATLEVDYVRVFQTR"
     gene            20642..22510
                     /locus_tag="PLO70_05150"
     CDS             20642..22510
                     /locus_tag="PLO70_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQZ73877.1"
                     /translation="MVLQRNGLIPIWGWADANEKIEVRFNNQVKSVKAGKNGKWLIRL
                     DAENAGGPYDMTIKGKNTVQIRNILVGEVWLCSGQSNMEWTVGQSMNAKQEIAAANNL
                     FIRHIKIQRDISSLPQNDINAGDWQICDSTTVANFTGVGYFFAKNLYNELKIPVGLIN
                     SSWGGTNIETWISREAFENSDEFKEMIAGMPKIDLDSLSKLKVKGSVMRIEALQGTKM
                     EGMNTSQFRELSFDDSKWPVLNQPQLWEEQSIGELDGIVWLRKTIVLTSLDLTKEATL
                     VLSKIDDNDITYVNGIKVGSTNQWDANRRYTIPPGVLKEGKNVIAVRVEDTGGGGGIY
                     GDAADVKLILYNASLPLSGEWKFQVESVIKTTNQNSLPSLCFNAMISPLIPFAFQGVL
                     WYQGESNAGRSYQYRKAFPLLINDWRQKWNNSSMPFYFVQLATFNNPGNSNEGCGWAE
                     LREAQTMTLSLPNTGMCVTTDLVTNPKDIHPTNKQDVGKRLAALALNNLYSKNMICNG
                     PIYKSMEIKNDQVILSFDNIGTGLYTPDKYGYIRGFEMAGKDQVFHYAKAFIKGNTVV
                     LFNEKVENPVAVHFGWIGDASDCNLFNKEGYPAVPFRTDEWKTVTKDEKYKIEKLN"
     gene            22516..23703
                     /locus_tag="PLO70_05155"
     CDS             22516..23703
                     /locus_tag="PLO70_05155"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HQZ73878.1"
                     /translation="MRSITFFGLLFFSIVLNAQKFDGLAKTPPMGWNSWNKFACNVDE
                     KMIREMADAMVSSGMKEVGYEYIVIDDCWQVSRDENGNIVPDPIRFSSGMKALADYIH
                     SKGLKFGLYSCAGSKTCEGRPGGRGHEYQDAKMYASWGIDYLKYDFCNTDAQKAEGAY
                     KTMRDALYAAKRPIVFSICEWGSTEPWTWAKNVGHLWRTTSDIMNCWDCKVNWGGIGW
                     TLIMDKNEPLGKYAGPGGWNDPDMLQVGNGVLTDVESRSHFTMWSMMAAPLILGNDIR
                     SMTPAIKEILTNKEIIALNQDTLGKQGYRWWTLDGNIELWLKPLANNEIALCFFNRSE
                     VVKTIDFDWNRYFSHLNGKTYGVTDKTNIRDLWLKKNIGSTKNNLKATIQPHDVLTIR
                     LGK"
     gene            23759..26419
                     /locus_tag="PLO70_05160"
     CDS             23759..26419
                     /locus_tag="PLO70_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQZ73879.1"
                     /translation="MNSFSFFLRCIFPLLIILTTPILGQSQIVALPKECKFKVADDMS
                     WASPSLNDNGWESRLLGTSRQDTSGKRDLFVWYRIKVIVPSSLKKPGELGKGLRLNLG
                     KIDDVDFTFFNGKLVGQTGSLPPEYISKFQADRVYNIPLDIVEWDKENVIAVRVFSPD
                     PWVGMYEGPYKLGPLQWSDSIAVEQSITQTQNSSFGTTVKLRNNGNNPFEGTIRYSIT
                     DTKNNELFTETKPIKIQPMKGAENEAVFSEYKPVNNNIFKVGYTITENATGIAVEKYQ
                     LYLSDRQLKIIVEKEPVPVIQNKIKDAFTSIRFQDQQQQGYLGKRLNKNLAERLLKLD
                     EVGTLDGYLERPGHHPWAGEHIGKYLETASNVWRNTGDERLKMQMDRLMYQLVHAQLP
                     DGYLGTYTPEDYWTSWDVWSHKYNLYGLLGYYKATSYQPALDACKKIGDLLCKTFGNK
                     PGQLDIILAGTHIGMASTSVLDPMVELYKYTGDKKYLDFCYYILEAWEQKNGPKIISA
                     LLTKGKVTAVGNGKAYEMLSTFVGLANLYRTTGDVAMLKPLFIAWNDIVKNQLYVTGT
                     TSSHEYFQADDYLPAGRDANMGEGCVTTTWIQFNQQLFEITGDLKYFDQIELSVYNHL
                     LAAENPQTGCVSYYTPLMGAKPYTCYITCCQSSVPRGIAMVPYFTFGNIKEVPTVMMY
                     EPATYKETITTKNKKTVRLSFKVESNFPESGKATISVSPSENATFPVTLRVPTWCQSF
                     TAVINGKSYKGTSKQLIVINRSWKAGDKIKVLFQIPFKMLSGGKSYPNQMAIQRGPQV
                     LALDSTLNKEVLRIFSLNADKGVVIDKPAGANAPGLLPAGWIGQQAYPLSIAGIKNGD
                     KKQQFVLVPFADASQTGGSMKVWMPLILKK"
     gene            26423..28063
                     /locus_tag="PLO70_05165"
     CDS             26423..28063
                     /locus_tag="PLO70_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQZ73880.1"
                     /translation="MKKTGILLLCIAAGYFSIAQQQEKSNLSFLHTAGQDIINESGRK
                     IFLRGVGLGNWLLPEGYMWKFGALGDRPRKIEKVVADLIGKEKAAQFWKAFRENYITE
                     ADIRRIAELGFNSVRPALNSRLFLTEGNTPVYVEEGFQLLDSLITWCKKHKLYVIIDM
                     HGAPGGQTGANIDDSPNDIPELFIEKKYQDQLVALWVKIADKYKDEPTVAAYDLLNEP
                     LPKATGSAEKYKHLLVPLYKRLTAEIRKIDQRHMITLEGFDWSNDWSLFDKPFDNNVF
                     YQFHYYCWARPDNLNSIDEYLKRRNELNTPVWVGETGEKGNAIYWATTQLFEANNIGF
                     SFWPWKKMDTQNTPYSIKKPAGWDLIAEYTRNGAKPDAIIAEKAFNELLDNIKISNCD
                     YFEDVCNAILTRIPAKIEAENYGFSGFNRSYFVLDTANKSKFYRKNEPVQIKLDSKDK
                     EQFWSEQSIELQQSEWVVYNFESLYRKKHQLTIRASAVIAPASLTIWINNKKTVVNVI
                     GKDLLEQAIGNYKLNKGDNKIKIQVKSGALKLDWFRFN"
     gene            28101..29720
                     /locus_tag="PLO70_05170"
     CDS             28101..29720
                     /locus_tag="PLO70_05170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQZ73881.1"
                     /translation="MKKSVLSIVVFIGMTCITIAQSPNKMLAISMQTLKDKIAGGWAG
                     KMIGVTYGAPTEFNYLQRINNDSIKWTPADIKGSVWQDDIYVQLTFLMAMDKYGMEAP
                     AVKYQEMLAKAGYHLWCANMQARKNYFDGIYPPMSGNPEYNYRADDIDFQIEADYIGF
                     MNPGMIQNIINECDKIGHIMNYGDGIYGGIFLSALYSAAFFENDIQKVIETGLSSIPA
                     ESDYARIVRDVMKLHRHYPTDWKAAWRELDAKWGDVDISGAGSAFNIDAKLNGAFIVM
                     GLLYGDLDDYKTLEITTRCGADSDCNPSNALAVIGVVKGFSNLPPEMQKGVKDVGDSV
                     FINTTYSFNTAVEATYNYALKLILQNGGKVSGDSIIWPVQQFKPAKFESSFPSVVFDN
                     KVSIFSKEKWKFNGNWNVFKVKSWDGKTETEQAMFSKSRGDEIEFEFNGTGISLMGNW
                     VKDGGKADIYLDGKYHRSIDTYYNYSKQEHRNISIWHVFQLKPGKHSVKVVVKGEKNK
                     ESQDSNIYVTEAIIYRTGAKKSDLYKFSFEK"
     gene            29754..30695
                     /gene="rbsK"
                     /locus_tag="PLO70_05175"
     CDS             29754..30695
                     /gene="rbsK"
                     /locus_tag="PLO70_05175"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQZ73882.1"
                     /translation="MRVNKILVIGSSNTDMVVKTQKLPLPGETMLGGTFLMNAGGKGA
                     NQAVAAARLGGDVTLVAKVGNDIFGKQSIEGFKKENIHTDFVFITDKAPSGTAIIMVN
                     EEGENCIVVAPGANALLLPVDIAKVKKLSEAEIILMQLEIPMDTIEAVAKIAKANHQK
                     LIINPAPAQKLSDELLKGIFLVTPNETEAAMLTGIPVIDERSASEAAEVFLRKGVQNV
                     VITLGKKGAYFQNADLKFQIEAPVVQAKDTTAAGDTFNGALTVAITEKMEWEQAIHFA
                     VRAASVSVTRMGAQASVPLRSEIEPLWNHNKNHIVSL"
     gene            30724..32520
                     /locus_tag="PLO70_05180"
     CDS             30724..32520
                     /locus_tag="PLO70_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQZ73883.1"
                     /translation="MKQMLFQLLVLVLVLLTISSLSVYSQPKSNRISERTVADTNLIL
                     PPSWAFGILWGGYTNQQETIDRIGQIGKHNYPIDAYWIDSWFWSYADKGRGPKKYIDF
                     VADTSAFPDRSQMWDFMKKNNIKGGFWTWDCILETGNEKAFNDFKSKGYFSRIYDETN
                     TWHNNSISTAMFQDKQEKKNSTPCGDIDFDNPDAVSYFKKKMKHFFDEGADFIKLDRT
                     AKISTSKAMFEMSQEFGRETKGRGFLLAHSFGTEDEEYKRYPAKWTDDTRSDWTIEKP
                     IVKFADWTPPVAFKENITMFTDPAKASSKIPFLANDLGGFAVGNASKPEEELYIRWLQ
                     FSMFNPIVEVFCEPENTTSNLPWMYSERADSIFRQYAHWRMELFPYIYSYALRSRIEG
                     KHMVGKFPEHIYQYTFGDEMLVAPVYERKSTTQKVFLPDGKWINYWTNEQMQGNAEYT
                     VPAPINQIPLFIKQGSIIPMREYASSVEKGNNNSLTLHIYPGDNSRFNLLEDDGTSND
                     YLRGIYASTNIELISSLDKLALKIYPVDGYYKGMYKTRKWNLYIHCYKAPVQVKINQQ
                     NHKFSYDETKKVAIVEVPRRSVKQLIEFEIVY"
     gene            32566..>33238
                     /locus_tag="PLO70_05185"
     CDS             32566..>33238
                     /locus_tag="PLO70_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HQZ73884.1"
                     /translation="MFIVEDYGLAIMLCIVTMLCWGSWANTTKLTTKAWRFELFYWDY
                     GFGILITTLLLAFTLGSIGPEGRAFLDDIKQAGASNILSAFAGGIIFNLANILLVAAI
                     AIAGMSVAFPVGIGIALVLGVVVNYISNPQGNPLLIFGGVALIALAILLNARAYQKLQ
                     TNSATGVSKKGLILSVVSGCLMGLFYKYVAGSMVTNFNVPDAGKLTPYTALVIFAIGI
                     VISSFL"
BASE COUNT        10537 a         5635 c         7555 g         9411 t
ORIGIN      
        1 aaattaccga ctataataaa gacggatacc tggtggtaaa gaatttcctg gatgcaaatg
       61 agataaacaa gcttcagcaa atagcttttg atgatggagc catgcgtaag catgcttttg
      121 acctgaatga ccaggcagga aaaaaaacaa aactcacact ttggtacaag ccaggtaatg
      181 atgcatacgg cttattgacc aggagcgatc gaatggttaa cggtgctgac agcctgatgg
      241 aaggagaaag tgctgtttgt catttccaca gcaagctgat gcagaaagaa ccaaaagtag
      301 gaggtgcatg ggagtggcac caggattatg gttattggta taaaaatgaa ttcctgttac
      361 ctggtcaaat gatctctatt atgattgcga ttacagacgc cggcaaagaa aacggttgtc
      421 tgcaggtgat caggggctcc cataaaatgg gtcgggtgga acatggtttt gcaggagaac
      481 aggtgggagc tgcacagcgt tatgttgatc ttgctttgaa aacaatggag ttggtctatg
      541 tagaattaaa agccggggat gcattattct ttcatcccaa tatacttcat cgttcagaag
      601 ccaatttatc agacaatgcc aggtggtcat tgatctctgt ttataacagg agcagtaatg
      661 ttccatacaa tgagccatcg cagtcaagca cggttcctgt tgaaccagtg cctgatgagg
      721 cattattgaa ttggaaagag gaattgattg atgattcagc caatttcctg gaaaaagaaa
      781 aagaccaggc attaaaataa aacacatatg ttcttttcat catcacctgt atggcaaaca
      841 tccgggctta ctttgatcag gcttactttg gcaggatttc ttatctacca cggatgggaa
      901 atctttaatg acacaaaaat gaatgaatac ctggcatggg ataatttcaa acaatcaaac
      961 ggaaagttac tggtgtatgc aggcaaggcc gcagaattgc ttgcaggtat cctgtttgta
     1021 ttgggattat ttacccgtat tgcttcattg cttactatcg gaacgatgtg ctatataacc
     1081 tttatgcttg gcaatggtat tatctggaat aatgaccaac atcccttctt atttgtattg
     1141 ctggcactgg tgtttttctt tactggtgca ggacgtttta gtcttgatca atatttgttc
     1201 agtaaaaaat cattgtatct ctgatgtctg acagcctgca agtacatatt aatacaaaag
     1261 gtaaagaagc caacagttat gatgctatcg tcattggcag cggtatcagt gggggttggg
     1321 cggcaaaaga attatgtgaa aaaggtttga aaacgctggt attggaaaga ggaaggcctg
     1381 ttgatcatat caaagattat ccaacagctc atttatcaaa atgggagctg ccacaccgcg
     1441 gatggattac taatgaaaca aagcagaaaa atccactgat cagtaaagcg gctggttata
     1501 gtgaggatac acagcatttt tttgtgaagg atgctgacca tccttatgtg caggaaaaac
     1561 catttgattg gattcggggt taccaggtag gaggcaaatc acttacatgg ggaagagcct
     1621 gtgcaagatg gagtgaattt gatttcactg cacccaaacg atatgggtat ggagaatcct
     1681 ttcctattgg atataaagac atcaaagatt ggtacagcca tgtagaaaaa tttattggag
     1741 tatgcggcag ttatgatggt ataccttcta tgccggatgg agatttttta actgcctatg
     1801 aacttaatgt tgtggaaaag catatgcaga aagtcttgaa ggaaaaattc aaccggcact
     1861 atgtatcggg gcgatgggca catattaccg atcccaaaca aattcatctt gaccagggcc
     1921 gggctcaatg tatgaacagg aatttatgca tgcggggttg tccgcttgga gggtatttca
     1981 gttccaattc ttcaacatta ccatgggcgg caaagacagg taatttaact attcgtccgc
     2041 atacagtggt gcattccatt atatatgatg agcaaaaaca aaaagcagtg ggtgtaagag
     2101 tgattgatgc caatacaaaa gaggctactg agttttttgc tactatcatt tttgtaaatg
     2161 cctctgctct taataccaat ttaattttgc tcaattcggt ttctgaccgt tttccaaatg
     2221 ggttgggcaa tgacagtggc ctgttgggta aatatatatg ctttcataat taccggggcg
     2281 gcatgtgggg ctatatgcaa ggatttgaag aatggtatta taaagtatcg aagccggctg
     2341 agtgtatcat cgccaacttc aggaatgtag aaaaacagga aacagatttt tatggcggct
     2401 atgtgatcta ttccggtgcc agccgggaaa aactcaatga tcattctgcg cctgaaccgg
     2461 tcggagcctc ttttaaagaa acattgagcc agcccggtcg ttggagtgta tatatgtata
     2521 tgcagggaga aacgatcgcc aaacagtcca atcatgtaag gctgagcaaa gaggaaaaag
     2581 atcaatgggg aatacccttg ctgatcactt cggttgatta tgatgataac gatgataaaa
     2641 tggtaaaaga ctttttggaa caaggtaaag ccatgatgga ggcagcgggt ataaaagaag
     2701 tgaacacctg ggataccaaa cagcctcccg gtcttgatat tcacgaaatg ggcggtgtac
     2761 ggatgggcag caatgctaaa acttctctgc tcaatgattg gaaccaattg catcattgta
     2821 agaatgtatt tataaccgat ggcgcctgca tgacgagtac cggcagtcag agcccttcta
     2881 ttttatatat ggcatttacc gcaagggcag tagatcatgc ggtaagtgaa ttaaaaagga
     2941 gaaatttata aaatgaaacg tttagtaaca gggatagtac tggtaatgag tttgtgcaca
     3001 acacatgcac agcaaaaaac ctattgcaac ccgatcaata tcgattatgg gtatacaccc
     3061 attcctaatt tcactgaatg ggggaggcac cgggccaccg ctgatccggt gatcgttacc
     3121 tataaagggg attattattt gttcagcact aatcagtggg gctattggta cagcagtgat
     3181 atgctgaact ggaaattcaa tgcaagaaaa tttttgcggc cctggaatga agggtatgat
     3241 gaactttgcg ccccggcagt tgggatcata ggagatacaa tgattgtttt tggttctact
     3301 tatactagca agttcactat atggatgagc actaatccta aagctaatga atggaaacca
     3361 ttggtagatt catttgatat cggcggttgg gatccgtctt tctttactga cgatgatggt
     3421 aaattgtata tgtacaatgg cagtagtaat aagtttccga tgtatggaat agagttaaat
     3481 agaaaaacga tgaagccggt cggaacaagg aaagaaatgt atttgctgga accctggcgt
     3541 tttggctggc agcgtttcgg tgagcatatg gataatactt ttttggatcc ttttatagaa
     3601 ggttcacata tgacaaagca caatggtaaa tattatttgc agtatggtgc tccgggtaca
     3661 gaattcagcg gttatgcgga tgggttatta gttggtgata atccactggg accatttgaa
     3721 gcacagagtg atccggtgag tatcaagttg ggtggttttg tacggggggc cgggcatggc
     3781 gctactttcc gggataacta tatgaactac tggcatattt ccactactgt cctttcagtt
     3841 aaaaacacat ttgaaagaag attaggaata tggccggcag gttttgatgc agagggaaaa
     3901 atgttttgta acactacgtt tggcgattac ccgcattata tacctgcacc tgggttttcc
     3961 ggaatagcgt atggggtaaa tggtaaatcg gcttatttca ccggctggat gctgctgaat
     4021 tataataaac cagtacaggt ttcatctact ttaggcgggt atcttcccaa taatgcagtg
     4081 gatgaactgg ctaaaacata ctggagtgcc gaatcaggta acaaaggaga atggattcaa
     4141 accgaccttg gaaatatatc aactgtaaat gctgttcaga taaactatgc cgaccaggat
     4201 gtagaaagca acaggttggg taaatgggac aaccagtttc accagtacaa actgtattat
     4261 tctgtggatg gaaagaaatg ggttgtgtta tttgataaga gtaataacaa aacagatgta
     4321 ccgcatgatt atgtggaatt agagaaacca atacaggcaa gatttattaa actggaaaat
     4381 attcatatgc ctgccggaaa atttgccatc agtggattca gggtatttgg taatggcaac
     4441 ggtgccaaac ctgatccggt acagggattc atcgtgttac gaacggagaa agataaacgc
     4501 agtgctttta ttaaatggaa accggtggat aacgcctttg cctataatat ttattacgga
     4561 acgcatccgg ataaattata taccagcatc atggttcatt ccaataatga atactggatg
     4621 aaggccatgg atttacaaaa gacctatttt tattgtatag aatcaattaa tgaaaatgga
     4681 accagtgaaa gaagtaaggt cattaaagtt gattgacatg atgaaaaagg taataaacct
     4741 ggttattgtt tttttcctgc tgactttatc agttcagtca caggaaaaga tatcgttctg
     4801 gaatgaggtg caggctttca ggcagaaaga tagtgtgcag tttcctgcag cggggcagtt
     4861 gttatttatt ggtagttctt catttacgat gtggaaagat gtacaggatt attttcctgg
     4921 ttataagata ctgaacaggg cttttggagg ttctacgctg gttgacctga tccggtttcg
     4981 gtatgacgtt atctacccgt atcagcccaa acaaataata atttattgcg gggaaaatga
     5041 ctttgccgcc agtgatacag tgacagtaga aatggtaatg cagcggttca aaactttata
     5101 tcgtttgatc cgtgctaaat ataccagcgt acctttagta tatgtttcta tgaaaccaag
     5161 cccgagccgg gtacatctga tgtccaggta tgccgaggct aacaggcaga ttgaaatatt
     5221 tttaaataag gataaaaaag caatttttgc ggatgtatat tcaaaaatgc tgaaagaaaa
     5281 cggcagcccg atggatgata tttttttaac ggatagcctg catatgaatg caaaaggtta
     5341 tgcgatctgg gaaaaagtac tgggtaagtt tttgatcaaa taaataatga ctatggtgcg
     5401 gctaagaaaa tttgtatttc cactcttgtt actatcagtt tgcgggatga gttgtaaaca
     5461 ggaaacagct gttactaaga tcgcctttgg ttcctgcggt caccaaagcg acccacaacc
     5521 ggttttggca attgccgcag agcaaaatcc cgatgcgttc attttcctcg gggataatat
     5581 ttatggcgac acagacaata tggatacact gcggaacaaa tacgaacggc tgggtgcaaa
     5641 acctgagtac cggcaattag ccgcagcgac caaaattttt gctacctggg atgaccatga
     5701 ttttggacgc aatgatgcag gaaaatatta cccgtttaaa aaagaatcca aagagatatt
     5761 catggaattc tttaaagagc cggctaacag tgatcgcaga aagcatgatg gtatttataa
     5821 agcagaatac ctgcaagagg gtgataagat tattcagctg atcctgctgg atgttcgcag
     5881 tttcagaagt gatttactgt tatttgacaa agaaaacaag gaattacaag ccaggtattt
     5941 ttatccgctg gattatcaac cgcacactgt acccgatagt actttgctgg gagatgacca
     6001 atggaaatgg ttggaggaag aattgaaaaa gccggctgac ctgcgtttga tctgcagtgg
     6061 ttcacaattc gggattgaat ttaatggtta tgaggcctgg gctaatttcc cacttgagca
     6121 aaaacgtatg ctggaattaa ttaaaagcac aaaggcaaac ggagtgctgt tcctttcagg
     6181 tgatgtgcat tatgcagaaa tttccaggtt ggaaacagat ggattgtatc cgatatatga
     6241 tataacatca agtggtatta cttctacctg gagctttgcc actcctaata aaaacaggat
     6301 cgaagggccg gtgatggata atcattttgg attattaacc attgaatggg aaaaggatcc
     6361 tgtgatcagg atggagatca tcgataagaa taagaacaac cggattgaat acactataaa
     6421 caaaagccag attcagttca aataagcttt cagtaataat taaaaattaa cagatgaaaa
     6481 gagcagtttt aatcttgatt gttatcagtg ccttacttgc ggggaaagaa gttacaggtc
     6541 agacaactaa caatgccaaa atgaaatctt ttattgatgc attgatgaaa aaaatgaccc
     6601 tggaagaaaa gataggccag ttgaatttac ctggtagtgg tgatattgtt accggtcagg
     6661 cacagagttc tgacatcgcc aaaaagatca aagaaggaaa agtaggcggc ctgtttaata
     6721 taaagtcagt ggctaagatc cgggatgtac agaaagtagc ggttgagcaa agccgcttaa
     6781 agatcccgat gatcttcggt atggatgtaa ttcatggtta tgaaacagta tttccaattc
     6841 cattgggact gagctgtacc tgggatatga atttaattga gcgtagtgcc cggattgctg
     6901 cgatagaagc aagtgccgat ggtatcaact ggacattttc gccaatggta gatattgccc
     6961 gtgatgcccg ctggggtaga attgccgaag gaagtggtga agatgcgtat ctcggttctc
     7021 aaattgctaa agccatggta aaaggttacc agggaaacga cctgtcaaaa aataatacga
     7081 tcatggcttg tgtaaagcat tatgcattgt atggagcagc agaggcaggc cgtgattata
     7141 atacaacaga tatgagccac cagcggatgt ataatgaata cttcccgcct tacaaagctg
     7201 ctgttgaggc aggtgccgga agtgtaatgg catctttcaa tgaagtggat ggtattcccg
     7261 caacggcgaa taaatggctg atgacagatg tactgcgtaa acaatggggt tttaaagggt
     7321 ttgtcgtaac agattatacc ggtattaatg aaatgatcga tcatggtatt ggtgatttac
     7381 aaacagcttc tgcaagggct ttgatggcag ggattgatat ggacatggta ggagaaggaa
     7441 ttctcctaac gacgcagaaa tctctgaaag aagggaaagt aacattggca cagatcaatg
     7501 ctgcctgcag gagaatatta gaagcaaagt atatgctggg gttatttgat gatccatata
     7561 aatattgtga tgagaacagg gctaaaacag aaatatttac ggcagctcac cgtaaggagg
     7621 ccaggagtac tgctgcacaa agttttgtgt tgctaaagaa tcagaacaat gtattgcctt
     7681 tgaaaaaggc aggaaccatc gctttgatcg ggccattggc agataacaaa gaaaatatgc
     7741 ccggtacctg gagtgtggcg gctaattttg ataaagcgac atcagtatta actggtttaa
     7801 aagaagtggt gggtgataaa gtgaagatat tacatgcaag aggttccaac cttgatgagg
     7861 acagtttatt cgaagagaga gccggtatgt ttggtaaatc attacgtcgt gacagtcgcc
     7921 cggctaatgt tatcctgcag gaagcattgg acatcgctaa tcagtcagat gtaatagtag
     7981 ctgctttagg cgaatcatct gaaatgagtg gagaatcatc cagccgatct gatattcaaa
     8041 taccaaaact gcaacaggat ttattaaaag cattattgaa gacgggcaaa cctgtggtat
     8101 tggtattatt taccggccgg ccactggcat tgaaatggga aaatgaaaat gtgcctgcca
     8161 tattgaacgt atggtttggt ggcagcgaag caggatatgc tattgctgat gttttgttcg
     8221 gggatgttaa tccttccgga aagcttagca ctactttccc gcagaatgta ggacaggttc
     8281 ctatcttcta taatcataag aacacaggcc gccctttgga ggaaggaaaa tggttctcta
     8341 agttccgttc taattacctg gatgtaagta atgatccggt ttatcctttt ggttacggat
     8401 taagctatac cagtttcagt tacagtgata tcaaattaag cagtgcttca ttaaagggaa
     8461 accagtcatt aactgcgtca gttactgtta ccaactcagg taaatatgat gggaaagaag
     8521 tggtgcaatt gtatatccgt gatatggtag gctcggtaac gaggccggta aaagagctaa
     8581 agggtttcca gaaaattgac ctgaaagccg gcgagaccaa aacggttaca tttaatatta
     8641 ctccaaatga tcttaagttt tataattacg atttgaagtt tgactgggag cccggcgaat
     8701 ttcagattat gatcggtggt aattcaagag atgtgaaagt gggtaaagta aactggataa
     8761 aataagcaaa gggccggatg ttttaattta aaatgaaagt cagcgcaaat ggaaaaaacg
     8821 agaatactcc tggggttatt attattgcca tttgctgttt catcgcaagg gcttattata
     8881 aataccttta accggaatac taccagtctg aatggtgaat ggaattatat tgttgatccc
     8941 tatgaaacgg ggtattataa ttttcattct gagatctatg atcagaaaaa tgcaggttct
     9001 ccgtcagcct tttataataa ttaccatgca aaaaataaag cagagctggt tgaatatgat
     9061 tttgataaat caccggttat ggtaattccg ggtgattgga atacacaaaa agaaaatctc
     9121 ttttactacg aaggcactgt ctggttcaag cggtcttttg attatgcact caaagaaaaa
     9181 aacaggttat ttgtgtattt cggtgcaatc aattacaagg ctgatgtgta cctgaatgga
     9241 ataaaattgg gtactcatga gggaggattt acgccgttta atttcgagat tactgctgtt
     9301 gtaaaacctg ctggtaatta cctggtagta aaagtagata acaaacgatt cagggaggca
     9361 gtgccaacga tgaatacaga ttggtggaat tacgggggca ttacaaggga tgtgatacta
     9421 atagaagaac cacctgtatt tgttcaggat tatgcgattc aactaaagaa gggaactcaa
     9481 aacctgattg ccgggtatgt gaaattaaat gggctaactg gtggagaaaa tgttacgatc
     9541 agtataccgg agttaaaaat cgaaaagaat atcactgtat caaataatgg gtatacccca
     9601 tttgagatca ggactgataa gctgcagtac tggtctccgt cgaatccaaa actctatgaa
     9661 gtttctataa aaacatcccg gcagacgcta aaggataata ttgggtttag aacagttgag
     9721 acaaaaggag cagatatcct gatcaatggt atgcctgtct ttttaaaagg catctgtatg
     9781 catgaagaat ataaaggcag acgggcaaac agtgaagctg atgcacgggc aatgttgacc
     9841 tgggccaaag aactgggttg taattttatc aggcttgcac attatccgca taatgaatac
     9901 atgctgaaaa tggcagataa gatggggttg atggtctggg aagagatccc tgtttactgg
     9961 accattgatt ttggaaacga atcgacttat aataacgcaa agaatcagct gaccgaagtt
    10021 attacgagag ataaaaacag ggcatgtgtt atcatctggt cgatggcaaa tgaaacaccg
    10081 ctttctgaca agagaaatta tttcctaaag aatctcgtaa atcatgccag gtcacttgac
    10141 tcaacaagat tgatcagcgc agcacttttg accagggggg aaaagggagt tggaattatt
    10201 gacgataaaa ttggggagta cctggatata gttgctttca atcaatacag gggatggtat
    10261 ggcggtgatc tggcaactgc accggatgca aaatgggtaa caccttataa taagcctgtt
    10321 gttgtatctg aattcggggg ggatgccaaa caaggattgc atggtgacag ggaagaacgc
    10381 tggacagagg aataccagga atatctgtat cagcaaaacc tgctgatgat tgaaaagatg
    10441 ccaaacatcc gggggataag cccctggata ttgactgact tccggtcgcc acgaaggatg
    10501 ttaccgggca tacaggatgg ttttaaccgg aaaggattga ttgcggacaa cgggatgaag
    10561 aaaaaagcat tctttatcct ccgggagttc tataagaaaa tatcgctgaa gtataaatag
    10621 gttcactcaa atgttcaatg aaacaactcc ttacattctt tatagcacta gtactttgct
    10681 tcaacgggtt tgcccagcgt ccatttttat tgtattcacc cgattcggca ctacggcttg
    10741 aaattattac aggaactcaa ttagcatata agctatttgc aggtacacag ttactgcaat
    10801 cgtcttcttc tgttgacctg aagctggaca atggtttgca actttctgat aagttggtag
    10861 tatcgatgta caagtacaga aaagttaatg agctggttaa cgtggcagtg ccttatcgca
    10921 ggccggtcat ttctaatcac taccagcacc tggagttgat tttcaaacag ccattctccg
    10981 ttgaatttcg tttgtacaat aatggtatgg cctaccggat aggtacccgg tttaaggatt
    11041 caattattat aaaatatgaa acggcaattt tcgatgtaga cagtcagtcc gttgtatggt
    11101 ttcctcatat tgataaacga cctgaagcgg atcgctttca taccagtttt gaaggcattt
    11161 ataaaaagga actattatca gggatacctg atagtatgct gacctatgca cccgtgactt
    11221 tatcgttgcc gaacgggtat catttgtcta ttaccgatgc caatttgtat gattatcccg
    11281 gaatgttttt acaaacggca aattcagggt tacaaggtgt ttttgctcca tatcccctca
    11341 gggaagtgat gaccgaaggc gaattccctc aatttatggt taccgaaagg gctaattata
    11401 ttgccaaaat aaaaggtaca agaatgttgc cctggcgagc cgtactggta gcaaaaaatg
    11461 atattgatct cccggaaaat gatatgatct acctgcttgg cgatccaact actctcactg
    11521 atttttcatg gatcaggccg ggtaaaggga cagatgaatg gatcacaggg atcaatttat
    11581 ttaatgtacc atttgaagca ggtatcaata ctgctactta taaatactat attgattttg
    11641 ccaggcgatt cggctttgac caggtaatgt tggatgcggg ctggagtgat tacagggatc
    11701 tttttaagat caatcccaac ctggacatgg atgaactggt tgcttatgcg aagaaaaatg
    11761 atgttaaact gatgctatgg acactttgct ccactttaga taaacaactg gatgctgcgt
    11821 tggcacaatt caacaaatgg ggagtgtcat ccatcatgac tgattttatg gacagggatg
    11881 atcagccaat ggtaaatttt tatcatcgta ttgcaaaagc ctgtgcggag cataaaattt
    11941 ccatcatgtt tcatggagct tttccaccaa aaggatttaa ccggacatgg cccaatgcca
    12001 ttacacagga aggagtgttg ggagcagaat ggaatatctg gagtgacctg gctactcccg
    12061 agcacaatgt ggccattgcg tatacccgga tgctggctgg tccgctggat tatgaacccg
    12121 ggttattact gaatgcacaa aaagaccagt tccggcctat tggtaaaaat ccgatgagta
    12181 tcggaacacg atgtcatcaa ctggcaatgt ttgtggtata cgacagcccg cttcaaattt
    12241 tcagtggtaa tatcagccag ggattaaaag aaccagtctt tatggaattc ctcggcagta
    12301 ttccaacaac ctgggatgaa acaagaattt tgcagggaaa gatcggtgag tatattatta
    12361 cagccagaag gaaaggaaat gattggttta tagcgggact gaataatagt gtggcaaggg
    12421 aaatagattt gtcatttgat tttataacag agggagatta taaaatgatt gtctgtaaag
    12481 atggattgaa tgcacataat tatggaagtg attatgtttt gaacgaaatg ttggtaaata
    12541 aatcaactga gtacaattta aaaatggctc ccggtggagg atttctggtt aaattagttt
    12601 ctaaaacgaa gtaatggatt caaggaaaat aataaaagct attatagggg caggtattgg
    12661 tttattattt tcctgttcac cgacaagaaa aaatagcatg agtccgacat ttgctgctaa
    12721 cccggttgtt gctcatcgtg gagcctttaa gaagaacagc cttcccgaaa attctatagc
    12781 atcgttaaaa gaagcgattc ggttaggttg taccggttct gaatttgatg tgaggatgac
    12841 ggcagatgac tccctgatca ttaatcatga tccgcatttc aataaattag agattgaaaa
    12901 aacaacttat ttagagttga cgcaatttaa actgtcaaat ggtgaaaaat tgccgacact
    12961 aaccgattat atattggctg gtcttgataa aaacctgcaa acaagactgg tattggagat
    13021 caagccatca ggtgtcagca aggaaagggg taagcagatt gcagaaaagg tagtaaagct
    13081 ggttaaagaa ctgaatgctg gtgccccgat agtctatatc agctttgatt atgacatctt
    13141 gaaaagggta attgagcttg atccaaaagc gtcaactcaa tatcttaatg gtgataaatc
    13201 tcctgcccag ttaaaagcag atcgtattga cggggcagac tatcatttca ccgtttttaa
    13261 aaaaaatcct gactggattg aacaggcaaa aaagaataag atcatattaa atgcctggac
    13321 agtgaatgat gccgcgacca tggactggct attggccaat gggtttgatt ttattaccac
    13381 aaatgagccg gagttacttt ttgaaagagc aaaactgtca ccaataatct caggcaatta
    13441 attgatttac gcagcagtta gcaggaatta accagggcct atttttttta ccgaatccct
    13501 gattatcaac tttgaattaa gcactacatt tttgggcttt attctggtgc ttttgccaaa
    13561 ctggttaaag agcattttaa gcgctacatc gccgatgttt tcattcatat gcgaaactgt
    13621 tgtcagcggt gtatataata aatctgaata ctcctgttca tcaaaggaaa taattgataa
    13681 gtcatttggt atttgtactc cttcttcttt taatgccttt aatatcccta aagtaatttg
    13741 gtttcccatg gaaatgatag cagtaatttt actttttgca agatgtttaa ttaatttttt
    13801 tgcacaagtg taaccattgt tgaaaccaaa atcttcgccc atcaccaggg atgggtcaaa
    13861 gtggatttta ttatttttta atgcttgctt gtaaccattt acccgttggt tatttggtga
    13921 ggtgcctaca atcccctgga aacaggcaat ttttttatgc ccgttattta ttaaatactg
    13981 ggtagcttcc agcgaccctt caaaatcatt agaagaaatg tatggtaaat tcacaccttc
    14041 aaagaaccgg tcaatcaaaa ccaccggggt accgcttttg gaagcttttt gtaaatgttc
    14101 tgattccagg ccaatggggg ctattattat tccgtccacc atccagctct gcagtaaggc
    14161 aatagatttt ttttcaactt taatgtcatc attactatta caaaggaaga tattataatg
    14221 gtgcttcctg gcttcttttt ctattcctaa tgcaagctgg gcaaaccagg tattggtgat
    14281 atccggtaag attagcccaa tggtagatga tttttttaag cgaagccctt ttgcaatatt
    14341 gttgggtctg tagtttaatt tatccgctgt agttgttact aattcaattg tttttttact
    14401 gatcctgaat tgctctccca ggccattcaa aacccttgag atggttgtta cagaaacacc
    14461 cagggttttt gccatcgatt gtatggtaac gttatttttt ttcattccga aagtgatccg
    14521 ttttgcaaaa aggaagggaa tgggtagtca taaaagtagt ggttaggagg cttaaaatga
    14581 gtttttgtta aagaaatggc aaatttattt tggcgaaatt aaataaagtt ctaacttgct
    14641 caaacgattg tgcaaaatgc tcaaacgttt gagcattttg cacaatcgaa agacttattt
    14701 ttttaaacgc aaaacgactg catatgaaaa aaatcgtaca aggaggttcc cttttctttc
    14761 ttttactaat ttcatcattg gcctggtctc aaaacaaaac agttacaggc agaattattg
    14821 gagaaaatgg aattggagtt tccaaagcat cagtattggt tcagggcacc cgaatcggtg
    14881 tcacaactaa tgaaaggggc gagtttacaa tttctgtccc ttcttcagct accatacttg
    14941 ttattagttc cgttgggtac ggcacagtgg aagaacctat taacggaaga actgcaattg
    15001 agattacact taagcctggt gtacaggaaa tgcaggaggt tgttgttaca gccttaggat
    15061 taaaaagaga ggagaaggca ctgggatatg ctgttcaaaa ggtgtccgga gaaagtttac
    15121 agcgagttgc tggtttggat gttgctacat cccttaccgg taaagtagca ggggtattgg
    15181 ttagaaactc accagatttc gcagctgttc cccttgttac agttcgtgga gaaaacgctt
    15241 tattggttat cgatggtata gcttatcaaa ataaaacttt aactgatatt tccgctgaag
    15301 atattgaatc tgtaagtgtt ttgaaaggcg caactgcttc ggctttgtat ggttttcgtg
    15361 gtgcaaacgg tgccattttg attaccacca aaaacggcag ttcgaataaa gcaggagtaa
    15421 ctatagatta tacaaccaat acaatgttca ctgcgggttt cctggctatc cctgagaaac
    15481 aagctgtgta tggtcgtgga ggtagtggta tttatgccct caactcagat cagtcctggg
    15541 gtccaaaaat ggacggatct atgcaaaatc agtgggatcc ggtttcgaaa acttttacag
    15601 taaggcctta cctgcctgtt ggtaaagaca atttcaaaaa tttcctggaa caaggatatg
    15661 taaccaacaa taatttgaat attggttatc aaaaggataa aatttcactc aggaattctt
    15721 ttaactggac ggaaaataaa ggccgctatc cgaattccaa attgcaaaaa tatacttact
    15781 cctttggtgc tgaccttaac tacgataagt ttaaaatgag caccaattta tcgtatgcta
    15841 aaaaagcctc taaaaatatc gggtccaacg ggtatacttc ctatgatccg atgtatagca
    15901 tattgatctg gtcgcctgtt gattgggatc tcagtcttta caaaaataat tattggatca
    15961 agccgggtga aattcaaaac aaccattttg gattagtccc tggcggtacc attgataatt
    16021 atgccggtgt taatcaaaac aatccttact tcgaccgtta cgaaaaaaca aatgaaattt
    16081 cccgtgacat ttttaatgca gacttgacca tgagttatca attaacgact tggttaaaag
    16141 ctacagttcg ttcaggggtt gacttctaca aagaaacagg aaagttaagg tcttcatggg
    16201 gttcttatct ttcttccggt aatacagggg tccccggcgc cgattacagc ggctggaatg
    16261 gtggaagaaa ggggcagtat aatataggag taagtaatgg gtttagcaat aacaccgatt
    16321 ttatgctgac cggtgaccgg aaattcaaca agtttactgt ggaatatctt gccgggggaa
    16381 caattttcta caggcgggat gaaaatatga atgcaagtac tcaaggtggt atttccgttc
    16441 ccggtttctt ctcgatcaaa gcgtctgtta atcctgccca ggtatcacag tctacatacg
    16501 cacagcaggt aaattctgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    16621 acagcagtta ttctatcagt gctgcaacct ggggtgcatt gaatggtgct ggggttcccg
    16681 gcaatttata ctcaccaacc attgcgccaa caagttctga aacatacgag cagggtttac
    16741 aggctatgtt cctgaaaaac cgtatcatgc tggatgtgtc acactacaca aaaagagttt
    16801 tcgaccgtat tactacagtt gggttaacgg gagcttctgg ttatggtggc ctcgttacta
    16861 attttggtga agaaataaca agacgaggat gggaagtaat tcttaatggc tctcctatta
    16921 aaaataaaga ctggcaattg gatttaggta ttaactggtc aacgtttgcc agttactata
    16981 ctaagttaga ccctgtctat tcagttaaca agccatgggt aaaagttgga gaacgggctg
    17041 atcattttat cagcagggat tttcagcgtg tcccttccgg aaagggtgac caatcaggag
    17101 ctcttataca taatagcggc ggaaggccaa tcgttcacgg atatgatgta ttatttggct
    17161 actacgatcc aaaatttatc tggggtacca atgccaattt aaggtataag aatttaagat
    17221 tcttcctgtc ttttgatggt gtaaatggcg gactggcaaa cacaagaacg gaaagttata
    17281 tgtggcagtc aggtgttcat ccaaattcac tttcaccaga aagagcgtta gatgttgcca
    17341 caccaggttc taataactac atgggccaag gtgtaatagt ggtatccggc gcagctactt
    17401 atgatgcgaa tggtaatatc ttaaccgaca cccgtgtttt tgctcctaat aatataaaaa
    17461 ctacctacaa acaatatatg atcgatttgc acaacagtag tgcatggggt ggtaatggaa
    17521 gcaaagcaga tacttattcc agaacttttt tcaaactacg tgagatttct ttatcgtaca
    17581 acgtgccggc taaactgttg cataaagtgg caaaagctgc ttccattagc ttaataggac
    17641 aaaatgtact gctgaaagca aaacagttta aatattctga tcctgacggt ggtaatgaag
    17701 attttgctga cccatcaaca aggtatctcg gctttaaaat taatttcact ttctaaaaaa
    17761 ttgtagcaat gaaaaaaata cattatatac tgcttgcaac aatttcattt accattgcaa
    17821 gttcatgtaa aaagtttgat cagttaaata ccaacccgga tgtacccaca tctgtttcac
    17881 ccgatttgct tgccacaacg gtattgaaaa gtacataccg gttttggaat ccaaatccaa
    17941 cagattgggg tactgcacag ttatggagta agcattgtac aaatcttcaa aacaatccga
    18001 atccttacca gtatttttct tcttattatc cttacggagg atttggaggg gcacagaata
    18061 taaccatact taatagaatg gttgattttg cagaaggcaa cccggcaaaa ccttcctacg
    18121 aaggattaag attatttctg aaggcctggt atggtttttc tgcaacatta gatatggggg
    18181 atgttcctta ttcagaagca ggaaaagcag aagagggaat aacgcagcct atttacgata
    18241 agcaggcaga tgtatttgtt ggtgtattga atgatttaaa agctgctgaa gaaaaatttg
    18301 ctgtaggtgt caattttacc ggggatataa tgctgggtgg aaatgcagca aaatggcgca
    18361 ggctttgtaa tgccatgcag ttaagggtgt tgcaaaccat cagtaaaaaa attacacctg
    18421 cacaaaaagc acggtttgca gaaatagttg ctgcaggtaa tttaatgacc ggtaatacgg
    18481 ataactttca attggcttat cttgataata caaatgcaac ctatcctttt tacaacgggg
    18541 aaggcatgcg ggttaatttg gccatgtcaa aactgatggt ggatgcgtta aaaaatgtga
    18601 acgaccgcag gttattttat tttggagaac cggccgctta caaaatttcc ggtggtttac
    18661 tggaaaatga ttttgctgct tatgaaggcg ccccatcaga attatctcct aacctgcttg
    18721 atcttaacag ggcagccgga aagtattctt tggtaaacaa acgttatgca tcatcagcgt
    18781 ccaggattgg cgatccttat atttacttta cttatgctga gcaatgtttc ataatagcag
    18841 aagctattga agagggttgg gtagccggca atgcacagac ctactacgag aacggtgtaa
    18901 aagctatgct tgattattac agaaccttac cgactgtaat acagacaaat cttcatggaa
    18961 tggcaattac ccaaacctat attgataatt attttacagg cgaagctgct tataaaacaa
    19021 gtggaactaa aacagaacgt ttgcagcagg tatggatgca aaggtatttt atagattttt
    19081 tccagggcgg cgttaatata tcgtttagaa attttcaacg tacaggatat cctgtctttc
    19141 cgcttgatcc ggctacttct ttaaattccg gagctccaac acaggttcct aaacgcaata
    19201 tgtacccaac tgatgagtta actaaaaacc ctgtaaacta tcaaaaggct gttgatgaac
    19261 agtttggcgg agctgatgac gtaaataaaa caccatggtg gttgcaataa acggaaaacc
    19321 aaagcgtaca caatcagcaa attttatata tgcgtgtcta agagctaaat ataagtggag
    19381 ggggcatcaa aggcctcctc cacttatttc aatatgacca cttcggttaa agtacactga
    19441 agatcacttt tatcctcttc ctgagaaatt accacctgta accagtaata atgacttgtt
    19501 acagtaaatg aaaacatcca atgtttattg gcaaaaccga caatgtacgt tagtgcagta
    19561 taatttattt aaaacggata caaagttttt catagcaagc agtttttcat aagcaataaa
    19621 gcgaaagaaa gtggatcgta ttttcttttc taagtgaata cctccacttt ttaaaacaaa
    19681 cgggagcggc ctactttaat aatcacaatg ctttaattta attcaatgac aaacagcggg
    19741 agaaatataa tgtatgtact tctttttttc agtacactgt ttcttcaaaa agaagtagca
    19801 tcacaaacaa agtttacgaa actggtctgg gcagatgaat ttaattacaa aggtttacct
    19861 gacagcagta aatggaatta tgatattggg ggaaatgggt ggggcaataa tgaattgcag
    19921 tattataccg ccaatgatac tttgaatgca attgtagtgg aaggggtgtt aaaaattact
    19981 gcaaggcgcc aggtaaaaga aaacaggcaa tatacttctg cgaggttaca gacaaagaat
    20041 aaggcttctt tcaaatatgg caagattgaa gtaagagcca aactgccggc aggaagaggt
    20101 acctggcctg cgatatggat gctgggagaa aatattggta aagaaggatg gccccgttgt
    20161 ggtgagattg atattatgga gcatgttggt tatgagaagg acagcatctt cggaacaatt
    20221 cactcagagg cttataatca catgaaaggc actcaaaaag ggaagggcat attcattgca
    20281 acaccgtacg accagtttca tacctattcg atagaatgga cgccagataa aattgatttt
    20341 atggtggatg gggtgattta taatcatttt ataaacgaac acctgaccac aaaagaatgg
    20401 cctttcgatc agccatttta cctgatcctg aatttagcag ttggcggcaa ctggggaggt
    20461 aaaaaaggtg tggatgaatc tgttttccct gccactttgg aagtggatta tgtaagagta
    20521 tttcaaacca gataataaaa aaaagatgtt taaaaaatgc tgtttattgc ttgccggtat
    20581 aattagtttc tgtttgcagg cccgggctaa ggttattctg cctaagatct tttctgataa
    20641 tatggtatta cagcgaaatg gtttgatccc catatgggga tgggctgatg caaatgaaaa
    20701 aatagaagtg aggtttaaca atcaggttaa atctgtcaaa gctggtaaaa atggcaagtg
    20761 gctgataaga ctggatgcag aaaatgcagg cggcccctat gatatgacca ttaaagggaa
    20821 aaatacagtt cagataagga acatattggt aggtgaggta tggctatgca gtggtcagtc
    20881 gaatatggaa tggacagttg gccagtctat gaatgcaaaa caggaaatag cagccgcaaa
    20941 taatcttttt atccggcata taaaaattca gcgggatatc agttcattgc cccaaaacga
    21001 tattaatgcc ggtgattggc aaatttgtga ctcaactaca gtagccaatt ttacaggggt
    21061 aggatatttt tttgccaaga atctctacaa cgaattaaaa attccggttg ggttaattaa
    21121 ttcatcctgg ggaggtacaa acattgaaac ctggataagc cgggaagctt ttgaaaatag
    21181 cgatgagttt aaagagatga ttgcgggaat gccaaagata gatttagatt ctctttctaa
    21241 attaaaagtg aaaggtagtg taatgaggat agaggctttg cagggtacaa aaatggaagg
    21301 catgaataca agccagttca gggagctttc atttgatgat tcaaaatggc ctgttttgaa
    21361 tcaaccgcaa ttatgggaag aacaaagtat cggggagctg gatggtattg tttggcttag
    21421 gaaaacaatt gtactgactt ctcttgactt aacaaaggaa gcaacgcttg tgttatcgaa
    21481 gattgatgat aatgatataa cgtatgtaaa cggaataaaa gttggaagta ctaaccagtg
    21541 ggatgcgaac agaagatata caattccacc cggagtttta aaggaaggta agaatgtaat
    21601 tgcagtaaga gtggaggata ccggcggtgg tggtggaatt tatggggatg cagcagacgt
    21661 aaaattaatt ttgtacaatg cttctctccc tttgagtggt gagtggaaat tccaggttga
    21721 gtctgtcatc aaaacaacca accaaaattc acttccctct ttatgtttta atgcaatgat
    21781 aagtccgttg atcccattcg cattccaggg agtattgtgg taccaggggg aatctaatgc
    21841 cggacgatca taccaataca gaaaagcatt tcctttattg ataaacgact ggagacagaa
    21901 atggaataat tccagtatgc ctttttattt tgtgcaactg gcaactttta ataaccccgg
    21961 taacagtaat gaaggttgtg gctgggctga actgagagaa gcacaaacaa tgaccttgtc
    22021 tttgccaaat acaggcatgt gtgttactac tgatctcgtt actaatccta aagatattca
    22081 ccctacaaat aagcaggatg ttggtaaacg cctggcggct cttgccttga acaatcttta
    22141 cagtaagaat atgatttgca atggtcccat ttataagtca atggaaatta aaaatgacca
    22201 ggtcattctt tcatttgata atatcggaac cggtttatac actcccgata aatatggtta
    22261 catcaggggt tttgaaatgg caggaaagga ccaggtgttt cattatgcca aagcatttat
    22321 caaaggcaac acagtagtac tattcaatga aaaagtagag aatccggttg ctgttcattt
    22381 tgggtggatt ggcgatgcca gtgattgcaa cctgtttaat aaagaaggat accctgctgt
    22441 gcctttcagg acagatgaat ggaagacagt tacaaaagat gaaaagtata aaattgaaaa
    22501 actaaactaa agagtatgag aagtattact ttttttggtt tgttattctt ttccatagtc
    22561 cttaatgccc aaaagttcga tggattggct aaaacgccac ctatgggttg gaacagctgg
    22621 aacaaattcg cctgcaatgt ggatgaaaaa atgattcggg aaatggctga tgcaatggtt
    22681 tccagtggta tgaaagaggt gggatacgag tacattgtca ttgatgattg ctggcaggtt
    22741 tcccgtgatg aaaatggaaa tattgttccc gacccaatac gcttttcttc cggcatgaaa
    22801 gccctggctg attatattca ctccaaagga ttaaaatttg gtttgtattc ctgtgcgggc
    22861 agcaaaacct gtgaaggccg gcccggtggc cgtggacatg aataccagga tgcaaaaatg
    22921 tatgcgtcat ggggcattga ttatctgaaa tatgattttt gtaatactga tgctcaaaag
    22981 gcagaaggag cctataaaac aatgcgggat gcattatatg ctgcaaagcg tcccatagtt
    23041 ttcagtattt gtgaatgggg aagtactgaa ccctggacat gggcaaaaaa cgtcgggcat
    23101 ctttggagaa ctaccagcga tataatgaat tgctgggact gtaaagtgaa ttggggtggt
    23161 attggctgga cattaattat ggataaaaat gagccactcg gaaaatacgc cggacccgga
    23221 ggttggaatg accctgatat gctccaggtt ggaaacggag tgttgacaga tgtagaatcc
    23281 cgttcccatt ttacaatgtg gagtatgatg gctgcgccgc tgatattggg caacgatatc
    23341 cggtcgatga caccggctat taaagagata ctgactaata aagaaatcat cgcattgaat
    23401 caggatacac tgggaaagca aggctacagg tggtggacat tggatggcaa tattgagctt
    23461 tggctcaaac ctcttgctaa caatgaaatt gcattgtgtt tttttaaccg gagtgaagtt
    23521 gtgaaaacca ttgattttga ctggaacagg tatttcagtc atctcaacgg taaaacttac
    23581 ggtgttacag acaagacgaa catccgtgat ttatggctca aaaaaaatat tggctcaaca
    23641 aaaaataatt tgaaggctac cattcagcca catgatgtat tgacaatcag attgggtaaa
    23701 taatagataa aatattttct agtaataaaa aataatttaa tcaataacag ttactggtat
    23761 gaattcattt tctttttttt tacgctgcat ttttcctctg ctgattattt tgacaactcc
    23821 aattttaggg caatcgcaaa ttgttgcatt acccaaagaa tgcaagttca aggttgctga
    23881 tgatatgtcc tgggcctctc cttcattaaa tgataacggc tgggagagca ggttattggg
    23941 tacaagcaga caggatacca gcggtaaaag ggaccttttc gtttggtatc gtattaaagt
    24001 aattgttcca tcaagtctga aaaaacccgg tgagttgggc aaagggcttc gtctgaacct
    24061 cggaaagatt gatgatgttg atttcacttt ttttaatggg aaactagttg gtcaaacagg
    24121 tagcctgcca ccagaatata taagcaaatt ccaggctgac agggtttata acattccact
    24181 ggacatagtt gaatgggata aggagaatgt tattgcagta agggttttca gtcccgatcc
    24241 ctgggtggga atgtatgaag ggccttataa gttaggtcca cttcaatggt cggattcgat
    24301 tgctgtagag caaagtatta ctcaaacaca aaacagtagt tttggcacaa ccgttaagct
    24361 tagaaataat ggtaataatc cgtttgaagg aaccatcagg tatagcatta cagatacgaa
    24421 aaataatgag ctattcactg aaacaaagcc gataaaaatt cagccaatga aaggagccga
    24481 gaatgaagca gtgttctcgg aatacaaacc tgtaaataat aatatcttca aagttggtta
    24541 tacaattaca gaaaacgcaa cagggatagc ggtagaaaaa tatcagcttt atctttctga
    24601 taggcagttg aaaattattg tagaaaaaga gcctgttccg gttattcaga acaagataaa
    24661 ggatgctttt acatctatac ggtttcagga tcagcaacaa cagggatatc ttgggaagcg
    24721 gttgaataaa aatctggcag agcgtttatt aaaactggat gaagtgggaa cattggatgg
    24781 ctacctggaa agaccgggcc atcatccctg ggcaggggag catatcggta agtatcttga
    24841 aacggccagc aatgtctgga gaaatacagg cgatgaaagg ttgaagatgc aaatggacag
    24901 gcttatgtat caattggtgc atgcgcagtt gccagatggt tatttgggca cttatactcc
    24961 cgaagactac tggacaagct gggatgtttg gagccacaaa tataacctgt atggattgct
    25021 tggatattac aaggcaacta gctatcaacc agcacttgat gcttgtaaaa aaatcggtga
    25081 tctgctctgc aagacttttg gaaataaacc cggacaactt gatatcatcc tggcaggtac
    25141 ccatataggt atggcatcta ccagtgtgct ggatccgatg gttgaattgt ataagtacac
    25201 aggggataaa aagtaccttg atttttgcta ctatatactg gaagcttggg aacaaaaaaa
    25261 tggtcctaaa ataatcagtg ctctattgac aaaaggtaaa gtaaccgctg tgggtaatgg
    25321 caaggcatat gaaatgttat ccacgtttgt ggggctggca aatttataca gaacaacggg
    25381 tgatgtagca atgttaaaac cgttatttat tgcctggaat gatattgtaa agaaccagct
    25441 ttatgtaacc ggaaccacca gttctcatga gtattttcag gcagacgatt atttacccgc
    25501 aggcagagat gccaatatgg gtgagggttg cgtaacaact acctggattc agtttaatca
    25561 gcaattgttc gaaataacag gcgacttaaa atactttgat cagattgaac tgtctgtata
    25621 taatcattta ttggctgccg aaaatcctca aacaggatgt gtaagttatt ataccccttt
    25681 gatgggcgcc aaaccttata cttgttacat cacctgctgt cagtcgagtg tgccaagagg
    25741 gattgccatg gttccttatt tcacttttgg aaatatcaaa gaggtgccaa cagtaatgat
    25801 gtatgagcct gctacctaca aggagacaat taccacaaaa aataaaaaaa ctgtacggtt
    25861 atcctttaag gttgaaagca attttcctga aagcggtaag gcaactatct ctgtaagtcc
    25921 ttcagaaaac gcaactttcc ctgtcacatt gagagtgcca acctggtgtc aatcatttac
    25981 tgcagtaatt aatggtaaat catataaggg aaccagtaaa cagcttattg taattaatcg
    26041 tagctggaaa gcaggagata aaataaaagt cttatttcaa atccctttca aaatgctgtc
    26101 gggtggaaaa agttatccta accaaatggc aattcagcgg ggaccacaag tgttggcgtt
    26161 agacagcaca ctaaataaag aggtgttaag aatattttca ttgaatgctg ataagggtgt
    26221 ggtgattgat aagccagcag gagcaaatgc tccgggtcta ttgccagcag gctggatagg
    26281 acaacaagct taccctttaa gtattgcagg tattaaaaat ggagacaaaa agcagcaatt
    26341 tgtattggtg ccttttgctg atgccagcca aaccggtggt tcaatgaaag tttggatgcc
    26401 attaatttta aaaaaataag gaatgaaaaa aaccggtatt ctgcttcttt gcatagccgc
    26461 tggctatttt tcaattgcac aacaacaaga gaaaagcaac ttaagttttt tacatactgc
    26521 tggccaggat attattaatg aatctggcag gaaaattttc ttaagaggag ttgggctggg
    26581 aaactggctt ttgcccgaag gatatatgtg gaaatttgga gctcttggcg accggccccg
    26641 caaaattgaa aaagtagttg ctgatcttat tgggaaagaa aaggcagctc aattttggaa
    26701 agcattcagg gaaaactata ttaccgaggc cgatataagg cggatagctg aattgggatt
    26761 taattctgta agacctgcat taaactcaag attgttttta acggaaggga atacccctgt
    26821 ttatgtggaa gaaggatttc agttactgga ctcccttatt acatggtgta aaaaacataa
    26881 gttgtatgtt ataattgata tgcacggagc acccggcggg caaacgggtg ctaatattga
    26941 tgatagccct aatgatatcc ccgaactttt tatagaaaaa aaataccagg atcaactggt
    27001 tgcactctgg gttaaaattg cagataagta taaagacgaa ccaactgtag ctgcgtatga
    27061 tttactaaat gagccactac ctaaggcgac aggttctgct gaaaaataca agcacttgct
    27121 ggtacccttg tataagcggt taactgctga aataagaaag atagaccaaa ggcatatgat
    27181 tacccttgag ggttttgact ggtcaaatga ctggtctctt tttgataaac catttgataa
    27241 taatgtgttt taccagttcc attattattg ctgggccaga cctgataatc tgaatagtat
    27301 agatgaatat ttaaaaagga gaaatgaatt aaacacacca gtttgggtag gcgaaaccgg
    27361 ggaaaaagga aatgcaattt attgggcaac tacacaatta tttgaagcta acaacatcgg
    27421 cttttcattt tggccctgga aaaagatgga tacacaaaac actccttatt caattaaaaa
    27481 accggctggt tgggatttaa tagcagaata tacccgtaat ggtgcaaagc cggatgccat
    27541 tattgctgaa aaagctttca atgaattact ggataatata aaaatttcca actgcgacta
    27601 ttttgaagat gtatgtaatg ctattttaac caggatccct gcaaaaattg aagcagagaa
    27661 ttatggcttt agtggtttta accgttctta ttttgtttta gatacggcga ataaatcaaa
    27721 attttacagg aaaaatgaac cggttcagat taaactggac agtaaggata aagaacaatt
    27781 ttggtcagag caatccattg aacttcagca atcagaatgg gtagtttata attttgaaag
    27841 tttatacagg aaaaaacacc aattaacaat cagggcatcg gcagttattg cacctgccag
    27901 cttaactatt tggatcaata ataagaaaac tgtagttaat gtaattggca aagacttgtt
    27961 agagcaggca atcggtaatt ataaattgaa caaaggggat aataaaataa aaatccaggt
    28021 aaaatctggt gcgctgaagc tggattggtt ccggtttaat taaaaagaac tattagttat
    28081 tgaatttaag aaaagttaaa atgaaaaaat ctgttctgtc gattgttgta tttatcggga
    28141 tgacgtgtat tacaattgcg caatcaccaa ataaaatgct cgcaataagt atgcaaacat
    28201 tgaaagataa aattgcaggt ggatgggccg ggaaaatgat cggtgttact tacggggcac
    28261 ccacagaatt caattatctg caaagaataa ataacgattc tattaaatgg acacctgcag
    28321 atattaaagg cagtgtctgg caagatgata tttatgtgca acttactttt ttgatggcta
    28381 tggataaata tggaatggaa gccccagcgg taaaatacca ggaaatgctc gcaaaagccg
    28441 gttaccacct ttggtgtgcc aatatgcagg cgagaaaaaa ttattttgat ggtatttatc
    28501 ctcctatgtc cggcaatcct gagtataatt atagggcaga tgatattgac tttcagattg
    28561 aagcagatta tataggcttt atgaatcctg gtatgataca aaatatcatt aatgaatgtg
    28621 ataagatagg acatatcatg aattatgggg atggtatata tggaggtata tttttaagtg
    28681 ctttatatag tgccgctttt tttgagaatg atattcagaa agtaattgaa acagggcttt
    28741 cttctattcc tgcagaaagt gattatgcca gaattgtcag ggatgtaatg aaacttcaca
    28801 ggcattatcc gaccgattgg aaggctgcct ggcgggagtt agatgctaaa tggggcgatg
    28861 tggatattag tggtgccggt tctgcattta atatcgatgc aaagctaaat ggggcattta
    28921 ttgttatggg acttctatat ggggatcttg atgattataa aacactggaa ataactacac
    28981 gctgtggagc agattcagac tgtaatcctt caaatgcatt ggcggtgatt ggtgtagtta
    29041 aagggtttag caacctgcca cctgaaatgc aaaaaggagt gaaagatgtt ggtgattctg
    29101 tttttattaa tactacgtat tcattcaata ccgcagtaga ggcaacttat aattatgctt
    29161 taaaattaat acttcagaat ggagggaaag taagtggcga tagcattata tggccggttc
    29221 agcaatttaa gccggcaaaa tttgaaagct cttttccttc agttgtattt gataataagg
    29281 tgtcaatttt ctcaaaagaa aaatggaaat tcaatgggaa ttggaatgta tttaaagtga
    29341 aatcctggga cggtaaaacg gaaacagaac aggccatgtt ttcaaaaagt aggggtgatg
    29401 agattgagtt tgagtttaac ggaacaggaa tttcattaat gggaaattgg gtaaaagatg
    29461 ggggcaaggc cgatatttat ttggatggaa agtaccatcg gagcatagac acttattata
    29521 attacagtaa acaggagcat agaaatatca gtatctggca tgtctttcag ttaaaaccag
    29581 gtaagcattc tgtaaaagta gttgtgaaag gagaaaagaa taaagaatca caggattcaa
    29641 atatttatgt cactgaagca attatttata gaacaggggc taagaaaagt gatttgtata
    29701 agttttcttt tgaaaaataa tgggtggcag gatataactt ttaaaaaaaa ggtatgagag
    29761 taaataaaat attagtaata gggagctcca atacagacat ggttgtaaaa acgcaaaaac
    29821 ttccattgcc cggcgaaaca atgcttgggg gcacttttct tatgaatgcg ggaggtaaag
    29881 gagctaacca ggctgttgct gctgccaggc tgggtggcga tgttacatta gttgcaaaag
    29941 tcggcaatga tatttttgga aaacaaagta tagaaggatt taagaaagaa aatatccata
    30001 ctgattttgt tttcattaca gataaggctc catccggtac tgccataatt atggtaaatg
    30061 aagagggtga aaattgcatt gttgttgctc ccggagccaa tgcgttgctt ttaccagtag
    30121 atattgcgaa ggttaaaaag ctttcagaag cggaaataat tttaatgcaa ctggaaattc
    30181 cgatggacac aattgaggca gtagccaaaa ttgcaaaggc aaatcatcag aaattaatta
    30241 ttaatcctgc gccggcacaa aaactgtctg atgaattact gaaaggaata ttcctggtca
    30301 ctcccaatga aacagaagct gcaatgctga ccggtatacc tgtaattgat gaaagaagcg
    30361 catctgaggc cgctgaagtg tttttaagaa agggagttca aaatgtggtt attacattag
    30421 ggaaaaaggg agcttatttt caaaacgctg atttgaaatt tcagattgag gcccctgttg
    30481 tgcaggcaaa ggatacaaca gctgcagggg atacattcaa cggggcatta actgttgcta
    30541 ttactgaaaa gatggaatgg gaacaggcca ttcatttcgc agtgcgggcg gcatctgtat
    30601 ctgtaaccag gatgggtgca caggcttcag ttccacttcg gagtgaaata gaacctctat
    30661 ggaatcataa taaaaatcat attgtcagct tgtaattaca ggactcacaa aaaaataatc
    30721 cggatgaaac aaatgttgtt tcaattattg gttttagtcc ttgtgctgct tacaataagt
    30781 agtttatctg tttactctca accaaagagc aacaggattt ctgaacgaac tgttgctgat
    30841 acaaatctaa tcctccctcc ctcctgggca ttcggtattt tatggggcgg ttatactaat
    30901 cagcaggaaa ctattgaccg gatcgggcaa atcgggaagc ataattaccc gatagatgcc
    30961 tattggattg attcctggtt ttggagctat gccgataagg gaagaggccc aaaaaaatat
    31021 atagattttg tagctgatac cagcgctttt cccgacagat cccaaatgtg ggactttatg
    31081 aaaaagaaca atataaaagg tggattctgg acctgggatt gtattctgga aacaggtaac
    31141 gaaaaagcat tcaatgattt taaatccaaa ggctattttt cacgtattta cgatgaaaca
    31201 aatacctggc ataataacag tattagcact gcaatgtttc aggataaaca ggaaaagaaa
    31261 aactcaactc cttgtgggga tatagatttt gataatccgg atgcagtcag ttatttcaag
    31321 aagaaaatga aacacttttt tgatgaaggt gctgatttta taaagctgga ccgtaccgca
    31381 aaaatcagta ccagtaaggc tatgtttgag atgagccagg aatttgggag agaaacaaaa
    31441 ggccgcggct ttttgctggc tcatagcttt ggaacagaag atgaggaata caaacgttat
    31501 cctgctaaat ggacagacga tacccgcagc gactggacaa ttgaaaagcc aatcgtcaaa
    31561 tttgcggatt ggacaccgcc ggtggcattt aaagaaaaca ttaccatgtt tactgatcct
    31621 gcaaaagcca gcagtaagat cccttttctt gcaaatgacc ttggtggctt cgctgtcgga
    31681 aatgcaagta agcctgaaga agaattatat atacggtggc tgcagttttc tatgtttaac
    31741 cccattgttg aagttttttg tgagccggaa aacaccacat caaatctgcc atggatgtat
    31801 tccgaaagag cagacagtat ttttagacag tatgcacatt ggaggatgga attgtttcct
    31861 tacatctaca gctatgccct ccgtagccgt attgaaggga agcatatggt gggaaaattt
    31921 ccagaacata tttaccaata tacttttgga gatgaaatgc tggtggcacc ggtttacgaa
    31981 agaaaatcaa ctactcaaaa agtattctta cccgatggca aatggataaa ttattggaca
    32041 aatgagcaaa tgcagggtaa tgctgaatat actgtaccag cacccatcaa tcaaattcct
    32101 ttatttatca aacagggttc aatcatacct atgcgtgaat atgcatcatc tgttgaaaaa
    32161 gggaataaca acagcttgac acttcatatt tatcccggcg ataatagtcg atttaatctg
    32221 ctggaagatg atggaacaag caatgactac ctcaggggca tatatgcttc aactaatatt
    32281 gaactgataa gcagtctgga caaattggct ttgaaaatat atccggtaga cgggtattat
    32341 aaaggaatgt acaagacaag aaagtggaat ctgtatatcc attgctataa agcaccagtg
    32401 caagtaaaaa taaatcagca gaaccataaa ttcagctacg atgaaactaa aaaggtagca
    32461 atagtggaag tacccaggcg gtcggtaaag caattaattg aatttgaaat tgtctattaa
    32521 aaatagcata gactttaaaa tattttatca tctaaaacta taaccatgtt tattgtagaa
    32581 gattacggat tggccattat gctttgtatt gtcaccatgc tttgctgggg ttcctgggct
    32641 aatacgacaa aacttaccac caaagcctgg cggtttgaac ttttttattg ggattatggt
    32701 tttggtatat tgatcaccac attgcttctg gcattcacat tgggaagtat tggcccagag
    32761 ggcagggctt tccttgatga tattaagcag gcaggtgcca gcaatatcct gtctgctttt
    32821 gcaggcggca ttatttttaa tttagcaaat attttattgg tggctgctat tgccattgca
    32881 ggaatgtctg ttgctttccc ggttggtatt ggcattgcat tggtattggg cgtagttgtt
    32941 aattatattt caaatcccca aggaaacccc ttgctcatat ttggcggtgt agctttaatt
    33001 gcattggcta tattattaaa tgcaagggcc taccagaagc tgcaaacaaa ttctgctacc
    33061 ggtgtttcca aaaaagggtt gatattatca gtcgtaagcg gctgtttaat gggtcttttt
    33121 tataagtatg ttgccggttc aatggttacc aatttcaatg taccggatgc cgggaagcta
    33181 actccttata ctgcccttgt aatttttgcg attggtatcg tgatcagcag ttttcttt
//
LOCUS       DAORTI010000024        32947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_906_length_32947_cov_6.411772, whole genome shotgun sequence.
ACCESSION   DAORTI010000024 DAORTI010000000
VERSION     DAORTI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32947
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_32947_cov_6.411772"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLO70_05190"
     CDS             <1..258
                     /locus_tag="PLO70_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQZ73885.1"
                     /translation="KEGKPLSLELLRDYYKEFSNLSLSQRISLHKLREDRADVIVPAL
                     LIYINVMRWADAEEIFVPKIGLADGLIHNLYEEVRLKEIEV"
     gene            288..1103
                     /gene="panB"
                     /locus_tag="PLO70_05195"
     CDS             288..1103
                     /gene="panB"
                     /locus_tag="PLO70_05195"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQZ73886.1"
                     /translation="MSVNKEVKRVTTNTLLKMKAAGEKISMLTAYDFSFAKIIDGAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHAQSVIRGVDRCLVVVDLPFGSYQSNSDI
                     ALASAIRIMKETGAHSVKMEGGQEVIDSIKKIVSAGIPVMGHLGLIPQSIYKFGTYAV
                     RAKEEVEAEKLRKDASLLQEVGCFAIVLEKIPADLAKEVSASLMIPTIGIGAGGDCDG
                     QVLVMHDMLGINTEFKPRFLRQYLNIHEQATKAVQHYISDVKSGDFPNDNEQY"
     gene            1162..2685
                     /locus_tag="PLO70_05200"
     CDS             1162..2685
                     /locus_tag="PLO70_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQZ73887.1"
                     /translation="MEFLKTLKIEEKNKGVSTGTNWINSTGETIDSFSPVDGKKIASV
                     TATDKESYEAVVKKAEEAFKTWRLVPAPKRGEIVRQIGEALRQHKEPLGKLVSYEMGK
                     SLQEGYGEVQEMIDICDFAVGLSRQLYGLTMHSERPGHRMYEQYHPLGIVGIISSFNF
                     PVAVWSWNTMLAWVCGDVCIWKPSEKVPLCSIACQNIISEVFKKNNVPEGVSGLVIGG
                     REVGEWMAADIRIPLVSATGSTAMGKAVGATVGARLGRALLELGGNNAIIISKEADLD
                     IAILGSLFGAVGTAGQRCTTTRRLIIHEEIYDTVKNKLISAYKQLRIGNPLDQKNHVG
                     PLIDKAAVSMYLDSLEKCKIEGGNFIVEGGVMSGEGYESGCYVKPCIAEAQPGFKIVQ
                     HETFAPILYLLKYNTIEEAIAIQNEVPQGLSSSIMTLNLREAEQFLSHAGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINFSNSLPLAQGIRFDV"
     gene            2799..4214
                     /locus_tag="PLO70_05205"
     CDS             2799..4214
                     /locus_tag="PLO70_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ73888.1"
                     /translation="MRKCILSFSFIISLLVTTHAQVRVAIVAGGHQSSVLEENDLPNW
                     SELKSNYTGRIGFHTGFIADIAFSPKSKLFFQPGVVYYNKGRKFSQRFDPPLGIIINQ
                     KSTQFLNYIDVPLNLVLKFGKKTKFIIGGGPYGSFFYSGKETAQTITTGGITESTENN
                     DLSVGNQPGQFRTTDYGINGLAGLEFRGFFITANYSRGLNDFYESTVYNGTFRHEVIG
                     GTLGVFLGKTEKIEKKIKDIDKDGINDDKDHCPEEPGTAATNGCPDKDADGIADKDDK
                     CPDIAGLIKYNGCPVPDTDKDGINDEEDKCPNASGFARYGGCPVPDTDGDGVNDEEDK
                     CPTVKGTKQRNGCPIEEIKKEIVEKVNFAAKRIQFQSAKAVLLPQSLKVLDEVANILK
                     DNPELSLSIEGHTSGDGIFEANMKLSDERAKNVKGYLISKGIDAARLTAKGFGPTQPI
                     NKGKTTAEKAQNRRVELKLSN"
     gene            4341..6032
                     /locus_tag="PLO70_05210"
     CDS             4341..6032
                     /locus_tag="PLO70_05210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HQZ73889.1"
                     /translation="MNRIMKRTTLLAIGGMLTSALFAQKDEVPKGWHMLDKASSGYNG
                     ISVDKAYEFVKTKKLKSNTVIVAVIDSGIDTLHEDLKPVLWKNTKEIPGNGIDDDKNG
                     YVDDIHGWNFLGGRDGRSVDKDSYEAARVYHKFKTKYEGKDVNVSSLSKEDAFEYQMW
                     KRSEKEVAGDSKSSGMELIFMKRAYTNCLKSDSILRTAMGKEKYTGKELGEFTSDDPN
                     VKKAKNTLFSLMSGNDALETTNVEFLEGFGDYVSGEEKKAEAGSTPPTPYRADIVKDN
                     YDDFNDKFYGNNDVMVSNKSALHGTHVSGIIAAARKNGKGMDGIADNVKIMSLRAVPD
                     GDEHDKDIALAIRYAVDNGARVINMSFGKGFSPEKMWVDEAVKYAESKGVLMVHAAGN
                     DAKNLDTTFNFPTPQLLDGSRPDNWITVGASGDPKAGGLTANFSNYGKKEVDVFAPGV
                     KIYATVPGGNTYQNLQGTSMASPVVAGLAAFIMEYYPNLSARQVKMVIEKSAQKPADN
                     VKNPGNDEMVNLGELSRTGGIINAYEAIKLAATIKGERKPSPVKPSTKVKAKVKG"
     gene            6152..7036
                     /locus_tag="PLO70_05215"
     CDS             6152..7036
                     /locus_tag="PLO70_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HQZ73890.1"
                     /translation="MISYNTKDWFTFITRFHKADTFRQLFPLILAISAYSGIIAWLEL
                     DYWKLSEKDYVRNIPVMHTLLGFAISMLLVFRTNTAYDRWWEGRKLWGSLVNNSRNLA
                     MKLNTILPAEDNSQRSFFRKVIPAFAYALHNHLHKEQTRIELFEEEEHKHVFQRIDKG
                     KHIPNQIALLMYQHIHQLLKEEKISGEQLLFLNSELQSFTDICGACERIKNTPIPFSY
                     SVFIKKFIFFYIMTLPFGYVFQLGFYVVPVVAFIFYVLASLELIAEEIEDPFGGDSND
                     VPTVALAQNIHRHVTEII"
     gene            7111..7467
                     /locus_tag="PLO70_05220"
     CDS             7111..7467
                     /locus_tag="PLO70_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73891.1"
                     /translation="MSKFSSFILLTILTITLLIACSKDNDTGASLDCSTVTNKAFAAD
                     INPIIQSSCAFSSGCHGSGSTNGPGVLTSYTSVFNARTAIRSAVASGSMPQGSSLATN
                     QKNSILCWIDSGAPNN"
     gene            7556..9976
                     /locus_tag="PLO70_05225"
     CDS             7556..9976
                     /locus_tag="PLO70_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQZ73892.1"
                     /translation="MRLLTVILSFIFFSCTVYAQSKLDKLTVDKIMRDPKWMGTSPSS
                     PFWSNDGQLLYFMWNPEQAPADSLHYISSLSKVPVKASVEQKQALSAQGNYIYNQSGT
                     AYVYARNGDIYFTEVKTGKSKRITQTTDNESGPRFCFNETKIVYSRNQNLYAWDIAGG
                     ETIQLTNIKTGESSSSAAGGFQRTGAQPTTRSGGNPQEQWLQNDQLTYFQVLKDRKSK
                     REMADAYNKSLPKEKELRSINLEDKTIQGLSISPDGRFVSYRLFKAATGSKTTIVPSY
                     VTETGFTTDIPSRTKVGSSQGSSEFFIYDREKDTILNIKTDSIPGIKELPDYVKDYPK
                     QLEERQKKAANRLVNFTGLTWSPKGMNAVIEIRSQDNKDRWLMQWDKNTGGLKLLDRQ
                     RDEAWIGGPGIGGFGNTNTGWIDENIFWYRSESSGYSHLYTVNITTGVKKALTSGSYE
                     VQQVQLSKDKKYFYLTTNEEHPGEQHFYRLSIADGRKERITNMTGAHQVIVSPDEKQL
                     AILYSYTNKPWELYLQENKPGGKLEQITNKAQSDEFKSYPWKVPEVITFTANDGAQVY
                     ARLYKPANPHPDKPAVLFVHGAGYLQNAHKWWSSYFREFMFNNMLADNGYYVMDIDYR
                     GSAGYGRDWRTGIYRHMGGKDLSDHVDAVNFLIQNYGVNSKNVGLYGGSYGGFISLMG
                     MFTEPDVFAAGAALRPVTDWANYNHGYTSNILNEPFTDSIAYRRSSPLYFAEGLKGHL
                     LICHGMVDVNVHYQDAVKLAQRLIELGKDNWELASYPMEDHGFIEPSSWTDEYKRIFK
                     LFESVLKK"
     gene            10083..10997
                     /gene="accD"
                     /locus_tag="PLO70_05230"
     CDS             10083..10997
                     /gene="accD"
                     /locus_tag="PLO70_05230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQZ73893.1"
                     /translation="MAKQFEDFDANLTGDEGKAEETRASWFKRIKKGINTKTSEKKET
                     PEGLWVKCPECNFICTVTELKENLFACPKCSYHHRINSQEYFEILFDNSEFTELFDNI
                     RSKDFLEFTDLKPYKKRLEDIWSKTDLKDSMRVAAGKVGGNDIVIACMDFEFIGGSLG
                     SVMGEKFSRAVDHCIEHKMAYMVISKSGGARMMESAFSLMQLAKTSGKLSQLSDARLP
                     YISLLTDPTFGGISASFGMLGDLNIAEPGALIGFAGPRVIKETIKKDLPEGFQRSEFI
                     LEHGFLDFIVPRKELKDKLATVLSLFKN"
     gene            11057..11494
                     /gene="smpB"
                     /locus_tag="PLO70_05235"
     CDS             11057..11494
                     /gene="smpB"
                     /locus_tag="PLO70_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQZ73894.1"
                     /translation="MAELRNRQAYHEYFIDAKYEAGMVLLGTEVKSLRAGRASFNDSY
                     CLIHKGEIWIKSLHIAEYSHGTVNNHDPLRDRKLLLEKREIKKIEAKLKEKGYTLVPL
                     RIFFNEKNLAKIEIGLARGKKLYDKRESIKQKDVEREIKRYLK"
     gene            11550..12488
                     /locus_tag="PLO70_05240"
     CDS             11550..12488
                     /locus_tag="PLO70_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73895.1"
                     /translation="MKKGLFILCLVVSLYSHSQSVFGYWYGYANVKTNTAANNYLVEM
                     VLQPEKNYVKGILNYYFKNTYRSIQVKGNYNAATRQLSLYDIPVVYHGSYSNFEVDCI
                     MNMQATLRVAQAESALTGSFIALPDYKYTCANISFKLTLNADISKKDSVLKAISEYKE
                     NYQVWEPAATDTSIAANIIPRKVVNYVIEKEFSERENEITNEIEVDSDSLRVDFYDNG
                     EIDGDIISVFYNQKLIMFNQKLTHRSIHIDLLLDSTRAINEVSMFAESLGLIPPNTAL
                     MRIHDGKKQYDIRISSTLQKNATIRIKRKKDGKKSE"
     gene            complement(12492..15008)
                     /locus_tag="PLO70_05245"
     CDS             complement(12492..15008)
                     /locus_tag="PLO70_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HQZ73896.1"
                     /translation="MSAAKNKITEVQNNDGGLSGQYKTWFLDYASYVILERAVPAVED
                     GLKPVQRRILHAMKEMDDGRYNKVANIIGQAMQYHPHGDASIGDALVNMGQKDLLIDT
                     QGNWGDVRTGDDAAAARYIEARLSKFALEVAFNAKTTEWQLSYDGRKNEPVTLPMKFP
                     LLLAQGADGIAVGLSTKILPHNFCELIDAAVKYLRGRKFELYPDFLTGGMIDVADYNQ
                     GKRGGKVKVRAHIEEVDKKTLAIRSVPYGVTTTQLMESIVKANDQGKIKIKKVTDNTA
                     ADVEIQVDLAPGISPDITIDALYKFTDCETSISPNACVIVEQKPQFLGVQELLKLSVD
                     KTKDLLEQELKIRLNELQEKWHYTSLEKIFFEEKIYKELEKKHETWDKVIESIDKAFI
                     PFKKQLKRDINREDIIKLTEKPVRRIYRLDIDELNNQIKGLETEIKQVKHDLANLIDF
                     AVAYFENLLKKYGKGRERKTEIRQFEIIEAKNVAIANTKLYVNREEGFIGTGLKKDEL
                     LFECSDLDDIIVFAKRGIMKVVKVSDKTFIGKDILHAAVFQKNDERTTYNMIYSDGKG
                     GLSYAKRFNVTGVTRDKEYDLTKGDEKSKIHYLSVNPNGEAEVVKIVLTPGCAARIKE
                     FDFYFEELAIKGRSSMGNQVTKYPIKSVRFKEAGKATLSGRKLWFDEKFGRLSAEEKG
                     TYLGSFEPDDRILVIYNDGNYEVTDQEMTQKIDPEKVLYIGKFDPEKVITAVYVDNDK
                     KQYMVKRFKIETSTLKTKFMFIKEGDGNYVEAVSTDEEPILAMQQGRGAQIRKAKLKI
                     VKIAEVTGWKTVGTKLADYSKSTEMEWVKSKESNQAELFE"
     gene            complement(15022..15873)
                     /locus_tag="PLO70_05250"
     CDS             complement(15022..15873)
                     /locus_tag="PLO70_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HQZ73897.1"
                     /translation="MIHIVFNESEVELMKQVIELDETMSGDVIQIRDDYAVGPLSALD
                     TEEGRQSRLNWWKDLLSYSHYQESQVHHFDDRETVQLLKSKLDENPDEELWIWMGQNQ
                     HDVCGYYWLMPQLKDYQGRVMVLFLNNLPFINEKGHIFYPSWLNEIQPKEFIKAKKLA
                     RPITLSEFEVDPDEWVRIVNDGGFVRILEGGKKIASKDDTFYDKEVLKNLTPEWQKAW
                     RLVSNTLHRMPVKTGDVFIEWRIRQLIEQGKIEVTGDVSKGWKDFDVKLPGTKQQETE
                     LTEEALQ"
     gene            complement(15870..16664)
                     /locus_tag="PLO70_05255"
     CDS             complement(15870..16664)
                     /locus_tag="PLO70_05255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73898.1"
                     /translation="MFEFHADRKRYFDIQVLNAEKYVIPFIEEKFPVKAGMRILEIGC
                     GEGGVLKAFINKGCEGVGVELDAPRIVDANKFLTEDIAAGRLRFVVKDIYEVDVKKDF
                     NGLFDIIVLKDVIEHIHDQAKLIGWMKNFLKPGGIVFFGFPPWYMPFGGHQQICKSRI
                     SRLPYIHLLPKGMYKWVLRTRKENVEEMMEIRDTGISIERFEKICKKEGYTLFHTGHY
                     LINPIYEWKFGWKPRKQIGLIKLIPFFRNFLTTCVYYIIQPDNKLK"
     gene            complement(16676..18574)
                     /locus_tag="PLO70_05260"
     CDS             complement(16676..18574)
                     /locus_tag="PLO70_05260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQZ73899.1"
                     /translation="MAKDKETTNLFEYTEESIKSLDWKEHIRLRPGMYIGKLGDGSSP
                     DDGIYVLVKEVMDNCIDEYTMGHGKTVELTIDGKSVTIRDYGRGIPLGKVVDVVSKIN
                     TGAKYDSKAFQKSVGLNGVGTKAVNALSSFFKVTAIREGKEKTAEFERGFLIKEHKES
                     KTGEQNGTMVTFIPDETVFKNFKFHPEFLENQLWNYCFLNAGLKIIFNGKSFVSKNGL
                     LDLLQRKTNEDEIRYPIIHLTGNDIEVAISHTNEYGEDIYSFVNGQHTTQGGTHQQAF
                     REAYVKTIRDFFKKDYDASDIRTGIVAAVSVRVVEPVFESQTKTKLGSVNVDIDGPSM
                     RQFVGDFLSKELDNYLHKNPSIADGLKKRIEQSERERKELAGIKKLANERAKKANLHN
                     KKLRDCRHHLNDDAPAKGKEEFVAKQKETTIFITEGDSASGSITKARNVETQAVFSLR
                     GKPLNCFGLTKKVVYENEEFNLLQHALNIEEGLEGLRFNNVVIATDADVDGMHIRLLL
                     MTFFLQFFPDLVKHEKVYVLETPLFRVRNKQETIYCFSEQEKLDAMKKLGTKPEVTRF
                     KGLGEISPDEFGQFIGAGMRKQLMRVEEGEHIQQLLEYYMGKNTPDRQEFIINNLKVE
                     LDIVEEIV"
     gene            19166..19435
                     /locus_tag="PLO70_05265"
     CDS             19166..19435
                     /locus_tag="PLO70_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73900.1"
                     /translation="MYTYDLLETGCYYLVKEKEESPISLIKIAVVTDHCVFVQRFNEP
                     METTWKLKKDNLHDIIECLSDETVKSWEEYYRSNEDAFYEGDDEE"
     gene            19657..21129
                     /locus_tag="PLO70_05270"
     CDS             19657..21129
                     /locus_tag="PLO70_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQZ73901.1"
                     /translation="MSIFRKKGNACLFVFLFISQVIAAQPSPPDEFLPKDYLPASFHA
                     GRRDALRNSMPDSSVVVIFAYPTRTFSNDVEYIYHANPDMYYFSGYKEPHSLLMVFKE
                     MQTDSAGYNYNELLFVQKKNALAEQWTGKRLGVEGAKEKLGIENVFNGVDFKKFNIDF
                     SKFKKIICDKPSVDIPNGFDNADLYDLVEQFKQKSGMAEKKQMGRFDNRLYNELTGKL
                     RRIKTPEELSLLRKAVEISCLGQNEVMKAVHPNMSELEIQGLHEFVHKKLGAEAVGYG
                     SIIGSGENGCVLHYMENIKTKIGTSMILMDVGAEYHGYTADVTRTVPVDGKFSAEERA
                     IYQLVYDAQEAAFSTLKDGSKWSDASNQAREIIANGLMQLGIIKEKSEVGKYFPHGLG
                     HHIGLDVHDRGSMETLRKDMVITIEPGIYIQSNSDCDKKWWGIAVRIEDDLLITENGY
                     ELLSRYAPRKIEEIEKMIAQKSALDDFKLPPLESGKKKGF"
     gene            complement(21181..22869)
                     /locus_tag="PLO70_05275"
     CDS             complement(21181..22869)
                     /locus_tag="PLO70_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73902.1"
                     /translation="MKRQIVYLLALGLLVIIGCQKELSFEGANTPAQGSLQSDASGDC
                     LPKTVNGTYEAGTALVPATNTISVDINVTQTGTYLVTTDTVNGFYFSASGTFTALGNN
                     TVTLRSNGTPFAAGVNNFVVSFDSTFCDIQVTVLPAGAGGPAVFTLEGAPNACTGAIV
                     NGSYATGLALTASNTVVINVNVTTIGTYNISTTYQGMVFSGTGAFINTGVQAVTLTGS
                     GTPTTGGANTVPVTIGTSTCSFVVNVSSPAVGTLGGAPTACTPATVNGIYRVGDALTG
                     ANTVQVQINVTTGGVYSISTNTVTGFSFAGTGTVTAGPNQLIILTGTGTPATAGPQTF
                     TVTFGTSSCTFTVDCLPALSNDYFPRTTNSNWSYEIDDDPLDSLYRTATSGTISAVSN
                     TFNIFMQEDGVAPGLDSSGYYNRNIATGEYNEWFDAGSYLGYDNPLWASYIFLKDNVP
                     VGTSWNSQAFDGIATLPPPTPFSIRFKYSILQKDVPVSFTTSTGTMNFTNVIVVEERF
                     EMFSTATSSWIDMTSQIGSGKAYYARGIGLIKYEVLNTTGAVAGQQELRRYQVF"
     gene            23000..23692
                     /locus_tag="PLO70_05280"
     CDS             23000..23692
                     /locus_tag="PLO70_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQZ73903.1"
                     /translation="MRNVLLCLLLLPVLSCSSQTGDETRVGADAFEKLIARKDVQVLD
                     VRTANEFKGGHIKNALQADWTDRHEFAERLKYIDKDKPVYVYCLVGSRSTSAADWMRQ
                     TGFKNVIELTGGINAWKRAGKPVDGSGSERQMTMTEYMAAIPADKTMLVDFGAAWCPP
                     CVKMEPVLNAIKADKSLHFSFLKIDAGVHTELMKSMNIEPIPVFIIYKKGKEVWRKQG
                     IVSKDELAAQLK"
     gene            23710..24645
                     /locus_tag="PLO70_05285"
     CDS             23710..24645
                     /locus_tag="PLO70_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ73904.1"
                     /translation="MKRKKFLQRSAAVIGSSLLPSFAFAKETGKKSLRFAHLTDMHLK
                     PGLIPETGTAKALHHVQQLKPKVDFIINGGDAIMDSLGADKQNTEIQWNLFKGTLQRE
                     NSLPVYHCIGNHDIWGWFIKQDRPENDRLYGKVWVVETLEMKNRYYSFTKGNWHFIVL
                     DSTQLNPAGGYIAKLDTGQLDWLQQELASAPAGKFICLVSHIPILSICAGLFFDKTES
                     NGDLKIQRNLMHTDFIQLKKIFLKFPNIRVCISGHIHLQDKIDYLGIKYYCNGAVSGN
                     WWKGAFQEFEPAYAIVELFDNGTSKRTMIKYEQTN"
     gene            24629..25666
                     /locus_tag="PLO70_05290"
     CDS             24629..25666
                     /locus_tag="PLO70_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQZ73905.1"
                     /translation="MSRQINRILIMLSAALLLSEMIIAQSPHVEVLTFGTKTSIRGLS
                     VVNDNVVWVSGSNGTFGRSTDGGKTWKWSVVKGFEKNDFRDIEAFDANTAIVMSVDAP
                     AYILKTNNGGESWKIVYENKTKGMFLDAMEFWNEQAGIVIGDPVEGRFFIARTFDGGI
                     SWQDIPYEKRPVADSGEACFAASGTNVRVLDRDEAVFVSGGLKSRLFSKKTPVILPVI
                     QGKETTGANSVAVWDTHKLRGGKRMIIAGGDFNADTSVTKNCFYTTNKGKTWNVPEIA
                     PHGYRSCVEYFSKKKVYSCGLNGVDYSKDGGKNWEWISKEGFHVVRAAKEGKTVFLAG
                     GNGKIAKIVWK"
     gene            25691..26365
                     /locus_tag="PLO70_05295"
     CDS             25691..26365
                     /locus_tag="PLO70_05295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ73906.1"
                     /translation="MLKKFIEQMRLKAIARQLRKPNGAMGHKVARKMNEANEFLYDFT
                     LDEMLIADNHSILEIGFGNGKFFDKLLKKADNLKITGIDFSDSMVKSAKENNPAAIAD
                     GRLTITKGKSDNLPFANNSFDRIFCINVAYFWDEPDKHLQEIHRVLKPKGKFCTTVRT
                     KESMQYMPFTRYGFRGYTEDDWKLLSHKNNLTYINGVLVNEPPIEHEGSTVRTQSLCF
                     ITEKKR"
     gene            complement(26370..26954)
                     /locus_tag="PLO70_05300"
     CDS             complement(26370..26954)
                     /locus_tag="PLO70_05300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HQZ73907.1"
                     /translation="MKNTVLFICFLLSAYTVIAQEKPEGLFISSKAPDFKAKDQFGNE
                     IRLKDLLKKGKVVLVFYRGQWCPYCNKELSRLQDSLQFIRDKGAEVVAVSPEKPENVA
                     VTVEKTKAEYPILYDEGLKIMKAYDVEFEMEENTVTRYRNAGLDIEKNNGANGRFLPV
                     PAVYIIDKESTVTYRFFEPDYKKRPSVKELLKNL"
     gene            27071..27337
                     /locus_tag="PLO70_05305"
     CDS             27071..27337
                     /locus_tag="PLO70_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73908.1"
                     /translation="MPNSHSEKYVSNLLIGFSALIGFALITFYAIFELKKDSSWYFWA
                     IVAAFLLCTGIYFCLIAIVHKVKSDFNRRAKQREQQKTFTADRI"
     gene            complement(27360..27698)
                     /locus_tag="PLO70_05310"
     CDS             complement(27360..27698)
                     /locus_tag="PLO70_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQZ73909.1"
                     /translation="MRTIILLFASNVFMTFAWYYHLKHQGWPLWKAILISWLIALFEY
                     CLMVPANRFGKVDGFNLFQLKMIQEVITLLVFAAFAVWIMKEPFHWRYLVSFAFLMGA
                     VYFMFSNRFG"
     gene            complement(27905..31441)
                     /gene="smc"
                     /locus_tag="PLO70_05315"
     CDS             complement(27905..31441)
                     /gene="smc"
                     /locus_tag="PLO70_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQZ73910.1"
                     /translation="MRLKSLEIKGFKSFADKTIVSFDDNITGIVGPNGCGKSNIVDSI
                     RWVIGEQKISHLRSENLDSLVFNGSKTRSASGLAEVSLTFENTRNLLPTEFSTVTVTR
                     KFYKSGESEYRLNDVQCRLKDIQNLFMDTGISTDSYAIIELGMVDDLIKDKENSRRKM
                     LEQAAGISIYKTRKKEAKLKLDATEQDLARIEDLLFEINNQLKALESQAKKAEKYHEI
                     KKDYREVSIELAKASLEGFNLTYKELNEQQDAETDKKHKIEAEIGTQEAELEQEKLSF
                     IEKERQLQSMQHAFNDMLQTVRSKENDKNLSAQRLEHLQEKEGSLKEFLQKAEGQLKG
                     IEESIGFTNQQVQDEEKALEILGEQLTQLNNEVNSKREILDEKRRMLDNLRSEYQQIQ
                     RSQFDAEKKVAVADTSVQNLLRVIHQIEEERVHREEQRHQLDNERILKEKELGIKKVD
                     LEQLQQHQENTKEQILQTQAVLDSLRNNLADETRKLDAKKNEHDLLKSMIDSMEGYPD
                     SVKFLHNNSNWSHNSPILSDVLYVNEEYRTALENVLEPYLSYYVVNNLQEGLQAVHLL
                     DENKKGKANFFLLDKVNQGEAVVSSHQLEGAIPALSVVEVEEKYKKLAEHLLGNVFIA
                     DSEDALQNSNGFVVIEKHGKYVKGKYSLTGGSVGLIEGKKIGRAKNLEKLQDEIAAQD
                     MVVEALRSEIQARHNEVIAFNEDLRETAVRETEREIQQLTNEVFSLHNKLENLHSMQT
                     NSQHRLEELNLQMQQTHSSVEGVRNQLAEFNELLLANSAKLAEADEAFRLTESDYNEA
                     TRQYNEFNLNVTKQQSKITGLKQELDFKNNQLSDLRQQIENNTAQLADTGNNIGQSAQ
                     SLKEIEESLLELLRRKEDEERNLNEADQAYYNFRNQLNEKESSIRHQVKEKEQIEYLL
                     AAIKDKLNELKLQLAGMKERLNVEFRIVLEDILGEARSGEVPVEELQEKADRMKKRLE
                     NLGEVNPTAIEAFTEMKKRYDFIVEQKTDLVNAKDSLLKTIEEVEATANQKFLDTFHQ
                     VRNNFQKVFKTLFTDDDQCDLVLDNPENLAESGIDVIAKPKGKRPSSLTQLSGGERTL
                     TATALLFAIYLIKPAPFCILDEVDAPLDDANVGKFTNMIRQFSENSQFIIVTHNKMTM
                     STVDVIYGVTMQEPGVSKLVSVDFRNLEQNQN"
     gene            complement(31884..32852)
                     /locus_tag="PLO70_05320"
     CDS             complement(31884..32852)
                     /locus_tag="PLO70_05320"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQZ73911.1"
                     /translation="MKANLFVVASLFLILAAGCKKIKLNKVPIADAGPAQTIQLQTDS
                     VILSGNGTDEDGTIMGYLWSKVAGPNIPAIHTPGAATTRVTGMIAGVYRFQLMVVDDD
                     GATGLDTVSVIILPAQITTVTIQPGSDGQDALVITKDGDNTSPNLNIGQHTELVYSKW
                     TYGGQGFGEGVMRSYIKFSAVSSIPASAEILSAKLSLYGVASSGFTPEGNSHFPGSPY
                     GSGPDNPGWIKRVTSNWDEATITWNNKPGTADAGQASIPGTTTQWNFNATDIDVTEIV
                     RTMVSTNSNYGFCLTLQNEAIYRNVIFSSSEAIDAARRPKLVVVYR"
BASE COUNT         9899 a         6678 c         6456 g         9914 t
ORIGIN      
        1 aaagagggaa aacctttatc tcttgaactg ttaagagatt actataaaga attcagcaac
       61 ttgtcacttt ctcaacgtat tagtctgcat aaactgagag aagaccgggc tgatgtaatt
      121 gttcctgcat tacttattta tatcaatgta atgcgctggg ctgatgcaga agaaatattt
      181 gtacccaaaa tcgggttagc agatggcctg atccacaact tgtatgaaga agtacgatta
      241 aaagaaatag aagtttgatt ttcttttctt aattacaaat catttttatg tctgtcaata
      301 aggaagtaaa aagagtcacc acaaacacgt tgctgaaaat gaaagcagca ggtgaaaaga
      361 tatctatgct taccgcctat gatttttctt ttgcaaaaat tattgatgga gcaggcattg
      421 atgtaatact ggttggtgat tcagcttcca atgtaatggc tggacacgaa acaacattgc
      481 cgattacact ggatcagatg atttatcatg cacaatctgt cattcgcgga gttgatcgtt
      541 gtcttgttgt agtcgatctg ccttttggat cttatcaatc caattcagac atagcgctgg
      601 cttctgctat ccggatcatg aaagaaactg gagcccattc tgtgaaaatg gaaggcggac
      661 aagaagtaat tgattccatc aaaaaaatag tttcagcagg cattcctgta atgggtcacc
      721 ttgggctgat accgcaatca atttataaat ttggcactta tgctgtacgg gcaaaagaag
      781 aagtggaagc tgagaaacta agaaaagatg caagtttatt gcaggaggtt ggttgttttg
      841 ctattgtatt ggaaaaaatt ccggcagatc tggcaaaaga agtttctgca agtttaatga
      901 ttccaaccat tggtataggt gccggaggtg attgtgacgg acaggtattg gtaatgcatg
      961 atatgctggg catcaatacc gaattcaaac caaggtttct tcgtcagtac cttaacattc
     1021 atgaacaagc cactaaggca gtgcaacatt atatcagcga tgtaaaatca ggtgacttcc
     1081 cgaatgataa cgaacagtac tgatccactc acctgaagca acgaatttta tatttctacc
     1141 tttacagttc aaaaaaaaag tatggaattt cttaagacac ttaaaatcga agaaaagaat
     1201 aaaggcgtga gtaccggcac caattggatc aactcaactg gagaaaccat agactccttc
     1261 tctccagttg atggtaaaaa aattgcttcg gtaaccgcaa cggataaaga aagctatgaa
     1321 gcagttgtta agaaagcgga agaagcattt aaaacatggc ggttagttcc tgctcccaaa
     1381 agaggtgaaa ttgtaagaca aatcggtgag gctttacgcc aacataaaga accattgggc
     1441 aagcttgttt cttatgaaat gggtaaaagc ctgcaggaag gatacggaga agtgcaggaa
     1501 atgattgata tatgtgattt tgcagtcggt ttatcaagac aattatatgg attgacaatg
     1561 catagcgaac ggccgggtca cagaatgtat gaacaatatc atccgctggg aatagttggg
     1621 ataatctcat cgttcaattt cccggtagct gtgtggagct ggaatacaat gctggcatgg
     1681 gtttgtggtg acgtatgcat atggaagcca tcagaaaaag ttccgctgtg cagtattgcc
     1741 tgccagaaca ttatttcaga agttttcaaa aagaacaatg tcccggaagg ggtttccggt
     1801 ttggtaattg gtggccgtga agttggtgaa tggatggcgg cggatataag aatcccgttg
     1861 gtgtcagcca ctggttctac ggcaatggga aaggctgtgg gtgcaacagt tggagcaaga
     1921 ttaggaagag ctttgttgga attaggagga aataatgcca ttatcatttc gaaggaggct
     1981 gatttggata tagcaatact tggatctctt tttggagctg taggtacagc tggccaaaga
     2041 tgtaccacca caagaagatt gattattcat gaagaaatat atgatacagt aaaaaacaaa
     2101 cttatttcag catacaagca gctaagaatt ggtaatccac tagaccaaaa aaatcatgtg
     2161 ggtccgctga ttgacaaggc agcagttagt atgtatttgg attcacttga aaagtgcaaa
     2221 atcgaagggg ggaattttat cgtagaggga ggcgttatgt cgggtgaagg atacgaaagt
     2281 ggttgctatg tgaaaccttg tattgctgaa gctcagcctg gtttcaaaat tgtgcaacat
     2341 gaaacctttg cccctatact ttatctattg aaatataaca caatagaaga agcaattgct
     2401 attcaaaatg aagtaccgca aggattatct tcttctataa tgacactcaa tttaagggaa
     2461 gcagagcagt tcctgtcaca tgccggtagc gattgcggca ttgccaatgt gaacattggc
     2521 accagtggtg cggaaatcgg gggtgctttt gggggtgaaa aagaaaccgg cggtggtcgt
     2581 gaaagtggaa gtgacgcctg gaaagcttat atgaggaggc agacaaatac tatcaatttc
     2641 agtaattctc tgccacttgc ccagggaatc aggtttgatg tataaaatag attggcgttt
     2701 tttacgttat gtaattgaaa tggctaaaag cttttatatt attatataca ggctttaatg
     2761 caattttgca acaactagcc gccaaaatat caactcctat gagaaagtgt attctttcat
     2821 tttcctttat tatcagcctg ttggttacca ctcacgcaca agttagagtg gcaattgtag
     2881 ctggcggaca ccagtcgtca gttttagaag aaaatgatct gcccaactgg agtgaattaa
     2941 aaagcaatta cacaggtcgg attggatttc ataccggttt tatcgcagac attgcttttt
     3001 ctccaaaatc aaaattattt tttcaaccag gtgttgttta ttacaataag ggacgcaaat
     3061 tttcgcaacg tttcgacccc ccacttggta ttataatcaa ccaaaaaagt actcaattcc
     3121 tcaattatat tgatgtaccg ttaaacctgg tgctgaaatt tggcaagaaa accaaattca
     3181 ttattggtgg aggcccctat ggttcgttct tttacagcgg taaagaaacc gcccaaacca
     3241 ttactacagg aggtataact gaatcaactg agaataatga tttatcagtg ggcaatcagc
     3301 ctggtcaatt caggacaact gattatggca ttaacggtct agctggtctt gaattcaggg
     3361 ggttcttcat tactgccaat tatagccgcg gattaaatga tttttatgaa tcaactgttt
     3421 ataatggaac gttccgtcat gaagtcattg gagggacttt gggtgtattc cttggcaaaa
     3481 cagaaaaaat tgagaaaaaa attaaagaca tagacaaaga tggcatcaat gatgataaag
     3541 atcattgccc tgaagaaccc ggaactgcag ctacaaatgg ctgcccggat aaagacgctg
     3601 atggtatagc cgataaagat gataagtgcc ctgatattgc cggtttgatt aaatataatg
     3661 gctgcccggt tcctgacaca gataaagatg gcattaatga tgaagaagat aaatgtccaa
     3721 acgctagtgg ctttgcccgc tatggcggct gcccggttcc tgatacagat ggcgatggtg
     3781 taaatgatga agaagataaa tgcccgactg ttaaaggcac aaaacaaaga aatggctgtc
     3841 ctattgaaga aataaagaaa gaaatagttg agaaagtgaa ttttgctgcc aaacgaatcc
     3901 agtttcagtc ggccaaagct gtgttgctcc ctcagtcatt gaaagtactc gatgaagtag
     3961 ctaatatatt aaaagataat ccggaactga gcctgtccat tgaaggacat accagtggtg
     4021 atggaatttt tgaggccaat atgaagctgt ctgatgaaag agccaaaaac gtaaagggct
     4081 accttatttc aaaagggatt gatgctgcca ggcttacagc caagggcttt gggcctactc
     4141 aacctataaa taagggcaaa acaactgctg agaaggcaca aaaccggagg gtagaactta
     4201 aattaagcaa ctgatttcgt ttatcatacg tcaaaaaaaa gctaaagcct cgtgaagcgg
     4261 ggcttttatt tatgcaaaat gtatgaatgg taatcagtat ttttacgact ttatcaaatt
     4321 tgcagtataa aatcaaacaa atgaatcgaa taatgaaacg taccacttta ctagctatcg
     4381 ggggcatgct gacttctgcc cttttcgcac aaaaagatga agtacccaaa ggctggcata
     4441 tgctggataa agccagttcc ggttataatg gcatcagtgt cgataaagcc tacgaatttg
     4501 taaaaacaaa aaaactgaaa agtaataccg tcattgttgc ggttattgat tcaggaattg
     4561 acactctcca cgaggatctt aagcctgtgc tatggaaaaa tactaaagaa atacccggta
     4621 atggtattga tgatgataag aatggttatg tggatgatat ccatggctgg aatttcctcg
     4681 gtggccgtga tggacgcagt gttgataaag attcatacga agcagcaagg gtctatcaca
     4741 aattcaaaac aaaatatgaa ggtaaagatg taaatgtttc ttctctttca aaggaggatg
     4801 ctttcgaata ccaaatgtgg aagcgttctg aaaaagaagt ggcaggtgat agcaaaagca
     4861 gtggcatgga gctcattttt atgaaaagag cttacacaaa ttgtcttaaa agcgacagta
     4921 ttctccgcac agcaatgggc aaagaaaaat atacaggtaa agaactggga gaatttacat
     4981 cagatgatcc aaacgtaaaa aaggcaaaga atacattatt tagcctgatg tcgggtaatg
     5041 atgcacttga aactacgaat gtggaatttc tggaaggatt cggtgattat gtttccgggg
     5101 aagaaaagaa agccgaggcg ggaagtactc ccccaactcc ttaccgggca gatattgtaa
     5161 aagataacta cgacgatttc aatgacaaat tttatggcaa taatgatgtt atggtaagca
     5221 ataaatctgc tctgcatggt acccatgtat ccggaatcat cgctgctgcc cgtaaaaatg
     5281 gtaaaggcat ggatggtata gcagataacg taaagataat gtcattacgg gccgtacctg
     5341 atggagatga acatgataaa gacattgcat tggccattcg ttatgcagtt gataatgggg
     5401 caagggttat taatatgagt tttggtaaag gattttctcc tgagaaaatg tgggtggatg
     5461 aggctgtcaa atacgctgaa agtaaaggtg tgctcatggt acatgccgca ggtaacgatg
     5521 caaagaatct tgacactacg ttcaactttc caacaccaca gttactggat ggtagccgtc
     5581 cggacaactg gatcacagtt ggggccagtg gtgatcctaa agccggtggt cttactgcca
     5641 atttctctaa ctatggtaag aaagaggtag atgtatttgc ccccggtgtt aaaatttatg
     5701 caactgtacc tggtggcaac acttaccaaa acctgcaggg taccagtatg gcatcaccgg
     5761 ttgtggctgg tctcgcagca tttattatgg agtattatcc taatcttagt gccaggcagg
     5821 taaaaatggt aattgaaaaa tcagcgcaaa agcctgccga taacgtaaaa aatccaggta
     5881 atgatgaaat ggtaaatctc ggggaattaa gcagaaccgg tggtattatc aatgcctatg
     5941 aagctattaa attagcggct acaattaaag gtgaaaggaa accttcacct gttaaaccct
     6001 cgaccaaagt aaaagctaaa gtaaagggat aaaattttat ttttttagaa agcccggcca
     6061 atgccgggct ttttgtttgt cagtataatg ctggttattc atcggatcag aattctcaaa
     6121 taccacatat ttttgaataa atacaggctg tatgatttcc tataatacca aagactggtt
     6181 tactttcatt acccgttttc ataaggcaga tacattccgc caactttttc ctcttatcct
     6241 tgccatcagt gcctattcgg gcattattgc ctggctggaa ctggattact ggaaactctc
     6301 agaaaaagat tatgtccgta atatccctgt catgcatact ttattgggct ttgctatttc
     6361 gatgttactg gtattccgga cgaacacagc ttatgaccgc tggtgggaag gtcgaaagct
     6421 gtggggcagc cttgtaaata atagccgaaa cctggccatg aaactgaata caattttgcc
     6481 ggcagaagac aatagtcagc gttccttttt cagaaaagtc attcctgcat ttgcttatgc
     6541 cttacataat catttacata aagaacaaac caggattgaa ttgtttgaag aggaggaaca
     6601 taaacatgta tttcagcgta tagataaggg aaagcatatt cctaaccaga tcgcactact
     6661 tatgtaccag catatacatc aacttttaaa ggaggagaaa atcagtggcg aacagttatt
     6721 attcctgaat tccgagctac agtcttttac agatatttgt ggcgcctgtg aacgcatcaa
     6781 gaatactccc atcccctttt cctatagtgt ttttataaag aaatttattt tcttttatat
     6841 tatgacactt ccgtttgggt atgtgttcca gttgggattt tatgtggtgc cggttgtggc
     6901 atttatcttt tatgtactgg ccagtcttga actgatagcg gaggagattg aagatccttt
     6961 tgggggtgat tctaatgatg ttccaacggt tgcactggca caaaatatac atcgtcatgt
     7021 gacagaaatt atctgatcgt cagaaattga tagctacaaa ggcaactttt actatttttt
     7081 tcccgttaac ctataaccaa aatgaatact atgtcaaaat tttcctcatt tatcctattg
     7141 actattctga caattacatt actgattgcc tgtagtaaag acaacgatac tggcgcttca
     7201 cttgattgca gtaccgtaac caacaaagcg tttgccgcag atataaatcc aattattcaa
     7261 tcttcctgcg cctttagcag cggctgtcat ggcagtggca gcactaatgg ccccggcgtc
     7321 ctgacaagct acacttcggt ttttaatgcc cgtactgcca tccgttcagc agttgcttca
     7381 ggcagtatgc ctcagggaag cagcctggcc acgaaccaga aaaactccat tctttgctgg
     7441 attgatagtg gtgcacccaa taattaaata aaccctaatc cgcttataca tttttacaag
     7501 gattggtatt tagctatctg ctttctcaat atatttatag ctaaatatcc aaatgatgcg
     7561 gctacttact gtaattcttt cttttatatt tttttcctgc actgtttatg ctcagtcaaa
     7621 acttgacaaa ttaacggtgg ataaaatcat gcgggaccct aagtggatgg gaacctctcc
     7681 atcatctcca ttttggagta atgacgggca acttctatat tttatgtgga atccggagca
     7741 agcccctgct gattctttgc attatatttc cagtttaagt aaggttcctg taaaagcttc
     7801 tgttgaacaa aaacaagctt tgagtgcgca agggaattat atatataacc agtcaggaac
     7861 ggcttatgtg tatgcccgta atggagatat ttattttaca gaggttaaaa caggtaaatc
     7921 taaacgtatc acacagacaa cagataacga aagtggtccc cggttctgtt ttaatgaaac
     7981 aaagatcgtc tatagccgta atcaaaacct ttatgcatgg gatattgcag gtggtgagac
     8041 catacaattg acgaatatta aaactggtga aagttcttct tctgccgctg gtggctttca
     8101 acgaacaggt gctcaaccaa caacaagatc aggtggcaac ccacaggaac aatggttaca
     8161 aaatgaccag ctcacctatt ttcaggtatt aaaagacaga aagagtaaaa gagagatggc
     8221 tgatgcttac aataaatcat taccaaaaga aaaagaactg cgcagtataa atttggaaga
     8281 taaaactata cagggacttt ctatcagccc tgatgggagg tttgtaagtt accggctttt
     8341 caaagcagcc accggatcaa aaacaaccat tgtccccagc tatgtaactg aaacaggttt
     8401 cactactgat attccttcca gaacaaaagt tggttcatca caaggcagtt cagaattttt
     8461 tatttacgac agggaaaaag atactatcct caatataaaa acagattcaa ttcccggtat
     8521 aaaagagctt ccggattatg taaaagatta tcctaaacaa ttagaagaaa gacaaaaaaa
     8581 ggcagccaat cgcctggtga attttacagg attgacctgg tcgcctaaag gaatgaatgc
     8641 agtcattgaa atccgttcac aggataataa ggatcgttgg ctgatgcaat gggacaaaaa
     8701 tacaggggga ttaaaattgt tagaccgtca gcgggatgaa gcatggattg gcggtcccgg
     8761 aattggtggt tttggcaata ccaataccgg ctggatcgat gaaaatattt tctggtaccg
     8821 gtcagaatct tcaggttatt ctcatttata tactgtcaat attacaaccg gagtaaagaa
     8881 agcgctgaca tcaggtagtt atgaagtgca acaggtacag ttgtcgaaag ataaaaaata
     8941 tttctatctt accactaatg aagagcaccc gggtgagcag catttttaca gactttctat
     9001 tgctgatggc agaaaagaaa gaataaccaa catgaccggt gcccaccagg taattgtatc
     9061 acctgacgaa aagcaacttg ctatattata ctcctacacc aacaaaccct gggaattgta
     9121 tttgcaggaa aataaacccg gaggaaaatt ggaacagata accaataaag cacaaagcga
     9181 cgaattcaaa tcttatccgt ggaaagttcc cgaagtaata acttttactg ccaatgatgg
     9241 cgcacaggta tatgcaagat tatataagcc tgccaatcca catcccgata agccggcagt
     9301 tttgtttgtt cacggtgctg gttatttaca aaatgcccat aaatggtgga gcagttattt
     9361 ccgtgaattt atgttcaata atatgctggc tgataacggc tattatgtca tggatattga
     9421 ttatcgtggc agtgcaggtt atggccggga ttggcgaaca ggtatttacc ggcatatggg
     9481 tggtaaggat ttaagtgacc atgtggatgc tgttaatttt cttatccaaa attatggtgt
     9541 aaactctaag aatgttggtt tgtatggagg gtcatacgga gggttcattt cattgatggg
     9601 aatgtttact gagccggatg tcttcgcagc aggtgctgct ttacgacctg ttacagattg
     9661 ggctaactat aatcatgggt atacttccaa tatactgaat gaaccattca ccgacagtat
     9721 tgcataccga agaagttctc ccctttattt tgcagaaggg ttaaaagggc acttattgat
     9781 ctgccatggc atggtagatg tgaatgtaca ttaccaggat gctgtaaaac ttgcacagcg
     9841 gttaatagaa ttgggaaaag ataattggga gcttgcttcg tacccgatgg aagatcatgg
     9901 atttatcgaa cccagcagct ggactgacga atataaacgg atttttaagt tatttgaatc
     9961 agtactcaaa aaataaatta attatagagg ttcactaata tagagccgga ataaggaaat
    10021 tcatttcccg ttgattattc actattcaca acttccctat atttgtgtat caacaaaagg
    10081 ttatggcgaa acaatttgaa gattttgatg ccaatctgac tggagatgaa ggaaaggcag
    10141 aagaaaccag agccagctgg ttcaaacgca tcaaaaaagg aattaatacc aagacttctg
    10201 agaaaaagga aactcctgaa ggactttggg taaaatgtcc ggaatgtaat tttatctgta
    10261 ccgttaccga gttaaaggaa aatttatttg cttgtccaaa atgcagttat catcatcgga
    10321 ttaatagcca ggaatatttc gaaattcttt tcgacaatag cgaattcact gaattatttg
    10381 ataatatccg cagtaaagac ttcctggagt ttactgacct aaaaccatac aaaaaaagac
    10441 tggaggatat ctggagtaaa acagacctga aagattctat gcgggtagct gctggtaaag
    10501 ttggtggcaa tgatattgtg attgcctgta tggactttga atttataggc ggctctttgg
    10561 gaagtgtgat gggtgaaaaa ttttcacggg ctgtagatca ttgtatagaa cataaaatgg
    10621 cctatatggt cattagtaaa agcgggggtg cccgtatgat ggaaagtgca ttttcattga
    10681 tgcaattggc taaaacaagc ggaaaacttt cgcaactaag cgatgccagg ctcccgtaca
    10741 tatcattatt aacagacccc acatttggtg gtatctctgc atccttcggt atgcttggcg
    10801 atttaaatat agctgaacca ggtgctttaa taggttttgc cggacccagg gtaataaaag
    10861 aaacaatcaa aaaagatctg cccgagggtt ttcaacgcag tgaattcatt cttgaacatg
    10921 gttttctgga ctttatcgtg ccaagaaagg aactgaagga taaactggct acggtattga
    10981 gtttatttaa aaattaataa taagaaaata taatcaggaa gtaagaggta attttgcctc
    11041 ttattttatt ttatagatgg cagaattaag aaacaggcag gcttaccacg aatacttcat
    11101 cgacgcaaaa tatgaagcgg gtatggtatt gctgggaaca gaggtaaaat ccctgagggc
    11161 aggaagggcc agttttaatg acagttattg cctgattcat aaaggtgaaa tctggataaa
    11221 atcattgcat atcgccgaat actctcatgg tacagtaaat aaccacgacc cgctgcgtga
    11281 cagaaaatta ctcctggaga aaagagaaat caaaaaaatt gaagcaaaat tgaaagagaa
    11341 gggctatacg ctggtgccct tgcgcatctt ttttaatgaa aagaacctgg cgaaaattga
    11401 aattggcctt gccagaggca aaaaactcta cgacaagcgg gaaagcatca aacaaaagga
    11461 tgtagaaagg gaaataaaaa ggtacctgaa ataatactgt gtaaaacttc gtttcaggaa
    11521 ggcacgggat ttgccgatat tacaaattca tgaagaaagg attatttata ctctgccttg
    11581 tggtctcatt gtattctcac tcccaatccg tattcggtta ttggtacggg tatgccaatg
    11641 taaagacaaa cactgctgct aataattacc tggtagagat ggttctgcaa ccggaaaaga
    11701 actatgtgaa aggaatcctc aattattatt ttaagaatac ttaccgcagt atacaggtaa
    11761 agggcaatta taatgcggcg acccgtcaat tgagtttata tgatatacct gttgtttatc
    11821 atggttctta ttccaatttt gaagtagact gtataatgaa tatgcaggct acgctccgtg
    11881 tagcacaggc tgaatctgct ttgacaggtt cctttattgc acttccggat tacaagtata
    11941 cttgtgccaa tattagtttt aagcttacac tcaatgcaga tataagcaaa aaggattcag
    12001 tactgaaggc gatcagtgaa tacaaagaga actaccaggt atgggaaccg gctgcaacag
    12061 acacttctat tgctgccaat atcattcccc gaaaagtggt caactatgtt atagaaaaag
    12121 aattttcaga aagggaaaat gaaattacta atgaaattga agttgattca gattcattga
    12181 gagtggattt ctatgacaat ggagaaatag acggcgacat tatttctgtg ttttacaatc
    12241 aaaaacttat catgttcaat cagaaattga cgcatagatc tattcatatt gaccttttac
    12301 ttgatagtac ccgtgcaatc aatgaagtgt ctatgttcgc tgaaagtctt gggctgatcc
    12361 cgcctaatac agccttaatg cggatacatg acggcaagaa gcagtatgac atacgtatat
    12421 ccagtaccct gcagaaaaat gccaccatca ggatcaaaag aaaaaaagac gggaaaaaat
    12481 ctgaataaaa attattcaaa taactccgcc tggttactct ccttactttt cacccactcc
    12541 atttctgtag acttactata gtctgctaat ttggttccca ctgttttcca gcctgtcact
    12601 tcagcaatct taacaatctt taattttgct ttacgtatct gggcccctct tccctgctgc
    12661 atcgccaata ttggttcctc atcagttgat acagcttcta cataattgcc atccccctct
    12721 tttatgaaca tgaattttgt cttcaatgta ctggtttcaa ttttgaatcg cttcaccatg
    12781 tactgcttct tatcattatc aacatataca gctgtgatta ctttctccgg gtcaaatttt
    12841 ccgatataca gtactttttc ggggtcaatc ttttgtgtca tttcctggtc agtcacttca
    12901 taatttccat cgttgtaaat aacaaggatc ctgtcatccg gctcaaaact tccgagataa
    12961 gtcccctttt cttctgcact aaggcggcca aatttctcat cgaaccaaag cttccgcccg
    13021 ctaagtgtgg ccttccctgc ctctttaaat ctcactgatt tgatgggata ttttgttacc
    13081 tggtttccca tactgctgcg gcctttgata gccagttctt caaaataaaa atcaaattcc
    13141 ttgatgcggg cagcacaacc tggtgttaaa acgattttca ctacttctgc ctcaccattc
    13201 ggatttacac tcaagtaatg aatcttactt ttttcgtctc cctttgtgag gtcatactct
    13261 ttatctcttg taacaccagt tacattaaaa cgttttgcat aactaagacc accctttcca
    13321 tcagaataaa tcatattgta tgtagtacgt tcatcatttt tctgaaaaac agcagcatgc
    13381 aaaatatcct taccgataaa ggtcttatct gagaccttca ccactttcat tattcctcgc
    13441 tttgcaaaaa caataatatc atcgaggtct gagcattcaa acaataattc atctttcttg
    13501 agtcctgttc cgatgaatcc ctcttccctg ttgacataaa gtttagtatt cgcaatagca
    13561 acattctttg cttcaattat ctcaaactgc ctgatctctg tctttctttc tcttcctttc
    13621 ccatatttct ttaacaggtt ttcaaaataa gcaactgcaa aatcaataag atttgccaga
    13681 tcatgcttta cttgttttat ttccgtctct aatcccttta tctggttatt caattcatca
    13741 atatccagcc tgtaaatccg acgaactggt ttttcagtca gtttgataat atcttctctg
    13801 tttatatccc ttttcagttg ttttttaaaa gggataaacg ctttatcaat tgattcgatg
    13861 actttatccc atgtctcatg ctttttctcc agttccttgt atatcttttc ttcgaaaaat
    13921 attttttcca acgatgtata atgccatttt tcctgcaatt cgttcaacct gatcttcaac
    13981 tcctgttcca gcaggtcttt agttttatcg acagaaagtt ttaataattc ctgtacaccc
    14041 agaaactgtg gtttctgttc tacgatcaca caggcattag gagaaatcga agtctcacaa
    14101 tccgtaaact tataaagtgc atcgatggta atatccggag aaataccggg agctaaatca
    14161 acctgtatct ctacatcagc agcagtgtta tctgttactt tcttaatttt aatttttccc
    14221 tggtcatttg cttttacaat agactccatc aactgagttg tggtaacgcc ataaggcaca
    14281 cttcttattg ctaatgtctt cttgtctact tcttcgatat gtgcccttac ttttacttta
    14341 ccacctcttt tcccctggtt ataatcagcc acatctatca ttcccccggt caaaaagtca
    14401 gggtacaact caaatttccg tcccctgaga tattttacag ccgcatcaat cagttcacag
    14461 aaattatggg gaagaatttt tgtagaaagg cccactgcaa taccatcggc accctgtgcc
    14521 agtaataaag gaaacttcat gggcaacgta accggctcat tttttctgcc gtcataactg
    14581 agctgccatt ctgttgtctt tgcgttgaat gcaacttcca aagcaaactt gctgagcctt
    14641 gcttcaatgt agcgggctgc tgctgcatca tcacctgttc tgacatcacc ccagttaccc
    14701 tgggtatcga taagcaggtc cttttgcccc atattcacta atgcatcccc aatactggca
    14761 tcaccatgcg gatgatactg cattgcctgt cctatgatat tggctacttt gttgtaacgc
    14821 ccatcatcca tttccttcat cgcatggagt attcggcgct gaaccggttt taatccatcc
    14881 tcgacagccg gaacggccct ttcaagtatt acataggaag cataatccag gaaccaggtt
    14941 ttatattgcc cgctaaggcc cccatcatta ttttgcactt ctgttatctt attctttgct
    15001 gcgctcatgt taaatccctt tctattgtaa tgcttcttca gttaattctg tttcctgctg
    15061 ttttgttcct ggcaatttta catcaaaatc tttccatcct ttacttacgt caccagttac
    15121 ttcaattttt ccctgctcaa taagctgcct gattcgccac tcaataaaga catctcccgt
    15181 cttaaccggc atccggtgaa gagtattgct tactaaacgc cacgcctttt gccattctgg
    15241 tgtgagattt ttcaggactt ccttatcgta aaatgtatca tcctttgaag caattttttt
    15301 tccaccctct aatattctta caaacccgcc atcgttaact atccgaaccc attcatcagg
    15361 atcaacttca aattcgctaa gggtaatagg tcttgccagt ttttttgctt taataaactc
    15421 ttttggttgt atttcattca gccaggaagg ataaaatata tgtcctttct cattaatgaa
    15481 cggtaagttg ttaagaaaaa gtaccatcac cctgccctgg tagtccttta attgcggcat
    15541 cagccaataa tagccgcaaa catcatgctg gttctgtccc atccatatcc acaattcttc
    15601 atccggattt tcatccagtt ttgacttaag cagttgaact gtttccctgt catcaaagtg
    15661 atgaacttgt gattcctggt aatgtgaata cgataataaa tctttccacc agtttagccg
    15721 tgattgcctt ccctcttccg tgtctaatgc acttaaaggg cctactgcat aatcatccct
    15781 tatctgtatg acatctcctg acattgtttc atctagttca atcacctgtt tcatcagttc
    15841 aacttcgctc tcattaaaaa ctatgtgtat cattttaatt tattatctgg ttgaataata
    15901 taataaacac aggtcgtaag aaaatttctg aagaatggaa ttaatttaat caggccaatt
    15961 tgtttcctgg gcttccaacc gaatttccat tcataaatag ggttaatcaa ataatgtccg
    16021 gtatgaaaaa gtgtatagcc ttctttttta caaatttttt caaaccgttc aatactaatt
    16081 cctgtgtccc gtatttccat catctcttcc acattttcct tcctggttct gagaacccat
    16141 ttgtacattc cttttggcag caggtgaatg tatggcaatc tcgaaattct tgacttgcat
    16201 atctgctgat gtccgccaaa aggcatatac caaggtggaa acccaaagaa aactatccca
    16261 ccaggcttca aaaaattctt catccagcca atcagctttg cctggtcatg tatatgctca
    16321 ataacatcct ttaaaacaat aatatcaaat aatccgttga aatccttctt cacatccact
    16381 tcgtaaatat ctttcactac aaaccgtaat ctgcctgccg ctatatcttc agtcaaaaat
    16441 ttatttgcat ccacaatcct gggagcatcc agttctactc ccacaccttc acatccttta
    16501 ttaataaatg ctttcaatac accaccttca ccacatccaa tctccaggat cctcatacct
    16561 gcctttacag gaaatttttc ttcaataaac ggtataacgt atttctcagc attcaatacc
    16621 tggatgtcaa aatatcgctt cctgtcagca tgaaattcaa acatgtgtta catctttaaa
    16681 caatttcttc tactatatcc aattctactt tcaggttatt aataatgaat tcctgcctgt
    16741 cgggagtatt ctttcccata taatattcca gtaattgctg aatatgttct ccttcctcca
    16801 ccctcatcaa ttgttttcgc attccggcac caataaactg tccaaactca tccgggctta
    16861 tttctcctaa tcccttgaac cgggtcactt caggtttggt tcccagtttt ttcattgcat
    16921 ccagtttctc ctgttcactg aaacaataaa tcgtttcctg tttattccgc acccggaata
    16981 agggtgtttc cagtacatac actttttcat gcttcaccag gtcggggaag aactgcagaa
    17041 agaatgtcat cagtaacaaa cggatatgca taccatccac atcggcatcg gttgcaatca
    17101 ccacattatt aaaccggaga ccttccagtc cttcttcgat attcagtgca tgttgcagca
    17161 ggttgaattc ttcattttcg taaacaactt tcttggtcag gccaaaacag tttaatggtt
    17221 tacctcttaa gctgaatacg gcctgcgttt ccacatttct tgctttggta atagaaccac
    17281 tggcactatc cccttccgta ataaaaatgg tcgtttcttt ttgcttcgct acaaattctt
    17341 cctttccttt tgccggagca tcatcattca ggtgatgacg gcaatccctc agttttttgt
    17401 tatgcaggtt tgccttcttg gctctttcat tagccagctt tttgatacca gccagttcct
    17461 ttctttccct ttcgctttgc tcaatccgtt tttttaaccc atcggcaatt gaagggtttt
    17521 tatgcagata attatccagt tcttttgaga ggaaatcacc aacaaactgc cgcatacttg
    17581 gtccatctat atccacattc actgaaccaa gctttgtttt tgtctggctt tcaaagaccg
    17641 gctctaccac tcttactgaa acagcagcaa caataccagt tctgatatcc gaagcatcat
    17701 aatctttttt gaaaaaatct cttattgttt ttacatacgc ttcacgaaat gcttgctggt
    17761 gcgtaccacc ctgcgtagta tgctggccgt ttacaaagct gtaaatgtct tctccatatt
    17821 cattggtgtg agaaatggca acttcaatat cgttaccggt aagatgtata atggggtaac
    17881 gaatctcatc ctcattggtt ttgcgttgca ataaatccag caatccgttt ttactcacaa
    17941 aacttttgcc attgaagata attttcaggc cggcgttcag aaaacaataa ttccataact
    18001 ggttttccaa aaactcagga tgaaatttga aattcttaaa aacagtttca tcaggaataa
    18061 atgtaaccat tgtcccattc tgttcacctg ttttcgattc tttatgctct tttattaaaa
    18121 atcccctttc aaactcagct gttttttctt tcccttcacg aattgcggtt actttaaaaa
    18181 aactgcttaa tgcattcact gcctttgttc ccaccccgtt caatccaact gatttctgaa
    18241 aagccttgct gtcatatttg gccccggtat taatcttgct taccacatcc acgactttac
    18301 ccaacggaat accgcgacca tagtcacgga tggtaactga ttttccatca attgtcagtt
    18361 cgactgtttt tccatggccc atggtgtatt cgtcaataca gttatccatc acctctttca
    18421 ctaatacata aataccgtca tcaggtgatg aaccgtcacc aagtttacca atgtacatac
    18481 ctggccgaag gcggatatgt tctttccagt caagggattt tatactttcc tcggtatact
    18541 caaaaaggtt ggttgtttct ttatcttttg ccatcgtcag cggtacgttc taagttgtaa
    18601 tggagtgtat ttctttcgat tagcggcttt tgcctacgtt aaggtttggc cattcgggca
    18661 aatataccaa aacgagtcct gcacttctgt tttgtagtta ttaacagatg gggaaaatta
    18721 gccctttatt caaatttaag atggtgcttt aagccgggaa aaaagaacaa tttcaccctg
    18781 ttagccaaag tcataaaagc attttttaaa acaacatcct ttattttttt taaaagcaaa
    18841 gcaaatcaga cttgaactaa ccacgtagaa taaaaacagc gatgtctaaa aaacctgatc
    18901 agagtagttt taaaggtaaa attgaaaacc tgtaatttga taaatttact tgaatatatc
    18961 tttatattat attgattatc atttatttag ataatttaat ttatttttat tataacaaac
    19021 cctccccctg aattatcaat tcactaaatt tgttccctac ttctaattcc atcaaattct
    19081 gagattgccc gactgtgctg gagtgtattt tagcaaacaa tttaagtact gcttttatta
    19141 tctgaaggtt taaaattgag gatttatgta tacgtacgat ctgttagaaa ccgggtgtta
    19201 ctacctggtt aaagagaaag aggagtcccc tatatcgttg attaaaattg cggtagttac
    19261 agaccattgt gtattcgttc agcgcttcaa tgagcctatg gaaacaacat ggaaacttaa
    19321 aaaagacaac ctgcatgata taatagaatg cctcagcgat gaaactgtaa aatcatggga
    19381 agaatattac aggagcaatg aagatgcatt ctatgaagga gatgatgagg agtaatcatt
    19441 ctaactaata accattgatc aaaattttat aaatcctgac tctgtagttt acttgtcata
    19501 ccagttagtt agtagcgttt attcatttta ttaaaatccg tatttccata aacatagggt
    19561 tcaatcccaa attttcccgt tcataaaaaa tgtagcactc tgctaaaata tcttggcaga
    19621 aaaaattact ttagccatac tcaaaaatca acccccatgt ctattttcag gaaaaaggga
    19681 aatgcatgct tatttgtatt ccttttcatt tcacaggtta ttgcggcaca accctcccca
    19741 ccagatgagt tcttacccaa ggactacctg cccgcatcat ttcatgccgg acgaagggat
    19801 gcactcagaa acagtatgcc tgatagttca gttgttgtga tattcgccta ccctacccgt
    19861 actttctcca atgatgtaga gtatatctat catgccaacc ctgatatgta ttatttcagc
    19921 ggttataaag aaccccattc cctgcttatg gtatttaaag aaatgcaaac agattcagcc
    19981 ggatataact ataatgaatt gctatttgta cagaaaaaaa atgcactggc tgaacaatgg
    20041 accggtaaaa gactgggggt ggaaggtgca aaggaaaaac tggggataga aaatgtattt
    20101 aacggtgtcg attttaagaa attcaatatt gatttttcaa aatttaaaaa gatcatttgc
    20161 gacaaaccat cggttgatat tcccaatggt tttgacaacg cagaccttta cgacctggtt
    20221 gaacaattca aacaaaaatc agggatggct gaaaaaaaac aaatgggaag atttgataac
    20281 aggctgtata atgaactaac aggtaaactt cgccggataa aaaccccaga agaattatcg
    20341 ctgcttagaa aagcggttga aatctcctgc ctgggacaaa atgaagtgat gaaagcggta
    20401 catcccaata tgtctgaatt agaaatacag ggattgcatg aatttgttca taaaaaactg
    20461 ggggcggaag ctgttgggta tggatcgatt ataggttcag gagaaaatgg ttgtgtattg
    20521 cattatatgg aaaatataaa aaccaaaata ggtacttcta tgatcctgat ggatgtaggt
    20581 gctgagtatc atggttacac agccgatgtt accaggactg tgccggtaga tggtaaattt
    20641 tcagcagaag aaagagccat ctatcaattg gtatatgacg cacaggaagc tgcgttcagt
    20701 acactaaaag atggttccaa atggtcagat gccagcaatc aagccaggga aataattgca
    20761 aacggcctga tgcaactggg catcattaaa gagaaaagtg aagtgggtaa atattttccg
    20821 catggattgg gtcatcatat cgggctggat gtgcatgaca ggggttccat ggaaacactg
    20881 agaaaagata tggtcatcac tattgaacct ggtatataca ttcaaagcaa tagcgattgt
    20941 gataaaaaat ggtggggtat tgccgtacgt attgaagatg atcttttaat tacagaaaac
    21001 gggtatgaac tcctttcccg ctatgcacca cgaaaaatag aagagataga gaaaatgata
    21061 gcccaaaaaa gtgctttgga tgattttaaa ttgccaccat tagagtctgg caagaaaaaa
    21121 ggcttctgat aagcaaaaaa ggctgtcagt attgacagcc tttaaacttt tgaaccaatt
    21181 ttaaaatacc tggtaacggc gtaattcctg ctgaccggct acggccccgg ttgtattaag
    21241 cacttcatac tttattaacc cgatacccct ggcataataa gctttacccg atccaatttg
    21301 tgaagtcata tcaatccagg aactggtcgc tgtactgaac atttcaaacc tttcttccac
    21361 aacaatcaca tttgtaaaat tcattgttcc ggttgaagtg gtgaatgaaa cgggaacatc
    21421 cttttgcaaa atagaatact taaaccggat gctgaaagga gttggaggag gcaatgttgc
    21481 aatcccgtca aaagcctgtg aattccagga tgttccaacg ggtacattgt ctttcagaaa
    21541 aatataagaa gcccaaagtg gattatcgta acctaaataa gaaccggcgt caaaccattc
    21601 attatattca cctgttgcaa tatttctgtt gtaatatcca gatgaatcaa gacccggagc
    21661 caccccatct tcctgcataa atatattgaa cgtattactc actgcactga ttgttccgga
    21721 agttgctgtc cggtataaag aatccagggg gtcatcatca atttcatagc tccagttact
    21781 atttgttgtc cgtggaaaat aatcatttga aagggcaggc aaacaatcaa cggtaaaggt
    21841 gcaactgctt gtaccaaatg ttacagtaaa ggtctgaggt ccggcagttg caggagttcc
    21901 ggttccagta agtataatga gttgattggg ccctgcagta actgttcccg ttcctgcaaa
    21961 actaaagccg gtaacggtat tagttgaaat gctgtacaca ccgcccgttg tgacattgat
    22021 ctgtacttgt accgtattgg cgccagttaa agcatcccct actctgtaaa taccatttac
    22081 agtagccgga gtgcaggctg ttggtgcacc acctaaagtg cccactgcag ggctgcttac
    22141 attcacaaca aaactacagg tggaagttcc aattgtcacc ggaactgtat tcgcaccacc
    22201 agttgttggt gtaccggatc ctgtaagtgt tacagcctga acacctgtat tgataaatgc
    22261 accggtgcca ctaaagacca tcccctggta tgtggtactt atattgtatg taccgatagt
    22321 tgttacattt acatttatta caacggtgtt gctggcagta agagctaatc cagtagcata
    22381 actaccgttc acaatggcgc cagtgcaggc attcggtgct ccttccaggg taaacactgc
    22441 tggtccgccg gcacctgctg gtaatacagt aacctgtata tcacaaaatg tactatcaaa
    22501 acttactaca aaattattca caccagctgc aaaaggggtt ccgttacttc ttaaggtcac
    22561 agtattatta cctaaagctg taaatgtacc actggcactg aaataaaaac cattgacagt
    22621 atctgtagta acaaggtagg ttcctgtttg ggttacatta atgtctacag aaatggtgtt
    22681 agttgccgga accaaagctg ttcccgcctc ataggttcca ttcactgttt ttggaaggca
    22741 atcacccgag gcatcacttt gcaggcttcc ctgcgctggt gtattcgctc cttcaaaact
    22801 caactccttt tggcacccga ttatcaccag taaacctaaa gccagtaaat aaacaatttg
    22861 tcttttcata tacggaaatg catttaatcg ttaaaaataa gactattttc tgtacctccc
    22921 tgttgacaca gttttaccag tcggagaatc agcaatgtga cagctcaaat attatgctat
    22981 ttttggaaaa aagatattaa tgagaaacgt tttactgtgt cttttattat tacctgtgct
    23041 ttcctgcagt tcccaaaccg gtgatgaaac gagggttggg gctgatgctt ttgaaaaatt
    23101 aattgccagg aaagatgtcc aggtactgga tgtgagaacg gctaatgagt ttaagggtgg
    23161 tcatatcaaa aatgccctgc aagcagactg gactgacagg catgaatttg cagaaaggct
    23221 taaatacatt gataaagata agcctgtata tgtctattgc ctggttggta gccggagtac
    23281 atccgcagca gactggatga gacaaaccgg tttcaagaat gtgatagaat tgaccggcgg
    23341 tatcaatgcc tggaaaagag ctggtaaacc ggttgatggc agcggttccg aaagacaaat
    23401 gaccatgact gaatacatgg ctgcaatacc tgcagataaa acaatgctgg ttgattttgg
    23461 tgctgcctgg tgtccgccat gtgtaaaaat ggaacctgtc cttaatgcca taaaggctga
    23521 caaaagcctc catttttcat ttttaaaaat cgatgccggt gtgcataccg aactaatgaa
    23581 gtccatgaac atagaaccta ttcctgtttt tatcatttat aaaaaaggaa aagaagtctg
    23641 gcgtaaacag gggatagtca gcaaggatga actggcagca caactgaaat aattatttca
    23701 ataccagcaa tgaaaagaaa aaaatttctt caaaggtcgg cagcagttat tggcagcagc
    23761 ctgctcccct cttttgcttt tgcaaaagaa accggtaaaa aatcgttacg atttgcacac
    23821 ctcacagata tgcacctgaa acctggtctt attccagaga ccggcacagc aaaagcatta
    23881 caccatgtgc aacaactaaa accgaaagtt gatttcatca ttaacggtgg ggatgctatc
    23941 atggattctt tgggtgcaga caagcaaaat actgaaatac aatggaacct ttttaaaggc
    24001 actttacagc gtgaaaacag cctgcctgta tatcattgca tcggtaatca tgatatttgg
    24061 ggatggttta taaaacaaga ccgacccgaa aatgaccggc tgtacgggaa agtatgggtg
    24121 gtagaaaccc tggaaatgaa gaacaggtat tacagtttta caaaagggaa ttggcacttt
    24181 atagtattgg atagtacaca acttaacccc gctggcggat atatagcaaa actggatacg
    24241 ggacaactgg attggctgca acaggaatta gcatcagcac cagccggtaa attcatttgc
    24301 ctcgtttcgc atattcctat cctttccatt tgtgccggct tattttttga caaaacagaa
    24361 tctaacggcg acctgaagat ccagcgaaac ctgatgcata cagacttcat ccaattaaaa
    24421 aaaatattcc tgaaatttcc gaatataaga gtctgcatca gtggccatat tcacctccag
    24481 gataaaattg attatctcgg catcaaatat tattgcaatg gcgctgtttc gggtaactgg
    24541 tggaaaggtg ctttccagga atttgaacct gcctatgcaa ttgttgaatt atttgacaat
    24601 ggcacatcca aaagaacaat gatcaaatat gagcagacaa attaatcgta ttttaattat
    24661 gctttccgct gcattgcttc taagcgaaat gatcattgct caatcaccac atgttgaagt
    24721 actgacattc ggtacaaaga caagcatccg cgggctaagt gttgtaaatg ataatgtggt
    24781 ttgggtaagc ggcagcaatg ggacatttgg aagatctact gatggtggaa aaacatggaa
    24841 atggtcagtg gtcaaaggat ttgaaaagaa tgacttccgg gacatcgaag catttgatgc
    24901 caatactgcc atagtaatga gtgtagatgc cccagcctat attttaaaaa ctaataacgg
    24961 cggagaatcc tggaagattg tctacgaaaa taaaacaaaa ggcatgtttt tggatgcaat
    25021 ggagttttgg aatgaacagg ctggcatcgt tatcggggat cctgtggagg gaagattttt
    25081 tatcgccaga acatttgatg gaggaatcag ctggcaggat attccatacg aaaaaagacc
    25141 tgttgcagac agtggtgaag cctgttttgc agcaagtgga actaatgtcc gtgtattaga
    25201 cagagatgaa gcggtgtttg taagcggagg attaaaatca agactatttt caaaaaaaac
    25261 tccggtgatc ttaccagtca ttcagggcaa agaaacaacc ggggctaact cggtggctgt
    25321 gtgggatacg cataaactaa gaggaggaaa aagaatgatt attgcaggtg gtgactttaa
    25381 cgctgataca tctgttacca aaaactgttt ttataccacc aataaaggaa aaacatggaa
    25441 tgtgccggaa atcgccccac atggctacag gagctgtgta gaatattttt caaagaagaa
    25501 ggtttacagt tgcggactca atggtgttga ttattcaaaa gatggcggaa agaactggga
    25561 gtggataagt aaggaaggat ttcatgttgt gagggctgca aaagaaggaa aaacggtttt
    25621 tctcgccggt ggcaacggga agattgcaaa gattgtgtgg aaatgatttc ataattctaa
    25681 aataaacctt atgcttaaaa aattcattga acaaatgcgt ttaaaagcaa ttgccagaca
    25741 actccgtaaa cccaacggtg caatgggaca taaagttgcc cgaaaaatga atgaggcaaa
    25801 tgaatttctc tatgatttta ctttagatga aatgctgatc gcagacaatc actccatcct
    25861 ggaaataggt tttggcaatg gtaaattctt cgataaactt ttaaaaaaag cagataacct
    25921 gaagataaca ggtattgatt tttctgactc gatggtaaaa tctgctaaag aaaataatcc
    25981 ggctgccatt gccgacggca gactcaccat tacaaaagga aagagcgaca accttccatt
    26041 tgccaataac agctttgaca ggattttctg tatcaatgtt gcttatttct gggatgagcc
    26101 ggataaacac ctgcaggaaa ttcaccgggt actgaaacca aagggaaaat tctgtacaac
    26161 tgtccgtacc aaagaaagca tgcagtatat gcctttcacc agatatggat tccggggtta
    26221 tacagaagat gactggaaat tactttcgca taaaaataac cttacttaca tcaatggtgt
    26281 acttgtaaat gaacctccta tagaacatga aggctccact gtaagaacac aatccctgtg
    26341 ttttataaca gaaaagaaaa gatagccggt tataaatttt taagaagttc ttttacactg
    26401 ggcctttttt tataatccgg ctcaaaaaag cgataagtaa ccgttgattc cttatcaata
    26461 atatatactg caggaacagg gagaaacctg ccattggctc cgttattctt ctctatatcc
    26521 agccctgcat tacggtaacg ggtaactgtg ttttcctcca tttcaaattc cacgtcatac
    26581 gctttcatga tctttaatcc ttcatcgtaa agtatgggat attcggcttt tgtcttttcc
    26641 actgtcacgg ctacattttc cggcttttct ggcgaaacag ctactacttc agctcctttg
    26701 tccctgataa attgcaatga atcctgcaat cttgacagct ctttattgca atatggacac
    26761 cattgtcccc tgtaaaacac aagaaccact ttaccttttt tcaataaatc tttgaggcgg
    26821 atctcattac caaattgatc ttttgcttta aaatcaggtg cttttgaaga aataaataat
    26881 ccttctggtt tttcctgtgc aataacagtg tatgccgaaa gtaaaaaaca tataaataaa
    26941 actgtatttt tcatatatcc gtttatggta taaaagtaaa aaaataatca gcgggacaag
    27001 tcatcattaa cataacatta ttttttgctt aattttacaa aatagtagtc ctaaatctaa
    27061 acgacaaatc atgccaaact cccattccga aaaatacgtt tccaatcttc ttattgggtt
    27121 ttcagcactt attggatttg ctttgattac tttctatgca atttttgaac taaaaaagga
    27181 ttcaagttgg tatttctggg ctatcgttgc agcatttttg ctatgtacgg gtatatattt
    27241 ctgccttatt gctattgtac acaaagtgaa atcagacttc aacagaaggg ccaaacaacg
    27301 ggaacaacaa aaaacattta cagcagaccg gatttaatta ctcaaaattc tttgctggtt
    27361 tagccaaacc tgttactgaa cataaaatat acagccccca tcaggaatgc aaaacttacc
    27421 aggtatctcc aatgaaatgg ctctttcata atccataccg caaaggctgc aaaaaccagt
    27481 aaggtaatta cctcctggat cattttcaat tgaaagagat tgaagccatc cactttacca
    27541 aacctgttgg ctggcaccat caggcaatat tcaaataaag cgatcagcca gctgatcaat
    27601 atcgctttcc ataacggcca gccctggtgc tttaaatgat aataccaggc aaaggtcata
    27661 aagacattgg aagcgaataa taagatgata gtacgcatac gtctttgtta tttaatttct
    27721 aataacctta taaagagttc ttgaaattcc agggagaaca aatagtaaaa gcacctaaat
    27781 catttagaat agaaatccaa tatttgttta catacagcat catttataag gaaaaagccc
    27841 ggcattaccg ggctttcaga tattacaaaa ttttcaaact ttattacacc ctgttcaatg
    27901 ttcattaatt ctggttctgc tccaaattcc tgaaatccac actcactaat ttactcactc
    27961 ccggctcctg catcgttacc ccataaatca catccacagt actcatcgtc attttattgt
    28021 gtgtaacgat gataaactgt gaattctcac tgaactggcg gatcatattg gtgaacttac
    28081 ctacattggc atcatccagc ggagcgtcta cctcatccag gatacagaac ggggccggtt
    28141 taatcaggta tatcgcaaat aataaagctg ttgcagtcaa tgttctttct ccaccactaa
    28201 gctgtgttaa agaagacgga cgtttaccct tcggcttggc tattacatct ataccgctct
    28261 cggcaaggtt ttcaggatta tccaatacga gatcgcactg gtcatcatct gtaaagagtg
    28321 ttttaaacac tttttgaaaa ttattcctta cctggtgaaa cgtatcgagg aatttctgat
    28381 tcgctgtagc ttccacttct tcgatcgttt tcaataaact gtccttggca ttaaccaggt
    28441 ctgtcttttg ttctacgatg aagtcatagc gctttttcat ctctgtgaat gcttcaattg
    28501 ccgtgggatt gacttctccc aggttctcca agcgtttttt catccggtct gctttttcct
    28561 gtaattcttc caccggcact tctcccgatc ttgcttcccc caaaatatct tcgagtacta
    28621 ttctgaattc tacattcagc ctttctttca ttccggccaa ctgtaatttc aattcattca
    28681 gtttgtcttt tatggcagcc agtaaatatt ctatctgttc cttttctttt acctggtggc
    28741 gtatagaact ttctttttcg ttcagctggt tacggaaatt ataataagcc tgatcagctt
    28801 cattaagatt tctttcttcg tcttcctttc ttctcagtaa ttccagcaat gattcttcta
    28861 tctcttttaa cgattgtgct gattgcccaa tattattgcc ggtatcagct aattgtgcag
    28921 tattgttttc aatctgctga cgcaagtcac ttaactggtt gtttttgaaa tccagctctt
    28981 gttttaaccc ggttattttg ctctgctgct ttgttacatt caggttaaat tcattgtact
    29041 ggcgggtagc ttcattgtaa tcagactcag ttagcctgaa agcttcatca gcttcagcaa
    29101 gtttcgccga atttgccaat aataattcat tgaattctgc caattggtta cgtacacctt
    29161 ctacagaact atgggtctgc tgcatttgaa ggttcaattc ttccaggcgg tgctggctgt
    29221 tagtctgcat cgaatgcagg ttttccagtt tgttatgcag cgaaaacact tcattggtca
    29281 gttgctgaat ttctctttcc gtttcccgca cagctgtttc ccgcaggtct tcgttgaagg
    29341 caatcacttc attatgtctt gcctgtattt cagaacgcaa cgcttctact accatatcct
    29401 gggcagcaat ttcgtcctgt aatttttcca ggttcttggc acggccgatc ttctttccct
    29461 cgatcaggcc tacactacca ccagtgagtg aatattttcc ttttacatac ttaccgtgct
    29521 tttcaatcac cacaaatcca ttgctatttt gcagggcatc ttcgctatca gctataaaaa
    29581 catttcctaa aagatgttca gccagttttt tgtatttctc ctcaacttcc accacactta
    29641 atgcaggaat ggcaccttcc aattgatgag aagatacaac agcctcaccc tggtttactt
    29701 tatccagtaa aaagaaattt gctttaccct ttttattctc atccagcagg tgtacagcct
    29761 gcaaaccttc ctgcaggtta ttcaccacat aataactcag gtatggttcc agcacatttt
    29821 ccaacgcagt ccggtattct tcattcacat atagtacatc agaaagaata ggtgaattat
    29881 ggctccagtt agagttgtta tggaggaatt taactgaatc aggataacct tccatcgaat
    29941 caatcatact cttcaacagg tcatgctcat tctttttggc atccagttta cgggtttcat
    30001 ctgccaggtt gttgcgaagg ctgtccaata ccgcctgcgt ttgcagaatc tgttctttag
    30061 tattttcctg gtgctgctgc aattgttcca ggtctacttt tttaataccc agctcttttt
    30121 ctttcagaat tctttcatta tcaagttggt gacgttgttc ttcccgatgt accctctcct
    30181 cttctatctg gtgtatcacc cttagcaaat tctgaacaga tgtatctgcg actgccactt
    30241 tcttttctgc atcaaactgg ctgcgttgta tttgctggta ctcgctgcgg agattgtcga
    30301 gcatacggcg cttctcatcc agtatttccc gtttactgtt aacctcgtta ttcagttgag
    30361 tcagttgttc tcccagtatt tccaatgcct tttcttcatc ctgtacctgc tggttggtaa
    30421 agccaatgct ttcttcaatt cctttcagct ggccttccgc tttttgtaaa aactctttca
    30481 ggcttccttc tttttcctgc aggtgttcaa ggcgctgggc agaaagattt ttatcattct
    30541 ccttgctgcg taccgtttgc aacatatcat tgaaagcatg ctgcatactc tgcaactgtc
    30601 tttctttttc aataaaggat agtttctcct gttccagttc agcttcctgc gttcctatct
    30661 ccgcttcaat cttatgtttc ttatcagttt ccgcatcctg ttgttcgttc agttctttgt
    30721 atgtaagatt gaacccctcc aatgaagcct ttgccagttc aatactgact tcccggtaat
    30781 ctttcttaat ctcatgatat ttctctgcct tctttgcctg gctttccagt gctttcagct
    30841 ggttgttgat ttcaaaaagc aggtcctcaa tacgggccag gtcctgctct gtagcatcca
    30901 gtttaagttt tgcctctttc ttacgggttt tataaataga aatacctgct gcctgttcca
    30961 gcattttacg gcggctgttc tccttatctt taatcaggtc atctaccatc cccagttcta
    31021 tgatagcata tgaatcggta ctgataccgg tatccataaa caggttctgt atgtctttca
    31081 ggcggcactg gacatcattg aggcgatact cactttcacc gcttttataa aattttcggg
    31141 ttaccgtaac agtgctgaac tctgttggta acaggttacg ggtgttttca aaagtaaggc
    31201 tcacttcggc aaggccgctg gcacttcggg ttttggaacc attaaataca aggctgtcca
    31261 ggttctcact ccgcaggtgg ctgatcttct gttcacctat cacccagcgg atactgtcta
    31321 caatattaga tttaccacag ccatttgggc ctacaatgcc ggtgatattg tcatcaaaac
    31381 tcacaatggt cttgtcggca aaactcttaa agcccttgat ttctaaactc tttaatctca
    31441 ctgttatctg tttaagtaat tgttaagtca gcggcgaatt taagctaaag gcctataaaa
    31501 gaagatttgg ctaacaggca gttattcaca atgatgggtt aaaaatgtgg gaagaaagta
    31561 gttcctgaaa agcaggacaa aaactggaca aacccgggct ggttatccac gggttttatt
    31621 gtataaacaa aggcaggcag gaatgtttta tctgctctgc cagtaatgaa gattgaagcg
    31681 aaacgaaaga tggtaaaata catttttcct tagttgccta ttttcctggt tgattaaagg
    31741 acttcgactc catctgcaaa ttatccattg cactaagtag ccggaaaatg tagcgaaact
    31801 aatgatatcc gtgaataagg ggtaatgaaa taaaaaagtt tgctgaactg ttgccaggct
    31861 gcaaactttt tgaaatactc tttttaacga taaaccacta ccagctttgg tctgcgggca
    31921 gcatcaatgg cttcactact ggaaaaaatc acatttctgt atatcgcttc attctgaagc
    31981 gtaaggcaaa aaccatagtt actgtttgtg gaaaccatgg ttctcactat ttcagtaaca
    32041 tcaatatctg ttgcattaaa attccactgc gtagttgtcc cgggaattga ggcctggccg
    32101 gcatctgcgg ttcctggctt attattccag gtaatggttg cttcatccca gttcgaggtc
    32161 acccttttta tccatccggg gttatccggt cctgaaccgt aaggagaacc tgggaaatgt
    32221 gaattaccct ccggtgtgaa tccgctgctg gcaacaccat acaatgaaag tttggcagac
    32281 aatatctctg cactggcagg tatggatgaa actgcagaaa atttgatata agaacgcatc
    32341 acaccttcac caaaaccctg tccgccgtat gtccatttgg aataaaccaa ttcagtatgc
    32401 tgacctatgt taagattagg agatgtatta tcaccgtctt tggtaataac caatgcatcc
    32461 tgtccgtctg agccaggttg tattgtgaca gttgttattt gagccggcaa aattattaca
    32521 gacactgtat ccaaaccggt tgcaccgtca tcgtccacca ccatcagttg aaaacgatac
    32581 acaccagcaa tcatacctgt tactcttgta gtagcagcac cgggtgtatg gatagcgggg
    32641 atattcgggc ctgcaacttt gctccacaaa taacccataa tagttccgtc ttcatccgtt
    32701 ccgtttccgc taagtattac agaatcggtt tgcaactgaa ttgtctgagc aggtcccgcg
    32761 tcagcaatgg gcaccttgtt taatttgatt ttcttgcaac cggctgccag aattagaaac
    32821 agagaggcta ccacaaagag attagctttc attagattat tttataattt tcatagaata
    32881 acaaaactta ttacatcttt tcttagccag gtaaaattaa ccagatattt aggaaaaaaa
    32941 actttta
//
LOCUS       DAORTI010000025        32753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_915_length_32753_cov_8.448131, whole genome shotgun sequence.
ACCESSION   DAORTI010000025 DAORTI010000000
VERSION     DAORTI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 32753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32753
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_32753_cov_8.448131"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLO70_05325"
     CDS             complement(<1..1275)
                     /locus_tag="PLO70_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73912.1"
                     /translation="MKPFGILIISLLIIFFSCRKDSFITSADARVTISADTLKYDTIF
                     VTAGSTYRTLKIINENNQKLRLSSLKLMGGAGSAFKMNVDGIAGWQFSNLEINANDSL
                     YVFVQVNVNPNTANLPFVIRDSIEVSYNGNDKLVQLEAWGQNAHFFRDRVITTNETWN
                     NDLPYVILGSLLVNTNQTLTINKGCRIYVHADAPIIVDGSLQVNGLKDTADRVYFQGD
                     RLDEPYKNYPASWPGIFFQSTSKDNILNYTSIKNAYQAIGITAPSPNANPKLTLNECI
                     IDNAYDAGIISLNSSIRARNCLISNCGKNIFLALGGDYQFTHCTVVTYANRYIDHRDP
                     VLTVSNFANNSSNNLNAIFRNCIFWGENGIVNDEVVVLKNGSTIFNVEFDHNLWKVQT
                     IPSNITSNQIINNQSPQFDSINTSRNYYDFRLA"
     gene            complement(1306..2004)
                     /locus_tag="PLO70_05330"
     CDS             complement(1306..2004)
                     /locus_tag="PLO70_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQZ73913.1"
                     /translation="MLTVKEIKESVLHLFFPHICTGCGSDILNVESALCMRCVDAMPE
                     TNFELHPDNPVEKSFWGRLPLIGATAQFYFTKESLMQHLMHQFKYKGNRELGIQLGRM
                     MGEQIMNSGRFHTDALVPLPLFPAKEKRRGYNQATVLCQGMAERMKIPILDKAITRPQ
                     HTETQTKKGRIERWKNMEGKFILSDPNAIKNKHLLLVDDVVTTGATLEACGNELLKAE
                     NVRLSLATLCTATR"
     gene            complement(2004..3377)
                     /gene="radA"
                     /locus_tag="PLO70_05335"
     CDS             complement(2004..3377)
                     /gene="radA"
                     /locus_tag="PLO70_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQZ73914.1"
                     /translation="MSKVKTSFFCQNCGYESVKWVGQCPSCGQWNTFVEELTQKDSKK
                     NDNSWKDYNDEKRTNRTIPLHAIKSSEEKRLPTSDTELNRVLGGGIVPGSLVLVAGEP
                     GIGKSTLFLQNGLWLKDIPVLYISGEESEQQIKMRADRLGLKNENFYLLTETSTQIIF
                     QEIKKLKPQLVIVDSIQTLQTPFIDSSPGSVSQIRECAAEFQRFAKETNTPVFLIGHI
                     TKDGSIAGPKILEHIVDTVLQFEGDRHYAYRILRTLKNRFGSTAELGIYEMTDEGMKA
                     VLNPSEILITQKEDQLSGIAIAATIEGMRPLLIEVQALVTQSVYGTPQRTVSGFDLRR
                     LQLLLAVLEKRGGFHFGMKDVFLNIAGGIKVEDPSIDLAVLCSLLSSYEDVPLPQQIC
                     FAGEVGLSGEIRAVNRIEQRIAEAEKLGFEKIIVSKYNQKGLAKQKFNIEVVTMGRVE
                     EVYKYLF"
     gene            3883..4578
                     /locus_tag="PLO70_05340"
     CDS             3883..4578
                     /locus_tag="PLO70_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQZ73915.1"
                     /translation="MSNEIFLVSLVFLGISFLVSAILKSKFTKYAKIPLSNGMSGKEI
                     AEKMLRENGIYDVKVTSVEGFLSDHYNPVNKTVNLSADVYNGTSVSAAAVAAHECGHA
                     VQHATAYGPLVFRSKMVPAVQVSSMLVNWILLAGMIVLATTKNPTVLLVGIIIMAVTV
                     LFTLVTLPVEFDASKRALAWLDRTNITNSTEYPKAKDALKWAATTYVVAALAAVVTLI
                     QYIMIYMGSRDRS"
     gene            4698..7742
                     /locus_tag="PLO70_05345"
     CDS             4698..7742
                     /locus_tag="PLO70_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ73916.1"
                     /translation="MRKTAGLILAVSLFSVQFLWAQTKTVVGKVTDGSGAPLANASIT
                     VKGSRSGTASGADGTFSLTVPDNVKMLTVSAVGFETKDVSISGSSILVSLNAGESRSM
                     DEVIVVGYGTRIKKDLTGNIARVKGTEVANTPVANFTQALQGRAAGVFVEAQSGKVGE
                     GIKVRIRGAGSINATSNPLYVVDGIPINGDPNSGSGLAEINFNDIETFDILKDASAAS
                     IYGSRAANGVVLITTKKGKSGKTALNVNVQYGTNKPTGYRGFLNAGEYVDLLREAAIN
                     SDNIEGVDPLDPAQYPGSWLQFAEGRLTRYSGYSNWRNLETNTNWEKLAFNDDSKTKL
                     IDISAQGGNDKTRFFISGGFNDQDGILFGNNFQRISSRFNLDHDASNKVKIGFNFSLS
                     RTIANRVADDNEFYTPMQIVALAPITPVRNQAGVLYDRPTTTYYNPLIELEDSRYVST
                     GFRNIGGLYFNYKFTPGLIFKSEFGVDLQNQSDDRFYGFRTIIGQSTNGYGEATWYRR
                     FNYNTNNYLTFNKIIRSVHNIEATAGMSFQKFNTDLSYVTGEDFPVEALKKLASAGRI
                     TGGSTSASENAIVSYFARANYKFDNKYLLSLSGRVDGSSRFGKDNQYGFFPAASAGWV
                     LSEENFLSSSKTISFLKLRASYGISGSDDGFGDFGSLGLWGAAKYNNVSGLVPTQLPN
                     PGLKWERSEQTDIGLDFGLFNNRLSGEIDYYVRNTRDLIYSVPVPGTSGYTTQLTNVG
                     SMQNKGFEFVLNSVNISSRDFKWNSSLNLSKNKNKITKLDGEQTLIPGNDGRYLNSLI
                     VGEGIGVFYGPKFAGADPANGDALYYLADGKTTTNDYNDAASFVVGDPNPDWIAGLNN
                     NISYKGFELSVLFQGVFGNEILNGAGGFMSASFDWFDNQTRDQLKRWQNPGDITDVPQ
                     LRLGYGNGIGASSRYVEDGSYVRLKNLTLAYNLDSKLLSKLKIRSARFYLTGVNLATF
                     TKYTGWDPEVNTDYRANSRNQGGDFYAAPQIKSLTFGINLGF"
     gene            7763..9109
                     /locus_tag="PLO70_05350"
     CDS             7763..9109
                     /locus_tag="PLO70_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ73917.1"
                     /translation="MKKYISIFMAMAVVASSCDKKLDLLPQQSVAEEVALSSDANVKK
                     VLNGAYDAMSSGNLYGGNVLLYSELLAANGEIRWEGTFNQPREVWLKAILTTNSFVRD
                     TWLSAYNTINIANNVLTGINVVNTADQNRVKGEALFIRGAMYFELIRLFAKPYSAGSV
                     SAAPGVPLILTPTRGINQSSYVARSTVEQVYTQILADLTEAEGLLPNTNTVYVAKPAA
                     SAMLSRVYLQMEKYPEARDAANRSITVATANGKSLASNYAAVFNNSSNSVEYLFATQV
                     SALDGANNMHLYWSIPAYGGRDGDVSIQASHLALYSAGDDRLDLFYSGAGDTRSGKWQ
                     QQYKVLPVIRLAEMYLTRAEANFRAGTSVGESPVNDVNRTRNRAGLGSLGTVTLADIL
                     LERKLELAHEGHAVHDLKRLKLSADGFAYNADKMVFPIPQREVDAHAGVLVQNAGY"
     gene            complement(9170..10723)
                     /locus_tag="PLO70_05355"
     CDS             complement(9170..10723)
                     /locus_tag="PLO70_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQZ73918.1"
                     /translation="MNKELIINAAPQGVEIALLEDKKLVELHSEKSDARFAVGDLYLG
                     KVKKLIPGLNAAFVDVGFEKDAFLHYTDLSPYAKSLLKFTTICMNDKSEQGLDFGKFT
                     IEPEIVKTGKINEVLGGKPNILVQILKEPIAAKGPRLSCELSLPGRFVVLTPFNDIVA
                     VSRKIHSSEERKRLQKIVEAIKTKNFGVIVRTAAEGKNTAELHEDLSALVEMWKSIQH
                     NLKGAVAPTRVLSEQTKATSILRDLLNEDFNKIVVNDKNIFSDTKNYIQRIAPEKADI
                     VSYYQNGSPIFDSFGITKQVKSSFGKTVNLTSGAYIIIEHTEALHVIDVNSGYKSVSN
                     NQEQNALETNLEAAEEIARQLRLRDLGGIIVVDFIDMKLPDNKRKLMEKMEEFMAPDR
                     AKHAVLPISKFGLMQITRQRMKPEMNINTQEVCPSCNGTGKISSTLLLEDEIEKNLSY
                     LITHKNGNLKLVVHPILFAFLTKGWPWNTKMAKWKRKFGQKTRLEQDSNYHLTEYKFF
                     DQHDEEIKL"
     gene            complement(11143..12018)
                     /locus_tag="PLO70_05360"
     CDS             complement(11143..12018)
                     /locus_tag="PLO70_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73919.1"
                     /translation="MKKPQWITIIVAVLLVAAIYTLGKTTPSKKTIPLTDNQQHSEDD
                     GHDHGNTKVSITIDTILLMAKKQLTTDQVVRLNALENSITRGDVKVQQLRVYHQLSHF
                     WKDSAKIFEPYAWYEAEAARLENSEKTLTFAARLFLENLQNDDLEQRRQWKALQAKDL
                     FERSLKINPDNDSAKVGLGACYLFGNISAMPMEGIAKIREVVEKDSTNIYAQMTLVKG
                     TMISGQYDKAISRLQTVLGMQPDNIEAVLLMADMYERKGDKVKAIEWYQKSLSFIKPA
                     DIKSAIEQRILELRK"
     gene            complement(12100..12420)
                     /locus_tag="PLO70_05365"
     CDS             complement(12100..12420)
                     /locus_tag="PLO70_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQZ73920.1"
                     /translation="MRKADLVNQISEKTGIPKVDVLVTLETMFKEVKDTLAAGENIYI
                     RGFGSFITKKRAAKIGRNIKKNIAVHIPEHYIPAFKPAKEFVAEVKKLKEPKPDAGPG
                     EEAE"
     gene            12576..13673
                     /gene="mutY"
                     /locus_tag="PLO70_05370"
     CDS             12576..13673
                     /gene="mutY"
                     /locus_tag="PLO70_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQZ73921.1"
                     /translation="MKNNSREQAQKTFVPTLLRWNKEKNTRQMPWKGEKDPYKIWLSE
                     IILQQTRVEQGLNYYNNFLKTFPDIHKLAKAADEKVFKLWEGLGYYSRCRNLLVTARY
                     ISKELNGKFPDNYEDIRKLKGVGPYTAAAISSFAFNLPYAVVDGNVFRVLARIYGITI
                     PIDSTDGKKYFATLAENLLDKKQPGYYNQAIMDFGAVICKPVTPLCSNCGFKKICSAF
                     INNKINELPVKEKKISIKKRWFYYLVLEYKNEIAVRQRIEKDIWQDLYEFPLIEADKE
                     MDKKNILKQAEKNKWVHKGKYELLAVSPLFKQQLSHQLIAGQFIKLKLKGNHDLESNW
                     IWVTKSRIGKFAFPRFINQYLNEKGGQQSLF"
     gene            13785..14213
                     /gene="ssb"
                     /locus_tag="PLO70_05375"
     CDS             13785..14213
                     /gene="ssb"
                     /locus_tag="PLO70_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQZ73922.1"
                     /translation="MRGVNRVMLIGNLGKDPDVQHLEGNIAVAKFPLATTETFKDRTG
                     KLVSQTEWHTVVLWRGLAELAQKYLHKSSLVYIEGRLRTRSWDDKDGNRKFATEVVGD
                     NLIMLDKRMDGGSSTGDGGGMEGMGGSDIPIGDPSENLPF"
     gene            14328..15668
                     /gene="gldE"
                     /locus_tag="PLO70_05380"
     CDS             14328..15668
                     /gene="gldE"
                     /locus_tag="PLO70_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HQZ73923.1"
                     /translation="MESHSIVIPLIDNLSVFLLAVNPQGTTFLGVTLLVLLLLTFAIS
                     GSEVALFSLNKKDLNMLKTKQHASAKRIVTLLEEPKEVYASLLIAGTFVNISIIVLSN
                     FLLSELISFDKIGSLLEVLFKVIIIAFVLVFFGKILPKVWATQNNLRFAYDWSFVVEA
                     LHLLLRRISKWIVKLADGIGKRVGADKSDVLSMQELDEAIDIKSDEEASPEEKNIMKG
                     VVKFGNISVKQIMRSRLYVNGISYNSTFSDLIKRVEELHYSRLPVYKGSLDEVVGILN
                     TKDLIPYLNEGENFDWHPLMRQPFFVPEPKLIEDLLKEFQQKRIHFAVVVDEFGGTSG
                     IVTMEDILEEVIGDIKDEFDDEESDNYKLDDNNYIFEGKIMIHDMCRIMKLPMNTFDN
                     VKGDSESLAGLLLELAGELPTVDWVIPCGDFEFAIMQADNNRIQLVKVTIKQKS"
     gene            15673..16278
                     /locus_tag="PLO70_05385"
     CDS             15673..16278
                     /locus_tag="PLO70_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73924.1"
                     /translation="MINKLKKCFYISLTGYLFFLLSCNSDYSAGKKKGYFKIDFPEKK
                     YQLFEQPGYPYSFEYPVYANVIKDTTFFEDKAGDWWINIEVPRFNGRIHVSYKAINDQ
                     NNFDSLVRDGFKMAYKQHVDVATGINDSVIRTPNGVEGIYFSLGGNTATANQFFLTDS
                     VKHFLRGALYFNAAPNADSLGVVNDFLKKDLQHLINTLKWK"
     gene            complement(16325..16738)
                     /locus_tag="PLO70_05390"
     CDS             complement(16325..16738)
                     /locus_tag="PLO70_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73925.1"
                     /translation="MKSILYAGAALMIGASIYGFVDYSQASNKKEFNDMYTVEKEKPA
                     VATLKTDNDQPTKQAIRKEDKTGEKKNVTPVNIKAKKEDKSEKYADEKKKEYKVKKGK
                     RKLSTKLFSRAPLREEMEELPAQKNETKKTERKDQ"
     gene            complement(16779..17453)
                     /locus_tag="PLO70_05395"
     CDS             complement(16779..17453)
                     /locus_tag="PLO70_05395"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HQZ73926.1"
                     /translation="MQNTRIITLQKSVKVFAQMLFWLPVTVFSLLIIYNTLPYFSFSK
                     DFDFIEERSILFKGSFYNACFYIHIAAGALCIGTALIQFSRYVLKRSKAIHRVSGKIY
                     VSVVLFLGAPTGLYMSFFAKGSFWERSLFMFMAGWWFVTTLYGLSTIHKRNIIAHKVC
                     MIRSYAMAMTAVTFRVYHILFYLLGWDHLENYELSLWISVIGNMFFAEWVIWRQSKQY
                     IKSFAT"
     gene            17625..19385
                     /locus_tag="PLO70_05400"
     CDS             17625..19385
                     /locus_tag="PLO70_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ73927.1"
                     /translation="MAASVVILAQEAAAVDSLKIALGKVTTITEKVNILDELSRVLMN
                     VNPKEADEYGNKLIAVAEESRDRKLMFKAYLSNGTRCSYFASQKAYSQKSIEFYKKAL
                     ELARQNRMEEEVGAAQMHLSTVYRLIPDNDKALSYATQAFSLVSTLRNDSLRAEAHNV
                     YGQAYQAKNENILALRNYLSALRIAEELKNPSLILNCYQYLSSFYSEIEDYDKAIDYM
                     TMAYKKLDDTREKNAPYQKVIFNNYIGNLYAYKKNYDMAISYFELSIRMADSLKFSTL
                     KIPAYVSLLNQYLRIDEPKKALDYLNSPPGDNLKKYLGNFGMASMIDQAYGVVYTELG
                     NYDSARIYLERARPMFENSSNENNKVNFYTQLASFYRKSGDKEHAIEYYLKVKEFSEK
                     NGLLENVKRASRYLDTLYRQSGDIATASIYNGIYYQYKDSIETLKREKELTQVEAADE
                     QQRQLRAVKEKEEKDRQRNNIQYLAITIGIVALFIGLVVLGMFKVSANTIKMIGFFAF
                     LMFFEFIFLIFKKNIYSITKGEPVKDLLFMIGLAALLLPLHHWLEHKVIHYLTSHNRL
                     TSAGHHIKRRLFRRTKEGDE"
     gene            19421..20023
                     /locus_tag="PLO70_05405"
     CDS             19421..20023
                     /locus_tag="PLO70_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HQZ73928.1"
                     /translation="MIVIDNILISEDIVNKKFVCDLAKCKGGCCEDGAAGAPLSKEEL
                     QLVSDLYEKIKPYLTKDAVAEIERKGKYVYDTEFEWVTPTLDSDNEICVYGIRGQDGI
                     IKCAFEQAYNDGVISWKKPISCHLYPIIAKKGKHGDYERVNYEPREQMCSPACVLGEK
                     LKVPVYQFLREPIVRKYGEEFYTALDTIAKGEWEEIDFKE"
     gene            20057..21436
                     /locus_tag="PLO70_05410"
     CDS             20057..21436
                     /locus_tag="PLO70_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HQZ73929.1"
                     /translation="MKETAADFIARSTIKAADKEHRRKINFNIGKYNAVVPQGKLQFT
                     DVHLARERAKNIKWKAIETLDKQLETFESAITRRGAKVIWAEDAQQALDAIQKICEEK
                     NCKTLVKSKSMVTEEIHLNDFLEKNGIESVETDLGEYIQQLDGEPPYHIVTPAMHKSK
                     EDVAKLFAEKLGTDPKATPEQLTLTARRILRDKYVQAEVGLTGANFIIADIGAIAVTE
                     NEGNARLSCSMPKTHIVIAGIEKVIPSMTDLALFWPLLSTFGTGQKITVYNTIITGPR
                     QANEADGPEDMYVILLDNGRTNILANEKQRESLYCIRCGACLNACPVYKNIGGHTYAA
                     TYSGPIGSVITPHLKNMDEWKHLSYASSLCGNCTEVCAVKINLHELLLENRHEAVEEG
                     FSSFTEKMAWKMWKQGMLHRKWMNMAGGNIKNRVVNGLIKGWKEYRGDMEFPKKSFNE
                     LWKEKYGNR"
     gene            21450..22772
                     /locus_tag="PLO70_05415"
     CDS             21450..22772
                     /locus_tag="PLO70_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ73930.1"
                     /translation="MILFANTITHRLKYICDFIGTEITGESIRITDNVLSFAEHAGPK
                     INYSDERLTQEELWIQPYLLLFEKNIHELQIDCFEIKGYKAFFKTNGDFPFDIFAASF
                     YLLSRYEEYLPHQKDMYERYAYENSLAYKAGFLNKPLVNIWLQEFKALLQAQFPALST
                     RSSTFNFLPTYDIDEAYSYKHKDWKRSAGAAIKDLLRRDWRRFRLRRKVLNNELADPF
                     DSYEWIDNLHRPYQLKPRYFFLVAEQTGKFDRNILPKEIALQTLIRQHADKYDIGIHP
                     SWQSGDDSLLIQQEKERVEHIIKKKIVASRQHFIRFTLPETFRHLIESGIKKDFSMGY
                     GSINGFRASVASPFYWYDLGTEQATYLLLYPFCYMEANSFFEQKYSSQQALAEMRYFY
                     NEVKKVNGTFITIWHNTFLGTDKRFSGWRDIYARFVNEIHHQGGRKLL"
     gene            complement(22769..23779)
                     /locus_tag="PLO70_05420"
     CDS             complement(22769..23779)
                     /locus_tag="PLO70_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQZ73931.1"
                     /translation="MNFFDFHKTIVQSSADSLKKLAVFNPLTIHRKGTKAKALFALAL
                     VCVLWGTTWIASKEGVRHMPALQLAGIRQFIAGLIYVAFFLIKGASLPKGKEWIPVLV
                     LSFLNFIMSNGLSTWGVKYISAGLGSIMGAIFPLWLVVIGLFASREKLPQKAIIGLLL
                     GFSGVCIIFYEHLEDFFIPDFRFGIILSLIATWTWAFATLYTKQQAASFNPYFSLGLQ
                     MLISGITLISFTSVTGNAVSISQIPWESWTAIAYLIIFGSLLAFIAYLYALQNLPTEQ
                     ASIYAYINPIVAVLCGWLIFNERITIFITVGGLVTLLGVYLVNKAFKAVPPPEQPETE
                     GI"
     gene            complement(23779..25284)
                     /gene="purH"
                     /locus_tag="PLO70_05425"
     CDS             complement(23779..25284)
                     /gene="purH"
                     /locus_tag="PLO70_05425"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQZ73932.1"
                     /translation="MNKKIQSALISVFYKDGLETIVQQLSELGVTIYSTGGTQTFIEK
                     MGIKVVPVEDLTTYPSILGGRVKTLHPAVFGGILGKRDDTTHLQEMKQYNIPEIDLVI
                     VDLYPFEETVASTTDEKAIIEKIDIGGPSMIRGAAKNFRDVVVIARKADYDEFSKLLA
                     FQKGETSVEQRRQFAIKAFDVCTAYDTAISNYFHQSAIETPFNKPQKTMRYGENPHQS
                     GVFYGDLNKVFDQLNGKELSYNNLVDVDAAVQLIHEFSDTTFAIIKHTNVCGVAQRPT
                     VKGSWDAALAGDPESAFGGVLVCNDTIDKATADAINEIFFEVLIAPAFDEDALIVLKS
                     KKNRILLQLKAQPGNKVQFKSVLNGVLVQSVDEGNFTNWTETVKRKETETEKQDLIFA
                     NLICKHLKSNAIALVKNKQLIGKGCGQTSRIDSLRQALEKAKQFDFDMHGAVMASDAF
                     FPFDDSVRLGHEAGITAFIQPGGSIRDNDSIEYCKTHNLAMVMTGMRHFKH"
     gene            complement(25337..27430)
                     /locus_tag="PLO70_05430"
     CDS             complement(25337..27430)
                     /locus_tag="PLO70_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQZ73933.1"
                     /translation="MASYGNRFWKKTPFLKLLIPLMLGIIVQWHLQLYANTWLIILAV
                     GITGLCSFFFIPVLTRFHLSTFSGISTGTTFLSFGALLVIQNDIRNNGQWLGAVYKDK
                     DAIIATLDEPPVEKTKSNKANASVSFLIREGKINPAQGKIIIYFKKDSAGQQFSYGSQ
                     VIFKKSLQEIKNSGNPGGFDYKRYSLFQGITHQVFLKPGEFEIIPGKKENWLKKLIFT
                     SREKVLTILRQNISGEKELGLAEALLIGYKDDLDQSLVQSYTNTGVVHIIAISGLHLG
                     LIYWLLVQLLKPLRRNRKTRWLSPVLIIAGLWSFSLLAGAQPSVLRSALMFSCIVLGE
                     SFTRKTTIYNTMAVSAFILLCYNPFWLWDVGFQLSYAAVLSIIIFMRPIYNWFNIKNK
                     ILDFVWKLNAVTLAAQILTIPLGIYHFHQFPTSFILTNFLAVPLSSIILFGEILLCIV
                     SLIPAVAALAGKILTSLIWLMNTYVERIELIPFSLWDGLQLSITQAILLLFFTAGISY
                     WLMERSTNGLKIGIVSLLLFTAERTVSFIAAEKQQKIIVYNVPQKKAIDFIDGRNYLF
                     IGDSGLLANDFARNFHLKPSRVLYRIKGADTLNDFLGYKNYISYKKRYILLLDETISF
                     TPQVNKPIIDLVVISKNPKLYIAKLAGSLDIKQVVFDGSVPAWKTKYWKKDCDSLRIP
                     WHDVSIKGAFVMNLN"
     gene            complement(27483..27953)
                     /locus_tag="PLO70_05435"
     CDS             complement(27483..27953)
                     /locus_tag="PLO70_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQZ73934.1"
                     /translation="MNKETQSIIRRIENVNSGEPWFGRAVFVILEEVDAKKVSIKPNC
                     TEHSMLELLYHMITWADFTLKRLEKNNKMDMAAFEKMDWRELNPKLHSWKKALAEFKA
                     INKKIIALLDKKDDDFLLEIVDYRKYNYRFLLNGMIEHSIYHLGQVAYLNKMLS"
     gene            complement(27972..29084)
                     /locus_tag="PLO70_05440"
     CDS             complement(27972..29084)
                     /locus_tag="PLO70_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73935.1"
                     /translation="MKKAILFLLVIIAYVNVLAQNVGIGTTTPVSKLHVKGTGSGTQI
                     VLEENAGSILRISNEPSGTGPYIGTTTNNPLSLVTNNSAKLSISTSGNIGIGQPSPTS
                     PLHFSNTLGNKIALWGTNPNHYGIGIQAFQMQFYTPANTDDFVFGNGNSSSLIETMRI
                     KGNGNVGIGTNNPQTTLDINGDVNIENKLLLNNSAGNNGQVLVSKGNGAAPNWQNIAY
                     SNNDRFYYTLADNQRGTSGLFSQTILYNTVYSNSSAISYNNSNGTFNVNKSGLYSINA
                     DFAPYTDKTVENASVLCILDLNASGFTENFYTGGLDYMGIFGSGHTYSSTIRKKWEIY
                     LVAGTQFSFFITILTNNQNASGLQSKTGLSINLISE"
     gene            complement(29293..32118)
                     /gene="carB"
                     /locus_tag="PLO70_05445"
     CDS             complement(29293..32118)
                     /gene="carB"
                     /locus_tag="PLO70_05445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQZ73936.1"
                     /translation="MPKDNSIKSVLIIGSGPIIIGQACEFDYSGTQAARSLREEGIKV
                     ILINSNPATIMTDPMMADRVYLLPLTVESIEQILQENEIDAVLPTMGGQTALNLAKEA
                     EDLGLWKQYNVRLIGVDIKAIDKAEDREKFRQWMIALGVPVATAKTANSFLEGKEFAQ
                     QIGFPLVIRPSFTLGGTGGGFVHDKDDLDEALNRGLQASPIHEVLVEKAVLGWKEFEL
                     ELLRDSADNVCIVCTVENFDPMGVHTGDSITVAPAMTLSDTGMQLMRNTALMMMRDLG
                     NFAGGCNVQFALNPDTEEIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LENQITKTTSAYFEPALDYVIVKIPRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTE
                     AIQKACQSLENNAVGLGYYGKSLMHADELLEYIKTPKWDRIFRIKDALMAGISVGTIS
                     KATNGIDRWFLYEIQKICNLEKEIAKYDLADLPIELLREAKVNGFSDEQISRITEHGD
                     EDDVYKKRKEAGITRVFKMVDTCSAEFEARTPYFYSTFESGNSNESKVSDKKKIIVLG
                     SGPNRIGQGIEFDYCCVHGLLAIKECGYESIMVNCNPETVSTDFDMADKLYFEPVYWE
                     HLWEIIEHEQPEGVIVQLGGQTALKLAEKLHQKGIKIIGTSYDSMDIAEDRGRFSDRL
                     KELDIPYPEYGTAEDVDEAIAVANKVGYPVLVRPSYVLGGQRMRIVLNDEEVEKAVVS
                     LLKHLPGNKILIDHFLDRCQEAEIDGIFDGNDFHVMGVMEHIEPAGIHSGDSNAVLPA
                     FNLTPLIVHTMEEYAEKIARALHIKGLINIQFAIKDGKVYVIEANPRASRTTPFIAKA
                     YQIPYLNIATKIMIGTHKLKDFTFEKKLKGFAIKEPVFSFNKFPGVNKELGPEMKSTG
                     EAIRFIKDLRDPYFRQLYKDRSMYLSK"
     gene            complement(32233..>32753)
                     /locus_tag="PLO70_05450"
     CDS             complement(32233..>32753)
                     /locus_tag="PLO70_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73937.1"
                     /translation="MVPLEKNPAEPIDSTLIGYWYGIVKDGSDYFGIEALEISRQSDS
                     VYSIIRYGKAVKGDMILPDTSYFTAYTSWIGDQRFMNVTGTITLTEPIGKKNTKTTEK
                     NVFYLSAFEKSNDTLRVKTITESFTTRKIINQPGDLKKLVIEMSGKKNIYDEQYSLSY
                     RKIPKTPHFQSN"
BASE COUNT         9599 a         6369 c         6742 g        10043 t
ORIGIN      
        1 cgccagcctg aaatcgtaat aatttcttga tgtattgata ctgtcaaatt gaggtgattg
       61 gttattgatg atttgattgc tcgtgatatt ggaagggata gtttgtactt tccagagatt
      121 atgatcaaac tctacattga aaatggtaga gccatttttt aataccacta cttcatcatt
      181 tactattcca ttttctcccc agaagatgca attgcggaaa attgcattca gattgttgga
      241 gctgttattg gcaaaattag aaaccgtcag cacagggtct ctgtggtcga tataacggtt
      301 ggcatatgtc actaccgtgc aatgggtgaa ctggtaatca ccccccaggg ccaggaaaat
      361 atttttaccg caatttgaga tcaggcaatt ccgggccctg atacttgaat tcagtgaaat
      421 gataccagca tcataggcat tatcaataat acattcattc agggtaagct ttggattggc
      481 attgggtgat ggggctgtta ttccaattgc ctggtaggca tttttaatgc tggtataatt
      541 caggatattg tcttttgagg ttgactggaa gaaaattccc ggccagcttg caggatagtt
      601 tttatagggc tcatccagtc tgtcgccctg gaagtaaacc cggtcagcgg tatcttttaa
      661 tccatttacc tgcaaactgc catccacaat aataggcgca tctgcatgaa catatatgcg
      721 gcaaccttta ttaatggtca gtgtttgatt ggtgtttacc agcaatgacc caagaattac
      781 gtaaggaagg tcattattcc atgtttcgtt tgtagtaata actctgtccc tgaagaaatg
      841 agcattctgt ccccatgctt ccagctgtac cagtttatca ttcccgttat agctcacttc
      901 aatactgtcc cgtataacaa aaggaaggtt tgctgtatta ggatttacat ttacctgtac
      961 aaatacatat aggctgtcgt tagcatttat ctccaggtta ctgaactgcc aaccggctat
     1021 accatctaca ttcattttaa aagcagaacc agctccaccc ataagcttca atgatgataa
     1081 acgaagcttc tgattattct cattaatgat tttcagggtc cggtaagtag agccggctgt
     1141 tacaaatatt gtatcatact ttaatgtatc tgcactgata gttactcttg catcggcgga
     1201 agtgataaag gagtctttcc ggcaggaaaa aaaaatgatg agcagggata tgatgagtat
     1261 tccgaagggt ttcattattc tcaaaaataa ctgaagtatt tcagattatc gggtagcagt
     1321 acaaagtgta gcaagactta accgtacatt ttctgctttc aatagttcat ttccgcaggc
     1381 ttccagtgtg gcaccggtgg ttaccacatc atctaccagc aggagatgct tattttttat
     1441 tgcattagga tctgaaagta taaattttcc ttccatattt ttccatcttt ctatccttcc
     1501 ttttttggtt tgtgtttcgg tatgttgcgg gcgggtaatc gctttatcca aaattggaat
     1561 tttcatcctt tcggccatac cctggcataa gacagtggcc tggttatatc ccctcctttt
     1621 ttcttttgcc gggaaaagtg gcagggggac caatgcatct gtatgaaaac ggccggagtt
     1681 cataatctgt tctcccatca tcctgcccaa ttgaataccc agttccctgt ttcctttgta
     1741 tttgaactgg tgcatcaggt gctgcatcag cgattctttg gtgaaataaa attgggcagt
     1801 ggcaccaata agaggcagcc ttccccaaaa tgatttttca accgggttat caggatgcaa
     1861 ttcaaaattt gtttcaggca ttgcatctac acatcgcatg cataatgcac tttccacatt
     1921 cagaatatcg cttccgcagc ccgtacatat atgcggaaag aataagtgaa gtactgattc
     1981 ttttatttcc tttactgtaa gcattaaaaa agatatttat aaacttcttc tactcttccc
     2041 atcgtcacca cttcaatatt gaacttttgc ttggctaatc ccttctggtt gtatttggaa
     2101 acaatgatct tctcaaaacc cagtttctct gcttctgcaa ttcgttgttc gatgcggttg
     2161 actgctctta tttctccact taaaccaact tccccggcaa aacagatctg ttgtggtaaa
     2221 ggcacatctt cgtaagacga taataaggaa catagaacgg cgaggtcgat agagggatct
     2281 tctactttaa taccgcctgc tatatttaaa aagacatctt tcataccaaa atgaaaaccg
     2341 cctctttttt ccaaaactgc taataataat tgtaaacgtc gtaagtcaaa accgctgaca
     2401 gtacgttgtg gagtgccgta tacagattgt gtgacaagtg cctgcacttc gatcaataaa
     2461 ggcctcatgc cttcaatggt ggcagcaata gcaataccac tcaactgatc ttctttttgg
     2521 gtaattaata tttcactcgg attaagcaca gctttcattc cttcatctgt catttcgtaa
     2581 atacctaatt cagcggtact tccaaacctg ttctttaatg ttctgagaat ccggtaagca
     2641 taatgcctgt caccttcaaa ctgtaataca gtatctacta tgtgttccag gatttttggc
     2701 ccggcaatac ttccgtcttt ggtaatatga ccaattagaa agacaggagt attggtttct
     2761 ttggcaaaac gttgaaactc ggctgcacat tctcttattt gtgacacact tcccggtgat
     2821 gaatcaataa acggtgtctg taatgtttgt attgaatcaa caatgaccag ctgaggcttt
     2881 aactttttta tctcctggaa aataatttgt gttgaagttt ctgtcagtaa ataaaagttt
     2941 tcatttttca atccaagcct gtcagccctc attttaatct gctgttcact ttcttctcca
     3001 cttatataaa gtacaggaat atcttttaac cataatccgt tttgtaaaaa caaagttgat
     3061 ttgccaattc ccggttcacc cgccactaat accaaacttc cgggaacaat gccaccccct
     3121 aatacccggt tcaattctgt atcagatgtc ggtaatcttt tttcttcact gctttttatt
     3181 gcatgtaaag ggatagtgcg gttggttctt ttttcatcat tgtaatcttt ccagctatta
     3241 tcattctttt ttgaatcttt ttgtgtcagt tcttcgacaa atgtattcca ctgaccgcag
     3301 gatggacatt gaccaaccca ttttacactt tcatatccgc agttttgaca aaaaaaagat
     3361 gtctttacct tactcataag ataaaattaa agtatcaggt tcagttgatt gaaaaagttt
     3421 gaaaacgaaa gtttataaac agtcaattat tatctgccgg gacaaaaaat gttttcgaaa
     3481 tgtatgcgta aaggaagtaa aatttgcggt gactatcagg tgtagtttta aaatgcaaaa
     3541 agggccaatc ttacgactgg ccctcttact tactaaccca ttaaattcta ttgctaagtt
     3601 acaattatgc cccacatttc aaaataaatt tttggggctg tgaataactt cctgctccgg
     3661 gattcaggtg ttattttcaa ttttagtaag cataaatatt tgtataaaat aatgataatc
     3721 atattattat attgataatt taaataaagt aaaatgcagt tgaaaattaa tttcaggtaa
     3781 ggatttaagt ttagaggatg acaaaacggc tgaaaaaatc tgtttaacca aaatggtaat
     3841 aaatttgtta ttctttaata ctaaaatgta aaaaaaatga taatgtcaaa tgagattttt
     3901 ctggtttcac tggttttttt aggtatcagt ttcctggttt cagccatcct gaaaagcaag
     3961 tttacgaagt atgctaaaat acccctttct aacggtatgt cgggcaagga aatagctgaa
     4021 aaaatgctcc gcgaaaacgg gatttatgat gtcaaagtga cctctgttga aggtttttta
     4081 tcagaccact ataaccctgt aaataaaaca gttaacctga gtgcagatgt gtacaacgga
     4141 accagtgttt cggctgctgc ggttgccgcc catgaatgcg gccatgcagt tcagcatgcc
     4201 acagcctatg gaccattggt tttcaggagt aaaatggtac ctgccgttca ggttagctct
     4261 atgttagtta actggatact ccttgccggt atgatagttc tggcaacaac gaaaaaccct
     4321 acggttttgc ttgtggggat aatcatcatg gctgttaccg tcctttttac cctggttacg
     4381 cttccggtag agtttgatgc cagtaaaaga gcccttgcat ggctcgacag gacaaatatt
     4441 actaactcca ccgaataccc gaaggctaaa gatgctttga aatgggctgc aacaacctac
     4501 gtagtagccg ctttagcagc tgtggttacc ctcatacaat acattatgat atatatgggt
     4561 agccgcgaca gaagctgatt ttattgcgta aggacaagaa attcaattat tttaaaaaaa
     4621 ccgtgcagca gtttgcacgg tttttttttc tttaacaaat tgttaatttc tttcaccaca
     4681 tatcaaaatt tatgaatatg agaaaaactg cagggctgat actggcggta agcttatttt
     4741 ccgttcagtt tttatgggcc cagaccaaga ccgtagttgg aaaagtgact gatggcagcg
     4801 gcgcaccttt agccaacgcc tcgatcacag taaaaggatc ccggagtggt acagcttcag
     4861 gggcggacgg aactttttca cttactgtac cagataatgt aaaaatgctc actgtttctg
     4921 cagtgggttt tgaaacaaag gatgtgtcta tttcaggttc ttcaatttta gtttcattaa
     4981 acgcaggtga atcacgcagt atggatgaag tgattgtagt gggatatggt acccgtatta
     5041 aaaaggacct gaccgggaat attgcccgtg taaaaggtac cgaagtagcc aatacccccg
     5101 ttgctaactt tacccaggct ttgcagggcc gtgcagctgg tgtgttcgta gaagctcaga
     5161 gtggaaaagt aggcgaaggt attaaagtaa ggatccgtgg tgccggttct atcaatgcta
     5221 cgagtaaccc tctttatgtg gttgatggta ttcccattaa tggtgatcca aattcaggaa
     5281 gtggcctggc tgaaatcaac tttaatgata ttgagacttt tgatatcctt aaggatgcat
     5341 ctgccgcttc tatttatggt tcaagagcag ccaatggtgt ggtattgatt actaccaaaa
     5401 aaggaaagtc aggtaaaaca gcattgaatg taaatgtgca gtatggtacc aacaaaccaa
     5461 ccggttaccg cggtttctta aatgcaggtg aatatgtcga cctcctgaga gaagcagcga
     5521 ttaacagtga taatatagaa ggagtggatc cattggaccc agcccaatat cctggttcat
     5581 ggttgcagtt tgcagaagga aggcttaccc gttactcggg atattctaac tggagaaatc
     5641 ttgaaacaaa tactaactgg gagaaactgg cattcaatga tgattctaaa accaaactaa
     5701 ttgatatcag tgcacagggt ggcaatgata aaacccgctt ctttatcagc ggtggtttca
     5761 atgaccagga tggtatcctt tttggtaaca atttccagcg tatctccagc cgttttaacc
     5821 tggatcatga tgcttccaac aaagtaaaga tcggtttcaa ttttagtttg tcaagaacca
     5881 tagccaacag ggttgctgat gataacgagt tttatactcc tatgcagatc gttgccctag
     5941 cacctataac tcctgttcgt aaccaggccg gtgttctata tgatcgtccg acaacaacat
     6001 actataaccc gctgattgaa ttagaggatt cccgttatgt ttctactggt ttccgtaaca
     6061 ttggtggttt atactttaat tataagttta ctcctggcct catctttaag tcagagtttg
     6121 gagttgacct tcagaaccag agtgatgacc gcttctatgg attcagaacc attattggcc
     6181 agtctaccaa cggatatggt gaagcaactt ggtacaggag gtttaactat aacaccaaca
     6241 actatcttac attcaataaa attatccgca gtgttcacaa cattgaagca actgcaggta
     6301 tgtctttcca gaaattcaat acagatcttt cttatgtaac aggagaggat ttcccagttg
     6361 aagcattgaa aaagctggcc agtgccggac gtattaccgg aggttccact tcagccagtg
     6421 aaaatgcaat tgtgtcttac tttgcaagag ccaactataa atttgataac aaatacctgc
     6481 tttctctaag cggtcgtgta gatggttcat caagatttgg aaaagacaat cagtatggtt
     6541 tcttccctgc ggcatcagca ggctgggtat taagcgaaga aaatttcctg agcagttcca
     6601 aaactatcag cttcctgaag ctgagggcca gttatggtat ttctggtagt gatgatggat
     6661 ttggtgattt cggatcactg ggtctgtggg gcgctgctaa atacaacaac gtatccggtc
     6721 ttgttccgac acagttaccc aacccgggat tgaaatggga aagatcagag cagaccgata
     6781 ttggccttga tttcggattg tttaataatc gtctttccgg tgaaattgat tactatgtaa
     6841 gaaatacccg tgacctgatt tacagtgttc cggttcctgg tacttccggt tataccacac
     6901 aattgactaa cgttggttct atgcaaaata aaggttttga gtttgtgttg aattcagtca
     6961 atattagcag ccgtgatttt aaatggaatt ccagcctgaa tctttccaag aacaagaata
     7021 agatcactaa actggatggt gagcagacat tgattcccgg taatgatggc cgctacctga
     7081 actcactcat cgtgggtgaa ggtatcggtg tattctacgg accaaagttt gcaggagcag
     7141 atccggcaaa tggggatgca ttatattacc tggctgatgg caaaaccact accaatgatt
     7201 acaatgatgc tgccagtttt gtagttggtg atccaaaccc agattggatt gcaggtttga
     7261 ataataacat cagctacaaa ggttttgaac ttagtgtcct gttccagggt gtgtttggca
     7321 acgagatatt gaatggtgcc ggtggattta tgtctgccag ctttgactgg tttgataatc
     7381 aaacccgtga tcaattaaag cgctggcaga atcctggtga tatcaccgat gttccacaat
     7441 tgcgtttagg atatggtaat ggtataggtg catccagccg ttatgtagaa gatggctcct
     7501 atgtaaggct gaaaaacctg acactggctt acaatcttga cagcaagttg ctgagcaaat
     7561 taaagatccg ttctgcccgc ttttatttaa caggtgtaaa cctggcaacc tttacaaaat
     7621 atacaggatg ggatcctgag gtaaatactg actatcgtgc aaatagccgt aaccagggag
     7681 gtgacttcta tgcggcaccg cagatcaagt ctttaacatt tggtatcaat ttaggattct
     7741 aaaaatttaa atcaagatca gtatgaaaaa atatatatca atatttatgg ccatggcagt
     7801 agtagccagt tcctgtgata aaaaactgga cctgctgcca cagcaaagtg tagccgaaga
     7861 agtggcactc agctctgatg caaacgttaa aaaggtgctg aatggtgcct atgacgctat
     7921 gagcagcggt aatttatatg gtggtaatgt gcttctttat tctgagttgc tggctgccaa
     7981 tggtgaaatt cgttgggagg gtacttttaa ccaaccccgt gaagtatggt taaaagctat
     8041 actgaccacc aatagttttg taagggatac ctggttgagt gcctacaata ctattaacat
     8101 tgccaacaat gttttaacag gtatcaatgt ggtgaatact gctgaccaaa atagggtaaa
     8161 aggagaagca ttatttattc gtggagctat gtattttgag ctgatcaggc tttttgctaa
     8221 accatatagt gccggatcag tatctgctgc tcccggtgtt ccgcttattt tgactcctac
     8281 caggggcatc aaccaatctt cttatgttgc cagaagtact gttgagcagg tgtacacaca
     8341 aatactagcc gatctgacgg aagcagaggg cctgttacct aataccaata ccgtgtatgt
     8401 agcaaagccg gctgcatctg ctatgttatc cagggtgtac ctgcaaatgg aaaaataccc
     8461 tgaagcaaga gatgctgcta accgttctat cactgtagct acagcaaatg gtaaaagtct
     8521 ggcttcaaac tatgcagcgg tatttaataa ctcatccaac tctgtagagt atttatttgc
     8581 tacacaggta agtgcattgg acggtgccaa caatatgcat ctatactggt ccatccctgc
     8641 ttatggcggt cgtgatgggg acgtttccat acaggcaagt cacctggcac tatattctgc
     8701 cggcgatgac aggctggacc tgttctattc aggcgctggt gataccagaa gcggtaaatg
     8761 gcagcagcaa tacaaagtat tgccagtaat acgcctggct gaaatgtacc tgacccgtgc
     8821 agaagctaac ttcagagctg gtacttcagt aggtgaaagc ccggtaaatg atgtgaacag
     8881 aacaaggaac agggccggtt tgggttctct tggtacggta acacttgctg acatcttact
     8941 ggaaagaaaa ctggaactag ctcatgaagg ccatgcagta catgacctga aaagattaaa
     9001 gttgagtgct gatggttttg cgtacaatgc cgacaaaatg gtattcccta ttccgcaaag
     9061 agaagtggat gcacatgcag gtgtattggt gcagaatgct ggctactaat attagatttg
     9121 cttatgagga caaaaaaccc cggtagatcc ggggtttttt aataaggggt caaagtttta
     9181 tctcctcatc atgctggtca aaaaatttat attctgttag atgataattg ctgtcttgtt
     9241 ctaatcttgt tttttgacca aatttccttt tccatttagc cattttcgta ttccagggcc
     9301 aacctttagt aagaaaagcg aagagtattg gatggacaac caatttcagg ttgccatttt
     9361 tatgggtgat aagataactg agattttttt caatttcatc ttccagcagc agggtagatg
     9421 aaatctttcc ggtaccattg cagcttgggc aaacctcctg ggtgttgata ttcatctccg
     9481 gcttcatccg ctgccgggta atttgcatca gcccgaattt tgaaatagga aggacagcat
     9541 gcttggccct gtctggcgcc ataaactctt ccattttttc cattagcttg cgcttattgt
     9601 caggaagctt catatcaatg aagtcaacaa caataatacc acccaaatct cttaaccgga
     9661 gctgacgggc aatttcttcg gctgcttcaa gatttgtttc caacgcattt tgttcctggt
     9721 tattacttac acttttgtaa ccactgttta cgtcaattac atgtaatgct tctgtatgtt
     9781 caataataat ataggcacca ctggtaaggt ttactgtttt accaaaagat gattttacct
     9841 gcttggtaat accaaaagaa tcaaaaatcg gtgatccatt ctgataataa gatacgatgt
     9901 cagctttttc aggagctata cgttgtatat aattttttgt gtcgctgaaa atgtttttat
     9961 cattaacaac gattttattg aagtcttcat ttaataagtc acgaaggata ctggttgctt
    10021 ttgtctgttc gcttaatacc cttgttggag caactgcacc tttaaggtta tgctgtatgc
    10081 ttttccacat ttccaccagt gcagaaaggt cttcatgaag ttctgcagtg ttcttacctt
    10141 ctgccgccgt tcttactata acgccaaaat tttttgtctt aattgcttca acaattttct
    10201 gtagtctttt tctttcttct gaggaatgta tttttcttga tacagctacg atgtcattaa
    10261 agggagttaa taccacaaaa cggccgggta gtgaaagttc gcaactcaac ctggggcctt
    10321 ttgctgctat cggttctttc aggatctgaa cgaggatatt gggtttgcca cccagtactt
    10381 cattgatctt tcctgtttta acgatttctg gttcgatagt gaatttgcca aaatccaaac
    10441 cctgttcact tttatcgttc atacagattg tagtgaactt caacagcgat ttcgcatatg
    10501 ggctcagatc agtgtaatgc agaaaagcgt ctttctcaaa tccaacatct acaaaagcag
    10561 cattgagccc ggggataagt ttttttactt tacccaggta caaatcacca acggcaaaac
    10621 gggcatcgct tttttcgcta tgcagttcta ccagcttttt gtcttccagc agtgcaattt
    10681 caacaccctg cggggctgca ttaataatta attctttgtt cattacagct tacataaatg
    10741 aatcacctta gtgctgtaaa caccagttgt caggagtccg tattggaatg cggggttgta
    10801 cctgtaggat atttgcggtt taatatggtg gggtgcaaac gtatacgaca aaacaaccgg
    10861 tggactgaga gattggtgac tagttaactt gttaattagt taatcaggct ttttatatcc
    10921 gggttaagat ttcacttaat aaaatctcaa aacccactta actaattaac aaattaacta
    10981 attcacttac ttgtttctct tcttatgacg gttctttctc agtctttttt tacgcttgtg
    11041 cgtcgcaatt ttatgacgct tccttttttt accacaaggc atactcaaat aatttttaaa
    11101 tcttgtttat aataagttta ctaatacttg gctctttaaa agctatttcc ttaactccaa
    11161 aattctttgt tcaatggccg atttaatatc agccggttta atgaaagaaa ggcttttttg
    11221 ataccactcg atggccttta ctttgtcacc ttttcgttca tacatatctg ccatcagtaa
    11281 aacagcttca atattatccg gctgcatacc gagtacggtt tgtaaacggc ttattgcttt
    11341 gtcgtattgc ccggatatca tggttccttt aaccagcgtc atctgtgcat agatattggt
    11401 actgtctttt tccaccactt ccctgatttt agctattcct tccattggca tggctgaaat
    11461 attaccaaaa aggtagcagg cgccaaggcc tactttggca gaatcattat ccgggttaat
    11521 cttcaaagac ctttcaaaca aatctttagc ctgcagggct ttccactgtc ttcgttgctc
    11581 caaatcatca ttctgcaggt tctctaaaaa taagcgggct gcaaaggtga gggttttttc
    11641 cgaattttcc aatctcgctg cttccgcttc ataccaggca taaggttcaa agattttggc
    11701 tgaatctttc caaaaatgag ataattggtg gtagactctt aattgctgca cttttacatc
    11761 ccccctggta attgaatttt caagtgcatt taacctgaca acctgatctg tagtaagctg
    11821 ctttttagcc attagcagga tggtatcaat ggtaatacta acctttgtat ttccatgatc
    11881 atgcccatca tcctcgctat gttgttggtt atcagttagt gggattgttt ttttagaagg
    11941 agtagtcttt cctaaagtgt agattgcagc taccagcagt actgccacta ttattgtaat
    12001 ccattgaggt ttcttcacgg tcaaaaattt cagaatgcaa agttacccca aaccaagtga
    12061 gcaaattcag gttgtaaaag gaatctttgt attgattgct tattcggctt cttcgccagg
    12121 accagcatct ggttttggct ctttgagctt ttttacttct gccacgaatt ctttggcagg
    12181 tttgaaggcg ggaatataat gctcggggat atgaacagcg atattctttt tgatattacg
    12241 gccgatttta gcggcccttt tcttagtaat aaaactacca aagccgcgga tataaatgtt
    12301 ttcgccggca gcaagggtgt cttttacttc cttgaacata gtttcaagtg taacaagcac
    12361 atctaccttg ggtatgcctg ttttttcaga aatctgattt accaggtcgg cttttctcat
    12421 tgttgcaggg tttaagtgat tttacgaaga ccaatttatg acaaaataat atgatagtca
    12481 actaaatagg cataattttt ttcaaccgga tttgcaccag tcaatttgaa atgatggata
    12541 tttagtcttg aaataaaaat gaaggacctt tttttatgaa aaataacagc cgggaacagg
    12601 cccaaaagac atttgtaccg actcttttaa gatggaataa agaaaaaaat acccggcaaa
    12661 tgccctggaa aggggaaaaa gatccatata agatatggtt gagtgaaatt atcctgcaac
    12721 aaacccgggt agagcaaggg ctcaattatt ataacaattt cttaaaaacg ttccctgata
    12781 ttcataagct ggcaaaagca gctgatgaga aagtatttaa actatgggaa gggttgggtt
    12841 attattcaag atgcagaaac ctgctggtaa cagccaggta catttcaaaa gaattaaatg
    12901 gtaaatttcc tgataattat gaagatataa gaaaactgaa gggagtgggg ccctatactg
    12961 cagcagctat ttcatctttt gcatttaatc ttccatatgc tgtagtagat ggaaatgttt
    13021 tcagggtgct ggcaagaata tatggcatta ctatacccat agattcaacc gatgggaaaa
    13081 aatattttgc cactcttgcc gaaaacctgc tggataaaaa acaaccggga tattataatc
    13141 aggcaataat ggactttggg gctgtgattt gtaagccggt cacaccactt tgttctaact
    13201 gcgggttcaa aaagatttgc tcagctttca tcaataacaa aataaatgaa ctgccggtaa
    13261 aagagaaaaa aataagcatt aagaagcgct ggttttatta cctggtctta gaatacaaga
    13321 acgaaattgc tgtccggcaa cgcatagaaa aagatatctg gcaggattta tatgaatttc
    13381 ccctgattga agcggataaa gagatggata aaaaaaatat tttaaaacag gcagaaaaaa
    13441 acaaatgggt acacaaaggg aagtatgaat tgcttgctgt ttcgccgtta tttaaacaac
    13501 aactttcgca tcagttgatt gccggccagt ttattaaatt gaaattgaaa ggtaaccacg
    13561 atctggagag taattggatt tgggtgacaa aaagcaggat cggaaaattt gcctttccgc
    13621 ggtttattaa tcaatacctt aatgaaaaag gcgggcagca aagtttattc tgatgttaaa
    13681 ataatcaggg agggttcaat ttttattgtt tcaccttaaa aaaattttaa ctttaagcag
    13741 ctttgcagcc cttagatgat tctctaattc aaaaggataa aactatgaga ggcgtaaacc
    13801 gtgttatgct gataggcaat cttggtaaag acccggatgt gcagcacctg gaaggaaata
    13861 ttgccgtagc caaattcccc ctggcaacaa cggaaacttt taaagatcgc actggtaaac
    13921 tggtatcaca aacagagtgg cataccgtgg tgctttggcg gggactggca gaacttgccc
    13981 agaaatatct ccataaaagc agcctcgtgt atatagaagg aagactgcga acccgcagtt
    14041 gggatgataa ggatggcaat agaaaatttg ccactgaggt ggtgggtgac aacctcatca
    14101 tgctggataa gagaatggat ggaggtagtt caacaggaga tggcggagga atggaaggaa
    14161 tgggtggctc cgatatacca attggcgacc catctgaaaa cctgccattt tagtaatcaa
    14221 tttcaaatac aataactttg ttcgattaaa tcagggcagg tcctataacc tgcctttctg
    14281 acctgcctgt cggcagacag gttaaattgt aaacaaaatc ctttgctttg gaaagtcatt
    14341 cgatagttat tcctctcatt gataaccttt ctgttttcct gctggcagtt aatccgcagg
    14401 gaactacttt tttgggggtt actttgttag tcctcctgct actgacattt gctatctccg
    14461 gttctgaagt ggctttgttt tccctgaata aaaaggattt aaatatgctg aaaacaaagc
    14521 agcatgcatc tgccaaacga attgttacat tgctggaaga gcctaaagaa gtttatgcgt
    14581 cattgcttat agcagggacg tttgtgaaca tcagcattat agttctgtct aattttctgc
    14641 tctctgagct tatttcattt gacaaaatag gctctttgct ggaagtactc tttaaggtaa
    14701 ttatcattgc ttttgtactt gtattttttg gaaagatatt acctaaggtg tgggctacac
    14761 aaaataatct tcgttttgcg tatgattggt cttttgtagt ggaggccctt cacctgttac
    14821 tgcgccgcat cagtaaatgg attgtgaagc tggccgatgg cattggtaaa agagttggtg
    14881 cagacaagag tgatgtgtta agtatgcaag aactcgacga agcgatagat ataaaaagtg
    14941 atgaagaagc atctccggag gagaaaaata ttatgaaagg ggtggtgaag ttcgggaata
    15001 tctcagtaaa acaaataatg cgaagcaggc tgtatgtgaa tggcatcagt tacaattcca
    15061 ctttcagcga cctgataaag agggtggaag aactgcatta ttcaagattg ccggtttata
    15121 aagggagtct cgatgaagtt gtcggtatct tgaatacaaa agacctgata ccttacctga
    15181 atgaaggaga aaattttgac tggcatccat tgatgcgcca gccatttttt gtaccggaac
    15241 caaaactgat agaggactta ttaaaggagt ttcaacaaaa acggatacat tttgcggtgg
    15301 tggtggatga gtttggaggt accagcggta tcgtaacaat ggaagatatt ctggaagaag
    15361 tgataggcga tattaaggac gagtttgatg atgaagaaag cgacaactac aaactggatg
    15421 ataataatta catttttgaa ggtaaaatta tgattcatga tatgtgcagg atcatgaagt
    15481 tgccgatgaa tacatttgat aatgtaaaag gtgatagtga gtcgctggcg ggattattac
    15541 ttgaactggc cggtgaactt cccactgttg attgggtaat cccttgcggg gattttgagt
    15601 ttgctattat gcaagctgat aataatcgta ttcaattggt gaaagtgact attaaacaga
    15661 aatcgtaaat ccgtgataaa taaactaaaa aagtgttttt atataagcct taccgggtat
    15721 cttttttttc ttctttcctg caacagtgat tattctgccg gtaagaaaaa ggggtacttt
    15781 aagattgatt ttccggaaaa aaaataccag ttatttgagc agcccggcta tccgtattca
    15841 tttgaatatc ctgtgtatgc caacgtaatt aaggatacta cattttttga ggataaagcc
    15901 ggtgattggt ggattaatat tgaagttccc cgtttcaacg gccgtattca tgtgagttat
    15961 aaagcaatca acgaccaaaa taattttgac tctttagtac gggatggttt taaaatggcc
    16021 tataaacaac atgtggatgt tgctacgggt attaacgata gtgtgatccg gactccaaac
    16081 ggtgtggagg gaatttattt ttctcttggt ggcaataccg cgactgccaa ccagtttttc
    16141 ttaacagact cagttaaaca ttttttacgg ggtgctctat actttaatgc agcacccaat
    16201 gctgactcat taggtgttgt gaatgatttt ttaaaaaagg acctgcagca tcttatcaat
    16261 acgcttaaat ggaaataaaa aatcccccgc tcctggcagg ggatggaacg gattaatatt
    16321 tgtattactg atctttcctt tctgttttct tggtttcatt tttttgggca ggtaattctt
    16381 ccatttcttc tcttagcggt gcacggctga aaagtttggt acttaatttt ctctttcctt
    16441 ttttcacttt gtactctttc tttttttcat cagcatactt ttcactttta tcctcttttt
    16501 tagcttttat atttacaggt gtaacatttt tcttttcacc tgttttgtct tcttttcgga
    16561 tggcctgttt tgtaggttga tcattatctg ttttcagggt tgctacagca ggtttctctt
    16621 tttccactgt atacatgtcg ttgaactctt ttttattact tgcctggcta taatcaacaa
    16681 agccataaat actggcacct atcattaaag cggcacctgc atacaaaatt gatttcatgt
    16741 ttgattgttt taatgattgt taatgttatt tgcctggttt atgtcgcaaa tgatttaata
    16801 tattgtttgc tttgcctcca aatcacccat tctgcgaaaa acatattacc gattacactt
    16861 atccagagtg ataattcata attctccagg tggtcccagc caagtaaata gaatagtata
    16921 tggtataccc ggaaagtgac ggcagtcatt gccatagcat agctcctgat catacacact
    16981 ttatgggcga ttatatttct tttatgaatt gtgcttaatc catatagtgt ggttacaaac
    17041 caccagcctg ccataaacat aaataaagac ctttcccaaa aactgccttt cgcaaaaaat
    17101 gacatataca aaccagtcgg agctccgagg aacaggacaa cggatacata gatttttccg
    17161 gaaacccggt gtatagcttt tgatcttttc agtacatacc tggagaactg gattagagct
    17221 gtaccgatac atagagcccc agccgcaatg tgtatataaa agcaggcgtt gtaaaaacta
    17281 cctttaaaca gtatagacct ttcttcaata aaatcgaagt ctttactgaa agagaaataa
    17341 gggagagtat tataaatgat cagcaggctg aaaactgtta caggcaacca gaaaagcatt
    17401 tgtgcgaaaa ccttaaccga tttttgaaga gtgattatcc ttgtgttttg catacagctg
    17461 gcttgtgatt ttagggagat gctgctgtgt aaaactcttc atacatcctt gttgttagat
    17521 atttgttaaa ggaatttcat tggtggctga atctttatta atttcaggat ttacaacctt
    17581 gttatatgat ctattatcgc cgggtcttac tgtttttttt aagtatggca gcttccgttg
    17641 ttattcttgc gcaggaagca gcagctgtag attctctcaa aattgcactg gggaaagtta
    17701 ccacgataac cgaaaaggta aatatccttg atgagcttag ccgggtactg atgaatgtca
    17761 atccaaaaga agcggatgaa tatggcaata agctgatagc tgtggcggag gaatcaagag
    17821 acaggaaact gatgttcaaa gcctatttat caaatggtac acggtgttct tattttgcca
    17881 gccagaaagc ctattcacaa aaatctatag agttttacaa aaaggcattg gagctggcac
    17941 ggcaaaacag gatggaagaa gaagttggtg ccgcacaaat gcatttatcg acggtatacc
    18001 ggttgatacc tgataatgat aaagcacttt cctatgctac gcaggccttt tcattagtat
    18061 ctacgctccg gaatgatagt cttcgggcag aggcgcataa tgtatacgga caggcttacc
    18121 aggccaaaaa tgaaaatata ctggcattac gaaattatct tagtgctttg cgcattgctg
    18181 aagaattaaa gaatccttct ttgatcctga attgttacca atacctctct tctttttatt
    18241 cagaaattga agattatgat aaagccattg attatatgac gatggcctat aaaaagctgg
    18301 atgataccag ggagaagaat gcaccttatc aaaaggttat ttttaataat tatatcggta
    18361 acctgtatgc atacaaaaaa aactatgata tggctatcag ttactttgaa ctttccatac
    18421 ggatggctga ttcattaaag ttctcaaccc tcaaaatacc agcttatgtt agcctgctga
    18481 atcaatacct tcggattgat gaacctaaaa aagcattgga ttatttgaat tccccaccgg
    18541 gagataacct gaaaaaatac cttggcaatt ttggtatggc atcgatgatt gaccaggctt
    18601 atggggttgt ttatacagaa ttaggaaatt atgattcagc ccgtatttac ctggagaggg
    18661 caagaccgat gtttgagaat agcagtaatg aaaacaataa agtcaatttt tatacccagc
    18721 tggcatcttt ctatcgtaaa tcgggggaca aggaacatgc cattgagtat tacctgaaag
    18781 tgaaagagtt cagtgaaaaa aatggtttat tggaaaatgt aaaaagggca tcaagatatc
    18841 tggatacttt gtaccggcaa tccggagata ttgcaacagc tagtatttat aacgggatat
    18901 attaccagta taaggacagc attgaaacat taaaaagaga aaaggaactc acccaggtgg
    18961 aggctgcaga tgaacagcaa cgccaattaa gagctgtcaa agaaaaagaa gagaaggacc
    19021 ggcagcggaa caatatacaa tacctcgcta tcaccattgg tatcgtggca ttatttattg
    19081 gcttggtagt attgggaatg ttcaaagtat cggccaatac tattaaaatg atcggtttct
    19141 tcgcttttct gatgttcttt gaattcatat tcctgatctt taaaaagaat atttattcta
    19201 tcactaaagg agaacctgtt aaagatcttt tgttcatgat cgggctggca gcattattat
    19261 tgccgttaca ccactggctg gagcataaag taattcatta cctgacatcc cataacaggc
    19321 tgacttcagc cgggcatcat ataaaaagac gccttttcag gcgaacaaaa gaaggagatg
    19381 agtaacacag ggcttacctt tgccggaaaa ttactgagat ttgatagtta tagataacat
    19441 cctgattagt gaagacatag ttaataaaaa attcgtctgt gaccttgcaa aatgtaaagg
    19501 tggttgttgt gaagatggag ctgcgggtgc ccctttgagt aaagaagagc ttcagctggt
    19561 atcagactta tatgaaaaaa taaagcctta cctcactaaa gatgcggttg ctgaaataga
    19621 aaggaaaggg aaatatgtat atgatacgga atttgaatgg gtgacgccta cgctggatag
    19681 cgataatgaa atctgtgtgt atggtatccg gggacaagac gggataatca aatgtgcttt
    19741 tgagcaggca tacaacgatg gcgtgatttc ctggaaaaag ccaattagtt gtcacctgta
    19801 tcctataatt gccaaaaaag ggaagcatgg tgattacgaa agagtaaatt atgagccacg
    19861 agaacaaatg tgtagccccg cttgtgtgtt gggcgaaaaa ctgaaagtac cggtttacca
    19921 gtttctaaga gaaccaatag taagaaagta cggtgaagaa ttttatactg cattggacac
    19981 aattgcaaaa ggggagtggg aagaaattga ttttaaagag tagattgatc gataataatc
    20041 ttaataacca gttattgtga aagaaacagc tgccgatttt attgccagga gcactatcaa
    20101 agctgccgat aaagaacacc ggcgcaagat caattttaat attggcaaat acaatgcggt
    20161 cgtgccacag ggtaaattgc aatttacgga tgtacacctt gccagggaga gggctaagaa
    20221 tatcaagtgg aaggctatcg aaacactgga taaacaatta gaaacatttg aatcagctat
    20281 tacccggcgg ggagccaaag ttatctgggc agaagatgca caacaggctt tggatgctat
    20341 ccagaagatt tgtgaagaaa aaaattgtaa gacattggtg aagagtaaaa gcatggttac
    20401 ggaagaaatt cacctgaatg attttttaga gaagaacggc atcgaaagtg tagaaactga
    20461 tttgggagaa tatatccagc aattagacgg tgaacctccg tatcatatcg ttacaccggc
    20521 tatgcataaa agcaaggaag atgtggcgaa actctttgca gaaaaattgg gaaccgatcc
    20581 taaagctact cctgaacaat taacattaac ggccaggaga attctgcggg ataagtatgt
    20641 acaggctgaa gtaggactga ccggtgcaaa ttttattatt gctgatatag gcgctattgc
    20701 cgtaacagag aatgagggta atgcaaggtt gagttgttca atgccgaaaa cacatattgt
    20761 gatcgcagga attgaaaaag tgattccatc catgacggac cttgctttgt tctggccgct
    20821 gctttccaca ttcggtaccg gtcaaaagat aactgtatat aatactatca ttactggtcc
    20881 caggcaagct aatgaagccg atggtccaga ggatatgtat gttatattgc ttgataatgg
    20941 acgtacaaat attctggcca atgaaaagca aagagaaagt ttgtactgta tacgttgcgg
    21001 ggcatgctta aatgcctgcc cggtttacaa aaatatcggg gggcacactt atgcggctac
    21061 atacagtggc ccgattggtt ctgtcatcac cccccacctt aaaaatatgg atgaatggaa
    21121 gcaccttagc tacgcttcct cactttgtgg taactgtacg gaagtttgtg cagttaaaat
    21181 aaatctacat gagttattgt tagagaatcg ccatgaagct gtggaagaag gattcagctc
    21241 ttttacagaa aagatggcct ggaaaatgtg gaagcaggga atgctgcacc ggaaatggat
    21301 gaatatggcc ggtggaaata taaagaatag ggtagtgaat ggtttaataa aaggctggaa
    21361 ggaatatcgt ggtgatatgg agtttccgaa aaaatcattc aacgaattgt ggaaagaaaa
    21421 atacggtaac agatagttat cttcaatctg tgattctttt tgcaaatacg ataacgcacc
    21481 ggctaaaata tatctgcgat tttatcggaa cagaaattac cggtgaatcc attcgcatca
    21541 cagataatgt gctcagcttc gcagagcatg cgggtccgaa gatcaactac agtgatgaac
    21601 gtttaacgca ggaagaatta tggatacaac cttacctgct attatttgaa aaaaatatcc
    21661 acgagttaca gatagattgt tttgaaataa aagggtataa agcatttttc aaaacaaacg
    21721 gagatttccc attcgatatt tttgccgctt ctttttacct gcttagtcgg tatgaagagt
    21781 atctgccgca tcagaaagat atgtatgaaa gatatgccta tgaaaattcg ttggcatata
    21841 aagcgggttt tttaaataag ccgcttgtca atatctggtt acaggagttt aaagcgttat
    21901 tacaagccca attcccggca ttatcaaccc gtagctccac attcaatttt cttccaactt
    21961 atgatattga cgaagcatat tcttataagc ataaagactg gaagaggagt gccggtgccg
    22021 ccatcaaaga cttgctgaga agagattggc gcaggtttag attacgaaga aaagtattga
    22081 ataatgagct ggcggaccct tttgactcct atgaatggat agataatctt catcggccat
    22141 atcaactgaa accaagatat tttttcctgg tggcggagca aacgggaaaa tttgacagga
    22201 atattttgcc aaaagaaatt gccttgcaaa cattgatcag gcaacatgct gataagtatg
    22261 atatagggat tcatccttcc tggcaaagcg gtgacgattc tttactgatt caacaggaga
    22321 aagaaagggt tgaacatatt ataaaaaaga aaattgttgc ttcacgccag cattttatcc
    22381 ggtttacatt gccggaaacc tttcgtcatc tcattgaatc aggtattaaa aaagattttt
    22441 ctatggggta tggaagtatt aatggtttca gggcttctgt tgcgtcacct ttttactggt
    22501 acgacctggg aactgagcaa gcaacttact tgctattata tcctttctgt tatatggagg
    22561 ctaattcttt ctttgaacaa aagtattctt cacaacaggc attggcggaa atgcgttatt
    22621 tctataatga agtgaaaaag gtgaacggta catttatcac aatctggcat aatacatttt
    22681 taggaactga taagcgattt agcggatgga gagatatata tgcccgtttt gtaaatgaga
    22741 ttcatcacca gggcggaagg aaattacttt aaatcccttc tgtttccggt tgttcaggcg
    22801 gcggtacagc tttaaatgct ttatttacca ggtaaacacc cagcaatgtt acgagtccgc
    22861 ctactgtaat gaatatcgtg attctttcat tgaatatcag ccagccacat aacaccgcta
    22921 ctatcggatt gatatacgca taaatggaag cctgttcggt tggcaggttt tgcaatgcat
    22981 acaggtaagc aataaatgcc agtaaagaac caaaaataat gagataagca atggctgtcc
    23041 aggactccca tggaatttgt gaaatagaaa ctgcattacc ggtaaccgat gtaaaggaga
    23101 taagtgtaat accggatatc agcatttgca gaccaaggct gaaataagga ttgaagctgg
    23161 ccgcttgttg tttggtgtac agcgtggcaa aggcccatgt ccaggtggct atgagggata
    23221 atattatacc aaaacggaaa tccgggataa agaaatcttc caggtgttca taaaaaataa
    23281 tacacacacc actaaaacct aataataatc cgatgattgc tttctgcggc agtttttccc
    23341 tcgaagcaaa aagcccgata actaccagcc ataggggaaa tatggcaccc atgatagagc
    23401 ctaatcccgc agaaatatat tttacgcccc aggtactgag tccattactc ataatgaagt
    23461 tgagaaagct gagtactaat accggaatcc attctttacc ctttggtaaa ctggcgcctt
    23521 ttatcaggaa gaaagcaaca tatattaagc ctgcgataaa ctggcggatt ccggccaatt
    23581 gcaaagccgg catatgccgg actccttctt tggatgcaat ccaggtagtg ccccacaaca
    23641 cacaaaccaa tgctaacgca aaaagtgctt tagcttttgt ccccttcctg tgaatcgtaa
    23701 gtggattgaa taccgccagt ttctttaatg aatcagcaga tgattgaacg atcgttttat
    23761 gaaaatcaaa aaagttcatt aatgtttaaa atgccgcatg cctgtcatca ccatcgccag
    23821 gttatgcgtt ttacagtatt caatggaatc attgtccctg atggaaccac cgggttgaat
    23881 gaatgctgta ataccggctt catgccccag ccgtacagaa tcatcaaaag ggaaaaaagc
    23941 atcactggcc attacagctc catgcatatc aaaatcgaat tgctttgctt tttctaatgc
    24001 ctggcgaagg gaatcgatac ggctggtttg tccgcaacct ttacctatca gttgtttatt
    24061 ctttactagc gcaattgcat ttgattttaa atgcttgcaa ataaggtttg caaatatcag
    24121 atcctgtttc tcggtttctg tttcttttct ttttacagtt tcagtccagt tggtgaaatt
    24181 gccctcatca acgctttgaa ccaaaacacc gttcagtaca gatttaaact gtactttatt
    24241 acccggttgc gcttttaatt gcagcaggat cctgtttttc ttggatttca atacaatcaa
    24301 agcatcttca tcaaaagcag gagcaataag tacttcaaag aatatttcat tgatagcatc
    24361 agcggtagct ttatctatgg tgtcattaca aactaataca ccaccaaaag cactttcagg
    24421 gtcacctgcc agtgctgcat cccagcttcc ttttacagtt ggtctttgtg ccacaccgca
    24481 aacattggta tgtttgatta ttgcaaaagt agtatcgctg aattcatgaa tgagttgtac
    24541 tgctgcatct acatccacca ggttgttata ggaaagttcc tttccgttta gctggtcaaa
    24601 gactttattt aaatcaccat agaatacacc agactgatgg gggttttcac catagcgcat
    24661 cgttttttgc ggtttattaa aaggggtttc aatagcagat tggtgaaaat aattggaaat
    24721 agctgtatca tacgctgtgc aaacatcgaa tgctttaatg gcaaactgcc tgcgttgttc
    24781 tactgaggtt tctccctttt gaaaagcgag cagttttgaa aattcatcat agtcggcttt
    24841 tcgtgctatt actactacat ccctgaaatt cttggcagcg ccacgaatca tggaggggcc
    24901 gccaatatca attttttcaa tgatggcttt ttcatcggtt gttgaagcca ctgtttcttc
    24961 aaaagggtag aggtcaacaa tgacaaggtc aatctcagga atattatact gtttcatctc
    25021 ctgcaaatgt gttgtatcat cccttttccc cagaatgcca ccaaatacgg caggatgcaa
    25081 tgtctttacc cttccaccaa ggatggatgg atatgtagtc aaatcttcta cgggaactac
    25141 tttaatgccc atcttttcaa taaatgtttg tgtaccaccg gtggaataga tggttactcc
    25201 caattcagaa agttgttgta caatagtttc caggccgtct ttgtaaaaaa cggaaatcag
    25261 tgctgattga atcttcttgt tcataaaatt gaagggttga gaggcaggca attcgctaag
    25321 aagccgcaaa attaacttaa ttcaggttca tcacaaaagc accttttatg cttacatcat
    25381 gccagggaat gcgtaacgaa tcgcagtctt ttttccagta ttttgttttc caggccggga
    25441 ccgagccatc aaaaacaacc tgctttatat ccagtgagcc tgccagtttt gcaatatata
    25501 attttggatt ttttgaaatg accaccaggt cgataatggg tttatttacc tgcggtgtaa
    25561 atgaaatagt ttcatccagc agcagaatat acctcttttt atagctgata taatttttat
    25621 agcccaggaa gtcattcagt gtatctgcac cttttatccg gtataagacc cgggagggct
    25681 tcaggtgaaa attccgggca aaatcgttgg ccaataatcc actgtcgccg ataaacaggt
    25741 aatttctccc atcaataaaa tcaattgctt ttttttgagg aacattatac acaataattt
    25801 tctgttgctt ttcggcagca ataaaggata cggttcgttc tgctgtgaat aacagtaaag
    25861 aaactatccc gattttcaac ccgtttgttg atctttccat caaccagtaa ctgattccgg
    25921 ctgtaaagaa aagtaaaagt attgcctgtg tgatggagag ttgcaagcca tcccaaagtg
    25981 aaaatggaat taattcaatt ctttccacgt aggtgttcat gagccagatt aatgaggtga
    26041 gtattttacc ggccaacgca gcaactgcag gtataagcga tacgatgcat aaaagaattt
    26101 cgccaaacag tataatgctg gataagggaa cagccaggaa attcgtgagt ataaatgaag
    26161 ttgggaattg gtgaaagtga taaataccca gagggattgt cagtatttgt gcagccaggg
    26221 taacagcatt tagtttccag acaaaatcaa ggatcttatt ttttatatta aaccagttat
    26281 aaatcggacg catgaaaatg ataatactca gtacggctgc ataggataat tgaaagccga
    26341 catcccaaag ccagaaagga ttgtaacaaa gtaaaataaa tgcggagacc gccattgtat
    26401 tataaattgt agttttcctg gtgaaggatt ctcccagtac aatacagcta aacattaatg
    26461 ccgatctgag tacagatggt tgagcccctg ccagtaaact gaacgaccat aatcctgcaa
    26521 ttattaatac agggctgagc caccttgttt tcctgtttcg ccgtagtggc tttaacagtt
    26581 gtaccagtaa ccagtaaatc aaaccaaggt gcagcccgga gatggcaata atatgtacga
    26641 ctcctgtatt ggtatatgat tgcaccagcg actggtcaag atcatcttta taaccaatga
    26701 gtaatgcttc tgcaaggccg agctcttttt ccccgctaat attctggcgt aaaatagtaa
    26761 gtaccttttc cctggacgta aatattagtt tcttaagcca gttttctttt ttacccggta
    26821 ttatttcaaa ttcccccggt tttagaaaaa cctggtgagt gataccctgg aaaagagaat
    26881 agcgtttgta atcaaatcca cccgggttgc cggaattttt tatttcctgc aatgattttt
    26941 tgaaaattac ctgcgagcca taggaaaact gttgtccggc agaatccttt ttaaaataaa
    27001 ttattatttt cccctgagca ggatttattt ttccttcccg gatcaggaaa gagacgctgg
    27061 cattggcttt atttgatttt gttttttcaa caggtggttc atccagtgtt gcaataatgg
    27121 catctttatc tttatatact gctccaagcc attgaccgtt gttgcgaata tcattttgaa
    27181 taaccagtaa agcaccaaag gaaaggaaag tggttccagt tgatatcccg ctaaaggtgg
    27241 agagatgaaa acgagtaagg accggtataa aaaagaaact acaaagcccg gttattccaa
    27301 cggctaaaat gattaaccat gtatttgcat ataactgtaa atgccactga acaataattc
    27361 cgagcatcag tggtatcagc agcttaagaa aaggagtttt cttccagaac cggttgccat
    27421 aagaagccat atggaaaagt aagaacttga ccttgccccg gcaatactac tttccgggta
    27481 tatcaggaca gcattttgtt gaggtaggca acctgtccaa gatggtaaat agaatgttct
    27541 atcatcccgt ttagcagaaa acggtaattg tatttgcgat agtccactat ctccagcaga
    27601 aaatcatcat cctttttatc cagtaaggca attatctttt tatttatggc cttaaactct
    27661 gccagtgctt tcttccagct atgcagtttc gggttaagct cccgccagtc cattttttca
    27721 aatgctgcca tatccatttt attattcttc tccaatcttt tgagtgtaaa atctgcccag
    27781 gttatcatgt gataaagcag ttccagcata gagtgttcgg tgcagtttgg tttgatggaa
    27841 acctttttcg catccacttc ttccagtata acaaacactg cccggccaaa ccagggctcg
    27901 ccactgttca cattctctat ccggcggatg atgctctggg tctctttatt catcttttta
    27961 taataaggta gttattctga aataaggttg atgctgagcc ctgttttcga ttgaaggcca
    28021 cttgcatttt gattattcgt tagtatggta ataaaaaaag aaaactgtgt tcctgcaact
    28081 aaatatattt cccatttttt tctgatagtg gagctatagg tatgtccaga accaaaaatt
    28141 cccatataat ccagcccgcc tgtatagaaa ttttcagtaa aaccactggc atttaaatcc
    28201 aaaatacata gtacggaagc attttcaaca gttttgtcgg tataaggagc aaaatcggca
    28261 tttatactat aaaggccgct tttatttaca ttgaatgtgc cgtttgaatt attgtagctt
    28321 attgcagaac tgttggaata gacagtattg tatagaattg tttgagaaaa aagacctgat
    28381 gtccctcttt ggttgtcagc gagagtatag tagaagcggt cattattgct ataggcaata
    28441 ttttgccagt taggcgctgc gccatttcct ttgcttacaa gtacctgacc attattgccg
    28501 gcagaattgt tcagcaacaa tttattctca atgtttacat ccccatttat atccagagtt
    28561 gtttgcgggt tattggtgcc gatacctaca ttcccatttc cttttatccg cattgtttcg
    28621 atcaatgagc tgctgttgcc gtttccgaat acaaaatcat cagtattcgc aggagtgtaa
    28681 aactgcattt gaaaggcctg aataccgatg ccataatgat tggggttagt tccccacaag
    28741 gcaattttat tcccaagtgt attggaaaaa tgcagcggcg atgtagggct cggttgcccg
    28801 atgccgatat tacctgaagt gctgatcgaa agtttagccg aattattggt tactaatgat
    28861 aaagggttat tagtggtggt accgatataa ggaccagttc ctgaaggttc attgctaatc
    28921 cttaaaatag atcctgcatt ttcttccagc acaatttgtg taccgctacc cgtgcctttt
    28981 acatggagct ttgaaaccgg tgttgtggtg ccgatcccaa cattttgtgc taatacatta
    29041 acgtaagcaa taattactaa taaaaacagt atggcttttt tcataaaaaa aatttctact
    29101 gtaaattaat aaaaagaggc ttattagcaa tcgccaaaat gggtgatcag ttcaggcaat
    29161 aaaagaagac gatatgcaat tccggcaact catctttaca accaaagaac aggaaatgca
    29221 tgaaatagta aaaggattga aatgaataag aaaatggatc gtgtaatacc agctgtgtct
    29281 tattccataa aactatttac tcaaatacat gctcctgtct ttatacaatt ggcggaagta
    29341 aggatctctc agatctttaa tgaaacggat tgcttcccct gtacttttca tttccggtcc
    29401 tagttcttta ttaacaccag gaaatttatt gaaactgaat accggttctt ttatcgcaaa
    29461 cccctttaat tttttctcaa aagtgaagtc cttcagctta tgcgtaccaa tcattatttt
    29521 ggtggcaata ttcaggtaag gtatctggta tgctttggca ataaagggtg ttgttctgga
    29581 ggctctcggg ttggcctcaa ttacatagac tttaccatct ttaatggcaa actggatatt
    29641 gatcagacct ttgatgtgca aagcccttgc aattttttca gcatactctt ccatggtatg
    29701 aacgatcaat ggtgtcaggt tgaatgcagg caacacagca ttactatcgc cgctatggat
    29761 accggccggc tcaatatgtt ccattactcc catgacatga aaatcattcc catcaaaaat
    29821 tccatctatc tcagcttcct ggcagcggtc aagaaaatgg tcaatcaata tcttatttcc
    29881 cggaaggtgt ttcagtaaac tcactaccgc tttttccact tcttcgtcat taagcacaat
    29941 ccgcattctt tgtccgccca acacatacga cggacgaacc agcaccggat agcctacttt
    30001 attggcaacg gcaatagctt catccacatc ttctgcagtg ccatattccg gatagggaat
    30061 gtctaattcc ttcagccggt cactaaaacg gcccctgtct tcagctatat ccatactgtc
    30121 gtaggaggta ccaatgatct ttattccttt ctgatgaagc ttttctgcta atttcagcgc
    30181 cgtttggcca cccagttgta caatcacacc ttctggttgt tcatgctcaa tgatctccca
    30241 gagatgttcc caataaaccg gttcgaagta caatttatct gccatgtcaa aatctgtact
    30301 cacggtttcc gggttgcagt taaccataat tgattcatag ccgcattctt ttatggcgag
    30361 taatccatgt acgcaacagt aatcgaattc aataccttgt ccgatccggt taggtccgga
    30421 gcccagtaca atgatttttt tcttatcaga aactttactt tcattgctgt tcccgctttc
    30481 gaaggtggaa taaaagtatg gagtcctggc ttcaaactcc gcagaacagg tatccaccat
    30541 tttgaagacc cttgttattc ccgcttcttt tctttttttg taaacgtcat cttcatcacc
    30601 atgctcggta atgcggctta tctgttcatc gctgaagcca tttacttttg cctctctcaa
    30661 taattcaatc ggtaaatctg ccaggtcgta ttttgcaatt tctttttcaa ggttacagat
    30721 cttctggatc tcatacagaa accagcggtc aataccgtta gttgctttcg atattgtacc
    30781 aacagagata ccggccatca gcgcatcttt aatacgaaag atgcggtccc atttaggtgt
    30841 tttaatatac tcaagcagtt catctgcatg catcaggctt ttgccataat agccaagacc
    30901 aaccgcatta ttttccaggc tttgacaggc tttctgaatt gcttctgtaa agcttctgcc
    30961 aattgccatc acttccccca cacttttcat ctgcaatccc agtgtatcat tagctccttt
    31021 gaatttgtcg aaattccagc ggggtatctt tacaatcaca taatccaacg ctggctcaaa
    31081 gtaagcagat gttgttttgg taatttgatt ttccaattcg tcaaggttat aaccaatagc
    31141 cagcttggca gcaatttttg caattggata gccagttgcc ttacttgcca acgcagagga
    31201 acggcttacc ctggggttga tctcaatcgc aattatttct tctgtatcag ggttcaatgc
    31261 aaactgaaca ttacaaccac ctgcaaaatt tcccaggtcc ctcatcatca tgagggctgt
    31321 atttctcatc agctgcatac cagtatcact caacgtcatg gcaggtgcca ccgtgataga
    31381 atcaccggta tgaacaccca tcgggtcaaa attctcaacg gtgcaaacga tacaaacatt
    31441 atctgctgaa tctcttaata attccaattc aaattctttc cagcccaata ctgctttttc
    31501 aaccagtact tcatgtatcg gtgatgcctg cagaccccgg ttcagggctt catccaggtc
    31561 atctttatca tgtacaaatc cgccaccagt accgccaaga gtgaatgatg ggcgtatgac
    31621 caatgggaat cctatctgtt gtgcaaattc ttttccttct aaaaaggagt tagctgtttt
    31681 agcagtagct actggtactc ccaaagcaat catccactga cgaaattttt cccggtcctc
    31741 tgctttatcg atggctttaa tgtctacacc gatcaaccgc acattatatt gtttccagag
    31801 gccaagatct tctgcttctt tcgccaggtt cagggccgtt tgtcctccca tggtcggcaa
    31861 cacggcgtct atttcatttt cctgcagaat ctgctcgata ctttcaacgg tgagtggtaa
    31921 caaataaacc ctgtctgcca tcatggggtc tgtcataata gtggccgggt tgctattaat
    31981 aagaataacc tttattcctt cttcccgcaa actcctggcg gcttgcgttc cggagtagtc
    32041 aaattcacag gcttgtccga taatgatggg gccggagccg ataatgagaa ccgatttgat
    32101 cgaattgtct tttggcattt tttgttttta gttgttgtag gcagccttcg ttatgaaaaa
    32161 acaaattgcg caaaagtaag ggaatagaag tgttttgagg agataagggg gaggaaaatt
    32221 tccagctatt tttcaatttg attgaaagtg cggcgttttt ggaatttttc ggtacgaaag
    32281 ggaatactgc tcatcataaa tatttttctt tccactcatt tcgatgacga gctttttcag
    32341 gtcccccggc tggtttatta tttttcgggt ggtaaagctt tctgttatgg tttttacccg
    32401 taaagtatca ttactttttt cgaaagcgga cagataaaag acgttttttt cagttgtttt
    32461 ggtgtttttt tttccgattg gttcagtaag ggttattgtt cctgttacat tcatgaaccg
    32521 ctggtcaccg atccatgagg tataggctgt gaagtatgac gtatctggta agatcatatc
    32581 tccctttacc gctttaccat aacgtataat tgaataaaca gaatctgatt gtctggatat
    32641 ttctaatgct tcaatgccga aatagtcact gccatctttt acaattccat accaataccc
    32701 aatcagggta ctgtcaattg gttcagccgg atttttttcc agcggaacca ttg
//
LOCUS       DAORTI010000026        31626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_968_length_31626_cov_6.578506, whole genome shotgun sequence.
ACCESSION   DAORTI010000026 DAORTI010000000
VERSION     DAORTI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 31626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31626
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_31626_cov_6.578506"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..625
                     /gene="pyrR"
                     /locus_tag="PLO70_05455"
     CDS             116..625
                     /gene="pyrR"
                     /locus_tag="PLO70_05455"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQZ73938.1"
                     /translation="MQVLLSSQQLADTIKVLSQQLSKSYPSLENIVFVGIQQGGVVIA
                     NDIVALLQQQHSSEKVQFGQLDITFYRDDIRKEILAPDTMNIPFAIENKIVVLIDDVL
                     FTGRTIKAALDALLDYGRPAKVELCVLIDRKEHRQFPIQADYVGATVTTKKTDKVKLV
                     NREVVLIPA"
     gene            726..1649
                     /locus_tag="PLO70_05460"
     CDS             726..1649
                     /locus_tag="PLO70_05460"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQZ73939.1"
                     /translation="MQLSTRHLLGIKNLTKEDISLILSTAEQFKEVLQRPVKKVPSLR
                     DITIVNLFYENSTRTRFSFELAEKRLSADTINFAASSSSAAKGETLLDTVNNILSMKV
                     DMVVMRHSASGAPHFLSQHIPAAIINAGDGINEHPTQALLDAFSIKEKTGSLEGRKVA
                     IIGDIMHSRVAQSNIYLLNKMGAKVTVCGPPTLIPKYISEALGVNVSYNLRETLEWCD
                     VANVLRIQLERQNQVLFSSLREYSIAYGINRQLLDSLQKEIVIMHPGPINRGVELDSD
                     VADSKQSIILQQVENGVAVRMAVLYLLANRS"
     gene            1726..2193
                     /gene="coaD"
                     /locus_tag="PLO70_05465"
     CDS             1726..2193
                     /gene="coaD"
                     /locus_tag="PLO70_05465"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQZ73940.1"
                     /translation="MRICLFPGTFDPLTLGHVDIINRALPLFDKIVVGIGLNASKAPM
                     FSPEQRLEWIREIYKDEERVEGAVYEGLTIDYCKKIKAHFILRGIRYVSDFEYEKTIA
                     DANRTLDKSIETIFLTGEPKYTSVASTIVRDIIRNGGNAAPFLPDVVSKSLVK"
     gene            2198..2641
                     /locus_tag="PLO70_05470"
     CDS             2198..2641
                     /locus_tag="PLO70_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HQZ73941.1"
                     /translation="MSIWFKKDLTIADISLLGKQTMSDYIGIEWKELGEDFLKATMPV
                     DHRTKQPYGLLHGGASCVLAETIGSLASAMVVDHSRYFCVGLEINANHIRSARDGFVT
                     GVARPLHIGRNTHVWDIKIYDRSEKLVCVSRLTVAVIARKDTGSV"
     gene            complement(2633..3259)
                     /locus_tag="PLO70_05475"
     CDS             complement(2633..3259)
                     /locus_tag="PLO70_05475"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQZ73942.1"
                     /translation="MYIKIYFNNKPLFLCDNVDETIQPYIHHDDAVFIDELNTHTIKS
                     MIHEMEQEKVHAGVFFHPDLEELKKAFYKKFTIIIAAGGLVQNEKKEILLIFRRGKWD
                     LPKGKLDAGENLETCAVREVTEETGLQHIKLISPLTITYHTYHEGSRFILKESHWFKM
                     KVKGEQELIPQMEEDIHEIKWVKAGELVDYIKNSFPSVVDVLEMISSH"
     gene            3288..3923
                     /gene="pyrE"
                     /locus_tag="PLO70_05480"
     CDS             3288..3923
                     /gene="pyrE"
                     /locus_tag="PLO70_05480"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQZ73943.1"
                     /translation="MTDAKVVAEKLLQVNAVKLNPEQPFTWASGWKSPIYCDNRKVLS
                     FPFIRDYIKSEMCNVIFDRFPEAELLAGVATAGIAWGAMAADQLKLPYVYVRPKPKEH
                     GLGNQIEGFYEKGQKTVVIEDLVSTGKSSLQVVDVLKEQGLDVIGMVSIFTYGFPAAT
                     EAFSNASLTYHSLTDYPTLIELAVEKGVVSAGQQEILLKWRDDPAGWKGIL"
     gene            3947..4336
                     /locus_tag="PLO70_05485"
     CDS             3947..4336
                     /locus_tag="PLO70_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQZ73944.1"
                     /translation="MKKYVIIAFCLIGIAIAGNAQTKPVVLATIKTPNALCPICKTKI
                     ETYLKRYDGVMEINVNYRKGETRVKYITDRTDIEQIKTAIANTGYDADDVPATEEAYN
                     RLPKSCKKFEDGGGHPKPKAPPAQLQQ"
     gene            complement(4370..4819)
                     /locus_tag="PLO70_05490"
     CDS             complement(4370..4819)
                     /locus_tag="PLO70_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73945.1"
                     /translation="MAFLGTFLITILLMSCSSFREPDFKGIENVKTTAISLSHTSLAI
                     DLHYFNPNNTQIKLKNAEGDAWIDGTPLGRFFMDTLILIPAKADFRLPVKLELEMKSV
                     LKNSLAVFLKNEVMLKIEGQARIGKAGFYVNYPVRYEGKQNISGLLK"
     gene            5436..10640
                     /locus_tag="PLO70_05495"
     CDS             5436..10640
                     /locus_tag="PLO70_05495"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73946.1"
                     /translation="MRKLHLLSTLLLAIFTGGISYAQDFSNKGKDFWVGYGYHQIMSG
                     TNLQDMVLYFAADQNTNVTVTIPGIGYAQNYFVAANTVLTSSPIPKTGLQDARLLTES
                     TIPEKKGIHITSDKPIVAYAHIYNQSVSGATILFPTTTLGKEYFSVNYTNISNSPNSN
                     CWFYVVAADTGTTTVEITPSAATINRPAGIPFLVTLQQGEIYNIMGQVSGNTGVDLTG
                     SKIQSINTGAGCKRIGVFSGSGRISITCNGISSSSDSYMVQAFPKSAWGKKYLTASAS
                     GGMDNNIYRVCVTDPSTIVTVNGAPIAVPLQNGFYYELPITPSPMKIEANNPILVAQY
                     FTSTQGNTGCGNAGPGDPEVIYLSPVEQNINKVLWNATPNFAIGSHFFNVIVPNTGTG
                     LSSFRLDGILVSPSLFTVHPQDPAYSYLKQSVGSGQHTIQSDSGFNAIAYGFGSFESY
                     GYNAGTNIKDIYQYISVQNPQATVNFPATCRGTPFYFSMTFPYQPTSIQWQFGPALNA
                     MGIADVNIPAPVPTSTTVVNGKTLYNYQLPTPYVITVAGTYPIRVVATNPTPDGCGGT
                     QEIDFDLQAFDPPLADFNFTTNGCVSSPVSFTDNSNTNGRPVISRHWNFGDANTSALN
                     NPSHTYAAPGAYTVKYSLITDIGCIADTASRVVTINDPPVALFTAAAPYCEGRSVTFT
                     DQSTSPGGATISQWTWDFGDGSPVVVRNTSTNETHTYASAGSYTATLRVVTSSGCQST
                     LFSFPVTIYPNPVADFNLPVACLPVGAAQFNDLSTVGGGNTITSWAWNFGDAGTSTVQ
                     NPSHNYSAPGPFNVSLTVTSNNGCTDTKNQVLNTVYAEPQAAFNSLPEVCIGSLIGFS
                     DASTAPGSTVTGWSWNFGDGSPISTIQNPTHIFSTAGTYTVTLNVTSAAGCQTMNNSA
                     TRTVVVKPLPTGSITGAIAVCLNAPSPNVTFTGGTGTAPFTFTYTINGGPVQTVITTS
                     GNSVDVPVPTNTAGTFIYTLTSVKEGSSASCVQSQSGSVTVTVNALPTATITGITTVC
                     LNAASPAITFTGASGSAPYTFTYNINGGPNQTVTTTTGNSATVTVPTTTAGTFTYNLI
                     SVQEGSANFCNQAQIGSAVVIVNPLPTASISGDNEVCLNSASPNITFTGGAGSAPYTF
                     TYNINGGPNQTVTTTTGNTVTVPVPTTVAGTFTYNLISVQEGSANLCNQAQIGSAVVI
                     VNPLPTGNFTFTAPSCETRTITFTDASAANAGTINNWQWNFGDPGSGASNTSTLQNPS
                     HTFVAAGTYTVTLTVTTDKGCVSNVYSNPVTINARPKAGFISPEVCLTDPLATFTDTS
                     SVVGGTIVAWEWNFGDPNANAGNPNTSVLQNPTHRYTVVGPYTAQLIVTSNNGCKDTT
                     QQTFIVNGSIPVAGFTVLNPSTLCSNQSVSITDASTVDFGNLVKVEVFWDYLNNPTIK
                     TTDDNPIPGAVYTHSYPEFGTPFTRTFQVRYVAYSGINCVNTTTQTITVLATPTLQFA
                     PIPFACTDKPSFQITQAQLLNGLPGAGVFSGTGVTPTGLFSPSIGAGQYNIRYTYTGT
                     NGCSNYKEQTVDIYTAPAINAGPDKFVLEGGQVTLTPALNVGVPLTYMWTPPTGLDNV
                     TSSFPVASPTDDITYTLTVTTPQGCTASDNVFVKVLKAPIIPNIFSPNGDGVHDRWDI
                     PFLESYPGCTIDVVNRYGQLIFRSVGYTTAWDGRVNGKDVPVGTYYYVIDPKNGRAKV
                     TGYVDIIR"
     gene            10645..11694
                     /locus_tag="PLO70_05500"
     CDS             10645..11694
                     /locus_tag="PLO70_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQZ73947.1"
                     /translation="MKRLLLTVVIVFCAGLVADAQQKPHYTQYVVNPFIINPAIAGID
                     NYVDLKMAVRDQWVGINGAPKTTYLSIHGPLGKNDYRTSSTSFQVPGENPRGKAYWEN
                     YTAAEPHHGIGLSVINDRTGSFNFFSASATYAYHLGLSPTTNLSGGLSAGITKVSIDA
                     SKHDFSGTGDPSDPATGSGFSSELTRIRPQMAAGLWLYSRDYFIGFAAQQILPQTLNF
                     VDDNASILTKGRLVPHLFLTAGYRFLLSDDINAIPSVMVKYIKGSSFNEFQPEVNLKM
                     QYRDQLWVGGSYRYQDGFAGMLGLNVSNTFNVSYAYDKSSNNNILRDFNSGTHEVVFG
                     FMIGNKYSEACPRCY"
     gene            11874..17009
                     /locus_tag="PLO70_05505"
     CDS             11874..17009
                     /locus_tag="PLO70_05505"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73948.1"
                     /translation="MLSAQDYSNKGKDFWIIYTGHYDGTNSRMALYITSDLNASGTLS
                     VGGSTINFTVTANQVTTLRLTNATIPSNSLAYNGQTIGIGTNKGIHIVTDTPVVVYSH
                     ILNAARSGSTLVLPTKVLGREYYVSSYASSGNLTPRSSEFAVVATLDNTTVEITPKQA
                     DANNTYPANIPFQVTLNKGDVFQFQSANGGDLTGSFIKSIAAAGHPCIPIAVFSGSTW
                     TAMGCAGAGSGDNLYQQLFPLVSWGQYYITAPFKLRSYDIFRIMVKDPTTVVQVNGVT
                     LNPAILINNSFYEINTIGDNTPRIITSDKPVCVVQYMITQSCDGVNSDPEMIILNPIE
                     QTLSDITVLSARNDLTPPNTNITRHFLNIIIKTAGLGSLRIDGAPYTSTPVAIPSTAY
                     SYLQEEVTASTNINPSHRVYADSGFIAIAYGYGNVESYGYNAGTNVRDLYQFVSVQNQ
                     YATVPFPAACKSSPFYFSMVFPYQPTQIAWDFNGLFSNVTINNPVYDSTWFVNGRQLY
                     RYRLTAPYTINTAGTYPIRVIAQNPTPDGCGGEQEINYDLQVFNPPVADFSYTPACFP
                     NPIQFSDNSNTGGRPVISRYWDFGDATYAAVNNPSHIYAATGTYNVRYALITDVGCLS
                     DTTMHPVTLSPLPTASISGTIEVCQNAPPPVITFTGAVGTAPYTFTYTINGGPNQFVT
                     STGNTATVPVPTATAGTYIYAIANVQDASPAVCNQAQTGSAIVTVNPLPTASISGSTA
                     VCKDAPSPNITFTGATGTAPYTFIYTINGGANQSVITTSGNSVTVPAPTNAVGTFTYN
                     LVSVSDGTATICSQLQSGTATIIVNPLPTASVAGTTEVCQNAPSPIVTFTGSSGIAPY
                     TFVYTINGGPNQVVISTANIATVQVPTAVAGTFIYNLVSVTDASTTICSQLQNGIATI
                     TVYPLPSANFNFSAPLCSEFVVDFTDISNPNVGNLVSWLWNFDDPGSGAANTSALQNP
                     VHTFNAPGIYNVSLIVTNDKGCVSNSFIRQVEIKPKPLSGFVLPDVCLNDTYAQFNDT
                     SSVASPGLIAAWQWNFGDPGSGINNTSVLQNPQHSYTAVGTYNVELIVTTSDACKDTV
                     EQTLSVNGSFPVADFVVNNPTTLCANDSVAIIEASTVFPGNITKIEIYWDNNGQPAAF
                     DSDNNPFPGKTYKHLYTNFQTPLTRTFTIRYRAYSGGVCVNDIFKDITVNAAPSVQFS
                     AINDVCLDAVPFQITQASETGGVPGSGVFSGPGVSPAGIFNPSVAGPGVHTIKYLFTS
                     ATGGCSDSLTQSIKVFEPAVAAFSVSAPLCERKAVTFTQSSTTPEGILTTWIWDFGDG
                     SPLLTVSNGNPVTHIYTTWGAYDVKLKVVTSNGCVSLEKLLRINVNPQPKPDFSIPVS
                     LCLPAANATFTNLSTIADGTQNLLSYYWDFGDPASGTVNNSTATNPSHMYITTGPYTV
                     NLQVTSGAGCVNDTTILLNTIHDQPIASFITDKPEACLGQSIRFTDNTDPMGGITNQW
                     NWMMDDGNTRNTSTFNYTYSSAGTYNVSLYSFNSFGCRSTTYTTPVVIHPYPTVDAGP
                     DRLVLEGGQVVLQPVVTGNDMIYNWTPNLYFTGSNTVLNPTINGVDDITYTLTVTARG
                     NCAKSDQVFIKVLKYPIIPNIFSPNGDGVHDRWELPYLESYPGCTIDVVNRYGQLVFR
                     SVGYATPWDGKVNGKEVPVGTYYYVIDPKNGRKKITGYVDIIR"
     gene            complement(17050..18765)
                     /locus_tag="PLO70_05510"
     CDS             complement(17050..18765)
                     /locus_tag="PLO70_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQZ73949.1"
                     /translation="MTGIQLHLLQSLGWAVLNSLWQMALLWVIYMIVIAISKNLKNSS
                     KSILASGLLFAGFLWFVYTFIIVYNKIITGEAVGESVTISSGTYSWLLQSLPFISVAY
                     LLLLTLPVLRFIRNYRYVQVIRKYGLTKINVDWKIFVRNVAARMGIKKPVQIWVSEFV
                     SSPVTIGFLKPVILVPLAAISHLTPHQLEAVLLHELAHIRRYDYLVNLLINFIQTILY
                     FNPFVKAFVKIVEREREKSCDEMVLQFQYDSHQYASALLLLEKTNYQQKALIISAGGN
                     KNDLLHRIETIIGIQKKQTLSANKIAGLLSAIICILALNLLLLLPGKESNRNKTTAYS
                     EIFSPFSFITEGAGEQNDEAEAGNKKSALTIFDSKNLAATTDVQSPATPIEPPPNTPL
                     ISEQNLQDISSLAYKLAGFEEVNIPVLKKYQEQQVKDALAASKKVLESSQWKLVEKSI
                     ADAFSEREKEEIKSVYEKELDKFDWNKWENKLKLAYDNIDWDKVNNQLSNAINQIRVD
                     SLQRVYNDVAVKLNAIEKELSLNKVKSIPDTDISLKTVEEKKTKVQQTLNTLKAIRNK
                     KIVHL"
     gene            complement(18768..19157)
                     /locus_tag="PLO70_05515"
     CDS             complement(18768..19157)
                     /locus_tag="PLO70_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQZ73950.1"
                     /translation="MSAPKLIKPTESELEILQILWTKGTATVRDVHEELAQTKDVGYT
                     TTLKLMQIMNEKGIVKRDDSMRTHVYQAAVNKEKTQKHLLGKIIDNLFGGSPTQLVIQ
                     ALGDESHKASPEELEKIQALLDSLKKQ"
     gene            complement(19469..21715)
                     /locus_tag="PLO70_05520"
     CDS             complement(19469..21715)
                     /locus_tag="PLO70_05520"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQZ73951.1"
                     /translation="MDKLIYLVPVMGLIGLLYTLVKFNWVSKQDAGNDRMKEISTYIA
                     EGAMAFLKAEWKILGYFVVIVGILLAVMASSNPHSHWLIAVAFVAGAVFSATAGYIGM
                     KAATKANVRTAHAARTSLSKALNVSFTGGAVMGMGVSGLAVLGLGGLYIILKQVFAPD
                     SAVNSDEMIRTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATIVLGQQTTVVFGDDLLGGFSPI
                     ILPMLIAGVGIIFSIIGTLFVRVSDSAGISTDVVQKALNMGNWGSIVLTAGASAGLVY
                     FILPEAMELRGVMFTKWGVLGAIGVGLLVGALMSIITEYYTAMGKGPVKSIIKQSSTG
                     HATNVIGGLAVGMESTFLPILVLAGGIWGSYQFAGLYGVAIAAAGMMATTAMQLAIDA
                     FGPIADNAGGIAEMSELPKEVREKTDVLDAVGNTTAATGKGFAIASAALTALALFAAF
                     VGVAMPDNQHIDIYKADVLASLFVGGMIPFIFSSLAIRAVGQAAMAMVEEVRRQFRTI
                     PGIMEGTGKPEYDKCVAISTQASIKKMMLPGAITIISPLIIGFLLGPEALGGFLAGAT
                     VSGVLMGMFQNNAGGAWDNAKKSFEKGVEINGEMYYKKSEPHKASVTGDTVGDPFKDT
                     SGPSMNILIKLMSIVSLVIAPTLAQINAKKVEVPVVPEAPKPMGKEFRIKSEPMAAIN
                     KANTVQYQ"
     gene            complement(21881..>22782)
                     /locus_tag="PLO70_05525"
     CDS             complement(21881..>22782)
                     /locus_tag="PLO70_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQZ73952.1"
                     /translation="LDIGLHQDFYKSLNSRFELLDDTIIHSIHKPRNRFAHKGHYGHA
                     LLVAGSYGKIGAAVLSAKACLRSGAGLLTCHIPSCGYSILQTAVPEAMVMTDFNSSFN
                     TKLEDDLMKYEGIGVGPGIGTASETKMMLREIFDTYRNPVVLDADALNIIASQKDLLN
                     LVPVGSILTPHPKEFERLFGEAANDFDRVRLALEKAKELSSIIILKGHHTFIATPDGR
                     GFFNSTGNPGMATAGSGDVLTGILTSLLAQGYSSSETAVLGVYLHGLAGDLAAKELSM
                     EALLAGDIINFLGNAFRFLQQNN"
     assembly_gap    22783..22882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22883..23242)
                     /locus_tag="PLO70_05530"
     CDS             complement(<22883..23242)
                     /locus_tag="PLO70_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQZ73953.1"
                     /translation="MKILSAEEIRLWDQYTIQNEPISSIDLMERAAIACVEWLLKKYP
                     DAPSFGIFCGKGNNGGDGLAIARLLMEKGFSVTIHILEFGHKGTEDFQTNLARLHQFP
                     NADIHFVQSAQNFHHFPP"
     gene            complement(23285..24547)
                     /locus_tag="PLO70_05535"
     CDS             complement(23285..24547)
                     /locus_tag="PLO70_05535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ73954.1"
                     /translation="MKTFLYIVLLAFLSYCCNTSTRDKKPGEEDSLQYYPVTPEKIDQ
                     QEFREYYRRLTAFFDTTLLRNNNFNGSLLIAKSGTIIYERYIGKADLRKKDTISENTP
                     LHIASTSKPFTGIAVLRLIQEGKLSLNDTITKYFPSLPYPGITVKMLLNHRSGLPNYV
                     HFMDKTSWNKKDYATNKDVLGIMIKDKPNINFQPGKRFSYCNTNYILLAMIIESITGK
                     SYPDYMQQKFFLPLQMKNTYVFTLKDTLTATPSFNYNGNYWDYDFLDGTYGDKNIYTT
                     AKDLLKWDQALYTFQLLNKSLLDSAFAPYSFEKPSVHNYGLGWRLQLLPNGKKIIYHF
                     GKWHGFNAAFARLTDEKVTIIILGNRFTRNIYNTAHLCYDIFGNYRQEVIKDEEETDS
                     VSSIKTGLPALKNTKFPSGKNTRKNVKN"
     gene            24722..25963
                     /gene="serA"
                     /locus_tag="PLO70_05540"
     CDS             24722..25963
                     /gene="serA"
                     /locus_tag="PLO70_05540"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQZ73955.1"
                     /translation="MTKAGNTTSYPKEKIRILFLENISDTAVKNFRHHGYVRVDKISK
                     ALTEEQLIEEIKDVHILGIRSKTQVTKKILDAAKKLQAIGCFCIGVNQVDLKSATKNG
                     VVVFNAPYSNTRSVAELVIGAAIMLIRRIPDKNKAAHEGIWMKDAKGSYELRGKTLGI
                     IGYGNIGSQVSVLAEALGMKVIFYDVETKLPLGNAEDAKSLKELLSRADVVTLHVPET
                     NQTKNLISKTHLKHFKKGAILLNYARGEVVDLEALRKEILEGHISGAAVDVYPWEPEK
                     NGDHFQTPLQDLPNVILTPHIGGSTEEAQQNIGEDVSVKLFNYLEKGITFGSHTVPAL
                     ALPPQEGSHRILHIHNNVPGVLSAINTQLSKHNINIVGQYLKTNEDIGYVVLDVDKKL
                     SSQALHLLKDVKATIKVRMLY"
     gene            26087..27379
                     /locus_tag="PLO70_05545"
     CDS             26087..27379
                     /locus_tag="PLO70_05545"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQZ73956.1"
                     /translation="MKVVIVGAGFGGLRLARKLNNKAGFEVVLVDKFNYHQFQPLFYQ
                     VATAGLDASNISFPLRKVFQKSKNVRIRLADLKSVVPDENKIITDIEEINYDVLVLAT
                     GADTNFFGNAELAAHAFPMKSTVEALQIRHRLLHLFEEAHTITDEDELQRAMNIVVVG
                     GGPTGVEVSGALAEMKKYVLPKDYPELDFTKMNIYLLEGTGKTLASMSQKSSEKSQQY
                     LEKLGVNVLTATLVKNYDGKNVELQDGNRIPAATVIWAAGIKGNIPNGIDHSLIARGN
                     RIKVDRYCKVQGLQNIYALGDLAYMETPGFPHAHPQVAPAAIQQADMLAHNLLLIEKK
                     ANTDRLYAFEYYDKGAMATVGRNLAVVDVPKPKLHFSGFFAWLIWMGLHLMLILGVKN
                     RFFVFMNWLYNYVTYDQNLRLIFREFNREESIKKNFKI"
     gene            27376..27816
                     /locus_tag="PLO70_05550"
     CDS             27376..27816
                     /locus_tag="PLO70_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73957.1"
                     /translation="MKWVVPNWLIISGAIGQIFTALIYPYIRHVVFDWYNDIKKLKPL
                     NQEIARTYGRYIQGLNFSFGLISMLFTEDLKNQTGLAIALTGLIAAYWIGKVITQFAY
                     YPMYEIPRKLIFKIGEVGMNILFISFAVIFSWLFIYNLSGYLKG"
     gene            complement(27823..28332)
                     /locus_tag="PLO70_05555"
     CDS             complement(27823..28332)
                     /locus_tag="PLO70_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73958.1"
                     /translation="MNLRETILAEHSKANCTKIVKWIGSNQQRFDSLFELFLHDDPLM
                     AQRASWPLSYAVIAHPVFIHKHLARLLKNLKKPKLHDAVKRNTVRLLQEIAIPERFRG
                     NIMNTCFDYISSPDEKAAAKAFSLTILHNLSKQYPEIKQELKTIIDDRWEYESAAFRA
                     RARKILKEL"
     gene            complement(28335..28886)
                     /locus_tag="PLO70_05560"
     CDS             complement(28335..28886)
                     /locus_tag="PLO70_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73959.1"
                     /translation="MQIHRNLLLISLLVIIAGCNGIATKAETDEVPNEKNETSKLNTD
                     LNSNNKSSSVTLPDSTSLQPTTDPNIILANIDQFLVSRSIIPETTVNTEGISGAMVTI
                     QNSLNFSFQKIIIEVSILLADDKEYRTDYYTLYNIEPASTKTIKIPNTSRGVKIVSHV
                     IKVKSNELTNGEWILTGNRFVPK"
     assembly_gap    29345..29444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29586..30965)
                     /locus_tag="PLO70_05565"
     CDS             complement(29586..30965)
                     /locus_tag="PLO70_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73960.1"
                     /translation="MKSLFLLLTLSLSAFFATAQNYDNIKTIMMLNQLDKAKADFDKA
                     FTNAKFAGKPEAYIMKTAIYAGLAMSDAKKNTPESEQLTAEADAAFSKYKEMDPTMAL
                     ISDPTYQNGPINLYSSYYTAGYNEYSAKKWQTSLVKLKKAIEYSDLLILKKILSSPVD
                     TNVLILAAITAENSGNKEDAASYYTRLADAKIGGDGFEGVYRFLVSYSFAKKDMSAFE
                     KYKALGKELFPKSEYFNYDKIDFAVGLMTNFNDKLKSLEEVLATDPDNYKANQVLGEI
                     IYDTLNPKEDAPQDPLPNAVELEKKMKEAFEKSAKAKPETAEIPYLYMGDHFINKASK
                     VNDRREAHSKDMKARTKPGAMSSKEDIAKRDALDKEYGETLEQAREPYEKVAAIFANK
                     VKQENELEMRDKQQYKKAVSYLADIFAFKRAMAKGKPADQAKYSAEEKKWTDAYDAIN
                     SIKEKKRDH"
     gene            complement(31072..>31626)
                     /locus_tag="PLO70_05570"
     CDS             complement(31072..>31626)
                     /locus_tag="PLO70_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQZ73961.1"
                     /translation="KSGRAIRFSEEKVRATGRGAIGVAGIEVEDNNDEVVGMICVNPE
                     TDASKTVLVVSEKGFGKRTPVDEYRFTNRGGKGVKTINVTDKTGSLVGILDVAETEDI
                     MITCKSGVTIRMPVTGISEQGRATQGVKLIRLDEGDEIAAITNLDDQEHNGHGDSTSE
                     EIQNGEEGNATGTGENPSTESATD"
BASE COUNT         9151 a         6346 c         6418 g         9511 t
ORIGIN      
        1 cgtaaaaaat ttctgtttac ccctgatttt tatctcaatg gataaacaat aactgccaaa
       61 agtgggatag ccaatatctc atgttcattt tataacttca catcaaatta tcagtatgca
      121 ggttttactt tcatcacagc aacttgctga tactataaaa gtgctgtcgc aacaactgag
      181 taagagctac ccttcgcttg agaatatagt ctttgtgggt attcagcagg gaggagtagt
      241 gatagctaat gatattgtag cgttattaca gcagcagcat tcttcagaaa aggttcaatt
      301 tggacaattg gatatcactt tttaccggga tgacatccgt aaagagattc ttgcccccga
      361 tactatgaat attccttttg caattgaaaa taaaattgtt gtactgatcg atgatgtttt
      421 atttaccggc cgtactataa aggctgcctt agacgctctt ctcgattatg gcagacccgc
      481 caaagttgaa ttgtgtgttt tgatagacag gaaagagcat cgccagtttc ctatacaggc
      541 tgattatgtg ggggcaacag taacaacaaa aaaaacagac aaagtaaagc tggtaaacag
      601 ggaagtggtt ttgatacctg cataaactgg cttccattca tatttcagcc attctattgc
      661 aaaaatatta ttcggatcgg gcagaatgcc ggaaaacaaa cattttaccc taatttcgct
      721 ttttaatgca actatccact cgtcatctgc tgggcattaa aaatcttacc aaagaagata
      781 tttccctcat tctatctacg gcagagcaat tcaaagaagt tttacagcgg cctgtcaaga
      841 aagttccctc tctccgggat ataacaatcg ttaatttatt ttacgaaaat tctacccgta
      901 caagattttc ttttgagttg gcagaaaaaa ggttgagtgc tgataccatc aattttgcgg
      961 catcctcctc ctcagctgca aaaggggaaa cattacttga tacagtaaac aatatcttat
     1021 ccatgaaagt ggatatggta gtgatgcggc acagtgccag cggagcgccg catttccttt
     1081 cacagcatat acccgctgct atcatcaatg ccggtgacgg aataaatgaa caccccacgc
     1141 aggctttgct ggatgctttt tctattaaag aaaagacagg ttcactggaa ggaagaaaag
     1201 tggctattat cggggatatc atgcatagca gggtggcaca aagcaatatc tatttattga
     1261 ataagatggg agccaaagta actgtttgcg gcccgcctac attaatacca aaatacatca
     1321 gtgaagcttt aggcgttaac gtaagttata acctgaggga aaccttagaa tggtgtgatg
     1381 tcgctaatgt actcaggatt caactggaaa ggcaaaacca ggtattattt tcttctttgc
     1441 gggaatacag tatcgcttat ggcataaaca ggcagttact cgatagttta caaaaggaaa
     1501 ttgtaatcat gcacccgggc ccgataaaca ggggggtgga acttgacagt gatgtggctg
     1561 atagtaagca atccattatc ttacagcagg tagaaaatgg tgtggcagtg agaatggcgg
     1621 tactttactt attggcaaac agaagttgag tgaaaataaa tttagatttt agatggcgat
     1681 tgaatacaat catacattta aaatcagcaa ccaaaaatcc tactaatgcg aatctgcttg
     1741 tttcccggaa cttttgaccc gctaaccctt ggccacgtgg atatcataaa cagggcattg
     1801 cctttatttg ataagatagt ggtgggtatt ggtttaaatg cttccaaagc cccaatgttt
     1861 tctcctgaac aaaggttaga atggataagg gagatttata aagatgagga aagggtggaa
     1921 ggagcggttt atgaagggtt gactattgat tattgtaaaa aaataaaagc acattttatt
     1981 cttcgcggga tacggtatgt aagtgatttt gaatatgaga aaacaattgc agatgctaac
     2041 cgcactttag ataaatccat tgaaacgatc tttcttaccg gtgaacctaa gtatacttct
     2101 gttgcttcta ctattgtacg tgatattatc aggaacggcg gaaatgcggc gcctttttta
     2161 ccagatgtgg tttctaaatc gcttgtaaaa tagccttatg tccatctggt ttaaaaaaga
     2221 cctgacaatt gcggatatca gccttcttgg aaagcaaacc atgagtgact atattggtat
     2281 cgagtggaag gaactcgggg aagatttttt aaaagccacc atgccggtag accaccgtac
     2341 taaacaacca tatggactgt tgcatggggg tgcgtcatgt gtgctggctg aaacaatcgg
     2401 cagtttggca tctgcgatgg tggttgatca ctccaggtat ttttgcgtag ggctggaaat
     2461 taatgctaat catatccgca gtgcgaggga tggatttgtt accggtgttg cccggccttt
     2521 gcatatcggg agaaatacgc atgtctggga tattaagata tatgacagga gtgaaaaatt
     2581 agtttgtgta agccggctta cagtagctgt tatagcaaga aaagataccg ggtcagtgtg
     2641 atgatatcat ttctaaaaca tccacaacag atgggaatga attcttaata tagtcaacca
     2701 gttcaccagc cttaacccat ttaatctcat gaatatcttc ttccatttgt ggaatgagtt
     2761 cctgttcccc ttttactttc attttgaacc aatgtgattc tttaagaata aacctcgatc
     2821 cctcatgata agtgtgatat gtaatggtga gaggcgaaat caattttata tgctgtaatc
     2881 ctgtttcttc tgttacttcc cggacagcac atgtttccag gttttccccg gcatctaatt
     2941 tgccttttgg caaatcccat tttcctctcc ggaagatgag taatatttct tttttttcgt
     3001 tctgtactaa tcctccggct gcaatgatga tcgtaaactt tttataaaag gccttcttta
     3061 attcttccag gtcaggatgg aaaaagacac cggcatgcac tttctcctgt tccatttcat
     3121 ggatcattga ctttatagtg tgggtattca gttcatcaat aaacactgca tcatcatgat
     3181 gaatgtatgg ctgaattgtt tcatctacat tatcgcagag gaaaagtggt ttattattaa
     3241 agtaaatttt tatatacata gtgccttgtt cattttatct tcgcttcatg acagatgcaa
     3301 aagtagtagc cgaaaaactg cttcaagtta atgcagttaa gttaaatccc gaacaaccat
     3361 ttacatgggc aagcggttgg aagagcccca tctattgtga caatagaaaa gtcctgtctt
     3421 tcccgtttat cagggattat attaaatcgg aaatgtgcaa tgttattttt gatagatttc
     3481 ctgaggctga gttattagca ggtgttgcca ctgcgggaat cgcctgggga gctatggctg
     3541 cagaccagtt aaagctgccc tatgtttatg tacgtcctaa accaaaggaa catggcctgg
     3601 ggaaccagat cgaaggtttt tatgaaaaag gtcagaagac ggtggtaatt gaagaccttg
     3661 tttccaccgg gaaaagcagc ctccaggtcg tggatgtttt gaaagaacaa gggctggatg
     3721 taatagggat ggtctctatt tttacatacg gcttcccggc tgcaacagaa gctttttcca
     3781 atgcatcatt gacctatcat tccctcactg attaccctac tttaattgaa ctggccgtgg
     3841 agaaaggagt cgtttctgca gggcaacaag agattttgtt aaaatggcgt gatgatcctg
     3901 cagggtggaa agggattttg taatttcatt tatattttaa gagctgatga aaaaatatgt
     3961 aataattgct ttttgcctga taggtattgc cattgccgga aatgctcaaa caaaaccagt
     4021 tgttttagct acgatcaaaa cgcccaatgc cctttgcccg atttgtaaga caaagattga
     4081 aacttacctg aaacgttatg atggtgtaat ggaaatcaat gtgaactaca gaaaagggga
     4141 gaccagggta aaatatatta ccgaccggac agatattgag caaataaaga ctgcaattgc
     4201 caataccggc tacgatgcag atgatgtgcc tgcaacagaa gaagcttaca accggttacc
     4261 taagtcatgc aagaaatttg aagatggtgg agggcatcca aaaccaaaag cacctcctgc
     4321 tcaactccag caatagtatt cagcccttct taagcaaaga ggggcttttt tatttcaaca
     4381 aaccgctaat attttgtttt ccctcgtatc gtaccgggta attcacataa aatccggctt
     4441 ttccaatcct ggcttgcccc tcaattttca gcatcacctc atttttaagg aaaacagcta
     4501 acgagttttt taatacactt ttcatttcaa gttcaagttt taccggcaac ctgaaatcag
     4561 ccttagcggg aatgaggata agggtatcca taaaaaaccg tccgagtgga gtaccatcaa
     4621 tccatgcatc tccttcggca tttttcaatt tgatctgtgt gttgtttggg ttgaaataat
     4681 gcaggtcaat ggccagggat gtatgggaca ggcttatcgc cgttgttttc acattttcaa
     4741 tgcctttaaa atcaggttcc cggaaagagc tgcaggacat gaggagaatt gtgatcagga
     4801 aagttcccaa aaaggccata gcgtagtgct gtatgttatt ggtataaaaa aataataaga
     4861 tgcaaaatag gggaaaggcc acttacaaag agttaaaacc ttcttttcag gtatattcaa
     4921 tatgctatta tacttagtta tttgcaataa atctactagt tacttattac taaaaatagt
     4981 agtgttttta tttacattaa atatatggta attaaattat tatactgaat atcgaactat
     5041 tatatagaaa atatttaagt acaaaatata tttattcgtc aaattattga tgagggcaat
     5101 aacaagcttc aataatatca tatattaatg aaaaccaatt tgtaagcaag tttatttata
     5161 atattacatt gatttaattt aaaaattaat taattacatt atatgagtat gaaaatagga
     5221 ttcttatgtc ttgaaagcat agccaaaaat tggtaagtct gaggttaaat tgatatgaac
     5281 gggtgataaa aaaacttcat aagtcattca ttatcgtaca actacgagct tcggtattta
     5341 cggtttttat tttttctatc tgtctgaaaa gcttcgattt acaaattctt tgttttaaat
     5401 tagcaccggt ttcttcacct tggactttgg actacatgag aaaactacac ttactaagta
     5461 cccttttact tgccattttt actggaggta tttcctatgc acaggacttt tcaaataagg
     5521 ggaaggactt ttgggttggg tatggctatc accagataat gtctgggact aatctgcagg
     5581 atatggtcct ttattttgcg gcagatcaaa atactaatgt tacagtaacc attccgggta
     5641 ttggttatgc tcagaattat tttgtggcag caaacactgt attaacttca agccctatcc
     5701 ccaaaacagg tcttcaggat gccaggctgt taaccgaatc aacaatacct gaaaagaaag
     5761 gcattcacat cactagtgat aagcctatcg tagcttatgc tcatatatat aatcaaagtg
     5821 tatcgggtgc cactattctt tttcctacaa ctacactagg caaagagtac ttttctgtta
     5881 attatacaaa catttcgaat tcacctaatt caaactgctg gttttatgta gtagcagcgg
     5941 atacaggaac tactactgtc gaaattactc caagtgccgc cacaattaac cgcccggcag
     6001 ggattccgtt ccttgtcacg ttgcagcaag gggaaatata taatattatg gggcaggttt
     6061 ctggcaatac gggtgttgac ctaacagggt ctaagattca aagtataaat acaggagctg
     6121 gttgtaaaag aattggagtt ttttccggca gcggtagaat cagtataact tgtaacggca
     6181 tttcatcttc ttcggatagt tatatggttc aggcttttcc gaaatcagca tggggtaaaa
     6241 aatacctcac cgcttctgca agtggcggaa tggataacaa tatttaccgg gtatgtgtta
     6301 ccgacccttc tacgatagtg actgtaaatg gtgctccgat tgcagtaccg ctgcagaacg
     6361 ggttttatta tgaattaccg attaccccga gtccaatgaa aattgaagcg aataacccaa
     6421 tattagttgc tcaatatttt acttccacac aaggaaatac cgggtgtgga aatgctggtc
     6481 ctggtgatcc tgaagtaata tacctaagcc ctgttgaaca gaatataaat aaggttttat
     6541 ggaatgcaac cccaaacttt gcaatcggta gtcacttttt taatgtcatt gtcccaaata
     6601 caggaactgg attaagttca ttcaggttag atggcatcct tgtttcccca tctttattta
     6661 ctgttcatcc acaggatcca gcttattctt acctgaaaca atcggttggt tcaggtcaac
     6721 atacaataca gtctgattca ggcttcaatg ctattgctta tggttttggt tcatttgagt
     6781 cgtatggcta caatgccggg accaatatta aagatatata tcaatatatt tctgtacaga
     6841 atccacaggc aactgtgaat tttcctgcaa cctgccgagg aacccccttt tatttttcca
     6901 tgactttccc ttatcagcca acttccattc aatggcagtt tggcccggct ttaaatgcta
     6961 tggggatagc tgatgttaat atccctgctc ctgttcccac atctacaact gttgtgaatg
     7021 ggaaaacact ttataactac caactaccaa caccttatgt aataacggtt gcaggtacgt
     7081 acccaatcag ggtagtggct accaatccaa cccccgatgg atgtggtggt acccaggaaa
     7141 ttgattttga tttacaggct ttcgatccac cattagcaga ttttaatttt acaactaatg
     7201 gttgtgtatc cagcccggtt agttttactg ataattcaaa taccaacggc cggccggtta
     7261 tttccaggca ttggaatttt ggcgatgcca atacttctgc tttaaataat cccagtcata
     7321 cgtatgcagc acccggcgcc tacacggtga aatattcact gataactgat attggctgca
     7381 ttgctgatac agcttcccgg gttgtaacca taaacgatcc tcctgtggct ttatttacag
     7441 ctgcagcacc ttattgtgaa ggccggtctg ttactttcac cgatcaatca acttcacccg
     7501 gaggagcaac tatcagccaa tggacatggg attttggtga tggttcacct gtagtggtaa
     7561 gaaatacgag taccaatgaa acccatacct acgccagtgc cggaagttat acagccacat
     7621 taagggtagt tacctcttcc ggttgtcaaa gtacattatt tagttttcct gttactattt
     7681 atccaaaccc ggttgcggat tttaatttac cagttgcttg tttacctgtt ggtgcagcgc
     7741 aattcaatga tttgtcaact gttggtggtg gtaatactat caccagctgg gcatggaatt
     7801 tcggtgatgc aggaacttcc acggttcaaa acccctcaca taattattca gccccgggac
     7861 catttaatgt gagccttact gttacatcta ataacggatg tacggataca aaaaaccagg
     7921 tattgaatac ggtgtatgca gaaccacagg ctgcattcaa ttcgttgccg gaagtctgta
     7981 tcggaagcct gattggattt tcagatgcca gtacggcccc cggaagcaca gtgacggggt
     8041 ggagctggaa ttttggtgat ggctcaccca tctctaccat acaaaacccg acccacattt
     8101 tttctacagc cggaacatat acagtcactc taaatgttac ttccgctgcc ggatgccaga
     8161 caatgaataa ttcagcaacc cgtacagtag tggtaaaacc attaccaaca ggttcaatca
     8221 ctggtgctat tgccgtttgt ttaaatgcac cgtcacctaa tgtaaccttt acaggcggaa
     8281 caggtactgc accttttaca ttcacttata ctattaatgg cgggcctgta caaacagtta
     8341 taacaacaag tggtaattct gttgatgttc cggttcctac caatacagcc ggaaccttta
     8401 tttataccct gacaagtgta aaagaaggca gttctgccag ctgtgtacaa agtcaaagcg
     8461 gatcagtgac cgtaactgta aatgctttgc ccactgccac aatcacaggc attactacgg
     8521 tttgtttaaa tgctgcttca ccggctatca cttttaccgg tgcaagtggc tcagcgcctt
     8581 atacatttac ttacaacata aatggagggc ccaatcaaac tgtaacgact accacaggta
     8641 attcggcaac tgttactgtg cctaccacta cagccggtac ttttacatat aacttaatta
     8701 gtgtgcagga aggaagtgct aatttttgca accaggcaca gattggctct gcggttgtaa
     8761 ttgtgaatcc attgccaact gcaagtataa gcggcgataa tgaagtttgt cttaattcag
     8821 cttcacccaa tattacattt accgggggag ccggctcagc accatacaca tttacttaca
     8881 atattaatgg cggtccaaat caaacagtga cgaccaccac aggtaatact gtaactgtac
     8941 ctgtaccaac cactgtagcc ggtacattta catacaactt aattagtgtg caggaaggaa
     9001 gtgctaatct ttgcaaccag gcacagattg gctctgcggt tgtaattgtg aatccattgc
     9061 caaccggtaa ttttactttt acggctccca gttgcgaaac aagaaccatt acgttcacag
     9121 atgcttcagc cgccaatgca ggaactatta acaactggca gtggaatttt ggcgatccgg
     9181 gaagcggggc gtccaacaca tcaaccttac agaacccatc gcatacattt gttgcggcag
     9241 gaacttacac tgttacactt acggtaacaa cagataaagg ttgtgttagc aatgtttata
     9301 gcaatccagt tacgatcaat gcgagaccta aagcaggatt tatatcccca gaggtttgtt
     9361 taacggatcc attagcaaca tttacagata ccagttcggt ggttggagga acgattgtgg
     9421 catgggagtg gaactttgga gatcctaatg ccaatgccgg taaccccaat acttctgttt
     9481 tacaaaatcc aactcaccgg tacacagttg taggtccgta taccgcgcag ctgattgtaa
     9541 ccagtaacaa tggatgtaaa gacaccacac agcaaacatt tattgtaaac ggctcaatac
     9601 cggttgccgg gtttaccgtt ttgaatccgt caacgctttg tagtaaccag tcagtaagta
     9661 taactgatgc atcaacggtt gactttggaa acctggtaaa ggttgaggtt ttctgggatt
     9721 accttaataa ccccaccatc aagacaacag atgataatcc aataccaggt gcagtttata
     9781 cacatagtta ccctgaattt ggtacacctt tcacacggac attccaggta aggtatgttg
     9841 cttattccgg tatcaattgt gtaaatacaa caactcaaac tataactgta ctggcaacac
     9901 caacattaca gtttgcgccc ataccatttg cttgtacaga taagccatca ttccagatta
     9961 cacaggctca gctactgaat ggacttccgg gggcaggagt gttttccggt acgggtgtta
    10021 caccaaccgg tttatttagt ccctctatcg gggcaggtca gtataacatc aggtacactt
    10081 atacaggtac aaatggttgc agtaactata aagagcaaac tgttgacatc tatacagcac
    10141 ccgcaataaa tgccgggcca gataaatttg tattagaagg cggacaggta acacttacac
    10201 cagcattgaa tgttggggtg cctttaacgt acatgtggac tccgccaaca ggactggata
    10261 atgttacaag ttctttcccg gtagcttccc cgacagatga tataacttat acattgactg
    10321 taacgacacc ccagggctgt actgccagcg acaatgtgtt tgtaaaagtg cttaaagcac
    10381 cgatcattcc taatattttc agcccgaacg gggatggtgt acatgacagg tgggacatac
    10441 cattcctgga aagctatccc ggatgtacga ttgatgtggt taaccgttat gggcagttaa
    10501 tattccgctc agtagggtac accacggcat gggacggaag agtaaatggt aaggatgtgc
    10561 cggttggtac ttactattat gtaattgatc caaaaaatgg ccgggccaaa gtaaccggtt
    10621 atgtggatat aatcaggtaa tgctatgaaa cgattactat taactgttgt tattgttttc
    10681 tgtgcaggtc ttgttgcgga tgcgcagcaa aagcctcact atacacaata tgttgtaaac
    10741 ccttttatta ttaatcctgc cattgcgggt attgataatt atgttgattt gaagatggcg
    10801 gtaagagacc aatgggtggg aataaatgga gctcctaaaa cgacatattt aagtatacat
    10861 ggaccactgg gtaaaaatga ttaccgtact tcctctacct ctttccaggt accgggtgaa
    10921 aatccaagag gaaaagccta ctgggaaaat tataccgctg cagagccaca tcatggcata
    10981 ggtttaagtg tcatcaatga ccggacgggc agtttcaatt tcttctcagc cagcgccact
    11041 tatgcctatc atttgggact aagtccaact accaatcttt ccggggggct ttctgccggg
    11101 attacaaaag ttagtattga cgccagtaag catgattttt caggtaccgg tgatccgagt
    11161 gaccctgcaa ctggttccgg tttcagcagc gaacttacca ggataaggcc acagatggct
    11221 gctggtttat ggctttattc aagggattat tttataggat tcgcagcaca gcagatactt
    11281 ccacaaacgc tgaattttgt agatgataat gcttcaattc ttaccaaggg gagattagtg
    11341 cctcacttgt ttttaacagc tggttaccgg tttttattat cggatgatat caatgctatt
    11401 ccttctgtaa tggtgaagta tattaaaggg agttcattca atgaatttca acctgaagta
    11461 aacctgaaga tgcaataccg tgatcagctg tgggttggag gaagctatcg ctaccaggat
    11521 ggttttgcag gcatgctggg acttaatgta agcaatacct ttaatgtaag ttatgcttat
    11581 gataagtcaa gtaataataa tatcctccgt gatttcaaca gcggtaccca tgaagtagta
    11641 ttcgggttca tgataggaaa taaatatagt gaagcctgcc cccggtgcta ttaatgtcaa
    11701 ttgtttgtat ttatagggat aaacctgacg attttttgta tttttctttc ctccgtttaa
    11761 accttcctga aagattcttt cttattcttt aataattgca gtttatactg ctatggctta
    11821 tgagaggggt ttatattttt ttgtgcatac ttctttttct gtcgggtact tttatgttat
    11881 ccgcgcagga ttattctaat aaggggaaag atttctggat tatttatacc ggtcattacg
    11941 atggaaccaa ttcacggatg gccctgtata taacatctga tctgaatgca tcaggaacat
    12001 tatctgttgg tggcagtacg atcaatttta ctgtaacagc taaccaggtc acgactttac
    12061 ggttaacaaa tgcgacaata ccgtcaaatt cacttgcata caatggtcag acaattggaa
    12121 ttggtacaaa taaaggaata catattgtta cagatacccc ggttgttgtt tattcgcata
    12181 ttttaaatgc agccaggtcc ggatcaactt tagtgttgcc aacaaaagtg ctgggcaggg
    12241 aatattatgt ttcttcgtat gcttcaagcg gcaacttaac tccccgatct tcagaatttg
    12301 cagtagtggc taccctggat aatacaactg tagaaattac tccaaaacaa gcggatgcta
    12361 ataatacata ccctgcaaat attcctttcc aggtaacttt aaataaaggc gatgtctttc
    12421 aatttcaatc tgccaatggc ggggatttaa ctggttcgtt tattaaatca atagcagcgg
    12481 ccggtcaccc ctgtatacct attgctgtat tttctggttc cacatggacg gccatgggat
    12541 gtgccggtgc cggttcggga gataatcttt accagcagtt gtttccactt gtttcgtggg
    12601 ggcaatatta tatcaccgct ccgttcaaac ttcgttccta tgatattttt cgtatcatgg
    12661 ttaaagaccc gacgacggta gtccaggtca acggagttac gttaaatcct gcaatactga
    12721 ttaataactc tttttatgaa atcaatacaa taggggacaa tactccgaga ataatcacat
    12781 ccgataaacc agtttgtgtg gttcagtata tgatcacaca atcctgtgac ggtgttaaca
    12841 gtgatcctga aatgatcatc ttaaatccca ttgagcaaac cttaagtgat attactgttc
    12901 tttctgcccg gaacgatcta acacctccta atactaacat cacaagacat ttcttaaata
    12961 tcattattaa aaccgctggt ttaggaagcc tgaggatcga cggggcacca tatacatcaa
    13021 cacctgtggc aattccgagc acagcctatt cttatctgca agaagaagta acggcgtcca
    13081 ccaatattaa tccttctcat agggtttatg ccgacagcgg atttattgct atcgcctatg
    13141 ggtatggaaa tgtagaatcg tacggttata atgcaggaac aaatgtgagg gatttgtatc
    13201 aatttgtttc tgttcagaat caatatgcta cagtgccatt cccggctgca tgcaaaagct
    13261 ctccgtttta tttctcaatg gtatttcctt atcaacctac acagatagcc tgggacttta
    13321 atggcttatt ttcaaacgtt accatcaata atccggttta tgattctaca tggttcgtta
    13381 acgggagaca gctgtatcgt tatagattaa ctgccccgta tactattaat acagcaggta
    13441 cctatccaat cagggtaatc gctcaaaacc ctacgccgga tggttgcggc ggagaacaag
    13501 aaattaatta tgatctgcag gtatttaatc ctccggttgc tgatttttct tatacccctg
    13561 cctgtttccc taatcctata cagttctctg ataactccaa tacgggtggc agaccagtaa
    13621 taagcaggta ttgggatttt ggggatgcta catatgctgc tgtgaataac ccttcacata
    13681 tttatgctgc tacaggtact tacaatgtgc gatacgcatt aataactgac gtaggatgtc
    13741 tgtctgatac aaccatgcac ccggtaacac tttctccact accaactgcc tccatcagcg
    13801 ggaccattga agtttgtcaa aatgcaccac cacctgttat tacatttacc ggtgctgttg
    13861 gtactgctcc ttatactttt acctatacaa ttaatggagg accaaaccaa tttgttacaa
    13921 gtactggaaa tactgcaaca gttccggtac caacagctac tgcaggaact tatatatatg
    13981 caatagcaaa tgttcaggat gcaagtcctg ctgtttgcaa ccaggctcaa actggttctg
    14041 ctattgtaac tgttaatcca ttgccgaccg catcaataag tggaagtacg gctgtttgta
    14101 aagatgctcc ttctcccaac atcactttta caggggcaac gggtacagcc ccatatactt
    14161 tcatttatac tattaatggg ggtgcgaatc agtctgtgat aacaaccagc gggaattcag
    14221 taaccgttcc tgctcctaca aatgcagtag gaacatttac ttataatttg gttagtgtat
    14281 ctgatggaac agcaaccata tgcagccagt tgcagagcgg cactgctacc attattgtaa
    14341 atccattgcc gacagcaagt gtggcgggta ctacagaagt ttgtcaaaat gcgccttctc
    14401 caatagttac atttaccggt tcatcaggta ttgcacctta cacttttgtt tatactatta
    14461 atggagggcc aaaccaggtt gttataagta cagctaatat tgctacagtt caggttccaa
    14521 cagctgttgc cggtacattt atttataacc tggtcagcgt tacagatgca agtacaacta
    14581 tttgcagcca gctgcaaaat ggtattgcaa ccataacagt atacccgtta ccatccgcca
    14641 acttcaactt ttctgcacca ttatgttcag aatttgtggt cgacttcacg gatatttcaa
    14701 atccaaacgt tggtaacctg gtaagctggt tatggaactt tgatgatccg gggagcgggg
    14761 cagctaatac atctgcctta caaaacccgg ttcatacatt taatgcgccc ggaatatata
    14821 acgttagcct gatcgttacg aatgataaag gttgtgttag taatagtttc atcagacagg
    14881 ttgaaatcaa acctaaacca ttatcaggtt ttgttttacc agatgtatgt ctgaatgata
    14941 catatgccca gtttaacgac accagttcgg tagcatctcc gggtcttatt gcagcatggc
    15001 aatggaactt cggtgatccc ggcagtggaa taaacaatac ttcagtttta caaaatccac
    15061 aacatagtta tacggcagtt ggtacttaca atgttgaatt gattgtgacg acaagtgatg
    15121 cctgtaagga tacggtagag caaacgcttt ctgtgaatgg cagtttccct gtggctgatt
    15181 ttgtagtgaa taacccaacg accttatgcg ccaacgattc tgtagcaatt atcgaagcct
    15241 cgacagtgtt tccgggaaat atcactaaaa ttgaaattta ctgggacaat aatggccagc
    15301 ctgcagcatt tgattcggat aataatccgt ttcccggaaa aacgtacaag catctgtata
    15361 caaattttca aacgccattg acaagaacat tcaccatccg gtacagagca tactcaggtg
    15421 gtgtgtgcgt caatgatatt tttaaagata taactgtgaa tgcggcgcca agtgtccagt
    15481 ttagcgcaat taatgatgtt tgcctggatg ctgtaccctt tcagattact caagccagtg
    15541 aaacaggtgg tgtgccgggt tctggagttt ttagcggacc gggtgtatct cctgcaggaa
    15601 tttttaaccc gtccgtggca ggtccgggag ttcatacaat taaatacctg ttcacttctg
    15661 caactggagg ttgctcagac agcttaacac aatccataaa agtttttgag ccagctgtgg
    15721 cagcattcag tgtgagtgct ccgttatgtg aaagaaaagc tgttacattt acacagtcat
    15781 ccaccactcc ggaaggcata cttacaacct ggatatggga ttttggtgat gggtcaccac
    15841 tattaacagt atcaaatggt aatcccgtaa cgcatattta tactacatgg ggagcctatg
    15901 atgtaaaact aaaagtagtg accagtaatg gttgtgttag tcttgaaaaa ctactcagga
    15961 taaatgtgaa cccgcaacct aaacctgatt tcagcatacc ggtaagcctg tgtttgcctg
    16021 ctgctaacgc cacatttacc aatctttcca cgatagcaga tggcactcag aatttattaa
    16081 gctattactg ggattttggc gacccggcaa gcggaacggt gaataattct acagcgacca
    16141 acccatctca tatgtatatt acaaccggcc cctatactgt gaatctccag gtaacgagtg
    16201 gtgccggatg tgttaatgat accacgatct tgctgaatac aatccatgac cagccaattg
    16261 catcttttat tactgataag ccggaagcct gcctggggca gagtatccgc tttacagaca
    16321 atactgatcc aatgggtggt atcactaacc aatggaactg gatgatggat gatggtaata
    16381 caagaaatac ttcaactttt aattatactt attcatctgc tggtacttat aatgtgagct
    16441 tatattcatt caatagtttt ggttgcagga gtacaactta tactacacca gttgttatac
    16501 atccttaccc gactgttgat gctggtccgg acagattagt actggaagga ggacaggttg
    16561 ttctgcagcc tgttgtaacc gggaatgata tgatttataa ttggacaccg aatctctact
    16621 ttaccgggag taatactgtc ttaaacccaa ctataaatgg tgtggatgat attacttata
    16681 cgctgacggt tacggcaagg ggaaattgtg cgaagtcaga ccaggtgttt atcaaagtgt
    16741 tgaaataccc aatcatcccc aatattttca gcccgaacgg ggatggggta catgacaggt
    16801 gggagttacc atacctggaa agttatccgg gatgtactat tgatgtagtg aaccgttatg
    16861 gccagttggt ctttcgttca gtgggttatg ccactccctg ggatggaaaa gtaaatggaa
    16921 aagaggtgcc tgtggggact tattattatg ttattgatcc taaaaacggg cgcaaaaaaa
    16981 ttacaggtta tgtagatata atcaggtgat tcttttaaag gttcaataca attctatagg
    17041 cgggaagtct cagaggtgaa caatcttctt attgcgtata gctttcagtg tatttaatgt
    17101 ctgctgtact tttgttttct tttcttctac agttttcaag gaaatatctg tatcgggtat
    17161 actttttact ttattgaggg aaagctcttt ttcaatcgca ttcagtttaa cagccacatc
    17221 gttgtatact ctttgcaggc tgtctacccg tatctggttg atagcattcg agagctgatt
    17281 gtttactttg tcccaatcaa tattatcgta cgcaagcttt agtttatttt cccatttgtt
    17341 ccagtcaaat ttatccagtt ctttttcata cacagatttt atttcctcct tttccctttc
    17401 tgaaaaagca tctgctatac ttttttctac caacttccat tgagaacttt ccaatacttt
    17461 ttttgatgca gccaaagcgt cctttacctg ctgttcctgg tattttttta agacaggaat
    17521 atttacttct tcaaaacctg ccagtttgta ggccaatgaa gaaatatcct gcaggttttg
    17581 ttcgctgatc aatggggtgt tagggggtgg ttcaattggt gttgccggag attgaacatc
    17641 agtagttgca gccagatttt ttgaatcaaa aatagttaaa gctgactttt tatttcccgc
    17701 ttcagcttca tcattttgtt caccggcacc ctcagtaatg aaactaaatg gcgaaaaaat
    17761 ttctgagtat gcagttgtct tattcctgtt ggattcttta ccgggtaata atagtaaaag
    17821 gttcagtgca agaatgcaga taatagctga cagcaatccg gctattttat tggctgacag
    17881 cgtttgtttt ttttgaatgc ctatgatagt ttcaatgcgg tgcagcaggt catttttatt
    17941 accacccgct gagatgatga gtgccttttg ctgatagttt gttttttcca ggagcagcag
    18001 ggcagaagca tattgatgtg aatcatactg gaactgtaat accatttcat cacagctttt
    18061 ttctctttct ctttctacaa tcttcacaaa tgcttttaca aacgggttaa agtaaagaat
    18121 agtttgaata aaattgatca ggaggttaac caggtaatcg tatctcctga tatgcgccag
    18181 ttcatgcaat aatacagcct ccagttgatg cggtgttaaa tgactgatag ctgctaatgg
    18241 tacaagaatc actggtttta aaaaaccgat agttacaggg gatgacacaa attcagaaac
    18301 ccatatctga acaggctttt taatacccat tctggccgct acattgcgga cgaatatttt
    18361 ccagtccaca ttgatcttgg taagtccgta tttcctgatt acctgcacat aacgatagtt
    18421 gcggataaaa cgtaaaaccg gaagggttaa taataaaaga taagctacag aaataaaagg
    18481 aagactttgc aacaaccagc tatatgttcc gctacttatg gtaactgact caccaacagc
    18541 ctcacctgta attattttgt tataaacgat aataaatgtg tagacgaacc ataaaaaccc
    18601 tgcgaaaagc aggcccgaag ccaaaatgct cttagatgaa ttctttaaat ttttacttat
    18661 cgcaataact atcatataga taacccataa aagagccatt tgccagaggc tgttgagtac
    18721 agcccagccg agtgattgca gtaagtgcag ctggatgccg gtcatagtta ttgtttttta
    18781 agactatcca gtaaggcctg gattttttcc aattcctcag gacttgcctt atggctttca
    18841 tcacccaaag cctgtatgac gagttgggtg ggggagcctc caaaaagatt atcaattatt
    18901 ttacctaaca ggtgcttttg tgtcttttcc ttattcacag cagcctggta tacatgggta
    18961 cgcatcgaat catccctttt aactatgcct ttttcattca ttatctgcat cagcttaaga
    19021 gttgtggtat aaccaacatc tttggtctgg gccagttctt catgcacatc cctaaccgtg
    19081 gctgttcctt tcgtccagag gatctggaga atttccagtt cgctttcggt aggctttatc
    19141 aattttggcg ctgacatagt attacgattt tgacccgaat atacgactaa tttcgtatga
    19201 taaacacaca gtaactattt ttaggctttt ttaacacccg ttaaggatgg gacgttgaaa
    19261 tagggaaata gttacgcttt tacattgatt tttttcaatt agctattttt ttaaataaaa
    19321 tattcttagt gaatctgtaa ggattttaaa tcccgtaata aaaattatat ttcctggttc
    19381 gtaattacat tggcaacgag gtaacaaaaa ataagcccca ctgtagaaac agcgggactt
    19441 aattaatggt tctgattaag ctcttgtatt attgatactg aacagtattg gctttgttaa
    19501 tagcggccat tggctcagat tttattctaa attcttttcc cataggctta ggggcttccg
    19561 gtaccacggg tacttccact ttctttgcat ttatttgtgc cagggtagga gcaatgacca
    19621 atgaaactat tgacatcaat ttaatcaaaa tattcattga aggtccggaa gtatctttaa
    19681 aaggatcacc tacagtatca ccagtaacag aagctttatg aggctccgat tttttgtaat
    19741 acatctctcc atttatttct accccttttt caaaagattt ctttgcatta tcccaggcgc
    19801 cacctgcatt attctggaac attcccatca atactccact caccgttgca cctgccagaa
    19861 aaccaccaag tgcttccgga cctaacagga aaccaatgat caacggagaa atgatagtga
    19921 tagcacccgg taacatcatt tttttgatag aggcctgtgt agaaatggct acgcatttat
    19981 catattcagg tttacccgta ccttccataa tgcctggaat agtacggaac tggcggcgaa
    20041 cttcttctac cattgccatc gcagcctgac ccactgcccg gatagccagt gacgaaaata
    20101 tgaagggtat cattccacca acaaataatg aggctaatac atctgcctta taaatatcta
    20161 tatgttgatt atcgggcatg gccacaccaa caaaagcagc aaacaatgct agtgctgtta
    20221 atgcagcaga agcaatagca aaaccttttc cggtagcagc tgtagtgtta cccacagcat
    20281 ccaatacgtc agttttttcc cgtacttctt taggcaattc actcatttct gcaataccac
    20341 cggcattatc agcaatagga ccgaaagcat caattgcaag ctgcatagca gttgtagcca
    20401 tcattccagc ggctgcaatt gccacaccat acaatcctgc aaattgatag gagccccaga
    20461 ttccaccggc caatacaagt attggaagga aagtggattc catccctaca gccaaaccac
    20521 caattacatt agtagcatga cctgttgatg attgtttaat gatagattta acagggcctt
    20581 tacccatcgc agtgtagtac tcagtaatga tactcatcaa agcacctacc agcagaccaa
    20641 caccgatggc gcctagtaca ccccatttgg taaacattac acctctgagc tccatggctt
    20701 ccggtagaat gaaatatact aacccggcag aagcaccagc cgttaaaaca atagatcccc
    20761 agttacccat attcagtgct ttttgcacca catctgtact gatacctgcg gaatcactta
    20821 cacgaacaaa taaagtacct atgatggaaa aaataatacc cacccctgcg ataagcattg
    20881 gtaatataat aggggagaat cctcctaaaa gatcatcacc gaaaacaaca gttgtttgct
    20941 ggcccaacac gatagtggcc agcacagttg caacataaga accaaaaagg tcggcaccca
    21001 tcccagccac atcacctacg ttgtcaccaa cattgtcggc aattgtcgca gggtttctgg
    21061 ggtcatcttc cggaatacca gcttccactt tacccaccag gtctgcacct acgtcagcag
    21121 cttttgtata aataccgccc ccaacacggg caaataaggc aatagattct gcacccagcg
    21181 aaaaaccagt taaaacttca atggtcctga tcatctcatc tgaattaaca gctgaatcag
    21241 gtgcaaaaac ctgtttcaaa atgatatata aacctcccaa acccaataca gcaagaccag
    21301 aaactcccat tcccatgact gcaccaccgg tgaatgaaac atttaatgct ttactaaggc
    21361 tggtacgggc tgcatgtgca gtgcgtacat tggctttagt agcagctttc atacctatat
    21421 atccggcagt ggcgctgaat acagcaccag caacaaatgc taccgcaatc agccaatgtg
    21481 aatgaggatt tgaagaagcc attacagcca gcagaatacc aactattact acgaaatagc
    21541 caagtatttt ccactcagcc ttcagaaatg ccattgcacc ttcagcaatg taggtgctga
    21601 tttctttcat acggtcattg ccggcatctt gcttggaaac ccaattgaac tttacaaggg
    21661 tataaagcaa tccaatgagt cccattacgg gcaccagata aatcaatttg tccataagag
    21721 ttgattctta atcaagattt aatgaaaacc ggccatttgg ccgggcggca aagatagggg
    21781 ttgagtggca aatttggaat ggggatacaa ggaattggga aattgggtca aaatggccaa
    21841 aaacctgata aacaggggct atagtgatgt ttgaccggca tcaattattc tgttgtaaaa
    21901 accgaaaagc attaccaagg aagttgatga tatcacctgc taacaaagct tccatagata
    21961 gttcttttgc tgccagatcc ccggctaatc catgcaggta aacaccaaga actgcagttt
    22021 cgctggaact ataaccctgt gccagtaaac tggttaatat tcctgtcagt acatccccgc
    22081 tgccggctgt agccattccg ggattaccgg tactgttgaa aaaaccacgg ccgtcgggtg
    22141 tggcaataaa agtatgatgc cctttcagga tgattatgga actaagctct tttgcctttt
    22201 caagggctaa ccgtacccgg tcaaaatcat tggcggcttc accaaataac cgttcaaact
    22261 ctttgggatg tggtgtaagg atagaaccca caggcaccaa attcaataaa tctttctgtg
    22321 atgctattat atttaatgcg tcagcatcca gcacaaccgg attccggtag gtatcgaaaa
    22381 tttcccgcag catcatcttg gtctctgatg cagtacctat gcccggtcca acacctatgc
    22441 cttcatactt cattagatca tcttcaagct ttgtattaaa tgatgaatta aaatcggtca
    22501 ttaccattgc ttccggaaca gcagtttgca aaatggaata accacatgat ggtatatgac
    22561 aggtcaataa cccggcacca ctccgcaaac aagctttggc agataagacc gcagcaccaa
    22621 tttttccata gctgcccgct accaataatg catggccata gtgaccttta tgggcaaacc
    22681 gatttcttgg tttatgaata gaatgaataa tagtgtcatc cagtaattca aaacggctgt
    22741 tcagggactt atagaaatcc tggtgaaggc caatatcgag tannnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nngggtggaa aatgatgaaa attttgtgct gattgaacaa
    22921 aatggatgtc cgcattcggg aattgatgaa gtctcgccag gttggtttga aaatcctctg
    22981 ttcctttatg accaaattca agaatatgaa tggtaactga aaagcctttt tccattaaca
    23041 accttgctat ggcaagacca tccccgccat tattgccttt gccgcagaaa atgccaaaag
    23101 atggggcatc gggatacttt ttcaatagcc attccacaca agcaattgca gctctttcca
    23161 tcaggtcaat ggaagagatg ggttcgtttt gaatagtata ctgatcccac aaccggattt
    23221 cctctgcaga aagaattttc atacaggtat aaaggtcaga aacaatttcc aaaaaatgga
    23281 tgacttaatt ctttacgttc ttacgggtat ttttccctga aggaaattta gtatttttta
    23341 gtgcaggtaa ccctgtcttt atagaagaga cggagtctgt ttcttcttca tcttttataa
    23401 cctcctgcct gtagttacca aaaatatcgt agcataggtg ggctgtatta taaatgtttc
    23461 tggtaaacct gttccccagg ataataatag tgaccttttc gtcagtcaat cttgcaaaag
    23521 cagcattaaa tccgtgccat ttgccaaagt gatatattat tttcttacca ttcggcaaca
    23581 attgaagcct ccagccaagt ccgtaattat gaacagacgg cttttcaaaa ctgtaggggg
    23641 caaatgcgga atcgagtaat gatttattga gaagctgaaa agtatataaa gcctggtccc
    23701 atttaagcag atcctttgca gttgtataaa tattcttatc accataagtc ccgtctaaaa
    23761 aatcatagtc ccagtagttc ccgttataat tgaatgaagg agttgcggtc aatgtatcct
    23821 tgagtgtaaa tacgtatgta tttttcattt gcaatggcag gaaaaatttt tgctgcatat
    23881 aatcaggata ggacttgcct gtgatactct cgataatcat tgccagcaat atataattgg
    23941 tattacaata gctgaatctt tttcccggct gaaagttaat attcggctta tccttaatca
    24001 taatgcctaa cacatctttg ttggtggcat aatctttttt gttccagctg gtcttatcca
    24061 tgaaatgaac atagttgggc aagccactgc gatgattcag taacattttt acggtaatac
    24121 cgggataggg tagtgaggga aagtattttg ttatagtatc atttaaggag agtttccctt
    24181 cctgtataag tcttaaaaca gctatgcctg taaacggttt gctcgttgaa gcaatatgta
    24241 gcggcgtatt ttccgatata gtatcttttt tacgcaggtc tgcttttcct atatatcgtt
    24301 catagatgat tgtaccactt ttggcgatca gaagactgcc attgaaattg ttattcctga
    24361 gcaaagtggt gtcgaaaaag gctgttagtc tccgatagta ttcccggaat tcctgctggt
    24421 caattttttc cggtgtaacc gggtagtatt gcagtgaatc ttcctctcct ggttttttgt
    24481 cacgggtaga agtattacaa caataactaa gaaaggccag caatacgatg taaaggaaag
    24541 tcttcaacag atatttatgg gtttatttac aaaaatagaa gccctgcata ccgggcaata
    24601 taatagtttt ccccagcagc ggtacaaatg ttaatacaaa acaactgtta gtgttaacat
    24661 tttccggtga gtttattgtc aaacagaaaa tgagctgaat agtacgtagt atatttgctg
    24721 tatgacaaaa gcaggcaata ccaccagtta tccaaaggaa aaaatcagga tactttttct
    24781 ggagaacatt agtgacacag cagttaaaaa tttccgtcat catggttatg tcagggtaga
    24841 caaaatttca aaagcgctga cagaagaaca attaattgag gaaatcaaag atgtgcatat
    24901 tctgggtatt cgttcaaaga cacaggtaac gaaaaagata cttgatgctg cgaaaaaatt
    24961 acaggcaata ggttgttttt gtattggtgt aaaccaggtg gatctgaaat cagcaacaaa
    25021 aaacggggtt gttgttttta atgccccgta ttcgaataca agatctgttg ctgaactggt
    25081 cattggcgca gctatcatgc tcataagaag gattccggat aaaaataaag cagcacatga
    25141 aggtatctgg atgaaagatg cgaaaggcag ttatgaatta aggggaaaga cattgggaat
    25201 aattggttat ggcaatatcg gaagccaggt aagtgtgctg gctgaagcat tgggaatgaa
    25261 agtcattttt tatgatgtgg aaacaaaatt gccgttgggt aatgcggaag atgcaaaatc
    25321 attaaaagaa ttgttaagca gggcggatgt agttacactc catgtgcccg aaacaaatca
    25381 aactaagaac ctgatcagta agactcatct taagcatttt aaaaaaggag ctattttgct
    25441 gaactatgcg agaggagaag tggtggacct tgaagcattg cgaaaggaaa tattggaagg
    25501 ccatatcagc ggggcagctg tcgatgttta tccatgggaa ccagaaaaga acggggatca
    25561 tttccaaact cccctgcagg atcttcctaa tgtaattttg acaccgcata tcggcggcag
    25621 cacagaagaa gcacaacaaa atattgggga agatgtgagt gtgaagctat ttaattacct
    25681 ggaaaaagga attacgttcg gttctcacac tgtaccagcc ctggcattac caccacagga
    25741 gggaagccat cgtattttgc atatacataa taatgtaccg ggtgttttgt ctgccattaa
    25801 cacacagctt tccaaacata atatcaatat tgtaggacaa tacctgaaga cgaatgaaga
    25861 tatcgggtat gtggtattgg acgtggacaa gaaactttct tcccaggcac tgcatttact
    25921 gaaggatgta aaggcaacta ttaaggtaag gatgttgtat tgatttttct ccacttttaa
    25981 agaattcagt gctttcacag gttcttgaag actggaaata ataaatttcc tgtaatttca
    26041 ataaatattg aaaaatatgt aagtttgtgt tgttaaacac aaaatcatga aagtagtcat
    26101 cgttggtgca ggattcggag gcctccggtt agcccgtaaa ctcaataata aagccggttt
    26161 cgaggtagtc ctggtggata agtttaatta tcaccagttt cagccgctct tttaccaggt
    26221 ggcaactgcc ggacttgatg ccagtaatat ttcttttcca cttaggaaag tgtttcaaaa
    26281 aagcaaaaat gtgaggataa ggctggccga tctgaaaagt gttgtcccag atgaaaataa
    26341 aatcatcact gatatcgaag aaataaatta tgatgtactg gtactggcta ccggtgccga
    26401 tacaaatttc ttcggtaatg cagagcttgc cgcacatgct ttcccgatga agtcaacagt
    26461 agaggcttta cagatacggc accggctact tcatttattt gaagaagcac atacaatcac
    26521 tgatgaggat gaattacaac gggcaatgaa tatagtagtg gtaggtggcg ggccaacagg
    26581 agtggaggtg tcaggggcat tggcagagat gaaaaaatat gttttgccca aagattatcc
    26641 ggaattggat ttcacgaaaa tgaatatata cctcctggaa ggaactggta agacactggc
    26701 cagcatgagt cagaaatcat cagaaaaatc gcaacaatac ctggaaaagc tgggtgtaaa
    26761 tgtgctaacg gcaacgctgg taaaaaacta tgatggaaag aatgtggaat tgcaggatgg
    26821 taaccggata ccagccgcaa ctgtgatatg ggcagccggt attaaaggta atattccgaa
    26881 tgggattgat cattcattaa ttgccagagg gaaccgaata aaggtagaca ggtattgcaa
    26941 ggttcagggc ctgcaaaata tttatgcatt gggagatctt gcttatatgg aaacacccgg
    27001 gtttccccat gctcatcccc aggttgcgcc ggcggctatt caacaggcag atatgctggc
    27061 tcataatctt ttattgatag aaaaaaaggc aaatactgac aggttgtatg ctttcgagta
    27121 ttatgataaa ggtgctatgg caacagtagg cagaaacctt gctgtggtag atgtaccgaa
    27181 acccaaattg cattttagtg gcttttttgc ctggctgatc tggatgggac ttcacctgat
    27241 gctcatcctg ggagtgaaga acaggttctt tgtatttatg aactggctgt ataattatgt
    27301 tacgtatgac cagaatcttc ggcttatttt ccgagaattc aacagggaag aatcaataaa
    27361 aaagaatttt aagatatgaa atgggtcgtt ccaaactggc ttatcatttc cggggcaata
    27421 gggcaaatat tcactgcgct tatttatccg tacatccgtc atgtggtatt cgactggtat
    27481 aatgatatca aaaaattaaa gccattgaac caggaaatag ccaggactta tggcaggtat
    27541 atacaaggac ttaatttttc tttcggactt atttcgatgc tgtttaccga agacttaaaa
    27601 aaccagaccg gattggctat tgcacttaca ggtctcattg cagcgtattg gattggtaaa
    27661 gtgatcactc aatttgcgta ttatcccatg tatgagattc ccagaaaatt gatatttaaa
    27721 attggagaag tgggaatgaa tatattgttt atatcgtttg ccgttatatt ttcctggtta
    27781 ttcatttata atttgtcagg ttatttaaaa gggtaaattt tcttacagtt ctttgaggat
    27841 ctttcttgcc cgggctctga atgctgctga ttcatattcc catcggtcat caatgatcgt
    27901 ttttaattcc tgttttattt cagggtattg tttagacaga ttatgaagaa tagtaaggga
    27961 aaaagctttg gcagctgctt tttcatccgg agatgagatg taatcaaaac aggtgttcat
    28021 gatgttacca cgaaaccgtt caggaatggc tatttcctgc aaaagccgga cagtattccg
    28081 tttaacagcg tcatgaagct ttggtttctt aagattcttc agtagcctgg ctaagtgttt
    28141 atgaataaat accggatgtg ctatcactgc ataactgagc ggccaggaag cccgctgcgc
    28201 cataagaggg tcatcatgaa ggaataattc aaaaagggaa tcgaaccgtt gctggttgct
    28261 gccgatccat ttcacaattt tggtacagtt ggctttggaa tgttcagcca gtatcgtttc
    28321 ccggagattc ataattattt tggtacaaaa cggttaccag taagtatcca ttcgccgttg
    28381 gtcagctcat tactttttac tttaataaca tggctgacaa tttttacgcc gcgggaagta
    28441 ttgggaattt taatcgtttt tgtgctggcc ggttcaatat tatacaacgt ataatagtca
    28501 gttctgtatt ccttatcatc tgccagtaaa atactgactt caattataat tttttgaaaa
    28561 ctgaaattca atgaattttg tatcgtaacc atggccccgg aaataccttc cgtatttact
    28621 gtagtttccg ggatgattga cctggaaacg aggaactggt caatattggc aagaatgata
    28681 ttgggatccg tagttggttg taaactggta ctgtcgggga gagtaaccga actggatttg
    28741 ttattagaat ttaaatcagt atttaatttg ctggtttcat ttttttcatt gggtacttca
    28801 tcagtttcag cttttgttgc tattccgttg caaccggcaa ttataacaag caaggaaatt
    28861 agtaatagat ttctgtgaat ttgcatagca ggtgatttgt atgcaagcta ctcagacctt
    28921 taattcgggc caatacccat ttcccgtaag ataaagggta aaaatattcc tataattatg
    28981 cgttatgtta cttaggtcgc cgttgctata agttttagca aatagcaacg gattgttcta
    29041 taaaataaaa aagccaagtt ctcaccggta tgagccctta gcttctttga gagacgaccc
    29101 acttgttaac ctttcgtgtt tcgtcaatca ttttgctctt cggaagtgaa gaggaggccg
    29161 aactttgcgg agttcggctt ttttatttta taggacaacc tatttacaca taacggccat
    29221 tataagaaaa ccaccttcca ctgcgaatat acggattttt tcaatcctac taaacaagtt
    29281 agtatctttt gaaaaaatcc gtatattcgc agtggccaca agccatagtt ctttttctta
    29341 taatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaaaa aatccgtata
    29461 ttcgcagtgg ccacaagcca tagttctttt tcttataata tatattatgt taaataaatt
    29521 taacataaga aaagccacac aatggtgact tttctacatt tcaatatctc ctcttttcta
    29581 tcttattaat gatctctctt tttttcctta attgagttta tcgcatcata tgcgtcggtc
    29641 catttttttt cttccgccga gtacttggcc tgatctgcag gtttcccttt tgccattgcc
    29701 cttttgaaag caaaaatatc tgccaggtag cttacagcct ttttatactg ttgtttatcc
    29761 ctcatttcca gctcattttc ctgtttgact ttattggcaa aaatggcagc tactttttca
    29821 tatggttctc tggcttgttc cagtgtctct ccatattctt tatccagggc atctcttttg
    29881 gcaatatctt ctttagaaga catagctccc ggtttggttc gggctttcat gtcttttgaa
    29941 tgagcttctc ttctatcatt tactttggat gctttattga taaaatggtc acccatatat
    30001 aaatatggaa tttcagctgt ttcaggtttt gcttttgccg atttttcaaa agcttctttc
    30061 attttctttt ctaattcaac tgcatttgga agaggatcct gcggtgcatc ttctttagga
    30121 tttaatgtgt cgtatattat ttcacccaac acctggttcg ctttgtagtt atccggatct
    30181 gtggctaaaa cttcttccag cgatttcaat ttgtcattaa aattggtcat caaacctact
    30241 gcaaaatcta ttttatcata gttaaagtat tcgcttttag ggaataattc tttacccagc
    30301 gctttgtact tttcaaatgc agacatgtct tttttcgcaa atgaataact aacaagaaag
    30361 cgatacactc cttcaaaacc atcaccgcca atttttgcat ctgctaaacg ggtataatag
    30421 ctggcagcat cttctttatt accggaattt tcagcagtaa tagccgccag gatgagcaca
    30481 tttgtatcta caggtgaaga gagaatcttt tttaagatga gcagatcaga gtattctatt
    30541 gcttttttta atttaacaag gcttgtttgc cattttttgg cactgtactc attgtaccct
    30601 gctgtgtaat aacttgagta caggttgatg gggccatttt gataagttgg gtcacttatt
    30661 agggccattg taggatccat ctccttgtat ttgctaaatg ctgcatctgc ttcagcagta
    30721 agttgctctg attccggagt attttttttt gcatctgaca tggccagtcc tgcataaata
    30781 gcggttttca taatataagc ttccggcttg cctgcaaatt ttgcattggt aaaggcttta
    30841 tcaaaatctg cttttgcctt atccagctga ttcagcatca taatggtctt aatattgtca
    30901 tagttctgcg cagtggcgaa aaaggctgac aagcttaacg ttaatagaag gaataaactt
    30961 ttcatctgtt tatttttagt tttaaatgta tgttcaaata ttagttgcca ataaaactat
    31021 tgtatacagg acacctgtta acccggtagt tttagaagca catttcatat tttaatctgt
    31081 tgcactttca gtagatggat tttcacctgt tccggttgcg ttaccctctt caccgttctg
    31141 aatttcttca ctggttgaat cgccatggcc attgtgttcc tggtcatcca ggtttgttat
    31201 ggcagcaatt tcatcacctt catccagcct gatcaatttt acaccctgtg tggcccttcc
    31261 ctgttcgctg atgccggtaa ctggcatccg gatggtgacg ccgcttttac aggtgatcat
    31321 gatatcttcc gtttctgcca catcaaggat acccaccaat gagcctgttt tatcggttac
    31381 attaattgtt ttcacacctt ttcctcccct gttagtaaag cggtattcgt caaccggggt
    31441 ccttttgcca aacccctttt cactaactac cagcacggtt ttggatgcat cagtttctgg
    31501 atttacacaa atcattccta ctacttcatc gttgttgtct tcaacttcta ttcctgccac
    31561 accgatggca ccgcggccgg tggctcttac tttctcttca gagaaccgga tggcccttcc
    31621 gctttt
//
LOCUS       DAORTI010000027        30647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1039_length_30647_cov_8.635460, whole genome shotgun sequence.
ACCESSION   DAORTI010000027 DAORTI010000000
VERSION     DAORTI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 30647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30647
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_30647_cov_8.635460"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="purT"
                     /locus_tag="PLO70_05575"
     CDS             complement(<1..953)
                     /gene="purT"
                     /locus_tag="PLO70_05575"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HQZ73962.1"
                     /translation="MHKKILLLGSGELGKELVIALKRMGQTVIAVDNYAGAPAMQVAD
                     GFEVIDMLNGDQLDSVITKHQPDLVVPEIEAIRTERFYAYEQQDIQVVPSAKAANFTM
                     NRKLIRDLASKELGLRTAKYFYATTYEAFTRAVNDIGMPCVVKPLMSSSGKGQSVLKK
                     VEDQQTVWDYAINNSRGDIKEVIIEEFISFHTEITLLTVTQKNGVTLFCPPIGHRQER
                     GDYQESWQPVAISESDYADACAMAKKVTTALTGAGLWGVEFFLTDEGVYFSELSPRPH
                     DTGMVTLAGTQNLSEFELHARTILGLPIPEIKLERAGASAVV"
     gene            1058..1888
                     /locus_tag="PLO70_05580"
     CDS             1058..1888
                     /locus_tag="PLO70_05580"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQZ73963.1"
                     /translation="MTDYERIEKAIHYLQENFTRQPDLDEVAKQVHVSPYHFQRMFKQ
                     WAGVSPKKFLQYISAEHAKTLLKQNMSLSDVSYETGLSGTSRLHDLFISIEGMTPGEY
                     KNGGEQLHINYSFAESPFGNIIVASTTKGICHLAFADNEKLALEELQRLFPKASFRQV
                     VDTIQQNALFIFTQDWKNIASIKLHLKGTAFQLKVWEALLKIPLGSLATYSSIAAATG
                     NENASRAVGTAVGDNPVAFLIPCHRVIKSTGSIGQYHWGSNRKTAIIGWESAKIFAES
                     "
     gene            1949..2671
                     /locus_tag="PLO70_05585"
     CDS             1949..2671
                     /locus_tag="PLO70_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ73964.1"
                     /translation="MKTFILIHGSWHSAWNWHKVVPLVEKAGHKAIAIDLPGMGRDKT
                     YISKVKMKTTVEKICELIDSIEGKVILVGHSKNGIMISQAAEYRPHKIEKLIYLAAYL
                     VPNGKTQREYSLLDTGGMLKPYVTPYPELNAHTLQPAIYKEGLYHDCDEDITELAKVL
                     LSHEPVESGITPLQLTEENFGSVPRYYIECTEDRAVTPFIQQKMYEETPCKKIYRMAT
                     SHSPFFSQPEALTEIFCEIAAE"
     gene            2771..3373
                     /locus_tag="PLO70_05590"
     CDS             2771..3373
                     /locus_tag="PLO70_05590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQZ73965.1"
                     /translation="MKFILPVLLLLSITSCSVSKNPLRKQVKLLQKGINKDDTSYVYA
                     LPYEEGKTFRVIQGYFSRFTHKERAALDFNMKRGTKISAAREGVVTRVKEDGTKGGLK
                     KKYRPDGNNIVIQHPDGSRAGYWHLQHNGALVNVGDTVKKGQVIGLSGKTGYAMTPHL
                     HFLVWRSGGGQWQQVPTRFQTSKGIKYLKGWRKYHNSKPE"
     gene            3380..4024
                     /gene="pnuC"
                     /locus_tag="PLO70_05595"
     CDS             3380..4024
                     /gene="pnuC"
                     /locus_tag="PLO70_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HQZ73966.1"
                     /translation="MIKTILSLQEIYQQFIEGMKNTGWLEYIAVFAGIASVWFSRKEN
                     ILVYPVGLINTIIYIYLSFRFHLPGEASVNFYYTVMSFYGWYCWLQKDTRQENILHIS
                     YSNKQMWLNQVLFFLFFYFTIYFSLVYLKDKFFEGAIPWADAFASATAFTGMWLMTRK
                     KVESWYWWIATNIASIPLYFVKGLVFTSVYYLVLLVMSVFGLTEWIKRAKQQHA"
     gene            4017..4574
                     /locus_tag="PLO70_05600"
     CDS             4017..4574
                     /locus_tag="PLO70_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQZ73967.1"
                     /translation="MLKKIVILGPESTGKSTLCEQLAQHYETMWCPEFAREYLLTNGT
                     NYEYEDLLTIARGQLALEDEYSSTLERNSLPMMEEGGHLPLFIDTDMYVMKVWSEFVF
                     GKCHRFILDQIGERKYDLYLLCNTDLPWVEDELREYPELHTRKKLYSIYKDIMVNQPV
                     PWVDISGDDDARLRKGIAAIDTLLG"
     gene            4596..6263
                     /locus_tag="PLO70_05605"
     CDS             4596..6263
                     /locus_tag="PLO70_05605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQZ73968.1"
                     /translation="MQNIFQKHHRLLFYGTWLLLGLIQSGLTELQDDEAYYWVYGQYL
                     DWGYFDHPPMTGLLIKMGYAIFPNELGVRLFPLLLNIFSLLIIEKLIDKKNPLLFYGI
                     SLSIAVIQITGFVAVPDIPLIFFTALFFLFYKRFTGNYSLLNTFLLGLSVAFLFYSKY
                     HAVLIVFFTLFSNIKLFTKYQTYLAGIIALLLFAPHLWWQYQHDWVSFRYHLFESNVN
                     AYKPSFTLDYIIGQLLLPGPVAGFILLPAAILYKTKNDTEKALRFTMIGIYVFFLASS
                     FRGKVEGNWTSPALVSLMVLAHQFLYEKLQWQKILYKLLPVTLILVVFARIVMIADLL
                     PVKAIKDRYHAWKVWPQEMKEKTKGLPIVFSNSYQRASKYWFYSGQPTYSLNLYKERK
                     NNYNFWPLEDSLLGKPVYYLDKYDLWRFKDSLQTPIGWVGYKYDSSFASFAKIRIDVS
                     PQKIRVKEKEESRLICSFRIPQQYEEFIQQHKPQNDTVRIGIFSTKGWIRDIYTTITL
                     QQLVRDKTLPLTINPGLRSGKYYLRFAINSGYRNPTHNSDKIELVID"
     gene            complement(6260..7015)
                     /gene="mtgA"
                     /locus_tag="PLO70_05610"
     CDS             complement(6260..7015)
                     /gene="mtgA"
                     /locus_tag="PLO70_05610"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQZ73969.1"
                     /translation="MATSKENKPGGSVSFLSKCWKWVKRIFIWLFIIQLFLIILFKWV
                     NPPITITQLVSWVSGHGLKRDYINTGNISPHAKLAVIASEDQLFPDHSGFDWKSIKKA
                     MKYNERKPGRIHGASTISQQVAKNVFLWQGRSWIRKGLETYFTFMIEQVWGKKRILEV
                     YLNVIETGDGLFGIEAAAQEYFNKPAKNLTKQEAAMIAACLPNPKRYKVKPPSSYIGY
                     RSRVIVQQMNNLQTDPDIQRVIGTLPAPDKKKN"
     gene            complement(7002..7631)
                     /locus_tag="PLO70_05615"
     CDS             complement(7002..7631)
                     /locus_tag="PLO70_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQZ73970.1"
                     /translation="MVEALLKGITIGLLLSIAVGPILFSVIKQSLNNGHQGGMAFAFG
                     VSASDITLVLVANVFTELFRSLRLYETQIGIAGSVLLILMGIFFLYFKKVKVNEDGKL
                     VFKQRKRDLLKISLSGYFMNLMNPGVIIFWITISTSLINFSNKHKIVIYTAALAIVLL
                     ADLVKVFLAGKIRNRLTPHNIHILNRISGIILIIFGLVLIGGLIFYGNQ"
     gene            complement(7766..9298)
                     /locus_tag="PLO70_05620"
     CDS             complement(7766..9298)
                     /locus_tag="PLO70_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQZ73971.1"
                     /translation="MATILDIKLPKAIAAALRIPPNDPRRQQLRVLKKLLKKARFTEF
                     GQRYHFDEALLSKHPGKKFQELVPVHDYNKIYEEWWKKTLEGVPDVTWPGKIKYYALS
                     SGTSEAASKYIPVTKDLLRSNTINYLRQLISLIRYEDANKRAMTKGFLMLGGATDLKK
                     GKAGWYAGDVSGILAKKRPFWFQNFYKPGGRIAAMSDWNQKLNEIVEHAKEWDIGYIV
                     GVPAWCQMCMEMVIEHYKLNNIHEIWPNFSFFVHGGVAFEPYKKGFEKLLGKPIVYIE
                     NYLSSEGFIGFKTREHARGMQLILNNNIFFEFVPFDDKNFDHDGKIVDNPEAKMIHEV
                     EEGKEYALLMSTNAGTWRYLIGDTIKFVDLEKSEVCITGRTKHFLSLTGEHLSVDNMN
                     KAIELVSEELNVSIPEYTVVGFPYENFFAHRWYVATDDKVDTDKLRKRIDEQLRVLND
                     DYATERDSALKEVFLEVLPEEKFMKFLELKGKLGSQHKFPRVMKGKMLEEWERFLATG
                     SL"
     gene            complement(9384..11327)
                     /locus_tag="PLO70_05625"
     CDS             complement(9384..11327)
                     /locus_tag="PLO70_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ73972.1"
                     /translation="MQLSVIIVNYNVKHFLEQCLCSVLKAIEGMQAEVIVIDNCSADN
                     SINYLTPKFPVVRFIANTENIGFAKACNQGLKQSSGKYILFLNPDTIVPEDCFQKCIS
                     FLDQQADAGALGIKMLDGSGRFLKESKRAFPSPLTSLYKLFGLAKLFPRSKTFSIYHL
                     GHLDENNNQEVDVLAGAFMMIKKEVIEKVGSFDETFFMYGEDVDLSYRIQKAGYKNLY
                     FAESSIIHFKGESTKKGSLNYVRMFYNAMSIFVRKHYWGSRAGLFNLLIHSAIWFRAA
                     LTAVVSFIRRIGLPLIDALLILFSFWLIKNIWNQYVRTDIEYENKLLWIAIPAYTVFY
                     LVTAYYAGLYDKWYKWGELIRSSAIATIVLLAAYSLLPEQYRFSRAIVLFGALLAFAL
                     ISFLRWILVQTNVVNSEKEKEDKAATVIAGSVDEYENTIRLLKEAGLHQRVLGRLAVT
                     TNDTTAIGSLEKINQLSQMLPFREIIFCQGNLSFAGIIQTIQQLPPGIIAKIHAVNSG
                     SIVGSDSKDSSGEAVSTENGYKLADPYNRRLKRLIDVSLAGFGIITFPVQLFIVKKPV
                     NFFMNCFMVLLAKKTWVGYAVPETQLPSLHTPVIACNGVPGTSLQQLSPESLQMMDYW
                     YARDYEPSTDLKLIWRTYRRLGG"
     gene            11446..12225
                     /locus_tag="PLO70_05630"
     CDS             11446..12225
                     /locus_tag="PLO70_05630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQZ73973.1"
                     /translation="MLKTTLIEAAKAGAAEIVRFFNSDFKISNKEGVNNLVTEADHAA
                     EKAILSVIKSHFPEHQILAEETGEIIQDSGYKWIIDPIDGTVNFAHGIPLNCVSIGIE
                     HAGEIVMGAVYNPHLNEFYFAEKGKGATLNDKPIHVSRETQTIKACLVTGFPYTYINM
                     PNGPLEIFERFIRKGVPVRRLGSAAIDLCWVAAGRFDGFYEHKLEAWDSAAGYLMVEE
                     AGGKVTDFTGGKFSVYQHRVLATNGKIHEEMLDIINNKKEL"
     gene            12222..13256
                     /gene="thiL"
                     /locus_tag="PLO70_05635"
     CDS             12222..13256
                     /gene="thiL"
                     /locus_tag="PLO70_05635"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQZ73974.1"
                     /translation="MSTERTEISSLGEFGLIEHLTKNIELQNASSILGVGDDAAVIDH
                     FGKQTVITTDLLVEGVHFDLMYTPLKHLGYKSVIVNLSDIYAMNAIPTQIILSIGFSN
                     RFSLEALTEFYDGVYAACEKYGVDLVGGDTTSSQKGFIISVTAIGEVAPDKYVKRSTA
                     QKGDLLCVSGDLGAAYVGLLFLEREKKIFMESPGVQPDLEGETYVIGKLLKPEARKDI
                     VEFFAAKEITPTSMMDISDGLSSEILHICKDSTLGCVLYEEKIPVSEEMKKAAFKFEI
                     DPTACALSGGEDYELLFTVSQSDYDKLVLNEQISVVGYMTEPELGAHIITKGGGKHAI
                     TAQGWNHMKH"
     gene            13279..14787
                     /locus_tag="PLO70_05640"
     CDS             13279..14787
                     /locus_tag="PLO70_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQZ73975.1"
                     /translation="MGRLQILLALLIALTGCGVSKSAFSPVKKYSPQQLERDYSLYQH
                     ILEQHHPSLYWYTSKDSMDLYFMYGRQQLKDSMTEPEFRKLLSYVTAKINCGHTSIRN
                     SKAWSKYSDTVRLGKMFPLSMKVWNDTMLVTANLNRKDSILKRGTMITRINGKDIPTL
                     TDTLFEYISTDGYNRTHKYQSLSNRGYFGSLYTLLYGPSEKYTLDYIDSTGQQKSITI
                     PIYNPAADTAGRMMVRPPARIPRPSKRERRRQQISTIRLLRIDTVNHSAMMTLSTFGR
                     GYGLKKFFRQSFRALQQKNITHLIVDVRGNGGGSVNNSTQLTRYIARQPFKISDSLFA
                     IRKRSNYEQYIQHHFWNKLFITLFTRRRNDGNYHFGYFERHYFRPKKNNHYNGKVYIV
                     TGGNSFSATTLFTGALVKQNNVIVVGEETGGGAYGNSAWLIPYATLPETGVRFRLPLF
                     RLVIDKTVPKNGRGVQPEITSFPSIEAIRRNADFKIETVMELIQKDKEEKKN"
     gene            complement(14825..15628)
                     /locus_tag="PLO70_05645"
     CDS             complement(14825..15628)
                     /locus_tag="PLO70_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQZ73976.1"
                     /translation="MSALLITTIQTNLHWEDKIANLQMLEEKISSISIKTEVVVLPEM
                     FSTGFSMKPEELAETMEGETVQWMKRTAAAKKIVLTGSLIIKEANNYYNRLIWMLPNG
                     QYGVYDKRHRFAFAGEDNHYTAGTKRLIASVKGWKINLQVCYDLRFPVWARQQSQPEG
                     PEYDVLIYVANWPERRSLAWKTLLQARAIENQCYVIGVNRVGNDGNNIYHSGDSMIID
                     PMGEVLYTKKDEEDVFTIQLEKKQLDNIREKLPFLKDADGFMIMKDEEE"
     gene            complement(15730..16626)
                     /locus_tag="PLO70_05650"
     CDS             complement(15730..16626)
                     /locus_tag="PLO70_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQZ73977.1"
                     /translation="MKRYILFAVFAGVVTTVNAQQLQTSSMYDMQGVLHNPSTAGVYQ
                     KGMVGATYRSQWSGISGSPKTTTVFGSFELPEQKIGLGGYLFSDKTGPTSRTGIALQF
                     AKHIPMANGGKFSLGIEAKGLQYSLDRAKLSETLGSDPALGTSDNRFKFDAGFGVSYT
                     GKKLQLGASVAQLVQSKLGYYTGTVSPSEEARLYRHYYFHGSYKWNVDEATSVTPNFL
                     FIYLPNAPLEFQGGIRVEHSEVFWWGLALRAKQSFILSAGVNLNKKFTVGYSFDIYKT
                     PISVFDAGSNAHEVLLRYNIFK"
     gene            complement(16648..20016)
                     /locus_tag="PLO70_05655"
     CDS             complement(16648..20016)
                     /locus_tag="PLO70_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73978.1"
                     /translation="MNKNCLGKLHYYLLIILFSFSSMLVIGQGYDTTKWKFSNPKQFG
                     FTLQDVDFFDNNNVIAVGSDGGIARSRDGGSNWTYGPFTFVNPANGFLTKPSFSDVHF
                     VSSTIAYAVGNLGCMAKTTDGGLTWTLINTPLSSNARNINAVWFTSPTTGYIGGQWNT
                     PDSIPKLYVTNNGGATWDSMAAPVSNGTTKVGFINNATYPSIDFPITAKAKEIQRIEF
                     INNNIGYISGGGASNFPGLGIPNITNSTTCALTGQQTTGSHNASLLWKFDNGTLTDFS
                     ISKERLGYTGFPATINCTSKFGTVSQVNQQFRALNIINDTLIVMMSFNNNVVVRVRTG
                     KNDSTLNVNRPGVYEKGKFEILNTGPAGPPPGYPPVPAVQVLLASNPYQMRRTSNGTL
                     IANGNFARMWTSVDTGRNWVESRSLPPGQNYSNNGVWALDIAPNGKFLSMGSLGVVAD
                     SIPGGPGWSSNYVTVPLSAGFTKMEFADCNNGVAAGSSTINVTADGGKTWWDRTRPDF
                     ASSFYSINGFAYRNSNPAQAYFAVSNGSIYYTTDLNVAPPAVPTLDPVYADGLMQMND
                     LATVGNDSVWAIGYTSFSVPSASRTSRVYRSFNGGATWSVYSGFSVGSLAQNLTEIEF
                     PTRLIGYAAGNRDTIYKTTDAGVTWSKLPLPTPGVTPQITYKDMIALDANTVFVIGNG
                     FPRKVIFRTTDGGATWTDVSGNIGTLGVGNLNAIVMHDINNGYVMTPGLMCKTTDGGA
                     TWTLEVPPTSCLFETAAFAPKTVPAAIPFANRRLFVTGVNVSGAPIMEFGNPANIAVN
                     STENVVNASCTNLTGGSITVNATGAISPYLYSINGGAFQSSNTFTGLTQGPKTITIKD
                     SYCGLLTKTINVGFNDNLVLTTNNDTLVCAGAPVPMLASTNGTGTTYAWSPAGGLSAT
                     NISNPVATVNNSAAFTVTASLNGCVRTKTVNVNIKPNPIISAGPDKTIVDGDEVTLEG
                     SGTSTPSSIAWTPNNTLTGANTYTPVAKPNTTTTYTLTVRDNNNCTSTDNMVVNVIPY
                     CLKVMNAFTPNGDGQNDVWIVTNNGGTCTRQVFATVFNRYGNIVFKDDNYQNKWDGTY
                     KGKPVADGTYYYMITYRLINGASITLKGDVTILR"
     gene            20146..>23639
                     /locus_tag="PLO70_05660"
     CDS             20146..>23639
                     /locus_tag="PLO70_05660"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ73979.1"
                     /translation="MRQKLSIILVISLFFGSLSNAQDKSNRGKEFWLGYGFHWLFLNP
                     DITPVNSQELALYISSEQAATVTVSVNGTAWTQTVNIPANTVDATILIPKTGPNDARL
                     LTDGFSNKGIHIVSDVPVAVYAHVYVNQGSGATMLMPVETYGYSYSSVNYRQNTSGSS
                     LPNISPTTQNGPDWYSWFYVVASEDNTRLEITPSDTTKNGWLPGQTYTVNLNKGEIYS
                     VYGKMVPGNSQAWAASKDMTGSKVIAVPGSDGNCHPFALFSGSSGIRICRGDGGEYMQ
                     QQMFPSQAWGTRYLTYHTINNTNTDILETNRNYYRVCVSDPTAVVRRNGIPLTGLVNN
                     FYYELMDSTGGDYIESDKPVLVSQYTVNDNQCWNFPTTTPSPPSNGDPEMFYLSPIEQ
                     GQKSVRFFASRQSPAISYVYTNIHVPTIAVGSLRVDGNVVPASQIVVHPNNPAYSVAL
                     ARFTGPAAQHTITCDSAFTATVYGLGNYESYGYNVGTLINNLNHYSEIRNTFNTTGAV
                     DTFTCPKSPVRLFVKLGYPANSIHWKLSQVTGMSPNADSIISNPVPVRTEIINGRTYY
                     VYTLQQDFIFAHPGTYYIPVSFTATVIENCSQTENATVKVVVKPGPVADFTFPSPGCL
                     TDSIRFTGASVPGTFNLVNYNWLFDDNSTANTVNAMKLFTMAGNQNVRYTIFADNGCI
                     GDTIKTVAIQESPVAAFSISGNSCQRDSVRITDASAIPAGTITSWQWNFGDGNTATLT
                     NNNPFYHQYTTPGNYTISLITVAANGCKSDTAFVPVTILPKPVAAFTTGGNICLGDSV
                     RFIDASTAPVGTITSWQWNFGDGNNATYTNNNPFYHPYTTAGNYPVSLIVTGSNGCIS
                     DTFRLTVNVSSKPTASFTFIGKPCIDSVQTFTSSVPTGGSNPPTWHWNFGDGQTFNST
                     TVNTVTHSYTATLSNIVVKHWVSYSAGCSSDTSFSTIPLINPNPIASFAVTGDTLCER
                     KPITLTSSVTGVSSWNWDMGNGTSNAVPPFNHSYTAANTYTISLFVRTAAGCGSLPAT
                     QSVTINPTPAVNAGPDKFIRIGTSTTLDATINNAANYNYLWTPSLYLNATNILNPVST
                     PDNLVTYTIQAIDKLNNCIGTDDVVVTPVSDIFIPSGFTPNNDGKNDKWVIPGLALYP
                     EARVTVFNRAGQIIFDGKG"
     assembly_gap    23640..23739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23740..26382
                     /locus_tag="PLO70_05665"
     CDS             <23740..26382
                     /locus_tag="PLO70_05665"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ73980.1"
                     /translation="HNALFANASPPNSQNHTLYLSAEQAATVTVSVNGTSWSQTVNIP
                     ANTVDFSIIIPKSGADDSRILSEGLFNKGIHIVSDVPIVVYSHQYNLFSSAATMLMPV
                     ETYGYTYYSLNYSQVSNYPDSYSWFYVIAPENNTRVLITPSDSTQGGWLPGQTYTVNL
                     NKGQLYNVFGKRADTYTGKDMSGSKIVSVAGADGNCHPVAVFSGSSRVVICNGNGGEI
                     LQQQIFPANAWGTRYITYHTVQSAMGDITSPLLNIYRVAVRNPANIVKRNGVPLTGLI
                     NNFYYEFSSTSGDYIEADQPILVSQYTVSANECTGSSTGPYGDPEMFYLSPIEQGVKN
                     SVFYSTRREAIDLNFINVIIPQGGLSSLRIDGTAVLPAEYITHPVNNNYAVVVKRLWG
                     PAAQHSVNSDSTFIATVYGVGTLESYGYNMGTYVNNLNAYSSIKNSLNQTNQADTFTC
                     LKTPFRITIKLAYKASSIHWKLSQVPGLNPNTDSIIVNPIPIDSSFINGRKYYTYTLQ
                     QDFTFATAGTYYIPVTYMAPDIDACNQTESTTVKVLVKPGPRADFTFNAPACLADSIR
                     FTGTAVPGSFNLINYNWLFDDNSTANTINAVKLFTNTGSQNIRYRIYADNGCLGDTTK
                     TITISESPTASFSISGAPFCEGKPVTITSSASGISSWNWELGNTTSTAVPPFTHSFST
                     GTYSVKLVAKTTSGCLSDTAEQNITIYPTPVVNAGADKTIRAGSSVTLDAAVTPAGDH
                     NFSWSPSLYLDAPAILNPVSAPLVSTSYTIRVVDRQSGCSGEDDVTVTVISELFIPNA
                     FTPNNDGRNDKWIIPGLALYPEAVVSVFNRGGRKIFESKGYTNNPWNGTFKGISQPND
                     VYVYLIELRDEAKKIIKGTVAIIR"
     gene            26472..27080
                     /locus_tag="PLO70_05670"
     CDS             26472..27080
                     /locus_tag="PLO70_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ73981.1"
                     /translation="MPPKILIADDHSMIRKGLKLHIQLTLGYNDIEEVATCNDLMKEL
                     VKKKYTHLVLDIILSDGSTLEVIPNIRRVYPDLHILVFSMQPAEIYGEALKQYGINYY
                     LSKSVGEEEMIQVLQKFLQNETPIRRNDIHHHDNPFKTLAPRELEILHYVLKGIGTKE
                     IGETLNLKMNTVSTIKNRIYEKTNAGNIKELMELATLYNVNY"
     gene            27115..30174
                     /locus_tag="PLO70_05675"
     CDS             27115..30174
                     /locus_tag="PLO70_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ73982.1"
                     /translation="MQRFLILLYFILALANPAMSQTAGNSHLRQYTTDNGLPSNGIKG
                     LQWDEKTGFLWIATEAGIVRFNGVDFRSYTKENMPSITTERMLFMVRNRKEHIYISDM
                     AGNIFWINQNKPVLWKKGAENVNPYYANYYLLSVSDTFFERHTGVANNTRFSAVTDKI
                     VGLSDTACLLLNNGTLFYHSITMPAPVALPFERGSFKSFFKINDQYFLINNKKEALLL
                     NTSDFTVSPVAVTDINGSRLLFETGNNPVFWEPGMNNPVLVEEENAWLFTFRDNKITA
                     TLLFTGIPSDALIRTLEYSDKNKTLFIGTDSKGLIIINQTSVTTKKRTDSVSKNRNSY
                     YSQIELADGNVLTNEGDIIGDTPAAENTIPVKGKFAYYVSSTGDSLLWYSQYTPSLGY
                     SCLHRYNKVTGEIKVFSKIKPDQVVTQSGTNYYLASNTGIGVLDADSIRLLHTYAGRN
                     AGIIAFAIAEIQPGVIAVATCSGLLRYTIVTGRLDTIFSKENICVRSIWKYKDYVFFG
                     TYGSGYYIYKNGVIKQMPLDKNKYLLYAHCFVPDDNGYCWISTNRGLFKSSLEELINV
                     FENKKTSVYYHYFGKKDGMEMTELNGGCTPCALRLKNNIISFPSMDGLLWVNPDKAIP
                     VLPEGDIFIDEILIDNEIKDPGYLSQKSLPAKTGEIIIRLAFSAWCNKENIYLEYQLN
                     DTLNWKPVVFENGSEIRFNNLPSGDYKLRIRKLNGFGFNNYTYKTVDFTITTPWHQQW
                     WFFVLASLLVLGMLSFYFRFRTRQYEIRQRKLEQQVAEKTKELKEQNEVLEKNNSIKT
                     RLISIISHDIVTPLKFLTVAGKNLIEKRKMMPEELQQETLQEITNTSQELQLLSTNIL
                     NWIKYQNENRRLAKETFNLQEMVNQILGILQSLARQKNITIDNQVEKELEIYQYYEPL
                     KILIYNLLTNAINFTEKGTITITAMRQNDQVSIAVKDEGIGMSPEQIQRLMAEDVVIT
                     SANVDNRKGHGLGYLIIKDLLKTMGARLHIESKKGQGTTVSISMFAL"
     gene            complement(30169..30642)
                     /locus_tag="PLO70_05680"
     CDS             complement(30169..30642)
                     /locus_tag="PLO70_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQZ73983.1"
                     /translation="MYKKHLTKEQALQKLKHYCAYQERCHSEVKEKLFNLGVWKKEHD
                     EIISTLIEENYLNEERFAIAYAGGKWRVKHWGRVKIKYELKQKQVSEYCIKKALQQIS
                     QEEYLAVLKKLADEKYASLKAEQYLVRKKKTMDYLLNKGFEMDLVRGLVGKEKPL"
BASE COUNT         8887 a         6575 c         6217 g         8868 t
ORIGIN      
        1 actactgcac tggcaccggc tctttccaat ttaatttcag gaatgggtaa tcctaaaatc
       61 gttcttgcat gcaattcaaa ctcagaaaga ttttgtgtgc ctgccaatgt caccatacct
      121 gtatcatgtg gcctgggact tagttctgag aaatatactc cttcatctgt cagaaaaaat
      181 tctacgcccc atagtccggc ccctgtcaat gcagtagtta ctttctttgc catagcacag
      241 gcatctgcat aatcgctttc actgatagct acgggttgcc agctttcctg gtaatcacct
      301 ctttcctgcc ggtgtcctat cggcggacag aacaaggtta ctccgttttt ctgtgtaaca
      361 gttagtagtg ttatctctgt atgaaaggaa ataaattctt caatgatcac ttctttaata
      421 tcgcctcttg aattattgat agcatagtcc cacaccgttt gctggtcttc cactttcttc
      481 aacacacttt gtcctttgcc gctggaggac atcagtggtt taacgacaca gggcatacca
      541 atatcattta ctgccctggt aaacgcttca taggtggtgg cataaaaata tttggccgtc
      601 cgcagtccca gttctttact ggccaggtcc ctgatgagtt tcctgttcat ggtgaagttg
      661 gcagctttgg cactgggcac cacttgtatg tcttgttgtt catacgcata gaaccgttct
      721 gttctgatgg cttcaatttc cggcacaacc aggtcgggtt gatgtttggt tattaccgaa
      781 tctaactggt cgccattgag catatcgatc acttcaaatc catctgccac ctgcatcgcc
      841 ggtgccccgg cataattgtc cacggcgatc accgtttgcc ccattctttt cagggcgatc
      901 actaattctt ttcccagttc accgctgccg aggaggagta tttttttgtg catactgcta
      961 aaaataagtc ataaaaatag tgtagtcccg ttttccgcaa gaaacgggtt gtacccatga
     1021 acagcgtatg caatttttgt gtcgtaaatt taaaacaatg accgactacg aaagaattga
     1081 aaaggctatt cattatctgc aggagaattt tacacggcag cctgacctgg atgaagtggc
     1141 gaaacaagtg catgtaagtc cttatcattt tcagcggatg tttaagcagt gggcaggagt
     1201 cagtccaaaa aaattcctgc agtatatcag tgctgaacat gccaaaacat tgctgaaaca
     1261 aaatatgagc ctgagtgatg taagttatga aacgggactc tccggtacca gccgcctgca
     1321 tgatcttttc atcagcattg aaggaatgac accgggagaa tataaaaatg gcggggaaca
     1381 attacacatt aattacagtt ttgcagaatc cccgttcgga aatattatcg ttgcttccac
     1441 tacaaaaggt atttgtcatt tggcttttgc agataatgaa aagctggcgt tagaagaatt
     1501 acaacgactt ttcccaaaag cttcattcag gcaagtggtg gatacaatac aacaaaatgc
     1561 attgttcatc tttacgcagg actggaaaaa tatcgcttcc ataaaactgc acttaaaagg
     1621 aacagcattt cagctaaagg tgtgggaagc tttattaaaa ataccactgg gtagtttagc
     1681 tacttattct tccattgctg cagctaccgg caatgaaaat gcatcaagag ccgttggcac
     1741 agcagtgggt gataatcctg ttgccttttt aattccctgt caccgggtca tcaaatcaac
     1801 cggctcaatc ggacagtatc actggggcag caaccgcaaa accgcaatca tcgggtggga
     1861 gtctgccaaa atatttgctg aaagctaatt ccgcaagttt cgggttgctg accacccctc
     1921 ttcttttcat ttttacagaa aaaatacaat gaagacattt attttaattc atggttcctg
     1981 gcatagtgcc tggaactggc acaaagtggt gccgttagta gaaaaagcag gccataaggc
     2041 tattgccatc gatctgcccg ggatgggaag agataaaaca tacatcagta aagtgaaaat
     2101 gaaaacaact gttgaaaaga tctgtgagct tattgacagt atagaaggca aagtgatatt
     2161 agttggtcac agtaaaaacg gtatcatgat ctcccaggcg gcagaatacc ggccgcataa
     2221 aattgagaag ctcatttatc ttgctgctta cctcgtacca aatggaaaga cacaaaggga
     2281 gtactccttg ctggatacag gcggaatgct taaaccttat gttacaccct accccgagct
     2341 gaatgcccat acattacaac cggcaattta taaagaaggg ctttatcatg attgtgatga
     2401 ggatataaca gaactggcaa aagtattatt aagccacgaa cctgttgaat cgggtattac
     2461 acctcttcag ctcacagaag aaaattttgg ttctgttccc cggtattata ttgaatgcac
     2521 agaagacagg gcggtaacac cattcattca gcaaaagatg tatgaagaaa ctccctgcaa
     2581 aaaaatttat cgcatggcga ccagccattc gccatttttc agccagccgg aggcgctgac
     2641 cgaaatattt tgtgagattg ctgcagaatg aatactagtt tgcttttatt tgacaaacaa
     2701 ttaaatccaa gaactgtgtt gttacccggg atggacttat ttttatgccc cgatgcaaat
     2761 accagtactt gtgaagttta ttctgcctgt tttattgcta ctgtctatta cgagttgttc
     2821 tgtcagcaag aatcccctgc gcaaacaggt aaagctttta caaaaaggga tcaataaaga
     2881 cgataccagt tatgtgtatg cattgcccta tgaagaagga aaaaccttcc gggtgattca
     2941 gggttacttc agccggttta cccataaaga aagagcggcg cttgatttta atatgaaacg
     3001 gggaacaaag atcagcgcag caagagaagg agtggtaaca agagtaaaag aagatggtac
     3061 aaaaggtgga ctgaaaaaga aatacaggcc ggatggtaat aatatcgtga tacagcaccc
     3121 ggatggttca agagcgggtt actggcacct gcaacataat ggagccctgg taaatgtggg
     3181 agacactgta aagaaaggac aagtaattgg cctcagtggc aaaaccgggt atgcaatgac
     3241 accacatctt cattttttgg tatggcgttc cggtggcggg caatggcaac aggtgcctac
     3301 acgatttcaa acatcgaaag gaataaaata tttaaagggt tggaggaaat atcataatag
     3361 caaaccagag tagctatttt tgataaaaac cattttgtca ttacaggaaa tatatcaaca
     3421 gttcatcgaa gggatgaaaa atacaggttg gctggaatat atcgctgtat ttgccggtat
     3481 cgccagtgta tggttcagcc gtaaggaaaa tatattggta taccctgtcg ggctgatcaa
     3541 taccatcatc tatatttatt taagcttccg atttcattta ccgggtgagg ccagtgtaaa
     3601 cttttattat acagtgatga gtttctatgg ctggtattgc tggctgcaga aagatacccg
     3661 tcaggaaaac atcttacaca tcagttattc caacaagcaa atgtggctga accaggtctt
     3721 gttcttttta ttcttttatt tcactatata tttttcattg gtttacctga aagacaaatt
     3781 ttttgaaggt gccatcccct gggctgatgc atttgcctcc gctactgctt tcaccggtat
     3841 gtggctgatg acaagaaaga aagtagaaag ctggtactgg tggatcgcca cgaacattgc
     3901 ctccattcct ttgtattttg taaagggact ggtgtttacg agtgtatatt atttagtatt
     3961 actggtaatg tctgtttttg gattaacgga atggatcaaa agagcaaaac aacagcatgc
     4021 ttaaaaaaat agtcatcctg gggcctgaaa gcaccggtaa gagtacattg tgcgagcaac
     4081 tggcgcagca ttacgaaacc atgtggtgcc cggagtttgc ccgtgaatat ttattgacca
     4141 atggcaccaa ttacgaatat gaagatctgc tcaccattgc cagaggccag ttggcgctgg
     4201 aagatgagta cagtagtacg ctggaaagaa attcattacc gatgatggaa gaaggcgggc
     4261 accttcccct gttcatcgat acggatatgt atgtgatgaa agtttggagt gagtttgtat
     4321 ttggcaagtg ccatcgtttt atccttgacc agatcgggga aagaaagtat gatctctact
     4381 tgctttgtaa tacagacctg ccatgggtgg aggacgaatt gcgggaatat cccgaattgc
     4441 acacccggaa aaaactgtac tccatttata aagacattat ggttaatcaa cctgtgccct
     4501 gggtcgatat cagcggtgat gatgatgccc gcttgaggaa gggtattgcc gctatagaca
     4561 ccctgctcgg ataaatgtct ttattttcgc actgcatgca aaacattttt caaaagcatc
     4621 accggctgtt attttatggc acctggctgt tactcggcct gattcaatcc ggccttaccg
     4681 aactgcagga tgatgaagct tattactggg tgtatggcca ataccttgac tggggttatt
     4741 ttgatcatcc accgatgacg ggtctgctga taaaaatggg ttatgccatt tttccaaatg
     4801 aactgggggt tcggttgttt cctctgttat tgaatatttt ttctcttttg atcattgaaa
     4861 aattaattga caaaaaaaat cccctgctgt tttatggtat ttcgctgtcg attgcagtaa
     4921 tacaaattac tggtttcgta gccgtgcccg atatcccgtt gatattcttt accgctttat
     4981 tctttttgtt ctacaaaaga tttaccggca attattcact gcttaatact tttttattag
     5041 gtctttctgt tgcttttttg ttttacagta agtaccatgc cgtcctgatc gtcttcttta
     5101 cactgttttc caacatcaaa ttgtttacaa aataccaaac gtatttagcg ggcatcattg
     5161 ctttgttact gtttgccccg cacctgtggt ggcaatacca gcatgattgg gtaagctttc
     5221 gctatcattt atttgaaagc aatgtaaatg cctacaaacc atcttttacg ctggattata
     5281 tcattggcca gcttttactg ccgggacctg ttgccggatt tatcttatta ccggctgcca
     5341 ttttatacaa aacaaaaaat gatacggaaa aagcattacg ctttacgatg atcggtatct
     5401 atgtattttt tctcgccagt tcctttcgcg gaaaagtaga aggtaactgg acctcaccag
     5461 ccctggtatc attaatggtg ctggcccacc agttcctgta tgaaaaactg caatggcaaa
     5521 aaatacttta taaactgctg ccggtaacac ttatactggt agtgtttgca agaatagtaa
     5581 tgatagcaga cctgttacct gttaaagcta taaaagaccg ctatcatgcc tggaaagtat
     5641 ggccgcagga gatgaaagag aagacaaagg gactgcccat tgtattcagt aactcatatc
     5701 aacgggcttc caagtattgg ttttattcag gacagcccac gtattcgctg aacttatata
     5761 aagaaagaaa gaacaattac aatttctggc cgctggaaga ttcgttattg ggaaagccgg
     5821 tttattattt agacaaatat gatctctggc gatttaagga ttcattgcaa acaccaattg
     5881 gctgggttgg ttataaatac gattcttctt ttgcttcttt tgctaaaata agaattgacg
     5941 tttctcccca aaagatccgg gtaaaagaaa aagaagagag ccgtttgatt tgttcgttcc
     6001 gtataccaca gcaatatgag gaatttatac agcaacataa accacaaaat gacacagtaa
     6061 ggattggtat ctttagtacc aaagggtgga tcagggatat ttataccact attacgctac
     6121 aacaacttgt ccgtgacaag actcttccgt taacaatcaa cccgggcctt cggtcgggta
     6181 agtattacct gcggtttgcc attaacagcg gttacagaaa tcctacccat aatagtgata
     6241 agatagagct ggtcattgat taatttttct ttttatccgg tgctggcagc gtaccgatca
     6301 cccgttgtat atcgggatcc gtttgaaggt tattcatttg ttgcacaata actcttgacc
     6361 ggtaaccaat ataggaagag gggggtttca ctttgtatcg cttgggatta ggcagacagg
     6421 ccgctatcat agccgcttcc tgtttagtta aattcttcgc cggtttatta aagtattcct
     6481 gtgcagctgc ttcaatgcca aacaaaccat cacctgtttc aataacgttg agatatactt
     6541 ccaggattct tttctttccc caaacctgtt cgatcataaa ggtaaaatag gtttccagcc
     6601 cttttcgtat ccagcttctg ccctgccaga gaaaaacatt tttggccacc tgctggctga
     6661 tagtgctcgc cccatggatg cggccgggct ttcgctcatt atacttcatc gcttttttga
     6721 tactcttcca gtcaaacccg ctatggtcgg gaaataattg atcttcggat gcgatcactg
     6781 ccagtttagc gtggggtgaa atattaccgg tattgatata atcccgtttt aaaccgtgcc
     6841 cgctaaccca gcttaccaac tgcgtaatcg ttatgggagg attcacccat ttaaataaaa
     6901 taattaagaa aagctggata atgaagagcc atataaaaat ccgtttgacc catttccagc
     6961 atttgcttaa aaaacttaca ctccctcctg gtttattttc tttactggtt gccataaaag
     7021 ataagcccgc caattaaaac gaggccaaaa ataataagta taatcccgct gatacgattc
     7081 agaatatgaa tattatgggg cgtaagccgg tttcgtattt taccggctaa aaaaactttc
     7141 accaggtctg ccagtaatac aatcgccagg gccgctgtgt agataacaat tttatgcttg
     7201 ttggagaaat ttatcaggga ggtggatatc gttatccaga agataatgac tcccgggttc
     7261 atcaggttca taaaataccc ggataaagaa attttcagca ggtctctttt gcgttgtttg
     7321 aataccagtt ttccatcttc atttacttta accttcttga aataaaggaa aaaaattccc
     7381 atcaatatga gcaggacact tccggctata ccgatctgcg tttcatacaa ccgtaaactg
     7441 cgaaagagtt ccgtaaacac attggcaaca agtaccagtg taatatcact ggcagaaacc
     7501 ccaaaagcaa aagccatacc tccctggtgt ccgttattca ggctctgttt aataacagaa
     7561 aataatatcg gacctactgc aatactcagt aataaaccga ttgttattcc ttttaacagg
     7621 gcttctacca tagaatttga agtacagggt tagggataca aagtacgtaa tgtcctgttt
     7681 attgttaagt cctgcatcaa ttgcttttcc gatactaaaa aaatatttgc cggcaaagta
     7741 tgctgcagtg tgtattttat acttcttaca ggcttcctgt agcaagaaac cgttcccatt
     7801 cttccagcat ctttcctttc atcacccgtg ggaatttatg ctggctgccg agtttacctt
     7861 tcagctccaa gaatttcata aatttttcct cgggcaatac ctccaaaaaa acttctttga
     7921 gggcactgtc tctttctgtc gcatagtcat cattcagtac ccgcagctgt tcatctattc
     7981 ttttgcgcag cttgtccgta tccaccttat catcggtggc cacataccag cgatgggcaa
     8041 aaaaattctc gtagggaaaa cctacaaccg tgtattccgg aatgctgaca ttaagttctt
     8101 cacttaccag ctcaatggcc ttattcatat tatcaacact caggtgttcc ccggtgagac
     8161 ttaaaaaatg tttggttctt ccggtaatac acacttcact tttttccaga tcaacaaact
     8221 taatggtatc acctatcagg taacgccatg taccggcatt cgtactcatc agtaaagcat
     8281 actctttccc ttcttccact tcatgtatca tctttgcctc agggttgtca actatcttcc
     8341 catcatggtc aaaattctta tcatcaaaag gcacaaactc aaaaaagata ttattattaa
     8401 gaataagctg catacccctt gcatgctctc ttgtcttaaa accaataaag ccttcgctgg
     8461 acaaataatt ttcgatgtat acaatcggtt tgcccaataa tttttcaaat ccctttttat
     8521 acggctcaaa tgctacccct ccgtgaacaa agaagctgaa gttgggccat atttcgtgga
     8581 tattatttaa cttataatgt tctatcacca tttccataca catctggcac caggccggca
     8641 caccaacgat ataaccgata tcccattctt tggcatgttc tacaatttcg tttagtttct
     8701 gattccagtc gctcatggca gcaatgcgac cgccgggttt ataaaaattc tgaaaccaga
     8761 aaggtctttt tttagccaga atgccactta catctccggc ataccaaccc gctttccctt
     8821 ttttcagatc agtagcccct cccaacatca aaaagccttt tgtcatggct cttttattgg
     8881 catcttcata acggatcagg ctaattaact ggcgcaggta attaatagta ttactgcgta
     8941 acaggtcttt ggtaacagga atatatttac tggctgcttc ggaagtgccg gaactcaatg
     9001 cataatactt gatctttccc ggccaggtta catctggtac tccctcgagt gttttcttcc
     9061 accactcttc ataaatttta ttataatcat gaaccggcac cagttcctgg aatttcttgc
     9121 cgggatgttt actcaataat gcttcatcaa aatgatatcg ttggccgaat tctgtaaacc
     9181 gggccttttt caataacttt tttaataccc ttaactgctg ccgcctgggg tcattgggag
     9241 gaatgcgtaa cgcagcagca atagctttcg gtaatttaat atctaaaata gtggccattc
     9301 gttacttaaa gattatatct aaagttatgc attgttattg ttgaaatagc ggcgaaaata
     9361 agctgcagca aagttctgta atttcatcca ccaagcctcc ggtatgttcg ccatattagt
     9421 tttaaatcag tggaaggttc gtagtcccgt gcataccaat agtccatcat ttgcaggctt
     9481 tccggtgaaa gttgttgtaa ggatgtacct ggtacaccgt tacaggcaat aaccggtgtg
     9541 tgcagtgacg gaagttgtgt ttctggtact gcatatccca cccaggtttt ttttgccagc
     9601 aataccataa aacaattcat aaaaaagttg actggttttt taacaatgaa aagttgaacc
     9661 gggaaagtaa taataccaaa acccgcaagg cttacatcaa ttaaccgttt taacctcctg
     9721 ttgtaaggat cagctaattt atatccattt tctgttgaca ctgcctctcc tgaactgtct
     9781 tttgaatcgc tgccgacaat actgccgcta ttgacagcat gaatcttggc aatgatgccg
     9841 ggtggtaatt gttgtatagt ttggattata ccggcaaagg acaggttgcc ctggcaaaaa
     9901 ataatttccc ggaaaggcag catttgcgaa agttgattta ttttttccag gctaccaatt
     9961 gcagttgtat cgtttgttgt tacagcaagc cttcccagta ccctttggtg taaacctgct
    10021 tcttttaata aacgtattgt attttcatat tcatcaacag accctgcaat taccgtagct
    10081 gctttgtctt ctttttcctt ctcgctgttg actacattgg tttgtaccaa tatccaccgt
    10141 aaaaagctga tcaatgcaaa tgcaagcagg gcaccaaaca acacaatggc tcttgaaaac
    10201 cggtattgct caggtaataa tgaataggcg gccaataata cgatggtggc aattgccgat
    10261 gaacgtatca actctcccca tttataccat ttgtcataca agcccgcata ataagcagtc
    10321 accaggtaaa aaacagtata ggccggaata gcaatccaca atagtttatt ctcatattct
    10381 atatcagttc ggacatactg attccagata tttttgatca gccagaaaga gaataatatc
    10441 agtaatgcgt ctatcaatgg caagccaatg cggcggatga aacttacaac agctgtgagt
    10501 gcagccctga accagattgc actatgaatt aataaattaa acagtccggc cctgcttccc
    10561 caatagtgct tgcggacaaa aatgctcatg gcgttataaa acatccgcac ataattcagg
    10621 cttccctttt tagtgctctc tcccttaaag tgtataatgc tgctttctgc aaaatacaag
    10681 ttcttatacc cggccttttg aatccggtaa ctcaggtcaa catcttcccc atacataaaa
    10741 aaggtttcgt caaaacttcc tactttttca atcacttcct tttttatcat cataaacgcc
    10801 ccagccaaaa catccacctc ctggttatta ttttcatcca ggtgaccaag atgatatatc
    10861 gaaaacgttt ttgagcgggg aaacaatttc gccaggccaa acaatttata taaagaagta
    10921 agcggggacg ggaatgctct ctttgattct ttcaaaaaac gaccgcttcc gtccagcatt
    10981 tttataccca atgctcctgc atccgcctgc tggtctaaaa aagaaataca tttttgaaaa
    11041 cagtcttccg gaacaatcgt atccgggttg agaaacaaaa tgtatttgcc cgagctttgt
    11101 ttcaatccct ggttgcaggc ttttgcaaat ccgatatttt cggtattagc aataaatctt
    11161 acaaccggaa attttggcgt caggtagttt atgctattat cagcagagca attgtctatt
    11221 acaattactt ctgcctgcat gccctcaatc gccttcagta cggaacaaag gcactgttcc
    11281 aggaaatgtt ttacattata attgactatg attacagaaa gctgcaaatt atatgaagta
    11341 taagatacga agtacgaagt ttttatgaac agttcaaaat gaagtgcctg taataaatcc
    11401 gataaaatca ttttaatcaa agtaatctgc agtctatctt tgcccatgct caaaaccact
    11461 ctcatagagg ctgccaaagc aggtgccgca gaaatagtcc gttttttcaa cagtgatttt
    11521 aagatcagta acaaagaagg agtgaacaac ctggtaacag aagcggatca tgctgctgaa
    11581 aaagcgatat tatccgttat caaatcacat ttcccggaac accagatcct ggcagaagaa
    11641 accggtgaga tcatacagga ttctggttat aaatggatca ttgacccgat agacggtact
    11701 gtaaattttg cccacggtat tccgttaaat tgtgtttcaa tcggaattga gcatgcagga
    11761 gaaattgtaa tgggagctgt gtataatccg catctgaatg aattttattt tgctgaaaag
    11821 ggtaaaggcg ctacgcttaa cgataaaccc attcatgtca gtagagaaac ccaaacgatc
    11881 aaagcctgtt tggttaccgg ttttccttat acctatatta atatgccaaa cggcccgctg
    11941 gaaatttttg aacgatttat cagaaaaggt gttcctgtaa gaagattggg ttccgccgct
    12001 attgatcttt gctgggtggc tgccggaagg tttgatggat tttatgaaca taaactggaa
    12061 gcatgggaca gtgcagcggg atatttaatg gtagaagagg ccggtgggaa agtaaccgac
    12121 ttcactggtg gtaagttctc cgtataccag caccgggtcc ttgctaccaa tggaaaaatt
    12181 catgaagaaa tgctggacat cattaataac aagaaagaat tatgagtacg gaaagaacag
    12241 aaataagcag cctcggtgaa tttggactta ttgaacattt gaccaaaaat attgaattgc
    12301 aaaacgcctc ctccatttta ggggtgggtg atgatgcagc cgtgatcgat cattttggaa
    12361 aacaaacggt gatcaccact gacctgctgg tagaaggagt tcattttgac ctgatgtata
    12421 cacctttgaa acacctgggt tataaaagtg tgatcgttaa cctgagcgat atatatgcca
    12481 tgaatgccat tcctacccag atcattctga gtattggctt cagtaatcgt tttagtcttg
    12541 aagcgctgac cgaattttat gatggtgtat atgccgcttg tgaaaaatat ggtgtggacc
    12601 tggtgggtgg cgataccacc tcatcgcaaa aaggtttcat tatttcggtc acggcgattg
    12661 gtgaagtggc tcctgataaa tatgtgaaaa gaagcaccgc tcaaaaaggc gacctgctat
    12721 gtgtaagtgg cgaccttgga gcggcttatg ttggtctgtt attcctggaa agagagaaga
    12781 agatctttat ggaaagtccc ggagtgcaac ccgacctgga aggagaaacg tatgtgatag
    12841 ggaaattgct gaaaccagag gccaggaagg atattgtgga attctttgct gccaaagaaa
    12901 ttactcccac atctatgatg gatatcagcg acggactcag ttcagagata ttacatattt
    12961 gtaaagacag tactcttggc tgtgtattgt atgaagaaaa gattcctgta tcggaagaaa
    13021 tgaaaaaagc ggcttttaaa tttgaaatag acccgacggc ctgtgcgtta agcggcgggg
    13081 aagattatga attattattc accgtttctc aatcagatta tgataaatta gtactgaatg
    13141 agcaaattag tgtggttggc tatatgacgg agccggaatt gggggcacat atcatcacca
    13201 agggtggtgg caaacatgcc atcacagcgc agggctggaa tcatatgaag cattaagaat
    13261 aaaggattaa aaaagaatat ggggagatta cagattttac tggctttgtt aattgctttg
    13321 acgggttgtg gtgtgagcaa atccgcattt tctcctgtaa aaaaatattc gcctcagcag
    13381 ttagagaggg attattcgct ttaccagcat atactggaac agcatcatcc aagcctttac
    13441 tggtacacca gcaaagacag tatggatctt tattttatgt acggcagaca gcagttaaaa
    13501 gattcgatga cggagcctga gtttagaaaa ctacttagtt atgtcactgc taaaattaat
    13561 tgtggccaca cttccatccg caattccaaa gcctggtcca aatacagtga tacggtccgg
    13621 ttgggaaaaa tgttccccct gagtatgaaa gtttggaatg ataccatgct ggtaacggct
    13681 aacctgaacc gcaaagacag catactgaaa aggggaacaa tgatcaccag aataaatggc
    13741 aaggacatac caacccttac agatacactc ttcgaatata tttccacgga tggttacaac
    13801 cggacccata aatatcaatc actcagcaac cgtggttatt ttggctcctt gtatacatta
    13861 ttatatggtc cttcggaaaa atatacgctt gattatattg acagcaccgg gcagcaaaaa
    13921 tcaataacca tccctattta caacccggct gcagacacag caggcaggat gatggttcgt
    13981 ccgccggcaa gaataccgcg gccttccaaa cgggaaagaa gacgccaaca gataagcacc
    14041 atccgtttat taagaattga tacggttaat cactctgcga tgatgaccct ctctactttt
    14101 ggcaggggat atggattgaa aaaattcttc cggcagtcat tccgtgcatt acaacaaaaa
    14161 aatattacac atcttattgt ggatgttcgt ggcaacggag gtggcagtgt aaacaactct
    14221 acccagctta caagatatat tgccaggcaa ccttttaaaa taagtgattc cctgtttgcc
    14281 atcagaaaga gaagtaacta tgaacagtac atccagcatc atttctggaa taagctgttt
    14341 atcactttgt tcacccgcag gcgaaatgac ggtaattatc atttcgggta tttcgaacgg
    14401 cattatttca ggcccaaaaa gaataatcat tataacggta aggtatacat cgttaccggg
    14461 ggaaattcat tttctgccac cactttattt accggtgcat tggttaagca gaataatgtt
    14521 attgtagtcg gcgaagaaac cggtggcggc gcttatggta attctgcctg gcttattcct
    14581 tatgcaacac tgccggagac tggtgtacgg ttcaggctgc ccttattcag actggtaatt
    14641 gataaaacag tacctaaaaa cggcagaggt gttcagccgg aaataacatc attccccagt
    14701 atcgaagcca ttcgtcgcaa tgctgatttt aaaatagaga ccgtgatgga gctgatacaa
    14761 aaagataaag aggagaaaaa gaactgatct tcaggatagt aatcccgacg gattgacctt
    14821 ttgcctactc ctcctcatcc ttcataatca taaatccatc agcatccttc agaaaaggaa
    14881 gtttttccct tatattatcc agctgttttt tctccagttg aatagtaaac acatcctctt
    14941 cgtctttctt tgtatagagc acttctccca tcggatcaat gatcatgctg tcgccactat
    15001 gataaatatt gttcccgtca ttacccaccc gattaacacc gatcacataa cactggttct
    15061 cgatagccct tgcctgcaaa agtgttttcc aggcaagact tcttctctcc ggccagttgg
    15121 caacatagat caatacatca tactcaggtc cttcgggttg tgactgttgt cttgcccaaa
    15181 ccggaaaccg caggtcataa cagacctgca ggttgatctt ccatcctttt actgatgcga
    15241 tcaatctttt ggtgccagcc gtatagtgat tatcttctcc ggcaaatgca aagcgatgac
    15301 gtttatcata tacgccatac tgaccattgg gcagcatcca tattaacctg ttgtaataat
    15361 tatttgcttc ttttatgatg agacttccgg taagaacgat tttttttgct gccgctgttc
    15421 ttttcatcca ttgaaccgtt tctccttcca tagtttcagc aagctcctcc ggcttcatac
    15481 taaaaccggt gctgaacatt tcgggtaata caaccacctc tgtttttatg gagatgctgc
    15541 tgatcttttc ttccagcatt tggaggttgg caatcttatc ttcccagtga agatttgttt
    15601 gaatggttgt tattagtagt gctgacatac ttttaattct tcggggttct tgtaaaagta
    15661 cttgtattca ataaaaaagc agcggcctta gaaaagaccg ctgccaacca ttaaccatct
    15721 gcttttaacc tacttaaaaa tgttatagcg gagcaatacc tcgtgggcat tggatcctgc
    15781 atcgaataca ctgatcggtg ttttatatat atcaaacgag tatcctacag taaacttctt
    15841 attcaggttc acaccggcag agagaataaa gctttgcttg gcccgcagtg ccaatcccca
    15901 ccagaacact tcgctatgtt ctactcttat tccaccctgg aactctaaag gagcattggg
    15961 taaatagata aataaaaagt tgggcgttac agaagtagct tcgtccacat tccatttata
    16021 agaaccatgg aaataatagt gacggtacaa gcgggcctct tctgaaggac ttacagtacc
    16081 tgtatagtaa ccaagctttg actgaaccag ttgagcaacc gatgccccca gttgtaattt
    16141 cttaccggtg taagaaacac caaagcctgc atcaaatttg aaacggttgt cacttgttcc
    16201 caaagcagga tctgaaccca gcgtttcaga aagtttggcc ctgtcaaggg aatactgcag
    16261 acctttagct tcgataccca atgaaaactt accaccgttt gccataggta tatgtttggc
    16321 aaattgcagt gcaatacctg ttcttgaagt tggaccggtt ttatcactaa acaggtaacc
    16381 gcctaaaccg atcttctgct ccggtaattc aaaagaacca aaaacagtag tcgttttggg
    16441 gcttccgctg ataccgctcc attgacttct gtaagtagcg ccaaccattc ctttttggta
    16501 aacccctgct gtagagggat tgtgcaacac tccctgcata tcatacatgg aggaagtctg
    16561 taactgttgt gcattaaccg ttgtaacaac tccggcaaat actgcaaata atatataacg
    16621 tttcatgtta gtgttttaat ttttgtatta tctcaaaatt gttacgtcac ctttcagtgt
    16681 aatggatgcg ccgttgatta ggcggtatgt gatcatataa taataggtac catctgccac
    16741 cggcttgcct ttgtaagtac catcccattt gttctggtag ttatcatctt tgaaaacaat
    16801 attgccataa cgattgaata cggtggcaaa cacctgtctt gtacaggtac cgccattgtt
    16861 ggtaacgatc cacacatcat tttgtccgtc accatttggt gtaaaggcat tcatcacttt
    16921 caggcaatag ggaattacat tcacgaccat gttatcagta gatgtacaat tattgttgtc
    16981 ccttacagtc agggtatagg tagtggtcgt attgggtttc gcaacaggag tataggtgtt
    17041 ggctccggtt aacgtattgt taggagtcca tgcgatagaa gaaggtgttg atgtaccgct
    17101 tccttccagt gttacttcat caccatcaac gattgtttta tccggacctg cactgatgat
    17161 cgggttcggc ttaatattaa cattcaccgt ctttgttctt acacaaccgt tgaggctggc
    17221 agtaaccgta aaggctgcac tgttgtttac ggtagcaacc gggttgctga tatttgttgc
    17281 agatagtcct cctgcaggtg accatgcata agttgttccg gtaccattag tgctggccag
    17341 cattggtaca ggtgcgccgg cacaaaccaa tgtgtcgtta ttcgttgtca gtaccaggtt
    17401 atcattaaat ccaacattga ttgttttagt taacaacccg caataagaat cttttatcgt
    17461 aatagttttg ggtccttgcg ttaagcctgt aaatgtatta gaagactgga aagcccctcc
    17521 attaatacta tacaaataag gagaaattgc acctgttgca ttgaccgtga tacttccgcc
    17581 ggttagattg gtacaggatg cattgacaac attttcagtg gagttcactg cgatatttgc
    17641 cgggttacca aattccatta taggcgcccc acttacgttt actcctgtca caaacaatct
    17701 cctgtttgca aaaggaatag cagccggaac tgtctttggt gcaaaggctg ctgtttcaaa
    17761 aaggcagctg gtaggtggaa cttccaaggt ccaggtagcg ccgccatccg ttgttttaca
    17821 catcaggccc ggtgtcatta cataaccatt gtttatatca tgcattacaa tagcattcag
    17881 attacctaca cccaatgtgc cgatatttcc ggatacatct gtccaggttg ctcctccatc
    17941 cgtcgttctg aaaatcactt ttctgggaaa cccgttgcca ataacaaaaa ctgtatttgc
    18001 atccagggca atcatgtctt tatacgttat ttgcggtgtt acgccaggag taggaagagg
    18061 aagtttgctc caggtaacac cggcatctgt tgttttataa atagtgtccc tgttgccggc
    18121 agcatatccg attaaccggg ttgggaattc aatttccgta aggttttgtg ccaaagaacc
    18181 aactgagaat ccactgtaaa cactccaggt agctcctcca ttgaatgatc tgtaaactct
    18241 tgaggttctg gaggcagaag gcactgagaa actggtataa ccaattgccc acacactgtc
    18301 attacctacc gttgcaagat cattcatttg catcagccca tctgcataca cagggtctaa
    18361 tgttggtaca gcgggtggcg ccacgtttaa atcagtagta taataaatgg acccgttgct
    18421 aacggcaaag tatgcctggg caggatttga atttctataa gcaaatccat tgatgctata
    18481 gaaagaagac gcaaaatcag gccttgttct atcccaccag gttttgcctc catctgctgt
    18541 tacattaatt gttgagctcc ctgcagctac accattatta cagtcagcaa attccatttt
    18601 agtaaatccg gcactgagcg gaacggtaac ataattacta ctccatcccg gtccacccgg
    18661 tattgaatct gccacaacac cgagagaacc catagataag aatttaccat ttggagcaat
    18721 atcaagtgcc cacacaccat tgttactata gttttgtccc ggaggtaaac ttcttgattc
    18781 cacccagttt cttcctgtat ccacagaagt ccacatacgg gcaaaatttc cgtttgctat
    18841 cagtgtgcca ttactagtcc ttctcatttg gtaaggatta gacgctaaca gaacctgcac
    18901 agcaggtaca ggcgggtagc ccggaggggg acctgcaggg ccagtattca atatctcaaa
    18961 ttttcccttt tcgtatacac cggggcggtt aacgtttagt gtgctgtcat tcttcccggt
    19021 acgtactctc accaccacgt tattattaaa cgacatcatc acaatcaaag tgtcattgat
    19081 aatattcagg gctctgaact gctggttaac ttgtgataca gttccaaatt ttgatgtaca
    19141 gtttattgtt gccgggaaac cggtataacc cagcctttct ttgctgatag agaagtcggt
    19201 tagtgtacca ttatcaaact tccacaataa gctggcatta tgtgatcctg ttgtttgttg
    19261 cccggttaaa gcacaggtgg tgctattggt tatattgggt atacccaaac ctggaaaatt
    19321 tgaagcgccg cctccactta tatagccaat attattattg atgaactcta ttctttgtat
    19381 ttcttttgct ttagctgtga tgggaaagtc aattgaaggg tatgtagcat tgttgatgaa
    19441 gcctactttg gttgtaccat tggatacagg tgcagccata gaatcccagg tagccccgcc
    19501 attattggta acatacagtt tagggataga atcaggtgta ttccattgtc cgcctatata
    19561 gccagttgtg ggacttgtaa accaaacagc atttatgttc ctcgcattac tggacaacgg
    19621 agtgtttatc aaagtccatg taagaccgcc atctgttgtt ttagccatac aaccaagatt
    19681 gcctaccgca taagcaatag tagagctaac aaaatgtaca tcgctaaaac ttggtttggt
    19741 taaaaacccg ttagccggat ttacaaaagt aaatggccca taagtccagt tggagccgcc
    19801 atctcttgaa cgggcaatgc cgccatctga gccaacagcg atcacattat tgttatcaaa
    19861 aaaatcaaca tcctgtaaag taaacccaaa ctgtttgggg ttactaaact tccatttagt
    19921 ggtatcataa ccctggccta tgacgagcat agaagaaaag gaaaataaaa ttatcagcag
    19981 ataataatgc aatttcccta agcagttctt attcattttt aataactttt tatgttttgg
    20041 aagaattggg agagcaatgt tatagtaaca tttacacccg gtttgtagaa ttaattctac
    20101 ttagtaatca ggataaaaaa ttgatttttg aacttgcaaa attttatgcg gcaaaagctg
    20161 tctattatat tagttatcag tctttttttt ggttccctgt caaatgcaca ggataaatcg
    20221 aaccggggca aagaattttg gctgggctat ggttttcact ggttgttttt aaacccggat
    20281 ataactcccg tgaatagcca ggaattggcc ctttatataa gttcggaaca ggccgctaca
    20341 gttactgtct cagtaaacgg aaccgcctgg acacaaacag tgaatattcc ggcaaatact
    20401 gttgatgcaa ctatcttgat tcccaaaaca ggacctaatg atgcccgttt acttacagac
    20461 ggattctcta ataaaggaat ccatatagtg agtgatgttc ctgttgcagt atatgctcat
    20521 gtgtatgtaa accagggatc gggtgctacc atgctgatgc cggtggaaac ctatggctac
    20581 tcctattcat cagttaatta tcgccagaac acttcaggct cttctttacc taatatcagt
    20641 cctaccacac aaaatggccc tgattggtat tcctggtttt atgttgtagc atcagaagat
    20701 aatacaaggc tggaaataac ccccagcgat accacaaaaa atgggtggtt gccgggccaa
    20761 acctatacgg tcaatctcaa caaaggtgaa atatatagtg tatatggtaa gatggtaccc
    20821 ggcaatagcc aggcatgggc tgccagtaag gatatgactg ggagtaaagt gattgccgtt
    20881 cccggttctg atggtaactg tcaccctttt gctctttttt ccggttccag cggtatccgc
    20941 atttgccggg gtgatggagg tgaatacatg caacagcaaa tgtttccttc acaggcttgg
    21001 ggtacccgtt atctaaccta tcatactatt aataatacga atacagacat acttgaaaca
    21061 aacaggaatt attacagggt ttgtgtttca gatccaacag ccgttgtaag aagaaacggt
    21121 atacctctta caggattggt taacaacttt tattatgaac tcatggattc aaccggtgga
    21181 gattatattg aatctgataa accggtttta gtttctcaat acaccgtaaa cgacaaccag
    21241 tgctggaact ttccaactac aacaccttct cctccttcaa acggagatcc tgaaatgttt
    21301 tacctgagcc cgatagaaca aggtcaaaaa tcagtacgtt tttttgccag cagacaatct
    21361 ccggctattt cttatgtcta taccaatatt catgtgccca ctattgctgt tggctcatta
    21421 cgagtagatg gaaatgttgt tccggcttct cagattgtag tacatcccaa taacccggct
    21481 tactccgttg cattggccag gtttacaggt cccgctgccc aacatactat cacctgtgat
    21541 tcagctttta ctgcgaccgt atatggcctg ggcaattatg aaagctacgg ctataatgtt
    21601 ggtacactga tcaataacct gaatcattac agcgagatta ggaatacttt taacacaaca
    21661 ggcgcagttg atacctttac ctgtcccaaa tccccggtaa gactttttgt aaagctgggc
    21721 tatcctgcca attctattca ttggaagttg agccaggtaa caggtatgtc gcccaacgct
    21781 gattcaatta tcagcaaccc ggtgcctgtt cgtacagaaa ttattaatgg gcgaacctat
    21841 tacgtataca ccctccaaca ggattttatt tttgctcatc cgggcactta ttatattcct
    21901 gtttctttta ccgcaacggt tatagaaaat tgcagccaga ctgaaaatgc aactgtgaaa
    21961 gtagtggtga agccagggcc tgtagcagat tttacttttc cttcacccgg ttgtttaaca
    22021 gatagtatcc gttttaccgg cgcttctgtt ccgggaacat tcaacctggt caactataac
    22081 tggttgtttg atgataacag tactgcaaat accgttaatg ccatgaagct tttcaccatg
    22141 gcaggtaatc aaaatgtacg ttacacgatc tttgcagata atggttgtat aggtgacacg
    22201 atcaaaacag ttgcgataca ggaaagcccc gttgctgcat tcagtattag tggaaatagc
    22261 tgtcagcggg attctgtaag gatcactgat gcttctgcaa tacctgccgg tactattacc
    22321 agctggcaat ggaattttgg tgatggcaac acggcaacac tcactaacaa caatccgttc
    22381 tatcatcaat acactactcc cggcaattat actatttcat taatcactgt tgccgccaat
    22441 ggctgtaaga gtgataccgc ttttgtgccg gtcacaatat taccaaagcc ggttgctgct
    22501 tttactaccg ggggaaatat ctgtcttgga gattctgtga ggtttattga tgcttcaacc
    22561 gcacctgttg gtaccatcac cagctggcaa tggaattttg gtgatggcaa taatgccacg
    22621 tacaccaaca ataatccgtt ttatcatccc tataccaccg caggaaatta tcctgtttcg
    22681 ctgattgtaa ccggtagcaa cggttgtatc agtgatacgt tcaggcttac agttaatgta
    22741 agcagtaagc caaccgcctc ttttactttc attggaaaac catgtataga tagtgtacaa
    22801 acttttactt cttcagtacc cacaggtggg tctaatccac ccacctggca ttggaacttt
    22861 ggggatggac aaacttttaa ttccactact gtaaacacgg taacgcattc ttatactgcg
    22921 acattatcca atattgttgt aaagcattgg gttagttaca gcgccggttg cagcagcgat
    22981 acttcttttt ccactatccc gcttattaat cctaacccca ttgcttcttt tgctgttact
    23041 ggcgataccc tttgcgaaag aaaaccaatt acactgacat catctgtaac gggagtaagc
    23101 agctggaact gggatatggg taatggcacc agcaatgctg tgcctccatt caatcattct
    23161 tatactgctg caaacactta taccatcagc ctcttcgtaa gaacagcagc cggttgtggc
    23221 tctctgcccg ctacacaatc agtaacaatt aacccgacac ctgctgttaa tgctggtccg
    23281 gataaattta tcaggatagg aacatccact acattagacg ccactattaa caatgcggca
    23341 aattataatt acctgtggac cccttcacta tacctgaacg ctacgaacat actgaacccg
    23401 gtatctacac cggataacct ggttacgtac accattcagg ctattgataa actgaataat
    23461 tgtatcggta ccgatgatgt tgttgtaacg cctgtttcag atatatttat tccttcaggc
    23521 tttacgccca ataatgatgg gaaaaatgat aaatgggtta ttcccggcct ggcattatac
    23581 ccggaagcaa gagtcactgt attcaacagg gccggacaaa taatatttga tggcaaaggn
    23641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc acaatgccct gtttgcaaat
    23761 gcatcaccgc caaacagtca aaatcatact ttatacctca gtgccgaaca ggccgcaaca
    23821 gtaaccgttt ctgttaacgg aacaagctgg agccagacgg taaacattcc tgctaatact
    23881 gttgactttt ccattatcat ccctaaaagc ggtgccgatg attcaagaat tttgagcgag
    23941 ggtttgttta ataaaggcat ccacattgtc agcgatgttc cgattgttgt atactctcat
    24001 caatacaacc ttttttcttc tgctgctact atgttgatgc cggtggaaac gtatggttac
    24061 acctattatt cattgaacta ctcacaggtt tctaattatc cggacagtta ttcctggttt
    24121 tatgtgattg cgccggaaaa taatacaagg gtacttatta ccccttccga ttctacacaa
    24181 ggtggttggt tacccggcca aacctatacg gtgaatttga ataagggtca gctgtacaat
    24241 gtatttggaa aaagagccga cacttataca ggaaaagata tgagcggaag caaaattgtt
    24301 tctgtagcag gagctgatgg taattgtcac ccggtagctg ttttctctgg cagcagccgt
    24361 gttgttattt gcaatggtaa cggaggagaa attttgcaac aacaaatatt tcctgccaat
    24421 gcctggggca cccggtatat aacttatcat acggtgcaga gtgcaatggg agacattaca
    24481 tcacctctcc ttaatattta ccgggtggcc gtaagaaacc cggcaaacat tgtaaaaaga
    24541 aacggggttc cgctaacagg gctgatcaat aatttttact atgaattttc cagcacttca
    24601 ggagattata ttgaggcgga ccaacctata cttgtatcgc aatacactgt ttctgctaat
    24661 gaatgtaccg gttcatccac cggaccgtat ggtgaccctg aaatgtttta tttaagccca
    24721 atagaacaag gcgtaaagaa cagtgttttt tacagcaccc gcagggaggc tattgatttg
    24781 aattttatta atgtcattat accacagggc ggtttatctt ctttacggat agacggtacg
    24841 gccgttctcc ctgctgaata tatcacacac ccggttaaca ataattacgc tgttgttgta
    24901 aaaagacttt ggggacctgc agcacaacac agcgtcaata gtgattcaac ttttatcgcc
    24961 actgtatatg gcgttggtac attggaaagc tatggctata atatgggaac ctatgtaaat
    25021 aatctgaatg cttatagcag tattaaaaac tcattgaacc aaaccaacca ggcagatact
    25081 ttcacttgcc tgaaaactcc gttccggata actattaagc tggcatacaa agcatcatcc
    25141 atccactgga agctcagcca ggtgcccggt ttaaatccaa atacagattc aattatagta
    25201 aaccccatac caattgattc ctcttttatc aacggaagaa aatattatac ctacaccctg
    25261 caacaggatt tcacttttgc tacagctggt acatattata taccggttac ctatatggct
    25321 cctgatattg atgcctgcaa ccaaactgaa tcaacaacag taaaggtgct ggtaaaaccg
    25381 gggccaagag cagattttac ttttaacgct cctgcgtgcc tggctgatag tattcgtttt
    25441 accggaacag ccgttcccgg ttcgtttaat cttatcaatt ataactggct gtttgatgat
    25501 aacagcaccg ctaatactat caatgctgta aagcttttta caaataccgg ttctcaaaat
    25561 atccggtaca ggatttatgc tgataatgga tgtcttggtg ataccactaa aacaataact
    25621 atatcggaaa gcccaaccgc ttcttttagt attagcggag cccctttctg tgaaggcaag
    25681 cccgttacca ttacttcatc tgcatcaggt atcagttcat ggaactggga gcttggcaac
    25741 actaccagta cagctgttcc gccttttacc cattctttta gtaccggcac ttattctgta
    25801 aagttggttg cgaaaacaac ttccggttgt ctttctgata cggctgaaca aaacataaca
    25861 atttatccga cgccggttgt taatgcagga gctgataaaa ccatcagggc cggtagttca
    25921 gtaacactgg atgccgccgt tactcccgcc ggtgatcata attttagctg gtcgccttct
    25981 ttatacctgg atgctcctgc aatactaaat ccggtatctg caccccttgt ttccaccagt
    26041 tacacaatca gggtagttga cagacaaagt ggttgctccg gcgaagacga tgtgaccgtt
    26101 actgttattt cagaactatt cattccaaat gcttttacac caaataacga tggccggaat
    26161 gataaatgga tcattcccgg gctggcattg tacccggaag cagtggtttc ggtttttaac
    26221 cggggaggcc ggaaaatatt tgaatcaaaa ggatatacga ataacccctg gaatggaact
    26281 tttaaaggca taagtcaacc caacgatgtg tatgtatacc tgattgagct cagggatgaa
    26341 gccaaaaaaa ttataaaagg aaccgtagcg atcatccgtt aacacgatag aataaattct
    26401 acaaaaagca gcaataaatt atggaaatta gtagattaaa tactagcagc gagaccgaaa
    26461 ttttgaaaaa aatgccgcca aaaattctca tagcagatga ccatagtatg atccggaaag
    26521 gactaaagct gcatatacaa cttaccctcg gctacaacga tattgaagag gtggccacct
    26581 gtaatgacct gatgaaagaa ctcgtcaaga aaaaatacac tcacctggtg ctggatatta
    26641 ttttatctga tggcagcacc cttgaagtga tccctaatat acgcagggta tatcccgatc
    26701 tgcacattct tgttttctcc atgcagcctg ccgaaatata tggtgaggca cttaagcaat
    26761 atggcattaa ttactatctc tccaaatctg ttggcgaaga agaaatgata caggtgctgc
    26821 agaaattcct gcagaacgaa accccgatta gaagaaacga tatccatcac cacgacaacc
    26881 ctttcaaaac actggcccca agagaactgg agatccttca ctatgtatta aaaggaattg
    26941 gcacaaaaga aatcggggaa acactcaatt taaaaatgaa taccgtctct actattaaaa
    27001 accgcatcta cgaaaaaacc aatgcgggaa atatcaaaga actcatggaa ctggccaccc
    27061 tctacaatgt aaattattaa gcgggatgct gtaacttgtt atggcaatca ccttatgcaa
    27121 aggttcctga ttttactata tttcattctt gcgctggcga atcccgccat gtcccaaact
    27181 gccggaaact cacatctgcg tcaatatacg actgacaatg ggttaccatc aaatggcatc
    27241 aaggggctgc agtgggatga aaaaacaggt tttttatgga ttgcaaccga agccggtatc
    27301 gttcgcttta atggagttga tttccgctcc tataccaagg aaaatatgcc ctccattacc
    27361 accgaaagga tgttgtttat ggtccggaac aggaaagagc atatttatat atcagatatg
    27421 gccggtaata ttttttggat caaccaaaac aaaccggtgc tttggaaaaa gggagcggag
    27481 aatgttaatc cgtattatgc gaattattat ttactgtccg tttccgatac tttttttgaa
    27541 agacataccg gcgttgccaa caatacccgt ttttcagcag tcaccgacaa aattgtcggg
    27601 cttagtgata cagcctgcct gcttcttaat aatggcaccc ttttttatca ctcgataaca
    27661 atgccagctc cggtggcatt accttttgag cggggtagtt tcaaatcttt ttttaaaata
    27721 aacgaccagt attttctaat caataataaa aaagaagctc tcctgcttaa taccagcgac
    27781 tttactgttt ctccggttgc agtaaccgat attaatggaa gccgtctgct ttttgaaaca
    27841 ggtaacaacc ctgtattctg ggagcccggt atgaataatc ctgttttggt ggaagaggaa
    27901 aatgcctggc tatttacatt cagggataac aagataacag caacactgct gttcacgggt
    27961 attccttcag acgcactgat ccgaacttta gaatacagcg ataaaaacaa gacccttttt
    28021 atcggtaccg attcaaaagg gctgatcatt attaatcaaa cctctgtaac caccaaaaaa
    28081 agaacagaca gcgtctctaa aaacaggaat tcctattatt cacagattga acttgcagac
    28141 ggtaatgtat taacaaatga aggagatatc attggcgata cccccgctgc tgaaaacaca
    28201 atacctgtaa aaggaaaatt tgcttactat gtttcttcta ccggcgacag tttgttgtgg
    28261 tatagccaat acactccttc cctgggatat tcctgcctgc accggtataa caaagtcact
    28321 ggtgaaataa aagtattcag caagatcaaa cccgaccagg tggtaaccca atcgggtaca
    28381 aactattact tagcgagcaa caccgggata ggtgtgctgg atgcagacag catcaggctg
    28441 ttacacactt atgccggaag aaatgccggc attattgcat ttgctatagc agagatacaa
    28501 ccgggagtaa tagctgtagc cacctgcagc ggcttactcc ggtatacaat cgttaccggg
    28561 cgactggata caattttcag caaagaaaat atttgtgtac ggagcatctg gaagtataaa
    28621 gactatgttt tttttggcac atatggttcc ggctattata tctacaaaaa cggggtgata
    28681 aaacagatgc ccctggataa gaataagtat ttgttatatg cgcactgctt cgttcccgat
    28741 gataatggtt attgctggat cagcaccaac cgtggattat ttaaatccag cctcgaagag
    28801 ctcatcaatg tatttgaaaa taaaaagaca tccgtatact atcattattt cggaaaaaaa
    28861 gatggtatgg aaatgacaga actgaatggt ggttgcactc cctgcgcatt gcgtttaaaa
    28921 aacaatatca tttcatttcc ctcaatggat ggtttgctat gggtaaaccc ggacaaagcg
    28981 attcctgtat tgccggaagg cgatattttt attgatgaaa tacttattga taatgaaata
    29041 aaagacccgg gatatctttc ccaaaaaagc ctgcctgcca aaactggtga aataataatt
    29101 cgcctcgctt tttccgcctg gtgcaataaa gaaaatattt acctcgaata ccagttgaat
    29161 gacaccctta actggaagcc tgttgttttt gaaaatggat cagagatccg gtttaataac
    29221 cttccttccg gggattataa actacggata cgaaaactga atgggtttgg gttcaataat
    29281 tacacctaca aaacagtaga ctttaccata accactccat ggcaccagca atggtggttt
    29341 tttgtactgg cttccctgct ggtgctgggc atgttgtctt tttatttccg tttcagaaca
    29401 aggcaatatg aaataagaca acgaaaactg gaacagcagg tggcagaaaa aacaaaagag
    29461 ctgaaagagc aaaatgaagt cctggaaaaa aacaattcaa taaaaacaag gctaatctcc
    29521 attatcagtc atgatattgt taccccgtta aaatttctga cagtggccgg aaagaacctg
    29581 atagaaaaaa gaaagatgat gccggaagag ctgcaacagg aaacattgca ggagatcacc
    29641 aacacctcac aggaattgca actgctttcc accaatatcc tcaactggat aaaataccag
    29701 aatgaaaacc ggcggctggc aaaagaaacg tttaacctgc aggagatggt aaaccagata
    29761 ttgggtatcc tgcaatcact ggcccggcaa aaaaatatca ctatagataa ccaggtggaa
    29821 aaagaactgg agatctacca gtattatgaa ccattaaaga tcctgatcta taacctgctc
    29881 accaatgcta ttaattttac ggagaaagga acgataacta ttacggctat gcggcagaac
    29941 gatcaggttt ccattgctgt taaagatgag ggcattggta tgagtcccga acaaatccaa
    30001 cgattgatgg ccgaagatgt tgttattact tctgccaatg tggataacag gaagggacat
    30061 ggattaggtt atctgatcat caaagacctg ctgaagacaa tgggagccag gttgcatatt
    30121 gaaagtaaaa aagggcaagg cacaacggtt tcgatttcga tgtttgcttt atagcggttt
    30181 ttcttttcca acgagccctc tcaccaggtc catttcaaac cccttattca ataaataatc
    30241 catcgtcttt ttcttccgta ccaggtattg ttcggctttc agcgatgcgt atttttcatc
    30301 ggccagtttt ttcaacacag ctaagtattc ttcctggctg atttgctgca atgctttttt
    30361 tatacagtat tcgcttacct gtttttgctt tagctcatat tttattttta cccgtcccca
    30421 atgtttcacc cgccattttc caccggcata cgcaatggca aagcgttctt cgttcaggta
    30481 attttcttca atcaatgtgc tgatgatctc atcatgctct ttcttccaca cacccaggtt
    30541 aaacagtttt tcttttacct cgctatggca gcgttcctgg taggcacagt aatgtttcag
    30601 tttctgcagg gcctgttctt tggtgaggtg ttttttatac atgaatt
//
LOCUS       DAORTI010000028        30637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1040_length_30637_cov_6.058825, whole genome shotgun sequence.
ACCESSION   DAORTI010000028 DAORTI010000000
VERSION     DAORTI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 30637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1040_length_30637_cov_6.058825"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1764
                     /gene="miaB"
                     /locus_tag="PLO70_05685"
     CDS             343..1764
                     /gene="miaB"
                     /locus_tag="PLO70_05685"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQZ73984.1"
                     /translation="MLDLMTDKVQDETRQGEAFAPFANDPNQYKKRFYIESYGCQMNF
                     ADSEVVASILNKEGFGATRNVEEADLIFLNTCSIREKAEQTVRKRLTEFRKLKENRKG
                     MLVGVLGCMAERLKSKFLEEEKLVDIVVGPDAYRSLPELVIEASGGQKAVNVLLSRDE
                     TYADISPVRLDSNGVSAFVSIMRGCNNMCSFCVVPFTRGRERSRDFESIIHECKDLFQ
                     AGYKEVTLLGQNVDSYYFVDEAKKETITFARLLEQVALISPLLRVRFSTSHPKDITDE
                     VLHTMAKYENICNYIHLPVQSGSSRILQLMNRTYTREWYMAKVDRIRQLIPDCGISSD
                     FIAGFCTEEEKDHQDTLSIMEYSRYDFSYMFFYSERPGTLAHRRYKDDIPEEVKKRRL
                     QEIVEVQNRLSLESNKKDIGKTFKVLIEGDSKKNEKEWKGRNSQNKVIVFPKGNHDLK
                     KGDYVMVKVNECTQATLLGEIIK"
     gene            1821..3068
                     /locus_tag="PLO70_05690"
     CDS             1821..3068
                     /locus_tag="PLO70_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQZ73985.1"
                     /translation="MEIQIIKNRFGIIGNSPALNYALQVAIQVANTDLTVLINGESGV
                     GKEVFSQIIHSLSARKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGAVDARKGYFET
                     VNGGTIFLDEIGELPLGTQARLLRVLEAGEYIRVGSSKVQKTDVRVIAATNKDLMQLV
                     QSGRFREDLYYRLSTVPIRVPALHDRPEDIHLLFRKFAADFANKYKTSPVQLDDEAKQ
                     LLINYPWPGNVRELKNIAEQISVLSQDKMITAKEFNKFLQPYSNNRLPVLASPGGHTA
                     EFSNEREILYKLFFDMKKDVTELKRMFLDVLQNPEMVAQNQHFINELKELRSQGQEVI
                     QPMPLSISTQPVTQTVQPVMMRNDIHDHVEVEESLNIMDKEKELIIKALKKHKSKRKD
                     AALDLGISERTLYRKLKEYDIEE"
     gene            3068..3592
                     /gene="lptE"
                     /locus_tag="PLO70_05695"
     CDS             3068..3592
                     /gene="lptE"
                     /locus_tag="PLO70_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQZ73986.1"
                     /translation="MNTPKKIFLLAVIGLFSFMALFSNSSCGIYKFNDASVPDSIKTV
                     KVNFIENKATYINPQLSPRLTDKLRQKIVGQTKLTQTNNDNVDWEISGTVTQYSFSTS
                     AITGQQAANNRLTVGIQMILVDHKADKTEKFDVSRSFEFKGNQSFQQAENDLAEEMIR
                     TLTDDIFNKLFSKW"
     gene            3650..4504
                     /locus_tag="PLO70_05700"
     CDS             3650..4504
                     /locus_tag="PLO70_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73987.1"
                     /translation="MNVRLNQLVKSLLQKESLDQCSLPELTAFAEKNPYFGAAQLLLT
                     KKVQTEQPEKYDEQLQKTLLFFHNPLWVEHLLNDTGLAEISKSKKEEETVSQQPEEST
                     AIAPASLQDSIWTDESIPVIVATPQTEATIAEARSSVAEENPAIVPSIQIENAASTEP
                     ALTFEPFFTVDYFASQGVKFKEEEKPVDKFGQQLKSFTDWLKAMKRLPVTEMAKSVET
                     QAEQKVEQLAGQSIADREVVTEAMAEVWEKQGNVAKAIELYSKLSLLEPSKSPYFAAK
                     IEDLKKIN"
     gene            4506..4871
                     /gene="secG"
                     /locus_tag="PLO70_05705"
     CDS             4506..4871
                     /gene="secG"
                     /locus_tag="PLO70_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQZ73988.1"
                     /translation="MTTLFVILIILASVVLGLIVLVQNPKGGGLAGNIAGFSNQFMGV
                     KQTTDVLERGTWIFGGVIAVLCLLSVLFISKSGPSSGPDRTKDATGASVPATQPQPNN
                     QNTLQLNPQTSPVTPDSTK"
     gene            4951..5991
                     /gene="mltG"
                     /locus_tag="PLO70_05710"
     CDS             4951..5991
                     /gene="mltG"
                     /locus_tag="PLO70_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQZ73989.1"
                     /translation="MSKKKIITGVILILLIVAAFIGYKIAGPAVSAKKQTYFYIKTGE
                     SLETVKQNLVSQKIITGKAWFNRVAGWLKYTVVKPGRYEIKDGMSLLTLAKMLRNGKQ
                     APVKMVLVKERTKELFAGKMGKKFDTECDSLQMINFLNNNDSLKRFGIDTSTVMAIVM
                     PYTYEITWNSSPGKIVQQFYTAYKKFWSQERKLKADSLRLTPLQISVLASIVEEETNK
                     KADKYNIASTYLNRLKIGMKLQADPTVKFVTRNFQLGRITGVHLKLESPYNTYLNTGL
                     PPGPICTPSVESIESVLNAPKTDYLFFVASYKFDGSTIFTSNYTDHTKYVKLFHAEQN
                     RRSDSLKKLKVK"
     gene            5994..6962
                     /locus_tag="PLO70_05715"
     CDS             5994..6962
                     /locus_tag="PLO70_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQZ73990.1"
                     /translation="MPLLKKILTSIEASAPVAFIIKKSKSTSLPGFNGIPLFDVINFF
                     IGQVKTIGMTERASAIAFNFVMAIPPAIIFLFTLIPFIPITDSFQQEMYSLIRDVIPG
                     EKDNAVLIKFLEDFINNPRTGLLSFGFLLSMFFSSNAMMGIMRSFDKNYIGFRKRKEF
                     QKRMVAIKITMLLYLFVFASVLLLVARGAVLEWLGLTNKTAIFIINNLRWIVIILLFF
                     ACISFIYRHAPSVHKKWRLINPGSILATFLMLLMTFAISWWVSHFGNYNQIYGSIGTI
                     LIIMALIFINSLVLLIGFELNVSISSLKKIADERKDSMAGGEGISA"
     gene            6997..7605
                     /locus_tag="PLO70_05720"
     CDS             6997..7605
                     /locus_tag="PLO70_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQZ73991.1"
                     /translation="MKKILFSILPVAALGLVTLSSVSDPLPIGAAAPNADLKMKDISG
                     KEVSLNDAKKKNGLLVMFSCNTCPYVIKNQSRTNEIAKYALDKEIGIAILNPNEAYRD
                     DQDSYEAMKQYAVDQGYAWYYLVDKNHTMADAFGANRTPECFLFDKNGKLVYHGAIDD
                     SPSDASAVSRKHLKEAINEITTGKDVSVKQSKSVGCTIKRLK"
     gene            7733..7933
                     /locus_tag="PLO70_05725"
     CDS             7733..7933
                     /locus_tag="PLO70_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ73992.1"
                     /translation="MLSRQTLNRIIIIGFMVLVGFALAKAIYHNSVLGIIMALTSLGA
                     GIYFLYLMAKAKEEMEAGDASR"
     gene            complement(7939..8415)
                     /locus_tag="PLO70_05730"
     CDS             complement(7939..8415)
                     /locus_tag="PLO70_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ73993.1"
                     /translation="MKKLYLLTIFTIVAGMLQAQPVRSIKITELEKIIAESKTPLIVN
                     FWATWCMPCVEEIPYFIEEAKNHEKDSVQLLLVSLDFKELFPKGVESFAAKRKITAPI
                     LWLDETNADYFCPKVDTKWSGAIPATLFINNKTGYRKFFEEQLSHQALKNEIMAIL"
     gene            complement(8430..9026)
                     /locus_tag="PLO70_05735"
     CDS             complement(8430..9026)
                     /locus_tag="PLO70_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQZ73994.1"
                     /translation="MTPERRDKLITVLSKRQPDLTVVLENVFDPHNISAVMRTCDAVG
                     IQEVYILNTKIPRHKKWGARSSSSAAKWLTVHQFENAPECFSSLRKRYSKILTTHLSS
                     DAVGLYQIDMTQPIALVFGNEHSGVSDEIRTLADGNFIIPQVGIIRSLNISVACAVTL
                     YEAFRQKNNAGHYNRKDMDHVMKQGLLEEWELKDNAEL"
     gene            9242..10699
                     /locus_tag="PLO70_05740"
     CDS             9242..10699
                     /locus_tag="PLO70_05740"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQZ73995.1"
                     /translation="MKYSPVQNYINGQFVNASSARTLPVISPIDGNQLSTVPMSTAKD
                     LDEAVKAARAAFPAWSKTPIKERVQVFFRYKYLLEKYLKELSELVQEENGKTYGEAVA
                     EVEKSIELTEFATSLPQLITGEILEVSKGVECRTEHVPLGVVASIVPFNFPAMVPNWT
                     IPNAIALGNCMIIKPSEKVPLSTARLAQLLKEAGLPDGVLNIVNGDSEIVNAICDHPG
                     IEAVSFVGSTKVAKIVYQRATQNYKRALALGGAKNHLMVLPDAIPGMTAQNVAASMSG
                     CAGQRCMAASAMIGVGNVDSIIDKICEEARKIIPGENLGAVISKESKERIERFIGEAE
                     RDGAKILVDGRNAKVKGKENGTYVGPTVIDYVTPQMSVAKEEIFGPVISIMRTNTVDE
                     ALAIENANPYGNAASVFTQNGGMARYIIDRASAGMIGVNVGVPVPREPFSFGGWNESK
                     FGVGDITGKSSIEFWTKLKKSTTKWNPESGVNWMS"
     gene            10783..12132
                     /locus_tag="PLO70_05745"
     CDS             10783..12132
                     /locus_tag="PLO70_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ73996.1"
                     /translation="MSETKVISESLDIIQDNLDYTLFSWSKQKGIAPIAVKHAEGVYL
                     YDYDGKRYIDFSSGLMNVNIGHGNQRVTDAVVKQMQEVAYVTPSCVTKVRGELGKKLA
                     DICPGNLNKAFFTLCGATSIENGIKLARLYTGRHKILTRYQSYHGASYGAMSASGDPR
                     RLPMDTQQAPNFVHFDLPYLYRWEYGEENLLKESVASLERVIAYEGPANIAAILLEGE
                     SGSSGCLKYPVGYLKAVREICNKHGILLIMDEVMSGFGRTGKWFGFENHGIIPDMIAM
                     AKGLTCGYLPFGCLMVSDTITAKYDDTVLSLGLTYSAHPVSCAAALETIKIYEDEGLI
                     ENCVAMGQYVNEQVELLKQKYPSIGDFRNTGLLGCIELVKNQKTKEPMAPFNAKPDEL
                     AVMNKVAAKIKELGMYTFVRWSYIFIAPPLCVTKEQIDEGLKIISEAIAIADQYYVE"
     gene            12202..13653
                     /locus_tag="PLO70_05750"
     CDS             12202..13653
                     /locus_tag="PLO70_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HQZ73997.1"
                     /translation="MNGNQSIYDNSLINEDLAPVPLEKRTWGTWNYAALWISMSLCIP
                     TYMLASSLIGGGMNWWQAILTIFLGNSIVLIPMILNGHAGAKYGIPFPVFARASFGTK
                     GANIPAMLRAIVACGWFGIQTWIGGDALYHILKAWNPSLADVDTQSLFPQALPMICFF
                     AFWFLNMYIVYLGVESIRKLLVFKAIFLPLAALALLAWAITAAKGLGPILEQPSKLHG
                     NDFWKYFFPALTGMVGFWATLSLNIPDFTRYAKSQKAQISGQAIGLPPSMTLFAFIGV
                     VVTSATPIVYGSTIWDPVQLAGKFDNKLLVSFAMLAVIISTLATNIAANIVSPANDFA
                     NLSPNKINFKTGGYITGIIGILIFPWKLVADPSGYIFTWLVGYSSLLGPVGGILIADY
                     YFIRKQQLAVDELYNSKGRYHFSNGFNTTAIIALLAGILPNIPGFLVTIKVIAADAVP
                     YWISNLYNYAWFVGFFVSGFVYWTLMKKRNYAI"
     gene            13722..15101
                     /gene="hydA"
                     /locus_tag="PLO70_05755"
     CDS             13722..15101
                     /gene="hydA"
                     /locus_tag="PLO70_05755"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HQZ73998.1"
                     /translation="MNILIKNGRIISATDDYVADIFIEGEIVKAIGKNLAVKAEKEID
                     AAGKLVFPGGIDPHVHLDMPFMGTFSSDNYETGTRAALFGGTTMVIDFILQKQGNSLR
                     SALEEWKSRSDNNCVGDYSFHMAVTDFNDDTKKEIQEMIEKEGITSFKTFMAYKGALM
                     IDDRQMIGLMQEVKKHGGLINVHATNGDMIDFLIAKHRAEGKLSPLYHYLSQPEVTEA
                     EASGRFADMANYTGCPGYIVHLTCEGALNAVRNATRRNQKVFVETCIQYLIIDASLYE
                     REDGAKWVMSPPLRQMKDQETLWAGINQGLVQVVATDHCPFMWEQKLMGKDDFSKIPN
                     GHPAIENRMELLYSEGVNKGKITLNKYVEVACTNPAKIFGMFPRKGTIAVGSDADIVI
                     FDPNEKHTLSAKTHHMNVDYSGYEGWEVTGKVKTVLLRGKIAIENNHCKIDRGYGKFI
                     RRNKVSQTI"
     gene            15179..16045
                     /locus_tag="PLO70_05760"
     CDS             15179..16045
                     /locus_tag="PLO70_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQZ73999.1"
                     /translation="MPRIIKSGLIQLSLPKTEGEGTIAEIKEAMVQKHIPLIEEAGKK
                     GVQILCFQEIFNTPYFCPGQDKAWYESAETVPGPTIERMQEYAKKYNMVMIVPVYEKE
                     QAGVLYNTASVIDADGTYLGKYRKNHIPHTSGFWEKFFFKPGNLGYPVFQTKYAKVGV
                     YICYDRHFPEGARALGLNGAEIVYNPSATVAGLSQYLWKLEQPAHAAANGYFMGCINR
                     VGTEKPWNLGKFYGSSYFVDPRGQIFAQASEDNDELLIADFDLDMIEQVRSTWQFFRD
                     RRPETYGKLTEL"
     gene            16111..17448
                     /locus_tag="PLO70_05765"
     CDS             16111..17448
                     /locus_tag="PLO70_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ74000.1"
                     /translation="MIKHNRLSSEQYAENFSDIHPPYENSTAAKVDANRCLFCYDAPC
                     MKSCPTSIDVPKFIKQIATENIKGSAKTIFSSNIMGAGCSKVCPVEKLCEGACVYNLL
                     EEEPIPIAKLQRYSTEMAMKENWQLFQRKTATGKKVAVVGAGPAGLSCAHVLSREGID
                     VTIYEKESKGGGLMTYGIAAYKVTPQFCEDEVNYITSIGGIEIKYNQELGKDITLSQL
                     KKEYDAVYLSIGVGVARQLEIPGEKLEGVVDAIEFIYDIRSKGYPSVPVGDKVAVIGM
                     GMTAIDAATQAKRLGAKEVTMLYRRTEAEMPCTKHELDIAMLDGCEVIWLAAPQKIKG
                     SAGKVKQLVCSKMKLGEPDASGRRSPVDTGETFTLDVDMIIKAAGQTPYEKLIKKLNI
                     QHSKFKITIENCATNIRGVFAGGDAVNGGKEVVDAVQAGKDGAAAILKFLKLC"
     gene            17528..17887
                     /locus_tag="PLO70_05770"
     CDS             17528..17887
                     /locus_tag="PLO70_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74001.1"
                     /translation="MKEDNKILNLTFDFSIQIIGLYKLLVQQNEYVISKQLLRSATSI
                     GANVEEATAAQTKKDFIMKMSIASKEARESKYWLRLLDKSQMVKADYSVYLTCIEHII
                     NILTKIVKTSQESLNSK"
     assembly_gap    17982..18081
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18116..19384
                     /gene="preA"
                     /locus_tag="PLO70_05775"
     CDS             18116..19384
                     /gene="preA"
                     /locus_tag="PLO70_05775"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HQZ74002.1"
                     /translation="MASIESTFLGIKSPNPYWLASAPPTDKKYNVLRAFEAGWGGVVW
                     KTLGSQVKNVSSRYSAVDFGGQRVMGFNNIELISDRPLDINLKEIAESVRLFPDRAMI
                     VSLMADNDKKSWHELIKKVEDTGAHGLELNFGCPHGMTERGMGAAVGQDPEIACMVVE
                     WVMETATLPVITKLTPNVHSVVPTGRSVVKAGTNALSLINTIQSVTGIDLDTLVPNPY
                     VAGKSVFGGYCGPAVKPIALKMLTTIAQDELTKKVPISGIGGISTWKDAVEFMLLGAS
                     NVQVCTAAMKHGFRIIEDLCEGLNNWMDEKGFATLNDFIGKSVPTITHWEDLDINYHH
                     IAKINQDKCIHCGLCYIACEDTSHQSINLMYGKPYNTYTIKEEECVGCNLCQLVCPVD
                     DCITMEEHRVAPEPINWKQWQERGLPLNDH"
     gene            19555..20439
                     /locus_tag="PLO70_05780"
     CDS             19555..20439
                     /locus_tag="PLO70_05780"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HQZ74003.1"
                     /translation="MGRKKTHDNLPSFDELIIPTLKALLELGGSGTIEEISAKVYEIE
                     KFSEEILQIPHGDDGGRSEIDYRLAWSRTYLKKYGLLENSSRGIWALAKTDFDITVLD
                     YNEIVRKVKEDEKQKQTIEKNHATKKEIEKEIIEEVETTEEWKEKLLNILYEITPAAF
                     ERLAQRILRESGFFQVEVTGKSGDGGIDGKGIVRVSGLLSFHVIFQCKRYKSSVTPSE
                     IRDFRGAMQGRADKGLFITTGKFTREALKEATRDGAPPIDLIDGELLCDKLKELKLGL
                     DTKLTETVEIKSDWFKNI"
     gene            complement(20645..21037)
                     /locus_tag="PLO70_05785"
     CDS             complement(20645..21037)
                     /locus_tag="PLO70_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HQZ74004.1"
                     /translation="MTEGYSNHFYNKNLQPNANRLRKEMTKAEACLWKFVLRAGKMKG
                     YGFRRQRPVLNYIADFMCKELMLIIEVDGSIHELEDVRKNDEQRQKVLEEAGFTVLRF
                     TNHEVLTNIQWVHSYLEEWIEKKNASKE"
     gene            21092..21664
                     /locus_tag="PLO70_05790"
     CDS             21092..21664
                     /locus_tag="PLO70_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQZ74005.1"
                     /translation="MRKIISFMHLSLDGFVAGPNGEMDWIKVDDEIFDHVGKRISEGD
                     TALYGRVTYQLMESYWPTAANKPNASKHDIEHSAWYSKVHKLVLSKTMKEADLPNTTI
                     ISENLSDSINKIRQQAGPDILLFGSPTATHSLMKLNLIDGYWLFVNPIILGRGIPLFA
                     DSKEKIKLNLVSTRPFTCGVTELNYTVDRQ"
     gene            21821..23182
                     /locus_tag="PLO70_05795"
     CDS             21821..23182
                     /locus_tag="PLO70_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQZ74006.1"
                     /translation="MNRSICRLAVSALLVLYLPASAQDQAKKKNIILIISDDHRFDAL
                     GIAGNTKISTPQLDALAVTGFYFPQATIHSPQCSPSRSTFLSGQPAHRHGVFSNDVLK
                     EAKDSSRFFQQPTLPQLLQSAGYHTFLSGKWHVPADPWKIGFEEIRYWMPGGAGAYKD
                     PQLAFGKSKEKKKTIGFTNEIFAKEIISFLESPAAKEKPFFVWFSATAPHAPYKPNPK
                     RIQNIYEGKTAKQLLPPAFPEQYTPGDFLHYYQAISFLDELVGRIIRTLDKTELSRET
                     IIVFIGDNGLMMGSRGMKGKVNPYEESVRVPLIIRMPQQNDTVHTITSPVSSLDISPT
                     LLALAGVKVPASWPGRDISGLLENGKKNQLDYAITEWADNEHGYAAYRLIRTKDYKLI
                     AWENLTKPMELYQLSNDPQETANLAGNADYKKIQQELLSQLKQWMSKTNDPALNWKNL
                     PGK"
     gene            23387..23707
                     /locus_tag="PLO70_05800"
     CDS             23387..23707
                     /locus_tag="PLO70_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQZ74007.1"
                     /translation="MRRDIFQAIADPTRRAIITLIALEAMTPNAIAEHFDSTRQAVSK
                     HLRILTECELVKQEYQGREIYYSLEIEKMKEIDKWLDQFRKIWETKFNQLDKLLATLK
                     KQKK"
     gene            23704..24201
                     /locus_tag="PLO70_05805"
     CDS             23704..24201
                     /locus_tag="PLO70_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQZ74008.1"
                     /translation="MNTNLLFDFVVDKTTKTVCITREFDADLSLVWDAFTIAELIDQW
                     IAPKPFTAKTKYQDFKVGGKRFYAMISPDGQERWVIQEYTSITPKTNFKMYNAFAGEN
                     EIPEPSGSEWDHTFSEQNEKTKVNISIYNESLERMERILDGFTQGMKLSLTNLENLLA
                     VLSGK"
     gene            24208..24639
                     /locus_tag="PLO70_05810"
     CDS             24208..24639
                     /locus_tag="PLO70_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQZ74009.1"
                     /translation="MNVKEQIKNYIAAQPEPKRSDMQELHKLIVNRLPTGKLWFLDGK
                     DSKGKIVSNPNIGYGSYTIHYTEGTTKEFYQVGLSANSSGISVYIMGIKDKTYLSRTY
                     GNKLGKATVTGYCIKFKTVKDIDIAILEDAIRYGFEGNMIS"
     gene            24707..26221
                     /locus_tag="PLO70_05815"
     CDS             24707..26221
                     /locus_tag="PLO70_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQZ74010.1"
                     /translation="MKPAKCIFFIAGLFLFPYKKLYSQSLSNFVSVNPTAQTSNFIFP
                     SATHRFQKIMEHGDVLPVGTMMDNFDFTGYMPIAGSSINGYLSINHEQTPGGVTAMNV
                     SFNSITKLWNKNGATAIDFSSVAGTARNCSGGLTPWGNIITCEEVISIADVNADGYND
                     LGWHVEINPSTKTVVRKLWAMGCGAKENIVIHSNRRTAYFGNDANPGYLYKFVATTMD
                     DLSAGSLYVYTGPKTGNGNWVLLNNTTQPDRNNTMVLGNAAGATVFNGIEDVEIGPDG
                     KIYFAVKGENRVYRFQDADPVSGTTTSAMETYVGNASYNITHAGGTVSTPWGTGNDNL
                     AFDELGNLWVLQDGGNNYIWVVMSNHTQAVPNVKLFGIAPAGAEPTGITFTPDYKFLF
                     ISFQHPTATNNSDLQMDAAGNSIGFEKDIAIAIGVNSELGCITGDGCFVATNTNNVGV
                     GTDNPKAKLQVRNGDVSLETIGTGIILKSPNGNCWKITISNTGVITSVAVPCLE"
     gene            26402..26752
                     /locus_tag="PLO70_05820"
     CDS             26402..26752
                     /locus_tag="PLO70_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HQZ74011.1"
                     /translation="MDNHRVFKMSFAGVYPHYIQKAEKKGHTKEELDTIICWLTGYTQ
                     KQLQQQIDNKIDFETFFAQAPQLNPNVSKITGVICGYRVEEIEDKLMQKIRYLDKLVD
                     ELAKGKAMEKILRK"
     gene            26870..29938
                     /locus_tag="PLO70_05825"
     CDS             26870..29938
                     /locus_tag="PLO70_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQZ74012.1"
                     /translation="MNKKLKKIVKITGFTLLVLIAAAFLIPLLFKKQITELVKKEINN
                     NLIAKVDFKDVSLSLFRHFPKISILLEDLSVVGTNEFDSDTLLSAKTLDASVNLISAI
                     RGKNIKVYGVYVQSPRIHALINKEGKANWDIAKESTDSSGKADNSPSEFKMNLQQYEI
                     SDGYVYYKDESIDLSAEISGLEHKGSGDFTEDVFTLSTKTKATSTDFTYAAVPYLVNA
                     QTDIDGDIQIDNTNSKYSFKTEAITVNKLKLNADGFFRFVNDSTYDMDIKFKTPSNDF
                     KDILSLIPGIYKQDFATIKTSGNAAFNGFVKGIYSPQQLPAYDVNLEVKDGFFQYPDL
                     PKPVKNIQLAMHVSNPDGKKDNTVVDISKGHVEMDNEPFDFKLLFRNPETAQYIDAVA
                     KGKLDLANLSKFIKLEAGTKLAGLVWADLFAKGNMSALENQQGNFTAGGFLDIKNLFY
                     SSAAFPQPLENGNMKVQVENSGGIADNTSINITTGHMEVGKDPVDFNLQLRHPMSFVD
                     FTGAAKGRFTLNHIKQFVTLDPGTSISGILNADLGFSGNKTAIDKKEYDKIIINGTAG
                     LNNLNYVSKDYPTGLSITNSALSFQQKNVTLNNLAGNYLNTNFSANGTFNNLVGYAMQ
                     DQPLAGTLNVTADKMNLNDWMGTDTTTTTTSAAASADPFAVPANMNLTINAKAGKVKY
                     DKVDYTNITGTLLLKDETVKLQNVKTEALDGTIVFDGSYSTKNNKKDPAISMTYEVKN
                     IDVQKAFFSFNTIQKLMPVGQFLAGKLNSQLTMNGNLDGNMMPDLSTLNGKGNLLLIE
                     GILKKFAPLEKIASTLQIDELKSISIKDVKNYIEFAKGKVLVKPFSVKVKDIEMLIGG
                     MHGFDQSIDYVVQMKVPRKYLGNEGNNILNNLVTQAVNKGIPVKLGETVDLSFKMGGT
                     LANPILRTELKEVAGDAVKEMQQLAVDFAKAKLDTAKQTIKDSLTGVKNQVLNDLKKE
                     ALDRMSGNKDSVKTNPADSSKKKSEQTIRNTINTLFNKKKKPASDTAVKN"
     gene            30072..30635
                     /locus_tag="PLO70_05830"
     CDS             30072..30635
                     /locus_tag="PLO70_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HQZ74013.1"
                     /translation="MSTKKKLSAAQSQELLSTLKTRFEKYIDRHKGIEWAKVQSKLEA
                     NPDKLWSLHEMETTGGEPDVVGYDKKTGEYFFYDCSAESPADRRSLCYDAAALASRKE
                     NKPKHSAMGMAVEMGIELLSEEEYRALQQFGEFDLKTSSWIKTPDNIRKLGGALFCDR
                     RYATVFTYHNGAESYYAARGFRGSLRV"
BASE COUNT         9661 a         6161 c         6585 g         8130 t
ORIGIN      
        1 accgcttatt caatgtatcg gaaaacgaga tcaattgtac cgaagcccct tccaatgctt
       61 tattcttact atccattaca ttgccggtaa ggatgccgga aatagtttta tcctggcccg
      121 tagcactaaa taccaataaa atgctggaaa tggtaaaaag gatgattttt ttcaacattg
      181 acctttagac gccaaatata cctcatacct gttgatggtt tcaggctaat ttacagcaat
      241 ggtaagggca aacgtttgtg attcaacctt tcagctaatt atgacatact gtcattatct
      301 gaccggtttt gggtatattt gctgtccaaa aaatttgttt gaatgcttga tttgatgaca
      361 gataaggtgc aggatgaaac aaggcagggt gaggcctttg ccccctttgc caatgatcca
      421 aatcagtata agaagcgatt ctatattgaa agctacggct gccagatgaa tttcgcagat
      481 agtgaagtcg ttgcctctat cctgaataaa gaagggttcg gggccacccg gaatgtggag
      541 gaagccgacc ttatatttct gaatacctgt tccatccgtg aaaaagcgga gcaaaccgtt
      601 cgtaaacgcc tcaccgaatt cagaaaactg aaagaaaaca gaaaaggaat gctggtgggt
      661 gtgctgggtt gtatggcaga gcggttaaaa tcaaaattcc tggaagaaga gaaactggtt
      721 gatattgttg tgggccccga tgcctaccgg tcattgcctg aattggtaat tgaagcatcc
      781 ggcggacaaa aagcggtaaa tgtgctgctg agccgtgatg agacctatgc agatatttca
      841 ccggtgagac tggacagtaa tggtgttagt gcatttgtca gcattatgcg gggatgcaat
      901 aatatgtgct ccttctgcgt ggtgccattc accaggggca gggaaagaag ccgggatttt
      961 gaaagtatca ttcacgaatg caaggactta ttccaggctg gttataaaga agtaacatta
     1021 ttggggcaga atgttgatag ctattatttc gtcgatgaag caaaaaaaga aacgatcact
     1081 tttgccaggt tgcttgaaca ggttgcttta atatctcccc tgctccgtgt tcgtttttcc
     1141 acttcacatc ccaaagacat aactgatgag gtattacata cgatggcgaa gtatgaaaat
     1201 atctgcaact atattcactt accggtacaa agcggttcct ccaggatctt acaactgatg
     1261 aaccgcacct atactagaga atggtatatg gcaaaagtgg atcgtatccg ccagctcatt
     1321 cctgattgtg gtatatcatc agatttcatt gccggtttct gtacggaaga agaaaaagat
     1381 caccaggata cactgagtat aatggagtat agccgttacg atttcagtta tatgtttttc
     1441 tacagcgaaa ggcccggcac cctggcacat agaagataca aagacgatat tccggaggaa
     1501 gtgaaaaaaa gaaggttaca ggaaatagtg gaagtgcaaa acaggttgtc gctggaaagc
     1561 aataaaaaag atataggcaa aacattcaaa gtgctgatag aaggcgacag caaaaaaaat
     1621 gaaaaggaat ggaaaggaag aaacagccag aataaagtga ttgttttccc caaaggaaac
     1681 catgacctga agaaaggaga ttatgtaatg gtgaaagtga atgagtgcac acaagcaaca
     1741 ttactgggag aaattattaa ataacataaa aatctgtcgg ctacagcccc ggtaataacc
     1801 gggtgccgat tgaagaagat atggagattc aaattataaa aaacagattt ggcatcattg
     1861 gcaactcccc tgccctcaat tatgcattgc aggtggcaat acaggttgcc aatactgatc
     1921 ttaccgtttt aattaatggc gaaagcggcg ttggtaaaga agttttctcg cagatcatcc
     1981 actctctttc tgccaggaaa cacaaccctt ttatagccgt aaactgcggc gctattccgg
     2041 aaggaacgat cgattcggaa ttattcgggc atgagaaagg ttcattcacc ggtgcggtgg
     2101 atgcaagaaa aggatatttt gaaacggtaa acggcggtac gatcttcctg gacgaaattg
     2161 gtgaactacc attaggtacc caggcaaggt tactgagagt gttagaagca ggagaatata
     2221 tccgtgtggg ttcatccaaa gtacaaaaga cggatgttcg tgtaattgca gccaccaata
     2281 aagacctgat gcagttagta cagtccggaa ggttcaggga agatctttac taccggctca
     2341 gcacggtgcc tatccgtgtg cctgcactac atgacagacc agaagacatt catttgctct
     2401 ttagaaaatt tgcagcagat tttgccaata aatacaaaac atcacccgtg caactggatg
     2461 atgaagcaaa acaactgtta ataaactacc cctggccggg gaatgtccgt gaattaaaga
     2521 atattgcaga acaaatttca gttctttcac aggacaaaat gataacggca aaagaattca
     2581 ataaattcct tcaaccctat tccaataaca ggttgccggt gctggcttca cccggcggac
     2641 acactgctga attttccaat gaaagagaga ttctgtacaa actctttttt gatatgaaaa
     2701 aagatgttac agaattaaaa agaatgtttc tggatgtatt acaaaacccg gaaatggtag
     2761 cccagaatca gcatttcatt aatgagctga aagaattgag aagccaggga caggaggtga
     2821 tacaacccat gcctttatcg atcagcactc aacctgtcac gcaaacggta cagccggtta
     2881 tgatgcgtaa tgatattcat gaccatgtag aagtagagga aagcctgaat ataatggata
     2941 aagaaaaaga acttatcatt aaggcattga aaaaacataa aagcaaacga aaagacgcag
     3001 cccttgacct gggcatcagc gaaaggacct tatacaggaa attaaaagaa tacgacatcg
     3061 aagaataatg aatacaccta aaaaaatatt tttacttgct gtcattggcc ttttcagctt
     3121 catggcgttg ttcagcaaca gcagttgcgg catttataaa ttcaatgacg cttctgttcc
     3181 ggatagtatc aaaaccgtta aagtcaactt tattgaaaat aaagcaacct atatcaatcc
     3241 gcagctgagc ccgagactga cagataaact acgtcagaaa attgttggcc agacaaagct
     3301 tacacaaacc aataacgaca atgtggattg ggaaatcagc ggaacagtca cccaatacag
     3361 cttcagtact tctgccatca ccgggcaaca ggctgccaat aacaggctaa cggtgggcat
     3421 acaaatgatt ttagttgacc ataaggctga taaaacagaa aaatttgatg tcagcaggag
     3481 ttttgaattc aagggcaatc aatcttttca gcaggctgaa aacgacctgg cagaagaaat
     3541 gataagaaca cttactgacg acattttcaa taagcttttt tctaaatggt agaccagtaa
     3601 aaaccaggtt ttaatcaatt ttacacctct ttttacttaa cttgttaata tgaatgtccg
     3661 acttaatcaa ttggtaaaat cactgctgca aaaagaatcg ctggaccaat gcagcctgcc
     3721 agagttgaca gcttttgctg agaagaatcc ttattttggt gctgcacaat tgttacttac
     3781 caaaaaagta caaacagaac agccggaaaa atatgatgag caattacaga aaacattact
     3841 tttttttcat aaccccttat gggtagaaca tttgctgaat gataccggcc tggctgaaat
     3901 aagcaagtca aaaaaagaag aagaaaccgt ttcacagcaa ccagaggaaa gtacagctat
     3961 cgcaccagca tcgttacaag attcaatctg gaccgacgaa tcaatacctg taattgttgc
     4021 taccccgcaa actgaagcca caatagctga ggctagatct tctgttgcgg aagagaatcc
     4081 ggcaattgtt ccttccatac aaattgagaa tgctgcaagt acggaaccag cgctaacatt
     4141 tgagcccttc tttacggtag attattttgc ttcacagggt gtcaagttca aggaagaaga
     4201 aaaaccggtg gataaattcg gtcagcaatt aaaaagtttt acagactggc tgaaagcaat
     4261 gaaacggtta cctgtgactg aaatggctaa atccgtagaa acgcaggctg aacaaaaagt
     4321 agaacaactg gctggacaat ccattgcaga cagggaagta gtaacagaag caatggccga
     4381 agtatgggaa aagcagggaa atgtggccaa agccattgag ttatacagca aattaagttt
     4441 gctcgaaccc tctaaaagcc cttattttgc agcgaaaatt gaagatttaa agaaaataaa
     4501 ctgatatgac gactctattt gtgatattga ttattctagc ctctgttgta cttggcctga
     4561 tcgtgttagt gcagaaccca aaaggtggtg gcctggccgg aaatattgcc ggtttcagca
     4621 accagtttat gggagttaaa caaactaccg atgtgctgga aagaggtacc tggatatttg
     4681 gaggtgttat tgctgtttta tgcctcttgt ctgttctgtt tatctctaaa tccggcccca
     4741 gttccggacc tgacagaact aaagatgcga ccggggcttc tgttcctgct acacaaccgc
     4801 agcctaataa ccagaacaca ttgcagctaa atccgcaaac aagtcctgtt actccagatt
     4861 ctactaaata ataagtaatt ataatattaa tttaaccccg gtctcatttg aaaagagatc
     4921 ggggtttttt aattaatttg agcatcgggt atgagcaaga aaaaaattat taccggagtc
     4981 attctaatcc ttcttattgt cgcggcattt atcgggtaca aaatagcagg ccctgctgtt
     5041 tccgcaaaaa aacaaaccta cttctatata aaaacaggcg aatccctgga aacagtgaaa
     5101 caaaacctgg tcagtcaaaa aataattacg ggtaaagcct ggtttaatcg ggtagccggc
     5161 tggctgaaat atactgttgt aaaaccgggg cggtatgaaa ttaaagacgg gatgagtctg
     5221 cttacattgg caaaaatgct ccggaatgga aaacaagcac ctgtaaaaat ggttcttgta
     5281 aaagagagaa ccaaagaact ctttgccgga aagatgggaa agaaatttga tactgaatgt
     5341 gattcattac agatgatcaa ttttctcaat aacaatgatt cgttaaaaag atttgggatt
     5401 gatacctcta cagtgatggc cattgtaatg ccgtatacct atgaaataac ctggaacagc
     5461 agtcccggta agatcgtaca gcagttttac acagcctata aaaaattctg gtcacaagaa
     5521 cgaaagttga aggcagacag cctgcgcctt actcctttgc aaatatccgt attggcgtcg
     5581 attgtagaag aagaaaccaa taagaaagca gataaatata atattgccag cacttatctt
     5641 aaccggctta aaatcggtat gaaactacag gccgatccaa ccgtaaaatt tgtaacccgg
     5701 aattttcaat tgggaagaat aaccggtgtt catcttaaat tagaatcgcc ttataataca
     5761 tatttaaata ctggcctccc acccggcccg atctgcactc cttctgttga atcaattgaa
     5821 tctgtattga acgcaccaaa aactgattac ctttttttcg tagccagtta caaatttgac
     5881 gggagcacta tcttcaccag taattataca gatcatacaa aatatgttaa actttttcat
     5941 gccgaacaga acaggaggtc cgattcctta aagaaattga aggttaaata aaaatgccgc
     6001 ttttaaaaaa aatattaacc agcattgaag cttctgctcc tgttgctttt attatcaaaa
     6061 aaagcaagtc cacttccctg cccggattca acggcatccc gctgtttgat gtgatcaatt
     6121 tttttatcgg gcaggtaaaa acaatcggga tgacagaaag agcctctgca attgccttta
     6181 actttgttat ggcgatccca ccagctatta tcttcctctt tacattaatc ccctttatac
     6241 ccatcacaga ttcatttcag caggaaatgt acagcctgat aagagatgtg atacccgggg
     6301 aaaaggataa tgcagtattg ataaagttcc tggaagattt tattaataat ccccgtaccg
     6361 gcttattgtc attcggtttc ttactttcta tgttcttttc ttccaatgcc atgatgggca
     6421 tcatgcgttc atttgacaaa aattatattg gtttcagaaa aagaaaggaa tttcaaaaaa
     6481 ggatggttgc gataaaaata accatgctgc tgtacctgtt tgtttttgct tccgtattac
     6541 tgcttgttgc ccgtggcgct gtactagaat ggctgggctt aacaaataaa acagcgattt
     6601 ttataatcaa taatctccgc tggatagtga taatcctttt attttttgcc tgtatctctt
     6661 ttatctaccg ccatgctcct tccgttcaca aaaaatggcg gcttattaac ccgggttcaa
     6721 tactggctac tttcctgatg ctgttgatga cttttgctat ctcctggtgg gtatcccatt
     6781 ttggtaatta taaccagatt tatggttcaa tcggtaccat attaattatc atggcactga
     6841 tctttattaa ctcccttgta ctgctgattg gctttgaact caatgtcagc atttcctccc
     6901 tgaaaaaaat tgcagacgaa cggaaagata gtatggcagg cggtgaagga atatccgcct
     6961 gaattaccga aatttatagt cctaaaaata ttgactatga aaaaaatatt gttttcaata
     7021 ctgccggttg ctgccttagg gcttgttact ttaagctcag taagtgaccc cttacctata
     7081 ggagctgcag cgccaaatgc ggatctgaaa atgaaagata tttctggcaa agaagtatca
     7141 ctgaatgatg caaaaaagaa aaatggactg ctggtgatgt ttagttgcaa tacttgtccc
     7201 tatgttatca agaaccagtc ccgtaccaat gaaatagcca aatatgcatt ggataaagag
     7261 attggtattg caatattgaa tcccaacgaa gcttaccggg atgaccagga ttcttatgaa
     7321 gccatgaaac agtatgcggt tgaccagggt tatgcctggt attacctggt tgataaaaac
     7381 catacaatgg ctgatgcatt tggcgctaac cgtacgccgg aatgtttcct tttcgataaa
     7441 aacgggaagc ttgtatatca tggagctatt gacgatagcc catcggatgc aagtgcggta
     7501 agccgtaaac acctgaaaga agcaattaat gaaatcacga ccggaaaaga tgtctccgtt
     7561 aagcaatcaa aatcggttgg ttgtactatt aagaggctta aataaaagcg caaaatctta
     7621 ttcagacctg ttcccctcac ggcgaacagg tttttttttg aaattattat ggaaatcccg
     7681 gcaggatgca tcaaagtgat aattccgcat tcataaacca aaaactttgc ttatgctatc
     7741 ccgccaaaca ctcaaccgga tcatcattat cggctttatg gttttagtag gctttgccct
     7801 tgccaaagca atttatcata acagtgtatt agggatcatt atggcactga caagtcttgg
     7861 tgccggtatc tattttctgt atctaatggc aaaggccaaa gaagagatgg aggccgggga
     7921 tgcatcccgt taattcattc agagtatagc catgatttca tttttcagtg cctggtgaga
     7981 gagctgttct tcaaaaaatt tccggtaacc tgttttatta ttgataaaca gtgttgccgg
     8041 aattgctcct gaccatttgg tatcaacttt aggacagaag taatcagcat tcgtttcgtc
     8101 cagccataaa atgggggcgg tgatctttct ttttgcagca aaagattcaa ctcctttggg
     8161 aaataattct ttaaagtcaa gacttacaag gagcagttgt acactatctt tttcatgatt
     8221 ttttgcctct tctataaaat atggaatctc ttccacgcaa ggcatacacc aggtagccca
     8281 gaaattgacg atcagcggtg ttttactttc cgcaatgatc ttttccagtt ctgtgatctt
     8341 tattgatctg actggctgcg cctgcagcat tccggcaaca atagtaaaga tggtaagtaa
     8401 gtatagcttt ttcattctta attcttaact cataactctg cattatcttt caactcccac
     8461 tcctccagca atccctgctt catgacgtgg tccatatctt tccggttgta atgtcctgca
     8521 ttattctttt gccggaacgc ttcatacaat gttaccgcac aggccacact gatattcagt
     8581 gaccggataa ttcctacctg cggaataata aagtttccat ccgccaatgt cctgatctca
     8641 tcactcaccc cgctgtgttc attgccaaag accaatgcaa ttggctgtgt catgtctatc
     8701 tggtataaac ctacagcatc actgcttaag tgggttgtta aaatttttga ataacgtttt
     8761 ctaagtgatg aaaaacattc cggggcattt tcaaactggt gaacggtcag ccacttggct
     8821 gcactggacg aactcctcgc tccccatttt ttatgccttg gtattttggt attgagaata
     8881 tatacttcct ggataccaac agcatcacag gtacgcatta ccgccgaaat attatgcgga
     8941 tcaaaaacat tttccagcac aacggtcaga tcaggttgtc gtttcgacaa taccgtaatc
     9001 aatttatctc ttcgttccgg tgtcattgaa attgaagttg ttagcttgta ataagcggca
     9061 aattaagtat atttataatt gatggaatac ataaatacat tttgaaacac aaagactaca
     9121 agacaaggag gcaaggggaa gaaaccaatt tctttaccaa gtctttcctt tgtgtctttg
     9181 ttcctccgtg tctctgtgtt tctaacctcc ttttggaact aattaaaatg aaatttacaa
     9241 tatgaaatat tctcctgttc agaattatat caacggacaa tttgtaaatg cttcttcagc
     9301 ccggacattg cctgtcattt ctcccattga tggcaaccag ttatcaactg ttcccatgtc
     9361 aactgcgaag gatctggatg aagcagtgaa agcagccagg gctgcttttc cggcctggag
     9421 caaaaccccg ataaaagaaa gagtgcaggt ctttttccgg tataaatatt tattggaaaa
     9481 atatttaaaa gaattgtctg aactggtgca ggaggagaat gggaaaacct acggtgaagc
     9541 agtggccgaa gtggaaaaga gtattgaact aactgagttt gctacttccc tgccgcaatt
     9601 aattacgggt gagatactgg aagtaagcaa aggtgttgaa tgccggacag aacatgtacc
     9661 gctgggagtc gttgcatcaa tcgttccttt taacttcccg gcgatggtac cgaactggac
     9721 gataccgaat gccattgcac tcggcaactg catgatcata aaaccatcag agaaagtacc
     9781 actgagcacg gcaagacttg cacaactatt aaaagaagcc ggcttgccag atggtgtgtt
     9841 gaatattgta aatggcgaca gcgagattgt caacgccatc tgcgatcatc cgggcattga
     9901 agcagtttcc ttcgtcggct ctaccaaagt tgcgaagatc gtttaccaga gagctactca
     9961 aaattataaa cgggcattgg cattgggcgg ggcaaaaaat catctgatgg tattacccga
    10021 cgccattccg ggtatgacgg cgcagaatgt ggctgcatca atgagtggtt gcgccggaca
    10081 acgttgcatg gccgcatctg ctatgatcgg tgtgggcaat gtagatagca tcattgataa
    10141 aatttgtgaa gaagcgagga aaattattcc cggggaaaat ctcggtgctg ttatcagcaa
    10201 agaaagcaaa gaacggattg aaagatttat cggcgaagca gaaagagatg gtgcaaaaat
    10261 tttagtggat ggccgcaatg caaaagtaaa agggaaagag aatggcacct atgttggtcc
    10321 gacagtgatt gattatgtaa ctccacaaat gagtgtagcc aaagaagaga tcttcgggcc
    10381 ggttatttct attatgcgta ccaacactgt tgatgaagca ttggctattg aaaacgctaa
    10441 cccttatggc aatgcagctt cggtatttac acaaaatggt ggcatggccc gttatatcat
    10501 tgatcgtgcc agtgccggta tgatcggcgt gaatgtgggt gtgcccgtac caagagaacc
    10561 gttctccttc ggtgggtgga atgaaagtaa gtttggtgtg ggtgatatca ccggaaaaag
    10621 ttcgattgaa ttttggacaa agttgaaaaa gagtactacg aaatggaatc ctgaatctgg
    10681 agtgaactgg atgagttaat aaattattat aaatgttgaa ttttaaatgt tgaatgaaaa
    10741 atgcatttaa aattcaacat tcaacattta aaataaataa aagtgtctga aactaaagtt
    10801 atctccgaat cattagacat catccaggac aatcttgact acactttatt ctcctggagt
    10861 aaacaaaaag gtatcgcccc gattgctgtt aaacatgccg aaggtgtgta cctctatgat
    10921 tacgatggca aacgttatat tgatttctcc tccgggctga tgaatgtgaa tattggtcat
    10981 ggtaaccaac gggtgacgga tgcggtggta aaacaaatgc aggaagtggc ctatgtaacg
    11041 ccgagttgtg taaccaaagt gcggggagaa ttgggaaaga agttagctga tatctgtccc
    11101 ggtaatttga ataaagcatt ctttacgtta tgtggcgcta cgtccattga aaatggaatc
    11161 aaattggcaa gactttatac cggccgtcat aaaatattga cccgttatca atcttatcat
    11221 ggtgcatcct acggtgctat gagtgcaagt ggtgacccac gccgtttacc catggataca
    11281 cagcaggctc cgaattttgt gcattttgat ctgccttatt tataccgctg ggaatatggc
    11341 gaagaaaatt tattgaaaga gtccgttgca tcattggaaa gagtgatcgc ttatgaaggc
    11401 ccggccaata ttgctgctat tttattagaa ggtgaatccg gttcatctgg ctgtttgaaa
    11461 tatcctgttg gttatttaaa agcagtaaga gaaatatgca acaagcatgg tatactactg
    11521 atcatggatg aagtgatgag tggtttcggc agaacgggta aatggtttgg ttttgaaaat
    11581 catggcatca ttccggatat gatagctatg gcaaagggac ttacctgtgg ttacttacct
    11641 tttggttgtt tgatggtgag tgatacgatc acagctaaat atgatgatac tgttttgtca
    11701 ttaggactca cctactctgc ccacccggtt agttgtgctg ctgcattgga gacaattaaa
    11761 atttatgaag atgagggact gatcgaaaat tgtgtggcta tggggcaata tgtaaatgaa
    11821 caagtggaat tattaaaaca aaaatatcca agcataggcg atttcagaaa taccggtttg
    11881 ctgggttgta tagaattggt gaagaaccag aaaaccaaag agcccatggc tccttttaat
    11941 gcaaagcccg atgaactggc cgtgatgaat aaagtggccg caaagatcaa agagctggga
    12001 atgtacactt ttgtacgctg gagttatata ttcattgcac caccgctatg tgttacgaaa
    12061 gaacaaattg atgagggatt gaagattatt agtgaggcaa ttgcgatagc agatcagtat
    12121 tatgttgaat gataaatttt gaatgttaaa tgaatggctg tttttcattc agcatttaac
    12181 attcaacatt caaaataaac catgaacgga aatcaaagca tatacgacaa ctccctaatc
    12241 aacgaagacc tcgctcctgt tcccttagaa aaaagaacct ggggcacctg gaactatgcg
    12301 gccttatgga taagtatgag tttatgtatc cccacgtata tgctggccag ttcgttgatc
    12361 ggtggaggca tgaactggtg gcaggctatt ctcaccatct ttcttggcaa cagtattgtg
    12421 ttgataccga tgatactgaa cgggcatgcc ggagcgaaat atggtatccc ctttcctgtt
    12481 tttgcaagag caagttttgg aaccaaaggc gctaatatcc cggcgatgct gcgggctatt
    12541 gtggcctgtg gctggtttgg tattcaaaca tggattggtg gcgatgcatt atatcatatt
    12601 ctcaaagcat ggaatccatc actggcagat gtagatacac agtcattatt tccgcaggca
    12661 ttaccgatga tctgcttctt cgctttctgg ttcttgaata tgtacattgt ttatctcggc
    12721 gtggaaagca tccggaaatt attagtattc aaagcgatct ttttaccgtt ggcggcattg
    12781 gccttattag cctgggccat cacagccgca aaaggattag gaccgatatt ggaacaacct
    12841 tccaaactgc acggcaatga tttctggaaa tatttctttc cggcacttac gggaatggtt
    12901 ggtttttggg caacactttc tttaaatatt cccgatttta cgagatatgc caaatcacaa
    12961 aaagcacaga tcagcggaca ggcaattggt cttccgccat caatgacact ttttgctttc
    13021 attggtgttg tggttacgtc tgcaactcct attgtgtacg gcagcactat ctgggatccg
    13081 gtacaactgg ccggtaaatt tgataataaa ctcctggtaa gttttgctat gctggcggtg
    13141 atcatctcca cgctggcaac aaatattgct gccaatatcg tcagcccggc caatgacttt
    13201 gctaacctgt cgccgaataa aataaatttt aagaccggcg gttatatcac cggtattatt
    13261 ggtatcctga tctttccctg gaaattagtt gccgatccca gtggttatat ttttacctgg
    13321 ctggtgggtt actccagttt gctgggccca gtcggaggaa tcctgattgc tgattattat
    13381 tttatacgca aacaacaact ggcggtagat gaattgtaca attcaaaagg acgttatcat
    13441 ttcagtaacg gctttaatac aactgcgatc attgcattac tcgctggtat attacccaat
    13501 atccctggtt ttttggtaac aatcaaagta atagctgctg atgcagtacc atactggata
    13561 agcaatctct ataattatgc ctggtttgtt ggcttctttg taagtggctt tgtatattgg
    13621 acattaatga aaaaaaggaa ttatgctatt taataaattt tgaatgttaa atgttattga
    13681 ctcattcaac attcatcatt caacatttaa aataatctcc catgaatatt ttaataaaaa
    13741 atggaaggat catctcagca actgatgatt atgtagccga tatttttatc gaaggcgaaa
    13801 tcgtgaaagc tatcgggaaa aatttagccg taaaagcaga aaaagaaata gatgctgctg
    13861 gtaaattagt ttttccgggt ggtatagatc cccatgtgca ccttgatatg cccttcatgg
    13921 gaactttctc ttcggacaat tatgaaacag gaacaagggc ggcgttgttc ggcggcacca
    13981 cgatggtgat cgattttatt ttacagaaac agggaaactc ccttcgctct gctttggaag
    14041 aatggaaaag ccggagtgat aacaattgcg tgggggatta cagtttccac atggcggtca
    14101 ctgatttcaa tgacgataca aaaaaagaaa tacaggaaat gatcgagaaa gaaggcatca
    14161 cttctttcaa aacatttatg gcgtataaag gcgccttaat gatcgacgac cgccagatga
    14221 tcggcctgat gcaggaagta aagaaacatg gcggcctgat caatgtacat gcgaccaatg
    14281 gggatatgat cgacttcctc attgccaaac accgggccga ggggaaactc tcaccgcttt
    14341 atcattattt atcccagccc gaagtaactg aagcagaagc cagcggacgg tttgcagata
    14401 tggcgaacta tacaggctgt cccggttata tagttcacct gacttgtgaa ggggcattga
    14461 atgcggtaag aaatgctacc agaagaaacc agaaagtatt tgtagaaacc tgtattcaat
    14521 atctcatcat tgatgcttct ttatacgaaa gagaagacgg tgccaaatgg gtgatgagtc
    14581 ctccccttcg tcaaatgaaa gaccaggaaa ccttgtgggc cggcatcaac cagggattgg
    14641 tgcaggtagt cgccactgat cattgtcctt ttatgtggga acaaaaactg atgggcaaag
    14701 atgatttttc taaaattccc aatggtcacc cggccattga gaaccggatg gaactattat
    14761 acagtgaagg cgtgaataaa ggaaagatca ccctgaacaa atatgtggaa gtggcttgta
    14821 ctaatcctgc aaaaatattt ggcatgtttc ctcgtaaagg aacgatcgct gtaggtagcg
    14881 atgcagacat cgtcatcttc gatcctaatg aaaagcatac cctttctgcc aaaactcatc
    14941 acatgaatgt ggattactca ggctacgaag gatgggaagt aacgggaaaa gtgaaaactg
    15001 tattactgag aggaaaaata gcgattgaaa acaaccattg caaaattgac agaggttatg
    15061 gtaaatttat aaggagaaat aaagtctcgc agacgatata atttatttta aatgttgaat
    15121 gataaatttt aaatggcccc attcaacatt caaaatttaa cattcaaaat aatcacccat
    15181 gccaagaatc atcaaatcag gtcttattca gcttagtctt ccaaaaacag aaggcgaagg
    15241 aacaattgca gaaattaaag aagcaatggt gcagaaacat attcccctga tcgaggaagc
    15301 cgggaaaaaa ggagtgcaga tattgtgctt ccaggaaatc tttaatactc cctacttctg
    15361 ccccggccag gataaagcct ggtatgaaag tgcagaaacg gttcccggcc ctactattga
    15421 acggatgcag gaatatgcaa agaagtataa tatggtgatg atcgttccgg tgtatgaaaa
    15481 ggaacaggcc ggtgtattat ataatacagc ttctgttatt gatgcggatg gaacatatct
    15541 cggcaaatac cgcaagaatc atattccgca tacgagtggt ttctgggaaa agtttttctt
    15601 taaaccgggc aaccttggtt atcccgtttt ccagaccaag tatgcaaaag tgggtgtgta
    15661 catctgttac gaccgtcact ttccggaagg tgcaagggca ttaggcttga atggtgcaga
    15721 aatagtttat aatccgtcag caacagttgc gggtctatcg cagtatttat ggaaactgga
    15781 gcaaccggcg catgccgccg ccaatggtta ttttatgggc tgcatcaatc gtgtcggtac
    15841 agaaaaaccc tggaacctcg gtaagtttta tggcagcagt tattttgttg atccaagagg
    15901 acaaatattt gcgcaggcat cagaagacaa tgacgaatta ctgatcgcag attttgattt
    15961 ggatatgatc gaacaggtaa ggtctacatg gcaatttttc agggacagaa gaccggagac
    16021 atacggaaag cttaccgaat tatgatgaat gttgtatgat aaatgctaaa tggtttttca
    16081 ttcaacattc aaaatttaac attcaacata atgataaagc acaacagatt atcgagtgag
    16141 caatatgctg aaaatttcag cgacattcac ccgccgtatg aaaatagtac agcggccaaa
    16201 gtggatgcca accgttgttt gttttgttat gatgctccct gcatgaagag ctgccctacc
    16261 agtattgatg tgcccaaatt catcaagcag atcgctacag aaaatataaa aggttctgcc
    16321 aaaaccattt tctcctctaa tatcatggga gcaggttgta gtaaagtttg tcccgtagaa
    16381 aaattatgcg aaggggcctg tgtatataat ttactggaag aagaaccgat tcccattgcc
    16441 aaattgcagc gctatagtac cgaaatggcg atgaaagaaa actggcaatt gtttcaacgc
    16501 aaaaccgcaa ctggtaaaaa agtggctgta gttggtgccg gacctgcagg cttaagctgt
    16561 gcccatgtat tatcaagaga aggaattgat gtaacgatct atgaaaaaga aagcaagggc
    16621 ggcggactga tgacctatgg cattgcagcc tataaagtaa ccccgcagtt ctgcgaagat
    16681 gaagtcaact atatcacttc cattggtggt attgaaataa aatacaacca ggaactggga
    16741 aaggatatta ctttatcaca actgaaaaag gaatatgatg ctgtttacct cagcatcggt
    16801 gtgggtgtgg caaggcaatt agaaattccg ggagagaaac tggaaggtgt tgttgatgca
    16861 attgaattta tttatgatat caggagcaaa ggttatccat ccgttccggt tggcgataag
    16921 gtagccgtga tcggaatggg catgaccgct attgacgccg ctacacaggc caaacgtctc
    16981 ggtgcaaaag aagtaacgat gctctaccgc cgcaccgaag cggaaatgcc ctgcaccaaa
    17041 catgaactgg atattgccat gctggatggc tgtgaagtga tctggctggc agcgccgcaa
    17101 aaaataaaag gcagtgcagg aaaagtaaaa caactcgttt gcagtaaaat gaaactcggc
    17161 gagcccgatg cctccggccg ccgctcccct gttgatactg gcgaaacatt tacattggat
    17221 gttgatatga taataaaagc cgctggtcaa actccctatg aaaaactgat taaaaaactc
    17281 aacattcaac attcaaaatt caaaataaca attgaaaatt gcgcaacaaa cataagaggt
    17341 gttttcgccg gtggtgatgc agtgaatggt ggtaaggaag tagtggatgc agtacaggca
    17401 ggaaaagacg gggcggctgc tattttgaag tttctgaaat tatgttgaat gttaaatgtt
    17461 gtatgttgaa tgcatttcat ttaaaattta gcattcaaca tttatcataa ctcttaaatc
    17521 gttttgaatg aaagaagaca acaaaatatt gaacctgact ttcgatttct cgattcagat
    17581 tattgggtta tataagttat tggttcagca gaatgaatat gtaatatcaa agcagctatt
    17641 aagaagtgcc acaagtattg gtgctaatgt ggaagaggct accgcagctc agacaaagaa
    17701 agatttcatt atgaaaatgt caatcgcttc aaaggaagcc agagaatcaa aatactggct
    17761 acggctttta gataaaagtc aaatggtaaa agccgattat tcagtttatc tcacctgtat
    17821 tgaacacatc ataaacatcc ttaccaaaat cgtcaaaaca tcccaggaat ctttgaactc
    17881 aaaataattc aacatttaac attcaaaact caacataaat ttctccccat tcaacattta
    17941 acattcaaaa ttcaacataa atggcctcta ttgaatcaac tnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nccattcaac atttaacatt caaaattcaa cataaatggc
    18121 ctctattgaa tcaactttcc tcggcattaa atcccccaat ccttattggt tagcttctgc
    18181 tcctcccacc gataaaaaat acaatgtact ccgtgccttc gaagccggct ggggtggtgt
    18241 ggtctggaaa acgctgggct cgcaggtaaa aaatgtttcc tccaggtatt cagccgttga
    18301 ttttggcggg cagcgggtca tgggctttaa taatattgaa ctcatcagcg accggccgct
    18361 ggacattaat ttaaaggaga tcgccgaatc tgtccgctta tttcccgacc gggccatgat
    18421 cgtttccctg atggccgaca acgataaaaa aagctggcat gaactcatca aaaaagtgga
    18481 agacacaggt gcccatggac tggagctcaa cttcggttgc ccgcatggca tgacggaaag
    18541 aggcatgggt gctgccgtgg gacaagatcc cgagatcgcc tgcatggtgg tggaatgggt
    18601 aatggaaacc gccaccctgc ccgtgatcac caagcttacc cccaatgtac actccgtagt
    18661 gcccaccgga cgttccgtgg taaaagccgg aacgaatgct ttatcactga tcaacacgat
    18721 acaatcggtg accggaattg atctggacac ccttgtgccc aatccttatg tggcgggcaa
    18781 gagtgtgttt gggggttatt gcggcccggc cgtaaaaccc attgcgttga aaatgctcac
    18841 caccattgcg caggatgagc tgacaaaaaa agtacccatc agcggtattg gtggtatcag
    18901 cacctggaaa gatgccgtgg agtttatgct cctcggtgca tctaacgtac aggtatgcac
    18961 ggcggccatg aaacatggat tccgcattat agaagatctc tgcgaagggc tgaataactg
    19021 gatggacgaa aaaggctttg ccaccctcaa tgattttatc ggaaaatcgg tacccacgat
    19081 tacgcattgg gaagacctgg acatcaacta tcatcatatc gccaaaataa accaggacaa
    19141 atgtattcac tgcggcctct gctatattgc ctgcgaggat acctcgcacc agtccattaa
    19201 tcttatgtat ggcaaacctt acaataccta cacgataaaa gaagaagaat gtgtgggctg
    19261 caatctctgc cagctcgttt gcccggtaga tgattgcatt acgatggaag aacaccgggt
    19321 ggccccggag cctatcaact ggaagcaatg gcaggaaagg ggattgccgt tgaatgatca
    19381 ttaagatgat tatatactaa gctaagtgtt gctatcgtcg ttcgttacgt cgcactcttc
    19441 attttttggt aattctattg agcatatgcg aggttttcct tcttattttg aagtatcttt
    19501 aattgtgata ctattagaaa taaaaaccat caacgtcgta taaatcagta aatcatggga
    19561 agaaaaaaga cacacgataa tttaccatcc tttgatgaat tgattattcc aacacttaaa
    19621 gctcttcttg aattaggcgg atccggtaca atcgaagaaa taagtgccaa agtttatgag
    19681 atagaaaaat tctcagagga aatattacaa atacctcatg gtgatgatgg agggagaagc
    19741 gagattgact acagacttgc ttggagtaga acctatttaa agaagtacgg tttgttagaa
    19801 aattcttcaa gaggaatttg ggcattagcc aaaactgatt ttgacataac ggtgcttgac
    19861 tataatgaaa ttgtgagaaa agttaaagaa gacgaaaaac aaaaacaaac aatagaaaaa
    19921 aatcacgcca ctaaaaagga aattgaaaaa gaaattattg aagaagttga aacaacggaa
    19981 gagtggaagg aaaaattatt aaatatacta tatgaaatta ctcctgctgc ctttgaaagg
    20041 cttgcacaaa gaatattaag ggaaagtgga ttttttcaag ttgaagttac tggaaaatct
    20101 ggcgatggag gcattgacgg aaaaggcatt gttagagtaa gtgggctgct aagctttcat
    20161 gtaatttttc aatgtaagcg atataagagt tctgtaaccc caagcgaaat aagagatttc
    20221 agaggggcaa tgcaaggaag agcagataaa ggacttttta ttacaacagg aaaatttact
    20281 agagaagctc ttaaggaagc tacccgagac ggagcaccac ctattgattt aatagatggt
    20341 gaattattat gtgacaaact caaagaattg aagctaggtc ttgataccaa attgacagaa
    20401 actgttgaaa taaaaagtga ttggtttaag aacatatagt tttcactaca ccaaattcct
    20461 ccgcatagca gcgtctgcct gtcccccgca ggcgggggcc ggatcgccaa acaggttgca
    20521 ttaccaaacc ggggtggaag aaacctggca ccggcgttag ctccacccct gttcggagac
    20581 tcattacaat cctaacactt cccccgcctg cgggggatag cggagagcct gcacttcgta
    20641 cacattactc ctttgaagca ttcttcttct ctatccactc ttccaggtag ctatgcaccc
    20701 attgtatatt cgtcagcact tcatgattag taaaccgcag cacagtaaac cccgcttctt
    20761 ccaacacctt ctgtctctgt tcatcattct tccttacatc ctccagttca tgaatacttc
    20821 catccacttc aataatcagc attaattctt tgcacataaa atcagcaata tagttcagta
    20881 ccggtcgctg ccgcctgaag ccataacctt tcatctttcc cgcccgcaaa acaaatttcc
    20941 aaaggcatgc ctctgctttg gtcatttcct ttcgcagacg gtttgcattt ggctgcaggt
    21001 tcttattata aaaatgattg ctgtatcctt cggtcatctg ttgaaattac aaaagaaaat
    21061 aaattacctt tctcgcaaaa tgaagaacta catgagaaaa ataatctcct tcatgcacct
    21121 atcgcttgac ggttttgtag cagggccgaa cggagaaatg gactggatca aagtggatga
    21181 tgaaatcttt gatcatgtcg gtaagcggat aagcgaaggc gacactgcct tatatggtcg
    21241 ggtaacttac cagcttatgg aaagttactg gcctaccgca gccaacaaac ccaatgccag
    21301 caaacatgac attgaacatt cagcatggta cagcaaagtg cacaaattgg ttttatcaaa
    21361 aacaatgaaa gaagcggatt tacctaacac aacaattatc agcgaaaacc tttcggacag
    21421 cataaataaa ataagacaac aggcaggccc tgacatcctg cttttcggca gccccactgc
    21481 cactcattca ttaatgaaac taaatttaat tgacggctac tggctatttg ttaaccctat
    21541 cattcttggc cggggcatcc ccttgtttgc agatagcaaa gaaaaaataa aactgaacct
    21601 ggtgtcaacc cggccattta cctgtggggt aactgaactg aattacacag tggacagaca
    21661 ataaaccgct ttctttaacc acggagacac aaaggcacag agtaacacag tgaatctcca
    21721 tgcatcaccg tgtcaccgtg cctccgtggt tcaaaaacaa tccgctattt agcaggcaag
    21781 caggtataaa aaagacagat atttgtcaaa acaaccgatc atgaaccgaa gcatttgcag
    21841 gcttgccgta tcagctctcc ttgtattgta cctgcccgca tcggcacaag atcaggcaaa
    21901 gaaaaaaaat atcatactca ttatttccga tgatcatcgt tttgatgcgc ttggcattgc
    21961 cggaaatact aagatctcca ctccccaact ggatgctctt gccgtcaccg gtttttattt
    22021 tccgcaggca acgatccata gcccgcaatg ttcgcccagc cgttctactt ttttatcagg
    22081 acaacctgcg catcgtcacg gggtcttctc caacgatgtg ttgaaagaag caaaagattc
    22141 gtcccgcttt tttcagcagc ctacccttcc ccaattgctg cagtcggccg gctatcatac
    22201 ttttctttcc ggcaagtggc atgtgcccgc cgatccctgg aaaataggtt ttgaagaaat
    22261 ccgctactgg atgcccggtg gcgccggtgc ctataaagac ccgcagctgg cttttggcaa
    22321 gagtaaggag aagaaaaaga cgattggctt caccaacgag atctttgcca aagagatcat
    22381 ttcattttta gaatcacccg cagcaaaaga aaaacccttt tttgtttggt tctccgccac
    22441 agcgccgcat gccccttaca aaccaaaccc gaagagaata caaaatatct atgagggcaa
    22501 aacagcaaaa caactattgc cccctgcttt tccagaacaa tatactcccg gtgattttct
    22561 gcattactac caggccatca gttttctcga cgagctggta ggccggatca tacgaactct
    22621 ggataaaaca gaactcagcc gggaaacgat catcgtcttc atcggtgata acgggctgat
    22681 gatgggcagc cgcggcatga aagggaaagt aaatccctat gaagaatcgg ttagagtgcc
    22741 gctgatcatc cggatgccgc aacaaaatga cacggtacat acgatcactt cccccgtttc
    22801 aagcctggat atttctccca cccttttagc tttggccggt gtgaaagtcc ctgcatcatg
    22861 gcccggaaga gatatctccg gcttgctgga aaacggaaag aagaaccaac tggattatgc
    22921 aatcactgag tgggcagaca acgaacatgg ttatgcagcc taccggctga tccgcacaaa
    22981 ggactataaa cttattgcct gggaaaatct tacaaaacca atggaactct accagctgtc
    23041 caacgatccg caggagacag caaatcttgc aggcaatgct gattataaaa aaatccagca
    23101 agaattgctg agccagttaa agcaatggat gagcaagacg aatgatcctg cattgaactg
    23161 gaaaaattta cctgggaaat aatccggtac tgcgggccaa cattaatgca atagcatccg
    23221 gaagtaaagc cacaaaggtt tcccgccgtc ttatttattt gcctgaaaac ttatcgcagc
    23281 attcttccat tgctcttcgt cccgtttttt cttataaaaa atattttgcg aaaccgaata
    23341 gttgcgcata tatttgcaac caaatggttt cataaaatca accagtatga gacgagacat
    23401 tttccaggcc attgccgacc cgaccaggcg ggccattatt accctgattg cattagaggc
    23461 catgacaccc aatgccattg ctgaacattt cgacagtacc cggcaggctg tttccaaaca
    23521 tctgcgcatt ctcactgaat gtgaactggt aaaacaggaa taccagggca gggaaattta
    23581 ttactccctt gaaattgaaa aaatgaaaga gattgataaa tggttagacc agttcagaaa
    23641 aatctgggaa accaaattca atcaactgga taaactatta gcaacactta aaaaacaaaa
    23701 aaaatgaaca ccaacctgtt attcgatttt gttgttgaca aaacaacaaa gacggtatgc
    23761 ataaccaggg aatttgatgc tgacctttca ctggtttggg atgcatttac gattgcggaa
    23821 ttgattgatc aatggattgc accaaaaccc tttacagcca aaactaaata ccaggacttc
    23881 aaagtcggtg gtaaacgatt ttatgcgatg ataagccccg atggacagga acgttgggtg
    23941 atccaggaat acacctccat taccccgaag accaatttta aaatgtataa tgcatttgca
    24001 ggtgaaaatg aaatccctga gccatcaggt tctgagtggg accacacttt cagcgaacaa
    24061 aacgagaaaa caaaagtgaa catttcgatc tacaatgaat ccctcgaaag gatggaaagg
    24121 atacttgatg gtttcacaca aggaatgaaa ttatcattga ctaacctgga aaacctgttg
    24181 gcagtattat ccggtaaata aactatcatg aacgtaaaag aacaaatcaa aaactatatt
    24241 gccgctcagc cggaaccaaa acgcagtgac atgcaggaac tgcataagct aatagtgaac
    24301 cggttgccaa ccggtaaatt atggttcctc gacggaaaag acagcaaagg gaaaattgta
    24361 tccaacccta atatcgggta tggctcttac accatccact atactgaggg aaccaccaaa
    24421 gagttttacc aggtgggttt gagtgccaac agttccggta tctccgtcta tatcatgggt
    24481 ataaaagaca agacatacct gtcccgcacc tatggtaata aattaggcaa ggcaactgtg
    24541 acgggttatt gcattaagtt caaaacggtg aaagatatcg acattgctat acttgaagat
    24601 gccattcgat atgggtttga gggcaacatg atcagttaag caaatgtgga tttatcttac
    24661 atttggaata tgcaaattcc aaaaaacctt atttaaattt aatcttatga aacccgcaaa
    24721 atgcatcttc ttcattgcag gtttgttttt attcccttat aaaaaactat atagtcaaag
    24781 cctgtccaat tttgtttcag taaatcccac tgcgcaaaca agcaatttta tattcccctc
    24841 tgctacacac cggtttcaaa aaataatgga gcatggtgat gtgcttccag tcggcaccat
    24901 gatggataat tttgatttca caggttatat gcccattgcc ggctcctcaa tcaatgggta
    24961 cctgagtatt aatcatgaac aaacaccggg tggtgttacc gccatgaatg tttcttttaa
    25021 ttccatcacc aaactctgga ataaaaacgg ggcaacagca atagattttt cttccgtagc
    25081 aggtacggcc cggaattgtt ccggtggttt aactccatgg gggaatatca taacctgtga
    25141 agaagtgatt tcaatagctg atgtaaatgc agacggttat aacgacctgg gctggcatgt
    25201 agaaattaac ccgtccacta aaacagttgt aagaaaactt tgggccatgg gatgcggagc
    25261 taaagaaaat attgtcattc attcaaacag gagaacggct tattttggca atgatgcaaa
    25321 tcccggatac ctgtataagt ttgttgccac tactatggat gacctctctg ccggatcatt
    25381 gtacgtatat acaggaccta aaaccggaaa tggcaattgg gtgctgctaa ataatacaac
    25441 ccaaccagac agaaataaca ccatggtatt aggtaatgct gcaggggcta cggtttttaa
    25501 cggcatagaa gatgtggaga ttggccccga cggtaaaata tattttgccg taaaaggtga
    25561 aaacagggta taccggttcc aggatgctga tcccgtttcc gggaccacca cttctgccat
    25621 ggaaacttat gtaggcaatg catcgtataa tattacccat gcaggaggaa ccgtatccac
    25681 tccctgggga accggaaatg ataacttagc atttgatgaa ctgggtaatt tatgggtgct
    25741 ccaggatggg ggcaataact atatctgggt agtgatgagt aatcataccc aggccgttcc
    25801 caatgtaaaa ttgtttggca ttgccccggc aggagcagaa ccaacgggaa ttacatttac
    25861 cccggattat aaattcctgt ttatttcatt tcagcatccg actgctacca ataattcaga
    25921 tctgcaaatg gatgctgccg gaaacagcat tggttttgaa aaagatattg ctatcgcaat
    25981 aggagtaaac agtgagctgg gatgtattac gggtgatggt tgctttgttg caacgaatac
    26041 gaataatgtt ggtgttggta cagataaccc aaaggcaaaa ttgcaggtaa gaaatggtga
    26101 tgtatcatta gaaactattg gtacaggtat tattctgaaa tctcctaatg gcaattgctg
    26161 gaaaattact attagcaaca ccggagtgat tacttctgtg gctgttcctt gcctggaata
    26221 agacattgtt gttgacatca tcagcaattc cttccaccca gggcagggtc ttgtcacaca
    26281 gcctgtacaa cataattgaa tgagaagggc gggatctttc ttccatagct ctccgcaaca
    26341 actgaataaa tcattaactt cctctacagg aaattagctt tacttaatag taataattaa
    26401 aatggataat cacagagtat ttaaaatgtc atttgccggt gtctatccgc attatattca
    26461 aaaagcggag aaaaagggac acaccaaaga agagctggat actatcattt gctggctcac
    26521 cggctatacc caaaaacaat tgcagcaaca gatcgacaat aaaattgatt ttgaaacctt
    26581 tttcgcacag gcaccacaac taaatcccaa tgtgtcaaag ataaccggtg ttatttgcgg
    26641 ctaccgggta gaagagattg aagataaact catgcaaaag atccgttacc tggataaact
    26701 ggtggatgaa ctggcaaaag gaaaggccat ggagaagata ttaagaaagt aagagcacca
    26761 ggttccgaca cagcgattta ctgctgaatg accactaagt acaattactt tctctttctg
    26821 gcttcaaatt tgcaattttg ggagtcaaaa ccatcacaag cagcctgtta tgaacaagaa
    26881 actgaagaaa attgtaaaga tcacaggttt caccctactg gtactgatcg ccgcagcctt
    26941 tttgattccc ctattattta aaaaacaaat aacggagttg gtcaaaaaag agatcaataa
    27001 taaccttatc gccaaagttg atttcaaaga tgtaagcctt tctctcttcc gtcatttccc
    27061 caaaatttca attttgctgg aagacctgtc tgtagtcggc acaaacgaat ttgacagcga
    27121 cactctcctg tctgctaaaa cactggatgc ctctgtcaat cttatcagtg ccatcagggg
    27181 aaaaaatata aaagtatatg gtgtgtatgt gcaatcaccc cgcatacatg cattgatcaa
    27241 taaagaggga aaagccaact gggatatcgc caaagaaagc actgactcat caggcaaagc
    27301 agacaattct ccttccgaat ttaaaatgaa cctgcagcag tacgagatca gtgatggata
    27361 tgtttattat aaagatgaaa gtattgattt gagtgccgaa atatccggtc ttgagcataa
    27421 aggtagcggt gatttcactg aagatgtgtt cacactttcc actaaaacca aagcaacatc
    27481 aactgatttt acttatgctg cagttcctta cctggtaaat gcccaaacag acattgatgg
    27541 agatatacag attgacaata ccaacagtaa atacagtttc aaaacagagg cgatcacggt
    27601 caataaactg aaattaaatg ctgatggttt cttccggttc gtgaatgata gtacgtatga
    27661 catggatatt aagttcaaaa ctccgtccaa cgattttaaa gatatcctct cgctgatacc
    27721 cggtatttac aaacaggatt ttgcaactat taaaaccagc ggcaatgctg cctttaatgg
    27781 ttttgtaaaa ggaatttaca gtccgcaaca gttaccggct tacgatgtga acctggaagt
    27841 gaaagatggt ttcttccagt atcctgatct gcccaaaccg gtaaagaata tccagctggc
    27901 catgcatgtc agcaatcccg atggtaaaaa agataatacg gtagtcgata tttccaaagg
    27961 gcatgtggag atggataatg aaccttttga tttcaaactt ttgttcagga accccgaaac
    28021 ggcccaatat attgatgccg tagcgaaagg caaactggat cttgctaacc tttcgaaatt
    28081 tataaaatta gaagccggaa ccaaactggc aggactggtt tgggccgacc tttttgctaa
    28141 aggcaatatg tctgcgttgg aaaaccagca gggcaatttc acagcaggtg gtttcctgga
    28201 tattaaaaac ctgttctatt cttctgctgc atttccgcaa cccctggaaa atggaaatat
    28261 gaaagtgcag gtagaaaata gcggtggcat tgccgacaat acatccataa atataactac
    28321 cgggcatatg gaagtgggta aagaccctgt tgattttaat ttgcaactac gccacccgat
    28381 gtcttttgtt gattttactg gtgcagccaa aggaagattt accttaaatc atatcaaaca
    28441 gtttgtaacg cttgatccgg gaaccagcat cagtggaatc ctgaatgctg atcttggttt
    28501 cagtggtaat aaaacagcga tagataaaaa agagtatgat aagatcatca tcaacggtac
    28561 agccggattg aataacctca actatgtatc gaaggattat ccaacaggat tgagtataac
    28621 gaattcagca ctcagcttcc aacaaaaaaa tgtaacactg aacaatcttg ccggtaatta
    28681 cctgaataca aatttctcgg ccaacggtac attcaataac ctggttggct atgctatgca
    28741 ggaccagccg ctggcgggaa cgctgaatgt aaccgccgat aaaatgaacc tgaatgactg
    28801 gatgggaaca gataccacga cgacaacaac cagtgccgct gcatcagccg atccgtttgc
    28861 cgttccggcc aatatgaacc ttaccatcaa tgcaaaagcc ggaaaagtaa aatatgataa
    28921 ggttgattat actaatatca ccggaacatt attgctgaaa gatgagacgg tgaaactcca
    28981 aaatgtaaaa acagaagctc ttgacggcac catagtattt gacggttctt attccactaa
    29041 aaacaataag aaggatcctg ccatttcaat gacctacgag gttaaaaata ttgatgttca
    29101 aaaggcattc ttctccttca atacgattca gaagctgatg cctgtaggac aattcctggc
    29161 aggcaagctt aattcgcagc tgaccatgaa cggaaacctg gatggcaata tgatgcctga
    29221 cctcagcaca ttgaatggaa agggaaatct tttactgata gaaggaatat tgaaaaaatt
    29281 tgccccgctg gaaaaaattg ccagcacact tcagatcgat gaattgaaat cgatctcgat
    29341 aaaagatgta aaaaattata ttgagtttgc caaaggcaaa gtgctggtga agcccttcag
    29401 tgtgaaagta aaagatattg aaatgctgat cggtggtatg catgggtttg accagtctat
    29461 cgactacgtg gtgcagatga aagtgccgag gaaatacctg ggcaatgaag gaaataatat
    29521 actcaataac ctggtgacac aggctgtaaa taaaggcatc ccggtaaaac tgggagaaac
    29581 ggttgatctc agtttcaaaa tgggtggcac tcttgctaat ccaattttaa gaaccgaact
    29641 gaaagaggtg gcaggagatg ctgtcaaaga aatgcagcag ctggcggttg attttgcaaa
    29701 agcaaaattg gatacagcga aacaaaccat aaaggacagt cttactggtg ttaagaacca
    29761 ggtgttaaat gatttgaaaa aagaagcact tgacaggatg tcaggcaata aggattcagt
    29821 aaaaaccaac ccggctgata gttctaaaaa gaaatcagag cagaccatca ggaatacgat
    29881 caatacgctt ttcaataaaa agaagaaacc agccagtgat acggcagtga agaactaaac
    29941 aaactttttt tattcaagga gttttcaacc ggcaagctgg gttcaatttt ccttctctcc
    30001 tgtttcttta caccaggagc aggaaataaa gtaattttat ttcaaccaat caaccgaatt
    30061 tctattaaag tatgagtact aaaaagaaat tatctgcagc tcaatcccag gaactgctca
    30121 gtaccttaaa aacccgtttt gagaaataca tagatcgcca taaaggcata gaatgggcca
    30181 aagtacaatc aaagttggag gccaacccgg ataagctgtg gtcactgcat gaaatggaaa
    30241 ctaccggtgg ggaaccggat gtggtaggtt atgataaaaa gacgggtgaa tatttttttt
    30301 atgattgttc ggcagaaagt cctgccgacc ggagaagtct ttgttacgat gccgcagcgc
    30361 tggcatcccg aaaagaaaac aaacccaaac acagcgccat gggcatggca gtggagatgg
    30421 gcattgagct attatccgaa gaggaatacc gggccctgca acagttcggc gaattcgatc
    30481 taaaaacctc cagctggata aaaacaccgg acaacatacg caaactcggc ggcgcccttt
    30541 tttgtgaccg tcgttatgcc acagtcttca cctatcacaa cggtgcagag tcatattatg
    30601 ctgcaagggg attccgtggt tcgttaaggg tttaaat
//
LOCUS       DAORTI010000029        29797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1099_length_29797_cov_7.603490, whole genome shotgun sequence.
ACCESSION   DAORTI010000029 DAORTI010000000
VERSION     DAORTI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_29797_cov_7.603490"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..620
                     /locus_tag="PLO70_05835"
     CDS             231..620
                     /locus_tag="PLO70_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ74014.1"
                     /translation="MKQKSILLAFCLLGFVAANATNGPGDGKKDELNGIVVHAETKKP
                     LKDVSVTAYLSSRKEKIVLSDFDGGFSFDELKPGTYKFVFEKSGFKRIIKDKVVIRTD
                     EAFQMNIEMIQHSDFEIMPSPFNVGGI"
     gene            complement(701..1537)
                     /locus_tag="PLO70_05840"
     CDS             complement(701..1537)
                     /locus_tag="PLO70_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74015.1"
                     /translation="MKPTTFTTGRVALVLLTIGLTIGLVSWDYKQSPGQYQQSATDTV
                     PKKSKDREKKVRDLDDVLTELDNADLNVDMEKIQKEIAEAMKNIDGDKIRLDIEKAMK
                     DVDFDKIKKEVETSLAKVDWNNIQTEITNAMKDINMAKIQQEVQASLAKVDWDKMKTE
                     LDKVKDIDMKKLEAEMNKVKEEMKDLGPKMEKELQKAKVEIEKAKAEIKEYKEFVNGL
                     ESDGLINKKEGYTLKHKDGELFINGKKATEQTYNKYRNFLDKHKKFSIEKDDDDFDID
                     MD"
     gene            complement(1703..2980)
                     /locus_tag="PLO70_05845"
     CDS             complement(1703..2980)
                     /locus_tag="PLO70_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ74016.1"
                     /translation="MNKIWIIIKREYLTRVRKKTFIISTLLFPLLYLGLIFGMGFIAE
                     KSKQNLHVAVIDSSGYFSTDAINKQNLADNSSVLTLVKSNTDKIIADHNAEGYDGYII
                     IPAINWKEGSDSLFFKANKSYGSGSTAAVEAKLNRIWNEIKNDSLQIDTEKQTILVKS
                     KLRVSARNIKDENANVKTAEGIGYVAGFLIYFILLIYGSQVMMGVMEEKTNRIAEVIV
                     SSVKPFQMMLGKILGIGLVALTQFFLWIAFVLIVYNVGKASGAGGGMATEMVGSMQKM
                     FASVNVPLILFCFGFYFLAGFFFYASLYGAIGSAVNEDMREAQSLSFPITMLVIISIA
                     LMTAAISDPTGPIAVWGSIIPFSSPIVMMARIPYGVPGTVPVWQLALSMGLLILGFIF
                     TVWFAGKIYRTGILMYGKKPSWKEMIRWAFRKN"
     gene            complement(3006..3944)
                     /locus_tag="PLO70_05850"
     CDS             complement(3006..3944)
                     /locus_tag="PLO70_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ74017.1"
                     /translation="MPSILELQNLKKYFATQKAVDDISLNVERGQIFGLLGPNGAGKT
                     TLIRMITGIFYPDEGQIIFDGKKFDPVNDVGQIGYMPEERGLYKKMKIGEQAMYLAQL
                     KGLSHKDAMAKIKKWFIKFEMESWWNKKVEDLSKGMGQKLQFVTTVLHEPKLIILDEP
                     FSGLDPVNSNLIKDEIYNLAKSGSTIIFSTHRMEQVEEICNHIVLVNKGKKILDGTVK
                     QVKQEFKENLFSIGTEQMLSSQNGNAPFELVGSADHSHVVRIKEGQTPNNVLQYLLQQ
                     GIAIHSFNEILPSLNDIFIKLVEGTPTARQFQKVEA"
     gene            complement(4046..5611)
                     /locus_tag="PLO70_05855"
     CDS             complement(4046..5611)
                     /locus_tag="PLO70_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ74018.1"
                     /translation="MQKTISLQLLPSEAANDTAIKKYIAKAEAIKVPAISGFTILKQS
                     IDARGRQPWINLSLKAYINEPFQQREMLSFDFKDVKNAERTIIIVGAGPAGLFAALQL
                     IESGIKPILLERGKDVRARRRDLAILNKEGVINPESNYCFGEGGAGTYSDGKLYTRSS
                     KRGDINRILNLFVHFGAEENILYESHPHIGTNKLPHIITAMREKIVACGGDFLFEKKV
                     VDILIKEDKITGVKTADGNIFEADAFILATGHSARDIFQLLYSKKLLIESKPFALGVR
                     VEHPQALIDSAQYHCQVRGDFLPPASYSWVQQVDGKGVFSFCMCPGGIIAPAATSPGE
                     LVVNGWSPSKRNNPYANSGIVVTVEEKDIQVFKQHGPLAAMFFQQSVEQRAFEAGGGK
                     FVAPAQRLVDFTENRLSSSLPGCSYLPGIHSSSLIGVLPPVIHKSLQQAFKDYGRKMK
                     GYYTNEAVVVATESRTSSPVRIPRDAVSLQHPQLKNLYPCGEGAGYAGGIVSAAMDGE
                     KVAKQIITILKAT"
     gene            5733..6485
                     /locus_tag="PLO70_05860"
     CDS             5733..6485
                     /locus_tag="PLO70_05860"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQZ74019.1"
                     /translation="MHSRLFFPTALLLIFIAGNSCNTTYQGQALQYKSYRIANTQQQD
                     SALLTFLKPYSDSVNNSMNGVVGIAEMSLDKKQPESTLGNFMVDAFLIMSREKYNTEV
                     DVAFVNFGGIRLTQLPAGNVTRGKIFELMPFDNLLILQKVKGSVLQQLLDLTADRGGW
                     PMAGLTMQIKDKKAVNVMIGGKPIDPDRLYTIANSDFIANGGDNADMLRPIPQITNGY
                     LMRDAIFDYISKLKSQGKNITANIENRVTNAQ"
     gene            6475..7413
                     /locus_tag="PLO70_05865"
     CDS             6475..7413
                     /locus_tag="PLO70_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HQZ74020.1"
                     /translation="MLSRKKFIRQAAMSAGAIMAGPSLLEAATYFSPLKLTILHTNDT
                     HSRLEPFPMDGGRNQGLGGVAGRARLINQIRKEEEHVLLLDAGDIFQGTPYFNIYKGE
                     PEIRAMSTLGYDACTIGNHDFDAGMENLATQLTRHANFPMLVCNYDFSGTPMEYKTQP
                     YKIFKKGNLKIGVFGVGIEGKGLIPDNLFGATKYLDPVQKANEVAYELKKFKYCDMII
                     CLSHLGNQYKGNDMVSDEVLAKQSENIDLIIGGHTHTFLNEPLVYKNKMGSDVVVNQV
                     GFAGIILGRLDFEFSKFSGKKLAKSHSISVSQKSGG"
     gene            7548..8981
                     /gene="dnaA"
                     /locus_tag="PLO70_05870"
     CDS             7548..8981
                     /gene="dnaA"
                     /locus_tag="PLO70_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQZ74021.1"
                     /translation="MEKNADKIWTNCLKIIKDIVEWQHYKTWFEPIKPVSLKNNVLVI
                     QVPSQFFFEYLEEHYVNLLAKTLKRELGKDAHLEYRIMVDSGNSKNKPVTMDVSGQGF
                     KTFSNNEMDFPLVINNPVKNPFVIPGLKKMQIDPQLNHIYTFESFIEGDCNRVARRAG
                     KTVAEKPGTNSFNPLVIYGGVGLGKTHLAQAIGNEVKRLHPNKVVLYVSSEKFINQFM
                     DHSRNNAINDFIHFYQLIDVLIIDDVQFFAKAEKSQDAFFAIFNHLHQSHKQLVLTSD
                     KSPKDLEGVQERLLSRFRWGLSADLQVPEYDTRIEILEKKMKNDGLEMPKEVVKYIAY
                     NISNNVRELEGALISLLAQSSLNRREIDLDLAKKVLRNFVKSSSKEITIDTIQKMVCE
                     YFDVPYDKLLQKTRKREIVQARQITMYLAKAFTKNSLKTIGEHFGGRDHTTVIHSCQT
                     VKDLMDTDGVFRENVLELQQKVQLAAM"
     gene            9124..9214
                     /locus_tag="PLO70_05875"
     tRNA            9124..9214
                     /locus_tag="PLO70_05875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9158..9160,aa:Ser,seq:gct)
     gene            9780..18782
                     /locus_tag="PLO70_05880"
     CDS             9780..18782
                     /locus_tag="PLO70_05880"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74022.1"
                     /translation="MRKFKVSTFTRFLLVTAFLITMSYSESKAQLDISIGTGTTGNTT
                     TTYPCPLQDFYEGSRMQFLYRASELNAAGMAPGLISSIKYNVVSLGTAGLVEGMVIRI
                     GHTTVTTLSTSAWDPFSGVPVSTVSTDYQPVNGINTFNFPVPFSWNGSDNILIEICNG
                     EPGNATGTWFTSNPVIPWTTDLTFNGSHNYRADNLGNLCSSATTTNTGTQTTRPNITF
                     SWTPAIACSGTPTGGTAAANPSTVCSGQNFDLSLSGFTIASGITYQWQSSADNSVWSD
                     ITGATSSFLTTSQTVTTYYRCIITCSNSGLSANSSSVLVTSPALVSGTFTINSALPTS
                     GTNFQSFNAAYNYISCGISGPVVFNVDPASGPYTEQLIINPVPGASSTNTITFNGNGR
                     VLQFTSTNTNERGVIKLNGADHFVFDSLTINATATTTTEYGFAVQLLNNADSNIIRKC
                     IINVTDAQTSTNHVGISISASATSATGTGNAECDDNLFELNTITGGYYGITNVGSTTV
                     ANQRNKFIRNIIKDYYLYGIYTSGTFTSLIEGNDFSRPARANIGTGTSYAIYFTSLST
                     SARVNGNYIHGLLDVNTASTNDVYGIYFTGVDAIAGLENIVSNNAIYDIKSNGTVYGI
                     YNASSDNIFYYHNTISLDDVASTTTEVTRGFYQTTAAAGIEFKNNLITIRRGGTGQMH
                     GLFFNTTTSTIVSNNNNVFFTPAANVFYGSWGTTGQATLANWQTASSQDANSAYIDPA
                     YTNLGLGNLKPTSAGMNDLGSAVGITADILGVSRNATTPDIGAWEFDVGGCTAPPNAG
                     DATASISVPICPNELVALGLVNNTFGTGQTYQWQTATTLAGPWTNIGPSQNNPAYSVN
                     PTVTLYYRCAVTCSGSTTFSTPVQVVVNALFPAGTYTINSALPTGGTNFQSFNDAVFA
                     LRCGIGGPIVFNVVSGSGPYNEQVIIGQIQGSSAINTVTFNGNGETITNLSTNTNERA
                     VIKLNGTDHFVFDSLTVVPQGAATTEYGFGFHLLNSADSNIIRNCDINMSTTTTSTNF
                     AGIVSSVGTTATTTGTNLSDYNLFENNEITGGYYGITSVGSTTSAVGNNAMVRNTIKD
                     FYAYGIYLSGNFNALVEKNNIFRPARTASTTTYGIYVTSLNTGVKLSKNRIHNLFDGA
                     PTATSTSYGIYFTGVDAIAGLENIVSNNAIYNMNGNGAQYGIYNTSSDNVFYYYNTVN
                     LDDATATTTSDAYCFYQTTEAAGIDLKNNLFSMTRGGNGDKFCMYFATAATTFTSDYN
                     GVFRNAVNSHVGYNGSNQTTLANWQAATTQDGNSVSANPYYTDAAAGNLEPRTPALDN
                     EGTPVTGITSDIRDAVRSATTPDIGAWEFAVPVCTAPPTAGSATAVPNTGICLGTPID
                     LDLNGNSIGSGQSYQWQFATAAAGPYTNLGNPKLFPDTTILASGTFWYRCVVTCSSQS
                     ANSTPVQVNMNPPFPAGVYTINNTIPTDYPGGSNFNSFVEAVSVMECGISGPVTFNVA
                     AGTYSEQVRMHRITGASNTSRVTFQSANGNPASVILTYDATNAAANYVLQLDSASYIT
                     YRNMTITAINATNGRAVDIANLASNDSLVNNIINAPTATGTGTTVVGIHAANLAGGDH
                     TIKGNTVNGGVTGIYFGSTTTSPSAYNFIDSNTVTGSYYYGIYNSNTRFTSVSKNTVT
                     VAAPRNTTTYGIYLTSADSAYQVNNNKVDINGTTSTTYGIYLTGCGATGHTGSVSGNK
                     ITGLTGITGTLYGLYQTSSIGNYTKNNVININTSATTAYGLYSTGGNGIKYYNNSVQN
                     ASPAAGTTNIAAYISHTTSSAGVGDIRNNIFSHTANGIAANYLNVANIYTDFNMFYTA
                     GATLIRQNTVNYATLQDWKNGQNWDYNSIVYTPAFAAGNELQPDVTNPDVWAMHGRGV
                     QITDNATDINGNIRPTTLTAGVPDMGAYEFLPTVQPPVLPATPAAPAAGTTQVFMFGT
                     DTVQKITWAPASTVPTTVDVRRYSGIIPPGLAPGQQSMYYYTDVDITGSAPTNFTNQQ
                     FYIDPWMRDIPSEPTVKLGRTNTAGTWLVASGSVVDDLGNVITESNLSFLDKFTGMTD
                     GVAPPPAVPPVFTLDTSNRGKRFWVGYGHHYGFSSNSQDMALYLSAEDSANVTVKVNG
                     TNWVRTYAIPANTVRVSDFMPTSGLVDARLTDEGLFNKGISIESDVPIVAYAHISDGA
                     NSGAGMLLPTGVYGYEYRSLNSRQYYPTGGAGSYSWMFAIADHDSTLVEITPSVTTKG
                     GRPAGVPFQVYLKRGEVYNVMGTQATGGQGTDLSGSTIKSIPNASGKCYPIAVFSGSS
                     RTAICNTTNGDNMIQQVFPASAWGTKYLTFATANSASNTNYNSNGWRVMVKDPATVVR
                     RNGVILDPATLVVPGNYYEFFVGNNTGANTASFIEADQPVMVAQYMLSTSGTGCSGVP
                     APTGNGDPEMIYISPIEQGIKKARFYNTNNNSITSNYVNVILPTAALPSLQIDGSTTF
                     TDVFAHPNLAGYTCVRQNFAGAAAQHYITCDSAFTAITYGLGSVESYGYNAGTLVKNL
                     SAVPSFNNVFNTSGTNTYTCKGTPFRLSLQISVKPTQLVWQLSQVNNVTPNVNVTQVN
                     PVPTDSVTVNGKKFYIYTLAQQYTFSQVGTYNVPITVFHPSIEGCGGSVEIALPVTVI
                     AAPVSNFTTNFSGCVGDAVQFNATTTTSNGVPTNQWTWAFGDGNNGAGQNPVHTYATA
                     GTYNVSLRGIADDGCIGDTTKPVVVNPKPTGAITQDTVYVCNNASATFTVQNPVTGDT
                     YNWYSAATGGSLLGTGSTFVLNNVTGFVNVYLEVIRLGCASATRDRATAAILPTLPAP
                     VVTVDSVSTNTIVFRWTAVTGAASYQVSTNAGGTWSTPSSGPQGLTHTITGLPLGTSV
                     TLLVRALGGCLPAVSQPATGQTVTDQVYIPNSFTPNNDGLNDVLRVYSNVIRSMKFVV
                     FNQWGEKIFESNSQSVAWDGTHKGKPQPSGVYIYVCDIVLNTGERIQRKGSINLVR"
     gene            18809..23968
                     /locus_tag="PLO70_05885"
     CDS             18809..23968
                     /locus_tag="PLO70_05885"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ74023.1"
                     /translation="MKRKTQILFFTASLLLTGWLEGYCQNITNKGRDFWVGYGHHQFM
                     EPGQSNGQDMILYFSAEQAANVVVTVQGRTAVQTLNYAVPANTVISSAVMPKAGPNDC
                     RLYDVPPTFGGNGGEGLFGMSIHIVSDVPIVAYAHIFGSVSSGATMLMPVESWGYSYM
                     SINSQQIDAGGPGFSWVYAIAQHDNTVIQVTPSVTTRLGKPAGVPFNITLQRGQIYQF
                     IGQSDAGGNGNQFTGSTVKSIANAAGDCYPIAVFSGSSRTKGEQVPCGSSSGRDNDMQ
                     QCFPTQAWGKRYLTAPFSTSSGPSTAVILNASTFQTSVYKVAVKDPTTVVRRNGIILT
                     GLINNSYYQFSSNTADYIESDKPIMVAQFMSGAAGCNSGTWGDPEMVFLSPVEQAIKR
                     VGFYRNTRQSIYANYVTVIIPTAGVSSLRIDGSATFNHTYTHPNLPGYSIVVKGWQAA
                     QAQCQMYSDSAFTAITYGLGSAESYAYNAGTYLNNLNAISHTQNTPDPTVPQHPYTCV
                     GTPIKLSVLMAYQPTRLDWFLSQLGANIIPNTDIIDNAPVSSGTVVVNGITYYKYTLP
                     GTYIFNAADTFDLPVNGYHPSIENCYNRENLTISFIVKPKPLADFTFSHTGCTLDPVN
                     FSGVASTSNGYTIRDYNWTFPGPATATGQNVIRILPPGIHDINLSVVSNEGCASDTMK
                     RITIYDKPPGDFMVTPANLCEGSQFTITDTSSAAIAVNEWYWDFGNGVTQVVTNGPSV
                     TYTYPAFGTYTIKHVARSSASCVSDTVSRVVTVYAKPAVSFTNDAAGCLSPAGLVQFT
                     GTATTPDGQTISTYAWNFGDPNTTPGNPNTSVLQNPTHNYQQGTYTINFSATTANGCT
                     ENTTQTFTFNLKPALTYPLLNAVCENAPAYSIANATVTNGVTGTGIYEGPGTTAAGLF
                     TPAAAGYGTHTIWYKLTSGAGCIDSVSQTILVHARPRTNFTIPASGCLPANGQVQFTN
                     SSTIPDAQTMTWLWNFGDPNATPGNPNTSTAQNPAHNYLEGTYTINLLATSANGCVKD
                     TNITTSFAVTPLLSYAALPAVCENAAAVSVATANVTNGVAGTGVYSGPGTDAAGMFNP
                     AIAGYGIHTITYTFTSTGGCVQTTTQNIQVHARPRTSFTLPVAGCLPTTGQVQFTNTS
                     TIPDAQTMTWLWNFNDPNAGGANPNTSTAQNPTHNYLEGTYTINLQATSSNGCVKDTN
                     ITTTFAVTPALSFPALTNVCQNPGAAPSSIASASATNGVTGTGVYIGPGVNAAGLFDP
                     NVAGPGVHTITYTFTTAGGCTGTVNNTIEVYPRPTASFTASGNNGACLGQAVTFTNTS
                     AVPSGTITTWNWNLGDGNTPSYTNGNPFTVNYTSANTYNVQLVTVSDRGCFSEPVSQT
                     IRISPLPVADFTVPTGICMPGGTAAFTNLTAVADNSALTHVWNFGDGNTSTLTNPSHV
                     YAASGNYNVTLVSTSAFGCVQQVTKPMNSFFDKPLASFSVSPQELCQGADNVFTDGSS
                     APNSTIQSWAWNFADGGTSSASNPTKRFNTPGVYNVRLTVTNAVGCVSEPFVLPVTVH
                     LQPVIDAGQSYVLPQGTVLQLNATANSSNLVFNWSPGFGLSSTSILNPTLTALQDQVY
                     TLTATGDFGCTATDQMTVKILKQVKVPNVFSPNGDNIHDQWMIPNLGDYPGCTVEVFN
                     RYGQQVYFSPGYGNPWNGTYKGKELPAGTYYYIIQLQNGFKPLSGSVTIIR"
     gene            24023..25030
                     /locus_tag="PLO70_05890"
     CDS             24023..25030
                     /locus_tag="PLO70_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQZ74024.1"
                     /translation="MRKSLRISLLMLSIGLAVSKVNAQVDPHFSQYYVYPSWLNPALT
                     GAFDGQYRVSGVYRNQWGNISSPFTTYGASAEFTTEKKINLGVSVLKQTAGDGGYGYT
                     TAYGNIAYTGVRFGKNEEHRVVFGMQAGIIQRRFDRNKMTFGDQWNPVFGYNPGNPSS
                     DVLNRTSATAFDMGAGVLYFDAQPGKKANLYAGFSASHLTKPEDKFSASGDEKFPMRY
                     TAHAGIRFTLSDRFSITPNFLYLSQGTATEKMAGAYGQYKATSDVNLLIGANYRFEDA
                     ISPFFGFTYKNMMLGASYDINSSDLGKLVNGSNSFEISLSIIGKKSTKTPEVEFVCPR
                     L"
     gene            25069..27066
                     /locus_tag="PLO70_05895"
     CDS             25069..27066
                     /locus_tag="PLO70_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ74025.1"
                     /translation="MKKIFVLTAVLFSFASLHAQFVYDYLKAADDYFQKGDYASAAEY
                     YEKYMGGDKTGKDKEFKPYTPQNSSKKTAAAAVNKEQAMYNLAECYRLLNYPSKAEPQ
                     YKEVMVANKKQFPLAQYHYASQLRALGKYAEAEQNFKTFLSGYPANDEYKKNAERELK
                     NLQFIQAELVKKDLKYYTINKAGGELNAKGASYAPAWLNSNTLLFTSTRPQDTTAKTM
                     VYTNRVYQAVYDAGTLASISPATIPQEKGMQQGVVTVTPDGNTMYLTKWGISGDKKSA
                     AIYSSSKTNDGWSEPVKLGESINANGANTQQPFVTADGKYLLFSSNRTGGQGGYDLWY
                     APLENGTAGTPVNMGAAINTSYDEQAPAYHEASASLIFSSNGRVGMGGYDFFRSKGNI
                     GNWTEPVNMGYPVNSVKDDIYMVSRGSAKNNVLENVMLSSDRDAVCCLELFALQKVRP
                     LRQLSGRVLSCDPSKPLSGATVTIVDPASNKTIYTKEVGADGTYSFTMEDHQPLKVQA
                     EAKGFISNAANVGTPADLEEESMRYPDLCLVPEAPKVDEKFVIENVYYDFNKADLKPE
                     SYPALDEVVRMLNTYPNMAIELGAHTDSKGSDTYNMKLSEARAQSVVKYLVEKGISAE
                     RLVAKGYGEAMPIAPNEIDGKDNPEGREKNRRTEFKVLKNE"
     gene            27134..28162
                     /locus_tag="PLO70_05900"
     CDS             27134..28162
                     /locus_tag="PLO70_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQZ74026.1"
                     /translation="MKKYIFLVIMIVLGGMATLPVLAQQKPHYTQYILNQYIVNPALT
                     GIENYTDLKVSHRHQWVGIQDAPITSYLSVHGPIGKSDYRKTATSFDLKGENPRGKRY
                     WEEYTAPEPHHGIGLQVINDITGPLSNFSLYGTYAYHLALSARTSLSAGFGVGFNRIS
                     LNADKLDFGDVIADPAVFTSGILNKTRLDMMAGLYLYSADYFIGVSAQQVVPQKIDFS
                     NGYIRPKEGRTVPHLFGTAGYRFSLGDNFNLIPSLMVKYVDPLPVQVEGNLKLQYLDR
                     LWIGGSYRHKDGYAGMMGLNVSNIFHVGYSYDYTTSRLNNFSKGTHEVLLGFVIGGYP
                     DNCPRNVW"
     gene            28299..29594
                     /locus_tag="PLO70_05905"
     CDS             28299..29594
                     /locus_tag="PLO70_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HQZ74027.1"
                     /translation="MKRYLILTTVLFIAGSISAQKFPKPGCSTVLAEAMFSEVDTTKP
                     RGVSDNYHTWENGAVLLVKFMPGGSKMLRDKVILSAKEWEKYANIKFQFVADTVRNTN
                     IRVKLGKGSGHNSAVGTESNFRSQSEQTMNFDTLFFADIDYYLAKLQRKGIKPPYNLN
                     QLAAEMKEEPNRWNEKELRRVVTHEFGHAIGLLHEQSFPGAVNWKRTDSVYNYYLQTQ
                     GWDRAKVDFNVFEVADQFYTNGTTYDPKSIMHYSIEPWQTTDGYSLKDNYELSQGDKS
                     LIAALYPKNQKVSSLVVPKVDITNFAKLNVVSNTTRNGLVIEPSFDLKTNPKLGEVYF
                     VARLATEDGYYLKTSSLYYNWGGTVAAYKKMNLLPNTKVSYNKTKKNFELFLPFDYVP
                     ETYGKKVIVVFAVYLDDVANKQMDKLMYFSTTTPLSLPR"
BASE COUNT         8709 a         6030 c         6412 g         8646 t
ORIGIN      
        1 agggcaaaga aggggatgtt tgaaggtgat ttacaggaag gagagttgga gattggccag
       61 gtaagtgcat tgctggatga gattttacca gctgatcaga tagtgaaaaa tgtgtggaag
      121 gattttatgg acggtttgtc aaatccggta aaaaaattat aattttaatc cacaccacca
      181 aaaaaagaaa agtgctgaag ggttatttta aaaccattca aaaaagtaca atgaaacaaa
      241 aatcaatcct gctggcattt tgccttctgg gttttgtggc tgccaatgct actaatggac
      301 ccggcgatgg aaaaaaagat gaactgaatg gtatcgtggt gcatgcagaa actaaaaaac
      361 ctttgaaaga tgtaagtgtt acagcttact tatcatccag gaaagaaaaa attgttttaa
      421 gtgattttga cgggggattt tcatttgatg aattgaaacc cggtacttat aagtttgtat
      481 ttgaaaaatc aggtttcaaa agaatcatta aagacaaagt agtgatcaga actgacgaag
      541 cattccagat gaatatagaa atgattcagc atagtgattt tgaaattatg ccctcaccgt
      601 ttaatgtggg tggaatataa ataaaacttc agtttaaata aagagtcccg ctattgcggg
      661 actcttgctt ttcatggctg gttttggtaa acaggatcca ttaatccata tctatatcaa
      721 agtcatcatc atctttttcg atactgaatt ttttatgctt atcgagaaag ttgcggtatt
      781 tattatacgt ctgttccgtt gccttttttc cattgataaa gagctcgccg tccttatgtt
      841 taagggtgta tccttctttt ttgttgatca gtccatcact ttccaggcca ttcacaaatt
      901 ctttatattc ttttatttca gcttttgcct tttctatttc cacttttgct ttttgtaact
      961 ctttctccat ttttggcccc aggtctttca tttcttcttt tactttattc atttcggctt
     1021 ccagtttctt catatcaata tctttcactt tgtccagttc agttttcatt ttatcccagt
     1081 ccactttggc gagggatgcc tgcacttcct gctggatctt tgccatatta atatctttca
     1141 tggcatttgt aatttcggtt tgaatgttat tccagtcaac tttggccaat gatgtttcga
     1201 cttctttctt tattttatcg aagtccacat ctttcatggc tttttctata tccagcctga
     1261 ttttgtcgcc atctatgttc ttcattgctt ccgcaatttc tttctggatc ttctccatgt
     1321 caacattcag gtcggcatta tccagttcag tgagtacatc atccaggtcc cggactttct
     1381 tttccctgtc ttttgatttt ttaggaacag tatctgttgc cgattgctgg tattggccag
     1441 gagattgttt atagtcccat gatacaagac caatagtaag accgattgta agtaaaacaa
     1501 gtgctacccg cccggttgtg aaggttgttg gtttcataaa tgaaaagttt aatgaatgag
     1561 attaattatt atcacgaaat atttcgtatc aaatatacga ttgatttcgt attatattgt
     1621 cctgccggac atataaagaa aattttaaca aaaaagccat ccgctgatca gcggatggct
     1681 tttaatcagg tttatgggaa gcttagtttt ttctgaatgc ccagcggatc atttctttcc
     1741 aacttggttt tttcccgtac atcaggatgc cggtacggta aatttttcct gcaaaccaaa
     1801 ctgtaaaaat aaaacccagg atcagcaagc ccatagaaag agccagttgc cacacaggaa
     1861 ccgttccggg caccccatat ggtattctcg ccatcattac aatgggagag ctaaagggaa
     1921 taatacttcc ccatacagca ataggccctg ttgggtcaga aatggcagct gtcatgagtg
     1981 caatagaaat aattacaagc atggtgatag ggaaactcaa tgattgtgct tcccgcatgt
     2041 cttcgttcac cgcactgcca atagcaccgt ataaggatgc ataaaaaaag aagcctgcaa
     2101 ggaagtagaa accaaagcaa aaaaggatca atggtacgtt tacactggca aacatttttt
     2161 gcatactgcc aaccatttca gtggccatgc caccaccagc gccggaagct ttaccaacat
     2221 tatagacaat taaaacaaat gcaatccaca aaaagaactg ggttaacgcc accagcccga
     2281 tacccaaaat cttgcccagc atcatctgga aaggttttac ggaggataca atgacctctg
     2341 cgatcctgtt ggttttttct tccattactc ccatcattac ctgtgaacca tagatgagca
     2401 gtataaaata gataagaaaa cccgccacat agccaatccc ttcagccgtt tttacattgg
     2461 cattctcgtc tttaatattc ctggcagaaa ccctgagttt actttttacc agtattgttt
     2521 gtttttctgt atctatctgc aggctgtcat ttttaatttc attccatatc cggttaagtt
     2581 tagcttctac tgctgcagta gaaccgctgc catatgattt gttcgcttta aagaaaagac
     2641 tgtccgaacc ttctttccag ttgattgccg gtataataat atacccatca tagccttctg
     2701 cattatggtc tgctattatt ttgtccgtat tgctttttac aagtgtgagt acagaactgt
     2761 tatcagcaag gttttgttta ttgatcgcat cagtggagaa gtacccggac gaatcaatga
     2821 ccgctacatg gagattttgc ttgctttttt ctgcgatgaa tcccatcccg aatatcaaac
     2881 ccaggtacag caggggaaat agcagggttg aaatgataaa tgtttttttt ctgacccttg
     2941 tcaggtattc tctttttatg ataatccaga tcttattcat gatagttggt taaatggtaa
     3001 ttgaattatg cttccacttt ttgaaactgg cgggctgttg gggttccttc caccagtttg
     3061 ataaaaatat cattcaggga aggaaggatt tcattgaatg aatgaatagc tatgccctgc
     3121 tgcaataagt attgcaacac attattaggc gtttgtcctt cttttatcct tactacatga
     3181 gaatgatcag cagatcccac caattcgaaa ggggcgttcc cgttttgaga agaaagcatt
     3241 tgttctgtgc ctatactaaa aagattttct ttaaactcct gttttacctg cttcacggtt
     3301 ccgtccagta ttttctttcc tttattgacc aggacaatgt gattgcagat ttcttccacc
     3361 tgctccattc tgtgggtact aaagatgatg gtagaaccac tttttgccag gttatagatt
     3421 tcgtctttga tcaggttgct gtttaccggg tccaggccac tgaaaggttc gtccagaatg
     3481 atcaacttag gttcatgcag aacagtggta acaaattgga gtttctgtcc catccccttt
     3541 gacaggtctt ccactttttt gttccaccag ctttccattt caaatttgat gaaccatttt
     3601 ttgatcttcg ccatcgcatc cttatggctt aatcccttca attgcgccag gtacattgcc
     3661 tgttcgccga ttttcatttt tttgtacagt cctctttcct ccggcatata accaatctgc
     3721 ccgacgtcat tgacagggtc aaatttttta ccatcaaaaa tgatctgtcc ttcatcagga
     3781 tagaaaatgc cggtgatcat ccggatgagt gtggtcttac ctgcgccatt aggtcccagc
     3841 agtccgaata tctgtcctct ttctacattg aggctgatat catcaacagc tttttgggta
     3901 gcgaaatatt ttttcagatt ttgaagttcc aggattgaag gcatatagtt ggtttaaaat
     3961 gtaaaattag tagtccttgc gaaatgctgt gttttaatcg gtaagtggca taaatagaag
     4021 cgtgaaatgc atttataagg gtgcatcagg ttgctttaag tattgttatt atttgtttag
     4081 ctactttttc tccatccata gctgcactta caattccacc tgcatacccg gcaccttctc
     4141 cacaaggata tagattttta agttgtgggt gctgcaaact aactgcatct cttggtatgc
     4201 gaacaggaga ggaggttctg gattctgtgg ccaccactac cgcttcattg gtgtaataac
     4261 ccttcatctt cctgccataa tctttaaacg cctgttgcag gcttttatga atcaccgggg
     4321 gcaatacgcc tattagcgat gaggaatgaa tcccgggtaa ataagaacaa ccaggcaggg
     4381 aagaagacaa tctattttct gtaaaatcaa ctaatcgttg agcaggcgca acaaattttc
     4441 ctcctcctgc ctcaaaagcc ctctgttcta cagattgctg gaagaacatg gctgctaatg
     4501 gtccatgttg tttaaaaacc tgtatgtcct tttcttcaac ggtcactaca atgccgctgt
     4561 ttgcgtaggg attattccgt ttggaagggc tccagccatt tactaccagt tcaccgggag
     4621 aagtggcagc cggagcaata atgccaccgg gacacatgca gaaagagaaa actccctttc
     4681 catccacctg ttgtacccaa ctgtaagatg cagggggcaa aaaatcgcca cgaacctggc
     4741 aatggtattg agcactgtca atcaatgcct gtggatgttc aacccttaca cccaacgcaa
     4801 atggttttga ttcgatcaat aattttttgg aatacagtaa ttgaaaaata tctcttgcag
     4861 agtggccggt ggcaagaata aaagcgtcag cttcaaaaat attcccatct gctgttttaa
     4921 caccggttat tttatcctcc ttaataagga tatcaacaac ttttttttca aataaaaaat
     4981 cgccgccaca agccactatt ttttcccgca tggctgtgat aatatgcggc aatttgttcg
     5041 taccgatatg cggatgggat tcgtataaga tattttcttc tgccccaaaa tgtacaaaca
     5101 ggttaaggat gcggttaatg tctccccgct tgctgctccg ggtataaagt tttccatcac
     5161 tatatgtacc ggcacctcct tcgccaaagc aataattact ttcaggattt atcactcctt
     5221 ctttatttaa aatagccagg tctcttctcc gggctcttac gtcttttcct ctttcaagca
     5281 ggattggttt aattccggat tcaattaact gaagcgcagc aaataagcct gccgggccgg
     5341 ctccaactat aatgattgtt cgttcagcat tttttacatc tttaaaatca aatgacaaca
     5401 tttcccgttg ctgaaagggt tcattgatat aggctttcag ggaaagattg atccagggct
     5461 gcctgcccct ggcgtcaatg gactgtttta gtatagtaaa accacttatc gcaggtacct
     5521 ttattgcttc agcttttgcg atatattttt taatagcggt atcattggct gcttccgaag
     5581 gcagtaattg gagtgaaatt gtcttttgca tactgtcagg tttttatcct gaaaaatttg
     5641 atgtaaagat gcaaataaaa ttccgggata aaattctgcg atttcttgtt gtgataccaa
     5701 aatagtaata ccttcaaacg agatcaatag ttatgcattc aaggttattt tttccaacgg
     5761 ctctgctact tatttttatt gcaggtaatt cctgtaatac tacctaccag ggacaagccc
     5821 ttcagtacaa atcttaccgg attgctaata cgcagcaaca ggattcagcg ctactgactt
     5881 ttttgaaacc ctatagtgac agtgtaaaca acagtatgaa cggggttgtg ggcattgcgg
     5941 aaatgagtct ggataaaaag cagcctgaaa gtacgttggg taattttatg gtggatgcat
     6001 ttttgattat gtccagggaa aagtataaca cagaagtaga tgttgctttt gtaaactttg
     6061 gcggtatccg gttgacccaa ttacctgcag gcaatgttac aaggggtaaa atttttgagt
     6121 taatgccttt tgataattta ttaattcttc aaaaagtgaa aggcagtgtg ctgcagcaat
     6181 tacttgatct tacagctgac aggggaggct ggccaatggc cggattaacc atgcagataa
     6241 aagataaaaa agctgtcaat gtgatgatag gtggcaaacc gatagatcct gacaggttgt
     6301 atacgatcgc taattcagat tttatcgcca atggaggcga taacgctgac atgcttcgtc
     6361 caattccaca aataacgaat ggctacctga tgagggatgc tatttttgat tatatcagca
     6421 aattgaaaag ccagggtaaa aatatcactg caaatattga aaacagggta accaatgctc
     6481 agtagaaaaa agttcatacg acaggctgca atgtctgcag gggctatcat ggctggtccg
     6541 tcattgctgg aagcggcaac atatttcagc ccgttgaagt taaccatttt gcacacaaac
     6601 gatacgcaca gtcgtttaga gccatttcca atggatggag gccgcaacca gggactaggt
     6661 ggcgtagcag gccgggcaag attaataaac cagatcagga aggaggaaga acatgtatta
     6721 ttgctcgatg ccggagatat attccagggt acaccctatt ttaatatata taaaggtgaa
     6781 ccggaaataa gagcgatgtc aaccttaggc tatgatgcct gtactatagg taaccatgat
     6841 tttgatgcgg ggatggaaaa tttagcaaca caactaaccc gtcatgcaaa ttttccaatg
     6901 ctggtgtgca actatgattt ttcaggaaca ccgatggaat ataaaacaca accgtacaag
     6961 atttttaaaa aaggaaattt gaagataggt gtgtttgggg ttggtattga agggaagggg
     7021 ttaatccccg ataatttatt cggggccacc aagtatcttg acccggttca aaaggcaaat
     7081 gaagtggcat atgaattgaa gaagtttaaa tactgtgata tgattatttg tttatcacat
     7141 ctcggtaatc aatataaagg aaacgatatg gtcagtgatg aagtgctggc aaaacaatca
     7201 gaaaatattg acctgattat tggcgggcat acgcatactt ttttaaatga accgttggta
     7261 tacaaaaata aaatgggcag tgatgtggtg gtaaaccagg taggttttgc cggtattatt
     7321 ttaggtaggc ttgattttga attttccaaa ttttcgggca aaaaattagc aaaaagtcac
     7381 tcaatttctg tttcacaaaa atcaggggga taaaaatttt tttttagaaa agaaatggtg
     7441 atattcgcct ccctgtccaa ctttttttat tgacaaagtg ccaaggtttt tagtggacaa
     7501 actcaggaac aactatagtt aacacgacat aaactgctta ctgactgatg gagaaaaatg
     7561 ctgataaaat ctggacgaat tgtttaaaga tcatcaagga tattgttgaa tggcagcatt
     7621 acaagacctg gttcgaacct atcaagcctg tatcgctcaa aaataatgtt ctcgtcatcc
     7681 aggttcccag tcaatttttt ttcgaatatc tggaagaaca ttacgtaaat ttattagcga
     7741 agacattaaa aagagaattg ggcaaagatg cccatcttga ataccggata atggttgata
     7801 gtggtaacag caagaataaa cctgttacca tggatgtaag cggccaggga tttaaaactt
     7861 tttcaaataa cgaaatggat tttcctttag tcataaataa tcctgtaaag aatccgttcg
     7921 ttatcccggg tctgaagaag atgcagatcg acccgcagtt aaatcatatt tacacatttg
     7981 aatcttttat tgaaggggat tgtaaccggg tagcccgccg tgccggtaaa acagtggctg
     8041 aaaagccagg cactaattcc tttaatccat tagttatata tggaggggtt ggtttgggca
     8101 agacccacct ggcccaggca atcggaaatg aagttaaaag gctacacccc aataaagtag
     8161 tgctgtatgt aagttctgaa aagttcatca accagttcat ggatcatagc cggaataatg
     8221 cgataaacga ctttatacat ttctatcagc tgattgatgt tttaattatt gatgatgtcc
     8281 agttttttgc caaagctgaa aaatcgcagg atgctttctt tgctattttc aatcacctgc
     8341 atcaatcgca taaacagctg gtccttactt ctgacaaatc acccaaagac ctggaagggg
     8401 tacaggaaag attgttaagc cggttccgat ggggcttaag tgctgattta caggtgccgg
     8461 aatacgatac ccgtatagag atcctggaga aaaaaatgaa gaatgacggg ttggaaatgc
     8521 ctaaagaagt ggtaaagtat attgcatata atataagtaa taacgtaaga gagctggagg
     8581 gtgccctgat ttcactgttg gcacaatctt cgctgaacag gagggaaata gacctagact
     8641 tagcgaaaaa agtgttacgg aattttgtaa aatcatccag caaggaaatt accatcgata
     8701 ccattcagaa aatggtatgt gagtactttg atgtgcccta tgataagctt ttgcagaaaa
     8761 cccgcaaaag agagatcgtt caggcaaggc agatcactat gtacctggca aaggcattta
     8821 ccaagaactc gttgaagacc attggtgaac attttggcgg ccgtgaccat actactgtta
     8881 tccattcctg ccagacggtg aaggacctga tggatacaga tggtgttttc agagaaaatg
     8941 tattggaact acagcagaaa gtgcaactgg ctgctatgta aaataactac aggacggttt
     9001 caaggatatt gaaacaaaag cacccggccg aaaagtcggg ttttttattg ccagaaaaga
     9061 aaaactgtat tgtggggaaa gcaattaatc tttatttttg cttccccttg gataaaatgt
     9121 gttggagagg tgcctgagtg gccgaaaggg ccggtttgct aaaccgttgt acggacttaa
     9181 atctgtaccg agggttcgaa tccctccctc tccgcaaaag cccggtttca ccagattgaa
     9241 gccgggcttt ttttataaac atatcagtta taatgcataa ggaaattaaa tagcagcagc
     9301 aatttttctt catatatttt ttttatccct cgcaatacaa ttttattgtg ataattcttg
     9361 tagtatttaa tcgatttttc cggtaagtgg agaaaatttc aactcatatt ttgatattta
     9421 tcaactttaa aaactgataa attttattaa tgctcaattt ttgcatttaa ggatattaaa
     9481 tgataaattt gattcgcagt aaatgttaat ttttacatca gacaggtaat tacttttctt
     9541 tcaactttcg gcttttagta gataacagac tgcacgatta gtagcatgca ataacttcaa
     9601 ttgtccaagg catttgttgt gttgttctgc accgattacc tccccactta gaatagtagt
     9661 atctacatta tcctgagaaa aaactcatat taatctccgt gtccgtttga atttgtgtac
     9721 caggttgctg gcacagcctt atttattttg ttttattact acaaaccaaa catgctgaaa
     9781 tgagaaagtt caaagtatca acatttaccc ggtttttact ggttactgct tttttaatta
     9841 ctatgtctta ttctgagagt aaagcccagt tggacataag tataggtact ggtacaacgg
     9901 gaaatactac aactacttat ccatgccctt tacaggattt ttatgaagga agcaggatgc
     9961 aatttctgta cagggcttcc gaattaaatg ctgcaggcat ggcccccgga ttaataagtt
    10021 ctataaaata taatgttgtt tctcttggta ctgccggttt agttgaaggc atggtaataa
    10081 gaattgggca tactactgtt accacactct ctacatctgc atgggatcca ttttccggag
    10141 tacctgtttc cacggtttcg accgactacc agccagtaaa tggaattaat acgtttaatt
    10201 tccctgttcc ctttagctgg aatggttcag ataatatttt aatagaaata tgtaatggag
    10261 aacccgggaa tgcaactggc acatggttta cttctaatcc ggtaattccc tggacgacag
    10321 accttacatt taacggctct cataattaca gggcagataa tcttggaaat ctatgtagca
    10381 gtgcgacaac aacaaatact ggtactcaga ctacaagacc aaacattaca ttctcatgga
    10441 cacctgctat tgcttgttct ggaactccaa ctggaggtac agctgcggcg aatccatcta
    10501 cggtttgttc aggtcaaaat tttgatttaa gtcttagtgg ctttacaatt gccagcggaa
    10561 taacttatca gtggcaatca tctgcagata attcagtatg gtctgatatt acaggtgcaa
    10621 cttcttcatt tcttactaca tcccaaacag tcactacata ttatcgctgc attataacct
    10681 gttcaaacag cggtctttct gctaactcaa gcagtgtgtt ggtaacttct ccagcattgg
    10741 tatcaggaac ttttacgatc aatagcgctt tgccaacaag cggtaccaac ttccagtctt
    10801 ttaatgccgc atataattat atatcatgtg gtataagcgg gccggtagta tttaatgtgg
    10861 atccagcctc gggtccatac acagaacaac tgataattaa cccggttccg ggagcttcgt
    10921 caacgaatac cattacattt aatggtaacg gaagagtact gcagtttact tccaccaaca
    10981 ctaatgaaag aggtgttatc aagcttaacg gggcagatca ttttgttttt gacagcctta
    11041 ctattaatgc tacagctacc acaactacag aatacggttt tgctgttcaa ttattgaaca
    11101 acgccgattc caacattata agaaaatgta taattaatgt aactgatgca cagacttcta
    11161 ctaatcatgt gggtatctcc atcagtgcca gcgccacctc agccaccggt accggcaatg
    11221 ctgagtgtga cgataacctg tttgagttga acaccattac cggtggttat tacggaatta
    11281 ccaatgtggg cagcactacg gtggctaacc agcgcaataa gtttatccgg aatatcatta
    11341 aagattatta cctgtatggt atttatacca gtggcacttt cacttcttta atagagggca
    11401 atgattttag tcgcccggcc cgtgccaata ttggtacagg aacttcttat gctatttatt
    11461 tcacttcact tagcaccagt gcaagagtga atggaaacta tattcatggt ttgctggatg
    11521 tgaacaccgc cagtacgaat gatgtgtatg gtatttattt taccggggtg gatgctattg
    11581 caggtctgga gaatatcgta agcaacaatg ctatctatga cataaaaagt aatggtactg
    11641 tctatggtat ttataatgcc agttcagata atatattcta ctatcataat actatttcac
    11701 tggatgacgt ggcgagtacc actacggaag tgacccgtgg tttttatcag acaactgcgg
    11761 cagcaggaat agaatttaag aacaatctca ttactattcg caggggaggc acaggccaga
    11821 tgcatggtct gttttttaat actaccactt ccacaattgt ttcgaataac aacaacgtat
    11881 tttttactcc ggcagctaac gttttttatg gttcatgggg aacaaccggc caggcaactc
    11941 tggccaactg gcaaacggct tcttcacagg atgcgaattc agcatatata gatcctgcct
    12001 atactaacct tggtttgggt aatctgaaac caacgtctgc cggaatgaat gacctgggtt
    12061 cagcagttgg tattacagct gatatccttg gtgtatcaag aaatgccacc actcccgata
    12121 tcggggcatg ggaatttgat gtcggaggtt gtactgcacc tccaaatgcc ggtgatgcca
    12181 ctgcaagtat cagtgtaccg atttgtccca atgagctggt tgcactaggt cttgtaaata
    12241 atacttttgg aaccggccaa acctatcaat ggcaaacagc caccacactg gctggtccat
    12301 ggaccaatat tgggccttcg cagaataacc cggcctatag tgtcaaccca acggttactt
    12361 tatattaccg ttgtgcggtt acctgtagcg gatcaaccac tttctctacc cctgtacagg
    12421 tagtggtaaa tgcattgttc cctgccggaa cttatactat caatagtgct ttgccaacag
    12481 gtggtaccaa cttccagagt ttcaatgatg ctgtttttgc tttgcgttgc ggtattggtg
    12541 gtcccatcgt atttaatgtt gtaagcggat ctggtcctta caatgaacaa gtgattatcg
    12601 ggcagattca aggttcttct gctatcaaca ctgttacttt taacgggaat ggcgaaacta
    12661 tcactaatct ttcaacgaat acgaatgaaa gagctgtcat aaaactgaac ggaactgatc
    12721 attttgtatt tgatagttta acggtggtgc cgcagggcgc tgctactaca gagtatggtt
    12781 ttggattcca tttgctgaat agtgctgatt ccaacattat ccgcaattgc gatattaaca
    12841 tgagcaccac tactacgtct accaattttg cgggtatagt ctccagtgtg ggtacaactg
    12901 ctacaacaac cggaactaat ctgtcggatt ataacctgtt tgaaaataac gagatcaccg
    12961 gtggttatta tggtatcact tcagtgggaa gtaccacttc ggctgttggt aataatgcga
    13021 tggtcagaaa taccattaaa gatttttatg cctacggtat ttatttatcg ggcaatttta
    13081 atgcactggt tgagaagaac aatatattcc ggcctgccag gaccgcttct actaccactt
    13141 atggtatcta tgtcacttcg ctgaatacag gggttaaatt atccaagaac aggatacaca
    13201 acctgtttga tggcgcacct actgctacga gtacttctta tggtatttat tttaccggcg
    13261 tagatgcaat tgcgggcctg gagaatattg tcagcaataa tgcgatctat aatatgaatg
    13321 gtaatggcgc ccagtatggt atctacaaca ccagttcaga taatgtattc tattattata
    13381 atacagtgaa cctggatgat gccaccgcca ctaccaccag tgatgcttat tgcttctacc
    13441 agacaacaga agcggcaggt attgatctga agaataacct atttagtatg acaagaggcg
    13501 gtaatggcga taaattctgt atgtattttg ccacggccgc tactacattt acatctgatt
    13561 ataatggcgt attcaggaat gcagttaatt cacatgttgg ctataatggt tctaaccaga
    13621 caacattggc caactggcag gctgctacta cgcaagatgg caattctgta tcggctaatc
    13681 catattatac ggatgccgct gccggaaact tggaaccaag aacacctgca ttggataacg
    13741 aaggaactcc tgtaaccgga attacttcag atatccgtga tgcagtgcgt agtgccacca
    13801 caccggatat cggtgcatgg gagtttgcag taccagtttg tacagcaccc ccaacagccg
    13861 gttcagcaac agctgttccg aataccggta tttgtttggg taccccgatt gaccttgact
    13921 taaatggcaa ctctattggc tccggtcaaa gttaccaatg gcaatttgcc acagctgctg
    13981 ctgggccgta tactaacctg ggtaatccaa agctgttccc ggatacaaca attctcgcca
    14041 gcggtacttt ctggtatcgt tgtgttgtga cctgtagttc acagtcggct aactcaacgc
    14101 cggtgcaggt aaatatgaat cctcctttcc cggctggtgt atatacaatc aataatacca
    14161 taccgaccga ttacccggga ggcagcaatt tcaattcatt tgtagaagca gtgtcagtaa
    14221 tggagtgtgg tatttccggg ccagttactt tcaatgtagc agcgggtact tattccgagc
    14281 aggtgagaat gcatcggatc accggcgctt ctaatacaag ccgtgttact ttccagagtg
    14341 ctaatggtaa tcctgcttct gttattttaa cttatgatgc gaccaatgca gcagctaatt
    14401 atgtattaca gttagacagc gccagttata ttacttaccg gaacatgacg atcactgcaa
    14461 tcaatgccac gaacggaagg gcagtggata tagccaattt agcatctaat gatagtttgg
    14521 taaataatat cattaatgca ccaactgcta ccggtactgg tacaacagtg gtgggtattc
    14581 atgcggctaa tcttgccggt ggtgatcata cgatcaaagg caatacagtg aatggtggtg
    14641 tgacaggtat ctattttggt tctaccacta cttcgccaag tgcttacaac tttatagata
    14701 gtaatacggt gactgggtca tattattatg gtatttataa ttcaaataca cggtttacct
    14761 ctgtaagcaa gaataccgtt acagtagctg ctccaagaaa taccactact tatggtattt
    14821 acctgaccag tgctgattca gcttaccagg taaataataa taaggttgat atcaacggga
    14881 ccacttcaac tacatatggt atttatctta cgggatgtgg tgccaccggt cataccggaa
    14941 gtgtgtcagg taataaaata acaggactta caggtataac cggaacattg tatggattat
    15001 accagacttc ttcaattggt aactatacaa agaataacgt tattaatatc aacaccagtg
    15061 ctacgacagc ttacggtctg tattctacag gtggcaatgg tatcaaatat tataataatt
    15121 ctgtgcagaa tgcatctcct gctgcgggta ctacaaatat cgcagcatat ataagtcata
    15181 ctacttcatc tgccggagta ggagatatta gaaacaatat cttctcgcat actgccaatg
    15241 gaatagcggc taattatttg aatgtagcaa atatttatac cgactttaat atgttctata
    15301 cagctggtgc aacattgatc aggcagaaca cagtaaacta tgctacgtta caggattgga
    15361 agaacggcca gaactgggat tataattcta ttgtatacac tcctgccttt gctgcgggta
    15421 atgaattaca acctgatgtt actaaccctg atgtatgggc tatgcatggc cgtggtgttc
    15481 agataactga taatgcaaca gatattaatg gtaatataag acctacaact ttgacggcag
    15541 gtgttcctga tatgggagct tatgagttcc tgccaactgt acagccgcct gtattgcctg
    15601 ccacacctgc tgctcctgct gcaggtacca cgcaggtatt tatgtttggt acagatacag
    15661 tgcaaaaaat aacatgggcg cctgcatcta ctgttccaac aactgtggat gtgagaagat
    15721 actcaggtat cattcctccg ggtttggcac ctggtcagca atccatgtat tattatactg
    15781 atgttgatat caccggaagt gcaccaacca atttcacaaa tcagcaattc tatatagatc
    15841 catggatgag ggatatacca tcagaaccaa ctgtaaaatt gggccgcact aatacagcag
    15901 gcacctggtt ggttgcatcc ggaagtgtgg tagatgacct gggtaatgtg atcacagaaa
    15961 gcaatctcag tttccttgat aaatttaccg gcatgacaga tggcgtagca cctccaccag
    16021 ccgtacctcc tgtgtttaca ctggatactt ctaacagggg caaacgattc tgggtgggtt
    16081 atggccacca ctatggattc agcagcaact cacaggacat ggcactttac ctgagtgcag
    16141 aagattcagc caacgttacc gtgaaagtaa acggaacgaa ctgggtacgt acttatgcta
    16201 tccctgcgaa tacagtaaga gtgagtgact ttatgccaac atcaggactg gtggatgcaa
    16261 gattaacaga tgaaggactc ttcaataaag gtatcagtat tgaaagtgat gtgcctattg
    16321 ttgcgtatgc tcatatcagt gacggagcca attccggcgc cggtatgttg ttgccaaccg
    16381 gtgtatatgg ttatgaatac agatcactga actcccgaca gtactatcca actggtggtg
    16441 caggttctta ctcctggatg tttgcgattg cagatcatga tagtacattg gttgaaataa
    16501 ctccgtctgt tacaacgaaa ggtggtcgcc cagccggtgt accgttccag gtatacctga
    16561 agagagggga agtgtataac gtaatgggta ctcaagccac cggtggacag ggaactgatc
    16621 tttccggttc aacaatcaaa tcaattccga atgccagtgg taaatgttac cctatagcag
    16681 ttttctccgg cagcagccgt actgctatct gtaatacaac caatggtgat aatatgatac
    16741 aacaggtatt cccggccagc gcatggggaa ccaagtacct gacttttgcc accgccaatt
    16801 ctgcttccaa taccaattac aattccaatg gctggagagt gatggtaaaa gatccggcta
    16861 cagtggtaag gagaaatggt gtgatacttg accctgctac tttggtagtg ccaggtaatt
    16921 attatgagtt cttcgttgga aataataccg gtgctaatac agccagcttt atagaggctg
    16981 accaaccggt tatggtagca cagtatatgc tgtctaccag tggaacaggt tgttcaggtg
    17041 tacctgcacc aactggtaat ggtgatcctg aaatgatcta tatcagcccg atagaacagg
    17101 gtatcaaaaa agcaaggttc tataacacca acaataattc gatcacttcc aattatgtga
    17161 atgttatatt accgacagct gcattgccaa gtttgcagat agatggcagc actacattta
    17221 cagatgtgtt tgcgcatcct aacctggcag gttatacttg tgtaagacaa aactttgccg
    17281 gagcagcagc tcagcattat attacctgtg actctgcatt tactgcaatt acatatggat
    17341 tgggtagcgt ggagagctat ggttataatg ccggtacact ggtgaaaaat ctcagcgcag
    17401 ttccttcgtt caataatgtg tttaatacaa gtggtactaa cacttatacc tgtaagggaa
    17461 caccattccg cctgagcctg cagatctctg taaaaccaac acaactggta tggcagctga
    17521 gccaggtgaa taatgtaacg ccaaatgtca atgtaacaca ggtgaaccct gtgcctactg
    17581 actcagtaac ggtgaacggt aagaagttct atatctatac actggcccag cagtatactt
    17641 tcagccaggt aggtacttat aatgtaccaa tcaccgtgtt ccatccaagt attgaaggtt
    17701 gcggtggttc agtagagatc gcattacctg taactgtaat tgcagcacca gtttccaatt
    17761 tcactaccaa ttttagcggt tgtgtaggtg atgcagtaca attcaatgct acaacaacaa
    17821 cgagcaatgg tgtaccgacc aatcaatgga catgggcatt tggtgacggt aacaatggtg
    17881 ccggtcagaa cccggttcat acctatgcaa cagcaggaac atacaatgta tcactgagag
    17941 gtattgctga tgacggttgt atcggcgata caaccaaacc ggttgtggtg aatccaaaac
    18001 caactggtgc catcacgcag gatacggtgt atgtatgtaa taacgccagc gccaccttta
    18061 cggtacaaaa ccctgtaacg ggtgatacct acaattggta tagcgccgca accgggggtt
    18121 cattactggg aacaggatca acttttgttt tgaataatgt taccggtttt gtgaatgtat
    18181 acctcgaagt gataagactg ggttgcgcca gtgctacccg tgacagggcc actgctgcta
    18241 tattgccaac attaccggca cctgttgtaa ctgtagattc tgtaagcacc aatacaatag
    18301 tattcagatg gacagccgtt acaggtgctg ccagctacca ggtatctact aatgccggtg
    18361 gtacatggtc aacaccatca tctggtccgc aggggttgac gcatacgata acaggtctgc
    18421 ctttgggaac ttctgttact ctcctggtga gggcactggg tggttgttta ccggcggtct
    18481 cacagcctgc tacaggacaa acagtaacag accaggtata tattccgaac agctttactc
    18541 caaataatga cgggctgaat gatgtgctgc gggtgtatag taatgtgatc cgcagtatga
    18601 agtttgtagt gttcaaccag tggggtgaga agatttttga atcaaacagc cagtcggtgg
    18661 catgggatgg tacacataaa ggaaaaccac aaccatcagg agtatatatc tatgtatgtg
    18721 atattgtcct gaacactggt gaaaggatac agcgcaaagg atcgatcaac ctggtgagat
    18781 aaaatacaag atcaacgtac cgtattaaat gaaaaggaaa acacaaatac tattcttcac
    18841 tgccagcctg ttattgacag gctggttaga agggtattgt caaaacatta caaataaagg
    18901 cagagatttt tgggttggtt atggacatca tcagttcatg gagcctggtc aatccaatgg
    18961 acaggatatg attttgtatt tcagtgcgga gcaggctgct aatgtagtag taacagtaca
    19021 aggcagaaca gcagtacaaa cattaaatta tgcggtacct gcaaatacag ttatatcatc
    19081 tgcagtaatg ccgaaagccg gaccaaatga ttgtcgttta tacgatgttc ctccaacttt
    19141 cggtggaaat ggaggagagg gattatttgg catgtctatt catatcgtga gtgatgtgcc
    19201 tattgttgcc tatgcacata tttttgggag tgtgtcttca ggggctacaa tgctgatgcc
    19261 tgttgaatca tggggttatt cttatatgtc aataaatagc cagcaaatag atgcaggcgg
    19321 tcctggtttt tcctgggttt atgcaattgc tcaacatgat aacacagtaa ttcaagttac
    19381 tccatctgtt acaaccagat taggtaaacc tgccggtgta ccttttaata ttacactcca
    19441 aagagggcag atatatcaat ttattggtca gtcagatgca ggtggtaatg gaaaccagtt
    19501 tactggctct acagtaaaat cgattgcaaa tgctgccggt gattgttatc caattgccgt
    19561 tttttccggg agcagcagaa caaagggaga acaagtgcca tgtgggtcaa gctcaggcag
    19621 ggataatgat atgcaacaat gctttccaac ccaggcttgg ggtaaaagat atctaactgc
    19681 gccattttca acttcttccg ggccttcaac agctgtaata ttgaatgcca gcacatttca
    19741 aacaagtgtc tacaaagttg cagtaaaaga tccaacaaca gtagttagaa gaaacggaat
    19801 tattctaact ggtcttatta ataacagtta ttatcagttt tcaagtaata ctgctgatta
    19861 tattgaatct gataaaccca taatggttgc acaatttatg tctggagctg caggatgcaa
    19921 ctccggaaca tggggcgatc ctgaaatggt ttttttaagt cctgtcgaac aagcaataaa
    19981 aagggttggt ttttacagaa acacaagaca atcaatttat gcaaattatg taacagtaat
    20041 tattccaacg gctggtgtat catcacttag aatcgatggg tcggctacat ttaatcatac
    20101 atatactcat ccgaatttac cgggatattc aattgttgta aaaggatggc aggcagcaca
    20161 ggctcaatgc cagatgtata gcgattctgc ttttacagca atcacttatg gtttaggaag
    20221 tgctgaaagt tatgcataca atgccggtac ctatctaaat aatctgaatg caattagtca
    20281 cacacaaaat acacctgatc cgactgttcc tcaacaccct tatacttgtg tcgggacacc
    20341 aattaaactt tctgtgttaa tggcttatca gccaacccgg ttagattggt ttttaagtca
    20401 gcttggcgcc aatattattc ctaatacaga cattattgat aatgccccgg tttcctccgg
    20461 tacagttgta gtaaacggaa ttacttatta taagtatact ttacccggta cttatatttt
    20521 caatgccgct gatacatttg atttgcctgt aaatggttat cacccaagta tcgaaaattg
    20581 ttataacaga gagaatctta ctatatcatt tattgtgaag cctaaaccac tggctgactt
    20641 tacgtttagt cataccgggt gtacattgga tcctgttaac ttttctggtg tggcttccac
    20701 cagtaacggt tatactattc gtgattacaa ctggacattt cctggtcctg ctactgctac
    20761 agggcaaaac gtaatcagga tattaccacc aggtatacat gacatcaatc tttccgttgt
    20821 ttccaatgaa ggttgtgcca gtgataccat gaaacgtatt acgatttatg ataaaccacc
    20881 gggtgatttt atggtaactc ctgctaattt atgcgaaggc agccagttta ctattaccga
    20941 tacatcatcc gcagcaatag ctgtaaatga atggtactgg gattttggta atggtgttac
    21001 gcaggttgtt acaaatggtc catctgtaac ttacacttat cccgcatttg gtacttatac
    21061 aattaaacat gtggcccgtt ccagcgccag ttgtgtaagt gatacggtga gcagggtagt
    21121 gaccgtttat gccaaacctg cagtctcgtt taccaatgat gcagccggtt gtttgtctcc
    21181 ggcaggattg gtacagttta ccggtactgc tacaacccct gacgggcaaa ctataagtac
    21241 ctatgcctgg aactttggtg atccaaatac aacacccggc aatccgaaca cttcggtact
    21301 gcaaaacccg acacataatt accagcaggg aacctatacg atcaatttca gtgcgacaac
    21361 tgctaatggc tgtacagaga atacaacaca aacattcacc tttaatttaa aacctgctct
    21421 cacttatcca ttgctgaatg cagtttgtga aaatgcgcca gcctattcta ttgcgaatgc
    21481 gactgttaca aatggtgtaa caggtacagg tatttacgag gggccgggta ctactgctgc
    21541 aggtctgttc acaccagcag ccgccggcta tggcacacat actatctggt acaagcttac
    21601 ttccggtgcc ggttgtattg attctgtttc acaaaccata ctggtgcatg caagaccgag
    21661 aacaaacttt acaatcccgg ccagtggttg tttacctgct aacggacagg tacagtttac
    21721 caacagcagt actattcctg atgcacaaac catgacatgg ttgtggaatt tcggtgaccc
    21781 gaatgcgaca ccaggaaatc caaacacatc aacagcacaa aatccggcac ataactattt
    21841 ggaaggaacc tatacgatca atttactggc aacttccgct aacggctgtg tgaaggatac
    21901 aaatatcact accagttttg ctgtaacacc attactttct tatgctgcat tacctgcagt
    21961 atgtgaaaat gcagcagctg tttccgtggc taccgccaac gttacaaatg gtgtggcggg
    22021 tacaggtgta tatagtggtc cgggtactga tgcagcaggt atgtttaatc ctgctatagc
    22081 tgggtatgga atacatacga tcacttatac tttcacgtca accggtggat gtgtacaaac
    22141 aaccacacag aacattcagg tgcatgcaag accgagaacc agctttacat tacctgttgc
    22201 aggttgcttg cctactacag gacaagtaca attcaccaat accagtacca tacctgatgc
    22261 gcaaaccatg acatggttgt ggaattttaa tgatcccaat gcaggtggag ctaatccaaa
    22321 tacatcaaca gcacaaaatc caacacataa ctatttggaa ggtacctata cgatcaattt
    22381 acaagcaact tcctctaatg gatgtgtgaa ggatacgaat attacaacca cttttgcggt
    22441 aacaccggca ttatcgttcc cggcattgac aaatgtatgc cagaacccgg gtgcagcacc
    22501 atcttccatt gcatcagcat cagcaacgaa tggtgtaaca ggaaccggtg tatacattgg
    22561 tcccggagta aatgccgccg ggttgtttga ccccaatgtg gcaggcccgg gtgtacatac
    22621 cattacctat acttttacta ctgctggcgg atgtaccggt acggtgaata atacaattga
    22681 agtttacccg aggccaacag cctcctttac tgccagtggt aataacggcg cttgcctggg
    22741 acaggcagtt acattcacga atacatctgc tgttccatcc ggaaccatca ctacctggaa
    22801 ctggaatctt ggggatggca atactccatc ttataccaat ggtaatccat ttacggttaa
    22861 ctatacatcg gccaacacgt acaatgtgca gttagtaaca gtgagtgaca ggggatgctt
    22921 tagtgaacct gtttctcaaa caatccggat aagcccgttg ccggtggcag actttactgt
    22981 acctaccggt atttgtatgc cgggtggcac tgctgctttc actaacctta ctgcggtagc
    23041 tgataattct gcattgacac atgtatggaa ttttggtgat ggtaatacat caactctcac
    23101 taatccttca catgtatacg ccgcatctgg taattacaat gtaacattag tatcgacttc
    23161 tgctttcgga tgtgtgcagc aggttactaa accaatgaat agtttctttg ataaaccact
    23221 ggcttcattc agtgtatcac cacaggagct ttgccagggt gcagataatg tgttcaccga
    23281 tggcagttcc gcaccaaaca gcaccataca atcatgggca tggaactttg ctgacggcgg
    23341 tacatcttct gcaagcaatc ctaccaaacg tttcaatacg ccgggagtgt ataatgtaag
    23401 gctgacagta acgaatgcag tgggttgtgt ttcagaacct tttgtattgc cggttacggt
    23461 acacctgcag ccggtaattg atgcgggcca gtcttatgta ctgccacagg gaactgtatt
    23521 gcaattgaat gccactgcaa attcatctaa cctggttttc aactggtctc ctgggtttgg
    23581 attaagcagt acttcaatac tgaacccgac actgacagcc ctccaggatc aggtatatac
    23641 actcacagca acaggagatt ttggttgtac agctactgat cagatgacag tgaaaattct
    23701 gaagcaggta aaagtgccga atgtattctc acccaatgga gataatatac atgaccagtg
    23761 gatgatacct aaccttggtg actacccggg ttgtaccgta gaagtattta atcgctatgg
    23821 tcagcaggta tatttttctc ctggctatgg taatccgtgg aatggaacgt acaaaggaaa
    23881 agaactgcct gcgggcactt attattatat cattcagttg caaaatggat tcaagccgct
    23941 gagcggatcg gtaacgatca tccggtagtt aaaacagcaa ctgaagacag gtaaaatttt
    24001 taatgaacca aaattaaatg tgatgcgaaa gagcttacga atttcattgt tgatgctgtc
    24061 aattggcctt gcagttagta aagtaaatgc acaggttgat ccgcatttct cacagtacta
    24121 tgtgtatccg tcctggctta atcctgcgtt gacaggtgct tttgacggac agtacagggt
    24181 ttctggtgta taccgcaacc agtggggcaa tatttccagt ccgtttacaa cgtatggcgc
    24241 cagtgctgaa ttcacaacag aaaaaaagat caacctgggt gtaagtgtct tgaaacaaac
    24301 tgccggagac ggaggatatg gttataccac tgcgtatgga aacattgcct atacaggtgt
    24361 tcgatttggc aaaaatgaag aacaccgtgt ggtatttggt atgcaagctg gtatcattca
    24421 acgtcgtttt gaccgcaata agatgacatt tggagaccaa tggaatcctg tttttggata
    24481 taatcccggt aatccatcgt ctgatgtact caacagaact tcagccaccg catttgatat
    24541 gggcgctggt gttttatatt ttgatgcgca acccggtaag aaagctaacc tgtatgcagg
    24601 tttctctgca tcacatctca ccaaaccgga agataagttc agtgccagcg gagatgaaaa
    24661 atttccgatg cgatatactg cacatgcagg tatacggttc actttatctg acaggttcag
    24721 catcacacct aattttcttt atttatcaca aggtaccgca acggaaaaaa tggcaggtgc
    24781 atacgggcaa tacaaagcta cttcagatgt taacctgctg atcggcgcca actatcgttt
    24841 tgaagatgcg atatcacctt tctttggatt tacctataag aatatgatgc tgggtgccag
    24901 ttatgatatt aactcttcag atcttggaaa gctggtgaat ggatccaata gttttgagat
    24961 ttccctgtct atcattggaa agaaatcaac caaaacaccg gaggtggaat ttgtttgtcc
    25021 gagattataa tatttgaaac cgaattttaa ttactgcaac caactagtat gaaaaaaata
    25081 tttgttctaa cggctgttct tttttctttc gcttcgttgc atgcacaatt tgtgtatgat
    25141 tacctgaaag ctgctgatga ttattttcag aaaggcgact atgcgtctgc tgctgaatac
    25201 tatgaaaaat atatgggtgg tgataagacc ggcaaagaca aagagtttaa accctacact
    25261 ccccagaatt catctaagaa aacagctgcg gctgccgtca acaaggagca ggcaatgtat
    25321 aacctggcag aatgttatcg tttgctcaac tatccatcca aggctgagcc tcagtataaa
    25381 gaagtgatgg tggcgaataa aaaacaattt ccgctggcgc aatatcatta tgcgtcacag
    25441 ctaagagctt tgggaaaata tgcggaggct gagcaaaatt ttaaaacatt cttatcagga
    25501 tatccggcaa atgatgagta taagaaaaat gcagagagag aattgaagaa cctgcaattc
    25561 atacaggcag agcttgttaa aaaagacctg aagtattata ctattaataa agcaggtgga
    25621 gaattgaatg caaaaggcgc cagttatgca ccagcctggt tgaattcaaa tacattgtta
    25681 tttacatcaa caaggccgca ggacactacc gccaaaacca tggtatacac caatcgtgtt
    25741 taccaggctg tgtacgatgc agggaccctt gccagtataa gcccggctac tattccccag
    25801 gaaaaaggga tgcaacaggg agtggtaacg gtgacgcctg atggcaacac catgtacctg
    25861 accaaatggg gtatttccgg tgataaaaaa tcagccgcta tctattccag cagtaaaacc
    25921 aatgatggat ggagtgaacc tgttaaactc ggtgaatcga tcaatgcaaa cggagctaat
    25981 acacaacaac cttttgttac agctgatggc aaatatcttt tattctccag taacaggaca
    26041 gggggacaag ggggatatga tctatggtat gcaccattgg agaatggaac agccggtact
    26101 ccggtcaaca tgggtgctgc tatcaatacc agctatgatg agcaggcacc tgcatatcat
    26161 gaagcatctg cttcgcttat cttttcttca aacggtagag tggggatggg aggatatgat
    26221 ttcttccgca gtaagggaaa tatcggtaac tggactgaac cggtgaatat gggctatcct
    26281 gtcaattcag tgaaagatga tatttacatg gtgagcaggg gatctgctaa aaataatgta
    26341 ctggaaaatg taatgctgag ctctgacagg gatgcagtgt gctgtctcga gctatttgca
    26401 ttacagaaag taagaccact caggcaactg agcggccgtg tgctttcctg cgatccttcc
    26461 aaaccattga gtggagctac tgtaaccatt gttgatccgg ccagtaataa aacaatttat
    26521 acaaaggaag tgggagctga cggtacctat tcctttacta tggaagatca ccagccgttg
    26581 aaagtgcagg ccgaagctaa aggctttata agcaatgctg ccaatgttgg tacaccggct
    26641 gacctggaag aagaaagtat gaggtatcct gatctttgcc tggtgccaga agcgccaaaa
    26701 gtggatgaga aatttgttat agagaatgta tattatgact tcaataaagc tgacctgaag
    26761 ccggaatcat acccggcatt ggatgaagtg gttaggatgc tgaatacata tccaaatatg
    26821 gccattgaac tgggtgcaca taccgatagt aaaggttctg acacttataa tatgaaactg
    26881 tctgaggcaa gagcacaaag tgtggtgaaa tacctggtgg aaaaaggaat ttcggctgaa
    26941 agattagttg caaaaggata tggtgaagca atgccgatag ctccaaatga aattgatggt
    27001 aaagacaatc ctgaaggaag agagaaaaac aggagaactg aattcaaagt attaaagaac
    27061 gagtaaaaac caactatagc aggaataggc cccgttttta aaatccgttt actgatctga
    27121 aagcgaaaca gcaatgaaga aatatatttt tcttgtgata atgattgtgt tgggtggtat
    27181 ggcaacattg ccagttttag cgcagcagaa accacattac acccaataca tccttaacca
    27241 gtatattgtc aacccggcat tgacgggaat cgagaattat actgatttga aagttagcca
    27301 ccgtcaccag tgggtaggta tccaggatgc acctattacc tcttatcttt cagttcatgg
    27361 accaattggt aaatcagact atcgtaaaac agctacttca tttgatttaa aaggggagaa
    27421 tccccgtggt aagagatact gggaagaata tacagccccg gaaccccatc atggtatcgg
    27481 attgcaggtg atcaatgata ttacagggcc gttgagtaat ttttcccttt atggcactta
    27541 cgcttatcac ctggctcttt ctgcccgtac cagcctgtcc gccggttttg gggtcggatt
    27601 taatcgtatt agtctcaatg ctgataaact cgattttggc gatgtaatag ccgatcctgc
    27661 tgtgtttact tcaggtatcc tgaacaaaac aagactggat atgatggctg gtttgtacct
    27721 gtattctgct gattatttta ttggcgtttc tgcacagcag gtagtgccac aaaaaattga
    27781 tttctccaat gggtatatcc ggccaaaaga aggcagaaca gtgccccatc tttttggaac
    27841 tgccgggtat cgcttttcgc tgggcgataa ttttaacctg ataccatccc tgatggtgaa
    27901 atatgtagat cctttgccgg tacaggtaga gggtaatctt aaattgcagt atctcgacag
    27961 gttatggatc gggggtagtt acaggcacaa agatggctat gccggcatga tgggtctgaa
    28021 tgtctctaat atttttcatg ttgggtattc ctatgattat accacatccc gtttgaataa
    28081 tttcagtaaa gggactcatg aggttttgct gggctttgtt attggtgggt acccggataa
    28141 ttgcccgcgg aatgtctggt gattattaat ttccctttac caattatgat taagtaatct
    28201 gtggtgacaa tgcctgttac ctggcaatat accgttcaaa gggtattatc taaattcaaa
    28261 aagggtggta tattcgatta taaattaaaa tgaacaatat gaagaggtac ctgattttaa
    28321 caactgtttt gtttattgca ggcagtatat ctgcacaaaa atttcctaaa ccgggttgca
    28381 gtaccgtttt ggcagaagca atgttttccg aagtggatac aacgaaacca aggggagtaa
    28441 gtgataatta ccatacctgg gaaaatggcg ctgttctttt ggtgaaattt atgcctggtg
    28501 gtagtaaaat gctgagagat aaagtaatac ttagtgcaaa agagtgggag aaatatgcca
    28561 atatcaaatt tcagtttgta gcggataccg tccgcaatac aaatatcagg gttaagctgg
    28621 gaaaaggttc cgggcataat tctgctgttg gaaccgagtc taatttcaga tcgcagagtg
    28681 agcagactat gaatttcgat actttattct ttgcggatat tgattattat ctcgcaaaac
    28741 ttcaaagaaa gggtatcaag cctccgtata acctgaacca gctggctgca gaaatgaaag
    28801 aagagcccaa tcgctggaat gaaaaggaat tacgccgcgt tgttacacat gaattcggcc
    28861 atgcaatcgg attattgcat gagcaaagct ttccgggagc agttaactgg aaaagaacag
    28921 actccgttta taattattac ttacaaacgc agggatggga cagggctaaa gttgacttca
    28981 acgtatttga agtggctgac cagttttata ccaatggaac tacctatgat ccaaagtcga
    29041 tcatgcatta ctccattgaa ccatggcaaa caacagatgg atattcgctg aaagataatt
    29101 atgagctttc acagggtgac aaatcgttga ttgccgcatt atatccaaaa aatcaaaaag
    29161 tttccagcct ggtcgttcct aaagtggata ttacaaactt tgccaaactc aatgttgtat
    29221 caaatactac ccgcaacggg ctggttattg aaccttcatt tgatctgaag accaatccga
    29281 aattaggtga agtttatttt gtggcgaggc tggcaacaga agacggttat tatttaaaaa
    29341 caagtagcct ttattataac tggggcggaa ctgtggcagc ctataaaaag atgaatcttt
    29401 tacccaatac taaagtcagt tataataaaa caaagaagaa ttttgaattg tttttgccat
    29461 tcgactatgt gcctgaaacc tatggcaaaa aagtaattgt tgtgtttgct gtttacctgg
    29521 atgatgttgc aaataagcaa atggataaat tgatgtattt ttcaacaacc actccattaa
    29581 gcctgcccag ataaaattac catagaatga agtacaggcc tgcctttggc gggcctgttt
    29641 ttttcaggaa aaggggcccg attattcttt tcaaaaaacc acggtaaata ggtcagaagc
    29701 ctttatattt gcaccccaat tccgacttac ggattggagg cccgggaagt agcgcaggcc
    29761 ggtagcgcac ctggtttggg accagggggt cgcaggt
//
LOCUS       DAORTI010000030        29576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1119_length_29576_cov_9.563429, whole genome shotgun sequence.
ACCESSION   DAORTI010000030 DAORTI010000000
VERSION     DAORTI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 29576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29576
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1119_length_29576_cov_9.563429"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..708)
                     /locus_tag="PLO70_05910"
     CDS             complement(1..708)
                     /locus_tag="PLO70_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HQZ74028.1"
                     /translation="MKKLFPVFFVFAICCTLIVSCSKEPIDPIVRSFSNAGFTKIDAG
                     DQHRFTITSGTSFSIVARGEERDINDLLVQNAGDLLKIEYNRYKPNRKRVTFTITMPS
                     LEGVMLAGQSQATISGFSETVPVTLNMSGQAACWIHVNAPKFFAQASGQSKIEFLGGS
                     STGLETNASGQSEILAYGLMPVVTATAIATGQSTIKLKVGNSLNADASGQSRIYYQGN
                     PPIKNIIQTGSARIIQE"
     gene            complement(1148..1417)
                     /locus_tag="PLO70_05915"
     CDS             complement(1148..1417)
                     /locus_tag="PLO70_05915"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HQZ74029.1"
                     /translation="MKNKIIVLLLSVFSVATFGFAASVDVDPPLMKISFDKSFTSLEV
                     EDNIAVVLTNETGNEISLAGDKEYVKRVRTAVKRGRLYIYMAGRQ"
     gene            1630..2421
                     /locus_tag="PLO70_05920"
     CDS             1630..2421
                     /locus_tag="PLO70_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74030.1"
                     /translation="MAKQIIAACIIALLTSCSTAKMAVDESQWNNTQELAVKGRQGLL
                     IKQKLSFGEYKTVSVNRSWTKGSSSFAGWATGRPGYNDYERVIGTEYSQRKQTIRFEM
                     TDDKGNESSVFCLSRSREKDFMIGNNPNSLLNILLDISGKGDMSENTFWTKIFINNQS
                     RSWEMLLDNEASQRNSKSYTGVVAQSHDDYYTIHPVYKIAGKNEKVYTMPVGAVGFEL
                     RNKEGKPVAAVSAIDKGAVYLNSTDPGEKFLLSNICAAILLRESI"
     gene            2514..5555
                     /locus_tag="PLO70_05925"
     CDS             2514..5555
                     /locus_tag="PLO70_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ74031.1"
                     /translation="MRKLMLLAVLLNGLLAHAQQGYTFNRISTDDALGLSSNAVYCTY
                     QDKKGYIWVGTANGLQRFDGSKFVSFGNSDPGSTLLPISDLTQIIPGENGMFWLFFAS
                     RFEIGLFDPVSFRYNTVPVTPSKPIPARNNMKLWKDRHENLYLIIWKYGILRYNKVTH
                     SFSDENPFPLPNGWVSSIGVFEDTIKKQIWFPCPDSGLALYDLASKKMYSSSYNPLHI
                     PLLDNKKIIPGNTEIFIDSRRRHWVFNWTSAQHKWCFDERGNILKDTAGINDNREYSE
                     LANFFETKQQVLWVYGANALFNFDNDDKRFFFYKPGLTSETGIPYHSAHYIMEDRDGS
                     IWVSTDNGLFFATPESGTYGVVNILFDERKGGIDFTDLIQLRSGEYWLSTWGKGVITL
                     NKNFNTYKAGIYEHMPKMDPVSFSQYNQAWALYQHTDGKVWIGCQAGKYIVFDTITKK
                     SVFTELKEAEGATIRFITGDKNGNIWFSTQRGHIIFYDGKKFTVVQQFGTIIPKILID
                     NNRLLWLATFNQGLYCLSADGKTVLKHYTSDSKENALFINSGDDIDQINDTTIAYGAG
                     ALNLINTRTGKVTWRTFDEGLPGNSIRRIRADRNGNLWIITRNGLCKYNPRTNRFTPY
                     GRKDGITLANMTAAADFISDEDYILFAGSNGLMYFMPSLFENKKPPANVTITDFKLFN
                     DYLPLDSLLKRPKLEFNADENSFSIYFSSLSYSQKEKLTYYYKMDGVDKGWTKADRQN
                     FVNYPLLPPGHYTFSIYCENIDGIRSKEITSFEIYIKPPFWKTYWFVSTLLLIILLFI
                     YALHRLRVNKLLAVEKIRNRVARDLHDDMGSTLSTINILSTMAKSKLNSDNVKTSEFI
                     SKISENSQRMMEAMDDIVWSIKPMNDSMQKIIARMREYATNILEAKDIELLFNIEEEV
                     NDVKLDMEARRDFFLIFKEAVNNAAKYSKSEIVKISLLINNKQLTLEVIDDGEGFDVA
                     HADSGNGLSNMQKRADAMQGRLQITSKKGSGTTVKLAVPFK"
     gene            5565..6671
                     /locus_tag="PLO70_05930"
     CDS             5565..6671
                     /locus_tag="PLO70_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQZ74032.1"
                     /translation="MNKALSQKFRFFTFICIALLAYVHGYNLNNTYLAPYSTVEEPLT
                     VTSFFEYFIANGLLRFRIPMLFIISGYLYATYDHRPYLQQIQKRFKTLIIPFFLWSAA
                     GLVFTFLLQQFTYTSDVVYTAGIDQLGDNRPYTEIGWDGMLRRWLLHPVSYQLWFIVS
                     LFIYNLIYPVIRWLVVKIPFIWLPLTFLLFFSLFQFIYVDGRGIFFFSLGIWLQKKKI
                     SIDRQPEWFSAGLAFILFIGLTVIKTFMAFELEPNQFSTILTLVIFYQLSVITGIVAM
                     WFGLDKVVRWALRKKWFYKASGFSFFLYGAHVPLLPYAMTMALMELSFLPYYRLFCYI
                     VIPGLIVVLCIWIGRFIRKFLPGVYSTLTGSRGF"
     gene            6769..7401
                     /locus_tag="PLO70_05935"
     CDS             6769..7401
                     /locus_tag="PLO70_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74033.1"
                     /translation="MIKIMLYEDNPQLREGLTMLIDGSEGYQVLASFKNCNNVLSEVE
                     AFKPDVILMDIDMPGVNGIEGLKLIRQHNHEVKVLMLTVFDDNKNVFEALKSGANGYV
                     LKKTPPAKLLEYILEAASGGAPMTSSIATQVLKMFSEVQVPQGEDYNLSDREKEVLQH
                     LVNGYSYKMIASDMFIAIDTVRSHIKKIYEKLHVNSKSEAVAKAFKDKII"
     gene            complement(7470..8306)
                     /gene="tsf"
                     /locus_tag="PLO70_05940"
     CDS             complement(7470..8306)
                     /gene="tsf"
                     /locus_tag="PLO70_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQZ74034.1"
                     /translation="MSTVTISAQDINKLRQATGAGMMDCRKALTETNGDFEAAIDWLR
                     KQGQKVAAKRSDREAKEGVVIAQTTADNKTGIVVCVSCETDFVSKNADFVAFGKSIAD
                     AAIANNVTSLEQLMKVKIGDLTVAALVDEKLATIGEKIGVTKFQRVDAPYVASYIHGA
                     YRIGVLVGLNKEAAEPGKDVAMQIAALNPVAVDAASVPADVIAREKDIVLELMKQDPK
                     MAGKPEEMLAKIVDGKMGAFFKEQTLTAQAFVKDASKSVADYLKEAGDVQVTEFKRVA
                     LG"
     gene            complement(8429..9322)
                     /gene="rpsB"
                     /locus_tag="PLO70_05945"
     CDS             complement(8429..9322)
                     /gene="rpsB"
                     /locus_tag="PLO70_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQZ74035.1"
                     /translation="MENNTSLQQQLLEAGVHFGHLKKKWNPKMLPYIFAEKKGIHIID
                     LNKTVECLQETAAAMKQIARSGKKILFVGTKKQAKDIVNESAKRVNMPFVTERWLGGM
                     LTNFNTVRKSVKKMQSIEKMLGDGSFDSITKKERLTLSRDKDKMEKVLGGIAQLGRVP
                     AALFIVDIGHEHIALAEAKRLGISTFGVVDTNCDPNKVDFAIPANDDATKSIAIIVNY
                     ITAAIAEGLAERQAAKDEDVEEMTDDEKERKAAKLLAEAEAEGGRGGRSRGPGGAGGG
                     GQRRRSGSGTGGGRGPGGGRR"
     gene            complement(9428..9817)
                     /gene="rpsI"
                     /locus_tag="PLO70_05950"
     CDS             complement(9428..9817)
                     /gene="rpsI"
                     /locus_tag="PLO70_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQZ74036.1"
                     /translation="MEKQKNGIGRRKAAVTRVFLSKGEGSITVNDKDYKVYFPLVYLQ
                     NQVERPLKTVDVLNKFDIKINAAGGGVKGQAEAAMLGIARVLLEINPEFRPALKAAGL
                     LKRDPRSVERKKFGHKKARRSYQFSKR"
     gene            complement(9874..10317)
                     /gene="rplM"
                     /locus_tag="PLO70_05955"
     CDS             complement(9874..10317)
                     /gene="rplM"
                     /locus_tag="PLO70_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQZ74037.1"
                     /translation="MSKLHFTTKHANEATVKRNWYVVDGTNQTVGRMCAKIAAVLRGK
                     NKAYYTPHVDTGDYVIVINCEKIVLTGNKLDQKIYDHFTGYPGGRKEEVARNLINRRP
                     EVVIERAVKGMLPKNRLGRKMYKKLFVYVGAAHPHGAQQPKELKF"
     gene            complement(10540..11262)
                     /locus_tag="PLO70_05960"
     CDS             complement(10540..11262)
                     /locus_tag="PLO70_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74038.1"
                     /translation="MQTAMEEKILKGKISFVNYEKSFATIDYLSNTKEKSVNFKTSAP
                     DSGKKAHHFRLGDVVNFQLKLSARGDKMTACNIKFLYNPAIDLLIQKAAIENRFSGYL
                     KKVEEQYFVKEWDSYIFFPLEISPWEKPPVSTAENEAISFKLLNLDKPNAITAELFSH
                     NYIPEFKMAVQHFRNQVDTDAIVAKVSPYAVYLRLFNNTIQAKLPLEKGEVSELKEGD
                     VLQVKIKHLTNTRIVVEKVMAN"
     gene            11383..11985
                     /locus_tag="PLO70_05965"
     CDS             11383..11985
                     /locus_tag="PLO70_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQZ74039.1"
                     /translation="MSTRNLGRSTFGDPKNAGNLLTLEEAGILLNEWVPNEKLRLHMK
                     QVAANMKAWAIEKEGADEPTALKWELAGLLHDADWEKYPDDHCRVIVEELERRNIDPE
                     VIHCIASHGPSVFGVEPVSKMDKMIYAIDELSGFIHAAALIRPTLYEGLEVKSVLKRL
                     KTPSFAAQVSREDIQDAVSRIDIPLEELIQFIIDHQKKIS"
     gene            complement(12127..12324)
                     /locus_tag="PLO70_05970"
     CDS             complement(12127..12324)
                     /locus_tag="PLO70_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74040.1"
                     /translation="MLDIRIPIGLLFTVLGILLSAYGIYTNANTELYSKSFQFNVNLW
                     SGLGMLVFGVFMLLLVKKKKK"
     gene            complement(12343..14070)
                     /locus_tag="PLO70_05975"
     CDS             complement(12343..14070)
                     /locus_tag="PLO70_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQZ74041.1"
                     /translation="MGNALFHLEWIDYAIMIIYFIFVLGIGWVLKRYMKDANAFLEAG
                     RSMPAWVAGLAFISTNLGALEVMGMAGSGAKYGMLTTHFYWVGAIPAMVFLAIFMMPF
                     YYGSKARSVPEYLKLRFDEKTRGLNAILFAVMTILASGISMYALALLMEKVLGWDFNL
                     SLLLSAAIVLVYTYLGGLSSAIYNEVLQFFIIVIGLLPIVFLGLHEVGGWSGLKEALV
                     PIVSGKGFEGDSFTTTWKYTGQATSNPLGVEWYGILFGLGFVLSFGYWCTNFLIVQRA
                     MAAKSTTAARNTPLIGAIPKILIPFLIILPGIIALVLMNKTGSDFSINENGKPNYDLT
                     IIMLLKHYLPTGVLGLGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATGDKEA
                     DSKHYLKVGRMATVFGVLVSIAAAYLAGLFGNIMDFLQTIFSMINAPLFAVIFLGMFW
                     KRATGHGAFIGLLGGFLVALLHHGLTVPAGATTLVKGGWLGVMHTYPVEMAQNFWTAI
                     YACAASAIIAVVVSLSTTQKKTDDELRGLVYSLTPKIKEDHVVWYMRTNTLAIFVLTM
                     MVVLSVLFW"
     gene            complement(14127..15275)
                     /gene="galK"
                     /locus_tag="PLO70_05980"
     CDS             complement(14127..15275)
                     /gene="galK"
                     /locus_tag="PLO70_05980"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HQZ74042.1"
                     /translation="MGEYRVPEQLKKFVTEKTIIVRSPGRINLIGEHTDYNDGFVLPA
                     AIDKAVYVTIEQVPGTKLFLHSVDFNESVEIELTAIKPVPGHWSTYILGVADQFVQKA
                     KQVSGFKLNIYGDVPLGAGLSSSAAVECAVAFALNKILNAGFSRKELALMAQAAEHTY
                     AGVKCGIMDQFASLFGKKDNVIRLDCRSLDYEYFPLELNEYVVVLFDTQVKHSLASSE
                     YNVRREQCEAGVALIQQKYPQVKNLRDTTIEMIKECISPGDIVYNRCKYVVEEIIRLQ
                     TGCGDLQRGDLMAFGQKMYATHHGLSKYYEVSCKELDYLVEYVQQSRDVIGARMMGGG
                     FGGCTINIIKKSSVEGLVSELTVAYLENMGKELKHYMVNIEDGTSIIK"
     gene            complement(15286..18696)
                     /gene="galA"
                     /locus_tag="PLO70_05985"
     CDS             complement(15286..18696)
                     /gene="galA"
                     /locus_tag="PLO70_05985"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HQZ74043.1"
                     /translation="MKYTGFFLAIATGLSLVVSAQRQQLNFDNDWKFHFGHAANAEKD
                     FNYSIANIFSKSGAALKTAIDPKFDDGKWRKLNLPHDWAVELPFVNVDNFDVMAHGYK
                     PVGGLFPETSIGWYRKHFTVARSDSGQRFQLQFDGIFRDANIWLNGFYLGNNKSGYIG
                     AAYDITDYINFDKDNVLVVRADASQYEGWFYEGAGIYRHVWLNKYASVNIAKDGVFAY
                     SAIKGNISTITIETAVQNQYLDQANCTVYTFIKDRDGKKLAQSKELAVSLPVNGLSTT
                     KHSMTITGARLWSLEDPYLYRVVSVIKRNGKVVDSVKQRFGIRTIEIKPNGVFLNGQY
                     VKLKGVNNHQDHAGVGSALPDYLQYYRISLLKRMGVNAYRTSHNAPTPELLDACDSLG
                     MLVMDEQRLLNSSPEYIDQFERLIRRDRSRPSVFMWSIGNEEGWVHTNSTGKRIAQTL
                     IAKQKELDPTRTCTYAADLPNVFNGINEVIPVRSFNYRQFAVADYHRDHPDQPIIGTE
                     MGSTVTTRGVYEKDTVKGYLPDQDITAPWWASKAEDWWTLAATNDFWLGGFVWTGMDY
                     RGEPTPYQWPNINSHFGIMDMCGFPKNIYYYYKSWWTDKDVLHISPHWNWREKTAGWN
                     KKQGDPVEVWVNSNADNVELFLNGKSLGKKDMARNSHLKWTVNYEPGALEAVAYKKGK
                     RLTKKIETTGQPVEVVVTPYKTTILADGKDATVLNITVLDREGREVPDADNLIRFSIE
                     GDGKIIGVGNGDPSSHEPDKCADGAWQRTLFNGKCQVIIQSGTKPGMIKFDAKATGLW
                     SGGTDIQTISPESVATVTTDNKYKLTSEQAKRREVGKMLGADISFLPELEARGMKFSD
                     NGVEKDAIQILKDHGFNYVRLRIFNDPASDSGYSPGKGFCDLENTKKMAKRVKAAGMK
                     LLLDFHYSDYWADPGKQYKPAGWRKYSFEELKKAVYDYTKEVMQQLKEQGTTPDMVQV
                     GNEINHGIVWPEGNVSNFDGLAQLINAGTAAVKSVDPSVVMMLHVALGGQNDESVFFI
                     DNLVARGVHFDVIGESYYPKWHGTLEDLETNLDDLARRYNRDVIVVEYSHRKEEVNKI
                     AFEVAGGRGKGTCIWEPLSTWEKFFDDKGKANELLKLYDEISNKYLKN"
     gene            complement(18731..19774)
                     /locus_tag="PLO70_05990"
     CDS             complement(18731..19774)
                     /locus_tag="PLO70_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HQZ74044.1"
                     /translation="MKKIILYNKLRFIYTAFVLFIFVSCDPDKNDTPPPVVTPVQFAK
                     GADISWLTQMESSGRIFYNSSGTAQECMQLLKSLGMNSIRLRVWVDPAGGWNNTADVV
                     AKAIRAKNLGMKIMIDFHYSDSWADPGQQTKPAAWLAQDFTTLQTSVYNHTFNVLTAL
                     KSSGVTPEWVQVGNETNDGMLWPTGKASVNMANFAALINKGYDAVKAVDATIKVIVHI
                     SNGHDNSLFRWIFDGLKNNSGKWDIIGMSLYPDPANWSTMNSQCLANINDMIARYDKP
                     VMICEVGMSWDQAATCQSFLTDLINKVKSVPNNKGLGVFYWEPQCYGNWQGYTKGAFD
                     NSGKPTIALNAFN"
     gene            complement(19843..21606)
                     /locus_tag="PLO70_05995"
     CDS             complement(19843..21606)
                     /locus_tag="PLO70_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HQZ74045.1"
                     /translation="MKNIFKILTLAVTTVLMVTACTKVADLPYYDNGTAVVLTASKTS
                     VAPTPADSLSAVISFSWTSPEYKQDTTLYKFILEIDSTGRNFAKEATKIVTGVLTTSL
                     TGKELNTILLNYGFSLGVPYDLDVRVTSSYGNNNERYLSNVVKVSVTPYNDPAVLTTV
                     NTSVTCALATSTQLSNTFNWSNAFNGYTGNINYVLQYDSATKNFVAPLEIPVGTNLYT
                     KALTQGEMNETALTSGIPGGNTGKVEYRIKATTAQGAVSYSNVVNVTIQSYIPILRFY
                     LPGSYQAATGNGNNWDPGTAPEFIRDLRAGALNKLYYMYIYLPAGAEFKVTQGRSWDV
                     NYGGTAGVLSVGGANFTVATAGYYRISIDRTGLQYDIREGRMGFVGGGTGAGWDPPST
                     FPNYAMGAASTNLFVGLTDFTVDGWKLIDNNQWNSGDLTVVNTRSYGAAGGSGSTMEI
                     NGGNFPNVASAGRYRVMWDGRDVDNIKYEMSPATEMRVVGDGMQGVNAWDPGASPQMT
                     YNGNGVWSITLTLVAGKDIKFLAGNAWGAFDYEDNSGGSTATGTPRKIKWEGGDNFKT
                     PATTGSYTITLNEYTQTVTIN"
     gene            complement(21630..22457)
                     /locus_tag="PLO70_06000"
     CDS             complement(21630..22457)
                     /locus_tag="PLO70_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HQZ74046.1"
                     /translation="MKNMFKVLLGFLFLGFIISSCEKDENKIFFEGGTAPVLTASTTA
                     DQILLKVNENNPAIIFNWTNPDYKFTTGISSQDVTYTMQFDVAGANFGSAKKYEKAIA
                     RELTSTFTVRDLNAALLAMDLPENVVHNIEIRVKSSLVNNSVPLYSNVIAIKMTPYLD
                     VVYPVPANLYITGAATPGNWMGGGDPELLSQKFTKISTSEFEIASLAINPDAGFLFVP
                     VYGDWANKYGFTGAGLGNNVNGDSFQPGGNDFKSPPLAGNYKIVVSFKTGKYTITKL"
     gene            complement(22485..24092)
                     /locus_tag="PLO70_06005"
     CDS             complement(22485..24092)
                     /locus_tag="PLO70_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ74047.1"
                     /translation="MKHLSIVKILITGAVIVSLASCSKKLDLFPQNEFTSETAYANAD
                     GYRSVLAKVYGGLATTGNTGPAGSSDIQGLDEGSQSPFIRGFFNCQELPTDEAVVAWN
                     DQTIKDFHNLRWSSGDPFLKGMYARPIYNIMVANEYLRESTDDKLSSRGISGADADAI
                     KASRAEVRFLRAFNYWAMMDLFGKSTFIDETNLVGTSLPAEKSSQELFSFIESELLAI
                     ESDLKPVKTIEYGRVDQGACWALLARLYLNAQTYTGTSKYTEAITYASKVIGGGYALH
                     NNYAQLFLADNDKRTNEIIYAINCDGLRTSSYGNTTFFVHCAAGDDHNDYGVGGGWYG
                     YRATKGLADKFSDLTGATDKRALFTTSSFGTSPAQIVIDDISNFSNGLHVRKYRNIRS
                     DGAPTSDPNRDFSDVDFPVFRLSEMYLIYAEAVLRGGSGGSIANAVNYINLIRERAYS
                     NTSGNITSAQLTLPFILDERARELYWEGHRRTDLIRYGLLTTGTYLWPWKGGVASGTA
                     VDSKYNIFPIPASNITSNPNLTQNTGY"
     gene            complement(24112..27117)
                     /locus_tag="PLO70_06010"
     CDS             complement(24112..27117)
                     /locus_tag="PLO70_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ74048.1"
                     /translation="MSPQKLFKAAGLIVLALMAQISFAQDRVITGKVTDSKDGSPLQG
                     ATVAAKGSGSGAQTGSDGTFRISVPSTVNALIVSSVGFATKEVSIEGKNSIEVALVIT
                     NTSMDEVVVVSYGTRRKSDLTGSVTSVSAKDFQKGNINSPEQLLQGKVAGLQITTGGG
                     SAGGGSRIRIRGGASLNASNDPLIVIDGVPVEGNGIAGSANVLNSINPNDIESISVLK
                     DASATALYGSRASNGVLIVTTKKGVKGKLRFNYNNLFSVGTVGKTVEVLTAGQVTDII
                     NSDAAETGNNTYKNLLGSSNTNWQDEIYQSAFGIDNTISVSGGLGNVPFRASLGYLNQ
                     DGILKTNNFNRFSTALNLSPKLFENHLAVNLSVKASQTKNTFANEGAIGSSIAFDPTK
                     PVLSGNKEWGGYYEWLQANNLPIDLSTRNPLGLLNLRDNTSKVGRVIGNVDLDYKLHF
                     FPDLHIKMNFGLDYLSGSGNDNIDSVSATNYKTGGRKTFYEQKKVNTLADIFLFYEKN
                     IKSIDSKIDVLVGHSYQDFLTKVYNYPSFSYRAIADPNNPAKKDTIQGSEPTFPDDNP
                     RFRLESYLARVNFSIMNKYLITASIRSDASSKLNPNDRVGYFPAVAFAWKLKDEFFKN
                     ANVLNELKLRLGWGQTGNQDGIGYYSYLPRYSLGTNTAQYQFGNSFVSYLRPEGYDPN
                     LKWETTTTSNIGLDFGILRSRITGSVDYYYRKTEDLLADVDVPAGANFVNRITTNVGN
                     VISKGVEINLNTTPIKTENLTWDLGINYTYNEAEITNLLKNPDPNFKGQQVSGISGGT
                     GNFVGIHAVNYTPFTYLVYKQIYDDNNKPIEGLYEDVNRDGIINDDDRYFYKKPAADV
                     LLGLNTQVTYKDFSIGIAGHGSFGNYLYNNFFSNNGVLRSIKNPINFIGNASVDYLNT
                     RFVNNQYLSDYYIENASFFRLDNINLGYNVGRVFNDKASLRVAASIQNVFVISKYKGL
                     DPENAGDGGVDNNIYPRPRIFSLGFNFDF"
     gene            27698..28162
                     /locus_tag="PLO70_06015"
     CDS             27698..28162
                     /locus_tag="PLO70_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74049.1"
                     /translation="MEKKAGRSLEALLDKNVYYISGLTEIRLLAESPAQDQPAGYDSY
                     HDNIYTNTHIISNKNEETHIVVFKDILNNGGPQSKDFRYDMNSAKSTAGASRHGHPKQ
                     HSSFVVNILGEYDNQPVLLSHSQADYTITHSSGYAFTIPAFHGQTPHQPPDC"
     gene            28220..29545
                     /locus_tag="PLO70_06020"
     CDS             28220..29545
                     /locus_tag="PLO70_06020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HQZ74050.1"
                     /translation="MRLAIVAIAALLLAGACNKKNDYKQITSNPLLYSRTVKELNNVV
                     MQNNFPPIIASRNYLYANIAAYEIIAAGNSTRYSSLAGQVKHLTKIPAPPVGKEIDFN
                     FAAILAFCKLGEAVTFPEGSMKEYVDSLKTLVKEHGMPGSVYKNTVAYAEEVGKAILA
                     WSKKDNYAQTRTAPKYTVMDTPGRWIPTPPMYAQALEPNWREIRTMVMDSANQFTSPP
                     PPPFDISNKQSKYYQEVMAVKNAGDSLTDEQKHIAEFWDDLGTRLNVSGHVEFLTKKF
                     SPTGHWMNIVGIAAKTANVDFPTTVYAYAKTAIAMFDAFIQCWDEKFRSNTVRPETVI
                     NKYVDPDWRPYLQTPPFPEYTCGHSTVSASAAEALTSVFGDNFVYTDTSELEFGIKNR
                     SFTSFRQAAEENNWARFYGGIHFHNSCIISTEYGKKVGDLVNQRLQMKK"
BASE COUNT         8797 a         6421 c         5498 g         8860 t
ORIGIN      
        1 ttattcctgg attatcctgg cagacccggt ttgaattata ttttttattg gcggattacc
       61 ctgatagtat atcctgcttt gaccagaagc gtctgcgttg agtgaattgc caacttttag
      121 cttaattgtt gactgccccg ttgcaatcgc agtggctgtt actacaggca tcaggccata
      181 ggcaagaatt tcagattgac cgctggcatt agtttccagc ccagtgctac taccacccag
      241 gaattcaatt tttgactggc cgctggcctg tgcaaagaat tttggtgcat ttacatgtat
      301 ccaacaagct gcctggccgg acatattcag cgttacagga acagtttcag aaaaccctga
      361 aatagtagcc tgagactgac cggccagcat tacaccttca agagaaggca tagtaatagt
      421 aaaagtgacc cttttgcgat tgggtttata acggttatat tcaatcttca acagatcacc
      481 ggcattctgt acaagaagat catttatatc cctttcttca ccacgggcca caatagaaaa
      541 actggttccg cttgtaattg taaaacgatg ctgatcaccg gcatcaattt tcgtaaaacc
      601 agcattactg aaactacgaa cgattgggtc aattggctct tttgagcagg aaacgatcaa
      661 agtacagcaa atagcgaaca caaaaaatac aggaaacaat tttttcatct gaataaggtt
      721 taaggttatt gataagaatt acacaaaaat gttataatca actggtaaaa gcaatcgcat
      781 gaatatgtga aaaagaaaag ccctctttag acaaagaagg cctgttatga ttgcttatat
      841 aggagagagt tatttaagtt ttcttactat ctcaaggtaa cgttagtaca gagttaacaa
      901 tcctgttccc tttattagga tcattgttgg cggaaatatt atttatgcac ctctaaattc
      961 atactcactt gtaccactaa tatcgatctt accccttgac ttgataaaaa gcttgcaggc
     1021 accattgacc acaaccagca ttcctgcatt atgtaaggct gatgctgtgc tgacatatga
     1081 atctccgttt ataacaactt gtcagagtaa agctgcagga acataaacag tcaccctttc
     1141 ctctttttca ctgccggcca gccatatata tatataaccg acctcgcttt actgcagttc
     1201 ttactctctt tacgtattcc ttatcacccg ccagcgaaat ttcattcccg gtttcattgg
     1261 tcaataccac agcaatatta tcttctactt caagactggt aaatgattta tcaaaagaaa
     1321 tcttcatcaa tggcggatcg acatcaacag aagctgcgaa accgaatgta gccacggaga
     1381 atactgataa tagcaataca ataattttgt ttttcatgat tttcaatttg taaggtttat
     1441 ttattttaat gacagatcaa atgtcagtat ataaatcagg caaaaaaata acataagtat
     1501 gtacaattaa tgaccttaga cctcttccgg ctcacataaa tatgttaacc ggggtatctt
     1561 ttctgatttc acatattcat gtgatataaa atatttagga ggtcaaataa ttttgtctga
     1621 aattaaatta tggcaaagca aataatagct gcatgtatta ttgcactact gacatcctgc
     1681 tcaactgcaa agatggcggt ggatgaatct caatggaata acacacaaga actggcggta
     1741 aaagggcgac agggcttact gatcaaacaa aaactcagtt ttggtgagta taaaacagtt
     1801 tctgtaaacc gttcctggac aaaaggcagt agtagttttg caggctgggc taccggcaga
     1861 cccggctata atgattacga aagagtgatc ggcactgagt attcacagcg caaacaaact
     1921 atccgctttg agatgactga tgataaaggc aatgaaagca gtgttttttg cttatcccgg
     1981 tcaagggaga aggattttat gatcggcaat aaccccaaca gcctgcttaa tatattgctg
     2041 gacatctctg gtaaaggcga tatgtctgaa aatacattct ggaccaaaat atttatcaat
     2101 aaccaatcca gatcatggga gatgttactt gataatgaag cttcacaaag aaatagtaaa
     2161 tcctatactg gagtagtggc tcaaagccac gatgattatt atacaattca ccctgtatat
     2221 aaaatagcag gtaaaaatga gaaggtatat acaatgcctg taggcgctgt ggggtttgag
     2281 ttaagaaaca aagaaggaaa accggttgct gctgtatcag ctattgataa gggggcagtt
     2341 tacttaaaca gtactgatcc gggagaaaaa ttcctgcttt ccaatatctg tgcagctata
     2401 cttctcaggg aatctattta agataaaggg acaaggagcc aatccccaca taaatatgtg
     2461 gtttttaaaa agcgaccttt catatgccgg ttccacttgt tagatttgta tatatgagaa
     2521 aactgatgct tttggctgta ttgctgaatg gccttttggc acatgcccag caaggctata
     2581 cattcaatcg tatcagtact gatgatgcac ttggtctttc ttccaacgct gtttattgca
     2641 cttaccagga taaaaagggt tatatatggg taggcaccgc caatggatta cagcgctttg
     2701 atggcagcaa gtttgtgagc tttggtaaca gcgatcctgg tagtacatta ttacctattt
     2761 ctgatcttac ccagataatt cccggtgaaa atggtatgtt ctggctgttt tttgcatccc
     2821 gctttgaaat agggctgttt gaccccgttt cattccgtta taatactgta cctgttacac
     2881 catctaaacc catacctgcc aggaacaaca tgaagctctg gaaagataga catgagaatt
     2941 tatatcttat tatatggaaa tatggtattc ttcgttataa caaggtcaca catagctttt
     3001 cagacgaaaa tccttttcca cttccaaatg gctgggtatc ttctattggt gtatttgaag
     3061 acaccataaa aaagcagatc tggtttccct gccctgacag cggtcttgct ttatacgatc
     3121 ttgcctcaaa gaaaatgtat tccagcagct ataatccctt acatattccg ctacttgata
     3181 ataagaagat aattccgggg aacactgaaa tatttattga tagccgcaga aggcactggg
     3241 tattcaactg gaccagtgcc cagcataaat ggtgttttga tgaaagagga aatattttga
     3301 aagatacagc cgggatcaat gacaaccgag aatattctga acttgccaat ttttttgaaa
     3361 caaaacaaca ggtattatgg gtatatggag caaatgcact tttcaatttt gataatgatg
     3421 ataaaagatt tttcttttat aagcctggct taacaagtga gacaggtatt ccttatcatt
     3481 ctgcacatta tattatggaa gacagggatg gcagtatctg ggtatctaca gataacggac
     3541 ttttttttgc tactcctgaa agcggcactt atggtgtggt caacatactg tttgatgaaa
     3601 gaaagggggg cattgatttt actgacctta tacagttgag aagcggcgaa tactggctaa
     3661 gtacctgggg gaagggagta ataacactga ataaaaattt taatacctat aaggcaggca
     3721 tttatgagca tatgcctaaa atggacccgg taagtttttc acaatacaac caggcttggg
     3781 cattatatca gcatacagat ggaaaggttt ggattggttg tcaggctggt aaatacattg
     3841 tctttgacac tattacaaaa aaatcagttt tcactgaact aaaagaagcg gaaggggcca
     3901 ccattcgctt tattaccgga gataaaaatg gcaatatttg gttttctaca caaagagggc
     3961 acatcatttt ttatgatgga aaaaaattta ctgttgttca gcaattcgga accataattc
     4021 ccaaaatatt aatagacaat aacaggttgc tttggctggc aacttttaac caggggcttt
     4081 actgcctgag tgctgatggt aaaacagttc taaaacacta taccagcgac agtaaagaaa
     4141 atgcactgtt cattaactct ggtgacgaca tagatcaaat caatgacaca acgattgcgt
     4201 atggtgcagg cgccctgaat cttatcaata caagaaccgg aaaagtaacc tggcgcacat
     4261 ttgatgaagg attgccgggc aattcaatca ggcgtatacg ggcagacaga aatggcaact
     4321 tgtggatcat tacaaggaac ggattgtgta aatataatcc ccggaccaac cgctttactc
     4381 cttatggcag aaaagatggc attacgctgg ccaatatgac tgcagcagca gattttatca
     4441 gtgatgaaga ctatattcta tttgccggat caaatgggct aatgtatttt atgccatctc
     4501 tttttgaaaa taaaaagccg ccggctaatg taactattac tgattttaaa ttattcaacg
     4561 actatcttcc attagattct ttacttaagc ggccaaaact agagtttaac gctgacgaaa
     4621 attcttttag tatctacttc tcttcactca gctattctca aaaggagaaa ctgacctact
     4681 attataaaat ggacggagta gacaaggggt ggacaaaagc agaccggcag aactttgtga
     4741 actatccgct gctgcctccc ggccattata cctttagtat ttactgcgaa aacattgatg
     4801 gtatccgctc taaggagatt acctcatttg aaatctatat taaacctcct ttctggaaaa
     4861 cctattggtt tgtcagcacc ttattactga ttatcctgtt gtttatctat gcattacacc
     4921 ggcttcgggt taataagtta ctggccgttg aaaagatcag gaaccgggtg gccagggatc
     4981 tgcatgacga tatgggatct actcttagca ccattaatat actcagtaca atggcaaaaa
     5041 gtaaactgaa ttctgataat gtaaaaacaa gtgagtttat cagcaagatc agcgagaaca
     5101 gccagcgtat gatggaagca atggatgaca ttgtctggag tataaagccc atgaacgaca
     5161 gtatgcagaa gatcattgcg aggatgaggg agtatgccac caatatactt gaggcaaaag
     5221 acattgaatt gttgtttaac atagaagaag aagtaaatga tgtaaaactg gatatggagg
     5281 cccgcaggga cttcttcctc atattcaaag aagcagtgaa caatgcagcg aagtattcaa
     5341 aatccgaaat agtaaagatc agcctgctta ttaataataa acagcttacc cttgaagtca
     5401 ttgatgatgg agaaggtttt gatgtggctc atgctgatag tggaaacgga cttagcaata
     5461 tgcaaaaaag agctgatgct atgcagggaa gattgcagat tacttcaaaa aaaggaagtg
     5521 gtactactgt aaaacttgct gtgccattca aataaacaat aactatgaat aaagcactaa
     5581 gccagaaatt tcggttcttc acattcatct gcattgcact gctggcttat gtgcatggtt
     5641 ataacctgaa taatacctac ctggcgcctt atagcacagt agaagaacca ttgactgtta
     5701 cctcattttt tgaatacttt atagcaaacg gcctgctcag gttcaggata ccaatgctct
     5761 ttattatctc tggctacttg tatgctacct atgatcaccg gccttacctg cagcaaatac
     5821 aaaaaagatt caaaacgtta atcattccct tttttctatg gagtgcagca ggtcttgtct
     5881 ttacttttct attacaacaa tttacctata cttccgatgt agtttatacg gcaggcatag
     5941 accagcttgg ggataacagg ccttatactg aaattggctg ggacgggatg cttagaaggt
     6001 ggctgctgca tcctgtgtca tatcagttat ggtttattgt ttcacttttt atttataatc
     6061 tcatttatcc ggttatcagg tggctggtgg taaaaattcc ttttatatgg ttgccgttga
     6121 cttttctgct tttcttttct ctgttccagt ttatttatgt tgatggcagg ggcattttct
     6181 ttttctcatt gggaatttgg cttcaaaaaa agaaaatatc tattgataga caaccggaat
     6241 ggttcagtgc cggattagct ttcatacttt tcataggatt gacggtaatt aaaacattta
     6301 tggctttcga actggagccc aaccagtttt caaccatcct taccctggta attttttatc
     6361 aattatctgt tattaccgga atagtagcaa tgtggtttgg cctggataaa gtggttcgct
     6421 gggcactaag aaagaagtgg ttctacaaag ccagtggctt ctcctttttt ctttacggcg
     6481 ctcatgtgcc tcttttacca tatgccatga ccatggcact aatggaactg agctttttac
     6541 catattacag gctattctgc tacattgtga tacccgggct tattgtcgta ctttgtatct
     6601 ggataggccg atttatccgc aaattcttac caggcgtcta ttctaccctt accggaagca
     6661 gggggttctg attatcgggc tattacacat taatatgtga taggaagcca gtaagagggg
     6721 tggtaaattt gcatgaatgg ctataattca tcctttcaac tttatactat gataaagatt
     6781 atgctgtacg aagacaaccc gcaactgaga gaaggtttaa cgatgctgat tgatggcagc
     6841 gaaggttatc aggttttagc atcttttaaa aactgtaata atgtattgtc agaagtagag
     6901 gccttcaaac cagatgttat tctgatggat atcgatatgc cgggagtaaa tggaattgaa
     6961 ggattaaaat taatccgaca gcacaatcat gaggttaaag tactgatgct aacagtcttt
     7021 gatgataata aaaatgtttt tgaagcatta aaaagtggtg ctaatggtta tgtattgaaa
     7081 aaaactcctc cggcaaagct gcttgaatat attttagagg ctgccagtgg cggagccccg
     7141 atgacatcct ccatcgccac acaggtatta aaaatgtttt ccgaagtaca ggtgccccag
     7201 ggagaagatt ataatcttag cgacagggaa aaagaagtgt tgcaacatct tgttaatggc
     7261 tatagttaca aaatgatcgc ttcggatatg ttcattgcga ttgatacagt acgcagtcat
     7321 attaaaaaga tttatgaaaa actgcatgta aattctaaat ccgaggcggt agcaaaagca
     7381 tttaaggata aaataatata gtcatgagtc taataataaa agggggaatc aaatgattcc
     7441 cccttttatt atttagttag ttataagaat tatcccagtg ccactctttt aaactccgta
     7501 acctgtacat ctcccgcttc tttcaggtaa tcagcaacag acttacttgc atcttttaca
     7561 aaagcctggg ctgttaatgt ttgctcttta aaaaaggcac ccattttgcc atctacaatt
     7621 ttagcaagca tttcttctgg ttttccggcc attttgggat cctgcttcat caattccagc
     7681 acaatgtctt tctcacgggc aattacatct gcaggcacac tggctgcatc taccgcaaca
     7741 gggttcaggg cagcaatttg catggctacg tctttacccg gctcagcagc ttctttattc
     7801 aatcctacta atacaccaat ccggtatgct ccgtgaatat aagaagccac atatggtgca
     7861 tctactcttt ggaattttgt cacaccgatc ttctcgccga tagttgccag cttttcatca
     7921 accagggcag caactgtaag atcgccgatt tttactttca tcaactgctc aaggctggta
     7981 acattattag caattgcagc atcagcaata cttttaccaa aagcaacaaa atctgcattc
     8041 ttggaaacaa aatcagtttc gcagctcaca caaacaacaa ttcctgtttt gttatcagca
     8101 gttgtttggg caatcaccac tccttctttt gcttcacggt cacttctttt ggcagccact
     8161 ttctgaccct gcttgcgtaa ccagtcaatc gcagcttcaa aatcaccatt ggtttcagtc
     8221 aacgctttac ggcaatccat catgccggca ccagtagcct ggcgaagctt attaatatct
     8281 tgtgcactta tagttacagt actcataatt ataaattgaa gtctgaagta agaagtctga
     8341 taggactccg ctcttcaggg tttaaaaatt tattgaatga attttctttt ttgtcagatt
     8401 tcatagttct gacttcccgc ttcagctttt atcttcttcc accaccaggt ccacgaccac
     8461 cacccgttcc actaccagaa cggcgtcttt gcccgccacc accagcacca ccgggaccac
     8521 gacttcttcc gccacgacca ccttcagctt ctgcctctgc cagtaatttt gctgcttttc
     8581 tttccttttc atcgtcagtc atctcttcta catcctcatc cttagcagct tgtctttcgg
     8641 ccaaaccttc tgcgatagca gcggtgatat aattaacgat aatagcaatt gatttggtag
     8701 catcatcatt cgccggaata gcaaaatcaa ccttattagg atcacagtta gtgtctacaa
     8761 caccaaatgt agaaatacct aaacgctttg cttctgccaa tgcgatatgc tcatgtccta
     8821 tatcaacaat aaacaaagca gcaggtacac ggcccagttg ggcaatacca cccaatactt
     8881 tctccatttt atctttatca cggctaagtg tcagacgttc tttcttggta atactgtcaa
     8941 aagaaccatc acccagcatt ttctcaatgc tctgcatttt cttcacactc ttacggacag
     9001 tgttgaagtt tgtcagcatt ccacccaacc atctttcggt aacgaatggc atgtttaccc
     9061 tctttgcact ttcgtttacg atgtctttgg cttgtttctt agtaccaacg aacaaaattt
     9121 tcttaccgct gcgggcaatc tgcttcatgg cagctgcagt ttcctgcaaa cattcaacag
     9181 ttttgttcag gtcaatgatg tggatacctt tcttctcagc aaagatgtaa ggcaacatct
     9241 tggggttcca cttctttttc aggtgaccaa aatgaacgcc tgcttccaga agttgctgtt
     9301 gtaaggatgt attattttcc attttttatt ttaagctttt agctattagc tgtttgccgt
     9361 tagccagtta aaaattaaat tttgttttcc aagctgattg ccaaaggcca ttagctcagc
     9421 gcttctatta acgtttagag aactggtaac tacgacgggc tttcttatga ccgaatttct
     9481 tacgttcaac agatctcgga tcccttttca acaaacctgc agcttttaat gcaggacgaa
     9541 actcaggatt gatttcgagt aataccctgg cgatacccag catagctgct tctgcctgtc
     9601 cttttacacc accaccggca gcgttaattt ttatatcaaa cttattaagt acatctactg
     9661 ttttcagcgg acgctccacc tggttttgca agtataccag gggaaaatat actttatagt
     9721 ctttatcgtt tactgtaatg ctgccttctc ctttggagag gaacactctt gttacagcgg
     9781 ctttacgacg accaattccg tttttttgct tttccatttt attttatttg gaatatttaa
     9841 agtttgtttt atcaacccga gccaaagcga gggttaaaat tttaattctt taggttgttg
     9901 agctccatgt ggatgtgctg cacctacgta cacgaataat ttcttgtaca tcttacggcc
     9961 caggcgattt ttaggaagca tgccttttac agctctttca ataaccactt ccggtctgcg
    10021 gttgatcagg ttcctggcca cttcttcttt acgacctccg ggatacccgg taaagtggtc
    10081 atagattttc tggtccagtt tattacctgt taaaacaatt ttttcgcagt tgatcacaat
    10141 aacataatct cctgtatcaa cgtgaggggt gtagtatgcc ttattcttac cacggagtac
    10201 cgcagcaatt ttagcacaca tacgaccaac ggtttgatta gtaccgtcca caacgtacca
    10261 gttgcgtttt acggtagcct cgttcgcatg tttggtagtg aaatgtaatt tgctcatttt
    10321 aatttctgtt taaaggccgc cgctatcccg gcaaccggtt ttttaaatgg atggcaaagg
    10381 tagggctgag tcaaacgcaa acctaagatt tgagaaagta attttgatcg aagcctcata
    10441 aataattgat tatcaataaa atttttgacc aattatttaa gtatcccaga tcgggggctg
    10501 atagatcctc tattttcaga actaactaag agagaattat taattggcca ttaccttttc
    10561 aacaacgatt cgggtattgg tcaggtgctt aatctttacc tgcagcacat ctccttcctt
    10621 taattcagat acttcccctt tttccaatgg aagctttgcc tgtattgtat tattaaataa
    10681 ccggagatag accgcatagg gagacacctt agctacaatt gcatctgtat cgacctggtt
    10741 cctgaaatgt tgtaccgcca ttttaaattc aggaatgtag ttatgagaaa acaattctgc
    10801 tgtaatggca ttgggtttat caagattcaa cagtttaaaa ctaatcgctt cattttccgc
    10861 agtagatacg ggcggctttt cccagggtga tatctccagg ggaaagaaga tataactatc
    10921 ccattctttt acaaaatact gctcctctac ctttttaaga taaccggaaa aacggttctc
    10981 aatagctgct ttctgaatta agaggtctat agccgggtta tacagaaatt taatattaca
    11041 ggctgtcatt ttatcgccac gggctgataa tttcagctga aaatttacaa catcacccaa
    11101 ccggaaatga tgtgcttttt tcccgctatc cggggcactt gttttaaaat tgactgattt
    11161 ttctttggta ttacttaaat agtcaattgt agcaaaagat ttttcataat taacaaatga
    11221 aatcttccct ttgagtattt tttcttccat tgctgtttgc atctatcgca aaggtagatg
    11281 agctcccgaa tatgatacca gtaaatttga aacggcattt tattcaaaac gggtttattt
    11341 aatttcaaaa tccttttata tttagaatca aattcagcat acatgtcaac cagaaatctt
    11401 ggccgttcca cttttggtga ccccaaaaat gcggggaacc tgctaacact tgaagaagca
    11461 ggcattctat taaatgagtg ggtgcccaat gaaaagctcc ggctgcatat gaaacaggta
    11521 gctgcgaaca tgaaagcatg ggccatcgaa aaagaagggg cagatgaacc aactgccctg
    11581 aaatgggaat tggccggctt gctgcatgat gctgactggg aaaaatatcc tgacgatcac
    11641 tgccgggtga ttgtggaaga actggaaaga agaaatattg acccggaagt aattcattgt
    11701 atcgcttcac acggaccatc tgtttttggc gttgagccag tcagtaagat ggacaaaatg
    11761 atctatgcca ttgatgaatt gtctggtttt attcatgcag ctgctttaat caggccgaca
    11821 ttatatgagg gcctggaagt gaaaagtgtg ctgaaaagat taaaaactcc atcattcgct
    11881 gcccaggtaa gcagggagga tatacaggat gccgtatccc gaattgatat tccattggaa
    11941 gaattgatac aatttattat tgatcatcag aaaaaaatta gctgattgaa ataacaattt
    12001 cagtgttatt tcaatcagct aaacgaaagg ttttctaaat caagtttacc cggggagcaa
    12061 taaacaaagc cgctaactat tcccgacacg actgcaactg aagcgaatgc acctcaaacc
    12121 gggaacttat tttttcttct tctttaccaa caacagcata aataccccaa ataccagcat
    12181 gcctaatccc gaccagaggt ttacattgaa ttgaaaagat ttggaataaa gttctgtgtt
    12241 ggcattggta tatataccgt aggcagaaag taatatgcct aaaacagtaa ataacaaccc
    12301 gattggaatt cttatatcta acataatttt ttcgtttaat ttttaccaga ataaaacaga
    12361 taatacaacc atcatggtta aaacaaatat ggcaagggta ttggtacgca tataccacac
    12421 cacatgatcc tcttttattt ttggagtcag ggaataaacc aatccacgca gctcatcatc
    12481 ggttttcttt tgtgtggttg ataaagacac aaccacagca atgatagccg aagctgcaca
    12541 tgcataaata gctgtccaga aattctgtgc catctcaacc gggtatgtat gcatcacgcc
    12601 taaccaaccc cctttgacca atgtggtagc cccggccgga actgtgaggc catgatgcag
    12661 caaagcaaca aggaaaccgc ccagcaaacc tatgaaagca ccgtgcccgg tagctctttt
    12721 ccagaacatg ccgaggaaaa tcactgcaaa caaaggagca ttgatcatgg agaaaattgt
    12781 ttgtaaaaag tccatgatat tgccaaataa cccggccagg taagcagcag caatactgac
    12841 taaaacacca aatacagtgg ccattctacc cactttaaga tagtgttttg aatcagcttc
    12901 tttgtcacct gtagcaggcc tgatatagct ttgataaata tcataggtcc atactgtatt
    12961 gaatgcagaa acattacccg ccattccaga catgaaagat gctaacaatg ccgttatgcc
    13021 aaggcctaat acacctgttg gcaaatagtg ctttaataac ataatgatgg taagatcata
    13081 attcggctta ccattttcat taattgaaaa atctgaaccg gttttattca ttagtacgag
    13141 ggcaataatc cccggaagaa taataagaaa aggaatcagg attttgggaa tagcaccgat
    13201 caatggtgta ttacgggctg ctgttgtaga ttttgcagcc attgcccgtt gtacgatcag
    13261 gaaattggta caccaataac caaaagaaag aacaaatccc aagccgaaca gaattccata
    13321 ccactccacc ccaagaggat ttgacgttgc ctggcctgta tatttccagg tagtggtaaa
    13381 actgtctcct tcaaatccct ttccggaaac tatcgggaca agtgcttctt ttaatccgct
    13441 ccaaccccct acttcatgaa gacctaagaa aacaattggc aataaaccaa tgacaattat
    13501 aaaaaactgc agcacttcgt tataaatagc agatgacagc cctccaagat aggtatacac
    13561 caatacgatg gcagcggata ataaaagact aagattaaaa tcccatccca acactttttc
    13621 catcaataag gcaagggcat acattgaaat tcctgatgcg agtattgtca tgactgcaaa
    13681 cagaatagca tttagaccac gggtcttttc atcgaaacgt aatttcaaat attcgggaac
    13741 agaacgggct tttgaaccat aataaaatgg catcataaaa atggccagga aaaccattgc
    13801 aggtattgct ccgacccagt aaaaatgggt ggtgagcata ccatacttgg cgcctgagcc
    13861 cgccataccc attacttcca gggcgccaag attggttgaa ataaaagcca accctgccac
    13921 ccaggcaggc atagatcttc ctgcctccaa aaaggcatta gcatccttca tgtatctttt
    13981 caaaacccat ccaatcccca gcacaaatat gaaatatatg atcattatgg cataatcgat
    14041 ccactccagg tgaaataaag catttcccat ttgtattgtt ttaaattatc aagaagttga
    14101 ttttacagta aggagaaact gatccgtcat tttattatag atgttccgtc ttcaatattg
    14161 accatataat gtttcagctc tttacccata ttttctaagt aggccacagt tagctcacta
    14221 accaaccctt ccacagaaga tttcttaata atattgatag tacacccgcc aaatccgcca
    14281 cccatcatcc ttgcaccgat cacatcccgg ctttgttgta catactcaac aagataatcc
    14341 aattctttac aacttacttc gtagtatttg cttaatccat gatgtgtagc atacattttt
    14401 tgtccgaagg ccatcaggtc gcctctttgt aaatccccgc atcctgtttg cagacggatg
    14461 atctcctcaa ctacatactt acaacggtta tacacaatat ctcccggact gatacattct
    14521 ttgatcattt caatagttgt atcccgcaaa ttttttacct gaggatactt ttgttgtatc
    14581 aatgcaacac ctgcctcaca ctgttcccgg cggacattat actcagaaga ggccagcgaa
    14641 tgctttacct gtgtatcaaa aagcacaact acatactcat ttaactccag cggaaaatat
    14701 tcatagtcaa gtgaacgaca atccaacctg atcacattat cctttttacc aaaaagactg
    14761 gcaaactggt ccataatacc acacttcaca cctgcatagg tatgttctgc cgcctgcgcc
    14821 attaatgcta actcctttct tgaaaatccg gcattcaata ttttatttaa tgcaaaagca
    14881 acagcacatt ctactgcggc cgatgaagaa agacctgcgc ccaacggcac atcgccataa
    14941 atattcaact taaagccact tacctgtttc gccttctgta cgaactgatc tgccacgccc
    15001 aaaatatatg ttgaccaatg accaggtaca ggtttaatag ctgttaattc gatttcaaca
    15061 gactcgttga aatcaactga atgtaaaaat aatttcgttc ccggcacctg ttcaatggtt
    15121 acataaaccg ctttatcaat ggcagccggc aatacaaatc catcattata atcagtgtgt
    15181 tcaccaatca ggttgatacg gcccggcgat cttacaatga ttgttttttc ggtgacgaat
    15241 ttttttagct gctccggaac cctgtattct cccactttta tgtttttagt tttttaaata
    15301 tttattgctt atctcatcat ataacttcag cagttcattg gctttccctt tatcatcaaa
    15361 gaatttttcc caggtactca gcggttccca gatacaagtg cccttccctc ttccgccggc
    15421 cacttcaaaa gcgatcttat ttacttcttc tttgcgatga gaatattcca ccacgatcac
    15481 atcacggtta taccttctgg caaggtcatc caggttagtt tccaggtctt ccagtgtgcc
    15541 atgccatttg ggataatacg attcgccgat cacatcaaaa tgcacacccc gggccaccaa
    15601 attgtcaatg aaaaaaactg attcatcatt ttgtccgccg agtgccacat gcagcatcat
    15661 gacaacagac gggtcaactg attttacagc tgcagtacct gcattgatca gttgtgccaa
    15721 tccatcaaaa ttgctgacat tgccttccgg ccaaacaata ccatggttga tctcattgcc
    15781 aacctgtacc atatccggag tagtaccctg ttctttgagt tgctgcatca cctctttggt
    15841 atagtcatac acagctttct tcaattcttc aaatgaatat tttctccatc ctgcaggttt
    15901 atactgcttg cccggatcag cccaataatc gctgtaatga aaatctaaca ggagtttcat
    15961 acctgctgct tttactctct tcgccatttt ctttgtattc tccagatcac aaaaaccttt
    16021 accgggagaa taaccactat cgcttgcagg atcattaaaa atgcggagtc gtacataatt
    16081 aaatccatgg tctttcagaa tctgtatcgc atctttttct acgccattgt cagaaaactt
    16141 cattcctctt gcttccagtt cgggcaggaa agaaatatca gcacccagca tttttcccac
    16201 ttctcttcgt tttgcctgct cgctggttag tttatattta ttgtctgttg taactgttgc
    16261 cacactttcc ggagatatcg tttgtatatc tgttcctcca gaccaaagac cagttgcttt
    16321 agcatcaaat tttatcatgc cgggttttgt accagactga ataattacct gacacttgcc
    16381 attgaacaaa gtccgctgcc aggcaccatc tgcacattta tctggttcgt ggctgctggg
    16441 atctccattg ccaacgccta ttattttccc atccccttca atactgaacc tgatcaggtt
    16501 atccgcatct ggtacctcac ggccttccct gtcaagtaca gtgatgttta gcacagtggc
    16561 atctttccca tcagccaata ttgttgtttt ataaggtgta accactactt caaccggctg
    16621 acccgtagtt tctattttct tagtgagtct tttccccttt ttatacgcca ccgcttctaa
    16681 ggccccgggt tcataattca ccgtccattt caaatgacta ttgcgggcca tatctttttt
    16741 gcctaaactt ttcccattca ggaataactc tacattatct gcattgctgt ttacccatac
    16801 ttccacagga tcgccttgct ttttattcca gcccgccgtt ttctctctcc agttccagtg
    16861 cggtgaaata tgcaacacat ctttatccgt ccaccaactc ttatagtagt aatatatgtt
    16921 tttggggaaa ccgcacatat ccataatacc aaaatgagaa ttgatatttg gccattgata
    16981 aggtgtaggc tcaccacgat aatccatacc cgtccataca aatcctccca gccagaaatc
    17041 attagtagct gctaatgtcc accagtcttc tgctttgctg gcccaccagg gtgccgtaat
    17101 atcctggtcc ggcagatagc ctttaactgt atctttttca taaacacctc ttgttgttac
    17161 cgtacttccc atctcggtac caatgatagg ctgatcggga tgatcacgat ggtaatcagc
    17221 tactgcaaac tgccggtaat taaatgaacg gacaggaatc acttcgttga ttccattgaa
    17281 tacattggga agatcagctg cgtaggtgca tgttcttgtt ggatctaatt ctttttgttt
    17341 ggcaatcagg gtttgtgcta ttcttttacc ggtgctgttg gtgtgcaccc aaccttcttc
    17401 attgccgata gaccacataa aaacagatgg cctgctgcgg tctcttctta tgagccgttc
    17461 aaactgatca atatactcgg gactgctatt cagtaatcgc tgttcatcca tcaccagcat
    17521 acccaaacta tcgcaggcat cgagaagttc tggtgttggt gcgttatgac tggtacgata
    17581 tgcattaaca cccattcgct tcagcaagct gatccggtaa tattgaaggt aatccggcaa
    17641 ggcactgcct actcctgcat gatcctggtg attgtttaca cctttcagtt ttacatattg
    17701 accattcagg aaaactccgt tgggttttat ttcaatagtg cggatgccaa aacgctgctt
    17761 tacactatca accactttcc cgtttcgttt gatcactgaa acaactctgt acaagtatgg
    17821 gtcttccagc gaccaaagcc ttgccccggt aatagtcata ctatgttttg ttgttgacaa
    17881 accattgact ggtaatgaaa cggccagttc ctttgattgc gccagcttct ttccatccct
    17941 gtcttttata aaagtataaa ccgtacaatt cgcctggtct aaatactgat tttggacagc
    18001 cgtttcaatg gttatcgttg atatatttcc tttgatcgct gaataagcaa atacaccatc
    18061 ttttgcaata ttgacactag catatttatt cagccataca tgacgataga tgccggcacc
    18121 ttcgtaaaac catccttcat attgtgacgc atctgccctc acgacaagca cattgtcctt
    18181 atcaaaattg atatagtcgg ttatatcata ggccgctcca atataaccgc tcttattatt
    18241 tccaaggtaa aacccgttga gccagatatt ggcatcacgg aaaataccat caaactgtaa
    18301 ctgaaaccgt tggcctgaat ctgatcttgc tactgtaaaa tgcttacgat accagccgat
    18361 acttgtttcg ggaaataaac caccaacagg cttatagcca tgggccatca catcaaaatt
    18421 atcaacattc acaaatggca attcaacggc ccaatcatgt ggcaggttta attttctcca
    18481 tttgccgtca tcaaattttg gatcgatcgc tgtttttaaa gctgcaccgg atttggaaaa
    18541 tatattagca atgctgtagt taaaatcttt ctccgcattg gctgcatggc caaaatggaa
    18601 cttccagtca ttatcaaaat tcagttgctg tctttgtgca ctaactacca gtgacaagcc
    18661 agttgctatt gctaaaaaaa agcctgtata tttcatacag gcaagttagc aaagtaagta
    18721 ataaataaag tcaattgaat gcatttaacg caatcgttgg cttaccggaa ttatcaaatg
    18781 ctcctttggt atatccctgc cagtttccat agcattgtgg ctcccaataa aaaacgccca
    18841 ggcctttatt attgggaaca gattttactt tattgatcag atcagtaaga aatgattggc
    18901 atgtggctgc ctggtcccag ctcattccca cttcacagat catcaccggt ttgtcatacc
    18961 tggctatcat atcattgata ttagctaaac attgcgaatt catcgttgac cagttagcgg
    19021 gatccgggta caaagacatt cctattatat cccatttccc actgttattc tttagtccat
    19081 caaagatcca tcgaaacaag ctgttgtcat ggccatttga aatatgaaca atcactttaa
    19141 ttgttgcatc aactgctttt acggcatcat atcctttatt tattaatgca gcaaaattgg
    19201 ccatattcac cgaagccttt cctgttggcc aaagcatccc gtcattggtc tcattgccaa
    19261 cctgtaccca ctctggcgtt actccgctgg atttaagtgc agttaaaaca ttgaatgtat
    19321 gattgtaaac ggaggtttgc agtgttgtaa aatcctgtgc taaccaggca gcaggttttg
    19381 tttgctgacc cggatctgcc caggagtcgc tgtaatgaaa atcgatcatg attttcattc
    19441 ccagattttt ggcccggata gcttttgcta ctacatcagc tgtattattc cagccgcctg
    19501 caggatcaac ccacactctc aaccggatcg aattcatacc caggcttttt aatagttgca
    19561 tacattcctg tgctgttccg ctgctgttgt aaaagatccg tccgcttgat tccatttgtg
    19621 taagccagct gatatctgct cctttggcaa actgaaccgg agttacaacc ggaggtggag
    19681 tatcgttttt atccgggtca catgaaacaa agatgaacaa aacaaaagcg gtataaataa
    19741 acctgagttt gttatacaaa attatttttt tcataacaga ttgcttaaaa aaaacgccac
    19801 cctgctctga ggatggcgtt ttacttattc attcatggaa ggttagttaa tggttactgt
    19861 ctgagtatac tcattcagcg ttattgtata actacctgtc gtagccggcg ttttgaaatt
    19921 atcgcccccc tcccatttga tctttcttgg tgttccggtg gctgtacttc ctccgctatt
    19981 atcttcataa tcaaaggcac cccacgcatt gcctgcaagg aatttaatat ccttacctgc
    20041 tactaatgta agagtgattg accacacacc attaccgtta taggtcattt gcggactggc
    20101 accaggatcc catgcgttta caccctgcat accatcacca acaaccctca tttcagtagc
    20161 aggagacatt tcatatttaa tattgtctac gtcccggcca tcccacatta ccctgtaacg
    20221 tccagcagat gctacattgg ggaagttacc accattgatt tccattgtac ttccacttcc
    20281 gcctgcagca ccatagcttc ttgtatttac aactgttaaa tcgccactgt tccactggtt
    20341 attatctatc aacttccatc catcaactgt aaaatctgtt agaccaacaa ataggttagt
    20401 tgaagctgca cccatcgcat aattagggaa agtgcttgga ggatcccagc cagccccggt
    20461 tccacctcct acaaatccca tgcgaccttc cctgatatca tattgtaatc ctgttctgtc
    20521 aatggaaatt ctatagtacc cggctgttgc aacagtaaag tttgctccac caaccgacaa
    20581 tacaccagcg gttcctccat aatttacatc ccatgaacgg ccctgagtaa ctttaaattc
    20641 agcaccagca ggcagataaa tgtacatata atacaactta ttgagtgcac cagcccttaa
    20701 gtcacgaatg aactcaggtg ctgtaccagg atcccaatta ttaccattac ccgttgcagc
    20761 ctggtaacta ccgggcaaat agaaacgaag gatcggtata taactttgaa tcgttacatt
    20821 aacaacattt gaataagaaa ctgcaccctg tgcagtagtt gcttttattc tgtattcaac
    20881 ttttcctgta ttgccaccgg gaataccgga ggttaaggct gtctcattca tttcgccttg
    20941 ggtcaacgct tttgtataaa ggttggtacc aacaggaatt tcaagtggcg ctacaaaatt
    21001 ctttgtcgct gaatcatatt ggagcacata attaatatta cctgtatagc cattgaacgc
    21061 attactccaa ttaaaggtat ttgacagttg tgttgaggtg gccaatgcac aagtaactga
    21121 agtattaact gtagttaata cggccgggtc attgtatggt gtaacactaa cttttacaac
    21181 attagaaaga tacctttcat tgttattacc ataagaagat gtaactctaa catccagatc
    21241 atacggcact cccagtgaga atccataatt aagtaatatc gtgttcaact ccttaccagt
    21301 aaggctggta gtaagaaccc ctgttacaat cttagttgct tctttagcaa aatttcttcc
    21361 ggttgaatca atttccagga tgaatttata caaagtagta tcctgtttgt actcaggact
    21421 ggtccaggaa aatgagatta cagcggaaag agaatcagcc ggagtcggtg ctaccgaagt
    21481 tttagatgcc gtaagtacaa ctgcagttcc attatcgtaa tacggaagat ccgccacttt
    21541 tgtacaagcc gttaccatta gtacggtagt gacagccagt gtaagtattt taaaaatatt
    21601 tttcattgta tattttttaa tatccgtaat tatagttttg taatggtgta tttacctgtt
    21661 ttgaaactca caacaatctt gtagttaccc gccagaggag gtgatttaaa atcatttcca
    21721 cccggttgaa aactatctcc attcacatta tttccaaggc ccgccccggt aaaaccatat
    21781 ttgtttgccc aatctccata cacgggaaca aataagaatc cggcatcagg attaatcgcc
    21841 aagctggcta tttcaaactc agaagtagaa atcttggtga acttctggct taaaagctcc
    21901 ggatcgccac ctcccatcca attgcccggt gtagcagctc ctgttatata caagttagcc
    21961 ggaacagggt atacaacatc gagataaggt gtcattttta tagctatcac attagaatac
    22021 aacggcacac tattattaac aagacttgac ttaaccctta tttcaatatt atgaactaca
    22081 ttttctggaa ggtccattgc taaaagagca gcattaagat ccctgacagt aaatgtagaa
    22141 gtcagttctc ttgcaatagc cttttcatat tttttagcgc taccaaagtt ggctccagca
    22201 acatcaaact gcattgtata cgtaacatcc tgggaactga ttcctgttgt aaacttataa
    22261 tcgggattag tccagttgaa gataattgca ggattatttt cgttcacttt gagtagtatc
    22321 tgatcagctg ttgttgaggc tgtcaataca ggggctgtgc ctccctcaaa aaagatttta
    22381 ttttcatcct tctcgcagga tgagataatg aatcccagga atagaaaccc aagaagtact
    22441 ttaaacatat ttttcataat ttaatcagtt tatattgtat taaattaata accggtgttt
    22501 tgtgtcaggt tagggttaga tgtaatattg ctggccggga tcgggaaaat attatactta
    22561 ctgtcaacag cagtacccga tgcgacacct cctttccatg gccacaaata agtgcctgta
    22621 gtaagaagac cgtaacgtat caaatctgta cggcgatgtc cctcccaata tagttctctg
    22681 gccctttcat ccaaaataaa aggaagtgtt aattgggcac tggtaatatt cccggaagta
    22741 ttgctatatg ccctttctct tatcaggtta atataattaa ctgcattagc gatagaacca
    22801 ccggatccac cacgaagtac ggcttcggca taaataaggt acatttcaga caaacggaaa
    22861 acaggaaaat caacatcaga aaagtccctg ttcggatctg aggtaggagc tccatctgaa
    22921 cggatgtttc tgtattttct tacatgaagc ccattggaga aattgcttat atcatcaatt
    22981 acgatctgtg caggacttgt accaaaacta gatgtagtga acaaagctct tttatcagtt
    23041 gcaccggtta aatcagaaaa tttatctgcc aaccccttag ttgccctgta accataccaa
    23101 cctcctccta caccataatc attgtgatcg tcaccggcag cacaatggac aaaaaatgta
    23161 gtattaccat atgagcttgt acgaaggccg tcacagttta tagcataaat gatctcattt
    23221 gtccgcttat cattatcagc caaaaataat tgcgcataat tattatgcaa tgcatatccg
    23281 ccaccgatta ccttgctggc ataagtgata gcttcggtat attttgaggt gccggtataa
    23341 gtctgtgcgt tgaggtacaa cctggcaagc aaagcccagc aggcaccctg atctacccga
    23401 ccatattcaa tggtttttac aggcttcaga tcagactcta ttgctaaaag ctcactttcg
    23461 ataaaagaaa aaagttcttg gcttgacttt tcagcaggaa gagatgtacc tactaagttg
    23521 gtctcatcaa taaatgttga tttcccaaaa agatccatca tagcccaata gttaaaagcc
    23581 cttaagaaac gaacttctgc ccttgaagct ttaatagcat ccgcatcggc accgctaatt
    23641 cctcttgaag acaatttatc atctgttgat tcacgcagat actcgtttgc caccattatg
    23701 ttataaatgg gtcttgcata cattcctttc agaaaaggat cgccacttga ccatctgagg
    23761 ttatgaaaat ctttgatagt ctggtcattc caggcaacaa ctgcttcatc cgttggcaat
    23821 tcctggcaat taaaaaaacc tctgataaaa ggtgattggg aaccttcgtc cagcccctgg
    23881 atatctgatg aaccagcagg cccggtattt ccggttgtag ctaatccccc gtatacttta
    23941 gctaatacgg aacggtaacc atcggcatta gcataagctg tttctgaagt gaattcgttt
    24001 tggggaaaca aatccagctt ctttgaacag gatgctaaag aaacaatgac agcccctgtt
    24061 attaatattt ttactattga taaatgtttc ataactcaat attttttaca attaaaaatc
    24121 aaaattgaat ccaagtgaga aaatacgggg tcttggataa atattattat caacaccacc
    24181 atcacctgca ttctcaggat caagtccttt atattttgaa ataacaaata cattctgaat
    24241 gctagccgcc acccttagag aagctttatc attaaacacc cttcctacat tatatcccag
    24301 attaatattg tccaggcgga agaaagatgc attctcaata taataatcag acaaatactg
    24361 gttattaaca aaacgggtat tcagataatc tactgaagca tttccgataa aattgatggg
    24421 atttttaatc gatctgagca caccattatt agaaaagaaa ttattataca gatagttgcc
    24481 aaatgaacca tgacctgcga tgccaatact gaaatcttta taagtaacct gagtgtttag
    24541 acccagcaaa acatcggcag cgggtttttt ataaaaatat ctgtcatcat catttattat
    24601 cccatcacgg ttaacatcct cgtacaatcc ttcgatgggc ttattattat catcataaat
    24661 ctgtttgtac accaggtatg tgaagggtgt ataattaacg gcatgtattc ctacaaagtt
    24721 gcccgtacca cccgaaatac cgctaacctg ttgcccttta aaattaggat caggattttt
    24781 cagcaggtta gtaatctcag cttcattata agtatagtta atccccaggt cccaggtcag
    24841 gttttctgtt ttaattggtg tggtattcag attaatttcc actcctttac ttattacatt
    24901 tcccacattg gtagtgatgc ggttaacaaa attcgcaccg gctggtacat ccacatctgc
    24961 caacaagtct tcagtttttc tatagtaata atcgactgag cctgttatcc tgctcctaag
    25021 aataccgaaa tccaggccaa tatttgacgt ggttgtggtt tcccatttca aattgggatc
    25081 atacccttca ggtctcaaat aactaacaaa actgttacca aattgatatt gagctgtatt
    25141 ggtacctagt gaatatctgg gcaagtatga gtagtaacca attccatcct gatttccggt
    25201 ttggccccag cccaggcgaa gtttcaattc attaagcaca tttgcgtttt tgaaaaattc
    25261 atctttcaat ttccatgcaa atgccacagc agggaaatag cccaccctat cattaggatt
    25321 aagttttgaa cttgcatcac tcctgataga agcagtaatg aggtatttgt tcattattga
    25381 aaaattaacc cttgccaggt atgattccaa acggaacctt gggttgtcat ccgggaatgt
    25441 tggttctgat ccctggattg tatctttttt ggcggggtta tttgggtcag caatagccct
    25501 gtaactaaat gatggatagt tgtaaacctt agttaaaaaa tcctggtaac tatgaccaac
    25561 caatacatct attttactat ctatactttt aatattcttt tcataaaaca gaaagatatc
    25621 agcaagtgta tttacctttt tctgttcata gaatgttttc cttcctccgg ttttatagtt
    25681 agtagctgaa acggaatcaa tattatcatt tccactgccg cttaaataat caagaccaaa
    25741 gttcattttg atatgcaaat cagggaagaa gtgtagttta taatcaagat ccacattacc
    25801 aataacacgt ccgactttag aagtattatc tctgagattt aaaagaccca atgggttcct
    25861 tgtagaaaga tcgataggca gattattcgc ctgcagccac tcgtagtagc caccccattc
    25921 tttattacca gacaaaaccg gtttggttgg atcaaaggca atcgaagaac ctattgcccc
    25981 ttcattagca aatgtgttct ttgtctggct ggctttaaca gaaagattaa ctgccaaatg
    26041 attttcgaaa agctttggtg aaaggtttaa cgcagttgaa aagcgattga aattatttgt
    26101 tttcaggatt ccgtcctggt ttaaataacc taatgaagcc ctgaagggaa cattacccag
    26161 tccaccgctt acgctgatgg tattatcaat accaaaagct gattggtata tttcatcctg
    26221 ccaattagta ttagaagatc cgagaagatt tttatacgta ttatttcctg tttcagcagc
    26281 atcactattg ataatatcag ttacctggcc ggctgtaagc acctctactg ttttaccaac
    26341 tgttcccaca gaaaaaagat tattataatt gaatctcaat ttacctttaa ctcccttttt
    26401 agttgttact atcaaaaccc cattggaggc tctggacccg tataatgctg tagctgatgc
    26461 atcttttaat acggagatag attcaatgtc attcgggtta atggagttca gtacattggc
    26521 agaacctgca ataccatttc cttccacagg cacaccgtca ataacaatca aaggatcatt
    26581 actggcattt aaggaagcac caccgcgaat acggatacgg cttccacctc cggcagaacc
    26641 accaccggtt gtaatctgta aaccggctac tttaccttgc agcaattgtt ccggcgaatt
    26701 aatatttcct ttttggaaat cttttgcaga aacagaagtg acagaaccgg tcaagtcact
    26761 ttttctccgg gttccataag aaactaccac tacttcgtcc atcgaagtat tagtaataac
    26821 taatgccact tctatagaat tttttccttc aattgatact tcttttgtgg caaaaccaac
    26881 agaagaaaca attagagcat ttactgtaga aggcactgat attctaaatg ttccatccga
    26941 accggtttga gcaccactgc cgctgccttt ggcggcaaca gtagctccct gcaacggaga
    27001 accatctttt gaatcagtaa cttttcctgt aatcactcta tcctgggcaa aagaaatttg
    27061 cgccattaaa gccagcacaa taagaccggc agccttaaag agtttttggg gagacataag
    27121 caatcattaa atttttaagc aatcttattt ccaaatacac aacaacatgt ataaaagaca
    27181 catttaaatg tgtattaaac acacaatatt ataacatttt ttaagtttgc aaaaaataat
    27241 ttttaacgct ttgtaaaggt attgacgaaa atcagctgcc taaatgtttt gtttaatttc
    27301 ataactgatt gtttattaat acttaaacaa attttatggt aatcaggtct gccacaggca
    27361 ggaatatcca tatgaccaga atcaccggca atccaggagc tcacaggtat aaaccgcaag
    27421 taatcccggc aatacctggt taaagaaaaa ataaactgtt ccggattttg aactatatct
    27481 ctaccaaaat tgatcttttt ctccgtgttg aaaattttca aacccagtta tcgatcactc
    27541 tatgctgtga atattaccca tgccagcata aaaatttaaa agtttatatt gcatcaaccg
    27601 cctaccggga tttacagaat tgagaaagac tgcagccata tcattaatgc ttattttcct
    27661 ttttaattta gggggatatc aaattgtagt caggcagttg gagaaaaaag ccggcagaag
    27721 cctggaagct ctattggata aaaatgttta ttacatctct ggtctcactg aaataaggtt
    27781 gttagcagaa agccctgccc aagatcaacc agccggatat gacagctacc atgacaatat
    27841 ttatactaac acacatatca tcagcaataa aaatgaagaa actcacattg ttgtctttaa
    27901 agacattcta aacaacgggg gtcctcaatc taaggatttc cgctacgata tgaacagtgc
    27961 taaaagtacc gctggcgcat cccggcatgg tcatccaaaa caacacagtt cttttgttgt
    28021 aaacattctt ggcgaatacg ataatcagcc tgtattgtta tcacatagcc aggctgacta
    28081 cacaatcact catagttccg gttacgcttt tactataccg gcattccacg gtcaaacacc
    28141 gcaccagcca cctgattgct gatacttgct ccggccccga cattgatctc catttcttta
    28201 ttttattaaa tgataacgta tgcgtttagc gatagtggct attgcagcat tgctgcttgc
    28261 aggtgcctgt aataaaaaga acgattataa acaaataacc agcaatcctc ttttatattc
    28321 ccggacagtg aaagaactca ataatgtggt gatgcaaaac aatttccccc ccattattgc
    28381 ttcaagaaac tacctgtacg ccaatattgc agcatacgag atcattgcag caggcaattc
    28441 aaccaggtac agttcgttgg ccgggcaagt aaagcacctg acaaaaattc ctgcgcctcc
    28501 tgttggaaag gaaattgatt tcaactttgc tgctatcctg gctttttgca agttaggaga
    28561 agctgtcact ttcccggaag gaagtatgaa agaatatgta gacagcctga agacgcttgt
    28621 aaaagaacat ggtatgcccg ggagtgttta caaaaacacc gtggcttatg ccgaagaagt
    28681 aggaaaggcc atcctggcgt ggagcaagaa agataattat gcccaaacaa gaacagcccc
    28741 caaatatacg gtaatggata ctcccggaag atggatacct accccgccga tgtatgcgca
    28801 ggcactggaa ccaaattgga gggagatcag aacaatggtg atggacagtg ctaaccagtt
    28861 tacgtcacca ccacctcctc catttgatat cagcaataaa caaagcaaat attaccagga
    28921 agtaatggcg gtaaaaaatg caggagacag ccttacggat gaacaaaaac atattgccga
    28981 attctgggat gatctgggaa caaggctgaa tgtatcaggc catgtagaat ttctgacaaa
    29041 aaaattctca ccaacaggtc actggatgaa tatcgttggc atcgccgcaa agactgccaa
    29101 cgttgatttt cccaccaccg tttatgcgta tgcaaaaaca gccattgcta tgttcgatgc
    29161 gtttattcag tgctgggatg aaaaattcag gagtaataca gtacgtcctg aaacagtaat
    29221 caataaatat gttgatcctg actggagacc atacctgcaa acaccgcctt tcccggaata
    29281 cacttgcggt catagtaccg tttctgcgtc ggcagcagaa gcattaacca gcgtattcgg
    29341 tgacaatttt gtttacacag atacatctga actggagttt ggcatcaaga accgttcatt
    29401 cacttctttt cgacaggcgg cagaagaaaa caattgggcc cgtttttacg gaggtatcca
    29461 ttttcataat tcctgtatca taagtacgga atatggaaaa aaagttggag acctggtgaa
    29521 ccagcgtctc caaatgaaaa aataattgat aacaaaaacc aacgattaaa aatgaa
//
LOCUS       DAORTI010000031        28932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1179_length_28932_cov_6.835232, whole genome shotgun sequence.
ACCESSION   DAORTI010000031 DAORTI010000000
VERSION     DAORTI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28932
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_28932_cov_6.835232"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PLO70_06025"
     CDS             complement(<1..2008)
                     /locus_tag="PLO70_06025"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQZ74051.1"
                     /translation="MVFDLDLIKKLYSEMPAKVDDARKTLGRPLTLAEKILYAHLWKG
                     VDVKEYERGKAYVDFAPDRVAMQDATAQMALLQFDTTGRKMVAVPSTVHCDHLIVAKV
                     EGKADLNKAVTDNEEVYNFLASISNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLSWELLMPKLIGVKLTGKLNGWTSAKDVILK
                     VAGILTVKGGTNAIVEYFGEGAQSMSCTGKGTICNMGAEIGATTSTFGYDESMSRYLK
                     ATGRAAVAEAADKIKECLTGDAEVYANPEKYFDRVIEINLSELEPHLNGPFTPDLATP
                     ISQMKEAAAKNGWPTKIEVGLIGSCTNSSYEDISRAVSLAKQVKAKGLKLKSEFTITP
                     GSEQVRYTIERDGFLNVFEEIGATVFANACGPCIGMWDRMGAEKQEKNTIVHSFNRNF
                     AKRADGNPNTMAFVASPELVTALAIAGDLNFNPLTDTLTNDKGEQVKLDPPNGDELPV
                     KGFAVEDAGYQAPAEDGSKVQVAVKADSKRLQLLYPFAAWEGTDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKFRGHLDNISNNLLIGATNFFNEKTDNVKNQLTGEYGTVPNTQRAY
                     KAAGIGTIVVGDENYGEGSSREHAAMEPRHLGVRVVMVKSFARIH"
     gene            2215..2436
                     /locus_tag="PLO70_06030"
     CDS             2215..2436
                     /locus_tag="PLO70_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HQZ74052.1"
                     /translation="MNKFKNFVEWNAFGVCSAIGNRLGIATSKIRQYFMYASILTMGS
                     PIIIYMVLAFFRNVKKYVIAAKRNPWYYL"
     gene            complement(2524..4419)
                     /locus_tag="PLO70_06035"
     CDS             complement(2524..4419)
                     /locus_tag="PLO70_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQZ74053.1"
                     /translation="MPAAFIYFLKLSVSLGVVFLFYQLVLRKLTFYNWNRWYLLGYTL
                     LSFIIPLIDVTPALHQNELTGSTMVQWVPLLYKQSAGGTSLTVWNILFLFIISGMLVM
                     LIRLIIQLVSFRGMMKKAVPLNIGPDSAINLYQVNESIIPFSFGNAVFINRHLHTAEE
                     LEEIIRHEFVHVKERHSIDIILGELLCLFNWYNPFAWFLKSAIRQNLEFVADNKVLEN
                     GINKKQYQYLLLKVIGNNQFSIAQKFNFSSLKKRIAMMNKNKSAKMHLLRFLFVLPVL
                     AFILISFRKEIGDTLTGKQEQIQTLAATYATDTIPEVTVPNNKGYFINVKDRKGECLL
                     VIKDKTGKEVKRLLLTEWNANAEKYEEKYGELPPPPPPPPPPVPAIPGQADVITVQGH
                     PIPPVAPNGEVVVMGYPNPPKPVKLPEHVKSINIKNGGKAIVELKNGKTEEYDLNNPE
                     EKKKFDARYPAPPPPPPPPAPGRTVVVEGHKMNGSVSTIAADGQPLPDNLVYVINGVI
                     STKNEMDKISPDKIATVDVLKGNSAIALYGEIAKDGVVQVRLKNEAHPLYVIDGVISS
                     KADADKINAESILTVDVLKGEAAEKQYGGKGKNGVIIIRTNRKEVQKPVTISSFLFPA
                     EVKKDQC"
     gene            complement(4449..4811)
                     /locus_tag="PLO70_06040"
     CDS             complement(4449..4811)
                     /locus_tag="PLO70_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQZ74054.1"
                     /translation="MEKLTHTEEETMQAVWRTGEGNVKAFMENMDEPAPYTTVASIIK
                     NLEKKGYLNSRLVGNVYMYKPAISEDDYKQKFMGNVVKEYFDNSYKELVNFFVEQKKL
                     SAKELKEIITLIEKGNNK"
     gene            complement(4947..6074)
                     /locus_tag="PLO70_06045"
     CDS             complement(4947..6074)
                     /locus_tag="PLO70_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQZ74055.1"
                     /translation="MNRIYFDNAATTALDKEVLEAMLPYMTTHFGNPSSIYSYGRESR
                     LAIENARKTVAKILNANPGEIFFTSGGTESDNMAIASAIHDLGCRHIITSPVEHHAVL
                     HAVEHFDHDDVVTSSFVKVLPDGHIDIGDLENQLASREERCLVSLMHANNEIGNLLDI
                     DAVGEICKKYNAIFHSDTVQTIGHYKIDLRSKPVHFAAGAGHKFHGPKGVGILYINDN
                     IKVKPMIFGGGQERNMRAGTENLYGIVGFAKALEIADTHMEKESASIQSLKTYMIEQL
                     RKHIKGVRFNGDYAGKSLYTVLNASFPKTEKSEMILFNLDMQGICVSGGSACSSGADV
                     GSHVIRAISNDPNLVPVRFSFCKHNTKEEVDVVVSKLKELI"
     gene            6243..6860
                     /locus_tag="PLO70_06050"
     CDS             6243..6860
                     /locus_tag="PLO70_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQZ74056.1"
                     /translation="MEQKQDHCCNGGSRRNFIKKTTALTALSIVPSAIIKAGEHHEAI
                     ASYMEKTPVQLEINGKPYKVLVDPGVSLLDMLRENVGLTGTKKGCDQGHCGACIVHVD
                     GKRVNSCLSEAVDNNGKKITTIEGLAEGDKLHPMQEAFIKHDAFQCGYCTPGQIMSGV
                     ACIREGQAGSPDEIKEFMSDNICRCGAYPNIVAAITEVKNSGQKI"
     gene            6907..9132
                     /locus_tag="PLO70_06055"
     CDS             6907..9132
                     /locus_tag="PLO70_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQZ74057.1"
                     /translation="MNNKIKFFQTDNDDDRVDGIDKVTGRAKYTADHMLENMAYAVFV
                     TSTVAKGAIKSMLLHEARNAPGVLDIIYYQNCPPVPGYRPYAKDPAKKGFEWRGLKVF
                     DNNLVYYYGQPIALVVATTWEKAVKAASLVKAEYITEAFETDFETLRKNEKKLKPAGQ
                     YKRGEEDAWKNAEVSIEAEYFTPIETHNPIELHATIAVWEGDDKLTVYDKSQGPKGTQ
                     SELARNFGLDEKNVRVITKFVGGAFGSALRSWPHVPAACIAAKVVKRPVKLVLNRRQM
                     FTMVGYRPQSWQKISIGATKEGKLTGITHHAISNTSRYEDFREGIVNAAQFLYECPNV
                     NTSYKILPLDINTPTWMRGPGEATGAFALECALDELAYKLNMDPVELRIKNYAEVHPV
                     SKLPWTSKFLKECYAAGKEMIGWHKRPSIPRSLQENGMLVGYGMAGGVFGAGRGNATV
                     KAILKNDGTLILQSAVSDMGPGTATTMVKVAAEVMQMPASKIKFQLGDTDFPPGPTQG
                     GSTTTSTLGSGVSIACEALKQALKELARTIIPSFSTIAAEELGIADGYIVSKKDSQQK
                     ISFTELLQKAGKPEIEVIKESTRATAEFQKYAMNSFSAHFVKVHVHPTTGAIALKQII
                     ATGDGGKIISEKTARSQMIGGAVGGIGMALTEEALIDHTTGKYITDTLGKYQVPRHRD
                     VPFIETYFPNKPDPIINPMGSKGVGEIALVGFAPAVANAVFNATGKRVRSLPIKPEHL
                     I"
     gene            complement(9263..10642)
                     /gene="glmM"
                     /locus_tag="PLO70_06060"
     CDS             complement(9263..10642)
                     /gene="glmM"
                     /locus_tag="PLO70_06060"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQZ74058.1"
                     /translation="MALIKSISGIRGTIGGKPGENLTPLDVVKFTAAFGTIIATGKQS
                     AKIVIGRDGRISGAMVQNLVVNTLTGLGIDVIDLGLSTTPTVEVAVKMENADGGIILT
                     ASHNPKEWNALKLLNGEGEFISAEVGERVLELAAREDFSFATVDKLGTVTADDTYLQK
                     HIDAILKYPLVNAKAIAKQDFKVVVDAINSTGATYVPALLKALGVTNVIVLNGEVNGK
                     FAHNPEPLPEHLSQLCNEVIQQKAHLGIAVDPDVDRLCFVNEDGTLFGEEYTLVAVAD
                     YVLSKRKGNTVSNMSSTKALKEITLKHGGEYFPSAVGEVNVVKKMKETNAVIGGEGNG
                     GIIVPDFHYGRDALIGIGLFLSHLADHKKGIKSLRTTYPDYFISKNKIELHNGVDVKA
                     IFEKIKTKYKNNPINMEDGLKIGFDNDWVHLRTSNTEPIIRIYAESDFETKANNIAMQ
                     LMRDIKEFS"
     gene            10720..11517
                     /locus_tag="PLO70_06065"
     CDS             10720..11517
                     /locus_tag="PLO70_06065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ74059.1"
                     /translation="MAEKAWYKEWFNSSFYHKLYFERDEKEAEAFIKKLVQHLHPAPG
                     SRMLDVACGKGRHSKTLASLGFTVTGIDISGDSIAYARQFEKDNLDFFVHDMRLPFWG
                     NYFHYAFNFFTSFGYFKTRREHDNAIRTIARSLKPGGQFVIDYLNVHYAEEHLIYNES
                     KVISGTNYAIHRWEDETHFNKKITVTDAALKQPLEYTEKVAKFSLGDFTDMLSYQGMQ
                     VQEVFGDYQFNSYDVRKTPRLIVIAEKKSNHSDYSSSNTLTSHKRQW"
     gene            complement(11459..13486)
                     /locus_tag="PLO70_06070"
     CDS             complement(11459..13486)
                     /locus_tag="PLO70_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQZ74060.1"
                     /translation="MYSFIIAIITFEGQKLMLVKYKIPKTILWVANLFVIFLLIFTLF
                     RLATFFAFKPKGISFGDVVPSFLMGFRYDLRWIAIILMPIVLFSTFPRLSPFYSSRNK
                     KWWTWYLAIVTFIVFFFFAAGFGSFSYNRTPLDAGAMNFAEDFSISLKMIWQTYPLIW
                     MLLGLLVAVLFFRWMYHRSHWQVINRTEGLGITHRRKFFIITVILLCFFIYGSLSWPP
                     LSRSDSFRYNNSFKSYLAINPLQNFVATLKLRKPDYNERKAREVFPVMAEWMELPDKS
                     DFSFRREIGPRSSSFESRPNIVLVQCESFSMYKSTMSGNPLNATPFFDSLSRQGIFFE
                     RCFSPHFSTARALFAILSGIPDAQLFKFSSRNPQAVQQHTIVDNLEDYEKHYFLGGSP
                     EFNNFEGLLKNIDGLQMHTGDYFKAPKVNVWGISDKDLFLEANELFRKKDKPFFAYIQ
                     TSGNHRPYNKTIPESDTDFVRVVVPEEELIKYGFESLDEYNCFRYSDYCFRMFMEAAR
                     KENYFHNTIFVFVGDHGLAGNAEAMYPPVWTEQRLTDEHVPLLFYAPDLLVPQRRSEV
                     VSQIDVLPTIAGMLHQSYVNTSLGRDLLDPGKKNNFAFITNTADGIGMVTDDFYFTRN
                     INSGEEQLNALHSGVLNYTTAKKDSVRKKLSVFTTAFYETSKYLMMNNQND"
     gene            13581..14198
                     /locus_tag="PLO70_06075"
     CDS             13581..14198
                     /locus_tag="PLO70_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQZ74061.1"
                     /translation="MPFLAINIWEKLVLWDKWLFIQINSQWTNPLFDQIMPLWRSPVL
                     WAPLYLFLLVFVLMNFKVKGLWWSLLFICSIALTDMVGNYVFKHGIERIRPCNDPDFY
                     MHVRLLLEHCGVGYSFISNHAANHFGMAVFGFITLRHIIGNWAWIGIAWALSVAYAQV
                     YVGVHYPLDVAGGALLGVIFGVTTGTIFNKRFRFAIFDNQSIPAS"
     gene            14198..15493
                     /locus_tag="PLO70_06080"
     CDS             14198..15493
                     /locus_tag="PLO70_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQZ74062.1"
                     /translation="MDIFILGVLILVNGLFSMSEIALVSARKARLEHLAEKGDKKAKL
                     ALELSNHPELFLSAVQIGITLISIVTGVYSGERFSKNLQPVFENIPLLQPYAETISTT
                     LIIILVTFLSIIFGELIPKRFGLLRSEKISKAVAGPMKTFGTITHPIVWLLNRTSNIF
                     FTLFNIKRSKEDAVTEEEIKTLITEGAEAGTIDEAEQEIIERVFHLGDRNITSLMTHR
                     SDIIWFNLDDNEDKIKEKILQETHSAYPICDGSIDNIKGVVSIKDLYISPDNTLFKEI
                     MQPALFIPENNSPYQVMEKFKESKIHSAFIVDEYGSILGLLTLNDILEAIVGDIPQQN
                     VPDYEIIEREDGTYIVDGQIPFYDFLTRFEKTEWMNEGEHDFDTLAGFILHELERIPK
                     TGDKLNWKGFAIEIIDMDGHRIDKVLVTISKELREEMEE"
     gene            complement(15500..16033)
                     /locus_tag="PLO70_06085"
     CDS             complement(15500..16033)
                     /locus_tag="PLO70_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74063.1"
                     /translation="MKNIVSMAASVYKWSIIPLVAIILMAARPVIVHPFHVSVIEINH
                     NAVDKTLEISCKIFTDDFEKILAKNYKAKVDLINPPNKAAMDSLVKKYLFSHLSIKVN
                     GKPVLFSYLGFENDKEAAYSYIEVQNVPVVNKLEVTTDIMYDQFDDQMNIMHVTVNGN
                     RKSSKLNFPDAGAVFIF"
     gene            16123..16701
                     /locus_tag="PLO70_06090"
     CDS             16123..16701
                     /locus_tag="PLO70_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQZ74064.1"
                     /translation="MSDFNFYFGLGWEHIMSWDALDHLLFIAALAAIYLLKDWKQVLI
                     LVTAFTLGHSLTLALSVMDVFRFPSNWVEFLIPCTIVVTAISNLFQKKFTTKSIRINY
                     FLALFFGLIHGMGFANTIRFMLANDQSFGWGLFGFNVGLEAGQIVVVSTLLLVTQVLI
                     NIAKINRREWVIFLSAAIFSLALKIAIERLPI"
     gene            16716..19100
                     /locus_tag="PLO70_06095"
     CDS             16716..19100
                     /locus_tag="PLO70_06095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ74065.1"
                     /translation="MKKIWILCCLLLPFTIATKAQIQNNASSNHANKFEQLGTILPTP
                     NEQRTASGAPGTKYWQQRVDYDIKCELDEVNNKLTGSETITYFNNSPDVLNYFWMQLD
                     ENQHSTTKNADYQSSNRLPNQTVDKMLDRFEEKRSDNGYGVNITKLIDAAGKPLKYTI
                     NKTMMKVDLPAPLKPGQKFVFKVDWNYKLANRGDFMRFGGARGGYEHFAEDGNNNYTM
                     TQWYPRLCKYSDDQGWQNHQFTGTGEFTLCFGNFKVQMTVPADHIVGATGECQNYAQT
                     LTATQMARWQKAQTAKEPLQIVTLEEALAASKKTSNSAKKTWIYKADNVRDFAWTSSR
                     RFVWDAMATNVEGKKVMSMSYYAKEAYPIYSKFSTKAVAHTLKTYSKFSFPYPYPVAI
                     SVEGNQGMEYPMISFNPGRAEKDGTYTEGAKNAAILVIIHEVGHTFFPMIVNSDERQW
                     TWMDEGLNSYLQYLTQELWDNKFPSDGGPAWTITNYMKLPKDQLEPIMTNSENINNFG
                     ANAYDKVATGLNILRETIVGRELFDNAFREYARRWAFKSPTPADFFRTIEDATGEDLD
                     WFWRGWFYGTDACDIAIDTVKHAVPDVTAVPSQTKDTTVMRGLPKPDVNPFEDISKIR
                     NKQDKNIVFETDKDTSLRDFYWKYDREIAKYDTAKYPVVVRAQTDALDEEGRQKYGSK
                     HLYEISFTNKGGLVMPIILEWTYKDGTKEVERIPAQVWRKNEKNVIKTFMKDKEVASI
                     KLDPFRETADIDESNNTYGNIKEPGKFKLFKQKQNVTPAPGINPMQKAQEKKGF"
     gene            19271..>21000
                     /locus_tag="PLO70_06100"
     CDS             19271..>21000
                     /locus_tag="PLO70_06100"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ74066.1"
                     /translation="MRRFITCLFLALGLSVTSFAQNIYNNPNSNHGNKFEQMGGTLPT
                     PNEYRTASGAPGPRYWQQRVDYDIKCELDEKNLKLNGSETITYFNNSPNELVFLWLQL
                     DENQHSSVNNANYQTSNTMSRQTSPQQLDRIDEAKTDNGYGFNIMKLTDAAGKPLKFT
                     INKTMMRVELPAPLKAGQRFIFKLDWNYKLADRMGRGGRGGYEYFPEDGNHLFTMTQW
                     YPRLCVYSDFQGWQNHQFTGRGEFALTFGNFKVQMTVPADHVIMSTGECQNYPAVLSA
                     AQLARWNKAKNATEPMEVVTLDEAKAAEKQKSQNKKTWIFKADNVRDFAWGSSRKFIW
                     DAMATKVEGKKIMCMSGYPKEAYGLYRKFSTKAVEHTIKTYSKFTIPYPYPVAQSIEA
                     ANGMEYPMICFNFGRTEKDGTYSEGTKNGMLGVIIHEVGHNFFPMIINSDERQWSWMD
                     EGLNTFVEYLAEELWDNKFPSRRGPAAFITDYMKLPKDQLEPIMTNSENIVQFGPNAY
                     SKPATGLNILRETIMGRELFDYAFREYARRWAFKHPEPADFFRTMEDASGEDLDWFWR
                     GWFFSTEVCD"
     assembly_gap    21001..21100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21101..21527
                     /locus_tag="PLO70_06105"
     CDS             <21101..21527
                     /locus_tag="PLO70_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HQZ74067.1"
                     /translation="YEVKIPGSKPEGLNDAEKEKYGNKNMYELTFSNKGGLVMPIILE
                     WTYKDGTKEVERIPVQVWRLNENKVIKTFVKDKEVASIKLDPMRETADINESNNNWNT
                     IPEPSRFTIFKQTQGGGRRGGGGQGGNPMQKALENKKGF"
     gene            21656..22477
                     /locus_tag="PLO70_06110"
     CDS             21656..22477
                     /locus_tag="PLO70_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HQZ74068.1"
                     /translation="MKRISCLLFTLLLSYCIKAAAVDTITIYSTAMQKEYKCVVIQPT
                     AMPDKNTPLPVVYLLHGHGGWYANWIIRVPQLKDYADQYQLMIVCPDGGYNSWYLDSP
                     LDSTVRFETYVSKEIPAYIDAHYNTIKDRKARAITGLSMGGHGGLFLGFRNAERFGAC
                     GSMSGGVDLQPFPKNWQLAQKIGDTLNYAANWKNYSVINVIENYPEDSVAIIIDCGNE
                     DFFINVNHALHEKMLKLKIPHDYIERPGKHDWNYWRNAVQYQLLFFRNYFENSRK"
     gene            complement(22482..23198)
                     /locus_tag="PLO70_06115"
     CDS             complement(22482..23198)
                     /locus_tag="PLO70_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74069.1"
                     /translation="MRLIPLLLLFISINSFGQWKSFTLNKHGDTLNRVDMDGRKQGPW
                     SVRVDDLRGERGYEAEGYYENDQKEGTWKKYSLEGVKIAEENYRWGKLNGRQQYFTYN
                     GGLLRIENWRAMDPANKFDTVAVYDLRDTDKEIDRVVVKNEGVAMKHGKWIYYDPREG
                     TIEATENYVMNKLQTEDGGMLDDENIKPLSVSDGKTKTDTAGKKTVTKPQAILDYEKK
                     NSGKKKIRVRDGSTGNLPKP"
     gene            23293..25950
                     /locus_tag="PLO70_06120"
     CDS             23293..25950
                     /locus_tag="PLO70_06120"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74070.1"
                     /translation="MSVLLKKMGYNTRIFLPFVVILVNLHLATSVFAQVPPTPAAERL
                     KVTEQRKALESKSVLNEIKFRNIGPTIMSGRVVDIDANPEDPTEFYVAYATGGLWHTT
                     NNGQSFTPIMDNLDLLFIGDIAVNWTSAPRTIWVGTGEVNSSRSSYAGMGIYRSADNG
                     KSWEYLGLPESHHIGKIQLHPTDNNIVWVAALGHLYSANKERGVYKTTDGGKTWKQTL
                     LVDDNTGCVDLDINPSNPKEIYAAMWYRVRRAWKFEESGSSSGIYKSNDGGDTWKLVS
                     PAGSGFMTGDKIGRIGITVYPKNPNIVYAVVDNNTPKPAVVNKNDSTYKKEEFKNITK
                     ERFAQMSSKWLDTFLRANRFPRKYNAAVVKELVAADKIKPTALWDFLDSDDGFQNTGI
                     AGCEVYRSDDAGLSWKKTNEKPIGIFNTYGYYFAKIYTSHYNPDKVFILGFYAEVSTD
                     GGKTFKTMDKGNVHADHHALWVNPKRDSHVINGNDGGANITYDDGANWFKANTPPLGQ
                     YYAVTTDDAKPYNVYGGMQDNGSWWGPSNHKEDIGWIDNGQYAYKMINGGDGMQAQVD
                     TRDNATVYSGSQFGVYGRYNKDRRGAQKSIRPQHELGYKPLRFNWQTPILLSKHNQDI
                     LYYGSNRLYRSLNKGDTMIAMSNDLTNGRVSGNVPYGTLTTISESPLRFGLIYTGTDD
                     GNIHRSFDGGYSWEQLNQTTTAAPVKKGTKPPATNTKLNTNQLWVSRVTASQYKEAKV
                     YASLNGYRFDHFAPYLYVSEDHGNSWKPIGKDLPNEPINVVKEDPKDENILYVGTDGG
                     LYVSFDAGNSFMLWNGGLPKSVPVHDIAIQQRDNELVLGTHGRSLYVTKLDDVHGIKK
                     DPDWLKKKPKEKTQETRRPADNNESDPEM"
     gene            complement(26118..27401)
                     /locus_tag="PLO70_06125"
     CDS             complement(26118..27401)
                     /locus_tag="PLO70_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74071.1"
                     /translation="MKLPVEYMTAGEALAVIKSGQRVFIQGSAQTPLYLLRELAKRAP
                     DLRDVELTFITVHGDIELDKPQYADAFKINCMFVSESVRNAVNEGRADFVPVFLSDIP
                     DLFRKQMQIDVALVQVSPPDQHGYCSLGVSVDVARSAVNTATHIIAQVNPKVPRTHGD
                     SLVHTKRFTAMVYHEEDLPEVDYGTKVREDELKIGQLIAGLIEDGSTLQMGIGTIPDA
                     VLRSLHSHKNLGVHTEMCSDGIIDLFDNDVINNSKKRIHPNKTVTGFAVGTRRLYDYV
                     DDNPAFVFLDIDYVNDPHVIRRNPKVVAINSAIEVDITGQVCADSIGTKQYSGIGGQM
                     DFMRGAALSEGGKPIIALTSRTTKGVSRIVPFLKPGAGVVTTRGHIHYVVTEFGVAYL
                     FGMNLRQRAKALIEIAHPDDREILEKACFERFKVF"
     gene            complement(27476..27895)
                     /gene="aroQ"
                     /locus_tag="PLO70_06130"
     CDS             complement(27476..27895)
                     /gene="aroQ"
                     /locus_tag="PLO70_06130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQZ74072.1"
                     /translation="MKIAIINGPNLNLLGKRETDIYGNMPFEQFFDSLKKKYGSIEIS
                     YFQSNVEGELINELQRVGFEYDGIVLNPGGYTHTSVAIGDAIAAIKTPVVEVHISNVH
                     AREDFRKLSHVSGKAAGSIFGLGLKGYELAIDWLIAQ"
     gene            complement(27967..28503)
                     /locus_tag="PLO70_06135"
     CDS             complement(27967..28503)
                     /locus_tag="PLO70_06135"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQZ74073.1"
                     /translation="MFPELITDRFILKQILPADQAFIFKGLSDPKVIPFYGVRYDSFE
                     ATKNQMEFYDELWRNKTGCWWKIVSKTTDKPVGACGMNAYQPEHEKAEIGYWLLPDHW
                     KLGIMPEVIPVMIAHLFSHWKLHRLEAVIEAGNEASCRLSEKLGFTYEGTLREAEIKD
                     GKHISLMIYSLLAAERVQ"
     gene            complement(28630..>28932)
                     /locus_tag="PLO70_06140"
     CDS             complement(28630..>28932)
                     /locus_tag="PLO70_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HQZ74074.1"
                     /translation="PTFKMHHGHRGLNHPVKNIISGRSEITTQNHGFGVDPEAVKKAS
                     HIEITHVNLNDQSIEGIRVKGKPAFSVQYHPEATPGPHDSRYLFDEFIALIRSQQN"
BASE COUNT         8595 a         6276 c         5965 g         7996 t
ORIGIN      
        1 catgaatgcg ggcaaaagat ttcaccatca ctacacgaac accaagatgc cttggctcca
       61 tggccgcatg ttccctggag gatccttcac cgtagttttc atcaccaacg acgatagttc
      121 cgataccagc tgctttataa gctctctgtg tattggggac tgttccatac tcacctgtca
      181 actggttctt tacattatca gtcttctcat tgaagaaatt agtagctccg atcagcaggt
      241 tattactgat attatccagg tgcccccgga actttaacca cggaccagcc atagaaatat
      301 ggtctgttgt acatttccct ttcgccttga tcagtaattt caaccccttt aaatctgttc
      361 cttcccatgc agcaaacgga tacaacaact gaagacgttt actgtcagct ttcacggcca
      421 cctgcacttt acttccatct tctgccggtg cctggtaacc ggcatcttct actgcaaaac
      481 ctttaaccgg taactcgtca ccgttgggcg gatccaattt tacctgttcg cccttatcat
      541 tcgtcaatgt atcagtaagc gggttaaaat ttaaatcacc ggcgatagcc aatgctgtaa
      601 ccagttctgg cgaagccaca aatgccatcg tattgggatt gccatcagct cttttggcaa
      661 agttgcggtt gaaagaatgg acgatggtat tcttttcctg cttttctgcc cccatcctgt
      721 cccacatacc aatgcagggt ccgcaggcat tggcaaatac tgttgcgcca atttcttcaa
      781 acacattcag gaaaccatct ctctcaatcg tataacgaac ctgttcggaa cccggggtaa
      841 tcgtaaattc tgatttaagt tttaatcctt ttgcttttac ctgcttcgcc aaactaacag
      901 cacggctgat atcctcatac gaagaattag tacagctacc gatcagtccc acttcaattt
      961 tggtaggcca tccgtttttt gctgcagctt ctttcatttg agagatagga gtagccaaat
     1021 ccggtgtgaa aggaccattc aggtgtggtt ccagttcact aagattaatt tcaataacac
     1081 gatcaaaata tttttcagga ttagcatata cctccgcatc accggtcaag cattccttta
     1141 ttttatccgc agcttcagct actgctgcac gaccggttgc tttcaggtag cggctcatgc
     1201 tttcatcata tccgaaggtg gaagtggtag cgccgatctc agcacccata ttacagattg
     1261 tgcctttacc cgtacaactc atactttgag caccttcccc aaaatattcg acaatagcat
     1321 tggttccacc ttttacggta agtataccgg ctacttttaa gatcacatct ttggcagatg
     1381 tccatccatt cagtttaccg gtcagtttta ccccaatcag cttgggcatc aataattccc
     1441 agctcaatcc agccataaca tcgcaggcat cagctcctcc cacaccaatg gcgatcatgc
     1501 ccagtccccc tgcatttacg gtatgagaat ctgtgccgat catcatcccc ccgggaaatg
     1561 catagttttc cagcactacc tggtgaatga tgccggcacc cggtttccag aaaccgatgc
     1621 catatttatt agaaatagag gccaggaaat tatatacttc ttcgttatct gttaccgctt
     1681 tgttcagatc agcttttcct tcaacttttg ctacaatcag gtggtcgcaa tgcaccgttg
     1741 aaggtaccgc taccatcttg cggcctgttg tatcaaactg cagcagagcc atttgtgccg
     1801 tagcatcctg cattgctaca cgatcggggg caaaatctac ataagccttc cctctttcat
     1861 actcttttac gtccactcct ttccagagat gggcatatag tattttctct gccagtgtaa
     1921 ggggacggcc cagtgtttta cgggcatcat ccactttggc aggcatttcg ctgtacaatt
     1981 ttttgatgag gtcaagatcg aataccatag aatttgacaa tttgacgatt tgtaaatgaa
     2041 acaatgtgtt gcataaagca attcctgtca ttcctgtaag atgccacccg aaaaatattg
     2101 gcaaaatgac caattgccgg attaacacat tgaaaagtgt cgcaaaaata cgaaaatcac
     2161 ctatctgaat aactccgggt tgcagagatt gttcaaataa tctaaattag cagtatgaac
     2221 aaattcaaaa atttcgtaga gtggaatgcc tttggcgttt gttctgctat tggcaaccgt
     2281 ttaggaattg ccaccagcaa gatccggcag tattttatgt atgcttccat actgacaatg
     2341 ggctcgccga ttatcattta tatggtactg gcttttttca gaaatgtgaa aaaatatgtg
     2401 atcgccgcaa aaagaaatcc ctggtattat ttataataga atccgaccag ttatcgtcat
     2461 tctcatattc cccaaaaaat aaaaggccgg attattccgg cccctaggta gtttataaaa
     2521 aattcagcat tgatcttttt tcacttcagc cgggaagaga aatgaagata ttgtaactgg
     2581 cttctgtact tctttcctat tcgtccggat gataataacg ccattctttc cttttcctcc
     2641 atattgtttt tctgctgctt ccccctttaa aacatccaca gtcagaattg attcagcatt
     2701 gatcttatca gcatccgctt tggaagaaat aactccgtct attacataca agggatgggc
     2761 ttcatttttt aatctgacct gcactactcc atcttttgca atttcaccgt ataacgcaat
     2821 ggctgaattg cctttcagca catcaacagt tgcaatctta tccgggctga tcttatccat
     2881 ctcattcttt gtggaaataa ctccattaat cacataaacc aggttatctg gcaatggctg
     2941 accatctgct gcgattgtac tgactgaacc attcatttta tgaccttcca ctaccacggt
     3001 tcttcccgga gccgggggtg gcggaggagg cggcggtgcc gggtaacggg catcaaattt
     3061 tttcttttcc tccgggttat tcagatcata ctcttccgtt ttcccgtttt ttagttcaac
     3121 aatagcttta ccgccgttct tgatattaat tgattttaca tgttccggca gtttaactgg
     3181 cttaggaggg ttaggatatc ccatcactac tacttctcca ttgggcgcta cgggaggaat
     3241 aggatgtccc tgaactgtta tcacatcagc ctgacccggt atagcaggaa ccggcggagg
     3301 gggtggcgga ggcggaggcg gtaattcacc gtacttctct tcatacttct cagcatttgc
     3361 attccactcg gtcaataata gtcgtttcac ctctttaccg gttttatcct taatcaccag
     3421 caggcactcc cctttcctgt cttttacatt aatgaaataa cctttgttgt taggaacagt
     3481 cacttccgga atagtgtctg ttgcatacgt tgcagctaag gtttgtattt gttcctgttt
     3541 ccctgttaat gtatccccga tttctttccg gaaagaaatg agaataaatg caagcaccgg
     3601 caacacgaac aggaacctga gcaggtgcat ttttgctgat ttgtttttgt tcatcatggc
     3661 tattcttttt ttgagtgacg aaaaattgaa cttttgggct atactgaatt gattgttgcc
     3721 aattactttc agcaacaggt attgatattg ttttttgttg atgccgttct ccagcacttt
     3781 attgtcggca acaaattcaa gattctgacg aatggctgat ttcaagaacc atgcaaaagg
     3841 gttgtaccag ttaaagaggc ataacagttc tcctaaaata atgtcaatgc tatgcctttc
     3901 ttttacatgc acaaattcat gccggataat ctcttccagt tcttctgctg tatgcaggtg
     3961 acggtttatg aacacggcat taccaaaaga aaaagggatg atagattcat ttacctggta
     4021 aagattgatc gctgaatcag gcccgatgtt cagcggaaca gctttcttca tcatgccgcg
     4081 gaatgatacc agttggataa tcaaccgtat cagcattacc agcataccac tgataataaa
     4141 aaggaataaa atattccaga cagtaagtga tgtaccgcct gctgattgtt tatagagcag
     4201 gggaacccat tgcaccatgg tactaccagt caattcattc tgatgcaacg ccggggttac
     4261 atcaatcaat gggataatga atgaaagaag cgtataaccc aacaggtacc atcggttcca
     4321 gttatagaat gtaagctttc tcagcactaa ctggtaaaat aaaaaaacta cccccaggct
     4381 tacagaaagc tttaaaaaat aaatgaatgc ggctggcata gaactaaatt gattggttaa
     4441 accaaaaact atttattgtt tcctttttca atgagggtga ttatctcctt caattctttt
     4501 gcagatagct ttttttgctc gacaaaaaag ttcaccagtt ctttatatga attatcaaaa
     4561 tattccttca ccacattacc catgaatttc tgtttataat catcttcgct gatggccggt
     4621 ttatacatat acacattacc aaccaggcgg ctattcagat agcccttctt ttccaggttc
     4681 ttgataattg atgctacggt agtataagga gccggttcat ccatattttc cataaaggct
     4741 tttacattgc cttccccggt gcgccaaaca gcctgcatag tttcttcttc agtatgggta
     4801 agcttttcca tttctacgaa attttcgtaa atctacggac ttttcgtaga acgccaaatt
     4861 tatttttcct aaaggttttg aaacacagag gcaaaaagac acgagggaac tgagctatgt
     4921 gcctatgatc ctatgtggtg aggaactcag atcagttctt ttaatttact aacgaccaca
     4981 tccacttcct cttttgtatt atgcttacaa aaagaaaaac ggaccggaac caggttagga
     5041 tcattgctga tagccctgat aacatggctg cccacatctg ctccgctgct gcaggcactg
     5101 ccacccgata cacagatacc ctgcatatca aggttgaata agatcatttc agatttctct
     5161 gttttgggga atgaagcatt taatacagta tataaacttt tgcctgcgta atcaccgtta
     5221 aaccggacac ctttaatatg ctttctcaat tgctcaatca tataggtctt caatgattgt
     5281 attgatgcac tctccttttc catatgggta tctgctattt ccaatgcttt ggcaaaaccc
     5341 acaattccat acagattctc ggttcctgcc cgcatattac gttcctgccc gccaccaaaa
     5401 atcataggct ttactttgat attatcattg atatacaaaa tacctactcc cttcggccca
     5461 tgaaatttat gtcctgcacc ggctgcgaaa tgtaccggct tgctgcgcag gtcaatttta
     5521 taatgtccta ttgtttgcac tgtatcggaa tgaaagatag cattatactt tttacagatc
     5581 tcccccaccg catcaatatc cagcaggttt ccgatctcat tgttagcatg catcaatgaa
     5641 acaagacaac gttcttcccg actggccagt tggttttcaa ggtcgccaat atcgatatga
     5701 ccatcaggca acacttttac aaagctgctg gttaccacat catcatgatc gaaatgttcg
     5761 acagcatgca acacagcatg atgctcaacc ggtgaagtga tgatgtgacg gcagccaagg
     5821 tcatggatag cactggcgat ggccatatta tcactttcag ttccaccact ggtgaagaat
     5881 atctcgccgg gatttgcatt caggatcttt gcaaccgttt tacgggcatt ctcgatcgcc
     5941 agcctgcttt cccttccata cgaatagatg gaagatggat tcccgaaatg agtggtcata
     6001 tagggcaaca tcgcttcaag cacttctttg tcaagtgcag tggtagcagc attatcgaaa
     6061 taaatacggt tcataaatta tcttccgccg gtttaaaaaa ttgttggcaa aggtaagcaa
     6121 taactgcggt gaattaccgg gtatcctgct caggaaaaca gtaacgagaa aagatatttt
     6181 aacaccaata aataattttt gagccattct ttaatgaagt aacttggtag atattaattt
     6241 ctatggaaca aaagcaagac cattgttgta acggcgggag ccggcgcaac tttatcaaaa
     6301 aaacaactgc actaaccgct ttaagtattg tgccttcggc catcattaaa gcaggcgagc
     6361 accacgaagc cattgcttca tatatggaga aaactccggt acaactggag atcaatggaa
     6421 aaccatacaa agtgttggtc gacccagggg tcagcctgct ggatatgctc cgggaaaacg
     6481 taggacttac cggtaccaag aaaggatgtg accagggaca ttgcggtgcc tgtattgtac
     6541 atgtagatgg caaaagagtg aattcctgtt tgtcagaggc agtagacaac aatggtaaga
     6601 aaataacgac cattgaagga ctggcagagg gtgataaact gcacccgatg caggaagctt
     6661 ttataaagca tgatgctttc cagtgcggtt attgtacacc cggccagatc atgtcgggag
     6721 tggcctgcat cagggaagga caggcaggct cacccgatga aataaaagaa ttcatgagtg
     6781 ataatatttg ccgctgtggt gcttatccaa atattgtagc ggctatcacg gaagtaaaaa
     6841 acagcggaca gaaaatctga tcattgccgt tttaaccctt tttcctaacc agtatttaca
     6901 actgttatga acaacaagat aaaatttttc caaacagata atgatgacga ccgtgttgat
     6961 ggtattgata aagtaaccgg tcgtgcaaaa tatacagctg atcatatgct ggaaaatatg
     7021 gcttacgctg tatttgtaac aagcaccgtt gctaaaggcg ctattaaatc catgctcctg
     7081 catgaagcca ggaatgcacc gggtgtactc gatattattt actatcagaa ctgtccgcct
     7141 gttccgggtt accgccctta tgcaaaagac ccggcaaaga aaggatttga atggagaggc
     7201 ctgaaagtgt ttgataataa cctggtgtat tattatggtc agcccattgc attggtggtg
     7261 gccaccacct gggagaaagc agtaaaggca gcttcattgg taaaagcaga atatataaca
     7321 gaagcttttg aaacagattt tgaaacactc cggaaaaacg aaaaaaaatt aaagccggcc
     7381 ggccaatata aaagaggcga agaggatgcc tggaaaaatg cggaagtttc cattgaagca
     7441 gaatacttca ctcctattga aacccacaac ccgattgaac tgcatgctac gattgcagtt
     7501 tgggagggtg atgataaact gaccgtttac gataaatcac agggaccgaa aggaacacaa
     7561 tccgaactgg caagaaactt tggattggat gaaaaaaatg taagggtgat caccaaattt
     7621 gttggtggcg cattcggcag tgctttgcga agctggccac atgtacccgc agcctgtatt
     7681 gcagcaaaag tggtgaagcg tccggtgaaa ctggtactta acaggagaca aatgttcacc
     7741 atggtgggct atcgtccgca gtcatggcag aaaatttcaa ttggggcaac taaagaagga
     7801 aagttaaccg gtatcaccca tcatgcgatc agcaatacat cccgttatga agatttcagg
     7861 gaaggaattg taaacgcagc acaattttta tatgagtgcc cgaatgtaaa taccagctac
     7921 aaaatattac cgcttgatat caacactcca acctggatga gagggccggg tgaagctacc
     7981 ggtgcttttg cactggaatg tgccctggat gaactggctt ataaattaaa tatggacccg
     8041 gttgagttac gaattaaaaa ttatgcagaa gttcacccgg tgagtaaact accctggaca
     8101 agtaaattcc tgaaagaatg ctatgcagcc gggaaagaaa tgatcggttg gcacaaacgg
     8161 ccatcaatcc cccgttcact gcaagagaac ggaatgctgg ttggttatgg catggctggt
     8221 ggtgttttcg gtgccggcag agggaatgcc actgttaaag ccatacttaa aaatgatgga
     8281 acattgattc tgcaaagtgc tgttagtgat atgggaccgg gaacggccac tacgatggtc
     8341 aaagtagcag ctgaagtaat gcaaatgcct gccagtaaaa ttaagttcca gttgggtgat
     8401 acggattttc cacccggacc tacacagggc ggctccacta ccacttctac attgggttca
     8461 ggtgtaagca ttgcatgtga agcactgaaa caggcattaa aagaactcgc aagaacgatc
     8521 ataccctctt tttcaacaat tgctgcggaa gagttgggaa tagcagatgg atatattgta
     8581 tcaaaaaaag acagccagca aaaaatttcc ttcacagaac ttttgcagaa agcaggcaaa
     8641 cccgaaatcg aagtcataaa agaatcgacc cgggctacag cagagttcca gaaatacgca
     8701 atgaattctt tctctgcgca ttttgtaaaa gtgcatgtgc atccaaccac aggagctatc
     8761 gcactcaaac aaatcatcgc tacaggagac ggaggaaaga tcatcagcga aaaaacagcc
     8821 cgcagccaga tgatcggcgg tgccgttggc ggcattggta tggcattaac ggaagaagca
     8881 ctgatcgatc ataccaccgg taagtatatc acagacacat tagggaagta ccaggtgccc
     8941 aggcacagag atgtaccttt tatcgaaact tattttccaa acaaacctga tccgattatc
     9001 aacccgatgg gctcaaaagg ggtgggagaa attgccctgg taggttttgc acctgctgtg
     9061 gcaaatgcag tatttaatgc aacaggaaaa agagtgagaa gcctaccaat taagccagaa
     9121 catttgatct gattaaatct taccagttac gattttttta caaaagttgg tttcaactgt
     9181 tgacagggcc tatcatttat tttataagcg atagttccca aactaattta ctgcaaaatc
     9241 tcctatctct tgaagaattt catcaactaa actctttaat gtcacgcatc agttgcatcg
     9301 caatattatt ggcctttgtt tcaaaatcac tttccgcata aatgcggata ataggttccg
     9361 tattggaagt acgcaggtgt acccagtcat tatcaaaacc gatctttagt ccatcttcca
     9421 tattaatggg gttgttctta tattttgttt tgatcttctc aaaaatggct tttacatcca
     9481 ccccattgtg cagttcaatc ttattctttg aaataaaata atcgggatag gtagtgcgta
     9541 gcgatttaat ccctttttta tgatcagcca ggtggctcag gaataaaccg attccgatca
     9601 gggcatcacg gccataatga aaatcaggaa cgataatacc accgttgccc tcccctccta
     9661 ttaccgcatt agtctctttc atctttttca ccacattcac ctctcccact gctgaaggga
     9721 aatactctcc gccatgtttg agggtgatct ctttcaacgc cttggtactg ctcatattac
     9781 ttaccgtatt cccttttctt ttactcaaca cataatctgc cactgccacc aaagtatatt
     9841 cttcaccaaa taaagttccg tcttcattca caaaacataa acggtccaca tccggatcga
     9901 ctgctattcc cagatgagct ttctgctgaa tcacttcatt gcataattga gaaaggtgtt
     9961 ccggcaatgg ctcggggtta tgtgcaaatt ttccattcac ttcaccgttc agcacaatca
    10021 cattggttac acccaatgcc ttcaataaag ccggtacata ggtagcgccg gttgagttaa
    10081 tagcatccac tactacttta aaatcctgtt tggcaatagc tttcgcatta accagcgggt
    10141 atttcagaat agcatcaata tgtttttgta aatacgtatc atcggcagtt accgttccaa
    10201 gtttatcaac agttgcaaaa gaaaaatctt ccctggctgc gagttcgagt accctttcac
    10261 ccacttcagc agagataaat tctccttccc cattcagtag tttcaatgca ttccattctt
    10321 ttgggttgtg gctggcagtt agtatgattc caccatcagc attttccatt ttcactgcta
    10381 cttccaccgt aggtgtggtg gacagtccaa gatcaatcac atcaatacca aggcctgtca
    10441 gtgtattcac caccaggttt tgtaccatgg caccgctgat acggccatcc cggccaataa
    10501 cgatctttgc agattgtttt ccggttgcta tgatagttcc aaatgctgca gtaaatttaa
    10561 ccacatctaa aggggtaagg ttctcaccgg gtttcccgcc aatcgttcct cttataccgg
    10621 atatactctt tattaatgcc acaattttgt ttttaggtcg gcaaaaatac gttgtgcaaa
    10681 ttcaaccccc ttttaaaagt gcatccgtac ttttgttgga tggcagaaaa ggcatggtat
    10741 aaagaatggt tcaattcttc cttttatcac aaactctact ttgaaaggga tgaaaaagaa
    10801 gcggaagctt tcataaaaaa gctggtgcag cacttacatc cggctcccgg aagccgtatg
    10861 ctggatgttg cctgtggcaa aggcaggcat agcaagacgc ttgcttcact ggggttcaca
    10921 gttacaggta ttgatatttc tggtgacagt atcgcttatg ccaggcaatt tgaaaaagac
    10981 aaccttgact tttttgtaca tgatatgcgg ctgccgtttt gggggaatta tttccattat
    11041 gcatttaatt tttttaccag ctttggttat ttcaaaacca ggagggaaca tgataatgcg
    11101 atacgtacca ttgccagaag cctgaaaccc ggcggccaat ttgtaattga ttacctcaat
    11161 gtccattatg cagaagaaca ccttatttac aatgagtcga aagtaataag cggaacaaac
    11221 tatgctattc accgctggga ggatgaaaca catttcaata aaaaaataac agtaactgat
    11281 gctgctttaa aacagccatt ggagtatacc gaaaaagtag ccaaattctc cttaggtgat
    11341 tttacagata tgcttagtta ccagggcatg caggtacagg aggtttttgg ggattatcaa
    11401 ttcaacagct atgatgtcag aaaaacgcca aggctgattg taattgctga aaaaaagtct
    11461 aatcattctg attattcatc atcaaatact ttgacgtctc ataaaaggca gtggtgaata
    11521 cagagagctt ctttctgaca gaatcttttt tcgctgtggt ataattcaaa acgccggaat
    11581 gcaatgcatt caattgctct tcccctgaat tgatatttcg ggtaaaataa aaatcatccg
    11641 tcaccattcc tatcccatct gctgtattag taataaaagc aaaattattt ttcttaccgg
    11701 gatccagtaa atccctgccc aatgaggtat tgacataaga ctggtgcagc attcctgcaa
    11761 tagtcggcag tacatcgatc tgggaaacga cttcactcct tctttgagga accagtaggt
    11821 caggtgcata aaacaataac ggcacatgtt catcggttag acgttgttca gtccaaacag
    11881 gcgggtacat tgcctccgca ttacctgcca ggccatgatc tcctacaaaa acaaaaatcg
    11941 tattatgaaa ataattttct ttccgggctg cttccataaa catgcggaaa caataatctg
    12001 aatacctgaa gcagttgtat tcatccaaag attcaaagcc gtatttgatt aattcctctt
    12061 ccggcacaac aactcttaca aagtctgtat cactctctgg tattgtttta ttatagggcc
    12121 ggtgattacc cgatgtctgt atgtacgcaa agaagggttt atcttttttc cggaacaact
    12181 cgtttgcctc cagaaaaaga tctttatcac ttatacccca cacatttact ttaggcgctt
    12241 taaaataatc tccggtatgc atttgcaacc cgtcaatatt ttttaacagc ccttcaaaat
    12301 tgttgaactc cggacttcct cccaggaaat aatgtttttc atagtcctcc aggttatcta
    12361 caatagtatg ctgctgaaca gcctgcggat tccggctgga aaatttgaac aactgggcat
    12421 ccgggattcc ggacaggatc gcaaacaatg cccgggcagt agaaaaatgt ggtgaaaaac
    12481 aacgctcaaa aaatattccc tgccggctta gtgaatcaaa aaaaggagtt gcattcaatg
    12541 gattaccact catggtactt ttatacatgc tgaatgattc gcattgcacc agcacaatat
    12601 ttggcctgct ttcaaatgag gaactgcggg ggccaatttc cctgcgaaaa gaaaaatccg
    12661 atttatccgg caactccatc cactctgcca ttacaggaaa aacctccctg gcttttcttt
    12721 cgttgtaatc aggttttctc agtttaaggg ttgcaacaaa gttctgcagc gggttaatgg
    12781 ccaggtatga tttaaaacta ttgttgtagc ggaaactgtc tgaacggctt aacggtggcc
    12841 aggatagaga accataaata aaaaaacaca gcaagatcac tgttattatg aaaaattttc
    12901 tgcggtgtgt aatgcccaaa ccttctgtcc ggttgatcac ctgccagtgg cttcggtggt
    12961 acatccagcg gaagaataat acggcaacca ataatcccag cagcatccat atcaacggat
    13021 acgtttgcca tatcatcttt aatgaaatgc tgaaatcttc tgcaaaattc attgcccctg
    13081 catccagcgg tgtccggtta taagaaaaac ttccaaaccc tgctgcaaaa aagaaaaaaa
    13141 caatgaaggt tacaatagcg agataccatg tccaccattt tttgttgcgt gatgaataaa
    13201 atggggacaa acggggaaaa gtactaaata atacaatcgg cataagtatg atcgcaatcc
    13261 atcgcagatc ataccggaaa cccatcaaaa aagagggaac cacatcacca aaagaaattc
    13321 cttttggttt gaatgcaaaa aaagtagcca gcctgaataa tgtaaaaatc agcaggaaga
    13381 tgacaaaaag atttgccacc cataaaatgg tttttggtat tttgtatttt accagcatca
    13441 gcttctgccc ttcaaatgta ataatggcga taatgaagga atacaaagaa aataattaaa
    13501 aaggggatag caataccgga tatcatttat ttactatcgc tttaaagtat caatcccctg
    13561 caagccctac ttttgcaaca atgcctttct tagcaataaa catttgggaa aaactggtac
    13621 tatgggacaa atggctattt attcagataa acagccaatg gaccaatccg cttttcgatc
    13681 aaatcatgcc gctgtggcgc agcccggttt tatgggcccc gctatacctt tttttgctgg
    13741 tatttgtatt gatgaacttt aaagtaaaag gactttggtg gtctcttctg ttcatttgtt
    13801 cgattgccct gacagatatg gtgggcaatt atgtatttaa acatggaatt gaaagaattc
    13861 gtccctgtaa tgaccctgat ttttatatgc atgtcaggct cttgctggaa cattgcggag
    13921 tgggctatag ttttatttcc aatcatgccg ccaatcattt tggcatggct gtttttggtt
    13981 ttatcacctt acgacatata ataggcaatt gggcatggat cggaattgcc tgggcactgt
    14041 ctgtcgccta tgctcaagtg tatgttggcg tacattatcc gcttgatgta gccggtggcg
    14101 cccttctcgg ggtcatattc ggtgtaacta caggcacgat attcaataag cggttcagat
    14161 ttgctatctt cgataatcaa tcaattccgg cttcttgatg gatatattta tattaggcgt
    14221 tcttatttta gtgaatggcc ttttctccat gtcggagata gccctggtct ccgcccggaa
    14281 agcacggctg gagcacctgg cagagaaagg cgataagaaa gctaagctgg cattagaact
    14341 ttccaaccac cctgaattat ttttatctgc tgtacagatc ggaattacgt tgatctccat
    14401 tgtaaccggt gtgtactccg gtgaaaggtt tagtaagaac ctgcaacctg tatttgagaa
    14461 tattcccttg ttgcagcctt acgctgaaac tatctccacc acactcatca tcattctggt
    14521 tactttcctg tctatcattt ttggtgaact gatccctaaa agattcggat tgctgcggtc
    14581 ggagaaaatt tcaaaagcgg tggcaggacc catgaaaact tttggaacca tcacccaccc
    14641 tattgtttgg ttactgaaca ggaccagtaa tatctttttc accctcttta atatcaaacg
    14701 ttccaaagag gatgctgtaa cagaggaaga aatcaaaacg cttatcactg aaggggcgga
    14761 agcaggcact attgacgaag cagaacagga aattattgaa agggtctttc acctgggcga
    14821 ccggaatatt acttccctca tgacacaccg gagtgatatt atctggttta acctggatga
    14881 taacgaagat aagatcaaag agaaaatttt acaggaaaca cattcagcat atcctatctg
    14941 cgatggatca attgataata ttaaaggagt ggtatctatt aaagatctgt atataagccc
    15001 ggataacacc ttattcaagg agatcatgca accggcctta ttcattcctg aaaacaattc
    15061 gccgtaccag gtaatggaaa aattcaaaga atcaaaaata cattccgcct ttatcgtaga
    15121 tgaatatgga agtatactag gattattaac cctgaatgat atcctggaag ccattgtggg
    15181 tgatattccg caacagaatg tacccgatta tgaaataatt gaacgggaag acggcactta
    15241 tatagttgac ggacagatcc ctttttacga ttttcttacc cggtttgaaa aaacagaatg
    15301 gatgaatgaa ggagaacatg atttcgatac attggcaggt ttcatccttc atgaactgga
    15361 acgaataccc aaaacaggtg acaaactaaa ctggaagggt tttgctattg agatcatcga
    15421 tatggacggt catcgtatag ataaagtgct cgtcaccatc agcaaggaac tcagagaaga
    15481 aatggaagag taattttttt caaaaaataa atacagcccc tgcatccggg aaattcaatt
    15541 tgctgctttt tctgtttcca ttcactgtta catgcataat attcatctgg tcatcaaact
    15601 gatcatacat aatatccgta gtcacttcca gtttatttac caccggtaca ttttgcactt
    15661 cgatataact gtatgcagct tctttatcat tctcaaaacc gagataggaa aataaaacag
    15721 gtttgccatt tacttttatg gataaatgac tgaacaggta ttttttgacc agtgagtcca
    15781 ttgcagcctt attgggagga ttgataagat caacttttgc tttgtaattc ttagccagta
    15841 tcttctcaaa atcatcggta aagatcttac aactgatctc taatgttttg tcaacagcat
    15901 tatgattgat ctctatgaca ctgacatgaa aagggtgcac tatcaccgga cgggccgcca
    15961 tcaaaataat agctaccagc ggaataatcg accatttata gacggatgct gccattgaca
    16021 caatattttt catagttaca caacttgggc ttattttgaa gcctgttaca gcttgagacg
    16081 ctaaaaatag cgtaatggtt cgaacattga tgagaaaaaa gtatgagtga tttcaatttc
    16141 tattttggtt taggctggga gcatattatg agctgggatg cactggatca tttgcttttt
    16201 atcgctgcac ttgctgccat ctatttatta aaagactgga aacaggtcct gatattggtc
    16261 accgcattta ccctcggcca ttcccttacg ctggcactaa gtgtgatgga tgtttttcgt
    16321 ttcccttcca actgggtaga attcctgata ccctgcacta ttgtggtcac tgccatcagc
    16381 aacctgtttc agaaaaaatt tacaacaaag tctatccgca tcaattattt cctggcatta
    16441 ttttttggtt tgatacacgg catggggttt gccaacacca tccggttcat gctggcaaat
    16501 gaccaaagtt ttggatgggg attgtttgga tttaatgtag gcctggaggc cgggcagatc
    16561 gttgttgtca gtactttgtt attagtaact caggtactta tcaatatagc aaagattaac
    16621 cgtcgtgaat gggtcatctt tttatctgct gctatcttta gcctggccct gaaaattgca
    16681 atagaacgtt tacctatata ataataattt aagccatgaa gaaaatttgg atattatgct
    16741 gcctcctgct gccatttaca atagcgacaa aggcacaaat tcagaataat gccagctcca
    16801 accatgccaa caagtttgag caattgggga caattttacc aactcccaat gaacagcgta
    16861 cagccagcgg ggcacccggc accaaatact ggcagcaacg ggtggattat gatataaaat
    16921 gtgaactgga tgaagtcaat aataaattga ctggcagcga aacgatcact tactttaata
    16981 actcaccgga tgtattaaat tatttctgga tgcagctgga cgaaaaccag cacagcacca
    17041 ccaaaaatgc cgattatcaa agcagtaacc gcctgcctaa ccagacggtt gacaaaatgc
    17101 ttgaccggtt tgaagaaaaa aggtcggaca atggttatgg tgttaatatc acaaaactta
    17161 ttgatgccgc cggtaaacca ttaaagtata ccattaataa aacaatgatg aaggtggacc
    17221 tgccggctcc attaaaaccg ggacagaaat ttgtttttaa agtagactgg aattacaaac
    17281 tggccaaccg gggtgatttt atgcgtttcg gcggagcaag aggcgggtat gaacattttg
    17341 cagaggatgg taataataat tataccatga cacaatggta tccccgtcta tgcaagtaca
    17401 gtgatgacca gggctggcaa aaccaccagt tcaccggcac gggtgaattc acattatgtt
    17461 ttggaaattt caaagtacag atgacagtgc ctgccgatca tattgtgggt gccaccggtg
    17521 aatgccagaa ttatgcacaa acattaacgg caacacagat ggcccgttgg caaaaagcac
    17581 aaacagccaa agaaccattg cagatcgtta cattagaaga agcattagcg gcttctaaga
    17641 aaacaagtaa cagtgctaaa aaaacatgga tctataaggc tgataatgtc cgtgactttg
    17701 catggacatc atcccgaagg tttgtatggg atgccatggc caccaatgtg gaaggtaaaa
    17761 aagtaatgtc gatgagctac tatgccaaag aagcctaccc gatctacagt aagttttcaa
    17821 ccaaagcagt agcacatact ttaaaaacat attctaaatt ttctttcccc tatccctacc
    17881 cggttgccat cagtgtagaa ggtaaccagg gtatggaata cccgatgatc tcgttcaatc
    17941 ccggccgcgc agaaaaagac ggcacgtata cagaaggcgc caagaatgca gcaattcttg
    18001 ttatcattca tgaagtaggg cacactttct tcccgatgat cgttaacagt gacgaacgcc
    18061 aatggacctg gatggatgaa ggattaaatt cttacttaca atatctcaca caggaattat
    18121 gggataataa attcccatcc gatggcggac cagcctggac cattaccaac tacatgaagc
    18181 tgccaaagga ccagttagaa cctattatga ccaattcaga aaacatcaac aacttcgggg
    18241 caaatgcata cgataaagtg gcaacaggat taaatattct cagagaaacc attgttggcc
    18301 gtgaattatt tgacaatgcc ttccgtgaat atgcaagacg ctgggccttt aaatcaccta
    18361 cacccgctga ttttttccgc actattgaag atgccaccgg tgaagacctt gactggttct
    18421 ggagaggttg gttctatggt actgatgcct gtgatattgc tatcgacacg gttaaacatg
    18481 ccgtgccgga tgtaacagca gtgccttcac aaacgaaaga taccactgta atgagaggtt
    18541 tacccaaacc ggatgtcaat ccatttgaag atatctccaa gatcaggaat aagcaggaca
    18601 agaacatagt gtttgaaact gataaggata ccagccttcg tgatttttac tggaagtatg
    18661 acagggaaat tgcaaaatat gatactgcaa aatatcctgt tgtagtaaga gcacagacgg
    18721 atgcattgga tgaagaaggc cgtcagaaat atggcagcaa acacttatat gaaatttcct
    18781 ttaccaataa aggtggattg gtaatgccga tcattctgga atggacatac aaagacggta
    18841 caaaagaagt agaaagaata ccggcacagg tttggagaaa gaatgaaaag aatgtgatca
    18901 agacctttat gaaagataaa gaagtagctt ccattaaact ggatcccttc agggaaacag
    18961 cagatattga tgaaagtaac aatacgtacg gcaatattaa agaaccgggt aaattcaaat
    19021 tgttcaagca aaaacagaat gtgacgcctg ccccgggcat caacccgatg cagaaagcac
    19081 aggaaaagaa aggcttctaa ccccctttat atctcaaccg aagggaactt ccttacataa
    19141 ttatcaaaaa gccgctcata aaaaaagcgg ctttttattt tgccttactg cttaagggga
    19201 aatgcgattt ccttagtttt ggtgaaacgg ctgaaataaa tatctattat ttaaaaaatc
    19261 aatataccac atgagacgtt ttatcacctg tttattcttg gcattggggt taagtgtaac
    19321 aagttttgca cagaatatat ataacaaccc gaacagcaat catggtaata aatttgaaca
    19381 gatgggtggc accttaccca ctccgaatga gtaccgcact gccagtggcg ccccgggacc
    19441 ccgatactgg cagcaaagag tagactatga tatcaaatgt gaactggatg agaaaaacct
    19501 gaaacttaac ggtagcgaaa ccattaccta tttcaacaac tctccgaatg aactggtatt
    19561 tctctggttg caattagacg agaaccagca cagcagtgtg aataacgcca attaccagac
    19621 cagcaataca atgagcagac aaacaagtcc gcaacagctg gacaggattg acgaagctaa
    19681 gactgataat ggctatggat ttaatatcat gaaacttaca gatgccgccg gtaagccttt
    19741 aaaatttact atcaacaaaa ccatgatgcg ggttgaatta cctgcgccac tgaaagccgg
    19801 gcagcgtttt attttcaaac tggattggaa ttataaactg gcagaccgta tgggtcgtgg
    19861 tggccgtggt ggttatgaat actttcctga ggatggcaat catttattca cgatgactca
    19921 atggtatccc cgcctttgtg tgtacagcga tttccaggga tggcagaatc accagtttac
    19981 aggccgtggt gaatttgctt taacctttgg caatttcaaa gtgcaaatga cagtaccggc
    20041 ggatcatgtg atcatgtcaa ccggtgagtg ccagaattat cctgctgtac tatcagctgc
    20101 acaactggcc cgctggaata aagcaaagaa tgccaccgaa ccaatggaag tggtaacgct
    20161 tgatgaagcc aaagctgctg aaaaacaaaa aagccaaaat aaaaaaacat ggattttcaa
    20221 agcagataat gtccgtgatt ttgcatgggg ttcttcccgt aaatttatct gggatgctat
    20281 ggcaaccaaa gttgaaggta aaaagatcat gtgcatgagc gggtatccta aagaagccta
    20341 tggtctgtat agaaaattct ctactaaagc tgttgaacac actataaaga catattccaa
    20401 attcactatc ccttacccct accctgttgc acaaagtatc gaagcagcca atggtatgga
    20461 gtacccgatg atctgcttca attttggccg taccgaaaaa gatggtacct atagtgaagg
    20521 caccaagaat ggtatgctgg gtgtgatcat tcatgaagtg gggcataact tcttccccat
    20581 gatcatcaat agtgatgaac gtcagtggag ctggatggat gagggactga atactttcgt
    20641 ggaatacctg gcagaagaat tatgggataa taaatttccc agccgccgcg gccctgctgc
    20701 ctttatcaca gattacatga aattaccaaa ggatcaactg gagccgatca tgaccaatag
    20761 tgagaacatc gtacaatttg gccccaatgc ttattccaaa ccagctacag gtttgaatat
    20821 tctccgcgaa actattatgg gccgtgagct atttgattat gcattcaggg aatatgcaag
    20881 aagatgggct ttcaaacatc ccgagccggc tgatttcttc cgcacgatgg aagatgccag
    20941 tggtgaagac ctggattggt tctggagagg ctggttcttc agtactgaag tgtgtgatat
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atacgaagtg aagatcccgg
    21121 gtagtaaacc tgaagggtta aatgatgcag aaaaagaaaa gtatggcaac aaaaatatgt
    21181 atgaactaac cttcagcaat aaaggtggtc tggtaatgcc gataattctg gaatggacct
    21241 ataaagatgg caccaaagaa gtggaaagga taccggtgca ggtgtggcgt ttgaatgaga
    21301 acaaagtgat caagactttt gtgaaagaca aagaagtagc ttctatcaaa ctggacccga
    21361 tgagagagac agcagatatc aatgaaagca ataataactg gaacacaata cccgaaccat
    21421 ccaggttcac catctttaaa caaacacagg gcggcggcag aagaggcggc ggcggacaag
    21481 gtggcaaccc tatgcaaaaa gcactggaga ataagaaagg attctaaaga agtgtaaagt
    21541 ttatagttag gaggcatgat aaattcatgc ctcctctttt tatatctgca tatacccgcg
    21601 gagttatcgg cgcaatctgc gggaaaaaat caggctttac ttttaacttt accggatgaa
    21661 aagaatttcc tgtttattat ttaccctgct gctctcttat tgtataaagg ctgctgctgt
    21721 ggatacaata actatttaca gcactgccat gcaaaaggaa tataaatgtg tggtgataca
    21781 accaacagcg atgccggata aaaatacacc actcccggtt gtttatttat tacacggaca
    21841 tggtggctgg tatgccaact ggatcataag agttccgcaa ctgaaagact atgctgatca
    21901 atatcaatta atgattgtct gcccggatgg cggttataac agctggtacc ttgacagtcc
    21961 attggattcc acggtacggt ttgaaacgta tgtcagtaaa gaaataccag cttatattga
    22021 tgcacattac aacactatca aagacagaaa agcaagagcg attacagggt tgagtatggg
    22081 cgggcatggc ggattgtttt taggtttcag aaatgcagaa cgcttcggtg cctgcggcag
    22141 catgagtggt ggtgtggacc tgcaaccctt tccgaagaac tggcaactgg cacaaaagat
    22201 cggcgataca ctgaattatg cagcaaactg gaaaaactat tccgtgatca atgtgatcga
    22261 gaactatcct gaagattctg ttgcaataat tattgattgc ggcaatgaag atttttttat
    22321 caacgtaaac catgccttac atgaaaagat gctgaagctg aagataccgc atgattacat
    22381 tgaacggcct ggtaagcatg actggaatta ctggagaaat gcagtgcagt accagttatt
    22441 gtttttcagg aattattttg agaatagccg aaaatgatac actaaggctt cggtaagttg
    22501 ccggtactac catcccttac ccgtattttt ttcttaccgg aatttttctt ttcgtaatcc
    22561 aggatggctt gtggtttggt gacagttttt ttacccgccg tatcagtctt tgttttacca
    22621 tcacttacac ttaaaggctt aatattttca tcatccagca tcccgccatc ttcggtttgc
    22681 agtttattca tcacataatt ctccgtggct tcaatggtac cttctctcgg atcgtagtat
    22741 atccatttac catgtttcat ggctacacct tcattcttca ctaccaccct gtctatttcc
    22801 ttatcggtgt ctctcagatc gtaaaccgca accgtatcaa atttattggc cggatccatg
    22861 gccctccagt tttctatccg caacaacccg ccattataag taaagtattg ttgccttccg
    22921 ttgagcttgc cccagcgata attctcttcg gcgatcttca ctccttccag ggaatacttt
    22981 ttccaggtac cttctttctg gtcattctca taatagccct ctgcttcata acccctttct
    23041 ccccgcaggt catctacacg aacagaccag ggaccctgtt tgcggccatc catatctacc
    23101 cggttcagcg tgtcgccatg tttgttcagg gtaaagcttt tccattgtcc aaaactattg
    23161 atggaaataa ataataaaag gaggggaatc aatcgcatag tttagtaagt ttgaaagaga
    23221 agctataagc tttcagcggt acgccgggag ccgttagcca ctgcaattta ctaacgaaat
    23281 ataactgctt ttatgtctgt cttgttaaaa aaaatgggat ataacacccg gatatttctt
    23341 ccttttgttg ttattcttgt caatttacac cttgccacat cagttttcgc acaggttccg
    23401 cctaccccgg ctgctgaaag attaaaagta acagaacaac ggaaagccct ggaatccaaa
    23461 tcagtattga atgaaataaa atttcgcaat atcggtccga ccatcatgag cggccgtgta
    23521 gtggatattg atgccaatcc cgaagaccca acagaattct atgtggccta tgccactggt
    23581 ggcctctggc atactaccaa caacggtcaa agctttacgc ctatcatgga taacctggat
    23641 ttgttattca ttggtgatat tgctgttaac tggacatcgg ctccaagaac gatctgggtc
    23701 ggcaccggtg aagtgaacag cagccgttct tcatatgccg gcatgggtat ttacagatct
    23761 gctgacaacg gaaaatcatg ggaatacctg ggcttaccgg aatcgcatca tatcggtaaa
    23821 atacaattgc atcctacaga taacaacatc gtgtgggttg cagcactggg tcatttatat
    23881 tccgccaaca aagaaagggg agtttataaa actactgatg gtggtaaaac gtggaagcag
    23941 accttgctgg tggatgataa taccggttgt gttgacctcg atattaatcc gtccaaccct
    24001 aaagaaatat atgctgcgat gtggtaccgg gtacgccgtg cctggaagtt tgaagaaagc
    24061 ggaagttcca gtggcattta taaaagcaat gatggcggag atacatggaa actggtttcg
    24121 ccagcgggtt ctggttttat gactggtgat aaaattggac gtattggaat tacagtttat
    24181 cctaaaaatc caaacatcgt gtacgcagtg gttgataata ataccccaaa accggctgtt
    24241 gttaataaaa atgattcaac gtataaaaaa gaggaattca agaatataac taaggaacga
    24301 tttgcgcaaa tgagcagtaa atggctggac acttttttaa gggccaaccg tttcccgaga
    24361 aaatataatg ctgctgttgt aaaagaattg gtcgctgctg ataaaataaa gcccacagcc
    24421 ttatgggatt tcctcgacag tgatgatggt tttcaaaaca ccggtattgc cggttgcgaa
    24481 gtatatcgca gtgatgatgc cgggctaagc tggaaaaaaa caaatgaaaa acccattggc
    24541 atattcaata cctatggtta ttacttcgct aagatctata catcacatta taatcctgat
    24601 aaggttttca ttctcggttt ctatgcagaa gtttctaccg atggcggtaa aacatttaaa
    24661 acaatggata agggaaatgt acatgccgat caccatgctt tgtgggtaaa ccccaaaaga
    24721 gattcacatg tgatcaatgg aaatgacggc ggtgccaata ttacttatga tgacggagca
    24781 aactggttca aagcaaacac tccaccattg ggacaatact atgcggttac tacagacgat
    24841 gccaaacctt ataatgtata tggtggtatg caggataacg gcagctggtg gggtccttcc
    24901 aatcacaaag aagatatcgg ctggatcgat aatggtcagt atgcctataa aatgatcaat
    24961 ggtggtgatg gaatgcaggc acaggtagac accagggata atgctaccgt gtattccggt
    25021 tcacagtttg gcgtatacgg acgctacaac aaggacagaa gaggcgcaca aaaatctatt
    25081 cggccgcaac atgaactggg ttacaaaccc ttacgcttca actggcaaac ccccatcctc
    25141 ttgagcaaac acaaccagga tattttgtat tatggaagca acaggttgta tcgctccctg
    25201 aataaggggg atacaatgat cgccatgagt aatgacctga ccaatggaag agtaagcggt
    25261 aatgtccctt atggcacttt aaccacaatt tcggaatccc cgcttcgctt cggattgatc
    25321 tatacaggaa ctgatgacgg taatattcat cgttcttttg atgggggtta cagctgggaa
    25381 caactgaatc aaacaaccac tgcagcacct gtaaaaaaag gaacaaaacc ccctgccacc
    25441 aacactaaac tgaatactaa ccaactctgg gtcagtcgtg tcacagcgtc acaatataaa
    25501 gaagcaaaag tttatgcttc tttaaatggg tatcgctttg accattttgc tccttatctc
    25561 tatgtaagtg aagatcatgg taacagctgg aaaccgattg gtaaagactt acccaatgaa
    25621 cctattaacg tagtgaaaga agatccgaaa gatgagaaca ttttatatgt aggtacggat
    25681 ggtggtctgt atgtaagttt tgatgccggc aacagtttca tgttatggaa tggcggattg
    25741 cctaaatcag ttccggtgca tgatatcgcc attcagcaac gggacaatga actggtattg
    25801 ggtacacatg gccgcagttt gtatgtaact aaactggacg atgtacacgg tataaaaaaa
    25861 gatcccgatt ggctgaagaa aaagccgaag gaaaaaacac aggaaacaag aaggccggca
    25921 gataataatg aaagtgaccc ggaaatgtaa atacaaaaaa ggctgtctta aaagttggtt
    25981 tgattgttat attgtctcga tacctatacc tatcgggata gcgaaatatt aacaatcaaa
    26041 ccaatagcct tcggcaaatt caggccgata tagatttaaa aacagcctct ttattttata
    26101 caaagggcag gtaaatccta aaaaacttta aacctttcga aacaggcttt ctccagtatc
    26161 tccctgtcat cagggtgtgc aatctcaatt aacgcctttg cccgttggcg aagattcatt
    26221 ccaaaaaggt atgcaacgcc aaactctgtc accacataat gaatatgccc acgggtggtt
    26281 accacaccgg caccgggttt cagaaatggt acaatacggc tgacaccttt agtggttctg
    26341 ctggtcaggg caatgatagg tttccctcct tcactgagtg cggcgccccg cataaaatcc
    26401 atctgccctc caatcccact gtattgtttc gtgccgattg aatcagcaca tacctggccc
    26461 gtgatatcca cttcaattgc actgttaatg gccactactt tcggattgcg gcggataaca
    26521 tgcggatcat tgacataatc aatatccagg aaaacaaaag ccgggttatc atctacataa
    26581 tcatataatc gtcttgtgcc aacagcaaaa ccggtaaccg ttttattcgg gtgaatcctt
    26641 ttcttcgaat tattgatcac atcattatcg aatagatcaa tgataccatc actgcacatt
    26701 tctgtatgca cacccaggtt tttgtgactg tggagcgatc ttaatactgc atctggtatg
    26761 gttccgattc ccatctgcaa agtgcttcca tcttcgatca aaccggcgat cagttgcccg
    26821 atcttcaatt catcttcacg aaccttggta ccatagtcaa cctccggcaa atcttcttcg
    26881 tgatacacca ttgctgtaaa acgcttggtg tgtaccaggc tgtcaccgtg agtacggggt
    26941 acttttggat ttacctgtgc aataatatgt gtagctgtat ttacggcgct gcgggctaca
    27001 tccacagata ctcccagcga acaatagcca tgctggtcag gaggcgacac ctgtaccagc
    27061 gccacatcaa tttgcatttg tttgcggaaa aggtcaggga tatcactcaa aaaaacaggg
    27121 acaaagtcag ccctcccctc attcactgca ttccggactg attctgaaac aaacatgcaa
    27181 ttgatcttaa aggcatccgc atattgcggt ttgtccagtt ctatatcacc atgcacggta
    27241 atgaatgtta actcaacatc acgaagatca ggggctcttt tggccagttc tcttaataaa
    27301 taaagcggtg tctgggcact gccctggata aatactcgct ggccggattt gatgacggcc
    27361 aaagcttctc cagcagtcat atattcaaca ggtaatttca tactgcaaaa ctattgcaaa
    27421 taacagattg aaaaatgatt atccttattc agtaaaatga taagaatcag tagaattatt
    27481 gagcgatcag ccagtcaatg gctaattcgt accctttcaa ccccaaacca aaaatacttc
    27541 ccgctgcttt gccgctaaca tgtgataatt tccggaaatc ttcccttgca tgtacattgg
    27601 agatatgcac ttccaccacc ggggttttga tagcagcaat ggcatcaccg attgctacag
    27661 atgtatgagt atagccaccg ggattcaaaa caataccatc gtactcaaat ccaacccgct
    27721 gcagttcatt gatcagttct ccttccacat tgctctggaa ataactgatc tcaatactgc
    27781 catatttctt tttcaaagaa tcaaaaaact gttcgaatgg catattaccg tagatatcag
    27841 tttctctctt accgagcagg ttcaggttgg gaccgttgat gatagcgatt ttcatacatg
    27901 ctaaagtaga aaggaaaaag acaaaagtca aaagtaaaag atgaaaccac aaccaggatt
    27961 attttatcac tggaccctct cagcagccag caaactataa atcattaaac tgatgtgctt
    28021 accatctttg atctctgctt cccgcaaagt tccttcgtag gtaaagccca gtttctccga
    28081 caaacggcaa ctggcttcat tacctgcttc aataacggct tcgagccggt gtagtttcca
    28141 atgggaaaac aaatgagcga tcatcaccgg tatcacttca ggcataatac ccagcttcca
    28201 atgatccggc aacagccagt aaccgatctc tgctttttca tgttcgggct ggtaggcatt
    28261 catgccacag gcgccaacag gcttatcagt cgttttgctg acgatcttcc accagcaccc
    28321 ggtcttgtta cgccacagct catcataaaa ctccatctgg tttttggtgg cttcaaaact
    28381 atcatagcgg acaccataaa aagggatcac cttcgggtcg ctaagacctt tgaagataaa
    28441 tgcctgatcg gcaggaagaa tctgcttcag gatgaaacgg tcggttatta attcgggaaa
    28501 caagcgagat gatttgaaaa ttatacaatt gccggggaaa ctacaacaca gcgtaaccgg
    28561 gttcatgagt tacacagtaa ggctaactca gcgcccctct gtacctctgc caatctatgt
    28621 tgaaaaatcc taattctgct gcgacctgat cagcgcaata aactcatcaa ataaataccg
    28681 gctatcatgt ggcccgggcg ttgcttccgg gtgatactgc actgagaaag caggctttcc
    28741 ttttacccgg ataccttcaa tactctgatc attcaggttc acatgggtaa tctcaatatg
    28801 agaagctttc ttcaccgcct ccgggtcaac cccaaaacca tgattttgtg tcgtgatctc
    28861 tgaccggccg gagataatat tcttcaccgg gtggttcaat ccccggtgac cgtgatgcat
    28921 tttaaaggtg gg
//
LOCUS       DAORTI010000032        28571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1201_length_28571_cov_7.868951, whole genome shotgun sequence.
ACCESSION   DAORTI010000032 DAORTI010000000
VERSION     DAORTI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28571
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_28571_cov_7.868951"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLO70_06145"
     CDS             complement(<1..278)
                     /locus_tag="PLO70_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74075.1"
                     /translation="MGILKKSIIGISTMLLSLFVTAQEEAKLQAAFKESYTQESVRNF
                     TGAIATLAKLYDEKSYELNLRIGWLYYLNKDYPQSQSYYQKAVALRPYA"
     gene            complement(266..1444)
                     /locus_tag="PLO70_06150"
     CDS             complement(266..1444)
                     /locus_tag="PLO70_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74076.1"
                     /translation="MRKLIITTALAFFLAVLDTKAQQDFSFATVDSQSFALYEKADWK
                     ALLNYGKTAIVNNQDFLLLRLRLGYAAFMLTNYSEAIRHYEVVLKEDQYNNTAHYYIY
                     WSRINLNQPEIAMAEVKYLSKGAIEEKKSKPAALTSAALELSYKQTSVTTRKNPFYAR
                     VGLGNRFSHGFHMQQSVATYQQKINEPLLTAVTHNNNINIRQVEYYNRLMLNLDRHWQ
                     LKAAYHYLHTPFNNFKYNNHLLLVGFKYNGNYFDLQADAILGKLTDTSVQQYNLQVGL
                     YPKGNLDLYSFSTAMVRQQEGTAFNFKQVLGVKVLKNIWLEGNITLGDFHNLAENDAL
                     YVYNAIDPNKMKAGVTGIFLLPGNTVFQLGYTYEQRQLFGTTINFNQHSITGGLSWEF
                     "
     gene            complement(1432..>1878)
                     /locus_tag="PLO70_06155"
     CDS             complement(1432..>1878)
                     /locus_tag="PLO70_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74077.1"
                     /translation="YFYCRQQHAYAYFINNGTMFYFTNYFGNHHSLLYHFYKAAYKVV
                     LSTDKKVTITDTYPQNIFGRHPLKWIQDLLAPFYLFMKMKYTSHVKQDDNMMGGGTIV
                     LESRADQQLGNNLQQKMTARVVITNGKLQAFRVELSNKQIEAICGN"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1979..3423)
                     /locus_tag="PLO70_06160"
     CDS             complement(<1979..3423)
                     /locus_tag="PLO70_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HQZ74078.1"
                     /translation="MKRTTFILFLSYITTTVLNSYSMLFFSNKKYFASVVLLITFFNP
                     YTGLAGFIASLVAVLVAYFIGFSREQVKSGLYTYSAVLVGLGMGTFYEFSAGFWILLA
                     LAAVLSVLLSAAFIAKLGKSNLPALSLAFIFTLWVVILSSREFAVIGLTQRNIYWLNE
                     MYATGGTDLIRLVQWFENLSLPDVVTGYLRSLSAILFQSNIAAGIVLTIALLFFSRIA
                     LTLMIAGYAIAIVFIQLMDGYAGSVNYYNLGTNFMLVSVALGGFYIIPSVRSFFWSLI
                     TVPISYILVIALWKITYTWGLPVFSMPFCITVILFLYVLQLRRTGGKMVLTPIQYYSP
                     EENLYRYTNNKERVWNSFYYYHLSLPFMGEWTVSQGYDGSITHKGDWGKALDFVITDK
                     EKKTYQGSGSLPEQFYCYNKPVLSPADGIVEEIIDYVDDNPVGGNNVLQNWGNTIVIK
                     HAEGLYSKLSHLKKGSFKVAKGAYIKKGDIVA"
     gene            complement(3448..4806)
                     /locus_tag="PLO70_06165"
     CDS             complement(3448..4806)
                     /locus_tag="PLO70_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74079.1"
                     /translation="MKKLVYESPSIQKMNTGLMNKFGTRSEYEPVKQIDGVSVHTMMA
                     QYGSPLFVLSERTIRNNYQHAVRAFTTRYPKVQFAWSYKTNYLNSVCRIFHQEGSWAE
                     VVSGFEYRKALGNGVPGNKIIFNGPEKTNEELEMAVTNGSLIHIDHFDELFQLIELSD
                     TLKKKPRVAIRVNMDTGVYPMWDRFGFNYENGQALNALTKIVESEKMELTGLHCHIGT
                     FMLTTNAYATAVKKLGDLAWKCKTQLNTPIQYIDLGGGFPSSNTLRGSFLPGTDSVPS
                     VDEFAEAITTNLLGLGFSTEELPLLVLESGRVLIDDAGYLLSTVLANKRLSDGRRATI
                     LDAGVNLLFTSFWYDHKISPAQDFTQHTEDMALYGPLCMNIDVVRQSINLPLLNRGDK
                     VVVHKVGAYNMTQWMQFIQMRPNVVLIDEQGKTHVIREAETLQYMEQPEKVPEHLQTI
                     NI"
     gene            complement(4821..5873)
                     /locus_tag="PLO70_06170"
     CDS             complement(4821..5873)
                     /locus_tag="PLO70_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQZ74080.1"
                     /translation="MEKPDNLLTIAVTGLNAIDSPGPGIAVIRGLREGLHRPVRIIGL
                     SYETLEPGIYMHDVVDKTYQIPYPAAGSEALLRRLQYIHEKEQLQLIIPNFDAELHNF
                     IRLGPALKNMGIATFLPSQEQFDARDKINLYKFGEKHNLLVPKDKIINSPAELNACEE
                     ALGFPLVVKGKFYDAVVAYTLEQAQKAFYKLTAKWGFPVIVQEYINGTEINIAVLGDG
                     KGNAVSIIPMRKLYITDKGKAWAGITLEDDTLIKIASDFVKATQWKGGCELEIMRTKK
                     NELYIMEINPRFPAWIYLTAAAGQNQPAALAKMALGEEQPFYTAYEVGKIFIRYSWDH
                     ITDIKEFHQVSGMGEL"
     gene            complement(5890..6132)
                     /locus_tag="PLO70_06175"
     CDS             complement(5890..6132)
                     /locus_tag="PLO70_06175"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HQZ74081.1"
                     /translation="MYLKKNIATSESGFIFNPSTGDSYSANPVAAEIVQLLKQGTASS
                     SVKEQILEKYEVDPFRLEQDWDDFMNQLKEANLLGD"
     gene            complement(6237..>6744)
                     /locus_tag="PLO70_06180"
     CDS             complement(6237..>6744)
                     /locus_tag="PLO70_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74082.1"
                     /translation="GMTITIRNWKQEDLNNLVFHANNKNVWNNLRNYFPHPYTEEHGK
                     AWLEKVVDAVPAINMAIDVDGQMVGGIGLILNGDVYSKSAEIGYWIGEKYWGQGVATV
                     AVGQMTEYAFYYFDLVRLYAEVFENNKASMRVLEKNGYYLEGVRRKAVFKNEVLMDDY
                     IWVKLRSW"
     assembly_gap    6745..6844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6998..7540)
                     /locus_tag="PLO70_06185"
     CDS             complement(6998..7540)
                     /locus_tag="PLO70_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74083.1"
                     /translation="MMRKTQILTWAVVLLLVTNAVTIGTIVYHNYRESKKSDDVVIAG
                     SYGGNMINGRFLRQTLGFDQSQMSAFREINQAFRPVAMSLTFEIDSLKNAMFMEMQKN
                     QPDTLALNSMSAQIGELHGQLKHETYSFYLNTKKICSPEQRPELEKIFQPLFINEGIT
                     TAPNQHRGRGWEKNKTINNN"
     gene            complement(7550..7858)
                     /locus_tag="PLO70_06190"
     CDS             complement(7550..7858)
                     /locus_tag="PLO70_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74084.1"
                     /translation="MKQEKDIQQLLENFIGEERNMESNPFLSTRVMAAIAKNGIQNEK
                     RISPVWKTVMIGAGLVVAVFTGITIGSLYQSGNERPGVVLMNDNNMEHFGFYEQMVNE
                     "
     gene            complement(7902..8450)
                     /locus_tag="PLO70_06195"
     CDS             complement(7902..8450)
                     /locus_tag="PLO70_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ74085.1"
                     /translation="MQTEELIESILNGNHRDFEQLIKKYQPNVFRVAMGLLHSKEDAE
                     EITQDVFVKVYQSISAYNGKSAFSTWLYRITINTSLNFLKKKKRNRFWTGISQLLQVA
                     AKDKQAETLMDEKSDSAIIRQAIYALPEKQQLAFVLTKYEELPQKQVAEIMHTSEGAV
                     EQLVIRAKTNLRNKLEKELDRP"
     assembly_gap    8692..8791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9100..10758)
                     /gene="sulP"
                     /locus_tag="PLO70_06200"
     CDS             complement(9100..10758)
                     /gene="sulP"
                     /locus_tag="PLO70_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HQZ74086.1"
                     /translation="MFRPKIVDTLKSYSRQQFSRDLMAGLIVGIVALPLAIAFAIASG
                     VSPEKGLYTAVIAGFIISAMGGSRVQIGGPTGAFIVIVYGIVQVHGVNGLIIATFMAG
                     IMLIIMGFARLGSVIKFIPHPLIIGFTSGIALIIFSSQMKDFFGLQMGVVPADFLDKW
                     ISYVANFRTVNVYAVLLAVATVLIILFWPKITHKIPGSLIAILVTTAAMQWFNLPVET
                     IGSRFGSIPSSLPTPVVPDIDFATIKELIQPAFTIALLGGIESLLSAVVADGMIGGNH
                     KSNMELVAQGTANIFSSLFGGIPATGAIARTATNVKNGGRTPVAGIIHAITLLLIMLF
                     VGKWAALIPMATLAGILVVVAYNMSEWESFLSVFKGPRSDVAVLLTTFFLTVIIDLTV
                     AIEIGMILAAFLFMRKMIKFSDVSILTKDIDDSGPVTDKEAIGNFSIPKDAEVFEITG
                     PLFFGAAHKFKDAMKFIEEPPKILIIRMRRVPIIDATGIRTLEEVYKEAKHRGTKLIL
                     SEVHSEQVIGELKDSRLLFAIGKANVTDTFDEALQRCRNILSEA"
     gene            complement(10770..11042)
                     /locus_tag="PLO70_06205"
     CDS             complement(10770..11042)
                     /locus_tag="PLO70_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74087.1"
                     /translation="MSAEKYRQKLITEILIASTEEEVKKFCDTAMKNAAQNKGDGPAI
                     AWFINKLEEDLEGFNPRTKNVQQWSNIQTARSYIGIIKQRLSTLLH"
     gene            complement(11079..11381)
                     /locus_tag="PLO70_06210"
     CDS             complement(11079..11381)
                     /locus_tag="PLO70_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74088.1"
                     /translation="MVMTIDVNAETTELSKVLKLAEGYLSCERIIIQNIIKQLDNDST
                     EESIISYLEKLSAFLGNKIKTQEPDEDCTNYRYALGFVDTLLKMPYWKSWMKTIDI"
     gene            complement(11393..11998)
                     /locus_tag="PLO70_06215"
     CDS             complement(11393..11998)
                     /locus_tag="PLO70_06215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ74089.1"
                     /translation="MPLTGVRIFAEKWFASDEQFNQLYPLSIQALARRHWTPLSVARK
                     AAGFLAAENNVRILDIGSGVGKFCLAAAYYKPNAFYYGIEQRKSLVSHAEKAREILHL
                     QNVSFVNGNFTQLDFRKYDHFYFYNAFYENLAGTDKIDDSIDYSGELFNYYNRYLFKQ
                     LEKKPAGTRLATFHSMEEEIPQGYHVVGSEMDNLLKFWIKI"
     gene            complement(12026..12427)
                     /locus_tag="PLO70_06220"
     CDS             complement(12026..12427)
                     /locus_tag="PLO70_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74090.1"
                     /translation="MERSTNKDLYYTRDHEWIDFRGTVAYIGICRFKLLGFREVQQLV
                     FNEPSGFKKRGEVIATIRYNDYQIEAHMPVDGKVVQLNEVLTSGDQNMLLHYPENNGW
                     IAMIIPAQPYERKDLLLPQQYQMNGKSKYAK"
     gene            12529..13236
                     /locus_tag="PLO70_06225"
     CDS             12529..13236
                     /locus_tag="PLO70_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ74091.1"
                     /translation="MSIKGLFPIDKWDFKSESVLTDLPLPDYELLTTNKTEQVYKKGE
                     IIFREGAYPSGIFYITEGKVKKYKVDKDAKEHIIYVANIGELLGYHAILSEDRYPDSA
                     AALEESKIAFIPREDFLTTLHQSEILSRRLLKTLSHEFAVLANSLTMFAQKSVRERLA
                     LQLIVLREKYKVNFQPGMPVEINMSRDDLASLVGTVRENVVRVLSEFKETGIIATKGR
                     KIIVHDVNKLIKIADYK"
     gene            13300..14223
                     /locus_tag="PLO70_06230"
     CDS             13300..14223
                     /locus_tag="PLO70_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ74092.1"
                     /translation="MMKITLSFTLLLLTVTTFSQLPSVSSGTLKRHESFYSHYITARH
                     IDVWLPEGYSVTRKYSVLYMHDGQMLFDSATTWNKQTWDADDVITKLLQENKINDIIV
                     VGIWNGGTTRHTDYFPQKPFESLILEQKEKIFTAARANGNSVFNEQKINSDNYLKFLV
                     KELKPFIDKNYSTYKDRSHTFIAGSSMGGLISMYAICEYPKVFGGAACMSTHWPGIFS
                     MEGNPIPDAFINYLKTNLPDPKKHKIYFDYGTATLDALYPPLQQKADEVMKEKGFTGK
                     NWITKEFPGEDHSEKAWHKRLHIPLTFLLGK"
     gene            14262..14711
                     /locus_tag="PLO70_06235"
     CDS             14262..14711
                     /locus_tag="PLO70_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HQZ74093.1"
                     /translation="MKNIFYLLGILFFLYSCGTNKTEEPEIPVQEVYMWQAGMNDSTG
                     LLEMTKVMSSDIDSVSVPSVLSYFNTTNNNIKLDLVKISGDTIYLQITDAAYLTQQMG
                     SAGPTTYIAGLVYNLTEIPGISYVNLDFEEGDHAQPGTYNRENFKDN"
     gene            complement(14763..15737)
                     /locus_tag="PLO70_06240"
     CDS             complement(14763..15737)
                     /locus_tag="PLO70_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQZ74094.1"
                     /translation="MKKLIAFAAICISIIAAAYFSSCNNQDAGTEAKNRKEDSLRKVV
                     ERGDYLANHVAACIHCHSKRDFSKYSGPVIPGTEGGGGDVFDNTILDAIPGTLYGKNI
                     TPDNETGIGSWTDDEILRAITHGIRKNGDTLFPIMPYVNFNKMAKDDLLSIVAYLRTL
                     KPINNKVKDRQLMIPISMAYPAPALQNAVDNNVRPPESDMVKYGEYLSTVADCATCHT
                     PFVKGQLDFARAYAGGNTFNLGECRVNSANITSDSATGLGTWTEERFMNKFTVYRDEK
                     AYNFNPGKQNTVMPLSDYAGMTDYDLKAIYAYMRTVKAVSNQVEKFPK"
     gene            complement(15816..16922)
                     /gene="nagA"
                     /locus_tag="PLO70_06245"
     CDS             complement(15816..16922)
                     /gene="nagA"
                     /locus_tag="PLO70_06245"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQZ74095.1"
                     /translation="MPERKIYSANRIFTGESWLENHAIITEDGQIKKLIPLTSLPSSQ
                     HTRHFENCIIAPAFIDLQIYGAFGKLLAVYPETDALVKLKDYCEKGGTAYCMPTVATN
                     TYEVFYKCIDAIRAYWKRGGEGIMGLHIEGPWINPVKKGAHIESLIHSPTLQQAKELI
                     EYGKGVIKMITLAPEVCTREVIDLILSHHIIISAGHSNATYNEARTGFANGITAVTHL
                     YNAMSPLQHRAPGLAGAAMDDSSVMASIIPDGYHVDFAAIRIAKKVMRDRLFVITDAV
                     TATTEGYYKHVAAGDKYESAGILSGSALKMNEAVKNLVNHCGIETGEALRMCSFYPAG
                     VIGAAGKLGKLLPGYTAAIVVLDNNLEVVSIFRQ"
     gene            complement(16969..17658)
                     /locus_tag="PLO70_06250"
     CDS             complement(16969..17658)
                     /locus_tag="PLO70_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74096.1"
                     /translation="MSQTKILYVEDELFLGKIVRESLESRGFEVIMEGDGAKVIDLFK
                     KSKPDICVLDIMLPNKDGFTIADEIRELDEEVPIIFLTAKTQTEDVVKGFSLGGNDYV
                     RKPFSMEELIVRIQNLLRTKTEAPKKISGGTASFGKYTFQLNRQVLSNGKEERKLSFR
                     ESELLKLLYENRDKIIDRKDILNLLWGNDSFFNSRNLDVYITKIRSYLKDDPSLEIIT
                     IKGIGYRFVAG"
     gene            complement(17655..19139)
                     /locus_tag="PLO70_06255"
     CDS             complement(17655..19139)
                     /locus_tag="PLO70_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQZ74097.1"
                     /translation="MKRRKLIPDPVLMVISILGIAAFLFYWLKQSYEREEKSLAIKTE
                     MLFRETVMKLQVSKLKLDGVMIDSTGKGGLKIFIGEDDKRKVRFRPKEEIVSTINVIR
                     DKLTDSLKKDTAKGKKGMVISMQTSSTFETEKDSLKFNMDLPGPPPGPGNHIFNFLYG
                     VDSLQDPLRKSEIDSAYKLALQKQKLDIPFTIIENDGKEESDGPAFSSITIGLTHPVT
                     YTLELGNTFPYLIKRITLPILFSVLLLGITVLAFVLLYKNLLKQRKLGELKNEFISNI
                     THELKTPIATVGVAIEALKNFNAIDNPQRTREYLDISQNELQRLGLLVDKVLKLSMFE
                     KQEIELKTETLNLRGVVTEVVESLKLQLEKYHATVSVTHEGELNIQGDRLHLLSVVFN
                     LLDNALKYSKEDPVIQIDLKGDEDNVLITVKDHGIGIAPEYKDKIFDKFFRVPHGDTH
                     DAKGYGLGLSYVAQVIKKHKGTIAVQSEPGTGTTFTITLPKHTS"
     gene            19268..20077
                     /locus_tag="PLO70_06260"
     CDS             19268..20077
                     /locus_tag="PLO70_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HQZ74098.1"
                     /translation="MKRYLLTACSVCLAMIAIQAQQKEGKVIYERTVKLQFSFAGMPG
                     GMEQQMPSSRTDKFELTFGNNQSLWKQAEQDNTDDEGPSFGGEGMQVRMVVAGSNDVL
                     YSNFDNGRRVEKRELFDKTFIVDDTISKLKWKMTGETKSILNHNCMKAVAVRTSKRMM
                     MNMDNGVMERKEVDDTANIVAWFATDIPVSAGPSEFQGQLPGLILELDVRDGDQVYKA
                     TIISEKADLAVIKEPTGKKKYTQAEFKKERDKMMEEMNRNNQGGQRIIRMN"
     gene            20225..21016
                     /locus_tag="PLO70_06265"
     CDS             20225..21016
                     /locus_tag="PLO70_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQZ74099.1"
                     /translation="MIQQDFAYRVAELVKKDDAVIGLAAGGSWITNEIDEYSDLDLIL
                     ITKIKIAGDKEKMMDYARHFGDLISGFTGEHVGEPRVLICLYDKPLLHVDIKFLTLPE
                     FYDRVENPVVLFERNNQLSDIINSTNAVWPQPDFQWIEDRFWTWVHYACLKIGRGELM
                     EAFDFLSFLRMTVLSPLLQVKNNKQARGLRRVETALSLSDLENLKITIAQYTRPSVIK
                     ALDNTISIYKSLRRKIYPDTVCLQAEAEKKATAYFKTIKSKNLPG"
     gene            complement(21035..23005)
                     /locus_tag="PLO70_06270"
     CDS             complement(21035..23005)
                     /locus_tag="PLO70_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQZ74100.1"
                     /translation="MSSQHKVSVAGLLIALGIIYGDIGTSPLYVLNAITNGKTITREL
                     VTGALSLVIWTLTLQTTVKYVILTLNADNKGEGGIFSLFALVRRRKKWLVVPAMVGGA
                     ALLADGMITPPISVTSAIEGLKQVPFLSTISQTTIIYIVIGILTLLFFIQQFGTAFIG
                     KFFGPVMAVWFLMLAVLGFIHLSDDLSILNSFNPIYGIRLLIEYPQGFYILGAVFLCT
                     TGAEALYSDLGHCGKWNIRNSWIFVKTCLIINYLGQGGWLLSHFEGKTITADMINAGF
                     NPFYGVMPGWFLYFGIAIATAAAIIASQALISGSFTLVSEAMRLNLWPKLKINYPTET
                     RGQLYIPSVNLLLFLGCTGIVLYFQKATNMEAAYGLAITLCMISTSILFSNFLVLKRT
                     KSVWIYLYLVVFLLIETSFLLANIQKFPHGGYVALIVGGLLFLVMYIWFKARRIKNRY
                     VEFVRLEHYLSKIQELSNDRSVAKYATHLVYLTSADNPNEIEHKIIYSILNKKPKRAD
                     IYWFVHVDTVDNPYNCEYKVEHIIPNEIIRIDFKLGFRIQPRINLMFRKAVEDLVKNK
                     EVNVISRYESLASSNTVGDFQFVVMEKYISQDGELPFFEKILMRFHFWLKDISLSEEK
                     GFGLDLANVTVEKFPLVVGEMPNLKLKRIYNE"
     gene            23185..24294
                     /locus_tag="PLO70_06275"
     CDS             23185..24294
                     /locus_tag="PLO70_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ74101.1"
                     /translation="MSAQFFISIALVILAAAGCFLVSAYVDYKVVAFILLLTVSLVAI
                     VFDIVPVLVAATLSALIWNFFFINPRFTFHIGSAEDGLLFLMYFVIALINAVLTNRLR
                     RIEKQVREKEEKEKTVKLYNTLLNSLSHELKTPIAAIVGATDNLLAVSSRLSEENKKE
                     LVEGISVATLRLNQQVENLLSMSRLESGFIQPKKDWTDMNELVYDVVHRLEQQLKDHK
                     LNISIEDNLPFYKLDYGLIEQVLYNLLYNAAIYTPEFSDILVQVKNNTDKTYTLDTGD
                     KAPSSVKNETNSLVIIVEDRGPGFPPEEISKVFDKFYRLSNARTGGTGLGLSIVKGFV
                     EAHNGTVILENVPHGARFTIDIPAETSYINNLKNE"
     gene            24287..24973
                     /locus_tag="PLO70_06280"
     CDS             24287..24973
                     /locus_tag="PLO70_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ74102.1"
                     /translation="MNNAAILIIDDEVQIRRLLEITLESNSFNVESAETAKDGLRIAA
                     NHPPDLVILDLGLPDEDGHSVLKKLREWYTKPVIILSVQSSEENIVKALDNGANDYLV
                     KPFRTGELLARIRSALRGMVSEENDPVVDAGDLQIDLAARTVKKNNETIKLTATEYKL
                     LSLLAQHEGKVLTHQYLLREVWGPGYISQSQYLRVFIAQLRKKIETDANRPEHIITES
                     GVGYRFIAKE"
     gene            24998..26959
                     /locus_tag="PLO70_06285"
     CDS             24998..26959
                     /locus_tag="PLO70_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQZ74103.1"
                     /translation="MKSNSIHSNRITIASLLVALGIIYGDIGTSPLYVLKAIVGEKQI
                     DEVLVLGGVSCVFWTLMLQTTVKYIWLTLKADNDGEGGIFSLYALVRRYGKKLVIPAI
                     LGATTLLADGIITPPISVASAVEGLEAVIPNLPTVPIVIIILSLLFFFQRFGTQKVGR
                     IFGPAMVIWFTMLFVLGFSQILHYPGILKSLNPYYAYELLARYPGGFWLLGAVFLCTT
                     GAEALYSDLGHCGRKNIRLTWAFVKICLLVNYLGQAAWIMHQGQPLLEGRNPFFEIMP
                     HWFLIAGIIIATAATIIASQALISGSYTLISEAMNLNFWPRVRVRQPSNLKGQIYIPS
                     VNAILWIGCVMMILYFRSSTHMEAAYGFSITITMMMTTILLSFYLIYKLKWNKWLVAG
                     ILILFATVETSFFIANVAKIKERWMFLFFELFIFMVMYTWFYARKINNKFTRFVDLGK
                     YAPTIKELSEDDNVPKFSTHLIYLTKANNRHQVEEKIINSIFAKKPKRADVYWFVHIN
                     RTEHPYTLSYDVSELLDDKVIKVTINAGFRIQPKTELYFKKIVQDLVIKKELNLHIRP
                     DGSTKYNAQPDFKFIVIEKFLSVENEFTVKDGLLLNSYFFLKELGQSDEKAFGLDKSD
                     VVIEDIPLVYQPSQHIELTRVNNYTNFAK"
     gene            26956..28035
                     /locus_tag="PLO70_06290"
     CDS             26956..28035
                     /locus_tag="PLO70_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQZ74104.1"
                     /translation="MKRVLLLVYLHIVTIAVTGQDSTNKNDLSFSGFAEVYYLYDFSK
                     PADGNRPAFNYSHNRHNEFNLNLGFAKASFATERVKANLAVAAGTYINANYAAEPGVL
                     KNIFEANAGFKLSKKKNLWLDAGILPSHIGFESAVSKDCWTLTRSILAENSPYFEAGA
                     KLTYTTDDGKWILSGMALNGWQRITRLSGNSLMSWGTQIQFKYSEKVLLNYSTFIGTD
                     KPDSARLSRFFHNIYGIFTITNKIGLILGFDIGTEEKSPGANSSNTWYAPVGILKYTI
                     NNKWAIAGRIEYYNDKNGVIIPAGNANGFQTTGYSLNIDYAPVKNVVMRLEGRTLNSM
                     DDIFSNGSAFTKNNTLVSSSIAINF"
     gene            complement(28055..>28571)
                     /locus_tag="PLO70_06295"
     CDS             complement(28055..>28571)
                     /locus_tag="PLO70_06295"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ74105.1"
                     /translation="GDMAVNTIKTLTLGPDLRLDINPTPKLNIGLGAGISYNNTKYSL
                     QPGFSNNYLSQEYTTSLDWEMPKGFFFATDFNYTINSQRAAGFNIKVPLWNASISKQM
                     LKFNRGELKFSARDLLNRNVGISRNTNNNYIEDSRVLTLRQFFLLSFTYSLSKTGLNN
                     TGGGGMRVIAR"
BASE COUNT         8243 a         6125 c         5453 g         8450 t
ORIGIN      
        1 gcataaggcc gcagcgctac tgctttctga taatatgatt gtgattgcgg ataatcctta
       61 ttcaggtaat ataaccagcc aatccgcagg ttcagttcat aacttttttc atcatacaat
      121 ttggccaggg tggcaatggc acctgtaaaa ttcctgacac tttcctgcgt gtaactttct
      181 ttaaaagcag cctgcagctt tgcttcctct tgtgcggtta caaaaagaga aagcagcatt
      241 gtactaattc caataattga tttttttaaa attcccatga taagcctcct gtaattgaat
      301 gttgattaaa atttatagta gtcccaaata attgtcgttg ctcatacgta taaccaagct
      361 gaaaaacggt gttcccgggc aaaaggaaaa taccggtaac accggctttc attttattgg
      421 gatcaatcgc attgtacaca taaagtgcat cattctcggc aaggttatga aaatcaccca
      481 gggtgatatt cccttccagc catatattct tcagcacttt cacgccaagc acctgtttaa
      541 aattaaaagc tgtcccttcc tgctgccgta ccatggcggt agaaaaactg tagagatcaa
      601 gattgccttt cggatacagg cctacctgca ggttatattg ctgcacagaa gtgtcagtca
      661 gtttaccaag gatagcatca gcctgcaggt cgaaataatt tccattgtat ttaaaaccaa
      721 caagcaacaa atgattgttg tatttgaaat tattgaacgg cgtatggaga tagtgatagg
      781 ctgcttttaa ctgccagtga cggtcaagat tgagcatgag ccggttgtaa tattctacct
      841 gcctgatgtt aatattgtta ttatgcgtaa ctgcggtcag taacggttca ttgatctttt
      901 gctggtaagt ggctacagac tgctgcatat gaaaaccatg actaaagcga tttcccaggc
      961 caacccttgc ataaaaagga ttctttcttg ttgttacaga ggtttgttta taactcaact
     1021 ccaatgctgc actggtgaga gctgccggct tactcttttt ttcctctatt gccccctttg
     1081 ataaatactt tacctctgcc atggcaatct ccggctggtt aagatttatc ctgctccagt
     1141 atatataata atgagctgtg ttgttatact ggtcctcttt cagcaccact tcatagtgcc
     1201 tgatagcttc gctgtaattg gtaagcataa atgctgcata accaagacgc aatctcagga
     1261 gcagaaaatc ctggttattg acaatagctg tttttccata attcaataaa gctttccagt
     1321 ctgctttttc atataaagca aatgactgac tgtcgacagt ggcaaaggaa aaatcctgtt
     1381 gtgcctttgt atccaaaaca gccaggaaga aagcaagggc ggttgtgatg atcagtttcc
     1441 gcatatagct tcaatttgtt tgttacttaa ttccacacga aatgcctgta gctttccgtt
     1501 ggttattact acccgggcag tcattttttg ttgcaggtta ttacccagtt gctggtcggc
     1561 ccggctttcc agcacaatgg ttccgcctcc catcatatta tcatcctgtt ttacatggct
     1621 ggtatatttc atcttcataa ataaataaaa tggagccagc aggtcttgta tccatttcaa
     1681 aggatgcctg ccgaatatat tttgcggata cgtatcagtg attgtcactt ttttatcagt
     1741 actcagcact actttgtaag cagctttata aaaatgatac agcaaggagt ggtgattgcc
     1801 aaaataattg gtgaaataga acatcgtacc gttattgata aaataggcat aggcatgctg
     1861 ctgtctgcaa tagaaatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     1981 tacgatatct ccttttttta tataagcgcc tttggccact ttaaaagaac ccttctttaa
     2041 atgtgatagc ttagaataaa gcccttctgc atgtttaatg acaatagtat tgccccagtt
     2101 ttgcagtaca ttatttccac caacaggatt atcatctacg tagtcgatta tttcttccac
     2161 tattccgtcg gccggactta ataccggttt attataacaa tagaattgct ccggcaaact
     2221 gcctgaaccc tggtaagttt ttttttcttt atcggttatc acaaagtcta gtgctttgcc
     2281 ccaatcgcct ttatgtgtga tgctgccatc ataaccctgg ctcactgtcc attcacccat
     2341 aaaaggaagg gagagatgat agtaataaaa actattccac actctttctt tattattggt
     2401 ataccggtac aggttttctt ccgggctgta gtattgaatg ggtgtcagta ccattttacc
     2461 gcctgtcctt cttaattgca ggacatatag aaaaagaata acggtgatac aaaaaggcat
     2521 ggagaaaaca ggtaatcccc aggtgtaggt gatcttccaa agggctataa caagtatata
     2581 agaaataggc accgtgatca gtgaccagaa aaaagaacgg acggagggaa tgatataaaa
     2641 cccgccgagt gccactgata ccagcatgaa gttggtaccg aggttataat agttaacact
     2701 gccggcataa ccatccatca actggataaa tacaatagca atagcatagc cggcgatcat
     2761 cagcgttaac gcaatacggg aaaaaaagag cagcgctata gtaagcacta tacctgctgc
     2821 aatgttactt tgaaataaaa tggcactcaa tgaccgtaag taacccgtta ccacatcggg
     2881 aagcgacagg ttttcaaacc attgaaccaa cctgatcaga tccgtacccc cggtggcata
     2941 catttcattc aaccagtaaa tatttcgctg cgtaagacca attacggcaa attccctgga
     3001 tgaaagaata acaacccaca gtgtaaaaat aaatgccagt gataaagccg gaagattgct
     3061 tttcccgagc ttagcaataa acgctgcaga taataaaact gaaagcactg ctgctaatgc
     3121 cagcaatatc cagaaccctg cactaaactc ataaaaagtg cccatgccca gtcccaccag
     3181 cacagcacta taggtataaa gaccactctt cacctgctca cggctaaagc caataaaata
     3241 agccaccagc acggcaacaa gagaagcaat aaaacctgcc aggcctgtat aaggattaaa
     3301 aaaagttatc agtaacacta ctgatgcaaa atattttttg ttactgaaga acaacatgga
     3361 atagctgttc agaactgtag tagtgatata gctgagaaat aatatgaacg ttgttctttt
     3421 cacttcaaca ggcgtaaagc cggtttatca tatattaatt gtttgcaggt gttccggaac
     3481 tttttccggt tgctccatat attgcaatgt ttctgcctcc cgtattacat gtgttttgcc
     3541 ttgctcatca atcagcacta cgtttggccg catctgtata aattgcatcc actgcgtcat
     3601 attatacgct cccactttgt gtacaaccac cttatcgcca cgattcagca gtggcaggtt
     3661 gatactttgc cgtaccacat caatattcat acataatggt ccatacaatg ccatgtcttc
     3721 agtatgctgt gtaaaatcct gtgccggaga tattttatgg tcataccaga aagaagtgaa
     3781 taacaggtta acccctgcat caagaatggt ggcacgacgg ccatcgctta atcgtttatt
     3841 ggctaatacg gttgacagca ggtagcctgc atcatctatt agcacacgac cgctttctaa
     3901 tactaacaac ggcaattctt ctgtgctaaa tccgagtccg agtaaattcg ttgtgatagc
     3961 ttctgcaaat tcgtccaccg atgggacact gtccgttcct ggtagaaaag aaccccgcaa
     4021 cgtgttagag gaaggaaaac cgccccccag gtctatatac tggataggcg tatttaattg
     4081 tgttttacat ttccatgcca ggtcgcccaa tttttttaca gcggtcgcat aggcattggt
     4141 agtgagcata aatgttccga tatgacaatg cagaccggtc aattccattt tttcggactc
     4201 cacaattttt gtaagtgcat tcaaagcctg gccattttca taattgaacc caaagcgatc
     4261 ccacatggga tacactcctg tatccatatt tacccggatt gccactcttg gctttttttt
     4321 caacgtatca cttagttcaa tcagctggaa caattcatca aagtgatcga tatggataag
     4381 agagccgttg gtaacagcca tctccagttc ttcatttgtt ttctcgggac cgttgaaaat
     4441 aattttatta cccggcactc cattacccaa tgccttacgg tattcaaaac cacttactac
     4501 ttcggcccag ctgccttcct ggtgaaatat cctgcagacg gaattcaggt aattggtttt
     4561 ataactccag gcaaactgta ctttgggata acgggttgta aaagcacgga ccgcatgctg
     4621 atagttgtta cggatcgttc tttcgctcag gacaaataag ggagaaccat actgtgccat
     4681 catcgtatgt acagatacac catctatttg ttttaccggt tcatattctg atcttgttcc
     4741 gaatttgttc atcaggcctg tattcatttt ttgaatagaa ggactttcat aaactaactt
     4801 tttcatactt cttcttttta ttacaattcg cccattccgc ttacctggtg aaactctttg
     4861 atgtcagtaa tatgatccca ggaataacgg ataaatattt tccccacttc atacgccgta
     4921 taaaacggtt gttcttcccc caatgccatt ttagccaatg ctgccggttg attttgccct
     4981 gctgcggctg tcaggtaaat ccatgccggg aagcggggat ttatttccat gatgtataat
     5041 tcatttttct ttgtccgcat gatctccagc tcacatccgc ctttccattg tgtggctttt
     5101 acaaaatcac tggctatttt tatcagtgta tcatcttcca gcgtaatgcc tgcccatgct
     5161 tttcctttat cggtgatata caattttcgc ataggaataa tgctgacagc atttcctttt
     5221 ccatcaccca atactgcaat atttatttcg gtaccattaa tatattcctg aacgatgacc
     5281 ggaaatcccc atttggcagt aagtttataa aatgcctttt gagcctgttc cagtgtatac
     5341 gccaccacgg catcataaaa ttttcctttt accaccaacg gaaaacccaa tgcctcttca
     5401 caggcattta gttcagcggg actgtttatt attttgtctt ttggaacaag caggttatgc
     5461 ttttcaccaa acttgtataa attaatctta tcacgggcat caaactgctc ctgcgaaggc
     5521 aggaatgtag ctatccccat attttttaat gcagggccca gcctgatgaa attatgcagt
     5581 tctgcatcga agttgggaat gataagttgt aactgttcct tttcatgaat gtattgcaat
     5641 ctcctgagta aggcttctga tcctgctgcc gggtaaggaa tttgatacgt cttatccact
     5701 acatcgtgca tgtagatacc cggttctaat gtttcataag acaatccgat aatcctgaca
     5761 ggtctatgga gaccttctct taatccccgg atcacagcaa taccagggcc ggggctatcg
     5821 atagcattta atcctgttac agcaattgtt aacaggttgt cgggcttttc cattcagtcg
     5881 tttttaatat taatctccaa gaagattggc ctctttcagc tgattcataa aatcatccca
     5941 atcctgttca agacgaaaag gatcaacttc atacttctct agtatttgtt ctttaaccga
     6001 agagctggct gtgccctgtt tcaggagttg tactatttca gcagcaacag gatttgcgga
     6061 gtatgaatct cctgttgagg gattgaagat gaacccggat tcactggtcg caatattttt
     6121 tttgagatac ataactgaca aattaatatt cataagacac cggaaataat gcactgttta
     6181 aagtgatcag cagtattcag atcaaaatga tcaatatcat atagcaggct gatccattac
     6241 catgacctta actttaccca gatataatca tccatcaata cttcattttt aaaaactgcc
     6301 ttacggcgaa ctccctcgag ataatacccg tttttttcga gtacccgcat agaagcctta
     6361 ttgttttcaa aaacttcagc ataaagccgg accaggtcaa aataataaaa tgcatattct
     6421 gtcatttggc ctacagccac tgtagcaact ccctgtcccc agtatttttc accgatccaa
     6481 taacctatct cggctgactt actatagaca tccccattca gtattaatcc aattcccccc
     6541 accatctgtc catccacatc tatagccata ttaatagccg gcactgcatc cactactttt
     6601 tccagccagg cctttccatg ctcttctgta taaggatgag gaaaataatt acgaaggttg
     6661 ttccacacat ttttattatt tgcatgaaaa accaggttat ttaaatcttc ctgtttccag
     6721 tttcttattg tgattgtcat accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnncaaaca ttattcttat ttgtatcaac ataggcggga ctgtttttat ttgttccggg
     6901 agtaccgtta ccgggttttg tcgtttgagc agataacaga ccgctcatag caatcataat
     6961 aactgctgtg aaaatcatct ttttcatttt tcgatattta attgttatta atcgttttat
     7021 tcttttccca tcctcttcca cgatgctggt ttggagctgt agttattcct tcatttataa
     7081 agagtggttg aaaaattttt tctaattcag gccgctgctc cggagaacag atctttttgg
     7141 tatttaaata gaaactgtac gtttcatgct tcagctgacc atgcaattcc ccgatttgcg
     7201 ctgacatact gttcaaggcc agtgtgtcgg gctgattttt ttgcatttcc atgaacatgg
     7261 cattttttaa agaatcaatt tcaaaagtca gactcatggc tacaggccgg aacgcctggt
     7321 ttatttcacg gaaagcactc atttgtgatt gatcaaaacc aagtgtttgc cggagaaacc
     7381 ttccattgat catatttccc ccgtaagaac cagcaataac gacatcatca ctttttttgc
     7441 tttccctgta attatgataa actatcgttc cgattgtaac tgcattggtt accaacagca
     7501 gcacaaccgc ccatgtcaat atctgtgtct tacgcatcat acctttcggt tattcattta
     7561 ccatttgttc ataaaaccca aaatgctcca tattgttatc gttcatcagt acaacaccag
     7621 gcctttcatt acccgattga taaagactgc caatagtaat gcctgtaaat actgcaacca
     7681 ctaaccccgc cccaatcatt actgttttcc acacaggtga aattcttttt tcattctgta
     7741 ttccattttt agcaatagca gccattacac gggttgacag aaaaggattt gattccatat
     7801 tccgctcttc gccgatgaag ttttcaagta actgctgtat atctttctcc tgtttcattc
     7861 ctgttgattt ttatatttag acaattagtg taattaaaat cctatggcct gtccagctct
     7921 ttttccagtt tattcctgag attggttttg gcccgtataa ccagttgttc cactgctcct
     7981 tcactggtat gcataatttc agccacctgc ttttgcggga gctcttcata tttggtcaat
     8041 acaaaagcca gttgttgctt ttccggtagt gcatatatag cctgccggat gattgctgaa
     8101 tcagattttt catccatcag tgtttccgcc tgcttgtctt ttgcggctac ctggagcaat
     8161 tgagaaattc cggtccagaa cctgttacgt ttttttttct ttaaaaaatt aaggcttgta
     8221 ttaatagtga tcctataaag ccaggtggaa aaggctgact taccattata agcagaaatt
     8281 gactgataga cttttacaaa tacatcctgg gtaatttctt ccgcatcttc tttactgtga
     8341 agcaaaccca tagccacacg aaatacattc ggctgatatt tttttatcaa ctgttcaaaa
     8401 tcacggtggt taccgttgag aatactctct ataagttcct cggtctgcat ctctcatacc
     8461 ggttttttaa aagcccgtcc tgatgctatt gttcagcccg tcaataagat attatactca
     8521 ttcgtctttc ctgatcaaaa gctaatgagt aaatccggga tttcatcttc acattcgcta
     8581 cactttcatg taattacgta aaattatagg tattaatgtg taacctttgt caccttatcc
     8641 ggagttcggt aaagacctgc ccgcctgtat gtttttctgc accgtggtca annnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagtggtt tctgttatca acttcagtga
     8821 taaacctgta gctgtaaaat tgaattccag attccaggtt ggcacttata cagaatattt
     8881 caccggcata aagtatgagc tgaaaggaga tgatgaactg tctttacctg cctgaggtta
     8941 tttagtgctg gcaaaaaacc agtaaataat tatttcctga tcagtttgag gctgacataa
     9001 aaacaaattt gtcatattga gcaaagccga aatatgacaa tgagggggac tttaaagaga
     9061 acctcaaccc ttttataatc tatatctcat ttttgcattt tatgcttcag acaatatatt
     9121 tctgcaccgt tgcagggcct catcaaatgt atcagtaaca ttggcctttc caatggcaaa
     9181 cagcagcctt gagtctttta attctcctat tacctgttca ctatgaacct ctgacaggat
     9241 caattttgtt ccccggtgct tcgcttcttt atacacctct tccagtgttc gtattcctgt
     9301 tgcatctata atcggaaccc gtctcatccg tatgataagg atttttggag gctcctctat
     9361 aaacttcata gcatccttaa acttatgtgc cgcaccaaag aataaggggc ccgtaatttc
     9421 aaatacttca gcgtctttag gtatggagaa attgccaatc gcttctttat cggtaaccgg
     9481 gccactatca tcaatatcct ttgtcagtat actaacatca ctgaacttaa tcatcttacg
     9541 cataaacagg aaagccgcca ggatcattcc aatctcaata gcaactgtaa ggtcaattat
     9601 tactgttaag aaaaaagtgg ttaataatac agctacatca cttcttggcc ctttaaatac
     9661 agacaaaaaa ctttcccact cactcatgtt ataggcaacc accaccagga tgcctgccag
     9721 tgttgccatg ggaattaacg ctgcccattt accgacaaac agcattatta ataaaagggt
     9781 aatagcatgt attattccgg caactggtgt tctacccccg ttttttacat tggtagccgt
     9841 acgggcaata gcacccgttg ccggaatgcc gccaaacaac gatgaaaaga tattagcagt
     9901 gccctgcgcc accagttcca tattggattt atgatttccc ccgatcatgc catccgcaac
     9961 aacagctgat aacaatgatt cgatccctcc cagtaaagca atagtaaaag ccggttgaat
    10021 taattctttt atcgttgcaa aatctatatc aggaactaca ggtgttggaa gggaagaggg
    10081 tatgctgcca aacctgctgc caattgtttc taccggaagg ttaaaccatt gcatggcagc
    10141 tgtggtaacc aaaatagcaa ttaaagaacc cggtatttta tgtgtaatct ttggccagaa
    10201 taaaattatc agcacggtgg ctactgccag caaaaccgca tatacattca ctgtccggaa
    10261 gttggcaaca taacttatcc atttatccaa aaaatcggca ggcacaactc ccatctgtaa
    10321 tccaaaaaaa tctttcattt gggaagaaaa aatgatcagt gcaattccac ttgtaaaacc
    10381 gataatcaaa ggatgcggaa taaactttat tacagaaccg aggcgggcaa agcccatgat
    10441 gatcagcatg atcccggcca taaaagtggc aatgatcaat ccatttactc catgaacctg
    10501 cacaatgcca tatacaatta ctataaatgc gccggtagga ccaccaattt gcaccctgct
    10561 tccgcccatg gcagaaataa tgaacccggc aatgacggca gtgtataacc ctttttccgg
    10621 tgatacacct gaggcgatag caaaagcgat ggcaaggggt agtgccacaa tacctacaat
    10681 caatcccgcc attaaatccc tgctaaactg ctggcggcta taactcttta atgtgtcaac
    10741 tatttttggc ctgaacatac aatatcaaat taatgcagca aagtgcttag ccgctgcttt
    10801 attataccaa tataactcct ggctgtttga atattactcc actgttgtac attcttggtt
    10861 cgtggattaa atccctccag gtcttcctcc agtttattaa taaaccaggc aatagcagga
    10921 ccatcccctt tattttgtgc tgcatttttc attgctgtat cacaaaattt cttcacctcc
    10981 tcctctgtac tggcgattaa tatttcagta ataagctttt gacgatattt ctctgctgac
    11041 atggacatga ctgattattc cgggtttaat taaaaaaatt atatgtcaat agttttcatc
    11101 cagcttttcc aatacggcat tttcagcagc gtatcaacaa atccgagggc atacctgtaa
    11161 ttggtacagt cttcatcagg ctcctgtgtt tttattttat tccccaaaaa agcagaaagt
    11221 ttttccaggt aactaatgat gctttcttca gtactgtcat tatccagttg ctttataata
    11281 ttctgaatga ttattctttc gcaacttaaa tatccttccg ccagcttaag aaccttactc
    11341 aattctgttg tttcggcatt tacatcaatt gtcataacca tacctccctt ttttagatct
    11401 ttatccaaaa cttcagcagg ttgtccattt ctgaaccaac aacatggtac ccttgcggta
    11461 tctcctcttc catactatga aaagtagcca gcctggttcc ggccggtttt ttttcaagct
    11521 gtttaaacaa ataccggttg taataattaa ataattcacc ggaataatca atactatcat
    11581 ctatcttatc tgtgcctgct aaattttcat aaaaagcatt gtagaaataa aaatgatcgt
    11641 acttcctgaa atctaattgt gtgaaatttc cattcacaaa agaaacattt tgaagatgca
    11701 gtatttccct ggctttttct gcatggctga ccaaactttt ccgctgctca ataccataat
    11761 aaaatgcatt tggtttataa tatgcagccg ccaggcaaaa cttaccaacc ccgcttccaa
    11821 tatccagtat ccgtacgtta ttttctgctg ctaaaaaacc agccgccttc cttgctacac
    11881 tcagcggtgt ccaatgccgg cgtgcaaggg cctgaatgga taacggataa agctgattaa
    11941 actgctcatc cgaagcaaac catttttccg caaatattcg tacacctgtc aacggcatat
    12001 gtttctgctt tacttttcgt aagtattatt ttgcatattt actcttgcca ttcatttggt
    12061 attgttgcgg cagcagtaaa tcttttcttt catagggttg cgcaggtatg atcatcgcaa
    12121 tccatccatt gttttccggg taatgcagca acatattctg gtcgccggag gttaatactt
    12181 cattcagctg taccaccttg ccatccacag gcatatgtgc ttctatctgg taatcattat
    12241 accggatggt agcaattact tcaccccgct ttttaaatcc tgatggttcg ttaaaaacaa
    12301 gttgctgcac ttctctaaac cccagtaatt taaaccggca tatcccaata tatgcaaccg
    12361 ttccccggaa atcaatccat tcatgatccc tggtatagta caggtctttg tttgttgatc
    12421 tttccatatg agtgcaaagt tctgttaccg aaagcagctc cgcggtaaac cttatcactg
    12481 caaaaagtga gttttatcaa tttttaaacc gataaatttg tacacagtat gagcatcaag
    12541 ggcctgtttc caatagacaa atgggatttt aaaagcgaat ccgttttaac agaccttcct
    12601 ttacctgatt atgaattgct tactactaac aaaacagagc aggtctataa aaaaggagag
    12661 atcattttca gggaaggggc ttacccctca ggtatatttt atataacgga aggcaaagta
    12721 aaaaaataca aagtagataa agatgcaaaa gagcatatta tctatgtagc taatattggc
    12781 gaactgttag gctaccacgc tattctttcc gaagatcgct accctgactc tgctgctgca
    12841 ctggaagaaa gcaaaattgc atttatccca agggaagatt tcttaactac actgcatcaa
    12901 tctgaaatat taagcaggcg gttattaaaa acattaagtc atgaatttgc tgtattagca
    12961 aacagtttga ccatgtttgc ccaaaaatct gtccgggaga gattagcgct tcagttaatt
    13021 gtactgagag aaaaatataa agtcaatttc cagcccggga tgcccgttga aataaatatg
    13081 agcagggacg acctggccag tctggtgggc acagtaagag aaaatgtggt acgggtatta
    13141 tcagagttta aggagacggg cattatagca acaaaaggca ggaagatcat cgttcatgac
    13201 gtaaacaaac ttattaagat tgcagattac aagtaaagta gtaatgaata ttgcccgttt
    13261 acgtcaaaaa gtattcagtg aacctataat ttgtccttta tgatgaaaat aacgctgagc
    13321 tttaccctgc tccttcttac agttaccact ttctctcagc taccatccgt ttcatcgggt
    13381 acattaaaac ggcatgaaag tttttattcg cactatataa cagcccggca tattgatgtg
    13441 tggctccctg aaggatattc agttaccagg aaatacagtg tcctttacat gcatgatggt
    13501 cagatgttgt ttgactccgc taccacctgg aacaaacaga cctgggatgc agatgatgtg
    13561 ataacaaaac tgcttcagga aaataagatc aatgatataa ttgttgtggg tatctggaat
    13621 ggaggtacaa caagacacac tgactatttt ccgcaaaagc cttttgagtc attaatactg
    13681 gagcaaaaag aaaaaatctt tacagctgcc cgggcaaatg gtaattctgt ttttaacgaa
    13741 caaaaaatca attcagacaa ctacctgaaa ttcctggtaa aagaactaaa gccttttata
    13801 gataaaaact attccactta caaggatagg tctcatacgt ttattgccgg cagcagtatg
    13861 ggtggattga tttctatgta tgccatttgt gaatacccga aagtatttgg cggtgcagct
    13921 tgtatgtcta cacactggcc cggtatattt agtatggaag gaaatcccat tccagatgcg
    13981 tttataaatt acctgaaaac aaatctacct gatccgaaaa agcataaaat ctattttgat
    14041 tacggcactg ccacattgga tgccttgtat cctcctttac agcaaaaagc agatgaagtg
    14101 atgaaggaga agggctttac cggtaaaaac tggataacaa aagaatttcc gggcgaagac
    14161 catagcgaaa aagcatggca taagcggtta catattcctt taacattttt attgggtaaa
    14221 tgagctttta tgcgacttac ctttgcaaca aatcacccac catgaaaaat attttttatt
    14281 tattgggaat actcttcttc ctttattctt gcggcaccaa taaaacggaa gagccagaaa
    14341 taccggttca ggaagtgtat atgtggcagg ccgggatgaa tgattcaacc gggttgcttg
    14401 aaatgacaaa agtgatgagt tcagatatag attctgtttc tgttccttcg gtacttagtt
    14461 atttcaatac caccaataac aatattaaat tagatcttgt aaaaatatcc ggtgatacta
    14521 tttatttaca aatcaccgat gctgcttatc tcacacaaca aatgggttct gccggcccaa
    14581 ccacttatat agccggcctg gtgtataacc tgacagagat acccggtatc agctatgtga
    14641 accttgattt tgaagaaggt gatcatgctc aaccgggtac ttacaacagg gaaaacttta
    14701 aagacaatta aaaacccgcc ctgccaattg cgggacgggt tggttaatca gtgctcacct
    14761 ttttatttcg ggaatttttc tacctggtta ctgacagctt ttactgtacg catatatgca
    14821 tagattgcct tcaggtcata atcggtcatt cctgcatagt ccgacagtgg cattacagta
    14881 ttttgtttac cgggattaaa attatacgcc ttttcatccc gatacacagt aaacttattc
    14941 ataaaccttt cttctgtcca ggtgcccagc ccggtagcag aatcagaagt tatatttgct
    15001 gagttcactc tgcattcacc caggttaaat gtattgcccc cggcataagc acgggcaaaa
    15061 tcaagttgcc cttttacaaa aggtgtatga caggtagcgc agtcagctac cgtactaagg
    15121 tattctccgt acttcaccat atcactttca ggtggacgaa cattattatc aacagcgttt
    15181 tgtaaagccg gggcgggata agccatactg attggtatca tcagctgcct gtctttaact
    15241 ttattattta ttggcttcag cgttctgagg taagcaacta tagataataa atcatctttt
    15301 gccatcttat taaaattaac atacggcatt atcgggaaca gcgtatctcc atttttcctg
    15361 atgccgtggg tgatggccct taatatttcg tcatctgtcc aggaaccaat tccggtttca
    15421 ttatcagggg taatattttt accatataaa gtaccgggaa tggcatcaag aattgtatta
    15481 tcaaatacat caccaccgcc accttctgtt cccggtataa ccggtccgga atatttacta
    15541 aaatcccgtt tgctgtgaca gtgaatgcag gctgctacat gattggccag gtagtcgccc
    15601 cgttcaacta cttttctcag ggaatcttct tttctgtttt ttgcctctgt gccggcatct
    15661 tgattattac aggaagaaaa ataagcagca gcaataatac taatacaaat tgctgcaaaa
    15721 gcaatcagct ttttcataat cgttggttgt ttaggttgct aaagaggtta aactcttaca
    15781 aaagatataa aaaaaatcag atatgacacc tctatttatt gacggaaaat agaaacaact
    15841 tctaaattat tgtcaagcac aacgatagca gcggtatagc cgggtaaaag tttacccaat
    15901 tttccagctg caccaattac gccggcggga taaaagctgc acatacgcaa tgcctctcct
    15961 gtttctatac cacaatgatt gactaaattt ttaacggcct cattcatctt caatgcagaa
    16021 ccgctgagaa taccagctga ttcatattta tccccggctg ccacatgctt atagtatcct
    16081 tcggtggtag ctgttactgc atcagtgata acaaacagcc tgtctctcat tacttttttg
    16141 gcaatacgga ttgctgcaaa gtcaacatga taaccatccg gaatgatact ggccattacg
    16201 ctgctatcat ccatggcagc acccgctaac cccggtgccc ggtgttgcag cggactcatc
    16261 gcattataca aatgtgtaac agcagtaata ccattggcaa accctgtcct ggcttcatta
    16321 taagttgcat tactatgtcc tgctgaaata atgatatggt gagagagaat caaatcaatg
    16381 acctcccggg tacaaacctc aggggcaagc gttatcattt taataactcc tttcccatat
    16441 tctatcaatt ctttcgcctg ttgcagagtg ggtgaatgga tcagcgattc aatatgagcc
    16501 ccttttttaa cggggtttat ccagggccct tcaatatgca atcccattat cccttcacct
    16561 cccctcttcc aatacgctct aatcgcatca atacatttat aaaaaacttc gtaggtattg
    16621 gtagccacag tgggcatgca gtaagcagtt ccgccttttt cacaatagtc cttcagtttg
    16681 accagtgcgt ctgtttcagg ataaactgcc aataattttc cgaaagcacc ataaatctgc
    16741 agatcaataa aagccggagc gataatacag ttttcaaaat gccgggtatg ctgtgaagag
    16801 ggtaaagaag taagcggaat taattttttt atttgcccgt cttctgtaat gatagcatga
    16861 ttctccagcc agctttcccc ggtgaagatg cggttggcac tatatatttt tctttccggc
    16921 atatatgtac cggtaaaaat acaattgaaa tgaattggct atgaattttt atccggccac
    16981 aaagcgataa ccaatccctt tgattgtaat aatttctaat gagggatcat ctttcaggta
    17041 actgcggatc ttggtaatat acacatccag gttacggctg ttgaagaaag agtcattacc
    17101 ccagaggagg ttgagaatgt ctttacggtc aatgatctta tcccggtttt cgtataataa
    17161 tttcagcaac tcgctttcac ggaaagaaag ttttctttct tcctttccat tgctgagcac
    17221 ctgccggttc agttgaaaag tatatttacc aaaactggct gtcccgccac ttatcttctt
    17281 cggggcttct gttttagtac gcaggaggtt ctgaatacgg acaatcagct cttccatact
    17341 gaaaggcttg cggacataat cattgccccc caatgaaaaa cctttcacga catcttctgt
    17401 ctgtgttttc gccgttaaga atataattgg cacctcttca tccagctccc ggatttcatc
    17461 agcaatagta aagccatctt tattcggtag cataatatcc agcacacaga tatcaggttt
    17521 tgatttttta aaaagatcaa tcactttggc gccatctcct tccatgatga cttcaaatcc
    17581 acggctttca agactttccc tgactatctt tcccaggaag agttcatctt ccacatataa
    17641 gatttttgtc tggctcatga ggtatgtttg ggtaaggtga ttgtaaatgt tgttcctgta
    17701 ccgggttcac tttgcacggc aatggttcct ttatgttttt ttattacctg tgccacataa
    17761 cttaatccca gcccgtatcc tttcgcatca tgagtatccc catgcggcac cctgaaaaat
    17821 ttatcaaaga tcttatcctt gtactctggc gcaataccaa ttccgtggtc cttcactgtg
    17881 attagtacat tatcctcgtc accctttaag tcaatttgta taacagggtc ctctttgctg
    17941 tatttaagtg cattatccag caggttgaat accacactca ataaatgtaa ccggtctccc
    18001 tgtatgttca gctccccttc atgcgttacg gatacagtgg catgatactt ttccagttgc
    18061 agtttcaatg attccactac ttctgttacc acaccccgca ggttcagcgt ttctgtcttc
    18121 aattctattt cctgcttttc aaacatagaa agtttgagta ctttatccac cagcaggccg
    18181 agtcgctgca attcattttg tgaaatatcc aggtattccc gggtacgctg cggattgtca
    18241 atcgcattaa aatttttgag tgcttcgatc gccactccta cagtggcaat cggtgttttt
    18301 aactcatggg taatattgct gatgaattca ttttttaatt ctccgagctt tctttgtttc
    18361 agcagatttt tatacagcaa tacaaatgca aggaccgtta ttcccaataa cagaacggag
    18421 aacagaatag gtaatgtaat cctttttatc aggtagggaa acgtattgcc cagttcaagg
    18481 gtgtacgtaa ccggatgggt aaggccaatt gtaatggaac tgaacgctgg accatcagat
    18541 tcttccttcc catcattttc tataattgta aaaggtatat ccagtttctg cttttgcaat
    18601 gccagtttat aggcagaatc tatttctgat ttgcgcaagg gatcctgcag agaatctacc
    18661 ccatataaga aattgaaaat atgattgccc ggacccgggg gaggtcccgg cagatccata
    18721 ttgaatttca gtgaatcttt ttctgtttca aacgtggagg aagtctgcat cgagatgacc
    18781 atcccttttt tcccttttgc tgtatctttc tttaacgaat cggtcagttt atcccttatt
    18841 acattgatgg tagaaacgat ttcttctttg ggccggaagc ggacctttct tttatcatct
    18901 tcgccaataa agatctttag ccctcctttt cccgtactgt ctatcattac accatccagt
    18961 ttcagtttgg aaacctgcag cttcataaca gtttccctga acaacatttc agtttttatg
    19021 gccagtgact tttcttctcg ttcataactc tgcttcagcc agtaaaaaag gaaagcggca
    19081 atacccagaa tagatatcac catcaataca ggatctggta tgagtttacg ccgcttcatg
    19141 cagcaacaaa attagctatt cgtttccggc attatcaatg gctttaacct ttattaacct
    19201 taatgaaagg tgggttaact tattccggaa caggccttct atagctttgg gtcaaacaaa
    19261 taaaaccatg aaaagatatt tgctcactgc ttgtagtgta tgccttgcta tgattgcaat
    19321 acaagcgcaa caaaaagaag gtaaagtgat ctatgaacgt accgtaaaac tacaattcag
    19381 ttttgccggt atgccgggtg gcatggaaca gcaaatgccc agcagccgta ccgataaatt
    19441 tgaactcacc tttggtaaca accagtcttt atggaaacag gccgaacagg ataatactga
    19501 tgatgagggg cccagtttcg gcggggaagg aatgcaggta cgaatggtgg tagccggcag
    19561 taatgatgta ctctacagca attttgataa tggccgccgg gtggaaaaaa gagaactgtt
    19621 tgacaaaact tttattgtag acgatacgat cagcaaactg aaatggaaga tgaccggtga
    19681 aacaaaaagc atcctgaacc acaactgtat gaaagcagtg gcggtaagaa ccagcaagcg
    19741 aatgatgatg aatatggata acggggtgat ggaacgaaaa gaagtagatg acacggcgaa
    19801 cattgtggca tggtttgcca ccgatatccc cgtttctgcc ggaccttccg aattccaggg
    19861 gcaactgccc ggactcattc ttgaactgga tgtgagagat ggcgaccagg tatataaagc
    19921 cactattatt tcagagaaag cagatcttgc tgtgatcaaa gaacccaccg gcaagaaaaa
    19981 atatacccag gccgaattca aaaaagaaag agataagatg atggaagaaa tgaaccgcaa
    20041 taaccagggc ggccaacgga tcatccggat gaattaatca tcagcatatt tagccatgat
    20101 aaaacccttt gcgaatgcaa ggggttttta tttgtaaagc aatcaaaatt atctgtcctt
    20161 tactaactca tatgccgggt taacatcaac acttttttct atcttcagca acaataaatc
    20221 attgatgatc cagcaggact ttgcataccg tgtagccgaa ttggtaaaaa aagatgatgc
    20281 tgttattggg ttggcagcag gcggttcctg gatcactaat gaaatagatg aatattctga
    20341 cctggacctt atccttatca ccaaaataaa aattgcaggt gataaagaaa aaatgatgga
    20401 ttatgcccgg cactttggtg atcttatctc cggctttacc ggcgaacatg taggcgaacc
    20461 aagggtactg atctgtttat atgataagcc gctgctgcat gtagatatta aatttctcac
    20521 cctgccggag ttttatgacc gggtagaaaa tcctgttgtt ttgtttgaaa gaaataatca
    20581 actttctgat ataatcaact caaccaacgc tgtttggccg caacctgact ttcagtggat
    20641 cgaagaccgt ttctggacat gggtacatta tgcctgctta aaaatcggca gaggcgaact
    20701 gatggaagct tttgattttc tttccttcct ccggatgacg gtgctttccc ccttattgca
    20761 ggttaaaaat aataaacagg caagagggtt aagaagagtt gagacagcac ttagtttatc
    20821 ggacctggaa aatttaaaga tcactattgc acaatacacc aggccttctg taataaaagc
    20881 acttgacaat acgattagca tttataaatc gctccgcaga aaaatttatc cggataccgt
    20941 ttgccttcag gctgaagcag aaaaaaaagc tacagcgtat tttaaaacca tcaaatcaaa
    21001 aaatttaccg gggtgatcat tttttgaagg ataattattc gttataaata cgtttaagct
    21061 tcaggtttgg catctctcct acaaccagcg gaaatttttc tactgttaca ttcgccaggt
    21121 caagaccgaa tcctttttcc tcagacaggc tgatgtcctt taaccagaaa tgaaaccgca
    21181 tcaggatttt ttcaaaaaaa ggtagttcac catcctggct gatatatttt tccattacca
    21241 caaactggaa gtcgcctaca gtattactgc tggcgaggct ttcgtaccgg cttattacat
    21301 tcacttcttt gttcttcaca aggtcttcta cggctttccg aaacattaaa ttgatgcggg
    21361 gctgtatcct gaacccaagt ttgaaatcaa tccggatgat ctcattggga atgatatgct
    21421 ctactttata ctcacagtta tatggattgt caaccgtatc cacatgaaca aaccagtaga
    21481 tgtctgcccg cttgggcttt ttgttcaaaa tggaataaat gatcttgtgt tctatttcat
    21541 tggggttatc ggcacttgtc aggtagacaa gatgggtagc atacttggcc acagacctgt
    21601 cattgcttag ctcctgtatc ttagacaaat aatgttcgag ccgtacaaat tctacatacc
    21661 ggttcttgat ccttcttgct ttgaaccaga tatacatgac caggaataat aaaccaccta
    21721 ctatcagtgc tacataacct ccatgcggaa atttttgaat attggccagc aggaaacttg
    21781 tttcaatcag caggaaaaca accaggtata gatagatcca taccgacttt gttcttttca
    21841 ataccaggaa attggaaaag agtattgaag tggagatcat gcaaagagtg atagccagcc
    21901 catatgcagc ctccatgttg gttgctttct gaaagtataa aacaatcccg gtgcaaccca
    21961 gaaacaacaa caggttaaca gaaggtatat ataactgccc ccttgtctct gtaggataat
    22021 ttattttcag tttaggccac aggttcagtc gcatggcctc acttaccagg gtaaaggagc
    22081 cggagataag tgcctggctg gcgatgatcg ctgcagctgt tgcaatggca atgccaaagt
    22141 ataaaaacca ccccggcata acaccataaa acgggttgaa ccctgcattg atcatatccg
    22201 ctgttatggt ctttccttca aaatgactga gcaaccaccc gccttgcccc aggtagttga
    22261 tgattaaaca ggtctttaca aaaatccagg agttccggat attccatttt ccgcagtggc
    22321 ccaggtcaga ataaagcgcc tcagcaccag tagtacaaag aaacactgct cccagtatat
    22381 aaaaaccctg cgggtattcg atcagcagcc tgataccata aattggattg aatgaattaa
    22441 ggatgctcag gtcatcagac aggtggataa aaccaagaac agccagcatc agaaaccaaa
    22501 ccgccatcac cggtccaaaa aatttaccaa tgaatgccgt gccaaattgc tggatgaaaa
    22561 acaacagagt caggatacct atgacgatgt aaataatcgt tgtttgcgat atggtagaaa
    22621 gaaatggcac ctgcttcaat ccttctatag cggaggtaac agaaataggt ggtgtaatca
    22681 ttccgtcagc cagcaatgca gcaccgccaa ccatagcggg tactacaagc cattttttcc
    22741 gtctcctcac cagcgcaaaa agagaaaaga taccgccttc tcccttgtta tcagcattga
    22801 gggtaaggat aacgtattta acagtggttt gaagtgtaag cgtccagatt accagtgata
    22861 aagcgccggt aaccagttct cttgttattg ttttaccatt ggtgatggca tttaaaacat
    22921 acaatggaga agtaccaata tcaccataga taatgcccag tgcaatgagc agaccggcta
    22981 cggatacttt atgctgtgaa gacatcgggt gggtaaattg taattgtcaa aagtagaaca
    23041 gcaaaaaatc aaaaaaaggc attgacaaaa tctttataaa atctttacac ccctcattat
    23101 tcattttaca tcccacttat agcgccgggg ttacctttgt gttctattct ttattacatg
    23161 ttgcagcgtt taacaaaagt ccgcatgtca gcacagtttt ttatcagtat tgcgctggta
    23221 atactggcgg cagcaggttg cttcctggtt tcggcatatg tggattacaa agtagtagct
    23281 tttattctgc tgcttaccgt ttcactggtg gctattgtat ttgatattgt gccggtactg
    23341 gtggctgcta ccctcagcgc attgatctgg aatttctttt ttataaatcc caggtttact
    23401 tttcatatcg gctctgcaga agatggtttg ttgttcctta tgtattttgt tattgcactc
    23461 attaatgctg tactaaccaa ccgcctccgc cgtattgaaa aacaggttcg ggaaaaagaa
    23521 gaaaaagaaa aaacagtgaa gctgtacaac acattgctta attctttatc tcatgaattg
    23581 aaaacaccga tcgctgctat cgtaggtgcc acagacaacc tcctggccgt ttcatccaga
    23641 ttatcggaag aaaataaaaa ggagttggtc gagggtattt ctgtggccac tttgcggctg
    23701 aaccagcagg tagaaaacct gctcagcatg tcaagacttg agtccggctt tatacaacct
    23761 aagaaagact ggaccgacat gaacgagctg gtgtacgatg tcgttcaccg gctggaacaa
    23821 caattaaaag accacaaact caacatcagt attgaagaca acctgccatt ttacaagctg
    23881 gattatggat tgatagaaca ggttttgtat aacctcttat acaacgctgc tatttatacc
    23941 cctgaattct cggacatact ggtacaagta aaaaataata ccgacaagac ctataccctg
    24001 gatactggtg ataaggctcc gagcagcgta aaaaatgaaa ccaacagcct tgtgattatt
    24061 gtagaagacc gtggcccggg tttcccgccg gaagagatca gtaaagtgtt tgataaattc
    24121 tatcgcctga gcaatgcaag gacggggggc actggcctgg gcctttctat cgtaaaaggt
    24181 tttgtggagg cacacaatgg aacagttatt ctggagaatg tgccacacgg cgcccggttc
    24241 actattgata taccagccga aacttcttac atcaataatt taaaaaatga ataatgcagc
    24301 catactgata atagacgatg aagtgcaaat ccgcaggctg ctggagataa ctctcgaaag
    24361 caacagcttt aatgtggaat ctgccgaaac agccaaagac ggattacgta ttgctgccaa
    24421 tcatccgcct gacctggtta tccttgacct gggtctgccg gatgaggatg gtcacagcgt
    24481 attaaagaaa ttacgggaat ggtataccaa accggttatt atcctctccg tgcaaagcag
    24541 tgaagaaaat attgtaaaag cgttggacaa cggtgccaac gattacctgg taaaaccttt
    24601 tagaaccgga gagttactgg cccgtatccg ctccgccctt cgcggaatgg tgtcggaaga
    24661 aaatgatccg gtggtggatg ccggtgacct gcagatcgat ctggcagcaa gaacagtaaa
    24721 gaaaaataat gaaacaataa agcttactgc aactgaatac aaattactca gtctgctggc
    24781 acagcatgaa ggaaaagtat tgacgcacca gtacctgttg cgggaagtat ggggtccggg
    24841 ctatatcagc cagtcacaat atttaagagt gtttatagca caactaagaa aaaaaataga
    24901 aacagatgcc aaccgtccgg aacatatcat caccgaatca ggtgtaggct accggttcat
    24961 cgcaaaggaa taagcattga tacataaaat cattaccatg aaatcgaata gcattcattc
    25021 gaacaggatc actattgcct ctttgcttgt agcactggga atcatttatg gcgatatcgg
    25081 caccagtcca ttatatgttt taaaagccat cgttggcgaa aaacagattg atgaagtact
    25141 ggtgctcggc ggggtctcct gtgttttttg gacattaatg ttgcaaacca cggtaaaata
    25201 tatatggctt actttaaaag cggataatga tggtgaagga ggtatctttt ctttatatgc
    25261 actggtacgc cgttatggga aaaaattagt tattcccgca attcttggtg caactacttt
    25321 gctggcagat ggaataatca ctccgcccat ttccgtcgca tctgcagtag aaggtttgga
    25381 agcagttatt cctaatcttc ctaccgttcc cattgtaatt attatccttt cgcttttatt
    25441 tttctttcag cgtttcggta cacaaaaagt gggtcgcatt tttggcccag caatggtcat
    25501 ctggtttaca atgttatttg ttctcggctt ctcccagatt ctgcattacc cggggatact
    25561 aaaatcacta aatccttatt atgcatacga actactggcc aggtatcctg gtggattttg
    25621 gttactgggt gccgtgttcc tttgtaccac cggcgcagag gcactatact ccgacctggg
    25681 tcactgcggc agaaaaaaca ttcgtcttac ctgggcattt gtaaaaattt gtttactggt
    25741 taattatctc gggcaggctg cctggataat gcaccaggga caacccttgc tggaaggccg
    25801 caatccattt tttgaaatca tgccacactg gttcttgatt gccggtatta tcattgccac
    25861 ggccgcaact ataatcgctt cacaggctct tatcagtggt tcatacacat tgatcagtga
    25921 agcgatgaac ctgaactttt ggccacgggt tagagtccgg caaccctcca atttgaaagg
    25981 gcagatatat attccaagtg tcaatgcaat actttggatt ggttgtgtca tgatgatttt
    26041 atatttccgg agttctaccc acatggaagc agcatatggt ttctccatca ctatcacgat
    26101 gatgatgacg accattttgc tgagttttta tcttatttac aaattaaaat ggaacaaatg
    26161 gctggtggcc ggcattttga ttttgtttgc aactgtagaa acatcctttt tcattgctaa
    26221 cgtagccaaa ataaaagagc ggtggatgtt cctgttcttc gagttgttca tatttatggt
    26281 gatgtatacc tggttttatg caaggaaaat caataataag tttacccggt ttgttgattt
    26341 gggaaaatat gctcccacta taaaagaatt aagcgaagat gataacgttc ctaaattctc
    26401 aacacatctt atttacctga caaaagcaaa taaccgccac caggtagaag aaaaaattat
    26461 caattccatt tttgctaaaa agcccaaacg ggccgatgta tattggtttg tacatatcaa
    26521 ccgtacggaa catccctaca cattgtccta tgatgtatca gaactattgg atgataaagt
    26581 aattaaagta actattaatg ccggcttccg tattcagcca aaaacagagc tttattttaa
    26641 aaaaatagta caggacctgg tgattaaaaa agaactcaac ctgcatatca gaccggatgg
    26701 ctccactaaa tacaatgcac aacctgactt taaattcatt gttatcgaaa aattcctttc
    26761 ggtagaaaat gagtttacag taaaagacgg cttactgctc aattcctatt tcttcctgaa
    26821 agaattgggc caaagcgatg agaaagcatt tggtttggat aaaagtgatg tcgtgatcga
    26881 ggatattccg ttagtatatc aaccatcaca gcatatagaa ttaacaagag taaataacta
    26941 cactaatttt gcaaaatgaa acgggtcctc ttattggttt acctgcatat tgtcactatt
    27001 gctgttaccg ggcaggacag cacaaacaaa aacgacttat ccttttcagg ttttgcagaa
    27061 gtatattacc tgtatgattt cagcaaaccg gcagatggca acaggcctgc ttttaactat
    27121 agccataacc ggcataatga gttcaacctt aacctcggtt ttgcaaaagc aagttttgca
    27181 actgagagag tgaaggctaa tcttgctgtt gctgccggca catatataaa tgcaaactat
    27241 gcagctgagc ccggtgtact gaaaaacatt tttgaagcta atgctggttt caaactcagc
    27301 aaaaagaaaa acctttggct ggatgcaggt attcttcctt cgcatattgg ttttgaaagt
    27361 gctgtcagta aagattgctg gacattaaca agaagcatac tggcggaaaa ttccccttat
    27421 ttcgaagccg gagcaaagct gacatacact actgatgatg gtaaatggat actgagcggg
    27481 atggcgctga acggatggca acgcattaca agactaagtg gcaattcatt gatgagctgg
    27541 ggtacacaaa tacaattcaa gtattctgaa aaagtcttac tgaactacag cacatttatt
    27601 ggtactgata aacctgatag tgcaaggttg tcaaggtttt ttcataatat ctatggaata
    27661 tttaccatta ctaataagat cgggttgata cttggttttg atatcggtac agaagaaaaa
    27721 agtcccggtg caaacagcag taatacctgg tatgccccgg ttgggatttt aaaatatact
    27781 atcaataaca aatgggccat tgccggcaga atagagtatt ataatgataa gaatggcgta
    27841 atcattcctg ctggtaatgc aaatggcttt caaacaacag gttattcgtt gaatatagat
    27901 tatgcaccgg tgaaaaatgt agtgatgcgc ctggaaggga gaacattgaa cagcatggat
    27961 gatattttta gcaatgggag tgcctttaca aaaaacaata cactggtttc gtcttcgata
    28021 gccattaatt tttaaccaaa ggattgagga tttatcacct cgctatcacc ctcattccac
    28081 cacctcctgt attattcaat cctgttttac tcaaactgta agtaaagctg agcaggaaaa
    28141 actggcgcag cgtcagcacc cttgaatctt caatatagtt attattggta ttgcggctga
    28201 tgcccacgtt cctgttcaac aggtccctgg cactgaattt cagttcgccg cggttgaact
    28261 tcagcatttg cttgctgata ctggcattcc acaatggcac ttttatatta aagcccgctg
    28321 cccgctggct gttgatcgta taattaaaat cggtagcgaa gaaaaaacct ttgggcattt
    28381 cccaatccag cgatgtagta tattcctgcg aaagataatt attgctgaag cctggttgca
    28441 atgagtattt cgtgttgtta taactgatac cggcacccaa cccgatattc agttttggtg
    28501 ttgggtttat atccagtctc agatcaggcc ccagtgtcag tgtcttaata gtatttaccg
    28561 ccatgtcccc g
//
LOCUS       DAORTI010000033        28565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1203_length_28565_cov_6.094774, whole genome shotgun sequence.
ACCESSION   DAORTI010000033 DAORTI010000000
VERSION     DAORTI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28565
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1203_length_28565_cov_6.094774"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2294
                     /locus_tag="PLO70_06300"
     CDS             <1..2294
                     /locus_tag="PLO70_06300"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQZ74106.1"
                     /translation="EGIIMLVSIFIVAGISLFQEYRSRNAIQALKKISATKATVIRNA
                     IVNKVPAEDIVVGDILLLEEGEVVAADALIEVANDFTLNESLLTGESFAVTKVADNNN
                     SVYKGTLVTSGSATVRVTAVGLQTMFGKIGLSLKEITVIKTPLQQQIRSFVRKMVWVG
                     VIAFLIIVGFNYSQSGNLLQSFLQGLTLAMSILPEEIPVAFSTFQALGAFRLLRNNII
                     VKQPQYVETLGSATVICTDKTGTLTQNKMSIEYVYDAVSGKSVHVDQKAAIPDLLMEY
                     AMWSSETNPFDPMEKAIHSLYESSCVTDKRSSFKQVHEYPVGGKPPMMTHVFKNDTGE
                     TIIAAKGAPEAILRQSNLSKDESVKYEKQSVDYATQGFRVLGVGKGIWKETHWPVSQE
                     EFSFEFLGLIVFQDPPKENIIQTIKTFNDAGISVKMITGDYAETALAIAGQIHLAHNN
                     EVLTGKDVLHLSKEVLQQRVKQVDIYARMFPEAKLKVIEALKDAGEVVAMTGDGVNDG
                     PALKAAHIGIAMGERGSEVAKNAASLILTDDDLAHMTDAVALGRKIYDNLKKAIQYIV
                     SIHIPIILIVALPLLLWNFTDIFSPVHVIFLELIMGPTCSIIYENEPMEPGTMLRPPR
                     KMGATFLSLKQLAVSIIQGLVITSGCLGIGHYYLQQGSSDDTVRTVIFITLLFSNIFL
                     TLVNRSFYYSVLKTLTYKNKLIPLIILVTLFFIVVVLYVPFVRDLFRLDTLTFQSILA
                     CIVVAIASTFWLEIWKYFVRLRK"
     gene            2451..3446
                     /locus_tag="PLO70_06305"
     CDS             2451..3446
                     /locus_tag="PLO70_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ74107.1"
                     /translation="MSMLKVSGISKAGKENILVSNISFTQQPLQNIAIVGETGSGKTT
                     LLKMIAGLVQPTSGEIIFDNKKVTGPYDQLIPGHPGIAYLSQYFELRNNYWVHEVLEM
                     ANKLSDREANTIFSVCQVNHLLGRRTDELSGGEKQRIALARVLISSPKLLLLDEPFSN
                     LDAMHKSTIKTVIHDIGIKLNISCILVSHEAADTLSWADLILVLKDGQLIQQGAPEQV
                     YKQPVNEYCAGLFGDYNLVDSKTASVLGANSEMIEMNQPLLIRPEFIHITAALSSESN
                     GKVQKRMFWGSCYTIDVFVGQQMIRVKSLRDKFSEGDSVYLSVQNADCWFIPATK"
     gene            complement(3495..3941)
                     /locus_tag="PLO70_06310"
     CDS             complement(3495..3941)
                     /locus_tag="PLO70_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQZ74108.1"
                     /translation="MAETWNKKEREKKKQQNKKEKAERKQERKDNAKPGMGLDDMLAY
                     LDENGNLSSKPPDPRKKVVINVEDIEIGVRKQEPVNPEDLIRKGTVTFFNDAKGYGFI
                     KDDVTGESVFVHINSLEEEIKEHNKVSFEIQMGPKGANAVNVKLLK"
     gene            4235..4594
                     /locus_tag="PLO70_06315"
     CDS             4235..4594
                     /locus_tag="PLO70_06315"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HQZ74109.1"
                     /translation="MKSAFAILFFSFIFLKSSGQNRDLKTALPDSTKKIQVVDAACGQ
                     CQLGLKANGCDLAVRIKGKSYFVDGTSINDHGDAHAEDGFCNSIRKAEIQGEISGNRF
                     KVTYFRLLKKPENKGPK"
     gene            complement(4645..5967)
                     /locus_tag="PLO70_06320"
     CDS             complement(4645..5967)
                     /locus_tag="PLO70_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ74110.1"
                     /translation="MIKAAFVCLLFLTGFCSNSFSQTVYHIRFSFGDSTQGVNEAFLV
                     RYEDGTGFIRATDPFAKDVVVEMEFEELYYIDSLGNIDSNILVIEGFDTKVIRGNSES
                     DYPPLSFWFDKDVETGYFEPATVTSLSIEGTDSTLIFGDFTANLITEEELTEDFVLHF
                     FNKDDDFYQNLFTAVTRVLTTEEKKTRMHLLIVANTEDEKIGSTCVKDKDRMEKTFTD
                     LAEYLGIQLSTKIVYGKEYSKANVEAAITSIKPSPKDIVIFYYTGHGFSKMDNYQFPH
                     LELRSKAYQSLEGNSLNMEEIFSNIKRKGALLNLVISDCCNTEVGAINTISGEHAVTK
                     SSSLGWSLETCKALFLPPKPVSILMTAAAKGEMSAGNNDYGGFFTYHLRASLENYLSP
                     FYQQSDITWAKIIEEAKKQTITKANRTWCLTPDAPKAKCVQHPVYKIQ"
     gene            6199..7575
                     /locus_tag="PLO70_06325"
     CDS             6199..7575
                     /locus_tag="PLO70_06325"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74111.1"
                     /translation="MKKYSLLLIAVSSFFILGAQKQKVDSLSALLAMEKTDTMQVTLM
                     WKLASAFNFFNPDTALIVAQKALYKAREIKYTEGESRALGVVANTFIKIGNYPRALEY
                     HLQKLKLEENRNNPRNMASVLLNIGVVHIFQEDYRQALFYYSKADSIIKNRDVEDLKY
                     YSLLNLGDVYDRLNINDSAFVYFNESLELAKQLADGDFIGSSMVGLGHIYQKQDLFNL
                     AKLNYKNAIPYLETANDDDLICEATLGLAKLYKKMGINDSAELYARHSLTIAEKDGFQ
                     SRHLDAAAFLTEHYKDVRNVDSAFAYMEQSRVLQDSINSRDKIREWQIISSNEQLRQL
                     ELAESRRKASKERSQQLQLLLIGIFIPTFIFFVLLLSRRKIHVRLIRFLGVISLLILF
                     EYLTLLLHPYVAEITHHTPVYELIIFVCIAALLIPTHHRIEHWLIEKLTCRRHHIEAL
                     HKAHPKEK"
     gene            complement(7630..7845)
                     /locus_tag="PLO70_06330"
     CDS             complement(7630..7845)
                     /locus_tag="PLO70_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74112.1"
                     /translation="MLKKKSLPGLAKLLGCLMLASFVITGCNNEGEKKDTGADSATKT
                     EVAPPPPPAIDTSGMDSASTRPVKTPD"
     gene            8037..9887
                     /locus_tag="PLO70_06335"
     CDS             8037..9887
                     /locus_tag="PLO70_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HQZ74113.1"
                     /translation="MLSKKTFLSALFSFIWIPIFAQVERIEPKNWWVGMKDQSLQLMV
                     YGDKIGETTPSINYAGVTIKKVHRAESKNYLFIDLIISSSAKPATFPITFKKEGKTIF
                     TQSYSLLPRKKNTAQLKGFTPADVIYLITPDRFANGDTANDVISSLREKKIDRKNDYG
                     RHGGDIRGIINNLDYIAAMGYTAIWPTPMLENDMPNSSYHGYAITDYYKVDPRYGTLA
                     EYKELADKARQKGLKLIFDGVVNHSGSFYWWMKDLPFRNWINYPDSIQITNHRRTVNQ
                     DPYASTADKELMVKGWFVRQMPDLNQQNPFLSAFLIQNSIWWIETLELNGIRQDTYPY
                     SEKKFLEQWTCKIMNEYPAFSIVGEEWSTNPSIAAYWQQGKLNNSGYNGCLKSTMDFP
                     MQATLVQALTETEAWDKGLVKLYEGLANDFVYANPENLLVFGDNHDMDRLFTFLKKDV
                     DLMQMALSYLLTIRGIPQIYYGTEVLLENSAKPGDHGLIRSDFPGGWKEDGTNGFTGA
                     GLTREQLRIQSFLRKLLNWRKNKTVIHQGNTKHFGPAKGVYVYFRYNKSETVMVVMNK
                     NTGVTMLDTNRFTEMINGKSQATDVVSGNTFSFGNEISVPAKSVLILELK"
     gene            complement(9987..11276)
                     /locus_tag="PLO70_06340"
     CDS             complement(9987..11276)
                     /locus_tag="PLO70_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQZ74114.1"
                     /translation="MKKIFSTFLLGFLPIFVLAQAGHIMQGIGAANISMGGAATAQPI
                     DINGALQWNPAGISAFNTRIFSANAGLFFSSPELSSTVPTSGGPMTGVTKDDRGVSIM
                     PALAMVWGKNNSKHTFGISAFGISGFGVTFPESTTNPINMPQSSGGFGRIESDYQLMQ
                     VAVTYAYKVTDQFSIGFAPTFNYSALELAPNPLSSPSMTKGYPVSDKASALGFGGQFG
                     IFYNSGKGIKLGASYKTEQSFKEFDFENKYLDGSTAPNVKFKMNYPAILSAGIGYSKE
                     NIDFALDYRFVNYENTDGFAEKGWTPTASVAGFGWKNISVISAGLQYKGVKKLPLRVG
                     YTYSSNPISNELAFFSTPATAIIKNAFQLGFGYEFTNHFTLNAAYHHGSSGGSTSGLL
                     LSPMMISSSNPYGAIPGSNVSYKMSTDLVMVGINYSF"
     gene            11637..12071
                     /locus_tag="PLO70_06345"
     CDS             11637..12071
                     /locus_tag="PLO70_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74115.1"
                     /translation="MKHEYWINIKHVDNRMVIFLNGETLWDSGIIHDDPALNHFIEIT
                     DQLLAHPEHASELIFEGFNDTYTSNGSEGDLNPWHFSYRVFKRTVNGTGKVESETDIV
                     IPYNEKHLSNPNIRAINNSYLIVQKNKEFKVVSNSLSQHFYK"
     gene            12283..13206
                     /locus_tag="PLO70_06350"
     CDS             12283..13206
                     /locus_tag="PLO70_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ74116.1"
                     /translation="MQTILGANGTIGSLLAKELTAYSDRIRLVSRNPKKVNETDELFP
                     ADLTDISQLDKAVKGSDVVYLLVGFDYKLKVWQEKWPPLMRATIDACIKHNAKLVFFD
                     NMYLYDKTALSHMTESSAINPPSEKGKVRRAIAEMLMDDVKAGHLKALIARSADFYGP
                     GNDKSFLTEVVFKNFMKGKRSNWFMSADKIHSFTYTPDAAKATALLGNTENAYNQVWH
                     LPTDAAKLTGREIISLFATKMNVKNKVFVIPMWLLKGMGFFVPLLREMPEMMYQYDRD
                     YFFDSSKFENTFHFRATSYTEGVREIVENGR"
     gene            13367..14368
                     /locus_tag="PLO70_06355"
     CDS             13367..14368
                     /locus_tag="PLO70_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74117.1"
                     /translation="MLFVAGFLLCAIIIFFAGKKLSRYGDMLSEKTGLSKGWVGLILM
                     ASVTSLPELMVGISSSAIVQSADLAVGDILGSCAFNLGILAMLDAFVPKRRPLLGVAS
                     PSHMLNAGLGIILLTLVGFAFFLSDYALTPWLAVSSLTFVVVYLIAIRIIYQNEKNHK
                     GEGDNNNFISESVQNTSLRKIILNYSVFAGIIIVAALSLPYFADHIAELTGLGKSFVG
                     TFFLAASTSLPEIAVSIAAVRIGSVDLSVGNLLGSNIFNILILALDDMVYTKGFILQD
                     ASDFHLLSVLSTIVMSAIVIIGLSYRAKGKRFWLAWDAAIIFIVYIINLILLYRFTS"
     gene            14374..16959
                     /locus_tag="PLO70_06360"
     CDS             14374..16959
                     /locus_tag="PLO70_06360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQZ74118.1"
                     /translation="MNWHCLTSKEIFNLTGSSMNGLTREKAALNLAEFGLNELQEKKK
                     KPAWLLFLAQFSDFMILVLLAAAIIAGIAGDITDTIIILIIVVLNAIVGFVQEYKAEK
                     AMEALKKIAAFQAQVIRNGETFTISAAELVPGDLVLLEAGNVVSADIRLIETHSLRID
                     EAALTGESVPAEKTDKVLTEEDLPIGDRLNLAFKSTLITNGRAKGVVIATGMLTEIGL
                     IAKMLQQSESVTPLQKRMSDFGKKLSYIILLICILLFVVGLLRGEKPLNMLLISISLA
                     VAAIPEALPALITIALARGASRLVRKNALVRKLPAVETLGSVTYICSDKTGTLTQNKM
                     KVVEAEVAPGGLKLLDNIPLLYCSIALNNDVKPTGENGLLGDPTEIALVNYFNEKYSS
                     ETLLKLQEQLPRVAELPFDSDRKCMTTVHRYGQQFLVVSKGAIESITESLSDQVNTSG
                     IHKKANGWANNGIRVIGFGYKIVEELPEPFSYESVEKDLLFAGLAGMIDPPREEVKQA
                     IEECKTAGIHPVMITGDHPATAAAIARQIGILTDKDLLITGKELQQLDFDVFESKVEK
                     IRVYARVSPEQKLKIVKALQAKNHFVSMTGDGVNDAPSLKSANIGVAMGITGTDVSKE
                     AADMILLDDNFTTIVKAVKEGRRIYDNIRKFVKYIMTCNGAEIWTLFMAPLLGLPIPL
                     LPIHILWINLVTDGLPGLALAGEKAERDIMKRPPRKTNESLFAEGIGYHILWVGLLMA
                     GVTLGTQAWALQNGDTHWQTMVFTVLSLSQLGHVLAIRSDRVFLYKQGLFSNLPMLGA
                     ILLTFLLQLAVIYLPFANSVLKTQPLTFNELVICLAVSAIVFHAVELEKWIKYKLKVR
                     NQVSL"
     gene            complement(16937..18826)
                     /locus_tag="PLO70_06365"
     CDS             complement(16937..18826)
                     /locus_tag="PLO70_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HQZ74119.1"
                     /translation="MDKNSLFFQAFVYLSAAVVMVPLAKRIGLGSVLGYLLAGIIIGP
                     ALLNLIGSEGHDLMHFAEFGVVMMLFLIGLELEPELLWKLRKSILGLGGLQVLITTII
                     ITAISQSFHIPWQQGLALGMIIALSSTAIVLQTLNEKGLMKTSAGRHSFSVLLFQDIA
                     VIPMLAFFPLLATLQPLTGGGALGKNTWMASLSGWMQTLIVLAAVFAIIFSGRYLINP
                     LLRIVAGTRLREIFTASALLLVIAITLLMTQVGLSPALGTFLAGVVLANSEFRHELES
                     DIEPFKGLLLGLFFIAVGASIDFQLVMQKPVLILGLVFLIMTIKGIVLLILGKLFKLR
                     FDQNLIFGFALSQMGEFGFVLLSFSLQEGVLPKDTIDIMVAVVAISMAVTPLVILVNE
                     KIIQPRFGTKEKENEREDDKIDEKNPVIIAGFGHFGNTIGRFLRANGVQATFLDYDSD
                     RVDLLRKMGFKVYYGDASRHDLLRAAGADQAKIIVLALDNLEKRLEMIDTIKKHFPNL
                     RILVRAENRFDAYQLMNAGMMYVYRETLDTSLRVGTDVLKMMGYRSYTVNRMARIFLK
                     HDEATIKKLAVIKNQDEYVTSAKEYIAEIEKVLESDRHDPELQNDKGWDEESLIADAT
                     GKPGS"
     gene            complement(18830..19438)
                     /locus_tag="PLO70_06370"
     CDS             complement(18830..19438)
                     /locus_tag="PLO70_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQZ74120.1"
                     /translation="MAKLLILFAHPVFEKSRIHKRMVNHTRHLEHVYFHDLYETYPDL
                     DIDINREQQLLLEHDIIIWQHPFYWYSAPAMIKQWQDLVLEHNWAYGANGKMLTGKRI
                     FNALSCGGSLEAYSQTGRNRFSIRQFLTPFEQTATLCNMHYMPPFVVHGTHKMSEADI
                     ELHAVQYLQLLISLVHNRISEDEYQSVNYLNDLLPVPEQVKS"
     gene            complement(19771..20061)
                     /locus_tag="PLO70_06375"
     CDS             complement(19771..20061)
                     /locus_tag="PLO70_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HQZ74121.1"
                     /translation="MVQKTYYKTKDYCKVKFSFKVENAETIEILGLNSDWESSIIMSK
                     KKDGTFSADVNLPKDSKHEFKYLVNETEWINDPEADKQEPNIYGGTNSVVIL"
     gene            20351..20602
                     /locus_tag="PLO70_06380"
     CDS             20351..20602
                     /locus_tag="PLO70_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74122.1"
                     /translation="MNHPYCNDQAYIAESFLIDEDYILQTSRLATFKINSYKLSLIKA
                     AFCKTREEIYNNLQSSLFNYKDPTILLADLGFFEEEIDN"
     gene            20803..22071
                     /locus_tag="PLO70_06385"
     CDS             20803..22071
                     /locus_tag="PLO70_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQZ74123.1"
                     /translation="MKVSLRLRRTTIVLFIIIFLGFILAQCVNRQGEKKTSGNDNATA
                     ETTSSVSYSLFSGSAKCAGCHKDIYDKHIQTAHYLTSQPAEEKYISGSFKPGNNTYSY
                     NPLLRIDMEKRDSGFYQVVYYKGEKKKELRFDIVTGSGVMGQSYLYWRNNKLFQMPVS
                     YFTAAQRWSNSPGFPDKVMLDRPITARCLECHVTYAGISSAPGVEPEEYHHDQIIFGV
                     DCEKCHGPAADHVAFHTENTRDTMAKYIINSSSLSRQQNLDMCVLCHGGNIQKTKPSF
                     TYTPGRSLADYFKIDTLSMVAVQNENIDVHGNQFGLLRSSKCFQQSTTMTCNTCHNPH
                     EKERGKTAIFSQRCMSCHTPGHDNFCKLKLSLTQLSKNCIDCHMPARKSMAVAVSLPG
                     EEVPRAAFVRSHFISIYPDETKKMIENINK"
     gene            22214..22957
                     /locus_tag="PLO70_06390"
     CDS             22214..22957
                     /locus_tag="PLO70_06390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQZ74124.1"
                     /translation="MLIILLFFVAHWYLSLFMQTFLQHRFAAHAAFTMNKFWERFFYG
                     LAYISQGSSYVSPRVYAIMHRMHHAYTDTDKDPHSPTFDKNLFVMMWRVGRLSNGIYN
                     QKVELEPRFLKNLPDWPALDKIGHSVWSRLGWIAVYVMFYVQFAPSAWWYLLIPFHIM
                     MVPFHGMIINWFAHKYGSVNFNLKNTSTNLLKVDWLMMGEGYHNNHHKNPSSTNFGFR
                     WYELDPTYVIIRIFSRLKIIKIHKPVQAL"
     gene            complement(23033..23302)
                     /locus_tag="PLO70_06395"
     CDS             complement(23033..23302)
                     /locus_tag="PLO70_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQZ74125.1"
                     /translation="MNMYVSNLSFHTGDDDLKKLFEQFGAVSSAKVINDRETGRSRGF
                     GFVEMGSDEEANQAIKGLNGKDIDGRSMSVSVAKEKPARSDNKRW"
     gene            complement(23561..25012)
                     /locus_tag="PLO70_06400"
     CDS             complement(23561..25012)
                     /locus_tag="PLO70_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ74126.1"
                     /translation="MEITAQPISQNRSKLSAVKLNGYFLRLKKQYQVLLRFVKAIGFT
                     ETMEEYDQRKLGIFNKLNFFQLITGILIPVGGMFSNSQLPVSIWIIACLPALVSFIVL
                     FFNYQRKHETALLVYFILYPFFTCVVYLYGFNPGVGLTFILFGILSVFFLKDTGYIIF
                     CLCFSMVSYFLLAVVIEHQPYELQEFNNGLYLFNQGLALVFIFYGLFLIKKENDIYHT
                     RILSKNYMLEGKNEQIQKQTNKLEESAELLKQQATQLAELNTVKTKLFSIISHDLKAP
                     MYAIRNLFREANEKNMSAEELKNNIPEVLKDMNYAVGLMENLLQWAKTQMQSTTVNPE
                     VVDIEKSIDEVYQSLHLQAKNKGIIMLNDAPPRVHGFIDKDMMNLVLRNLLSNAIKFT
                     PEKGTISIGVHEHPSFIEIYVKDSGMGMSHEALAKIRNNDYFTTKGTASESGTGLGLM
                     LCKEFLVKNGGQLYIESQPGEGSTFSFSVPKPF"
     gene            25408..26502
                     /locus_tag="PLO70_06405"
     CDS             25408..26502
                     /locus_tag="PLO70_06405"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ74127.1"
                     /translation="MKIQPAIRYKVRQVINITVLWLIIGVLVEIHNAINYNPEKGSFF
                     IYFIFGGNALQHLLITSLGPVAGGLLAGSFIVFYQREKMKGKTYGQKLFIHSLLYLIF
                     VSLCILIVGTIGAINDSAAASFWEKFDAAVFNLRVLRLLITWYFIVLFTIFFLDISEK
                     YGPGTLKKMLLGRYHSPVQEDRIFMFLDLKSSTTIAEKIGDEFYFRMLKYFFQLATEP
                     VLNCKGEIYQYVGDEIIISWNRKEGLQNANCLQCFFSIQETIRAKSDYFKTQFGTVPD
                     FKAAIHSGIVTTGEVGVIKKDIVYSGDILNTTARIMSLCNQYNTSLIISGVIYEELKR
                     TAGYEFNWLDSPGLRGKTTKTPVFSVNRKV"
     gene            26595..28523
                     /locus_tag="PLO70_06410"
     CDS             26595..28523
                     /locus_tag="PLO70_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ74128.1"
                     /translation="MAEHSHQLAAILFADIVGYTAMMQENEEVAVEKINRFREVVELI
                     AEELNGKIIQYYGDGSLLLFHSSTDAAEFAKLLQEDLQNEPRIPVRIGIHMGDVMVHS
                     GNIFGDVVNIASRIQQLAPVGGIYISEMVYRNIANKKELESVFIREEKLKNVKIPIRI
                     YEIVTAGSQPFTQQAPAAEPVLPSTNANSIAVLPFSNLSSDPQQDYFGDGLTEDIITQ
                     LSKIRAYKVISRTSVMQYKKSPKSIIEIGKDLGVALILEGSVQRHGEQVRITAQLINA
                     ITDEHIWAESYDRSMNDIFSIQREVALAIAAVLNTKLSNRETRELDYIPTSDLQAYDL
                     YLRGKLLMENRNKADLLTARDLFTQAVNRDKTFAIAYSGLADTYLLSSFRGYEDPVMM
                     LWQAKKYIDTAAGLDSFSGETQASLGYWYFQSFDWHAAELTYRRSISLNANQSNVYLW
                     LAILLQAKGENEEALKVYDLGTEVNPMWDFLLKNKIIALVNMNNKEAALELQHTLIER
                     ASHDSHLKRKRYEELSRLYWSFNDKEEAITAAKKSGDLALIKFYSDGDHSLLEKRQDD
                     HYSALRNRSEYISQFWMGLDYAHAGAREKALECFNNAIALKEAGISLLLIRDFEFLNI
                     KYLSMALITRKVKQLINF"
BASE COUNT         8590 a         5200 c         5863 g         8912 t
ORIGIN      
        1 aggaagggat tataatgctg gtatcgattt tcattgtggc gggtatctcc cttttccagg
       61 aataccggag ccggaatgca atacaagctt taaaaaaaat atcagccaca aaagcaactg
      121 tcatccgaaa tgctattgtt aataaagttc cggctgaaga tattgtagtg ggtgatatcc
      181 tgctgttgga ggaaggggaa gtggtcgcag ctgatgcctt gattgaagtg gccaatgatt
      241 ttacacttaa tgagtcttta cttaccggtg aatcatttgc agtaacaaaa gttgcagata
      301 ataataatag cgtttataag ggaacattag tgaccagtgg aagtgcgaca gtgagggtaa
      361 cagcggtggg tttacagacc atgtttggga agattggatt gtctctgaaa gaaattacag
      421 ttataaaaac accattacaa cagcagatac gttcatttgt ccggaaaatg gtgtgggtgg
      481 gtgtaattgc ttttctgatt attgtcgggt tcaattattc acaatccggt aacctgttgc
      541 aatcgttttt gcagggatta acattggcca tgagtattct gcctgaagag atacctgttg
      601 ctttcagcac ttttcaggca ttaggtgctt ttcggttatt acgtaacaat attattgtaa
      661 agcaaccaca gtatgtggaa accctgggct ctgctacagt aatctgtacg gataagactg
      721 gtactcttac tcaaaataaa atgagtattg agtatgtcta tgatgctgtt tcgggaaaat
      781 cggttcatgt agatcaaaaa gccgctattc cggatttgct aatggagtat gcaatgtgga
      841 gcagcgaaac caatcctttc gaccccatgg aaaaagctat ccattcttta tatgaaagta
      901 gttgtgtcac tgacaaaaga tcttcgttta aacaggtgca tgagtacccg gtaggtggca
      961 aaccgcccat gatgactcat gtttttaaga atgatacggg tgaaacaatc atagctgcta
     1021 aaggagcgcc ggaggctatc ctgagacaaa gcaatttgtc aaaagatgaa tctgttaaat
     1081 atgaaaagca atcagtagac tatgcaacac aggggtttcg tgtgctgggt gtgggaaaag
     1141 gaatttggaa agaaacacac tggcctgttt cgcaggaaga atttagcttt gaatttctgg
     1201 gattgatcgt atttcaggac ccacccaaag aaaatataat tcaaacgatc aaaacgttta
     1261 atgatgccgg tatctcggtt aaaatgataa ctggtgatta tgcagaaacg gccctggcta
     1321 ttgctggcca gatccatttg gcacataata atgaagtgct aacgggtaaa gatgtcttgc
     1381 atctttcgaa ggaagtattg caacaaaggg tgaaacaggt ggatatttat gccaggatgt
     1441 ttcctgaggc aaagctgaag gtaattgaag cattaaaaga tgctggtgaa gtagtagcca
     1501 tgactggtga tggagtaaat gacgggcctg cattaaaagc cgcacatatc ggtatagcga
     1561 tgggggaaag gggaagcgag gtagctaaga atgcggcttc gttaattctt actgatgatg
     1621 atctggcgca tatgacagat gcagtagcgt taggaaggaa aatctatgat aatctcaaaa
     1681 aagccattca atatatagta tctattcata tacctattat tcttattgtg gcccttccgc
     1741 tgctgctgtg gaatttcact gatatctttt caccggtaca tgtaattttc cttgagttga
     1801 ttatggggcc aacctgttca atcatttacg agaatgaacc aatggagccg ggcacaatgc
     1861 tacggccgcc caggaaaatg ggggctacat ttttatcttt gaagcagctg gctgtcagca
     1921 ttatccaggg gttggtcatc acctccggtt gtttgggtat tggccattat tatttacagc
     1981 aggggagtag tgacgacact gtccgcacag tgatctttat tactttatta ttcagcaata
     2041 tctttcttac cctggtaaat cgttcatttt attattcggt attaaaaaca ttgacgtata
     2101 aaaacaaatt gatcccatta atcatcctag taacattatt ttttattgtt gtggtcttat
     2161 atgttccttt cgtacgggat ttattccggt tggatacatt gacgtttcaa tccattcttg
     2221 cctgtattgt ggttgccatt gccagtactt tctggctgga gatatggaaa tactttgtac
     2281 gactacgaaa ataatattaa aaattaataa agtgccaggt gaggttttgt tgctgacagg
     2341 taataaatcc gtttatatct tttattacga gtcttaaatt gatcctgcat cactccttcg
     2401 tattttatga ttttacttgt catattaacg ctatgcttta atttcgaagc atgtcgatgt
     2461 taaaagtgtc aggtatcagt aaagcaggga aggaaaatat tttggtaagc aatatcagct
     2521 tcactcaaca accccttcag aatatcgcca ttgtcggtga aacaggatcc gggaaaacaa
     2581 ccctgcttaa aatgatagca ggattggtac aaccaaccag cggggaaata atttttgaca
     2641 ataagaaagt tacgggccct tatgatcagc taataccagg ccatcccggc atagcttatc
     2701 tcagccagta ctttgagttg cgaaataatt attgggtgca tgaagtgctt gaaatggcaa
     2761 ataaattgtc agatagggaa gccaatacaa ttttcagcgt ttgccaggta aatcatttat
     2821 tgggacgtag aacggatgaa ctttccggcg gggaaaagca acgcattgca ttagcaaggg
     2881 tactaatttc ttctcctaag ttattattgc tggatgaacc tttttccaac ctggatgcga
     2941 tgcacaaaag cactatcaaa acggttatac atgacattgg gataaaattg aacattagct
     3001 gtatccttgt ctctcatgaa gctgcagata ccttgtcctg ggctgatctt attctggtac
     3061 taaaagatgg ccagctaatt cagcaaggag ctcctgaaca ggtatataag cagccagtaa
     3121 atgaatattg tgccgggctt ttcggggact ataacctggt ggacagtaaa acggcttcgg
     3181 ttttaggggc aaattcagag atgattgaaa tgaatcaacc tttactcatc cgtccggaat
     3241 ttatccatat tactgctgcc ctcagttctg aatcaaatgg taaagttcaa aaaagaatgt
     3301 tttggggcag ctgttataca attgatgtat ttgttgggca gcaaatgata agagtaaagt
     3361 ctttgagaga caaattttct gaaggagata gcgtatatct atctgtccag aatgctgatt
     3421 gttggtttat accagctaca aagtaacaag ctgatgatat attgggctat atgataggtt
     3481 gcgccagtaa aaaattactt tagcaatttt acatttaccg cattggcgcc ttttggcccc
     3541 atttgaattt caaatgatac tttattatgt tccttgattt cttcttctaa tgaattgatg
     3601 tgtacaaaaa cactttcacc ggttacatca tccttaatga atccatatcc tttcgcatca
     3661 ttgaagaaag taacggttcc tttccggatc aggtcttccg gattaacggg ttcctgtttg
     3721 cgaacaccaa tttcgatgtc ttctacgttg atcaccactt tttttcgggg atctggtggt
     3781 ttggatgaca aattaccgtt ttcatccagg taagcaagca tatcatcaag acccatacca
     3841 ggcttggcat tatcttttct ttcctgcttt ctttccgctt tttctttttt gttttgttgt
     3901 tttttctttt ctctttcctt tttattccag gtttctgcca taatgctttt ctattttatt
     3961 tttaaaagtt ctttttaaga tggggtagaa ttgcaggaaa tacgctgttg aaaaaatgtc
     4021 agattcactg cagattcaga tgaaggtacg ctatttttct aactcagctc cttaagccct
     4081 tatttctgca ctttcccatg attaaaaact gttatacaaa gacgtttatc agcaggttga
     4141 attggtattc ccgggtactg cagttttaat tttttaaata aatcattgtt ttttctatgt
     4201 aggtttacag cattattaat tacaaattct ttttatgaaa tcagcatttg ctatattgtt
     4261 ttttagcttt atttttttga aatcgtcagg gcaaaacagg gacttaaaaa cagctcttcc
     4321 agacagtact aagaaaatac aggtggtgga tgctgcctgt ggacaatgcc agttagggct
     4381 gaaggcaaat ggttgcgacc tggctgtgcg cattaaaggg aaatcatatt ttgttgacgg
     4441 aacatctatt aatgatcatg gggatgctca tgcagaagat gggttctgta attcaatccg
     4501 aaaagctgag attcagggtg aaatttcggg taaccgtttt aaggtcactt atttcaggct
     4561 gctcaaaaaa cctgaaaata agggccccaa ataataataa gaatacctgg ttaacgattt
     4621 agcagaaaat aatatcaccc aattttactg gatcttgtaa accggatgct gcacacattt
     4681 tgcttttggt gcatcaggtg ttaaacacca ggtgcggttg gctttggtta tggtttgctt
     4741 ttttgcttct tcaatgattt tggcccaagt gatatctgat tgctggtaaa aaggactcag
     4801 gtaattttca agcgaagccc tcaggtgata ggtaaaaaaa ccaccataat cattattccc
     4861 ggctgacatt tctccttttg ctgcagctgt cattagtatg gagacaggtt taggaggtaa
     4921 aaaaagtgct ttgcaggttt ctaagctcca tccaagggaa gaactttttg taacggcatg
     4981 ttcaccgctt attgtattaa tagcacctac ttcggtatta cagcaatcac ttatcaccag
     5041 gtttaacagg gctccttttc tttttatgtt actgaatatt tcctccatat tcagagagtt
     5101 gccttccagg ctttggtaag cctttgatct cagttcaagg tgggggaact ggtaattatc
     5161 cattttgctg aatccatgtc cagtgtaata aaaaataaca atatcttttg ggcttggttt
     5221 aattgatgtt attgcagctt ccacatttgc tttactatat tcttttccat atacaatttt
     5281 ggtactaagc tgtataccca ggtattctgc caggtctgta aaggtttttt ccatcctgtc
     5341 tttatctttc acacaggtac tgccgatttt ttcatcttcc gtatttgcca ctatcagcag
     5401 atgcatcctg gttttctttt cctcagttgt aagtactctg gtaacggctg taaagaggtt
     5461 ttgataaaag tcatcatcct tattgaaaaa atggagtaca aaatcttcag tcagttcttc
     5521 ttcggtaatc aggtttgcag taaaatcacc aaatatcaat gtgctatcag taccctcaat
     5581 ggacagtgag gtgacagtcg ctggttcaaa ataaccagtt tctacatcct tgtcaaacca
     5641 gaatgaaagg ggagggtagt cactttctga atttccccgg atcacttttg tatcaaagcc
     5701 ttctataaca agaatgttac tgtcaatatt acccaaagaa tcaatgtagt ataactcttc
     5761 gaattccatt tcaactacta cgtcctttgc aaaagggtca gtagccctta taaaaccggt
     5821 accatcttcg tacctgacta aaaatgcttc attcactccc tgggtgctgt cgccaaaaga
     5881 aaagcggata tgataaactg tctgggaaaa ggaattactg caaaatccgg taagaaacag
     5941 caggcataca aaagctgctt ttatcataac taattagttt tggtgaagac ttctaattta
     6001 tgaatttaag ttattattta ttattgtcaa tttttcttcc ttatttggtt gaaatatggg
     6061 tgacagtaaa agagactctt attttaaatg agtggcaaaa ttatgtttgt gatcgagatt
     6121 ttttaacttc tggcttcagg aggggtttag ttgccccgga ctgaatttct tttaattatt
     6181 taagcataat tttcaggtat gaaaaaatat tcactgctgc ttattgctgt tagcagtttt
     6241 tttattctgg gcgcacaaaa acaaaaagtg gatagcctta gtgctttgct ggcaatggaa
     6301 aagacagata caatgcaggt aacactaatg tggaagcttg ccagtgcctt caattttttt
     6361 aatcctgata cagctttgat cgttgctcaa aaggccctat acaaagccag ggagataaaa
     6421 tatactgaag gagaatcaag ggcattaggt gttgtggcta acacctttat aaagattggt
     6481 aactacccaa gagccctgga atatcatctg cagaaattaa agctggaaga aaacaggaat
     6541 aacccccgaa atatggccag tgtacttttg aacattggtg tggtgcatat tttccaggaa
     6601 gattaccgac aggcactttt ttattattcc aaagctgatt caattattaa gaacagggat
     6661 gttgaagatc ttaaatatta cagcctgcta aatcttggtg atgtatatga tcgtctgaat
     6721 attaacgatt ctgcttttgt ctatttcaat gagtccctgg aactggcaaa gcaactggcg
     6781 gatggcgatt ttattggtag ttcaatggtt ggcctgggac atatttacca gaaacaggat
     6841 ctatttaatt tggcaaagct caattataaa aatgcaatac cctacctgga aacagctaac
     6901 gatgatgacc tgatatgtga ggcaactttg ggtttagcaa aattgtacaa aaaaatgggt
     6961 attaatgatt cagcggagct ttatgcccgt cattcattaa cgatagctga aaaagatggt
     7021 tttcagtcaa ggcacctgga tgcagctgct tttttgactg aacattataa agatgtccgg
     7081 aatgtagaca gtgcctttgc ttatatggag caatcccggg tattgcagga ctctatcaat
     7141 agcagagata agatccgtga atggcagata atttccagca atgaacaatt aagacagctg
     7201 gaactggcag aaagcaggcg aaaagcttcc aaggaaagat cgcagcaatt acaattactg
     7261 ttgattggta ttttcattcc aacctttatc ttttttgtgt tattactcag cagaagaaag
     7321 attcatgtcc ggctgatcag gttcctggga gtcatttcgc tgttgatatt atttgaatat
     7381 ttaaccctgc tgctgcatcc ttatgttgcg gaaattactc atcatacgcc tgtgtatgaa
     7441 ttgatcattt ttgtatgtat cgcggcatta ttgattccaa cccatcatcg tattgaacat
     7501 tggttgattg aaaaattaac ctgcaggagg caccatattg aagctttaca taaagcgcat
     7561 ccaaaagaaa aataataaaa aaacttcctg ttgtacacag gaagtttttt atttataaaa
     7621 gcattcagtt taatcaggag tttttactgg tctggtcgat gcagaatcca tgccgcttgt
     7681 atcaatagcc gggggtggtg gtggagctac ttcagttttt gtagctgagt cagcaccggt
     7741 atcttttttt tcaccttcat tattacatcc ggtaattaca aatgacgcca gcataaggca
     7801 gccgagcaac ttagccaggc caggtaatga ttttttctta agcatattaa ttaggtttta
     7861 ggatgaaaat atacaattaa aaattcaaat atgattttgg acgattgaat tatttttagt
     7921 ggttctaccc cgaacaaccc aaaagagggt aaccgggact gaaaaaatag ctttgcagga
     7981 attcaaatac ctttatcaat aaagctgtat tattttagaa atcgaaaaat gcctgtatgt
     8041 tatcaaaaaa aacattttta tctgctctgt ttagttttat ctggataccc atttttgcac
     8101 aggtggaacg tatagagcca aaaaattggt gggtgggtat gaaagatcaa tccctgcaat
     8161 taatggtgta tggtgataaa ataggtgaaa ccactccctc tatcaattat gccggggtta
     8221 ctattaagaa agttcaccgg gcagaaagta agaactatct ttttattgat ctcataattt
     8281 cttcttctgc caaacctgcc acattcccaa ttacgttcaa aaaagagggt aaaacaattt
     8341 ttacgcaatc gtattcattg ctgccacgga aaaagaatac agctcagtta aaagggttta
     8401 ctcctgcaga tgtaatctat cttattacac ctgacaggtt tgcaaatggt gatacagcta
     8461 atgacgttat aagcagttta cgtgaaaaaa agatcgatcg taaaaatgac tatggacggc
     8521 atgggggtga tatccgtggc ataatcaata atcttgatta tatagcggcc atgggctata
     8581 cagctatctg gccaacgccg atgctggaga atgatatgcc caattcatcc tatcatggct
     8641 atgccatcac tgattactat aaagtagatc cccggtatgg tactttggct gaatacaaag
     8701 agttggccga taaggcaagg caaaaaggac ttaaattgat ctttgacggg gttgtaaacc
     8761 attccggttc tttttattgg tggatgaaag atctgccttt taggaactgg atcaattatc
     8821 ctgattcgat ccaaattacg aatcaccgtc gaacagtgaa ccaggatcct tatgcttcaa
     8881 ctgctgataa ggagttaatg gtgaaaggat ggtttgtaag acagatgccc gacctgaacc
     8941 agcaaaatcc tttcctgtct gcctttttga tccagaatag tatctggtgg attgaaacac
     9001 ttgaattgaa tggtatcagg caggatacct acccgtattc agagaaaaaa ttcctggagc
     9061 aatggacctg taagatcatg aatgaatatc cagctttcag cattgttggg gaggaatgga
     9121 gtaccaatcc atcgattgca gcgtactggc aacaaggaaa attaaataac agtggctata
     9181 acggttgtct gaagagtacc atggattttc cgatgcaggc aacgctggta caggcattga
     9241 ctgaaacaga ggcatgggac aaaggactgg taaaactata tgaagggttg gctaatgatt
     9301 ttgtttatgc gaaccctgag aacttactgg tctttgggga taatcatgat atggacaggt
     9361 tgttcacctt tctgaaaaag gatgtggacc tgatgcagat ggcgttgagc tatctgctta
     9421 cgatcagggg tattccccag atctattatg gaacggaggt cttgctggaa aatagtgcaa
     9481 aaccaggtga tcatggattg atacgttctg attttcccgg gggatggaaa gaggatggaa
     9541 cgaatggatt taccggtgca ggattgacaa gggaacaatt acggattcaa tcttttctca
     9601 gaaaattgct gaactggcga aaaaataaaa cagtcattca ccaggggaat actaaacatt
     9661 ttggtcctgc caaaggagta tatgtttatt tcagatacaa taaaagtgag actgtaatgg
     9721 tagtgatgaa taagaatacc ggtgttacga tgttggacac gaatcgtttt accgaaatga
     9781 ttaacggtaa aagtcaggct accgatgtag ttagtggaaa tactttttct tttggaaatg
     9841 aaatatcagt tcccgcaaaa tctgttttaa tccttgagtt aaaatagtaa tacttatgtt
     9901 tggctaatct gtaaaaataa aggctgactt tttgagtcag cctttataat aaagaacgga
     9961 tagttgttat aagaacaaac tgtttatcag aacgaataat tgattcctac cataactaaa
    10021 tcagtactca ttttataaga tacgttactg ccgggaatgg caccataagg gttagatgaa
    10081 ctaatcatca tagggcttaa taataagcca ctggtagaac caccgctgga gccatgatga
    10141 tatgcagcat tcagtgtgaa atgattagta aattcataac caaacccaag ctgaaaagca
    10201 tttttaataa ttgcagtagc cggagtggag aaaaatgcaa gttcgttgct gattgggttt
    10261 gagctatagg tataaccaac tcttaatgga agttttttta ctcctttata ttgcaatcca
    10321 gcagaaatga cagatatatt tttccatcca aatccagcga ctgatgcagt aggggtccat
    10381 cctttttcag caaagccatc tgtattttca tagtttacaa aacgataatc aagagcgaaa
    10441 tcaatatttt cttttgaata gcctattcct gcagaaagaa tagccgggta attcattttg
    10501 aattttacat tgggagctgt actgccatcg aggtatttat tttcaaaatc aaactcctta
    10561 aaggattgtt ctgttttata agaggctccg agtttaattc ctttacctga gttgtagaat
    10621 ataccaaact ggcctccgaa tcctaatgca gaagctttat cgcttaccgg ataacctttt
    10681 gtcatactag gagaggaaag gggatttggt gccagctcca gtgctgaata gttaaaagtt
    10741 ggtgcaaaac cgatagaaaa ctgatcagtt accttgtatg catacgttac agcaacctgc
    10801 atcaactggt aatcagattc aatacgtcca aacccacctg aactctgagg catattgata
    10861 gggttggtag tactttcagg gaaagtaaca ccaaatccac ttattccaaa agcagatatg
    10921 ccaaaagtat gtttactgtt attcttcccc cataccattg ctaaggctgg cataatggaa
    10981 acacctctgt cgtctttagt tactcctgtc attggtcccc ctgaagttgg aacagtcgat
    11041 gacaattcgg gagatgaaaa gaataaaccg gcattagcag aaaaaattct tgtattaaac
    11101 gctgaaatac ctgcagggtt ccattgtaaa gcaccattga tatcaatcgg ctgtgcagta
    11161 gcggctcctc ccattgatat attagcagct ccgattccct gcataatatg acctgcctgt
    11221 gctagtacga aaatgggcaa gaaacccaga agaaatgttg aaaatatctt tttcatataa
    11281 gtgtgttttg aattattcct gcagtagctg cagaaagaat tgtaaaacta cttttgcatt
    11341 attccggctt ttgtgactga tatcacgtcc gggctttaat catagtgacg tatgattaag
    11401 aacatcattt gaactgaaaa atgttagtga ttaattcata tattattacg aatgctctaa
    11461 aacacaacat gaattagctg aattttcctg ctggtatggc tgaaaaatat cacttgtagt
    11521 aaccagtatc aggtcatagt ataacttttg tcatacccct gttaatggga tacattctat
    11581 tttcgccact gtattttttt taaaaaccca ttctttaaag tttaatattt tctaaaatga
    11641 aacacgaata ctggataaac attaaacatg ttgataaccg catggttatt tttttaaacg
    11701 gagaaacgct ttgggacagt ggcattattc atgatgaccc tgccctgaat cattttattg
    11761 aaatcactga tcagttactg gcgcatccgg agcatgccag tgaattgata tttgaaggtt
    11821 ttaacgatac ttacacttcc aatggttctg aaggtgatct gaacccctgg cattttagtt
    11881 accgggtatt taagaggact gtcaatggta ctggaaaagt tgaaagcgaa actgatattg
    11941 tcattcctta caatgagaaa catctttcaa atcccaatat cagggctatc aataacagct
    12001 acctgattgt acagaagaac aaggaattta aggttgtatc taattcatta tcacaacatt
    12061 tttacaagta agttttcatt atacaggtca aataataatt ttcaatagct attatatata
    12121 ttatttgctg gttgcttctc gataaacaaa aagacggaat tatttattcc gtctttttgt
    12181 ttgttaccgg ttattctgct cttcattttc aattaaagta taaaaatctg tgtgtgaatt
    12241 aatttattgt tttctgtaac ttgtcaaaaa tcagattatc tcatgcaaac aattttaggg
    12301 gctaatggta ccattggttc tcttttggca aaagagctta cggcttattc agatagaata
    12361 cggttggtga gccgtaatcc caaaaaggtg aatgaaacag atgagctgtt tccggcggat
    12421 ctgacagata tcagccaact ggataaagct gtaaaaggtt cagacgttgt ttacctgttg
    12481 gtcgggtttg attacaaact aaaagtatgg caggagaaat ggcctccatt gatgcgggct
    12541 actattgatg catgcataaa gcataatgca aagctggttt tttttgataa tatgtatttg
    12601 tatgataaaa cagcattgtc acatatgact gaaagttccg ctataaatcc gcccagtgaa
    12661 aaaggaaagg taagaagagc gattgcagaa atgcttatgg atgatgttaa ggcgggacac
    12721 ctgaaagcgt taattgcacg gtcagcggat ttttacggac caggtaatga taaaagtttc
    12781 ctgacggaag tggtattcaa aaatttcatg aaagggaaaa ggtcaaactg gttcatgagc
    12841 gcagacaaaa tccattcttt tacctataca ccagatgcgg ctaaagccac tgctttattg
    12901 ggcaatacgg aaaatgccta taaccaggtg tggcatttgc ctactgatgc tgcaaaactt
    12961 accggacgtg aaatcatttc actatttgca acgaaaatga atgtgaaaaa taaggtattt
    13021 gtaattccaa tgtggttact gaaaggaatg ggcttttttg ttcctttatt aagggagatg
    13081 cctgaaatga tgtaccagta tgatagagat tatttcttcg actcatctaa atttgaaaat
    13141 acttttcatt tccgggcaac ctcctatacc gaaggagtca gggaaattgt agaaaatggc
    13201 cggtaattct gtttgccaat ttgcaggaaa gcaggactaa tttgtcaatt attaccctat
    13261 aacctgaata tttaaccagg tgatttttat cactgattca ttgtatctga ctcaccagca
    13321 gagtgctttt ctttatttaa attgcagcta agaaaattca gctgctatgt tatttgttgc
    13381 tggcttttta ttatgtgcta taattatttt ttttgcgggg aagaaattgt cccggtacgg
    13441 ggatatgttg tcagaaaaga cgggcttaag taaaggttgg gtcggtttga tactgatggc
    13501 atctgttact tctttaccag aactgatggt tggtatcagt tcctctgcta ttgttcaatc
    13561 tgctgatctc gctgttggcg atattttggg tagttgtgcc tttaacctgg gtattcttgc
    13621 aatgttggat gcttttgtgc caaaacgccg tccacttctg ggagtggctt ctcccagtca
    13681 tatgctgaat gcaggacttg gaattatact tttgacctta gttggttttg catttttcct
    13741 ttcagattat gctttgacac catggttggc tgtttcaagc ttaacctttg ttgtggttta
    13801 tcttattgca attcggatta tatatcaaaa tgagaaaaat cataaagggg aaggggataa
    13861 taataatttt atttctgaga gtgtacaaaa cacaagcctc cggaaaataa ttcttaatta
    13921 cagtgttttt gctggtatca ttatagttgc tgcattgtct ttgccttatt ttgcagacca
    13981 tattgcagaa ttaacaggat tagggaaatc atttgtagga acattttttt tggcggcatc
    14041 aacttccctt ccggaaatag cagtttctat cgcagcagtt cgaatcggtt ctgtagatct
    14101 ttcagtcggc aatctgctcg gtagtaatat ttttaatata cttatactgg ctcttgatga
    14161 tatggtgtat actaaagggt ttattttaca ggatgcttcc gattttcatt tattatctgt
    14221 tctgagtact attgtcatgt cggctattgt tattatcggc ttaagttacc gggcaaaagg
    14281 taaacgtttc tggctggcat gggatgctgc tattattttt attgtttata taataaacct
    14341 cattctttta tatcgattta cttcataaat gttatgaact ggcattgtct cacttctaaa
    14401 gaaatattca acctgacagg ttcctcaatg aatggattaa cccgggagaa agctgcattg
    14461 aacctagcgg aatttggact gaatgagtta caggagaaaa agaaaaaacc ggcgtggtta
    14521 ctttttctgg cacaattcag tgattttatg atcctggtat tattggctgc cgctatcatt
    14581 gcaggaattg caggagatat taccgatact attattatac ttataatagt cgtactgaat
    14641 gcaattgtcg gatttgtgca ggagtataag gcagaaaagg caatggaggc cctgaaaaaa
    14701 attgccgctt ttcaggcaca ggtcatacgt aatggggaaa ctttcacaat aagcgctgcg
    14761 gaacttgtgc ccggtgacct ggttttactg gaggcaggta atgtagtatc cgctgatata
    14821 cgactgatag aaacacatag tttgcgtatt gatgaggctg ctttaacagg ggaatctgta
    14881 cctgctgaaa aaacagataa agtacttaca gaagaagatt taccaattgg tgacaggctt
    14941 aacctggcat ttaaaagcac attaattact aatgggcggg caaaaggtgt tgttatcgct
    15001 actggtatgc taactgaaat aggactgatt gcaaaaatgc tacagcaatc agaatctgta
    15061 acaccgcttc aaaaaaggat gagtgatttc ggtaaaaagc tatcctatat tattttattg
    15121 atatgtattt tgttatttgt agtaggtctg ctaagaggcg agaagccact gaacatgtta
    15181 ctaatatcga tttcactggc ggtagcagct attcccgaag ccttgcctgc tcttattaca
    15241 attgcattgg caagaggcgc cagccggttg gtcaggaaga atgctttggt acgaaaactc
    15301 ccggctgttg aaacactggg ctcggtcacc tatatctgtt ctgataaaac cgggaccctg
    15361 acacaaaata aaatgaaagt ggtggaagca gaggtggctc ctggtgggtt aaagcttctc
    15421 gataatatcc ctttattata ttgttcaatt gcattaaata atgatgtaaa accaaccggt
    15481 gaaaatggtt tgctgggtga tcccactgaa attgcattag ttaattactt taatgaaaaa
    15541 tattcatcgg aaaccctgct caaattacaa gaacaattgc caagggtagc cgagttgcca
    15601 tttgattctg ataggaagtg tatgacaact gtacatcggt acgggcaaca atttttagtg
    15661 gtttctaaag gagcaattga atccataacc gaaagcttat cggaccaggt taacacttca
    15721 ggtattcata aaaaggcaaa tggctgggca aacaatggta tccgtgtgat tggattcggt
    15781 tacaaaatag tagaggagtt gcctgagcca tttagttatg agagtgttga aaaggattta
    15841 ttatttgcag gattagccgg catgatcgat ccgccccggg aagaagtaaa acaggcaatt
    15901 gaagaatgta aaacagccgg aatacacccg gtaatgataa ccggtgatca cccggctact
    15961 gctgccgcaa ttgccagaca gatagggatt ttaactgata aagaccttct tatcacaggt
    16021 aaggaattac aacaactaga ttttgatgtg tttgagagca aggttgaaaa gataagggtt
    16081 tatgccaggg tttcgccaga acagaaattg aagattgtaa aagctttgca ggcaaaaaat
    16141 cattttgttt ctatgacagg tgatggggtg aatgatgcgc cttcgttaaa atcagccaat
    16201 atcggtgttg caatgggtat tacgggtact gatgtcagta aagaagcggc cgacatgata
    16261 ttgttggatg ataatttcac aacaatagta aaagcagtga aagaaggcag gcggatatac
    16321 gataacatcc gcaagtttgt taagtatatc atgacctgca atggtgccga gatatggaca
    16381 cttttcatgg cgccattact cggattaccc attcctttgt taccgataca tattctatgg
    16441 atcaatcttg ttactgatgg cttacctggt cttgctctgg ccggagagaa agctgaaaga
    16501 gatatcatga aaaggcctcc aagaaaaaca aatgaaagtc tttttgcgga aggtataggt
    16561 tatcacattt tatgggttgg tttgctgatg gctggtgtta cgctgggtac acaggcatgg
    16621 gctttgcaaa atggtgatac ccactggcaa acgatggtat ttacagtttt atctctctct
    16681 cagctgggac atgtgctggc catccgaagt gacagggtct ttctttacaa gcagggttta
    16741 ttcagtaacc tgccaatgct tggcgctatt cttcttacat ttttattaca gttggctgtt
    16801 atttatctgc cttttgcaaa ttcggtacta aaaacacaac cattaacatt taatgaactg
    16861 gtgatctgtt tagcagtttc tgctattgta tttcatgctg tagaactgga gaaatggata
    16921 aagtataagc taaaggtcag gaaccaggtt tccctgtagc atcagctatt aatgattcct
    16981 catcccagcc tttatcgttt tgcaattcag gatcatgcct gtcagattca agtacttttt
    17041 caatttctgc tatgtattct tttgcactgg taacatactc atcctggttt tttatcaccg
    17101 ccagtttctt gatggtagct tcatcatgct ttaagaatat acgggccatc cggttgactg
    17161 tatacgaacg ataccccatc atttttaata catctgtccc cactcttaat gaggtatcca
    17221 gtgtttccct atagacatac atcattccgg cattcattaa ctggtacgca tcaaacctgt
    17281 tttctgctct taccaatatc cggagattag gaaaatgttt ttttattgta tcaatcatct
    17341 ctagtctttt ttccaggtta tctagtgcca gcacaatgat ctttgcctgg tctgcaccag
    17401 ctgcccgcaa caggtcatgc cttgaggcat cgccataata aactttaaag cccattttac
    17461 gaagtaagtc aaccctgtca gaatcataat caagaaatgt agcctgtaca ccatttgccc
    17521 gtaaaaaacg gccgattgta tttccaaagt gaccaaagcc ggcaatgatg accggatttt
    17581 tttcatcaat cttatcatct tcccgttcat tctctttttc tttagtaccg aaccggggct
    17641 ggataatttt ttcattaact aaaatcacaa gaggggttac agccatactg atcgctacta
    17701 ctgctaccat gatgtcaatt gtatctttag gtaatacacc ttcctgcagt gagaatgaca
    17761 aaagaacaaa gccaaattct cccatctgac ttaaagcaaa accaaagatc aggttttggt
    17821 caaaccttaa tttgaaaagc tttccaagaa tcaaaaggac tattcctttt atggtcatga
    17881 tcaggaagac aagacccagt attaaaacgg gtttttgcat aaccagctga aaatcgatcg
    17941 atgcaccgac agcaataaag aacagtccca gcaataatcc tttgaaaggc tcaatatcac
    18001 tttcaagctc atgccggaac tcgctgttgg ctaatacaac accggccagg aatgttccaa
    18061 gggcaggact aagacctact tgcgtcatta ataatgtgat agcaataact aaaagcaggg
    18121 ctgaggctgt aaatatttcc cgtaatcgtg tgcctgcaac aattcttaat aatgggttga
    18181 tcaggtaccg gccagaaaat attattgcaa atactgcagc cagtacaatc agcgtttgca
    18241 tccatcccga taagcttgcc atccaggtat tttttcccaa cgcccctcca ccggtcagag
    18301 gctgtaaagt ggccaataaa gggaaaaagg ccagcatagg aatgaccgca atatcctgga
    18361 aaagtaaaac ggaaaaagag tgacggcctg cagaagtttt catcaggcct ttttcattca
    18421 gtgtttgtaa tactattgct gttgatgaaa gggcaatgat cattccaagc gctaaaccct
    18481 gctgccaggg aatgtggaat gattgagata tagcagtgat aataattgtt gtgatcagta
    18541 cctgtaatcc tccaagtcca agaattgatt tccgtagctt ccataataat tcaggctcca
    18601 gctccagtcc gatcaaaaat agcatcatta ctaccccgaa ctcagcgaaa tgcatcagat
    18661 catggccttc tgaaccaatc aggttcagta atgccggacc aataattatt ccagccaata
    18721 aatatcccag cactgaacca agcccgatac gcttagctaa gggcaccatt actactgctg
    18781 ccgacaaata aacaaaagcc tgaaaaaata aagaattttt atccatgaat tatgatttga
    18841 cctgctcagg aacaggtaaa aggtcattta aataatttac agactggtat tcatcttcgc
    18901 tgatacggtt gtgcaccaga gatatcaata attgcaggta ttgtaccgca tgaagttcaa
    18961 tatctgcctc tgacatttta tgtgtaccat gtactacaaa gggaggcatg tagtgcatat
    19021 tacagagggt tgcggtttgt tcaaatgggg taaggaattg ccggatgctg aacctgttcc
    19081 tgccggtttg gctataggct tccagagacc cgccgcagga taaagcatta aaaatacgtt
    19141 tacccgtgag catcttgcca tttgcaccat atgcccagtt atgttccagt acaagatcct
    19201 gccattgttt gatcatagct ggggcactgt accagtaaaa agggtgttgc catataatga
    19261 tatcatgttc cagcaataat tgctgttctc ggtttatatc aatatccagg tcaggatatg
    19321 tttcatataa atcatgaaaa taaacatgct ccagatggcg ggtatgattc accatccgct
    19381 tatgaatcct tgacttttcg aagacaggat gtgcaaatag aataagcagt ttcgccatac
    19441 ataaaaataa ggcaaaatgc tcaattgtaa tatagaaaat cagtacagta ttctgcaaag
    19501 aaactacaga tagatactta taaatatcag gtaaataagt tcagcgatta tatttatttt
    19561 tttgaatatg ggcctgcatt tttaatgatt ttggatttga ttttttgccc cctgtttaaa
    19621 tgtgcactag ctaaattaac agatcgtacc tgttcctttt tggttgatgt tattaatatt
    19681 ttaaccagta atgcttttgg ggcacaaaaa aaccggcaat attcctgaat aattcaggag
    19741 cattgccggt tttatatact gcagtacgga ttaaagaata actacactat tggtgccgcc
    19801 ataaatattc ggctcctgtt tgtcagcttc cggatcattg atccattcag tttcattcac
    19861 caggtatttg aactcatgtt tagaatcttt ggggagattc acatctgcac tgaaagtgcc
    19921 atctttcttc ttgctcatga ttatcgaact ttcccagtcg ctgttcagtc ccagtatttc
    19981 aattgtttct gcgttctcca ctttaaagga aaattttact ttacagtagt cttttgtctt
    20041 gtaataggtt ttttgtacca tttgccggtt tttgggttaa aaataaaatc tataagtaat
    20101 tcattgacaa acagcaatcg gcataattgt tacgttcagg taaccgcttt taatacttga
    20161 aaaagtaaaa ggtaacccgt tcaccgggaa ttgataaata acaggttatc cactttttaa
    20221 gcccacaatt caatcatatt ggagtttgac aacactttaa ggagcttgac aacactttaa
    20281 atagacaatt tttaaacgtt ttcaccgttg taaatgggat ccaatctatg taaaaccaat
    20341 atctgtttgt atgaaccacc cgtactgcaa tgaccaggca tatattgccg aatcattttt
    20401 aattgatgag gattatatcc tgcaaacctc aagattagct acttttaaaa ttaactctta
    20461 caagctttca ctgatcaaag ctgctttctg taaaacacgg gaagagattt acaataacct
    20521 ccagagtagc ttattcaatt ataaagaccc tactatttta ttagcagacc tcggcttttt
    20581 tgaggaagaa atagataatt gaaaatcccg gatttttatt ggtttggtaa ttttttctta
    20641 cctgtattta ttaaattaat aatgattggt ctgttcaatc agtgaatgtt ccttttaaag
    20701 gatttaataa tttttaactc attttgtacg cttaagtttt tataattcca gtcaggagaa
    20761 gagaaataaa agtcttaatt tccttctttc taattgtgta tcatgaaagt atcactccgt
    20821 ctgcgaagaa ctaccattgt tttattcatc attatttttt tggggtttat tcttgcacaa
    20881 tgtgtcaaca ggcagggcga aaaaaaaacg tcaggcaacg acaatgctac tgctgaaact
    20941 acttcttcgg tcagttattc gctattttcc ggatctgcga aatgtgcggg atgtcataag
    21001 gatatttatg acaaacatat tcaaacggca cattatctta cttcacaacc agccgaagaa
    21061 aaatatatca gcggaagctt taagccgggt aataatacct attcttataa tcccctatta
    21121 cggattgata tggaaaaaag ggatagcggt ttttaccagg tagtatatta taaaggagaa
    21181 aagaaaaaag agcttcgttt tgatattgtc acagggtccg gtgtgatggg gcaatcttac
    21241 ttgtactggc ggaataataa gttgttccag atgcctgttt cctacttcac agctgcacaa
    21301 cgctggtcta acagcccggg atttcctgat aaagtaatgc tggacaggcc aattactgcc
    21361 cgttgcctgg agtgtcatgt cacctatgca ggaattagct ctgcaccggg tgtggaaccg
    21421 gaagagtatc atcatgacca gattatattt ggtgttgact gtgaaaaatg tcatggccca
    21481 gctgcagacc atgtagcgtt tcatactgag aataccaggg atacaatggc aaaatatata
    21541 atcaattctt cttccctttc acgccagcaa aacctggata tgtgtgtact atgccatgga
    21601 ggtaatattc agaaaacaaa accttctttt acctatactc ccgggagatc attggctgat
    21661 tattttaaaa tcgatacact cagcatggta gctgtgcaaa atgaaaatat tgatgtacat
    21721 ggaaatcagt ttggtttgct acggtctagt aaatgttttc aacaaagcac tacgatgaca
    21781 tgcaatacct gccacaaccc gcatgagaag gagaggggta aaacggctat attttctcaa
    21841 cgctgtatga gctgccatac tccgggccat gataattttt gtaaattaaa attatcgctc
    21901 actcaattga gtaaaaattg tattgattgc catatgccgg caaggaaatc catggcagtg
    21961 gcggtttctc ttcccggaga agaagtgccc cgtgcagcat ttgttcgttc tcactttatc
    22021 agtatctatc cagacgagac caaaaagatg attgagaata ttaataaatg aaggactaca
    22081 cattttaata acactttcat ccaaagtagc tgtaaagtct gcactgcata ccagataaag
    22141 cgttttaaca atacataatc acccgttaag tctatattac aggatcgtta tacaacaata
    22201 atgatttctt tgaatgctta ttatcttact cttttttgta gctcattggt acctttcact
    22261 tttcatgcaa acttttcttc aacaccgttt tgccgcccat gcagctttta ctatgaacaa
    22321 attctgggaa cgttttttct acggacttgc ctatatttca caaggttcat cttatgtgag
    22381 tccaagagtg tatgcaatta tgcaccgcat gcatcatgct tatacagata cagataaaga
    22441 cccgcattca ccaacatttg ataaaaatct ttttgtgatg atgtggagag tgggccggtt
    22501 atctaacgga atatataatc agaaagtaga attggaaccc aggtttctta aaaacctgcc
    22561 tgactggcct gctttggata aaatcggcca ttctgtttgg tcaagactag gatggattgc
    22621 tgtttatgtt atgttttatg tacagtttgc cccatctgca tggtggtatc tattaatacc
    22681 atttcatatt atgatggtgc cttttcatgg catgatcatc aattggtttg ctcacaaata
    22741 tggttccgtt aattttaact tgaaaaacac atccaccaat ttattaaagg ttgattggct
    22801 gatgatggga gaagggtatc ataataacca tcataaaaat ccatccagta ccaattttgg
    22861 ctttcgctgg tatgagctgg atcctactta tgtcattatc cgtattttta gcaggctaaa
    22921 gatcatcaaa attcataaac cggttcaggc gttgtaacat taccagcgcc gggcatattg
    22981 taaaaaaaaa gagccctgtt tggggctctt cattatggga gaatttattt ctttaccatc
    23041 ttttgttatc tgatcttgct ggcttttctt ttgcaacaga aacagacata gaccttccat
    23101 caatgtcttt accattaagc cctttaatag cctggttggc ctcctcatca gatcccattt
    23161 ccacaaaacc gaaaccacgg ctacggcctg tttccctgtc attgataact ttcgctgaag
    23221 aaacagcgcc aaattgttca aataattttt tcaggtcatc atcaccggtg tgaaaactta
    23281 agttcgaaac gtacatgttc ataaaacaat aatttaaaat aaaacaataa aatacctgtg
    23341 acaaaggctt gcagcaaaaa gtaaagagga gattaactgt tactatgacg atgagtaaaa
    23401 attgatacaa atccggactg aatgttgtaa gagcttacaa tcacctggtg aagatatggt
    23461 attataatgg caatctgctg attattattc caattccaat ccctgctgac tttaagccag
    23521 gtagaaggtt ttatcacaaa gtttgctcta ttgcaattcc ttaaaatggt tttggaacag
    23581 aaaatgagaa tgtactgcct tcacccggtt ggctttcgat atatagttgc ccgccatttt
    23641 tgaccaggaa ctctttacaa agcattaatc ctaaacctgt accagattca ctggcggtac
    23701 cttttgtcgt gaagtaatca ttattcctga tttttgccag tgcctcgtgg ctcattccca
    23761 tgccgctgtc tttaacatat atttcaataa aagaggggtg ttcgtgcaca ccaatcgaaa
    23821 ttgttccttt ttcaggggta aattttatgg cattggataa aaggttacgc aggaccaggt
    23881 tcatcatgtc tttatcaata aaaccatgta cacggggggg ggcatcattc agcatgatta
    23941 ttcctttgtt ttttgcctgt aagtgcaatg actggtagac ttcatctatt gacttttcaa
    24001 tgtctacaac ttcgggatta actgttgttg actgcatttg tgtcttagcc cattgcaaca
    24061 ggttttccat cagccccact gcataattca tatctttcag tacttccgga atattattct
    24121 ttaattcttc tgctgacata ttcttttcat ttgcttctct gaaaagattg cgtatagcat
    24181 acatgggggc tttcaggtcg tgagatataa tgctgaatag ttttgttttt actgtattca
    24241 gttctgccag ctgagtggcc tgttgcttaa gcaattcagc actttcttcc agtttattgg
    24301 tttgtttttg tatctgttca tttttgccct ctaacatgta atttttactc agtatccttg
    24361 tgtggtaaat atcattttct tttttgatga ggaacaatcc ataaaaaata aataccagtg
    24421 caagcccctg gttgaacagg tagagaccgt tgttgaattc ttgtaactcg taaggttgat
    24481 gttctataac gactgccagt aaaaaataac tgaccattga aaagcagagg caaaatataa
    24541 tgtaacctgt atcttttaaa aagaaaacgg agaggatgcc aaaaagtata aaagtaagac
    24601 cgactccggg attaaaccca tagaggtaaa ccacacaggt gaaaaaagga tacaggataa
    24661 aataaaccag caatgcagtt tcatgtttac gctgataatt gaagaataat acgatgaagc
    24721 tgaccagggc cggaaggcat gctataatcc aaatactaac cggtaactga ctgttactga
    24781 acatcccgcc aaccggaatg agtataccgg taattaattg aaagaaattt agtttattga
    24841 aaatgcccag ttttcgctga tcatactcct ccatggtttc tgtaaaacca atagccttga
    24901 caaatcgcaa taagacctgg tattgttttt tgagacgaag aaaatatccg ttaagtttta
    24961 cagcagatag cttagaacgg ttttgggata ttggttgcgc agtaatttcc attggttttt
    25021 cctttaggct tggataagac aaagtaaaag tattaagcgg gaatgtacta agaaagggag
    25081 gtaagattgt cgtttaatcg taaaactacg agccggatat tgcggaaaac tgaaatggga
    25141 aacctgagta taaatagcca ggctatttaa aagtgttctg aaagaatttg tcttcaattt
    25201 caaatacgta taccgggtag ctcgattgtt taattgtgaa atcccaatat tattcagtaa
    25261 aacaataagt acagaaaaat attttatccc ggattatata ttttctgttt aggcagttta
    25321 aatgatttta aaaacttctc agcattagaa aaaatcgggc taaacaaaac tgattttttt
    25381 ttattttcat gaaatttgat aatacaaatg aaaatacaac cggcgatacg atataaagtc
    25441 aggcaggtaa ttaatattac cgttttatgg cttatcatcg gcgtgttggt tgaaatacat
    25501 aatgctatta attataaccc ggaaaagggt agttttttta tttattttat tttcggaggg
    25561 aatgctttac aacatcttct cattacttcg ttaggtcctg ttgcaggagg actgttagca
    25621 ggttctttta ttgtttttta tcagcgggaa aaaatgaaag gtaaaaccta tgggcagaag
    25681 ttgtttattc attcattact ctatctgatc tttgtcagtc tttgtatact tatagttggt
    25741 actattgggg cgatcaatga ttcagcagcg gcttcattct gggaaaaatt tgatgcggct
    25801 gtttttaatc tcagggtttt gcggttgctg attacctggt attttattgt actcttcaca
    25861 attttttttc ttgatatcag tgaaaagtat ggaccgggta ccctgaaaaa aatgctcctt
    25921 ggcaggtatc attcaccggt acaggaagac agaattttca tgtttctaga tttaaaatcc
    25981 tctactacta ttgctgaaaa gatcggcgac gagttttatt tccgtatgct taaatatttt
    26041 ttccagctgg ccacggaacc cgttttaaac tgtaaaggcg aaatatatca atatgttggt
    26101 gatgagataa ttatttcctg gaaccgaaaa gagggtttgc aaaatgccaa ctgcctgcaa
    26161 tgcttttttt caatacagga aactatccgg gctaaaagcg attatttcaa aactcagttt
    26221 ggaacagtac cggattttaa agctgctatt cattcaggaa ttgttactac gggggaagtc
    26281 ggggtcatta agaaagatat tgtctatagc ggggatatcc tgaatactac ggccaggatt
    26341 atgtcccttt gtaatcaata taatacttcg ttgatcatat ccggagttat ttacgaggaa
    26401 ttaaaaagga ccgccgggta tgagtttaac tggctggata gtcccggttt acggggaaaa
    26461 acaacaaaaa cgccggtctt tagtgtaaac aggaaggtat aagaaaagaa atttctccgg
    26521 attttttttt ggaaaaaaat taataaaact gccagaaaaa caggttttat taacttcgtt
    26581 cctgacatta tccaatggct gaacattcac atcaactcgc agcgattctt tttgccgaca
    26641 ttgtcggtta tacggctatg atgcaggaaa acgaagaagt agctgtggaa aagatcaatc
    26701 ggttccgcga agtagtggaa ttgatcgctg aagaactgaa tggcaagatc atccagtatt
    26761 atggtgacgg ttccttatta ttgtttcata gctcaactga tgcagcggaa tttgccaaat
    26821 tattgcagga agatctgcaa aacgaacccc gtatacctgt ccgtattggc attcatatgg
    26881 gtgatgtgat ggtacacagt ggtaatattt ttggggatgt tgtcaatatc gcatcccgta
    26941 ttcaacagct ggcaccggta ggtggtattt atatatcgga gatggtgtac cgcaatattg
    27001 caaataagaa ggaactggaa tccgttttta tcagggagga aaaattaaag aatgtaaaaa
    27061 ttcccatacg catatatgaa atagtcaccg ctggcagtca gcccttcaca caacaagctc
    27121 ctgcagctga gccggtttta ccttctacca atgctaacag tattgcagtg ttgccatttt
    27181 ccaaccttag cagtgatccg cagcaggatt attttggaga tggccttact gaagatatta
    27241 tcacacagct ttccaaaata agggcttaca aagtgatctc ccgtacttct gtaatgcaat
    27301 acaaaaaatc gccaaagtcc ataattgaga ttggaaagga tctgggtgta gcactgatat
    27361 tagaagggag tgtacaacgg catggggaac aggttcgcat taccgcacag cttattaatg
    27421 caataaccga tgaacatatc tgggctgaat catatgaccg gtcaatgaat gatatttttt
    27481 ctattcaacg agaagtggct ttagccattg ctgctgtttt gaatactaaa ctttccaata
    27541 gagaaacaag agaacttgat tacattccaa catctgatct gcaggcttat gatctttacc
    27601 tgcggggtaa gttattaatg gaaaacagga acaaggctga cctattgacg gccagagacc
    27661 tctttaccca ggctgtgaac agggataaga cttttgcgat tgcttattcc ggtttggctg
    27721 atacgtacct gctttcatct ttccgtggat atgaagatcc ggttatgatg ttgtggcagg
    27781 ccaaaaaata tattgatact gcagccggac ttgattcatt ttccggtgaa acacaggctt
    27841 cattgggata ttggtatttt cagagttttg actggcatgc ggccgaatta acttaccgga
    27901 gatctatcag cctcaatgct aatcaatcta atgtatacct ctggctggca attttgctgc
    27961 aggctaaggg agagaatgaa gaggcgttga aagtatatga tttaggaaca gaggtcaacc
    28021 cgatgtggga tttcttactg aaaaataaga tcatcgcatt ggtgaatatg aataataaag
    28081 aagcggcact ggaactgcag cacaccctta ttgaaagagc ctcacatgat agtcacctga
    28141 aaagaaaaag atatgaagag ctttccaggt tgtactggtc tttcaatgat aaggaagaag
    28201 ccattactgc tgccaaaaaa tcgggggatc ttgccttgat taaattctat tcggatggag
    28261 accattccct gctggagaaa agacaagatg atcattattc tgctctccgc aaccgtagtg
    28321 aatacatttc tcagttctgg atgggcctcg actatgccca tgccggtgcc agggaaaaag
    28381 cattggaatg ttttaataat gctattgctt tgaaagaggc cggtatttca ttgctgctca
    28441 tccgtgattt tgaatttttg aatatcaaat acctgagtat ggcccttatt acccggaagg
    28501 ttaagcaatt gattaatttt taatgaaaag ctgaatggca cactgttgct atattattct
    28561 tgtta
//
LOCUS       DAORTI010000034        28386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1219_length_28386_cov_6.214147, whole genome shotgun sequence.
ACCESSION   DAORTI010000034 DAORTI010000000
VERSION     DAORTI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28386
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_28386_cov_6.214147"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PLO70_06415"
     CDS             complement(<1..1245)
                     /locus_tag="PLO70_06415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQZ74129.1"
                     /translation="METYGKILLIAMPAFLLLVLFEKWYGWRKGFDTVRNMDMISSLS
                     SGVTNVTKDVIGLYLVILSYPFFLEHLALTQISNTIIVYIIAFFALDFAGYWVHRLQH
                     VANFFWNGHIVHHSSEEFNLACALRQSISSIVKTFAIFLLPAALLGVPAAVISVVAPL
                     HLFAQFWYHTRHINRMGFLEKIIVTPSHHRVHHAINKEYIDKNYGQIFIFWDKWFGSY
                     QEELPNVPPVYGITRPVQTWNPIKINFQHVWLLIKDAWRTKKWKDKFTLWFKHTGYRP
                     ADVTTKYPVYKIEDVYHFNKYDTKTSPAFSVWTWFQLLMILGFVSYLFGNIALIKELG
                     PYNIFWYGGFIFLSVYALTDLMDRNASAIIIEIVRSGLGLYFIYTQDDWFGASTYLAA
                     LKYILGAYFIFSIVVTGWFVIKH"
     gene            1412..2440
                     /locus_tag="PLO70_06420"
     CDS             1412..2440
                     /locus_tag="PLO70_06420"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HQZ74130.1"
                     /translation="MKHIFECIDVHTCGNPVRLVKEGGPVLHGNNMSEKRQHFLREYD
                     WIRTGLMFEPRGHDMMSGSILYPPHDPANDVAVLFIETSGCLPMCGHGTIGAITIAIE
                     EGLIKPKKPGVVRMEAPAGLVLIDYKEAGSQKPEIGRLSKVESVKLTNVPAFLHSTEL
                     LAGCPELGELVIDVAYGGNFYAIVDVQKNFKGLEYYTADKLIAWARELRKNINAKYEF
                     VHPEDATINGCSHILWTGAVLDKTSTARNAVFYGEKAIDRSPCGTGTSARMAQWYTKG
                     KLKMGDEFIHESIIGSKFTGRIEEELVVDGKPAIRPSVEGWAKIFGYNTIWIDKEDDP
                     YAYGFQVI"
     gene            2481..3731
                     /locus_tag="PLO70_06425"
     CDS             2481..3731
                     /locus_tag="PLO70_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ74131.1"
                     /translation="MKAIIIGGGIIGLSSAFYLQRSGWEVTVIDKGDFLDNCSYGNAG
                     YISPSHFIPLATPGIVKKGLKWMWNSKSPFYVQPRLDWNLINWGLKFMQSANTKQVEE
                     AAVPLRDISLLSQQEYEGWTKIPGFDFAYEHKGLLEIFQTQEGADHAQHILEKAHQLG
                     LSDTVILSKDELSKLEPQTTINAIGALWFKCDAHCYPDKLMGSLISYLRSAGVQFIAN
                     NEVTGFGTGGGKIKKVLTTTGSFEADEVIIATGSWGRETARLLGIKIPLMPGRGYSVT
                     LEDSPYKINFPAVLVEGRVALTPMDGNKIRFGGTMEITSHQTPPRMNRVQGILDAVKR
                     YYPDFDVALPPKEKIWYGYRPCSADGLPYIGRVKKFANLAIATGHSMLGLSLGAGTGK
                     LVSEIVNNRPTSMDIKPFSVERFS"
     gene            3797..5398
                     /gene="bshC"
                     /locus_tag="PLO70_06430"
     CDS             3797..5398
                     /gene="bshC"
                     /locus_tag="PLO70_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HQZ74132.1"
                     /translation="MNCTSTRLPYRQTGAFSKLAMDYIDQAEALKPFFAHPPTVKGLQ
                     QAIEARKQFSTNREILVQELKKQYGGVETGDKVKNNIDSLLSADTFTITTAHQNNIFT
                     GPLYFIYKIVHAIKLADTLKVSLPQYNFVPVFYIGSEDADLDELNHIHLGGEKLVWVT
                     KQTGAVGRMRVDKELLKLITIMEGQLLVLPYGHEIVSLLKQFYKEGDSIQQATFRFVN
                     ALFADYGLVVLLPDNPSLKKLMVPVFTDELLNQVASDIIEVSADKLEKGGYKVQASAR
                     EINLFYLKDDLRNRIELKNEKYRVVDSSLVFSEDEMVKELQQHPERFSPNVILRGLYE
                     ETILPNIAFIGGGGETAYWLQLKGLFEHYKVPYPVLILRNSFLVLEQKWKEAVSKLNF
                     TMEDFFLPEQELLNRLVARDSKNQTKLNGSMTKLEELYESFKKQASTVDASLEKHVES
                     LKSKTVYRLQELEKKMLRAEKRKFSDQQRQIHTIKSHLFPGNGLQERIDNISYYYAKW
                     GTDFIRSLYDHSLTLEQEFVVLQEK"
     gene            complement(5406..7568)
                     /locus_tag="PLO70_06435"
     CDS             complement(5406..7568)
                     /locus_tag="PLO70_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ74133.1"
                     /translation="MRKFLGLAFLLLFSAFLSGVAGQTPLKARDTTIKGPQTFAIVMG
                     ISKYKYIRPLTYADKDAELFRDYLRSPGGGNVKEENIFCLLNEQAINSVFWTKGFQWL
                     KAKQLQKGDKLFIYLAGHGDAIDEDQFFFLSYDCNPAGDKNNYLVGGAIQLYNLKKKI
                     ANETTKGVEVFFIMDACRSNELPGGTAGQSFLNTAITEKKVGEIMMLATAAGQESLED
                     ASIGNGHGLFTYYLVDGLSGVADSIGVPDEKVTFQEIKRYVERNVPSVAMQRFKKKQD
                     PYFCCDENSEKIISTVNTAYLEKWLQTKKMQQRGPGNSFHGTLVKPRRNINDADTALS
                     ETYNKFYQAIKDNNITGGSSAEFYYQQLDRKYPGNPYTLDAKSTLAVEYINYAQAIVN
                     RYLDCNTATNKEKQQNNEAGTRLEKAISILREDDADFANSLLGRMFFLKASGTYPDAA
                     TAFQYAYAALAINPDAAYIHNRLATLHLENNRRDSALFYAEQASRIAPKWLCATATLA
                     LVKNLSSKKNTVKKSSVGFTFGGGINQSNPTFRDNSNTNVIGIDAKGAGILNIGVIYN
                     INIGKNLAIRPATTLTFGNTELVYLRRNIVGGEIIRELVPLKNTAINIALPMIIRFNN
                     KQAAPYFSLGPSLQYILGQDNTSAELLPVKKTLFMGDAGFGIDFTIPRTGMIISPELK
                     YTAGFTDMKDPAVSTVYSTNISSLKKQAFLLNICLRKK"
     gene            complement(7641..8561)
                     /locus_tag="PLO70_06440"
     CDS             complement(7641..8561)
                     /locus_tag="PLO70_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74134.1"
                     /translation="MTRLLLLLSFFFISCSKKHKLQETIQLPHYPSSSGISYFKKQIY
                     ITGDDAKHLLILDSNYLIKDSVTLYSHTEYKIPKATKPDLECVTITATNKLLILGSGS
                     SIPYRNTGWLIDPVTKQKDSLRLDTFYRRLKNNGLEEINIEGVCAIPGNIILSNRGNK
                     TNRKNFLVLTRKDFWINQTDAPVTLIRVGANTDSTVFNGVSGLDYSNKSDRLLLTVST
                     EDTRNSIDDGTIGKSYLWIVENISAKKRWKAINPDKIIDLEDVDDRFKGQKVESVCIL
                     AESDNSITLLLAADNDNGSSTLFRVIISKE"
     gene            complement(8609..9154)
                     /gene="purE"
                     /locus_tag="PLO70_06445"
     CDS             complement(8609..9154)
                     /gene="purE"
                     /locus_tag="PLO70_06445"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQZ74135.1"
                     /translation="MSEPQTTNHQLQTVLVGIIMGSDSDLNVMQAASDILKQFDIPHE
                     VTVVSAHRTPHRMIEYAQTAKERGLKVIIAGAGGAAHLPGMVAAVTTLPVIGVPIKSS
                     NSIDGWDSVLSILQMPNGVPVGTVALNAAKNAGILAAEILGTADETVSVKIAAYKNTM
                     NNEVIEKAEKMKREGWENKFD"
     gene            complement(9147..9488)
                     /locus_tag="PLO70_06450"
     CDS             complement(9147..9488)
                     /locus_tag="PLO70_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74136.1"
                     /translation="MYPLTFKEPIASDFRLKDQMRGSCGSIMDNIAEGFDRGSKNEFT
                     NSLTIAKGETGELKSQLYRSLDNKYITEEIFRELYSLADELTRMLTGFINYLNKSKIK
                     GQKFKNRTKDE"
     gene            complement(9613..10923)
                     /locus_tag="PLO70_06455"
     CDS             complement(9613..10923)
                     /locus_tag="PLO70_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ74137.1"
                     /translation="MKRFLLPLLVLISFSPAAQRLLTLDEAIATALKNNYDIQLSKND
                     SLVATLNYSYRNAAFLPRLNGNINGTFNNNDQRQEFNDGTKRELDDIKSNNYNLGLQL
                     NWTLFDGLKMFATRDKFAEFVKLGELGIKNQVVNTIAAVITNYYSVVRQKQQLKAIEE
                     QMSVNQERVKIAQYKLDIGVGAKPDVLQSKVDLNAQKAAQLTQQTLIAQLREQLNQSM
                     NVTLNTNYDVSDTIPLDMNLVLVDIRDIATKNNPLLLIAEKNIDIANYTLKERIADKY
                     PVLSLNAGYNYQRNNNKATVNPFQPLYSRNIGRNIGFNATIPIFNNFTVKRNIQQAKL
                     DIQYQRLVYENQKSVLDLGVINAYKDYEQQKKALNLEEENILLAKENVSIALEVYRLG
                     ASTILQLRDAQQSLENAYNRLIAARFNAKLAETELLRLKGDLVK"
     gene            complement(10940..14065)
                     /locus_tag="PLO70_06460"
     CDS             complement(10940..14065)
                     /locus_tag="PLO70_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQZ74138.1"
                     /translation="MNISELSLRRPVLAIVMNIIIVLFGVIGFKFLGVRDYPAIDPPN
                     ISVRTSYPGANADIIETQITEPLEKAVNGIAGIKNITSSSSNGSSNINVEFELGIDLE
                     TAANDVRDKVSQAQRSLPADLDAPPVVSKADASSDAILSMTVQSNTRNQLQITEFANN
                     VLVERLQTIPGVSGIQIWGEKRYAMRIWIDPAKLNAYSIAANDVQAALLRENVELPSG
                     KISGSATELTVRTFGRLNTEEEFNNLIVKNAEGADIRVSDIGEAVLGPENEETVLKGN
                     GIPMIALAVIPQPGSNYVSISDEFFKRLEQIKKDVPEDIKINIALDQTKFIKKSILEV
                     QETLMIAFLLVVMIIYAFFRDWIIAIRPLIDIPVSLIGAFFIMYLMGFTINVLSLLAI
                     VLATGLVVDDGIVVTENIYKKIERGMNKYQAALEGSKEIYFAVIATSITLAVVFLPII
                     FLQGFVGRLFREFGIVVAGAVLISAFVSLTLTPVLNVKLSRKNPSKHPWLYTKTEPFF
                     RWMENTYQAALRKFMAIRWMAFILIALCLAAIFFVGRNLQSELAPMEDRSQFRLQLSA
                     PEGTSYDAMDKYVNRLTNLMLDSVPEKDMVLSVTSPGFSGSGNANTGFVRITLIPPNE
                     RERSQNEIVKMVNKNLPKYNEGRAFAIEEQTISVNRRGGQPVAFVIQNNNFDKLTQVL
                     PKILEEARLSKVLLNADVDLKFNKPELRVEIDRLKAAQLGVSVNDISQTLQLAYSNRR
                     LGYFTKDGKQYQVMGQVARNDRDDPNDLKTLFARNNRGELISLDNLVTVSESNTPPTI
                     YHFNRYKSATISASLQPGFTIGDGIEEMEKIANKVLDETFATSLSGSSRDFKESSSNT
                     SFAFILALVLIFLILAAQFESFIDPLIIMFTVPLAIAGAVLTLSIFGHTLNIFSQIGM
                     IMLIGLVTKNGILIVEFANQNRLKGMKRTEAAIFSATQRFRPILMTSLAMSLGALPLA
                     LSLGAAATSRIPLGIVIVGGIMFSLVLTLFVIPAMYSFLSSAHKKNEVEQFIHPATQP
                     VQPIQPVVEQV"
     gene            complement(14128..15189)
                     /locus_tag="PLO70_06465"
     CDS             complement(14128..15189)
                     /locus_tag="PLO70_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ74139.1"
                     /translation="MRIITIFLLAGFILTLSACGGNKKKVEAPQHGGGAKTPPMRVDG
                     YIVRAESFGESIEVPGNIVANEVAEIHPEVSGRIVYLNVAEGRYIGKGTLLAKLYDGD
                     LQAQLKKLEVQLSLAQKTEERQAQLLKIQGISQQDYDISLLQVNNLRADIGIIRTSIA
                     KTEVRAPFSGMLGLKNISPGAYVSPASVIAVINQTDQLKLDFSVPEKYTGQIKVGQLV
                     TFTFEGSDKKLGAKIIATESNVTENTRSLTARALVTGKDKALLPGTFAKVHLSFAPDT
                     NAILIPTQAILPQARGKKVIIYKSGNAMFTDVTTGVRDSSRVQITDGLKRGDTIVVTG
                     LLSVRPEAPIQIGKIINSQ"
     gene            complement(15329..16450)
                     /locus_tag="PLO70_06470"
     CDS             complement(15329..16450)
                     /locus_tag="PLO70_06470"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQZ74140.1"
                     /translation="MLKVGILGGGQLGRMLLQTAANYPVETFVLENDKECPAAHLCHH
                     FTKGDIKEFDSVYNFGKNLDAITIEIENVNVEALEKLETEGVKVFPKPSVLRTIKNKI
                     LQKEYYRLHQIPTADFIITNSLSELKQAEDFLPAVHKVGEGGYDGKGVQLLKAKEHIA
                     RGFDTPSILEKMVAIHKEIAQMVAINEKGETALYPPVEMLFDPELNLLDYQLCPAELS
                     TQTLYRVEAIALSVVRNFKSPGVFAVEMFVDKNGDVLVNETAPRVHNSGHHTIEAHFC
                     SQFDMLWRIILGYPLGNTDAILPSIMVNIIGAEGYSGKVKYVGLEEVLKIENAFVHLY
                     GKKETRPGRKMGHVTILSNEKQDLLHQSNKVKRTLLAKA"
     gene            16700..17830
                     /gene="hppD"
                     /locus_tag="PLO70_06475"
     CDS             16700..17830
                     /gene="hppD"
                     /locus_tag="PLO70_06475"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HQZ74141.1"
                     /translation="MNTKTITDKAAIQTDFLPLEGTDYVEFYVGNAKQAAHYYMSAFG
                     FQALAYAGPETGVKDRASYAVRQHKLTFVLTTALRTDNPIADHVYRHGDGVKALSLRV
                     PDATSAWEETTKRGAKSYMEPTRLKDNDGEVVMSGIHTYGDTIHLFIERKNYNGAFMP
                     GYRPWNNPYFKPADTGLLYVDHCVGNVGWNQMNPWVKFYEEVMGFKNILSFDDEDIST
                     EYSALMSKVMSSGNGFVKFPINEPAEGKKKSQVEEYLEFYNGEGCQHVAMATNDIVKT
                     VRDLQSRGVEFLKVPTSYYDDLLDRVGHIDEDLAPLRELGILVDRDNEGYLLQLFSKP
                     VEDRPTLFFEIIQRKGAKSFGKGNFKALFEAIEREQEARGNL"
     gene            18013..19224
                     /locus_tag="PLO70_06480"
     CDS             18013..19224
                     /locus_tag="PLO70_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQZ74142.1"
                     /translation="MKNLFLMLISVNIVLVSYGQPSPTSSKGGNNSYSFIDYQKTFPR
                     PGEALERKEDTLQKQFEAKKLQWPAKYMYIRSFKYDSQLEVWVKNDWKEQFQLFKTYK
                     VCALAGSLGPKRMQGDYQVPEGFYYINEFNPRSNYYLSLGINYPNASDKILSDSLKPG
                     SEIYIHGSCVTVGCIPITDQQIDELYILAAHAKDQGQDFIPVHIFPIRYNVERSVKYL
                     ESLTKDDQDLKKFTDKLEDAFDYFEKYKQLPVVMINDKGEYVINEAAPKKLKGISTEI
                     KQQVKRPPAQHRERNIGLLAEAVHQWPQYPGGGEAFMKYLQVVGNEMTAFLPKGIKKA
                     FIQVEFIVDKDGVPVNLKVLKGFKEGEEFNDELISRLEQMGTWQPALLHEKPVAKKMI
                     QTITVEAPLAP"
     gene            complement(19236..20498)
                     /locus_tag="PLO70_06485"
     CDS             complement(19236..20498)
                     /locus_tag="PLO70_06485"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQZ74143.1"
                     /translation="MKVFKFGGASVNTIERIQNLAPIMNHWANEKIVVIVSAMGKTTN
                     ALEKVVDAFFAGKKEEALLLFEQVKQQHLTTSKYLLVTNYLACEAQLKDFFTEVEWLL
                     HDKPVRDYDYYYDQVVCAGELFSTTIVSAYLNEVGANNKWLDIRDIFRTDDDFRDATI
                     DWEFTKQKVNEVVLPELKHYNIVLTQGFIGATDENESTTLGREGSDYTAAVFANMLNA
                     DSLTIWKDVESVMNADPKQFSDAISIPELNYNEVIEMAYYGAQVIHPKTIKPLQNKNI
                     PLYVKCFSNPSLPGTIIHNKPVHHLPPIIVLKEKQVMIEMNSRDFSFIGEQHVGHLYH
                     LFEKLHIKPNLTQNGAISFLSVLDNWPEKIEKLAFEASAFLDVQVKKGLSLLTIRHYN
                     KEIVETLTMGKLILLRQQTPETVQFLLG"
     gene            20621..21634
                     /gene="fbp"
                     /locus_tag="PLO70_06490"
     CDS             20621..21634
                     /gene="fbp"
                     /locus_tag="PLO70_06490"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQZ74144.1"
                     /translation="MSINRKISTLDEFTIQQLRDFPNATGELSRLLRDIGLAAKRVNV
                     EVNKAGLVDILGDAGTVNVQGEEVKKLDVYANDQFTGVLRHGISCAGIASEELDDFVA
                     FDDEISKKSKYVCLYDPLDGSANIDVNVSIGTIFCVYKRVSPIGTPVTLEDFLQPGNQ
                     QVAAGYMIYGSSTMLVYATRRGVNGFTLDPSIGEWTLSHADIKCPELGKIYSVNHGNF
                     FKYEEGVKKYITACQKKDKTNGGPYTQRYIGSMVADVHRNLIKGGIFTYPGTTDKPKG
                     KLRLLYECNPFAFIVEVAGGKATDGKKRILDIQPTELHQRTPFFVGSKLMMEELETYL
                     KPE"
     gene            21637..22398
                     /locus_tag="PLO70_06495"
     CDS             21637..22398
                     /locus_tag="PLO70_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ74145.1"
                     /translation="MAKTIISVKNLVKNYGNFQAVKGITFDVMEGEIFGLLGPNGAGK
                     STTLEIIETLRPKTSGEIIVDGLCLDKQPNDIKKIIGVQLQTSGYYPGLNLLQLIELF
                     CGLYNREVDPMELLEMVNLKDKAKSPVKELSGGQKQRFSVATTLINEPRIIFLDEPTT
                     GLDPQARRSLWELVRNIRARGTTVIITTHYMDEAEYLCDRVAIIDAGKIIALDSPDKL
                     VDNLVASGFERPKEMKMANLEDVFISLTGHSLREG"
     gene            22404..22844
                     /locus_tag="PLO70_06500"
     CDS             22404..22844
                     /locus_tag="PLO70_06500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQZ74146.1"
                     /translation="MSIQNKLREFMQGKIEAQKTAGAAFMSENKLKEGVTELPEGIQY
                     EVIKEGSGHQPLLKDTVKAHYKGSLLDGKEFDNSFKRGQPFSAPLTALIKGWQIALPL
                     MKEGSHWRLWIPSHLAYGDRGAGSDIPGGATLIFEVELIEIVKK"
     gene            22855..23394
                     /locus_tag="PLO70_06505"
     CDS             22855..23394
                     /locus_tag="PLO70_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HQZ74147.1"
                     /translation="MPVTAVDPRYPIGEYAPQPFSIPRKVEWLADIKFLPLQLENAVL
                     NLDEAQLHTAYRDGGWTVQQVIHHVADSHINAYCRFKLALTEDNPTVKPYEEKLWAEM
                     NDVQKLPVNISLTLLHALHARWYESLKYVSDNDWNNRTIYHPEQKKTIRLWTLLGMYA
                     WHGKHHVAHITSLLERKGW"
     gene            23457..24005
                     /locus_tag="PLO70_06510"
     CDS             23457..24005
                     /locus_tag="PLO70_06510"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQZ74148.1"
                     /translation="MKWKTLSSEYLFNDRWFKVRRDKCETPGGKIVDPYYVYEFSTWV
                     GALPVTEEGKIIMVRQYRHALGETCLEIPGGCVDDTDTNVEYAIARELLEETGYSFTS
                     FDYLGKISPNPSTNSNLLHMFLAKGGKKVGEQNLDENEEIEVVEMTLDELKQLLRENK
                     IVQAMHVSCILYALEKLGELHY"
     gene            complement(24002..25372)
                     /locus_tag="PLO70_06515"
     CDS             complement(24002..25372)
                     /locus_tag="PLO70_06515"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQZ74149.1"
                     /translation="MTFQQTIDYLFTRLPMFSRIGAAAFKKDLTNTIELCNHLGNPHH
                     QFKTVHIAGTNGKGSVSHMLAAVLQTAGYKTGLYTSPHLKDFRERIKINGEMIPEQAV
                     IDFTETINPLIEKIEPSFFEITVAMAFDYFAQQKVDIAIIETGLGGRLDSTNIITPEL
                     AVITNIGWDHMNMLGDSLEKIAFEKAGIIKPGVPVIIGEVLPETLPVFEQVAKEKKSA
                     IHIVSDTRQTADWNWEKNELIVEVAEKHKTDHKIYHLDLPGIYQTKNLLTVLETCYQL
                     QNKGWIIPAQTITDALKQTKKITGLHGRWEVIHHHPLIILDVAHNVDGIKQLVRQIEL
                     TEHHHLHIILGMVKDKEAEKVLELLPKDATYYFTKAQIPRALPEEELARKAASFGLKG
                     KPFMNVNYSLYSASHKAGKKDLIIVCGSIFLVGEVSLRSVKEIWGKENSSFVDFDFLE
                     LIRFFD"
     gene            complement(25452..26333)
                     /locus_tag="PLO70_06520"
     CDS             complement(25452..26333)
                     /locus_tag="PLO70_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74150.1"
                     /translation="MNNAYTMSASLEAQYRSRAAMITAGFAGLAILLMFLLKWKLPVF
                     EKIVELPGIEVELNLPPDPPVPFDEGGGGGGNPVQAAGNPGIAQAAPMPPGTTEDSKD
                     IEEDNNSTSPAINKPVATKPKATAITNTSITKTEPKEIKTPAPPRPKAVMDGKNITGT
                     QKGGNNADNYDQAGGKGTGYGVGNGSGVGGGNGNGIGGGNGSGTGVGNGPRVTRGDRK
                     IVRSYSFEGDLDKATIYANVSVSPDGIGKFVSIAKGSSNSSNAYKEAIIRYLQNIKFD
                     KADHESMITVQFNFRVN"
     gene            complement(26464..27366)
                     /locus_tag="PLO70_06525"
     CDS             complement(26464..27366)
                     /locus_tag="PLO70_06525"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQZ74151.1"
                     /translation="MNHSAAKNIYTGIGLAVLATFIWSWNFIVARGIYKEIPPVSLAF
                     FRWLTATILIFPFAFKYLKKEWPVIKQSWIYFLFAAATGIAMFNTFVYVGAQYTTAIN
                     LALIGTTSSPIMSVIFARIFLNEKISGTRVFGITICIAGILFLLSKGNFQNLLQLKFT
                     KGDSWVLLAGFAFAVYNTLVKKKPAAVSPVNFLFIVFSMGTLLLVPFYIQETAHAAPI
                     SWNVSALLIILYLGLGASVICFWIWNIAIRNLGAGRAALFGNLIPVFSTMEAMLILGE
                     KINWVHLVSFVLVILGLLIANLRK"
     gene            complement(27373..>28386)
                     /locus_tag="PLO70_06530"
     CDS             complement(27373..>28386)
                     /locus_tag="PLO70_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQZ74152.1"
                     /translation="APEAPILPLFCYTAAGWLDEKFYVPAIRIEPDIRQECAGYDAKR
                     VETGAQELLKNYPHNRLVAHLMNNCALTYSCPAARNFALGRWECPVPASPACNANCVG
                     CISLQPDEEQIVSTQDRLNFKPTPEEIVEFTVPHLESAPFPIISFGQGCEGEPLLMWE
                     TIRDAIIEIRKHTKKGSININTNGSNPEAVKILCEAGLDSIRVSTNSARREIYMPYYR
                     PNNYEFDDIIESLKVVNSYGGWTSINYFVFPGMTDSIAEYEALRKLIKETGLKMIQWR
                     NFNIDPDWYLGKIGVTDTGECMGVKQMQELIREEFPDLKFGYFNPPMERIKGKFDMDF
                     AHY"
BASE COUNT         8174 a         5605 c         6082 g         8525 t
ORIGIN      
        1 atgtttaatc acaaaccaac ccgtaactac tattgaaaaa ataaaataag cccctagaat
       61 atacttgagt gcagcaagat aagtgctggc cccaaaccag tcatcctggg tatagataaa
      121 atataaacca agaccgcttc ttactatttc aatgattatg gcagaagcat tccggtccat
      181 caggtcggtg agtgcatata cactgagaaa gataaaacca ccataccaga aaatattgta
      241 agggcccagt tctttgatca atgctatatt gccaaataga tagcttacaa aaccaaggat
      301 catcagtaat tgaaaccagg tccagacaga aaatgcaggt gaggtcttgg tatcgtattt
      361 gttaaaatgg tatacatctt caattttata caccgggtat ttcgtcgtca catcagccgg
      421 gcggtaaccc gtatgtttaa accaaagggt gaatttatct ttccacttct tcgttctcca
      481 ggcatctttg atcagcagcc acacatgctg gaaatttatt ttgatcgggt tccaggtttg
      541 taccggcctg gtgataccat acaccggcgg tacgttgggt aattcttcct ggtaagagcc
      601 aaaccatttg tcccagaaaa taaaaatctg tccgtagttc ttatcgatat attctttgtt
      661 gatggcatgg tgcacccggt gatgagaagg ggtcacaata attttttcca ggaaacccat
      721 cctgttgata tgccgggtat gataccagaa ctgggcaaat aaatgcagcg gtgccaccac
      781 gctgatcact gctgcgggca cacccagtaa ggcagcaggt aacaggaaga ttgcaaaagt
      841 ttttacgatg gaagaaatac tttgccgcag tgcgcaggcg aggttgaatt cttcactgct
      901 gtgatgaacg atatggccat tccaaaagaa attagccaca tgctgcaagc ggtgtaccca
      961 ataaccggca aaatccagcg caaaaaaagc gatgatataa acgataatgg tattagagat
     1021 ctgtgtcagc gccaggtgct ccaaaaaaaa cgggtaagaa agaataacga gataaagccc
     1081 gatgacatct ttggttacat tggtaacgcc gctgcttagg ctgctgatca tatccatatt
     1141 gcggacggta tcaaaacctt ttctccagcc ataccatttt tcaaaaagta cgaggagaag
     1201 aaaggccggc atggcaatga gtagtatctt tccgtaggtt tccattactg tgctaattta
     1261 cgaaagacag atggattttt ttcccgctga tttcgcagat cgtttcgcag aatatgcaga
     1321 tgataacagg gtattcaaaa atgatgtttt aattcggtgt tatctgcgtt ttcaatctgt
     1381 gcaaatctgc ggggaactaa taaagaatac tatgaaacat atatttgaat gtatagatgt
     1441 ccatacctgc ggtaatcctg ttcgcttagt gaaagagggc gggccggtat tgcatggaaa
     1501 taacatgagt gaaaagcggc aacatttttt acgagagtac gactggatca gaaccgggct
     1561 gatgtttgaa ccaagggggc atgatatgat gagtggcagc attctctatc caccacatga
     1621 cccggccaat gatgtagctg ttttatttat agaaaccagt ggctgtttac cgatgtgtgg
     1681 tcatggtact attggtgcga ttaccattgc cattgaagaa ggattaataa agccaaagaa
     1741 acccggtgtg gtgcgaatgg aggctccggc aggattggtc ttaattgact ataaggaagc
     1801 cggaagtcag aaacctgaaa taggaaggtt atcaaaagta gaaagtgtaa aactgacgaa
     1861 tgttcctgct tttcttcatt ctacagaact tttggctggt tgtcctgaat tgggagagct
     1921 agtgattgat gtagcttatg gcggaaattt ttatgccatt gtggatgtgc agaaaaattt
     1981 taagggactg gaatattata cggctgacaa gctgatcgcc tgggcaagag agttgcggaa
     2041 gaatatcaat gcaaagtatg agtttgttca cccggaagat gccactataa atggttgttc
     2101 ccatatttta tggaccggag ctgtgcttga taaaacttcc acagcaagaa atgccgtgtt
     2161 ttatggtgag aaagcaattg accgttcgcc ctgtggtacg ggtacgagtg caaggatggc
     2221 acagtggtat accaaaggga aattgaagat gggagatgaa ttcattcatg aaagcattat
     2281 cgggagtaaa tttacaggac ggatagaaga ggaattggtg gttgatggta aaccggccat
     2341 ccgtccttcg gtggaaggct gggcaaaaat ttttggatat aataccatct ggatcgataa
     2401 agaggatgac ccgtatgcat atgggttcca ggtgatctga ttaaatggcg gatcactcaa
     2461 tcaaactttt attcaacaat atgaaagcaa taatcattgg gggaggcatt atcggtttaa
     2521 gttctgcttt ttatctgcaa agatccggct gggaagtaac ggttatagat aaaggcgatt
     2581 ttctcgataa ttgttcctat ggcaatgcag gctatataag ccccagtcat tttattccat
     2641 tggctacacc gggtattgta aagaaaggat tgaaatggat gtggaattca aaaagcccct
     2701 tttatgtgca accgagattg gactggaacc tcatcaactg gggattgaaa tttatgcaaa
     2761 gtgcaaacac aaagcaggtc gaagaggccg ctgtgccatt gcgggatatt tctttactta
     2821 gtcagcagga atatgaaggc tggacaaaga taccgggctt tgattttgct tatgaacaca
     2881 aaggactact ggaaatcttt caaacacagg aaggcgcaga tcatgcacag catattttgg
     2941 agaaagctca ccagttggga ctatcagata ccgtaatact gagtaaagat gaattaagta
     3001 agctggaacc tcaaacaact ataaatgcca ttggtgcatt atggtttaaa tgtgatgcac
     3061 attgttatcc tgacaagttg atgggtagcc tgatcagtta tttaagatca gctggggtgc
     3121 agttcattgc caataatgag gtgactggtt ttggtacagg gggtggaaaa ataaaaaagg
     3181 tgcttactac aacgggatca ttcgaagcgg atgaagtaat aatagccaca ggctcctggg
     3241 gcagggagac agccaggttg ttaggaataa aaattccgct gatgcccggc cgtggctatt
     3301 cggttacatt ggaagactcc ccttataaaa taaattttcc agccgttctt gttgaaggcc
     3361 gggtggcact gacaccaatg gatggcaata agatccgttt cggaggcacc atggaaatta
     3421 catctcacca aacaccgcca cggatgaacc gggtgcaggg aatcctggat gcagtaaaaa
     3481 gatattatcc agattttgat gtggcattac cacccaaaga aaagatatgg tatggatatc
     3541 gaccctgctc tgcagatgga ttaccttata ttggccgggt gaaaaagttt gcgaacctgg
     3601 caattgccac cgggcattca atgctgggac taagtttggg agccggtacg ggtaaactgg
     3661 tcagtgaaat agttaataat aggcccacca gtatggatat aaaaccgttt tctgtggaaa
     3721 ggttttctta aatctgtttc taactatgta atcattggct gaataaatcg gtcaagtcct
     3781 aactttacaa tctcaaatga actgtacatc tactcgttta ccttaccgcc aaactggtgc
     3841 tttttcaaag cttgccatgg attatattga ccaggcagaa gcactgaaac cattctttgc
     3901 tcatcctcca acagtaaagg ggcttcagca ggcaatagag gcaagaaaac aattttcaac
     3961 aaaccgggag atactggtac aggaattaaa aaaacaatac gggggagttg agacggggga
     4021 caaagtgaaa aataatattg attcactttt atcggctgat acttttacga ttactacggc
     4081 tcatcagaat aatattttca ccgggccgct ttactttatt tataaaattg tgcatgccat
     4141 taaactggct gacactttaa aagtatcctt accgcaatac aattttgttc ctgtttttta
     4201 tatcggatct gaagatgctg accttgatga actgaatcat attcatttgg ggggtgaaaa
     4261 attagtttgg gttacgaaac aaacaggtgc agtgggaaga atgagagtgg acaaggaact
     4321 tttgaaattg attacaataa tggaaggaca attactggtt cttccgtatg gccatgaaat
     4381 tgtatcgtta ttaaaacaat tttataaaga aggagattcc atacaacagg ccacttttcg
     4441 gtttgtaaat gctttgtttg cagactatgg acttgtagtg ctgctgccgg ataatccatc
     4501 attgaaaaag ttgatggtac cggtgtttac agatgaatta ctcaaccagg ttgcttctga
     4561 tattattgaa gtgtctgctg acaaactgga aaaaggcggg tataaagtgc aggccagtgc
     4621 aagggaaatt aatttgtttt acctgaaaga tgatctgcgt aaccggattg aattgaagaa
     4681 tgaaaagtat cgtgtggtag attcatcgct tgttttttct gaagatgaga tggtaaaaga
     4741 attgcagcag catccggaac ggttcagtcc gaatgtgatc ctgcgtgggc tttatgaaga
     4801 aacgatatta cccaatattg catttatcgg gggtggggga gaaacggctt actggcttca
     4861 gttaaaagga ttatttgaac attataaagt accatatcct gtgttgatac tccggaattc
     4921 tttcctggtt cttgagcaaa aatggaagga agcggttagt aagctcaatt ttacaatgga
     4981 ggatttcttt ttgcctgaac aggaattgct caacaggctg gtggcaagag acagtaagaa
     5041 tcaaacaaaa ttgaatggct ctatgaccaa attggaggag ttatatgaat cttttaaaaa
     5101 gcaggcctca accgtagatg ccagtttgga aaaacatgtg gagtcgttga aatcaaaaac
     5161 agtttatcgt ttgcaggaat tggaaaagaa aatgttgcgg gctgaaaagc gaaaatttag
     5221 tgaccagcaa cgacagatac atactatcaa atcacattta ttccccggga atggattaca
     5281 ggaacggatc gataatatca gttattacta tgcaaaatgg gggactgact ttatcagatc
     5341 cttatacgat cattcactta cccttgagca ggaattcgtg gtactgcagg aaaagtaatt
     5401 tcactttatt tttttcttaa acagatattc agcagaaaag cctgtttttt caacgaagag
     5461 atatttgttg aatagacagt actgaccgcc gggtctttca tatcagtaaa tccggctgtg
     5521 tattttaatt caggtgagat gatcatgcct gtcctgggta ttgtgaaatc aataccgaaa
     5581 ccggcatcac ccatgaatag tgttttcttt accggcagca attctgccga ggtattgtcc
     5641 tgtcctaaaa tatattgcag ggaagggccc aatgaaaaat acggagccgc ttgtttatta
     5701 ttgaaccgga tgatcatcgg caaggcaata ttgattgccg tattcttcag gggtaccagt
     5761 tcccttataa tctctccgcc aacgatattt cttctcaggt aaaccaattc agtattgccg
     5821 aatgtcagcg tggtggcagg gcgtatcgcc aggtttttgc caatgttgat attataaatt
     5881 acaccaatat tcagtatgcc cgcacctttt gcatcgatac caatcacatt ggtgttggaa
     5941 ttatcccgga atgttggatt ggattggttg atgccgccac caaaagtgaa tccgacacta
     6001 cttttcttta ctgtattttt tttactgctt aaatttttta ccaatgctaa tgtagcagtg
     6061 gcacaaagcc atttaggagc aatacggctt gcttgttcag cataaaacaa agcactgtcc
     6121 cggcgattat tttccaggtg caatgtagca agcctgttat gtatataagc ggcatccggg
     6181 ttgatggcca atgcagcata agcatattga aacgcagttg ctgcgtcggg gtaagtaccg
     6241 ctggctttca ggaaaaacat tctgcccagt aaagaattgg caaagtcagc atcgtcttca
     6301 cgaagtatgg aaattgcttt ctccagcctg gtaccggctt cgttgttttg ctgtttttct
     6361 ttatttgttg cagtattgca atcgagatag cggttcacaa tagcctgtgc atagttgata
     6421 tattcaacag caagggtgga tttggcatca agggtatacg gattccccgg gtacttccta
     6481 tccagttgct ggtaataaaa ttcagctgaa gagccgccag taatattatt gtcttttatg
     6541 gcctggtaga atttattata agtttctgat agtgcagtat cagcatcatt aatatttctt
     6601 cttggcttta ccagggtgcc atggaatgaa tttccggggc cacgttgttg cattttcttt
     6661 gtttgcaacc atttttcaag gtaggctgtg ttgaccgtac ttattatttt ctcactgttt
     6721 tcatcacagc agaaataggg gtcttgtttc tttttaaatc tttgcatcgc aacggagggg
     6781 acatttcgtt ctacatacct ttttatttcc tggaaagtaa ctttttcatc gggtacacca
     6841 attgaatccg ccacacccga taacccgtca accaggtaat aagtaaacaa accgtgccca
     6901 ttgccaatgg aggcgtcttc cagtgattcc tggccggcgg ctgttgccag catcatgatc
     6961 tcgcctactt ttttttcagt gatggctgtg tttaaaaaac tctgtccggc agtgccaccc
     7021 ggcaattcat tggaccggca ggcatccata atgaaaaaaa cttccacccc tttggtggtt
     7081 tcgtttgcaa ttttcttttt caggttgtat aactgtatag cgccaccgac aagataatta
     7141 tttttatcac cggcagggtt gcaatcatag ctgaggaaga aaaactggtc ttcatcaatc
     7201 gcatccccat gaccggcgag ataaataaaa agtttgtcac ccttttgcag ttgttttgct
     7261 ttcagccatt gaaaaccttt ggtccagaat acagagttga tggcctgttc attcagcagg
     7321 caaaaaatat tttcttcttt cacgttgccc cccccgggtg atcttaaata gtcacggaat
     7381 agctcagcat ctttatctgc ataggttagc ggacgtatat atttgtattt ggaaatgccc
     7441 attacaatag caaaggtctg gggtcctttg atagtggtat cccttgcttt aagcggtgtt
     7501 tgcccggcca ctccggacag gaatgcagaa aataatagta agaaggcaag acccagaaat
     7561 tttctcataa atacgggttg aggttgcagg tggttaataa ggcaatagta aaatactaca
     7621 tttttcaaac ttgtgcctat ctattctttt gaaataatta cccggaacag ggtgctgctg
     7681 ccattatcgt tatctgcggc taataaaagg gtgattgaat tatctgattc tgcaagtatg
     7741 caaaccgatt ctaccttctg acctttaaaa cggtcatcca catcttcaag gtcaataatc
     7801 ttatcaggat tgattgcttt ccatcttttt ttggctgaaa tattttccac gatccataaa
     7861 taacttttac caattgttcc atcatctatg ctgtttcggg tatcctctgt tgaaacggtt
     7921 agtaatagtc tgtcgctttt gtttgaataa tccagtcctg atactccgtt gaaaacagtg
     7981 ctgtccgtat tggcacccac ccgtattagc gtgacaggtg catccgtttg gtttatccag
     8041 aaatctttcc ttgtgagtac tagaaaattt ttccggtttg ttttattacc cctgtttgac
     8101 aggatgatat taccggggat agcgcaaacc ccttcgatat ttatttcttc aagaccgtta
     8161 ttttttagcc gccggtagaa agtatccagc cggaggctgt ctttttgttt ggtgaccggg
     8221 tcaatcagcc agccggtatt gcgatagggt atagatgaac ctgagcccag tatcaatagt
     8281 ttgttggttg cagtaatggt aacacattca aggtcgggct tagtagcttt cggtatttta
     8341 tattccgtgt gcgaatagag ggtaaccgaa tcttttatca aataattgct gtcgagtata
     8401 agcaaatgct ttgcatcgtc acctgttata taaatttgtt ttttgaaata actgataccg
     8461 gaggaggaag ggtagtgggg tagttgtatc gtctcttgca gcttatgttt tttgctgcaa
     8521 gagataaaga aaaaggaaag taataaaagt agccgggtca ttatgtgttt tacatgagct
     8581 gattaaatct tatagttcat aattggtttt aatcaaactt attctcccat ccttctctct
     8641 tcatcttctc tgctttttca atcacttcgt tattcatggt atttttatag gcagcaatct
     8701 ttactgacac tgtttcatcg gcagtgccaa gaatttcagc ggctaatatg cctgcatttt
     8761 tcgcagcatt gagagccact gttcccacag gtacaccatt tggcatttgt aaaatagaaa
     8821 gtactgagtc ccatccatca atggagttgg atgatttaat aggaacgcca ataacaggta
     8881 atgtagtaac tgctgccacc ataccgggca ggtgagcggc accacctgct cctgcgataa
     8941 ttactttcaa tcctctttct ttcgcagttt gtgcatattc tatcatccgg tgcggggtgc
     9001 ggtgggctga taccacagta acctcatgcg ggatatcaaa ctgttttaaa atatctgaag
     9061 cggcctgcat gacgttcagg tcgctgtcgc ttcccattat gataccaact aaaacagttt
     9121 gtagttggtg gtttgtagtt tgtggttcac tcatccttgg ttctgttttt aaacttctgc
     9181 ccttttattt ttgatttatt caggtagtta ataaaacctg tcagcatcct ggtcagttca
     9241 tcagctaagc tatacaattc ccggaaaatc tcttctgtga tatatttatt atctaaagaa
     9301 cgatataatt gtgattttaa ttcgccggtt tctcctttag caatggttaa tgaattcgta
     9361 aactcatttt tacttcctct gtcaaaccct tccgcaatat tatccataat cgaaccacaa
     9421 ctgcccctca tttgatcttt caacctaaag tcagaagcta taggttcttt aaatgtaagc
     9481 ggataaattt tttgacttta aaaacgggct ttttgccata tctccaaatc ttcaaatttc
     9541 tggatagtgg ccataaaata aggtatgtag caaaaataac tacaaacctc aaactataaa
     9601 ctacaaacta tcttatttca ccaaatcgcc cttgaggcgc agtaattctg tttcggcaag
     9661 cttggcatta aacctggctg cgatcaaccg gttataggca ttctcaaggc tttgctgcgc
     9721 atcccttaat tgcaggatag tggaagcacc caggcgatat acttccagcg ctatgcttac
     9781 attttctttt gccagcagaa tattttcttc ttcaaggttc aatgcttttt tctgctgttc
     9841 ataatctttg tatgcattaa tgacgccaag atcaagtact gatttttgat tttcataaac
     9901 cagtcgctgg tattggatat caagctttgc ctgctgtata ttgcgcttaa ctgtaaaatt
     9961 attaaatatc ggtatggtcg cgttaaagcc aatattgcgt ccgatattcc ggctgtacaa
    10021 tggctggaaa gggtttactg ttgctttatt attattgcgc tggtaattgt aacctgcatt
    10081 taaggagagt accgggtatt tatcagctat cctttctttt aatgtgtaat tggcaatgtc
    10141 aatatttttt tcagctatta acagcaaagg gttatttttt gtggcaatat cacggatatc
    10201 aacgagtaca aggttcatgt caagcgggat ggtatcactc acatcataat ttgtattcaa
    10261 ggtcacattc atcgattgat ttaattgttc tcttaactgg gctatcaacg tttgctgtgt
    10321 aagttgcgca gctttttgtg cattcagatc cactttactc tgcaatacat caggtttggc
    10381 acctacaccg atatctaatt tatattgggc gattttaacc ctttcctggt ttaccgacat
    10441 ctgttcttcg atcgctttca gttgttgttt ttgcctgacg accgagtaat aatttgtaat
    10501 aaccgcagca atcgtattga ctacctggtt ctttatgcct aactcgccca gtttaacgaa
    10561 ttctgcaaac ttatccctgg ttgcaaacat tttcaaccca tcaaacagtg tccagttcaa
    10621 ttgcagacca aggttgtaat tattggattt gatatcatcc aattcccttt tggtaccgtc
    10681 attaaattcc tgccgctggt cattgttatt gaatgttccg ttaatgttac cgtttagtct
    10741 tggtaaaaaa gctgcattac ggtatgaata attaagcgta gctacaagtg aatcattttt
    10801 tgataactga atgtcatagt tattcttcag tgctgttgca atggcctcat ccagggtaag
    10861 taaacgctgg gctgccggtg aaaagcttat cagtactaac aggggtaaaa gaaatctctt
    10921 cataatcttc atgccaggtt tatacttgct ccactacagg ctgaattggc tgaacaggct
    10981 gtgtggctgg gtgaataaat tgttctactt catttttctt atgggcagat gaaaggaatg
    11041 agtacatcgc cgggataacg aataaggtta gtacaagtga gaacatgata ccacctacga
    11101 tcacaattcc cagcggaata cggctggtag cagctgcacc caaagataaa gccagcggta
    11161 aagcccctaa tgacatggca aggcttgtca tcaggatcgg ccggaaccgt tgcgtagcgg
    11221 agaagatagc agcttctgtt cttttcatcc ctttcaagcg gttttgattg gcaaactcca
    11281 caatgaggat gccatttttt gttactaatc caatcagcat gatcatgccg atctgggaga
    11341 aaatattcag cgtatggccg aatattgaca aggttaaaac cgcaccggca atagccaatg
    11401 gaacggtaaa catgataatc agcggatcaa taaaactttc aaactgtgct gccaggatca
    11461 ggaaaattaa aactaatgcc aggataaaag caaaactggt attgctgctg ctttctttaa
    11521 aatctcttga agagcccgaa agagaagtag caaatgtttc gtccagcact ttattcgcta
    11581 ttttttccat ttcttcaata ccatcgccaa tcgtaaagcc cggttgcagg ctggctgaaa
    11641 tggtagctga tttatacctg ttgaaatgat aaatagtagg aggagtgttg gattcgctta
    11701 ccgtaacaag attatcaaga ctgatcaatt caccccggtt attcctggcg aacaatgttt
    11761 tcaggtcatt gggatcatcc cggtcattgc gggccacctg tcccatcacc tggtattgtt
    11821 taccatcttt tgtaaaataa ccaaggcgcc ggttactgta cgccaattgc agcgtttggg
    11881 aaatatcgtt tacacttaca ccgagctgtg ctgctttcaa ccggtcaatt tccactctca
    11941 gttccggctt gttgaatttc agatccacat ctgcattcaa caatacttta ctcagccttg
    12001 cttcttccag tattttggga aggacctgcg ttaatttatc aaaattgttg ttttggataa
    12061 cgaaagcaac gggctgtccg ccacgccggt tcacggagat cgtttgttct tcaatagcaa
    12121 atgcacggcc ttcgttatat tttggtaaat ttttatttac catttttaca atttcatttt
    12181 gtgatctttc tctttcattg ggtggtatca atgtgatacg gacaaagccg gtattggcgt
    12241 ttccggaacc agaaaagccg ggtgaagtaa cactcaatac catatctttt tccggcaccg
    12301 agtcaagcat taaattggtc agcctgttta catatttgtc catggcatcg taagaagtcc
    12361 cttccggtgc tgacaactgt aaccggaact ggctccggtc ttccatcggt gccaattcgg
    12421 attgcaggtt tcggccaaca aaaaatatgg cagccaaaca aagtgcgatc aggataaagg
    12481 ccatccagcg gatagccata aactttctta aggcagcctg gtaagtattt tccatccaac
    12541 ggaaaaaagg ttctgtttta gtatatagcc agggatgttt ggaaggattc ttcctggata
    12601 attttacgtt cagcacaggt gttagtgtaa gtgatacaaa ggctgagata agaacagcac
    12661 cggctacaac aataccaaac tctctgaata atcttcccac aaaaccctgc aggaatatga
    12721 tgggaagaaa aacaactgcc agtgtgatag atgtggcaat taccgcaaaa taaatttctt
    12781 tagaaccttc cagtgctgcc tggtatttgt tcattccccg ttctatcttt ttataaatat
    12841 tctctgtcac tacaatacca tcatccacaa caagccctgt tgctaataca atggcaagta
    12901 aggaaagtac gttgatcgtg aaacccatca ggtacatgat gaagaaagcc ccaatcaggg
    12961 aaacaggaat atctattaac ggccggatgg caatgatcca gtcgcggaag aaggcataaa
    13021 tgatcattac tacgagtaag aaagcgatca tcagtgtttc ctgtacttca aggatggatt
    13081 tcttgatgaa ctttgtctgg tcaagagcaa tattgatctt tatatcttcc ggtacatctt
    13141 ttttaatctg ctccagtctt ttgaaaaatt catcagagat ggaaacgtaa ttagatcctg
    13201 gctgagggat tactgccaat gcgatcatgg ggataccatt tcctttcaaa acggtttctt
    13261 cattttcggg tccgagtact gcttcgccaa tatcgcttac ccggatatcg gcgccttctg
    13321 catttttaac gatcaggtta ttgaattctt cttccgtgtt caatctgcca aaggttctta
    13381 ccgtaagctc agtggcactg cctgatattt taccggaagg aagttctaca ttttctctta
    13441 ataaggctgc ttgcacatca ttggctgcaa tgctatacgc attcagttta gccggatcga
    13501 tccagatacg catagcatat cttttttcgc cccagatttg tatgccactt acgccgggaa
    13561 tggtttgtaa tctttctacc aggacgttgt tggcgaattc tgttatttgc agctggtttc
    13621 gggtattgct ttgcaccgtc atggaaagta tagcatcaga actggcatct gctttggaaa
    13681 ctacaggagg tgcatcaagg tcagctggta atgaacgttg cgcctgtgat actttatccc
    13741 gtacatcatt ggcggcagtt tccaggtcta ttcccaattc aaattccaca tttatattac
    13801 tgcttccatt gctgctgctg gacgtgatat tcttaatacc tgcaatcccg ttaacggctt
    13861 tttcaagcgg ttcggttatt tgtgtttcaa tgatatctgc attggcaccg ggataggaag
    13921 tccgtacact gatattagga ggatcaattg ccggataatc tcttacaccg agaaatttaa
    13981 agcctataac accaaacaat acaataatga tattcatcac tatcgccaaa accggtcttc
    14041 gcaaacttaa ttctgatata ttcatagttg aaatattgtg aatgtcttat tgagtagtga
    14101 gaaaacactt ttcaaacagg gcattcacta ttgactattg ataattttac caatttgtat
    14161 gggtgcttcc ggccttacac ttaataatcc tgttaccacg atagtatcac ctctttttaa
    14221 tccatctgtt atttgcactc ttgaagagtc ccggactcca gttgttacat cagtaaacat
    14281 ggcattgcca cttttataaa taataacttt tttccctctt gcttgtggta gtatggcctg
    14341 tgttggtatt aaaatagcgt tggtatcagg tgcgaaactt aaatggactt tagcgaaagt
    14401 gccgggaagt aaggctttgt ctttacctgt tactaaagcc ctggctgtaa ggctacgggt
    14461 gttttcggtt acgtttgact cggttgctat tatttttgca cccaattttt tatctgaacc
    14521 ttcaaatgta aatgttacta attgtcccac ttttatctgg ccggtatatt tttcaggcac
    14581 agaaaaatcc aatttcagtt ggtcagtctg attgatgact gcgatcaccg atgcagggga
    14641 aacataggcc cccgggctga tattttttaa gccaagcatg ccgctgaaag gagctcttac
    14701 ttctgttttg gcaatactgg tgcgaatgat gccgatatct gctcttaaat tatttacctg
    14761 caataaactg atatcgtagt cttgctggct gattccctga attttcagaa gctgggcttg
    14821 tcgttcttca gttttttggg ccaggcttaa ttgcacttct aattttttta actgggcctg
    14881 caggtctcca tcgtataatt tggcgagaag tgtccctttg ccaatatacc ggccttcagc
    14941 gacattcagg tatacaattc ttccggatac ttccggatga atttctgcaa cctcatttgc
    15001 tactatgtta ccgggaactt caatcgattc tccgaatgat tcagccctaa caatataacc
    15061 atccacccgc atgggagggg ttttggcacc tccaccgtgc tgaggggctt ctactttctt
    15121 tttgtttcct ccacaggctg atagcgttaa aatgaaaccg gctaataaaa atattgtaat
    15181 gattctcatg tttggtgttc tgtgcaattg gtaaaaaact ggttaataaa ggtaacagat
    15241 aaatacaagc tattggacgt caaaaagctg catttcctgc acaaaaactt ttcgaagtat
    15301 aagcggttga attggtggat aaaaggactt aagccttcgc taacaaagtt ctctttactt
    15361 tgtttgactg gtgcaagaga tcttgctttt cattactaag aatagtaaca tggcccatct
    15421 ttcttccggg cctggtttct ttcttaccgt ataagtgtac aaaagcattt tcaattttca
    15481 ggacttcttc cagtcctaca tactttacct tgccgctata gccttctgcg ccaattatat
    15541 ttaccatgat agaaggtagt atggcatcag tatttccaag aggataaccc aaaataatcc
    15601 tccacagcat atcaaattgg gaacagaaat gagcttctat agtgtgatgg ccgctgttat
    15661 gaaccctggg agctgtttca tttactaaaa catccccatt tttatcaaca aacatttcta
    15721 ccgcaaaaac gccaggggat ttaaaattcc tgactacgga taaagcaatt gcttcaaccc
    15781 tgtaaagcgt ttgggtactt aattcggcgg ggcaaagctg gtaatccagc aaattgagtt
    15841 ccggatcaaa caacatctca acaggtgggt ataaagccgt ttctcctttt tcgttaatgg
    15901 ctaccatttg ggcaatttct ttgtgaatgg ctaccatctt ttccaggatg gatggggtat
    15961 caaaccccct tgctatatgt tctttggctt ttaataattg aactccttta ccatcataac
    16021 caccttcacc aaccttatgg accgctggta aaaagtcttc agcctgtttt aattcggaca
    16081 ggctattggt gatgataaaa tcagccgttg gaatttggtg cagccggtaa tattctttct
    16141 gcaggatctt gttcttgatc gtccttaaga ctgaaggttt aggaaagact tttacccctt
    16201 cagtttctaa tttttccaat gcttcaacat tcacattttc aatctctatg gtgattgcat
    16261 ctaaattttt accaaagttg taaacagaat caaattcttt gatatctccc tttgtaaaat
    16321 gatggcagag atgtgctgcc gggcattcct tatcattctc cagcacaaag gtttcaaccg
    16381 gataattggc agcggtttgc agtaacatcc ggccaagctg gccaccgcct aaaatgccca
    16441 cttttaacat gtggcaaaga tagggtgtgg agtgctgagc gaagtagaag gactttccct
    16501 tttttattta gatttgtgtt atgatacctg actatcccca caaagtattt atcgatgaac
    16561 gcagcaactt cagtctgctg atggaggcat tggccatgga tagcccattt tgaacctgcc
    16621 tgcaaaaagg ggggataact gccggtttta ataattacct aaattctaaa ccagcctgag
    16681 gtcttcaggt ttcaacatca tgaatacaaa aacgattaca gataaagctg ctatacaaac
    16741 tgattttcta ccccttgagg gaactgatta tgtagaattt tatgtgggta atgccaaaca
    16801 ggctgctcat tattatatga gtgcttttgg tttccaggca ttggcttatg ccggtccgga
    16861 aaccggggtg aaagacaggg ccagttatgc agtccgtcag cataaactca cttttgtttt
    16921 aacaacagca ttgagaaccg ataatcccat tgctgaccat gtctacaggc atggggatgg
    16981 tgtgaaggca ttgtcgttga gagtaccgga tgccaccagt gcatgggaag agacgactaa
    17041 aaggggggct aaaagttata tggaacccac ccggctaaaa gataatgatg gcgaagtagt
    17101 gatgagtggc attcatactt atggagatac cattcattta tttattgaaa gaaaaaatta
    17161 taacggtgca tttatgccag gctacaggcc atggaataac ccttacttca aacctgcaga
    17221 tactggctta ttatatgttg atcattgtgt aggtaatgta ggctggaacc agatgaaccc
    17281 ctgggtaaaa ttttatgagg aagtgatggg gtttaaaaat atcctctcct ttgatgatga
    17341 agatatttca acagaatact ctgcgttgat gagtaaggtg atgagcagtg gaaatggttt
    17401 tgtaaaattt ccgatcaatg aacctgcaga aggtaagaaa aaaagccagg tagaagaata
    17461 cctggagttt tataacggag aaggttgtca gcatgtggca atggctacca atgatattgt
    17521 aaaaacagtt cgtgacctgc aaagccgggg agttgaattt ctgaaagttc cgacttctta
    17581 ttatgatgat ctgctggaca gggtaggaca tattgatgag gatcttgcgc cattacggga
    17641 attgggtatc ctggtagaca gggacaatga agggtacctg ttacagttgt tttcaaaacc
    17701 agttgaagac aggccaacac ttttctttga gatcatccag cgcaaagggg ccaaaagctt
    17761 tggtaaggga aacttcaaag cattatttga agccatagaa agagaacagg aggcaagggg
    17821 aaacttgtaa atacaaggta aacttacgag accggaagtc tgaaaatggg ttagatttta
    17881 tctatggcat gggtattatc ccgggctatc tgactatccg attttcggtc ttttataact
    17941 atatttacaa aagtttggct attagctgat aacaatatct ttaacctgtc gttgattccg
    18001 taagtgacta tgatgaagaa cctttttttg atgctgatat cagtaaatat tgtactggta
    18061 tcatacgggc aaccttctcc cacttcttca aaagggggga ataattctta ctcatttatt
    18121 gattatcaaa aaacatttcc caggcccggt gaagcattgg agagaaaaga agatacactg
    18181 caaaagcaat ttgaagctaa gaaattgcaa tggccggcca aatacatgta tatccgttca
    18241 tttaagtacg atagccagtt agaagtatgg gtgaaaaatg attggaaaga gcaatttcaa
    18301 ttatttaaaa cgtacaaggt ttgtgcgctg gcgggttcac tgggtcccaa gcgaatgcag
    18361 ggagattacc aggtgccgga aggcttctat tatatcaatg aatttaatcc ccgcagtaat
    18421 tattatctgt cactgggcat caattacccc aacgcatctg ataagatact tagtgattca
    18481 ttaaagcccg gtagcgagat ttatatacat ggcagctgtg ttacggtagg ttgtatccca
    18541 attacagatc agcagattga tgagttatat attcttgccg cacatgccaa agaccagggc
    18601 caggatttta taccggttca tattttcccg attcgttata atgtggagag aagtgttaaa
    18661 tacctcgaaa gcttaacaaa ggatgaccag gatttaaaga agtttacaga caagctggaa
    18721 gacgcatttg actattttga aaaatataaa cagttgcctg tagtgatgat taatgataaa
    18781 ggagaatatg tcattaatga agcagctcca aaaaaattaa aaggaatttc tactgaaatc
    18841 aagcaacagg taaaaagacc tccggcccag catcgggaaa gaaatatcgg attactggca
    18901 gaggctgtac atcaatggcc acagtatccc ggaggaggag aggcttttat gaaatacttg
    18961 caggtagtag gaaatgaaat gaccgctttt ctgccaaaag gaattaaaaa agcatttatt
    19021 caggttgagt ttattgttga taaggacggc gtgccggtaa acctgaaagt attaaaaggc
    19081 tttaaagagg gcgaagagtt taatgatgaa ctgatttcac gtctggaaca aatgggtacc
    19141 tggcaaccag ccttactgca tgaaaaacca gtagccaaaa aaatgataca aactattacg
    19201 gtagaagcac ccctggctcc ctgaaggtta tgaccttatc ctaataaaaa ttgtactgtt
    19261 tcgggagtct gctggcgaag cagtattaac tttcccattg tcagggtctc aactatttct
    19321 ttattgtaat gtcggatagt cagcaaggat aaccctttct ttacctgtac atctaaaaaa
    19381 gcagatgctt caaaagcaag tttttcaatt ttctccggcc aattatccag cacactcaaa
    19441 aagctgatag cgccattctg tgtgaggttt ggtttaatat gcagtttttc aaacaggtga
    19501 tataaatggc caacatgttg ttcgccaata aaagaaaaat ctcttgaatt catttcaatc
    19561 atcacctgtt tttcttttaa aacaataata ggaggcaggt ggtgtaccgg tttattatga
    19621 atgattgtgc ccggcagaga agggtttgaa aaacatttta catacaaggg tatgttttta
    19681 ttttgcagcg gtttgatagt cttgggatgg attacctgtg caccataata ggccatttca
    19741 atcacttcgt tgtaattcag ttcaggaatc gagatcgcat cagaaaattg tttggggtca
    19801 gcattcatca cggattccac atctttccag atggtcaggc tgtctgcatt cagcatattg
    19861 gcaaaaacag cagcagtata atcactgcct tctcttccta aagttgtgct ttcattctca
    19921 tccgtggcac cgataaatcc ctgtgttaaa acaatattat agtgtttcaa ttccggtaat
    19981 actacttcat ttactttttg tttcgtgaac tcccaatcaa ttgttgcatc cctgaaatca
    20041 tcatctgtcc tgaaaatatc acggatatcc agccatttat tatttgctcc cacctcattc
    20101 aggtaagcac ttacaatagt tgttgagaat aattcaccgg cacatactac ctggtcataa
    20161 taataatcat aatctctcac cggtttatca tgcaataacc attctacctc tgtaaaaaaa
    20221 tctttcagtt gcgcttcaca ggcaaggtag tttgttacca gaaggtattt actggtagtg
    20281 agatgttgtt gttttacttg ttcaaataac aagagagctt cctctttttt acctgcaaag
    20341 aatgcatcta caactttttc aagtgcattg gtggttttgc ccatggcaga cacgatcacc
    20401 acaatctttt cattggccca atggttcatg ataggggcaa gattctgtat tctctcaatg
    20461 gtattgactg aagcaccgcc gaatttgaag accttcatta tttttttgac aggttgataa
    20521 gtcggcaaat gtcggtaata ttccctttca acctgtcttc ctgtcaactt ttcaactccc
    20581 actcaaaacc ttacttttgc tttgttccaa aattgccaac atgtctataa acagaaagat
    20641 ttcaaccctt gatgagttca ccattcagca gctgagagat tttcccaatg ccaccggtga
    20701 attatcccgg cttttgcggg atatcggact ggccgcaaaa agagtgaatg tggaagtgaa
    20761 taaagcaggc ctggtggata tacttggcga tgccggaacg gttaatgtgc agggggagga
    20821 agttaagaaa ctggatgtat atgcaaatga ccagtttacc ggtgttttac ggcacggcat
    20881 cagttgtgcc ggtattgcaa gcgaagaact ggatgatttt gttgcttttg atgatgagat
    20941 cagcaaaaag tcaaaatatg tttgcctcta tgatcctttg gatggcagtg ccaatattga
    21001 tgtaaatgtc tctatcggta ccatattttg tgtgtataaa agagtatcac cgatcggaac
    21061 gcctgtaacc cttgaagatt ttctgcagcc gggtaaccag caggtcgcag ctggttatat
    21121 gatatatggc tcttccacta tgctggtgta tgcaacccgc agaggagtaa atgggtttac
    21181 gcttgatcct tctatcggtg aatggacact cagccatgct gatattaaat gcccggagct
    21241 gggtaagatt tattctgtaa atcacggcaa cttttttaaa tatgaggaag gtgtaaaaaa
    21301 atatattaca gcctgtcaga aaaaagataa gaccaatggt ggcccatata cccaacgcta
    21361 tattggtagt atggtggcag atgtacaccg caacctgatt aaaggcggta tttttaccta
    21421 tcccggtact accgataagc caaagggaaa actccggtta ttgtatgaat gcaatccttt
    21481 tgcttttatt gtagaggtag caggtggaaa agctacagac gggaaaaaaa ggatactgga
    21541 tattcaaccc actgagctgc atcagcgtac accatttttt gttggcagca agttgatgat
    21601 ggaagaactg gaaacttatc taaaaccgga ataataatgg ccaagacaat catatctgta
    21661 aaaaatctcg ttaagaatta cggcaatttc caggcagtaa aagggattac attcgatgta
    21721 atggaagggg agatttttgg attgctcggt ccgaatggtg ccggtaaatc aactacactt
    21781 gaaattattg aaacactaag gcccaaaaca agtggcgaaa taattgtgga tggcttgtgt
    21841 ctcgataagc aaccaaacga tattaaaaaa ataattggtg tccagttgca aacaagtggt
    21901 tattatccgg gattgaacct tttgcaattg attgaattat tctgcggtct ctataacagg
    21961 gaggttgacc caatggaatt actggagatg gtgaatctga aagacaaagc aaagtcacca
    22021 gtaaaagaac taagtggcgg gcagaaacag cggttttcag tagcgaccac tcttattaat
    22081 gaaccaagga tcattttcct ggacgaaccg actacaggac ttgatccgca ggcccgcagg
    22141 agtttgtggg agttggtcag gaatatcagg gcaagaggaa caacagtaat cattacgact
    22201 cactatatgg acgaagcaga atatctctgc gacagggtgg ctatcatcga tgcgggaaaa
    22261 ataattgctc tggattctcc tgataaactg gttgataacc tggtggcgtc cggatttgaa
    22321 agaccaaaag aaatgaaaat ggctaattta gaagatgtat ttattagcct gacaggccat
    22381 tcattacggg aaggataata attatgagta ttcaaaataa actaagggaa tttatgcaag
    22441 gaaaaattga agcacaaaaa actgcaggtg cagcttttat gagtgaaaat aaactgaaag
    22501 aaggagtaac cgaattaccg gaagggatac aatatgaagt gataaaagaa ggtagcggcc
    22561 accagcctct tttgaaagac acagttaagg cacactataa ggggtcctta ctggatggga
    22621 aagagtttga taattcattc aaaagagggc aacctttttc tgcaccgctt actgcattga
    22681 taaaaggatg gcagattgca ttgcctttaa tgaaagaggg cagccattgg cggttgtgga
    22741 taccatcaca cctggcttat ggagacaggg gtgcgggcag tgatattcca ggtggagcta
    22801 cacttatttt tgaagtggag ttaattgaaa ttgtaaaaaa ataaatttca gcttatgcct
    22861 gttactgcag ttgatccccg ctatccgatt ggagagtatg ctccccaacc tttttctatc
    22921 ccccggaaag tagaatggct ggctgatatt aagttcttac cactgcaatt ggaaaatgcg
    22981 gtattgaatc tcgatgaagc acaattgcat actgcttaca gggatggtgg ttggacagta
    23041 cagcaggtaa tacatcatgt agctgacagt catatcaatg cttattgtcg tttcaagctg
    23101 gcattaactg aagacaaccc aacagttaaa ccttatgaag aaaaattatg ggctgaaatg
    23161 aatgatgttc agaagttgcc tgtcaatata tcattaaccc tgttacatgc cctgcatgcc
    23221 cgttggtatg aatcattgaa atatgtttca gataacgatt ggaataatcg taccatttat
    23281 catcctgagc aaaaaaagac catccgctta tggacattgc tggggatgta tgcctggcat
    23341 ggcaagcatc atgttgcgca tattacttcg ctcctggaaa gaaaaggatg gtgatcgcag
    23401 tacacagcgc ataaacatac tatcaacgat ctacaaataa tagtgaaatc ctcatcatga
    23461 aatggaaaac gctgtcgtcc gaatatcttt ttaatgaccg gtggtttaaa gtgcgcaggg
    23521 ataaatgcga aacacccggt ggtaagattg ttgatcccta ttatgtgtac gaattttcta
    23581 cctgggtggg tgctttaccg gtgacagaag agggtaagat aattatggtt cggcaatacc
    23641 gtcatgcact tggtgaaaca tgcctggaaa tccccggtgg ttgtgtggat gatactgata
    23701 cgaatgtaga atatgccatt gcaagggaac tattggaaga aacaggttac tctttcacct
    23761 catttgatta cctgggaaaa atttctccaa acccctccac taattcaaac ctgctgcata
    23821 tgtttctcgc aaagggaggt aaaaaggtgg gtgaacaaaa tcttgatgag aacgaagaga
    23881 ttgaagtggt ggaaatgacg cttgatgaat taaagcaact gttaagggag aacaagatcg
    23941 ttcaggcaat gcatgtgagt tgtattttat atgcgttgga aaaattagga gagctccact
    24001 attaatcgaa aaacctgatt aattcaagaa agtcaaaatc aacgaaagag gagttctctt
    24061 ttccccatat ttctttaaca ctccttaagc ttacttcgcc taccaggaat atgcttccgc
    24121 atacgatgat aaggtctttt ttaccagctt tgtgagaagc gctgtaaagt gaatagttga
    24181 cattcataaa tggtttacct tttaatccga atgaagcggc tttccgggct aactcttctt
    24241 ccggcaaagc tcttggtatt tgcgcctttg taaaataata agtagcatct tttggtaaaa
    24301 gttccagaac tttttctgcc tctttatctt tgaccatgcc taaaattata tggaggtgat
    24361 ggtgttcagt taattcaatc tgcctgacta attgttttat tccatccaca ttatgtgcca
    24421 catctaaaat tatgagagga tgatgatgta tgacctccca tcggccatgc aagccggtta
    24481 ttttttttgt ttgttttaaa gcatcagtaa ttgtctgagc aggaataatc catcctttgt
    24541 tttgtaattg gtaacaggtc tctaaaactg ttaacaggtt ttttgtttga taaatacctg
    24601 gcaggtcaag gtgatatatt ttatggtctg ttttgtgttt ttcggccact tctacaatga
    24661 gttcattttt ttcccaattc cagtctgcgg tttgtcttgt atctgaaaca atatgaatcg
    24721 ctgatttttt ttcttttgcc acctgctcaa aaacgggcaa tgtttctggc agaacttcgc
    24781 caatgattac cggtactccg ggctttatta ttccagcttt ttcaaacgca attttttcaa
    24841 ggctgtcgcc cagcatgttc atgtggtccc agccgatatt ggtaatcaca gctaactcag
    24901 gagtaatgat attggtactg tccagtcttc cacccaggcc ggtttcaata atggcaatat
    24961 caactttttg ttgtgcaaaa taatcaaagg ccatagccac ggtgatttca aaaaagctgg
    25021 gctctatttt ttcgattaat gggtttatgg tttctgtaaa atcaatgact gcctgctccg
    25081 gaatcatttc gccatttatt tttatcctct ccctgaaatc tttcaggtgc ggagacgtgt
    25141 aaagcccggt tttatatcct gctgtttgta agacggccgc cagcatatgg cttactgacc
    25201 ctttaccatt ggtaccggca atatggaccg ttttgaattg gtgatgagga tttcccaggt
    25261 gattacaaag ctcaattgta ttggtcaggt cttttttaaa cgcagccgcg ccaatacggc
    25321 tgaacatggg gagacgggta aataaatagt ctatggtttg ctgaaacgtc attccccgca
    25381 aatgtaggat atgccataaa taaaaaacca ttccgcatag ccgaaatggt ttttaaacaa
    25441 ttataattta gttagttaac cctgaagtta aattgaacag tgatcatgga ttcatgatct
    25501 gctttgtcaa acttgatatt ttgcaggtaa cgtataatag cttctttgta agcattactg
    25561 ctgttggaag aacctttagc aatacttacg aatttgccga taccatcagg tgatacactc
    25621 acatttgcat aaattgttgc tttatctaga tcgccttcaa atgaataact acgaacaatt
    25681 ttcctgtcgc ccctggtaac ccgtggacca ttaccaacac cagttccgga accattgcca
    25741 cctcctatac cattgccatt tcccccacca acaccgctgc cgttaccaac accataacca
    25801 gttccttttc ccccggcctg gtcgtaatta tcagcattat ttccaccttt ttgagtaccg
    25861 gtaatattct ttccatccat tactgcctta ggtcttggtg gggccggagt ttttatttct
    25921 ttaggctccg tttttgtaat agaagtattt gtgatagctg ttgcttttgg ttttgttgct
    25981 acaggcttat tgatggcagg actggtgcta ttattatcct cttcaatgtc tttggaatct
    26041 tcagtagtac ccggaggcat tggcgctgcc tgtgctattc cgggattacc tgccgcttgc
    26101 acaggattgc cgccaccgcc accaccttca tcaaatggaa caggagggtc aggaggcagg
    26161 ttaagctcca cttcaattcc cggtaactcc acaatctttt caaatacagg aagcttccac
    26221 ttcaggagga acatgagcaa tatggctaaa ccggcaaaac cagctgtaat catagcggct
    26281 cttgaacggt attgggcttc aagggatgca gacattgtgt aagcgttgtt catacaaatt
    26341 taagctttga ttatcaatgg acagtgtagt tgtttattca cacctgttat taacgatgca
    26401 aacgctgtgc aacttgcata aaaggggatt tataaaatgt taagattatc agaaggattg
    26461 ttcctacttc ctgagattgg caattaagag gcccaaaatt accagtacaa agcttaccag
    26521 gtgtacccaa ttgatttttt cacctaaaat caacatagct tccatagtac tgaaaaccgg
    26581 gatcaggttt ccgaataagg cagctcttcc ggcacctaga ttccggatgg caatattcca
    26641 tatccagaaa caaatgactg atgcgccaag gcccaggtaa agtatgatca gcaaggcaga
    26701 tacattccag gaaatgggcg cagcatgtgc tgtttcctgt atataaaatg ggactaacag
    26761 caatgtcccc atactgaaca cgatgaaaag aaaattgaca ggagaaacag ctgctggttt
    26821 cttttttacc agggtattgt aaactgcaaa ggcgaaacca gccagtaaaa cccagctatc
    26881 accttttgta aatttcaact gtaacaggtt ttgaaaatta ccttttgaga gtaagaataa
    26941 tataccggca atacaaatag taataccaaa aacccgggta ccacttattt tctcattcaa
    27001 aaaaatacgg gcaaagatga ccgacatgat gggagaggag gttgttccga ttaaagcaag
    27061 attaattgcc gttgtgtatt gggcccctac atacacaaat gtattgaaca tggcgatgcc
    27121 ggttgctgca gcaaatagaa agtagatcca tgattgttta ataacaggcc attctttttt
    27181 taaatactta aaggcaaaag gaaaaataag tatagtggca gttaaccagc gaaagaatgc
    27241 caggcttacc ggcgggattt ctttatagat acctcttgca acaataaaat tccaggacca
    27301 gataaaagtg gccaaaacag caaggccaat tccggtataa atattttttg cagccgaatg
    27361 gttcattaaa atttagtagt gtgcaaaatc catatcaaac tttcctttga tcctttccat
    27421 cggcggattg aaataaccga attttaaatc tggaaattct tcccggatca gttcctgcat
    27481 ttgttttaca cccatacatt cgccggtatc agttactcct attttgccaa gataccagtc
    27541 tggatcaata ttaaaattgc gccactggat cattttcaga ccggtttctt tgatcagttt
    27601 tctcaaggct tcatattcag ctatggagtc tgtcattccc gggaaaacga aatagttaat
    27661 ggaggtccag ccgccatacg agttgacaac ttttaagctc tcgattatat catcgaactc
    27721 gtaattatta gggcgatagt aaggcatgta aatctctctc ctggcggaat tggtacttac
    27781 ccgaatcgaa tccaggcctg cttcacagag aattttaact gcttcagggt ttgagccatt
    27841 ggtattgata ttaatgctgc ctttcttagt atgctttctt atttcaatga tggcatcacg
    27901 gatggtttcc cacatcagca gcggctcacc ttcacaacct tgcccgaaac tgataatagg
    27961 aaaaggagct gattccaggt gtggtactgt aaattcaaca atctcttcgg gtgttggctt
    28021 aaagttcagc ctgtcttgcg tggatacgat ctgttcttca tctggctgca atgaaataca
    28081 accgacacaa ttcgcattgc aggccggaga agctggtacc gggcattccc atcttcccaa
    28141 tgcgaaattc cttgctgcag ggcaactata agtcaatgca caattattca tcaggtgtgc
    28201 cactaacctg ttgtgcggat aatttttcag taattcttgg gcaccggttt ctactctttt
    28261 tgcatcataa ccggcacatt cctgccttat atcaggttcg atccgtatag ccggtacata
    28321 aaatttttca tctaaccagc cggctgctgt atagcaaaac agcggaagga taggtgcttc
    28381 tggggc
//
LOCUS       DAORTI010000035        28325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1223_length_28325_cov_7.687266, whole genome shotgun sequence.
ACCESSION   DAORTI010000035 DAORTI010000000
VERSION     DAORTI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 28325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28325
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_28325_cov_7.687266"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..2005
                     /locus_tag="PLO70_06535"
     CDS             203..2005
                     /locus_tag="PLO70_06535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQZ74153.1"
                     /translation="MRLRIIFLFIQLIFCELYLKAQSPTYPKEYFRNPMGIPIDLSAN
                     FGELRPSHWHMGLDIRTNQKENLLVYAAAQGYIAKIGIRSQSFGRFIVINHPNGLSTL
                     YGHLNDFYPELEKYVTAQQYKQESWAVELDLSKDKFPVSKGQFIAYSGNTGGSQGPHL
                     HFEIFDTKSTKRLNPLLFDFGIRDNVPPAIVKLAVYDRGRSVYEQSPLLIALKNTDSG
                     YIIPKFPVMKTGFSKLSFAIQAYDRVSGSTNPNGIYAAKLFVDEQPQIAFVLDSLDYN
                     ETVYLNAHVDYKHKHNGGAWLQHLSKLPGDAGAAYKKFNGDGVISVTDTAIHDIRIEV
                     KDAKGNMSKLNFRLQYIDSLAKPSINQEGSIYFAPNSKTVLAKPDFEIHLPGGSLYDT
                     MKAYYFRNNTAVAKNAISALHQVNDASVPVHNNFTVFIKLYKPVLAEWSDKLLIQRSY
                     RNSNTVRKAVLKGEWITTSFDDFGSFQAFVDTIPPRISDLKNDDTINLSASSRIVFTP
                     TDNFGVITNFRAELNGQWLRFTNDKTRNWIYKFDERCPYGVHELKVTAGDLAGNITMK
                     TWWFKRGPYTPPPKKKVMKKKSSTKRKTTALKKK"
     gene            2022..2483
                     /gene="bcp"
                     /locus_tag="PLO70_06540"
     CDS             2022..2483
                     /gene="bcp"
                     /locus_tag="PLO70_06540"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQZ74154.1"
                     /translation="MIKLKEGDKAPVFTGNDQDGRKVSLADFKGKKVVLYFYPQDMTP
                     TCTVQACNLRDNFSMLKKKGFEVIGVSPDDVAKHKKFEQRNKLPFTLIADISHKILDK
                     YGVWDQKQLFGHKYMGVLRTTFVIDEKGIICKIFLKPKSKSHAEEIMTAVK"
     gene            2582..3493
                     /locus_tag="PLO70_06545"
     CDS             2582..3493
                     /locus_tag="PLO70_06545"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HQZ74155.1"
                     /translation="MKQLFTLAVFFISFIVAAQDSAIKITAGKPVRIFNSTKTINART
                     TEVTGKGKMDFNVTHNFGDIAGDNGGIKRFFGLDNAADIRIGFHIGMGHKTDLVLARA
                     KGASQVQSLWEIGVKHQLLIQKQDDPTHPLSLAIYANAVISSQKRSPFENQDNSFTGL
                     ADRTSNVVQLIIARKINKISLQLNPTFMTRGYAVSYDQKSMFALGGAIKLPLVANRLN
                     LVVDYFHPFRKQATKDSFAVNENIRFFDPLGVGFEILTSGHIFRLNFTNATEIVENRF
                     IPRTITSWGKGQFRWGFTISRNFTLWR"
     gene            3600..3917
                     /locus_tag="PLO70_06550"
     CDS             3600..3917
                     /locus_tag="PLO70_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HQZ74156.1"
                     /translation="MQVQIKEKSWVAYLAAAKLKADKVAIVFGSTIHLHNTSREEFLK
                     DKDWICHELKHVEQYLQHGYIGFILKYLFEWIKKGYYNNKFEVEARQSENEISLLQKA
                     NIT"
     gene            complement(3914..4687)
                     /locus_tag="PLO70_06555"
     CDS             complement(3914..4687)
                     /locus_tag="PLO70_06555"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQZ74157.1"
                     /translation="MPQPFSTTPVGLPFIELQSVDSTNNYARAQIHAGLAQHGMTIFA
                     HEQLAGKGQRGKVWTSEKEANIALSVVIKPASLRLTQQFHLSACVAVAVHEFFMNYTG
                     DDTKIKWPNDLYWQDRKAGGILIENIVGSSSSGAGNWDWSIIGIGININQTVFPPDLP
                     NPVSLRQITGKIFEPAELAKDICTYIEKYYHLLIKEGFHSIYSNYISNLYKKEEKVKL
                     RKENRVFEAVIKKVTPAGKLVVQHAIEEEFDFGELEWMI"
     gene            4744..5169
                     /gene="rsfS"
                     /locus_tag="PLO70_06560"
     CDS             4744..5169
                     /gene="rsfS"
                     /locus_tag="PLO70_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQZ74158.1"
                     /translation="MEQLAILTNRKKKSLSEGQPGAKRLTRNSKIIKTIIAAIQEKKG
                     ENIVSLDLRKINEAVADFFIVCEAGNQPQIRAIADNVQYKVKEKCEENPFHHEGYNNL
                     HWVLIDYVNVVVHVLLPENRKFYRLEEMWSDAVSQEHNE"
     gene            5243..7348
                     /gene="ftsH"
                     /locus_tag="PLO70_06565"
     CDS             5243..7348
                     /gene="ftsH"
                     /locus_tag="PLO70_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQZ74159.1"
                     /translation="MSQESNKRNETKPPRFNNRPGGGEDPQAPKKGPRFSIYWVYAII
                     FAVLIGFQLFGPLTPNMAKTNELELKNMIAKGEVEKYMIIDNRKVVRVYLTAAGIKSN
                     EVKLKEVSGKIPEKGPHMSFKITSGDAFKKEMSDYYKENPTVQQVVFDVDNERDWFGG
                     ILQFLLPVLLFVGIWILLMRKMGGGAGGGGGPGGIFSIGKSKATLFDKGTKVNITFAD
                     VAGLDEAKVEVMEIVDFLKNPKKYTNLGGKIPKGALLIGPPGTGKTLLAKAVAGEAQV
                     PFFSLSGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGKNAMMSND
                     ERESTLNQLLVEMDGFGTDVGIIVLAATNRPDVLDTALLRPGRFDRQITIDKPDLVGR
                     EAIFKVHLKPIKISKTLDIHKLAEQTPGFAGADIANVCNEAALIAARKNKEAVDMSDF
                     QDAVDRVIGGLEKKNKIISPEEKKIIAYHEAGHAICGWYLEHAYPLLKVTIVPRGTAA
                     LGYAQYTPKEQYLYNIDQLTDQICMTLGGRASEEIFFNKISTGAQNDLQQITRIAYSM
                     VTVYGMNDKVGNVSFYDPQQETSFTKPYSDETAKLIDNEVRKLIDDAYEKTKDLLTVK
                     KADVEKLANALLEREVLFQSDVEALIGKRPFEEKKPLVDEVTVEEPVEEAIPVAEPKK
                     EEEKSTDEQAGNIGLAPSI"
     gene            7358..7993
                     /locus_tag="PLO70_06570"
     CDS             7358..7993
                     /locus_tag="PLO70_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HQZ74160.1"
                     /translation="MNVSPSKENILKKIRKALSHSTPLPFPQSEGTNSVFEPLQQEAE
                     VEFAEQFTKLQGKFIYCINRQELAFQLGSLVKKQDWQKVYCVEDKLIEPVALQLSDRL
                     IKTGLENCDVSITGCEYLVARTGSIVMSTAQSSGRSTSVYAPIHICIAFTNQLVYDLK
                     DALQAAKDKYGNNLPSLITFATGPSRTADIEKTLVVGVHGPKEVYLFLVES"
     gene            8021..8452
                     /locus_tag="PLO70_06575"
     CDS             8021..8452
                     /locus_tag="PLO70_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HQZ74161.1"
                     /translation="MTSSGIYKLFVYGSLRSGFHSHVYEYISRYFNLVGDAKVKGKLF
                     DMGSYPAGIPAEEEVFIVGELYQIKHENEFSWAFGQLDDYEGVNVEEDEMQLYRREIT
                     DVICNGQVTKAWIYWFNGEVSGRPVIASGDMIEYLNQKKSS"
     gene            8449..8982
                     /locus_tag="PLO70_06580"
     CDS             8449..8982
                     /locus_tag="PLO70_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74162.1"
                     /translation="MKLPASILLFLSCVFLLSSCEFNCSVGQKDKNSSAVSREDEPRI
                     YNNINLKATGLKVEKAYLIFENGDGVPENNAVDFTSPVKMIVKIDTGWVVKNDKVMIG
                     ASEKIISEDGTVLLDKPDLFADDSGVSPQDAKAISIWAQIRLREGSPPASFTISFRIW
                     DKNGKGTIEGNYKLYSK"
     gene            9017..9787
                     /locus_tag="PLO70_06585"
     CDS             9017..9787
                     /locus_tag="PLO70_06585"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQZ74163.1"
                     /translation="MAKKIYFLSDFHLGAPDHASSLEREKIIVQFLDEIKQEAAEIFL
                     VGDMFDFWYEYRKVVPKGFVRLLGKLGELADAGISMHFFVGNHDMWMRDYFQQEFNMP
                     VYFQPKEFERSGKKFLIGHGDGLGPGDHGYKRLKKVFRNPVSKWLFGIFPPLLGMGLA
                     NYLSRRSRAQTGASEEIFLGEDKEWLIIYCKEVLVQQKINFFVFGHRHLAIEYRLNEE
                     SKYINLGDWIRYYTYAVFDGSTLELKSYKGNDDKIIRK"
     gene            9788..10618
                     /locus_tag="PLO70_06590"
     CDS             9788..10618
                     /locus_tag="PLO70_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74164.1"
                     /translation="MKKFSAILFVSFLLSATGFSQADTTFQLLKILKGDIVDFTVDNL
                     DNIYVLNSRNQVKKYTANGDSVAIYNDIRKFGRATLIDVSNPLKVLLYYRDFATVVML
                     DRFLNAVNTVDIRKQNIFQAKVIAQSYDNKIWVYDELESKLKKIDEDGKLLQETPDFR
                     LLLGYAPSPYKIFDENKYVYLYDSLKGVYVFDYFGSLKNNIMIQRWQNLKVTGKFIFG
                     SKQDTLFRYDINTFMYDEWKIPEQITGARSFNFSASRLYALRKECDGKLDCISIYAIR
                     "
     gene            10669..11751
                     /locus_tag="PLO70_06595"
     CDS             10669..11751
                     /locus_tag="PLO70_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQZ74165.1"
                     /translation="MNDFLEQVWWDNPVRNYLIAGGIILFVWLFMRYISRYLAGLLFR
                     IVHRIWNNVDKQSFTSLVMKPLGFFLLILVSIAALYKLNFPEIINVTIYKYTAKEIVH
                     CVGTIVLIVSFIGLLLRIIDFIAVLLEKKANLTPDQSDNQLIVFFKDFFKVILVIIGI
                     MMVLKYAFDLNIGGLLTGLSIVGAAIALALRESLENLIASFIIFFDKPFIMGDLVKVN
                     NITGSVERIGLRSTRIRTDQKTYVTVPNKQMVDSILDNLSLRTQRRGDLKLEIGLQTA
                     PAAIEQLLAGINTIVGRKEVENANVLLSDITSNAIVILSDYYTAPITIQEFNQVKQEI
                     NLQSLQLLGELKIEIAGSSTDVRISK"
     gene            complement(11753..12274)
                     /locus_tag="PLO70_06600"
     CDS             complement(11753..12274)
                     /locus_tag="PLO70_06600"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQZ74166.1"
                     /translation="MRKFIPVIFAFIFTTGVFAQNQFETLVERPNEKSLKGIISRSVL
                     ESDTSFKWFATNYKAFSPNTAAIAALKQQADSIQLLVFMGTWCEDSHFIIPKFYALTD
                     AAGFSQDRISLIGVDRNKKTLSHLTEALNVINVPTIIVLKNGKEAGRVVEYGKYGMFD
                     KELGEIINSLNKQ"
     gene            complement(12412..12975)
                     /locus_tag="PLO70_06605"
     CDS             complement(12412..12975)
                     /locus_tag="PLO70_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQZ74167.1"
                     /translation="MTKGLTQITFDFGAVEETEQKPVVITSTVKEEVPTIELAEPEIP
                     VLSTIVLEEKKATAGKRGRRSLKDIAVNADLVHVPEDDILFKKQYYSIGEVAAMFREN
                     QSLIRYWETEFDILQPRKNRKGDRFFRPVDIKNLVMIYDLLRRRKFTIEGAKDYLKGN
                     KRAEEKFAMIQSLQKIKGFLMELKANL"
     gene            complement(13092..14063)
                     /locus_tag="PLO70_06610"
     CDS             complement(13092..14063)
                     /locus_tag="PLO70_06610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQZ74168.1"
                     /translation="MKKIKYYYNTNTLRYEKLETPMRVKLLRVFGFLAAALVTAGLIS
                     YAAFQFIGSPKERILQQQNKNLKDNYYDLLDELKSVQQQMKELEKRDNDVYRAIFEAN
                     PIPDSARAKEMEIKLEIAKVEKLRDHQLVSSINNTLNNLVSRINAQKNSYDEVDDLVK
                     NKEKLLARTPAIQPVSNKDLNRIASGYGYRIDPVYKTTKFHAGLDFSAPQGTPIYATA
                     DGTVSTAGNSGNGYGNHVIINHGYGYETLYGHMVRVKARNGQAVKRGEVIGWVGSTGK
                     STGPHCHYEVHKNGQKIDPIYFFYNDLTPEQFDLILKKAAASNQSLD"
     gene            14192..>15214
                     /locus_tag="PLO70_06615"
     CDS             14192..>15214
                     /locus_tag="PLO70_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQZ74169.1"
                     /translation="MMTSADIRQAFLDFFRSKGHEIVPSAPIVVKNDPTLMFTNAGMN
                     QFKDYFLGNKPAPFKRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYF
                     KDEAIEWSWELLTVVYNIPKDRLYATVFEGDVKEGIPASSIALAKWKSLLPDEHIVFG
                     NKKDNFWEMGETGPCGPCSEIHVDCRPDEERKKIPGHTLVNKDHPQVIEIWNNVFIQF
                     NRKKDGSLEPLPATHVDTGMGLERLVRVLQGKTSNYDTDIFTGTIAETEKITGKKYDF
                     SDSKTAIAFRVIADHIRAISFTIADGQLPSNTGAGYVIRRILRRAVRYYYSYLDYKQP
                     LLTQLVP"
     assembly_gap    15215..15314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15315..16384
                     /locus_tag="PLO70_06620"
     CDS             <15315..16384
                     /locus_tag="PLO70_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQZ74170.1"
                     /translation="EVEVVDTKKDNDLIIHFTESIPADLSGEVTAKVDINKRKNTALH
                     HSATHLLHAALRKVLGTHVAQKGSLVNEEHLRFDFSHFAKVTEEEIAAIEALVNEKIR
                     ENIPVIIKSMPKEEAMKLGAMALFGEKYGDVVRVVVIDPNYSVELCGGTHVGATGELG
                     LFKLTSESAVAAGVRRVEAVCGKQAEEYVNEQLNLINSLRETLKNPKDIQKAIGNLLG
                     ENAGLKKHMEAMEARQLVGIRNELLQKDEIINGINFIGDIIEVSNADALKKLCFDLKN
                     HLRDHVAVLCANIGGKPFVAVGISETVAGAKNMDAGKIIKEHIAPLIKGGGGGQKTLA
                     TAGGQEAGKLKEVIEAVKKLL"
     gene            complement(16389..16532)
                     /locus_tag="PLO70_06625"
     CDS             complement(16389..16532)
                     /locus_tag="PLO70_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74171.1"
                     /translation="MEYFISVTIILICIGVIILALKIYKENRELEKQQKKYRDDEDFP
                     FRR"
     gene            16634..17149
                     /locus_tag="PLO70_06630"
     CDS             16634..17149
                     /locus_tag="PLO70_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74172.1"
                     /translation="MNVILETDRLLLRTFTTDDARLIYELNADPDVTLYTGDPVSDLS
                     HAQQVLDQVILPQYALYNHGRWAVHVKPTLEFIGWCGLKNRPERNEIDLGYRYLKSAW
                     GKGYATEAAFACIKYGFEKLGLYRIVGRAMPGNVASLRVLEKCQMTYIGEEIVDGHPA
                     KTYESINPLIL"
     gene            17318..18334
                     /locus_tag="PLO70_06635"
     CDS             17318..18334
                     /locus_tag="PLO70_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQZ74173.1"
                     /translation="MTWIQSSVLAVTIALMAPVVVLAQKDEKDKVKKDVQQIIITRSG
                     EKDDKVVVEINGDKVTVNGKPIDEFKDKDVKVRVNRLRDSEAFAYPRSPRGGTWNFNE
                     DDNVRFFNTDANRAMLGVTTEKDEKGAKVNDITKESAAEKIGLKEGDIITKIDDQKIE
                     SPDDLSEAITKHKPGEKVTVTWWRDNKEQKATAELTKWKGVNVFGSTMPGQQFKMDMG
                     DMNFENILPRLQGLQGLREPFAPGRPGAGPKLGLSVQDTDDGKGVKVIEVDDESNAAK
                     AGIKENDVITDVDGKAINSTDDMVKVIRESKEKVSVMIKLQRSGKTQNIEVKMPRKIK
                     TADL"
     gene            18508..19956
                     /gene="gatB"
                     /locus_tag="PLO70_06640"
     CDS             18508..19956
                     /gene="gatB"
                     /locus_tag="PLO70_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQZ74174.1"
                     /translation="MENTSLYEIVVGLEVHAQLLTKSKLFCGDSIAYGAEPNTHVSPV
                     TLGHPGTLPKMNIKAIEFAIKMGLACQSEIEKHNYFARKNYFYPDLPKGYQVSQHTTP
                     ICKGGFVNIKTQEGGKQIRLNRIHIEEDAGKSLHDVDEANTCIDYNRAGTPLIEIVTE
                     PDLRSADEAFAYLTEVRKLVRYLQICDGNMEEGSFRCDANISVRKKGDIQLGTKVEVK
                     NLNSIRNVKRAIESEAKRLINILETGGTVKQQTRSFDANTGNTFSIRDKEDADDYRYF
                     AEPDLTPFHIQDEFIERIRKSIPVLQEERIRHYITVLSLSEYDAAVLTEEKEMSDYFD
                     TVIQHTDNYKGAANWMLGPVKSWLNDNNKEPAEFPLPAKQLASVITLIDAGKISFSAA
                     ATKLFPALLKNPEKDPEQIALEESLIQQSDISSLEPIVDSVLEKFADKVKEYKKGKKG
                     LLSLFVGEVMKQTKGKADPRLTNELLLEKLKS"
     gene            19959..21134
                     /locus_tag="PLO70_06645"
     CDS             19959..21134
                     /locus_tag="PLO70_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ74175.1"
                     /translation="MKKLVVVFTAVIFLAACKNKSSVSKFEVKGTVVNSTAKMIYLEE
                     IPMATMQAVVVDSALIGKEGKYALKTVASEARVYNLRLDQSTYPVAAVINDVPSITLD
                     ATFSKENNQFVEGYEVKGSEISQQLKEFMVSFNTKLQAVFYIARQADSLNKIQAPDSV
                     FRKLEAENAIVAGDLKTLTSDALKKSTNPAFTMLILGYYQSTANNQGFGLQPFANEEV
                     TKLVNELAVKFPAHQGVASVKNMLQAQANKMQGMIGQTAPEITLPDPNGKEIKLSSFR
                     GKYVLVDFWASWCKPCREENPTVVKAYNKFKDKNFTILGVSLDRPGQKAEWMKAVMKD
                     NLTWTQVSDLLFWNSPVVQLYNIEGIPYNVLVDPQGKIIAESLRGSALEAKLGEVLN"
     gene            21188..23122
                     /locus_tag="PLO70_06650"
     CDS             21188..23122
                     /locus_tag="PLO70_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74176.1"
                     /translation="MRLNFFVRRILFSGLLFIQTSGWGGQFMGGEISYRFISHSGNEY
                     VYELTFKIYRQCFNPADLDNPVIKLINSTDLTRNIHPIIYREIILDNPTITTLPYTYK
                     NYCSINDPTSCIELRTYTVQVGLPHSPEGYTFYYTGCCRNGLSNLVSDAGNGGAEYVN
                     TIPVPGQALTYVTYIPSHDSVAQNSSPVAVNDSIVYACKGKPFRYQFQFFDADGDSLV
                     IKPADSFQKTNAATTNFFPIFFKPGYSNNSPMGNTIALNEEGILSGTPGVEGNFAIAL
                     TIEEYRNGILINKSRKDYQVNVAACEIKKPLDVVNCDDNIAMFLHNNNPGNIYHWDFG
                     VTNIISDTSDDTEPIYLYPQNGTFQVQLVVTNPNGGCKDSVLVIAKVYAGGLLVDFDW
                     SGQTCAGKPLTFTDRTTSTAGAVAENWTWRILNNNTTIGNTPSVTYTHAVSGTMPYPL
                     AVRLVVETDLGCKDSIVKDILIFENVYADAGPDRILPFGQVYQMQGNSTATGVNYQWS
                     PSFGLSDPFIRHPEVRADRDIEYILEVNNEVGCFDRDTVKFRYMKGPDIYVASAFTPN
                     NDGLNDVLNFFPVGMENAKLNIYNRWGRLVFSTADLSQGWNGKVKDLLQDTGVYAWIV
                     KATDYKGQSIIKKGTVLLIR"
     gene            complement(23186..24799)
                     /locus_tag="PLO70_06655"
     CDS             complement(23186..24799)
                     /locus_tag="PLO70_06655"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ74177.1"
                     /translation="MKIRIVSTILIAGALSLSSCQKKVLDLVPFTAQSDESAFANPDA
                     VQLSVYGVYDACQSGFYAGGAVRGYPFGAASIEQGDMRGEDMLNQAAFFQITYEATYN
                     NASPNNGFMFQTIYSMANKANLVIDGVKGAGSKGIITSAVALQYEAECRFLRAMGHHE
                     LVLNFSRPYADGAGAQMGIIYRDFAIKDAATADQARAIKRGTIKVSDNYASILADLDF
                     AEANLPATPVVNTAGTAIKTYRASKAAAIALKMRVKMHKGDWPGVITEGNKLVPAVAP
                     FVSPIGGWTLNAAVDGAFANNTTNESIFSIKNDATDNPQVNGALPQMLGDPALGARGL
                     VRVSPVLWSDPQWLCSDLRRSALYRTSGSPVNAIYTVKYRDYANSSDAAPMIRYAEVL
                     LMLAEAEARQAAGTTVSTRGVALLNAVRNRAVTTPADQFTVASFADKNALISAILKER
                     RIEFLAEGKRFGDIHRLALDPDFAPIAGGGIPSKVGSGGATAAMFNCAGASFTRSVPA
                     LAYSNFRFLWPIPIEETQQNVNYDQNPGY"
     gene            complement(24823..>25371)
                     /locus_tag="PLO70_06660"
     CDS             complement(24823..>25371)
                     /locus_tag="PLO70_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74178.1"
                     /translation="AGTLAGGDRQILGSALPKYFGGITNTVRYRQFTMDMLWRFAGGN
                     KIMNITKQESLMNQGFMNNGTYILNRWQKPGDVTDVPRLWYNRDNFTNLNQQASTRFI
                     EDGDFFKLDNLSFSYDVAPALLSRMFKSSVKSFRFFVQGQNLFVITDYTGIDPDNISE
                     TGLDYNSIPPARTISFGINIGF"
     assembly_gap    25372..25471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25472..28097)
                     /locus_tag="PLO70_06665"
     CDS             complement(<25472..28097)
                     /locus_tag="PLO70_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ74179.1"
                     /translation="MRKLYLLLLGVVFFATQAMAQRTITGMVTDEKGNPVANASVMVR
                     GTTVGTTTKADGTFTLSVPANARAIIFSSVDMITQEVSIGSQTTISVSLKGDDKIMQE
                     VVVVGYGTQQRRQATAAISKINPENIAPLVSTSIDKQLGGRSAGVLVTNPSGLVNEPP
                     RIRIRGVNSINGARGPLIVLDGIPTFSGGFAGFTNDNVLANINPADIESIDVLKDGSA
                     TAIYGSQASNGVIMITTKKGKSGRQNFNYSAVFGFAKPLNRFDLLNAQQFVTIVNAKL
                     SAAGLANGAFMNSENTNTDWQDVVFRPTSQASTHNLSLDGGNDRTTYFLSFNYRYQEG
                     LVKTNTSENYNIRANIEHKVNKWLKVMNYITLSRAFDKDQNNGGNALSGSVAAALRAL
                     PNVRLFNPSLPQFDFYNITPDGGALGSDANTRLIENNYVNIGYVLAKNKFRSKKHRVV
                     NNFGIEIKPTSWLTNTIRIGIDYITLDEFFRQDAKHGDGRSVLGRVSNQAANTMRWTA
                     QEYINASKTFGKHGFNLTAGIEAQKQENNSFSASGTNVADAFFQQQNVISGSYVTQLS
                     AGSYSQGPGLFSYFGRINYDYGGKYFLQASFRRDGLSRFAKENRFGSFPGGSIGWRVS
                     QEDFWSTSGLDKVISDFKVRASWAKVGNSAIAGGLFPYLNLYGLAPYGAISGIAASQS
                     GNAALQWETNEKIDYGFDISLWKDRVTFVFDYFKNKNNNLVLAAPQPPSFGVPGNQIF
                     QNIGSMENKGIELALNIAVVKRKDFSIDLGFNYTNQSNKVLSLYLNQDVIQSNGTGNY
                     NILRVGQPIGALYGYQFVGVNSSNGNPVYRKADGSLIMGNITNSSYFAIADLNSSTIG
                     AAGTLAGGDRQILGSALPK"
BASE COUNT         8633 a         5306 c         6186 g         8000 t
ORIGIN      
        1 ccaatcaggg tactgtcaat tggttcagcc ggattttttt ccagcggaac cattgattcg
       61 tatggacagg catacagaat aatggaaagt ggtaataacc acaacagctt ttgcattact
      121 taagaatgga aaaagatacg aatattgtct tatatcgctt ttgtctaaca accagtatta
      181 attaggatgc ttatttttgc gcatgcggct tagaataatt ttcttattta ttcaattgat
      241 tttttgtgag ttgtatttaa aagcgcagtc accaacttat cccaaagagt atttccgtaa
      301 cccgatgggg atacctatcg atttatcggc caattttggt gaattgaggc caagccactg
      361 gcatatggga ctggatataa gaactaatca aaaagaaaac ctcttagttt atgctgctgc
      421 acagggctat attgccaaaa tcggtatccg gtcgcagagt tttggacgtt ttattgttat
      481 taatcaccct aatggactat caactctata tggacattta aatgacttct atcctgaact
      541 ggagaaatat gtaacagcac agcaatataa gcaggagtcc tgggctgtgg aattggatct
      601 ttccaaagat aagtttccgg tgagtaaggg tcagtttatt gcttatagtg gtaatactgg
      661 tggttcgcaa gggccacacc tgcattttga gatttttgat acaaaatcta caaaacgact
      721 gaatccgcta ttatttgact ttggtatcag ggataatgtt ccgcctgcta tagtgaaatt
      781 agcggtgtac gacagaggca ggagtgtcta tgaacagtca ccgctattga ttgcattaaa
      841 aaacactgac agtggttata tcattccaaa gtttccggta atgaaaacgg gatttagcaa
      901 acttagcttt gccatccagg cttatgacag ggtatccggc tctaccaatc ccaatggaat
      961 ctatgcagca aaactttttg tagacgaaca gccgcaaatt gcatttgttt tggatagtct
     1021 cgattataat gaaacagtct atttgaatgc tcatgtggat tacaaacata aacacaatgg
     1081 cggcgcttgg ctgcaacatt tgtcgaaatt gcccggtgat gcaggggcgg cctataaaaa
     1141 attcaatggt gatggtgtta tctctgttac tgacacagca atacatgata taaggataga
     1201 ggtaaaagac gctaaaggaa atatgtcaaa attgaatttc cggcttcagt acattgatag
     1261 cctggcaaag ccttccataa accaggaggg ttctatatac tttgccccga atagtaaaac
     1321 tgtgctggca aaacctgatt tcgaaattca tttacccgga ggcagtttat acgatactat
     1381 gaaagcttat tatttccgta ataatactgc agtagcaaaa aatgctatct cagctcttca
     1441 ccaggtaaat gatgcatctg tacctgttca caacaacttt actgtcttta ttaaactcta
     1501 taagcctgta ttggctgaat ggagcgataa attgttaata cagcgcagtt accggaacag
     1561 caatacagtt cgtaaagcgg ttttaaaagg agagtggata acaacatcgt ttgatgattt
     1621 tggttcattc caggcatttg ttgatacgat accgccaagg ataagtgatt tgaaaaatga
     1681 cgatacaatt aacttgagtg cctcttcccg aatcgttttt acccccactg ataatttcgg
     1741 ggtgataaca aatttcaggg cagagttgaa tggacaatgg ctgcggttta ccaatgataa
     1801 aaccaggaac tggatataca aatttgatga acgttgtccc tatggggtgc atgaactgaa
     1861 agtaacagca ggggatttgg ccggaaatat aacaatgaag acctggtggt tcaaaagagg
     1921 gccatatact ccgccaccaa aaaagaaagt gatgaagaaa aagagcagta caaaaaggaa
     1981 aactacagcc ctcaaaaaga agtaaaagga caaattaaat tatgataaaa ctgaaagaag
     2041 gtgataaagc cccggtattt acaggcaatg accaggatgg aaggaaagtc tcgctggctg
     2101 attttaaagg gaaaaaagta gtgctttatt tttatccgca ggatatgaca cctacctgca
     2161 cagtgcaggc ctgcaacctt cgggataatt tttccatgct taagaaaaaa ggttttgaag
     2221 taattggcgt aagtccggat gatgttgcaa agcataaaaa atttgaacag cggaataaat
     2281 tgccattcac acttattgcg gatatatcac ataaaatact ggataaatat ggtgtgtggg
     2341 atcagaaaca actttttggc cataaatata tgggtgtatt gcgcaccact tttgttattg
     2401 acgaaaaagg aattatctgt aagatatttt taaagccaaa gagtaaatca catgctgaag
     2461 aaattatgac tgccgtaaaa taattctata cacttgctct gaaaccgttt tattgattca
     2521 actggcatgg gcaaccctaa taggtttata aacgattctt atcggattat aaacccatga
     2581 tatgaagcag ttattcaccc ttgcagtatt ttttatttcc tttattgtag ccgcccagga
     2641 cagcgccata aaaattactg cgggtaaacc tgtaagaata ttcaattcca ctaaaacgat
     2701 caatgctcgt acaacagaag tgaccggtaa gggcaaaatg gattttaatg tcacccacaa
     2761 ctttggtgat attgccggtg ataatggcgg gataaaacgg tttttcgggc ttgataatgc
     2821 tgcagatata cgtattggtt ttcatatcgg gatgggacat aaaactgacc tcgttctggc
     2881 aagggctaaa ggagcaagcc aggtacaaag cctttgggaa attggtgtga aacaccaatt
     2941 attgatacaa aagcaggacg atccaacaca tcctttatcc ctcgccatat atgccaatgc
     3001 tgtaatctct tcccaaaagc gaagtccgtt tgagaaccag gataacagtt ttaccggttt
     3061 agctgaccgt accagcaatg tggtgcaact gataattgcc aggaagatca ataagattag
     3121 tttacagctg aatcctacat ttatgacacg ggggtatgct gtttcctacg atcagaaaag
     3181 catgtttgca ctcggtggcg caataaagct tcctttggtg gccaaccgcc ttaacctggt
     3241 agtagattat tttcatcctt tccgtaagca ggctacaaaa gattccttcg ctgtaaatga
     3301 gaatatcaga ttctttgatc cgttgggagt gggatttgag atacttacat ccgggcacat
     3361 tttccggctc aatttcacta acgcaacaga aatagtagag aaccgtttca ttccccgaac
     3421 tatcacctcc tggggaaaag gtcaatttcg ctgggggttt actatttcaa ggaactttac
     3481 attgtggagg tgagtacata agtgatgaac agtgaatagt aaacgggaag taaccagcac
     3541 atgcagttat agaagctttt ttattgcaac ggtaaagaca agtttaaagt catagaaata
     3601 tgcaggtaca gataaaagaa aaatcatggg tggcttattt ggcagcggca aaattgaagg
     3661 ccgataaagt agccattgtg tttggttcca ccatacatct gcacaacaca agcagagagg
     3721 agtttttaaa ggataaggat tggatatgtc atgaactgaa acatgtggag caatacctgc
     3781 agcatgggta tatagggttt atcctcaaat atctttttga atggatcaag aagggttatt
     3841 ataataataa gttcgaagtg gaggcaaggc agagtgaaaa tgaaatttca ttacttcaaa
     3901 aagccaacat tacttaaatc atccattcca actctccaaa gtcaaattcc tcttctatcg
     3961 catgctgcac tactaatttt ccggcaggag ttactttctt tataaccgct tcaaacaccc
     4021 tgttttcttt cctcagtttt actttttcct cctttttgta caggttggaa atataattgg
     4081 aatatatact atgaaagcct tctttaatta acagatgata atacttctca atgtaagtac
     4141 agatatcttt agccagctct gccggctcaa aaattttccc ggttatctgt cttaatgaaa
     4201 cagggttggg taaatcgggg gggaaaactg tttgattaat attgattccg atgccaatta
     4261 tagaccagtc ccaatttcct gcgcctgatg aagaacttcc gacaatattc tcgattaaaa
     4321 taccccctgc ctttctgtcc tgccagtaga ggtcattggg ccattttatt ttggtgtcgt
     4381 ctccggtata gttcataaaa aattcatgta ccgccactgc cacacaggca cttaggtgaa
     4441 attgctgtgt aagccttaat gatgcaggtt ttatcactac actaagggca atattagcct
     4501 ctttttcgga ggtccagacc tttccccgtt gcccttttcc tgctagctgt tcatgcgcaa
     4561 aaatggtcat tccatgttgt gccaaaccag catgaatttg agcccgggca tagttgttgg
     4621 tactgtcaac cgattgcagt tcaatgaatg gcaaaccgac aggagttgtg gagaagggtt
     4681 gtggcaatta attgctattt ttgacgaagt aaacttaaac taaaaaacaa acactctcaa
     4741 tatttggaac aattggcaat actgaccaac cgtaaaaaga aaagcttgtc cgaaggacag
     4801 ccgggtgcaa aaaggttaac gagaaactca aaaatcatca agaccatcat tgctgctata
     4861 caggaaaaaa agggggaaaa cattgtttct cttgacctcc gaaaaattaa tgaagcagta
     4921 gctgatttct ttattgtttg cgaggcaggc aatcagccgc agataagggc tattgcagat
     4981 aatgtgcaat ataaagtaaa ggagaagtgt gaagaaaatc ctttccacca tgaaggatat
     5041 aataacctcc actgggtgct gatagattat gtaaatgtgg tggtgcatgt attgctacct
     5101 gagaacagga agttttacag gttggaggag atgtggagtg atgcggtatc acaggagcac
     5161 aatgagtaat gttaaaatag ctgcccgcct caactttttc aaggaataat tattaattag
     5221 caggaatacc ttatagaata atatgtcaca agaatcaaat aaaagaaacg aaacgaagcc
     5281 tccaagattt aataaccgcc cgggtggagg agaagatccc caggctccca aaaaaggccc
     5341 ccgcttcagt atttactggg tctacgccat catttttgcc gtgctgatag gttttcagct
     5401 gttcggcccg cttacaccca atatggctaa aaccaatgaa ctggagttga agaatatgat
     5461 tgccaaaggg gaagtcgaaa aatatatgat catagataac cgtaaggtgg tgagggtgta
     5521 cctgacagct gcaggtatta agagtaatga ggtaaagctg aaagaagtat ctggcaaaat
     5581 accagagaaa ggtcctcata tgtcatttaa gattaccagc ggggatgctt ttaaaaagga
     5641 aatgagcgat tattacaagg aaaatccaac cgtacaacag gtagtttttg atgttgataa
     5701 tgaaagggat tggtttggtg gcatactgca attcttgtta ccagttctat tgtttgtagg
     5761 tatctggata ttgctaatga gaaaaatggg tggcggcgca ggtggcggcg ggggtcccgg
     5821 tggtattttc agcattggta agtcaaaagc tactttattt gataaaggca caaaagtaaa
     5881 tatcacattt gctgatgtgg ctggtttgga tgaggcgaaa gtagaagtga tggagatcgt
     5941 agactttctt aagaacccga aaaaatatac caacctcggg ggtaaaatac cgaaaggtgc
     6001 attattgatt ggtcctcccg gaaccggtaa aacattactg gcaaaagcgg tggctggtga
     6061 agcacaagtt ccttttttca gtttaagtgg ttccgatttt gttgagatgt ttgtaggggt
     6121 gggagccagc cgtgtacgag atctgttcaa acaagcaaga gaaaaagctc cctgtatcat
     6181 atttatagat gagatcgatg ccattggccg tgccaggggt aagaatgcaa tgatgagtaa
     6241 tgatgaaaga gaaagcaccc ttaaccagtt gctggtagaa atggatggtt ttggtacgga
     6301 tgtaggtatc attgttttgg cagcgacaaa caggcctgat gtattggata cagctttatt
     6361 gcgtcccggt cgtttcgaca ggcagataac aattgacaag ccggatcttg tgggtcgtga
     6421 agcgatcttc aaagtacacc tgaaaccgat caagatttcc aagacattgg atatacataa
     6481 actggcagaa caaactccgg gttttgcagg ggctgatatt gccaatgtat gtaatgaagc
     6541 tgcattgatt gccgcacgaa aaaataagga ggcggtggac atgtctgact tccaggatgc
     6601 agtggacagg gtgatcggcg ggttggaaaa aaagaacaag atcatctcac cggaagaaaa
     6661 aaagatcatt gcctatcacg aagccggcca tgctatttgc ggttggtacc ttgaacatgc
     6721 atatccttta ttgaaagtaa cgattgtacc aagaggtaca gcagctttgg gctatgcaca
     6781 atacactcct aaggaacaat acttgtataa tatagatcag ttaacagatc agatctgtat
     6841 gactttgggt ggccgggcca gtgaagaaat tttctttaat aaaatttcta ccggggcaca
     6901 gaatgatctt cagcagatta caaggattgc ttattctatg gttacagtct atggtatgaa
     6961 cgataaagtg ggaaatgtta gcttttatga tccgcaacag gaaacatctt ttactaaacc
     7021 ttattccgac gagaccgcta agctgatcga taatgaagtt cgcaaactaa tcgatgacgc
     7081 ttatgaaaaa acaaaggatc tgcttactgt gaagaaggca gatgttgaaa aacttgccaa
     7141 tgcgctgctg gaaagagaag ttttattcca gagtgatgtg gaggcattga ttggtaaacg
     7201 tccatttgaa gaaaaaaaac cactcgtaga tgaagtgaca gtggaagaac ctgttgaaga
     7261 agcaatacca gtggcagagc caaagaaaga agaagaaaaa tcgacagatg agcaggccgg
     7321 aaatatcggt ttagcaccgt ccatttaatt cgataatatg aacgtttctc cttcaaaaga
     7381 aaatatattg aagaaaatac gcaaggcgtt gagtcattca acgcctttgc cttttccgca
     7441 aagcgaagga actaactctg tattcgaacc gcttcaacag gaagctgagg ttgaatttgc
     7501 agagcagttt actaaactac agggaaaatt tatttattgt atcaaccggc aggaactggc
     7561 ttttcagttg ggaagcctgg ttaaaaaaca ggattggcaa aaagtgtatt gcgtagagga
     7621 taaactcatt gaaccagtag ctttacaact gtctgacaga ttgataaaaa ccggtctaga
     7681 aaattgtgat gtttctatca ctggctgtga atacctggtt gccagaacag gttcaattgt
     7741 aatgagtacc gcacaaagta gcggccgcag caccagtgtt tatgcaccga tacatatctg
     7801 tattgctttt accaaccaac tggtttacga tctaaaagat gccctgcagg ctgcaaaaga
     7861 taaatatggt aataaccttc cttccttgat cacttttgca acaggcccca gccgcacggc
     7921 agatattgaa aaaacattgg tagtgggtgt tcacggacct aaagaagtgt atttattcct
     7981 tgttgagtca taaatcaccg ggctttagta cttttatgtt atgacaagtt caggcattta
     8041 caaactattt gtatacggct cattacgcag cggctttcac agccatgtgt atgagtatat
     8101 cagcagatac tttaacctgg ttggagatgc aaaagtaaaa ggcaaattgt ttgatatggg
     8161 aagttatcct gcagggattc ctgcagagga agaggtgttc atagtaggtg aattgtacca
     8221 gattaagcat gaaaatgaat tttcctgggc ttttggccag ttagatgact atgaaggagt
     8281 aaatgttgaa gaagatgaaa tgcaactgta tcgccgtgaa ataacagatg taatttgtaa
     8341 cggccaggtt acaaaagcct ggatatactg gtttaatggt gaagtaagcg gcaggcctgt
     8401 aattgcttcc ggggatatga tagagtatct caatcaaaag aaatcttcat gaaattaccg
     8461 gcttctatac tgttattttt aagttgtgtt tttcttttat catcctgtga gttcaattgc
     8521 tccgtaggac aaaaagataa aaatagcagc gctgtctcca gagaagatga accccgtatt
     8581 tataataata tcaatctgaa ggcaacagga ttaaaagtag aaaaagccta tctcattttt
     8641 gaaaatggtg atggggtacc cgagaataat gcagtagatt ttacaagccc tgtaaaaatg
     8701 atcgtgaaaa tagatacagg ctgggtagta aaaaacgata aagtgatgat aggagcgtca
     8761 gaaaaaatta tatccgaaga tggaactgtc ttactggata agcctgattt gtttgctgat
     8821 gattctggtg tatcgccaca agatgcaaaa gctatatcca tatgggctca aataagatta
     8881 agagaaggtt cacctcctgc atcattcact atatcattcc ggatttggga taaaaacggg
     8941 aaggggacaa tagagggtaa ttacaaatta tattctaaat aattcaattg ctccggcagt
     9001 atactgaaaa ataaacgtgg ctaaaaaaat ctatttcctt tctgattttc atcttggcgc
     9061 tcccgatcat gccagcagtc tcgaaagaga gaagatcatt gtacaatttc ttgatgagat
     9121 taaacaggaa gcagcagaaa tctttttggt cggcgatatg ttcgatttct ggtatgaata
     9181 ccggaaagtt gttcctaaag gttttgtccg gttattagga aagctgggcg aattggctga
     9241 tgccgggata tcgatgcatt tttttgtggg taaccacgat atgtggatgc gggattattt
     9301 tcagcaggag tttaatatgc cggtttattt tcaacccaaa gaatttgaaa gaagcggaaa
     9361 gaagttcttg atcgggcatg gcgatggcct gggacccggc gatcatgggt ataaaagact
     9421 gaaaaaagta tttcgcaacc cggtgagtaa atggttgttt ggcattttcc ctcctctctt
     9481 gggcatgggg ttagccaatt acctcagccg ccgcagcagg gcacagacgg gagcttctga
     9541 agagatattc ctgggtgagg acaaggaatg gctgatcatt tactgtaaag aagtgctggt
     9601 acagcaaaag atcaattttt ttgtattcgg acatagacac ctggccattg agtatcgtct
     9661 gaatgaagag agtaaatata ttaacctggg tgactggatc cgttattaca cctatgctgt
     9721 atttgatggc tccaccttgg aactaaaatc ttacaaaggg aatgatgaca agatcatcag
     9781 gaaataaatg aaaaaatttt cagccatatt gtttgtttcc tttttgttgt ctgctaccgg
     9841 gttttcacaa gccgatacaa ctttccagtt acttaaaatc ctgaaaggag atatcgttga
     9901 ctttaccgtt gacaacctgg acaatattta tgtactcaac agccggaacc aggttaaaaa
     9961 atatacagct aacggagact cagttgcgat ttataatgac ataagaaagt ttggcagagc
    10021 tacactgatc gatgtttcta accctctaaa agtgttactt tattaccgtg actttgcaac
    10081 agttgtcatg ctggatcgtt tcctgaatgc agtcaatact gttgatatcc ggaagcagaa
    10141 tatttttcag gcaaaagtta ttgcccaatc atatgataat aaaatctggg tatatgacga
    10201 actggagagt aaactcaaaa aaatagatga agatgggaag ttattacagg aaacacctga
    10261 tttcaggctt ttattaggct atgccccttc accatataaa atatttgatg agaataagta
    10321 tgtgtacctg tatgattctt tgaaaggagt gtatgtattt gattatttcg gatcgttgaa
    10381 aaacaatatt atgattcaac gctggcaaaa cctgaaagtg accggaaagt ttatttttgg
    10441 ttcaaagcag gatacacttt tccgctacga catcaatact tttatgtacg atgaatggaa
    10501 aatacctgaa caaattaccg gtgccaggtc attcaatttt agtgcaagtc gcttgtacgc
    10561 attgcggaaa gaatgtgatg ggaagctgga ctgcatttct atttatgcca tccgctgata
    10621 ttttcttctg cgaattcaat aattttatag ttcaaatttt cttatttcat gaatgacttt
    10681 ctggaacagg tatggtggga taaccccgta aggaattacc ttatagcagg cggcatcata
    10741 ttatttgtat ggttattcat gcggtatatt tcccgctatt tagccggctt actctttcgt
    10801 attgtacata ggatctggaa taatgtagat aaacaatcat ttaccagcct ggtgatgaag
    10861 ccccttggtt tttttctgct gatactggtt tctattgctg ctttatataa actgaatttc
    10921 ccggaaataa ttaatgtgac aatctacaaa tacacggcaa aagagattgt acattgtgtt
    10981 ggtacaattg tcctgattgt ttcttttatc gggttattac tcaggatcat tgatttcata
    11041 gcagtattgc ttgaaaaaaa ggccaatctt acgcctgatc agtcagataa ccagttgatc
    11101 gttttcttta aagatttttt taaggttatt cttgtaatca tcggcataat gatggtgctg
    11161 aaatatgcat ttgacttgaa tatcggaggc ttgcttacag ggcttagtat tgtgggagct
    11221 gctatagcat tagccttgcg ggaaagcctg gaaaacctga tcgcttcctt tattatcttc
    11281 tttgataaac cttttattat gggtgacctg gtaaaagtga ataatataac aggctctgtt
    11341 gaacggatag gcttgcggag tacccggata cggaccgatc agaaaaccta tgtaactgta
    11401 cctaataagc aaatggtaga tagcatactg gataaccttt ccctgcgtac tcaaagaagg
    11461 ggtgatctga aactggaaat tggattgcaa acggcacccg cagctattga acaattatta
    11521 gctggtatca atacaattgt tggacgaaag gaggtagaga atgcaaatgt gctattgtca
    11581 gatataacaa gtaatgctat agttatcctg tcagattatt ataccgcacc tattaccata
    11641 caggagttta atcaggtaaa acaggaaata aacctgcaaa gtttgcagtt gctcggggaa
    11701 ttaaagattg aaattgcagg tagcagtact gatgtaagaa tcagcaaata ggctattgtt
    11761 tgtttaggga gtttattatt tctcctaatt ctttatcaaa cattccatat ttaccatatt
    11821 ctaccacacg accggcttct tttccattct ttaaaacaat gatggtggga acattaatta
    11881 cattcaacgc ttcagtaaga tggctcagtg tttttttatt tctgtctaca ccaatcaggg
    11941 aaatacggtc ttgtgaaaag ccagctgcat ccgttaaagc atagaatttt ggtataatga
    12001 aatgggagtc ttcgcaccag gtacccatga aaaccagtaa ttggattgag tcagcctgtt
    12061 gctttaaagc tgcaattgca gctgtattgg ggctgaaggc tttgtaatta gtggcaaacc
    12121 acttaaatga agtgtcactc tccagcaccg accgggaaat aatacctttc aggctttttt
    12181 cattgggtct ttctaccaat gtttcaaact ggttttgggc aaaaacaccg gtagtaaaaa
    12241 taaatgcaaa aattacgggt ataaactttc tcatgtttcc aggtaaatag tcacaaatgt
    12301 aaaaagagaa caggcaaaag atgaaagaag aggtgttaaa gttgcaaatg gtccttagct
    12361 gttcgttcaa acattacaat aaccaaaaat taagaaaatt agtccgggag atcacaggtt
    12421 tgctttcaat tccatcagaa agccctttat tttttgcaat gattgtatca tcgcaaattt
    12481 ttcttctgct cttttatttc ctttcaggta gtctttggcg ccctcgatgg tgaatttgcg
    12541 acgccgaagc agatcgtaga tcatcaccag gttcttgata tcaacagggc ggaaaaaacg
    12601 gtcgcctttt ctgttcttgc ggggttgaag aatatcaaac tcagtttccc agtaccgtat
    12661 caatgattga ttttccctga acatcgccgc tacctcaccg atggagtaat actgcttttt
    12721 aaaaagaatg tcatcttccg ggacatgtac caggtcagca tttacagcaa tgtctttgag
    12781 tgaacggcgg cctcttttac cagcagtagc tttcttttct tctaatacga tcgtactcag
    12841 gacaggtatt tccggttcag ctaattcaat ggtgggtact tcctctttta cggttgaagt
    12901 aatgacaacc ggtttttgct ctgtttcttc aacagcgcca aaatcaaaag ttatttgtgt
    12961 aagccccttt gtcataacaa taatgtaaag ataacaaagc cggtaccgga taattgttat
    13021 aaaagtgtag tgtggaaaaa aatagcaaac tgaggatcag gctgatttta tgatctttaa
    13081 tacgtagttc tttaatccag gctttgatta cttgctgccg cttttttcag aataagatca
    13141 aattgctctg gcgtcagatc attataaaaa aaatagatcg ggtcaatttt ctgtccattt
    13201 ttatgtactt cataatggca atgtggtcct gttgatttac cggtacttcc cacccagcca
    13261 ataacttcac ctctttttac ggcttgcccg tttcttgctt ttactcttac catatggccg
    13321 tataacgttt catacccgta tccatgattg atgataacat gattaccata tccatttcca
    13381 ctattaccgg cagtgcttac tgtgccatca gccgttgcat aaatgggggt accctgtgga
    13441 gcagaaaaat caagcccggc atgaaacttt gtagttttgt aaaccgggtc aatccggtaa
    13501 ccatagcccg aagcaatacg gttcagatct ttattactta ccggttgtat ggcaggcgta
    13561 cgggcaagga gtttttcttt atttttcacc aggtcatcta cctcatcata agaatttttc
    13621 tgtgcattga tcctgcttac caggttgttc agcgtattat tgatggagga taccaactgg
    13681 tgatccctta gcttttcgac cttggcaatc tccagtttga tttccatttc cttcgccctt
    13741 gcgctgtctg gtatgggatt ggcttcaaaa atggccctgt acacatcatt atctcttttt
    13801 tccagttctt tcatttgttg ctgaacagac tttaattcat ccagcaggtc gtagtaattg
    13861 tcttttagat ttttattctg ttgctgcagt atcctttctt taggggagcc aatgaattgg
    13921 aaagcggcat aagaaataag gcctgcggta acaagggcgg cagctaaaaa accaaagacc
    13981 cggagcagtt tgacccgcat gggtgtttcc agtttttcat accggagcgt attggtatta
    14041 tagtaatatt tgattttctt cattgaggca agccttaagc ccggttcagc gaaataatgc
    14101 cacctgaata ttttaccttt gcccggcttt tacggggctg acaaatatag cccgttaaac
    14161 tgcaaaaatc aacccaatca cagcagcaat tatgatgacc agcgcagata tacgacaggc
    14221 tttcctggat tttttccgtt ccaaagggca tgaaattgtc ccttcggccc caattgtggt
    14281 aaaaaatgac cctaccctga tgttcactaa tgcggggatg aaccagttca aagactattt
    14341 tttaggaaat aagccagccc cctttaaaag agtggccgat actcagaaat gtcttcgggt
    14401 aagtggcaag cacaatgacc tggaagaagt gggagtagat acttaccacc acaccatgtt
    14461 tgaaatgctg ggaaactgga gttttggcga ttattttaaa gatgaagcaa tagagtggag
    14521 ctgggaatta ttgacagttg tatataatat tcccaaagac cgcttatatg ctacggtttt
    14581 tgaaggggat gtaaaagagg ggataccggc ttcatctata gcattggcca agtggaaaag
    14641 ccttttaccg gatgagcata tcgtgtttgg taacaagaag gataattttt gggaaatggg
    14701 tgaaaccggt ccctgtggcc catgcagtga gatacatgtg gactgcagac ccgatgaaga
    14761 aagaaagaaa ataccgggtc atacactggt aaataaagat catccgcagg taatcgagat
    14821 ttggaataat gtatttatac agttcaatcg gaaaaaagac ggtagcctgg aaccattgcc
    14881 tgcaacccat gtggatacgg gaatgggatt agaacggtta gtaagagttt tgcaaggcaa
    14941 gacatcaaat tatgatactg atatttttac cggaacaatt gctgaaacag aaaagataac
    15001 cgggaaaaaa tacgatttca gcgatagcaa gacagctata gctttccggg tgattgcaga
    15061 tcatatccgt gcaatatcat ttacaattgc tgatgggcaa ttaccctcta atacgggtgc
    15121 tggttatgta atccgccgga tattgagaag ggccgtgcgg tattattatt cttatctcga
    15181 ttataaacaa cccctgctta cccagttagt gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnctgaag tggaagtagt ggataccaaa aaagataatg accttatcat
    15361 ccattttaca gaaagtattc ccgctgactt atctggtgaa gtgaccgcaa aagtggatat
    15421 taataaaagg aaaaacacgg cattacatca ctccgctact cacttattgc atgctgcatt
    15481 aagaaaagtg ttaggtacac atgtagcaca aaaaggttca ttagtaaatg aggaacatct
    15541 ccgtttcgac ttttctcatt ttgcaaaagt tacggaggag gaaattgctg caatagaagc
    15601 attggtaaat gaaaagataa gagagaatat acctgttatc atcaaatcaa tgccaaaaga
    15661 ggaggcgatg aaactgggcg ccatggcatt atttggcgaa aagtatggtg atgtggtgag
    15721 agtagttgtg attgacccga actattccgt agaattatgt ggcggcactc atgtgggtgc
    15781 tacaggtgaa ttaggtttgt tcaagttgac cagcgaatct gctgttgcag cgggtgttcg
    15841 cagggtagaa gctgtttgcg gtaaacaagc agaagaatat gtaaatgaac aattgaatct
    15901 tatcaacagt cttcgggaaa ccctgaaaaa tccaaaggat attcaaaaag ccatcgggaa
    15961 tttgctggga gaaaatgccg ggctgaaaaa acatatggag gctatggaag cccgccaatt
    16021 ggttggtatc aggaatgaac tcctgcaaaa agatgagatc attaacggta ttaattttat
    16081 aggagatata attgaagtaa gtaatgctga tgcattgaaa aaattatgtt tcgatttgaa
    16141 gaatcatctc cgggaccatg tagcggtgct ttgcgccaat attggaggta agccttttgt
    16201 agctgttggc atttccgaaa cagtggctgg tgcaaaaaac atggatgccg gaaagatcat
    16261 taaagaacat attgctcccc tgataaaagg cggcggcggc ggtcagaaaa cattagcaac
    16321 agccggcggg caggaggcag gaaaattgaa ggaagtaatt gaggcagtaa aaaagctttt
    16381 atagattttc agcggcgaaa agggaagtct tcatcatccc tgtacttctt ttgttgtttt
    16441 tccagctctc tgttttcttt atagattttc agggccaata taattacgcc aatacatata
    16501 aggattattg taacagagat aaaatattcc ataaatacta ttacttaaca aaaggggaac
    16561 atcaaaattc cgcttttccg ctgaaattac aataccactt taaggttagt aaaaatactg
    16621 ttagcgtctg actatgaacg ttatattaga aactgatcgc ttactgctcc gcacttttac
    16681 aacggatgat gcccggctca tttacgaatt aaatgccgac ccggatgtta cactttatac
    16741 tggagatcct gtcagtgatc tgtctcatgc tcaacaggta ttggatcagg tcattcttcc
    16801 tcaatacgct ttatataacc atggtcgctg ggctgtacat gttaagccca ctcttgaatt
    16861 cattggctgg tgtggattga aaaacaggcc cgaacgtaat gaaatcgacc tgggataccg
    16921 gtatctgaaa tcggcctggg gaaaagggta cgctacagaa gctgcctttg cctgcataaa
    16981 gtatggtttt gaaaaactgg gtttgtatcg gattgtggga agggctatgc ccggtaatgt
    17041 ggcgtccctc cgggtactgg aaaaatgcca gatgacctat atcggtgagg aaatagtcga
    17101 cggccacccg gctaaaacgt atgagagcat caatccgctt atcctttaac tattccccgg
    17161 cctctttgaa tacagcatgg tcaggtagat attgccgttc gaaaactgta agggttttaa
    17221 catttccgaa acatttcgta tttcagaaaa tctctttata tttgatatac gaagaataac
    17281 gtagtttgtt taataaatta aatatcattt tatgcgaatg acgtggatac aatcatctgt
    17341 cctggctgtt acaattgctt taatggcacc tgtagtagta ctggctcaaa aagatgaaaa
    17401 agacaaagtg aaaaaagatg tacaacaaat tatcatcacc cgtagtggcg aaaaggatga
    17461 taaagttgtg gttgaaatca atggtgataa agttaccgtt aatggtaagc ctatcgatga
    17521 gtttaaagat aaggatgtaa aagtccgggt taacaggctg agggattctg aggcttttgc
    17581 ttatccccgt tctccaaggg gtggtacctg gaatttcaat gaggatgata atgtacgctt
    17641 ttttaatact gatgcaaaca gggcaatgct gggtgtgaca acagagaagg acgaaaaagg
    17701 tgcaaaagtg aatgacatca caaaagaaag cgccgcagag aaaattggtt taaaagaagg
    17761 tgatatcatt acaaaaattg atgaccagaa aattgagagc ccggatgacc tgtctgaagc
    17821 tattacaaaa cataaacccg gtgaaaaggt aacggtaacc tggtggaggg acaataagga
    17881 acaaaaggct acagcagaac tgacaaaatg gaagggagtt aatgtttttg gatcaacaat
    17941 gccgggccag caatttaaga tggatatggg agatatgaat tttgaaaata tcctgccgcg
    18001 tctccaggga ttacaggggc tacgggagcc atttgcacct ggccgtccgg gagccgggcc
    18061 aaagctcggg ttatcagttc aggatactga tgacggaaaa ggagtgaagg tcattgaagt
    18121 ggatgatgaa agtaatgctg ccaaagcagg gatcaaagaa aatgatgtaa tcactgatgt
    18181 ggatggtaaa gcaattaaca gtacagatga tatggtaaaa gtgatcaggg aaagcaaaga
    18241 gaaagtttca gtaatgatca aattacagcg tagcggtaaa acacagaata tagaagtaaa
    18301 aatgccccga aaaataaaaa ctgcggatct gtaaacctcg tttaatattg gtgtgtggta
    18361 actaaagcct cgtcctgcct ttggcggatc ggggctttaa cttttttgcc ggtgcctata
    18421 atcatcaaat cacccaacga caattttcag ctttaactat ttaggttact ctactgcctt
    18481 aaatgggcta tttttgccat cctgaaaatg gagaatacca gtttatatga aatagttgtg
    18541 gggctggagg tgcatgcaca gttacttacg aaaagcaaat tgttttgcgg cgatagtatt
    18601 gcttatggag cagaaccaaa tacacatgtg agcccggtaa cacttggtca ccccggtacc
    18661 ttaccgaaaa tgaacatcaa ggccattgaa tttgctatca aaatgggatt ggcctgtcaa
    18721 agtgaaattg aaaagcataa ttattttgcc cggaaaaatt atttctatcc tgacctgcca
    18781 aaagggtacc aggtttctca gcatactaca ccgatatgta aaggcgggtt tgtaaatatc
    18841 aaaacgcagg agggcgggaa acaaatacgg ctgaatcgca ttcacataga agaagatgca
    18901 ggtaaaagcc tgcatgatgt ggacgaggcc aacacctgta ttgattataa cagggccggc
    18961 acccccttga ttgaaatcgt tacggaaccc gatctgcgga gtgctgatga ggcatttgcc
    19021 tatctgacag aggtacgaaa gctggttcgc tatcttcaaa tatgcgatgg taatatggaa
    19081 gaaggcagtt ttcgttgtga tgccaatata tctgtccgta aaaagggtga tatacaatta
    19141 ggtacgaaag tagaggtcaa aaaccttaac tctatacgta atgtaaaaag agctattgaa
    19201 tctgaagcaa aaagattgat taatattctc gaaaccggtg gaacggtaaa acagcaaaca
    19261 agaagctttg atgccaatac cggtaacaca ttctcaatcc gggacaagga ggatgcagat
    19321 gattaccgct attttgccga gcctgatctt acaccctttc atatacagga tgagtttatt
    19381 gagaggataa gaaaatcaat tcctgtttta caggaagaaa gaataagaca ctatattact
    19441 gtgctgagct tgtctgaata tgatgcggct gtacttacag aagaaaaaga aatgtcagat
    19501 tattttgata cagttattca gcatactgat aattataaag gggctgctaa ctggatgctt
    19561 ggccctgtaa aatcctggct taatgataat aataaagaac cagcggaatt ccctctgcct
    19621 gcaaaacaat tagcatcagt tattacttta attgatgccg gtaaaataag tttctctgcg
    19681 gccgccacta aattattccc ggctttattg aagaaccctg aaaaagatcc ggaacagatt
    19741 gcactggaag aaagtcttat tcagcagtct gacatatctt ccctggaacc cattgtagac
    19801 agtgtgctgg agaaatttgc tgacaaagtg aaagaatata aaaaagggaa gaagggattg
    19861 ttgtcattat ttgtaggtga agtaatgaaa cagacaaaag gtaaagcgga tcccaggctt
    19921 acgaatgagt tattactcga aaaattaaaa tcataataat gaagaaatta gtagttgtat
    19981 tcactgcagt tatttttttg gcagcctgca agaataaatc atcggtcagc aaatttgaag
    20041 taaagggtac tgttgttaac agtacggcta aaatgatata cctggaagag atcccaatgg
    20101 ctaccatgca ggcagtagta gttgattcgg cgctgatcgg aaaggaaggg aagtatgctt
    20161 taaaaactgt tgccagcgaa gcgagggtat acaatttaag gcttgatcaa agtacttacc
    20221 cggtagcagc tgttattaac gatgtacctt ccataacact ggatgctaca ttcagcaaag
    20281 agaataatca gtttgtagaa gggtacgaag taaaaggttc tgaaataagc cagcaattga
    20341 aagagttcat ggtcagcttc aatacaaagc tgcaggctgt tttttatatt gcccgccagg
    20401 cagacagcct taataagata caggcacctg atagtgtttt caggaaactg gaagcagaaa
    20461 atgcaattgt agctggcgat ttgaaaacat tgacatctga tgcattgaag aaaagtacca
    20521 atccggcgtt tacaatgctt attcttggtt attaccagag tacagctaac aaccagggtt
    20581 tcggccttca gccatttgcc aacgaggaag taacaaaatt ggtcaatgag ctggcggtta
    20641 agttcccggc tcaccaggga gtggcttctg ttaaaaatat gttgcaggca caggcaaata
    20701 aaatgcaagg gatgatcggt caaacagcgc cggaaataac cttacccgat ccgaatggaa
    20761 aagaaattaa gctgagctct ttccggggaa aatatgtgct ggtcgatttt tgggccagtt
    20821 ggtgcaaacc ttgccgggaa gaaaacccaa cggtggtaaa agcatacaat aaattcaagg
    20881 ataagaactt tacaatactg ggggtttcat tggacaggcc cggtcaaaaa gcagaatgga
    20941 tgaaagcagt gatgaaagat aacctgacct ggacacaggt aagtgacctt ttgttctgga
    21001 acagcccggt tgttcaattg tataatatag aggggatacc ctataatgtg ttggtagatc
    21061 cgcagggaaa aatcattgct gaatcgctgc gtggttctgc gctggaggca aaactgggag
    21121 aagtactgaa ctaaaaccag ttatcatttt cctggaagtc tcaatcctat aaatagtaaa
    21181 tttatagatg cgacttaatt tctttgttcg ccgcatttta tttagtggcc tgcttttcat
    21241 ccaaacttcg ggttggggag ggcagtttat gggaggggag ataagttacc ggtttatcag
    21301 ccattctggt aatgaatatg tttatgaact tacttttaaa atttaccggc aatgttttaa
    21361 tcctgctgac cttgataacc cggtcattaa acttattaat tctacagatc taacccggaa
    21421 catacaccct atcatttacc gggaaattat tttggataat cccaccatta caacactgcc
    21481 ctatacttat aaaaattatt gttcaatcaa cgatccaaca agttgcatag aacttcgaac
    21541 gtacaccgta caggtggggc ttccccatag tcccgagggg tatacgtttt attatacggg
    21601 ctgttgccgg aatggattaa gtaatcttgt aagtgatgca ggtaatggag gcgcagaata
    21661 tgttaatacc atcccggtac cgggacaggc attaacctat gttacctata tcccttcaca
    21721 tgatagtgta gcccagaaca gcagccctgt tgctgtgaac gattcgatag tatatgcctg
    21781 caaggggaaa ccatttcgtt accagtttca gttttttgat gctgatggag attctctggt
    21841 aataaaaccg gccgactcat ttcaaaaaac aaatgctgct accactaatt tttttccaat
    21901 tttttttaag cccggctata gtaataatag ccctatgggg aatacaatag cattgaacga
    21961 agaaggtata ttaagcggca ctccgggtgt ggaagggaat tttgcaatag ccttgactat
    22021 tgaagaatac aggaatggca ttctgatcaa taaaagcaga aaggactacc aggtaaatgt
    22081 ggctgcctgt gaaatcaaga aaccacttga tgtggttaat tgtgatgata atatcgcaat
    22141 gttcctccat aacaacaatc cgggcaatat atatcattgg gattttggtg taacgaacat
    22201 aatttctgat acttctgatg atacagaacc aatatacctg tatccgcaaa atggcacctt
    22261 ccaggtacaa ctggtagtca ctaatcctaa cgggggatgt aaggattctg tattagtgat
    22321 agcaaaagtt tatgctggtg gattactagt ggattttgac tggagcggac aaacttgtgc
    22381 aggtaaaccg ctgactttta cagacagaac aacatcaact gccggcgccg tggcagaaaa
    22441 ctggacatgg cggatattga ataacaatac aacgattgga aacactccgt cagttaccta
    22501 tactcatgca gtatccggta ctatgcccta tccgctggct gtccggctgg tggttgagac
    22561 ggatctcggc tgtaaggatt caatagttaa agacatactc atctttgaga atgtatatgc
    22621 agatgcagga ccggacagga ttcttccttt tggccaggta taccagatgc agggaaacag
    22681 tacagctacc ggagttaact atcaatggtc accatctttt ggtttaagtg atccttttat
    22741 ccgccatcct gaagtacggg cagacaggga tattgagtac atattggaag ttaataatga
    22801 agtgggttgt tttgacaggg atacggttaa gttcaggtat atgaaaggcc cggatattta
    22861 tgtagcatca gcgtttactc ccaataatga tggcttaaat gatgtgctaa attttttccc
    22921 ggttggaatg gagaatgcta agttgaatat ttataaccgg tggggccggt tggttttttc
    22981 tacagcagat ttaagtcagg ggtggaacgg gaaagtaaaa gatcttcttc aggatacggg
    23041 tgtatatgcc tggatagtaa aggcaacaga ttataaaggg caatcaataa taaaaaaggg
    23101 aacagttctt ttgatccgct gatcagggat taaaaaaggc tcttcaattg aagagccttt
    23161 ttttaaaatg aaaatttcaa agctattaat aaccagggtt ctggtcgtag ttcacatttt
    23221 gttgtgtttc ttcgatcgga attggccaca ggaacctgaa gtttgaataa gcaagggcag
    23281 gtacagacct tgtaaaggag gcaccggcac agttaaacat agctgcggta gcaccaccgg
    23341 aaccaacttt ggaaggtata ccaccaccag caataggtgc aaagtcagga tcaagcgcca
    23401 accgatgaat atcaccaaaa cgttttcctt ctgccaggaa ctcaattctt ctttctttaa
    23461 gaattgcact gatcaatgca tttttatcag caaagctggc aacggtgaat tggtcagctg
    23521 gtgttgtaac agccctgttt cttacagcat tcagcaatgc aacacctctt gttgatacag
    23581 tagttccggc tgcttgcctg gcttcagctt cagccagcat caataatact tcagcatacc
    23641 tgatcattgg tgctgcatca gaagaattgg catagtcacg gtatttaaca gtgtaaattg
    23701 cattaacagg tgagccagat gtgcgataca atgcagatcg acgtaagtcg gagcacagcc
    23761 attgaggatc actccagaga accgggctca ctctcaccaa tcccctggca cccaatgcag
    23821 gatcgcctaa catttgtggt aaggcaccgt taacctgggg attatctgta gcatcattct
    23881 taatggagaa aatgctttcg ttggttgtgt tattggcaaa agcgccatca acagctgcat
    23941 tcaatgtcca accacctatc ggacttacaa aaggtgctac ggctggaact aatttgtttc
    24001 cttcagtaat tacaccgggc caatcgcctt tatgcatttt aacccgcatc tttagagcaa
    24061 ttgcagcagc tttgctggcc ctgtatgttt ttatcgccgt tccggcagta tttacaactg
    24121 gtgtagcagg caggtttgct tcggcaaaat caagatctgc taagatgctg gcatagttat
    24181 ctgatacttt aatggtaccc cttttgatag cccttgcctg gtcagcagtg gctgcatcct
    24241 taattgcaaa gtccctgtaa ataataccca tctgggcacc tgctccgtca gcatagggcc
    24301 ttgaaaaatt aagtaccagt tcatgatgac ccatggcccg gagaaaacgg cattcagctt
    24361 catactgaag tgctacggca cttgtaatga ttccttttga accggcacct tttactccat
    24421 ctataacaag gttggctttg tttgccatac tgtaaatagt ctggaacata aaaccattat
    24481 taggggaagc attattatag gttgcttcat acgtaatctg gaagaaggca gcctggttca
    24541 gcatatcttc ccctcgcata tcgccctgtt caatacttgc agcaccaaaa ggataaccac
    24601 ggactgcacc accggcataa aagccggact gacaggcatc atatacacca tacactgata
    24661 attgaactgc atccgggttg gcaaaggcgg attcatctga ctgggctgta aacggcacca
    24721 ggtcgaggac ctttttttga caggaactca acgataaagc tcctgctatt aaaattgttg
    24781 atactattct gattttcatt tttttcattt tttggttatg aattagaaac caatattaat
    24841 accgaatgat attgtcctcg caggaggtat actattatag tcaagacccg tttcgctgat
    24901 attatcagga tctattccgg tataatctgt gatcacaaac aggttctgtc cctgaacaaa
    24961 gaaacggaat gatttaactg atgatttgaa catacggctt aataatgccg gggccacatc
    25021 atagctaaat gaaagattat ctaatttaaa gaaatcaccg tcttcaataa atctggtgga
    25081 agcctgctgg ttcaggttgg tgaagttgtc acggttatac cacaatcttg gtacatctgt
    25141 aacatcaccc ggtttctgcc acctgtttag aatataggtt ccattgttca taaaaccctg
    25201 gttcatcaat gattcctgct tggtgatatt catgatctta ttaccaccgg caaatctcca
    25261 caacatatcc atggtaaatt gcctgtaacg gacagtatta gtaataccgc caaagtactt
    25321 tggtaaagca cttcctaaaa tttgcctgtc accgccagcc agtgttccgg cnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nactttggta aagcacttcc taaaatttgc
    25501 ctgtcaccgc cagccagtgt tccggctgca ccaatagtag aactgtttaa atctgcaata
    25561 gcgaaatacg atgaattggt gatgttgccc attatcaggc tgccatccgc ttttctgtaa
    25621 actgggttac catttgaaga attaacgcct acaaattgat agccatataa tgcacctatc
    25681 ggctggccaa cacgaaggat attgtaatta ccagtaccat ttgattggat aacatcctgg
    25741 tttaagtaaa gactgagtac tttattagat tggttggtat aattaaaacc aaggtcaatt
    25801 gagaaatctt ttcttttaac tacagcaata tttaaagcaa gctctattcc tttattttcc
    25861 atacttccta tgttctggaa gatctggtta cccggcacac caaaagaagg tggctgggga
    25921 gcggctaata ccaggttatt gtttttgttt ttgaaatagt caaaaacaaa agttacacgg
    25981 tctttccata aggagatgtc aaaaccgtaa tcgattttct cgtttgtttc ccattgtaaa
    26041 gcagcatttc ctgattgaga agcagcaata ccactgatgg ctccatatgg agcgagacca
    26101 tagagattaa gatatgggaa aagaccacct gcgatagcag aattacccac tttagcccag
    26161 gaagccctga ctttgaaatc gctgatcact ttgtctaagc cactggtgct ccagaaatct
    26221 tcttgagata ctctccagcc aattgaaccg ccggggaaac taccaaaacg attctcttta
    26281 gcaaatcttg ataatccatc tcttctgaaa cttgcctgta aaaagtattt gccaccatag
    26341 tcataattga tacggccaaa ataagagaac aggcccgggc cctgtgaata gctaccagcc
    26401 gataactgtg ttacataact tccgctgata acattctgtt gctggaaaaa ggcatcggct
    26461 acgttggtac ctgatgcaga gaaactattg ttttcctgct tctgcgcctc aattcccgca
    26521 gtaaggttga aaccgtgttt tccaaatgtc ttactggcat tgatgtattc ctgtgctgtc
    26581 cagcgcatag tatttgctgc ctggttactt acccggccca atacactgcg gccatcacca
    26641 tgtttggcgt cctggcggaa aaattcatcc agtgttatgt aatcaatacc aattctaatt
    26701 gtattagtta accagcttgt aggtttgatc tcaataccaa aattgttgac aaccctgtgt
    26761 tttttagagc ggaatttatt ttttgctaat acatagccga tattaacata gttgttttcg
    26821 atcaggcggg tatttgcatc agaacctaat gcaccaccat caggtgtaat attgtaaaaa
    26881 tcaaactggg gaagacttgg gttaaataat ctaacgtttg gcagagccct caaagctgcg
    26941 gccacactac ctgataaggc gttaccacca ttattctggt ccttatcaaa tgcccttgat
    27001 aaagtaatgt agttcatcac cttcagccat ttgtttactt tatgttcgat gttggccctg
    27061 atattataat tctctgaagt gtttgttttt accaaacctt cctggtaacg gtagttaaaa
    27121 gaaaggaaat aagtggtacg atcattaccc ccgtctaatg acaggttatg cgtactggct
    27181 tgagaagtag gtctgaatac aacatcctgc cagtcagtat tcgtattctc tgagttcatg
    27241 aaagcgccat tggccaatcc tgcagcacta agcttggcat tgacaatggt tacaaattgc
    27301 tgtgcattca acagatcaaa tcggttgagg ggttttgcaa aaccgaaaac tgcactgtag
    27361 ttgaagtttt gccggccaga cttacctttc ttggtagtta tcatgataac accattggat
    27421 gcctgggagc catatatcgc tgtggcacta ccatctttca acacatcgat tgattcaata
    27481 tcggcaggat taatatttgc cagtacgttg tcgtttgtga atccggcaaa tcctccggaa
    27541 aacgtaggaa taccatctaa tacgatcaat ggaccgcggg caccattgat ggagtttaca
    27601 ccccttatac gaatacgagg tggttcattt accagtccgc ttgggttggt aactaataca
    27661 cctgcagaac gaccgcctaa ttgtttgtca atactggtac tgaccaacgg agcaatattc
    27721 tcaggattga ttttactgat agcggctgta gcctgtcttc tttgttgagt accataacca
    27781 accactacca cttcctgcat gattttatca tcgcctttca gtgatacact tattgttgtt
    27841 tgtgaaccaa tgctgacttc ctgggtaatc atgtcaaccg aggaaaagat gattgcccgg
    27901 gcattagcag gaactgaaag tgtaaaggta ccatcagctt tagtagtggt accaacagtt
    27961 gttcctctta ccataactga agcattggct accgggttac ctttttcatc ggtaaccata
    28021 ccggtaatgg tacgctgcgc cattgcctgt gtggcaaaaa acacaactcc caaaaggagt
    28081 agatacagtt ttctcatgtg cattttagtt ttagtatata aatgaaatcc aagcatcctg
    28141 aaccagtcga atactcaggg ggcttgaaat taaagaacct tacacgaaaa attcaaaaaa
    28201 tcgttgactt tttgctttaa taatctgtta gttgaataat accttaaccc ggactgcgaa
    28261 ctccgtcaac cctttgacaa ttaaccagaa ccaattgctg cacttatccg gtagatttta
    28321 tggtc
//
LOCUS       DAORTI010000036        27851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1265_length_27851_cov_7.088286, whole genome shotgun sequence.
ACCESSION   DAORTI010000036 DAORTI010000000
VERSION     DAORTI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 27851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27851
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1265_length_27851_cov_7.088286"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLO70_06670"
     CDS             <1..989
                     /locus_tag="PLO70_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HQZ74180.1"
                     /translation="QLVYNITRTDSLNIFDNTYSKNTVSMYKTDSSVTNLDTLFNQRW
                     SDFFSSSKDIVIPQVDDPMDPFEGYGKLSGKKNILITEWGPYDFRSPIIWNTNPTDTS
                     TTMKFDLIGTNGKWKVKSYKGVENLSAMSGVFPATITAQKIEVGRADIAIELEYTGDK
                     ITTPFGERINAGKAYNFSYRKFFQPLNWEVLFYALDTTSHDPIKTGQLFSVYEKKAPF
                     KRDSVDRLEYAWWGGIKANGINYPQFITVATTSAILPKGEYELSVTWDDAVRIFIDDK
                     LIVDEWEPHKFKFDESPNRRIPLIISGKNQFRVEHLELGGFATLNLKMRLIK"
     gene            1107..1412
                     /gene="rpsJ"
                     /locus_tag="PLO70_06675"
     CDS             1107..1412
                     /gene="rpsJ"
                     /locus_tag="PLO70_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQZ74181.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAEKIVKTVRSTGAVVTGPIPLPTR
                     RKIFTVLRSPHVNKKSREQFQLATHKRLLDIYTSSSRTVDALSKLDLPSGVEVEIKA"
     gene            1680..2297
                     /gene="rplC"
                     /locus_tag="PLO70_06680"
     CDS             1680..2297
                     /gene="rplC"
                     /locus_tag="PLO70_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQZ74182.1"
                     /translation="MKGIIGKKIGMTSVFDPSGKQTACTIIEAGPCVVTQVKTKESDG
                     YNALQLSFGDKKEKQSTKAEVNHFAKAQTAPKKFSKEFRDFSLEKNIGETITLDIFAE
                     GDKVEVVGTTKGKGFQGVVKRHGFHGVGQQSHGQHDRQRAPGSLGNSSDASRVMKGVR
                     MAGRMGNDRVKMKGLKVVKIFAEKNYILVSGSVPGHIGSIVLIQK"
     gene            2354..2989
                     /gene="rplD"
                     /locus_tag="PLO70_06685"
     CDS             2354..2989
                     /gene="rplD"
                     /locus_tag="PLO70_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQZ74183.1"
                     /translation="MKVEVLNTKGKATGRSVELPEEIFGAEPNNHVIYLAVKQYLAAQ
                     RQGTHKVKTRAEVQGASRKLHRQKGTGGSRKGNIRNPLYKGGGTIFGPKPHSYDIKLN
                     RKVKDLAKISALSYKAKDNAIVIVEDITMDSPKTKSFMEIMGSLKVADKKAMFIMPEY
                     NDNVQLSIRNVPSVLGVLLSDINTYDIVNSEVLVLTESAAKIFADDEKAEA"
     gene            3015..3314
                     /gene="rplW"
                     /locus_tag="PLO70_06690"
     CDS             3015..3314
                     /gene="rplW"
                     /locus_tag="PLO70_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQZ74184.1"
                     /translation="MKLSEVLIKPILTEKANAQQDKLRRYAFKVARKANKLEIKKAVE
                     SFYGVTVTGVNTAVVPGKNKSRFTKSGVISGRKPAYKKAFVTVAEGENIDLYSNI"
     gene            3375..4211
                     /gene="rplB"
                     /locus_tag="PLO70_06695"
     CDS             3375..4211
                     /gene="rplB"
                     /locus_tag="PLO70_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQZ74185.1"
                     /translation="MALRKFKPITAGTRWRIGNAYAEVTTNVPEKSLVEAKPRTGGRN
                     SSGHLSMRYRGGGHKKKYRIIDFKRNKTGVATVESIQYDPNRTAFIALLNYTDGEKRY
                     ILAPQGLQVGAKVESGADVAPEVGNALPMKNMPLGTNVHNIEMQPGQGGKLARSAGSS
                     AQLTNKEEKYAILKMPSGELRKVLINCWATVGVVSNSDHFLQSMGKAGRNRWKGIKPR
                     NRGVAMNPVDHPMGGGEGKASGGQPRSRNGQYSRGLKTRTKGKGSDKMIIQRKNGSKL
                     AK"
     gene            4243..4512
                     /gene="rpsS"
                     /locus_tag="PLO70_06700"
     CDS             4243..4512
                     /gene="rpsS"
                     /locus_tag="PLO70_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQZ74186.1"
                     /translation="MARSIKKGPYIATHLENKVLAMNEGKNKKGVIKTWSRRSTISPD
                     FVGHTFAVHNGNKFIPVYVTEFMVGHKLGEFAPTRQFKGHSKKEG"
     gene            4530..4877
                     /gene="rplV"
                     /locus_tag="PLO70_06705"
     CDS             4530..4877
                     /gene="rplV"
                     /locus_tag="PLO70_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQZ74187.1"
                     /translation="MEAVAKLRNYPTSPRKMRLLADLIRGQKVEKVLAELEHSPKHPA
                     VPLRKLVLSAISNWKQKNEGGDESQLVVKTIFVDGARTLKRMQPAPQGRGYRVRKRSN
                     HVTVIVDTKESKN"
     gene            4898..5680
                     /gene="rpsC"
                     /locus_tag="PLO70_06710"
     CDS             4898..5680
                     /gene="rpsC"
                     /locus_tag="PLO70_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQZ74188.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYATKKDYAGKLIEDHKIRTYLN
                     ARINKGGISKIVIERTLGKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKDDVQI
                     NILEIRRPELDAMIVGDTIARQIENRINFKRATKMAIASALRMGAEGIKIKLSGRLAG
                     AEIARSEEFKQGRVPLHTFRMDIDYANVFAQTVYGKIGIKVWICKGEVLAKRDLNPNF
                     IGGKNEVSDRRERRDDNRGGDRRDDRRGGGERRGGGGRDRRN"
     gene            5740..6162
                     /gene="rplP"
                     /locus_tag="PLO70_06715"
     CDS             5740..6162
                     /gene="rplP"
                     /locus_tag="PLO70_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQZ74189.1"
                     /translation="MLQPKRSKHRKMQKGRMKGDAKRGTTIAFGSFALKALDQHWITD
                     RQIEAARQALTREMKREGNVWIRIFPDKPITRKPAEVRMGKGKGNLEFWAAVVRPGRI
                     LFEIDGVSEQVARASLALAAGKLPIKTKFIVRRDLVSA"
     gene            6186..6395
                     /gene="rpmC"
                     /locus_tag="PLO70_06720"
     CDS             6186..6395
                     /gene="rpmC"
                     /locus_tag="PLO70_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQZ74190.1"
                     /translation="MSKKADFVKSIHGMNEQDLKARLEEDKQRLKKLEFAHAISPLEN
                     PMTIRGLRREIARLKTELKLKQQQA"
     gene            6409..6666
                     /gene="rpsQ"
                     /locus_tag="PLO70_06725"
     CDS             6409..6666
                     /gene="rpsQ"
                     /locus_tag="PLO70_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQZ74191.1"
                     /translation="MEARNLRKTRIGVVTSNKMTKTITVAVERKVKHPIYGKFVKKTT
                     KFHAHDDKNECSIGDVVKIMETRPLSKTKRWRLVEVVEKVK"
     gene            6780..7148
                     /gene="rplN"
                     /locus_tag="PLO70_06730"
     CDS             6780..7148
                     /gene="rplN"
                     /locus_tag="PLO70_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQZ74192.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQRYARIGDKIVVTVK
                     DALPAGGIKKGTVAKAVIVRTTNKLRRKDGSYIRFDDNAVVILNQSDEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            7184..7519
                     /gene="rplX"
                     /locus_tag="PLO70_06735"
     CDS             7184..7519
                     /gene="rplX"
                     /locus_tag="PLO70_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQZ74193.1"
                     /translation="MKARFKPKYNIRKGDNVVVITGDDKDLSKPRTVKEVLVEKGKVI
                     VEGVNIITKHTKPSAQNTKGGIVKKEAPISISNVMLWDAKAKAPAKVKREREDGKTVR
                     ISKKSGERI"
     gene            7548..8123
                     /gene="rplE"
                     /locus_tag="PLO70_06740"
     CDS             7548..8123
                     /gene="rplE"
                     /locus_tag="PLO70_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQZ74194.1"
                     /translation="MSTKTYSPRLADKYKKEVVPALVKKFGYDSVMQAPKLEKICLNR
                     GVNGAVSDKKLVDIALDELTTISGQKAVATMSKKDISNFKLRKNMPIGARVTLRGDKM
                     FEFLDRLIAVALPRVRDFKGVNEKAFDGRGNYTLGVTEQIIFPEIDIDKVNKITGLDI
                     TFVTTANTDEEAYELLKELGIPFKNAKNNAN"
     gene            8140..8409
                     /gene="rpsN"
                     /locus_tag="PLO70_06745"
     CDS             8140..8409
                     /gene="rpsN"
                     /locus_tag="PLO70_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQZ74195.1"
                     /translation="MAKTSVKARQKKREKMVAQHAAKREELKKAGDWKALDEMPKNSS
                     KVRLKNRCQLTGRPKGYVRYFGISRIALRDMALNGKIPGLKKASW"
     gene            8479..8877
                     /gene="rpsH"
                     /locus_tag="PLO70_06750"
     CDS             8479..8877
                     /gene="rpsH"
                     /locus_tag="PLO70_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQZ74196.1"
                     /translation="MVTDPIADFLTRIRNAQMAGHRVVDIPASNLKKRMTEILYNQGY
                     ILKYKFEDDNKQGVIKIALKYDLATKQPAIQSMERVSRPGLRQYAKPADFRRVKNGLG
                     VAILSTSKGVMTDKEAKSQNVGGEVLCYIY"
     gene            8965..9519
                     /gene="rplF"
                     /locus_tag="PLO70_06755"
     CDS             8965..9519
                     /gene="rplF"
                     /locus_tag="PLO70_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQZ74197.1"
                     /translation="MSRIGKQPVVAPNGVTISVGKDNVVTVKGPKGELKQAIDRDITV
                     EVKDGQITFARPTDQIRHRALHGLYRSLVSNLVKGVTDGYERKLELVGVGFKAANQGN
                     VLDLALGFSHNIIFEVPKELKVATAQEKGQNPTITLEGIDKQLIGQVAAKLRGLRKPE
                     PYKGKGVRYVGEVVRKKAGKAAGK"
     gene            9583..9942
                     /gene="rplR"
                     /locus_tag="PLO70_06760"
     CDS             9583..9942
                     /gene="rplR"
                     /locus_tag="PLO70_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQZ74198.1"
                     /translation="MNPKVKSQRRQNIRYRIRRKISGLTNKPRLSVYRSNADIYAQLI
                     DDTKGETLAAASSRDKDIATQKLTKSEKSKLVGAAIARKATELGIKDVTFDRGGYLYH
                     GRVKSVADGAREGGLQF"
     gene            9974..10492
                     /gene="rpsE"
                     /locus_tag="PLO70_06765"
     CDS             9974..10492
                     /gene="rpsE"
                     /locus_tag="PLO70_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQZ74199.1"
                     /translation="MSKFNVNRVKAGDLELKDKVVAINRVVKTTKGGRAFSFSALVVV
                     GNENGVVGHGLGKAKEVQEAITKGIEDAKKNLIKVPVMKGTIPHDQWAKEGAAKVLIK
                     PAAHGTGVIAGGSMRAVLEAAGVTDVLAKSLGSANPHNVVKATFKALALLREPINVAK
                     TRTLGLKKVFNG"
     gene            10517..10699
                     /gene="rpmD"
                     /locus_tag="PLO70_06770"
     CDS             10517..10699
                     /gene="rpmD"
                     /locus_tag="PLO70_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQZ74200.1"
                     /translation="MKKIKITLVKSPIDRPERQKLTVQALGLNKTNSTKEVEATPQIL
                     GMLRKVNHLVKVEDVK"
     gene            10781..11230
                     /gene="rplO"
                     /locus_tag="PLO70_06775"
     CDS             10781..11230
                     /gene="rplO"
                     /locus_tag="PLO70_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQZ74201.1"
                     /translation="MKLHELRPAKGATHKVKRIGRGDGSGHGGTSTKGTKGAQSRTGF
                     KNKMAHEGGQMPIQRRIPKRGFKNPHRVEYKVINLGQVEQLAEKYNITEFSLENLYIN
                     GLIAQTDSVKILGNGELKGKFTFKVNAVSEKAKSAIEAAGGSVEIVK"
     gene            11303..12640
                     /gene="secY"
                     /locus_tag="PLO70_06780"
     CDS             11303..12640
                     /gene="secY"
                     /locus_tag="PLO70_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQZ74202.1"
                     /translation="MKKLIQTIKNIWSIEELRGKILFTLMMIAIYRVGAHIVLPGIDP
                     NQMEAGAESNTGILGLIDAFSGGAFNQASIFALGIMPYISASIFMQLMTVLVPRFQKM
                     QKEGESGRKKINQYTRYLTVIVTILQASAYVSYLQGMTQQTGGIMAGYASYFWLSTVV
                     VLTAGTLFVMWMGEKITDKGLGNGTSLIIMIGIIARLPQSFLQELSAKSVRNGQILVF
                     IIEIAILIAIIMGCILLIQGVRKVPVNYAKQIVGNRQFGGARQFLPLKVNSAGVMPII
                     FAQAIIFIPTIFANYNTQASGFLRAITDHTNVWYMLIYSVVVIGFTFLYTALIFNPKQ
                     IADNLKQNNGFIPGVKPGQPTTDYIGSIMDKITFPGALMLAFVGILPGIAQLLGVTQQ
                     FATFFGGTSLLIMVGVILDTLQQIETQLLMRQYDGLMKSGRIQGRQTTSAVQM"
     gene            12715..13536
                     /gene="map"
                     /locus_tag="PLO70_06785"
     CDS             12715..13536
                     /gene="map"
                     /locus_tag="PLO70_06785"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQZ74203.1"
                     /translation="MLIYKTDEQVEMMRQSAMLVSKTLAEMTAMLKPGITTLSIDKAI
                     GEFIRDHQSIPSFLNYNGYPFNSCISVNDVVVHGFPGKYELREGDIISIDIGVILNGW
                     HGDHAYTFAIGDPGEAVMQLIRVTKESLYKGIEKAVAGNRVGDIAFAIQEHTEKKYGY
                     GVVRELVGHGLGKDLHEDPQVPNYGRRGSGAKLKEGLVLAIEPMINLGRRDIYTEDDG
                     WTVRTKDGKPSVHFEHDVCVRKNKAEILSDYSIIEKAERANTNLFTQNQIAEPLA"
     gene            13555..14805
                     /locus_tag="PLO70_06790"
     CDS             13555..14805
                     /locus_tag="PLO70_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQZ74204.1"
                     /translation="MSSSKKLVVIGGGSAGFFCAVNAARLNPGLKVVIIEKTNKILSK
                     VKVSGGGRCNITHACFDITEMSKKYPRGSNFVKKAYHQFFTTDTIKWFEDRGVKLKTE
                     EDGRIFPVTDSSQTIIDCLLNEVNNYSVEIMMNAEVKSVVYKDSYFELMLSGFASERH
                     RLLTTDYLCIACGGYPKSSMFEWIKELGHSVESPLPSLFTFNIPGHPITKLMGITVEN
                     VRVKIVSSKLEQTGPLLITHWGLSGPAILKLSAWGAKELSAMNYEFKILINWLPVFNE
                     QQLAERFQSLRFELASQKILNKNPFGLPQRLWEYLLDRSGVSNEKRWADIPAREQNKF
                     IRNLCACEFDVKGKTTFKEEFVTAGGLKLNEIDHNSMMSKKVPNLFFAGEIMDVDGVT
                     GGFNFQHAWTSGYIAAKNIAAQSI"
     gene            complement(14810..15772)
                     /locus_tag="PLO70_06795"
     CDS             complement(14810..15772)
                     /locus_tag="PLO70_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQZ74205.1"
                     /translation="MLYNFIRLLARLAIKIFCRKIVINKPAMLNEKGPLLLACNHPNS
                     FLDSVILNTLFEQPVWSLARGDVFKKPFYAKLLTALKILPVYRTSEGVENLSENYKTF
                     DACIRIFKTNGIVTIYSEGKCINEWHLRPLKKGTARLAAKAWEENIPLAVLPVGLNYN
                     SFRLFGKNVFINFGDIINQDYFNQNEPDGLRHQSFNNKLQMQLEKLVFEIPKIDKKQK
                     QKLAIDQPLLKKLLLSIPALLGWLLHIPLYLPVKKLALSRTRGTDHFDSVLVAILLIT
                     YPLYIILSITLAWILTNCWWVIFFLLVAPFTAWSYVQIKPQLDK"
     gene            15972..17888
                     /gene="rpsA"
                     /locus_tag="PLO70_06800"
     CDS             15972..17888
                     /gene="rpsA"
                     /locus_tag="PLO70_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQZ74206.1"
                     /translation="MFENIIKKQLNADAQESATDISEAATATTTAPVEAVATAHDDFD
                     WSVDKRNLTSYSKEEKEKYDKVYDDTFVQLDDGQMMKGLVVGLTKTDVVLNIGFKSDG
                     LISLNEFRDLPNLAVGDEVEVMIVEKEDRDGHLNLSRRQARITRAWERIVEVHKTGEV
                     ITGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKINETIKN
                     AVVSHKALIESDIEAQRAEIMSKLEKGQVLEGTVKNITDFGAFMDLGGLDGLLYITDI
                     SWGRISHPSEVLKLDQKINVVVLDFDDEKKRISLGLKQLTPHPWDVLGENIVEGNVVK
                     GKVVNIEDYGAFLEIQPGVEGLVHVSEITWANTPINAKEFFKLGDEHEAKIVTLDKDS
                     RKMSLSIKQLSADPWNDIETKFAEGSRHTGLVKNITNYGVFVELAPGIGGMIHISDLS
                     WLKRFNHPTEYTKVGANIDVAILGIDKENRKLQLGHKQLEEDPWNTLQDTFAIGSLHE
                     GTVIRRDDKGATVQLPYGLEGFAPNRHLAKEDGKSIGADETAQFMVIEFDRNEKRIVV
                     SHTRIWEQGKVEEAEAVKKEVRAEADKTKKAVKNIQSKVEKATLGDLGVLADLKKKME
                     GNAEEGNSEEPKSE"
     gene            18195..18950
                     /locus_tag="PLO70_06805"
     CDS             18195..18950
                     /locus_tag="PLO70_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ74207.1"
                     /translation="MLRFFSTDRIPAHSKGVSFLILFILAFFISRGHTTVGRTTSGFT
                     GDKCLFDNFSSYTSRPDTGGGILPLHNFSLSVTLKENIVQLKWLAENEMNTERFVIQR
                     STDGANYTDIGFKNPSGPINILTEYNATDDILNFPANIVYYRVKAEDNRANYAYSNIV
                     PVRLFKISGINLWPNPFTTDLKISYDAPANSSLDIHMLDNSGRVIMNTSYSVNRGMNQ
                     LSLPGVGKLPSGFYFVRITDKSNNEIFAHKISK"
     gene            19080..19847
                     /locus_tag="PLO70_06810"
     CDS             19080..19847
                     /locus_tag="PLO70_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQZ74208.1"
                     /translation="MTSIIERLKHFNPVRKLVYFVVGAVSYPGLAIINKLKISGTENL
                     KGLPREKVLFVSNHQTYFADVITFLHIFCAVKWGKMNRLGIPYYLLNPFTRVNYVAAE
                     ETMKGSFISRLFLLAGGLTVKRTWRAEGKEVRRGLDPSDTRKISRALENNWVITFPQG
                     TTKPFAPGRKGTALIIKQVKPVVIPVVISGFWRAFNKKGLKFKKKGSLLSVTFKKPLI
                     IDYEASTEVILAQLMDSIEQSKKYMLMGKHHWQTTDK"
     gene            complement(19856..20371)
                     /locus_tag="PLO70_06815"
     CDS             complement(19856..20371)
                     /locus_tag="PLO70_06815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQZ74209.1"
                     /translation="MEAKKSAQSLTIMTEIVLPNDTNVFGNLMGGRLMYWMDIAAAIS
                     AQKHCNAPVVTASVDNISFENPVKLGNVVHIEAKITRAFNSSMEVHMKVWGEDLTQQY
                     KYKSNEAYYTFVALDPNRKPRPVPQLIPETEEEKKLFDGALRRRQLRLILGGKMKPDD
                     ALELKALFIKD"
     gene            complement(20415..21542)
                     /locus_tag="PLO70_06820"
     CDS             complement(20415..21542)
                     /locus_tag="PLO70_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HQZ74210.1"
                     /translation="MDFPANYDEIVYRIDKIDPIQYSKTRNYIDGNITYLSPYISRGV
                     ISVKQVMEAVLAKGYKPYEITKFIQELAWREYFQRVWQHLEDDMFDDIRITRTGIRHR
                     QIPQAIITAKTGIESIDTAINDLYQTGYMHNHIRMYTASIACNIAKGHWQLPSQWMYY
                     HLLDGDLASNTCSWQWVAGSFSSKQYFCNQENINKYTYSKQKDTIIDKSYDETPLMEI
                     PNILTRTSSLQLTTALPGKTKPTFDHNLPLVLYNSYNLDPLWRNDIAANRLLVLEPSH
                     FKQYPVSEKVIRFIIELAANIDGLQLFIGEINEIPDLAKFPAIYSKEHPAFNYYPGKK
                     DEREWMFPGVTGMFNSFFSFWKKCEQQLKNMKAIQLELIRA"
     gene            21635..21913
                     /locus_tag="PLO70_06825"
     CDS             21635..21913
                     /locus_tag="PLO70_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HQZ74211.1"
                     /translation="MKDSLTEQDIDRIIEMAWEDRTPFEAIGYQFGLGEKQVIELMRK
                     EMKTSSFLMWRKRMCGRSAKHRQLRNASISRFKCSRQKAISGNKISKR"
     gene            complement(22040..23536)
                     /locus_tag="PLO70_06830"
     CDS             complement(22040..23536)
                     /locus_tag="PLO70_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HQZ74212.1"
                     /translation="MKAISIIFPHQLFSSNPSIDRSRPIYLVEEILLFNQYSFHKQKI
                     LLHRASMKKYEEMLLKKKLNITYIEAKDKLSDIRRLIKDLAKNKIEEIHYTDTADNWL
                     EKRIQLTAAENEIRLVKYPSPNFLTQPVETEEFFAKRKTYFQTDFYTWQRKKRNILVG
                     GTGQPTGGKWTFDTENRLKIPKNEIIPGIQFPKENKQIKEAREYVNKFFAANYGEIEI
                     FIYPTTHTEAEKWLDDFLQNRFKKFGIYEDAMAKNENFLYHAVLTPMLNIGLLDPQQI
                     INKAIEAADQYNVPLNSLEGFVRQIMGWREFIRIVYEREGIKQRTTNYWGFTRKIPSS
                     FWTGETGIHPIDRVIKKALKTGYSHHIERLMVVGNFMLLCEFDPDEVYRWFMEMYVDS
                     YDWVMVPNVYGMTQFADGGLMTTKPYISSSNYLLKMGDWNKGPWQEIWDGLFWRFMHV
                     HRDFFLKNPRLGMLIKIFDKMPVEKRNTHLKNAAQYLESLTDESKEKS"
     gene            23597..24313
                     /locus_tag="PLO70_06835"
     CDS             23597..24313
                     /locus_tag="PLO70_06835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ74213.1"
                     /translation="MESVKGKNILVVGATGGIGSKTAKLLAGSGANIFLAGRNAEKLD
                     LVAKEIGISAEKTFAVDISIPEQITALKENYFSVFPNLDILVNAAGIGIIKPVESLSG
                     DEFLRTLNYNLYGPFLLVKAFLPAMKEAKKGLIINIPGVLGKVPMAGAAAYSASKFGL
                     VGMMQSIREEIKRTDIRITNIFMGGVDSPFWDTIDLRVQREKMIQAEEAAKAIWFLCQ
                     QPVSGVVSEMVLQPFNHQAI"
     gene            24390..25619
                     /locus_tag="PLO70_06840"
     CDS             24390..25619
                     /locus_tag="PLO70_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQZ74214.1"
                     /translation="MEKSYPEISFDNTEFAFEYKSDKQLKKAHFLFSMMGKPWLVKIG
                     TRLAPWSINAGLPVKGIIRNTIFSQFVGGETLEQTAAVASMLGKYNVQVILDYGVEGK
                     EGENNFDQACEEFIRVINYAATQPNIPFMSVKVTGFARFSLLEKLDTIMHSNEGSLMK
                     RYLKAVDGLSKEEKEEWQRIRHRVMRVCEAAAAKKIGVSIDAEETWIQDPVDALTILM
                     MDSFNKESAIVFNTLQHYRHDRLKFLKDSYEAAVERKFILGAKLVRGAYMEKERRRAV
                     EKGYPSPIHPDKENTDRDFNAGVKFCIDNIERVSLVVASHNEYSNLYAARLLMEKGLP
                     LNHPHVHWSQLFGMSDNITFNLAHAGCSVSKYLPFGPIKDVVPYLMRRAQENTSVKGQ
                     TGRELGLIKKELKRRNP"
     gene            complement(25623..26015)
                     /locus_tag="PLO70_06845"
     CDS             complement(25623..26015)
                     /locus_tag="PLO70_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74215.1"
                     /translation="MLNSRRFYKELGNLFYAIAAADKKIRLEERNILHEEILYAWKHH
                     DHSMDRFGSDRAFLIEFEFETMEDEFETAENAFLSFVRFFKENENEIDIANRIKIFNS
                     ARHIAESVRKINADELGYLLRLKEMMKL"
     gene            complement(26147..27154)
                     /locus_tag="PLO70_06850"
     CDS             complement(26147..27154)
                     /locus_tag="PLO70_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74216.1"
                     /translation="MSSIVKAAFFAFNILISFQTVFSQSGLSDTTRLEKQISILNGKA
                     YASFPASAVVSPRVADIMAADPNENKETRIIFDIGNMKLVLFAQELFSLSGDRLFEDI
                     SKEEEPSFNFRRKELTNSDSFITVLSTPALFDTSAGAILVNSLLIKSPDNTVARIDAY
                     ININAFYLKDEFSKLTENIFKTLTKGNRRISLEAKEETYKIPGTSATFNFKLPKNYFV
                     SLDEKYDFSIFKINKYKNLSDTTYTSITIYTGRYPTFFHKEYGFSDSVALKAKGTFLQ
                     KNIEWLYFKEDAQSFFLKEQIIPADELSQGLVMHVAVLSNKKEQLEELLKVAESIKII
                     R"
     gene            27346..>27851
                     /locus_tag="PLO70_06855"
     CDS             27346..>27851
                     /locus_tag="PLO70_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74217.1"
                     /translation="MQIIKSFLLSVLVMIFFFASCQKNQEPTSIDQAFIQSFRDGKFS
                     VLEKYLPTREFYKSLGKDIASRTDTQIDSFLSLNKQKLTESWKKMQENLTNGKIDPAK
                     ISIKETVVYNPYQQSVMQAMVIVYDYNGKTWDDVTLIVKQQNNESYLLEIPNPLSAFL
                     MTDSSMVN"
BASE COUNT         8846 a         4903 c         6182 g         7920 t
ORIGIN      
        1 accagctggt ttataatatc acaagaacag attcgctgaa tatttttgat aatacatatt
       61 caaaaaatac ggtatcaatg tataaaacag attcatctgt tactaacctt gatacacttt
      121 ttaaccagcg gtggagtgac tttttttctt catcaaagga tattgtaata cctcaggtgg
      181 atgatccaat ggatcctttt gaaggatatg gtaaactgag tggaaaaaaa aatattctta
      241 ttactgagtg gggaccttac gattttcgtt cacccataat ttggaataca aatccaacag
      301 atacttcaac gaccatgaaa tttgacttaa ttggtacaaa tggaaaatgg aaagtgaaaa
      361 gttataaggg agtagaaaac ctttctgcaa tgagtggggt attcccggct accattactg
      421 ctcaaaaaat agaagtgggc agagctgata ttgctattga actggaatat accggagata
      481 agattacaac accttttggt gaaagaatca atgctggaaa agcgtacaac ttttcttacc
      541 ggaaattttt tcagccttta aattgggagg ttctttttta tgccctggat acaaccagtc
      601 atgacccaat taaaacaggt caattgtttt cggtgtatga aaagaaagct cccttcaaac
      661 gtgatagtgt cgacagactg gaatatgcat ggtggggcgg gataaaagca aatggtataa
      721 attatcctca gtttattaca gttgcaacaa cttcagcgat acttcctaaa ggggaatatg
      781 aactttcagt tacctgggat gatgctgtca ggatttttat tgatgacaaa ctcattgtag
      841 acgaatggga acctcataaa ttcaaatttg atgaatcgcc aaaccgacga atcccattga
      901 ttatcagcgg gaaaaaccag tttagggtgg agcacctgga gttgggcgga tttgctactt
      961 taaacctgaa aatgaggttg ataaagtagt tcccaggggt tttaaataag tttgtccaca
     1021 ggggataaaa ttctttccta agtatttgga acgtataggc ttcaccctta cctttgcact
     1081 cccaaaagaa aattgggttt tcacacatgt cgcagcgaat cagaatcaaa ttacagtcct
     1141 acgatcataa tctggtagat aaatctgccg aaaagatcgt aaaaacggta cgcagtaccg
     1201 gcgcagtagt aacaggtcca attcctttgc ctacacgccg taaaattttt acagtattgc
     1261 gttcaccaca cgtaaataag aaaagtcgtg agcagttcca gttagcaacg cacaagcgtc
     1321 tattggatat ttacacgtct tcaagccgta ctgtagatgc actgagcaag cttgatttgc
     1381 caagtggtgt ggaagttgag atcaaagcgt agaataggag gcattagcct cgcactatta
     1441 gttcttataa attgatttaa aattttgcta actctcaatc ctctgccggc aggcaggtga
     1501 aaaaagagct ctggctgtta aacataaaac aagccattca gggtttgttt actaccggta
     1561 cttccccata aaggaaaagg tcggtggcaa acggggattg acccttcgtt gcacacaaag
     1621 tgatttcatc actgtaacga agaatgtccc aataatcctg caggcataaa ttgtaaaaca
     1681 tgaaaggtat tattgggaaa aaaattggga tgaccagtgt tttcgatcct tccggcaaac
     1741 aaactgcctg tacgatcatt gaggcaggtc cctgtgtagt tacccaggtt aagaccaaag
     1801 agtctgacgg gtacaatgct ctccagcttt cttttggcga taaaaaggaa aagcaatcca
     1861 ctaaagccga agtaaatcac ttcgcaaaag ctcaaacagc tccaaagaaa ttctctaagg
     1921 aattccgtga tttttctctc gaaaaaaata ttggtgaaac tataacactt gacattttcg
     1981 ctgaaggtga taaagtagaa gtagttggaa ccacaaaagg taaaggcttt cagggtgttg
     2041 taaaacgtca tggttttcat ggtgtcgggc aacagtctca cggtcagcac gaccgtcaaa
     2101 gggctccggg ttctctcggt aactcttctg atgcgagccg tgttatgaaa ggtgttcgta
     2161 tggccggtcg tatgggtaat gacagagtga aaatgaaagg tctgaaagta gtcaagattt
     2221 tcgctgagaa gaattatatc ctggttagtg gttctgtacc aggtcatatt ggttcaattg
     2281 ttttaattca gaaataagac agtgatttca attcccgccc tggcggtgat tggttcacat
     2341 aaaagataag aaaatgaaag ttgaagtttt aaatacaaaa ggaaaggcga caggcagatc
     2401 ggttgagcta cccgaagaaa tctttggggc cgagcctaac aaccatgtaa tctaccttgc
     2461 tgtaaaacaa tacctggctg cacagcgcca gggtactcat aaagtgaaga cccgtgctga
     2521 agtgcagggt gccagccgca aactgcatcg tcagaaaggt accggtggta gccgtaaggg
     2581 taatatccgt aacccattat acaagggtgg tggtacgatc ttcggcccca agccgcacag
     2641 ttacgatatc aaattgaacc ggaaagtaaa agacctggca aaaatatctg cactcagcta
     2701 caaagcgaag gataatgcaa tagttattgt tgaagatata accatggatt ctccaaaaac
     2761 aaaaagtttc atggaaatca tgggtagtct aaaggtagca gataaaaaag cgatgttcat
     2821 catgccggag tacaacgaca atgttcagtt gtcaatccgt aacgttcctt ctgttctcgg
     2881 tgtgttgctg agcgatatta atacctacga cattgtaaat tctgaagtac tggtgttaac
     2941 tgaaagtgcg gcaaagattt ttgctgatga tgaaaaagca gaagcttaat ttaaaacatt
     3001 ctaaagagta agagatgaaa ctttctgaag tattaataaa accgattttg acagaaaaag
     3061 cgaatgcgca acaggataag ctgcgtcgtt atgcttttaa agtggcaaga aaggcaaaca
     3121 aactggaaat caaaaaagca gtagaaagct tttacggggt aactgtaact ggtgtgaaca
     3181 cagcagtggt tcccggcaaa aataaaagcc gctttaccaa gtctggtgta atctcaggtc
     3241 gtaaacctgc ttacaagaag gcttttgtaa ctgtagctga gggtgaaaat attgatctgt
     3301 attcaaatat ttaatcctta tttaatgaat ggcttcaaca gccgctaatg ttgaactaaa
     3361 aaagaatttg aaaaatggca ctaagaaaat ttaaacctat tacagccggt acccgttgga
     3421 gaatcggtaa cgcctatgct gaggttacta ccaatgttcc tgaaaaaagc ctggtagaag
     3481 ctaagccaag aacaggtggt cgtaactctt ctggtcactt atccatgcgc taccgaggcg
     3541 gtggacataa gaagaagtac cgtatcattg attttaaaag aaataaaacc ggtgttgcaa
     3601 ctgttgaatc aattcaatat gatccaaacc gtactgcttt catcgcattg ctgaattata
     3661 ctgatgggga gaagcgttat atcctggctc ctcaaggttt gcaggttggt gccaaagtag
     3721 aaagtggtgc tgatgttgct cctgaggttg gaaatgctct tccgatgaag aacatgcccc
     3781 tgggtactaa tgttcacaat attgaaatgc aacctggtca gggtggtaaa ttagcccgca
     3841 gtgccggatc ttctgcccag ctgactaaca aagaagaaaa atatgctatt ctcaaaatgc
     3901 cttccggtga gttgagaaaa gtgttgatca attgctgggc aacagttggt gttgtaagta
     3961 atagcgacca cttccttcaa agtatgggta aagcaggtcg taacaggtgg aaagggatca
     4021 aaccaagaaa ccgtggtgtg gcaatgaacc ctgtggatca cccgatgggt ggtggtgaag
     4081 gtaaagcttc cggtggtcag cctcgtagca gaaatggtca gtactccaga ggtttgaaaa
     4141 ctagaacaaa aggaaaggga agcgataaga tgatcatcca gcgtaagaat ggaagtaagt
     4201 tggccaagta ataaaactat ttaacaatac aacgacataa acatggctcg ttcgattaaa
     4261 aaaggtcctt atatagcaac ccatcttgaa aacaaggtgc tggctatgaa tgaaggcaaa
     4321 aacaaaaagg gtgttatcaa gacatggagc cgtcgctcta ctatttcacc tgactttgtg
     4381 ggacacactt ttgctgtaca caatggtaac aagtttattc ccgtgtatgt aaccgaattt
     4441 atggttggtc ataaattagg tgaatttgca ccaacccgcc agttcaaagg acactctaaa
     4501 aaagaaggtt aatctagaaa ttaataacaa tggaagcagt agctaaactg agaaattacc
     4561 cgacatcgcc ccgcaaaatg cggttacttg ctgaccttat ccgtggtcag aaggtagaaa
     4621 aagtattggc tgagctggag catagcccta agcatcctgc agtaccgctg cgtaaactgg
     4681 tgttaagtgc aatcagcaac tggaagcaga agaatgaagg gggtgatgaa agtcaacttg
     4741 tggtaaaaac aatttttgtt gatggtgcca ggactttgaa gagaatgcaa cctgctccgc
     4801 agggacgtgg ttaccgtgta cgcaagcgca gcaatcatgt aacagtaatt gttgacacaa
     4861 aagaatctaa aaactaaacg aattaataac caataacatg ggtcaaaaag caaatccgat
     4921 tggtaacagg ttaggcatca tccgtggatg ggaatcaaac tggtacgcca caaaaaaaga
     4981 ttatgccggc aaactgatcg aggatcacaa aatcagaact tatctgaatg cccgtatcaa
     5041 taagggtggt atttcaaaga ttgttattga aagaacattg ggtaaactga tagtcactat
     5101 acatacatca aagccgggta tcattatcgg taaaggtggt ggcgaggttg atcgtattaa
     5161 ggaagagttg aagaaattaa cgggtaaaga tgacgtgcag atcaacatcc ttgaaattcg
     5221 ccgtcctgaa ctggatgcga tgattgtagg cgatacgatt gcccgtcaga tcgaaaaccg
     5281 tatcaatttt aaaagggcca ctaaaatggc tattgcttct gcgcttcgta tgggtgctga
     5341 aggtatcaag atcaaattaa gcggtcgttt ggctggtgct gagattgccc gtagcgaaga
     5401 attcaaacaa ggccgtgtac cccttcatac attccgtatg gatattgatt atgccaatgt
     5461 gtttgcacaa acagtatatg gtaaaatcgg tatcaaagta tggatttgta aaggtgaggt
     5521 tttagcaaag cgggatttga accccaactt cataggtggt aaaaatgaag taagtgacag
     5581 aagagaaaga agagatgata accgtggagg tgatcgtcgt gatgacagaa gaggtggcgg
     5641 tgagagaaga ggtggtggag gaagagacag aagaaattaa ttgtataaac aagaatcctg
     5701 tctgccctag ggcaggcaag taactcgaaa ttataaacaa tgttacaacc gaaaagatcg
     5761 aagcacagga aaatgcagaa aggtcgcatg aaaggcgatg ctaaaagagg tactaccatt
     5821 gcatttggct cttttgcttt aaaggcgctg gatcagcact ggataactga tcgtcagatt
     5881 gaagctgccc gtcaggcatt gacccgtgaa atgaaaaggg aaggaaatgt atggatacgg
     5941 attttccccg ataagccaat cacccgcaaa cctgcagaag taaggatggg taaaggtaaa
     6001 ggtaacctgg aattctgggc tgcagtagta aggccgggtc gtatcttatt tgaaatcgat
     6061 ggtgtaagtg agcaggttgc ccgtgcatca ttagcgctgg ctgccggtaa gctgccaata
     6121 aaaacaaagt ttattgtacg ccgtgatttg gtttcagcgt aataattaat aaatcgtaaa
     6181 gcgatatgtc aaagaaagca gattttgtaa aaagtattca cgggatgaat gagcaggacc
     6241 ttaaggctcg tttggaggaa gataaacaac gtttgaaaaa actcgagttt gcgcatgcta
     6301 tttctccgct ggagaatccc atgaccatcc ggggattgcg tcgggagatt gcccgtctga
     6361 aaacggaatt gaagttaaag caacagcaag cataataaag ttataaaaat ggaagcaaga
     6421 aatttaagaa agaccaggat tggtgttgta actagcaaca agatgacaaa gaccattacc
     6481 gttgcggtag aaagaaaagt aaaacaccct atctatggta aattcgtaaa aaagactacc
     6541 aagttccatg ctcacgatga taaaaatgag tgcagcattg gtgatgtggt aaaaatcatg
     6601 gaaacccgtc cgctgagtaa gacaaagcgc tggagactgg tggaagtggt ggagaaagtg
     6661 aagtaagaaa gtaacaggtt tactagctgt agagagccag gaaccgtatc agataaaaag
     6721 atagttatta attgctaaag gctcgcaact cgcagctgat agctcaaagc ttaagaaaaa
     6781 tgattcagca agaaagccgt ttaaatgttg cggacaacag tggtgccaaa gaggtacttt
     6841 gtatccgtgt actcggcaac agcggtcagc gttatgccag gattggtgat aagattgtag
     6901 taactgtaaa agatgcactg cctgccgggg gtattaaaaa aggtaccgtt gccaaagcag
     6961 ttattgtccg cactactaat aagcttcgtc gtaaggatgg ttcttatatc cgttttgatg
     7021 acaatgctgt ggtgatatta aaccaatctg atgagcctcg tggtacccgt attttcgggc
     7081 ctgttgcgag agaactgcgt gataaaggtt atatgaaaat tatctcttta gcaccagaag
     7141 tattataaaa ttaaaagggt aaaacccatt aacgacagat aaaatgaaag caagatttaa
     7201 accaaagtat aacatccgta aaggcgacaa cgtggtggtt attaccggcg atgataaaga
     7261 tttatccaag ccccgtacgg taaaagaagt tttggtagaa aaaggtaaag tgatagtcga
     7321 aggagttaat atcattacca agcacacaaa accttcagca caaaatacca aaggcggtat
     7381 tgtgaaaaag gaagccccta tcagcatcag taatgtaatg ctctgggatg cgaaagcaaa
     7441 agctcctgcc aaagtgaaga gagaaagaga ggatggtaaa actgttagaa tatcaaaaaa
     7501 atcaggtgaa agaatctaat tcttgcttgt attaataagt gaattatatg agtacaaaaa
     7561 catattcccc aagactggca gacaagtaca aaaaagaagt tgtacctgcc ctggttaaaa
     7621 aattcggtta cgattcagta atgcaggctc caaaattgga gaaaatatgc cttaaccgtg
     7681 gtgtaaatgg tgcagtatct gataaaaaat tggttgatat tgctcttgat gagctaacaa
     7741 caatcagtgg tcagaaagct gttgctacca tgtcaaaaaa ggatatttca aatttcaaac
     7801 tgcgtaagaa tatgcccatc ggtgcaaggg taactttacg tggagataag atgtttgaat
     7861 tcctggatcg tttgatagct gtagcacttc cccgtgttcg tgacttcaaa ggagtaaatg
     7921 aaaaagcttt tgatggtcgt ggtaactata cgctgggtgt aacagaacaa atcattttcc
     7981 cggagattga tattgataag gttaataaga tcacagggct tgatatcact tttgtgacaa
     8041 cagcaaatac tgatgaagag gcatatgaat tattgaaaga gcttggaatc cctttcaaaa
     8101 acgcaaaaaa taacgctaac taaaaagtaa taaacaatta tggctaaaac ttcagtaaag
     8161 gccaggcaga aaaagagaga aaagatggtt gcccagcacg cagctaaaag agaagagttg
     8221 aaaaaggctg gcgattggaa agctctcgat gagatgccga agaattcttc taaagttcgg
     8281 ttgaagaatc gttgccagtt aaccggtcgc cccaaaggct atgttcgcta ctttggaatt
     8341 tccaggatag cactgcgtga catggcatta aatggcaaga ttcccggatt gaaaaaagca
     8401 agctggtaag aaattaaatt aataaactaa acttatccaa ttattccgtc ttagacggaa
     8461 cgtaggaggg caataaatat ggtaacagat cctatagcag atttcctgac aagaatccgt
     8521 aacgcacaga tggcaggtca ccgtgtggta gatattcctg cttcaaacct aaaaaaacgt
     8581 atgacagaaa tcctgtacaa ccagggttat attctgaagt acaaatttga agatgataat
     8641 aaacaaggcg ttattaagat agccttaaag tatgaccttg ctactaaaca accggctatt
     8701 caaagcatgg aaagggtaag tcgtccgggt ttgcgtcagt atgcaaaacc tgctgatttt
     8761 cgccgtgtga aaaatgggtt gggtgtagct atcctgtcta cttctaaggg agtaatgaca
     8821 gacaaggaag ctaaatcaca aaacgtaggt ggtgaagttc tttgctacat atattaatag
     8881 aaaatgttac tgatacatta agctgaaact atactaagct gaccggtcag taaacgataa
     8941 ataaaaaaga taaaataagt aattatgtct cgtataggta aacagccggt agttgcacca
     9001 aatggtgtta caataagtgt agggaaagat aatgtggtta ctgtaaaagg acccaaaggg
     9061 gagttgaagc aggcaattga ccgtgatatc acggtagaag taaaagatgg acaaatcact
     9121 tttgcccgtc caactgacca gattcgtcac cgtgcattgc atggtttgta tcgttctctg
     9181 gtgtctaacc ttgtaaaagg tgttacagac gggtatgaaa gaaagttgga gttggttggt
     9241 gtcggtttta aagctgctaa ccagggaaat gtcctagatc ttgctttagg cttttcgcac
     9301 aatatcattt ttgaagtgcc taaagaactt aaagttgcaa cagcccagga aaaaggccag
     9361 aacccaacta ttactcttga aggtatagac aagcagttga tcggccaggt tgcagcaaaa
     9421 ttgcgtggcc tacgtaaacc tgaaccttac aaaggaaaag gtgtccgtta tgttggtgaa
     9481 gtggtacgta agaaagccgg taaagctgca ggtaaataat taaagtaaaa tttaatcctg
     9541 gcatcccgat agcgatcggg aaggattgta aataaaaaag agatgaaccc aaaagttaaa
     9601 tcacagagaa gacaaaatat ccgctatcgc attcgcagaa aaataagcgg gttaacaaat
     9661 aaaccccgtt tgtctgtgta cagaagtaat gctgatatat atgcccagtt gattgatgat
     9721 acaaaaggag aaacgctggc cgctgcatca tcaagggata aagacattgc gacacagaaa
     9781 ctaacaaaaa gtgaaaagag caaattggtt ggtgctgcaa ttgcccgaaa agcaactgag
     9841 ctgggtatta aagatgtaac atttgatcgt ggaggttatc tttaccacgg tcgtgtgaaa
     9901 tcagtagctg atggagccag ggaaggtggt cttcaattct aaaaaaacat tcaaactttt
     9961 aattacagta taaatgtcaa agtttaacgt aaacagggta aaagcaggtg acctcgaatt
    10021 gaaagacaaa gttgttgcca taaaccgtgt tgtaaaaacc acaaagggtg gtcgtgcctt
    10081 cagtttctca gcattagtgg ttgtaggtaa tgaaaatggc gtggtaggtc atggccttgg
    10141 taaagcaaaa gaggtacagg aagcaataac aaagggaata gaggatgcaa agaagaacct
    10201 gattaaagtt cctgtaatga agggtactat ccctcatgat cagtgggcta aagaaggtgc
    10261 tgctaaagtt ttaattaagc cagctgccca cggtactggt gtaattgcag gaggttcaat
    10321 gcgtgccgta ttggaggctg ccggtgttac agacgtactg gctaagtctt taggctcagc
    10381 taatccgcac aacgtggtaa aagcaacttt caaagcattg gctttattgc gtgaaccaat
    10441 taacgtagca aagacccgta cacttggttt aaagaaagta tttaacggtt aattccgatt
    10501 acaaacgaat tgaattatga aaaagattaa aataacattg gttaaaagtc ctattgacag
    10561 accggaacgt cagaaattaa ctgtgcaggc gctgggtttg aataagacaa attctacaaa
    10621 agaagtagaa gccactccgc aaatactggg tatgcttcgt aaagtgaatc acctggtgaa
    10681 agtggaagat gtgaaataat tgaggaattc tcagttatat attaaatttt aaagcgagcc
    10741 ccgaatgctt tcggggcgat gagtaaaaaa taaaagcaac atgaaactac acgaattaag
    10801 acccgcaaaa ggggcaactc acaaagtaaa aagaattggt cgcggtgatg gttccggaca
    10861 tggaggcaca tcaacaaaag gtacaaaagg tgctcagagc cgtacaggct ttaagaataa
    10921 aatggcccat gaaggtggcc agatgcctat tcagcgtcgt attccaaaac gtggatttaa
    10981 aaatcctcat cgtgttgaat acaaagtaat caatctgggt caggtggagc aattggctga
    11041 aaagtataac attactgaat tcagtcttga gaacctatat atcaatggtt tgattgccca
    11101 aacggacagt gttaagatac ttggcaatgg cgagctgaaa gggaaattca catttaaagt
    11161 gaatgcagta agtgaaaaag ccaaatcggc aattgaagca gcaggaggtt cggtggagat
    11221 agtaaagtaa atttcgctac attgcaatga cgcattaaat gcgtctttta cgttttaaga
    11281 gctttaaact tttataatcc ccgtgaagaa attaatacag acaattaaga atatctggag
    11341 cattgaggaa ctgcgtggta agattttgtt caccctgatg atgatcgcta tctatcgggt
    11401 gggtgcacat attgtattac ctggtatcga tccaaatcaa atggaagcag gtgctgaaag
    11461 caatactggt atactgggcc ttattgatgc gttttctggc ggagccttta accaggcctc
    11521 tatatttgca ttaggtataa tgccttacat atcggcatca atttttatgc aattgatgac
    11581 agtgctggta ccccggtttc aaaaaatgca gaaagaaggg gagagtggca gaaagaaaat
    11641 taaccaatac acccgttacc ttacagtaat tgttactata ctccaggcat ctgcctatgt
    11701 atcttatttg cagggaatga cgcagcagac aggcggaata atggcaggct atgccagtta
    11761 tttctggtta tccaccgttg ttgttcttac agcaggtacc ttatttgtaa tgtggatggg
    11821 cgaaaaaata actgataagg gtttgggaaa tggtacatcc cttataatca tgattgggat
    11881 cattgcccgc ttaccacaat cattccttca ggaattaagt gccaagtcag ttaggaatgg
    11941 tcagatactg gtctttataa tcgaaattgc tatcctgatc gcaataatta tgggttgtat
    12001 tctcctgatc cagggagtta gaaaggtgcc cgtaaactac gccaagcaaa tagttggtaa
    12061 caggcaattt ggtggtgccc gacagttttt gcccttaaaa gtaaatagtg caggtgtaat
    12121 gcccatcatt tttgcacagg ctatcatttt tattcctaca atatttgcta attataatac
    12181 acaggcaagc ggatttttaa gagcaattac tgaccatact aatgtctggt atatgctaat
    12241 ttattctgtt gttgttattg gctttacttt cctttatacg gccctgattt ttaatccaaa
    12301 acaaattgca gataatctaa agcagaataa tggttttatt cccggtgtta aaccaggaca
    12361 acctaccact gactatattg gctcaataat ggataaaatc acttttcctg gtgcactcat
    12421 gctcgcattt gttggaatcc tgccgggtat tgctcaactt ttgggtgtta cgcagcagtt
    12481 cgcaactttc tttggaggaa catcccttct tatcatggta ggtgtaattc ttgacacatt
    12541 acagcaaatt gaaacccagc tgttgatgcg ccagtatgat ggattgatga agagtggccg
    12601 aatacaggga agacaaacaa cttctgcggt tcagatgtaa taaaattatt catacacaga
    12661 acccggtaag tatcccttgc cgggttttgc ttttttaagg atatttgcga atctatgctg
    12721 atatataaaa ctgatgagca ggttgaaatg atgcgacaaa gtgctatgct ggtaagcaag
    12781 accctggcag aaatgacagc aatgcttaaa cctggcataa caactttatc tattgataaa
    12841 gcaattggtg aatttatcag ggatcaccag tcgattcctt cttttctaaa ctataatggc
    12901 tatccgttta attcctgtat ttccgtaaac gatgtggtgg tacatggttt tccgggcaaa
    12961 tatgagcttc gggaaggaga tattatttct atcgatatag gtgttatatt aaacggctgg
    13021 catggtgacc atgcctacac gtttgctatt ggcgacccgg gtgaggcagt aatgcaattg
    13081 atacgggtga caaaggagtc attatataag ggtattgaaa aggcagtagc cggcaacagg
    13141 gtaggtgata ttgcgtttgc tattcaagaa cacacagaga aaaaatacgg atatggtgtg
    13201 gtcagggaat tagttgggca tggacttggt aaagatcttc acgaagatcc ccaggttcct
    13261 aactatggca ggaggggaag tggggccaaa ctaaaagaag gattggtact ggcaatagaa
    13321 ccaatgatta atcttggcag aagagatatt tatacggaag atgatggctg gaccgtaagg
    13381 acaaaagatg ggaaaccatc agtacacttt gaacatgatg tttgtgtcag aaaaaataag
    13441 gcggaaatac tatctgatta tagcattatc gaaaaagccg aaagggcaaa tacaaatctt
    13501 tttacgcaaa atcaaatagc tgaacccctc gcctgagggg tttgtttttt acttatgtca
    13561 tcttcaaaaa aactagttgt tattggcgga ggctcagcag gttttttttg tgccgttaat
    13621 gctgcacgac tgaatccggg attaaaggtt gtcattattg aaaagacaaa taaaatatta
    13681 tcgaaagtga aagtgagtgg tggtggccgc tgtaatatta cacatgcttg tttcgatata
    13741 actgaaatga gtaaaaaata tccgcggggt agcaattttg tcaaaaaagc atatcatcaa
    13801 ttctttacaa ccgataccat taaatggttt gaggacaggg gggttaaact aaagaccgag
    13861 gaagatggaa ggatatttcc tgtaacagat tcttctcaaa ctattattga ttgtctttta
    13921 aacgaagtga ataattacag tgtagaaata atgatgaatg cggaagtcaa gagtgtggtt
    13981 tataaggata gttattttga actaatgctc tccggcttcg catccgaaag acaccgcctt
    14041 cttactactg actatttatg tattgcctgt ggtggttatc ctaaatcttc tatgtttgaa
    14101 tggataaaag aactgggaca ttcagttgaa tcccctttgc cgtcattatt cacttttaat
    14161 attccgggac atccgatcac aaaattgatg gggataactg tagaaaatgt aagagtcaaa
    14221 attgtcagca gtaaacttga acagacagga ccgttgttaa taacacactg ggggttaagc
    14281 ggtccggcaa tattaaaact gagtgcatgg ggggcaaaag aattgtctgc catgaattat
    14341 gagtttaaaa ttctaataaa ctggttacct gttttcaatg agcagcaact agcggaaaga
    14401 tttcaatcac ttcgatttga actggcttcg caaaagatac tgaataaaaa tccatttgga
    14461 cttcctcagc gactttggga atatttactg gatcgttccg gggtaagcaa tgagaaacgc
    14521 tgggctgata ttcctgccag ggagcagaat aaatttatca ggaatctttg tgcctgtgag
    14581 tttgatgtaa aagggaaaac caccttcaaa gaagagtttg taacagcagg aggattaaag
    14641 ctgaatgaaa ttgatcataa ttccatgatg agtaaaaaag tgcctaacct tttttttgcc
    14701 ggggaaataa tggatgtgga tggagtgacc ggaggtttta actttcaaca tgcatggaca
    14761 agcggctata ttgcagcgaa aaacatagca gcacaaagta tttaatctgt tatttgtcca
    14821 attgtggttt tatctgcaca taagaccatg ctgtgaaagg tgcaacaagt aaaaagaaaa
    14881 taacccacca gcaatttgtg agaatccatg ccagtgtaat actaagaatt atatacaggg
    14941 ggtaggtaat aagcaatata gccacaagta cagaatcaaa atggtcagtt cccctggtgc
    15001 ggctcaaagc taattttttt acaggtaaat aaagcggtat atgtaataac catcccaaca
    15061 atgcaggaat ggaaagaagt aattttttta ataatggctg gtctattgca agcttttgtt
    15121 tttgcttttt atcaattttg ggtatctcaa aaacaagttt ttccagctgc atttgcaatt
    15181 tattattgaa tgactgatga cgcaaaccat ctggttcgtt ttggttaaag taatcttgat
    15241 taataatgtc cccaaaattg ataaatacat ttttcccaaa tagacgaaat gaattgtaat
    15301 tgagacctac cggtaaaacg gcgagtggaa tattttcttc ccaggctttt gcagcaagtc
    15361 ttgctgttcc cttttttaaa gggcgaagat gccattcatt gatacatttt ccttcgctgt
    15421 atattgttac tatgccattg gttttaaaaa tccggataca ggcatcaaat gttttataat
    15481 tctcagatag attttcaaca ccttcgcttg tacggtatac cggcagtatt tttaaagcgg
    15541 tgagcaattt tgcataaaat ggttttttaa aaacatcacc cctagcgagt gaccacactg
    15601 gttgttcgaa taaagtatta agtatgacag agtccaggaa agaattggga tgattgcaag
    15661 ccagtaataa cgggcctttt tcgttcagca tggccggctt attgatgaca atctttctgc
    15721 aaaatatttt tatagccagc cgggccaata gtctgatgaa attgtacagc aatacttagt
    15781 ttttggtgaa tatagttatt atttgagatg aagagcgaac agaaccctga ccagatcatc
    15841 gcaaggaacg ctgaatcaag gctttacaga ggagtggaaa cgtaaaaatc tctgccccgg
    15901 aaacagtccc aaaacattta tttagcctat ctttgctccc ccgaaaataa aacccttcgg
    15961 gactatatta aatgtttgaa aacattatta aaaaacaact aaacgctgat gcacaggagt
    16021 ctgctactga catttcagaa gcagctacag cgacaacaac tgcacctgtg gaagcagtag
    16081 ctactgcaca tgatgacttc gactggagtg ttgacaaacg aaaccttact tcttactcca
    16141 aagaagaaaa agaaaagtat gacaaagtgt atgatgacac ttttgtacaa ctggatgatg
    16201 gacagatgat gaaaggactg gttgtaggtt tgacaaagac tgacgttgtc ctgaacattg
    16261 gttttaaaag tgatggattg atttctctga atgaattccg cgaccttcca aaccttgcag
    16321 tcggtgatga agtggaggtg atgattgtag agaaagaaga cagggacgga caccttaacc
    16381 tgagccgtcg ccaggcacgc attacccgtg catgggaaag aattgttgaa gtacataaga
    16441 ctggtgaagt aattaccggt actgttactt caaaaaccaa aggtggtttg atcgtggatg
    16501 tttttggaat ggaaacattc ctgccaggat cacaaattga tgtgaagcct gtaactgact
    16561 atgatcagtt tgtaggaaaa acaatggaat tcaaagtagt taagatcaat gagactatta
    16621 aaaatgctgt tgtatcgcac aaagcactta tagaaagtga tatagaagca cagagagctg
    16681 agatcatgag caaactggaa aaaggacagg tattggaagg aactgtgaag aatatcacag
    16741 acttcggtgc tttcatggat ctgggtggat tagatggact gctttatatt accgatattt
    16801 catggggtcg tatttcacac ccatcagaag tgctgaagct tgatcagaaa ataaatgtag
    16861 tggtacttga tttcgatgat gagaaaaaac gcatcagcct tggcttgaaa caattgactc
    16921 ctcatccatg ggatgtttta ggagagaata ttgtggaagg aaatgtagta aaaggaaaag
    16981 tggtgaacat cgaagattac ggtgcattcc ttgagattca gccaggtgtt gaaggtcttg
    17041 ttcacgtttc agaaattaca tgggccaata caccgattaa tgctaaagag ttcttcaaat
    17101 taggtgatga acatgaagcg aagatcgtta cgctggataa ggatagtcgt aagatgagtt
    17161 tgtctatcaa acagctttct gctgatccat ggaatgatat tgaaaccaaa tttgctgaag
    17221 gcagccgcca tacagggtta gttaaaaata ttactaacta cggtgtattt gtagaattag
    17281 ctcccggtat tggtggaatg atccatatca gtgacctgag ctggttgaag cgtttcaatc
    17341 acccaacaga gtacactaaa gtgggtgcta atatcgatgt tgctatcctg ggtatcgata
    17401 aagagaaccg caagttgcaa ctgggacata aacaactgga ggaagatccg tggaatacat
    17461 tgcaggatac cttcgcaatc ggaagtttgc atgagggtac cgtgatccgc agagatgata
    17521 agggtgctac cgttcaattg ccttatggtc ttgaagggtt tgctcccaac cgtcacctgg
    17581 caaaggaaga tggcaagagt atcggtgccg acgaaaccgc acagtttatg gttattgagt
    17641 ttgaccgcaa cgaaaaacgt attgtggtat ctcatacccg tatctgggaa cagggaaagg
    17701 tagaagaagc agaagctgtt aaaaaagaag taagagcgga agctgataaa accaaaaaag
    17761 cagttaagaa catacagagt aaagtagaaa aagcaactct tggagattta ggtgttctgg
    17821 ctgatctgaa gaagaagatg gagggtaatg cggaggaagg aaattcagaa gaacccaaat
    17881 cggaataaga gggtttaatc tctataataa ggctgtccag aaaaagggca gccttttttt
    17941 tatagtttca atcgtagttt caacacaaaa gggtaaaata ttcgtataat tacgatgaaa
    18001 atttttgtcc tgaagcttgc atttactaat gcagagtatt ttctttgtat ctccatcctt
    18061 tgaagtgtac cggtttgtat tcagaagcta ataaaaggga ttactaatgt aaggaaagct
    18121 atttgatcac tgaatgcaaa acattggatc ttatatccgg aagagctttt attcgtaccc
    18181 aaaaaatcat gttcatgctg cgtttttttt ctactgacag aataccggct catagtaaag
    18241 gagtaagctt tcttatctta tttatcctgg ctttttttat ttcaaggggt catacaacag
    18301 ttgggagaac aacatccgga tttaccggag ataaatgttt atttgataat ttttctagtt
    18361 atacatcccg gcctgacacg ggtgggggaa tattaccttt gcacaatttt tcactttcgg
    18421 taacgcttaa ggaaaatatt gtacaattga aatggcttgc tgagaatgaa atgaatacag
    18481 aacgatttgt gatccagcga agtactgatg gagctaatta tactgatata ggatttaaaa
    18541 atccatctgg tccaattaat attttgactg agtataacgc aacagatgac atccttaact
    18601 tcccggcaaa cattgtttat taccgggtta aagcagaaga taacagggca aattatgctt
    18661 attccaatat cgttcctgtg cggctattta aaatttccgg aatcaatttg tggccaaacc
    18721 cgtttactac tgacctgaag atttcttatg atgctcctgc aaattcatcc cttgatattc
    18781 atatgcttga taattccggc agggttatca tgaatacatc ttacagtgtt aaccgcggta
    18841 tgaaccagtt gtctttaccc ggtgttggta aattaccttc cggtttctat tttgtccgga
    18901 taacagacaa gagtaataat gaaatattcg ctcataaaat ctctaagtaa gttataattc
    18961 ttctactgcc ggcgggtatg ctatcattgc tttcccggac aaggataaaa tgaacaatct
    19021 tgtttacttc atctacaatt ccttaatttg agatgtgaat tgcttgtcaa aactaacgca
    19081 tgacttctat tattgaaaga ctaaagcatt ttaacccggt aagaaagctg gtctactttg
    19141 tagtgggtgc tgtttcgtat ccgggattag ccattatcaa taagctaaaa atatcaggga
    19201 cagagaatct caaaggattg cccagggaaa aagtattatt cgtgagtaat caccaaacct
    19261 attttgcaga tgtaattaca tttctgcata ttttctgtgc agtaaaatgg ggtaaaatga
    19321 atcgcctggg tattccttat tacctcctga atccttttac aagagtaaat tatgtggccg
    19381 ctgaagaaac catgaagggg agcttcatca gtcgtttatt tttgctggct ggagggctaa
    19441 ctgtaaaaag gacatggcgg gcagaaggta aggaagtaag acgtggtctt gacccctccg
    19501 atacaagaaa aatttcaaga gctttagaaa ataactgggt aataaccttt ccccagggaa
    19561 ccaccaagcc cttcgcaccc ggcagaaagg gcacagcttt aatcattaaa caggtaaagc
    19621 ccgttgtcat cccagttgta atcagcggct tctggcgggc ttttaacaag aagggactga
    19681 agtttaaaaa gaaaggttct ctattgtcag ttacttttaa aaaacctctc ataattgact
    19741 atgaagcttc cactgaagta atattagcac aactcatgga ttctatcgaa caaagtaaaa
    19801 aatacatgct gatgggaaag catcactggc aaactacaga caagtaatga ataaatcagt
    19861 ctttgataaa tagtgctttt aattccagtg catcatccgg cttcatttta cccccgagga
    19921 tcaatcttaa ttgccttctt cttaatgcgc catcaaataa ttttttctct tcttctgttt
    19981 cagggatcaa ctggggaaca gggcggggct ttctattggg gtctaatgca acaaatgtat
    20041 agtacgcttc attacttttg tatttatatt gctgggtaag atcttctccc catactttca
    20101 tatgcacctc catggaggaa ttaaaagccc ttgttatttt cgcttcaata tgcaccacat
    20161 tgcctaattt gaccgggttt tcaaaggaaa tattatcaac ggaagctgtt actaccgggg
    20221 cattgcaatg tttttgggca gatatagcag ccgcaatatc catccaatac attaaccgcc
    20281 cacccatcag gttgccgaat acattagtgt cattaggcaa tactatttct gtcataatcg
    20341 tcaggctttg agcagatttt tttgcttcca tatactgtgt ttgggcaaag ataagcaggg
    20401 aaagccgtgg aagctcaggc tctgatcaat tccaattgta tggctttcat attctttaat
    20461 tgctgttcac attttttcca gaaactaaaa aaggaattaa acattccggt aacgccggga
    20521 aacatccatt ctctttcgtc ttttttaccg gggtagtaat tgaacgccgg atgttctttt
    20581 gaataaatag caggaaattt tgccagatca ggaatctcgt taatctctcc gataaaaagc
    20641 tgcaggccat caatatttgc tgccagctca ataataaacc ggatcacttt ttcgcttacc
    20701 gggtattgtt tgaaatggga gggctctaaa accagcagcc ggttggcagc aatatcattt
    20761 ctccacaagg ggtcaaggtt gtaggagttg tataatacca atggtaagtt atgatcaaaa
    20821 gtgggtttgg tttttccagg taaggctgtg gttaattgca atgaagaagt acgtgtcaat
    20881 atattaggga tttccatcaa gggggtttca tcatatgatt tatcaatgat tgtatctttc
    20941 tgtttactat aggtgtattt attgatgttt tcctggttgc agaaatactg tttggaagaa
    21001 aaacttccag ccacccattg ccagctgcag gtattactgg ccaggtcgcc atccagcaaa
    21061 tgataataca tccattgaga aggtagttgc cagtgtcctt ttgctatgtt acaggcaata
    21121 ctggctgtat acatacggat atggttatgc atgtaacccg tttggtataa atcattgata
    21181 gcagtgtcaa ttgattcgat cccggttttg gcagtaataa tagcctgagg aatttgacgg
    21241 tggcggatac ctgtccttgt tatacggata tcatcaaaca tatcatcttc caggtgctgc
    21301 catacccgtt gaaaatactc cctccaggca agttcctgga tgaatttggt tatttcataa
    21361 ggtttgtaac cttttgctaa tactgcctcc atcacctgtt tcaccgatat cacaccccgg
    21421 gaaatataag gagagaggta agtgatattc ccatctatat agtttcttgt tttgctgtat
    21481 tgtataggat caattttgtc aatccggtaa actatttcat cgtagttggc agggaaatcc
    21541 atgtttttga tttaaattat aataacaaaa tctggattgt ttggttgtag tatttaatta
    21601 tgtttaattc ttgtttcagt atttatatgg ttttatgaaa gattctttaa cagaacagga
    21661 tatagatcgt attatagaaa tggcatggga agaccggact ccgtttgagg caataggcta
    21721 tcaatttggt ctgggggaaa agcaggtcat cgaactgatg cggaaggaaa tgaaaacgtc
    21781 cagctttcta atgtggagga agaggatgtg tggacgatca gcaaaacaca ggcaattgag
    21841 aaatgcatcc atcagccggt ttaagtgcag ccggcaaaaa gctatatccg gtaataaaat
    21901 cagcaaacgt taggcagaga tcttatttct gtttatgcgg catttatcac tgcaatattt
    21961 cacctcttcc caaatttttt cccatttctt tcgccaggtg aaagggcggt tgcatacgat
    22021 gcaggttttt tgtggtaagt cagctttttt ctttgctttc atctgttaaa ctttctaagt
    22081 actgtgctgc attttttaaa tgagtgtttc ttttttcaac aggcatttta tcaaaaattt
    22141 ttatgagcat tcccagtctt ggatttttta aaaagaaatc ccggtgcaca tgcataaacc
    22201 gccagaatag tccatcccat atttcctgcc agggcccttt gttccagtcg cccattttta
    22261 ataagtaatt actgctgctg atataaggtt ttgtagtcat gagcccccca tctgcaaatt
    22321 gtgtcatgcc ataaacattg ggcaccatca cccagtcata gctgtccaca tacatttcca
    22381 tgaaccagcg atatacttca tcaggatcga attcgcaaag caacataaaa ttgccaacta
    22441 ccattaatcg ttctatgtga tgtgagtaac cggtcttcag cgcttttttg atcactctat
    22501 caattgggtg aatgcctgtt tcgcctgtcc agaaggagga gggaattttt ctggtaaagc
    22561 cccaataatt tgttgttctt tgttttatac cctctctttc atagactatt cggataaatt
    22621 cccgccagcc cataatttgc cggacaaaac cttccaaaga atttaacggc acattatatt
    22681 gatcagctgc ttcaatagct ttattgatta tttgttgcgg gtcaagtaat ccgatattga
    22741 gcatgggggt gagtacagcg tgataaagga aattttcgtt tttggccatt gcatcttcat
    22801 aaataccgaa ttttttaaat cggttttgca gaaaatcatc taaccatttt tctgcttctg
    22861 tatgagtggt tgggtaaatg aaaatttcaa tttctccata atttgcggca aagaatttat
    22921 tgacatactc tcttgcttct ttgatctgtt tgttttcttt aggaaattga atgccgggga
    22981 ttatttcatt ctttggaatt ttcaacctgt tttcagtatc aaatgtccat tttcctcctg
    23041 ttggttgccc ggttcctccg acaagtatgt ttcttttctt tctttgccag gtgtaaaaat
    23101 cggtttggaa gtaagttttc ctttttgcaa agaattcttc tgtttctact ggttgggtta
    23161 aaaaattcgg gcttggatac tttaccagcc tgatctcatt ttcagcggca gttagttgta
    23221 ttcttttttc aagccagttg tcagcagtat ccgtataatg gatttcttca atcttgtttt
    23281 tagctaaatc cttaataagt ctccttatat ctgataattt gtcttttgct tcgatatacg
    23341 ttatgttcaa tttctttttc aacagcattt cctcatattt tttcatagaa gccctatgca
    23401 ggagtatttt ctgcttatga aatgaatatt ggttgaataa caatatttcc tctaccaggt
    23461 atattggtct tgatcggtca atagaaggat tggatgaaaa gagttggtgg ggaaagatga
    23521 tggaaatagc cttcataata ttcagtaaaa aataaacaaa atagctatca ctttgtttat
    23581 taataaaaat ttatccatgg aatcggtaaa agggaaaaac atacttgttg ttggtgccac
    23641 agggggcatt ggttcaaaaa ctgcaaaatt acttgctggc agtggggcca acattttcct
    23701 ggccggcaga aatgcagaaa agctagattt agttgcaaaa gagataggga tttctgcaga
    23761 aaaaacgttt gccgtggata tttcaattcc ggagcagatc acagcattga aggaaaatta
    23821 tttttcagtt tttccgaatc ttgacatatt ggtgaatgct gccggtattg gtatcatcaa
    23881 acctgttgag agcctgagcg gggatgaatt tctgagaaca ttaaattata atttgtatgg
    23941 accctttctg ctggttaaag cctttttgcc ggcaatgaaa gaggcaaaaa agggtttaat
    24001 tattaatatc cccggtgtgc tgggcaaagt tccgatggcg ggtgcagccg cttacagtgc
    24061 cagtaagttt ggtctggtgg gaatgatgca aagtatccgt gaagaaatca agcgaaccga
    24121 tatacggatc acaaatattt tcatgggagg ggtggattcg cctttctggg ataccattga
    24181 cctgcgggtt cagcgggaaa aaatgataca ggccgaagaa gcagccaaag ccatctggtt
    24241 tttgtgtcag cagcctgtta gcggtgttgt gagtgaaatg gttttgcagc cttttaatca
    24301 tcaggctatc tgataaaact cattcagtta gttagcgact ttcaattaat tttgcggcaa
    24361 tcaatagtga tcggaccaac aattgccaaa tggaaaagtc atatcccgaa atttctttcg
    24421 ataacacgga atttgctttt gagtataaaa gcgataaaca attaaagaaa gcccattttc
    24481 ttttttctat gatggggaaa ccctggctgg ttaagattgg aacccgcctg gcaccctggt
    24541 ctattaatgc cgggttaccg gtaaaaggta tcattcgtaa tacaattttt tctcagttcg
    24601 tgggtggtga aacacttgag caaactgcgg cagtggccag catgctgggt aaatacaatg
    24661 tacaggtcat actggattat ggagtagaag gtaaagaggg ggagaataat tttgaccagg
    24721 cttgtgaaga attcatccgt gtcatcaatt atgcagctac acaacctaat attcctttca
    24781 tgagtgtgaa agtgaccggc ttcgcaagat tttccttatt ggagaagttg gataccatta
    24841 tgcattctaa tgaaggttca ttgatgaaaa gatacctgaa ggcagttgat ggtttatcaa
    24901 aagaggagaa agaagaatgg caacgcatcc gccatagagt catgcgggta tgtgaggcgg
    24961 cagccgctaa gaaaattggt gtgtccattg atgcggaaga aacatggata caggatccgg
    25021 ttgatgcact gaccatcctg atgatggact cctttaataa agaaagtgcc attgttttta
    25081 acacattaca gcactatcgc catgaccgtt tgaagttttt aaaggatagt tacgaagctg
    25141 ctgtggaaag aaaattcata ttaggggcca aattggttcg tggtgcctat atggaaaaag
    25201 aaagaagaag ggcagtagaa aaagggtatc cttcgccaat acatcctgat aaagaaaata
    25261 ctgaccgtga ttttaatgcg ggagtgaagt tttgtattga taatattgaa agggtttcac
    25321 tggtggtggc ctcacacaat gaatacagta atttgtatgc ggcccggcta ttaatggaga
    25381 aagggttgcc gctaaatcat ccgcatgtac attggagcca gttatttgga atgagtgata
    25441 atataacgtt taacctggcc catgcaggtt gttcagtgag taagtatttg ccatttggac
    25501 ctattaaaga tgttgttcct taccttatgc gccgtgcaca ggagaatact tcagtgaaag
    25561 ggcaaaccgg gagggagctg ggtttgatca aaaaggaatt aaaacgaaga aatccctgag
    25621 ggctataatt tcatcatttc tttcaggcgt agcaggtagc ccagttcatc agcattgatt
    25681 ttccggacac tttcagcaat atgcctggca ctgttgaaga tcttgatcct gttggctata
    25741 tcgatttcgt tttcattctc tttaaaaaat cttacaaagg ataaaaaggc attttctgct
    25801 gtttcaaact catcttccat tgtttcaaat tcaaactcaa tcagaaatgc tctgtcagac
    25861 ccaaagcgat ccatactgtg atcgtggtgt ttccaggcat acagaatttc ttcatgcaaa
    25921 atatttcttt cttccagtct tattttttta tcggcagctg caatagcata aaaaagatta
    25981 cccaattctt tataaaatct ccttgaatta agcatactta aaaataggaa agctacagct
    26041 ataaattaat tgacattaat cagctaatct gatgttgttt gtcaaatttt gtttatataa
    26101 agagggaatt gatctgctaa agtcaaaaag atgaagttaa aaatagttac cggatgattt
    26161 ttatactctc tgctactttt aataattctt ccagttgttc ttttttatta cttagtacag
    26221 ctacatgcat caccagcccc tggcttagtt cgtctgcggg gattatttgc tctttcagga
    26281 agaaactctg ggcatcttct ttaaagtaaa gccattcaat atttttctgc agaaatgttc
    26341 cttttgcttt caaagcaaca gagtctgaaa acccgtattc cttatgaaaa aaagtggggt
    26401 atcgtcctgt ataaatggta atactggtat aggttgtatc agaaagattt ttgtacttat
    26461 ttattttgaa aattgagaaa tcatattttt cgtccagcga tacaaaataa ttcttaggga
    26521 gtttgaagtt aaatgtggcg gaggttccgg gaattttata agtttcttct tttgcctcca
    26581 gggaaattct tctgttgcct tttgtcaacg ttttaaatat attttcagta agcttgctga
    26641 attcatcttt gaggtagaac gcattaatat taatgtacgc atcgatccgg gcaactgtat
    26701 tatcgggtga ttttatcaac aggctgttca ctaaaattgc tccggcactt gtatcgaaaa
    26761 gtgcaggagt agaaagtacg gtaatgaagg agtccgaatt ggtcagctct tttcgccgga
    26821 aattaaatga aggctcttct tccttcgata tatcttcaaa taatctatcc ccgctgagtg
    26881 aaaaaagttc ttgtgcaaac aataccagct tcatattgcc aatatcaaag atgattcttg
    26941 tttctttgtt ctcgttcggg tcagcagcca taatgtcggc taccctcggg ctaacaacag
    27001 cagaggcagg gaaactggca tatgccttcc catttagaat tgaaatttgt ttttcgagcc
    27061 gggttgtatc ggatagccca gattgtgaaa aaacagtctg gaaagaaata agtatattaa
    27121 atgcgaaaaa agcggctttt acaatggagc tcatgatatt tttttaatct gaatcaaaga
    27181 tacaggcaaa ggctgattcg ttgcatgcat gctttggttt gattggcaat aaccgggagc
    27241 cggctgcata caaccggaat ccaaaagtca atttccagga ataaataccc ggtaatgggt
    27301 atagaattta tttaaacatg gattttcttt atagtaaaat tgtttatgca gattattaag
    27361 tcgttccttt tgtcagtatt agtgatgatt tttttctttg catcctgcca gaagaaccag
    27421 gaacctacca gtattgacca ggcttttata caatcctttc gggatggtaa attcagtgta
    27481 cttgagaaat atctacccac ccgggagttt tataaatcac ttggaaaaga tattgcaagc
    27541 cgtacagaca cgcagatcga ttcatttcta agtcttaata agcaaaagct tacagagagc
    27601 tggaaaaaaa tgcaggaaaa cctgaccaat ggtaaaatcg atcctgccaa gataagtatc
    27661 aaagaaacgg tggtatataa tccctatcag caaagtgtaa tgcaggcaat ggttattgtt
    27721 tatgactata acggcaaaac ctgggacgat gtaactttga tagtgaagca acaaaataat
    27781 gaaagttatt tattggaaat tcctaaccca ttgagtgctt ttttaatgac agatagctca
    27841 atggtaaaca g
//
LOCUS       DAORTI010000037        26781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1368_length_26781_cov_10.099529, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000037 DAORTI010000000
VERSION     DAORTI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26781
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_26781_cov_10.099529"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1907)
                     /locus_tag="PLO70_06860"
     CDS             complement(249..1907)
                     /locus_tag="PLO70_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQZ74218.1"
                     /translation="MSQAVKTYPTILEKFFEYEKQCPDLLFLSEPVAGLARTFTWKQA
                     GDEVRRIVTALQSMSLPPQARIAILGKNSAHWILADLAINIAGFVSVPLYPNISAATL
                     QTILVHSEARVIFIGKLDNYEELLSGIPSSVCKISFPFYPKKDCLLWDELVKEYEPLS
                     STPMPDPYSLSCILYTSGTTGEPKGVMHTYFSHSYSLLTVFDALDNDFNAEIFFSYLP
                     LCHVAERMVVEYAGIFCGGTIYFPESPELFAKNLANAQPTVFLAVPRIWEKFQEEILK
                     KIPQKKLNIILSVPLLSAVLKKILKKKLGLSRAKHILTGASPIKASLLEWFATLGIII
                     QEAYGMTENMALSHINRKTTARFGTVGQSYPGVEVRLGKDREVQVKSPASMIGYYKEP
                     ELTAQSFEDGFLKTGDEGSIDNEGYLTITGRIKDQFKTSKGKYIAPAPIEKLLLESLC
                     FSQVCVVGSGLSHVLAICVLNKNPSVNEREPITRLLKEVLQNINSRLEHYEKLARLVI
                     VPEEWTVSNGLLTPTLKIKRSSIDKTFSGHYEEWLHANEDILFV"
     gene            complement(1904..2482)
                     /locus_tag="PLO70_06865"
     CDS             complement(1904..2482)
                     /locus_tag="PLO70_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQZ74219.1"
                     /translation="MLRRLFWTLLLKLNGWKTAVTPPAVTDKCIIIIAPHTSWQDFVI
                     GLAYRSVLRLKHAKFLGKKELFDGPFGFLFRWTGGLPVDRFSKQNMVDQVVDIFNRHE
                     KLTIAIAPEGTRKRVDRLRTGFYHIAKKAGVPIIMGGLDFKNKEMVFAKPFYAGEDEA
                     KDFEHIINFFAPIQGKHPELGLSHLQNEIPAS"
     gene            complement(2645..3568)
                     /locus_tag="PLO70_06870"
     CDS             complement(2645..3568)
                     /locus_tag="PLO70_06870"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HQZ74220.1"
                     /translation="MKKILSLIIFGLGFGAVAMAQETTEPEAKEAPKQKFTRATFNST
                     RIINMQSTEIVTPGSLQFMISHHFSPLWPEGGSSETGDNLAGLLGLNTGLAYTYLSFD
                     YSPTTWMNLGLAGAGRNRFEGWAKFKLLRQQTGAKNIPVSVAWYSMFNVNAKKDPAVA
                     SSWDKFSFMHQLLIARKFSDKLSLQLMPTLIHFNIVPYGPNNKNYVGSLGLGGKYKLT
                     ANKNITFEYARQLNMYENLLDKNGNIINYKPDLLSLGMEFNTGGHLFQFYIGNTTNSS
                     NIDQLARNSSSLKFSNWALGFTINRSMSLKK"
     gene            complement(3615..4247)
                     /locus_tag="PLO70_06875"
     CDS             complement(3615..4247)
                     /locus_tag="PLO70_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74221.1"
                     /translation="MKTKILLAAMATTMVMYMLSSCYRNREDILQVPVEKVSFRKDVV
                     PIVTAGPCGCHNLGNEPYAQFSHKDTVFYDVILARVNAFSVWVNGGKHPGLGTIDFTP
                     NQKFIIKKWIEQGPPYDSDDTGGGCTTGGETFTATILPIYNTTCKGGSCHGGGGVIAL
                     DSYSSMTSKRTILEAMMNSNGSTGHPGGALSLTSCTVNKFKSWITAGMPQ"
     gene            complement(4273..5475)
                     /locus_tag="PLO70_06880"
     CDS             complement(4273..5475)
                     /locus_tag="PLO70_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74222.1"
                     /translation="MKKVSFIQTTVLFLCGVFLTASVFQCRKDGALSKQFDRSFLETP
                     DSTIFSPFYDSLVISPSDPTAPDVNDIVKTRGVMGILKQYCGTANCHGGPISPKLDTY
                     LEVAKYITPGSPESSKLWEYITTNDFNKAMPPVNYVFSDGTPTNMTTRDKNIIYNWIK
                     NGAKENPDLADYRPTAIHLITTGCTSGNCHNQATTTGVWARSGLIPGITSADTIKWVT
                     NASSNTYCQLVNETKMKEVWGAYKDSVKRFYADTNANASFRPWKTFGSPTTVQSVRGP
                     LNDYDDIIMDIWYPKSLRTGTTVRYTDPVTGKKFYVSNTYLNASSTMVSRVDSTFHVA
                     NIHTNAWTTSLQGGMARADGNLSPSEIAIIKGWYFADPNIPDVYKYGKNNEGIFRYKK
                     TMNVITKR"
     gene            5766..5993
                     /locus_tag="PLO70_06885"
     CDS             5766..5993
                     /locus_tag="PLO70_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74223.1"
                     /translation="MGNNKKPADKSRRDFLSMFISADKKAASSETVKMLTPDGKLVQI
                     SKAVLDEITKKQKATNKEIYNWMNNPSKENS"
     gene            6027..6995
                     /locus_tag="PLO70_06890"
     CDS             6027..6995
                     /locus_tag="PLO70_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQZ74224.1"
                     /translation="MKQEEVNDKGRRDFLKTAALATVAATACGSLACSCNSKKAPVSD
                     KVKLLSPTGEIVEIDSAYLNHEEHMAIVSKLEAREGIAGKKFVMVVDLAKCKNARSCV
                     SACQKWHYRPKETEWLTVKLLQDSDKAAPYWFPKQCFHCDNPPCVKVCPVDATYKRQD
                     GIVLIDSDRCIGCKFCMAACPYSTRVFNWDEPELSLDIKNLAYSPETGIPAKVGTVEK
                     CDFCPDRAREGLLPPCVEACPNGVFYFGDENEDTVSNGLETVSFTQLIKDRSGYRFME
                     DLGTKPRVYYLPPKDREFPVEKGYQDADSAIKERFKDFKDILEAKE"
     gene            7036..8397
                     /gene="nrfD"
                     /locus_tag="PLO70_06895"
     CDS             7036..8397
                     /gene="nrfD"
                     /locus_tag="PLO70_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQZ74225.1"
                     /translation="MELNNYQQEAFEYQRKNIEKYGKRAWGWTIFYVLCILVGLYALY
                     LQIAKGHGVTGMRDNVVWGLFIVNFIFFIGLSYAGAIIAGLLHLSKVTWGKPIIRLAQ
                     FMTLVSVIIGPIFILLCVGRFDRMHHLFIYPRIQSPLTWDVMAVITFFVGSVLFLYLA
                     LIKDFAVYRDAKLNIPKWKQKLYKVLALGYRGAASQKRHLIISQNLMAVIMIPLAIMV
                     ASILSWIFGMTLRPGWHSTIFGPYFVLGAVYSGCGLLIVAMWFYRKLYKLDYYFTDKH
                     FTYLGYVMLILAAGYGYFTFSEYFTDWFASEKWNSELIHKLFSTSEYGWWTLFANAAG
                     IALPIAIVAIPATRKPGPITIAAFIMVIAMWVKRYLIIVPTLETTLLPMQDTRTEYVK
                     YAATWPEWALTIAGIASFLLFFTVMSKFVTVVPVSGLEDTHTVHEQHLLHPEPTLENT
                     SKA"
     gene            8417..9388
                     /locus_tag="PLO70_06900"
     CDS             8417..9388
                     /locus_tag="PLO70_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74226.1"
                     /translation="MKQVKFKITSTGFCRQLLLLAGMALFMLFTLPVWAQTDSTVQQE
                     TTEAPATAEEEEELVSPSIEFICIQKGDKSVDLKAAVKAKVQGKMRKLYGIKTTFYLI
                     TDSAEAELGSVISDRNGVALFNVKPEQVVANKEGNLHFKAVLGEVKKMESTEEEVTVK
                     RALLSITPVKEDSLYSVQVKLIDLSTGEETPVPETALGVFVKRSFNPMKIGEGTTDEN
                     GEASVEIPNNLPGDAKGNITLLVKLDESETYGYLEAAAVEKWGIPVSDKIEDQPRSLW
                     SSHPPLWMLITFIILMAAVWGHYIVIVYELFRLRKEQPNTADNATNS"
     gene            9425..9832
                     /locus_tag="PLO70_06905"
     CDS             9425..9832
                     /locus_tag="PLO70_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ74227.1"
                     /translation="MKTKALLLIVSIMAFGLFAFIPRTMVQPQPWPVPDAYKNKVNPL
                     KGDAESLVNGKALYNQHCKSCHGTKGKGDGPKASQLDTDCGDFTKSTFHSQTDGSLFY
                     KTFEGRKDMPAYKKKIPDQNDIWAIVNYMRTFK"
     gene            complement(9908..10801)
                     /locus_tag="PLO70_06910"
     CDS             complement(9908..10801)
                     /locus_tag="PLO70_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ74228.1"
                     /translation="MKLHQFVLAAAAPLLLFSCVSSKKFKTAKADYAKLETRYKTMES
                     DLSECSTEKATLAREKAALEREIALQNTRISDLNGQVDFLKANNNQALKQLEDLSVIT
                     STQAESIKKSMENIGAKDNYIQTLQQQMAHKDSLNMALVMNLKGAIGNMDDQDINIKV
                     DKGVVYIDISDKLLFKSGSYEVTEKAKGVLGKVAQVLKNQPDIEFMVEGHTDNVPYRG
                     SVLTDNWDLSVKRATSVVRILQKQYGLDPAKMAAAGRSEYRPVDDNGTKAGKAANRRT
                     RIVILPQLDQFFQLLEPGQGK"
     gene            11116..12975
                     /locus_tag="PLO70_06915"
     CDS             11116..12975
                     /locus_tag="PLO70_06915"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HQZ74229.1"
                     /translation="MSQQSNFFAMETAPARAELETWVKQIASFCEASNVHWCDGSKEE
                     YDSLCQLLVTKGTFIRLNPEKRPNSFACFSDPSDVARVEDKTFICSLRKEDAGPTNNW
                     MEPVQMKKKLQDLLQGAMKGRTLYVIPFCMGPLGSPLARYGVQITDSEYVVVNMRIMT
                     RMGREVLPYLQKKSYVKCLHSVGAPLEGNQKDSSWPCNPDVKYISHFPEERLIISYGS
                     GYGGNALMGKKCFALRIASVMGREEGWLAEHMLIMGVESPDKQKTYVAAAFPSACGKT
                     NFAMLIPPKEFDGWKVTTVGDDIAWIHKASDGGLYAINPEAGYFGVAPGTNYKTNPNA
                     MESIKANTIFTNVAITPDKDVWWEGLTKEPPAGLTDWHGQPWDPNSGKPAAHPNSRFT
                     APAYQNPAIDKDWDNPNGVPIEGFIFGGRRSTGVPLVYQSFNWNFGVYLATTMGSEMT
                     AAAFGEIGKVRRDPFAMLPFMGYHIGDYVNHWLQFGRNLPNAPRIFGVNWFRKDENGQ
                     FQWPGFGENIRVLKWIVERIRGNASSVESPIGWMPRYEDIDWTGLESFTKEDFAKIMT
                     VDREPGKQELLSHEELFERMYDKLPKEFFFMRELLLSALWRSPEHWGLQPERN"
     gene            13083..14753
                     /locus_tag="PLO70_06920"
     CDS             13083..14753
                     /locus_tag="PLO70_06920"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HQZ74230.1"
                     /translation="MPEWFHDLFTKTSIAQSVIIYGLVIAIGIWMGRIKIAGISLGIT
                     WVLFAGLIFSYFGVEIDSGTEHFMKEFGLILFVYSIGLQVGPGFWASLKKNALVNNLL
                     AFAIVATGVIITVILYTVSGNSIAVMAGVMSGAVTNTPGLAAAQAAVRDLHVTVADKS
                     LITLAYAVTYPLGVFGIIGSLLLLKKVFNIKIEKEQEFHRKLGVLRANRPVSVHLNLE
                     NKQLVGQPLRKLFDLLKEPVVVSRMYHKGEIITPTPDLILSENDVLLVVSSKQEMEQL
                     KLLIGRPSTMNLKTATDSDLISRMIVVTRSAVTHKRLGDIPELHQQEFTLTRLSRAGI
                     EMVPHGDIFLQLGDTIKVVGTEEGVKVVTQALGNSLKKLEVPDLAPIFMGIVLGVILG
                     SIPFHFPNMPVAVKIGMAGGPLIIALTLSRFGNLLYLNNYTTNSANLMIRELGISLFL
                     ASVGLSSGKNLSVAFADGRGWLWIGMGVIITVVPLLLIGFIARKYFKKTYFEVCGLLA
                     GASTDPPALAFATKLAGSDIPSVTYATVYPLTMILRIVAAQLLILLLL"
     gene            complement(14755..15108)
                     /locus_tag="PLO70_06925"
     CDS             complement(14755..15108)
                     /locus_tag="PLO70_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQZ74231.1"
                     /translation="MKKIYHLGNCTTCQAIIKEIKADQKGFVLQDIKFEKITPAQLDE
                     MKKMAGSYESLFSRRAMKYKELGLKDKKLVEKDYRKYILDEYTFLKRPVAIINDKIFI
                     GSEKKIVEALKNAVK"
     gene            15185..15913
                     /gene="cmoA"
                     /locus_tag="PLO70_06930"
     CDS             15185..15913
                     /gene="cmoA"
                     /locus_tag="PLO70_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HQZ74232.1"
                     /translation="MGKDEVFRDEIEKASDFKFGANVAKVFDDMVNRSVPFYGEIQRM
                     MAELAADHAREETFVYDLGCSTGTTMIGMDTMVNQQIKFIGVDDSQEMLDKCKSKLME
                     LGFSRPYELRCADLGQGVKIENASVVVLCLTLQFVRPVYRERLLQDIYQGLNPGGVLI
                     LVEKILAEESNFNRDFINYYYNYKRRNNYSEMEISQKREALENVLVPYKLSENISLLR
                     DKGFAHCEVFFKWYNFAGLIAVKK"
     gene            15919..16641
                     /locus_tag="PLO70_06935"
     CDS             15919..16641
                     /locus_tag="PLO70_06935"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQZ74233.1"
                     /translation="MVSIGNFFFKYRNWIFILFYGALFIPSWPLFSKEQLGESYYIWP
                     ISIGLIITCLGQLIRGLTIGLAYIVRGGKEGKPYAEGLVTEGIFTHCRNPLYVGNILM
                     LLGVGILANSLFYVGIMIPIFLFIYQAIVLAEENFLRGKFGIGFDEYCKNVNRWFPNL
                     KGIGKTFSSMEFNWKRWILKEHTTQFIWLIGITLLLLLNYPELTGHDEKFRNLLLGIV
                     LGALLLIYILIRYLKKTGKFQE"
     gene            16773..17369
                     /locus_tag="PLO70_06940"
     CDS             16773..17369
                     /locus_tag="PLO70_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQZ74234.1"
                     /translation="MIKTGNPIISIYTEMTPNPETMKFVANKLLYPGKSIDFPDAESA
                     KPSPLATELFGFPFIKAVFIASNFVTLTKTTETDWNDAIPSIRQFLKDYLEEGKAIIN
                     EEELAAVKQTSSNEVAADDDDVVKRIKELLDNYVRPAVEMDGGAIQFKSYEAGTVSLA
                     MQGSCSGCPSSMITLKAGIEGMMKRMIPEVKEVVAEAE"
     gene            complement(17624..18739)
                     /locus_tag="PLO70_06945"
     CDS             complement(17624..18739)
                     /locus_tag="PLO70_06945"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74235.1"
                     /translation="MTIAVLGAGMVGRAIALDLANDYTVTSFDVSAANLEVLIKRNTS
                     VQTVMADLSQFDAYKNWLSPFDLVVTAVPGFMGYKTLEAVINAGKNVVDISFFPEEAM
                     QLDALAKEKAVTVITDCGVAPGMSNFIIGRYNAEMKVKAFECYVGGLPKERIPPFEYK
                     APFSPVDVIQEYIRPARLVEQGSIVTKPALSDRELMLFDKVGELEAFNTDGLRSLIHT
                     MGHIPDMKEKTLRYPGHIDLIIALQQAGFFDITPIRVNGVSVVPLDFTSALLVNEWKP
                     GPDEEEFTVMKVVIKGEDKTVIYDLYDTYDPVTKISSMARTTGYTCNAAVNLITKKLF
                     TEKGVFPPELIGNRKDCFDFVIAYLKNRKVIWKKTEQ"
     gene            complement(18869..19009)
                     /locus_tag="PLO70_06950"
     CDS             complement(18869..19009)
                     /locus_tag="PLO70_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74236.1"
                     /translation="MICKDTIEDKILQLQEKKRALAKDIISDDTSFVKTLTREYVEYL
                     FS"
     gene            complement(19189..19833)
                     /locus_tag="PLO70_06955"
     CDS             complement(19189..19833)
                     /locus_tag="PLO70_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74237.1"
                     /translation="MRTTINILKTGLLITAAQFLLSSGCNKNSTTPCQNATYSFAVTC
                     NYSPEREIYNIGDTIFFESIFPKTLTSLLNPSTIVDYSNSTGIGGDIRIYYLDTITRQ
                     PLPGKDSFAFASIIGNFQERTGNQNSGINTLFFETSQNYHFKGAIICRKKGIYGFGVS
                     DLISNGIRGKNCTNAEFVMTVTNPNKHLHLHQYTLGVDPNDPMLQRIGYDFRVQ"
     gene            complement(19817..21952)
                     /locus_tag="PLO70_06960"
     CDS             complement(19817..21952)
                     /locus_tag="PLO70_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74238.1"
                     /translation="MKFINRIVILILATALINSCKKQYTNTETLQLDKTQFDYKNLKV
                     PNNFFKELPVPGVADPTNAPTSNLIENGTSPDPNNGDDPIILGQQVPNPYTIANMQQA
                     YLQLYGSTAPVPVTHLYIRYKPATQDQLATLLNQPNLELQDYPMDYKVLQDGDYYQDP
                     TLGTEDIGWLYSAVPANYTPVAGIQYEVIQQLHIPANDDLLLEGMAESLAGGAIYDDT
                     VIVADRYITRVDEPSDTLILPNRLPVPCEIDPCAPGCDPTGCGGGGGGGGGTYNPQIP
                     RGTIQVQDQRTCNTVTTPIVNVPVRQARIVCKRWFKIWTGYTNDQGQFLATKRFRNKV
                     KVILKTENNNAKVAKVRGIRLWQIAFPVKKRLGVFDQIAMANISYLFEKPNPTNAHDK
                     ELPYWVATTTHNSVLEYGQYAGEVGILNHPTKLNIIVTNWGFQRGAGAAPMWNKCHVL
                     NSELNLLQGFVQYFIAQPLLLSVPIVNLVEVLKNETDVIIGYAATVADYNCLLTSSTV
                     KAIVYHELGHTSHFAQAGCDNWQVYRTRISNELAETFGGDPYGDGTETNAGIVAVGEM
                     WGNHCEKWFSERHYTNPARETISLGQNGIYINDGSALANYGGYLTFSLAGLNANFASI
                     ENFDPNRAADAHRWIPQGLCYDLFDPRNDASFGGPLFDNVTGFTIQQCFNALQSDVRT
                     IPAFRDRLLQQNGNNQQVQVNALVNAYNY"
     gene            complement(22229..25993)
                     /locus_tag="PLO70_06965"
     CDS             complement(22229..25993)
                     /locus_tag="PLO70_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HQZ74239.1"
                     /translation="MALPHLIKYVYTHGTDEVIRRGKKIHAIGFVELVEYDDLFGSAV
                     FRVKDDSYSTYYKVYIQKFKDPKSTSLRCACPYNLGDICRHEAAALFQLQEMIDRGHL
                     QADNVKYDQRHTVAKMKLIDLKTLRLLCSPETLIDAETFLRTNKATIEYAENETVKAS
                     VKLDGQTYKVIIRKNEERNFDTSCDYEDENHPLCLPKMIVLLQLLNAYGANYFDSIRN
                     WDKEKNKLLEAYGYSLSDDLKGKFEFAYKDGKPFLRVLDVKIKRVAPVAAPTRPVLIP
                     QKEREPDEIPAVAEEAEANPSQRLGIVFNFNKKTYPRFTVDVVTGEPNDSNDAFAGKV
                     EKLDISRYVNTDQLPEGDRELLVMLRKLQDSEINKYISRNSPFSGIWENIIHQEEDDL
                     PDETKELMAEYLLPKLKKIFTESAGNPFVFLLPESKTFKTNNLEPLQLQAEDAKPHFI
                     IKKNSHYTIQCRVKAGSLEFDLGDNEAASPLFFLYNNQAYLWKNNEVIHLVEKFLPTG
                     KMNVAAEEWTSQLNKFILPLAKEHKVDFDKSLVQEIKEGNPEVKLFLMEKGDYLVFQP
                     SFSYKGYETKAKDRDEVIVSQGDKVLVVHRNREKENEFIQKLQNLHSNFVYNEDSATL
                     ALKGTDVLKNNWFFLFVDAMKDYKTPVFGFEALKNFRFNTAKPQTKIFISSNTDWFDA
                     KVDIVFGDQKVTVAEVKRALANKQQFVHLNDGTLGILPEEWLKKYSLLFRVGEGKTDS
                     LKLSRYHLSVVDELYETRDEEELVVKLEEKYEKLREFNKIKEIDPSDSLKKILRPYQV
                     AGYQWLNYLKDIKWGGILADDMGLGKTVQALSFLEHFKNDQGKLKALVVCPTTLIYNW
                     ENEIKKFTPSLTYRIHHGAVRTRAKEELIDHDVTITTYGTLRSDIKLLMSVDFDYVIL
                     DESQAIKNPSSKVTKAACLLNAKNRLCMSGTPLQNNTFDIFAQMNFLNPGMLGTMEFF
                     RQEFAIPIDKFGEQDRKDHLRKILYPFILRRTKEQVAKDLPEKQEMILFCEMEDEQRN
                     IYDAYRNDFRNQILGTIETQGIQKSQLTILQGLMKLRQICDSPAILNEQEKFDNHSIK
                     LDELARELSEDMGNHKALIFSQFLGMLALIRAKLEELGIKYEYFDGSTSAPDREKAIQ
                     SFQNNEEVRVFLISLKAGGVGLNLTAADYVYIVDPWWNPAVEQQAIDRTHRIGQTKNI
                     FAYRMICKDTIEDKILQLQEKKRALAKDIISDDTSFVKTLTREDVEYLFS"
     gene            complement(26167..>26781)
                     /locus_tag="PLO70_06970"
     CDS             complement(26167..>26781)
                     /locus_tag="PLO70_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQZ74240.1"
                     /translation="IASALYNGAKCVIPVDSVPKAIDISKNIGGIAAGERDGMIAEGL
                     QHGNSPLEYTREFIENKTLVLTTTNGTRLLQMALDRNADMIISGSFPNLSAVCDFLLA
                     EKKNVVLGCAGWKDRFNLEDTLFAGAVINRLTKEFTVHCDSSLMAETMYTKHKNKLIE
                     FAPKLTHYHRLVERFGLIEDIKFCLTPDVANVLPVYSNGMLISK"
BASE COUNT         7535 a         5611 c         5469 g         8166 t
ORIGIN      
        1 tgaatggccg ttattttact gcagacaatg tgcggaagaa taaacagacc ggtgtgtttg
       61 aagaaataaa atagctgtta aattttatat tcaggaattt cacagctatt cccccttaac
      121 ggttgaaact gatattggtt gtgtacaata ccaggctccc cgaacagtta ataaattaca
      181 gaaaccactt aacttgatgg cagcgggtga gcaggtttct ataggagatc agcacagcgt
      241 aaaaatattt atacaaacaa tatgtcttcg ttggcatgca gccactcctc gtaatgcccg
      301 ctgaaagttt tatcaatgct acttcttttg atctttaaag ttggcgttaa caaaccattc
      361 gacacggtcc actcttccgg tacaatgacc agcctcgcca gtttttcata atgctccagc
      421 cggctattga tattttgtaa aacttccttt aataaacggg taatgggttc tctttcattg
      481 acggaaggat ttttgttcaa tacacatatt gccaatacat gtgacaagcc agaaccaacc
      541 acacatacct gtgaaaaaca gaggctctcc agcaatagtt tttcaatggg tgccggggca
      601 atgtatttcc ctttgcttgt tttaaactga tccttaatcc tgccggtgat ggttaaatac
      661 ccttcgttat ctatggaacc ttcatctcct gtttttaaaa agccatcttc aaagctttgt
      721 gctgttaact ccggctcctt atagtaacct atcatagatg cagggctttt cacctgtact
      781 tccctgtctt ttcccaaccg cacttcaact ccaggatagg actgcccaac cgtaccaaaa
      841 cgagctgtcg ttttccggtt aatatgggag agggccatat tctctgtcat tccatacgct
      901 tcctgtataa tgataccaag tgttgcaaac cattccaaca gggaagcttt aatgggagaa
      961 gccccggtta gtatatgttt ggccctggat aaacccagtt tctttttcag tatttttttt
     1021 aataccgctg aaagcaaagg cacagaaaga atgatattca gttttttttg tggaattttt
     1081 ttcagaatct cttcctggaa tttttcccag atacggggga ccgctaaaaa aacagttggt
     1141 tgtgcattcg ccaggttttt agcaaataat tccggtgatt ccggaaaata aattgtgccc
     1201 ccgcaaaaga ttccggcata ttccactacc atccgttccg caacgtgaca aagtggtaaa
     1261 taagaaaaga agatctccgc attaaaatca ttgtcaaggg catcaaaaac ggtaagcagg
     1321 ctatagctat gcgaaaagta tgtatgcatc acccccttgg gctcaccagt tgtgccggag
     1381 gtatataaaa tacagctaag cgaatagggg tcgggcattg gagtcgaaga tagtggctcg
     1441 tattctttta ctaattcatc ccacaataag caatccttct tagggtaaaa gggaaagctg
     1501 atcttacaga cggaagaggg aataccggat aataactctt cataattatc gagtttgcca
     1561 ataaaaataa cccttgcttc actgtgtact aatatagttt gtaacgtagc ggctgaaata
     1621 ttaggatata acggaactga aacaaagcct gcgatattaa tagcaagatc ggcaagtatc
     1681 caatgtgcag aattttttcc aaggatggca atcctggcct ggggcggtaa tgacatggat
     1741 tgaagagcgg taacaatcct tcttacttca tcacctgcct gtttccaggt aaatgtccgg
     1801 gctagtccgg caacaggttc agataaaaat aggagatcag gacattgctt ttcatattca
     1861 aaaaactttt cgagaatggt gggatatgtt ttgacggcct ggctcatgaa gcagggattt
     1921 cattttgcaa atgtgataat ccaagttccg ggtgcttacc ctggatgggc gcaaaaaaat
     1981 taatgatatg ttcaaaatct tttgcttcat cctccccggc ataaaatggc tttgcaaaaa
     2041 ccatttcttt gttcttaaaa tctaaacctc ccataatgat tggaacgcca gccttttttg
     2101 cgatgtgata aaatcctgtt cgcaggcggt ccactctttt ccgggtacct tcgggagcaa
     2161 ttgcaatggt cagtttttcg tggcggttaa atatatccac tacctggtct accatatttt
     2221 gtttactgaa ccggtccacg ggcagacccc ctgtccatcg aaataaaaaa ccaaaagggc
     2281 catcgaataa ttcttttttc ccaaggaatt tggcatgctt caaccgaagc acacttctgt
     2341 aggcgaggcc aattacaaaa tcctgccagc tggtatgggg tgctatgata attatacact
     2401 tatcggtcac agccggagga gtaacggccg ttttccagcc attcagtttc aataataatg
     2461 tccagaagag tcgtctcagc atcgttagtt gttattacaa tattaagaat aattaatccc
     2521 ttgttccggt agtatatgta tgtaattcaa aaatattttg atgccggtta ctggtagtcg
     2581 gaacaaaaaa agccggcttc taagagccgg ccttttcttt tggaccttgg attttctatt
     2641 tatcctattt tttcagactc atactcctgt tgatggtgaa gcctaaagcc caattgctga
     2701 attttaagct actgctgttc ctggccaatt ggtcaatatt ggaagagttc gttgtattac
     2761 caatatagaa ctggaacagg tgaccaccgg tattaaattc catacctaaa gaaagaaggt
     2821 caggcttgta attaataata ttgccgttct tatccaacag gttttcatac atgttgagct
     2881 gacgggcata ttcaaaagtg atattcttgt tagcagtcag tttatatttt ccgcctaatc
     2941 ctaatgaacc aacataattt ttgttgttag gtccataagg aacaatatta aaatggatca
     3001 gcgtaggcat cagctgaagt gataatttat cagaaaactt acgggcaatc agcaactggt
     3061 gcataaagga gaacttatcc caggaagatg caacagcagg atcttttttt gcattcacat
     3121 taaacatgga ataccaggcc actgataccg gaatattctt agccccggtt tgctggcgca
     3181 gcaatttaaa cttggcccaa ccttcaaacc ggtttctgcc ggctccggcc aaacctaagt
     3241 tcatccaggt agtaggagaa taatcaaatg acaaatacgt ataggcaaga ccggtattta
     3301 aacccagtaa tcctgccagg ttatctcctg tttctgaact cccgccttcg ggccataacg
     3361 ggctaaagtg gtgagagatc ataaactgca aactaccggg tgttacgatt tctgtgctct
     3421 gcatattgat gatccgggta gagttaaacg tagcccgggt gaatttttgt tttggagctt
     3481 cttttgcttc cggctccgtg gtctcctgcg ccattgcaac tgccccaaaa cctaatccaa
     3541 aaattatgag tgataatatc tttttcatcg taaaatattt tttgattctt aatcttttat
     3601 ttctccatta ccggttactg aggcatccct gctgtgatcc aggatttaaa tttattaacg
     3661 gtgcaggaag tcaggctcaa tgccccaccc gggtgaccag tactgccatt actgttcatc
     3721 atcgcttcca gtatagttct tttggatgtc atggaactat agctatccag tgcaattaca
     3781 ccaccgccac cgtggcagga cccgccttta caggtagtat tataaatggg cagaattgtc
     3841 gcagtaaatg tttccccacc ggtagtacaa ccaccgccgg tatcatctga atcataagga
     3901 ggaccctgct caatccattt tttaattatg aatttctggt taggtgtaaa atcaatagtg
     3961 cctaatccgg ggtgttttcc gccattaacc caaacgctga atgcatttac acgggccagg
     4021 atcacatcat agaagacagt gtctttgtga gagaactgtg catatggttc gtttcccagg
     4081 ttatgacaac cacagggacc agcagtaaca atgggtacca catcttttct gaaagaaacc
     4141 ttttctaccg gtacctgtaa aatatcttct ctattgcgat aacaggatga caacatatac
     4201 atcaccatgg tagttgccat tgctgctaat aatatttttg ttttcatgat cttatattat
     4261 ttatgataaa tgttagcgtt tggttattac gttcatagtt tttttatacc tgaagatccc
     4321 ttcattattc ttaccatatt tatatacatc aggtatattg ggatcagcaa aataccagcc
     4381 cttgataata gctatttcag aaggacttaa gttaccatcc gctcttgcca taccaccctg
     4441 taagctggta gtccatgcat tggtatggat attggccaca tggaaggtag agtcaaccct
     4501 tgaaaccatg gtactgcttg cattcaggta agtattacta acatagaatt ttttacccgt
     4561 aaccggatcg gtataacgta ctgttgtccc tgttctcagg cttttgggat accatatatc
     4621 cataataata tcatcataat cattcaatgg tccgcggaca ctctgaactg tagtaggtga
     4681 tccaaatgtt ttccagggtc tgaaacttgc attagcgttg gtatcagcat aaaacctttt
     4741 aacgctgtct ttataagcac cccatacttc tttcattttg gtttcattga ctagctggca
     4801 atatgtattg gaacttgcat tcgtaaccca tttgatggta tccgctgaag taataccggg
     4861 gatcaaaccg cttctggccc atacaccggt agtagtagcc tggttatgac agttaccgga
     4921 agtacatcct gttgtaataa gatgaattgc agttggcctg taatcggcca gatcaggatt
     4981 ttcttttgca ccattcttga tccagttgta gataatattc ttgtctcttg tcgtcatatt
     5041 ggttggtgta ccatcactaa atacataatt aaccgggggc attgctttat tgaagtcgtt
     5101 ggtagtaata tactcccaaa gtttgctgga ctcgggactg cccggagtaa tgtacttggc
     5161 aacttctaag taagtatcca gtttcggaga aatcggacct ccgtgacagt tagctgtacc
     5221 gcagtactgt tttaagatcc ccatcacacc tcttgtcttc acgatatcat ttacatccgg
     5281 tgctgttggg tcagatggag atatcactaa agaatcgtaa aaaggagaaa atattgtgct
     5341 gtccggtgtt tccagaaatg acctgtcaaa ctgttttgac agcgcaccat cttttctgca
     5401 ctgaaataca gaagcggtca gaaatacacc acaaaggaag aggactgtgg tttgaataaa
     5461 agaaactttt ttcataaaaa gctagtttaa cttattgaaa attgatactg ataagaactt
     5521 tctaaaaatg cattgtcaaa aatattcagc cccgatttac agtttcatga catgaaactt
     5581 aatgctatat gactttggtc acattgtaat ccgattgtca tcaggtatta aatcaatttt
     5641 atataagaga atatatctgt gacatttctc acttattacc ttgacgcctg tcatgagatt
     5701 gtcagtaagt catatataag ttgcaccatt atttctttaa atttaagaaa ataaaaattt
     5761 tcattatggg aaacaataaa aagcctgctg ataaatcccg gcgggatttc ttatcgatgt
     5821 tcatatcggc tgataaaaaa gcagcttctt ctgaaacggt gaaaatgctg acaccggacg
     5881 gaaaattagt gcagatcagt aaagcagttt tggatgagat tacaaaaaaa caaaaagcta
     5941 ctaataaaga aatctataac tggatgaaca atccatctaa agaaaacagt taataaatct
     6001 gatcactcct tattaataat aagacaatga aacaagaaga agtaaatgat aaaggccggc
     6061 gggatttttt aaagaccgca gccctggcta cggtggcagc caccgcctgt ggaagccttg
     6121 cctgctcctg taattctaaa aaagccccgg tcagtgataa ggtaaagcta ttatccccca
     6181 ccggagaaat agtagaaatt gactctgcat acctgaatca tgaggaacat atggccattg
     6241 tttccaaact ggaagcaaga gaaggcattg ccggtaaaaa attcgtgatg gtggtagacc
     6301 tcgccaaatg taaaaatgcc cggagctgtg tttctgcctg tcaaaaatgg cattaccgtc
     6361 ctaaagaaac ggagtggctg accgtgaaac tgctgcagga cagtgataaa gcagctcctt
     6421 actggtttcc caagcaatgt ttccattgcg ataacccacc ctgtgtgaaa gtttgcccgg
     6481 tcgatgcaac ttataaaaga caagacggaa ttgtgttaat tgacagtgac cgttgtattg
     6541 gctgtaaatt ctgtatggct gcctgccctt actccacccg ggtttttaac tgggacgaac
     6601 ctgaactatc ccttgatata aaaaaccttg cttactcacc cgaaacgggt attccggcaa
     6661 aagtgggcac cgtagaaaaa tgcgatttct gtcctgacag ggcaagggag ggtttgctgc
     6721 cgccatgtgt ggaagcctgc cccaatggtg tattctattt tggtgatgaa aatgaagata
     6781 cggtcagcaa tggtctcgaa actgtcagtt tcacccaatt gataaaagac cgttccggct
     6841 accgcttcat ggaagacctg ggtaccaaac cacgggtata ttacctgcca ccaaaagaca
     6901 gggagttccc ggtagaaaaa ggttaccagg atgctgactc agccatcaaa gaacgcttta
     6961 aagatttcaa agatattctg gaagcaaagg aataactgaa cccatttccc cactttatca
     7021 atgaataaat aaattatgga actcaataat tatcaacaag aagctttcga ataccaacgg
     7081 aagaatatcg aaaaatatgg taaaagagcc tggggctgga ccatctttta tgtgctttgt
     7141 atccttgtcg gtctatatgc cctctatctc cagattgcca aaggacacgg tgtgacgggc
     7201 atgagagata atgtggtgtg gggattattc atcgtcaact tcatattttt tatcggtctc
     7261 agctatgccg gggctatcat tgcagggctg ctccacttat caaaagtaac ctggggcaaa
     7321 ccgatcatcc ggcttgccca gttcatgaca ctggtttccg tcataatagg tccaatcttc
     7381 atcctcctat gtgtgggccg cttcgaccgc atgcaccatt tgtttattta tccgaggatc
     7441 caatccccac tgacctggga tgtgatggcc gtaattacct tttttgtagg cagtgtattg
     7501 tttttatacc tggccctgat caaagacttc gcagtatatc gtgatgcaaa actgaatatc
     7561 ccgaaatgga aacaaaaact atacaaggtc ctcgccttag gttatcgtgg tgctgccagt
     7621 cagaaaagac acctgatcat ttcacaaaac ctgatggcgg tcattatgat ccctttagcg
     7681 atcatggtgg cctctattct ttcctggatt tttgggatga cgctgagacc gggttggcat
     7741 agcaccatct tcggccctta ctttgtatta ggagccgttt attccggatg cgggctattg
     7801 atagtagcca tgtggtttta ccggaaatta tataagctgg attattactt taccgataaa
     7861 cattttacat acctcggcta tgtgatgctg atcctggctg ccggttatgg ttattttact
     7921 ttctctgaat attttaccga ctggtttgct tcagaaaaat ggaacagcga actgatccat
     7981 aaactattca gtacttctga atatggctgg tggacccttt ttgccaatgc agccggtatt
     8041 gctttaccaa ttgccatcgt ggctattccg gccacccgta aaccgggccc cattacgatt
     8101 gccgccttta ttatggtaat tgccatgtgg gtgaaacgtt acctgatcat tgtacctaca
     8161 ctggaaacaa cattgttgcc gatgcaggat acccggacag aatatgtaaa atacgcagcc
     8221 acctggcccg aatgggcatt aacgattgca ggcatcgcct cgttcctgtt gttctttacc
     8281 gtgatgtcaa aatttgtaac ggtggttcct gtatcaggac tggaagatac acataccgtt
     8341 cacgaacaac acctgcttca ccctgaacct actttagaaa atacttcaaa ggcataacct
     8401 gctaaaatga tattatatga aacaagtaaa gtttaaaata acaagcacag gattttgcag
     8461 gcagttatta ttacttgcag gaatggcgct gtttatgctt ttcacgctgc ctgtatgggc
     8521 acaaaccgac agcacagttc agcaagaaac taccgaagcg ccggcaacag ctgaagagga
     8581 agaagaactg gtttcacctt ccattgaatt tatctgcatt cagaaaggtg ataagtctgt
     8641 tgatctgaaa gcagctgtta aggcaaaagt gcagggaaag atgagaaaat tgtatgggat
     8701 caaaacaaca ttttatctca ttacagattc cgcagaagcg gaactgggat cagtaatttc
     8761 tgacaggaac ggtgttgccc tttttaatgt aaaaccagaa caggttgttg ccaacaaaga
     8821 aggcaatctt cattttaaag ctgtactggg tgaagttaaa aaaatggaaa gcaccgaaga
     8881 ggaagtgaca gtaaaaagag ccctgttgag tatcactccc gtaaaagaag actccctgta
     8941 ttctgttcag gttaaattaa ttgatctttc tacaggagaa gaaacaccag ttccggaaac
     9001 tgcattgggg gtttttgtaa aacggtcctt taatccgatg aaaatagggg agggtactac
     9061 tgatgaaaac ggagaagcca gtgtggaaat acccaataat cttcccggtg acgcaaaggg
     9121 aaacattacc ctgttggtta agctggatga aagcgaaaca tatggctacc tggaagctgc
     9181 agcggtggaa aaatggggta tcccggtttc agataaaatc gaggaccagc caagatcatt
     9241 atggagttca cacccacctt tgtggatgtt gattacattc attatcttga tggccgctgt
     9301 atgggggcac tatatagtga tagtgtatga actgttccgg ctccggaaag aacagccaaa
     9361 tacagctgat aatgcaacta attcataact taagcaatct atttataact taaaacacat
     9421 gcacatgaaa acaaaagctc tcttattgat cgttagtata atggcctttg gcttatttgc
     9481 ttttattccc cgtacgatgg tacagccaca accatggcct gttcccgatg cttacaaaaa
     9541 caaggtaaat ccccttaaag gcgatgcaga gtctctggtt aacggtaaag cgctgtataa
     9601 ccagcattgt aaatcctgtc atggaaccaa aggaaaaggt gacggaccaa aagcttccca
     9661 gctggatact gattgtggtg attttaccaa atcaactttt cattcacaaa cagacgggtc
     9721 tttgttctat aaaaccttcg aaggacgtaa agacatgccg gcttacaaga aaaaaattcc
     9781 tgatcaaaat gatatctggg ctatcgtgaa ttatatgaga accttcaaat aatataaaca
     9841 gttgatcaat aaaaaaagcc atccgttcta aagcggatgg cttttttata tctctgattt
     9901 gtaataatta tttcccctga ccaggttcca gtaactggaa gaattgatcc agttgtggta
     9961 atatcacaat ccttgttctg cggttagctg ctttacctgc tttggttccg ttatcatcaa
    10021 cagggcggta ttcactgcga cctgcggctg ccattttcgc cgggtcaagg ccatattgct
    10081 tttgcaatat tctcaccacg gaagtagctc ttttcacact gagatcccaa ttgtccgtta
    10141 acacggatcc acggtaagga acattatctg tatgaccctc gaccataaat tcaatatccg
    10201 gttgattctt taacacctgg gccactttcc ccaatactcc cttcgccttt tctgttactt
    10261 catagctgcc gcttttgaac aggagtttat cagaaatgtc gatgtaaaca acacccttat
    10321 ctactttgat attgatatcc tggtcatcca tgttgccaat cgcccctttc aggttcatca
    10381 ccagtgccat attcagtgag tctttatgcg ccatttgttg ctgcagggtt tgaatataat
    10441 tatccttggc acctatattt tccattgatt tttttatact ttctgcttgt gtgctggtaa
    10501 tcactgaaag atcttccagc tgctttaaag cctggttatt attcgctttt aaaaaatcca
    10561 cctgtccgtt cagatcactt atccgggtat tctgcagggc tatttctctt tccagtgctg
    10621 ctttctccct ggccagtgta gctttttctg tactgcattc tgataaatca ctttccatgg
    10681 ttttgtaccg ggtttccagt tttgcataat cagcttttgc tgttttaaat tttttactgc
    10741 tgacacagga aaataataag agtggtgcgg ctgctgccaa aacaaattga tgtagtttca
    10801 taaagattaa tttgaggtaa ttaataaatg ataccgtagt agttttgaat tgtttgtatc
    10861 atttgcctta acggcagcaa agataaaagt acttgttacg gaattgattg atactttgtt
    10921 aattattaat atggataata gtgagaacag tctgacagac catcgaaaaa actaataaaa
    10981 ctcatatatt gccatgatat taaacattaa taaaaaccac cgtaacattc atttttacac
    11041 tactgaaata aattcttcat ttcagtacta cgattgcctg cctttttata aattcctttt
    11101 aaattattag aatctatgtc acaacaatcc aacttttttg ccatggagac tgctcctgcc
    11161 agagcagaac tggaaacatg ggtaaaacaa attgcttcct tctgcgaagc cagtaatgta
    11221 cactggtgcg atggatcaaa agaagaatat gacagccttt gccagctgct tgttacaaaa
    11281 ggaactttta tcaggctcaa ccctgaaaaa aggccgaata gttttgcctg tttcagcgat
    11341 ccaagcgatg tggccagagt ggaagacaaa acatttatct gtagcctgcg caaagaagat
    11401 gcaggaccta ccaataactg gatggaacct gtacagatga aaaagaaact acaggacctg
    11461 ctgcaggggg ccatgaaagg ccgcacctta tatgtaatcc ctttctgcat gggaccttta
    11521 ggttctccct tagcaagata tggtgtacag atcaccgact ctgaatatgt ggtagtgaat
    11581 atgcggatca tgacccgcat gggacgagaa gtgcttcctt acctgcaaaa gaaaagttat
    11641 gtaaaatgtc tgcatagtgt gggggcgccc ctggaaggca accagaaaga cagcagctgg
    11701 ccctgcaacc ctgatgtaaa atacatttct catttcccgg aagaacgttt gatcatctct
    11761 tacggaagtg gctatggcgg taatgcattg atgggtaaaa aatgttttgc gcttcgcatt
    11821 gcctctgtga tgggaagaga agaaggatgg cttgccgaac acatgcttat catgggagtg
    11881 gaatctccgg ataaacaaaa gacctatgta gcagctgcat ttccaagtgc ctgtggtaaa
    11941 accaattttg ccatgcttat tccacccaaa gaatttgacg gatggaaagt aactaccgtg
    12001 ggagatgata ttgcctggat acataaagca agtgatggtg gcttatatgc tatcaacccg
    12061 gaggcgggtt attttggtgt agcgccgggt accaactaca agaccaaccc taatgctatg
    12121 gaaagtatta aggccaatac catctttacc aatgtagcga tcactcctga taaagatgtg
    12181 tggtgggaag gattgaccaa agaaccaccg gcaggactta ccgattggca tggccagcca
    12241 tgggatccaa acagtggtaa accggctgca catcccaatt caagatttac tgcaccggct
    12301 tatcaaaatc cagctattga caaagattgg gataacccca atggtgtgcc tattgaaggt
    12361 tttatctttg gtggaagaag aagcaccggt gttccattag tatatcaatc tttcaactgg
    12421 aattttggtg tgtacctggc aacaactatg ggcagcgaaa tgaccgctgc tgcttttggt
    12481 gaaattggta aagttcgccg tgacccgttt gccatgttgc cctttatggg ttaccatatc
    12541 ggcgattatg taaaccattg gctgcagttt ggccgtaatc tccccaatgc cccccggatc
    12601 tttggtgtga actggttccg caaagatgaa aacggacagt ttcaatggcc gggtttcggt
    12661 gaaaatatcc gtgtactgaa atggatcgta gaacgtatcc gtggtaacgc ctcctctgtg
    12721 gaaagtccga ttggatggat gccccggtac gaggatatag actggaccgg tttggaaagt
    12781 tttaccaaag aagattttgc gaagatcatg acggtagacc gtgaaccggg taaacaggaa
    12841 ttattatctc acgaagagtt gtttgaacgt atgtatgata aactgcctaa agaatttttc
    12901 tttatgcggg aattacttct ctctgcctta tggcgttctc cggaacattg gggattacag
    12961 ccggagagaa actgacctct gccgattatt gcttgccctt ttaacaatca taaatagtgc
    13021 agcgggtatc cgcctgctgc ctgtttatga cgaatgatgt acattgtact gaatcgtttt
    13081 ttatgcctga atggtttcat gatcttttta caaaaacttc catagcccaa tcggtcatca
    13141 tctatgggct ggtgatcgct attggcatct ggatggggcg cattaaaatt gcgggaatat
    13201 cactgggcat cacctgggtg ctgtttgcag gattgatctt ttcttatttc ggtgtggaaa
    13261 tagattccgg tactgaacat tttatgaagg aatttggact tattttattt gtttactcta
    13321 tcggattgca ggttggaccc ggcttctggg cttcattgaa gaaaaatgca ttggtgaata
    13381 atctgcttgc ctttgccatt gtagctaccg gagttatcat caccgtcatc ctttatactg
    13441 tcagcggcaa cagcattgct gtaatggccg gcgtaatgag tggtgcagtg accaacaccc
    13501 ccggtctcgc tgctgcacag gctgctgtaa gggacctaca tgttacggtg gctgataaat
    13561 cactcatcac cctggcatat gctgttacgt atccgttagg cgtgtttggc atcattggct
    13621 ctttactgtt attgaaaaaa gtattcaaca ttaaaatcga aaaagaacag gagtttcacc
    13681 gcaagctggg tgtgctgcgg gccaaccggc ctgtttctgt acatctgaat ttagaaaaca
    13741 aacagttggt gggacaaccc ctgcgtaaat tgtttgacct gttaaaagag ccagttgtag
    13801 tatccagaat gtatcacaaa ggagaaatta ttacgccaac accagacctg atcctttctg
    13861 aaaatgatgt attgctggta gtgtcttcca aacaggaaat ggaacaattg aaattattga
    13921 ttggccgacc cagtaccatg aacctgaaaa cggcaaccga cagcgatctg atctcaagaa
    13981 tgatcgttgt aacaagatct gctgtcacgc ataaaagact gggtgatatc ccggagctcc
    14041 atcaacagga attcaccctc accaggttaa gccgtgcagg tattgaaatg gtaccacatg
    14101 gtgatatttt tttacagttg ggcgatacaa ttaaagtagt gggcaccgaa gaaggagtga
    14161 aagtggttac acaggcgctg ggtaattcat taaaaaaatt agaagtgcct gacctggccc
    14221 ccatctttat gggtattgta ttgggggtta tccttggcag tatccctttt cattttccta
    14281 atatgccggt agctgtaaaa attggtatgg ccggaggacc tttgattatt gccttaacat
    14341 taagccgttt tggaaatctt ttatacctca acaattatac aaccaacagc gccaacctta
    14401 tgatccgtga actgggtatc agtttgtttt tagccagtgt aggattaagc agtggaaaaa
    14461 acttatcggt tgcatttgct gacggtcgtg gctggctatg gatagggatg ggtgttatca
    14521 ttactgttgt tcccttatta ctaataggat ttattgcccg caaatatttt aagaaaactt
    14581 attttgaagt atgcggcttg ctggcaggcg cctccaccga tcccccggca ttggcatttg
    14641 ctactaaact ggccggcagt gatattccat cggtcactta cgctactgtg tatccgttga
    14701 caatgatatt acggatagta gcagcacagt tattgattct gctcttgctg taacttactt
    14761 cacagcattc ttcaacgctt ccactatttt cttttcgcta ccgatgaaaa ttttatcatt
    14821 aattatcgca accggtcttt tgagaaaagt gtattcatca aggatatact tccggtaatc
    14881 tttttccacc agctttttat ccttcagtcc aagttctttg tatttcatgg cccggcggct
    14941 gaagagggat tcatagctac cggccatttt tttcatttcg tccagctggg ccggtgttat
    15001 cttttcaaat tttatatcct gcaggacaaa ccctttctga tccgctttta tttctttaat
    15061 aattgcctgg caggtggtgc agtttccaag atgatagatt tttttcatat tgttactacg
    15121 ttactgatta tcgtttggtt cactcagccg taaaatgctt tactttgtag ccttaattat
    15181 tgttatgggc aaagatgaag tttttagaga cgaaatagaa aaagccagcg attttaagtt
    15241 tggcgccaac gtagcaaaag tctttgatga tatggtgaac cgttcggttc ccttctatgg
    15301 agaaatacaa cggatgatgg ccgaactggc tgctgatcat gccagggaag aaacatttgt
    15361 atatgatctt ggctgctcca ccggtaccac catgattggc atggatacga tggtaaacca
    15421 gcaaataaaa tttattggag tagacgactc gcaggaaatg ctggataaat gcaaatcaaa
    15481 gttgatggaa ctgggtttct cccgtcctta cgaattgcgt tgtgccgacc tcgggcaagg
    15541 agtaaaaatt gaaaatgctt ctgtagtcgt attatgtctt acacttcagt ttgtccgtcc
    15601 cgtttaccgg gaacgattac tgcaggatat ttaccagggc ctgaacccag gtggtgtttt
    15661 gatactggta gaaaaaatat tagcagaaga aagcaatttc aaccgtgatt ttatcaacta
    15721 ctattacaac tataaacgcc gtaataatta cagtgaaatg gaaatctctc aaaaaagaga
    15781 agcactggaa aatgtattag tgccttataa actgagcgaa aacatatcat tactgcggga
    15841 caaaggattt gcacattgtg aagttttttt caagtggtat aatttcgcag gccttatcgc
    15901 tgttaaaaaa taattggcat ggttagtatt ggaaatttct tctttaagta ccgcaactgg
    15961 atattcattc ttttttacgg cgctttattc atcccttcat ggcctttatt ttctaaagag
    16021 caattggggg aaagctatta tatatggccc atcagcattg gtttaatcat tacctgtttg
    16081 ggtcagctga tccgcggact gaccatcggg ctggcttata ttgtccgggg cggtaaagaa
    16141 gggaaaccat atgctgaagg attggttaca gaaggcatct ttacacattg ccgcaatccg
    16201 ttgtatgttg gaaacatcct gatgctgctt ggtgtgggta ttttggccaa ttcattattc
    16261 tatgttggaa taatgattcc cattttctta tttatctacc aggctattgt attagccgaa
    16321 gagaatttcc tccgcggaaa atttggtatt ggatttgacg aatactgtaa aaatgttaat
    16381 cgttggtttc ccaacctgaa agggatagga aaaacattca gtagtatgga gttcaactgg
    16441 aaaagatgga tattgaaaga acatactaca caattcatct ggctgatagg tatcacttta
    16501 ttattattgt tgaattatcc ggaactgacc gggcatgatg aaaagttccg caacctgctg
    16561 ctgggtatag ttctgggtgc tttgctgctt atttacattc ttatccgcta cctgaaaaag
    16621 acagggaaat ttcaggagta gctggaaagt atgtttcccg gttggctatt gtccggcggg
    16681 cttacggact tccgtcctcc agcctattcc ttcccgcctt cgtaaacttc caccgggatt
    16741 tgttgttatt tttgcatata aaattgtgag ttatgatcaa aacaggcaac cctatcatca
    16801 gcatttatac ggaaatgacg cctaatccgg aaacgatgaa gtttgtggct aacaagctgt
    16861 tataccccgg taaaagcatt gatttccctg atgcagagag tgcaaaacct tcaccgctcg
    16921 caacggagtt atttggcttt ccttttatca aagccgtttt cattgccagc aattttgtaa
    16981 ccctgacaaa gacaactgaa acagactgga atgatgccat tccttctatc cgtcaattcc
    17041 tgaaagatta tttagaagaa ggaaaggcca taatcaacga agaagaactg gctgcggtaa
    17101 aacaaaccag cagcaatgaa gtagctgctg atgatgacga tgtagtaaaa agaatcaaag
    17161 aactacttga taattatgtt cgcccggcag tagaaatgga tggtggcgct atacaattca
    17221 aaagctatga agccggtaca gtgagccttg ccatgcaagg aagctgcagt ggttgtccct
    17281 cttctatgat cacattgaaa gccggtatcg aaggcatgat gaaaagaatg attccggaag
    17341 taaaagaagt agtggcggaa gcagaataac taattattac agcggttaac aatttgaaga
    17401 atattgctat caccccgggc ttgacccggg gtctttttta aatgttcact gttctgtttt
    17461 ttttcagatc acattaaaag ccggtaataa aggcgtaatg aaaagaatga ctccggaagt
    17521 aaaagaagta gtggcggaag cataataact actgattaca tcggttaaca atttgaaaaa
    17581 tattattgtc accccgggct tgacccgggg tctttttaaa cgttcactgt tctgtttttt
    17641 tccagatcac ctttctattc ttcaaatacg ctatcacaaa atcaaaacaa tctttcctgt
    17701 ttccaatcag ttcaggcgga aatacccctt tctccgtaaa taatttttta gtgataagat
    17761 taacagcagc attgcaggta taacctgtag ttctggccat cgaagagatt ttagttaccg
    17821 gatcataggt atcatacagg tcatatatca ctgttttatc ttctcctttt ataaccactt
    17881 tcattacagt aaactcttct tcatccgggc cgggcttcca ttcattgacc aatagtgcag
    17941 aagtaaaatc tagcggcaca accgaaacac cattcaccct gataggtgtt atatcaaaaa
    18001 aaccagcctg ttgcaatgca atgatgaggt caatatgtcc cgggtaacgt aatgtctttt
    18061 ctttcatatc cgggatatga cccatcgtat gtatcaatga acgtaacccg tcggtattaa
    18121 aagcttccag ttcacccact ttatcaaaga gcatcaactc cctgtcgctc aatgccggct
    18181 ttgtaacaat gctgccttgt tccaccagcc ttgccggacg gatatattcc tgtatcacat
    18241 ccaccggtga gaaaggtgct ttgtattcaa atgggggaat tctttcttta ggtaaaccgc
    18301 caacataaca ttcaaacgct ttcactttca tttcggcatt ataacgaccg atgatgaaat
    18361 tactcatccc tggtgccaca ccacaatcgg ttatcactgt cactgccttt tcttttgcca
    18421 gcgcatccag ctgcatggct tcttctggga aaaatgaaat atccaccacg ttcttcccgg
    18481 cattgatcac cgcctccagt gttttataac ccataaaacc cggtacagct gttaccacca
    18541 aatcgaaagg agataaccag tttttgtatg catcaaattg agacagatcg gccatcactg
    18601 tctgaacgga agtatttctt tttattaata cctccaggtt tgcagcactt acatcaaagg
    18661 aagtaacagt ataatcgttg gctaaatcca gtgcaatggc ccggcctacc ataccggctc
    18721 ctaaaacagc aatcgtcata tattcatcgt ttggaacaaa ggtagcggaa ggaaaaagat
    18781 ccttattatg agaactgaag cagccaatat aacggtgcaa cgctataaac gtccaactaa
    18841 accattgata ttaaatcctg accgaaaatt aactgaacaa gtactccaca tactccctcg
    18901 tcagtgtctt cacaaaggaa gtatcatcag aaatgatgtc tttagccaat gctcttttct
    18961 tttcctgcag ttgcagtatt ttatcttcga tagtgtcttt acagatcatg cggtaagcaa
    19021 agatattatt ggtttgcccg atacggtggg tacggtcaat agagcctttt gttctactgc
    19081 agggttccgc caggggtcta caatatttac atagttggca gccatagtgt aatcattgct
    19141 gcccgcagtt ttccatagtg agtttggatt tattttctga atcactgttt attgtaccct
    19201 aaagtcatat ccgattcttt gtagcattgg gtcattgggg tcaacaccta atgtatattg
    19261 atgtaggtgt aaatgtttat tagggttagt tactgtcata acaaactccg catttgtaca
    19321 gtttttacct cttattccgt tgctaatgag gtctgacacc ccaaagccat aaatcccctt
    19381 ttttctacaa attattgctc ctttaaagtg atagttttgg gatgtttcaa aaaataaagt
    19441 gttaatgcct gaattttgat tacctgttct ttcttgaaaa tttccaataa ttgaagcgaa
    19501 ggcaaagcta tctttacccg gaagcggttg tcttgtaatt gtatctaaat aatagattct
    19561 aatatctccg ccaatccctg ttgaattgct atagtcaacg atagttgatg gattaagaag
    19621 gcttgttagt gtttttggaa aaatactttc aaaaaaaata gtatctccta tattataaat
    19681 ttctctttct ggggagtagt tacaagttac agcaaaactg taggtagcat tttggcatgg
    19741 cgtagtgctg tttttattac agcctgatga aagcaaaaac tgggctgccg taatcagcaa
    19801 cccagtttta agtatgttaa tagttgtacg cattgactaa agcatttact tgaacttgtt
    19861 ggttattccc gttttgctgc aatagcctat ccctaaaagc tggaattgtt cttacatcag
    19921 attgcagggc attaaaacat tgctgtattg taaagccagt aacattatca aatagtgggc
    19981 ctccaaaaga cgcatcgttt ctggggtcaa ataagtcata acacagacct tgcggtatcc
    20041 atcggtgtgc atcagcagcc cgattggggt caaaattttc aatagatgca aagttggcgt
    20101 ttaagccagc taagctgaat gtgaggtatc caccgtagtt tgcaagtgct gaaccatcat
    20161 ttatataaat tccattctga cctaatgaaa ttgtttccct tgccggatta gtatagtgcc
    20221 tttcactaaa ccatttctca caatggttac cccacatttc acccacggca acgattcctg
    20281 cattggtttc tgttccatcg ccatatgggt ctccaccaaa ggtttcagct aactcattag
    20341 aaatccttgt gcgatagacc tgccaattat cacagcctgc ctgtgcaaag tgggaggtat
    20401 gacctaattc atgatagaca attgccttaa cagtggaaga tgtgagtaag caattataat
    20461 cagcgactgt ggctgcataa ccaataatga catccgtttc attttttagt acttccacta
    20521 aatttacaat cggtacagat aataataagg gctgagctat aaaatattga acaaaccctt
    20581 gcagtaaatt cagttcgctg ttcaacacat ggcatttatt ccacattggg gcagcacctg
    20641 cacctcgctg gaagccccag tttgtaacaa tgatatttaa tttagtgggg tgatttagta
    20701 taccaacctc accagcatat tgtccatatt ccaaaacact gttgtgtgtg gtagtggcta
    20761 cccaatacgg aagttctttg tcatgtgcat tggttgggtt tggtttttca aataagtaag
    20821 atatgttagc cattgctatt tggtcaaaca cccccaatct tttctttacg ggaaaagcaa
    20881 tttgccaaag ccttatgccc cttaccttag cgactttggc attgttattt tctgttttaa
    20941 gaatgacttt tactttattc ctaaacctct ttgttgccaa aaactgccct tggtcatttg
    21001 tatatccagt ccaaattttg aaccaacgct tacaaacaat ccttgcttgt cttactggca
    21061 catttacaat aggagtagtt accgtattgc aggttctttg gtcttgtacc tgaatggtgc
    21121 ctcttgggat ttgagggttg taagtacctc caccgcctcc cccaccacct ccgcatcctg
    21181 tagggtcgca gccaggagca caggggtcta tttcacatgg tacgggtaaa cgatttggta
    21241 agattaatgt gtctgatggc tcatccactc tggtaatata tctgtctgct acaattacag
    21301 tatcgtcata aattgcacca cctgctaaac tttcagccat tccttccaat aacaagtcat
    21361 cattggcagg aatatgtaat tgttgtatga cttcatattg aataccggcg acaggtgtat
    21421 aatttgcagg aacagcagaa tacagccatc caatatcttc tgttccgaga gttgggtctt
    21481 gatagtagtc tccatcctgc aatactttgt agtccatcgg gtaatcctgt agctcaaggt
    21541 ttggctgatt taaaagtgtt gccagttggt cttgtgtagc aggtttatat cggatgtata
    21601 aatgcgtaac cggcacaggt gcggtgctgc catataattg tagatatgcc tgctgcatgt
    21661 tggcaatggt gtatgggttg ggtacttgtt gaccaagtat aatcgggtcg tcgccattat
    21721 tagggtcagg cgatgtgcca ttttcaataa gatttgatgt tggggcgttg gttggatctg
    21781 ctacacctgg aactggtagt tctttaaaaa aattgttagg aacttttaaa tttttatagt
    21841 caaactgtgt tttgtcaagt tgtagtgttt cggtgttggt gtattgcttt ttgcaactgt
    21901 tgattaatgc agtagccagg ataaggataa ctattcggtt gatgaatttc atataggttg
    21961 atttaaagtt acagaaaata atattttcgt tacataagta tagttgtcag ggaagatgtc
    22021 ttgtccgttg tataaaagtt tgttttatgg taagaaagaa tactataacc gttttttgtg
    22081 ttcttactaa gaagtacgac gaatagggcg gcatggaata aaagaggcct atatatagaa
    22141 cctgtgaaaa ggtaaagtcc tttttccaaa cctactatac cactaactgg taattcttct
    22201 ggcttaattg ttgataagtc ggagatatct agctgaaaag atactccaca tcctccctcg
    22261 tcagtgtctt cacaaaggaa gtatcatcag aaatgatgtc tttagccaaa gctcttttct
    22321 tttcctgcag ttgcagtatt ttatcttcga tcgtgtcttt acagatcata cggtaggcaa
    22381 agatgttttt ggtttgcccg atacggtggg tacggtcaat agcctgttgt tctactgccg
    22441 ggttccacca gggatcaaca atgtatacat aatcagcagc ggtcaggttc aaccccacac
    22501 caccggcttt taaagaaatc aggaaaactc ttacttcctc attattctgg aaactttgaa
    22561 tagctttttc cctgtcaggt gcggaagtgc tgccatcaaa atattcatat ttaataccca
    22621 gctcttccag cttggctctt atcaatgcca gcatacccag gaactgtgaa aagatcaatg
    22681 ccttatggtt gcccatatct tcacttagct ctcttgccag ttcgtccagc ttgatagagt
    22741 gattgtcaaa tttttcctgt tcgttcagta tcgccgggga gtcacatatc tggcgcagct
    22801 tcatcaggcc ctgcaaaatg gtcaactgtg atttttgtat cccctgcgtt tcaattgtac
    22861 ctaatatctg gttgcggaaa tcattgcggt atgcatcata gatattacgc tgttcatctt
    22921 ccatttcgca aaacagtatc atctcctgtt tttcaggcag atctttcgct acctgttcct
    22981 ttgttctgcg aagaataaac gggtataaaa ttttacgcag gtgatctttt cgatcctgtt
    23041 cgccaaactt atcaatcggt atagcaaact cctgccggaa aaattccatc gtacccagca
    23101 taccgggatt caggaaattc atttgggcaa aaatatcaaa cgtattattc tgcaatggcg
    23161 taccactcat acacaagcgg ttctttgcat tcagcaggca agcggctttg gttactttac
    23221 tggatggatt tttaatggct tgtgattcat ccagaataac atagtcaaaa tccacactca
    23281 tcagcagttt aatatcgctc cgtaatgtgc catacgttgt tatggtaaca tcgtggtcaa
    23341 tcagctcttc ttttgccctt gtccttactg caccatggtg aatacggtag gttagtgatg
    23401 gcgtaaattt ctttatttca ttttcccagt tatagatcaa tgttgtcggg caaactacta
    23461 atgcttttaa tttgccctgg tcatttttaa aatgctccag gaaggaaagg gcctgtaccg
    23521 ttttgcccaa acccatatca tcagccagta ttcctcccca cttgatatct ttcaggtaat
    23581 tgagccactg ataacccgct acctggtaag gacgaagtat tttttttaag ctatcagaag
    23641 ggtctatttc ttttatctta ttaaactccc tcaatttttc atatttctct tccagtttta
    23701 ctaccagttc ttcttcatcc cttgtctcat ataattcatc tactacgctt aaatgatagc
    23761 gggaaagttt aagcgaatcg gttttccctt cacccactct gaagagtaag gaatacttct
    23821 ttaaccattc ttccggaagg atacccaacg taccgtcatt caggtgaaca aattgttgct
    23881 tattggccaa agcccttttt acttcagcca cggttacctt ctgatcacca aatacaatat
    23941 ccactttggc atcaaaccaa tcggtattac tgctgatgaa gatctttgtt tgtggtttgg
    24001 cggtattgaa cctgaaattc tttaatgctt caaaaccaaa taccggtgtt ttatagtcct
    24061 tcatggcatc cacaaacaga aagaaccagt tattcttcaa cacatccgtt cctttcagtg
    24121 ccagtgttgc agaatcttcg ttatatacaa agttggaatg caggttttgt aatttctgaa
    24181 taaactcatt ttccttttcc ctgttccgat gcaccaccag tactttatct ccctgagata
    24241 cgattacttc atctctgtct ttcgcttttg tttcatagcc cttataagaa aaagaaggtt
    24301 gaaatacgag atagtctccc ttctccatca aaaacaactt cacttccggg ttgccctctt
    24361 ttatttcctg taccagtgat ttgtcaaaat caactttatg ttcttttgcc agcggcaaga
    24421 tgaacttatt caattggctt gtccattctt ctgctgctac attcatttta ccggttggta
    24481 aaaatttctc caccaggtga atcacctcat tatttttcca tagatacgcc tggttgttgt
    24541 acaaaaagaa taacgggctg gctgcctcgt tatctcccag atcaaattca agtgatccgg
    24601 cttttacccg gcattgaatt gtatagtgag aatttttctt tataataaaa tgcggctttg
    24661 cgtcttctgc ctgtagctgc agcggttcga gattattggt tttaaatgtt tttgattcag
    24721 gtaacaggaa aacaaagggg ttgccggctg attcggtaaa tattttcttc agtttaggta
    24781 ataagtattc agccatcagt tcctttgttt catctggcag gtcatcttct tcctggtgaa
    24841 tgatattttc ccaaataccg gagaaaggtg aattacggct gatgtactta ttaatctctg
    24901 aatcctgtag ttttcgcaac ataaccaata attccctgtc accttctggt aactggtcag
    24961 tatttacata cctgctgata tccaatttct ctactttacc ggcaaaagca tcgttgctgt
    25021 cattaggttc acccgtaact acatctactg taaaccgtgg gtaggttttc ttattaaagt
    25081 tgaaaacaat accgagccgt tgcgaagggt ttgcttcagc ctcttcggca acagcaggga
    25141 tttcatcagg ttccctttct ttttgcggaa ttagtaccgg ccgtgtaggc gcagccaccg
    25201 gagccaccct tttgatcttt acgtccagca ctctcaaaaa aggcttcccg tctttatacg
    25261 caaactcaaa ctttcctttt aagtcatcac tcaaagaata accatatgct tccagcaatt
    25321 tatttttttc cttatcccag ttgcggatac tgtcgaaata attagcgcca tatgcattca
    25381 gtagttgcag caggacaatc attttaggca aacacagcgg atgattctca tcttcataat
    25441 cacaactggt atcaaagttt ctttcctcat ttttgcggat aataactttg taggtttgac
    25501 cgtccagttt caccgaagct ttcactgttt cgttctctgc atattcaatt gtggctttat
    25561 ttgttcttaa aaaagtttcg gcatcgatca gtgtctccgg ggaacacaat aaacgcagtg
    25621 ttttcaaatc aattaatttc attttggcca ctgtgtgacg ctggtcatac ttcacgttgt
    25681 cagcctggag gtgaccccgg tcgatcattt cctgtaactg aaacaatgcc gctgcttcat
    25741 gacggcagat atcacccagg ttgtacggac aggcacaccg caatgatgtt gatttgggat
    25801 ctttaaattt ctgaatataa actttgtaat aagtagagta actgtcatcc tttacccgga
    25861 atacagcaga gccaaacagg tcatcgtatt ccaccagttc tacaaatcct attgcatgga
    25921 tctttttccc tctgcggatc acttcatcgg ttccatgggt ataaacatac ttgataaggt
    25981 gcggtaatgc caagcgatat acgattttag atatacgagg tacgaagtat ttttctccga
    26041 ccttcaaaaa aggttaatcg gcaaatgtaa aagaatgtac atcggatttc ctcgcaaaat
    26101 gcgataaata aaaaatattt tttattcagt aaatgtgccc ggaaaatgaa gtatcttaca
    26161 agatttttat ttcgaaatca gcatcccgtt actatataca ggtaacacat tggccacgtc
    26221 aggagtaagg cagaatttaa tatcttctat caatccaaat ctttcaacga gacgatgata
    26281 gtgcgtaagc ttcggagcaa attcaattaa tttattttta tgctttgtat acattgtctc
    26341 tgccatcagt gagctgtcac agtgaacagt aaattctttt gtaagacgat ttatcacggc
    26401 acctgcaaat aaggtgtctt ccaggttaaa acggtctttc cagccggcac aaccgagtac
    26461 cacattcttt ttctctgcta ataaaaaatc gcaaacagca gacaggttgg gaaatgaacc
    26521 ggagattatc atatctgcat tcctgtccaa tgccatttgc aggagtcttg ttccgttggt
    26581 agtagttaat accagcgttt tgttttcaat aaactccctg gtatattcaa gcggggagtt
    26641 gccatgttgt aaaccttctg cgatcattcc atctctttcg ccagctgcaa tgccaccgat
    26701 atttttactg atatcaatcg ctttcggaac tgaatccacc ggtatcacgc atttggctcc
    26761 gttatacaag gcggacgcaa t
//
LOCUS       DAORTI010000038        26291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1419_length_26291_cov_6.218059, whole genome shotgun sequence.
ACCESSION   DAORTI010000038 DAORTI010000000
VERSION     DAORTI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 26291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26291
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1419_length_26291_cov_6.218059"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="PLO70_06975"
     CDS             <1..2223
                     /locus_tag="PLO70_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQZ74241.1"
                     /translation="FLTEVITDRKNAAEELLKSQKQFQSLVENISGVYWVNNLDTQQS
                     LYISPSYNTIWGRSCEDHYKNPADFINSIHPEDKAVLSEMYSKIKVLRKAMPSYRIIR
                     PDGEIRWILAKVSVVADNKGQDIEYGYAEDITEQRRAEAELINSNARFQMVTKATSDL
                     VWDWDLQTDTLWWNDNYYTNLGYTKQNDIVNVNEWYDRIHPDDMARVKSKANKTFAGK
                     SSVWRDEYRYLKADGTYLHFLDRGFVLRNADGKAIRMIGSMVDMTPIYSVQKRVAESE
                     MRLRTILDTDPECIKLLDADARLLDINKAGLKMLEADTPEQVQGANLLPVVAEGQKEM
                     VEKLIKDAFNGKSGSLEFEMIALKGTHRWCEIYVVPFKNVEGAIVSALGVTRDITERK
                     KADLALRLSEEKYRTLIEQAADAIALFDASGKILEANTSASKLLGYTKDELSAMSLTD
                     ILLEEEIRDNPVQYDELSAGLSTVKLRRMRRKDGSVVVTEVRSQQLPDGRFLSVVRDM
                     TDRIKAEEELKLSYKAVRKLTAHLQDIREEERTNIAREIHDELGQQLTVLKMDVSWLN
                     KKFQGTDEKVNQRLKELLEMLDDTVKSVRRISSQLRPSLLDDLGLTAAMEWQLSEFEK
                     RAGIKTKFDAPEQEIDLSDATKTALFRIFQESLTNVARHSKAKKLSVILKRDDHNFIL
                     SIIDDGKGFDKNKIAEKRTLGILGMKERSSMIGGTYEINSTPGAGTSVIVSVPLNGNE
                     "
     gene            2243..2875
                     /locus_tag="PLO70_06980"
     CDS             2243..2875
                     /locus_tag="PLO70_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74242.1"
                     /translation="MLNRILIADDHAIVRRGMRQLLLEHYPFATIGEAANVEELISLV
                     INQQWDVVVCDMNMPGRSGLDALMQIKEISPKLPVLIMSMYPEDQYALRVLKSGAAGY
                     LGKETIHDNIVSAIELVVQGKRYITPAVAEKLAEAVNSDLPRVLHEGLSDREFDVFKL
                     LASGKSVSEIAAQLSLSSTTVSTYRSRIMEKMNMRSNAELARYALEHNLI"
     gene            2995..3387
                     /locus_tag="PLO70_06985"
     CDS             2995..3387
                     /locus_tag="PLO70_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ74243.1"
                     /translation="MKKELTILIVDDNKNFVDRMISLLEEVTTNKRISTASNYEEARR
                     LLSGEQQDIVLLDINMPGKNGIELLKFIMQKNNQSEVIMITNHADTYYKEQCTELGAK
                     YFLDKSNDFALVPGIVKEFFLKKYKNFK"
     gene            3403..5601
                     /locus_tag="PLO70_06990"
     CDS             3403..5601
                     /locus_tag="PLO70_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQZ74244.1"
                     /translation="MRAEWRTISNIIRVSDSPKDKFISLIAEDGTIMNANARMVRSLH
                     IKNPKSSSVNFFDLLHPLNKSDFKNGFRQSIEGGSPVAMEGYLKNGYYHPMKWQISPV
                     IKEGKPVVYLCIGHKLIDDDRLHKFNRLGENNYQLIVESLNAGILFQDSNGDIIAVNQ
                     KSAEIFNATLERLYQLKDVANLWNTAWDIKNENGDPVPFENTPFMKALQSGKTQTEVL
                     LIRLLDGQCRWLHFSSQPLFNENADMPFAVVSNITDLTYERTLIRELKDGKAILNSFI
                     NQTPNLVWVVDELSTLLFASKAFYKYFGLKAAESVNKNIIDLVPESVAKSLYEKHIQV
                     LETGMPAEFIEKVKWADGTDFIFHINIFIIEGISNKRILGGHAVNLADKYATEQKLRE
                     ANERLLLLTRATSDAIWEWDMQTGHIFRNDALMDMIGYHTDAPKGLSWWLRRIHPDDR
                     NRVADKVKDSTDNGRQSWDDEYRFKCADGTYKHMRDKGYIVYESGLPVKMIGSLQDIT
                     GLKELECQLMEEKLERQREISEMMIRAQEKERTRIGHELHDNVNQILSSTKLFIEMLK
                     PSGKEQKDFKNKSIEYISLALEEIRKLSKELVVPQLKDNSLIPAVKQIINDIHLSTPI
                     LIKFVHDHEADLLTAAKKVTLFRILQEQLKNIIKHSRAGNVYISLQRNGADIALVIKD
                     DGIGFDLSQTHRGIGLSNIYERVRFYNGTADIKTAPGEGCTLTIVIPMLQ"
     gene            complement(5659..6249)
                     /gene="hisIE"
                     /locus_tag="PLO70_06995"
     CDS             complement(5659..6249)
                     /gene="hisIE"
                     /locus_tag="PLO70_06995"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQZ74245.1"
                     /translation="MIPDFSKYSDGLVPTIIQDANTGKVLMLGFMNAEAFQRSNQEGK
                     VTFYSRSKQRLWTKGDTSGNFLFIKEIISDCDNDTLLIKVNPAGPVCHTGADTCFNED
                     NAGFTLKKLENIIADRKNRPSDSSYTSSLFGKGINKIAQKVGEEAVELVIESKDTNDV
                     LFLGEAADLLFHYLVLLQAKNHSLEDVVQILAKRHK"
     gene            complement(6412..7167)
                     /gene="hisF"
                     /locus_tag="PLO70_07000"
     CDS             complement(6412..7167)
                     /gene="hisF"
                     /locus_tag="PLO70_07000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQZ74246.1"
                     /translation="MLTKRIIPCLDIKDGRTVKGTNFIQLRDAGNPVELAVRYCEEGA
                     DELVFLDITATTEKRKTLAKLVKEIATHINIPFTVGGGINTSEDVSVLLENGADKISV
                     NTSAAKNPQLIDELAKNFGSQCVVVAIDAKLINHQWMVVTHGGRTPTEKLTIEWAKEV
                     TERGAGEILLTSMENDGTKNGFAIEITKKVATAVNIPVIASGGAGKMEHFTEVFKSAN
                     ADAALAASIFHFREIAIPDLKRYLYNQHIPVRI"
     gene            complement(7171..7887)
                     /gene="hisA"
                     /locus_tag="PLO70_07005"
     CDS             complement(7171..7887)
                     /gene="hisA"
                     /locus_tag="PLO70_07005"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQZ74247.1"
                     /translation="MTIIPAIDIIDGKCVRLTKGDYSQQTTYNEDPLEVAQQFEDAGL
                     QRLHLVDLDGAKAGEVKNWKILEKIAGKTNLLVDFSGGISTLKNVEIAFGSGAAYAAI
                     GSMAVKDEFTLSGWLMAFGADKFIIGADVKDEIIVIRGWTETTKITVFELIEKYKLKG
                     VKDFFCTDVNKDGLLQGPATDLYKKILNQHPSINLIASGGVTSLEELEDLRQAGCKGA
                     IIGKAIYENRIALSDLKRFF"
     gene            complement(7884..8480)
                     /gene="hisH"
                     /locus_tag="PLO70_07010"
     CDS             complement(7884..8480)
                     /gene="hisH"
                     /locus_tag="PLO70_07010"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQZ74248.1"
                     /translation="MNIVIVKYNAGNIQSVLFALERIGATATVTDDKELIQTADKVIF
                     PGVGEASTAMCYLKERELDKVIVGLQQPVLGICLGMQLLCEHSEENNTDCLGIFKNVM
                     VRKFNGVNTNVKVPQVGWNNIYNLKSELFSSVDENTYCYFVHSYFAEQNPFSIAETDY
                     IQPYSSALKKDKFYGVQFHPEKSSKAGEEILKNFLKIK"
     gene            complement(8480..9619)
                     /gene="hisB"
                     /locus_tag="PLO70_07015"
     CDS             complement(8480..9619)
                     /gene="hisB"
                     /locus_tag="PLO70_07015"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HQZ74249.1"
                     /translation="MRKILFIDRDGTLIKEAPPTYQIDTFEKLEFYPDMFTWVRKIAK
                     DLDFELVMVTNQDGLGTASYPEDTFWPIQNFVLKSLENEEIIFSNIHIDKTFAADDIP
                     TRKPGTGMLTQYLNNPEYDITGSFVIGDRITDIQLAKNLGCKAIWLNNDPLLGAEEIS
                     RTDQHFEEIIALETTSWKSIYEYLRLGQRVIQHERNTNETKIRILLNLDGTGVANIDT
                     GLNFFNHMLEQLCRHSGIDITITVKGDLHIDEHHSIEDTAIALGEAFALALGDKKGIG
                     RYGFALPMDDSLAQVAIDFGGRNWLVWHADFKREKIGDVPTEMFYHFFKSFSDAAKCN
                     LNIKVEGDNEHHKIESIFKALAKSIKMAVKRDVDNMQLPSTKGIL"
     gene            complement(9616..10668)
                     /gene="hisC"
                     /locus_tag="PLO70_07020"
     CDS             complement(9616..10668)
                     /gene="hisC"
                     /locus_tag="PLO70_07020"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQZ74250.1"
                     /translation="MEFNVDNLVRENIKKMTPYSSARHEFSGTATILLDANENSFGSP
                     LPENYNRYPDPLQLTLKEKISSIKGVPAQNIFLGNGSDEAIDLLFRIFCEPGKDNVII
                     FPPTYGMYEVCAEMNAVKVKKVPLTQDFQLNLGAIEEAIDANTKLIFVCSPNNPTGNS
                     INRDDIEVLLNNFDGLVIIDEAYINYAKQKTFIPELTEYANLVILQTLSKAWGLAGLR
                     LGMAFAGEPVINYFNKVKYPYNINTATQQLAIEALGNISSVNNWTKTTVEQKGLLQEE
                     LLKLPFTQYVYPSDANFILVKMRAARNIYEYLSGKGIIVRDRSKVILCDDSLRITIGT
                     PEENKQLIEALKSYTA"
     gene            complement(10671..11948)
                     /gene="hisD"
                     /locus_tag="PLO70_07025"
     CDS             complement(10671..11948)
                     /gene="hisD"
                     /locus_tag="PLO70_07025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQZ74251.1"
                     /translation="MKVYKYPDKGNWEEIMQRPASDYSSLEKSVKKILQKVKDKGDKA
                     IRKFSKEFDGASLKRIQVSEKEINTAASLLPEELKQAIQQAKKNIEQFHQSQKEEVKK
                     VETMPGVLCWREAVGIEKVGLYIPGGSAPLFSTVLMLAVPAQIAGCKEIILSTPPAKD
                     GSINPAILFAAQLCGITKIFKAGGVQAIAAMAYGTETIPKVFKIFGPGNQYVTLAKQI
                     IQQQGVAIDMPAGPSEVLVIADETAVPEFVAADLLSQAEHGTDSQVILLTTSAGIAEN
                     VATAINEQLKELPRKSIAEKALHNSKIVIVKNIAIAIELSNCYAPEHLILSCADAEQV
                     AQKVTTAGSVFIGNYSPESAGDYASGTNHTLPTNGYAAMYSGVSLDSFVKKITYQKLT
                     KEGLKNIAPAVMAMAGAEGLDAHKRAVAIRLKK"
     gene            complement(11948..12808)
                     /gene="hisG"
                     /locus_tag="PLO70_07030"
     CDS             complement(11948..12808)
                     /gene="hisG"
                     /locus_tag="PLO70_07030"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQZ74252.1"
                     /translation="MSETLKIAVQKSGRLYEGSMKLLKECGIEVSNGNNQLRVLSSNF
                     PVEVFFLRDDDIPEYVQDAVADIGFVGENVVREKNKIVDSIEKLGFGKCRLSIAIPRN
                     GQLQSISDLNGKKIATSYPVILTTFLNEKKIAASIHEISGSVEIAPRIGLADAICDLV
                     SSGSTLFTNELKEIETILFSEAVLIGNKALSTEKRIILESLLFRIRSVKKAKNNKYVL
                     LNAPNDKLDTICSLLPGMKSPTILPLAEPGWSSVHSVINESDFWDVIEQLKANGAQGI
                     LIIPIEKMII"
     gene            13017..14219
                     /locus_tag="PLO70_07035"
     CDS             13017..14219
                     /locus_tag="PLO70_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ74253.1"
                     /translation="MAKLTRTTNSGHWHRLGIFNPFAPGAETIDPLKPWKIISQNNIF
                     IVYLSILLFAFYAFTRHGLNWIALSTSLIASIPVGFAIAFFETQAMEFRRKHRRTGFW
                     YYLLYRTLRITFWFMVLFFIIMNIVLWLNINTYETIFPHSLHDFFATRKLFEFLGLSL
                     IISLVSNFLDELHRKLGRDAIFNLVFGKYHQVKQEDRIFLFIDLNHSTDIAEEMGEME
                     YSHFLQDYYYDISEPIARYMGRVYQYVGDEVVVSWPVKKGLRFALCIRCFFAIEKQIN
                     RYREQYLERYGRVPEFKASLHGGYVVVSEVGKYKSEIAYHGDVLNTTARMLGKCHDLN
                     ATFIISDWIMKNVIAPKYLKMESLGLFQLKGKQQEVELFSVYMSQKKQEEKKIGRKGF
                     FRRKPGVD"
     gene            complement(14216..14551)
                     /gene="ytxJ"
                     /locus_tag="PLO70_07040"
     CDS             complement(14216..14551)
                     /gene="ytxJ"
                     /locus_tag="PLO70_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HQZ74254.1"
                     /translation="MNWIDLTSENQLKDIIASSYDRPQIIFKHSTRCSVSSIALSRLK
                     KSGPLSNSDFFYLDLLSYRQLSNKIAELFRVPHESPQILLIKNGECIYEESHLGITAD
                     DLQEQLIVA"
     gene            14936..15703
                     /locus_tag="PLO70_07045"
     CDS             14936..15703
                     /locus_tag="PLO70_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQZ74255.1"
                     /translation="MLQLKKPLAFIDLETTGVNPGTDRIVEIAIIKILPDGARSVKRK
                     LINPEMPIPKNASDIHGISDEMVKDAPTFKQVAHELKQMLDGCDFAGYNSNRFDIPLL
                     MEEFLRAQVDFDMKNRKLLDVQNIFHKMEPRTLSAAYKFYCSKSLDGAHSAEVDASAT
                     HEILEAQLERYTELGNTVDSILKVIGEDQVVDFARRFVMENGTEVFNFGKFKGRPVAD
                     VLKAEPQYYDWMMKGDFPQHTKQKLTEIYTRTLLKKG"
     gene            15790..16593
                     /locus_tag="PLO70_07050"
     CDS             15790..16593
                     /locus_tag="PLO70_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQZ74256.1"
                     /translation="MEKLVVIPTYNEKENITSILHAIFNLHEDFHVLVIDDGSPDGTA
                     LIVKELQAKFPGQLFIEERKGKLGLGTAYIHGFKWAIAREYAFIFEMDADFSHNPNDL
                     PRLYNACKNEGADLAIGSRYVTGGGVVNWPQNRIALSKGGSLYTRMITWMPVKDPTAG
                     FMCYKKEVLETINLDKIEFVGYAFQIEMKFAAWKLGFKIKEVPIIFQDRTYGVSKMNK
                     GIIKEGVIGVLKLRWHSLFKNYRKRLKNTDAEISPLTGIRKNEMLAESE"
     gene            16636..17529
                     /locus_tag="PLO70_07055"
     CDS             16636..17529
                     /locus_tag="PLO70_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQZ74257.1"
                     /translation="MKKAFLQLHIAVFLAGFTGILGRLITLNEGMIVWYRLLFTAVTM
                     WILFGLMKKLQKIPFIDILKITGVGFIAAMHWVTFYGSIKYSNVSVALVCFSAISFFT
                     ALFEPLILKKKINWLELLLGLVTLSGIYIIFHFDTRFKTGIVIGLISAVLASLFPIYN
                     REFLKRINVETMLTWQQTGGFLVLSLLLPFYLQQFPIENFIPDRENIGWLLVLAWFCS
                     VIAFQLSANALKKLSAFTVNLTYNLEPVYGILLAFAVYNENQYLSKWFFVGFAIIAVA
                     LLIHVYLLFRVERKLTEHAAD"
     gene            17516..18373
                     /locus_tag="PLO70_07060"
     CDS             17516..18373
                     /locus_tag="PLO70_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQZ74258.1"
                     /translation="MQLTEYHQKIIDYYRDTENSYKDSWDLNNSLAIHYGYWDATVKT
                     FRRSLLRMNEIMMEAAAIKATDKVLDAGCGVGGSSIFIASLLGCKVTGITLSERQVQQ
                     ANENARRKGVEDLVNFSVMNFSATDFADASFDVVWGCESICYAEDKEQFIKEAFRLLR
                     PGGRLVVADGLVTDFENNENPDIRQWLDGWQVNYLESPERFQSFMLQAGFGNINFRNI
                     SRETAHSSRRLYRIYYLASLYLLWKKVNFSKPVTAIQKKNIKACKYQFKGMQKRLWQY
                     GLITGTKLR"
     gene            complement(18378..20819)
                     /locus_tag="PLO70_07065"
     CDS             complement(18378..20819)
                     /locus_tag="PLO70_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ74259.1"
                     /translation="MIKIFSLLIAITLFYQAQAQRQGSITGTVTDKNLLTPVGSVSVQ
                     VIGTSFGTTTDSSGKFRISGILPKSYTLQFTSVGYNEVTLFNIVITSGNEINLTVQLE
                     PSITRLEDVVIKSSRRTAVAASLETPLSVQRLTTEEIRSNPGGNFDISRVIQALPGVG
                     GTAGSVGGFRNDIIIRGGAPNENVYYLDGIEIPVINHFVTQGSAGGPTGILNVSFLED
                     VKLSSSAFDARYDNALSSVLQFRQRNGNPNSLQGNIRLSGTELAGTFEGPLSKNTTFL
                     ASARRSYLQLLFKAIDLPIRPNYWDFQYKVNHRIDRKTTLTLIGVGAIDDFSFAAPRK
                     ATPEKLYVLDATAPIHQWNYTIGANIRRQIKNGYWNLALSRNMFDNKIEKFDDNEEEN
                     ETKRRIGIFSQEIENKLRFDVNKTINGWKLAYGGITQYVKSNNNIFKRFRKELTDQNG
                     NIIQPEISVRYNSDISFWRMGAFVQASKRYLDDRLGISAGLRSDLNTFTEDGMNPIST
                     LSPRISASYVLADKWTLNASIGSYSKIPTYTILSFRDNSGVLMNKNVDYTRSIHYVLG
                     TEYLPNTTTRFTLEAFYKQYRNVPVSVKDGISLANMGGDFGVIGNEDVITNGKGKSYG
                     FELFAQQKLTKRFFGTLSYTFFISRFSGLNKKEIASAWDNRHLLSFIWGYKFNKNWEL
                     GLKFRYQGGAPYTPFDLTASQLNYLTYSIGILNYDQLNSEKLAAFHSSDLRIDKKWNF
                     KKTTLNLYLDVTNWYNARNPAFPQYTFKRNADNTAFLTTDGQPIRQDGSNAIPFILAN
                     DDASVIPTIGFIIEF"
     gene            20894..23863
                     /locus_tag="PLO70_07070"
     CDS             20894..23863
                     /locus_tag="PLO70_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQZ74260.1"
                     /translation="MRKSNFSLVLLLLLVTGLYAQKKPLDHSVYDSWQRIGERMISND
                     GKWVVYTIDPQEGDNELVIQSSDAKYKKTIARGYNPVITEDSRYAIFRIKPLFKDTRE
                     AKIKKKKPDDMPKDSFAIVELGQDSVWKVARVKGYKTPEKEAGWVAYHLEKAIEKKET
                     AAKKPESNGKKITDSLNSVIDSLHQVIQSIPKQKKKKRDDDEAFTDPGFFIAENHDAE
                     GDETPGTPTEAGTDLVLRNLNNGQEKIFNNVLEYYFSKKGGRLLIEQAKNPKDSLSKP
                     SVSLYDLSEAKAIVLSRGGNDFKNFVFSEDGSQVAYVAERDARPKELQKFYRVWYHKT
                     GLDTAILLADKNSVGMKLGMTISEFGNLSFSKSGNRLFFGTAPIQPPKDTSLVEMDLV
                     KLDIWNYKDDYLQTQQLFRLQRDLQTNYLAVYHLGKNELKQLGSEEIPIIFPTREGDG
                     DQFVGVTDFGKRIESQWAGNTKKDIYAINVNNGSQKLVKRDMTGVITPQYISPNGKYI
                     MWYDSKVRHYFVWDGNTTRNITAKIKFSLWDEEHDTPNDPSPYGVMGWHEGDSAVYVY
                     DRYDVWKCPLAEKSNSINITGVGRTGKTRIRYLQTDPESRYLKPYDDIFYRLFHEKDK
                     SEEIIQSSLKEWFSFEQAEAGAYMFGQIKKAKNASTLLYTIESYSRTPDLFADQLIPN
                     SIDEAIRLSYINQQQKDYNWGTAELYKWKAFNGKMSEGVLYKPENFNPNKKYPLIMYF
                     YETHANTLHSFIPPTPTGSRLNISFFVSRGYVVFSPDIRYTIGHPAKSCYDYVVSAAQ
                     DLAKQRWIDAKNIGIQGQSWGGIQVAQLITMTNMFKAAWAGAPVANMTSAYGGIRWES
                     GANRQFQYEKTQSRIGATLWEKPNLYIENSPLFHVPKIKTPLVIMHNDADGAVPWYQG
                     IELFTAMRRLGKQVWMLNYNGEAHNLRERKNQKDISIREQQFFDWLLKGEKPPKWITE
                     GVPAVKKGKDWGLELVN"
     gene            complement(23916..24632)
                     /locus_tag="PLO70_07075"
     CDS             complement(23916..24632)
                     /locus_tag="PLO70_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74261.1"
                     /translation="MKSLSETWFAEGRIDFELKKYTLLAYLQEVNKYFTENKLYPQLA
                     DIIFHYNNIVAFRENKKYLQEHFPKKLTGIQMEKLQVLYQQMIEDDELMQELEDIIQY
                     SAGKIKTTIHNGTEIYEFVEEKINIVPVGLIPLDTQEGYFFLSSGNVKSTRVYHYRLS
                     IFEKHDEKYRSIKTAFIDNKLRSISSTYEHMKSELIRQRKDLPNPAVYLIETELTFPI
                     DETLLPIAKRSLVKFISTAA"
     gene            24719..25240
                     /locus_tag="PLO70_07080"
     CDS             24719..25240
                     /locus_tag="PLO70_07080"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HQZ74262.1"
                     /translation="MRVLLLIISVTLFSCGNTIYIVRHAEKAPVEAGASQMMASDPPL
                     SDPGKLRAIALRDKLKGENIRFIFSTNTKRTIATAQPLNDQRSNTSIEIYSSKKDSMD
                     IFIEKLKTIRKGNILVVGHSNTVDDIVNRLSQRTEIAADLKDNEYDNLFILKRKGDSY
                     LYRNEKYGNKSPD"
     gene            complement(25237..25938)
                     /locus_tag="PLO70_07085"
     CDS             complement(25237..25938)
                     /locus_tag="PLO70_07085"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQZ74263.1"
                     /translation="MKYFRFLIFLFFIQPGAAQTGSWNIINTKLDLTEKWNLFGELQL
                     RSLRFYDDFHYYEIKGGASFAISKASTISAGFGIFDTYSPGGNFKSPMVNDEWRSWIQ
                     FNMTQYEKRLKLEHRYRAEQRWTSDGFRNRFRYRINSALPLNNKKIEAGTYYLTASNE
                     IFFTNRAPYFERNRVFLGAGYEFSPFFTLQAGYLHQFDYKISDETGKNFLQLSFLFEM
                     QWKKKTGEKVPGGMD"
     gene            complement(26000..>26291)
                     /locus_tag="PLO70_07090"
     CDS             complement(26000..>26291)
                     /locus_tag="PLO70_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQZ74264.1"
                     /translation="PDDTTGGTFTLTNVGTFGSIMGTPIINQPQVAIMAVGAIKKRPV
                     VIETPAGDSIAIRHMMYLSMSYDHRIVDGSLGATFLTAVAKELENFNPEREF"
BASE COUNT         7820 a         4929 c         5556 g         7986 t
ORIGIN      
        1 tttctgactg aagtgattac tgatcgaaaa aatgcagcgg aggaactcct taaatctcaa
       61 aagcaattcc aaagtttggt ggagaatatt tcgggagttt actgggtgaa taatcttgat
      121 acgcaacaat cgctctacat tagtccttct tataatacta tttggggaag aagctgtgaa
      181 gaccactata agaatccggc ggatttcatt aattcgatcc atccggagga taaagcagta
      241 ttatccgaaa tgtattccaa aataaaagta ttgagaaagg ctatgccctc ttaccggatt
      301 atcaggcctg atggagaaat aagatggata ttggcaaaag tatcagtagt ggcagataat
      361 aaaggacagg atattgaata tggttatgcg gaagacatta ctgaacaaag acgggctgaa
      421 gcagaactaa taaactccaa tgcccgtttt caaatggtta caaaagctac aagtgacctg
      481 gtgtgggatt gggatctgca aacagataca ttgtggtgga atgacaatta ttatactaat
      541 ctgggataca caaaacaaaa tgatattgtt aatgtcaatg aatggtatga tcgtattcat
      601 cccgatgata tggccagagt gaaaagcaaa gcaaataaaa cattcgcagg taagagttct
      661 gtatggagag atgagtatcg ttatttaaag gctgatggaa cctatttgca ttttcttgac
      721 cggggttttg ttttgcgcaa cgctgacgga aaagcgatca gaatgatcgg ttcgatggtt
      781 gatatgactc ctatctatag tgttcagaaa agagtagccg aaagtgaaat gaggctacgg
      841 acaatactgg atacagatcc cgaatgtatt aaactattgg atgcagatgc caggctgctg
      901 gatattaata aagccgggtt gaagatgctt gaggcagata caccagagca ggttcagggt
      961 gcgaacctcc tgcctgttgt ggcggaaggg caaaaagaaa tggtagaaaa gttaattaaa
     1021 gatgctttta atggaaaatc cggcagtttg gaatttgaaa tgatagcact aaaaggtaca
     1081 catagatggt gtgagatata tgttgttccc tttaagaatg tagagggtgc aatagtcagt
     1141 gcattgggag taacgagaga tataaccgaa agaaaaaaag cagatcttgc actacgatta
     1201 agtgaagaga aatatcgaac actaattgaa caggcagctg atgcgattgc actttttgat
     1261 gcaagcggaa agatacttga agcaaacaca tctgcatcaa agttgctggg ttatacaaag
     1321 gatgagttat ccgccatgag tctgacagat atacttttag aagaggagat aagagataat
     1381 cctgttcagt atgatgaact tagtgcaggc ctatcaacag ttaagttgcg taggatgaga
     1441 agaaaagatg gttcggtagt tgttacggaa gttagatcgc agcagttgcc cgatggccgc
     1501 ttcttatcag ttgtaaggga catgacagac cggatcaaag cagaggagga acttaagtta
     1561 tcttataaag cagtacgaaa actcactgca cacttacagg atatcaggga agaagagagg
     1621 acaaatatag caagagagat acatgatgaa ttaggacaac aattaactgt tctgaaaatg
     1681 gatgtatcct ggctcaataa gaaatttcag ggtactgatg aaaaagttaa ccaacgatta
     1741 aaggaattac ttgaaatgct ggatgacacc gtaaagtccg tccgacggat ctcttcacaa
     1801 ctcaggccaa gtttactgga tgatctgggc ctaacggcgg ccatggaatg gcaactgagt
     1861 gaatttgaaa aaagagctgg catcaaaaca aaatttgatg ctccggaaca agagattgac
     1921 ctttccgatg ccaccaaaac agctttattc agaatatttc aggaatctct tacaaatgta
     1981 gccaggcatt caaaagctaa aaaattatcg gtaatactta aaagggacga ccataatttt
     2041 attctgtcaa ttattgacga tggaaagggg tttgataaaa ataaaattgc ggaaaagaga
     2101 acacttggta ttttggggat gaaagaaaga agttctatga tcggggggac ttatgaaatt
     2161 aacagtactc ccggtgccgg aacatcagtt attgtttcag tacccttgaa cggaaatgaa
     2221 taaaatagat tatcagaaaa ttatgctgaa ccggatttta atagcagatg atcatgcaat
     2281 tgtacgcagg ggaatgcggc agttgttact ggaacattat ccctttgcta cgattggtga
     2341 ggcggccaat gtagaggagc ttatcagttt ggtcataaat cagcaatggg atgtagtggt
     2401 ctgtgatatg aatatgccgg gtcgaagcgg tcttgatgct ttaatgcaga taaaagaaat
     2461 atctcctaaa ctacctgttc ttattatgag tatgtacccg gaagatcagt atgcattacg
     2521 ggtattgaag tcaggtgctg cgggatacct tggcaaagaa acaattcatg acaatattgt
     2581 aagtgctatt gaattggtgg ttcaggggaa gcgatatata acaccggctg ttgcagaaaa
     2641 actagccgaa gcggtaaatt cagatttacc cagggtatta catgagggtc tttctgatag
     2701 ggaattcgat gtgtttaaat tattggcttc cggaaagtct gtatcagaaa tagctgccca
     2761 gctttcactc agctctacaa cagtcagcac ttatcgttcc cgtattatgg aaaagatgaa
     2821 tatgcgaagt aatgcagaac ttgccaggta tgcgcttgag cataacctga tctaagcctc
     2881 ccgaacattt gttcacctgt agttatttca ctacaatttt ttagcctgtt atttacagtt
     2941 tacaagaaat tgattgattt cctttaccgg aaattaatta gccattcctc tcccatgaaa
     3001 aaagagctga caatattaat tgtcgacgac aataagaatt ttgtggatcg gatgattagt
     3061 ctgctggagg aagtcacaac taataaacgt atcagtacag cttcaaatta tgaagaggcc
     3121 cgccgtttat tgtcaggtga acaacaggat attgtgttgc ttgatatcaa tatgccgggt
     3181 aaaaatggaa tagaactact aaagtttata atgcaaaaaa ataatcagag tgaagtgatt
     3241 atgataacca atcatgctga tacttattac aaagagcagt gtactgaatt gggagcaaaa
     3301 tatttcctgg acaaatcaaa tgattttgca ttagttcccg gaattgtaaa agaattcttt
     3361 ttaaaaaagt ataagaattt taaataaaag tagctgaagc gtatgagagc agagtggaga
     3421 acaataagta atattatcag agtcagtgat tcgccgaaag ataaattcat atccctgata
     3481 gcagaggatg gaactattat gaatgcgaat gccagaatgg tcaggtccct gcacataaaa
     3541 aacccgaaat caagctcagt taattttttt gatctgcttc acccattaaa taaaagtgat
     3601 tttaagaatg gtttcaggca atctatcgaa ggagggtcac ctgtagctat ggaagggtac
     3661 ctgaagaatg gttattacca tcctatgaaa tggcagatct caccggtcat aaaagaaggc
     3721 aaaccggtgg tttatctctg tattggccat aaacttattg atgatgaccg actgcataaa
     3781 tttaaccggc ttggagaaaa taattatcag cttatagtag agagcctcaa tgcaggaatt
     3841 ctattccagg atagcaatgg agatatcatt gctgttaatc aaaaatcggc tgaaattttt
     3901 aatgcaactc tggaacgact ttatcaactg aaagatgttg ctaacctgtg gaataccgcc
     3961 tgggatatca aaaatgaaaa cggagatccg gtcccctttg aaaatacgcc atttatgaag
     4021 gcgttacaat caggcaaaac gcaaacagaa gtgctgctca tcaggctatt ggatggccaa
     4081 tgccggtggc tgcacttcag ttcgcaaccc ctgttcaatg aaaatgctga tatgcctttt
     4141 gctgtcgtat caaacattac agacctgaca tacgaaagaa cgcttatcag ggagttaaaa
     4201 gatggaaagg caattcttaa ttcattcatc aatcaaacac ccaatctggt gtgggtagtt
     4261 gacgaacttt ctactttact ttttgccagt aaggcatttt acaaatactt cggtctgaaa
     4321 gcagcagagt ctgtaaataa aaatattatt gacctggttc ctgaatcagt ggccaaaagc
     4381 ctttacgaga aacatatcca ggttttggag actggcatgc cggctgaatt tatcgagaaa
     4441 gtaaaatggg cggacggtac tgattttatt ttccatataa atatatttat tattgagggc
     4501 atatctaata agcgaatact tgggggacat gctgtcaatc tggctgataa atatgcaaca
     4561 gaacaaaagc tccgggaggc aaatgagcgg ctcttactgt tgacaagagc tacatcagat
     4621 gctatttggg aatgggatat gcaaacaggt catatattcc gcaatgacgc tttaatggac
     4681 atgatcggat atcacacaga tgctccaaag ggcctgtcct ggtggttacg ccgtattcat
     4741 cctgatgacc ggaaccgtgt cgcagataaa gtaaaggata gcacagataa tgggagacag
     4801 tcatgggatg atgaataccg gtttaaatgt gctgatggta cttataagca catgcgggat
     4861 aaaggctaca tagtctatga aagcggatta ccggttaaaa tgattggctc attacaggat
     4921 attaccggtt tgaaagagct ggaatgccag ttgatggaag aaaaactgga acgacaacgg
     4981 gaaatatctg agatgatgat ccgtgcacaa gagaaagaaa gaacccgtat cgggcatgag
     5041 ttacatgaca atgtaaacca gatactttct tcgaccaagc tttttattga aatgttgaaa
     5101 ccatccggaa aagagcaaaa ggattttaaa aataagagta ttgaatatat cagcctggct
     5161 cttgaggaaa tccgaaaatt atccaaagaa ctggttgttc cacaattaaa agataatagc
     5221 ctgataccgg ctgtaaaaca aataattaat gatatacatc tttctactcc tatcttaata
     5281 aaatttgttc atgatcatga agctgattta ctgacagccg caaaaaaagt aactcttttc
     5341 cgtatactgc aggaacagtt gaaaaatata atcaagcata gccgtgcggg caatgtttat
     5401 atttcgcttc agcgtaatgg agctgatatt gcattagtaa ttaaggatga tggtatagga
     5461 tttgacctta gccagacaca caggggtatt ggcctttcaa atatttatga acgggtacgt
     5521 ttttataatg ggactgccga tattaaaact gctcccgggg aaggttgcac acttactatt
     5581 gtgattccaa tgctgcaatg acccgtgtat tgttttttaa atttcaaata ataatattct
     5641 ctgaggggag gttgtatttt atttatgcct ttttgctaag atttgaacaa catcttccaa
     5701 tgaatgattc ttagcctgca gcaatacaag ataatgaaag agaaggtcgg cagcttcacc
     5761 aagaaatagt acatcattag tgtctttgct ttctattact aattcaacag cttcttcccc
     5821 tactttttga gcaattttat tgataccttt tccaaatagg gatgatgtat aggatgaatc
     5881 agaaggccta ttctttcggt ctgcaataat attttctagt tttttcaggg taaagcctgc
     5941 attgtcttca ttaaaacagg tatcagctcc ggtatgacaa accgggcctg cgggattaac
     6001 ttttatcagc aatgtatcat tatcgcaatc tgaaatgatt tctttgataa aaaggaaatt
     6061 gccggaagta tctcctttgg tccagagccg ttgtttacta cggctgtaaa aagtcacttt
     6121 cccttcctgg ttggatcttt ggaatgcttc tgcattcata aatccaagca ttaatacttt
     6181 cccggtgttt gcatcctgta taatggtggg tacaagaccg tcactatatt ttgaaaaatc
     6241 aggtatcatt ttatattcag tttttatctt ctttattttt tagggaattc agcaggctgc
     6301 taaacaattc cttccttcgt tcaaagtata ttttattccg ggttcttctt tttttctcta
     6361 cataccagaa tatgtaataa ctgaaaagca gcgttatgaa aatgataatg atcatattct
     6421 taccggaata tgctggttat ataaatagcg ctttaaatca ggaatagcaa tttccctgaa
     6481 atgaaaaata cttgctgcca gtgcagcatc tgcattggct gatttaaata cttcagtaaa
     6541 atgttccatc tttccggcac ccccggatgc aataaccggg atatttacgg cagttgccac
     6601 tttttttgta atctcaatag caaatccatt ctttgttccg tcattctcca tggaagttaa
     6661 aagaatctct ccggcacctc tttctgtaac ttctttggcc cattcaatcg ttaatttttc
     6721 tgttggtgtt cttccgccat gcgttacgac catccattgg tggttaatta attttgcatc
     6781 aatggcgacc accacacact ggctaccaaa attctttgcc agctcatcaa ttaattgcgg
     6841 attctttgca gctgaagtat tgacagagat cttatctgca ccattttcta aaagaacact
     6901 cacatcctct gaagtattaa tgcctccacc aacagtgaat ggtatattta tatgtgtcgc
     6961 aatctctttc accagtttag ccaatgtttt tcttttttca gttgtagctg ttatatccaa
     7021 aaacactaat tcgtcagcac cttcttcgca ataacggact gccaattcca ccgggttgcc
     7081 ggcatcacgg agttgaataa agttggtgcc tttaaccgtt ctgccatctt ttatgtcaag
     7141 acatggtata attctttttg taagcatttt ttaaaagaat cttttaagat cgcttaaagc
     7201 aatcctgttt tcataaatcg ctttgccaat aatagcaccc ttacaaccag cctggcggag
     7261 gtcttccagt tcttccagtg aagtcacacc tccgctggca atcaggttaa tagaaggatg
     7321 ttgattcaaa atctttttat ataagtctgt tgccggtccc tgcagtaatc catctttgtt
     7381 tacatctgta caaaaaaaat ctttcacccc ttttaatttg tatttttcta tcaattcaaa
     7441 tacagtgatt tttgttgtct ctgtccagcc tctgatgaca attatctcat cttttacatc
     7501 agcaccgata ataaatttgt cagcgccaaa agccatcagc cagccggaaa gagtaaattc
     7561 atcttttact gccatactgc caatagcagc ataagcggcg ccggaaccaa atgcgatttc
     7621 aacattcttg agtgtactga tccccccgct gaagtccaca agtaaatttg ttttaccggc
     7681 aatcttctct agtattttcc aatttttcac ttcaccggct tttgctccgt ccaggtcaac
     7741 caggtgtaac cgctgcaggc cggcgtcctc aaattgctga gccacttcca gcggatcttc
     7801 attgtaagtt gtttgctgac tgtagtcacc ttttgtcagg cgcacacatt taccatcaat
     7861 gatgtctatg gcgggtataa ttgtcatttt attttcaaaa aatttttcaa gatttcttcc
     7921 cctgcctttg aactcttctc cgggtggaat tgtacaccat agaatttatc ttttttcaat
     7981 gcagaactat aaggttgtat ataatctgtt tcagcaatgc taaaagggtt ctgttcagca
     8041 aaatagctat gtacaaaata gcagtaagtg ttttcatcaa cggaagaaaa caattcgctt
     8101 ttcagattgt atatattatt ccatcctacc tgcggaactt taacattggt attaacccca
     8161 ttaaatttcc tgaccattac atttttaaat atccccaggc aatcagtatt attttcttcg
     8221 gaatgttcac agagtaattg catacccagg cagatgccaa ggacaggttg ttgcaatcca
     8281 acaataactt tatccaattc tctttctttc aggtaacaca tggcggtact tgcttcacct
     8341 accccgggga aaattacttt atcagctgtt tgtatcaatt ctttatcgtc cgttactgta
     8401 gccgttgcgc caattcgttc cagggcaaaa agtaccgatt gaatattacc agcattgtat
     8461 ttaactataa ctatattcat tacagaattc cttttgtgct aggcaattgc atattgtcaa
     8521 catccctttt aactgccatt tttattgatt tcgccagtgc tttgaaaatg gattcaatct
     8581 tatgatgttc attatcacct tctactttta tattaagatt acactttgca gcatcagaaa
     8641 aggatttaaa aaaatggtaa aacatttcgg taggtacatc accaattttt tctcttttaa
     8701 aatcggcatg ccaaaccagc cagttcctgc caccaaaatc tatggcaacc tgtgccagcg
     8761 aatcatccat tggtaaggca aatccatacc ggccaatccc ctttttatcg cccaaagcaa
     8821 gggcaaatgc ttcgcccaat gcaattgccg tgtcttcaat gctgtgatgc tcgtcaatat
     8881 gcaggtcgcc ttttacggta attgtaatat ctattccgct atgcctgcac aactgctcaa
     8941 gcatatgatt gaaaaaatta aggcctgtat ctatattggc aacccctgtt ccatccaggt
     9001 ttaacaatat ccggatcttt gtttcgttgg tgtttcgttc atgctgaatg actctttgcc
     9061 caagccggag atattcatat atggatttcc agcttgtagt ttccagggca ataatttctt
     9121 caaagtgctg atctgtccta cttatttctt ctgctccaag tagtgggtca ttattaagcc
     9181 atatggcttt gcacccaaga ttttttgcca gttgtatatc tgtaatcctg tcgccaatga
     9241 caaatgaacc agttatgtca tattccgggt tatttaaata ttgtgtcagc ataccggtcc
     9301 ctggtttacg ggttgggata tcatctgcag caaatgtttt gtcaatatgg atattgctaa
     9361 aaatgatttc ttcgttttcc aggcttttta atacaaaatt ctgaataggc cagaatgtat
     9421 cctcaggata ggaagcagtt cccaatccat cctggtttgt taccattacc agttcaaaat
     9481 ccaggtcttt ggctattttt cgcacccagg taaacatatc cggatagaac tccagttttt
     9541 caaaagtatc aatttggtaa gtaggcgggg cttcttttat aagtgtgcca tcacggtcta
     9601 tgaaaagaat ctttctcatg ctgtatatga ttttaatgct tctatcagtt gtttattttc
     9661 ttccggggtg ccgatggtga tgcgtagaga atcatcacat aggatcactt tagaacgatc
     9721 acggacaatg attcctttgc ctgataaata ttcatatata ttcctggctg ccctcatttt
     9781 tacaaggata aaattggcat cggaaggata aacatattgt gtaaatggca gtttcagcaa
     9841 ttcttcctgt agtaaccctt tttgttcaac ggtagttttt gtccagttat ttacacttga
     9901 aatattacca agagcttcta tggccaattg ctgtgtggcg gtattaatat tgtatgggta
     9961 tttaaccttg ttgaagtaat ttattaccgg ttctccggca aaagccatac ccagtcttaa
    10021 tcctgctaat ccccatgctt tggataatgt ttgtaaaata acaagatttg catattctgt
    10081 aagttccgga ataaatgttt tttgttttgc atagttgata taagcttcat caattatgac
    10141 caggccatca aagttattca acaggacttc gatatcgtct ctgttaatgc tgttgccagt
    10201 tgggttattg ggggaacaaa caaagattag tttggtattt gcatctatgg cttcttcgat
    10261 ggcccccagg tttaactgaa aatcctgtgt taaagggacc ttttttactt tcacagcatt
    10321 catttcagca catacttcat acataccata agtaggaggg aagatgatga cattgtcttt
    10381 accgggttcg cagaatatcc ggaacagtaa gtctatcgct tcatcacttc cgtttccgag
    10441 gaaaatattt tgtgccggaa caccttttat gctgctgatc ttttctttca gtgttaattg
    10501 taaaggatcg gggtatcggt tataattttc agggagaggt gagccaaatg agttttcatt
    10561 ggcatccagg agaatagttg cggtaccgct gaattcatgc cgggctgatg aataaggggt
    10621 catctttttg atattctccc gaacaaggtt atctacatta aattccatag ttattttttt
    10681 aaccggatgg ctactgctct tttatgtgca tccagacctt ctgccccggc cattgccatg
    10741 actgccggag caatattttt taatccttct tttgttaatt tctgataggt gatttttttg
    10801 acaaagctat ctaaagaaac gccactgtac atagcagcat agccattggt aggtaatgta
    10861 tggttggtgc cactggcata atcaccggca ctctccggcg agtaattccc aataaataca
    10921 gaaccggccg tcgttacttt ttgtgcaact tgttctgcat cagcacagga aagtatgagg
    10981 tgttccgggg cgtagcaatt tgaaagttca attgctatag caatattttt tacaataact
    11041 attttactgt tatgcaatgc tttttcagct attgattttc ttggcagttc ttttagttgt
    11101 tcgtttatcg ctgtggctac attctcagca ataccagcag aagttgtcaa tagaattact
    11161 tgtgaatcgg tcccatgctc tgcctgcgat aaaagatcag cagctacaaa ttcagggaca
    11221 gcggtctcat cagcaattac cagtacttca gaaggtccgg ctggcatgtc tatggctaca
    11281 ccttgttgtt gaattatttg tttggccaaa gttacatatt ggttacccgg cccgaatatt
    11341 ttaaaaactt tcggaattgt ttctgtgcca taggccatgg cagcaatggc ttgcacacca
    11401 ccagctttaa atatttttgt tattccgcat agttgcgcag caaacaaaat ggccggatta
    11461 atgctcccgt cttttgcagg tggcgtagaa agaattattt ctttgcaacc agctatctgt
    11521 gcaggaacag caagcattaa aacggttgaa aagagcgggg cagagcctcc gggaatgtat
    11581 aacccgactt tttcaattcc aacagcttct ctccagcaaa gtacgccggg catggtttca
    11641 acctttttca cctcttcttt ctgtgattgg tggaattgtt caatattttt ctttgcctgt
    11701 tgtatggcct gttttaactc ttctggcagt aaagaagctg cagtattgat ttctttttca
    11761 gacacctgta ttctttttaa actggctcca tcaaattctt ttgagaattt tcggattgct
    11821 ttatcccctt tatcttttac tttttgcaat atttttttta cacttttttc cagggaagaa
    11881 tagtccgagg ccggccgctg cattatttct tcccagtttc ctttgtcagg gtatttgtaa
    11941 accttcatca tataatcatt ttttcaatgg ggattattaa tatgccctgg gcaccattgg
    12001 cttttaattg ttcaataaca tcccagaagt cactttcgtt gatcacagag tgaacagaac
    12061 tccatccggg ttctgccaaa ggcagtatgg tagggctttt cataccgggt aataaactgc
    12121 aaatggtatc cagcttgtcg ttgggggcat tcagcaatac gtatttatta ttttttgcct
    12181 ttttcactga gcggatacgg aataacagac tttcaaggat gatccttttt tcggtggaaa
    12241 gagctttgtt accaatcaac acagcttcag agaaaagtat tgtttcaatt tcttttagtt
    12301 cgttggtgaa taaggtactt ccggagctta ccaggtcaca aatagcatct gccaacccaa
    12361 tcctgggtgc aatttcaacg gaaccgctta tctcatgaat agaagcggct atcttttttt
    12421 cattcaaaaa agtagtgagg attacagggt agctggttgc tatcttcttg ccgtttagat
    12481 cacttatgga ttgtaattgg ccattcctgg ggatagcaat ggatagacgg cacttcccga
    12541 atcctaattt ttctatggag tcgacaattt tatttttttc cctgacaaca ttctcaccta
    12601 caaagccaat atcggccact gcatcctgta cgtattcagg gatatcatcg tctcgcagaa
    12661 agaacacttc caccggaaag tttgaagaca gtaccctgag ttggttattt ccgttgctaa
    12721 cttcaatgcc gcattctttc agcagtttca tggaaccttc atataaacgg cccgatttct
    12781 gaacggcgat ctttaatgtt tcactcatat agatatattt aattaaaaag ggcttactgt
    12841 taagtaagcc cttcaactat gtttatacat aatatcctca cggcctaccc tggaagagta
    12901 agtgatgatg atggttatgt gtatattttt tcattacggg gcaaaaatag ggattattta
    12961 aataggtata atatttcact gtaaattttt aaattagacc gatcttttaa aaccacatgg
    13021 ctaaattaac aagaactact aacagcggac attggcatcg cctggggata ttcaatccgt
    13081 ttgccccagg ggcagagaca atagacccgc tgaagccctg gaagatcatt tcccagaaca
    13141 atattttcat tgtttattta tccatactac tgttcgcttt ttatgctttt acccggcatg
    13201 gtctcaactg gatcgcttta tctacttcgc taattgccag tatcccggtt ggatttgcca
    13261 ttgcattttt tgaaacgcag gcgatggagt tcagacgaaa acaccggcgc accggttttt
    13321 ggtattattt attgtatcgc acactccgga ttaccttctg gtttatggtg ctattcttta
    13381 ttattatgaa tatcgttcta tggttaaata ttaataccta cgaaacaata ttcccgcata
    13441 gcctgcatga tttttttgcc accagaaaac tgtttgaatt tctcgggctg tctctaatca
    13501 tttcactggt atctaatttt ttagatgaat tgcaccgaaa gctgggcagg gatgcaattt
    13561 tcaacctggt atttggtaaa tatcaccagg taaagcagga agaccgtatt tttttattca
    13621 tcgacctaaa tcattcaact gatatagcag aggaaatggg agaaatggaa tacagtcact
    13681 ttttgcagga ttattattat gatatcagcg aaccgattgc ccgttacatg ggaagagtat
    13741 accaatatgt aggagatgaa gtggtagttt catggccggt taagaaggga ttaaggtttg
    13801 ccttatgtat tcgttgtttt tttgccattg aaaagcaaat aaacaggtac cgggaacaat
    13861 accttgaaag atacggaagg gtaccggagt ttaaggcctc cttgcatgga ggttatgttg
    13921 ttgtatcaga agtcggcaag tataaatctg aaattgccta tcatggagat gtgttgaata
    13981 ctacagcccg catgctggga aaatgtcatg atctgaatgc cacttttata atatctgact
    14041 ggatcatgaa aaacgtaatt gcaccaaagt atttaaaaat ggaatcgctg ggtctttttc
    14101 aactcaaagg aaagcaacag gaagtggagt tattttctgt ttatatgtcg cagaagaaac
    14161 aggaagagaa aaaaataggg cgaaaaggtt ttttcagacg aaaaccagga gttgattaag
    14221 ctacgatgag ttgctcctgc aagtcatcgg ctgtaatgcc caggtgactc tcttcataga
    14281 tacattcccc gtttttaata agcaatatct ggggtgattc atgcggaacc ctgaatagtt
    14341 cagcaatctt attagacaat tgcctgtaag acagtaaatc gagatagaaa aaatcactat
    14401 tagatagtgg gcccgacttt ttcagcctgc ttaatgcaat actgctgaca ctgcaacggg
    14461 tactatgttt aaatattatc tgaggtctat cgtaagatga cgcaataata tctttcaact
    14521 gattttctga agtaaggtca atccaattca taattgggaa aatatcagcc tttaaacttg
    14581 taattagcct gtggattacc tgggcaacca tatttttgag atgagcagga aacgcctaga
    14641 atgactaacg caatgagtgc cagaacaatt aatgatttat ttttcatata tcgtggatat
    14701 ttgacagatt aacgatttag tctgctgttt attttgtgat gacaaagata agtaacatgc
    14761 aggattttgc ggccccaatt atttatgcta attactgtaa gattgattta gataaagact
    14821 tggttttctt tacttcatga tagtggtcta ctttcaaatt tgattttgtc aggtatgaca
    14881 aactgttaac tttaccgcca gaaatattct ggaaattaga gaaattacta acctgatgtt
    14941 acaactcaaa aaaccccttg catttattga tctggagaca actggcgtaa acccggggac
    15001 tgaccggatt gttgagattg ctattattaa aatccttccc gatggggccc ggagtgtgaa
    15061 aaggaaactg atcaaccctg agatgcctat ccctaaaaat gccagtgaca tacatgggat
    15121 cagcgatgaa atggtgaagg atgcccccac ttttaagcag gtggcgcatg aattgaaaca
    15181 aatgctggat ggttgtgatt ttgccggtta taattccaat cgttttgata ttcccttatt
    15241 aatggaagaa tttttaaggg cacaggtaga ttttgatatg aagaaccgaa agttgctgga
    15301 tgtgcagaat atttttcata agatggagcc caggaccctg agtgcggctt acaagtttta
    15361 ttgcagtaaa agtcttgatg gtgcacatag tgctgaagta gatgcttccg ctactcatga
    15421 aatcctggag gcgcagttag agcgatacac tgaattagga aatacagttg attcgatctt
    15481 aaaagtgatc ggagaagacc aggtagtgga ttttgcccgt cggtttgtaa tggaaaacgg
    15541 aaccgaagtt ttcaattttg gaaaattcaa aggccgtccc gtggcagatg tgttaaaggc
    15601 ggagccccag tattatgatt ggatgatgaa aggtgatttc cctcaacata ctaaacaaaa
    15661 actcaccgaa atttataccc ggacgctgtt gaaaaagggc tgatgcggct ttttgcttct
    15721 ggaaatttta acaggacatt ctttgatgat tatcgtaaat tagaggttca caattatata
    15781 aatccaacag tggagaaact ggttgtcata ccaacatata atgagaagga aaatattaca
    15841 agtatccttc acgccatttt taatctgcat gaagattttc atgtattggt tattgatgat
    15901 ggttccccgg atggaacagc cctgatcgta aaagaattac aagcaaaatt tccaggtcag
    15961 ttatttattg aagaacgaaa aggaaaactg ggattaggta cagcttatat tcatggcttt
    16021 aaatgggcta tagccaggga gtatgctttt atatttgaaa tggatgccga tttttcacat
    16081 aatccgaatg acctgccaag gttatataat gcctgtaaaa atgaaggggc tgacctcgca
    16141 attggttcaa gatatgttac aggcggggga gtggtgaact ggcctcaaaa ccgtattgcc
    16201 ttatcaaaag gaggttcttt atatacaaga atgataacct ggatgccggt aaaggacccg
    16261 acagcaggtt ttatgtgtta caaaaaagaa gtgctggaaa ccattaatct cgataagatc
    16321 gagttcgtgg gatatgcgtt ccagattgag atgaaatttg ccgcctggaa actgggattt
    16381 aaaataaaag aagtgcctat tatattccag gacaggacct atggtgtatc taagatgaac
    16441 aaagggatta ttaaagaagg tgtaattggc gtattaaagt tgcgctggca tagtttattt
    16501 aaaaactacc gcaaaagact taagaatact gatgcagaaa tttccccatt gacaggaatc
    16561 cgcaaaaatg agatgttggc ggagagtgaa tagttattac cgtttcttcc cctcttttat
    16621 ttagtttgca ggccaatgaa aaaggccttt cttcaattac acatcgctgt tttcctggct
    16681 gggtttacgg gtatattggg tcgcctcatt accttaaatg aaggaatgat cgtctggtac
    16741 aggcttcttt ttacggctgt tactatgtgg attctgttcg ggctgatgaa gaagttacag
    16801 aagataccct ttattgatat tttaaaaatt accggtgtcg ggtttattgc agctatgcac
    16861 tgggttactt tttatgggtc aataaaatac tcaaatgtat ctgtagcact ggtttgtttt
    16921 tccgctatta gtttttttac tgccttgttt gaacccctga tcttaaaaaa gaaaatcaac
    16981 tggttagaat tgttactggg gttggttaca ctcagcggta tttatatcat ctttcatttt
    17041 gatacccggt ttaagacagg tattgttatc gggttaattt cagcagtgct ggcatcctta
    17101 ttcccgattt ataacaggga gtttttaaaa agaatcaatg ttgaaactat gctgacctgg
    17161 cagcaaacag ggggcttcct ggttttgtcg ctattactgc cattctacct gcagcagttt
    17221 ccaattgaaa actttattcc tgaccgcgag aatattggtt ggttattggt acttgcatgg
    17281 ttctgctcag tgattgcttt tcaactttct gctaatgcct tgaaaaaatt gtctgcattt
    17341 actgtaaatc ttacttacaa cctggagccg gtatatggta ttctacttgc ctttgctgtt
    17401 tacaatgaaa atcagtacct gagtaaatgg ttctttgttg ggtttgctat tattgcagtt
    17461 gctttactga ttcatgttta tttgcttttc agagtagaac gtaaactgac agaacatgca
    17521 gctgactgag tatcatcaga aaattataga ttattacagg gatactgaga attcgtataa
    17581 ggattcctgg gatttgaata acagcctggc tatacactat ggatattggg acgcaacagt
    17641 gaaaactttt cgccggtctt tgttgcggat gaacgaaata atgatggaag ctgccgcaat
    17701 aaaagcaacg gataaagtac tggatgcggg ttgcggggtg ggtggcagca gtatttttat
    17761 agcttcctta ttggggtgta aagtgacggg catcacatta agcgaaagac aagtgcaaca
    17821 ggctaatgag aatgccagga gaaaaggggt tgaggacctg gttaatttca gtgtgatgaa
    17881 ttttagtgcc actgatttcg ctgatgccag ttttgatgtg gtttggggtt gtgaaagtat
    17941 ttgctatgcg gaggataaag aacaatttat aaaagaagct tttcggttac tcagaccggg
    18001 tggcaggttg gtagtggctg atggacttgt tactgatttt gaaaacaatg agaatcctga
    18061 cattcgtcaa tggcttgatg gctggcaggt aaattacctg gaatctcccg aaaggtttca
    18121 gtcttttatg ctacaggcag gatttggtaa tattaatttt cgcaatatat ccagggaaac
    18181 agctcattct tcccgtcgtc tttatcgcat ttattacctg gccagtttgt atttgctatg
    18241 gaagaaggtt aatttttcaa aacccgttac ggcaatacaa aagaaaaata tcaaagcctg
    18301 caaataccag tttaagggaa tgcaaaaaag actttggcaa tacgggttaa ttaccggaac
    18361 aaaattaaga tgaccattta aaattcaata ataaaaccga tagtcggaat tacactggca
    18421 tcgtcatttg ctaatatgaa cggaatggca ttagagccat cctgcctgat gggctgtcca
    18481 tctgtggtaa ggaaggccgt attatcagca tttcttttaa atgtatattg aggaaaagca
    18541 gggttccttg cattgtacca gttggtaaca tcgaggtaaa gattaagtgt tgttttttta
    18601 aaattccatt tcttgtctat acgcaggtca ctggaatgaa acgcagccag tttttccgag
    18661 ttaagttgat cataattcag aatcccgatg ctataggtca ggtaattcaa ttgtgaagca
    18721 gttaaatcaa acggagtata gggtgctcca ccctggtaac ggaatttcaa ccctaattcc
    18781 cagttcttat tgaatttgta tccccatata aatgaaagca aatgacgatt atcccaggca
    18841 ctggcaattt ccttcttatt caatccgctg aaacggctga taaaaaaggt ataagacaat
    18901 gttccaaaaa atcgtttggt gagtttttgc tgggcaaata attcgaaacc ataacttttt
    18961 ccttttccgt tggtgatcac atcttcattc ccaattacac caaaatcacc acccatatta
    19021 gccagggaaa ttccatcttt tactgaaaca ggaacattcc tgtattgttt ataaaatgct
    19081 tccagtgtaa aacgggtggt agtgttgggt aaatattctg tcccaagcac atagtggata
    19141 ctgcgtgtat aatcaacatt tttattcatc agcactccac tattatcccg gaaactaagt
    19201 atcgtataag tcggaatttt ggaataagaa ccaatcgatg catttagtgt ccatttgtcg
    19261 gcgagaacat aggaagcgct gatgcgtggt gataaggtgg aaattgggtt catgccatcc
    19321 tctgtaaaag tattcagatc actgcgcaag ccagcactga ttcccaaacg atcatcaagg
    19381 taacgtttac ttgcctggac aaatgctccc atacgccaaa agctgatatc agaattgtag
    19441 cgtacagata tttccggttg gatgatattc ccgttctgat cagttaattc tttccggaag
    19501 cgtttaaaaa tattgttatt gctctttaca tactgcgtaa tgccaccgta agcaagtttc
    19561 cagccattga tggtcttatt gacatcgaag cgcagcttat tttctatctc ctgtgaaaag
    19621 atacctatgc ggcgtttggt ttcattttct tcctcattgt catcaaattt ttcaatttta
    19681 ttatcaaaca tattccggct gagggccagg ttccagtagc cattttttat ttgccggcga
    19741 atgtttgctc ctattgtgta attccattga tgtataggtg cagttgcatc cagtacatat
    19801 aatttttcag gagtggcttt gcggggtgct gcgaatgaaa aatcatcgat ggcacctaca
    19861 cctatcagtg tcagtgtagt tttcctgtca attcgatgat tgaccttgta ttgaaagtcc
    19921 cagtaattgg gccgaatcgg gagatctatc gctttgaata acaattgcag gtacgaccgg
    19981 cgggcggaag ccaggaaagt tgtgtttttt gacaaagggc cttcaaaagt gcctgccagt
    20041 tcggtgccac taagcctgat attgccctgc aggctgtttg gattaccatt acgttgcctg
    20101 aactgcaaaa cagaggataa ggcgttatca taccttgcat caaaggccga tgaactcaat
    20161 tttacatctt ccaggaaact tacattaaga attcctgtag ggcctcctgc acttccctgg
    20221 gtgacgaaat ggttgataac cggaatttct atcccgtcca ggtagtaaac attctcattg
    20281 ggggcgccgc cacgtataat aatgtcattt ctgaatccac ctacactacc ggctgtgcca
    20341 cccacaccgg gtagggcctg tatcacccgg gaaatatcaa aatttccgcc gggattgctg
    20401 cggatttcct ctgttgtcag ccgttgtaca cttaacggtg tttccagact ggcggccact
    20461 gctgtgcggc gggagctttt gatcactaca tcttccagcc gggtgatgga aggttccagt
    20521 tgaactgtca ggtttatttc gtttccggaa gttattacga tattaaataa agtcacttca
    20581 ttgtatccga ccgatgtaaa ctgtaaagta taacttttgg gaagtatgcc actgatccgg
    20641 aatttacccg atgaatccgt ggtagtacca aaggaagtcc cgataacctg caccgaaacg
    20701 gatccaaccg gggtcaaaag gttcttgtct gttactgttc cggtaataga cccctgtcgt
    20761 tgcgcctggg cctggtaaaa cagggttatt gctattaaaa gggaaaaaat cttgatcata
    20821 ttataatttc agtataggaa acagttaaac aattgatttg tttgatttaa cttgtcggtc
    20881 atcattttta tttatgagaa aatccaattt tagcctggtc ttgctgttac ttcttgtaac
    20941 aggactttat gcacaaaaga aaccccttga tcattctgta tacgacagct ggcaacgtat
    21001 cggcgaacga atgatcagca acgatgggaa atgggtggta tacacgatcg atccgcagga
    21061 aggagataat gagctggtaa tacagtcttc cgatgcaaag tataagaaaa cgattgcccg
    21121 tggttacaat cctgtaatta cagaggacag caggtatgca attttcagaa taaagccact
    21181 tttcaaggac acaagagaag caaagatcaa aaagaagaag cctgatgata tgccaaaaga
    21241 ttcatttgct atcgtggaat tgggacaaga cagtgtgtgg aaagtagcaa gagtaaaggg
    21301 gtataaaaca ccggagaaag aggccggttg ggtggcatat catctggaaa aagcaattga
    21361 aaagaaagaa acagctgcca aaaagcctga atccaatggt aaaaagatta cggatagcct
    21421 gaatagcgta attgattcgc tgcaccaggt gattcaatcc attcctaagc aaaaaaagaa
    21481 aaaaagggat gatgatgaag cctttactga tcccggattc tttattgctg aaaatcacga
    21541 tgccgaagga gatgaaacac ccggaactcc caccgaagcc ggaacggatc tcgttttaag
    21601 aaacctgaac aatgggcagg aaaaaatatt taataatgtg cttgaatatt atttcagtaa
    21661 aaaggggggc aggttgctga tagaacaggc aaagaatccc aaggattcgt tgagtaaacc
    21721 ctctgtttcc ctgtacgatc tcagcgaagc aaaagctatt gtgctaagca ggggaggcaa
    21781 tgactttaaa aattttgtct tctcagaaga tggttcccag gttgcatatg tggcagaaag
    21841 agatgcccgg ccaaaagagt tgcagaagtt ttaccgggtg tggtatcata agaccggctt
    21901 ggatactgcg atcttattag cggataagaa ttcagtagga atgaaactcg gtatgaccat
    21961 cagcgaattt ggcaatctga gtttcagtaa atcaggtaac cgactattct ttggtactgc
    22021 accgattcag ccaccgaagg acacttcact ggtagagatg gacctggtga aactggatat
    22081 ctggaattat aaagacgatt atttacaaac gcagcaatta ttcaggctgc aaagagacct
    22141 tcaaaccaat tacctggctg tatatcacct gggtaaaaat gaattgaaac agctgggttc
    22201 tgaagaaatt cccatcatct tcccgaccag ggaaggtgat ggtgaccagt ttgtaggagt
    22261 gactgatttt ggcaaacgca tcgaaagcca gtgggctggt aacaccaaaa aagatattta
    22321 tgccattaat gtaaataacg gctcacaaaa attagtgaag agagatatga cgggagttat
    22381 tactcctcag tatatttctc cgaacggtaa gtatatcatg tggtacgata gcaaggtccg
    22441 ccactatttt gtgtgggatg gtaatacgac caggaatatt accgctaaaa ttaaattttc
    22501 attatgggat gaagagcatg atacacccaa cgacccgtca ccttatggtg taatgggttg
    22561 gcatgaggga gattcggcag tgtatgtgta tgacaggtac gatgtgtgga aatgccctct
    22621 tgctgaaaaa tcaaattcta ttaatattac cggagttgga agaactggaa aaaccagaat
    22681 aagatacctg caaactgatc ctgagtccag gtacctgaaa ccatacgatg atatctttta
    22741 caggctattt catgaaaaag ataaatctga ggagatcatt caatcttcgt taaaagagtg
    22801 gttttcattt gaacaggctg aagccggtgc ttatatgttt gggcaaatca agaaagcaaa
    22861 aaatgccagt actcttttat atacgataga gtcatattcc agaacgcctg atttatttgc
    22921 agatcagtta atcccaaatt caattgacga agctatcagg ctttcttaca taaaccagca
    22981 acaaaaggat tataactggg gcacggctga gttgtataaa tggaaagctt tcaatggaaa
    23041 aatgtctgaa ggtgtattat ataaaccgga gaattttaat cccaataaaa aatatccgtt
    23101 gatcatgtat ttctatgaaa cacatgccaa cactttacat agttttattc ctccgacacc
    23161 aaccggcagc cggttgaata tttcattttt tgttagccgt ggttatgtgg tgttctcacc
    23221 ggatattcgt tatacaatcg gacatcctgc aaagagttgt tatgattatg tggtcagtgc
    23281 tgcacaggat cttgctaaac aaagatggat cgatgcaaag aatatcggca tccagggaca
    23341 aagctggggt ggtatacagg tagcacagct catcaccatg accaatatgt tcaaagcggc
    23401 atgggcaggg gcaccagttg ccaatatgac gagtgcctat ggcggtattc gttgggagag
    23461 tggggccaat cgccagttcc agtatgagaa aacacaaagc cggatcggtg ctacgttatg
    23521 ggagaagccc aatctttaca tagagaactc acctttgttt catgttccta aaataaaaac
    23581 gccattggtc atcatgcata atgatgcaga tggtgctgtt ccctggtatc agggcattga
    23641 attatttacc gccatgcgga gattgggaaa gcaagtctgg atgctgaact ataatggcga
    23701 agcccataac ctgcgggaaa gaaagaacca gaaagatatt tctatccggg agcagcaatt
    23761 ctttgactgg ttactaaaag gggagaagcc gccaaaatgg atcacagaag gagtgccggc
    23821 cgtgaaaaaa ggcaaagact ggggattgga gctggttaat tagtttattc gttgaatggt
    23881 taacggtttt tttcctgtta accattcaat tttttttatg ccgctgtact gataaatttc
    23941 actaagcttc tcttggcaat gggcaacaat gtttcatcta ttgggaatgt aagctcagtc
    24001 tctattaaat aaactgctgg gttcggtaag tctttccgtt gacggatcaa ttccgatttc
    24061 atatgttcat aagtactgct gatgctgcga agcttgttat caataaatgc cgttttgata
    24121 ctgcggtatt tttcatcgtg tttttcaaaa atggaaagcc tgtaatgata gacccttgtg
    24181 ctttttacat tgccgctgct tagaaagaaa tagccttcct gtgtgtctaa tggaataagg
    24241 ccaacgggta cgatgtttat tttttcttcg acaaattcat atatctctgt accattgtga
    24301 atagttgttt tgattttccc ggcagagtat tgtattatgt cttccagttc ctgcatcagt
    24361 tcatcatctt cgatcatttg ctgatagagc acctgtaatt tttccatctg tatcccggtt
    24421 aactttttgg ggaaatgctc ctgcaggtat tttttatttt cccggaaagc cacaatgtta
    24481 ttataatgaa aaatgatatc ggccagttgc gggtagagct tattttcggt aaaatactta
    24541 ttaacctcct gcaggtaagc caggagagtg tactttttca gctcaaaatc aatacggcct
    24601 tcggcaaacc aggtttcgga taatgacttc atagatggcg aattttgttc tcctaattta
    24661 caaaaagatg gtatgaatcc aaatgaagtt taactttaga ttgttaaaaa aaataaggat
    24721 gagagtttta ctgttaataa taagtgttac gttattttcc tgcggaaaca caatctatat
    24781 tgttcgtcat gcagaaaaag ctccggtgga agctggtgcc agccagatga tggcaagcga
    24841 tccgccatta tctgatcccg gaaaactgag ggccatagca cttcgtgata aactgaaggg
    24901 ggagaatatc cgctttattt tttcaaccaa tacaaagcga acgatagcga ctgcacaacc
    24961 gctgaatgat cagagaagta ataccagtat cgaaatttac agtagcaaaa aagattcgat
    25021 ggatattttt atcgaaaagc ttaaaactat caggaagggt aatatactgg tggtagggca
    25081 ttctaatacg gtggatgata ttgtaaacag gcttagccaa cgaacagaaa ttgctgctga
    25141 tctgaaagac aacgaatacg ataatttatt tatcctgaaa cggaaagggg atagctatct
    25201 ctatagaaac gaaaagtacg gaaacaaatc tccggattaa tccatgcctc ccggaacttt
    25261 ttcacccgtt ttctttttcc actgcatctc aaagaggaaa gatagctgaa gaaaattttt
    25321 tcctgtttca tcactgattt tgtaatcaaa ttggtgcaga tagccggcct gtaatgtaaa
    25381 gaagggtgag aattcatacc cggctcccag gaataccctg ttccgttcaa agtaaggtgc
    25441 cctgttggta aagaatattt cattggaagc tgtcaggtaa taagttccgg cttctatctt
    25501 tttattgttt agtggtaaag cggaattgat cctgtaccgg aaacggttac gaaaaccatc
    25561 actggtccat ctttgttcag ctctgtagcg atgctccagt tttagtcttt tttcatactg
    25621 ggtcatatta aattgaatcc agctgcgcca ttcatcatta accattggag acttaaaatt
    25681 tccacccggg gaataagtat cgaaaattcc aaaacccgct gaaatggtag aagctttgct
    25741 aattgcaaaa ctggccccgc cttttatttc ataataatga aaatcatcat aaaaacgtag
    25801 tgaacggagc tgcaattccc caaagaggtt ccatttttca gtaaggtcca gttttgtatt
    25861 gatgatgttc cagctccccg tttgtgctgc acccggctga ataaaaaaca aaaaaatgag
    25921 gaatcgaaag tatttcatag tgaatatgaa aaagcttctg tgatgggcag aagctttatt
    25981 aagaattgtt tttaaatgat caaaactctc tttcaggatt gaaattctcc agttccttcg
    26041 ccacagctgt taaaaaggta gcgcctaaac ttccgtccac aatgcgatgg tcgtagctca
    26101 tacttaagta catcatatga cggattgcga tactgtcgcc ggctggtgtt tcaattacca
    26161 cgggcctttt ctttatcgca cccacagcca tgatggccac ctgcggctgg ttgatgatgg
    26221 gtgtacccat aatgcttccg aatgtaccca cattggtaag ggtaaatgtt ccgccggttg
    26281 tatcatccgg t
//
LOCUS       DAORTI010000039        25267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1518_length_25267_cov_7.796208, whole genome shotgun sequence.
ACCESSION   DAORTI010000039 DAORTI010000000
VERSION     DAORTI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 25267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25267
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_25267_cov_7.796208"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..406)
                     /locus_tag="PLO70_07095"
     CDS             complement(95..406)
                     /locus_tag="PLO70_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ74265.1"
                     /translation="MLGFFKKLLGGTTVNYKEMVSNGAIIVDVRTSAEFKAGHIPGSK
                     NFPLDNIRTKVAELKKLNKPVITVCRSGARSGMAKGILKSAGIEVYNGGAWTSLKSKI
                     V"
     gene            complement(412..693)
                     /locus_tag="PLO70_07100"
     CDS             complement(412..693)
                     /locus_tag="PLO70_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ74266.1"
                     /translation="MSSIKELVKNPSTVLVDVRSPWEYEMEHIPGAKNIPLEEIMYKV
                     EEFKSFKSPVVLYCRSGARSGMAVSILKQNGVADVYNGGGLGEMQFLLN"
     gene            complement(713..2113)
                     /locus_tag="PLO70_07105"
     CDS             complement(713..2113)
                     /locus_tag="PLO70_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ74267.1"
                     /translation="MFFQHIYDKSLAQASYFIGCQKAGVAAVIDPKRDVDTYIEIAKQ
                     NNMKITHIFETHIHADFLAGSRELAALTGAQMYLSAEGGPGWEYEFPHIGLKDGTIVK
                     VGNLKLEVLHTPGHTPESISFLLTDTPASDEPVMLFTGDFVFVGDIGRPDLLEKAAGL
                     KGTQDAGAHQMYKSLQRFNALSDFIQVWPGHGAGSACGKALGAVPSTTVGYEKARNWA
                     FRYANNEDGFVKFLLTDQPEPPKYFAMMKKLNKVDRPLLTEVPKLKKLSNDELKEAMA
                     KGIKLIDTRNKTEFEKGFIPESINIQGNNSFATWAGWFLTYEEPFILLADESQLDDLT
                     RKLMRIGLDNIYGYIPSVNEWEKAGGRLNNAKVISLSEFKPLYENNGIQLVDLRGVAE
                     YNSGHIKGAENIFVGSLLNNLDKISKDKKVVIHCQGGDRSSIAYSLLAKNGYANILNY
                     SAGMNEWINEGNPVIF"
     gene            complement(2177..3595)
                     /locus_tag="PLO70_07110"
     CDS             complement(2177..3595)
                     /locus_tag="PLO70_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ74268.1"
                     /translation="MKVEQIYTGCLAQGAYYIESNGEAVVIDPLREVEPYIQKAERNN
                     AKIKYVLETHFHADFVSGHLDLSKKTGAPIIYGPNAKPSFEAHIAKDGEEFKVGKVTL
                     KVLHTPGHTMESTCYLLKDENGKEVGLFSGDTLFIGDVGRPDLAQKVVADLTQDKLAG
                     YLFDSLRNKIMPLADDIIVYPAHGAGSACGKNMSKETTDTLGHQKETNYALRANMTKE
                     EFIKEVTTGLVAPPAYFPLNVMMNIQGYESIDKVLERGQHALSPDAFEAAANETSALI
                     LDTRDPQTFANGFVPNSINIGIDGQFAPWVGAMIPDIKQEILLVTDEGREEEVITRLA
                     RVGYDFTIGYLKGGFNTWKNAGKEVDQIASVDVNELSEIMNKQKVNILDVRKKSEYLS
                     EHIVDAENAPLDFINDSMAQIDKKKTYYVHCAGGYRSMIFNSILRARGFDNLVDVKSG
                     FKALKESGMFKVSDYVCPTTLL"
     gene            complement(3669..4481)
                     /locus_tag="PLO70_07115"
     CDS             complement(3669..4481)
                     /locus_tag="PLO70_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQZ74269.1"
                     /translation="MEIIGYIASLVIGISLGLIGGGGSILTVPVLVYLFGVEPVLATA
                     YSLFIVGLSSLVGAVPKYKQGMVNLKTALIFGIPSIAAVFATRKFIVPLIPKEVFTIG
                     DFVVTKSILMMVLFAILMVFASVSMIRDKKKNEETGNGEQKFNYPLILLEGAVVGLLT
                     GLVGAGGGFLIIPALVLLSKLPMKQAVGTSLLIIAAKSLIGFTGDLSNYTMDWKLLLT
                     VTALAIGGIFIGDILSKKIDGNKLKKGFGWFVLIMGIYIIVKELFFPGGGGH"
     gene            complement(4554..5171)
                     /locus_tag="PLO70_07120"
     CDS             complement(4554..5171)
                     /locus_tag="PLO70_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ74270.1"
                     /translation="MNWKQLFPQFEPGLIEIIEKEAVQRSFNAGDIIMRTGQYIKSTA
                     LVLEGRVKIYRENQDGGEFLMYYLGPGQACAVSMICALQSHTSEIMAVAEEDSEVLMI
                     PVQLMDDMMNKYKTWYQFVIQTYRGRFDELLSVVDNIAFRNMDERLEFYLKRYTEKAG
                     KKTIDISHQQIADDLNSSREVISRLLKKMEQRNLVKLHRNMIELL"
     gene            complement(5181..6461)
                     /gene="murF"
                     /locus_tag="PLO70_07125"
     CDS             complement(5181..6461)
                     /gene="murF"
                     /locus_tag="PLO70_07125"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQZ74271.1"
                     /translation="MSIETIYSIFKQHPSVQTDTRKLQPGDLFFALKGDNFNGNAFAK
                     QAIDAGAVYAVIDEPAFEITGKTILVEDVLTTLHQLANYHREQFSIPFIAITGSNGKT
                     TTKELVHAVLSTKYITYTTEGNLNNHIGIPLTILKIKMDAQIAVIEMGANHQKEIAGY
                     CEYAQPTHGIITNCGKAHLEGFGGVQGVRKGKGELYDYLRSHYGTAFVMWDYNYLQEM
                     SSGITSIIKYGIANADVEGRIVSSEPFLEVEITKGAGTGIIRTQLVGDYNLPNVLLAV
                     AIGKHFTVPDEKIALAIANYTPSNSRSQLVERGTNKIILDAYNANPSSMKLAIENFAR
                     FHTSGKVLMLGGMAELGEESLAEHKDIINVIKKDEWSKVVLVGGDFLRVEHPFISFQN
                     AEEAKEWFRKQHFENMHILIKGSRSMKMEKILEP"
     gene            complement(6555..8108)
                     /locus_tag="PLO70_07130"
     CDS             complement(6555..8108)
                     /locus_tag="PLO70_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQZ74272.1"
                     /translation="MRNLTILLSCVVLLASCKGKGLFGKKKYDKSDVTGWNYNDKNQG
                     GFQVSKAKEQGLGPGLVFVEGGTFTMGQTEEDVMGDWNNIPRRVSVPSFYIDRTEVAN
                     VHYREYLHWLTKVFDPTDPANAKILEGALPDTLVWRSELSYNEPMVEFYFRHPGFNDY
                     PVVGVNWRQAKDFCLWRSDRVNEGTLVQKGFINKANQKPGAQQGDNNFTTKSYLLGLY
                     TAPPGKAMQSKKGQPPMAPTMESGLLSPDYRLPFESEWEYAAYGLINQNPRPSLKEGK
                     RGEELQSNKQVYPWSQNVNGLRDTRRGSWQGVFLANFKRGSGDNAGVAGGLNDRAFYT
                     APVQSFYPNGFGIYNMAGNVSEWVEDAYRPLSSLDFDDQPAPFRGNKYQKLYVADSTT
                     MDPSTRYEKDSLGRVKMVDVTDEESKNRRNYQRGNVIDFLDGDSLSQSSYGYGNTTLI
                     HPEKSKVYKGGSWNDRAYWLTPGSRRHLEEDQALSTLGFRCAMNRMGSPEGNKRKTGQ
                     HFKTKRQKR"
     gene            8285..11710
                     /gene="porU"
                     /locus_tag="PLO70_07135"
     CDS             8285..11710
                     /gene="porU"
                     /locus_tag="PLO70_07135"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HQZ74273.1"
                     /translation="MYLNKGLVCFATFFLLPFLGIGQRIYTPNSILANGNWYKISVEK
                     TGIYKIDIPFLASLGINTSGLASGSIRLYGNGGIMLAEANDVPRLDDLTENAIQVVDG
                     GDGVINGSDYILFYAGGPHEWVKDSANLRFIHRKNLYADKAWYFLTVGGNGKRVQLTP
                     ALASPSITVNSFSETIFHELDTVNFLSSGKEWYGEEFTNAPGKTLTRTFTINIPNIIN
                     NTPLMLQSNCVARSIGSGSRFDIRIDNQPVGQQLINALGGGQYDLFVQQSTLLLSAIA
                     SQNNVNITYSYVPGSFNSQGWLNWFELFTRRQISLNGVSQLSFRDWSSVGNINAAFIV
                     NNATAGTQVWDVTDPLNPLRMQGAVSGTDYQFVNNCSVLKEYIAFNGTDFLIPAAAGK
                     ISNQNLHNTSPSDFLIITHPSLLSEAQRLAQFHLQYNGLRTVTVTTEQVFNEFGSGSP
                     DPAAIRDFVKMYYDKYVSNPANKPRYLLLFGDASFDYKDRVSNNTNLVPAYQSSFSND
                     PLSTYTSDDFFGFLEDDEDINSGLVVNDLDVGIGRVPAKNAVEARNFVDKVAAYFASQ
                     SFGPWRNNLTFIADDEDGNLHLQDAELITTTAAVNGLVFNQQKIYLDAFQQESGAGGS
                     RYPQANQAINNQVYNGTLIWNYNGHGGSRRLAEETILDQEIVNNWNNPNRLPLFITAT
                     CDFAPYDNPTVNSIGENILLRPKTGAIALMTTTRLVFAFSNRVMNNNYIQFALQPDGN
                     GIYKTLGDAVKDAKNYTYQTSGDVVNNRKFTLLGNPAMSLGFPVLKVRATKINGIPVS
                     QADTLSATEKIVIEGEVTDQQGSILTGFAGTVYPTVFDKPQMVTTLANDPGSQVAAFQ
                     TQNNILFKGKASVVNGQFSFTFKVPKDINFQYGNGKLSLYAEDGTKDGSGFFTNFIVG
                     GTSNAVGGDIDGPDITAWLNDEQFVNGGITNQNPVLIIKLTDSSGINTTGTGIGHDIL
                     ATLDNDNNQFFILNDYYVGNLDNYQRGEIRFQLPQLTPGPHSIKIKAWDVLNNSNENI
                     LEFTVASDEELELSHVLNYPNPFSTSTRFWFEHNKPGQRLQVSVQVMTISGRVIKTIS
                     QDITTDGNRSAELVWDGKDEYGDRPGRGVYIYKLRVISPDKKTKEVLGKLVIL"
     gene            11981..13138
                     /gene="porV"
                     /locus_tag="PLO70_07140"
     CDS             11981..13138
                     /gene="porV"
                     /locus_tag="PLO70_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HQZ74274.1"
                     /translation="MRPTALKLTAVILLFCSLSSAVKAQGELNVVTTAVPFLRISPDA
                     RSGGMGDMGVATSPDAYSGLWNIGKVAFNQSKGGIGATYTPWLKDLVNDVYLATVAGY
                     YKFDENQALSGSVRYFSLGNIDFTDIVGNTFANYRPREFGIDIGYSRKLSAKSGVGIA
                     LKYINSNLAGGTTVGSTTYKAGSAVAADLAYYHDGKKPSGSGWAWGAVLSNLGSKISY
                     TDNADAKDFIPANLGLGLNYTSKFNESNSISFGIEANKLLVPTPPGDGATSQDIIDYR
                     NKSVIGSWFSSFGDAPDGFGEELREFQLSLGAEYWYNNQFALRAGYFYENKDKGNRRY
                     FTTGLGLKYNVFGFNFAYLIPTGSGVSRNPLSNTLRFSVLFDLDGGAKAAK"
     gene            13289..13774
                     /gene="ispF"
                     /locus_tag="PLO70_07145"
     CDS             13289..13774
                     /gene="ispF"
                     /locus_tag="PLO70_07145"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQZ74275.1"
                     /translation="MSYRIGSGVDFHQLAEGRELWIGGVKIPHHKGSLGHSDADVLLH
                     AICDALLGALSLGDIGIHFPNTDPAYKGIDSKILLLKTIELVNGHGYSVVNVDSTLCL
                     EAPKIKPYVPQMQKIIADILKITVNDVSVKATTTEKMGFAGREEGLMAYASVLLQKAI
                     V"
     gene            13826..14269
                     /gene="dut"
                     /locus_tag="PLO70_07150"
     CDS             13826..14269
                     /gene="dut"
                     /locus_tag="PLO70_07150"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQZ74276.1"
                     /translation="MPVVEVKIINQSPNPLPAYATAGSSGMDVRAHLDVPVVLKPFER
                     SLLPTGLFIELPENYEVQIRPRSGLAIKQGITCLNSPGTIDADYRGEIMIILINLSGE
                     DQVIHPGDRIAQMVIQKVEKCTWRRVEILEVTERNKGGFGHTGKQ"
     gene            14282..15100
                     /locus_tag="PLO70_07155"
     CDS             14282..15100
                     /locus_tag="PLO70_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQZ74277.1"
                     /translation="MIRISIVLVSLIYLLGSCRATKKIQSAIVKKDSVVVAIPPINNG
                     REDSIAFIKENFNQIQKNRIDFTTFSAKMDVDYDDPDGKKMDVNAQIRMYKDSIIWVS
                     ITAILGIEGLRAYITTDSVKVLDKQNKIYIARSVAYLQDITALPLNLSSLQDLLIGNP
                     VFLDSNIISYSKSSNSVSLHGISDLFKMLITIGEPDNLLQSSKLDDLDEQRNRTSYLT
                     YSDYENKKGVPFSKKRTITVSEKKKLGIKLNFKQYEFNETLSFPFNVPKNYKYD"
     gene            15181..16527
                     /locus_tag="PLO70_07160"
     CDS             15181..16527
                     /locus_tag="PLO70_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQZ74278.1"
                     /translation="MRKKILVLLFFAGFGFLAYGQPADDKSQLEKERQEIQRELNEIQ
                     GMYNKVKGQTKQTLGQLNMLNRKINLQEQYIGTISKELRSIDDDIYLSEIEIYRLRKQ
                     LDTLKAQYARTVVYAYKNRSNYDYLNFIFSASSFNDAVKRIAYLKSYRTYREKQVKTI
                     LETQELIAQRKKQQLVRKDQKNVALQSQTKQVQELAVQKKEKDAVVSQLKSKEKELQG
                     QIATKKKRDRDLQNAITAIVRREIEAAKAKAKAEAEAKRKADELAEKKNPEKAPVTAT
                     NPTTNPTTSPVTTAKRNEPAKRPDSYLDFTAKDVALNSSFQANKARLPWPVDNGVVTL
                     RFGNNKIENTLLTFDNPGITIATPSAGVAVKAVFDGEVSGVYNLGDGMAITIRHGKYF
                     TTYSNLSGVTVSKGAAVKTGQQIGRAGKDDDGNGGQIDFILMIETRNVDPLPWLRR"
     gene            complement(16584..17702)
                     /gene="bshA"
                     /locus_tag="PLO70_07165"
     CDS             complement(16584..17702)
                     /gene="bshA"
                     /locus_tag="PLO70_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQZ74279.1"
                     /translation="MKIGIVCYPTFGGSGVLATELGKALAQKGHMVHFITYQQPVRLS
                     EFMPNIFYHEVQVPTYPLFDYPPYETALSSTMVDVIKNHDLDLLHVHYAIPHASAAFM
                     AKQILKKEGKNIPVITTLHGTDITLVGRDKMYAPVVTFSINESDGITAVSENLRDETY
                     KHFQIEKDIEVIVNFVDVSRFNRKPIDAFRKVIAPNGERILLHASNFRKIKRVQDVVK
                     IFAEVSKQIPSKLLFVGDGPERSTAEDLSRELGVCDEIRFVGKQEQMEDILAIGDLFL
                     LTSEYESFGLAALEAMAAGVPVISTNAGGLGEINIPGETGYMSNVGDVENMSKQAISI
                     LQDDDTLKGFKERAAEHAKKFDIHNIVPLYEKLYEKFL"
     gene            17821..19215
                     /locus_tag="PLO70_07170"
     CDS             17821..19215
                     /locus_tag="PLO70_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQZ74280.1"
                     /translation="MRKSSLFIVFTLFTRAILGQQSDSAFIKQISNEILTNGKAYENL
                     RYLTKKIGARLAGSPGMVKSEQWGLKVMQESGADKAWLQECMVPHWVRGGKDVASAFY
                     LQASKQGINVSRIQKELDVVALGNSIGTGKNAVSAEVIVVNSFNELERRSAEIKGKIV
                     FYNYKFNDAYVNTFLSYRDAGQYRGQGPSRAARYGAKAVIIRSMSHATDNNPHTGATR
                     YDSNYAKIPAVAIGLRDADWLSEQVKKGGVSATIKTNGHFLPDTIGHNVIGELKGTEF
                     PDQIITIGGHLDSWDNCEGAHDDGAGCVQTIEILRAFNAIGYKPKRTIRFVLFANEEN
                     GLRGGNKYAEEAKANNEKHILAMESDAGGFTPRALGFSGTEEQFQKFLSWRELIAPYG
                     CSEFVSGGGGADIGPLSRALKTPTASLNPDTQRYFDIHHARSDVFEAVNKRELELGAV
                     NMAALIYLVDKYGL"
     gene            19217..19675
                     /locus_tag="PLO70_07175"
     CDS             19217..19675
                     /locus_tag="PLO70_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74281.1"
                     /translation="MENYYEVSNGEYLITTNPSLLSVDIIHHYLSEESYWAKHIPRVV
                     VEKSITNSLCFGLYRQSEQIGFARLVTDKATFAYLADVFILEKYRGKGLSKWLIEVIQ
                     FHPELQGLRRWMLGTRDAHGLYAQFGWSEISEETRKIFMQRHFPDVYAVS"
     gene            complement(19668..20999)
                     /gene="argH"
                     /locus_tag="PLO70_07180"
     CDS             complement(19668..20999)
                     /gene="argH"
                     /locus_tag="PLO70_07180"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQZ74282.1"
                     /translation="MKLWQKDKASLKEVEIFTVGKDREMDMYLAAFDVLGSLAHIEML
                     ESVGLLTKDELSQLQKELKNVFRQIKKGEFILQDDVEDIHSQVELLLTQKLGDIGKKI
                     HSARSRNDQVLVDIKLFLRNELEELVKTIQSFFELLQSQSEKYKDHLLPGYTHLQLAM
                     PSSFGLWFGAYAESLVDDMLTIKAAYDVVNKNPLGSAAGYGSSFPINRTLTTKLLGFA
                     DLNYNVVYAQMGRGKAERIVAQSLANVADTLAKLSMDACLYLNQNFGFISFPVELTTG
                     SSIMPHKKNPDVFELIRSYCNQIKALPNEIMLMTTNLPSGYHRDLQLLKEHLFPAFKT
                     LKNCIEMAGLMLSNIQVKENILADDKYKFLFSVEEVNKLVNSGTPFRDAYKKVGSDIE
                     ADKFTYTTSVNHTHEGSIGNLGTAEIKQQLEKIIASFPFENVKAAITKLVS"
     gene            complement(20996..22066)
                     /locus_tag="PLO70_07185"
     CDS             complement(20996..22066)
                     /locus_tag="PLO70_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HQZ74283.1"
                     /translation="MSKEISILQDQAVELLKELIATPSFSKEEDQTAGILCRFLGNKD
                     IVHTRVGNNVFALNKHFDEKKSTILLNSHHDTVKPNPQYSKDPFYPSIVEGKLFGLGS
                     NDAGGCLVSLIAVFLHFYNKQDLKYNLILAATAEEEISGSGGIEYTLPYLPKIDFAIV
                     GEPTQMQMAVAERGLMVLDCIAHGKAGHAARNEGENAIYKAMKDIEWFSTYQFNRVSE
                     LLGASKMSVTVIETENKAHNVVPALCKFVVDTRINELYSFEEVIDIIKANVQCEVMPR
                     STRLRSTSIALDHPLVKAGLNLGSAYYGSPTTSDKALMPFPALKMGPGDSARSHTANE
                     YIYIDEIKKGIELYIQLLNQIV"
     gene            complement(22059..22844)
                     /gene="argB"
                     /locus_tag="PLO70_07190"
     CDS             complement(22059..22844)
                     /gene="argB"
                     /locus_tag="PLO70_07190"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQZ74284.1"
                     /translation="MDKLYVIKIGGNIIDDEVKLASFLADFASIEEKKILVHGGGKLA
                     TRLAEKLGIEQQLVDGRRITDAETLKIVTMVYAGFINKNIVAVLQANNCNAIGLCGAD
                     GDTILAHKRQHPVLDYGFVGDVDAINTNLINNLLEKNIAVVFAPITHDQQGQLLNTNA
                     DTIAQEIARSMSDLYNVQLIYSFEKSGVLLDANDDTTVIPEIKPSSYLQLKSKNKIFA
                     GMIPKMDNAFAALKSGVGKVIIGKAENLKGLINGSSGTTIINE"
     gene            complement(22837..23793)
                     /locus_tag="PLO70_07195"
     CDS             complement(22837..23793)
                     /locus_tag="PLO70_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HQZ74285.1"
                     /translation="MRNFVSVQDVSNIDALVQQALAYKANPFADKTLGINKRIGCLFL
                     NPSMRTRLSTQIAAQNLGMEAIIFNVGNEGWMLEFEEEAIMSGTSVEHVKDAAPIFGK
                     YFDILAIRTFPSLKNRDDDYSELYIKQFIKYAGIPVVSLESATLHPLQSLTDIITITE
                     SFNETRKPKIVLSWAPHVKPLPQCVANSFSQWVNSWGKADFVITHPEDYELSEEFTKG
                     AIITYNQNDALKNADFVYVKNWSTFNDYGKIYESDPKWMLNNKKLELTNDAKIMHCLP
                     VRRNVELSDEILDGPNSIVTQEAANRIWAAQSVLAEILKTNG"
     gene            complement(23795..24955)
                     /locus_tag="PLO70_07200"
     CDS             complement(23795..24955)
                     /locus_tag="PLO70_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ74286.1"
                     /translation="MNLFDVYPINEITIVKAKGSYVWDDNGEQYLDLYGGHAVISIGH
                     THPHWVQRIEEQLEKIAFYSNSIRIPLQQHLAEKLGKVSGKPDYQLFLCNSGAEANEN
                     ALKLASFHNGRKKIVAFSRAFHGRTSLAVSVTDNKNYIAPVNETGNVSFLPFNDEEAL
                     EIFFNENGKEISSVIIEGIQGVGGINIADDSFLRKIRSLCDEYGALYIADSVQCGYGR
                     SGKFFSHDFAGVNADIYTMAKGMGNGFPVAGLIIAPHIKPKHFQLGTTFGGNHLACAA
                     ALAVLETIEEEKLMGNAVMTGKFLKDQLEVIPQLKNVRGRGLMIGFDVPEELKDLKKN
                     LLWNQKIFTGEAKPNVIRLLPSLALKKRDAEQFIDSIKEEIQNLVPASNPVS"
     gene            complement(24965..>25267)
                     /locus_tag="PLO70_07205"
     CDS             complement(24965..>25267)
                     /locus_tag="PLO70_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQZ74287.1"
                     /translation="GIFISSTTNCDLSIEELNLLYSTFYAAHPFTYVSKEPIFLKQVV
                     NTNKAVIQLEKAGSKLVVHSAIDNLLKGASGQAVQNMNLIFGLDETTGLKLKANYF"
BASE COUNT         7379 a         5090 c         5005 g         7793 t
ORIGIN      
        1 tcctgaatac ctgctatttt tttaagtgct tctttgatgc ttttcataca accaccgcat
       61 ttaatatttt caacgaaaag cgtttctgtg gtgctcatac tattttgctt ttaaggctgg
      121 tccatgcacc gccattataa acttctattc ccgctgattt aagaatacct tttgccatgc
      181 cactcctggc tccactccgg caaacagtga taacgggctt gttgagtttt tttaattcag
      241 caacttttgt acggatattg tccagcggga aatttttaga accagggata tgtccggctt
      301 tgaattctgc tgaagtgcga acatctacaa taatagcacc gttgctaacc atttctttat
      361 agttgacggt agtgccgccg agcaatttct taaagaatcc taacatagtt atcagtttaa
      421 caggaattgc atttcgccaa ggccaccgcc attgtacaca tctgctactc cattttgttt
      481 taaaatgctt actgccattc cacttctggc gccgctgcga cagtaaagaa ctaccgggct
      541 tttgaatgat ttgaactctt ccaccttata cataatctct tctaagggaa tattctttgc
      601 accgggtata tgttccattt catattccca ggggctgcgt acgtccacca aaacagttga
      661 tggattttta accagttctt ttattgaact cataggtata ataatttata ttttaaaaaa
      721 taacagggtt tccttcattt atccattcgt tcatacctgc tgaatagttt agtatattgg
      781 cataaccgtt ttttgcaagt aaggagtaag cgatagatga cctgtcgcca ccctggcaat
      841 gaatcactac ttttttgtct ttactgatct tgtcaaggtt gttcaataaa ctacccacaa
      901 atatattttc tgcacccttt atatggccac tattatattc agcaacacct cttaaatcta
      961 ccagctgtat gccgttgttt tcatataatg gtttgaattc tgaaagcgag ataacctttg
     1021 cattatttaa gcgaccacca gctttttccc actcattaac cgaggggatg tagccataga
     1081 tattatccag gccaattctc atcagttttc ttgtcagatc atccaattgt gattcgtcag
     1141 ccaatagaat gaatggttct tcgtatgtta aaaaccaacc tgcccaagtg gcaaaagaat
     1201 tattaccctg gatatttata ctttctggaa taaagccttt ttcaaattcc gttttgtttc
     1261 ttgtatcaat cagctttatg ccttttgcca ttgcttcttt taattcatca ttgctcaatt
     1321 ttttcagctt aggtacttct gtcagcagcg gcctgtccac tttgttcagc tttttcatca
     1381 ttgcaaaata cttgggcggc tctggttgat ctgtcaacag aaattttaca aaaccatcct
     1441 cattgtttgc ataccggaaa gcccagttcc ttgctttttc ataaccgacg gtagtactgg
     1501 gtacagctcc gagtgcttta ccacaggcag atccggctcc gtggccaggc caaacctgta
     1561 taaaatctga caatgcattg aagcgttgta atgatttata catttggtga gcaccagcat
     1621 cctgggtgcc ttttaaacca gctgcttttt ccagcaggtc aggtcttcca atatcaccaa
     1681 caaatacaaa atcaccagtg aatagcatta caggttcatc gctggcagga gtatcagtta
     1741 acaagaaact tatactttcc ggcgtatggc cgggagtatg taaaacctct agttttaagt
     1801 tacccacttt tactatagtg ccatctttta acccaatatg cgggaactcg tattcccaac
     1861 ctggtccacc ctcagcagat aaatacatct gggctccggt taaagcagcc aattctctgg
     1921 aacctgccaa aaaatcagca tgaatgtggg tttcgaatat gtgagtgatc ttcatattgt
     1981 tttgcttcgc aatttcaata taagtgtcca catctctttt ggggtcaatc actgcagcta
     2041 caccagcttt ctggcaacca ataaaataac tggcttgtgc aaggctttta tcataaatat
     2101 gctgaaagaa catttgaatt atagtttata gttgaaaaaa taattaaaga gttttaagct
     2161 aaataccaat caatttttat aataatgttg taggacatac ataatcactt actttaaaca
     2221 tgccgctttc ttttagagct ttaaaaccac ttttgacatc gacaaggtta tcaaaacctc
     2281 ttgcacggag tatcgaatta aagatcatgg aacggtaacc tcctgcacaa tgcacataat
     2341 aagttttctt tttgtctatc tgggccatgc tgtcattgat aaaatccagt ggtgcatttt
     2401 cagcatcaac gatatgttca ctcaggtatt cacttttttt acgtacatcc aggatattca
     2461 ctttctgttt gttcataatt tcagacaact catttacatc aactgaagca atttgatcta
     2521 cttctttccc ggcatttttc catgtgttga aaccaccttt caggtaaccg attgtgaaat
     2581 catagccaac ccgggccagc ctggtgatca cctcttcttc acggccttcg tctgttacta
     2641 ataagatctc ctgtttgatg tccggaatca tggcgcccac ccagggggca aactggccgt
     2701 caataccaat attgatagag ttgggaacaa aaccattagc aaatgtttgg gggtctcttg
     2761 tatccaggat taaagcgctg gtttcattag ctgctgcttc aaaagcatca gggcttagtg
     2821 catgctgacc tctttccaaa actttatcaa tactttcata accttgtata ttcatcatca
     2881 cattcaacgg aaagtatgca ggaggggcaa ccaggccagt agtaacttct ttgataaact
     2941 cctctttggt catattggca cggagtgcat aatttgtttc tttctgatgt cctaatgtgt
     3001 ctgtagtttc tttgctcata ttctttccac aggcactacc ggcaccatgt gcaggataaa
     3061 ctataatatc atctgcaagt ggcattatct tattacgaag ggaatcgaac agataaccgg
     3121 ctaatttatc ctgggtcagg tcagcaacta ccttttgggc caggtcggga cgacccacgt
     3181 caccaatgaa caaggtgtca cctgaaaaca aaccgacttc ttttccattt tcgtctttca
     3241 acaggtaaca ggtgctttcc atggtatggc caggggtatg tagcaccttt aaagttactt
     3301 tacccacttt gaactcttca ccatctttag caatatgtgc ttcaaatgaa ggttttgcat
     3361 tcggtccgta tataatagga gctcctgttt tcttactaag gtctaagtga ccacttacaa
     3421 agtccgcatg gaagtgtgtt tccagaacgt atttgatttt ggcgttatta cgctctgcct
     3481 tttgtatata aggctctact tcacgaagcg gatcaattac aacagcttct ccattacttt
     3541 caatgtagta agctccctga gctaagcaac cggtatagat ttgttctact ttcatttgta
     3601 tttttttatt ttaattaaag atcaaatttc gaataagata aagtgttgta cagtgacaac
     3661 aatcacaatc aatggccacc tccaccgggg aagaagagtt ctttaacaat aatataaata
     3721 cccattatta atacgaacca gccaaatcct tttttaagtt tgtttccgtc tattttcttg
     3781 ctaagtatat caccgataaa aattccgcct attgccagtg ctgttacagt cagtaatagt
     3841 ttccagtcca ttgtgtaatt ggacaagtcg cctgtaaatc caatcaatga tttggcagca
     3901 ataataagta aggaagtacc tacagcctgt ttcatgggta atttgctcaa caataccagg
     3961 gcaggaataa tcaggaatcc gccaccggca ccaacaagac cggtcaacaa gcctaccaca
     4021 gcgccttcca gtaatatcag cgggtagtta aatttttgtt caccatttcc tgtttcttca
     4081 tttttctttt tatcccttat catagaaaca gaggcaaaga ccataaggat ggcaaataat
     4141 accatcatca ggatactttt cgttaccaca aaatctccga ttgtgaaaac ttctttcggt
     4201 atcagtggta ctataaattt cctggtagca aataccgcag caatagaagg aataccgaaa
     4261 ataagggctg ttttaaggtt taccattccc tgtttgtatt taggaacagc accaacaagg
     4321 ctggataatc caactataaa taaggaatat gcagtggcta aaacaggctc tacaccaaaa
     4381 agataaacta aaacgggaac ggtgaggata gagccgcctc caccaatcaa accgagagaa
     4441 ataccaatga caagcgaagc aatatagccg atgatttcca tgtaaataat ttttgcaaga
     4501 ttagaccaaa gacctttctc acaatgtgac agaaatcaca gcagtaacag ctactaaaga
     4561 agctcaatca tattacgatg caacttaaca aggtttcgtt gttccatctt tttcaacagg
     4621 cgggatatca cttccctgga actattcaga tcatctgcaa tctgctggtg agagatatca
     4681 atggttttct taccggcttt ttctgtgtat ctcttgaggt agaattccag ccgctcatcc
     4741 atattccgga aggcaatatt atcaacaaca gataacaatt catcaaatcg gccacgatag
     4801 gtttgaatga cgaactgata ccatgtcttg tatttattca tcatgtcatc catcagttgt
     4861 accggtatca ttaaaacctc cgaatcttct tcagccactg ccatgatctc actggtatga
     4921 gattgcagtg cacagatcat tgaaacggca catgcctggc caggtccgag gtaatacata
     4981 aggaattctc cgccgtcctg attttccctg tatattttaa cacggccttc cagtaccagg
     5041 gctgtagact tgatatattg tcctgtccgc atgatgatat ctccggcatt gaagctcctt
     5101 tgtactgctt ccttttctat aatttcaatt agtccgggtt caaattgagg gaagagttgt
     5161 ttccagttca tttggtaaag ttaaggttct aatattttct ccattttcat acttctgctt
     5221 cctttgataa ggatatgcat attttcaaag tgctgttttc gaaaccattc ttttgcttct
     5281 tcagcattct gaaatgagat aaaagggtgt tccactctta aaaaatcacc gccgaccaga
     5341 acaaccttac tccattcatc ttttttaata acattaatta tgtctttgtg ttcagcaaga
     5401 ctttcttcac ccagttctgc cattcctccc agcatcagta cttttccact tgtatgaaac
     5461 ctggcaaaat tttcaatcgc cagtttcata ctgctgggat tggcattata tgcgtccagt
     5521 atgattttat ttgttccccg ttctaccagt tgagaccggc tattggaagg agtgtagttt
     5581 gcgatcgcca atgctatttt ttcatctgga acagtgaagt gttttccaat agcaacagcc
     5641 agcagcacat ttggtaaatt atagtctccc actaattgag ttctgattat tccagttcct
     5701 gcacctttcg ttatttccac ttccagaaaa ggttcactgc ttactattcg cccttctaca
     5761 tcggcatttg caattccata tttaataata gaagttatac cgctgctcat ttcctgcagg
     5821 taattgtaat cccacattac gaatgcagta ccgtaatgag aacgtaaata atcatatagt
     5881 tctcctttgc cttttcttac tccttgtact cctccaaaac cttcaaggtg ggctttgcca
     5941 caattggtaa ttatgccgtg ggtcggttgc gcatattcgc aatagccggc aatttctttc
     6001 tgatgattgg cacccatttc tataactgct atttgcgcat ccatttttat cttcaaaatg
     6061 gttaagggaa tgcctatatg gttattcagg tttccctcag tagtataagt tatatacttg
     6121 gtggagagaa cagcatgtac cagttctttg gtggttgttt tcccattgct tcctgtaatg
     6181 gcaataaatg gtatactgaa ttgttcccgg tgataattgg ccagttgatg gagagtggtt
     6241 aatacatctt ctaccaggat agtctttccg gttatctcaa atgccggttc gtcaattaca
     6301 gcataaactg ctccggcatc aattgcctgt tttgcaaaag cattgccatt gaaattatcc
     6361 ccttttagcg caaaaaaaag atctccgggt tgtagtttac gggtgtctgt ttgtaccgaa
     6421 ggatgttgtt taaaaatact ataaatggtt tcaatagaca tccagcaaaa gtaatccttc
     6481 aataaactcc agacgataac ttaataaaaa aaccctccgg attccggagg gtttaaagaa
     6541 tgtattagaa tcgattatct tttttgtctt tttgttttaa agtgctggcc tgttttacgc
     6601 ttattgcctt cagggctacc catacgattc atagcgcaac ggaaacccag tgtgcttaaa
     6661 gcctggtctt cttcgaggtg acgacgactt ccgggagtta accaataagc cctgtcattc
     6721 cagctgccac ctttgtacac tttggatttt tcagggtgaa ttaaggtagt gttaccgtat
     6781 ccgtatgaag attgagataa agagtcacca tcgaggaagt caatcacatt acctctttgg
     6841 tagttacggc ggtttttact ctcttcgtcg gtcacatcaa ccattttcac acggcctaaa
     6901 ctgtcttttt catatctcgt agatggatcc attgtagttg aatcagctac atataatttt
     6961 tgatatttat taccacggaa aggggccggc tggtcatcaa aatcaagaga agaaagagga
     7021 cggtaagcat cttctaccca ctcactcaca ttaccagcca tgttatatat accaaaacca
     7081 ttgggataaa aagattgtac cggggcggta taaaaagcac ggtcgttcaa accgccagcc
     7141 acaccggcgt tatcaccgga acctctttta aagttggcca ggaaaacacc ctgccagctt
     7201 ccgcgacggg tatcacgaag accatttaca ttttgtgacc atggataaac ttgcttattt
     7261 gattgtaact cttcgcctct cttgccttct tttaaacttg ggcgggggtt ttgatttatc
     7321 aatccataag ctgcatattc ccactcagat tcaaacggaa gccggtagtc cggagacaga
     7381 agacctgatt ccattgttgg agccattgga ggttgtccct ttttagactg catggcttta
     7441 ccgggaggag ctgtgtataa acctaacaaa taagatttag tggtgaaatt attgtcaccc
     7501 tgctgagcac cgggtttttg atttgcttta ttgatgaacc ctttttgaac aagcgttccc
     7561 tcatttacac ggtcactcct ccaaagacaa aaatcttttg cctgtctcca gtttacacca
     7621 acaacaggat aatcattgaa ccccggatgg cggaaataaa attcaaccat cggttcgtta
     7681 tagctcaact cacttctcca aaccagtgtg tcaggtaaag caccttccaa tattttggca
     7741 ttggccggat cagttgggtc aaaaactttt gttaaccagt gcaggtattc acggtagtga
     7801 acattggcga cctcagtacg atctatataa aatgaaggaa ccgacacccg gcgtggaata
     7861 ttgttccagt cgcccattac gtcttcctca gtttgtccca ttgtgaaagt accaccttca
     7921 acaaaaacca aacccgggcc gagaccctgt tctttggctt tagatacctg gaaaccaccc
     7981 tggtttttgt cattgtagtt ccagccggta acgtcggact tgtcgtattt ctttttgcca
     8041 aaaaggcctt ttcctttaca ggatgccagc agcaccacac aggatagtag gattgttaag
     8101 tttctcattg ttttttgcat aaacctatga attggaataa atttaacgcc ggatgaggta
     8161 aaatattgcg gcctgaagaa cttttaccct ccgaactgcg aatatatata ctttggggaa
     8221 taacggttac gcagaaatac ctttttttaa acgggaaaat cccattaaac caatgtttta
     8281 gcagatgtat ttgaacaagg ggcttgtctg ctttgctacc ttttttttac tcccattttt
     8341 ggggatcgga caacgtatct acactcctaa ttcaattctt gcaaatggta attggtataa
     8401 aataagtgtt gagaaaacag gcatttataa aattgatatt ccttttttgg ccagcctggg
     8461 aattaataca tctggtcttg cctcgggttc aatccggtta tatggaaatg gtggaattat
     8521 gctggctgaa gctaatgatg tccccaggtt ggatgacctt actgaaaatg ccatacaggt
     8581 agtggatggc ggcgatgggg tcatcaatgg ttcagattat atcctctttt atgccggagg
     8641 tccgcatgaa tgggtcaaag actcagctaa tcttcggttc atacatcgga aaaatttata
     8701 cgcagataaa gcctggtatt ttttaactgt tggcgggaat ggtaaaagag tacaattaac
     8761 ccctgcttta gcttctcctt cgataacggt caatagtttt tcagaaacaa tttttcatga
     8821 attggataca gtcaattttt taagtagcgg taaagaatgg tatggtgaag aatttaccaa
     8881 tgctccggga aaaacgttga ccaggacttt tacaattaat attccgaaca ttattaataa
     8941 cactcccttg atgctgcaaa gcaattgcgt agccaggtcg ataggctcag gcagccggtt
     9001 cgatatcaga attgataatc agccggttgg tcagcaactg atcaatgcgt tgggcggtgg
     9061 ccagtatgat ttatttgtgc aacaatcaac tttattgctt tctgcaattg cttctcaaaa
     9121 taatgtgaat attacttatt cttatgttcc gggtagtttt aattcgcaag gttggctcaa
     9181 ttggtttgaa ttgtttacca ggcgtcaaat ttccttaaat ggggttagcc aattatcgtt
     9241 cagggattgg tcaagtgtgg gcaatatcaa tgcagcgttc attgtcaaca atgctacagc
     9301 cggaacgcag gtttgggatg taacggatcc gttgaaccca ttaaggatgc agggagcggt
     9361 atccggtact gactatcagt ttgtaaataa ttgttcagtt ctgaaagagt atattgcctt
     9421 taatgggact gattttttaa ttccagcagc tgcggggaag atatccaatc agaatctaca
     9481 taatacttcg ccgtcagatt ttttgataat cacccaccct tcattattgt cagaagctca
     9541 gcgattggca caatttcacc tgcaatacaa tggtttaaga acggttaccg ttaccactga
     9601 acaggtgttt aatgaatttg gaagcgggag ccctgaccct gcagcaatcc gtgattttgt
     9661 aaagatgtat tacgataaat atgtttcaaa tccggccaac aagccacgtt acctgctgct
     9721 ttttggcgat gcttcatttg attataaaga cagggtgagt aataacacaa atttagttcc
     9781 agcctatcaa agcagttttt caaacgaccc gttatctact tatacttcag atgatttttt
     9841 tggttttttg gaagatgatg aagatatcaa ttctggtttg gtcgtgaatg atcttgatgt
     9901 tggcattggc cgtgtgccgg ctaagaatgc agttgaagcc aggaattttg ttgataaagt
     9961 tgcagcgtat tttgcttcac aaagcttcgg cccctggcga aataacctga cttttattgc
    10021 tgatgacgaa gatggaaatc ttcatttaca ggatgcagaa ttaataacaa caactgctgc
    10081 tgtaaatggt ctggtattca atcagcaaaa aatttacctg gatgcgtttc agcaggagag
    10141 cggtgccggg gggagcaggt acccacaggc caaccaggca atcaataacc aggtgtataa
    10201 tggtacgctt atatggaatt acaacggaca tggcggatca aggcgattag cagaagaaac
    10261 catactcgac caggagatag tgaataattg gaacaacccg aaccggttac cattgttcat
    10321 tactgctacc tgtgattttg caccctatga taatccaact gtcaattcta tcggtgaaaa
    10381 tattttatta agacctaaaa ccggggcgat cgccctgatg acaactacaa gactggtatt
    10441 tgcattcagt aatcgtgtaa tgaataataa ttatatacag tttgcgctgc aaccggatgg
    10501 taatggcatt tataaaacac ttggcgatgc ggtaaaggac gctaagaatt atacttacca
    10561 aacctctggt gatgttgtca acaacagaaa atttaccctg ttgggcaacc cggccatgtc
    10621 tttgggattt cctgttttga aggttagggc caccaaaatc aatggaatac cagtttcaca
    10681 ggcagataca ttgagtgcaa cagaaaaaat tgtaatagag ggagaagtga ctgaccaaca
    10741 gggcagtatt ttaactggtt ttgcaggtac tgtttaccca actgtttttg ataaacctca
    10801 aatggtaacc acccttgcta atgacccggg tagtcaggta gctgcttttc aaacacaaaa
    10861 caatatttta ttcaaaggca aagcttctgt cgtcaatggt cagttctctt ttacattcaa
    10921 agtgccgaag gatataaatt ttcaatatgg aaatgggaaa ttaagtttat atgctgagga
    10981 tggtacaaaa gacgggagtg gtttttttac caattttatt gttggcggaa caagtaatgc
    11041 tgttggcgga gatattgatg gcccggatat aacggcctgg ttgaatgatg aacaattcgt
    11101 aaatgggggt ataaccaatc aaaacccggt tttaatcata aagctgacgg attcgtctgg
    11161 cattaatacg acgggaactg gcatagggca tgatattcta gccactcttg acaacgacaa
    11221 taatcagttc tttatcctga atgactatta tgtgggaaat ctcgacaact atcagcgtgg
    11281 tgaaataagg ttccagcttc cacagcttac cccggggcct cattcaataa agatcaaagc
    11341 atgggatgta ttaaataact ccaatgaaaa tatacttgaa tttacggttg ctagcgatga
    11401 ggaactggaa ctaagccatg tgttgaatta tccaaaccca tttagtacca gcacccgttt
    11461 ctggtttgaa cataacaagc caggtcagcg gttacaggta agtgtgcagg taatgactat
    11521 atcgggcagg gtcattaaaa ccatcagcca ggacattaca accgatggta accgttctgc
    11581 agaattggtt tgggacggga aggacgagta cggtgacagg cccggccgtg gggtatatat
    11641 ctataaatta agggtaatat cccccgacaa aaagacaaaa gaagtgcttg gtaagctggt
    11701 aatattataa ttacctgatt ttttggttca gatcagggtc cttatttatt gcaagcagct
    11761 aaaaatccgg tttttgccgg ttaaaaataa attttaacaa taaaatcgaa aagaagacgt
    11821 tttttcagaa atttgcttgt tggcaatagc catcaaatag cttaaaaatc gttgagaacc
    11881 atctaaaatc gacaattttt tcgtttcaga taaaattctc tattttagat ttgtatttat
    11941 tcaaaagctc tatatttaca ccattattaa aaattttcta atgagaccaa ctgctttaaa
    12001 actaactgcc gtaatcctgc ttttttgtag cttatcatcc gctgtaaaag ctcagggtga
    12061 acttaatgta gtaacaaccg ctgttccttt ccttcgtatt tcaccagatg cccgttcagg
    12121 cggtatggga gatatgggtg tggccacttc tcctgatgct tattcaggtt tgtggaatat
    12181 tggtaaagtc gcttttaacc aatcaaaagg tggaattggc gctacttaca ctccatggtt
    12241 gaaagacctg gtaaatgatg tttatcttgc tacagttgct ggatattata aatttgatga
    12301 gaatcaggct ctctctggtt ctgttcgata ttttagtctt ggtaatattg atttcactga
    12361 cattgttggt aatacatttg caaactacag gccccgtgaa tttggtattg acattggtta
    12421 ttcccgcaaa ctttctgcaa aaagcggtgt tggtatcgct ttaaaataca ttaactctaa
    12481 ccttgctggt ggtactactg taggtagcac tacatacaaa gcaggtagcg ctgttgctgc
    12541 tgacttagca tattatcatg atggtaaaaa gccatcaggt agcggttggg catggggtgc
    12601 agtattatct aacctcggtt caaaaatctc ttacactgat aatgcagatg ctaaagattt
    12661 catccctgca aaccttggat tgggtcttaa ctataccagc aagttcaatg agagtaattc
    12721 aatttctttc ggtatagaag ccaacaaatt gttggttcca acacctcctg gtgacggtgc
    12781 tacatcacaa gatatcattg actaccgcaa taaaagtgta atcggaagct ggttcagctc
    12841 tttcggtgat gctcctgacg gatttggtga agaactgaga gaattccaat tgtctctggg
    12901 tgcggaatac tggtacaaca atcaatttgc actgcgtgcc ggttattttt atgagaataa
    12961 agacaaagga aatcgccgtt attttacaac aggtttaggc cttaagtata acgtatttgg
    13021 tttcaacttc gcttacctga ttcctacagg aagtggtgta agccgcaatc ctctttcaaa
    13081 cacacttcgt ttctctgtgt tgtttgactt agatggaggt gctaaagcag cgaaataata
    13141 attcagcttc ttaatagata ctaacgagtc ccgtttcggt ttaaccgaaa cgggactcgc
    13201 ttttttatac tgtttgtatg accaggtgtg ctgaaggtat tttagaacgt aagcatggat
    13261 ttattgatat ttgtaaaaag gaatcaatat gtcttaccgg ataggttcag gagtcgattt
    13321 tcatcagctt gcagaaggaa gggagctttg gattggtggt gtaaaaattc cgcatcacaa
    13381 aggttctctt ggccatagtg atgctgatgt gttactacat gccatttgtg atgctttgtt
    13441 gggggcatta agtcttggcg atatcggtat tcattttcct aacacagatc ctgcttacaa
    13501 aggcatagat agtaagattc tattattaaa aacaattgaa ttagtgaatg gacacggtta
    13561 ttcggttgta aatgttgata gcaccctttg tctcgaagca ccaaaaataa aaccctatgt
    13621 tccgcaaatg caaaagataa tagctgatat tttgaaaata acagtaaacg atgtatctgt
    13681 gaaagcaaca acaacggaga aaatgggctt tgccggaagg gaagaagggc taatggctta
    13741 tgcttcagtg ctgcttcaaa aagcgattgt gtaagaaatg gcctgaatcc acattttcct
    13801 ttatcccgat acctatcttt gtcctatgcc agtagtggaa gtaaaaatta ttaatcaatc
    13861 acccaatccg ttaccagcat acgccacggc cggttcctcc ggaatggatg taagagctca
    13921 cttggatgtc cctgtagttc taaagccatt tgaaagatct cttttgccaa ctggtctgtt
    13981 tatagaattg cctgagaatt atgaagtgca gatacgtccc agaagcggac tggcaatcaa
    14041 acaaggtatt acctgtttga attctcccgg tactatagat gcagattacc ggggagaaat
    14101 tatgattatc ttaatcaacc tttcagggga agatcaggtc attcatcccg gcgaccggat
    14161 agcccagatg gttattcaga aagtggaaaa atgtacctgg aggagagtgg aaatactgga
    14221 agttactgaa agaaataaag gtggtttcgg tcataccgga aagcaataaa gacgttcaat
    14281 tatgattaga atttcgatag ttcttgtttc attaatctat ttgctgggct cttgcagggc
    14341 tacaaagaag attcaatcgg ctatcgttaa aaaagattcc gttgtagtag ctattccgcc
    14401 tattaataat ggcagggaag actccattgc attcataaaa gaaaatttta atcagataca
    14461 aaagaatcgg attgatttta caacattttc agcgaagatg gacgtggatt atgatgatcc
    14521 ggatggtaaa aaaatggatg taaatgcaca gatcaggatg tacaaagaca gtatcatatg
    14581 ggtttccatt actgctattt tgggtataga aggattaaga gcttatatta ctacagattc
    14641 tgtgaaagtg ctggataaac agaataaaat atatatagcc cgcagcgttg cctatttaca
    14701 ggatataact gcattacccc ttaatctttc ctctttacag gacttgctga tagggaatcc
    14761 tgtttttctt gattccaata tcatatcata tagcaaatca tccaattctg tctccctgca
    14821 tggcataagt gatttgttta aaatgctgat aacaataggc gaacctgata atttattgca
    14881 aagcagcaaa cttgatgacc tggatgagca gcgaaaccgg accagttacc tgacctattc
    14941 cgattatgag aataagaaag gagtgccttt ttcaaaaaag agaaccatta ctgtttcgga
    15001 aaagaaaaaa ctgggtatca aattgaattt caaacaatat gagtttaatg aaacgttaag
    15061 tttcccgttt aatgtaccta aaaattataa gtatgattag ccggataaaa tttattttgc
    15121 atcttgccgt tatctgaatt cactccgcac cctgtttttt aaccccctat cccatttact
    15181 atgagaaaaa aaatattagt actgttattt tttgccggat ttggcttttt agcctacgga
    15241 cagcctgctg atgataagtc tcaactggaa aaggaaagac aggagattca aagggaattg
    15301 aatgaaatac agggtatgta taacaaggta aaagggcaaa ccaaacaaac ccttggccag
    15361 ttgaatatgc tgaatagaaa aattaacctg caggagcaat atatcggtac tatcagtaaa
    15421 gaactcagat caatagatga cgatatatat ttaagtgaaa tagaaatcta tcgccttagg
    15481 aagcaactgg atacactaaa agcacaatat gccagaactg tagtgtatgc ctataaaaac
    15541 aggagtaact acgattacct taattttatt ttttcagcaa gcagcttcaa tgatgcagtg
    15601 aagagaattg cttacctgaa aagttacagg acctacaggg agaaacaggt aaagacaata
    15661 cttgaaacac aggagcttat tgctcaaagg aagaaacagc aattggtcag aaaagatcag
    15721 aagaatgtcg cattgcaatc gcagacaaag caggtgcagg aattagctgt tcaaaagaag
    15781 gagaaggatg ccgttgtttc acagttaaaa tcaaaagaga aagaattaca gggacaaatt
    15841 gcaacgaaga agaaaaggga ccgggatctg caaaatgcca taacagctat tgttcgcagg
    15901 gaaattgagg ccgcaaaagc taaagccaaa gccgaagcag aagcaaaaag aaaggctgat
    15961 gaactggctg aaaagaaaaa ccccgaaaaa gcacccgtta cggccactaa tcccacgacc
    16021 aatccaacaa catctcctgt aacaactgcg aaaaggaatg aacccgcaaa aagaccagat
    16081 agttatttgg actttacggc aaaagacgta gccctgaata gcagcttcca ggcaaataag
    16141 gcccggttgc cctggccggt agacaatggt gtggtgacac taagatttgg caataataaa
    16201 attgaaaaca ccctgctgac ctttgataat cccggtatta caattgctac accatcagct
    16261 ggggtagcag taaaagcggt atttgacggg gaggtaagcg gagtatataa ccttggtgat
    16321 ggtatggcca ttaccatacg tcatggtaag tattttacaa cgtatagtaa tttgtccggt
    16381 gtaacagtat ctaaaggggc tgcggttaaa acagggcaac aaattggaag agcaggaaaa
    16441 gatgatgatg ggaatggcgg gcagatagat tttattttga tgatagaaac ccggaatgta
    16501 gatccgttgc catggttacg ccgctgatct ttaatgatac aataaataaa taataaagcc
    16561 acgaattttc gtggctttat tatttaaaga aatttttcgt acaatttttc gtacaacggt
    16621 acgatattat gaatatcgaa tttcttagca tgttcggcgg ctctctcttt aaatcctttt
    16681 agcgtatcgt catcctgtaa aatcgagatc gcctgcttac tcatattttc cacatcaccc
    16741 acattactca tataacctgt ttctccgggg atattgattt caccaagacc acctgcgttg
    16801 gtggaaataa ccggcacccc tgctgccatt gcttctaatg cagctaaacc gaaactttca
    16861 tattctgagg taagtaaaaa taaatcaccg atagcaagaa tgtcttccat ttgttcctgc
    16921 tttccgacaa aacggatctc atcgcaaaca cccaattcac gactcaaatc ttctgcagtg
    16981 ctgcgctcgg gtccgtcacc aacaaacagg agtttagacg ggatttgctt acttacttct
    17041 gcaaatattt ttactacatc ctgcaccctt tttattttcc tgaagttgga tgcatgcaaa
    17101 agaatacgtt caccatttgg cgctattact ttgcgaaaag catctatagg cttacgattg
    17161 aagcggctta catcaacaaa gtttacaatg acttcaatat ctttttcaat ttgaaaatgt
    17221 ttataggttt catcccgcag attttcagaa acagcagtaa tgccgtcgct ttcattgatg
    17281 gaaaaagtga ctaccggagc atacatttta tctcttccga ccagggttat atctgtacca
    17341 tgcagtgtcg taataacagg aatatttttc ccttcctttt tcaggatctg ttttgccatg
    17401 aaggcggcag aagcatgcgg aatggcataa tgtacatgca gcagatcaag gtcatggttt
    17461 ttaattacat ctaccatggt gctggaaagt gcagtttcat aaggagggta atcaaacagt
    17521 ggataagtag gcacctgcac ttcatgataa aatatgttag gcataaattc actcaacctt
    17581 acaggttgct ggtaggtaat aaaatgaacc atgtggcctt tttgagccag ggctttgcca
    17641 agttctgtgg ccagtactcc tgaaccacca aacgtgggat aacaaacgat accaattttc
    17701 ataatatgag attctaaata gaactaaggt aacagtttta accggggttt agtttgccgc
    17761 ttatcttaaa taattatctt catttttcga tttcttttaa ctttagcaaa attcaggtac
    17821 atgcgaaaat cttccctttt tattgtcttt acacttttta cccgggctat tttgggacag
    17881 caatcagact cggcatttat taagcagatt tccaatgaaa tattaacaaa tggaaaggcc
    17941 tatgaaaacc tgcggtattt aacaaaaaag atcggtgcaa gactggcagg ctcacccggt
    18001 atggtaaaat cagaacaatg gggcctgaag gtaatgcagg aaagcggtgc agacaaagca
    18061 tggctgcagg agtgtatggt accgcattgg gtaagagggg gaaaagacgt agcctctgct
    18121 ttttatcttc aggcttcaaa acagggtata aatgtgagca ggatacaaaa ggagcttgat
    18181 gtggtagcct tgggtaattc tatcggaacc ggaaagaatg cagtttctgc tgaagtaatc
    18241 gtagtgaatt cttttaatga gcttgaaagg agaagtgctg aaataaaagg gaaaattgtt
    18301 ttttataatt ataaatttaa tgacgcctat gtcaatactt ttctttctta ccgcgatgca
    18361 ggacaatacc ggggacaagg gccaagccgt gctgccaggt atggggcaaa agccgttata
    18421 atcaggagta tgagtcatgc aactgataat aatccacata caggtgcaac aagatatgat
    18481 agcaattatg caaaaattcc agcagttgcc attggcctcc gggatgcgga ttggttaagt
    18541 gaacaggtta aaaagggcgg agtaagtgca acaataaaaa ccaatggtca ttttctgcct
    18601 gatacaatcg ggcataatgt aattggtgag ttgaaaggga ctgaatttcc tgatcagata
    18661 ataaccattg gggggcatct ggatagctgg gataattgtg aaggcgctca cgatgacggg
    18721 gctggttgtg tacaaactat tgaaatactc agggcattta atgcaatcgg gtataagcca
    18781 aaaaggacaa ttcgctttgt tttatttgcc aatgaagaaa atggattaag aggtggaaat
    18841 aaatatgcag aagaagcaaa agccaacaat gaaaagcata ttctggctat ggaaagtgat
    18901 gccggtggtt ttacacccag ggcattaggc ttttcaggaa ctgaagaaca gtttcagaaa
    18961 tttttatcat ggagggaatt aattgcacct tatggttgca gtgagtttgt aagtggtggc
    19021 ggtggtgcag atatcggacc gttgagcagg gcattaaaaa cacccacagc cagtctgaat
    19081 cctgatacac aacgctactt tgatattcac catgcccgga gtgatgtatt tgaagcagtg
    19141 aataaaaggg aattggaatt aggagccgtt aatatggctg cattgattta tttagtagat
    19201 aaatatggat tgtgatatgg agaactatta tgaagtaagt aatggagaat atttaataac
    19261 tactaatcct tctttactta gtgtggatat tattcaccat tatctttctg aagaatcata
    19321 ctgggcaaag cacataccga gggtggtggt ggagaaatca attaccaact cgttgtgctt
    19381 tggtttatat cgacagagtg aacaaattgg atttgccagg ctagttactg ataaagccac
    19441 atttgcttat ttggcagatg tgttcatttt agaaaagtac agaggcaagg gcttatctaa
    19501 atggctgata gaagtgattc aatttcatcc agaattacaa ggtttgcgga gatggatgct
    19561 gggaacaaga gatgcacatg gactgtatgc acagtttggt tggtcagaaa tttctgaaga
    19621 aacaagaaag atatttatgc aacggcattt ccctgatgtg tatgctgtta gctgaccaat
    19681 tttgtaatag ctgcctttac attttcaaaa ggaaaagaag caataatttt ttccagctgt
    19741 tgcttgattt cagcagtacc aagattccca atacttcctt catgggtatg gtttacagaa
    19801 gtagtgtaag tgaatttatc agcttcaata tctgagccta cttttttata cgcatcccgg
    19861 aaaggagttc cgctatttac taatttattt acctcctcta cactgaacag gaatttgtat
    19921 ttatcgtcag ccagtatgtt ttctttaacc tgaatatttg ataacatcag cccggccatt
    19981 tcaatacaat ttttcaatgt tttgaaggca gggaacaaat gttctttcaa taactgcaaa
    20041 tccctgtgat agcccgatgg caggttagta gtcatcagca ttatttcatt tggcaatgct
    20101 tttatttgat tgcaataaga acggattaat tcaaatacat caggattctt tttatgtggc
    20161 ataatgctac tgccagttgt taattctaca gggaaggaaa taaacccaaa attctgattc
    20221 aagtataagc aggcatccat gcttagttta gctaatgtat cggctacatt cgccagtgat
    20281 tgtgcaacaa tacgttcagc ttttcctctt cccatttggg catacactac attataattc
    20341 aaatcagcaa agccaagcag ctttgtagtt agcgttctgt tgataggaaa agaagagcca
    20401 taaccggctg cagaacctaa cggatttttg ttcaccacat cataagcggc tttgatagtt
    20461 agcatatcat ccaccaggct ttctgcataa gccccaaacc aaaggccaaa tgaagaaggc
    20521 atggctaatt gaagatgcgt ataaccgggt aataagtgat ccttatattt ttcactttgt
    20581 gattgcagta attcaaaaaa ggactgaatg gtttttacca attcttccag ttcattcctg
    20641 agaaacaatt ttatatcgac aagcacctgg tcattgcggc tgcgggcaga atgtattttt
    20701 ttacctatat caccaagctt ctgtgtaagt agcagctcga cttgcgaatg aatatcctct
    20761 acatcatcct gcaaaatgaa ttcacctttt ttaatctgtc ggaaaacgtt ctttaattct
    20821 ttctgtaatt gtgataattc atctttggta agtaatccaa ctgattccag catttctata
    20881 tgtgccagtg aacccagcac atcaaaagca gctaaataca tatccatttc acggtctttc
    20941 ccgacagtga agatttcgac ttcctttaat gaggctttat ctttttgcca cagtttcata
    21001 caatctgatt taaaagctgg atatataatt ctatgccttt ttttatttca tcaatataaa
    21061 tatactcatt ggcggtatga cttctggcag aatctccggg gcccattttc aatgcaggaa
    21121 aaggcattaa tgccttatca gaagtagtag gagagccata gtaagcactg ccaagattta
    21181 atcctgcctt cactaaagga tggtcaaggg caattgaagt agaacgtagt cttgtacttc
    21241 tgggcatcac ctcacactgt acattggctt taataatatc gatcacttct tcaaaactat
    21301 ataattcgtt tattcttgta tccactacaa atttgcacag agctggaaca acattgtgtg
    21361 ccttattttc tgtttcaatt acagtcacac tcattttgga agctcccagc agttctgaaa
    21421 ccctattaaa ctgataggta ctgaaccact caatatcttt cattgcttta tagatggcgt
    21481 tctctccttc atttcttgca gcatgtcctg ctttgccatg tgctatacag tccagcacca
    21541 tcaggcctct ttcggctact gccatctgca tttgcgtcgg ttctccaaca atggcgaaat
    21601 caatttttgg caggtaaggt aatgtgtatt ctataccacc gctgccactt atttcttctt
    21661 ccgcagtcgc agcaaggata aggttatatt tcaggtcctg tttgttgtaa aaatgtagaa
    21721 agactgcaat tagggaaact aaacagccgc cggcatcatt acttcccagg ccaaagagtt
    21781 ttccttctac aatagatgga taaaaggggt ctttgctgta ttgcggattg ggttttactg
    21841 tatcatgatg agaattgagg agtatagtag actttttttc atcaaaatgt ttgttcaaag
    21901 caaaaacatt gttgccaacc cttgtatgta caatatcctt attccctaaa aaccggcaaa
    21961 ggatacctgc cgtctggtct tcttccttgc tgaatgaagg ggtagcgatc aattctttca
    22021 gcaattcaac cgcttggtct tgcaatatgg aaatttcttt actcatttat tatagtcgtt
    22081 cctgaagaac cattaatcag tcctttcagg ttttctgctt tgccgatgat tacttttccg
    22141 acgccacttt tcagggcagc gaatgcatta tccatttttg gtatcatacc ggcaaatatt
    22201 ttatttttcg acttcaattg aaggtaagaa gatggtttta tttccggaat gactgttgtg
    22261 tcatcatttg catccagcag cacaccactc ttctcaaagg aatagataag ctgcacatta
    22321 tacaaatcac tcatgctcct ggcaatctcc tgggcaatag tgtcggcatt cgtattcagg
    22381 agttgtcctt gctggtcatg tgtgatgggt gcaaacacaa cagcaatatt cttctccagc
    22441 aggttattga taagattagt attgattgca tccacatcac ccacaaaacc atagtcgaga
    22501 acaggatgtt gccttttatg agccagaatg gtgtctccat cggcgccgca gagacctatg
    22561 gcattacaat tattggcctg cagcacagct actatatttt tattgataaa accagcgtaa
    22621 accattgtaa caatcttcag tgtttccgca tcagtgattc ttcttccgtc cactaattgc
    22681 tgttcaatac ctagcttttc agcaagtctt gtcgcaagct tacctccgcc atgaaccagt
    22741 atcttctttt cttcaatgga cgcaaagtct gccaggaatg aagccaattt tacttcatca
    22801 tcaatgatat tgccaccaat ttttatgaca tataatttat ccattggttt ttaggatttc
    22861 tgcgagtacg gactgggcag cccagatcct gttcgcagcc tcttgtgtta caatactgtt
    22921 aggcccgtcc agtatttcat cacttagttc tacattcctt cttacaggaa ggcaatgcat
    22981 tattttagca tcattggtca attccagttt tttgttgttc agcatccact tagggtcgct
    23041 ttcataaatt tttccgtagt cgttgaaagt actccagttc tttacataca caaagtcagc
    23101 attttttaat gcatcattct ggttatatgt gatgatagct cctttagtaa attcttcact
    23161 caattcataa tcctctggat gggtgataac aaaatctgct ttaccccagc tattaaccca
    23221 ttgggaaaaa ctattggcta cacactgggg caaaggctta acatggggtg cccagcttaa
    23281 aacaatttta ggttttcttg tttcgttgaa agattcagtg attgtaataa tgtcagtcag
    23341 gctttgtaag gggtgtagtg tagcactttc taaactcaca acagggatcc ctgcatattt
    23401 aataaactgc tttatgtaca attcactgta atcatcatcc cggtttttca gcgatggaaa
    23461 tgtcctgatt gccagtatat caaaatattt tccaaagatc ggagctgcat ctttcacatg
    23521 ttcaaccgat gtgccactca tgatggcttc ctcttcaaat tccagcatcc agccttcgtt
    23581 gccaacatta aagatgatgg cttccattcc taagttttgt gcagcaatct gagtactcaa
    23641 ccttgttcgc atactggggt tcaaaaatag acaacctatc cgtttgttaa ttccgagtgt
    23701 tttatcagcg aaagggtttg ctttatacgc taatgcctgt tgtactaaag catcaatatt
    23761 actgacatct tgaactgaaa caaaatttct cattttaact gactgggttt gaagccggaa
    23821 ctaagttttg aatttcttct tttatcgaat caataaattg ttctgcatct cttttcttta
    23881 aagctaatga gggcaacaac cgaataacat ttggcttagc ttcaccggtg aatattttct
    23941 ggttccagag gagatttttc ttcaggtctt ttagttcttc cggtacatca aaaccaatca
    24001 ttagtccccg tcctcttaca ttttttagtt gtggaataac ttccagctga tctttcagga
    24061 atttgcctgt cattactgca ttacccatca atttttcttc ttctattgtt tcaagaactg
    24121 ccagcgcagc agcacaagcc aggtgattcc ccccaaatgt agtacccagt tgaaaatgtt
    24181 ttggtttaat atgtggggca attatcagtc ctgctacggg aaacccgttt cccataccct
    24241 ttgccattgt ataaatatct gcatttaccc cggcaaaatc gtggctgaaa aatttcccac
    24301 ttctgccata accgcattgc acactatcag caatataaag agccccatat tcatcacaaa
    24361 gggaacgtat ttttctcagg aaggaatcgt cagctatatt aatccctcca actccctgaa
    24421 tcccttcgat aattacagat gaaatttcct ttccgttttc attaaaaaat atttctaagg
    24481 cttcttcatc attgaaagga agaaagctga cattcccggt ttcattaacc ggggcaatat
    24541 agtttttatt gtctgttact gagactgcta atgatgttct gccgtgaaaa gctctgctga
    24601 aagcgactat tttttttctt ccattatgaa agcttgccag tttcagtgca ttttcattgg
    24661 cctcggcccc gctattgcag aggaatagct gataatctgg tttgccggaa actttaccca
    24721 gcttttcagc cagatgttgt tgtaatggaa ttcgtattga attggaatag aaagcaattt
    24781 tttccaattg ctcctcaatg cgctgaaccc aatgcggatg ggtgtgacca atactgatga
    24841 cagcatgacc gccatacaga tcaaggtatt gctcgccatt gtcatcccat acataagagc
    24901 ctttggcttt tacaatagtg atctcattta tcgggtaaac gtcaaaaagg ttcatttgta
    24961 taatttagaa ataattggct ttaagtttta atccggttgt ttcatccagg ccgaatatca
    25021 agttcatatt ctgtacagcc tgcccactgg cgccttttag taagttatct atagcagagt
    25081 gtactacaag tttactgcct gccttctcca attgtatcac tgctttattg gtatttacta
    25141 cctgcttcaa aaaaatcggt tctttgctca cataggtaaa agggtgggca gcataaaaag
    25201 ttgagtataa taaattcaat tcttcaatgc ttaaatcaca atttgtggta gaactgataa
    25261 aaattcc
//
LOCUS       DAORTI010000040        25013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1545_length_25013_cov_7.837848, whole genome shotgun sequence.
ACCESSION   DAORTI010000040 DAORTI010000000
VERSION     DAORTI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 25013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25013
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_25013_cov_7.837848"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="PLO70_07210"
     CDS             <1..1429
                     /locus_tag="PLO70_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HQZ74288.1"
                     /translation="KKLIQTPNIDRLAKEGIKFNNAFVGNSICGPARATLLTGQHSHK
                     NGVIDNRKRFDSSRMSMPKLLQAAGYQTAVIGKWHLHSYPAGFDYWKILPGQGLYVNP
                     RLIEMTGDTITQQGYATDVITDETIKWLEQRDKSKPFVLLLHHKAPHRYFFPDLKYLE
                     EFHTKTFPEPATLYADTMNRSSAWHIQTMSILRDMQLCSDLKVDPAYVMDIPEYKPDS
                     TQIAYYKAIMNRVPEKQRIRYKEIYAERGKLLRDLRPTGKELLKYKYQWYIQDFLACV
                     ASVDENVGRILDYLDQKKLTNSTAVLYTSDQGFFLGENGWFDKRFAYDVSMQTPLLMR
                     WPGQIKPGSVTNSMVQNIDYAPTILDIAGVPVPGWMQGLSLKHLVTNKKKTLNRNYLY
                     YHYYEFVRDHTVIPHLAVRGTRYKLIYFYTVNEWELYDLKTDPQEQKNLIKNPAYQKT
                     VQQMKAELLKLRDQYDDHEKAGELN"
     gene            1440..2600
                     /locus_tag="PLO70_07215"
     CDS             1440..2600
                     /locus_tag="PLO70_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQZ74289.1"
                     /translation="MKKVLAGVDQLLVQENKFQDYRLALVTNNVASISTGESSRTALL
                     KAGFKIIKLFSPEHGLSAKGEDGAFQRNSFDTLTQLPVISLYGDHLKPAPEELYDIDM
                     VLFDIPDIGCRFYTYLWTMTYVMEACAACKKPLIILDRPNPLGGDLERAEGPLLDEER
                     CRSFIGRWNIPVRHSCTMGELATYFAAKRIMNVDLTVIKVQNWNRKQTVLEADWLFTP
                     TSPAILDAETALLYAGMGLLEGINVNEGRGTGSPFKVCGAPWINGEQLNRVFMDTRLP
                     GIKSTAITYIPTHGLYAGEKCQGLRFFLTDHHLFTPVKAGLQLLQCIISLYPGQCKER
                     LYPTVANPTGKGHLDKLIGIYRSFEKIGEGEIPSTILQQKDWKEIISPYLLY"
     gene            complement(2594..3406)
                     /locus_tag="PLO70_07220"
     CDS             complement(2594..3406)
                     /locus_tag="PLO70_07220"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQZ74290.1"
                     /translation="MKYKFRVSLFLGIVIIIYSCSQAKYAATNKSYKKQVKKYAKMLA
                     EYPVKDSFTTAAEWVGTTNFSMRRPNYVIIHHTAQNSCEQTLKTFTLPRTQVSSHYVI
                     CKDGTVHHMLNDLLRGHHAGVSKWGNATDLNSSSIGIEIDNNGFETFTEPQINSLMEV
                     LGKLKGAYNIPAANFIGHADIAPGRKVDPNRNFPWQKLAQKGFGLWYDTTNISVPKDF
                     NPVQALRIIGYDVKNEKNAIQSFKIHFVQSDTTKIINESDKKILLDLMKKYQ"
     gene            complement(3446..4660)
                     /locus_tag="PLO70_07225"
     CDS             complement(3446..4660)
                     /locus_tag="PLO70_07225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQZ74291.1"
                     /translation="MRFTEFNFHPNLLEGIEASNYETATPVQEQVIPPIMAGKDVIAS
                     AQTGTGKTAAFLLPVMNRLLQNHVDGQVGALIIVPTRELAIQISQHMEGLSYFTHLSS
                     IAIYGGNDAQNFVAEKKALQMGADIIVCTPGRMIAHLNMGYVNMKGLQFLVLDEADRM
                     LDMGFQDDINKIIKALPMKRQNLLFSATMPEKIRQLAKKILHEPVEINIAISKPPEKI
                     IQKAFVVYEPQKLPLIKKLLKEIPYKNALIFCSRKQTVKQLTRELKHGGFNISEIHSD
                     LEQSERESVLSAFTAGRIPVLVATDILSRGIDIDTIDVVINYDVPGDGEDYVHRIGRT
                     ARAEADGAAFTLIGEKEQNKFAVIEQLLGKEVEKAVVPAELGEAPSYHPRTRAGHSGP
                     KRKFYKSGPKRR"
     gene            4859..5446
                     /locus_tag="PLO70_07230"
     CDS             4859..5446
                     /locus_tag="PLO70_07230"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HQZ74292.1"
                     /translation="MKYSLLLGAIIISMAAYSQAWTKQYDHVDECTCGLSLVGKDDKH
                     GFVDKDGKLVVPLIYEDALTYSEGFAAVKLNGKWGYLDSTGKVLVEPKYTEAMSFHNG
                     FAVVSNGNDYGFIDTSGKIGIQMIYENASQFSEGLAAVNKKGMWGYIDTKGREVIACK
                     YGFAGTFGDGSARVMQNSKWIIIDRTGKKIADSER"
     gene            complement(5504..7897)
                     /locus_tag="PLO70_07235"
     CDS             complement(5504..7897)
                     /locus_tag="PLO70_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74293.1"
                     /translation="MMEKRADILFVFLLICVGLQAQNIDATIARYANEYGQERTYLQY
                     DKTAYVPGETIWFKAYLMKAISPADDSKTFYTDWTDEAGNLLLHGVSPVVDATSNGQF
                     DIPADYTGTFIHVRAYTKWMLNFDSAFLYEKDIRIITKNGNTPAAKIPVVPTLQFFPE
                     GGDAVAGLSNKIAFKVTDQWGKPVKIKGLVQSSIGEEIDSLRIIHDGMGYFFLFPRLG
                     ESYTAKWKDENGTEYVTALPAIKPTGVSLQVTLSGVKRTFAVTAEQNLAKDLGTLHIV
                     GTINQYQVFKLTKDMSAGFVKGIIPTQDLPSGILTITVFDRQWNPLAERITYINNDEY
                     RFNAEMTVELWGLNKRARNEILITVPDSLEASFAVSVTDAAIGTDSSENIISHLLLTG
                     DIKGQVFNPCYYFSGNNDTIANRLDLVMLTHGWRRFKWEDVVKGKLPEIMYPRDSTYL
                     TLSGKVYGATNAQLRDAATIILIVNQKSQADGNKMVMLPVAPDGTFNDPSYFLFDTAQ
                     IYYKLSTGIKDGSATFMESRLPALRYRMPATGIFYNRLGDTTGYSRHFRLSDETLRLL
                     QESEGKVLENVIVNTKTKSSVEIMDEKYTSSGLFSGGDSYKFDLVNDKFRFAYQNIFQ
                     YLQGKVAGLQINTSSNPPSLMWRGGPPQLFLDEVPLDADFVSSIAVSDIAYVKVFRPP
                     FFAGSGTSTNGAIAIYTRRGNDGQAEPGKGLASNKVSGYTPIRQFYSPNYSSFNAANE
                     KKDLRTTLYWNPQVVTTAAKNKMKLTFYNNDVSKSFRVIIEGITKDGQLVHLEQIME"
     gene            complement(7995..11351)
                     /locus_tag="PLO70_07240"
     CDS             complement(7995..11351)
                     /locus_tag="PLO70_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQZ74294.1"
                     /translation="MSYTPSNKVRIVTAASLFDGHDAAINIMRRIMQSKGAEIIHLGH
                     NRSVKEIVETAIEEDVQGIAITSYQGGHVEFFKYMKDLLDENGCGHIKIFGGGGGTIL
                     PDEIDELHKYGIARIYSPDDGRHMGLEGMIEDVIKQCDIHLNGNADYKTALKLEEAKD
                     IRVIARKITNAENGVVLDAGSKELGVGRIPVLGITGTGGAGKSSVTDEIVRRFLNNFK
                     EKTIAVISVDPSKKKTGGALLGDRIRMNAINHPRAYMRSLATRDDNTALSSAMQDAID
                     ICKAAGFDFIILESAGVGQSDASILDYCDVSMYVMTPEYGAASQLEKINMLDYADLVC
                     INKFDKAGALDALADVRKQYKRNHQLFTAIDEELPIIGTMASKFNDDGVNHLFELLLK
                     KIDAKTNTSFGKYHFAETAKVSQAVIPPNRVRYLSEIAENNRSYDQLVQEQSAIASKL
                     YQLNGSLETLKESADKSLLEIIQKQINFYTEQLTPVARKLITNWPNKTAQYQKDFYEY
                     KVRDKVIKQEMFSKSLSGTRIPKVVLPKYKDWGDLLTWQAQENVPGSFPFTAGVFPLK
                     REGEDPTRMFAGEGGPERTNKRFHYVSVDQPAKRLSTAFDSVTLYGEDPDHRPDIYGK
                     VGNSGVSIATVDDAKKLYSGFDLCDPRTSVSMTINGPAPMLLAFFMNTAIDQQCEKKI
                     TELGLWDKVRKISADKFKHFPPPKYHNSSSPGELPEGNDGLGLKLLGLSGEEVLPKEI
                     YDHCKQTALQQVRGTVQADILKEDQAQNTCIFSTEFALKLMGDVQEYFINNKVRNFYS
                     VSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMNIDDFAPNLSFFFSNGMDPE
                     YSVIGRVSRRIWAKAMKYKYKANKRSQMMKYHIQTSGRSLHAQEIDFNDIRTTLQALY
                     AIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGTAKTENFIQGSFAIEEL
                     TDLVEEAVLAEFDRITERGGVLGAMERMYQRNKIQEESLFYEHQKHSGELPLIGVNTF
                     LNKKGSPTILPTEVIRSTTEEKEQQIQTLRSFWKRNENKSADMLQRLKAVAINNGNLF
                     EELMETVKYCSLGQITHALYEVGGQYRRNM"
     gene            11445..12911
                     /locus_tag="PLO70_07245"
     CDS             11445..12911
                     /locus_tag="PLO70_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQZ74295.1"
                     /translation="MQHLIESITSLYKKWKGADPVTVDLLPQSGSERRYFRLHSKDET
                     VIGTYGANIKENETFNYFSKHFKEKNLAVPEILAISDDGMFYLQEDFGDISLLNHLEA
                     KGFTEEVYELFKKSLEALAQLQVKGDKGLDYNNCLTNTEFGKQAIMADLLYFKYYFLD
                     ALRKPYDKQKLIDDFEALSNYLTHTEYKYFMFRDFQSRNIMVMEDNSVHFIDYQGGMK
                     GAPQYDVASMLWQARANLPDKWKYDLLEDYIDAFENTIGKSVNRNIFQSQYNGYVLIR
                     LLQVLGAYGFRGLFERKAQFLTSIPLALRNLRDFFQHQSIGISVPEFRKVLDLCVADE
                     IIQQFTPTQATENTPLLIKIHSFSFRKELPRDNSGNGGGFIFDCRGILNPGRIESMKT
                     QTGRDKEVKDFLEQQTKMPEFLNSIFDIVDITVEEYIKRDFESLMISFGCTGGQHRSV
                     YAADAMARHLKNKFKVRIELLHIEQEAKNWINPLPEKK"
     gene            12911..13648
                     /locus_tag="PLO70_07250"
     CDS             12911..13648
                     /locus_tag="PLO70_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQZ74296.1"
                     /translation="MKAMIFSAGLGTRFKPWTDKHPKALAVINGKSLLQRNIEYLQQN
                     GIRDVIVNVHHFAEQIEEAIVSNDGWGSNVLVSDERDEVLETGGGLLKAEELFTTGEK
                     FITCNADFLTDLNIQNLIHYHNEKKALITFGVTNRKSSRVLLFDDHMRLTGWRNTVTG
                     EERIAIPPPAEGVRGRPLAYSCVVVFEYRIFELMRQYGFIGKFSLIDVYLKLAPEHLI
                     MGYEHSGDRLVDVGKPESVEIAESLFP"
     gene            13723..15678
                     /locus_tag="PLO70_07255"
     CDS             13723..15678
                     /locus_tag="PLO70_07255"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQZ74297.1"
                     /translation="MKKFVSFICSLFLLVSLSAQSKYDHKEAFNPQFYPYPGNDYRSA
                     SGEPGPKYWQNKADYSINCKLDTATHTVTGDVAITYTNNSPDNLRFLWLQMDQNIYKK
                     DSRGSATTTQTGGRWANAGFTDGYTITSVELESAGVTYAPNKTITTDTRMQIILKDQL
                     EKGEKVKIKISYNFTVPEYGTDRMGRLKTKNGIIYEVAQWFPRMCVYDDIQGWNTLPY
                     VGAGEFYLEYGNVDFSITAPSDLIIVGSGELINPQDCYTAEQMKRWETAAKSDKTVTI
                     RSDKEITDKNSRPKTANTTWRFSIQNTRDVAWSASKAFVQDAARINLKSGKKCMAISV
                     YPAESIVKNGWQRSTEFTKASIEHYSGKWFEYPYPAATNVAGIVGGMEYPGIVFCSSE
                     ASGTGLWGVTDHEFGHTWFPMIVGSNERKYAWMDEGFNTFINDGSTESFNNGEFKENS
                     FFGDPNSSFMVKYTFGDKMDGLFNTPDVIQQDNLGIAAYMKPAQMLTALRDVVLGKER
                     FDAAFAEYIRRWAFKHPTPWDFFHTMENVSGEDLSWFWRAWVLNTWKLDQTVKAVAYV
                     DEKPEKGVEITIENLEKMVMPVAVLVKESNGKEHKINLPVEIWQRGAEWRFNVPTTSE
                     IKEVILDPDKKLPEWDRTNNSWKKKGF"
     gene            15730..16203
                     /locus_tag="PLO70_07260"
     CDS             15730..16203
                     /locus_tag="PLO70_07260"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HQZ74298.1"
                     /translation="MKKMILLIIISSTIFRVTAQVNEMKFSELFLLKGTWSMKTTKGM
                     LYENWVINNDSTMTGKSYRLNNTDTILLETVGLVMRGSQILYIASAEGQNNQQAVAFK
                     LVNRDNDTFIFENPEHDFPQRVIYELPKHEKLHAWIEGTINGQSRRSDYHYKKII"
     gene            complement(16209..17351)
                     /locus_tag="PLO70_07265"
     CDS             complement(16209..17351)
                     /locus_tag="PLO70_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ74299.1"
                     /translation="MRNFLRIILYKPILKIAAKFSSKPKRERIFEALGKLYTEIHTNP
                     GKKGLVIPFVESTGKFIIFSDQHKGRRNGADDFTPCEANYLHALEYYNGHDFCFINLG
                     DSEELWENNIWQVKKKNKEVFDTEKKFIRRSKYIKIFGNHDLFWDNDPLAWWHLKDIY
                     GEKIKVYEGVILELLARGQEPGMNTADAELPISGSPLTIFLTHGHQGDAQSDGNWFSK
                     FFVANIWAPLQAFLRINPNTPAYDAEKKTLHNNIMYEWSSEQKNVVLITGHTHQPVFG
                     SLTYLERLYKNFQQAIHTNDKNREEEIRREIRRRENDFTAVSVDYMKMKPSYFNSGCC
                     CFDDGDITGIEIADGFIRLVKWSGKNALTERVLLEEVSLEQLMGQL"
     gene            complement(17555..22111)
                     /locus_tag="PLO70_07270"
     CDS             complement(17555..22111)
                     /locus_tag="PLO70_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74300.1"
                     /translation="MKKFYSLPLLSLLLATGSLLLSVGCGTKNKVSDQEEGGEYSGPE
                     QRAAWEIERTKDPATGKVPWNKLLLAIEQTKQAKEFSRNNAIGALSWTERGPDSDVVG
                     PSNGNSRANGGVTSGRIRAVMIDSLDPTKKTVFVGGVDGGLWKTTDITTSPANWIPVT
                     DFLSNLAIAAICQDPRPGFQNTMYFCTGESYFNFDAVQGVGVFKSTDGGASWNFLSST
                     SAYTACTRILCDFQGNVYLATRGNGLLRSVDGGSSWTDITPSGVGNRICDLEISSTAT
                     AARLHMVTGINSAQFYRYTDVPATVTTGAGWNAPVTAFPSFNNRAEIAVSGDILYSLP
                     CNNSGQVTNVYKSTDGGVNWVATAGIPPTTVTGAAFTNTQGWYDLSVRINPANPDECI
                     IGGIDCAKTINGGDTWARISGWVGTSGQYVHADQHDVQWWDGGNKLLFASDGGMHFSA
                     DKGITIRDRNVGLRLKQFYSVAIHPTTTNYFIGGTQDNGVHQLANAGLGASVEVTGGD
                     GGFVAIDQNEPQFQFGSYVNNVYRRSTNGGANWSTPVNITSSGRFINPWDYDNNANII
                     YACNSGGTYFRWNDPQTGSTTSIISIGEFAGQNVSAVHASPYTANRVYFGTGNGRVVR
                     VDDANGATPIEANITPAGATGYVNCVVTGSSDQNLIACYSNYNVMNVWVSTNGGTSWT
                     GIDGNLPNMPVRWALFHPDDNAKAFIATETGVWETDLINGSGTVWNANATFPNVRTDM
                     IKYRSSDRTIVAGTHGRGIWTATVSSALPSGFSFTSPAPASAACPAPASMAISLGTIS
                     AGGFSNPITLSATGNPVGTTVSFSPNPVNPGSSSTITLNGTNTLASGSYVITVTGTAT
                     GATNQTRDLTYTITAGSGPAITTQPLSQTVCEGANVTFSVVATGTYQWQVSTNGGGTW
                     SNVSGQTTSSYTITGVTTALNNNQYRCVVSNQCGSTNSNAAVLTVNSIVSITAQPTDL
                     TLCAGSGATFCVTATGAGLTYQWEISPAGCGGPWTNIAGATSSCYTIPSVTAGMNNTG
                     YRCKVSGTCGPTTITSGCALLTVATAVSVTTQPVNQTICDGASTSFTVAGSGTGIIYQ
                     WQVNTGSGFTDISNGGVYTGATAATLVITSATTAMSGYQYRCQLSNPSCTTPGVSNAA
                     TLTVNALPAITGSPVNQTICVGGNTSFSVTATGTGINYQWQVNTGSGFANINNGGEYA
                     GATTSILTITGATIGLNGYQYRCVVTGTCSPAANSAAATLTVHAPVVVTSSPANAEVC
                     SGSDASFIVSGNSVPSIIYQWQVSTDGGTSWTNIAGANAATFTATGVIAVMNNNRYRC
                     LLSNVTCATPVASNAAILTVRQLPAVGLTAAPLTSLLPGQTTTLTATPTSSTGGTLVT
                     TWLLNNNPIAVSGNTLPVTVANPGSYQVRIQETWPSSIFCSSLSQVVTIDASVSSKLF
                     IFPSPNDGNFTVSYYNNGGNSTQRRIIIFDSKGSTVFDRKFPVAGSYTLIPVNLEKAN
                     RGIYYVVVGDVNGMKLAEGRVHVR"
     gene            complement(22233..22616)
                     /locus_tag="PLO70_07275"
     CDS             complement(22233..22616)
                     /locus_tag="PLO70_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQZ74301.1"
                     /translation="MGKSIIKTTSAPAPIGPYNQAVLTGNMLFISGQICIDPTTGDLK
                     NKDIQEETHQVMHNLKAILQEAGMDFGHVVKTTIFITDMQQFGVINEAYGKYFKGDFP
                     ARETVQVSALPKFVNVEISMIAVNS"
     gene            22685..23302
                     /locus_tag="PLO70_07280"
     CDS             22685..23302
                     /locus_tag="PLO70_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQZ74302.1"
                     /translation="MTIAVVASEAFKEELLAQGLKSDIKAEWLNKPANVPGTACYIDL
                     LFSSGHSEELLKLQPALIIVNSVPDTTASLPENFIRINGWNTFLKRPIVEAACSDIPA
                     RIKAEEIFAAFNKTIEWVPDIPGFITPRVIATVINEAYFTLEEKVSSKEEIDIAMKLG
                     TNYPYGPFEWSKKIGLKKIFDLLTLLAKTNARYEPSPLLKKEALL"
     gene            23303..23971
                     /gene="tsaB"
                     /locus_tag="PLO70_07285"
     CDS             23303..23971
                     /gene="tsaB"
                     /locus_tag="PLO70_07285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQZ74303.1"
                     /translation="MALILNIDTSLDTASVCLGNNDGVLQVAINDNQKDHAAWIHTAI
                     NKLLKETGFPLKSLEAIAVSIGPGSYTGLRIGLSSAKGLCYALDIPLITISTLKMIAS
                     AVKQKATDLICPMVDARRLEVFTGLYNKDLLEISAARALIIDETSFDTFLSSHKILFC
                     GNGSKKLQNILSSPNASFIETIPNASELSDLSQNCYINKQFANLAYSEPLYIKEFYTP
                     GRKD"
     gene            24072..24410
                     /locus_tag="PLO70_07290"
     CDS             24072..24410
                     /locus_tag="PLO70_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQZ74304.1"
                     /translation="MSNYSMTLTDPKENGVLLKVDLLEVKKAALVLRALNHKLRQQIL
                     KLIDESGKMTVTELYVKLRLEQSVASQHLAILRKAGFVKTERDGKFIYYSVNINRIHE
                     LNRFVGELLN"
     gene            24524..>25013
                     /locus_tag="PLO70_07295"
     CDS             24524..>25013
                     /locus_tag="PLO70_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ74305.1"
                     /translation="MFIKQLYTGCLSEAAYYIESNGEAAVIDPLRDIDAYLELATERK
                     ASIKYIFETHFHADFVSGHLDLGKATGAPIVYGPGTLTKFPVHVARDGETFTIGKLAL
                     EVLHTPGHTLESTCFLLKDENGKDHCVFTGDTLFVGDVGRPDLAQKGTEITMADLAGM
                     MYD"
BASE COUNT         7417 a         5347 c         4972 g         7277 t
ORIGIN      
        1 taaaaaactg atccagacac cgaatatcga caggctggcc aaagagggaa taaaattcaa
       61 taatgcattt gtaggaaatt cgatctgtgg cccggcaagg gctactttgc tcactggcca
      121 gcattcacac aaaaatggtg tgattgacaa ccgtaaaaga tttgattcgt cacggatgag
      181 tatgccgaaa ttattacagg cagcaggata ccagacagcc gttatcggta aatggcattt
      241 acattcctat cctgccggtt ttgattactg gaaaatatta cccggccagg gattgtatgt
      301 taatccacga ttgattgaaa tgaccggcga caccattacc caacaaggct atgcaactga
      361 tgttataacc gatgaaacga ttaaatggct ggagcagaga gacaaatcaa aaccatttgt
      421 attgctgctg caccataagg ctcctcaccg ttatttcttc cccgatttaa aatacctcga
      481 agagtttcat accaaaacat ttcccgaacc tgccactctg tatgctgata caatgaaccg
      541 tagcagtgcc tggcatatac aaaccatgag catactcaga gatatgcaat tatgcagtga
      601 cctgaaagtt gatccggcct atgtcatgga tattccggaa tacaaaccgg attcaacaca
      661 aatagcctat tataaggcca tcatgaaccg ggttcctgaa aaacaaagaa tccgctataa
      721 agaaatctat gcggaaagag gaaaattgct aagagactta agacctacgg gaaaagaact
      781 attaaaatat aaataccagt ggtacatcca ggattttctg gcctgtgtgg catcagttga
      841 tgaaaatgta ggacgcattt tagattatct tgatcagaaa aaactcacta acagtactgc
      901 cgtattatac acaagcgatc agggcttttt ccttggtgaa aatggctggt tcgataagcg
      961 gtttgcttac gatgtttcca tgcagacccc tttattgatg agatggccgg gacaaatcaa
     1021 acccggatca gtaacgaata gtatggtgca aaacatagat tatgcaccca ccattctgga
     1081 tatagccggt gtaccggttc ccggttggat gcagggattg agtttaaaac acctggtaac
     1141 aaacaagaaa aaaacattga acaggaatta cctgtattat cattattatg aatttgtaag
     1201 agaccatacg gtcatccctc atcttgctgt caggggcacc cgttataaac tgatctactt
     1261 ctatactgta aacgaatggg agttatatga tctcaaaaca gatccgcagg aacaaaagaa
     1321 cctcataaaa aatccggctt atcagaaaac agtacaacaa atgaaagcag agctgctaaa
     1381 actccgggat caatatgacg atcatgagaa ggccggagaa ctaaattagt catttaccaa
     1441 tgaaaaaagt attggccggg gtcgatcagt tgcttgtgca ggaaaataaa tttcaagact
     1501 atcgtcttgc cctggtaacc aataatgttg ccagcatttc aaccggtgaa tcttcaagaa
     1561 ctgcactgct taaagcaggt ttcaaaatca taaaactatt ttccccggag catggtttat
     1621 cggccaaagg agaagatggg gcattccaaa gaaacagttt tgataccctt acccaattac
     1681 ccgtaatcag cctgtacgga gatcacctga agcctgctcc ggaagagctg tatgatattg
     1741 atatggtatt atttgacata cctgatattg gctgccgctt ctatacttac ctctggacaa
     1801 tgacttatgt gatggaagcc tgtgcggcgt gtaaaaaacc tttaattata ctggacagac
     1861 caaacccgct gggaggtgac cttgaaagag cggaaggtcc tctactcgat gaggaacggt
     1921 gcagatcttt tatcggacga tggaacattc cggttcgcca cagctgtaca atgggtgaat
     1981 tggcaactta ttttgccgca aaacgaataa tgaatgtaga cctcacggtc attaaagttc
     2041 aaaactggaa cagaaaacaa acagttctgg aagcagattg gttatttact cctacatctc
     2101 cggctatctt agatgctgaa actgcattgc tttatgccgg tatgggttta ctggaaggaa
     2161 ttaatgtaaa tgaaggccgt ggtaccggat cacccttcaa ggtttgcgga gccccatgga
     2221 ttaacggaga acaactcaac cgggtattta tggatacccg attacccggg attaagagta
     2281 ctgcaatcac ttatattccc acacatggct tgtatgctgg tgaaaaatgt caggggttga
     2341 gattttttct aaccgaccat catcttttta ctccggtaaa ggccggctta cagttattgc
     2401 aatgtatcat ttcgttatat cctggccagt gtaaagagag attataccct accgttgcaa
     2461 atcctacagg caaagggcac ttagacaagc tcatcggaat atacaggtct tttgaaaaaa
     2521 taggcgaggg agaaattcct tccactatac ttcaacagaa agactggaaa gaaataataa
     2581 gtccttatct tctttattga tactttttca tcagatcaag caatatcttt ttatcacttt
     2641 cattgatgat tttagtagta tcactttgca caaaatggat tttgaaggat tgaatggcat
     2701 tcttttcatt ctttacatca tacccgataa tgcgcaaagc ctggacagga ttaaaatctt
     2761 ttggtacact aatattggtt gtgtcatacc aaagaccaaa ccctttttga gccagtttct
     2821 gccagggaaa attcctgttc gggtcaactt ttctacctgg tgctatatca gcatggccga
     2881 taaaatttgc tgccggtata ttataagctc ctttcaattt tcccaaaact tccatcaggc
     2941 tgttgatctg gggttctgta aaagtctcaa acccgttatt atcaatttca ataccaatac
     3001 tggatgaatt caggtctgta gcattgcccc atttgcttac cccggcatga tgtccgcgaa
     3061 gaaggtcatt gagcatatga tgaactgttc cgtccttaca tattacataa tgtgaactta
     3121 cctgtgtccg ggggagagta aaggtcttta atgtttgctc acaactattt tgtgcggtat
     3181 gatgaatgat gacatagttg ggacgccgca tggaaaaatt agtagtaccc acccattcag
     3241 ctgcagtagt aaaactatcc tttactggat actctgccag cattttggca tattttttaa
     3301 cctgcttttt atagcttttg tttgttgcag catactttgc ctgggagcag ctatatatga
     3361 taatcacaat acctaaaaaa aggcttactc tgaatttata cttcatgaaa agtaattata
     3421 tcggattact aatgtagtga agattttatc ttcttttggg accactctta taaaattttc
     3481 tttttggtcc gctatggcca gcccttgttc tcgggtgata ggaaggagct tcccccaatt
     3541 ccgcaggcac gactgctttc tcaacctcct tgcctaataa ttgctcaata acagcaaatt
     3601 tattctgttc cttttctcca atcagcgtga atgctgcacc atcggcctct gccctggcag
     3661 tacgtccgat acgatgtaca taatcttcgc catcgccggg tacatcataa ttaataacca
     3721 catcaatagt atcaatatca ataccccggc ttaaaatatc agttgctacc agtacgggta
     3781 tgcgacccgc agtgaaagca ctcaacacac tttctctttc ggattgctcc aggtcactat
     3841 gaatttctga aatattaaaa cctccatgct tcagctccct tgtcaattgc ttaaccgttt
     3901 gcttacgact gcagaagatc agtgcatttt tgtatggaat ttccttcaat aattttttta
     3961 tcaatggcag cttttgcggt tcatatacca caaaagcttt ttgtatgatc ttctccggtg
     4021 gtttggatat agcaatatta atttctaccg gttcatgtaa aatctttttg gctaactgcc
     4081 tgatcttttc tggcatggtt gcagaaaata acaggttttg ccgtttcatt ggaagtgctt
     4141 tgatgatttt gttgatatca tcctggaaac ccatgtccag catcctgtcg gcttcatcca
     4201 aaaccaaaaa ctgcaggccc ttcatattta catagcccat attcaaatgg gctatcatac
     4261 gtccgggtgt gcatacaatt atatcagcac ccatctgcag cgcttttttc tcagctacaa
     4321 aattctgagc atcattgcct ccgtaaatag caatcgaact aagatgggta aaataagaca
     4381 gcccttccat gtgttgtgaa atctggatgg ctaattccct ggttggaaca atgatcaatg
     4441 cccccacctg cccatccaca tgattttgca gcaggcggtt catcaccggc aacaaaaaag
     4501 cggctgtttt tccggtacct gtttgtgccg aagcaatcac gtctttccca gccataatcg
     4561 gcggtattac ctgctcctgt acaggtgtgg ccgtttcgta attggatgcc tcaattcctt
     4621 ctaagaggtt aggatgaaaa ttaaattctg taaaacgcaa agtgttcaat ttaagaaaga
     4681 tgcgaaggta actgaatttc aacagatagg ctgattatgt aaaaagacag cgctgattcc
     4741 ctgttgccct ttttgataat aagaatcatc ctttcctatg cgctgcgtca tactacccct
     4801 tttttttgaa ttgtagctta gctattgaaa agaacagtta acacaaaaaa ttagtgctat
     4861 gaaatatagt cttttacttg gagccattat catttctatg gcagcttaca gccaggcatg
     4921 gacaaaacaa tatgatcatg tggatgagtg tacttgcggt ttatctcttg taggcaaaga
     4981 tgataagcat ggatttgttg acaaagatgg caaattggtt gttcccctta tttatgaaga
     5041 cgccttaacc tattctgaag gatttgcggc cgttaaactg aatggcaagt ggggttatct
     5101 agacagcact ggtaaagttt tggtagagcc aaaatatact gaggcaatgt cctttcataa
     5161 cggttttgct gtagtaagca atggtaatga ttatggtttt atcgacacca gtggtaagat
     5221 cggaatccag atgatctacg aaaatgcttc acaattctcc gaaggtttag ctgccgttaa
     5281 taaaaaaggc atgtggggat atattgatac aaaaggcaga gaagttatcg cctgcaaata
     5341 tggctttgcc ggtacttttg gagatggaag tgcaagagtt atgcagaata gcaaatggat
     5401 aataattgac agaaccggta aaaaaatagc tgatagcgaa agataaaata gtttaacgtc
     5461 tgcagaaatg accaaaaagc ctcccgggag gcttttttat ttattattcc atgatctgct
     5521 caaggtgtac gagttgacca tctttggtta tcccttcaat aattacccgg aaagatttgc
     5581 ttacatcatt attataaaaa gtaagtttca ttttattttt agcagctgta gtgactactt
     5641 gcgggttcca gtacaacgta gtacgaagat cttttttttc attggcagca ttaaaactgc
     5701 tgtagttcgg tgaatagaac tgacgtatcg gagtataacc actcactttg ttactggcca
     5761 gtcccttacc gggttccgct tgtccatcat ttccgcggcg ggtataaatt gctattgcac
     5821 cattggtact ggtacctgaa cccgcaaaaa atggtggacg aaaaactttg acataagcga
     5881 tatctgaaac agcaatggaa gaaacaaaat ctgcatccag cggtacttca tccaaaaata
     5941 actgaggggg gcctccccgc cacatcaaag agggtgggtt actgcttgta ttgatctgca
     6001 agccggcaac tttaccttgc aggtattgaa aaatattttg gtaagcaaac ctgaatttgt
     6061 catttaccag gtcaaacttg tagctgtctc cacctgaaaa taaaccactg gaggtatatt
     6121 tctcatccat tatttccacc gatgattttg ttttggtgtt tacaattaca ttctccagca
     6181 ctttcccttc tgattcctgc agtaacctca gcgtctcatc agataaccgg aagtgacggc
     6241 tataaccggt tgtatcaccc agccggttgt agaaaatacc cgttgcaggc atccggtagc
     6301 gaagagccgg taaacggctt tccataaatg ttgccgagcc atcttttatc cctgtagaaa
     6361 gtttgtaata aatctgtgct gtatcaaaaa ggaagtagga gggatcgtta aacgtaccat
     6421 ccggcgctac gggaagcatc accattttat taccatcagc ctggcttttt tgatttacaa
     6481 taagaataat agttgctgca tcccttaatt gtgcgttggt agctccgtac actttgccgg
     6541 aaagagtgag gtaagtgctg tcccggggat acatgatttc aggtaactta ccttttacaa
     6601 catcttccca cttaaaacgc cgccagccat gtgtaagcat taccaggtca agccggtttg
     6661 caattgtatc attattaccc gaaaaataat agcatggatt aaacacctgg cctttaatat
     6721 cgcctgttaa taaaagatgt gaaataatat tttcgctgct gtccgttcca atagccgcat
     6781 cagtaacgga aacagcaaaa ctggcttcca ggctgtcggg caccgtaata agaatttcat
     6841 tcctggccct tttatttaaa ccccaaagtt caactgtcat ttctgcatta aaacgatact
     6901 catcgttatt aatataagta atcctttctg ccaggggatt ccattgccgg tcaaatactg
     6961 taatggtcaa tataccagaa gggaggtctt gtgtaggaat aataccttta acgaaaccgg
     7021 ctgacatatc ttttgtgagt ttaaatacct ggtattgatt aatggtacca acgatgtgca
     7081 aggtaccgag atctttagcc aggttctgct cagctgtaac agcaaaggtt cgcttaacac
     7141 ctgataaagt tacctgcaag gaaacacctg ttggcttaat agccggcaat gctgtaacat
     7201 actcagttcc attttcatct ttccattttg ctgtatagct ttcacccagg cggggaaaaa
     7261 gaaaaaaata acccatcccg tcatgtatga tgcggaggct gtctatctcc tcccctatac
     7321 tgctttgtac aagtccttta attttaaccg gcttacccca ttgatcagtg accttaaaag
     7381 caattttgtt tgaaaggcct gctacagcat ctccgccctc cggaaaaaat tggagggttg
     7441 gtacaaccgg aattttagca gccggagtat ttccattttt tgtaatgatg cgaatgtctt
     7501 tttcatataa aaaagcggaa tcaaaattca acatccattt ggtgtaagcc cttacatgaa
     7561 tgaatgtacc ggtataatca gcaggaatat caaactggcc attgctggta gcatccacaa
     7621 ccgggcttac gccgtgcaac agaagattac cagcttcatc agtccagtcg gtataaaatg
     7681 ttttgctgtc atcggccggt gaaatggcct tcatcaggta ggccttgaac caaatcgttt
     7741 cacccggcac atacgccgtt ttatcgtatt gaagataagt acgttcctgg ccgtattcat
     7801 tggcataccg ggcaatggtt gcatcaatat tttgagcctg aagaccaaca cagattaaga
     7861 gaaaaacaaa aagaatatca gccctttttt ccatcataca gctaattatt tcctgaacac
     7921 cagtaattgc aaattagtgg cataatgaaa atacaaaatg tcccttaaca gtttagcaac
     7981 acctgaaaca gaaattacat atttctcctg tattgcccac cgacttcata caaggcgtga
     8041 gtgatttgtc cgagtgaaca atatttcacc gtttccatta attcctcaaa aagatttcca
     8101 ttgttgatgg caacagcttt taaacgctgc agcatgtcag ccgatttatt ttcgtttcgt
     8161 ttccaaaaac tccgcaacgt ctgtatttgc tgttcttttt cttccgttgt gctgcggatt
     8221 acttctgtag gtaaaatagt cggacttcct tttttgttta aaaaagtatt gacgccgatc
     8281 agtggcagtt ctccggaatg tttctggtgc tcgtaaaaca aactttcttc ctgaatctta
     8341 ttccgttgat acattcgttc cattgcgcct aacactccgc ccctttctgt gatacggtca
     8401 aactcagcta ataccgcctc ctccaccagg tcagtcaatt cttctatagc aaatgaaccc
     8461 tgaataaaat tttctgtttt tgcagtaccc agctcccggt taatgattaa ttgaatagcc
     8521 atcgctctgc gaacactttc ctccgtaggt gtggttatcg cctcgtcata tgcatttgta
     8581 tgcagagaat tgcagttatc atagatggca tacaaagcct gcaatgttgt tctgatatca
     8641 ttaaaatcta tttcctgtgc atgcaaactt cttcctgatg tctgaatgtg atacttcatc
     8701 atctggctgc gtttattggc cttgtattta tacttcatgg cttttgccca gattcgtctt
     8761 gatacacgac caatcacact atattcagga tccattccat tactgaagaa gaaagaaaga
     8821 ttgggagcaa aatcatcgat attcattccc cggctcaggt aatattccac gtaggtaaac
     8881 ccgttggcca gtgtaaatgc taactgcgtg atagggttag ctcctgcttc tgcaatatga
     8941 taaccactta tggagaccga ataaaaattt cttactttat tattaataaa atactcctgc
     9001 acatcaccca tcagtttcaa tgcaaactcg gtggagaaga tacaggtatt ctgcgcctgg
     9061 tcttctttta aaatatccgc ctgcaccgtt ccccttacct gttgtaatgc cgtttgctta
     9121 cagtggtcat aaatttcctt tggcaaaact tcctcaccgc tcaaacccag caatttcaac
     9181 cctaatccat cattgccttc aggaagctct ccgggagacg atgaattatg gtattttggc
     9241 ggaggaaaat gtttgaattt atctgcactt atctttctta ctttatccca caggcccagc
     9301 tcagttattt tcttttcgca ctgctggtcg atagcggtat tcataaagaa agccaacagc
     9361 ataggagctg gcccattgat ggtcatagag actgaagttc ttgggtcaca gagatcaaat
     9421 ccactataaa gcttttttgc atcatcaaca gtagctatgc taacaccact attgcccact
     9481 ttaccatata tatcaggacg atgatcagga tcctccccgt ataatgttac actgtcaaat
     9541 gcagtagata acctttttgc cggttgatca acacttacat agtgaaaacg tttattcgtt
     9601 ctttcaggtc ccccttcacc ggcaaacatt cttgtcgggt cttccccttc tcttttcaat
     9661 gggaatacac cggcagtaaa aggaaaactg cccggtacat tttcttgtgc ctgccaggta
     9721 agcaggtctc cccagtcttt atattttggc aaaaccactt tgggtattct tgtaccggat
     9781 aagcttttac tgaacatttc ctgtttgatc accttatccc tcactttgta ctcataaaaa
     9841 tctttttggt actgagctgt tttattcggc cagtttgtaa tcagtttccg ggcaaccggt
     9901 gttagctgtt cagtataaaa attgatttgc ttttgtataa tttccagcaa agacttatcc
     9961 gcactctctt ttaatgtctc cagcgaacca tttagctggt ataattttga tgcaatagct
    10021 gattgttctt gtaccaactg gtcgtagcta cggttatttt cagcaatttc agaaaggtat
    10081 cttacacgat tgggtggtat tacggcctgc gacactttag ctgtttctgc aaaatgatac
    10141 ttgccaaatg aagtgttggt tttagcatca atctttttca gcagtagttc aaacaaatga
    10201 ttcactccgt catcgttgaa tttactggcc atagtaccaa ttattggcag ttcttcatct
    10261 atggctgtaa acaactggtg attccgttta tattgctttc ttacatctgc cagtgcatcc
    10321 aatgcccctg ccttatcaaa cttattgata cataccaggt cagcatagtc cagcatatta
    10381 atcttctcca gttgtgatgc agcaccatat tccggcgtca tcacatacat acttacatca
    10441 caataatcca ggatacttgc atcgctttgt cccacaccgg cactttccaa aataataaaa
    10501 tcaaacccgg ctgctttaca gatatcaatg gcatcctgca tggcactact caaggcagtg
    10561 ttatcatccc gggtggccag gcttctcatg tatgctcttg gatgattaat tgcattcata
    10621 cgtatcctgt ctcccagtaa tgcaccaccc gtttttttct tggatgggtc aacagaaata
    10681 actgcgattg ttttttcttt gaaattattc aggaagcgac ggacaatttc atccgtaaca
    10741 gaagatttac cagcaccccc ggttccggtt attcccaaca caggaattct gccaactcct
    10801 aactccttgc tcccggcatc taacaccact ccattttctg catttgtaat cttccttgca
    10861 atcaccctta tatcttttgc ttcttccagt ttcaaggccg ttttataatc cgcatttcca
    10921 ttcaaatgta tgtcgcactg ctttatcaca tcttcaatca ttccttctaa ccccatatgt
    10981 ctgccatcat caggcgaata gatcctggcg attccatatt tatgcaattc atctatctca
    11041 tcaggcagaa tagtgccacc accaccacca aatattttta tgtgcccgca gccattttca
    11101 tccagcaggt ctttcatgta tttaaaaaat tcaacatgac cgccctggta actggtaatg
    11161 gcaatcccct gcacatcttc ctcgattgct gtttctacaa tctctttaac tgaccggtta
    11221 tggccaagat ggataatctc ggcacctttg ctctgcataa tgcggcgcat aatgttaatg
    11281 gctgcatcat gaccatcaaa caggctggcc gccgtaacaa tacgaacctt attggaagga
    11341 gtataactca taaattataa gaaaaagcaa atttaactta aaaaactaat gcctgttagg
    11401 gcattcaatt tacaggattg cagtctgtta actacatttg tctcatgcag cacctgattg
    11461 aatctatcac ttccctgtac aaaaaatgga aaggcgcaga ccctgtaact gttgacctgc
    11521 tgccacaaag tggcagtgaa cgacgttatt tccgtcttca cagcaaagat gagacagtaa
    11581 taggcacata tggtgctaat ataaaagaga atgagacatt caattatttc tccaaacatt
    11641 tcaaagaaaa aaatcttgct gtcccggaaa tactggccat cagtgacgat ggtatgtttt
    11701 acctgcagga agattttggt gacatttcac tgctaaacca cctggaagca aaaggattca
    11761 cagaggaagt gtatgagctt tttaaaaaaa gcctggaagc cctggcacaa ctacaggtga
    11821 aaggtgataa aggattagat tacaataact gcctgaccaa tacagaattt ggcaaacagg
    11881 ctattatggc tgatttattg tatttcaaat actatttcct ggatgcattg cgtaagccat
    11941 acgacaaaca gaaactgatt gatgattttg aagcgttgag taattacctc acccatacag
    12001 aatacaaata ttttatgttc cgtgattttc aaagccggaa tattatggta atggaggaca
    12061 atagtgtaca ctttatagat taccagggag gtatgaaagg tgcccctcag tatgatgtag
    12121 cttccatgct ctggcaggca agagctaacc ttccggataa atggaaatat gatctgctgg
    12181 aagattatat tgacgccttt gaaaatacaa tcggaaaaag tgtaaacagg aatatttttc
    12241 agagccagta caatggatat gtcctgatac ggctgctcca ggtattggga gcttatggtt
    12301 tccgcggatt atttgaacgc aaagctcagt tccttaccag cattccgtta gccttaagaa
    12361 acctgaggga ttttttccag caccagagta ttggcatttc agtgcccgaa ttcagaaaag
    12421 tactggatct ctgtgtggcg gatgagatta tccagcaatt cacgcctaca caagcaactg
    12481 aaaacactcc tttgcttatc aagatccaca gtttttcatt ccggaaagaa ctgccccggg
    12541 acaatagcgg gaacgggggc ggttttatat ttgattgccg cggcatttta aacccgggtc
    12601 gtatcgagag tatgaaaaca caaaccggac gggataaaga agtcaaagat tttttggagc
    12661 aacaaaccaa gatgccggaa tttctgaaca gtatatttga catcgttgac attactgtag
    12721 aagaatatat caaaagagat tttgaaagcc tgatgataag ttttggctgt acaggcggac
    12781 aacacaggag tgtatatgct gctgatgcaa tggcaaggca tctcaaaaat aaatttaaag
    12841 tcaggattga actcctccat attgaacagg aagctaaaaa ctggataaac cccctcccgg
    12901 agaaaaagta atgaaagcaa tgattttttc agccgggctc ggcacaagat ttaaaccatg
    12961 gacggataaa catccgaagg cgctggctgt gatcaatggc aagtcgcttt tacaacggaa
    13021 tattgaatat ttgcagcaaa atggcatcag ggacgtcatc gtgaatgtgc accattttgc
    13081 agagcaaata gaagaagcta ttgtgtcaaa cgatggatgg ggaagtaatg tattggttag
    13141 tgacgaaaga gacgaagtgc tggaaacggg tggtggttta ctgaaagcag aagaattgtt
    13201 tactacaggt gaaaagttta ttacctgcaa tgcagacttc ttaacggatt taaatatcca
    13261 aaaccttatt cattaccaca atgagaagaa agccctcata acttttggtg ttaccaaccg
    13321 taaatcatcg agggtattgc tatttgatga tcatatgcgg ttaaccgggt ggcggaatac
    13381 tgtaacgggt gaagagcgaa tcgctattcc ccctcctgct gagggggtta gggggaggcc
    13441 actggcgtat agttgcgttg tagtttttga ataccgcatt tttgaactca tgaggcaata
    13501 tggttttatt ggaaaatttt cactcattga tgtgtacctg aaactggccc ccgaacatct
    13561 tataatgggc tatgaacatt ccggtgatcg tttggttgat gtgggtaaac cagaaagtgt
    13621 tgagatagct gagtcacttt ttccataaag tattgcaaat accctctaag cggtagcctc
    13681 cttgcgtcct tttattctat ctttcgtttt taaatatacc atatgaagaa gttcgtctcg
    13741 ttcatctgca gccttttcct gcttgttagt ttatcagcac aatcaaaata cgatcacaaa
    13801 gaagctttta atccgcaatt ctatccctac cccggcaatg attacagaag tgccagtggt
    13861 gaacccggcc caaaatactg gcagaataaa gcggattatt ctatcaattg taaattagat
    13921 actgctacac atacagtcac cggagatgta gcaatcactt ataccaataa cagcccggat
    13981 aaccttcgct ttttatggct gcaaatggat caaaatattt ataaaaaaga ttccagggga
    14041 tcagccacta ctacacaaac aggtggacgc tgggccaatg ccggttttac agatggatat
    14101 actattacca gtgtagaact ggaaagtgca ggtgttactt atgcacccaa taaaacaata
    14161 acgaccgata cccggatgca aattattctt aaagaccaat tagaaaaagg agaaaaagta
    14221 aaaataaaga tcagttacaa tttcaccgtt cctgaatatg gaaccgacag aatgggtcgt
    14281 ttaaaaacaa aaaatggcat catttacgaa gtagcccagt ggtttccccg aatgtgtgta
    14341 tatgatgaca ttcagggatg gaacaccttg ccctatgttg gtgcaggtga attttatctt
    14401 gagtatggca atgtcgattt cagtatcaca gcaccttccg atttaattat tgttggctcc
    14461 ggagaattga tcaatccgca ggattgttat acagccgaac aaatgaaaag atgggaaacc
    14521 gccgccaaaa gcgataaaac agttaccatc cgcagcgata aagaaataac ggacaaaaat
    14581 tcaagaccca aaacagcaaa cacaacctgg cgattcagca ttcaaaatac aagggatgtt
    14641 gcctggtctg cttcaaaagc ttttgtacag gacgctgcaa gaatcaatct caagagtggt
    14701 aaaaaatgta tggccatcag tgtgtacccg gctgaaagta tagtaaaaaa tggctggcag
    14761 cgatctactg aatttactaa agccagtatt gagcattatt ctggtaaatg gtttgaatac
    14821 ccttaccctg ctgctaccaa tgtagccgga atagtaggcg gcatggaata tccgggtatt
    14881 gtattctgta gttccgaagc atccggcaca ggactttggg gagtaactga tcatgaattc
    14941 ggtcatacct ggttccccat gatcgttggc agcaatgaaa gaaaatatgc ctggatggat
    15001 gaaggcttta atacttttat caatgatggc tctacggaat catttaataa cggcgaattt
    15061 aaagaaaaca gcttttttgg cgacccaaac tcatctttca tggtcaagta cacatttggt
    15121 gataaaatgg acggattgtt caatacacct gatgttatac aacaggataa cctgggcatc
    15181 gctgcgtata tgaaaccggc acaaatgctg actgcgttgc gtgatgtggt attaggaaaa
    15241 gaacgctttg atgcagcctt tgccgaatat atccgccgct gggcatttaa acaccctacc
    15301 ccctgggatt tctttcatac aatggaaaat gtttcgggag aagaccttag ctggttttgg
    15361 agagcatggg tattaaatac atggaagctc gatcaaacag taaaagcagt ggcttatgta
    15421 gatgaaaaac ctgagaaggg tgttgaaata accatagaga atcttgaaaa aatggtaatg
    15481 ccggtagcag tattggtaaa agaatccaac ggtaaagagc acaaaataaa tttacccgtg
    15541 gagatctggc agcgtggcgc cgaatggaga ttcaatgtgc ccaccaccag tgaaataaaa
    15601 gaagtgatac tggaccctga caagaaacta ccggaatggg accgcaccaa caatagctgg
    15661 aagaaaaaag gattttgatt aaaagacctt ctgcagttct gcagaaggtc ttttttaata
    15721 tagcttataa tgaaaaaaat gattctgctt attatcatta gtagtacaat atttcgtgta
    15781 accgcacaag tgaatgaaat gaaattctct gaactattct tattaaaagg cacctggagt
    15841 atgaaaacaa caaaagggat gctttacgaa aactgggtga taaataatga tagcacaatg
    15901 actggcaaaa gttacaggct gaacaataca gatactattc ttttagaaac agttggtctt
    15961 gttatgagag ggagccagat cctttatata gcctctgcag aaggtcaaaa taaccaacag
    16021 gcagtagcat tcaaacttgt gaaccgggat aacgatactt ttatttttga aaatccggag
    16081 catgattttc cacaacgggt tatttatgaa ttaccaaaac atgaaaaatt acatgcctgg
    16141 atagaaggaa cgataaatgg tcagtccaga aggtccgact atcattataa aaaaattatt
    16201 taatgcactc aaagctgccc catcagctgt tcaagcgaaa cttcttcgag taatactctt
    16261 tctgtcaaag catttttccc tgaccacttt accaggcgga taaatccatc tgcgatctca
    16321 atacctgtta tatcaccatc atcaaagcaa caacaacccg aattgaaata agaaggcttc
    16381 attttcatat aatcaaccga tacggcagta aaatcatttt cccgtctcct gatctctctc
    16441 cgtatttctt cttcccggtt tttatcattt gtatgaatgg cctgctgaaa atttttatat
    16501 aggcgttcaa ggtatgtcag ggaaccaaat acaggttgat gtgtatgacc cgttatcagt
    16561 actacatttt tttgctctga tgaccattcg tacatgatgt tattatgcag cgtcttcttt
    16621 tccgcatcgt aagccggagt attaggatta atacggagaa aagcctgcag cggagcccag
    16681 atattggcta caaagaattt gctgaaccag ttaccatcac tttgtgcatc gccctggtga
    16741 ccatgagtga ggaaaatagt aagtggcgag ccactaatgg gtagttcagc gtctgctgta
    16801 ttcattcccg gctcctggcc cctggccagc aactctaaaa tcactccttc atacactttg
    16861 atcttctctc cataaatatc tttcaaatgc caccaggcca gtggatcatt gtcccaaaac
    16921 aagtcatgat tgccaaatat ttttatgtat ttactccgcc tgatgaattt tttctccgta
    16981 tcaaatactt ccttgttctt tttctttact tgccagatat tgttttccca tagctcttca
    17041 ctatctccca gattgataaa acaaaaatca tggccattat aatactctaa agcgtggaga
    17101 taatttgctt cgcacggtgt aaaatcatca gcgccgtttc ttcttccttt atgctggtca
    17161 gaaaagatta taaacttccc ggtgctttcc acaaaaggta ttaccagacc ttttttaccc
    17221 ggattagtgt gtatttcagt atataattta cccagtgctt caaatattct ttcccttttc
    17281 ggtttggaag agaatttagc agcaattttg agaatcggtt tatataatat gatacgaaga
    17341 aaatttctca tcagttgtac tattacaatt tataccaatt ctctttaaaa tatatatggg
    17401 ggtgaaaatg ccacggagaa tctatcgaag ccttttgatt ctctcttcaa taatcgtatg
    17461 gagacatact ggtactttat ctctccattg cagtaaagaa aaacccgcca ggctataaaa
    17521 ctggcgggtt aattattact cattctctta aatcttatct tacatgcact cttccttctg
    17581 ccagcttcat tccatttaca tctccaacca ccacataata aattcccctg ttagcttttt
    17641 cgagatttac aggtatcaat gtataagaac cagcgacagg gaacttacgg tcaaatacag
    17701 ttgatccttt tgaatcaaaa attatgattc gcctttgtgt gctgtttcca ccattgttat
    17761 aatacgaaac ggtaaagttg ccatcattag gacttgggaa tatgaacagt ttgctgctaa
    17821 cagatgcatc gatagtcacc acctgtgaaa ggctggagca gaaaatactg ctgggccagg
    17881 tttcctgtat tcttacctgg taagagccag gattagcaac agttacaggc aaggtgttgc
    17941 cacttaccgc aatggggtta ttatttaata gccatgttgt aacaagtgtt cctcctgttg
    18001 aggaagttgg tgtagccgtc aaggttgtag tttgtcccgg taataaactt gtcagcggcg
    18061 ctgcggttaa tcctactgcc ggcagttgac gaacagtcaa tattgcagca ttggaagcca
    18121 caggagttgc acaagtgaca tttgacaata aacaacggta cctgttgtta ttcataactg
    18181 caataacgcc tgtagccgta aaagttgcag cattagcccc ggcaatattc gtccagctgg
    18241 taccaccatc agtgcttacc tgccactgat aaatgattga cggaacactg ttcccagaaa
    18301 cgataaaact tgcatcactt cctgaacata cttcagcatt tgccggtgaa gaagtaacaa
    18361 cgaccggagc atgaacagtt aatgttgcgg cagcagaatt agctgcaggg gaacaagtac
    18421 ccgtaaccac acaacgatac tggtagccat ttaatccaat agtagctccg gttatagtta
    18481 gtatacttgt tgttgctccg gcgtactctc cgccattatt aatatttgca aagccacttc
    18541 cggtatttac ctgccattga taattgatac ccgtaccggt agcagttaca ctaaatgatg
    18601 tattaccacc aacacagatt gtctggttta caggactacc tgttatagcc ggtaatgcat
    18661 ttacagttaa tgttgcagca ttggatacac ccggagttgt acaggagggg tttgataatt
    18721 gacatcggta ctgatagcca ctcatagctg ttgtagcgct tgtaatgacc agcgtagcag
    18781 cagtagcacc agtgtaaaca cctccattgc tgatatcagt aaatccgctt cctgtgttaa
    18841 cctgccattg ataaataata cctgtaccgc ttcctgctac agtaaagctt gtgctggcac
    18901 cgtcgcaaat tgtttggtta acaggttgtg tggtcacact aacggcagta gctacagtca
    18961 gtaaggcgca gccggatgtt atggttgtcg gaccacaggt acctgatact ttacacctgt
    19021 accccgtgtt gttcatacca gcagtaacag atggtatggt ataacaggaa gaagtagctc
    19081 ctgcaatatt tgtccaggga ccgccgcaac cggccggact tatttcccac tggtacgtaa
    19141 ggcctgcacc ggtagcagta acacaaaacg tagcaccact gcctgcacat aatgtaagat
    19201 ctgttggttg ggcagttatt gaaacaatag agtttactgt taatacagca gcatttgaat
    19261 tagtgctacc gcattgatta cttaccacac agcgatactg gttattattt aatgctgtcg
    19321 ttacaccagt gatagtataa gaagaagttg tttgaccgga tacattcgac caggtgccgc
    19381 caccattagt gctcacctgc cattggtaag ttccggttgc cactacacta aaagttacgt
    19441 tggcaccttc acaaactgtt tggcttaaag gttgggtagt gattgctggc ccgcttccgg
    19501 ccgttattgt atatgtaaga tctcttgttt ggttggtagc gcctgttgca gtacctgtca
    19561 ctgtgattac ataggaacct gaggccagtg tatttgtacc gtttaatgta atagttgatg
    19621 aactacccgg gttaacagga tttggggaaa aactaactgt tgtaccaaca ggattacctg
    19681 tagctgacaa tgtaatagga tttgaaaacc ctccagcaga aattgttcct aacgaaatag
    19741 ccatacttgc aggcgcagga caggctgcag aagcaggtgc tggagaagtg aacgaaaatc
    19801 cggaaggtaa agcagatgaa actgttgccg tccagatacc tctgccatga gtacctgcta
    19861 caatcgtacg atcggaagaa cgatatttaa tcatatctgt tctgacatta ggaaaagtag
    19921 cattggcatt ccatactgta cctgaaccat taataagatc cgtttcccat acaccggttt
    19981 cagttgcaat aaatgctttt gcattatcat cggggtgaaa taatgcccag cgtaccggca
    20041 tattgggaag gtttccgtca ataccagtcc aggaagttcc gccatttgtt gacacccata
    20101 cattcattac attataatta gaataacaag ctatcaggtt ctgatcagag gaccccgtca
    20161 ccacacagtt tacatagcct gttgcaccag cgggtgttat attagcttca attggagtag
    20221 caccattagc atcatcaacc cgcactactc ttccattacc tgttccaaag taaaccctgt
    20281 tagcagtata aggtgaagca tgaactgcag atacgttttg tcctgcaaat tccccaattg
    20341 agattatact ggtagtactg cctgtttgcg gatcattcca tcgaaaataa gtaccccctg
    20401 aattacaagc atagataata ttagcattgt tgtcatagtc ccatggatta ataaaccgcc
    20461 cgcttgaagt aatattaacc ggtgtactcc agttggcgcc accattagta cttcttctgt
    20521 acacattatt gacataagaa ccgaactgga actgaggttc attttgatcg atagccacga
    20581 aaccaccgtc accgcccgtt acttccactg atgctccaag tccggcgttg gccaactgat
    20641 gtacaccatt atcctgtgta cctccgataa aatagttggt agtggtgggg tgtatagcca
    20701 ctgaataaaa ttgtttcaat ctcaaaccca cgttcctgtc acgtattgta atgcccttgt
    20761 cagcagaaaa atgcatacca ccatcacttg caaacaacaa cttatttcct ccatcccacc
    20821 actgaacatc atgctgatca gcatgtacat attgaccgga ggtacccacc caaccagaaa
    20881 ttcttgccca ggtatcacca ccattaatag ttttagcaca atcaatgccg ccaatgatac
    20941 attcatcagg atttgcagga tttatgcgaa cagaaagatc ataccaaccc tgggtattgg
    21001 taaatgcagc cccggtaacc gtagttggag gtatccctgc tgttgcaacc cagtttacac
    21061 ccccatctgt tgatttgtag acattggtaa cctgcccgga attattacag ggcaaggaat
    21121 aaagtatatc accgctgact gcaatttctg ccctgttatt aaatgaagga aaagctgtaa
    21181 ccggcgcatt ccaaccagca ccagtagtta ctgtagccgg aacatcagta tagcgataga
    21241 attgtgctga attaattcct gtaaccatat gaagccttgc ggcagtggcc gtagaactta
    21301 tttccaggtc acaaatcctg ttacccacac cagaaggtgt aatatctgtc catgacgatc
    21361 cgccgtcaac agatctcagc aacccgttac cacgggtagc caagtaaaca tttccctgga
    21421 agtcacaaag aattctggta caggcggtat aagcactggt gctggaaaga aaattccagg
    21481 aagctccacc atctgtactt ttaaaaacac ctacaccttg taccgcgtca aaattaaaat
    21541 atgactcacc cgtacagaaa tacattgtgt tttgaaaacc gggccttgga tcctggcaaa
    21601 tggctgcaat agcgaggtta cttaaaaaat ctgttactgg aatccagtta gccggggatg
    21661 ttgttatatc agtggttttc cataaacctc catctacacc acccacaaat acagtttttt
    21721 ttgttggatc taatgaatcg atcatcacag ctcttattct tcctgatgtc actcctccgt
    21781 tagccctgga atttccattc gatgggccta caacatcact atccggacct ctttccgtcc
    21841 aggataatgc ccctatggca ttatttctcg aaaactcttt ggcctgttta gtttgttcaa
    21901 tggccaataa cagtttattc cagggaactt ttcctgtagc aggatccttg gtacgttcta
    21961 tctcccaagc agctctttgc tccgggccgc tgtattcacc accctcttcc tggtcggata
    22021 ctttgttttt tgtaccacag ccaacagaca ataaaagact gcctgttgcc agtaaaaggc
    22081 tcagcaatgg taaagaataa aatttcttca taatgggaga attgcttttt ttgttttttt
    22141 gaaaataaac gacacaaggt aggtaaacct gctaaatatt gctgtagaag aaaatcggat
    22201 tactgatgat ctcagaaagt tatttacctt aatcaactat ttacggcaat catacttatt
    22261 tctacgttca caaacttagg aagggccgat acctgaaccg tttccctggc cggaaaatcg
    22321 cccttgaaat acttaccata ggcttcatta ataacgccaa attgttgcat atcggtgatg
    22381 aaaatggttg tcttcactac atgaccgaaa tccatacccg cctcctgcaa aatggctttc
    22441 aggttatgca tcacctggtg agtttcttcc tgtatatctt tatttttcaa atcaccggtg
    22501 gtggggtcga tgcaaatctg accggaaata aaaagcatat tgccggttaa taccgcctgg
    22561 ttgtacggac cgattggcgc tggagcactg gttgttttaa tgatagattt acccataagt
    22621 aataattata aaattcaata gaacgaattt agctttatac gttttctttg caaaaaatag
    22681 tattatgact atcgcagttg tggccagcga agctttcaag gaagaattgc tggcgcaagg
    22741 cttaaaaagt gatataaagg cagaatggtt aaacaaacct gcaaatgttc ccggaacagc
    22801 atgttatatt gaccttttgt tcagttcagg ccattctgag gaattgctta agctccaacc
    22861 ggcattgatc attgtcaatt ccgttcctga tacaacagcc agccttccgg aaaattttat
    22921 ccggatcaat ggatggaaca cttttttaaa aagacctatc gtagaagcag cttgttctga
    22981 tattccggcc agaataaaag cggaagaaat ttttgcagcg ttcaacaaaa caatagaatg
    23041 ggtaccagat ataccaggtt ttattacacc cagagtaatt gccactgtta tcaatgaagc
    23101 ttattttaca ctggaagaaa aagtaagcag taaagaagaa attgatattg cgatgaaact
    23161 gggcaccaac tatccatacg gccccttcga atggagtaaa aaaataggac taaaaaaaat
    23221 atttgatcta ttaaccttac tggcaaaaac aaatgcccgg tacgaacctt ctcccttact
    23281 aaaaaaagaa gcactcttat aaatggctct tattttaaat attgacactt cgcttgatac
    23341 agcatcggtt tgccttggaa acaatgatgg agttttacag gtggcaatta acgataatca
    23401 aaaagaccat gctgcctgga ttcatactgc aatcaataaa ttgctgaaag aaacaggctt
    23461 tccgttaaaa agtcttgaag ccattgcagt tagtataggc cccggttctt ataccgggct
    23521 cagaataggt ctgtcttcag ccaaaggctt gtgctatgcc cttgatatcc cactaataac
    23581 aatcagtaca ttaaaaatga tcgcatctgc cgttaaacaa aaagcaaccg atcttatttg
    23641 cccgatggta gatgcaagaa gattggaagt gtttactggc ttgtacaata aagacctgct
    23701 tgaaatttcg gcagcccggg ctttaattat tgatgaaacc agtttcgata catttctttc
    23761 cagccataaa attctttttt gcggtaatgg cagcaaaaag ctgcaaaata ttctttcctc
    23821 tccaaatgcc agcttcattg aaactattcc aaatgcttca gagctttccg atttgtcaca
    23881 gaattgttat ataaacaaac aatttgcaaa tcttgcctat tcagagcctt tatacataaa
    23941 ggaattttat actcccggcc gaaaggatta actgaaaaat tcgtcttatt aacaattatt
    24001 tggacaaacc cggtttgtac aagggtattt ttgttccttc ttttttcatt aacctataca
    24061 gattctgaat tatgagtaat tattcaatga cacttactga cccgaaagaa aacggggttc
    24121 tgctgaaagt cgacttactg gaagtaaaaa aagcagcatt ggtcctcaga gcgcttaatc
    24181 ataagctacg tcagcaaata ttgaagctga ttgatgaaag tggcaaaatg actgtgactg
    24241 agctatatgt gaaactcagg ttggaacaat cagttgcatc ccagcatttg gctattttac
    24301 gcaaagccgg atttgtaaaa acagaaagag atggcaagtt catttattat tctgtgaata
    24361 tcaaccgcat acatgaatta aaccgattcg ttggtgaatt actcaactga tttataattt
    24421 atttagataa gcagccccgt ttacttaaca ctaaacgggg ctttttttaa taggtaataa
    24481 attatttttg cccggttact gaaaacaaac aaaaaccaga catatgttta tcaaacaatt
    24541 atatactggc tgcctgagtg aggctgccta ttatattgaa agtaatggcg aagctgctgt
    24601 tattgatccc ctccgtgata tagatgctta tcttgaattg gctactgaaa gaaaagccag
    24661 cattaaatat attttcgaaa cacattttca tgcagatttt gtgagtggtc acctggacct
    24721 tggaaaagca accggtgcac caattgttta tggacccggc accctcacaa aatttcctgt
    24781 tcatgttgcc agggatggtg aaaccttcac aataggaaaa cttgccttag aagtactgca
    24841 tactcccgga cacacactgg aatcaacttg ttttttactc aaagatgaaa acgggaagga
    24901 tcattgcgtt tttaccggtg atactttgtt tgttggtgat gtgggccgac ctgatcttgc
    24961 acaaaaaggt acagaaataa caatggctga tctcgctggt atgatgtatg ata
//
LOCUS       DAORTI010000041        24971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1551_length_24971_cov_8.123375, whole genome shotgun sequence.
ACCESSION   DAORTI010000041 DAORTI010000000
VERSION     DAORTI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24971
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_24971_cov_8.123375"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLO70_07300"
     CDS             complement(<1..795)
                     /locus_tag="PLO70_07300"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ74306.1"
                     /translation="MSFFLFIFARMNKKRIIKWAKIVAIIYISGGVALYFLQDAILFH
                     PVTLNRTYSYNFPEPHKDINIPIDSKDTLNLVDFASTDTIARGIVLYFHGNKRNISWY
                     AKYIPYFTRHGYQVLMIDYPGFGKSTGKLTEQKLYDWALQVYKIARKRFPADSIIIYG
                     KSMGTGIAAQLASTRDCKRLILETPYYDFPAVVSHYLPIYPAKWMLHYQLPTYQYLQN
                     VTAPITIFHGTKDRVVTYKNSKRLLPFLKSTDELISIEGGSHNNLYR"
     gene            complement(827..1975)
                     /locus_tag="PLO70_07305"
     CDS             complement(827..1975)
                     /locus_tag="PLO70_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74307.1"
                     /translation="MKLKLYILLLTGISISAVSCKTASKLYEKGNYTEAVELAAKKLQ
                     KDPDDPKLLNIIQSSYRYAVNDHETRIRANAESSNELKWEWIYNEYVSLQRMYDAIYR
                     VPQVFTIVNPADYSSYLVTYREKAGDTRYDRGIAFMQRYDKQSYRNAYREFQAALKFK
                     PGNRDVIQKMNEAYDYAVTNVVILPMQQQGGYVYSSYTVGGNNLDDQLIRNLQYNSGN
                     EFVKFYSAWDARSQHIRVDKEVDMRLTNLDIGRYYDNRSTRKVSKEIVVKETVYKPDS
                     IVKEYARVFANITTIKRTLQSTALLQVNVRDENGRRLWSENFSSNHNWSTEFSSFTGD
                     ERALSENDKQLINRRREFAPTESEIMRCLLDEISNNALYRIKSYFNQY"
     gene            complement(2073..2861)
                     /locus_tag="PLO70_07310"
     CDS             complement(2073..2861)
                     /locus_tag="PLO70_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ74308.1"
                     /translation="MPVFIWYIIIGIAALSVIAYFIQERLIFKPEKLKQDFQFKYDAP
                     FREYFFDIEPGVRINGLHFFRENPKGLILYFHGNTRSIKGWARYAKDFYRYDYDVLLL
                     DYRGFGKSTGKRGEKQMLNDMQFIYNKLREKYAEDHLIVYGRSMGSGFATKLASDNSP
                     RYLILDAPYFNFLRVIERFLPILPVRIVLRFHLRTDKWLPNVKCHTYIIHGTKDWLIP
                     IRHSEALQKINQHKITLIRIHGGGHNNLPSFDEYHNFIRDILKY"
     gene            complement(2918..4894)
                     /locus_tag="PLO70_07315"
     CDS             complement(2918..4894)
                     /locus_tag="PLO70_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQZ74309.1"
                     /translation="MKVSTNKVTGAGLLIALGIIYGDIGTSPLYVFNAIIGNRVVTEE
                     LIVGTLSCIIWTLTLQTTIKYVILVLRADNRGEGGTFALYALVRRRKKWLVIPAMVGG
                     ASLLADGIITPPISITSAIEGLKELPSLGIEDGSNTVVIIVLSILALFFFMQQFGTAS
                     IGKLFGPIMFIWFTMLLILGIIHVFDDLAIFKALSPYYAFEFLKNYPEGFWLLGAVFL
                     CTTGAEALYSDLGHCGRGNIRSSWIYVKTCLLIHYFGQGAYLLAHRRGLEITSEVKSS
                     LSINAFYDLMPHWFIIPGVIIATTAAIIASQAMVSGAYTLISEAMRLNLWPKLRIRYP
                     SEAKGQLFIPTINFLMFLGCAGIVLYFRKSSNMEAAYGLAIIVTMLATTILFANYLVL
                     HRAKSIWIYIFLGGYLIVEIGYLVALMVKFTHGGYITLIIGMLVFLVMYIWYRARKIK
                     NRYVEFVRLEHYIPKIQELSNDKSVPKYATHLVYMTSANNPKEIEHKIIYSILNRKPK
                     RADIYWFVHIDTVDDPYTSEYKVEHIIPNDIIRVDFRLGFRMEPRVNLMFRKVVEDLV
                     ANKEVNIISRYESLASSNTVGDFQFMVMEKYLSQDNELPFFERVIMKFHFWLKDHSLS
                     EEKGFGLDLANVTVEKFPLIVAPVTNLKLKRVEE"
     gene            5198..6733
                     /locus_tag="PLO70_07320"
     CDS             5198..6733
                     /locus_tag="PLO70_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74310.1"
                     /translation="MCTMFGKKWIISVLAMSMIMPATAQKIIYSDPDKDDTRRLNFEI
                     VGKINGNFLIYKYVRNKSWITVLDNDMKQIAREEQDYVPANDRVINVDFFPYSDHAYM
                     VYQYQKRNVVYCMASRIDGNGKKTGDVIALDTTHIGFAANNKIYSVLSSEDKKRIGIF
                     KINSRNRRQYLMTTLLLNDKLELLKKSRIEIPMEERNEHLGDFSLDNEGDIIFSKFLR
                     TGNDNISSASFIIKKAAEDTLIVKSLNIEKTWLDEIHIKVDNYNKRYFLSSFYYKERR
                     GNIDGFYFYIWDKVGQQPIVENISIFSDELRKEARGDASMKTAFNDYFIRNIITRRDG
                     GFIIGSEAYYTTSRFNSWNRWDYLYGSPYLSPGFNNYYYSPYYDRFWWGGRSVNNQSV
                     RHHADNITVLSFSNTGQLEWSNVMGKSQFDDQSDELLSYQLMNTGGQIHFLFNQLERR
                     YNLLTDYSISPEGQMTRNPTLKNLDKGYEFMPKFGKQVSAKQMIMPCLYRNYICFAKI
                     DYN"
     gene            6756..7745
                     /locus_tag="PLO70_07325"
     CDS             6756..7745
                     /locus_tag="PLO70_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74311.1"
                     /translation="MIGIFKQKNPANIILLFVFGVLIKLPMFMHPHISEAREGDGILF
                     KGILTMLQSAGKSTPLIYPLLAYTLLFLQAIILTRFMAAQRMMSKSTYFPGMAYLLIT
                     SLFPEWNYFTAPLIVNTILLLVLSGLFNTYNHKSAKGAIFNIGLGLGIASFLFFPSLT
                     FIIWILLALMVMRPFRINELLLCLLGITAPFYFYAVYLFITDQWSWQNIWPQFTFHLP
                     RLQQSAWLAGSVFLLTVPFLMGGYYVQDNLRKMLIQVRKGWSLLLLYLLGALLVPFVN
                     NSATFENWVMAAIPFAAFHACTYLYSNLRIIPNLIFWLSVALVLAFQYTGPGW"
     gene            7878..8549
                     /gene="purQ"
                     /locus_tag="PLO70_07330"
     CDS             7878..8549
                     /gene="purQ"
                     /locus_tag="PLO70_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQZ74312.1"
                     /translation="MKFGVVVFPGSNCDRDMYDALSHDLGQEVSMLWHKDKDLSKFNT
                     EDCIILPGGFSYGDYLRCGAIARFSPMMQSVIEFANAGGKVFGVCNGFQILCESHLLP
                     GALLRNANQQFVCKNVFITNETGKTYKIPVAHGEGRYYADEKTLDQLEANGQVIFYYC
                     DENGNKTTEANPNGAIRNIAGICNANRNVFGMMPHPERACSPVLGNTDGQEIIKSLLM
                     AEQLV"
     gene            8702..9154
                     /locus_tag="PLO70_07335"
     CDS             8702..9154
                     /locus_tag="PLO70_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQZ74313.1"
                     /translation="MKRFLVTVLIFATAFTVQAQLNPISWTFTAKKIADKTFEIQMKA
                     TIQNKWHLYSQAQPADAIAIPTTFTFNPNPLFTLEGKVKEVGKLEKYHDKTLDVSANQ
                     YSNTVTFIQTIKLKANVKTSMTGNVEYQTCDDEKCLPPKTVPFTIAIK"
     gene            9230..11185
                     /locus_tag="PLO70_07340"
     CDS             9230..11185
                     /locus_tag="PLO70_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQZ74314.1"
                     /translation="MLRYLKPITAFFPILFSVLISVAQDSSAIKGWAVESKKLVDGKY
                     ELLFTVPVTDGWQLYAPNQVLLDVKTTELKFSDSSIAQEGEFILGSEPGKINSAIFET
                     SVSVFESSASWKAIIAINGTVPAKLQGTLFYTYGKNDEFYPSNPYSFTVTMEGGVEAA
                     SIKINSIDIHKPVNNCGDDGTKNKSLLTIFFLGLLGGLIALLTPCVFPMIPVTVTFFT
                     KKSTDKKKGITNAILYGLFIFLIYILITLPFHVAGKAISPEIFNNISTNVWLNLIFFA
                     IFVFFALSFFGLFEIGLPAGLANKMDSKSGLGNLGGIFFMAATLAIVSFSCTGPILGT
                     LLVGVADQGAWPLTVGAAGFGIALGLPFALFAMFPHWLQSLPKSGGWMTDVKVVLGFL
                     ELALAVKFLSNADLVKQWGLLKREVFIGLWVLIGILTVLYLLGKIRLSHSSPVKKFSF
                     IRIAFIILFTAITIYLLPGLTNTKWADLKLISGFPPPRTYSLFTIDKSKTGLFEPMHN
                     DYAGALERAKTENKPVLIDFTGWACVNCRRMEEKVWPDKTVDSLMRNEFVVVSLYVDE
                     RKNLPLAEQTVELLANGTEKSIVTVGDKWSTFQIENFGATSQPQYAIISPDQEAMTKT
                     KFYTPDADEFIKWLECGLDAFKKSKKQ"
     gene            complement(11343..12566)
                     /locus_tag="PLO70_07345"
     CDS             complement(11343..12566)
                     /locus_tag="PLO70_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ74315.1"
                     /translation="MKKNLLFLFLVILVVTTEKLKAQSSTTTLDVVSWNLEFFGAPYN
                     SGPPDKDLQEANAKKIMRYFDADIYGLVEIVDTVHLRKLRDSLGTNFEYIIAPYSTDG
                     PIGTNGWRQSQKLAFLYKKDLFSNINTRGMMLNSPTAVSNWANGRLPFLMKADVTING
                     ITKTVHFILIHGKAGSTASDYNRRLAGAQELKDTLDASFSTANVMIIGDYNDALTQTI
                     SIGSGPQSSYQPIVADSTDADHYKSITLPLGQAGQTSMINFPNVVDNHAISNEVVAYY
                     VLNSAQIRTDVTTVVPNYVTAHNTSDHYPVFSKYSLAGIITGLPVINPTEFGIRVSPN
                     PFNQTVTITATKSLNNVQIRLINMQGQVISSQLYGMMPAGSSFQPSFPDISKGMYFLQ
                     VETKQYRTVIKLIHL"
     gene            complement(12636..14336)
                     /gene="recJ"
                     /locus_tag="PLO70_07350"
     CDS             complement(12636..14336)
                     /gene="recJ"
                     /locus_tag="PLO70_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQZ74316.1"
                     /translation="MQKRWKILTADQQKVNVLQRSLKIHPVICKILIQRGIETFEQAK
                     DFFRPQLTALHSPWLMKDMDKAVERVTRAVNLKEKILVFGDYDVDGTTAVASMYQFLK
                     KIHSNLDFYIPHRYREGYGISKAGIDFAKANGFSLIISLDCGIKSADLIGYAKELAID
                     FIVCDHHLPDEILPPAVAILNPKQKDCNYPFKELCGCGVGFKLMCALSEKMQLPENAA
                     FEYLDLLATAIAADIVPVAGENRILAFHGLIKANKNPNYGIRALSKLSGLVKDLHITN
                     LVFMIAPKVNAAGRMDDARKAVQMFVANTEEEALLYAEQLHSDNTDRKEADKSITEEA
                     LALIHGKEEWIKRKSTLVFQPHWHKGVVGIVASRLIEHHYRPTIVLTQSGEYAAGSAR
                     SVPGFNLYEAIHACREHLLGYGGHFAAAGMTLELEKVDAFREKFEEIVSSTIQPELLI
                     PEIIIDAEISLKDIKQTFYDIISQMEPYGPENLRPVFITRNVINSGYSKIVKEEHIRF
                     SLREDNFIMNGIGFGMAGKFHLLDNQQPIDIVYTIDENEWNETKSLQLKVIDIQSTQP
                     "
     gene            complement(14375..15337)
                     /locus_tag="PLO70_07355"
     CDS             complement(14375..15337)
                     /locus_tag="PLO70_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQZ74317.1"
                     /translation="MQLPTDLIKPELKIFETRFKEAVRSQAPLLDRIMRYVVKRKGKQ
                     LRPMFVLLSAKLCGDINEPAYRAASLVELLHTATLVHDDVVDDSMERRGFFSPYALWK
                     AKANVLIGDYLLAKGLLLSLDNNDYQILHILSDAVRKMSEGELLQLEKARSLNLKEEV
                     YYEIIRNKTASLLASACAAGAWASSQDEAITEKMRLFGETTGMAFQIKDDLFDYASEN
                     VGKPTGNDIKEKKLTLPLIYTLNNTDKETRRKIIYIVKNNNQDKEKVKWVIDKVTAIG
                     GIAYATEKMNAYKKDALDILHQFHESPVRQGLEDMVLFVTDRKY"
     gene            15534..17261
                     /locus_tag="PLO70_07360"
     CDS             15534..17261
                     /locus_tag="PLO70_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ74318.1"
                     /translation="MASKKYLFLAGAFCLLASYGFSQRVGASYDVKDSSLVPSKRMPQ
                     HTEFLNGTYNFPSKPRNQWELGIKGGLFQVSGDVPAQFVSPGFGAHIRKAFGYIFSLR
                     LEYMYGIGKGYSFRANENYAKNPAWGANISDPNQRYSAPQAITGAGGVRQIVSSKTGL
                     TSTPFEQIFYNYKTKVQDLSLEGVVTLNNIRFHKSKTGFNFYGFAGIGGSIYDTKVNA
                     LDGSSKYNFNAINQAAYKNRKDFIKNMKNDVLDDSFETPAENQGDRRPKLFGDTFKPS
                     GTVGAGVAFKLSNRLNLAIEDRWTFIKDDLLDGQRWQEAAWGDATLTRDFDSYNYLSV
                     GLNINLGAKSVEPLWWLNPLDYAYSEIRNPRLMRLPKPVLPDTDGDGITDQFDREQTP
                     AGCPVDSHGVSLDTDGDGVPDCKDKELITPTYCQPVDADGVGKCPVPCPDEKCFEGFI
                     KQANDCATLLGALPSVSFKAGSNKLSDDTKAVLATVASKLRNNPGCKVVVVGYCSSSK
                     KEQQLSWDHVNAVINHMVDKEGISQDRFIFNYGQEGGDCNTVDLRAAGEGEEGPNRVE
                     PPHPNLRKN"
     gene            17427..18290
                     /gene="bamD"
                     /locus_tag="PLO70_07365"
     CDS             17427..18290
                     /gene="bamD"
                     /locus_tag="PLO70_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQZ74319.1"
                     /translation="MNYSFSRVIKPIHSFLSILSGIIFSVIILSSCGKGMNKLLKNPD
                     PAYKLRMAEQFFVKKQYTKAQQVYEDIMPYYKASKEFEDIYYKYAYCAYNLGDYMNAE
                     NLFKSYLEIFPNSTKAEEVDYMRAYSFYKQSPKPELDQTNTMRAMGMMQTFINTHPGS
                     PRNKEATDIIDVCRAKLESKDYKSAQLYYDLGQFRAAGVAFSALLNSYPESMRGDEYK
                     LMIIKSYYRFAELSIEEKKVERFEQVISECYEFTDRFPDSKLKKEAEIFLNLSQTNIK
                     NLNNEQTKTPA"
     gene            18265..18606
                     /locus_tag="PLO70_07370"
     CDS             18265..18606
                     /locus_tag="PLO70_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQZ74320.1"
                     /translation="MSKLRRQLSAGITNNVETRNLDEIKGKTGNLYESISIVGKRANQ
                     INISLKEELHNKLEEFASHTDSLEEIHENKEQIEISRAYERMPNPALLATQEFLDDKV
                     YFRKGEDELFS"
     gene            18729..19925
                     /gene="coaBC"
                     /locus_tag="PLO70_07375"
     CDS             18729..19925
                     /gene="coaBC"
                     /locus_tag="PLO70_07375"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQZ74321.1"
                     /translation="MLAGKKILVGITGSIAAYKSVYLVRLLIKAGAEVKVIMTPSAKD
                     FVSPLTLSTLSRNPVIIDLFDEQSWANHVMLGRWADVMVIAPLSCNTLAKMANGLCDN
                     LLLATYLSATCPVVAAPAMDEDMWHHPSTKANLVRLESFGNQIIPVEKGDLASGLNGE
                     GRMAEPERIIEYITTNFFLTRPLEGKKALVTAGPTYEPIDPVRFIGNHSSGKMGIAIA
                     KELYHRGAAVTLVMGPSQYEFSAEGIAVVRVKTADQMYKSCHESYPGSDLTVMAAAVA
                     DYTPVETADQKIKKKSDSLTITLSKTKDILKSLGEKKNSKQVLVGFALETNDEKKNAL
                     EKLAKKNADMIILNSMMDAGAGFGFDTNKITIFDKSGQEFSFDTKSKTDVAKDIVDTI
                     IRLYYA"
     gene            19918..20838
                     /locus_tag="PLO70_07380"
     CDS             19918..20838
                     /locus_tag="PLO70_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HQZ74322.1"
                     /translation="MHKRSLILFIFIFFMGFVQGQEIQARLSVITSKVSTQVDKKIFQ
                     TLQTGLTNFLNTRKWTTDVFQPSEKIQCSFLITIEQDMGNNIFKGKMTVQAARPAYST
                     TYDSPLMNFQDDNLVFKYQEFQPIEFNENRVQGNDPMVGNLTATLAFYVNMILGFDYD
                     SYSLRGGDMYFQKAWNIVNNAPDGRDITGWKSFESLRNRYWLAENMNNNRFALIHDAI
                     YSYYRSGIDVFFENEDAGRSGIINCLNFLNSINSENPNSMIMQFFFQGKANELVKIFS
                     KANAEQKSRAKELLTRLDITNTSLYNKELK"
     gene            20835..21275
                     /locus_tag="PLO70_07385"
     CDS             20835..21275
                     /locus_tag="PLO70_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HQZ74323.1"
                     /translation="MRIVILFFFILVAGSAGCKKKSSIPGNVLAQQKMQAVLWDMIRA
                     DHFINNYMVIKDSSLNKRVAGFKMYNRILGIHKISEEDFKKSFSFYRSNPELMWPIMD
                     SISKMSDEAPTQQIKPVPLEDSLQQTGPSTDTTSPRKIKPVSLD"
     gene            21341..22039
                     /locus_tag="PLO70_07390"
     CDS             21341..22039
                     /locus_tag="PLO70_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQZ74324.1"
                     /translation="MNKVVAGADEAIKDITDGVTIMLGGFGLCGIPENCIAALVKKGV
                     GNLTCISNNAGVDDFGIGLMLKQKQVKKMISSYVGENAEFERQLLSGELQVELIPQGT
                     LATRCLATGYGMPAIFTPAGVGTEVAEGKEVRQFNGKDYLLELAFEADFALVKAWKGD
                     TQGNLIYKETARNFNPLMAMAGKVTIAEVEILVPEGELDPDAVHTPGIYVHRIFQGTD
                     YEKRIEQRTVRKRN"
     gene            22108..22764
                     /locus_tag="PLO70_07395"
     CDS             22108..22764
                     /locus_tag="PLO70_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HQZ74325.1"
                     /translation="MALTKEQIAKRISRELKEGYYVNLGIGIPTLVANYIPEDMNVVL
                     QSENGLLGMGPFPLEGDEDPDLINAGKQTITTVPGSVFFDSAMSFGMIRAGKVDLTVL
                     GAMEVSDKGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMMHTNPKGESKLLPACTL
                     PLTGAGCVKKIVSDLAVLDVTPDGFKLLERAPGVSVEEIKAKTAGRLIVEGDIPEIEV
                     "
     gene            22863..23225
                     /locus_tag="PLO70_07400"
     CDS             22863..23225
                     /locus_tag="PLO70_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74326.1"
                     /translation="MIKAVADLDVFNISYALAMDIYHLAKNFPKEEKYSLTDQIIRSS
                     RSISANIAEGWGKRVYENEFRKYLIYSMGSLEETKVWLLFSRDCGYVSADVYTRISMK
                     CDETGAKIFKLYGNWKTF"
     gene            complement(23279..23929)
                     /locus_tag="PLO70_07405"
     CDS             complement(23279..23929)
                     /locus_tag="PLO70_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74327.1"
                     /translation="MGIIKVAIADDHKIFRKGVILSLRPYSNVKFVQEAENGEDLLNG
                     LAASEPDVVLMDLRMPGKDGIEATKTISKQYPQIKVLILSMYEDERFVYHLMENGANG
                     YLLKSAEPQEIRRAIMEVYEKGYYLNNFVNRILLKKSHSRQKVVPSLNSEITLSDKEK
                     DVLRFICMEFTAAEIAQKMEISPRTVEAIKDRLMERFGSKNTAGLVFFAVKNNLIE"
     gene            complement(23987..24814)
                     /locus_tag="PLO70_07410"
     CDS             complement(23987..24814)
                     /locus_tag="PLO70_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ74328.1"
                     /translation="MFLLQATNSSGVSFVLLLGTLGMLLLTVGLIIFIILHQRRVKRF
                     NEKIQTMEQAQQRLLLNASIKLQEEERQRLAADLHDDAGPLLATARLYLNENLVNQDK
                     ATQLQSIFQARQIIDDTIQLVRNISHSLMPPTLKNFGLESAINDLFQKISGSGAINAS
                     SRFHEYKERLKEEKELIIFRVVQELINNILKHSNSSFIHLTQNVHAGKFYLRIHHDGR
                     GLVQADFEKLNKSNVGLGLKNITSRLRVVDGNINFEKDISQTYYKVTIELPKDEPYE"
BASE COUNT         7269 a         4777 c         5317 g         7608 t
ORIGIN      
        1 ccggtacaga ttgttatggc tgcccccttc aatgctaatg agttcatctg ttgattttaa
       61 aaatggcaga aggcgtttcg aatttttgta agtaacgacc ctgtctttag ttccatgaaa
      121 aattgttatt ggtgctgtaa cattttgcag gtattgataa gttggtagct ggtaatgcag
      181 catccatttt gcaggatata tgggcaggta atgacttacc actgccggaa aatcataata
      241 aggagtttcc aggatcaatc gtttacaatc cctggtggat gcaagttgtg ctgcaatgcc
      301 tgttcccatg cttttcccat atatgattat actatcagca gggaatcgtt tacgggctat
      361 cttataaacc tgtaaggccc aatcgtacag tttttgttcg gtcagcttcc ctgtgctttt
      421 gccaaatcca gggtagtcga tcatcagcac ctggtagcca tgccgggtga aataaggaat
      481 gtatttagca taccaggaaa tatttctttt gttaccatga aaataaagaa ctatcccacg
      541 ggctatagta tctgtgctgg caaagtcaac caggttaaga gtgtcttttg aatcaatagg
      601 aatattgatg tctttatgag gctcaggaaa attataagaa taggttctgt tcaatgtaac
      661 cgggtgaaaa aggatggcat cctgtaggaa atataatgca accccgccag atatataaat
      721 gatagcgaca atctttgccc atttgattat tcttttcttg ttcatccggg caaagatgaa
      781 aagaaaaaag gacatgaagc tcatgtcctt ttagcgtaat gttttattag tattgattaa
      841 agtagctttt aatcctgtat agagcattgt tacttatttc atccagcagg cacctcatga
      901 tctcactttc cgtgggagca aactcccttc tgcgattgat cagctgttta tcattttcac
      961 ttaatgctct ttcatcgcct gtaaaagagg aaaattcagt actccagtta tggttggaag
     1021 aaaagttctc actccacagc cggcgaccgt tttcatctct cacatttacc tgcaacaggg
     1081 cagtagactg taacgttctt tttatggttg taatattagc aaatactctt gcatattctt
     1141 ttactattga gtcaggctta taaactgttt cctttacaac aatttcttta gacacttttc
     1201 ttgtgctcct gttgtcatag tagcggccga tatcaaggtt tgtaagacgc atgtctactt
     1261 ctttgtcaac ccttatatgc tggctccttg cgtcccaggc cgagtaaaat tttacaaatt
     1321 cattcccact gttgtattgc aggtttctga tcaactggtc atcaagatta ttgccaccaa
     1381 ctgtgtatga actatacaca tagccacctt gttgttgcat aggcagaatg actacattcg
     1441 taactgcata atcataagcc tcgttcattt tttgtatcac atcacggtta cctggtttga
     1501 attttaatgc tgcctggaac tcccggtacg cattgcgata gctttgttta tcataacgct
     1561 gcataaaggc aatacccctg tcatatcggg tatcaccggc tttttcacga taagtgacca
     1621 ggtaggagga gtagtcagca gggtttacaa tagtaaatac ctgtggcacc cggtaaatgg
     1681 catcatacat ccgttgaagc gatacatact cattatatat ccactcccat ttcagttcat
     1741 tggagctttc agcatttgcc ctgatgcggg tttcatggtc attcaccgca taccgatatg
     1801 aactctgaat gatgttgagg agctttgggt catcggggtc tttctgtaat ttttttgcag
     1861 ccagttccac ggcttctgta taattgcctt tttcatatag tttactggca gttttacagg
     1921 agactgctga aatgctgatc ccggttaaaa gtagaatgta gagttttagt ttcataaaca
     1981 cctccgtttt catgtagtac gttcaaattg cagtccagtt aacagcaggt gctgtcaatt
     2041 aagcaatcat taacaattga tgggcaatac gtctagtatt tgagaatgtc cctgatgaaa
     2101 ttgtggtact catcaaacga tggcaaattg ttgtgtcctc ctccatgaat gcgtatgagg
     2161 gtgatcttat gctggtttat cttctgtaaa gcttcgctat gccggattgg tatcagccag
     2221 tcttttgtac catgtataat gtaagtgtga cactttacat taggtaacca tttatcggta
     2281 cgaaggtgaa agcgaaggac gatgcgaacg ggtagaatag gtaaaaaacg ttcaattacc
     2341 ctcaggaaat taaaatacgg ggcatccaga atcaggtacc gggggctatt gtccgaggca
     2401 agttttgttg cgaagccact acccatgctg cgaccatata cgatcaggtg atcctctgca
     2461 tacttttccc ttagtttatt gtaaataaac tgcatgtcat tcagcatttg cttttcaccc
     2521 cttttgccag tgctcttgcc gaatccccgg taatcaagca aaagcacatc atagtcgtac
     2581 cggtaaaaat ctttggcata ccgtgcccag ccttttatac ttcttgtatt gccatgaaaa
     2641 tataatatca aacctttggg attttcccgg aagaagtgga gaccgttaat gcgtacaccc
     2701 ggttcaatgt caaaaaagta ttctctgaaa ggcgcatcat acttaaactg aaaatcctgt
     2761 ttcagctttt ccggtttaaa tatcagtctt tcctgtatga agtaagcaat gacggacagt
     2821 gctgcaatgc caataatgat ataccagatg aagacaggca agctagtttt agtttatgca
     2881 ctgaaattaa ggaacatgcc acaaactgta aactttctta ttcttctact ctttttagtt
     2941 ttaaattagt aacaggcgct acaattaatg ggaatttctc taccgtaaca tttgcaaggt
     3001 caagtccaaa acctttttct tctgatagac tatgatcttt cagccagaag tggaatttca
     3061 taattactct ttcaaagaat ggaagttcat tgtcctggct caggtatttt tccattacca
     3121 taaactggaa gtcaccaact gtattactgc tggccaggct ttcataccgg ctgataatat
     3181 tcacttcttt attggctaca agatcttcaa ctactttacg gaacataaga tttacccgtg
     3241 gctccatcct gaatccaaga cggaaatcaa ccctgataat atcatttgga atgatatgtt
     3301 ctactttgta ttcagaagta tatgggtcat ctacggtatc aatgtgtacg aaccagtaaa
     3361 tgtcagcccg tttgggtttt ctattcagta tagaataaat gatcttatgc tcaatctctt
     3421 ttgggttgtt ggcactggtc atataaacca ggtgagtggc atatttggga acagacttgt
     3481 cattactaag ctcttgtatc ttgggtatat aatgttctag gcgaacaaat tccacataac
     3541 ggttcttgat tttccttgcc ctgtaccaaa tgtacataac aagaaatacc agcataccaa
     3601 tgatgagtgt tatataacct ccgtgagtga attttaccat taatgcgaca aggtatccga
     3661 tttccacaat caggtaacca ccaagaaaaa tatagatcca gatagatttt gcccggtgca
     3721 gaaccaggta attcgcaaaa agaatggtag tggcaagcat ggttacaata atcgcaagcc
     3781 catatgctgc ttccatgtta gaggatttac ggaagtacaa tacaattccg gcacaaccca
     3841 aaaacatcag aaaattaata gtgggtataa acaactgccc ttttgcttcg gaaggataac
     3901 ggatcctgag ttttggccat aaattaagcc gcatggcttc acttatcagt gtgtaagcac
     3961 ctgaaaccat agcctggctg gcgattattg cagccgtagt tgcaataatt actcccggta
     4021 taataaacca atggggcatc aggtcataaa aggcattaat gcttaatgaa ctctttactt
     4081 cgctggtaat ttccaggcca cgtcggtgtg ccagcaaata ggcaccctgt ccaaaataat
     4141 ggatcagcag gcatgttttt acgtaaatcc atgatgagcg gatatttccc ctgccacaat
     4201 ggcccaaatc actatataag gcttcagccc cggtagtaca aagaaaaacg gcacctaaaa
     4261 gccaaaaccc ttctggataa tttttcagaa attcaaaagc ataatagggg cttagtgctt
     4321 taaaaatggc caggtcatca aacacatgta taatacccag tatcagcagc atcgtgaacc
     4381 agatgaacat tattggtccg aataatttac cgatagaggc agttccaaat tgctgcatga
     4441 aaaagaaaag tgccaggata gaaagaacaa taattacaac tgtattgctg ccgtcctcaa
     4501 ttcccaaaga aggtagttct tttaatcctt caatagcaga ggtaattgag atgggcggag
     4561 tgatgatgcc atctgccaat aacgatgccc ctccaaccat ggcgggaata accaaccact
     4621 ttttacggcg cctcactaag gcatataaag caaaagtgcc gccttccccc cggttgtcag
     4681 cccgcaatac cagtatgaca tatttgatgg tggtttgaag tgttaatgtc catataatgc
     4741 aggatagggt gccaacgatc agttcctcag taaccaccct attaccaata attgcattga
     4801 aaacatatag cggggacgtt ccgatatctc cgtaaataat tccaagggca ataagtaatc
     4861 ctgcaccggt aactttattt gtcgatactt tcactttttg gtagttgtgg tttgtttcct
     4921 ggggtgcccg ctatagcccc ggatagccct ggatataaac gggtacagat tttgcacttg
     4981 tgtgaacatt cttacaaagt tataggcaaa attcaattac aaccagcaaa ccggggaact
     5041 ttacttttcg tcttcatgaa gtgacaggaa agatcctgta gtttaaaaca actggtatat
     5101 accggttgtt gtacatgtaa tagtcaacaa aactgcatca acaaaaatac aacagcctgt
     5161 tttggctgct aacttgtaac tttaatatgg aaaaactatg tgtacaatgt ttggtaaaaa
     5221 atggataatt tctgtactgg caatgagtat gataatgccg gctacggctc aaaaaatcat
     5281 ttattccgac ccggataagg atgatacccg caggctgaat tttgaaatag ttggcaaaat
     5341 aaacggcaat tttctgatct ataaatatgt caggaataaa agctggatca cggtactgga
     5401 taacgatatg aagcaaattg cccgggaaga acaggattat gtaccggcta atgacagggt
     5461 gataaatgtc gattttttcc catatagtga tcatgcttac atggtctacc agtaccagaa
     5521 aaggaatgtt gtctactgta tggcctccag gatcgatgga aatggaaaga aaaccgggga
     5581 tgtgatcgca ctggacacta ctcatatcgg ttttgcagcc aataacaaaa tctactctgt
     5641 tctgagcagt gaagacaaaa aaagaattgg catatttaaa attaatagcc gaaacagaag
     5701 gcaatattta atgacaacat tactgttgaa tgacaagctt gagttgctta aaaaaagccg
     5761 tatagagatt ccaatggaag agaggaatga acacctcggc gattttagtc tcgacaatga
     5821 aggcgatatc atattttcta aattcttaag gaccggcaat gataatatta gcagtgcttc
     5881 attcattatt aaaaaagcag ccgaagatac tttgatagtg aagtcgctga atattgaaaa
     5941 aacctggctg gatgagatcc atataaaagt ggataattac aataaacgat attttctatc
     6001 ctctttttac tacaaagaga gaagagggaa tattgacggc ttttattttt atatctggga
     6061 taaagtaggc cagcagccaa ttgttgagaa tattagtatt ttttcagatg aacttcggaa
     6121 agaggccagg ggcgatgcca gtatgaaaac cgcttttaat gattatttta tcaggaacat
     6181 tattacccgc agagacgggg gctttataat aggcagtgaa gcgtattata ctacttcccg
     6241 gtttaatagc tggaaccggt gggattactt gtatgggtct ccttatttat cacccgggtt
     6301 taataattat tactattctc cgtattatga tcgtttctgg tggggaggcc gatctgtgaa
     6361 taaccagtct gtacgccacc atgccgacaa tattacagta ctttctttca gtaataccgg
     6421 gcagttggag tggagtaatg taatgggcaa aagccagttt gatgatcagt ccgacgaact
     6481 gctttcctac cagctaatga atacgggtgg ccagatccat tttcttttta accaactgga
     6541 aagaagatat aatttattaa ctgactacag catttcacct gaaggacaga tgacaagaaa
     6601 tcctacatta aaaaatcttg acaaaggata cgagtttatg ccaaaatttg gcaaacaggt
     6661 cagtgccaaa caaatgataa tgccttgttt atatcgtaac tatatttgtt ttgcaaaaat
     6721 cgattataac taacccgtaa ttctgtccat tatctgtgat tggcatattt aaacagaaaa
     6781 acccggccaa cattatcctg ctgtttgtct ttggagtgtt gattaaactt cccatgttca
     6841 tgcatccgca tatcagcgag gcaagggaag gagacggcat tctttttaaa gggatactga
     6901 caatgctgca atctgccgga aagtcaacgc cgcttattta tcccttgctg gcatatacct
     6961 tgctgttttt gcaggcaatt atcctgaccc ggtttatggc tgcacaaaga atgatgagca
     7021 aatccacata ttttccgggt atggcatacc tgcttattac ttctttattt ccggagtgga
     7081 attattttac tgcaccgttg attgtaaata caatattact actggtactt tcaggtctgt
     7141 ttaatacgta taatcataaa agtgcaaaag gagcgatctt taatatcggt cttggtttag
     7201 gcattgcatc atttcttttt ttcccctccc ttacttttat catttggatt ctactggctt
     7261 tgatggtgat gaggccattc agaattaatg aattgttact ttgcctgttg ggtattaccg
     7321 ccccttttta tttttatgca gtgtacctgt ttattaccga ccagtggagt tggcaaaata
     7381 tatggccaca gtttactttt catcttcccc gactgcagca atctgcctgg ctcgccggca
     7441 gtgttttttt attgacagta ccttttttga tgggagggta ttatgtacag gataaccttc
     7501 gtaaaatgct gattcaggtg cgtaaaggct ggagtttgct actgttatac ctgttagggg
     7561 cgttgttggt tccttttgtc aataatagtg caacttttga aaattgggta atggcagcaa
     7621 tcccttttgc ggcttttcat gcctgcactt acttatattc caatctccgg atcataccaa
     7681 atttgatctt ctggttatca gtagcacttg tgctggcttt ccagtatact ggtcctggct
     7741 ggtagtcaaa aggatgcaag ccactggcta tttgcaggta tactttatcc cgggtatttc
     7801 aggttctaag tcgtagctta tggagggcag cctttcttaa aatcttatat ttgctttttc
     7861 ttatttaata ttcctttatg aaatttggtg ttgtagtttt tccaggctct aattgcgata
     7921 gagatatgta tgatgctttg agtcatgatc ttggacagga ggtgtcaatg ctatggcata
     7981 aggataaaga tttaagtaaa ttcaatacgg aagattgcat catccttccc ggtggttttt
     8041 cgtatgggga ttacctcaga tgtggtgcta ttgcccgttt cagcccgatg atgcagagtg
     8101 tgatcgagtt tgccaatgct ggtggtaaag tgtttggagt ttgtaatggt tttcagatcc
     8161 tttgcgaatc acatcttttg ccgggtgctt tattacggaa tgccaaccag caatttgtat
     8221 gtaagaatgt atttatcacg aacgaaactg gtaaaacgta taagatcccg gtagcacatg
     8281 gtgaaggccg gtactatgcg gatgaaaaaa cattggatca actggaggct aatggccagg
     8341 taatatttta ttactgtgat gaaaatggca ataaaacaac tgaagcaaat cctaacggag
     8401 ccatccgtaa tatcgccggt atttgcaatg cgaaccggaa tgtatttggg atgatgccgc
     8461 acccggagag agcctgctct cccgttttgg gcaatacaga cgggcaggaa ataataaaaa
     8521 gcctgctgat ggcggagcaa cttgtttagt ctcaggtgca tcttgtcatt gctcgaccgg
     8581 cgtttgtcac ttttatcaca ttccggacta aaccagaagg ttacttttgg ggcagagatt
     8641 ttaacagatt gagtaaagaa aaagcaggat ttttgtaccc agataattta aacaattgag
     8701 aatgaaaaga tttttagtca cggtattgat ttttgcaaca gcttttactg ttcaggccca
     8761 gttaaacccg atctcatgga catttacagc taagaaaatt gctgataaga cgtttgagat
     8821 tcaaatgaaa gccactatcc agaataaatg gcacttgtat tcccaggcac agccggctga
     8881 tgccatcgct attcctacaa catttacatt caatcccaat cccctgttta cactcgaagg
     8941 aaaagtaaaa gaagtcggaa agcttgagaa ataccatgac aaaacactgg atgtgtccgc
     9001 taaccagtat tccaataccg taacttttat acaaaccatt aaattaaagg cgaatgtaaa
     9061 aaccagcatg accggtaatg tggaatacca gacctgtgat gatgaaaagt gtttgccgcc
     9121 aaaaacagtt ccctttacga tcgcaatcaa ataaatagtg aattaatatt tttgatatta
     9181 ataacgggtt ctcttaaaga actcgtttat tttttaaatg tatgctgcta tgctccgcta
     9241 tttaaaacca ataactgcct ttttccctat acttttttcg gtacttattt ctgttgctca
     9301 ggattcttct gctattaaag gctgggccgt tgaaagtaaa aaattggtag acggaaaata
     9361 tgagctcttg tttacagtac cagttacaga tggttggcaa ttgtatgctc ctaaccaggt
     9421 actgctggat gttaaaacaa ccgagttgaa attctctgat tcaagtattg cgcaggaagg
     9481 tgaatttata ttgggaagtg aacccggcaa gattaatagt gccatatttg agacatccgt
     9541 aagtgtattt gaaagtagtg cttcctggaa agcgatcatt gctattaatg gaacagttcc
     9601 ggcaaaattg cagggtacct tattttatac ttatggtaag aatgatgagt tctatccatc
     9661 aaacccttat tccttcacag ttactatgga aggaggggta gaagcagcca gcatcaagat
     9721 taattcgatt gatattcaca aaccagtaaa caattgcggc gatgatggta caaaaaataa
     9781 aagcctgctt actattttct ttttaggatt actgggtggc cttattgcat tgcttactcc
     9841 ctgtgttttt ccaatgatcc ccgttactgt taccttcttt accaaaaagt ctactgataa
     9901 aaagaaaggg attactaatg ccattctgta tggacttttt atttttctga tttacatcct
     9961 tattactctt cctttccatg ttgccggtaa agcgataagt cctgaaatat tcaataatat
    10021 atcaaccaat gtctggctca atcttatctt ttttgccatt ttcgtttttt tcgctttatc
    10081 attttttggt ctttttgaaa ttggtcttcc tgcaggattg gctaacaaaa tggattccaa
    10141 atctggtctt ggaaatttag gtgggatctt ctttatggca gctacactgg ccattgtatc
    10201 attttcatgt acaggcccaa ttttaggaac attactcgtc ggcgttgccg accagggtgc
    10261 ctggccgctt actgttggtg cagcaggttt tggtatagct cttggcttac ctttcgcttt
    10321 gtttgccatg tttccgcatt ggttacaatc gttgccgaaa tctggtggat ggatgacaga
    10381 tgtgaaagtg gttttgggtt tcctggagct ggctttagcc gtgaaattcc tatcaaatgc
    10441 tgacctggtt aagcaatggg gcttattgaa aagggaagta tttattggtc tctgggtgct
    10501 gataggaatc ctcaccgttt tatatctcct gggcaaaatt cgtttatcac acagttcgcc
    10561 ggtaaagaaa ttttcattta tacgaattgc ttttataatt ctgtttacgg caataacaat
    10621 ttacctgtta ccgggtctta ccaatacaaa atgggctgat ctgaaactga tcagtggttt
    10681 ccctccgcca agaacatata gcttatttac gattgacaaa agtaaaacag gacttttcga
    10741 acccatgcac aatgattatg caggtgcatt ggaacgggcc aaaaccgaga ataaaccggt
    10801 gttgattgac ttcaccggtt gggcctgtgt gaactgccgt cgtatggaag aaaaagtatg
    10861 gcccgataaa accgttgata gtctgatgcg aaatgaattt gtcgtggtct ccttatatgt
    10921 ggatgaaaga aagaatttac cactggcaga acaaacagtg gaactgttgg ctaacggaac
    10981 agaaaaatca attgtaactg ttggcgataa atggtccaca ttccagattg aaaattttgg
    11041 tgccacatct cagccgcagt atgcaattat cagcccggat caggaggcta tgacaaaaac
    11101 aaaattttat actcccgatg ctgacgaatt tattaaatgg ctggaatgtg gtctggatgc
    11161 atttaagaaa agtaaaaagc agtagctatt attattaaaa aagaagaggc tgtctcaaat
    11221 gctccaatca atgtcatatt gagcaaagcc gaaatatgac attgattggt aaacaaattt
    11281 gtacgcattt cgatatgctc aatgtgacaa gtttgtttgc ttttgagact gcctcttatt
    11341 ttttacaaat gaattagttt aataaccgtt ctatattgtt ttgtttccac ctgcaggaaa
    11401 tacatacctt ttgaaatatc cgggaatgaa ggctggaaag aagatccggc tggcatcatg
    11461 ccatacaatt gagagctgat aacctgccct tgcatattga taagcctgat ctgaacatta
    11521 ttcaatgatt ttgttgcagt aatagtgaca gtttgattga aaggattggg tgaaaccctg
    11581 atcccaaatt cagttggatt aattaccggc aagcctgtaa taatacctgc gagactatat
    11641 tttgaaaaaa cagggtagtg gtccgaagta ttgtgggctg taacataatt cgggacaaca
    11701 gttgttacat ctgttcttat ctgcgccgaa ttaagaacat aataggctac tacttcattg
    11761 gagatggcat gattatctac aacattagga aagtttatca tactggtctg ccccgcctgg
    11821 cctagtggta aggtgataga tttataatga tctgcatcag tagaatctgc aacaattggc
    11881 tggtaagagg attgtggtcc cgatccgatg gatatggttt gagtcagcgc atcattatag
    11941 tccccgatga tcataacatt ggcagtggaa aaagaagcat ccagtgtatc tttcaactcc
    12001 tgggccccgg ccaaccgcct gttataatca gatgccgttg acccggcttt gccatgtatc
    12061 aaaatgaaat ggaccgtttt tgttattcca tttattgtaa catccgcttt catcaggaaa
    12121 ggcagcctcc catttgccca gttagaaaca gcagttgggc tattcagcat cattcccctg
    12181 gtgttaatat tgctgaataa gtctttcttg tacaagaaag ccaacttctg gctttgtctc
    12241 cagccattgg taccaatcgg gccatcagta ctgtatggcg caattatgta ttcaaaattt
    12301 gttcctaaag agtctcttaa ttttctgagg tgtacagtgt ctactatttc aaccaggcca
    12361 taaatgtcgg cgtcaaagta gcgcataatt ttttttgcat tggcttcctg caggtcttta
    12421 tccggaggtc cgctgttata gggggctccg aagaattcaa gattccagct aactacgtca
    12481 agtgtagtgg tactgctctg tgcttttaat ttttctgttg ttacaaccag aattaccagg
    12541 aataaaaaga gtaaattttt tttcatgttc tcattttcgt agacaaatgt aagggagaaa
    12601 aataaggtaa agtcaggcta ttatgaaaag aactgtcacg gttgtgttga ctgtatatca
    12661 ataactttca gttgcagcga tttggtctca ttccattcgt tttcatcaat tgtatacacg
    12721 atatctattg gctgctggtt atccaaaagg tgaaatttac cagccatccc aaagccaatt
    12781 ccattcatga taaaattatc ttcccgcagg gaaaatcgta tatgttcttc ttttacaatt
    12841 tttgaataac cggaattaat aacattcctg gtgatgaata ccgggcggag attctccggt
    12901 ccgtatggtt ccatctggct aatgatatcg tagaaagttt gtttgatgtc ttttaatgaa
    12961 atttctgcat caataatgat ttccggaatt agcagttccg gttgaatagt ggatgatacg
    13021 atctcttcga atttttcccg gaaagcatcc actttttcta attctaacgt cataccagcc
    13081 gctgcaaaat ggccaccata gccaagcaag tgttccctgc aggcatggat agcttcatac
    13141 aaattaaaac cgggaacact ccttgcactg cccgccgcat attcgccgga ctgtgtaagc
    13201 acaatagttg gacgataatg atgttctatt aaccgggatg ccactattcc aactactcct
    13261 ttatgccagt gaggttgaaa aactagagtt gactttcttt ttatccattc ttcttttcca
    13321 tgtattagag ctaacgcttc ctctgttatg cttttatcag cttcctttct gtcggtgtta
    13381 tcactatgca gctgttcagc atatagtagg gcctcctctt ctgtgttcgc tacaaacatc
    13441 tgtacagcct ttctggcatc atccatcctg ccggcagcat ttacttttgg cgcaatcata
    13501 aataccaggt tggtaatatg gaggtccttt accaggccgc ttaattttga taaagccctg
    13561 atgccgtaat ttggattttt atttgccttt atcaagccat ggaaagctaa tatcctgttt
    13621 tccccggcaa caggtactat gtctgctgca attgcagtcg ccaggagatc aagatattcg
    13681 aatgctgcat tttcaggcag ttgcattttt tctgacaggg cgcacataag cttgaagccg
    13741 actccacagc cgcaaagttc tttaaaagga taattacagt ctttttgttt tggattaaga
    13801 attgcaacag caggtggtaa tatttcatca ggtaaatggt gatcgcaaac aataaaatct
    13861 atagccagtt cttttgcata accgattaag tctgctgact taataccaca atcaagagaa
    13921 ataattaaag aaaacccatt tgctttcgca aaatcaattc ccgccttact gatgccataa
    13981 ccttcccggt aacggtgggg gatgtaaaaa tcaaggttgg agtgaatctt cttaagaaac
    14041 tggtacatac tggcaacggc agttgttccg tctacatcat agtctccaaa aacaagaatt
    14101 ttttccttta ggttaactgc cctggtaacc cgttccacag ctttatccat atccttcatc
    14161 agccacgggc tatgcaatgc cgttagttgg ggccggaaaa aatcttttgc ctgctcaaat
    14221 gtttcaatgc cccgttgtat cagaattttg caaataaccg gatgtatttt cagtgaccgt
    14281 tgtaatacat tcactttttg ctggtcagct gttaatattt tccatctttt ttgcattacc
    14341 aatcaatgat gaattatatg tttaaggtaa aaaattaata tttacggtca gttacaaaca
    14401 ggaccatatc ttcaagtcct tgcctgacag ggctttcatg gaattgatgc aggatgtcaa
    14461 gggcgtcttt cttgtaggca ttcatttttt cagttgcata ggcaattcca ccaattgccg
    14521 tcactttatc tattacccat tttacttttt ctttatcctg gttgttattt tttacgatgt
    14581 aaataatctt tcgccgggtc tctttatccg tattattcag ggtatagatt aaggggagag
    14641 tcaatttctt ttcctttata tcatttccgg tgggcttgcc tacattttca ctggcataat
    14701 caaataaatc gtctttaatc tgaaatgcca tgccggttgt ttcaccaaac aatctcattt
    14761 tttctgttat tgcttcgtcc tggctggaag cccatgctcc tgccgcacag gcagatgcca
    14821 ggagggaagc agttttattc cggattatct cgtaataaac ctcttctttc aggttcaggc
    14881 ttcgggcttt ttccagttgc agcaattctc cttcactcat tttgcggacc gcatcagaaa
    14941 ggatatggag gatttggtaa tcattattat cgagagaaag caggagtcct ttagccagta
    15001 aatagtcgcc aataagtaca ttggcttttg ctttccaaag ggcataagga gaaaaaaaac
    15061 cccttctttc cattgaatca tcaactacgt cgtcatgtac aagtgtcgca gtatgaagca
    15121 gttctaccag ggatgcggcc ctgtatgccg gttcgttgat atcaccacaa agtttggcag
    15181 aaagtaacac aaacatgggt cggagttgtt tacccttacg tttcaccaca tacctcataa
    15241 tgcggtctaa aaggggtgct tggctgcgaa cagcttcttt gaatcgtgtt tcaaagattt
    15301 ttaattcggg ttttatcagg tcagtgggta gttgcattgc caaaatcagg gcaatttacg
    15361 ggcaaaaaaa taattgtcag cagatgcaac aaaatacact caaaaattga ctaagtaatt
    15421 gaaaaataat ggacaaaagg gcaaataatt gtcataaaaa tttggtaatt aacgcccatt
    15481 atctattttt gcgaaatatt taggacattg ttcacaaatc ttaatcctta gttatggcaa
    15541 gcaaaaagta tttatttctg gcaggcgcat tttgcctgtt agcgtcttat ggtttctcgc
    15601 aaagggtcgg ggccagttat gacgtaaaag attcttccct ggttccgagt aaaaggatgc
    15661 ctcagcacac tgagttcctt aatggtacat ataatttccc ttcaaagccc cgcaatcagt
    15721 gggaacttgg tattaaaggt ggtttgttcc aggtaagtgg tgatgttccg gcacaatttg
    15781 tatctccggg ttttggagca cacatcagaa aagcatttgg ttatatcttt tctttacgtc
    15841 ttgaatacat gtatggaata ggtaaagggt atagtttcag agccaatgaa aactatgcta
    15901 agaatccagc atggggtgca aatatttctg atcccaacca gcgttattcg gctccacagg
    15961 cgattacagg cgcaggcggt gtacgccaga ttgtctcttc taaaactggt ttaacaagta
    16021 ctccttttga gcaaattttt tacaactaca aaacaaaagt gcaggattta agccttgaag
    16081 gagttgtaac gttaaataat attcgtttcc acaaatctaa aacaggattt aatttttacg
    16141 gatttgccgg tatcggcggt agtatctatg ataccaaagt gaatgcctta gatggatctt
    16201 ctaaatataa ttttaatgct atcaaccagg cagcctataa aaatcgtaaa gattttatca
    16261 agaatatgaa gaatgatgtt cttgatgata gttttgaaac tccggcagaa aaccagggtg
    16321 atcgtcgtcc aaaattgttt ggcgatactt ttaagccttc cggtacagta ggtgcaggtg
    16381 ttgcatttaa attgagcaac aggttgaatc ttgctattga agaccgttgg acttttatca
    16441 aagatgattt gctggatgga cagcgttggc aggaagcagc ctggggtgat gctactttaa
    16501 cccgtgattt cgattcttat aattacctga gcgttggttt gaatatcaac ctgggtgcaa
    16561 aatcagttga gccactatgg tggttaaatc cgcttgatta tgcttacagt gaaatcagga
    16621 atccccgttt gatgcgttta ccaaagccag tattacctga tactgatggt gatggtatta
    16681 cagatcagtt tgaccgtgag cagacacctg caggttgtcc tgtggattct catggtgtaa
    16741 gtcttgatac tgatggtgat ggtgttcctg attgtaagga taaagaactg atcactccta
    16801 catattgcca gcctgttgat gcagacggcg taggtaaatg ccctgttcct tgccctgatg
    16861 aaaaatgttt tgaaggtttc attaagcaag ctaatgattg tgctacttta ctcggtgcat
    16921 taccaagtgt atcttttaaa gcaggttcta ataaactcag cgatgatacc aaagctgttt
    16981 tagcaacagt agcttctaaa ttgcgtaata atccaggttg taaagtagta gtagtaggtt
    17041 attgctcttc tagtaaaaaa gagcagcagt taagctggga tcatgtaaat gcagttatca
    17101 atcacatggt tgataaagaa ggcattagcc aggacagatt tatcttcaac tacggccagg
    17161 aaggcggcga ttgcaatact gttgatcttc gtgctgctgg tgaaggtgaa gaaggaccaa
    17221 acagggtaga acctccacat cctaatcttc gcaagaacta atatttcata ctttttgcta
    17281 aataggccct cccccgatta atcaggggag ggtttttttt aatgggggta tagctaccag
    17341 ctttataata accttaactt caagggtttg gctgcttatt ttttttacgt aagttttgcc
    17401 ggtatcagtt aactttgcac tcctttatga attactcttt ttcccgtgtc ataaaaccca
    17461 ttcacagttt ccttagcatt ttatcaggga tcattttctc agttataatc ttaagtagct
    17521 gtgggaaggg aatgaataaa ttactgaaga accctgaccc ggcttataaa ttgcggatgg
    17581 cagagcagtt ctttgttaaa aagcaataca caaaggcgca gcaggtgtat gaagatataa
    17641 tgccctatta taaggcaagt aaagagtttg aggatatata ttacaagtac gcatattgcg
    17701 cctataacct gggtgactac atgaatgctg aaaacttatt caaaagctat cttgaaattt
    17761 ttccaaacag taccaaagca gaagaagttg attacatgcg ggcttattct ttttacaaac
    17821 aatcccctaa gccagaactt gatcaaacca atacaatgag ggctatgggt atgatgcaga
    17881 cctttatcaa tactcatccc ggatcgcccc gcaataaaga agctactgat atcatagatg
    17941 tttgcagggc aaagcttgaa tcgaaagatt ataaaagtgc acagctatat tacgaccttg
    18001 ggcagttcag ggcagcagga gtggcattca gtgctttatt gaattcgtat cctgagtcaa
    18061 tgcgtggaga tgagtataag ctgatgatca ttaagtccta ttatcgtttt gcagaactaa
    18121 gtatagaaga aaagaaagtg gagcggtttg aacaggtgat aagtgaatgc tatgagttta
    18181 ctgacagatt tcctgatagt aaattgaaaa aagaagctga aatattttta aatttatctc
    18241 aaactaatat taaaaactta aataatgagc aaactaagac gccagcttag cgcaggtatc
    18301 accaacaatg tggaaacccg taacctggat gaaataaaag ggaagaccgg taatctttat
    18361 gaatccattt ccattgtcgg aaaaagggct aaccagatta atatttcgtt gaaggaagaa
    18421 ttgcataaca agctggaaga atttgccagt cataccgaca gcctggaaga aattcatgag
    18481 aataaggagc aaattgagat ttcccgtgca tacgagagaa tgcccaaccc tgccttgttg
    18541 gctacacagg aattcctgga tgataaagtg tatttccgta aaggagaaga tgagctgttc
    18601 agctaaaacg gtaaaactaa atttttaaga atttctaaag cgatgggttc ccgtcgcttt
    18661 ttatatttaa cagctatttg cttaatcagc tatgagcttt tacaagtaaa tggtactttt
    18721 gataaccaat gttggcaggt aaaaaaatat tagtgggaat tactggcagt atagctgctt
    18781 ataaatctgt ttacctcgtc aggttactta tcaaggcagg tgcagaagta aaagtaatca
    18841 tgacaccatc tgccaaagac tttgtttctc cgcttacctt atccaccctg agccgtaacc
    18901 cggttattat agatcttttc gatgaacaaa gctgggccaa tcatgtgatg ctcggtcgct
    18961 gggcagatgt aatggtgata gctcccctaa gctgcaatac attggcaaag atggctaatg
    19021 ggctatgtga taaccttctg ctggctactt acctgtcagc tacatgtccg gttgttgcag
    19081 ccccagccat ggatgaagat atgtggcatc atccctccac aaaagccaac ctggttaggc
    19141 tggaaagttt cggcaatcag atcattccgg tagaaaaggg agaccttgca agtgggttga
    19201 atggtgaagg caggatggca gaacctgagc ggatcattga gtatatcact actaattttt
    19261 ttttgacccg gccacttgaa gggaaaaaag cattagtgac tgcagggccc acatacgaac
    19321 caatcgaccc ggttcgtttt ataggcaatc attcatctgg taaaatgggg attgctattg
    19381 caaaggaatt atatcatcgt ggtgcagcgg taacactggt aatgggacct tcacaatacg
    19441 agttttcagc agaagggata gctgtagtga gagtgaaaac agcagaccag atgtataaat
    19501 cctgtcatga aagttacccg ggatctgacc ttactgtaat ggcagctgca gtggctgatt
    19561 ataccccggt ggaaacagct gatcagaaga tcaaaaaaaa atccgactct cttacgataa
    19621 ccttatccaa aacaaaggat attttgaaaa gcctgggtga aaagaaaaat agtaaacagg
    19681 tactcgtggg ctttgcactg gaaacaaatg atgagaagaa aaatgcactg gaaaagcttg
    19741 ctaagaaaaa tgcagatatg atcattttaa attccatgat ggatgccggt gccggttttg
    19801 gttttgacac taataagatc actattttcg ataaaagcgg gcaggaattc agctttgata
    19861 ccaaatcgaa aaccgatgtg gcaaaagata ttgttgacac tataatccgt ctgtattatg
    19921 cataaaagaa gtctgatact ttttatattc atttttttta tggggttcgt gcagggccag
    19981 gagatacagg cccgtttgtc agtcatcacc agtaaagtga gtacccaggt tgacaaaaaa
    20041 atatttcaaa ctttacagac agggctcact aattttctga atacccgtaa atggacaact
    20101 gatgtcttcc agccttcaga aaagattcag tgtagttttc ttatcactat tgagcaggat
    20161 atgggtaata atatctttaa aggaaaaatg acagttcagg cagcccggcc ggcttatagt
    20221 actacctacg actccccgct tatgaatttc caggatgata atctcgtttt taagtaccag
    20281 gagtttcagc ccattgaatt taatgagaac agggtgcagg gtaatgatcc gatggtgggt
    20341 aaccttaccg ctacacttgc tttttatgtc aatatgatcc tcgggtttga ttatgattcg
    20401 tattcacttc gtggaggtga tatgtatttt caaaaagcat ggaatattgt aaataatgct
    20461 ccggacggca gggatattac tggctggaaa tcttttgaga gtctgcggaa caggtattgg
    20521 ctggcggaaa atatgaataa taaccgcttt gcgcttattc atgatgctat ttattcttac
    20581 taccggtcag ggatcgatgt tttttttgag aatgaagatg ccggccgtag cggtatcatc
    20641 aattgtttaa actttctcaa ctctattaat agtgagaacc ccaattctat gatcatgcaa
    20701 ttctttttcc agggaaaggc aaatgagttg gttaaaatat tcagcaaggc caatgcggaa
    20761 cagaaaagca gggccaaaga attacttacc cgtcttgata tcactaatac atcgctgtac
    20821 aataaagagt tgaaatgaga attgtaatcc tatttttttt tatccttgta gcaggcagtg
    20881 ccgggtgtaa aaagaaaagc agtatacccg gaaatgtact ggcacagcaa aagatgcagg
    20941 cagtgctctg ggatatgata cgagcggacc attttattaa taattacatg gtgataaaag
    21001 atagttcgct caataaaagg gtagcgggtt ttaaaatgta taaccgtatt ttaggtattc
    21061 ataaaataag tgaagaggat ttcaaaaaga gtttttcatt ttaccgttcc aatcctgaat
    21121 taatgtggcc cataatggat tctatcagca aaatgtctga tgaagcgcct acccaacaga
    21181 ttaaacctgt accgctggag gattctttac aacaaaccgg accttctact gatacaactt
    21241 caccccggaa aatcaagccg gtatcgctgg actaaccggt aatcttcaaa ccggcggatt
    21301 attgcaagtt gcttaatttt actttctaat atttcatttc atgaataaag tagtagcagg
    21361 agctgatgaa gcaataaaag atattaccga tggagtaaca atcatgttgg gtggtttcgg
    21421 tctttgcggg ataccggaaa actgtattgc tgctttggtg aaaaaagggg tgggtaatct
    21481 tacctgcatt tccaataatg ccggtgtaga tgactttggt attggattaa tgctgaagca
    21541 aaagcaggtg aaaaagatga tttcatctta tgtaggtgaa aatgccgagt ttgaacgaca
    21601 gttattgagt ggtgaattgc aggtggaact tattccgcag ggaacgctgg ctacccgttg
    21661 tttggcaaca gggtatggca tgcccgccat ctttacaccg gcaggagtgg gcacagaagt
    21721 ggcggaggga aaggaggtac ggcaatttaa tggcaaggac tatttgctgg aattggcttt
    21781 tgaagcagat tttgcccttg tgaaagcctg gaaaggggat acgcagggca atcttattta
    21841 taaagagact gcccggaatt tcaatccgct gatggcaatg gctggtaagg taacaattgc
    21901 tgaagtagag atattggttc cggaaggcga acttgatccc gatgcagtac ataccccggg
    21961 tatttatgta caccgcattt tccagggaac tgattatgaa aaacggattg agcagcggac
    22021 agtcaggaaa cgaaattgag tgaccgggag tcttcaactt aaatcacttt taaaccagcc
    22081 tgctggttgg caggtcttca aagcatcatg gcattaacta aagaacagat agcaaaacgt
    22141 atttccaggg aattaaaaga gggatactat gtgaaccttg gaatagggat accaactttg
    22201 gtggctaatt atattccgga ggatatgaat gtggtattgc aaagcgagaa tgggttgtta
    22261 ggaatggggc ctttccctct tgaaggagat gaagatccgg atttaataaa tgcaggtaaa
    22321 caaacaatca ctacggtgcc cgggtcagtt ttttttgatt ctgccatgag ttttggaatg
    22381 atcagggccg gtaaagttga ccttaccgta ctaggtgcaa tggaagtaag cgataaggga
    22441 gatatcgcta actggaagat tcctggtaaa atggtaaaag gcatgggtgg ggcgatggac
    22501 ctggtggcca gtgcaaaaaa tatcattgtg gcgatgatgc ataccaatcc caaaggagaa
    22561 tctaaattat tacccgcttg tacattgcca ttgacaggtg caggatgtgt taagaagata
    22621 gtttcagacc ttgccgtact tgatgtaacg ccggatgggt ttaaactgct ggaaagggcg
    22681 cctggtgtaa gtgtggaaga gataaaagca aagactgcag gcaggcttat cgttgaagga
    22741 gatattccgg agattgaggt gtgaagaagg aggtaggaag taggaggtaa gaagtaggaa
    22801 gaatgaggta gtaggtagga gttttttgtg ccttattgct aaaaaagttt aaatacctta
    22861 ctatgataaa ggcggtggct gatcttgatg tattcaatat ttcatatgcc ctggccatgg
    22921 atatttatca cctggcaaaa aatttcccga aagaagaaaa atattcgctg accgatcaga
    22981 ttatccggtc gtcaaggtca atttcagcaa atattgctga gggttggggt aaaagggtct
    23041 atgaaaacga attcaggaaa tatcttatct actccatggg atcactggaa gaaacaaagg
    23101 tatggctgct tttttcaaga gattgtggtt atgtttctgc agatgtttat acccggatta
    23161 gcatgaaatg tgacgagaca ggggctaaaa tttttaagtt gtatggaaac tggaagacat
    23221 tttagatttc agtctatttg gattcatact tccttcctct tacttcatat ttcagctctc
    23281 actctatcaa attattcttc accgcaaaga aaactaatcc cgctgtattc ttactgccaa
    23341 accgttccat cagcctgtct ttaatggcct caacagttcg ggggctgatt tccatctttt
    23401 gagcaatttc tgcagctgta aattccatgc aaataaagcg gagcacatct ttttctttat
    23461 cactcagggt aatttcactg ttcagtgagg gtaccacttt ttgccgggaa tgagattttt
    23521 tcaacagtat ccggttcaca aaattattca ggtaataacc cttctcatac acttccatga
    23581 tggccctgcg gatctcctgt ggctctgcac tttttaataa ataaccattg gcgccattct
    23641 ccatgaggtg atatacaaaa cgttcatctt cgtacatact caggataagg acctttatct
    23701 ggggatattg tttgctgatt gttttggtag cttctatgcc atccttgccg ggcatacgca
    23761 aatccatcag taccacatcg ggttcagaag cggctaaacc gttgagcaga tcctcaccgt
    23821 tctctgcctc ttgtacaaat tttacattgg aataaggacg caacgaaagg ataactccct
    23881 tgcgaaaaat tttatgatcg tcagctattg ctaccttgat gattcccata gtatcagata
    23941 agggataaaa ttagcaaata atcaggaaca aaccttagcg catctcttat tcgtaaggtt
    24001 catctttggg caactcgatg gtcaccttgt aataagtttg tgaaatatcc ttttcaaaat
    24061 tgatattgcc atctactacc cgcaggcggc tggtaatgtt tttcagaccg agtcctacat
    24121 tacttttatt cagcttttca aaatcagcct gtaccagccc tcttccgtcg tgatggatgc
    24181 gaaggtaaaa cttgccggca tgaacgttct gggtgagatg gataaaactg gagttactgt
    24241 gtttaagaat attattgata agctcctgca ctacccggaa aatgatcagt tccttttctt
    24301 ctttcagcct ttctttatac tcatgaaaac ggctgctggc attgatggca ccggagccgc
    24361 tgattttctg gaacaggtca ttgatggcgg attctaatcc aaaattttta agggtgggtg
    24421 gcatcaggct gtggctgata ttacgcacca gttgtatggt atcatcaatg atctgtcttg
    24481 cctggaagat agattgtaac tgggtagctt tatcctggtt caccaggttc tcattcaggt
    24541 aaagtcgggc cgtggccagc aggggtccgg catcgtcatg caggtcagca gccaggcgtt
    24601 gacgttcttc ttcctgaagt ttgatggaag cgttcagcaa caatctttgt tgggcctgct
    24661 ccatggtttg tatcttttca ttaaagcgtt ttacccttcg ctggtgaaga ataatgaaaa
    24721 tgatcaagcc tacggtaagc aacagcatac ccagggtgcc cagtaaaagc acaaaggaaa
    24781 ctccggaact gttggtggct tgcagtaaga acatataggt aggttagtaa tgggtggaat
    24841 ttatataata tctaagtttg gaatatcaga ttttttgagt ttagaatttg gttgttcatt
    24901 tatatatgaa gggtatccaa gtaataccat tgcaaataat agatttttta agatttcagg
    24961 aatgtatgtt a
//
LOCUS       DAORTI010000042        24936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1555_length_24936_cov_7.338129, whole genome shotgun sequence.
ACCESSION   DAORTI010000042 DAORTI010000000
VERSION     DAORTI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24936
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_24936_cov_7.338129"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..967
                     /locus_tag="PLO70_07415"
     CDS             260..967
                     /locus_tag="PLO70_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74329.1"
                     /translation="MKNLVRCLLTFFISTLAVAQETDGYYLNKTGDTIRGKVQVPLKP
                     KLKLGASSQSDPDPLRDDVPNESKEIDYSKLTFDFKFSEDGNKFKKIDRLKVKGFGFV
                     YNDHHYDFVTWDVAANKQLYLVPATGDVVPDGVYFILRSVEGALPIYSLFQEIEMSKR
                     NSGRKEYDGNATKRDIIFKHPTRGFLYISNQYPMMMKLPDALQYLELEEAFIRTLNKK
                     DNLFEIVKKYNAWKVKN"
     gene            complement(1147..2034)
                     /locus_tag="PLO70_07420"
     CDS             complement(1147..2034)
                     /locus_tag="PLO70_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74330.1"
                     /translation="MKQLFYCLAFLIIAGCSSAQLFEVGARVKLKWSDNWYEGTIVEL
                     SDKQYKVDLDNLGAEYTQWHTKDKLVLLTANTQTKSKPVQGQANQEPTTPVKGLTGKL
                     YLRTSFFGTSLSISTIFLSSNGVIVRDAKNGVDPLDLEKEKQLNAYNTGTYIINGDKM
                     NINWSNGKTASWRMEYKNGVLSGLDGGVVTLQTKMPAGYTLNGRFAAAGVTANASSSQ
                     TYTFKSDGTFSLKSTGNVDNTEETGTATDNKNGQYTITGNTLFLKFNSGQTTRSVICI
                     STIGGKKHLVINRGSYPQE"
     gene            2429..2989
                     /locus_tag="PLO70_07425"
     CDS             2429..2989
                     /locus_tag="PLO70_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ74331.1"
                     /translation="MKLYIKNMVCSRCKMVVKDQLEQLGLHTLAVNLGEVEIEGNLSK
                     DQLLQLDTTLKSVGFELIDNKKSQIIEKIKTAIVTLIHHSDPDLKTNLSEFITSQIHQ
                     DYNYLSNLFSEVEGTTIEKYFITQRIEKVKELIVYNELTLSEIADQLGYSSVAYLSNQ
                     FKKITGFTPSYFKSLKEYKRKNIEEL"
     gene            3067..3780
                     /locus_tag="PLO70_07430"
     CDS             3067..3780
                     /locus_tag="PLO70_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQZ74332.1"
                     /translation="MTHTYKVTGMTCGSCEAKVKSSLLMLPNVTGVEVSKDTQSATIT
                     MDKHIALSTFQNALDKKYSITATEHRETTEQAKSWFSTYKPILLIFAYITAIAIIGAT
                     HQKTFNWMQAMTIFMAGFFLTFSFFKMLDLNGFAESYSMYDIVAKKLKAWGFMYAFIE
                     LALGIAYATNFQPFITNIVTFLVMTISIIGVLQSVLKKRKIQCACLGAVFNLPMSTVT
                     IIEDALMIVMSAAMLLVNI"
     gene            3792..4310
                     /locus_tag="PLO70_07435"
     CDS             3792..4310
                     /locus_tag="PLO70_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HQZ74333.1"
                     /translation="MNHQIHTKQGHLHHATHQTHTDEEHQLFLKAEKVSDFKLAVSAT
                     LHCLVGCGIGEVLGMILGAYFGWGMIDTMIVAITLGFVFGFLLGMIPLLRKKFSIKDA
                     FKIVFAAEFISIVVMEAFEVGTQLIIPGVMEAGLSDSIFWIGMLAALAVGFIAAFPVN
                     YYMIRRGVRHQH"
     gene            4328..4717
                     /locus_tag="PLO70_07440"
     CDS             4328..4717
                     /locus_tag="PLO70_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74334.1"
                     /translation="MKKIAIIILFLIYGSVNMGAVIHLHFCTDKFVEWSLWYSGKDKK
                     CSNCGMKEKKADCCKDDHKQVKLNTVHPKSATAQNIQFIDVPVLATPIAGFSAKGITK
                     SKTFPATNDPPKNPGERLYFLYSAYLI"
     gene            4802..5674
                     /locus_tag="PLO70_07445"
     CDS             4802..5674
                     /locus_tag="PLO70_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HQZ74335.1"
                     /translation="MKTILLIAAVVLLSTTACQAQINNARRDTIKVYGNCGICETAIE
                     KAADKKNIAKADWNKDSKMATITYDSKKTDLDAVLKNIALAGYDNQNFLAPDAAYNKL
                     PGCCKYERVKKQMAMVIQPSTDTTIPKPGPVSHQHDGITNAAQETNLLKAVFDSYFSL
                     KDELVKTDGNKASAKAKELKTAINAVKMEKLPMDVHTIWMKVVTGLKDDAENINGTND
                     IARQRDHFMSLSTNMYELIKIAKPVETIYYQFCPMANNGKGANWLSKESNIKNPYYGS
                     QMLTCGKTVETIKQ"
     gene            5739..8030
                     /locus_tag="PLO70_07450"
     CDS             5739..8030
                     /locus_tag="PLO70_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQZ74336.1"
                     /translation="MAIQKTVCKKMLPLAAFFTLLVSNLFSQKIVRYDLYVKDTLVNF
                     TGKEKRAIAVNGQIPMPTLTFTEGDTAEIHVHNELNESTSLHWHGLFLPNKEDGVPNL
                     TQMPIKPHTTHIYRFPIIQHGTHWYHSHSGLQEQIGMYGNFIMLKKQTDPAFRKGIDD
                     LPTVPIVLSEWTDYRPENVHRLLHNATDWFAIRKNATQSYGEAIKAGHFNTKLKNEWK
                     RMLAMDVSDVYYDKILMNGSPATDIKSIDGKILKAGDKVRIRISNGGASSYFWLTYAG
                     GKITVVANDGNDTAPVEVDRLIIAVSETYDVVVTIPADSTAYELLATTEDRTNSASCY
                     IGNGIKQLLSPLPRLKYFEGMKMMNDMMKMNGDLDDMGMKMSLNQMDMNVVMYPEITG
                     ELKKKKDIKMRDPEPGNDHSNHAGNKYDANALSEIVTLNYAMLKSPHKTNLPKDASVK
                     ELKFELTGNMNRYVWSLNNKVVSEADKILIKKGEIVRLVLYNGSMMRHPMHLHGHDFR
                     LLNGQGENAPLKNVVDIMPMETDTLEFHANVEGDWFFHCHILYHMMSGMGRVFTYENQ
                     APNPLIPNPKLAQRKLFADDRKMHFMFQNDLATNGNDGEMMFQNTRWSIGSEWRLGYH
                     DMHGYETETHIGRYLGKMQWLMPFVGFDWRYRKMGIDEQEKNLFGQTNTKDNRAVLSV
                     GVNYTLPMLVRFQAEIFTDGIFRLQLMREDIPVAKRLRAAFMVNTDKEYMAGLNYILN
                     RSFSVRTHYDSDMGFGAGLTFAY"
     gene            8383..8901
                     /locus_tag="PLO70_07455"
     CDS             8383..8901
                     /locus_tag="PLO70_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HQZ74337.1"
                     /translation="MEYILKDKNLELKYEPGKGSWTYHLQIPNTKNIKGKWGDIKVSG
                     YIDDYKIESKNLAPIKGKDKLLAVNGTIRKAINKSGGDKVTVTLYLLTNKEQVNKKQI
                     LETFKESGVLTAFRRLSKEEQKEIIEDILVQIPEEKQIQKIVSYLEQLSKYVDTNNQK
                     ASLINPERNSIQ"
     gene            9260..9514
                     /locus_tag="PLO70_07460"
     CDS             9260..9514
                     /locus_tag="PLO70_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74338.1"
                     /translation="MTNKDLSEFTDEELLAEAKKMKSFSITNALFIGFLVGVVFYSIV
                     KNSWGMLTLIPLYFIYKMVNDPKNKRTKDLEELLKERKLN"
     gene            9743..10288
                     /locus_tag="PLO70_07465"
     CDS             9743..10288
                     /locus_tag="PLO70_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74339.1"
                     /translation="MTNNNDIEIPKLNFNEEGELIITASPTSSNHDFDFYEGKWKLHN
                     KKLKDRLNNCTEWTTFESTQEMYRVLNGIGNIDNYLATFDGVPFEGMSVRLFNPATRL
                     WSIYWADSAEGILQPPVVGSFENNRGHFFTKDIFKEKKIVVAFRWDVSDKENPVWSQA
                     FSADNGKTWEWNWYMYFSRIR"
     gene            10436..11542
                     /gene="mnmA"
                     /locus_tag="PLO70_07470"
     CDS             10436..11542
                     /gene="mnmA"
                     /locus_tag="PLO70_07470"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQZ74340.1"
                     /translation="MSKKGKVLVAMSGGIDSTVTALMLNAEGYEVVGITMKTWDYMAS
                     GGSKKETGCCNLDSFNDARQAAVEHGFAHYILDIREEFGDFVIENFVEEYLAGRTPNP
                     CVLCNTHIKWRALLKRADALGCEFIATGHYAKIRQHDNGRFVVSKAIDDTKDQSYVLW
                     GLQQDLLSRTLLPLGPYRKTEIRQMAHDFGYPELAKKNESYEICFVPDNDYRGFLKRR
                     VDGLEERVAGGNFIDKNGKILGQHKGYPFYTIGQRKGLDIAMGRPVFVTKIDPDNNTV
                     MLGDEEDLEQNEMIVSKLNLIKYDSITPGMEAVTKIRYKDKGSLSTIYPEGGVMKVRF
                     YENAKGIAPGQSAVFYEGDDVIGGGIIQRGMLNA"
     gene            11609..11965
                     /locus_tag="PLO70_07475"
     CDS             11609..11965
                     /locus_tag="PLO70_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74341.1"
                     /translation="MRDYKKYDVWIKAHELVLFVYKEVIPGFPKSEQFELVSQIKRAA
                     YSIPMNIAEGCGRNSDKDFVHFLDIALGSAHELEYCGLLGADLGFIKKANVDPLNTRV
                     NEVKAKLINLIKAIRK"
     gene            12136..12972
                     /locus_tag="PLO70_07480"
     CDS             12136..12972
                     /locus_tag="PLO70_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74342.1"
                     /translation="MFPRFLFVDLFVNFVSVNKEKPLMRIFSPVRLSLLAILSAALFS
                     CNEKEDFTTEALADYIPLTAKKYITYRLDSMVFTNFGRNTEIRKYQVKHVIDTLITDN
                     LGRPSYRVYRYLSDSTGTQPWQPSGTYFITPLSDQVELIEDNLRFIKMHLPIRNGFSW
                     KGNRFLPSDPYGTIYNFSNDDSMEDWDFYFDGEPSSFSHRGINYTDVHSIEQVDEAFN
                     VPITSPTAYAAKSRSVEKYAKNIGLVYREYELWEYQPNPGGAGGPYKTGFGITMWMVD
                     HN"
     gene            13021..15021
                     /locus_tag="PLO70_07485"
     CDS             13021..15021
                     /locus_tag="PLO70_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQZ74343.1"
                     /translation="MKKILLLFIVAVTFIVESQAQFTRYIVKLKDKGNNTFSLANPSG
                     YLSQRSIERRTRYGIAIDSTDLPVTPSYVTQIRNVPNVTILNVSKWLNAVSIQTSDAN
                     AITAITALPFVKSVGGLAARSTGDEPGPTRNKFVTEEAINPIPELAQRTEQLTGDFFN
                     YGTGSFNEIHLHNGEFLHNIGLRGQGMQIAMLDGGFFNYNSLRAFDSANTNSQVLSTW
                     DFVSRNATVTDDHPHGMQCFSTIAANIPGQFVGKAPKAGFHLFRTEDVASEYPIEEHN
                     WVCGAERADSAGSDIISSSLGYYDFDNAVFNYTYSNMDGNTTMAVIGADLAAKKGLMV
                     FNSAGNEGGNAWRFLVTPSDGDSVVAVGAVNSSGIVGSFSSYGPSGDGQIKPDMASVG
                     VAALVQTSSNTIGTSNGTSFACPNMAGLATCLWQGFPEYNNMKIIQALKQASNKFSTP
                     DDRMGYGIPNLKLAFTNLLTEYATATASANACTVSVTWNSKDVATMKYEIERKVPGES
                     VYTKVGDINAQAGTILTNKSYSFNNTLVGVNPGTVSYRIRQVVDTAAATFTAVYIDTA
                     DVTITAGCFATGTSNQLVMVQPNPVSGSTVTLVVETPYAIPTMSVMMYDAKGSLVMQT
                     KDAKGTGKKLIDLSVSKLSTGKYYISVYDNQKLLGTAELIRL"
     gene            complement(15016..16182)
                     /locus_tag="PLO70_07490"
     CDS             complement(15016..16182)
                     /locus_tag="PLO70_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HQZ74344.1"
                     /translation="MSRYYSYLNSAIQLLGKYRGDEPFSLFLKKHFSTNKKYGSKDRK
                     SISHLCYCYFRLGKAVAEMPVEERIIAGLFLCSLTSNELLQHLRPDLDPHAALPLSKK
                     ILLLDSSFSVNDIFPWGNEFSEGIDYEKFCAAFFIQPDLFLRLRPGNQERVMAKLRQQ
                     EIVFETINESCIAVANSLKVDTIIELNKEAVIQDYSSQQVGKLLAQVKKENVFRVWDC
                     CAASGGKSIMAKDILEDIDLTVSDIRESILYNLKQRFAEAGIKQYKSFVADLTASGSQ
                     PAKQQYDLIIADVPCSGSGTWSRTPEQLYFFDAQKIDEYSLLQKKITSAIIPSLRPGG
                     FLLYITCSVFKKENEFVVEYIKEKYGLAEVEKKSMTGYDKRADTLFASLLQKPL"
     gene            complement(16238..17080)
                     /locus_tag="PLO70_07495"
     CDS             complement(16238..17080)
                     /locus_tag="PLO70_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74345.1"
                     /translation="MKRYLALVIILLSLVYMSCQKEIDWGLGGTGSNNLLSRINSKTG
                     TDSSEVSYFYNTQNRLIREKTTGISGGLTLDNELIINRNAAGIILTTVQKAAALATAG
                     IDSVVTRYNYNTSTSRYTSSVFSLTAFGFSVTDSAVYSYDASGKIISDVHYLATGFLP
                     PIQSLKNDYTYSADGVNITRQDQMAATTPGGPLLPVSSQTYTYDTKTNPLILSHEAVL
                     LSRTSLFNANNSLKTVFTNTADPTANFNLDIIYRYNAAGKPDSAFSIRTPPGELTVST
                     YHYQ"
     gene            complement(17103..18332)
                     /gene="odhB"
                     /locus_tag="PLO70_07500"
     CDS             complement(17103..18332)
                     /gene="odhB"
                     /locus_tag="PLO70_07500"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQZ74346.1"
                     /translation="MIEIKVPTVGESISEVTLLKWNKKDGDYVERDEVIAELESEKAT
                     FEVNAEQAGVLKLGSVNEGDTIKIGDLLASIDETAPKPASAQQAETASAQKTEAAKPA
                     TTTAPVSSVPNDIKASPVASAIIADKKVDPKSVTASGYQGKIMKDDVLAALSNPGKKA
                     FNGAELFSRNVRVEKMSNLRKTISRRLVEAKNTTAMLTTFNEVDMGSIMEIRTKYKDK
                     FKEIHGVGLGFMSFFAKACAVALAEWPSVNAYIDGDQLVYHDYADISIAVSTPRGLTV
                     PVMRNVESMSMADIEKMVVELAGKARDSKLTTEELTGGTFTITNGGVFGSLMSTPIIN
                     IPQSAILGMHKIQERPMAINGQVVIRPMMYLALSYDHRIVDGRESVSFLVRVKELLEN
                     PEQLLIGKDPVKTLLEL"
     gene            complement(18352..21111)
                     /locus_tag="PLO70_07505"
     CDS             complement(18352..21111)
                     /locus_tag="PLO70_07505"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQZ74347.1"
                     /translation="MKDFSYITNSHPEFIENLYRDFVNDPTSVDPEFKKFFEGFDFAI
                     GNSSMTGVGNGKEMPVATGKVISAEVDWMREIKAYRLIVGYRNKGHLIAKTNPIRQRK
                     DRGANIDLAFYGFNEADLDKEFHAGQLIGLGTNSLRNILAHLEKCYASSVGAEFKYIS
                     DQKKIDWLTNEIEKRMLEPLPLDKKRRALEKLNQGVMFEKFLHTKYVGQKRFSLEGGE
                     TTIAALDAIINTAADNDVHEVVIGMAHRGRLNILANIMGKTYEQIFSEFEGTAKMDQT
                     MGSGDVKYHMGYGSEVKTMNDKTIHLKLMPNPSHLEAVNPVVVGFSRAKADVMYHSEF
                     DRILPILIHGDASVAGQGVVYEVLQMSYLKGYYTGGTIHFVINNQIGFTTDFDDARSS
                     DYCTSLAAAIQAPVFHVNGDDPEAVIKCAEIATRFRQEFNSDFFIDMVCYRRHGHNEG
                     DDPKFTQPHLYALIDKHPNPREVYIKYLLENGEPDAQQLAKEMEKKFWADLQERLDEV
                     KQNPLPYHYQPPELEWKKLRRATPEDFDSSPVTSITEADFRKVFDAMMIWPADFKPLR
                     KVEKILQDKIKLFETERKVDWATGELLAYGSLLLEGKDVRMSGQDVRRGTFSHRHAVL
                     RDENTDKPYSRLSHIPGATGKFRIYNSLLSEYGVLGFEYGYALANPNALVLWEAQFGD
                     FANGAQTMIDQFIAAGEQKWNRMNGVTMLLPHGYEGQGPEHSSARLERFLQLCAELNM
                     VVTNITTAANFFHALRRQLAWPFRKPLINFSPKANLRHAGSYSRIEEFTSGGFKEVID
                     DAQADAAKVKKVLFCCGKIYFDMAERQQKENRNDIAIIRLEQIYPLPYKQLDALQRKY
                     SKATWFWVQEEPMNMGAASFLKMNLTGINYGVIGRQPSASTATGYNKVHLQEQAEIIE
                     TAFSI"
     gene            21377..21628
                     /gene="rpsT"
                     /locus_tag="PLO70_07510"
     CDS             21377..21628
                     /gene="rpsT"
                     /locus_tag="PLO70_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQZ74348.1"
                     /translation="MANHSATKKDARQAAKRRDRNKYYGKTTRNAIRDLKAAPVKEMG
                     EKFPLVESMIDKLAKRGVIHKNKAANLKSKLAKKVNSAK"
     gene            21732..23459
                     /locus_tag="PLO70_07515"
     CDS             21732..23459
                     /locus_tag="PLO70_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQZ74349.1"
                     /translation="MRHFFLIFFLLVSFIAGAQKLTTRFEQSNSTQTPDYFEIIDWWK
                     KLDLQSGKIKMLTMGITDAGYPLHLVVVSNNGDYNFSNIRKNNKRIILINNGIHPGEP
                     DGIDASMLLARDIVAGKYKIPDNIVLAIIPVYNIGGCLNRSANYRVDQNGPEEFGFRG
                     NSQNLDLNRDFIKCDSRDARAFTEIFHLTDPDVFVDNHVSNGADYQHVMTLLTTQNSK
                     LGGTMGEYISKEFEPALYSLMKTKGFDLIPYVNSFGDTPENGWPEYWDSPRYASGYAA
                     LWHTFSFVPESHMLKPYDQRVKATYALMQSFIEFTKKNSEQIKSIRNQTKKSVETANE
                     FPVSWSLNRSQFKEVMYKGYEAGRKPSEISGLPRLFYDRNKPFEKKVKIYNNFAPKTI
                     IKKPKAYVIPQGWWKVIELLKLNKVQMSQLKKDTIIEAEVYKIEDYKTTPRQYEMHHL
                     NSDVKTTTTMQTIRFLKGDWYIPMNQPANRFLMETLEPQAEDSYFAWNYFDAILGQKE
                     GYSAYVFEDIAAGYLKNNTELKVKLEQRKQSDTAFAGNARAQLNFVYQNSSWYEPAHM
                     RYPVYRILK"
     gene            complement(23460..24701)
                     /locus_tag="PLO70_07520"
     CDS             complement(23460..24701)
                     /locus_tag="PLO70_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ74350.1"
                     /translation="MNQKERIILFLLAGLNFTHILDFMIMMPLGNYLMPYFHISPWQF
                     SLLVGAYSISAAVSGFTAAFIVDRFDRKKVLLVSYAGFIIGTLACGIAPTYGLLLGSR
                     ILAGLFGGIIGAQVLSIIADLFVYERRGTAMGAVMSAFAVASTLGVPFSLYLTNLFNF
                     NWHVPFLLVGGIGIVLIPLIYKFIPGMSAHLKSVGNRESTWRVLGKVFESPAQQQALL
                     FSGLLMMGHFLIIPFLNPYLEFNKGFSKDLTPMVYLVGGISSFAAAIFLGRFSDKKGK
                     LPVFSISVFLSLFMVLVITNMPDVHFSIVLAFFAVWFILATGRAVTAQAMVSEVVKTE
                     QRGSFMSFNGSMQQLGTFIASMAAGVIVLKDKTGKIQRFDWLGYLSIAVLLISLVLGR
                     ILFKKIDRADKTLSDKNFINE"
     gene            complement(24816..24927)
                     /gene="rrf"
                     /locus_tag="PLO70_07525"
     rRNA            complement(24816..24927)
                     /gene="rrf"
                     /locus_tag="PLO70_07525"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         7900 a         5133 c         4943 g         6960 t
ORIGIN      
        1 tttgaataag cagggccggg caaagtaaca tccgcttgca ggcatcatct gcggttccgg
       61 cccgatgtac aattctcagc tttagttcaa gacttcaaca ccatatttgt tccgtattga
      121 aataagctga ttagcatcaa cccttttacc gggctttgta acaggccctg cgttcaataa
      181 attccctgcc cctgcaaata ccggtccgat gcaatgcggt gagctttgtg catcactata
      241 aaatttctta atttcaatta tgaaaaattt agtacgctgc ctgttgacat tttttataag
      301 tacccttgcc gtcgcccagg aaacagatgg ttattacctt aacaaaacgg gtgataccat
      361 tcgtggtaaa gtacaggttc ccctgaaacc taaactgaaa ttaggggctt cctcgcaaag
      421 cgacccggat ccacttagag atgatgtgcc caacgaatca aaagaaattg actacagtaa
      481 attgactttt gactttaagt tttcggaaga cggcaataag tttaaaaaga tagaccggct
      541 gaaagtaaag ggatttggct ttgtgtataa tgatcatcac tatgactttg taacatggga
      601 tgttgcggcc aacaaacaac tttacctggt accggccacc ggtgatgtag tgccggacgg
      661 cgtttatttt attcttcgca gtgtggaagg agcactgccc atctactcgt tattccagga
      721 aattgaaatg tcgaaaagga attccgggcg caaagaatat gatggaaatg ctaccaagag
      781 agacattatt ttcaaacatc ccaccagggg attcctctac atctccaatc aatacccgat
      841 gatgatgaaa ctgccagatg ccctacaata cctggaattg gaagaagcat ttatcaggac
      901 actgaataaa aaagataatt tatttgagat tgtaaagaaa tacaatgcat ggaaagtaaa
      961 gaattgattg agctattttt aaaattctac gtaacagttt ctttgcagga aaggaacatt
     1021 tagtaatgat ctgaaaagaa taaattgttt cgcaaaaagc gcttctcctg ttcgaagcgg
     1081 accggtaaac tctctttcaa aagttttcac tgacaccttt tcgtaagtgt caattgcatt
     1141 tgtgaattat tcctgcggat atgaaccccg gttgatgacc aggtgttttt tgccaccgat
     1201 agtggaaata catataacgg aacgggttgt ctgaccacta ttgaatttca gaaacagcgt
     1261 attccctgta atcgtatact gaccattctt attatcggtg gctgtgcctg tttcttctgt
     1321 attatccacg tttccagtcg attttaaact gaaagtccca tcgcttttaa aagtataggt
     1381 ctggctgctg ctggcattcg ctgtaacacc ggctgcggca aaccgtccat tcagtgtata
     1441 cccggcaggc attttagttt gcagggttac tacaccacca tcaagtccac tcaaaacccc
     1501 gttcttatac tccatacgcc agctggccgt tttcccatta ctccagttaa tattcatctt
     1561 atccccgtta atgatatagg tgccggtatt atatgcgttg agttgttttt ctttttcaag
     1621 atccaacgga tccaccccgt ttttggcatc ccttacaata acaccattac tgcttaaaaa
     1681 aatggtagag atacttaaac tggtaccgaa aaaagaagtg cggaggtaaa gtttcccggt
     1741 aagtcctttt acaggtgttg tgggctcctg gttggcttga ccctgcaccg gtttgctttt
     1801 tgtctgcgta tttgctgtca gcaaaaccaa tttgtctttt gtgtgccatt gtgtatactc
     1861 ggctcccagg ttatcaaggt ctactttgta ttgcttgtcg ctcaattcaa cgattgttcc
     1921 ttcgtaccag ttatcactcc acttcaactt tacccttgct ccaacttcga ataattgtgc
     1981 ggatgagcac ccggcgataa taagaaatgc caaacaataa aaaagttgct tcataaatcc
     2041 tgagattttt atgaaagtga atgtatataa gaattttgta aatgataaga atattttacc
     2101 aatccaccca gcggaaaaac gaatgatgta ccattaccaa attggagaac ggtacttata
     2161 ttataagact tgcaataaaa ggaaaaaatc tgacccgttt caatgtgatg cctggtaaat
     2221 atatccggtt acaaccactt gttgaaaatg ggtactttta aagatcaacc cgaccattaa
     2281 caggattcaa cccgaagctt tagccggatt atttatggaa gccttaaaaa ctgcagacta
     2341 aaacttgagc ttgtaagcag acttaggact gaccaattat agtaaccttt caataaattt
     2401 gtaaattacc acacgatggt cagacgatat gaagctttac attaaaaata tggtttgcag
     2461 ccgatgtaaa atggtagtaa aagaccaact ggaacagtta ggccttcaca ccctagctgt
     2521 taatttgggc gaagtagaaa ttgaaggcaa cttatcaaaa gatcaattac ttcaacttga
     2581 cacgacatta aagtcagtag gctttgaact catagacaat aaaaaaagcc agatcatcga
     2641 aaaaataaaa accgctatcg taacactgat ccatcattca gaccccgact taaagacaaa
     2701 cctttctgag tttatcactt cacaaataca ccaggactat aattatttaa gtaacctctt
     2761 ttcagaagtg gaaggcacaa ctattgaaaa atactttata acccaacgaa tcgaaaaagt
     2821 taaagaactg atcgtatata acgaactaac tttatctgaa attgctgacc aattaggtta
     2881 tagcagcgtg gcgtatttat ccaaccaatt caaaaaaata acaggcttta caccgagtta
     2941 cttcaaatcg ctgaaagaat acaaacgtaa aaatatagag gaactgtaaa tcttacaact
     3001 cttatacaga attccacaac aaccagtccg ggtacctaaa ttacttttgt gtcaaacaaa
     3061 tgaattatga cacataccta taaagttacc ggcatgacat gcggcagttg cgaggcaaaa
     3121 gtaaaaagca gtttgctgat gttaccaaat gttaccggag tggaagtttc gaaagatacc
     3181 cagtcggcca ctattacaat ggacaagcat attgcacttt ctacttttca aaatgcattg
     3241 gataagaaat attctatcac cgcaacagaa caccgggaaa ccacagagca ggccaaaagc
     3301 tggttttcaa catacaaacc gatcttgctg atttttgcat acataactgc aattgcaatt
     3361 ataggagcaa cacaccaaaa aacatttaac tggatgcagg ccatgactat ttttatggct
     3421 gggttctttc tcaccttttc tttctttaaa atgctggacc tgaatggttt tgccgaaagc
     3481 tacagcatgt atgatattgt ggcgaaaaaa ctaaaggcat ggggttttat gtatgccttt
     3541 atcgaattgg ccttaggcat agcctatgct accaacttcc aaccctttat taccaatatt
     3601 gtcacctttt tggtcatgac aatcagcatc atcggggtat tgcagagtgt attgaagaaa
     3661 agaaaaatac aatgtgcctg tttgggtgca gttttcaacc tgcctatgag taccgtaact
     3721 attattgaag atgctttaat gatagtaatg agtgcagcaa tgctattagt aaacatttaa
     3781 actatataat tatgaaccac cagattcaca caaagcaggg tcacctgcat catgcaactc
     3841 atcaaactca tacggatgag gaacatcaac tttttttaaa agctgaaaag gtgtctgatt
     3901 ttaaactggc ggtaagtgca acactccatt gcttagtggg atgcggcatt ggtgaagtac
     3961 tgggtatgat tcttggagct tatttcggct ggggcatgat tgacacaatg attgtagcca
     4021 tcacgctggg ctttgtattt ggctttttac tgggtatgat accactactc cgtaaaaaat
     4081 ttagtattaa ggatgcattt aaaattgtgt ttgctgctga atttatcagc atagtagtga
     4141 tggaagcatt cgaggtcggt actcagctta ttatacctgg cgtaatggaa gccggcctgt
     4201 cagattcaat attttggata gggatgcttg cagctcttgc tgttggcttt attgcagcat
     4261 tcccggtcaa ttattatatg atcagacggg gagtcaggca tcagcattaa aagtataact
     4321 ttagtaagtg aagaaaatag caatcataat actttttctt atctatggct ctgtaaacat
     4381 gggtgctgtt attcatttgc atttctgtac ggacaaattt gtagaatgga gtttgtggta
     4441 cagcggcaaa gacaagaaat gcagcaactg cggaatgaag gaaaagaaag ccgactgctg
     4501 taaagacgat cacaaacagg taaaactaaa taccgtacat ccaaaaagtg caacagcaca
     4561 aaacattcaa tttatagatg ttcctgtact agctactcca attgccggct ttagtgctaa
     4621 aggaattact aaatcaaaga cttttccggc tactaacgat ccgccaaaga accccggaga
     4681 acggctttac tttctctata gcgcatattt aatttaattt ataaaagttc tccaatgccg
     4741 gcgacaggaa aagcacaccg gctaattgtg gatttttaaa tcaataaata aaaacaaaaa
     4801 aatgaaaaca atattattaa tagcagctgt tgttctatta tcaactactg cttgccaggc
     4861 tcaaataaat aatgccagaa gagacactat taaagtgtat ggcaattgcg gaatatgcga
     4921 aaccgctatt gaaaaggccg ctgacaaaaa aaatatagca aaagcagatt ggaacaaaga
     4981 ctccaaaatg gcaactataa cctatgacag taaaaaaacc gatctggatg cggtattaaa
     5041 aaacatagcg cttgcaggtt atgacaatca aaattttctt gcacctgatg ccgcttataa
     5101 caaattgccc ggctgctgta aatatgaaag ggtgaaaaag caaatggcaa tggtaataca
     5161 accctcaaca gacaccacaa tccctaagcc agggcctgtt agccaccaac atgatggcat
     5221 aaccaatgca gcacaggaaa caaacctgtt gaaagctgta tttgacagtt atttttcttt
     5281 gaaagacgaa ttagtgaaaa cagatggaaa taaagcatcc gcaaaagcaa aagagttgaa
     5341 aacagctatc aatgccgtga aaatggaaaa gttgccaatg gatgtgcata caatttggat
     5401 gaaagtagta accggcctga aagatgacgc cgaaaatata aacggcacca atgacatagc
     5461 ccggcagcga gaccatttta tgagtttatc aacaaatatg tatgagttaa taaagatcgc
     5521 aaaacctgtg gaaacaattt attatcaatt ttgtccgatg gcaaataatg gaaaaggggc
     5581 caattggtta agtaaagaaa gcaatattaa gaatccttac tatggttcac agatgcttac
     5641 ttgcggaaaa acagtggaaa caataaagca atgagacaaa gaacctgttt aaaataaaga
     5701 aaccgcctta tcaattttac ataaatgaat tttctaaaat ggctatacag aaaactgttt
     5761 gtaaaaaaat gttgccgtta gcagcctttt ttacgctact tgtatcaaat ctattttctc
     5821 aaaaaattgt acgatatgat ttgtacgtaa aggacaccct cgtcaatttt acaggcaaag
     5881 aaaaaagagc cattgcagta aatggtcaaa taccaatgcc cacacttact ttcacagaag
     5941 gcgatacagc ggaaattcat gtacacaatg agttaaatga atcaacttct ttgcattggc
     6001 atggtttgtt tttacccaac aaagaagatg gtgtcccgaa tcttacacaa atgcccatca
     6061 agccacatac aacacatatt tacaggtttc cgattataca gcatggcaca cattggtatc
     6121 acagtcacag cggattgcag gagcaaattg gaatgtacgg aaactttata atgcttaaaa
     6181 aacaaaccga cccggccttt agaaaaggaa ttgatgattt gccaaccgtt ccaatcgttt
     6241 taagtgaatg gaccgactac agaccagaaa atgtacaccg cctgttgcac aatgcaacag
     6301 attggtttgc tatcagaaaa aatgcaactc agagctatgg cgaagctata aaagcagggc
     6361 atttcaacac caaactaaaa aacgaatgga aacgaatgct ggccatggat gtaagcgatg
     6421 tgtattacga taaaattttg atgaacggaa gtccggctac tgatataaaa agcattgacg
     6481 gaaaaatatt gaaagccggc gataaagtac ggattcgcat atcaaatggt ggtgcatcct
     6541 cttatttttg gcttacgtat gcaggtggta aaataaccgt tgtggcaaac gatggcaacg
     6601 atacagcgcc tgtagaagta gacagattaa tcattgccgt ctcagaaact tacgatgtgg
     6661 tagttaccat tcctgccgat agtactgctt acgaattgct ggcaactacc gaagaccgga
     6721 ccaattcagc atcctgttat attggaaacg ggatcaaaca acttctgtcg cccctgccaa
     6781 ggttaaaata tttcgaagga atgaagatga tgaatgacat gatgaaaatg aatggcgacc
     6841 tggatgatat gggaatgaaa atgagtctga atcaaatgga tatgaatgta gtgatgtatc
     6901 ctgaaattac tggcgaactt aaaaaaaaga aggacataaa aatgcgggac ccggaaccag
     6961 gaaatgacca ttccaatcat gccggtaata aatacgacgc taatgcactt tctgaaattg
     7021 ttacgctaaa ctatgccatg ctcaaatcgc cgcataaaac aaatcttcca aaagatgcat
     7081 ccgtaaagga actgaaattt gaacttaccg gaaacatgaa tcgttatgta tggagcttga
     7141 ataacaaagt agtttccgaa gcggataaaa tacttatcaa aaaaggtgaa atcgtcaggc
     7201 ttgttttgta caacggttca atgatgcgcc accccatgca cctgcacggt catgactttc
     7261 gtttactgaa cggacaagga gagaatgctc cgctaaaaaa tgtggtggac attatgccaa
     7321 tggaaactga cacattggaa tttcatgcaa atgtggaagg tgattggttt tttcattgcc
     7381 atattttata tcatatgatg agtggaatgg gcagagtgtt tacatacgaa aatcaagctc
     7441 ccaatccatt aataccaaat cccaaactgg cacaacgcaa actatttgca gacgacagaa
     7501 aaatgcactt catgtttcag aatgatcttg ccacgaatgg gaatgacgga gaaatgatgt
     7561 ttcaaaatac ccgttggagc attggcagcg aatggcgttt aggctatcat gatatgcacg
     7621 gatacgagac ggaaacacat attggcaggt atttggggaa aatgcaatgg ctcatgccat
     7681 ttgtcgggtt tgactggcgt tacagaaaaa tgggtattga tgaacaagaa aaaaatctct
     7741 tcggacagac aaacacaaaa gacaacaggg cggtattaag tgttggcgta aattatacat
     7801 tgcccatgct ggtccgtttc caggctgaaa tttttacgga tggtattttt cgtttacaat
     7861 taatgaggga agacattccg gtagcaaaaa ggcttcgtgc tgcttttatg gttaacaccg
     7921 acaaagaata tatggcgggg ctcaactata ttttaaaccg cagcttctca gtgcggacac
     7981 actatgacag cgacatgggc tttggtgcag ggttaacatt cgcctattag acaatgtagg
     8041 aaagcaacct atgtattggt ttgtgtcccc acgaactatt ttttaacttt actggaatac
     8101 gtttagtaag gaaaggaagc ggtgcgatta aatacttact ggttctcatt tagttacggc
     8161 agattaaaaa aatattgtgc aaattgaatt actattttta actataatag ctgcagtagc
     8221 tataagtatt tcatgcagaa agggagccag tataaagcct ttatttataa atgaattggg
     8281 caaagaccat attcccatca tcaggcactt gtttgatagc aacccctact ttcaggttgt
     8341 tgacaccgaa caatggctgg gtaaatcaac aatgaaaacg aaatggagta cattttaaag
     8401 gataaaaacc tggaattgaa atacgagccg ggtaaaggtt cctggaccta tcatttgcaa
     8461 atacccaata ccaaaaatat aaaaggcaaa tggggtgaca ttaaagtctc cggatatatt
     8521 gatgactaca aaattgaatc gaagaacctt gccccaataa aaggaaagga taaactttta
     8581 gcagtaaacg gtaccatcag gaaagccatc aacaaaagcg gtggagataa agttaccgtt
     8641 acactttacc tgctgacaaa caaagaacag gtaaacaaaa aacagatcct ggaaacgttt
     8701 aaagagtcgg gtgttttaac agcattccgg cgactatcga aagaggagca aaaagaaatt
     8761 attgaagata ttttagttca aattcctgaa gaaaaacaaa ttcaaaaaat tgtcagctac
     8821 ctggaacaat taagtaaata cgttgataca aacaatcaaa aagcttcctt aattaacccg
     8881 gaacgtaatt ccatccagtg aaacaaatag gttgctgtag ctacccatta gttgcacatt
     8941 cccgttggct ttcacatcca gccgggcaaa accgaggcct gatacaaccg tgaagatgaa
     9001 aggccagcag gcaacaggcg gttttgcact actggggcag acggaagcag tgtttcagca
     9061 actataacat ttctcagcag ttgttcgggc aggacaagca actttcgact aattgcaaat
     9121 catcaacttt taattctatt ttcatcatca gttggcctgg ctggacggaa tcctatttcc
     9181 ccagcagcac aaagctttcg gccgttagct gcttttatga tgacgactta acaaaaaata
     9241 atatttaacg aaataaataa tgacaaacaa agacctttct gaattcacgg acgaagaatt
     9301 attagccgaa gcaaaaaaaa tgaaatcatt ttccattacc aatgctttgt ttattggatt
     9361 tttagttggt gttgtatttt acagtattgt taaaaacagt tggggaatgc ttacattaat
     9421 ccctttatat tttatctaca aaatggttaa tgacccgaaa aataaaagaa ctaaagacct
     9481 ggaagaatta ttaaaagaac gaaagttaaa ctaagccata attgaagatg aaaacttatt
     9541 gttctgaaat gttcgacagt ttgcagtcga aatatgtttc gtcctgaaat aggttgacta
     9601 taaaacaacc attttatgga cttaaaagaa cagattgtta aggaatgcct ggagctaggc
     9661 tgacagttcg caatgaccat gccatcgaaa atgcttcaac ctaaccaaca tccggcaaac
     9721 acgaaattga taaaaaataa tcatgacaaa taacaacgat atagaaattc caaaactaaa
     9781 ttttaacgaa gagggagagt tgatcataac agcatctccc acttcatcta accacgactt
     9841 tgacttttat gaaggcaaat ggaaactgca taataaaaaa ctaaaagacc ggttaaacaa
     9901 ctgtaccgaa tggacaacat ttgaatcaac ccaggaaatg taccgggtgt taaatggaat
     9961 tggcaatatt gacaattacc tggccacatt tgacggtgtg ccctttgagg gcatgtcggt
    10021 aagacttttc aacccggcaa caagattatg gagcatttac tgggctgact ctgctgaagg
    10081 gatattacaa ccaccggtgg taggttcatt tgaaaataat aggggccatt ttttcacaaa
    10141 agatattttt aaggaaaaga aaatagtagt tgcatttcgc tgggacgtta gcgacaaaga
    10201 aaacccggtt tggagccagg cattttcagc tgacaatgga aaaacctggg aatggaactg
    10261 gtatatgtat ttcagcagaa taagataaat ctgcctgcag tctgacaacg gaaagaacaa
    10321 ccaacagcta ctataaacgc tgtaaaaact ggctggcatc ttccctattt taccctttcc
    10381 cgtaaacccc ctcccgccgg tccttcgtat atttgcccct tgaattttag cgactatgag
    10441 taagaaagga aaagtgctgg tggccatgag cggcggtatc gacagtaccg ttacagccct
    10501 gatgctcaat gccgaaggct acgaagtggt cggcatcacc atgaaaacat gggactatat
    10561 ggccagcggt ggcagcaaaa aagaaacagg ctgttgtaat cttgactcct tcaacgatgc
    10621 gagacaggca gcggtggagc atggctttgc acattatata ctcgacatac gggaagagtt
    10681 tggtgatttt gtgatcgaga attttgtaga agaatacctg gccggccgca cccccaaccc
    10741 ctgtgtactc tgtaatacgc atatcaaatg gcgggcttta ttaaaaagag ccgatgccct
    10801 gggctgtgaa tttattgcca caggacacta tgccaaaata agacagcatg acaatggacg
    10861 ctttgtggtc agtaaagcca ttgatgatac caaggaccag agttatgtgc tatggggatt
    10921 gcaacaagat ctcctgagca gaacattact gccactggga ccttaccgta aaacagagat
    10981 ccgccagatg gcgcatgatt ttggatatcc tgaactggca aaaaagaatg aaagctatga
    11041 gatctgtttt gtgccggaca atgattaccg cggcttcctg aaaagaagag tggatggact
    11101 ggaagaaaga gtggccggtg gcaacttcat tgataaaaat ggtaagattc tggggcagca
    11161 taaaggctat cctttttata cgatcgggca acgtaaggga ctagacatag caatgggaag
    11221 acccgttttc gtgacaaaaa ttgatcctga caataacacc gtgatgctgg gcgatgaaga
    11281 ggacctggag caaaatgaaa tgatagtgag taagctgaat ttaataaaat atgacagcat
    11341 cacccctggt atggaagcag taacaaagat ccgttacaaa gacaagggta gcctgtccac
    11401 aatttatcct gaaggcgggg ttatgaaagt gcgattttac gaaaatgcaa aaggcattgc
    11461 accgggacaa agtgccgttt tttatgaagg tgatgatgtg attggcgggg gtataataca
    11521 gagagggatg ctgaacgctt aatgcctaac gctgaaggct gaaggcctat tgcataaagc
    11581 tgaatgctta atacagaata caagatccat gagggattat aaaaaatatg atgtttggat
    11641 aaaagcacat gagctagttt tgttcgtata caaagaagtg atccccggtt ttccaaaatc
    11701 agaacaattt gaactggtat cccaaatcaa aagagcagcc tattctatac cgatgaatat
    11761 agcagaagga tgtggaagaa attcagataa agattttgtt catttcctgg atatagcatt
    11821 aggttcagcc catgagctgg agtattgcgg actattaggg gcagacctgg ggttcattaa
    11881 gaaagcaaac gttgatccgc tgaatacaag ggtgaatgag gtgaaagcca aactaatcaa
    11941 cctcatcaaa gccatcagaa aataggatcc cccgctttaa gccttcagcc ttcagcatta
    12001 agcgtaaggc attaggcgtt aggctttctg catttcgcct taagctttta gccatccgct
    12061 ttaagccttt tgcattcagc attaagcctt tatctgcaat aaagcgaaat aaaatacgtt
    12121 ctaaaccggg gagttatgtt tccccgtttt ttgtttgtag atttattcgt caattttgta
    12181 tccgtgaaca aagaaaaacc acttatgaga attttttcac ctgtacgcct tagtctgtta
    12241 gctattcttt ccgctgctct cttttcctgt aatgaaaaag aagattttac tacggaagca
    12301 ttagcagatt atatcccctt aactgcaaag aaatatatca cttaccggct cgactcaatg
    12361 gtgttcacca attttggacg gaatacagag atccgtaaat accaggtaaa acatgtaata
    12421 gacaccctga tcaccgacaa cctgggccga cccagttaca gggtttatcg ttacctgagt
    12481 gactcaacag gtacacagcc ctggcaacct tcaggtacct atttcatcac tcccctgtct
    12541 gaccaggtag aactgattga agacaatctt cgttttataa aaatgcacct gccaataaga
    12601 aatggtttta gctggaaagg aaaccggttt cttccgtcag atccttatgg tactatctac
    12661 aacttcagca atgatgatag tatggaagac tgggatttct atttcgacgg ggaaccatct
    12721 tcattcagcc accggggaat taattacact gatgtgcata gtatagagca ggtggatgaa
    12781 gcgttcaatg tacctattac cagtccaacg gcttatgctg caaaatcaag atctgtagaa
    12841 aaatatgcta aaaatatagg gcttgtatac cgtgaatatg agctttggga ataccagcca
    12901 aaccccgggg gagccggcgg gccttataaa acaggctttg gcatcaccat gtggatggta
    12961 gatcataatt agtaattggt attctctaaa tttgtggttg gtaaaaaata ccggtattcc
    13021 atgaaaaaaa ttttactcct gtttatagtt gcggttactt tcattgttga atcgcaggca
    13081 caatttaccc ggtacattgt aaaacttaaa gacaaaggca acaatacgtt ttcccttgcc
    13141 aacccttccg gttatttatc acaaaggtct attgaaagaa gaacccgtta tggaatagca
    13201 attgacagca cagacctgcc ggttacacct tcgtatgtta cccagatcag aaatgtaccc
    13261 aacgttacta ttttgaatgt atccaaatgg ctgaatgctg tctccattca aacctccgat
    13321 gcaaatgcga ttacagcgat cactgcttta ccatttgtaa aaagtgttgg cggactggcc
    13381 gcaagaagta caggcgatga acccggaccc accaggaata aatttgtaac ggaagaagcg
    13441 atcaatccca tacctgaact ggcacagcgg acagaacagc ttaccggtga tttttttaat
    13501 tatggaaccg gttcctttaa tgagattcac ctgcacaatg gtgagttctt acataatatt
    13561 ggattaagag gccagggcat gcaaatcgct atgctcgacg gtggtttttt caactacaat
    13621 tcattaagag cttttgacag tgccaatacc aacagccagg tattaagtac ctgggatttt
    13681 gtatcccgca atgcgacagt tacggacgat catccgcatg gcatgcaatg tttctccacc
    13741 attgcggcta atattccggg acaatttgta ggtaaagcac ccaaagcagg ttttcattta
    13801 ttccgtactg aagatgtagc atcagaatac ccgatcgaag aacataattg ggtatgtggt
    13861 gccgagagag cagatagtgc tggctcagat attatatctt cttccctggg ttattatgat
    13921 tttgataatg cagttttcaa ttacacctac agcaatatgg atggcaatac cactatggcc
    13981 gttattggtg ctgacctggc tgctaaaaaa ggattgatgg tatttaattc tgcaggaaac
    14041 gaaggtggga atgcctggcg cttcctcgtc actccatccg atggtgatag tgtagtggct
    14101 gtaggggcag tgaattcttc cggtatagtt ggcagcttct ccagttatgg accttccggc
    14161 gacggacaga taaagcccga tatggcttct gttggtgtag ctgcattggt tcaaacaagc
    14221 agcaatacta tcggaacaag caatggtacc tcctttgcct gtcccaatat ggcgggtttg
    14281 gcaacttgtc tctggcaggg ttttcccgag tataataata tgaagatcat ccaggcattg
    14341 aaacaggcca gtaataaatt cagcacaccg gatgacagga tgggatatgg tattcccaac
    14401 ctgaaactgg cgtttacaaa cctgctgaca gaatatgcca cagcaactgc atcagcaaat
    14461 gcctgtactg tttccgtaac atggaacagc aaagacgtag caactatgaa gtacgagatc
    14521 gaaagaaaag tgccgggtga atctgtatac acaaaagtgg gagatattaa tgcacaggcg
    14581 ggaaccatac tcactaataa aagttattcc ttcaacaata cattggttgg tgtaaatccc
    14641 ggtactgttt cgtaccggat ccgccaggta gttgacactg ctgctgctac atttactgca
    14701 gtttatattg acactgccga tgtaactatc actgctggtt gttttgctac cggaaccagc
    14761 aatcagttgg taatggtgca gccaaaccct gtaagcggca gtactgttac gctggttgtg
    14821 gaaacacctt atgccattcc aactatgtca gttatgatgt atgacgcaaa aggcagcctc
    14881 gtaatgcaaa ccaaagatgc aaaaggaact ggtaaaaaac tgatcgatct ttctgtcagt
    14941 aaactttcca ctggcaaata ctatatcagt gtttatgaca atcagaaatt gttaggcact
    15001 gcagaactga tcaggttata aaggcttttg taaaagagag gcaaacaaag tgtcggccct
    15061 tttatcataa ccggtcattg atttcttttc aacttctgca aggccgtact tttcttttat
    15121 atattctaca acgaactcat tttccttttt aaataccgaa caggtaatat agagaaggaa
    15181 gcctccgggc cgcaaagagg gaataatagc cgaagtgatt ttcttttgca gcagactgta
    15241 ttcatcaatc ttttgtgcat cgaaaaaata taattgctct ggtgtgcggc tccaggtgcc
    15301 actcccactg cagggaacat cagcaatgat cagatcatac tgctgctttg caggttgtga
    15361 accagatgca gtaagatcag ccacaaaact tttgtactgt ttaatacctg cttcagcaaa
    15421 tctttgtttc aggttgtaaa gtatactctc ccgtatatcg gatacggtga ggtcaatatc
    15481 ctcaagtata tctttggcca tgatagattt accaccactg gcagcacagc agtcccaaac
    15541 ccggaaaaca ttttccttct taacctgtgc cagcaactta cccacttgtt gagaactgta
    15601 atcctgtatt acagcttctt tattcaattc gattatcgta tccactttta aagagttcgc
    15661 tactgcaata caactctcat taatagtctc aaacacaatt tcctgttgcc ggagtttggc
    15721 catcactctc tcttgattac caggccgcag ccgaagaaag aggtctggct gtatgaagaa
    15781 ggcagcacaa aatttttcat agtcaatccc ctcactaaat tcattccccc acgggaaaat
    15841 atcatttaca gaaaaagaag agtccagcaa tagaattttt ttggataatg gtaatgctgc
    15901 atgcggatct aagtctggtc tcaaatgctg aagcagctca ttggatgtta aagaacataa
    15961 aaacaagccg gcaattattc tttcctcaac cggcatttcc gctactgctt tacccaacct
    16021 gaaatagcaa taacataaat ggcttatact tttcctgtct ttggagccat actttttatt
    16081 agttgaaaaa tgctttttca gaaatagaga aaaaggctca tctcccctgt atttaccaag
    16141 aagctggata gcactgttca ggtatgaata atatcggctc accctgcaaa ataaggcaag
    16201 caaaccggct tacgcaaatg aattgataaa agcagaacta ttgataatga taggtggaaa
    16261 cagtaagctc accgggagga gttcgtatag aaaaagctga atcaggtttg cccgcagcat
    16321 tatacctgta aattatatca aggttaaaat tggcggttgg atctgcagta ttggtaaata
    16381 cagtttttaa actattatta gcattgaaca gacttgttct tgacaataaa acagcctcgt
    16441 gggataatat cagcgggttg gttttagtat cataggtata ggtttgagag gaaacaggca
    16501 atagtggtcc tccgggggta gtagcggcca tttgatcctg cctggttatg tttaccccat
    16561 cagcggaata ggtataatca tttttcagac tttggatagg aggtagaaaa ccagttgcca
    16621 ggtaatgcac atcacttata atcttaccac ttgcatcata ggagtaaacg gcactatcag
    16681 taaccgaaaa accaaaagct gtcagagaaa aaactgatga agtgtacctt gatgttgaag
    16741 tattataatt ataccgggta acaacactat caatacctgc agttgccaat gctgctgcct
    16801 tttgtacagt tgtaagaatg ataccagcag cattccggtt tattatcaat tcattgtcca
    16861 gtgtaagccc tcctgaaata cctgttgtct tttccctgat caatctgttt tgggtattat
    16921 aaaaataact gacctcggaa gagtccgtac ctgtttttga attgattctt gaaagcaggt
    16981 tattactgcc agtgccaccc agtccccaat caatttcctt ctgacaagac atataaacta
    17041 aagaaagcag tataattact aaggccagat atcttttcat gtaattgcaa aaattttctt
    17101 gtttataact caagcaatgt tttaaccggg tcttttccaa tcagtaattg ctccgggttc
    17161 tccaataatt ctttcactct caccaggaaa cttacagatt cacggccatc aacaatacga
    17221 tgatcataac tcaatgcgag gtacatcata ggtcttatca ccacctgccc gtttatggcc
    17281 attggtcttt cctgtatttt atgcatacca agaatggcac tttgtggaat attgataatg
    17341 ggtgtactca tcaacgaacc aaaaacaccg ccattggtga tagtgaaagt tccacccgtc
    17401 aattcctccg ttgttaattt actgtctctt gctttacctg ccagttccac taccatcttc
    17461 tcaatatcgg ccatactcat gctttctaca tttcgcataa ccggtacggt caatcctctt
    17521 ggtgtactca cagcaatgct gatatcagca taatcatgat acactaactg atctccgtca
    17581 atgtatgcat ttaccgatgg ccattctgcc agcgccactg cacaggcttt ggcaaagaag
    17641 ctcatgaagc ctaatcctac cccatgtatc tctttaaact tgtctttata cttcgttctt
    17701 atttccatga tactgcccat atccacctca ttaaaagtgg tgagcatcgc tgttgtattc
    17761 ttggcttcta ccaagcggcg tgaaatagtt ttacgcaggt tactcatctt ctcgacacga
    17821 acattccggc tgaacaattc agccccgtta aaggctttct taccgggatt tgataaagct
    17881 gctaacacat catctttcat gatcttgccc tggtaaccgc ttgctgttac agatttagga
    17941 tctacttttt tatcggcaat gattgctgaa gcaacaggag aggccttgat atcattggga
    18001 actgagctga caggtgcggt tgtcgttgct ggttttgcag cctccgtttt ttgagcactg
    18061 gcagtttctg cttgctgagc tgaagcaggt tttggtgctg tttcatcaat actcgccagc
    18121 aggtcgccga tcttaatagt atcgccttcg tttaccgaac caagcttcaa cacacctgcc
    18181 tgctccgcat ttacttcaaa agtagctttc tcgctttcca gttcggcgat cacttcatct
    18241 ctttccacat agtcgccatc tttcttattc catttcaata aagtgacttc gctgatagac
    18301 tctcctaccg ttggtacttt tatttcaatc ataatattct attaattcaa tttaaatact
    18361 aaatgctgtt tctattattt cagcctgttc ctgcaaatga actttattat aacccgttgc
    18421 tgttgaagca gagggttgcc tgcctattac accatagtta atgcccgtta aattcatttt
    18481 aaggaatgaa gcggcgccca tattcatagg ttcttcctgc acccagaacc aggttgcctt
    18541 actgtatttt cgttgtaatg catcaagctg tttgtagggc aaaggataga tctgctccag
    18601 cctgattatc gcaatatcat ttctgttttc cttctgctga cgttcagcca tatcaaaata
    18661 tatcttgcca cagcagaata acactttttt cactttagct gcatctgcct gtgcatcgtc
    18721 aatcacttct ttaaacccgc cactggtaaa ctcttcgatt cttgaataag aaccagcatg
    18781 gcgaagattt gctttgggag aaaaattgat cagcggttta cggaatggcc atgccagttg
    18841 acgtcttaag gcatgaaaga aatttgcagc agtagttata tttgttacca ccatatttaa
    18901 ttctgcacat aattgtaaaa accgttccag cctcgcacta ctatgctccg gcccctgtcc
    18961 ttcatagcca tgtggcagta acatcgtaac accattcatc ctgttccatt tttgttcccc
    19021 ggcagcgatg aactggtcaa tcattgtttg agcaccattg gcaaaatcgc cgaactgagc
    19081 ttcccataaa accaatgcat tcggattggc caatgcgtag ccatattcaa aacccagcac
    19141 accatattca ctcagcagag aattgtaaat cctgaacttt ccagttgctc cgggtatatg
    19201 agacaacctg ctgtatggtt tatcggtatt ttcatctctt aatactgcat gacggtgcga
    19261 aaaagttccc cttctcacat cctgtccgct cattctcaca tcttttcctt ccagtaataa
    19321 gctgccatag gcaagtaatt caccggtagc ccagtccact tttctttctg tctcaaataa
    19381 ttttattttg tcctgaagga tcttctcgac ttttcgcagt ggcttgaaat ctgcaggcca
    19441 tatcatcatg gcgtcaaaaa ctttccgaaa gtctgcttct gtaatacttg taaccggtga
    19501 tgaatcgaaa tcttccggag ttgccctgcg aagctttttc cactccagct cagggggctg
    19561 gtaatgatac ggtaatggat tctgtttcac ttcatccagt cgctcttgca gatcagccca
    19621 aaactttttt tccatttctt tggccaattg ctgtgcatct ggttcaccat tctccaatag
    19681 gtatttaata tacacttctc ttggattggg atgcttatca attaaagcat acaaatgagg
    19741 ttgtgtgaat ttaggatcat cgccttcatt gtgaccatga cggcggtaac acaccatatc
    19801 aataaaaaaa tcactgttaa attcctggcg gaaacgggtt gcaatttccg cacatttgat
    19861 taccgcttca ggatcgtctc cgtttacatg aaataccggt gcctggatag cagctgctaa
    19921 tgaagtacaa taatcagaac ttcttgcatc atcaaaatct gtggtaaacc caatctgatt
    19981 atttattaca aagtgaatgg tcccaccagt gtagtaacct ttcaggtagc tcatctgtaa
    20041 cacttcgtac acaactccct ggccggctac tgaagcatca ccatgaatca gaattggaag
    20101 tattctgtca aactcactgt gatacatgac atctgcttta gcccgggaaa aacctaccac
    20161 cacggggtta acagcctcca ggtgtgacgg attaggcatc agcttaagat gaatggtttt
    20221 gtcattcatc gttttcactt cactgccata acccatgtga tattttacat cgccactacc
    20281 catggtttga tccatcttgg cagttccctc aaactcactg aatatctgtt catatgtttt
    20341 tcccatgatg ttggccagga tatttaacct gcccctgtgt gccataccaa ttactacttc
    20401 atgtacatca ttatctgcgg ctgtatttat gatcgcatcg agagctgcaa ttgtagtttc
    20461 acccccttcg agtgaaaacc ttttctgacc aacatacttg gtatgaagaa atttttcaaa
    20521 catcaccccc tggttaagtt tttctaatgc tcttcttttc ttatccagtg gtaagggttc
    20581 cagcatcctc ttctcaatct cgttggtgag ccagtcaatt tttttctgat cgctgatgta
    20641 tttaaactca gcacccacac ttgatgcata gcatttttca agatgagcta atatatttct
    20701 taaggagttt gtgcccagtc cgataagctg gcctgcatga aattccttat ccagatcagc
    20761 ctcattaaat ccataaaacg caaggtcgat attggctccc ctgtcctttc gttgacggat
    20821 aggatttgtt tttgcaatca aatgaccttt attcctatac cccactatta aacggtatgc
    20881 ctttatctcc cgcatccaat ctacctcggc acttattact tttccggttg caacgggcat
    20941 ttcttttcca ttacccaccc ccgtcatgct actattgcca atagcaaaat caaacccctc
    21001 aaagaacttt ttaaattcgg ggtcaacact ggtagggtca tttacaaaat cacggtaaag
    21061 gttttcgata aactccgggt gagaattagt gatataggaa aaatccttca tgaaaagagg
    21121 tattaaaata caagtgaaaa ttgggccaca aatatcgctg aaaaaacgga aatccctgcc
    21181 tcattatgag ggatttagaa agccctgaag cacaatggga tattctttgc tatagtccaa
    21241 tttgcctgct ggctctttac taaagcagga ggattagagc acttgcaatt attatgacct
    21301 tttaggaata aaacctccgg aaatttggtt aataggtgcc ccaaccctac ctttgcaatc
    21361 cttaaaattg atatagatgg ctaatcacag tgcaacaaag aaggatgccc gccaggcagc
    21421 taaacgtcgt gacaggaaca aatactatgg caaaacgacc cgtaacgcta tccgtgacct
    21481 gaaagcggcc cctgtaaagg aaatgggtga aaaattccct cttgttgaaa gcatgattga
    21541 caaactggca aaacgtggtg ttattcacaa gaataaagcc gctaacctga aaagtaaact
    21601 cgcaaaaaaa gttaattcag ctaaataagc tgtactaata tctcttttaa aaggcatctc
    21661 agtttgggat gcctttttat ttttagtagc ttcctgccaa atagtatttt taagcaacaa
    21721 cccacgatcg tatgcgacac ttctttttga tattttttct ccttgtttcc tttattgccg
    21781 gggctcaaaa gctaaccacc agatttgaac aatccaacag tacccaaact cctgactatt
    21841 ttgaaataat tgattggtgg aaaaaactgg atttacaatc aggcaaaata aaaatgctga
    21901 caatggggat tacagatgcg gggtatccat tacatctggt cgttgtctca aataatgggg
    21961 attacaattt cagcaatatc cggaaaaata ataaaaggat catactgatc aacaatggta
    22021 ttcaccccgg cgaacccgat ggcattgatg ccagcatgtt actggcaaga gacattgttg
    22081 ccggtaaata caaaatacct gataatatag tattggccat tatcccggtc tataatattg
    22141 gtggttgttt aaaccgaagc gcaaactaca gggtcgatca aaatggtcct gaagaatttg
    22201 gttttcgtgg taattcacaa aatcttgatc tgaaccggga ttttattaaa tgtgattcga
    22261 gagatgccag ggctttcact gaaatatttc atctaacaga ccctgacgtt tttgtagata
    22321 accatgtaag caatggcgcc gattaccagc atgttatgac attgcttact acacagaata
    22381 gcaaactggg cggcacgatg ggagaatata taagcaaaga atttgagccg gctttatact
    22441 cgttaatgaa aacaaaggga tttgatctta tcccttacgt caattctttt ggtgatacac
    22501 cagagaacgg atggccggaa tactgggata gccccaggta tgccagcggc tatgctgcct
    22561 tatggcatac attctctttt gttccggaaa gccatatgtt gaaaccctac gatcaacggg
    22621 tgaaagccac ttatgccctg atgcaatcgt ttattgaatt caccaaaaag aatagtgaac
    22681 agataaaaag tatacgtaat caaacaaaaa aaagtgttga aactgcaaac gagtttcctg
    22741 taagttggtc attgaaccgt tcacaattta aggaagtaat gtacaaagga tacgaagctg
    22801 gacgaaaacc cagtgagatt tctggcttgc ccagattgtt ttacgaccgg aataaaccat
    22861 ttgaaaaaaa ggtaaaaatc tacaacaact ttgcacccaa aacaataata aaaaaaccaa
    22921 aagcatatgt aattcctcag ggatggtgga aagtgattga gctcctgaaa ttaaacaagg
    22981 tgcagatgag tcagcttaaa aaggatacaa tcattgaagc agaagtttat aaaatagagg
    23041 attacaagac tactccccgc cagtatgaaa tgcaccactt aaacagtgat gtaaaaacca
    23101 caactacaat gcaaacaatc cggtttctta aaggcgactg gtatatacca atgaaccagc
    23161 ctgctaatag gtttttaatg gaaactttgg aaccacaagc tgaagactcc tatttcgcct
    23221 ggaattattt tgatgccatt cttggtcaga aagaaggata ttcagcctat gtttttgaag
    23281 acatagcagc cggttatctg aaaaacaata ctgaattaaa agtaaaattg gaacaaagaa
    23341 agcaatcgga tactgcattc gccggcaatg caagggcaca acttaatttt gtataccaaa
    23401 actcttcctg gtatgagccg gctcatatgc gttatccggt ttatagaatt ttaaaatagt
    23461 tattcgttga taaaattctt atctgataaa gttttatcag ctctgtcaat ttttttaaac
    23521 aatattcttc caagcacaag actgataagg agcacagcaa tgctgagata gcctaaccag
    23581 tcaaatcgtt gaattttacc ggttttatct tttaaaacaa taacacccgc agccatggaa
    23641 gcaataaagg ttcccagttg ctgcatactg ccattaaaac tcataaaact gcctctttgt
    23701 tcagtcttta caacctcact aaccattgct tgtgcagtaa ctgccctacc agtagccaat
    23761 ataaaccata cggcaaaaaa tgccagtaca atggaaaaat gaacatctgg catattggta
    23821 attaccaata ccatgaatag agacaagaaa acagagattg aaaatacagg cagtttaccc
    23881 ttcttgtctg aaaaacgccc caggaatatt gccgctgcaa aagaagaaat accacctacc
    23941 aggtaaacca taggcgtcag atcctttgaa aaccctttat taaattccag gtaaggattc
    24001 aggaacggta taatcagaaa atgtcccatc attaacagcc ctgaaaatag taatgcttgc
    24061 tgctgtgctg ggctttcaaa aaccttacct aacaccctcc aggtggattc tctattccca
    24121 accgatttta aatgagctga cataccggga ataaatttgt aaatcagtgg tatcaaaaca
    24181 attcctattc ctccaacaag caggaatggg acatgccagt taaaattgaa aagattagtt
    24241 aggtacaatg aaaatggaac acctaatgtt gaagcaacag caaaagcact cattacagct
    24301 cccattgctg tacctcttct ttcatataca aaaagatcgg ctatgattga caacacctgt
    24361 gctccaataa taccaccaaa taaaccagcc aatattcttg atcccaggag tagaccataa
    24421 gtaggagcta tgccacatgc caatgttcca attataaaac cggcataaga aacaagtaat
    24481 acttttttcc tgtcaaatct gtccacaata aaagctgctg tgaagccgga tacagctgcg
    24541 cttatagaat atgcccctac cagtaaactg aactgccagg gagaaatatg gaagtatggc
    24601 attaaataat tacccaatgg catcataatc ataaaatcga gaatatgggt aaaattcaat
    24661 ccggccagaa gaaatagtat aattctttct ttttgattca ttgattcgtc agaaatgctt
    24721 tagtaaaaat gagggataga gatatgttga caaagattac tttaatttat ataacaaaaa
    24781 agcctcccag aataaactga aaggctttta aatgaatatg gcagctacct actctcccgc
    24841 attgttgtgc agtaccatcg gccatgaggg gcttaacttc tctgttcggt atgggaagag
    24901 gtgaacaccc tcggcaaaac caccataaga tcttaa
//
LOCUS       DAORTI010000043        24690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1587_length_24690_cov_8.268399, whole genome shotgun sequence.
ACCESSION   DAORTI010000043 DAORTI010000000
VERSION     DAORTI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24690
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1587_length_24690_cov_8.268399"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PLO70_07530"
     CDS             complement(<1..1521)
                     /locus_tag="PLO70_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQZ74351.1"
                     /translation="MQALKRFWLLLLFGSFSVQLSAQINPEQPMPVDPKVKIGRLSNG
                     LTYYIRQNKKPEQRVELRLVVNTGSVLEDEDQQGLAHFMEHMNFNGTKRFPKNELVSY
                     LQSIGVEFGADLNAYTSFDETVYMLPVPTDKPGLVDKGLQILEDWAHNALLDSLEIEK
                     ERGVVIEEWRLSRGADERMMKQTLPVQYQGSKYAERLPIGKIESLRNFSHAALKRFYT
                     DWYRPDLQAIIIVGDIDVNDMEQKVKETFGDIPAATAPRKRETFPVPDHTEVLTVVAK
                     DKETAFPSVEVLFKKDPLPETTIGDYVRYMNEMLFTGMLNNRFREVTLKPNPPFVGAG
                     SYYGESYARTKDAYQLFANTSDTGMARSLYALMEENRRVLLYGFTPSEFELQKKQIQS
                     FYDRIFNEREKEESYKYADEYAGNFLVNEPIPGIEWEYDFVKQHLSSVTLQDVNKLAA
                     QWITKNNMVVTLNAPEKEGVKIPSAAEVSSVLSAVDIAAIEPYKEKVLATSLIEPGK"
     gene            complement(1622..2200)
                     /gene="msrB"
                     /locus_tag="PLO70_07535"
     CDS             complement(1622..2200)
                     /gene="msrB"
                     /locus_tag="PLO70_07535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQZ74352.1"
                     /translation="MSTIARLILLVVTTGLLQQCSYSQNNNSTTNTAKMDTTKKNNPV
                     YSTSDTSKINMSEEEWKKILPSDVFYIARQKGTERPWTSKFEKFKEVGTYYCAACGNA
                     LFKSDTKFDSGCGWPSFYEPISKGSIIYTPDNTHGMERTEVQCGRCKAHLGHVFDDGP
                     PPTGLRYCINGVILDFAKAKDAEKNYKEKPQQ"
     gene            2374..2958
                     /locus_tag="PLO70_07540"
     CDS             2374..2958
                     /locus_tag="PLO70_07540"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQZ74353.1"
                     /translation="MFNLILFGPPGSGKGTQSDKLVEKYGLKHLSTGNLLRQEIADKT
                     PLGIEAKSFMDKGQLVPDEVVIGMVDSYFDQHKGAKGFLFDGFPRTVAQAVALDKLLD
                     LKNTSIASVLALEVSEEELVKRLLNRGKTSGRSDDTNEEVIRKRFAVYINETTPVAEH
                     YKKAKKFIAIKGEGSVNEIFSALCKSIDKKMDRD"
     gene            complement(2968..3900)
                     /locus_tag="PLO70_07545"
     CDS             complement(2968..3900)
                     /locus_tag="PLO70_07545"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQZ74354.1"
                     /translation="MPFTEKQYRQRISLGQSDNTLTRLIIINLVVFITLALIKVFYYF
                     LFREEAIVASRFSNEALKWVVLPADMNNFASRPWTILTAMFTHVGIWMTLANMLWLWT
                     FGYIMQDLTGNRKIIPLFIYGGLAGAIAFLIAVNLLPAAQTPANAVYFGASASITAIA
                     VATTMVSPGYRIFPMLNGGFPLWILTAFYLVISIATVPYYSVEAYMPQVAGAFMGFLF
                     IWLIRRGYDWSDWMSNFFDWVNNLFNPDKPRRGQKSIKEQLFYKSSSDPYKRTPNVTQ
                     QRIDDILDKINQKGYNSLTAEEKDLLKRASEDEV"
     gene            complement(4007..4750)
                     /locus_tag="PLO70_07550"
     CDS             complement(4007..4750)
                     /locus_tag="PLO70_07550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQZ74355.1"
                     /translation="MSDFRYTRPDGFPPIVKNLIIINVLVYLAQILLANQFAITERFM
                     LWPLMPDKLHELLVQSGALDASQQFQPYQIVTHMFSHAPPPMLFHILFNMFTLWMFGR
                     VLENVWGGKRFLFFYLACGLGAAALHLGMQYFRCEQLLQAFNENDIEAVKSHLGAAAP
                     ALGASGAVMGVMMAFAYLFPNTELYIMFIPFPVKAKWAMLGLAAIDLFGGVARISGDN
                     IAHFAHLGGAVTGFFIVLYWNKTNRRSLY"
     gene            4948..5331
                     /locus_tag="PLO70_07555"
     CDS             4948..5331
                     /locus_tag="PLO70_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74356.1"
                     /translation="MPCRAQDTLTAFTKQLSYRWPSWRLDGKKIHLSKLGFEILKVEA
                     AVPWYQKSKNNETKAYLAMGSAAILAILGSPETDITSPRFGKNNLGYSIGSLVAGGVS
                     IFFYSRARKFLKKAVRIYNEKRLIY"
     gene            complement(5328..6737)
                     /gene="rlmD"
                     /locus_tag="PLO70_07560"
     CDS             complement(5328..6737)
                     /gene="rlmD"
                     /locus_tag="PLO70_07560"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQZ74357.1"
                     /translation="MRKKKKNIVLEKILVEDYAAEGKCLSRVDGKVIFIENVVPGDVV
                     DVRLLKNKKDWAEGVPLQFHSYSGERVTPFCSHFGVCGGCQWQMLPYEKQLQYKSKQV
                     GDVLQRIGKIPLPPIQPIIGGAETKYYRNKIEYTFGNKRFLLKEEIADETINGQQDVA
                     GFHAKGFFDKVVDIEQCYLQAEPTNAIRLAVKAFAKSNGWPFYDIRNHVGFLRTMQVR
                     LCTTGELMVNIVVAENDEEKINKLMEYVMELFPAITTLLYTINTKWNDSLYDLEPVTW
                     HGKGYVIEKLEDFQFKIGPKSFFQTNTKQAEQLYKVTRDFAELTGHETVYDLYCGTGS
                     IGIFVSEKAKKIIGVEMIEAAIEDAKENAMLNKLDSTSFFAGDVIDICNDEFFAAHGR
                     PDVIITDPPRAGMHEKLVQKILDIAAPVVVYVSCNPATQARDLNLLDEKYAVTRVQPV
                     DMFPHTHHIENVVQLKLKA"
     gene            6920..8353
                     /locus_tag="PLO70_07565"
     CDS             6920..8353
                     /locus_tag="PLO70_07565"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HQZ74358.1"
                     /translation="MKRFTFLLFLLSSLAATAQTGYDIKVTFKPFKNQYIYLGHYFGK
                     TYPIIDSAKLDNNSVAIFKGNKKLPGGIYLVGYPNKAGFFEILVDKQQQFSIKADTAT
                     LSKGAQFENSPDNVLFVNYQQYMSEKGKKIAALQQQLKTAAGKTDSTKITDELANEDK
                     AVTTYREDIIKKNPAAMLSTLLVTMREPELTGALKNPANKEDSVLAYNFYKSHYWDGV
                     NFWDGRLAYTPFFEDKIDKYFSQLVVPNPDSVIKEIDWMLGYASINEEMNRFLLIKFV
                     NRYLNQKYMWEDAVFVHLFEKYFSQKTYSWLNETGKKTITDRAYSLMANILGTPASDV
                     ELPDPNGTPVSLYALQAEFTIVAFWDPTCGHCKEVLPKLDSFYRAKWKADGLKMYAVA
                     KETDGTKKDWLNFISDNKLQEWSHVYYSKEADKSRTDNGIPGYSQLYDVQSFPTLYLL
                     DKEKRIIAKKLTYQQLDEIVQLKKKGQ"
     gene            8377..10323
                     /locus_tag="PLO70_07570"
     CDS             8377..10323
                     /locus_tag="PLO70_07570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQZ74359.1"
                     /translation="MNRKFIFLIIACGNMIAASAQTLFTYGNYAADAKDFLRAYNKNN
                     SAAVTNKAKSISDYLDLYIKSKLKVREAYDRGYDTLPNIRMEVENLRAQIAENYMTDP
                     EIMNRLEKEAFQRSQKDIRTAHIFIAFKNGSGFVDTLAAQKKRDEIIQKLQKGEDFLL
                     LAKQFSDDPNAKLNNGELEYITVFTLPYEFENAVYNTPAGKYSSPVRSKIGYHIFKNL
                     GERKSAGKMKAQQILLATPPGADEATKKQLANLADSLYKRLLAGDEFGQLAKSFSNDY
                     ISAANNGIMPDIGVGQYDAVFEKVVWSLAKDGAFSKPFQTAHGWHIIKRIAVKPTVTD
                     PGNKDFKQELQQKIMADSRWRASKDFVYKQVTEKAGVKKFMYDDAALWALSDSLLDRR
                     PMREIGRAITSATPLFSIGKEVYDAAAWINYAGTYRFRQDGTGAKPHEQVREEWMQYA
                     MFNYYKKHLEDFNEEFRSQMAEFKDGNLFFEVMQQEVWNKAQTDTVALLALYNKNKKE
                     YTWKQSADVVIFFCSDQAIAKTVYDKVKANPAEWRKVVDMYSEKVLADSARYEWSQIP
                     NLNKMLPRAGMITTPLLNATDNTASFAYIVNAYPQTSQRSFNEAKGLVINDYQALLEK
                     EWDEALRKKYPVVIDQKVLSDISK"
     gene            complement(10419..11369)
                     /gene="metF"
                     /locus_tag="PLO70_07575"
     CDS             complement(10419..11369)
                     /gene="metF"
                     /locus_tag="PLO70_07575"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HQZ74360.1"
                     /translation="MKVTDHIAQAKDTLISFEVLPPLKGKGIEALYKHLDPLMEFKPS
                     FINVTYHRSEHVFKKRVDGSFEKVVIRKRPGTESICAAIMNKYNVDTVPHLICGGFSI
                     NDTEDALINLRYLGIDNVLVLRGDAAKNETAFEPEPGGHKYASDLLKQVVNLNAGIYL
                     EDELKGTHKTEFCIGVAGYPEKHFEAPNMETDLQYLKQKIDLGADYIITQMFFDNSKF
                     HAFVKSCRDAGITVPIIPGLKPIYSKKQLTVLPKTFHIDLPTDLSSEVLRCKTDEEVA
                     HVGAEWLLMQSKELKKAGVPVLHYYTLGRPQLVANVVKEL"
     gene            11458..12243
                     /gene="lptB"
                     /locus_tag="PLO70_07580"
     CDS             11458..12243
                     /gene="lptB"
                     /locus_tag="PLO70_07580"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQZ74361.1"
                     /translation="MAITIETKDLVKKYGSRTVVDHVSFEVKQGEIVGLLGPNGAGKT
                     TSFYQVVGLIKPDEGKVFLNDQDITSLPMYKRAKMGIGYLPQEASVFRKLSVEDNIAA
                     ILEMTNLPKQVQRDKLESLLNEFRLQHVRKSNGDVLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGIDPIAVEDIQAIVAKLKYKNIGILITDHNVTETLSICDRAYLLIEGKIFKH
                     GTAEELADDEQVRKLYLGRNFELKRKDWLHEEAFNSINKLQDQ"
     gene            12345..13337
                     /gene="add"
                     /locus_tag="PLO70_07585"
     CDS             12345..13337
                     /gene="add"
                     /locus_tag="PLO70_07585"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HQZ74362.1"
                     /translation="MDYQKLPKIELHLHLDCSLSYKVVQQLDPAITYEEYQQSFIAPP
                     RCTDLADYITRAVKGYELMQTKEQLRLVTLDLFDQLKADNVIYAEIRFAPLLHIAKGL
                     TPAEVVQTVNDAVTEGIRLTGVQAGIILCTLRHYYEEQSMETVELVKQFKGTHVVGFD
                     IAGDEAGFPIDSHVQAFRYAMDNDIPCTAHAGEAKGPESVWETLQHFHPSRIGHGVRS
                     AEDEKLLDFLKNKNIHLEVCPTSNIQTNIYENIELHAADKIYRHGVSMSINTDARTVT
                     PTTLSREYSILEETFNWNKEHYLKCSMQAVAHCFASNRLKEELRIKFRTAYELL"
     gene            13395..14933
                     /locus_tag="PLO70_07590"
     CDS             13395..14933
                     /locus_tag="PLO70_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQZ74363.1"
                     /translation="MPATMKLLSPAISSLARMRLWRIEAWKNNPLDAQREVLQELVTS
                     AQYTEFGRKYNFSELFNVKAFKEAVPIHEYDDLKPYIERIMQGEQNILWNTPIYWFAK
                     SSGTTSDKSKFIPISEESLQDGHYKASKDVLSLYYQFYPESELLTGKALVLGGSHNIN
                     PLNNDAQYGDLSAVLLQNSPFWGHWLRTPDLSIALMDEWESKIEKLAENTIKENVTSI
                     SGVPTWTLVLFKRILEITGKKTMSEVWPSLELYMHGGVSFTPYKEQFQKLIGKNINYL
                     EMYNASEGFFAAQDIPGQDGMLLFTDHGIFMEFMPVNEYGKTKPQTVGLQDVEIGRNY
                     ALIISTNGGLWRYLVGDTIQFISLRPYRVKVSGRLKHYINAFGEEVIVDNTDKAIAVA
                     SAKTGVVVNDYTAAPVYFSDNANGAHEWLIEFEKEPENLAVFTQELDAALKAINSDYE
                     AKRYKSIALGLPVVHSLRKGVFAEWLRCKGKLGGQHKVPRLSNERKYLEEILQVNTIT
                     FDNQ"
     gene            14944..15699
                     /gene="fabG"
                     /locus_tag="PLO70_07595"
     CDS             14944..15699
                     /gene="fabG"
                     /locus_tag="PLO70_07595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQZ74364.1"
                     /translation="MKLLEGKVAIVTGAARGIGEGIALKFAAQGANVAFTYVSDSSAE
                     KAAALEAKLTAMGVRAKAYKSNAGDFAQCEAFVNDVLKEFGTVDICVNNAGISKDNLL
                     LRMTPEQWNDVIQVNLNSVFYMTKQVIRPMMKAKSGSIINMSSIIGEIGNAGQSSYAA
                     SKAGIIGFTKSVAKELGSRNIRCNAIAPGFVETDMTSYLKEGEAADKYKAGIPLGKFA
                     TAEDIANATLFLASDMSSYITGQTLSVCGGLNI"
     gene            15706..16236
                     /locus_tag="PLO70_07600"
     CDS             15706..16236
                     /locus_tag="PLO70_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HQZ74365.1"
                     /translation="MKIEIVKGDITKTAVDAIVNAANSSLLGGGGVDGAIHRAGGPAI
                     LEDCRKIIAKQGGCKTGEAVITTAGKLPSKYVIHTVGPVWNGGQNDENKKLASCYFNS
                     LSLAVEHGCKSIAFPNISTGIYGFPKEKAAVIAIETITTFLNSNNFIGKVLLICFDDE
                     NYTLAKRELERLSPGV"
     gene            complement(16220..16855)
                     /locus_tag="PLO70_07605"
     CDS             complement(16220..16855)
                     /locus_tag="PLO70_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQZ74366.1"
                     /translation="MQTFDSANFHLLCDKLTRKDRELKGIITQHGYPPLWTRPNTFQT
                     LILTILEQQVSLAAAYAAFKKLKQKIGYVTPAKILQLTDEEMRACYFTRQKMGYARGL
                     AEAVQLKKVQLKKLRALPDEEVRSELIKLKGIGHWTIDVYLMHALQRTDLFPLGDIAL
                     VNSLKEIKQLHPHISKEEMLKIAAPWRPYRTIAAMILWHAYIKKRNIKLRD"
     gene            complement(16855..17613)
                     /locus_tag="PLO70_07610"
     CDS             complement(16855..17613)
                     /locus_tag="PLO70_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQZ74367.1"
                     /translation="MTTAYVRSELHKGIRIIEFFHPQSNSLPGKILEELAQAIHAAGN
                     EEETKLIILRSAGEKAFCAGASFDELSAIRNKEEGLKFFTGFAHVINAMRICPKLIIG
                     RIHGRCVGGGVGIAAAVDYAIATEAAEVKLSELAVGIGPFVVGPAVERKIGMSAYSQL
                     AIDATRWRSAEWARKRGLFAELYATVEDMDEAISRLGYLLVHSSPEAMAEMKKIFWKG
                     TEHWDKLLVERAAISGKLVLSDFTKNAIETFKKK"
     gene            complement(17632..19707)
                     /gene="paaZ"
                     /locus_tag="PLO70_07615"
     CDS             complement(17632..19707)
                     /gene="paaZ"
                     /locus_tag="PLO70_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HQZ74368.1"
                     /translation="MTKLENYITGNWITGDGEGQLLYNAVTGAPVAAASTKGLDFKSI
                     IEYGRTTGNPALRKMTFHQRGNMLKALALHLRNHLEKFYTISYQTGATKADSWVDIEG
                     GIGNLFANASLRRKFPDEVFCIDGESHNLSKNNTFMGTHILVPKEGVAIHINAFNFPV
                     WGMLEKIAVNLLAGVPAIVKPATVTSYLTEAFVKEIIASKILPEGALQLICGSAGDLL
                     NHVTSQDIITFTGSASTGSMLKKTPAILDNNVPFNMEADSLNCIVLGADVTPDKPEWD
                     IFIKEIRKEMTLKAGQRCTGIRRIFVPENKMEDVWKAIGTSLSQTAIGNPLNEKVRMG
                     SLAGQTQRNEVKEQVQKLLASSQIVYGSLDSVEVIDADANKGAFMSPVLLLNEKPHSS
                     YEPHNIEAFGPVSTIMPYKNMDDAIVLSKLGKGSLVSTIVTADYKEAKQYVVGAASHH
                     GRILVLNNECAKESTGHGSPLPLLVHGGPGRAGGGEEMGGLRGVKHYLQRTALQGSPT
                     TITAITNVYQPHANGKDPGKHPFQKYFEELQIGDQIITEKRVITSEDIDRFADLSGDH
                     FYAHIKTTDFTGTMFEQQVAHGYFIMSIAAGLFVDSYEKNPVLLNYGIDELRFTKPVY
                     PGAEIHIRFTCKEKLPNDKRVIEKPEDFKRGDDIDKGIVKWLVEMIDEVNEVTGLATI
                     LTMVAKKIQ"
     gene            19825..20211
                     /locus_tag="PLO70_07620"
     CDS             19825..20211
                     /locus_tag="PLO70_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74369.1"
                     /translation="MKKIVLLIMAFATLVIANPVQAQEKAKWNEKEAFHEIMSKTFHP
                     AEEGKLEPIKSRSAEMVDIATAWKNSTAPEGYNQKAIQKNLKSLVKDAKKLNKLVKKN
                     AADADIKAQLVELHTLFHEITEKCEH"
     gene            20223..20711
                     /locus_tag="PLO70_07625"
     CDS             20223..20711
                     /locus_tag="PLO70_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HQZ74370.1"
                     /translation="MLKKILIAFLAMVIITACNNEKSGEDHTGHGPQKEAPKNAADSL
                     YNDVMAGHDEVMPKMGKIRGAQKEAQLMLDSIAKLPAKAQTAAAALKAKLESLINDLN
                     YADFAMDKWMVEFNMDSAKDNIEQRVKYLAEEKLKVTKVKEAVLGGLAKADSLLKAKG
                     IK"
     gene            complement(20704..21183)
                     /locus_tag="PLO70_07630"
     CDS             complement(20704..21183)
                     /locus_tag="PLO70_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74371.1"
                     /translation="MSLEKENFLRTKLVPLLQRLDPSTSPKWGKMNVQQMIEHYGGDA
                     VRNASGRLKIDTIITPPENLDRMREFMMSDKPFKENTKNPLMGEEPAPLVHKTVQGAI
                     GALQLELIYFFEAFEKNPALITRNPFFGDLNFEQNVQLLFKHALHHLRQFGVEPETI"
     gene            complement(21397..21726)
                     /locus_tag="PLO70_07635"
     CDS             complement(21397..21726)
                     /locus_tag="PLO70_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74372.1"
                     /translation="MKIEFFFTIIALVAVSISCRQRAEIKHIIINELGKDTIINIKTD
                     KTYPTTLKVDIKGSADDTFIISGFKIKGGNVDTFWHTDWYKKIITINYQAYKATKGKL
                     TVNFLLY"
     gene            21920..23041
                     /locus_tag="PLO70_07640"
     CDS             21920..23041
                     /locus_tag="PLO70_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQZ74373.1"
                     /translation="MSSKLRLFTKRFFIYSNVIVVFIFILSCLVPYLNPQTWWFISFF
                     GLAFPFLLLLVIFFLLGWLMILKPRLALISFVALLVGIKSIAVFFAFHNPRAFNYDKE
                     PGTLRVVSWNVARFIELKRNTNKGSETRLKMFELLKQQNADVLCLQEFHTSTNPEYYN
                     NIEKIQKELGYPFYYFSFDEDGDNHYYSSIIFSRYPIVDSGRIRYPRPTLPDVLLHAD
                     IKVNKDTLRFYTTHLQSLQFGKSDYRSIEKIKAAEDSLLPSSRSILSKLKRGISNRSI
                     QADIVNEVIGNSPHPVLVCADLNDVPNSYTYFTVKGDMQDAFLKKGTGIGRTFSGLSP
                     TLRIDFIFADNAIKIKQFRRVTKKYSDHYMLVADVELKK"
     gene            23242..>24690
                     /gene="cysS"
                     /locus_tag="PLO70_07645"
     CDS             23242..>24690
                     /gene="cysS"
                     /locus_tag="PLO70_07645"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQZ74374.1"
                     /translation="MSQLKVYNSLTRQKEIFKPITPGHVGMYVCGPTVSGESHLGHAR
                     PYITFDVVYRYLTYLGNKVRYVRNITDAGHFEEEGREAVDKIATKAVLEKLEPMELVQ
                     KYTNLFHWAMAQFNTISPSIEPTATGHIVEQVEMIKQIIEAGFGYEVNGSVYFDVKKY
                     AASHDYGKLSGRVIDDLLETTRELEGQEEKRDRADFALWKAAAPEHIMRWQSPWGVGY
                     PGWHIECSAMATKYLGAQFDIHGGGMDLLFPHHESEIAQSTICNHTAPVRYWIHNNMI
                     TINGRKMGKSYNNVIKLTELFSGNHPILEKAYHPMTVRFFILQTHYRSTLDFSNEALQ
                     ASEKGLKRLWEAHEKLQAMHYEPQEGTTDPELESRIPKLVAEFNEFMDDDFSTAKVMA
                     NMFEIVPVINSMKDKTIPVSAISKQTFELLQGSFKTWLEDIFGLKSVSEADNARLQGV
                     MQLLIDIRKEAKGKKDFVTSDKIRNQLAQLGIT"
BASE COUNT         7226 a         4988 c         5310 g         7166 t
ORIGIN      
        1 tttgcccggt tctatcaaac tggtagcaag cactttttct ttgtagggtt caatggctgc
       61 aatatctact gccgacagaa cactacttac ttctgctgct gaaggaattt tgacaccttc
      121 tttttcaggc gcatttaagg ttaccaccat attgtttttg gtgatccatt gcgcagccag
      181 tttattgaca tcctgtaatg ttactgatga caagtgttgc tttacaaaat catattccca
      241 ttctatgcct gggataggtt cattcacgag gaaattacct gcatattcat ctgcatattt
      301 gtaagattct tctttttccc tttcattgaa tatgcggtca taaaaacttt gtatttgttt
      361 tttctgaagt tcaaattcag agggggtaaa tccatagagc aatactctcc tgttttcttc
      421 catcaacgca tataaagaac gggccatgcc tgtatcactg gtgttggcaa atagctggta
      481 agcatctttg gttctggcat aactttcacc atagtaggat ccggccccca caaagggtgg
      541 attgggtttt aaggttactt cccggaaacg gttattcagc atgccggtaa acagcatttc
      601 gttcatataa cggacataat caccaatcgt ggtttctggc agcggatctt ttttaaatag
      661 cacttcaaca gaggggaaag cagtttcttt atcttttgcc actacggtta gaacttcggt
      721 gtgatctggt actggaaatg tttccctttt tcggggtgca gttgcagcgg gaatatctcc
      781 aaatgtttct ttcactttct gctccatatc attcacatca atatcgccta caattatgat
      841 tgcctgcaga tcgggccggt accagtcagt ataaaaccgt ttcaaggctg catgtgagaa
      901 attacgaaga gattcaattt tgccgatcgg taatctttct gcatattttg aaccctggta
      961 ctgtaccggt aatgtctgct tcatcatcct ctcatcagcc ccgcggctca gtctccattc
     1021 ctctatcact acacctcttt ccttttcgat ttccagcgaa tcaagcaatg cgttgtgtgc
     1081 ccagtcttcc agtatctgaa gacctttatc taccaggccg ggtttatctg tgggaacagg
     1141 aagcatataa accgtctcat caaaactggt ataggcattc aggtcagctc caaattcaac
     1201 accaatgctc tgcaggtagc ttaccagttc gttcttcgga aaacgtttgg tgccattaaa
     1261 attcatatgc tccataaaat gggccaggcc ctgctgatcc tcatcttcca gtacagaacc
     1321 cgtattcact accaggcgca gctctacccg ttgttcaggt tttttatttt gcctgatata
     1381 ataggtgaga ccgtttgata accggccaat ttttactttc ggatcaaccg gcatcggttg
     1441 ttccggattt atttgtgctg ataattgtac actaaaactg ccaaacagca gtaacaacca
     1501 aaatcttttg agagcttgca tacacataat tttactacag taaatttatt tatatggtat
     1561 tgctttagga aataaaaaat cccggcggtt gattgccggg atcaatcttt tcataatcac
     1621 cctattgctg tggtttttcc ttatagtttt tctctgcatc ctttgccttt gcaaagtcaa
     1681 gtataacgcc atttatgcaa tagcgtaaac ccgtcggggg aggtccatca tcaaatacat
     1741 gacccaggtg ggctttgcaa cggccacatt gtacttcggt tctttccatg ccatgcgtat
     1801 tgtcgggagt gtaaataata ctccctttac tgattggttc ataaaaactc ggccagccgc
     1861 aaccactgtc aaatttagtg tcacttttaa ataatgcatt accacaggct gcgcagtaat
     1921 aagtgccaac ttccttaaac ttttcaaact tactggtcca gggcctttcc gtacctttct
     1981 gacgggcaat ataaaagaca tctgacggaa gtatcttttt ccactcttcc tcgctcatat
     2041 ttattttgga ggtatcagat gtagaataaa cggggttatt ttttttagtg gtatccattt
     2101 tggccgtatt agtagtggaa ttattgttct gtgaataact gcattgctgg agcaatcctg
     2161 tagtaacaac cagtaatatt agtcttgcta ttgtgctcat attattgttt aacaaattta
     2221 cgcaaacaag gttggaccgg tttgggcttt agtaacaact tagatttgtt aacattttga
     2281 tggcaaataa catttcagcg aaatgtaggc ggaagataga tattttaacc cctgtacatt
     2341 atatttgtcc atacaccaag attgataatg attatgttta atttgatctt atttggccct
     2401 ccgggaagcg gaaaaggaac tcaatcggat aaactggttg aaaaatatgg gttgaagcac
     2461 ctttctaccg gcaatttgtt gcggcaggaa atagcggaca aaacaccgct gggtattgaa
     2521 gctaaaagtt ttatggataa aggccaatta gtgcccgatg aagtggtaat tggcatggtg
     2581 gactcttatt ttgatcagca taagggtgcc aaaggatttt tatttgatgg tttcccccgt
     2641 acagtggcgc aggcggtagc gttggataaa ttgcttgatt taaaaaatac aagcatagct
     2701 tcggtattgg cactggaagt aagcgaggaa gaattagtga aaagattgct gaaccgcggc
     2761 aaaacttctg gaaggagtga tgacaccaat gaagaagtga tcaggaagcg gtttgcagtg
     2821 tatattaatg agacaacacc ggtagcggag cattacaaaa aagcaaagaa atttatagct
     2881 ataaaaggtg aaggcagtgt aaacgaaatt tttagtgctt tatgtaaatc gatagataag
     2941 aaaatggaca gggattagcc tgcattctta tacttcatct tcacttgccc tttttaataa
     3001 atctttttct tcagctgtca gggagttgta tcctttctgg tttatcttat ccaggatatc
     3061 atcaattcgc tgctgggtta cattcggcgt tcttttataa gggtctgagg aggacttata
     3121 aaataattgt tctttaatac tcttttgtcc ccgccggggc ttatccgggt taaagagatt
     3181 gtttacccaa tcaaagaagt tgctcatcca atcactccaa tcgtatccac gccggatgag
     3241 ccagataaat aaaaagccca taaatgcacc tgctacttgc ggcatatagg cttccacgct
     3301 gtaataagga acggtggcaa ttgaaatgac caggtaaaaa gctgtgagta tccataatgg
     3361 gaagcctccg tttaacatag gaaagatccg gtatcccggg gaaaccatgg tggtagcaac
     3421 agctatagcc gtaatgcttg ctgaagctcc aaaataaaca gcatttgcag gggtctgtgc
     3481 cgcaggtaaa agattaacgg cgataagaaa agcaatagca ccggccaatc cgccatagat
     3541 gaataaggga ataattttcc ggttgcctgt caggtcctgc atgatgtaac caaaagtcca
     3601 gagccagagc atattcgcca gcgtcatcca gattcctaca tgggtgaaca tggcggtgag
     3661 aatggtccat ggccgcgagg caaaattatt catgtcagcc ggaaggacaa cccatttcaa
     3721 tgcttcgtta gaaaagcggg aagcgacgat agcttcctcc cggaataaaa agtaataaaa
     3781 cactttgatg agtgccaggg taataaatac aacaagatta ataatgatca gccttgtcag
     3841 tgtattatca ctttgtccca gcgaaattcg ttgtctgtat tgcttttcgg taaaaggcat
     3901 atctcacttt atcaggttgc aaatgtaggc taagtgttca aaggagagaa gaatacaaag
     3961 atgtctttgt tttgaaaggg atctctttga ccttttatac acttctttaa tacaacgatc
     4021 tgcggttagt tttgttccag tagagaacaa taaaaaagcc ggtaacagcg ccgcccaggt
     4081 gggcaaaatg ggcaatattg tcaccactga tgcgggccac tcccccaaaa aggtcaatgg
     4141 cggctaaccc gagcatggcc catttggctt ttaccgggaa agggatgaac atgatataca
     4201 gctccgtatt gggaaataaa taagcaaagg ccatcatcac tcccattaca gcacccgatg
     4261 cacccagtgc cggggcagcg gctccgaggt gtgattttac agcttcaata tcattttcgt
     4321 taaaggcctg cagcagttgt tcgcagcgga aatattgcat acctaaatgc agggcggctg
     4381 cacctaagcc acaagccagg taaaaaaaca ggaatctctt tccgccccat acattttcaa
     4441 gcactcttcc aaacatccat agtgtaaaca tattaaacag gatatgaaac aacattggag
     4501 gtggtgcatg agaaaacata tgcgttacga tctggtacgg ttgaaattgc tgagaagcat
     4561 cgagggcacc ggactgcaca agcagctcat gcaatttatc cggcatcagc ggccatagca
     4621 taaatcgttc tgtaatggca aattgattag ccagtaagat ctgtgccagg taaaccagca
     4681 cattaatgat gatcagattt ttaacaatgg gcggaaagcc atcaggccgg gtgtatcgaa
     4741 aatcactcat tgggcaaaaa taagttttat tgtatggctt tgtgctggtt gcctgtaatc
     4801 aatagtaaat ctttatttaa aatatagccg gtagttattt tccctttgct atttgtaaag
     4861 gaactgtgga tgaattctca ttgtttttaa ataattttat tactgatgca taaatcaata
     4921 atagttttgt tgttccttat gctttacatg ccgtgccgtg cccaagacac attaacggca
     4981 tttactaagc agctttctta tcgctggcct tcctggcggt tggatggaaa aaagatacat
     5041 ctaagtaaac tgggtttcga gattctcaaa gtggaagcgg ctgtgccctg gtatcaaaaa
     5101 tcaaaaaata atgaaacaaa ggcttacctg gccatgggaa gtgcagcaat cttagccatt
     5161 cttggcagcc ccgaaaccga tataaccagc ccccgttttg gaaaaaataa tctggggtac
     5221 tccatcggat ctttagtagc tggcggagta agtatatttt tctatagccg agccagaaag
     5281 tttctgaaaa aagctgtacg aatttataat gaaaagcgtt taatttatta ggctttcaat
     5341 ttcaactgca ccacattctc gatatgatgt gtatgcggaa acatatcaac cggctgcact
     5401 ctggtcactg catatttttc atccagtaaa ttcaggtccc ttgcttgtgt agccgggtta
     5461 caacttacat atactacaac aggtgcggct atatcgagga tcttttgtac cagtttttca
     5521 tgcataccag cccttggcgg gtccgtaatg attacatcgg gtcgtccgtg tgcagcaaag
     5581 aactcatcgt tgcaaatatc aatcacatca ccggcgaaaa aagaagttga gtccagctta
     5641 ttcaacattg cattttcttt agcatcttca atagctgctt caatcatctc cacaccaatg
     5701 atcttttttg ctttttcgct tacaaaaatg ccaatgctgc cggtgccgca gtacaggtcg
     5761 tatactgttt catggcctgt cagttcagca aaatccctgg taaccttata tagttgttca
     5821 gcctgtttgg tatttgtctg aaagaaagat ttaggcccga tcttgaactg aaaatcttca
     5881 agcttttcta tcacatagcc tttgccatgc caggtaactg gctccaggtc atataaactg
     5941 tcgttccatt ttgtattgat ggtgtacaat aaagttgtaa tagcagggaa tagctccatt
     6001 acatattcca tcagcttgtt gatcttttct tcatcatttt ctgccactac aatattcacc
     6061 atcagttccc cggtggtaca aagcctcacc tgcatggttc gtaaaaagcc aacatgattc
     6121 cggatatcat aaaaaggcca gccattgctt ttggcaaatg ctttaacggc cagtcgtatt
     6181 gcattggttg gttctgcctg cagataacat tgttcaatat ccaccacttt atcaaaaaaa
     6241 cctttggcat gaaaaccggc tacatcctgc tgcccgttaa tggtttcgtc agcaatctct
     6301 tctttcaata aaaatcgctt attaccaaat gtgtactcga tcttattgcg gtagtatttt
     6361 gtctctgctc cgccaatgat tggttgaatg gggggcaggg gtattttacc tatccgttgc
     6421 aacacatcgc ctacctgttt gcttttatat tgcaactgct tttcatacgg tagcatctgc
     6481 cactggcagc caccgcatac gccaaaatgg gagcaaaagg gagtaactct ttcaccggaa
     6541 taggaatgga attgcaaggg aactccttct gcccagtctt ttttattttt caacaggcgt
     6601 acatccacta catcacccgg caccacattc tctataaaga tcactttgcc gtctacccgg
     6661 gacaggcatt tgccttcagc agcgtagtcc tccaccagga ttttctccag tactatgttt
     6721 ttcttttttt ttctcacgcc gcaaatatac cttaacccgg cctaccggca ggcaggctat
     6781 atggcgggat tatccctgca ctatttcctt acgtgtaaag caacggtttt cattgccaac
     6841 acttctttaa cagagttttc ctgtcagggc ttcaatggct ggagcttttt aaataacttt
     6901 acgggattca ttctgactta tgaaacgatt tacattttta ctgtttttac tatcatcact
     6961 tgcggcaact gcccagaccg gttacgatat taaggttacg ttcaaacctt ttaagaacca
     7021 atacatctac ctgggtcatt attttggcaa gacttatccc attattgatt ctgccaaact
     7081 ggataataac agtgtggcca tttttaaagg gaataaaaaa ttacctggtg gcatttacct
     7141 ggtgggttat cccaataagg caggtttttt tgagatactg gttgataaac aacaacagtt
     7201 ctccattaaa gcagatacag ctactttgtc aaaaggggca cagtttgaaa attcacctga
     7261 caatgtcctg tttgtcaatt accagcaata tatgtcggaa aaaggaaaga aaattgcagc
     7321 ccttcaacaa caactgaaga cggcagccgg taaaacagac tcaactaaaa taacagatga
     7381 attagccaac gaggataaag ccgtaaccac ttacagggag gatattataa agaagaatcc
     7441 cgcagccatg ctcagcacat tattggttac aatgcgggaa cctgaactta ccggggcctt
     7501 aaagaatccg gccaataaag aagattcggt attagcttat aatttttata aaagccatta
     7561 ttgggacggc gtgaacttct gggatgggcg attggcatac actccttttt ttgaagataa
     7621 gatcgataaa tatttttcgc agctggttgt tcccaatcct gattcagtga tcaaagaaat
     7681 tgattggatg ctgggttatg ccagtatcaa cgaagaaatg aaccgtttcc tcctgataaa
     7741 atttgtaaac cgctacctga atcagaaata catgtgggaa gatgccgtct ttgttcattt
     7801 gtttgaaaaa tatttttcgc aaaaaacata ttcctggctg aatgaaacag gtaaaaaaac
     7861 aattacagac agggcttata gtttgatggc caatatcctt ggcacaccag cctctgatgt
     7921 tgaattacct gatccgaatg gtacacccgt ttccctgtat gccctgcagg ctgagtttac
     7981 tattgtagct ttctgggacc ctacttgtgg tcattgtaaa gaagtgctgc caaaactgga
     8041 ttctttttac agagctaagt ggaaagccga cgggttgaag atgtatgcag tggccaaaga
     8101 aacggatggc actaaaaaag actggctcaa ttttatcagt gataataaat tgcaggaatg
     8161 gagtcatgtt tattattcca aggaagcaga taagtccagg acagacaatg gtattcccgg
     8221 ctattcacag ctatacgatg tacagtcgtt tcctacttta tatttgctgg acaaagaaaa
     8281 acggatcatt gcaaaaaaac tgacctacca gcaactggat gaaatagtac aattaaagaa
     8341 gaaaggacaa tagcaacgct taaaatcaaa tcctttatga acagaaaatt cattttcctg
     8401 atcattgcct gcggcaatat gattgccgct tcagctcaaa ctttgttcac ctatggcaac
     8461 tatgccgccg atgccaaaga ttttctcagg gcctataata aaaacaatag tgccgctgta
     8521 acaaacaagg caaaatctat cagcgattat cttgacttgt atatcaaatc aaaattaaaa
     8581 gtgcgggaag cgtatgacag ggggtatgat acactgccaa atattagaat ggaagtggag
     8641 aatctccggg cgcagattgc tgagaattac atgacagatc ccgagatcat gaaccgcctg
     8701 gaaaaggagg cttttcagcg tagccagaag gatatacgga ccgcccatat ttttatcgct
     8761 ttcaaaaatg gttcaggatt tgttgataca ttggctgccc agaagaaaag agatgagatt
     8821 atccaaaaac ttcagaaagg ggaggatttt ttattgttgg ccaaacaatt ttctgatgac
     8881 cccaatgcta aattgaataa tggagaactg gagtatatta ctgtttttac acttccgtat
     8941 gagtttgaga atgccgttta caacacccct gcaggtaagt actcatcacc agttcggtcc
     9001 aaaattggtt accatatttt caagaatctt ggtgaaagaa aatcagctgg taaaatgaaa
     9061 gcccagcaga tattgctggc tactcctccc ggggctgatg aagcaacgaa aaagcagctg
     9121 gccaatcttg ctgattccct gtataaaaga ttgctggcag gggatgaatt tggtcagctg
     9181 gccaaatcat tcagcaatga ttatatcagt gctgctaata atggtattat gccggatata
     9241 ggggtgggcc agtatgatgc ggtttttgaa aaagtagtat ggtctttggc gaaggatggt
     9301 gcttttagta aaccatttca aacagcacat ggatggcata ttataaaaag gatagcagtt
     9361 aaacctactg ttactgatcc gggcaacaaa gatttcaaac aggaactgca acaaaaaata
     9421 atggccgatt cccgctggag agcttcaaaa gattttgtgt ataagcaagt gacagagaaa
     9481 gccggggtca aaaaattcat gtatgatgat gcagcacttt gggccctgtc agacagcttg
     9541 ctcgacagga ggcccatgag ggagatcgga cgtgccatta catccgccac tcccttgttc
     9601 tccattggca aagaagttta tgatgcggct gcatggatca attatgcagg tacttatcgt
     9661 ttcaggcagg atggcacagg tgccaagcca catgaacagg ttagagagga gtggatgcaa
     9721 tatgccatgt ttaattatta caaaaaacac ctggaagatt ttaatgagga atttcgcagc
     9781 cagatggctg agttcaaaga tggaaatctt ttctttgaag ttatgcagca ggaagtgtgg
     9841 aataaggcac agactgatac ggttgcgttg ctggctttgt ataataaaaa taaaaaggag
     9901 tatacctgga aacaaagtgc agatgtggtt attttcttct gctcagacca ggcgatagct
     9961 aaaactgttt acgataaggt aaaagccaat cctgcagagt ggagaaaagt ggtggacatg
    10021 tattctgaga aagtgctggc tgattcagcc cggtatgaat ggagccagat acccaacctc
    10081 aataaaatgt tacccagggc aggtatgata acgacacccc tgttaaatgc tacagataat
    10141 acggcctcct ttgcttatat cgttaacgca taccctcaaa cctctcaacg cagttttaat
    10201 gaagcaaaag ggttggtgat aaatgattac caggctttac tggagaaaga gtgggatgag
    10261 gcgttgcgta agaagtaccc ggtcgtgatc gatcaaaaag tgttgagtga tatttcaaaa
    10321 taaagtcccg aaccataaaa taccgaaagt ccgggaaccc attattaatg aacaatgatc
    10381 tcccggactt tctggttact ggtctttatt gacttgatct ataactcttt taccacattt
    10441 gccactaatt gtggtcttcc taatgtgtaa taatgtaaaa cagggacacc tgcctttttc
    10501 aattctttgg actgcatcag caaccattcg gcacctacat gggccacttc ttcatctgtt
    10561 ttacaacgaa gaacttcact agaaaggtca gttggcaaat cgatatgaaa tgttttaggc
    10621 agtacagtaa gttgtttttt agagtaaata ggttttaatc cgggtatgat cggaacggtg
    10681 ataccagcat cccggcagga tttcacaaat gcatgaaact tactgttatc aaaaaacatt
    10741 tgtgtaatga tataatcagc tcccaggtca attttttgtt tcaggtattg caggtcggtc
    10801 tccatattcg gcgcttcaaa atgcttttca ggatagccgg ccacaccaat acaaaattca
    10861 gttttatgtg ttcctttcag ctcatcttcc aggtaaatgc cggcgttaag attaacgacc
    10921 tgtttcagca ggtcgctggc atatttatgg ccaccgggtt ccggttcaaa agcagtttcg
    10981 tttttcgctg catcacctct cagtaccagt acattatcaa tgccgagata ccgaaggttg
    11041 atcagggcat cttccgtatc attgatactg aatccgccac agataagatg cggtacggta
    11101 tccacattgt atttattcat aatagcagcg cagatacttt ctgttcccgg ccttttccgg
    11161 atcaccactt tctcaaagga gccatccact ctcttcttaa atacatgttc actgcggtga
    11221 taggtcacat tgataaaaga gggtttgaac tccattaaag gatcaagatg cttatacagg
    11281 gcttctatcc ctttaccttt caggggaggt aaaacttcaa aggaaataag ggtgtctttg
    11341 gcctgggcta tatggtcggt tactttcatt gggttgcaaa actaatacag cggtgccata
    11401 tagtaatgaa atcttaaaat agagttgaat cattgcccca agccttattt ttgccttatg
    11461 gccattacca ttgaaaccaa agatcttgta aagaaatatg gcagccgtac ggttgtcgac
    11521 catgtttctt ttgaagtaaa acagggagag attgttggtt tgctgggccc caatggcgcc
    11581 ggtaaaacaa cttcctttta ccaggtggta gggctaatca agccggatga aggcaaggtt
    11641 tttttgaatg accaggatat tacgtcatta cccatgtaca aaagggccaa aatgggtatt
    11701 ggctacctgc cacaggaagc atctgtattc cgcaagctga gtgtggaaga taatattgcc
    11761 gccatcctgg aaatgaccaa tctgcccaaa caagtacaaa gagataagtt agaatccctg
    11821 ctcaatgagt tccggttgca gcatgtccgc aagagcaatg gcgatgtgct gagtggtggc
    11881 gaaagaagga gaaccgagat tgcaagggca ttagcggtag atccgaaatt catattactg
    11941 gatgaaccat ttgccggtat tgaccccata gcggtggaag atatacaggc catcgttgcc
    12001 aaactgaaat acaaaaacat tggcatactc atcactgacc acaacgttac tgaaaccttg
    12061 tccatctgtg acagggccta tttactgata gagggaaaga tattcaaaca tggtaccgca
    12121 gaagaattgg cggatgatga gcaggtgcga aaattgtacc tcggaagaaa ttttgagttg
    12181 aagagaaaag attggttaca tgaagaagct tttaattcga tcaataaact acaagaccaa
    12241 taaattatta aataagccga gggctccaaa aaattgccag gcttactttc ctgaatattt
    12301 ctttttactt tgttgtttat ttttttgact acgcactgtg cattatggat tatcaaaaac
    12361 ttccaaagat tgaattgcat ctgcacctgg attgcagttt aagctacaaa gtggtgcagc
    12421 agcttgatcc tgccattact tatgaagaat accaacaatc ttttattgct cctcccaggt
    12481 gtacagacct tgctgattat attacccgtg ctgtgaaggg ctatgagctg atgcagacga
    12541 aagagcaatt gcggctggtt acattagatc tttttgatca gctgaaagca gataatgtga
    12601 tctatgcaga aataaggttt gccccgctgt tgcacattgc caaaggactt actcctgctg
    12661 aagtcgtaca aaccgtaaat gatgcggtta cagaaggtat tcggcttacc ggtgtgcagg
    12721 ctggtatcat tttatgtaca ctcaggcatt attatgaaga gcagagtatg gaaactgttg
    12781 agttggtaaa acaatttaaa ggaactcatg tagtggggtt tgatatagca ggagatgaag
    12841 caggatttcc tattgatagt catgtacagg cttttcgtta tgcgatggat aatgatattc
    12901 cctgcacagc acatgccggt gaagccaaag ggcctgaaag tgtatgggaa acgttacaac
    12961 attttcatcc ttcgagaatc gggcatggag taaggagtgc agaagatgaa aaattgcttg
    13021 attttctgaa aaataaaaat atacacctgg aagtttgccc taccagtaat atccagacga
    13081 atatttatga gaacatcgaa ctgcatgcag cagataagat ttaccggcat ggtgtatcta
    13141 tgagtatcaa tactgatgca agaacagtaa cccccaccac tttatcccgt gaatattcaa
    13201 tattggagga aacatttaac tggaacaagg aacactattt aaagtgtagt atgcaagctg
    13261 tggcacattg tttcgccagc aaccgcttaa aagaagagtt gcgcattaag ttcagaactg
    13321 cctacgaact actttaaaac ttacattagc ggaacagttc atatttttag gggcattgct
    13381 tcaaattatc tattttgcct gcaacaatga aactgctcag ccctgccata tcatcccttg
    13441 cccgtatgcg gctctggcgt atcgaagcct ggaaaaataa tccgttagat gcacagcggg
    13501 aagtgctgca ggaactggta acttctgccc aatacacaga gtttggccga aaatataatt
    13561 tttcagaact gtttaatgta aaagcattca aagaggccgt tcccattcat gagtatgatg
    13621 atttaaagcc ttatatcgaa cgcatcatgc agggtgaaca gaatatttta tggaatacac
    13681 ccatttattg gtttgccaag agcagcggca ctaccagtga caaaagcaaa ttcattccca
    13741 ttagtgagga aagcctgcag gacgggcatt ataaggcttc aaaagatgtg ttgtcattgt
    13801 attaccagtt ttatcccgag tcagaattgc tcaccggaaa agccttggta ttaggaggaa
    13861 gtcataatat taatccattg aacaatgatg cacagtacgg cgaccttagt gctgtgttat
    13921 tgcaaaactc acccttctgg ggacactggc tgcgtacacc cgacctcagt attgcactga
    13981 tggatgaatg ggaaagtaag attgaaaaat tggcggaaaa cacaataaag gaaaatgtga
    14041 catctatttc cggagtgcct acatggacat tggtattgtt taaaagaatc ttggaaataa
    14101 ccggtaaaaa aacgatgagt gaggtatggc cttcccttga attgtatatg catggcggtg
    14161 tttcttttac gccgtataaa gaacaatttc aaaagctgat aggaaagaac atcaattacc
    14221 tggaaatgta taatgccagt gaaggatttt ttgcagcgca ggatattccg ggacaagatg
    14281 ggatgttgct gtttaccgac catggcatat ttatggagtt catgccggta aatgaatatg
    14341 gcaagacaaa gccgcaaaca gtgggtttac aggatgtgga gattggcaga aattatgccc
    14401 tgatcatcag taccaatgga ggattatgga gataccttgt tggggataca attcaattta
    14461 tatcattgag gccttaccgg gtaaaagtat cgggtcgttt aaagcattat atcaatgcat
    14521 ttggcgaaga agtcattgtg gataatacag ataaagcaat tgctgttgct tcagcaaaaa
    14581 ccggggttgt agtgaatgat tataccgctg ccccggttta tttttccgac aacgccaatg
    14641 gtgcacatga gtggctgatt gaatttgaaa aagaaccgga aaacctggct gtctttacac
    14701 aggaattaga tgctgcgttg aaggcgatta atagtgatta cgaagcaaaa aggtataaaa
    14761 gtatcgcttt ggggttgcct gttgttcatt ctctccgcaa aggggtcttt gcagaatggt
    14821 tacgctgtaa aggaaaactg ggagggcaac ataaagtgcc ccggttgagc aatgagcgga
    14881 aatacctgga agaaatttta caggtgaata caattacatt tgacaaccaa taaaatcagt
    14941 attatgaaat tactcgaagg aaaagtagca attgtaacag gtgccgcccg tggtattggt
    15001 gaaggcattg ctttaaaatt tgcagcgcag ggagctaatg tggcgtttac atatgtaagt
    15061 gacagcagtg cagaaaaggc agcagccctg gaagcaaaac tgacagcaat gggagtaagg
    15121 gccaaagcat ataaaagcaa tgccggtgat tttgcacaat gcgaagcttt tgtaaacgat
    15181 gtactgaaag aattcggcac tgtagatatt tgtgtgaata atgccggtat ttcaaaagat
    15241 aatcttttac tccggatgac accagagcaa tggaatgatg tgatacaggt gaacctgaac
    15301 agtgtgtttt atatgaccaa gcaggttatc cgcccgatga tgaaagccaa atcaggttcc
    15361 attatcaata tgagctccat tattggcgag attggtaatg cagggcaaag cagttatgca
    15421 gccagcaaag ccggtattat cggttttact aaatcagtag ctaaagaact cggcagcaga
    15481 aatatccgtt gtaatgccat tgctcctggt tttgtggaaa cagatatgac cagttatttg
    15541 aaagaaggag aggctgctga caaatataaa gccggtatcc cattgggaaa atttgctact
    15601 gctgaggata ttgcgaatgc aacattgttc ttagcctctg atatgtcttc ttatatcacc
    15661 gggcaaacat tgagtgtttg cggaggatta aacatctgat ataatttgaa aatagaaatt
    15721 gtaaaagggg atattaccaa aacagcagtt gatgcgattg ttaatgccgc caatagttcc
    15781 ttgttggggg gaggaggtgt agatggggct attcacagag ctggcggacc tgccatttta
    15841 gaagactgca gaaaaatcat cgccaaacag ggaggatgta aaacagggga agctgttatt
    15901 acaacagcgg gtaagcttcc ttcaaaatat gtgatccata cggttggccc ggtttggaac
    15961 ggaggccaga acgatgaaaa taaaaagctt gcttcctgtt attttaattc cctttcactt
    16021 gctgttgagc atggttgtaa gtcaatagct tttcccaata taagtaccgg gatatatggt
    16081 ttccctaaag aaaaagcggc agttattgca atcgaaacaa taacaacttt tttaaacagt
    16141 aataatttta tcggaaaagt tttacttatc tgttttgatg atgaaaatta tacgcttgca
    16201 aaaagagagc ttgaaagact cagtcccgga gtttgatgtt tctcttttta atgtaagcat
    16261 gccataaaat catagctgcg atcgttcggt aaggccgcca tggtgctgct attttcagca
    16321 tttcttcttt tgatatatgc ggatgcaatt gttttatctc tttcaagcta ttaaccaaag
    16381 caatatcgcc taaaggaaac aggtcagttc gttgcagggc atgcatcagg tatacatcaa
    16441 tcgtccagtg cccaattcct ttaagtttaa tcagctctga ccttacctct tcatctggta
    16501 gtgcccttaa tttttttaat tgaactttct tcaattgaac cgcttcagcc agtcctcttg
    16561 cgtagcccat tttttgccgg gtgaaatagc aggctctcat ttcttcatca gttagctgaa
    16621 gaattttggc aggtgttaca tacccgattt tttgtttcag tttcttaaag gcagcataag
    16681 cagctgccag cgaaacctgt tgttctagga tggtaagtat taaggtttga aatgtattag
    16741 gtcgggtcca caaaggagga taaccatgtt gtgtaattat tccttttagc tccctgtctt
    16801 tccgggtcag tttatcgcag aggaggtgaa aattagccga atcaaatgtt tgcactattt
    16861 cttcttgaat gtttcgatcg catttttggt aaaatcactt aataccagtt ttccgcttat
    16921 cgcagctctt tcaacaagca gcttgtccca atgttctgtt cctttccaga atattttttt
    16981 catttcggcc attgcttcgg ggctggagtg aactaataaa taaccaagac gactgatggc
    17041 ttcatccata tcttccacgg ttgcgtacag ttctgcaaac aatccccgtt ttctggccca
    17101 ttctgcactg cgccagcggg tggcgtcaat ggccagttgg gaataagctg acatgccgat
    17161 ttttctttcc acggctggtc ccactacgaa cggaccgata cctaccgcca gttcactgag
    17221 ttttacttct gctgcttctg tggctatcgc ataatcaaca gcggctgcta tgcccactcc
    17281 gccacccaca catcttccgt gtatccgccc gatgatcagt ttgggacaaa tgcgcatcgc
    17341 atttattaca tgggcgaagc cggtaaagaa tttcaatcct tcctctttat tcctgattgc
    17401 cgacagttca tcaaaagatg caccggcaca aaatgctttt tccccggcac tgcgaagaat
    17461 gataagcttt gtttcttcct cgttaccggc agcatggata gcctgtgcca gttcttccaa
    17521 tatttttccg ggcagtgaat tgctttgcgg atgaaagaat tcaattatcc tgatgccctt
    17581 gtgcagttca cttcttacgt atgctgtagt cataacttaa agtttagtct tttattgaat
    17641 cttttttgcc accatggtca ggatcgttgc caggccagtc acttcattga cttcatcgat
    17701 catttccacc agccatttta cgatcccttt atctatgtca tcacctcttt tgaaatcttc
    17761 tggtttctca attactcttt tatcgttggg tagtttttcc ttacaggtaa aacggatatg
    17821 aatttcagca ccgggataaa ccggcttggt aaaacgtaat tcatcaatgc cataatttaa
    17881 caataccggg ttcttttcat agctatcaac aaataatcct gcagcaatgc tcatgataaa
    17941 atagccgtgt gccacctgtt gctcaaacat agtgcctgta aaatcagttg ttttaatatg
    18001 cgcataaaaa tgatcaccac tcaaatcagc aaaacgatca atatcttctg aagtgatcac
    18061 tcttttttct gtaatgatct ggtcgccgat ctgtagttcc tcgaaatatt tttggaaagg
    18121 atgtttgccc gggtctttgc cattagcatg aggctggtat acattggtga tcgctgtgat
    18181 ggttgtggga gaaccttgca aagcggtgcg ttgcaaataa tgctttaccc cgcgaaggcc
    18241 gcccatttct tcgccgccac cggcacggcc ggggccacca tgcaccagca aaggcaaggg
    18301 tgaaccatgt cctgtacttt cttttgcaca ttcattattc agcaccagga tccttccgtg
    18361 atgggaggcg gcccccacta catattgttt tgcttctttg taatcagcag tgacaatagt
    18421 ggagacaagg cttcctttgc ccagcttgct tagtacaatg gcatcatcca tatttttata
    18481 cggcatgatg gtgcttaccg ggccaaatgc ttctatatta tgcggctcgt aagatgaatg
    18541 aggtttttca tttagtaata ataccggcga catgaaagcg cctttattgg catcggcatc
    18601 tattacttcc acactgtcta atgaaccata tacgatttga gaagaggcca ataatttttg
    18661 tacctgttcc tttacttcat tacgctgggt ttgcccggca aggcttccca tccttacttt
    18721 ttcattgagc ggattaccaa ttgcggtttg tgataatgag gtgccgatcg ctttccacac
    18781 gtcttccatt ttgttttcgg gaacaaatat cctgcggatg ccggtacagc gctgcccggc
    18841 tttaagggtc atttcctttc gtatctcttt tataaagata tcccactccg gtttatcggg
    18901 agttacatcc gcacccagca caatacaatt gagactatct gcttccatat tgaaaggaac
    18961 attgttgtct agtatggcag gcgttttttt cagcatggaa ccagtagaag cacttccggt
    19021 aaatgttatg atgtcttgag aagtgacgtg gtttaaaaga tctccggcac taccacatat
    19081 tagttgtaat gcgccttccg gtaaaatttt tgaagcaatg atctctttga caaatgcttc
    19141 cgttaaataa gaagtaacgg tggccggttt tacaatggca ggcacaccag ccagcaggtt
    19201 cactgcaatt ttttccagca ttccccacac aggaaaatta aaagcattga tatgtatggc
    19261 cactccttct ttcggaacaa gtatatgtgt tcccatgaag gtattgttct tgctcaggtt
    19321 gtgactttca ccgtcaatac aaaatacttc atccggaaat ttccgtcgca atgatgcatt
    19381 ggcaaataaa ttgccgattc ctccttcaat atctacccag ctatctgctt tagtggcacc
    19441 tgtctggtaa ctgattgtat aaaatttctc caggtgattc ctgaggtgca gtgctaaagc
    19501 tttcagcata ttaccccgct ggtgaaacgt catctttcgt aacgcagggt tacctgtagt
    19561 tctgccatat tcaataatac ttttgaaatc cagtcctttg gtgctggcag cagcaacagg
    19621 agcaccggtt acggcattat aaagtaattg cccttccccg tcacccgtta tccagtttcc
    19681 ggtaatatag ttctccagtt tcgtcatata tgcaagcatt atataagcga aattacctga
    19741 attgtaaaaa ccattaaaga atgattttta tcatacatat tggaatccac tgagtttacc
    19801 tttgctgttc aaaaaaacct gtatatgaaa aaaattgttt tgttaatcat ggcatttgca
    19861 acgttggtta ttgctaatcc tgtacaagca caggaaaaag ccaaatggaa cgaaaaagag
    19921 gcttttcatg agataatgag taaaactttt catccggctg aagaaggaaa gttggaaccg
    19981 ataaaaagcc gcagtgctga aatggttgac atagcaacag cctggaaaaa ctcaactgca
    20041 ccggaagggt ataaccaaaa ggcaatacaa aagaacctga aaagcctggt gaaagatgcc
    20101 aaaaaattaa ataaactggt aaagaaaaac gctgctgatg ctgatattaa agcacaatta
    20161 gtagaacttc acaccctctt tcatgagata acagaaaaat gtgaacatta aaaaagaatg
    20221 gtatgctaaa aaaaatcttg attgctttcc tggctatggt cattatcaca gcctgtaata
    20281 acgaaaaatc cggggaagac catacagggc atggccccca aaaagaagcc ccaaagaatg
    20341 ctgctgacag tctttataat gatgtaatgg ccggtcatga tgaagtgatg ccaaaaatgg
    20401 gaaaaataag aggggcgcaa aaagaagcac aactaatgct tgactctatt gccaaactac
    20461 cggcaaaggc acaaactgcg gcagctgcat taaaagcaaa actggagtcg cttatcaatg
    20521 acctgaacta tgcagatttt gccatggata aatggatggt ggaattcaac atggattctg
    20581 caaaagataa tatagaacaa agggtgaaat acctggcgga agaaaaactg aaggtgacca
    20641 aagtaaaaga ggcagtcctg ggtggactgg ccaaagcaga ttctttgctt aaggccaaag
    20701 ggatcaaata gtttctggtt ccaccccgaa ttgtcgcagg tggtgtagtg catgtttgaa
    20761 aagtaactgc acattttgct caaaattcag gtcgccgaaa aaaggattcc gggttattaa
    20821 tgcggggttc ttttcaaaag cttcaaaaaa ataaatcaat tccagttgca gggcgccgat
    20881 ggcgccttgc actgttttat gcactaatgg ggcaggctct tcacccatca gcgggttttt
    20941 ggtattttct ttaaaaggtt tgtcgctcat cataaactcc cgcatccggt caagattttc
    21001 ggggggagtg atgatggtat caattttcag cctgccgctg gcattacgaa ccgcatcacc
    21061 accataatgt tctatcattt gctgcacatt cattttgccc cattttggag atgtggatgg
    21121 atccagccgt tggagcaacg ggaccaattt ggtgcgaaga aaattttctt tctctaaact
    21181 catctgcaat acattcaatc aggcaattta caaaagattt aaatatttaa cggcaggtat
    21241 ttattcagaa tgtatagtat gtgttaggtt aatttgttac taaagttgct gtatattctt
    21301 cagtcagaac tattgttgat aaataatatg gcctgatgat ttattcatca ggtcattttg
    21361 caaaatacat tttgtagaca gaagtaaatt taattgttaa tacaataaaa aattcaccgt
    21421 tagttttcct tttgtggctt tatacgcctg atagtttatt gtgattattt ttttatacca
    21481 gtctgtatgc cagaaagtat caacattgcc gcctttaatt ttgaatcctg atatgataaa
    21541 ggtatcatca gcgctacctt taatatctac tttcaatgtc gttgggtaag ttttatcggt
    21601 tttgatatta attattgtgt ctttgcccaa ttcatttatg attatatgct ttatttcagc
    21661 tctctgtctg catgaaatgc ttacagctac taatgctatt attgtaaaaa aaaattcaat
    21721 tttcataata gatttttaat ctgttggaac taattgagaa tttggaagta tttctgattt
    21781 aaagcccaat tggatgacaa aatagttgat tgacaagttg caaacaataa cacaaactgg
    21841 taatttttcg agaaccgatt tattaggctt gattgcaact ttggcccatg gctcaccggt
    21901 tttttcttaa attgcagcaa tgtccagcaa gctccgactt ttcaccaagc ggttttttat
    21961 ttattccaat gttatcgttg tattcatttt cattttatcc tgcctggtgc cttatctcaa
    22021 tccgcagaca tggtggttca tctccttttt cggactggcg tttccttttt tacttttact
    22081 ggttatcttt tttttactgg gctggttaat gatcttaaaa ccacgactgg cactgatttc
    22141 ttttgtagcc ctgctggtgg gtataaaaag tatcgctgtt ttttttgcct ttcataaccc
    22201 ccgggccttt aactatgata aagaacctgg tacgctgcgg gtggttagct ggaatgtagc
    22261 caggtttatt gaattaaagc gaaataccaa taaaggaagt gaaacaaggc ttaagatgtt
    22321 cgagctgctt aaacaacaaa atgcggatgt attatgcctg caggaatttc atacgtccac
    22381 taacccggag tattataata acatagaaaa aatacaaaaa gaactgggct atccttttta
    22441 ttatttttct tttgatgaag atggggacaa tcattattac agctccatca tattttcccg
    22501 gtacccgatc gttgacagtg gccgcatccg ttatccacgg cccactttac ccgacgtttt
    22561 attgcatgcc gatataaaag tgaataagga tacacttcgt ttttatacga cccacctgca
    22621 atccttacag tttggcaaaa gtgattaccg gagtattgaa aagatcaaag cggcagaaga
    22681 cagtcttctg cccagttcca ggagtattct gtcaaaactg aaaagaggga tctccaatcg
    22741 tagtatacag gctgatattg ttaacgaagt gattggtaac agcccgcatc cggtactggt
    22801 ctgtgctgat cttaatgatg tgcccaactc gtacacttat tttacagtta aaggcgatat
    22861 gcaggatgct ttcctgaaga aaggaacggg tatcggaaga actttttccg ggctttcacc
    22921 caccttgcgc atcgatttta tttttgccga taatgctatt aagataaagc agttcagaag
    22981 ggttactaaa aaatattctg atcactatat gctggtggca gatgtagaat tgaaaaaata
    23041 aataagaatg gtatcttcgc tgtcatgaat ttcaaattca cataccgctg ctgttgttgt
    23101 tgcttccggg cctgctgatg cccacggccc tgcatttggg cctcctaaat caaactattt
    23161 tatcatcttt gcggtacttt aatgtaacag gataaactgg ttaccaacaa ctgtaccaca
    23221 gactgttcaa tacaataatt tatgagtcaa ctgaaagtat ataattcact aaccaggcaa
    23281 aaagaaattt ttaaaccgat cactcccggg catgtgggca tgtatgtgtg cggacctacc
    23341 gtaagcggtg aaagccacct ggggcatgca aggccttata ttacgtttga tgtcgtctat
    23401 cgttacctga cctatttggg taataaggtc cggtatgtac gcaacattac agatgccggg
    23461 cattttgaag aagaaggaag agaagcggtt gacaaaatcg ctacgaaagc agtattggaa
    23521 aagctggaac caatggaatt ggtacagaaa tataccaacc tgtttcattg ggcgatggca
    23581 cagttcaata caatctcccc atctattgaa cctactgcaa caggacatat tgtggagcag
    23641 gtagaaatga tcaaacagat cattgaagcc ggctttgggt acgaagtgaa tggctctgtc
    23701 tattttgatg tgaaaaagta tgcagcctca catgattatg gaaaactgag cggacgggtg
    23761 attgatgacc tgctggaaac aacaagggaa ctggaagggc aggaagagaa aagggacagg
    23821 gcagactttg ccttatggaa agcggcagca ccggaacata tcatgcggtg gcaaagtccc
    23881 tggggtgtgg gatatcccgg ctggcatata gaatgttccg ctatggccac aaaatacctg
    23941 ggggcacaat ttgatattca cggtggtgga atggacctgt tgtttccgca ccatgaaagt
    24001 gaaatagcgc aaagtacgat ctgcaatcac acagccccgg ttcgttactg gatccataat
    24061 aatatgatca ccattaatgg ccgaaagatg gggaagagct ataataatgt gattaagctg
    24121 acggagctgt tcagcggaaa tcatcccata ttggaaaaag cgtatcaccc gatgacggtt
    24181 cgttttttca tattgcagac acattaccgg agcacacttg atttcagtaa tgaagcattg
    24241 caggcatcgg agaagggatt gaaacgattg tgggaggcgc atgaaaagct gcaggcaatg
    24301 cattacgagc cacaggaagg aacaacggac cccgaactgg aatcccgtat cccgaaactg
    24361 gtagccgagt tcaatgagtt catggatgat gatttcagca ccgcaaaagt aatggccaac
    24421 atgtttgaga tcgtaccggt tataaattcg atgaaggaca aaacaattcc tgtctccgcc
    24481 atttcaaaac agacctttga attgctgcaa ggcagtttta aaacctggct ggaagatata
    24541 ttcgggttga agtctgtcag cgaagctgat aatgcccgat tgcagggagt gatgcagttg
    24601 ctgatcgata tccgtaagga agcaaaagga aaaaaagatt ttgtcacctc cgataagatc
    24661 agaaatcagc tggcccagtt aggaatcacc
//
LOCUS       DAORTI010000044        24687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1589_length_24687_cov_7.850276, whole genome shotgun sequence.
ACCESSION   DAORTI010000044 DAORTI010000000
VERSION     DAORTI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24687
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1589_length_24687_cov_7.850276"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1034)
                     /locus_tag="PLO70_07650"
     CDS             complement(435..1034)
                     /locus_tag="PLO70_07650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQZ74375.1"
                     /translation="MPTDIFQQQPVLENERVLLRPIQESDFQQLLPFSLNEPEIWKYG
                     LVTAAGEDNLRNYITMAIKNREDKKEYAFIVFDKKANRYAGSTRFYDIQQSWLTTQLG
                     YTWYGKEFQRTGLNRHCKLLLLTYVFEEWGMERLEFRADANNAGSIAAMKAIGCVEEG
                     ILRNHMPTASGGRRNSIILSILKTEWFGGVKERLLQNIR"
     gene            complement(1052..2326)
                     /locus_tag="PLO70_07655"
     CDS             complement(1052..2326)
                     /locus_tag="PLO70_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQZ74376.1"
                     /translation="MLNRSISKWAMVLLIINGVIGSGIFGLPAKVFKETGVYSIAAFL
                     VCAVAVFVIILCFAEVSSRFSKTGGPYLYALSSFGKLPAFLTGWLLLVTRFITYAALI
                     NLLVSYLSVFAAWFTLPEARIIVIVLFTLLLAYINHIGIRNTTRINNFLTISKLLPLL
                     LFIVVGSFFIEPAQYEIKHIPDFSSFSSTVLLLVFAFGGFESVLVNSGEVKNPEKNLP
                     FALLLAAVFITTIYILIQVVSIGTLPALASSDKPLAEAAGLFMGKSGASIIAIGAIFS
                     VAGTLNAIMLVGSRLPYAFSEEKQFPGLFSFIHPKYKTPTWSLLLFMSITIIVSLNYS
                     FLSAASLSAITRVMIYGIVCTTLILLRKKNPDQANFYKLKHGNIIAVAGILITLWLLS
                     SSRLRELRDVGIAIMAGLVIYILVNRSKKNNH"
     gene            complement(2376..3686)
                     /gene="murA"
                     /locus_tag="PLO70_07660"
     CDS             complement(2376..3686)
                     /gene="murA"
                     /locus_tag="PLO70_07660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQZ74377.1"
                     /translation="MHSFEVRGNKKLQGEIIPQGAKNEALQIISAVLLTPEKITITNI
                     PDIIDVNLLIELLGDMNVNIERTSRDTCTFRANDVNIDYLHSDAFKKKSGKLRGSVML
                     AGPMLARFRKAFIPKPGGDKIGRRRLDTHIVGFEKLGASFIYHEEDGFFHLEAPQLQG
                     AYLLLDEPSVTGTANIIMAASMANGTTTIYNAACEPYIQQLCKMLNRMGAKISGIGSN
                     KLTIEGVSYLGGTEHSMLPDMIEVGSFIGLAAMTQSDITIKGAGIDHLGIIPEKFQQL
                     GIQLEFNGDDIHIPSQESYEIQTFLDGSVMTIYDHPWPGFTPDLLSIVLVTAIQAKGS
                     VLIHQKMFESRLFFVDKLIDMGARIVLCDPHRAVVIGLEREQMLRGITMSSPDIRAGV
                     ALLIAALSAEGKSVIQNIEQIDRGYQDIDGRLRKLGADIKRTGS"
     gene            complement(3843..4553)
                     /locus_tag="PLO70_07665"
     CDS             complement(3843..4553)
                     /locus_tag="PLO70_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HQZ74378.1"
                     /translation="MDIDSMEYNTTRNYLTMREYGRHVQKMVEHTMTIEDPERRQRNA
                     QAVIELMGFLNPHLKNVEDFRHKLWDHLFLISDFKMDVKSPYPIPTRETLKAKPDPLP
                     YPKRHPKFSHLGKNLEVIIDKALQEENPEKKLGFANAVAYYMKLAYNNWHKEIVHDDA
                     IQSELSSITNGQLTFTNTPFVKAFRPQQEGRDNRGGYGKRPGGKFQQRGGGGGGQRRN
                     DGRDNRGGGGGGFKKKRY"
     gene            complement(4667..5869)
                     /locus_tag="PLO70_07670"
     CDS             complement(4667..5869)
                     /locus_tag="PLO70_07670"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HQZ74379.1"
                     /translation="MKNSYSKFFIAVGTFILMNLPGFNTANAQCPYGFPSGTTLYDTT
                     INTPPGINTLQVKVPQADPMAGMVTCLRICVSITGVVDSVSVENNSASPQTADIYYIR
                     TDQISGPGLSSPLNNSINHHYGPYGLGPTNGTLGSGPDFISISKDTLLNAVQVCQTIT
                     NLDSLFQFYGHDSITYNYNITAFTNVTCTGGNYNSTVATSAIVRFRFEYCTCPGYVLP
                     LHLRNFAINKIADNSVKLNWSGFNDPSASYHYEAEMSRDGRQFSTVGTVAKNASSNDI
                     YDFIYTTRQNESGEFFFRVKQVYANGYTRFSDVKQVSLKNSSLPKFIVTPNPSNGIVG
                     IKFDNNEGGKMMLEIYNSQGQKVMQKEIVVSGTSYHQVATLQGGVYWLRLTDVTSQLS
                     SVNQLFIK"
     gene            6082..7272
                     /locus_tag="PLO70_07675"
     CDS             6082..7272
                     /locus_tag="PLO70_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQZ74380.1"
                     /translation="MQQCWCLFTLKSDGQQLPLLLLMRIAVNTRFLLKDYLEGYGNFL
                     YETFRRITENHPEHEFIFIFDRPFDIRFVFKANVTPVVAGPAARHPLLWKYWYDIKIP
                     ALLKKYKADIFVSCDGFCSLNTKVPQCLVVHDLAFIHFPSGIKKSHLLFYKRFTPKFL
                     ATAIAIATVSQFSKNDIVRQYNTAAGKIDVVYNGVKEIFRPLSYENKEAVKKKYTEGK
                     EYFVYAGAIHPRKNLMNLLKAFSVFKKRQQTGMKLVLTGRLAWKYESFTASLKTYKYR
                     NDVVLTGYVPEEELVSIIGAAYGMVYPSLFEGFGVPVLEAMKCEVPVITSSGSAMQEI
                     AKEAALYADPGNHTDIADKMMLLYKDENLRKEMIQKGKQVIKEYNWDNTAALLWQCIL
                     KAHQ"
     gene            7329..7676
                     /gene="gldC"
                     /locus_tag="PLO70_07680"
     CDS             7329..7676
                     /gene="gldC"
                     /locus_tag="PLO70_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HQZ74381.1"
                     /translation="MNKSTITIDVHTDENRIPASIAWSATDTSIENAQKAKAMMISFW
                     DGAEKAALRIDLWTKDMMVDEMADFFYQTMMTMADTYGRATKYADQVEEMKTFAKGFY
                     EKFREKQLKENKA"
     gene            7762..8214
                     /locus_tag="PLO70_07685"
     CDS             7762..8214
                     /locus_tag="PLO70_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQZ74382.1"
                     /translation="MNLEQKIMADLKAAMLAKDEAALRSLRAIKAAILLAKTAEGAGG
                     ELKEEEETKLLQKMVKQRKDSLEIFQQQNRPELARKEEEEIAVIEKFLPKQLSAEEIK
                     AALTKIIAETGASSPADMGKVMGAATKQLAGKADGKTISALVKELLAQ"
     gene            8283..8825
                     /locus_tag="PLO70_07690"
     CDS             8283..8825
                     /locus_tag="PLO70_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQZ74383.1"
                     /translation="MIIDLILGVIILIAIFKGYQRGLIIGLFSLVSVIIGLAAAMKLS
                     TVVAGYIGEAVNVSDEWLPVISFAIVFIVVVLLIRLGANAIERTVEVVMLGWLNKLGG
                     IIFYVAIYTVVFSVLLFYAEQVNLVRSETIEKSISYSFVQPWGPRVINSVGSIVPLFR
                     DMFAELELFFENVSKEIPVR"
     gene            9037..9813
                     /locus_tag="PLO70_07695"
     CDS             9037..9813
                     /locus_tag="PLO70_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ74384.1"
                     /translation="MDYEIKQQDKYKFVEEGSGEPLVLLHGLFGALSNFQSLIEYFRH
                     HYKVVVPMLPLLEMDILHTSVGGLAKFVNKFLDARKLDAVNLLGNSLGGHVALVHTLK
                     HPERIKSLILTGSSGLFENGMGDSYPKRGDYEYIKKKTELTFYDPNTATKDLVDEVYS
                     IVNNRLKAIKIIALAKSAIRNNLGEELNQIKQPTLLVWGNNDTITPPFVGREFNKLIP
                     NSELHFVDKCGHAPMMEVPEEFNAILHKFLKKLNEPATVA"
     gene            9882..10544
                     /locus_tag="PLO70_07700"
     CDS             9882..10544
                     /locus_tag="PLO70_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQZ74385.1"
                     /translation="MLTRELESQTLPYLHLTDKVYQALQLMNDNHVTHLPIVEADKYI
                     GLISEEDLLQAENDNAELQTLQQSFANPSVKGDEHFLKAVQVAAENGLSVVPVLGDDN
                     ELIGTVVYNDLLKHASEFMSLNEPGGLIVLEMESNQYSFNEISKLVETNNAQITQLNT
                     TNDAETGLMQVTLRINKPEVSDIVATFQRYEYNVKYFFGEELYTNELRSNYDNLMNYL
                     KI"
     gene            10617..12047
                     /locus_tag="PLO70_07705"
     CDS             10617..12047
                     /locus_tag="PLO70_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQZ74386.1"
                     /translation="MREKKHSIWLVAALISCCSITAQQPDMPPPVADSSVAAAARFIV
                     GEIFINGNRKTKASIILREIPFHPGEEYPLQDLVKKFEDARRQLMNTTLFHTVVVAAK
                     SFEGNKIDIVVDVKERWYLFPVPYLKPVDRNLNQWIVEQKASLSRVNYGAKVLYNNAT
                     GRNDKFRLWLIAGYTKQVSISYDRLYIDKAMKWGMKFGLSAGKNRELNYNTINDKQVF
                     LKDNDRYIRNFVNTYAELTYRRAIKTRHSFGIAYSSEQVQDTIVTLNPTYFKSGRNRI
                     KFPELYYSMAYYDVDYIPYPTQGYAANVSISKRGFNNTINVWQLHVKGFGSWHLSPKT
                     FVNLYAYGGIKLPFKQPYFNQRFLGYGDVFMQGYEYYVVDGVAGGFLKATLAREMLNF
                     NIRIPPRKGKEAERIPVRIFGKIYGNSGYVHNPQPGENNLSNRMLNAAGIGIDILTLY
                     DVTFRFEYSFNQLGQNGLYLHRKTIF"
     gene            12050..12928
                     /locus_tag="PLO70_07710"
     CDS             12050..12928
                     /locus_tag="PLO70_07710"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQZ74387.1"
                     /translation="MKAAIYSRVFEEVHKEEVQLFFDELASQKIEPVIFLNFFDQIKN
                     EINLPADTKIFSLSEDLTDEIEFIISLGGDGTLLDTVTLVRDKKISIMGINFGRLGFL
                     ASIGRDEVKTAVKAIARRTYVEDARTLIHVDADMPLFGNVAYALNEFSIHKRDVASMI
                     KIHTYVNGEFLNTYWADGLIVATPTGSTGYSLSCNGPVVFPDSGSFVITPVAPHNLNV
                     RPIVVPDTNIISFEVESRSDQIICALDSRREIVHKNVTLAVRKESFDINLVRLSENNF
                     LQTLRNKLTWGLDKRN"
     gene            12998..13858
                     /locus_tag="PLO70_07715"
     CDS             12998..13858
                     /locus_tag="PLO70_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQZ74388.1"
                     /translation="MRKKFLGIVVFFCVAVFAGRQLHAQNSVVQEGEFGFGVGAGHYF
                     GDLNTKARLNRAKFAATAFFRKNFGNYIAARVGVSFAQLGYSDIYNSYNKQMFSRNLS
                     FNSKVWELTLQGDFNFFRFMPGEPEYSFTPYITFGAGIFSYDPYAYLRGEKVFLRPLG
                     TEGQGSSLYPDRKQYSSMAICIPFGGGIKYSLNERINIAFEVLHRFTNTDYLDDVSKT
                     YVDPAAFPLNPDGSQSNAQLLSDRSYEVGPPIGIPNRQRGNSKQKDQFVTAMFHVTFN
                     LQSYKCPTAN"
     gene            13973..14377
                     /locus_tag="PLO70_07720"
     CDS             13973..14377
                     /locus_tag="PLO70_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74389.1"
                     /translation="MRLTTIILSSIVIAVFSLTTAFKTTDTGSWTFLGDKNVGFGVDH
                     DVIHFGNWKDDVRQVKLKITDGPLKMYRMNIHFDNGSVQEVTLRNRFAQGSESRVIDL
                     DGGLRHLNKIEFWYETKGFLRGKSRVAVWGRN"
     gene            14520..15287
                     /locus_tag="PLO70_07725"
     CDS             14520..15287
                     /locus_tag="PLO70_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQZ74390.1"
                     /translation="MSDLLEMIDRDRLPRHIAIIMDGNGRWAKEKGEDRLFGHFHGVE
                     SVRNIVEGCAELGVEYLTLYAFSTENWDRPEYEVVGLMELLVSTIRKEVESLNKNNIR
                     LHVIGDMSMLPEYAKKELDEALEITKDNKGLNLIMALSYSGRWELLNAVKNIAYEVKQ
                     GRLNVEEINQDTLQHFLCTSGFPDPELMIRTSGEYRISNFLLYQLAYAELYFTNVRWP
                     DFRKDNLYEAILDYQNRERRFGKTSEQLTDANESSKL"
     gene            15452..18193
                     /locus_tag="PLO70_07730"
     CDS             15452..18193
                     /locus_tag="PLO70_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQZ74391.1"
                     /translation="MQRFLQRLGGTLMTAVMFVFTVSAQEVPDTTKPTSIDPKLIEWQ
                     NARIVKEYTIAEVNITGIRYLDTSIVYSIANLQPGDKFVHPGADVFGKAISNLWRQKL
                     FSNVQIYVTRIDGDKVWLEINLQERPRLGSYKFVGIKKSEEEDILGKITLTKQTIITE
                     NTRRELTEKITGHFSEKGYRNVKVKIEESPDPAFVNSNVLTIIVDKGGKVRINEVGFY
                     GNENIEDLRLKKQLKGTKEMSKLTFYPDKATSPYGENKPFSFNQYMRDWGFLSLSKTK
                     MLLDPYFRFKLFSGAKFDAKKYEEDKEKILDYYNSMGYRDAQIVADTQYTTANGNLNV
                     DLKVDEGRRYYFGNINWKGNAKYSDSVLNVILGINKGDIYNVAILNKRLGKEASQDGG
                     DISGLYMDEGYLFFRAEPIETAVYNDTIDYEIRIMEGPQARIKNVTIAGNEKTKDYVI
                     RRELRTIPGELFSRSDLIRSQRELGNLQYFNQETINPGVVPNAEDGTVDINWKLEEKS
                     SDQLELSAGWGGGIGLTGTLGVTFNNFSIRNIWKKSAWDPLPTGDGQKLSLRMQSNGR
                     AYRSYSASFTEPWLGGKKRNSLTIGFNNSKYSNAFDPFTGQIDRARSDSNFLKTTGVS
                     VSLGKQLKWPDDYFSLVYTFNVTQYKLRNYPIFDQNFTNGTSNNVSFKIGLQRSSVFN
                     PIFPTSGSNFLASVQFTPPYSLFNKNIAKSDNKYKNPEYHKWRFNAEWYVPIGKPLGA
                     EKNRQFVLKMAAKYGFMGRYNKDLDYSPFERFQVGDAGLTNNFGLLGYDIIAHRGYPV
                     YQSSDPTVNPDQQSASQFFTIFNKYQLEMRFPLVTNPSSTIYALTFFEAANGWYNYKD
                     YNPFRLRRSVGVGMRFFLPMFGLLGFDYGVGLDRIQPGQSGLKGASRFTFMLGFEPD"
     gene            18322..18831
                     /locus_tag="PLO70_07735"
     CDS             18322..18831
                     /locus_tag="PLO70_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQZ74392.1"
                     /translation="MKKIFLLACMLGFGIVGFSQKYAIIDTRYILDKMPDYKEAQKQL
                     DEIAAGWQKEIDAKQVELDKMYKDYEGEQVMLTEELRKKREDQLFNKEKDLRDQQRKR
                     FGFEGDLFKKRQELIKPIQDKVYNAVQKMAVGRGYDFVLDKSEGITIIFADPKLDKSE
                     DVLKDLGVR"
     gene            18905..19438
                     /locus_tag="PLO70_07740"
     CDS             18905..19438
                     /locus_tag="PLO70_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQZ74393.1"
                     /translation="MKKILSVIAIVGLLSFSVTTNAQTKIGYISVDNVIGLMPEATKI
                     DSLLERYQADTLNPEYASLVQRYQFDDSLYRDSVKTPKAVRDEIAKKLPSYIYQIQNW
                     QQIVNQALEAKQNELLSPLYRKAYDAIKLVAKEKGYTHVMNKEALLVAPDGDDMLKAV
                     AAKLKITIPTNPPPAGK"
     gene            19519..20358
                     /gene="murI"
                     /locus_tag="PLO70_07745"
     CDS             19519..20358
                     /gene="murI"
                     /locus_tag="PLO70_07745"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQZ74394.1"
                     /translation="MQQPGPIGIFDSGYGGLTVLKEIVNKLPQYDYLYLGDSARAPYG
                     NRSFDTVYHYTLQCVNWFFEQGCSLVILACNTASAKALRTIQQNDLPRIAPGKRVLGV
                     IRPTAEIIGNFSETESVGVLATTGTVMSESYPMEIAKFFPEIKVYQEACPMWVPLVEN
                     NEYESDGADYFIKKNLRTIFEKGEDIDVILLACTHYPLLKAKIEEYLPVGVKLLSQGE
                     IVAASLAEYLHRHPEIESRCRKNGQREFYTTDSTADFDNHASTFFGEKLQSRHVDLDA
                     AIS"
     gene            20605..20760
                     /gene="rpmH"
                     /locus_tag="PLO70_07750"
     CDS             20605..20760
                     /gene="rpmH"
                     /locus_tag="PLO70_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQZ74395.1"
                     /translation="MKRTFQPHRKRRKTVHGFRKRMETANGRKVLASRRAKGRKKLTV
                     SDERKLK"
     gene            20833..21213
                     /gene="rnpA"
                     /locus_tag="PLO70_07755"
     CDS             20833..21213
                     /gene="rnpA"
                     /locus_tag="PLO70_07755"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQZ74396.1"
                     /translation="MVKQFTLGKQERLKSRKAIEQLFKDGQRFTITPYRVFYRKAGKP
                     GLQFGAGVSSKNFKKAVDRNRVKRLMREAYRLQKNNLQKHLKDSNTGLNLFFIYTGKE
                     LPSYDIIYEKMALALQKLTDSIEQ"
     gene            21242..21463
                     /gene="yidD"
                     /locus_tag="PLO70_07760"
     CDS             21242..21463
                     /gene="yidD"
                     /locus_tag="PLO70_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQZ74397.1"
                     /translation="MGQLLSLPFIALIKIYQWVISPYLGPKCRFTPTCSHYAAEALQK
                     HGIFKGIWLSIKRISRCHPWGGSGYDPVP"
     gene            complement(21516..22118)
                     /locus_tag="PLO70_07765"
     CDS             complement(21516..22118)
                     /locus_tag="PLO70_07765"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQZ74398.1"
                     /translation="MTFTLAPLPYAHDALEPHIDTLTMQIHHGKHHQAYVDNLNKAVT
                     GTPNENKSLEELVAVAGSISPAVRNNGGGHWNHTFFWESLAANAGGSPSGKLAEAIND
                     TFGSFDAFKEKFATAGMTRFGSGWAWLIVKDGKLEVTSTPNQDNPLMDVAEVKGTPIL
                     GVDVWEHAYYLKYQNKRADYLSAFWNVVNWNKVAERFEKA"
     gene            complement(22230..22526)
                     /locus_tag="PLO70_07770"
     CDS             complement(22230..22526)
                     /locus_tag="PLO70_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74399.1"
                     /translation="MTKATKSSAKHPKSGREMRIDAAIYIPFKDAVLQSLKGSKGKTF
                     TQLTDDVVKIINKKMPGFNKSIPWYTISIRLDLESRGIVETFMEKGKKLNRLKK"
     gene            complement(22530..22967)
                     /locus_tag="PLO70_07775"
     CDS             complement(22530..22967)
                     /locus_tag="PLO70_07775"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQZ74400.1"
                     /translation="MTDEYFMTQALKEAKIAFEDDEIPIGAVVVVNERIISRGHNMTE
                     RLNDPTAHAEMIALTSAFNSLGSKYLPDATLYVTIEPCFMCAGAIYWSKIGKIVYGAD
                     DEKNGYKKTAGANWPFHPKTELIRGVMKDECAQLMKDFFKGKR"
     gene            23011..24387
                     /locus_tag="PLO70_07780"
     CDS             23011..24387
                     /locus_tag="PLO70_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQZ74401.1"
                     /translation="MSADITRLVKMRQYFASGATKTYSFRKEQLKKLKSSIASHEQDI
                     YTALYEDLKKSPEESWVTENGLVIAELNTAINNLQRWMEPDSVGTNLVNLPSGSKIIK
                     EPLGVVLIIGPWNYPFQLLINPLIGAIAAGNCVVLKPSEFAPATDAIMKRIIEEVFPP
                     EYILYVQGDGASVVPGMMNNFTFDHVFYTGSTTVGKIIYKMAAERLVPVTLELGGKSP
                     CVVEADANIRVVARRIAIAKFSNAGQMCVAPDYLLVHHSVKDKVLSALKETIQQFFTG
                     KPEESYNYGKIINEKQFTRICNYMQNGTIAFGGRTNREKLFIEPTLLTDVSLNAPVMN
                     DEIFGPVLPIISFHTMDEAKAVIDRHPDPLAFYIYSSDNKKQQAWLEAVPAGGSCINN
                     CSWHLTNHNLPFGGRGHSGLGFYHGKYSFETFSHKKAVMKTPTWLDPDIKYPPFKGKL
                     KLFKWVIK"
     gene            24392..>24687
                     /locus_tag="PLO70_07785"
     CDS             24392..>24687
                     /locus_tag="PLO70_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74402.1"
                     /translation="MKKLKKFAIVFFLFIAAFAVYVEIANRNSKNMTYRQKILKAVYP
                     VLMWASGFIGKSTKKLSNDTVRPPVPFYSLKGESGNGDTIDFNSFQGKKVLLVNT"
BASE COUNT         7450 a         4721 c         5383 g         7133 t
ORIGIN      
        1 gtcaagaagt ttgtagctat gatagctact aaaataaact ttttcataat tgttggtttt
       61 gattataaaa ataaataacg acttgatatt gatatagggt gttttatttt ttataggggg
      121 tgattaaacg gtgggtgcca ataatgcaca acgtatgtat tatcgctatt atatgaatct
      181 ataatttttt aaaaaattaa caatcaatgc aaaatattat aaatacgact tgcgacaata
      241 tccaattgta aacgtttaaa aacaattgta cacggacatc ttcatcctgc tcaatcataa
      301 agcctccccg ttggttcgta cttgcttcgt gtcttagccg aggtataggc gtacttaatg
      361 caattccatt ttgggaagtt acacggatta gttttggtaa acaataccac tttcaggtaa
      421 ttgttgctgt aagatcagcg gatattttgc agcaatcttt ctttcacccc tccaaaccat
      481 tctgttttta aaatgctcag gatgatactg ttccttcttc ctccactggc agtgggcata
      541 tggttgcgca ggataccttc ttccacacag ccaatagcct tcatggcagc aatgctcccg
      601 gcattattgg catcggcacg aaactctaaa cgttccattc cccattcttc aaacacataa
      661 gtgagtaata ataatttgca atgcctgttc aacccggtcc gctgaaattc ttttccatac
      721 caggtgtagc ccagttgtgt ggttagccag gattgctgaa tatcataaaa tcttgtactg
      781 cctgcatagc ggttggcttt tttatcaaac acaataaaag catattcttt tttatcctcc
      841 cggttcttaa tggccatggt aatatagttg cggagattgt cttcacccgc cgccgttacc
      901 aaaccatatt tccatatctc gggttcgttt aaagagaagg gcaaaagctg ttgaaagtca
      961 ctttcctgta tgggtcgcaa taacactctt tcgttttcaa gaacaggttg ttgctggaaa
     1021 atatctgttg gcattgcagc ggctttgttt tttaatgatt gttttttttg gaccgattaa
     1081 ccaggatata aatgaccagc cctgccatta ttgctatacc aacatcccgc agttccctta
     1141 gcctggaact gctgagcagc catagcgtaa ttaagatccc ggcaacggca attatgttgc
     1201 catgctttaa tttataaaaa ttagcctggt ccggattttt tttgcgtagc aggataagtg
     1261 tggtgcatac aataccatag atcattactc ttgtaatggc actgagtgag gcggctgata
     1321 aaaaagagta attgagcgaa acaataatgg tgatgctcat aaacaacaac agcgaccagg
     1381 tgggggtttt atatttcgga tggatgaagg aaaacaaccc gggaaactgt ttttcttcgc
     1441 tgaatgcata gggcaatcgt gagcctacca gcataatggc atttagcgtg ccggctactg
     1501 aaaagatggc gccgatggca atgatgctgg cgccgctttt acccataaac aaaccggctg
     1561 cttctgctaa gggcttgtct gatgatgcca gtgccggtaa tgtaccgatg ctcaccacct
     1621 gtatcagtat ataaatagtg gtaatgaata ctgcggcgag cagtaatgca aagggcagat
     1681 tcttctccgg gttctttact tcgccgctgt ttaccagtac cgattcaaaa ccgccgaagg
     1741 caaacaccag caataataca gtagaagaaa aggaggaaaa atcagggata tgctttattt
     1801 catactgtgc aggttcaata aaaaaagaac caacaacaat aaaaagcaac aggggcaaca
     1861 atttgctgat ggttaaaaaa ttattgatcc gggtcgtgtt tcttattccg atgtgattga
     1921 tatacgccaa gagcagcgta aataaaacga ttacaattat ccttgcttcc ggcaatgtga
     1981 accaggcagc aaaaacagaa agataggaaa ccagtaagtt gatcaaagcg gcgtaagtaa
     2041 tgaatcttgt taccagcaat aaccagccgg tcagaaaagc cggtaatttt ccgaaggagg
     2101 aaagggcata gagataaggt ccgccggttt tactgaagcg gctgcttact tccgcaaaac
     2161 aaagtatgat gacaaaaact gcaacggcac aaaccaaaaa tgcagcaata ctgtacacac
     2221 cggtttcttt aaaaactttt gccggcaatc cgaaaatacc agaacctatc accccattga
     2281 tgatcaatag taccatcgcc catttgctga tactccggtt aagcatgttg aaaagatagg
     2341 gaaataatca gtgctggtag gtagcaggga aatgatcagg agccggttct ttttatatcc
     2401 gcccccaatt ttctcaaccg tccgtcaata tcctggtagc ccctgtctat ctgttcaata
     2461 ttctggatca cactttttcc ttccgcactt aatgcagcaa ttaataaagc cacaccggca
     2521 cggatatccg gagaactcat ggtgatgccc cgcaacattt gttctctttc caaccctatc
     2581 actacggcac gatgcggatc gcacaacact attcttgctc ccatatcaat cagcttgtct
     2641 acaaagaata aacgactctc aaacattttc tggtgaatga gtacagatcc tttggcttgt
     2701 atcgctgtta ccagcacaat acttaacagg tcgggagtaa aacccggcca gggatgatcg
     2761 tagatggtca tcacggatcc gtctaaaaaa gtttgtatct cgtaactctc ctgcgagggg
     2821 atatgaatat catcaccatt gaattccagt tgtatcccca gctgttgaaa cttttcagga
     2881 atgataccca aatgatctat acctgcacct ttgatggtga tatcgctttg tgtcatagcg
     2941 gccagcccga tgaaagaacc tacttcaatc atatcgggca gcatactatg ttctgtcccg
     3001 ccgaggtagc tgactccttc aatggtcagt ttattgctgc caataccact gatctttgca
     3061 cccatccggt tcagcatctt gcatagttgc tggatataag gctcgcaggc tgcattgtaa
     3121 atagtagtgg taccattggc catagaagcg gccatgataa tattggcggt gcctgttacg
     3181 gatggttcat ccagcaagag gtatgcaccc tgtaactgcg gcgcttccag gtgaaagaat
     3241 ccatcctctt catgataaat aaaagaggca cccagttttt caaaaccaac gatatgtgta
     3301 tctaatcgcc gccttccgat tttatcacca ccgggtttgg gtataaatgc ttttctgaat
     3361 cttgccagca tgggtccggc cagcatgacc gaaccacgca gcttgccact tttcttttta
     3421 aaggcatcgc tgtggaggta atcaatgttc acatcattgg cccggaaggt acaggtgtca
     3481 cggctggttc tttcaatgtt cacattcata tcaccgagta actctatcag caggttcaca
     3541 tcaatgatgt cagggatatt agtgattgtg atcttttccg gcgttaacaa taccgcactg
     3601 ataatttgca gggcttcgtt cttagcaccc tgtggtatga tctctccctg taatttttta
     3661 ttgcctctta cttcaaagga atgcatatgg gttaattgat ttgcgtattg gtagatccgg
     3721 ttcaggaatt cattgtgttt gcaaagtaaa tacaaactgt agtgggggca ataaaaaaag
     3781 gttgatcaga aagcctggtc aaccttttaa ctatttaatc tcccggtaac tatcgggata
     3841 gcttaatacc gtttcttttt aaaaccacca ccgccgccgc cgcggttatc acggccatca
     3901 tttctgcgct ggccgccacc accgccacca cgctgctgga attttccacc cggtcttttg
     3961 ccatagccgc cgcggttatc ccttccttcc tgctgcggac ggaatgcttt tacaaatgga
     4021 gtattggtaa aagttaactg gccattggta atggaagaca gttcactttg tatggcatca
     4081 tcatgcacaa tttctttatg ccagttatta tacgccagct tcatgtaata cgctacggca
     4141 ttggcaaagc ctaatttctt ttcaggattt tcttcctgca gtgctttatc aatgatcact
     4201 tcaaggttct tacccaggtg tgaaaattta ggatgacgtt tgggataagg taatgggtca
     4261 ggcttcgcct ttaatgtttc ccgggtggga atagggtagg gagattttac atccatttta
     4321 aaatcagaga tcaggaataa gtgatcccat aatttatgac ggaaatcttc tacatttttc
     4381 aggtgcgggt ttaaaaatcc catcaattca atgactgcct gggcatttcg ctggcggcgt
     4441 tcgggatcct caatcgtcat tgtatgttct accattttct gcacatgacg gccatattcc
     4501 ctcatcgtca ggtaatttct cgtggtattg tactccatgc tatctatatc cattgcaaca
     4561 aatgtagaga aagaagtttg aatgaagaag tccgggaagg gcaaaaagaa aggggccccg
     4621 aacctgggcc ccttttacaa gccaccatcc tcttccacct aagaggtcat ttaatgaaaa
     4681 gttgattgac actggatagt tggctcgtta cgtctgtaag cctcaaccag taaactccgc
     4741 cttgtaacgt ggcaacctgg tgataagaag tacctgaaac cactatctct ttctgcatca
     4801 ccttttgtcc ctgcgaattg taaatctcca gcatcatttt tccgccctca ttattatcaa
     4861 atttgatacc aacaattcca tttgacggat tgggagttac aataaatttg ggcagggatg
     4921 aatttttaag agacacctgt ttaacatcgc tgaaccgggt atagccatta gcatatacct
     4981 gttttacacg gaaaaagaac tctccgcttt cgttttgcct tgttgtataa ataaaatcat
     5041 atatatcatt actggaagca tttttggcaa ccgttcctac ggtgctgaac tgccttccgt
     5101 ccctgctcat ttcggcttca taatgataag aggcagaagg atcattaaac ccggaccaat
     5161 tcagctttac actgttatcg gcaattttat taattgcgaa attgcgtaaa tgcaggggaa
     5221 ggacgtagcc cgggcaggta cagtattcaa aacgaaaacg gacaatagca gaagtggcca
     5281 ctgtactgtt atagttaccg ccggtacagg ttacattggt aaaagcagta atattataat
     5341 tataagttat actgtcatga ccatagaact ggaacagtga gtccaggtta gtaatggttt
     5401 ggcagacttg tacggcgttc aacaatgtat cttttgagat ggatataaaa tccggaccag
     5461 aaccaagtgt accattagta ggaccaagtc catacggacc atagtgatga ttgatactat
     5521 tatttaaagg agatgataat cccgggcctg atatctggtc tgtcctgatg taatagatat
     5581 cagctgtttg tggtgaagct gaattatttt ccacactaac ggagtcaacc accccggtaa
     5641 tagaaacgca aatacgcaga caggtcacca tacccgccat aggatctgcc tgtggcactt
     5701 tgacctgcag ggtattgata ccggggggag tatttattgt tgtgtcatac aatgtagttc
     5761 cggaagggaa accgtaagga cattgggcat tcgctgtgtt aaaacctggt aaattcatca
     5821 ggataaaggt tccaacagca ataaagaatt ttgagtaaga gtttttcata ggtcggtgtt
     5881 ttggtttcca ctaattagaa agtactgtaa aagtagagca gttcataatg gagaacaatt
     5941 gtagtaatgc cctattttaa ttccggtatt tcatgaatat ccacagaaaa tactcctgta
     6001 aaaatctaca tacggggcgg gagaaacgga aagaaaaata acagaacagg tttcgctttt
     6061 tacgacacga aaatgatttt tttgcagcag tgctggtgtt tatttacgct caagtcagac
     6121 gggcagcaac tacctttgtt gttactgatg cggatcgctg taaatacaag gttcttattg
     6181 aaagattacc tggaagggta tggtaatttt ctgtatgaaa ctttcaggcg gatcacggag
     6241 aatcacccgg agcatgaatt cattttcatt tttgacagac cttttgatat tcgttttgtt
     6301 tttaaagcca atgtaacacc ggtggtggca ggacctgcgg caagacatcc gttactctgg
     6361 aaatactggt atgacataaa aataccggcg ctgttaaaaa agtataaagc cgatattttt
     6421 gtttcctgtg atggtttttg ttcactcaat acgaaggtgc cccaatgcct ggtagtacat
     6481 gacctggctt ttattcattt tccgtcgggt attaaaaagt cacatttatt attttataaa
     6541 cgatttactc cgaagttttt ggctacagca atagctattg caacggtatc acaattctca
     6601 aagaatgata tcgtaaggca gtataacaca gctgccggta aaattgatgt ggtgtataat
     6661 ggagtaaaag aaatattccg tccgcttagc tatgaaaaca aagaggcagt gaagaaaaaa
     6721 tatacggagg gcaaagaata ttttgtgtat gcaggggcca tacatcctag aaaaaattta
     6781 atgaacctgc tgaaagcatt ttctgttttt aaaaaacgtc agcaaacagg aatgaagctg
     6841 gtactcaccg gcaggctggc ttggaaatac gagtctttta cagcaagcct gaaaacgtac
     6901 aagtacagga atgatgtggt gttaaccggt tatgtacccg aagaagaatt ggtcagtatt
     6961 atcggtgccg catacggaat ggtgtaccct tccttatttg aaggatttgg ggtgccggtg
     7021 ctggaagcta tgaaatgtga agtgcctgtc attacttcct ccggttctgc catgcaggag
     7081 atagcaaaag aggctgcttt gtatgcagac ccgggcaatc atactgatat tgctgacaaa
     7141 atgatgctgt tgtacaaaga tgaaaatctg cgaaaagaaa tgattcaaaa aggtaaacag
     7201 gtgataaaag aatacaattg ggataacaca gcagcacttc tctggcagtg tatccttaaa
     7261 gcacatcaat gaatttggtg ctgcccttct tgccgtgtgc ttatctttgc atcctcttaa
     7321 tcataattat gaataaatcg acaattacta ttgatgtgca tacggatgag aaccggattc
     7381 cagcttccat tgcgtggagt gcaactgata ccagtatcga aaatgcgcag aaggctaagg
     7441 ccatgatgat atcattttgg gatggtgcag aaaaagcggc tttacgcatc gacctgtgga
     7501 caaaagatat gatggtagat gaaatggcag atttctttta ccagacaatg atgaccatgg
     7561 cggatacata tggccgtgcc actaaatatg ctgaccaggt ggaagaaatg aagacatttg
     7621 caaagggctt ttatgaaaag ttcagggaaa aacaattgaa ggagaataaa gcctgatttt
     7681 aagaacagaa aagcaggttt acggaaagta actctttgct ggttcagtta agcaaattaa
     7741 tagtacttaa taaattttta tatgaacctg gaacaaaaaa taatggctga cttaaaagca
     7801 gctatgctgg ctaaagatga agcagcatta agaagcctgc gtgctataaa agcggcaatt
     7861 ctgctcgcca aaacagcgga aggagccggt ggtgagctaa aagaagaaga ggagactaag
     7921 ttgttgcaga aaatggtgaa acagcgtaaa gactcgctgg aaatatttca gcaacagaac
     7981 cggcctgagc ttgcccggaa agaagaagaa gaaatcgctg tgattgaaaa atttttgccg
     8041 aaacaacttt cagctgaaga gattaaagca gcgttgacaa aaattattgc tgaaaccggt
     8101 gcttcttccc cggcagatat gggtaaagta atgggagcgg ctaccaaaca actggctggc
     8161 aaggccgatg gaaaaacaat ttctgcattg gtaaaagaat tactggcgca ataactggta
     8221 ttttaataac ttattggcaa acaactgcac taattgttac ctggactacg ttaaactcct
     8281 gaatgattat tgatctcatc ttaggcgtta ttatcttaat tgccattttt aaaggttatc
     8341 agcgtggcct tatcatagga ttattctccc tggtgtccgt tattattggt ctggctgcag
     8401 ccatgaaact ttctaccgta gtggcaggat atatcggaga ggctgtgaac gtttctgatg
     8461 agtggttacc tgtaatttca tttgctatag ttttcattgt tgttgttctg ctgatccgtt
     8521 tgggtgcaaa tgccatcgaa agaacggtgg aggtagtaat gcttggttgg ttaaacaaac
     8581 ttggcggcat tatattttac gttgctattt atactgttgt ttttagtgtt ttactttttt
     8641 atgctgagca ggtaaacctg gtcaggtccg aaacaatcga aaaatctata tcctattcat
     8701 ttgtccagcc ctggggtccc agggttatca atagcgttgg ttccattgtt cctttattca
     8761 gggatatgtt cgccgaattg gagctttttt ttgaaaatgt atcaaaagag atcccggttc
     8821 gatgatatct tagcacaact tctttcaccc cgcacatata tggcttttgt ataattatta
     8881 caggtgctgt gacaggggta ataaaaacat ctcatcaaaa aaaggctgtt gcccctatat
     8941 ttgcgataat cagctctctt ttgtgccata aacagctgat tttttgcgct aattgattat
     9001 tttagcaatc agagtttaag cttagattaa ctgcccatgg attacgaaat caaacaacag
     9061 gataaataca aatttgtaga agaaggtagt ggagaaccat tggtgctttt gcatggcttg
     9121 ttcggagcat tgagcaattt tcagagtctg atagaatact tccgtcatca ttacaaagtg
     9181 gtggtaccta tgttaccact gctcgaaatg gatatactgc atacttcggt gggcggattg
     9241 gccaaatttg taaacaaatt tctggatgcc aggaaattag atgcggtaaa cctgttgggt
     9301 aattctttag gtggccatgt ggcacttgtg catacactaa aacatcctga aagaataaaa
     9361 tcactaattc ttaccggcag ttccggttta tttgaaaatg gtatggggga tagttacccg
     9421 aagcggggcg attacgaata cattaagaaa aaaacggaac tgacttttta cgatcccaat
     9481 acagcgacca aagacctggt ggatgaagtc tatagtattg tgaataacag attaaaggct
     9541 atcaagataa tagcattggc aaaaagtgct attcgtaaca atcttggcga agaactgaat
     9601 caaataaagc aacccacctt attggtgtgg gggaataacg atacaattac cccacccttt
     9661 gtaggaagag aatttaataa gctgattccg aacagtgagt tgcatttcgt agataaatgc
     9721 gggcatgcgc caatgatgga agtgccggaa gagttcaatg ctatcctgca caaatttttg
     9781 aaaaaactca atgagcctgc aacagttgcc tgatcgttgt tgtcttgctt tcactacgaa
     9841 acaaaaagtt taatagtgaa gaaataataa agccgctatc catgttaacc agagaacttg
     9901 aatctcagac actcccttac ctgcacctta ctgacaaggt gtaccaggca ttgcaactca
     9961 tgaatgataa ccatgttacg cacctgccaa ttgtagaagc tgataaatat ataggtctca
    10021 tcagcgagga agatctgttg caggcagaaa atgacaatgc tgaactccag acactgcaac
    10081 agtcatttgc caacccatcc gttaaagggg acgaacattt ccttaaagct gttcaggtag
    10141 ccgctgagaa tggtcttagt gtagtaccgg tgctcggaga cgataatgaa ctgatcggta
    10201 cagttgtgta taatgacctg ttgaaacatg cctccgagtt tatgagtctg aatgaacccg
    10261 gtggcctgat tgtgctggaa atggaaagta accagtattc ctttaatgaa atcagtaaac
    10321 tggtggaaac caataatgcc cagattaccc agttgaatac tactaatgat gctgaaacag
    10381 ggttgatgca ggttaccctc cgtatcaata aacccgaggt ttccgatatt gtagctacat
    10441 ttcagcggta tgaatacaat gtaaaatact ttttcgggga agaactgtat actaacgaat
    10501 tacggagcaa ctacgataac ctgatgaatt atctgaagat ttaatcactt ttacccgtat
    10561 tttacatagt taaaatataa atccggttaa tttagaattt tacggatctg taaatgatgc
    10621 gggagaaaaa acatagtatt tggctggtag ctgctttaat tagttgctgt tccataacag
    10681 cgcagcagcc cgatatgcca ccacccgttg ctgattcctc tgttgctgct gctgccaggt
    10741 ttattgttgg cgaaattttt atcaatggga ataggaaaac aaaggccagt attattctcc
    10801 gggaaatacc ttttcatccg ggcgaagaat acccgttgca ggacctcgtt aaaaaatttg
    10861 aagatgccag gcgtcagtta atgaatacga ccctttttca cacagtagtg gtagctgcca
    10921 agagctttga agggaataaa attgatattg tagtagatgt aaaagaaaga tggtatctct
    10981 ttccggtgcc ttacctgaaa ccggttgaca gaaatttaaa tcaatggata gtagaacaaa
    11041 aagcaagcct gagcagggtt aattatggag caaaggtttt gtacaataat gctaccggga
    11101 gaaatgataa attcagacta tggcttattg ccggctacac caaacaggtt tccatcagtt
    11161 acgacaggct ttatattgat aaggctatga aatggggaat gaaatttggt ctttctgccg
    11221 ggaagaatcg tgaattgaac tacaatacta ttaatgataa gcaggtcttt ttaaaggata
    11281 atgatcgcta catcagaaat tttgtaaata catatgctga acttacttac cgtagagcga
    11341 taaaaacccg tcatagtttc ggaattgcct attcttccga acaggttcag gacaccatag
    11401 taacacttaa tcccacatat tttaaatcgg gccgaaaccg gattaaattt ccggaattgt
    11461 attattccat ggcctattac gatgttgatt acattcctta tccaacacag ggctatgccg
    11521 ccaatgtgtc aatcagcaaa agagggttta ataataccat aaatgtctgg cagcttcatg
    11581 taaaagggtt tggcagctgg catttatcgc cgaaaacatt tgtaaacctt tatgcgtatg
    11641 gaggtataaa acttcccttt aagcaacctt attttaatca acggtttttg ggctatggcg
    11701 atgtgtttat gcaggggtat gaatactatg tagttgacgg ggtggccggg ggttttctaa
    11761 aagccacgtt agcccgggaa atgctgaact tcaatatcag gatacctccc cgaaaaggaa
    11821 aagaagcgga gcgaatccct gttcggattt ttggtaaaat atatggtaat agcggttatg
    11881 tacataaccc ccagccgggt gaaaataatc ttagtaacag aatgttgaac gctgccggca
    11941 ttggtatcga tattctaacg ctttatgatg ttacattccg gtttgagtac tcattcaacc
    12001 agttaggtca aaacggttta tatttacacc gaaagacgat tttctaaaaa tgaaggcagc
    12061 tatttacagc agggtttttg aagaagtgca taaagaagag gttcagcttt tttttgatga
    12121 actggcaagt cagaaaattg agccggtcat ctttctaaat ttctttgatc agataaaaaa
    12181 tgagatcaac ctccctgccg atacaaaaat attttctctc tccgaagatt taacggatga
    12241 aattgaattc atcatcagcc tcggtggtga tggtactttg ctggataccg taacccttgt
    12301 cagggataaa aagatatcca ttatgggtat caattttggc cgcttggggt ttctggcaag
    12361 tattggcagg gatgaagtta aaactgcagt aaaagccata gcccgccgaa cctatgtgga
    12421 agatgcaaga acactaattc atgtggatgc agatatgcct ctctttggca atgtggctta
    12481 tgcattaaat gagttttcta tccacaaacg ggatgtggcc tctatgatta aaatacatac
    12541 gtatgtgaac ggggagtttt taaatacata ttgggctgac gggttgatcg tggcaacccc
    12601 taccggctct actggttatt ccctgagttg taatggtccg gttgtttttc ctgattccgg
    12661 gagttttgtg atcactcctg tggccccaca taatcttaat gttcgcccca ttgtagtgcc
    12721 ggatactaat attatatcct ttgaggttga aagccgttcg gatcaaatta tttgtgctct
    12781 tgattcacgc agggaaatcg tccataaaaa tgttaccctg gctgttagaa aagagagctt
    12841 tgacattaac ctggtccggt taagtgagaa taattttttg caaaccctac gcaacaaatt
    12901 aacctggggt ctcgacaaac ggaattaaaa cacacaccaa ataataagaa aatagctact
    12961 tttagcgttc tatttaatcc tgaccatatt tttagttatg aggaagaaat ttttaggcat
    13021 agttgttttc ttttgcgttg ctgtttttgc tggccgtcag cttcatgcac agaactccgt
    13081 agttcaggaa ggagagttcg gatttggtgt cggagccggg cattatttcg gtgatctgaa
    13141 tactaaagca agattgaaca gggccaaatt tgccgctaca gcgttttttc gtaaaaattt
    13201 tggaaactat attgccgcaa gagttggagt ttcctttgcc caacttggct attcagatat
    13261 atacaattcc tataataagc aaatgtttag ccgaaacctg agtttcaaca gcaaggtgtg
    13321 ggaattgact ttacaggggg attttaattt ctttcgcttt atgccggggg agccagaata
    13381 tagttttaca ccctacatta catttggagc aggtattttc agttacgatc cctatgcata
    13441 tctccggggt gagaaagttt ttttacggcc attaggaacc gagggacagg gaagcagcct
    13501 gtaccctgac cgtaagcaat atagttccat ggccatctgt atacctttcg gtggcggaat
    13561 taaatattca ttgaacgaaa gaatcaatat tgcttttgaa gtattgcatc ggtttactaa
    13621 taccgattac ctggatgatg taagcaagac ttatgttgat cctgccgctt ttccactcaa
    13681 cccggatggc agccagtcca atgcacagct gcttagcgac agatcatatg aagtaggccc
    13741 tccgatcggt attccaaaca ggcaacgagg taacagtaaa caaaaagatc agtttgttac
    13801 ggcaatgttt catgttacgt tcaatcttca atcgtataag tgcccaacag ctaactaaaa
    13861 tctcacataa ggcctctttg agcagtagta tcattattta tcattggttt atactattat
    13921 gatgaagtaa tagtctgcca gtattatatt ttattattta ataattacta ctatgcgcct
    13981 caccacaatc attctcagtt caattgtaat tgctgttttc agtctcacaa cagcttttaa
    14041 gactacggac acaggttcct ggacattttt aggagataaa aatgtaggct ttggagttga
    14101 ccatgatgtt attcattttg gcaactggaa agacgatgtt cgccaggtta aactgaaaat
    14161 tacagatggt cctttaaaaa tgtacaggat gaatatccat tttgataatg ggtcagtaca
    14221 ggaagtgact ttaaggaacc gctttgccca gggaagtgaa agccgtgtta tagaccttga
    14281 tggaggcctc cgtcatttga ataagattga gttttggtat gaaaccaaag gatttctaag
    14341 aggaaaatca agggtagcag tatggggaag gaactaaatc tggtggtaga aaaaataagc
    14401 tccaggcaga atgaaatgcc cgtataatct catgtttggc gattttttat tgttttaatt
    14461 ataccataat cccgaaaatt ggcgatacct ttgtaccccc tgtttaaagg gggtttttta
    14521 tgtccgattt gctggaaatg attgatagag accgtctccc caggcacatt gccatcatta
    14581 tggatggaaa tggcagatgg gctaaagaga agggggaaga ccggcttttt ggtcattttc
    14641 atggcgtgga aagtgttagg aatattgttg aaggatgtgc agaattgggg gttgaatacc
    14701 ttacactgta tgcattttct accgaaaatt gggacaggcc ggaatatgaa gtagtgggtt
    14761 tgatggagtt attggttagt actatccgga aagaggtaga gagcctgaat aaaaacaata
    14821 ttcgcctgca tgtgattggc gatatgagta tgttgccgga atatgcaaaa aaagaactgg
    14881 atgaggccct tgaaataaca aaggacaata aagggctgaa ccttataatg gcattgagtt
    14941 atagcggcag gtgggagttg ttgaatgctg taaaaaatat tgcttacgag gtaaagcagg
    15001 gacggctgaa tgtggaggag atcaaccagg atacgctgca gcattttctt tgtaccagtg
    15061 gctttcctga tccggaactg atgataagga ccagtggcga gtaccggatc agcaactttc
    15121 tgctatatca actggcttat gctgaattgt attttaccaa tgttcgctgg cctgatttca
    15181 gaaaggataa tttatatgaa gctatactgg actatcaaaa cagggaaaga aggtttggaa
    15241 aaaccagtga gcaactgaca gatgctaatg aaagttcaaa attgtgaaac cgggaacggc
    15301 tcgttctaat tcagggtttc tttaacaaag actttcgata ttaaccttta atttgccgcc
    15361 ggatcaaaat ggcggtattt tttataaacc gcggaaaata aactaattaa gtcccgccaa
    15421 ggtgggaaag gacagaccag aacaataaaa catgcaacga tttttacaaa ggcttggtgg
    15481 tactttaatg actgcagtaa tgtttgtatt tacggtttct gcgcaggaag tacccgatac
    15541 tacaaagcca acctcaatag atccaaagct gatagaatgg caaaatgccc gcattgtaaa
    15601 agagtatact attgctgaag tgaatattac aggtatccgt tacctggata cttcaatagt
    15661 gtattcaatt gccaatcttc aacccgggga taagtttgta cacccgggag ctgatgtatt
    15721 tggaaaagcc attagtaatc tctggagaca aaaacttttt tccaatgttc agatttatgt
    15781 aaccaggatt gacggtgata aagtatggct tgaaattaat ttacaggaac gaccacgact
    15841 tggcagctat aagtttgtcg gcattaaaaa atcagaggaa gaagatatcc tggggaaaat
    15901 aacactgacc aagcaaacga tcataacaga aaatacccgc cgggaattaa ctgaaaaaat
    15961 aacgggtcat ttttcggaaa aaggctatcg taatgtaaaa gttaagatag aggaatctcc
    16021 ggatcctgct tttgttaatt caaatgtttt aaccataatt gtcgacaaag gtggaaaggt
    16081 tcgtatcaat gaggttggtt tttacggtaa cgagaacatt gaagacctac ggctgaagaa
    16141 acaattgaag ggaaccaagg aaatgagtaa gctgaccttt tacccggata aagctaccag
    16201 cccttatgga gagaacaagc cattttcctt taatcaatat atgcgggatt ggggatttct
    16261 ttcactcagc aaaactaaaa tgttgcttga tccctatttt cgtttcaagt tgttcagcgg
    16321 ggcgaagttt gatgcaaaga aatacgaaga agacaaggag aagattcttg attattataa
    16381 ctcgatgggt tatcgtgatg cacagattgt cgcagatacg caatatacaa ctgcaaatgg
    16441 caacctgaat gttgatctga aagtggatga aggcagaaga tattattttg gcaacatcaa
    16501 ctggaagggt aatgcgaaat attctgattc tgtactgaat gttatcctcg gaatcaataa
    16561 gggagatatc tacaatgttg caatcttaaa taaaagactg ggtaaagaag cttcgcagga
    16621 cggaggtgac attagcgggt tgtatatgga tgaaggatat cttttcttcc gggctgagcc
    16681 gatagaaact gcggtctata atgatacaat tgactatgag ataagaatta tggaaggccc
    16741 gcaggcaagg ataaaaaatg tgaccattgc tggtaacgaa aaaacaaaag attatgtgat
    16801 ccgccgggag ctgaggacca tacccggtga gctatttagc cgctctgatc tcatccggtc
    16861 tcagcgggag ctgggtaacc tgcagtattt taaccaggag actattaatc caggtgttgt
    16921 cccaaatgca gaagacggaa cagttgatat taactggaag ctggaggaaa aatcgtctga
    16981 tcagttggag ctatcggcag gttggggtgg cggaatcggt cttaccggaa ccctgggggt
    17041 aacctttaat aatttttcaa tcagaaatat ctggaaaaaa tctgcctggg atcctttacc
    17101 aaccggtgat ggacagaagc tgagtttgag aatgcaatcc aatggccgtg cctaccgttc
    17161 ctatagtgct tcttttactg aaccctggct gggtggtaaa aagaggaact cattgaccat
    17221 tggttttaat aacagtaagt attcgaatgc atttgatcct tttacggggc aaatagacag
    17281 ggcaaggtcc gattccaatt tcttgaaaac aacaggagtt tcagtgtctt tgggtaagca
    17341 gttaaaatgg cctgatgatt atttcagcct tgtttataca ttcaatgtca cgcaatataa
    17401 acttcgcaat tatcctattt tcgaccagaa cttcacgaat ggaacatcaa ataacgtaag
    17461 tttcaaaata ggattacagc gttcttctgt cttcaatcct atttttccga ccagtggttc
    17521 aaacttcctg gccagtgttc aattcactcc gccatattct ttattcaata agaatattgc
    17581 caaatctgac aacaagtata aaaatcccga ataccataaa tggcgtttca atgcagaatg
    17641 gtatgtgccc attggtaagc ctttgggtgc cgaaaagaac aggcagtttg tgttgaaaat
    17701 ggcagccaag tatggcttta tgggccggta taataaagac cttgattatt ctcctttcga
    17761 aaggttccag gtgggtgatg caggtcttac caataatttc ggactactcg ggtatgatat
    17821 catcgcacac aggggttatc ctgtttacca gtcatcagat ccgacagtaa atcctgatca
    17881 gcaaagtgcc agccagttct ttacaatatt caataaatac cagttagaaa tgcgtttccc
    17941 gctggtaacg aatccaagca gcaccattta tgcacttact ttctttgaag ctgcaaacgg
    18001 ttggtataat tataaggatt ataatccctt ccgtcttcgt agaagtgttg gagtaggtat
    18061 gcgtttcttc ctgccgatgt ttggtttact agggtttgat tacggggttg gtttggatcg
    18121 tattcaaccc gggcagagtg ggttgaaagg ggcgtcccgc tttaccttta tgctgggctt
    18181 tgaaccggat taatcgttaa cacataaata gaaaagtatg atatgagcca tgattttttg
    18241 aggtgatttt tataaactgc cggtaaaaat ttttatggcg aataacatgt gaccttttta
    18301 aaacaataaa aaaattaaca catgaaaaag atttttctcc ttgcctgtat gcttgggttt
    18361 ggcattgttg gtttttctca gaaatatgcc attattgata cccgctacat tttggacaaa
    18421 atgccagact ataaagaggc gcaaaaacaa ctggatgaaa ttgctgcagg ctggcaaaaa
    18481 gaaattgatg ctaaacaggt ggaactggac aaaatgtata aagattacga gggagagcag
    18541 gtaatgctga cagaagaact ccgtaaaaaa agagaagatc agctttttaa caaggaaaaa
    18601 gacctgcggg atcagcagcg caaacgcttt ggttttgagg gagatctgtt caaaaaaaga
    18661 caggaattga taaaacctat ccaggataaa gtgtacaatg cagtacagaa aatggcggta
    18721 ggcaggggct atgattttgt gctcgataaa agtgagggaa ttaccattat atttgccgac
    18781 ccaaaactgg acaagagtga agatgtgctg aaagatttgg gagttcgtta aatgttaaaa
    18841 aatgtgaagg cgcaactaac tgccttttat tagaatcata taaagcaata acaaaacaaa
    18901 ccagatgaaa aaaattcttt cagtaattgc aatagtgggt ttgctgtctt tttcagttac
    18961 tactaatgcg cagacaaaaa tcggttacat aagtgttgac aatgtcattg gacttatgcc
    19021 agaagcaact aagatagatt ctttacttga acgttaccag gctgatacat taaatcccga
    19081 gtatgcttca ttggtacagc gttaccagtt tgacgatagc ctttaccggg actcagtaaa
    19141 aacgccaaag gctgttcgtg atgagatagc taaaaagtta ccaagttaca tttaccagat
    19201 tcaaaactgg caacaaatcg taaaccaggc gctggaagct aagcaaaatg agttattatc
    19261 tccactatac cgtaaggcat atgatgctat caaactggta gcaaaggaaa agggctacac
    19321 tcatgtaatg aataaagaag cgttactggt tgctccggat ggtgatgata tgctgaaagc
    19381 tgtggcagca aagctaaaga taacgatacc cactaatcca cccccggcag ggaaataacc
    19441 ggatttttca aaatcatata ttaaccccga ctttctggtc ggggttattt tttaccatcc
    19501 cctgcctact tttgctgtat gcaacaacct ggtcctatcg gtatttttga ttctggctat
    19561 ggcggactga ccgttttaaa agaaatagtc aataagcttc ctcaatatga ttacctgtac
    19621 ctgggcgata gtgcgagggc accctatggg aaccgttctt ttgacacggt gtatcattac
    19681 actctacaat gtgtgaactg gttttttgaa caaggttgtt cattagtaat tcttgcctgc
    19741 aacactgcat ctgcaaaagc gttgagaacg attcagcaaa acgacctgcc gcgaatagca
    19801 cctggtaaaa gagtattggg ggtaatcagg ccaaccgcag aaatcattgg taatttttct
    19861 gaaacagaaa gtgtaggagt tctggcaact actggaacag taatgtcaga atcataccca
    19921 atggaaatcg ctaagttctt tccggagata aaagtatacc aggaggcctg ccctatgtgg
    19981 gtgcccctgg tagagaataa tgaatacgaa agtgatggtg ctgattactt tattaaaaag
    20041 aacctccgta ctatttttga aaaaggagag gatatagatg ttatactgct tgcctgcacc
    20101 cattatccac tgttgaaagc taaaattgag gaatacctgc cggttggtgt aaaactgctt
    20161 tcacaagggg aaattgttgc agccagcctg gcagaatatt tacatcggca cccggagata
    20221 gagagcaggt gccgtaaaaa tgggcaaaga gaattctata cgactgattc cactgccgat
    20281 tttgacaatc atgccagtac attttttggt gaaaaattac aatccaggca tgtagacctt
    20341 gatgctgcaa taagttgatt ttcaatagcc tgaaagagaa attgaatacc caaatatagt
    20401 tgtgtttatt gctgcagcct ttgttttatc tgtatcctgc agcagtcttt caaatttaat
    20461 ctattcagtc gttttacttc tggctttcat cccttacctt tgccgtcctt tcacaaaaca
    20521 gcgggtgtgg tgatctgctg tgaatatgaa acctgggccg ttctcccggt ccgtatttca
    20581 aaaagtgaaa aaagagaaat aattatgaaa cgtacattcc aacctcatcg caagcgtcgt
    20641 aaaactgttc acggttttcg taaacgtatg gaaactgcta atggccgcaa ggtattagcg
    20701 agccgccgtg caaaaggccg taaaaagctg actgtttctg atgaaagaaa attaaagtaa
    20761 aaagtaccgc cgttatttca acggaggttt caataatttt atagctctgg tttttccgga
    20821 gcttttttat ttgtggtaaa acaattcaca cttggtaagc aggagcggct gaaaagccgg
    20881 aaagctattg agcaattgtt caaggacggg caacgtttta cgatcactcc ttacagggta
    20941 ttttatagga aagcaggtaa acccggatta cagtttggcg caggggtcag ttccaaaaat
    21001 tttaaaaaag cagtcgacag gaacagggta aaacgattaa tgagagaagc gtatcgcctt
    21061 caaaagaata atttgcaaaa gcacttgaag gatagcaata ccggactgaa tcttttcttt
    21121 atatacacag ggaaagaatt gccttcttat gatattatat atgagaaaat ggcacttgcg
    21181 ttacaaaaat taaccgattc gattgagcag taaaggacct ataaataaat tcttcaaagg
    21241 actggggcaa ctcttaagcc tccctttcat agcactgata aagatttatc aatgggtgat
    21301 ttcgccatac ctgggcccta aatgccggtt tacccccacc tgctctcatt atgcagcaga
    21361 ggctttacaa aaacatggaa tttttaaagg tatttggttg tcaataaaaa gaataagccg
    21421 ttgccatccc tggggcggta gtgggtatga ccctgtgcca taaaaaaacg gatcatattc
    21481 aattgatccg ttttaatcat tataattttt gcttattaag ctttctcaaa acgttcagct
    21541 actttattcc aatttacaac gttccagaaa gcggataaat aatcagctct tttattctgg
    21601 tatttcaggt aatatgcatg ctcccacaca tcaacaccga gaataggtgt gcctttaact
    21661 tctgccacat ccatcagcgg gttatcctgg ttaggagtag aggtaacctc caacttacca
    21721 tcttttacaa tcagccaggc ccagccactt ccaaaacggg tcataccagc agtggcaaac
    21781 ttttctttaa aggcatcaaa agatccgaat gtatcattga tagcttctgc cagtttaccc
    21841 gaaggagagc ctccggcatt tgcagccaaa ctttcccaga agaatgtatg gttccagtga
    21901 ccaccaccat tattacgaac agccggagaa attgatccgg caactgctac cagttcttcc
    21961 agtgatttgt tttcattcgg agtgccggtt acagctttat tcagattatc aacatatgcc
    22021 tggtggtgtt tgccatggtg tatctgcatg gtcagggtgt caatatgtgg ttccagcgca
    22081 tcgtgtgcat aaggaagtgg tgctaatgta aatgtcatga ttattatgtt taatgtttaa
    22141 agttgaatgt tatctcaaag gataacaaat ttattgcctt tatgttgttt gcagcttcct
    22201 ggtaaagaaa ttctttctga attcgaaaac tattttttta aacggtttaa tttcttcccc
    22261 ttttccatga aagtttcaac aatgcctctt gattcaagat ccagccggat agaaatggtg
    22321 taccagggta tggatttatt aaatcccggc atttttttgt ttataatttt tacaacatcg
    22381 tctgttaatt gggtaaaggt cttgccttta cttcctttca gactttgaag gacagcgtct
    22441 ttaaaaggga tataaatggc agcatcaatc cgcatttctc ttccactttt tgggtgttta
    22501 gcacttgatt tggttgcttt agtcatgggt taacgtttcc ctttaaagaa gtctttcatc
    22561 aattgggcac attcatcttt cattacgcct ctgattaatt cagttttggg atgaaacggc
    22621 cagttggcgc ctgctgtttt tttatatccg tttttttcat catcagcacc gtatacaatt
    22681 ttgccgatct tgctccagta aatagctccg gcacacataa aacaaggctc aatggttaca
    22741 tataaagtgg catcgggcag atatttactt ccaagtgaat taaatgcaga tgttaatgcg
    22801 atcatttcgg catgagccgt ggggtcgttc agtcgctcag tcatattatg ccctcttgaa
    22861 atgattcttt cattgacaac tacaacagca ccaatgggta tttcatcatc ttcaaaagct
    22921 attttagctt cttttaatgc ctgcgtcatg aagtattcgt cagtcatcgg gcaattggtt
    22981 tttgcataac ttcaaacaaa attacagtta atgtcagcag atataaccag gttggtgaaa
    23041 atgcggcaat attttgccag tggtgctaca aaaacatatt ccttcagaaa ggaacagtta
    23101 aagaagctaa agtcatccat tgccagtcat gaacaggata tttatacagc actatatgaa
    23161 gatcttaaaa aaagccctga agaaagctgg gttacagaaa atggattggt gattgctgaa
    23221 ctgaatactg cgatcaacaa tcttcaaaga tggatggaac ccgacagcgt aggcactaac
    23281 ctggtaaacc tgccatcggg gagcaaaata attaaagaac cattgggagt agtattgatt
    23341 attggtccct ggaattaccc ttttcagtta ttgatcaatc ccttaattgg tgcaatagcg
    23401 gccggtaatt gcgtggttct caaaccaagt gaatttgcac cggccactga tgccattatg
    23461 aaaagaatta ttgaagaagt gtttcccccg gaatatattt tatatgtaca gggagatggc
    23521 gcatcagttg ttccgggtat gatgaacaac tttacttttg atcatgtctt ttataccggc
    23581 agtaccacgg tgggtaaaat tatttacaaa atggccgccg agagattggt gcctgtaaca
    23641 ctggaactgg ggggtaaaag cccctgtgtg gtggaagccg atgccaatat cagggtggtg
    23701 gcacgccgta ttgccattgc taaattttcc aatgccgggc aaatgtgtgt ggcaccggat
    23761 tacctgctgg tgcaccattc ggtaaaagac aaagtattga gtgcactgaa ggaaacgata
    23821 caacaattct ttaccggaaa accagaagag agttataatt atggcaagat aattaatgag
    23881 aaacagttta cccgtatttg caattacatg caaaatggaa caattgcatt tgggggaaga
    23941 acaaatagag aaaagctctt tatagaaccc acgctattaa cggatgtttc cttaaatgct
    24001 ccggtgatga atgatgaaat atttggcccg gtattaccca tcatctcttt tcataccatg
    24061 gacgaggcga aagcagtaat agaccggcac ccggatccgc tggcctttta tatttattca
    24121 tctgacaata aaaaacaaca ggcatggctg gaagctgttc cagccggtgg tagttgtata
    24181 aacaattgca gctggcactt aaccaaccat aatttaccct ttgggggaag agggcacagc
    24241 ggcttgggct tttatcatgg caaatattca tttgaaacat tcagtcataa aaaggcggta
    24301 atgaaaacac ctacctggtt ggatccggac attaagtatc cgccgttcaa aggaaagttg
    24361 aaattgttta agtgggtaat taaataaata aatgaaaaaa cttaaaaagt ttgcaattgt
    24421 tttcttttta ttcattgcag catttgctgt ctatgtggaa atcgcaaacc gtaattccaa
    24481 aaatatgacg tacaggcaaa aaatattaaa ggctgtttac ccggtgttaa tgtgggcttc
    24541 tgggtttatc gggaagagta caaaaaagct ttcgaatgat acagttagac cgcctgttcc
    24601 tttttattcc ttaaaaggtg aatcaggcaa cggagatacc attgatttta actccttcca
    24661 gggcaaaaaa gtgttgcttg tcaatac
//
LOCUS       DAORTI010000045        24505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1616_length_24505_cov_8.413947, whole genome shotgun sequence.
ACCESSION   DAORTI010000045 DAORTI010000000
VERSION     DAORTI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24505
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1616_length_24505_cov_8.413947"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLO70_07790"
     CDS             complement(<1..521)
                     /locus_tag="PLO70_07790"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HQZ74403.1"
                     /translation="MSSESIDKATQTQIANIEKNTGKKLAEWIAIVNKSGFTKHGELV
                     NFLKEKHGFTHGNANTIVHFAKQSHAGAAENSDDLITEQYKGKENLKPWYDKIMAEIN
                     KFGKDIEVAPKKSYVSLRRKKQFAILQPSTKDRLDVGLNIKGVEPSGNVEAGAKWNAM
                     CTHRIRVEEEKTI"
     gene            complement(603..1073)
                     /locus_tag="PLO70_07795"
     CDS             complement(603..1073)
                     /locus_tag="PLO70_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQZ74404.1"
                     /translation="MQSKATTVDAYLAELPKDRQKAIAKLRSVIKKNLPKGFKEGMGY
                     GMMGWSVPHSIYPAGYHCNPKDPLPFIGLASQKNFIAVYHMGVYANPKLLKWFTEAHA
                     KASPKKLDMGKSCMRYKRAEDIPYELIGELASKITPEEWIKTYESVFKDNRKKK"
     gene            complement(1075..1602)
                     /locus_tag="PLO70_07800"
     CDS             complement(1075..1602)
                     /locus_tag="PLO70_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQZ74405.1"
                     /translation="MIILYIILGIIAVLLITGLFIPKGMQATREIIINKPNADVFNYI
                     KQLKNQDNYSKWGSMDPNMKKEYRGIDSTVGFVSAWEGNKKVGAGEQEITGIEEGRKL
                     STELRFIKPFKSVAQSAMTTEALSDNSTKVSWGFEGQMNYPMNVMKLFMNMEKAIGND
                     FSTGLSNLKIILEKQ"
     gene            complement(1615..2013)
                     /locus_tag="PLO70_07805"
     CDS             complement(1615..2013)
                     /locus_tag="PLO70_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQZ74406.1"
                     /translation="MDNAISWFEIPATDIDRAQKFYETIFQIQMQPMDFQQTKMRMFP
                     LDDPMKGIGGTLIDSGGFHKPSATDGPLLYLNGNPDVQITLDRVEAAGGKIVVPKTEI
                     SPEYGYMAVFIDTEGNRIALHSVPEKYLQK"
     gene            complement(2032..2460)
                     /locus_tag="PLO70_07810"
     CDS             complement(2032..2460)
                     /locus_tag="PLO70_07810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQZ74407.1"
                     /translation="MATELKTKKNDANVEAFLQTIENEEKRKDCLTILSMMKKITREE
                     PKMWGSSIVGFGTYHYKSERSKREGDWFITGFSPRKQNLTIYIMPGVYYYKELVQQLG
                     KCKTSVSCLYINKLKDVDTVVLKELINTAYKRMKEIANKK"
     gene            complement(2638..3747)
                     /locus_tag="PLO70_07815"
     CDS             complement(2638..3747)
                     /locus_tag="PLO70_07815"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ74408.1"
                     /translation="MKRLYFFCTLAIVTINLSAQMDIPPVGGNPRAMIAEELGITSIT
                     IHYGRPDVNKREGKIFGDGNLVPYGFSTTNFLTSKNTSPWRAGANENTTITFEHDVKV
                     EGRDIKAGTYGLHMALAADMVTLIFSNQNDAWGSFYYEEKNDALRVNVKPVALDKNVE
                     WLKYEFIEHKEKYCVIAMQWEKLSVPFKIEMDVDNIVIARLRQQVTSQKGFNSNNMLQ
                     AAQYCLNKNINLEEALAWSIRAINGFQGQKSFITLRNLATAYEKLNRIPQADSTMDEA
                     LLIATANQYTAYGRQLIGQKRTDKAMEVLKASEKRYGDMFGVNNGLMSVYSAKGDFKN
                     AIKYAEKALAQAPNENSKKQIEGFLVKLKEGKDIN"
     gene            complement(3980..4495)
                     /locus_tag="PLO70_07820"
     CDS             complement(3980..4495)
                     /locus_tag="PLO70_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74409.1"
                     /translation="MKKLLLFAACVFFSSVVFSQKPCSRPEYRQFDFWIGEWEAFDLK
                     GNKAGDSKISLILDSCIILEEWTSASINQGIRYAGKSFNTYNAATKQWQQTWVDNVGG
                     SNEYLLGKFENNKIIYRSSLFPFNKDTMAIRKMTFTNLNPDKLRQHGEISKDNGTSWT
                     TEYDLEYRRRK"
     gene            complement(4648..5046)
                     /locus_tag="PLO70_07825"
     CDS             complement(4648..5046)
                     /locus_tag="PLO70_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74410.1"
                     /translation="MKKISLLLVASLVLTAVSFAQPGGGAPRVTAEERVARIHQKIDS
                     AFKLESTKLANLDTALTVLYKAQDAKRQELMAGGMPDRETFMAEMKKYTDAQDEILKA
                     VLTEEQFTIWKEKIQPSMRPQRTPGGGRGK"
     gene            5195..5731
                     /locus_tag="PLO70_07830"
     CDS             5195..5731
                     /locus_tag="PLO70_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HQZ74411.1"
                     /translation="MEYSKLVAVTGLPGLFELINSKTDGAIVRSLEDKSTRFVSSRVH
                     NFSHLESIEVYTIRDNVNLVDVFNAMDKAGGKLPDEKDAAAVKKYFEKAYPDMDFERV
                     YASDMKKMVKWFEVLKKNNVEIKLSEIPEEAEEEITEPVAEEKPAKKTPAKKKEASTA
                     EAKAEEAPKKTTRKKKAE"
     gene            5815..7533
                     /locus_tag="PLO70_07835"
     CDS             5815..7533
                     /locus_tag="PLO70_07835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HQZ74412.1"
                     /translation="MTYSADIKKLTRHFLPNNFIITNWESLEPYFKNLDERVINSVAD
                     LEQWLKDASEVEAIISEDACWRQIKMTCDTENKELEESFTFFMMEIQPKIQPYADKLN
                     KKLVSCPFTKELDKQKYFTYLRNVKKNIDLFREENIPINAELNVMQQQFGVISGKMTV
                     EVKGQEYTLQQAAKFLEDEDRSLREEVYRKISNRRLQDKKPLSDLFSSLLQKRHQVAV
                     NAGFENYRDFRFLELGRFDYTKESCYQFHEAVKLYVMPLVNQLYEAKKKKLCLDTLRP
                     WDVEAEPAGTQPLRPFKTGVELTEKTIACFNELRSFFGECLRRMSEMGHLDLESRKGK
                     APGGYNCPLAESGAPFIFMNAAGTLDDVTTMVHEGGHAIHSFLAHELELTGFKEYPTE
                     IAEVASMAMELFSMDHWQVFFDSTAELKRAKEQQLERVITIFPWIATIDKFQHWVYEN
                     PNHTEEERADRWFEILKEFSSPVIDFTGLDEYRRFAWHRQLHLFEVPFYYIEYGIAQL
                     GAIGLWQQYKNNPESAINNYLKALELGGTKTLPELFRAAGLQFDFSPAYISQLMGFVK
                     KEMDSL"
     gene            complement(7730..8170)
                     /locus_tag="PLO70_07840"
     CDS             complement(7730..8170)
                     /locus_tag="PLO70_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQZ74413.1"
                     /translation="MPKQASVNIENKIAGLLKRNNLSITDSRKKILHLFLEQTGALAH
                     GDIEKRAGEKFDRVTVYRTLQTFVDKGIIHTIPTADNSIRYALCKDECAGGHHHDHHI
                     HFVCHKCNNTYCLDDVVTPEIKLPKGYSAEHVEVVVDGICKNCR"
     gene            complement(8187..8813)
                     /locus_tag="PLO70_07845"
     CDS             complement(8187..8813)
                     /locus_tag="PLO70_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQZ74414.1"
                     /translation="MSKKGWLYTCFFIVLAVGFYFVLARVIPGFTKIGVAPVSFVRPF
                     AFTTQDGKPFTEKDVAGKVYVAEYFFTTCKGICPKMNNNLKLVYEKLKSEKDFLILSH
                     TCDPETDSVPQMKKYADSMRVNTQQWVFLTGRKDSLYTAARISYTIDDPVNNLKSIED
                     DFLHTQFWALVDRNGDVRKIYDGLKESEVKELIKDAEKLLKDIPGQKN"
     gene            8918..9310
                     /locus_tag="PLO70_07850"
     CDS             8918..9310
                     /locus_tag="PLO70_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HQZ74415.1"
                     /translation="MKFHINWDALGIGASLACAIHCALLPLFLSSLPLFGINIIHHLG
                     FEIGMILLALAIGSYSMYHGFKKHHHSLLPLSLFYAGFIFLVLKQFFLHYSTWLLIPA
                     VILIVTGHLLNYKSCRVHNHAHADDCNH"
     gene            9372..9794
                     /locus_tag="PLO70_07855"
     CDS             9372..9794
                     /locus_tag="PLO70_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HQZ74416.1"
                     /translation="MKYFLVLLSFCSVMGSQAQSKNETTIRNILNEQTGAWNKGDING
                     FMKGYWENDSLMFIGKTGITYGWTNTLNNYKKGYPDTAAMGKLTFTLFSVKRLSKRHY
                     HVVGKWFLKRSIGDVGGHYTLLFQKINGRWVIIADHSS"
     gene            9844..10746
                     /locus_tag="PLO70_07860"
     CDS             9844..10746
                     /locus_tag="PLO70_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HQZ74417.1"
                     /translation="MAFIILGILALIAGIVLQKNEQVAPFAKVARLVAVLLIIVGVLT
                     KSVVQINAGQIGVKTLFGKVQNDVLHSGLHFINPLMEVLSLDIKTQNYTMSGVQDEGN
                     RSADDAIRVLTADGLEVTIDLTVLYKLLPSEAPKLVRETGEDYKDKIVRPLTRTKIRD
                     NSVYYDAISLYSSKRDEFQQRIFKSIEDDFKKRGLILEQLLVRNITLPLSVKATIEQK
                     INAEQDAQKMQFVLLKEKQEAERKRVEAQGIADYQRIINIGLTDQQLQYEQIKAYLEL
                     AKSPNSKVIIMGKGNAPVILDTKN"
     gene            complement(10743..11153)
                     /locus_tag="PLO70_07865"
     CDS             complement(10743..11153)
                     /locus_tag="PLO70_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74418.1"
                     /translation="MELKQISKWSVITGAIIILGIKILIRPLYHFDEPMHFFLGIAPN
                     LIGSFLIPFGACWFFSGKDFLLARMFRIECSYDLRFVCLLGFGMLVVNEYLQLIPVFG
                     RTFDPYDILFSGFGLITSYFVFGRLQQRYEEAPV"
     gene            complement(11515..12288)
                     /locus_tag="PLO70_07870"
     CDS             complement(11515..12288)
                     /locus_tag="PLO70_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HQZ74419.1"
                     /translation="MPSAKQKKKIRRSVFRVLAVVFVGTAAWMLYQWLTFRKIKYTRY
                     PEFGIAIPGGYSIHGIDVSKYQHTIAWEGVKKMKVKEIQLGFAFIKATEGISNTDPQF
                     RRNWRKAKQAGVIRGAYHFFIASRDGKAQAENFIKKVELEAGDFPPVLDVEQLNGTGT
                     LQLQKEAREWLDLIESHYGVKPFIYTNVDFYNRHLGSQFDEYPLWVAHYYQPEQPRIR
                     RGWVFWQHSDRGRVNGIISPVDFNVFNGDSAAFKRILIR"
     gene            12440..13333
                     /locus_tag="PLO70_07875"
     CDS             12440..13333
                     /locus_tag="PLO70_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HQZ74420.1"
                     /translation="MPRLLIIGGNGVLGSAAANHFLENGFRVTVFVRNRSRAVSLEKK
                     GAIIVEGDLAKPSTLTGIFTGIDIVLTAAHGMLGMGNNKSEKVDGTGHKSLIEEAQKS
                     GVKHFIYTSVFTASADHPVDFFRTKYKMERLITKSGLNYTILQLPAFMEWHVYNLLGK
                     NIVKKGKTTILGRGNTPVNFIAVKDVVAAIDKIILKEEFYNKTIPVAGPQNMTRNEIA
                     DLFGKALNIKPKVGHAPVRLLKLFSVLVSPFHPGIARVMKLSIYTENSDETMDPKYSV
                     QQFGLSPTTIEEFIQSVTTKR"
     gene            13541..14869
                     /locus_tag="PLO70_07880"
     CDS             13541..14869
                     /locus_tag="PLO70_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ74421.1"
                     /translation="MSFVIEQFYLFFQGALVFQSVFFFLIWAATRRNDVLWYSLYLLT
                     AALYFFINATGTFFKIEEAAVFESSWYSLLNIPIIIIENLFYLLFIKAFFYDIIASQK
                     VKNILRLTLYSVPVLFFCFIIFRVTGISTYNLFYIVKLLSVIPAIFIAYIVIKNKLPY
                     ATLVANGLIFTITGTLVTVIMIILRNNDVHTMFTDYYPLLFIRLGILGDMFFYQFALL
                     KKWRWQEKELALQQHQTKTEVERVRNEISRELHDDIGSTLSGINMYSHMAMTQSQSGD
                     SKATVQSLQTIQQSAAEMVGKLKDIVWANNLKDGDTDAFFETLKDYVLMITKPAGIVP
                     EFNFSATAETNRLSKEEKYHIYMICKEAVNNMVKHSGADSLAVSFEKKDTGLDITIAD
                     NGYGLKPGKNNAGNGLNNMRKRAEEIGAIFSLQSGATEGSKISLYLKITR"
     gene            14909..15559
                     /locus_tag="PLO70_07885"
     CDS             14909..15559
                     /locus_tag="PLO70_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74422.1"
                     /translation="MAVRVTIFEDSKLIREALEAIINGTPGYTCCGAFSSGNNWEQDI
                     NRSQPDVILMDIEMPGMDGIQLTRCITQKFANIKVLIQTVFDENNKIFNALCAGASGY
                     ILKTDPPHKYLEAISEVNNGGSFMNAAVARKTLNFFAQKNIILVTPEGTDYQLSKREK
                     EVLMELIDGNPLKNIAARLFISLETVRTHVKHIYEKLHVTNRTEAIMKATQQGLQK"
     gene            15575..16153
                     /locus_tag="PLO70_07890"
     CDS             15575..16153
                     /locus_tag="PLO70_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74423.1"
                     /translation="MNYAVLITAILILSCSNPSVKQERKTNDAVLDEQKEQAAIIKVI
                     EKETECFFQRDYNCWQSCFAQTDYAFQAWNNENGTFDAYSGWEAVSNHAAIYLKNNPV
                     AKNKDMGQEYSAKEIRSSHPKVIRKNMKIKFFSDKLAYLVWDQYNSDTFSQRFTWSKD
                     SRLMEKIGGEWKIVNVTSYWDFKNPIPADSLQ"
     gene            16218..17069
                     /locus_tag="PLO70_07895"
     CDS             16218..17069
                     /locus_tag="PLO70_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74424.1"
                     /translation="MCKLLLLITTTFCILQSHAQNVSIGTNNTSNAKLTVEGAVGPVV
                     GLFKNGSGISLSVSSNNENVGLNYADGKAIATGIGGRLLLNNSASTGGIGLQYYSSTT
                     AGATFPTAINVLRHTDNRWAGSGHWIDMLYGQPGRVRTTNQLYTEATGEFNLVPIGII
                     YFKVTNMHLGSNSTVVIKNAGNGATMSNSILQFVCDPGSPIDVGIVSFILNLDWITKE
                     YDDVYVVGVPSFASFTAVTYTSAADFVRRPANQQDWLEVTYMASRLYSDSQFSGTLML
                     YGNRTLN"
     gene            17088..17621
                     /locus_tag="PLO70_07900"
     CDS             17088..17621
                     /locus_tag="PLO70_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74425.1"
                     /translation="MKIITALVLYISCALFISSCSDSKTKAQPEEQKEKATEKQTISI
                     ITEKHVKIPNSSLYIIPPAGFAADETSGTLAQTEGTAHYMQMQIFSGETQESLFAKFK
                     SEADKNFPGSWKEEPITINGHAATICHCKSAGYSQYFMAFTDGYTDEMIIANYEEKDA
                     ATAKAMYEALKTVVVKK"
     gene            17633..18127
                     /locus_tag="PLO70_07905"
     CDS             17633..18127
                     /locus_tag="PLO70_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74426.1"
                     /translation="MKILFYTFLLAGIILACKNSKAEKQSDGNNKTESKADTARMASF
                     PGTAIYIQLPKGFVWNETAMGFYKEEDGSVIKYNEFKKMRYAANMSAEETMGSLINQQ
                     PITINGYKGLIKTYQQGSTGIQLELSFGDASFMEFVEATYFSHQEQTAKDILAALKTI
                     QVKK"
     gene            18154..18615
                     /locus_tag="PLO70_07910"
     CDS             18154..18615
                     /locus_tag="PLO70_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74427.1"
                     /translation="MKAIQFFISAILFCTAAFTQSPSCSNVIKEDSVRFSRKSVFLNW
                     AAKKKLYHIAMELNRNPDCRIVVTGYGNSCLICQQTSWDRSYSVVQYLRQGGVDSLRF
                     VFRYAQEAYSPSVVSIRNSLPGEDGPALDEPPSPCYSYHGFHKKRCRGKRL"
     gene            18744..19748
                     /locus_tag="PLO70_07915"
     CDS             18744..19748
                     /locus_tag="PLO70_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74428.1"
                     /translation="MLRHFLLFISASFITATSLAQTYGIVKFNNVPFTNGSEAGKTSF
                     KSNEFIYARVELEKSVKEYFKITNPDGSSKPHVLIFKFSKRYTEVDGKNYSDNFTNGE
                     YLYIKPEDLSKTYIDLDILPDPAMANDVFCLVSNFQGGMYTSPIYRVRPKGYKNNSTI
                     NYSIQLVGGTTVANNSGNENLPKVTGTFDLLVNHSDEPLLKQNSDLADKKVQANGLNL
                     SSLPTIFSRPFKTTDPKLTAAKLTAILKRDYPHRKVLKMALDSDGGQLWLVSKNDFGI
                     PRYRYFNGLLHVAYMEDGVCKVGSVELIENYLGGGKYAALAADYWSEYDRTINCTAVK
                     "
     gene            19822..20460
                     /locus_tag="PLO70_07920"
     CDS             19822..20460
                     /locus_tag="PLO70_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74429.1"
                     /translation="MKFQLFLCVTLIASQLLYGQLPTGPDGKTLYQKGDCKTILPNVK
                     YKCVFCEDKELTKNCKEYDCSLTECKESKSTKGSNGRMQAKPISIDGKQVRMRDDSDT
                     TSKLPKGTKFENGKVVITNGYKAVQSSDKTMVFILADNGNNVEGSFSCKCFLGLGACG
                     VIISGSAVGCSGAECCRFKVTTGNLSGLTMQDIERAPEKLKWKNLVLPVKSN"
     gene            20471..21607
                     /locus_tag="PLO70_07925"
     CDS             20471..21607
                     /locus_tag="PLO70_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74430.1"
                     /translation="MKKYLICILLIVFYNRIDAQTVTVIDHRGGKDTVKPTTVIDHRI
                     VRDMSPGLVKPVFEEMPVYRIQLRITTSDNNGTDDAVWVELNEEGHRFYLAKGIDNFQ
                     PRKTVIYDVVDADIKKLKDIRFLKFGVKGEDGPCFKKIELIVNNSGTPIYTTQIVSNA
                     GTCFDNNNSQVAENFIVTYNNLRNHPYWNYNGARANIWRPVKKINKEWVVSLIEACIG
                     NQMVQEGSKLKWGSHGGVFENNTLFGDAVEVKYKNNHTLSVDLDLERSITGSNPEANI
                     KFELDFRCNDGVINIETQNVTVSADGVGAVQDFIRTTGFDLVSIAIGLFAAPAAATPL
                     TVGAWFALRRAIAFSIKLNPATPNTSNSCTQTIVTEVGDILVKK"
     gene            21629..22291
                     /locus_tag="PLO70_07930"
     CDS             21629..22291
                     /locus_tag="PLO70_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74431.1"
                     /translation="MKIKSLLKAITVFLFILPFCTSISAQDAQIDGKSYETVRKVNLN
                     ADAEFVKLKQKSAGTASGESYIKVNNEYAKTSFTAAALKNFFATGKLSSLGTVKKTIL
                     AGNPVMYIDNGSSIAIVNYNKESIGFIEIQNPKQIPTVRNHLAKAGNNDDPGGSTGDP
                     LLDCGVVCLNTFIACGNSAVCRSAYKKCWSDCKNRHQSQGGSGGTPSYYIVSLNALAV
                     KF"
     gene            22294..23265
                     /locus_tag="PLO70_07935"
     CDS             22294..23265
                     /locus_tag="PLO70_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74432.1"
                     /translation="MQVNTKIAALFLYCLAFFISCKSTDKKDNEEGKISAFVKSTAHT
                     NIPGTHIFMIPPSGFKASTGSMKGFIKDETTTFLSVTEFKGKSFVAEAANNSAEKMKA
                     KGFSIFMEEETTVGPYKARRMRIGDGETSTVWQLLFGDDSFAVMIMGIYPPHDEISGK
                     QIAEAIETVVYDKDMVVDEMAGMNYTIKSNESRYKFLKIQAGLAFYSLEGNADLKNDD
                     IPFLVLSQVPRENASAESFAETALESTLRNGFSNPSIKSKGFLTINNNSAYEMEVACM
                     LKGKPVILYIAVVTKDRTAVMVQGVAKKNIAETITGFKDFADAIVIK"
     gene            complement(23268..>24505)
                     /locus_tag="PLO70_07940"
     CDS             complement(23268..>24505)
                     /locus_tag="PLO70_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQZ74433.1"
                     /translation="AYTIYGGISFYQRKEIKDFVAYLRIIVNPSDEEALKRIINYPAR
                     GIGKTTVDKLVLSANENNVSMWQVLEKANEFGFRAGTFTALEEFVIMIKSFASMLTKQ
                     NAYEVAFHVGKQTNLVKELFNDKSTEGVQRYENIQELLNSIKEWTESPDNEDGELVDK
                     SLASYLQQITLLTDADEKDPDADTVKLMTIHAAKGLEFSCVFAAGLEEMLFPNAMAIN
                     TREELEEERRLFYVVVTRAKQRLWITYANTRYRFGQLVQNEPSRFIEEIPEQSMDRSF
                     AGGGAKNQVSGSKWSKGSAFDRMKARNVDDGESGSARQQSGTYYNSSPAASKYAPSYL
                     PPKGVPPKVKEHVPSADFEASDTSNLQAGQKVEHQKFGFGEVLKMEGAAHNPIATVKF
                     EHNGEKKIMLNYAKLRIVE"
BASE COUNT         7423 a         4912 c         4892 g         7278 t
ORIGIN      
        1 ctgattgtct tttcctcttc tacacgaatg cggtgtgtac acatggcatt ccattttgca
       61 cctgcctcca cattacctga aggttctact ccttttatat tcagcccaac atccagccgg
      121 tctttggtag atggctgaag gatggcaaac tgttttttcc tcctcaaact gacataggat
      181 tttttaggtg ccacttctat atcttttcca aacttgttta tctccgccat tatcttatca
      241 taccagggct tcaggttttc tttcccttta tattgttctg taatcaggtc atcgctgttc
      301 tctgcagcgc ctgcatggct ttgtttggca aaatgcacta ttgtattggc attgccatgt
      361 gtaaacccat gcttttcttt caaaaaattt accaattctc cgtgtttggt aaaaccactt
      421 ttgttgacta tggcaatcca ttctgccagt tttttaccag tatttttttc aatattggct
      481 atctgtgttt gcgtggcctt atcaatagat tcactgctca taagtgcttg tttaatttag
      541 tatccattta gttgcattta tatacagcat tgccataagg ataaaccttt gcctgcatta
      601 ttttattttt tcttccggtt atctttgaaa actgactcat acgttttaat ccattcttca
      661 ggagtgattt ttgacgccaa ctcaccaatc aattcgtatg gaatgtcttc ggctctttta
      721 taccgcatac agcttttacc catatctaat tttttaggtg atgctttcgc atgggcttct
      781 gtaaaccatt tcaggagttt ggggttggcg tatacaccca tatgataaac agcaataaaa
      841 tttttctgtg aagcaagccc tataaatggc agcgggtctt ttgggttaca atgatacccg
      901 gcaggataaa tgctatgtgg aacactccag cccatcatac cataacccat tccttcttta
      961 aaccctttag gaaggttttt cttaattacc gaacggagtt ttgcgattgc tttttgccgg
     1021 tccttcggca attccgccag ataggcatct actgttgttg ctttagattg catactattg
     1081 cttttcgagt attattttga gattgctgag gccggtgctg aaatcattcc cgatagcttt
     1141 ttccatattc atgaacagtt tcatcacatt catcgggtaa ttcatctggc cttcaaaacc
     1201 ccagcttact ttggtgctgt tatcacttaa cgcttctgtg gtcatggcgg actgagcaac
     1261 tgatttaaag ggttttataa agcgtagttc tgtacttagt ttccgacctt cttcaatgcc
     1321 ggttatttcc tgttcgccgg caccaacttt tttatttcct tcccatgcac ttacaaagcc
     1381 taccgtcgaa tctatgcccc ggtactcttt tttcatatta gggtccatac tgccccactt
     1441 actatagttg tcctggtttt ttaactgttt gatgtagtta aacacatcgg cattaggctt
     1501 attaattata atctcccggg tggcttgcat accttttggg ataaacagac cggttattaa
     1561 taaaacggca attatgccga gtatgatgta tagtattatc atgtgtgttt gattttactt
     1621 ttgcaggtat ttttctggca ctgaatgcag ggcaatccgg tttccttctg tatcaatgaa
     1681 aacggccata tacccatatt ccgggctgat ctctgtttta ggaactacta ttttcccccc
     1741 ggccgcttct accctgtcaa gcgttatttg cacatccgga ttaccattga gatagagcag
     1801 cggaccatct gtagcagatg gtttatgaaa cccgccactg tctatcaatg ttccgccaat
     1861 gcctttcatc gggtcatcaa gaggaaacat tctcattttt gtttgctgaa aatccatggg
     1921 ctgcatctgt atctggaaaa tagtttcgta aaatttttgc gccctgtcga tgtcggttgc
     1981 cggtatctca aaccagctga tagcattatc catgttgatt gattttgttg gttatttttt
     2041 atttgctatt tctttcatcc gcttatatgc cgtattgatc agctctttca aaacgacagt
     2101 atccacatcc ttcagcttat taatgtagag acaggaaaca ctggtcttac atttgcccaa
     2161 ttgttgaacc agttctttat aataataaac tccgggcatt atgtatatgg tgagattttg
     2221 ctttctcggt gaaaaacctg taataaacca atcaccttcc cgtttgcttc tttcagactt
     2281 ataatgatag gtaccaaatc ctacaatgct gctgccccac atctttggtt cttccctggt
     2341 gatctttttc atcatggaga gaatagtcag acaatctttt ctcttttcct cattttcaat
     2401 cgtttgaaga aaagcctcca cattggcatc attctttttt gtttttaact cagtagccat
     2461 taatctattt gtttattaat atgatcagtg taattattga ttccttaatt acagtttatc
     2521 cttccctttt gcccattttt atttcaattt tgattcagca cctggttggg tagctatacc
     2581 acttatatac gccatgctaa taaatccggg ttggcaagag ccatataaaa ggctctttta
     2641 attaatatct ttcccttctt tcagttttac tagaaaccct tctatctgct tttttgagtt
     2701 ttcattcggg gcttgtgcca aagctttctc tgcatacttt atggcatttt taaaatctcc
     2761 tttggctgag tagacgctca tcaagccatt attcacacca aacatatcgc catatctttt
     2821 ctcactggct tttaaaacct ccatagcttt atctgtcctt ttttgcccga tcagctgacg
     2881 gccataagcg gtatattgat tagctgtagc aatcagtaat gcttcatcca tcgtagaatc
     2941 tgcctgcggg atacggttta atttttcgta agccgtggcc aggttacgaa gtgtgataaa
     3001 acttttttga ccctgaaatc cgttgatagc cctgatgctc cacgccaacg cctcttctaa
     3061 gttaatattc ttatttaaac aatactgggc tgcctgcagc atattattac tgttaaaccc
     3121 tttctggctg gtaacctgct gcctcagtct tgcaattaca atattgtcaa catccatttc
     3181 aattttaaaa ggtactgaca gtttttccca ttgcatggct attacacaat atttttcttt
     3241 gtgctcaata aactcatact taagccattc cacattttta tccaatgcga cgggcttaac
     3301 atttacccgt aaagcatcat ttttttcttc gtagtaaaaa ctgccccagg catcgttttg
     3361 attggaaaag ataagcgtta ccatatctgc tgccagtgcc atgtgcagac cataagtgcc
     3421 ggctttaatg tctcttcctt caactttcac atcatgctca aaagtaatgg tcgtattctc
     3481 attggcaccg gcccgccaag gtgatgtgtt cttactggta aggaaattgg tcgtactgaa
     3541 accataggga accaggttac catctccgaa aattttaccc tcccgtttgt ttacatcggg
     3601 gcggccgtaa tgtattgtga tgctggtgat gcccaattct tcagcgatca tggccctggg
     3661 atttcccccg actggaggta tatccatttg ggctgaaaga ttaatggtaa caatggccaa
     3721 tgtgcagaaa aaatagagtc ttttcatata gcagctttgg tgatgaatag aaattgcaga
     3781 gttggattct aaagttcata taataaatcc aaaatatctt tccactcatc atttcttctg
     3841 cctgtcagcc aacttacctt ttacagtgat tcctgtactg cgcctgctga taaaatgacc
     3901 tggttaaaat gaattcattg cattaaaaac atccgtttgc gttcttgttt ttcagtacgt
     3961 ttaagaactt taataaattt tattttctcc ttctgtattc caggtcgtat tccgtagtcc
     4021 agctggtacc gttatctttt gaaatttcac catgttggcg gagcttatcg ggatttaaat
     4081 tggtaaatgt cattttgcgt atggccatcg tgtctttgtt gaaagggaac agtgaagacc
     4141 tgtaaatgat cttattgttt tcaaattttc ccaacaggta ttcattactg ccaccaacat
     4201 tgtccaccca tgtttgctgc cattgttttg ttgccgcatt gtatgtattg aagcttttac
     4261 ctgcatagcg gattccctgg ttgatactgg cactggtcca ctcttctaaa ataatacaac
     4321 tgtccaaaat caggctgatc ttactatctc ccgccttatt accttttaaa tcaaatgctt
     4381 cccattcccc gatccagaaa tcaaactgtc ggtattcagg acggctgcat ggcttttgag
     4441 aaaaaaccac tgaactaaaa aatacgcagg ctgcaaacag taatagtttt ttcatgttag
     4501 tttttttaaa cagacagccc gttacaaaga ttagctgaat catttatccc tgtttatcac
     4561 caactataaa actaatacaa ccaaaaaaat aaaaaccgcc ccgcaaaatg cgaagcggct
     4621 tttagtatag cccgaaattc atcctaatta tttacctcga ccgccgccgg gtgttctctg
     4681 cggacgcata gatggttgta ttttctcttt ccagatggta aactgttcct cggtaagtac
     4741 cgctttcagt atttcgtcct gggcatcagt atattttttc atttcggcca taaaggtttc
     4801 cctgtcaggc attcctccgg ccattaactc ctgcctttta gcatcctgtg ccttgtacag
     4861 aacagttaat gcagtgtcca gattagctaa ttttgtggac tccagtttaa aagcactgtc
     4921 gattttttga tgaattctgg ccaccctttc ctcggccgtc acccggggcg cacccccgcc
     4981 tggttgagca aaagagacgg ctgtcaggac taaagatgct actaataata aagaaatttt
     5041 tttcattgat gataaatttt gaagtaatat ggaaataacc ggtaaatatg acggttcttt
     5101 ctcctaaacc gttcgctaac cggaaactta ttttcaaagc tactcatttg acagaactca
     5161 agtatcttgc agtcccttaa ttattattaa tcttatggag tacagtaaat tagttgccgt
     5221 aaccgggttg ccggggttat ttgaattgat caacagtaaa actgacggag ccattgtccg
     5281 ttcgctggaa gataaaagca cccggtttgt gtccagccgt gtacacaatt tctctcacct
     5341 ggaaagtata gaagtgtaca ccatccgtga taatgtgaac ctggtagatg tatttaacgc
     5401 catggacaaa gctggtggaa aattacctga cgagaaagat gccgcagccg taaaaaagta
     5461 ttttgaaaaa gcataccctg atatggactt tgaaagagtg tatgccagtg atatgaaaaa
     5521 aatggtaaaa tggtttgagg tgctgaagaa gaataatgtg gagataaagc tttctgaaat
     5581 tccggaagaa gctgaggaag aaattactga acctgtagct gaagaaaaac cggcaaagaa
     5641 aacccctgca aaaaagaaag aagcatcaac cgccgaggct aaagcagaag aagcaccaaa
     5701 gaaaactaca agaaagaaga aagcggaata gaaaaattgc gggttgtttg ttatgagctg
     5761 cgagctattt caattatagt tgaaatagct tttttcaatt taaaatgaat atgtatgacc
     5821 tacagtgctg atattaaaaa actgacaaga cattttttac caaacaattt tattatcact
     5881 aattgggaga gcctggaacc ttattttaaa aaccttgacg aacgggtaat taattctgtg
     5941 gctgaccttg aacaatggtt gaaagatgcc agtgaagtgg aagcgataat aagtgaagac
     6001 gcctgctggc gacagatcaa aatgacctgt gataccgaga ataaagaact ggaagagtcg
     6061 ttcacttttt ttatgatgga aatccagcca aagatccagc cttatgcgga caagttgaat
     6121 aagaaactgg ttagttgccc ttttactaaa gagcttgaca agcaaaaata ttttacgtac
     6181 ctgcgtaatg ttaaaaagaa tattgatctt ttccgggaag agaatatacc gatcaatgct
     6241 gaattgaatg tgatgcaaca gcaattcggt gttatctccg ggaaaatgac cgtagaagta
     6301 aaaggccagg aatatacatt acaacaagcg gcaaaattcc tggaagatga agatcgtagc
     6361 ctgcgggaag aagtatatcg taaaataagc aacaggagat tgcaggataa aaaaccatta
     6421 agtgatttat tcagttccct tttgcaaaag cgtcaccagg tagcggtaaa tgcaggattt
     6481 gaaaattacc gggactttcg ttttcttgaa ttaggccggt ttgactatac taaagaaagt
     6541 tgctaccagt ttcatgaagc ggtaaaacta tatgtgatgc cgttggtgaa ccagctttat
     6601 gaagctaaaa agaaaaaact gtgtcttgat acacttcgac catgggatgt ggaagcagaa
     6661 ccagccggca cccagccctt acggcctttt aaaactggtg tggaactgac ggaaaaaaca
     6721 attgcctgct tcaatgaact ccgttccttt tttggtgaat gcctgagaag aatgagtgaa
     6781 atgggacatc ttgacctgga aagccgtaaa ggcaaagcac ccggcggcta taattgtccg
     6841 cttgcagaga gcggcgcacc ttttatcttc atgaatgccg ccggaacact ggatgatgtg
     6901 accacaatgg tacatgaggg cgggcatgcc atccattcgt tccttgcgca tgagctggaa
     6961 ctgactggtt ttaaagagta cccgactgaa attgctgaag tagcaagtat ggccatggag
     7021 cttttcagta tggaccactg gcaggtattt tttgatagta cagctgaatt aaaaagagct
     7081 aaggaacaac agcttgaaag agttattacc attttcccct ggatcgccac aatagataaa
     7141 ttccagcatt gggtatatga gaacccaaat catacggagg aagaaagagc tgatcgctgg
     7201 tttgaaatac taaaagaatt ttcctcgcct gttattgatt tcaccggact tgatgaatat
     7261 cgccggtttg catggcatcg ccagcttcac ttatttgaag tgccctttta ctatattgag
     7321 tacggcattg cgcaattagg tgcaattggt ttgtggcagc agtacaaaaa caacccggaa
     7381 tccgccatta ataattacct gaaagccctt gagttgggag gaacaaagac attaccggag
     7441 ctattcagag cggcaggttt acaatttgat ttctcccctg catatatcag ccagttgatg
     7501 ggatttgtta aaaaagagat ggatagcctg tagaaataaa aaatacccta aaagtggtat
     7561 gccctttttt cggaaatatg aaactgacat catacatttg aatcagaaaa aataggtgct
     7621 tcattgcact attacatagc tactaatcag agccttaacc taaagtcccg atgtttctac
     7681 accggggctt tgttttttac gccttttatg atatgacctg gcagattttt taccggcaat
     7741 ttttacaaat tccatccact acaacttcca catgttcagc cgaatagcct ttgggaagct
     7801 ttatttccgg ggtaaccacg tcatccaggc agtaggtgtt attgcattta tgacacacaa
     7861 agtgaatgtg gtggtcatga tgatggcctc cggcacattc atctttacat aaagcatagc
     7921 ggatcgaatt atcggcggta ggaatcgtat gaatgatccc tttatcaaca aaagtttgca
     7981 gggtgcgata gactgttacc cggtcaaatt tttctccggc ccttttctca atatctccat
     8041 gggccagtgc tccagtttgc tcaaggaaaa gatgcagaat ctttttccgg ctatcagtaa
     8101 tgctcaggtt attgcgcttc agtagtcccg caatcttgtt ttcaatattt acacttgctt
     8161 gtttaggcat tatataataa agatatttaa ttcttttgcc cgggaatatc cttcagtaat
     8221 ttttcggcat cctttattaa ttcttttacc tcactttctt tcaacccatc ataaatctta
     8281 cgaacgtcac cattcctgtc taccaatgcc caaaactggg tatgtaaaaa atcatcttct
     8341 atgcttttca ggttatttac cgggtcatca atggtatagc ttatccttgc tgcagtgtac
     8401 aaactgtctt tgcgccccgt taagaatacc cattgttgcg tattcaccct catcgaatca
     8461 gcatattttt tcatctgtgg aacagaatct gtctccgggt cgcaggtatg tgagaggatc
     8521 aggaaatctt tctcactctt caatttttca tataccagct tcaaattatt attcatcttc
     8581 ggacaaatgc ctttacaggt ggtaaaaaag tattctgcca catatacctt accggccaca
     8641 tctttttccg taaagggttt accatcctgt gtggtaaatg caaatggtct tacaaaactt
     8701 accggagcca ctcctatttt agtaaatccc ggaataaccc gtgccagaac aaaatagaaa
     8761 cctactgcca ggacaataaa aaaacaagta tacaaccaac cctttttcga cataatattg
     8821 aattaaagaa caaagttacg tcccggatag ttctgattaa cgaaggcata ttttcatttg
     8881 caactatgtt gcttttttaa tacctttgtt cccttttatg aaatttcata tcaactggga
     8941 tgcattaggg atcggggctt cactggcctg cgccattcat tgcgctttgt tgcccctgtt
     9001 tctcagcagt ctccctttgt ttggaatcaa catcatccat cacctgggtt ttgagatcgg
     9061 tatgatcttg ctggccctgg ctattggttc atattcaatg taccacgggt ttaagaaaca
     9121 ccatcatagc cttctgccgt tgtccctttt ctatgcagga tttattttcc tggttttaaa
     9181 acaattcttc ctgcattaca gtacctggct gctgatacct gccgtgatcc ttattgtaac
     9241 cggtcacctg ttaaattaca aatcatgccg ggtacataat catgcacatg cagacgattg
     9301 caaccattga ttctgttagg gagatatctc cgtctttttt tggtttatct tcatatgaat
     9361 aaacgattcg tatgaaatat ttccttgtgc tactcagctt ttgcagtgtc atgggatcac
     9421 aggcccaatc aaaaaatgaa acaacgatca ggaatattct gaacgaacaa accggggcct
     9481 ggaataaggg agatatcaat ggctttatga aagggtactg ggaaaatgat tcattgatgt
     9541 ttatcggcaa aactggtata acatacggct ggaccaacac cctgaacaat tacaagaaag
     9601 gataccctga tacagctgcc atgggaaaac tcacctttac cctcttctcc gttaaacggc
     9661 tttcaaaaag gcattatcat gtagttggca aatggttttt aaaaagaagt atcggcgatg
     9721 tgggcgggca ctatactctc ctgtttcaaa agataaatgg ccgctgggtt attattgccg
     9781 atcatagcag ttagcttgca gccataatta ttatactttc accactttta aatatacttt
     9841 gacatggcat ttattattct tggaatcctt gcacttatag ccggtatcgt tttgcaaaaa
     9901 aatgaacagg tagccccgtt tgcaaaagtt gccagattag ttgccgtact tttaataatt
     9961 gtgggtgtac ttaccaagtc tgttgttcag atcaatgcag gtcagatagg agtaaaaaca
    10021 ctgtttggca aagtacaaaa tgatgtatta cacagtggtc ttcattttat caatcccttg
    10081 atggaagtat taagcctgga tataaaaacg cagaattata cgatgagtgg tgtgcaggat
    10141 gaaggaaaca gatctgccga tgacgccatc agggtactga cggcagacgg actggaagtc
    10201 accattgacc ttactgtttt atataagctc cttccttctg aagcacccaa actggtaaga
    10261 gaaaccggtg aagactataa agataaaatt gtacgacccc tgacccggac aaaaatccgt
    10321 gataattccg tttattatga cgccatttcc ctttattcca gcaaaagaga tgaatttcag
    10381 cagcgcatct ttaaaagtat tgaagatgat tttaaaaaaa gaggtttgat actcgagcag
    10441 ttgctggtca gaaatatcac gttgccactt tcagtaaagg caaccatcga acaaaagata
    10501 aatgctgaac aggatgcaca gaaaatgcag tttgtcctgt taaaagaaaa gcaggaagct
    10561 gaacgaaaaa gagtggaagc acagggtata gccgattacc agcgcattat caatattggc
    10621 cttaccgacc agcagcttca gtatgagcag ataaaagcct acctggaact ggctaaatcg
    10681 cccaattcaa aagtaattat catgggaaaa gggaatgccc ctgtaatcct ggatacaaaa
    10741 aactaaacag gagcttcctc atatcgctgt tgcaatcttc cgaaaacgaa ataagaagtg
    10801 ataagtccaa acccggaaaa aaggatatca taagggtcaa acgtacgtcc gaatacaggg
    10861 atcagttgca ggtactcgtt aacaacaagc attccaaatc ccaggagaca aacaaagcgg
    10921 aggtcataac tgcactcaat gcggaacatt cttgccagca aaaaatcttt tccgctaaaa
    10981 aaccagcagg ccccgaatgg aattaaaaaa gaaccgatta agttgggtgc aattcccaaa
    11041 aagaaatgca tgggttcatc aaaatgatat aaaggacgga tcaggatttt tatccccaga
    11101 atgattatgg ctcctgtaat aacagaccat ttacttattt gctttaattc catgtactgt
    11161 gaaaatataa ttttctaaac taaccgactt tatttttcca gccatccgtg aaagagggta
    11221 ttttactaat aactacccgt ttttccatgt tttatacaaa taccgaacca gtaaaaaaag
    11281 agtaacagcc attactgcat aaaatataat ggggtagata acggattgac cattgaaata
    11341 tccaagttga aaataaagac ctgccgtggc acaagtcatc atccataaaa gggtaatggc
    11401 aattgaattt agaattttga caaataaatt tttgacctgt ggatcccagc cttgctccat
    11461 acatcagttt gaacggacaa ggtactacaa ttcgaaagcc gctgttggct ttgtttaccg
    11521 gatcagtatt cttttaaacg cagctgaatc cccattaaaa acattaaaat ccaccgggga
    11581 tataatgccg tttacccggc ccctgtcgct atgttgccag aatacccagc cccgcctgat
    11641 acggggctgc tctggctggt aataatgtgc cacccataat ggatattcat caaactggct
    11701 accaaggtga cgattataaa aatcaacatt tgtgtaaata aagggcttaa ccccatagtg
    11761 gctttcaatc agatccagcc attccctggc ttccttttgt agttgcaggg tacctgtacc
    11821 attcagctgt tccacatcca gtactggcgg aaaatcccct gcttccagtt caactttttt
    11881 aataaaattt tcagcttgcg ctttcccgtc ccgggatgca atgaaaaaat gataggcccc
    11941 cctgatgact ccggcttgtt tggccttcct ccagtttcta cggaactgcg ggtcagtatt
    12001 gcttatgcct tccgttgctt ttatgaaggc aaatccgagt tgaatttctt tcactttcat
    12061 tttcttcact ccttcccagg caatggtatg ctggtacttg gaaacatcaa tgccatgtat
    12121 ggagtagccc ccgggaatgg caatgccgaa ttccgggtaa cgggtatatt ttatttttcg
    12181 aaaagtcaac cactggtaca gcatccaggc cgctgttccc acgaaaacaa ctgcgagtac
    12241 ccgaaacact gaccggcgaa tcttcttttt ctgtttggca gatggcatac ggaatgtgat
    12301 gaatagtggt aaacgatcaa acaaatataa atatcaacct tgttgtaagt tgttaattga
    12361 ttgtattacc tgcctttcat tttttgtagg aggaacctta taacaattta ttgtaactta
    12421 tgcaggaaaa attaaaccga tgcctcgcct gcttattatt ggtggcaatg gtgtgctggg
    12481 aagcgctgct gccaatcatt ttcttgaaaa tggatttcgg gtaactgttt ttgtaagaaa
    12541 caggtccagg gctgtttcct tagagaaaaa aggagcaatt atagtagaag gagatctggc
    12601 aaaaccttcc acacttaccg gtattttcac aggcattgat attgtactta cggcggctca
    12661 tggcatgttg gggatgggca acaacaaatc tgaaaaggta gatggtaccg gtcataaaag
    12721 tttaattgaa gaagcccaaa aatccggcgt aaagcatttt atttatactt ctgtctttac
    12781 cgcctctgct gatcacccgg ttgatttttt cagaaccaaa tataaaatgg agcggttgat
    12841 aacaaaaagt gggctaaact atacgatcct gcaattacct gcctttatgg aatggcatgt
    12901 ttacaatttg cttggaaaaa atattgtcaa aaaaggtaaa acaaccatcc tgggccgtgg
    12961 caatacaccg gtaaacttta tagccgtaaa agatgtggta gccgctatag acaaaataat
    13021 actgaaggaa gagttttaca ataaaactat cccggtggcc ggtccgcaaa acatgacccg
    13081 taatgagatc gccgatttat ttggaaaggc gctgaatata aaacctaagg taggacatgc
    13141 gccggtacgt ttactaaaac tgttttccgt actagtcagc ccctttcacc cgggtattgc
    13201 aagagtaatg aaactgagta tttatacaga aaattcggat gaaaccatgg atccgaaata
    13261 tagtgtacaa caatttggat taagcccaac aacaattgaa gagtttattc agtcagtaac
    13321 aacaaaaaga tgatgcctgc catcattaca ctatccgcag ctttgcatag ttcagcataa
    13381 ttttcgtctc ccatatattc aagtattacg aaagtaattt tgtatgaaca gcatcttagc
    13441 atttaaatat attaccctgc tcctcaatca attcattttc cgggaattgg taaaaatgac
    13501 tatattaagg atataatact gcaaaaaaac actaaaccgt atgtcctttg taattgaaca
    13561 gttttatctt ttcttccagg gcgcattagt gttccaatct gttttctttt tccttatctg
    13621 ggccgccacc aggcgaaatg atgttctatg gtattcatta tacttactaa ccgcagccct
    13681 ttattttttc attaatgcca ccggaacgtt ttttaaaatt gaggaggccg ctgtttttga
    13741 atccagctgg tatagcttgc ttaatattcc aattattata attgaaaacc tattttacct
    13801 gctttttatc aaggcttttt tttatgatat tattgcatcc caaaaggtta aaaatatact
    13861 gcgtctgaca ttatattctg tgcctgtttt atttttttgt tttattattt tccgggtgac
    13921 gggtatcagc acctacaact tgttttatat tgtaaaactg ctaagcgtca ttcctgcgat
    13981 tttcattgca tatatagtta taaaaaacaa attgccctat gcaacgctgg tcgctaacgg
    14041 acttattttc actatcaccg gaaccctggt gactgtcata atgattattc tccggaataa
    14101 tgatgtgcac actatgttta ctgattacta tccgttgctt tttatacgac ttggaatact
    14161 aggcgatatg ttcttttacc agtttgcact gctaaaaaaa tggaggtggc aggaaaaaga
    14221 attagccctg cagcaacatc aaaccaagac agaagtagaa agagtacgta atgaaatcag
    14281 ccgggagttg cacgatgata tcggaagcac cctcagcggc attaatatgt atagtcatat
    14341 ggcaatgacg cagtcacaat ccggagacag taaagctacc gtacaatcat tgcaaacgat
    14401 tcagcaatcg gcagcggaaa tggttggaaa gcttaaagac atcgtctggg ctaataacct
    14461 gaaagatggg gatacagatg ctttttttga aacactaaag gactatgtat tgatgataac
    14521 aaaaccggca ggcattgttc cggaatttaa tttttcggca acagcagaaa caaaccggct
    14581 ttcaaaagaa gagaaatatc atatttatat gatttgtaaa gaggccgtga ataatatggt
    14641 aaagcatagt ggtgctgata gtcttgcggt ttcttttgaa aaaaaagata ctgggcttga
    14701 tattactata gccgataacg gatacggttt gaaaccgggg aaaaacaatg cgggcaatgg
    14761 ccttaataac atgcggaaga gggcagaaga aattggcgcc atcttcagct tgcagtcagg
    14821 agcaacggaa ggcagcaaaa tcagtttgta cttaaaaatc acccgttagg gtattataaa
    14881 acccaataag aaaaaacaac tttgcaaaat ggcagtaagg gtgacaatat ttgaagacag
    14941 taagcttata cgggaagcct tagaggctat aataaacggc actccgggat acacttgttg
    15001 tggtgctttc agcagtggca ataactggga acaagatata aaccgcagcc agccagatgt
    15061 gatactgatg gatatagaaa tgcccggcat ggatggtata caactcaccc gctgcatcac
    15121 ccaaaagttt gcgaatatta aagtgcttat acaaaccgtg tttgacgaga ataataaaat
    15181 ttttaatgcc ctttgtgccg gtgccagtgg ttatatatta aaaacagatc cgccgcataa
    15241 atacctggaa gcaatttccg aagtgaacaa tggagggtct tttatgaatg cagccgtagc
    15301 aaggaaaacg ctgaattttt ttgcacagaa aaatattatc ctggtgacgc cagagggtac
    15361 cgattaccag ctaagcaaaa gggaaaaaga agtactgatg gagcttatag atggaaaccc
    15421 attaaagaac atcgcagcca ggctgttcat tagcctggaa acagttcgta ctcatgtgaa
    15481 gcatatctat gaaaaactac atgtgacaaa ccggaccgaa gcaataatga aggcaacaca
    15541 acagggatta cagaaataaa taaaaaacgc cattatgaac tatgcagttt taataacagc
    15601 aatactgatt ttatcatgca gcaatccatc cgtaaaacag gaaagaaaaa cgaatgatgc
    15661 tgttcttgat gaacaaaaag agcaggctgc gattattaaa gtaatagaaa aggaaacgga
    15721 atgttttttt caacgggact ataattgctg gcaaagttgc tttgcacaaa cagactatgc
    15781 cttccaggca tggaacaatg aaaacggcac ttttgatgct tattccgggt gggaagcagt
    15841 aagtaatcat gcagctatct atttaaaaaa taaccctgtt gctaaaaata aagacatggg
    15901 ccaggaatac tcggcaaaag aaataaggag ttcacatcct aaagtaatcc ggaagaatat
    15961 gaaaataaaa ttcttcagcg ataaattggc ttacctcgta tgggatcaat acaatagtga
    16021 cacattttca caacgattta cctggagtaa agacagccgc ttaatggaaa aaattggcgg
    16081 agaatggaaa atcgtaaacg taacttctta ctgggatttt aagaatccca ttccggctga
    16141 tagccttcaa taaaaatcac ccatcgggtg attgcctgcc ccaccgctta cagatagctt
    16201 agcatcaaaa tttttttatg tgtaagcttc tccttcttat caccactaca ttttgcattt
    16261 tgcaaagcca tgcacaaaat gtgagtattg gcaccaataa taccagcaat gcaaagctga
    16321 cggtggaagg tgccgttggc cccgttgttg gtctgtttaa gaacggatcc ggaatttcgt
    16381 tgagtgtgtc cagtaataat gaaaatgtag gtttaaatta tgccgatgga aaagcaattg
    16441 ccaccggaat aggcggccgg ttattgctaa ataatagtgc ttctacgggc gggattggtt
    16501 tgcagtatta ttcttccacc acggctggtg ctacttttcc aacagctata aatgtactac
    16561 ggcataccga taacaggtgg gctggtagtg gtcattggat tgatatgctt tatggccagc
    16621 ctggcagggt taggactacc aatcagctat acactgaagc aaccggggag ttcaacctgg
    16681 tcccaatcgg cattatatat tttaaagtta ccaatatgca tcttggcagt aatagcactg
    16741 tggtaattaa aaatgcagga aacggtgcca ccatgagtaa ttcaatacta cagtttgtct
    16801 gcgacccggg aagtccaata gatgtcggca ttgtgagttt catcctgaat cttgactgga
    16861 ttacaaaaga atatgatgat gtttatgttg tgggtgttcc cagttttgca agctttactg
    16921 cagtaacata taccagtgcg gcagactttg taagaagacc ggcaaatcag caggattggc
    16981 tggaagtaac atacatggcc tccaggcttt acagcgattc acaattttcc ggaacactta
    17041 tgctatatgg caaccgcaca ttaaattaat aatataaaat cttatatatg aaaataataa
    17101 ccgcattggt attatatata tcctgcgccc tgttcatttc ttcatgttct gatagcaaaa
    17161 caaaagctca gcctgaagaa cagaaagaaa aagctactga aaagcaaacg atatctataa
    17221 taacagaaaa gcatgtgaaa attccgaata gtagtttata cattattcca cctgcaggct
    17281 ttgctgcaga cgaaacatct ggaaccttgg cacaaacaga aggaactgcc cattatatgc
    17341 agatgcaaat atttagtggg gaaacacagg agagcttatt tgcaaaattt aaatcggagg
    17401 cggataaaaa ttttcccgga agctggaaag aagaacctat aactattaac ggccatgcag
    17461 caactatttg ccactgtaaa tcagccggct actcacaata cttcatggct tttacagatg
    17521 gttatactga tgaaatgatt attgccaact atgaagaaaa agatgccgct actgctaaag
    17581 ctatgtacga agctttgaaa acagtggtgg taaaaaaata gatatttatt gtatgaaaat
    17641 tcttttttac acttttctgc ttgcaggaat tatcctggcc tgtaaaaaca gcaaggcgga
    17701 aaaacaatcc gatggaaata ataaaacgga aagtaaagca gatactgcaa gaatggccag
    17761 ttttcccggc actgctattt acatacaact acccaaaggc tttgtatgga atgaaacagc
    17821 gatgggtttt tataaagagg aagatggtag tgtaataaaa tataatgaat ttaaaaaaat
    17881 gcggtatgca gcgaatatgt cggcggaaga aacgatgggc tcactgatta accagcagcc
    17941 aatcaccatc aatggctata aaggattgat aaaaacttat cagcaaggta gtaccggcat
    18001 tcaactggag ctttcttttg gtgatgctag ctttatggaa tttgttgaag ccacttattt
    18061 ctctcatcag gaacaaactg ccaaagatat tctggcggca ttgaaaacga ttcaggttaa
    18121 aaaataatag aggcattaaa tttttagtcc ccggtgaaag caatacaatt tttcatatcc
    18181 gctatattgt tttgtaccgc tgcctttacg caatcccctt cttgtagcaa tgtgattaaa
    18241 gaagatagtg tacgtttttc caggaaatct gttttcctga attgggctgc taagaagaaa
    18301 ttataccata tagccatgga actgaacagg aatcctgatt gcagaatagt ggttacaggg
    18361 tatggaaaca gttgtttaat ctgccagcaa acttcctggg acaggtctta ctctgtagta
    18421 caatatttgc gacaaggcgg agtagattct ttacggtttg ttttcaggta cgcccaagaa
    18481 gcgtatagcc cttcggtggt atccatacga aactctctac caggagaaga tggccctgca
    18541 ttggatgaac ctccttcgcc ctgttatagc tatcacggct ttcataaaaa aagatgccgg
    18601 ggcaagcggc tctaactttt tatctcaatt aaaaatcacc caacgggtga ttgtttgctg
    18661 ctgttgtaat gaataggttt aatgtgggaa tgccaaaata atacccacaa taattaaaat
    18721 catattccaa taaatcttac accatgctca gacatttttt gctatttatt tcagcatcat
    18781 tcatcaccgc aacttcactg gcacaaacct atggaatagt aaagtttaac aatgttcctt
    18841 ttacgaatgg tagtgaagca ggtaaaacca gctttaaatc caatgagttt atttatgccc
    18901 gggtagaact ggaaaaatcg gtgaaagaat attttaaaat aacaaacccc gatggttcgt
    18961 caaagcctca tgtattaatt tttaagtttt caaagagata cacagaggtg gatgggaaaa
    19021 actactcaga taattttact aatggcgaat acttatatat aaaaccagag gatctttcta
    19081 aaacttatat agacctggat atactacccg atccggccat ggcgaatgat gtattttgtt
    19141 tggttagcaa ttttcagggc ggaatgtaca ccagcccaat ttacagagta aggccgaaag
    19201 gatataaaaa taattctacc ataaactata gcatccagtt agtgggtggc actaccgtgg
    19261 caaacaattc tggcaatgaa aatttgccaa aagttaccgg cacatttgat ttacttgtta
    19321 accattccga cgaaccttta ctaaaacaaa atagcgatct ggccgataaa aaagtgcaag
    19381 ccaatggttt gaacctttca agtcttccta ccatattctc caggccattt aaaactactg
    19441 acccaaaact aacggccgca aaactaactg ctattcttaa acgagactat ccgcaccgta
    19501 aggttttaaa aatggcatta gactccgatg gaggtcagct ttggttggtt tccaaaaatg
    19561 acttcggtat accacgctac cggtatttta atggtttgtt gcatgtggca tatatggagg
    19621 atggggtatg caaggtgggt agcgtagaac ttatagaaaa ctatctggga ggtggcaagt
    19681 atgctgcatt agcggctgac tactggtctg agtatgatag gacgatcaac tgtacggcgg
    19741 tcaagtaaaa atcacccgtt gggtgattga ttaaatgtca caccaggggt atgtttaatt
    19801 attaatcata aaactttaaa aatgaaattt caactatttc tctgcgttac gctaatagca
    19861 agccagttat tgtatggcca gttacccaca ggccccgatg gaaaaacact ttatcaaaaa
    19921 ggtgactgta aaacaatact gcctaatgta aaatacaaat gtgtcttttg tgaagacaaa
    19981 gaacttacaa aaaactgtaa ggagtatgat tgttctctga cagaatgtaa agaaagtaaa
    20041 agcacaaagg gaagcaatgg aaggatgcag gctaagccga taagtattga tggtaaacaa
    20101 gtaagaatgc gagatgatag cgatacaaca agcaaattgc ccaaaggcac aaaatttgaa
    20161 aatggaaaag tagtaattac aaacggatac aaagccgttc aatcatctga taaaacaatg
    20221 gtttttattc ttgctgataa tggaaataat gttgagggtt cgttttcttg taaatgtttc
    20281 ttgggccttg gtgcatgtgg tgtgataatt tctggctcag cagttggctg ctctggtgct
    20341 gagtgctgta ggttcaaggt aactacaggt aatttatccg ggcttacaat gcaagatata
    20401 gaaagagccc ccgaaaaatt aaaatggaaa aatttggtat tgcctgtaaa atcaaattaa
    20461 aattatttta atgaaaaaat atcttatttg cattctattg attgtatttt ataatcgtat
    20521 tgatgcacaa acggttaccg taattgacca tcgcggtgga aaagacactg tgaaaccaac
    20581 tactgtaata gatcatcgca ttgtcaggga tatgtcgcca ggtttagtaa aaccggtatt
    20641 tgaagaaatg cctgtctatc gtatccagtt acgtatcaca acatctgata ataatggaac
    20701 cgacgatgct gtatgggtgg agctgaatga agaagggcat aggttttacc ttgctaaagg
    20761 gattgataat tttcaaccac gaaaaacggt aatatatgat gttgtggatg cagacattaa
    20821 gaagttaaaa gatatccggt ttttaaaatt tggcgtaaaa ggcgaagacg gaccctgttt
    20881 caaaaaaatt gaacttattg ttaacaattc gggcacgcca atttatacta cacaaattgt
    20941 ttcaaatgcc ggaacttgtt ttgataacaa caatagtcag gtggccgaaa attttattgt
    21001 tacctataac aatttgcgta atcaccctta ttggaactat aacggcgcaa gggccaatat
    21061 ctggcggcct gtcaaaaaaa tcaataaaga gtgggtagta agtttaattg aggcttgtat
    21121 cggcaaccag atggtgcagg aaggatcaaa attaaaatgg gggagccatg gcggtgtttt
    21181 tgaaaacaat accttatttg gcgatgccgt ggaagtgaaa tacaagaaca atcacacact
    21241 tagtgttgat ttggacttag aaagatcaat cacaggatct aaccccgagg ccaacataaa
    21301 atttgaatta gacttcaggt gtaatgatgg ggtaattaat attgaaactc aaaatgtaac
    21361 agtgagtgcc gatggcgttg gtgctgtgca ggattttatc agaacaactg gttttgacct
    21421 ggtaagcatt gctattggtt tatttgcagc acccgctgcg gcaacaccgc ttacagttgg
    21481 tgcctggttt gcactaagaa gagccattgc atttagtata aaattaaatc ctgccactcc
    21541 caatacttca aatagttgca cacaaactat agtaaccgaa gtcggtgata tactagtaaa
    21601 aaaataaaat cacctaaaat ttattattat gaaaattaaa tctttgctta aagcgattac
    21661 tgtattcctt ttcattcttc ctttttgtac ttctatttct gcgcaagatg cacaaattga
    21721 tggaaaatcc tacgagacag taaggaaagt aaatctaaat gcagatgccg agtttgtaaa
    21781 gttgaaacaa aaaagcgctg gcacagcaag cggggaatca tatattaaag tgaacaatga
    21841 gtacgctaaa acaagtttta cagcagcagc attaaagaat ttttttgcaa caggtaagct
    21901 ttcatcactc ggaacggtaa agaaaacaat attggccggg aacccggtta tgtatataga
    21961 caacggctca tcaattgcga ttgttaatta taataaggag tcaattggct ttattgaaat
    22021 acagaatcca aaacaaatcc cgacagtaag aaatcattta gcaaaagctg gtaataatga
    22081 cgatcccgga ggttctactg gcgatccttt gctggactgt ggcgttgtat gcctcaacac
    22141 attcatcgct tgcggaaatt ctgctgtatg ccgcagtgct tataaaaaat gctggagcga
    22201 ttgtaagaac aggcatcaat cacagggagg atctggagga accccttctt attatattgt
    22261 atcattaaat gcactggcag taaaatttta aatatgcagg tgaatactaa aatagctgca
    22321 ttgtttctat attgtctggc cttctttatt tcttgtaaga gtactgacaa aaaagataat
    22381 gaagaaggta aaatttctgc tttcgttaag tcaactgccc ataccaacat ccccggcacg
    22441 catattttta tgataccacc ttccggcttt aaagcaagca ccggcagcat gaaaggcttt
    22501 atcaaagatg aaaccaccac tttcttatcg gtaacggaat ttaaaggcaa aagctttgtc
    22561 gcagaagcag cgaacaactc tgctgaaaaa atgaaggcaa aaggatttag catttttatg
    22621 gaagaagaaa cgaccgttgg cccttacaaa gcaagaagaa tgcggattgg cgatggagaa
    22681 acttccactg tctggcaact tctatttggc gatgatagtt ttgccgtaat gatcatgggt
    22741 atttatcctc cccatgatga aatcagtggt aagcaaatag ccgaagccat tgaaacagta
    22801 gtatatgata aggatatggt ggtagatgaa atggcaggta tgaattatac tatcaagtca
    22861 aatgaaagca ggtataaatt tttaaaaata caggccggac ttgcatttta ttcactagag
    22921 ggtaatgcag atttaaaaaa tgacgatata ccattcctgg tactgtcaca agtgccgcgg
    22981 gaaaatgcaa gtgcagaatc gtttgccgaa acagctttgg aatcaacact acgcaatgga
    23041 ttttcgaatc cctcaataaa aagcaaaggc tttttaacca ttaacaataa cagtgcttat
    23101 gaaatggaag tagcatgcat gctgaaaggc aaaccggtaa tcctttatat tgccgtggtg
    23161 acaaaagacc gtactgcagt aatggtacaa ggggtggcca agaaaaatat tgccgaaacc
    23221 attacaggct tcaaagattt tgcggatgca attgtaataa aataagttta ctccactatg
    23281 cgcagcttcg catagttgag cataattttc ttttcgccat tgtgctcaaa ctttaccgta
    23341 gcaatgggat tatgcgccgc tccttccatt ttcagcactt cgccaaaacc aaatttctgg
    23401 tgctccactt tttgcccggc ttgtaagtta gatgtatcgc tggcttcaaa gtcagcagaa
    23461 ggtacatgct cttttacttt gggcggcact cctttgggtg gcaaatatga tggtgcatat
    23521 tttgatgcgg cagggctgga attatagtag gtgcctgatt gctgcctggc tgaaccggac
    23581 tcaccatcat caacattcct ggctttcatc cggtcaaatg cagagccttt actccacttg
    23641 cttccactca cctggttttt agcccctccg cctgcaaaac ttctgtccat ggactgctca
    23701 ggaatttctt caatgaaacg gctgggttca ttttgcacta actggccaaa ccggtaccgg
    23761 gtattggcat aagtgatcca gaggcgttgt ttagctcttg taaccacgac atagaataac
    23821 cgccgttctt cttccagttc ctctcttgta ttaatagcca ttgcattcgg gaaaagcatt
    23881 tcttccaggc ccgctgcaaa cacacaactg aactcaaggc ctttggcagc gtgtatggtc
    23941 atcagcttta ccgtatctgc atccgggtct ttctcatcgg catccgtcaa caaagtgatc
    24001 tgttgaagat aagaagcaag gcttttatct accaactcac catcttcatt atcggggctt
    24061 tctgtccatt ccttaatgga gttaagcaac tcttgtatgt tctcgtatcg ttgtacacct
    24121 tccgtactct tatcgttgaa gagttcttta accaggtttg tttgctttcc tacatgaaat
    24181 gctacttcat acgcattttg ttttgtcagc atactggcaa aactctttat catgatcaca
    24241 aattcttcca gtgcagtaaa ggttccagcc ctgaaaccaa attcattggc tttttcgagc
    24301 acctgccaca tacttacatt attctcattg gccgacagaa ccaatttatc tacggttgtt
    24361 tttccaattc ccctggccgg gtagttgata attcttttca gcgcttcttc atcacttggg
    24421 ttgacgatga tgcggagata agccacaaaa tctttgatct cttttcgctg gtagaaactg
    24481 ataccaccgt aaatagtata cgcaa
//
LOCUS       DAORTI010000046        23575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1737_length_23575_cov_6.712372, whole genome shotgun sequence.
ACCESSION   DAORTI010000046 DAORTI010000000
VERSION     DAORTI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 23575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23575
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_23575_cov_6.712372"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLO70_07945"
     CDS             complement(<1..359)
                     /locus_tag="PLO70_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQZ74434.1"
                     /translation="MQSFEQLSEKFTLHFDHPHFPAEPASLYEPNEYFLRLGGKRVRP
                     VLCLMGNELFEEIKTDAWDVATAIELFHNFTLIHDDIMDKAPLRRGMETVHTKFGDNT
                     ALLAGDVMLVKAYEYLS"
     gene            complement(375..2393)
                     /gene="rnr"
                     /locus_tag="PLO70_07950"
     CDS             complement(375..2393)
                     /gene="rnr"
                     /locus_tag="PLO70_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQZ74435.1"
                     /translation="MGRKNSKKKKDKQRNKQSSANVLKGKLDITRSGMGFVTVEGMDV
                     DIMIRPADFNTAMHGDTVRVVIKEMRSSGKRMQGVVKDVLQRKRTEFIGELKMNKGFA
                     FFIPESDKPMPDFFIPLSNINKAQDGDRVVVKLQEWENDGNKRPVGEVLTVLDAENTN
                     DAAMKEIMLDAGFPLQFSDEALEVAARIPDVISREEIKRRKDIRDVFTITIDPADAKD
                     FDDAISFRKLKNGNFEIGVHIADVSHYVEPDNDLDKDAYQRATSVYLPDRVNPMLPEH
                     ISNVLCSLRPKEDKLTFSAVFQISPKAEVKQYWLGKTVIHSDHRFTYEEVQAIIEEKA
                     GLHAEEVLILNDLAQRFRKKRFNNGAINFSSQEVKFKLDDKGDPVGIMIKESKEAHQL
                     IEEFMLLANRTVAENISKLKVNNKPLPFPYRTHDDPDEEKLVPFAAFAKKFGHTFDTS
                     SPDTIAESFNQLLKDVHGKPEQHVLEQLGIRTMAKAKYTTENVGHYGLGFEHYCHFTS
                     PIRRYPDVLVHRILFDVLNNKIQPDKKLEEKCKHCSDRERSAMEAERAANKYKQVQYM
                     KNYLGEEFEGVISGVASFGFWVETVEHKCEGLVSVNSLLEYDEFRHVETDYCLVGQRS
                     GKKFRMGDKVWIKVVAANLTKRQLDYEWVVAGELATEKITKEKGKKRK"
     gene            complement(2403..3476)
                     /gene="lpxK"
                     /locus_tag="PLO70_07955"
     CDS             complement(2403..3476)
                     /gene="lpxK"
                     /locus_tag="PLO70_07955"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQZ74436.1"
                     /translation="MFSNFFLKSFRILLLPFALLYWLGIAIRNWLYDKRILESTSFGL
                     PLICVGNLSVGGTGKSPMVEYLVANLKNKFKVATLSRGYKRKTRGYAMADEKTTALEI
                     GDEPMQFHLKFPEVPVAVGEERILAIPQLLHDRPETKVIILDDAFQHRTVKAGLNILL
                     TDYNNLFTRDFYLPTGDLRDLKSSYKRAEIIVVTKCKKDLGEVEKQKLIKEINPLPGQ
                     LVFFTAINYSRPYHIISKMEKGLDKKVEILLVTGIANPRPLKKLLEEHSNTYQMLQYA
                     DHHIFTIDDLKEIKQAFNKIDAGEKVILTTEKDAVRLLKFSDEIKDLPFYVVPVRHQF
                     LFQEESKFDQLVSDFVLNFKKED"
     gene            complement(3466..4035)
                     /locus_tag="PLO70_07960"
     CDS             complement(3466..4035)
                     /locus_tag="PLO70_07960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQZ74437.1"
                     /translation="MTKKELRKIFRDKRNSLSMSERAKMDDLMLIQFQALKLPVIYSL
                     LSYWPIERNNEPNMHLFTDYMAFRNPELIIAYPKTDLVANNMHAISTNESSRFAINDF
                     GIYEPEKGEEIAAGYFDMIFVPLLAIDKKGYRVGYGKGYYDKFMVDCREDCVRIGFSY
                     FEPIQEMPDKHEFDVPLNLCITPQSVYVF"
     gene            complement(4042..4587)
                     /locus_tag="PLO70_07965"
     CDS             complement(4042..4587)
                     /locus_tag="PLO70_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQZ74438.1"
                     /translation="MRIIAGEHGGRKFNPPTNMPYTRPTTDIAKEGLFNVLQHNLDFD
                     ELKTLDLFGGTGSISYELASRGVPDLTIVEKDATMYEFIKKTSATLRLENFKVVKMDV
                     FKFIQSCTDKFDFIFAGPPYALTSIDDLPKHIFEQQLLKKGGWFVLEHTPRNDYKAFP
                     FYKSERNYGTTVFSTFVENRD"
     gene            complement(4575..5387)
                     /locus_tag="PLO70_07970"
     CDS             complement(4575..5387)
                     /locus_tag="PLO70_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HQZ74439.1"
                     /translation="MKQIFNIAKGKIENVQQVLLLKFGTNHGCFAICNKSGDELYRLA
                     YCKTEEWDSNNLSEFATAFPELNNTFYSVQVSYDFPESTLAPLKDYRQEEYGSLLKAL
                     YGINGTSAIVTETIPEWQLYNVYAVPVELRQWISQHFPIASYRHQCSLTARQINRENS
                     NGTLLVDFRTDDFTVTAARQDILLLSQYYPYSTPGDVVYYLLKICHQFSLPQQEVQLQ
                     LSGLIDRQSALYKELYQYFIKIDFRDASWNIPDSEYPNHFFTSVNDLAQCVS"
     gene            5456..6817
                     /locus_tag="PLO70_07975"
     CDS             5456..6817
                     /locus_tag="PLO70_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQZ74440.1"
                     /translation="MAGAVHNNLQVAISNRQILKIAMPISLAIFIPQLNFITNNIFLG
                     HYSTDNYALAVAGITGVYYLIFAAIGYGLNNGLQALIARRAGENRPEEIGKLFTQGVL
                     IGLLVAGLGILITWTITPYLFGLIIHDPEVHKKAVEFLRIRIWGLPFLYIYQLRNALL
                     VGTNQSRYLVAGTLAETVTNIGLDYVLIFGMLGFPELGFNGAAIASVIAEFVGMFVIF
                     LVIRWKGISQRFGLFQDLKWNKENSSLIFSMSAPLMFQHAISIISWQFFFLLTEHHGD
                     MALKVSNVMRNIFGVFGCVTWAFAATTNAMVSNIIGQGKKEQVNSLLKKIIKLSTGVA
                     VVVCLLLNLFPEIFLSIFGQGDDFVAAALPVLRVVSAALILMSFSIITLSAVTGSGNT
                     RVTFFIELFAIIMYCIYVYLVSEYYFLPIKFGWMSEWLYWLCLFVPSLLYIRSGQWKK
                     KVI"
     gene            complement(6814..7164)
                     /gene="folB"
                     /locus_tag="PLO70_07980"
     CDS             complement(6814..7164)
                     /gene="folB"
                     /locus_tag="PLO70_07980"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQZ74441.1"
                     /translation="MTIELKELRFYAFHGLYGEEKKTGNEFEVSLCVDFSPVETVITN
                     IAVTVNYARLYEIVKTEMQKPRALLETLAMEIVESIHVSFPQINKADISIKKLHPPII
                     QFTGSVGVRYQKEF"
     gene            complement(7185..8126)
                     /gene="trxB"
                     /locus_tag="PLO70_07985"
     CDS             complement(7185..8126)
                     /gene="trxB"
                     /locus_tag="PLO70_07985"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQZ74442.1"
                     /translation="MEASEKVQCLIIGSGPAGYTAAIYASRAGLNPVLYQGIQPGGQL
                     TITTEVENYPGYPDGIQGPEMMVHFEKQAARMGADIRYGLATKVDFTGPVHKVQIDEE
                     KWIEADTVIISTGASAKWLGLESEQRLNGFGVSACAVCDGFFFKGKEVAIVGAGDTAA
                     EEALYLSKICSRVHMIVRRDEMRASKVMQERVKNTANIKIYWNSETDEVLGDKKVHAV
                     RIRNKKTGETEEVPVSAFFVAIGHQPNSDIFKGWLDMDEAGYLKTIPGTSKTNVEGVF
                     ASGDVQDKVYRQAVTAAGSGCMAALDAERYLSAKGVH"
     gene            complement(8258..9142)
                     /locus_tag="PLO70_07990"
     CDS             complement(8258..9142)
                     /locus_tag="PLO70_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HQZ74443.1"
                     /translation="MKIQLIAFVAISLSFVSCEKTVTFNLDEVSPKLVVEGTIENEQP
                     PVIYLSRSLNYFSQISPDILANSFARNAEVFVSNGTLTHKLKEYAVPIGGGYNVYYYS
                     IDSSDLTTAFTGQLNTNYSLRIIWEGKTYTATTRIPGITRQIDSFFWKPAPAGNPPEK
                     VSVMLKATDPAGLGDYVRYFTKQNSDPFYPGLNSVYDDQVIDGSTYEVQVERGILRTG
                     STPEAYSFFERGDTVTLKLSNIDKATYDFWRTMEFTYASVGNPFSSPTKVMSNIKGGG
                     LGYFGGYASQYRTIIIPQ"
     gene            complement(9250..10125)
                     /locus_tag="PLO70_07995"
     CDS             complement(9250..10125)
                     /locus_tag="PLO70_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQZ74444.1"
                     /translation="MRQLKIATQITNRDSQAVEKYLQEISKISMITPEEETTLAQRIK
                     MFTRNPVDSQRALDKLVQANLRFVVSVAKQYQHQGLSLSDLINEGNLGLIKAAQRFDE
                     TKGFKFISYAVWWIRQSILQALAEQGRLVRLPQNKIGTYNKANKAYMAFEQEHEREPS
                     TEELAELLEMSETEINNIFQSNTRHTSLDAPVHEAEDVAMGDLLEGSDDTDDDVMKDS
                     LRNEIRRVLKSLSPREAEIVNAYFGLDGENGVTIEQIGQKYDLTKERIRQIKERAIKR
                     LQKARYSSALKAYLG"
     gene            complement(10396..11577)
                     /locus_tag="PLO70_08000"
     CDS             complement(10396..11577)
                     /locus_tag="PLO70_08000"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQZ74445.1"
                     /translation="MYPNLYYAFKDLFGVEWTFLRFVNSFGFFVAISFIISAIVLAAE
                     LRRKSKEGIFQPTEMQVMVGQPATFVDLALNFLLGFILGYKIVALFILDSSATEDPQA
                     FIFSSLGSWPAGIGLGLFFAAVKWWEKNKQKLDKPEKRTVRIWPQDRVGEITIIALVF
                     GLLGAKLFDIFENWGDFLVHPSDYIFSAKGLTFYGGLICAALAIWWYARKHKIGFWHL
                     NDALSPTMMLAYSLGRIGCQVAGDGDWGIENTAAKPFSWLPDWMWSYKYPHNVNEVGV
                     PIPGCTDTKFCNELPVGVFPTPFYEIIVCFILFLVLWSLRKRLKIPGTLMALYLMLNG
                     LERFFIEKIRVNTRLDFLGFQPTQAEVISTLLFLTGLVLWLWLNNRAKAQKSTNRTNK
                     A"
     gene            complement(11708..12124)
                     /locus_tag="PLO70_08005"
     CDS             complement(11708..12124)
                     /locus_tag="PLO70_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQZ74446.1"
                     /translation="MYPEEIVIPMKEELTDNGFSELMTSAEVESQLAKEGTTLVMINS
                     VCGCSAGTARPGVLMAVANTTKKPDFLTTSFAGFDIEAVKTLRQHLMPFPPSSPAIAL
                     FKNGQLVHFIERHQIEGRPAQVIAQNLIGAFEQHCN"
     gene            12354..12800
                     /locus_tag="PLO70_08010"
     CDS             12354..12800
                     /locus_tag="PLO70_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQZ74447.1"
                     /translation="MAYVKFNSRPFEGTFNNLVDDLFTELPVLFKNDFNNTERKGTIP
                     VNVKETEKSYQLEVVAPGFEKSDFKVNMEQDLLTISAEKKSEVKDENEKYIRREYSFR
                     SFKRSFTLDEKIDATNIEASYVNGVLTLNLPKKVAVKAAATEIIIK"
     gene            complement(12900..13358)
                     /locus_tag="PLO70_08015"
     CDS             complement(12900..13358)
                     /locus_tag="PLO70_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74448.1"
                     /translation="MKYFFILLTVITCGICAKAQTAEDSVKAVINNMFTGMKNADAVL
                     LKSSFADSMVLQTISRDKEGNLVVKNEDPAGFIDFVSKQKPGAADERISFETIKIDGP
                     LALVWTPYNFYFNGQFSHCGVNSFQVIRFSNGWKIQYIIDTRRRQGCSGQ"
     gene            13447..13788
                     /locus_tag="PLO70_08020"
     CDS             13447..13788
                     /locus_tag="PLO70_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74449.1"
                     /translation="MSKIVQAVILMTIVALAASCATGKEYAARIFSSRDLRIKDTTIV
                     AIRFLELDNDSLQDIKDGWVKNSFNTNIDSTKESNKVPVVVESKTPVLPNEPVAKTNK
                     PGEVRTKRTRE"
     gene            13855..14220
                     /locus_tag="PLO70_08025"
     CDS             13855..14220
                     /locus_tag="PLO70_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74450.1"
                     /translation="MKRTFILSIAAVMTAAVFTSCGHSRVWATKERRESAPPTPPART
                     ASYYNAAPLIVSPTPGFTMNRHPNGQYFHKSPQGIMYWKGYDNRFYLDRNYVNRTQYS
                     KWEYNEWKRYSRQSNSRYR"
     gene            complement(14262..15662)
                     /locus_tag="PLO70_08030"
     CDS             complement(14262..15662)
                     /locus_tag="PLO70_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQZ74451.1"
                     /translation="MTNSYHDLVKQTFNFPQEGIEVKDGNLYFNGLDLKALIAKYGTP
                     MKLTYLPKIGMQIKKAKTMFANAFKKHKYEGKYFYCYCTKSSHFSFVVEEALKSDIHL
                     ETSYAYDIEIIKKLYAKKKITKEINIICNGFKQKGYTRRIANLLNTGFSNVIPVLDNK
                     DELKEYAQSVKVPFKLGIRVAAEEEPNFPFYTSRLGIRAKDILEFYVDRIEGYETKFQ
                     LKMLHIFLNKGIKDDIYYWSELNKVINLYCQLKKICPELDSINIGGGFPIKHSLGFEY
                     DYQFMINEIVRNIKVACKKAKVPMPNIYTEFGSYTVGESMGHIFSVIAEKVQNDRETW
                     YMIDSSFITTLPDTWGIGEKFLMLPVNKWDQDYQRVVLGGITCDSHDYYDSEEHINEV
                     FLPKTNGTTEPLYVGFFHTGAYQDQISGYGGIKHCLIPSPKHVIVGYDKNGKLVDWVY
                     AKQQTAQSMLKILGYK"
     gene            complement(15758..16303)
                     /locus_tag="PLO70_08035"
     CDS             complement(15758..16303)
                     /locus_tag="PLO70_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74452.1"
                     /translation="MKWRHLSIQSKRNNMDYTDQTIMLTGYIISNLVALLMLWFSRRK
                     PVTARALFFLLFVWAGVTNATTSISTPGVYLEYADFTFLSFYKSIILGFFGRNITVIV
                     MCIAICQLLIGVSMILKGVIFKLGCIGGILFLIGITPLGVGSGAPATLTWAVGLYLLY
                     RRGAKNYWWSSFRKQQPSGII"
     gene            complement(16263..16739)
                     /locus_tag="PLO70_08040"
     CDS             complement(16263..16739)
                     /locus_tag="PLO70_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HQZ74453.1"
                     /translation="MSYLVVYMSHHGTTQKIACHIAEELGTGVTLVNLGENDVPDLTS
                     FSTIIVGGSIHMGLLQKKIIDFCEQNRETLLGKRLGLFLCFMNKAEGQKEFDKVYPSA
                     LREHALAKGLFGGELLLEKMNFVERMVVRTVKAVKKSVSEIEEDEVAAFIDTIKEK"
     gene            complement(16750..17565)
                     /locus_tag="PLO70_08045"
     CDS             complement(16750..17565)
                     /locus_tag="PLO70_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74454.1"
                     /translation="MIQETKNAIILWLSLPLAAIVIFVSVTGLATPGFYAAETPNWQA
                     QSYGQDLADLFLVTPALIISSIYAYQGRKGALFIWAGVIAYLIYTYLIYCFAIHFNKL
                     FVFYCLALGLSFYSLLWFFYSLLKRKGISEGFSSIPHRIIGIYFIIISCLFYFLWLSE
                     IIPAIMDGDLPESIKETGLITNAVHVIDLSVFLPGIFIAGVLILQRKILGYCLAPVFL
                     SFFILMNITIGGLVLIMKFKDLEGSYAVAAIMAVLALVSAVLLVWFIKRMQKS"
     gene            complement(17700..17897)
                     /locus_tag="PLO70_08050"
     CDS             complement(17700..17897)
                     /locus_tag="PLO70_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74455.1"
                     /translation="MKSSVMPSTVQLNEEAKIELTQEVEETVATEAINNKNETFTVSQ
                     MWNRQRQVRSASDRIRKWNLN"
     gene            complement(18184..20151)
                     /locus_tag="PLO70_08055"
     CDS             complement(18184..20151)
                     /locus_tag="PLO70_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74456.1"
                     /translation="MYFNKVSLVRVVRIVILLFAAFLYFIDAGAQLCQGSLGVPIINK
                     TFGAGTNPGNPLAAASTTYQYVSTDCPNDGYYTVRNNTSACFADSWHSIAADHTGDGS
                     GYFMLVNASMQPGAFYVDTVRGLCSGTTFEFATWIMNVLKTTACNPSPIMPNLTFSIE
                     RTDGTLIQNYSTGDIPSQANPTWQQFGFFFNTPVGISDIVLRIVNNAPGGCGNDLALD
                     DITFRPCGPQLTPAITGFPVNTDTLCEGSAASYTFTCAVSPGFSNPSFQWQQSFNGGP
                     FTDITGATNTTLVQNFLTTAAAGTYTFRLSAAEAGNMSSALCRVVSPLITIQVEKNPV
                     TTVTGISPVCERGVLSMAATGGSQYQWNGPAAFSSTSDVVTISNAQVSQSGKYYVLVS
                     NAAGCNHLDSITVMVNPSPTASTSFSTADLCEGDTLQLLGSGGTIYTWQPSAGLSSSV
                     LSNPLAFPLSSVLYSVIVGNSFNCTDTATIDLVVKTRPVANAGPDKITVAGLPVQLAG
                     SAGGQDITYTWSPALYLDDPLSLSPIASPLAEIDYMLSVTSNVGCGTATDVMKVYIYN
                     DVFVPTGFTPNNDGLNDTWNIPALAAFPNFELLVYNRNGEVVFQNSKTNKPWDGRYKG
                     LPQPSGVYIYVIDLKDSSRPGLLKGFVHIIR"
     gene            complement(20205..20600)
                     /locus_tag="PLO70_08060"
     CDS             complement(20205..20600)
                     /locus_tag="PLO70_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74457.1"
                     /translation="MEIEIFTLADFAQDNNSKLTIVGTFDSINSKQFPATHPSCSVAC
                     RLRFGIKEVGSHDFKLRLIDADGREMLKPVEGNINIGNPPNGQFASINIVVNFAQLQF
                     GKPGRYSFELYVDGEWKSGLPLFLNQVAG"
     gene            complement(20814..21707)
                     /locus_tag="PLO70_08065"
     CDS             complement(20814..21707)
                     /locus_tag="PLO70_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74458.1"
                     /translation="MKRRNFIYSSSLIAMSMGVFGKIKWDGHAYVGEDPTTTDILGPF
                     YRPGAPFRTELVQQGTKGQLLHFSGTVFAKDGTTPLKNSLVEIWHCDENGVYDNTSDD
                     YIYRASFKTGSDGNYHFKTILPVPYLAGPAMTRPAHIHMRVSGNTQQDLVTQVYFKGD
                     EYIAKDESAGDPRSLNRILESSTNSKNEKVVKFDIVLKDEYVLDAAAFKKLSGLYEMK
                     DKSMCEFYRQDDQLFVKVNGQIMEAMDYKGNNSFEGGLGRVKATFEFPGNGAVKVKAS
                     YMTEPDKYVTIEGDKMLKYPG"
     gene            complement(21870..22358)
                     /locus_tag="PLO70_08070"
     CDS             complement(21870..22358)
                     /locus_tag="PLO70_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQZ74459.1"
                     /translation="MKKIFLFFLAAGSLVFLSCNIEGGMSAAAKKNKETNDAIMKAYE
                     AGDFSKMGDYIAADAVDHGGETGDIKGLENIVNEMKRYHAMMPDMKATTSRSLADDEY
                     VFTWATMTGTMNGKAVTMSSVDVSKFKDGKAVEHWVYMDPKEMMQMMPPAPAPVTDTL
                     KK"
     gene            22530..23297
                     /gene="hxpB"
                     /locus_tag="PLO70_08075"
     CDS             22530..23297
                     /gene="hxpB"
                     /locus_tag="PLO70_08075"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HQZ74460.1"
                     /translation="MKNSSTKLILSEKLPLPRGEIALKEFGKLHRLMNVCSMQYKAVI
                     FDMDGLLIDSEPLWREAGRETIQQFGKDLTEEQYHSSTGLRTEEWIEHWFHYFNIDMT
                     HAPTAIDIIIRKAIEKIERHGKAFPGIDTIFQFLKQRNFKLGLATSSPLSLVEVVIRK
                     LNLENSFDTIASAGELPYGKPHPQVYIDCSIQLNCSPLQCIAVEDSFNGMIAAKAARM
                     KCVVVPATAEYEHPKWNAADLKLKSLADFTEEKLNLI"
     gene            23365..>23575
                     /locus_tag="PLO70_08080"
     CDS             23365..>23575
                     /locus_tag="PLO70_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74461.1"
                     /translation="MKFHTAILLTTFLLVSPILYSQQPPPPPPADTIEVNDKIFDRVE
                     FEADYPGGINEWVKYLQRNLRADVPV"
BASE COUNT         7048 a         5156 c         4362 g         7009 t
ORIGIN      
        1 ttgctgagat attcataagc ttttaccagc atcacatcac cggcaagcaa ggccgtatta
       61 tcaccaaatt ttgtatgaac agtttccatt cccctccgta aaggcgcctt gtccattata
      121 tcatcgtgga tcagggtaaa attgtgaaat aattcaatag ccgttgccac atcccaggca
      181 tcggtcttta tttcttcaaa tagttcgtta cccatcaggc aaagtaccgg acgaactctt
      241 tttcccccaa gtcttaaaaa gtattcgttg ggctcatata aagaagccgg ttctgccgga
      301 aaatgcggat gatcaaaatg aagtgtgaat ttctcagata attgctcaaa actttgcata
      361 ggccaaaata agtatcattt ccgtttttta cccttttctt tcgtaatttt ttcagtagcc
      421 aattcacctg ccactaccca ctcataatcc agctgtcttt ttgtgagatt tgcagccacc
      481 acttttatcc aaaccttatc tcccattctg aatttcttac cacttcgctg acccactaaa
      541 caataatctg tttcaacatg cctgaactcg tcatattcca ataaactgtt cacactgaca
      601 agcccttcac atttatgttc cactgtctct acccaaaagc cgaatgaagc aaccccacta
      661 attacacctt caaattcttc tccaaggtaa tttttcatat actgcacctg cttgtactta
      721 ttagcagccc tttctgcttc cattgcggat ctttccctgt cactgcaatg tttgcatttt
      781 tcctcgagtt ttttatctgg ttgaatttta ttgttcagta catcaaaaag tatgcggtga
      841 accagtacat caggatagcg ccgtatcgga gatgtgaaat gacagtaatg ttcgaaacca
      901 aggccataat ggcctacatt ctctgtagtg tattttgcct ttgccatagt ccggattcca
      961 agttgttcca gtacatgctg ttcaggtttt ccatgcacat ctttcagcag ctgattaaat
     1021 gattcagcta tagtatcagg cgaagatgta tcaaatgtat gaccgaactt tttagcaaaa
     1081 gctgcaaaag gaaccagttt ttcctcatcg ggatcatcat gtgtacggta aggaaaaggc
     1141 aaaggcttat tattgacttt cagttttgag atattctcag ctactgtccg gtttgccagt
     1201 aacataaatt cctcgattaa ctggtgggct tctttacttt ctttgatcat gatcccaacc
     1261 gggtcgcctt tatcatccag tttaaatttt acttcctgag acgagaaatt aatcgccccg
     1321 ttgttaaatc ttttttttcg aaaacgctgg gcaaggtcat tcaggatcaa cacttcttcc
     1381 gcatgcaaac ctgccttttc ttcaataatt gcctgcactt cttcataggt aaagcgatgg
     1441 tcggaatgaa tcactgtttt ccccagccag tattgtttca cttcagcttt tggagatatc
     1501 tgaaatacag cagagaaggt gagcttatct tcttttggcc gcaatgaaca aagaacattt
     1561 gaaatatgtt caggtagcat cggattcact ctgtcaggca ggtaaaccga agtggcccgc
     1621 tgatatgcat ccttatccag atcattatcg ggttcaacat agtggctgac atcagcaatg
     1681 tgtacaccga tttcaaaatt accattcttc aatttccgga atgatatagc atcatcgaag
     1741 tcctttgcat ctgcagggtc aatcgtaata gtaaaaacat ccctgatatc ttttctgcgt
     1801 ttaatttctt cccgggaaat cacatcaggg atccttgcag ccacttctaa tgcctcatcg
     1861 gagaattgca gcgggaagcc tgcatccagc attatctcct tcatcgctgc atcattggtg
     1921 ttttctgcat ccaaaacggt tagcacttcc ccaaccggtc ttttgttgcc atcgttttcc
     1981 cattcctgta gttttacaac aaccctgtca ccgtcctgcg ctttattgat atttgaaagt
     2041 ggaataaaaa aatcaggcat tggcttatca gattcaggaa taaaaaaagc aaacccttta
     2101 ttcattttca gttccccgat gaattcagtc cgctttcttt gcaagacatc ttttaccacc
     2161 ccctgcattc ttttcccgct gctacgcatt tctttaatga caacccgaac tgtatcgcca
     2221 tgcatggccg tattgaaatc ggctggccgt atcataatat ccacatccat tccttccaca
     2281 gtgacaaaac ccatacctga acgggtaata tccaatttcc ctttcaggac atttgcagat
     2341 gactgtttat ttctctgttt atcttttttc ttttttgaat tttttcttcc cataataata
     2401 atttaatctt ccttcttaaa gttcagtaca aaatcagata ccaactggtc aaacttgctt
     2461 tcctcctgga aaagaaactg gtggcgaaca ggaaccacat aaaaaggcag gtctttaatt
     2521 tcatcactga atttcagcaa cctcactgca tctttttctg tagttaatat aactttttct
     2581 ccggcatcaa tcttattaaa tgcctgtttt atttctttga gatcgtcaat agtaaaaata
     2641 tgatgatctg catactgcaa catctggtaa gtattactat gttcctccag caacttcttc
     2701 aaaggtcttg gattggcaat acctgttacc agtaatatct ccaccttttt atccagcccc
     2761 ttttccattt tcgagataat atgatacggc ctgctgtagt taatggcagt aaaaaatacc
     2821 agttggccgg gtaatggatt tatctcttta attagtttct gtttttcaac ttcccccagg
     2881 tctttcttac attttgtaac cactattatt tcagcccttt tgtaactgct tttcaaatcc
     2941 cttagatctc cggtgggcag gtaaaaatcc cttgtgaaaa gattattata atctgtaagt
     3001 aatatgttca gccccgcttt aacagtacga tgttgaaatg catcgtccag tataataact
     3061 tttgtttccg gcctgtcgtg caataactgg gggatggcca gaattctttc ttcaccaact
     3121 gccaccggca cttcaggaaa tttcagatga aactgcatag gttcatcacc gatctcaagt
     3181 gctgttgtct tttcatccgc cattgcatag cctcttgttt tccttttata cccccggctg
     3241 agtgtcgcta ctttgaattt gtttttaaga tttgcaacca ggtattccac catcggcgat
     3301 ttacccgttc ctcctaccga tagattccct acacatatca atggcaatcc aaaggatgtt
     3361 gattccagta tccttttatc gtataaccag ttccggatag ctattccaag ccagtacaac
     3421 aatgcaaaag gcagtaagag tatacgaaat gacttgagaa aaaaattaga aaacataaac
     3481 agattgaggc gtaatacaaa gatttaaagg tacatcaaat tcatgcttat ccggcatttc
     3541 ctggatcggt tcaaaatacg agaacccgat tctgacacag tcttccctgc aatcaaccat
     3601 aaacttgtca tagtaacctt ttccataacc aaccctgtac cctttcttat ctatcgcaag
     3661 aagcggaaca aatatcatat cgaagtaacc ggcagctatc tcctcacctt tctcaggctc
     3721 atagatgcca aagtcattta ttgcaaaccg ggatgattca ttggttgaaa tggcatgcat
     3781 attatttgca acaagatcag ttttgggata agcgatgatc aattccggat tcctgaatgc
     3841 catgtaatct gtaaacagat gcatattggg ttcattattt ctttcaatcg gccaataaga
     3901 aagtaaggaa taaataacag gaagtttgag agcttgaaat tgaataagca tcaggtcatc
     3961 catcttagcc ctttcggaca tggaaagtga gtttctttta tccctgaaaa tctttctgag
     4021 ttcttttttg gtcatgtagt attaatccct gttctcaaca aaagtagaga aaacagtagt
     4081 accatagttc cgttcagatt tatagaaagg aaatgctttg taatcgttcc tgggtgtatg
     4141 ctccagcaca aaccatccac cttttttcaa caactgctgc tcaaaaatat gttttggcaa
     4201 atcatcaata ctagtcaatg catagggtgg gccggcaaaa atgaaatcaa acttatcagt
     4261 acaactctga ataaacttaa atacatccat ctttaccact ttaaaattct ctaaccgcaa
     4321 cgttgcggag gtttttttta taaactcata catagttgca tccttttcaa caatcgtcag
     4381 atcaggaaca cccctggaag ccagttcata actgatgctg ccggtacccc cgaacagatc
     4441 cagggtcttt agttcatcaa aatccaggtt atgctgcaat acattgaaca gcccttcttt
     4501 ggcaatatcg gtcgttggcc ttgtataagg catattcgtt ggcgggttaa attttcgtcc
     4561 gccatgttca ccagctatga tacgcattgg gccagatcat taacagaagt aaaaaaatgg
     4621 tttggatact cactatcggg gatattccag gaagcgtctc tgaagtcaat tttaataaag
     4681 tactggtata actccttata caaagcggat tgcctgtcaa ttaaacctga taattggagt
     4741 tggacttcct gctgtggcaa ggagaattga tgacatattt tcagtaagta ataaactaca
     4801 tccccgggag tggaataagg gtaatattgt gacaacaata atatgtcttg ccttgcagca
     4861 gttactgtaa aatcatctgt tctgaaatca accaacagag ttccattact attttccctg
     4921 tttatttgcc gggcggtaag ggaacactga tgcctgtatg atgcaatggg gaaatgctgg
     4981 cttatccatt gccttaattc aaccggtaca gcatatacat tatacaattg ccattcagga
     5041 atcgtttcag taacaatagc tgatgtaccg tttataccat acaatgcttt taacaaactc
     5101 ccgtattctt cctgcctgta atcctttaat ggagccaatg tactctccgg aaaatcataa
     5161 gaaacctgaa cactataaaa tgtattgttc aattctggaa atgcagtggc aaactcagat
     5221 aagttattag aatcccattc ttcagtcttg caataggcaa gcctgtataa ttcatcaccg
     5281 gatttattac agatggcaaa acagccatgg tttgttccaa atttcaataa cagaacctgc
     5341 tgaacatttt caatctttcc ctttgcgata ttaaatattt gtttcaaaat attttctcaa
     5401 ttcctgcaat aatagacaaa aatttaaata tcgtaggttt gccaaccttt tactaatggc
     5461 aggtgcagta cataataact tacaggttgc aatttccaac cggcagattc ttaaaatagc
     5521 aatgccgatc agccttgcta ttttcattcc acagctgaat ttcattacta ataatatatt
     5581 tctcggccac tactccactg acaattatgc tttggctgta gcaggcatta caggtgtcta
     5641 ttacctgatt tttgctgcca ttggttatgg cttaaacaac ggacttcagg ccctcatagc
     5701 ccggagggcc ggcgaaaacc ggcctgaaga gattggtaaa ttatttacac agggagtcct
     5761 gataggtttg ctggttgcgg gtttgggtat acttatcacc tggacaataa cgccctattt
     5821 attcggcctg attatacatg accccgaagt acataaaaaa gcagttgaat tcctgcggat
     5881 aagaatttgg gggctgccat tcttatatat ttatcaacta cgtaatgcat tattggtcgg
     5941 caccaaccaa agccgctacc tggtagccgg gaccctggct gaaacagtta cgaatattgg
     6001 acttgattat gtattgattt ttggtatgct tggtttcccg gaactgggtt ttaacggggc
     6061 tgccattgca tcagtcattg ctgaatttgt tggcatgttt gtcattttcc ttgtcatccg
     6121 ctggaaaggg ataagccagc ggtttggctt atttcaggat ttaaaatgga acaaagaaaa
     6181 cagttctctt attttctcca tgtctgcccc gcttatgttt cagcatgcca tcagtatcat
     6241 cagctggcaa tttttctttt tgctcacaga acatcatggt gacatggcgt taaaagtttc
     6301 caatgtgatg cgaaatatat tcggtgtatt tggctgtgtt acctgggcct ttgcagctac
     6361 caccaatgct atggtaagta atattatagg acagggtaaa aaagaacagg taaacagcct
     6421 cttaaagaaa atcatcaaac ttagtacagg cgttgcggtt gtagtttgtt tactattaaa
     6481 tctcttccct gaaatttttc tatccatttt tggacaagga gatgattttg tggcagccgc
     6541 tttgccagtg ctgcgggtag tttcggcagc attgatatta atgtcatttt ccattattac
     6601 actgagtgct gtaacaggaa gtggcaatac aagagttaca ttttttattg aactctttgc
     6661 tattatcatg tattgtatct atgtgtacct tgtttcagag tattatttcc tgcctataaa
     6721 atttggctgg atgagtgaat ggctttactg gctctgcctg tttgtaccat cactattata
     6781 tatacgaagt ggtcaatgga aaaagaaagt gatttagaat tctttttgat accgcacccc
     6841 tacactacct gtaaactgaa taatcggcgg atgaagtttt tttatagaaa tatccgcttt
     6901 attaatttgt ggaaatgaaa catgaattga ttcgacaatt tccattgcca gcgtctccaa
     6961 taaagcacgg ggcttttgca tttccgtttt tacaatttca taaagacggg catagtttac
     7021 tgttacagca atatttgtaa tgacagtttc aaccggggaa aaatcaacac acaggcttac
     7081 ctcaaactcg ttaccggttt tcttttcttc cccgtaaagt ccgtgaaagg cataaaatcg
     7141 aagttcctta agttcaatcg tcattatacc cattatagaa taaattagtg aacgcctttt
     7201 gcacttaagt aacgttctgc atctaatgcg gccatacaac cgctaccggc agcagttacc
     7261 gcctggcgat atactttatc ctgtacatca cctgaggcaa acacaccttc cacattggtt
     7321 ttactggtgc cgggtattgt tttgagatac ccagcttcat ccatatccag ccatccttta
     7381 aaaatatcag aattaggctg gtgaccaatg gccacgaaaa atgcacttac aggcacttct
     7441 tctgtttcac ctgttttttt attcctgata cgaactgcat gtaccttttt atcacctaac
     7501 acttcatccg tttcgctatt ccagtaaatc ttaatattag ccgtattttt tactctttcc
     7561 tgcatcactt tgctggcccg catttcatcc cggcgaacga tcatgtgaac ccgtgaacaa
     7621 atctttgata agtataaagc ttcttctgcg gctgtatcac cagctccaac aatcgctact
     7681 tcttttcctt taaaaaagaa cccatcacaa accgcacagg cacttactcc aaaaccattc
     7741 aacctttgct cactttcaag acccagccat ttagcggagg ctcccgttga tattattaca
     7801 gtatctgctt caatccattt ttcctcatca atctgaactt tatgtacggg gccggtaaaa
     7861 tccacttttg ttgccaaacc ataacgaatg tctgcaccca ttctggcagc ttgcttttca
     7921 aaatgtacca tcatctctgg tccctggatg ccatctgggt aaccaggata gttttcaact
     7981 tccgttgtta tggtcaactg accacctggc tgaattccct ggtataaaac ggggttcaaa
     8041 ccagctctgc tggcataaat agctgcagta tatcctgcgg ggcctgaacc aatgatcaga
     8101 cactgtactt tttctgatgc ttccatgatg agatcttatt tataaattga atagtaacca
     8161 atccggttag caaatttagc agtaaaaacg caatagtctt tgtaacatac atcaccatat
     8221 tatatggctg tacaaaacta tattcatacc ggacagccta ttgagggata atgatagttc
     8281 tgtattgcga agcatagccg ccgaaataac ctaatccacc acctttaata ttactcataa
     8341 ctttggtagg agaagaaaaa ggattgccta cactggcata agtaaattcc atggttctcc
     8401 aaaaatcata agtggcttta tcaatattgc taagttttag agttactgta tcacctctct
     8461 caaaaaagct ataagcctcg ggagtagaac ctgttctcaa aatccccctt tcaacctgta
     8521 cttcataagt agagccatca attacctggt catcataaac tgagtttagc cccggataaa
     8581 aagggtcact gttttgtttg gtaaaatatc ggacgtaatc cccaagtccg gccggatccg
     8641 tagctttaag catcacagat actttttcgg gagggttgcc tgccggagct ggcttccaga
     8701 aaaatgaatc gatctgcctt gtaataccag gtatcctggt agtcgcagta tatgttttac
     8761 cttcccaaat gatccgcaat gaataattgg tattcaactg gcccgtaaat gcagtggtaa
     8821 gatcggaaga gtctatcgag taataataaa cattatagcc tccgccgatt ggcacagcat
     8881 actcttttaa tttatgtgta agtgttccat tagaaacaaa cacttccgca tttcgggcaa
     8941 aactatttgc taaaatgtca gggctaattt gtgaaaaata attcaggctg cgggaaaggt
     9001 aaataacagg tggctgttca ttttctatag tgccctccac cactaatttt ggagaaacct
     9061 catcaaggtt aaatgtcact gtcttttcgc aggatacaaa tgacagagaa attgcaacaa
     9121 aagctatcaa ctgaattttc atatcatatt aataactgcc gggaatagaa aatgttctgc
     9181 aaaaaaaatc cggcctcaca aaatgtgaaa gccggaacta atttaaatta acccattcat
     9241 ttgaatatat taccccaaat aggctttcaa tgcactgcta taccgggctt tctgtaaacg
     9301 tttaatagcc ctttctttga tctggcggat acgttctttg gttagatcgt atttctgccc
     9361 gatctgctca atagtaactc cattttcgcc atccaggcca aaataagcat ttacgatctc
     9421 agcttccctt ggtgatagtg atttcaacac ccggcggatc tcgtttctca atgaatcctt
     9481 catcacatcg tcatcagtat catcactgcc ttccagtaag tcacccattg ctacgtcttc
     9541 agcttcatgc acaggtgcat ccagtgaagt atgacgggta ttgctctgga aaatattatt
     9601 gatctcggtt tcgctcattt ccagcaattc agccagctct tcggtagaag gctctctttc
     9661 atgctcctgt tcaaaagcca tataggcttt atttgctttg ttgtaagtgc caattttatt
     9721 ttgtggcagg cggactaatc tgccctgctc tgccaacgcc tgtagtattg attggcgaat
     9781 ccaccacaca gcgtaagaaa tgaatttgaa acctttggtt tcatcgaagc gttgtgccgc
     9841 tttaattaag ccgaggttac cctcgttaat aaggtcactt agtgacaagc cctggtgttg
     9901 atattgctta gccacagata caacgaaacg aagattggcc tgaacaagtt tgtccaaagc
     9961 ccgttgagaa tctacagggt tcctggtgaa cattttgatt cgctgggcaa gagttgtctc
    10021 ttcttccggc gtaatcatcg agatcttgga gatttcctgt aaatactttt caaccgcctg
    10081 ggaatccctg ttggtgatct gggtagcaat tttgagctgc ctcataactt aagaaattta
    10141 tatatcaaca aatagaaaac agtctttgtt tgcaggttat tgcaaatgga tagttaatgc
    10201 agaatgtgaa tttaccgtcc gggactgtct gcaaaagtag ctaataatag ccgtttctca
    10261 tagcgtttag gcgtgaaaaa ttacccacaa tgatagcgca atcgattccg tatttaaact
    10321 actcgtggaa atgcagttct ggcaagggtt tgacaaaaaa agatttaaat taaaatctta
    10381 caaaaaaagt taaaatcagg ccttgtttgt tcgatttgtg gatttctgtg cttttgctct
    10441 attgtttaac cagagccata ataccagtcc tgtaaggaaa agcaaggtag aaatcacctc
    10501 cgcctgtgta ggttgaaatc ccaaaaaatc caatcttgta ttcacccgta ttttttcgat
    10561 aaaaaaccgt tccagtccat tgagcatgag ataaagagcc ataagggttc ccggtatttt
    10621 aagtcttttt ctaagtgacc aaagtacaag aaacaggata aagcatacta ttatctcata
    10681 aaaaggtgtt ggaaagaccc caaccgggag ttcattacaa aattttgtat cagtacagcc
    10741 tggaataggc accccgacct cattgacatt atgtggatat ttatacgacc acatccagtc
    10801 aggcaaccaa ctgaaaggct ttgcagcagt attttctata ccccagtctc catcaccggc
    10861 gacctggcaa cctattctac ccagagagta agccagcatc atggttgggg atagtgcatc
    10921 attcaggtgc caaaatccaa ttttatgctt tctagcatac caccagattg caagagctgc
    10981 acaaatcaat cccccgtaaa atgtgagtcc cttggccgaa aaaatataat cagaaggatg
    11041 aaccaaaaaa tcaccccagt tttcaaagat gtcaaatagt ttggctccca acagaccaaa
    11101 aacaagggca atgatagtta tctcccctac cctgtcctgt ggccaaatcc ttacagttct
    11161 tttttcgggt ttatcaagct tttgtttatt cttctcccac catttcacgg cagcaaagaa
    11221 aagtccaagc ccgattcctg caggccagct tcccagggaa gagaagataa aagcctgtgg
    11281 atcctcagta gcggaactat ccagaataaa caacgccact attttataac caagaataaa
    11341 acccagtaag aaattaagcg ccaggtccac aaaagttgcc ggttgcccaa ccatcacctg
    11401 catttcggta ggctgaaata ttccttcttt acttttacgc cgtaattccg ctgccagtac
    11461 tatagcagaa atgataaatg aaatggcgac aaaaaaacca aaagaattaa caaaccgaag
    11521 aaatgtccac tctacaccaa aaaggtcttt aaaagcgtaa tacagattgg gatacatgtt
    11581 attaattaag gaaagcgaat atactaaaga aagtttcgag ttatgagttt cagggtgcga
    11641 cttttactca aaacaaaaaa gctccatacc tgagggtacg aagctaatag ttatgccttc
    11701 tgatttttta attacaatgc tgttcaaatg caccaatcag gttttgggca attacctgtg
    11761 ccggcctgcc ctctatctgg tgtctttcaa taaaatgaac caattgcccg tttttgaaca
    11821 aagctatagc aggagaagat ggcgggaacg gcattaaatg ctgacgcaat gttttaacgg
    11881 cttcaatatc aaacccggca aatgaggttg ttaaaaagtc aggctttttt gttgtattag
    11941 caaccgccat taacaccccg ggtcttgcag ttccggcaga gcaaccacat acagaattga
    12001 tcattacaag tgtagtacct tcttttgcta actgagattc tacttccgcc gaagtcatta
    12061 attcagaaaa accgttatcg gtcagttcct ctttcatcgg gattactatt tcttcaggat
    12121 acatattatt tgttttaaat ggaacgcaaa aataaagaaa atgtttatag caggcagaag
    12181 tcattactgc agaaagaaat tatgtcagta aaaattcccc atttcggaaa ttatgtcttt
    12241 tccctattga actttttacc atgattgaca caaaaaatgt tcaattacca gggtggtaca
    12301 tggtttgacg attattctgt gttaaaacaa ttcatattta aaactaaata actatggctt
    12361 acgtaaaatt caacagcaga ccatttgaag gcacattcaa caatttggtt gatgaccttt
    12421 ttaccgaatt accagttttg ttcaaaaatg attttaacaa tactgaaaga aagggtacca
    12481 ttcctgtaaa cgtcaaagaa acagaaaaga gttaccaact tgaagtagta gctcccggct
    12541 ttgaaaaatc agattttaag gtaaatatgg agcaggatct gctgaccatt tctgccgaaa
    12601 agaaaagtga agtaaaagac gaaaatgaga agtatatccg cagggaatac agcttccgtt
    12661 cttttaagag atctttcaca ttagatgaaa aaatagatgc aacgaatatt gaagcatctt
    12721 acgtcaatgg tgtgctgaca ttaaaccttc ccaagaaagt agcagtcaaa gcagcagcga
    12781 cagaaataat aattaaataa gttcttacaa taaaaatttt ctcataagca gttggttttg
    12841 gtttacaccc tccgtttcca cggagggttt ctttttacca ctactatatt tttatcctat
    12901 tattgcccgg aacatccctg ccttcttctt gtatcaataa tgtactgaat tttccatccg
    12961 ttgctgaaac ggataacctg gaaagaatta accccgcaat gactaaactg accattaaaa
    13021 taaaagttgt atggcgtcca tactaaagcc agtggcccat cgattttaat tgtttcaaaa
    13081 ctgatccgtt catctgctgc tcccggcttt tgtttgctta caaaatcaat aaaacctgcg
    13141 ggatcctcat ttttcacaac gagatttccc tctttatctc ttgagatcgt ctgcagcacc
    13201 atactatctg caaaactgct tttcaataat accgcgtcag catttttcat accggtaaac
    13261 atattattga ttacagcttt tacagagtcc tctgctgtct gcgcttttgc acatatccca
    13321 caagtaatta ctgttaatag tatgaaaaag tatttcatct tctgtgattt taagatttta
    13381 tggcaaatga attgaacggt tattacgttg tttcctgtat ctaaaataaa gcctctagcc
    13441 atgcaaatgt ccaaaatagt acaagcggtt attttaatga caatagtggc attggctgca
    13501 agttgtgcaa cgggtaagga atatgctgca cggattttca gttcaaggga tctccgtatc
    13561 aaagacacca cgatagttgc tatacggttc ctggaactgg acaatgacag cctccaggat
    13621 ataaaagatg gctgggtaaa aaattctttc aacaccaata ttgattcgac aaaggagagt
    13681 aataaagtac ctgtagtagt ggagtcaaaa acacccgtcc tccccaatga accggttgcc
    13741 aaaacgaata agccgggtga agtaagaacc aaacgaacca gagaatgaat tcaaaaagcc
    13801 aatccctgtt gcggataaat atgttcacat ttcttaatct ttaaaaacag tctcatgaaa
    13861 agaactttta tcttatctat agcagcggta atgactgctg ctgtttttac cagttgcggg
    13921 cacagccgtg tgtgggcaac aaaagaaaga agggaatcag caccccctac acccccggca
    13981 cgcacagcat cctattacaa tgctgctcca ctcattgtaa gcccaacccc gggattcaca
    14041 atgaaccggc accccaacgg acagtatttc cataaaagtc cgcagggaat tatgtactgg
    14101 aaaggctatg acaatcgatt ttacctggac agaaattatg tcaaccgtac ccaatacagc
    14161 aaatgggaat acaatgaatg gaaacgctac agcaggcaaa gtaatagccg gtacaggtag
    14221 aaaaaacaat aaaaaccctt tcaattgaaa aggtttacat tttacttata tccgagtatc
    14281 ttcagcatac tctgtgctgt ttgctgtttg gcatataccc aatcaacaag ctttccgttt
    14341 ttgtcatacc cgacaatcac atgtttggga gaaggtataa ggcagtgttt gataccacca
    14401 taaccactaa tctggtcctg gtaagcgccg gtgtgaaaga atccgacata taagggctca
    14461 gtagttccat ttgttttggg gaggaacact tcattaatat gctcttctga atcataataa
    14521 tcgtggctgt cacaggtaat gccgcccaat accacccgct gataatcctg gtcccattta
    14581 tttaccggca acatcaggaa tttttcacca atgccccagg tatccggtaa ggttgtaata
    14641 aaggaagaat cgatcatata ccatgtctcc cggtcgttct gtactttctc agctattaca
    14701 ctgaaaatat gacccatact ctctccgacc gtgtagctgc caaattctgt ataaatattc
    14761 ggcattggca ctttggcctt tttacaagcc actttaatat tccgtacaat ttcattgatc
    14821 atgaattggt aatcatactc aaaacccagt gagtgtttga tcgggaatcc gccaccgata
    14881 ttgatagagt ccagctcggg acaaattttc tttaactggc aataaagatt gatgacctta
    14941 ttcagctcac tccagtaata gatatcgtcc ttaataccct tgttcaggaa tatatgcagc
    15001 atcttcaact gaaacttggt ttcatagcct tcaatcctgt ctacataaaa ctccaggata
    15061 tcttttgcac ggatacccaa tcttgaagta taaaagggga aattaggctc ttcctccgca
    15121 gccacccgga tgcccagctt gaatggaact tttacagatt gcgcatactc ttttaattca
    15181 tctttattat ccagcaccgg tatcacatta ctgaaacctg tattcagcag gtttgctatg
    15241 cgacgtgtat atcctttttg cttaaacccg ttgcagatga tattaatttc ttttgtgatc
    15301 ttctttttag cgtacagctt cttaatgatt tcaatatcgt atgcatatga tgtttccagg
    15361 tgtatatcac tctttagcgc ttcttccact acaaaactga aatgagaact cttggtgcaa
    15421 taacaataaa aatacttacc ctcatattta tgttttttaa aggcattggc aaacattgtt
    15481 tttgcttttt taatctgcat cccgatctta ggcaaataag tcagcttcat aggcgtaccg
    15541 tatttggcga tcagtgcttt caggtcaagg ccattgaaat aaagattgcc atcttttact
    15601 tcaatacctt cctgcggaaa attgaatgtt tgctttacga ggtcgtggta agagtttgtc
    15661 attgttgaat aacgggttag gccagtattt taaaaccgta caaaagtata ggtttggaga
    15721 aattgaaggg aaaaaatttg agaattgccg gtcaggctta aataatacct gacggctgct
    15781 gctttctgaa actactccac caatagtttt tagctcctct cctgtaaagt aaatacagcc
    15841 caacagccca ggttaatgtt gcgggggcac cggaacccac tcccagcggg gtaataccta
    15901 tcaaaaatag aataccacct atacaaccca gtttaaaaat cacccctttc agtatcatag
    15961 aaacaccaat caataactgg caaatggcaa tacacattac gattactgtt atgttcctac
    16021 caaagaatcc gagtattatg ctcttataaa aagataaaaa agtaaaatcg gcatattcaa
    16081 ggtatacacc aggtgtactt attgaagtag tcgcattggt aacccctgcc catacaaaca
    16141 ataaaaagaa taaagccctt gccgtcaccg gttttctccg gctaaaccag agcatcagca
    16201 acgctacgag atttgaaata atatagcccg taagcattat tgtctgatca gtataatcca
    16261 tattatttct ctttgattgt atcgataaat gccgccactt catcctcctc aatttcggaa
    16321 accgattttt tgacagcttt taccgttctt accaccatcc tctccacaaa attcattttc
    16381 tccaatagta gttcaccgcc aaataaacct ttggccagtg catgttctct caatgctgaa
    16441 ggataaactt tatcaaattc tttctgccct tctgctttat tcataaaaca aagaaacaat
    16501 ccaagtcttt taccaagcag tgtctcccta ttttgctcac aaaagtcgat gatctttttt
    16561 tgtaaaagtc ccatatgaat agagcctcct acaattattg tggaaaatga agtcaaatcc
    16621 ggcacatcat tttcaccaag atttactaac gttacacctg taccgagttc ttctgcaata
    16681 tgacaggcaa ttttttgagt ggtgccatga tggctcatgt aaacaactag ataactcata
    16741 atacaggttt taagattttt gcattctttt gataaaccat accagcaaaa cagcactgac
    16801 taatgccagc actgccatga tagcagcaac cgcataacta ccttccaggt ctttgaactt
    16861 catgatcagc accagtccgc caatagtgat attcattaaa ataaagaagg acagaaacac
    16921 cggggccaaa caatacccca atattttcct ctgaagaatt aagacccctg caatgaatat
    16981 tcccggaaga aaaacagaaa ggtcaataac atgaacagca ttggtgatta aacctgtctc
    17041 ctttatactt tcagggaggt caccgtccat aatggcagga ataatttctg acaaccagag
    17101 aaaataaaat aagcaggaaa taataataaa ataaatacca attatgcggt gtggtatact
    17161 actaaaacct tcggatattc ctttcctctt caagagagaa taaaagaacc atagcaagga
    17221 ataaaaagat aatcctaagg caagacaata gaaaacaaac agtttattaa aatgaattgc
    17281 aaaacaataa atgagataag tataaatcag gtaagctatt acaccagccc agataaacaa
    17341 tgctcctttt cttccctggt aagcatatat ggaactaata atcaaagcag gagtaactaa
    17401 aaacagatct gctaaatcct ggccgtaaga ttgtgcctgc cagttgggtg tttctgctgc
    17461 atagaacccc ggagttgcca atcccgtcac actaacaaaa atcacaattg cagccaatgg
    17521 caatgaaagc catagtataa ttgcattttt ggtttcctga atcatcaggc aaaactataa
    17581 atgcccggat gctggaagtt tgaccctgct cataaccaga actgaagtgc atcaagggcc
    17641 tgaaagtgac cgggaacagg tcaaaaacaa aaaaccgcct ttgaaaggcg gctttttgtt
    17701 tagttcagat tccatttacg tatcctgtca ctggcagaac gcacctgtcg ctggcggttc
    17761 cacatttgtg atacagtaaa tgtctcattt ttgttgttga tagcttctgt agctaccgtt
    17821 tcttctacct cttgtgtcaa ctcaattttt gcttcttcat tcaattgcac agtagagggc
    17881 ataacactgc tcttcataaa tctccttttt ttaatctgtc gtttaatttt tagtctgagc
    17941 gaaacggaag taagagaacc aggtggcaag cggataaggc tgggtcaggt tacgttcttt
    18001 gtagttttgg gtacgttgct cagaattgcg atgcaaaagt acgactgata aggcttctat
    18061 ccaaatcaca atttgaaaaa aatgttaaag aggaaaataa tatttatact taccaagtat
    18121 aattcagcat taaaaaaaat ttatgccact cattaaataa ttgtgtaact aaaacgaaat
    18181 attctacctg ataatatgaa caaacccttt cagtaaacct ggcctggagc tatcctttaa
    18241 atcaatcaca taaatataaa caccgctggg ttgcggcaat cctttatacc ggccatccca
    18301 tggtttattt gttttcgaat tttgaaaaac cacttcgcca ttcctgttat agaccaataa
    18361 ttcaaaattg ggaaaggctg ccagcgcagg aatattccag gtatcgttta aaccgtcatt
    18421 attgggtgta aacccggtgg gaacaaatac atcattgtag atataaactt tcattacatc
    18481 tgttgccgtg ccgcacccca cattagaggt tacactcaac atataatcta tctctgcaag
    18541 cggagacgca atgggtgata aggataacgg atcatccagg taaagagcag gcgaccaggt
    18601 gtaagtaata tcctgtccac cggcactgcc ggctaactgc accggtagcc ctgctacggt
    18661 tattttatca ggaccggcgt ttgctacagg cctcgttttt acgaccaggt caatggtggc
    18721 agtatctgtg caattaaatg aattacctac aataacacta tataatacag atgacagggg
    18781 gaatgccagc gggttggata aaacggaaga agataatcca gctgatggct gccaggtata
    18841 aatagtacct ccgctgccaa gcagttgcaa agtatcacct tcacaaagat cagcagtaga
    18901 aaatgacgtg gatgctgtgg gtgatggatt taccataacc gttattgaat ccaggtggtt
    18961 acagcctgcg gcatttgaaa caagtacata atattttcct gattgagata cctgtgcatt
    19021 gctgatcgtg acaacatcac tggtactcga aaaagctgca ggcccattcc attggtattg
    19081 tgaaccgcct gttgcggcca tagacaatac acctctttcg catactggtg aaatacctgt
    19141 tacagttgtt actggatttt tctcaacctg gatggttatc agtggtgaaa ctaccctgca
    19201 cagtgccgag ctcatattgc cagcttctgc tgctgataac cggaaagtat aggttcctgc
    19261 tgcggcagta gttaaaaaat tctgtaccag tgttgtatta gttgcaccgg taatatcagt
    19321 aaagggtccc ccgttaaaac tttgctgcca ttgaaaggag ggattactga atccgggcga
    19381 cactgcacag gtaaaagtat aagaagcagc cgatccttcg caaagtgtat ctgtattgac
    19441 agggaaacct gtaatagccg gcgttaactg aggcccgcag ggacgaaagg taatatcatc
    19501 cagtgcaagg tcatttccgc aaccacctgg ggcattgttt acaatcctta atacaatatc
    19561 tgaaatgcct accggtgtgt taaagaagaa accaaattgc tgccaggtag gatttgcctg
    19621 agaaggaata tcacctgtac tgtaattttg aatcaatgta ccatccgttc tttcaatact
    19681 aaaggtaagg ttgggcatga tgggacttgg gttgcaggcg gttgttttaa gtacattcat
    19741 tatccaggtg gcaaattcga aagtggtacc actgcataag ccccggacag tatctacata
    19801 aaacgcacct ggctgcatcg atgcgttgac cagcataaaa tagccactgc catcacccgt
    19861 atgatcagca gcgatcgaat gccagctatc agcaaaacaa gcactggtat tattccttac
    19921 agtataataa ccatcattgg gacaatcagt ggatacatat tgataagtgg tgctggcggc
    19981 agccagtgga ttgccgggat tagttccggc tccgaacgtt ttattaatga taggaacgcc
    20041 aagacttccc tggcataatt gggcaccggc atcaatgaaa tagagaaacg cagcgaataa
    20101 taatattaca atccttacaa ccctcaccaa actaactttg ttgaaataca aaattagaca
    20161 gattgcgtca gagttttatg attaaataat gatatgtagc tactttatcc ggctacctgg
    20221 ttcagaaaca acggcaagcc agatttccac tcaccatcta catataattc aaaagaataa
    20281 cggccgggtt tcccgaactg caactgcgca aaattgacaa caatattaat agacgcaaac
    20341 tgaccgttgg gcggattgcc aatattgata ttcccttcaa cgggcttaag catctctctg
    20401 ccgtcagcat ctattaacct gagtttaaaa tcatgactgc ccacttcttt tatcccaaaa
    20461 cgaagacgac aagccacaga acaggaagga tgagtagccg gaaactgttt cgaatttatg
    20521 ctatcaaaag tacctacgat ggttagcttt gaattattat cctgtgcaaa gtcagcgaga
    20581 gtaaaaatct caatttccat aatcgatatt aaatgtaaaa aatgtgtacc tgcaaagaca
    20641 gcaccaataa tatgtcatct gccaaataag caggcggtcc cggaaaataa atattgtgac
    20701 ctggggaacc ttaatactga tatttaaagc cggtggttag cctgtgactt accaccatta
    20761 ccatttatat tttccttcgg ttcaaaacac ttcaaagtat ggcttcaata aatttatccc
    20821 ggatatttta gcatcttatc cccttctatc gtaacatatt tatcaggctc tgtcatataa
    20881 cttgccttta ccttaaccgc tccattaccc gggaattcaa aagtcgcttt taccctgccc
    20941 agcccgcctt caaaactgtt gttaccttta taatccatgg cttccatgat ctggccattc
    21001 actttcacga aaagctggtc gtcctgccgg taaaattcac acatcgattt atctttcatt
    21061 tcataaagtc cggacaattt cttaaacgct gcggcatcca gtacatattc atctttcaac
    21121 acaatatcaa acttcaccac tttttcattt ttactgttgg tagatgattc aagtatacgg
    21181 ttgagtgacc tcgggtctcc ggcactttca tcttttgcaa tatattcatc ccctttaaaa
    21241 taaacctgtg ttaccaggtc ctgctgtgtg ttgccggaga cacgcatatg tatatgtgcc
    21301 ggcctcgtca ttgccggccc ggccaggtaa ggaacaggca gaatggtctt gaaatgatag
    21361 ttaccatcgc tgccggtttt gaatgaagcc cggtagatat aatcatccga agtgttatca
    21421 tacaccccat tctcatcgca atgccatatc tccaccaatg aatttttcag tggtgttgta
    21481 ccatcctttg caaaaacggt tccgctgaaa tgaagcagct gacctttagt accctgctgt
    21541 accagttctg tgcggaaggg tgcacccgga cgataaaatg gtcctaaaat atctgtggtg
    21601 gttggatctt cgccaacata ggcatgacca tcccacttga tcttgccaaa aactcccata
    21661 ctcatggcaa tcagtgatga actgtaaata aaatttcttc ttttcatgtt gaattgatta
    21721 ggtggtgaat aatactcagc gggtgagaat tggcagtgat cagagggatg aatttcgttc
    21781 gttggtaata gtaaggtaag taaattaggc gaatagcaaa aaaaggtccg tgctgccacg
    21841 aacctcttca ttaatcagct gaaaagaaat tatttcttta atgtatcagt taccggagca
    21901 ggtgcaggcg gcatcatctg catcatttct ttcgggtcca tataaaccca atgctccacg
    21961 gctttaccat ctttaaattt tgacacatcc acactggaca tagtaaccgc tttaccattc
    22021 atagttccgg tcattgttgc ccaggtaaaa acatattcat catcagcgag actcctggac
    22081 gttgttgctt tcatatccgg catcatagca tgatagcgct tcatttcatt gacaatattt
    22141 tccaatcctt ttatatcgcc tgtttccccg ccgtgatcaa cagcatcagc agcaatataa
    22201 tctcccattt tactgaagtc accagcttca tatgctttca tgatggcatc attggtttct
    22261 ttgttctttt tagcggccgc tgacataccg ccttcgatgt tgcaggacag gaatactaaa
    22321 ctcccggcag cgagaaaaaa taaaaagatc tttttcatgt tggtttgttt ttgtggaagt
    22381 aaaaggtaaa acaattttca gaacggccaa aaccacccat cctcaggtat tccgcctgcc
    22441 tacgaggcag caacgctatt cattgagaat taaggatcgg gaagtacaaa aaataattca
    22501 ggttacactg cttaatacaa agaagcagta tgaaaaactc atctacaaag ttaattttgt
    22561 ctgaaaaact acctttaccg agaggagaaa ttgcattaaa agaatttgga aagttgcacc
    22621 ggttaatgaa tgtttgcagc atgcagtata aagcagtcat ttttgatatg gacgggttgc
    22681 tgatcgacag cgaacctctt tggcgggagg caggcagaga aaccattcaa caatttggga
    22741 aagatctgac cgaggaacaa tatcactcca gtaccgggtt aagaacagaa gaatggatag
    22801 aacattggtt ccattacttc aatattgaca tgacacatgc cccaactgcc attgacatta
    22861 tcatccggaa agcgatagaa aagattgaac gccacggaaa agctttcccg ggcattgata
    22921 ctatttttca gtttttaaaa caacgaaatt ttaaattggg actggccacg tcttccccgc
    22981 tctcactggt agaagtggtt ataagaaaac tgaacctgga aaatagtttt gatacgattg
    23041 cttcggccgg tgaactgcct tacggtaaac ctcacccgca ggtgtatatt gattgttcta
    23101 tacaactgaa ttgctcaccc ctgcaatgta ttgctgttga agattccttt aacgggatga
    23161 tcgctgccaa agctgccaga atgaaatgtg ttgtggtacc tgccactgcc gaatatgaac
    23221 acccgaaatg gaatgcagcc gacctgaaac taaaaagcct ggctgatttt acagaagaga
    23281 aattaaattt gatataacta atacccgggc ggttatgcca ggccagttta aaaaaattaa
    23341 ataatttgcc caaaacagaa ctctatgaaa tttcacactg caatactact tactactttt
    23401 ctattagtat cccctatatt atattcacag caacctccac cgccaccacc agctgacact
    23461 attgaagtga atgataagat ctttgaccgg gtagagtttg aagccgatta ccccgggggc
    23521 atcaatgaat gggtaaaata cctgcaacgt aatctaaggg cggatgtacc ggttg
//
LOCUS       DAORTI010000047        21759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_1993_length_21759_cov_6.179598, whole genome shotgun sequence.
ACCESSION   DAORTI010000047 DAORTI010000000
VERSION     DAORTI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21759
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_21759_cov_6.179598"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..967)
                     /locus_tag="PLO70_08085"
     CDS             complement(179..967)
                     /locus_tag="PLO70_08085"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HQZ74462.1"
                     /translation="MVQAYNFLASWQLFPEKGTYEYGERPKSGIYKIEAVPDQKVLAI
                     YHNWVTLENQAFASAYKINADGDLNEFDNHELADKVQAAIPDGITFEIHFYKKGEIAL
                     HVVHEIMPNGYLRITQQGIKEDGSSYTNMEIYHKQLSVLPYSASVSGAVIKANEEGII
                     KHKALTAMEEQTNMQLDQIRKQIELLALQAHEIQKRKDLSLTIYSAKMSFKPNIGQTY
                     YLYEKQDETFILSLVSPKEWGKSGPFKRFVAAVQLLADHTWKEL"
     gene            1092..2159
                     /locus_tag="PLO70_08090"
     CDS             1092..2159
                     /locus_tag="PLO70_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQZ74463.1"
                     /translation="MKSYFGSYFISPGAEPVESSVLVFDKKMSIGFRNPDGSNGIVHW
                     PLTEVVVSYELSTQRTKVRHTGLGTELYMEGKEAAAFIKEMQDDLQRPWHKKNNSKDW
                     GRNLLLLSGFLGGLVLLYFLIVPWLAEKMASTVSVKTERQLGDAVYDAMGLSAAEDTA
                     ASFLLNEFFATMEVPSAYSVRITVVNDNVVNAFAVPGGRIVVYSALLKQIQSYPELAA
                     LLSHEFTHVNSKHSTKSIFRRLGSKVFIGLLFGSFGSVTSVLIDHADDLKSLTYSRKL
                     EKEADIKGLEILLQRKIDPKGFEDLFHHLKEASPASALPEFLGSHPDIEKRISYIKES
                     SKNATIVVDQRLQSIFEKIKQ"
     gene            2164..2481
                     /locus_tag="PLO70_08095"
     CDS             2164..2481
                     /locus_tag="PLO70_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HQZ74464.1"
                     /translation="MLITTTNVVDGRPVQEYLGVINAQSIIGANIFKDIFAGLRDVFG
                     GRSKTYEKVLEQAKEDALRELAEKAQALGANGILGVDLDFETIGSGGSMLMVIATGTA
                     VRL"
     gene            2516..3070
                     /locus_tag="PLO70_08100"
     CDS             2516..3070
                     /locus_tag="PLO70_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQZ74465.1"
                     /translation="MKRLLFSGFLCVLLSLQVMSQQTTVPAAAAIILKQAYREASEQN
                     KNVLVMFHASWCGWCHRMDTSINDPVCKKFFTDNYVICHLVVDESAEKKKLENPGAVE
                     LRTKYHGDGQGIPFWLIFDKEGRLLADSKIRTAGQGPEEGENVGCPASEKEVSYFISV
                     LKMTSPLNDQQLEVIRKRFRENEK"
     gene            complement(3076..5688)
                     /locus_tag="PLO70_08105"
     CDS             complement(3076..5688)
                     /locus_tag="PLO70_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ74466.1"
                     /translation="MKRFFIISMFFAGFSFSARSSHITGGEMFYTFTGMINGQYSYNV
                     TLKLFQRCGSGRQFPNPAIISIFNKTNNSRVTDISVTIGNQETISITNPDPCITNPPN
                     VCYEVAYYNFAITLPASAAGYILASHVNYRISGINNLSSGYNNVGATYTADIPGNISN
                     ITAPVNNSAFFTGNDLVVVCANNNFSYSFAAQDTDGDSLRYSFCEAYNSTAPGGGTAL
                     PPEPPPFPPVPYNYPDFYESQPLGGAVQINAATGLITGIAPDVGAYVVTVCVEEIRNG
                     IVIARQRKDIQINIADCDIAAASLQPEYLLCKNTQPITIANQSNSPLIVSTEWEFSDQ
                     TNTIIYTSADPIATYTFPAIGIYTVKLVINRNQTCSDSATALIRVFPGFTPGFTSSGI
                     CITNPTLFTDQTTSVYGTPNSWTWDFGETSSSNDFSSLQNPSYQYPQTGIKQARLIVT
                     DTRGCRDTITKPVTIIDKPPIQLAFRDTLICRNDMLLLQAGGTGNFSWSPLVNITNPN
                     TAMPSVSPTVTTKYYVELNESGCVNKDSVLIRVVNAVSLQAMNDTTICKGDTIQLKII
                     SDALQYVWTPGGQIINPAVKNPFVITNTVTSYQVTAIIGGCSAIENITVTPVPYPVVD
                     AGKDVSICNNASLLLNGITDGSSWSWRPANRLSNASILNPIAFPPRTTDYILTVTDTK
                     GCPKPVSDTIKVTVLPKMNVYAGADTAVIAGQPLQLNASGAAAYSWLPPDNLSASNIP
                     NPVAIFNDESASFRYKLIGYNEEGCKDSAYITIKVFKTGPTVFVPTGFTPNNDGKNDL
                     LLPIAVGMKSIEYFNVYNRWGQLLFSTQVNGQGWDGKINGQLQATGTFVWSVKATDYT
                     GKAYFQKGVATLIR"
     gene            5884..6375
                     /locus_tag="PLO70_08110"
     CDS             5884..6375
                     /locus_tag="PLO70_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74467.1"
                     /translation="MKLLLTIVLLLPLFSYAQNCKLNKETDPYTKETKLSTGFIIFSG
                     GSVTIDADSKEVDMLFSIEGANRCFDNNSTAVVFFEGSKAKMNLRNAGTMNCEGLFHF
                     IYKNSVNTTSQLQKLTTQKISQIVFTGNDKKEIKIILSPQNQEALVALGTCLVNEAKG
                     LIK"
     gene            6560..6991
                     /locus_tag="PLO70_08115"
     CDS             6560..6991
                     /locus_tag="PLO70_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74468.1"
                     /translation="MEKYQPGTPLVLHTGISLQQLVCNLIDRSRPVALHNRTHVVNQV
                     GRELVLGFDNENVVSVIRDLLSAIIENSCDGEIYIWAEKYSDVIMLLIEERNNNKGFA
                     LSFSIGSIEPYAARIGGHISIEGIRQRKTLISFSFPARFAA"
     gene            complement(7114..7860)
                     /locus_tag="PLO70_08120"
     CDS             complement(7114..7860)
                     /locus_tag="PLO70_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ74469.1"
                     /translation="MIKAMIVDDEQSSIDLLQWLIGQYCPDITAIKTARSVQEAIPSI
                     QSFKPDVLFLDIQMPHQSGFDLLTTIDNWNFEVIFTTAYNEFAIQAIRFSALDYLLKP
                     VDESELKKAVERYKAKRIYAPAGQLLFRNFIQNISHGNKNKFKLALADTTEVKYVQLD
                     EIIRLQAESNYTHIFLTGNKVFVSAKTLKEYDEILQGHKFLRVHKSHLVNPAHIDAYD
                     RQGILMMSDGSRVEVARRKKEFLQQSLSGK"
     gene            complement(7857..11444)
                     /locus_tag="PLO70_08125"
     CDS             complement(7857..11444)
                     /locus_tag="PLO70_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ74470.1"
                     /translation="MKRTLVILSFYCFITPLMQAQPPAFYHLSTAEGLSDNNVTIAVR
                     DRNGILWIATTEGLNSFDGNRITTYHKYKHPELPDNNIERILVDTENRVWVRTNSDYI
                     TMLDEERKFHRILVGDTADPVNISGIFHTKTNGIVVLKGKQHYFQQKNNPLLFEKKET
                     PFDSLLNGTVGFTYFLDNDRVIYYRNNQLLLMDYATMKVLLQMSFPGLSGAHYINDNE
                     LIAFTSRGDVFCRISISQQKVIKEYKDIRDQYHLPITGTLRNMTRIDENRFAFTTYFA
                     GLYILDLQRETTEHWVHDPVDQRSIGGNNTFNIRYDSSGYLFVSTQTSGLHFYNLKQQ
                     QASSKPYFIDDNKQIFDGYIQSVVTDNESNVWMGAQDRLIKWNRTSDKSTFVPCLLPN
                     GMDISGNETIREVHIDEKGYLLVGTSRYGILVLNKQLKTVIQITDSIPGKKTNFPSRW
                     INAICADKNGDHWVGTLRGTCVVKKNTFEINTFNDHPLLNEISKIPCASLWKDNEGRM
                     WIGTTRGVWCYDQDKNSLLHYSTQNGLAHNTVYATNNDHSGNIYFATAGGLSILSKEG
                     KITSFNRSNGLRNDRCEGILRDEKGFLWIGNLNCIIRYDPVNKTFAVFEEGLGFSHAG
                     FRMRSSHKSKTGEMFWGSDKGLTYFFPDQLNYTTVPLRPAVNTLLAGDNIFHFTGEDA
                     IKFPYNTTTFVFNFSSGELTGDKKNQFLYQLLGFDNGWKKPVSIGQAVYSQLPPGHYT
                     FILKASRDGSNWYEARYPVEIIIAKPWWQQIWFRLLYVFLTAAVLLVIYRYYKKKKNT
                     REINKTIEYFANSSYEHSSVEDILWDISRNCISRLGFEDCVIYLADEEKKIMVQKAAY
                     GPKSPKPFEIANPIEIPFGKGIVGHVAKTGKALIVNDTSKDSRYIVDDDSRFSEITVP
                     IIHEEKVIGIIDSEHSKKRFFTKQHLQALQTIATLCSTKISRAMAMDAMKKSKLELME
                     LNVKMAESKFLNLRLQMNPHFLFNSLSSIQHLIVSQQATKAYKYLTVFSNFLRSLLNY
                     ADKNFIPLDAELKILQMYIELESLRFDQSFSYEIKADESLSNDEVLVPSLMVQPFAEN
                     AIWHGLLHKEGDKKLSIRFSNNSEEYLTCIIEDNGIGRQKAETIQKAKITAMVHESKG
                     ISIIKERLELLQQKTGKPASLEIKDMYINNEATGTKVIITIPYYNPEES"
     gene            complement(11466..12929)
                     /locus_tag="PLO70_08130"
     CDS             complement(11466..12929)
                     /locus_tag="PLO70_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74471.1"
                     /translation="MKKIVLLAAFITNIAQAQNVGIGTDMPASKLTINGTDPDIGLMN
                     NGLAHSFIKALGFNLNIGTASDNPTGKLILGTKDNNHFNIDHLGRVSIGTNSNFDASF
                     KINGTSPIFGFLHEEVQKGFIRLAGDNFKLGTYPFNSGKIVFSPKNVDKIWIDEDGLM
                     GIGTATPVSELTINGTNPYIQIQNGASNKGFLQAVGNNLKLGTNSTNTTGSLIMQTKL
                     LDRMTIDENGQVGIGTTNPSSILTINSTDPILQLRSDDVDKGFLQLVGADIKLGTNIN
                     NDYGRTIFRNNGTDRMVINYDGKVGIGTMFPFQTLSLNATTPSLGLSIGESLYGSISV
                     DNTSKDLVIEKSTLGSGKIIINASGGTGWGVHMTEDNYFHYGGGLNPAGYPFSSQSRI
                     LAPDFVALAVNSWPDYVFANDYKLRPLTEVKKFIEINKHLPNIPSAAQIEKEGIQLGD
                     MSKRLIEKVEELTLYILQQQEQIDELKKLVQSQKKND"
     gene            complement(13076..14401)
                     /gene="ahcY"
                     /locus_tag="PLO70_08135"
     CDS             complement(13076..14401)
                     /gene="ahcY"
                     /locus_tag="PLO70_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQZ74472.1"
                     /translation="MSTVTKSIDFSLPHKVADISLAEWGRKEIRLAEAEMPGLMSIRA
                     EYGPSQPLKGARVAGCLHMTIQTAVLIETLVALGAEVKWSSCNIFSTQDHAAAAIAAA
                     GIGVFAWKGQTQEEADWCIEQTLFFGGKDKPLNMILDDGGDLTNMVLDTYPELVQHVK
                     GISEETTTGVHRLYERVAKGTLPMPAINVNDSVTKSKFDNKYGCKESLVDSIRRATDV
                     MLAGKVAVVGGYGDVGKGSAASLAGAGCRVIVTEIDPICALQAAMDGFEVKKMIDAVK
                     EADIVVTASGCRDLIRGEHFKLMKDKAIVCNIGHFDIEIDIAWLNTNYGHTKDTIKPQ
                     VDIYNVDGHDVIILAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIELWLNHKNYENKV
                     YVLPKHLDEKVARLHLAKIGVVLDELTTEQAEYLGISKEGPFKAEHYRY"
     gene            14607..14906
                     /locus_tag="PLO70_08140"
     CDS             14607..14906
                     /locus_tag="PLO70_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74473.1"
                     /translation="MIAYDTVSEAVNGLKQRGFVVDFNLEENCLVCHGDKFDINDFEI
                     VEVFRFEGNTDPSDEAIVYAIESVKGTKGILVSGYGISAEGMSAEMAKKLSLHRD"
     gene            complement(14903..15394)
                     /locus_tag="PLO70_08145"
     CDS             complement(14903..15394)
                     /locus_tag="PLO70_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74474.1"
                     /translation="MLKIRNASIKDIPLIQELTYQVWPQTYAPILSNEQIEYMLEMMY
                     SDTSLQQQMRDGARFLIAYDDKVPVGFAAYQEIKPADFKLHKIYVLRTWQGKGTGRFM
                     INHIIEEIRKLGASTLQLQVNRHNKAKDFYELLGFTIIEEADFDIGNGYFMNDYVMEK
                     KIN"
     gene            complement(15387..16118)
                     /locus_tag="PLO70_08150"
     CDS             complement(15387..16118)
                     /locus_tag="PLO70_08150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HQZ74475.1"
                     /translation="MQYIAIALAPGIAICLFIFYKDVYNREPRFNLLISFLLGCMAIL
                     PAIIFEQAFEYTIDGTITGVAIFSYAVVAFSEEFSKFLGLRLYSYSRKSFDEPLDGIV
                     YSVMVGMGFATLENLMYVLKFAEMGRGMEVGISRMFLSVPAHGTFAVIMGYFIGKAKF
                     NPGRRFLLMTAGILGAIFFHGTFDFFLFINKYSFAGKDLSEVLLAGGAIASFIVSLVL
                     SRKLIRQHQQLSRQMFNPENKITNA"
     gene            complement(16204..18618)
                     /locus_tag="PLO70_08155"
     CDS             complement(16204..18618)
                     /locus_tag="PLO70_08155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQZ74476.1"
                     /translation="MKKAVRIFWRLFIGGFAAFILLIVLANFGVFGKMPSIYDLQNPS
                     ILQASEVYAQDGTLMGKYYTERGNRSNVKYRDISRHVIDALVATEDERFYSHSGIDFK
                     STMRAVFTLGTEGGGSTITQQLALNLFNERASNRAIRVVQKLKEWIIAVKLEKNFTKE
                     EIITLYLNAVPFGNNIYGIRNASRTFFQKEPDRLNVEEAALLIGMLKGNSIYNPVRNP
                     KAALDRRNTVIGQMEVNGKITSAEAVRVKALPIKLNYKKLDENTGYAPYFREILKDEI
                     KEALKDVEKPDGGTYTIYNDGLKIYTTINIQMQQYAEEALAQQMTMLQKGINARSDMK
                     NGTVWKGHEKLLEKAIKESDRWKNLEEDGLSDKEIRASFNVKVPMKVFAWNNKREKDT
                     SMTPLDSIKYHLQMEQAGFMAMDPVTGEVRAWVGGISFKTYKNDHVNLKTKRQVGSTI
                     KPLLYTQAMEERGFTPQTECENVAQFFPGSGWVPAGKQCGGGSISMADALAFSKNCAT
                     AYIMKQVGPKQFSEFLSRLNIPTPVQPYPSIALGTCDLSMYEMLWAYTIFAGRGFSTK
                     PYAISRIEDRNGNVIKRFDYSVNRKEAVSEVTAYTMARMMQGTVDKGTAKFMRANVGA
                     AEMGGKTGTTDDNADAWFIGYTPQLLAGSWVGFEYTFMRNQGDGSRIARPITEIFFKK
                     VLADKKSGIEKDAKFIKPAELENEINSADIIISDSDPTPGAEGLDQGVGTEEDYQINN
                     NDYIGPESTPVKEDDNKTPAKKDTVSKAAVRKDDNETKPIGAAADDPKKKKGLLKRLF
                     DKKEKN"
     gene            complement(18778..21711)
                     /locus_tag="PLO70_08160"
     CDS             complement(18778..21711)
                     /locus_tag="PLO70_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74477.1"
                     /translation="MRPTRYIITAIFFLILFQASFPAFSQLGFDLNIKKPKEYEERVL
                     RSEKSDQKKFTVPRRFIQNTVTHYNYTFNANNKLNEVLERAKTAFVDDFSKLLPFYNY
                     SLDVTIRDSIQLDSITYKASSGIALHDLRNDWVDNLYLLWGAAYYLQKEFDSAYQMFQ
                     FMNYAFAPKEKDGYYKTIGSSRDGNSALSIATKEKNSIPRRLLSQPPSRNDAFIWQIR
                     NFLAQDQFPEAASLIVTLRNDPVFPKRLVNDLHEVQAYWFYKQNMWDSAATHLEQALS
                     NATNQQERARWEYLAGQLFELAGNYKDAEKNYGRAISRTTDPILEIYARLSTIRSNKD
                     GGEKSIEKNVAELLKMARRDKYTDYRDIIYYMAAQMQLEGNNVESAMALLLKSTQTAS
                     NNPGQRNKAFLQLAELSFNRKEYRSSYNYYDSLQIDDPSIENPEEISSRKKSLEVIVT
                     NLETIERQDSLQRIAALPEDERRDFVKKMVRQIRKQQGLKDEQPLTSGLRPLNNQPPT
                     TLFPTGDSKGEWYFYNATSRSRGQAEFKAKWGSRPNSDNWRRSAALLNSKLAQQTSQQ
                     QGDNSKSSGDASSGTSYEDLYAKLPVTPEQLQQSNDSIQKAMLALGKAYIQGIEDCQA
                     GTTTLEQLRNRFPEFMPMEEVLFNLYYCYNKNGETEKAAAISKLMKEKYLDSDLTTIV
                     TTGKNPRDASANTEATKTYEKIYDLFIEGNFNEAISQKKIADSVYGKNFWTPQLLYIE
                     AVYYIKQREDSTAKNVLNSIISQFANTPLANKATGLLDVLSRRAQIEEELRNLVVTRN
                     DDSNTTRPVTTVIPAKPAPKPDSITKKPPTVVVPVKPMPDSINNKPALKVIPPYAFKA
                     TDKYYVVLVLDKVDPVFSNEAKNAFFRYNRETYYNKTYTVELFQLDDDNRLMLMSSFN
                     DAQDAVNYVEKTRPKTATEIIPWLKGGKYTFTIITESNLQLLKTSKDMEAYKNFLEQH
                     LPGKF"
BASE COUNT         5897 a         4401 c         4575 g         6886 t
ORIGIN      
        1 gctcaacatg acaaggattt tcagctttag cttgctgcgt ttgtcatgtt tcggcaggct
       61 caacatgaca gtttaatata gcaggctcaa catgacaagc ttaagatagc aagttcaata
      121 caacaagaac tgtctgcctg agcttgccga aggctgtcta ataaatgata tcagttgact
      181 ataactcttt ccaggtatga tcagccaata gctgaacggc cgcaacaaat cttttgaagg
      241 ggcccgattt tccccattct ttaggagata ctaatgacag gatgaacgtt tcatcctgtt
      301 tttcgtagag gtaataggtt tgcccgatat tgggtttgaa agacattttt gcgctataga
      361 tcgtcagcga caggtctttc cttttttgaa tctcatgtgc ctgtaatgcc agcagctcta
      421 tttgtttgcg gatctggtcc agctgcatat tggtctgctc ttccatcgcc gtcagcgcct
      481 tgtgtttgat aatgccttct tcattcgctt ttatcacggc acctgaaaca gaagcagaat
      541 agggcagtac actcaattgc ttatggtata tttccatatt ggtataggaa ctaccatctt
      601 ctttaatgcc ctgttgcgtt atccgtaaat agccattcgg cattatctca tgtaccacat
      661 gcagggctat ctctcctttc ttgtagaaat gaatttcaaa ggtgatgcca tccggtattg
      721 ctgcctgaac tttatcggcc agttcatgat tgtcaaattc attcaggtcg ccatcggcat
      781 ttattttata agcggaggca aaggcctggt tttccagcgt tacccagtta tgataaatgg
      841 ccagtacttt ttggtcggga acggcttcaa tcttataaat accacttttc ggtctttcac
      901 cgtattcgta ggttcctttt tcagggaaca attgccagga ggctaaaaaa ttatatgcct
      961 gtaccataag agatgtgcat aattacaaaa tttgagctaa aaagttttac ctggccagaa
     1021 atagtagcac ttattttaaa ataaggctgt agcattattc aattatcttt atttttcttt
     1081 aaatcttatt gatgaagagc tatttcggtt cctatttcat atctcccggt gctgagcctg
     1141 ttgaatccag tgtgcttgtc tttgataaaa agatgagtat tgggtttcgc aaccccgatg
     1201 gatccaatgg tattgttcat tggccgctta cggaggtggt agtctcctat gaactttcaa
     1261 cacaacgaac aaaggtccgt cataccgggc tgggtactga attgtacatg gaaggaaagg
     1321 aggcggctgc ttttataaag gaaatgcagg atgacctgca aaggccctgg cataaaaaga
     1381 ataattcaaa agactgggga aggaatctat tgctgttatc tggatttttg ggtggcctgg
     1441 tattgcttta ttttctgatc gtaccctggc ttgcagaaaa aatggcttcc actgtttcgg
     1501 tgaaaaccga aaggcaatta ggtgatgctg tatatgatgc gatggggtta tctgcagcag
     1561 aagatacagc tgccagtttt ttgctgaatg aattttttgc aacgatggag gtacccagtg
     1621 cttacagtgt gcggataacg gtggtcaatg ataatgtggt caacgctttt gctgtaccgg
     1681 gagggaggat agtggtatac agtgctttat taaaacaaat tcaatcttac ccggaactgg
     1741 ctgccttgtt aagtcatgag ttcactcatg tgaacagcaa gcattccact aaatctattt
     1801 tcaggcgatt aggatcaaaa gtatttatcg ggttgttatt tggcagtttt ggttctgtta
     1861 cttctgtgct gatcgatcat gcggatgacc tgaaatctct cacttattcc aggaaactgg
     1921 aaaaggaggc cgatataaag ggtttagaaa tactcctgca gcgaaaaatt gatccgaaag
     1981 gatttgaaga tctgtttcat catttaaaag aggcttcccc tgcttcggca ttgccggagt
     2041 tcttaggcag tcatcccgat attgaaaaaa ggatcagtta tattaaagaa tcgtcaaaaa
     2101 atgcaaccat agttgtggat cagcgattac aatctatttt tgaaaaaata aaacaataaa
     2161 caaatgctta ttacaacaac caatgtcgta gacggacgac ctgtacagga atacctggga
     2221 gtgatcaatg cgcaaagtat catcggtgca aatattttta aagacatctt tgccggtctg
     2281 cgtgatgtat tcgggggtag aagtaaaacc tatgaaaaag tattggagca ggcaaaggaa
     2341 gatgcgttaa gagagttggc tgaaaaggca caggcgctgg gggcaaatgg tattcttggg
     2401 gttgacctgg attttgaaac catcggcagt ggaggtagta tgctgatggt gatcgcaacc
     2461 ggaactgcag ttcgtctgta acgattgcag gattaatagt gtaaagtatt attttatgaa
     2521 acgacttttg ttttctggat tcttatgtgt attactgtcg ttgcaggtga tgagccagca
     2581 aacaactgtt cctgcggcag cagcaataat actgaaacag gcttaccgtg aagcatcgga
     2641 acaaaataag aatgtattag taatgttcca tgcttcctgg tgtggctggt gtcaccggat
     2701 ggataccagt atcaatgatc cggtctgtaa aaaattcttt acggataact atgttatctg
     2761 ccacctggtg gtcgatgaat cggctgaaaa aaagaaatta gaaaatccgg gagcggttga
     2821 attacgaacg aaatatcatg gtgacggaca gggtatacca ttctggctga tttttgataa
     2881 ggaaggccgg ctactggcag attcaaagat cagaacagcg gggcaaggac ctgaagaagg
     2941 tgagaatgtg ggttgcccgg ccagcgaaaa agaagtgtct tactttatca gtgttttaaa
     3001 aatgacctca ccgctaaatg atcagcaatt agaagtaata cgaaaacggt tcagggaaaa
     3061 tgaaaaataa ttgtgtcagc ggatgagcgt agcgacccct ttttgaaaat aggctttgcc
     3121 ggtataatct gttgccttta ctgaccatac aaatgtgccg gtggcctgta actggccatt
     3181 tatttttcca tcccatcctt gtccgttgac ctgggtggaa aaaagaagct gtccccagcg
     3241 attgtacaca ttgaagtatt caatactttt cattcctaca gcaatcggta gcaatagatc
     3301 attcttgccg tcattattag gcgtgaaacc ggttggtaca aaaacggttg gccctgtttt
     3361 aaatactttg atcgttatgt aagcagaatc tttacatcct tcttcgttgt agccgatcag
     3421 tttatacctg aaactggcag actcatcatt gaatatcgca acagggttgg gaatattgct
     3481 ggcagaaaga ttatcaggtg gtaaccagct ataagccgca gcgccggaag catttaactg
     3541 caggggttgt cctgcgatta ctgctgtgtc agctccggca taaacgttca tttttggcag
     3601 caccgttact ttaattgtat cacttacagg tttagggcag ccttttgtat ctgtaacagt
     3661 aagtatataa tctgttgttc ggggtggaaa ggcgattgga ttcaatatac tggcgttact
     3721 cagccggtta gcaggcctcc atgaccagga actgccatct gtgattccgt tcaatagtaa
     3781 agatgcattg ttacagatag aaacatcttt gccggcatcc accacaggat agggaacagg
     3841 tgttacggta atattttcaa tggcggagca tccgccaatt atagcagtga cctggtaact
     3901 ggtaactgtg ttggtaataa cgaaaggatt ttttactgcc ggattgataa tttgtcctcc
     3961 gggtgtccat acatattgca aagcatccga tatgatcttc agctgaattg tatcaccttt
     4021 acaaatagta gtatcattca ttgcctggag gctcactgca ttcaccactc ttatcagcac
     4081 cgagtctttg ttcacgcaac cgctttcatt cagttcaaca taatatttcg tagtgacagt
     4141 aggagaaaca gacggcattg ccgtgttggg attagtgata tttaccagcg gtgaccagct
     4201 gaagttcccg gttccgcctg cctgcaatag cagcatatca ttacggcaaa taagtgtatc
     4261 acggaaagcc agttgtattg gtggtttatc tatgatcgtt accggtttgg taattgtatc
     4321 ccggcaacct cttgtatcag ttacgatcag tcttgcctgt ttgatacccg tctgcggata
     4381 ttggtaggaa ggattttgca gggaagaaaa atcattactg ctgcttgttt ctccaaaatc
     4441 ccatgtccat gaattgggag tgccgtatac agatgtggtc tggtcagtaa aaagggtggg
     4501 attagttata caaatgcctg acgaagtaaa accgggtgta aagcccggga aaaccctgat
     4561 gagggcagtt gcagaatctg aacaggtttg attacggtta ataacaagtt tgactgtata
     4621 tatcccgata gccggaaaag tgtaggttgc aatcgggtct gctgatgtat aaataatcgt
     4681 attagtttga tctgagaact cccattccgt tgaaacgatc aacggactgt tggattgatt
     4741 tgcaatggtt atgggttgag tgttcttaca taacaggtat tccggctgca atgatgctgc
     4801 agcaatatcg caatctgcaa tattgatttg aatatctttt cgttgccggg caattacaat
     4861 tccatttctt atttcctcta cgcaaacagt tactacgtag gcgccaacat caggggcaat
     4921 acctgttatt aatccagttg ccgcattgat ctgaacagca cctccaagtg gttgtgattc
     4981 ataaaagtca gggtaattat agggtaccgg gggaaagggt ggtggttctg gtggtaatgc
     5041 ggtgccacca cccggtgctg tactgttata ggcttcgcag aaagaatagc gaagagaatc
     5101 gccatctgta tcctgtgcag caaaactata gctgaaatta ttattggcgc aaactacaac
     5161 gaggtcgttg ccggtaaaaa aggcactgtt atttaccgga gcggtaatat tagaaatgtt
     5221 acccggtata tctgccgtat aagtggcccc tacattgtta tacccggacg atagattatt
     5281 gataccactg atccggtaat tgacatgact tgccagtata tatcccgcgg cagatgccgg
     5341 taaggttatg gcaaaattgt aataggcaac ttcatagcag acattcggtg gattcgtgat
     5401 acagggatcg ggattagtaa tacttattgt ttcctggtta cctattgtaa cagagatatc
     5461 tgttacccgg gaattattgg ttttattaaa aatagaaata atagcaggat taggaaactg
     5521 gcggccactt ccgcaacgct gaaataattt tagggttaca ttatagctgt attgtccgtt
     5581 aatcattcct gtaaacgtat agaacatttc gccaccagtt atatgactgc tcctggctga
     5641 aaaagaaaag ccagcaaaaa acattgatat gataaaaaac cttttcatga tgagccgaag
     5701 aaaggaataa aatggacttt ggttttgccg gaattggacc cttcttaaag ttaagtatat
     5761 aatcgatagg cataatcacc cggttgccat ctttggggca ggggatacta ctccttattt
     5821 taacaaactg ctttcaggct gaaaatcaat taatacttat ttttaatggg taatactatt
     5881 cccatgaagt tattattaac aattgtctta ttgctgcctt tgtttagcta tgcacaaaat
     5941 tgtaaactga acaaggagac agacccttac acaaaagaga caaaactatc caccgggttt
     6001 atcattttta gcggtgggtc ggtaaccatt gatgcagaca gcaaagaggt ggatatgctt
     6061 ttttccattg aaggggcaaa taggtgtttt gataataatt ccactgctgt ggttttcttt
     6121 gaaggcagta aagcgaaaat gaatttacgc aatgccggta caatgaattg tgaagggctt
     6181 tttcatttca tttataaaaa ttctgtaaac actacttctc agctacaaaa actgacaact
     6241 cagaaaataa gccagatcgt tttcaccgga aatgataaaa aagagattaa gattatactt
     6301 tctccgcaaa accaggaggc attagttgcg ttgggcacct gcctggtaaa tgaggcaaaa
     6361 ggtcttataa agtagaatta tccggccgca cagcaaaaac acttagtccg attacagaaa
     6421 acttttaaag tctgaaaaat cttaaagggt attggtcttg ctattggcac ctgtcctaaa
     6481 tttagtatgg tttaaaagta ttaccgtttt ttgagaacgg gtatatctct attgaaaaca
     6541 ccgatcaaat tgcaacccta tggaaaagta tcagccaggt actccgcttg ttttacatac
     6601 cggaatttca ttacagcaac tggtttgtaa ccttattgac agatcccggc ctgttgccct
     6661 tcataacaga actcatgttg tgaaccaggt gggcagggaa cttgtccttg gatttgacaa
     6721 tgaaaatgtt gtttctgtta taagggatct gttatctgcc attattgaga attcctgcga
     6781 cggggaaata tatatctggg cagaaaaata cagtgatgtc attatgctgc tgattgaaga
     6841 aagaaataat aataaaggct ttgctttgtc attcagtata ggctcaatag aaccgtatgc
     6901 agcaaggatt ggtggacata tctctattga aggaatcagg caacggaaga cgcttatatc
     6961 atttagtttt cctgcccggt ttgcagcata aaattttaag tacctgtagt gttctgcttc
     7021 agtgggtact tccttgttaa ttttatgctg atctgccctc agcggtggaa gccatcgtta
     7081 tgtttttcat accgtttttt tacagcctaa acattattta cctgaaagag actgttgcag
     7141 gaactctttt tttctccggg ccacttccac ccgggaacca tcactcatca ttagaattcc
     7201 ctgccggtcg taggcatcta tatgtgccgg gttgacgaga tgagatttat gtacccgcag
     7261 aaatttatgt ccctgtaaaa tttcatcata ctccttcagt gtttttgcag agacaaacac
     7321 tttgttaccg gttagaaaga tatgggtata attgctttct gcctgcagcc ggatgatctc
     7381 atccagttgt acatacttca cttctgtggt atctgcaagt gccagcttga atttattttt
     7441 gttgccatgt gatatattct gaataaaatt tcggaacaag agttgtccgg caggagcata
     7501 gatccgcttg gctttatatc gttcaactgc tttttttaat tcagactcat caaccggctt
     7561 taacaggtaa tccaatgcac tgaatcgaat ggcctgtatg gcaaattcat tataggcggt
     7621 agtgaagatt acttcaaaat tccagttgtc gatggtcgta agcaggtcaa atccgctttg
     7681 atgcggcatc tgtatatcta aaaacagcac atccggtttg aatgactgta tagatgggat
     7741 cgcttcctga acgctgcggg ctgtttttat agctgtaatg tccgggcagt actgcccgat
     7801 cagccattgc agcaggtcaa tcgaagattg ctcatcatct actatcattg cttttatcat
     7861 gactcttcag gattgtaata gggtatggta atgatcacct tcgtgccggt tgcttcattg
     7921 tttatataca tatcttttat ttcgaggcta gcaggtttcc cggttttttg ctgcagtaat
     7981 tccagtcttt cttttatgat gctaattcct ttgctttcat gcaccattgc ggtgatcttg
     8041 gctttctgta tggtttcagc tttttgacgg ccaatgccat tatcttcgat tatacaggtc
     8101 aggtattcct cggaattatt ggagaagcgg atggacaatt tcttgtctcc ttccttatga
     8161 agcaggccat gccagattgc attttctgca aaaggctgta ccatgagtga tggaaccagc
     8221 acttcatcgt tgctcaggct ttcatcagct tttatttcat aactaaatga ctggtcgaaa
     8281 cggagcgatt ccagctcaat atacatctgg agtatcttca gttcagcatc taaaggaatg
     8341 aagtttttat cagcatagtt tagcagggaa cgtaggaaat tggagaaaac ggtcaggtat
     8401 ttataagctt tggtagcctg ttgcgatacg atcaggtgct gaatggagct taaagaatta
     8461 aaaagaaaat gagggttcat ctgcaggcgg agattgagaa actttgattc ggccatcttc
     8521 acatttagct ccatcagttc cagtttactt tttttcattg catccattgc cattgccctt
     8581 gaaattttgg ttgaacacag cgttgcgatc gtttgcaatg cctgtaaatg ttgttttgta
     8641 aagaagcgtt ttttgctatg ctctgaatca atgattccga ttactttttc ttcatggatg
     8701 ataggtactg tgatctccga aaagcggctg tcatcatcta cgatgtaacg gctgtctttt
     8761 gaggtatcat ttacgatgag cgccttacct gtttttgcta catgacccac aatgccttta
     8821 ccaaaaggta tttcaatcgg gttggcaatt tcaaatggtt taggactttt tggtccgtag
     8881 gctgctttct gaaccattat tttcttttcc tcatctgcca ggtagatcac acaatcttca
     8941 aatcccagtc gtgaaatgca gtttcgggaa atatcccaaa gtatatcctc cacagaagaa
     9001 tgttcataag aagaattggc aaagtattct atagtcttgt tgatctccct ggtatttttc
     9061 ttttttttat aatagcggta gatgacaagc agtactgctg ctgtgagaaa aacgtaaagc
     9121 agcctgaacc atatttgctg ccaccagggt tttgcaataa tgatctctac tggataacgg
     9181 gcctcatacc agttgctgcc atcacgggag gcctttaaaa taaaagtata gtggccaggg
     9241 ggaagctggc tgtatacggc ttgtccaatc gaaactggtt ttttccagcc attatcaaaa
     9301 ccaagcagct ggtacaggaa ctggtttttc ttatcgccgg tcagttcacc ggaagagaaa
     9361 ttaaaaacga aagttgttgt attgtaagga aattttatgg cgtcttctcc agtgaaatgg
     9421 aaaatattat caccagccag taatgtatta acagctggtc gtaacggtac agttgtatag
     9481 ttcaattgat cgggaaaaaa ataggtgaga cccttatccg atccccaaaa catttcgccg
     9541 gttttgcttt tgtgagaaga ccgcattctg aaaccggcat gactgaagcc taatccttct
     9601 tcaaaaacag caaacgtttt attgacagga tcataacgga ttatgcaatt caggttaccg
     9661 atccatagaa agcctttttc atcccttaat atcccttcgc accggtcatt tcgcaatccg
     9721 ttggagcggt tgaaactggt tattttccct tcttttgaca gaatagataa tccgccagcg
     9781 gtcgcaaaat aaatattccc tgaatgatcg ttattggttg catatacagt attatgggca
     9841 agcccgttct gggtactgta atgtaagagg ctgtttttgt cttggtcata acaccataca
     9901 ccacgggtcg taccgatcca catcctgcct tcattatctt tccataatga tgcacagggt
     9961 attttgctta tttcattaag taacggatga tcattgaatg tatttatttc aaatgtattt
    10021 ttttttacca cacaagtgcc acgcagcgta cccacccagt gatcgccgtt tttatcggca
    10081 catattgcat tgatccagcg ggaagggaaa tttgtttttt tacccggtat tgaatcggtt
    10141 atttgtatga ccgttttcag ttgtttatta agcaccagta ttccatacct ggaagtgcct
    10201 accaataagt agcccttctc atcaatatgt acttccctga tggtttcgtt gccactaatg
    10261 tccattccgt tgggtaaaag gcagggaaca aatgtgcttt tatcagaggt ccggttccat
    10321 tttatcaatc tgtcctgagc tcccatccat acatttgatt cattatcggt cacaacagat
    10381 tgaatatagc cgtcgaagat ttgtttattg tcgtcgataa aatagggttt gctgcttgcc
    10441 tgctgctgct tcaaattata aaaatgcagc ccggatgttt gtgtactgac aaaaaggtaa
    10501 ccgctgctgt cataccgtat attgaatgta ttattgccac ctatactgcg ttggtctacc
    10561 gggtcatgaa cccagtgctc ggtagtttct ctttgcaggt caagaatata gagtccggca
    10621 aagtaggtag tgaaggcgaa acggttttca tcaatacggg tcatattacg aagggtgccg
    10681 gtaatgggaa gatgatactg gtccctgata tccttgtatt cttttatcac tttttgctgg
    10741 ctgatactga tgcggcagaa gacatcacct cttgaagtaa aggcaataag ttcgttgtca
    10801 ttaatataat gagccccact gagtcccgga aaggacattt gcaacaatac tttcatggtt
    10861 gcataatcca tcagtagcaa ctgattatta cgatagtaaa tgaccctgtc attatcaagg
    10921 aaataggtaa agccaacagt accattaagc aaactatcaa acggtgtttc ctttttttca
    10981 aacaacaaag ggttattctt ttgctgaaaa tagtgttgct ttcctttcag taccactata
    11041 ccgttggttt tggtatggaa gattccactg atattgaccg ggtcggccgt gtcgcccacc
    11101 agtatacggt gaaattttct ttcttcatcc agcatcgtta tgtaatcgct gttggtacgt
    11161 acccacaccc tgttctccgt atccaccagt attcgttcaa tattattatc cggtagttcg
    11221 ggatgtttat atttatgata agtagtaatg cgattgccgt caaaactgtt gagcccttct
    11281 gtagtggcga tccaaaggat gccattgcgg tctcttactg caatggtcac attgttatca
    11341 ctcaatcctt ctgccgtgct taaatgataa aatgcaggcg gctgggcctg catcaatggt
    11401 gtgatgaaac aataaaagct caatatgacg agtgttcttt tcaaagcatt ttagcaagct
    11461 ataaattaat catttttctt ttgtgactga accagttttt taagttcgtc aatttgttcc
    11521 tgctgctgaa ggatgtaaag ggtcagttct tccacctttt ctatcaggcg tttgctcata
    11581 tcacctaatt gaatgccctc tttttctatc tgtgctgcgg aaggaatatt gggcaggtgt
    11641 ttgtttattt caataaactt ttttacttct gtaaggggtc ttagtttgta atcatttgca
    11701 aacacataat ctggccaact atttacagcc aatgctacaa aatctggagc cagtatcctg
    11761 ctctggctgc taaaaggata tccggccgga ttaagaccgc cgccataatg aaaataatta
    11821 tcctcagtca tatgtacacc ccagccagtt ccaccactgg cattgatgat gatcttacca
    11881 ctgccaagtg tagatttctc aattactaaa tctttggaag tattatcaac acttatagaa
    11941 ccgtataatg attctcctat gctcaaaccc agtgaaggtg ttgtcgcatt aagagataat
    12001 gtctggaaag ggaacatagt tccaattccc accttcccgt cgtaatttat taccatccta
    12061 tctgttccgt tgtttctgaa gatagtcctt ccataatcat tatttatatt ggtacccagt
    12121 ttaatatcag caccaactaa ttgcaaaaag cctttgtcca catcatcact cctgagttgc
    12181 aatatgggat cggtgctgtt tatggttagt atggatgaag gatttgttgt gccaatacct
    12241 acctggccgt tttcatctat ggtcatcctg tcaagcaatt ttgtttgcat tatcaaactg
    12301 cctgttgtat tggtggaatt ggttccaagt ttcaggttat tgccaacagc ctggagaaaa
    12361 cctttgttgg atgccccgtt ttggatctgg atataaggat ttgtgccatt gatagtgagt
    12421 tctgaaaccg gcgtagccgt tccaataccc ataagaccat cttcatcaat ccatatcttg
    12481 tcgacatttt ttggcgagaa cactatttta ccagagttaa aagggtaagt acccaattta
    12541 aaattatcac cggcaagacg gataaaacct ttttgcacct cttcatgcaa aaatccgaaa
    12601 ataggggagg tgccatttat tttaaaggat gcatcaaaat ttgaattggt gccaatagaa
    12661 acccttccga gatgatcgat gttaaagtgg ttattgtcct ttgttcctaa tatcagttta
    12721 cctgttgggt tgtcgctggc ggttccaata ttaaggttaa atccaagggc tttgataaag
    12781 ctatgtgcaa gtccattgtt cataagcccg atatccggat cggtgccatt aattgtaagt
    12841 tttgatgcgg gcatatctgt accaattcct acattttgtg cttgggctat gttggtgatg
    12901 aaagccgcca gtaatacaat ttttttcatg gtttaaaatt tgtaccatga aagtagaatg
    12961 gtattgatgt tgaaacaaaa ccaggttagt ttacggtaat ccgttcctat tctccggttt
    13021 tgacaaaaat aaaaacccgc tggttggcgg gttttttata taaagtctga tcttattaat
    13081 acctgtaatg ttcagcttta aaaggtcctt ccttgctgat tcctaagtat tctgcctgct
    13141 cggtggtcag ttcatccaat acaacaccta ttttggcgag gtgcaatcgg gccactttct
    13201 catccaggtg tttaggtaac acatatactt tgttctcata gttcttatgg tttaaccaca
    13261 attctatttg cgccaaagtc tggttgctga atgaattact cattacaaat gacggatgac
    13321 cagtagcaca acccaaattc accaggcgtc cttcagccag gatgatcaca tcatggccat
    13381 ctacattata aatatctacc tgtggtttga tcgtatcttt tgtatgacca tagttggtgt
    13441 tcagccaggc gatatcaatt tcaatatcaa aatggccaat attacaaaca atggctttat
    13501 ctttcatcag tttgaaatgt tctccgcgga tcaggtcacg gcaaccggaa gcggtaacaa
    13561 cgatatcagc ttcttttaca gcatcaatca ttttctttac ttcaaaacca tccatagctg
    13621 cctgtaatgc acagataggg tcaatttctg taacgatcac acggcaacct gcacctgcta
    13681 aagaggctgc agaaccttta cccacatccc catatccacc aacaacagca actttaccgg
    13741 ccagcattac gtcagtggca cgacggatcg aatcaaccag tgattcttta cagccgtact
    13801 tgttatcgaa tttagatttt gttacggaat catttacatt gatagccggc attggtaagg
    13861 taccttttgc cactctttca tataaacggt gtacacctgt tgtagtttct tcagagatac
    13921 cttttacatg ctgcaccagt tccgggtaag tatccagtac catattggta aggtcacctc
    13981 catcatctaa aatcatgttc aggggtttgt ctttaccacc aaagaacaaa gtttgttcta
    14041 tacaccagtc agcttcctcc tgggtttggc ctttccaggc aaatacgccg ataccggcag
    14101 cagcgattgc agccgcggca tgatcctgtg tggagaagat attacaggaa ctccatttta
    14161 cttctgctcc cagagcaact aatgtttcta ttaaaacggc agtctgtata gtcatatgaa
    14221 gacaaccggc cactcttgca cctttcaggg gttgggaagg accgtattca gcacgaatag
    14281 acattaaacc gggcatttct gcttcagcta aacggatttc tttacgtccc cattctgcaa
    14341 gggaaatatc agccactttg tgaggaaggc taaagtcaat ggatttggtt actgtagaca
    14401 tttttttgct tttattttat ggcggcaaag gtagtattaa tgtgtctaaa aatggctata
    14461 ccaacctgat tccttcgata tattttacac aacttgttct ttaacttcat agttagccta
    14521 acagggtcat gttgaatgtt taaggtcttt ataatcatgt gaattgaaca aatagagata
    14581 acttcgaatc gtaaaatccc tttttcatga tagcatatga tacagtatca gaagccgtga
    14641 atggccttaa gcaaagaggc tttgtggttg atttcaacct ggaagagaat tgccttgttt
    14701 gtcatggcga taaatttgat atcaatgact ttgagatagt tgaggttttt cgttttgagg
    14761 ggaatactga tccgtcggat gaggcaattg tgtatgccat cgagtctgtg aagggaacaa
    14821 aaggaatatt ggtcagtggg tatggtattt ctgccgaagg aatgagtgct gaaatggcca
    14881 aaaaattgag cctgcacagg gattaattga tctttttctc catcacatag tcattcataa
    14941 aatagccatt gccgatatca aaatccgctt cttcgataat agtaaatccc agtaattcat
    15001 aaaagtcttt tgctttatta tgcctgttta cctgtaattg aagggtactg gctcccagct
    15061 tcctgatttc ttcaatgatg tgattaatca tgaatctgcc tgttcctttt ccttgccagg
    15121 tgcgcaatac atatatttta tgcagcttga aatcggcagg ttttatttcc tggtatgctg
    15181 caaatccaac cggtacttta tcatcatatg caatcaaaaa tcgggcgcca tctctcattt
    15241 gctgctgcag cgatgtatca ctgtacatca tctcaagcat atactcaatc tgctcgttgg
    15301 aaagaatagg agcataggtt tgcggccata cctggtaagt gagctcctgt attaatggga
    15361 tatcctttat tgaagcatta cggatcttaa gcattggtaa ttttgttttc cgggttaaac
    15421 atttgccggg aaagttgctg gtgctgcctg atcagtttac ggctaagtac aagtgaaacg
    15481 atgaaagaag caatagcacc acctgccaat agtacttcgc tgagatcttt gcccgcaaaa
    15541 gaatatttat tgataaaaag aaagaaatca aaagtgccat gaaagaatat agcgccgaga
    15601 ataccggcag tcatcagcaa aaaacgcctg cccgggttaa attttgcttt accaatgaaa
    15661 taacccatta ttacagcgaa agttccatgt gccggcacac ttagaaacat acggctgata
    15721 ccgacttcca tgcctcttcc catttcagca aattttaaaa catacatcag gttttctaat
    15781 gtggcaaacc ccatgcctac cattacacta taaacgatcc cgtctaaagg ttcgtcaaaa
    15841 cttttgcggg aataagaata gagacgaagc ccgaggaatt tcgaaaactc ctcactaaat
    15901 gctacaactg catacgaaaa aatagctact cctgtaatgg taccatcaat tgtatattca
    15961 aaagcctgtt caaaaataat agccggaaga atggccatac aacccagcag aaagctgata
    16021 aggagattaa acctgggctc cctgttatat acatctttgt aaaagataaa tagacagata
    16081 gctattccgg gagcaagtgc tatggctatg tattgcatat attttacttg gtgttggaaa
    16141 ggtaaaaaag aaaaagggtt ttgcagaaag caaaaccctt tgaaattttt tctttgaaga
    16201 agactaattt ttttcttttt tatcgaatag ccttttcaaa agtcctttct tttttttggg
    16261 atcatctgct gcagccccaa ttggcttagt ttcattatca tcttttcgta cagcggcttt
    16321 gctcacagta tcctttttgg caggagtttt attatcgtct tcttttaccg gtgttgattc
    16381 tggcccgata tagtcattat tatttatttg gtaatcctct tcggtaccaa caccctggtc
    16441 caggccttct gcaccggggg taggatctga atcactgata ataatatcag cactattgat
    16501 ctcattttcc agttcagctg gctttataaa ttttgcatct ttctcaattc ctgatttttt
    16561 atcggcaagt acttttttaa agaaaatctc agtgattggc cgggctatcc ttgaaccgtc
    16621 accctggttg cgcataaatg tatattcaaa acccacccat gagccggcga gaagttgcgg
    16681 agtgtagcca atgaaccagg catcggcatt atcgtctgta gttccggttt ttccacccat
    16741 ttcagcagca cccacattag ccctcataaa ctttgcagtg cctttgtcaa cagtaccctg
    16801 catcatacgg gccatggtat aagctgtaac ttcacttacc gcttccttcc tgtttacact
    16861 ataatcaaaa cgtttaataa cattaccatt tctatcttca atgcgactga ttgcatatgg
    16921 tttggtggag aaaccacgac ctgcaaaaat ggtataggcc catagcattt catacattga
    16981 caaatcacag gtgcctaaag caatagaggg gtagggttgt acaggggtcg gtatattaag
    17041 acgggataga aactctgaaa attgttttgg accaacctgt ttcataatgt aagctgtggc
    17101 acagtttttt gagaaagcta atgcatcagc catcgaaatg cttccacctc cgcattgttt
    17161 tcctgcaggc acccaaccac tgccgggaaa gaactgggct acattttcgc attctgtttg
    17221 cggagtaaaa cccctttctt ccatggcttg tgtataaagc aatggcttaa tagtagaacc
    17281 cacctgtctt tttgttttca ggttgacgtg atcgttctta taggtcttaa aactgatccc
    17341 accaacccag gctcttactt caccggtgac gggatccatc gccatgaaac ctgcctgctc
    17401 catttgcaaa tgatacttga ttgagtcgag aggtgtcatg gaggtatcct tttcccgctt
    17461 attattccag gcaaaaacct tcatcggcac ttttacatta aatgatgccc tgatttcctt
    17521 atcacttaaa ccatcttctt cgaggttctt ccagcggtca gattctttga tggctttttc
    17581 caatagtttt tcatggccct tccagactgt accatttttc atatcagacc ttgcattgat
    17641 acctttttgc agcatggtca tttgctgggc caaagcctct tctgcatatt gctgcatctg
    17701 tatattaata gtcgtataga tttttaagcc atcattataa atagtgtagg tacctccatc
    17761 aggtttctca acatctttga gtgcttcttt aatctcatct tttaaaattt cccggaagta
    17821 gggggcataa ccggtgttct catccagttt tttgtaatta agtttaatag gtaatgcttt
    17881 aacccgtacg gcctcagcag aagtgatttt cccatttact tccatttgtc ctattactgt
    17941 atttctgcga tcaagggctg ctttcggatt tcttaccggg ttatagatac tgttcccttt
    18001 cagcatacca attagcaacg ctgcttcttc cacattcaac ctgtccggct ctttttgaaa
    18061 aaatgtcctt gatgcattgc gaatgccata gatattattg ccgaacggaa ctgcattaag
    18121 atagagggta atgatctctt ccttggtaaa attcttctcc aattttacgg cgatgatcca
    18181 ttcttttaat ttctgtacaa cacggatagc cctgtttgat gccctctcat tgaaaaggtt
    18241 caatgccagc tgctgtgtta tggtactgcc tccgccttct gtccccaagg taaaaactgc
    18301 ccgcatcgtg gatttaaaat caatcccgct atggctgtag aatctttcat cttccgttgc
    18361 aaccagggcg tctattacgt ggcgggagat atcacgatat ttaacattgc tccggtttcc
    18421 cctttcggta tagtattttc ccatcagtgt cccatcttgt gcatatactt cagaggcctg
    18481 gagtatagaa gggttttgca ggtcataaat tgatggcatt ttaccgaata ctccaaaatt
    18541 ggccagcacg atcaaaagga taaatgccgc aaatccacca ataaaaaggc gccagaatat
    18601 tcggaccgct tttttcatat gttcttgaat aatggttgtg gttaaaaacg aagtttcaat
    18661 ttatgaaatc gaataccgaa aataagaaaa acctttccgg ccatgttaca ggaccattta
    18721 aatatttgcg aaagtctgct aattcgtttt aattttttaa cagattagaa agataactta
    18781 gaatttaccc ggtaaatgct gctccaggaa atttttataa gcttccatat ccttactggt
    18841 tttcaataat tgcaggttgc tttcagtgat aatggtgaaa gtatatttcc cgcctttcag
    18901 ccagggaatt atctctgtgg ctgttttagg gcgggtcttt tcaacataat tgacggcatc
    18961 ctgagcatcg ttgaaggaag acatcagcat caaccggtta tcatcatcca gttggaacaa
    19021 ttcaaccgta tacgttttgt tataatatgt ttcccggttg taccggaaga aggcattctt
    19081 tgcttcgttg gagaaaacag ggtccacttt atcaagcact aataccacat aatatttatc
    19141 tgttgcttta aaggcatagg gtggtattac tttaagtgcc ggtttattat tgatgctgtc
    19201 cggcatgggt ttgacaggaa ctacaactgt tggtggtttc ttggtgatac tatccggctt
    19261 tggagcaggt ttggccggaa taacagtagt tactggcctg gtagtatttg aatcatcatt
    19321 acgggtgact accaggtttc tcagttcttc ttcaatttgt gcccttctgc ttaaaacatc
    19381 taataatcct gttgctttgt tcgcaagtgg agtgttggca aattgggaga tgatggagtt
    19441 taatacattt tttgcagtgc tgtcctctct ttgtttaata tagtaaacag cctctatata
    19501 aagcagctgt ggtgtccaga aattcttacc ataaacgcta tctgcaattt tcttttgtga
    19561 aatagcctcg ttgaaattgc cttcaataaa caggtcataa attttttcgt aggtctttgt
    19621 cgcttccgta tttgctgatg catcacgggg attttttccg gtggtcacga tagttgttag
    19681 atcgctgtca agatattttt ctttcatcag tttactgatg gcagcagctt tttcagtttc
    19741 cccgttttta ttgtagcaat aatataagtt gaataatacc tcttccatcg gcataaattc
    19801 cgggaaacgg ttccttaatt gttccaatgt tgtagtgcct gcctggcaat cttcaattcc
    19861 ctggatatag gcttttccca gtgcaagcat agctttctgt atagaatcgt ttgattgttg
    19921 cagttgttcg ggagtgaccg gaagtttagc atacagatct tcatagctgg tacctgagga
    19981 ggcatcacct gatgatttac tattgtcacc ttgttgctga ctggtttgct gggccagttt
    20041 actgttcagt aatgcagcgc ttcttctcca gttgtcacta ttcgggcggc tgccccattt
    20101 ggctttaaac tccgcctggc ctctgctacg ggaagtagca ttataaaaat accattcacc
    20161 ttttgaatct ccggttggaa aaagcgttgt tggtggctgg ttgttgaggg gtcgcagtcc
    20221 gcttgtcaga ggttgttcat ctttcaagcc ttgttgttta cggatttgcc ttaccatttt
    20281 ctttacaaaa tcacggcgtt catcttcggg aagggcggct atccgttgca ggctgtcttg
    20341 tctttctatt gtttcaagat tggtaacaat cacttcgagt gatttttttc tgctactgat
    20401 ctcttccggg ttttctattg aagggtcatc aatttgcagg ctatcataat aattataaga
    20461 agacctgtat tctttgcggt taaaagagag ctcagccaat tgcaagaaag ctttattccg
    20521 ttgcccggga ttattgctgg ctgtttgggt acttttcagc aataaggcca tagcgctttc
    20581 tacattattt ccttccagtt gcatctgggc cgccatgtaa tagatgatgt cacgataatc
    20641 cgtgtattta tcccgcctag ccatcttcag cagctcggcc acattttttt caatactctt
    20701 ttcaccacca tctttgttcg atctgatggt agaaagcctt gcatatattt caaggattgg
    20761 gtctgtggtc cggctgattg ccctgccgta attcttttct gcatctttat aattgccggc
    20821 taattcgaag agttgcccgg cgaggtattc ccatcttgct ctttcctgtt ggttggtagc
    20881 attactcaat gcctgttcta aatgagtagc agcgctatcc cacatgtttt gtttataaaa
    20941 ccaataagcc tgtacttcat gcaggtcatt gactaatcgt tttggaaaaa caggatcgtt
    21001 cctcagcgtg acaattaaac tggccgcttc cggaaactgg tcttgcgcca ggaagtttct
    21061 gatctgccag ataaatgcat cattgcggct gggcggctgg gataataatc ttcttgggat
    21121 actgtttttt tccttggtag cgatacttaa tgctgaattc ccatctcttg aacttccaat
    21181 ggttttgtaa tacccatctt tctctttggg tgcaaaagca tagttcatga actgaaacat
    21241 ctggtaagcg gaatcaaatt ccttttgcag gtaataagcc gctccccaaa gcaggtaaag
    21301 gttatcaacc cagtcattcc gaaggtcatg caaagcaata cctgaggagg ctttgtaagt
    21361 tatagaatcc agttgaatgg aatcccggat tgttacatcc agcgaatagt tatagaaagg
    21421 gagcaatttt gaaaaatcgt caacaaatgc agttttagcc ctttccagca cttcgttcag
    21481 cttattattg gcattgaagg tatagttgta gtgggtaacg gtattttgaa taaaacggcg
    21541 gggtacagta aattttttct gatccgattt ttcggaacgt aacactcttt cttcgtattc
    21601 cttgggtttt tttatattca gatcaaatcc caactggctg aaagcgggga aactggcctg
    21661 aaaaaggata aggaagaaaa tcgctgtaat gatatatcgg gtgggtcgca ttttccgtcg
    21721 caatctaaag tcttattaac tgaatttcaa ataatttag
//
LOCUS       DAORTI010000048        21492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2024_length_21492_cov_8.651630, whole genome shotgun sequence.
ACCESSION   DAORTI010000048 DAORTI010000000
VERSION     DAORTI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21492
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2024_length_21492_cov_8.651630"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLO70_08165"
     CDS             <1..532
                     /locus_tag="PLO70_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQZ74478.1"
                     /translation="RPLPYNEWSLDFLADYIVNTHHSYVVKNLPDIRAYATKVMKVHG
                     SRHPELVPIWKLVEEVYTEMMSHMVKEEKVLFPYIKELVAANNKLQPVQASHFGTVQN
                     PINMMEMEHEVVGKCLAEIRELTSNYTLPEDACASYSLLYRMLDEFEEDLHMHVHLEN
                     NILFPKAVELEKNLNK"
     gene            623..1090
                     /locus_tag="PLO70_08170"
     CDS             623..1090
                     /locus_tag="PLO70_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74479.1"
                     /translation="MKKLFALPVIGMMLLSCNEQNHNHKHDKAEAAAVEKQHQETGPE
                     NLELNNGQKWKADSSTNQNVIFLLTVIDKFTAGTDKSLPAYGITAAELQGGLDKMISE
                     CRMKGPDHDALHKWLEPLMDAVATLKQSATESDASIQLKKIDTQVRRYAQYFE"
     gene            1090..1902
                     /locus_tag="PLO70_08175"
     CDS             1090..1902
                     /locus_tag="PLO70_08175"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQZ74480.1"
                     /translation="MKTINANTKIAFLIKQHPDALDAIVSLSPKFEKLRNPILRKLMA
                     GRASIAMASKIGGCSVSDFFVKLKDLGFEVEKNGQPEKEKAMLEMPDFLKNINPAKIV
                     KLDVRPVIEGGNDPLQNILEKIKTLQPGEVLEIVNTFEPTPLIFLLKKQGFDSYVEVI
                     NENLIQTYFYRVKETSPTGDLSMNVKSSGWEEVLQRYSNRLQTVDVRQLQMPLPMMTI
                     LEAVDKLPEGNALFVYHKRIPVFLLPELAERKLDYRINEVCEGEVHLLIFKP"
     gene            1904..>2944
                     /locus_tag="PLO70_08180"
     CDS             1904..>2944
                     /locus_tag="PLO70_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HQZ74481.1"
                     /translation="MLAGLNKDTLVKSTTHKVVLPFYLYAAISLLAATVMLCFSAEAF
                     TRHYFHPHILAVTHIMALGWGTMMILGASHQLVPVLIEHSLYSNKLAYTSFLLAATGI
                     PLLAYGFYVFDMGWPAKWGGRLILLSILSYVINLLISISKSNGKNVHAIFILTASIWL
                     FLTAFLGLALVYNFTFPYLPKETLQYLPLHAHAGVVGWFLLLVMGVGSRLIPMFLISK
                     YSNKYLLWVIYVLVNGGLLSFLGLFLYFPGSTYHLFPVLTVLIAFVLFGHYCLRSYNL
                     RIRRQVDEQMKISILSVLLMLLPFVFLVSITGWLLSDKLNTQLVLIYGFIIFFGWITL
                     IIFGMTFKTLPFI"
     assembly_gap    2945..3044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3045..3514
                     /locus_tag="PLO70_08185"
     CDS             <3045..3514
                     /locus_tag="PLO70_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQZ74482.1"
                     /translation="FGRRKTIFKQIIMLSQTCKTAIKAVIYLAARFESGEKAGIKEIA
                     DIIEASEHTVGKMLQVLVKEDVIKSLKGPNGGFFISAKQKNQPVINIVEAIDGNELFK
                     QCGLGLSKCSSSHPCPIHDEYKMVRDLFEKMCRSNTVNDLCAPVNKGLAYLIG"
     gene            3642..5450
                     /locus_tag="PLO70_08190"
     CDS             3642..5450
                     /locus_tag="PLO70_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74483.1"
                     /translation="MNTFSPHTFHIPVMGLAYTIDSPVKVARFGISSVISIVEDRLVE
                     MMRSYYYPETGRSYHPITIDQPDYRARRITDYLNLVNSIVREQVEKIKKTAFEKGSEI
                     VTYFEMLPEQSVLKQLYRQMTETEDQAEKRRLENYLRNQVTPGSIDVNIMTKIDRDNL
                     DKDNLIIKDGSDAVASLRGYINSDLTNSSIVFSAGMNPRLYNYLEQCSQFDMNEDGTF
                     SKKIILKVSDFRSALIQGKYLAKKGIWVSEFRIESGLNCGGHAFATDGFLLGPILEEF
                     KTGKQELIDILFELYSAAQLKKNNKQLTTAPPLRFSVQGGIGTAAEDKFLHSHYQMET
                     TGWGSPFLLVPEATTVDDETLKLLTNAKEEDVVLSRNSPLGVRFHYLKGTSSDKEKQL
                     RIKEGRPGSPCTEKHLALNKEFNNELLCTASRKYQQLKIRELKSSNLPEPEYQLKLAD
                     VLDKECLCVGLSNAASLKYSVPFLDKLKSVNVCPGPNIAHFSKVVSLRTMVDHIYGRS
                     VTLTPDDRPHMFINELSLYIDYLKEKMEEDKRNNQFDKRRKYYDEFYLNLRKGIQYYR
                     QLASEMEENTERFLQLLGWAETELDVLNYKYSIDYN"
     gene            complement(5504..6886)
                     /locus_tag="PLO70_08195"
     CDS             complement(5504..6886)
                     /locus_tag="PLO70_08195"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HQZ74484.1"
                     /translation="MKHIIRKSWAEPYKIKMVELLKMTTPAHRRKAMLEAGFNTFLLK
                     SEDVYIDLLTDSGTSAMSDQQWAGMMLGDEAYAGSRNFYHLEEVVKEIYGYKYLVPTH
                     QGRGAENILSKILIKKGDIIPGNMYFTTTRLHQELAGGKFEDVIIDEAHDPGNEFPFK
                     GNVDLEKLEKLIRKYGANKIPYVSMATSVNMAGGQPISMKNLKELRALTKKHGIRVIH
                     DMTRVAENAHFIQQREKGYQNKSIKAIVKEICSYTDGATMSAKKDALVNIGGFLAIND
                     WDVFEEARNMVVVYEGLHTYGGLAGRDMEAMAIGIKESVNDNHIHARVGQVIYLGEKM
                     IEYGVPIVKPIGGHGIFVDAKKILPHISQDQFPAQTLAAEVYLDSGVRTMERGVVSAG
                     RKANGENYYPKLELVRFTIPRRVYTQAHMDVIAESTARVFERRNKIKGLKMVYEPKYL
                     RFFQARFQPL"
     gene            complement(6897..8270)
                     /locus_tag="PLO70_08200"
     CDS             complement(6897..8270)
                     /locus_tag="PLO70_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQZ74485.1"
                     /translation="MKTIIEPFRIKSVEPIYFTTKEERKTILEKANYNPFRIKAKDVL
                     IDLLTDSGTSAMSSNQWAGIMQGDESYAGSSSFFKFEDSVRSITGMPYIIPTHQGRAA
                     EKIIFTILGGKGKYFISNTLFDTTRANIEFSGAEGIDLICEEGKHPTIPALFKGNIDI
                     EALKKTIREKGAENIPMIIITVTNNSGGGQPVSMQNIKDARAVCKEYNIPLFIDACRF
                     AENSYFIKLREEGYANKTVPEIANELFSYADGCTMSAKKDAFANIGGFLAMRDLDLAI
                     RCRNLLIITEGFTTYGGLAGRDLEAIAIGLQEVMEENYLQYRIRSIEYLTNKLIAAGV
                     PVMQPAGGHAVYIDAKAFLPHIPVEQYPGQSLVCALFEEGGIRSVEIGSLMFGKYDEA
                     GKLLPAQLELVRLAIPRRVYTQSHIDYVSEVIIEVFNNRNKIKGLKITEETPMLRHFT
                     AKLNPII"
     gene            complement(8448..8903)
                     /locus_tag="PLO70_08205"
     CDS             complement(8448..8903)
                     /locus_tag="PLO70_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQZ74486.1"
                     /translation="MEVKNLFEPAVYTEITERVNRLSPESQRQWGKMTVSQMLAHCKE
                     AFKVPLSEKRLPKLYPFALIGWMVKNKLYNDTPWKQGLPTAPNFIIKDERDFESEKKQ
                     LLELIKKFNEDGPAKVGLHPHPVFGKFTSEQWGKSMWKHLDHHLKQFGA"
     gene            complement(8959..9996)
                     /gene="hypE"
                     /locus_tag="PLO70_08210"
     CDS             complement(8959..9996)
                     /gene="hypE"
                     /locus_tag="PLO70_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HQZ74487.1"
                     /translation="MELQCPMPKFDFDVITLGHGSGGLLTHKLLQSGVFDIFKNELLN
                     QQHDGASFSLNGRVAFSTDSYVITPAFFPGGNIGELAINGTVNDLAMCGAIPQYLSLS
                     FIIEEGLTMKEFWEILVHIKEATQKAKIKIVTGDTKVVEKGKGDKIFINTSGVGLIHP
                     KANIHHNNIRPGDKIIISGNIATHGIAIMSVRKGLEFETTIESDTTNLNHTIEKLLDR
                     FGNDIKFLRDPTRGGVASVLNEIAELTSLGFELDQKSIPVDEQVEGACEMLGLDPLYV
                     ANEGLFVAVVAKEIADELLSLLRYDENGATAAIIGEVNTEHPGKVLMKSRIGGRRVVN
                     YLTGEQLPRIC"
     gene            complement(10001..11107)
                     /gene="hypD"
                     /locus_tag="PLO70_08215"
     CDS             complement(10001..11107)
                     /gene="hypD"
                     /locus_tag="PLO70_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQZ74488.1"
                     /translation="MKYLSEYRDPELVKQYLDQIHHITTKPWKLMEICGGQTHSLVKN
                     GILDMLPDKITMIHGPGCPVCVTPVSIIDEAIYLAQQPHVIICSFGDMLRVPGSKKSL
                     LEVKAEGADVRILYSPLEAVKLAKENPGKEVIFFAVGFETTAPANALSIIQADKSGVS
                     NYSILASHVLVPPAMEALLSDEENQIDAFLAAGHVCTIMGMEEYYPIAEKYKTPIVVT
                     GFEPVDLLQGILMALEQLEHGEYKVENQYTRYVQREGNRLAIETMQEVFSVSDRMWRG
                     IGTIPQSGFEVNHRYKHYNARLKFKLDIPVAEENKDCISGDIMKGLKKPFQCPNFGTF
                     CKPEHPLGAPMVSSEGACAAYYHYSSVNTPEETL"
     gene            complement(11104..11370)
                     /locus_tag="PLO70_08220"
     CDS             complement(11104..11370)
                     /locus_tag="PLO70_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQZ74489.1"
                     /translation="MCLAIPGKLLSISGQRDETFRQGKVSFGGITKEVNLSMVPDAQV
                     GDYVLVHVGVAIGKVDQEEARQTFEYLKMMGEVDEIVNNEKTDK"
     gene            complement(11387..13702)
                     /gene="hypF"
                     /locus_tag="PLO70_08225"
     CDS             complement(11387..13702)
                     /gene="hypF"
                     /locus_tag="PLO70_08225"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HQZ74490.1"
                     /translation="MNTYHIHIGGLVQGVGFRPFVCRLARGMDINGWVSNGNDGVHIE
                     FNATKEQADIFYTRLVSTPPSNAIIRTHHLTEVTSKLFDNFKIHPSTNTTKPDLLLTP
                     DIAICEHCRKEITDAGDKRYQYPFTTCLECGPRYSIITALPYDRKNTTMAELKMCTAC
                     EAEYSDIYNRRHYSQTNSCPDCSIPIHLYDNNGIEISNDPESILLIIKESLTHGHILA
                     VKGIGGYLLVCDATNALSIETLRDRKHRPAKPFAVMYPSLSMMEKDLVITAKEKEALS
                     GNTAPIVLCKLKEETGTGICTSIIAPGLDKIGVMLPYTPLLFLIMEQWNKPLIATSGN
                     LSGSPIIYTDEDALLWLTDYADFILSFERDIVTPQDDSVIQFSEGSQQSIILRRSRGL
                     APNYFPSPLKKSSTLLAMGAELKSAFAISDNDNLYISQYLGDQGNFDSQTSYGYTLDH
                     LLQLLQTKPAQILVDSHPNYFVSQKGKELAEEWNIPVSEIQHHKAHFAAVLAENNLLN
                     KNEQVLGVVWDGTGFGEDAQVWGGEFFKLEEGEIDRYMHLDYFPQLLGDKMSKEPRVS
                     AVSLLRNSMDHLMIIRDLFSIPEREYYLKLLQQKQNLLTSSMGRLLDGIAALLKIQSF
                     NSYEGEATMKLEAVARSCKSKSYEYYSIPVNKNRLEWAAMIEGIIMDIKEKKDTAFIA
                     RKVFMSLAMLVKNVAIRSGFRKISFSGGVFQNAFLVDLVIELLQNKFELYFHKQLSPN
                     DECIGFGQLAYETLQSENKTGLKEELQITNH"
     gene            complement(13733..14512)
                     /gene="hypB"
                     /locus_tag="PLO70_08230"
     CDS             complement(13733..14512)
                     /gene="hypB"
                     /locus_tag="PLO70_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQZ74491.1"
                     /translation="MCATCGCDANTSTSIKQIGETHHHAHHHEHDHSHSHSPGKTIIE
                     VEKDVLQENNLLAERNRGYFDAKNILALNLVSSPGSGKTSLLERTLIDLKDRFPFYVI
                     EGDQQTSRDADRIHATGTKVAQINTGKGCHLDAHMILHAVQGMKPIENSILFIENVGN
                     LVCPAMFDLGEKERVVIISVTEGDDKPIKYPDMFHTSTLCIINKTDLLPYVNFNMDKA
                     KEYARQVNPALQFIELSCTSGEGLSNWYQWLKEKILVPENV"
     gene            complement(14606..14803)
                     /locus_tag="PLO70_08235"
     CDS             complement(14606..14803)
                     /locus_tag="PLO70_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74492.1"
                     /translation="MKKQVLMIAALLSGLAGFAHEGHGHTHDFTITHYIIEPEHAFPV
                     IAAVAIVILLIKGYRRSLIKK"
     gene            complement(15057..17813)
                     /gene="ppdK"
                     /locus_tag="PLO70_08240"
     CDS             complement(15057..17813)
                     /gene="ppdK"
                     /locus_tag="PLO70_08240"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQZ74493.1"
                     /translation="MATKTKTGKYVYFFGGGKADGNESMKNLLGGKGANLAEMAGHPR
                     LRLPVPPGFTVTTEVCTYFYKNRKTYPKVLNAQVQEAMSKMEKLTGKKFGDVTNPLLV
                     SIRSGARRSMPGMMETVLNVGLNENTIKGIIAQSGDERFAYDAYRRLIMMYADVVMEK
                     GGGIEVKNGKGIRKILDEKLEHIKHKQGYSSDTELTAAELKELVKDYKKTVKKVLGTE
                     FPDDPWEQMWGGIGAVFSSWNGKRAIEYRRIERIPDEWGTAVNVQAMVFGNMGEDCCT
                     GVAFTRNPGNGENKFYGEYLVNAQGEDVVAGIRTPAPVNEYSMNEQSKHYTSLEKLMP
                     ALYKELFSYQRRLEQHYKDMQDIEFTIEKGKLYMLQCRVGKRNGVAAVRMATEMHKEK
                     LIDAKTAIMRVGPNQLVELLLPMLDPKAELIAPVIAKGLPAGPGGAKGRVVFSSAEAV
                     EWASRGEKVILVREETSPEDVDGMHKSQAILTTKGGMTSHAALVARGWGKCCIVGCAD
                     IEIHTDGKNFHAKNGVVIKEGDWISLNGTKGLVYEGSMALVDIDIDKNQSYKDLMKLV
                     DKTKQIGVRANAETPRDAITAVKFGAEGIGLFRTEHMFYGEGSEEPLFQLRKMIVSKT
                     EKERREALNGLFKYVKKDVKDTLRVMNGHPVTIRLLDPPLHEFVPHDKEKLKQLSKEL
                     GISMGMLNRRVLALHENNPMLGHRGVRLGISYPEITEMQVRAIFEAAAELNKAGKKTF
                     PEIMVPVTVIAHELRHQKVIVDRVYKEVCEKMKVKKIPYLYGTMIETPRAALRAEFMA
                     NVADFFSFGTNDLTQMSFGFSRDDIGGFLPKYLDLKILPDDPFQTIDQEGIGQLIKVG
                     TERGRVTKPNLKVGICGEHGGDPESVKFCHRIGMNYVSCSPFRVPIARLAAAQAALET
                     PRKK"
     gene            complement(17976..18362)
                     /locus_tag="PLO70_08245"
     CDS             complement(17976..18362)
                     /locus_tag="PLO70_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74494.1"
                     /translation="MALNKNHEFEELDGVKCGIVEKNVKPERVAFLKNLLEYNGYTVV
                     AVPSPAPKAMPAPKPADGEAAAPPPPPPPTPETFTVGVTDYTFNTTNAIYGRMLKTKD
                     GHIVTMAYWLQKEAVSHDEIPYYEHK"
     gene            complement(18416..19822)
                     /locus_tag="PLO70_08250"
     CDS             complement(18416..19822)
                     /locus_tag="PLO70_08250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQZ74495.1"
                     /translation="MNEFLTLMKSELVVTAIIFLLLFIKIGKGMKNDSLLSLVQVLLL
                     VNFIAGFFLNSTGNLFGDMFYTNPLIAFQKNILSLGVYLISLLCADWLKKTQHLAEFF
                     MLMLSALLGMFLMISSNNLLIFYLALELATIPVAAMANFDLDKKRSSEGAMKMILSSA
                     FSSGILLFGISLLYGTTGTISFPEIAAQLDGSSVQILAFVFLFTAFAFKLSIVPFHLW
                     TADVYEGSPIAVTSFLSVISKGSVAFIFITVLYKVFQPMQDVWYTMLVILAVATMVIG
                     NLFALRQQNIKRFLAFSSIAQVGFILVGISSNDYMGITAVVYFVLIYVFSNLAAFGVA
                     SVISEHSGKENIDEYKGLYKTNPFLSWILALALFSLAGIPPTAGFFGKLFLLTAGGAK
                     AAYWFITVAALNMIISLYYYLRVVRAMFMDKNDNPVGKIKISPSVKLALLICGAGIVL
                     IGLLSWVYDHIHTLSFKL"
     gene            complement(19834..21312)
                     /locus_tag="PLO70_08255"
     CDS             complement(19834..21312)
                     /locus_tag="PLO70_08255"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQZ74496.1"
                     /translation="MNLLLLIGAPLLTAVAVLLSRNKAQVKWISLVGSVAQLALAFAL
                     LYAFRQERAQNNMSQMLFELNYKLFDSLNINFHFGVDGISVAMILLTSFVVIAGVLVS
                     WNIEKMTKEFYFLLILLALGAYGFFISLDLFILFFFLEISVIPKYLLIGIWGSGKKEY
                     SANKLALMLMGGSALILVGMLGLYFTAGHSFDIMTLSKTSIPANIQNLLFPLLFVGFG
                     VFTALFPLHTWVPDGHSSAPTAGSMFLAGISMKLGGYGCLRIATYLLPEGAKEYANII
                     IILSAIAILYGAFATMMQKDLKYINAYSSVSHVGFSLLGIGMLTQTAITGALMQMVSH
                     GLMTALFFAVIGMIYDRTHTRMTNEMGGLMKVMPFITTVFFIVGLCSLGLPGLSGFVA
                     EMTVFMGSWENSDAFHRIATIAAAMSIVVTAVYILRAIGQTAMGPITNTSHLQLKDAA
                     WNEKLAAIILVAGIIAIGIAPGWLNDLLRPAAEEIMNRIAGK"
BASE COUNT         6048 a         4488 c         4327 g         6529 t
ORIGIN      
        1 ccgcccgttg ccctacaacg aatggagcct ggattttctg gcagattaca ttgtaaacac
       61 gcatcatagt tatgttgtaa aaaacctccc agatatcagg gcatatgcca caaaagtgat
      121 gaaggtccat ggaagccggc atcctgaatt ggtgccaata tggaaattgg ttgaagaggt
      181 ttatacggaa atgatgtctc atatggtgaa ggaggaaaag gtattgttcc cttatataaa
      241 ggaactggta gctgctaata ataaattaca acccgtgcag gcttcacatt ttgggacagt
      301 acaaaatcct attaacatga tggagatgga acatgaggtg gtgggtaaat gcctggcaga
      361 aataagagag ctaaccagta actatacatt accggaggat gcctgtgcca gttacagttt
      421 gctctatcgg atgcttgacg agtttgaaga ggacctgcac atgcatgtgc acctggaaaa
      481 taatattttg tttccgaaag ctgtggaact ggagaaaaat ttgaataaat agcatggttc
      541 cggggcgaaa tacctgatct caagggttgt ttaagagagt tacagagagg ggtttctccc
      601 ggaattaaat ttaaaatata atatgaaaaa attatttgct ctgccggtaa taggcatgat
      661 gttattaagt tgtaatgagc aaaatcataa tcataaacac gacaaggcag aagcagccgc
      721 tgtggaaaag caacaccagg aaaccggccc ggaaaatctt gaattgaata atggccagaa
      781 atggaaagct gatagtagca ccaaccaaaa cgttattttt ttgcttacgg tcattgataa
      841 gttcactgca ggtactgaca aatcactgcc ggcgtacgga attactgcag cagaattgca
      901 gggtggcctt gataagatga ttagtgaatg ccggatgaaa gggcctgatc atgatgcgct
      961 gcataaatgg ctggaacccc tgatggatgc agtagcaaca ttgaagcaat ctgcaacaga
     1021 gtcggacgca agtatccagc taaaaaaaat agacacacag gtaagacgct atgcgcaata
     1081 ttttgaatga tgaagacaat taatgctaat accaaaattg ctttcttaat aaaacagcac
     1141 ccggatgcac tggatgcaat tgtcagtctt agcccgaagt ttgagaaact aaggaatcct
     1201 attctcagga aattaatggc gggcagggcc agcattgcta tggcttctaa aatcgggggc
     1261 tgtagcgttt ctgatttttt tgttaaactt aaagaccttg gatttgaagt agaaaaaaac
     1321 gggcagcctg aaaaagaaaa agcaatgtta gagatgcctg attttttaaa gaatattaac
     1381 ccggcgaaaa ttgtcaagct ggatgttcgg ccggtaatag aaggtggtaa tgatccgctg
     1441 cagaatattt tggagaaaat taagacactg caacctggtg aggtacttga aattgttaat
     1501 acatttgagc ccactccttt gatattccta ttgaaaaaac agggttttga ttcttacgtt
     1561 gaagtcataa acgaaaacct cattcaaact tatttttaca gggtaaaaga aacatctccg
     1621 actggggatc taagtatgaa tgttaaatct tcgggatggg aagaggtatt gcaacgatat
     1681 agtaaccggt tgcagactgt agatgtgcga cagctgcaaa tgccattgcc gatgatgaca
     1741 atcctggaag cagtagacaa attaccggag ggtaatgctt tatttgttta tcataaacgc
     1801 attcctgtat ttttattacc cgaattggct gaaaggaagc tggactaccg tatcaatgag
     1861 gtatgtgaag gagaagtgca tttactgatc tttaagccct gaaatgttag caggcctcaa
     1921 caaggatact ttagttaaaa gcacaacaca caaagtggtg ttgccttttt atttatatgc
     1981 tgcaatatcc ctgctcgcag ctacagtaat gttgtgcttt tcagcggagg catttacccg
     2041 gcattatttt catccacata tcctggctgt tacacatatc atggcactag gatggggtac
     2101 catgatgata ctcggtgcaa gccatcagtt agtgcctgta ttgatcgaac atagtttata
     2161 tagtaacaag ctggcctata cttctttttt actggctgca accggaattc cgttgctggc
     2221 ctatggtttt tatgtatttg atatgggctg gcctgccaaa tggggcggaa ggttaatatt
     2281 gttatctata ctatcttatg taattaatct tttaataagt atttcaaaaa gtaatggaaa
     2341 aaatgtacat gccattttta tacttactgc ttcaatttgg ttatttctaa ctgcatttct
     2401 gggattagca ttggtatata attttacttt tccatatttg cctaaagaga ctttgcagta
     2461 tttgccttta catgctcatg ccggtgttgt cgggtggttc ctgttattgg tgatgggtgt
     2521 gggatcaaga ttgataccca tgttcctgat atcaaaatac agtaataaat atcttttatg
     2581 ggttatttat gttctggtaa atggagggtt gttatcattt ttagggttgt tcctttattt
     2641 ccctggttcg acttatcatc ttttccctgt cctgacagtt ttaatagcct ttgtgctgtt
     2701 cggccattac tgcttaagaa gctacaacct tcgcatccgc agacaggtag atgagcaaat
     2761 gaagatttct attttgtctg tactactgat gctgctgccg tttgtttttc ttgtaagtat
     2821 aactggatgg ctattgtcag acaaattgaa cacccagctg gtacttatct atggtttcat
     2881 tatttttttt ggctggatca ccctgatcat ttttggaatg acatttaaga cattaccttt
     2941 tattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttcg gaagaaggaa
     3061 aacaattttt aaacagataa ttatgttaag ccagacctgt aaaacagcta ttaaagctgt
     3121 tatctacctt gctgcccgct ttgagtcagg agaaaaagcc ggtataaaag aaattgctga
     3181 tataatagaa gccagtgaac atacggttgg gaaaatgctg caggtactgg taaaagaaga
     3241 tgtgataaaa agcctgaaag gaccaaatgg tggatttttt atatcggcaa agcaaaagaa
     3301 tcagccggtt atcaatattg ttgaagctat cgacggaaat gaattgttta aacagtgcgg
     3361 tctcggtttg agtaaatgtt catccagcca tccttgcccg attcatgatg agtataagat
     3421 ggtacgggac cttttcgaaa aaatgtgcag aagcaatact gtaaacgatt tgtgtgcgcc
     3481 ggttaataaa ggattagctt acttaattgg ataatagcat aaaaactact cgtacttaaa
     3541 gcgccggcag gatgctgttg atcctataaa ataattaaac gggagatgag cctcatttta
     3601 ttgctgaaca tcttttttaa tattataaat tttatattat tatgaataca ttctcgcctc
     3661 atacttttca tatccccgta atgggattgg catatactat tgacagtccg gtgaaagtcg
     3721 cccgttttgg tatttcctcc gttatttcaa tcgtagagga tcggctggtg gaaatgatgc
     3781 gcagttatta ttatcctgag acaggccgtt cctatcatcc gattactata gatcagcctg
     3841 attaccgggc gaggcggatt accgattacc ttaaccttgt aaattccatt gtcagggaac
     3901 aggttgagaa aataaaaaaa actgcttttg aaaaggggtc tgaaatcgtt acctattttg
     3961 aaatgctgcc tgagcaatct gtgttaaagc aattataccg ccaaatgacc gaaacagagg
     4021 accaggctga gaaaaggaga ttggaaaatt atctccgtaa ccaggtcaca cccggcagta
     4081 ttgatgtaaa cataatgacc aagattgatc gtgataattt agataaggat aaccttataa
     4141 taaaagatgg ctcagatgca gtggcttctt tgagagggta cattaacagc gatcttacca
     4201 attcttcgat tgtattttct gcgggaatga accccaggtt atataattat ttggaacagt
     4261 gcagtcagtt tgatatgaat gaggatggga ctttttcaaa aaaaattatt ttgaaagtca
     4321 gtgacttccg ctctgcactg atccagggca aataccttgc aaaaaagggg atatgggtga
     4381 gtgagttcag gattgaatcc ggtttaaatt gcggcggtca tgcttttgcg actgatgggt
     4441 ttttactggg cccgatcctg gaagaattta aaactggtaa gcaagagctt attgacatcc
     4501 tatttgagct ttatagtgca gcacagttga aaaaaaataa taaacaactg acaactgctc
     4561 ctccgcttag gttttcagtc cagggaggaa tcggaactgc agcagaagat aaattcctgc
     4621 acagtcacta ccagatggaa accacgggtt ggggttctcc tttcctgctg gtacctgagg
     4681 caacaactgt ggacgacgaa accttaaagc tattgacgaa tgcaaaggaa gaagatgttg
     4741 tgttgagccg taactcaccg ctcggtgtcc ggtttcacta cctgaaaggc accagttcag
     4801 acaaggaaaa gcaattacga ataaaggagg gcagaccggg aagtccctgt accgagaagc
     4861 acttggcttt aaataaagag tttaataatg aattactttg tacggcttct cgcaagtacc
     4921 agcaattaaa gatccgggaa ctgaaatcct ccaatcttcc agaaccggaa taccaattaa
     4981 aactggcaga tgtccttgac aaggagtgtt tatgcgttgg attaagtaat gctgcaagct
     5041 taaaatattc cgttccgttt ctggataagc taaaatcagt aaatgtgtgc cccggcccta
     5101 atattgccca tttttcgaaa gttgtatccc tgcgtaccat ggtggatcat atatacggaa
     5161 gatctgttac actgactccg gatgaccgcc ctcatatgtt tattaatgag ctgagtttgt
     5221 atatcgatta cctgaaagaa aaaatggagg aagacaagcg gaataaccag tttgataaaa
     5281 gaaggaaata ttatgatgag ttttacctga atctccgtaa ggggatacaa tattacagac
     5341 aacttgcctc tgaaatggaa gaaaatacag agcgttttct gcagctgctg ggatgggcag
     5401 agacggaact ggatgtgtta aactacaaat acagtataga ttataactaa atggctgttc
     5461 tttttaagga acagccggtt tacagttggt ttcgttgccg gatttagagt ggttgaaatc
     5521 ttgcctggaa gaacctcaga tactttggtt catataccat ttttaatcct ttgatcttat
     5581 tacgtcgttc aaatacccta gcggttgatt cggcaattac atccatatgt gcctgcgtgt
     5641 acacccggcg ggggatagta aatcgaacca attccagttt ggggtaataa ttttccccgt
     5701 ttgcttttct tccggctgac actacacctc tttccatcgt tctaacacca gaatcaagat
     5761 atacttcagc agctaaagtt tgtgccggga actggtcttg tgaaatatgc ggcagaattt
     5821 ttttagcgtc cacgaatatg ccatgcccgc caatcggctt tacaatgggc acgccatatt
     5881 caatcatttt ctcacctagg taaattacct gaccgaccct ggcgtgaata tggttgtcat
     5941 tcacactttc tttgataccg atcgccatgg cttccatatc acggccggcc aatccaccat
     6001 aggtatgtaa tccctcatat actactacca tgtttcgggc ttcttcaaaa acatcccagt
     6061 cgttgatggc aaggaaacca ccgatattca ccaacgcatc tttttttgca ctcatcgtag
     6121 ctccatcggt ataagaacaa atttctttca ctatggcttt aatggatttg ttttgatatc
     6181 ctttttccct ttgctgtata aaatgagcat tctccgccac cctggtcata tcatgaataa
     6241 cacggatgcc gtgttttttt gttaaggctc tgagttcttt caggtttttc atactgatag
     6301 gctggcctcc ggccatatta acactggtag ccatacttac gtacggaatt ttattggcgc
     6361 catatttcct gatcagcttt tctaattttt ccaaatccac attgcctttg aaaggaaatt
     6421 cattgccggg gtcatgagct tcatcaataa taacatcctc gaattttcca cccgccagtt
     6481 cctggtggag ccgggtagtg gtgaaataca tattgccggg aatgatatca ccttttttga
     6541 tcaggatctt tgataaaatg ttttctgcgc cacgtccctg gtgagtgggt actaaatatt
     6601 tataaccgta aatttctttt actacttctt cgaggtgata aaagttccgg ctgcctgcat
     6661 aggcttcgtc acccagcatc atacctgccc actgctgatc actcattgca gaggtgccgc
     6721 tgtcagttaa taaatcaata tatacatctt ctgatttcaa taaaaaagtg ttgaagcctg
     6781 cttccagcat ggcttttctg cgatgggcag gagttgtcat ttttaacaat tccaccattt
     6841 ttattttata gggctcggcc cagctttttc tgataatatg tttcataaat ctgtttttaa
     6901 attattgggt tgagtttagc ggtgaaatgt cttagcatcg gcgtttcttc agtaattttt
     6961 aatcctttta ttttattcct gttattaaat acttcaatga ttacttccga tacataatca
     7021 atatgacttt gggtatatac tcttctggga atggcaagac ggaccagttc cagttgtgct
     7081 ggtaataatt tccctgcctc atcatatttg ccaaacatta atgaacctat ttccacacta
     7141 cggatacccc cttcttcaaa taaagcacag accaatgatt gtccagggta ttgctcaacg
     7201 ggtatatgag gcaggaatgc ttttgcatca atatatactg catggccacc tgctggctgc
     7261 ataaccggga caccagctgc aattaatttg ttcgtcaggt actcaatact gcggatacgg
     7321 tattgcaggt aattttcttc catcacttcc tgcaatccta tagcaatagc ttcaaggtca
     7381 cgaccagcca gacctccgta cgtagtgaac ccttcggtga tgatgagcag atttctgcaa
     7441 cgtatagcta aatcaaggtc ccgcatggcc agaaaacctc caatattggc aaatgcatct
     7501 ttttttgcac tcatggtgca tccatcagca taggagaaca attcgtttgc tatttccgga
     7561 actgttttgt ttgcataacc ttcttcccgc agtttgatga agtaagaatt ttctgcaaac
     7621 cggcaggcat ctataaatag cggtatgttg tattctttac aaactgctct tgcatcttta
     7681 atattctgca tactcaccgg ttgcccgcca cctgaattgt tggtaacggt tattataatc
     7741 ataggaatat tttcagcacc tttttcccgg attgtttttt tcagggcttc tatatcaata
     7801 tttcctttaa aaagtgccgg tatagtcgga tgtttccctt cttcgcagat gagatcaata
     7861 ccttcagccc cggagaattc aatattggct cttgtggtat caaataatgt attgctgatg
     7921 aaatattttc cttttccacc gagaatagtg aaaatgattt tttcagcagc ccgtccctgg
     7981 tgagtgggaa tgatataggg cattccggta atgcttctta cggaatcttc aaatttaaaa
     8041 aagctgctgc tgccggcgta cgattcatct ccctgcataa tgcctgccca ttggttactg
     8101 ctcatggcac tggtaccact gtctgtcagc aaatcgatca gtacatcttt ggcttttatt
     8161 cgaaaggggt tatagttagc tttttctaaa attgtttttc gttcttcttt ggtggtaaaa
     8221 taaattggtt ctaccgattt tatacgaaag ggttcaatga tcgttttcat gcaagatgat
     8281 ttaaccagta ttgctactgg taattgaaaa aaataaaata atgagataac tggatttcca
     8341 gggagggaat tcaggtaggt ttactgatga tatttttcca gatgttcatg aatacaaaaa
     8401 gcctgctcaa aagtaggagg agcaggcttt ttgtaatatg actttaatca ggcgccaaac
     8461 tgtttcaggt ggtggtcgag atgcttccac atactcttgc cccattgttc tgacgtgaat
     8521 ttcccaaaaa caggatgagg gtgcagaccc actttagcag ggccatcttc attaaatttt
     8581 tttatcagct ccagtaattg ttttttttcc gattcaaaat ctctttcgtc tttaatgata
     8641 aaattggggg ctgtaggcag gccttgtttc cagggagtat cattatacaa tttgtttttt
     8701 accatccagc caatgagggc aaacgggtat agctttggca accgtttttc acttaagggt
     8761 actttgaatg cttctttgca atgggccagc atctggctaa cggtcatttt tccccattga
     8821 cgttgacttt ctggtgaaag ccggtttact ctttctgtaa tttcagtata tacggctggc
     8881 tcaaacagat ttttaacttc catgatgttc gaatttaaaa aagaaagatc ggaaaaattt
     8941 cagaaaaata gccagtgatc agcatatacg gggcaattgt tcgccggtaa gataattaac
     9001 aacccttctg cccccgatcc tgcttttcat aagcactttg cccgggtgtt cagtatttac
     9061 ttcaccaatg atagcagcag tggccccgtt ttcatcgtag cgcagcaacg acagcaattc
     9121 atcagcgatt tcttttgcta caactgctac aaataatcct tcattggcca catatagcgg
     9181 gtcaaggccc agcatctcac aggcgccttc cacctgttca tctacaggaa tggacttttg
     9241 gtccagttca aaaccgagag aagtgagctc tgcgatctca ttaagtacgg aagctacacc
     9301 accccttgtc gggtcacgta gaaattttat gtcgttacca aacctgtcaa gcagtttctc
     9361 aatagtatga ttgaggttgg ttgtatcgct ttcaatcgtt gtttcaaatt ctaatccttt
     9421 tcttacactc attatggcga taccatgtgt ggcgatattg ccgctgataa tgattttatc
     9481 gccgggccgg atattattgt gatgaatatt tgctttgggg tgaatcaagc caacgccgga
     9541 ggtgttgatg aaaatcttgt cacctttacc tttttctact actttggtat caccggttac
     9601 aattttaatt ttagcttttt gtgttgcttc ttttatatgt actaaaattt cccaaaattc
     9661 cttcattgta agaccttctt caataatgaa agaaagagac aggtattgcg gaatagcccc
     9721 gcacatagcc agatcattca ccgtcccgtt tatagctagt tcgccaatat tacctcccgg
     9781 gaaaaaagcc ggtgtgataa catagctgtc agtactgaaa gccacccgtc catttaagga
     9841 aaaactggca ccatcatgtt gctggttgag taattcattt ttgaaaatat caaacacgcc
     9901 gctttgcagc agtttgtgtg taagtaatcc gccgctgccg tgaccaagag ttatcacatc
     9961 aaaatcaaat ttgggcatgg ggcattgcag ttccatttta ttataaagtc tcctccggag
    10021 tatttactga tgaataatga taataagcgg cacaagctcc ttcactgcta accattggtg
    10081 cacccaaggg atgttccggt ttacaaaagg tgccaaaatt gggacattga aatggttttt
    10141 tcaaaccttt cataatatca ccactgatac aatccttatt ttcttcagcg acaggaatat
    10201 ccagcttaaa ttttaaacgg gcattgtagt gtttatacct gtgatttact tcaaaaccac
    10261 tttgaggtat ggttccaatt cctctccaca tcctgtcact aacggagaat acttcctgca
    10321 tggtttcaat agctaaccgg tttccttctc tttgtacata ccgggtatac tggttctcca
    10381 ctttatattc cccatgttcc aattgttcta gggccattaa aatgccctgc aaaaggtcaa
    10441 ccggttcaaa gcctgttact acgatcgggg ttttgtattt ttcagcaatc gggtaatatt
    10501 cctccatgcc cataatggta caaacatgtc ctgctgccaa aaaggcatcg atctggtttt
    10561 cttcatcact cagcaaggct tccatagcag gtggtaccag cacatgtgaa gcaagaatac
    10621 tgtaattgct aacacctgat ttatctgctt gtattatgct cagtgcattg gccggggcgg
    10681 ttgtttcaaa acctactgca aaaaatatta cttctttgcc agggttttct tttgccagtt
    10741 ttacggcttc caaaggcgaa tagagaatcc gtacgtcagc accttctgct tttacttcga
    10801 gtaaactctt tttagaaccg ggtactctca acatgtcgcc aaatgaacaa ataatgacgt
    10861 ggggttgctg tgcaagataa atagcctcat caataatact cactggtgtt acacagacag
    10921 ggcaacccgg accatgtatc atggtaattt tatccggcag catatccaga ataccatttt
    10981 ttaccaggct atgtgtttgc ccgccgcaga tctccatcag tttccatggt ttggtagtga
    11041 tgtgatggat ctggtcaagg tattgcttca ccagttcagg gtctctgtat tctgataaat
    11101 acttcattta tcagtttttt cattattgac gatttcatcc acttcaccca tcatcttcag
    11161 gtattcaaaa gtttgccggg cctcttcttg atctactttg ccgatggcca cacctacatg
    11221 caccagtaca taatcaccaa cctgtgcatc cggcaccata gaaagattta cttctttcgt
    11281 gatgccgcca aaggagacct ttccctgtct gaacgtttcg tcccgctgtc ctgaaatgga
    11341 taataatttt ccgggtattg ccaaacacat agtattcgtt tttagttcaa tgattcgtta
    11401 tttgtaattc ttcttttagc ccggttttat tttcagactg taatgtttca tatgccagct
    11461 gcccaaaccc gatgcactca tcattgggac ttagctgctt atggaaatat agttcaaatt
    11521 tattttgtaa cagttcaatc accaggtcaa ccaggaaagc gttttggaaa acaccaccac
    11581 taaatgaaat tttcctgaaa ccggagcgga ttgcgacatt tttgaccaac atagctaatg
    11641 acataaatac ttttcttgca ataaaggcgg tgtctttttt ttcttttata tccattatta
    11701 tcccttctat cattgcagcc cactcaagcc tgtttttatt gacggggata gagtagtact
    11761 cataggattt gcttttgcaa cttctggcca ctgcttccag tttcatagtg gcttctcctt
    11821 cataggaatt aaaggattga atttttagta gggccgcaat accatctaat aagcgtccca
    11881 tactggaggt aagtaaattt tgtttttgtt gcaggagctt caggtaatat tccctttcgg
    11941 gtattgaaaa caggtccctg attatcatca ggtggtccat ggaattacgc aataaggaaa
    12001 cggcactcac cctcggttct ttactcattt tatcacccag caattgaggg aaataatcaa
    12061 gatgcatata acggtcgatc tcaccctctt ccaatttaaa aaattcacct ccccagacct
    12121 gtgcatcttc accaaagccg gtaccatccc aaacaactcc cagcacctgt tcgtttttat
    12181 tcaacaggtt attttctgcc aacacagctg caaaatgggc tttatgatgt tgtatttctg
    12241 atacaggtat attccactct tccgccagtt cttttccttt ttgtgataca aaataattgg
    12301 gatgactgtc aaccagtatt tgtgctggtt tggtttgtaa aagctgtaat aaatgatcaa
    12361 gagtgtagcc gtaacttgtc tgtgaatcaa aattaccctg gtcaccgaga tactggctga
    12421 tatacaggtt atcgttatca gatatggcaa atgcactttt caactcagca cccattgcca
    12481 gcaaggttga actttttttg agcggggaag ggaaataatt aggtgccaaa cctcttgacc
    12541 ttcttagaat aatggattgc tggctgcctt cggaaaactg gatcacacta tcatcttgtg
    12601 gcgttacaat atctctttca aaactcaaaa tgaaatcagc ataatcagtc agccacagca
    12661 aggcatcttc atctgtataa ataataggcg aaccactcag gttgccgctg gtagcaatca
    12721 gcggtttgtt ccattgctcc attattaaaa agagtaaagg agtgtaagga agcattacgc
    12781 cgatcttatc aagcccgggt gcaatgatag aagtgcaaat gcctgtacca gtttcttctt
    12841 tcaatttgca taaaacaatc ggggcagtat taccggataa agcctctttt tcttttgctg
    12901 ttatcaccag gtctttttcc atcattgaaa gagatggata cattacagca aatggtttgg
    12961 cagggcgatg ctttcggtct ctaagtgttt cgatagaaag tgcattggtc gcatcacaga
    13021 ctaacaggta gccaccaatc ccttttacgg caagaatatg accatgagtc aatgactctt
    13081 ttatgatcag gagtatgctt tcaggatcat tggagatttc tataccatta ttgtcatata
    13141 aatgaatggg tatactgcaa tcgggacagg aattggtctg gctataatgc ctccggttat
    13201 aaatatctga atattcagcc tcgcaagccg tacacatttt taattccgcc atcgttgtgt
    13261 ttttccgatc atagggcagg gcggtgatga tagagtaacg ggggccgcat tccaaacagg
    13321 tagtgaacgg atattggtat cgtttatcgc cggcatctgt aatttcttta cggcaatgtt
    13381 cgcagatagc aatatcagga gtcagtaaaa gatcgggttt agtggtgtta gtacttggat
    13441 gaatcttgaa attatcaaac aacttactag taacttctgt taagtgatga gtccggataa
    13501 ttgcattgga cggaggagta cttactaacc gggtataaaa aatatccgct tgttcttttg
    13561 ttgcattgaa ttctatatgt actccatcat tgccattact tacccatcca tttatatcca
    13621 tcccccgggc aagacggcat acaaagggcc ggaaaccgac tccctgtacc aatccgccga
    13681 tatggatgtg ataagtgttc ataatagata tttagttgca tgccagccaa cgctaaacat
    13741 tttcaggaac cagtattttt tcttttagcc attgatacca gttggataat ccttcaccgc
    13801 tggtacaact tagttcgata aactgcagtg ctggatttac ctgccgggca tattcttttg
    13861 ctttatccat attgaaattg acatagggca gcagatcggt cttgttaatg atacataggg
    13921 ttgacgtatg gaacatgtca ggatacttga tgggtttgtc atcaccttct gttacgctta
    13981 tgataaccac cctttccttt tcgccgagat caaacatggc aggacataca agatttccca
    14041 cattttctat aaacaggatg gaattttcaa ttggcttcat accctgaact gcatgcagta
    14101 tcatgtgtgc atcgaggtgg cagcctttgc cggtatttat ctgtgctact ttggtacctg
    14161 ttgcatgtat ccggtcagca tctcttgaag tttgctgatc gccttcgatc acataaaaag
    14221 gaaaacggtc tttgaggtca ataagtgttc tttcgagtaa agaagtttta cctgaacccg
    14281 gagaactcac caggtttaaa gccagtatat tcttggcatc aaaataaccc ctgttgcgtt
    14341 ctgccagcag gttattctcc tgcagtacat ctttttcaac ctctatgatt gttttgccag
    14401 gagaatgcga atgggaatga tcatgttcgt ggtgatgagc atggtgatgt gtttcaccga
    14461 tttgtttaat tgatgttgaa gtattggcgt cacatccgca ggttgcacac ataaaaaaga
    14521 gtttaggttg ttgtttcaat ttattccggt gaatttattc tttcaccata aatgaagaaa
    14581 taatattgga actaatttgt ttaaatcatt tttttattag cgatctccgg tagcctttta
    14641 ttaacaatat tacaatggct accgctgcaa ttaccgggaa agcatgttct ggctctataa
    14701 tatagtgtgt tattgtaaaa tcatgcgtat gcccatgtcc ttcgtgagcg aaaccagcta
    14761 aaccggataa gagagcggct atcattaata cttgcttttt catatttcaa atttatattg
    14821 tcaatattat accagaatca ctgatttagc gctgactttc atcaaagcag aaagtgataa
    14881 ctgtcattct gcacattcaa tataaaagtc ttttaattaa tgacttataa ctcaaagtat
    14941 tctattggta tttaaaaaag attatcagcc ttgtggacac ttcaaacgac ttttgtgcaa
    15001 aacaggaaag cctgcaacag tgagttgcag gctttcctgt tttgatattt ctctgcttat
    15061 ttctttctcg gcgtttcgag ggcggcctgt gcagcagcta accgtgcaat cggcacccgg
    15121 aacggagaac aacttacata gttcatacca atgcggtggc agaacttcac actttcggga
    15181 tcaccgccat gctcaccaca gatacctact ttcaggttag gtttggtaac ccgacctctt
    15241 tcagttccta cttttatcaa ttggccgata ccttcctggt caatggtctg gaacggatca
    15301 tcaggcaata ttttcaggtc aagatatttt ggtaagaaac caccaatgtc atcccggctg
    15361 aaaccaaaac tcatctgggt aaggtcgttg gtaccaaaac tgaagaagtc ggccacgttt
    15421 gccatgaatt ctgctcttaa ggcagccctt ggtgtttcga tcatagtgcc atagaggtat
    15481 gggatttttt tcaccttcat tttttcacat acttctttat aaactctgtc cactataact
    15541 ttctggtggc gcagttcatg ggcaataaca gttaccggca ccataatttc cgggaatgtt
    15601 tttttaccag ctttgttcaa ttccgcggca gcttcaaaaa tggcccttac ctgcatctct
    15661 gtaatttcag gataactgat accaagacga acaccgcggt gacccagcat cgggttattt
    15721 tcatgcaatg ccagtaccct tctgttcaac atgcccatgc tgatgcccag ttctttgctg
    15781 agttgcttca gtttttcttt atcatgcggt acaaattcat gcaatggtgg gtccagcaga
    15841 cggatagtaa caggatgccc gttcattact ctcagtgtat ccttcacatc tttcttgacg
    15901 tatttaaaca atccgttcaa tgcttctctt ctttcttttt ctgttttact aacgatcatc
    15961 ttacgcaatt ggaacagcgg ctcttcactt ccttccccat agaacatatg ttcagtgcgg
    16021 aataagccaa taccttcagc accaaacttc acagctgtga tggcatctct tggcgtttca
    16081 gcattggctc taacaccaat ctgtttggtt ttatccacca gcttcatcag gtctttatat
    16141 gactggtttt tatcaatatc aatatctacc agtgccatgc ttccttcgta tactaaacct
    16201 tttgtaccat tcaggctgat ccagtcgcct tcttttatca caactccgtt tttagcatgg
    16261 aaatttttac catcagtgtg tatttcaata tcagcacaac ctacaataca gcacttaccc
    16321 catcctctgg caaccagtgc tgcatgggat gtcataccgc cttttgtagt aagaatagcc
    16381 tgtgatttgt gcataccatc cacatcttcc ggtgaagttt cttcacgaac aaggatcact
    16441 ttctcacccc tggatgccca ttccactgct tctgcagaag agaacaccac acggcctttg
    16501 gcaccaccgg gtcctgccgg caaacctttt gcaataacag gtgcaatcag ttctgctttg
    16561 ggatcaagca ttggtaatag cagttccact aactggttcg gacccaccct cataatagcg
    16621 gtcttcgcat caatcagttt ttctttgtgc atctctgtag ccatacgtac agccgccaca
    16681 ccatttcttt tacccacacg gcactgcagc atgtagagtt ttcccttttc aatggtaaac
    16741 tcaatatcct gcatgtcttt ataatgttgc tccagtcttc tctggtagct gaataattct
    16801 ttatataatg cgggcatcaa tttttccagc gaagtgtaat gtttgctctg ttcattcatg
    16861 gaatattcat tcacgggtgc cggagttctg ataccagcca ccacatcttc cccctgtgca
    16921 tttactaaat attccccata gaatttattt tctccattac cggggttgcg ggtaaaggcc
    16981 acacctgtgc aacagtcttc acccatattg ccaaatacca tagcttgtac attcactgca
    17041 gttccccact catcaggaat tctctcaatc cggcggtatt caatagctct tttaccgttc
    17101 cagctggaga atacggcacc gataccaccc cacatttgtt cccatggatc atccgggaat
    17161 tcagtaccta ataccttttt cactgttttt ttataatctt tcaccagttc ttttagttct
    17221 gcggcagtca gttccgtgtc actgctataa ccttgtttgt gtttgatatg ctccagtttt
    17281 tcatccagta ttttgcggat gccttttcca ttttttactt ctataccacc gcctttttcc
    17341 atcaccacat cggcatacat catgatcaat cttctgtatg catcgtatgc aaagcgttca
    17401 tcaccacttt gtgcgatgat ccctttgatg gtattttcat tcagtcccac atttaatacc
    17461 gtttccatca tacccggcat ggaacgacgg gctcctgaac gaatggaaac caataatgga
    17521 ttggtaacgt cgccaaattt tttgccggtg agtttctcca ttttggacat tgcttcctgc
    17581 acctgggcat ttaatacttt tggatatgtt ttcctgtttt tgtagaaata tgtacagact
    17641 tcagtggtaa cggtaaaacc gggaggtacc ggtaaccgaa gcctggggtg acctgccatt
    17701 tcagcaaggt tagcaccttt cccacctaat aaattcttca ttgattcgtt tccatcggct
    17761 ttgccgccgc cgaagaagta cacatacttt ccggtttttg tttttgttgc cataatataa
    17821 aaagcttttg tgcttgcata gttatgaatt gatacaggca attatgaaaa cacaggtcag
    17881 aaagaacctt gattcttatc attttgtaca aaaaggaaac tcagtaaaga ataatttact
    17941 ggcagacaaa gggctgtgtg agagaaggaa gtgagttatt tatgttcgta ataaggaatt
    18001 tcatcatggc tcacggcctc tttctgtagc cagtatgcca tagtaacgat atgcccgtcc
    18061 tttgttttca gcattcgtcc ataaatagcg ttggtggtat taaaagtata atcagtaacg
    18121 ccaaccgtaa aagtttcagg ggttggtggc gggggaggag gcggagctgc agcttctccg
    18181 tcagcaggtt ttggtgcagg cattgcttta ggtgccggtg acggaacggc taccacggta
    18241 taaccattgt attccagtaa gttttttaga aaagctactc tttccggctt cacgttcttt
    18301 tctacaatac cgcatttaac accgtccagt tcttcaaatt catgattttt attaagagcc
    18361 ataaaaaagt tattagttgc agggtttaag taataaatcc tatgttctat tttacttaga
    18421 gcttaaaact tagtgtatgt atatgatcat agacccagct aagcaaacct atcaaaacaa
    18481 tacctgcgcc acaaatgagc aatgccagtt ttacggatgg gctgatcttt atttttccaa
    18541 ccgggttgtc atttttatcc atgaacatgg ccctcacaac acgaagataa taatagagcg
    18601 aaatgatcat gttcaatgca gcaacagtaa tgaaccaata agcagctttt gcgccgccgg
    18661 ctgttaacag gaaaagctta ccaaagaaac cggctgttgg cggaataccg gccagtgaaa
    18721 ataaagctaa tgcaaggatc caactgagga aaggattggt tttgtacaac cctttatact
    18781 catcaatatt ttctttacca ctatgttctg aaatgactga tgccacacca aaagcagcaa
    18841 ggttagagaa cacatagatc agtacaaaat ataccaccgc tgtaataccc atgtaatcat
    18901 tgctgctgat accgactaat ataaagccta cctgtgcaat ggaagaaaaa gcaagaaagc
    18961 gtttgatatt ctgctggcgc agggcaaata aattaccaat gaccatagtg gcaacagcca
    19021 gtattacaag cattgtgtac cacacatcct gcatcggctg aaatactttg tacaaaacag
    19081 taataaagat gaatgccaca gaaccttttg atattaccga taagaaggat gttacagcaa
    19141 taggggagcc ttcatataca tctgctgtcc acaagtggaa cgggacaatt gataacttaa
    19201 atgcaaaagc agtaaagaga aacacgaatg ccagtatctg tacggaagac ccgtcaagct
    19261 gagcggctat ttcagggaag ctgatagtgc cggtggtgcc atagagtaag gagatgccaa
    19321 ataataaaat gccggaagaa aatgcagatg acaggatcat cttcatcgct ccttcagaag
    19381 aacgtttctt atccaggtca aaattcgcca ttgcagctac cggtattgtg gctaactcaa
    19441 gtgccaggta aaatatcagc aggttgttgc ttgagatcat cagaaacatg cccaacaatg
    19501 ctgacagcat aagcataaaa aattcagcca ggtgctgtgt tttcttcaac caatctgcac
    19561 aaagcaggga aatgagataa acaccaaggc ttaaaatatt tttctggaaa gctattaatg
    19621 ggttagtata gaacatatct ccaaagagat ttcctgtact attgagaaag aacccggcaa
    19681 taaaattaac cagcagtaaa acttgtacaa gagatagcaa tgaatcattc ttcattccct
    19741 tgccgatttt tataaatagc aggaggaaga tgatggcggt aaccaccagt tcgcttttca
    19801 ttaatgtgag aaactcattc atccaatcag gttttatttt ccagcaatcc tgttcatgat
    19861 ctcttcagca gccggtctca gcaaatcatt taaccaaccg ggtgcaattc cgatggctat
    19921 aattccggcc actaatatta tggcagccag tttttcattc caggctgcat ctttcaattg
    19981 taaatggcta gtgtttgtaa tggggcccat ggctgtttgg cctatggccc gtaaaatata
    20041 aacggccgtc accactatcg acatggctgc tgcaatggta gcgatgcggt gaaatgcatc
    20101 actattttcc caggacccca taaagacagt catttctgca acgaagccac ttaatccagg
    20161 taagcccaaa gagcaaaggc caacaataaa aaatacagta gtgataaacg gcatcacttt
    20221 catcagcccg cccatttcat ttgtcatgcg ggtatgtgtc cggtcataga tcattccgat
    20281 aacagcgaag aagagggctg tcattaaccc gtgagatacc atttgcatca atgccccggt
    20341 gatcgctgtt tgtgtcagca taccgatacc cagtaaactg aatcctacgt gactcactga
    20401 tgagtaggcg ttgatgtact tcagatcttt ctgcatcata gtggcgaaag caccatataa
    20461 gattgcaata gcagaaagaa taataataat gttggcgtat tcttttgcac cttcaggaag
    20521 caggtaagtg gctatccgta aacaaccata gccacccagt ttcattgaaa taccggccag
    20581 gaacattgaa cccgcagttg gtgcagaaga gtgaccatca ggtacccagg tatgtaaagg
    20641 gaacaatgcc gtgaacacac caaagccgac aaataataac gggaacagca ggttttgtat
    20701 gttagcaggg atactagttt ttgataaagt catgatatca aaactatgtc cggcagtaaa
    20761 atacaggcca agcatgccta ccaatattaa ggcggaacca cccatcagca tcagggccag
    20821 tttattggcg ctgtattctt ttttgccact tccccagatt ccgatcagta aatatttggg
    20881 aatcacggag atctccagga agaagaagag aataaacaga tcaagtgaaa taaagaatcc
    20941 gtaagctccc agtgccagta aaatgagcag gaagtaaaat tctttggtca tcttttcaat
    21001 gttccaggat accagtacac cagcaattac aacaaaggag gtcagcaaga tcatggctac
    21061 ggaaattccg tctacaccaa aatgaaaatt gatattgagc gaatcaaata atttataatt
    21121 cagctcaaat aacatttggg acatattatt ctgtgccctt tcctgtcgga aggcataaag
    21181 taaagcaaat gctaatgcca gttgcgcaac agagccaacg agtgaaatcc attttacctg
    21241 tgccttattg cgggaaagca atacagcgac ggcagtcagc aacggagctc ctatgagtaa
    21301 taaaagattc atcaattcgt atttatcaaa aatgtcctat ctcaatacat aaataaataa
    21361 agtcgctaaa acaatcacac ctgctaaaaa gtaaattgcg tattgctgca cttttcctga
    21421 ctgtattttt ttaattcctt cagaaacagc ttgcgttgtg ttaccggtca ggtttaccat
    21481 tccatctaca at
//
LOCUS       DAORTI010000049        21119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2080_length_21119_cov_7.499051, whole genome shotgun sequence.
ACCESSION   DAORTI010000049 DAORTI010000000
VERSION     DAORTI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21119
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_21119_cov_7.499051"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /gene="ftsZ"
                     /locus_tag="PLO70_08260"
     CDS             complement(<1..1698)
                     /gene="ftsZ"
                     /locus_tag="PLO70_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQZ74497.1"
                     /translation="MIHFDLPKEKSSILKVIGVGGGGGNAVNFMFNQQIDGVNFIICN
                     TDAQALSNSGIPNRIQLGPHLTQGLGAGANPDIGRQATEESLEEIKRILEVNTKMAFI
                     TAGMGGGTGTGGAPIIAKICKDLGILTVGIVTTPFAYEGKKRQLQAEEGIKTLKGYVD
                     TLLVISNDKLRHQFGNLKMREAFEKADNVLATAAKCITDVINSTGQINVDFADVCTVM
                     RNGGVAILGNAEAAGENRAQKAIEEALNSPLLNDNDIQGAKWILININSAQGEHEFTM
                     DEVEIIQNYLLTQAGVDTDVILGMGYDSTLGDKIGITLIATGFEHKDPFAKPIPKKEE
                     PKPEEKIVMILGQTEETPVIPINQKVEIQQALLEKEMEATKSSLEVTKKEDYLMPKLV
                     EEFPVAAPVIDLSDIKEEEPVVIHWELQSEEKLLSKITDEMISISTVSKMSTVAKDES
                     IHSVSQSESVPPTIKETDAELILSMKDSTSSTTPVSAASGGYLARPSNIYAESKPEVV
                     TSKTSAEEPVPSKLASNEQEELLELQMQIIERDDIPAADMPMAHQAQSPLSTEVEDSA
                     "
     gene            complement(1772..3145)
                     /gene="ftsA"
                     /locus_tag="PLO70_08265"
     CDS             complement(1772..3145)
                     /gene="ftsA"
                     /locus_tag="PLO70_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQZ74498.1"
                     /translation="MNNEQPIIVGLDIGTTKIAVIAGRKNEFGKLEILGFGKSNSNGV
                     KHGQVLNIDETIKAIKTALENCFASNPNLDINEVYVGIAGHHIKSLQTRGDIVRQKTE
                     EEITQREIDQLIDDQYKTYIPAGDQIIDVIPQEFTVDNFQNIPNPIGYGGVKIGANFH
                     IITGDKNAIRNINRSVEKSGLHTKDLVLQPLASSSAVMGQEDLEAGVAIVDIGGGTTD
                     LAVFYEGILKHTAVIPFAGENITNDIKTGLGVLKTQAEQMKVQFGSALANEAKANAYI
                     TIPGLRGMPAKEISVKNLANIIQARMSEIMDFVTYHLKQVGLDNKALNGGIILTGGGS
                     QLKHLIQLTEYVTGLNARIGFPNEHLAAGHIEELSKPTYSTCIGLILKGYDDYEHNRK
                     QFEKEYKKVEVPDGLRRVAEEEGEPVVEEVVSDDKVKQRKGLNNFWGKFKDGIIDLFK
                     EEEDKHL"
     assembly_gap    3241..3340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3341..4197)
                     /locus_tag="PLO70_08270"
     CDS             complement(<3341..4197)
                     /locus_tag="PLO70_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HQZ74499.1"
                     /translation="MNAKSTIRKILFVTVWLCIGGGMFTLLLAAISKRNKGLCKDYSI
                     VIKGSLNNYFIDKNDVEQQLKKASKGSIKGQPLSSLNLHELEKMLENNTWISDAELYI
                     DNQEVLHISVTEKDPVARIFNTEGYSFYIDESGMRMPLSEKLSARVPVFTGFPAKKII
                     TAIDSMLLKKLTVMANYIVNDSFWLAQVAQIDITPERTFEMIPVVGNHIVKMGEGENI
                     ERQFNRLMVFYKQVLSKTGFDKYKVIDVRYAGQVVASRYAGNSKVDSIQLKKNVEKLL
                     QQSRDAENDT"
     gene            complement(4201..5598)
                     /gene="murC"
                     /locus_tag="PLO70_08275"
     CDS             complement(4201..5598)
                     /gene="murC"
                     /locus_tag="PLO70_08275"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQZ74500.1"
                     /translation="MNTPFRGIEKMNEVYFIGIGGIGMSAIARFFHAGGVKVSGYDKT
                     PTVLTKELEASGIKVHYEENVELVPKNVDLVVYTPAIPAEHKELVYYQTNGYKVVKRS
                     DVLQIISESSFNICIAGTHGKTTITTMVAHLLRDSGFGCNAFLGGIAVNYGTNFWSSE
                     RNVCVIEADEYDRSFLKLSPDVAIITAMDADHLDIYGTAEAMEEAFIDFSKRVKLEGL
                     LVRQFGLKRGKELNADRHLTYSLQNESADVYAANIRMMNGSYEFDVMMKDNKLENVRL
                     NMGGMHNVENAVAAITVASSLGIENDKIKAAIENFRGVKRRFEYVIRNERLVFIDDYA
                     HHPEELRALINGAKTLFKQKKCTVIFQPHLYSRTRDLAEGFAQVLDLADEVILLPVYP
                     ARELPIEGVSSEMVLDKMENEHKRVLKKEELISWIQQDYARTLNKEFGEVLITAGAGD
                     IDMLVEPMKNELKDL"
     gene            complement(5630..6718)
                     /gene="murG"
                     /locus_tag="PLO70_08280"
     CDS             complement(5630..6718)
                     /gene="murG"
                     /locus_tag="PLO70_08280"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQZ74501.1"
                     /translation="MSKRVIIAGGGTGGHIFPAIAIANALKKIDGSIEILFVGAKGRM
                     EMEKVPQAGYRIEGLDIAGFNRSSLIKNIGLPYKLLKSFLQVRTIFKKFNPDAAIGVG
                     GYSSFPVLRFAQAKGVKTFIHESNSFAGKSNIMLGKKATAIFTGTDGMEKFFPARKVK
                     VTGNPVRVVIASASVTRSEGIKFFSLDEKKKTVLVVGGSLGAKSINEAIDKGLDEMLK
                     AGLQVIWQTGKPYSVKAKERAYGKQSVWVNEFITQMEYAYAAADIVVARSGAMTVAEL
                     CVAKKPVLFVPYPFAAEDHQTVNAMQLVNKKAALMVKDSEARQKLVFMTIELSLDESK
                     QEELKKNIAAQAINDADKVIAAEILKVI"
     gene            complement(6729..7994)
                     /locus_tag="PLO70_08285"
     CDS             complement(6729..7994)
                     /locus_tag="PLO70_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQZ74502.1"
                     /translation="MEATRDIRFNELKNTFNTNNLLSKTKGDKVIWALVVLLTLVSLL
                     AVYSATGSLAYKNYKGNTEIYLFKQVAFILAGIMVIYFAHLVNYTFYSRAAKIIFLLS
                     LPLLFYTLFFGVKMNEGSRWIRLPIINMTMQTSDLAKLALFMYLARLLSKKQDVIKDL
                     KKGFLPVILPVALTCALIAPANLSTALLLGASCLMLLFIGRVNTKHILMTIGIALIPI
                     AFLVAAAVIRHKSDKEEMVVAKKSSSALTARVDTWIGRVESFIYGSKDDVDNGAYQVN
                     QAKIAIAKGGVFGLGPGNSTTRDFLPQAYNDFIYAIIIEEYGLLGGAFIMFIYLVFLY
                     RCIRIFKRCPFAFGAFLALGLSFTLAIQAVANMAVTVNLFPVTGVTLPLVSMGGTSFI
                     FTCLAIGIILSVARNVEQLEGKAAADFAK"
     gene            complement(8000..9373)
                     /gene="murD"
                     /locus_tag="PLO70_08290"
     CDS             complement(8000..9373)
                     /gene="murD"
                     /locus_tag="PLO70_08290"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQZ74503.1"
                     /translation="MKNRVPYTNQNMSKRFVILGGGESGVGAALLAKKNGYEVFLSDG
                     SSLKDDYRSELKSAGIEFEEGRHTDDRILNAGEVMKSPGIPEKAEIVKKIRAKGITII
                     SEIELAYRFKGDSKIIAITGSNGKTTTTALTYHICKTAGLDCALVGNIGDSFAKQVAE
                     DPKPLYVVEVSSFQLDGIESFRPDVAILTNITEDHLDRYDYNFGNYIRSKFRITMNQQ
                     HNDYFIYCADDEVTMKYLNQFNIQSNQLPISMKRELPNGAFIKDGDMYVRTGQDFTTM
                     SVFDFALKGKHNQYNTMAACVAATTLDIRKDKIRDAVQNFQNLEHRMEHVATVRGVEF
                     INDSKATNVNSTWYALESMTKPTVLILGGVDKGNDYALIEDLVKEKVKAIICLGADNR
                     KIHEAFSTIVNPIVNTGNALEAVHAAFHFAAKGDVVLLSPACASFDLFKNYEDRGNQF
                     KQAVREL"
     gene            complement(9511..10791)
                     /gene="mraY"
                     /locus_tag="PLO70_08295"
     CDS             complement(9511..10791)
                     /gene="mraY"
                     /locus_tag="PLO70_08295"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQZ74504.1"
                     /translation="MLYHLFDWLKEEGIRFPGSELFRFITFRVLAAVLLSLFITLVYG
                     KKLIKFLQKKQISESIREEGLAGEESKRGTPTMGGIIIILAIIIPTLLLADLTNVYVQ
                     VLLISTVWLGIIGFIDDYLKLRGKRLAQQKGETYKKSGKDGLAGWFKILGQVGLGILV
                     GATLYFNSEVKIWREYVGKVPPADTTNVIAKNVNGSIKYFVPTKSVITTIPFVKNHEF
                     NYEKLLPASWRDYTWVLYILIVIFIITAVSNGANLTDGLDGLATGTSALIGTLLGIFA
                     YASGNLVFADYLNIMYIPNLGELSIFIAAMIGGCVGFLWYNSYPAQMFMGDTGSLTLG
                     GIIASLAIIVHKELLIPIFCGVFLVEALSVMLQVGYFKYTKRKFGEGRRIFLKAPLHH
                     HYQVKKYHEVKIVTRFWIVTVLLVVLSIVTLKLR"
     gene            complement(10805..>11873)
                     /locus_tag="PLO70_08300"
     CDS             complement(10805..>11873)
                     /locus_tag="PLO70_08300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQZ74505.1"
                     /translation="CGLLSTVENQIGDKVVPATHTTPDAISLNALLKQMADEGCSHVF
                     METSSHALHQHRVTGLLYAGGIFSNITHDHLDYHKTFDEYIRVKKTFFDSLPSSGFAI
                     SNADDKRGMVMLQNTNAKKYYYSLKTVADFKGKILDNSLSGLMMIVNEVEVHFRLIGE
                     FNAYNLLAVYGAAICLGEDKQEVLTALSELTGAEGRFDYQVSAKEKVIAIVDYAHTPD
                     ALLNVLATIKKLKKGFEQVITVVGCGGDRDRTKRPVMATVACEHSDKVIFTSDNPRGE
                     DPAQIIKDMEEGLAVAYKRKYISIVDRKEAIKTAISLAKAEDIVLIAGKGHEKYQEIK
                     GVRNHFDDKEVVRELFELLGK"
     assembly_gap    11874..11973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12046..14178)
                     /locus_tag="PLO70_08305"
     CDS             complement(12046..14178)
                     /locus_tag="PLO70_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQZ74506.1"
                     /translation="MEVKRDILWRVYVSFLGIVVLSLLVLGRAFYIQRVEGNHWRSMS
                     DSLHQRFIALDAQRGTIYSEDGQMLSTSIPTFDVYIDFNADGLREKKGKRFTENIDSF
                     SIALANYFGDKTAAQYKSELKLAYKKNTRYYQLKKKLSFEQYKIFREFPLARLGRNKS
                     GIIVETNSKRLPPFGLLASRTIGLSREHMASDGKIKKQNVGLERSYDTILSGKKGQKL
                     VRYIAGGTAIPIEGSETDPENGKDIFTTLDVKIQDITETALMRMMLQSEALYGTAIVM
                     ETSTGKVKAIANLGRRPDGSYWEDDNYALRATEPGSTIKLVTLLSVLEKGSSAIDDMV
                     EVGSAGRLQVGPKIVTDAERSPKPLLSVKECFAHSSNVGMSKLAYKAFGNKPQEFKEF
                     LHRFHLDVRSPIDLTDVPKPTMAPLNEKGSSLGNMLWMSFGYALQVSPLHTLTLYNAV
                     ANNGKMMKPYLVSHVQSGGILHKQFEPTIIEANICKPAVIQAARASMEAVVTEGTARR
                     VFAGVPFPIAGKTGTAQVSDGAIKYGHGVYQASFVGYFPADKPQYSCIVVVRTKPHAA
                     SHYGGTVAAPVFREIATKLYAMYVDRKDPSLFTTVKDSAAYFYAGYSTDIKKVYKALN
                     VRFTDSVAQNNWSNVYASNYQPVLKANSVRKQVMPNVRGMGLKDALYLLENMGVKVTI
                     KGKGKIATQSIAPGTSLVNGVTVVLELS"
     gene            complement(14254..14595)
                     /locus_tag="PLO70_08310"
     CDS             complement(14254..14595)
                     /locus_tag="PLO70_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HQZ74507.1"
                     /translation="MQEQQNPKERDPRLDWKRWLNYQSIVKQVPFFLFLALLAVLYIY
                     NGHYANKTIRNINRTEKEVKELQYEYTAIKGELISRSKQSELIKAVEPLGLKELIESP
                     VVLSDSSGIKK"
     gene            complement(14735..15649)
                     /gene="rsmH"
                     /locus_tag="PLO70_08315"
     CDS             complement(14735..15649)
                     /gene="rsmH"
                     /locus_tag="PLO70_08315"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQZ74508.1"
                     /translation="MQQQYHIPVLLDEVIEGLNIRPDGTYVDCTFGGGGHSRAILSKL
                     SAKGRLVAFDQDDDARKNLPDDERLIFISHNFRHLQRFLRLQQITTVDSIMADLGVSS
                     HQFDEAERGFSTRFNAELDMRMDKRQTVTAFDVVNTYTEQRLHKLFEQYGEVTNAKTL
                     AKTIVQVRNTASLKTVDGFKNAVREIVKGNPNKYFAQVFQALRIEVNDELGALEEMLQ
                     QVPTLLKPGGRIAIITFHSLEDRLVKNFFRRGSFDEPEENPFINTETVNELKVITKKP
                     VQPSEKEMKQNPRSRSAKLRVAEKIMMV"
     gene            complement(15659..16117)
                     /gene="mraZ"
                     /locus_tag="PLO70_08320"
     CDS             complement(15659..16117)
                     /gene="mraZ"
                     /locus_tag="PLO70_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQZ74509.1"
                     /translation="MTGFLGEFEATLDAKGRFLLPAGFKKQLPEEETVKFVINRGFEK
                     CLALYPMRTWEPLFEKITGLNEFDPKQREFRRAFLNGATYVEPDSAGRILLPPNLKAY
                     AELQKDIVLMATGDKIEIWDSNKYKKIFDTLSSDTFSDLGRDVLGGGKTE"
     gene            16385..17617
                     /locus_tag="PLO70_08325"
     CDS             16385..17617
                     /locus_tag="PLO70_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HQZ74510.1"
                     /translation="MADPQNISIKDYTYALPEERIAKYPLAERDASKLLIYNDGALTE
                     DIYRNITYHIPGHSLLLFNNTKVVEARLLFKKETGAVIEIFCLEPHEKYSDITTAMLQ
                     KTKVEWQCLVGGISKWKKGQVLEKKIKSGNNEIILRANYVEKRTDTFIIELSWSPGEL
                     SFAEVLHTAGAIPLPPYIKRSAEKSDTERYQTVYARYDGSVAAPTAGLHFTDHIFNKL
                     QEKKIKTDFVTLHVGAGTFKPVKAETMQEHEMHAEWIDVSKSTIENILQNLDNNIIAV
                     GTTSLRTIESLYWLGVKKSQIKSQEPGELLQWEAYGLAEKNIPVHDALQYLLEWMIDN
                     KIERLITKTQILIAPGYQFKIIRGLITNFHQPQSTLLLLVAALIGKDWKSVYEYAFHN
                     NFRFLSYGDGSLLWSKRN"
     gene            complement(17614..18951)
                     /locus_tag="PLO70_08330"
     CDS             complement(17614..18951)
                     /locus_tag="PLO70_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQZ74511.1"
                     /translation="MKRIFTIITVLISLSLIGIIVIQVSWIKNMVLLREEQIKHNVEE
                     STKMVGNELSQHKGSYASGLNRRGFLSEELGIDLFKPVTIGQRFDVEEIKKRFNKAFL
                     QNNLKDIQFEFGIASFDRLGNNVFEKISPNFINAYEDTLNNFSFNWGLEALSGTAGEN
                     LGVNEVLFVVIPNIKGLVLKSLRWKIATSILFTLIILAAFYLTVRTVLRQKKLGEIKN
                     DFINNMTHEFKTPIATISLAVDAMRNEKVINDRQKLSYFSGIIKEENQRMNRQVETIL
                     KASQLEKQEVDLNMKPVHVHEVIKDVVDNFTLQLEDKKGRVELSLNAPNDLIEADEVH
                     FSNLVNNLVDNAVKYAKDNTPPVIKLITSSNGKNFTVKIEDNGIGMNRDTVKRIFERF
                     YRAHTGNVHNVKGFGLGLSYVKTVVEAHNGDIKAESTLGKGSSFTIELPLKKA"
     gene            19216..19767
                     /locus_tag="PLO70_08335"
     CDS             19216..19767
                     /locus_tag="PLO70_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQZ74512.1"
                     /translation="MIEPAPGILLIADPFLKDPNFLRTVVFLCEHKEEGSFGFVLNRQ
                     YENTLDELIPELEGHKLPVFYGGPVQIDSLHFLHQYPDLIPGGQEVIKGVYWGGDFDA
                     LVNLVKSGAIDTNKIRFYIGYSGWSEGQLDSEMNEKTWLTVKANRKLVFHKDFTEIWK
                     DSLRHMGGDYEMMVNFPIDPQLN"
     gene            complement(19883..20140)
                     /gene="atpC"
                     /locus_tag="PLO70_08340"
     CDS             complement(19883..20140)
                     /gene="atpC"
                     /locus_tag="PLO70_08340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HQZ74513.1"
                     /translation="MNLEILTPEKKLYSGEVYGVQMPGISGSFEVLDRHAPLVSALKP
                     GRLKVLKDKQNSLVNFDIQGGFVEVLNNKVTVLVEGAVSVE"
     gene            complement(20151..>21119)
                     /gene="atpD"
                     /locus_tag="PLO70_08345"
     CDS             complement(20151..>21119)
                     /gene="atpD"
                     /locus_tag="PLO70_08345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQZ74514.1"
                     /translation="FAGVGERTREGNDLMREMIEAGIMKYGDAFKHSMEEGGWDLSKV
                     DMKELAESKATFVFGQMNEPPGARARVALSGLTIAEYYRDGDGQGKGRDILFFVDNIF
                     RFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKNGSITSVQAVYVPADDL
                     TDPAPATTFAHLDATTVLSRKIADLGIYPAVDPLDSTSRILSPLIVGEAHYECANRVK
                     SMLQRYKELQDIIAILGMDELSDEDKLVVSRARRVQRFLSQPFHVAEAFTGLKGVLVP
                     IEETIRGFNMIMDGEVDEYPEAAFNLVGTIDDAIEKGKKLLAQAQG"
BASE COUNT         5762 a         4914 c         3591 g         6652 t
ORIGIN      
        1 agcagaatcc tcaacctcgg tggacaaagg tgactgagcc tgatgggcca tcggcatatc
       61 cgccgctgga atgtcatccc tctctattat ttgcatttgc aactccagga gttcttcctg
      121 ctcattgctg gcaagtttag aagggaccgg ttcttcggca gaagtctttg aggtaacgac
      181 ctctggtttt gattctgcgt agatattgga cggtctggcc agataacctc ccgatgctgc
      241 agacaccggt gttgtactac tagtactatc tttcatactg aggatcagct cagcatctgt
      301 ttcttttata gtggggggaa cggattctga ttgtgaaacc gaatgtatac tttcatcttt
      361 tgctacagtt gacattttgc tcacagtaga tatactaatc atttcatccg taatcttact
      421 aagtaatttt tcttcggatt gcagttccca gtgtataaca acaggttctt cttccttaat
      481 gtcgctcaga tcaataaccg gagcagcaac aggaaactct tccactaatt tgggcattaa
      541 ataatcctct ttctttgtta cctctaatga actttttgtt gcttccattt ccttttcaag
      601 caacgcctgc tgtatttcta ctttttgatt gataggaatc accggcgttt cctctgtttg
      661 tcccagaatc ataacaatct tttcctcagg tttcggctct tcctttttgg gtattggttt
      721 ggcaaacgga tccttatgct caaagccggt agcgataagt gtaataccta tcttatcacc
      781 taaagtgctg tcataaccca taccaagaat tacatctgta tccacacctg cctgcgtcaa
      841 caggtaattt tgtattatct ccacttcatc catggtaaac tcatgttcac cctgggcaga
      901 gttaatgttt atcaatatcc actttgcacc ctggatatcg ttatcattca gcaatggtga
      961 attcaatgcc tcttcaatag ctttctgtgc cctgttctca ccagcagctt ctgcattacc
     1021 aagaatagct actccaccgt tgcgcattac agtacacaca tcagcaaagt caacatttat
     1081 ctgaccggta ctgttgataa catcagtgat acatttcgca gctgttgcca gtacattgtc
     1141 agctttttca aatgcttccc gcattttcag atttccaaac tggtgacgca gtttatcatt
     1201 actgatcact aataatgtat cgacatatcc tttcagtgtt ttgatacctt cttcagcctg
     1261 caactgtctt ttctttcctt cgtaagcaaa aggtgtggta actataccaa cggtaaggat
     1321 gccaaggtct ttacatattt tagcaatgat aggtgcacca cctgtacctg tgccaccgcc
     1381 cataccggca gtgatgaagg ccattttggt gtttacttcc agtatccttt ttatttcttc
     1441 taatgattct tcagttgcct gtctcccgat gtcaggatta gcaccggcac ccaaaccctg
     1501 agtaagatgc ggtccaagtt gtatacggtt aggtatgcca ctgttggata gtgcctgagc
     1561 atctgtattg catataataa agtttacacc gtcaatctgc tggttgaaca tgaagttcac
     1621 tgcattgcct ccgccaccac ctacaccaat cactttcaga atggatgatt tttctttagg
     1681 cagatcaaaa tgtatcatac gttttggatt taattggctc ttttaaagcc gttgataatt
     1741 tgagttaata attttctttt caggtatgag attataaatg cttgtcttct tcttctttga
     1801 acaagtcgat gataccatct ttgaatttgc cccagaagtt gtttagtcct ttccgctgtt
     1861 tcactttgtc atcgctcact acttcttcca caacaggttc gccctcttct tcagcgactc
     1921 ttctcaatcc atcaggcact tccactttct tatattcctt ttcaaactgt ttcctgttat
     1981 gttcataatc atcataccct ttcaggataa gaccaataca ggtagaatat gttggtttag
     2041 acagttcttc gatatgcccg gcagcgagat gttcgttagg gaaaccgatt ctggcattta
     2101 acccggttac atattcagtc agctggatca ggtgtttcag ttgtgatccg ccaccggtga
     2161 gtatgatgcc gccattcaat gctttattat ctaagccaac ttgttttaaa tgatacgtta
     2221 caaaatccat gatctcactc atacgggcct ggataatgtt agccaggttt tttacactga
     2281 tttctttggc aggcatgcct cttaaacccg gaatagtgat ataggcattt gcttttgctt
     2341 cattagccaa agcgctgccg aactgtactt tcatctgttc agcctgcgtt ttcaatacac
     2401 ccagaccggt tttgatatca ttggtaatgt tttctccggc aaaaggaata acagcagtgt
     2461 gttttaaaat cccctcgtaa aaaacagcca ggtcagtagt accaccgcct atatccacaa
     2521 tagctacacc agcttccagg tcttcctggc ccattaccgc agaggaagaa gctaatggtt
     2581 gtaatacgag gtctttggta tgtaaacctg acttctccac acttcggttg atattacgga
     2641 ttgcattctt atcaccggtg atgatatgaa agttggcacc gatctttacc ccaccatacc
     2701 caatcgggtt agggatgttc tggaaattat caacggtgaa ctcctgtgga ataacatcaa
     2761 tgatctgatc gccggcaggg atatacgttt tatattgatc atctatgagc tgatcaatct
     2821 cccgttgtgt aatctcctcc tctgttttct gccgcacaat atcacctcgg gtttgcagac
     2881 tcttaatatg gtggcccgcg attcctacat atacttcatt tatatccagg ttaggattgg
     2941 aagcaaaaca gttttctaat gcagttttga tagctttgat cgtttcatca atattcagga
     3001 cctggccatg cttgacaccg ttggaattag acttaccaaa tccaaggatt tccagtttgc
     3061 caaactcatt cttacgtcct gcaattactg caatcttggt tgttccaatg tcaagaccga
     3121 caataatggg ttgctcgttg ttcataaatg ttgtttttag ttgttgttgt ttttatcgaa
     3181 atcgttaata tagttttatt gatacccccc attgggatct tctgtcactt tcggaggcat
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgtcatttt ctgcatccct
     3361 tgactgctgc aataattttt caacattttt tttcaactgt attgagtcta ctttggaatt
     3421 accggcatat cttgacgcaa cgacctgtcc tgcataccgt acatctatta ctttgtattt
     3481 atcaaatcct gttttactaa gcacctgctt gtaaaatacc atcagccggt tgaactgtct
     3541 ttcaatattc tccccttcac ccatcttcac aatgtgatta cctactaccg gtatcatttc
     3601 aaaagtccgt tccggtgtga tatcaatttg tgccacctgt gccaaccaaa aggaatcatt
     3661 aactatataa ttagccatta ctgttaattt tttcagtaac atgctatcaa ttgccgtaat
     3721 aattttctta gccggaaatc ctgtaaacac aggcaccctt gcacttagtt tctcagataa
     3781 cggcatcctc atacccgatt catcaatgta gaatgaatat ccttctgtat taaatatcct
     3841 ggcaaccgga tctttttctg tgacagagat atgcagcacc tcctggttat caatataaag
     3901 ttccgcatca cttatccatg tattattttc cagcattttt tccagttcat gcaggttcaa
     3961 ggatgataaa ggttgtccct taatacttcc ttttgatgct ttctttaatt gctgttcgac
     4021 atcgttttta tcaataaaat aattgttcag agaccctttg attacaatgc tgtaatcttt
     4081 acaaagacct ttatttctct tactgatagc ggccagcaac agcgtaaaca tacctccgcc
     4141 aatacacagc cagactgtga caaataaaat tttccttatg gttgattttg cattcactct
     4201 ttataaatct tttaattcat ttttcatcgg ttccaccagc atatcaatat ctcctgcccc
     4261 tgctgttatc aatacttcac caaactcctt atttaaggtc cttgcataat cctgctgaat
     4321 ccaacttatc aactcttctt ttttaagcac tcttttatgc tcattctcca ttttgtccag
     4381 caccatttca ctgctaaccc cttctatcgg taactcccgg gctggataga ccggcaagag
     4441 aatcacttca tcagccaaat caagtacctg cgcaaatccc tcagccaggt ctctagtcct
     4501 tgagtacaag tgtggctgaa atatcactgt acattttttt tgtttaaaca aggttttggc
     4561 gccgtttatc aatgctctta attcttccgg gtgatgagca taatcatcaa taaatactaa
     4621 tcgttcattc ctgatgacat actcaaatct tctcttcaca ccccggaaat tttcgatcgc
     4681 cgcttttatc ttatcatttt ctatccccaa agaactagct actgtaatag ctgcaacggc
     4741 attttctaca ttatgcatcc ctcccatatt cagccgcaca ttctccagct tattatcttt
     4801 catcattaca tcaaactcat aactgccatt catcatccta atattagcag catacacatc
     4861 agcactttca ttttgcaagc tgtatgttaa atgccggtct gcattcaact ctttcccccg
     4921 ctttaaacca aattgcctta ccagtaaccc ctccagcttt actcttttac tgaaatcaat
     4981 aaaagcctct tccatcgcct ctgccgttcc gtaaatatcc aggtggtcag catccatcgc
     5041 tgtgatgata gccacatccg gacttaattt caaaaagctt ctgtcatatt catctgcttc
     5101 aatcacacaa acatttcttt cactgctcca gaaatttgtt ccataattca ctgcaattcc
     5161 acccagaaaa gcattgcaac caaaaccact gtcacggagc aaatgcgcta ccattgttgt
     5221 aatagttgtt tttccgtgtg tccctgcaat acatatatta aaagaacttt cacttattat
     5281 ctgtaataca tcacttctct tcaccacttt atatccattg gtttgataat aaaccaattc
     5341 cttatgctct gcaggtatcg ccggtgtgta aactaccagg tcaacatttt ttggaaccag
     5401 ttctacattc tcttcataat gaaccttaat gccgctggct tccagttctt ttgtcagtac
     5461 ggttggtgtt ttatcgtatc cgcttacttt cacgccacca gcatgaaaaa accgtgcaat
     5521 ggcactcatc ccgatcccac cgatgccgat aaaatacact tcattcatct tttcaatccc
     5581 ccgaaagggg gtattcataa ggacagtatt ggcttgtacg ttcatgtgat taaatcactt
     5641 tcaatatttc tgctgcaatc actttatcgg catcattaat ggcttgtgca gcaatatttt
     5701 ttttcaactc ctcctgttta ctctcatcca gtgacagttc aattgtcata aacaccagtt
     5761 tttgccgggc ttcactgtct tttaccatca atgctgcttt cttgtttacc agttgcatcg
     5821 cattaaccgt ttgatgatct tctgcagcaa aaggataggg tacgaacaaa acgggctttt
     5881 tagcgacaca taattcagca acggtcatag ctcccgaacg ggccactaca atatcagcag
     5941 cagcatacgc atattccatc tgcgttataa actcattgac ccacactgac tgttttccat
     6001 aggctctctc ttttgcttta accgaataag gtttgcctgt ttgccagata acctgtaatc
     6061 cggctttcaa catttcatcc agtcctttat ctattgcttc gttgatggat ttagccccaa
     6121 ggctaccacc aacaaccagt actgttttct ttttttcatc cagtgaaaag aatttaattc
     6181 cttcactcct ggttactgat gcacttgcaa taaccactct caccgggttt ccggttactt
     6241 ttactttcct ggcaggaaag aatttttcca tcccatccgt accagtaaaa attgcagtcg
     6301 cctttttacc cagcatgata ttggacttgc ctgcaaatga attggactca tgaataaaag
     6361 tttttactcc ttttgcctgt gcaaaccgca acaccggaaa gctggaatat ccacctactc
     6421 ctattgccgc atccggatta aactttttaa aaattgtccg gacctgtaaa aagcttttca
     6481 ataatttata gggtaacccg atatttttta tcaaagagct tctgttgaat ccagctatat
     6541 ccaatccttc aattctatat cctgcctggg gcactttctc catttccatt cttccttttg
     6601 cccccacaaa taatatttct atacttccgt ctattttctt cagtgcattg gcaattgcta
     6661 ttgctggaaa aatatgtccc cctgtaccac cacctgctat aattactctt ttgctcattt
     6721 caccaatact attttgcaaa atcagctgct gcttttcctt ccagttgctc tacatttctc
     6781 gccacactta aaatgattcc aatagccagg caggtaaata tgaaactggt accacccatg
     6841 ctcaccaacg gaagagttac accggtcaca ggaaacaaat tcaccgtcac ggccatattg
     6901 gctactgcct gtattgccaa tgtgaaactc aaccccaaag ccaggaatgc accgaaggca
     6961 aatgggcatc gcttaaaaat cctgatgcac ctgtataaga acaccaggta aataaacatg
     7021 ataaaagccc cgcccagcaa accatactct tcaataatga tggcataaat aaaatcgtta
     7081 tatgcctgtg gtaaaaaatc acgggttgta ctattacccg gccccaaacc aaatacgcca
     7141 cctttggcaa ttgcaatctt tgcctggttt acctggtagg ctccattatc aacatcatcc
     7201 ttacttccgt aaatgaaact ttcgacacgg ccaatccagg tatcaaccct ggctgtaagt
     7261 gctgaagaag actttttagc aactaccatt tcctctttat ctgatttatg ccgaataaca
     7321 gcagcagcaa ccaggaatgc aattggtatt agtgcaatac caattgtcat caaaatatgc
     7381 ttcgtattca ccctccctat aaataataac atcaaacaac ttgctccaag caataatgct
     7441 gtagaaagat tagccggagc aatcagggca caggtcaggg ccactggcaa gataacaggt
     7501 aaaaagcctt tctttaaatc cttaataaca tcctgttttt tactaagcaa acgggccagg
     7561 tacatgaaca atgccaattt tgcaaggtct gatgtctgca ttgtcatatt tataatcggg
     7621 agacgtatcc agcggctgcc ctcattcatt tttacaccaa aaaataacgt atagaataaa
     7681 agtggaagag aaaggaggaa tattattttg gctgctcttg aataaaaagt ataattaaca
     7741 agatgggcaa aataaatgac catgatacct gccaggataa aagctacctg cttgaacagg
     7801 tatatctctg tattgccttt ataattctta tacgccaatg aaccggtagc actgtacacc
     7861 gccagcaatg aaaccaatgt tagtaatact accaatgccc agataacttt atcgcctttt
     7921 gtcttactca gtaagttatt ggtgttgaaa gtatttttca attcattaaa ccgtatatct
     7981 cttgttgctt ccattatttt tataactctc ttactgcttg cttaaactgg tttcccctgt
     8041 cttcataatt tttgaacaaa tcaaaactgg cacaggcagg gcttaataac acaacgtcac
     8101 cttttgcagc aaaatgaaag gcagcatgta cagcttccaa tgcattgccg gtattcacaa
     8161 taggatttac gatagtagaa aacgcttcat gaattttccg gttatctgcc ccgaggcaaa
     8221 taattgcttt aactttctct ttcaccaggt cttcaatcag tgcataatca ttccctttat
     8281 ccacccctcc caggattaat acggttggct ttgtcatact ctctaatgca taccaggtag
     8341 agtttacatt ggtggcttta ctgtcattta taaactcaac cccacgaacc gttgctacat
     8401 gctccatccg gtgttcaagg ttttgaaaat tctgcactgc atcacggatc ttgtctttcc
     8461 gtatatccag tgtagttgct gctacacaag ctgccatcgt gttatactgg ttatgcttcc
     8521 cttttaatgc aaaatcaaat acactcattg tagtaaaatc ttgtcctgtt cgtacataca
     8581 tatccccgtc tttaataaat gctccgtttg gtagttccct tttcatacta attggcagtt
     8641 ggtttgattg aatattgaac tggtttagat atttcatagt tacttcatca tcggcgcagt
     8701 aaatgaaata atcattatgc tgctggttca tagtgatccg gaatttgctg cgtatataat
     8761 tcccgaagtt gtaatcgtag cgatcaagat ggtcttctgt aatattggtt agtatagcta
     8821 catctggtct gaaactttcg ataccatcca gttgaaaact gctcacctct actacatata
     8881 agggtttggg atcttcggct acctgcttgg caaaagaatc accgatattt cccaccaatg
     8941 cgcaatcaag accagcagtt ttgcaaatat ggtaagtcaa tgccgttgtg gtagtcttac
     9001 cattgctccc ggtaattgca atgatcttgc tatctccttt aaaccggtaa gccagttcta
     9061 tttcactgat gatggtaatt ccttttgccc tgattttctt tacgatctcc gccttttccg
     9121 gaatacccgg gcttttcatt acctctcctg cattcagaat cctgtcatca gtgtgccggc
     9181 cttcttcaaa ttcaattcct gcactcttta attcgctacg ataatcatcc ttcaaagaac
     9241 ttccgtctga aaggaatact tcatacccgt ttttcttcgc cagtagtgcg gcacccacac
     9301 cactttcacc tccacccagt atgacaaacc ttttgctcat gttctgattt gtgtacggaa
     9361 cacgattctt cataagtatt ggttttttaa tacaactgca ccacaattcg attggatcag
     9421 aacgttagtc ttcttaagga ttggatcatg ggtttttcgg taatatcctg actgaatcag
     9481 ttgaaccaat caacaaaccc agtttgtatt ctatcttaac ttcagcgtca cgatactcaa
     9541 cacaaccagc aatactgtaa caatccaaaa tctcgttaca atctttacct catgatattt
     9601 tttcacctgg taatgatgat gcagtggcgc tttcaaaaaa atccttcttc cttctccaaa
     9661 ttttctcttc gtatatttaa aatatcctac ttgtaacatc acacttagtg cttctactaa
     9721 aaacactccg cagaaaatgg gtatcagtaa ttctttatga acaataatcg ccagtgaagc
     9781 aataattcca cccagcgtca ggcttccagt atcccccata aacatctggg ccgggtaaga
     9841 attgtaccac aaaaaaccaa cgcaacctcc aatcattgcc gcaataaaaa tggacagctc
     9901 tcctaagttg ggtatgtaca ttatgttcag gtaatctgcg aaaaccaggt taccgctcgc
     9961 ataagcaaaa atgcccagta gtgtaccaat caacgctgat gttccggtgg ccagtccatc
    10021 taatccatcg gtcaggtttg caccatttga caccgccgta atgatgaata tcactatcaa
    10081 tatgtaaaga acccatgtat aatccctcca cgaagccggc aataattttt cataattgaa
    10141 ttcatggttt ttaacaaatg ggatcgttgt tataaccgat tttgttggaa caaaatattt
    10201 tatgctgcca tttacattct ttgcaataac attagtcgta tcagcaggag gtactttacc
    10261 tacatactct ctccaaattt taacctcgct attgaaatag agcgtagctc caaccaaaat
    10321 acccagaccc acctgtccca gaattttaaa ccagccagcc aaaccatctt taccagactt
    10381 cttataggtt tcaccttttt gttgtgccag ccgcttgcct ctcagtttta aataatcatc
    10441 aataaaacct atgattccca gccatacagt acttatcagc aatacctgca catatacatt
    10501 cgtcaaatca gccagtaata atgtggggat aattattgcc aggataataa tgatacctcc
    10561 cattgtcgga gtacctcttt tactctcctc acctgctaat ccttcctccc ggatactttc
    10621 actgatttgt tttttctgta aaaattttat cagctttttc ccatacacca gcgttataaa
    10681 aagcgacaac aataccgcag ccagtaccct gaaagtgatg aacctgaaaa gttcacttcc
    10741 gggaaacctt attccttctt ctttcagcca atcaaataaa tgatacagca tgctggttat
    10801 tgatttactt gcctaataat tcaaaaagtt ctcttactac ttctttatcg tcaaagtgat
    10861 tcctcactcc cttaatctcc tggtatttct catgcccctt acctgctatc aacactatat
    10921 cttccgcttt tgccaaactg attgctgttt ttattgcctc ttttctatcc actatcgaga
    10981 tatattttct tttataggct actgctaatc cttcctccat gtcttttatt atctgtgccg
    11041 ggtcttcgcc tcttggatta tcactggtga aaatcacttt atcgctatgc tcgcaggcca
    11101 ctgtcgccat caccggtctt tttgttctgt ccctgtctcc accacaacca acaactgtaa
    11161 tcacctgctc aaaacctttt ttaagtttct ttatggttgc cagcacattc agcaaagcat
    11221 caggagtgtg agcataatcg actatcgcaa ttactttttc ctttgctgac acctggtaat
    11281 caaaccttcc ttcagcacct gtcagctcac tcaatgccgt caatacttcc tgtttatctt
    11341 ctcctaaaca aatggctgca ccatatactg ccagcaggtt gtaagcattg aactccccga
    11401 tcagcctgaa atgcacttcc acctcgttta cgatcatcat caaaccactc aggctattat
    11461 ccaaaatctt tcctttgaaa tcagctactg ttttcaaact gtagtaatac tttttcgcat
    11521 tggtattctg cagcattacc attcctcttt tatcatccgc attactgatc gcaaatccag
    11581 atgaaggcaa cgaatcgaaa aaagtcttct tcacccggat atactcatca aatgttttat
    11641 ggtagtcaag atgatcatgt gtgatattgc tgaaaatgcc gcctgcatac agtaaacctg
    11701 ttacccggtg ctggtgcaaa gcatgtgaac ttgtttccat gaacacatga ctacaacctt
    11761 catctgccat ttgctttagc aatgcgttga ggctgatggc atccggagtc gtatgcgttg
    11821 caggcaccac tttgtcacca atctgattct ccacagtgct taacaatcca catnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactgacc tgattgacac tttatatagt
    12001 acatcttgta ataacacgaa tgatctttaa cttttttact ttctatcaac ttaactcaag
    12061 tactacagta acaccattta ccaatgaggt tcccggcgct atcgactgtg ttgcgatttt
    12121 cccttttcct tttattgtca ctttcactcc catgttttcc agcaggtata gagcgtcctt
    12181 caaacccatt cctcttacat ttggcattac ctgtttacgg acagaatttg cttttaaaac
    12241 cggctgataa ttacttgcat aaacattact ccaattgttt tgtgctacag aatctgtaaa
    12301 gcggacattt aatgctttat aaactttctt gatatcagtt gaatatccgg catagaaata
    12361 agcagcacta tcttttacag ttgtaaacaa tgaaggatct ttcctgtcaa catacatagc
    12421 atataacttt gtagctattt cccgaaaaac cggagctgca acggtgcctc cataatggga
    12481 agctgcatga ggttttgtac gaaccactac gatacagcta tactgtggtt tatcggcagg
    12541 aaaataacca acgaatgagg cctggtaaac accatgcccg tattttattg cgccgtctga
    12601 aacttgtgct gttcctgttt tcccggcaat gggaaatggt actcctgcaa aaactcttct
    12661 ggcagttcct tcagttacta cagcttccat agaagctctt gcagcctgga taactgcggg
    12721 tttgcaaata tttgcctcta ttatggttgg ttcaaattgt ttgtgaagta tcccgccact
    12781 ttgtacatga cttacaagat agggtttcat cattttgcca ttgttggcaa cagcgttgta
    12841 taatgtcaac gtatgaaggg ggctaacctg taaagcataa ccgaaactca tccataacat
    12901 attacccagt gagctacctt tttcattcaa cggagccatg gttggtttcg gaacatccgt
    12961 aagatcgatg ggcgaacgaa catccaggtg aaagcgatga agaaattcct taaactcctg
    13021 tggcttatta ccaaaagctt tataggctaa tttgctcata ccaacgtttg agctgtgggc
    13081 aaaacattct ttcacactca ataatggttt aggagaacgt tctgcatccg ttacaatctt
    13141 agggcctacc tgcaaacgac cagcacttcc tacttctacc atatcatcaa tagcactgct
    13201 tcctttttcc aatactgata aaagtgttac cagcttgatg gtagaaccgg gttccgttgc
    13261 ccgtaaagca taattatcat cttcccaata acttccatcc ggtctgcggc caagatttgc
    13321 gattgctttt actttaccgg tacttgtttc cattacaata gcagtaccgt ataaagcctc
    13381 actttgcagc atcattctca tcaatgctgt ttcagtgata tcctgaattt taacatccag
    13441 tgtggtaaaa atatctttac cgttttcggg atctgtttct gaaccttcaa taggaatcgc
    13501 agtaccgcca gctatatagc gtaccagttt ttgtcctttt ttaccactta atatggtatc
    13561 atagcttctc tccaatccta cattctgctt tttgatcttt ccatcactgg ccatatgctc
    13621 ccgggataaa ccaatagtcc tgctggccaa taaaccaaaa ggaggtaatc gcttactatt
    13681 cgtttcaaca atgatcccac tcttatttct accaagtctt gccaatggga actccctgaa
    13741 gattttgtat tgctcaaaac tgagtttctt tttcagctga taatatcttg tattcttttt
    13801 gtaggcaagc tttaattcgc ttttatactg agctgctgtt ttatcaccaa agtaattagc
    13861 taaagcaata gaaaaggagt ctatattttc agtaaatcgt ttgcctttct tttctctcag
    13921 gccatcggca ttaaaatcta tataaacatc aaaggtggga atagaagtac tgagcatctg
    13981 tccgtcttcg ctatagatag ttccccgttg tgcatcgagg gcgataaacc gctgatgaag
    14041 gctgtcactc atactgcgcc agtgattccc ttcaacacgc tgaatgtaga aggctcttcc
    14101 gagtaccagt aaactcagca ccacgatgcc aaggaaacta acatatactc tccacagtat
    14161 gtcacgtttt acttccaatg ccgctttgct tgtgttcgtt attagttgtt cgatgctata
    14221 tataactgct tcctgcagtc cgtccgttgt tcgctatttc tttataccac tgctatccga
    14281 taaaaccaca ggcgattcaa tcaactcttt caatcccagc ggctccacgg cttttatcaa
    14341 ctcactttgc ttactccggg aaatcagttc cccttttatg gccgtgtatt cgtattgcaa
    14401 ttcttttact tccttttctg ttcggttgat attcctgatg gttttatttg cataatgtcc
    14461 attatagata tacaacactg ccagcaatgc caggaacaaa aagaacggaa cctgcttaac
    14521 aatagactga taattcagcc atcgcttcca gtcaagcctg ggatcccgtt cttttggatt
    14581 ttgctgttcc tgcataaatg agtttttaac tcgttagttt ggtttttcat cccccgactt
    14641 acatcgaaga cagggttgat ttcaaaggat atagattttc cagattttta taccctttta
    14701 tagtgtacgt aataaaagct tagtcatatc cccatcacac catcattatc ttctcggcca
    14761 ctctcagttt tgcacttctt gatctggggt tttgtttcat ttctttctcc gaaggctgta
    14821 ccggcttctt tgttatcact ttcagttcat ttaccgtttc cgtattgata aaaggattct
    14881 cttccggctc atcaaatgaa ccacggcgaa aaaaattctt taccagtcta tcctcaaggg
    14941 aatgaaaagt gataatcgct atccggcctc cgggctttaa cagggtgggg acttgttgca
    15001 gcatttcttc taacgcacct aattcatcat ttacctcaat tcttaatgcc tggaaaacct
    15061 gggcgaagta cttattggga tttcctttta ctatttcccg gactgcattc ttaaatccat
    15121 caaccgtttt aagcgatgcg gtgttcctga cctgaacaat tgttttggct aatgttttgg
    15181 cattggtaac ttctccatac tgctcaaata atttatgcaa tcgctgttca gtataagtgt
    15241 ttaccacatc gaaggctgta acggtttgcc gcttatccat ccgcatgtcc agctcggcat
    15301 tgaagcgggt agagaagccc ctttccgctt catcaaactg gtgactgctg accccaaggt
    15361 cggccatgat gctgtctaca gttgtgattt gctgtaaacg aaggaaacgc tgcaggtgac
    15421 gaaaattgtg tgaaataaaa attaatcttt catcatcagg cagattcttc ctggcgtcat
    15481 catcctggtc aaaagcaacc aaccttccct ttgcacttaa ctttgaaaga atggcccttg
    15541 aatgaccacc tccgccaaaa gtgcaatcga cataagtccc atcgggccgg atgtttaacc
    15601 cttcaatcac ttcatcgagc agaaccggta tgtgatactg ttgctgcaca attagtaatt
    15661 actcggtttt acctccaccc aaaacatctc ttcccaaatc gctgaatgtg tccgaagaaa
    15721 gtgtatcaaa gatctttttg tacttattac tatcccagat ttctatttta tctcctgtcg
    15781 ccatcagcac aatatccttt tgcaactcgg cataagcctt caggtttggc ggcaacagta
    15841 tccggccggc actgtccggc tccacataag tggcgccatt caggaatgcc cgtctgaact
    15901 cccgttgttt ggggtcaaat tcattcaggc cggtaatctt ttcaaagagt ggctcccatg
    15961 tccgcatggg ataaagagcc aggcattttt caaatcccct gttgataaca aactttaccg
    16021 tttcctcttc cggcaattgc tttttgaacc ccgccgggag gaggaaccgc cccttggcgt
    16081 ccaaagtagc ctcgaattct ccgagaaagc ctgtcattat cagcgatttg agtcaatttt
    16141 gaattaattg acacaaaata acactttttc ccacttaaca accaaatttt tcaatattta
    16201 atttttcccc accttatttt ttgctaaaag tcaatgtggc aaaggatttc atagaatgtt
    16261 ggtcaattag tagaaaaaaa caggtgaata gactgtgaat agcagtggat aacatgttga
    16321 ttacctaaat aaggccggta attcagatat ttgcctctta tcaaatgcaa cagttggttg
    16381 tgttatggct gatccgcaaa acatatcgat taaggattat acttatgctc ttcctgagga
    16441 aaggattgct aaatatccat tagcggaaag ggatgcttcc aaactactta tatacaatga
    16501 cggtgcatta acagaagata tttatcgaaa tatcacttac catattcctg ggcattcatt
    16561 gcttcttttt aataatacca aagtagttga agcaagactt ctgtttaaaa aagaaacagg
    16621 agcagtgatt gaaatttttt gcctggaacc acatgaaaag tacagtgaca taaccactgc
    16681 gatgttacaa aaaacaaaag tagaatggca gtgcctggta ggcggaatat ctaaatggaa
    16741 aaaggggcaa gtactggaaa agaaaataaa atccggcaac aatgaaatta ttctcagagc
    16801 aaactatgtt gaaaagagaa cagatacctt tatcatcgaa ttatcatggt cacccggaga
    16861 attaagtttt gctgaagttc tccatactgc aggagcaatt ccccttccac cctatattaa
    16921 aagaagtgct gagaaatcgg atactgaaag ataccagact gtttatgcac ggtatgatgg
    16981 ttccgtagca gctcctacag ccggtcttca ttttacagat catattttta acaaattgca
    17041 ggagaagaaa attaaaacag attttgtaac actccatgtg ggtgccggaa cctttaaacc
    17101 cgtgaaagct gaaaccatgc aggaacatga aatgcatgca gagtggattg atgtatccaa
    17161 atcaacaatt gagaatattt tacagaacct ggataacaat attattgcag taggtaccac
    17221 ctccctcaga actattgaaa gcttgtattg gctgggagtg aagaagtcac aaatcaaaag
    17281 ccaggaacca ggagaactgc tgcaatggga agcttatggg ttggcagaaa aaaatattcc
    17341 ggtacatgat gctttgcaat acctgcttga gtggatgatt gataataaaa ttgaaaggct
    17401 gatcacaaaa acacaaattt tgatagcacc cggatatcag ttcaaaatta taagaggatt
    17461 aattaccaat tttcatcagc cacaatccac attattgctt ctcgttgcag cattaattgg
    17521 taaagactgg aaaagtgtat atgaatatgc ttttcataat aatttccggt ttctaagtta
    17581 tggtgacgga agtttgcttt ggtcaaaaag aaactaagct ttttttagtg gcagctcaat
    17641 tgtaaagctg ctcccttttc ctaaagtgct ttcagcctta atatccccat tatgagcctc
    17701 cacaacagtt tttacataac ttaaccccaa tccaaaacct ttcacattat gaacattacc
    17761 ggtgtgagca cggtaaaatc gttcaaatat tctttttact gtatcgcggt tcataccaat
    17821 tccattgtct tcgatcttta ccgtgaaatt tttcccgtta gaacttgtaa taagtttgat
    17881 tacaggtggc gtattatctt tggcatattt caccgcatta tctacaaggt tattcaccag
    17941 gtttgagaaa tgcacttcat ccgcttcaat taaatcgttt ggggcattca gtgacagctc
    18001 tacccttccc tttttatcct ccagctgcag tgtgaaatta tccaccacat cctttatcac
    18061 ttcatgtaca tgaactggtt tcatattaag atccacttcc tgcttttcta attgagaagc
    18121 ttttagtatc gtttccacct ggcggttcat ccgttgattc tcttccttga tgattccact
    18181 gaaataactg agtttttgcc ggtcgtttat aactttctca ttccgcatgg catctacagc
    18241 cagtgaaata gtggcaatag gtgttttgaa ttcatgcgtc atgttattga tgaaatcgtt
    18301 cttgatttct cccagtttct tttgccggag tacggtacga acagtaaggt aaaatgcagc
    18361 cagaatgatc aatgtgaaaa gtatagaagt agcgatcttc caccgcagtg attttaaaac
    18421 aaggcctttg atattgggaa taaccacaaa taaaacttca ttcacaccaa ggttctcacc
    18481 agcagttcca cttagcgctt ccaatcccca attgaatgaa aaattattca gtgtgtcttc
    18541 atatgcatta ataaaattgg gagatatttt ctcgaataca ttattcccaa gcctgtcaaa
    18601 cgaagcaata ccaaattcaa attggatatc ttttaaatta ttctgtaaaa aggctttatt
    18661 aaatcttttt ttaatttctt caacatcaaa ccgttgcccg atggtcaccg gtttaaataa
    18721 atcaataccg agctcttcgg aaagaaatcc ccgccggtta agcccggaag cataactgcc
    18781 cttatgttga gacaattcat ttcctaccat tttggtgctc tcttccacat tatgtttgat
    18841 ctgctcctcc cgcagtaaca ccatgttctt tatccaggat acctgtatga caataatacc
    18901 tatcagggac aggctgatta aaacggtgat gattgtaaat atgcgtttca cttaaatcaa
    18961 aaattgggtc gtaagatact acttcaatac ttaactgctg attgcaataa atttatcagg
    19021 ccaacaacat tcttaaacca attaacctta caaaaataca tttcaaaaac tgccaccaat
    19081 cccctgaaca gggtattacc gctattttaa cggtgtttta caaaaagtta tcccatatgg
    19141 aagctaaata atcattgcga taaacaaatt ttgaacagaa atttaaaaaa atgaattttc
    19201 atcctaaatt gaagtatgat tgaacccgcc cccggtatat tgttgattgc tgacccgttt
    19261 cttaaagacc ctaatttttt aaggactgta gtattcttat gcgagcataa agaggaaggg
    19321 agctttggtt ttgtattgaa ccgccaatat gaaaacacac tggatgagtt aattcccgaa
    19381 ctggaaggtc ataaattgcc cgttttttat ggcggaccgg tacaaattga ctccttacat
    19441 tttttacacc aatatcctga tcttattccc ggtgggcagg aagtgataaa aggagtttat
    19501 tggggtggtg attttgatgc actggtgaac ctggtaaaaa gcggagcgat tgatacaaat
    19561 aaaatccggt tttatattgg ctactcgggt tggagtgagg ggcaattgga tagtgaaatg
    19621 aatgaaaaaa cctggcttac ggtaaaagcc aacagaaaac tggtatttca caaagacttt
    19681 acggaaatat ggaaagactc actccggcat atgggcggtg attatgaaat gatggtaaat
    19741 tttccaattg acccgcaatt gaattaagac cccctggttt gtatttattt ttatacccaa
    19801 atcacatgaa aaaacctccc gtaaatcggg aggtttacaa taagttttca aagtcctgga
    19861 caatccggtt tttcggattt aattattcta cagatactgc gccttccaca agtaccgtta
    19921 ctttattatt cagcacttcc acaaatccgc cttgtatgtc aaagttgacc agggagtttt
    19981 gtttatcctt caacactttt aaacgacccg gttttaaggc acttaccaga ggagcatgcc
    20041 tgtccaatac ttcaaaactt ccgctgatgc ccggcatttg cacaccgtac acttcgccgc
    20101 tgtataattt tttctccggt gttaaaattt ctaagttcat agtttaataa ttaaccctgt
    20161 gcctgcgcta acagtttctt acctttttct attgcatcat caatagtacc tacaaggttg
    20221 aaagcggctt ccggatattc atccacttca ccgtccataa tcatattgaa accgcggatc
    20281 gtttcttcaa tcggcaccag tacacctttc aggccagtga aagcctctgc cacatggaat
    20341 ggctgcgata agaaacgttg tacccttctt gcacggctta ccaccagttt atcctcatca
    20401 ctcaactcat ccataccaag aatggcaatg atgtcctgca actctttata acgctgcaac
    20461 attgatttta cacggttggc acattcataa tgcgcttcac caacgattaa cggagaaaga
    20521 attcttgaag tagaatccaa tgggtcaacc gcaggataaa tacctaagtc ggcaatcttt
    20581 cttgagagca ccgtcgttgc atcgaggtgg gcaaatgtag tcgcgggagc agggtcagtc
    20641 aggtcatccg caggtacgta aaccgcctgc acggaggtaa tagaaccatt ctttgtagaa
    20701 gtgatccgtt cctgcatgat acccatttca gtagcaagtg taggttggta acccactgcc
    20761 gaaggcatac gacccaacag tgctgaaacc tcagaaccag cctgggtgaa acggaagata
    20821 ttatcaacga agaacaggat gtcacggcct ttcccttgtc cgtcaccatc ccgatagtat
    20881 tctgcaatgg ttaaaccaga caaagccacc cttgcccttg cgccgggagg ttcattcatc
    20941 tgcccgaaaa cgaaagttgc ttttgattca gctaattcct tcatgtccac tttgctcaaa
    21001 tcccaaccac cttcttccat actatgtttg aaagcatcac cgtatttcat gatgcctgct
    21061 tcgatcattt cacgcatcag gtcatttcct tcacgggttc tttcccctac accggcaaa
//
LOCUS       DAORTI010000050        21083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2091_length_21083_cov_6.377497, whole genome shotgun sequence.
ACCESSION   DAORTI010000050 DAORTI010000000
VERSION     DAORTI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21083
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_21083_cov_6.377497"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..635)
                     /locus_tag="PLO70_08350"
     CDS             complement(24..635)
                     /locus_tag="PLO70_08350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ74515.1"
                     /translation="MDSRTHWEKIYTTKAESEVSWFQPYPKTSVEFLELFNLPLTANI
                     IDIGGGDSHLVDVLLEKGYQNIWVLDISAHAIERSKKRLGERASQVHWVVSDVTAFDP
                     PVQFDFWHDRAAFHFLTSEENIMKYVQITTRGIRPGGYLVLGTFSEEGPQKCSGLEVK
                     QYSETSMAVRFQVSFDRIRCVYEDHLTPSETVQNFLFCSFKRK"
     gene            complement(675..1871)
                     /locus_tag="PLO70_08355"
     CDS             complement(675..1871)
                     /locus_tag="PLO70_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74516.1"
                     /translation="MIPAIRKAYNEAFTKESYVAFLQELNGVHPGELDFRVAETPIFV
                     PKDFTEKILDACESIVDIIVDPKFKELTKNAIPKNLQVPGDEAHSHFIAFDFGICINE
                     AGEYEPQLIEMQGFPSLFAYEVLLDDVFRNHFTVPENFSCYLNGYTRETYIQLLKEII
                     IGNHNPENVVLLEIFPRKQKTRIDFYCTEEYTGIKMVCLTELIKEGKKLYYMNNGKKT
                     VIKRIYNRVIFDDLQQQTPEVQEKGKIFFEELELEWCPHPNWFYRISKYTLPFIRHPY
                     VPATFFLNEIKKMPADLENYVLKPLFSFAGQGVIIDVTQQDIDNVKDPKNWILQQKVK
                     YADVIETPDIPAKAEIRMFYFWKDGEIRPVATNNLGRLSKGKMIGVRYNKDKEWVGGS
                     FTYFER"
     gene            complement(1935..2780)
                     /locus_tag="PLO70_08360"
     CDS             complement(1935..2780)
                     /locus_tag="PLO70_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HQZ74517.1"
                     /translation="MSWTEKRTVKVAILDLYEGAANQGMRCIRNILIEWAEKNDLLLY
                     AKEYDVRLKNELPDTSYDVYISSGGPGSPLETRYEDWDIAWCRWLDKMERWNNNPSNT
                     QKKYIFFICHSFQLASRFFNVGLVCKRRSTSFGVFPVHMLENGADEPVFDGLKDPFYA
                     VDSRDYQVIQPNHDLLNEMGASLLCIEKSRPHVPYERAVMGLRFNEYMIGTQFHPEAD
                     APGMSLYLQREDKRKTVIENHGEAKLQSMLEHLEDPDKIVWTYSHILPNFLNHSIEKL
                     HGELV"
     gene            complement(3087..4718)
                     /locus_tag="PLO70_08365"
     CDS             complement(3087..4718)
                     /locus_tag="PLO70_08365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQZ74518.1"
                     /translation="MKKYYIAALFIVLLSCNRKEKIDLLVYNATIYTVDSSFSTAEAM
                     AVKDGKIIAVGKTADIENEFDATEKLDAQGKFIYPGFIDAHAHFVGYGMSLQRVDLTG
                     TNSWEECIERVKIFANENKEGWITGRGWDQNDWAIKEFPNNEILDTLFPDRPVLLTRV
                     DGHAAIANQKALDLAGIKAHDTLTGGEIEEMEGTLTGVLIDNAVDLVSSKIPAATDEQ
                     FKKALQAAEQNCFAMGLTTIDDCGLSFPSVDMIKSLQANGDLKMRLYVMLSDEKANYD
                     YAAANGMIKTDRLNVRSFKVYGDGALGSRGACLLQPYSDKIGHYGFLLSQPEHFDSVA
                     NWISNNRWQMCTHAIGDSGNRTILNTYAKYLKGKNDLRWRIEHAQVVDQNDFSLFGAN
                     SIIPSVQPTHATSDMYWAGDRLGGERVKGAYAYKQLLQQNGWLPLGTDFPVEDISTFK
                     TFYAAVVRKDAKGWPANGFQPENALTREEALRGMTIWAAKANFEEKEKGSLEKGKFAD
                     FVILDSDIMKEVQEKLLSIKVLQTFLNGEKVFEKK"
     gene            complement(4728..6377)
                     /locus_tag="PLO70_08370"
     CDS             complement(4728..6377)
                     /locus_tag="PLO70_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQZ74519.1"
                     /translation="MKYLFTIIFCAGFFAAQSQTKADSIQLVRQTVNLYDLDFTDAEA
                     DSMLESITDFYQLYKGLHRTLPANNIPYPFAFKPAPAGMKIATKKEKIAWNIPLRTEL
                     PANKNELAFYSLPQLASLIVNKKISSVDLTKFFIARLKKWDDTLECVITLTEELALKQ
                     ATQADLELKQGIYRGPLHGIPYGLKDLFAVKGYKTTWGSTPYKDQVVDIDSYVYEQLK
                     KAGAVLCAKLSLGALAYNNKWFGGETKNPWNLRQGSSGSSAGSAAATVAGLLPFAIGT
                     ETLGSIVSPSARCGATGLRPTFGTVSRYGAMVLCWSLDKIGPICRSAEDAAIVYAFIH
                     GTDGNDPSAIRHTFNYTGKVDWTKLRIAYPENYFKRLKADAPEWKVLETYRSLGANIK
                     AVNFPDSAMYPVNLVSIILNAESAAAFDELTRTNRDDLVERQDKDFWPNSFRAARTIP
                     AVEYINANRYRYNLCKSMNDFMKKYDVLIVPSFAGRQLSMTNLTGNPVVCMPIGFNQN
                     GSPVSITLVGNLYDEATILAAAKAFQDKTEHHKKHPLLFKD"
     gene            6441..7475
                     /locus_tag="PLO70_08375"
     CDS             6441..7475
                     /locus_tag="PLO70_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HQZ74520.1"
                     /translation="MRILLFIVVISLFIVSCENADKTSTGSSTTVAQYFNYGDSGVQS
                     AGVKMIPIKTAVGDFKVWTKRFGNNPRIKILLLHGGPAMTHEYMECFESFFPKEGFEF
                     YEYDQLGSYYSDQPKDSSLWTIERFVEEVEQVRQAIGADSTNFYVLGNSWGGILAMEY
                     ALKYQQHLKGLLVANMMASAPEYGKYAADVLSKQMDPAVLAEVREIEAKADFANPRYM
                     ELLIPHFYQQHLCRLKEWPDGFNRTMKHANSEIYVMMQGPSEFGIAGRLAQWDIKNRL
                     KEVRVPTLMIGAKHDTMDPKAMEEQSKLVQKGRYLYCPNGSHLAMWDDQQVFMNGVIQ
                     FIKEVGEGKF"
     gene            7514..7939
                     /locus_tag="PLO70_08380"
     CDS             7514..7939
                     /locus_tag="PLO70_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74521.1"
                     /translation="MKRIVTVFMLVSLLAACDNSEKKEPATTTEELQPAGEVKTETTP
                     AADAGPANRLEGSWVIKRAEGNMASMNEGTVYEFKGDKLSLGKDGFNNPGSTEITDKT
                     FSFQAEGNSYKFMYDYHFNGDTLVTSMQNSKQTFYMVKQ"
     gene            complement(8158..8760)
                     /locus_tag="PLO70_08385"
     CDS             complement(8158..8760)
                     /locus_tag="PLO70_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74522.1"
                     /translation="MDTDWNKEEHPNWYRDVSPMRYLILGSFPPHPSKWEYPFFYPNG
                     QNRFWKILADLAEQNLKWTKSDKTKAVQERYEIMKKLEIGVQNLGFEIERMGKSALDT
                     HIRINKYHDIISIIENHPELKKILLPGYSAINSTARSFIRYIQQKGIEASAIDQVKSE
                     TLFKIYFKGRSIDCVILNSTSTASKIKYDFLLEQFRRNLK"
     gene            complement(8860..9969)
                     /locus_tag="PLO70_08390"
     CDS             complement(8860..9969)
                     /locus_tag="PLO70_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HQZ74523.1"
                     /translation="MNLNYKSFTLGVEEEYMVLDPATYELKSHEQKIVQEGQKMIKDK
                     VKAEMHQAVVEVGTDICADIDEAFTDVAALRKTIGGIAHGLGFAMGASGTHPFSHWES
                     QLITDHVRYSEIVNELQEAARSNLIFGLHVHVGMESREMANHIANSTRYFLPHVFALS
                     TNSPFWEGRMTGYKSFRTKVFDKFPRTGIPDAFDSIEAYDNYVKLLIKTNCIDNAKKI
                     WWDLRVHPFFNTVEFRVCDIPLTVDETITLAALFQAICARIYMLRSKNLNFIQYSRAL
                     LNENKWRASRYGVDGYLIDFGKEEEVNTRALIYELLDFLDPVLDHLGSRHRIAHVHKM
                     LENGTGADRQLAVFEKTKSLTEVAKYIQGQYLVGI"
     gene            complement(10086..11039)
                     /locus_tag="PLO70_08395"
     CDS             complement(10086..11039)
                     /locus_tag="PLO70_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74524.1"
                     /translation="MKKIGILHGKERSFPEAFIERVNSKNVPGIMAENVRIDKAMQGE
                     PSGYAVIFDRISQDVPFYRTWLKNAAVTGCAVINNPFWWSADDKYFNNCLMTKVDVPV
                     PKTAIIPSRDLPDDTSNDSFSNLAFPLDWDTIFNYVGFPAYMKPFAGGGWKHVYRLDS
                     AEDFFAKHAETGQLVMLLQEEIVFTEYYRCYCIGGKYVRIMSYEPRNPHHLRYVADFK
                     PSPERLQQMTDIVLRINKYLGYDFNTVELAIRDGVPYAIDFCNPAPDADVHSVGQENF
                     EWVVETSANYAIEKALSQVDGADNLTWGEYVRRGSNKSPLI"
     gene            11225..12043
                     /locus_tag="PLO70_08400"
     CDS             11225..12043
                     /locus_tag="PLO70_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ74525.1"
                     /translation="MHKTAKASIIRTENVFLKSQFLQREVCIDLFVPAAVSTDAPVSL
                     LLINDGQDMEKMNFHKILDYLYAEQAIEPMLFVAIHAGEDRKMEYGTQNHLDYKDRGA
                     KAPLYTSFIFDELLPYIRQKYAIASFKEKAYAGFSLGGLSALDIVWNRPGEFTKVGIF
                     SASLWWRSVDQDDEEYDDDKHRIMHQQIRNGQFAPWLKFFLQCGNLDEIKDRNHNGII
                     DSIEDTLDLVKELENKGYDRENDIYYLELEDGHHDVYTWGRAMPVFLKWGWGLK"
     gene            complement(12138..15473)
                     /locus_tag="PLO70_08405"
     CDS             complement(12138..15473)
                     /locus_tag="PLO70_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQZ74526.1"
                     /translation="MLKRIVLLFVVALVASPLLFAQITTSAINGAVKNNSDEPLVGAT
                     IVATHQPSGTKYSTVSRAGGQFSIQNMRVGGPYLIEISYVGHDVDKYDNIYLQLAESF
                     LLNPLLTKTTTSLENVVITTGRRSSILNASRTGSVTNIGIRQINQLPTISRSINDLTR
                     ATPQSNGASIGGGNYRQNNFTVDGSDFNNSFGIGSNLPAGGAPISLDALEEISVNLTP
                     FDIRQTGFIGSAINAVTRSGTNTFTGSVYTYFRNEKHRGRRVEKTYFTRPAEEFKQYG
                     FRVGGPIIKNKLFFFFNYETEKQPKTIQTRFASSATRPFGSSDNIVRPSADSLNFISQ
                     YLFNNYGYVTGPFDGYTPDIERTKYMLRLDWNISSRHRFNIRYSQVEGGEPNPPSTST
                     SGAGFNYPSGLTRTGTNSLWYKNSNYFQGANLYSIAAELNSSWGRIANTLRGTYTYQN
                     DSRESESQVFPFVDILSTTGVSASSPYTSFGYEPFSFGNLRKVKTYSIIDNLTWTSGK
                     HNWTVGGQFDLSETINGFQRFATSYYVFNTWADFASALNPNPALRVNPRDFAITYSLS
                     KDFAPAFSAFKFAQYSVYGQDEISLSKNFRLTVGLRLDLPTYPDVPQIITHPLVAQRT
                     FAGGEKINTGNLPDKRIMWSPRFGFNWDIYGDRSLQIRGGTGVFTGRVPFVWIVSQSG
                     DNGMLQITQAFNGQSNTPGPFNPDPAAYRPATVPTAGTIVPGSITALSPDFKNPQSWK
                     STLAMDTKLPGGIVATLEALFTKDLNTAFFRNANLISPSALNVSGYPDNRAIYGSTVP
                     TRFINTLNSAGQFVTNGTTAFNSIVLDNGSRGYYFSLTAKFEKPFSKGFYASVAYTKS
                     LAGNLFDGGGDQPLSAWQGTATVNGANTSPMGYADYVLPDRVVGLVSYRKEYFKHLAT
                     TISMIYNGGIAGRFSYVYGADFNRDGVTGNDLIYIPTAAQVQQMQFASQTVNGVVYDQ
                     TAQRNLFESYILQDKYLKAHRGQYAERNGAQIPWLNRVDLKFMQDIFANVGKSKNTIQ
                     FTLDIFNFGNMINPSWGKLKSINASSILVPTNQNSLIPGGSVVPQFRLATAQGDIITR
                     TFRDNVTVASTYSVQFGLRYIFN"
     gene            15656..16861
                     /locus_tag="PLO70_08410"
     CDS             15656..16861
                     /locus_tag="PLO70_08410"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQZ74527.1"
                     /translation="MFDSIESAIADIKLGKLVVVVDDEDRENEGDFVTAARNVTPEVV
                     NFMSKHGRGLICAALPEERCNELGLELMVNNNTALHETAFTVSVDLLGHGCTTGISAQ
                     DRAKTIQSLIDPTITPCDLGRPGHIFPLRAKKGGVLRRAGHTEAAVDLARLAGFEPGG
                     VLVEIMNEDGTMARLPELTAVARKFDFKIISIKDLIEYRVKLDSLIEEVVRVDMPTQF
                     GNFQLVAFKEKNSSNEHLALLKGTWEKDEPVMVRVHSSCFTGDILGSLRCDCGEQLHK
                     AMQMVETEGKGAILYMNQEGRGIGLLNKLKAYRLQEQGMDTVEANLHLGFQMDQRDYG
                     VGAQMLRHLGITKLRLMTNNPKKRVGLIGYGLEIVENIPIEICPNKHNEKYLQTKRDK
                     MGHEILDKE"
     gene            complement(16877..>21083)
                     /locus_tag="PLO70_08415"
     CDS             complement(16877..>21083)
                     /locus_tag="PLO70_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQZ74528.1"
                     /translation="KPVFASVITDGEADTSATWNKAKTVFKIADLKIINGTFSTNKET
                     DRQAYAHFDGQHILFTEINANLNNSTFIGDTVFAKLKLTAKERSGLEVKNLSADMKMT
                     PQGMAFTNLDLSTNRSNLRHYFSMSYDDMSDMGDFIHKVKMAATFDESYIDSDDIAFF
                     APSLKTWKKQITLQGKVRGTVDDLIGREMVVKAGNSTVLNGDITLTGLPDINQTFIDF
                     KANDFRTTYSDAIQIVPGLKKITNPDLRKIQYVNFKGSFTGFISDFVTYGTINTNMGT
                     IKSDLNMKLPKGEDPVYSGSIATDNFRLGEFLGDKNIGIVSLTGTVKGKGFSEKTRNT
                     LVDGTIKFADYNNYRYNNITVKGKLDKKLFDGAATINDENVKVDLNGVIDFNSLTPRF
                     NLLADVTKANFKNLRLTKDSIEFSGKLNFDFTSSTIDNFLGDARITEAEIKKDGIRLP
                     FDSLILSSTYIDSVKKLTAVSNEFTASVTGDFVIKDLPNAFTYFLNKYYPAYIKPPGR
                     FPGNQDLKFDIYTYYVEEYFKMFIPGITGLNNSHFEGNLNLARNELNLKADIPQFHYK
                     QYNFDDVSLIAAGNADSLVLTGKTANIKINDSLNIPITVFKINARNDSSKVSILTGAN
                     QTVEKADLNALVLTYNDGVKIDIDPSSFTVNGKLWTIDETGELIFRKNNPAQGELVLT
                     EGEQKILLKTKPSDIGDWSDLKVALVNVNLNDFAPFFLPRNRLEGLLSGNILVEDPTG
                     HLKITSEDIQTRFLRLDNDSIGELKATLAYDNVSKELKVNGNTLNQENYLGFNLHLFF
                     GDSLKAKNNIIALKAKTFPLKILERFLGTLFSDIQGFLTGDINITGEFDNLGVSGKGK
                     VKDAGLRVNFTQCYYKIQDTDIELTTEKIDLDGIVLTDTVTGNPVYITGGIDHESFKN
                     MFYDLDISTRKPGTRGDATNRPVQILNTTYNDNKQFFGDVKGTAVLQLRGPQSDMVMT
                     INANASEEDSSYITLPSSSSRESGIADFLVERKYGREMTDEDFKSNETSIIYDVEVTA
                     NPMVTVKVVLDELTGDEIKGKGSGTLNIRSGTTEPLSLRGRFDIEEGNYLFTFQSFFK
                     KPFEIRKGVQNYIEWNGDPYNANIKFEATYRAEKVSFAPLASSLNLTSGISNARGDVF
                     VVATLTGKLFTPDINFALDFPSTSVATTDPELALLVQQMQKNTNELNRQVTYLIVFNS
                     FAPSELAGDVSSGGGLGVSTISGILLGVLSDQINKLFGNLLKSDKYKINLNTSIYNRN
                     LVDASKTALNLSSNVNLSIGRSFFNNRFIISTGVGFDAPLSQGSQSNIQQNIQLLPDV
                     TLEWLINPSGTIRATFFYRTNTDYLTSSSNASTARRTGASISYKKDFDKLSDLFRRKK
                     KVQVVPAETELKKEEEIKENDNL"
BASE COUNT         5903 a         4331 c         4430 g         6419 t
ORIGIN      
        1 aagtctttaa tgcttttcat gacttatttt cttttaaaac tacagaataa aaaattttgc
       61 actgtctcag atggagttaa atgatcttca tatacacatc gtatacggtc aaatgaaact
      121 tgaaacctga ctgccatcga ggtttctgag tattgtttta cttccagccc gctgcatttt
      181 tgaggaccct cctctgaaaa tgtaccaagt accaggtagc cacctggtct tatccctctt
      241 gtcgtaattt gtacatattt cataatgttt tcttcagatg tcaggaaatg aaaggctgcc
      301 ctgtcatgcc agaaatcaaa ttgtacaggc gggtcaaacg ctgttacatc cgaaacgacc
      361 caatgtacct ggctggctct ttctcctaat cttttttttg atctttcaat cgcatgggca
      421 gatatatcca gtacccagat attttgatac cctttttcta atagcacatc caccaggtgg
      481 ctgtcacctc caccaatgtc aataatattg gcagttaaag gcaaattaaa taactccagg
      541 aattcaacag atgtttttgg atagggctga aaccagctta cttcagactc agcttttgta
      601 gtataaattt tttcccaatg tgttctactg tccataaacg ttgtgtttgt gacatagcag
      661 ttttgttatt ttaactatct ttcaaaataa gtaaaacttc caccaaccca ttctttgtct
      721 ttattatacc ggacaccaat catctttcct ttacttaatc gtcccaggtt attggtggct
      781 actggtctta tttcaccatc cttccagaaa tagaacatcc gtatttctgc tttggcaggt
      841 atatccggtg tctcgatcac atcagcgtat ttaactttct gttgcaggat ccagttcttc
      901 gggtctttta cattgtcaat atcctgttgt gtaacgtcaa taataacacc ttgtccggca
      961 aaggagaata aaggttttaa tacataattc tccaggtcag cgggcatctt ttttatctcg
     1021 ttcaggaaga atgttgcagg aacatacgga tgacggataa aaggtaaagt gtatttgctg
     1081 atgcggtaaa accagttggg atgcgggcac cattctagct ccagttcttc aaaaaagatt
     1141 ttccctttct cctgtacctc gggtgtttgt tgttgaaggt cgtcaaagat gacacggttg
     1201 tagatccttt taattactgt tttcttccca ttattcatgt aatagagttt tttcccttct
     1261 tttatcagtt ccgtcaggca aaccatcttg ataccggtat attcttcggt gcagtaaaaa
     1321 tcaattcttg ttttttgttt gcggggaaat atttccagca gaacaacatt ttccgggtta
     1381 tgattaccaa taataatttc ttttagcaat tgtatgtatg tctcccttgt gtagccattc
     1441 aggtagcaac taaaattttc cggaactgta aaatgatttc tgaatacatc atccagcaaa
     1501 acttcatagg caaacaggga agggaagccc tgcatctcga tcagttgcgg ttcatattct
     1561 ccggcttcgt ttatacaaat accaaagtca aaagcaataa aatgcgaatg tgcttcatca
     1621 cccgggacct gcaggttttt ggggatggca tttttggtca gttctttgaa cttcgggtca
     1681 acaataatat caacaatact ctcacaggca tccagtattt tctctgtaaa atctttgggt
     1741 acaaaaatgg gggtttctgc taccctgaaa tccaactcgc ctgggtgtac cccatttagt
     1801 tcctgtaaaa atgctacata gctttcttta gtaaaagcct cattgtaagc tttgcgtatg
     1861 gctggtatca tgctgccggt tttaaacgga agttaatgaa tgattgactg gcagcaaaaa
     1921 agagcaggtc ttttctatac gagttcgccg tgcagtttct ctattgaatg attcaaaaaa
     1981 ttaggaagga tatgcgaata ggtccagaca attttatccg ggtcttccag gtgttccagc
     2041 atactttgca gtttggcttc gccgtggttt tcaatcaccg tttttctttt atcttctctt
     2101 tgcaggtaca ggctcatacc gggggcatcc gcttccgggt gaaactgtgt accgatcata
     2161 tattcattaa accgcaatcc cattaccgct ctttcatacg gaacatgcgg cctgcttttt
     2221 tcgatgcaaa gcagggaagc acccatttca ttgagcaagt cgtggttggg ttgaatgacc
     2281 tggtaatccc ggctgtcaac ggcatagaaa ggatctttca aaccatcgaa aacaggttcg
     2341 tctgcaccat tttcaagcat atgtaccggg aagacaccga aggatgtgga tcttctttta
     2401 cagaccagtc ccacattaaa gaaacggctg gcaagttgaa aagagtgaca aataaagaag
     2461 atgtattttt tctgggtgtt ggatggattg ttattccatc tttccatttt atccagccag
     2521 cggcaccagg ctatatccca atcttcgtag cgggtttcca gcgggctgcc ggggccaccg
     2581 ctggagatat aaacatcata ggaagtatca ggcaattcat tctttaagcg aacatcatat
     2641 tctttggcat acaaaagcag gtcatttttt tctgcccatt cgatgagaat gttacggata
     2701 caacgcatgc cctgattggc ggcaccttca tagagatcga ggattgctac ttttacagtc
     2761 cgtttttcgg tccaactcat tctgcaaact tacaaaattt tttatggaca gcaggtgcat
     2821 acgattgcat ggttgttcac aactgtggat ttcatgattc atgttaagtg ttttccccag
     2881 cagatgtggc agaaaagatc tgcgtgaatc agcgttttaa atcagcgata tcagcgggat
     2941 gtattttttt cccgcagatg acgcagatga gttcgcagat ggggcagaat agatctgctt
     3001 gaaccagcgg tttaaatcag cgatatcagc gggatatttt tcccccacag ataatgcagt
     3061 aaagaatttc tctctaaatt ttgtatctat ttcttttcaa acactttttc gccgttaaga
     3121 aatgtttgca gcactttaat tgataataat ttctcctgta cttctttcat gatatctgaa
     3181 tcaagaatta caaaatcggc aaactttcct ttctccaggc ttcctttttc tttttcctca
     3241 aagttggctt tggccgccca gatggtcata ccccgcaatg cttcttccct ggtcaaggca
     3301 ttttctggct ggaatccatt tgccggccag cctttagcat cctttcttac aacggctgcg
     3361 taaaatgttt tgaaggttga aatatcctct accggaaaat cagtacccaa cgggagccag
     3421 ccgttttgct gcaggagctg tttatatgca taagcacctt tcactctttc accccccaaa
     3481 cggtcacctg cccagtacat atcactggtg gcatgtgttg gctgtacgga aggaataatt
     3541 gaattagcac cgaataaact aaaatcattt tgatcgacca cctgtgcatg ttcaattctc
     3601 cagcgtaaat catttttacc ctttaaatat tttgcatacg tattcagtat ggtcctgttg
     3661 ccactgtcgc caatagcatg tgtgcacatt tgccagcggt tattactgat ccagttggct
     3721 actgaatcaa aatgttcagg ctggcttaac aaaaagccat aatgaccaat cttgtcacta
     3781 tagggttgta ataaacaggc tcctcttgaa cccagtgctc catcaccata aaccttaaaa
     3841 cttcttacat tcagccggtc ggtttttatc attccatttg cagcggcgta atcataattt
     3901 gctttctcat cactcagcat tacatacagc cgcatcttaa ggtcaccgtt ggcctgtaat
     3961 gatttgatca tatccacaga gggaaagctg agaccacaat cgtcaatagt ggtaagcccc
     4021 attgcaaaac aattttgctc agcagcctgt aatgctttct taaattgttc gtctgtggcg
     4081 gcaggtattt ttgacgaaac taagtcaact gcattgtcaa ttaatacacc cgtcaatgtt
     4141 ccttccattt cttctatttc accaccggtt aatgtgtcat gggctttaat acctgccagg
     4201 tctaatgctt tttgattggc aatggctgca tgtccgtcta ctcttgtcag caataccggc
     4261 ctgtcgggga ataaagtgtc cagtatttca ttattgggaa actcttttat tgcccagtcg
     4321 ttctggtccc agccacgacc ggtgatccac ccttctttat tctcattggc aaatattttt
     4381 actctttcta tacactcttc ccagctgttg gtgccggtaa ggtcaaccct ttgcagactc
     4441 ataccatagc caacaaaatg tgcatgggca tcaataaaac cggggtagat gaattttccc
     4501 tgtgcatcca gtttttcagt ggcatcaaac tcattttcga tgtctgctgt ttttccaact
     4561 gcaataattt ttccatcttt caccgccatt gcctccgctg tggaaaaaga agaatcaact
     4621 gtataaatag tggcattata caccagcagg tcaatttttt ctttcctgtt gcaggataat
     4681 aatacaatga aaagggcggc tatataatat tttttcatgc tgaataatta atctttaaac
     4741 aacaaaggat gttttttatg atgttccgtt ttatcctgga aggcttttgc ggccgctaag
     4801 atagtggctt catcatataa attaccaacc agggtaatac ttaccggcga accattctga
     4861 ttaaaaccga tcggcataca tactaccggg ttgccggtaa gatttgtcat ggataattgc
     4921 cgcccggcaa agctgggtac aatgagtaca tcatactttt tcataaaatc attcatgctt
     4981 ttacaaagat tataccggta ccggttggca ttgatgtatt caactgccgg gatagtcctg
     5041 gccgccctga agctattggg ccagaaatcc ttatcctgtc tttcaaccag gtcatcacgg
     5101 ttagtccttg tcagttcatc aaatgcagca gcactctctg cattaagtat aatgctgaca
     5161 aggtttacag gatacatggc agagtcagga aaatttactg cttttatatt ggctcccagt
     5221 gaacgatagg tttccagtac tttccattcg ggtgcatctg ctttcaatcg cttgaaatag
     5281 ttttccgggt aagcgatgcg gagctttgtc cagtccactt tacccgtata attaaatgta
     5341 tgcctgatag cactggggtc attgccatct gtgccgtgaa taaatgcgta cacgatggcg
     5401 gcgtcttctg cactccggca aatgggacca attttgtcga ggctccagca aagcaccatc
     5461 gcaccataac ggctgacagt accgaatgtc ggacgtaatc ctgttgcgcc gcaccgggca
     5521 gatggtgata ctatcgaacc cagtgtttca gtgccgatcg caaagggtaa taaacccgct
     5581 actgttgcag cagccgatcc agctgatgaa ccactggagc cctgtctcag gttccagggg
     5641 ttttttgttt caccaccaaa ccatttgtta ttgtaggcta aagctcctaa tgaaagtttg
     5701 gcacacaaaa cagcaccggc ttttttcaat tgttcataca cataggaatc aatatcgact
     5761 acctggtctt tatacggtgt gcttccccag gttgtcttgt atcctttcac agcaaacaaa
     5821 tcttttaacc catacgggat accatgtaag ggtccccggt aaattccctg tttcaattca
     5881 aggtctgctt gtgtagcctg ttttaaagcc agctcttcgg ttaatgtgat tacacattcc
     5941 agggtgtcat cccatttctt taaacgggca ataaaaaatt ttgtcaggtc tactgagctg
     6001 atttttttat tcacgatcag ggaggccaat tgaggcagcg aataaaaagc cagttcattt
     6061 ttattggcag gcagttcagt ccgtaagggg atattccagg caattttttc ttttttggtg
     6121 gctattttca tacctgccgg agccggtttg aaagcaaagg gataagggat attattggca
     6181 ggaagtgtgc gatgcagtcc tttgtatagt tggtaaaaat cagtgatact ctccagcatt
     6241 gagtctgctt cagcatcggt aaaatcaagg tcataaaggt ttactgtttg ccggaccagt
     6301 tgtatagagt cagctttggt ttgtgattgt gcagcaaaga atcctgcgca aaagatgatt
     6361 gtaaataaat acttcatatc gttggttttg caagtgatga aattaagtaa attctgtacc
     6421 ttttgcacaa atcaactgtt atgcggattc tccttttcat tgttgtgatc agcctgttta
     6481 tagtttcttg tgaaaatgct gataaaacca gcacaggaag cagcactacc gtggcacaat
     6541 attttaatta tggcgacagt ggtgttcaat ctgccggggt aaaaatgatt ccaatcaaga
     6601 cagcagtggg tgattttaaa gtttggacca aacgctttgg taataatccc cgtattaaaa
     6661 tactgttgtt gcatggcggt ccggctatga cccatgaata catggaatgt tttgaaagtt
     6721 ttttcccaaa agaaggtttt gagttttatg aatacgacca gttgggttct tattacagcg
     6781 accagccaaa agacagcagc ctgtggacga tagaacgttt cgtggaagaa gtagaacagg
     6841 tgaggcaagc tatcggtgca gactccacga atttttatgt attgggaaat tcatggggtg
     6901 ggatactggc aatggaatat gcattaaaat accagcagca cctgaagggt ttgctggtag
     6961 ccaatatgat ggccagtgca ccggaatatg ggaaatatgc agcggatgtt ttatccaaac
     7021 aaatggaccc ggcagtgctg gctgaggtaa gggaaattga agccaaagct gattttgcaa
     7081 atccccgcta tatggaattg ctgatccccc atttttacca gcaacattta tgcaggctga
     7141 aagaatggcc ggatgggttt aaccggacca tgaaacatgc caacagcgaa atttatgtta
     7201 tgatgcaggg accatcagag tttggtatcg ccggaagatt agcacaatgg gatataaaga
     7261 acaggttgaa agaagttaga gtgcccaccc tgatgatagg agcaaaacat gatacgatgg
     7321 accccaaagc gatggaggaa caaagtaaac tggtgcaaaa aggccgttac ctgtattgcc
     7381 ccaacggcag ccacctggct atgtgggatg atcagcaggt gtttatgaac ggggtcatcc
     7441 aatttattaa agaggtgggt gaaggaaaat tctgatttaa aattttatct tcccattacc
     7501 taaaccaaca gatatgaaaa gaatagttac agtttttatg ttagtttcat tattggcagc
     7561 ctgtgataat agtgaaaaaa aggagccggc aacaacaacc gaagaactac aacccgccgg
     7621 tgaagtaaaa acggaaacaa ctccggcagc tgatgccggt cccgcaaaca ggttagaagg
     7681 aagctgggtg atcaaaaggg cggaaggtaa tatggccagt atgaacgagg gtacggtgta
     7741 tgaatttaaa ggcgataaac tgagtttggg aaaagacggg tttaacaatc ccggttctac
     7801 ggagatcacg gataaaacat tcagtttcca ggcagaggga aactcttata agtttatgta
     7861 cgactaccat tttaacggag ataccttagt gacttccatg cagaacagca aacaaacatt
     7921 ttatatggta aagcagtagt gagtggtcat tttgaaaaga tcaacggaca ggacttagca
     7981 aaattccgtg ttacgtaagc cgggactgat gctaagtaaa attatgaaga tgaagataat
     8041 tttatagctg ggctttattc atcagattga aaagttgctg agtagcgatt tatctacgag
     8101 aatatattca ttaagacgcc ttatcgccaa ctccatataa taattatggc attaaaacta
     8161 cttcagattt cttctaaatt gttctaaaag aaagtcatac ttaatttttg acgctgttga
     8221 tgttgaattc agtataacac agtcaattga tcggccctta aaatatattt taaataaagt
     8281 ttcagattta acttggtcaa tggcagaagc ctcaattcct ttttgctgaa tgtatcttat
     8341 aaatgatctg gcagtactat taatagctga ataaccaggt aatagaattt tctttagctc
     8401 aggatgattt tcgataatag aaatgatatc gtgatattta ttaattctga tatgagtatc
     8461 tagagcactt ttacccattc tctcaatttc gaaacctaaa ttttgtacgc caatttccaa
     8521 cttcttcatt atttcatatc tctcttgaac tgcctttgtt ttatcagact tcgtccattt
     8581 taaattttgt tccgctaaat cggctaatat tttccaaaat ctattttgcc cattaggata
     8641 gaaaaaggga tattcccatt tagaaggatg aggtggaaag cttccaagaa ttaaatatcg
     8701 catcggagaa acatccctat accaatttgg atgctcttct ttattccaat ctgtatccat
     8761 aaactttaag ccttaggctt gtatataatt gtaaatagcc gtaattgcag tcacagttta
     8821 taactagtac tcaatagaaa actgtaccag ctgtaaaaat tatataccca ctaaatactg
     8881 tccctgtata tactttgcta cttctgtcag gcttttagtt ttttcaaaaa cagccaactg
     8941 ccggtcggca ccggtaccgt tttccagcat cttatgtaca tgggcaatac ggtggcggct
     9001 acccaggtgg tcaagaaccg ggtcgaggaa atctaacaac tcgtagataa gtgcccttgt
     9061 atttacttct tcttctttgc caaaatcaat aagataaccg tccactccat agcggctggc
     9121 cctccattta ttttcattta gcaacgctct tgagtattga ataaaattca ggttcttgga
     9181 acgaagcata tatatcctgg cacagatcgc ctggaataaa gcagccaggg tgatggtttc
     9241 atccacagtg agtggtatat cacatacacg gaactcaaca gtgttaaaga atgggtgcac
     9301 ccgcaggtcc caccatatct ttttggcatt atcaatacag tttgttttga tcagcagctt
     9361 cacataatta tcataagctt ctatactgtc aaacgcatcg ggaatacccg tacggggaaa
     9421 cttatcaaat actttggtgc gaaaagattt ataacctgtc attctacctt cccagaaggg
     9481 agagttggtg ctgagggcaa atacatgcgg caaaaaataa cgggtactgt tggctatatg
     9541 attggccatc tcccggcttt ccatacctac atgcacatgc aggccaaaga ttaaattgct
     9601 tcttgcagct tcctgcagtt cgttcactat ttcactatag cgaacatgat ccgttatcag
     9661 ctggctttcc caatggctga atggatgggt gccggatgct cccatggcaa aacccagccc
     9721 atgggcaata ccaccaattg ttttgcgcag ggcagcaaca tccgtaaacg cttcatcaat
     9781 atctgcacag atatctgtcc ccacttccac cacggcctgg tgcatttcag ccttcacttt
     9841 atcctttatc attttctgtc cttcctgcac aatcttctgc tcatgacttt tcaactcata
     9901 ggttgcaggg tcaagtacca tgtattcttc ttctacaccg agagtaaagc ttttataatt
     9961 gagattcata tttcagttta tagtttgtgg tttgtagtgt atagttggtt ctccgcattg
    10021 tgtaaagatt cttcgatttg ctttattgct aattacaaat cacaaactat agactacaaa
    10081 ctaaattata tcaaaggact tttattggaa ccccttctta catattcacc ccaggtaaga
    10141 ttatcagcac catcaacctg gctcaatgct ttttcaatcg catagttggc tgaagtttcc
    10201 accacccatt caaaattttc ctgtccaacg ctgtgcacat ctgcatcagg cgcagggtta
    10261 caaaaatcaa tggcataagg caccccatca cgaatagcca gttcaaccgt attgaaatcg
    10321 taacccaggt atttattgat acgtaatacg atatcagtca tctgttgtaa gcgttcaggt
    10381 gatggtttga agtctgctac atagcgcaga tggtgagggt tacggggttc gtaggacatg
    10441 atgcggacat atttaccccc gatacaatag cagcggtaat attctgtaaa gacaatttct
    10501 tcctgcaaca gcattaccaa ctggcctgtt tcagcatgtt tggcaaaaaa gtcttcagca
    10561 ctgtcaagcc tgtacacatg tttccatccg ccaccggcaa aaggtttcat atacgcaggg
    10621 aatccaacgt agttgaatat agtatcccag tcaagcggga aagccaggtt gctgaaagaa
    10681 tcgttggatg tgtcatccgg cagatccctg gatggaatga ttgccgtttt aggaacaggt
    10741 acatccactt ttgtcatcag gcagttattg aaatatttat catctgcact ccaccagaag
    10801 ggattgttga ttacggcgca accggttaca gctgcatttt ttaaccaggt gcggtaaaaa
    10861 ggtacgtcct gtgagatacg gtcgaagata acggcataac cactgggttc cccctgcatg
    10921 gccttgtcaa tacgtacatt ctccgccatt ataccgggca catttttgct gtttactctt
    10981 tcaataaagg cctcaggaaa tgaacgttcc ttaccatgca gaattccaat tttcttcata
    11041 agcaattttt aattgaatgt ttttaaatgt aaaccgttcc ggggctttgc ggaatatggg
    11101 aagcggcttt tccaaattta ttttttcggc atcaaataaa caaattaacc gggtggtttt
    11161 accagttgtt tttaaaatat tcaaccaaag cgctgtttat tatgaaatcc ttatttttga
    11221 aatcatgcac aagaccgcaa aagcgtccat tattcgtact gaaaacgttt tcctgaaatc
    11281 ccagttcctg caaagggagg tttgtattga tttgtttgtc ccggctgctg tttctactga
    11341 tgctccggta agtctcttac tcatcaatga cggtcaggat atggagaaaa tgaatttcca
    11401 taagatactt gattacttgt atgcagaaca ggcgattgag cctatgttat ttgtggccat
    11461 acatgccggg gaggacagga aaatggagta tggtacacag aaccacctcg attacaaaga
    11521 ccgtggcgcc aaggcaccat tatacacttc ttttattttt gatgagttgc ttccttatat
    11581 aagacagaaa tatgccattg cctcgtttaa agaaaaagct tatgcaggct tttcattggg
    11641 cgggctgagt gcattggaca ttgtctggaa ccggcccggc gaatttacca aagtgggtat
    11701 attctctgcc tctttatggt ggagaagtgt agaccaggat gatgaggaat atgacgatga
    11761 taaacacaga attatgcacc agcagataag aaacgggcaa tttgctccct ggctgaagtt
    11821 tttcctgcag tgcggtaacc tggatgaaat taaagaccgt aatcacaacg gcattattga
    11881 ctctatagag gatacattgg atctcgtaaa agaattagaa aataaaggat atgacaggga
    11941 gaatgatatt tattacctgg aactggaaga tggccatcat gatgtgtata cctggggcag
    12001 ggctatgccg gtgtttttga agtgggggtg gggattgaag taaagattga atttagaagt
    12061 agattaaaaa gtatcaataa aacaaaaatc ccgtctggtt attccaaacg ggatttttta
    12121 ttgtacttat ttcaatatta gttgaagata tacctcaaac caaactgaac agaataagta
    12181 gaggcaacgg ttacgttatc cctgaatgtc ctggtgatga tatctccctg tgctgtagcc
    12241 agtctgaatt gtggtactac agaaccaccg ggtatcaatg aattctggtt tgttggaaca
    12301 aggattgaac ttgcattgat tgatttcagc ttaccccagc ttggattgat catattacca
    12361 aagttgaaga tatcaagagt gaactgaatg gtattcttgc tcttgccaac attagcaaaa
    12421 atgtcctgca tgaacttcag gtcaacccgg ttcaaccagg gaatttgtgc accattacgt
    12481 tctgcatatt gtccacggtg agctttcagg tatttgtcct gcaaaatgta agactcaaac
    12541 aggttacgct gtgctgtttg gtcgtaaaca acaccattaa cggtttggct ggcaaactgc
    12601 atctgctgta cctgagctgc tgttggaata tatatcaggt cattaccagt aacaccatct
    12661 ctattaaagt cagcaccata tacataagag aaacggcctg caataccacc attatagatc
    12721 atggagatgg tagtagcgag atgtttaaaa tattctttac ggtaagaaac taaaccaact
    12781 acacggtcag gaagtacata atcagcataa cccattggtg aggtgttagc gccatttaca
    12841 gtagcagttc cctgccatgc agaaagaggc tggtctccac ctccatcaaa caaattgcct
    12901 gcaagtgatt tggtgtaagc tacggaagca taaaatcctt tgctgaaggg cttttcaaat
    12961 ttcgcagtca atgagaaata ataacccctg ctgccattgt cgagtacaat agagttgaaa
    13021 gctgtggtcc cgtttgtaac aaactgccct gcactattta atgtattgat aaaccttgtt
    13081 ggtacagttg atccatagat agccctgtta tccggataac cagacacatt taaagcagat
    13141 ggagatataa gatttgcatt tctaaaaaat gcagtattga ggtctttggt gaataatgct
    13201 tcaagagttg caacgatacc acccggtaat tttgtatcca ttgcaagcgt tgacttccat
    13261 gactgtggat tcttgaaatc aggcgacaaa gctgtaatag agccgggaac gatagtgccc
    13321 gctgttggaa cagtagcagg gcggtaagct gcaggatccg ggttgaatgg gccaggcgta
    13381 ttagactgac cattgaatgc ctgggtaatc tgcaacattc cattatcacc agactggcta
    13441 actatccata caaacggaac tcttccggta aagacacccg taccaccacg gatttgtaag
    13501 gaacggtcgc catagatatc ccagttgaag ccaaacctgg gagaccacat aatcctttta
    13561 tcaggcaggt tcccggtatt aattttttca ccaccagcaa aagttctttg cgctacaagt
    13621 gggtgagtaa tgatctgcgg aacatcagga taagtaggca gatccaaacg taaaccaaca
    13681 gttaaccgga agtttttgct aagagaaatt tcatcctggc cataaacaga gtattgggca
    13741 aatttgaacg ctgagaaagc aggtgcaaaa tcttttgaaa gtgagtaggt gatagcgaaa
    13801 tctctggggt taacacgcag tgcaggatta gggtttaagg cactggcaaa atcagcccag
    13861 gtattaaata cataataact ggtagcgaaa cgttggaaac cattaatggt ttcgctcagg
    13921 tcaaactggc cacccactgt ccagttatgt ttgccggacg tccaggtcag gttatcaata
    13981 atcgaatatg tttttacttt tctaaggtta ccgaagctga atggctcata accaaacgaa
    14041 gtataagggg aggaagcgga tacacctgtt gtacttaaaa tgtctacaaa aggaaatacc
    14101 tggctttctg actcacggga gtcattctga taggtataag tgccacgtaa agtattggca
    14161 attcttcccc agctggagtt tagttctgca gcaatagaat acaaattagc tccctggaaa
    14221 tagtttgaat tcttatacca gagtgagttg gtaccggtac gggtcagccc acttgggtag
    14281 ttaaagccag caccggaagt tgaagtactt ggagggttgg gttcaccacc ctctacctgg
    14341 ctgtagcgaa tattaaaacg atgtcttgaa ctgatattcc agtccaggcg aagcatatat
    14401 ttagttcttt cgatatcagg tgtatagcca tcaaaaggac cagttacata tccgtagtta
    14461 ttaaacaggt actggctgat aaaattaagt gagtcagccg atggtcgtac tatattatca
    14521 gagcttccga atggcctggt tgcagaagag gcaaatcttg tctgaattgt ttttggttgt
    14581 ttttctgttt cgtagttgaa gaagaagaac aatttgtttt tgatgatcgg gccacccact
    14641 ctgaaaccat attgcttgaa ttcttcagca ggtcttgtga agtaggtttt ctcaaccctg
    14701 cggccacgat gtttttcatt tctgaaatag gtataaacag atcctgtaaa ggtattggta
    14761 ccggaacggg ttacagcatt gattgcacta ccgataaaac cggtctgacg gatatcaaat
    14821 ggagtaaggt taacagaaat ctcttcaagc gcatcgagag aaattggtgc accgccggca
    14881 ggcaggttac ttccaattcc aaagctgtta ttaaagtctg aaccatctac agtaaagttg
    14941 ttctgcctgt agttaccacc gccaatggag gcaccgttag attgcggggt agcccgggta
    15001 agatcattga tacttctgct gatagtaggc agttggttga tctggcggat accgatattg
    15061 gttactgatc cggttctgct cgcattcagg atagagcttc ttcttccggt ggtaatcact
    15121 acattctcaa gagaagtagt tgttttagtt aaaaggggat tcaataagaa tgattctgcc
    15181 agctggagat aaatattatc atatttatcc acatcatgac caacataact tatttcaatc
    15241 aggtaagggc cacctacccg catattttgt atagaaaact ggccacctgc acgggataca
    15301 gttgaatatt ttgtacctga aggctggtga gtggctacga ttgttgcacc aacaaggggc
    15361 tcatcggagt tgtttttcac cgcaccatta atggcagagg tagttatctg ggcaaatagc
    15421 aaagggctgg ccacaagggc tactacaaac aataagacta ttctcttaag cataaagatt
    15481 ggttttcggt tgcaaagaaa gggaatattt ccaaattaaa tataggcaaa tattaacaaa
    15541 ctgctattga aatggaatgc tttacaaaga cagtcccttc cctggattcg ctgccctgtg
    15601 atctttaaaa cttagcaccg gtttgtttgt tctatttttg ctaaaaatca cgggaatgtt
    15661 tgattctatt gaaagcgcca ttgcagacat aaaattggga aaattggtgg ttgtggtcga
    15721 tgatgaagac cgggaaaatg aaggagattt tgtaacagcc gcccggaatg tgaccccgga
    15781 agtcgtcaat tttatgagca aacacggaag gggcctcatt tgtgccgcat tgcccgaaga
    15841 acgctgcaat gaactggggc tggaactgat ggtaaataat aatactgcgc tgcacgaaac
    15901 cgccttcact gtatcagtag atctgctcgg tcatggttgt accaccggta tttccgcaca
    15961 ggacagggcc aaaacaatac aatccctgat agatcctact ataacacctt gcgacctggg
    16021 gcgtcctggg catattttcc ccttaagggc taaaaagggc ggggtgcttc gcagggctgg
    16081 ccatactgag gcagctgttg acctggcccg attggcaggt tttgaaccgg gtggagtgct
    16141 ggtagaaatt atgaacgagg atggtaccat ggcccggcta cccgaattaa cagccgttgc
    16201 ccggaaattt gatttcaaaa ttatctctat caaagacctt attgaatacc gggtaaaatt
    16261 ggattcactg atagaagaag tggtaagagt ggatatgcca acccagtttg gaaattttca
    16321 gttggtggca tttaaagaaa agaactcatc caatgagcac ctcgctttgc tgaaaggaac
    16381 ctgggaaaaa gatgaaccgg tgatggttag ggttcactcc tcctgtttta ccggcgatat
    16441 tttgggctca ctccgttgcg attgtggcga acagttacat aaagccatgc aaatggtgga
    16501 gacggaaggg aaaggtgcta tattatatat gaaccaggaa ggcaggggca taggcctgct
    16561 gaataaactt aaagcatacc ggttgcagga gcagggcatg gatactgtgg aagctaatct
    16621 tcacctggga ttccagatgg accagcggga ttatggtgtt ggcgcacaaa tgctcaggca
    16681 tctgggaatc actaaactcc ggttaatgac aaacaatccg aaaaagaggg taggattgat
    16741 tgggtatggt cttgaaatag ttgaaaatat ccccatcgaa atttgtccta ataagcacaa
    16801 cgaaaaatac cttcagacaa aaagggataa aatgggacat gaaattttag ataaggaata
    16861 agaatggtgt tcctgcctat aaattatcgt tctcttttat ttcttcctct ttcttaagct
    16921 cagtctcggc aggaacaacc tgtacttttt tctttcttct gaaaaggtca ctgagtttat
    16981 caaagtcttt cttataagag atgctggcac ccgttcttct tgcagtggaa gcattggaag
    17041 atgaagtgag gtaatcggta ttggtacggt aaaagaaagt agccctgatt gttccgcttg
    17101 ggtttatcag ccattccaat gttacatccg gaagcaattg gatattttgc tgaatattgg
    17161 attggctgcc ctggcttagc ggggcgtcaa atcctacacc ggtggaaata ataaaacgat
    17221 tattgaaaaa agagcgtcca atggacaggt ttacattact gctcaggtta agggcagttt
    17281 tacttgcatc aaccaggttt ctattgtaaa tggatgtatt caggttgatt ttatatttat
    17341 cacttttcaa caggttcccg aataatttat tgatctggtc actcaataca cctaaaagta
    17401 ttccggatat ggtactgacg ccaaggccgc caccgctgct cacatccccg gccagttcac
    17461 tgggggcaaa actgttgaag acgataagat aagtcacctg cctgtttagt tcattcgtat
    17521 ttttttgcat ctgctgaact aataatgcta attccgggtc agtagtagct acacttgtac
    17581 ttggaaaatc cagtgcaaaa tttatatccg gtgtaaataa tttaccagta agtgtggcta
    17641 ccacaaacac atcacctctt gcattgctga tacccgaggt aagatttaag gagctggcta
    17701 atggtgcaaa acttactttc tctgccctgt acgtggcttc aaacttaata ttcgcattat
    17761 agggatcacc gttccactca atatagttct gtactccttt ccttatctca aatggctttt
    17821 tgaaaaatga ttgaaaagta aacaggtaat tcccttcctc tatatcaaaa cggccacgta
    17881 atgataaggg ttctgttgta cccgaacgaa tattaagtgt gcccgaacct tttcctttta
    17941 tctcatcacc ggtaagttca tccaatacca cttttacggt aaccatgggg ttagctgtta
    18001 cttccacatc atagataata ctggtttcat ttgacttaaa atcttcatcc gtcatttccc
    18061 gtccgtattt tctttccacc aggaagtcag caatgccaga ttcacggctg gatgaagaag
    18121 gtaaggtaat gtaactgctg tcttcctcag aggcattggc attaatggtc atcaccatat
    18181 cagattgcgg acctctgagc tgtaatacgg ctgttccttt tacatcgcca aaaaactgtt
    18241 tattatcgtt gtacgttgta tttaatattt gtaccgggcg gtttgtcgca tcacccctgg
    18301 tacccggttt tcgggtggat atatcaaggt catagaacat attcttgaat gattcatgat
    18361 caataccacc tgttatataa accgggttac cggtaacagt gtcggtaaga acgattccat
    18421 ccaggtctat tttctctgta gtaagttcga tgtctgtatc ctgaatttta taatagcatt
    18481 gtgtaaaatt cactcttagg ccggcatctt ttacttttcc tttgccactt actcccaggt
    18541 tatcaaactc acccgttata ttaatatctc cggtaaggaa gccctgtatg tcggagaaca
    18601 aggttccaag aaagcgttcc aggatcttta aagggaaagt ttttgctttt aaagctataa
    18661 tgttgttttt tgcttttaat gagtcgccga agaatagatg aaggttaaat ccaagataat
    18721 tttcctggtt cagtgtattg ccgtttactt tcaattcttt ggaaacatta tcataagcca
    18781 gggtggcttt caattctccg atcgagtcgt tatccagccg caggaaccgg gtctgtatat
    18841 cttcagaagt tatttttaag tggcccgtcg ggtcttctac cagaatgttt cctgataata
    18901 aaccctccag cctgtttctg gggagaaaga agggagcaaa gtcgttaaga tttacattca
    18961 ctagtgctac cttcagatcg ctccagtcgc caatatctga tggtttggtc ttaagtaata
    19021 ttttttgttc gccttctgtc agtacgagtt caccctgtgc cggattattt tttctgaata
    19081 tcagttctcc ggtttcgtca atggtccata gttttccatt tactgtaaat gaagaagggt
    19141 caatatctat tttcactcca tcattatatg ttaatactaa agcgttcagg tctgcttttt
    19201 ccacagtctg gttggccccg gtcaggatag acacttttga tgagtcgtta cgggcattta
    19261 tcttaaatac cgttattggt atattaagac tgtcgtttat ttttatattg gctgttttgc
    19321 ctgtaagaac aaggctgtcg gcatttcctg cagctattaa gctaacatca tcaaaattgt
    19381 attgcttgta atgaaattgc ggaatgtcgg cttttaggtt cagctcattt ctggcaaggt
    19441 tcaggttgcc ctcaaagtga ctgttgttca ggcctgttat tcccggtata aacattttaa
    19501 agtattcctc tacatagtac gtatagatat cgaattttaa atcctggtta ccgggaaatc
    19561 ttccgggtgg ctttatatat gcaggataat atttattcag gaaataagta aaggcgtttg
    19621 gcaggtcttt aataacaaaa tcgcctgtga cagaagcggt aaattcattg gaaacggctg
    19681 ttaatttttt aacactgtct atgtaagtgg aggaaaggat caatgaatca aatggaagac
    19741 ggattccatc tttcttaatt tctgcttcgg tgattcttgc atcgcccagg aaattatcaa
    19801 tcgtactgct ggtgaaatca aaattcaatt ttccactgaa ctcaatacta tcttttgtca
    19861 gtctcaggtt tttgaaattg gctttggtaa cgtcagccag caaattaaaa cggggagtta
    19921 ggctattaaa atctatgacc ccgttaaggt cgactttcac attctcatca ttgatggtgg
    19981 cagcaccatc aaaaagcttt ttatcgagct tccctttaac tgttatatta ttataccggt
    20041 agttgttgta gtctgcaaat ttgatcgtgc catccactaa tgtatttctt gttttctcac
    20101 tgaagccttt ccctttaacg gtgccggtaa gggatacaat accaatattt ttgtctccta
    20161 aaaattcgcc cagcctgaaa ttatcagttg caattgatcc cgaatatacc ggatcctctc
    20221 ctttgggcag tttcatgttc aggtcacttt ttatagtgcc catgttggta ttaatcgttc
    20281 catatgttac aaaatcgctg atgaaaccag taaagctgcc tttaaaattt acatattgga
    20341 ttttccggag atcaggattt gttatttttt ttaatcccgg gactatttga atggcatcgc
    20401 tgtaagttgt tctgaaatcg ttggctttga aatcaataaa tgtctggtta atatcaggaa
    20461 ggcctgtcaa tgttatgtct ccgttaagta cggtgctgtt gccggccttc accaccattt
    20521 ctcttccgat aagatcatct actgtacctc ttacttttcc ctgtagtgta atttgttttt
    20581 tccatgtttt taaagaggga gcaaagaaag cgatatcatc actgtctatg taagactcat
    20641 caaatgttgc agccatcttc actttatgaa taaaatcacc catatcactc atgtcatcgt
    20701 aggacattga aaagtaatgg cgcaggttac ttctgttggt gctcaggtca aggttagtaa
    20761 atgccattcc ctgcggtgtc attttcatat cagcggaaag attctttact tccagtccgc
    20821 ttctttcttt agccgtcaat ttcaattttg caaaaactgt atcaccaata aaagttgaat
    20881 tattcaggtt ggcgttgatc tctgtaaata atatatgctg cccgtcaaaa tgagcatagg
    20941 cttgcctgtc agtttctttg ttggtactga atgtgccgtt gatgattttc agatcggcaa
    21001 ttttgaaaac ggtttttgct ttattccagg ttgccgaagt atcagcttcc ccgtctgtta
    21061 ttactgaagc aaaaacaggt tta
//
LOCUS       DAORTI010000051        20940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2119_length_20940_cov_6.961216, whole genome shotgun sequence.
ACCESSION   DAORTI010000051 DAORTI010000000
VERSION     DAORTI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20940
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_20940_cov_6.961216"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1379)
                     /locus_tag="PLO70_08420"
     CDS             complement(117..1379)
                     /locus_tag="PLO70_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQZ74529.1"
                     /translation="MNTQVPLAERIRPRTLDELMGQEHLVGKGSILRTAIENNKVHSM
                     IFWGPPGTGKTTIANIIAHTLQAPFYQLSAISAGVKEVREIIEQAKTKTGAVLFIDEI
                     HRFNKGQQDALLGAVEKGIVTLIGATTENPSFEVNSALLSRCQVYVLKALDENDLIKL
                     LQTAIEKDEYLSKKKIELKETAALLNISGGDGRKLLNLLEIVCDTAGEKIVITDKYVM
                     EVAQKKIALYDKSGEQHYDIISAFIKSIRGSDPNAAVYWLARMTEGGEDVKFIARRML
                     ILASEDIGNANPTALVMANAAFDAVNKIGYPEARIILSQCAIYLAASPKSNASYAAIE
                     AALAAVRKEGDLPVPLHIRNAPTKLMKDIGYGKNYEYSHSYNNNFSPQEYLPGKIAGT
                     KFFDPGKNAREEELRKVLKNLWKDKYNY"
     gene            complement(1444..1842)
                     /locus_tag="PLO70_08425"
     CDS             complement(1444..1842)
                     /locus_tag="PLO70_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74530.1"
                     /translation="MKKLILSIIAAGFMLLAFSATASAQKKINVKVESKAVDANRGSN
                     PKIKSEAPTTDVVEAKSRSTCSVYFDNYSGLYVKVYVDGYYRGTLSPYGSFTVNVADG
                     YTEIYCISTGGTRDWSASGNCTGRYIYSLY"
     gene            complement(1965..4214)
                     /locus_tag="PLO70_08430"
     CDS             complement(1965..4214)
                     /locus_tag="PLO70_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ74531.1"
                     /translation="MTKNIFTLAFASLLTFSGFSQEKRALIIAIDKYEPPPNTRIAGT
                     ARSLFKNLDGCVNDGRAIQSIVVSKFQFPANKVDTLFNEAANRNGIIQSMNDLLEKSN
                     ANDFAFIYYAGHGSQVPNSLAREEDKKDESMVPSDTWKPGVQDIRDKELAKVYNAFLD
                     KGVKLTVIMDCCHSGSLSRGPNTPGQFRFIADANYDAKDASQPTPPETRPEGNFLIIS
                     AAQDNEFAQEQRDENNIPHGAFTIALRQALEQQSVNASVINLFTSIRAILKSNGKKQE
                     PVLGGKPERQQQTLFGITKGTLPDKSLIAVSGFEFERVFLQGGFALGLNKENELVKFK
                     GTDTLVKLEIDSVYGVNKSRARIVKGNIKDLKAGELLEVTNWVSSSAPLLKVYIPAGK
                     MNYSAITRLAEINNELRQSKKIKWVNELEKNDPYTTLYFDGEKYRVNIDGKEVAAPGS
                     LNTAAILQLCKQDSSFYFELPVPGELTEAIKMRLQSSKNKSIVVVNNIADAQYVLYGT
                     IDENGKPAYGLRRTQTSARDSLESMPVQTKGFVLEDGSNQAAMSVSENLYEYAMRLSK
                     IRGWIQLIGPKEGESNFPFHLEMKNKTTGSTITNNEYRVGEQVAFHLVANDGYTGANK
                     VKRFVYVFIIDKDGNMTLAYPDADAGNVGNQFPKFENFNLVKDVFLFEGTVSEPVGTD
                     NYFLLASDEPITNYAMVFNQEGVRSAARGDGSPLGDLLNIGNEGGTRGFNKSVSNWNL
                     IKLSVKSRY"
     gene            complement(4714..7167)
                     /locus_tag="PLO70_08435"
     CDS             complement(4714..7167)
                     /locus_tag="PLO70_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ74532.1"
                     /translation="MLNRFIIFSFCTLLFLQANAQPDERGFKKSSDTTGKKGDTYGII
                     IGISDYKVVPDLQFAHKDAQAFEEFLLSDAGGKVPRVNVETFLNENATRNNVADAISI
                     IARKAKSGDRVYFFFAGHGDMEDLTQIENGLLLLYNSPNGNYFGMKDDVLEILDLKRY
                     LSPLSEKGVEMIFIVDACHSGNLNGGIKGIERTASALASSWGKEYKILSCQPNQLSLE
                     STEWGGGRGLFSLHLEEGMKGLADRNNDSKVSFSELERYIKDNVETYSEEKQIPLIVG
                     DLSKPFVNVNSSVLTALKIQKAKDRPALARVNPKGMEEKYIDSLEPEGKKIYLSFQRN
                     MADKKLIWPKDTNALKDYRFFSQRYADNPLTPLMRRNLAAALNQRFDSIVSPLLRGET
                     SYSSKDVCYYAAMELDSCMQLLGQQHYMFPNLKARKLFMEAMSLTWALSESEYNIGLQ
                     PAVEKSLQLLEESETLEPNAAYTLYALGTHYFFVYDYEKAFVKLQKYLDLRPNNFWAK
                     FSLGQLYMKLKEFNKAAIIFEDLIKLHPAYTELYRLLSEAYSNDNKPEAARQCIRQIL
                     VAAGDTANYYFYTAVYFAKSNQPDSAIYYYRLTQNLYGVCTVCDNNIGHMYMVNNKTD
                     SARYYFNKVLAADSTVPFSHFNIGTIETLEKNFYKAIDRFISTIDYSTGGREGYVTRM
                     DLYFNKEYTVTDSSLFREFSTKSYIFNMQYLSWLSILYCYLRDEQLLAMKDKQEMVFN
                     WLFKYKEFDVWTWYHHTCWKALLQEKTVALESLEKSLKLGFGNYFQLTSDKDLDYIRD
                     TPEFNALLKKYFPGERSKK"
     gene            7323..9545
                     /locus_tag="PLO70_08440"
     CDS             7323..9545
                     /locus_tag="PLO70_08440"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ74533.1"
                     /translation="MRLFLDAMKNRSVVLMLVGLFSSLVSLGNPGIVVGATYVLAKSQ
                     YPQVLKKHDPVAIYIVYKGALIGSNWRNTFFRGDTIINLKGFIDSTKGGLRAYRLIFG
                     SEENIDSAWQMDNKFRVTDKVFNLGNNIVRVFAYDSTQQVTDKFVFNYALKFKETGKQ
                     VPSLSRLLREEIVFTISDRNKFSVGAAWILSIGINRYEGKAISPFANCESDAISYNTF
                     FKKKYLEKFGNRDSGFNYNEYLLTGKNATKEAILNALKDIAAKAAPNDFFVFNFSGFS
                     NPVSFDSSNYFTYFFPYDVNGYAKDFVSYTDRYQEEVSDKLISLKVLQEHIQVIPAIN
                     QLFITEAGPTKNFKTEFIRMLMQNSPTVAGILNKNRVIIVPNGFGRDDFRCNDSVIRK
                     GPINYFITSLDSTLNIYDLFTEGHKSGEIVFNLKNREVECRSFGYSYFDVFFEKQFLQ
                     QYNEIFGDERAETRGMKVRPKEMQQEVAGLTGKRYALVVGTDNYRGNGWKKLSNPIKD
                     ARAVADEFANSYGFEVQLLEDKPMDTIYKAIREYYLIAKPNDQLVIYFAGHGDVDDEL
                     LDDGFIVCSDSKSVDNDPVRNTYIPYQKLQKMLNNIPARQVLVLLDVCHGGVFDQKIF
                     EKEKREGLSGILSNKNVLQFLKDKLPLRTRKFLSSVGSEPAFDGKAGRHSPFANLLLQ
                     VLRAKGAGSNGIVTLTDIFRVLQTASMNETATLKISPAMADFGNVDAFSEFIFIPVEK
                     "
     gene            complement(9613..9684)
                     /locus_tag="PLO70_08445"
     tRNA            complement(9613..9684)
                     /locus_tag="PLO70_08445"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9649..9651),aa:Glu,seq:ttc)
     gene            9923..10936
                     /gene="gap"
                     /locus_tag="PLO70_08450"
     CDS             9923..10936
                     /gene="gap"
                     /locus_tag="PLO70_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQZ74534.1"
                     /translation="MSTVKVAINGFGRIGRLAYRQIYNMEGIDVVAINDLTSPKVLAH
                     LLKYDSAQGRFDADVKSTEDSIVVNGDILKIYAQKDPSQIPWASHDVDVVLECTGFFT
                     DKEKASAHLKAGAKRVVISAPATGDLKTVVFNVNHSILDGTETIISCASCTTNCLAPM
                     AKVLNDKYGIVTGIMTTIHAYTNDQNTLDAPHAKGDLRRARAAAANIVPNSTGAAKAI
                     GLVLPELKGKLDGSAQRVPTITGSLTELTSILNKPVTIQEINAAMKEAANESFGYTED
                     EIVSSDIIGIHFGSLFDATQTKVMTVGDKQLVKTVSWYDNEMSYVSQLVRTVNYFAKL
                     IGK"
     gene            10994..11350
                     /locus_tag="PLO70_08455"
     CDS             10994..11350
                     /locus_tag="PLO70_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74535.1"
                     /translation="MKIKNIEGLSAYDLQQEAEKGGKFVYFAFTISLLVITFKRTSGV
                     YLVRKGERIVSKGLPFTIISALFGWWGLPFGPKYTLESIRTNLNGGKDVTDEVMATVA
                     GHILFRETQAEKAHQH"
     gene            11380..12576
                     /locus_tag="PLO70_08460"
     CDS             11380..12576
                     /locus_tag="PLO70_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQZ74536.1"
                     /translation="MSKFAAHDFKGQKALIRVDFNVPLNDKYEITDDNRMRAAVPTIK
                     KILNDGGSVILMSHLGRPKDGPTEKYSLKHLIQHLSELLGGTTVLFANDCIGEQAYLT
                     AGMIRPGEVLLLENLRFYKEEEKGDKVFAEKLSKLGDLWINDAFGTAHRAHASTAIIA
                     EFFPAEKRMFGLLMEGEVASAEKVLHKSEKPFVAIIGGAKVSDKILIIENLLERATDI
                     IIGGGMAYTFMKARGGKIGNSLCEEDRLATALEILQKAKEKNVSIHLPEDSVIADKFD
                     ANANTAVAPSDAIPDGWMGLDIGPKAAGIFTEVIKKSKTILWNGPMGVFEMEKFQKGT
                     RAVAVAIAEATESGAFSLVGGGDSVAAVNQFGFADKVSYVSTGGGAMLEYFEGKVLPG
                     IAAVQS"
     gene            12761..13354
                     /locus_tag="PLO70_08465"
     CDS             12761..13354
                     /locus_tag="PLO70_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQZ74537.1"
                     /translation="MKGTRNLTILVVLGLLLILGVWGCNGYNAVIKQDETVKKTWNNV
                     NTEYQKRSDLVGNLVNTVKGAANFEQETLTKLVEARAKATSINLTADQLTPENMAKFQ
                     EAQGQMSGALSRLLAVVENYPDLKASQNFLQLQGQLEGIENDVRNSRKLFNDAINTYN
                     TKVRSFPVNLLAGMFGFKAKEGFKADEGAEKAPKVEF"
     gene            13366..13956
                     /locus_tag="PLO70_08470"
     CDS             13366..13956
                     /locus_tag="PLO70_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQZ74538.1"
                     /translation="MQQKKFSGYIVGGAILLVLAIWIITSYNAFVNKQEKVKLQWSEV
                     QNTYQRRIDLIPNLVNVVKGVSDFEQTTLEKITEARSKAINGLSDNEISGENYQKQKQ
                     LQDTLAAAANRLIIQIEKYPALKGTTAYAGLQTQLEGTERRIKVARNDFNEAVAEYNK
                     KVRGFPSNLVAVIFGFKKKEGFVADTGTEKTVEIKF"
     gene            13974..14435
                     /locus_tag="PLO70_08475"
     CDS             13974..14435
                     /locus_tag="PLO70_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQZ74539.1"
                     /translation="MKLFSWNKNKSLFNEEDNRLIVKAIRQAEKRTSGEVRVFVERHC
                     RFMDAIDRAAEVFFSLQMEKTEQRNAVLVYVALKDHQLAVFADEGIYKQTGQDYWNQI
                     VTEMIQSFNKDYYAKGIAECVIQIGEALTTHFPFDSDTDKNELPDHIVFGS"
     gene            14441..15202
                     /locus_tag="PLO70_08480"
     CDS             14441..15202
                     /locus_tag="PLO70_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQZ74540.1"
                     /translation="MKRLLLILTLHFSLFAAAQMEKIIVTPPSPPKLVNDYTKTLTSQ
                     QVEALEGKLVKYDDSTSNQIAVVIVESTDSYSIEDAAIELGRKWGVGNKDFNNGIVIL
                     VAKADRKMTIQVGYGLEGAITDLTSKSIIDNTITPRFKEDNYYRGLDEGVDDLIAAAE
                     GRYKAPANYGSKKNKGLSTWTVIFIIILLLVIFGSGAAGGGTYVSRGGFGGWTGRGSG
                     WSGGGGWSGGGGSSGGGFGGFGGGSFGGGGSSGSW"
     gene            complement(15275..17794)
                     /locus_tag="PLO70_08485"
     CDS             complement(15275..17794)
                     /locus_tag="PLO70_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQZ74541.1"
                     /translation="MKKVLFTLLLILPGFVLLAQTKPSADTAWKKEYRETATKINNLV
                     HTKLDVKPDFSKSYLYGKAWLTLKPHFYSTDSLTLDAKGMEFKKIALLKGNQQVPLKY
                     EYDDWQLRIKLDKSYKAGESYTIYIDYIAKPDEFEEKYAKGAMLGIKGMYFINPKGEE
                     KDKPTQIWTQGETESSSAWFPTIDKTNQKTTQELTVTVDNKYVTLSNGKLVSQKKNKD
                     GTRSDYWKMDLPHAPYLFFLGVGEYAVIKDSWRGKEVNYYVEKEYASVAKKIFGNTPE
                     MMTFFSKITGVDYPWIKYSQITGRDYVAGAMENTTATIHQESAQQDARELVDGNNWEG
                     TIAHELFHQWFGDYVTTESWSNLTVNESFADYSQTLWDEYKYGKDAGDAENFNGMQGY
                     LMSGSENKQLVRFHYKDKEDMFDAVSYQKGGRILHMLRNYVGDSAFFKSLNNYLTTNK
                     FKSAEAHQLRLAFEDVTGRDLNWFFNQWYFNSGHPSVDIDYQYDDAAGKVNVIIKQTQ
                     KSGKIFSLPLAIDVYDGANKKRYNVWVKNGVDTFTISYAKRPDLVNVDGDKVMLWVKK
                     DNKTLDNYIHQYTHAGNYLDRREAIDFASKKLDDPKAVELIKTALTDKYHGLRNFAIS
                     KADLKKETVKTGFESVLLNLAKSDKYATVRASAIQKLGDYKKAEYADLFKKAVTDSSY
                     TVAGNALAALIKVDPATSSALVKQLAEKPSKGILQEVVMSEILKSGDESMAEKIIGDF
                     AKMPLSQGKFQALNGLATYLSAIKNAEKIKWGVDEIVKFRDAIPENFKNQTDPFINGM
                     VLKGILADKTKKAKENAADTALQELVEYIKAKLPEADKKGF"
     gene            17950..19359
                     /locus_tag="PLO70_08490"
     CDS             17950..19359
                     /locus_tag="PLO70_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74542.1"
                     /translation="MNKLLLVFLFAATSLTTSAQKVYFVYLQSEPEQPFFVKMDDKIY
                     SSSASGYLILAKLRDTSYSFKVGFPQNKWPEQVFTVAVNRKDHGFLLKEFGDKGWGLF
                     DLQTLAVQMSATGIAKVADKVKTDNKDVSAFTEILSKASDDPSLKEKQVQQKPEEKPA
                     PKTESVAVEVIKKQETKKEIREPAVIKTADVVTIKAEKKEDPKTAIKEPAITKSAEII
                     DSIEVNKEEGKVVIEEVPVSKPVEPVVELAIKKEDPKIVSEQPVPVSETPKESLEEEY
                     TRSVVTKRSESSTTEGFGLVFIDNYENGTRDTIRLLIPNPRLIVGVEMAEPKEEKKFL
                     DIVTEAPKKQEEKPVEPQPKMEEKPVAAETPVEKPVALVNCPATADENDFFKLRKAMA
                     AAEGDEEMVAEAKKYFKIKCFTTAQLKNLSTLFLNDEGKYNFFDAAYKHVSDKASFIT
                     LQSELKDEYFINRFKAIIR"
     gene            19407..20177
                     /gene="truA"
                     /locus_tag="PLO70_08495"
     CDS             19407..20177
                     /gene="truA"
                     /locus_tag="PLO70_08495"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQZ74543.1"
                     /translation="MTRYFLELSYKGTDYSGFQLQDNANTVQAEVEKAFKILQKHRLV
                     MTGSSRTDAGVHALQNFFHFDYEGKINPHFIYKMNAILPSDIVVKSFQKVASDAHCRF
                     DAIGRSYNYYIYQHKDPFLKDRAFFFPYKLDFDKMQRAAAIVKEYHDFTSFSKRNTQV
                     KTFNCLIQESEWLSENNNLVYHVKANRFLRGMVRALTATMLKVGRGKLSLEEFRAIIE
                     SKDCTKASFAVPAHGLFLISVVYPPDYLAINSKYGHEK"
BASE COUNT         6199 a         4001 c         4307 g         6433 t
ORIGIN      
        1 gatggtgttg tatcagattc aagtgagcca ttattggaag gggtgccgcc ggtgatgaaa
       61 ggagagaaaa tgcagggaca atttttcccc ttgctttttg atcccaggct cataaatcaa
      121 taattgtact tgtccttcca gagatttttc agcacttttc ttaattcttc ttccctggca
      181 tttttacccg ggtcaaaaaa ttttgttccg gctatttttc ccggcaaata ttcctgtggc
      241 gaaaaattat tattgtaact atgggagtat tcataatttt tcccgtagcc tatatctttc
      301 atcagtttgg tgggggcatt gcgtatgtgt aacggcactg gcagatcacc ttctttccgg
      361 actgctgcca gtgccgcttc tattgcagca taagaagcat tgcttttcgg agatgctgcc
      421 aggtatatcg cacactgcga caaaataatc cttgcttccg ggtagccaat cttattaaca
      481 gcatcaaatg ctgcatttgc cattaccaaa gctgtgggat tcgcattacc tatatcctcg
      541 ctggccagta tcagcattct gcgggcaatg aacttaacat cttcaccacc ctctgtcatc
      601 cttgccagcc aatacacggc agcgttggga tcactgcccc tgatcgattt gataaaagcc
      661 gatataatat catagtgttg ttcaccgctt ttatcataca acgcaatttt tttttgcgcc
      721 acttccatta catatttgtc ggtaataaca attttttctc cggcagtatc gcaaacaatt
      781 tcgagcaggt tgagcaattt tcttccatca ccacctgaaa tgtttaataa ggcagccgtt
      841 tcttttagtt caattttctt ttttgataaa tattcgtctt tctcaatagc tgtttgcaaa
      901 agtttgatga ggtcattttc atccagcgct tttagtacat aaacctggca cctgctaagc
      961 aaggcactat ttacttcaaa tgatggattc tcagtggtgg caccaattaa tgttacgatt
     1021 cctttttcaa ctgcacccaa cagcgcatcc tgttgccctt tattgaaacg gtgtatctcg
     1081 tcaatgaata atacagcgcc tgtttttgtt ttagcctgtt caatgatctc tctcacttct
     1141 tttactccgg cactgatggc actaagctga taaaaaggag cctgtaatgt atgagcaatg
     1201 atattggcaa tcgttgtttt gccggtacca gggggtcccc agaagatcat cgaatgcact
     1261 ttgttatttt ctatagcagt tcgtaaaatg cttcctttcc ctaccaggtg ttcctgcccc
     1321 attagctcat ccagtgttcg gggacgtatt ctttctgcca gtggaacttg cgtattcata
     1381 ttgccaaagt aacaaaaaaa agcaggcgac tccgcctgct tttttctgtc aattgttgaa
     1441 agatcagtat agcgagtaga tataccggcc ggtgcaatta cccgatgccg accaatctct
     1501 tgtaccccct gtagagatac aatatatttc agtataacca tcagcaacat ttacagtgaa
     1561 agaaccatag ggtgataaag tgcccctgta atatccatcc acataaactt ttacataaag
     1621 gcctgaataa ttatcaaaat aaacactgca ggtacttctt gatttggctt ctacaacatc
     1681 agtagtagga gcttcacttt tgatttttgg gttagatccc ctgttggcat caacggcttt
     1741 tgactcaact tttacattga tttttttctg ggcagaagcg gtggcagaaa aagcgaggag
     1801 cataaatcct gcagcaataa tgctaagaat taattttttc atgcttttta gttttaaatt
     1861 ttgaaaactt gattggtatt aacttaatga gccagtatgc ttaataataa tattataagt
     1921 tactaaatct ctatatataa tacctgctta tccgtattta atttttaata cctggatttg
     1981 actgacagtt taataagatt ccagttagat actgacttat taaatcctct tgtaccacct
     2041 tcattaccaa tattaagcaa gtcacctaaa ggactaccat caccccgggc ggcggacctt
     2101 acaccttcct ggttaaaaac cattgcataa ttggtaatgg gttcatcaga tgccagcaga
     2161 aagtaattat ctgttccaac aggttctgaa acagttccct caaacaggaa aacatcttta
     2221 acaaggttaa aattctcaaa tttcgggaat tggtttccca cattgccagc atcagcatcc
     2281 ggataggcta acgtcatatt cccgtcttta tcgatgatga aaacatacac gaaccttttt
     2341 actttattgg cccccgtata tccatcattg gcaacaaggt gaaaggctac ctgttcacca
     2401 accctgtact cgttattggt aatggtgctg cctgtggttt tgtttttcat ctccaggtga
     2461 aaagggaaat tactttcgcc ttcttttggc cctattaatt gtatccagcc ccggatcttg
     2521 gacagacgca tggcatattc atacaggttc tcagaaacag acatggcagc ctggttacta
     2581 ccatcctcca gtacaaaacc cttcgtttgt accggcatag attcaagact atcccgtgca
     2641 gaggtctgtg tacggcgtaa tccataagcc ggtttgccat tctcatctat agtgccatat
     2701 aaaacatatt gtgcatcggc aatattattc acaacaacga tacttttatt tttagaagat
     2761 tgcaatctca tctttatcgc ttcggtcagt tctccgggta cgggtaattc aaaataaaag
     2821 gatgagtcct gtttgcataa ttgaagaata gcagctgtat ttaaagatcc cggtgcggcc
     2881 acctctttac catcaatatt taccctgtat ttttccccat caaaatacaa ggttgtataa
     2941 ggatcatttt tttccagttc attcacccat ttaatttttt ttgattgtcg cagttcattg
     3001 ttgatttcag caagcctggt gatagcactg taattcattt tcccagctgg tatatacact
     3061 ttaagcaaag gtgctgatga ggaaacccag tttgttactt ccagaagctc accggctttt
     3121 aagtctttga tgtttccctt tactatcctt gccctggatt tattgacacc gtaaacagaa
     3181 tcgatttcca gttttaccag tgtatccgta cccttgaatt ttaccaattc attttcttta
     3241 tttagtccca gtgcaaagcc tccttggagg aacactcttt caaattcaaa acccgaaaca
     3301 gcaatcaatg atttgtcagg gagtgtgccc ttagtgatgc cgaacaaggt ttgttgttgt
     3361 ctttccggct tacctcctaa aacaggctct tgttttttac cattactttt caggattgca
     3421 cggatactgg taaataaatt gataacggag gcatttaccg actgctgttc aagtgcctgt
     3481 ctcagggcaa tggtaaaagc accgtgcgga atattatttt catctctttg ttcctgtgca
     3541 aattcattat cctgtgcggc cgagataatc aggaaattac cttccggcct tgtttccggg
     3601 ggggtgggct gggacgcatc ttttgcatcg taatttgcat cagcgataaa cctgaactgc
     3661 ccgggggtgt tagggccacg ggaaagagat ccgctatggc agcaatccat aataacagtc
     3721 agttttacac ctttatcaag gaaagcgtta taaactttag caagctcttt gtcacgaata
     3781 tcctgtacgc cgggtttcca tgtatcactg ggaaccattg actcatcttt cttatcttct
     3841 tctctggcaa gactgttcgg cacctggctg ccgtgtccgg cataataaat aaatgcaaaa
     3901 tcattggcat tgcttttttc aagcaagtca ttcattgatt gaataattcc attcctgttg
     3961 gcggcttcat taaataaagt atctacttta ttggcgggaa actgaaattt tgaaactaca
     4021 attgactgta tagccctgcc gtcattcacg cagccatcca ggtttttgaa taatgaccgg
     4081 gcagtacctg ctatccttgt attgggaggt ggttcatatt tgtctattgc aattatcaag
     4141 gcccttttct cctgcgaaaa gccggaaaag gtcaatagtg atgcaaaggc aagggtaaat
     4201 atattttttg tcataactgg tagattaaaa gctgtaatac tttattatag cagtaaagat
     4261 ttttgttttg aacagcctgt tgaagataat tttaattgag taaagatcaa tttttctatt
     4321 aatataagta ctgtttattg tatacccgga cagcctgtca tgtatttgca catacgatat
     4381 taatattctt acgacaacga tcatatacct tcttatgggt atattactgt atgctgaaat
     4441 cattgtatat gaatcaggat aatgaatgtc tatgatcgtt ttattaccca aaataacctt
     4501 tctattcttt taatgcacca ggaatttgct tataatggtt ggctttttct gcattaagct
     4561 gagtttttag atcgtctctt ttactttaac taacaacctg cataaggtaa attctgaatg
     4621 ctaactataa atgaagaaat attactttat gaaaatatca agatgcctat cctttgatac
     4681 ctttatatat aagtttggta aagattttaa aggctatttt tttgacctct ccccgggaaa
     4741 atattttttc agtagtgcgt tgaattccgg tgtgtccctg atgtaatcga gatctttgtc
     4801 gctggtaagc tggaagtagt tgccaaaccc cagcttcagt gatttctcca ggctctccag
     4861 cgcaacagtt ttttcttgca gaagagcctt ccagcaggta tgatgatacc atgtccatac
     4921 atcaaactct ttgtatttaa acagccagtt aaagaccatt tcctgcttgt ctttcatggc
     4981 cagtaattgt tcatcccgta aataacagta aagtatagat agccaggata gatactgcat
     5041 gttgaaaata tatgactttg tactaaattc tctgaaaagg ctgctatctg taacagtata
     5101 ctctttatta aaatacagat ccatccgtgt tacataacct tccctgcctc cggtgctgta
     5161 atcgattgtt gatataaacc tgtcaatggc cttataaaaa ttcttctcta atgtttcgat
     5221 agttccaatg ttgaaatgag agaaaggaac ggtactgtcc gccgctagta ccttgttgaa
     5281 ataatagcgg gccgagtcag ttttgttatt taccatatac atgtggccta tattattatc
     5341 gcaaacagta caaacaccgt ataaattttg ggttagccgg tagtaataaa tggcactgtc
     5401 cggttgattg gatttggcaa aataaactgc ggtataaaaa taataattgg ctgtatctcc
     5461 ggcagctaca agaatctgcc tgatgcattg ccgggcggct tcaggtttat tgtcattaga
     5521 atacgcttcg gataacagcc tgtatagttc tgtatatgca ggatgaagtt tgatgagatc
     5581 ttcgaaaata atggctgctt tgttaaattc ttttagcttc atgtataact gcccaagcga
     5641 aaatttggcc cagaagttat tcggacgcag atcaaggtat ttctgcaatt ttacaaatgc
     5701 tttttcatag tcatacacaa agaaataatg agtacccagc gcatataagg tgtacgcagc
     5761 attgggttcc agcgtttctg attcttccag taattgaagg gatttttcaa ccgctggttg
     5821 caggccgata ttatattccg attctgacaa ggcccaggta agggacatgg cttccataaa
     5881 caattttctt gctttcagat taggaaacat ataatgttgc tgacccagca attgcatgca
     5941 gctatccagt tccatggccg cataataaca aacatctttt gaagaataac tggtttctcc
     6001 tctcagtaaa ggcgaaacga tagagtcaaa acgctggtta agtgctgcag cgagattgcg
     6061 gcgcatgagt ggtgttaacg ggttatcggc atagcgttga gaaaaaaacc tgtagtcttt
     6121 cagggcattt gtatcttttg gccagataag ttttttatct gccatgtttc tttgaaaaga
     6181 gagatatatc tttttgcctt caggctccag ggaatcgata tacttttctt ccatcccctt
     6241 tggatttacc cgggctaatg ccggccggtc tttggccttt tgaattttta aggcggtcag
     6301 taccgaagag tttacattca caaatggctt gctgagatcc cccactatca gggggatttg
     6361 tttttcttca ctgtaggttt ctacattgtc ctttatataa cgttccagtt cagagaagct
     6421 cactttgcta tcattatttc tgtcggcaag tcctttcata ccttcttcca ggtgcaacga
     6481 aaataaaccc cgtccgccac cccattcggt actttccagg gagagctggt tgggctgaca
     6541 ggaaaggatc ttatattctt taccccatga cgaagctaat gccgatgcag tcctttctat
     6601 ccctttaatt ccaccattta aatttccgga atgacaggca tccacaataa atatcatttc
     6661 aacacctttt tcagaaaggg gagataaata tctttttagg tcaagtattt ccagcacatc
     6721 gtctttcatg ccgaaataat taccattggg actattatat agcaggagca ggccattttc
     6781 aatttgtgtc aggtcttcca tatcaccatg accggcaaaa aagaaataaa ccctgtcacc
     6841 ggatttagct ttgcgggcta tgattgaaat ggcatcggcc acattattac gggtggcatt
     6901 ttcattaaga aaggtttcca cgttgaccct tggtactttg ccaccggcat cactaaggag
     6961 gaactcttca aaagcctggg catctttatg agcaaactgc aggtccggca ccactttata
     7021 atcactgata ccgataataa taccataggt atcacctttt ttgccagtcg tatccgatga
     7081 ttttttaaat cctctttcat cgggttgagc attggcttgc aggaaaagca atgtgcaaaa
     7141 tgaaaatatg ataaaccgat tcaacattat ttttagaatt ctattttcat attgtgtaat
     7201 aaattattac cagtactcat aaatcaattt aaagataata cagtttggta aagaagccgc
     7261 tacccgcacc agggtattgg tgaactccct taatagctat accttttcag taaattatgt
     7321 taatgcgatt atttttagat gcaatgaaga acagatcggt tgtattaatg ctggttggat
     7381 tattttcttc tcttgtttct ttgggcaatc cgggaattgt ggttggcgca acctatgtac
     7441 tggcaaaaag ccagtacccg caggttttaa aaaaacatga tccagtggca atatacattg
     7501 tatataaagg agcgttgatc ggcagcaatt ggagaaatac tttttttcgt ggtgatacta
     7561 ttattaacct gaaaggtttt atagattcaa caaaaggagg actaagggcc taccggctca
     7621 tttttggaag tgaagaaaac attgacagtg cctggcagat ggataataaa ttccgtgtga
     7681 cagataaggt ctttaatttg ggtaataata tagttcgggt ctttgcgtat gactcaacac
     7741 agcaggttac agataagttt gtttttaatt atgcactgaa atttaaagaa acaggaaagc
     7801 aggttccgtc gttaagtcgt ttattacggg aggagatagt ttttactatt tcagacagga
     7861 ataagttttc agttggtgct gcgtggatac tttctattgg tatcaacagg tatgaaggaa
     7921 aagcaatcag tccctttgcg aattgcgaat cggatgctat ctcctataac acatttttta
     7981 agaaaaaata cctggagaaa tttggtaacc gggattctgg tttcaattat aacgagtatc
     8041 ttctgacagg gaaaaatgct acaaaagaag caattctgaa tgcattaaaa gatattgcag
     8101 caaaagctgc acctaatgat ttctttgttt tcaattttag cggttttagt aaccctgttt
     8161 cctttgattc ttccaattat ttcacttact tcttccctta cgatgtgaat ggttatgcta
     8221 aggactttgt gtcctataca gaccgttacc aggaagaagt cagcgataaa ctaatttcac
     8281 tgaaagtgct gcaggagcat atccaggtga taccggctat caatcagtta tttattactg
     8341 aagcggggcc aacaaaaaat ttcaagacag agtttatcag gatgttaatg caaaattctc
     8401 ccactgtagc aggtatcctc aataaaaaca gggtgatcat tgttccaaat ggatttggga
     8461 gggatgattt ccggtgcaat gattcagtaa ttagaaaagg gcctattaac tatttcataa
     8521 cttccctgga tagtacatta aatatctatg atttatttac tgaaggtcat aagtccgggg
     8581 agattgtttt taaccttaaa aacagggaag tggaatgccg gtcgtttgga tacagctact
     8641 tcgatgtgtt ctttgaaaag caatttctcc agcagtacaa tgagatattt ggtgatgaaa
     8701 gggctgagac aagggggatg aaggtcaggc caaaggaaat gcaacaggaa gtggcaggcc
     8761 tgacaggtaa acgatacgct ttggtggtgg ggactgataa ttacagggga aatggctgga
     8821 aaaaattaag taaccctatt aaagatgcaa gggcagtggc tgatgagttt gccaattcgt
     8881 acggatttga ggttcaatta ctggaagata aacctatgga tacaatatat aaggccatcc
     8941 gtgagtatta tcttattgca aaacccaatg accaactggt aatttatttt gccggccatg
     9001 gtgatgttga tgatgaattg cttgacgatg gttttattgt ctgctccgat tcaaaatcag
     9061 tggataatga tcctgttcgt aacacttata taccttatca gaaacttcag aaaatgttaa
     9121 acaatatacc ggcccggcag gtactggttt tattggatgt ttgtcatggc ggggtgtttg
     9181 atcagaaaat atttgagaaa gagaaaaggg aagggctttc cggaattctt tcaaataaaa
     9241 acgtattaca gtttttaaaa gataagttac cgctgagaac aagaaaattt ctgtcatctg
     9301 taggttcaga accagctttt gatggtaaag cgggtcgtca ttctcctttt gccaatttac
     9361 tattgcaggt attgagagca aagggagcag gttcaaatgg aattgttacg ctgacagata
     9421 ttttcagggt actacaaaca gcaagtatga atgaaacggc gacattaaag atatctccgg
     9481 ctatggcaga ttttggaaat gtggatgcct tcagtgagtt catttttatc ccggtcgaaa
     9541 aatagttacc aataaagtga acggaaataa attaaaaaga ggttcagatt aagtatctga
     9601 acctcttttt tgtagcccgt acgagattcg aactcgtatc accaccgtga aagggtggtg
     9661 tcctaaccct tagacgaacg ggccgtttta agggacggca aagataggtt tgcacaactt
     9721 ttttgccaaa tgtttttcgc tttgtttttc ccacatcctg ttcaggcttt ttaaccctca
     9781 aaatcaccct tctttttcac cctattagtg taccttcgca ccgcaaatta aaattgcaaa
     9841 accggcttag ttttcttaaa aaagctggat gctaagccct taaaatttca gttttttaaa
     9901 ctcttaaact tttaaaccta ccatgagcac agttaaagtt gccattaatg gctttggacg
     9961 tatcggacgt cttgcttacc gacagatata taatatggaa ggaattgatg tggtggccat
    10021 caacgatctt accagcccta aagtactggc ccatctgctc aagtatgaca gcgcccaggg
    10081 tcgcttcgat gccgatgtaa aatctaccga agattcaatt gtggtaaacg gtgatatatt
    10141 aaagatatat gcacaaaaag atccttcaca gattccttgg gccagccatg atgtggatgt
    10201 ggtgctggaa tgtaccgggt tcttcaccga caaagaaaaa gcatctgcac acctgaaggc
    10261 tggcgcaaaa agagtggtga tctccgctcc tgcaaccggc gatctgaaaa ccgttgtctt
    10321 taatgttaac cacagcatac ttgatggcac tgaaactatc atttcctgtg cttcctgtac
    10381 taccaattgc ctggctccaa tggccaaagt gttgaatgat aaatacggga ttgttacggg
    10441 tattatgact acaatccatg cgtataccaa tgaccagaat acactggatg cgcctcatgc
    10501 gaaaggtgat ttacgcaggg caagggcagc agcagctaat attgttccta actccaccgg
    10561 cgctgcaaaa gctattggcc tggtattacc ggaattaaaa ggcaaactgg atggtagtgc
    10621 acaacgggtg cctactatta ccgggtcttt gacagaactt acttctatct taaataagcc
    10681 cgttacgatt caggagatca atgcggcaat gaaagaagca gcgaatgaat catttggcta
    10741 cacagaagat gagattgtaa gcagcgatat catcggtatt cattttggtt ccctgtttga
    10801 tgcaacacaa acaaaagtta tgacggtagg agataaacaa ctggtaaaaa cagtaagctg
    10861 gtacgataat gaaatgagtt atgtaagtca gctggtaaga acagtgaatt attttgctaa
    10921 gttgatcggt aagtaatttt ttattaccgc agtaaactgg tcatcagttt gctgcggtat
    10981 tttaagcggc tttatgaaga ttaagaatat tgaggggctt tctgcatacg atttgcagca
    11041 agaagctgaa aaaggaggaa aatttgtata cttcgcattc acaatatcgc ttttggttat
    11101 aacattcaag cgtacctccg gcgtctacct ggtgaggaaa ggggaaagaa tagtatcaaa
    11161 ggggctgcct tttacaataa tctcggcttt attcggatgg tggggacttc cttttgggcc
    11221 aaagtataca ttggagagca ttcgtaccaa tttgaatggg ggtaaagatg ttactgatga
    11281 agtgatggct accgttgcag gtcatatatt atttcgggaa acgcaggctg aaaaagcaca
    11341 tcagcattag cctgattcat tcatttcaaa aaaaatacta tgtcaaaatt cgctgctcat
    11401 gattttaaag gtcagaaggc acttatccgt gtcgacttta atgtaccact gaatgataag
    11461 tatgaaataa cagatgacaa ccgtatgagg gctgctgttc ctacgattaa aaaaatattg
    11521 aatgatggtg gaagtgttat cctgatgagt cacctgggaa gacccaaaga tggccccaca
    11581 gaaaaatatt cattaaaaca tttgatacaa cacctgagtg aattattggg cggaacaacc
    11641 gtattgtttg ccaatgattg tattggcgaa caggcttatt taactgcagg aatgattcgt
    11701 cccggcgaag tattgttgct cgagaacctg cgtttttata aagaagagga gaagggagat
    11761 aaagtgtttg cagaaaaatt atcgaaactg ggcgatctgt ggataaatga tgcttttggt
    11821 acagcgcacc gggctcatgc ctctacggct atcattgctg agtttttccc ggcagaaaaa
    11881 aggatgtttg gattattaat ggaaggcgaa gttgccagcg ctgagaaagt attgcataaa
    11941 tctgagaaac cttttgtagc aataataggc ggcgcaaaag ttagtgacaa gatactgatc
    12001 attgaaaatc tactggaaag agctactgat attatcattg gcggagggat ggcctatact
    12061 ttcatgaaag caaggggtgg taaaatagga aactctttat gtgaagaaga ccgccttgca
    12121 acagcgcttg aaattctgca gaaggcaaaa gagaaaaatg taagtataca tcttccggaa
    12181 gattctgtta ttgctgataa atttgatgca aatgcaaata cagccgttgc acccagtgat
    12241 gctatacccg atggatggat gggacttgac attggcccca aagcagctgg tatattcaca
    12301 gaagtaatca aaaaatcaaa aaccatttta tggaacggcc ccatgggtgt ttttgaaatg
    12361 gaaaaattcc agaaaggtac ccgggctgtg gcagttgcta tcgcagaagc cacagaaagc
    12421 ggagcctttt ccctggtagg tggcggcgat tctgtagctg ccgttaacca gtttggtttt
    12481 gcagataaag taagctatgt gtctaccggt ggtggtgcca tgcttgaata ctttgaaggc
    12541 aaggttcttc cggggattgc tgccgtgcaa tcttaaaaca cagaagggtt gcaatacccg
    12601 taaaaaatat aaaaatgtcc ctgtttaaaa tcaggggctt ttttgtgacc agtccggctc
    12661 ttgatgatca tttaccggct gtcagctgct gtatgccgaa aatcagcgaa tggcaataac
    12721 aaaattccta tttttaatat ctaaacaaaa ccccattaac atgaaaggaa cccgtaatct
    12781 caccatctta gttgtacttg gcttgctgct catactgggc gtatggggat gcaatgggta
    12841 taatgccgtc atcaaacagg atgaaacagt taaaaaaaca tggaacaacg taaacactga
    12901 atatcagaaa agatctgacc tcgtcggtaa tcttgttaat acagtaaaag gtgccgctaa
    12961 ttttgaacag gaaacattga caaaattagt ggaggcccgt gccaaagcca cctctatcaa
    13021 tctaacagct gaccagttaa ctccggagaa tatggcaaaa ttccaggaag cacagggaca
    13081 aatgtctggt gcattgagcc gcttgctggc tgtagtggaa aactatcctg atctgaaagc
    13141 cagccaaaac ttcctgcagt tgcagggaca attggaagga attgaaaatg atgtccgtaa
    13201 ttcaagaaag ctttttaatg atgctatcaa tacgtacaat accaaggtaa gatcattccc
    13261 ggtgaacttg ctggcaggta tgtttgggtt caaggcaaaa gaaggtttca aggcggatga
    13321 aggtgccgag aaagctccaa aagtagaatt ctaagaatag ctgatatgca gcaaaagaaa
    13381 ttttccggct atatagttgg cggtgctatc ctgcttgttc ttgctatctg gataatcact
    13441 tcttataacg cttttgtcaa taaacaggag aaggttaaac tccaatggtc agaggtgcaa
    13501 aacacttacc agcgcaggat tgatctgatc ccaaacctgg tgaatgtcgt gaaaggggtt
    13561 tcagattttg aacaaactac cctggagaaa attacagagg caagaagcaa agcgataaat
    13621 ggtttgtcag ataatgaaat atcaggtgaa aattatcaaa agcaaaaaca attgcaggat
    13681 acattggcag ctgctgctaa ccgattgatc attcagattg aaaagtatcc ggcactaaaa
    13741 ggtacgaccg cttatgctgg tttgcaaaca caactggaag gaacagaaag aagaataaaa
    13801 gtggcccgca atgattttaa tgaagcggtg gcagaatata ataaaaaggt aaggggtttt
    13861 ccatctaacc tggtggctgt tatttttgga ttcaaaaaga aggaaggttt tgtcgcagat
    13921 accggaactg aaaaaacagt ggaaattaaa ttttaactat taactataat ccgttgaaac
    13981 ttttttcatg gaataagaat aagtcgctct tcaatgaaga agacaaccgg ctgatcgtaa
    14041 aagcgatccg ccaggcggag aaaaggacca gtggagaagt ccgggtattt gtggaaagac
    14101 attgtcgttt tatggatgca atagacaggg cggcagaagt cttcttttct ttacaaatgg
    14161 aaaaaacaga acaacggaat gctgtattgg tctatgtggc ccttaaagac catcagttag
    14221 ctgtatttgc tgatgaagga atttataaac agacaggcca ggactattgg aatcagatag
    14281 ttactgaaat gattcaaagc ttcaacaaag actattatgc taaaggtata gcagaatgtg
    14341 tgatacagat aggagaggcg ctgacaactc atttcccctt tgatagtgat accgataaaa
    14401 atgaattgcc tgatcatatc gtatttggca gctgattttc atgaaaagac ttttattaat
    14461 actaaccctt catttttctt tatttgctgc tgcgcaaatg gagaaaatta ttgttactcc
    14521 tcccagccct cctaagcttg tcaatgatta tactaagaca cttacttctc agcaggttga
    14581 ggcactggaa ggaaaacttg taaaatacga tgactcaact tctaaccaga ttgctgttgt
    14641 tattgtagaa tctaccgata gttattccat cgaggatgca gccattgaac tgggcagaaa
    14701 gtggggagtg ggtaacaagg attttaataa tggtattgta atacttgttg ccaaagcaga
    14761 caggaagatg accattcagg ttggatatgg gttagaggga gccattaccg atttaacatc
    14821 aaaaagtatc attgataata ccattactcc ccgctttaag gaagataatt attacagggg
    14881 tttggatgaa ggcgtcgatg atttaatagc agctgcagaa ggcaggtata aagctccggc
    14941 aaattatggt agcaagaaga ataaaggtct gagtacctgg acagtaattt ttatcatcat
    15001 tctcttgctg gtcatttttg gttctggggc ggcaggcggc ggcacttatg tttcccgcgg
    15061 tggatttggt ggctggacag gccgtggtag tggttggtca ggtggtggcg gatggtcggg
    15121 cggaggagga agcagtggag gtggttttgg aggctttggc ggtggtagct ttggcggcgg
    15181 tggttctagt ggtagttggt aagaagaaac tcgattaaat acaaaaagcc atcctattga
    15241 aaggatggct tttaatttta caggattagc gctactaaaa tcccttctta tctgcctccg
    15301 gcagttttgc ttttatatat tcaaccagtt cctgcaatgc agtatcagca gcattttctt
    15361 ttgccttttt ggtcttatcg gcgagaatac cttttaatac cattccatta ataaacggat
    15421 ctgtttgatt cttgaagttt tcaggaatag catccctgaa ttttacaatc tcgtctacac
    15481 cccatttaat cttttcagca tttttaatag ctgataaata ggtagccagg ccattcaatg
    15541 cctggaattt accctggctt aaaggcattt ttgcaaaatc accaatgatc ttttctgcca
    15601 ttgactcatc cccggacttt aatatttcac tcatcaccac ttcttgcaaa ataccttttg
    15661 aaggtttttc agccagttgt tttaccaatg ctgatgatgt ggcaggatca actttaatta
    15721 aagcagccag tgcatttccg gcaactgtat aggaagaatc ggtaaccgct tttttaaaca
    15781 ggtcggcata ctccgctttt ttataatcgc ccaatttttg gatagcgctg gctctcactg
    15841 tggcatattt gtcacttttg gccaggttaa gtaatacaga ttcaaatcct gttttcactg
    15901 tttctttctt tagatctgcc ttactgatag caaaatttct caggccgtga tatttatctg
    15961 tcaaggctgt tttaattaat tccacagcct tgggatcatc cagttttttt gacgcaaagt
    16021 caatagcttc tcttctgtca agatagttac cggcatgtgt atattggtga atataattat
    16081 caagcgtctt gttatctttc tttacccaga gcatcacttt atctccgtcc acgtttacca
    16141 ggtcaggcct ttttgcataa ctaatagtaa aagtatctac cccattttta acccaaacat
    16201 tatagcgttt tttatttgcc ccatcatata catcaattgc cagcggtaag gagaatatct
    16261 ttccgctctt ctgtgtttgt tttataatga cattcacttt tccggcagca tcgtcgtatt
    16321 gataatctat atctacactg ggatgaccac tgttaaaata ccattggtta aagaaccagt
    16381 tcaggtcacg gccggttacg tcttcaaatg ccaggcgcag ctgatgggct tcggcagatt
    16441 tgaatttatt tgttgttaga taattattca gtgatttgaa aaaagcactg tcgcctacat
    16501 agtttctcaa catatgtaaa atgcggccac ctttctggta actcaccgca tcaaacatat
    16561 cttctttgtc tttgtaatga aaacgtacaa gttgtttatt ctcacttcca ctcatcaggt
    16621 acccctgcat gccgttgaaa ttttctgcat caccggcatc ttttccatat ttatattcat
    16681 cccaaagtgt ctggctgtag tcagcaaacg attcgttcac tgtcaggttg ctccagcttt
    16741 ctgtagttac ataatcaccg aaccattggt gaaaaagctc atgtgcaatg gtcccttccc
    16801 aattatttcc atccactaat tctcttgcat cctgctgtgc actttcctgg tgaatggtgg
    16861 cagttgtatt ttccatcgct ccggctacat aatcacggcc tgttatttgt gaatacttta
    16921 tccatgggta atcaacaccg gtaattttgg aaaagaaagt catcatttcc ggggtattgc
    16981 caaatatctt tttggctaca gaagcgtatt ccttttcaac atagtaattt acttctttgc
    17041 ctctccagct atctttaata acagcatatt cacccacacc taaaaagaaa aggtagggcg
    17101 catgcggcag atccattttc caatagtcac ttctggtgcc atctttattt ttcttttgtg
    17161 atacgagttt gccgttactg agggttacat atttgttgtc aacagttacg gttaattcct
    17221 gggtggtttt ttgattggtt ttgtcaatag taggaaacca ggccgaactg gattctgttt
    17281 ctccttgtgt ccatatttgt gtgggcttgt ctttttcttc acctttagga ttgataaaat
    17341 acataccttt tattccaagc attgcacctt tggcatactt ttcttcaaat tcgtcaggct
    17401 ttgcgatgta gtcaatatat atggtataac tttcaccggc tttatagctt ttatctaatt
    17461 taatacgcaa ctgccagtca tcatattcat atttcagggg tacctgctgg ttgcctttca
    17521 aaagggcaat ttttttaaat tccattccct tcgcatcaag ggtcagtgaa tcagtgctat
    17581 aaaaatgggg ttttaaggta agccacgctt taccatacag gtacgattta ctaaaatcag
    17641 gttttacatc cagtttggta tgtaccaggt tattgatttt agtggcagtt tccctgtatt
    17701 cttttttcca ggcagtatca gcagacggtt tggtctgtgc aagcaataca aatcccggga
    17761 ggattaataa caatgtgaac aatactttct tcatataatt gatttgtgtg ccgcaaattt
    17821 ataataatag gcattgttta gtgatataat tgggatgaaa ggttacggta tttaacaagt
    17881 tgttgtcacc atgctgaatt atctcagggg atttaagtta aatttgctat acaacccaat
    17941 tccggaaaaa tgaataaact tcttttggtt tttctctttg cggccacttc actaacaact
    18001 tcagcccaga aagtatattt tgtttacctg caatcagaac ctgaacagcc tttttttgta
    18061 aaaatggacg ataaaattta tagttcttcc gcttctggtt atctgattct tgctaaacta
    18121 agggatacca gttatagttt caaggtgggg ttccctcaaa ataaatggcc tgaacaggta
    18181 tttacagttg ctgtaaacag gaaggatcat ggctttctgc tgaaagagtt tggagataaa
    18241 ggatggggac tatttgattt acaaacattg gcagtacaaa tgtctgcgac aggtattgct
    18301 aaagttgctg ataaagtaaa aacagataat aaagatgttt ctgcttttac tgaaattctt
    18361 tccaaagctt ctgacgatcc ttcgttaaaa gaaaaacagg ttcagcaaaa accggaagaa
    18421 aagcctgctc ctaaaacaga atcagtagct gttgaagtta taaaaaagca agaaacaaaa
    18481 aaggagatca gggaaccagc tgttattaaa actgcagacg ttgttacaat aaaagctgaa
    18541 aagaaagaag accctaaaac ggcaatcaaa gagcctgcta tcacaaaatc tgcagaaata
    18601 attgattcca tagaggtaaa caaggaagaa ggaaaggtgg tgattgaaga agttcctgtt
    18661 tcaaaacccg tggagccagt tgttgaattg gccattaaaa aggaggaccc aaagattgtt
    18721 tctgaacaac cggtacctgt cagcgaaacg ccaaaggaaa gtttggagga agaatataca
    18781 agatcagtag ttacaaaaag atcagagagt tcaacaactg aaggttttgg tcttgttttc
    18841 attgataatt atgagaatgg aacgagagat acgattcgcc ttcttattcc taatcccagg
    18901 ctaatagttg gtgtggaaat ggcagaacca aaagaagaaa aaaaattcct tgatattgta
    18961 acagaagctc caaaaaaaca ggaagagaaa ccagttgagc cacaacctaa gatggaagaa
    19021 aagcctgttg cagctgaaac accagtcgaa aagcctgtag cattagttaa ctgccctgcc
    19081 acagcagacg aaaacgattt tttcaaacta cgaaaagcaa tggccgcagc agaaggtgat
    19141 gaagagatgg tggctgaagc aaaaaagtat tttaaaataa agtgctttac tacagcgcaa
    19201 ttaaaaaatt taagtacact ttttttaaat gatgagggta aatacaattt ctttgatgca
    19261 gcctataagc atgtttctga taaagccagc tttatcactc tccaatcaga attaaaggac
    19321 gaatatttta taaatcgttt caaagcaata ataagataat aaccagcatt tcttttgtac
    19381 aactatttga aatctgactg ctaaatatga cccgttattt tttagagctt tcttacaaag
    19441 gcactgatta cagtggtttt cagcttcagg ataatgcaaa cacagtacag gcagaagttg
    19501 agaaagcttt taagattctc caaaaacata gactagtaat gacgggctcc tccaggaccg
    19561 atgccggtgt gcatgcattg caaaattttt ttcattttga ttatgaaggg aaaataaatc
    19621 cgcatttcat atataagatg aatgccatac tgccctcaga tattgtcgta aaaagttttc
    19681 agaaagtggc ttcagatgct cattgccgtt tcgatgctat tgggaggagt tataattatt
    19741 atatttatca gcacaaagac ccctttttaa aagacagggc attcttcttc ccttacaaac
    19801 tggattttga taagatgcag cgagcggcag caattgtgaa ggagtatcac gattttactt
    19861 ctttttcaaa gcgaaatact caggttaaaa catttaattg cttaattcag gaaagtgaat
    19921 ggttgtcaga gaataataat cttgtttatc atgtaaaggc aaatcgattc ctgagaggaa
    19981 tggttagagc tcttacggca acaatgctta aggtaggcag gggtaagttg tctcttgagg
    20041 aattcagggc aataattgag tctaaggact gtaccaaagc tagtttcgct gttcctgctc
    20101 atggtctgtt tttgatttct gttgtctatc cacctgatta tcttgcaatt aattcaaaat
    20161 atgggcacga aaaataagtt tcgtttgtcg ttttttgaca ataatctaaa cttatattga
    20221 gtttattcgg tttcaggctt gttcccgcat ttgtagggct ttttacatta atttaccaag
    20281 actaaggatt ttaaaacagg cctgccattc agtttaaata ttcagggcgg gatcttgata
    20341 aaaaaaaaat tgcactgaaa cctataacag cactgcattt ccttatgctt tagcataatt
    20401 aatttcaatt agagttggat agtaaatgtc acacccttat ctttgccgcc cgttcggata
    20461 aaacgggtag ttctctgaag aaatgtgaaa acaaatgtga ggcaaatgtg aacaaaactg
    20521 agcctaaaag tttgctggta atttacaata tcttaccttt gccgtccgcc tcaaaaaaaa
    20581 tgaggttagt tcttcacaaa acaagttgat tttcacgcca aaagattctg attttcttga
    20641 aaaataattg aagaaaaatt tggtagaaat aaaatggccc ttacctttgc actcccgttc
    20701 aaaaaacggg gaatgggaac tggataaaac tggttgtcat cccgagcaaa gcgagggaca
    20761 aaagatcttt gaaagttggg aaacaatagc actttattcc gatagccatc ggaataaaat
    20821 tataggtaag tcaagcgaaa tacatttcga tttgacacgt taattttctc ttgagaattt
    20881 taaaatgtca gtatcaaaca tcatttacaa tggagagttt gatcctggct caggatgaac
//
LOCUS       DAORTI010000052        20926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2122_length_20926_cov_5.985961, whole genome shotgun sequence.
ACCESSION   DAORTI010000052 DAORTI010000000
VERSION     DAORTI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_20926_cov_5.985961"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..2340)
                     /locus_tag="PLO70_08500"
     CDS             complement(190..2340)
                     /locus_tag="PLO70_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HQZ74544.1"
                     /translation="MKKLLLLLFLFSSFEYLNAENGYDLWLRYKTIDNKQLLHLYRQQ
                     ITGLTIHGSSPTITAAKEELSLGLQGLLQIKIPFTSGTHQKGTVLAGTPSSSPSIRQL
                     GLTSYLRQTGSEGFIIMTKKINGNNCIVITANTDTGILYGVFHFLRLLQTHQPVSPLN
                     IISFPRLKLRLLNHWDNLNRTVERGYAGFSIWNWHTLPGYIDKRYIDYARANASVGIN
                     GTVLTNVNANATILTEPYLQKVKALAEVFRPYGIKVYLTARFSAPIELGGLKTADPLN
                     EEVQQWWKQKTKEIYSLIPDFGGFLVKANSEGQPGPQDYKRTHADGANMLADAVAPYN
                     GIVIWRAFVYAAENPVDRHKQAYDDFVPLDGQFRKNVMVQVKNGAIDFMPREPFHPLF
                     GAMPKTPLMMEFQVTQEYLGQGTNLVYLAPLFKEVLESDTYCKGKGSTVAKVIDGSLD
                     NHSLNGMAGVANIGNDINWCGHPFAQANWYALGRLSWDYTLSSEQIAEEWLRMSYINN
                     YSFVANAKEIMMHSRDIYVQFTNPIGLHHIMGTGHHYGPAPWVSNLNRPEWNPVYYHK
                     ADSIGIGFNRTSTGSNAIAQYFPEARKQWEDINSCEEKNLLWFHHVPWNHKMKSGRTV
                     WEELCHHYYTGADRVKQMKQKWVALKPFVDKDRYEQVMQLLNVQHEEAIWWRNGCLLY
                     FQTFSKQPIPAAYEQPYKTLEYYKSLRFPFAPGN"
     gene            complement(2369..3400)
                     /locus_tag="PLO70_08505"
     CDS             complement(2369..3400)
                     /locus_tag="PLO70_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ74545.1"
                     /translation="MKQKKDITIYDIAQKLDLSSATVSRALQDHPAVNKNTRKKIQEA
                     AKELGYRHNTFASSLRKQKTNTIGVIVHELNSNFITSVLAGIEKVTTEAGYDLIIAHS
                     SESYDKEAANALNLFHKRVDGLIASLAFDTKGLDHYNLYEEKAIPVIFFDRVEENSDS
                     TKVIIDNYKCGYQATQHLIEQGCKRIVLVTANLKRNVYAQRHKGYTDALYDNNIPYEK
                     DRVLIKDLSEQCGVEAALQILKMKPIPDGAFITNDFSAAVCMRTLKEHGVRIPEDIAI
                     VGFNNDAISKLIEPQMTTIDYPGIDMGEIAARNLINHLKGVSNIKHTNTIVVRSDLII
                     RKSSLRMRK"
     gene            complement(3440..4480)
                     /locus_tag="PLO70_08510"
     CDS             complement(3440..4480)
                     /locus_tag="PLO70_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ74546.1"
                     /translation="MASDKEITIYDIAKQLNISATTVSRGLKDHPSINKHTRKKINDT
                     AKEMGYRSNTFASSLRSKRTQTLGVIVPRLNSYFVSSVLAGMEDVASKEGYNLIISHS
                     LESSEKEVANAATLFNKRVDGLLVSLAYDTENIDHLKPFFKKGIPVVFFDRTFPHHES
                     TSIIIDNAKAAYDITKHLIDKGCRRIMHLGGNVLRNVYSERIKGYKQALRDHNLPFEE
                     KLLYISKLSEDAGTQAGERILKMKEKDRPDGVFSANDTAAVHCMLTLKYAGFRIPQDI
                     AFAGFNNDPISKVIEPNLTTVNYSGYNVGEAAVSNLINHLNGVTDIKTTNTIILRCDL
                     VIRESSQKNKSL"
     gene            4848..7970
                     /locus_tag="PLO70_08515"
     CDS             4848..7970
                     /locus_tag="PLO70_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ74547.1"
                     /translation="MKKTARLKKLFFTSRQGVKPLLLIVVLLMQLLTVSQQSFAQNVT
                     VRGKITKDDGQPLSGASVVVKGTTNGTTSNEQGNFLISAPGDATLVISSIDFTSREIK
                     VNNQSTINVTLSSADKTLGEVIVVGYGTQRKEAITGSVASINGDKMREVPSPNVTQAL
                     QGRLAGVEISQTSTRPGATMQIRIRGIRSLSADNNPLIVLDGIPFIGSLADINPNDVK
                     SIEVLKDASATAIYGSRGANGVLLITTDKGSKNRKPRINYSTYVGSQSIFAKYPMMNG
                     PQFVALRAARGQYANGQDEANDVNTDWQDLFYKTGIVTDHNVSLSGGSEAGSYNFGMG
                     YYLNQGIIPTQQYKRYSLRGSIDQQVGKYFRFGFTTNNNYNESQGNQVGLYNSLSMSP
                     ISTPYNPDGSMKRIIRMAADDQFIFTKDVVEQLKDNDQWLNEDRGFASYNAIYGEVKA
                     PFLDGLKYRVNLGLDYVQFNNGAYTGQGVGSVNPASISSASVDNRFTYHWTLENLLTY
                     DHTFGGKHTVNAVALYSAEQNKYNRSNMSAQDIPADAFQFYNLGQAAGQLVINPANQD
                     YQLWGLKSAMGRVMYSYDNRYMISATVRSDGSSRLAPGKKWHTYPALSVGWNIAKESF
                     MQNITAINTLKLRVGFGQTSNQAIAPYSTLGRLDTRPYNFGPSTYSTGFYVSQLPNVD
                     LGWEFSKTLNFGLDFSAFKNRLSGTIEYYITKTEDILLGLSLPPTSGVSGYTANIGST
                     QNKGFELSLNGTIINNVNGWTWEAGVNFYTNNNKLVSLASGQTRDEANWWFVGHNINA
                     IYDYKKIGLWTDAKDSADNYLNILEPGGTAGMIKVLYTGDYDANGRPVRAIGPADRQI
                     MDVDPDFQGGFNTRVAYKGFDLGIVGIYRSGGILFSTIHGSNGYLNLLTGRRNNINVD
                     YWTPTNTGAKYPNPAGPISNDNPRYASTLSYFDGSFMKIRTITLGYDLSRSLIKNPNI
                     NMRMYFTAQNPFVMFSPFHKESGLDPETNSFGNENAAVNLSQNLRRILTVGFNTPSTR
                     NYIVGVNLTF"
     gene            7982..9958
                     /locus_tag="PLO70_08520"
     CDS             7982..9958
                     /locus_tag="PLO70_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ74548.1"
                     /translation="MKYIQIKSVFVTALFMLLFSTGCNKILDEQPRSIYEPGFFKTEK
                     GVLGGITSQYAHLRFIFGQPYYYNTLETGTDEYTWAQSADQNFKDMDLSGVGNITAAS
                     SRSDALWGTAFSNINTASGIIENASAVGISAPLIAEARFFRAFDYFLLVQTFGGVPLD
                     LGAGELKFNTTPSRTSVRNTVPEVYTKAIFPDLLTAINDLPATGRVTGGATKTLARLY
                     LAKAYLTYAWWLQNPNNIPTYPLTTRTDPDGHDAAWYFQKAYDVSVEAIDNPGPFGLE
                     NTFYDVHVGGNDRNKEMLLYADHTQESEFYNGSPLTGFQSELGGGRNSAVWMVTWNYT
                     VIRSAPNSNGTGSGVSSVQREAAQGLGRPYTRMAPPIGVFTNTFAEKTLDSRYDGTFN
                     TVFRGNWPKGGTTNATLYNANDLPVAPGDPILSFLEYDSQAAGITYSTGANFPGKSYS
                     NVGAGMLPGRADFVIAPSGISRIVFPNLWKMGVYRTGSVGLGEPNAPSTRPFKIAKFS
                     ELYFTAAEAAVKGATTVAGKSARDLINVIRARAGKWKFSNKDNAAYIADNSAAMIAAT
                     PATIDINYILAERSREFFGEGHRWFDLIRTQKWNELAGSYSICAPTYGAHTPQTFTRT
                     IEPKHYIRPIPQAQLDGMEMTPEEKAAYQNPNYQ"
     gene            10366..11631
                     /locus_tag="PLO70_08525"
     CDS             10366..11631
                     /locus_tag="PLO70_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQZ74549.1"
                     /translation="MIIKNKYFRGLQKLPVIVLILFVSCSKKGSDNNTGSSTPVLSAE
                     VTILANENKQVIQGFGCATVFAPPNTSSLINEEFDRLFGSGTGQVGLNFLRIRIASDD
                     AWRATELNHAKAAIQRGAKVFASPWSPPARMKTNNNIIGGKLIPDSGAAYARYLNDFA
                     LYMASNGAPLYAVSVQNEPDWEPNYESCVWTATEMRDFLKDQGSLVTATRLMAPELVN
                     NNQTYVNTILSDDAAAANLDIVGTHLYGGGIIENQLASNKGKEVWMTEHLDTNVTYTA
                     NLNTAIEIHECFTKANFNAYIWWYGKRFYGPIGQDGIITKRGYIISQFARFIKEGAVR
                     LGSSANSRTDVLISAYRNGIKKVVVAINLGATDVKQKIIFQGTAVTSFIPYLTTSAKN
                     AEQGSIIAISDNSFTYTIPGKSIVTLVEQ"
     gene            11909..13393
                     /locus_tag="PLO70_08530"
     CDS             11909..13393
                     /locus_tag="PLO70_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQZ74550.1"
                     /translation="MAALLLGIDIGTSSVKASVVDASTRQCIAAAQYPETESDIIALE
                     QGWAEQSADMWWEHVQLAILKLHAQRRYNPKDIAAIGIAYQMHGLVMVDKDQKVLRNS
                     IIWCDSRAVEIGDKAFEEIGREKCLLHLLNSPGNFTASKLAWVKTNEPELFARVDKIM
                     LPGDFIAMKLTGEITTSVSSLSEGVFWDFKQNELSKDVFEYYGFSTPIIPPVRDVFAE
                     HGKVLKKVAVALSLTEGIPVTYKSGDQPNNALSLNVLEPGEVAATAGTSGVIYGVSDQ
                     LTYDNESRVNTFAHVNYTTAQKRLGVLLCINGTGILNRWVKNIAGNSLSYQQINDIAA
                     SVKPGSDGMIILPFGNGAERMLKNKIVQSHIYNIDLNKHNQAHLYRAAQEGIAFAFRY
                     GLDIMRENGMHPQVIRAGKANMFLSDVFSECFVNILDIQVELYNSDGSVGAAIGSGLG
                     AKIYTDAKEAFRHFEPIQLITPNGKRAYDEFYNKWLIQLEKQLN"
     gene            13420..14748
                     /gene="xylA"
                     /locus_tag="PLO70_08535"
     CDS             13420..14748
                     /gene="xylA"
                     /locus_tag="PLO70_08535"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQZ74551.1"
                     /translation="MTVITGTKEYFKGISKIKYEGPQSDNPLAYRWYDESKVVAGKTM
                     KEYLRFACAYWHSFCGNGADPFGEPTHLFSWNEKTDAVERAKDKADAAFEFITKLGLP
                     YYCFHDVDAVDYTNDVKENDRRLQAITDYLAEKQKVSGVKLLWGTANLFSNRRYMNGA
                     ATNPDFHVLTHGAAQVKAALDATIALGGENYVFWGGREGYMSLLNTQMKREKEHFAVF
                     LHAAKDYARKNGFKGKFFIEPKPCEPSKHQYDYDSETVIGFLRQHDLLNDFSLNIEVN
                     HATLAGHTFTHELQVAADAGVLGSIDANRGDYQNGWDTDQFPNDINELTEAMLVILEA
                     GGFKGGGINFDAKIRRNSTDPEDLFHAHIGGMDIFARALITADNILNKSDYKKIRAER
                     YASFDSGKGKEFEQGKLSLEDLRAYAIEKGEPAVKSGKQEYLENIINRYI"
     gene            14768..16384
                     /locus_tag="PLO70_08540"
     CDS             14768..16384
                     /locus_tag="PLO70_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQZ74552.1"
                     /translation="MERFATLDWVVIACYFALLIGVAIWVVTRKQKNTEDYFLAGRSL
                     GWFVIGASIFASNIGSEHVVGLAGAGAGGKMPLLIYELHAWLVITLGWVFLPFYLRSG
                     VFTMPEFLEKRFNSGTRWFLSVFSLMAYILTKVSVTVYAGGIVISSLLHIDFWTGALV
                     TVVITGVYTVLGGMRAVAYTEAIQTVILVAGAATLTFIGIDAAGGWAEVKANLAPGYL
                     NMWRPNSDAEFPWLPLVITSSIVGTWYWCTDQYIVQRVLAAKNIKEGRRGTIFGGLLK
                     LLPVFLFLIPGVVALALKNKGELSWDSADQAFASLLMNKMPAGLRGLVAAGLLAALMS
                     SLASVFNSCSTLFTVDIYKKLRPNDSEEKLLKIGRMATTVVVILGILWIPIMQNISGV
                     LYEYLQSVQAYIAPPITAVFLLGIFSKRINSQGAITTLIAGLIIAILRIALELSRNSL
                     EPGSMLYSIATTNFLTFAAWFFLFCVGLCVTVSLLTKAPTPEKVMGLTYSTLSMEQKT
                     ANRASYNVWDIILSLAVIAIVAFVMISFTG"
     gene            16511..17674
                     /gene="uxuA"
                     /locus_tag="PLO70_08545"
     CDS             16511..17674
                     /gene="uxuA"
                     /locus_tag="PLO70_08545"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HQZ74553.1"
                     /translation="MEQTMRWFGPNDPVSLSDIRQSGCTGVVTALHHIPVGEVWAIEE
                     IAKRKQVIKDAGMSWTVIESLPVSEDIKKQSGNYLQHIENYKQSLRNVAQCGLKVVTY
                     NFMPVLDWMRTDISYMMPDGSRALYFERSAFIAFDLFLLKRPGAEHDYTEEEIKKARF
                     RFERMTDEEEETLFRNALLGLPGSEEQFTEEQILKALKTYEDIDAAKLKAHLYYFLQQ
                     IVPVAEECGLKMAIHPDDPPYAVLGLPRIVSTEQDAADLLQAAPSPANGLCFCTGSFG
                     ARPDNDLLKMLKRFGDHIHFLHLRNTKRDAEGNFYEADHLAGDSDMYEIIKEVLLIMQ
                     RRNISIPMRPDHGHQMLDDLKKTTYPGYSAIGRLKGLAELRGVEFGIFRSLKS"
     gene            17924..18577
                     /locus_tag="PLO70_08550"
     CDS             17924..18577
                     /locus_tag="PLO70_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74554.1"
                     /translation="MQTTIRMGKITILLVDDHKLIRDSWSFILNSDSRFQVIGETSNA
                     DEAVEISKDKKPEIVLMDINMTPVNGFEATKLVRKYSPGSKVIGISMHSMPAYARRML
                     QLGAMGYVTKNSSKDELLTAIVEVHNGKKYICDEVKNILAQQELEEEGGPPDMNVLSR
                     REIEIVQLIKEGLSSKEIALRLDISLKTVEVHRYNILKKLSLKNTAALVNFINAHGL"
     gene            18632..>20926
                     /locus_tag="PLO70_08555"
     CDS             18632..>20926
                     /locus_tag="PLO70_08555"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQZ74555.1"
                     /translation="MNRFYTRQRITIGVIISFTLVSFFAWYSYINMNKARKETLKVNS
                     TLQSLKSLENLMDDMQDIETSSRGYLISGNKEFLEPYYRALTKLRTDTVELWNLHQSY
                     PNRKEVFRSLLNLVNEKIEIASYSEKVVNENSDTAAFQLVRSGRGREVMDSIRQIIWS
                     VENDDRKVLNTSNASREIAAATTAKLFLGLAIGFIIITTLLFLQIRRDLKRREEYESR
                     ISYLAGLTEKTSDAIISIDTGLRIISWNKGAENTYGYTREEAIGKNIAELTQSEILKE
                     SDSQLHKEISVKGYVEFESKDYTKEGRSIFCQVSTTPLKNELGDVTGYVSVVKDITQR
                     KLAEKLMHEFNVELAEKVKEKTAGINRSEEKLRQVLESAASEFYVIDLEYRIILISKL
                     AELNLEKAWGKKVMTGDIVINDLPAEKLEIIRDNYNRVFRGEEIGYEAQVASDNGERW
                     VYVNYVPVKGEYGEITGAYIATKDISERKKVEKALLESEERYRTLIEQAIDCIVVTDS
                     DGKFIQVNDAGIKMFGYTREEFLQYYLKDLLVIGAGDPPIRFEELRSGIGILSYRKAR
                     RKDGTIFDLEISSKMLPNGKIIGIARDISDRMNAQRVLAESENRFKAIFNTQYQMVGL
                     LDNNGNVLEVNKTTLDITGYGNADMQGKPFWEIRFWQNERDRAERTGRVKEAIQKAAS
                     GQFVRYESAIQLPGKEIEIIDFSIKPIPDADGNIKLLIIEGRLITEIKKAENELRESE
                     AKYRAFFANSLDGILLTSPDGTILS"
BASE COUNT         6203 a         3882 c         4645 g         6196 t
ORIGIN      
        1 caatggcaaa acccaatccc gaacctcctc ctgttattat tgccactttt ttgtccccga
       61 tccctaaagg ggagttacca gcattcataa tttgtaattt cttttattaa taaaaaattt
      121 catttgttgt acttttctgg atattaatta tccttcaaac taagtttttc acccggcgga
      181 gactgggctt caattccccg gcgcaaaagg gaaccgcaag ctcttataat actccagcgt
      241 tttatacggc tgttcatatg ctgcaggaat gggttgtttg ctgaatgttt gaaaatagag
      301 taaacatcca tttcgccacc atatggcttc ttcgtgttgt acattcagta attgcatgac
      361 ctgttcatac ctgtctttat ctacaaatgg ttttaatgct acccattttt gcttcatttg
      421 ttttacccga tccgcaccgg tataataatg gtgacataac tcttcccata ccgtccggcc
      481 acttttcatt ttatgattcc atggcacatg gtgaaaccag aggagatttt tttcttcgca
      541 ggaatttatg tcttcccatt gctttcttgc ttccgggaaa tattgtgcga tggcattact
      601 gccggtggat gtcctgttga aacctatgcc aatactgtca gctttgtgat aatataccgg
      661 gttccattca ggacggttta agttactgac ccagggagca ggaccataat gatggccggt
      721 tcccatgata tggtgtaatc cgataggatt agtaaactgt acatatatat cacggctatg
      781 catcattatt tcttttgcat tggctacaaa tgaataatta tttatgtagg acatccgcaa
      841 ccattcttca gctatttgtt cgcttgataa tgtataatcc cagcttaatc ttcccaatgc
      901 ataccagttg gcctgtgcaa acggatgtcc gcaccagttg atatcattac caatatttgc
      961 cactccggcc attccattca aagaatgatt atctaaactg ccatcgatca ctttggccac
     1021 agtacttcct ttccctttac agtaggtatc actttctaaa acttctttga aaaggggagc
     1081 cagataaaca aggttcgttc cctggccaag atattcctgg gttacctgga actccatcat
     1141 caatggtgtt tttggcattg caccaaataa gggatgaaac ggttctcggg gcataaaatc
     1201 tattgcgcca ttttttacct gtaccataac attctttctg aattgtccat ccagcggtac
     1261 aaaatcatca taagcctgtt tatgtctgtc gaccggattt tcagcagcat agacaaatgc
     1321 acgccagata actattccat tatatggagc aaccgcatcg gccagcatat tcgctccatc
     1381 cgcatgggtt cgtttataat cctgcggacc cggttgtcct tcactgttag ctttcaccag
     1441 aaatccccca aagtcgggta tgagggaata aatttctttt gtcttctgct tccaccactg
     1501 ctgcacttct tcattcaatg gatcagctgt ttttaatcca cccagttcta ttggcgcact
     1561 gaatcttgcc gtgagatata cttttatacc ataagggcgg aacacctctg ctaacgcttt
     1621 tactttttgt aaatagggct ccgtcagaat agtggcattg gcattcacat tcgtaagtac
     1681 agtgccatta attccaacgg atgcattcgc tcttgcataa tcaatatatc ttttatctat
     1741 ataacccggt aatgtatgcc agttccatat actaaagccg gcatatcctc tttctaccgt
     1801 tcggttcagg ttatcccaat ggttcagcaa tctcaatttc aaacgtggga atgaaatgat
     1861 attaagcggg gagaccggtt gatgtgtttg taataaccgg agaaaatgaa atacaccgta
     1921 aaggatgccc gtatcagtat tggcagtgat gacaatgcaa ttattgccat tgattttttt
     1981 agtcattata ataaatccct ctgagccggt ttgccggagg taggaagtta atccaagttg
     2041 ccgtatagaa ggagaggagg agggtgttcc tgccaggaca gttccttttt gatgggtgcc
     2101 ggaagtaaaa ggaattttta tttgcagcag cccttgtaat ccaagactta gttcttcttt
     2161 tgcagcggta atggttggtg aagatccatg gatagtaagc ccggttatct gttgccggta
     2221 taagtggagc agttgtttat tgtcaatggt tttgtaacgc agccataggt catagccgtt
     2281 ctctgcgttt agatactcaa acgaagaaaa caggaaaagt aataacaaca gttttttcat
     2341 attcgcatat gctggttttg gccgatgttt attttctcat tctcagtgat gattttcgga
     2401 tgataaggtc ggaacgaact acaatggtat tagtatgttt aatattggat acacctttga
     2461 gatgattgat aagatttcgg gccgcaattt cacccatgtc aataccggga taatcgatcg
     2521 tcgtcatttg tggttcaatc agtttactta tggcatcatt attaaatcca acaatcgcaa
     2581 tatcttctgg tatgcgaacc ccatgttctt taagggtacg catacataca gctgctgaga
     2641 aatcattggt gataaaggca ccgtcaggaa tcggtttcat tttaagtatc tgaagagcgg
     2701 cttctacacc gcattgttca ctgagatctt ttatcagaac cctgtccttt tcataaggaa
     2761 tattgttgtc gtataacgca tccgtataac ccttatgccg ttgggcatat acatttcgtt
     2821 ttaagttagc cgttaccagt acaattcttt tgcaaccctg ttctatcagg tgctgcgtag
     2881 cctggtaacc gcatttataa ttatcaatga tgacctttgt gctgtcactg ttctcttcca
     2941 ccctgtcgaa gaaaattaca ggaatggctt tttcttcata taaattataa tgatccagcc
     3001 ctttggtgtc aaaggccaat gaagctatta agccatcaac ccgtttatga aataggttca
     3061 gcgcattggc tgcttcttta tcataacttt ccgaggagtg tgcaatgata agatcgtatc
     3121 cggcttctgt agttactttt tctatcccgg ccaacacaga cgtaatgaag ttactgttca
     3181 gttcatgcac aatgacacca atggtgtttg tcttttgttt acgcaggctg ctagcaaacg
     3241 tattatgacg ataccccagc tctttggccg cttcctggat ttttttcctg gtgtttttat
     3301 ttaccgccgg gtgatcttgt aatgcacggc tgacagtggc ggatgaaaga tccagtttct
     3361 gtgcaatatc atagatggta atgtcttttt tctgtttcat ggtgcaatcg gttgcataaa
     3421 aatcgcaaaa aaaatcaatt tacaaagatt tgtttttttg agaagattca cggatgacca
     3481 gatcacaccg gagaatgatg gtgttggtag tttttatgtc agtaaccccg ttcaaatgat
     3541 tgatcaggtt gctgacggca gcttctccca cgttataacc agagtaatta accgttgtca
     3601 ggtttggttc tattacttta ctgatggggt cgttattgaa accggcaaat gcaatatcct
     3661 gtggtatacg gaaaccagcg tattttaatg ttagcatgca atgtaccgct gcagtatcgt
     3721 tggcggaaaa gacaccatca ggcctgtctt tttctttcat tttcaagatg cgttctcctg
     3781 cctgggtgcc ggcatcttca ctaagcttgc tgatatataa tagcttttct tcgaacggta
     3841 ggttgtggtc cctgagagcc tgtttatatc ctttaattct ctccgaataa acattcctca
     3901 gcacattacc tccaagatgc atgattcttc tacaaccctt gtctatcaag tgtttcgtga
     3961 tgtcatatgc tgcttttgca ttatcaataa tgattgaggt gctttcatgg tgcgggaagg
     4021 tccggtcaaa gaaaaccacg ggaattcctt ttttgaaaaa gggtttcagg tgatcaatat
     4081 tttcggtgtc gtaggccagg gagaccagca ggccatccac tcttttatta aataatgtag
     4141 ctgcattggc tacttctttt tctgaacttt ccagggaatg actgatgatc aggttgtacc
     4201 cttctttact ggcaacgtct tccattccgg ccagtacgga agaaacaaaa tagctgttta
     4261 atctggggac tataactcca agagtttgag ttcttttgct gcgcaggctg ctggcaaaag
     4321 tattggaacg gtaccccatt tcctttgccg tatcgtttat ttttttccgg gtatgtttat
     4381 taatcgaggg gtggtctttt aaccccctgc ttaccgttgt agcggaaata ttaagctgct
     4441 tggcaatatc ataaatggta atttctttat cgctcgccat agtgttccct tgtctggtaa
     4501 aggaacttac taattttcaa aaaaaaaatt gcaatcggtt gcaattttaa aaaaaactat
     4561 tatattcgtt ttgccaacta ctaaaaatat cgattgctga acttaaaaat gttcactgtt
     4621 ttcaactgct gtatgaagaa aattagctca aataagccaa ttgttactgc cagtaccgga
     4681 aggttattca gtgattatcc tgacagctat tttgctactt cctttttaaa tttctacatc
     4741 tgtcctgata tatgtttttg ttctgctgat gcaattcagt acatcagcgg atttgctgct
     4801 ccctatactg aagtttctac aactattcaa ttgccaaaat taagattatg aaaaaaactg
     4861 ccagactgaa aaagctattc tttacgagca ggcaaggggt taaacctctt ttattgattg
     4921 tggtactgtt aatgcaatta ttgacagtat ctcagcaatc ctttgctcaa aatgtaactg
     4981 tacggggaaa gatcaccaaa gatgatggtc agccattgtc gggtgcctct gtggtggtga
     5041 agggaactac aaatggtaca acgagcaatg agcagggtaa cttcctgatc tctgctcccg
     5101 gagacgcaac ccttgtaatt tcttccattg attttacaag ccgggaaatt aaagtaaaca
     5161 accagtcaac aataaatgtg acgctttcct cagcagataa aacactggga gaagttattg
     5221 tggttggtta tggtacccaa cgaaaagaag ctattaccgg ttcggttgct tcgataaatg
     5281 gggataaaat gcgtgaagtt ccgtcaccca atgttacaca agccctgcaa ggaaggttag
     5341 ctggtgtgga aatttcgcaa acttcaaccc ggccgggagc taccatgcaa atacgtatca
     5401 ggggtatacg ttctctgagt gccgataata atccgttaat cgttcttgat ggaattccat
     5461 tcatcggttc attggcagat atcaatccta atgatgtaaa aagtattgaa gtacttaaag
     5521 acgcttctgc aactgctatt tatggttccc gtggcgccaa cggtgtactc ctgataacta
     5581 cagataaagg atccaagaat agaaaacccc ggataaatta tagtacttat gttggttcac
     5641 aatccatttt tgctaaatat cccatgatga acggaccgca atttgttgcg ctacgtgccg
     5701 caagggggca atacgccaac ggtcaggatg aggctaacga tgtaaatacc gactggcagg
     5761 atttatttta caaaacagga attgttacag atcataacgt cagcctttcg ggcggttctg
     5821 aagctggcag ctataatttt gggatgggtt attaccttaa ccagggcatt attcctacac
     5881 agcaatacaa gcgttattca ttacgtggat ctattgatca gcaggtgggt aagtattttc
     5941 gatttgggtt taccactaat aataactaca atgaaagcca gggaaaccag gtaggcttat
     6001 ataattcatt aagcatgtcg ccaatttcta ctccgtataa tccagatggt agtatgaaaa
     6061 gaataatacg gatggcagct gatgaccagt tcatttttac caaggatgta gtggagcaat
     6121 taaaagataa cgatcagtgg cttaatgaag atcgtggttt tgcatcttac aacgctatat
     6181 atggtgaagt gaaagcacca tttctcgacg gtttgaaata cagggtaaat cttggtctgg
     6241 attatgtaca attcaataat ggtgcttata caggacaggg tgttggcagc gttaatcctg
     6301 ccagtatatc atctgcttca gtagataaca ggtttactta tcattggaca ttggagaacc
     6361 tgctaactta cgaccacact tttggcggaa aacacacggt gaacgcagtg gccctttatt
     6421 ctgctgagca aaacaaatac aacaggtcaa atatgtcggc acaggatata ccagccgatg
     6481 cttttcaatt ttataacctg ggtcaggctg ccggacaact ggtaataaac ccggccaacc
     6541 aggactacca gttatgggga ttgaagtcag cgatgggtcg tgttatgtac tcttatgaca
     6601 accgctatat gatctctgct actgtgcgtt ctgatggttc ttcaaggctg gcacccggta
     6661 agaaatggca tacttatcct gccttatctg taggatggaa tatcgctaaa gaatcattta
     6721 tgcagaatat tactgccatt aacactttga aactacgtgt aggctttggt caaacttcca
     6781 accaggcgat tgcaccttat tctacacttg gaagattaga taccaggcct tataactttg
     6841 gtccgtcaac ctatagcacc gggttctatg tatcacaact gccaaatgtg gatttaggtt
     6901 gggaattttc aaagaccttg aactttggcc ttgatttttc tgcctttaaa aatcgcttgt
     6961 ccggtactat tgagtactat attaccaaaa ccgaagatat tcttttagga cttagtctgc
     7021 cccccacatc tggtgtgagt ggctacactg caaacattgg ttcaacacaa aacaaaggat
     7081 ttgaacttag cctgaacggt acgatcatca acaatgtaaa tggctggacc tgggaagctg
     7141 gtgttaactt ttatacaaac aacaataagc ttgtatcgct tgcttcagga caaacccggg
     7201 atgaggcgaa ttggtggttt gtggggcata atatcaatgc catctatgac tacaaaaaaa
     7261 ttggtctatg gacggatgca aaagatagtg ctgataatta cttgaatatt ttagaaccgg
     7321 gaggaacagc cggaatgatt aaggtgcttt ataccggtga ttacgatgct aatggcagac
     7381 cggtgagagc aataggacct gccgacaggc aaattatgga tgttgacccc gatttccagg
     7441 gtggctttaa tacccgtgta gcttataaag gttttgacct gggtattgtt ggtatttata
     7501 gaagtggcgg catcctcttc agtaccattc atggttcaaa cggctatctc aatttactga
     7561 caggtcgcag aaataatata aacgtggatt actggacacc aactaataca ggtgcaaagt
     7621 atcccaaccc tgcaggcccg attagtaatg ataatcccag gtatgccagc accctgagtt
     7681 attttgatgg ctcctttatg aaaattcgta ccataacact agggtacgac ctaagccgga
     7741 gcctgataaa aaatcccaat attaatatgc ggatgtattt taccgcacaa aacccgtttg
     7801 ttatgttctc gccattccat aaggaatcgg gtctggatcc ggaaacaaat tcgttcggaa
     7861 atgaaaatgc ggcagtaaac ctcagtcaaa atctgcgtcg tatacttact gttgggttta
     7921 acaccccttc aacccgcaac tatattgtag gcgttaattt gacattttaa atcatacgaa
     7981 tatgaaatat atacaaataa aatctgtttt tgtaactgca ctgtttatgc tgttattttc
     8041 tacgggatgt aataaaattc tggatgaaca gccacgcagt atttatgagc ccggtttttt
     8101 taaaaccgaa aaaggggtgc ttggcggtat tacttctcaa tatgcccacc tgcggttcat
     8161 ttttggtcaa ccctattact ataatacttt agaaaccggc accgacgaat atacctgggc
     8221 ccaaagcgct gaccaaaact ttaaggatat ggatcttagc ggcgttggaa acataacagc
     8281 tgctagcagt cgttctgatg ctttatgggg aacagctttt tctaatatca atactgccag
     8341 cggaattatt gagaatgctt ccgcagttgg tatttcggca ccgttgattg ccgaagcaag
     8401 gtttttccgg gcttttgatt acttcctgtt agtacaaaca tttggcggtg tgccattaga
     8461 ccttggtgcg ggggaattga aattcaatac cactccatca agaacatcag ttcgtaatac
     8521 tgtgcctgaa gtttatacaa aagccatatt ccctgatttg ctgacagcaa taaacgattt
     8581 gcctgctaca ggcagagtaa ccgggggcgc cacaaaaaca ttggcacgcc tttacctggc
     8641 gaaagcctat ttaacctatg cctggtggct gcaaaatccg aacaacattc ctacttatcc
     8701 cttaacgaca agaactgatc cggatggtca tgatgcagca tggtatttcc aaaaggctta
     8761 tgatgtatca gtagaggcta ttgacaaccc aggtccattt ggcttagaaa atacttttta
     8821 tgacgtacac gtaggtggta atgaccgtaa taaagaaatg cttttgtatg ctgaccatac
     8881 gcaggaaagt gaattttata atggatctcc actcacaggt tttcaaagtg aactgggtgg
     8941 tggcagaaat tctgctgttt ggatggtaac ctggaattat actgtaatca gaagtgctcc
     9001 gaattcaaat ggtaccggta gtggtgtaag ctctgtacag cgtgaggccg cccagggtct
     9061 gggacgtccg tatacaagga tggctcctcc gatcggcgtt ttcacaaaca catttgctga
     9121 aaaaacactg gattcccgtt acgatggtac atttaatact gttttcaggg gcaactggcc
     9181 taaaggcgga accacaaatg caacgcttta taacgctaat gatttgccgg ttgctccggg
     9241 tgatcccata ctcagcttcc tcgaatatga ttcgcaggct gctggtataa catattcaac
     9301 aggtgcaaat tttcctggaa aatcttatag taatgttggt gccggtatgt tacccggtcg
     9361 tgccgatttt gtgatcgctc ccagtggaat aagcaggatt gtatttccga atctctggaa
     9421 gatgggggta tatcgtaccg gaagtgttgg attgggagag cctaatgctc caagtacccg
     9481 cccctttaaa attgccaaat tctctgagct ttattttact gctgctgaag cagctgtaaa
     9541 aggtgctacc acagtagcag gtaaaagtgc ccgcgacctg attaatgtga ttcgtgcaag
     9601 ggcaggcaag tggaaatttt cgaacaaaga caatgcagca tacatagcag ataatagtgc
     9661 ggcgatgata gcagccactc ctgctacgat tgacattaac tatatcctgg cggaacgttc
     9721 ccgtgaattt ttcggtgaag gacatcgttg gttcgatttg atacgtactc agaagtggaa
     9781 tgagcttgct ggctcctact caatttgtgc cccaacgtat ggtgctcata caccgcaaac
     9841 atttacacgt acaattgagc caaagcatta tatccggcct attccgcagg ctcagcttga
     9901 cgggatggaa atgacaccgg aagaaaaagc tgcttatcaa aatccgaatt atcaatagga
     9961 ttttaaaatg ctaatcatgt agaagtaaat taaaccgggg atgagttcga tccatccccg
    10021 gtttttaaat ttctcaggag gattaactgc agagtataat gttgactttt caattttcac
    10081 tttgaagatg actaatagaa atgaattgaa gattcctgat gtaaacaaaa aaataaccca
    10141 ttcctgcgaa gggtacgtca aaaaatggca actgatagca gtgatccagc caccaggttt
    10201 attttacttc caatgaaagg gaggcatact taggaatggt attggtatta ttcgttgtga
    10261 gaaaagtaat tccggaatat tgtaaataag tgctttataa ccagtttaaa accagtcatc
    10321 attgaaataa taaataaatt atttgcactg gtttcaaaat cacatatgat tattaagaat
    10381 aaatatttta gaggtctgca aaagctaccg gtaatagttc ttattttatt tgtgtcatgt
    10441 tctaagaaag ggtctgataa taacactggc agcagtacac ctgtacttag tgcagaagtt
    10501 acgattctgg ctaatgaaaa taaacaggtt attcaaggat tcggttgtgc tactgttttt
    10561 gcaccgccaa atacctcctc tttaatcaat gaagagtttg acaggctttt cggttccgga
    10621 acaggccagg taggtttaaa ttttctcaga atcagaattg catctgatga tgcatggagg
    10681 gctacagaac ttaatcatgc aaaagctgcc attcaacgcg gtgcaaaagt atttgcgagc
    10741 ccctggagtc cccctgccag aatgaaaacg aataataata tcattggagg aaaactaatt
    10801 ccagacagtg gggcggctta tgcaagatac ctgaatgact ttgctcttta tatggcttcc
    10861 aatggtgccc cgttgtatgc tgtatccgtt caaaatgagc ctgactggga accaaattat
    10921 gaaagctgtg tatggacggc cacagaaatg agggattttc taaaagatca aggaagctta
    10981 gttacagcta cccgtttaat ggcccccgaa ctggttaaca acaaccaaac ttatgtcaat
    11041 acaattttat ctgatgatgc ggctgctgcc aatttagata ttgttggcac ccatctttat
    11101 ggaggaggta ttattgaaaa tcaattggcc agtaataaag ggaaagaagt atggatgacc
    11161 gaacacttag atacaaatgt cacttatact gcaaacctta atacagcaat tgaaatacat
    11221 gaatgcttta ccaaggcaaa ttttaatgct tatatctggt ggtatggaaa gcggttttat
    11281 ggtccgatag gtcaggatgg aatcataacc aagaggggtt atatcatttc gcaatttgcc
    11341 cggtttataa aagagggagc cgttcgttta ggttcatctg caaattccag aactgatgtg
    11401 ttgatatctg cctatagaaa tggaataaag aaagtggtgg tggcgattaa cctgggtgct
    11461 actgatgtaa aacagaaaat tattttccag ggtactgctg tcacttcgtt tattccctat
    11521 ttaactactt cagcaaagaa tgcagaacaa gggagtatta ttgcaatttc agataatagt
    11581 tttacgtata ctattccggg aaaaagtata gtaaccttag tggagcaata aaattgaaag
    11641 ccaaacatta cgtgtaatac tatgtattaa aaatggaaaa gaattgggga tagaaagagt
    11701 ggaggttacc ggctaagtta ggtatgatgg gttttcagta aataagtttt ttagggtgtg
    11761 ttgtctaaat tttgagaagt ttgaaggtta aagtagttaa aaatgatttg tatcaatatt
    11821 tttttaataa gcagtcgtat agcaatcaaa aaggaggggt gaattctttc atccttcctt
    11881 atttttaaaa ttcaaaaaat ttcaacgatt ggcagcgctt ttactaggaa tagatatcgg
    11941 gacatcgtca gtcaaagcat ctgttgtgga cgcatccacc aggcaatgta ttgctgctgc
    12001 acaataccct gaaacagaat cggacattat tgcactcgaa caaggctggg cagaacagtc
    12061 tgcagatatg tggtgggaac atgtgcaact ggccattctc aagttgcatg cacaacgccg
    12121 gtataatcca aaagatattg ctgctattgg aattgcctac cagatgcacg gcctggtgat
    12181 ggtcgataag gatcaaaaag ttttgcgcaa cagtattatt tggtgcgaca gccgggctgt
    12241 agaaatcggg gacaaagcat ttgaagagat tggccgtgaa aaatgtttgc tgcacctgct
    12301 gaattctccg ggtaatttca ctgcttcaaa actggcatgg gtaaaaacta atgaaccgga
    12361 actatttgcc cgggtagata aaataatgtt gcccggtgac tttattgcca tgaaacttac
    12421 cggggagatt actaccagtg tatcttcatt atcagagggt gtgttttggg attttaagca
    12481 gaacgaatta tcgaaagatg tttttgaata ctatggtttt agtactccca taattcctcc
    12541 tgtcagggat gtttttgcag aacatggaaa agtgttgaag aaagttgctg ttgctttgtc
    12601 tttgactgag gggataccgg ttacttataa atccggggac cagcctaata atgcattatc
    12661 attaaatgta ctggaacccg gggaagtagc ggctacggcc ggtacttcag gagttatata
    12721 tggagtcagt gatcagctga cttatgataa tgaatcaaga gtgaacacct ttgcacatgt
    12781 caattatacc actgctcaaa aaaggctggg ggtgctgctt tgtattaatg gtacgggtat
    12841 tttaaaccgt tgggttaaaa acatagccgg gaactctttg tcttaccagc aaattaatga
    12901 tattgcagca tcagttaagc cgggaagtga tggcatgata atattacctt ttggcaacgg
    12961 agccgaacga atgctgaaga ataaaattgt ccagtctcat atctataata tcgacctgaa
    13021 caagcataac caggcacatc tgtaccgcgc agcgcaggaa ggaatcgctt ttgctttccg
    13081 ttatggactg gatatcatgc gggaaaacgg aatgcatccg caagtgatca gggctggtaa
    13141 agccaatatg tttttaagtg atgttttttc cgaatgcttt gtcaatattc ttgacataca
    13201 agttgagtta tataatagtg atggcagtgt tggtgcagca attgggagcg gattgggcgc
    13261 aaaaatatat accgatgcca aagaagcttt ccggcatttt gaacctattc agctgattac
    13321 ccctaatggt aaaagggcat atgatgaatt ttataataaa tggttgatcc agttagaaaa
    13381 acaattaaac tgatttaata attttaaata ggtaataaaa tgacagtcat aacaggtacg
    13441 aaagaatatt ttaaagggat aagtaagata aaatatgaag gtcctcagtc agataatcca
    13501 ttagcctacc ggtggtatga tgaaagtaaa gtggttgcgg gcaaaactat gaaagagtat
    13561 ttgcggtttg cctgtgctta ctggcacagc ttctgtggga acggtgctga tccattcgga
    13621 gaacctactc atttgttttc atggaatgaa aaaacagatg ctgttgaaag agcaaaagat
    13681 aaagctgatg cagcttttga attcatcaca aaactgggtt tgccatatta ttgctttcat
    13741 gatgtggatg cagtggacta tacaaacgat gtaaaagaaa atgaccgcag actgcaagcc
    13801 ataactgatt atctggcgga gaagcaaaaa gtaagtggtg taaaactact ctggggcact
    13861 gcaaatttat tttccaacag gagatacatg aatggtgcag caacaaaccc cgattttcat
    13921 gtattaactc atggggcagc gcaggtgaag gcagcattgg atgccaccat tgcattgggt
    13981 ggtgaaaatt atgtgttttg gggaggaaga gagggttata tgagtttact gaatacccaa
    14041 atgaaaagag agaaagagca cttcgctgtt tttttacatg cagcaaaaga ttacgccagg
    14101 aagaacgggt tcaaaggaaa attctttatt gagcccaaac cttgtgaacc atccaagcat
    14161 caatacgatt atgacagcga aactgtgatc ggtttcttac ggcaacatga tctgctgaat
    14221 gattttagtt tgaatattga agtaaatcat gcaacactgg ccgggcatac cttcacacat
    14281 gaactacagg tggctgctga tgctggtgta ctgggtagta ttgatgccaa ccgcggtgat
    14341 taccagaatg gttgggatac agaccaattc ccgaatgata ttaatgaatt gacagaagct
    14401 atgctggtta ttctggaagc aggtggtttt aaaggaggtg gaatcaactt cgatgccaag
    14461 atccgtcgca attcaactga cccggaagac ctgtttcatg cacacatcgg cggtatggat
    14521 atttttgcaa gagcattgat cactgctgat aacatactga ataagtccga ttataaaaag
    14581 atcagggcag aacgatatgc atcgtttgac agcggtaaag gaaaggaatt tgaacaagga
    14641 aaattatccc ttgaagacct gagggcttat gcaattgaaa aaggggagcc tgctgttaag
    14701 agtggtaagc aggagtattt ggaaaacatc atcaatagat acatttaata ccgtttaaaa
    14761 ttatctcatg gaaagatttg caacactcga ttgggtagtg atagcctgct attttgccct
    14821 tctgataggc gttgctatct gggtagtaac ccgtaagcaa aaaaatacag aggactattt
    14881 tttagccggc cgcagtttgg ggtggtttgt gatcggcgca tccatctttg cctccaatat
    14941 tggatcagag catgtagtgg gtcttgccgg tgctggtgcc ggaggaaaga tgccattgct
    15001 catttatgaa ttacatgcct ggttggttat tacccttgga tgggtattcc tgccttttta
    15061 cttacgtagt ggtgtattta ccatgcccga atttttagag aaaagattta attccggtac
    15121 ccgatggttt ttaagtgtgt tttcactgat ggcttatatt ttgactaaag tttctgttac
    15181 cgtttatgcc ggtggtattg taatttcttc attgttacat attgatttct ggaccggtgc
    15241 cctggtgacg gtagtgataa caggcgtcta tactgtattg ggaggtatga gggccgtagc
    15301 gtatacagag gcaatccaga ctgtgatact tgtagcaggt gcagccactc taacatttat
    15361 tggcattgat gcagcagggg gctgggcgga agtaaaagca aatcttgccc cgggctatct
    15421 taatatgtgg agacctaaca gtgatgctga gtttccttgg ttgccattgg tcattaccag
    15481 ttcaatcgta ggaacctggt attggtgtac agatcagtat atagtgcaaa gggtattggc
    15541 ggctaagaat attaaggagg ggagaagagg tactattttt ggtggtttat tgaaactttt
    15601 accggtattt cttttcttga ttcctggtgt agtagccctg gcattaaaaa ataaaggtga
    15661 gttaagctgg gatagtgccg accaggcatt tgcttcttta ctgatgaata aaatgcctgc
    15721 tggtttgcgg ggcctggtag ctgccggttt attggcagca ttgatgagtt cgctggcctc
    15781 tgtttttaat tcctgttcaa ctttgtttac cgtagatatt tataaaaaac ttaggcctaa
    15841 tgattcggaa gaaaaattat tgaagatagg ccgaatggct acaacagtgg tagtaatact
    15901 gggcatttta tggataccaa ttatgcagaa tatttcaggc gtattatatg aatacctgca
    15961 atcggtacag gcatatattg caccgccgat cacagcggtt ttcttacttg gaatttttag
    16021 taaacgaatc aacagccagg gagctattac tacactgata gctggtttaa ttatagcaat
    16081 tctcaggatt gcattggaat taagccgcaa ttcactggaa ccgggaagta tgctctatag
    16141 tattgcaact actaattttc tcacttttgc agcgtggttc ttcctgttct gtgtgggttt
    16201 atgtgtaact gtaagcctgc tgacaaaagc accaaccccg gaaaaggtaa tggggcttac
    16261 ctacagtacg ttatctatgg aacaaaaaac agcaaatcgt gcaagctata atgtatggga
    16321 tatcattctt tcattagcgg tcatcgccat cgttgctttt gtaatgataa gttttaccgg
    16381 ttaattcggc agggagagta ttggtacaac ttttattgga tatcgttgat aaaaagcaat
    16441 aagatggact ctttaataca agcaggcctg tcactatttt ttactattaa aaagaaggat
    16501 agttatgtta atggaacaaa ctatgcgttg gttcggacca aacgacccgg ttagcttatc
    16561 tgatatccgg caatcggggt gcactggtgt tgtcaccgca ctacatcata ttcctgttgg
    16621 tgaagtatgg gctatagaag agattgctaa aaggaaacag gtaatcaaag atgcaggtat
    16681 gagctggacg gtgattgaga gcctgccggt aagtgaggat ataaaaaaac agtctggtaa
    16741 ttatttacag catattgaaa attataaaca gagccttcgc aatgtggcgc aatgcggctt
    16801 gaaagtcgtt acctataatt tcatgccggt actggattgg atgcggactg atatttctta
    16861 tatgatgccg gatgggagca gggcattata ttttgagcgt tccgctttta ttgcctttga
    16921 tttattctta ttaaaaagac ccggtgctga acatgattac acggaagagg aaataaaaaa
    16981 agccagattc aggtttgaac ggatgacaga tgaagaagaa gaaactctct tccgcaatgc
    17041 tttgttggga ttgcccggta gcgaggaaca gtttaccgaa gaacagattc tgaaagcatt
    17101 aaagacttat gaagacatag atgcagcaaa gctgaaagca cacctgtatt attttctgca
    17161 acagattgta cccgtagcag aggaatgcgg actcaaaatg gctattcatc cggatgatcc
    17221 gccttatgcc gttctgggtt taccaagaat tgtaagtaca gagcaggatg ctgctgattt
    17281 actgcaggcg gcaccatctc ctgccaatgg attatgtttc tgcactggtt cctttggtgc
    17341 gagaccggat aatgacctgt tgaaaatgct gaaacgtttt ggagatcata tccattttct
    17401 tcatttaaga aatacaaaac gggatgctga aggtaatttt tatgaggcgg atcacctggc
    17461 aggtgattca gatatgtatg aaataataaa ggaagtatta ctgataatgc aacgaagaaa
    17521 tatttctatc cccatgcgac ccgatcatgg gcaccagatg ttagatgatc tgaagaaaac
    17581 aacctatccc ggctactcgg ctattggacg actaaaagga ctggctgaat taaggggtgt
    17641 ggaatttggt attttcaggt cactaaaaag ctaagtcatc ctgattgagt atttgttatc
    17701 gttattacaa attggttaat aattaaaaga cactttatac ctctttttgt ttagtcatta
    17761 ataatgagta ttaacacagt ctcgtaattc ttcaattgac tactaagtgg atttacttaa
    17821 aagcaattct ttttcccgta tttattaact aagcatcaat gttttatgca cattgcattg
    17881 attttttact taaattttct atgtaaaaca ccttatcaat tcaatgcaaa ccactatccg
    17941 tatggggaaa atcaccatcc ttttagttga cgaccataag ctaatcaggg attcatggtc
    18001 ttttattctt aacagcgaca gtcgtttcca ggttattgga gaaaccagca atgcagatga
    18061 agcagttgaa atttcaaagg ataaaaagcc tgaaatagtg ctgatggata tcaacatgac
    18121 cccggtcaat ggatttgaag caacaaagct ggtccggaaa tattcccccg gctcaaaagt
    18181 tattgggata tcaatgcatt ccatgccggc ctatgcacgg cgtatgcttc aactaggggc
    18241 aatgggttat gttacaaaga attcttcaaa ggacgaatta ttaactgcaa ttgtggaagt
    18301 acataatggg aaaaaatata tatgtgatga agtaaaaaac atccttgctc agcaggaatt
    18361 ggaagaagag ggtggtcctc ccgatatgaa tgtgctctcc cgaagggaaa tagaaattgt
    18421 gcagcttatt aaagaaggat tatcatcaaa agaaattgca ttgcgtcttg atatctctct
    18481 taaaacggtg gaagtacacc ggtataacat ccttaaaaag ttaagcctga aaaatacagc
    18541 ggcacttgtt aactttatca atgctcatgg actatgagtc agccattcgg ataaattttt
    18601 taccaacaag taagccctta aaccgattct tatgaatcga ttttataccc gtcagcgtat
    18661 cacaataggt gtaataattt cttttacact ggtttctttt tttgcatggt attcctatat
    18721 aaatatgaat aaggcaagga aagaaacgtt gaaagtaaat tcaacccttc agtccctgaa
    18781 atcacttgaa aacctgatgg atgatatgca ggatattgaa acaagcagca ggggttatct
    18841 catttccggc aataaagaat ttcttgaacc ctattaccgt gcattaacta aactgcgaac
    18901 agatacagta gaactttgga atctgcacca gtcttaccct aacagaaaag aagttttcag
    18961 gagtttgctg aacctggtta atgagaagat cgaaattgcc agctattcag aaaaagtagt
    19021 gaatgaaaat agtgatactg ctgcttttca gctggtaaga tccggcagag gtagagaggt
    19081 gatggatagc ataaggcaga tcatttggtc tgttgaaaat gatgaccgga aagtgttgaa
    19141 tacatcgaat gccagcaggg agattgcagc ggcaactact gcaaaattat tccttggtct
    19201 ggccattggt tttattataa taacgacctt acttttttta cagattcggc gtgatttgaa
    19261 acgacgggaa gaatatgaat ccaggatttc gtatttggct ggtctgacag agaaaacaag
    19321 tgatgcgatt atttctattg atacagggct caggataata agctggaata agggtgcaga
    19381 aaacacttat ggctataccc gagaagaagc aatcggaaag aatattgcgg aattgacaca
    19441 gagtgaaatt ttgaaggaat cggattctca gcttcataaa gaaataagcg ttaaaggata
    19501 cgttgagttt gaatcaaaag attatacaaa agagggaaga agtatatttt gccaggtttc
    19561 taccacgcct ttaaagaatg aactgggtga tgttacgggt tatgtttcgg tagtaaagga
    19621 tattacccag cgaaagttag ctgagaaatt aatgcatgaa ttcaatgttg aattggctga
    19681 aaaagtaaaa gaaaaaacag caggtatcaa tagaagtgag gaaaagctaa gacaagtact
    19741 ggaaagtgct gccagtgaat tttatgtaat tgatcttgaa tacaggataa ttctgattag
    19801 taagttggcc gaactaaatc ttgaaaaagc atggggtaag aaagtgatga ccggcgatat
    19861 tgtaattaac gatcttccgg cagagaaatt ggaaattata cgggataatt ataacagggt
    19921 ctttagaggg gaggaaatcg ggtatgaggc tcaggttgca tccgataatg gagagaggtg
    19981 ggtctatgtg aattatgtgc cagtaaaagg ggaatatgga gaaattaccg gcgcatatat
    20041 tgccacaaaa gatatctcag aaaggaaaaa agtagaaaaa gcgctacttg aaagtgagga
    20101 aagataccgg acacttattg agcaggctat cgattgtatc gtagtaacag attctgatgg
    20161 aaagtttatc caggtaaatg atgccggtat taaaatgttt ggctatacac gggaggaatt
    20221 cctgcaatat tatctgaaag atttgttagt gataggagcc ggggatccac cgatcaggtt
    20281 tgaagagctt cgaagtggta ttggaatttt aagctatcgt aaagccaggc ggaaagacgg
    20341 gacaattttt gatttggaga tcagttcaaa aatgctcccc aatggaaaaa ttattggtat
    20401 tgccagggat atttctgacc gaatgaatgc ccaaagggtg ctggcagaaa gtgaaaacag
    20461 gtttaaggcc atttttaaca cccaatatca aatggttgga ttgcttgata ataatggaaa
    20521 tgtactggaa gtaaataaaa ctactttaga tatcacaggc tatgggaatg ctgatatgca
    20581 gggcaaacct ttttgggaga taagattttg gcaaaatgaa agggatagag cagagaggac
    20641 gggaagggtg aaagaggcaa tacaaaaagc tgcatccgga caatttgtga gatatgaatc
    20701 ggcgatacaa ctcccgggta aagaaattga gataattgat ttttctataa aacctattcc
    20761 tgacgcggat ggaaatatta agcttttaat tattgaagga agattaatta ctgaaattaa
    20821 gaaggcagaa aatgaactca gggaaagcga agccaaatac agggcatttt ttgcgaatag
    20881 cctggatgga attctgctta cctcgccaga tggtactatt ttatct
//
LOCUS       DAORTI010000053        20683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2186_length_20683_cov_7.544697, whole genome shotgun sequence.
ACCESSION   DAORTI010000053 DAORTI010000000
VERSION     DAORTI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20683
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_20683_cov_7.544697"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLO70_08560"
     CDS             complement(<1..756)
                     /locus_tag="PLO70_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQZ74556.1"
                     /translation="MNIQEYISSGITESYVLGLASDEERREFEKMCAQYPEVLQARIA
                     FELALEKQALENAINPTTELKDRIFERIGSTGKIVPLQDTSIKNTQPGALLWLKYAAA
                     ACVVLLAGSLYMNITLSNKNKKLKGSYDNTLAELNEVKKDIRVLQQNPNIKMASMKGM
                     DISPKSYATVYWDTASHDVYLLANNLPVPPNDKQYQLWALLDGKPIDLGMLNYDLKQK
                     KLLVRMKNAQGAEAFAITLENKGGNPTPQGAIYV"
     gene            complement(761..1300)
                     /locus_tag="PLO70_08565"
     CDS             complement(761..1300)
                     /locus_tag="PLO70_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ74557.1"
                     /translation="MNLNTTYSEQELVALLQQRNEKAFSYLYDNYSGALYGIVNAVVT
                     DKEVANDVLQNVFINIWRKIESYDASKGRLFTWMLNIARNAAIDELRSKGYNNALKNI
                     SLPEINEIEGAVTGPAIDDVGLKKVIGKLKGELRVLVDMSYFQGFTHEEIARSLDIPL
                     GTVKTRIRSALTQLRTMIQ"
     gene            complement(1376..1753)
                     /gene="gcvH"
                     /locus_tag="PLO70_08570"
     CDS             complement(1376..1753)
                     /gene="gcvH"
                     /locus_tag="PLO70_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQZ74558.1"
                     /translation="MNFPANLRYTKDHEWISLEGNIATIGITDYAQHELGDIVYVEVD
                     TIGKTLEAGAVFGTVEAVKTVSDLFLPVSGTITELNPALNNAPELVNSDPYGEGWMVK
                     MTVTNPADVEALMNASAYEAVVG"
     gene            1946..4408
                     /locus_tag="PLO70_08575"
     CDS             1946..4408
                     /locus_tag="PLO70_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HQZ74559.1"
                     /translation="MRKLVVFFLGLSMISSTIQAQKVTGLVKDELGKGLEKTTVSLLR
                     AKDSSVVKLAVTGSDGRFVLQSAAGNFLVSVSHIGYTSQYSASFEVNGDINLPEILLS
                     KASTNLQGVTITAQKPMIEVRADKTILNVEGTINAVGNDALELLRKSPSVMVDKDDNI
                     SLAGKNGVQVFIDGKPSPLSGTDLSNYLKSLQSAQIEAIEIITNPSAKYEAAGNAGII
                     NIKLKKNKSFGANGSVNLGYVQGTYPKYNGGVNLNYRNKSINVFGNYNYNNAQYLMSM
                     NSDREQFDTSFSQKNRMLFHNNTHGFKAGLDYFINNKSTLGAVVNGNLANNDFNTSGP
                     MYFTYIPTGQVDRILKATNNNDMKRDNANANLNYRYAVTGGTELNIDADYGIFKIRSN
                     QYQPNYYFQPDGTTEISRAIYNMIAPTDIDLFSLKADYEKNYKGGRLGIGGKIGIVNT
                     DNDFQRYDVYTSGKVLDTLKSNRFKYKENINALYVNYNKQLKKGIMIQFGLRAENTHS
                     KGTSTGFKQVNGTMLTYDSTFKRDYTDVFPSAAVTFNKNPMKQWTFSYSRRIDRPAYQ
                     DLNPFEFKLNEYTFMKGNTLLRPQYTNSFDITHIYKYRLTTKLTYSHVKDIFAQIPDT
                     IDKTKGFMTKRNLATQDVIALNISYPFQYKWYSFFASLNSNYSHYVADFGGGDRKVDQ
                     SVFSLTYFMQNSFNLGKGWTGELSGLFISPSVWQGLIRSEAMGSVDAGVQKVIFKGKG
                     NIKVAVSDIFQTMKWGGYTNFAGVYGQFNGQGEMPQFKLNFSYRFGNSQVKAARQRKS
                     ALEEERKRAEGGGQQGGMGNQK"
     gene            complement(4466..5161)
                     /locus_tag="PLO70_08580"
     CDS             complement(4466..5161)
                     /locus_tag="PLO70_08580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQZ74560.1"
                     /translation="MNKIVGLLLLILLTNTLLAQKDSVVTRKDRKRDVLLQTNYGDIT
                     IRLSDSTPLHRDNFLKLTKTHFYDSVLFHRVIKNFMIQGGDPDSKNAAAGKPLGNGGP
                     AYRIPAEFRQTLFHRKGVIAAARDNNPEKASSGSQFYITQGRIFTDAGLDSVETYRLK
                     RKIPANHREVYKTAGGVPHLDQNYTVFGEVVKGLDVVDKIADVKTSKGADKDRPIENV
                     TIIKAKLIKRKKY"
     gene            complement(5179..5523)
                     /locus_tag="PLO70_08585"
     CDS             complement(5179..5523)
                     /locus_tag="PLO70_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74561.1"
                     /translation="MFSNDDLQQRWWNLEAKLVERFGKKPDLETILFLIGIQEFGNIR
                     DKFTKEQKQDLMHIAVCSLLSASGYYEMESVDQDGWPHFKQLKAMPDMNAIEQENFIK
                     DHVLLYFEDHEL"
     gene            5591..6862
                     /gene="serS"
                     /locus_tag="PLO70_08590"
     CDS             5591..6862
                     /gene="serS"
                     /locus_tag="PLO70_08590"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQZ74562.1"
                     /translation="MLQLQVLRQNPGMVKERLSVKNFSETGLVDTILLLDEQRKKLQL
                     EFDNTQAKVNSISKEIGGLMAKGQKEEAEARKLEVGALKSSLQPISDKLNEVEKQLQN
                     ELVKLPNLPSDKVPAGKTPADNVVVKEGGRKPELHAEAVPHWELIKKYDIVDFETGAK
                     ITGSGFPLYKGKGAKLQRALIQYFLDFNTAAGYTEYLPPLMVNELSAYSTGQLPDKEG
                     QMYYMQVDNFYMIPTAEVPVTNIYRDEIVKESDLPIKMTAYSPCFRREAGSFGADVRG
                     LNRVHQFDKVEIVQIAHPDKSYDVHLEMVEHVEKLLISLELPYRILALCGGDMSFASA
                     LTYDFEVYSAAQQKWLEVSSVSNFESFQANRMKVRFKDEKGKPQLAHTLNGSSLALPR
                     IVAALLENNQTAEEIKLPTVLQSYFGAATIS"
     gene            complement(6943..7911)
                     /locus_tag="PLO70_08595"
     CDS             complement(6943..7911)
                     /locus_tag="PLO70_08595"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HQZ74563.1"
                     /translation="MKKSLVLLAGGFLFLMGCNKSVSNEEGLPEEDVVATQRHCAADE
                     VLKEDLKADPSLRKRMEDIEEFTKRVAANPEQFRLVNGVIEIPVVFNVLYRTAAQNVS
                     LAQLQSQIDVLNEDFAATNADYNLTSTYNSVKSGNIGVRFVLDAVVRKSTNTTSWSTN
                     NAMKKSPKGIAPTSPTTKLNIWVCNMGGGILGYAQFPGGSSATDGVVLDDNATGRTGT
                     AAAPFNKGRTATHEVGHWMNLRHIWGDATCGTDQVGDTPTHNTANYGCPAAGHLSTCS
                     GTPVEMTMNYMDYSDDACMYMFSLGQKSRMLAVFASGGPRNSFAQP"
     gene            complement(8153..9196)
                     /locus_tag="PLO70_08600"
     CDS             complement(8153..9196)
                     /locus_tag="PLO70_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HQZ74564.1"
                     /translation="MSIRAYKYVSPLIIYIGAWHSFNVTGWQIWLPLIWAWIFIPLIE
                     LLLKPSPVNMSAAEEELAKKDRTYDILLYLIVMLQYLLLVRFLIVMSSEGLTLNDSIG
                     RIWVMGLLCGTFGINVGHELGHRVNKLEQTLAKALLLTSLYMHFFTEHNKGHHKRVAT
                     PEDPSSARYGETIYAFYFRTIFFSYLSAWHIANEEMRKKGKSIFSFHNEMLQFHIIQA
                     ILLTTIILIFGWLVTVYFIAAALIGILLLETVNYIEHYGLQRKAIGDGKYERAMPEHS
                     WNSDHIIGRLMLFELSRHSDHHYMASRKYQVLRHHDNSPQMPTGYPGMMILSLFPPIW
                     FYIMNRRIKKLSA"
     gene            9323..10813
                     /locus_tag="PLO70_08605"
     CDS             9323..10813
                     /locus_tag="PLO70_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQZ74565.1"
                     /translation="MSLTNQLKNQHLMWRAGFGPAVEQLSDLPKYTPKQFYKALVKAS
                     EKKPDYIDVADDYLKGLVMGFEEAGRQQKKELDADERKKVQQKNREGVRNLNMYWMHE
                     MVNTASQLREKMAFFWHGHFACRNLNVFYQQGLLDVIRRNALGNFGTMLKEVSKSAAM
                     LNFLNNQQNRKDHPNENFAREVMELFTLGRGNYSENDVKEAARAFTGWGANIKGEFQF
                     RRFQHDFGSKTVLGRTGSFDGGEVLDILLEEKQTAKYITQKIYKFFVNEKADTDKINW
                     LADRFYKNEYDISKLLEDIFTSDWFYDEQNIGAQIKSPVELLVGIQRMLPMKLENEEA
                     LMLLQRVLGQLLFYPPNVAGWPGGKNWIDSSSLMMRMRIPKLINDVDEMNVKAKDDDD
                     TMMGMKDGNVLLKGTKKPGEQQSGGYAGMRRQQIDANVDWRPYVKHYESVPRENLADT
                     ISKILLQTKSRVSMDIIKQFSDQTGRENFIKTATLQIMSTPEYQLC"
     gene            10857..12056
                     /locus_tag="PLO70_08610"
     CDS             10857..12056
                     /locus_tag="PLO70_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQZ74566.1"
                     /translation="MLFKRKEFIQIGSLATASMMLPKFLKAFEGKSMVLPGNKVVVIL
                     QLSGGNDGLNTVIPFRNDLYYKARPKLGIVKDKVLSVTDEVGLHPALAGFKELYDDGS
                     LAILNNVGYPNPDRSHFRSMDIWHTASQSSEYWTNGWVGRYLDAQCNGCDKPTQAIEI
                     DDVLSLALKGENIKGIAVKDPRRLYGTANEKFFRDVMKHHKDEAGEQPVDYLYKTMAE
                     TLSSADYIFQQSRMHPTNAVYPKTDLGNSLKTIASLIYSEINTKVYYVSLGSFDTHIN
                     QDAQQQRLFTEMNDAVKAFVKDLKQQHRFDDVMLFTFSEFGRRVEQNASGGTDHGTAN
                     NMFLVSGGLKQKGILNAMPDLKDLNEGDLKYNVDFKNVYATVLKKWLNADDKSILTKQ
                     YDYLNFI"
     gene            complement(12126..>13349)
                     /locus_tag="PLO70_08615"
     CDS             complement(12126..>13349)
                     /locus_tag="PLO70_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HQZ74567.1"
                     /translation="WDPVAQNFDNWSDAIYKAYIRQIGAGAVAAGGFITLIKTIPTII
                     SSFKGSIGSLKKGGTNTDVQVPRTERDLSMKVVGIGSLILLLVIALLPNTLIPGTNIG
                     SKLLLGLLVIIFGAFFVTVASRIVGLIGSSNSPISGMTIATLMGTCLIFIAVKWTGYV
                     YEPMALVVGGMICIAAANAGATSQDLKTGYLVGATPKYQQISLFVGAIVSSIAIGATI
                     KVLDQPTAEMVSQGIQHAIGTDKYPAPQGTLMATLIKGILSFNLDWQFVLVGVFIAVV
                     MELCGIKALSFAIGIYLPLSTTLPIFIGGAIRGLVEWRQKKKNIQVAPEEEDLGKGNL
                     FATGLVAGGALAGVLVAILSSIESVNTKLGKVNAEHSITNSLGAEGYKWLGVAFFALM
                     GFVLYRIATGKKKTV"
     assembly_gap    13350..13449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13450..13991)
                     /locus_tag="PLO70_08620"
     CDS             complement(<13450..13991)
                     /locus_tag="PLO70_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HQZ74568.1"
                     /translation="MAEQQNHFKPFVSAETKMAELTIKSIITGAIFGIIFGASTVYLA
                     LKAGLTVSASIPIAVIAITLGRRFLKTTILENNIIQTTGSAGESIAAGVVFTLPGFLF
                     LSDPNSANYFNYFTILILAIFGGILGTLMMIPLRRSLIVQEHKTLPYPEGTACASVLK
                     AGEKGGDFAKTAFMGLGFAFA"
     gene            complement(14118..15749)
                     /locus_tag="PLO70_08625"
     CDS             complement(14118..15749)
                     /locus_tag="PLO70_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQZ74569.1"
                     /translation="MWNKHPKALPFLFFSEMWERFGYYLMIGIFTLYLKDVKTGYAMT
                     EAESADLYGTFIALVFLTPFIGGLLADRYLGYRRSVIMGGLMMGVGYCLMSVHNLPML
                     YLSMTLVILGNGFFKPNISTLLGNVYTTPQYILQKDDGYNIFYMGINIGAFICNFFGA
                     ALYIVYGWGAAFIAAGVGMFLGVFIFLAGTKHYKAYDVKKGVTENDMSMTRIVMTIFL
                     PSIVAGIAGWLIPGTLVGSDSTDAFIFACLPVIYFYGSLYFSAKQEEKRPIAALLAIF
                     AVVIMFWAVFKQNGSALNTWADRYTNREVTGMTQQLFDNLNFAQTITYKQDSVAKYDE
                     QFRLMKENGKVVKELNYPVYFKNIPADQKPAEGASVSLWATNLSQSINPGWVILLTPL
                     VVAFFTWLRRRKKEPSTATKIAFGLFISGLSALVMVGAVYASHNGTEKASVLWLISSY
                     GVITIGELLLSPMGLSLVSKLSPVRITSLMMGGWFLATSIGNKLSGVLSTMWDNYNHK
                     AGFFWLNFGLLMVATFMCFAMLKWLNRIMKEKGVS"
     gene            complement(15849..17549)
                     /locus_tag="PLO70_08630"
     CDS             complement(15849..17549)
                     /locus_tag="PLO70_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQZ74570.1"
                     /translation="MSQSQEVFPAQPAHPGRHPKALYALFFTEMWERFAYYLMVGILF
                     IYMTDSTTGGKGFSGRIGADIVGSFIALVYLTPFIGGLIADRYLGYIKSIFIGGSLMA
                     IGYLGLSLPGDTAMFVSLAFIIVGNGFFKPNISTLLGNIYNREDLKPLKDNAYNIFYM
                     GINIGAFVCNFVAAWLRNAYGWGYAFGAAGVGLIIGLIILATAMKDKDIKQADIKKPV
                     QAEDMPLSKIFSTVFLPAIITAVIGWFLPTTIFGSPIMGTQANDAFIFACLPIIAFYI
                     SLWVRAKGQDKKGIGALLFIFAIAIAFWTIYNQNATGLTLWAEKHTDREASASTAKIT
                     GAIFPLQKVTDTPRLVNKLDQYMVEVKDDSGKVVQEMGPDPYFVNVPKEQWPGGKDVK
                     LTNTELFQSINPLFIVLLTLLFVPFFSWLRKRGKEPTTMSKFGMALFISGLSALAMVF
                     AIMSVPSIYTHKASAIWLWITYFIFTISEIFLSPMGLSLVSKLSPARLTALMMGGWFL
                     STSIGGKVAGVMTSFWDDFTDKKIFFLILVAAAFIGGILIYSRIKSLNQIVKDKTGAD
                     "
     gene            17700..18980
                     /gene="purD"
                     /locus_tag="PLO70_08635"
     CDS             17700..18980
                     /gene="purD"
                     /locus_tag="PLO70_08635"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQZ74571.1"
                     /translation="MRILLLGSGGRENALAWKLSQSPRCESLFIAPGNAGTSQFGTNL
                     NFSVTDFESIKKACIDNKIDMVVVGPEEPLVKGVTDFLISSSSLKNLDVIGPGKAGAQ
                     LEGSKAFAKAFMMRHNIPTAAYREFTLDNYTAGIEYLKNHSLPIVLKADGLAAGKGVV
                     ICENHLEAIAEFELMIQRAKFGEAGKKVVVEQFLKGIEISVFVLTDGKNYVLLPEAKD
                     YKRAGQGDTGPNTGGMGAVSPVPFVDEVLMKKIEDKVIRPTVEGLKKEGLEYRGFIFF
                     GFMIVDDEPYMIEYNCRMGDPETEVVIPRLKNDLVELLDAVSNQELDKVKIETDPRTV
                     CTVVAVSGGYPGDYTKGLEINGLGDINPEDSMLFHMGTTIKDGKVVTNGGRVFCITSY
                     GRSIFDAVEISKEEMQKVTFKGMAYRNDIGYEFE"
     gene            19054..20049
                     /locus_tag="PLO70_08640"
     CDS             19054..20049
                     /locus_tag="PLO70_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HQZ74572.1"
                     /translation="MEVNYHDYLQLEKILNAQAPESDKHNLPAHDEMLFIVIHQAYEL
                     WFKQLHHEVDSVGTILSKPALNDNSPALQTIVHRLSRCVTILRVLVHQIDIMETMTPM
                     DFLDFRDMLRPASGFQSWQFKELEAKLGLKFEHRHGQQYYTSQLRKEHVEVIKNAEAG
                     KSLLQLLNSWLERMPFLKEGADESFWSNYKTSYQSSLAEAERNNIEAFEEMFGDKGNA
                     EGRSLSPSACRAALFIMLYRGYPLLQLPFQLLNCLLEIDEQLSSWRYRHVNMVHRMIG
                     TRIGTGGSTGKDYLKAAADKHYIFREIAQLNSFLIERRKLPALSTTVEARLGFNH"
     gene            complement(20105..20572)
                     /locus_tag="PLO70_08645"
     CDS             complement(20105..20572)
                     /locus_tag="PLO70_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74573.1"
                     /translation="MRKIIVSSLALLIFYSSQAQSKSANSSSYQTALGVKVWDGGGIS
                     LKHFINGNNVLEGIAYFWRQGIRITGLYEIHGPISGATGLQWYIGPGAHLGFYNTRNG
                     DGSFIGLDGVLGLDYKFKGAPINMSIDWQPSFEFGDNRGFVGSWGGLGVRYTF"
BASE COUNT         6396 a         4037 c         4265 g         5885 t
ORIGIN      
        1 cacatagata gcaccctggg gagtgggatt gccaccttta ttttcaaggg tgatggcaaa
       61 cgcttcggcc ccttgtgcat tcttcatgcg gaccagtaat ttcttttgct tcagatcgta
      121 attcagcata ccaaggtcaa ttggttttcc atccagtagt gcccatagct ggtattgctt
      181 atcattgggt ggtacaggca gattgttggc caacagataa acatcatgcg aagctgtgtc
      241 ccaataaact gtagcatatg attttggtga aatatccatg cccttcatag aggccatttt
      301 aatatttgga ttttgctgca aaaccctgat atcctttttc acttcgttca attcggccaa
      361 tgtattgtca tagctgcctt tcagcttctt atttttattg gataaagtaa tgttcatgta
      421 caagcttccg gcaagtaata ctacacaggc ggcggctgca tatttcagcc acaacaaagc
      481 tcccggttgt gtatttttaa tggaagtatc ctgcaaggga acaatttttc cggttgagcc
      541 aattctctca aaaattctgt cttttaattc ggttgtaggg ttgatcgcat tttcaagggc
      601 ttgtttttcc aatgccagtt caaaagcgat ccttgcctgt aagacttcgg ggtattgcgc
      661 acacattttc tcaaactccc ttctttcttc atcagaagca agcccgagaa catagctctc
      721 tgtaattcca cttgatatgt attcctgtat gttcactttt ttattgaatc attgttctca
      781 actgtgttaa tgcacttcgt atccttgttt ttactgttcc caatggtata tctaatgacc
      841 tggcaatttc ttcatgtgta aaaccctgaa agtaggacat gtctaccaaa acccttaact
      901 caccttttaa ctttccaatt actttcttta aacccacatc atcaatagcc ggcccagtta
      961 ctgccccttc aatttcatta atttcaggca gcgatatatt cttcagtgcg ttgttgtatc
     1021 ctttggacct caattcatct atagctgcat tacgggctat attcaacatc caggtaaaaa
     1081 gtctgccttt tgaagcgtca tacgactcaa tttttctcca gatatttata aatacgtttt
     1141 gcaatacatc attagcgact tctttatctg taacaaccgc attcacaatg ccatataatg
     1201 caccagaata gttatcatac aaataactga aagctttttc attgcgttgc tgcaacaaag
     1261 ctaccagctc ctgttcgctg tatgtagtgt tgaggttcaa atacttggtt aagacacaat
     1321 tattacaata ataaatgcgt ccgattaaat gaacgaagta aaagtcatta tagatttatc
     1381 cgaccactgc ttcatacgct gaggcattca taagtgcctc aacatcagcc gggttggtta
     1441 ctgtcatttt taccatccag ccttctccat aagggtcact gtttaccagt tccggggcat
     1501 tgttcagggc aggattaagt tcagtaatcg ttccgctaac ggggagaaaa agatcagata
     1561 ctgtttttac tgcttcaaca gttccaaaaa cagcaccggc ttcaagcgtt tttccaatag
     1621 tgtccacttc tacatagact atatcaccca attcgtgttg ggcataatcg gtaatgccaa
     1681 tagtggcaat attgccttca aggctaatcc attcatgatc ttttgtgtaa cggagattgg
     1741 cagggaaatt cataatcaat gattttgagt tgcaaaccta agggaaaaca gtgataaaca
     1801 ttatgaagct ggtaaagctc tttttcagtt tcacccgaat attcatgccg ttcatggcat
     1861 cttttacacc attggtcggg aatagccttt acaatcgcat gacacaagat tatctttgcc
     1921 aaccattaat caaaaacctt taagaatgag aaagttagtt gtattttttc ttggattgag
     1981 catgatatct tctaccatcc aggcgcagaa agtgaccgga ttggtaaaag atgaactagg
     2041 aaaagggctg gaaaaaacaa ccgtgtcatt gcttcgtgct aaagattctt cagtagtaaa
     2101 gctcgccgtt accggatcgg acggacgttt tgtattacag tcggctgcgg gtaatttcct
     2161 ggttagtgta tctcatattg gatacacctc acaatactca gcttcctttg aagtaaacgg
     2221 ggatattaat cttcctgaaa tattactttc aaaagcttcc actaatctgc agggagtaac
     2281 aattactgca caaaagccga tgatcgaggt cagggcagat aaaactattt tgaatgtgga
     2341 gggtaccatt aacgctgtcg gcaatgatgc gctggaattg ctgcggaaaa gccccagtgt
     2401 gatggtggat aaggatgaca atatcagcct ggcgggcaaa aacggagtac aggtatttat
     2461 tgacggaaaa ccgtcaccat tatcaggtac tgatttatcc aattatttga aaagccttca
     2521 gtctgcacag atagaggcca ttgagatcat tactaatcct tctgccaaat atgaagcagc
     2581 aggtaatgca ggcattatta atataaaact gaaaaaaaat aaatcatttg gtgccaatgg
     2641 ttcggtaaac ctgggatatg tgcagggtac ttaccccaag tataatgggg gggtaaacct
     2701 taattaccgc aataaaagca ttaatgtatt tggtaactat aattacaata atgcccaata
     2761 cctgatgtca atgaactctg acagggagca gtttgatacg tcattttctc aaaagaacag
     2821 gatgcttttt cataataata cacatggttt taaagccggg cttgactatt tcatcaataa
     2881 taaaagcaca ttgggtgcgg tcgtaaatgg taacctggct aacaatgatt ttaatacatc
     2941 gggaccaatg tattttacct atatcccaac cgggcaggtg gaccggattt tgaaagctac
     3001 taacaataac gatatgaaaa gggataatgc gaatgctaac ctgaattacc gctatgcagt
     3061 aaccggtggt acagagctga atattgatgc tgattatggc atctttaaga taagatctaa
     3121 ccaatatcaa cccaattatt actttcagcc tgatgggact accgaaatta gccgggctat
     3181 ttacaatatg attgcaccga ctgatattga tttgttttca ttaaaagcgg attatgaaaa
     3241 aaattataaa ggcggcagac tgggtatagg cggaaaaata ggtattgtaa atactgataa
     3301 tgattttcaa cgctatgatg tgtacacatc cggtaaggtg ctggatacgc ttaaaagcaa
     3361 tcgttttaaa tacaaagaga atattaatgc cttatatgta aattataata agcagctgaa
     3421 aaaggggata atgatccagt ttggtttgcg ggccgagaac acccattcaa aaggaacttc
     3481 aaccggtttc aaacaggtaa atggtacaat gttaacatat gattcaactt ttaaaagaga
     3541 ttataccgat gtgtttccca gtgcggcagt tacattcaat aagaatccaa tgaaacagtg
     3601 gacattctct tatagccgtc gtattgaccg cccggcttac caggatttga acccttttga
     3661 gttcaagttg aatgaatata cttttatgaa aggtaatacc ttgctgaggc cccagtatac
     3721 caatagtttt gatataacac atatctataa atacaggtta acaacaaagc tgacctacag
     3781 tcatgtgaaa gatatctttg cacagatacc agacacaatt gataagacga aaggatttat
     3841 gacaaaaagg aacctggcta cgcaggatgt gatagcactg aatatcagtt atccttttca
     3901 gtataaatgg tacagtttct ttgcttcgct caattcaaac tattcacatt atgtagccga
     3961 ctttggtggt ggcgacagga aagttgacca gtcagttttt tcactgacat attttatgca
     4021 aaatagtttc aatcttggca aaggctggac cggggaatta agcggcctgt ttatatcacc
     4081 atcggtatgg cagggcctga tcaggtctga agcaatgggt agcgtggatg caggagttca
     4141 aaaagtaata tttaagggaa agggtaatat caaagtggct gtaagtgata ttttccagac
     4201 gatgaaatgg gggggatata ccaattttgc cggtgtttac ggtcaattta atggccaggg
     4261 tgaaatgcca cagtttaagc taaacttcag ttatcgattt ggtaatagcc aggtaaaagc
     4321 tgcccgccag agaaagtcgg cattggaaga ggaaaggaaa agggcagagg ggggtggcca
     4381 gcaagggggg atgggcaacc agaaataata ttgaaccagt gaatataaaa actcccggta
     4441 aattttaccg ggagttttta ttgtattaat atttttttct tttgatcagc tttgccttaa
     4501 tgattgtcac attttcaatg ggccggtctt tgtcagctcc tttgctcgtt tttacgtccg
     4561 caattttatc cactacatcc aaacctttta ctacctcacc aaatacagta tagttctgat
     4621 ccaggtgagg cacaccgcct gctgttttat acacttctct gtggttggca ggaatttttc
     4681 ttttcagcct gtaagtttca acagaatcta aaccagcatc tgtaaagatc cttccctgcg
     4741 ttatataaaa ctggctgcca ctactggctt tttcaggatt attatccctt gcggctgcaa
     4801 tcactccctt tctgtgaaat aaagtttgcc tgaattctgc tggtattctg tacgcaggcc
     4861 cgccattacc aaggggttta ccggcggctg cgtttttgct atccgggtca ccgccctgga
     4921 tcataaaatt ctttatcacc cggtgaaaaa gtacactgtc ataaaaatgg gtttttgtga
     4981 gcttgagaaa attatcccgg tgaagtggag tagaatcaga aagtcttatt gtaatatctc
     5041 cgtagttcgt ttgtaataat acatctcttt tcctgtcttt tctggtgaca acggaatcct
     5101 tttgcgccaa caatgtattg gtcagtaaga taagaagaag caaacccact attttattca
     5161 tagtgcttaa ttatgttttt atagttcatg gtcttcgaaa taaagtaata catggtcttt
     5221 tatgaaattt tcctgctcga tcgcattcat atccggcatt gcttttaact gtttaaaatg
     5281 tggccagcca tcctggtcta cagactccat ttcataatat ccacttgcag acaacaggct
     5341 gcatacagct atatgcataa ggtcctgttt ttgttctttg gtgaatttat ccctgatatt
     5401 gccaaactcc tggataccga tcaggaataa aatggtttcc aggtctggtt tcttaccaaa
     5461 acgctccacc agttttgctt cgaggttcca ccatcgttgt tgcagatcat cattgctaaa
     5521 catagttcgc aaagataagg tgagcggcac ttgtagcttc tcctttatct ttgcgcaaaa
     5581 ttgaattaag atgttgcagt tacaggtatt aaggcagaat cccggaatgg tgaaagaaag
     5641 attgtctgta aaaaattttt ctgaaacggg attagtggat acgatccttt tgttagatga
     5701 acaacgaaaa aaacttcaac ttgagtttga taatacccag gcaaaagtga acagtatttc
     5761 taaagaaatt ggtggattga tggccaaagg gcaaaaagaa gaagcggaag ccagaaagct
     5821 ggaagtagga gctttgaaat cttctttgca acccatttct gacaagctga atgaagtgga
     5881 gaaacaattg cagaatgaac tggtaaagct tcccaatctt ccttccgata aagtcccggc
     5941 aggtaaaaca cctgctgata acgtggtagt aaaagaggga ggccgtaaac ctgaattgca
     6001 cgctgaagct gttcctcatt gggagttaat taaaaaatat gatatagttg attttgaaac
     6061 aggagcaaag atcaccggaa gcggcttccc tttgtataaa gggaaaggcg ccaaattgca
     6121 acgggcattg atacagtact tcctggattt taatacagct gcgggctata ccgagtatct
     6181 gccgccattg atggtaaatg aattgtcggc atatagcacc gggcagttac cagataaaga
     6241 aggtcagatg tattatatgc aggttgataa tttttatatg attccaactg cggaggtacc
     6301 ggtaaccaat atctataggg atgaaatagt aaaggagtca gatctcccga taaaaatgac
     6361 cgcttattcc ccctgtttta gacgagaggc aggtagtttt ggtgctgatg ttcgtgggtt
     6421 gaacagagta caccagtttg ataaagtaga aattgtgcag atagcgcatc ctgataaaag
     6481 ctatgatgtt catcttgaaa tggtagaaca tgttgaaaaa ttattgatca gcctggaact
     6541 cccttatcgc atattggcat tatgtggtgg agatatgagt tttgcctctg cccttaccta
     6601 tgacttcgaa gtatatagtg ctgcgcagca aaaatggctg gaggtaagct cggtcagcaa
     6661 ttttgaatct ttccaggcta acaggatgaa ggttcgcttt aaagatgaaa aaggtaagcc
     6721 ccagctggcg catacattga atggaagttc tttggcattg ccccgaatag tggcagcctt
     6781 gttggaaaat aaccaaactg cagaagaaat aaaattgccc actgtgttgc aatcttactt
     6841 tggagcagca accatatcct gaaaatttta ctaaactgta tttataaaaa aagtcctgcc
     6901 atggcaggac ttttttttag ttaactaata tagttattaa gattatggtt gcgcaaaact
     6961 atttctgggt ccaccacttg caaacacagc cagcattctt gatttttgac caaggctgaa
     7021 catatacata caagcatcat cagaataatc catatagttc attgtcattt caacaggtgt
     7081 gccggagcag gtacttaaat gaccggctgc aggacagcca tagttagctg tattatgagt
     7141 tggggtatca ccaacctggt cggtaccaca agtggcatct ccccagatat ggcgcaggtt
     7201 catccaatgg ccaacttcat gggtagcagt tcttcctttg ttgaaaggtg cagcagcagt
     7261 tcctgttctt ccggtagcat tgtcatctaa tactacacca tcagtggctg aagaaccacc
     7321 agggaattgt gcgtagccta agataccgcc tcccatatta caaacccaga tattgagttt
     7381 ggtagtaggg ctggtaggag caattccttt aggacttttt ttcatcgcat tattggtact
     7441 ccagcttgtt gtattggttg atttccggac aacagcgtct aatacaaaac gaaccccaat
     7501 attgcccgac ttaacactat tataagttga ggtcaggtta taatcagcat tggttgctgc
     7561 aaaatcttca ttcaatacat caatctgtga ttgtaattgt gcaagagaaa cattttgtgc
     7621 agctgttctg tacaaaacgt tgaatacaac cgggatctca atgaccccgt ttacgaggcg
     7681 gaattgttcc gggttagcag ctacccgttt tgtaaattct tcaatgtcct ccattcgctt
     7741 ccgtaaagaa gggtctgctt ttaaatcttc tttcaatacc tcatcagctg cacaatggcg
     7801 ctgtgtggct actacatcct cttccggcaa accttcttca ttagagacgg atttgttaca
     7861 tcccattaaa aaaaggaagc ctcctgcaag caatacaagt gatttcttca taaattatta
     7921 tttgttttag tgtaggggaa aattgactta gtaatttata agaaattttt agtttccctg
     7981 taatggtcgt tcaattatta ttgcaacagg gtatctaatc ttattcaaac aggaaatttc
     8041 ctgcgtattt actcttattt taaattcatt atttggagat aagctttccc ctatatttat
     8101 cttctttgaa tgtattgaat agcaaggaaa tcaggtgccg cctaattcat tcttaagcgg
     8161 atagtttttt aattcttctg ttcataatat aaaaccagat gggcgggaaa agcgacaata
     8221 tcatcatgcc gggataccct gtaggcattt gtggtgagtt atcatgatgt cttaacacct
     8281 ggtatttacg gctggccata tagtgatggt cggaatgacg gctaagttcg aataacatta
     8341 atcttcctat aatatggtca ctgttccagc tatgctccgg catggccctt tcatatttcc
     8401 cgtcaccaat agcttttcgt tgcaacccgt aatgttcgat ataattgact gtttcaagta
     8461 ataaaatccc aatcagtgca gcagcaataa aatagacagt aaccagccag ccaaatatta
     8521 atatgatggt ggttaggagg atcgcctgta ttatatgaaa ctgtaacatc tcattatgaa
     8581 atgagaaaat tgatttgcct ttctttctca tttcttcatt ggcaatatgc caggcactga
     8641 gataactaaa aaatattgtg cggaagtaaa aggcataaat cgtctcacca taccgggcac
     8701 tgcttggatc ttcaggggtg gctacccttt tatgatgtcc tttattatgt tcagtaaaaa
     8761 agtgcatata gagtgaggta agcaataagg cttttgccaa tgtttgttca agcttattca
     8821 ccctgtgtcc cagttcatgt ccaacattta ttccaaaagt tccacataat agtcccatca
     8881 cccagatgcg acctatacta tcatttagtg tcaatccttc agatgacatg acaattaaaa
     8941 atcttaccag cagcaaatat tgcagcatta ctatcaggta taacagtatg tcataggtgc
     9001 ggtctttttt agccaattct tcctcagcgg cactcatatt aaccgggctt ggtttaagta
     9061 gcagttcaat tagcggaatg aatatccatg cccagatcag cgggagccag atttgccaac
     9121 cggtgacatt gaaggaatgc caggcaccga tataaataat aagcggggag acatatttat
     9181 aagcccggat actcatatta aatttgttag tctttcaaaa ataaaaaata agcttcctta
     9241 tttagcgttt cacttcagat taatatgcat cttttttaaa cttattacct tcataatagt
     9301 ctgaaactta aattatagac caatgtcgtt gacaaatcag ttaaaaaatc agcacctgat
     9361 gtggagagcc ggtttcggcc ctgccgttga acaattaagc gacttgccca aatatacccc
     9421 caaacaattt tataaggctc ttgtaaaagc ctcggagaaa aagcccgatt atattgacgt
     9481 ggctgacgat tacctgaaag gactggtgat gggcttcgaa gaggcaggcc ggcaacaaaa
     9541 gaaggaactc gatgctgacg aaagaaagaa agtacagcaa aagaacaggg agggtgtccg
     9601 caacctgaat atgtactgga tgcatgagat ggtaaatact gcttcacagc taagggaaaa
     9661 aatggctttc ttctggcatg ggcattttgc ctgccggaac ctgaatgtat tttatcaaca
     9721 gggattactt gatgtcattc gaagaaatgc gctgggaaat tttggaacaa tgttaaaaga
     9781 ggtttcaaaa tcagcggcca tgctgaattt cttaaataac cagcaaaacc ggaaagacca
     9841 tcccaacgaa aactttgccc gtgaagtgat ggagttgttt actcttggcc ggggaaacta
     9901 tagtgaaaat gatgtaaagg aagcggcgag agcatttacc ggctgggggg ccaatataaa
     9961 aggtgagttt cagttccgcc ggtttcaaca tgactttggc agtaaaacag tattaggcag
    10021 gaccggaagt tttgacggag gcgaagtact ggatatttta ctggaagaaa aacaaactgc
    10081 aaaatatatt acacagaaga tatataagtt ctttgtaaat gaaaaggcag atacagataa
    10141 aattaactgg ttagctgaca ggttctataa aaatgaatat gatatcagta aactgctgga
    10201 agatattttc acaagtgatt ggttttatga cgaacaaaat atcggagcac aaataaaatc
    10261 accggttgaa ttgctggttg gcatccagcg gatgctaccg atgaaactgg aaaatgaaga
    10321 agcattaatg ttgttacaaa gagtgttagg gcagttatta ttttatcctc cgaatgtagc
    10381 cggatggccg ggaggaaaga actggataga cagctcttct ttaatgatgc ggatgaggat
    10441 acctaaattg ataaacgatg tggacgaaat gaatgtaaaa gccaaagatg atgatgatac
    10501 tatgatgggt atgaaggatg gcaatgtgct gttgaaagga acaaaaaaac caggtgaaca
    10561 acaatccggc ggatatgccg gtatgcgccg tcagcagatt gatgctaatg tggactggcg
    10621 accctatgtg aaacattatg aaagtgtacc cagggagaac ctcgcagata cgatttcaaa
    10681 aatcctgttg caaacaaaaa gccgggtgag tatggatatt attaaacagt tttctgacca
    10741 gactggccgg gagaatttta ttaaaaccgc caccctgcaa attatgagta cgccggaata
    10801 ccaactttgt taaaaccatc tattcactaa cagttaatgg cttaaagatt ttgtatatgt
    10861 tatttaaacg aaaagaattc atccagattg gttcgctggc tacagcatca atgatgctgc
    10921 caaaatttct gaaagcattt gagggaaaaa gtatggtgtt gcccgggaat aaggtagtcg
    10981 taattctcca gcttagtggt ggaaatgatg gattaaacac cgtaattcca ttcagaaatg
    11041 atctgtatta caaagccagg cctaaattag gtattgtaaa agataaagtt ttgtccgtta
    11101 cagatgaagt aggactgcat cctgcattag caggtttcaa agagttgtac gatgatggca
    11161 gccttgctat tttaaataat gtaggttacc caaatcctga ccggtctcat ttcaggagta
    11221 tggatatctg gcacacggcc agccagagca gtgaatactg gaccaatgga tgggttggac
    11281 gttacctgga tgcacaatgt aacgggtgtg ataaacctac acaggcaatt gaaatagatg
    11341 atgtattaag tcttgctttg aaaggggaaa acataaaagg gatagcagtg aaggatccaa
    11401 gacgattata cggaacggct aacgaaaaat tcttccgcga tgtgatgaag catcataaag
    11461 atgaagcagg agaacagccg gtagattatt tgtataaaac aatggctgaa acattatcga
    11521 gtgctgatta tatttttcag caaagccgga tgcacccaac caacgccgtt tatccaaaaa
    11581 ctgatttagg caatagtttg aagacaatcg cttcattgat ttattcagag atcaatacca
    11641 aagtgtatta tgtatcactg ggaagttttg atacccatat caaccaggat gcccagcaac
    11701 agcggttgtt tacagaaatg aacgatgcgg taaaagcttt tgtaaaagac ctgaaacagc
    11761 agcaccgctt tgatgatgtg atgttgttta ctttctcaga gtttggcaga agagtggaac
    11821 aaaatgccag tggtggaact gatcacggca cagccaataa tatgttcctg gtgagtggag
    11881 gattaaaaca aaaaggtatc ctgaatgcaa tgcccgacct gaaggatctt aatgaaggcg
    11941 acctgaaata taatgtggac tttaaaaatg tgtatgctac cgtgctgaag aaatggctga
    12001 atgcagatga caagtcaata ttgactaagc aatatgatta cctgaatttt atttagataa
    12061 agaatccgtg ccctgaaatg aaaaagccca ctcgtctgag tgggctttta attatgtaat
    12121 gaatcttata ctgttttctt ttttccggtt gcgatgcgat aaagtacaaa ccccatcaat
    12181 gcaaagaatg caactcccag ccatttataa ccttctgctc ccaatgaatt tgttatacta
    12241 tgttcagcat ttactttgcc cagtttagta ttaacacttt caatagaaga aagaatagca
    12301 accaatactc cggccaatgc cccgcctgca actaatcccg tagcaaagag atttcctttg
    12361 ccaagatctt cctcctccgg agctacttgt atatttttct ttttctgtct ccattctact
    12421 aaccctctta tggctccgcc aataaagata ggtagtgtgg tagatagtgg caggtaaata
    12481 ccaatagcaa aactcaacgc tttaatgccg caaagctcca ttacaacagc aataaatact
    12541 cctaccaata caaactgcca gtccagatta aacgaaagta ttcctttgat caaggtagcc
    12601 attaaagttc cctgtggtgc aggatattta tctgtaccaa ttgcatgttg aataccttgt
    12661 gaaaccattt ctgcagtagg ctgatcaaga actttaatag tggcaccgat agctatagaa
    12721 gaaacaatcg cgcccacaaa taaagatatt tgctgatatt ttggcgtggc gccaactaaa
    12781 taacctgttt tcagatcctg tgatgtggca cctgcattgg cggcggctat acagatcata
    12841 cctccgacta ccagcgccat aggttcataa acataccctg tccattttac agcaataaag
    12901 atcaaacagg ttcccatcaa agtggcaatg gtcatgccac taattgggct attggatgaa
    12961 ccgatcagcc cgacaatccg ggaggcaact gttacaaaga aagctccgaa tataataaca
    13021 agcaatccta ataataattt actgccaata tttgtacccg gtataagtgt gttgggtaat
    13081 aaagcaatga ccagtaaaag aatcaggctg ccaatgccaa ctactttcat ggaaagatcc
    13141 ctttcagttc ttggcacctg tacatctgta ttcgttcccc ctttttttaa tgatccgata
    13201 cttcctttga atgaagaaat aatagtggga atagttttaa ttaaagtaat gaaaccacct
    13261 gccgccactg caccggcacc tatttgacgg atatatgctt tataaatagc atctgaccag
    13321 ttatcaaaat tttgagcaac aggatcccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnng caaatgcaaa accaagaccc ataaaagcag tttttgcaaa atcacctcct
    13501 ttttcacctg ctttcagcac agaggcacaa gcggttcctt cggggtaagg taaggttttg
    13561 tgttcttgta cgattaatga cctgcgaagg ggtatcatca tcaatgttcc taaaatgccg
    13621 ccaaaaatgg caagtataag aatcgtgaaa tagttaaaat agttagcact gttcgggtcg
    13681 cttaaaaaca aaaatccggg caatgtaaac acaacacctg ctgcaatact ttcacccgca
    13741 gaacccgttg tctgaatgat attattctcc aggatggtag tctttaaaaa tcttctgccc
    13801 aatgtaatag ctattaccgc aataggaata gaggcagaaa ccgtaagccc tgcttttaat
    13861 gccaggtaaa cagtggaagc gccaaatatg attccaaaaa ttgcaccggt tataattgac
    13921 ttgatggtaa gttccgccat tttcgtctct gcggatacaa acggcttgaa atggttttgt
    13981 tgttcagcca tagcagttgg ggttttgttg atgggaagat atgaaaaaag ctattagctg
    14041 tgagccaata gctttgagac aattaatgaa tattattatt ggcttacagc tgagtgctga
    14101 agccttgctg ttttccttta actaactcct ttttctttca tgatcctgtt caaccatttc
    14161 aacatggcaa aacacataaa ggtagctacc atcagtaaac caaaatttaa ccagaaaaaa
    14221 cctgccttgt gattgtaatt gtcccacata gtagacagaa cgccggataa tttattgcca
    14281 atagatgtgg caaggaacca gccacccatc atcaatgagg taatacgtac cggacttaat
    14341 ttagatacca atgacaaacc catcgggctc agtaatagtt ccccaatagt aatcacacca
    14401 tagctggaga tcagccacag tacgctggct ttttctgttc cgttatgact ggcatataca
    14461 gcacccacca ttacaagagc tgatagtccc gaaatgaaaa gaccaaaagc gatttttgtt
    14521 gcagtagaag gttctttctt tcttctccgt aaccaggtaa agaaggccac taccaacggg
    14581 gttaaaagga ttacccaccc gggattgata gactggctaa ggtttgttgc ccataaactc
    14641 acagaagcac cttcagccgg tttttgatct gctggaatat ttttgaagta taccgggtag
    14701 ttcaattctt ttacgacttt accattttct ttcatcaacc tgaactgttc atcatacttt
    14761 gccacagaat cctgcttgta ggtaatggtt tgtgcaaaat tcaggttgtc aaaaagttgt
    14821 tgtgtcatgc ctgttacttc acggtttgtg tatcgatcgg cccaggtatt aagggctgaa
    14881 ccattttgtt tgaaaacagc ccagaacata atgacaacag caaagatggc cagcaaagca
    14941 gcaataggtc ttttctcttc ctgtttggca ctgaaataaa gtgatccata aaagtagata
    15001 acaggcagac aagcaaatat gaaagcatca gtggaatcgg aacccaccaa tgtgccggga
    15061 atcaaccaac ctgcaatacc tgccacaatg gaaggcaaaa aaatggtcat gacgattcgg
    15121 gtcatggaca tatcattctc ggttacacct ttctttacat catatgcttt ataatgtttt
    15181 gttcctgcca ggaaaataaa aacacccaaa aacataccta caccggcagc aataaatgca
    15241 gcaccccagc cataaactat gtataaagcg gcaccgaaaa agttgcagat gaaagcacca
    15301 atattgatgc ccatataaaa aatattataa ccatcatctt tttgaagtat atattgtgga
    15361 gtggtgtata cattacccag cagggtagag atattgggtt taaaaaaacc attacccaaa
    15421 attactaatg tcatagagag gtataacatt ggcaggttgt gtacggacat caggcaataa
    15481 cccactccca tcattaaccc acccataatt acagacctgc ggtagcctaa ataccggtct
    15541 gccagtaagc caccaataaa gggggtcaga aatacaaggg caatgaatgt accataaagg
    15601 tcagcagatt ctgcttcagt cattgcatac cctgttttta catccttcag gtagagggta
    15661 aaaattccga tcatcaggta ataaccaaat cgttcccaca tttcagagaa aaaaagaaaa
    15721 ggcaatgcct tggggtgttt attccacata gttcaatcgt ttaataggga aagataataa
    15781 aaggatggtt agggaaaata aaaaaaggtg gctttgtggg ccacctttgt atgcacaggt
    15841 tataattttt aatcagcacc agtcttatct ttcacgatct ggttgagtga tttaatcctt
    15901 gaataaatta aaattccccc gataaatgca gcagctacca ggataaggaa gaatattttc
    15961 ttatccgtga aatcatccca gaaactggtc attacgccag ctacttttcc cccaatagaa
    16021 gtggagagaa accatccacc catcattaat gccgtcaatc tggctggtga taatttggat
    16081 accaatgata atcccattgg gctgaggaat atttcactga tagtaaaaat aaaataggtg
    16141 atccaaagcc atatagctga agccttatgt gtataaatag aaggtaccga cattatggcg
    16201 aacaccatcg ctaaagctga taggccggat ataaacaatg ccattccaaa ctttgacatg
    16261 gttgtgggtt cttttccccg ttttcgaagc cagctaaaga aaggcacaaa aagcaaggtt
    16321 agtaatacga tgaataacgg attgatagat tgaaataatt cagtgttggt aagttttaca
    16381 tctttaccac cgggccattg ttctttaggg acatttacga aataggggtc cgggcccatt
    16441 tcctgtacaa ccttcccgct gtcgtcttta acttcaacca tgtattgatc cagtttattg
    16501 accaaacggg gagtatctgt aactttttgt aatggaaaaa ttgcaccagt aatttttgct
    16561 gttgaagccg aagcttcccg gtcggtatgt ttttcagccc ataaagtgag cccggtggca
    16621 ttttgattat aaattgtcca gaaggcaatg gcaatggcaa aaatgaataa taaagcacca
    16681 attcctttct tgtcctgacc tttggcccga acccatagag aaatataaaa agctataatt
    16741 ggtaaacagg caaaaatgaa agcatcgtta gcctgtgttc ccatgattgg cgaaccgaaa
    16801 attgttgttg gtaaaaacca cccaattact gcagttataa tagcgggcag aaaaacagtg
    16861 gaaaatattt ttgacagcgg catatcttct gcctgaaccg gtttttttat atccgcttgt
    16921 tttatgtctt tgtccttcat agctgttgca agaataatta atccaatgat caatcctaca
    16981 cccgctgctc caaaagcata cccccagcca tatgcatttc ttaaccaggc ggctacgaaa
    17041 ttgcaaacga aagcaccaat attaataccc atgtaaaaaa tattgtaagc attatctttt
    17101 aggggtttca ggtcttcccg gttgtaaatg tttccaagca gtgtagagat attgggttta
    17161 aaaaagccat taccaactat tataaatgct aatgaaacaa acatagctgt gtcaccgggc
    17221 agagaaagtc ccaggtagcc aatagccatc aaactgcctc caatgaaaat cgatttaata
    17281 tatcccaggt acctgtcagc aattaaccca cctataaaag gtgtcaggta aaccagcgca
    17341 ataaaactac caacaatatc agcaccgatt ctgcctgaaa aacccttacc gccggtggtg
    17401 gaatcggtca tatagataaa cagaatccct accattaaat aataagcaaa ccgttcccac
    17461 atttctgtga aaaataatgc ataaagtgct ttcggatggc ggcccggatg agcaggctgt
    17521 gcaggaaata cttcttgtga ttggctcata aagctttttt tagtttacaa aatcagaggc
    17581 tataatcaat agaagcaacc aaaatacaga aatttaatca cccgcaaatt ttttcgggct
    17641 atctttagcc aacgtacccg atattcgcaa ataagctaaa atccaaccat atttaaccca
    17701 tgcgaattct tttattgggc tcaggtggca gagaaaatgc cctggcctgg aaattatctc
    17761 aaagtccccg ttgcgagagc ctgtttatag ctcccggaaa tgcaggcacc tctcaattcg
    17821 gcactaatct taattttagt gtcaccgatt ttgaatcaat caaaaaagca tgtatcgata
    17881 ataagattga catggttgtt gtaggtccgg aagaaccatt ggtgaaaggg gtgaccgatt
    17941 ttttgatcag tagctcttcg ttgaaaaatc ttgatgtgat cgggcccggc aaagcaggtg
    18001 cacaactgga aggcagcaaa gcttttgcaa aagcctttat gatgcgccac aatattccaa
    18061 cggctgctta ccgggaattt acactggata attatacagc aggaattgaa tacctgaaga
    18121 atcactcttt gccaatcgta ttaaaagcag atggtttggc agcaggcaag ggggttgtta
    18181 tttgtgaaaa ccacctggag gctattgctg aatttgaatt gatgatacag cgggccaaat
    18241 ttggtgaagc cggaaagaaa gtagtggtgg agcagtttct aaagggaatt gagatctctg
    18301 ttttcgtttt aaccgatggt aaaaactatg tgctattacc agaagcaaaa gattacaaaa
    18361 gagctgggca aggcgatacg ggaccaaata ctggaggaat gggagctgta agcccggtgc
    18421 catttgtcga tgaagtgttg atgaaaaaaa ttgaggataa agttattaga cctaccgtag
    18481 agggattgaa aaaagaggga ttagaataca gggggtttat attttttgga ttcatgattg
    18541 tggacgatga gccttatatg atcgaataca actgtcgtat gggtgaccct gaaacagaag
    18601 tggtgattcc tagattaaaa aatgatctcg tggagttatt agatgcggtc tccaaccagg
    18661 aattggataa agtgaaaatt gaaacagatc cccgcactgt ctgcacggtg gtggctgtga
    18721 gtggtggcta cccgggagac tatacaaagg ggcttgaaat aaatggcctg ggtgatatca
    18781 accctgagga cagcatgctt tttcatatgg gaacaacaat aaaagatggg aaagtggtta
    18841 cgaatggagg tcgtgtcttt tgtatcacct cttatggacg gtcaattttt gatgcggttg
    18901 aaatatcaaa agaggaaatg caaaaagtaa ctttcaaggg catggcctat agaaatgata
    18961 taggatatga gtttgaataa cgtcagtggc tccggctttt attccgctga ataattaaat
    19021 tatttacact tctcaccagc caaaattgcc aatatggaag taaactatca cgattatctg
    19081 cagctggaaa aaattctcaa tgcccaggct cctgaaagtg acaaacataa tttgcctgcg
    19141 catgatgaaa tgctcttcat tgtcatacac caggcttatg aactctggtt caaacaactg
    19201 catcacgaag ttgattctgt aggaacaata ctgagcaaac cggccctgaa tgataattcc
    19261 cctgcgttgc aaactattgt tcaccggctt agtcgttgtg ttacgatctt aagagtgttg
    19321 gttcaccaga ttgatattat ggaaaccatg acaccgatgg acttcctgga tttccgggac
    19381 atgctcaggc ctgcatcagg ttttcaaagt tggcagttca aggagctgga agctaagttg
    19441 ggtttaaaat ttgaacatcg tcatgggcaa cagtactata cttctcaatt acgaaaagaa
    19501 catgtcgagg taataaagaa tgctgaagca gggaagtcct tgcttcagtt actgaacagt
    19561 tggctggaaa gaatgccttt tttaaaggaa ggagctgatg aatcattctg gagcaattat
    19621 aaaacaagct atcagtcaag cctggcagaa gctgaaagaa ataatattga agcttttgaa
    19681 gaaatgtttg gtgacaaagg aaatgctgaa ggtcgttcat tatcgccttc agcctgcagg
    19741 gctgcattat ttattatgct gtatcgtggg tatcccttat tgcaattacc ctttcagtta
    19801 ttgaattgtt tgttggaaat tgatgaacaa ttaagttcat ggcgttaccg ccatgtaaac
    19861 atggttcaca gaatgattgg cacaagaata ggaacaggcg gaagtaccgg taaagattac
    19921 ctgaaagctg ccgctgacaa acattatata ttccgggaaa tagcccagct aaacagtttt
    19981 cttatagaaa gaagaaagtt gccagcttta agtacaacgg tggaagcaag acttggattc
    20041 aaccattaaa ctcaaatggc tgtaaagatt taggtccgtt tcacctgttg tgtagcgata
    20101 aaagttaaaa agtatagcga acgcctaatc ctccccaact gccgacaaat ccgcggttat
    20161 caccaaattc aaaagatggt tgccagtcaa tagacatatt gattggagcg cctttgaatt
    20221 tataatcaag tcctagtacc ccatcaagcc cgataaaact accgtcacca tttcgggtat
    20281 tgtaaaaccc taaatgagca ccgggtccta tataccattg cagacctgta gcaccactga
    20341 tggggccatg tatttcatac aatcctgtta tgcgaatgcc ttgtctccag aaataggcaa
    20401 ttccttccag cacattattg ccattgatga agtgttttaa cgaaatgcct ccgccgtccc
    20461 atacttttac ccctaatgca gtttgatagg aggagctgtt ggcgcttttg ctttgtgctt
    20521 gtgacgagta aaaaataagt aatgcaagac ttgaaacaat aatctttctc ataatttaat
    20581 tttaagtgga gttaacaatt tcaagtccct ccttgccatt actatgccaa aaacaggtaa
    20641 gcaatagaga taaataaata aaaactattt aaccttgata atc
//
LOCUS       DAORTI010000054        20530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2211_length_20530_cov_6.945006, whole genome shotgun sequence.
ACCESSION   DAORTI010000054 DAORTI010000000
VERSION     DAORTI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20530
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2211_length_20530_cov_6.945006"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLO70_08650"
     CDS             complement(<1..960)
                     /locus_tag="PLO70_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74574.1"
                     /translation="MDKGAHFFRCDFQVHTPRDIEWSGTKPVSDADRKAYADNFIIAC
                     RKKGLNSIAITDHHDFAFYKFIRQASLDELDFVGQPISPLERIVVFPGLELTLSNPAS
                     CQAILLLDADFPIEQLNFILTKLSIIPNPDNESSTIPTTPIPTAVVNGLTHLHEILDS
                     VDILKGRYIVLPNVSDGGHGSVLRRGFYEAYITMPSVGGYIDGPIIDREGYLNKINGK
                     DRNYGFKSIGVFQTSDNRKESFEDLGKHTTWVKWAIPTAEALRQACLAKESRLSQELP
                     ELPQVYISKIDVTNSKFLGSFSVEFNQQYSALIGGRGTGKSSIL"
     gene            complement(1203..2570)
                     /locus_tag="PLO70_08655"
     CDS             complement(1203..2570)
                     /locus_tag="PLO70_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ74575.1"
                     /translation="MIKETIGKYRWTICTLLFFATTVNYLDRQVLSLLKPTLEEEFGW
                     SNSDYANIASVFQFTYAISMLFAGRIIDKLGTKRGFAWAIIIWSIGAVLHAIAIPLGE
                     GIATILGWIGIAAVPVSIAGFMLSRAVLGFGESGNFPAAIKATAEYFPKKERSFATGI
                     FNSGANVGAILAPLTVPWIAKHWGWEAAFFIIGAIGFLWLFFWLAFYEKPEKQKRLSA
                     AELEYIHSDTEQSELTASTATAEKVSWFKLLAYKQTWAFTFGKFMTDGVWWFFLFWLP
                     AYLKDQYGMTGTEIMLPLSLLYSMTMFGSIGGGYFPIYFIRKGYQPYDGRMKAMLLIA
                     LIPLVVLAAQPLGSISFWMPVILIGIGASAHQAWSANLFTTVSDMFPKKAVASIVGIG
                     GMAGGIGGVIVTKAGGALFDHYKALGHIETGYTIMFAFCAVAYLIAWGVMKSLVPKYK
                     PITDL"
     gene            complement(2641..3303)
                     /locus_tag="PLO70_08660"
     CDS             complement(2641..3303)
                     /locus_tag="PLO70_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQZ74576.1"
                     /translation="MSKTKQIADAIIAQGMLPLYFNADETVSFEILRAVYKAGVKALE
                     YTNRGDAALMNFKKMVAVRNSEMPGMLLGVGTVKNLQTAKDYLAAGADFLVSPGFVKE
                     VADYAVAHDIFYAPGCMTPSEIIAAENSGISFIKLFPGNMLGPEFLSGIKDIFPKLLF
                     MPTGGVDTTKENIESWFKAGVCAVGMGSKLISKKLMEAKDYATIEKMTKEVLALIATI
                     KK"
     gene            complement(3546..3995)
                     /locus_tag="PLO70_08665"
     CDS             complement(3546..3995)
                     /locus_tag="PLO70_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HQZ74577.1"
                     /translation="MIIDTLQNAPRYFSVHPLFAKAFEFIGQTDLANAPDCKSDITEG
                     LKAIFSNKPGTTAEASVAKFECHNEHIDIQLCINGVETIGWKPREKCVTENGGYNPEK
                     DVQLYHEQPDMYFQLTNGQFAIFFPEDVHAPMIGEGVIKKLVLKVKI"
     gene            complement(4067..5092)
                     /locus_tag="PLO70_08670"
     CDS             complement(4067..5092)
                     /locus_tag="PLO70_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQZ74578.1"
                     /translation="MSKKVVTLGEIMLRLSTPDFKRFVQADSFDVTYGGGEANVAAAL
                     CNYGLNGTFVTKVPNNPIGQSAINHLRRYGVDTQFIARGGERLGIYFLETGASMRASQ
                     VVYDRAHASIADVAIEEFDFDKILDGASWFHTTGITPALSDKAAALTEAALKAAKAKG
                     ITTSIDLNYRKKLWSKEKAREVMTKLCQYVDVCIGNEEDADTTLGFKAAHTDVTKGEL
                     NLDGFKDVFKQMKEKFGFKFIASSLRESHSASDNGWSALVYDGNEFYHTKQYNVRIVD
                     RVGSGDSFASGLIYGLVTGMPMNEAAEFGVAASALKHTIPGDLNHATLSEVKDLVKGD
                     GSGRVQR"
     gene            complement(5245..6075)
                     /gene="kduI"
                     /locus_tag="PLO70_08675"
     CDS             complement(5245..6075)
                     /gene="kduI"
                     /locus_tag="PLO70_08675"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQZ74579.1"
                     /translation="MQIRFQNSPAETKQMTTEQLRENFLVQNLVQDDTIQLVYSHYDR
                     VIIGGVKPVNKTLALPNHPELKADYFLQRRELGIINVGGAGTVVADGKEYAINKMDCI
                     YLGKETREVKFKSRHKKDPAVLYLLSAPAHHKYPIKLMTKEKASPVNLGDASTSNKRT
                     IYKYIHLDGIKSCQLVMGLTVLENGSVWNSVPPHTHTRRMEVYFYFDVPAEHRVFHFM
                     GQPQETRHLVMANHEAVISAPWSMHFGCGTSSYGFIWGMAGENQVFTDMDPAPVADLK
                     "
     gene            complement(6079..7518)
                     /gene="uxaC"
                     /locus_tag="PLO70_08680"
     CDS             complement(6079..7518)
                     /gene="uxaC"
                     /locus_tag="PLO70_08680"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQZ74580.1"
                     /translation="MKKFLDENFLLSNAMAERLYHEYAKDMPIIDYHNHLPPNEVAAD
                     KNFENITRVWLNGDHYKWRAMRTNGVDESYITGDKSDWEKFNAWSATVPYTLRNPLYH
                     WTHLELQRYFGITDILNASTAKKVYDETSALLQTKEYSVRGLLERMKVKLICTTDDPV
                     DNLEYHKQIKKDNWSVKVLPAFRPDKAMNVDDVNTFNNYLTGLEKAADISISTYNDYL
                     AALKKRHDYFAANHCSVSDHGLEEIYAEDYTATEIAGIFAKIRSGGALTVEENRKFKS
                     AMLVTFAEWDWEKGFVQQYHLGALRNNNSRMLKQLGPDTGWDSVGDFSQARALAKFLD
                     RLDRDNKLAKTIIYNLNPADNELFATMIGNFNDGTAAGKIQWGSSWWFLDQKDGMTKQ
                     INALSNMGLLSKFIGMLTDSRSFLSFPRHEYFRRLLCNMFGEEVENGELPNDIEWIGK
                     VIQDICFNNAKNYFNWQQIGEATPAAVKA"
     gene            complement(7778..9415)
                     /locus_tag="PLO70_08685"
     CDS             complement(7778..9415)
                     /locus_tag="PLO70_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HQZ74581.1"
                     /translation="MKHKVLKVHPRDNVIVALTSLSKGETISFEGVDYLLPEEIPAKH
                     KFFMQDMKTGDEVIMYGVLVGKAQMNIAKGSRMSTENIKHAAEPYAYRNAKFEWQAPD
                     VSKFSGRTFNGYHRKDGRVGTANYWLFIPTVFCENRNLDVIREAMHNELGYAVTDKYK
                     QYTHHLLDAYKNGEQLDSVNLSPSSNGHHRPFKNVDGIKFLNHQGGCGGIRQDAAILS
                     KLLAAYADHPNVGGVTVLSLGCQNLQVEDFKNDLKKQNPGFDKPLFIFEQQQSQSEEQ
                     LITEAIRKTFEGLIEINKLERQPATLDKLCIGVKCGGSDGFSGISANPSVGYCSDLVV
                     ALGGKILLAEFPELCGVEQELIDRSVNEPTAQKFIHLMKSYDTIVTNAGSSFSMNPSP
                     GNIKDGLITDAIKSAGAAKKGGTSPVVDVLDYTEPATKPGLSLVCTPGNDVEATTGKA
                     ASGATLILFTTGLGTPTGNPVCPTIKVSTNSNLTKRMNDIIDIDCGPVIEGEKTIQQM
                     GEDILEYCIKAASGEVIPKAVLLNQDDFIPWKRGVSL"
     gene            complement(9412..10944)
                     /locus_tag="PLO70_08690"
     CDS             complement(9412..10944)
                     /locus_tag="PLO70_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HQZ74582.1"
                     /translation="MQLSKNAIRQIEAKQGLDIPDEKIFELPEKVLQFGTGVLLRALP
                     DYFIDKANKQGLFNGRIVVVKSTDSDSSAFDRQDGLYTVCVRGVENGKTVEENIINAS
                     VSRVLSAKTEWKRILECAHNPEMKIVFSNTTEVGIQLVQDDITNEPPVSFPGKLLAFL
                     YERFKAFNGSHESGIVIVPTELITDNGTKLEGIVLELAHRNNLDYKFIEWLENHNTFC
                     NSLVDRIVPGKPNAADSKKIETELGYQDELMTMSEVFRLWVIEGDDKVKEVLSFCKVD
                     KAVIIAPDITLFKELKLRLLNGTHTFNCGTAFLCGFNITREAVLHPVYGAFTKELMHK
                     EIAPAIPFAIDEKVKTDFANSTFERFANPFIDHQWQSITVQFTSKMKMRNMPLLQRHY
                     ELHSTVPMHMATGFAAYLLYMKVTKKAGTKYYGERNSPDGHRDEYEIKDDAAEYFYKV
                     WNNGNAIEAAAEIMANEELWEIDLTKLPGFLQAVQEQLQDMITNGVLATVEQLENKKV
                     TA"
     gene            complement(10947..11987)
                     /locus_tag="PLO70_08695"
     CDS             complement(10947..11987)
                     /locus_tag="PLO70_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQZ74583.1"
                     /translation="MRVRSNTSIFLISVLLMLQACAVAQPSSSVKRITVAADGSGDYK
                     SVQAAINSLPDSSATPRTIYIKKGTYAEKIYIEKANIIFEGEDREKTIIIASIARDEW
                     RCSHTDDWGVATMNIGANDITLKNLTITNNYGFDFTERTIWCASDTSKNKERKLRKDG
                     HQMALRTMNMATKLKAVNCRFRSFGGDTVSPWETMNGMWYFKDCIMEGGVDFYCPRGW
                     AWAENCEFITHTGPAAIWHDGSGNEDSKTVLLNCKFTGYDGFMLGRYHREAQFYLINC
                     AFAKNMKDTPIYRVPTNNVINWGERIYYYNCHREGGNDFKWYSNNLPAAVKAGDITVN
                     WVFGNRWNPEKN"
     gene            complement(11984..15484)
                     /locus_tag="PLO70_08700"
     CDS             complement(11984..15484)
                     /locus_tag="PLO70_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQZ74584.1"
                     /translation="MKKLLFIFSVLAIGISSIAQQGKLPVVISPKFKKDTVDITKFGA
                     IPDGHTLNSKSIKAAIDAISKKGGGVVMIPAGLWLTGPVVLKSNINLHLANGATLLFT
                     RDFNEYPLVEANWEGLPQMRNQSPISATNASNIAITGKGIIDGNGDAWRMVKKDKLNE
                     SQWKKLLASGGVLSDDKKTWYPTEKTLKGSRLSNPGVISPEKDASFYDSIKDFLRPNL
                     LLLTNCKYILLEGVTFQNSPAWCLHPLMSEHLTVRNITVKNPWYAQNGDGIDVESCKN
                     VLIENSVFDVGDDALCIKSGRDAEGRKRGMPTQDVIIRGCTVYSSHGGFVIGSEMSGG
                     AKNIYVSNCTFIGADIGLRFKTTRGRGGVVENIFIKDIYMKDIPGEAILFDMYYAAKD
                     PIPLAGEKRELPKVEFKPVDETTPVFKNFYISNVYCNGAEKGIFVRGLPEMHVKDIVL
                     ENMVLQAHKGIDVQEASGITFRNIKIISEETNPVIDIVHSDKLVFDNITYKENAELLF
                     RINGERSNTINIKNTDASKAKEKIKYELGATEKSIVSVSSAPSPNEKWSERISETAMK
                     LWPDSFILAGDKAAKWRYDQGVILKGIRAVWAATGDGKWFNYIQKSMDFYVQPDGSIK
                     GYRPDEYNIDHINNGKLLLFLFQVTGKEKYKKAVDLLRNQLRTHPRTSEGGFWHKKIY
                     PYQMWLDGLYMGQPFYAEYAKLFHEDSAFNDIASQFILMEKYAYDKKTGLLYHGWDES
                     KAQQWADKTTGQSPNFWGRSLGWYGMALVDVLDHFPANHPKRAELIAILHRFAIAVRK
                     VQDNKTGLWYDVPNMPNEPKNYVEASASSMLAYTFAKGARKGYLPGAYLDHAKQAYRG
                     IIKEFIETDANGQVNLKGTVAVSGLGGKPYRDGSFAYYMSEPVIVNDPKGMGAFIKCA
                     AEMELNETQNIAGGKTVLLDYYFNNEWKKDITGTPIRWHYTWEDKTNSGYAMLGDIFT
                     KYGAKTRSLEKLPTSATLKGASIYIMVDPDTDKETETPRFLNGKDAITISNWVKEGGV
                     LVLLSNDAGNAEFKNFNLLARKFGIQFNEDSKNRVQNDQFEQGVVMTGTGNPVFKSDW
                     KLYVKEYSSLNITAPAVTFVKNGDDNVMAVAKYGKGTVFALGDPWIYNEYLDGRKLPA
                     DFQNYKAAEEWVQWLLKQAK"
     gene            complement(15627..16055)
                     /locus_tag="PLO70_08705"
     CDS             complement(15627..16055)
                     /locus_tag="PLO70_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ74585.1"
                     /translation="MKKLIVSALLIFIVSVAAAQSKQEKAVASAVEKLRLAMIDANKT
                     ELEKLVAEKLSYGHSGGKVEDKNSFVSNITNGNSDFVTIDLTEQSISVSGKTAIVRHI
                     LKAKTNDSGKPGDVQLRIILVWQKQGGQWKLLARQAVKMG"
     gene            complement(16058..17452)
                     /locus_tag="PLO70_08710"
     CDS             complement(16058..17452)
                     /locus_tag="PLO70_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ74586.1"
                     /translation="MERRKFLGYTAAAGTALMLHPFEGFAFSPGKKLKLALVGTGHRG
                     SGFWGSNLLKSFNDVIEFVGLCDINPGRMAYVKQAMNVSCPSFTDFKQMLIATKPDYV
                     IVTTVDAEHDEQIVTALEMGCDVITEKPMTTDEIKCKRILEAEKRTGKKVIVAFNYRH
                     SVHAMQLKELLAQQRIGKVTSVDFNWYLNVYHGADYFRRWHGFVNKGGSLWVHKATHH
                     FDLLNWWLNSEPVEVSAYGALEHYGKNNSFRGTKCRGCEHKDNCKFYWDITKDERLTN
                     LYVKNEQHDGYLRDGCVWRNEIDIWDKMSAQIVYANAVVVNYSLTTYSPYEGWQIAFN
                     GTDGRIETWEDIPYLQKTADDQANRHAMEMSNADDAIPGEFREIMVMDNFKKNYEIFT
                     TPKIKGGHGGGDIRMQRRIFVDKNDNPHNVMAGTREGAMSILIGIAARKSIALKRPVK
                     ITELTDLIPMAKRF"
     gene            complement(17468..19075)
                     /locus_tag="PLO70_08715"
     CDS             complement(17468..19075)
                     /locus_tag="PLO70_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQZ74587.1"
                     /translation="MKKWLTILLVSASFVATAQTVSKVWVADNGKGTYKNPVINADYS
                     DPDAIRVGDDYYMISSSFNHAPGLPILHSKDLVNWTLIGHALRQQLPIEHYSKVQHGA
                     GVWAPSIRYHNNEFYIYYPDPDFGIYLIKAKNILGPWTEPVMVEAGKGLIDPCPIWDN
                     DGKVYLVHAYAGSRAGIKSIIVVKELNKEGTKAISDAVMVYDGHDNDPTIEGPKFYKR
                     NGWYYIFAPAGGVSTGWQTILRSKNIYGPYERKVVIDQGNTSINGPHQGAWVDTKTGE
                     HWFLHFQDKDAYGRVVHLQPMKWINDWPVIGEDKDGDGKGEPVLVFKKPNVGKTYPIT
                     TVADSDEFNSTKLGLQWQWQANPKPYWAFPSNGYLRLFSAQLPNSVKNYWDVPSLLMQ
                     KFPAGEFMVTTKLTFKPRLDGEKTGLIIHGADYAYLSLVKKAGGNYISFSHCSNADKG
                     KEEVHIYGDKINGADIYFRVKVGKGAVCEFSYSEDGTSFKNIGDKLVAKPGRWVSAKV
                     GLFCTRTVKTNDSGYADVDWFRFELVQ"
     gene            complement(19072..>20530)
                     /locus_tag="PLO70_08720"
     CDS             complement(19072..>20530)
                     /locus_tag="PLO70_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQZ74588.1"
                     /translation="IKSAIRWVRKNAATYNIDVNRIIVSGHSAGGELAAFMGATNGMT
                     QFEGNGCYKEYSSKVNAVIDVDGTLAFIHPESGEGDDSKRISAATHWFGYSRTENPDL
                     WKQAAPLTHVGKHTPPTQFINSGVARMHAGREDFVAVLKQHKIYSETKTFEGSPHSFL
                     LFNPWFDTTVVYMDKFLRRVFPAAPKKFAREITVAQDGSGDYKTVQAAINAVPVNNNK
                     PIAIIIKKGIYKEKILIDSLRPFITLIGEDKLNTVLTYNDHTGKLAPNGDTINTRTSW
                     SFKIMANNFTARDITFQNDAGFSAGQAVAVESDGDKAVFINCRFTGNQDVLFTNSERS
                     RQYYEHCYIEGTTDFIFGSSTVWFEKCHIYSKKNSHITAASTPREKEFGYVFNWSRLD
                     GDSSLVNVSLGRPWRPYAAVAYLHCYIGRHIKAEGWSVWNKNDNHLTSRYAEYNNFGP
                     SSDPEARVKWAKHLTAEEAKKYTLLNVLRGWNPLI"
BASE COUNT         5353 a         4753 c         3916 g         6508 t
ORIGIN      
        1 aagtatagaa gatttaccgg ttcctctacc accaattaaa gcactatact gttgattaaa
       61 ttcaaccgag aatgagccga gaaattttga atttgttaca tcaatttttg aaatgtaaac
      121 ctgtggtaat tcaggaagtt cttgacttaa ccgagattcc ttcgctaggc aagcttgtct
      181 taaagcctct gctgtgggaa ttgcccattt aacccaagta gtatgtttac ctaaatcttc
      241 aaaactttct tttctattgt cagaagtctg aaaaacaccg attgatttaa aaccatagtt
      301 tctatcctta ccattaattt tattcagata accttcacgg tctattatgg gaccatcaat
      361 gtaaccacca acagaaggca ttgtaatata tgcttcataa aatccccttc ttaaaacaga
      421 accgtgacca ccatcagaaa catttggaag tacaatgtat cttcctttta gaatatcaac
      481 agagtcaagt atctcgtgta gatgagtcaa tccattaaca acagctgttg gaattggtgt
      541 tgtcggtatt gtggaagatt cgttgtcagg gttgggaata attgatagtt tggtaagtat
      601 aaagtttaat tgctctatag gaaaatctgc atcaagtaaa agaattgctt gacatgaagc
      661 gggatttgaa agtgttagtt ctaagccagg aaatacaact attctttcca atggagaaat
      721 aggttgtcct acaaaatcca attcatcaag tgatgcttgt cttatgaatt tgtaaaatgc
      781 aaaatcgtga tggtcggtta ttgctattga gtttaatcct ttttttctac aggcaataat
      841 aaaattgtct gcataagctt ttctatcagc gtctgaaact ggttttgtcc cactccattc
      901 tatatcccta ggggtgtgta cctgaaaatc gcatctaaaa aaatgtgctc ctttgtccat
      961 agtttacttt atatactgtc ggttcttaaa atggctggta atatgtgtat tatcgaatat
     1021 acgaagtgtt ttttttgaga aactcaaaaa ataatcggtc tgattgtcag tgtcttagat
     1081 tagtttaagc taaattttta acctaaaggt gtttcggcaa tccaaaatcc atttatatcc
     1141 tattaacaaa aaaaaccccg ccatgcgggg atttaaaaag attcaataag taactaaata
     1201 aattatagat ccgtaatcgg tttatacttt ggcaccagtg atttcataac tccccatgca
     1261 ataagataag ccaccgcaca gaaggcaaac atgatggtgt aaccggtttc tatatgtccc
     1321 agtgctttgt aatggtcgaa cagggcacca ccggcttttg ttacgataac accacctata
     1381 ccaccggcca ttccgccaat acccacaata gaagcaacgg cttttttagg aaacatatca
     1441 ctcaccgtag taaagagatt ggcactccaa gcctggtggg cagacgcacc gataccgatc
     1501 aggataacgg gcatccagaa actgatggaa ccaagcggtt gtgcagccag taccaccagc
     1561 gggatcaatg caatcagtaa catggctttc atccggccat cataaggctg gtatcctttc
     1621 cggataaaat aaataggaaa gtaaccgcca ccaatactgc caaacatggt catgctgtag
     1681 agtaatgaca agggcagcat gatctctgta cctgtcatac cgtactgatc tttcaggtaa
     1741 gccggcaacc agaataaaaa gaaccaccac actccatctg tcataaactt gccaaacgta
     1801 aaagcccatg tttgtttata ggccagcaat ttgaaccagg atactttttc tgcggtagca
     1861 gttgaagccg tcaattctga ttgttcagta tcgctatgta tatattcaag ttcagcggca
     1921 gataatcttt tttgtttttc gggtttttca taaaatgcaa gccagaagaa cagccataaa
     1981 aatcctatag ccccgatgat aaagaaagcg gcttcccagc cccagtgttt ggcgatccag
     2041 ggaacagtta atggcgccag tatggcaccc acattggcac cggaattaaa tatacctgtg
     2101 gcaaatgatc tttctttctt tggaaaatat tcagcagttg ctttgatagc agcaggaaag
     2161 ttgcccgatt caccaaaacc gagtactgct ctcgacagca tgaaacctgc aatagataca
     2221 ggcacagcag caataccgat ccagccaagg atggtggcaa tgccttcgcc taaaggaata
     2281 gcgatggcat gcagcacggc acctatggac cagatgatga tggcccatgc aaaacctctt
     2341 tttgtaccca gcttgtcaat gatacggccg gcaaacaaca tggagatggc ataggtaaac
     2401 tggaaaacag aagcgatatt ggcatagtca ctgttgctcc agccaaattc ttcttccagg
     2461 gttggtttca gcaggcttaa tacctggcgg tcgagatagt ttactgtagt ggcaaaaaat
     2521 aacagggtac agatggtcca cctgtatttt ccaatcgttt cttttatcat caggcaatta
     2581 ttaaatccac ttcttctccg gtaaactcaa aattgatccc ggagtaatgc aggggattgt
     2641 ttatttttta atggttgcta ttaaggctaa aacttctttg gtcatctttt caatcgtagc
     2701 gtaatctttt gcttccatca gtttcttgct gatgagtttg ctgcccatgc ccactgcaca
     2761 aacaccggct ttaaaccaac tttcaatgtt ttctttggtg gtgtcaacac caccggtggg
     2821 cataaataat aatttaggga agatatcctt tattccgctt aaaaactccg gtcccagcat
     2881 attgccgggg aacaatttga taaaactgat gccgctgttt tctgcggcga tgatctctga
     2941 aggggtcata caacccggag cataaaatat atcatgggca acggcatagt cggccacttc
     3001 ttttacaaaa cccgggctaa cgaggaagtc tgcaccggca gccaggtagt ctttggccgt
     3061 ttgtaaattt tttacggtac ccacgccaag cagcataccc ggcatctccg aattgcggac
     3121 agccaccatc tttttaaagt tcattaaggc tgcatcgcca cggttggtat attccaatgc
     3181 ttttacacct gccttgtaca ctgctcttaa tatctcaaaa cttactgttt catctgcatt
     3241 gaaatacaaa gggagcattc cctgtgcgat gatagcatct gctatttgtt ttgttttgct
     3301 catatatttt tttttaccac aaagtgctca aagtctatac acaaagaaca caaagcaaat
     3361 tgcgtcttgt attaatggta ccctttgtgt tgttatctta gtgttctctg tggtttaagt
     3421 ttttatttta agcactaatt ttttttatca caaaggtttc aaagtttata cacaaagaac
     3481 acaaagccaa ttgcgtctta tactaatggt accctttgtg ttgttatctt agtgttctct
     3541 gtggtttaaa tttttacttt aagcactaat ttttttatta ccccttcgcc gatcatcggc
     3601 gcatgcacat cttccggaaa gaaaatagca aactgcccgt tggtcagctg gaaatacata
     3661 tcaggctgtt catggtatag ctgtacatct ttttcgggat tgtagccgcc attctctgta
     3721 acacattttt ccctcggttt ccagccaatg gtctccactc cattgatgca aagctgtatg
     3781 tcgatatgct cattatgaca ttcaaacttg gcaacagatg cttcagccgt cgtaccgggt
     3841 ttattggaaa agatggcttt caatccttcc gtaatatctg atttgcaatc cggtgcattg
     3901 gccaggtctg tttgcccgat aaattcaaat gcttttgcaa acagcggatg aactgagaag
     3961 tatcttggtg cattttgcag tgtatctatt atcataacat tattctttac tggattacaa
     4021 acttcaaacc tcaaacttca aacttcaaac ttcaaacctc aaacgcttac cgctgtaccc
     4081 gtccgctgcc atcacctttc accaggtctt ttacttcact taaagtggca tgattcaggt
     4141 ctccgggtat tgtgtgtttt aatgctgagg cagctactcc aaattcagca gcttcattca
     4201 ttggcatacc ggttaccaat ccatagatca ggccactggc aaatgaatcg ccactgccta
     4261 cccggtctac gatacgtaca ttgtattgtt tggtatgata gaattcattg ccatcataga
     4321 ccagggcact ccagccattg tcactggcac tatggctttc ccttaatgaa gaagcaatga
     4381 acttaaaccc gaatttctct ttcatctgtt tgaagacatc tttgaaacca tcgaggttca
     4441 gctctccttt ggtcacatcg gtatgtgctg ctttaaagcc caatgttgta tctgcatctt
     4501 cttcattacc gatacacaca tctacatact ggcagagttt ggtcatcact tccctggctt
     4561 tttctttgct ccatagtttt ttgcggtagt taagatctat gctggtggta atgccttttg
     4621 ctttggccgc tttcagtgct gcttctgtta atgcagccgc tttgtcactt aatgccgggg
     4681 taattcctgt agtgtggaac cagctggcac catcgagtat tttatcaaag tcaaattctt
     4741 caatagcaac atccgcaata gaggcatggg cccggtcgta cactacctgt gaggctctca
     4801 tcgaggcacc tgtttcgaga aaatagatac ccagtctttc gccacccctg gcaataaatt
     4861 gtgtgtccac accataacgg cggagatgat tgatggcgga ctgcccgatg gggttgttgg
     4921 gtactttggt aacaaaagtt ccgttcaaac cataattaca taatgctgct gctacgtttg
     4981 cttcaccgcc accgtaggtc acatcaaaac tatctgcctg tacaaaacgt ttgaaatcgg
     5041 gagttgacaa acgcaacatg atttcaccaa gtgttactac tttctttgac atcgttgttt
     5101 gttttattgt tagttattgt ctgaaagata catcctgaac atttaaaaat ccctgcttta
     5161 ccgggccttt cgctgtattt atttacggta tcgttgccgg tcttttttcg gcatcgttac
     5221 cggtagttca agttgcgggt tttattattt taagtccgct accggggcag gatccatatc
     5281 ggtaaatacc tggttttctc cagccattcc ccatataaaa ccataactgg aagtaccgca
     5341 accaaaatgc atactccatg gtgcagagat cacagcttca tgattggcca tcaccaggtg
     5401 ccttgtttcc tgcggctgtc ccataaaatg aaataccctg tgttctgcgg gcacatcaaa
     5461 ataaaaatac acttccattc tccgggtatg tgtatgtggt ggtacagaat tccaaacact
     5521 accattctcc agtactgtca atcccattac cagctggcaa cttttgattc cgtccaaatg
     5581 gatgtatttg taaatggttc tcttgttgga agtggaagca tcaccgagat ttaccgggga
     5641 agctttttcc ttcgtcatta atttaatcgg gtatttatga tgtgccggtg cagataataa
     5701 gtatagaaca gcaggatctt ttttgtgacg gcttttgaat tttacttccc tggtttcttt
     5761 gccgagataa atacaatcca ttttattgat cgcatattct tttccgtcag ccactactgt
     5821 tcctgcgccg cctacattga tgatacccaa ttcccttctt tgtaaaaaat aatctgcttt
     5881 caattcagga tgattgggaa gtgctaatgt tttattgaca ggctttacac ccccaattat
     5941 taccctgtcg taatgagaat ataccagctg aattgtatcg tcctgcacca ggttctgcac
     6001 caggaaattt tcccggagtt gttctgttgt catttgcttt gtttctgcag ggctgttttg
     6061 aaatcgtatt tgcatggtct aagcttttac agcagcaggg gttgcttctc cgatttgctg
     6121 ccagttgaaa taattctttg cgttgttaaa gcaaatatcc tgtatcactt taccgatcca
     6181 ttctatatcg ttgggcagtt cgccattctc cacttcttca ccaaacatat tgcacaataa
     6241 ccttctgaaa tattcatgac gggggaagga gaggaaacta cggctgtccg tcagcatacc
     6301 aatgaactta ctgagcaatc ccatattgct taaggcattg atctgtttgg tcatgccatc
     6361 tttctggtcg aggaaccacc aggatgatcc ccactgtatc ttgccggcag cagtgccatc
     6421 attgaaatta ccaatcattg tcgcaaacaa ttcgttgtcg gcaggattca gattatagat
     6481 aattgttttc gccagtttat tgtccctgtc cagccggtcc aggaattttg ccagtgccct
     6541 tgcctgtgaa aaatcaccta cactgtccca gcctgtatca ggacccagct gttttaacat
     6601 ccggctgtta ttatttcgca atgcaccgag atggtattgc tgtacaaatc ctttttccca
     6661 atcccactcc gcaaacgtaa ccagcatggc tgatttgaat ttcctgtttt cttctaccgt
     6721 caatgcgccg ccggaacgga tcttggcaaa gatgccggcg atctccgtgg ctgtataatc
     6781 ctctgcatag atctcttcca gtccgtggtc gcttacgctg caatggttgg ccgcaaagta
     6841 atcatggcgt tttttcaggg cagcgaggta atcattatag gtgcttatgg aaatatcagc
     6901 cgctttttcc aaaccagtca ggtaattatt gaaggtgttc acatcatcca cattcatggc
     6961 tttatccggg cgaaacgccg gtaatacttt tacagaccag ttgtcttttt ttatttgctt
     7021 atgatattcc aggttatcaa ccggatcatc ggtggtgcag atcagtttca ccttcatcct
     7081 ttccagcaat cctcttacgc tgtattcttt tgtttgcagc aacgccgaag tttcgtcata
     7141 tactttcttt gctgtacttg catttaagat atcggtaata ccaaaataac gttgcagttc
     7201 caggtgcgtc cagtggtaga gcggatttct taatgtatag ggaacagtag ctgaccaggc
     7261 attgaatttt tcccagtcgc ttttatcgcc agtaatatat gattcatcca ccccgttcgt
     7321 tcgcatggca cgccatttat agtggtcgcc attcagccac acccgggtaa tattctcaaa
     7381 atttttatct gcggccactt cgttgggcgg caggtgattg tgataatcaa taatgggcat
     7441 gtctttggca tactcatgat acaaacgttc tgccattgca tttgacaaca ggaaattttc
     7501 atcaagaaac tttttcataa aattcaattt aaaatattta tttacccgct gcaatgctgc
     7561 tatcagccct gttgcgtcgc actcttcatc gttctgtaat tctattcagc aatattgaac
     7621 ccctccgggg ttcggattac atcgttttat ttgtctccgg gtttcacccg gagctattga
     7681 gattttaccc cttcggggta gaaccacata ttgctattta actaacagct ataacatatg
     7741 actttatgac ctaagtctcc ctttcgggga taggtgttta caaactcacc cctctcttcc
     7801 acggaataaa atcatcctgg ttcaacaaca ctgcttttgg tattacttca ccactggcag
     7861 ctttgatgca atactccagt atatcttctc ccatctgctg aattgttttt tctccttcga
     7921 tcaccgggcc gcagtcaata tctataatgt cattcattct ttttgtgaga ttgctgtttg
     7981 tcgaaacttt aatggttgga caaacaggat taccggttgg cgtacccagg cccgtagtaa
     8041 ataagataag ggtggctcca ctggcagctt tacccgtggt ggcttctaca tcattacctg
     8101 gcgtgcatac caggcttaag cccggctttg tagcaggttc agtataatcc aatacatcta
     8161 caacgggtga agtgccgcct ttttttgccg ccccggcact tttgatggca tccgttatca
     8221 atccatcttt aatattaccc ggtgatgggt tcatcgaaaa actggaaccc gcattcgtaa
     8281 caattgtatc atagcttttc atcaggtgaa tgaatttttg agccgtgggt tcattcaccg
     8341 agcggtcaat caattcctgc tccactccgc atagctcagg aaattctgct aacagaattt
     8401 taccacccag cgccacaaca agatccgagc aataaccaac agagggattg gcagaaatac
     8461 cactgaagcc atcactgccg ccacatttaa caccaataca taatttatca agcgttgcag
     8521 gttgtcgttc cagtttattt atttcaataa gcccctcaaa tgttttgcgg atggcctctg
     8581 ttatcagttg ctcctcactt tgtgattgct gctgttcaaa aatgaacaag ggtttatcga
     8641 aaccgggatt ctgctttttc aaatcattct taaagtcctc tacctgtaaa ttctgacatc
     8701 ccaggcttaa cactgttacg ccacccacat tcggatgatc tgcatacgca gctaataatt
     8761 tgctgagtat ggctgcatct tgcctgattc cgccacatcc gccctggtga tttaaaaatt
     8821 ttattccatc cacattctta aatggacggt gatgaccatt ggaagaaggg gagaggttaa
     8881 cgctgtctaa ctgctctcca ttcttgtatg catccagtaa atggtgggtg tattgtttat
     8941 acttgtccgt cactgcatag cccagctcat tatgcatggc ttcccgaatg acatctaaat
     9001 tcctgttctc acaaaaaaca gtggggatga acaaccagta attagcggtg cccactcttc
     9061 cgtcctttct gtggtagcca ttgaatgttc taccgctgaa tttggaaaca tccggtgcct
     9121 gccattcaaa ttttgcatta cggtatgcat aaggctcagc agcatgtttt atattttctg
     9181 tcgacatgcg gcttcctttt gcaatattca tttgtgcttt tcccactaac acaccataca
     9241 tgatcacttc atcacctgtt ttcatatcct gcataaagaa tttatgcttg gcgggaattt
     9301 cttccggtaa caggtagtca acaccttcaa acgagatcgt ttctcctttt gacaggctgg
     9361 ttaatgcaac aattacattg tccctggggt gtacttttaa aaccttgtgc ttcacgccgt
     9421 aactttttta ttttcgagtt gttcaacagt tgccagcacg ccgttagtta tcatatcctg
     9481 cagttgctcc tgtacggcct gcagaaagcc gggtaatttg gtcagatcaa tctcccacag
     9541 ctcttcattg gccattattt cagcagcggc ttcaatcgca ttgccgttat tccaaacttt
     9601 gtaaaaatac tccgctgcat cgtcttttat ttcgtattca tcccggtgac catcaggact
     9661 atttctttca ccgtaatatt tagtgccggc ttttttggtc actttcatat acagcaggta
     9721 agcggcaaag ccagtggcca tatgcatggg aacagtactg tgtaactcat aatgcctttg
     9781 caacaatggc atattccgca ttttcatttt ggaagtgaat tgaacggtaa tactctgcca
     9841 ctggtggtct ataaagggat tggcgaaacg ttcgaatgtg ctgttggcaa aatcagtttt
     9901 tactttttcg tctatggcaa aaggaatagc cggtgctatt tctttatgca tgagttcttt
     9961 ggtgaaagcc ccgtaaacag gatgcagtac tgcttccctg gtaatattga aaccacagag
    10021 aaaggctgtg ccgcaattga aagtatgtgt tccattcagc aaccgcagct ttaattcttt
    10081 gaacagggta atatccggcg caatgataac tgctttatcc actttgcaaa aagacaacac
    10141 ctcttttact ttatcatccc cttcaatgac ccagagccgg aatacttcgc tcattgtcat
    10201 cagctcatcc tggtagccca gctcagtttc aatttttttg gaatctgctg cattaggttt
    10261 gccgggtacg atacggtcta ccagtgaatt gcagaatgta ttatgattct ccaaccactc
    10321 gatgaattta taatcaaggt tattacgatg ggccagttca agtacaatgc cctccagttt
    10381 ggtgccatta tcagtgatca gttcagttgg cactatcaca attccgcttt catgacttcc
    10441 gttgaatgct ttgaaccgtt cgtacaaaaa ggccaacagc ttgccgggga aggagaccgg
    10501 tggttcatta gtgatatcat cctgcaccag ttgaataccc acttctgttg tattggagaa
    10561 aacgatcttc atttcaggat tatgggcaca ttccaaaatt cttttccatt ctgttttagc
    10621 actcagtacc ctgctgacgg atgcattgat aatattttct tccaccgttt tcccattctc
    10681 cacaccgcgg acacaaaccg tatagagacc atcctgcctg tcgaatgcac tgctgtcact
    10741 gtctgttgat tttaccacca caattcttcc attgaacagg ccttgcttgt tggctttatc
    10801 aataaaataa tccggtaaag ccctgagtaa aacaccagtg ccgaattgca ataccttttc
    10861 aggcaactca aatatttttt catccgggat atctaatccc tgctttgctt caatctgcct
    10921 gatagcattt ttagataatt gcataatcag tttttttcgg gattccagcg gttgccgaaa
    10981 acccagttaa cagttatatc acctgctttc acagcggccg gaagattatt gctgtaccat
    11041 ttgaaatcat ttccgccttc ccggtgacag ttgtaataat atattctttc accccagtta
    11101 attacgttgt tcgttggcac acggtaaata ggagtgtcct tcatattttt tgcgaatgca
    11161 cagttgatga gatagaactg tgcttccctg tggtaacggc cgagcataaa cccatcatag
    11221 ccggtgaact tacaattcag caacaccgtt tttgaatctt cattccctga gccatcatgc
    11281 cagatcgctg cgggccctgt atgtgtaatg aattcacaat tctctgccca ggcccagcca
    11341 cgggggcaat aaaagtcaac gccgccttcc ataatacaat ccttgaaata ccacatgccg
    11401 ttcatggttt cccatgggct cacggtatca ccgccaaaag atctgaaacg acagttcacg
    11461 gctttcaact ttgtcgccat attcatggta cgtaaggcca tctggtgacc atcttttctt
    11521 agtttccttt ctttattttt tgaagtatct gaagcacacc agatcgttct ttctgtaaaa
    11581 tcaaaaccat agttattggt gatagtgagg tttttcagcg taatgtcatt agccccgata
    11641 ttcattgtag caacacccca gtcatctgta tggctgcaac gccattcatc tcttgcaata
    11701 gaagcgataa tgatagtctt ttcccggtct tcaccttcga aaatgatatt tgctttttcg
    11761 atatagatct tttcagcgta ggttcctttc tttatataaa tcgttcttgg ggttgcagat
    11821 gagtcaggca gactgttaat tgcagcctgt accgatttat aatcaccgct gccatccgca
    11881 gccaccgtaa tcctttttac tgaggaagag ggttgcgcca ctgcacaggc ttgtaacatc
    11941 agcagtactg aaattaaaaa gatacttgta tttgatctta ctctcattta gcttgtttta
    12001 acaaccactg cacccattct tctgctgctt tgtaattctg aaaatcggca ggcaattttc
    12061 ttccatctaa atactcgttg tagatccatg gatcaccaag ggcaaacact gttcctttgc
    12121 catactttgc aacagccatt acattgtcat ctccgttctt tacaaaggtc acggctggtg
    12181 ctgttatgtt caggctgctg tattctttca catataattt ccagtcagac ttaaaaacag
    12241 gatttccagt tccggtcatc accactccct gttcaaactg atcgttctgt acccggttct
    12301 tactgtcttc attgaactgt attccaaatt tccgggccag caggttgaag tttttaaatt
    12361 ctgcattgcc agcatcatta ctgaggagta ctaatactcc gccttctttc acccagttgc
    12421 tgatagtaat tgcatctttt ccattcagga agcggggtgt ttccgtttct ttatctgtat
    12481 ccgggtccac catgatataa atagaagctc cctttagtgt agcactggtc ggtaattttt
    12541 ccagtgatct tgtttttgcg ccatacttcg taaaaatatc acccagcatg gcatagccac
    12601 tgttggtttt atcttcccag gtataatgcc agcggatggg tgtgccggtg atgtcttttt
    12661 tccactcatt gttaaagtaa taatcgagta aaacggtttt tcccccggcg atattttgcg
    12721 tttcgttcag ttccatttca gccgcacatt taatgaatgc acccattcct ttggggtcat
    12781 ttactattac cggctcactc atatagtagg caaagctgcc gtcacggtaa ggtttaccac
    12841 cgagacctga cacagcaaca gttcctttga ggtttacctg gccgttggca tcagtttcaa
    12901 taaattcttt aatgataccg cggtatgcct gtttggcatg atccaaataa gcaccgggga
    12961 gataaccctt cctggcgcct ttcgcaaaag tatatgccaa catactggag gcagatgctt
    13021 ctacatagtt cttaggttca ttgggcatat tgggaacgtc ataccataac cctgttttat
    13081 tatcctgcac ttttctgacc gcaatggcaa agcgatgcaa aatggcgatc agttcagctc
    13141 ttttaggatg attggccgga aaatgatcca gcacatctac caatgccatt ccataccagc
    13201 ccaaacttct tccccagaag ttgggtgatt gtcctgttgt tttatcagcc cattgctgtg
    13261 ctttactttc atcccatcca tgatacagca gtcctgtctt tttatcgtaa gcatattttt
    13321 ccatcaggat aaattggctg gctatatcat tgaaggcaga atcttcatga aaaagtttgg
    13381 cgtactcagc gtaaaagggc tgacccatgt acaggccatc cagccacatc tggtacgggt
    13441 atattttttt atgccagaaa ccaccttccg aagtgcgggg atgtgttctt aattgattgc
    13501 gcagcaggtc gaccgctttc ttatattttt ctttcccggt gacctggaac aaaaacaata
    13561 ataattttcc attattgata tggtcaatat tatactcatc gggtcggtat cctttaatgc
    13621 ttccatcagg ctgcacatag aaatccatac ttttctggat atagttaaac cacttgccat
    13681 cacctgtagc agcccataca gcacggatgc ctttgagtat aactccctgg tcgtatctcc
    13741 atttggcagc tttatctcca gccaggataa atgaatcagg ccatagtttc atggctgttt
    13801 ctgagatgcg ttcactccat ttttcatttg gagaaggagc ggagctgaca gaaacgatgc
    13861 ttttttctgt ggcgcccagt tcatacttta ttttttcctt tgctttggag gcatcggtat
    13921 tttttatatt gatagtattg cttctctctc cgttgatacg gaataacagt tctgcatttt
    13981 ctttataagt gatattatca aataccagtt tatcgctgtg cacaatatca ataaccgggt
    14041 ttgtttcttc actgatgatt ttgatgttcc tgaatgtaat gccagaagct tcctgtacat
    14101 caattccttt gtgcgcctgc agcaccatat tttccagcac aatatctttg acatgcattt
    14161 cagggagacc tcttacaaaa attcctttct ctgcaccatt gcaataaaca ttactgatgt
    14221 aaaagttttt gaaaacagga gtggtctcat ccaccggttt gaattccacc ttgggtaatt
    14281 ctcttttttc gcctgccagc ggaatgggat ctttcgctgc ataatacata tcgaacagga
    14341 tcgcttctcc cggaatatct ttcatgtaaa tgtctttgat aaagatattt tccaccacac
    14401 cgccacgtcc tcttgtagtt ttaaaccgaa ggccgatatc agcaccaata aaagtgcaat
    14461 tactgacata aatattttta gcgccaccac tcatttcgct accaattaca aaaccaccat
    14521 gcgaagaata aaccgtacat cccctgataa taacatcctg ggtgggcatt cctctttttc
    14581 taccttctgc atcacgacca ctttttatac aaagggcatc gtcaccaaca tcgaatacag
    14641 aattttcaat caatacattt ttacagctct ctacgtcaat accatcccca ttctgcgcat
    14701 accagggatt ttttacagta atatttctta ctgtcaaatg ttcactcatc aacgggtgca
    14761 ggcaccaggc cggtgagttt tgaaaagtta ctccttccag cagtatgtat ttacagtttg
    14821 taagcagcaa caggtttgga cggagaaaat ctttgatact gtcataaaag gaagcatctt
    14881 tttccgggct gataacaccg ggattggaca gcctggaacc tttcagtgtt ttttcggttg
    14941 ggtaccatgt ttttttatca tcactaagta ctccaccgga agccaacagt tttttccact
    15001 ggctttcgtt aagtttatct ttcttcacca ttctccaggc atcaccattg ccgtcaatga
    15061 ttcctttacc ggtgatggca atattgcttg cattggttgc cgaaatggga gactggttcc
    15121 gcatctgcgg taatccttcc cagttggctt ccaccagcgg gtattcatta aaatctctgg
    15181 tgaacaacaa ggttgcccca tttgctaaat gaagattgat attactttta agaacaaccg
    15241 ggcccgttag ccacagacca gccggaatca ttacaacacc tccccctttt ttggagattg
    15301 catcgattgc agcttttata cttttagaat ttaatgtatg accatcagga atggcaccga
    15361 attttgtaat gtctaccgtg tcttttttaa atttcggaga gataacaaca ggcaattttc
    15421 cctgctgggc aatggaactt atgccgattg caaggacgct aaatatgaat aataattttt
    15481 tcatcaggca aacatgcttt ttacggcgtt tttgttatac aagtctaacc agatattgtc
    15541 tgctaatcaa cgaattgcat tcctttattt acgcaatcgt taccgggaac ggtatcggtt
    15601 tgcgggcagg tttttacaag ttattcttac cccattttca cagcctgcct ggccagtaat
    15661 ttccattggc caccctgctt ttgccaaact aaaataatcc tcaactgaac atcaccgggc
    15721 ttaccactat cattggtttt tgctttcaaa atatggcgga caatagctgt tttaccacta
    15781 acactgatgg actgctcagt aagatcaatt gttacaaaat cagagttccc gttcgtgata
    15841 ttgcttacaa acgaattctt gtcttctact ttgccgccgc tatgaccata gcttaatttt
    15901 tcagcgacta atttttccaa ttcagtttta tttgcatcga tcatcgccaa ccgtagtttt
    15961 tccactgcag aagcaacggc tttttcctgt ttgctttgtg cagcagcaac agacacgata
    16021 aaaatcagca gggcagatac aatgagtttt ttcatactta aaatcgttta gccattggta
    16081 taaggtctgt gagttcagta attttcaccg gtctttttaa tgcaatactt ttcctggccg
    16141 ctataccaat cagtattgac atagcacctt ctcttgttcc tgccattacg ttatgagggt
    16201 tatcattttt gtccacaaag atcctgcgct gcattcttat gtcaccacca ccatgaccac
    16261 cttttatttt cggagtagtg aatatctcat aattcttttt gaagttatcc atcaccataa
    16321 tttccctgaa ttctcccggt atggcatcat cggcattgct catttccatg gcatggcggt
    16381 tggcctggtc atcagctgtt ttttgtaagt atggtatatc ttcccatgtt tctattctgc
    16441 catcggttcc gttaaaggcg atctgccatc cttcataagg agagtaagtg gtgagggaat
    16501 aattgacgac aacagcattg gcatatacta tctgtgcact cattttgtcc cagatatcaa
    16561 tttcatttcg ccacacacaa ccatcccgca ggtaaccatc atgctgttca ttctttacat
    16621 acaggttggt caatctttca tcttttgtga tatcccagta gaatttgcag ttgtctttgt
    16681 gttcacagcc acggcatttg gttccgcgga aagaattatt ctttccatag tgctctaatg
    16741 caccgtaagc acttacttcc accggctcac tgttgagcca ccagtttaat aaatcaaaat
    16801 gatgggtggc tttatgtacc cagagtgaac cgcctttgtt tacaaatcca tgccagcggc
    16861 ggaaataatc agctccgtgg tatacattca ggtaccagtt aaaatcaaca gaagttactt
    16921 tgccgatccg ttgttgtgcc agcaattctt ttaattgcat cgcatgtaca ctgtgccggt
    16981 aattgaaagc aacgatcact ttttttcctg ttctcttttc ggcctccaat attcgtttgc
    17041 attttatttc gtctgtggtc atgggttttt cagtgatcac atcgcaaccc atttccagtg
    17101 ccgttacgat ttgctcatca tgttctgcat ctactgtagt tacaattaca taatccggtt
    17161 tggttgctat cagcatttgt ttgaaatcgg tgaaagaagg acagcttaca ttcatcgcct
    17221 gctttacata ggccattctg ccgggattga tatcgcagag accaacgaat tcaatgacat
    17281 cattaaatga tttcagcaga ttacttcccc agaaaccaga tccgcggtga ccggttccta
    17341 ctaaagccaa ttttagtttc ttacccggcg agaaagcaaa gccttcaaac gggtgcagca
    17401 tgagtgcagt acctgctgcg gcagtatagc ccaaaaattt acgacgttcc atatgcgatc
    17461 gtttatttta ttgtacaagt tcaaaccgga accaatcaac atctgcataa cccgaatcat
    17521 ttgttttaac tgttcgggta caaaacaacc ccacttttgc actgacccag cggcctggtt
    17581 tagccaccag tttatcgccg atatttttaa aactggtacc gtcttcactg tagctgaatt
    17641 cacatacagc tcctttgccc actttcaccc ggaaataaat atcagcaccg tttattttat
    17701 ctccgtagat atgtacttct tctttgcctt tgtcagcatt actgcaatgg ctgaatgaaa
    17761 tataattacc acctgctttt tttaccagcg acagataagc ataatctgct ccatgaatga
    17821 tcaaaccggt cttttctcca tcgagtcttg gtttgaatgt aagctttgtt gttaccatga
    17881 actcgccggc cggaaatttt tgcatcagca ggctggggac atcccagtaa ttttttacgc
    17941 tgttcggtag ctgcgcagaa aataatcgta aatacccatt tgaaggaaat gcccagtatg
    18001 gtttcggatt cgcctgccat tgccattgca atcctaattt ggttgaattg aattcatcac
    18061 tgtctgctac ggttgtaatg ggataagttt taccaacatt tggtttctta aaaactaata
    18121 cgggttcacc ttttccatca ccatctttat cttcaccgat caccggccag tcgtttatcc
    18181 atttcatcgg ttgtaaatgc actactcttc catacgcatc cttatcctgg aaatgcagga
    18241 accaatgctc tccggttttg gtatctaccc atgctccctg gtgcggacca ttgatggaag
    18301 tgttaccctg gtctattacc acctttcttt cataagggcc ataaatattc ttgctcctta
    18361 aaattgtttg ccatcctgtg cttacaccac ctgccggagc aaagatgtaa taccagccat
    18421 tgcgtttata aaacttcggg ccttcaatgg tcgggtcgtt gtcatgacca tcataaacca
    18481 tcactgcatc actgatagct tttgttcctt ccttatttaa ttcttttact acaataatgg
    18541 atttaatacc agcccggctt ccggcatagg catgaacgag ataaactttt ccatcattat
    18601 cccagatagg gcaggggtct atcaatcctt taccggcttc caccatcacc ggctcagtcc
    18661 atgggccaag aatgtttttt gctttgataa ggtaaatacc aaaatcagga tcggggtagt
    18721 aaatatagaa ttcattgttg tgataacgga tgcttggtgc ccatacacca gcaccgtgct
    18781 gtactttgct gtaatgttca attggtaact gttgtcttaa tgcatgaccg atcaaagtcc
    18841 agttcaccag gtcttttgaa tgaagaatgg gcaatcccgg tgcatgatta aaactggaag
    18901 aaatcatata ataatcatcg cccacccgta tcgcatccgg gtcactgtag tcagcattaa
    18961 ttaccggatt cttataagtg cctttgccat tatcagctac ccaaactttt gaaacagttt
    19021 gtgcagtagc aacaaaagat gcacttacta gtaatatggt cagccatttt ttcatatcaa
    19081 cgggttccag cctctcaaaa catttaataa ggtatatttt tttgcttctt cggctgtcag
    19141 atgtttagcc cattttaccc gggcttcggg atctgaggat ggaccgaaat tgttgtattc
    19201 tgcatagcga ctggtcaaat gattatcgtt tttgttccac acactccagc cttctgcttt
    19261 gatatgccgg ccgatataac aatgtaaata agcaacagca gcataaggtc tccacggacg
    19321 gcccaacgat acattaacca gtgatgagtc gccatctaaa cggctccagt taaaaacata
    19381 cccaaactct ttttctctgg gagtagatgc agccgtgata tgggaattct tcttgctgta
    19441 aatatgacat ttctcgaacc acaccgttga agaaccaaaa ataaaatcag tagttccttc
    19501 aatataacag tgctcatagt attgacggct tctttcgcta ttggtaaata agacatcctg
    19561 gttgccggta aaccggcaat tgataaaaac agctttatca ccatcagatt cgacagctac
    19621 agcctgccct gctgaaaatc ccgcatcatt ctgaaatgta atatctctag ccgtgaaatt
    19681 gttggccatt attttaaaac tccaggatgt cctggtattg atcgtatctc cattgggagc
    19741 aagttttcct gtatgatcat tataggtaag aaccgtatta agtttgtctt cgccaattag
    19801 agtaatgaat ggccggagtg aatcgatcag gatcttctct ttgtagattc cctttttaat
    19861 aataatggca ataggtttat tattgttaac aggcacagca tttattgcag cctgcaccgt
    19921 tttataatct ccgctaccat cctgcgctac tgtaatttcc ctggcaaact tttttggggc
    19981 tgccggaaaa acccggcgaa gaaatttatc catgtaaact acggttgtgt caaaccatgg
    20041 attgaataaa agaaatgaat gcggagaacc ttcaaatgtt tttgtttctg aataaatttt
    20101 atgttgcttt aatacagcaa caaaatcttc tcttcctgca tgcattcttg ctacaccact
    20161 attgatgaat tgagttggcg gcgtatgttt tcccacatga gtcaacggcg ctgcctgctt
    20221 ccacaagtcc gggttttctg ttctggaata tccaaaccag tgtgtagcag cagagattct
    20281 tttactgtca tcaccttcac ctgattccgg atgtataaaa gccagtgttc catctacatc
    20341 aattactgca ttgactttac ttgaatattc tttgtaacag ccgttccctt caaattgtgt
    20401 cattccattt gttgcaccca tgaaagcagc cagttcgcca ccggcagaat gaccggatac
    20461 tatgatcctg tttacatcaa tattataagt tgctgcattc tttctcaccc agcggatagc
    20521 agatttaata
//
LOCUS       DAORTI010000055        20523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2212_length_20523_cov_6.146619, whole genome shotgun sequence.
ACCESSION   DAORTI010000055 DAORTI010000000
VERSION     DAORTI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_20523_cov_6.146619"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..896)
                     /locus_tag="PLO70_08725"
     CDS             complement(60..896)
                     /locus_tag="PLO70_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQZ74589.1"
                     /translation="MKIYNTTTGIIIGYENRFYHTADNNWDSFINDDGVMEKCQLLIR
                     SGKLRITEMIEDNIILAPVQNQELWACGVTYLISKEGRQEESKEAGGGDFYAKVYEAE
                     RPEIFFKSAPHRIVGHGGKVRIRKDSGWNVPEPELTLVVTSSGKIIGYTIGNDMSSRS
                     IEGENPLYLPQAKTYDGCAALGPCVYVTKQPLPANTMIHMRITRNNDLIFKGSVAISQ
                     MKRSVQELVSFVYRECTFPNGCLILTGTGIVPGNDFTLYPADAISMTIDGIGTLINTV
                     GS"
     gene            complement(913..2238)
                     /locus_tag="PLO70_08730"
     CDS             complement(913..2238)
                     /locus_tag="PLO70_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQZ74590.1"
                     /translation="MAILTIILSIAAIVLLITKLKLHPFLALLLVSIALGLLFGMKTE
                     LIISSIQDGFGGTLGKIGLVIILGVMIGAFLENSGGALVLANKILSIIGQKRVATAMG
                     IIGYIISIPVFSDSGFVLLSSLNRTLTKKAGLSLAGTAIALGLGLSCTHALVPPTPGP
                     VAAAGILNADLALVMGIGLIAAGLALIAALVFAHKVAARVFIDPGPLKENDHTAKDHQ
                     PGLFLSSLPILIPVILIVLKSVTASSSAEGWYTIKQVLAFLGEPVIALLIGFALCLLL
                     PKKLDKNMLSTEGWVGKALKDSATILLVTGAGGIFGKILQNSDIAGMLGEALTQSSIG
                     IFLPFLLAAALKSAQGSSTVALITTASVIAPLMATLGFTSEMDKAMVVVAIGAGSLVV
                     SHVNDSFFWVVTQMSGMDVKTGYKLHTTGTFIIGTTAMIALYLMFCFFH"
     gene            complement(2249..3250)
                     /locus_tag="PLO70_08735"
     CDS             complement(2249..3250)
                     /locus_tag="PLO70_08735"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQZ74591.1"
                     /translation="MKKILLLLYIGFFAGCSAAQQTKTTGKVERIDPSLDAIIDSNAR
                     PVIIAEGFEWSEGPIWIEKHQMLLFSDVPMNTIYKWTKKSGKEIYLEPSGYTGKEPSK
                     SKEPGSNGLTLDIAGNLVLCQHGNRQMAKMDAPLDKPAPNFITLADQYNDKRFNSPND
                     AVSNSAGELFFTDPPYGLPLQNDKDPSKEQPFNGIYKLKKDGKVILLADSITRPNGIA
                     FFPGEQQMIVANSDPNKPNWYTWDIKGDSLVNGRLFYSTLGYDKSWKGLPDGLKIDSK
                     GNVFATGPGGIWILNSKGKLLGKIKLEEACSNVALSDDEKIIYITNDMYLLQLKMRK"
     gene            complement(3261..5015)
                     /locus_tag="PLO70_08740"
     CDS             complement(3261..5015)
                     /locus_tag="PLO70_08740"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HQZ74592.1"
                     /translation="MKQEKNNTSLSGTGGKLRSQDWFGKKNKDGIIYRSWMKNQGMPT
                     DMFDGRPVIGICNTFSELTPCNAHFRDHAEMVKRGVLEAGGFPLEFPIMSLGETLLKP
                     TAMLFRNLASMDAEESIRGNPIDGVVLLTGCDKTTPSTVMGAASVGLPTIVVPGGPML
                     NGRYKGQTIGSGTHVWKFDEDMKTGKMTQEECEFAESCMSRSIGHCMTMGTASTMACM
                     VESLGLTLSGASAIPAADSRKKQMAQLSGRRIVEMVNENLTIDKILIREAFENAIMVN
                     AAVGGSSNFIIHLTAIAGRIGIELTLDDFDSIGSKIPLLLNLMPSGKYLMEDFYYAGG
                     LPVILHELKNKLHKNVVTVTSKNHHENINGNTECFNDDVIATYNKPLIPEAGCVVVKG
                     NLALNGAVIKPSAATPALMKHKGRAVVFESIEDYNARIDDPGLDIDQNCVMVLKYVGP
                     VGYPGMPEVGNMALPKKILEKGIKDMVRISDGRMSGTAYGTAVLHVSPESAIGGNLAL
                     VQNGDMIELDVTQRKLHLHVSDEELEKRRATWTQPKPAAERGYVSMYINHVMGADKGA
                     DLDFLQGSSGSVVTRDSH"
     gene            complement(5030..6157)
                     /locus_tag="PLO70_08745"
     CDS             complement(5030..6157)
                     /locus_tag="PLO70_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ74593.1"
                     /translation="MDYMQKRSRRKFIYNLGMAGLGIPFLSAAANCDNESSKQQTMQS
                     KKAKLGIALVGLGGYAGGQLAPALQQTEHCYLAGIVTGTPSKIPVWKEKYNIPDKNIY
                     NYDNYDSIKDNPDIDIIYVVLPNNMHAEYTIRAAGAGKHVICEKPMAITVEDCDRMIA
                     ACNKANVHLSIGYRLHFEPYNLEMVRCGTKKIYGEIKKLGGGFGFVARPSQWRLTQKY
                     AGGGPLMDLGIYVIQGMCYTTGMLPIAVTAQEGTKTDFERFKEVEQSLTWQFEFPNGL
                     IAEGKTSYNDSHNFLHAEAEQGVFELTSAYNYTGQRGSTPAGVMNFPHINQQAKQMDG
                     IAMAIRNNKKSIVPGEMGKRDVNYLQAVYEAMRTGKKIEIK"
     gene            complement(6218..6475)
                     /locus_tag="PLO70_08750"
     CDS             complement(6218..6475)
                     /locus_tag="PLO70_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74594.1"
                     /translation="MKKIIFFLSPLLLIICAIAQSEQPYYHSFEEMKAWVEKVIQIDS
                     LLVLLFHGVGGGNSLDVSVSVAVFRQRLLYINQKTGPRQPR"
     gene            complement(6479..8398)
                     /locus_tag="PLO70_08755"
     CDS             complement(6479..8398)
                     /locus_tag="PLO70_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQZ74595.1"
                     /translation="MKIFPTIMLLWLTAFSTAAQVKLPKLISDSMVLQRDSKVNVWGW
                     ATANEKVIVKFNNKSYKTKADDSGKWRVVLPATNAGGPHTIIITGKNKITLKEILFGD
                     VWFCSGQSNMVHQMNIHDVTYAKEIAEANYPQIRQFLIPTLSNLQGPQNDLPAGSWKP
                     AVGEDVRPFSAVAYFFAKKLFERYKVPIGIINASVGGTPIESWTSEDGLKDFSTLSAT
                     IQKNKDTAYISAQKNRPQVNTSRQQSDMGLSGKTKWYELSYIPKGWRNIAIPGYWEDQ
                     GIKDLNGVVWYRKEIDVPESMTGKPAKVFLGRIVDADILFINGKQVGATTYQYPQRRY
                     TVPDDLLKTGKNLVVVKVINTFGKGGFVPDKPYYLFAGNDTIDLKGYWQYKVGEVFTP
                     RSNAGGGGIAAQNQPAALYNAMISPVTNFTIKGFLWYQGESNAGRPEEYAKLQPAQII
                     DWRNKWKQGELPFLYAQLPGFMDYNYLPAESQWATLRESQLKSLSVPNTGMAVTIDLG
                     EWNDIHPDNKKDVGERLALLAEKIAYGENIVYSGPLFESSKIESNKIIITFKNTGSGL
                     TSNDGEELSEFAIAGADKKFFWAKAKIEGDKIIVWNEEIKDPVYVRYAWADNPVNPNL
                     YNKEGLPASPFRTDQ"
     gene            complement(8452..9951)
                     /gene="araA"
                     /locus_tag="PLO70_08760"
     CDS             complement(8452..9951)
                     /gene="araA"
                     /locus_tag="PLO70_08760"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HQZ74596.1"
                     /translation="MNKSIHIKMLEVWFATGSQHLYGPETLKQVAQHSAIVSKHLNNE
                     EEIPVKVVFKDVLKSTEEIYNLCLDANHNKNCIGIITWMHTFSPAKMWINGLKILQKP
                     LLHLHTQFNRDIPWGEIDMDFMNLNQSAHGDREFGFMMTRMRMTRKVITGHWQNKEVV
                     HQIAGWTRAALGWNDWQGAKFVRFGDNMRYVAVTDGDKVEAELQFGYSVNTHGIGDLA
                     GFIQSVRETSVTKLCEEYTALYKMIPALKKGNPKHGALRDAAKIELGIRKFLETGNYK
                     GFTDTFEDLHGLVQLPGIAAQRLMQDGYGFAGEGDWKTVALVRAMKVMGTGLPGGNSF
                     MEDYTYHFEPGNEMVLGSHMLEICPSIAVSKPSCEIHPLGIGGKADPVRLVFNVGAGP
                     ALNASLIDMGNRFRLVVNTVEAVKPKYELPELPVARVLWKPHPDMQTGCTAWILAGGA
                     HHTCYSQNLSAEIIEDFAAMAGIEYVKIDQQTNLYQFKNELRWNEAVYK"
     gene            complement(9959..11974)
                     /locus_tag="PLO70_08765"
     CDS             complement(9959..11974)
                     /locus_tag="PLO70_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQZ74597.1"
                     /translation="MKRSLLAILSIISVHCIAQQKDYAIKEINFTRVKLTDKFWLPRI
                     ETNRTITIPASFQRCENTGRVRNFLMAAEKKGKFCTRYPFDDTDIYKTIEGASFSLAT
                     YPDKKMDAYIDSLIILIKNAQEPDGYLYTARTIDPLNVGSWVGKDRWEKERELSHELY
                     NAGHLYEAAAAHYQATGKRSLLDIALRNADLVCSVFGPGKRIVAPGHEVIEMGLVKLF
                     RITGKQEYLNTARFFIYARGLYKGYDAKSKDVWKNGAYWQDDKRVVEQDEAVGHAVRA
                     GYLYAAVADIAALTDDDSLRKAIDKIWQNVVTKKIYVQGGIGAIGDGERFGANYELPN
                     STAYNETCAAIANVYWNYRMFLLHGDSKYIDVLERSLYNGLLSGVGLDGKSFFYTNAM
                     EVKDHFSHKDLERERAGWFECSCCPTNICRFIPAVPGYAYAQKENELFVNLFISSTAS
                     LLMHQQPIEIIQENNYPWDGALSLSIKTKKPNTFNLCIRIPGWAGNTVVPSDLYRFEN
                     NAVSNPGIKINSIPVAYEIKNGYAVISHTWKNNDKVTVELPMEVKRVVCIDSVKDNQG
                     KVALQRGPIIYCAEWADNKGKAGNILLPRDSKFITEYRPDLLNGIMTIKTEVPVVTIS
                     ENENISTSKRTMTAIPYYSWANRGVGEMTIWFPYRVQDIEIFSGKIK"
     gene            complement(11989..13128)
                     /locus_tag="PLO70_08770"
     CDS             complement(11989..13128)
                     /locus_tag="PLO70_08770"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQZ74598.1"
                     /translation="MRSFFIIFVSVLLFTVSSCKSKKSDNPVAPASAEKRLSEKFFGT
                     MGTDTIIEYTLTNTGRMQVSIINYGGTVTSIKTKDKEGKTGDVILGYKSLDGYLQKEN
                     PYFGCLVGRFGNRIANAKFNLEGKTYTLAVNDGSNSLHGGNKGFDKVYWKADKQPGDS
                     SIKLTYISKDNEEGYPGTLTTEVIYTLTSANELKIDYSAITDKPTPVNLTNHCYFNLS
                     AGTDSTILNHLLVLNAAKFTEVNDALIPTGILPDVKGTPMDFTSAKQIGKEIAAVKGG
                     YDHNWVLNKKSNALEFAGSLYHPGSGRLMEVFTTEPGIQFYSGNFLNGTLQYTRHGQK
                     YMKHAGLCLETQHFPDSPNQPSFPNTILKPGEKYQQTTVYKFSLK"
     gene            complement(13154..13852)
                     /gene="araD"
                     /locus_tag="PLO70_08775"
     CDS             complement(13154..13852)
                     /gene="araD"
                     /locus_tag="PLO70_08775"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HQZ74599.1"
                     /translation="MNKYEHIKQEAFEANCQLPGLGLVLFTFGNVSAADKSLGVFAIK
                     PSGMLYEKLSPGNMVIVDFEGKKVEGALRPSSDTLTHAVLYKQWENIGGIAHTHSIYA
                     SAWAQALRSIPLYGTTHADYTTGNIPCTTPMSDEAIKDNYEYATGMQIIHCFKENGYN
                     YEETEMILVGSHAPFTWGKNAAKAVHNSAVLEYIAKMATLTEQVNPQANRLKEALIKK
                     HFERKHGPDSYYGQ"
     gene            complement(13881..15524)
                     /locus_tag="PLO70_08780"
     CDS             complement(13881..15524)
                     /locus_tag="PLO70_08780"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HQZ74600.1"
                     /translation="MLQKKYVIGIDYGTDSVRATLLDAGNGQEIATSVFYYPRWKEGK
                     YCNASASQFRQHPLDYLEGLEATIKHCLKEAGPSISLLVKGISVDTTGSTPVAVNKQG
                     VPLAMLSGLEENPNAMFVLWKDHTANKEADELNRHAEKFPVNYLKYVGGIYSSEWYWA
                     KLLHLLREDESVRKNTYSFVEHCDWIPFLLTGGNDATKIKRGICSAGHKALWAEEFGG
                     LPPEEFFISLDPLLKGFTKSSFLHTYTADQPAGSISKEWSERLGLPEDVVIGIGAFDC
                     HMGAVGGQIEPYYLSKVMGTSTCDMLVAPVHEVKDTLVRGICGQVNGSIIPGMVGIEA
                     GQSAFGDVYAWFKELIAWPVNIFINDEKQQQEITDSIIAKLADAAAVLPLNEADELAL
                     DWMNGRRTPDANQLLKGALTNINLGSDAPRIFKALVEATCFGSKAIVDRFTGEGIPVK
                     GIIALGGVAKKSNYVMQVMTNVLNMPIRIHRSEQTCAGGAAMFAATAAGIYKKVEFAM
                     EAMGHGFEKTYYPEKEKTGYYETRYKMYRDLGNKIEVRQ"
     gene            complement(15524..16180)
                     /locus_tag="PLO70_08785"
     CDS             complement(15524..16180)
                     /locus_tag="PLO70_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152269862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQZ74601.1"
                     /translation="MKNTRAFLFDLNGTMIDDMNYHIRAWHDIFNRLGVTISMHETKM
                     QCYGKNAEVIERVIPGRFSDDEKNTMSLNKEKKYQEAFRPFLKLINGLDDFLEKAKQQ
                     DIQMAIGTAAIRFNVDFVLDGLHLHDYFNVIISADDVEESKPNPETFLKCAELLGVRP
                     DECIVFEDAPKGAEAAERAGMRSVVITTMHKEEEFTGNNIICFAQDYTNGLFEKIMRV
                     "
     gene            complement(16334..17872)
                     /locus_tag="PLO70_08790"
     CDS             complement(16334..17872)
                     /locus_tag="PLO70_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74602.1"
                     /translation="MRILFLLTGVFSTQLLMAQNEIVLNADKGKDTINKNIYGHFAEH
                     LGSCVYGGLYVGEDNKKIVHQNGIRLDVVNALKKLKATVLRWPGGCFADNYHWKEGIG
                     PKEKRRHIENVAWGNVREDNSFGTHEFLQLCEMIGAEPYLAVNMGSGTVQEAAEWVQY
                     VNHANGASDLTDWRQQNGRTNPWGVKYWGVGNESWDCGGHMTVDYYINQYKKYATFMT
                     SYGNSEGLFRIAVGPGWEDFKWTEALMRDIPLKMLDGVSIHHYSVINWDKKGSATGYT
                     ENEYFSTMKQAWRMEKMVAGNSEVMDKYDPEKKIALIVDEWGGWYDVEPGTNKAFLHQ
                     QNTMRDAMIAGISLNIFNNHCDRVKMANLAQIVNVLQAVILTKEEKLILTPTYHVMEM
                     YNVHQDGLHLPVEVNSNKYILGKDTLNAISASASKDKNGVTHISLVNIDAAKTQTLSI
                     TVNGANYKTVSGRILTSDKLQNYNSFDNPNKIVPGVFNGAQLKGSKLSLNIPPFSVVV
                     LDLK"
     gene            complement(17869..20484)
                     /locus_tag="PLO70_08795"
     CDS             complement(17869..20484)
                     /locus_tag="PLO70_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74603.1"
                     /translation="MKIIRSIVIVFLTSNLSAQDYTSYPMWNPSLPFEQRVNDVVNRL
                     TLDEKVAQMLNATPAIPRLGIPAYDWWNEVLHGVARTPFHTTVFPQAIGMAATWDTNS
                     LYQMADYSALEGRAIHNKAIELGRTNERYLGLTYWTPNINIFRDPRWGRGQETYGEDP
                     FLTAMLAKAFVRGLQGEDPKYLKAAACAKHFAVHSGPEPSRHSDNFNPTAYDLWDTYL
                     PAFKELVVDAQVAGVMCAYNAVNTQPCCANDLLMNNILRTQWKFNGYVTSDCWAIDDF
                     FRYHKTHKDATVAAVDAVVHGTDVECGQTVYKTLVEAVKNGLIKEQQLNISLNRLFMI
                     RYRLGMFDPVSMVKYAQTASSVLESATHKAHALKMARQSIVLLRNQHNTLPLKKTIKK
                     IAILGPNADNRIAVLGNYNGIPSKVTTVLEGIREKLGKEVEIIYQKAINLTNDTLLVY
                     TDLSNQYTWEGKQGFKAEYFDNREIKGAPIAVAHEAAVDHLWQRGDIIAGSLKATNFS
                     ARYTTNFKAIADGNVTFEVDADDGYRLIINGIEVLNAWLRNRWGAKTFRLDVKKDSVY
                     NLVLEYWQGDDNASVSLKTGNFVRTDFTAVAGQVKDADAIIFVGGISPQLEGEEMPVN
                     APGFNGGDRSSILLPAVQTELMKALKATGKPIVFVMMTGSAIATPWESENIPAIINAW
                     YGGQSAGTAIADVLFGDYNPAGRLPVTFYKSDSDLPSFMSYDMSGRTYRYFKGEALYP
                     FGYGLSYSSFKYDRLNVPATFKTGKNITVSVRVTNSGKKDGEEVVQLYLAHQNKTNLA
                     PLKALKGFQRIFLKSGESKTIRLELTPEQLSLVNSNGKMYQPKGNIQVSVGGGQPGVK
                     NKTTSNVITANISIL"
BASE COUNT         5334 a         4694 c         3839 g         6656 t
ORIGIN      
        1 ttattgcaag ggtaaacagg atactgataa ttcttttcat tgtttatgca tttatatggt
       61 tacgatccta ctgtatttat taatgttccg atcccgtcaa ttgtcataga tatagcatca
      121 gccggatata aagtgaaatc attacccggt acaattcctg ttccggtcaa gataaggcag
      181 ccattgggaa aagtacattc acggtacaca aaggaaacca gttcctgtac cgatcttttc
      241 atctggctga tggcaacaga tcctttaaaa atgagatcat tgtttcttgt gattctcata
      301 tgtatcatcg tgttagcagg taatggctgt ttagtaacat atacgcaggg gcctaatgct
      361 gcacagccat cataggtttt tgcctgtggc aggtataaag gattttctcc ttctatactg
      421 cggctgctca tgtcgttgcc aatagtataa ccaattattt ttcccgaaga agtaacgaca
      481 agtgtcagtt ccggttctgg cacattccag ccggagtctt ttcgtatcct gacttttccg
      541 ccatgcccca cgatacgatg aggagcagat ttaaagaata tttccggtct ttcagcctcg
      601 tacactttcg cataaaagtc accaccaccg gcttctttgg attcttcctg ccgtccttct
      661 ttactgatca aataagtgac gccgcaggcc cataattcct ggttttgtac tggagcaaga
      721 atgatattat cttctatcat ctctgttatc cggagtttac ctgagcggat gagtaactgg
      781 catttttcca ttactccatc atcattgata aagctgtccc agttattatc ggctgtatga
      841 tagaaccggt tttcataacc gataatgata cctgtagtag tattatatat tttcattttt
      901 ccagtttgat tgttaatgaa agaagcagaa cataaggtac aaggctatca ttgcagttgt
      961 tccgatgata aatgttcctg tggtatgcaa tttatagcct gttttaacat ccatgccgct
     1021 catctgcgtc accacccaga aaaaactgtc gttaacatgt gacaccacta gcgaaccggc
     1081 accgatagca accaccacca tggctttgtc catttcactg gtaaaaccta atgtcgccat
     1141 aagcggtgca atcactgatg ctgttgtaat taatgctacc gttgatgaac cttgtgctga
     1201 ttttagtgcg gccgccagca gaaagggaag aaagatgcca atgcttgatt gggtgagtgc
     1261 ctcaccgagc attccggcta tatcagagtt ctgtaatatt ttaccgaata tgccaccggc
     1321 tccagtcact aacaatatag ttgctgaatc ttttaatgct ttaccaaccc atccttctgt
     1381 tgaaagcatg tttttgtcta gcttttttgg cagcaacaga caaagggcaa aacctatcag
     1441 taaagcgata accggttcac ctaaaaatgc aagtacttgt tttatggtat accatccttc
     1501 tgcagatgat gaagctgtga ccgattttaa aacgatcaat atcaccggta taagaatagg
     1561 caaagatgaa agaaacaaac cgggttggtg atctttcgca gtatgatcat tctctttcaa
     1621 tgggccgggg tcaataaaaa ccctggcggc aactttatga gcaaatacaa gggcggctat
     1681 caatgcaaga ccggctgcta tcaaacctat gcccattacc agggccagat cagcatttaa
     1741 gatacctgct gcggctacgg gaccgggagt agggggtact aatgcatgtg tgcaggacag
     1801 gcctaatcct aatgcaatag cggttccggc taatgataat cctgcttttt tggttaatgt
     1861 tctgtttagc gaagaaagca acacaaaacc actgtcggaa aaaaccggga tagaaattat
     1921 ataaccaata atacccatcg ccgttgcaac tcttttctga ccaatgatag ataatatttt
     1981 gttggccagc accaatgcac ccccgctgtt ttccaaaaat gcaccgatca ttactcccaa
     2041 aataataacc aaccctatct ttccaagtgt accaccaaaa ccatcttgta tagagctgat
     2101 gataagctcc gttttcatcc cgaataataa tccgagagca atactcacca gcagtaatgc
     2161 aaggaaagga tgcagtttca gctttgtgat aagcagcaca atagcagcaa tacttagtat
     2221 gatggtgagg atggccatgt ccctaatatt attttctcat ctttaactgc agcagataca
     2281 tgtcattggt gatataaatt attttttcat catcggataa ggcaacatta gaacaggctt
     2341 cctccagttt tatttttccc agcaattttc ctttactgtt caatatccat ataccgccgg
     2401 gtccggtggc aaaaacattt cctttactat cgatcttcaa gccatcgggc agacccttcc
     2461 atgacttatc ataacctaaa gtgctgtaaa ataatctccc gtttaccaat gaatcacctt
     2521 ttatatccca ggtataccaa ttgggtttat tgggatcact gttggccact atcatttgtt
     2581 gttcacccgg gaagaaagca attccattgg gtctggtgat agaatcggcc aggagaatta
     2641 cctttccgtc tttctttaat ttgtaaatgc cgttaaacgg ttgttctttg gagggatctt
     2701 tgtcgttctg cagaggaagg ccatatggag ggtctgtaaa aaataattca cctgcactgt
     2761 tagacacagc atcattagga ctattgaaac gcttgtcgtt atactgatca gccagggtaa
     2821 tgaaattagg tgctggctta tcaagcggtg catccatttt agccatctgc cggttaccat
     2881 gctgacataa tacgaggttg ccagctatat ccagcgttag gccattactg cccggttctt
     2941 tactttttga gggttcctta ccggtatacc cggaaggctc caggtatatt tcctttccac
     3001 tttttttcgt ccacttataa atggtattca taggtacatc tgagaaaagc agcatttgat
     3061 gcttttctat ccatattggt ccttcactcc attcaaaccc ttctgcaatg atcactggcc
     3121 ttgcattact gtctatgata gcatcgagtg aagggtcaat tcgttctact tttccggttg
     3181 ttttggtttg ttgtgccgcg gaacaaccgg caaagaatcc tatgtataac aacaataata
     3241 tttttttcat gttgatcggt ttagtgtgaa tctctggtaa caactgagcc cgaacttccc
     3301 tgtaaaaaat cgagatcagc ccctttgtca gcacccatta catggttaat atacatactc
     3361 acatagcctc tttcagctgc aggttttggt tgtgtccatg tggcccttct tttttctaat
     3421 tcttcatcac ttacatgcag gtgtagtttt cgctgtgtta cgtccagctc aatcatgtca
     3481 ccattttgca ccaatgctaa attgccgcca atagccgatt ctggcgatac atgcagcact
     3541 gctgtaccat aagcagtacc actcattctt ccatcactga tgcgtaccat atctttaatg
     3601 cccttctcta atattttttt aggtaaagcc atgttgccta cttcaggcat acccgggtaa
     3661 ccgacaggac ccacatattt taataccatt acacagttct ggtcaatatc gagccccggg
     3721 tcatcaatcc ttgcattata gtcttcaatg ctttcaaaca ctactgcacg acctttatgt
     3781 ttcataaggg caggtgtggc tgccgatggt tttatgacag caccattcaa agccaaattt
     3841 ccttttacaa ccacacaacc agcttcaggt attaaaggtt tattgtaagt ggcaatcaca
     3901 tcatcattaa agcattctgt attaccgttg atattttcat ggtgattttt actggtaaca
     3961 gtcaccacat ttttatgcaa tttgttttta agttcatgta atataacagg cagaccacct
     4021 gcatagtaaa aatcttccat caggtatttg cctgaaggca tcaggttcag taaaagaggg
     4081 attttacttc cgatcgaatc aaaatcatcc agtgttaatt caatgccgat cctcccggca
     4141 atagccgtaa gatgaataat aaaattggag gaaccaccaa cagctgcatt gaccatgata
     4201 gcattttcga aagcttccct tatcagtatt ttatcaatgg tcaggttttc atttaccatt
     4261 tccacaattc gtctaccgct taactgggcc atctgttttt ttctgctatc agcagcgggt
     4321 atggctgaag cgccacttaa tgtcaatccc agcgattcta ccatacaagc catcgtggaa
     4381 gctgtaccca tcgtcatgca atgcccgatg cttctactca tacaactttc ggcaaactca
     4441 cactcttcct gtgtcatctt gccggtcttc atatcttcat caaatttcca cacatgtgta
     4501 ccactgccga tcgtctgacc tttgtatcgt ccattcagca ttggtccccc gggtaccaca
     4561 atagtaggca ggccaacact ggcggcaccc atcacagtgg atggagtagt tttatcacaa
     4621 ccagtcaata acaccactcc atcaatcggg tttccacgga tggattcttc cgcatccata
     4681 ctggcaaggt tgcggaataa catggcggtc ggtttcaata aagtttcacc caggctcatg
     4741 attggaaatt ctaacgggaa acctccggct tccaatacgc cacgtttcac catttctgca
     4801 tggtcacgga aatgggcatt gcagggtgtc agttcgctga atgtattgca aataccaatt
     4861 accggtctgc catcaaacat atcggttggc atgccctggt tcttcatcca gctgcggtaa
     4921 atgattccat ctttgttttt tttgccaaac caatcctggc ttcgtagttt acccccggtc
     4981 cctgaaaggg aggtattgtt tttttcttgt ttcatgaaaa ttggaattat tattttattt
     5041 ctattttttt gcctgtccgc attgcctcat aaactgcctg taaatagttt acatcccttt
     5101 tgcccatttc tccgggaaca atgctctttt tgttattcct gatcgccatt gcaattccat
     5161 ccatttgttt tgcctgctgg ttgatgtgcg ggaagttcat tacacctgct ggggtgcttc
     5221 cccgttgccc tgtataattg tatgcagaag ttaattcaaa cacgccttgt tctgcttctg
     5281 catgtaaaaa attatgtgaa tcgttatagc tcgttttacc ttcggcaatt aatccatttg
     5341 gaaattcaaa ttgccaggta agcgattgtt ccacttcttt aaaccgttca aaatcggttt
     5401 tcgttccttc ctgtgcagta actgcaattg gtaacatacc tgttgtatag cacatgccct
     5461 gtattacata aatacccaga tccatcaacg ggccacctcc agcatatttt tgtgtgagtc
     5521 gccattgtga cgggcgggcc acaaaaccaa agccacctcc aagttttttt atttcaccat
     5581 aaatcttttt tgtaccgcaa cgcaccattt ctaaattgta tggctcaaag tgcaggcgat
     5641 agccaatact taaatgcaca tttgctttat tacaggcagc gatcatgcgg tcgcaatctt
     5701 caacagtaat tgccattggt ttttcgcaga tcacatgctt gcctgctcct gcagcacgga
     5761 tggtgtattc tgcatgcatg ttattgggca gcactacata aataatatca atatcgggat
     5821 tgtcttttat ggaatcatag ttatcgtagt tataaatatt tttatccggg atgttgtact
     5881 tttctttcca taccggtatt ttagatgggg tgcctgttac aatacctgcg aggtagcaat
     5941 gctcagtttg ctgcagggct ggtgctaact ggccgccggc gtatccacca agtcctacta
     6001 atgctattcc gagttttgcc tttttacttt gcatggtttg ttgttttgat gattcattgt
     6061 cacaatttgc agcagcagaa aggaaaggaa tacccagccc tgccattcca aggttataaa
     6121 taaattttct ccttgatctt ttttgcatat agtccataac caggatttta atttttaatt
     6181 ccggatagct gttgcctgat atatccggcc acttcgacta tcggggctgt ctaggtcccg
     6241 ttttttggtt gatatacaac aaccgttgcc ggaatactgc tactgatact gatacatcca
     6301 ggctattacc tccacccaca ccatgaaaaa ggagtacaag cagagaatcg atctgtatga
     6361 ctttttcaac ccatgctttc atctcttcaa agctgtggta atagggttgt tcagattgag
     6421 caatggcaca tataataagt aacaagggag acaaaaaaaa tattattttt ttcattgttt
     6481 attgatctgt tctgaaagga gaggcaggta agccttcttt gttatacaaa tttggattta
     6541 ccgggttgtc ggcccatgca tatcttacat atacagggtc ttttatctcc tcattccaca
     6601 caataatttt atcgccttca atttttgctt ttgcccaaaa aaatttttta tctgcgccgg
     6661 cgatcgcaaa ttcacttaat tcttcaccgt cattggaagt cagtccactg ccggtatttt
     6721 taaatgtgat gatgatttta ttactttcaa tttttgatga ttcgaataga gggcctgaat
     6781 aaactatatt ttccccgtat gcaatttttt ccgccaacag ggctaatctt tccccaacat
     6841 cttttttatt atccgggtgg atgtcgttcc attcacccag gtcaatagtg actgccatgc
     6901 ctgtattggg taccgataaa gattttaatt gtgattcccg cagcgttgcc cactgacttt
     6961 cagcaggcag ataattataa tccataaagc cgggcagttg tgcatataaa aaagggagct
     7021 ctccctgttt ccatttattc cgccaatcaa taatttgtgc cggctggagt ttagcatact
     7081 cttctggcct tcctgcgtta ctttcaccct ggtaccaaag aaaacctttg attgtgaaat
     7141 tggttaccgg agatatcatg gcattgtata atgctgctgg ctgattttgt gcagcaatgc
     7201 cgccgccacc tgcattgctg cggggagtaa atacttcacc cactttatat tgccagtaac
     7261 ctttcagatc tatagtatca ttacccgcaa acaaatagta aggtttatcc ggaacaaatc
     7321 ctcctttacc gaatgtattg atcactttta caaccaccag gtttttacca gttttcagca
     7381 gatcatcagg aacagtatat cttctctggg ggtattgata agtagtagca ccgacttgtt
     7441 tcccattgat aaataagata tcagcatcga caattcttcc caggaaaact tttgccggtt
     7501 taccggtcat tgattcgggc acatcaattt cttttctgta ccaaacaacg ccattgagat
     7561 ctttaatacc ctgatcttcc caataaccgg gtattgcaat attgcgccaa cctttgggta
     7621 tataagaaag ctcataccat tttgtttttc ccgataatcc catgtctgat tgctgcctgg
     7681 atgtgttgac ctgggggcga ttcttttgtg cactgatata ggctgtgtcc ttatttttct
     7741 gaatggtggc ggataatgtc gaaaagtcct ttaatccatc ttcactggtc catgattcaa
     7801 tgggtgttcc acccacactg gcattgatga taccaatggg tactttatat ctttcaaaaa
     7861 gttttttagc aaaaaagtaa gcaacagctg aaaaaggccg tacatcttcc cccacggcgg
     7921 gtttccaact tccggcaggt aaatcatttt gcgggccttg taaattggaa agggtaggga
     7981 tcaaaaattg ccgtatctgc ggataattgg cttctgcaat ttcttttgca taggtaacat
     8041 catgaatatt catctggtgt accatattag actgaccact gcaaaaccaa acatcaccaa
     8101 aaagtatctc tttcaacgta atcttgtttt tgccagtgat tattattgta tgtggcccac
     8161 cggcattggt agccggtaat actacccgcc atttgcccga atcatcagct ttagttttat
     8221 aggatttatt gttgaatttt acaatcactt tttcatttgc agtagcccaa ccccagacat
     8281 ttacttttga gtcccgctgc aataccatac tgtcgctgat aagctttgga agttttacct
     8341 gagccgctgt actaaaagca gtcagccata ataacatgat agttggaaag attttcatac
     8401 ctgtattttt tgaatagccc gatgactaat tcgttctaaa ttcttaaagg atcatttgta
     8461 aaccgcttca ttccatctta actcattctt gaactggtag aggttcgttt gttgatcaat
     8521 ttttacatac tctatacctg ccatcgcagc aaaatcctca atgatttcag cgcttagatt
     8581 ctgactgtaa caagtatggt gtgcaccgcc agctaatatc caggccgtgc agcctgtttg
     8641 catatcggga tgaggtttcc aaagtaccct tgctacaggc aattcaggca gctcatattt
     8701 tggctttact gcctccaccg tattaaccac taaccgaaaa cggtttccca tatctattag
     8761 tgatgcattc agtgctggac cggccccgac attgaatacc aaccgcaccg gatcagcttt
     8821 accaccaata cctaatggat gaatttcgca ggatggtttt gatacagcaa tactgggaca
     8881 tatttccagc atatgactgc ccaacaccat ttcattgccc ggttcaaaat gataagtata
     8941 gtcttccata aatgaattac cacccggcag gccagtaccc attactttca tagcccttac
     9001 caatgctact gttttccagt caccttcacc tgcaaaacca taaccatcct gcatcaggcg
     9061 ctgagcagct ataccaggca gttgtaccag gccgtgcagg tcttcaaacg tatcagtaaa
     9121 acctttataa ttccctgtct ctaaaaattt tcttatcccc agttcaatct ttgctgcatc
     9181 ccgtagcgca ccatgttttg gatttccttt ttttaatgcc ggtatcattt tatagagtgc
     9241 agtatattcc tcacacagct ttgtaacgga tgtttccctt actgattgga taaatcctgc
     9301 caggtcaccg atgccatgag tgttcacact ataaccgaat tgtaattccg cttccacttt
     9361 atcgccatca gtaacagcta cataacgcat gttatcgcca aagcgaacaa actttgcacc
     9421 ctgccagtca ttccatccca atgcagccct tgtccatcct gcaatttggt gtaccacttc
     9481 tttattttgc caatgacctg ttatcacttt acgggtcatc cgcatacggg tcatcataaa
     9541 accaaattcc ctgtcaccat gagcactttg gttcaggttc ataaaatcca tatctatttc
     9601 tccccaggga atatccctgt tgaattgtgt gtgcaggtgc aataaaggtt tttgcaggat
     9661 cttcagacca ttgatccaca tttttgcagg cgaaaatgtg tgcatccagg taatgatacc
     9721 aatacaattt ttattgtgat tggcatctaa gcatagattg taaatctctt ctgttgattt
     9781 cagcacatct ttgaagacaa cttttacagg aatttcttcc tcattgttta aatgttttga
     9841 aacaatagca gaatgttgtg ccacctgttt caatgtctcg ggaccgtata aatgctggct
     9901 gcctgtggca aaccatactt ccagcatttt tatgtggatg ctcttattca taatatgctt
     9961 atttaatttt tccgctaaat atctcaatat cctgtacccg gtaagggaac caaatggtca
    10021 tttcaccaac gcccctgttt gcccagctgt aatagggaat ggctgtcatt gttcgtttac
    10081 ttgtactgat attttcgttt tcactgattg taaccaccgg gacttctgtt ttgatggtca
    10141 ttataccatt cagcaggtca ggcctgtatt cagtaataaa ttttgaatcc cggggaagta
    10201 agatatttcc ggctttacct ttattatctg cccattctgc acagtatatt attggtcccc
    10261 gctgcagggc gacttttccc tggttgtctt tcacactgtc aatacaaacg acccttttta
    10321 cttccatagg cagttccaca gttaccttgt cattattttt ccaggtgtga cttatcaccg
    10381 catagccatt ttttatttca tacgctacag gtatactatt tatttttata ccagggtttg
    10441 atacagcatt attttcaaac cggtacagat cagaaggtac aacagtgttt cctgcccaac
    10501 caggaattcg gatgcacagg ttgaatgtat ttggtttttt ggttttgata cttaatgaaa
    10561 gagctccatc ccaggggtaa ttattttcct ggatgatttc aatgggctgt tgatgcatta
    10621 aaaggcttgc tgtactgctg atgaagaggt ttacaaacag ctcattttct ttttgggcat
    10681 aggcatatcc cggtaccgca ggaataaacc ggcagatatt ggtaggacag cagctgcatt
    10741 caaaccatcc tgctctttcc cgttcaaggt ctttatgaga gaaatggtct ttcacctcca
    10801 tggcattggt gtagaaaaag gatttcccat ctagtcctac accagatagt aaaccattgt
    10861 acaggctcct ttccagcaca tcaatgtatt ttgaatcacc atgcaaaaga aacatcctgt
    10921 aattccagta tacattagct atcgcggcgc aagtttcatt gtaagctgta ctgtttggca
    10981 attcataatt ggcgccaaaa cgttctccat caccaatggc gccgatgcca ccctgtacat
    11041 aaattttttt cgttacaaca ttctgccata ttttatctat agcttttctc aggctgtcat
    11101 catcagtcag tgcagcaata tcagccaccg ctgcatataa atacccggct ctaacagcat
    11161 gaccaacggc ttcatcctgt tctaccaccc gtttgtcatc ctgccagtaa gcgccatttt
    11221 tccagacatc tttacttttt gcatcatatc ctttgtaaag acctctcgca taaataaaga
    11281 atcgggcggt gttcaggtac tcctgctttc cggttatcct gaataatttt acaaggccca
    11341 tttctatcac ttcatgtccc ggcgccacta ttcttttgcc ggggccaaaa acagagcata
    11401 ccaggtcagc gttgcgcaac gcaatatcta atagcgatct tttcccggtt gcctggtaat
    11461 gtgcagccgc cgcctcgtat aaatgcccgg cgttgtataa ttcatggctc agttctcttt
    11521 ctttttccca acgatctttt cccacccagc tacctacatt aagcgggtcg atggtccttg
    11581 ctgtatataa atagccatcc ggctcctgtg catttttaat caggatgatc agcgagtcta
    11641 tatatgcatc catcttttta tcgggataag ttgccagtga aaaagaggct ccttcgatcg
    11701 ttttatatat atccgtatca tcgaaagggt accttgtgca aaattttcct tttttttcag
    11761 cagccataag aaagttcctg acacggccag tattttcaca gcgctgaaaa gaagcaggaa
    11821 tcgttattgt acggttcgtt tcaatacgtg gaagccagaa cttgtcggta agtttaaccc
    11881 tggtaaaatt gatttctttt attgcataat ctttttgctg ggctatgcaa tgaacggaga
    11941 taattgatag tatggcaagt aaggatcttt tcatttctta ttcatacatt attttaggga
    12001 aaatttatag actgttgttt gctgatattt ttcacccggc tttaaaatcg tatttgggaa
    12061 tgaaggttgg ttagggctgt ccgggaaatg ttgtgtttcc aggcaaagcc ccgcatgttt
    12121 catatacttt tgtccgtgtc gggtgtattg caatgtgcca ttcaaaaaat tcccgctgta
    12181 aaactgaata ccgggctcag tggtgaatac ctccatcaat cttccgctgc cgggatgata
    12241 caatgaaccg gcaaattcca acgcattact ttttttattg agtacccagt tatggtcgta
    12301 accacctttt acagctgcaa tttcttttcc gatctgtttt gctgaagtaa agtccattgg
    12361 cgtacctttt acatcaggca atattccggt tggtatcagt gcatcattta cttctgtgaa
    12421 tttagctgca ttcagcacta ataaatgatt gagtattgtg gagtcagtcc ctgcagataa
    12481 attgaaataa caatgattgg tcaggtttac aggagttggt ttatcagtaa tggctgaata
    12541 gtctattttc agttcattgg cggaagtcag tgtataaatc acttccgtgg tcaatgtacc
    12601 cggataccct tcttcgttat ctttacttat ataagttaat ttaatactgc tgtcaccggg
    12661 ttgtttatct gctttccagt atactttatc aaatccctta tttccgccat gcaatgaatt
    12721 ggaaccatcg ttaacggcca gtgtatatgt tttcccttcc aggttaaatt tggcatttgc
    12781 aatacggtta ccaaatcttc ccaccaggca accgaaataa ggattttctt tttgcagata
    12841 accgtccagc gacttatagc caagtattac atcaccggtt tttccctcct tatcctttgt
    12901 tttgatactg gtgactgtac ctccataatt tattatgctt acctgcattc ttccggtatt
    12961 ggttaatgta tattctatga tcgtatcagt acccattgtg ccgaaaaact tttctgataa
    13021 tcttttttca gccgaagcag gagcaacagg gttatcgctt tttttgctct tacaggaaga
    13081 cactgtaaat aaaagcaccg aaacaaaaat gatgaaaaat gaacgcattt taaatcttta
    13141 tttagaagtt attttactgt ccgtaataag aatccgggcc gtgttttctt tcaaaatgtt
    13201 tttttatcaa tgcctccttt aaacgattgg cttgaggatt cacctgctct gttaaggttg
    13261 ccatttttgc aatgtattcc agtacggcac tattgtgcac agcctttgct gcattttttc
    13321 cccacgtaaa tggggcgtgg ctacctacta gtatcatttc agtttcttca tagttgtaac
    13381 cattttcttt aaaacagtga atgatctgca tacctgttgc atattcataa ttatctttta
    13441 ttgcttcatc actcattggt gtcgtacagg gaatatttcc ggtagtgtaa tcagcatgtg
    13501 tggttccgta tagcggaatg ctgcgaagtg cctgtgccca tgcagaagca taaatggaat
    13561 gtgtgtgtgc tataccgcca atattctccc attgtttata cagtaccgca tgggtcagcg
    13621 tatcggaaga agggcgaaga gccccttcca ctttcttacc ttcaaaatct acaattacca
    13681 tattacctgg tgacagtttt tcataaagca tgccgcttgg tttgatggca aatacaccga
    13741 gtgatttgtc agcagcacta acattaccaa atgtaaataa caccaatcct aatccgggca
    13801 attgacagtt ggcttcaaaa gcctcctgtt tgatatgttc atatttattc ataataaata
    13861 acctcctgct cagttcttat ttattgtctg acttcaattt tattaccaag gtcccggtac
    13921 attttatacc tggtttcata atatccggtt ttttcttttt caggataata ggttttttca
    13981 aagccatgtc ccattgcttc catggcaaat tctacttttt tataaatacc ggctgccgtt
    14041 gctgcaaaca tggctgcacc cccggcacag gtttgttcgc tccgatgtat gcgtatgggc
    14101 atattgagca cattggtcat tacctgcatt acataatttg attttttggc tactcctccc
    14161 aatgcaatga tgccctttac cggaatacct tccccggtaa atcgatctac gattgcttta
    14221 cttccaaaac aggttgcttc taccagtgct ttgaatattc tcggtgcatc gctgcccaga
    14281 ttgatattgg ttaaagcacc tttcaataac tggttggcat caggagtacg acgcccattc
    14341 atccagtcca gtgccagttc atctgcttca ttcaatggca atacagcagc tgcatcggcc
    14401 agctttgcaa taatgctatc agttatttcc tgttgttgtt tttcatcatt gataaaaata
    14461 ttaacaggcc aggcaataag ttctttaaac caagcatata catctccaaa tgcactttga
    14521 ccagcttcaa tacctaccat accggggatg atggagccat tcacttgtcc gcaaataccc
    14581 cttaccagcg tatcttttac ttcatgtaca ggggcaacca gcatatcaca ggttgaggta
    14641 cccattactt tactcaggta ataaggttca atttgtccgc ccacagctcc catatgacaa
    14701 tcaaaagcac caattcctat aactacatct tcaggcaagc ccaacctttc actccattct
    14761 ttactgattg acccagccgg ctggtctgct gtatatgtat gcaggaaaga gctttttgta
    14821 aatcctttca gcaaaggatc aagtgaaata aagaattctt caggtggtaa tccaccaaat
    14881 tcttctgccc acagggcttt atgcccggca ctgcatattc ctctttttat ttttgttgca
    14941 tcattgccac cggtcagcaa aaaaggtatc cagtcgcaat gttccacaaa cgaataggta
    15001 ttttttctca cggattcatc ttcccggagc aagtgaagta atttagccca ataccattct
    15061 gatgaataaa taccgcctac atacttcaag tagttaaccg gaaacttttc agcatgcctg
    15121 ttaagttcgt ccgcttcttt gttcgctgta tgatctttcc ataatacaaa catggcgttg
    15181 gggttttcct ccaggccgct cagcatagca agcggtaccc cttgcttatt tactgccaca
    15241 ggggttgatc cggtagtgtc taccgagatt ccctttacca gcagtgagat ggatggtccc
    15301 gcttctttca aacagtgctt aatagttgct tccaggccct ccaggtaatc caacggatgc
    15361 tgcctgaact ggctggctga agcattacaa tattttcctt ctttccacct gggatagtaa
    15421 aaaacagaag tggctatctc ttgtccgttt cctgcatcga gaagtgttgc ccggacagag
    15481 tcagtaccat aatcaattcc tattacatat tttttttgaa gcattatact ctcattattt
    15541 tttcaaacaa cccatttgta taatcctggg caaaacaaat aatattatta ccggtaaatt
    15601 cttcttcctt gtgcatagtt gttattacga ccgatcgcat gcctgctctt tcggctgctt
    15661 cggccccctt gggagcatct tcaaatacaa tgcattcatc gggtcgtaca cccagtaatt
    15721 cggcgcattt aagaaatgtt tccgggttgg gtttactttc ctctacatca tctgcactga
    15781 taattacatt aaaatagtcg tgtaagtgca ggccatccaa cacaaagtcg acattgaatc
    15841 ttatagctgc tgtcccgatc gccatctgta tatcttgttg tttggctttt tcgagaaaat
    15901 catcaagccc gttaataagt tttaaaaaag gcctgaaagc ttcctggtat tttttttctt
    15961 tattcaggct catcgtattt ttttcatcat cagaaaacct gcccggtatt accctttcaa
    16021 ttacttctgc atttttccca taacactgca ttttggtttc atgcatagaa atagttactc
    16081 cgagccggtt aaaaatatca tgccaggccc tgatatgata gttcatatca tcgatcatag
    16141 tgccgtttaa atcaaataag aatgccctgg tatttttcaa gtgtctggtt atttatcggg
    16201 tttttaattt ttttatacat ttttattgaa tattacggta ccagttttgt ctctgcggta
    16261 aaaccaagtt cctgattttc tttctgtttt tcaggaagta taagctatgc ctggtaatgt
    16321 attatttcct ttttcatttt aagtcaagca ccactacact gaacggcggt atatttaagc
    16381 tcagttttga accttttaat tgtgccccat tgaatacacc cggaacaatt ttattagggt
    16441 tatcaaagct gttataattc tgcagtttgt ccgatgtcag aattcttccg ctgactgttt
    16501 tataatttgc tccattgaca gtaatactta gtgtttgtgt tttggccgca tcaatattta
    16561 ccagtgaaat atgcgttaca ccatttttat cttttgaagc tgatgcagaa atagcgttta
    16621 aagtatcttt tccaagaata tatttattgc tattgacttc aaccggcagg tggagtccat
    16681 cctggtgtac attgtacatc tccatcacat gataagttgg tgtaagtatt agtttttctt
    16741 ctttggtcaa tattacagct tgcaatacgt taacgatttg tgccaggttc gccatcttta
    16801 ccctgtcgca gtggttattg aaaatattta aactgattcc ggcgatcatt gcatcccgca
    16861 tggtattttg ctggtggaga aatgctttat tggttccggg ttccacatca taccagcctc
    16921 cccattcgtc cacaatcagt gcaatctttt tttccggatc gtacttgtcc atcacttcgc
    16981 tgttaccggc gaccattttt tccatacgcc aggcttgttt catcgttgag aaatattcat
    17041 tttctgtata cccggtcgct gatccttttt tatcccagtt gataacagag taatggtgaa
    17101 tagaaacccc atcaagcatt ttaagcggga tatccctcat taatgcttca gtccatttaa
    17161 aatcttccca tcccgggcca actgctatcc ggaacaatcc ttccgaattt ccgtagctgg
    17221 tcatgaatgt cgcatatttt ttatactggt taatgtaata atcaacagtc atatgtccgc
    17281 cacagtccca tgattcattg cctactcccc agtattttac cccccaggga tttgtcctgc
    17341 cgttttgctg acgccaatcc gtaagatcgc tggcaccatt ggcatgattt acatattgca
    17401 cccattcagc tgcttcctga acggtaccac ttcccatatt gacagcaagg taaggttcag
    17461 caccgatcat ttcacaaagt tgtaagaact catgggtgcc aaaactgttg tcttctctta
    17521 cgttgcccca tgctacattt tcaatatgcc ttcgtttttc ttttggtcct ataccttctt
    17581 tccagtgata attatcggca aaacagccac ccggccagcg cagtacggtt gcttttagtt
    17641 tttttaatgc attcacaaca tcaagccgga taccattttg atggacaatt tttttattgt
    17701 cttcacctac atacagtcct ccatatacac aactgcctag gtgctcagcg aaatggccgt
    17761 agatattttt attgatagtg tctttccctt tatctgcatt caatacaatt tcattttgcg
    17821 ccattaacag ctgtgtactg aatacgccgg ttagtagaaa taagattctc ataaaattga
    17881 aatatttgca gttattacat tactggtagt tttattcttt acaccgggtt gccccccgcc
    17941 aacactcacc tgaatgttgc ctttaggctg gtacatcttt ccattactat tcaccaatga
    18001 tagctgttct ggagtcagct ccaatcttat cgttttgctc tccccgcttt ttaaaaatat
    18061 tcgttgaaaa ccttttaatg ctttcagcgg tgcaagattt gttttattct gatgtgccaa
    18121 atacaactgc actacttctt cgccatcctt ttttccgcta ttggtaaccc gtactgaaac
    18181 cgttatattt ttcccggttt taaaagtagc cggaacgttc agcctgtcat atttaaaact
    18241 actataactt agtccatacc caaaaggata taacgcttca cctttgaaat aacgataggt
    18301 tctgccggac atatcgtaac tcatgaaaga agggaggtca ctgtcactct tataaaaagt
    18361 aaccggtaaa cggccagcgg ggttatagtc tccaaataat acatcagcga tagctgtacc
    18421 ggcactttgt ccaccgtacc aggcattgat aatggcagga atattttcgc tttcccatgg
    18481 agttgcgata gcactgcctg tcatcatcac aaatactatt ggcttaccgg tagctttcag
    18541 tgctttcatc agttcagttt gtacagcagg aagtaaaata ctgcttctgt cgccaccatt
    18601 aaaaccaggt gcattgacag gcatttcttc accttccagt tgcggagaga tacctccaac
    18661 aaatatgatg gcatctgcat cttttacctg gccggctacg gcagtaaaat ctgtacggac
    18721 gaaattgcct gtctttaatg atacgctggc attatcatcg ccctgccagt attccaatac
    18781 aaggttgtat acagaatctt tttttacatc aagcctgaat gtttttgccc cccatcggtt
    18841 acgcaaccag gcattgagta cttctatgcc atttatgatt aatctatatc catcatctgc
    18901 gtccacttca aatgtaacat tcccatcagc gatcgccttg aagttggtcg tataccttgc
    18961 tgaaaaatta gtagctttta aacttcctgc tatgatatct ccccgctgcc ataaatgatc
    19021 aacagcagct tcatgggcta ccgctatggg agcacctttt atttcacggt tatcaaagta
    19081 ttctgctttg aatccctgtt tgccctccca ggtatattga ttgcttaggt ccgtatatac
    19141 taataaggtg tcattggtca ggttaatagc tttctgataa atgatttcta cctctttgcc
    19201 caacttttcc cggatgccct caagcaccgt tgtaactttt gagggaatac cattgtaatt
    19261 tcccagtact gcaatccggt tgtcggcatt cgggcccagt atagcaattt tctttatagt
    19321 cttttttaat ggtagtgtat tatgctgatt ccgtagtaaa acgatagatt gccgggccat
    19381 ctttagagca tgtgctttat gggttgcact ctccaaaacg gaagaagctg tttgtgcata
    19441 tttcaccatt gataccgggt cgaacatgcc caggcgatag cggatcataa ataatcggtt
    19501 cagtgaaata ttgagttgtt gttctttgat caaaccattt ttcacagctt ccacgagtgt
    19561 tttataaact gtctggccac attccacatc tgtgccatga accactgcgt ctacggctgc
    19621 taccgtggcg tctttatgtg ttttatgata acgaaagaaa tcatctatgg cccagcagtc
    19681 ggaagtgaca tacccgttaa atttccattg tgtgcggaga atattattca tcaacaggtc
    19741 gtttgcacaa cagggttggg tattcactgc attataagca cacatcacgc cggctacctg
    19801 agcatcaacg accagttctt tgaatgcagg taagtaagtg tcccataaat catacgccgt
    19861 tggattgaag ttgtcggagt gtcttgacgg ttcaggaccg ctatgcactg cgaaatgttt
    19921 ggcacaggct gcagctttta ggtattttgg atcttcaccc tgcaatcctc ttacaaaagc
    19981 ttttgccagc attgccgtca gaaaagggtc ttcaccataa gtttcctggc cgcggcccca
    20041 gcggggatca cggaaaatat tgatattggg tgtccagtaa gtaagtccca gatatctctc
    20101 atttgttctg ccgagttcaa ttgctttatt gtgaatggct cttccctcca acgcagaata
    20161 atcagccatt tggtataatg aattggtatc ccatgttgct gccatcccaa tggcctgcgg
    20221 gaatacagta gtgtgaaatg gagttcttgc tacgccatgc aatacttcat tccaccagtc
    20281 ataagcgggg ataccaagtc gtggaatagc tggtgtggca ttcaacattt gggcaacttt
    20341 ttcatcgagt gttaaacgat tgaccacatc atttacccgt tgttcaaaag gtaatgatgg
    20401 attccacata ggataagaag tatagtcctg tgcggaaaga ttacttgtca ggaaaacgat
    20461 tacgattgaa cgaattattt tcatacgctt tagttaataa ataaaaactt tttacatcac
    20521 tcc
//
LOCUS       DAORTI010000056        20429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2229_length_20429_cov_9.854962, whole genome shotgun sequence.
ACCESSION   DAORTI010000056 DAORTI010000000
VERSION     DAORTI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20429
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_20429_cov_9.854962"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLO70_08800"
     CDS             complement(<1..706)
                     /locus_tag="PLO70_08800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQZ74604.1"
                     /translation="MNPKKSIAFALCFLVLQSCSDKFYAGRKKTVVVWQPSHQTDTGK
                     DFSEAAVCNAIAEAAMKTNPRLKEYKVWSLGKTEYHHADSGSNTKILHTSAVIDGKIS
                     GYAFELQASNKLKPSIFISVHNNGGTKRNAVWGYIHYGDKYEKDNRELAALLIKAISE
                     VSGLENRGVLLDSTTGRNDYRCQATGKLSFYSLDEHINTAPYRVLLEIGDNAVSRELL
                     LNPDSQQKMGAAIKKAL"
     gene            902..1570
                     /locus_tag="PLO70_08805"
     CDS             902..1570
                     /locus_tag="PLO70_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74605.1"
                     /translation="MHPDQSDSTARHKEPNDQIISYYTLRVLIGATGVLLPLLVFFGK
                     WISESSGVLEFSISDYYDNGTAGDILVGVLFVLGFFLLSYKGPEPVDNIAADVGCVAA
                     LGVALFPTTYCTSCWVHTMHFIFALLLFSVFIFFSIYLFRKTNPAKKMTSEKKNRNRV
                     YLICGIIMILCIAAITFSLLVLDASVSAKYKLVYWFESIALVSFGISWITKSESFYLQ
                     DKEP"
     gene            1790..2548
                     /locus_tag="PLO70_08810"
     CDS             1790..2548
                     /locus_tag="PLO70_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74606.1"
                     /translation="MKKIILLLFIAFSITAFAQKTDLTTVNTVKPKLGQKMAFEAAYK
                     VHTAKFHKAEEKISVYEIMTGTYAGFYHLVNGGRSYADFDKARTDASTHSLDLDKTFF
                     PYLEETRNGIYRLVDSLSFHQEIQGESYLVNLRHIKASLQEDYRLESARTVKILSKLK
                     GTFWDNLSFNLFEQLWDGSEPVVVQIRNLKDGFKSLEPNYYGAATTGAPTFKDEYVKA
                     YGTADWDKRVKLLEDAVVKNEQYIMKLRKDLSSQ"
     gene            2878..4236
                     /gene="gdhA"
                     /locus_tag="PLO70_08815"
     CDS             2878..4236
                     /gene="gdhA"
                     /locus_tag="PLO70_08815"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQZ74607.1"
                     /translation="MKTSDTNAICKQFIEMVEQRSPGQPEFIQAVSEVAQFLVPFVEE
                     NPQYKKANILERIVEADRIIIFRVPWIDDQGNYRVNRGYRVQMNNAIGPYKGGLRFHP
                     SVNLGILKFLAFEQVFKNSLTTLPMGGGKGGSNFDPQGKSDGEVMRFCQSFMTELCRH
                     IGPDLDVPAGDIGVGAREIGYLFGQYKRIRNEFTGVLTGKGLAYGGSLIRPEATGYGV
                     IYFTEEMLKGINTSLKGKTVAISGFGNVSWGVVLKATELGAKVVTISGPDGYIYDKEG
                     ISGEKIDYLLEMRASGNNRAEMYREKFPNAEFHKGKRPWEQKVDIAIPSATQNELDEE
                     DARKLVANGVICVAEAANMPSTLDAIKVFLNAKVLFAPGKAANAGGVATSGLEMSQNA
                     IHLNWSREQVDNRLKEIMIGIHTQCSRYGKETDEYINYVKGANIAGFVKVADAMLAEG
                     HV"
     gene            complement(4417..6300)
                     /gene="htpG"
                     /locus_tag="PLO70_08820"
     CDS             complement(4417..6300)
                     /gene="htpG"
                     /locus_tag="PLO70_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQZ74608.1"
                     /translation="MVQEKGTISINTENIFPIIKKFLYSDNEIFLRELVSNAVDATQK
                     VKRLGSLGHYNKELGELKIKVATDATAKTITISDNGIGMTAEEIKKYINQVAFSGATE
                     FMEKFKEAKDANEIIGRFGLGFYSAFMVADKVEIQTLSYQDGAEPARWTCDGSTSFEI
                     TEGSRTERGTDVILYINDESKEFLEEHRISGILNKYAKFLPVPVQFGTRQESVPDGED
                     PEGKPQYKSIEVDNIINTTVPIWTKAPTDLKEEDYLDFYRELYPFSEEPLFWIHLNVD
                     YPFNLTGVLYFPKLKNDFEIQKNKIKLFSRQVFITDEVKDIVPEFLLLLHGVIDSPDI
                     PLNVSRSFLQADSNVKKINSYISRKVAEKLQELFKKDRKVYEEKWNDIGLFVKYGALS
                     DEKFYEKAKDFLLLTNTANEHFTLEEYSAKVKDFQTDKNGQLVYLYTIDAEHQHSYVQ
                     AANKKNFDVLVMNSPIDTHFIHHLEMKLEKTALKRVDADVLDKLIEKVDAVKHTLTEE
                     ETTKLKTVFEKAINNTNMKVEVESLPAEALPVTITIEEWMRRMKDMSKLGGGMNFYGN
                     MPDTYKVAVNANHKVVTKILQAEETQQTALAKQAFDLALLAQGMLKGADLTAFVERSV
                     EVL"
     gene            complement(6464..6868)
                     /locus_tag="PLO70_08825"
     CDS             complement(6464..6868)
                     /locus_tag="PLO70_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ74609.1"
                     /translation="MSNITQVVNEDKNLKTLKKGVHASDLDQLLSSTGPFTFFAPTDL
                     AFDKLEKGKMDKLLEPQNRAQLADLLNNHIVNGKIPYQDLKDGDKLTTVNGRELVVQV
                     KNGAAHIGDITILPRETRISNGVIHSMDTVMV"
     gene            complement(6949..7977)
                     /locus_tag="PLO70_08830"
     CDS             complement(6949..7977)
                     /locus_tag="PLO70_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQZ74610.1"
                     /translation="MSSITSFNNQIKQVMILFLLLLLIFLSIKELYIFFPGLLGALTF
                     YILSRSTYFQLIYHRKWKKGWTAGLYLLVYFLLLALLVYFIVILLEKQVRPFFNDPAA
                     ALGSAKQAINEAQQKAGFTFISDETLSNLQQKLSEFVPMLVNSTSNLVINLALLLFVL
                     YYMLVHGKEMESYLGRVIPLKQTNIDLLASETKRIVKASALGIPIISIIQGLTATAGY
                     IIFEVNEYVLWGFLTGVFAFFPVVGTMIIWVPLVIYMYASGNTWNATGLLLFSVIVTG
                     NVDYLARITLLKKLGNVHPMVTVLGVIVGLGLFGFIGLIFGPLLVNYIILLFRIYTNE
                     FIDHKTPG"
     gene            complement(8175..9374)
                     /locus_tag="PLO70_08835"
     CDS             complement(8175..9374)
                     /locus_tag="PLO70_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQZ74611.1"
                     /translation="MKTMFNKAFWLIIPVLAINACKKDKDVTPPVVPEQNKVAFITDN
                     ANRLYAANAQTGLNLWTYNTNTYYGYIYGSPAVYKNTVVFTDYENETVYCFNTETGAL
                     NWEKSSIELSWLCSPIIVNDVVYVGHSSYLLGLKLSDGSITIELPLYSSVNALNYSNG
                     LIIANTCGGHLYGIEPSGNIKWEYLSNYSCYHNNPAIANKIIYIVDAGGKFSAVNITT
                     GTEVWSKTINEYTENATPVYNNGMLFIASDYDNKMYAFDAANGTLKHTYTLSTGQYVN
                     GYMAPAILGEAVYITSEEGTLFAFNVTDESIKWQKALDLGGRSVKQRIPVKLDSRIED
                     YGFLSSVTIANDVIFIGAGKYLNAFDKEGNLKWQMATTDDIYCSSPVILSDKDKVYRA
                     GNAGIVE"
     gene            9716..10000
                     /locus_tag="PLO70_08840"
     CDS             9716..10000
                     /locus_tag="PLO70_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74612.1"
                     /translation="MQQQSNPFLYVSLLSIFVLLGLTGCSPGKEVSTASREEITLAIG
                     LDVWKFTALFMNPQYGRSRNVNGSYLVKFDRKKMEVYLPYLAVLMVGQMF"
     gene            9997..10218
                     /locus_tag="PLO70_08845"
     CDS             9997..10218
                     /locus_tag="PLO70_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HQZ74613.1"
                     /translation="MSGRSPLGFTTTDFSFEKEQDKKGAWVITIKPKDYSEVQIFIFT
                     LQGNGSAELNVQLTNRSPVSFRGIIDPVK"
     gene            complement(10309..10677)
                     /locus_tag="PLO70_08850"
     CDS             complement(10309..10677)
                     /locus_tag="PLO70_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HQZ74614.1"
                     /translation="MATKKKAAKKAAKKVAKKPVAKKAVKKAAKKKVAKKKPARKPNA
                     AFMKALTPSAALGAVVGTKALPRTEAVKKIWVYIKANKLQDKTNKRMINADAKLKVVF
                     GGKSQVSMFDMAKHLSKHLS"
     gene            10890..11546
                     /locus_tag="PLO70_08855"
     CDS             10890..11546
                     /locus_tag="PLO70_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQZ74615.1"
                     /translation="MFINIHSHQPPAAQEWCIQNLYNDFDKADLPGNYSIGIHPWHLD
                     AANWPQQIEALGDWCRHPHVLAIGECGLDKVCTTSFSLQQQVFSAQVQSANEIGKPMI
                     IHCVRAWEEVLHLLKQQRNRVPVIFHGFNKNKAIAQRIINEGYYVSFGKSLEKMAIRE
                     VLAALPPEKIFLETDNAAVTIESIYKWAADIFSIEINSLLLQIQKNAATVFGAALVQL
                     "
     gene            11543..12268
                     /locus_tag="PLO70_08860"
     CDS             11543..12268
                     /locus_tag="PLO70_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQZ74616.1"
                     /translation="MTTDTSWLSRTHLLIGEKALRKLTQSHVMVVGLGGVGSYAAEFI
                     ARSGIGKMTIIDGDVVDPSNRNRQLPALATNHGQSKAMIMAERLQAINPEIELHVIKE
                     FINPGMVMEHLTSKPCYIIDAIDSITPKITFIKMAYESGLPLVSSMGAGAKVDPTKLQ
                     VVDISKTYNCPFAQQIRKQLKKNNIRKGIKVVFSPEEPIKESLMLTDGKNFKKSAYGT
                     ISYLPAVFGAVAASVVIRDLIKK"
     gene            complement(12477..13814)
                     /locus_tag="PLO70_08865"
     CDS             complement(12477..13814)
                     /locus_tag="PLO70_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HQZ74617.1"
                     /translation="MRITYLKEDKGESIDLTRFIHDHSIDLNNTKDLDKLMDAIGDAK
                     YVLLGEASHGTHEYYTWRSVISRRLILEKGFSFIAVEGDWPDCYRVNRYVKGYTDAGS
                     SAKDVLSAFNRWPTWMWANWEIVALTEWMRKHNAAQPVNKRVGFYGLDVYSLWESLEA
                     IINYLDKTDKQAKEVAMQAWHCFEPYIEEEGQSYARATAHSWVPASCEAEVVDLLLEV
                     RNKMQQYNTDPEAVLNVERNAMIAVNAEKYYRAMMKGGPYSWNIRDHHMVDTLNKLMD
                     FHGIHAKAIVWEHNTHIGDARATDMRLDGMVNVGQLVNEMHYHDGVFAVGFGSYKGSV
                     IAGRHWNDVMRKMVVPPAIANSWEYIMHNAGATDRLMLMNKEVKDVLDKKEIGHRAIG
                     VVYNPQREYGNYVPSIMPLRYDAFIFFDETKALHPLHIEPDGQQMPETYPFGM"
     gene            14028..14714
                     /locus_tag="PLO70_08870"
     CDS             14028..14714
                     /locus_tag="PLO70_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQZ74618.1"
                     /translation="MKKEIREDVFIPVGSVTVEGHLGLPDKADSIVIFSHGSGSSRFS
                     PRNKYVADVLNEQNIATLLVDLLTTKEDLNYASRFDINLLTQRLVGVSKWVAEQPVFK
                     NFQIGYFGASTGAASALRAAATLEERVKAVVSRGGRPDLAMQVLPFVKAPTLLIIGSL
                     DDQVIELNQEAYDQLRCKKEMMIVEGASHLFEEPGKLQEVAVLAADWFKKYLVNTDSY
                     LDKAGIVLKN"
     gene            14720..15355
                     /locus_tag="PLO70_08875"
     CDS             14720..15355
                     /locus_tag="PLO70_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQZ74619.1"
                     /translation="MYFNDRYDAAMQLAPLLEKYKNEKGVILAVPRGGVPIGYYLAKY
                     LDFPLDLLMTKKLGHPGNEEFAIGAVSLEGSIVEETTGVPEQYIREETRRIREQLKER
                     YIKFMGNKEPVDIKGKIIIVVDDGVATGRTILSTIKILRNKEPRKIVVAVPVASEEAA
                     DRIREVVDDFICLHTPYPFYGVGRFYADFTQVEDEDVLLLLNELNTRGKVA"
     gene            15437..16036
                     /locus_tag="PLO70_08880"
     CDS             15437..16036
                     /locus_tag="PLO70_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQZ74620.1"
                     /translation="MNSPKHQLSYYVINGITLYRIVTAPLLLLLLFSGHYDLFKWLLG
                     VSFFTDLIDGYLARKFNVTSIMGTKLDSIGDDLTVAVAVIGLFVIFPDFIRQEKWVFI
                     ILLVFFLLQTVSAFIRYGRMTNFHTWLAKTAALLQGVFLQLTFFMGKPFLPLFYTAVT
                     ITLLELAEEIILVYILPVWEANIKGLYWVLKRKKPADTK"
     gene            16260..16751
                     /locus_tag="PLO70_08885"
     CDS             16260..16751
                     /locus_tag="PLO70_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HQZ74621.1"
                     /translation="MSKRNKTFMVKANEFVFSFDQEEIDKADFIKKNPAEFNLIKDNR
                     SVNAKLLESDIAGKNLKIEVDGETFELVIKDELDQRLDSMGFSSTSTKHIKEIKAPMP
                     GLVLEVAVKEGQEVKEGERILILEAMKMENSIMIHTTAIIKRIAVTPGQAVDKGQVLV
                     ELE"
     gene            16774..18249
                     /locus_tag="PLO70_08890"
     CDS             16774..18249
                     /locus_tag="PLO70_08890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQZ74622.1"
                     /translation="MKKILVANRGEIALRIMRTIRKMGIKSVAVYSDADRHSPHVLFA
                     DEAVCLGPAPSSQSYLNGDKIIAFCKELGVDGIHPGYGFLSENAEFAQKVTDAGIEFI
                     GPTPEAMRIMGSKLAAKESVKKYNIPMVPGIDRAIDDVNLAIEIADKIGYPVLIKASA
                     GGGGKGMRIVEKADDMQEQMERAISEAKSSFGDGSVFLEKYVTSPRHIEVQVVADKYG
                     NVIHGLERECSIQRRHQKVIEETPSAIVTPEMREKIGDAAVMVAKSCNYVSTGTVEFL
                     VDDKLNFYFLEMNTRLQVEHPVSEMIIGLDLVEQQIRIARGEKLGITQDDIKPNGHAI
                     ELRVYAEDPLNNFLPSIGTLTKYEKPTGEGIRVDDGYEEGMTIPIYYDPMIAKLVVHG
                     KTRTEAIQKMLQAIKEYKIEGVATTLPFGTFVFEHDAFFSGKFDTHFVKNYYTPEKIK
                     AKQKANAEIAAIVAIKYWQERKRMLIPVEHKSTSWKRRLAH"
     gene            18295..19836
                     /locus_tag="PLO70_08895"
     CDS             18295..19836
                     /locus_tag="PLO70_08895"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQZ74623.1"
                     /translation="MKPKVEILREKIEEGKLGGGVKRIEAQHKKGKLTARERVELLMD
                     PGSFEEIGALVTHRTKDFGMENQKYYGDGVITGFGTVNGRLVYVFAQDFTVFGGALSE
                     THAEKICRIMDMAMKSGAPLVGLNDSGGARIQEGVRSLGGYADIFYRNVQASGVIPQI
                     SAIMGPCAGGAVYSPAMTDFTIMVENTSYMFVTGPNVVKTVTNEEVSSEELGGASTHS
                     TKSGVTHLTAVNDMDCITQVKKLLSYMPQNCEDVVMKQPYTLGDETREVLETIVPASA
                     SQPYDIRSVIEGICDTDSFFEVHKEYADNIVVGFARLAGRSLGIVANQPMSLAGVLDI
                     ASSKKGARFTRFCDCFNIPLLVLVDVPGFLPGTDQEWNGIITNGAKLLYALSEATVPR
                     CTVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGASEIIFKNEIAAAEDPA
                     KKLLEKEAEYAELFANPYTAAERGFIDEVIEPRETRKKLIKAFAMLENKVAKRPRKKH
                     GNIPL"
     gene            complement(19845..20186)
                     /locus_tag="PLO70_08900"
     CDS             complement(19845..20186)
                     /locus_tag="PLO70_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74624.1"
                     /translation="MQQHKLLLWTGRIGGILVTAFFLSFFIGEGLPDIMNGMGKELLH
                     FLPFTLLTIAGFIIAWFQPLWGGRLLMAGAVLMAVYFLSNGDTRMALVYGLPSLLIGL
                     CFLGAVNKELI"
     gene            complement(20223..>20429)
                     /locus_tag="PLO70_08905"
     CDS             complement(20223..>20429)
                     /locus_tag="PLO70_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74625.1"
                     /translation="YTEDTKYFWETNLYDLATNDLVYSAQSQSFDPSSSESLGHEYGQ
                     MIATDLVKQHVLVNLKEQPVLKAM"
BASE COUNT         5927 a         4032 c         4472 g         5998 t
ORIGIN      
        1 ccagtgcttt ttttatagcg gctcccatct tttgctggct gtcaggattg agcagcagct
       61 cacgactgac ggcattatca ccaatctcga gtaatacacg ataaggagcg gtgttaatat
      121 gctcatcaag gctgtaaaaa cttagtttgc cggtggcctg gcaacggtaa tcgtttctgc
      181 ccgttgtact gtcgagtagt acaccacggt tctctaaccc gcttacttca ctgatagctt
      241 ttatcagtaa tgcagccagt tcacggttat ctttttcata tttatcaccg taatgaatat
      301 agccccatac agcattacgc ttcgtgccgc cattattatg tacagaaata aaaatagacg
      361 gtttcagttt attggatgct tgtaattcaa aggcataacc ggagatcttg ccgtcaataa
      421 cagcgctggt atgcaatatt ttggtattgc tgcctgagtc ggcatgatgg tattccgttt
      481 taccaagact ccatacttta tattctttta accgtggatt tgttttcatc gctgcttcag
      541 caatggcatt acaaaccgct gcttcactga aatctttacc ggtatctgtt tggtgggagg
      601 gctgccagac aacaactgtt ttttttctgc cggcataaaa tttatcactg catgattgta
      661 atactaaaaa gcataaagca aaagcaattg attttttagg gttcataaca aatatctttt
      721 ccggaagtga atgaaacagg atgtcacagg aaagttaaac agaatacaga agcgaaatta
      781 aagcaggacg aaatttatat ctaacctgtc ttttgcaaaa gctggttgtc acttaaaggt
      841 ctgaacccgg aatatgctta cattcatatt catttattaa acctcctgta aaccttagcc
      901 tatgcacccg gatcaatctg acagtaccgc cagacacaaa gaaccgaatg accagatcat
      961 ttcgtattat accttgcggg tcctcatagg cgccaccggt gttttgcttc ccctgttagt
     1021 gttttttgga aaatggattt cagaaagttc aggtgtcctg gaattttcca tcagtgatta
     1081 ttatgataac ggtaccgccg gtgatatact ggtgggtgta ttgtttgtgc tggggttttt
     1141 cctgctatct tataagggcc cggaaccggt tgacaacata gccgctgatg tgggatgtgt
     1201 ggctgcattg ggggtggcat tatttcctac cacttattgt acttcctgct gggtgcatac
     1261 gatgcatttt atttttgcct tactgttatt ctctgtattc atttttttct ccatttatct
     1321 tttccgtaaa acaaaccctg ccaaaaaaat gacgtcagaa aagaaaaatc ggaaccgggt
     1381 ttatcttatc tgcggtatta ttatgatact ttgtattgct gctattacat tcagtttatt
     1441 agtgctggat gcctctgtga gtgcgaaata taaactggtg tattggtttg aatccatcgc
     1501 cctggtttcc tttggtattt cctggatcac caagtcggag tctttttacc tgcaggataa
     1561 agaaccgtag gggaacccgg taaaaggaag accccttctg gtaataaatt ctttttttct
     1621 ttatttggtt aattaggtta acttactata gtcttaattc ctccccctgt aattcctttc
     1681 ccggtaatac cctcccgaat agtgtgtatt attttttatg aatgaagtac atgcagttga
     1741 tttttcaatt gcagcactga taattatttt ttaaccaaaa caaccacaca tgaaaaagat
     1801 cattttgttg ttatttattg cctttagtat tacagccttt gctcaaaaga ccgacctgac
     1861 taccgttaac accgtgaaac cgaagttggg ccagaagatg gcatttgaag cggcgtataa
     1921 agtacatacc gccaagtttc acaaggctga agaaaaaatt tctgtgtatg aaatcatgac
     1981 cggcacttat gcaggatttt atcatttggt aaatggtggg aggtcatatg cggattttga
     2041 taaagcaaga actgatgcat ccacccacag cctggatctt gataagacat tctttcctta
     2101 cctggaagaa acaaggaatg ggatttaccg cctggtagat agtttaagtt ttcaccagga
     2161 aatacaagga gaaagctatt tggtcaatct gaggcatatc aaagcctctc ttcaggaaga
     2221 ttatcgcctg gaatctgcaa gaactgtaaa aatattaagt aagttaaaag gaacattctg
     2281 ggacaatctt agctttaatc tctttgaaca gttatgggat gggtctgaac ctgttgtggt
     2341 acaaatacgt aatcttaaag acggattcaa atcgcttgaa cctaattatt atggagctgc
     2401 aactaccggt gcacctactt ttaaagatga atatgtaaaa gcatatggaa ctgctgactg
     2461 ggataaaagg gtcaaactgc tggaagatgc tgttgttaaa aatgaacagt atattatgaa
     2521 attgagaaaa gatcttagct cacagtagac tactataata ttgattaaaa gatcccgggc
     2581 aggtctgggg tctttttttg tattgtacat ccccgttagt ggatcgtata gcaggccctg
     2641 caacacttcg aggaatttca attatgcaga acttttgaat agtcaactgg tattcaattt
     2701 cgccaaccct aaacggcccc ttttgactgg ttgtcaatac tcatatacaa atggcttttt
     2761 tgctgacttt actcatactt accaatgtta ctgacttttt acttttacat gatgccggag
     2821 atagtttttc agctatcaaa acccgcaaaa aatattagtt aacaacaaaa ccgctgtatg
     2881 aaaacatcag atacaaatgc catctgcaag caattcatcg agatggtgga acaacgcagt
     2941 cccggtcaac ccgaatttat acaggcagtt tctgaagtag cacaattcct ggttccattt
     3001 gtagaggaaa atcctcagta taaaaaggcg aatattctgg aaaggattgt ggaagctgac
     3061 cgcatcatca ttttccgggt accctggata gacgaccagg gtaattatcg tgtaaaccgg
     3121 ggttaccgtg tacaaatgaa taatgcgatt ggcccttaca agggtgggct tcgtttccat
     3181 cccagtgtaa acctgggcat ccttaagttc cttgcctttg aacaggtatt taaaaattct
     3241 ctcaccacgc tgccaatggg cggcggaaaa ggtggttcta attttgaccc acagggaaaa
     3301 tcggatggag aagttatgcg cttttgccag tcatttatga ctgaattatg ccggcatatt
     3361 ggtcctgacc ttgatgtacc agccggtgat attggtgttg gcgcaagaga aataggttat
     3421 ctgttcgggc aatataaaag aatcaggaat gaatttaccg gtgtattaac cggaaagggg
     3481 cttgcgtatg gtgggtcttt aataagaccc gaagccaccg gatatggtgt tatttatttt
     3541 accgaggaaa tgctaaaagg aattaataca agcttaaaag gtaagaccgt agccatatcg
     3601 ggctttggaa atgtttcctg gggtgttgta ttaaaagcaa ctgaactggg ggcaaaagta
     3661 gttaccattt ccgggcctga cggttatatc tatgataagg agggtattag tggcgaaaag
     3721 atagattacc tgctcgaaat gagggcaagt ggtaataaca gggctgaaat gtatagagaa
     3781 aaattcccga atgccgaatt tcataaaggc aaaaggccct gggaacaaaa agttgacatt
     3841 gccatacctt ctgctacgca gaatgaactg gatgaagagg atgcaaggaa actggtggcg
     3901 aacggagtaa tttgtgtggc agaagcagca aatatgccaa gtacattgga cgccatcaaa
     3961 gttttcctga atgccaaagt gctgttcgct cccggcaaag cagcaaatgc agggggtgtt
     4021 gccacatcag gccttgagat gtcgcaaaat gccattcatt taaactggag cagagaacaa
     4081 gtggataata ggctgaaaga aatcatgatt ggaatacata cacaatgctc cagatacggc
     4141 aaggaaactg atgagtatat aaactatgtc aaaggcgcaa atattgccgg atttgtgaag
     4201 gtagctgacg ccatgctggc agaaggccat gtgtaaaggc cagcaatttg caatagccgg
     4261 atattgttta acagttttgt attccggttt taagaagaac gctaagaaaa agccccgaaa
     4321 aattccgggg cttttttaga actattcata catatgcttc tttcaataag tataaaaagt
     4381 cggacagaaa taaatagaac cagcaggtag gtacaattac agtacttcca cacttctttc
     4441 tacaaaggct gtcaggtctg cgcctttcag catgccttgt gccagcagtg ccaggtcaaa
     4501 agcctgtttg gccaatgccg tttgctgtgt ttcttctgcc tgtaatattt tagtcaccac
     4561 tttatgattg gcattcacag ccactttata ggtgtcgggc atattgccat aaaaattcat
     4621 acccccaccc agtttactca tgtctttcat gcggcgcatc cattcctcaa tggtaatggt
     4681 caccggtaaa gcttcagcgg gaaggctttc cacttccact ttcatattag tgttgttgat
     4741 ggctttttca aaaacagttt tcagtttagt agtttcctct tcagtcaatg tatgttttac
     4801 tgcatctact ttttcaatca gtttatccaa cacatctgca tccactctct tcaacgctgt
     4861 tttttccagt ttcatttcca ggtgatgaat aaaatgcgtg tcgatcgggg agttcatcac
     4921 caagacgtca aaattctttt tattcgctgc ctgcacataa ctatgctggt gctctgcatc
     4981 aatggtatag agatagacca gctgcccgtt tttatctgtt tgaaaatctt ttactttggc
     5041 actgtactct tccagcgtaa aatgttcgtt ggcggtattg gtgagtaata aaaaatcttt
     5101 tgccttttca tagaattttt catcagaaag agcgccatat tttacgaaca ggccaatatc
     5161 gttccatttt tcttcatata ctttgcggtc ttttttgaaa agctcctgca gtttttcggc
     5221 tactttgcgg gagatatagc tgttgatctt ttttacatta ctgtcggcct gcaaaaaaga
     5281 acggcttaca ttcaatggga tatcgggaga gtcgatcaca ccatggagca acaataaaaa
     5341 ttccggcacg atatctttta cctcgtctgt gataaatacc tgcctgctga aaagtttgat
     5401 cttattcttc tgtatctcaa aatcattttt cagttttgga aaatatagaa cgccggtcag
     5461 gttaaacgga tagtccacat tcaggtgtat ccagaacaag ggctcttctg aaaaggggta
     5521 gagttcccgg taaaaatcaa gataatcttc ctctttcagg tcggtagggg ccttagtcca
     5581 gatcgggaca gtagtattga tgatattatc aacctcaatg cttttgtatt gtggtttacc
     5641 ttccggatct tctccgtcgg gtacggattc ctgcctggtc ccgaattgta caggtaccgg
     5701 caggaactta gcatatttat tcaggattcc gctgatgcga tgttcttcaa gaaactcttt
     5761 tgattcatca ttgatataaa gaatgacatc cgtacctctt tctgtacggc tgccttcggt
     5821 aatttcgaaa gaagtgctgc catcgcaggt ccagcgggcg ggttcggcgc catcctggta
     5881 ggataaggtt tggatctcta ctttatcagc aaccataaat gcggaataaa agcccaggcc
     5941 aaaacgaccg atgatctcat tcgcatcttt tgcttctttg aacttttcca tgaattccgt
     6001 agccccggaa aatgccacct ggttgatgta tttttttatt tcttcagcag tcatgccgat
     6061 accattgtca gagatcgtga tggttttggc agtggcatct gtggctactt ttattttcag
     6121 ttcgcccagt tctttgttat agtgtccaag ggaaccaagc ctttttactt tttgagtagc
     6181 gtccacggca ttactgacca attcccgtaa aaagatttca ttatcagagt ataaaaactt
     6241 cttgatgata gggaagatgt tctcagtgtt gatggagatt gttccttttt cctgtaccat
     6301 tttatttagt gtttatcaat ttgtttaaaa gaatagttgc caggtagcaa gcctcgttcc
     6361 aaagcggtct taattgccag attgacagcg gtaataagtc cctgcccgga cataaaaatg
     6421 ccacctggtt aaccggtggc atttaaagta ttttaaccag tgattaaacc attaccgtat
     6481 ccatcgaatg tatgactccg ttcgatatcc gggtttcacg gggcaggatt gtaatgtcgc
     6541 caatatgagc cgcgccattt tttacctgta ccaccaactc cctgccattt accgttgtca
     6601 gcttatcgcc gtctttcagg tcctggtagg gtatctttcc gttaacaata tgattattca
     6661 gcagatcagc cagctgtgcc ctgttttgag gctccaggag cttatccatt ttcccttttt
     6721 ctaatttatc gaaagccagg tctgtaggag caaaaaaagt aaatgggcct gtactgctca
     6781 gcaactggtc caggtcggaa gcatgcacac ctttctttaa tgttttcagg tttttatctt
     6841 cattcaccac ctgagtaata tttgacatat atgtttattt atccaccggc agtattaccg
     6901 gcaaggcggg gaaggtagtc attagcaggc agctttactg ttaatgtttc agccgggtgt
     6961 tttgtggtca ataaactcat tggtataaat tctgaaaaga aggatgatat aattgaccag
     7021 caacggccca aaaatgagac cgataaagcc aaataatccc aaaccaacga tcactcccag
     7081 tacggtaacc atcggatgta catttcctaa ttttttcaat aacgtaatgc gggccaggta
     7141 atcaacattt ccggtaacaa taacactaaa caatagtaac ccggtagcat tccaggtgtt
     7201 accactggca tacatgtaaa tgaccagcgg gacccagatg atcatagtgc ccactaccgg
     7261 gaaaaaagca aatacaccgg tgaggaaccc ccacagcaca tattcattca cttcaaaaat
     7321 gatatacccg gcagtagctg ttaatccctg tataatagaa ataataggaa ttccaagggc
     7381 actggctttt acaattcgtt ttgtttcgga ggcgaggaga tcaatattcg tttgctttaa
     7441 agggatgacc ctgccaaggt atgattccat ttccttgcca tgcaccagca tataatacaa
     7501 aacaaaaagc agcagggcaa ggttaattac gaggttggag gtgctattca ccagcatagg
     7561 cacaaactcc gacaattttt gctgcaggtt ggatagtgtt tcgtctgaaa taaatgtgaa
     7621 gcctgctttt tgttgtgcct cgttgatagc ttgctttgcg ctgccaagtg ccgctgccgg
     7681 atcattaaaa aatggacgca cctgtttttc cagcagtatt acaatgaaat aaacaagtaa
     7741 tgccagtaac aggaaataaa ccagcaggta caatcctgct gtccacccct ttttccattt
     7801 acggtgatag atcagttgaa aataagtact gcggctaaga atataaaagg ttaatgcccc
     7861 cagtaatccc ggaaagaata tataaagttc tttgatggac aggaaaatca acaacagcag
     7921 caggaataga atcattacct gcttgatctg gttattgaaa ctggttattg atgacatagt
     7981 ttcttgtgta agtagcagca ctcacataca tacttagaag taaggtaaaa ctttttttaa
     8041 tgaccagtta tgacagttgt tagttcctgc cagtttgaaa gtcgtatatc tgtcacaggg
     8101 ttgcaataat ctgtttcttt actaataaaa agccccgggt tagccggggc cttcaatctt
     8161 tgcaatactg aaacttactc gacaataccg gcattacctg cacggtaaac tttatcctta
     8221 tcggataaaa taacaggaga actgcaatag atatcgtcag tagtagccat ctgccatttt
     8281 aagttacctt ctttatcaaa ggcatttaaa tatttacctg ctccgataaa tatcacatca
     8341 ttggcaatcg ttactgatga taaaaagccg taatcttcta tcctgctatc cagcttaaca
     8401 ggtattctct gttttacgct tcttccgcca aggtcaagtg ctttttgcca tttaatagat
     8461 tcatctgtta cattaaaggc aaacagtgtt ccctcttcag aagttatata aacagcttca
     8521 cccaggatgg ccggtgccat atacccgttt acatattgac ctgttgataa tgtataggta
     8581 tgtttcagtg tgccattggc agcatcgaac gcatacattt tattatcata atcgctggct
     8641 atgaataaca ttccattgtt ataaactggt gtagcatttt ctgtgtactc attgattgtt
     8701 ttactccaca cctctgtacc ggttgtaatg ttaactgcag aaaactttcc gcccgcatcg
     8761 actatgtaga tgatcttatt cgcaatagcc gggttattgt ggtaacagga ataattagac
     8821 aggtattccc attttatgtt accggagggt tcaataccat ataagtgacc accgcaggta
     8881 ttggcaataa ttaagccatt ggaatagttc aatgcattta cggaactata cagcggtaat
     8941 tcgatcgtta tagaaccatc actgagtttc aatcccagca gataggagga atggccaaca
     9001 taaacaacat catttacgat gatcggggag caaagccatg acaactcaat agatgatttt
     9061 tcccagttca atgcccctgt ttctgtattg aaacaataaa ctgtttcatt ttcataatcg
     9121 gtaaatacta ccgtattctt gtatacagcc ggtgaaccat agatataacc ataataggta
     9181 ttggtgttat aggtccaaag gttgagccct gtctgggcat tagcagcata aagcctgttg
     9241 gcattatcag taataaaagc cactttattt tgctctggta caacaggagg ggttacatct
     9301 ttatcttttt tgcaagcatt aatagccaat actggtatta tcagccaaaa cgctttatta
     9361 aacatcgttt tcataaaact agttttaaag gttggcttaa taaataattc aatacgttat
     9421 tggcgtattt aagtttatta agttttaaaa ttatttgtct tgttgtggat tttccgtcaa
     9481 agattgattc ggtaaacgct gcccagcaat ccccgggtca gtgtatattc atttatacat
     9541 aaccgtgact tgtactatga agttaagaat gactatacac tttttccaaa ttttaaaggc
     9601 tgatttcagg attataatat tatgattcta atcataacag ttagcttttt tcctgtatgg
     9661 cggacaggaa attgtttgaa agcacttgtt aatttcaggt ataaaagcct gatttatgca
     9721 acaacagtca aatccctttc tttatgtttc attgctgtca atctttgttt tgttaggcct
     9781 aacaggttgt tctcccggta aggaggtttc aacggcttcc agggaagaga tcactcttgc
     9841 gattggtttg gatgtatgga aatttacagc actttttatg aacccgcaat acggccgatc
     9901 cagaaacgta aatggcagtt acctggttaa gtttgacagg aaaaagatgg aagtatacct
     9961 tccgtatttg gccgtgctta tggtgggtca gatgttttga gtggccggag tccgctgggc
    10021 tttacaacca ctgatttcag ttttgaaaaa gaacaggata aaaaaggtgc ctgggtaatt
    10081 acaatcaaac cgaaagatta tagtgaggta caaattttca ttttcactct gcagggtaat
    10141 ggcagtgctg aattgaatgt acagctgacc aaccggtcgc ctgtaagttt caggggaatc
    10201 atcgacccgg taaaataagg aatacagttc ctggtagttg acactgcttt tattattaaa
    10261 ataaaaaagg cttgccatgg caagcctttt ttcaagaggt catttttctt aactcaaatg
    10321 tttagacagg tgttttgcca tatcaaacat agatacctgg cttttaccac cgaaaacaac
    10381 tttcagtttg gcatccgcat tgatcatacg tttgttggtc ttgtcctgca gcttattcgc
    10441 cttgatgtaa acccatatct ttttcactgc ttctgtacgg ggcaatgctt ttgttccgac
    10501 tacggcacct aatgctgcac ttggagtaag cgctttcata aatgctgcgt ttggcttacg
    10561 ggcaggtttc tttttagcaa cttttttctt agctgctttt tttactgctt ttttagctac
    10621 tggcttttta gccactttct ttgcagcttt tttagctgct tttttctttg ttgccatgtt
    10681 ttttagattt agaattttaa tgggtattca gttataccgg tatcaaatat agattatttc
    10741 tactctcggt gtaactatga aaaaaaaatc tggccttatt ttttggttgt ttatccacag
    10801 ttgtactgta tacaggtaga atacagggaa gagaacttgc tgtttttatt cataggcagt
    10861 tcctaaattg tgttataaaa gatcattttt tgttcatcaa tattcatagc catcagccac
    10921 ctgctgcaca ggaatggtgc attcaaaacc tgtacaatga ctttgataag gcagatttac
    10981 cggggaacta ttccattggc atacatccct ggcatttaga tgctgcgaat tggccgcagc
    11041 aaatagaagc attgggggat tggtgccgtc acccccatgt gctggctatt ggtgaatgtg
    11101 ggttggacaa ggtttgcaca accagttttt cactgcagca gcaagtgttt tcggcccagg
    11161 tacaatctgc caacgagata ggtaaaccaa tgattattca ttgtgttcgg gcatgggaag
    11221 aagtgctgca tttgttgaaa caacaacgaa acagggtgcc ggttatcttt catggattta
    11281 ataaaaacaa ggcgattgcg caacgaatca tcaatgaggg gtactatgta tcatttggta
    11341 aatcattgga aaaaatggcg atacgggaag tgctggcggc cttgccgccg gaaaaaattt
    11401 tcctggaaac ggataatgcc gctgtaacga tagagagtat ttacaaatgg gctgcagaca
    11461 ttttttcaat tgagatcaat tcacttcttt tgcaaattca aaaaaatgca gctactgttt
    11521 ttggcgctgc tcttgttcaa ctatgacgac tgatacttcc tggctttcca ggacacattt
    11581 actgatagga gaaaaagcac tgcgcaaact tacccaatca catgtaatgg tagtgggcct
    11641 gggtggtgtg ggctcttatg ctgctgaatt cattgcccgt agtggcattg gtaagatgac
    11701 cattattgat ggcgatgtag tggaccccag caaccgtaac cggcagttac cggcattggc
    11761 caccaatcac ggtcaatcaa aagcgatgat aatggccgag cggttacagg ctatcaatcc
    11821 tgaaattgaa ctgcatgtca tcaaagaatt tatcaatccc ggaatggtga tggagcatct
    11881 tacttctaaa ccctgctata tcattgatgc cattgacagt ataaccccta aaatcacctt
    11941 tatcaaaatg gcgtatgaaa gtggtttgcc tttagtcagt agtatgggtg caggagctaa
    12001 agtggatcct actaaactgc aggtggttga tatttcaaaa acatataact gtccttttgc
    12061 acagcaaatc aggaaacagc ttaagaaaaa taatatccga aagggtataa aagtggtttt
    12121 ttcaccggaa gagcccatta aagaaagcct gatgttaact gatggtaaga acttcaaaaa
    12181 atctgcatac ggcaccattt cttatttgcc tgcagtattc ggtgctgtgg ccgcttcggt
    12241 ggtgatcagg gatttaatta aaaaatgaaa ggaatcaatt cagcgatctt tctttttgga
    12301 aaataaaaaa atgatcaggg tgatgatgac caggctgagc gaaccatata ccacataact
    12361 gaaaggcatg gagaaagatt gtatatgaaa gtaaactttt ccgtttttac agcaataaac
    12421 ctcccatcaa aaaatgatac tgctggccat atccattcgc atgccaggtg aaccagtcac
    12481 ataccaaaag gataggtttc cggcatctgt tgcccgtcag gttctatgtg caaaggatgt
    12541 aaggcctttg tttcatcaaa gaagataaat gcatcatacc gcaacggcat gatgctgggt
    12601 acataattgc catattcccg ttgaggatta tagacaactc caatggcacg atgaccaatt
    12661 tcttttttat ccaacacatc tttcacctct ttattcatca gcatcagccg gtctgtggcc
    12721 cctgcattat gcataatgta ttcccagcta ttggctatgg ccggtggaac aaccatctta
    12781 cgcatcacat cattccagtg acgaccggct attacagatc ctttgtaaga tccaaagcct
    12841 acggcaaata ctccatcatg ataatgcatt tcattcacca gttgtcccac atttaccata
    12901 ccatccagtc gcatatcagt agccctggca tcaccaatat gagtattatg ttcccataca
    12961 atggctttgg catgtatacc atgaaaatcc atcagcttgt ttaaagtatc aaccatgtga
    13021 tgatcccgga tattccagct atagggtccg cctttcatca tcgcccgata gtatttctct
    13081 gcatttactg caatcatagc attgcgttct acattcagca cggcttcggg atcagtattg
    13141 tattgctgca ttttattccg gacctccagc agcaggtcca ccacttctgc ttcacaggat
    13201 gccggcaccc acgaatgggc tgttgccctt gcataagatt gtccctcttc ctcaatataa
    13261 ggttcgaagc aatgccaggc ctgcatagcc acttcttttg cttgcttgtc tgttttatcc
    13321 aggtaattga taatggcttc caggctctcc cacaaactgt aaacatccaa cccatagaag
    13381 ccaacccgtt tgtttaccgg ctgtgctgcg ttatgctttc gcatccattc agtcagtgcc
    13441 actatttccc aattggccca catccaggta ggccagcggt tgaatgcaga cagaacgtct
    13501 ttggcactgc tgcctgcatc agtataacct tttacatacc ggtttacccg gtaacagtcc
    13561 ggccagtcac cttctacggc aataaaagag aatccttttt ccagtatcag ccgcctgctg
    13621 atcactgacc gccaggtata atattcatgt gtgccgtggg acgcttcacc cagtaacaca
    13681 tatttcgcat ccccgatggc atccatcaac ttatcaagat cctttgtatt gttaagatca
    13741 atggaatgat catggatgaa tcttgtaagg tctatacttt cacccttatc ttcttttaaa
    13801 taggtgatac gcatataatt caatttaatt taataattca agtaacaata attcactccc
    13861 tgaacctatg ataaggttca ttcatttata tgagtgaact caccgaaaac ggcgatgatg
    13921 cctatgtaaa ttcacaacat gaaataaagt gcaggtatat gtttatagcc tgctttttgt
    13981 aagtgaatga ttgatatttt tttatcaggc ggtagaagtg tataaagatg aaaaaagaga
    14041 ttcgggaaga tgtgtttatt ccggtaggtt ctgtcacggt tgaagggcat ctgggtttgc
    14101 ctgataaagc ggatagtatt gttatctttt ctcatggtag cggcagcagc cgttttagtc
    14161 cccgcaataa gtatgtagct gatgttttaa atgagcaaaa tattgccacg ctgttagttg
    14221 atctgctcac aacgaaagag gatttgaatt atgcttcacg gttcgatatt aatttactta
    14281 cccaacggtt ggtgggtgtt tccaaatggg tagcagagca accggttttt aaaaattttc
    14341 aaataggtta cttcggggcc agtacgggtg ctgccagtgc attaagagct gcggctacac
    14401 tggaagagag agttaaagca gttgtgtcaa gaggtggccg gccggacctg gccatgcagg
    14461 tactgccatt tgtaaaagct cccactttgt taattattgg ttcattggat gaccaggtga
    14521 ttgaattgaa ccaggaagca tatgaccagt tacgatgcaa aaaagaaatg atgattgtag
    14581 aaggcgcttc tcatttgttt gaggaacccg gcaagctgca ggaggtggcc gtattagctg
    14641 cggattggtt taaaaaatac ctggtgaata ctgattctta tcttgataaa gccggtattg
    14701 tgcttaaaaa ttaagctata tgtatttcaa cgaccgatat gatgccgcca tgcaactagc
    14761 tccgcttctg gagaagtata aaaatgaaaa aggtgttatc ctggctgtgc cgagaggtgg
    14821 agtaccaatt ggttattacc tggccaaata ccttgacttt ccgttggatc tgctgatgac
    14881 caaaaaatta ggacatcccg gtaatgaaga atttgccatt ggtgcggtaa gccttgaagg
    14941 aagtattgta gaggaaacca cgggtgtacc ggaacaatat atcagggagg aaacccggcg
    15001 tatcagggag cagctgaaag aacggtacat aaaattcatg ggtaataaag agccagttga
    15061 tataaaggga aaaataatca ttgtggtgga tgatggtgtg gcaacgggac gaactatact
    15121 atcgacaata aaaatccttc gcaataaaga accccgaaag attgtggtgg ctgtcccggt
    15181 tgcatctgaa gaagccgcgg atagaattag agaagtggtg gatgatttta tctgcctgca
    15241 tacaccttat ccattttatg gtgtgggcag gttttatgcc gattttacac aggtggaaga
    15301 tgaagatgtc ctgctgttgt tgaatgaatt aaacaccagg ggaaaagtgg catagtcata
    15361 gttgctgtta gggcaaagcc ttattttcgg ttgttggtta atctcccagg tttacaacaa
    15421 ctcaattata ttatttgtga acagccccaa acaccaattg agctattatg tcatcaatgg
    15481 tattaccctg tacagaattg ttacagctcc tcttttatta cttctcctct ttagtggtca
    15541 ttatgattta ttcaaatggt tgcttggagt tagttttttc actgatttaa ttgatggtta
    15601 cctggcgaga aaattcaacg taaccagtat aatgggcaca aagctggatt ctatcgggga
    15661 tgatttaact gttgccgttg cagtgatcgg tttatttgtc atttttccgg actttatcag
    15721 gcaggagaaa tgggtgttta tcattctcct tgtttttttt ctgcttcaga ctgtctctgc
    15781 ttttatccgg tatggcagaa tgactaattt tcatacctgg ctggccaaga cagcagcttt
    15841 gctgcagggt gtattcctgc agttgacttt ctttatgggt aaaccatttt tgcctttgtt
    15901 ttatacagcc gtcaccatta cactactgga actggcagaa gaaatcatat tggtttatat
    15961 tttaccagta tgggaggcga atataaaagg attgtattgg gtattgaaga gaaaaaaacc
    16021 ggctgacaca aagtaaccgg ccggctgctt acagtcgcgg gtgacattta tttggtgaat
    16081 caatgagtat aatcattatt tatgatggca taaaccgcaa tcttgcatgc tgaactgata
    16141 agttacaagc actaaccttt tccaatatct gcaaaatatc cccataccgg gttcagcgat
    16201 gatgaaacag ctttcaataa ttccggaatc aattaactta caagtaaatc ccttgctata
    16261 tgtcgaaacg taataaaacc tttatggtta aagcaaatga gtttgttttc tcttttgacc
    16321 aggaggagat cgataaggca gactttataa aaaaaaatcc agcggaattt aatcttatta
    16381 aagacaaccg ctccgtaaat gccaaactac tggaatcaga tattgccggt aaaaatttaa
    16441 agatcgaagt ggatggagaa actttcgaac tggtaataaa agatgaactg gaccagcggc
    16501 tggacagtat gggattcagc agtacttcca ccaaacatat caaagaaata aaagctccta
    16561 tgcccggcct ggtgctggag gtggctgtga aagagggcca ggaagtaaaa gaaggtgaaa
    16621 ggatactgat tctggaagct atgaagatgg agaacagtat catgatacat actactgcaa
    16681 taatcaaaag aatagctgta acaccgggac aggcggtgga caaggggcag gtgctggtag
    16741 agctggaata attcaacaat caaaatcaat cagatgaaaa aaatattagt tgccaaccgg
    16801 ggagaaattg cgttgcgtat catgcggacc atccggaaga tgggaataaa atcagtagcg
    16861 gtatactctg atgcagaccg tcattctccc catgtgttat ttgctgatga agctgtttgt
    16921 ttaggtccgg ctccgtccag tcaatcctac ctgaatggag ataagatcat tgcattctgt
    16981 aaagaacttg gtgtggatgg aattcacccg ggctatggtt tcctgagtga gaatgcagag
    17041 tttgcacaga aagtaacaga tgccggcatt gaatttattg gacctacgcc tgaagcaatg
    17101 cgaatcatgg gttcaaagct ggccgcaaag gaaagtgtaa aaaaatataa tatcccgatg
    17161 gtacccggta ttgacagggc cattgacgat gtgaacctgg caattgaaat agcagacaaa
    17221 attggttacc cggtattgat aaaagcttct gcaggtggtg gtggtaaggg aatgcggatc
    17281 gttgagaaag ctgatgatat gcaggaacaa atggaaaggg ctatcagcga agcaaaatct
    17341 tcttttgggg atggctcagt attcctggaa aaatatgtta cctcaccccg gcatatagaa
    17401 gtgcaggtgg tggccgacaa gtatggtaat gtaattcatg gcctggagag ggaatgtagt
    17461 atccagcggc gtcaccagaa agtaattgag gaaacacctt ctgctattgt taccccggaa
    17521 atgcgggaaa aaattggtga tgcagcagtg atggtggcta aatcctgcaa ctatgttagc
    17581 accggtacag tagaattcct ggtggatgat aaactgaatt tttatttttt ggaaatgaat
    17641 acccgcctgc aggtggaaca cccggtttct gaaatgatca tcggtcttga tctggtagaa
    17701 caacagatca ggatagcaag aggtgaaaaa ttaggtatta cccaggatga catcaaaccc
    17761 aacgggcatg ctattgaact cagggtctat gcggaagatc cgcttaataa ctttctgcct
    17821 tccatcggca ccttaacgaa atatgaaaaa ccaaccggtg aagggatacg ggtagatgat
    17881 gggtatgagg aaggaatgac gataccgatt tattatgatc ccatgatcgc caaactggta
    17941 gtgcatggca aaacaagaac agaagcgatt cagaaaatgt tacaggctat taaggagtat
    18001 aagatcgaag gtgtcgctac caccttacct tttggtacgt ttgtttttga gcatgatgca
    18061 ttcttctccg ggaaatttga cactcacttt gtaaagaatt actatacacc tgaaaaaata
    18121 aaagcaaaac aaaaagcgaa tgcagagatt gctgccattg ttgcgataaa atactggcag
    18181 gaaagaaaga gaatgcttat tcctgtagaa cataaatcca ccagttggaa acgtcgtttg
    18241 gcacattgat tcattaattg aacagaaacc ttatttcatt caaaaaaata atacatgaaa
    18301 ccgaaagttg aaatattaag agagaagata gaagaaggaa aattgggagg aggagtaaaa
    18361 agaatagaag cgcagcataa gaaaggaaaa ctgactgcca gggaaagggt agagctactg
    18421 atggacccgg gatcttttga agagatcggg gcattggtca cacaccggac taaagatttc
    18481 ggcatggaaa accagaagta ttatggggat ggtgtcatca ctggttttgg aaccgtcaac
    18541 ggaaggttag tgtatgtctt tgcacaggac tttacggtat ttggaggagc cttgtctgaa
    18601 actcatgctg aaaaaatttg ccgcattatg gatatggcta tgaaatcagg tgcgccgttg
    18661 gttggattga atgactcagg cggtgcaagg atacaggaag gtgtccggtc attaggcggg
    18721 tacgcagata ttttttacag gaatgtacag gcatcaggtg tgatcccgca aatttcagcc
    18781 atcatggggc cctgtgccgg tggcgctgtt tattcacctg ctatgactga ttttactatc
    18841 atggtggaga acaccagtta tatgtttgta acgggaccca atgtagtgaa gacggtaacg
    18901 aatgaagaag taagctcaga agaactgggt ggcgcctcca ctcactctac caaatcaggt
    18961 gttacacatc tcactgcggt gaatgatatg gactgcatca cacaggtaaa aaaattgctg
    19021 agctatatgc cgcaaaactg tgaagatgtg gtgatgaaac agccgtacac attgggagat
    19081 gaaacaaggg aagtgctgga aacgatagta ccggccagtg ccagtcagcc ttatgatata
    19141 aggtcagtaa ttgagggtat ttgtgataca gattctttct tcgaagttca taaagagtat
    19201 gcggataata ttgtggtggg atttgctcgg ttggccggaa gaagtttggg tattgtagcc
    19261 aatcagccga tgagcctcgc cggggtactg gatattgcca gttccaaaaa gggtgcaaga
    19321 tttacaaggt tctgtgattg tttcaatatt cctttattgg tcttggtgga tgtgccgggc
    19381 tttttaccgg gtaccgacca ggaatggaac ggcatcatca caaacggtgc aaaattgtta
    19441 tatgcactga gtgaggctac ggtgccccgt tgtactgtta ttacccgtaa agcatacgga
    19501 ggtgcttatg acgtgatgaa ctccaaacat attggtgccg atatgaatta tgcatggccg
    19561 tcagcagaaa ttgcagtgat gggagcaaag ggagccagtg agatcatttt caaaaatgaa
    19621 attgcggccg cagaagatcc tgcaaaaaaa ttactggaga aagaagcgga gtatgcggaa
    19681 ttatttgcca atccctacac ggcagctgaa agagggttta tagatgaagt gatcgaacca
    19741 agagaaacaa gaaaaaaact gatcaaggcg tttgcgatgc tggagaataa agtagcaaag
    19801 cggccgagaa agaagcatgg aaatataccg ttataaaaaa gggctcagat caattctttg
    19861 ttaacagctc ccaggaaaca caggccaata agcagcgatg gtaaaccgta taccaacgcc
    19921 attctggtat ctccgttact aagaaaataa acggccatca gcactgcacc tgccatcagc
    19981 aaccggccgc cccacagcgg ctggaaccag gcaatgataa agccggcaat tgtaagcagg
    20041 gtgaaaggaa ggaaatgtag caattcttta cccataccat tcatgatatc aggtaaacct
    20101 tccccaataa agaaactaag aaaaaaggcc gtaaccagta tgccaccgat acggcctgtc
    20161 cataaaagta atttgtgctg ttgcatacaa tttctttaaa cagtttgact cactgcatct
    20221 attcacattg ctttcagtac aggttgctct ttcaggttaa ccagtacatg ttgctttact
    20281 aaatccgttg cgatcatttg cccgtactca tgcccgaggc tttcggaaga tgaagggtcg
    20341 aaggattggc tctgtgcaga gtagaccagg tcattcgttg ccaggtcgta caggttagtt
    20401 tcccagaagt acttggtatc ttctgtata
//
LOCUS       DAORTI010000057        20332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2243_length_20332_cov_6.727376, whole genome shotgun sequence.
ACCESSION   DAORTI010000057 DAORTI010000000
VERSION     DAORTI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_20332_cov_6.727376"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /gene="purL"
                     /locus_tag="PLO70_08910"
     CDS             complement(<1..1716)
                     /gene="purL"
                     /locus_tag="PLO70_08910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQZ74626.1"
                     /translation="MEVTVKTAQQLRLTADEFELIRQKMGRTPNFNELCAFSGMWSEH
                     CSYKNSIKWLKTLPREGGRMLVKAGEENAGLMDIGDGLGVVFKIESHNHPSAIEPFQG
                     AATGVGGIHRDIFTMGARPIASLNSLRFGNLEEDKTQHLLSGVVHGIGHYGNCFGVPT
                     VGGEIYFDECYHTNPLVNAMSVGIVKAGETVSATALGKGNPVMFVGSATGKDGIGGAS
                     FASADITAESAQDLPAVQVGDPFQEKKLLEACLEVIQTGAVVGMQDMGAAGIICSTAE
                     MSAKGEVGMRIDLDKVPTRQKNMKTWELLLSESQERMLLVAEKGREDEVQKVFEKWDL
                     PCSVIGEVTDDGILNFYMNGELEASLPAYELVLGGGAPQYEREYTTPKYFADIEKFNP
                     EAVVVPDDLRSVAEQIVQLPSIASKRWIYNQYDSMVGTNNTSTNAPTDAPLVIVKGTT
                     KALAVTTDCNSRYVFADPCKGTMIAVSEAARNIVCSGGQPLGVTNCLNFGNPYDPEVY
                     YQFVHAIKGMGEACRKFDTPVTGGNVSFYNESPDYAVYPTPTIGMVGLIEDIEFMVTS
                     YFKNEG"
     gene            1815..3119
                     /locus_tag="PLO70_08915"
     CDS             1815..3119
                     /locus_tag="PLO70_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74627.1"
                     /translation="MRDNFITLEYLLFIVYLVLFAWLVTRVKFFALSGLTKPQLIIIF
                     LLKVMAGIFYGWIGVYYGGLAQMWDTWRFHVESLQEYQLLQTNPQEFFTNLFRDPYQG
                     GFSKFFETTDSYWNDLKGNIFIKILSIFNILSLGHYYVNVIFYSFITLFGSIAIFRVM
                     NDVFPGKKLTILLATFLVPSFLYWTSGIHKEGLIFTGIALVIYSIYFGNKEKKYSFQK
                     IAIAITGLFLILTLRNFLFVIIIPAILTWIIANRWPQKGLIIFSSLYVIFSIVFFTAR
                     YISPSLDFPQAVVNKQQEFIKLIGTTSVPIKELEPSVRSFSKNTPQAITLSTIRPYPS
                     DVHHILSLAAAIEINLLLLFFILFLLFRTNGIKSKNLIYFCLFFAFSVLLSIGFSVNN
                     LGAIVRYRSIIIPLLVIPMIAQIDWSRLNSYFFKNIKKNNYI"
     gene            3255..5444
                     /locus_tag="PLO70_08920"
     CDS             3255..5444
                     /locus_tag="PLO70_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQZ74628.1"
                     /translation="MSLRFNAIHNLTTKTDVKVDSAAKITGIFGSNVFTLKTAREFLS
                     DEAYKSLQSSIKAGHKIDRSMANQIANGIRAWAEAKGVTHFTHWFQPLTGTTAEKHDS
                     FFTLKGDGTPIEQFEGDALVQQEPDASSFPSGGLRATFEARGYTAWDPSSPPFIMEIA
                     QGKTMCIPTIFVSYTGESLDYKAPLLKALDALNKAAVDVCNYFDKNVTRVTATLGWEQ
                     EYFVIDEALFNARPDLLMTGRTVYGHTSAKNQQLEDHYFGSIPERIYTYMRDFETEAY
                     KLGIPLRTRHNEVAPAQFECAPIFEEVSVAVDHNSLLMDVMDRVARRHNLRVLLHEKP
                     FAGINGSGKHNNWSMATDTGVNLLAPGKTPKTNLMFLTFFVNTIKAVHDYADILRASI
                     ASAPNDHRLGANEAPPAIISVFIGQYLSKVLNDVKERVSDKMDETDESLLKIDIHKSI
                     PELLMDNTDRNRTSPFAFTGNKFEFRAVGSTANCANPMTVLNTIMAATLKQFKKEVDA
                     LMEKGEKKEIAIMHIIRQYIVDSEKVLFEGDGYSEAWAKEAEKRGLPNVKTTPLALDA
                     MVTDKAKKLFEDNDIYTHHELEARHEIELEKYIKKVQIESRIMGELATSHVLPASIRY
                     QNLLANNIRGLKESGLPVEAFANQTQILEKISEHINKASDLVEKMIEARKICNNMDDT
                     RTKAIAYQSQVKDTFFDDIRYHVDKLELLVDDKEWYLPKYREMLFLR"
     gene            complement(5499..6704)
                     /locus_tag="PLO70_08925"
     CDS             complement(5499..6704)
                     /locus_tag="PLO70_08925"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQZ74629.1"
                     /translation="MKMNMTFSVRIITSVISCFFAVIVQSQTGRKLHDRAILVDSHND
                     CLTSLTLKGKDIATRLETGHSDLYRWKKGGLDVQFFSIWTDKTARNKEGFYRDAVEEI
                     DSLHHIILRNPDRMTFATNYREVKKRAHKDKLVSLIGVEGGHMIENDIGKLESLYEKG
                     MRYLTLTWNNSTSWASSAMDETLFPAKLSQKGLTDFGKQIVRRMNELGIIVDLSHVGE
                     QTFYDAIAETKKPVLLSHSSVWNICPVFRNVKDDQIRAVAKNGGVICINFYSGFISKK
                     FDERQSFLNREIKDSMQRVFFADSGSDSSVMKTKWKTYYDSAMNEVRPDLAQLVDHID
                     YIVKLVGDSYVGIGSDYDGVSSVPVGMEDVTTYPKITDELQRRGYSNRSIKKILGGNV
                     LRVMKANFR"
     gene            6941..7858
                     /locus_tag="PLO70_08930"
     CDS             6941..7858
                     /locus_tag="PLO70_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HQZ74630.1"
                     /translation="MLKNSFLIVSVFCFITVIAQPPQSPLYGVNNIPLPIELDKQVCI
                     SGMKYLDTKLYFASERCPVVFVFDPETKSISNSINIQVPQNFEMEGITSCNGKLYLVS
                     ENVAAVYEVNPQNGKLRQVEISLPLPPKSKSGDGMEGIAANEKNNKFYLLRERNEDMS
                     VSQIYTYTITATNDSAEIMLKFESVIDLPLENPQWRYSDICVDSENGRLICLKSFSKG
                     KLRKQFIESIDIDEEGKLSATTLKNIPVDNFSEISNQFKDQDYSMNLEGITVDSNGTI
                     YIISDNTSGKALCDQPAKEKTILLELKKK"
     gene            8092..8499
                     /locus_tag="PLO70_08935"
     CDS             8092..8499
                     /locus_tag="PLO70_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74631.1"
                     /translation="MKRTLLILTLIFSVLGLKAQKIDSIYFHLYTDSLKKGQHNYINV
                     DGKMSNGQWQPLTAKEIQFTCPSATFKGNELIIPPDFQEKKVVVKAVLKANPSIVIER
                     TIWIKTKPDGDLPTIDQIMKGTPQEKTKNKKRQ"
     gene            complement(8548..9201)
                     /gene="fsa"
                     /locus_tag="PLO70_08940"
     CDS             complement(8548..9201)
                     /gene="fsa"
                     /locus_tag="PLO70_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQZ74632.1"
                     /translation="MKFFIDTANLAQIKEANELGILDGVTTNPSLMAKEGIKGEAAIM
                     NHYKTICEMVDGDISAEVISTEFNGIVEEGKKLAAIHPNIVVKVPMIKDGIKAIKWFT
                     DNGIRTNCTLVFSAGQAILAAKAGATYLSPFIGRIDDSNWDGIDLISQISHIYSIQGF
                     KTEILAASIRNALHIVRCAEAGADVCTCPLDSILGLLKHPLTDIGLAKFLEDAKKTA"
     gene            complement(9230..9328)
                     /gene="ffs"
                     /locus_tag="PLO70_08945"
     ncRNA           complement(9230..9328)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLO70_08945"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9381..10748)
                     /locus_tag="PLO70_08950"
     CDS             complement(9381..10748)
                     /locus_tag="PLO70_08950"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HQZ74633.1"
                     /translation="MSFSKIRWLQRFILFFALFSDIIFNDKSPMKKYTLFILLIFAFG
                     VHAQIPDHVYKSNIRTIKLFKYGDMYGYPVLTLNSNDQLELHFDDMDGDVKYYYYSFQ
                     LCNADWTPANVQPFDYIRGFQSNRIGNYRTSSIVQTRYTHYQAVFPERNSSPTKGGNY
                     LLKVFLNDDTSKLVFTKRFLAVNKKASVSGQVLQPFNGNFFQTHQRVQASVSTAAGQI
                     NPFSPQDLKVVVLQNNIWSSASLIDRPTVFRGNYYEYSDDENTTFAAGKEWRWIDLRS
                     LRLRSDRVLKIVDSDSSDRTDIYVNPDGERRQQLYVYYRDLNGIYTIENRDNANPFWQ
                     SEYAWVHFTFAPAGNRPYEGKTVYLFGELTNYSQGDDSRMVFNEEKGVYEKALYLKQG
                     FYNYSYVTLTDKKQVDFASSLENTEGNYWGTENSYTILVYFRPFGARTDELIGFANIN
                     SAFQR"
     gene            10759..11994
                     /locus_tag="PLO70_08955"
     CDS             10759..11994
                     /locus_tag="PLO70_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ74634.1"
                     /translation="MKVAGFIANRIAFNQQKTFSRFIIRLSVVATIISVAVMIVTLSL
                     ANGFQEAVSQKVFSFWGHIRIQEKQPGKAIISEETPIYKNDSLITSILQNPEVKSIHP
                     FATRYAILKTPEDIEGILVKGLDSTYDFKNLQQFIRQGRPIRFTDSTFSREILVSEYT
                     ASRMNLNVNDSIIIYFIMPGENPRPRKITITGIYKTGIEEYDRLFAIGDIKLIQQLNN
                     WQADEVGGYEIFLHDYKKIDSTANQLYNMDAFPGTWDAISVKKVSPNIFDWLNMQDVT
                     RNVLIGFMVIVAVINLITCLLILVLERVRMIGILKSLGATDWTVQKIFLRHSLIITIT
                     GIIIGAVVAIGLLWIQQETGFIKLKEEAYYLSAAAVKIIWWQVAAICAGTLIVCFLIM
                     MIPSLLVKKIQPVKAIQFR"
     gene            complement(11987..12721)
                     /locus_tag="PLO70_08960"
     CDS             complement(11987..12721)
                     /locus_tag="PLO70_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQZ74635.1"
                     /translation="MLVKSKIKYIQSLGQKKNRDEACVFIAEGPKIVSELLEADKTER
                     IEIYAVKDWIIENEKLLAKSTVIEITEEDLEKISQLKTPNKVLAIINQFATAGTIEAK
                     GKLTLALCGIQDPGNLGTIIRIADWFGIEQVVCSKDSADVYNPKVIQSTMGSIARVNV
                     FYTELEEWLAGQKGINIYATVLDGQDVTQIKKIEQGVILIGNESKGISPGILKLANAR
                     ITIPQKGKAESLNAGVATGIVLSHLT"
     gene            12774..15077
                     /locus_tag="PLO70_08965"
     CDS             12774..15077
                     /locus_tag="PLO70_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQZ74636.1"
                     /translation="MRFFLVALPAILVASCGVIPKNYPAGKPFVFQTNINVNGNFSSN
                     ERETLESRLKAQLDDSMRARSVSKLFYSVMKRPPVYDNANADRSVLFMRALLVSLGYF
                     KDSITYDTTVNIVKGDQYRTTVDFNVTPGKVVRMDSISFNIKQQELQALTIAGKNESL
                     LKKGEPFAKTTISMELDRLVDLYRNNGYLRFTRDELIGLWDTLDIELLRPSLDPFEQL
                     AVLQKLKERRENPTANLEIRLKPRFDSSKLVKYFIGNITVYPEFSADTTGIQHKEEIV
                     NGIKVISYRSTFKSKILPPNIYFRRGDLYNQRMYYKTINRFNSLGTWRLVNIEPIARK
                     GQDTADFVIRLTPAQKYSFSANLEGSNNQSVVSGNLFGIAVNTSLQNRNFAKRAIQSN
                     TNIRFGIELSDSSFVQTRQMVISHNIYFPRPIPNTNWIPEKLRDNFRSVLSFNAANTE
                     RKDLFNLTTINGSWGYDFQTKNKFFSIRYPNIEYSYLKRRKKLQDLIDNNPLLKNIFT
                     YGFIESVTGGMIWKKENKKNINSFRINGELSGLITRLVKSDFLDSNLFRFLKVDMEFA
                     RKITFKKSALVLRVFGGIGYAFNSTIDTNKRYSLPLFRQYFAGGPNSMRAWALRKLGP
                     GSFIKDFSNTSTGLPERYGDVQLEANIEYRFPWFRIAGVAVNGAVFTDIGNIWFLKKA
                     EAQGRKPEEIFNFGRLGKDLAVGVGTGLRIDFSFFIVRLDYSYKAKDPSPSPANAAGQ
                     NKWFYGYQFSDLLKGQLQLGINYPFIL"
     gene            complement(15081..15773)
                     /gene="truB"
                     /locus_tag="PLO70_08970"
     CDS             complement(15081..15773)
                     /gene="truB"
                     /locus_tag="PLO70_08970"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQZ74637.1"
                     /translation="MDKPTKLFEEGQVLLINKPLHWTSFDAVRKIRNLIRIKKVGHAG
                     TLDPLATGLLIVCTGKFTKRINEFMAQEKEYTGTITLGATTPTYDLESVPENLKSFDT
                     LTEAAIKEATRNFTGEIMQVPPAHSAIKVDGKRVYELARQGIEVKLEPRKLFIKDFEI
                     VHIELPVVHFRVVCSTGTYIRSLANDFGAALGCGGYLSSLCRTRIGEFDITNAMSMME
                     FEEKVKQLGKPE"
     gene            complement(15815..17140)
                     /locus_tag="PLO70_08975"
     CDS             complement(15815..17140)
                     /locus_tag="PLO70_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQZ74638.1"
                     /translation="MIKSFYLNNIVYYIAGSAAVVFALSYFFPALFQVGVLILVLLAI
                     AIFIDALLVYSKRNGIRAERLVSERFSIGDSNKVVINLVNQYAFNVRASIIDELPVQF
                     QERNWLKRVQLGSGAEFDLEYALRPTSRGEYIFHAINVFVHAPLKLVKRRYIFPKEQV
                     VKVYPSYMQMRRYQLLAVSNRLQEAGVKKIRKIGHSMEFEQIKEYVRGDDYRTINWKA
                     TARKQDLMVNNYTDERSQQIYCLINKGRVMKMPFHGLTLLDYAINASLVLSNVALVKQ
                     DKAGLITFEKNLDTFLPADKKPTQMNLVLETLYKQRTDFLEADFEKLFSVIRNRITNR
                     SLLVLFTNFESMESLQREMPALKKIARYHLLLVVFFENSELKSLIERKTSSIEDIYIK
                     TIAEKFTYEKKLMVKELHKNGIPSILTTPEGLTINTVNKYLELKTRMSI"
     gene            complement(17171..18148)
                     /locus_tag="PLO70_08980"
     CDS             complement(17171..18148)
                     /locus_tag="PLO70_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQZ74639.1"
                     /translation="MDEQIFHQRTDLSELNDAVLSIRNEIKKIIVGQDEMVKLIIAAL
                     LADGHVLIEGVPGVAKTLTAKLVAKSVNVGFSRIQFTPDLMPSDVLGTPVFNPKEASF
                     EFKKGPVFSNIVLVDEINRAPAKTQSALLEIMEERQATVDGKTYLMAAPFMVLATQNP
                     VEQEGTYRLPEAQLDRFLFKIVVPYPTESEEMSILTQFHSMGNTSAMDMIQPALDAAR
                     VTALRQQIKTQIVEEKLLQFIAKLIHQTRNHKSIYLGASPRASLAIMNASKAMAAMNG
                     RDFVTPDDILSVVTPVLRHRIILAPDKEMEGISEDDVIKQIIQGMDVPR"
     gene            complement(18151..19377)
                     /locus_tag="PLO70_08985"
     CDS             complement(18151..19377)
                     /locus_tag="PLO70_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74640.1"
                     /translation="MKRYTPYLLALLLATAIILLFITGDNYKNRRLDERITLRKQDKI
                     PYATYVAYNNLESLFPKATIRTSRLEPGYWDSVSMYDSRQAYLVITDRFSADDDEMRK
                     LIAFARVGNDIFISARYISSAADEAFGCGSSAYDLAFFSVSDLDEGARFSLNDPPFGD
                     TLRYRYPGKTFGSYFSTIDSSTTEILGYDESGRPNFIHLRTGKGNFYVHLEPLAFCNY
                     FLLHKDNISYYEKALSVINPGVKKIVWDEYYLYKKGEQVNKPKNEGWLSVLFRYPAFK
                     AALLTAIFTLLFYVLMEMRRKQRYIPVLKKPRNDSLDFVKTIGRLYYDKGDHKNLCHK
                     MSAYFLEHVRSKYKLPTSRLDDDFVRNLHYKSGVDEKEIGEIISFIKYLDQAAVVSQE
                     QLAHFHKQMELFYQKA"
     gene            complement(19383..20117)
                     /locus_tag="PLO70_08990"
     CDS             complement(19383..20117)
                     /locus_tag="PLO70_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74641.1"
                     /translation="MQAQDYSDTTAIIEEVIQEPPVEEEYDEEDGEADKPDFLELANE
                     DTFSLQVRKLADSLVRKMKADDDFWYADAEFEKKKEKKTSTVSPYIPIGQQTWFKTIV
                     WLVIIGVFAAGIMYYLADSNISLFRRNKKVAVVAVDEEMPGDIFTINYEKEIDKAASM
                     GNFRMAVRLMFLRLLKNMAERNIIRYQQDKTNLDYLMQLHSTAYYKDFFRITRNYEYS
                     WYGKFEVSQDAYQFIRNDFEQFENRI"
BASE COUNT         6122 a         4317 c         3718 g         6175 t
ORIGIN      
        1 cccttcattc ttaaaatatg aagtaaccat gaattcaata tcttctatta atcctaccat
       61 accgattgta ggggtggggt aaactgcgta atccggtgat tcattgtaaa aacttacatt
      121 accgccggta accggtgtat caaacttacg gcatgcctct cccattcctt taatagcatg
      181 gacaaactgg taatacactt ccgggtcgta aggattacca aaattcaaac agttggtaac
      241 acctaatggc tggccaccac tgcaaacaat attcctcgct gcttcactta ccgctatcat
      301 ggttccttta cagggatcag caaaaacata acggctattg caatcagtag ttactgccag
      361 ggctttggtt gtgcctttca caataaccaa tggtgcatca gtcggcgcat tggtagatgt
      421 attattagta cccaccatac tgtcgtattg attatatatc catcttttag atgcaatgga
      481 aggaagctgt actatttgct cagcaacaga tctcaggtca tcaggcacaa ccactgcttc
      541 aggattaaat ttttcgatat ctgcaaaata ctttggtgtt gtatattctc tctcatactg
      601 cggcgcacct ccacctaata caagttcgta tgcaggcaat gaagcctcca gttcaccatt
      661 catatagaaa ttcaatatac cgtcatctgt cacctcacca atcacagagc agggcaaatc
      721 ccatttttca aatacctttt gaacttcatc ttcccttcct ttttcagcaa ccagcaacat
      781 tctttcctgg ctttcactca gcaacaattc ccatgtcttc atattttttt gacgtgtagg
      841 cactttatcc aggtcaatcc gcatacctac ttcgccttta gcactcattt cagctgtcga
      901 gcaaattata cctgcggctc ccatatcctg cattcctact acagccccgg tttgtatcac
      961 ttccaggcag gcttccaata gttttttctc ctggaatggg tctcccacct gcacagctgg
     1021 taaatcctgt gcactctcag cagtaatatc tgccgacgca aaagaggctc cgccaattcc
     1081 atctttaccg gtagcactac ctacaaacat tacagggtta ccttttccca atgcagttgc
     1141 agaaacagtt tctcctgctt tgacaatacc cacactcatt gcattcacca atggattggt
     1201 atgatagcat tcatcaaaat atatttcacc accaacggtc ggtacaccaa aacagtttcc
     1261 gtaatgacca atgccatgta ctaccccgga aagtaagtgt tgtgttttat cttcttctaa
     1321 atttccaaat cgaagtgagt tgagtgaagc aattggcctt gcccccatgg taaaaatatc
     1381 acgatgaata ccacccactc ctgttgcagc cccctggaat ggttcaatag cagatggatg
     1441 attatgtgat tcaattttga aaacaacacc gagtccgtct ccaatatcca ttaatcctgc
     1501 attttcttca cctgctttca ccagcattct tccaccttct cttggtaagg tctttagcca
     1561 tttgatagaa tttttataac tgcagtgttc actccacatt ccggaaaaag cacagagttc
     1621 attaaaattg ggagttcgtc ccattttttg cctgatcaat tcaaattcat cggcggttaa
     1681 tcgtagctgt tgagcagttt ttactgttac ttccatgtaa gctaaatatt gagttgcaaa
     1741 tttaaagatt gtcggtcaca gcttgaacag aacttgtcaa ctttgtgctg acaataacgg
     1801 taataatcta tattttgcgg gataatttta ttacactgga ataccttctt tttatagtct
     1861 atcttgtatt gtttgcctgg ctggtaacca gagttaaatt ctttgccctt tcggggttaa
     1921 caaagccaca gcttattatc attttcctgt taaaagtaat ggcaggaatt ttttatgggt
     1981 ggatcggagt gtattatgga ggattggcgc agatgtggga cacctggaga ttccatgtag
     2041 aaagcctgca ggaataccaa ctccttcaaa caaatccaca ggaattcttt accaacctgt
     2101 tcagagatcc gtatcaagga ggattttcaa aattctttga aaccaccgac tcttactgga
     2161 atgatctgaa aggtaatatc ttcattaaaa ttctttccat tttcaatatc cttagtcttg
     2221 gtcattatta tgtaaatgtt attttctact cttttattac cctttttgga tcaattgcca
     2281 ttttcagggt aatgaatgat gttttccccg ggaaaaagct gacgatcctg ctggctacct
     2341 ttcttgttcc ttcatttctt tactggacaa gtggtattca taaagaggga ctcattttta
     2401 caggaattgc tttagtcata tacagtatat atttcggcaa taaagaaaaa aaatacagtt
     2461 ttcaaaagat tgccattgca attaccggcc tgtttctaat acttaccctg cgcaattttc
     2521 ttttcgtaat cataatacct gcaattttaa cctggattat tgctaaccgc tggccgcaaa
     2581 aagggcttat tattttcagt tctttgtacg ttattttttc aattgttttt tttacagccc
     2641 gctacatcag cccttcactt gattttcccc aggcggttgt aaataaacaa caagagttta
     2701 ttaagttgat aggaactaca tccgtcccca ttaaagaact ggaacccagt gtaaggagtt
     2761 tttcaaagaa caccccccag gcaataactc tttcgactat aaggccctac ccaagtgatg
     2821 tccatcatat actttcactt gctgccgcta ttgagataaa cttgctattg cttttcttta
     2881 tcctattcct cctattccgt acaaacggaa taaagtcaaa aaaccttatt tacttctgct
     2941 tattttttgc tttttccgtg ttgctgtcca taggtttcag tgtaaataac ctgggagcaa
     3001 ttgttcgcta tcgcagcatc attataccgc tgctcgtcat tccaatgatc gcacagattg
     3061 attggtcaag gttaaattct tattttttta aaaacattaa gaaaaataat tatatttaaa
     3121 atataaagta aaatccatct tttgttttaa aataaaattg attttatcac atttttcccc
     3181 caatgactgt tgttttcaaa taaataattg gaataattac aacaaatcct ttgttttgct
     3241 gaaaactatt tcacatgtca cttcgtttta atgcaattca caatttaact accaaaaccg
     3301 atgtaaaggt tgatagtgct gccaagatta ccgggatatt tggaagcaat gtttttacac
     3361 taaagactgc ccgtgagttt ttaagcgatg aagcttacaa aagtcttcaa tcttccatta
     3421 aagccggtca caaaattgac aggtcaatgg ctaaccagat agctaatggt atccgtgcct
     3481 gggctgaagc gaaaggggta acccatttca cccattggtt tcagccgctt acaggtacaa
     3541 cagcagaaaa acatgattct ttttttacct tgaaaggaga cggaactcca atagagcaat
     3601 ttgaaggtga tgcattggta caacaagagc ctgatgcttc ttcgtttccc agcggtggat
     3661 taagagccac ttttgaagcc cggggatata ctgcatggga tccatcttct cctcccttca
     3721 taatggaaat tgcccagggt aaaacaatgt gcattcccac aatctttgtt tcatacacag
     3781 gcgaatcctt agattataaa gcacctttgc tgaaagcact ggatgcattg aacaaagcag
     3841 ctgttgatgt atgtaattat tttgacaaaa acgtaacaag agtcacagct acccttggat
     3901 gggagcagga atattttgtg atagatgaag cattatttaa tgcaagaccc gacctgttaa
     3961 tgaccggaag aactgtttat ggtcacactt cagcaaaaaa ccagcaattg gaagaccatt
     4021 acttcggctc tatccctgag agaatctata cctacatgag ggattttgaa acggaagctt
     4081 ataaactagg catcccttta cgtaccaggc ataatgaggt agcccccgca caatttgaat
     4141 gtgcccctat atttgaagaa gtgagtgtgg ccgttgacca taattctctg ttgatggatg
     4201 taatggacag ggtggcccgt cgtcataacc ttcgggtatt gcttcatgag aaaccttttg
     4261 caggtatcaa tggaagtggc aaacataaca actggagtat ggcaactgac accggtgtaa
     4321 accttttagc acctggcaaa acgccaaaga ccaacctgat gttccttaca ttctttgtga
     4381 ataccataaa ggctgttcac gattatgctg atatactaag agcatccata gcctctgcac
     4441 caaacgatca ccgtcttggc gccaatgaag cacctcctgc aattatatcc gtatttatcg
     4501 ggcaatatct ttctaaagta ttgaatgatg taaaagaaag agtgtctgac aaaatggacg
     4561 aaactgatga aagtttactt aaaattgata tacataaaag tatcccggaa ttgttaatgg
     4621 ataatacgga ccgtaaccgt acttctcctt tcgctttcac tggtaataaa tttgagttcc
     4681 gtgctgtagg ttctactgcc aactgtgcta acccaatgac tgttttaaat actataatgg
     4741 ctgccaccct gaaacagttt aaaaaagaag tagatgcttt aatggaaaaa ggggaaaaga
     4801 aggagatagc cattatgcat attatccgcc agtacattgt tgatagtgaa aaggttttgt
     4861 ttgaaggcga tgggtatagt gaagcatggg caaaagaagc tgaaaaaaga ggattaccaa
     4921 acgtaaagac aactcctctt gcattagatg ccatggtaac agacaaggcc aagaaactat
     4981 ttgaagacaa tgacatctat acccaccacg agctggaagc caggcatgag attgaacttg
     5041 aaaaatacat caagaaagtt cagattgaaa gccggatcat gggtgaattg gctaccagcc
     5101 atgtcttacc tgcttctata cgttatcaga acttactggc aaacaatatc cggggattaa
     5161 aagaatccgg acttcctgtt gaagcttttg caaatcagac acaaatactg gaaaaaatct
     5221 ctgaacatat aaataaagcc agcgacctcg ttgaaaaaat gatcgaagca aggaaaattt
     5281 gtaataacat ggacgatacc cggacgaaag ctatagcgta tcagagccag gtaaaagata
     5341 ctttctttga tgatatccgg taccatgtag acaaactgga attgctcgta gacgacaaag
     5401 aatggtattt accaaaatac agagaaatgt tattcttaag ataaatgcat tcgttttctt
     5461 cctgataata aaaccccgga acttaccggg gttttatttt atctgaaatt tgctttcatc
     5521 acccgcagta cattccctcc cagtattttt ttgatggatc tgttagaata tccccttctt
     5581 tgcaattcat cagtaatctt cggataagta gttacatctt ccattcctac cggaaccgag
     5641 cttactccat catagtctga accaattcca acataactat caccaactaa ttttacaatg
     5701 taatcaatat gatcaactag ttgtgccagg tccggcctta cctcattcat tgcagaatca
     5761 taataggtct tccattttgt tttcataaca gaagaatcgc tcccactgtc tgcaaagaaa
     5821 acccgttgca tagaatcctt tatttctcta ttgaggaatg attgacgctc atcaaacttt
     5881 ttactgatga agccggaata aaaatttata cagatcacac ccccgttttt tgccacagcc
     5941 cttatctgat catccttcac atttctgaat acagggcaaa tattccagac agaactatgt
     6001 gagagcagaa cgggtttctt cgtttctgcg atagcgtcat aaaaagtttg ttccccgaca
     6061 tgagaaaggt ctactataat tcccagttca ttcatcctcc tgactatttg tttaccaaaa
     6121 tcggtaagcc ctttttgcga aagcttggca ggaaataatg tttcatccat cgcactggag
     6181 gcccaactcg tactattatt ccaggtaagt gtcaggtatc tcattccctt ttcatacagg
     6241 ctttcgagct tgcctatatc attttctatc atatgccccc cttctacacc gataagtgaa
     6301 accagtttat ccttatgtgc cctttttttt acctccctgt agttggtagc aaaagtcatc
     6361 ctgtcgggat tgcgaaggat gatatgatgc agagaatcta tttcctcaac agcgtctctg
     6421 taaaaacctt ctttatttct tgctgtttta tctgtccaga tggaaaaaaa ctgaacatcc
     6481 aaacctcctt tcttccacct gtacaaatcg ctgtgccctg tctcaagacg agtggcaata
     6541 tccttacctt ttagtgttaa tgatgtcagg caatcattat gcgaatcaac cagaattgcc
     6601 ctatcatgca attttcttcc cgtttgtgac tgaactatca ctgcgaaaaa acaagaaatt
     6661 acagaggtta ttatccgtac actgaaagtc atattcattt tcattataaa aattcgattt
     6721 agcttacaat caaccagcaa tatcaataaa aggtagattt ccctgctttg tgtgtatcat
     6781 tttgccagcg cttattgctt taacctgctg cttaatacta aatttagtaa aacttattat
     6841 cttacacact aatcactatt acaacctttg taaaaagtcc ggcgtctgtt tactcccgga
     6901 tagtacaaac cgggggtgta gctaataaaa ataactttgc atgctgaaaa atagtttcct
     6961 gattgtaagt gtattttgtt ttataactgt aatcgcacaa cccccgcaat caccccttta
     7021 tggcgtaaat aatattcctt taccaattga actggataaa caagtctgca tttcaggaat
     7081 gaaatacctg gatactaaat tatacttcgc atctgaacga tgcccggttg tttttgtctt
     7141 tgatccggaa acaaaaagta tcagcaacag catcaacata caggtaccgc agaattttga
     7201 aatggaaggt atcacttcct gcaatggcaa attatacctg gtgtctgaaa atgtggctgc
     7261 tgtgtatgaa gtcaatcctc aaaacggcaa actcaggcaa gtagagatct ctttaccact
     7321 gccccccaaa tcaaaaagtg gtgatggtat ggaaggtatt gctgccaatg aaaagaataa
     7381 taaattctac ctgttacggg aaagaaatga agacatgtca gtatcacaga tatataccta
     7441 tactattact gcaactaatg acagtgctga aattatgttg aaatttgaat ctgtaattga
     7501 tttgccgctg gaaaatccgc agtggagata ctcagatatt tgtgtggaca gcgaaaacgg
     7561 aaggcttatc tgtcttaaat cgttctcaaa aggcaaactt aggaaacagt ttattgaatc
     7621 aatagatata gatgaagaag gtaaattatc tgctactaca cttaaaaata ttcctgtcga
     7681 taacttttct gaaatatcca accagtttaa ggaccaggat tatagcatga accttgaagg
     7741 cattacggtt gatagcaatg gcactatcta tatcatcagc gacaatactt ccggcaaggc
     7801 cctttgcgac caacccgcaa aagaaaaaac tattttgctg gaactcaaaa agaaataaag
     7861 tcttgattta tatttctttg agtaattcgt gcaagcctta tacctgtaag tgttgctgat
     7921 tttgtataat ctaaagtata ttcactcttt aattttccgg atgaagtaaa tgattcctga
     7981 gaaggcttca gtcccttaca atgaatagta tgaagtggct tttctataaa ttctgttaac
     8041 aaatttcatt aaacaacttt ttggcctgaa ttttcgactt tactatatat catgaaaaga
     8101 acgctactca ttctaacact tattttttca gttttgggac tcaaagccca gaaaattgac
     8161 agcatttatt tccatctcta taccgacagc cttaaaaaag gacaacataa ttatatcaac
     8221 gtagacggga aaatgtcaaa cggacaatgg caaccactaa ctgctaaaga aatacagttt
     8281 acctgtccat ctgccacatt taaagggaat gaattaataa ttccgcctga tttccaggaa
     8341 aagaaagtag tagttaaggc agtgttgaaa gcaaacccct ccattgttat tgaacgaacg
     8401 atctggatca aaacaaagcc agacggggat ttacctacga ttgatcaaat catgaaaggc
     8461 actccacagg aaaaaacaaa aaataaaaaa cgacaataaa aaagcccctg gaagtccggg
     8521 ggcttttaac aagttgctgt ttatttttta tgcggttttc ttagcatctt ccaaaaactt
     8581 agccaggcct atatctgtca atggatgttt caataaaccg aggatagaat caagggggca
     8641 ggtgcaaaca tctgcaccgg cttcagcaca tcttacaata tgtaaagcgt tgcggatgga
     8701 agcagccagt atttcggttt tgaaaccctg tatagagtat atatgcgaaa tctgtgaaat
     8761 aagatcaata ccatcccagt tgctgtcatc tatacggcca atgaaaggag acaggtaagt
     8821 agcgcctgct tttgcagcca ggattgcctg tccggcagag aaaaccagcg tacaatttgt
     8881 cctgataccg ttatcagtaa accatttgat tgcttttatc ccatctttga tcatgggtac
     8941 ttttactaca atattcgggt gaatagcagc cagtttcttt ccctcttcaa caataccatt
     9001 aaattcagta cttattactt cggcactgat atcaccatcc accatttcac aaatggtttt
     9061 ataatgattc ataatggccg cttcgccttt tatgccttct ttagccatca gggaagggtt
     9121 agttgtaaca ccgtcaagaa tgcctaactc atttgcctct ttgatctgag ccaggttcgc
     9181 tgtatcaata aaaaatttca taaaacaggg aattggtaat gaagaaaaaa aatggcaggc
     9241 tgattacatc gcaccgctca accacctact cttgctgcct tccggccctg ggagggttca
     9301 gcaggagctg gtcgtgtaag acctgccgcc gcaaagataa gggagcaaca tttaataggg
     9361 aagatgaatt aaaaaaactc tcagcgctga aatgcagaat ttatgtttgc aaagccaatt
     9421 aactcatctg ttcgggcacc aaatggacgg aaataaacca aaatagtata actattttca
     9481 gttccccagt aatttccttc tgtattttcc agggatgatg caaaatcaac ctgtttcttg
     9541 tctgtcagcg ttacgtaaga ataattataa aatccctgct tcagataaag ggctttctca
     9601 tacacccctt tttcttcatt gaaaaccatc cgtgaatcat caccctggct gtaattggta
     9661 agctccccaa aaagatagac agttttacct tcgtaagggc ggttgcctgc cggggcgaaa
     9721 gtaaagtgga cccatgcata ttcactttgc cagaaaggat tagcattatc acggttctcg
     9781 atagtataga tgccattcag atcacggtaa taaacatata gttgctggcg acgttctcca
     9841 tcaggattta cataaatatc tgtacggtcg ctggaatcgc tatcaactat tttcaatacc
     9901 ctgtcactcc ggagcctgag acttcgcagg tcaatccacc gccattcctt tcccgcagca
     9961 aatgtggtat tctcatcgtc actgtattca taataattac ccctgaacac agtaggccgg
    10021 tctatcagtg atgctgacga ccatatatta ttttgcaaca caaccacttt caggtcttgc
    10081 ggtgaaaatg gattaatctg cccggcggcc gtacttacag aagcctgtac ccgctgatgt
    10141 gtttgaaaaa aattaccatt gaaaggttgc aaaacctggc ctgatacaga tgccttctta
    10201 ttgactgcga ggaatcgctt tgtaaaaacc agctttgatg tatcgtcatt caggaaaacc
    10261 tttaacaaat agttcccgcc tttagtagga gatgaatttc tctcgggaaa aacagcctgg
    10321 taatgagtgt aacgggtctg cacaattgat gaggtgcgat aattaccgat gcgattactt
    10381 tgaaaacccc ggatataatc aaatggctga acattagccg gcgtccagtc tgcattacat
    10441 aactgaaaac tgtaatagta atacttcaca tccccatcca tatcatcaaa atgcagctcc
    10501 aattggtcat tactattgag tgtaagtaca ggataaccgt acatatcacc atacttaaaa
    10561 agctttatag tacggatatt tgatttatat acatgatcag gtatctgggc atgaacacca
    10621 aaagcaaaaa ttaacagaat aaataaagta tattttttca tcgggctctt atcattgaaa
    10681 ataatatctg aaaataaggc aaaaaacagg ataaatcttt gtaaccacct tatttttgag
    10741 aaagacaaac aattcatttt gaaggttgca ggctttatag caaatcgcat agcatttaat
    10801 cagcaaaaaa ccttttccag gtttatcatc cgcttatcgg tggtagctac cattatcagt
    10861 gtggcggtaa tgattgtcac cttatcactt gccaacggtt tccaggaggc agtgagccag
    10921 aaagtattca gcttttgggg acatatccgt atccaggaaa agcaacctgg caaagccata
    10981 atttcggaag aaactcctat ttataaaaac gattcattga ttacttccat acttcaaaat
    11041 cctgaagtaa aaagtataca tccatttgct acccggtatg caattctgaa aacgccggaa
    11101 gatattgaag ggatcctggt aaaaggtctc gatagcactt acgattttaa gaacctgcag
    11161 caattcatca ggcaagggag gcctatccgt tttacagaca gcactttcag cagggaaatc
    11221 cttgtttcag aatacaccgc ctcccgcatg aatttaaatg taaatgacag tatcatcatt
    11281 tattttatca tgcccggtga aaatccccgg ccacgaaaaa taaccatcac tggtatttat
    11341 aaaacaggca ttgaagaata tgacaggctt tttgctatcg gagatatcaa actgatccag
    11401 caactaaata actggcaggc agacgaagtg ggtggttatg aaattttttt acatgattat
    11461 aaaaagatag acagcactgc caatcaacta tacaatatgg atgcatttcc aggcacctgg
    11521 gatgccatca gtgtcaaaaa agtttctccc aatattttcg actggttgaa tatgcaggac
    11581 gtaacaagaa atgtattgat tggcttcatg gttatcgttg ctgttattaa ccttatcacc
    11641 tgtttgctga tcctcgtact tgaaagagtg agaatgatag gaattttaaa atcattgggt
    11701 gccacagact ggaccgttca gaagatattt ctccgccatt cacttatcat cactattaca
    11761 ggcatcatta tcggtgcagt tgttgcaatt ggccttttat ggatccagca agagaccggc
    11821 tttatcaaat tgaaagaaga agcctattat ctcagtgcgg cggctgtgaa aattatctgg
    11881 tggcaggtag ccgccatctg tgcaggcact ttaatagtat gttttcttat tatgatgata
    11941 ccttcactat tggtaaaaaa aatccaacct gtaaaagcaa tacaattcag gtaagatgtg
    12001 acaagacgat tccggtagct actcctgcat taagcgactc cgcttttccc ttctgaggaa
    12061 tagtaattct ggcatttgca agttttaata ttcccggact gatccctttt gactcattgc
    12121 caattaatat gaccccctgc tctattttct ttatttgggt tacatcctgt ccatccaaaa
    12181 ccgtagcgta aatatttata cctttttggc cggcaagcca ctcttctaac tctgtataga
    12241 agacatttac ccttgctata cttcccatcg tagactgaat caccttggga ttataaacat
    12301 ctgcagaatc tttgctgcaa acaacctgtt ctataccaaa ccaatccgca atccggataa
    12361 tagtgcccag gtttccggga tcttgaattc cgcataaagc caatgtgagt ttaccctttg
    12421 cctcaattgt tccggcagta gcaaattgat ttataattgc aagaaccttg tttggcgttt
    12481 ttaattgtga aattttctct aaatcctcct cggtaatttc aatcacagtt gattttgcta
    12541 atagtttttc attctcaatt atccagtctt ttactgcata tatctctatt cgctcagttt
    12601 tatcagcttc cagcaattct gaaacgattt ttggcccttc cgcaataaag acacaagctt
    12661 catccctgtt ttttttctgg cctaaacttt ggatatattt gatcttcgat ttgactagca
    12721 tacactttct tttaaaatct atgttaacag gccagccatt tagctttaaa aaattgcgat
    12781 tttttctcgt ggctctgcct gctatacttg tggcctcctg tggggtaata cctaaaaact
    12841 acccggccgg taagccgttt gtatttcaga caaatatcaa tgtaaatggg aacttttcaa
    12901 gcaatgaaag ggaaacactt gaatcccgcc tgaaagccca gttggacgat agtatgcggg
    12961 cacggtctgt gtcaaagctt ttttacagtg tcatgaagag gcccccggtc tatgataatg
    13021 cgaatgccga cagatctgtg ctttttatgc gggctttgtt ggtttcctta ggatatttta
    13081 aagattcaat tacttacgac acaactgtca atattgtaaa aggtgatcaa tacagaacta
    13141 ctgtagattt taatgttacg cctggcaaag ttgtcaggat ggattccatc agttttaata
    13201 ttaaacagca ggaattacaa gctcttacta ttgccggtaa aaatgaatcc ctgctgaaga
    13261 aaggagaacc ttttgccaaa acaaccatat caatggaact tgaccggttg gttgacttat
    13321 acaggaacaa tggttatctc cgttttacac gggatgaact tatcggtctt tgggacacgt
    13381 tggatattga acttttgcgt ccttcactgg atccgtttga acaactggca gttttacaaa
    13441 aattaaaaga aagaagggaa aatccaaccg caaaccttga aataagatta aaacccagat
    13501 ttgacagcag caaactggta aaatatttta ttggtaacat aactgtttat ccagaattca
    13561 gtgctgatac aaccggaatt caacataaag aagaaatagt aaacggaata aaagttattt
    13621 cttaccggag tacttttaaa tcaaagattt taccgcccaa tatatatttc cgcagaggtg
    13681 acctttataa ccagcgaatg tattacaaga caatcaaccg ctttaattca ttaggcacct
    13741 ggcgattggt caacatagaa ccaatagcaa ggaaaggaca ggataccgca gattttgtaa
    13801 ttcgccttac cccggcgcaa aaatattcgt tcagtgcaaa cctggaaggc agtaataatc
    13861 agagtgtcgt ttcaggcaac ctgtttggga ttgctgtcaa tacaagtctt caaaaccgaa
    13921 attttgccaa acgggccata caatccaata ccaatattcg ttttggtata gaattaagtg
    13981 acagcagttt tgtacaaaca cggcaaatgg tcatcagtca taatatttat tttccccgcc
    14041 ccatccctaa tactaactgg attcctgaaa aattaagaga taatttcagg tctgtattgt
    14101 cctttaatgc agctaatacc gaaagaaagg atctttttaa tcttaccacc atcaatgggt
    14161 cctggggtta tgactttcag acaaagaata aattcttttc catacgctat ccgaatattg
    14221 aatattctta cttgaaaaga agaaaaaaat tacaagattt gatcgacaat aacccgttac
    14281 taaaaaatat ttttacatac ggatttatag aatcggtcac agggggaatg atctggaaaa
    14341 aagaaaataa aaagaacatt aattcattca ggattaatgg ggaattgtca ggattaatta
    14401 caagacttgt aaaaagcgac ttcctggatt ccaatctttt ccggttcctg aaagtggata
    14461 tggaatttgc cagaaaaatt acatttaaaa aatcggcgct tgtgctaagg gtcttcggag
    14521 gtattggcta tgcctttaat tcaactattg atacaaataa aagatacagc cttcccttat
    14581 tcagacaata ttttgccggc ggccctaata gtatgcgggc atgggcgctg cgaaaactgg
    14641 gaccgggatc ttttatcaag gatttcagca ataccagcac ggggctccct gaacgatatg
    14701 gagatgtaca actggaggca aatattgaat atcgtttccc ctggttcagg atcgccggcg
    14761 tagcagtaaa tggcgctgtt ttcaccgata tcgggaatat atggtttttg aaaaaagcgg
    14821 aggcccaggg cagaaaacca gaagagattt tcaatttcgg caggctgggc aaagacctgg
    14881 ccgttggtgt agggactggc ctaagaatag attttagttt ttttattgtc aggcttgatt
    14941 attcgtataa ggccaaagac ccaagtccat cacctgcaaa tgccgctggc cagaataaat
    15001 ggttttacgg ataccaattt tctgatctgt taaaaggaca actacaatta ggtatcaact
    15061 atcctttcat tttgtaaata ttattcaggc ttaccaagct gttttacttt ctcttcaaat
    15121 tccatcatag acattgcatt agtaatgtca aactccccta ttcttgtacg acaaagactg
    15181 ctgagatatc ctccgcaacc cagtgcagct ccaaaatcat tcgcaaggct gcgaatataa
    15241 gtgccggtag aacaaactac ccggaaatga acgaccggta attcaatatg aactatttca
    15301 aaatctttta tgaatagctt tcggggttcc agtttcactt ctattccctg gcgggcaagc
    15361 tcatacaccc ttttcccgtc aactttaata gcagaatgag ccggtggcac ctgcattatt
    15421 tcaccagtaa aattccgggt ggcttctttt atggctgcct cggtaagtgt atcgaatgat
    15481 ttcaggtttt caggcacact ttccaggtca taggtgggag tggtagcacc aagtgtaatt
    15541 gtgcccgtat attctttttc ctgggccata aattcattga tacgtttggt aaattttccg
    15601 gtgcaaacga tcagtaatcc tgttgccagg ggatcaagtg ttcccgcatg accaactttc
    15661 ttaatacgaa tgagattcct gatttttctt actgcatcaa aagatgtcca atgcaagggc
    15721 ttattgataa gtaatacctg accttcttca aataatttag ttggcttatc cattacagca
    15781 aaagtaagga aggtgatgca gaccaaagga caacctaaat agacatcctt gtttttaatt
    15841 cgaggtattt gttaaccgta ttaattgtaa gtccctcagg tgtagttaaa atggaaggta
    15901 tgccattctt atgcaattct tttaccatga gttttttttc ataagtgaat ttttctgcaa
    15961 tagtttttat ataaatatct tctatagaag aagtttttct ttctatcagg gattttaact
    16021 ctgagttctc aaaaaagact actaataaca aatgatacct ggcaattttt ttcaatgccg
    16081 gcatttcccg ctgcaggctt tccattgatt caaaattggt gaataaaacc agcaggcttc
    16141 tgttggttat cctgttccgg ataacagaga ataatttttc aaaatccgct tccaggaaat
    16201 cagttctttg cttatacagc gtctccagta ccaggttcat ctgggtgggc tttttatccg
    16261 ctggcaaaaa agtgtcaagg ttcttttcaa aagtgatcaa gccggcttta tcttgcttaa
    16321 ccaacgccac attggataag accaatgacg cattgatagc ataatccagc agggttaatc
    16381 catgaaaagg cattttcatc acccttcctt tattgatcag gcaatatatc tgctgacttc
    16441 tttcatcggt atagttattc accatcaggt cttgtttcct ggccgttgct ttccagttga
    16501 tggttcggta atcatctccc ctgacatatt cttttatctg ctcaaactcc atactgtgtc
    16561 ctatcttcct gatcttttta acacctgcct cctgcaggcg attactgaca gccaataact
    16621 ggtagcggcg catctgcata taagatggat acactttaac tacctgttct ttgggaaaaa
    16681 tgtaacggcg cttaaccagt ttaagcggtg catgtacaaa cacattgata gcatgaaaaa
    16741 tatattctcc tcttgacgtg ggacgtaatg catactccag gtcaaattca gcgccgcttc
    16801 ctaactgaac tctttttagc cagtttctct cctggaactg caccggcagt tcatcaatta
    16861 tactggcacg aacattaaat gcatactgat tgaccaggtt aataacaact ttattagaat
    16921 cgccaatact gaaccgttca gataccaacc gttctgccct tatcccgttt cttttactgt
    16981 aaacaagtaa tgcatcaata aaaattgcga tagctaataa aacaaggatc agcacgccta
    17041 cctgaaacaa ggccgggaaa aaataactta atgcaaacac aaccgctgct gaaccagcga
    17101 tatagtaaac aatattgttc aggtagaatg attttatcac ttatgcttat ttcagtttta
    17161 caattaaacc ttacctaggc acatccattc cctggattat ttgtttaatt acatcatcct
    17221 cactgatacc ttccatttct ttatctgggg ccagaatgat acgatgacga agcaccggag
    17281 ttaccacaga aagaatatca tcaggcgtta caaagtcacg gccgttcatg gctgccatgg
    17341 cttttgaggc attcataatt gccagggaag cccttggaga tgctccgaga taaattgatt
    17401 tatgatttct tgtctgatgt atcagtttcg caatgaactg taggagtttt tcttctacaa
    17461 tctgtgtctt tatctgttgc cgtaaagctg tcaccctggc tgcatccaaa gccggttgta
    17521 tcatatccat agccgaagta ttgcccatgc tatggaactg ggtgaggata ctcatttcct
    17581 ctgactctgt cgggtacggc actactatct taaacagaaa tcgatctaat tgcgcttcag
    17641 gcaggcgata agttccctcc tgctccacag ggttctgtgt agccagtacc ataaatggtg
    17701 ccgccattaa ataggtcttc ccatccactg tagcttgtct ttcctccatg atctccagca
    17761 gggctgattg agtttttgca ggagcccggt tgatttcatc cactaagaca atattgctga
    17821 atacaggtcc ctttttaaat tcaaaactgg cttcctttgg attgaaaacc ggtgttccca
    17881 agacatccga aggcattaga tcaggagtga actggatacg ggaaaatccg acgttgacac
    17941 tttttgcaac taatttggca gttagtgttt tggcaacacc cggtacccct tcaatcaaca
    18001 catggccatc ggccagtaag gcagcaataa tcagttttac catttcatcc tgtccaacaa
    18061 tgatcttttt aatttcattg cggatagata aaacagcatc attcaattca gacaggtcgg
    18121 tacgttgatg aaaaatttgt tcgtccatat ttatgctttt tgatagaata attccatttg
    18181 tttatgaaag tgagcaagct gctcctgact cactacggct gcctgatcaa gatatttaat
    18241 aaatgagata atttccccga tttctttctc atccactccg gacttataat gaagattcct
    18301 gacaaagtca tcatccaacc tgcttgtggg taatttatat ttactccgta catgttccag
    18361 gaaataggct gacattttat gacaaagatt tttgtgatcc cctttatcat aatacaaacg
    18421 accaatggtt tttacaaaat caagtgagtc attccggggt tttttcagca caggtatata
    18481 gcgctgctta cgtctcatct ccatcaaaac ataaaacaga agagtgaaaa ttgctgttaa
    18541 tagcgccgct ttgaaagcag ggtaacggaa caaaacactt aaccatccct catttttggg
    18601 cttgttaacc tgttctcctt ttttatacag gtaatactca tcccaaacaa tcttttttac
    18661 tccggggttg attactgaca atgctttttc ataataactg atattatcct tgtgcagtaa
    18721 aaaataatta cagaaggcca acggctccag gtgaacataa aaattaccct tcccggttct
    18781 caggtgaatg aaattaggtc tgccagattc atcgtatcct aatatttctg tagtcgatga
    18841 gtcaattgtt gaaaaataag aaccaaatgt ctttccggga taccggtaac gtaaagtgtc
    18901 accgaacggg ggatcgttga gtgaaaacct tgccccttca tccagatcac tgacggaaaa
    18961 aaaggcaaga tcataagcac tgctgccaca cccaaatgct tcatctgctg ccgaggaaat
    19021 atacctggcg ctgataaaaa tatcattccc aacccgggca aatgcaataa gctttctcat
    19081 ttcatcgtca tctgcactga acctgtctgt tataaccaag taagcctgcc tagagtcgta
    19141 catagaaaca gaatcccagt aaccgggttc aaggcgggat gtcctgatgg ttgctttcgg
    19201 gaaaagagat tcaaggttat tatatgccac ataggtagca tatggaatct tatcctgctt
    19261 acgtaatgta attctttcat ccaggcgacg gttcttataa ttatcaccag taataaaaag
    19321 aagtataatg gctgtcgcca gcaataaggc aagtaaatat ggtgtgtatc ttttcaacct
    19381 ttttatatcc tgttttcaaa ttgttcaaaa tcatttctta taaactggta tgcatcctgg
    19441 cttacttcaa atttcccata ccagctatat tcataattac gggtaatacg gaaaaagtct
    19501 ttgtaataag ctgtactatg taactgcatc aggtaatcaa gattcgtctt atcctgctgg
    19561 tagcggataa tatttctttc cgccatattt tttaataacc gcaaaaacat aagacggaca
    19621 gccatcctga aattccccat tgatgctgcc ttatcaattt ccttttcgta attaatggta
    19681 aaaatatccc cgggcatttc ctcatctact gctacaacag ctactttttt atttcgccgg
    19741 aataatgaga tattgctatc agccaggtaa tacatgatac ctgctgcaaa tacgccgatg
    19801 atcactaacc atactattgt tttaaaccag gtttgctgac caatcggtat gtatggagat
    19861 acagttgagg tttttttttc ttttttcttt tcaaattctg cgtcggcata ccaaaaatca
    19921 tcatccgctt tcattttcct gacgagactg tcggccagct tacgtacctg taatgaaaag
    19981 gtatcctcgt ttgcaagttc tagaaaatcc ggcttatcag cctccccgtc ttcttcatca
    20041 tattcttcct caacaggagg ctcctgaata acctcctcta ttattgcagt agtatcgctg
    20101 taatcctgtg cctgcataat taaatcacag cacagtagta tgcaaaccag tattgccctg
    20161 gtcttaaaaa atttaaaaat atttttaacg gggttctgct gcacttatac ttcctcctca
    20221 tttaattgca ctgataattt tttgtgaagc cttatcgggt aaagtatgaa ataccaaacg
    20281 acaaatatga cagataaggc aaagataata gtggtcagca ctgacacatc at
//
LOCUS       DAORTI010000058        20212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2270_length_20212_cov_7.375949, whole genome shotgun sequence.
ACCESSION   DAORTI010000058 DAORTI010000000
VERSION     DAORTI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20212
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_20212_cov_7.375949"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1101)
                     /locus_tag="PLO70_08995"
     CDS             complement(193..1101)
                     /locus_tag="PLO70_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQZ74642.1"
                     /translation="MIKIPPYLQKGDTVGLVCPAGYMTLDKVQTCIATLQEWGYKVKV
                     GKTVGGDSQTYFSGTDEERLNDFQLMLDDDEIKTVLCARGGYGMGRIIDKIDFKKFKK
                     QPKWIVGYSDITVLHSHLYSNYYISSLHSPMAGAFNEEGFKNEFVLSLKNALEGKKIK
                     YQCEAHEYNRKGEAVGELVGGNLALLAHLVGTDSDIKTRGKILFIEDVGEYLYNIDRM
                     MHQLKRSGKIARLAGLIFGGFTEVKDTDRPFGNTVLEILKDIVKEYSYPVCFDFPVSH
                     TDRNYALKVGVGYKLKVGKTKVTLEE"
     gene            complement(1098..2210)
                     /locus_tag="PLO70_09000"
     CDS             complement(1098..2210)
                     /locus_tag="PLO70_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HQZ74643.1"
                     /translation="MRKLMLLLCIASLQEARANDGVFYATGNTLIPLKETSIRLKKEI
                     LNLERKGDWMQVDIYFEFFNPGPEKELTVGFVTPPAEGDVGEEDARHPYIKDFMVMVN
                     DNLLPYRVAQLKETGFKVDLKVASGYDFVYYFKIKFLKGLTVIRHSYLYRGGASVEAK
                     VDFDYRLTTGTTWANSAIEDFELNINFGDDSYFAVPAGFGPTTVNWTVIGIGRLSTPV
                     NFNPYGEIGGGAKLRMAYLRKGKLQMRTTNFKPQKDLSLIVFQLHNEVNLWCDKRVKN
                     DFAGIMELVWGDSVELEVTKLSDLELRLYRNLNYARMGYDFKEEALKILFSKYNWYIP
                     DPAVKPEVVPDYYVTKELQQLILVEEKRRKIKTDTL"
     gene            2317..4428
                     /gene="metG"
                     /locus_tag="PLO70_09005"
     CDS             2317..4428
                     /gene="metG"
                     /locus_tag="PLO70_09005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQZ74644.1"
                     /translation="MIPPKRYLVTSALPYANGLKHVGHLAGAYIPADIYVRYLRAQKR
                     DVVFVCGSDEHGTAIPIQATKEGTTAQAIIDKYHEAMKQDFEDLDMSFDIYHRTSEPI
                     HHETAQEFFTKLNDAGELETKETEQYFDEEANTFLADRYIKGTCPNCGSDRAFGDQCE
                     TCGRTLSPDELINPVSTLSGKTPIKKKTTHWYLPLGKHEQFLREWILEQHKDDWRTTV
                     VGQCKSWIEGGLQSRAVTRDLDWGVKVPLKEAEGKVLYVWFDAPIGYISATKQWAIDN
                     KKEWKPYWQDKDTKLVHFVGKDNIVFHCIIFPIMLKLHGDILPENVPANEFLNLEGDK
                     MSTSRNWKLDMRDYINDFVKKENGGGQCVDMLRYYLTQIAPETKDSEFMWKGFQDAVN
                     SELVSIFGNFVNRTWVLMHKLCGGKVPPLHTGILDDNDNILIAEIGNAKTRIESLLEQ
                     YKFRDALYEVIDLSRKGNQYMQKKEPWIVAKKLTENPEVQKSIDNTMHLCLQLTANLA
                     IFINPFLPNTAKSMLHMMKVVDKLLDWENAGKLKLLSIGYSLREPQLLFRKIEDTEIT
                     EQIEKLKSGLKKMEEPKKDDKKVEAVFVKQEIQFDDFSRIDLKVGTIVSAEKVEKADK
                     LLKLSVDLGFETRTIVSGIALHFKPEDIVGKQVTVVTNLAPRKMRGIESNGMILMAED
                     KSGKLHFINPENLIDTGSNVS"
     gene            4555..8409
                     /locus_tag="PLO70_09010"
     CDS             4555..8409
                     /locus_tag="PLO70_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74645.1"
                     /translation="MKILKFSGIFLGAITILLIGFHFWINYNARHIIEDLVDTKSKGK
                     LKLKVEKFRFSWFSRKVELQNAYIYSTDSVNTSTSYRFRVANIKLTVQEILPIILSKK
                     LFIDSLNLEKPEITVTRLKAFTERDRSLKKEVSIPEELGKIYNSIQDALQVLKVNRFQ
                     ITNAKFTLVNKIQPGQLPLTISNIDFFIDNLSVDTARLSGKEKIFFSENVVLRSRDQD
                     IVFPDGRHRLSYSQFRVNLKKQLVEFDSCTIAAVQKDSTTSAFRVFFDTLRLTNIDFD
                     TLYRSELIKADSVYCLNPKFILEVELGKKSEGGKRTVPKLGDIIKQLTGNLQLGFVVV
                     DNADFNIKTINDEKPTSFAFTNNNFELQGLSVDQDAAKPLKVKSLALAIRNYENFIKD
                     SSYNVKFDSILFREDKIYLSHFIFNKLDNGKIINTFSIPQFRLDGLSWDDLVFKRQLK
                     ANAATLYQPSINYKVSVKKTTKKGRKNIFAVLGDIGQMIQLDKMNIVNGRVDLALNNN
                     SSVHLEDASIIIQTQRLLTAKKASALEHAVEYLDFKTGTIKTNNLDIKLYDVRYIGDK
                     NGYLLAKRVQLNERLSRTMIMAENAAVDEILIDEETSDLTATGIRWDKADVKVVLPEK
                     GEMENTNNAAIELKNIKGSNTTAIIKTGNITISTHLDNLSLDELLKEPGKEMQLKNLA
                     TAGKQLKIVDTFSSLTVSDYVIADGKSSILQDVSYYNKKNNNVISLSLPSIATTPDIN
                     SILNGKIKLSSLNLIRPVASLSLSTIHLPGEEKGSKMPEVDIDKIIILQPVINFNQHT
                     EKGNLNINWDGSIEKDNRLELTNILVNAGNFTDITVGKVLLNLNHFSYSEPKGKTFHT
                     GDGEVATEISAIKWQQESGKKPAWRAKVDDFTARNFHFDSLGKNHGILVINKTGLKGL
                     SISSSSINNLQKLAGANSSFQLQQFTGNYKDDKTEFRWFNAGFNQNSKIFTLDSFSFN
                     PTLSRESFLSKLTFQKDYINGGTGAIQIGPIDIDKYSKDMVLDIDCIKVDNPYLNDYK
                     DKSLPFAAGTIKPLPANMIKKIPFQISVDTIQLNNAKVDYTEVSEKTKMPGTIPVTRL
                     AVNLFNIKNYNLTATDSLSIHAYGYVMDTVWVRLRVKESYTDSLGGFLMTVRLKPGDV
                     TVLNPVLIPLASVKLISGQFDTINMRAVGREYLSLGEMKMFYKDLKFQLLRDGKVEKK
                     SFFTRMITFVATSFVIRKNNNSRTGNVFFIRQRDKSAINYLIKIAFSGMASSIGVKNN
                     KKMIRKYRNELEKRGLPPIEFE"
     gene            8487..10874
                     /locus_tag="PLO70_09015"
     CDS             8487..10874
                     /locus_tag="PLO70_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HQZ74646.1"
                     /translation="MSKRTIKKVAVLGSGVMGSRIACHFAGIGVQVLLLDIAAPPPPE
                     GEISKAPDKNKIVNDALTAAIKSNPSPVYHKDVIKKITTGNFDDNMKDIAGCDWVIEV
                     VVERLDIKQKVFEQVEKYRKPGTLITSNTSGIPIHMMAEGRTEDFKKHFCGTHFFNPP
                     RYLRLLEIIPTPYTDPEIVDFLMNYGDLYLGKTTVLCKDTPAFIANRIGVFGMMAIMN
                     VKEKLQLSIDEIDALTGPIIGRPKSATFRTADVVGIDTLVKVAKGVADNCPNDEAREQ
                     FDIPAWLDKMVANNWLGDKTGQGFFKKTKGPGGEKEILTLNLDTMEYGARIKPKFATL
                     EAAKPIDDLYSRLKMLVDGQDKAGEFYRLFHYGLFSYISHRIPEISDELYRVDDGMMA
                     GFGWEIGAFESWDVLGVTKTTEAMKAAGFKVAPWIDEMLAAGNKSFYRIEGGKKYCYD
                     PASKTYKMLPGGEAFIVMSNYTDKQIWKNGSCRLYDLGDDVLGLQWFTKMGSIGGDVL
                     AGMQTAIDKAEKNYKGLVIANEGANFSAGANVGMIFMLAIDQEYDELDMAIRMFQNTM
                     MRARYSSIPVVTAPHGLALGGACELSLHSDKCCPAAETYTGLVELGVGLIPGGGGTKE
                     FVLRASDEMHEDEPETITLKNRFLSIATAKVATSAHEGFGLGIYRKGLDEVVMNQGRR
                     IAEAKKAVIEIFDSGYTTPVQRKDIKVLGQTALGALYSGIHAMRTANYATEHDSVVAK
                     KLAYVMCGGDLSEPTLVSEQYLLDLEREAFLSLCGEKKTLERIQSVLKSGRPVRN"
     gene            10925..11287
                     /locus_tag="PLO70_09020"
     CDS             10925..11287
                     /locus_tag="PLO70_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74647.1"
                     /translation="MQITLVDFANDLVVYNITGISREELENKLNLFFAAENLPLKSDK
                     PDEKVYQKGSKIMRIIFGVFVKYFKVVVSIKQQDELFSVKIVRDMNILLSGGLIGINK
                     SRKEFTRLTEAFKVYFKD"
     gene            11371..13575
                     /locus_tag="PLO70_09025"
     CDS             11371..13575
                     /locus_tag="PLO70_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQZ74648.1"
                     /translation="MKKIFTAILLLLIQQCIFAQDKLLSMEDAMLKARTSLAPQNLSQ
                     LQFYGNTNDYVYLKKVEGKDIWMRGNFKMKADSAFLTLDKLNNLLSQNGLTTAKEMPL
                     IKFTPKGWIMTSNGHKILFDPEKRSYVTLLKKELAAKENAEESSIGYVAYLDNHNLYV
                     TDGTIAKQVTTDGSKNIVYASSVHRDEFGINKGTFWSNNGTKLAFYRMDQGMVTDYPI
                     INWSERPAKADLIKYPMAGDKSHHVTIGVYNTATQNTIWLKTGEPAEQYLTNIAWSPD
                     DKYIYIAVVNREQNHMWLNQYDATTGDFIKTLFEQTDEKYVEPLVPIQFLKSNPSQFI
                     WQSNRDGWNHLYLYDTNGKLLKQLTNGNWEVLEVKGYDAKGENLYFVSTALSPINKNL
                     SVLNLKTGRINLITTDGGVHATQVSADGSSILDVYSTASNPRTIQLIDISKFLNAKAK
                     VQQKGLQNLVAKPKLLMQSHNPLKEYATGELSLFTIRNNSGDSIYCRMFKPVGFNNTK
                     KYPVIVYWYGGPHAQMILNSWNGGAGDYWFQYQAERGYVVFTIDTRGSDNRGKAFEQS
                     IFRKLGEAQMDDMMAAVNYLTAQPYIDKDRMGLFGWSFGGFVTTDFMLNHPGIFKAAV
                     AGGPVMNWQYYEIMYGERYMDTPQENPEGYAATNLIKQAGKLKGKLLLIHGLQDPVVV
                     QQHSVDFVKACVDKGVQVDYMIYPGHEHNVLGKDRAHLYQKVTDYFMQHLQQ"
     gene            13591..14271
                     /locus_tag="PLO70_09030"
     CDS             13591..14271
                     /locus_tag="PLO70_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74649.1"
                     /translation="MKKIIIYIAGCLLGLSTSAQVNYSGAYGYTFPPIGDSPKNENAK
                     GASGSLVLLKMENNKYRFWLDVTIGWPSYNMGETDGTITFVNDTASFDNSFEDASAPC
                     ILKFRIKGDVITIDSKSSSFNCGFGNAVNADGDYTRLKNQPVFNNDWLKKEYNQSPTI
                     VITADKATVYEDENCIYAKKQYFIKGDKLLSIAETEKTMYVEYIPTPGKFVYGWIKKT
                     MTKETSSE"
     gene            14277..14945
                     /locus_tag="PLO70_09035"
     CDS             14277..14945
                     /locus_tag="PLO70_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HQZ74650.1"
                     /translation="MGTVETGSQQIFINSHIITYGNAANEALTEMIRDEIETMWNEPQ
                     ARVVFNDYDCRIVFSITAAFEPGIRDIDIYQNLDPRNNYFRIEEFAYGNISFVDGLGC
                     NSGYFKLENLYKGSTTAAHEYGHTIGLNHPDELDIRGKGVPGIMYPRGTLVDPQYQYE
                     PDKPAGTKGGTLHPMYRRVLQEDIDKLKLNKLNFKNGKTILGEFTNIWHPNHSETDFS
                     NYMA"
     gene            complement(14950..15879)
                     /locus_tag="PLO70_09040"
     CDS             complement(14950..15879)
                     /locus_tag="PLO70_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HQZ74651.1"
                     /translation="MATILLTGGTGLVGRALSSALLEKKHNVIILTRSTKQMAISPQP
                     KNGIQWVQWNVEAQTIDKDAVAKADYIIHLAGAGVADKRWTKKRKQEIVDSRVKSGAL
                     LVTALSEIENNVKAVITASAIGWYGADPVIPNPIPFLEDAPADTAFLGDTCRQWEQSI
                     EPVTQLKKRVVKLRIGIVLSKDGGALKEFIKPLQFGVAAILGNGKQMISWIHIDDLVN
                     MFMAAIENENMNGVYNAVAPKPVSNKELTLELAISRKKFFIPFYVPSFILKLLLGEMS
                     IEILKSATVSSTKIQQAGFNFKFPDIKSALKNN"
     gene            15986..16882
                     /locus_tag="PLO70_09045"
     CDS             15986..16882
                     /locus_tag="PLO70_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ74652.1"
                     /translation="MSIVLSIKNIAKSYGSVRALNGVSFDVPQGSVFGILGPNGSGKT
                     TLLGIVMDVLKANTGDFDWFGQPGSPEQRKKIGSLLETPNFYSYMSAIDNLKITQSIS
                     GRGTSADIDAVLKKVNLYDRRRYRFNSYSLGMKQRLAIGAALLGNPKVLVLDEPTNGL
                     DPVGIAEIRQLIIELRDQGHTIIMASHLLDEVEKVCTHVAILKSGNLLTTGNVEEVMM
                     DEDIVELSAADLTALTALFRKREQQIIFDERANTLQVILPKGTAKMEDINRLCFENGI
                     VLNHLILKKKRLEARFFELTNN"
     gene            16899..17720
                     /locus_tag="PLO70_09050"
     CDS             16899..17720
                     /locus_tag="PLO70_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ74653.1"
                     /translation="MIKLIKTEWLKMRKYTAFWWIMGLTALAYPGINYAFYMVYDNLM
                     NQPTQASKMAKMLIGNPFTFPEVWHTVAYFSSWFVFIPAVVVIMFISNEYSFKTHRQN
                     IIDGWSRKQFITSKLMDVMIISLLISVLYIIVSMVSGYASKSEGSGEMWDKSYFIGLF
                     TLQTFAQLSIAFLIGFLVKKAFIALGIFIFYFLILENILVGLFKVKVNDIGRYLPFEI
                     SDRMIPVPGFLGKLESAENYQKAFDDITLHVILTLILSVIIWGICYWINSKKDLK"
     gene            complement(17748..19007)
                     /locus_tag="PLO70_09055"
     CDS             complement(17748..19007)
                     /locus_tag="PLO70_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQZ74654.1"
                     /translation="MAFTKLSLIEPILKALSTEGYTIPTPIQQQAIPLVLQRKDLLGC
                     AQTGTGKTAAFAIPILQILYEQKPLAAGIKVLILTPTRELAIQIDESFAAYGRFTGIR
                     HTVIFGGVSQQNQTNALRRGVDVLVATPGRLLDLMSQGYIHLDKLQIFVLDEADRMLD
                     MGFIHDVKKVIAKLPAKRQTLFFSATMPPEIQKLAAVLLTHPAKVEVTPPSSTVDKIE
                     QSLYHVGKMEKPELLLHLLGNKEIKTALVFTRTKHGADKVVKILQRHNIRAAAIHGNK
                     SQNARQGALSDFKNGTLRVLVATDIAARGIDIDELTHVINFELPNIPETYVHRIGRTG
                     RAGNIGTAISFCSTEERAELKDIQKLISKTIPVVHEHPFTANAHIAAPAPATRQQQSF
                     KPRRNFNTAAGQGGNSQRRSYGAQRSR"
     gene            complement(19266..19571)
                     /locus_tag="PLO70_09060"
     CDS             complement(19266..19571)
                     /locus_tag="PLO70_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74655.1"
                     /translation="MLPDNELQQHWFQLEYALTERLGKKLTLEDILLFIGVREAGIPP
                     KEFTEREKIELMQVAISTILVPAKYYELLWVEDTGWPHFKQLQPIKEMNAKDKEFFL"
     gene            complement(19894..>20212)
                     /locus_tag="PLO70_09065"
     CDS             complement(19894..>20212)
                     /locus_tag="PLO70_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQZ74656.1"
                     /translation="PMAHQAQSPLSTEVEDSALQDETEEQKRRAAERLHKLRNLSFNV
                     NAADPNNEFETVPAYIRRNMELYNSNTQVENFYSSYEVKSDDKNQGHISTINTFLDGK
                     KPD"
BASE COUNT         6482 a         3950 c         4087 g         5693 t
ORIGIN      
        1 aatctgtacc ggtacaaaga aaccgtgaat aaacttgact ctgttttaag gctataacta
       61 aaccagcaca aggattcggc aataatcctg aaccagcact aactaagcag ctttcaaatg
      121 aatcactgtt aacggtataa tttttttgac ccggttttaa tggttgcaac cttccgtttg
      181 gcgagtcagc ttttattctt ccagagttac ttttgttttt ccaactttca gcttatatcc
      241 cactcccact tttagcgcat aatttctgtc tgtatggcta accggaaaat caaaacaaac
      301 cggataactg tactctttaa ctatgtcctt tagtatctca agaacagtat taccgaaagg
      361 tctgtctgta tcttttactt ctgtaaaacc gccaaaaatc aagccggcaa gccttgctat
      421 cttgccactt ctttttaact ggtgcatcat cctgtcaata ttatataaat actcccctac
      481 atcttctata aaaagaattt tacctcttgt cttaatgtct gaatctgtgc ctaccaaatg
      541 ggcaagcaag gcaagattcc caccaactaa ctctcctaca gcttcacctt tcctgttata
      601 ttcatgtgct tcgcattgat atttaatctt ctttccttcg agtgcatttt ttaaggaaag
      661 aacaaattca tttttaaaac cctcctcatt gaaagctcct gccataggag agtgaagtga
      721 tgaaatatag taattagaat aaagatgaga atgcaacaca gtgatatcgc tatatccaac
      781 gatccacttt ggttgctttt taaatttctt aaaatcgatt ttatcaataa tacgtcccat
      841 tccataacca ccccttgcac ataaaaccgt ttttatttca tcatcatcca gcattagctg
      901 aaagtcgttg agtctctctt cgtcagtacc ggaaaaataa gtctgagaat caccaccgac
      961 tgttttcccg actttaacct tatagcccca ttcctgcaaa gttgcaatgc aggtctgtac
     1021 tttatccagg gtcatataac cggcaggaca aaccaatccg accgtgtcgc ccttttgcaa
     1081 ataaggtggt attttaatca tagagtatct gttttaattt ttctgcgttt ttcttcaact
     1141 aaaattaatt gttgcaattc ttttgttaca taatagtcag gtacaacttc tggctttact
     1201 gccgggtccg gaatatacca gttgtatttt gaaaataata ttttcaacgc ctcctctttg
     1261 aaatcataac ccatgcgggc ataattcagg tttcggtata atctcagttc aaggtctgaa
     1321 agtttcgtta cctccaactc gaccgaatct ccccaaacaa gttccataat accagcgaaa
     1381 tcgtttttca ctcttttatc acaccacagg tttacctcat tgtgcaactg aaaaacaatc
     1441 aatgataaat ctttttgggg cttaaagtta gttgtcctca tttgtaattt tccttttcgc
     1501 aggtaagcca ttcgtaactt cgctcctcct cctatttcac cgtatggatt aaaattaacc
     1561 ggagtactga gccggccaat tccgattact gtccagttga cagttgttgg cccgaaacca
     1621 gcaggaacag caaaataact gtcatcacca aaattgatat tcagctcaaa atcttcaatg
     1681 gcactgttgg cccaggtggt ccctgtggta agacggtaat caaaatcaac tttggcttca
     1741 actgaagccc cacccctgta taaataacta tgccggatca ccgttagtcc ttttagaaat
     1801 tttattttaa aatagtacac aaaatcatat ccactggcaa cttttaaatc cactttaaaa
     1861 ccagtttctt tcagttgggc cacccggtat ggcaacagat tgtcattcac catcaccatg
     1921 aaatccttta tatatggatg cctcgcatct tcttctccaa catcaccttc ggcaggtgga
     1981 gttacaaatc caaccgtcag ttctttttcc ggacccggat taaaaaattc aaaatatata
     2041 tccacctgca tccaatctcc tttccgctcc aggttcagta tttccttctt taaacgaatt
     2101 gatgtttcct tcaaaggaat taatgtatta cctgtagcgt aaaatacacc atcgtttgcc
     2161 cgggcctctt gcaaactggc tatacaaaga agtaacatta gttttctcat aatgagaccg
     2221 gtttaaggtt aatgcattaa aggtatcgga aatcgtcctt ttgggcaatg accgctattc
     2281 cggtattttt gcaggttaat aagacttaat ttataaatga tacctccaaa aagatattta
     2341 gttacttctg cccttcccta tgccaacggt ttgaaacatg taggccacct ggcaggcgcc
     2401 tatataccgg cagatatcta tgtccgttac ctccgggcac aaaaaagaga tgtagtattt
     2461 gtgtgcggca gcgatgaaca tggtactgcc attcctattc aagccaccaa agaaggaacc
     2521 acagcacagg ctattattga taaatatcac gaagcaatga agcaggattt tgaagacctg
     2581 gatatgagct ttgatattta tcaccggacc agcgaaccta ttcaccacga aacagcacag
     2641 gaatttttta ccaaactgaa tgatgccgga gaactggaaa caaaagaaac tgagcagtat
     2701 tttgatgagg aagccaatac cttcctggct gaccgttata ttaaaggcac ctgtcctaac
     2761 tgtggcagtg accgggcttt tggtgatcag tgtgaaacct gtggccgtac cttaagtcct
     2821 gatgaattga ttaacccggt gagtactttg agcgggaaaa caccaattaa gaaaaaaaca
     2881 actcactggt atttaccact gggaaaacat gaacagtttt tgagagaatg gatattagaa
     2941 caacataaag acgactggag aactactgtt gttggtcaat gtaaaagctg gatagaaggc
     3001 ggcctgcaat caagagccgt aacaagagat cttgactggg gggtgaaggt tccattaaaa
     3061 gaagctgaag gcaaagtttt gtatgtatgg tttgacgccc ccattggtta catcagcgct
     3121 actaaacaat gggctataga taataaaaag gaatggaaac cctactggca ggataaagac
     3181 acaaaattgg tacactttgt tggcaaagac aatattgtgt tccattgtat catcttcccc
     3241 atcatgctga aattgcatgg tgatatttta cccgaaaatg ttcccgccaa tgagttcctg
     3301 aacctggaag gcgacaagat gagcaccagc cgaaactgga agctcgatat gcgggactat
     3361 atcaatgatt ttgtaaaaaa agaaaatggt ggcggccagt gtgtggatat gcttcggtat
     3421 tacctgaccc agatcgcccc ggaaacaaag gacagtgaat ttatgtggaa aggtttccag
     3481 gacgctgtta acagtgaact ggtttccatc tttggcaatt ttgtaaaccg gacctgggta
     3541 ttaatgcaca aactctgcgg aggcaaagta ccaccattgc atacaggtat cctggatgat
     3601 aatgacaaca tactgatagc agaaatcgga aatgcaaaaa caagaattga atctttatta
     3661 gaacaatata agttcaggga tgccttatat gaagtgatcg atctgtcaag gaaaggcaac
     3721 cagtatatgc aaaagaaaga gccctggatt gttgccaaaa aactgaccga aaatccggaa
     3781 gttcaaaaaa gcattgacaa cacaatgcac ctgtgtttac agcttacagc caacctggcc
     3841 atttttatca accctttttt acctaacact gccaaaagta tgctgcatat gatgaaagtg
     3901 gtagataaat tactggactg ggaaaatgca ggcaagctta agttattgag tataggttat
     3961 agtttaagag agcctcaatt attattcaga aaaattgagg acacagaaat aacagaacaa
     4021 attgaaaaat taaaaagtgg gctaaaaaaa atggaagaac cgaagaaaga tgacaaaaaa
     4081 gtggaagcag tattcgtaaa gcaggagata caatttgatg atttctccag gattgacctt
     4141 aaagttggaa caattgtatc ggccgaaaaa gtggaaaagg ctgacaagct tctcaaatta
     4201 tctgttgatc ttggttttga aacaagaacc attgtcagtg gtattgcctt acatttcaaa
     4261 cccgaggata ttgtgggcaa acaagtaacg gttgtaacca acctggctcc gagaaaaatg
     4321 cgaggtattg aaagcaacgg aatgatcctg atggcagagg ataaatcggg aaaactgcat
     4381 tttattaacc ccgaaaattt gattgatacc ggttcaaatg ttagctaacc ggtaaaatta
     4441 cacaaacccc cgttttatct gaactgaaac gggggctttc attttataag cggggagatt
     4501 tcactaaatt catgcttaaa tctcatccgc accttgacaa caaagcgtct tggcatgaaa
     4561 attctgaaat tttccgggat attcctcggt gccattacta ttcttttaat tggttttcac
     4621 ttctggatca actataatgc ccgtcatata atagaagacc ttgtagatac aaaatcaaaa
     4681 ggtaaactaa aactaaaagt tgaaaaattc aggtttagct ggttcagccg gaaagttgag
     4741 ctacaaaatg cctatatata ttctactgat tcagtaaaca ccagtacctc ttaccggttc
     4801 cgggttgcta atattaaact tactgtacag gaaatcttac caattatttt aagtaaaaaa
     4861 ttatttattg attcgctgaa cctggagaaa cccgaaataa ccgttacccg gttgaaggct
     4921 tttacagaaa gagacagatc actgaaaaag gaagtttcta tacctgaaga attaggaaag
     4981 atatataact ctatacagga tgctttgcaa gtattaaaag taaaccgttt tcagataacc
     5041 aacgcaaaat tcactctggt taataaaata caacctggtc agctaccact caccataagt
     5101 aatattgatt ttttcattga caatctttcg gtagatacgg ccagactatc cgggaaggaa
     5161 aaaatatttt ttagcgaaaa tgtggtatta agaagcaggg accaggatat tgttttccct
     5221 gacgggagac accggcttag ctatagccag ttccgtgtca atctcaaaaa acaattggta
     5281 gagtttgaca gctgtactat tgctgcagtc cagaaagaca gtactacatc agcattccgt
     5341 gtattcttcg atacactcag actcacaaat attgactttg ataccctata ccggagtgaa
     5401 cttattaagg ccgattcagt ctactgcctt aacccaaaat tcattcttga agtagagtta
     5461 ggtaaaaaaa gtgaaggagg caaaaggaca gttccaaaac tgggagatat tatcaagcaa
     5521 ttaaccggca accttcaact aggctttgta gttgtggata atgctgattt caatatcaaa
     5581 actatcaatg atgaaaagcc cacttccttt gcgtttacca ataataattt tgaactgcag
     5641 gggctaagcg ttgaccagga cgcagcgaaa ccactaaaag taaaaagcct tgctttagcc
     5701 atccgcaatt atgaaaactt cattaaagac agttcctata atgtaaagtt tgacagcatc
     5761 ctgttccggg aagataaaat ttatttaagc cacttcattt tcaataaact cgacaatggt
     5821 aaaatcataa atacattcag tattccccaa ttccggctag atggactttc ctgggatgat
     5881 ctcgtcttca aaagacaatt gaaagcaaat gccgcaacgc tttatcagcc ttccataaac
     5941 tataaagtgt ccgtaaaaaa aacaacaaag aaagggagga aaaatatttt cgccgtcctt
     6001 ggagacattg gacaaatgat ccaactggat aagatgaata ttgtgaatgg gcgggttgat
     6061 ctggccttaa acaataatag ttctgtccac ctggaagacg cctccatcat tatacaaaca
     6121 cagagacttc tcactgcaaa aaaagcatca gcactggaac atgcagtaga atacctggat
     6181 ttcaaaacag gcaccattaa aacaaacaac ttagatatca aattatacga cgtaagatat
     6241 ataggtgata aaaacggata cttgctggca aaaagggttc aattaaatga aaggctaagt
     6301 cgtacgatga taatggctga gaatgctgct gttgatgaaa tccttataga tgaagaaaca
     6361 agtgatttaa ccgctaccgg tatccggtgg gataaagctg atgtaaaagt cgttttaccg
     6421 gaaaagggag aaatggaaaa tacaaataat gcagctatcg aattaaagaa tataaaagga
     6481 agcaatacaa cggcaattat taaaaccggc aacattacta tcagtactca tttggacaat
     6541 ttatcactgg atgaactact taaagaacca ggcaaggaaa tgcaacttaa aaacctggct
     6601 actgcaggaa agcaactgaa aatagttgat accttttcat cgttgaccgt gtcagattat
     6661 gtcatcgcag atggcaaaag ttcaatcctg caagatgtca gttattataa taaaaaaaat
     6721 aataatgtca ttagtttatc ccttccatcc attgcaacaa cgcctgatat aaactcaata
     6781 ctgaatggaa aaattaaact atccagctta aacttaatcc ggcctgtagc ttccttgtca
     6841 ctttcaacta tccatttacc cggagaagaa aagggatcaa aaatgcctga agtagatatt
     6901 gataaaataa ttatcctcca gccggtaata aatttcaatc agcatactga aaaggggaat
     6961 ctgaatataa actgggatgg aagtattgaa aaagacaatc gcctagaact aacaaatatc
     7021 cttgtcaacg ccggtaattt cactgatatt acggtgggta aagtgttgct gaatctgaat
     7081 catttcagct acagcgaacc caaagggaaa acctttcata caggtgacgg agaggtagca
     7141 acggaaataa gtgctattaa atggcaacag gagtcaggta aaaaaccggc atggagggcc
     7201 aaagtggatg attttacagc cagaaatttt cattttgaca gtttgggtaa aaatcatggt
     7261 atactggtaa ttaacaaaac agggttgaaa gggcttagta ttagttcatc ctcaatcaac
     7321 aatctgcaaa aactcgcagg agcaaattca tcatttcaac tacagcagtt tacaggtaac
     7381 tataaagatg acaaaactga attccgctgg ttcaatgcag gttttaacca gaattctaaa
     7441 atttttactc tcgactcctt ttcatttaat ccaacactga gcagagaatc atttctatca
     7501 aaactgactt tccagaaaga ttatatcaat ggtggtactg gtgctattca aattggcccg
     7561 attgatatcg acaaatatag taaggacatg gtgcttgata ttgactgtat aaaagtagat
     7621 aacccttact taaatgatta caaggataag agcctgccat ttgcagcagg aactatcaag
     7681 ccactgcctg caaacatgat caaaaaaatt cctttccaaa tatcagttga tacaattcag
     7741 ttgaacaacg ccaaagttga ctacacagag gtcagtgaaa aaacaaaaat gccaggcacc
     7801 attcctgtaa caagactggc cgtaaatctt ttcaatataa aaaattacaa ccttacagct
     7861 acagatagtc tcagcatcca tgcctatggt tatgtgatgg atacagtatg ggtcaggttg
     7921 agggtaaaag aatcctatac tgattcatta ggtggattcc tgatgacagt aagactaaaa
     7981 cctggagatg taactgtctt aaatccggtg ctgatcccgt tagcatctgt aaaattgatt
     8041 tccggacaat ttgatactat taacatgcgg gcggttggaa gagaatatct gtcgctgggc
     8101 gaaatgaaaa tgttttacaa agatttgaaa tttcagcttc tccgggatgg caaagtagaa
     8161 aagaagtcct tctttacaag aatgatcact tttgtagcca caagttttgt tatcaggaaa
     8221 aataataatt caagaacagg caatgttttc tttatccgtc agcgggataa atcggctatc
     8281 aactatctta tcaaaatagc ttttagcggt atggccagta gtattggggt gaagaataat
     8341 aaaaaaatga tacggaaata cagaaatgaa ttggagaagc gggggttacc gcctattgaa
     8401 tttgaataat gtaattaata cctgaaaaat cagaaaactc ttatcttcga tttccaaata
     8461 gttgcataac taactaattt tgctttatgt caaaaagaac tatcaaaaag gtagccgttt
     8521 taggaagcgg tgtaatgggt tcaagaattg cctgtcattt tgccgggatc ggcgtacagg
     8581 tattactatt agatattgcg gcccccccgc cccccgaagg ggagattagt aaagcccctg
     8641 ataaaaataa gattgtgaat gatgcattga ctgctgctat caaatcaaat ccgtccccgg
     8701 tttatcataa agacgttatt aaaaaaatca ccaccggcaa ctttgatgat aacatgaaag
     8761 acattgccgg ttgcgactgg gtaattgaag tagttgtaga aagactggat attaaacaaa
     8821 aagtattcga acaggtagaa aaatacagaa agcccggaac acttattact tccaatacat
     8881 ccgggattcc gattcacatg atggctgaag gcagaactga ggactttaaa aaacatttct
     8941 gtggtaccca ttttttcaat cccccacgtt atttaagatt actggaaatt attcccaccc
     9001 catatactga tccggaaata gttgacttcc tgatgaacta tggtgatttg tacctgggaa
     9061 aaacaacggt gttatgcaaa gacacaccgg cattcattgc caaccgcatt ggagtatttg
     9121 gcatgatggc catcatgaat gtaaaagaaa agctgcaact cagcatagat gaaattgatg
     9181 cactgaccgg gccgatcatc ggccgtccaa aatcagctac attcagaaca gctgatgtgg
     9241 ttggtattga cactctggtg aaagtggcta aaggtgttgc agataattgc cccaatgatg
     9301 aagcaagaga acaattcgat attcctgcct ggttagataa aatggtagcc aataactggc
     9361 tgggcgacaa aacagggcaa ggctttttca aaaagacaaa agggccgggt ggtgaaaaag
     9421 aaatattgac actgaacctt gatacgatgg aatatggcgc caggataaaa ccaaagttcg
     9481 ctacgctgga agctgctaaa ccaattgatg atttatactc caggttaaaa atgcttgtag
     9541 atggccagga taaggccggt gaattttatc gtctttttca ttatggcctg ttttcatata
     9601 tctctcaccg tattcctgaa ataagtgacg agttgtatcg tgtagatgat ggcatgatgg
     9661 ctggttttgg ctgggagatc ggtgcttttg aaagttggga tgttctgggt gttacaaaaa
     9721 caaccgaagc catgaaagca gccggtttta aagtagctcc ctggatagat gaaatgctgg
     9781 cagcaggaaa taaatcattc tacaggatag aaggtggtaa aaaatattgc tatgaccctg
     9841 cgtccaaaac atataaaatg cttccaggtg gtgaagcatt tatcgtaatg agcaattaca
     9901 ctgataagca gatctggaaa aatggaagtt gccggttata tgatctgggt gatgatgtac
     9961 tcggtctgca gtggttcaca aaaatgggaa gtattggcgg agatgtatta gctggtatgc
    10021 aaaccgctat tgacaaagca gaaaaaaatt ataaaggttt agtgattgcc aatgaagggg
    10081 ccaacttctc agccggtgct aatgtaggaa tgatcttcat gctggcgata gaccaggaat
    10141 atgatgaact ggatatggca atccgtatgt tccagaatac aatgatgcgg gcaagatatt
    10201 cttccatacc ggtagttact gctccacatg gcttagcact tggaggagcc tgtgagttga
    10261 gcttacattc ggacaaatgc tgcccggctg ctgaaacgta tactggtctt gttgaattag
    10321 gcgttggctt aataccagga ggcggaggta ccaaagaatt tgtattgaga gcttcggatg
    10381 aaatgcatga agatgaaccg gagacgatca cattaaagaa tcgtttcctt tcaatagcta
    10441 ctgcgaaagt tgctacttca gcacatgaag gttttggatt gggaatttac aggaaaggac
    10501 ttgatgaagt ggtaatgaac cagggaagaa gaattgcaga agccaaaaaa gcagtaattg
    10561 aaatatttga cagtggctat accacaccag tgcaacgaaa ggatataaaa gtattgggac
    10621 aaacagctct cggtgcttta tactccggaa ttcatgccat gcggacagcc aactatgcta
    10681 cagaacatga ttcagttgta gcaaaaaaat tagcatacgt aatgtgtggc ggcgacctga
    10741 gtgaacctac attagtatca gaacaatatc tgcttgactt agaaagagag gcattcctgt
    10801 cactatgtgg tgaaaagaaa accctggaga gaatacagag tgttttgaaa agcggaaggc
    10861 ctgtcagaaa ttaaagaaaa attgcagaga tcaccggggt caacccaact taaaaacttg
    10921 cactatgcaa ataacccttg ttgattttgc caatgacctg gtagtataca atatcactgg
    10981 catcagccgc gaagagttag aaaataaatt aaatcttttc tttgctgcag aaaaccttcc
    11041 tttaaaatca gacaaacctg atgaaaaagt ataccagaaa ggaagtaaga tcatgcggat
    11101 catttttggt gtctttgtga aatattttaa agtagttgta tctataaaac aacaagacga
    11161 acttttttct gtcaaaatag tcagggatat gaatatactt ctgtcgggag ggctgatagg
    11221 gatcaataaa tcacgcaaag aatttacccg gcttacagaa gcatttaaag tctatttcaa
    11281 agattaaact cattcttacg ttcagcaggc ccccgtaacg ggggctttta ttagccatta
    11341 tagctacatt tgtttatcta aaatcgattt atgaaaaaga tttttactgc tatactgctg
    11401 ctccttattc aacaatgcat attcgcccag gataaattgc tcagcatgga agacgctatg
    11461 ttgaaagcca ggacatcact ggcaccacaa aatctttcac agctgcaatt ctatggcaat
    11521 accaacgatt atgtgtacct caaaaaagtg gagggaaaag atatatggat gaggggaaat
    11581 tttaagatga aagcagattc agcattcctt acacttgata aactaaataa cctcttaagt
    11641 cagaacggtc ttactacggc aaaagaaatg cctctaataa aattcacccc aaaaggctgg
    11701 atcatgacat caaatggaca caaaattctg tttgatccgg aaaaaaggtc atatgtcaca
    11761 ttattaaaaa aagaattagc agcgaaggaa aatgcagaag aaagcagcat tggttatgtt
    11821 gcttatctgg ataatcataa tttgtatgtc acagatggta ctattgccaa acaggtaacc
    11881 accgatggga gtaaaaatat tgtctatgcc tcctcagtcc accgggatga atttggcatc
    11941 aataaaggaa cattctggag caataacgga acaaaactag ctttttacag aatggaccag
    12001 ggtatggtta ctgattatcc cattatcaac tggagtgaac ggccggcgaa agcagattta
    12061 ataaaatatc ccatggccgg cgataaaagc catcatgtga ccataggtgt ttataatact
    12121 gcaactcaaa atacaatatg gctcaaaaca ggtgaacccg ctgaacaata ccttactaat
    12181 attgcatgga gccctgatga caaatacatc tacattgctg ttgtaaacag ggaacaaaat
    12241 catatgtggc tgaatcagta tgatgcaaca accggtgatt tcatcaaaac cttatttgag
    12301 caaacagatg aaaagtatgt tgaaccgctg gtaccaatac aatttttgaa atcgaatcct
    12361 tcacaattta tctggcaaag caaccgcgac ggatggaacc atttatacct gtatgataca
    12421 aacggcaaac tgctgaaaca attaaccaac gggaactggg aagtgctgga agtaaaaggg
    12481 tatgatgcaa aaggagaaaa cctgtatttt gtatcaactg ccttatcacc aatcaataaa
    12541 aatctatctg tactgaattt aaaaacaggt cggatcaatt taataaccac tgatggcggt
    12601 gtacatgcta cccaggtcag tgcagatggg agctccatat tagatgtgta tagtactgca
    12661 tcgaacccaa gaacgattca actgattgac atcagtaaat ttctcaatgc aaaagctaaa
    12721 gtccagcaga aaggcctgca aaatcttgtc gccaaaccaa aactattaat gcagtcgcat
    12781 aacccgctga aagaatatgc aaccggtgaa ctttcattgt tcacaatacg caataatagt
    12841 ggcgacagca tttattgcag gatgttcaaa cctgttggtt tcaacaacac aaaaaaatac
    12901 ccggttattg tgtattggta cggcgggcct catgcacaaa tgatactgaa cagctggaat
    12961 ggaggtgccg gtgattactg gttccagtac caggcagaaa gaggatatgt tgtctttaca
    13021 attgatacca ggggaagtga taaccgcgga aaagcctttg aacaaagtat attcagaaaa
    13081 ttaggagaag cccagatgga tgatatgatg gcagcagtaa attatctgac tgctcaaccg
    13141 tatattgata aagaccggat gggattattt ggctggagtt ttggcggatt tgtgacaacg
    13201 gattttatgc tcaatcatcc cggcattttt aaagccgcag tagcaggagg cccggtaatg
    13261 aactggcaat attatgaaat aatgtatgga gaaagatata tggacacccc gcaggaaaat
    13321 cctgaaggat atgcagccac aaatcttatt aaacaggccg gaaaacttaa ggggaaatta
    13381 ttattgattc atggattgca ggacccggtc gttgtgcagc agcattccgt agattttgta
    13441 aaagcctgtg tagacaaagg agtacaggtg gattacatga tctatccggg tcatgaacat
    13501 aatgtgcttg gtaaagacag ggcacatctt taccagaaag tgactgatta tttcatgcag
    13561 catttacaac aataaatttt agcttccgct atgaaaaaaa tcatcattta tattgcagga
    13621 tgtctgctcg gactatccac ctcagcacag gtaaattatt cgggtgccta tggttatact
    13681 tttccaccga ttggtgattc gccaaagaat gaaaatgcca aaggggcatc cggctcactg
    13741 gtcttgctga aaatggaaaa taataaatac cggttctggc tggatgtaac aattggctgg
    13801 ccttcttata atatgggaga aacggacggc acaatcacat ttgtaaatga taccgcctca
    13861 ttcgacaata gttttgaaga tgcttctgct ccctgcatac tgaagttcag gatcaaagga
    13921 gatgtaatta ctatcgatag taaatccagt tcatttaatt gcggtttcgg gaatgcagta
    13981 aatgcagatg gtgattatac caggctgaag aatcaaccgg tattcaacaa cgactggctg
    14041 aagaaagaat ataatcagtc gcccactatt gtcattacag ccgataaggc aacagtttat
    14101 gaagacgaga actgcattta cgctaaaaaa caatatttta tcaaaggtga taagctcctc
    14161 tctattgcgg aaacagaaaa aacgatgtat gttgaataca tccctactcc cggtaaattc
    14221 gtctatggct ggataaagaa aacaatgact aaagaaacca gctccgaata aacaagatgg
    14281 gcacagttga aacaggtagt cagcagatat ttattaattc gcatataatc acttatggca
    14341 atgcggctaa tgaagcattg acagaaatga ttcgtgatga aattgaaacc atgtggaatg
    14401 aaccgcaggc aagagtagtc tttaatgatt atgattgcag aattgtcttt tctatcacag
    14461 cagcattcga gccaggaata agggatattg acatttacca gaacctggat ccccgtaaca
    14521 actatttccg aattgaagaa tttgcatacg gcaatatttc ttttgttgac ggattgggtt
    14581 gcaatagcgg ctatttcaaa ctggagaatc tatataaggg ttctaccact gccgctcatg
    14641 aatatggaca tacaataggt cttaatcatc cagatgaact tgatatccgg ggtaaaggag
    14701 ttcccggcat tatgtaccca agaggaacgt tggttgatcc gcaataccag tatgaaccgg
    14761 ataaaccagc cggtaccaaa ggtggcactc ttcaccctat gtacagaaga gttttacagg
    14821 aagacattga taaattgaaa ctgaacaagc tgaattttaa aaatggtaaa acaattttgg
    14881 gtgaattcac caatatttgg caccccaatc attcggaaac agacttcagt aattacatgg
    14941 cctgaaacat caattatttt taagtgccga tttaatatca ggaaatttaa agttaaaacc
    15001 cgcctgctga attttagtac tgcttaccgt cgcacttttt aaaatttcaa tactcatttc
    15061 tcccagcaac agtttcagaa taaaggaggg tacataaaat gggatgaaga atttctttct
    15121 ggatattgcc agttccagcg ttagttcttt attactgacg ggttttgggg ctactgcatt
    15181 ataaacacca ttcatatttt cattctcaat agcagccatg aacatattca caagatcatc
    15241 aatatggatc cagctgatca tttgctttcc attacctaaa atggctgcta ccccgaattg
    15301 taaaggtttg atgaattcct ttaacgctcc gccatcctta ctcaatacaa ttccaattct
    15361 caatttaact accctctttt tcaattgggt gaccggttct atactttgtt cccattgacg
    15421 acaggtatct cctaaaaaag cagtatcagc cggtgcatct tctaaaaaag gaattggatt
    15481 aggaataaca ggatcagcgc cataccatcc gatagcagaa gcagtaataa ccgcctttac
    15541 attgttctct atttcgctta atgcagtaac taacaaagcc ccgcttttaa cccggctgtc
    15601 cacaatttcc tgttttcttt tctttgtcca ccgtttatct gctacaccgg cacctgccag
    15661 gtgaatgata taatctgcct tggcaacggc atctttatcg attgtttgtg cctcaacatt
    15721 ccattgtacc cattgtattc catttttggg ttgcgggctt atggccatct gctttgtact
    15781 ccttgttagg ataataacat tatgtttttt ctccagcaag gcactgctaa gagccctgcc
    15841 caccaatccg gttcctcctg ttaataaaat agttgccata attgaaatat gaaaggtagt
    15901 tgttaaaata acataccggt caacatattg ttggttgata tactaaagaa acttttatct
    15961 tcaggccgca aataaaatac gttgtatgag tattgttcta tcaataaaaa atatagccaa
    16021 atcttacggg tctgtccgtg cattgaatgg agtgtcattt gatgtaccgc agggaagtgt
    16081 atttggcatc cttggaccga acggcagcgg caaaaccaca ttgctgggta tcgtaatgga
    16141 tgtgctgaaa gcaaataccg gagactttga ctggtttggc cagcctggct cccctgaaca
    16201 gcgaaaaaag atcggctccc tgctggaaac acccaatttt tacagctata tgtctgctat
    16261 agataattta aaaattacac agtccatcag tggccgcggt acttcagctg acatagatgc
    16321 agttttgaaa aaagtaaact tatacgacag gcgacggtat aggttcaatt cctacagtct
    16381 gggtatgaaa caaagactgg ctattggtgc agccttatta ggcaatccca aagtgctggt
    16441 gctggatgaa cctactaatg gacttgaccc ggttggtatt gcggagataa ggcaattgat
    16501 cattgaactg agagaccagg gacataccat catcatggcc agccacctgc tggatgaagt
    16561 ggaaaaagta tgcacccatg tggctatctt aaaatccggc aacctgctta ccaccggaaa
    16621 tgtggaagaa gtaatgatgg atgaagatat tgtggaatta agcgctgcag acttaactgc
    16681 gcttacagca ctgtttagaa aaagagaaca acagataatt tttgacgaga gggccaatac
    16741 cctccaggta atactgccaa aaggaactgc aaagatggaa gacatcaatc gtctctgttt
    16801 tgaaaatggt atagtactca atcaccttat actgaaaaag aaaagactgg aagcaagatt
    16861 ttttgaatta accaataact aatcctgaaa tttcaaatat gataaagctg atcaaaacag
    16921 aatggttgaa gatgaggaag tataccgcct tttggtggat aatgggcctt accgctctcg
    16981 cctacccggg catcaattat gccttttata tggtgtatga caacctgatg aaccagccaa
    17041 cgcaagccag taagatggca aaaatgctta ttggcaaccc attcactttt ccggaagtat
    17101 ggcatacggt cgcttatttt tcttcctggt ttgtatttat tccggctgta gtggtcatca
    17161 tgttcatcag taatgaatat tcattcaaaa ctcaccggca aaatatcata gatggatgga
    17221 gcaggaaaca atttattacc agtaaactga tggatgtaat gatcatatca ttactgatct
    17281 cagtattgta cattattgtt tccatggttt ccggatatgc cagcaaaagt gagggttccg
    17341 gggaaatgtg ggacaagtct tactttatcg gactatttac tctgcaaact tttgctcagc
    17401 tttccatcgc attcctgatc ggctttttag taaaaaaagc ttttatagca ctgggcattt
    17461 ttatcttcta ttttctgata cttgaaaata tcctcgtcgg tttattcaaa gtaaaagtga
    17521 atgatattgg tcgctattta ccatttgaga tttctgaccg gatgatacca gttccgggat
    17581 ttttgggaaa actggagtct gccgaaaatt accagaaagc atttgatgac attacacttc
    17641 atgtaattct cactttaatc cttagcgtta ttatctgggg tatttgttat tggataaaca
    17701 gtaaaaagga tttaaagtaa aatccttttt actccttgta tttatagtta tcgggacctt
    17761 tgtgcgccat aacttcttcg ctgactgtta cctccctgcc cggctgctgt attaaaattc
    17821 cgccgtggtt taaaagattg ctgttgcctt gtcgctggtg ccggtgctgc aatatgtgca
    17881 tttgctgtaa atggatgttc atgcaccacc ggtatcgttt tactaatcag tttctgaata
    17941 tcttttaact cagccctctc ttcggtacta cagaaagaaa tcgctgtacc aatatttcct
    18001 gccctgccgg tacgaccaat acggtgtaca taggtttcag gaatattcgg taattcaaag
    18061 ttgatcacat gggtcagttc atctatatca attcccctgg ctgcaatatc tgtggccact
    18121 aaaacacgga gtgtgccatt cttgaaatca gacaatgcac cctgccgggc attctgcgat
    18181 ttattgccat gtatggcagc ggccctgata ttatggcgtt gtaagatctt caccacttta
    18241 tcggcgccat gtttagtacg ggtaaataca agggctgttt taatttcttt atttccgagc
    18301 aggtgaagca gtaattcagg cttctccatt ttgcctacat gataaaggct ttgctctatt
    18361 ttatcaaccg ttgaggaagg cggcgtcact tccacttttg caggatgtgt caacaataca
    18421 gcagccaact tttgaatctc gggtggcatt gtagccgaaa agaaaagtgt ctgtcgcttt
    18481 gcgggcagtt ttgcgatcac tttttttacg tcgtgaataa aacccatatc aagcatacgg
    18541 tctgcttcat ccagtacaaa aatttgcaac ttatccaggt gaatataacc ctgagacatc
    18601 aagtcgagca gacggccggg tgtggccacc agtacatcca ctcccctgcg cagggcattg
    18661 gtttgatttt gctgagatac accgccaaaa ataaccgtgt ggcgtatgcc ggtaaatctt
    18721 ccataagcag caaaactttc atctatttgt atcgccagtt ccctggtggg agttaagatc
    18781 aatactttga taccagctgc cagtggtttt tgttcataca gaatctgcag gataggaatg
    18841 gcaaaggcgg ctgtttttcc ggtgcctgtc tgggcacaac ccaggaggtc ttttcgttgc
    18901 aacaccagcg gaatagcctg ttgctgaatg ggggttggga ttgtgtaacc ttctgtgctc
    18961 aaagccttta ggataggttc aatcaatgat aatttcgtaa aagccattat atggttttat
    19021 gtttaattcg cagatcatct gcggatggag ctggcaaaga cgttgtagtt tctgaatcag
    19081 cgaaaatttg ttctgtgtac caccggctta caacctgccg tgtcagttat gtggagtagg
    19141 atgcgaagat atggttttat taatttacat gtgtaaaaca gtaattagtg tcttgttagg
    19201 gttgttatta tacactgcat ttttaaatca ccttattttt ctcagcatac aaaaggatat
    19261 acttcttata aaaaaaattc tttgtctttt gcattcattt cctttattgg ctgcaattgc
    19321 ttaaaatgag gccagccagt atcttccacc cacagcagtt catagtattt cgccggaaca
    19381 agaatggtag atatggccac ttgcatcagt tctatctttt ctctttcggt aaattctttt
    19441 ggcggtattc ctgcttcccg cacaccgata aataataaga tatcctccag tgtaagcttt
    19501 tttcccaatc gttctgttaa tgcatattcc agttgaaacc aatgttgttg cagttcatta
    19561 tcgggaagca taatacaaag gtaactccag aacataaagg attatcgggt aattgatgaa
    19621 tagaattacc aggtgtataa aattgataaa ttgaatatat ctcaccggaa gttgatcaat
    19681 taacaattcg cttttagtga atataatgga agatgattgt agtatactaa ttgagaaaaa
    19741 tattaaaaag aaaaaaaacc gggccaaccc tgttatgata tattttatac aaaaaccctc
    19801 cggtggaaac cggagggaac aactaaaaaa cactagctaa atgctgagtg gcttcattgg
    19861 aacgaacaag ggtctcggac tgtattatta taatcagtcg ggtttctttc cgtctaagaa
    19921 tgtattaatg gtgctgatat gtccctggtt tttgtcgtca gatttgacct cgtagctgct
    19981 gtaaaagttt tcgacctggg tattgctgtt atacaattcc atattgcggc ggatatatgc
    20041 cggcacagtc tcaaactcat tattggggtc agcagcgtta acattgaatg acaaatttct
    20101 caacttgtgc aatcgttccg ccgccctacg tttttgctct tcggtttcgt cctgcagagc
    20161 agaatcctca acctcggtgg acaaaggtga ctgagcctga tgggccatcg gc
//
LOCUS       DAORTI010000059        20106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2287_length_20106_cov_8.681412, whole genome shotgun sequence.
ACCESSION   DAORTI010000059 DAORTI010000000
VERSION     DAORTI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20106
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_20106_cov_8.681412"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PLO70_09070"
     CDS             complement(<1..1202)
                     /locus_tag="PLO70_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HQZ74657.1"
                     /translation="MRKKQIELLAPVGSFDSLQAAIKGGADAIYFGVEQLNMRTRSAQ
                     SFYLSDIKEVSGICKRNGIKAYITLNTVMYEHDMQLLQMILKEAKKAKIDAVIAADFA
                     VIEYCSQHGIPLHISTQANVSNIESVKFFSRFADVIVLARELTLKQVKEIADEIKRKK
                     IKGPSGELVKIEIFVHGALCMAVSGKCYLSLHTQHASANRGACVQNCRRPYIVKDAET
                     GEELMVDNEYIMSPKDLCTIDILDQVMESGAEVLKIEGRTKGAEYVQVVTQCYREAIE
                     SVNDGTYSRGKVVEWKREMEKVYNRGFWEGYYLGRKLGEWTPHPGSAATEKKIYIGKG
                     TKYYPKILVGEFILESGSLKAGDTLMVIGPKCGMVKEIVTKLIINGISGKEAVKGDKI
                     TFPFATKIT"
     gene            complement(1325..2692)
                     /locus_tag="PLO70_09075"
     CDS             complement(1325..2692)
                     /locus_tag="PLO70_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQZ74658.1"
                     /translation="MKKFSLLFQFGAFFILFPTVIKAQNVGIGELLPQAKLEIRGVNV
                     LSNYNALLVKNISNDSLFRISNAGELIAGSKSKGYGQLQVINNGNQFSHLILKGTNDN
                     NSGSFFTRARFADETESRYWQLRSRVYGNGSGMMNNSRNSSFSIGSDSLPDMITVDGT
                     GKIGIAESFIPLAYLDIAVNFQAEGVPHLMLRGTGVNSQSIQAFANISGSDYWYLDSR
                     HYAFDPASSSFNIGYNGTAKLSLRPSGNFGIGTGDPQHTLHIKTNSSHAGLVHMLLEE
                     SEEDFSRIRFQNTIANRYWEIGSFSQNLNNSSEMNFYYNNSGVILSLKGDGNATLMGN
                     LTQLSDARLKKNITLIRDALPALMNINGYRYNWKDPEKDKTLQVGLLAQEIEKVMPEL
                     VIENNDGIKSVNYAGMIPVLIEAFKEQQLEIKALITENKRQSKMIKQLQNEILDIDLK
                     GKVKH"
     gene            complement(2696..4171)
                     /locus_tag="PLO70_09080"
     CDS             complement(2696..4171)
                     /locus_tag="PLO70_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQZ74659.1"
                     /translation="MRKILFLLLSFLLFSSVIPAQSVAVNTDGSLPDVSSLLDIKSIS
                     KGVLIPRMNRAQRDAISTPATSLVIYQTDAEAGFYYNGGTPGFPQWKLMGDNVLPASA
                     LVLSETKPNSMLQNAGFSLNADIQLTLTNATIPANTWSEINMNNSIPDDEASYISAKA
                     GDYIVMMKGGGVIFEIFTYRISTNTWTRAFVGNNLDVNIANDMQSGYALLTDGTYVYS
                     IPPHTRFPINCIPPCPYYAGIKFNPANNEVLYFPVNLNHSGRSAYTLIMAGNKILIWG
                     GWDNNSNMYFNDGLLIDLLSGTWSAVSGSPSLSPRRSASGVWTGAEVIIFGGENNGTY
                     YTDGARLNLSTLTWVPIASNPSIVFPIHYSHWNGSEMFLFGNAGFKFNSSLNSWSSVN
                     AGLNAIHFGENFAWTGSEYWRYNPLTQLITKFNPTTNTVSSAHPGGPSWGGGGGSVNN
                     WCGDKLFVMNYYHPAITSRFFYPNQTNGVAANRTFYLYKKN"
     gene            complement(4629..6161)
                     /locus_tag="PLO70_09085"
     CDS             complement(4629..6161)
                     /locus_tag="PLO70_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HQZ74660.1"
                     /translation="MIKAANKVIPLVYSCSGCSSSAQMTNYLAIQLDRKGLAEMSCIA
                     GVGGNVKSILSTAQSKREIIALDGCPLACTRACLLNHAIKPDIHIELTSFGVKKKYHE
                     DFDLHKAREIQQVLEHTILKGREKLKGDSPAVATITEIRIEEVKTTKQLRDFIDFPLR
                     LYKDNAYYVPQLTKDIEATFNHQKNPAFDFCQARYWLAYKNDELAGRIAGIINHSFIA
                     TWKKPYLRFGWFDFVNDELVSKALLAEVEQWARQKEMKAVHGPLGFTNFEFAGMLTEG
                     FNERGTFATLYNYPYYPFLLEKAGYKKETSWVEYKIKISDTIPEKILQVASIVEKRYK
                     LKVIKPTKLKQIRPFAEKIFELINKKYAGLFGMVPMSDKQVQYNIRKYVTFIKPDFVS
                     LVLDKDGELTAFGIAMPSLSKALQKVNGELYPFGFLHLLKAFKRNKFADLCLVAVRKD
                     FQGKGVNAILMREINTAFIRNGIEYAESNPELADNHKVQSQWDYYEAVQHKRRHCFIK
                     HL"
     gene            complement(6289..8577)
                     /locus_tag="PLO70_09090"
     CDS             complement(6289..8577)
                     /locus_tag="PLO70_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQZ74661.1"
                     /translation="MAKKNFINYLLNPKNWWLPLLIIFTVSLTGVTMIGIHTYTEAPP
                     IPDYVSSNNEKVFSKDDVLNGQAVFQRYALMEYGSMFGDGANRGPDYTAEALHYVSHY
                     MNIFYQNQLVISENREWQQKGMAEQVRSEIKNNQYEKNTNKVKLTDAQVFAASELVKY
                     YQQKFTTAESPGAFKPVGYLTDTTEIRSLTAFFFWGAWVCGVQRPGEIYSYTHNWPFD
                     PDAGNTPSSAIIQWSILGSLGLIFGLGLVLYYYGKLEKLDDNAYTSNAQPFMTRDEIH
                     KFKPSAVQRATYKFFFTAIILFAVQVLSGILTVHDFVGFVNFFGFNISEALPITVIRS
                     WHVQLSILWISACWIGGSFFMMSLVSPNQNSAQVKLINIIFWLTVLLVAGSIAGILLG
                     PKGVFGKNWYWFGHQGWEYVEPGKIWQIILGMVFIIWAITLYRGIKPVMKLKHPWALP
                     NWLVYATFSIIILLISGFIATPKTNFVIADFWRWMVVHMWAEAFFEVFTTVLIGYFMV
                     LMGLVSKQAATRVIYLATLLFLGSGLLGISHNFYWNAKPVFTMALGSVFSTLQVVPLV
                     LLTLEAWRFSKLPKVLENGYNNGNPARRFGFSEVFLFLVAVNFWNFFGAGVLGFIINL
                     PIANYYEHGTYLTVNHGHAALMGVYGNLALATVLFCSQLLIKPDCWRPRIIKNAFWSI
                     NVGLLLMVFLDLFPAGIWQFKSVTDNGLWFARSHEFIESTGFQTLTWLRIIGGSLFTL
                     GGVIPLVWFITTRSMRLKEKIT"
     gene            9028..10422
                     /locus_tag="PLO70_09095"
     CDS             9028..10422
                     /locus_tag="PLO70_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQZ74662.1"
                     /translation="MAPSNNRQENVPVLFMIVAMILLATGMIWGLTGGLQYVIPGFLK
                     QYLSFEKVRPLHVSSVVFWIILGAMGAVFTYLQQHTGKKIYSQTFLKIQLVIFIFSIT
                     GILISYSLGIFGGREYWEYPPVFALPIAIGWILFLINFIKSVGSLKKQPVYVWMWLTG
                     VLFFLFTFLESYLWIFPYFRNNIVNDMTVQWKSYGSMVGSWNMLIYGSSIFLMDKISN
                     TKSYSHSGIAFILYFTGLFNLMFNWGHHIYTLPTHTYIKHISYAVSMTELFILGRIIY
                     QWKSTLSLAKKNFHLIAYRFLAAADVWIFLTLLLAIFMSIPGINVYTHGTHITVAHTM
                     GATIGINSFLLLAIAFDIFSESCYSFESYKKIVNRGYWITNISLFVFWVSLIGAGIIK
                     ARWQLSTSQVPFSDMMLQLRPYLILFVIAGTTMTTGFYFVIFPLLKNQLLCYFGKATK
                     ERKQQDIIHLPVSE"
     gene            10457..10876
                     /locus_tag="PLO70_09100"
     CDS             10457..10876
                     /locus_tag="PLO70_09100"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQZ74663.1"
                     /translation="MVTKIFNFEMAHAISDYPGSCNNIHGHSYELHVTVSTINQEKCY
                     LAPPGFILDFKELKKLVNTHVIQALDHKLILSKNYLGKNSSINPLENLVPWESEPTAE
                     NLLLYIQSVLSENLPVNIKLAKLKLYETKGSYAEWIS"
     gene            10926..11411
                     /locus_tag="PLO70_09105"
     CDS             10926..11411
                     /locus_tag="PLO70_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQZ74664.1"
                     /translation="MFSKATEYALRATIHIAKNSSENNKLGLAVIAAAIDSPQSFTAK
                     ILQLLTRNNKVISSVRGPNGGFYITKKARKLPVRAILEAMHEDKVLEKCVLGLAECSE
                     SQPCPMHSQYKQIKHQLINLFENKTIEYLAEEMTEGITFINNKSKKIKVKIEKPGAKK
                     S"
     gene            complement(11443..12432)
                     /locus_tag="PLO70_09110"
     CDS             complement(11443..12432)
                     /locus_tag="PLO70_09110"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HQZ74665.1"
                     /translation="MKLHSTYMGLKLNSPILVSACTLSEQTDNIVKMEDNGAGAVVLF
                     SLFEEQIRKEEARFKGVMSETTYAFPEALDYFPDLDDFNVGTDEYLENIRKAKERVNI
                     PIIGSLNGITTEGWIDYSKLMEQAGADGIEVNIFFIPGDIAMSSSEVEHRYLNIIETI
                     KQSVKIPVAVKLNPYFSAMGNMSLRMKNAGADALVLFNRFYQPDFDINELVIKTDLHY
                     SEPNEIRLPLLWIALLYGKVKLSLAATTGVQSAIEVIKYILAGADVVMTASSLYKNGI
                     PYLKTMNKELQDWMYMMGFESIDSFKGSMSQQHISDPTAYERANYIKILEGVK"
     gene            complement(12443..16018)
                     /gene="nifJ"
                     /locus_tag="PLO70_09115"
     CDS             complement(12443..16018)
                     /gene="nifJ"
                     /locus_tag="PLO70_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQZ74666.1"
                     /translation="MSSNKKIIATIDGNEAAAYVAYRVNEVCAIYPITPSSTMAELAD
                     EWASKGIKNIWGTVPDVMEMQSEAGAAGTVHGALQTGSLTTTFTASQGLMLMLPNMYK
                     IAGELTPAVFHVAARSLAAQGLSIFGDHQDVMAARTTGFAMLGSASVQEAHDFALIAQ
                     VATLKSRIPIVHFFDGFRTSHEVNKLEMLSDEQIKSMIPDELVIAHRNRGLNPEHPFI
                     RGTAQNPDVYFQGRETVNKFYNAMPSIVEEVMNDFAKMTGRKYELFEYSGAPDAEAIT
                     IIMGSGSETVEETVKALNKSGRKTGVIVVRLYRPFSLDHLLKVLPASVKSIAVLDRTK
                     ESGAAGEPMYQDVLATLVEALQQGKLKKLPNIVGGRYGLSSKEFTPAMVKGVYDELEK
                     KHPKNGFTVGINDDVTFTSLEYDPAYKLDETEWRQGLFFGLGADGTVGANKNTIKIIG
                     ENTDLYAQGYFVYDSKKSGARTVSHLRFGPNPIRAPYLISKADFIACHQFNFTEKVEM
                     LEFASDGATFLLNSPYSKDEVWDKLTGQVQKSIIDKKLKLYVIDATTVARDTGMSGRI
                     NTIMQTCYFALSGVLPREDAIKQIKKAIEKTYFKKGQKVIDQNFKAVDATLANLYEVD
                     YPKKVSAADNDLQTVSDKAPDFVKEVTAVMMAGHGDDLPVSKMTVDGTYPSGTTQWEK
                     RNVSDLVPIWEPNICIQCGNCAYVCPHSVIRAKFYHKDHLKKAPADFKSAPINARGFP
                     ETQYSLQVYLEDCTGCNLCVEVCPATDPANRSLKAINMAEKEPVLKAEQKNIEFFENI
                     PWNDRSKIDFSTVHGAQFLEPLFEFSGACAGCGETPYLKLLSQLFGDRLLVANATGCS
                     SIYGGNLPTTPWAVNKEGRGPAWSNSLFEDNAEFGLGMRITADKQVGVAKQYLLELKD
                     VLGESFVNEIINAKQIHESELAEQQKRVQLLKEKLKTMTSPAAIHLLSVADQLVRRSV
                     WLVGGDGWAYDIGYGGLDHALASGRNVKILVLDTEVYSNTGGQSSKSTPTAATAKFAA
                     GGKSGGKKDLAMQAISYGNVYVARVAFGANPQQTLLAMREAEAYDGPALILAYSHCIA
                     HGYDLKNGLDQQHNAVASGHWPLLRYNPTLRMKNQNPFVLDSPRPTIGLKEYAYNELR
                     YKVLTITNPEAADTLMKHAQELVNLKWKTYEELATKKASDFVPIA"
     gene            complement(16008..17654)
                     /locus_tag="PLO70_09120"
     CDS             complement(16008..17654)
                     /locus_tag="PLO70_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQZ74667.1"
                     /translation="MAATDITKAVDLSIHAEGTGPKRLRRPVYVDFMPPCNSACPAGE
                     NIQAWMAHAQSGNYFEAFQTLVEDNPFPAVMGRVCVKPCETGCNRNHIDDTVNIHAVE
                     RYIGDEAIKQKWPVQFVVNSTEKKVLVVGAGPGGLSAAYHLARMGHTVEVYDAGGHPG
                     GLLWTGVPDYRLPKEILDAEVDRIVKMGVKIRLNYKVQDVLTEKQAGNFDAVYLSIGA
                     QLIRKEDFQHDDSVYITDAFSFFNEVKSNTSPYVRKKVVVYGGGKLALYLARMIKRFG
                     SEVSVYFPGDNKMMPAYDYETEDALAEGVDVQLFRSISKIEKYKMTLEKMKVEKGKAV
                     GTGEFETTDADVLIIANRQESDSGFLRSVAGIVVNEDGTVAINAERMTGYEGIFAGGD
                     MLPGENRSSTIAIGHGKKAAKYINGYLMQEPYQKPEKHPTASYRKLHMWYKTDAPQKE
                     QDKLAPAVAVKSFEEVITGLSEPEARFEAQRCLSCGNCFECDGCFGACPEDAIIKLGK
                     GNRYKFNYNACTGCAVCYEQCPCHAIEMIPEPVNSTTDVK"
     gene            complement(17885..18409)
                     /locus_tag="PLO70_09125"
     CDS             complement(17885..18409)
                     /locus_tag="PLO70_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74668.1"
                     /translation="MKRCLIFSLAIITSSVLFAQPGNVGKVPGRTQFYAELGGPGILF
                     SANIDTRFAKSSLGWGGRIGLGFVTADEDLGNYNYELRSVVTVPAQVNYIFGKGDSPH
                     TFEVGAGITYVGRKLDIFNFYDDKTSNVFGTASFMYRRQPIKGGFTWRIGFTPLIANG
                     YIQPSGGVSVGYSF"
     gene            complement(18497..19963)
                     /locus_tag="PLO70_09130"
     CDS             complement(18497..19963)
                     /locus_tag="PLO70_09130"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQZ74669.1"
                     /translation="MANDTNKFQAIISHCKEYGFIFPSSEIYDGLQAVYDYGQMGSEL
                     KKNIKDYWWKSMTQLQDNIVGIDAAIFMHPSTWKASGHVDNFNDPMIDNKDSKKRYRV
                     DHLIEGYAEMLKAENQMQKADDLLASMDALLAKDDFEGLKKLIEDNKISCAISGTCNW
                     TDIRQFNLMFSTEFGSTVSSDDEDNKVYLRPETAQGIFVNFLNVQKTGRMKIPFGIAQ
                     IGKAFRNEIVARQFIFRMREFEQMEMQFFIRPGTQKEWYEFWKTERLKWHQSLGIPAE
                     KYRYHDHVKLAHYADAACDIEFEFPIGFKELEGIHSRTDFDLKQHQEYSKKKQQYFDN
                     DIDPATGKPFGNYIPYVIETSIGLDRMFLAVISQAYEEQDLSTAEKQDSRVVLKFPPK
                     LAPIKLAVLPLTKKDGLPEIAALIMKDCKPHFRCFYEEKDSIGKRYRRMDALGTPFCV
                     TVDHQSKEDNTVTIRHRDSMEQERVPVDKIKDIVLASL"
BASE COUNT         5923 a         4432 c         3542 g         6209 t
ORIGIN      
        1 gttatcttgg tggcaaaagg gaaagtgatc ttatcacctt tcacagcttc ttttccactg
       61 ataccattta tgattaattt cgttactatt tcttttacca ttccgcactt cgggccgata
      121 accatcaacg tatctcctgc tttcaggctc cccgactcca gaatgaactc acccaccaaa
      181 attttaggat aatatttagt tcccttgcct atataaattt tcttttctgt tgcagcggaa
      241 ccaggatgcg gtgtccattc acctaatttc cggcccaggt aatatccttc ccagaaacca
      301 cggttgtaca ctttttccat ttctcttttc cattcaacta cttttcccct ggagtaagtt
      361 ccgtcattca ccgactctat tgcttcccgg taacattgtg taactacctg cacatattcc
      421 gctccttttg ttcttccctc tatcttcaac acctctgcgc cactctccat cacctggtcc
      481 aggatatcaa ttgtacataa atccttggga gacatgatgt attcattatc caccatgagt
      541 tcttcgccgg tttcagcgtc ctttacaata taaggcctgc ggcagttctg cacacaagcc
      601 ccccggttgg cagaagcatg ttgagtatgc aggctcaaat aacatttacc tgatactgcc
      661 atacataagg cgccgtgcac aaaaatttca atcttaacta attccccgga aggacctttt
      721 atcttctttc tttttatctc gtcagcgatc tcctttacct gctttaatgt aagctctctt
      781 gccagtacaa taacatcggc aaagcgggaa aagaatttca cggattcaat attgcttaca
      841 ttggcctgcg tagagatatg caacggaata ccatgctgac tgcaatattc aatcacggca
      901 aaatcagccg ctatcactgc gtcgatcttt gctttttttg cttctttaag gatcatctgc
      961 agtagttgca tatcatgctc atacatcaca gtgttcaggg tgatataggc tttaatacca
     1021 tttcttttgc aaataccact gacttcttta atatcggaaa gataaaaaga ctgggcagaa
     1081 cgggtacgca tatttaattg ctctactccg aagtatatag catctgcacc cccttttata
     1141 gcggcctgca aactgtcgaa tgaaccgaca ggtgccaaaa gttcaatttg cttcttcctc
     1201 attattacca aattttgctc tctctgaaag acataaacca ctattgtact aaaaagcttt
     1261 ttatattatt agataagtct ttaatgctgc ataaaagata tatccagata ttatacaaaa
     1321 ttaatcaatg ttttactttc cctttcaaat caatatccaa gatttcattt tgtaattgtt
     1381 ttatcatctt actctgcctt ttattttcgg ttatcagagc ttttatttcc agctgttgtt
     1441 ctttaaaagc ttcaatcaaa acaggaatca tacctgcgta gttgactgat tttattccat
     1501 cattattctc tattacaagt tcgggcatca ctttctcaat ttcctgagcc aataatccta
     1561 cctgaagtgt tttatccttt tcaggatctt tccagttata ccggtatcca tttatattca
     1621 tcagtgcagg caatgcgtcc ctgattaagg tgatattttt cttcagcctg gcgtctgaga
     1681 gttgtgttaa gtttcccatt aaagtggcat taccgtcacc tttcagggaa agaataactc
     1741 cggaattatt gtaataaaaa ttcatttctg aactattatt caaattttgg gaaaaagaac
     1801 caatctccca gtagcggttt gcaatggtgt tttgaaaacg aatcctggaa aaatcttctt
     1861 cagactcttc gagtaacata tgaactaaac cggcgtgact actattggtc ttaatatgca
     1921 gggtatgctg cggatcgccc gtaccaatac caaaattacc actgggccgc aaactcagct
     1981 ttgctgtccc attatacccg atattaaacg aagaacttgc aggatcaaac gcatagtgcc
     2041 tgctatccag ataccagtaa tcactcccac ttatatttgc aaaggcttga atgctttgac
     2101 tgttaacacc tgttcctctt aacattaaat gaggaactcc ttcagcttga aaattaactg
     2161 ctatatccag gtaagccaat ggaataaagg attcagctat cccaattttc ccggtaccat
     2221 caacagttat catatccgga agactatcag accctattga aaaactacta tttctgctat
     2281 tattcatcat accggaacca ttgccataaa cacggcttct cagctgccaa tacctgcttt
     2341 cagtttcatc ggcaaatcta gcacgggtaa aaaaacttcc gctgttatta tcattcgttc
     2401 cttttaaaat aagatgacta aactggtttc cgttattgat aacctgcaac tggccatacc
     2461 ccttagattt gcttccagca atcagttcac ctgcattaga aattctaaac aacgagtcat
     2521 tacttatatt tttaaccagc aaggcgttat aatttgataa tacattgacc cctctaattt
     2581 ctaattttgc ctgcggcaac aattctccaa tgcccacatt ttgcgctttt attactgttg
     2641 ggaataggat aaaaaaagca ccgaattgaa ataataaact aaattttttc atgtcttagt
     2701 tttttttata taaatagaaa gtacggttcg ccgctacacc gtttgtctgg ttgggataaa
     2761 aaaagcggga tgtaatagcc ggatgataat aattcataac aaatagttta tccccacacc
     2821 aattatttac ggatcctccc cctccacccc atgaaggtcc acccggatga gcagatgaaa
     2881 ctgtgtttgt agttggatta aatttcgtaa taagctgtgt aagagggtta tagcgccaat
     2941 attccgaacc agtccaagca aaattttcac cgaaatgaat tgcattaagt cctgcattca
     3001 ctgaagacca actattaaga gatgaattaa acttaaaacc tgcgttacca aataaaaaca
     3061 tttctgatcc gttccagtgc gaatagtgta ttggaaaaac aattgaagga ttggaggcaa
     3121 tcgggaccca ggtaagtgtt gacaggttta accgggctcc atcagtataa taagtaccgt
     3181 tattttcgcc accgaaaata attacctctg cacctgtcca cactcctgag gcgcttctgc
     3241 gtggcgatag tgacggactg ccagaaacag cagaccaggt accagataaa agatcaatta
     3301 ataaaccatc gttaaaatac atatttgagt tattatccca gccaccccaa attaaaatct
     3361 tatttcctgc cataattaga gtgtaagcac ttcttccgga atgattcagg ttaaccggga
     3421 aataaagtac ctcattattg gccggattaa attttatccc tgcataataa ggacaaggtg
     3481 gtatacagtt aatcggaaat cttgtatgcg ggggaatgct gtatacataa gtaccatccg
     3541 taagtaaagc atacccagac tgcatatcat ttgcaatatt tacatccaga ttatttccta
     3601 cgaaagctct tgtccacgta ttagtagaaa ttctgtatgt aaatatttcg aagattacac
     3661 cccccccttt catcattaca atatagtctc cggcttttgc tgatatatag cttgcctcgt
     3721 cgtcaggaat actgttattc atgttaattt ccgaccatgt attagcaggt attgtagcat
     3781 tggttaatgt cagttgaata tcggcattca ggctaaaacc agcgttctgt agcatactat
     3841 tgggcttggt ctcactcagt actaaagcag aagcaggaag tacattatct cccattaatt
     3901 tccactgcgg aaatccgggg gtgcccccat tgtaataaaa tcccgcttca gcgtctgtct
     3961 ggtaaataac aagactggtt gccggagtag agatagcatc tctttgtgca cggttcatcc
     4021 ttggaattaa aactccctta ctaatacttt ttatatcaag taaagaactc acatctggca
     4081 aagaaccatc ggtattaaca gcaaccgatt gcgcaggtat tacgctgctg aatagtaaga
     4141 agcttagcag aagaaataat atttttcgca taaccagtaa ttattaggtt aaaaattaat
     4201 taacacttta taaatgaata aactatccat ttatttaaaa atgctgctac ttacctggag
     4261 cagcttattt ttttaatttt taaatttgac ataaatgaaa ttaattactc ttagaaaagc
     4321 agcaacagca aagggtattc atcctgcaaa agcctgagca aggattataa tcgaattggt
     4381 aagtagtagt tgtaagccag taacagttta aaatattgcc ggtgctatga acacgaaaat
     4441 agaagaatgt ttaatacccg ctgttacaaa ttatgtttac aaattaatgc cagtttgcaa
     4501 gaagtctcat tcctgtattc aaatccttac aacctttgta taaatgtaca gattttttta
     4561 gattcagtag tacggtttca gatacatccg tttgagaaac gcaaaacaat actttcgtgt
     4621 cttctatttt ataaatgttt aataaagcaa tgtcttcgct tgtgttgtac agcttcataa
     4681 taatcccatt gtgattgtac tttgtgattg tctgccagtt cggggtttga ctcagcatac
     4741 tcaataccat tacgaataaa agcagtattt atttctctca taagaatcgc attcacaccc
     4801 tttccctgaa aatcttttcg tactgctacc agacacaaat ctgcaaattt atttctttta
     4861 aatgccttta gcaggtgtaa aaacccgaaa ggatataatt ccccgtttac cttctgtaaa
     4921 gcttttgata aagaaggcat agcgatacca aatgcggtaa gttcaccatc tttatccagt
     4981 actaaagaaa caaagtctgg cttaataaaa gttacgtact tgcggatatt atactgaacc
     5041 tgcttatcag acattggtac cattccaaat aaacctgcat attttttatt aattagttca
     5101 aatattttct ccgcaaaagg ccgaatttgt tttagtttgg ttggtttgat cactttaagt
     5161 ttatatcgct tttcaacgat tgaagcgact tgtaatattt tttcgggtat agtatctgaa
     5221 atctttattt tatactcaac ccagctggtt tcttttttgt agcctgcctt ctccaataaa
     5281 aaagggtaat atggatagtt atatagggtg gcaaaggttc ctctttcatt aaaaccctct
     5341 gtaagcatac cagcaaattc aaaattggta aatcccagtg gaccatgtac cgccttcatt
     5401 tctttttgcc tggcccattg ttctacttca gccagcagcg ctttggaaac taattcatca
     5461 ttcacgaaat cgaaccagcc aaagcgcaga taaggtttct tccatgtcgc gataaaagag
     5521 tggttgatga ttccggcaat gcgaccagcc agttcgtcgt ttttatacgc cagccagtat
     5581 cttgcttgac aaaaatcaaa tgccggattt ttctgatggt taaatgtggc ttcaatatct
     5641 tttgttaatt gaggcacata atatgcattg tccttataaa gacgcagcgg aaaatcaata
     5701 aaatcccgaa gttgctttgt tgttttaact tcctcaatcc gaatctctgt gattgtggca
     5761 acagccggag agtccccttt tagcttttcc cttcctttta gaattgtatg ctccagtacc
     5821 tgctgaattt ccctagcctt atgcaaatca aagtcttcat gatacttctt tttcacacca
     5881 aaagaagtta gctcaatatg tatgtctggt ttaatagcat gattcagcaa acaggctctg
     5941 gtacaagcaa gcgggcaacc atctaatgca attatttccc gctttgattg tgcagtactt
     6001 aaaatgcttt ttacattacc tcccactcca gcaatgcaac tcatttcagc aagacccttt
     6061 ctatcaagtt gaatggcaag atagttcgtc atctgagccg aactgctgca tcctgaacag
     6121 gaataaacta aggggataac tttatttgct gcttttatca tttaaaacat tttgccttac
     6181 acgattcata atcaacacaa agaatagctc caggcgccaa actgagtgaa ttttaatttt
     6241 aaaaaggaaa tattgcaatt tattcatctg tacttttaac tttcgattct aagttatttt
     6301 ttctttcagc ctcatactcc ttgtcgtaat aaaccaaact aatgggataa caccacccaa
     6361 tgtaaataac gaacctccta tgatacggag ccaggtaagt gtctgaaatc ctgtactttc
     6421 aatgaattca tgactacgtg caaaccagag accattatcg gtaacggact tgaattgcca
     6481 gatacccgca ggaaataaat ctaaaaaaac catcagtaac aaccctacat tgattgacca
     6541 gaacgcattt ttgatgatac ggggccgcca gcagtccggt ttaatgagta attgactgca
     6601 gaataatact gttgctaatg ctaaattacc atatactccc atcagagctg catgcccatg
     6661 attgacagtc aaatatgtgc catgttcata gtaattggca ataggaagat tgataataaa
     6721 acctaataca cctgcgccaa aaaaattcca gaaatttaca gctacgagaa ataagaaaac
     6781 ttcagaaaat ccaaaacgcc gggctggatt tccattatta tatccatttt caagtacttt
     6841 tggcaatttg ctgaatcgcc aggcttctag ggttaacaat accaagggta ctacctgtag
     6901 tgtagaaaaa acggagccca gtgccatagt aaataccggt ttggcattcc aatagaagtt
     6961 atgggagata cccagcaatc ccgaacctag aaataataat gtggctaaat aaatcactct
     7021 tgtggcagcc tgtttactta ccagccccat cagtaccata aagtagccaa tcaatactgt
     7081 agtgaaaact tcaaaaaatg cttctgccca catatggacc accatccagc gccagaaatc
     7141 agcaattaca aaattggtct ttggtgtagc aataaatcca gatatcagca aaattataat
     7201 actgaaggtg gcatatacga gccaattagg caacgcccaa gggtgtttca atttcataac
     7261 aggtttaata cccctgtata atgtaatagc ccagataata aataccattc ctaaaatgat
     7321 ctgccagatt tttcctggtt cgacatattc ccacccctgg tgtccgaacc aataccagtt
     7381 ttttccaaac acacccttgg gtccaagcag aattcctgct attgaaccgg caacaagcaa
     7441 aacagtaagc caaaaaataa tattaatgag ttttacctgt gccgaatttt gattgggtga
     7501 caccaacgac atcataaaaa acgaaccccc gatccagcag gcagaaatcc acagaataga
     7561 taattgtaca tgccagctac gtataacagt gataggtaac gcttcagaaa tattaaatcc
     7621 aaagaaatta acaaatccta caaagtcgtg aacagttaaa attccagaca gaacctgaac
     7681 agcaaataaa atgatagcag taaaaaaaaa cttataagta gcacgttgca ctgcactggg
     7741 tttaaattta tgaatttcgt ccctggtcat aaacggctgt gcgttgctgg tgtaagcgtt
     7801 atcatctaat ttttctagct ttccgtaata gtataatacc aaacccagcc caaatatcaa
     7861 tcccaatgaa ccaagaatac tccattgaat gatagctgaa cttggcgtat ttcccgcgtc
     7921 ggggtcaaaa ggccagttat gtgtataact gtagatttct ccgggtcttt gcacaccaca
     7981 tacccaagct ccccagaaga aaaaagcagt aagtgatctg atttctgtcg tatctgttag
     8041 gtaaccaacc ggtttaaatg cccctggcga ttcagcagta gtaaattttt gttggtaata
     8101 ttttaccagc tccgatgcag caaaaacctg tgcatctgtc agcttaactt tattagtatt
     8161 tttttcgtac tggttgttct ttatttcgga tcttacctgt tcagccattc ctttctgctg
     8221 ccattcccgg ttttcactga tcactaattg gttttgataa aatatattca tgtaatgcga
     8281 aacatagtgc agtgcttctg ccgtatagtc tggtccccgg ttagccccat caccaaacat
     8341 actgccatat tccatcaaag catatctttg aaaaactgcc tgcccattca gcacatcgtc
     8401 ttttgaaaag actttttcat tatttgaaga aacataatca ggaattgggg gagcttcggt
     8461 gtaagtatga attccaatca tggttactcc tgttaaactt actgtgaaaa taatcaacag
     8521 cggcaaccac cagttttttg gattcagtaa ataattaata aaattttttt ttgccatttc
     8581 tctgaattat gaggcaaata taataattaa ggatattttt gtccttaatt attatagaga
     8641 gaagtatgct ttcggaaaag tataagcaaa taatagcggt aaccctgata ataatctttt
     8701 tgttttattc gctttacctg tacctgtcct taccggtaaa agaagggccc gttacaaata
     8761 tggtacttaa gggtaaatca atctggcaac aaagtaattg catagcctgt caccaggttt
     8821 atggccttgg gggatacctg ggtccagatc ttacaaatac ctatacactc aggggcccgg
     8881 catatattac ggcttttttg cagagcggaa cagcgataat gcccaaattt catttgaaaa
     8941 agattgaaat agaagcctta ctagcttatt tgcaaaattt agatgcctca ggaaaggcag
     9001 accctagaac ttttacaata aacacgaatg gcaccatcga acaatagaca agaaaatgta
     9061 ccagtacttt ttatgattgt agctatgata ttattagcta caggaatgat atggggctta
     9121 accggggggc tccaatacgt cattccggga tttctaaagc aatacctctc gtttgagaag
     9181 gttagaccgc tacatgtttc ttcagtagta ttctggatta tactaggagc catgggtgcc
     9241 gtatttacct atttacaaca gcacaccgga aaaaaaatct actcgcaaac ttttttaaaa
     9301 attcaactag taatttttat cttttccatt actggaattt taatatccta cagtttgggc
     9361 atatttgggg gcagagaata ttgggagtac ccacctgtgt ttgccttacc aatagccatc
     9421 ggttggattc ttttcctcat caattttata aaatcggtgg gttcgttaaa aaaacaaccc
     9481 gtttatgtat ggatgtggct taccggtgtc cttttcttcc tgtttacttt cctggaatca
     9541 tacttatgga tattcccgta cttcagaaat aatatagtga atgatatgac cgtacagtgg
     9601 aaatcctatg gctctatggt tggttcctgg aatatgctca tttatggtag cagtatcttt
     9661 ttaatggata aaataagtaa taccaaaagc tatagtcatt caggtattgc ctttatactt
     9721 tattttaccg ggttattcaa cctgatgttc aactgggggc atcatatata cacgctgcca
     9781 actcatactt atatcaaaca catcagttac gccgtcagca tgactgaact tttcatactt
     9841 ggcagaatta tttatcaatg gaaatccaca ctaagcttgg caaaaaagaa ttttcattta
     9901 attgcatacc gctttctggc agccgcagat gtgtggatat tcttaacatt attactagca
     9961 atatttatgt caattccagg catcaatgtt tacacacatg gcacacacat aacagttgca
    10021 catacaatgg gtgccacaat cggcatcaat agttttttac ttctggcgat agcttttgac
    10081 atcttttctg aaagctgtta tagttttgaa tcctataaga aaattgtcaa caggggatat
    10141 tggattacaa atatttcttt attcgtattc tgggttagcc ttataggagc tggaataatt
    10201 aaggccagat ggcaactaag tacttcacag gtaccatttt ccgatatgat gctacaacta
    10261 aggccttacc ttattttatt cgttattgca ggcactacaa tgacaacagg tttctatttt
    10321 gttatctttc ctttattaaa aaatcaatta ctttgttatt ttggaaaagc tacaaaagag
    10381 agaaaacaac aggatattat ccatctgcca gtttcagaat aaatatatta cctgtttcta
    10441 aaattctatt atgttaatgg ttacaaaaat atttaatttc gaaatggcgc atgctatttc
    10501 tgattatcct ggctcttgta ataatattca tggacattcc tatgaattgc atgtaactgt
    10561 atctaccatt aatcaggaaa agtgctattt agccccgcct ggatttattt tagatttcaa
    10621 agaattaaaa aaactggtaa atacacatgt tattcaggct ctggatcata aacttatctt
    10681 gtccaaaaat tatttgggta aaaattccag tattaatcca ctggaaaacc tggttccctg
    10741 ggaatcagaa ccaactgccg aaaatttact actgtatatt caatcagttc tcagcgaaaa
    10801 tctgccagta aatataaagc tcgccaaact taagctttat gaaacaaagg gttcttatgc
    10861 agaatggatt agttaatata ttttaaatac ctgatataat ctttaatttc ttagtcttta
    10921 accaaatgtt ttctaaagcc acggaatacg ccttaagggc tacaatacat atagccaaaa
    10981 acagtagcga aaataataag cttggactgg cagtaattgc agcagcaatt gattcccctc
    11041 agtcatttac ggcaaaaatt cttcagttac ttacaaggaa taataaagtc atcagttctg
    11101 ttcgtggccc taacggaggt ttttatatta ccaaaaaagc caggaaactt cctgtaaggg
    11161 caatactaga ggcgatgcat gaagataaag tgctggaaaa atgtgtattg ggcctagctg
    11221 aatgttcaga gtcacaacct tgcccgatgc attctcagta taagcaaatc aaacatcagc
    11281 ttataaacct ttttgaaaat aaaacgatag aatacctggc agaagaaatg actgaaggca
    11341 ttacttttat aaataataaa agcaagaaaa tcaaagttaa aatcgaaaaa ccaggagcaa
    11401 aaaaaagtta aattcaattg ttgttttgaa gacagcacat tactatttca ccccttccag
    11461 tatcttaatg taatttgccc tttcataagc tgtcggatct gaaatatgct gctggctcat
    11521 actgccttta aatgagtcaa tactttcaaa acccatcata tacatccagt cctgcaattc
    11581 cttattcata gttttcaaat aagggatccc atttttatac agggaagaag cagtcattac
    11641 cacatcggca cctgccagaa tatatttaat aacttcaatt gcactttgca cacctgtagt
    11701 cgctgccagc gatagtttta cttttccata caataaggca atccataata aaggcaatct
    11761 tatttcattc ggctcactgt aatgcaggtc tgttttaatc accagttcat taatatcaaa
    11821 atcaggctga tagaaccggt tgaataaaac caatgcatct gcaccggcat ttttcatcct
    11881 taaagacata ttacccattg cactgaaata aggattcagc tttaccgcta ccggaatttt
    11941 gaccgattgt tttatggtct caataatatt caggtaccga tgctccactt cggaagatga
    12001 catagctata tcacccggaa tgaaaaatat attcacttca ataccatcag ccccggcctg
    12061 ttccataagt ttcgagtaat cgatccagcc ttctgtggta ataccattca ggctgccaat
    12121 gatgggaata tttactctct ccttggcctt tcggatattc tccaggtatt catcggtacc
    12181 tacattaaaa tcatccaggt cggggaaata gtccagggct tccggaaaag catatgtggt
    12241 ttccgacata acacctttga acctggcttc ttctttacgg atttgttctt caaataagga
    12301 gaaaagaaca acagcacctg caccattatc ttccatcttt actatattat ctgtttgctc
    12361 agataaagta caggcagata ccaggatcgg ggaatttaat tttaacccca tgtaagttga
    12421 atgcaatttc atatctgtgt ttttaagcta ttggaacaaa atcacttgct tttttagtgg
    12481 ccagttcctc atatgttttc catttcaggt tcaccagttc ctgggcatgt ttcattaacg
    12541 tatcagcagc ttcaggattt gttatggtca gcactttata ccgcaattca ttatacgcat
    12601 actctttcaa accgatagtt ggtcttggtg aatccagtac aaacgggttt tgatttttca
    12661 ttcttaatgt cggattgtaa cgtaataatg gccagtgtcc actggcaaca gcattatgct
    12721 gctggtccaa tccatttttc aaatcatatc catgtgcaat acaatgactg taggctaaaa
    12781 tcaatgcagg cccgtcataa gcttctgctt cccgcatggc cagtaatgtt tgttgtggat
    12841 tagcaccaaa agcaaccctt gccacataca cgttgccata agaaatagcc tgcatggcaa
    12901 gatctttttt acctcctgat ttaccaccgg cagcaaactt ggcagtagcc gcagttggag
    12961 ttgattttga actctgacca ccggtattgg aatacacttc tgtatccagc accagtattt
    13021 tgacatttct gccgctggcc aatgcatgat caagaccacc ataaccaata tcataagccc
    13081 atccatcacc acctaccaac catacactgc gacgtaccag ctggtcagca acagataaca
    13141 aatgaatggc agccggagaa gtcatcgtct tcagtttctc tttcaacaac tgcactcttt
    13201 tttgctgttc tgccagttct gattcatgta tttgtttagc atttattatt tcattgacaa
    13261 aactttcacc cagtacatct ttcaattcca gtaaatactg tttcgcaaca cctacttgtt
    13321 tatcggctgt tatccgcatg cccaatccaa attcagcatt gtcttcaaac aaagagtttg
    13381 accatgccgg acctctgcct tccttgttca cagcccacgg tgtggtagga agattacccc
    13441 cgtaaataga agaacagcca gtagcatttg ccactaataa cctgtcacca aataattgtg
    13501 ataatagttt gagatatggt gtttcgccgc aaccggcaca agcacctgaa aattcaaata
    13561 acggttccag gaattgtgca ccgtgtacag tagaaaaatc aatttttgaa cgatcattcc
    13621 acggaatgtt ttcgaagaat tcgatatttt tttgttcggc ttttaatacc ggctccttct
    13681 cagccatatt gatcgctttc agagaacggt tggccggatc ggtggccgga caaacttcca
    13741 cacataaatt acagccggta caatcttcca ggtatacctg cagtgagtat tgtgtctccg
    13801 gaaaacccct ggcattgata ggtgcagatt tgaaatctgc aggtgctttt ttcaaatggt
    13861 ctttatggta gaacttcgct ctgatcacac tatgcggaca tacataagca caatttccgc
    13921 attgtataca aatattgggt tcccaaattg gcaccaggtc tgaaacattt ctcttttccc
    13981 attgcgtagt tccactggga taagtgccat caacagtcat tttactaaca ggcaaatcat
    14041 caccatgacc cgccatcatc acagctgtaa cttcttttac aaagtcagga gctttatccg
    14101 atacagtttg taaatcatta tcagctgctg aaaccttttt tggatagtcc acttcatata
    14161 agttggccaa tgtagcatct accgctttaa agttctgatc aattaccttc tgtccttttt
    14221 tgaaataggt tttctcgatc gcttttttaa tttgtttgat agcatcttcc cgcggtaata
    14281 cacctgataa agcaaaataa caggtttgca tgatagtatt aatgcggccg ctcataccgg
    14341 tatcccttgc cactgttgtg gcatcaatta catacagttt taattttttg tcaatgatac
    14401 ttttttgtac ctgtcctgtc agcttatccc atacttcatc tttactatat ggactattca
    14461 acaaaaatgt tgctccgtca ctggcaaatt caagcatctc aactttctcc gtaaagttga
    14521 attggtggca ggcaataaaa tcggctttgg aaataaggta tggggcccgg ataggattgg
    14581 gaccaaatct caaatgtgac actgtcctgg cacctgattt ttttgaatca tacacaaagt
    14641 acccctgagc atacaagtca gtattctcac cgataatttt tattgtattt ttattggctc
    14701 ctacagtacc atcagcaccc agtccaaaga acaaaccttg tctccattca gtttcatcca
    14761 acttataagc agggtcgtac tccaggctgg tgaaagtaac atcatcattg attcctaccg
    14821 tgaacccatt tttaggatgc tttttttcca gttcatcata cacccctttt accattgccg
    14881 gtgtaaattc ctttgaagaa aggccataac gtccacctac tatattcggt aattttttca
    14941 gtttgccctg ctgtaatgct tcaactaatg ttgccagaac atcctggtac ataggctcac
    15001 ctgccgcgcc ggattccttc gttctgtcca gcactgcaat tgattttaca gaagccggta
    15061 atacttttag taagtgatcc agcgaaaaag gcctgtataa acgaaccacg atgacaccgg
    15121 tttttctacc ggatttgttt aatgctttca cggtttcttc caccgtttca ctaccagatc
    15181 ccatgatgat ggttatcgct tcagcatccg gggcgccgct gtattcaaag agttcatatt
    15241 ttctgccggt catctttgca aaatcattca tcacttcctc aacaatggat ggcatagcat
    15301 tatagaactt attcacagtt tctcttccct ggaaatacac gtcaggattt tgtgcggttc
    15361 ccctgatgaa gggatgttca ggattcaggc ccctgtttcg atgggcaatc accagttcat
    15421 ccggtatcat cgattttatc tgctcgtcac tcagcatctc cagcttattc acttcgtgtg
    15481 aggtacgaaa accatcgaag aagtgaacga tgggtattct tgacttcaag gtggccacct
    15541 gtgcaatcaa tgcaaaatca tgtgcttcct gcacactggc ggaacctaac atggcaaagc
    15601 ctgtagttct tgcggccatt acatcctggt gatcaccaaa aattgacaga ccctgcgccg
    15661 ctaaggaacg ggccgccaca tgaaatactg caggtgtcaa ttcgccggca atcttgtaca
    15721 tgttcggcaa catcagcatc agcccttgtg aggctgtaaa ggttgttgtc agcgaaccgg
    15781 tttgtaaagc accatgaact gttccggctg caccggcttc actctgcatt tccattacat
    15841 ctggtacagt accccaaata ttttttattc ctttagaggc ccattcatca gccagttcag
    15901 ccatagtaga tgacggggtg ataggataga tggcacaaac ttcattaaca cggtaggcta
    15961 cataagcagc ggcttcgttt ccatcgatcg ttgcgattat ttttttatta cttgacatca
    16021 gtagttgaat taacaggttc tggaatcatt tctatcgcat ggcagggaca ttgttcataa
    16081 cataccgcac aaccagtgca ggcattataa ttgaacttat agcggttccc cttccctagt
    16141 ttaataattg catcctcagg acaggcgccg aagcaaccat cacattcaaa acagttacca
    16201 cagcttaagc aacgctgcgc ttcaaacctt gcttccggtt cagacaaacc cgtgatcact
    16261 tcttcaaaac ttttcactgc cacagcaggt gccagtttat cctgttcttt ttgcggagca
    16321 tcagttttgt accacatatg cagcttccga taactggctg ttggatgctt ttccggtttt
    16381 tgatatggct cctgcatcag gtaaccattt atatatttag ctgctttttt accatgacca
    16441 attgcgattg ttgaacttct gttctccccg ggcagcatat ctcctcctgc aaaaattcct
    16501 tcatacccgg tcatccgttc tgcgtttatc gctactgttc catcttcatt gaccactata
    16561 cctgctactg aacgcaagaa accagaatca gattcctgcc tgttggcaat aatcagtaca
    16621 tcagcatcag tcgtttcaaa ttcaccggta ccaacagcct ttcctttttc cactttcatc
    16681 ttctccagcg tcattttgta cttttcaatt ttactgatac tcctgaaaag ctgcacatcc
    16741 acaccttcgg ccagtgcatc ttcggtttca taatcatagg ctggcatcat tttattatca
    16801 cccggaaaat aaacggatac ttctgagcca aatcttttta tcatccgtgc caggtacaag
    16861 gccagtttac caccaccata caccaccact ttttttctta cataaggtga cgtatttgat
    16921 ttcacttcat tgaaaaaaga aaacgcatca gtgatataaa cagaatcatc atgctgaaaa
    16981 tcttctttac gtattaattg tgccccgata gataagtaga cggcatcaaa attgccggcc
    17041 tgcttttcgg taagcacatc ctgcacttta taattcagcc tgatctttac tcccatcttt
    17101 acaattcgat ctacctctgc atccagaatt tcttttggta accgataatc aggaacaccg
    17161 gtccacaaca aaccaccagg atgtccgcct gcatcatata cttctactgt gtgacccatg
    17221 cgggccaggt gataggctgc tgacaaacca ccggggcctg ctccaaccac taaaaccttt
    17281 ttttcagtgg aattcacaac aaattgaaca ggccattttt gtttgatggc ctcatcaccg
    17341 atatatcttt ctacagcatg aatgtttacc gtatcatcaa tgtgattacg gttgcaccct
    17401 gtctcgcagg gttttacaca aacccggccc ataacggcgg gaaatgggtt gtcttccacc
    17461 aatgtttgga aagcctcgaa ataatttccg gactgtgcat gtgccatcca ggcctggata
    17521 ttttccccgg caggacaggc actattgcag ggaggcataa aatctacgta aacaggtcgg
    17581 cgtaatcgtt tggggccggt gccttccgca tgtatcgaaa ggtcgaccgc tttggtaatg
    17641 tctgttgcag ccatatttaa agttttgcgg gtaagcgtta aacttaccag tgaatagcct
    17701 gattaaagct aaataaacct cagcaaagta gggctgtaaa cgaaagcgaa aggtgacata
    17761 gttcaagcga aaaaatgatt cctatcatgg tattggacga ttaatatgtc aggtgaccag
    17821 ggcaggtggt caataaaaaa ccggcttatc aaggccggct taaaaaatac tattctttgc
    17881 tttatcagaa actataacca acacttactc caccggaagg ttgaatataa ccattggcga
    17941 tcagtggcgt gaacccaatc cgccaggtaa atccaccttt gattggctga cgcctgtaca
    18001 tgaaggatgc tgttccaaaa acattggaag ttttatcatc ataaaaatta aaaatatcca
    18061 gctttctgcc tacataagta ataccggcac ctacttcaaa agtgtgtggt gaatctcctt
    18121 tcccaaaaat ataattgacc tgagcaggaa cggtgacaac agatctcaat tcataattat
    18181 aattaccaag gtcttcatca gcagttacaa aacctagtcc gattcggcct ccccatccca
    18241 gggatgattt ggcaaaacgg gtatcaatat tggccgaaaa taggattccg ggaccaccga
    18301 gttcagcata gaattgtgtc ctgccgggta ctttgccaac attacctggt tgtgcaaaca
    18361 aaacagaact ggttattata gccagggaaa aaattaaaca tcttttcata ataatggttt
    18421 tagcaacaaa gacaaacaca ttagatcttt gctgcttgta caatcagaaa gaaatgttaa
    18481 gctattatag aaaaggtcag aggcttgcca gaacaatatc tttaatctta tcaaccggta
    18541 ccctttcctg ttccatggaa tcccggtgac ggatagtaac tgtattatct tctttactct
    18601 ggtgatcaac cgttacacag aaaggagtac caagagcatc catccggcgg tatcgtttac
    18661 caatagaatc tttttcttca taaaagcatc ggaaatgggg cttacaatct ttcatgatca
    18721 atgctgctat ctccggcaat ccgtctttct tggttaatgg caatacggcc agtttgattg
    18781 gtgccagttt aggcggaaac ttcaatacca cacggctgtc ttgtttctcc gcagtactta
    18841 aatcctgttc ttcatatgcc tggcttatca ccgcaagaaa catccggtcc aaaccaatag
    18901 aagtttcaat gacataagga atgtaattgc caaaaggctt gcccgtagcc gggtcaatgt
    18961 cattatcgaa gtattgttgc ttctttttgc tgtattcctg gtgttgctta agatcgaaat
    19021 cggtacgact gtgaattcct tccagttctt tgaaaccaat tgggaattca aattcaatat
    19081 cacaggcggc atctgcataa tgggccagct tcacatgatc atgatagcgg tatttttcag
    19141 ccgggatacc aagactctgg tgccatttca gtctttcagt tttccagaac tcataccatt
    19201 ctttttgtgt gccggggcgg ataaagaatt gcatctccat ttgctcaaac tcccgcatgc
    19261 ggaaaatgaa ttgcctggct acgatctcgt ttcgaaacgc cttaccaatt tgtgcaattc
    19321 caaacggaat tttcattcgt cctgtttttt gcacattcag gaaattgaca aaaatacctt
    19381 gtgctgtttc aggacgcaga taaactttat tgtcctcatc atcgctggaa acagtagacc
    19441 cgaattcggt ggagaacatc aggttgaatt gacgaatatc tgtccagtta caagtaccac
    19501 ttatggcaca ggaaatctta ttgtcctcaa tcagtttttt taacccttca aaatcatctt
    19561 tggcaagcag tgcatccatg gaagccaaca ggtcatcagc tttttgcatt tggttttctg
    19621 ctttcagcat ttcagcatag ccttctatca aatgatccac ccggtatctt ttcttactgt
    19681 ctttattatc aatcatcggg tcgttgaaat tatcaacatg cccggatgct ttccaggtag
    19741 aggggtgcat aaagatagcc gcatcaatcc ctacaatatt atcttgtaac tgggtcatgc
    19801 ttttccacca gtaatctttg atgttctttt tcagctcact gcccatctgg ccataatcat
    19861 atactgcctg cagaccgtca tatatttcgc tgctgggaaa aataaaaccg tactctttgc
    19921 aatgcgagat gatcgcctgg aacttattcg tatcgtttgc cataaggcgg caaaaataag
    19981 cccaaatgat gacttacagt tttctttacc tttgctttac gctaaaaaaa tctttatgaa
    20041 aaattctgtg ctttttatgg ctatcccggc cactttgatt gctgcctttc tttttacaaa
    20101 tcaacc
//
LOCUS       DAORTI010000060        19539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2401_length_19539_cov_7.763806, whole genome shotgun sequence.
ACCESSION   DAORTI010000060 DAORTI010000000
VERSION     DAORTI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19539
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_19539_cov_7.763806"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLO70_09135"
     CDS             <1..1166
                     /locus_tag="PLO70_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ74670.1"
                     /translation="IYNFTIGDFDASIFPIPKELEDAIVEAYRKHFTNYPAAEGNLDL
                     REAIHSFIKDEEGLDYGINEILVASGGRPLIYTVFRAICDKGDKIIYAVPSWNNNHYT
                     HFVEGEHIVIEAKAENNFMPTADDIRPYVKEASLISLCSPQNPTGTTFRKEELEAICD
                     MVIEENKRRGDGQKKLYVMYDQMYWHLTYAGIKHYNPVSLRYEMRDYTIFIDAISKVF
                     AATGVRVGWGMGPAPVISKMKAILTHIGAWAPMAEQKAVAYYLGNRDGIKKYLANFKN
                     EIEERLRRIYNGFTLLKNEGLPVDAITPEAAIYLTIKIDLAGKKTTEGKVLETQSDVT
                     AYVLNAAGLAVVPFYAFGADRSSAWYRLSVGTCKKNEIEEMIGRLREALKNLS"
     gene            complement(1216..1458)
                     /locus_tag="PLO70_09140"
     CDS             complement(1216..1458)
                     /locus_tag="PLO70_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74671.1"
                     /translation="MKNLNRNLLVLFKDEFMTEQAIEREVSYLNEILSVTDTPEKFCA
                     ANELVDRNCITSNKKKMLKEANHFRLRPFRFFINKN"
     gene            1663..2802
                     /locus_tag="PLO70_09145"
     CDS             1663..2802
                     /locus_tag="PLO70_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ74672.1"
                     /translation="MSQKDTKINPNQPVTYKKHKKPRILVAPLDWGLGHASRCIPIIR
                     ELLELDCEVWLAGEGIQGELLKTEFPELPFLHLPGYTIHYSKTKKGLPWKMIRQSRKL
                     LAAFQSENMWLKKVVPEYSFDAIISDNRFGLFHNTIPSVFITHQLLIKTFAGKWTEKI
                     LQKINYKYINRFTECWVPDIETENSLSGELSHPDKKPEIPVRYIGLLSRFEKKEAVIQ
                     QNHLLIILSGPEPQRTILEEKIIKEVSNYNGTATIVRGLPGSTTLIPSTNMIQIFNHL
                     PAIKLNEEMLKAEYVISRSGYSTVMDIMTLQKKSILIPTPGQTEQEYLARYYRKNNMA
                     YIAGQKDFSLVNSIEKAKQFPYHLPAINPGKKLHTVMQQFLSTLK"
     gene            2882..3739
                     /locus_tag="PLO70_09150"
     CDS             2882..3739
                     /locus_tag="PLO70_09150"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQZ74673.1"
                     /translation="MTNLFFATNLSMVKHISPALVGPYGLNILRVGISLLLFWVVWLF
                     GKTPAGIQQKHLPRFLLCGLTGVAINQMLFIKGLTMTSTIHASLLMLCTPLLITIFAF
                     WILKEKLTILKSLGLALGIGGAVLLIMSKEKNGSASLTGDMFIVLNAMAYTFYFILVK
                     PLMEEYPPLHVVRWVFTFGFVMILPFGWIQFNEIQWFQFEWTHLASLSMIVFCGTFLA
                     YSFNVFGIKHLGAGITGSYIYTQPVFAAIIAILFLHEQFTWEKLLAGILIFGGVFLVS
                     QKTPPVVEE"
     gene            complement(3747..4241)
                     /locus_tag="PLO70_09155"
     CDS             complement(3747..4241)
                     /locus_tag="PLO70_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQZ74674.1"
                     /translation="MKTLLLIRHAKSDWANTALDDFDRPLNERGKKDAPEMADRLKDK
                     KIMIDAFITSPAKRAAKTAKVFAEKYDVKKEGLIIKEEFYLPAPDVFYSVIEKTDDTL
                     TNIAVFSHNNGITDFANMLTETRIDNIPTCGVFAIKIESDSWKNFREAKKEFWFFDYP
                     KAGR"
     gene            complement(4302..6149)
                     /locus_tag="PLO70_09160"
     CDS             complement(4302..6149)
                     /locus_tag="PLO70_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQZ74675.1"
                     /translation="MSDPIKHECGLAFIRLRKPFSYYQQQYGTVLWGLNKLYLLMEKQ
                     HNRGQDGAGVAAVKLNVEPGYPFMNRIRSNAPQAIADIFQQIGKEIEELEKYHPNIKN
                     HPGLMKGHVGYLGELLLGHLRYGTQGKNKLEYCHPFINRDTIPSRNLALAGNFNIVNS
                     DELFTLIGKEPNENVRFSDLAAMIELMHTFLCKEDEVSPGKMDIKKVLKKTLPLLDGG
                     FHVGGVTGNGIGFVFRDAHGIRPSYYYVNDEVVVAASERAAIRTAFNVGENEVQELMP
                     GQALIVDENGTIEIAQILEPKERKACSFERIYFSRGSDEKIYRERNALGYNLSEQVLN
                     AIDHDLKNTIFSFIPNTAETAFYGMLKGMEDYLNKVKIERILSWGKDYDADKLGEMIN
                     RRIRIEKIAIKDVKMRTFITEDTGRNEMVQHVYDITYGTVRPGLDTLVVIDDSIVRGT
                     TLKESIIRMLGRLMPKRIVVVSSSPQIRYPDCYGIDMSKMGDFIAFNAAVELMKERGK
                     LDFMKDMLEKCKDLQRNNQLHTENVVKQLYKQFTTEEISDKIARLITPSGLDIPVHVI
                     FQSIEDLHKSCPNNLGDWYFTGNYPTPGGNKVVNKAFMNYMEGKNVRGY"
     gene            complement(6206..9223)
                     /locus_tag="PLO70_09165"
     CDS             complement(6206..9223)
                     /locus_tag="PLO70_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQZ74676.1"
                     /translation="MNNLAEYFHRSGQFYYILFDLKGNCKYVNPLFHKEFSHICPDFC
                     DHKLADCIAEGEVEKFKTVVEESLRNPDEVFRTDVKMKTSPGISICWEISACKEEIGI
                     PNCVQAIGIVKNNKQALAGQAESPFGELPERYRAFEKSAEGLWMFESAEPVSVTDSPD
                     TIIEYWKKKSYLVECNDNLARMYGFSKAEELIGVSLDQMMDFSDSQKVDNLKQFIRND
                     FQTTTVETKEFDRNGKAKYFLNQMTGIVENGKVKRVWGTQQDITEQRQAEQQLLESEL
                     FYRNLFANSLDGVLITDADGVVTFASPSITPILGYGTDEILGKNTFEYVHPDDRQLAY
                     SAFIEERADLATQKFISVRLLKNDGKWIWCMIRGHNLLQNPYVKGMVVYFYDDTRRKQ
                     TEEALMESEKRFRNQAIILNNVTDVIVTTDLNRVVTSWNYIIEKLSGITSEEAVGKPF
                     RQVIETDYSPYTHDQVAEIVFSHGIWRGEVSFTGSDGERKHLLHTVSLLYDDNGKSIG
                     LLGVGRDVTERKKAEAKLLESELFYRNMITYSLDGIVMTDPKGDIKYCSPSIVKISGY
                     EPADLLGHNIFEFVHPEEIQIAIEAFAQEFKKESVLNYLALRLLHADGEWVWCTVRGH
                     NLLDNPTFNSIVIYFTNDSKRKKIEDKLRESERRFRNLIHNLKLGILLQDEQGKMVVT
                     NQAALDMLGLTEDQLLGAALIDPGWKVVHEDGKDFPGETHPVPVVLKTKKPVLDVVMG
                     IYRPHSNDKVWLLVNAEPVLDIEGNIINVICSFADITEQKRLASELIEQEIQKQKQIT
                     QATIDAQEKERREIGKELHDNINQHLNTTRLYLEVAKEKASGEVLEMISLSHKNLAAI
                     INEIRQMSQSLVPPTLGDLGLIESVQDLCDSLRRAHTFTIEFLYRHFNEENLPGNLRL
                     MLFRIIQEQVSNIIRHAQATVIQIKLQSDAEYINLTITDNGKGFIQGTAKNGGLGFSN
                     ISTRAALFNGKVEITAGPGKGCSLSVLIPQFRG"
     gene            complement(9405..10778)
                     /locus_tag="PLO70_09170"
     CDS             complement(9405..10778)
                     /locus_tag="PLO70_09170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQZ74677.1"
                     /translation="MLITKKIFTACLLLLFVVASAQGQPKKVIADKIAGIVGDKIILQ
                     SDIKNSLADIARQGGTVPENGECLLMEQAIISKVLMLQAQKDSLPVTDEEVEADLDNR
                     IRTYINQLGSQEALEEIAGKTIYQIKDDARESVKEQKLAQAMQRKIVENVRITPIEVK
                     AYFEKIPKDSLPFYESELEIGQIISYPKASRELEQYIAGEMNNYKKQIEMKVTTFEQL
                     AKKVSEDPGSRDRGGQYQINRAEKTWDPVFMSTAFRLKEGEISSPVKSKFGFHIIQMV
                     QRNGDDAIVRHILRIPPVTEEEISQARRKLDTVRSKIIAGTLGFNEAASKYSDDEAAK
                     YTGPFITDREGSTFVTIDMLDKEMVGMLGKMKVGEFSQPTPFESEQGKKGVRVVYLKS
                     RSQPHRMNLQDDYSKISNFALEEKKSKTMEKWLKSKLPSYYVMIDPGTAEDCPQLQKF
                     STDTKGF"
     gene            complement(10908..13745)
                     /gene="infB"
                     /locus_tag="PLO70_09175"
     CDS             complement(10908..13745)
                     /gene="infB"
                     /locus_tag="PLO70_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQZ74678.1"
                     /translation="MAELKLPRLLAAAKEFNIGQDTLIAFLIGKGFAKDDLKPTSKLT
                     EDMYRALQQEFQSDKAAKMKSDQLELPKGAQGEAKKKKEDEEISFKKEEKPAPKAPKK
                     EEEPKPVEEKPVQPVKEEEKKEEEVVKLEVPEIEGPKVVKKIDLSAIDSSTRPKKITK
                     VKKEEEPKPAEEKTVKSKAKKKKEDEEVAEEIKPVEEVVAKEEPVPEEQHSVIENIEV
                     EKLTGPKILGKIELPVDSDTRPVKDEKKKRKRIPIEKKDTKREIFINKEEDKRPGGGG
                     GFNRGRGGPGAGGNRRDSRGAVGGGRREEKLIDEKEIQRKIQETQAKLSGGGGKGKNL
                     KAKLRREKRQEMADAMGEATEDNKLQVTEFISVSELANLMDVSFADVISKCMGLGIMV
                     SINQRLDAEVIELVASEFGFDVEFIDMEKHMEMEEEMEEDDEEDLQPRSPIVTIMGHV
                     DHGKTSLLDYIRSANVVAGEAGGITQHIGAYQVELANGKEITFLDTPGHEAFTAMRAR
                     GAKVTDIAVIVIAADDAVMPQTREAISHAQAAGVPMIFAINKIDKDGANPQKIYEQLS
                     QMNLLVEEWGGKFQNQEIAAKKGLNIDKLLEKILLEAEILDLKANPDREATGTIIEAS
                     LDKGRGYVATLLVENGTLKPGDLIVSGQYFGRVKAMFNERNKKEDKAGPSAPAVILGL
                     NGAPQAGEKFKVYEDEAEAKEIAYRRAQILREQGIRTKKHITLDEIGRRLALGSFKEL
                     KVIIKGDVDGSVEALSDSLQKLSTQEILVSVIHKGVGQINESDIVLAEASDAIVIAFN
                     VRPSLQASRLADNAGIEIKMYSIIYNAIEEVKSAMEGMLEPKVQEKIVANVEIREVFK
                     FDKATVAGCIVLDGKIKRDSKIRLIRDGIVIYPTGEGVNAELASLKRFKDDAKEVVSG
                     MECGLTIKNFSDIKVGDVVEAYEIEEVKRTL"
     gene            complement(13854..15101)
                     /gene="nusA"
                     /locus_tag="PLO70_09180"
     CDS             complement(13854..15101)
                     /gene="nusA"
                     /locus_tag="PLO70_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQZ74679.1"
                     /translation="MSSINLIDAFQDFKDAENIDRPTMMKVVEDVFKTLLRKKYSSDE
                     NFDVIVNAEKGDLEIIRRRMIVEDGQVEDPLAQVAYSDAVKIEPDFEVGEELYEEIDL
                     LDFGRRAILAAKQTLASRISDLKKNVLAKKYGDRIGEIISAEVYQVWKKEILLLDEEG
                     NELILPKSEQIPQDYFKKGENIRAVVRKVDMKNNNPVIILSRTSPDFLAKLLEIEVPE
                     IFDGLIVIKKIVRDPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTSNIQLLIQRSLTPAKITSMELDNENKHANVYLKPDQVSLAIGRRGVNIKLASEL
                     TGYELDVYRDNEGEVEEFDIDLEEFSDEIETWIIDELKRVGCDTARSVLALTPEELER
                     RTDLEKETIDELRKVLKEEFEKE"
     gene            complement(15129..15599)
                     /locus_tag="PLO70_09185"
     CDS             complement(15129..15599)
                     /locus_tag="PLO70_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HQZ74680.1"
                     /translation="MNIESQIQAIEQRVKALISAEPELFLVEIRIKPTNNIKVYIDGN
                     HGVSVDRLVQYNRKLYKQLEEEGMFPEGDFSLELSSPGLDEPLKLHRQYLKNIGRYVE
                     VTGKEGKRVEGKLISATDTEIVVEEVKGKGKKMETVQHTISFDDIKTTKIQIKF"
     gene            15781..16014
                     /locus_tag="PLO70_09190"
     CDS             15781..16014
                     /locus_tag="PLO70_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74681.1"
                     /translation="MKYILFAILAYILYQFIFKLVIPVYLASRKIKKGFREMHSRMQE
                     QMQEQQGFSSQTSPSEPPPKKKAGDYIEFEEIK"
     gene            complement(16015..16791)
                     /locus_tag="PLO70_09195"
     CDS             complement(16015..16791)
                     /locus_tag="PLO70_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ74682.1"
                     /translation="MKKFIALVVLTGLSVLTTAQDLNLFQKKQFISGNDTLPYRLLLP
                     ENYDVSKKYPLVFFLHGAGERGNDNEKQLLHGAKLFLKEEVRKKYPAIVIFPQCPQNS
                     FWSNVDFKMENGKRVFAFKAEGEPTIAMKLAQELLFTILKDYRVSKRKVYVGGLSMGG
                     MGTFEIVGRNPRLFAAAFPICGGGEPSTASQLKKTKWWIFHGGKDDVVPPELSQKMTV
                     ALKAVKASVKFTLYPTANHNSWDSAFAEPGLFPWLFAQKK"
     gene            complement(16797..19034)
                     /locus_tag="PLO70_09200"
     CDS             complement(16797..19034)
                     /locus_tag="PLO70_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQZ74683.1"
                     /translation="MYNPVFKRSVILFIALVSFIVCTAQKKTIDQRVDSVLKLMTLEE
                     KAGQMNQYNGPWAATGPLTNDNNLLSQIKDGKVGSMLNVTGVARTKELQQLAMQSRLK
                     IPLLFGQDVIHGYRTIFPIPLGEAASWDIPAMEQSARIAATEAAAVGVHWTFAPMVDI
                     ARDPRWGRVMEGAGEDPYLGSLIATARVKGFQGKVFGSTDGLMACAKHFAAYGAAVGG
                     RDYNSVDMSLRQLHEVYLPPFKAAAEAGAATFMNSFNDLNGIPATGNKYLQRDILKGE
                     WKFKGFVVSDWGSVGEMINHGFSKDNYEAAMQAVNAGSDMDMESRSYIKHLAALVKEG
                     KVKMNVIDDAVRRILKKKFEMGLFDDPYKYSNEEREKAQWNNAEHIKVARDISRKSIV
                     LLKNEKQLLPLSKQTKKIALIGPFAKAKADNNGFWSYDWPDDTARISSLWEGVQRKLS
                     AASEIVYAKGCNINDSSTVGFAEAVAVAKEADIVIISVGEARDMSGEAKSRSNIHLPG
                     VQEKLIKAVMNAGKPVVVLISAGRPLIFNWTADNAPAILYTWWLGTEAGNAIADVLFG
                     DYNPSGKLPMTFPRTEGQIPVYYNHYNTGRPAKNDSDLHYVSAYTDLPNSPKFPFGFG
                     LSYTKFDYSNIRLSKAQFKPGEKIEAMVTVHNNGNYDGEEVVQLYIRDITGSVVRPVK
                     ELKGFQKITLKKGETKEVRFTISVNDLRFYNDKLQYIYEPGQFKLYIGGSSNDVKEVN
                     FQLTK"
     gene            complement(19101..>19539)
                     /locus_tag="PLO70_09205"
     CDS             complement(19101..>19539)
                     /locus_tag="PLO70_09205"
                     /inference="COORDINATES: protein motif:HMM:NF018828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HQZ74684.1"
                     /translation="YVAGSMVTNFNVPDAGKLTPYTALVIFAIGIVISSFLFNSLQMK
                     RPFIGTPVSFTDYFKGITKDHLIGILGGVIWCVGMSLSIIASGKAGPAVSYGLGQGAT
                     VVAALWGIYVWREFDKAPQGTKSLLNIMLLLYIGGLAMIILSK"
BASE COUNT         5349 a         4550 c         3397 g         6243 t
ORIGIN      
        1 gtatttataa ttttacgatc ggtgattttg atgcctctat tttccccatt cccaaagaac
       61 tggaagatgc tatcgtggaa gcttacagaa aacacttcac caattatccg gcggctgaag
      121 gtaaccttga tttgagagaa gctattcatt cttttatcaa agatgaagaa gggcttgatt
      181 acggcatcaa tgaaatatta gttgcgtcag gcggccggcc tttgatctat acagtctttc
      241 gtgccatttg cgataaaggg gataagatca tttatgcagt tccatcctgg aataataatc
      301 attataccca ttttgtagag ggtgaacata ttgttatcga agcaaaggcc gaaaacaatt
      361 tcatgcctac agctgatgac atccgaccgt atgtgaaaga ggccagtctt atttctttgt
      421 gttctcctca aaaccctacc ggtaccactt tccgcaaaga agaactggaa gctatctgtg
      481 atatggtaat tgaagaaaac aaaagacgtg gtgatgggca aaagaaatta tatgtaatgt
      541 atgaccagat gtactggcat ttaacatatg caggcattaa acattacaac ccggtttccc
      601 tccgatatga aatgagagat tacaccatct ttattgatgc aatcagtaaa gtgtttgccg
      661 caacaggtgt ccgtgttggc tggggtatgg gacctgctcc tgttatcagt aagatgaaag
      721 ccatcctaac tcatataggt gcatgggcac ctatggcaga acaaaaagca gtagcctatt
      781 acctgggcaa ccgtgatgga attaaaaaat acctggctaa ttttaaaaac gaaattgaag
      841 aaagactacg ccggatatat aatgggttta ccctgttgaa aaatgaagga ctgcctgtag
      901 atgccattac tcctgaagca gctatttacc ttaccataaa aatagacctg gcaggaaaga
      961 aaacaacaga aggaaaggtt ttggaaacac aaagtgatgt taccgcttat gtgctgaatg
     1021 cagcaggttt ggcggttgtc cccttctatg catttggtgc tgacagaagt tctgcatggt
     1081 atcgtcttag tgtaggtacc tgcaaaaaga acgaaatcga ggaaatgatt ggaagactca
     1141 gagaagcttt aaaaaatcta tcctaaaaaa aagagagaat ctgttagtct tctcccttta
     1201 attacattct tcaaatcaat ttttattgat aaaaaaccgg aaaggcctta acctgaaatg
     1261 gttggcttct ttcagcattt tctttttatt ggaagtaata cagttacgat ctaccagttc
     1321 attagcggcg caaaattttt caggcgtatc tgttacagat aaaatttcat tcagatagga
     1381 tacttcccgt tcaatagcct gttcggtcat gaactcatct ttgaataaca ctaagaggtt
     1441 tctgttgagg ttcttcatta atagacaggt tttaatatcc tgacattcct tttgtactcg
     1501 gacagccaca gttttttcca cctttgccgc taccagaata ataattccgg ttacaagcgg
     1561 ctaaaaccat aactaataac agggctatcg ctattttgtt gattgacttc atatattgga
     1621 tgttctaaat taaaactatt ttacaaacaa attagtatcc cgttgagcca aaaagacaca
     1681 aaaattaatc caaatcagcc tgttacgtat aaaaaacaca agaaaccccg cattttggta
     1741 gccccgctgg attggggact ggggcatgcc tcccggtgca tccctataat ccgggagctt
     1801 ttggagctgg attgtgaggt ttggctggct ggcgaaggca tacaggggga attgctgaaa
     1861 actgagtttc ccgaattacc ttttctccat ttgccgggct atactattca ttattctaaa
     1921 acaaaaaaag gattgccctg gaaaatgatc cggcaaagcc gcaaattatt agccgctttt
     1981 caatcagaaa atatgtggct taaaaaagtg gttcccgaat acagcttcga tgctattatc
     2041 agcgataacc ggtttggttt atttcataat accattccct ccgtttttat cacacaccag
     2101 ttactgataa aaacttttgc cggtaaatgg acagaaaaaa tactgcagaa aataaattac
     2161 aaatatatca accggtttac agagtgctgg gtcccggata tagaaactga aaatagctta
     2221 tcaggtgaat tgtcacaccc cgataaaaaa cctgaaatac cagttcgtta tatcgggtta
     2281 ctgtcgagat ttgaaaaaaa agaagcagta attcagcaaa atcatttact tattatcctg
     2341 tcaggacctg aaccacaacg aacaattctg gaggaaaaaa taattaaaga agtaagtaac
     2401 tacaatggca cagcaactat tgtaagggga ttacccggat caacaacatt gattccatcc
     2461 accaatatga tacaaatatt taatcatctg cctgcaataa aactgaacga ggaaatgttg
     2521 aaggcagaat atgtcatcag ccgcagcgga tatagtacgg taatggatat tatgaccctg
     2581 caaaagaaaa gtattttaat ccctacaccc gggcaaactg aacaagaata ccttgcccgt
     2641 tattacagga agaataatat ggcatatata gcagggcaaa aagacttttc cttagttaat
     2701 tcaattgaaa aggcaaagca atttccttat catctgccag caattaaccc tggtaaaaaa
     2761 ttacacacag tgatgcagca attcctgtcc actttaaagt aatcttttcc tttttacctt
     2821 tacacagttt ctaaaatttt ctaatctgaa taccagggcc aaagcacata ctgctgtatt
     2881 actgacaaat ctgttctttg ctactaacct gagtatggta aaacatattt caccggcact
     2941 ggttggcccg tatggattaa atatccttcg tgttggcatc agcctgttgc tattctgggt
     3001 cgtttggctg tttggtaaaa caccggcagg cattcagcag aaacacctgc cccgctttct
     3061 cttatgtggt ttaactggtg tggccattaa ccagatgctc tttattaagg gcttaacgat
     3121 gacttccact attcacgcat ccttactgat gctttgtaca cccttgctga taaccatctt
     3181 tgcattttgg atattaaaag aaaagcttac tattcttaaa tcgctgggac tggcattggg
     3241 tataggcgga gcggtcttat taattatgtc gaaagagaaa aatggttcag cctcactgac
     3301 cggcgacatg ttcattgtac tgaatgccat ggcttatacc ttttatttta tcctggtaaa
     3361 accattaatg gaggaatacc cgcccctgca tgtggtaagg tgggtcttca cttttggttt
     3421 cgtcatgatt cttccttttg gctggataca gttcaacgaa attcaatggt ttcagtttga
     3481 gtggacacat cttgcctcac tttccatgat tgtattctgt ggtacttttt tggcttactc
     3541 attcaatgtt ttcggaataa agcatttagg tgcagggatt accggttctt atatttatac
     3601 acaaccagtg tttgcagcca ttatagcaat ccttttctta cacgaacaat ttacatggga
     3661 aaaattactg gctggtatac tgattttcgg cggagtattc ctggttagcc aaaaaacacc
     3721 accagtagta gaagagtaaa ccagttttac cggcctgcct ttggataatc aaaaaaccaa
     3781 aattcctttt tcgcctccct gaaattcttc cagctatcac tttcaatttt tatggcaaaa
     3841 actccgcagg tgggaatatt atcaatcctg gtttccgtaa gcatgtttgc aaaatcagta
     3901 atcccgttat tatgagaaaa gacagcaata ttggttaaag tatcatctgt tttttcaata
     3961 acagaataga atacatcggg agcaggaaga taaaattcct ccttaataat taacccctcc
     4021 ttttttacat catacttttc agcaaagact tttgcagttt tagctgccct ttttgcagga
     4081 ctggtaataa atgcatcaat cattattttt ttatccttta aacggtccgc catttccggt
     4141 gcatcttttt ttcctctctc attaagtggc ctgtcaaaat cgtccaaagc ggtatttgcc
     4201 cagtcacttt ttgcatgacg aataagcaat aatgttttca tcctataaag tttattgtaa
     4261 agtaatgaat tttgccaccg cctgttctgg ccgggtatat tctaatagcc tcttacattc
     4321 ttcccttcca tataattcat gaaagctttg ttgacaacct tatttccgcc gggagttgga
     4381 taattaccgg taaaatacca gtcaccaagg ttattagggc aggatttgtg aaggtcctca
     4441 atgctctgaa atattacatg cacaggtata tcaaggccgg atggagttat taaccgggca
     4501 atcttatcgg agatctcttc cgtagtaaac tgtttataga gttgctttac cacattctct
     4561 gtgtgtaact gattattgcg ttgcaggtcc ttgcattttt ccagcatgtc tttcataaaa
     4621 tccaacttac ctctttcttt cattaactca actgcagcat taaaagcgat aaagtctccc
     4681 attttgctca tgtcaattcc ataacaatca ggataccgga tctgtggcga ggaggagaca
     4741 acgacaattc gttttggcat caaccttccc agcatacgaa taatgctctc cttcagcgtg
     4801 gtgcctctta caatagaatc atcaattacc accagggtat ccagtccggg acgaacagtt
     4861 ccataggtaa tatcatagac gtgttgcacc atttcgttac ggcctgtatc ttccgtaatg
     4921 aatgtccgca tcttcacatc tttaatagca atcttttcaa tgcggatacg gcgattgatc
     4981 atttcaccca gcttatctgc atcatagtct ttgccccagc tcagtatcct ttctatttta
     5041 actttattca aataatcttc catccccttc aacattccat agaaggctgt ttctgctgta
     5101 ttcggtataa atgaaaaaat agtattcttt aagtcatgat cgatagcatt taacacctgc
     5161 tcactcagat tatatccaag tgcatttctt tccctgtaaa ttttttcgtc actgcccctc
     5221 gaaaaataga tcctttcaaa actacaggcc tttctttctt tgggttccag tatctgggcg
     5281 atttcaatag ttccattttc gtccacaatc aatgcctgtc cgggcattag ctcctgcact
     5341 tcattttctc caacattgaa ggcggttctt atggccgccc tttcagatgc agcaactacc
     5401 acttcatcat ttacataata ataggaaggc cggataccat gagcgtcacg aaaaacaaaa
     5461 ccgataccat tccctgttac accccctaca tgaaatccgc catccagaag gggtaacgtt
     5521 tttttcaata cttttttgat atccattttt ccgggtgaaa cttcatcttc tttgcacaga
     5581 aaagtatgca tcagttcgat cattgcagcc aggtcactaa agcggacatt ctcatttggc
     5641 tctttaccaa tcagggtaaa taattcatcg gagttgacaa tattaaagtt tccggctaaa
     5701 gcaaggttgc gggaaggtat ggtatcacga ttgatgaaag gatggcaata ttccagcttg
     5761 tttttaccct gtgttccata gcgcagatga cccaggagca actcaccaag ataacccaca
     5821 tgccctttca tcaggccggg atggttctta atgttggggt gatacttttc caattcttca
     5881 atttcctttc caatttgctg aaagatatcg gcgattgcct gcggtgcatt actacggatg
     5941 cggttcatga aggggtaccc gggttctaca ttcagtttga cagccgccac accggctcca
     6001 tcctggcccc ggttatgctg cttttccata agcaggtaca gcttgttgag cccccacaat
     6061 acggtaccat actgctgctg gtaataggag aaaggctttc ttaaccggat gaatgccagt
     6121 ccgcattcgt gtttgatcgg gtcactcatg gaagccgcaa aggtaagcga taaatagtaa
     6181 ctaaaatgcc taattagccg gctctttacc ccctgaattg tgggatcaat acagaaaggg
     6241 aacaaccctt accgggccct gccgttattt ccactttccc attgaaaagc gctgctcttg
     6301 tgctgatatt actaaaacct aaccccccgt ttttagcggt accctggata aatcctttgc
     6361 cattatctgt gatagtaaga ttgatgtatt cagcatcaga ttgaagttta atctgaataa
     6421 ctgtagcctg agcatggcgt ataatattac ttacctgctc ctggataatg cgaaacaaca
     6481 tgagcctgag attgccggga agattttctt cattaaaatg acgataaaga aactctattg
     6541 tgaatgtatg tgcccttctc aatgaatcac acaggtcctg tacagattcg atcaacccta
     6601 aatctccaag tgttggagga accagtgatt gtgacatctg cctgatttca ttaatgatcg
     6661 ctgccaggtt cttatgagaa aggcttatca tttccagcac ttctccggaa gccttttctt
     6721 ttgccacttc caggtagagg cgggttgtat tcaggtgctg gttaatatta tcgtgtaatt
     6781 ctttaccgat ctctcttcgt tctttttcct gtgcgtcgat agttgcctgc gttatctgtt
     6841 tttgcttttg gatctcttgt tcaattaatt cactggcaag tcttttttgt tcggttatat
     6901 ctgcaaaaga acatattaca ttaataatgt ttccctctat atccaaaacc ggctctgcat
     6961 tcaccaatag ccaaaccttg tcattcgaat gcggccggta tatacccatt acaacatcca
     7021 ggactggttt ttttgttttg agaactacag gtaccggatg agtttcccca gggaaatcct
     7081 ttccatcctc atgtaccact ttccatcccg gatctatgag tgcagcgcca agcaactggt
     7141 cttctgttaa accaagcata tccagtgctg cctggttggt aactaccatt tttccctgct
     7201 catcctgaag taatattccc agtttaagat tgtgaatcag gtttctgaat cttctttcac
     7261 tttccctgag tttgtcttca attttttttc tttttgaatc atttgtaaaa taaatgacta
     7321 tagaattaaa ggttggatta tccagtaaat tatgccccct taccgtacac caaacccatt
     7381 ctccgtccgc atgcaggagt cgcagagcaa gataattaag cacagattct tttttaaact
     7441 cctgcgcaaa cgcttctata gctatttgaa tttcttccgg atgtacaaat tcaaaaatat
     7501 tatggcctaa gagatcagcc ggttcatatc ctgatatttt tacaatggaa ggactgcaat
     7561 attttatatc cccttttgga tctgtcatta caattccatc cagggaataa gtaatcatat
     7621 tacgataaaa aagctcactt tccaggagtt ttgcctctgc tttctttcgt tcagttacat
     7681 ctcttcccac ccctaataag ccaatgctct tgccattatc atcatacaaa agagaaacag
     7741 tatgcaataa atgtttccgc tccccgtcac ttccggtaaa tgatacttca cctctccata
     7801 ttccatgcga aaaaacaatt tcggccacct ggtcatgtgt atacggagag taatcagttt
     7861 cgattacctg tctgaatggt ttgcctactg cttcctccga tgtaataccg ctgagctttt
     7921 caataatata gttccacgaa gtgacaaccc tgttcagatc agttgttaca attacatccg
     7981 taacattgtt gaggattatt gcctggttgc ggaaacgttt ttcactttcc ataagtgctt
     8041 cttccgtttg cttccgcctg gtgtcatcat aaaaatatac aaccatcccc ttaacataag
     8101 gattttgcaa aagattatgc cctcttatca tacaccatat ccatttgcca tcattcttta
     8161 gcagccgtac cgaaataaat ttttgtgtag caaggtcagc ccgttcttct atgaatgccg
     8221 aatatgccaa ctgcctgtca tcggggtgaa catattcaaa agtatttttt cctaatattt
     8281 catcagtgcc atagcccaat atcggggtaa ttgaaggaga agcaaacgta acaactccat
     8341 ctgcatctgt aataagtact ccgtccaaag aattagcaaa aagattccga taaaacagtt
     8401 cgctttccaa taactgttgc tccgcctggc gctgttctgt aatatcctgc tgcgttcccc
     8461 aaactctctt cacctttcca ttttcaacaa taccagtcat ttggtttaga aaatatttgg
     8521 ctttgccatt cctgtcaaat tctttggttt caacagtcgt agtctgaaaa tcattgcgga
     8581 taaattgctt tagattgtcc actttttgcg aatcagaaaa atccatcatt tggtccaggg
     8641 aaacgcctat caattcttcc gctttgctaa atccatacat ccgtgccagg ttatcattac
     8701 attcaacgag gtatgatttt tttttccagt actcaataat cgtatcgggt gaatcagtta
     8761 ctgacaccgg ctcagctgat tcaaacatcc aaaggccttc agcacttttc tcaaaggccc
     8821 tgtatcgttc cggtaattct ccaaaaggtg attcagcctg accagctaaa gcttgtttat
     8881 tattttttac aatacctatc gcttgtacac aattgggaat cccgatttct tctttacagg
     8941 cggatatctc ccagcaaata gaaatacctg gagaagtttt catttttaca tctgtacgaa
     9001 agacttcatc cggattccgg aggctttcct caacaacagt tttaaatttt tcaacctcgc
     9061 cttccgcaat gcaatctgca agtttgtgat cacaaaaatc aggacagatg tgactgaatt
     9121 ccttatgaaa aagaggattc acatacttac aatttccttt cagatcaaaa aggatataat
     9181 agaattgccc ggagcgatga aaatattcag ccagattatt cattttgtat gatgtggtaa
     9241 ggattgttac gttttacctt tgccaatgta atcaaccggt taaaaataac tatattaata
     9301 attggctaat gcagttaatt tactgagtcc taaaattatt ttacccgtat taagagaaaa
     9361 gccgctacag ttgtaacggc ttctacttat gtatgtaaat tgatttaaaa tccttttgta
     9421 tcggtagaaa atttctgtaa ttgcgggcaa tcttcagcag ttcccggatc tatcataaca
     9481 taataagaag gaagttttga tttaagccat ttttccatcg tcttactttt cttttcttcc
     9541 aatgcaaagt tggaaatttt gctgtaatca tcctgcaggt tcatccggtg tggttgtgag
     9601 cgggacttca ggtaaacgac acgtacacct tttttacctt gctcactctc aaaaggagta
     9661 ggttgggaaa actctcccac tttcattttt cccagcattc caaccatctc tttatccagc
     9721 atatcgatgg taacaaacgt tgagccctct ctgtctgtga taaaaggacc tgtgtacttc
     9781 gcagcttcat catcactata ctttgatgca gcttcattga atcctaaagt acctgcaatt
     9841 atttttgaac gaacggtatc taattttctt cttgcctggc ttatttcttc ttcagtaaca
     9901 ggagggatac ggagtatatg acgaacaatg gcatcatcac cattccgttg caccatctga
     9961 atgatatgaa aaccaaattt tgatttaaca ggagacgata tttcaccttc tttcaaacgg
    10021 aaagcagttg acataaaaac ggggtcccat gttttttccg cccggtttat ctggtactga
    10081 ccacccctat ccctgctacc cggatcttct gaaacttttt tagccaattg ctcaaaagtt
    10141 gtcaccttca tttcaatctg cttcttataa ttgttcatct ccccggcaat atactgctcc
    10201 agttccctgg aagctttggg ataactgata atctggccta tctccagctc agactcgtaa
    10261 aaaggaaggc tgtctttggg tattttttca aaataggctt ttacttcaat aggagtgatc
    10321 cttacatttt ctacaatttt tcgctgcatg gcctgtgcca gcttttgttc ttttacagat
    10381 tctcttgcat catccttgat ctggtaaata gtttttcctg caatttcttc cagggcttcc
    10441 tggctaccta attggttaat atatgtccga atcctgttgt ccagatctgc ctctacttct
    10501 tcatccgtta ccggcaaaga atctttttga gcctgcagca tcaacacctt tgagattata
    10561 gcctgctcca tcagtaaaca ttcaccattt tccggaacag tgcctccctg gcgagcaata
    10621 tctgccagcg agtttttaat atctgattga agaatgatct tatcgccgac aataccggca
    10681 attttatctg ctattacttt cttaggttga ccctgggcag atgcaacaac aaacaacagc
    10741 aacaaacagg cagtaaaaat tttctttgta ataagcataa gttcaaaagt actttgccgg
    10801 gcaaaactta aatataaccc ggaaggtatt taacaaaagt ttctaagtga cggctacagc
    10861 ctgaaaacaa gatacaagct ttccaggctg tagcttatac cttgatatta taatgttcgc
    10921 tttacttctt caatttcata agcttcaacc acatcgccga cttttatatc actgaaattc
    10981 ttgatagtca atccgcattc cataccagat accacttctt ttgcatcgtc tttgaatcgc
    11041 ttcagggagg ccagttccgc attgacacct tccccggtcg gataaataac gataccatca
    11101 cgtatcaggc gaatcttgga atctctctta atcttaccat ccagtacaat acatccggca
    11161 actgttgctt tatcgaactt aaacacttcc cttatttcca cattagcaac aattttctcc
    11221 tgcactttcg gttccagcat accttccatg gcactcttca cttcttcaat cgcattgtag
    11281 ataatagaat acatctttat ctcaatacct gcattatctg ccaatcttga ggcctgcagg
    11341 gaaggacgaa cgttaaaggc aataacgata gcatcagatg cttcagccag tacgatatca
    11401 ctttcattga tctgaccaac ccctttatgt ataacactta ccagtatctc ctgtgtagat
    11461 agtttttgca aagagtcgct caacgcttct actgaaccgt ccacatcacc cttgataata
    11521 actttcagtt ctttaaaact acccaatgca agacgacggc ctatttcatc cagtgtaata
    11581 tgtttcttgg ttctgatacc ctgctcacgc agtatctgtg cacggcgata tgcaatttcc
    11641 tttgcctctg cttcatcctc gtagacctta aatttttcac ctgcctgtgg tgctccgttt
    11701 aaccccaata ttacagctgg tgcagaaggt cctgctttat cttctttttt atttctttca
    11761 ttgaacatgg ccttcacacg gccaaaatac tggccactga caatcagatc cccgggtttc
    11821 aatgttccgt tctccaccaa taatgttgct acatagccac ggcccttatc cagtgaagct
    11881 tcaataatgg tacctgttgc ttcccgatca ggattagctt tcaggtcaag tatttcagcc
    11941 tcaagcagaa ttttttccag gagcttgtca atattcaatc cttttttggc ggctatttcc
    12001 tggttctgga acttaccacc ccactcttcc acgagtaaat tcatttgtga taattgctcg
    12061 tatatcttct gaggattagc cccgtcttta tcaattttat tgatggcaaa aatcatcggt
    12121 acacctgctg cctgggcatg gctgatagct tcacgggttt gcggcatcac tgcatcatcc
    12181 gctgcaataa caattactgc aatatcggta accttggcac cccttgccct catcgctgta
    12241 aaagcttcgt gaccgggtgt atctaagaac gtaatttctt ttccattagc taattccacc
    12301 tggtaagcac cgatgtgctg cgtaataccg ccggcttcac ctgctaccac atttgcactg
    12361 cgtatatagt caagcaatga tgtctttcca tggtcaacgt ggcccatgat agtaacaatc
    12421 ggagaacggg gctgaagatc ctcttcatca tcttcttcca tttcttcttc catttccatg
    12481 tgtttctcca tgtcaataaa ttcaacatcg aaaccaaatt cactggcgac caattcaatt
    12541 acttctgcat ccagacgctg attgatggac accatgatac caagacccat acatttactg
    12601 ataacatctg caaagcttac atccatcaaa ttagccaatt cgcttacact gataaattca
    12661 gtaacctgca gtttgttatc ttctgtggct tcacccattg catcggccat ttcctgtctt
    12721 ttctcacggc gtaatttggc tttcaggttt ttaccttttc caccaccacc agacaatttt
    12781 gcctgtgttt cctgtatttt tcgctggatc tctttttcgt caatcaattt ttcttcgcga
    12841 cgaccgcccc caactgcacc gcggctatcg cggcgattgc caccagcacc gggtccaccc
    12901 cttccacggt tgaaaccacc accgccaccc ggccttttat cttcttcttt attaatgaaa
    12961 atttcccgtt tggtatcttt tttctctatc gggatacgtt tccttttttt cttttcatct
    13021 tttacaggcc ttgtatcgct atcaacgggt aattcaatct taccaagaat tttcggaccc
    13081 gtcaatttct ccacctcgat attttcaata actgaatgct gttcttcagg taccggttct
    13141 tcttttgcaa ctacttcttc aacaggcttt atttcttctg ctacttcttc gtcttccttt
    13201 ttcttttttg ctttactctt tacagttttt tcttctgcag gttttggctc ttcttccttt
    13261 ttaactttgg taatcttttt aggacgggtt gaagaatcta tagcagagag gtctatcttt
    13321 ttcaccactt taggtccttc tatttcagga acctccaatt ttaccacttc ttcttctttc
    13381 ttttcttctt ctttaacagg ctgaaccggc ttttcttcta ctggctttgg ctcttcctcc
    13441 tttttgggtg ctttaggagc cggcttttct tcttttttga aactaatctc ttcgtcctct
    13501 tttttctttt ttgcttctcc ctgggctcct ttgggtaatt caagctggtc gcttttcatc
    13561 ttagcggcct tatcactttg aaattcctgt tgcaaagccc ggtacatatc ctcagtcaac
    13621 ttagacgtag gcttaagatc atctttagcg aatcctttcc ctatcaaaaa ggcaataaga
    13681 gtgtcctggc caatgttaaa ttctttggcc gcagctaata atcttggaag ttttaattct
    13741 gccattcagt ttatttaatt gtcgttaagt caaatgctgt cataatagta catagcaaat
    13801 aagcaggaaa ttcccaatga cttattttac tacaatgaca ttgctgactt catttattct
    13861 ttttcaaatt cttctttcag cacttttctt aattcatcaa tcgtttcttt ctccagatca
    13921 gttcttcttt ccaattcctc gggagtaagg gccaacacac tcctggctgt atcacatcct
    13981 acccgtttca gctcatcaat gatccaggtt tcaatttcat cactgaactc ctcaagatca
    14041 atatcaaact cttccacttc accttcgttg tcacgatata catccagctc ataaccggtc
    14101 aattcagagg ccagtttgat atttacaccc cttcggccga tcgccagcga aacctggtct
    14161 ggtttaaggt aaacatttgc atgtttattc tcattatcca actccatact agtgatcttg
    14221 gcaggtgtca gtgaacgttg tatcagcaac tggatgttac tggtccagtt gatcacatca
    14281 atattttcat tttttaactc acgaacgata ccatgtatcc ggcttccttt catacctacg
    14341 caggcaccga cgggatcaat acgatcatca tacgattcaa cagcaacttt agctctttct
    14401 cccggatcac gaacaatttt tttgataaca atcaagccat caaaaatttc tggcacttca
    14461 atctccagca atttcgcaag gaagtcgggt gaagtacggg aaagaatgat aacagggttg
    14521 ttattcttca tatccacttt tcttacaaca gcacggatat tctcgccttt tttaaaataa
    14581 tcctgtggta tctgctcact tttgggcaat atcagttcat taccttcctc atccaacaat
    14641 agtatctctt tcttccacac ctggtaaacc tcggcactga tgatctcacc gattctgtca
    14701 ccatattttt tggccagtac atttttcttc aggtcactga tgcggctggc cagtgtctgt
    14761 tttgcagcaa ggatagcccg gcggccaaaa tcaagcaggt caatctcttc gtacaactct
    14821 tcaccaactt caaagtctgg ctctatcttc acagcgtcag aataggcaac ctgcgccagc
    14881 ggatcttcca cttgcccatc ttccacaatc atcctgcgac ggataatttc aaggtcccct
    14941 ttttcagcgt ttacaatcac atcaaaattc tcatcactac tatacttttt acgcaatagg
    15001 gttttaaata catcttccac cactttcatc atggtagggc ggtcaatatt ttccgcatct
    15061 ttaaaatcct ggaatgcgtc gatcaggtta atacttgaca taacacaaag ttttgaaatc
    15121 ccggttgatt aaaatttaat ttgaattttt gttgttttta tatcatcaaa agaaatagtg
    15181 tgttgcactg tttccatttt ttttcctttt cctttaactt cttccaccac aatttcggta
    15241 tctgttgcac tgatcagctt tccttcaacc cttttaccct cttttcctgt tacttcgaca
    15301 taacggccaa tatttttcag atactgccgg tgtagtttca acggctcatc caaacccggt
    15361 gaagacaatt caagggaaaa atctccctcc ggaaacattc cctcttcttc cagctgcttg
    15421 tataatttac ggttatattg aaccagtcta tcaacactaa ccccatgatt accatcaata
    15481 tacaccttaa tgttgttggt aggtttaatt ctaatttcaa ccaaaaacaa ttccggttca
    15541 gcgctgatca gggctttcac cctttgctca attgcttgta tttgagattc tatgttcatg
    15601 gggtataaaa gaagaaggga acggcctcgt tcccttctat cgtctttttc cgatgcaaat
    15661 gtaagggaaa tgaatggtat gagccaaatt gaaaatctga aaaacagaag gttaataatt
    15721 cggtaaacaa cggcaaattc ccggcaatga aaaaaagagc cgtcttatct ttacaccata
    15781 atgaagtaca ttttattcgc catcctggct tatatattat accagttcat ttttaaactg
    15841 gtcattcctg tgtacctggc cagccgcaaa ataaaaaaag gattccgtga aatgcatagc
    15901 cgtatgcagg aacagatgca ggagcagcag ggcttttcat cccaaacttc cccctcagaa
    15961 cctcctccta aaaaaaaggc gggagattat attgagttcg aggaaataaa ataatcactt
    16021 tttctgtgca aacaaccagg gaaacaagcc tggttcagca aaggcagagt cccagctatt
    16081 atggttagca gttggataca acgtaaattt tactgatgct tttacagctt ttaaagctac
    16141 tgtcattttt tgggataatt ccggtggcac aacatcgtcc ttacccccat gaaatatcca
    16201 ccatttggtt ttcttaagtt gtgaagctgt tgaaggttcg ccgccgccac atatagggaa
    16261 ggccgctgcg aaaagcctgg gattacgtcc tactatctca aaagtcccca taccacccat
    16321 ggataatcca ccaacataga cctttctctt acttacccga tagtccttta aaattgtaaa
    16381 caataactcc tgtgccagct tcatcgcaat tgttggttcg ccttctgctt taaatgcaaa
    16441 tactctttta ccattttcca ttttaaaatc aacattactc cagaagctat tttggggaca
    16501 ttgcggaaaa atcacaatag cgggatattt ttttctcact tcctctttca ggaaaagctt
    16561 agctccatgt aacagttgtt tttcattgtc atttcccctt tcaccggcgc cgtggagaaa
    16621 aaataccaat ggatattttt tagagacatc ataattctct ggcagtaata aacgatacgg
    16681 cagcgtatca ttaccactaa taaattgctt tttttgaaag aggttcaaat cctgagcggt
    16741 cgtcagcaca gacaaaccgg tgagtacgac taaagcaata aattttttca taccatttat
    16801 ttagtgagct gaaaattaac ttctttcacg tcattgctgc tacccccgat atacaattta
    16861 aattgtcccg gttcgtaaat atattgcagt ttatcgttgt aaaaacggag atcattcaca
    16921 ctaatcgtaa atcggacttc ttttgtttct cctttcttca aggtaatttt ttgaaaccct
    16981 tttaactctt ttaccggcct tactacagaa ccggttatat ctctaatata taattgcacc
    17041 acctcttccc cgtcataatt accattatta tgaactgtaa ccattgcttc aatcttttcc
    17101 ccaggtttga actgagcttt actcagccgg atattgctgt aatcaaattt tgtatagctg
    17161 agcccaaatc caaatggaaa tttcgggctg ttgggcagat cggtataggc tgatacataa
    17221 tgcaggtcac tatcattctt tgccggacgg cctgtattat aatgattata atagaccggt
    17281 atctgtccct cagttcttgg aaaagtcatt ggtaatttgc cggaaggatt atagtcgcca
    17341 aacaatacat ctgcaatagc attgcctgct tccgtaccca gccaccaggt gtaaagaatc
    17401 gctggtgcat tatctgccgt ccagttaaag atcaatggac gaccggcact gatcaatacc
    17461 acaacaggtt ttcctgcatt catcactgct ttgatcaatt tttcctgcac accgggcaaa
    17521 tgtatattgc tcctgctttt ggcttcacca ctcatatccc tggcttcacc aacactgatg
    17581 atcacaatat ctgcttcttt tgcaacagcc actgcttctg caaaacctac tgttgatgaa
    17641 tcattgatat tacaaccctt tgcatatact atttctgaag cggcactaag tttccgctga
    17701 acaccttccc ataaagaaga aattcttgcc gtatcatccg gccagtcata actccagaaa
    17761 ccattattat cagcttttgc tttggcaaac ggaccaatca gggcaatctt ttttgtttgt
    17821 ttactcaatg gaagtaattg cttttcgttt ttcagcaaca caatactttt cctggaaata
    17881 tcccttgcca cttttatatg ctccgcatta ttccattgcg ccttttctct ttcttcatta
    17941 ctgtatttat aggggtcatc aaatagcccc atttcaaatt tctttttcag gatacgccgc
    18001 accgcatcat caatcacatt catttttact ttcccctcct ttaccagtgc agccaggtgt
    18061 ttgatataac tccggctttc catatccata tcgctgccgg cattgactgc ctgcatggcg
    18121 gcttcataat tatctttaga aaacccatga ttgatcattt cacccacact accccagtcg
    18181 ctcaccacaa aacctttgaa tttccattcc cctttcaaaa tatctctttg caaatattta
    18241 ttaccagtgg ccggtatgcc attgaggtca ttgaatgaat tcatgaaggt ggctgcacct
    18301 gcttcagccg ctgctttgaa gggaggcaag tacacttcat gcaattgtcg caaactcata
    18361 tctacagaat tataatccct gccacccacc gcagcaccat aggctgcaaa atgtttagca
    18421 caggccataa gtccatctgt acttccaaac accttgccct ggaatccttt cactctcgct
    18481 gtagcgatca atgagccaag atatggatct tcaccggcac cttccatcac tcttccccat
    18541 cttggatcgc gggctatatc caccatcggg gcaaaagtcc agtgtacacc tacggctgct
    18601 gcttcagtgg cagcaatcct cgcagattgc tccattgcag gtatatccca gcttgctgct
    18661 tcccccaggg ggatgggaaa aatagttctg tatccatgta tcacatcctg cccgaataat
    18721 aaagggatct ttaatcttga ttgcatagcc agttgctgca actcttttgt tcttgccaca
    18781 cctgttacgt tcagcataga acccacttta ccgtctttta tctgcgataa taaattattg
    18841 tcattggtta aaggacctgt tgctgcccat ggtccattgt attggttcat ctgtcctgct
    18901 ttctcttcga gtgtcattaa ttttaagaca gaatccaccc tctggtcaat ggtttttttt
    18961 tgcgctgtac atacaataaa acttactagt gcaataaaca gaatgactga tcttttaaaa
    19021 actggattat acataacaga gtttttatac caaaatgaag attaaatata tttaagtttc
    19081 cggctagcca tgaataaaac tcactttgaa agtattatca ttgccaaccc gcctatatat
    19141 aagagcagca tgatattcag taaggatttt gttccctgtg gtgctttatc aaactccctc
    19201 catacataaa ttccccatag tgctgccacc accgttgcac cttgtcctaa tccatatgaa
    19261 accgccgggc cggctttacc cgatgctatt atactcaaag acatacctac gcaccatatt
    19321 actccaccta aaataccaat caaatgatcc tttgttatgc ctttaaaata gtcagtgaaa
    19381 gataccggcg taccaatgaa aggacgtttc atttgcagag aattaaaaag aaaactgctg
    19441 atcacaatac caatcgcaaa aattaccagg gccgtataag gagtcagctt cccggcatcc
    19501 ggtacattga aattggtaac cattgaaccg gcaacatac
//
LOCUS       DAORTI010000061        19059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2513_length_19059_cov_5.783835, whole genome shotgun sequence.
ACCESSION   DAORTI010000061 DAORTI010000000
VERSION     DAORTI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19059
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_19059_cov_5.783835"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1158)
                     /locus_tag="PLO70_09210"
     CDS             complement(208..1158)
                     /locus_tag="PLO70_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74685.1"
                     /translation="MKTAVLTNLFFVLVLTACSSAKNNIQTTTDDASPVTTIDQPFLA
                     PEQKPLSLMDLPQTQDGGFVLQPGFYEAEFKTYCLQPGTPDPREGDAYLQGPVTGYRK
                     SIVESILLNSRDQQDMEQKNIQLLLWSTVSGADYSKLSPAVKADATRLLTPKQIFELK
                     GGVMGLIKDVSRSTGVLNGNSDIKKLFETSIDSYEAYENIAVRREQSKVIKRGVKYDQ
                     WYKQQENYYVRYYPVSYKKVRIQVYMPENILDADNKLNNEYVVFDPTGQQAIPAFTNA
                     QRLGIGAPVIAVIREIIKVNKQTTPPKKVPEKKKNPKTGV"
     gene            complement(1431..2363)
                     /locus_tag="PLO70_09215"
     CDS             complement(1431..2363)
                     /locus_tag="PLO70_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Brp/Blh family beta-carotene 15,15'-dioxygenase"
                     /protein_id="HQZ74686.1"
                     /translation="MLRSLLLVSGVLILAGHWLFQPISANGQFIIFLTGILFLGIPHG
                     AADLLVANKTAETGKQKFNTIRFFAFYISRLIIFGALIYFLPAAGILLFLLFAAYHFG
                     ETDLHFFNTNSVIGKMLVCSYGLVILSVILLNNMDELQQLIQLSGVPVDRFPVLNWVA
                     QHHYLVLSVSLLLFFSNIFLYFILTKDTGIITEYFLVQFAVLVFLLFNLPLLTGFTFY
                     FIVWHSVLSLQNIISYLKAGDKYSHGTIIKQVILYSALAFIGILIVGSLGFMFISNQA
                     IIIYIFLGLAVLTAPHMQIMHDMYGHLRTHRKIG"
     gene            complement(2429..3166)
                     /locus_tag="PLO70_09220"
     CDS             complement(2429..3166)
                     /locus_tag="PLO70_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin-like"
                     /protein_id="HQZ74687.1"
                     /translation="MDLPLLDIQSISNDNYVAFTFFIGCMAMMAASAFFFFEMGNMEK
                     KWRTSILVSGLITFIAAVHYYYMRDYNLATGESPTFFRYVDWLLTVPLMCVEFYLITK
                     KAGAKISLLWKLIAASVFMLVTGYIGEAIYPTEKQSWIWGFVSSIGYFYIVYLIWFGD
                     VAKLAGQSGPAVAKAVRLLAWFVLVGWAIYPIGYIAGTEGGLFGAKILEGVSLDVVYN
                     IGDAINKIGFGLVIYALAQSDKELVKA"
     gene            3530..4852
                     /locus_tag="PLO70_09225"
     CDS             3530..4852
                     /locus_tag="PLO70_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74688.1"
                     /translation="MKNNLVTLAIALFSVLSSTAQQDGAALVKNAGKALTAYNIDPAG
                     NAAKLEEARNLIEKALKTPEAQALALAWITKGNIYNTRLQNDLTKRTINPNAGFTGDN
                     DALIAFEAYKNGYRLSEKSYEKSDCIKGIKEAQASLVNIGVSKYEEKKYEKSFSSFRA
                     AIESHDLLKANGQKSLLDEPGQLEDQLYITGLAALLANRCNDAMIFFNKLYKAGTDKP
                     EVYEGLYNCKLQMGDETGAGKILEEGRKKFPENTGLLFAEINFLLKNGRLTALTVRLE
                     EAIKKEPDNIGLYVTLGNVYDNLYQSAQKENDVAKATAYFDKAKKYYAEGLLKDPKNL
                     DANYSLGALYYNKAAIRTQQLNALPEDLSAEGIKKQTAIRNEIMGLFDQALPYFKKSE
                     SIAPNDINTLIALVEIFARKEDEAISQAMKKRLDIVKAGGKNQTSYFK"
     gene            complement(4862..5326)
                     /locus_tag="PLO70_09230"
     CDS             complement(4862..5326)
                     /locus_tag="PLO70_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQZ74689.1"
                     /translation="MKLFPLIAIIVSLSLDAQSQALPDFSFTSTKKENITPARLPANK
                     PIIVCYFDPFCEKCITQATKIRDKAAKFSNSTMVYVSWAELEDIEAFRKKYFTGMQNL
                     FMCKDTEYKFDSWFGYSEVPAIYIYNKARKKTAAFTSVVSAEEILAACTKTP"
     gene            5563..6807
                     /locus_tag="PLO70_09235"
     CDS             5563..6807
                     /locus_tag="PLO70_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74690.1"
                     /translation="MNNTYILLRNNSESTPLSLEDLKRIGLLPTDLLWVECQSVCWQK
                     PQEIPELKQLLTVADRPVIKPAIAEQPEKYAPPVENETVAGTHKNGVHVELPSNYKSL
                     TKKKEDTAPVKQESPALHTETKTPFTVNTNDSNHHLSGTDSQPVEGNKDPWMRNHAPK
                     DKTEMLESLLKLPPKKIALYAGLVAAGALMMLVIRGTGGHEQMRPQPVTQQTEIIKTT
                     PVEIPAEEQAADSTTTPESYAQQNIPIGQPTTNPAEEKIISAPQKNERTAKNTVSKKE
                     STPVTNETTEIKPVAVAEKKEVKKVSVENISSKLQLKANEYTVAAFGGIRNLKMTLQN
                     DSRYLLDKVSVELNYLNPEGKIVKTEKIDFNYVQPGAEATIAVNKTTRGVKLDYKITR
                     IELKETAANNSAAGENNIFSKN"
     gene            complement(6834..7613)
                     /locus_tag="PLO70_09240"
     CDS             complement(6834..7613)
                     /locus_tag="PLO70_09240"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQZ74691.1"
                     /translation="MFTLWQILIGLGLILLIILFVFRIGIVKEVVLLPENYRELLTSY
                     VKFYRQLDEESKEKFDKRVEHFLSAVKITGVNAVVEDLDRLLIASGAIIPVFTIPDWQ
                     YINLHEVLVYPGAFNADFDQAGSDRPMAGMVGSGAMQHVMIITKWQLRQGFINEQDTH
                     NTAIHEFVHLIDKMDGTMDGVPEIILERKYTAEWRNMMDSTIAQMKRHGSDIDMYGAT
                     NPAEFFAVISEYFFEQPDLLNANHPDLYAMLNRIYKTGNNG"
     gene            7821..8048
                     /locus_tag="PLO70_09245"
     CDS             7821..8048
                     /locus_tag="PLO70_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQZ74692.1"
                     /translation="MSKTKITVNNNGSLKVEGDFEVVDRNGGVYNLQGREIVSFCRCG
                     LSKNKPFCDGSHNGNFTHEAIAFDLPPKKTV"
     gene            8333..9460
                     /locus_tag="PLO70_09250"
     CDS             8333..9460
                     /locus_tag="PLO70_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74693.1"
                     /translation="MKNKSFYTTSVVSLFKKILIPFLIFLFSSPVHSQPATSASGWYF
                     TSYSFKEGSLRKESVLMGTTSKMYDTIYNKGDKGNMIITHNRYDVKTGKLVAGVSYKV
                     IWSDPPAVLQPEEKTSIDFESKTISSLSWKAPQRSVYINQGLYGIYYITPGGEKYINK
                     DISARLTTEKPIQKGTKGAKRLIQINFGNGFHAIYNYEWREGNTIQKPAQNVTANGWQ
                     LINYSFKDGSVKKNSVLMGTTSGLYDTISYKGDKGNIVITHNRYDVKTGKLLAGVSYK
                     VIWSDPPAVLQPEEKTSIDFELKTISSLTWKAPQQSIRADQGLYGVYYITPGGEKYIN
                     KDISARLTTEKSIQKGTKGAKRLIQINFGNGFSATYTYEWR"
     gene            9718..10974
                     /locus_tag="PLO70_09255"
     CDS             9718..10974
                     /locus_tag="PLO70_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ74694.1"
                     /translation="MIRRSIEIVVSSFKMALQELWKNKLRTFLSLFGITIGIFCIIGV
                     LATVNSLEQNIQNEVKTLGTNTIYVDKWDYSAGGGPDFPWWKFVNRPVPKYEETEQIK
                     ARTPSAKFVVFRNKVVDQVEYRGSALTNINIYGINEDFDNIQPVEIAHGRYLSEADFE
                     RGTNVVVIGNEIAEKLFGEAELSLEKHLDIRGKKMLIIGVVKKQGKQMIGGWDFDQSV
                     IVSYRLGRGIMNELKADPLIIVQGQDNINSKALKDDLTGTMRALRKLTPTKEDNFTLN
                     DINDFSAVISNAFVSLNIGGWAIAALSLIVGMFGVANIMFVSVRERTGQIGLKKAIGA
                     KRRIILTEFLLESAFLCIIGGLIGLTLVFLLTQLLTNVLNFPVFISSTYIALAIGICI
                     IVGVIAGFIPARQAAMMDPVEAMRSK"
     gene            11148..12188
                     /gene="tsaD"
                     /locus_tag="PLO70_09260"
     CDS             11148..12188
                     /gene="tsaD"
                     /locus_tag="PLO70_09260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQZ74695.1"
                     /translation="MIIILAIESSCDETSAAVCVDGKILSNFIANQTVHEKYGGVVPE
                     LASRAHMQHIVPVIDSALKTARCELSAVDAIAFTQSPGLIGSLLVGGQFAKSLALALD
                     KPLIAVHHMQAHVLANLIDDPKPSFPFLCLTVSGGHTQIVRCESPTNMKVIGETIDDA
                     AGEAFDKSAKLLGLPYPGGPLIDKYAKEGNPNRFKFPEPQIAGLDFSFSGLKTSILYF
                     LQNAGKSNLYKEEFLATEEERKQFIENNLPDICASIQHRIVTILLNKLKKASLETGIS
                     DICIAGGVSANSGLRKGIEEMGKKYNWNTFIPAFQYCTDNAGMIAITGYYKFLAGQFA
                     ELSVHSSARAEW"
     gene            12198..12932
                     /locus_tag="PLO70_09265"
     CDS             12198..12932
                     /locus_tag="PLO70_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQZ74696.1"
                     /translation="MSNTYFQFKQFTIHQDKSAMKVTTDGCLFGAWVVDKMTNEAFKN
                     NNCLDIGTGTGLLALMLAQKNPNLSISAIEIDIDSFEQASKNIADSPWSPGIKIVHGD
                     IKKFQSPEKFDFIISNPPFYKNELKGDNTKKNIAHHDEGLLLPELLTFIKNNLSPGGT
                     FYLLLPFKRNEEIKNLLLEHEFDIVHMTFVSQSPNHNFFRIMLSGKLKENKIPETVID
                     EIAIKDEKDNYTDAFIALLQDYYLHL"
     gene            complement(12952..14154)
                     /locus_tag="PLO70_09270"
     CDS             complement(12952..14154)
                     /locus_tag="PLO70_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ74697.1"
                     /translation="MIRIGLIREGKIPADNRVALTPAQCKWILKNSSDVKIIVQSSPD
                     RCFSDREYLSAGVELKEYLGECDLLFGIKEVPVAQLIPGKTYLFFSHTKKKQPQNQKL
                     LQAIIDKKITLIDYECLEHEDGQRIIGFGFFAGVVGAHNGMMAYGNRTGLFKLDRVYK
                     QRSFRELIHNYFGLRLPNVKIAITGSGRVAHGILEIMNLMEIHEMEPEDYLKRRFSYP
                     VYTQLKGAELYKHKETGKYNREHFHEHPEEYSCKFFKYTSQTDILMNGIYWDKTMPRL
                     FEKEDAKSENFIIQTIADITDDANGSVPINVGDQSIDDPVYGIDKSTLEKTAPYLPNS
                     IDIMAVGNLPNELPRDASRYFGEQLIKHVLEDLVGNGSEILERATMVKNGQLTNPFEY
                     LRDYAEGR"
     gene            14334..15146
                     /locus_tag="PLO70_09275"
     CDS             14334..15146
                     /locus_tag="PLO70_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74698.1"
                     /translation="MRNTKIILSAFSLIALSVSLLSCDKRGNVNDPAGPFTLSYGDSI
                     LYLRPSAGDYIVHPVSTRPGTYTGFPDGIEIDDNTGAINVSKSETGLRYRVTHTAPDG
                     TVTETKVVLSGITFKDHFYFLSNNDSIALPIYNADANRVLPLSGSVFDEGNLANTGGC
                     SVKTDNGKINLRETIRNGVFGFPPRNDAQETFEIRYRINDASGKTENRLKVLLYWYNT
                     INDVPQYVWDILNERTQQGVFLRNGALNNPTTTGRLAKEAKPRPPCVVIVDH"
     gene            15188..17980
                     /locus_tag="PLO70_09280"
     CDS             15188..17980
                     /locus_tag="PLO70_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQZ74699.1"
                     /translation="MQRFITFLLFTLFLVYCFSQKQAQDEPAYLPGYLAADRLYNEAV
                     LLPFQHNYNEEKESKLNLRSLHQFRQVLPAIIKDGNDSLAFFCHFKIASLFHYFDSLE
                     SAKNYYQQAIRLKTKLPGLADSFLFKPLLFTGSVYYSLNEFDSAFLFYKKAEQVSDQY
                     SSPLSEQQRLYNRLGAMHYETGNYKQAKNYFEKALSLLPLHDPFYKEFNANYKNNIAS
                     ALLKLERYNEARAIYLGLLPSGIYENEVKQNLGSISLNLGKPEEAITYFRDVNYNSSA
                     AILLFNKTGKAFLEAGKKDSADKYLQLALKENTKWNGSNKNVLHGLTFLYLAEKAAAE
                     NNIDASLGYYQEAIIQFHNDFNEMNIFKNPEEFSGLFSYINLFNTLTAKADLLERKYQ
                     QQKEAAVLESALAAYHAAFKLAGYVERTYDSDEARLFLGKIKHTVHSKPIDICILLFE
                     LTQKIHFLEEAYLFDQSNKASILLLNVQENELRKNADPENELLQRETLLKRAITRAAL
                     KAAQVKDSIVLQKLTADIRDNEIELSKVQEKIAADPAWKQKETTGKIPTVRELQGKLD
                     NNTALLSYHLSEKELLTLLITNKKLEYHKTIIDKTFFTRLEEFKSSLHNISPDQRYSG
                     EAVSSALYQTLIAPFYRTIKQGTRLVIIPDDELNYLPFEALQDENKKYLIEKFAVQYQ
                     FSTALLGDNDKTGSLSNTLALAPFASGSLKDSAGSAFTGLPASKEEISQLKGRVLTDE
                     NATKNIFLQLSNRYDIIHLATHASVNNDTPSHSYIAFYPTNNDYKLYAGEIADLKLDA
                     VQLVILSACETGAGQLVKGEGLMSLSRAFAYAGCPNIITSLWKAEDKTTSFITQRLHY
                     YLEKKYSKDKALQQAKLDFLRSDEIDSRLKTPDYWAHLLFIGNYEEDKGSSNWWYIAI
                     IIIAGVIVYKLINRKA"
     gene            complement(18027..18860)
                     /locus_tag="PLO70_09285"
     CDS             complement(18027..18860)
                     /locus_tag="PLO70_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HQZ74700.1"
                     /translation="MKNIITLAVLAILPVSIIFYSCQKDISGSTATDPDKPHGVTIYL
                     TDHQTPIFDSVFIDLQRLEIKLEDDTLPNSGWVNLTINPGVYNILRFRNGLDTLFATG
                     TLPNARVKKIKLVLGTQNSAMKDGQNFPLRVKDEDREVIANLDNSNFEITSSGQVLFW
                     IDFDAGNSIQVDNSGSGNNNGYRLKSHIKIFTRGSSGRIEGRVLPRAADAIVKAVIGT
                     DTATAIPDDDDGEFKIVGLNAGTYKVIIDGQNGYSDTTINNVIVRNREDTHLPVITLR
                     Q"
BASE COUNT         6124 a         3981 c         3674 g         5280 t
ORIGIN      
        1 tccggagggt tgctttaaaa aatggcatgt gggaaatatt aaagatgaaa aagcaaactg
       61 ccggagtaat gattacggcg gggtacttca attaaaccga atctactctt taatattggt
      121 ataactcaat aaagttccgg ggttgtctgt agtgtatctc cactaatcac ggtgtaatcc
      181 acctgcaatg ccccaggcaa caaataatca gactcctgtt ttcgggttct ttttcttctc
      241 cggtactttc ttcggaggag tggtttgttt atttacttta ataatttctc ttattacagc
      301 aatcactggt gcaccgatac caagccgctg tgcattggta aaagcaggga tggcctgctg
      361 accggtgggg tcaaagacta catactcatt attcaactta ttgtctgcat ccagtatatt
      421 ttcgggcata tacacctgta tccttacttt tttataactc accgggtaat agcgtacata
      481 ataattttcc tgctgcttat accattgatc gtatttcact cctctcttta tcactttgga
      541 ttgctccctg cgaacggcta tattttcata cgcctcatac gagtcaatac ttgtttcaaa
      601 taattttttt atatcgctgt tgccgtttaa tacaccggtt gaccgggaaa catcttttat
      661 caatcccatc accccgcctt tcagttcaaa gatctgttta ggcgtcagca gcctggttgc
      721 atcggcctta acggcaggag aaagcttact ataatcagcc ccactgaccg ttgaccagag
      781 cagcaattga atattctttt gttccatatc ctgctggtct cttgaattga gcaggataga
      841 ttcgacgata ctttttctat acccagtaac cggcccctgc aggtaagcat ccccttcccg
      901 gggatcggga gtgccgggtt gcaggcaata ggttttaaac tccgcctcgt aaaagccggg
      961 ttgcagaaca aatcctccgt cctgtgtctg tggcaaatcc atcaacgaca gcggtttttg
     1021 ctcaggtgcc aggaacggct gatcaatggt tgttactgga cttgcatcat cagtcgtagt
     1081 ttgaatattg ttctttgcag aagaacaggc ggtaagaacc aacacgaaaa ataagtttgt
     1141 aagtacagct gttttcatag cagggtattt agaggttacc aattgaagta cggatgcgaa
     1201 taatttgctg atacaaagtc tgaaattatc ttctcttata aaagtaaaac cggcgtaaag
     1261 tattgttaat agacccggcg gacgagtaat acaagtagtt ttcccattct tgctcttctg
     1321 cttccctgcc gggacatact ttttaatatt acttagtagt ccccaataat tgggacaagt
     1381 tatttcactg gccggccttt cctttgactg tctgcattgt taatctgtgt ctaaccaatt
     1441 tttcgatgtg tacgaagatg accatacatg tcatgcatga tctgcatatg cggagccgta
     1501 agaacagcca gtcccaaaaa aatataaata atgattgcct ggttactgat aaacataaat
     1561 cccagggacc cgacgataag tattccaata aaagcaaggg cactatataa aataacctgc
     1621 tttataattg taccatgaga atatttatcc cccgctttca ggtacgaaat gatattctgt
     1681 aacgataata cagaatgcca tacaataaaa taaaaagtaa atcctgttaa caaaggcaaa
     1741 ttaaaaagta aaaaaacaag gacagcaaac tgcactaaaa aatattcagt tattattcct
     1801 gtgtctttgg taagtataaa gtataaaaag atatttgaaa aaaagaggag cagcgatacc
     1861 gataaaacca ggtaatgatg ttgcgcaacc caattcaata ctggaaaccg gtcaacagga
     1921 acaccggaca gttggatcag ctgctgtaat tcgtccatat tattcagtaa aataactgat
     1981 agtataacaa ggccatagct gcacaccagc atcttgccta tcaccgaatt agtattgaaa
     2041 aaatgcagat cagtttctcc aaaatgatac gctgcaaaaa gtaaaaacaa tagtataccg
     2101 gcagcaggca aaaaataaat caatgcacca aaaatgatca acctcgaaat ataaaaagca
     2161 aagaaacgga tcgtattgaa tttttgttta cccgtttccg cagttttgtt ggccaccagc
     2221 agatcggctg ccccatgcgg gatacccaaa aaaagtatac cggttaaaaa aattataaat
     2281 tgtccattgg cagaaatagg ctggaaaagc caatgccctg ccaatattaa aacacctgat
     2341 actaataata aggagcggag catacctgtt ttattaaaga aaatacagta gtaccgatct
     2401 tgcgaccagg tactactgta cagaaagctt atgctttaac caattcctta tcagactgtg
     2461 ccaaagcata gatcaccaaa ccaaagccaa tcttattgat agcatcgccg atattatata
     2521 ctacatccaa tgacacccct tccaatattt tggcgccgaa taaaccacct tccgttccgg
     2581 ctatataacc gataggataa atagcccaac caactaatac aaaccaggct aacaatctta
     2641 ccgctttagc tacggccgga cctgactgac cagccagctt cgctacatca ccaaaccaaa
     2701 taaggtaaac gatgtagaaa taaccaatgc tgctgacaaa accccatatc cagctttgtt
     2761 tctcggtagg ataaatggct tcaccaatgt aaccggtaac gagcatgaaa acagaagcag
     2821 caatcagctt ccacaataaa ctgattttag cacctgcttt ttttgtgatc aggtaaaact
     2881 ccacacacat caatggcacg gttaataacc agtctacata acggaaaaat gtgggtgatt
     2941 cacctgtggc caggttataa tcccgcatat aataatagtg tacggccgca ataaaggtaa
     3001 taaggcctga aacaaggatt gaagtccgcc actttttctc catgtttccc atttcaaaaa
     3061 agaaaaatgc ggaagccgcc atcatcgcca tacagccaat aaagaaagta aaggcgacat
     3121 aattgtcatt agaaatggat tgaatatcca gtaatggtaa gtccatactt aaaattttaa
     3181 tttttaaaaa atgataatga atttctattc cgtataaact tcgaactgtt caagatcttg
     3241 tacgaaaggt taaacatatt ttgaaattaa aaggtttgca gattttcgat tttttttgaa
     3301 aattcagatt tgacagatgc tttcataaat aaaagttccc tgctgttaaa tctttacaat
     3361 ttaaaaaccg gaaagagtaa acaaaaaatc gtgttagttt atttttcaag tgaaaacatc
     3421 atgtcataaa tgatacaagg atcctgtgga agtaacttcc tctaaccaca gcttaaaatt
     3481 gtgcctgcat ttttcccttt ctttgtataa ttgtaaataa aaattgacga tgaaaaataa
     3541 tctggttacc ctggcaattg cccttttctc tgtactatcc tctacggccc agcaagatgg
     3601 cgctgcactt gtaaaaaatg caggcaaggc actcactgct tataatatcg atcctgcagg
     3661 caatgctgca aaattggaag aagccaggaa cctgattgaa aaggcactaa aaactccgga
     3721 agcacaggcc cttgcattgg catggatcac aaaaggtaat atatacaata cccgcctgca
     3781 aaatgattta accaaaagaa caattaatcc caacgctggc tttaccggtg ataatgatgc
     3841 cctcattgcg tttgaggcat acaaaaacgg gtacaggctt tcggagaaat cgtacgaaaa
     3901 atcagactgt ataaaaggta taaaggaagc ccaggcaagc ctggtcaata tcggggtgag
     3961 caaatatgaa gaaaagaaat acgaaaaatc attctcctcc tttcgtgctg caatagagag
     4021 tcatgacctg ttgaaggcaa acgggcaaaa aagcctgctc gatgaaccgg gccaactgga
     4081 agaccagttg tacattaccg gtttagctgc attgcttgca aatcgctgca acgatgccat
     4141 gatcttcttc aataaattgt acaaagcagg cacagataaa ccagaagtgt atgaaggcct
     4201 ttacaattgt aaactgcaga tgggcgatga aacaggtgcc gggaaaatac tcgaagaagg
     4261 tcgcaaaaaa ttcccggaaa atacaggcct cttgtttgct gaaatcaact ttttactgaa
     4321 aaacggccgg ttaactgcat taacagtccg gctggaagag gccatcaaaa aggaacctga
     4381 caatatcggt ctttatgtta cgcttggtaa tgtatatgac aacctgtatc aatccgctca
     4441 aaaagaaaat gatgtggcta aagccactgc ttactttgac aaagcaaaaa aatactatgc
     4501 ggaagggctg ctgaaagatc ccaaaaacct ggatgcgaac tattccctcg gtgcacttta
     4561 ttacaacaaa gctgctatcc gtacgcagca attgaatgca ctgccggaag atctatcagc
     4621 ggaaggaata aaaaaacaaa ctgctatccg caacgagatc atgggtttgt ttgaccaggc
     4681 attgccttat ttcaaaaaat cagaaagtat tgcccccaat gatatcaata cactgattgc
     4741 attggttgaa atttttgcac gcaaagaaga tgaagctatt tcacaggcaa tgaaaaaaag
     4801 attggacatc gttaaagccg gtggcaagaa tcaaacctcc tattttaaat aggcagaata
     4861 atcagggagt tttggtgcag gcagccagga tctcttcggc tgacacaact gaggtaaaag
     4921 cagccgtttt cttcctggcc ttattataaa tataaatagc aggcacttcg ctgtatccaa
     4981 accaactgtc aaatttatat tctgtatcct tgcacataaa caggttttgc atgccggtaa
     5041 aatacttttt tctaaaagct tcaatatcct ccaattctgc ccagctgaca tagaccattg
     5101 tactatttga aaattttgca gctttgtccc tgatcttcgt ggcttgtgtg atacactttt
     5161 cacaaaaagg gtcaaaataa caaacaataa tgggtttgtt tgcaggcagc cgggccggag
     5221 tgatattctc ctttttagtg ctggtaaatg aaaaatccgg taatgcctga gactgagcat
     5281 ccaaagaaag agaaacgatg atagcaatca acgggaataa tttcatacaa ttatttatag
     5341 tattaaatat attaaaatca agatatatct tacctctttt cttccgttta tccttttttg
     5401 gtcacttcaa tgaaagcatt acgtcttacc tgcacatata gcccccaaaa gtaacttcct
     5461 ccaaccacca ggtcaaagag taattctctt taaaaaagaa taacaatccc tttagaacta
     5521 acggcataga attttccgtt ctacagggca atctccacac atatgaacaa tacttatatc
     5581 ctgctgcgca ataacagcga aagcactccc cttagcctgg aagacctgaa acgtattggc
     5641 cttctcccta ccgacctgtt gtgggtggaa tgccagagtg tatgctggca aaaaccgcag
     5701 gagataccgg aactaaaaca actgctgacc gtggcagacc ggccggtaat taaacccgca
     5761 atagctgaac aaccggaaaa atatgctcct cctgtagaaa acgaaacagt ggctggtacc
     5821 cacaagaacg gtgtgcatgt tgaacttccg tcaaattaca agtcccttac taaaaaaaag
     5881 gaagacactg ccccggtaaa acaagaaagc cccgccttgc ataccgaaac aaaaaccccc
     5941 tttactgtaa atacaaatga tagcaatcac catctttccg gcaccgatag ccagcccgtc
     6001 gaaggcaaca aagacccctg gatgcggaac catgcaccga aagataaaac cgaaatgctg
     6061 gaatcgctgc tgaagttacc tcccaaaaaa atagccctct atgccgggtt ggtagcagcc
     6121 ggcgccctga tgatgctggt gataaggggc actggcggac atgaacaaat gagaccacaa
     6181 cccgtaaccc aacaaacgga aatcataaag acaactcccg ttgagattcc cgcagaagaa
     6241 caagcggcag atagtaccac cacaccggaa agttatgctc agcaaaacat tcccattgga
     6301 caaccaacaa caaacccggc tgaagaaaaa attatatccg caccccaaaa aaatgaaaga
     6361 acagcaaaga atacggtaag taaaaaagaa tccaccccgg taaccaatga aacgacagaa
     6421 attaaaccgg ttgcagttgc agaaaaaaag gaagttaaaa aagtatcggt tgaaaatatt
     6481 tcttcgaaac tacaattgaa agcgaatgaa tacacggtgg cggcctttgg tggcatccgc
     6541 aatcttaaaa tgaccttaca aaatgattcc cgttacctgc ttgataaagt atcggtggag
     6601 ttaaactacc tgaacccgga agggaaaata gtaaaaacag agaagatcga ttttaattat
     6661 gtacagccgg gagccgaagc cactatagcg gtgaataaaa ctacccgggg tgtaaaattg
     6721 gattataaga tcaccaggat cgaattaaaa gaaacagcgg ctaacaattc agctgcggga
     6781 gaaaacaata ttttttccaa gaattagaca aataaaagca cttacagggc cattcatcca
     6841 ttattccccg ttttatagat ccggttcagc attgcataca aatccggatg attggcgttt
     6901 agtaagtctg gctgctcaaa aaagtattct gaaataacag caaaaaattc agcaggatta
     6961 gtagctccgt acatatcaat atctgatcca tgccttttca tttgtgcaat tgttgaatcc
     7021 atcatattcc tccactcagc tgtgtatttc cgctctaaaa tgatttccgg aacaccatcc
     7081 atcgttccat ccattttatc aatcaaatgc acaaactcat gaatagcggt attgtgggta
     7141 tcctgttcat ttataaaacc ctgccgtaac tgccatttgg taatgatcat tacatgttgc
     7201 atagccccgc tacccaccat gcctgccata ggacgatctg aacctgcctg gtcaaaatcg
     7261 gcattgaatg caccggggta aaccaatact tcatgcaggt tgatgtattg ccagtcggga
     7321 attgtaaaaa cgggaataat agccccggat gcaattaaaa gccggtcaag atcttcgacc
     7381 accgcattta caccggttat tttcaccgcc gataaaaaat gctccactct tttgtcaaat
     7441 ttttcttttg actcctcatc cagttgccgg taaaatttta catagctggt cagcagctcc
     7501 ctgtaattct ccggcaacaa aacgacttct ttgacaatac ctatacgaaa gacaaatagg
     7561 attattaaaa ggatcagccc taaaccaatt aaaatctgcc agagagtgaa cataattaaa
     7621 tgaaggtaaa cagtatttat caactattga atgcctgtgc agggaacagt aattgagcta
     7681 tatttatagc gttaaaaaaa cttgtatgag tataccggga agttcgttac taagataaac
     7741 ttcgaaatct tttgcagtac gcatgaacgg ccacggaatt tctctacccg gtaaataaaa
     7801 aaatttatta aaaaagcaat atgtcaaaaa caaaaatcac cgttaacaat aacgggtcat
     7861 tgaaagtgga aggcgatttt gaagtagtgg acagaaacgg cggagtctat aatttacagg
     7921 gaagagagat cgtttctttc tgccgttgtg gtttgtcaaa aaataaaccc ttttgtgatg
     7981 gttcccataa cgggaatttt acacatgaag caatagcctt tgatctgcca ccgaagaaaa
     8041 ctgtttaatt cagtcaccca taggttctgc tgctgcagaa cctatggata cgaaaaatag
     8101 tatacttgta attctgctta tcattaattt tcccttcccg ttaagtatta tcctaaaata
     8161 atacgcccca gctactgttt tgctgcctat cagcaactat catcctgcat tcaaaaaagc
     8221 tggctcaatt ggtgagcaaa atcactatac acagtgagta atttcatcaa tacagaacat
     8281 atccctgact tactttacag taagcaaata ttttgtacag aaaactacgc atatgaaaaa
     8341 taaatcattc tatacaacat ctgttgtttc attgttcaaa aagatattga tacccttttt
     8401 gattttttta ttcagtagcc ctgtccattc tcaaccagct acctctgcat ccggctggta
     8461 ttttaccagc tattcattta aagaagggtc attaaggaag gaatccgtat tgatgggaac
     8521 tacttccaaa atgtatgaca ctatatacaa caaaggagat aaaggcaata tgataattac
     8581 ccataacagg tatgatgtaa aaacagggaa actggtggcc ggagtttcat ataaggtcat
     8641 ttggtcagat ccccctgcag tactgcaacc ggaagaaaaa acttctatcg attttgaatc
     8701 aaaaactatt tcttctttaa gctggaaagc accgcaaaga agtgtgtata taaaccaggg
     8761 actatatgga atatattata ttactcccgg tggtgaaaaa tatatcaata aagatatcag
     8821 tgcacgactc accacagaaa aaccaataca gaagggaacc aaaggagcaa aaagactgat
     8881 acaaataaac tttggcaatg gattccatgc tatttataac tatgaatgga gagaaggcaa
     8941 taccatccaa aaaccggcac aaaatgtaac tgccaatggc tggcagttaa tcaactattc
     9001 cttcaaggat ggttcggtaa agaagaattc agttttaatg ggaactacct caggattgta
     9061 tgatacgata agctacaaag gtgataaggg taatattgta attactcata acaggtatga
     9121 tgtaaagaca ggtaagctgc tggccggagt ttcgtacaag gtcatttggt cagatcctcc
     9181 tgccgtactg caaccggaag aaaaaacttc tatcgatttt gaactaaaaa ctatttcgtc
     9241 tttaacctgg aaagcaccgc aacaaagtat acgggcagac cagggattat atggagtata
     9301 ctatataact cccggcggcg aaaaatatat caataaagat atcagtgcac gactcaccac
     9361 agaaaaatca attcaaaagg gtaccaaagg tgcaaaaagg ttgatacaaa taaactttgg
     9421 gaatggattt tctgctactt acacttatga atggagataa aattaaattg aacaatacta
     9481 aggtctttcc attgccaatt taagaatatg ataaggtttg atcagttccg tgtaaaatag
     9541 tccttttaat ctatggcata gggctttaaa aatgttaaag caacagttta tgtcagagtt
     9601 tacagaactg aacttttcca gctgatgcac ccttgcacag gatactgtca aaaccaggtt
     9661 attcaccagt cataaatttg tttaccgtat catataaatc tccggtaatt ttgcggtatg
     9721 atccgccggt cgatagagat tgtagtcagc agttttaaga tggcattgca ggaactgtgg
     9781 aagaataagc tccgcacatt tctttcttta ttcggcatta cgatcggcat cttttgcatt
     9841 atcggtgtgt tagcaacagt caacagcctg gaacaaaata tccagaatga agtaaaaaca
     9901 ttgggcacca ataccattta tgtagacaaa tgggattaca gtgccggtgg tggtccggat
     9961 tttccctggt ggaaatttgt aaataggcct gtacccaaat atgaagaaac agagcagata
    10021 aaagcaagaa ccccttctgc caaatttgtt gtcttccgga ataaggtggt ggaccaggtt
    10081 gaataccggg gcagtgcttt gacaaacata aatatctacg gcatcaatga agattttgat
    10141 aatattcagc cggtagaaat tgcccatggc cgctacctct ccgaagctga ttttgaaaga
    10201 ggaaccaacg ttgttgtaat tgggaatgaa attgctgaaa aactatttgg agaagccgaa
    10261 ctctctcttg aaaaacacct ggatatccgt ggtaagaaaa tgctgatcat cggggtggtc
    10321 aaaaagcagg gaaaacaaat gatcggtggc tgggattttg atcagagtgt aatagtatca
    10381 taccggcttg gtcgtggtat catgaatgaa ttgaaagccg atccgctgat cattgtacaa
    10441 ggccaggata atataaacag caaagcgctg aaagatgacc tgactggtac catgcgggca
    10501 ctgcggaaac ttactcctac caaagaagat aatttcaccc tgaatgatat caacgatttc
    10561 agtgctgtca tcagtaatgc atttgtaagt ctgaacatag gcggatgggc cattgcagct
    10621 ttatcgctga ttgtgggcat gtttggtgtg gccaatatca tgtttgtttc tgttcgtgaa
    10681 agaaccgggc aaatagggct gaaaaaagca atcggtgcca aaagaaggat catccttacc
    10741 gagtttctgc tggagtccgc ttttctctgc attattggtg gcctgatagg attaacattg
    10801 gtttttttgc tgactcagtt acttacaaat gtgcttaatt tccctgtatt catttccagc
    10861 acttatatag cattagccat cggtatctgt ataattgtcg gggttatcgc cggctttatt
    10921 cctgcccggc aggcagccat gatggatcct gtagaagcta tgcggagtaa ataaatgata
    10981 gtgctgatgt gccgatttga caatttgata atgcggtgct gatagaataa ttatccttta
    11041 ctttgattat aaataattat ggaatcaatt caaaggggtc attaataaaa tcattgtaac
    11101 atctaagact taatcagcat attggcaaat cgtccaatta ccaaatcatg atcattatac
    11161 ttgcaataga atcttcctgt gatgaaacat ctgccgctgt atgtgtggat ggaaaaatat
    11221 tgtccaattt catagctaac cagactgttc atgaaaaata tggtggtgtg gtaccggagc
    11281 tggcttccag ggcccatatg cagcatatag ttcccgtcat tgattctgct ttaaagacag
    11341 cccgttgtga gttatctgct gttgatgcaa tagcgtttac acaatcaccg ggcctgattg
    11401 gttccttact ggtaggtggc cagtttgcaa aatcattggc actggcctta gataaacctt
    11461 tgatcgccgt acatcacatg caggctcatg tgctggcaaa cctgattgat gatcccaaac
    11521 cttcattccc ttttctttgc ctcaccgtta gtggaggaca tacacaaatt gtacggtgtg
    11581 aatcacccac caatatgaag gtaattggcg aaaccattga tgatgcggcc ggggaagctt
    11641 ttgataaatc tgccaaatta ctcggccttc cctatcccgg cgggccgctg attgataagt
    11701 acgcaaaaga aggtaatccc aaccgtttca aattcccgga accacaaata gcggggcttg
    11761 atttcagttt cagcggattg aaaacttcta tcctttattt tttacagaat gccggtaaat
    11821 ctaacctgta taaagaagag tttttagcca cggaagaaga gcgaaaacaa ttcatcgaaa
    11881 ataacctgcc tgatatttgt gcttctatac aacatagaat agttactata ctgctgaaca
    11941 aacttaaaaa agcatccctg gaaaccggca tcagcgatat atgtattgca ggaggtgttt
    12001 cggccaatag tggcctgaga aaaggaattg aagagatggg caaaaaatac aactggaata
    12061 cttttatacc tgcttttcaa tattgtaccg ataatgccgg catgatcgcc attaccggtt
    12121 attataaatt cctggctggc cagttcgccg agctgtctgt acattccagc gcaagagcag
    12181 aatggtaatt atttacgatg tcaaacactt atttccaatt caaacaattc accattcacc
    12241 aggacaaatc tgccatgaag gtgacaacag atggttgctt gttcggagca tgggtggtgg
    12301 acaagatgac aaacgaagca tttaaaaata ataattgcct cgatattggt accggcacag
    12361 gtttactggc acttatgctt gcacagaaaa atcccaacct atccataagt gctattgaaa
    12421 ttgacataga cagttttgaa caggcctcaa aaaatatagc tgattctccc tggtctcccg
    12481 ggataaaaat tgtgcacggc gatataaaaa agtttcagtc accggagaag tttgatttca
    12541 ttatcagtaa tccgcctttt tataaaaatg aattaaaggg agataatacg aagaagaata
    12601 tcgcccatca tgatgaaggg ctattgttgc ccgagctact gacgttcatt aaaaacaacc
    12661 taagcccggg aggaacattt tacctgctgc ttcctttcaa gagaaacgaa gaaattaaaa
    12721 atcttttact ggaacatgaa tttgatattg tgcatatgac gtttgtaagt caaagcccca
    12781 accacaattt tttccggata atgctttcag gaaaattaaa agagaataaa ataccagaga
    12841 cagtgattga tgaaatagcc attaaagatg aaaaagataa ctacaccgat gcttttatcg
    12901 ctttattaca ggattattac ctgcatttgt aaacaactat gggctaatga ctcatctgcc
    12961 ttccgcataa tcccttaaat attcaaatgg attggtcagt tgaccatttt taaccatggt
    13021 agctctttcc agtatttcag aaccattacc caccaggtct tccagcacat gtttgatcaa
    13081 ctgctcccca aaataacgac tggcatccct tggcaattca ttgggcagat ttcctactgc
    13141 catgatatca atagaattgg gtaaataggg agctgtcttt tccaatgttg atttatctat
    13201 tccatatacc gggtcatcga ttgactggtc tccaacattt attggaacgg agccattggc
    13261 gtcatctgtt atatccgcaa tggtttgaat gatgaaattc tctgacttcg catcttcttt
    13321 ttcaaacaac cggggcatgg ttttatccca ataaatgccg ttcatcagga tatctgtctg
    13381 ggaagtatac ttaaaaaact tacaggaata ttcttccggg tgttcatgaa aatgttcccg
    13441 gttgtactta cctgtttctt tgtgcttgta taattcggct ccctttaatt gcgtataaac
    13501 ggggtatgaa aaacggcgtt tcaggtaatc ttccggctcc atttcatgaa tttccatcag
    13561 gttcatgatt tccaggatac cgtgtgcaac acggcccgaa cctgtaatag caatctttac
    13621 attgggcaat cttagcccaa aataattatg aatcaattct ctgaaactgc gttgtttata
    13681 aaccctgtct aatttaaata atccggttct gttcccataa gccatcatgc cattatgggc
    13741 acctaccacc ccggcaaaaa aaccaaaccc gatgatgcgc tggccatctt catgctccag
    13801 gcattcgtaa tcgatcaggg ttattttttt atcaattatt gcttgtagca gtttctgatt
    13861 ttgtggttgt ttcttttttg tatgcgagaa aaagaggtag gtttttccgg gaatcaattg
    13921 tgccactggc acttctttga taccgaacag aagatcacat tcacccaggt attcttttaa
    13981 ttccacgccg gctgacaaat attcccggtc tgaaaaacaa cgatcgggtg atgactgtac
    14041 aatgatttta acatctgaag agttcttcaa tatccattta cactgtgccg gggtcaatgc
    14101 caccctgtta tctgcgggga tttttccttc ccgaataagt ccgatcctga tcatatagcc
    14161 gtttttcgct ttaaggtcag gcaaagttgc tgctaataat aatgccagac aaactgattt
    14221 aaatcaatct gcccggaata ttaaatgttg taactttaaa attactttat aaaatatttt
    14281 atacgcaggg ttacttttct tcgttttgag gttacacata aaaaagggtc ttcatgagga
    14341 ataccaaaat cattctttct gccttttcac tgatcgcttt atcagtatca ttgctatcct
    14401 gcgataagag gggtaatgtc aatgaccctg caggaccttt taccctcagt tatggtgatt
    14461 caatacttta cctgcgcccg tctgccggag attatattgt tcatcctgtt tctacccggc
    14521 cgggaaccta tacaggtttt ccggatggaa tagaaattga tgataatacc ggggccataa
    14581 atgtttcaaa aagtgaaacc ggtctgcgct accgtgttac gcatacggcc cctgatggca
    14641 ctgttacaga aacaaaggtt gtactatcag gcatcacctt taaagaccat ttttattttt
    14701 tatcgaataa cgactctatc gctttaccca tttacaatgc tgatgctaac agggtacttc
    14761 ccctgagcgg gtcagttttt gatgaaggta atcttgccaa tactggtggc tgttctgtaa
    14821 agaccgataa cggtaaaata aacctgcggg agaccatccg taatggagta ttcggcttcc
    14881 cgccaagaaa tgatgcacaa gaaaccttcg agatcaggta ccggatcaat gatgccagcg
    14941 gaaaaacaga gaacaggtta aaagtactcc tgtactggta taatacgatc aatgatgtac
    15001 cgcaatatgt atgggatatc ctcaatgaac ggactcaaca aggagttttt ctcagaaatg
    15061 gggctttgaa taatcccacc acaacgggac gtttggcaaa agaagccaag ccaaggcctc
    15121 cctgtgttgt aatagttgac cattaatgcc gcatgaacat taaatttgat aagcattcct
    15181 gcttatcatg caacgtttta ttactttttt actgttcact ttattcctgg tgtattgttt
    15241 ttcacaaaag caggcacagg atgaacctgc ttacctgccg ggatacctgg cagcagaccg
    15301 gctttacaat gaagcagtgc tgctgccatt ccaacataac tataatgaag aaaaggaaag
    15361 caaactcaat ctccggtctc ttcatcagtt caggcaggta ttgcctgcaa tcattaaaga
    15421 tggtaacgat tcactggctt tcttttgtca tttcaaaatc gcctccctgt ttcactattt
    15481 tgacagcctc gagtcagcaa aaaattatta tcagcaggct atccggttaa aaacaaaact
    15541 acccggttta gcggattcat tcctgtttaa accattatta tttacaggta gcgtttatta
    15601 cagtttaaat gaatttgatt ctgccttcct gttctataaa aaagcagaac aggtaagtga
    15661 ccagtatagc tccccacttt cagaacagca acggctttat aacaggcttg gtgccatgca
    15721 ttatgaaacg ggcaattata agcaagctaa aaattatttt gaaaaagccc tgtcgctgct
    15781 cccccttcac gacccttttt ataaagagtt caatgcaaac tataaaaaca atattgcatc
    15841 agcactgctt aaactggaaa gatataatga agccagggct atatacctgg gattgctgcc
    15901 atcgggtatt tatgaaaatg aagtcaaaca gaatcttggc agcatcagcc ttaacctggg
    15961 aaaaccggaa gaagcgatca cctatttcag agacgtaaac tataattcca gtgctgccat
    16021 cttactattt aataaaaccg ggaaagcatt tctcgaggct ggtaaaaaag attctgcaga
    16081 taaatacctt cagctggcat taaaagaaaa taccaaatgg aatggaagca ataagaatgt
    16141 actgcatgga cttacttttc tttacctggc agaaaaagca gctgcagaga acaacattga
    16201 tgcatcatta ggctactacc aggaggctat tatccagttt cataacgatt tcaatgagat
    16261 gaatattttc aagaacccgg aagaattcag cgggctattt tcatatatca accttttcaa
    16321 tacccttaca gccaaagcgg atcttttaga aagaaaatac cagcagcaaa aagaagcagc
    16381 agtactggag tctgccctgg ctgcatacca cgcagcgttt aaactggccg gttatgtaga
    16441 acgaacctac gactctgatg aggcccggtt attcctgggt aaaattaaac acactgttca
    16501 tagtaaaccc attgacatct gtattttatt gtttgaactt acacaaaaga tacatttcct
    16561 ggaagaggcc tatttatttg atcaatcgaa caaagcttcg atccttttac tgaatgtgca
    16621 ggaaaatgaa cttagaaaaa atgcagatcc tgaaaatgaa ttgctgcaac gggaaacatt
    16681 actcaagaga gctatcacca gagccgcact taaagcagca caggtgaagg acagtattgt
    16741 acttcaaaaa ttaacggccg atatcaggga caatgaaatc gaattaagca aggtgcagga
    16801 aaaaatagca gcggacccgg cctggaaaca aaaagaaacg acaggaaaaa tacctacggt
    16861 aagggaattg caaggaaaac tggataacaa tactgcattg ctgtcttatc atttatccga
    16921 aaaggaactc cttactttac ttattacgaa taaaaaatta gaataccata aaactataat
    16981 tgataaaact ttctttaccc gtctcgaaga gttcaaatct tccctgcata atatttctcc
    17041 cgatcaacga tatagcggtg aagcagtttc ctccgcattg tatcaaacac tcattgctcc
    17101 attttaccgg acgataaaac agggtacaag actggttatt atcccggatg atgaacttaa
    17161 ttacctgccc tttgaagcat tacaggatga aaacaaaaaa tatcttattg aaaaatttgc
    17221 agtacagtat caattttcta ctgcacttct tggcgataac gacaaaactg gcagcctgtc
    17281 aaataccctt gcccttgccc cttttgcatc cggcagtctt aaagattcag caggatcagc
    17341 attcactggt cttcctgcct ccaaagaaga aataagccag ttaaaaggcc gggtgttgac
    17401 tgatgaaaat gcaactaaaa atatatttct tcaactttca aacaggtatg atattattca
    17461 ccttgctaca catgccagtg taaacaatga cactccctct cattcttata ttgcctttta
    17521 tcccacaaac aatgactata aactctatgc cggggaaatt gctgacctga aactggatgc
    17581 tgttcaactg gtgatactca gtgcctgcga aaccggagcc ggtcaattgg taaagggaga
    17641 agggctgatg agcttgtcaa gagcatttgc ttatgccgga tgccccaata ttatcacttc
    17701 tctctggaaa gcagaggaca agactacttc atttatcact caacgactac attactattt
    17761 agaaaaaaaa tatagtaaag acaaagcgct gcaacaggca aaacttgatt ttttgagatc
    17821 cgatgagatt gattcccgct taaaaacacc ggattactgg gcacacttat tattcattgg
    17881 caactacgag gaagacaaag gttcatccaa ctggtggtat attgctatta tcataattgc
    17941 cggagtaatt gtctacaaac taataaatag aaaagcctga cgattttata tcgccaggcc
    18001 ctggttctcc ataacaggca aacatgctat tgacgtaatg ttataacagg taagtgagta
    18061 tcttcccggt ttctcacaat cacattatta attgtagtat cactgtatcc gttctgcccg
    18121 tcaataatta ctttgtacgt accggcattc aatcctacaa ttttaaattc tccgtcatcg
    18181 tcatcaggaa tggcagtggc agtatcagtg ccaatcacag cttttacaat ggcatcggca
    18241 gcccggggta acaccctgcc ttcaatcctt ccggaactgc ctcttgtaaa tatttttata
    18301 tgtgatttaa gcctgtaacc attattgtta cctgaaccac tgttatctac ctgtatggaa
    18361 ttacccgcat caaaatcaat ccagaaaagt acctgcccgc ttgaagtaat ttcaaaatta
    18421 ctgttatcca ggtttgcgat cacttccctg tcttcatctt taacccgcaa cggaaagttt
    18481 tgtccatctt tcattgcact gttttgagta cccaaaacca atttaatttt tttcaccctg
    18541 gcattaggta atgtaccggt ggcaaataaa gtatccagcc cgttacggaa tcggaggata
    18601 ttgtaaactc cggggttaat tgtaagatta acccatccac tgttaggcaa tgtatcatct
    18661 tcaagtttaa tttccagtcg ctgcaaatca ataaatacac tgtcaaaaat gggggtttga
    18721 tgatcagtca ggtaaattgt taccccatgt ggtttatcag gatcagttgc agtactaccc
    18781 gaaatatctt tctggcagga atagaaaata attgatactg gcaagatggc cagcactgct
    18841 aaagtgataa tgtttttcat aattgatgtt ttcatttttt ctgattgagt tacatctatt
    18901 ggtaaacact ggtaaataca ttgtaactat ttgaaataaa aaaacctctc ccggaatcgg
    18961 gagaggccac catccttaac aactcaaatt ttttaacgcc acctgagtag cttaacctgc
    19021 cggatcagtt tactggtaac ttttttatga taaagatga
//
LOCUS       DAORTI010000062        18968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2527_length_18968_cov_5.905726, whole genome shotgun sequence.
ACCESSION   DAORTI010000062 DAORTI010000000
VERSION     DAORTI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_18968_cov_5.905726"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLO70_09290"
     CDS             <1..952
                     /locus_tag="PLO70_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HQZ74701.1"
                     /translation="NQNADYLSMTLPYAAGSLISTVEDLWKWNQALHSYKLVTKESLQ
                     KAFTDYKLSNGKPTRYGYGWSFSTVQGSPSIEHGGGINGFLTDALYVPAEDIFVAIFS
                     NCECKSPGDLAPKLAALTMGKPYDRKEIAINAAAVKEYIGVYENEDGEQRIITADGMM
                     LTSQRSGGTKYSIKPYEKDKFFFENSLSTIVFLRNADGLIEKLQFKSRTEETEWSKTN
                     KPIPSGKTAINVDGVVLESYVGEYELAPNFILTVTHEGGKLMTQATGQEKFEVFAETK
                     TRFFLKVVDAQIEFYKDDTGKVTHLVLFQGGQKLEGKKIK"
     gene            complement(977..1582)
                     /locus_tag="PLO70_09295"
     CDS             complement(977..1582)
                     /locus_tag="PLO70_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HQZ74702.1"
                     /translation="MLRSYFIALCCFLFNTYCFSQYDWKLEKNKNGIKVYLSDVPGKN
                     FKAAKVDCTLTGTYAKLVGILTNVPNFPDWIYHNKSSKLIRRNNPLDFIYHTETSMPW
                     PMSNRDAIIHMRIKTDSLPRFLTITGTGEPGLLPKTSGKVRVSYFKANWKVTMPSAQT
                     INIIYTIELDPGGSVPAWIANMFADKGPYESFSNLADLLKK"
     gene            complement(1647..2660)
                     /locus_tag="PLO70_09300"
     CDS             complement(1647..2660)
                     /locus_tag="PLO70_09300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQZ74703.1"
                     /translation="MPGQKISLNHNKQLAAIFRQMSDCYKYMGPEQRFRALAYETVSK
                     TLSNMQQPIDILAPDIKKLDELKGVGDSIAEKIIEYLQTGKIRTFEKLKKQVPFPLLE
                     LMDIQGIGPATLRLLHDELNINSKEDLARAIEAGKLKKVKGFATKKIENLKKVLKLES
                     SKLRMPFKVAEKIGTEIQREIKKIPGVQQCLLAGSLRRKKESVGDIDVIITANHRSWK
                     KIIRMITMLPQVKKVLAAGQTKASIILNKNDVQVDIRIVHEDELGAALVYFTGSKEHN
                     IQLRTIGKQKGWKVNEYGVFDERTGKCLASATEEEIYSLFGLHFIPPEKRTGKDELTK
                     AKR"
     gene            2858..3409
                     /locus_tag="PLO70_09305"
     CDS             2858..3409
                     /locus_tag="PLO70_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQZ74704.1"
                     /translation="MRQSGAHSMFFVAIICPPSVDKKVLQYKRLMKEQFGCIVALKSP
                     AHITLIPPFWLEDEREKALVDTLQLFSSDIDTLKIHLNGFSHFGKRVLFVKVNENPAL
                     EEVKKQVENHFSKVFNDVFKKGDRTFHPHVTIANRDIKPSDFLTAWEYFSKKEFNEIF
                     PGITISLLKLSSGQWNIIGEKTW"
     gene            complement(3525..4823)
                     /locus_tag="PLO70_09310"
     CDS             complement(3525..4823)
                     /locus_tag="PLO70_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQZ74705.1"
                     /translation="MRTFFLTLFLLILGGFFSILQANDTLVLLSGQIPSERNNANRIL
                     ANSPPSVTASNSTLTLPKNSTVVTSTAVAANGIASTKWTKLKSPGQTLKTIVFLGSST
                     SIGFGLTSPQTNSFVGRTINYYNSQGLSNNLTWNVHNRALGGSNVFAAMPDGSPNTSN
                     QGPPDRNRNITWAIANGADIVVVNFPTNKYYIGSGGLSITEIMYAYRTIFNTAVAAGR
                     KCYITTTQPRTAAPDFDNLATWAFLKQIRDSILLQFGQYAIDFYSPIVAPGTSNIDPE
                     YNYGDNVHVNSAGHLQLFNRLVAANVFESFANCPALISTPSSNNTEISPIVQPSAHKF
                     QVSVIDTRGHAANAISTVTVNPCSGNTNYWIGIVSNEWGNPLNWSCGTVPVATTNVIV
                     SQGNPYYPTINSNITVRSITISNNVELTISPGIQLTVTGN"
     gene            5099..6109
                     /locus_tag="PLO70_09315"
     CDS             5099..6109
                     /locus_tag="PLO70_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQZ74706.1"
                     /translation="MDSLTHIALGACIGDAFAGRQLGKKAMFWGAMSQSLPDIDFVAS
                     IWSSTTEGLLAHRGFTHSILFALIMAPLLALAAERWHRPHNISLKKWILFFGVQILIH
                     LFIDGMNVYGVGWFEPFSHHRVSYNWIYVADPFYSVWLGISFVVLLLLRGNHPKRKWW
                     VEFGVGMSTIYLLYCGLNKIKIDNDVKDVFKKQQISYNKFFTTPTPLNNWLWYVVAEN
                     DSGFNIGYRSLFDRERKIDFRFVPKNEQLLKPVNNHEDLQKLIRFSKGYYAVQEWNDT
                     LVFNDLRFGQMIGWKNPEARFAFYYFLQHPDENKLVVQRGRFAGWDKDALRSLLKRIK
                     GN"
     gene            complement(6120..7298)
                     /locus_tag="PLO70_09320"
     CDS             complement(6120..7298)
                     /locus_tag="PLO70_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQZ74707.1"
                     /translation="MSRIKKLLRMGYSARNRVQLVKGGRDYFLLLKKLINKATHSIHL
                     QFYIFLDDETGKEIIEALKSAARRGVAVHLQLDAYASQGLHQKSVQELRNNGVSVKWF
                     EPLFKSKHFYFGRRLHHKVVVVDGLFSLVGGINICDRYNDMPSQPAWLDMAVYCEGEA
                     AYTTYHICRKMWGEKKTLASVSWKDADAFCETIPVGEQIEVRVRRNDWVTRKIDIWRS
                     YLEMFNKSEKQIIIMCSYFLPGLLYRRKLSQAIKKGVKVKVILAGISDIMIAKHAERY
                     LYDWILKNNIELYEYQETVLHAKVATYDNIWTTVGSYNINNISAYASLELNMEIRNNS
                     FALHTEKVLEDIINTSCKKITWENYSSSTHFFRRIWQRLSYGFINSVLKLFTFYFKQE
                     "
     gene            7427..7594
                     /locus_tag="PLO70_09325"
     CDS             7427..7594
                     /locus_tag="PLO70_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74708.1"
                     /translation="MNAIIPLEQVMNPVLIAWGLTAILLFVAMRFVKRPAAVLLLSVY
                     RFFIQLSSSRV"
     gene            7659..9323
                     /locus_tag="PLO70_09330"
     CDS             7659..9323
                     /locus_tag="PLO70_09330"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HQZ74709.1"
                     /translation="MNASVDIWRVLAGIAIFLLGVRFLEESLQRLAGRRFKLFLKKQT
                     SSKIKAIGGGALVTGVLQSSSVVSLMVLAFVGAGIITMKNALAVILGANLGTTITGWI
                     VALAGFNLNIETIALPVAGIAGLGFAIFNKQTKWFNWSRFLFGFSFLFVGLGYMKTGI
                     EGLVMSVDLQQYADAPLFVFFLIGFLITSLIQASSATMAITLSALFAGAVTLNDAIAI
                     ILGSEIGTTIKLFVASINGAASKRRVALGSFLFNAITVLLVFLFLFPLSRLVTEVIGI
                     KDNLIALVFFQSMVNILGIIIFYPFLDIFGRLLEKSFKTDQDETMFIHKADLRVPSAA
                     LVALENENRHFLFSVLDFTWGCFGERNRHLSAMNLHKDFPEKNLFEKYEYLKFLHGEI
                     HAYYIQLQKNVVDKEDIEKLDRLISSIRNTMYAAKSIKDAIPDLEQLKNSANDVKYNF
                     FCQTRDSVNEFCEKIYYLLKEGPALHFENITALYHSITGSYTNTLKQLYKDSTAGHLN
                     EIEITTLLNFNREIFTAFKSFVFGVKDSLFDKEQSKYFDELPGFIR"
     gene            9402..10733
                     /locus_tag="PLO70_09335"
     CDS             9402..10733
                     /locus_tag="PLO70_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQZ74710.1"
                     /translation="MKRVLFSVACLLIYAFINEPKFTHFAKSDRSALFSSNKKEDKLV
                     YPLLVDTFYIMNEGKLFWYKTDTSAISLREQLVAIIDTTGYYGLISKAYHVAELKSIL
                     QENFSDSLLLYEADRIYTDAAIAVFKDIYQGYNMKPWVGFDQVSAKYAMADDEFLLQL
                     LLNATSSVKLKNAVAMLEPADAAYASLKKELYRQKERNKKDSVASLLLSMNYYRWIHH
                     FKFDRFIVVNLAAARLQYYEDDSLLLQMKTVLGKSSTPTPRFAAWCDQVILYPYWYVP
                     KSIIFNEYMPILKKNPSWLDAHNMQVVDASGKVLDHHGLDWSSFHSGYFPYSIRQSTG
                     CDNALGVIKFNINTPYGVYLHDTNNKTAFFSASRYYSHGCIRLEDPIGLGSRLLGNKL
                     DTTYLQSCFKKQKPVYRQLDKPVPVFVVYMRAQTDTKGKVVYFRDVYRLMK"
     gene            10845..11327
                     /locus_tag="PLO70_09340"
     CDS             10845..11327
                     /locus_tag="PLO70_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74711.1"
                     /translation="MKKVKPPEKKAGVWLDQETAFIVHLIGDAEPVVERLKSGVESRI
                     RIPGEVKVSARFGQSFLDDKEKKQHRQRNQREKFFKSIIKMIHDVDYIYLFGPGKAKE
                     GLNNALEKDHSFKGKLVAIDTTDKLPRKQVLLKSINYFSDDAFKLYKKDLQKKQKAMS
                     "
     gene            11478..14402
                     /locus_tag="PLO70_09345"
     CDS             11478..14402
                     /locus_tag="PLO70_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQZ74712.1"
                     /translation="MENQLAQLENDFDGLLFFNGTAIHQAQLLAYSTDASVYQEKPLA
                     VAIPATIADIQKLIRFANQQKVTLIPRAAGTSLAGQVVGNGIVVDISKYFTRIIEVNA
                     KDKWVRVQPGVIRDDLNHYLKSFGLMFGPETSTASRAMIGGMLGNNSCGLHSIVWGSV
                     REHVREVKALLSDGSEVVFGTEDVLPKQETSSLKEKIYSDLYRLLSDGVNQDLIRNNF
                     PKSTVHRRNTGYALDSMLAMQPFKEDGETFNICKLLAGSEGTLAFITEIKLGLIDLPP
                     KETAVVCIHCHSIIESLRANIVALQHDPMASELVDKYIMDFTIHHPEYSKNRFFIEGD
                     PAALLMVEFMDDDAGRVQQKAKQLIEALEKEGLGYAYPVLYNEQTKFAWDVRKAGLGL
                     LRNLKGDTQPVNLIEDCAVSTDELPDYIADLQKVLEKHKVNASYYAHAGAGEVHVEPM
                     VNLKSEEGLKTFRNILSDTVELVKKYKGSLSGEHGDGRLRGEYISTVIGEETYRLFQQ
                     VKNIFDPHNIFNAGKITATPAMDTHLRVQQKPIKKNIKTTFDFSGEDGILRLAEKCSG
                     SGDCRKMPISGGTMCPSYMATRLEKDTTRARANLLRQFLSNEVDDQPFNHKEIKEIMD
                     LCLSCKGCKTECPSGVDVAKMKAEFLQQYYDKNGIPFRARLIANFSRQMKLVSKFPGL
                     FNWFYSVDLFRKIAHTLVGFHPERSMPKLAKQTLLNWFRKRNVDAATSKRKLYFFCDE
                     FTNYNDVEIGKKGILLLEALGYEVIIPKHIESGRTYLSKGLVKKAAGIANQNIQLLAD
                     LVMKEAPLIGIEPSAILTFRDEYIDLAAKENYQRAKELAANSYTIEEFLTMQLEAGMI
                     DRSLFTTASGKILIHGHCYQKSLSSQKFISSCLEIPANYKTSLIPSGCCGMAGGFGYE
                     KEHFTVSQQVGELVLFPAVRAAGVDDIIVASGTSCRHQVKDGTGKKAFHPVEVLYDAL
                     VK"
     gene            complement(14476..16677)
                     /locus_tag="PLO70_09350"
     CDS             complement(14476..16677)
                     /locus_tag="PLO70_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HQZ74713.1"
                     /translation="MIRLLLLQCCLFFSVDTMAATIIVKNLDELNAANKKAQPGDVII
                     LQNGEWKDATIRLNCHGTKEQPVVFKAQTAGKVMITGKSQLRLGGSFIVVDGLYFTNG
                     YAGKDPVINFRIDKDQLANNCRVTNTVINDFNNPKRMDENYWVAFYGKNNRLDHCSFK
                     DKKNIGVLLAVILDDERSRENFHAIDHNYFGRRPALASNGGEIIRVGVSQHCQFNSNT
                     QISNNFFEYCDGETEIVSIKSGSNTVNENIFKECQGSVVLRHGDNNLVTGNYFLGNDK
                     AGSGGVRIINKGQKVNNNIFFKCRGIDFRSPMAIMNGIPNSPAHRYVQVTDAEIINNS
                     FYECAPVTFCEGSDSERTLPPDNVIFSNNTFYNSKDSIIYKVYNDISGIKFGKNIVSS
                     KIKQVVINGFEKTIIPKQKPIVTRKGAESGPPHVNIIPLFEKETYSSSGAVWFSKKEA
                     IKEIKPLVFDCQTTDDIYKQLKREEPVIIRLTGKNYILTKPLVISKLVQFTGDKKNQV
                     KFTTENIFSVFLIAGKGNLTLNNLTINGEATKAIHLIASDSSGSSQHYNLSVRNCNIQ
                     NLSRKNGCENLFYACKYMVADSIVFRNNSFANNDINGIMMTEEKDNKGYYNAEKINIS
                     NNHFSNQGGTLLNIYRGGNDESTMGPLLILSNNTVSGCNTVNEEALITLTGTQSSLIG
                     RNSFINCNPGKVLLHFEDVVRAIHLFKNNTIKNSGKTIENKFVQQTNNVIQ"
     gene            complement(16685..17725)
                     /locus_tag="PLO70_09355"
     CDS             complement(16685..17725)
                     /locus_tag="PLO70_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQZ74714.1"
                     /translation="MNKPATTIKDIARQLNISISTVSRAMRNAPDVNADTKRAVMALS
                     EELNYQPNKLALSFKQKQTHNIGVIVPNLDYVLATMVKGIDEVALEAGYTVMVCQSNE
                     SYGREIVNTRRLLDSLVDGFIISVSSETKVFDHFKKIQENQMPMVVFDRVTPFLRAPG
                     VRIDNEDGGFQATEHLIEQGYKRIAILAGPKNLGISNQRLEGYLKALKKYKIKADEDI
                     IINCDFNQHYAYLATQELLNMKKRPDAIFTISDRMAIGAMLAIKEKKLKMPQDIGLVG
                     FNNEPVTSLVTPGISSVDQPAFELGKIAAKLFIETMHNNDDMSNVEEVLKPKLIIRES
                     SQRILPKIGRTK"
     gene            18059..18814
                     /locus_tag="PLO70_09360"
     CDS             18059..18814
                     /locus_tag="PLO70_09360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQZ74715.1"
                     /translation="MYSLKGKVAIVTGGARDIGRQASLKLAAAGARVCINYFGNKELA
                     EETLQLIKEAGGEAIAVQGDMTRAVEVKKVVDACVAAYGDTIHILVNVAGGLMGRKVL
                     ADLDEEFWDAVINVNLKSVYLVTRAVVPHMTAGGAIVNFSSQAARDGGGFGALAYATG
                     KGGVLTLTRGLAKELGPKGIRVNCVSPGMINTTFHNTFTKPEVRTNVAAATPLRREGE
                     AREVGDLVLYLASDASSFINGESVEINGGTYFA"
BASE COUNT         5439 a         3518 c         4029 g         5982 t
ORIGIN      
        1 caatcaaaat gctgattatc ttagtatgac acttccttat gcagccggtt cattgatctc
       61 cacggtagaa gatctttgga aatggaacca ggcgttacat tcctataaac tggttacgaa
      121 ggaatctttg caaaaggcat ttacagatta taaattgagt aatggtaagc caacccgtta
      181 tggatatggc tggtctttca gtacagtaca aggcagtcct tcaattgaac atggcggagg
      241 aattaacgga ttcctgaccg atgcgcttta cgttcctgct gaagatattt ttgttgccat
      301 cttttcaaat tgcgaatgta aatcacccgg ggacctggcc cctaagctgg ccgctttaac
      361 tatgggcaaa ccctatgacc ggaaagaaat tgccatcaat gccgctgctg taaaagaata
      421 tattggtgtg tatgaaaatg aagatggcga acaacggatc attactgcag atggaatgat
      481 gttgacatct caacgaagcg gtggcactaa gtacagcatc aagccatatg aaaaagacaa
      541 gttctttttt gagaattcgc tttcaactat tgtttttttg agaaatgcgg acgggctgat
      601 tgaaaagctt caatttaaga gccgcacaga ggaaactgaa tggagtaaaa caaataaacc
      661 gattccttct ggaaaaactg ctattaatgt ggatggagtt gtattagagt cctacgttgg
      721 tgaatatgaa ctggccccca attttatttt aaccgtgacg catgaaggag gtaagctgat
      781 gacacaggca actggacaag aaaaatttga agtatttgca gaaacaaaga cccggttctt
      841 tttaaaagtg gttgatgcgc agattgaatt ttataaggat gataccggca aggttactca
      901 ccttgtctta ttccagggag ggcagaagtt agaaggaaag aaaataaaat aacaggcttg
      961 ctttcaggtt gtgtttttat tttttgagca ggtcagccag gttgctgaaa ctttcatacg
     1021 gacctttatc ggcaaacatg tttgctatcc aggccggaac actgccgccg ggatcaagtt
     1081 ctattgtata aattatatta atagtttgtg ccgagggcat tgtcaccttc cagttagctt
     1141 tgaagtagga cacccgtact ttaccggaag ttttaggaag taacccgggt tcacctgttc
     1201 cggtgatggt aaggaacctg ggaaggctgt cagtttttat ccgcatatga atgatggcat
     1261 cccggttgga catcggccag ggcatcgaag tttctgtgtg ataaataaaa tcaagaggat
     1321 tattcctgcg aatcagttta ctgcttttat tatgatagat ccagtcaggg aagttgggta
     1381 cattagtaag tataccgacc agtttggcat aggttccggt cagtgtgcag tccactttag
     1441 cagctttaaa attctttccc ggtacatctg ataaatatac tttgatacca tttttattct
     1501 tctccaattt ccagtcgtat tgcgaaaaac aatatgtgtt gaacaggaag cagcaaagag
     1561 caataaagta actacgtaac atgattagat ttttaatgaa gccgctgtaa aatacaacga
     1621 tgttagttac cggcagctga tatttatcag cgcttcgctt tggtcaattc gtctttaccg
     1681 gttctttttt cgggaggtat aaaatgaagg ccaaataaag aataaatttc ttcttcagtt
     1741 gcagaagcaa ggcatttgcc tgttctttca tcaaacaccc cgtattcatt cactttccag
     1801 cctttttgtt tgccgatggt acggagttgt atattatgtt ccttgcttcc cgtaaaataa
     1861 accaaagcag cacccaattc atcttcatgt actatgcgga tatccacctg cacatcattc
     1921 ttgttaagga taatacttgc ttttgtctga cctgctgcca atactttttt aacctgtggg
     1981 agcattgtga tcatccggat gatttttttc catgatctat gattggcagt gatgatcaca
     2041 tcaatgtcac caacagattc tttcttacgc cggaggcttc cagccaggag gcattgctgt
     2101 acacctggta tttttttaat ttcacgctgg atctcagtac ctattttctc agctacctta
     2161 aatggcattc gcagtttgct gctttccagt ttcaatactt tctttaaatt ctcaattttt
     2221 ttggtagcaa agcctttcac cttcttcaac tttcccgctt cgatagccct ggcaagatct
     2281 tctttagaat taatgtttaa ttcatcatga agcagtcgaa gggtagccgg acctatacct
     2341 tggatgtcca ttaattcaag tagcgggaag ggtacttgtt tctttaattt ttcaaatgtt
     2401 ctgattttcc cggtttgcag gtattcaata attttttcag caatactgtc tccaacacct
     2461 ttaagttcat ctagtttttt tatatcagga gccagaatgt ctatcggctg ctgcatattg
     2521 cttaatgttt tggaaactgt ttcataggca agtgcccgga agcgttgttc aggtcccata
     2581 tatttataac aatccgacat ctgtcgaaaa atggctgcca gttgcttgtt atgattcagc
     2641 gatatttttt gaccaggcat tagtaaagca atattcaggc ttattataca tgaataaaaa
     2701 cttggagcaa caaaataacc cagtatgtca gtaaattgga tgattacggt cagtagtgaa
     2761 taaaactttc ctgagtacct ggagcggcaa taactttctt gcgcattaac tggtgtaaag
     2821 taaattgtag cagaattgaa tgaacaataa ctgaataatg agacaatctg gtgcacacag
     2881 catgtttttt gtggctataa tttgtccgcc ctccgtggat aaaaaggtat tgcagtataa
     2941 gcgcctgatg aaagagcagt ttgggtgtat agtagcctta aaatctccag ctcatatcac
     3001 attgattcct cctttctggc tcgaggatga aagggaaaag gcattagtgg atacattaca
     3061 attattttcc agtgacattg atacattgaa gatccactta aacgggtttt cacattttgg
     3121 gaaaagagta ttgtttgtga aagtgaatga gaaccctgct ttggaggaag taaagaaaca
     3181 ggtggaaaat catttcagta aggtatttaa cgatgttttc aaaaagggtg accggacttt
     3241 tcatccccat gttactattg ccaatcgtga tataaagccc tctgattttt taactgcatg
     3301 ggagtatttt tccaaaaagg agtttaatga aatttttccg ggcatcacta tttcattact
     3361 taagttaagt tcaggtcagt ggaatataat aggggagaaa acatggtaaa aaccagtttt
     3421 agaggttggt ttattaacag ttaagaaatt aagattattg aagacaataa ttgcctttac
     3481 gtcttggtat tcagatttcc ttttttaaag ttaaatagta aagatcagtt accagtaaca
     3541 gtaagttgga tgcccggact gattgtaagt tctacattat tactgatggt aatactcctt
     3601 acagtaatgt tggaatttat agtgggatag taaggattac cctggctgac gatcacattg
     3661 gtagtagcaa caggaacagt tccgcaactc cagtttaatg gattacccca ttcattactt
     3721 actattccaa tccaataatt agtattgcca ctgcatggat ttacagtaac cgtagaaata
     3781 gcattggcag catgtccccg tgtatcaata accgatacct ggaatttatg tgcagagggc
     3841 tgtacaattg gagaaatttc cgtgttattt gaacttggag tactaattaa ggccggacag
     3901 ttagcaaagc tttcaaaaac attagcagcc accaaccggt tgaataattg aaggtgacct
     3961 gcgctattaa catgaacatt atcaccgtaa ttgtactcag gatcaatatt agaagtaccc
     4021 ggcgcgacaa ttggggaata aaaatcaata gcgtactggc caaattgcag aagtatacta
     4081 tctcttatct gttttaagaa agcccaggtt gcaagattgt caaagtcggg ggctgctgtc
     4141 cttggttgtg tggtagtaat atagcatttt cttcccgctg ccaccgctgt gttgaagatg
     4201 gtacggtagg catacataat ttcagttata cttaaaccac cgctaccgat gtaatacttg
     4261 ttagttggga aattaacaac tacgatatct gctccgtttg ctattgccca tgttatattg
     4321 cgatttctat caggtggtcc ttgattggaa gtgttaggac taccatcggg catagctgca
     4381 aaaacattgg aacccccgag ggcacggtta tgaacattcc acgtcaggtt attactaagc
     4441 ccctggctat tataatagtt aatcgttctg cctacaaaag aattggtttg cggactagtt
     4501 aacccaaagc caattgaagt ggaggagccg agaaaaacaa ttgtttttaa agtctgaccg
     4561 ggtgatttaa gttttgtcca ttttgttgaa gcaattccat tcgctgctac cgctgtgctg
     4621 gttacaacag tactattttt cggtaaggta agagtgctgt ttgaagctgt aacagaagga
     4681 ggtgagttcg ccagtattcg attggcattg tttctttctg acgggatttg ccctgacaaa
     4741 agaaccaatg tgtcatttgc ctgcaaaatg ctaaaaaaac cacccagtat aagtaaaaat
     4801 aaggtcagaa aaaaggtccg caatgagtaa aattttggta tataaaagat ttaaatatct
     4861 gtaatactga taatatgtcc aatttttttt tgcaacctgt atataactat caattttgtt
     4921 ttaaaagtaa aatgtaattt gacgcggtat ctttaacgtt agatataggg tcatttgcta
     4981 atattaacgg aaaacgacca tggtaagtga attcttcagt taattaatat taataaaaaa
     5041 taaaatttgt aagttcgttt attatcattt tgtttgataa tcgtctcaat ttttttatat
     5101 ggattccctc actcatattg cattgggcgc ctgtattggc gatgctttcg ccggcaggca
     5161 attggggaaa aaggcaatgt tctggggggc catgtcgcag agtttacccg atattgattt
     5221 tgtcgcttct atttggtcaa gtacaacgga aggcctgctg gcacatcggg ggtttaccca
     5281 ttctatatta tttgccctga ttatggcacc actactggcc ctggcagccg aacggtggca
     5341 ccggccccat aatatctcac tcaaaaaatg gatactcttt tttggagtgc agattttaat
     5401 tcatttattc atagatggta tgaacgtata tggagtgggt tggtttgaac cttttagcca
     5461 tcaccgggta tcttacaatt ggatatatgt agctgatcct ttttattctg tctggttagg
     5521 tatttccttt gttgtgttgc tgctgttgcg gggaaatcat ccgaaaagaa agtggtgggt
     5581 agagtttggt gtaggtatga gtactattta tcttttatac tgcggattga ataagataaa
     5641 aatagataat gatgtgaagg atgtttttaa gaaacagcaa ataagttata ataaattttt
     5701 tactacacct actcccttaa ataactggct atggtatgtg gtagcagaaa atgattctgg
     5761 ttttaatatc ggctacaggt ctctgtttga tagggaacga aaaattgatt ttcgctttgt
     5821 gcccaaaaat gagcagctgc taaagccggt caataatcat gaggatcttc aaaaactgat
     5881 ccgtttttca aaaggctatt atgcagtaca ggaatggaat gatacgttgg tatttaatga
     5941 ccttcgcttt ggacagatga tcggctggaa aaacccggaa gcgaggtttg ccttttatta
     6001 tttcctgcaa catcctgatg aaaataaatt agtggtacaa aggggccgct ttgcgggatg
     6061 ggataaggat gcactcaggt ctttactgaa acggataaaa gggaattgat aattatagat
     6121 tactcttgtt tgaaataaaa ggtaaataat tttagtacac tgtttataaa tccatagctg
     6181 aggcgttgcc atattcttct gaaaaaatgg gtggatgaac tataattttc ccaggtaatt
     6241 tttttgcaac tagtattgat aatatcttcc agcacttttt ctgtgtgtaa ggcaaaggag
     6301 ttattcctga tttccatatt tagctcaagg ctggcgtagg cgctaatatt attgatatta
     6361 taagaaccca cagtagtcca tatgttatca taagtagcta ctttggcgtg taatacagtt
     6421 tcctggtatt cgtatagttc aatattgttc tttagtatcc agtcataaag atagcgttct
     6481 gcatgtttgg cgatcatgat atctgaaatg cccgccagta tcacttttac ttttactccc
     6541 ttttttattg cctgggatag ttttctccgg tacaataaac ccggaagaaa ataactgcac
     6601 ataataatga tctgtttctc tgacttatta aacatctcca ggtaacttct ccagatatct
     6661 atctttcttg taacccagtc gtttcttctt actcttactt cgatctgttc acctacagga
     6721 attgtttcgc agaatgcatc tgcatccttc cagctaacag aagccaatgt ttttttttct
     6781 ccccacattt ttctgcagat gtgataagtt gtataagcag cttcgccctc acaataaaca
     6841 gccatatcca gccaggccgg ctggcttggc atatcattat accggtcaca aatattgatg
     6901 ccaccgacta atgaaaaaag cccgtctaca accaccactt tatgatgtaa cctacggcca
     6961 aagtaaaaat gtttgctttt aaacaaaggc tcaaaccatt tgacagaaac accattattc
     7021 cttaattcct gcacactttt ctggtgcaat ccctgggaag catacgcatc cagctgcagg
     7081 tgaaccgcaa ccccccgccg ggcagccgat ttgagtgctt ctataatttc tttcccggtt
     7141 tcatcatcca ggaaaatata aaactgaaga tgaatcgaat gggttgcttt attaataagc
     7201 tttttcagca ggagaaaata atccctgcct cccttcacca gttgtacacg gttccgggca
     7261 gaatatccca ttcggagtaa ttttttaata cgactcatta ttgactggta gggtcatttc
     7321 aaatctattt ggcttccgaa attacagcta tgaggttaat caacaacctg cttgatccca
     7381 ctcatttata acaacaagga agtgatctat ctttaaaata caaagaatga atgctataat
     7441 acctttggaa caagtgatga acccggtatt aattgcatgg gggctgacag cgattctttt
     7501 gtttgtagca atgcgttttg ttaaaagacc agcagctgtg ttattgctga gtgtgtatag
     7561 attttttatt cagctcagtt ccagcagggt gtagcaacaa ttatccattt aatgtgccgg
     7621 ggtggtaaac tgccccggtt tttattaact taccactgat gaatgcatct gttgatattt
     7681 ggcgggtact ggcggggata gctatctttc tgctgggtgt caggttcctg gaagagtcgc
     7741 ttcagcgact ggctggccgg cggtttaaat tgtttttaaa aaagcaaaca tccagcaaaa
     7801 taaaagcaat tgggggagga gcgttagtaa ccggggtgtt gcaaagcagt tcggtagtaa
     7861 gcctgatggt attggcattt gtaggggcag gcatcatcac tatgaaaaat gccctggcag
     7921 taatattggg agccaacctg ggcactacaa tcactggctg gattgtggca ttggcaggtt
     7981 ttaatctgaa tattgagact attgcattgc cggtggcggg tattgccggt ttgggttttg
     8041 ccatcttcaa taagcaaacc aaatggttta actggagccg cttccttttt ggatttagtt
     8101 ttttgtttgt cgggctggga tatatgaaaa caggtattga gggtttggta atgagtgtgg
     8161 acctgcagca atatgcggat gcaccactgt ttgtattttt cttaatcgga ttcctcatta
     8221 cctcattgat ccaggcaagt tcagccacca tggcaataac gctaagtgct ctttttgccg
     8281 gtgccgttac gctaaatgat gcaatagcaa ttattctcgg ttctgaaatt ggtacaacca
     8341 ttaagttatt tgttgccagt ataaatggag cagcgtcaaa acgaagggtt gccctgggct
     8401 cttttttatt taatgccatt actgtattat tggttttcct gtttcttttc cccctgagca
     8461 gactggtgac agaggtgata gggataaagg ataatcttat cgcattggtt ttcttccaaa
     8521 gcatggtaaa tattttgggg atcattatct tttatccttt ccttgatatt tttggccgct
     8581 tactggagaa aagctttaaa acggatcagg atgaaactat gtttattcat aaggccgatc
     8641 tgcgtgtccc ttcggcagca ctggtggccc tggagaacga gaatcgccac tttttatttt
     8701 cggtacttga ttttacctgg ggctgttttg gcgaaagaaa caggcatctt tctgctatga
     8761 accttcataa agattttccg gaaaagaacc tgtttgaaaa gtatgagtac ttaaaatttc
     8821 ttcatggtga gattcatgct tattatatcc agttgcagaa aaatgtggtg gataaggaag
     8881 atattgaaaa gctggacagg ctgatttcct ctatccggaa tactatgtat gcagcgaaaa
     8941 gtataaaaga tgctatcccg gatttagaac aactgaagaa ttcagcgaat gatgtgaagt
     9001 acaacttttt ttgccaaacc cgtgattcag tcaatgagtt ttgtgaaaaa atctattacc
     9061 tcctgaaaga aggacctgcc ttgcattttg agaatattac tgcgctgtac cattctatta
     9121 caggtagtta taccaatacg ctgaaacagt tatataaaga cagtacggcc ggccacctga
     9181 acgaaataga gataaccaca ctgctgaatt tcaaccggga gatatttact gcatttaaat
     9241 cttttgtttt cggtgtcaaa gacagtcttt ttgataaaga gcagtcaaaa tattttgatg
     9301 aactacccgg ttttatccgc tgattttcaa ttgatatgta tcatattccg ggttgatgcg
     9361 gttcatttgt attaagcctg tattttgcca gatttgtagt gatgaaaagg gtactatttt
     9421 ccgttgcctg tttacttatt tatgctttta tcaatgagcc aaagtttaca cattttgcaa
     9481 aaagcgacag aagtgctttg ttttcatcaa ataaaaaaga ggataaacta gtttacccct
     9541 tgttggtaga tacgttttat ataatgaatg agggcaaatt gttctggtat aaaactgaca
     9601 cttccgctat ttcattacgg gaacaattag tggcaatcat tgataccacg ggttattacg
     9661 gacttatttc aaaagcttat catgttgctg aattgaaaag cattcttcag gaaaattttt
     9721 ctgacagtct gttattgtat gaagcagaca ggatatatac tgatgccgct attgccgttt
     9781 tcaaagatat ttaccaggga tataacatga aaccctgggt tggttttgac caggtttctg
     9841 ccaagtatgc aatggctgat gatgagtttt tgctgcaact acttttgaat gcgacatctt
     9901 cagttaagtt gaaaaatgct gtagctatgc tggaaccagc agatgcagcg tatgcctctc
     9961 tgaagaaaga gttataccgt caaaaagaaa gaaacaaaaa ggattctgtg gcctccctgc
    10021 tactatcaat gaattattac cggtggattc atcattttaa gtttgacagg tttattgtgg
    10081 ttaatttggc tgctgcccgg ttgcaatact atgaggatga tagcctgctg ctccaaatga
    10141 aaacagttct tggaaagtca tccactccta caccaagatt cgccgcctgg tgcgaccagg
    10201 tgatattata tccgtattgg tatgtaccaa aaagtatcat tttcaatgaa tacatgccaa
    10261 tcctgaaaaa gaatccttcc tggctggatg cacataatat gcaggtggta gatgcttcgg
    10321 gtaaagtact tgatcaccac gggttagact ggtcttcttt tcattccggt tatttcccat
    10381 atagtatccg gcaatctacc ggctgcgata atgcactggg cgttattaag tttaatatta
    10441 atacacctta tggggtttac ctgcatgata caaataataa aactgctttt ttttccgctt
    10501 cccgttatta cagtcatgga tgtatccgcc tggaggatcc aatcgggctc ggaagcaggc
    10561 ttctcgggaa taagctggat acaacttatt tacaatcctg ttttaaaaag caaaaacccg
    10621 tttaccggca attggataag ccggttcctg tttttgtagt atacatgcgg gcacaaactg
    10681 atactaaagg caaggttgtt tatttcaggg atgtgtatcg actcatgaag taagggttgc
    10741 cagatgagta gtatcatctg tttatatgag gccaattctt tttatgaggt tgtaagtaat
    10801 agatatttgg ttagtgatta ttctgaatac ttaaatttaa taatatgaaa aaagttaagc
    10861 cgcctgaaaa aaaagccggg gtctggcttg atcaggaaac tgcatttatt gttcatttaa
    10921 tcggtgatgc agaaccagtg gtggaaagat taaaatccgg tgtagaatcc aggatacgta
    10981 ttccgggaga agtaaaagtg tctgcccggt ttggccaatc ttttctggat gacaaggaaa
    11041 aaaaacagca tcgtcaaaga aatcagcggg aaaagttttt caaaagcatc attaaaatga
    11101 ttcatgatgt tgattatatc tacctttttg gtccgggaaa agcgaaggaa gggttgaata
    11161 atgcgcttga aaaagaccac agttttaaag gtaaattagt tgccattgat actactgata
    11221 agctaccccg taagcaggtg cttctgaaat ccataaacta tttcagcgat gatgctttta
    11281 aattgtacaa gaaagatctg caaaagaaac aaaaggccat gagctgacaa agaaaagacc
    11341 ggctttatac cggtcttttc tttgtctatt agtaacaatt attaccccag tgaaattatc
    11401 tttttttact ataataattg ttcgacattc attaaataaa gcagttaaat ttggctatcc
    11461 attaatgcat ttgaaatatg gaaaatcagt tagctcagtt ggaaaatgat ttcgatgggc
    11521 ttttattttt taatggtacg gcaatacacc aagctcaatt attagcctac tcaactgatg
    11581 catccgttta ccaggaaaaa ccactggctg tagcaatacc tgctaccatt gctgatatac
    11641 aaaaactgat caggtttgcg aatcaacaga aggttacgtt gataccgagg gctgcgggga
    11701 cttcacttgc aggacaagtg gtgggaaacg gaattgttgt tgatatctcc aaatacttta
    11761 cccggataat agaagtaaat gcaaaagata aatgggttcg tgtgcagccc ggagtgatac
    11821 gggatgattt aaaccattat ttgaaatcat ttggtttgat gtttgggcct gagacatcca
    11881 ctgccagccg ggcaatgatc ggaggcatgc tgggtaataa ttcctgtggg ctgcatagta
    11941 ttgtatgggg ttcggtaaga gaacatgtac gggaagtaaa agccttgtta agtgacgggt
    12001 ccgaagtggt ttttggaact gaagatgtac tgccgaagca agaaacttct tctcttaaag
    12061 aaaaaatata cagcgacctg tatagactat tgtcggacgg ggtcaatcag gatcttatcc
    12121 ggaataattt tccaaaatca actgtacaca gaagaaatac aggttatgca ttagatagta
    12181 tgcttgccat gcaaccattt aaggaagatg gagaaacgtt taatatatgc aaactactgg
    12241 ctggatcaga aggaacactg gcttttatta ctgagatcaa actgggtctt atagacctgc
    12301 cgcccaaaga aacggcagtt gtttgtattc attgtcattc catcatcgaa tcgttaaggg
    12361 ctaatattgt agcactgcag catgatccga tggcatccga actggtggat aagtatatta
    12421 tggactttac tatccatcac ccggaataca gtaagaatcg tttctttatt gaaggtgacc
    12481 ctgctgcact tctaatggta gaatttatgg acgatgatgc aggtagggtg caacaaaaag
    12541 caaaacaatt gatagaggca ttagaaaaag aaggattggg ctatgcctat cctgttttat
    12601 ataatgagca aaccaaattt gcctgggatg tgaggaaagc cgggctgggt ctgttacgga
    12661 acctgaaagg agatacgcag cctgtcaacc tgatcgagga ttgtgctgtt agtacggatg
    12721 agttaccaga ttatatagcc gatctccaga aagtattgga aaagcataag gttaatgcat
    12781 cctattatgc acatgccggt gccggggaag tgcatgtgga acccatggtc aatctaaaat
    12841 ccgaagaggg tttgaaaacg ttcaggaata tcctgtcaga tactgttgaa ctggtaaaaa
    12901 aatacaaagg ctcattgtca ggtgaacatg gagatggaag attacggggt gaatatattt
    12961 caaccgttat cggggaagag acctatcggt tgtttcagca ggtaaaaaat atttttgatc
    13021 cccataatat ttttaatgcc ggaaagatca cagctacacc tgccatggat acccatttga
    13081 gggtacaaca aaaaccgatc aaaaaaaata tcaaaacaac atttgatttt tccggggagg
    13141 atggaatatt gagactggca gaaaaatgtt ccgggtcggg agattgccgg aaaatgccaa
    13201 tatccggggg aactatgtgc ccgagttata tggcaacgag actggagaaa gatactacca
    13261 gggcaagggc aaatttgctt cggcaatttt tgagcaacga agtggatgac cagccattca
    13321 atcacaaaga gatcaaggag attatggatc tgtgcctgag ttgtaagggc tgtaaaacag
    13381 aatgcccttc cggtgttgat gtggctaaaa tgaaagctga gttcctccaa cagtattatg
    13441 ataagaatgg tattcctttc cgtgccaggc tgattgcgaa tttcagcagg cagatgaaac
    13501 tggtttcaaa atttcccggt ctttttaatt ggttttatag cgttgattta ttccggaaaa
    13561 ttgcccacac tctcgttggt tttcatcctg agaggtccat gcccaaactg gcaaaacaaa
    13621 ctttattaaa ctggtttcgt aaacgtaatg tggatgctgc cacttccaaa aggaaattgt
    13681 atttcttttg cgatgaattt accaattaca atgatgtgga gattggtaag aaaggtattt
    13741 tattgctgga agcattgggt tatgaagtga tcattccaaa acatattgaa agtggccgga
    13801 cctatttatc aaaggggctg gttaaaaaag ctgctggcat agccaatcag aatattcagt
    13861 tattggctga tcttgtaatg aaagaagctc ctttgattgg gattgaacct tcagctatac
    13921 ttacattcag agatgagtat attgatctgg cggctaaaga aaattatcag agggcaaaag
    13981 aacttgctgc taattcatat acgattgaag agtttttaac catgcaattg gaagcgggta
    14041 tgattgaccg gtctctgttt accactgcgt cagggaagat attgatacac ggacattgtt
    14101 accagaaatc attatcctct caaaaattta ttagcagttg tcttgaaatc cctgctaatt
    14161 acaaaacaag tttaattcct tcaggctgct gtggcatggc tggtggattt ggttacgaaa
    14221 aagaacattt tactgtttca caacaggttg gagaattggt attgtttcct gccgtgcggg
    14281 ctgccggagt cgatgatata atagttgcat ccggaacgtc ctgcagacac caggtaaaag
    14341 atggaactgg gaaaaaagca tttcacccgg ttgaagtgtt atatgatgcg ttagtcaagt
    14401 gaggccaatg catttcaaaa ccttttagca ggtattataa ttcccggact aatcaggtgt
    14461 ttttttaatt gttgcctatt gaattacatt atttgtttgc tgaacaaatt tgttttcaat
    14521 agtttttccc gaatttttaa tggtattatt cttaaacaaa tgtattgccc ttacaacatc
    14581 ttcaaaatga agtaagacct taccgggatt gcagttgatg aaactatttc ttcctatcag
    14641 ggaactttgt gtgcctgtta atgttattaa agcttcctcg tttactgtat tgcatccgga
    14701 taccgtatta tttgatagaa taagtaaagg ccccatggtg ctttcatcat tgcccccgcg
    14761 gtaaatattc agcaatgttc cgccctggtt actgaaatga ttattactga tatttatctt
    14821 ttctgcatta taatatcctt tgttatcttt ttcttctgtc atcattattc cattaatatc
    14881 attgttggca aatgaattat tccggaatac gatgctgtct gctaccatat atttgcaggc
    14941 atagaacaaa ttctcacacc cgttttttct gcttaaattt tgaatgttgc aattccggac
    15001 tgagaggtta taatgctggc tgcttccgct gctgtcactg gcaattaaat gaatggcttt
    15061 tgtcgcttct ccgtttatag tgagattatt cagtgtaaga ttccctttgc ctgcaatcag
    15121 gaaaacactg aatatatttt cagttgtgaa tttcacctga ttttttttat ccccggtaaa
    15181 ttgtacaagt tttgatatga ccaatggctt ggtcaaaata taatttttcc cggtcaaacg
    15241 gattattacc ggttcttccc gtttcagttg tttgtatata tcatctgtag tctggcaatc
    15301 gaagacaagg ggctttatct ctttgatggc ctcttttttg gaaaaccata ctgccccact
    15361 ggaggagtaa gtttcttttt caaataaagg aattatgtta acatgagggg ggcctgattc
    15421 tgctcctttc cttgttacta ttggtttttg ttttggtatg attgtttttt caaaaccatt
    15481 gataacgacc tgtttaatct tgctgctaac tatatttttt ccgaatttaa tgccactgat
    15541 atcattataa accttgtaga ttatactatc ttttgaatta taaaaggtgt tgtttgagaa
    15601 aatcacatta tcaggaggta atgtacgttc tgaatcactg ccttcgcaaa atgtgacagg
    15661 tgcacattca taaaagctgt tgtttataat ctcggcatcg gttacctgta cataccggtg
    15721 ggccggagag ttggggatcc cgttcattat tgccatggga gaacgaaaat caatccctct
    15781 gcatttgaaa aatatgttat tatttacttt ttgtccttta ttgataatcc gaactcctcc
    15841 ggagcctgct ttatcattgc ctaaaaaata gttgccggtc accaggttgt tatcgccatg
    15901 acgcaacacc accgagccct ggcattcttt gaagatattc tcatttactg tattgctgcc
    15961 ggatttaatg gagacgatct ctgtttcacc atcacagtat tcaaaaaaat tattactgat
    16021 ctgtgtgttg gagttgaact ggcaatgctg cgacacaccc accctgatga tctcgccgcc
    16081 attagaagcc aatgccggcc tcctgccaaa gtagttatga tctatggcat gaaaattctc
    16141 ccggcttctt tcgtcatcca ggatcactgc cagcaataca cctatatttt tcttatcctt
    16201 aaagctgcaa tgatcaaggc ggttattttt tccgtaaaaa gctacccaat aattttcatc
    16261 catccgtttc ggattattga aatcattgat cacggtattg gttaccctgc aattattggc
    16321 taattgatct ttatctatcc tgaaattgat cacgggatct ttaccggcat agccattggt
    16381 aaagtataat ccatctacaa caataaagct gccacccaac ctgagctgtg atttaccagt
    16441 tatcatcact ttccctgccg tttgtgcttt gaaaacaacc ggttgttctt tggtgccatg
    16501 gcagttcagc ctgatagtcg catctttcca ttcgccattt tggagtataa taacatctcc
    16561 cggttgtgct tttttgttgg cagcgttaag ctcatccagg ttttttacaa taatagttgc
    16621 agccatggta tcaacactaa aaaacaaaca gcattgtagc aacagtaatc gtatcatata
    16681 tatattattt agtccggcca atctttggca aaattctttg cgaggattcg cggatgataa
    16741 gtttgggttt taatacttct tcgacattac tcatatcgtc attgttgtgc atcgtttcaa
    16801 tgaatagttt agctgctatc tttcctaatt cgaaagctgg ctgatccaca ctggaaattc
    16861 ccggggtgac aaggcttgtt accggctcat tattaaagcc aacaagacca atgtcctgtg
    16921 gcatttttaa ttttttttcc ttgattgcga gcatggcgcc tattgccatc cggtcactga
    16981 tagtgaaaat ggcatccggc ctctttttca tattcagtaa ttcctgtgtg gcgaggtagg
    17041 cataatgctg gttgaaatcg caattgatga tgatgtcttc gtcagccttt atcttatatt
    17101 ttttgagtgc tttaagatat ccttccagcc gctgattact tatcccaaga ttttttggac
    17161 cagccagaat cgcaatgcgt ttgtatccct gttctatcag gtgttctgtt gcctggaaac
    17221 cgccatcttc attatcaata cgaacaccgg gagctctcag gaaaggagta acacggtcaa
    17281 ataccaccat cggcatttgg ttctcctgta tctttttaaa atgatcgaat acctttgttt
    17341 cactggagac ggatataata aatccatcta ccaggctgtc cagtaatctt cgggtattta
    17401 caatttccct accgtatgat tcattactct ggcaaaccat cactgtataa ccagcttcaa
    17461 gtgcaacttc gtcaatgccc ttaaccattg tagccaagac ataatcgaga ttaggaacga
    17521 tgactcctat attgtgtgtt tgtttttgct tgaaacttaa agccagctta ttaggctggt
    17581 aattcaattc ctcggataga gccataacag ctcttttggt atctgcattt acatccggtg
    17641 cattccgcat ggcacgggaa accgtagaaa ttgaaatgtt caattgcctg gcaatgtctt
    17701 taatcgtggt agcaggttta ttcatttggc gttataaagt aaagctaaat caaactttgt
    17761 tacgaaaatc ccggtagcgt tgccggtaac gttctcggtg ttaaaaagtt atgatttgtc
    17821 taatagataa tataaaatgt tagtatatga ctattaaaaa cgccatttat tttgaaaaaa
    17881 tcaaacgggt cattttcggg gcaggttgaa attcaaccta atattacaat ggagagattg
    17941 ctgtatggaa taaatcttgt cattattcag gtgtgttttt tttgtttcgg gttatttatt
    18001 cagtacctcc actttctccg aagactttac ctcactgttg ttttaaaata tttttattat
    18061 gtattcatta aaaggaaaag ttgcgatcgt aactggcggc gcccgtgata ttggcagaca
    18121 agcctcgctt aaactggctg ctgccggggc ccgggtctgt atcaattatt ttggaaacaa
    18181 agaactggca gaagaaacac tacagcttat taaggaagcc ggtggcgaag caatagctgt
    18241 acaaggtgat atgaccaggg ctgttgaagt taagaaagta gtagatgctt gtgtggctgc
    18301 ttatggtgac actattcata tcctggtcaa tgttgccggg ggactcatgg gccgaaaagt
    18361 attggctgac ctggatgaag aattctggga tgctgtgatc aatgtaaatc ttaaatctgt
    18421 ttacctcgtt acccgggcag tagtgccaca tatgacagcc ggaggagcta ttgtcaactt
    18481 tagttcacag gctgccaggg atggcggtgg ttttggtgcg ctggcttacg ccaccggaaa
    18541 aggaggcgta ttaacattga ctagagggct tgcaaaagaa ctcggcccca aaggtatcag
    18601 ggttaattgt gtatcaccgg gtatgatcaa taccacattt cataacacat ttacgaaacc
    18661 tgaagtccgg accaatgttg cagcagccac accattgcgg agggaaggag aagcaagaga
    18721 agtgggagac ctggtactat atcttgccag tgatgcttct tcttttatca acggtgaaag
    18781 cgttgagatt aatgggggaa cctatttcgc ttaggagatt ttcgaacggt ttcattaacg
    18841 ggtaacaagt atttaatatg cgattgctca acacgatatg cctgctttta ttccccctga
    18901 tgctgttggc gcagcacccg gttacttttt ttaccagggc ggaagctgct gaagttaaaa
    18961 aaaatctt
//
LOCUS       DAORTI010000063        18720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2598_length_18720_cov_6.019930, whole genome shotgun sequence.
ACCESSION   DAORTI010000063 DAORTI010000000
VERSION     DAORTI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_18720_cov_6.019930"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLO70_09365"
     CDS             complement(<1..883)
                     /locus_tag="PLO70_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQZ74716.1"
                     /translation="MTISGGISVIGAGTMGNGIAHVFAQRGFKVTLIDISQEQLDKAV
                     ATIGKNLDRMVAKEIIRPELKEQTLGNITTNTSIADGVKHAELVVEAATENVDLKLKI
                     FQQADAAAPAGCILATNTSSISITKIAAITHRPELVIGMHFMNPVPVMKLVEIITNPA
                     NEKETTDTIVELSKTLGKVPCVVNDFPGFIANRILMPMINEAIYSLHEGVAGVTEIDT
                     IMKLGMAHPMGPLQLADLIGLDVCLSIMQVLYDGFGNSKYTPCPLLVDLVKEGKLGIK
                     TGEGFYVYAAGSKGLVVS"
     gene            complement(1154..1795)
                     /locus_tag="PLO70_09370"
     CDS             complement(1154..1795)
                     /locus_tag="PLO70_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ74717.1"
                     /translation="MKKIILFTGLLFLILPQTVKAQFETSRDSVVQLFGVIMTADSLV
                     GIPAVSIMVKGQNRGTISNNQGVFSIVVLKGDEVEFTHVSYKTKLVRIPKNLEGNQYS
                     VVQLMVADTIYLPATILKPRPTPEQFARDFVNVKVPDDEYEIARKNTSAAKRKILMRT
                     TPGDGGEATRIQMNRIANKATYQGQAPPQNIFNPAAWAEFIQAWKRGDFKNKN"
     gene            1896..2987
                     /locus_tag="PLO70_09375"
     CDS             1896..2987
                     /locus_tag="PLO70_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQZ74718.1"
                     /translation="MTKEKVLEALSNVQEPDLGKDLVTLNMVKDIEIEGNYVTFTVVL
                     TTPACPMKDMIKNACVNAIKLLVNKEATVNVKFTSNTTTKRTDAGAILPKVKNIIAVV
                     SGKGGVGKSTVAANLALALSEGGAKVGLMDADIYGPSVPIMFGVRGERPIMMDVEGKG
                     MIIPLEKFGIKLMSIGLLVDEKNAVVWRGPMASSAIKQFVTDVYWDELDYLVVDMPPG
                     TGDIHLTLMQTVPVTGAVIVTTPQDVALADAKKGIAMFGQAQMNVPIIGLVENMSYFV
                     PAELPDNKYYIFGKEGGKRLADEYDIPFLGQIPLVQSIREGGDQGVPIMMSDDMVTRK
                     AFEDFAAFVVRSISMRNANISAEKIAETV"
     assembly_gap    3080..3179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3235..3630
                     /locus_tag="PLO70_09380"
     CDS             3235..3630
                     /locus_tag="PLO70_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74719.1"
                     /translation="MATITKFEDLEIWKLAREQAKEIYKLTKEGSFAKDFDLKNQINA
                     AAGSAMDNIAEGFERFSNKEFSQFLIISKGSNGEVRSQLYRAFDRGHITEEVLKMRLD
                     FSVMLGNKVKSFLDYLQSSKYKSKPRKKD"
     gene            3666..4307
                     /locus_tag="PLO70_09385"
     CDS             3666..4307
                     /locus_tag="PLO70_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQZ74720.1"
                     /translation="MYNLPHFKEKNHQEVIQFMKDHPFIFLTGVDSENKPVATQVPVF
                     IDERDGKLYLSGHMMRNTDHHKAFEINPAILAVFNGPHVYVSATWYDNPHQASTWNYM
                     SVHAKGQISFGGREELVTALKRLTLHYENNNTASTTVFDNLPDEYREPLMKAIVPFEI
                     EVTAADNVFKLSQNRDEKSYDNIIAQLEKQDEAAKYIAGQMRARRSQLFKDKP"
     gene            4340..5254
                     /locus_tag="PLO70_09390"
     CDS             4340..5254
                     /locus_tag="PLO70_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HQZ74721.1"
                     /translation="MNRNRVFKYALLVICFFAACQVSQKSLGTKSAMQTEFDKQGHRG
                     CRGLFPENTVPAMLYALGMGVTTLEMDVVITKDKKVILSHEPFFNHEISTKPDGKYVT
                     EAEEKSLNIYQLTYEEVLKYDVGLKPHPRFPGQEKIKAVKPLLGDVIDSVRKDMMTRR
                     RPFPYYNIETKTQPATDNSYHPAPEEFVELLMAVIKEKEIEDHVIIQSFDFRTLQYLH
                     QKYPDIKTAMLIEDYDKRTLEEQLQALGFTPTIYSPAYQLVNEALLNKCHTRNIKVIP
                     WTINTKEEIEKYKAMGVDGIISDYPDLF"
     gene            complement(5337..6770)
                     /locus_tag="PLO70_09395"
     CDS             complement(5337..6770)
                     /locus_tag="PLO70_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ74722.1"
                     /translation="MATITRFEDLEIWKLALQQCKDFDLLVEETALAKNFELRNQMDA
                     SSGSVMDCIAEGFDRSGNSEFKNMLIIAKGSNAEFRSQLYRCLNRKHITQERFDLLTD
                     KNILLGNKIMAFISYLQKTVYKGQRYKSGKALQPQTSNIKHQTSNLKLDIHTHIMPEH
                     MPNWTQKFGYGEFIHLEHRNCDACMMKGDKVFRMVEANSYQADARIKEMNETEVTIQV
                     LSTIPVLFNYWAKPEDGYETARFFNDHIAETVTKEPKRFIGLGTVPLQDIDLAIKEMD
                     RCVTELKMPGLEIGSNINGKNLSDKYFFPFYEAAEKAGCALFVHPWEMMGEDQMQKYW
                     LPWLVGMPAETARAICSMIFGGVFEQFPKLRVAFAHGGGSFPFTIGRIKHGFDVRPDL
                     VAVDNSVSPEEYIGKFWVDSLVHNKKSMEFLIDVMGEDKICLGSDYPFPLGEHHPGKL
                     IEKMKLGKKTEKKLLYKNAEDWLGVVE"
     gene            complement(6819..7343)
                     /locus_tag="PLO70_09400"
     CDS             complement(6819..7343)
                     /locus_tag="PLO70_09400"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HQZ74723.1"
                     /translation="MAVTAPFNLKKWIDEHRDLLKPPVGNQCVYKDASDFIVMVVGGP
                     NARKDYHFNETEELFYQLEGDITVKIVDDGVPKDIHIKEGEMFLLPARTPHSPQRGPN
                     TVGLVIEKVRKNEEDGFIWFCEKCGNMLYEEKLVVNDIVKQLPPVMEGFYSDEQKRTC
                     NKCGAVMLPPVKKV"
     gene            7409..8194
                     /locus_tag="PLO70_09405"
     CDS             7409..8194
                     /locus_tag="PLO70_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ74724.1"
                     /translation="MNLSLEEKKAIVCGSTQGIGLAIAEELALLGASCTLIARNEEAL
                     KEAVLKLDTQLRQQHNYLVADFSKPDELKKIIAAHVAKSPVHILINNTGGPAAGPITE
                     ATEEAFLNTFNQHLICSHILAKAVIPSMKKEGYGRIVNIISTSVKIPLKNLGVSNTIR
                     GAVASWSKTMANELGQFKITVNNILPGFTATQRLTTLIDSMAKKGNTVVDIVEKNMME
                     EVPMKRFAEASEVAAVAAFLASPAASYVNGVSIPVDGGRTGSI"
     gene            8282..9739
                     /locus_tag="PLO70_09410"
     CDS             8282..9739
                     /locus_tag="PLO70_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQZ74725.1"
                     /translation="MKLEIPYHLENYIGGHLIAPLSGNFIDNINPATGEVVGQIPDSH
                     KKDVDAAVQAAKLAFPDWSTSPVEERFKVLNRIAELIDAHLEELALAETNDNGKPLWL
                     SRQVDIPRASANFRFFATGIMHFSAESHNMEDKAVNYTLRQPIGIVGCISPWNLPLYL
                     FTWKIAPALAAGNCVIAKPSEVTPVTAFLLAKICKEAGLPGGVLNIIHGTGPGAGEAI
                     VSHPEVKAISFTGSTRAGERIAAIAAPKFKKLSLELGGKNPAIIFADCEWEKMMTETI
                     RSSFSNQGEICLCGSRILIEASVYDKFKTEFVERTKQLKVGDPLENESRQGAIVSKMH
                     FEKILRCIDTAKQEGGKILCGGNPVKLTGRCENGFFIAPTVMEGLGPNCQTNQEEIFG
                     PVVTLQSFKTEEEALQLANATSYGLAATIWTQDISKANRVAAKVESGIIWINCWLLRD
                     LRTPFGGMKNSGVGREGGWEALRFFTEAKNVCIQL"
     gene            9808..10224
                     /locus_tag="PLO70_09415"
     CDS             9808..10224
                     /locus_tag="PLO70_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HQZ74726.1"
                     /translation="MSEIIHTANASSPLGAYPHARKVGNLLFLSGIGPRNPKDNSIPG
                     LELDKDGNIVKYDIAAECHAVFANVKAVLEASGSSWDRIIDVTVFLTNMKKDFPVYNK
                     IYADYFTSVQACRTTVEVKSMPTPIAIELKVIATID"
     gene            10297..11571
                     /gene="kynU"
                     /locus_tag="PLO70_09420"
     CDS             10297..11571
                     /gene="kynU"
                     /locus_tag="PLO70_09420"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQZ74727.1"
                     /translation="MSFQNNLEFARDLDAKDVLKSFRQHFFIPQHEGKDCVYFTGNSL
                     GLQAKTTSEYVKQELDDWARLGVEGHFQAKNPWMPYHEIFPKQLSKIVGCKENEVVVM
                     NSLTVNLHLLMVSFYRPTKQRYKIICEAKAFPSDQYAFETQAKYHGFDPADAVIEVAP
                     REGEHILRTEDIISTIQQHNDSVAVVLFGGINYYTGQLFDMKAITAAAHAVGAYAGFD
                     LAHAAGNVELHLHDWQVDFACWCSYKYLNSSPGGIAGAYIHEKHAADTTLPRFAGWWG
                     YKKDTRFKMEKGFEAIPTAEGWQLSNAPVLLMAAHKASLDIFEAAGMDRLHTKRKRLA
                     AYLHFILHDINDRQPEKVIEIITPADEKERGCQVSMLMLQRGKEIFDVLTKQGVVADW
                     REPNVIRVAPVPLYNSFEDIYRFGEIIRSILK"
     gene            11649..12995
                     /locus_tag="PLO70_09425"
     CDS             11649..12995
                     /locus_tag="PLO70_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQZ74728.1"
                     /translation="MKKNITIIGAGLVGSLLSIYLSKREYNVRIYERRGDMRKEKMSA
                     GRSINLALSDRGLLALEKVGLAEEIKKISIPMHGRSIHNPDGSIAFQPYGKEGQYINS
                     VSRGTLNMKLMDLAEQHGVQIFFHEKCTTIDWDKDVLEFENTSGVSIPSSKADLVFAA
                     DGAFSAARLQHQLRHDKFNYQQYYIDCGYKELTIPATADGNFAMEVNALHIWPRKDYM
                     LIALPNLDKTFTCTLFFPFEGEASFDKLKTETDVKNFFEKTFPDAVPLMPDYINEFFT
                     NPTSSLVTIKCFPWIREDKFALIGDAAHAIVPFFGQGMNCGFEDCRVLDELIEKNGDD
                     WISILQQYQTLRKPDGDAIADLAINNFTEMRDRTADPVFLLQKKIEARLHEKYPEKWI
                     PAYSQVTFSPHIRYSEALKRGQMQEAIMQDVMKLENIGENWDNPDLHQQIIQQVQH"
     gene            13046..13849
                     /gene="pyrF"
                     /locus_tag="PLO70_09430"
     CDS             13046..13849
                     /gene="pyrF"
                     /locus_tag="PLO70_09430"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQZ74729.1"
                     /translation="MTRHQLVEQIREKRSYLCVGLDTDSTKIPQYLLAEPDPVFAFNK
                     AIIDATKDLCVSYKINTAFYEAMGVKGWEAMEKTVHYIGNEHFKIADAKRGDIGNTSD
                     QYAKAFFEAMPFDAITVAPYMGEDSVKPFLQYEGKWAIVLGLTSNKGANDFELQQAGD
                     GLLYEKVLQTVSAWGTPENLMFVVGATQADSFINIRKLTPNHFYLVPGVGAQGGSLKE
                     ISSKAMNRDCGILVNASRAIIYASGGEDFAEQAREVAKEYQEEMSGYLI"
     gene            complement(13809..14243)
                     /locus_tag="PLO70_09435"
     CDS             complement(13809..14243)
                     /locus_tag="PLO70_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74730.1"
                     /translation="MSYPVNEILRNFFSVVAGVTLALVIIIPSALFAAFLNNPDSAIP
                     TSQEILSTLLLISGIVAGCMLGGYTTVKVSTRKDIIHALITGIVLTILYALKNDFEFD
                     WRYPDTGIAYVGFIPIVMIGGIIGVRKKRTRLNNHSSLPGTP"
     gene            complement(14240..14671)
                     /locus_tag="PLO70_09440"
     CDS             complement(14240..14671)
                     /locus_tag="PLO70_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQZ74731.1"
                     /translation="MKKSLFPLVFLLCCTTVANSQKDSIKTKDEPRVKQFWMVILKTG
                     PQDKTITDSTQRSKLFAGHFSNMEKLYNDGLLKAAGPFGKNDFTWRGLFIMDCGTREE
                     AENHVKTDPTVAAGVFIYDIVPWWSEPSGSFTPGKPEKKEK"
     gene            14790..15974
                     /locus_tag="PLO70_09445"
     CDS             14790..15974
                     /locus_tag="PLO70_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQZ74732.1"
                     /translation="MAARTDLFNSPDYFLVDELLTEEHKLIRESVRSYVKKEISPIIE
                     DYAQNAEFPQQIVKQLGDLGCFGPTIPFEYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMFPIYQYGSEEQRKKYLPKLASGEWLGCFGLTEPNHGSDPGGMLTTIKDAG
                     DHVILNGAKMWISNAPFSQVAVVWAKNEAGEIQGLIVERGMEGFTTPTTHGKWSLRAS
                     ATGELVFDNVKVPKENIFPNVKGLKGPLGCLTKARYGIAWGVIGAAMDCYDTALRYSK
                     EREQFGRPIGGFQLQQKKLAEMITEITKAQLLNWRLGVLMSEGRATPQQVSMAKRNSC
                     EIATNICRDARQMLGGMGITGEYPVMRHMMNLESVITYEGTHDIHLLITGMDVTGLNA
                     FK"
     gene            16076..16582
                     /locus_tag="PLO70_09450"
     CDS             16076..16582
                     /locus_tag="PLO70_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQZ74733.1"
                     /translation="MKIFLIGFMGSGKTHWGRLLSEKLGIRFFDLDEQVTEHAGKTIP
                     EIFAAEGEEQFRLLEKDVLHIITESHDNFVMACGGGSPCYFNNIEYMNTAGTTVWINT
                     PQDTLHKRLVVEKDKRPLIKDLTDEQLRNFIQKKFSDRRIYYEQADIAIDEEPVQLEK
                     LIEKIFHV"
     gene            16575..16967
                     /locus_tag="PLO70_09455"
     CDS             16575..16967
                     /locus_tag="PLO70_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HQZ74734.1"
                     /translation="MYKNYLAIAAFFAGLAVALGAFGAHGLQKITSDETILHSYQTGV
                     QYQMYHALGLLAVAVLFEKFSHRWLRWVANCFITGMILFSGSLYLLTVLKIQGAETKW
                     VGPVTPIGGLFFIAGWLLLLVAVTRKND"
     gene            complement(16964..>18720)
                     /locus_tag="PLO70_09460"
     CDS             complement(16964..>18720)
                     /locus_tag="PLO70_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HQZ74735.1"
                     /translation="FTDHKADITQIIANEQFKPGVQQTGLIPIGMVSKKIILKFNICN
                     SADSVISVFFCPGFYFDKITMYRLEQEGLKIIPAVHPPISEGTGYRLISLPAHDTATI
                     IAELRAVKTYNNGIKPRLIGKDNLKLFLAKLHSNYNETNLITYVFCGLLLMMILFSIT
                     NFFQGRNREFLYYSGYAFFLGGLLFFQTIYHLHSSQIGFFLEGYLDFILQGVGIIFYM
                     LFMKKFLDTKNKHPFLSTLYNAGIILLCCSMFIYTFLFYFSDNYSSAFLVENLTKILL
                     LCMIIIFLFYSTRHWKDKLLRYLFWGNLFLFIFSFISQLGILMVQQFKSLPGILGSAL
                     IYYQVGLFLELVFFLAGLNYKNRKNIIVQTKERETLKAQNLLKEYEKELAVYKAQQEE
                     RQRISADMHDELGAGMTAIRLMSEIARNKMKENTPVEIEKISHSADDVLNKMNAIIWS
                     MNSGNDTLDNLISYIRSYSIEYFDNTPVQCKVTVPVTIPEKELTGDKRRNIFLSVKET
                     LNNVLKHAAASHVTIEIEANATLQIVIKDNGKGIDMEKLRQFGNGLKNIARRMESIGG
                     TYTIENNNGTITTLELPL"
BASE COUNT         5402 a         3652 c         4339 g         5227 t
ORIGIN      
        1 cactcactac cagcccctta cttcccgctg catacacata aaatccttct ccggttttta
       61 tacccagttt accttctttt accaggtcaa ccaacaaggg gcagggggtg tatttagaat
      121 tgccgaagcc atcgtacaat acctgcataa tagagagaca aacatccagc ccgatcaggt
      181 cggccagttg cagggggccc atggggtggg ccatacccag tttcattatg gtatcaatct
      241 ctgtaacccc ggccacgcct tcgtgcaggc tgtaaatggc ttcattgatc atcggcatta
      301 agattcggtt ggcaataaag ccgggaaaat cattcaccac acagggcact ttgcccagtg
      361 ttttgctgag ctccacaatc gtatccgtag tttctttttc attcgccggg ttggtaatga
      421 tctccaccag tttcatcacc ggcacggggt tcataaagtg cataccgatc accagttccg
      481 gccggtgggt aatagcggct attttagtaa tagaaataga agaggtattg gtagcgagta
      541 tacaaccggc cggcgccgcc gcatctgcct gctgaaatat tttcagtttc aggtccacat
      601 tctccgtagc cgcttccacc accagttctg catgcttcac gccgtctgct atcgaagtat
      661 tggtagtgat attgcccagt gtttgttcct tcagttccgg gcggataatt tctttggcca
      721 ccatgcggtc caggtttttg ccaatggtag caacggcttt gtctaattgt tcctgtgata
      781 tatcgatgag ggttacctta aagccccgct gtgcaaatac atgtgcgata ccgttaccca
      841 tggtgccggc gccgatgaca gagatgcccc cactgatagt catgattgtt tgatttttcc
      901 tttgcccctg aaggggagtt ttgaaagcct ccttccgggc aagagtgatt gaattagtta
      961 ggcagcaaaa atagggataa ggattcatcc ttgcagaata gatagggttg tggcgggcat
     1021 tcgaaaagac caggtataac aagcaggtta taaaaactaa agccagctta caattagaaa
     1081 atagatgctt taaaactttt tgataatcgt aatgaatctc agctctaagt tcccctttag
     1141 ggggacaggg ggcttaattt ttattcttaa agtctccccg tttccaggcc tgtataaatt
     1201 cggcccaggc agcaggattg aagatattct gcggaggggc ctggccctgg taggtggcct
     1261 tgttggcaat acggttcatt tgtatacggg tggcttcacc accatcaccg ggagtggtac
     1321 gcattaatat ttttcgtttg gcggcgctgg tgtttttgcg ggcaatctca tattcatcat
     1381 ccggaacttt cacattcaca aaatcacggg caaactgttc cggcgtgggc cgtggtttta
     1441 agatggtggc cggcaggtaa atagtatctg ctaccatcaa ctgcactacg ctgtattgat
     1501 tgccttcgag atttttgggg atcctgacca gtttggtttt atacgagacg tgggtaaatt
     1561 ccacttcatc cccttttaat accacgattg aaaaaacacc ctggttattg gagatggttc
     1621 ccctgttttg tccttttacc atgatgctga ctgcagggat gcccacgagg ctgtctgctg
     1681 tcatgatcac accgaataat tgaacgactg aatcacggga ggtttcaaac tgggctttga
     1741 cagtttgggg caggatcagg aacagtaaac cagtaaaaag tataattttc ttcactgcgt
     1801 caaaaataaa gacaagttgc tgatttcgta aaacaaattg ctgggtacaa tggttaactt
     1861 tgccgttctt attgatttta acaaatactt ggaaaatgac taaagaaaaa gtactggaag
     1921 cattgagcaa tgtgcaggag cctgacctgg gtaaagacct ggtgacattg aatatggtaa
     1981 aggatatcga gatcgaagga aactacgtca cctttaccgt ggtgctcaca acacctgcct
     2041 gcccgatgaa ggatatgata aaaaatgcat gtgtgaatgc gatcaaactg ctggtgaata
     2101 aagaagccac cgtaaatgtg aagttcactt ccaacactac taccaaacgt accgatgcag
     2161 gagcaatatt acccaaagta aaaaatatta tcgccgtagt aagtggaaaa ggtggtgtgg
     2221 gtaaatcaac cgtggcagcc aaccttgctt tggcattatc agaaggcgga gccaaggtag
     2281 gattgatgga tgctgatatt tacggaccca gtgtgcccat catgtttggt gttcggggag
     2341 aacggccgat catgatggat gttgaaggca agggcatgat cattccgctg gagaaatttg
     2401 gtatcaagct gatgagtatc gggttactgg tggatgaaaa aaatgctgtt gtatggagag
     2461 gcccgatggc gagcagtgcg atcaagcaat ttgttaccga tgtgtattgg gatgaactgg
     2521 attacctggt ggtggatatg cccccgggaa caggtgatat acatttgaca ttgatgcaaa
     2581 ctgttccggt tactggtgct gtgatcgtta ccaccccgca ggatgtggcg ctggccgatg
     2641 caaaaaaagg gattgcgatg ttcggacagg cacaaatgaa tgtgccgatc atcgggctgg
     2701 tggagaacat gagttatttt gttccggctg aactgcccga taataaatat tacatttttg
     2761 gaaaggaagg aggaaagaga ctggcggatg aatatgatat tcctttcctc ggccagatac
     2821 cattggtgca aagcatccgg gaaggcggtg accagggtgt gcccatcatg atgagtgatg
     2881 atatggtgac ccgcaaagca tttgaagact tcgctgcttt tgtggtaaga agtatttcca
     2941 tgcggaatgc gaatatcagt gcggagaaga ttgcggagac ggtgtaagtg ttcgaagttt
     3001 gaagtttgag gtttgatgtt tgaggtttga tgttcgaagt ttgaggtttg aggtttgaag
     3061 ttgtttcttc cctgaaattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3181 ttcgaagttt gaggtttgag gtttgaagtt gtttcttccc tgaaattctt tactatggct
     3241 acgatcacaa agtttgaaga tttggaaatc tggaagctgg cgagggaaca ggcaaaagag
     3301 atttataaat taactaagga aggaagtttt gcaaaagatt ttgatcttaa gaatcaaatt
     3361 aatgctgctg cgggttctgc aatggataat attgcggaag gtttcgagag attttccaat
     3421 aaagaatttt ctcagtttct tattatttca aagggttcga atggtgaagt aaggtctcaa
     3481 ttatacaggg catttgacag aggacatatt actgaggaag ttttaaagat gagattggac
     3541 ttttcggtaa tgcttggtaa taaagtcaaa tcctttttgg attacctgca atcttcaaaa
     3601 tataaaagca aaccacggaa gaaggattaa catccaacat ccaatttcca acatcaaact
     3661 atctcatgta caatttaccg catttcaaag aaaaaaatca tcaagaggtc attcagttca
     3721 tgaaggacca tccttttatt tttttaaccg gtgtggacag tgagaataaa ccggttgcga
     3781 cacaggtgcc tgtgttcatt gatgaaagag acggcaagct ctatttgtcg ggccacatga
     3841 tgcggaatac ggatcatcat aaagcatttg aaattaatcc tgctatattg gctgttttca
     3901 acgggccgca tgtatatgtc agcgccacct ggtatgataa tccgcaccag gccagtacct
     3961 ggaattacat gagtgtgcat gcaaaaggac agatcagttt tggaggcagg gaagaactgg
     4021 tcacagcgct gaagcgtctt accctgcatt acgaaaataa taatactgct tctacaactg
     4081 tttttgacaa tttgcccgat gaataccggg agccgttaat gaaagccatt gtgccttttg
     4141 aaatagaagt aactgctgct gataatgttt ttaaactgag ccagaaccgg gatgaaaaaa
     4201 gttacgataa tatcatagca cagttagaaa aacaggatga agccgcaaag tatattgccg
     4261 gccagatgcg ggcaagaaga tcacaattat ttaaagacaa accgtgaaaa gaagcccttc
     4321 ctatcgttat cattggatta tgaacagaaa cagagtcttt aaatatgctt tgctggtgat
     4381 ttgttttttt gctgcctgcc aggttagcca aaaatccctt ggtacaaaat ctgctatgca
     4441 aactgaattt gataaacaag gtcaccgtgg atgccgcgga ctttttcctg agaatacagt
     4501 gcctgccatg ctgtatgcat tgggcatggg tgtcaccaca ctggaaatgg atgtagtgat
     4561 caccaaagac aaaaaagtaa tattatcgca tgagcctttc tttaatcatg agatcagtac
     4621 caaacccgat gggaaatatg taaccgaagc ggaggagaaa agcctgaata tttaccagct
     4681 gacttatgag gaagtgctga aatatgatgt gggactgaaa ccgcatcccc ggtttcccgg
     4741 gcaggaaaaa ataaaagcgg tgaagccctt gctgggtgat gtgattgaca gtgtaagaaa
     4801 agatatgatg acccggagaa ggccatttcc gtattataat attgaaacaa aaacacagcc
     4861 ggctactgat aacagttatc atccggcacc ggaagagttt gtggagctgc tgatggcagt
     4921 gatcaaagaa aaggagatcg aagatcatgt gatcattcaa tcttttgatt tccgcaccct
     4981 gcaatacctg catcagaaat acccggacat taagacagcc atgctgatag aagattacga
     5041 taaacgtaca ctggaggagc agttgcaggc attaggattc accccaacca tttacagccc
     5101 ggcttatcaa ctggtgaatg aagcactgtt aaacaaatgt cacacccgga atatcaaagt
     5161 cattccgtgg acgattaata ccaaagaaga gattgaaaag tataaggcaa tgggagtgga
     5221 cggaattatt agcgattatc ctgatctgtt ttaagataag ttgataagca tatacggcga
     5281 cgggttaaca ggaggagtct gcagtctgca aacttatcaa cgggtcaact tgttaactat
     5341 tcaaccacac ccagccagtc ttctgcattt ttatagagta gtttcttctc cgtctttttc
     5401 cccaacttca ttttttctat cagtttaccc ggatgatgtt cgcctaaagg gaaggggtag
     5461 tcgcttccca ggcagatctt atcttcaccc atcacatcaa taagaaactc cattgacttc
     5521 ttgttatgca ccaggctatc cacccagaat tttccgatat attcttccgg ggagacagaa
     5581 ttgtcaacag ccaccaggtc cggccgcaca tcaaatccat gtttgatgcg gccaatggtg
     5641 aaagggaagg aacctccgcc atgtgcaaaa gccactctta gtttggggaa ttgttcaaac
     5701 actcctccga atatcatgga gcagatagct cttgccgttt ctgcaggcat gcccaccagc
     5761 cagggcagcc agtatttttg catctggtct tcgcccatca tttcccaggg atgaacaaac
     5821 aaagcacaac ctgctttttc agccgcttca tagaacggga aaaaatattt atcagataaa
     5881 ttttttccat tgatgttgga accgatctcc agccctggca ttttcaattc tgttacacag
     5941 cggtccattt cttttattgc caggtcaata tcctgcaggg gcacggtacc cagcccgata
     6001 aatcgtttcg gttcttttgt taccgtttct gcaatatggt cgttgaagaa tcttgctgtt
     6061 tcataaccat cctctggttt cgcccagtaa ttaaataaaa caggaatggt ggaaagcacc
     6121 tggatggtca cctctgtttc attcatctct ttgatccgtg catctgcctg gtacgaattt
     6181 gcttccacca tccgaaagac cttatcgcct ttcatcatac aggcatcaca gttgcggtgt
     6241 tccaaatgaa tgaattcgcc gtagccaaat ttttgtgtcc agtttggcat gtgttcgggc
     6301 atgatgtgag tatggatatc tagtttcaag tttgaggttt gatgtttgat gtttgaggtt
     6361 tgaggttgca gagctttacc acttttatag cgttgtcctt tataaacggt tttttgcagg
     6421 tacgatataa aagccattat tttatttcct aaaagaatat ttttatcggt gagcaggtca
     6481 aatctttcct gcgttatgtg ttttcggtta agacagcggt ataattgcga acgaaattct
     6541 gcattcgatc ccttcgcaat gatcagcatg tttttaaatt cactgtttcc tgatctgtca
     6601 aagccttcag caatacagtc cattacagaa cctgaggaag catccatctg gttgcgcagt
     6661 tcaaaatttt tagccagtgc tgtttcttca accaataagt caaagtcttt acattgctgc
     6721 aatgccagtt tccagatttc caaatcttca aaccttgtga tcgtggccat aataaagaat
     6781 ttcagtagag aacaacttca aacatcaaat gtcaaacctc aaactttctt aacaggtggc
     6841 agcattacag ccccgcactt gttacaggtt ctcttctgtt catcactata aaatccttcc
     6901 attaccggcg gtaattgttt tacgatatca ttgaccacta atttttcttc atagagcata
     6961 ttgccgcatt tttcgcagaa ccagataaag ccgtcctctt cgttctttct cactttttca
     7021 ataacgaggc caaccgtatt gggcccacgt tgcggggagt ggggagtgcg ggccggcagc
     7081 aggaacattt ctccttcttt aatatggatg tcctttggca ctccgtcatc tacaattttt
     7141 acagtgatat ctccttccag ctggtagaat aactcttctg tttcattgaa gtgataatcc
     7201 ttgcgggcat tgggtccgcc cactaccatc acaataaaat cgctggcgtc tttgtataca
     7261 cattgattgc ctaccggtgg tttcagcagg tcacgatgtt catcgatcca ttttttcagg
     7321 ttgaagggag cagtcacggc catatagcaa gtttttaatg atgtattgag gtggaaaaat
     7381 tacaactttt taccgtaggt ttgacgatat gaatctgtct ttggaagaaa aaaaagcgat
     7441 cgtttgcggc agtacccagg gcattggttt ggccattgca gaagaactgg cattgctggg
     7501 agccagttgt acattgatcg caagaaatga agaagcattg aaggaagcag ttttaaaact
     7561 cgatacgcaa ttgcggcagc aacataatta cctcgttgct gatttcagta aacccgatga
     7621 gcttaaaaaa ataattgctg cacatgttgc caaaagcccg gtgcatatcc ttataaataa
     7681 tacaggaggc ccggcggcag gaccgatcac cgaagccacc gaagaagcat ttctgaatac
     7741 attcaaccag cacctgatct gtagtcatat tttggcaaaa gcggtcatcc cttcgatgaa
     7801 aaaagaaggg tatggacgaa tcgtcaatat catttccaca tcagtgaaaa ttcccttgaa
     7861 aaatcttggt gtttcaaata cgatacgggg tgcagtggcc agctggtcaa aaacgatggc
     7921 taacgagttg gggcaattta aaataaccgt taataatatt ttacccggct ttactgctac
     7981 acaacggttg acgactctta ttgattcgat ggctaaaaaa ggaaatacag tagtggatat
     8041 tgttgaaaag aatatgatgg aagaagtgcc gatgaaacgt tttgccgaag cttcggaagt
     8101 ggcagccgtg gcagccttcc tggcatcgcc ggcggcttct tatgtcaatg gggtaagcat
     8161 accagtagac ggaggaagaa caggaagtat ttagagcaga acgcagatta tcatgatgat
     8221 tagatttgtt atgatttatc tgcgataatc atgaaaatca taataatcag cgttccattt
     8281 aatgaaactt gaaattccat atcacttaga aaattatatc ggtggacatt taattgctcc
     8341 gttaagcggc aattttattg ataatattaa tcctgccacg ggagaagtag ttgggcaaat
     8401 accggattcg cataagaaag atgtggatgc ggctgtacag gcggccaaac tggctttccc
     8461 cgactggagt acaagcccgg tggaggaaag atttaaggtc cttaaccgaa ttgcagaact
     8521 gattgatgcg catcttgaag aactggcgct ggctgaaacg aatgataatg gtaagccact
     8581 ctggttgagc aggcaggttg atataccaag agcatcagct aatttcaggt tctttgcaac
     8641 aggtatcatg cacttctctg cggagagtca taacatggaa gataaggcgg tcaattatac
     8701 tcttcgtcaa ccgatcggca ttgtaggttg tatctctccg tggaatctgc ctttgtattt
     8761 atttacctgg aaaattgcac ctgcactggc agctggaaat tgtgtgattg caaaaccatc
     8821 ggaggtgact ccagttactg catttttatt agctaaaatt tgtaaagaag ccgggttgcc
     8881 gggtggggta ttgaatatta ttcatggtac aggtcccggt gccggtgaag ccattgtttc
     8941 gcatcctgag gtaaaagcta tttcatttac cggcagcaca agggccggtg aaagaattgc
     9001 agcaatagca gcccctaagt ttaaaaaact ctccttagaa ttgggcggta aaaatcccgc
     9061 tatcatattt gcagattgtg agtgggaaaa aatgatgaca gagacgatcc gttcttcttt
     9121 ttccaaccag ggagaaattt gtttatgcgg tagtcgtatt ttgatcgagg catcggtgta
     9181 tgataaattt aagactgagt ttgtggagcg gacgaagcaa ttaaaagttg gtgatccgct
     9241 ggagaatgag tcaaggcagg gagctattgt aagcaaaatg cattttgaaa aaattttacg
     9301 ctgtattgat acggccaagc aggaaggcgg aaaaatttta tgtggcggta acccggttaa
     9361 actaaccggc agatgtgaga atggtttttt tatagcgcca acagttatgg agggattagg
     9421 cccaaactgc cagaccaacc aggaagaaat atttggtccg gtggtaacgc tgcagtcatt
     9481 taaaacagaa gaagaagcgt tgcaactggc caatgcaaca agttatggac tggcagctac
     9541 gatctggaca caggatattt caaaagccaa ccgggtagca gctaaagtgg agagtggtat
     9601 catctggatc aattgctggt tgctccgtga tctgcgaaca cctttcggag gaatgaagaa
     9661 tagtggagta gggagagagg gtggctggga agcgttgcgg ttttttactg aggcgaagaa
     9721 tgtttgtatt caattatgat aaatgttaag tgatgaatgt tgaatgaagg ttcatttaac
     9781 attcatcatt caaaattcaa catagtaatg tctgaaatta tacataccgc aaacgcatca
     9841 tccccgcttg gtgcctatcc gcatgcaaga aaagttggaa acttgttatt tctttccggc
     9901 attggtccga gaaacccaaa agacaattcc atcccgggat tggaattgga taaagacgga
     9961 aatattgtga agtatgatat tgcagccgaa tgtcatgctg tatttgccaa tgtaaaagca
    10021 gttttggagg ccagcggcag tagctgggac aggatcattg atgtgaccgt attcctgacc
    10081 aatatgaaaa aggattttcc ggtttataat aaaatttatg ctgactattt cacttccgta
    10141 caggcttgcc gcacaaccgt agaagtgaaa agtatgccaa cgccgattgc gattgaattg
    10201 aaagtgatag cgacaataga ttagttccgg gtttgacgtt tgatgttcga tgttaaagaa
    10261 cttcaaacct caaacttaga acttcaaaca attaatatga gttttcaaaa taatcttgag
    10321 tttgccaggg atcttgatgc aaaggatgta ttaaaatctt ttcgccagca ttttttcatt
    10381 ccacagcacg aagggaagga ttgtgtttac ttcacgggta actctctcgg tctgcaagcc
    10441 aaaacaactt cggagtatgt aaaacaggaa ttagatgact gggcaaggct gggtgtggaa
    10501 gggcatttcc aggcaaaaaa tccctggatg ccttatcatg aaatttttcc taagcaatta
    10561 tcaaagatcg ttggttgtaa agagaacgaa gtagtggtga tgaactcgtt gaccgtgaac
    10621 ctgcatttgc tgatggtgtc attttatcgg cccactaaac aacggtataa gatcatttgc
    10681 gaagccaaag cttttccttc cgaccagtat gcttttgaaa cacaggcaaa gtatcatggc
    10741 tttgatcccg ctgatgctgt gatagaagtt gcgccaagag agggagagca tatactacgg
    10801 acagaagata ttatttcaac aatacaacaa cataatgaca gtgtcgctgt tgttttattt
    10861 ggaggtatca attattacac cggccagttg tttgatatga aagccatcac tgctgctgca
    10921 catgcagtgg gtgcttatgc cgggtttgac ctggcacatg cagccggtaa cgtggaattg
    10981 cacctgcacg actggcaggt agattttgcc tgctggtgct cttacaaata cctgaacagt
    11041 agtcctggtg gtattgctgg tgcttatatt catgaaaagc atgctgctga tacaacattg
    11101 ccacgatttg ccggctggtg gggatataaa aaagataccc gtttcaaaat ggagaaaggt
    11161 tttgaggcga ttccaacagc ggaaggctgg caattaagca atgccccggt attattaatg
    11221 gcggcgcata aagcgtcgct ggatattttt gaagcagccg gaatggaccg cttgcatacc
    11281 aagcgtaaac ggttagccgc ttatttgcat tttatattac atgatattaa tgaccggcag
    11341 cctgaaaaag taattgagat cattacgccg gcggatgaaa aggaaagagg ttgccaggtg
    11401 tcgatgctca tgttgcaaag aggcaaagag atttttgatg tgttaacaaa acagggagta
    11461 gtagctgact ggcgggagcc gaatgtgata agagtagcac ccgtaccact gtacaattcg
    11521 tttgaagata tttacaggtt tggagaaatt atacgttcaa ttttaaaata gccacaacga
    11581 cacgaagtca caaagcagta taaaaaagtc tttgtgcacc ttcggtgcct ttgcgctttc
    11641 gggacaaaat gaaaaaaaat attacaatta tcggggcggg cctggtcggt tcattgcttt
    11701 ccatttacct ctccaaacgg gaatataatg taaggatcta tgaacggcgc ggggatatgc
    11761 ggaaagaaaa aatgagtgcc ggtcgttcga tcaacctggc actaagtgac aggggtttgc
    11821 ttgcactgga aaaagtgggg ttggcagaag agatcaaaaa gatatctatt cccatgcatg
    11881 gccggtctat tcataacccg gatggcagca ttgcttttca gccctatggc aaagaggggc
    11941 agtatatcaa ttcagtttcc aggggaacac tcaatatgaa actgatggac ctcgctgaac
    12001 agcacggcgt acaaattttc tttcatgaaa aatgtacaac gatagattgg gacaaggatg
    12061 tgctggaatt tgaaaatacg agtggcgtaa gtattccctc ttcaaaagca gatcttgttt
    12121 ttgctgcaga cggggctttt tcagccgcca ggttgcagca ccagttgcgc catgacaagt
    12181 tcaactacca gcaatattat attgactgcg ggtataaaga actgacgatt ccggcaacgg
    12241 ctgatggcaa ttttgctatg gaagtaaatg ccctgcatat ctggccacgc aaggattata
    12301 tgctgatcgc attacccaac cttgacaaga cctttacctg caccttattc tttccgtttg
    12361 aaggagaagc atcatttgat aaactgaaaa ctgaaacgga tgtaaagaat ttctttgaaa
    12421 aaacttttcc ggatgcggta ccgctgatgc cggattatat caatgagttt tttaccaatc
    12481 ctacttcctc actggttacc ataaaatgtt tcccctggat acgggaagat aaatttgcac
    12541 tgatcggtga tgcagcccat gccattgtac ctttcttcgg gcagggtatg aactgcggat
    12601 ttgaagactg ccgggtgctg gatgaactga ttgaaaagaa cggtgacgac tggatatcta
    12661 tcttgcaaca ataccaaaca ctgcgtaaac ctgatggaga tgcgattgca gatcttgcca
    12721 ttaataattt taccgagatg cgggacagga ccgccgaccc ggtattcctg cttcaaaaaa
    12781 agatagaagc aaggctgcat gaaaaatatc cggagaaatg gattccggct tattcacagg
    12841 ttacattcag tccgcatatc cgttacagcg aggccctgaa acgggggcag atgcaggaag
    12901 cgatcatgca ggatgtaatg aaattggaaa atattggaga aaactgggat aatcctgacc
    12961 tgcatcaaca gatcattcaa caagtacagc attgaaggat aacatccgaa cataaacttc
    13021 aaacctcaaa ctgtattttt gcaccatgac caggcatcaa cttgttgaac agatcaggga
    13081 aaaacgatct tacctctgcg ttggtttgga cactgatagt acaaagattc cccaatatct
    13141 tttggcggag ccagatcctg tatttgcttt taataaagca attattgatg cgacaaagga
    13201 tttgtgtgtg agctataaga tcaacactgc tttttatgag gcgatgggag tgaaaggctg
    13261 ggaggcgatg gagaagaccg ttcattatat tggtaatgaa catttcaaaa ttgcagatgc
    13321 caaaagggga gatattggca acacgtcgga ccaatatgcc aaagcctttt ttgaggcaat
    13381 gccttttgat gcgataaccg ttgccccgta catgggcgaa gacagcgtaa agcccttttt
    13441 acaatatgaa ggtaagtggg ctattgtttt gggattgacc tctaataaag gagccaatga
    13501 ttttgaattg caacaagccg gggatgggtt attgtatgag aaagtactac aaacagtgtc
    13561 tgcctggggc actcctgaaa atttaatgtt cgtggtgggt gctacacaag cagattcctt
    13621 tatcaatatc aggaaactaa ctcccaatca tttctatctt gttccgggtg ttggtgcaca
    13681 gggtggaagt ttgaaagaaa tttcttcaaa agcaatgaac agggattgcg gcattttggt
    13741 aaatgcaagc cgggccatta tttatgcttc cggaggggaa gattttgcgg agcaggcgag
    13801 agaagtggct aaggagtacc aggaagagat gagtggttat ttaatctagt acgttttttt
    13861 cttactccta ttattccgcc aatcatcaca attgggatga atccaacgta agcaatacct
    13921 gtgtccggat aacgccagtc aaattcaaaa tcatttttca atgcataaag gatagtcagc
    13981 actatgccgg taatcaatgc atgaataata tctttccttg ttgaaacctt tacggttgta
    14041 taacctccaa gcatgcaccc tgcgactatt cctgagatca acaaaagcgt tgaaaggatt
    14101 tcttgtgatg ttggaattgc tgaatccgga ttgttcaaaa aggctgcaaa taatgcacta
    14161 ggaataataa ttacaagtgc aagggttaca cctgccacca ccgaaaagaa attcctaagt
    14221 atctcattta ccgggtaact catttttctt ttttctccgg cttgccggga gtaaaactgc
    14281 cgctcggttc gctccaccag ggaacaatat cataaataaa aacaccggct gcaaccgttg
    14341 gatcggtttt tacatgattt tccgcttctt ctctggttcc acaatccata atgaacagtc
    14401 cccgccaggt aaaatcgttt ttaccaaaag gcccggctgc ttttaataag ccatcattgt
    14461 acagtttctc catattggaa aaatgcccgg caaataattt tgaccgttgt gttgaatcag
    14521 taattgtttt gtcctggggc ccggttttca atatcaccat ccagaattgc tttacccggg
    14581 gttcgtcttt tgtttttatg gaatcctttt ggctattggc taccgttgta cagcataaaa
    14641 ggaaaacaag gggaaaaaga gattttttca tgttgttgct ttacagcata aaaataaagg
    14701 attgttttca ctaagaatag tcgctgctac tgttttcata cctttgtccg ggaaaattta
    14761 aaacttaaaa tcaaaaatct caaataacga tggcagcccg tacagacctt tttaacagcc
    14821 cggattattt cttagtagat gaattattaa ctgaagaaca taagctcatc cgtgaatccg
    14881 tccgtagtta tgtaaagaaa gaaatttctc ccattataga agactatgct caaaatgcag
    14941 agtttcccca gcaaatagtt aagcagttgg gcgaccttgg ttgctttgga ccaaccattc
    15001 cttttgaata tggtggtggc ggattagatt atatcagcta tggattgatg atgcaggaac
    15061 tggaaagggg agacagcggt gttcgttcta ccgcatccgt gcagggatcc ctggtgatgt
    15121 tcccgatcta tcaatatgga agcgaagagc aacgtaaaaa atatttgccc aaactggcca
    15181 gtggggagtg gctcggttgt tttggattaa cagaacccaa tcatggcagc gaccccggcg
    15241 gcatgctcac tactattaaa gatgcaggtg accatgtaat actgaacggt gccaaaatgt
    15301 ggatcagtaa tgcacccttt tcacaagtgg ccgttgtatg ggcaaaaaat gaagccggcg
    15361 aaatacaggg tttaatagtg gaaagaggaa tggaaggttt caccacccca actacacatg
    15421 gtaaatggag tttaagggca tctgccacag gcgaactggt ctttgataat gtaaaagttc
    15481 ccaaagagaa tattttcccc aatgtaaaag gattaaaagg tccgttgggt tgcttgacaa
    15541 aagctcggta cggcattgcc tggggtgtta ttggtgcggc catggattgc tatgatacgg
    15601 cactgcgtta ttcaaaagaa agagaacaat ttggcaggcc tattggaggt tttcagttac
    15661 agcaaaagaa actggcggag atgatcacag agatcaccaa ggcacagttg cttaactggc
    15721 ggcttggggt attaatgagt gagggcaggg ccacaccaca acaggtaagc atggctaaac
    15781 gaaattcctg tgaaattgca acgaatatct gccgtgatgc aagacaaatg cttggaggca
    15841 tgggaatcac cggtgagtat cctgtgatgc gtcacatgat gaacctggaa agtgtgatca
    15901 cctatgaggg aactcatgat attcatttgc tgattacagg aatggatgtg accgggttga
    15961 atgcgtttaa ataaagtcag aagttggtaa gtccgaaagt cggaaagata gtctcaaata
    16021 attgagaatt tctttcggtc ttcctgactt tcggtctttc ggacaattct atacaatgaa
    16081 aatcttcctc attggtttca tgggctccgg taaaacacat tggggccgcc tgctgagtga
    16141 aaaacttggt atccgctttt ttgacctgga tgaacaggtg actgagcatg ccgggaaaac
    16201 cattccggag atatttgcgg cagaaggaga ggagcagttt cgcctactgg aaaaagatgt
    16261 tttgcatatc attactgaaa gccacgacaa ttttgtaatg gcctgcggcg gcggttctcc
    16321 ctgttatttc aataatattg aatacatgaa tactgccggc acgacagtct ggattaatac
    16381 accgcaggat acattgcaca aacgattagt agtagaaaaa gataagaggc cgctgattaa
    16441 agaccttaca gatgaacagt tgaggaattt tattcaaaag aaattctctg atcgcaggat
    16501 ttattacgaa caggccgata ttgcaattga tgaagaacct gttcaattag aaaaattaat
    16561 agaaaagata ttccatgtat aaaaattatt tggctattgc agcatttttt gccggactgg
    16621 ctgtggcctt aggggctttt ggggctcatg gtttgcaaaa gatcacttca gacgaaacaa
    16681 ttttacatag ttaccaaaca ggagtgcaat atcaaatgta tcatgcgctg ggtttgctgg
    16741 ccgttgctgt actatttgaa aaattttccc atcgctggct ccggtgggta gctaattgtt
    16801 ttattaccgg tatgatttta ttctcagggt cgctgtacct gctgactgta ctgaaaatac
    16861 agggcgctga aacgaaatgg gtgggtcccg taacgcctat aggaggttta ttctttatag
    16921 ccgggtggtt attattgctt gtggctgtaa caagaaaaaa tgactaaagc ggaagttcaa
    16981 gggtggttat tgttccattg ttattttcaa ttgtataggt acctccgata ctctccattc
    17041 gacgggcaat attttttagc ccgttgccga actggcggag tttttccata tcaataccct
    17101 tgccattatc ttttattaca atttggagtg tagcattcgc ttctatttct attgttacat
    17161 gggaggctgc agcatgcttc agcacattgt taagtgtttc cttgacgctt aagaaaatat
    17221 tgcgtcgttt atcgcctgtc agttcttttt ccggaatagt aacgggtacg gttactttac
    17281 attgcacagg agtattatca aagtattcga tggaatagga acgaatgtac gagatcaggt
    17341 tatcaagggt atcgttacca ctgttcatgc tccagataat agcattcatc ttattcagta
    17401 catcatcggc agagtgtgaa attttttcaa tttcaacggg ggtattctct ttcattttgt
    17461 tcctggcaat ttcactcatc agcctgatcg cagtcatgcc ggctcccagt tcatcatgca
    17521 tatcggcaga tatacgttgc ctttcttcct gctgcgcttt gtaaacagct aactcttttt
    17581 catactcttt taataaattt tgtgctttaa gggtttctct ttcttttgtc tgtacaataa
    17641 tatttttgcg gtttttataa ttgaggccgg ccaggaaaaa aaccagttcc aggaataagc
    17701 caacctggta ataaataagt gctgagccga gaatgccggg tagcgatttg aattgctgaa
    17761 ccatcagaat acctaactgt gagataaacg aaaaaataaa gagaaataaa ttaccccaga
    17821 ataaataccg cagaagtttg tccttccaat gacgggtact gtaaaaaagg aatatgatga
    17881 tcatacacag cagtaagatc ttcgtcaggt tctctaccag gaatgctgag ctgtaattat
    17941 cgctgaaata aaataaaaat gtgtaaatga acatggagca gcataacaaa ataatgccgg
    18001 cattatatag cgtggaaaga aaaggatgtt tgtttttggt gtccaggaat ttcttcatga
    18061 acaacatata aaaaatgata cccactccct gcaatataaa atctaaatag ccttcaagaa
    18121 aaaatccaat ttgactggag tgcaggtgat agatagtttg aaaaaataat aaaccaccca
    18181 ggaaaaacgc atacccggaa taatacaaga actcccggtt gcgcccctga aaaaaattgg
    18241 tgatagagaa gaggatcatc atcagcagca gtccgcaaaa gacataggtg atcaggttgg
    18301 tctcgttgta attactgtgg agtttcgcca aaaatagttt caggttgtct ttgccaataa
    18361 gcctgggctt aattccgtta ttatacgttt ttactgctct tagttctgct attattgttg
    18421 cagtatcatg ggcgggcaga cttataagcc gataaccggt tccttctgat atcggtgggt
    18481 ggacagcagg tataatttta aggccttcct gctccagtcg gtacatagtt attttgtcga
    18541 aataaaaacc gggacagaag aaaactgata taactgaatc agctgaatta caaatgttga
    18601 atttcagaat tatttttttc gataccatgc caattggaat aaggcctgtt tgctgcacgc
    18661 caggtttgaa ctgttcattg gctataattt gggtaatatc agctttgtga tctgtaaaat
//
LOCUS       DAORTI010000064        18649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2618_length_18649_cov_7.457513, whole genome shotgun sequence.
ACCESSION   DAORTI010000064 DAORTI010000000
VERSION     DAORTI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18649
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_18649_cov_7.457513"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1339
                     /locus_tag="PLO70_09465"
     CDS             14..1339
                     /locus_tag="PLO70_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQZ74736.1"
                     /translation="MSTIFKASMALLFPLIFPSGIVVNNAIIKDSNNSKHRIIKKPKM
                     GIQNASDTFIHSLLAKYPEFFSSALASRNKLGIQVIYTEINRSKKGKVKFTDHYFNLD
                     SGRYFYPASTVKLPVAILALQRLNELKIAGLDKNTTMITGAVGGQQTEVNNDPSAPDG
                     RPAIAHYIKKILLVSDNDAFNRLYEFLGQEYINNSLHKMGYASVQIIHRLDISLSEEE
                     NRHTNPIKFIDSSGKVIYEKAAEKSRLVYAQRDTKLGKGFYRGGQLVNEPFDFSKKNR
                     FALAELHGIVKSVMFPEAVPANRRFNLTKEDYAFLHQYMSMMPGESNSPVYDTADYWD
                     TYVRFLYYGSEKVKADPGIRIFNKAGDAYGFLIDAAYIVDLENNIEFLLSAVIHCNSD
                     EVYNDDKYEYESVGLPFLKNLGRVICQYERTRVRKNKPVLSSLIIDYSH"
     gene            1428..2618
                     /locus_tag="PLO70_09470"
     CDS             1428..2618
                     /locus_tag="PLO70_09470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQZ74737.1"
                     /translation="MQLSSFLQRFNEPETLKMAKLGRELRAKGIDVIDLSLGEPDFDT
                     PRHIKEAAVKAIQDNWSHYSPVPGFLDLREAVCTKLKRDNDLDYKPENIVVSTGAKQS
                     LANAMLALVDEGEEVIIPTPYWVTYSELVKIARGKVVEIKSTPQQGFKINAAQLEAAI
                     TPKSKLFLFSSPCNPSGAVYSRKELASLVEVFKKHPGILILSDEIYEYINYVGKHESI
                     AQFEEIKERVIIVNGLSKGFAMTGWRLGYIAATPEIAKACEKVQGQFTSGTNSITQKA
                     AVVALTTDLRPSMQMTEEFARRRKKVLEIVKAIPGITCPEPEGAFYIFPDVSAYYGKS
                     NGETTISNAADISMYLLNTAHVSSVMGDAFGEPKCVRFSFANSMEKIEEGWKRIKEAL
                     AKLK"
     gene            complement(2623..3369)
                     /locus_tag="PLO70_09475"
     CDS             complement(2623..3369)
                     /locus_tag="PLO70_09475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ74738.1"
                     /translation="MKRILAAIAFFITLVLPAIAQKPASSKVKLTENSVVKDTSGTTF
                     PYAIWNKLLYTGRYSIRPEYPQLENTAFIITRLSDEEFEKRMTSLPKPKESKYFKTGS
                     SFSHFKARDITGKKINTKELAGKTIVLNFWFINCPPCQKEIPDLHQLTETFKEDSSIV
                     FLAVALDQKHELKDFLKTHPFGYKIIDDGRYISNQYGLNSYPTNVVISPDGKVYFHSS
                     GLSSNTVYWLKKSIEELRQTALKNEPAGTD"
     gene            complement(3419..4006)
                     /gene="coaE"
                     /locus_tag="PLO70_09480"
     CDS             complement(3419..4006)
                     /gene="coaE"
                     /locus_tag="PLO70_09480"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793821.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQZ74739.1"
                     /translation="MIRVGLTGGIGSGKSTIAKIFETLGIPVYYADDAAKELMNSNED
                     LKQSVMQLFGKDSYKEGVLDRKYIASLVFNDTKKLELLNSIIHPATIKDAEAWLQQQT
                     APYAIKEAALLFESGASENLDYIIGVYAPRQLRIQRVIKRDGLPVEEILKRISRQINE
                     ELKMKLCDFIITNDEQEPVIPQVLALHQKLVSPKN"
     gene            complement(4108..4446)
                     /gene="yajC"
                     /locus_tag="PLO70_09485"
     CDS             complement(4108..4446)
                     /gene="yajC"
                     /locus_tag="PLO70_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQZ74740.1"
                     /translation="MFTFYLLQAKAESTGFGGFQFIFLGLMILVFWLFFIRPQAKRAK
                     NQKVFIENLGKGEKIVTIAGIHGTINKVNDDGTLNIEISPGSYIKIEKSAISMDWTAA
                     LNKPAAAEKK"
     gene            complement(4458..4910)
                     /locus_tag="PLO70_09490"
     CDS             complement(4458..4910)
                     /locus_tag="PLO70_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQZ74741.1"
                     /translation="MKNGALLLLVAFGFVACKNMDKKEAPVVTMSKEEIEKAKNDTTN
                     FTTIQWLDSTTQNLGKLTKDKEIEITWRFKNSGSKNLIIENVSASCGCTIPEKPEQPF
                     APGQEGTIKAKFNGSGSGTITKQVHVIANTNPAKDHTLTFVGEINDKQ"
     gene            complement(4980..5906)
                     /gene="nusB"
                     /locus_tag="PLO70_09495"
     CDS             complement(4980..5906)
                     /gene="nusB"
                     /locus_tag="PLO70_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQZ74742.1"
                     /translation="MISRRNIRVKVMQTLYTLSTLENEVKPGEPQKLLQQHFDQTRSL
                     LVYLIWFLTEVTRYAEKDAHQKASKHLPTAQDLNVNIKLAGNELLWKMMEDEALKEQF
                     TKEKPELIADRELIRKIYLTLTDTPEYKTYISNPSREKSEEKKILEFILNDLLLANEI
                     FVSHIEENFTNWDDDGEMVVQILTGYLQKPGHVAFKQMMSTDKEQFARNLLQTVWEKN
                     EHLQTLIIPKLKNWDPERIAQLDMIMMKMGVAEFLYFETIPPKVTINEYIDLAKDYST
                     QQSGQFVNGILDNIHKELVLQGKMQKVDYKKP"
     gene            complement(6145..9204)
                     /locus_tag="PLO70_09500"
     CDS             complement(6145..9204)
                     /locus_tag="PLO70_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HQZ74743.1"
                     /translation="MLKRHLILFLLLVVFTSAALQAQPPCGFDHLHSQLMERNPDYAR
                     QVELNEQSIQRYINAHPELLQRTGRIGALYTIPVVVHVMHTGGAVGTIYNPTDAQIIG
                     AINYLNQTFAGTYPGMTAPVEGGGIVNMEVQFALAQRTPSCGSTNGIDRVDASSLPNY
                     VANGVNVQTSSGCPELTMKNLARWNPADYYNIWIVNKLDGADGTSGQFIAGFAYFPGA
                     SSSLDGTVMLATQMTAGEKTLPHEIGHALNLYHPFQGSANNTQCPANANCTTDGDRVC
                     DTDPIFNNFNAGTGVYSFACRTGANVCAPPNNYTINTESNFMSYTNCYTLFTNGQKAR
                     AQAAMSLPSRASLVDPGNLALVPCGTTINFALATSSQTENISGTLSGCRRYTDYTYQM
                     TIGAAPTATATATLTYSGTAIKGLDYDVTTNGNFSSPSDVLTFNSGSTTAQSFTVRVY
                     DNASVESAETIILDFTVNNGGGDATKGTTTPTHTLTVSDNDQAPTGTSSGTYSIGTIA
                     FTVNGGPFDARQQSQRGQYLYKASELTAAGMTTGSITSLQLFINSKLSTRPFTNFTIK
                     MAHTNTANLVDGTVTVIGGMTTVYSSASVSTVAGWNSFSLGTAFNWNGSSNLAIEICF
                     DNTTADAGNFADQVGAYSDGGTASQGNMFFQNGVNCSGSFSSVTYYGTGIKPIIRLDV
                     SGTGTTIETIAGSTAANHIAVGSEDYFYSNNSRLLFRLSNVNASLGCVNASLEAGGTT
                     WVNSLGGQRSAKVFAVTPTTNFSTTGYTISLYFDNTELDGKNPALLRIAKTTAASAAA
                     ANATNTVLVTPTVTTLGSGTTVFTAPFTGFSRFFLVDAAVVLPVSLTEFTATANSAQN
                     TVVKWVTSFEQHNRDFTIEVSNDGINFVPLATVASKGNSTTVQQYEYLHIKPAKGTTW
                     YRLKQTDLDDNFRYSRIVAVDISKGQTKAFLYPVPAKDILTINFGTPVNKCAIEIYTP
                     NMQVIQRENISSLSLKKEINVSALPAGFYFVRMTSAAGTELLRFVKE"
     gene            9474..9755
                     /locus_tag="PLO70_09505"
     CDS             9474..9755
                     /locus_tag="PLO70_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQZ74744.1"
                     /translation="MAKKVNVTPLHDRVIVKAAAAEEKTAGGIIIPDTAKEKPQRGTV
                     IAAGPGKKDEPVTVKTGDTVLYGKYAGTEIQIDGDDYLIMRESDILAIV"
     gene            9858..11492
                     /gene="groL"
                     /locus_tag="PLO70_09510"
     CDS             9858..11492
                     /gene="groL"
                     /locus_tag="PLO70_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQZ74745.1"
                     /translation="MAKQIFFDIEARNKMKKGVDTLANAVKVTLGPKGRNVVIEKKFG
                     APQVTKDGVTVAKEIELEDPIENMGAQMVKEVASKTADLAGDGTTTATVLAQSIISEG
                     LKMVAAGANPMDLKRGIDKAVSLVIESLKSQSQTVGSDSKKIQQVATISANNDETIGK
                     LIAEAFIKVGKEGVITVEEAKGTDTTVDVVEGMQFDRGYISPYFVTNSEKMEAELQNP
                     YILIYDKKISNMKDILHILEKVAQGGRPLLIIAEDLEGEALATLVVNKLRGTLKVAAV
                     KAPGFGDRRKEMLQDIAILTKGIVISEEQGYKLENADLTYLGQATSVTIDKDNTTVVG
                     GKGKKEDIVARVNQIKAQIESTTSDYDKEKLQERLAKLSGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVAYIRAIDSLEAKVRGQIADEQTGMAIVRRALEE
                     PIRILTANAGIDGSIVVQKIREGKGDFGFNARTEVYENLFKAGVIDPTKVSRVALENA
                     ASIAGMLLTTECVVADKPKKEEPHAHGAPDMGGMGY"
     gene            11606..12625
                     /locus_tag="PLO70_09515"
     CDS             11606..12625
                     /locus_tag="PLO70_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74746.1"
                     /translation="MKTQRKFRPFFVVIILCLTFFSCKKTGTETTKEQTVKEGYSQNG
                     SHQQNVLRYNRPTDAVKIFSKTIDVPPGKGVGKPSNNTSTAGRGPCNYTFSGTLEYAI
                     YSLPCGTLNMPSGYPTNEYRIDFTFYMNTIGSSLPNLQNDFLILNGVTLSNPGDYSYS
                     SGVPPTSQNPNGDGFTFTGSIWFSNLGINLPIGCGQSTTSMIFQGHCWSPLCSPGDDY
                     FITINNISLKPSDACAANSEPAIVYPNVSPNQGEFYVFMPWDGACFETCFLPDDWTDL
                     EIEYYKDPGGTHYNVLTGYVPFTYGPYIVSVLDGPGNYSIRSRYKCPGGGSGAWSTWS
                     SVPVY"
     gene            12832..>16764
                     /locus_tag="PLO70_09520"
     CDS             12832..>16764
                     /locus_tag="PLO70_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQZ74747.1"
                     /translation="MRKIYKLSLTVFAALTISFSLFAQNKFFTDAGENRTVQTTGQRV
                     IIPQKFRTSFMDVLAMKDFLWSLPSEQAARANRSQMPVIELPTPDGGMAKFLVWESSI
                     QEPALEAKFPDIKTFTGQGIDDPFATIRLDFGPNGFHGQVLTVNGSYYIDPYARGVLN
                     HYISYYRTDFRKSGKFLCEVPDNPSFSRNNAIEAACLGTDLRTYRLAVACTGEYAQAP
                     GIAAGANPAILHAAIVTTVNRVVGVYEKEVSIRMILVANNNLVEFLNAATDPFTGNNN
                     ANVLINESQTVIDANIGAANYDIGHTFSTGGGGLAQLNSPCGPSKARGITGSPFPTGD
                     AYDIDYVAHEMGHQFGGNHSMAGCGSSPASTKYEVGSGTTIQAYAGICGAEDIQPNSD
                     PYFHAISFDEISNFIVSGGGSTCGTSAPTGNNLPVIAPLANNGVSIPPGTPFTLSGSA
                     TDADGDPLTYNWEQWDLQGTQTWNAGATAAPGNTVPLFKSRVPKTNGSRTFPDIAVIL
                     ANFPANPAATMGGLKGETLSPVARPMKFRLTVRDNRAAGGGVTSSGSSGCQSSTTYQI
                     NVVGTTPFTVSSPNGGESFQGGSTQTITWNNAGTNVAPYNVTNVRISLSTDGGLTYPT
                     EIVASTPNDGSETLTIPVVITSTARIKIEAVGNIFFDISNANFTITPPPTGFSFGPTS
                     PATATCPAPATMNVVLPTIPNGGFTNPITLAATAGVPAGTTITFAANPVLPGNNGLVT
                     LNNANTLSPGTYVVTISGTATGAPNQNANVTFTVNAGAGPAITTQPSNQTACVGTNAT
                     FSVVATGTYQWQLNTGSGWNDISGATSASYTVNGVTLTQNGYTYRCVVTGQCGSTNSN
                     VVTLTVNALPAITAQPQSLNLCSGSAASFSVTATGAGLSYQWELSTNGGGTYTPITGA
                     TSATYNIAAVAVTDNNNMYRCVVTGTCAPAATSSAATLLVSSSITVTTNPVSQTVCEG
                     TNLSFTVAAGGSGLSYQWEVSTNGGTSWSNIANGGVYTGATTPTLNITGVPPTFNTYR
                     YRATVSNAACTPGVSTVATLTVNTFPVITAQPQNATICEGASQSFSVTATTGVGTLSY
                     QWQLSVNGGTSWTNLAGATSSTFAQTAIPAGQNSYRFRVIVTAGCGSVTSNAAVLTVN
                     TYPVISFTAPAVTCVSDASFTLSAGPVGGVFSGPGVGGTTFNPSAAGVGPKTVTYTAT
                     NNGCQSSIGRVILVNECAERHLTLDQYPAIQVFPSPNNGRFSIRLNTDLYTKLNIKIF
                     NSAGQMVRSQIANGLTFGSVIPLDITNQASGTYHLFLSNDENGSISTKG"
     assembly_gap    16765..16864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17051..>18649
                     /locus_tag="PLO70_09525"
     CDS             17051..>18649
                     /locus_tag="PLO70_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ74748.1"
                     /translation="MISVKDLKLAYGKRVLFEEVNLNFTKGNCYGVIGANGAGKSTFL
                     KILSGEIEPNKGTVEITPGERMAVLKQNQFQFDEETVLNTVMMGHTKLWAIAKERETI
                     YALADFTEEDGMRAGELEHEFGEMGGYTADSDAATLLSELGVKEEYHQSLMKDIPSNL
                     KVRVLLAQAIFGNPDILLLDEPTNGLDIETIGWLENFLADYENIVLVVSHDRHFLDAV
                     CTHVADVDRQKIKIFTGNYTFWYESSQLMARQINDKNKKTEEKRQALIDFIARFSANA
                     SKSKQATSRKKALEKLSIEEIEPSYRKYPGIIFQQLREVGNQILNVDKLKKSVDGRVL
                     FSDVTFTVNKGDKIALLSRDPLAVTSFFNIITGDTKTDSGSYEWGTTVTHAYLPQENN
                     HFFNGKDNLMDWLRQYVPDHVKDVDEPFLRGFLGKMLFSGDDVLKTTNVLSGGEKVRC
                     MLSKMMLQSPNVILLDQPTNHLDLESIQSFNEQCTDYKGIVLLTSHDHTFMQTVANRV
                     IELTPKGIIDRLMTFDEYLVDERVK"
BASE COUNT         5297 a         3785 c         4123 g         5344 t
ORIGIN      
        1 tctttaaaag gcaatgtcaa caatctttaa agccagtatg gctcttttat ttcccctgat
       61 tttcccttca ggaattgtag tcaataatgc cattataaaa gactcaaata actccaaaca
      121 taggataata aagaaaccaa aaatggggat tcaaaatgca tctgatacat tcattcacag
      181 tcttttagct aaatatcctg aattcttcag ctctgccctg gccagcagga ataaactggg
      241 catccaggtg atttatacgg agataaacag gtcaaaaaag gggaaagtta agtttacaga
      301 tcattatttt aatcttgatt cgggccgcta tttttatcct gcatccactg ttaaacttcc
      361 cgtggcgata ctggccctgc agcgattgaa cgaactaaaa atagccgggc tggataaaaa
      421 cacaacgatg attaccggcg ctgtgggcgg tcaacaaaca gaagtaaaca atgacccttc
      481 tgcacccgat ggaaggcctg ccattgccca ttatataaaa aaaatactgc tggttagtga
      541 caacgatgct tttaaccggt tgtatgaatt cctgggccag gaatatatta ataattcatt
      601 gcacaagatg ggttatgcca gcgttcagat cattcataga ctggatataa gtttatccga
      661 agaggaaaac aggcatacca atcccataaa attcatcgac agcagcggga aagtaattta
      721 tgagaaagct gctgaaaaaa gcaggttggt ctatgcgcaa agagatacaa agctgggaaa
      781 aggattttac aggggcggcc agctggttaa tgagcctttt gatttttcaa aaaagaaccg
      841 gttcgcattg gctgaattgc acgggatagt taagagtgtt atgttcccgg aggcagtgcc
      901 tgcgaatagg cgtttcaatc ttaccaaaga agattatgca tttctccatc agtacatgtc
      961 gatgatgcct ggggaatcca actcgccggt ttatgatacg gctgactatt gggatactta
     1021 tgtcaggttt ttgtattacg gttctgaaaa agtgaaagca gatccaggca tccgtatttt
     1081 taataaagca ggagatgcat atggcttttt aattgacgct gcttacatcg ttgacctgga
     1141 aaataatatt gagtttttac taagtgctgt tattcattgt aacagtgatg aggtttataa
     1201 tgacgataaa tatgagtatg aatctgtcgg gttacctttc ctgaaaaacc tgggtcgggt
     1261 tatctgtcaa tatgaacgaa caagggttag gaaaaataaa ccagtgcttt cttctcttat
     1321 tattgactac agtcattgaa tcattattat caatttccat atcttcacgc acctgttttt
     1381 agcgacggcc tgccatcatt gtttaataat aaaatctgat tatttccatg caattatctt
     1441 cttttttgca acgcttcaat gaaccagaaa cattgaaaat ggctaagctt ggccgtgaac
     1501 taagagccaa agggattgat gttatagatc tgagtttagg ggaacctgat tttgacacac
     1561 cccggcacat caaagaagct gctgttaaag cgatacagga taactggagc cactacagcc
     1621 cggtgcccgg gtttcttgat ttacgggaag ctgtttgtac aaaactgaaa agagataatg
     1681 atcttgacta taagcctgaa aatatagttg tatctacagg tgccaagcaa agtctggcga
     1741 atgcaatgct tgcattggta gatgaaggag aggaagtgat aattcccacc ccttactggg
     1801 ttacatatag tgagctggta aaaattgcaa ggggtaaagt ggtggagatc aaaagtacac
     1861 cacagcaggg attcaaaata aatgcagccc agctggaagc tgctattact ccaaaatcaa
     1921 aactgttcct gttctcctct ccctgcaacc caagtggtgc tgtttacagc aggaaagagc
     1981 tggcttcatt ggttgaagtt tttaaaaaac atccgggtat cctcatcctg tcagatgaga
     2041 tttatgaata tatcaactat gttggtaaac atgagagcat tgcacagttt gaagaaatta
     2101 aggaaagggt catcattgta aacgggctga gtaagggatt tgccatgaca ggctggaggc
     2161 tgggttatat agctgctact ccggaaattg caaaagcctg tgaaaaagta cagggacagt
     2221 ttaccagcgg tactaattca atcactcaaa aagctgctgt tgttgcactc acaactgatt
     2281 tgcgtccatc aatgcagatg acggaggagt ttgccaggag aagaaaaaaa gtactggaga
     2341 tcgttaaagc aataccaggt attacctgtc ctgaacccga aggggctttc tatattttcc
     2401 cggatgtaag tgcttactat ggtaaatcaa acggcgaaac aacgatcagc aatgcagctg
     2461 atatttccat gtatcttttg aatacggccc atgtttcctc tgtaatgggg gatgcatttg
     2521 gagagccaaa atgtgttcgc ttttcatttg cgaacagcat ggaaaaaatt gaagaagggt
     2581 ggaagcggat caaagaggcg ttggctaaat taaaataaca atttagtcag tacccgccgg
     2641 ttcattctta agggcagttt gcctgagttc ttcaattgat ttcttcagcc agtaaacagt
     2701 gttactgcta aggccggagc tgtggaaata aacttttcca tccggcgaaa tcaccacatt
     2761 ggtcgggtag gagtttaacc cgtattggtt ggatatataa cggccatcat caatgatttt
     2821 atagccaaac ggatgtgttt tcagaaaatc tttcaattca tgtttttgat ccagcgccac
     2881 ggcaaggaac acaatcgagc tgtcttcctt aaatgtttct gttaattgat gcaggtcagg
     2941 tatttccttc tgacaaggcg ggcaattgat aaaccagaaa tttaaaacga tcgttttccc
     3001 ggcaagctct tttgtattta tttttttgcc ggtaatatcc cttgctttaa aatgggaaaa
     3061 actacttcct gttttgaagt atttgctttc ctttggtttt ggcagggagg tcatcctttt
     3121 ttcaaattct tcatcggaaa gcctggtaat aataaatgca gtattttcta attgtggata
     3181 ttcaggtctg atactgtagc ggcctgtata caataattta ttccagatgg cgtaggggaa
     3241 tgtggtgccg cttgtatctt ttactaccga attctccgtg agcttcactt ttgaggatgc
     3301 cggtttctgg gcgattgcgg gtaatacaag ggtgatgaaa aatgcaatgg cagcaagtat
     3361 tcttttcatg ataaggtttt gatgttttaa gatatactta ttgggaattc ttaaagtatc
     3421 aattttttgg ggatacgagt ttctggtgca gggccagcac ctgtgggatc accggttcct
     3481 gttcatcatt ggtgataata aaatcgcaaa gtttcatttt tagctcttca ttaatctggc
     3541 ggcttatgcg tttcaggatc tcttccaccg gcagaccgtc tctttttatt acccgttgta
     3601 tacgtagctg ccggggtgca taaaccccga ttatatagtc tagattttcg gatgcgccac
     3661 tttcaaaaag caaggcagcc tccttaatgg cataaggtgc ggtttgttgc tgcaaccagg
     3721 cttccgcatc ttttatggta gccggatgga taatcgaatt gagcagctcc agtttttttg
     3781 tatcattaaa aacaagtgac gcaatatatt tcctgtcaag cactccctcc ttataactgt
     3841 cttttccaaa aagctgcatg accgattgtt tcaggtcttc attgctgttc attaattctt
     3901 tagcggcatc atctgcataa tacacaggta tgcccagcgt ttcaaatatc tttgctatgg
     3961 ttgatttccc gctaccaata ccacctgtta gaccgacacg aatcattaat ttgtttaatt
     4021 attatattgg aatgacaatt cagttcgtaa ggtaaaaaca aaaccggatg attacacccg
     4081 gttttgtttt tttcatctga tcagcgatta ttttttttca gctgctgctg gtttattcag
     4141 ggctgctgtc caatccatac tgatagctga tttttcaatt tttatatagc tgcccgggct
     4201 tatttcaatg ttcagtgttc cgtcatcatt cactttattg atagtaccgt gaataccggc
     4261 aatagtgacg attttttcac ctttgcccag gttctcaatg aacactttct ggttcttagc
     4321 ccttttagcc tgcgggcgga taaagaacag ccagaacacc aggatcatta aacctaaaaa
     4381 gataaactgg aaaccaccaa aaccggtcga ctcagccttt gcctgtaata aataaaatgt
     4441 aaacatgctt tattgtttta ttgtttgtcg ttgatttctc caacgaatgt taatgtatga
     4501 tcttttgcag gattggtatt ggcgatcaca tgtacctgct tggtgatcgt accacttccg
     4561 ctgccattaa attttgcttt gattgtacct tcctgcccgg gggcaaaagg ttgttccggc
     4621 ttttccggga ttgtacatcc gcaggaagca gatacgtttt ctatgataag gtttttagaa
     4681 cccgaatttt taaagcgcca ggttatttca atttctttat ccttagtaag ctttcccagg
     4741 ttctgggttg tcgaatcgag ccattgaatg gtcgtaaagt tggtggtatc attcttcgct
     4801 ttttcaattt cttctttact cattgtgaca acaggcgcct cctttttgtc catatttttg
     4861 caggcaacaa aaccaaatgc taccagcagt aataatgctc catttttcat tttcaatgtt
     4921 tttagttcca gataagttat tgggattgca aatgtagaaa aaactgttga gctggccgtt
     4981 tatggttttt tgtaatctac tttctgcatt tttccctgca gcaccagctc tttgtgaata
     5041 ttatccaata ttccattgac aaactggccg ctttgctggg tgctgtaatc cttggccagg
     5101 tcaatatact cgttgatggt tactttgggc gggatcgttt caaaatataa aaattcagca
     5161 acacccattt tcatcatgat catatccagc tgggcaatac gttccgggtc ccagtttttc
     5221 agttttggaa tgatcagtgt ttgcaggtgc tcattttttt cccaaacggt ttgcagcagg
     5281 ttccgggcaa attgctcctt gtccgtactc atcatctgct taaacgctac atgaccgggt
     5341 ttttgaaggt agccggtcag gatctgtacc accatttcac cgtcatcatc ccagttagta
     5401 aagttttctt caatatgtga aacaaaaatc tcattggcca gcagcaggtc attgagaata
     5461 aattcaagga tcttcttttc ctcgcttttt tctcttgatg ggttactgat atatgttttg
     5521 tattccggcg tatcagtgag ggtaaggtag atctttcgaa tcagctccct gtcggcaatt
     5581 aattcgggct tttctttggt aaattgttct tttaacgcct cgtcttccat catcttccac
     5641 aataattcat tgccggcaag tttgatattt acattcaggt cctgtgccgt aggtaaatgt
     5701 ttggaagctt tctggtgcgc atctttttca gcataccggg ttacttctgt gagaaaccaa
     5761 ataagataaa caagaaggga tcgggtttgg tcgaaatgct gttgtaatag tttctgtggt
     5821 tcaccgggct tcacttcatt ttctaatgtg gaaagagtgt acagtgtttg catcactttt
     5881 acccggatat ttcttctgct gatcattact taagaactat ttgaagcggc aaagataggg
     5941 aagcagcttc gtattgctgc attatgctta ctataattaa gaacggaaaa aagaggctgt
     6001 caaaaaagca aacaaatttg tcacattgag tttatcgaaa tgcgtacaaa tttgtttacc
     6061 aatcattgtc atatttcggc tatgctcaat atgacaatga ttgggctttt tagacagcct
     6121 cctgtaaaca attatttcaa tacgttattc ttttacaaac cggagcagct cagtgcctgc
     6181 agcgctggtc atcctcacaa aataaaaacc ggcaggaagt gcactaacat ttatttcttt
     6241 ttttaatgac aggctgctga tattttcccg ttgaatcacc tgcatgttcg gcgtatatat
     6301 ttcaatagca catttattaa ccggtgttcc aaaattaatt gtcaggatat cttttgcggg
     6361 cacaggataa aggaatgctt ttgtttgtcc tttgctgata tccaccgcaa cgattctgct
     6421 gtacctgaag ttgtcatcca ggtcggtctg tttcaaacga taccaggttg ttcctttggc
     6481 cggttttata tgcaggtatt catattgctg tacagttgtt gaattgcctt ttgaagccac
     6541 tgttgccagc ggaacaaaat ttattccatc attactgact tcaatagtga agtccctgtt
     6601 atgctgttcg aaactggtga cccattttac tacagtattt tgagcactgt ttgcagttgc
     6661 cgtaaattcg gtgagtgaaa ccggaagaac aacagctgca tccaccagga agaaacggga
     6721 gaacccggta aaaggggcag taaatacagt ggtgcctgat cccagcgtgg taacagttgg
     6781 cgttaccagc actgtattag tcgcattggc tgcagcggca gaggcggctg tagtttttgc
     6841 aatacgcaat aaagcagggt tcttaccatc cagctctgta ttgtcaaaat ataacgagat
     6901 agtataacct gttgtggaga aattggtggt tggggttacc gcaaacactt ttgctgaacg
     6961 ttgcccaccc aatgaattga cccaggtagt accgccggct tccagggaag cattgacaca
     7021 gcccagtgaa gcattgacat tacttagcct gaacaagagc ctgctgttat tggaataaaa
     7081 ataatcctca ctgcccactg caatatgatt ggcagcggtg gagccggcaa ttgtttcaat
     7141 ggtcgtaccc gtgccggaaa catccagcct gatgatgggt tttatacccg tgccataata
     7201 tgttacggaa ctgaaagagc cggaacaatt gactccgttc tgaaaaaaca tgtttccctg
     7261 cgaagcagta ccaccatcag aataagcccc cacctggtct gcaaaatttc ctgcatctgc
     7321 tgtagtatta tcaaaacaaa tttcaatggc aagattgctg ctgccattcc agttaaaagc
     7381 tgttcctaat gaaaaactgt tccagcctgc tactgtcgaa acagatgcgc ttgagtaaac
     7441 tgttgtcatt ccgccgatca ccgtcacagt tccatctacc aggttggcag tgttggtatg
     7501 tgccattttt attgtgaaat tggtaaaagg ccttgtgctt aattttgaat taataaaaag
     7561 ttgcagggag gttatacttc ctgttgtcat tcccgccgct gtcaactcac tggctttata
     7621 caggtattgt ccccgctggc tttgttgcct tgcatcaaaa ggtcccccgt taacggtgaa
     7681 agcaatggta ccaatagaat atgttccgct tgatgttccg gtaggagcct ggtcattgtc
     7741 ggaaactgtc agggtatggg taggagtggt agttccttta gtggcatcac cacctccgtt
     7801 atttacagta aaatccagta tgatcgtttc agcagattct acacttgcat tatcatacac
     7861 tcttacggta aaactttgag cagttgttga gccgctattg aaagtaagaa catcactcgg
     7921 gctgctgaaa ttaccattgg tggtcacatc atagtcaagc ccttttatcg ctgtaccgct
     7981 ataagtaagt gttgcagttg ctgtagctgt tggggcggca ccaatcgtca tctggtaagt
     8041 atagtcagtg taacggcggc agccacttaa tgttccggaa atattttctg tttgggaaga
     8101 ggtggccagc gcaaaattta tcgttgtgcc acagggtacc agcgcaaggt ttccggggtc
     8161 taccaggctt gcccttgatg gcaaggacat agcggcctgt gcccttgctt tttgtccatt
     8221 ggtaaataat gtatagcaat ttgtataact cataaaattg ctttctgtat tgatcgtgta
     8281 attgttggga ggggcgcaaa cattggcacc ggtgcggcag gcgaagctgt atacacctgt
     8341 tccggcattg aaattattaa agatcgggtc tgtatcacaa accctgtcgc cgtcagttgt
     8401 acaatttgca ttagccggac attgcgtatt gttagctgaa ccctggaagg gatgatagag
     8461 attaagggca tgaccaattt cgtgcggtaa agttttttcc cctgcggtca tttgcgtagc
     8521 cagcatcaca gttccgtcca gcgaggagga agctccgggg aaataagcaa agcctgcaat
     8581 aaactgaccg gatgtgccat ctgcaccatc aagcttattt actatccata tattataata
     8641 atcagcaggg ttccaccggg ccaaattctt catcgtcagt tcggggcagc cggaagaggt
     8701 ttgaacattg acgccattcg caacatagtt gggaagggaa gaagcatcaa ccctgtcaat
     8761 accattcgtt gagccacagg atggggttcg ttgggccagg gcaaactgca cttccatatt
     8821 tacaataccg ccgccttcaa cgggagcagt catcccggga taggtacctg caaaagtctg
     8881 gttgagataa ttgattgcac caatgatctg cgcatccgtt gggttataaa tagtgcccac
     8941 tgccccgccg gtgtgcataa catgtacaac aacgggtatg gtatataatg ccccgatgcg
     9001 gccggttcgt tgcagcagtt ccgggtgtgc attgatataa cgctgaattg actgttcatt
     9061 cagttcaacc tggcgggcat aatccgggtt gcgttccatc aattgagaat gtaaatggtc
     9121 aaaaccgcag ggaggttgtg cctgtaaagc tgcagatgta aacacaacaa gcagtaaaaa
     9181 aaggatcagg tgtctcttca gcataggata ttagtattaa aaaacaaata taacggtcac
     9241 aatcaacaga tgcaaccttc tttattatac gccggtaaaa gtgaaaaatt gtcttgttat
     9301 cgccataaag tgtaagtttt tcagtagtaa ttctgtaacg gaatgaaaaa atgtcctttt
     9361 tacccctccg gcatagtatt tactgtacct tcgccgccca tcatcaggaa aatttgatgg
     9421 gagacctgtt gaaaggtctt ttatagcatt caaaaactca aaatcaatac attatggcta
     9481 agaaagtcaa tgttacccca cttcatgaca gggtaattgt aaaagctgcc gcggcagaag
     9541 aaaaaacagc tggtggtatt atcatccccg ataccgctaa ggaaaaacca cagcgtggta
     9601 cggtgatagc agcaggtccc ggtaaaaaag acgaacctgt aacagtaaaa accggagaca
     9661 cggttttata tggcaaatac gcaggaacag agatccagat cgatggtgat gattacctga
     9721 tcatgagaga gtctgacatt ctggcgatag tttaagcagc ctcccgttag caatcgggat
     9781 tggcaataag acaatctgcc aattaaaaaa atcattgtca aattatcgaa ttaactaatt
     9841 atcaaatttt aataattatg gcaaagcaaa tcttcttcga cattgaagca agaaataaaa
     9901 tgaaaaaagg cgtggacaca ctggccaacg ctgtgaaagt gacattaggc cctaaaggcc
     9961 gtaatgtggt tatcgagaaa aaattcggtg caccacaggt aactaaagat ggtgttaccg
    10021 ttgctaaaga aattgaactg gaagatccta ttgagaacat gggagcccag atggtaaaag
    10081 aagtagcttc taaaactgct gaccttgcag gtgatggaac tacaactgcc actgttttag
    10141 cccagtctat catcagcgaa ggattgaaga tggtagctgc cggtgccaac ccgatggatc
    10201 tgaaaagagg catcgacaag gcagttagcc tggttatcga aagcctgaaa agtcaaagcc
    10261 agactgttgg cagcgacagc aaaaaaattc agcaggtggc tactatttct gctaataatg
    10321 atgaaacaat cggtaagctg atcgcagaag ctttcatcaa agttggtaaa gaaggtgtga
    10381 tcaccgtaga agaggcaaaa ggcactgaca ctacagttga tgtagtggaa ggtatgcagt
    10441 tcgatcgtgg ttatatctct ccctacttcg taacgaacag cgaaaaaatg gaagctgaac
    10501 tgcaaaaccc ttatatcctg atctacgata agaagatcag caatatgaag gatatcctcc
    10561 atatcctgga gaaagttgcc cagggcggac gtccgttact gatcattgct gaagacctgg
    10621 aaggcgaagc attggcaaca ctcgttgtga acaaactgcg tggtacattg aaagtggctg
    10681 ctgtaaaagc tcctggcttc ggtgaccgca gaaaagaaat gttgcaggac attgctatcc
    10741 tgacaaaagg tattgtgatc agcgaagagc agggttacaa actggaaaat gctgacctca
    10801 cttatcttgg acaggctacc tctgtaacaa ttgataaaga caacactacc gttgtgggtg
    10861 gcaagggcaa gaaagaagat atcgttgccc gtgtaaacca gatcaaagca cagatcgaat
    10921 caactacttc cgattacgac aaagagaaat tacaggaacg cctggctaaa ttaagcggtg
    10981 gggtagctgt attatatgta ggtgctgcta ctgaagttga aatgaaagaa aagaaagacc
    11041 gtgttgacga cgcactgcat gctacaaggg ccgcagtaga agaaggtatc gttcccggtg
    11101 gtggtgtagc ttatatccgt gctatcgaca gcctggaagc taaagttcgt ggccagatcg
    11161 ctgatgagca aaccggtatg gccatcgttc gccgtgcact ggaagagcca atccgcatcc
    11221 tgaccgctaa tgctggtatt gatggttcta tcgtggttca aaagatcagg gaaggtaaag
    11281 gagattttgg ttttaatgcc aggactgaag tgtatgagaa ccttttcaag gctggtgtga
    11341 tcgatcctac aaaagttagc cgtgttgcct tagagaatgc tgcttccatc gcaggaatgt
    11401 tgctgactac tgaatgtgtg gttgccgaca aaccaaagaa agaagagcca catgcacatg
    11461 gtgcacctga tatgggtggt atgggatatt aatagtatta ccatagtatt aaagtttagg
    11521 aaaagacctg ttttttaaca ggtcttttcc tattgacggc attatcaaaa agatataatt
    11581 ttatttctca tcaaaacttt aaactatgaa aacacaaaga aaattccgtc ctttttttgt
    11641 tgtcattatt ttgtgtttaa cttttttttc atgcaagaaa actggtacag aaacaacaaa
    11701 agaacagact gttaaagaag gttattcaca gaacggaagt catcaacaaa atgtattgag
    11761 gtataataga cctacagatg ctgtaaagat tttttctaaa acaattgatg tacctccggg
    11821 taaaggagtt ggcaaaccct caaataatac atctacggct ggtagagggc catgcaatta
    11881 tacattctct ggtactttag agtatgctat ttatagctta ccatgtggta cattgaatat
    11941 gccatcagga tatccaacta acgagtatcg tattgatttt acattttaca tgaatacgat
    12001 aggttcttcg ctgccaaatc tccagaacga ttttcttatc cttaatggtg ttacattgtc
    12061 caatcccgga gactattctt attcttctgg tgtgccccca acttcacaaa accctaatgg
    12121 cgacggtttt acatttacag gctcaatttg gtttagtaat ctgggtataa acttgccgat
    12181 tggctgtggc cagtcaacta cttcaatgat atttcagggg cattgctggt ctccactttg
    12241 tagtccagga gatgattact ttattactat taataatata tccctcaaac cctctgatgc
    12301 ctgtgctgct aattccgaac cagcaatagt ttatccaaat gtttctccta atcagggtga
    12361 attctatgtg ttcatgccat gggatggagc ttgctttgaa acatgttttt tacccgatga
    12421 ttggaccgat ttggaaattg aatattataa agaccctggt ggtacacatt ataatgtact
    12481 aactggttat gttcctttca cctatggacc gtatatagtt tcggttttag atggcccggg
    12541 aaattatagt attcgaagca ggtacaaatg cccaggtgga ggaagtggtg cgtggtccac
    12601 ctggtcgtct gttcctgttt attagcagac ccgtcgatct tcgatactgc ttagtataaa
    12661 ttgattcaat gtgagaaatc ctgtttttta tttaaaaaca ggattttttg cattagtgaa
    12721 aaattgggtt cgcagaatat gaattattct tatttttaac actctatttt gtcttgattg
    12781 cttaaaaaca ttcctgtttt tattagtcat tcacttaact aaaaaaataa tatgagaaaa
    12841 atttacaaac tcagtcttac ggtttttgct gctttgacta tctcattttc tttgtttgca
    12901 cagaataagt tcttcactga tgctggtgaa aacagaacgg tgcaaacaac tggtcaaaga
    12961 gtaattattc cacaaaaatt ccggacatct tttatggatg ttctggcaat gaaagatttt
    13021 ctttggtcgc ttccttcgga gcaggctgca agagccaacc ggagtcaaat gcctgtaata
    13081 gaattaccaa ctcctgatgg agggatggca aaatttctgg tttgggaaag tagtatccaa
    13141 gagccagctc tggaagcaaa atttccggat attaaaacat ttacgggaca gggaatagac
    13201 gatcctttcg caacgattcg gcttgatttt ggcccgaacg gatttcatgg gcaggtatta
    13261 actgtaaacg gatcatatta catagatccg tacgcaagag gtgttttgaa tcactatatc
    13321 agttattatc gcacagattt cagaaaatcc ggaaaatttc tttgtgaagt gcctgataat
    13381 cccagctttt cccgtaataa cgcaattgag gctgcatgtt taggtactga tctcagaact
    13441 tacaggctgg cagtagcctg tacgggcgaa tatgcacaag caccgggtat tgcggctgga
    13501 gcaaatcctg caattttgca tgctgcaata gttaccactg tcaatagagt agtaggggtt
    13561 tatgaaaaag aagtttctat aaggatgata ctggtagcaa ataacaatct ggtagaattt
    13621 cttaatgctg caacagatcc ttttaccgga aacaataatg caaacgtttt gattaacgag
    13681 agtcagacag taattgacgc taatatcgga gctgctaact atgatattgg ccatacattc
    13741 agtacgggcg gcggcggctt agcacaattg aattcgccct gtggtcccag taaagcaaga
    13801 ggtataacag gaagcccttt tcctacaggg gatgcttatg acatagatta tgtggcgcat
    13861 gaaatgggac accagtttgg aggaaatcac tctatggcgg gatgcggatc atctcctgca
    13921 tctactaagt atgaagtagg aagcggtacc accattcagg cttatgcggg tatatgtgga
    13981 gcagaggaca tacagcctaa tagcgatcct tactttcatg caatcagctt tgatgaaata
    14041 agcaatttca ttgtttctgg tggtggcagt acctgcggta ccagcgcacc tactggtaac
    14101 aatttgcctg ttattgctcc tttagcgaat aatggagtta gtataccacc gggcactcct
    14161 ttcacattat ctggatctgc taccgacgca gatggagatc cgcttactta caattgggag
    14221 caatgggatt tacaaggcac tcagacatgg aatgccggtg ccactgcggc tccaggaaat
    14281 accgttcctc tgttcaaatc aagagttcca aaaactaacg gtagcaggac ctttcctgat
    14341 atagctgtta tattggcaaa tttccctgct aaccccgctg caacaatggg tggtttaaaa
    14401 ggagaaacat tatcaccagt tgcccgccca atgaaattca ggctaactgt aagagataat
    14461 cgtgctgctg gcggcggcgt tacttccagt ggttcatccg gatgccagag ttccactact
    14521 taccagataa atgtagttgg tactactcct ttcactgtta gctcaccaaa tggtggtgag
    14581 tcttttcagg gaggctctac acaaacaatt acatggaata atgcgggtac taatgtggct
    14641 ccttacaacg taacgaatgt gaggatctca ttatctacag acggtgggtt gacttatcca
    14701 actgagattg tagccagcac acctaacgat ggttctgaaa cattgaccat acccgttgta
    14761 ataacttcta ctgcacggat caagattgaa gctgttggaa atatcttctt tgatatttct
    14821 aatgccaatt tcactatcac tcctccgccg accggcttta gcttcggacc tacttcacct
    14881 gctacggcaa cttgtccggc tcctgccaca atgaatgtgg tattaccaac tattcctaac
    14941 ggaggattta ccaatccaat tacattagca gcaactgcag gagttcctgc cggtactact
    15001 attacatttg cagccaatcc tgttttaccg ggtaacaatg gcttagtaac attaaataat
    15061 gcaaatacat tatctcccgg tacgtatgta gtaactattt caggaactgc aaccggtgca
    15121 cctaaccaga atgctaacgt tacctttact gtaaatgcag gagcaggacc agccattact
    15181 acacagccat ccaaccaaac tgcttgtgtg ggtaccaatg ctaccttctc tgtagtagca
    15241 acaggtacat accaatggca attaaataca ggttccggct ggaatgatat ttccggtgcc
    15301 acttcagcct cttatactgt taacggtgtt actttaacac agaatggata cacttaccgt
    15361 tgtgttgtaa ccggtcagtg tggtagtact aactcaaatg tggtaacctt aacagttaat
    15421 gcattaccgg caattactgc tcagccacaa agcctgaatc tttgttctgg cagtgctgct
    15481 tccttcagcg taacggctac cggagctggt ctttcttacc agtgggagtt gagtacaaat
    15541 ggtggcggaa catacacccc gattaccggt gctacttcag ctacttataa tattgcagct
    15601 gtcgctgtta ctgacaataa taatatgtat cgttgtgtag taacaggtac ctgtgcacct
    15661 gctgccactt cttcagcggc tacattactg gtgtcttcat caataactgt tactaccaat
    15721 cctgtcagcc agacagtttg tgaaggaaca aatctttcat tcacagtcgc agccggtggt
    15781 tctggtttat cttaccagtg ggaagtaagc accaatggtg gtacaagctg gagcaatatt
    15841 gcaaacggtg gtgtgtatac cggtgcaact actcctacgt tgaacattac gggagtgcct
    15901 ccaacattta atacttacag gtacagggct acagtatcca atgctgcttg tacaccggga
    15961 gtatctactg ttgctacgct gacagtaaat acattccctg taataactgc acaaccacag
    16021 aatgcaacca tttgtgaagg tgccagccaa agtttcagtg taactgcaac aacaggtgtt
    16081 ggtacactca gttatcaatg gcagctaagt gttaatggcg gtactagctg gactaacctt
    16141 gcaggtgcta ccagctccac ttttgctcaa actgctatac ctgccggaca aaacagctac
    16201 aggttcaggg tgattgtaac tgcaggttgc ggttctgtta cttccaatgc agccgtgtta
    16261 acagtaaata catatcctgt aatttcattt acagcgcctg ctgtaacctg tgtgtctgat
    16321 gcttccttta cattaagtgc aggtccggtt ggcggcgtgt tcagcggacc aggtgttgga
    16381 ggaacaacct ttaatccttc agcagcgggt gttggaccga agacggtgac ttacacagct
    16441 actaataatg gttgccagtc ttcaattggc agggtgatcc tggtaaatga atgtgctgaa
    16501 cgtcatctga cactggatca gtatcctgct atccaggttt tcccaagccc gaataacgga
    16561 cgcttcagca tcaggcttaa tactgacctc tacactaaac tgaatattaa aatattcaac
    16621 agtgccggtc agatggtcag atcccagatt gctaacggcc tcacttttgg aagtgtaata
    16681 ccgttggata tcactaacca ggcaagcgga acctaccatt tgttcctgtc caatgatgaa
    16741 aatggttcta tctcaaccaa agggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnncgcctt cgacaggctc aggctgacaa cttcggcaat ctctggctga caaattgggc
    16921 aagttcagat tgacaacttc tacaagtcct ggctgatatg acttttcgaa aagagacagt
    16981 acaagattct aattcattgc tgttatcagg cttagttatc gtaccttcgc ggcttcaaat
    17041 tttacgaaaa atgatcagtg taaaagacct gaaattagct tacggtaaaa gggttttgtt
    17101 cgaagaagtg aaccttaatt tcacaaaagg gaattgttat ggtgtcatcg gtgccaatgg
    17161 tgctggcaaa agtacttttc taaagatcct gagcggcgaa atagaaccta acaaaggaac
    17221 ggtggagatc acaccgggag aaagaatggc ggtactgaaa cagaaccagt tccagtttga
    17281 tgaggaaaca gtgctgaaca ccgtgatgat gggacataca aagttgtggg ctattgccaa
    17341 agaaagagaa acgatctatg cgctggctga ttttacagaa gaagatggca tgagggccgg
    17401 tgaactggaa catgagtttg gtgaaatggg tggttatacc gctgacagtg atgccgccac
    17461 tttactgagt gaactgggcg taaaagaaga atatcaccag tcactaatga aagatattcc
    17521 atccaacctg aaagtaaggg tgttattggc gcaggccata tttggtaatc ctgatatcct
    17581 cttattggat gagcctacca acggtctcga tattgaaacc attggctggc tggaaaattt
    17641 tttggcggac tacgaaaata ttgttctcgt ggtgagccac gaccgtcact tcctggatgc
    17701 agtatgtacc catgtggcag acgtggacag gcaaaagatc aagatcttta ccggtaacta
    17761 tactttctgg tatgagtcat cacaactgat ggcaagacag atcaatgaca agaacaagaa
    17821 aacagaagag aaaagacagg cgttgatcga cttcattgcc cgcttcagtg ccaatgcctc
    17881 aaaaagtaaa caggctacct caagaaagaa agcgctggag aaattatcga tagaagaaat
    17941 agaaccgagt tacagaaaat accccggtat catttttcag caattacggg aagtgggtaa
    18001 ccagatcctg aatgtagaca aactgaaaaa atctgttgat ggcagggtgc tgttcagtga
    18061 cgtaaccttt acagtgaaca aaggcgataa gatcgctttg ctgagccgtg acccgctggc
    18121 agttaccagt ttctttaata tcattaccgg cgatacaaaa acggacagcg gttcttatga
    18181 atggggcact actgttaccc atgcttacct gccacaggag aataaccatt tttttaatgg
    18241 aaaggataac ctgatggact ggttacgtca atatgtaccg gaccatgtaa aagatgtgga
    18301 tgaaccattc ctgcggggat tccttggtaa aatgttattc agcggtgatg atgtgctgaa
    18361 gacgaccaat gtactaagcg gaggggagaa ggtacgctgt atgctgagta agatgatgct
    18421 ccagagcccc aatgttattt tactggacca gcctactaac cacctcgacc tggaaagtat
    18481 acagagtttc aacgaacaat gtaccgatta caaaggcatc gtattattaa cgagccatga
    18541 ccatacattt atgcaaactg ttgccaacag ggtcattgaa ctgacaccta aaggcatcat
    18601 tgaccgcctg atgaccttcg atgaatacct cgttgatgaa agggtgaag
//
LOCUS       DAORTI010000065        17803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2824_length_17803_cov_7.955826, whole genome shotgun sequence.
ACCESSION   DAORTI010000065 DAORTI010000000
VERSION     DAORTI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17803
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_17803_cov_7.955826"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLO70_09530"
     CDS             <1..298
                     /locus_tag="PLO70_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQZ74749.1"
                     /translation="NSVIMDNVLLGDESIVGALTLIKEGEIIPARSVVVGNPGKIIKQ
                     VSDEMITWKTEGTKLYQQLPGDCFTMLKPCNPKRKAGKKHAPGNTGYSSWKSLK"
     gene            555..>2016
                     /locus_tag="PLO70_09535"
     CDS             555..>2016
                     /locus_tag="PLO70_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74750.1"
                     /translation="MKTFTFVKGIVPLLTLALFAVSTSAQTVTLNPSAAQNISTGGTV
                     NFTATRSSNSQTWPGGNGQFTYTWSSNPAGVTFTNNPNTTSNSSSGTVATFPSNGTYQ
                     ITCFVQEGGGLNATSAATTVNVTAPLPANLWATSSNGTQISAFTVSNGTYQSGPNNIF
                     PASFPGTTTGGTSTAALGRNASPSIALGHFYWLPNTGNNNGVVEVFAATATGANVTRI
                     GSIDFNGGSGSDLGFVRLGMGPDGTGWILAGDGTNLYLAKFTSNGVNPVAITVEDASV
                     TLSGGSVSTFQNGDLCISGAGNIYALANNGSGVTQIFIGFPNGSATTLTKKWDLVTPA
                     NTPFTGSVNGVAFDLLGSIYISTGDGLYYIDQNTANGPAGTVQCSLVRSQTGLQDLAS
                     NAFPSQSTLPVTLLNFSAAYRNGATTLNWETENEVNFSHYEVERKTATGNVYAEIGSK
                     TALGNTGRSSYQHIDNINSLTDDVVYYRLKMVDIDGG"
     assembly_gap    2017..2116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2374..3633
                     /locus_tag="PLO70_09540"
     CDS             2374..3633
                     /locus_tag="PLO70_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ74751.1"
                     /translation="MRRSLPLLSLCAATVLHSSISVNAQQADRFAYAVTDAQQMGANW
                     SFLRKLNLQTGEYSQVLLSGNDASFLAYNATTKKQLEAPLIDSRYGHTMNAAFGSGVA
                     AVAYDKKNNRLYYTPMLFDQLRYIDLKSMKVFYVTDKAFTGKPIKSADQGNIVTRMAI
                     AADGNGYALTNDGSQLIRFTTGKNLKIEDLGALVDDPANKGVSVHNSCTSFGGDMIAD
                     DDGNLFVISARNNVFKIDIGSKVATHLGTITGLPQGFTVNGAAVNENNQIIVSSAMQT
                     VNFTVDHKTWVAAPYTVTGTAWNSSDLANSNLLATAAKNKNAVTEVMSRNTPANSGDG
                     KVSIYPNPVSNNQFVIQFNQLEAGNYSVQVTDVMGRQVIQQLVSLSGDNHAQTIRLHP
                     SSAKGIYLIKVTDPNSRSVFNTKVVVQ"
     gene            complement(3678..4067)
                     /locus_tag="PLO70_09545"
     CDS             complement(3678..4067)
                     /locus_tag="PLO70_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74752.1"
                     /translation="MKYLLILLVFPFLSASECGKKKEKENVTDVRNDINCNDSIPVCV
                     RRLIDSANKEIPPNPPQQVDEYLYNGKKVFLFTAPCCDFFNMVYDDSCKAVCAPSGGI
                     TGKGDGKCEDFATTAKFVKSIWKNAEK"
     gene            4164..5165
                     /gene="obgE"
                     /locus_tag="PLO70_09550"
     CDS             4164..5165
                     /gene="obgE"
                     /locus_tag="PLO70_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQZ74753.1"
                     /translation="MEKSNFVDQIRIFCRSGHGGAGNKHFMRNKFTALGGPDGGDGGR
                     GAHIILKGNSNLWTLLHLRYYKNVLAEDGEKGGANNCHGRDGKDIIIDVPLGTIARDE
                     ETGKQEVEILEDGQEVIWMPGGKGGLGNSHFATPTNQAPEYAQPGLPGIEGWKVLELK
                     VLADVGLVGFPNAGKSTLLSVITAAKPKIANYAFTTLTPNLGMVEYRDGKSFCIADLP
                     GIIEGAAEGKGLGHRFLRHIERNSVLLFLIPADCDDHKKEFDILVNELEQYNPELLHK
                     QFIIAISKSDMLDVELKDAIEKELPDNIPHVFISSITNKGLTELKDLLWNVLNQPVE"
     gene            5490..6095
                     /locus_tag="PLO70_09555"
     CDS             5490..6095
                     /locus_tag="PLO70_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74754.1"
                     /translation="MKRSGKKIQPISKISLYSAQILYRKVRFTKLRPARSAPTEKSPV
                     AEPEMMDNRHNLSAAGNRWSSILRTLINILLIIVVIAGLGLYNDKNNYLVSAGVRQGR
                     NTVYELERIDSAANAIEEIFAASDTNQLAKILSPVTLEKRRPYFPQLIEFMPSFAADF
                     KTRKLQLVRARYAVYEFKSLRGKFLAEFCTDSNGCWVLMNF"
     gene            6106..8181
                     /locus_tag="PLO70_09560"
     CDS             6106..8181
                     /locus_tag="PLO70_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74755.1"
                     /translation="MKTLLYFRLFLSVIFSLLLIQFFSNYGNKAVHPDLNAMILGAFL
                     KQNSTPTQAEPFFKNYTFLFLSGTTLKGTAVIKDGYFSPNDIAAAGLGYGYSEEGPAE
                     KTVKGWITHGGYSADVPEVPASLRHFYDPTKPAGNRYLTDIANSKIMGSLQKYVLTNP
                     KTDAVEWAMGKPGIEALSVQDHFYTWERGKNWIRMALNEQNKKKQNEYMANAWRSLGE
                     TLHMIADNGCPPHVRNDAHPSPFLNNNTFLGNPDPYEEFMDQLKQEKPDVFAQFGKGT
                     PDPDFRNKINSLKTGKEIAHEMAYFTNKNFVTNETISGIDKNGKKIQQLIHPESPYSS
                     PLLQQMKYYESDHTYRTSSGVKQCADVVYFSDLIPRISEPTVTMECVISQAKALIPNI
                     IVAGKNMIKLFIPKLKVEIQSAENTTVKGMVKHMRDPEYATEIMYNGPVKLVLKDKNF
                     RIKKEIEITAKDGVFETSEFALGSSGNKVTAIIEFGGILINSDPFNCTVVSNTPVIKS
                     VAGGPTIDMLIYVNGTFDKDFRCGAYNKPAVTWIDIENNGIPVNKGLGEFKGTKKWGV
                     DIVEVSGIISEEKIIELRYRGTYTDDERQQQWDITVKDIPLLDPDQKGKHGQITYQLR
                     PYEMGLEKKVVNMYEHLTNITVKEVCIKNKQFSRNLSWTDWDRKFAGTTVASSPLMQQ
                     SAIQIRVNR"
     gene            complement(8397..9746)
                     /locus_tag="PLO70_09565"
     CDS             complement(8397..9746)
                     /locus_tag="PLO70_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HQZ74756.1"
                     /translation="MNFRNGLICLLFLVLIVNSCSKKDSGTPTPPPTTVPGCASAVTP
                     AAGSFVVTTSITLSWNAVSGATGYDIYLGTTANPTTTIVTNVTGTSYNYTIPATPNAT
                     YYWYALPKNSGGSATGCTAAISSFTYIVIQPPVPFGYYVVGYMPSYRNVTTIPDVKFR
                     MTNVVVYAFYGVNTSGTLSAPSSPTSSLAAVATKARANNARIFLGINDGSGDGKTNFK
                     NMAATPAGRTNFIKEVMNVVRQNNLDGIDMDWEFPTTTDGTDLTFTALMKELSDSLHR
                     DGRYYLSCAVTAGKYAGGIRDAIRNELFTYVDFFNIMAYDDFSTSAPYRHHSDYALAQ
                     TCLNYWLTTRGMPAAKAVLGLPAYGRPSGITQSGTILTYSGILGQGGNPQYDSAVVTS
                     GSFVNYTIYYNGQYTIKRKAKLAKDIANGVMLWEKGQDVHDANSLLKAACDTVGRAY"
     gene            9841..12006
                     /locus_tag="PLO70_09570"
     CDS             9841..12006
                     /locus_tag="PLO70_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQZ74757.1"
                     /translation="MKKFLVTIVLSLSLLAKTMADEGMWLPLLLGQQVYNDMVKKGLK
                     LTKEQLYNINKPSLKDAIIIFGGGCTGEIVSSQGLIFTNHHCGYNAIASASTVEKNYL
                     KDGFYARNKSEEIPTTLSVQFLLRIDDVTKEVMDSLKGLSGAERLQKQGAVLAAINKR
                     LSDAAQNIETRVSALFKGNQFLAFVYQRYTDVRLVGTPPESVGKFGGDTDNWEWPRHT
                     GDFSVFRVYTSADGKPAKYTDNNVPMKPKWYLPVSLRPLKDGDFSMIWGYPGGTNRYE
                     SSYGIKLATDINNPTLVNLRDVRLKYMFDEMKKDPAVKLQLASSYAGIANYWKFFDGE
                     TKQLLKYDVFGQKQKAEEKFNAWAKGKPEYENLFADWGKAYEAWRPYAEHRQYINEGI
                     AGSPLISFAGSLLQVEAAMVKTGSTAADVKKAVEAASNQRANFLKDENKVSDQNIVAS
                     ITRMFYENVNKEQHPVGFYGTLKNSYGPLDDDATYKKYAADIFSKTMIFDDAKWNAFV
                     ANPDAVTLQSDPAFAYASSFFTNYTSKYSSFFQQFNAKNAEYGRLYLKGIMEMDPVKA
                     KMMYPDATFTMRVSYGNVKSYRPRDAVFYDYVTTSKGLLEKYKAGDYEFDLPANQIEL
                     LKKKDFGQYMDPTRKDLVIGFITTNDITGGNSGSPVINGRGELIGLAFDGNYEALSHK
                     LAFDKDLNRTICVDIRYVLWCIDKLGGGTNIIKELKLMK"
     gene            12191..12637
                     /gene="tnpA"
                     /locus_tag="PLO70_09575"
     CDS             12191..12637
                     /gene="tnpA"
                     /locus_tag="PLO70_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQZ74758.1"
                     /translation="MSSYRQVYYHIIFGTKNRESTIAPSHEDELYKYIAGIIKNHQCK
                     LYRINGMPDHIHIFTDLHPSVCLSDLVKNIKVASSIWLKANGNFPSFTGWQEGYGAFT
                     YAEKDSNMIINYIRNQKEHHRTESFFEEYKRLLAEQKIEFDEKYLL"
     assembly_gap    12842..12941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13758..14402
                     /locus_tag="PLO70_09580"
     CDS             13758..14402
                     /locus_tag="PLO70_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74759.1"
                     /translation="MTILAVSTSIIHSQNLRFSLATDLGLQRNFQKEQQYWAIGHAVN
                     ANFHITPKEGIQVSFSYFSNGKFNNAVTATAKDVSTTPQRINYTNNARMRFRLLSIGY
                     KKYITGNCETEKGWNLYGAAGFGLLMGRVINTHSVAIDTSIYHVPVRSGEANFKRLTI
                     DLGLGWEKPLGGDFYFYTEARTFIPASDYPSKYIFVNKNAPFTGSLHAGLRIIF"
     gene            complement(14557..15090)
                     /locus_tag="PLO70_09585"
     CDS             complement(14557..15090)
                     /locus_tag="PLO70_09585"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HQZ74760.1"
                     /translation="MKNCLPFLLVLFLFTACKKEITELPPATQTGANTFGAKVNGEFW
                     IPQGFGPLPANDILEARMAGPDLTINARNFSSSPTETEFLLQVKNINGTGTYLLNTSV
                     GHPSSGSSYAYYVKRRLTPQNEWITSATHTGAVTITRIDTVARIVSGSFQFNALNMFN
                     APEPITVTEGRFDLQIQ"
     gene            15204..16319
                     /locus_tag="PLO70_09590"
     CDS             15204..16319
                     /locus_tag="PLO70_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQZ74761.1"
                     /translation="MRNTLTIRQIELYKLSIPLIEPFITSLGQDDNAENIVVKIITKE
                     GITGFGECSPYMPINGESQDTCFIVGQYFAKALIGKDPLDIEGCINLMDAIIYTNNSI
                     KSAFDIALYDIASQQAGLPLYQFIGGENNKTIITDYTVSIGEPGKMAADAVKIKAQGY
                     PAIKVKLGKNGKKDVERMKAIRAAVGNEIPLRIDANQGWTVEEAIETLYALAPLNIQH
                     CEEPIARWNFMQLPAVKRESPIPIMADECCGDDHDAARLIELNACDYFNIKLGKSGGI
                     FKALKMVSMAEQVNIHLQVGAMIESRLAMTAFAHFALCSPNIVHFDFDTALMLKEDPV
                     TGGIMYEKNGVVKVPEAVGLGATINESWLQKMEKVVI"
     gene            16379..17638
                     /locus_tag="PLO70_09595"
     CDS             16379..17638
                     /locus_tag="PLO70_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQZ74762.1"
                     /translation="MNVVVLKPLQHRGNECIGIYFEKSPRLNGAIQKYAGARWSQTNK
                     CWYVPLSKENYNKLAVALKGLATIEQSALHQYLADKKNKKQADKIRPVTIIKRSDAAK
                     TTVVPVSNQGNPVWINKRDFVYKKEKIGGVNSHIIPAMHQRLKLKAYSPSTIKTYLNE
                     MSQLLSLLKDIPADQLTPGHLKRYLVFCYEKLNLKENTLHSRINAMKFYYEQVLGREK
                     FFWEIPRPKKHLILPKILNEAELGRLFKALPNLKHKAMLFTVYSAGLRVSELAALKIK
                     DIDSGRMQIFIERAKGKKDRYVNLSPVLLDILRAYMRKHKPRPVEFLFESEQTRTAYP
                     LRTIQKIFEDAKNKAGIDKKLGIHSLRHSFATHLLEKGTDIRYIKDILGHFDIKTTER
                     YLHVSKKDLVNIISPLDDLWRKGQLEW"
BASE COUNT         5492 a         3608 c         3731 g         4772 t
ORIGIN      
        1 gaatagtgtg ataatggata acgtactatt aggtgatgaa agtatagtgg gggcattgac
       61 gttaataaaa gaaggagaaa taattcctgc cagaagtgtg gtggtaggga atcccggcaa
      121 gatcatcaaa caggttagtg atgaaatgat aacctggaaa accgagggca caaaattgta
      181 tcagcaatta cccggggatt gttttacgat gctgaaaccg tgtaacccta agcgtaaggc
      241 tggaaaaaaa catgcaccag gcaatactgg ctattccagc tggaaaagcc tcaaatagta
      301 aaagccttat atataatagc gaatacttgc agaaataatt ttagcagaat gcttgcaaaa
      361 tttgtataag aatactattt ttgtcttctc caacgttcaa ttccttctaa agaacccttg
      421 agcattacta tttatgctgt ccattactcc tttctgaatt ccctgatctc aaatcctgta
      481 aatgactacc tgagtccagc tccgttcaaa ccaatttgaa gaaggatttt tatttctaac
      541 ataaaaccat gagcatgaaa acttttactt tcgtaaaagg gatagtaccc cttttaacat
      601 tagccctatt cgctgtaagc acttcagcgc aaacagtaac tttaaatcct tctgctgcac
      661 agaatatcag cacaggagga acagtaaact tcaccgcaac aagaagcagt aattcccaaa
      721 catggcctgg tggtaatggt caatttactt acacctggag ttcaaaccct gctggtgtta
      781 cttttacaaa taaccctaac accaccagta atagcagttc cggtacagtt gctacattcc
      841 cttcaaacgg aacttaccag atcacctgtt ttgttcagga aggtggcggt ttaaacgcta
      901 cttctgcagc aactactgtt aatgtaacag caccgctccc tgccaatctt tgggccactt
      961 catcaaatgg tacacagatc tctgctttca cagtatcaaa tggcacctac cagagcgggc
     1021 caaacaatat ttttcctgcc agtttccccg gcacaacaac cggtggaaca tccacagctg
     1081 ctttaggcag aaatgcttca cccagcatag cactcggaca tttttactgg ctacccaata
     1141 ccggcaataa caatggcgtt gttgaggttt ttgcggccac tgcaaccggt gcaaatgtta
     1201 cccgtattgg ttccattgat ttcaatggtg gcagcggtag tgatcttgga tttgttcgtc
     1261 ttggtatggg acctgatggc acgggatgga tcctggctgg tgatggcacc aacctttacc
     1321 tggcaaaatt tacttcaaat ggtgtcaatc cagttgccat tactgttgaa gatgccagtg
     1381 tgaccctaag cggtggttct gtatctacat ttcagaatgg tgacttatgc atatcaggtg
     1441 ccggcaatat ttatgcattg gccaataacg gtagtggtgt aacacaaatt tttattggct
     1501 tccccaatgg cagcgctact accctgacaa aaaaatggga cctcgttaca cctgccaata
     1561 ctccctttac aggaagtgtg aatggcgtgg catttgactt gcttggctct atctacatct
     1621 ctacagggga tggtttgtac tatatagacc aaaacacagc taacggacct gcgggaacag
     1681 ttcaatgttc actggtcaga tcccagacag gattgcagga cctggcatct aacgccttcc
     1741 caagccagtc aacactacct gttactttgc tcaatttcag tgctgcatac cgcaatggtg
     1801 caacaaccct taactgggaa acagaaaatg aagtaaactt cagtcactat gaagtagaaa
     1861 gaaaaacagc gaccggaaat gtatatgctg aaataggcag caagacagcc ctgggaaata
     1921 ccggccgttc ttcttatcag cacatcgata atataaacag cctaacagat gatgttgttt
     1981 attatcgcct caaaatggtg gatatagatg gcggatnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnaata agttacagcc tggtatatat ataattcaaa tgtcaaccgg
     2161 agaagaacta tcagcgatta agttttctgt agttcgttga gttgaaaatc aatgctcagg
     2221 gttttaatac cttgaacccc ccttgattcg ttcggggggg gttttttatt tcacccatca
     2281 ccctgtgccc ctcataaaaa ttgtttttgt cacaaaaaga attgttgtaa tttaaagttg
     2341 ttcttttttt taaataacaa aaaaaccttg tgtatgagaa gaagtctacc cctcttatcc
     2401 ctttgtgcag caactgtttt acattcctct atttctgtaa atgcccagca ggctgatcgt
     2461 tttgcctatg cagtaacaga tgcccagcaa atgggtgcca actggagttt tttaagaaaa
     2521 ctgaacctgc aaacaggtga gtacagccag gtcctgctca gtggcaatga tgcatcattt
     2581 cttgcatata atgcaacaac aaaaaaacaa ttagaagccc cgctcatcga ttcccgttat
     2641 ggacatacta tgaatgcggc gtttggcagt ggggtggcag cagttgcgta tgacaaaaag
     2701 aacaatcgtc tttattatac accaatgctt ttcgatcagt tgcgttatat cgatttaaaa
     2761 agcatgaaag ttttttatgt aactgataaa gcatttaccg gaaaaccaat taagtcagct
     2821 gaccagggca atattgtaac ccgtatggct attgcagcag atggaaacgg gtatgcattg
     2881 accaatgatg gatctcagtt gatccgcttt accacaggca aaaacctgaa gattgaagat
     2941 ctcggcgcat tggtagatga tccggccaac aaaggcgttt ccgtacataa ttcctgcact
     3001 agttttggtg gcgatatgat cgcagacgac gatggaaacc tgtttgtcat ttctgcccgc
     3061 aacaatgttt tcaaaataga tattggatca aaagtggcta cacatcttgg taccatcact
     3121 ggattacccc agggttttac tgtaaacgga gcagctgtta atgaaaacaa ccagatcata
     3181 gtaagcagtg ccatgcaaac agttaatttc actgttgatc ataaaacatg ggtcgctgct
     3241 ccatatactg ttaccggtac tgcctggaac agttccgatc tggccaatag caacctgctt
     3301 gctactgctg ctaaaaacaa aaatgccgtg acagaagtta tgtcacgcaa taccccagca
     3361 aattcaggtg atggcaaggt tagcatttat ccaaaccccg tttccaataa ccagtttgtg
     3421 attcagttca atcaactgga agccggtaat tattcggtac aggtaactga tgtgatggga
     3481 cgccaggtta tacagcaatt agtaagcctg agcggagata atcatgcaca aacgatcagg
     3541 ctccatcctt cctctgccaa aggtatttat ttgataaaag taactgaccc caacagcaga
     3601 tccgtattca atacaaaggt ggtggttcag taaaaaatag tttcttaatt aaaaggcctg
     3661 cttatagcag gtctttttta tttctcagca tttttccaga tcgactttac gaatttggct
     3721 gttgtggcaa agtcttcgca tttaccatcc cctttcccgg tgatcccacc cgaaggtgca
     3781 cacactgcct tacaactatc atcatatacc atattgaaaa aatcacagca tggtgctgta
     3841 aacaaaaaaa ctttttttcc attataaaga tattcatcta cctgctgggg aggattgggc
     3901 ggaatctcct tattcgcact atcaatcaac ctccttacac aaacaggaat agaatcatta
     3961 caattgatgt cattccttac atctgttaca ttttctttct cttttttttt gccacactca
     4021 ctggcagata agaaagggaa aaccagcagg atcaacagat atttcataaa tgagctgtta
     4081 ggattggtta gggtatattt gcatgaaccc accgaaggta agaggaaaga tacaaaggcg
     4141 ggattcacat taaaaataca gttttggaga aaagtaattt tgtagaccag atccgcattt
     4201 tttgccgcag tggacatggt ggcgctggta acaagcattt tatgcgcaat aaattcaccg
     4261 cattgggtgg tcctgatggc ggtgatggtg gccgtggcgc ccatatcatc ctgaaaggca
     4321 atagtaatct ctggacctta ctgcacttac ggtattataa gaatgtgctg gccgaagatg
     4381 gagaaaaagg tggcgccaat aactgccatg gccgggatgg aaaagatatc attattgatg
     4441 taccgctggg tactattgcc cgggatgagg aaactggcaa acaggaggta gaaatactgg
     4501 aagatggaca agaggtaatc tggatgcccg gtggtaaagg aggattgggc aactcacatt
     4561 ttgccacacc taccaaccag gcacctgaat atgcgcaacc tggattgccg ggtattgaag
     4621 gctggaaagt attggaatta aaagtactgg cagatgtagg tcttgttggt tttcccaatg
     4681 caggcaaatc aactttatta tctgtcatca ctgctgccaa acctaagatt gccaattatg
     4741 cttttacaac cctcactccc aatttgggca tggtggaata cagggatgga aaaagttttt
     4801 gcatagccga ccttccggga attattgaag gtgcagcaga aggcaaagga ttaggtcatc
     4861 gttttctccg gcatatagaa cgcaactctg ttttattgtt tttaattccc gcagactgtg
     4921 atgatcataa aaaagaattt gacatattgg tgaatgagct ggaacaatac aaccccgagt
     4981 tgctgcataa acaattcatc attgccatca gcaaaagcga tatgctggac gttgaattaa
     5041 aagatgctat tgaaaaagaa ttgccggata atattccaca tgtgtttatc tcgtcaataa
     5101 ccaataaagg attgacagaa ctgaaagacc ttttatggaa tgtactgaac caaccggtgg
     5161 aataagaagt atgctacaaa tgaattaagt ggtaatacct gaattggttg agaatatgac
     5221 atatcttttt tctatttcag aaagaagtat gctgctatct ggttcaagac tccgatgatc
     5281 gtaaaattat taaggattca attacaccct gtttagaaaa cagtttgcca gaaaaaaagc
     5341 tggattgaaa gttcattatt ttagaaacgc ttctccattt tatgagtaag cccttgtgta
     5401 ctaagatttg ttatccggga gtatgaccga tatcattgaa aatgatgtag aaatggccca
     5461 acttgcgata agattttcca attgggttta tgaaaaggtc aggcaaaaaa atccagccaa
     5521 tttcaaaaat tagtctttat tctgcccaaa ttttatatcg aaaagtccga ttcacaaagc
     5581 ttagaccagc tcgttccgcc cccacagaaa aaagcccggt agctgaacca gaaatgatgg
     5641 acaaccgcca taatttatca gctgccggaa atcgatggtc aagcatatta agaaccctaa
     5701 ttaacatttt gctgatcata gtagtaatag ccgggcttgg attatataat gataaaaata
     5761 attatttagt ctcggcaggc gtcaggcaag gccgtaatac agtttatgaa cttgaaagaa
     5821 tcgatagtgc agcaaatgca atagaagaga tttttgctgc atcagataca aaccaattag
     5881 cgaaaatttt gtcaccagtt acattagaaa agcgaagacc ttacttcccg cagttaatag
     5941 aattcatgcc gtcattcgct gcagatttca aaacaagaaa gctacagctg gtgcgggcca
     6001 ggtatgcagt ttacgagttc aaaagtttga ggggaaaatt tcttgctgag ttttgtaccg
     6061 atagcaacgg atgttgggta ttgatgaact tttaaaacaa acaaaatgaa aacacttctt
     6121 tatttccgtt tgttcttaag tgtaatattt agtttattac taatacaatt tttctccaat
     6181 tatggcaaca aagcagttca tcctgacctg aatgccatga tactgggtgc atttctaaag
     6241 caaaacagca ctcccacaca agcggagccc ttttttaaaa attatacatt tctgttcctt
     6301 tcaggaacaa cactcaaggg tactgcagtt atcaaggacg gatattttag tcctaatgat
     6361 attgccgctg cgggcctggg atatggatat tctgaggaag gaccggctga aaaaacggtg
     6421 aaaggctgga ttacacatgg aggatattca gctgatgttc ctgaagtacc tgcttcttta
     6481 aggcactttt atgacccgac caaaccagcc ggcaatcgtt atcttactga tattgccaat
     6541 tcaaaaatta tggggtcttt gcaaaaatat gttctcacca accctaaaac cgatgctgta
     6601 gaatgggcta tgggtaagcc aggtatagaa gctctcagtg tccaggatca tttctataca
     6661 tgggaaaggg gaaagaactg gataagaatg gcactaaatg agcaaaataa aaaaaagcaa
     6721 aatgaataca tggccaatgc ctggcgatct cttggagaaa ccttacacat gattgctgat
     6781 aatggttgtc cgcctcatgt tcgcaacgat gcacaccctt cacctttctt aaacaacaat
     6841 acatttttgg gcaacccaga tccctatgaa gaatttatgg atcaactaaa acaagaaaag
     6901 ccagatgttt ttgcacaatt tggcaagggt acaccggatc cggattttcg gaataaaatt
     6961 aattcattga aaacaggaaa ggaaatagcc catgaaatgg cgtatttcac caataaaaat
     7021 tttgtaacca atgaaacaat ttccggtata gataaaaatg gtaaaaaaat acaacagctc
     7081 attcatccgg aatcccctta ctcctctccg ctattgcaac aaatgaaata ttatgagtca
     7141 gatcatactt atcgtacttc ctcaggtgta aaacaatgtg cagatgttgt ctatttttca
     7201 gatcttatac cccgaatatc cgaaccaact gtaacaatgg aatgtgttat ctctcaggca
     7261 aaagccctta ttccaaatat cattgtagca ggcaaaaaca tgataaaact cttcattccc
     7321 aaactaaaag tggagataca atcagctgag aatacaacgg ttaagggcat ggtaaaacat
     7381 atgagagatc ccgaatatgc cacagagata atgtataatg gccccgtcaa actggtcctt
     7441 aaagacaaaa atttcagaat aaaaaaagaa atagagatta cagcaaaaga cggggttttt
     7501 gaaaccagtg aatttgcatt aggtagttcc ggcaataaag tgactgccat cattgaattt
     7561 ggaggaatac ttatcaattc agatccgttc aattgcactg tagtcagtaa tactccggtc
     7621 ataaaaagcg ttgccggagg ccccacaatt gatatgttga tttatgtaaa cggaaccttt
     7681 gacaaggatt tcagatgcgg agcttataac aaacccgcag taacgtggat agatattgaa
     7741 aacaatggta tccctgtcaa caaggggttg ggggaattca aaggaacaaa aaaatggggt
     7801 gttgatattg tagaggtatc aggaatcatc agtgaggaaa agattatcga gttgcgctat
     7861 cgcggcactt acacggatga tgaaagacaa caacaatggg atattacagt aaaagatatt
     7921 cctcttttgg atcccgatca aaaaggaaaa catggacaga taacttacca gcttcggcct
     7981 tacgaaatgg ggctcgaaaa aaaagttgtc aatatgtatg agcacctgac caatataact
     8041 gtaaaagaag tttgtataaa aaataaacag ttttcccgaa atttatcgtg gacagactgg
     8101 gacagaaaat ttgccggcac tacagttgcg tcaagtccgc tgatgcaaca atctgccatc
     8161 caaataagag tgaaccggta aattttaata aaagaagaag tcatattgta tctggtatgg
     8221 atcttttaca taagaggaag aaattcagat ttctgaaata atacgaggtt tttgcaacga
     8281 acttccggaa aaggcaatta aaaaaattac tcagccatat gcaattattg catttgattt
     8341 gtaaaaaaag ctgcggaaaa atgccggtgt cgataaaagt gcttcattag ccggcattaa
     8401 taggcccggc ccactgtgtc gcaggcagct ttgagcaagg agttggcatc atgtacatcc
     8461 tgtccttttt cccagagcat cactccgttg gcaatgtctt tggccagttt ggcctttcgt
     8521 tttatagtgt actgcccgtt atagtaaatc gtgtaattca caaaagaacc gctggtgacc
     8581 acggctgaat catattgcgg gtttccaccc tggcccagta ttccgctgta ggtaagtatg
     8641 gttccgcttt gcgtgatgcc cgaaggtctt ccgtaagccg gcaatccaag aactgctttt
     8701 gcagcaggca ttcccctcgt tgtcagccag taattcaaac aggtttgcgc cagcgcataa
     8761 tcactgtgat gccggtaagg cgctgatgta ctgaaatcat catatgccat tatgttgaag
     8821 aaatcaacat aagtaaataa ttcattgcgg atggcatctc ttataccacc tgcatattta
     8881 cctgcagtaa ctgcacaaga caaataataa cggccatccc tgtgcaaaga atcagataat
     8941 tctttcatta gggcggtgaa tgtcagatca gttccatcag tggtagtagg aaattcccaa
     9001 tccatatcga taccatccag gttattttgc ctcaccacat tcattacttc tttaataaaa
     9061 ttggtacgac cggcgggggt agccgccatg tttttaaaat ttgttttccc gtcaccgctt
     9121 ccgtcattga tacccagaaa gatcctggca ttattggccc tggcttttgt agccacagca
     9181 gccagcgatg aggttgggga ggaaggtgct gataaagtac cggaagtatt caccccataa
     9241 aatgcataga ccaccacatt cgtcatcctg aattttacat ccggtatggt agtcacattc
     9301 ctgtaagagg gcatgtagcc aaccacataa tacccaaaag gaaccggtgg ttgtataacg
     9361 atataagtga aagaactgat agcagccgta caacccgtag cagaaccgcc ggagttttta
     9421 ggcaatgcat accagtagta agtggcattg ggtgtggccg ggatcgtata gttatagctg
     9481 gtacccgtta cattggtaac gatagtggtt gttggattag ccgttgtacc aaggtatata
     9541 tcatagccgg ttgcaccgga tacagcattc caggaaaggg ttatactggt ggttaccaca
     9601 aaagagccgg cagccggtgt aaccgctgaa gcacaacccg gtaccgttgt gggcggaggt
     9661 gtaggggtgc cgctatcctt tttactacac gaattgacaa ttaaaactaa aaacagcaga
     9721 cagattagac cattcctgaa attcataagc agtaattcgg ccttcaaatt aatggattcg
     9781 gatgaaactc agcattttga tatgcctttt tctgtaggtt tgccctctaa aatttcaagt
     9841 atgaagaagt ttctggtaac gattgtttta tcgctttcac tcctcgccaa aacaatggct
     9901 gatgaaggca tgtggctgcc gttattgctc ggacagcagg tttataatga catggtgaaa
     9961 aagggattaa aactcaccaa agaacagtta tacaatatca acaaaccctc tttgaaagat
    10021 gcgatcatca ttttcggtgg aggttgtacc ggtgagatag taagctcaca gggtttgata
    10081 tttaccaatc accactgcgg atacaatgct attgcttctg ccagtacggt tgaaaaaaat
    10141 tacctgaagg atggctttta tgcacgcaat aaaagtgaag agatcccgac cactctttct
    10201 gttcagttcc tgttacgcat tgatgatgta acaaaagagg taatggattc attaaaagga
    10261 ttgagcggag ctgaaagatt gcaaaagcaa ggcgccgtat tggccgctat caataaaaga
    10321 ttgagtgatg ccgctcagaa tattgaaaca agggtaagtg ctttgtttaa aggcaaccag
    10381 ttccttgcat ttgtgtacca gcgttatact gacgttcgcc ttgtaggcac acctcctgaa
    10441 agtgtgggta agtttggggg tgatactgac aactgggaat ggccccgtca taccggtgat
    10501 ttttcagtgt ttcgtgtata tacatcagcc gatggcaaac ctgccaaata cactgataat
    10561 aatgtgccga tgaaaccgaa atggtattta cctgtttccc tgcgaccctt aaaagatggt
    10621 gatttctcca tgatctgggg ttacccgggt ggtactaacc gttatgaaag ttcctacggt
    10681 atcaagctcg ccaccgacat caacaatccc accctggtga atctccgtga tgtacgattg
    10741 aaatacatgt ttgatgaaat gaaaaaagat ccggcagtca aactgcagct ggcctcttcc
    10801 tatgcaggca ttgccaacta ttggaaattc tttgatggcg aaaccaaaca actgttgaaa
    10861 tatgatgtgt ttggtcaaaa gcaaaaagca gaagagaaat tcaatgcgtg ggcaaaagga
    10921 aaacctgaat atgaaaactt gtttgccgat tggggcaaag cctatgaagc atggagacct
    10981 tatgcagaac accgccagta tatcaatgaa ggtattgccg gttccccgtt aattagtttt
    11041 gcgggttctt tactgcaggt agaagctgcc atggtcaaaa ccggttcaac cgctgctgat
    11101 gtaaagaaag cagtggaagc tgccagcaac cagagagcca atttcctgaa agatgaaaac
    11161 aaggtatctg accaaaacat tgtggcttct attacaagaa tgttctacga gaatgtaaat
    11221 aaagaacagc acccggttgg tttttatggc accctgaaaa attcatacgg cccgctggat
    11281 gatgatgcta cctataaaaa atatgcagca gatattttca gcaagaccat gatcttcgat
    11341 gatgcaaaat ggaatgcttt tgtggccaac cctgatgcag ttacattaca gtcagaccct
    11401 gcctttgctt atgcaagctc ctttttcact aattatactt caaagtacag ttcattcttc
    11461 cagcagttca acgctaagaa tgcagaatat ggaagattgt acttgaaagg tattatggaa
    11521 atggatccgg taaaagccaa aatgatgtat cccgatgcta cattcactat gcgggtcagc
    11581 tatggtaatg taaaatcata tcgtccgaga gatgcggtat tttatgatta tgtgaccacc
    11641 tctaaaggat tgctggaaaa atacaaagca ggagattatg aatttgattt accggccaac
    11701 cagattgaat tgctgaagaa aaaagatttt ggccagtata tggatcctac ccgcaaagac
    11761 ctggtgatcg gtttcattac taccaatgat atcaccggtg gtaattccgg ttccccggtt
    11821 attaatggca ggggcgaact gatcgggtta gcttttgatg gcaactacga agcgctgagc
    11881 cataaactgg cttttgataa agacctgaac cgcactattt gtgtagacat ccgctatgtt
    11941 ttgtggtgta ttgataaatt aggaggcggc actaacatta ttaaagaact gaagttgatg
    12001 aaatgattct catctcctta acagcaaagc catttcgtaa taacgggatg gctttgctgt
    12061 ttataaacta tttataccac cattatccgg aaaacccaga agggcttaga tttgaataca
    12121 aaaaactata gccggtgaag taaaactaac ctactgacct tagcgaaata aaaaaacctt
    12181 atcctgtatt atgagttcct accgccaggt atactatcat attattttcg gaacaaaaaa
    12241 ccgggaatcc acaatcgccc cctcccatga agatgagtta tataaataca tagcaggaat
    12301 aatcaaaaat catcaatgca aactataccg gataaacggg atgcctgacc atattcatat
    12361 tttcacagac ctccatcctt ccgtttgcct gagtgacctt gtaaagaata taaaagtggc
    12421 cagtagtatc tggctgaaag cgaatggcaa ttttccgtca tttaccggat ggcaggaagg
    12481 atatggtgct ttcacctatg ccgaaaagga cagtaacatg atcatcaatt atatcaggaa
    12541 tcaaaaagaa catcacagaa cagaaagttt ctttgaagaa tacaaaagat tgctggctga
    12601 acaaaaaatt gaatttgatg aaaaatattt gctgtagtta ttcaacccct ccggggttgg
    12661 gcatccggca catacattta cccctgtttg caacaggggt tatacagctt taaccccttc
    12721 ggggttgggc atcctccaca ttcatcaccc ctgtttgtaa cagggtttat acagatttaa
    12781 cccctccggg gttgagacac tttgcattca ttagacttgt ttgtaataag ggctatacca
    12841 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcacata catttacccc
    12961 tgtttgcaac aggggttata cagctttaac cccttcgggg ttgagacact ttgcattcat
    13021 tagacttgtt tgtaataagg gctataccaa ttcaacccct acggggttgc gcatcggcac
    13081 atacatttac ccctgtttgc aacaggggtt atacagcttt aaccccttcg gggtttatcg
    13141 tcttcttcca atttggttta ttcttcatca aagagatatt ttaatcgaac actcttcaat
    13201 cagttcccgc cctaaattaa ttttgaaaac aaaagcccga agggcttgaa ccttattaac
    13261 caccggttat accggtgagc ataatcagca gttttcgatc aaccccaacg gggttgaatt
    13321 taattactct tttgtcttcg cttcacacca aaagatatat cattcaagta ctctataatc
    13381 aaaaccagct cttaataatt tttgaaaacc aaagcccgaa gggcttgaac atggctaacc
    13441 accggtataa ccggtggtct tcatcggcag ctttcgatca accccaacgg ggttgaatat
    13501 aattactctt ttgtcttcgc ttcacaccaa aagatatatc attatagtac tctataatcc
    13561 aaaccagccc ttaacaactt ttgaaaacta aagcccgaag ggcttgaacc tggttaacca
    13621 ccggtataac cggtggtctt catcggcagc tttcaatcaa ccctgaaggg gttgaatcta
    13681 tcattccctt ctgtccaaac aattatttca gtaacattgc aacaacgtta tcctgattgc
    13741 tgaaacgact taccatattg accatacttg ctgtaagcac cagcattatc cactcgcaaa
    13801 acctgcgttt cagccttgcc accgaccttg gcttacaacg taattttcag aaagaacaac
    13861 aatactgggc tattggccat gctgtcaatg ccaattttca cattactcca aaagaaggca
    13921 tccaggtgtc tttttcttat ttcagcaatg gaaaatttaa taatgcagta acggccaccg
    13981 ctaaagatgt atccactacc ccgcaacgca tcaattatac caataatgcc aggatgagat
    14041 tcaggctgct ttccattggt tataaaaaat acattacagg caattgcgaa acagaaaaag
    14101 gctggaacct ttatggcgcc gccggatttg gcctgcttat gggcagggtc attaatacgc
    14161 attcggttgc gattgatacc agcatctatc atgtgccagt acgcagcggc gaagccaatt
    14221 tcaaaaggct gacgattgac ctgggcctgg gctgggaaaa accgttgggc ggagacttct
    14281 atttttatac cgaagcaaga acattcattc ctgccagtga ttacccgagc aaatacattt
    14341 ttgtaaataa gaatgccccc tttaccggct ctttgcatgc aggacttcga attattttct
    14401 gatcatttcc cggcattaat aaaaacgctg aatgagagta ggtgcgggta gcaatcagag
    14461 tggtcacatt tcgataaact caatgagaca gtaaccttca ctttcccaaa aactcatcct
    14521 tagcatttgc tgactttaga gtagtaaaaa tcttatttac tgaatttgca gatcaaacct
    14581 cccttcggta accgtaatgg gttccggtgc attaaacata ttcagtgcat tgaactgaaa
    14641 gctgcccgaa acaatacggg ccaccgtatc tattcgggtg atggtaacag cgccggtatg
    14701 cgtggcagaa gtgatccatt cattctgtgg cgttaatctt ctttttacat agtatgcata
    14761 gcttgatccg cttgaagggt gcccgacaga tgtattgagt aagtaagtac cggttccgtt
    14821 aatatttttt acctgtagca aaaattctgt ttctgtgggc gaagaagaaa aattccgggc
    14881 attgatcgtt aaatccgggc ccgccattct tgcttccagg atatcattgg ctggaagagg
    14941 cccaaaaccc tgtggtatcc agaactcccc gttcactttg gcaccaaatg tattggcccc
    15001 tgtttgggtg gccggcggca attcggttat ttcttttttg caggccgtaa aaagaaaaag
    15061 gacaagaagg aacggaagac agtttttcat gttacaagat tatttatata acggaagcgg
    15121 gagaattttg gtatgcaggg caaaaacaaa gccagactaa cagcccaaac cttttatctt
    15181 tatcggcctt atacaactga tctatgcgca atacattaac gatccggcaa attgaattat
    15241 acaaattatc cattccgctt attgagccct ttatcacatc actcgggcag gatgataatg
    15301 cagaaaatat agtggtgaaa ataatcacca aagaaggcat tacaggtttt ggtgaatgca
    15361 gcccttatat gcctatcaat ggcgaaagcc aggatacctg ttttatcgtg ggacaatatt
    15421 ttgccaaagc gctgatcgga aaagatccgt tggatattga aggttgtatc aacctgatgg
    15481 atgccatcat ttacaccaat aacagcatca agagtgcctt tgatatcgcc ttgtatgata
    15541 ttgcttcaca gcaggcaggg ttgcctttgt accagtttat aggcggagaa aataacaaga
    15601 ccatcattac cgattataca gtgagtattg gtgaaccggg taagatggcg gccgatgcgg
    15661 tgaaaataaa agcacagggt tatcccgcca tcaaagtaaa gttgggaaaa aacggaaaga
    15721 aagatgtgga acggatgaaa gctatccgtg ctgccgtggg taacgagatc cctttacgca
    15781 tagatgccaa ccagggctgg acagtggaag aagccattga aacattatat gcacttgcgc
    15841 cgttgaatat acagcattgt gaagaaccca ttgcccgctg gaattttatg cagttgccgg
    15901 cagtaaaaag agaaagtcct atccccatca tggcggatga atgctgcggg gatgaccatg
    15961 acgcggcgag attgatagaa ctaaatgcct gtgattattt taatatcaaa ctcggtaaat
    16021 ccggcggcat ttttaaagcg ctgaagatgg tgagtatggc cgagcaggtc aatattcatt
    16081 tacaggtagg tgccatgatc gaatcacgat tagcgatgac ggcctttgca cattttgcat
    16141 tatgcagtcc caacatagta cattttgatt ttgacaccgc actcatgctt aaagaagacc
    16201 cggtgaccgg cggcatcatg tatgagaaga atggcgtggt gaaagtgccg gaagcagtag
    16261 gactcggagc cacgatcaat gagagctggc tgcaaaagat ggagaaagtg gtgatctgac
    16321 gggcagctgt tttacacttt aataagtttt agtacattca ttggacaaac cttatcaaat
    16381 gaatgtagta gtattaaaac cactgcaaca ccggggcaat gagtgcattg gtatttattt
    16441 tgaaaaatct ccccgattaa acggtgcgat acaaaaatat gccggggcca ggtggagcca
    16501 gaccaataaa tgctggtatg taccattaag taaagagaat tataacaaac ttgccgtggc
    16561 cctgaaagga ctggccacca tagaacaatc agccctgcat caatacctgg cggacaagaa
    16621 aaataaaaag caagcagata aaattcggcc tgttaccata attaaaagga gtgatgcggc
    16681 gaaaacaacc gttgtcccgg ttagcaacca gggaaacccg gtgtggatta ataaacggga
    16741 ttttgtatac aagaaagaaa agattggcgg tgtaaatagc catataatac ccgccatgca
    16801 ccagcgtctg aagctgaaag catatagtcc ttctactata aaaacgtatc tgaatgaaat
    16861 gtcgcaacta ctcagcctgc tgaaggatat acctgccgac cagcttacac ctggccacct
    16921 gaaaagatat cttgtgtttt gttatgaaaa attaaatctg aaagaaaaca cattgcatag
    16981 ccgtatcaat gcgatgaagt tttattacga acaggtgctg ggaagagaaa aatttttttg
    17041 ggagatacca cgacccaaaa aacacctcat tcttccaaaa atacttaatg aggccgagct
    17101 gggaagatta tttaaggcgt tacccaactt aaaacataag gctatgttgt ttacagtgta
    17161 cagtgccggc ctcagggtaa gtgagcttgc tgcgctaaaa ataaaagaca ttgacagcgg
    17221 caggatgcaa attttcatag aaagggctaa aggaaaaaaa gataggtatg taaatcttag
    17281 tcctgttttg cttgacatac tgagggcata tatgagaaag cacaaacccc gccctgttga
    17341 atttcttttt gaatcggaac aaacaagaac tgcctatccg ctcaggacca ttcagaaaat
    17401 atttgaagat gctaaaaaca aagcaggcat tgacaaaaaa cttggtatac attccttaag
    17461 gcacagcttt gccactcatt tattagaaaa agggactgat atcaggtata taaaagatat
    17521 acttgggcac tttgacatta agacaacaga aagatatctc catgtaagca aaaaagacct
    17581 tgtgaatatt ataagtccgc ttgatgacct atggagaaag ggacaattgg aatggtaaac
    17641 tgtgattttg tattgacgcc gtaacaatac aaaaagcgta ggactaaaac attggttttc
    17701 aatccgagta acttttgaaa tttgagattc tacggcgtat atttatgtgt tatcggcaat
    17761 gctacttaac catagagggg tgtttatcgg gggacaggga gga
//
LOCUS       DAORTI010000066        17749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2836_length_17749_cov_5.388267, whole genome shotgun sequence.
ACCESSION   DAORTI010000066 DAORTI010000000
VERSION     DAORTI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2836_length_17749_cov_5.388267"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1752)
                     /locus_tag="PLO70_09600"
     CDS             complement(328..1752)
                     /locus_tag="PLO70_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQZ74763.1"
                     /translation="MDINCTKEELELLKKVATAAETLGMEAYLIGGFVRDKILGRETK
                     DADFVCVGDAIDLAKEVAKKFRPQPHVDYFKNFGTAHFRLENEFDIEFVGARKESYRF
                     DSRKPEVVPGTVKDDQDRRDFTINALAISLNKENYGTLIDPFHGIKDIDDKIIRTPLA
                     PAQTFTDDPLRMMRAIRFATQLGFIIEEGTWQGIIDNAERIKIISQERITDELNKIVA
                     APRPSVGFDLLYKSGLLQLIFPQMVDLAGAEYKDGLGHKDNFYHTLQVLDNLSANSDD
                     LWLRWAAILHDIAKPATKRFEEGHGWTFHGHEVVGGRMVPKIFAKLKLPLNEKMRFVR
                     KMVELHLRPISLTKENITDSAIRRLLFDAGDDFDSLMLLCNADITSKNKQKVKRFLEN
                     FQLVRLRCKEVEEKDHVRNWQPPVSGEQIMEIFGLPPSRQVGVIKDALKDAMLDGVIP
                     NTYEAAHAFMLEKAKELGLKQVKK"
     gene            complement(1801..2370)
                     /locus_tag="PLO70_09605"
     CDS             complement(1801..2370)
                     /locus_tag="PLO70_09605"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQZ74764.1"
                     /translation="MVNFEKDIEQSLKVLHDGGLILYPTDTVWGIGCDATNEKAVERV
                     YRLKKRSDEKAMIVLVADERDVLKYVASPDLSVFDYLEGATRPTTVIYEDAIGLAENL
                     VGQDGSIAIRICDEPFCKHLLKRFRKPVVSTSANISGQPAARLFTDISTEIKEGVDYL
                     VNYRQDDTTVAAPSSVIRWQNGKVQVLRP"
     gene            complement(2364..3515)
                     /locus_tag="PLO70_09610"
     CDS             complement(2364..3515)
                     /locus_tag="PLO70_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQZ74765.1"
                     /translation="MNIGFDAKRAFHNGTGLGHYSRTLIRSLSDYHPEHQYYLFNPRH
                     SSEFELKGNNLQEIVPTDFLSRLFSSAWRSSWVKKDLQRLKIGLYHGLSHEIPVGIER
                     TGIKSVVTIHDLIHERYPEQYNAIDVKIYSKKFRYACEHADKVIAISEQTKRDIIEFY
                     KTPENKITVCYQSCNPAFGEEVNEEERKRIREEYDLPEQFFLYVGSVIERKNLLNICK
                     ALFLLRNELDIPLLVIGEGGKYKQQVKDFVTQNGLGSKVIFLSEHEKARSSPSFRSAK
                     VFPAIYQSAIAMIYPSFFEGFGIPVLEALWSRLPVITSSVSCLPEAGGSGAYYVNPAS
                     AEEIATGMKKIYRDKGLAATLKEKGWEHAQNFTQQKCADAVMNVYKSIW"
     gene            3641..4447
                     /locus_tag="PLO70_09615"
     CDS             3641..4447
                     /locus_tag="PLO70_09615"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQZ74766.1"
                     /translation="MKELILEAWGNRDLLNDKKYSDAVRAVIEEVDKGRLRVAFPADN
                     GWVVNEWVKQAILLYFGIQQMQTWDLAPFEFYDKMLLKKNYKELGVRAVPHAVARYGA
                     YLAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKAVHLSGGVGIGGVLEPLQA
                     SPVIIEDGCFIGSRCIVVEGVIVEKEAVLGANVVLTQSTKIIDVSGPEPVEMKGRVPA
                     RSVVIPGSYTKKFPAGEFGVGCALIIGQRKPSTDLKTSLNDALRDFNVSV"
     gene            4471..4554
                     /locus_tag="PLO70_09620"
     tRNA            4471..4554
                     /locus_tag="PLO70_09620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4505..4507,aa:Leu,seq:cag)
     assembly_gap    4713..4812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4963..8091
                     /locus_tag="PLO70_09625"
     CDS             4963..8091
                     /locus_tag="PLO70_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HQZ74767.1"
                     /translation="MRNLLLSATLLLCHYFVTAQQNFWQPAEESAIGKDLFTERQKPA
                     AYKIFQLNESAFATATGLAPSEKSIPASQSTSVIGIPNAEGRIENFRVVDAPVMDPLL
                     AARYPGIRSYAGVSVTDPSVTIRFSVSPQGVSAMILSASRPTIYIDPLSDNYYLVVSR
                     TDVSNYRQPFTCETMNAIAEVADTPASTLRNADDGRLRTYRLALVASGEFSQYWLNGT
                     ETTDAERKAKVLAAMNDKMTRVNGIYERDFGVRLLLIANNDAVIYLNAATDPIGTTSS
                     SWNTQTQTTCTNVIGSANYDVGHLVHRGPTNNGNAGCIGCVCKTNKGSGWTSYIDLSS
                     DYFVVDYLTHELGHQFGGNHTFTHANEGTIAQVEPGSGSTIMSYAGITGATTDVQSHN
                     DDYFHAITIQQVTDYIKSANGTCAVSTVTGNATPTANAGVDYVIPRSTPFVLSGTGSD
                     ADAGDVLTYCWEQNNPRATGSSTVPSATATAGPQFRSYLPVTSVSRTFPAINSIVSGT
                     NSNKWEVLPSVARTLNFRFTVRDNHPGAGNNKSDDMVLTINGTAGPFAVTAPNTAVSW
                     AANSAQTVTWSVNSTNVAPVSCANVKISISVDGGYTFSTLVASTANDGSEVVSIPNTP
                     TATARIKVEAVGNIFFDISNTNFTISAPAACGDPTGLTSSAITNTTATISWTAVSGAL
                     SYDVDYKAASSGTWINAATATANLSADLSGLTLGTTYDWRVRATCDAGSGNYVQAQFT
                     TTAPCNAPAGLSSSAITASSATVGWTAVSGANNYTVEYKTTAVSTWTTAASATTSTSQ
                     AISGLSASTTYDWRVRANCASGSSDYSQAQFTTSAAPVCPGTYDISTNGTRGGAATIP
                     FNTDIKGLVNPSGDNDYYKFVITTGGTITMTLTTLPANYHLRLLNSSGSTLQTSSNSG
                     TTNETINRTVTAGTYYARVYPSSSSQWNATNCYTLRVALGTASREEGLITGKLVSVFP
                     NPVNRTVTISIPEIQGMADIRVFDIYGKLMQQRSSGQMNTQLDLSSLSSGIYVIKVMN
                     NGKESMMKIVKQ"
     gene            complement(8215..9045)
                     /gene="kduI"
                     /locus_tag="PLO70_09630"
     CDS             complement(8215..9045)
                     /gene="kduI"
                     /locus_tag="PLO70_09630"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQZ74768.1"
                     /translation="MEIRFQNSPKETSEMSTQQLRDHFLVQGLMQPGKIELVYSHYDR
                     MIIGGVVPVSKVVSLPNEVELKAKFFLERREMGIINVGGKGTVTADGKVYDIEKLECV
                     YLGKGTQEVNFSSLSSEEPAMFYLLSLPAHHTYPNRKMTKAEAAPVNLGDISTSNKRT
                     IYKYIHNDGIESCQLVMGLTVLEDGSVWNSVPPHTHTRRMEAYFYFNLHEAHRVMHFM
                     GEPQETRHLVVANNEAAISAPWSMHFGVGTANYGFIWGMAGENKEFTDMDQVAIASIK
                     "
     gene            complement(9069..9830)
                     /locus_tag="PLO70_09635"
     CDS             complement(9069..9830)
                     /locus_tag="PLO70_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ74769.1"
                     /translation="MINFRVDNKIALVTGCSRGIGKTIAIELANAGADIIGVSNSLVA
                     KDSDIAKAIEATGKKFYPYAADFSDRDSLYNFLNKVKTDHPQIDILINNAGHILRKPV
                     ANHPDDYWDKIIAINLNAQFIITREIGKRMVERKAGKIVFTCSLLSFQGGINVPGYAA
                     SKGAIASLLKSFANEWAPHGITVNGVAPGYIDTDNTAQLQADPERSAAILSRIPAGRW
                     GKTSDLAGAFIFLSSPASDYINGSIITVDGGWMGR"
     gene            complement(9848..10744)
                     /locus_tag="PLO70_09640"
     CDS             complement(9848..10744)
                     /locus_tag="PLO70_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ74770.1"
                     /translation="MKKLLLICVVFIAFIASAFSQTADTVYKPVEYPAGFTAQLNVVY
                     TRVKDWEGKMDIYLPPKDKGPSPVVINIHGGGWNKGVKESQTGFSTFFKKGFAVANIE
                     YRLTGQATAPAAVEDTRCALIYLIKNAAALNIDVNRIVIMGGSAGGHLALMGGLLGND
                     YRFDGNCPGVQNIKVAAIIDKYGITDVWDWGYGKNLTSKSATRWLGDKANDKEFAMSV
                     SPVNYVTKNSPPIFIVHGNADPTVPYQQSVELYKKLQELGVQSEFITVEGGLHGKFEK
                     EKNSEINNAMIRFIFSLPAFKK"
     gene            complement(10763..12307)
                     /locus_tag="PLO70_09645"
     CDS             complement(10763..12307)
                     /locus_tag="PLO70_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ74771.1"
                     /translation="MKPIQYILLIGCLGLFSCKKVIDLYPQSNLNTGTYYSNLEEVKA
                     ALTGCYNGMQRSLFNEWQFTELRSDNAKQGAPGSTASANRDLSDLDMFIPSTSQQQIY
                     NYWLATYNNIRNINIVLQKLGVVYDPATGVLTFNSITIPITETDRKQLAGEAMFIRAY
                     HYFNLVRLFGGVFLVHEPVTPDQAKTMNRVSVADIYKLIEADLITAASSMSSLKFNQI
                     AAADIGRVTSWASKGLLAKVYLTLGRKTDAVPLLQDVIANSGYSLQSSYANVFSISNE
                     MNSEILFAVRYKAGGFGLGSHFGNTFAPLGTGSAVINGDGDGLNYPTAELDTLTNGDA
                     RKPTLIGVFGTGAAAKWYVKKFLFPVTLVDDGESDWPVLRFADILLMLAEAQGYSQAS
                     LDLINSVRPRAGLPNHTTATITSVAQFEQALSTERRIEFAFENQRWFDLVRFNTTMTT
                     VTAEQSIKDHYADEYYAHYRLYLPPTPTLAELQGNITRDKLLLPIPQREIDTNTQLVI
                     PQNPGY"
     gene            complement(12326..15526)
                     /locus_tag="PLO70_09650"
     CDS             complement(12326..15526)
                     /locus_tag="PLO70_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ74772.1"
                     /translation="MKKILCLSLTLLMLGSVAMAQKKTITGKVLNQETGEPLQGVSIL
                     VDKQKGGATTKADGTYSVSVDKTTTTIVFSYVGFASQTIAIADKTTVDVSLVPVVIDN
                     SEVVVIGYGTQKKSHLTGAVSKYKNEKLDETPSSRLDQALQGKIAGVQIQNISSEAGS
                     DPKVRVRGTSSINAGANPLVVVDGHPVPDGLAFVNMADVESVEVLKDAASSAIYGSRG
                     ASGVIIITTKSGKAEKPKYSVKFSTGSKTQYELYPMMSTTEYTNLLFYEASLKAKDPS
                     ITPPTGSGIIANNERAAYIVESTIMGGFGADWQQEALRDATVRNLQMNVSGGTRQLKY
                     FISGGYQRDQGMMYHSEYDRFNLRTRLDAQLSKKVKLTFNLNPSYIKRERPSVNFIDF
                     VRFQSFLPVYHTEASAAFVSQVPLWANIKPGDFAQARHFNGRVYGGLMPDGSIWTTSS
                     PTDPFNTANNTPKSAMETRTITSNDYRVLSSGDLSYNILPGLDFKTMGSIYMNYTQGL
                     DFAKRNSNREGDINRGVYTNRLFVDLLSENTLNYVRQIKDHSINVLAGFTAQKTKIND
                     EQVTGLDYPSDNVTTLNTALQIDQAKTATFNLKNQIGLLSYLGRVSYTYKNKYLLSAS
                     YRADGSSYFAPGNKWGSFPSISLGWVATEEKFTESLDWLSNLKLRASYGATGNNRIVD
                     FAFVDLLYAANYPLGSGTGTSVIGQVPSRDILSNPDITWERTFQYNGGIDLGLFKNAI
                     TLSMDVYQSKTDQLLLRQSAMGFAGVPLAWNNIGRLQNRGIEFEISTNNIRRKDFRWT
                     TSANIAHNKNKILELGEEAFLLNQGERTELYMNRVGDPLIQYFGYKTDGVWLSQADID
                     AARTAGLSSPLSNIFVPGGLKLVDVNGDNIINADDRVVTGSPYPDFTWGITNNITYKA
                     FDLSIMFQGVEGGQLINGDPNYNETKRYNKNYNNNRWLSPMFPGDGKTPYSTVGFNWM
                     LTDYVVEDASYFALRELIVGYTLPAKTAKSIGLSSMRVYFSGQNLYFQSAAGYRGINT
                     EARFTTGPYNTPLVDGYQRGSFPMPKTILFGIDVNF"
     gene            complement(15555..16760)
                     /locus_tag="PLO70_09655"
     CDS             complement(15555..16760)
                     /locus_tag="PLO70_09655"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HQZ74773.1"
                     /translation="MKKVLLFIVGLSIISCQATNAYKQPGKPVVYTLDLQTLDKNKGR
                     VNANDPVVMEAYKLLLKDADKALEFGPVSVMEKKHTPPSGNKHDYMSLAPYHWPDPAK
                     LDGLPYIRKDGQTNPEVKEYKDKEYMPKLCSDVLTLALAHYFSGEKVYAEHAVKLLRV
                     WFLDTATKMNPNLNFAQAVKGVNTGRGAGLIDSRHFVKVIDAIGLLQGSKYWTIADQQ
                     GMKQWFTDFLQWMQTSPIGLDEMDAENNHGVWYDAQRLAMALYIGNSELAKRIVLNAA
                     DRLDKQMDNDGKFPKEMARTISLHYSTFVLHAFFTIAQMAEKTGIDLWSMTTASGKSL
                     KKAFETLRPYLSQEKNWEGPQIKAFEYEDGYPILLEGALRFGCKHCKDDVMRLAGDKS
                     GKLRLTLLY"
     gene            complement(16768..>17749)
                     /locus_tag="PLO70_09660"
     CDS             complement(16768..>17749)
                     /locus_tag="PLO70_09660"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HQZ74774.1"
                     /translation="QLKFEGQANPVLTATYTGFVLGETATVLLTPAVLSTTAVLASPP
                     GTYPITVSGATSDNYDISFVNGVLTVQPKQNQIITFNALPTKTYGNADFAAGATSTNN
                     TIPVTYTSSNTAVATIIGSTIHIVGAGTSTITASQAGNVGYFPAADVARTLTVNKVTL
                     AVRVMDTTKVQGEVNPEFRVTYTGFVLGETTANLTTVPVVSTIATTNTSPGYYTLTVN
                     GGVSQNYNFTYTNGRLTIYPPGGNGQQYINAFMSNSSTLTVRVFSVEPALGDIILFDM
                     SGRQLAKKNLFMPAGFANADIPVSALPSGIYIVAVRGTGVELTKTIPIIK"
BASE COUNT         4977 a         3945 c         3485 g         5242 t
ORIGIN      
        1 ctgaatttta aaatcgccat accacaaaat taaggcttat tgggtattac cgtattagag
       61 atattaatta atataatagt attataaaat aatggcccga aggggaccgt tgtgaaaaag
      121 aggcaccctg aacgactttg ttttaccaca atgactcgca tattctcaac gattctttga
      181 tgctttttta ctttaagctg atatactact agttatctca aaaattaatt tttcattatt
      241 taaccacggc gggcacagcg attcacagcg ggaacaaagc cgtgtcactc tgttttcgct
      301 gtggttatga aagtcttgag aaagtttcta ttttttaacc tgcttcaatc ccagttcttt
      361 tgccttctcc agcataaaag catgtgcggc ttcataggtg ttggggataa cgccatccag
      421 catggcatct ttcaatgcat ctttgatgac acccacttgc cttgatggcg gcaggccaaa
      481 tatttccatg atctgttcac cactgacagg tggttgccag ttacgtacat ggtctttttc
      541 ttccacttct ttgcaacgaa ggcgcaccag ttgaaaattt tctaaaaacc gttttacttt
      601 ttgtttattc tttgatgtaa tatctgcatt gcaaagcagc atgagtgaat caaaatcatc
      661 gccggcatca aacaataaac ggcggatcgc tgaatcagta atattttctt ttgttaagct
      721 gatgggacga agatgcagtt ccaccatctt tcttacaaac cgcatttttt cattcaaagg
      781 cagtttcagt ttggcaaaga tctttggtac cattctgccg cccaccactt catgcccgtg
      841 aaaggtccag ccatgccctt cttcaaaacg tttggtggcc ggtttggcaa tatcatgcaa
      901 gatggcagcc catcgcagcc agagatcatc cgaattagcg gacaaattat ccaatacctg
      961 taaagtgtga tagaaattat ccttatgtcc aaggccgtct ttgtattcag ccccggccag
     1021 gtccaccatt tgcggaaaaa tgagctgcag caaaccactt ttatacaaca gatcaaagcc
     1081 aacagaaggc ctgggtgctg ccactatttt attcaactca tcagtaatac gttcctgcga
     1141 aattattttt atccgctctg cattatcaat aatcccctgc caggtacctt cttcaatgat
     1201 aaatcccaac tgggtagcaa accgaatggc cctcatcatc ctgagcgggt catcagtgaa
     1261 agtttgagca ggtgctaatg gggttcggat tattttatca tcaatatctt taattccatg
     1321 gaaaggatca atgagtgtcc cgtaattttc tttatttaaa ctgatggcta atgcattgat
     1381 ggtaaaatcg cggcggtcct ggtcatcttt caccgtgccg ggtaccactt ccggtttccg
     1441 ggaatcaaac cggtaacttt ctttccgggc gccaacaaat tcaatatcaa attcattttc
     1501 gagcctgaaa tgagccgtac cgaaattttt gaaataatca acatgcggct gtggccggaa
     1561 tttcttcgcc acttctttgg caaggtcaat ggcatcaccc acgcaaacaa aatccgcatc
     1621 tttggtttcc ctgcccaata ttttatcccg tacaaaacca ccgatcaaat aggcctccat
     1681 ccccagcgtt tcagctgctg tggcgacttt ttttaataat tccaattcct cttttgtaca
     1741 attgatatcc ataaagcggc aaagataacc tgaagaaggc agaagtctgg cgatacaggt
     1801 tcagggacgg agtacctgca ctttaccatt ttgccagcgt atcacagagg agggtgctgc
     1861 aacagttgta tcatcctgcc ggtagtttac gaggtaatca acaccttctt ttatctccgt
     1921 tgaaatatct gtaaaaagac gggccgccgg ctgaccggaa atattagcgg atgtggacac
     1981 aacaggtttc ctgaatcttt tcagcaaatg tttgcagaat ggttcatcac aaatacgaat
     2041 agcaatgctg ccatcttgac ccacaagatt ttcagccaac ccgatagcat cctcataaat
     2101 aacggtggtt ggcctggtgg ctccttccaa ataatcgaac acactcagat ccggactggc
     2161 cacatatttc aacacatccc tttcatcagc caccagtacg atcattgctt tttcatcaga
     2221 acgtttcttt aaccggtata ctctttccac tgctttttca ttggtagcgt cacagccgat
     2281 gccccagacc gtatcggtcg gataaagaat caggccacca tcatggagta ctttcaggct
     2341 ttgttcaata tctttttcaa aatttaccat atacttttat agacattcat aacagcatcc
     2401 gcacattttt gttgcgtaaa attttgtgca tgttcccagc ctttttcctt taatgtggcg
     2461 gctaatcctt tatctctgta aatttttttc ataccggttg ctatttcttc agcactggcc
     2521 gggttcacat aatatgctcc ggaaccgcct gcttccggta agcaggaaac actggatgta
     2581 ataacgggta accggctcca taaagcttcg agtaccggga taccaaaccc ttcgaaaaaa
     2641 gaaggataga tcattgcaat ggctgattga taaatagcag gaaatacttt agccgaacga
     2701 aaggacggcg aggatcttgc tttttcatgc tcagacaaaa agatcacttt tgaccccaat
     2761 ccattttgtg taacaaaatc tttgacctgt tgtttatact ttcccccctc accaataacc
     2821 agcagcggaa tatccagttc attccgtagt aaaaacaaag ctttacagat attcagcagg
     2881 ttttttcttt ctataacaga acctacatat agaaaaaatt gttccggtaa atcatactct
     2941 tcccttatcc tctttctttc ttcttcgttc acctcttcac caaaagcggg attacaactc
     3001 tggtagcaaa cagtgatctt attctccggt gttttataga attcaatgat gtctcttttt
     3061 gtctgttcac tgatggcaat tactttatcc gcatgttcgc aggcatagcg gaatttttta
     3121 ctgtaaatct ttacatcaat tgcattgtat tgctcagggt accgttcatg gatcaggtca
     3181 tgaatagtga caactgattt tattcctgtt cgttctatac cgaccggtat ctcatgacta
     3241 agaccatgat aaagaccgat cttcaatcgc tgcagatcct ttttcaccca actgcttctc
     3301 catgcggaac tgaacaacct gcttaaaaaa tctgttggaa caatctcctg taaattattt
     3361 cctttcagtt caaattcgga cgaatgcctg ggattaaaca ggtaatactg gtgctccggg
     3421 tgataatccg ataaggaccg tatcaacgta cggctataat gccccagacc ggtaccattg
     3481 tgaaaagccc tttttgcatc gaacccgata ttcatacgcc aatttgaaaa tgtgaaaata
     3541 acttaatttg aaaatgaaat cgtttatatt aaatagttgc cggaaacagc gtcattttca
     3601 cattgagcca ttcatttatc ttcgcagcaa attttacgaa atgaaagaat tgatactgga
     3661 agcatggggc aaccgtgact tgttaaatga taaaaaatac agtgatgccg tcagggccgt
     3721 gattgaagaa gttgataaag gcagactgag ggtggccttt cctgcggaca acggatgggt
     3781 ggtgaatgaa tgggtaaaac aggccatcct gctctacttc ggtatccagc aaatgcagac
     3841 ctgggatctg gctccatttg agttctatga taaaatgctg ttgaagaaaa actataaaga
     3901 acttggtgta cgggcggttc cgcatgcggt ggcccgttat ggtgcttacc tggctaaaaa
     3961 tgtggtattg atgccatcgt atgtcaatat cggtgcctat gtggacgaag gcacgatggt
     4021 ggatacatgg gctaccgttg gcagctgtgc acaaattggg aaagctgttc acctgagtgg
     4081 tggcgttggc attggcggtg tgctggagcc attgcaggca agtcctgtta ttattgaaga
     4141 tggatgtttt attggcagcc ggtgtatagt tgtagaaggt gttattgtgg aaaaagaagc
     4201 agtattggga gccaatgtgg tattaaccca atcaacaaag atcattgacg taagcggacc
     4261 ggaacctgta gaaatgaaag gaagggttcc ggccaggagt gtggtaatac ccggttctta
     4321 caccaaaaaa tttcccgccg gtgaatttgg cgtaggctgt gcccttatta ttggtcagcg
     4381 aaaaccaagt actgatctga agaccagttt gaatgatgcg ttaagggatt tcaacgtttc
     4441 agtttagaca cttatatttg cacccccgaa gcccgggtgg cgaaattggt agacgcactg
     4501 tcttcaggtg gcagcatccg aaaggttgtg ctggttcgaa tccagtcccg ggcacaaaaa
     4561 ccccgacaaa attgtcgggg ttttcttttt ctgttgttta accaatcttg aaaatttcaa
     4621 aacaaccggg gtcaggcata agggtttaaa cttatttttt ttcaaagatt gcagccgtgc
     4681 tttttaaacc tgccggttat ttattcacaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nntttttcaa agattgcagc cgtgcttttt aaacctgccg gttatttatt
     4861 cacaatcatc tttcccggat taaaatcatt tgtgcgttat agtaattaaa aattaaattg
     4921 ggttttaata aatatgtttt actaaaccca aaactctctt ttatgagaaa ccttttactt
     4981 agtgcaactt tactgttgtg tcattatttt gttacggcac aacaaaattt ttggcaaccg
     5041 gcagaagaat cagcgatcgg taaagattta tttactgaac gccaaaaacc tgccgcctac
     5101 aaaatatttc agttaaatga atctgctttt gcaacagcaa ccgggcttgc tccatcagag
     5161 aaatctatcc ctgccagcca atccacttct gttatcggca ttccgaatgc ggaaggccga
     5221 atagaaaact tcagggtggt agatgcccct gtgatggatc ctttattggc agccaggtat
     5281 cccggtatca gatcgtatgc cggtgtttca gttacggacc cttctgtaac aatacggttc
     5341 agtgtatctc cgcagggagt tagtgctatg atactttctg cttcgagacc taccatctat
     5401 attgatccgc tatcagataa ttattacctg gtagtttcaa gaactgatgt atccaactac
     5461 aggcagccat ttacctgtga aacaatgaat gcgatagctg aagtggccga taccccggcg
     5521 agtacactta gaaatgccga tgacggaagg ctgaggactt accggcttgc attggttgcc
     5581 agtggtgaat tttcacaata ttggctgaat ggtactgaaa ccactgatgc ggaacgtaag
     5641 gcaaaagtat tagcagctat gaatgataaa atgacaaggg tgaatggaat atatgaaaga
     5701 gatttcggtg ttcgcctgtt actgattgcc aataatgacg ccgttattta tctcaacgct
     5761 gccacagacc caattggcac aacatccagc agctggaata cacagacaca aaccacctgt
     5821 acaaatgtaa ttggcagtgc aaattatgat gttggacacc ttgttcaccg tggccctacc
     5881 aataatggca atgctggttg tatcggctgt gtttgtaaaa caaataaagg aagcggatgg
     5941 acttcttata ttgatctttc aagtgattat tttgttgttg actacctgac gcatgaatta
     6001 gggcaccagt ttggcggcaa ccacactttt acgcatgcaa acgaaggaac aattgcacag
     6061 gtagaaccgg gtagtggttc cactattatg agttatgcag gtattacagg cgccactact
     6121 gatgtgcaat cgcataatga tgattatttt catgccatta cgattcagca ggttactgat
     6181 tatataaaat cagccaacgg aacctgtgct gtttctactg ttacaggtaa tgcaactccc
     6241 acagctaatg ccggtgtgga ttatgttatc ccgaggtcaa ctccttttgt actttccggt
     6301 acaggcagtg acgcagatgc gggagatgta cttacttatt gctgggaaca aaataatcca
     6361 agggcaacag gatcttcaac ggtacccagt gcaactgcta cagcagggcc acaattcagg
     6421 tcttacctgc cagttacttc agttagccgc acttttcctg cgatcaattc catagtgagt
     6481 ggcacaaatt ctaacaaatg ggaagtatta ccttctgttg ccaggacatt gaatttcagg
     6541 tttacggtaa gggataatca cccgggagcc ggcaataaca aaagtgatga tatggtgctt
     6601 accattaatg gaactgccgg gccgtttgct gttacagctc ctaacactgc ggttagctgg
     6661 gcagctaatt cagcacaaac tgtgacatgg agtgtaaact ccaccaatgt ggccccagta
     6721 agttgtgcca atgtaaaaat ttcaatatct gttgatggtg gttatacctt ttcaacatta
     6781 gtggccagta ccgccaatga tggttcagaa gtggtgtcaa tccccaatac acccactgct
     6841 actgcaagaa taaaagtaga ggctgttgga aatattttct ttgatatctc caatactaat
     6901 ttcactatca gtgctcctgc agcttgtggt gatccaacag gactgacttc atcagccatc
     6961 accaatacaa ctgcaaccat tagctggaca gctgtaagcg gggcgctttc ttacgatgtg
     7021 gattataaag ctgcatcgtc aggcacctgg atcaatgctg ctacggccac tgctaacctg
     7081 tctgcggatc tatcagggtt aacattggga acaacctatg attggagagt gagagccacc
     7141 tgcgatgcag gaagtggtaa ttatgtacag gcacagttta ccacaactgc tccttgtaat
     7201 gctcctgccg ggttaagctc atcagccatc actgcttcat cagctacagt gggatggact
     7261 gcagtttccg gagccaataa ttatacagta gagtataaga caacagcagt atccacatgg
     7321 acaacagctg ccagtgccac tacttctact tcacaagcta ttagcggatt gtcagcatcc
     7381 acaacttatg actggagagt tagagcaaat tgtgcttcag gcagcagtga ctacagccag
     7441 gcgcagttta caacatccgc agcaccggtt tgtccgggta catatgatat ttcaaccaac
     7501 ggaacaagag gcggtgctgc cactattcca ttcaatactg acattaaagg attggtcaac
     7561 ccaagcggag ataatgatta ttacaaattt gtcattacta cgggcggtac aattacaatg
     7621 accttaacca ccctgcctgc caattatcat ttacggttat tgaacagcag tggcagtaca
     7681 ttgcaaacat ccagtaacag cggtactaca aatgaaacga ttaacaggac tgttactgca
     7741 ggtacctatt atgcacgggt ttacccaagt agtagtagcc aatggaatgc tacgaactgc
     7801 tatacactaa gagttgcatt gggtactgct tcccgtgaag agggattgat taccggcaaa
     7861 ttagttagtg tattccccaa cccggtaaac agaactgtca ctatttcaat accggaaata
     7921 cagggaatgg ctgatatcag ggtttttgat atttatggta agctgatgca acagagaagc
     7981 agcggacaaa tgaatacaca gctggatctg tcatcattat catcaggaat atatgtgatc
     8041 aaagtgatga ataatggtaa agaaagtatg atgaagattg taaaacaata agtattgttt
     8101 taaaaaaaat aaaaagcctt ccttttatcc gggaaggctt tttatttaaa tactatattt
     8161 tctgatcact cactttgaaa tgtctgaatg aatcattgag ctcaaggcac agatttattt
     8221 tattgatgca atggcaacct ggtccatatc tgtaaattct ttattttctc ctgccatacc
     8281 ccatataaaa ccataattag ctgttcctac tccaaagtgc atactccatg gcgctgaaat
     8341 agccgcttca ttgtttgcaa caaccaaatg cctggtttcc tgtggttccc ccataaaatg
     8401 cattacccgg tgtgcctcat gtaaattaaa atagaaataa gcttccattc tccgggtatg
     8461 tgtatgagga gggacagaat tccaaacact gccatcttct aaaacagtta atcccattac
     8521 gagctggcag ctttctatac catcattatg tatgtattta taaatggtcc ttttattgga
     8581 agtagaaatg tcacccaggt taaccggggc agcctccgct ttggtcattt ttctattcgg
     8641 ataagtatga tgggccggca gggataataa ataaaacatt gccggttctt ctgaagaaag
     8701 gctgctgaaa tttacttcct gtgtgccctt gcccaggtat acacattcca gcttttcaat
     8761 atcataaacc ttaccgtctg ctgtcacggt acctttacca ccaacattaa tgatccccat
     8821 ctcccttctt tccaaaaaga atttggcctt tagttctact tcattgggta aagaaacaac
     8881 tttcgatact ggtacaacac ctcctataat catacgatca taatgagaat ataccaattc
     8941 aattttacca ggttgcatca agccctgtac caaaaaatga tcccttaatt gctgggtcga
     9001 catttcactg gtttccttgg gactgttttg aaatcttatt tccattttta atttttaaaa
     9061 ctttattctt accggcccat ccaaccgcca tcgacggtta taatggaacc attgatataa
     9121 tctgaggctg gtgacgaaag aaaaataaaa gctcctgcca ggtcagaagt ctttccccag
     9181 cggccggcag ggatcctgga tagaatagca gcacttcgtt cagggtcagc ctgtagttgc
     9241 gccgtattat ccgtatcaat atatccaggg gctactccat taacggtaat accatgtggc
     9301 gcccactcat tcgcaaatga ttttaacaaa gaagctatgg ctcctttgct ggcggcatag
     9361 cccggtacat tgattccgcc ctggaaactc agtaaggaac aggtaaatac tatctttcct
     9421 gctttacgtt caaccattct cttaccaatt tccctggtaa taatgaattg cgcatttaaa
     9481 ttgatcgcaa tgatcttatc ccaatagtca tccgggtggt tggcaacagg tttacgcaaa
     9541 atatgtcctg cattattaat caatatatca atctgcggat gatcagtctt tactttattc
     9601 agaaaattat ataaggaatc ccggtcagaa aaatcagccg catacggata aaactttttc
     9661 ccggttgctt caattgcctt cgctatgtca ctatcttttg cgactaaact attgctaacg
     9721 cctataatat cggctccggc atttgccagt tcaatagcaa tggttttccc aattcccctg
     9781 ctgcaaccgg ttactaatgc gatcttattg tctacccgaa aatttatcat gctgcttata
     9841 ataaaaatta ttttttaaaa gcaggaagac tgaaaataaa ccggatcatg gcattattga
     9901 tctcactgtt cttttccttt tcaaatttcc catgcaatcc accttcaaca gtaatgaatt
     9961 ccgactgcac acccagctcc tgtaattttt tatacagctc cactgattgc tgataaggca
    10021 cagtaggatc agcgttgccg tgtacaatga atatgggcgg actattcttg gtaacataat
    10081 ttacaggaga aacagacatt gcaaattctt tatcattggc tttatcaccc aaccacctgg
    10141 tagctgattt actcgttaaa tttttcccat agccccaatc ccatacatca gtaattccat
    10201 atttatcaat gattgctgcc actttaatat tctgtacccc ggggcaattg ccatcaaaac
    10261 gataatcatt gcccaataag ccgcccatca atgcaaggtg tccgccggca ctgcctccca
    10321 taataacaat cctgttaaca tcaatattca gcgctgctgc atttttgatc aggtagatca
    10381 atgcacaacg ggtatcttcc actgcggctg gtgctgtagc ctgtccggta agacggtatt
    10441 caatattggc cactgcaaat ccttttttaa agaaagtact gaagccggtt tgcgattctt
    10501 tgaccccttt attccatccg cctccatgta tgttgatcac aaccggtgat ggccctttat
    10561 cttttggagg cagatatata tccattttac cctcccaatc ttttaccctt gtatagacca
    10621 catttaattg tgccgtgaaa ccagctggat attctactgg cttgtacact gtatctgctg
    10681 tctgcgaaaa agcagaagca ataaatgcga tgaatacaac acagatgagc aataattttt
    10741 tcatgtctta caatttttat tgtcagtatc cggggttttg cggaattact aattgtgtat
    10801 ttgtatcaat ttcccgctgg ggtatcggta ataataattt atccctggtg atattaccct
    10861 gcaactcggc cagtgtgggt gtaggtggca gatacaaccg gtaatgtgca taatactcat
    10921 ctgcataatg atccttaatg gattgttctg ccgtaacagt tgtcatggta gtgttgaaac
    10981 gaaccaggtc gaaccaacgc tggttctcaa atgcaaattc aattcttctc tccgtggaca
    11041 aagcctgttc aaactgggcg actgaagtaa tggtggctgt tgtatgattc ggtaatccgg
    11101 ctcttggacg aacactattg ataagatcaa gacttgcctg tgaataaccc tgtgcttcag
    11161 ccagcatcag caaaatatct gcaaagcgta agaccggcca atcactttct ccatcatcta
    11221 ccaatgtaac cgggaataag aatttcttga cataccattt tgcagccgca cctgtaccaa
    11281 ataccccgat cagtgttggt tttcttgcat ctccattggt aagggtatcc aattcagctg
    11341 taggataatt taatccatcc ccatcgccat tgatcacagc cgatccggtg cccaaaggcg
    11401 caaaagtatt tccaaaatgc gacccaagtc caaatccacc agctttatac cgaactgcaa
    11461 aaagaatttc tgagttcatc tcgttactga tggaaaaaac attggcatag ctgctttgca
    11521 aagagtatcc actatttgca atcacatctt gtaataaagg gacagcatca gtttttcttc
    11581 ccagtgttaa ataaaccttg gctaataacc cttttgatgc ccagctggta actctgccaa
    11641 tatcggcggc agcgatctgg ttaaatttca aactactcat ggaagatgcg gctgttatca
    11701 ggtctgcttc aattaattta taaatatctg ccactgatac acggttcata gttttggcct
    11761 gatccggggt aacaggttca tgtaccagga atacccctcc gaataaacga accaggttaa
    11821 aatagtgata ggccctgata aacatcgcct cacctgctaa ttgcttgcga tctgtttcag
    11881 taatagggat agtaatactg ttgaaagtta gtactcctgt tgccggatca tatacgactc
    11941 ccagtttttg caatacaata tttatattcc tgatattatt atatgtagcc agccagtaat
    12001 tatagatctg ctgctgagaa gtactgggta tgaacatatc caggtcactg agatcccggt
    12061 tagctgaagc ggtactgccg ggtgccccct gttttgcatt atcacttcgc agttctgtaa
    12121 attgccattc attaaaaagg gagcgctgca tcccgttgta gcaaccagtc agggcagctt
    12181 tcacttcttc caggtttgaa taataagttc ctgtattcag gttggattgc ggataaagat
    12241 cgatcacctt cttgcaggaa aataatccaa ggcaaccgat gagtaagata tattgtatag
    12301 gtttcatatt aaaagtttta atcggttaga agttaacgtc tatgccaaat aaaattgttt
    12361 taggcatcgg gaaacttccc cgctgatagc catccaccaa tggggtgtta taaggcccgg
    12421 ttgtaaatct tgcttccgta ttgatgcccc tgtaaccagc agcagattgg aaatataaat
    12481 tctgaccgga gaaatagacc cgcatcgaac tcaggcctat tgacttggca gtttttgcag
    12541 gcaatgtata accaacaatc aattccctca gtgcaaaata agaggcatct tcaactacat
    12601 aatctgtcag catccagttg aatccaacag tagaataagg cgttttgcca tcaccgggga
    12661 acatcggact gagccatctg ttgttattat agttcttatt atacctttta gtttcattat
    12721 agttgggatc accattgatc aattgtccgc cttcaacacc ctgaaacata atgctgaggt
    12781 caaaagcttt gtaagtaata ttattggtaa taccccatgt aaaatccgga tatggtgaac
    12841 ccgtaaccac cctgtcgtct gcgttaataa tattatcacc attaacatct actaatttca
    12901 atcctccggg aacgaaaata ttactcaacg gacttgacaa accggcagtc ctagccgcat
    12961 caatatcagc ctgtgaaagc cagacaccat ctgttttata accaaagtac tggatcagcg
    13021 gatcacccac acggttcata taaagttctg ttcgttcacc ctggttcaac aaaaatgcct
    13081 cctcacccag ttctaatatt ttattcttgt tgtgggctat attggcagaa gtcgtccatc
    13141 ggaagtcttt cctcctgata ttattggtag aaatctcaaa ttcaattccc ctattttgca
    13201 ggcgtcctat attattccag gcaaggggca ctcctgcaaa tcccatagca gattgcctca
    13261 ataacaactg gtctgtttta gattgataaa catccatgga aagagtgatc gcatttttaa
    13321 atagccccag gtcaatacct ccattgtact ggaaggttct ttcccatgtt atatcaggat
    13381 tggaaagaat atctcttgac ggaacctgcc ctataacact ggtaccagtg ccagaaccta
    13441 acgggtaatt ggcagcatat aaaagatcaa caaacgcaaa atcgacaatt cggttattac
    13501 cggtagctcc ataactggct ctaagtttca aattgcttag ccagtcaaga ctctctgtga
    13561 atttttcttc agtagccacc catcccagcg atatagaagg gaaagagccc catttatttc
    13621 caggagcgaa ataagaactt ccatctgccc ggtaactggc ggataagaga tacttgtttt
    13681 tataagtgta actcacacgg ccgaggtaag aaagtaatcc gatctggttt ttcaaattaa
    13741 aagtagcagt ctttgcctga tctatctgca atgcagtgtt tagtgttgtt acattatcac
    13801 ttggataatc aaggccggtt acctgttcat cgttgatctt ggtcttctgt gcagtgaaac
    13861 ctgccaggac gttgattgaa tgatctttaa tttgcctgac atagttcaac gtattctcag
    13921 acaacaaatc cacaaaaaga cgattggtat aaactcccct gttaatatct ccttccctgt
    13981 ttgaatttct cttggcaaaa tcgaggcctt gtgtataatt catataaata cttcccatag
    14041 tcttgaaatc aagacccggc aatatattat acgaaagatc gcctgagctt aacacccggt
    14101 agtcattgga agtgatcgtt cttgtttcca ttgcagattt gggtgtattg ttcgctgtgt
    14161 taaaaggatc tgtaggtgac gatgttgtcc atatacttcc atcaggcata agcccgccat
    14221 aaaccctccc attaaaatgt cgtgcctggg caaaatcacc gggttttatg ttagcccaca
    14281 atggcacctg gcttacaaaa gcagcgcttg cttcagtatg ataaacagga agaaaagact
    14341 ggaaacgtac aaagtcaata aagttcactg atggcctttc ccttttgatg taagatgggt
    14401 tcaggttaaa ggtcagtttt accttcttgc tgagctgcgc atcaagcctg gtccgcaggt
    14461 tgaaacgatc gtactcacta tgatacatca taccctggtc tctctggtaa ccaccggaaa
    14521 taaagtattt caactgcctg gtaccacctg aaacattcat ttgcaggttt cttactgtag
    14581 catcacgaag agcttcctgt tgccagtctg ccccgaagcc acccatgatt gtactttcaa
    14641 caatgtaggc agctcgttca ttattggcaa taataccaga accagttgga ggagtgatac
    14701 tcggatcttt tgcttttaaa gaagcttcat aaaaaagcag gttggtatat tcagttgtac
    14761 tcatcattgg atacaactca tactgtgttt ttgaacctgt agaaaatttt acagaatact
    14821 ttggcttctc cgctttaccg cttttagttg tgatgataat aacaccactg gctcctcttg
    14881 atccataaat agcagaagag gcagcatctt tcaatacttc aactgattcc acatctgcca
    14941 tattcacaaa tgccaaacca tcgggaacgg gatggccatc tacaactacc agcggatttg
    15001 caccggcatt aatagagctt gtacctctta ctctcacttt aggatctgaa cctgcttcag
    15061 acgatatatt ctgtatctga acaccagcta tttttccctg cagtgcctgg tcaagtcttg
    15121 aagatggcgt ttcatccaat ttttcattct tatacttgga tacagctccg gtaagatgag
    15181 atttcttctg ggtaccatac ccgataacca ctacctcgct attgtcaatc acaaccggaa
    15241 ccagcgatac atccactgtt gttttatctg ctattgctat tgtttgtgat gcaaacccaa
    15301 cataagaaaa gacaattgtg gtagtagtct tgtcaacaga cacggaatag gtaccgtctg
    15361 ctttagtagt ggccccaccc ttttgtttat caacaagaat actgacccct tgcaaaggtt
    15421 cacctgtttc ctggttaagt acttttccgg tgatcgtttt cttttgcgcc atagcaacac
    15481 tgcccagcat gagcaatgta agagacaagc acagaatttt tttcataata gcagtttaaa
    15541 tatttacaaa tcgtttagta taataaagtg agacgaagct ttcctgattt atctcctgca
    15601 aggcgcatga catcatcttt gcaatgttta catccaaagc gaagagctcc ttccagtaaa
    15661 attggataac catcctcata ctcaaaagct ttgatctgtg gaccttccca gtttttctcc
    15721 tgggagagat aaggacggag tgtttcaaaa gctttcttta aagattttcc tgatgccgtg
    15781 gtcattgacc aaaggtcaat accggttttt tccgccatct gtgcaatggt aaaaaatgca
    15841 tgcagtacaa atgttgaata atgcaacgaa atggttcttg ccatttcctt agggaattta
    15901 ccatcattgt ccatttgttt atccaggcgg tcagcagcat tcaatacaat tcttttggcc
    15961 agctcactat taccaatgta taaggccatc gctaaccgtt gtgcatcata ccaaacccca
    16021 tgattatttt cagcatccat ttcatccagt ccgatcgggc tggtttgcat ccattgcagg
    16081 aaatcagtaa accattgttt cataccttgc tgatctgcta tggtccagta ttttgatcct
    16141 tgtaataaac cgatagcatc tattactttc acaaaatgac gggaatcgat caatcctgct
    16201 cctcttcctg tattcacccc tttaacggcc tgtgcaaaat tcaggttagg gttcatcttt
    16261 gtggctgtat ctaaaaacca aactctcagc aattttactg catgttcagc atagactttt
    16321 tccccggaga aataatgagc caatgctaac gtaagtacat cactacaaag tttcggcata
    16381 tactctttgt ctttgtactc ttttacttcc ggattagttt gcccatcctt cctgatataa
    16441 ggaaggccat caagctttgc aggatcaggc caatgataag gtgccaggct catataatca
    16501 tgcttgtttc cactgggggg tgtatgcttc ttttccatca cactcaccgg cccaaattcc
    16561 aaagctttat ccgcatcttt cagtaataat ttatacgcct ccatcaccac aggatcgttg
    16621 gcattcaccc tgcctttatt tttatccaaa gtctgcaaat ccaacgtata cacaactggt
    16681 tttccgggct gcttatacgc attagttgcc tggcaactga tgatcgataa tccaacgata
    16741 aataacaaca cttttttcat agttatttta tttaatgatt ggaattgtct ttgtcagttc
    16801 aacacctgta ccccttactg caactatata aataccagat ggcaatgcag ataccgggat
    16861 atcagcgttg gcaaatccag caggcatgaa gaggttcttt ttggccaatt gtcttccgga
    16921 catatcaaat aatataatat cacccaatgc tggttctaca gaaaataccc gaaccgttaa
    16981 tgtactgctg ttgctcataa atgcattaat atactgctgg ccgtttccac cgggaggata
    17041 aatggtcagc cggccattgg tataagtaaa attgtaattc tgggaaacac ctccgttaac
    17101 tgttaaagta taataccccg gagaagtatt cgtagtggca attgttgata ccaccggcac
    17161 tgttgtgaga tttgcggttg tttctccaag gacaaatccg gtataagtaa ccctaaattc
    17221 cggatttact tcgccctgta cttttgtagt atccattact cttacagcca gcgtaacttt
    17281 atttacggtc aatgttcttg ctacatcagc agccgggaaa taacctacat tacctgcctg
    17341 tgatgcagta atggtagaag taccagctcc aacgatatga atagtacttc ctataatagt
    17401 tgccacagcg gtattactgc tggtataagt tacaggaata gtattatttg tactggtggc
    17461 acccgcagca aaatccgcat taccataagt cttggttggc agcgcattaa aagtgatgat
    17521 ttgattttgc ttaggctgta ctgttaacac tccgtttaca aaactgatat cataattgtc
    17581 cgatgtagca ccgcttacag taattgggta tgttccggga ggcgatgcca gtacagcagt
    17641 agtactaagt actgcaggtg taagtaaaac tgttgctgtt tcacctaaca caaatcctgt
    17701 ataggtagct gttaatactg gattagcttg tccttcaaat ttcaattga
//
LOCUS       DAORTI010000067        17588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2887_length_17588_cov_6.550505, whole genome shotgun sequence.
ACCESSION   DAORTI010000067 DAORTI010000000
VERSION     DAORTI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17588
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_17588_cov_6.550505"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLO70_09665"
     CDS             complement(<1..822)
                     /locus_tag="PLO70_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ74775.1"
                     /translation="MKITIRGVRGSIPTSGPATQKYGGNTSCIEVSAEGWQLVLDGGS
                     GMRNSNTQERPLNNRLDILLTHLHMDHIQGLGFFKPFFDPEMEVHIWGPAGSRKSLHA
                     RLSKYLSPPLFPMYIRELPCQLILHEVDRSEFEIGPFRVQSQYVIHPGPTVGFRINDG
                     EKTFTYIPDHEPALGIHGIVSDQKWISGFDLASNADLLIHDAQYTTAEYKERTGWGHS
                     SMDDALRFASMACVKKLLMAHHDPNHTDTYLGELLTELKMRNNYSFHYDLAEEGMV"
     gene            complement(819..1214)
                     /locus_tag="PLO70_09670"
     CDS             complement(819..1214)
                     /locus_tag="PLO70_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQZ74776.1"
                     /translation="MAAYEIEIILNRQLADCLSIPVFITDREGNLIYYNEPAEQILGK
                     RYDDTGEMPVSIWATIFKPVDEKGNVLSPDDLPLVKTLKNCKPYHKTFCIESLNGKIE
                     KIAVTSYPILGRPSRFLGAVALFWETKDE"
     gene            1605..>2926
                     /locus_tag="PLO70_09675"
     CDS             1605..>2926
                     /locus_tag="PLO70_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ74777.1"
                     /translation="MNKSHNLLLLLLLIHSGLIAQVLVKNTNVLDVENKKILSGYDVV
                     VLEGRIISVEKGKQYKLPPGTDVIDGTGKYLVPGFADAHVHFFQSGGLYARPDVIDLR
                     KYQPHNTELKWVHDNMEDLLHRYTATGITSVVDVGASFNFLQQRDSLMTRTALPVVSM
                     TGPLLTTWLPPVYKDLGNEGPFTLMKTKEDAIIAVKEQLKYNADFIKIWYIVLDSNIE
                     AGARKNYEIVKAAIEEAHKNNKRVAVHAMERITAQLAVEAGADFLVHSVTDEYVSNEF
                     VQLLKKRNVVLCPTLVVGGNYSKVLSDNYQFSTYELEHAHPFTISTIIDYPWPDTLVG
                     KNYIKTLQYRKQTGKPTLEDTIMAVNLKKLADAGVTIATGTDAGNIGTQHATSYFDEL
                     SAMQQAGLNMWQLLQASTINGAKAVGQEKEWGSISKNKLGNMVLLNANP"
     assembly_gap    2927..3026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3441..5582
                     /locus_tag="PLO70_09680"
     CDS             3441..5582
                     /locus_tag="PLO70_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74778.1"
                     /translation="MRNPVVCSLILLLALLPVSIFAQTVTLVTANERLKEEQVDFSQY
                     IQSIFIDSNVAGRLQWFVEFEVDSINQSVAGDTGLTEVEKAKAIQSLVYFLKQLSENL
                     SLQKFDIYDIPDALESYKLTLRALLRHKPYLELVNKLGPRRSQLLANAFWQYNECTEL
                     EDIAVFKRVASLPAYILQFLESRPGFRYADSLLVLVAAHYPSKVVSYLLQHKPGLQLL
                     IRMNKNIYLQQIVSLSENRFISELLPFIIPLAEKRITVTEILDKRKDPKTYFELLVNT
                     LKHELSRPAIASSIFLIPLRNGIKDKSISFYVNQINELHSSAAEIRFAAVNDLRVEDL
                     YYIITSCEEELYTSSYLGLYRRLMEHFNTPAVDSLFGIVKYDNFRPFMRMAAIYNTLG
                     DFLSCMPQKTAAGLLKRFISGIETDANTGLAKAMDIADAFSGFTALPGISLMIQQELK
                     SNYDRCKYDQSFFGMRLYSILQNVFDLVQKKDSVNKFWANLGNHELLERKVLQNKRDE
                     IIEVVLFYGDEDGIGSFNNFIRLYKDPTRWKISKNNYWITIRSLSEQPVIIYANLPLD
                     NETEMDMLAQDSLFAFLKQQMAEPVILIHRGHSYHLSHTLRRLQPTVKLAILGSCGGY
                     NSLLNIANISPDAQIIVSKKIGSQFINDPMIDVINENLQNNRDLVWSEIWEKLRIRFS
                     KNELMLNLFNEYIPPTKNVSLFVLKLFNFYR"
     gene            complement(5628..7256)
                     /locus_tag="PLO70_09685"
     CDS             complement(5628..7256)
                     /locus_tag="PLO70_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQZ74779.1"
                     /translation="MKRYFIFICSLATLPIFAQDVKISKSIKQAMSRIDTNNIRSHIA
                     YLAHDRLKGRLPGTAGYQLAVDYVIDQYRQIGVTPGGDNGGFTQKVILRKSIVNNSSS
                     IAVLKDSEGNIDSLIFQNDFTPVPHPFSVMASAEGQLIFSGYGIDIPGGYSDYTGMDV
                     KGKIVVIINGAPDGLSSTFTAHFSSPGNKMNTAYARGAAGVIIINLNARPGSNPNPVL
                     QFNVALNADKSAAFGRVFTGNLKIALISNKKILTRLFLNSGKNLEQVLAGIKKGNPSS
                     FELPYSISASYKTTHNDFESYNVVGLIPGSDKVLKNEYVVHSAHLDHVGIGRVVNGDS
                     IYNGAHDNASGVASLLEIARIYKSGVKPKRSILIAMVTGEEMGLIGSSYFAANPTVPK
                     GSIVANVNTDMPTVIAPLLSIVPLGAEHSSIMNNVKFAASYLGLDIEKDPEPNENRFV
                     RSDQYSFVMNGIPALHIKYGNKSDTPGFDMDAYVKKWRAKYYHQSADGMDGIFNFAAA
                     KTYVQLNFLVSFSIAQTLLRPEWNKGDLFGTIAK"
     gene            7476..8303
                     /locus_tag="PLO70_09690"
     CDS             7476..8303
                     /locus_tag="PLO70_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQZ74780.1"
                     /translation="MQTKFYIPHLLLQEFVQCIMIVHAELDKNAAPVICPYPPTPQNS
                     LFFYINDPIKVQIEGKKDFLLQPKSVVVGQQLTRVMLDINKSHKAVRVGFHPGGLHRL
                     MGLSMADMIDGNYDAADIFGNEIQEVNNQLQEAEDFDAIRSIVERFLLQKVKKLKQAL
                     PFDKAMLELLRLNGNVSIEKIASLACLSLRQFERVSKERIGLPPKLFARLIRFSKAYR
                     MRENFPLLSWTQIAYECGYFDQMHFIRDFKQFAGVAPGILEKELENTPVRLQADLRL"
     gene            8367..9269
                     /locus_tag="PLO70_09695"
     CDS             8367..9269
                     /locus_tag="PLO70_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74781.1"
                     /translation="MQQYSFLFYLAFTLFMLHPAKAQQFDKLNELQDHSVKVYYSAGH
                     EQRASSIANRVDKAFNYQQQQLGFKPSVILLVLSISDWSNYTSFPVYGMPHYNDDKIL
                     IVAAEDNPFWKSFLPPLEQLPPALSEQIQTVYRRSDGSISMEAFFDLLALHELGHAFH
                     FQGGLTMQRKWMGELFANILLHTYVAENEPEQLPALTLFPRMVISGGTKEFTYTSLKA
                     IEERYDEIGQRHPKNYGWYQSRWHAAAGNIYDASGKSICSKLWEALKNNKEVLTDEEL
                     LRFLGKIHTSISDMMKNWDSNTVR"
     gene            9489..10976
                     /locus_tag="PLO70_09700"
     CDS             9489..10976
                     /locus_tag="PLO70_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74782.1"
                     /translation="MSVCFYRYTVPVSYTTGKMYPRSLFFSRKILKLFLFALFISTYT
                     FSQGSPEYGSGIKLNLSPEGNRYIRFISWNQVWFRSQQNNPGSLINWEVKNNTWDIGA
                     RRVRMIAYAQLSPRYLILAHWGINNQTFAGGGGFGSSGTGPSGAGKKPQLFFHDIWNE
                     YAIVPAKNPKTNTSNKNTLYLGAGLHYWLGISRMTSASTLNFMTIDAPIFNWPMIEVS
                     DQFMRQFGFYVKGKLGKLNYSASVNKPFATNSTPFYDSLKGRVAIDNNGDAKPSVQGY
                     ADYQFLDQEANVLPFRVGTYVGTKKVFNIGAGFYHNTDATKSTDLGGTIKKHNINLLG
                     IDLFADLPVGNKAKNMAITAYSVFYNYNFGPNYWRTLGVMNTSTGFDPMLPVANRAIN
                     GPGNARMFIGTGNIFYTQAGLLLPKGKSNKLRVQPFGAYTWKKFDYLGEPGNYFDVGA
                     NFFIDGHNAKITPQYSTRPLYYLRNGQHIKDGTKGEFLIQFQVYL"
     gene            complement(11013..12392)
                     /locus_tag="PLO70_09705"
     CDS             complement(11013..12392)
                     /locus_tag="PLO70_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQZ74783.1"
                     /translation="MSFFSREKTIAPPGYNRWKVPIAALSIHLCIGQIYAFSVFNQPL
                     TKVIGITESVPGDWKLTTLGWIFSIALFFLGASAATFGKWMERVGPRMAMLASALCFA
                     CGFMISSLGIHLHQIWLLYLGSGVIGGIGLGLGYISPVSTLIKWFPDRPGMATGMAIM
                     GFGGGGMIASPLSVYLMKQFSNETSVGVAQTFLVMGCLYFIIMMFGAVIVRVPAEDWK
                     PKGFDPAKAMAKKLVTNAHVLVDNAVKTPQFYLLFMVLMLNVTAGIGVLGQASVMIQE
                     LFSVESVGASKAVDASAAAGFVMLLSLFNMAGRFIWSSLSDIIGRKNTYTIFFSLGII
                     LYSSIPSIGASGSVVMFVATFAIIISMYGGGFATVPAYLRDLFGTKQVGAIHGRLLLA
                     WSMAAILGPVLINYMRQYQIEVSKLPKAQVYSTTMYVMAALLVIGLICNLSIKQVHEK
                     HHLNENSTK"
     gene            complement(12680..13630)
                     /locus_tag="PLO70_09710"
     CDS             complement(12680..13630)
                     /locus_tag="PLO70_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74784.1"
                     /translation="MYPKLLISQAVMLLLTVFLAGLTYTQELTIYSIPSPKGINWDSP
                     RKLVFSYINNTFARSRYGKEKHPIGHMIVEMKDSSRYTLVGTTSQAHSGMTKKVIRKG
                     YGLGILFEIINGRLDEEYVNLPQLKQRCETGDITFIKYRINQTVFNRLWQYLQEYKLK
                     GYDRLYNGFNKPREGLGAGCSAFAHSFLEVGGLEEVLPSNEWAVKVQVPEKLIGGNSE
                     KDKHVQFFKLLLSKQWAHPEKQPCRQLILYEPTILHNWILKKYNSISEGDAILKSKLG
                     KANGLIIDCTDKMPPEEPIWINNNKTEQNRMETDVRKKIL"
     gene            complement(13760..14212)
                     /locus_tag="PLO70_09715"
     CDS             complement(13760..14212)
                     /locus_tag="PLO70_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ74785.1"
                     /translation="MKRSFLLLGVVALITTTLLFMIVQQEDPWKVPEKYEKLKNPVVA
                     DESSIASGKELYDYFCHSCHGTDGKGSGKRALKFDNTPTDFTTAAFQQQTDGALLYKI
                     YSGHKEMPGFKKRIPGNQDAMEGSFGKTRIPGDIVNYVRTFGKKHSQH"
     gene            complement(14424..14969)
                     /locus_tag="PLO70_09720"
     CDS             complement(14424..14969)
                     /locus_tag="PLO70_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HQZ74786.1"
                     /translation="MNYPDKATIIQQTKKWIIDVVIGCNFCPFAAKEIKRDTIQYEVL
                     TDATTTTALQAVMTTMYQLDAAKHIETSLLIFPGSFELFEEYLHLLELAEKLLVKEKY
                     EGIYQIASFHPKYLFAGSNENDPSNYTNRSPYPMLHFLREESLSKAIDSYPDIDEVPN
                     RNIAFTREKGLRYMQQLLGKP"
     gene            complement(14979..15188)
                     /locus_tag="PLO70_09725"
     CDS             complement(14979..15188)
                     /locus_tag="PLO70_09725"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HQZ74787.1"
                     /translation="MHSNDPLHGKTLEMVLKELVHHFGWEKMGTIIKINCFTSNPSIN
                     SSLKFLRKTPWARKKVEELYIRYFT"
     gene            15450..16307
                     /locus_tag="PLO70_09730"
     CDS             15450..16307
                     /locus_tag="PLO70_09730"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQZ74788.1"
                     /translation="MQSLREKNIAILCNALAGSGSAVLCAENICTRLSDKNIRHTQFK
                     DTWPDDFSLYTDIFIVGGDGTLNYFINRYPGMSLPLVIFKGGTGNDVYWLLYGNSTLD
                     EQLETALNSSPKPLDIGKCNDRYFINGVGIGFEGEVAKALTGKKKLPGKTSFLITVLK
                     KIVTYRSKRYTIQSAERSLSGRRLLVDISNGRRAGGGFHIAPEARADDGLLDVIIADA
                     LTPLQRVRYLPVIEKGKHLHLSFIHHFRTRKISIEGDDLLQYHLDGEYFVAKRLNIEI
                     IPAAVTFRF"
     gene            16400..16876
                     /locus_tag="PLO70_09735"
     CDS             16400..16876
                     /locus_tag="PLO70_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74789.1"
                     /translation="MKAATINELKQELLNASSARLTELCLRLARFKKENKELLTYLLF
                     EAHDTDAYITSVKEEMALQFDAINRSNVYFVKKTLRKILRIANKFIRYSASAIVETEL
                     LIFFCLKLKAMGDSINKNPVILNIFQNQLKKINKSISAMHEDLQYDYQREVRALTV"
     gene            complement(16914..>17588)
                     /locus_tag="PLO70_09740"
     CDS             complement(16914..>17588)
                     /locus_tag="PLO70_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ74790.1"
                     /translation="GDHWQLPPTVLSNEAANMGFNESILERAIIKTNNVFLLNTQYRM
                     RQAIAGFSSNYFYNSLLLTAEHLYNTGVHLTFIDTAGSGYNEEHGPDGTSLQNEGELR
                     IVQKIIETESLNPLTTAFISPYSGQVAAAKEMLPKQLRLSTIDSFQGQEKEIIIVSLV
                     RSNDDGDIGFLKDYRRMNVAITRAKEQLFVIGDSATIGADVFYNSFLTYIEKHGTYRT
                     VWEFEM"
BASE COUNT         5233 a         3348 c         3531 g         5376 t
ORIGIN      
        1 caccatacct tcttctgcca ggtcataatg aaaagaataa ttatttctca ttttaagttc
       61 agtcaatagc tccccgagat acgtatcggt atgattaggg tcatgatgag ccatcaacaa
      121 ttttttaaca caagccatcg aagcaaatct cagcgcatca tccatagaag aatggcccca
      181 gcctgttctt tctttatact cggccgtggt gtattgggca tcgtgtatta acaggtcagc
      241 gttggacgca aggtcaaaac cagatatcca tttttgatct gacacaatac catgaatgcc
      301 caatgccggt tcatgatctg gtatataagt aaatgtcttt tccccatcat tgatccggaa
      361 gcccactgtt ggcccgggat gaatgacata ttgtgattga acccggaatg ggcctatctc
      421 aaattcactt ctatcaactt catgtaatat aagctggcag ggaagttcac ggatatacat
      481 cggaaacaaa ggcggtgaca ggtacttgct cagcctcgca tgcagtgatt ttctgctacc
      541 ggccggaccc cagatatgta cttccatttc cggatcaaaa aatggtttaa aaaaaccaag
      601 gccctgtata tggtccatgt gtaaatgagt caacaaaata tccaggcggt tattcagtgg
      661 acgttcctgg gtattggaat tgcgcatacc ggaacctcca tccagaacca gttgccatcc
      721 ttctgccgat acttcgatac aggatgtatt gcctccatac ttttgtgtag cagggcctga
      781 agtgggtatg gaaccacgga caccccgtat tgtaattttc attcgtcctt tgtctcccag
      841 aataatgcta cagcaccaag aaacctggat ggcctgccaa gaatggggta tgaagtgaca
      901 gcaatttttt caattttccc attcaggctt tcaatgcaga atgttttatg gtagggctta
      961 cagtttttga gcgtttttac caatggcaga tcatccggag ataaaacatt ccctttctca
     1021 tccaccggct taaaaatggt agcccatatg gacactggca tttcaccggt atcatcatac
     1081 cgtttaccaa gtatctgctc ggcaggttca ttatagtaaa ttagattgcc ttctctatca
     1141 gttataaaga ccggtatgga aagacagtcg gccagttgcc ggttcagtat tatttctatt
     1201 tcataagctg ccatataaca agaatagtaa ggtaattagc tacttaaata tataaaataa
     1261 atgagactta aactaacttt aaaataggac aggtttgaag tgggagatgg ctcagtaaaa
     1321 cattgttgta ataaatagaa gtagattgtt taaaaaagaa gagttattca gattgagtta
     1381 atcagcttca ttaccaccgt cctgttacta ttgttaaaca gtgaaaattt ccattactaa
     1441 acttgtagct taatttttta aatgatgtta taaaaaataa cgctattaaa taaaaaaaga
     1501 tagcgacttc tataaaaaaa ggaaacgata gctcttttag atcttactta ttatccagcg
     1561 taaactgttt ccacaatttt caatatatta caaaaaaaat gtttatgaat aagagccaca
     1621 atttgctgct ccttttgttg ttgattcact caggcttaat tgctcaggta ctggtaaaaa
     1681 atacaaatgt gctggatgtg gagaataaaa agatactgag tggatatgat gtggtggtat
     1741 tggagggtcg tatcatcagt gtggagaaag gcaaacaata caaattgcca cctggtacag
     1801 atgtgattga tggaactggc aaataccttg ttcctggctt tgcggatgca catgtgcatt
     1861 tttttcaaag tggtggttta tatgcaagac cagatgtaat tgacctgcgt aaataccagc
     1921 cccacaatac ggagttaaaa tgggttcatg ataatatgga agacttattg caccggtata
     1981 cagctacggg tattacatcg gtagtagatg taggagccag ttttaatttt ttacagcagc
     2041 gggattcatt gatgaccagg actgctttac cggtggtgag catgacaggg ccgttattaa
     2101 ctacctggtt gccgcctgtt tataaagatc tcggtaatga gggtcctttt actttaatga
     2161 aaacaaaaga agatgccatc attgccgtaa aggagcaact gaaatacaat gctgatttta
     2221 ttaaaatatg gtatatcgtg ctggacagta atattgaggc aggtgcacgg aaaaattatg
     2281 aaattgtaaa agcagcgatt gaggaagcac ataaaaacaa taaaagggta gcggtacatg
     2341 caatggaacg tatcaccgct caactggcag tagaagccgg tgcagatttt ttagtacaca
     2401 gtgtgacgga tgaatatgtc agcaatgagt ttgttcaatt actcaaaaaa aggaatgtag
     2461 tgctttgtcc cactctggta gtagggggga actattcaaa agtgctttcg gataattatc
     2521 agttcagtac ctatgaactg gaacatgctc atccgtttac tatcagtacc atcattgatt
     2581 atccctggcc ggatacactg gttggtaaaa attatattaa aactttacaa taccggaaac
     2641 agacaggtaa gcccaccctg gaggatacta tcatggctgt aaacctgaaa aaattggcgg
     2701 atgccggtgt taccattgct accggaacag atgcgggtaa tatcggtaca cagcatgcta
     2761 catcttattt tgatgaactt tcggcaatgc agcaggcagg actgaacatg tggcaattgt
     2821 tacaggcttc caccatcaac ggggctaaag cggtagggca ggaaaaggag tggggtagta
     2881 ttagcaaaaa taaattaggg aatatggtct tgctcaatgc taatccnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnaaca cggttattga ccatgaggag atatggttat
     3061 cacccaaacc ggataatctg cgttatggtg tggccatcta tgtgatacta aatggaaaga
     3121 tcagtaaagt gtattttgct gattgatata caaaagcatt cttgctgttt tcattctaag
     3181 atgcaattct tttatttcag aaataacaat tcagaacgga gaaatgacag ttgccttaat
     3241 gattttagtc attatttgcc tcttttacat ttagtacttt taatttaatt cattaacgat
     3301 tcagattgga gatttgtgta aaaatgtaac gtattttatt ataagtgtac aaacctgctg
     3361 ttgttaaagg gtttactcag gtatataatt aatagctttt cttttttaag tttaaatatt
     3421 atatcctata ttttatcgtg atgcgtaatc ctgttgtatg ttctcttatt ttgctattgg
     3481 ctctattacc tgtttccata ttcgcccaaa ctgttaccct tgtcactgca aatgaacgtt
     3541 tgaaagaaga gcaggttgat ttttcacagt atattcaatc tatttttatt gattccaatg
     3601 ttgctggaag gctgcaatgg tttgtggagt ttgaagtgga tagtattaat cagtctgttg
     3661 caggcgatac tggattgacc gaagttgaaa aggcaaaggc aattcagagt ctcgtttact
     3721 tcctgaaaca acttagtgag aatctctccc ttcaaaaatt tgatatatat gatattccgg
     3781 atgctctgga atcctataaa ttaacgttaa gagccctgct aaggcataaa ccttatcttg
     3841 aactggtaaa taaacttggt ccccgaagaa gccagttact ggctaatgct ttctggcagt
     3901 ataatgaatg tacggaattg gaggatattg ctgtctttaa gcgggtagct tctttacctg
     3961 catatattct tcagttcctg gagagccgcc ccggttttcg ttatgcagac tccttgttag
     4021 tgcttgtagc ggctcattac ccttcaaagg ttgtttctta cctgcttcag cataagcccg
     4081 gattacagtt acttatccgg atgaataaaa atatttactt gcaacagata gtatctcttt
     4141 cggaaaaccg gtttatatca gaactactac cttttattat ccccttagca gaaaaaagaa
     4201 ttacagttac ggagatactt gacaaaagaa aggatccaaa gacttatttt gaattgctgg
     4261 ttaatacttt aaagcatgag ttgtcaagac ctgcaattgc ttcttcaatc tttcttattc
     4321 cattaaggaa tggcataaaa gataaatcca tttcttttta tgtgaaccaa ataaatgaat
     4381 tacatagttc tgctgctgag atccgctttg ctgccgtgaa tgaccttagg gttgaggatc
     4441 tttattatat aataaccagt tgcgaagaag aactatatac ttccagctac ctgggtttgt
     4501 acaggaggct tatggaacat tttaataccc cggctgttga ttcactattc ggtattgtga
     4561 agtatgataa tttccggccc tttatgcgca tggcagcgat ttataataca ttgggcgatt
     4621 ttttaagttg tatgccgcag aaaacagccg ctggtttatt gaaacgtttt atctcaggaa
     4681 ttgaaactga tgcaaataca gggcttgcaa aagctatgga tatagcagat gctttttccg
     4741 ggtttactgc tttacctggt atcagcttaa tgatccaaca ggagcttaag tccaattatg
     4801 accgttgcaa gtatgaccaa tcattttttg ggatgcgtct ctacagtatt ttgcagaatg
     4861 tttttgacct ggtacagaag aaagactccg taaataaatt ttgggccaac ctgggaaacc
     4921 atgaattact ggaacgtaag gttctgcaga ataaacggga tgaaatcatc gaggtggttt
     4981 tattttatgg cgatgaggat ggtattggtt ccttcaataa tttcatcagg ctgtacaagg
     5041 atcccaccag gtggaaaatc tcaaaaaata actattggat aactatacgt tctctttctg
     5101 aacaacctgt tattatttac gcaaacctgc cacttgataa tgaaacagaa atggatatgc
     5161 ttgcacagga ttctttgttt gcattcttaa aacagcaaat ggccgaaccg gttatcctga
     5221 ttcaccgggg tcatagttat catcttagcc atacattaag aaggctacag ccaactgtta
     5281 aactggcaat ccttggctcc tgcgggggtt ataatagtct gttaaacatt gctaatatca
     5341 gtcccgatgc tcaaattatt gtttcaaaga aaattggttc gcaatttatt aatgatccaa
     5401 tgattgatgt gattaatgag aacttacaaa ataacagaga tcttgtttgg tcagaaatat
     5461 gggagaagct acgaatccgg ttcagtaaga atgagctgat gcttaatctt ttcaatgaat
     5521 atattccgcc tacaaagaat gtaagtctct ttgtgcttaa actttttaat ttctacaggt
     5581 aaacgttttt aactgaacgc tggctggcct acctggtttt cccggcttta ttttgcaata
     5641 gtgccaaata agtcaccctt attccattct ggcctcagca acgtttgtgc gatggaaaag
     5701 ctaaccagga aatttaactg aacataggtt tttgcagccg caaaattgaa aatgccatcc
     5761 atgccatcag ccgattgatg ataatatttt gctctccact ttttaacata ggcatccata
     5821 tcaaaccccg gagtatcgga cttattgcca tatttgatat gcagggcagg aataccattc
     5881 atgacaaaac tatactggtc actccggaca aaacgatttt cgtttggttc cggatctttt
     5941 tcaatatcca gtccaagata ggaggcggcg aattttacat tgttcataat acttgaatgt
     6001 tcagcaccca acggtacaat agaaaggaga ggagcaataa cagtaggcat atcggtattt
     6061 acattggcaa cgatggagcc ttttggcact gtaggattgg ctgcgaaata agaggacccg
     6121 atcaatccca tttcttcgcc tgtgaccatt gcaattaata tcgaacgttt tggttttacc
     6181 cctgatttgt atatccttgc aatttccaac aaggaagcta caccggaagc attgtcatgg
     6241 gctccgttgt aaatagaatc cccgtttaca actctgccaa tccctacatg atcaagatgt
     6301 gccgaatgaa ctacatattc atttttcaaa accttatccg acccgggaat taatcctacc
     6361 acattataac tttcaaaatc attgtgagtg gttttgtaag atgctgaaat gctgtacggc
     6421 aactcaaaag aagaaggatt cccttttttt attcctgcca atacctgctc aagatttttc
     6481 cccgaattta aaaacaaacg ggtgagtatt tttttattgc ttataagggc aattttaaga
     6541 ttgcctgtaa atacacgacc aaatgctgca gatttatccg cattaagtgc cacgttgaat
     6601 tgtagcaccg gattgggatt ggagccaggt cttgcattca ggttaataat gataacacct
     6661 gccgcacccc tggcataagc agtgttcatt ttattacccg gacttgagaa atgagctgta
     6721 aatgtggagg ataacccgtc cggagctcca tttattataa caacgatctt gcctttgaca
     6781 tccattccgg tatagtctga atatccaccg ggaatatcaa tgccataacc actaaatatc
     6841 aattgtcctt cggccgatgc cattacacta aacgggtgcg ggaccggggt aaaatcattt
     6901 tggaagataa gggaatctat atttccttct gaatctttta agacagcaat ggaagagcta
     6961 ttattgacga tcgatttcct gagaatcact ttttgtgtaa agccaccatt atccccgccg
     7021 ggagttactc caatttgcct gtattggtca atcacataat caacagccag ctgataacct
     7081 gctgtaccgg gaaggcggcc ttttaatctg tcatgagcaa gataagcaat gtgagaacgg
     7141 atgttgtttg tatcaatcct gctcatagcc tgttttatgc tctttgagat ttttacatcc
     7201 tgtgcaaaaa ttggcagggt ggcaagacta caaataaaaa taaaatatct tttcatgccg
     7261 aaaagataaa caaatcaggg cagaataaat tgaacacgac gattacattt tcctctattc
     7321 ttgtaataga tttgttcagt attcctgttt ctataaattg taagtgaatt aaagcagttt
     7381 tgccctgtta aagtgaaaaa ttaccttttc aaaatatttt gccttttatt attttcatac
     7441 tgtcacatct ttcgtaattt tctgtaaatt attttttgca gacaaagttt tatataccgc
     7501 atctgctgtt gcaggagttt gtccaatgca tcatgatagt acatgctgaa ttggataaga
     7561 atgctgcccc tgttatttgt ccttatccgc ctactcctca aaattccctg ttcttttata
     7621 tcaatgatcc gataaaagta cagatcgaag ggaaaaagga ttttctgttg cagccaaaaa
     7681 gtgttgtagt agggcaacaa cttacccggg ttatgctgga tatcaataaa agtcataaag
     7741 cggtaagggt aggatttcat cccggtggat tacaccgcct tatgggattg tcaatggcgg
     7801 atatgattga cggaaattat gatgcagctg atatttttgg gaatgaaata caggaagtaa
     7861 ataatcaact gcaggaagca gaagattttg atgctatcag gtcaattgtt gaacggtttc
     7921 tgttacaaaa agttaaaaaa ctgaaacagg ctttaccatt tgataaagcc atgttagagt
     7981 tactgcgatt gaatggaaac gtatcaatcg aaaagattgc ttcgctggcc tgtttaagtc
     8041 ttcgccagtt tgaacgggtc agtaaagaaa gaattggatt acctcccaag ttatttgcac
     8101 ggctgattcg tttttccaaa gcttaccgga tgcgggaaaa ttttccactg ttaagctgga
     8161 ctcagattgc ctatgaatgt ggatatttcg atcagatgca ttttatccgt gatttcaagc
     8221 aatttgccgg tgtggcaccg ggcatacttg aaaaagaact ggaaaatacg cctgtccgac
     8281 tgcaggcaga tctgcggttg taattgtcgt ttttatacta tttatccatt gttgctgttt
     8341 cgaattttgt attaaaatca gcaactatgc aacagtactc ctttctcttt tacctggcct
     8401 ttaccttatt tatgcttcat ccggcaaagg cccagcaatt tgataaactc aatgaattac
     8461 aagatcattc agtgaaagtg tattacagtg ccggtcatga acaaagagct tcgtcaattg
     8521 caaatcgtgt tgacaaagca tttaactatc aacagcaaca actggggttc aaaccttcgg
     8581 ttatattgct ggtactgagt atttccgact ggagcaacta tacttctttc cctgtgtatg
     8641 gtatgccgca ctacaacgat gataaaatac tgatcgtggc agctgaagac aatccattct
     8701 ggaaaagttt tttgcctcct ttggagcagt taccacctgc attaagtgaa cagatccaaa
     8761 ccgtatatcg ccggagtgat ggaagtataa gtatggaagc cttttttgac ctgctggcgc
     8821 tgcatgagtt agggcatgct tttcatttcc agggtggact aacgatgcaa cgcaaatgga
     8881 tgggcgaact gtttgcgaat atcttattac acacttatgt tgcagaaaat gagcccgaac
     8941 aattacctgc gctgacattg tttccccgca tggtgataag tggcggtacc aaagagttca
     9001 cttataccag tttgaaagct attgaagaga gatacgatga gattggtcag cggcatccaa
     9061 aaaattatgg atggtatcaa tcccgctggc atgccgctgc cggtaatatc tatgatgcaa
     9121 gtgggaaatc aatctgcagc aagttgtggg aggcgctaaa aaacaataaa gaagttttga
     9181 cagatgaaga attactgcgt tttttaggaa aaattcatac aagtatttcg gatatgatga
     9241 agaattggga cagtaatacc gtccggtaaa accggaagat cggtttgttt atagtaatca
     9301 gcattgcccc gctctaacag ttgaattttt gtttgaaccg aattttcatt ccggtaattt
     9361 gttctattat gcagtaatga accctatttt gaacagggta aatagccgct gataatcata
     9421 ctgggacatg cagaaaatca tacactgaca tgaacgggtc gtttactttt atttaaactc
     9481 gttttgttat gtctgtatgt ttttaccggt acacggttcc tgtttcatat acgaccggca
     9541 agatgtatcc caggagtcta tttttctcca gaaaaatttt aaaactattt ctatttgcat
     9601 tattcatatc aacctataca ttttcgcagg gttctcctga gtatggctcg ggtatcaaac
     9661 tgaatttaag ccctgaagga aaccggtata tacgtttcat ttcctggaac caggtatggt
     9721 ttcgtagcca gcaaaataat cccgggtcat tgattaattg ggaagtaaaa aataatacct
     9781 gggatattgg agccaggcgg gttcgaatga tagcctatgc tcaactgtca ccccgctatc
     9841 tgatcctggc tcattggggt ataaacaatc aaactttcgc cggcggtggt ggattcggct
     9901 cctcaggtac cggcccttca ggcgcaggta aaaaaccaca actatttttt catgatatat
     9961 ggaatgaata tgcgatcgta cccgccaaaa atcctaaaac aaatacatcc aataaaaata
    10021 cattgtacct cggcgcaggt ttgcattatt ggttaggcat cagccggatg acgagtgcaa
    10081 gcacccttaa ttttatgaca attgatgctc ccatattcaa ctggccaatg attgaagtaa
    10141 gcgaccagtt tatgcggcag ttcgggtttt atgtaaaagg gaaattaggt aagcttaatt
    10201 actctgcatc ggtaaacaag ccttttgcaa ctaatagtac ccctttttat gattcattga
    10261 aaggccgggt agccattgac aataatggtg acgccaaacc ctctgtacag ggatatgcag
    10321 attaccagtt cctggaccag gaagccaatg tgttaccctt ccgggtaggc acatatgtgg
    10381 gaacaaaaaa ggtattcaat atcggcgccg gtttttatca taatactgat gctacgaaaa
    10441 gtactgacct gggcgggaca ataaaaaaac acaatatcaa cttactgggt attgatttgt
    10501 ttgctgattt acctgtgggt aataaagcaa aaaacatggc aataactgca tacagtgttt
    10561 tttataatta caattttggt ccgaattact ggcgaacatt aggtgtaatg aatacatcca
    10621 cgggatttga tcctatgctt ccggttgcca accgcgccat taacggaccc ggcaatgccc
    10681 ggatgttcat aggaaccggt aatatttttt atacccaggc tggattgctg ttaccaaagg
    10741 gcaaaagcaa caaattgaga gtgcagcctt ttggggctta tacctggaaa aaatttgatt
    10801 atttaggtga accaggaaat tattttgatg taggggccaa ttttttcatt gacgggcata
    10861 atgcgaagat cacaccacag tatagtaccc gaccacttta ttatttacgt aatggtcagc
    10921 atataaagga cggtacaaaa ggtgaatttt taatacaatt ccaggtctac ctgtgatgaa
    10981 agctttttct gaccgacagc aaaactatag catcatttag ttgaattttc attcaaatgg
    11041 tgtttctcat gtacctgttt gatggataaa ttacagatta atccaatcac cagtaatgca
    11101 gccattacat acatagttgt ggaatatacc tgcgccttag gcagtttgct tacctcaatc
    11161 tggtactggc gcatatagtt tattagtaca ggcccgagta tcgcagccat gctccatgcc
    11221 agcaacaacc tgccatggat agccccaacc tgcttagtgc caaataagtc tctcaggtag
    11281 gcagggactg tggcaaaacc accaccatac atactaatga taatcgcaaa agttgcgaca
    11341 aacattacta cagatcctga tgcaccgatt gaagggatgg atgaatataa aataatgcct
    11401 aaggagaaaa agattgtgta tgtatttttt ctgccgataa tatcagacag ggaagaccag
    11461 atgaagcgac ccgccatatt gaataaactg agcagcatta caaatccggc tgctgcggaa
    11521 gcatcaaccg ctttggaagc acctacagat tctacggaaa ataattcctg tatcataaca
    11581 gaggcctggc ccaatacgcc gatccccgcc gttacgttaa gcatcagcac catgaagagt
    11641 aaataaaatt gtggcgtctt tactgcattg tcaaccaaaa catgggcatt ggtgaccagc
    11701 ttttttgcca tggcttttgc aggatcaaaa cctttgggct tccagtcctc agccggtact
    11761 cttacgataa cagcaccaaa catcattatt ataaaataca ggcaacccat tactaaaaaa
    11821 gtttgtgcca cacccaccga ggtctcattg ctgaattgtt tcatcaggta aacggacagg
    11881 ggagatgcga tcattccgcc tccgccaaaa cccataatag ccataccggt agccatgccg
    11941 ggtctgtcag gaaaccattt tatcaatgta gaaacaggag aaatataacc taagcctaat
    12001 cctatgccgc caatgacacc ggagcccagg tataataacc agatctggtg taagtggata
    12061 cccaatgagg agatcatgaa tccacaggca aaacaaagtg ctgaagcaag cattgccatt
    12121 cgggggccca ctctttccat ccattttcca aatgtggctg ctgaggcacc taaaaagaaa
    12181 agggcaatac tgaagatcca tccaagagta gttagtttcc agtctccagg gactgattca
    12241 gtgatgccaa tgacctttgt gagtggctgg ttaaatacac tgaaagcata gatttgacca
    12301 atgcacaagt gaatagataa agcagcaatg ggtactttcc aacggttgta accgggagga
    12361 gctatggttt tttcccttga aaagaatgac attcagcaaa ttttattgct gcaatataga
    12421 tacagtgaat atagagaaag atgatttttt tcaggtaaaa cgtaggtttt agtcagttgt
    12481 tatgaggaga tgcagactat tatttaccac ggggcaagtt caagctttat aattcagggc
    12541 aaagcttgcc aaaatactat aaaaaaagca gacttcagca atacttaccg aagtcggggc
    12601 aaaaatttga gcttgcgtga tggctattca gttcgttgtg tccggaatta gaatatttaa
    12661 aaatgcttta aagctatagt taaagtattt tctttcggac atcggtttcc attctatttt
    12721 gttctgtttt attattattg atccagatag gttcttccgg tggcattttg tcggtgcaat
    12781 caataataag cccattggct tttccaagtt ttgattttag tatagcatct ccttcgctaa
    12841 tactgttata tttttttaga atccagttat gcagtatggt aggctcatat aaaattaatt
    12901 gcctgcaggg ttgtttttca gggtgtgccc actgctttga tagcaataac ttaaaaaatt
    12961 gcacatgttt gtctttttct gagttgccgc cgattagttt ttcgggaact tgcactttaa
    13021 cagcccattc gttagaaggc aatacctctt ctaagccacc tacttccaaa aaactgtgtg
    13081 caaaagctga acaacccgct cccagtcctt ctcttggttt attaaacccg ttgtataatc
    13141 tatcataacc ttttagctta tactcctgca ggtattgcca taacctgtta aaaacggttt
    13201 gattgatcct gtattttata aaagtgatat ctcccgtttc gcagcgttgc tttaattggg
    13261 gcagatttac atactcctca tccagcctgc cgttgatgat ttcaaataat attcccaatc
    13321 catagccttt tctgattact ttctttgtca tacctgaatg agcctgagag gtagtgccca
    13381 ctaaggtata cctggaactg tctttcatct caacgatcat atggccaatg ggatgttttt
    13441 ccttcccata gcgggatctt gcaaatgtat tattgatata actaaacact aatttccggg
    13501 gagaatccca gtttatacct tttggagaag ggatagagta tattgtaagc tcctgtgtat
    13561 aagtcaggcc agccaaaaat acagtcagca gcagcattac tgcctggctg attaataatt
    13621 tcgggtacac ctgttgcagg ttattatttt tagattataa aatatactaa cagagctttc
    13681 gtccgatata taagtataag aacaccttcg atgcagccga aactgattta gcaataacta
    13741 acttcttttt ttaatgtaat taatgttgag aatgtttctt gccaaaagtc cttacataat
    13801 tcacaatgtc accaggtata cgggttttcc caaaactacc ctccattgca tcctggtttc
    13861 cgggtatcct tttcttaaat cccggcattt ctttatggcc ggaatagatt ttatagagta
    13921 aagcaccatc tgtttgttgc tgaaatgcag ctgttgtaaa gtcagtaggc gtattgtcaa
    13981 attttaaagc tctttttcct gagccttttc catctgttcc atgacaggaa tgacaaaaat
    14041 agtcatagag ctccttgccg gaagcaatag aagattcatc tgctactacc gggtttttta
    14101 atttttcata tttctcggga actttccagg gatcttcctg ctgtacgatc ataaataaaa
    14161 gtgtggttgt aattaatgca acaacaccca gcaataaaaa tgatcttttc atattattaa
    14221 tttgagtggt cattaaaatt caaaataaat cttcgtttaa atataggcgc aattgcctga
    14281 cataaattat gacgccagtc actatatacc atgtgtttaa tcacaagtaa ctacttagaa
    14341 taatgaattg aaaaaatacc tccgtatctt ctttactgct tttgggtgac caataaacag
    14401 gacgagattg aaaagttcta tgtttatggc tttcctagta attgctgcat ataccgaagc
    14461 cctttttccc ttgtaaatgc gatgttccgg tttggaacct catcaatgtc cgggtaagag
    14521 tcaatggctt tactcagact ttcttcccgt aaaaaatgta acatcgggta gggggaacgg
    14581 tttgtataat tggaggggtc gttttcgttg ctccctgcaa acaaatactt agggtgaaag
    14641 ctggcgatct ggtaaatacc ttcatatttt tctttcacca gtaatttttc agccagttcc
    14701 agcaggtgaa gatattcttc gaacaattca aagctgccgg ggaatattaa caatgaggtt
    14761 tcgatatgtt tagcagcatc cagttgatac atagtagtca ttacagcctg taatgctgtg
    14821 gtcgtggtgg catcagttaa tacttcatac tgtatagtgt cccgctttat ttctttggcg
    14881 gcaaatggac aaaaattaca gcctatcacc acatcaatga tccatttctt tgtttgttga
    14941 atgatggtcg ctttatccgg gtagttcatg ctaattcttt aggtaaagta tctgatatac
    15001 aattcttcaa ctttctttct tgcccaggga gtcttgcgta aaaatttcaa tgaagaattt
    15061 atacttgggt tactagtaaa acaattgatt tttatgatag ttcccatttt ttcccaaccg
    15121 aaatgatgca ctaattcttt aagaaccatt tccagggttt ttccatgaag tggatcgttg
    15181 gagtgcataa aagttagttt gatgtaaaga taaccctgta tggccggtct tcaatcgttg
    15241 gaaggattca aattttagat cgcaaaacca gtgagaactg tttagctaat gctaaaacaa
    15301 tgaaattttc ttttcattaa ggtctcaatt agtaagctgt atttaatgtg tgatatcccg
    15361 attgttggat gataatagca ataacacaac tgttcagttt agaattggga aagaaaataa
    15421 gtagatttat agcgaataaa catttttaga tgcaatcact ccgggaaaaa aacattgcca
    15481 ttttatgcaa tgccctggcc ggatccggga gcgccgtttt gtgtgcagaa aatatttgta
    15541 ccaggttgtc tgacaaaaat attcgacata cccaattcaa agatacctgg cctgatgatt
    15601 tcagccttta tactgatatt tttatcgttg gtggagatgg tacacttaat tattttatta
    15661 accgataccc gggtatgagc ctgcctttag tgatttttaa aggaggcacc ggaaacgatg
    15721 tttactggtt actgtatggt aatagcacat tggatgaaca attggaaact gcattgaact
    15781 catctccaaa gccattggat atcggaaagt gtaatgatcg ctattttatt aatggagttg
    15841 gaatcggctt tgaaggagaa gtggccaaag ccctgaccgg taagaaaaag ctgcccggca
    15901 aaacttcttt tcttattact gttctgaaaa agatagttac ttatcgttca aaacgctata
    15961 ctatccaatc agctgaacgg agcctttcag gaaggaggct gcttgttgat atcagtaatg
    16021 gacgtcgagc cgggggtgga tttcatatag cccctgaagc ccgggccgat gatggtttat
    16081 tggatgttat tatcgctgat gcactcactc ctcttcagcg ggttcgttat cttccggtca
    16141 tagaaaaagg gaaacacctg catttgtcat tcatccatca tttcagaaca aggaaaatca
    16201 gtattgaagg tgatgacctt ttgcaatatc acctcgatgg agaatatttt gttgctaaaa
    16261 gactgaatat tgaaattatt cctgctgcag ttacctttcg gttttaaatc aaccggtata
    16321 tcctttttaa atgtggtagt tgaaaaactc ctgatctgtt ttcctctatt gtattgacaa
    16381 catcccttat tttagtgata tgaaagcggc gactatcaat gaattgaagc aggaattact
    16441 aaatgcttct tcggcaaggt taacggaact ttgtctgcgg ttggccaggt ttaaaaaaga
    16501 gaataaagaa ttactgactt atttactttt tgaagctcac gatacagatg cttatattac
    16561 cagcgtaaag gaggaaatgg ccttacaatt tgatgccata aatagaagca atgtttattt
    16621 tgtgaaaaag acactccgga agatcttacg tatcgctaat aaattcatcc gttacagtgc
    16681 atcagcaata gtggaaacgg aattgctcat tttcttctgt ttgaagttaa aagcaatggg
    16741 tgactcaata aacaaaaacc ctgtaatttt aaacattttt caaaatcaat taaagaagat
    16801 caataaatca atttcagcaa tgcatgagga tctgcagtat gattatcaga gggaagtaag
    16861 agcactaact gtttagaaaa gatttgcaag caaaaattta taaacgatct ttcttacatc
    16921 tcaaactccc atactgttct gtatgttcca tgtttttcta tgtaggtcag gaaggaatta
    16981 tagaaaacat cagcaccaat agtagcactg tcaccaatga caaatagttg ttcttttgcc
    17041 cgggtaatgg ccacattcat ccggcggtag tcttttaaaa acccaatatc accatcatcg
    17101 ttacttctta caagcgatac aataataatc tctttttcct gtccctggaa actgtcaata
    17161 gtactcaggc gaagttgttt tggcaacatt tctttagcag cagctacttg tccggagtaa
    17221 ggtgagataa aagctgtagt taaaggattc aatgactctg tttcgattat tttttgaaca
    17281 atacgtaact cgccttcatt ttgtaaactg gtaccatcag gtccatgttc ttcgttgtaa
    17341 ccagacccgg cagtatcaat aaatgttaga tggacaccgg tattgtataa atgttcagca
    17401 gttaataata agctgttata aaaataatta ctgctgaacc ctgcaatagc ctgccgcatc
    17461 cggtattggg tgttgagcag aaagacatta tttgttttga taatcgctct ttctaaaatg
    17521 gattcgttga aacccatatt agcagcctca ttgcttaata cggtaggggg cagttgccag
    17581 tggtctcc
//
LOCUS       DAORTI010000068        17545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2899_length_17545_cov_7.823213, whole genome shotgun sequence.
ACCESSION   DAORTI010000068 DAORTI010000000
VERSION     DAORTI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17545
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_17545_cov_7.823213"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLO70_09745"
     CDS             <1..807
                     /locus_tag="PLO70_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74791.1"
                     /translation="NATNSGTGSSLTLGDYNLVMDTASLITNSTNYIITNGTGRLVRR
                     VENGSTVLFPVGVDEDSYTPATITNTGAADDFKVGVNQGVLSAGSTPAPVLTGNVKRT
                     WSVEKETAGAGSVNLTFQWRLADEQPGFNRATSYIAHKLSCPPPPPPNCDATYYDAVA
                     TTAATGNDPYIQFRAGVTSFNTPSFIVTSQEVVYRFINVYGNTPGDGNWSNPLNWTNA
                     QAPPSIIRPGMSVIIDPLIGNECIFTGPLIVQPGGKITVSEGKKLTILSQ"
     gene            1231..2241
                     /locus_tag="PLO70_09750"
     CDS             1231..2241
                     /locus_tag="PLO70_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQZ74792.1"
                     /translation="MAKTIDLKIHSAQQNLRVQKWVAALSVIILAIKFIAYYSTHSIA
                     ILTDALESIVNVAAGFIGLYSLYVAAKPRDFDHPYGHGKAEFISAAIEGTLISSAGAI
                     ILYKAVQNLIHPVELQKLDIGIWLVAVTAVANFAIGYFCLQTGKRNNSLALIASGRHL
                     QSDTYSTIGIIAGLLLLYFTKLKWIDSVVAILFGVIIIITGYKIMRRSIAGIMDEADL
                     ILLSKMVEVLNANRKENWVDLHNLRVIKYGSILHVDCHFTVPWFLNVHEAHQEIDELA
                     ALIRKEFGESLELFVHSDGCLPFSCRICKKANCPERKNNFAQEIKWTLENISKNKKHE
                     LI"
     gene            2238..2630
                     /locus_tag="PLO70_09755"
     CDS             2238..2630
                     /locus_tag="PLO70_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQZ74793.1"
                     /translation="MKTVTNWKKNHEFESKLKHNSIKLDGDKENGFGPKALLLSGLAG
                     CSGIDVVDILGKMKVEFSKFSIEAEAEQTEEHPKVFKEILVTYTMKTAADNEEKVRKA
                     IELSLEKYCGVAAMLRKNSAIQYKLVIE"
     gene            2645..3052
                     /locus_tag="PLO70_09760"
     CDS             2645..3052
                     /locus_tag="PLO70_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQZ74794.1"
                     /translation="MLSKKSQYAFKALAYLTEKYKQGPVLIAEISKKKKIPLKFLENI
                     LLELKKADILDSKKGKGGGYFLKMDPVKVKVATIVRLVNGPIAMLPCVSLYFYERCKN
                     CDEKQCGLHDMMIEVRDASLAILENRTLSDLVH"
     gene            3153..>3511
                     /locus_tag="PLO70_09765"
     CDS             3153..>3511
                     /locus_tag="PLO70_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQZ74795.1"
                     /translation="MGLHLGDIAPNFKAKTTAGDIDFHEYLGDSWGILFSHPADFTPV
                     CTTELGRTALLQEEFKKRNVKVLAVSVDPLESHQSWVKDINETQHCNLDFPIIADPDR
                     VVAFLYDMIHPKASETFT"
     assembly_gap    3512..3611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3612..4231
                     /locus_tag="PLO70_09770"
     CDS             <3612..4231
                     /locus_tag="PLO70_09770"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQZ74796.1"
                     /translation="QVVFSSSLGQEDQVITDAIFKNNIPVTVFTIDTGRLFNETYELL
                     DKTIARYKQSVKVYFPDAADVEAFVLQKGINSFYESVDNRKECCHIRKVKPLNRALAG
                     AKVWVTGLRSEQSANRNQMPMIEWDEGRQLYKFNPLINWTYGEVLDYLNVNNVPYNSL
                     HDKGFISIGCAPCTRAIGQGEDARAGRWWWESSQKECGLHVAVSK"
     gene            4285..5193
                     /gene="cysD"
                     /locus_tag="PLO70_09775"
     CDS             4285..5193
                     /gene="cysD"
                     /locus_tag="PLO70_09775"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQZ74797.1"
                     /translation="MNEYSLNYLEQLEAESIHIFREVAAQFERPALLFSGGKDSITMV
                     HLAKKAFAPGKIPFPLVHIDTGHNFPEALDFRDWLVKEVGALLTVRKVEDTIRERNLT
                     EPKGKFPSRNWLQTYTLLDTIEEFKFDCCIGGARRDEEKARAKERIFSVRDEFGQWDP
                     KLQRPELWNIFNGRIHKGENVRVFPISNWTELDIWNYIRRENIDLPSIYFAHDREVIA
                     HEGQLVAMSDYVQPSVDDTILTKKVRYRTVGDMTCTAAVESTASTLDEVINEIISTRI
                     SERGETRIDDKVTEAAMEDRKKNGYF"
     gene            5224..6465
                     /locus_tag="PLO70_09780"
     CDS             5224..6465
                     /locus_tag="PLO70_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQZ74798.1"
                     /translation="MDLLRFITAGSVDDGKSTLIGRLLYDSKNILIDQLEALEKSTKN
                     RDDGSVDLALLTDGLRAEREQGITIDVAYRYFTTPKRKFIIADAPGHVQYTRNMITGA
                     SNASLIIILIDARLGVVEQTRRHSIIASLLKIPHIVVAVNKMDLVNYSQDVYNNILIE
                     YSKVAKQLGLKEVTYFPISALNGDNIVDRSTQFNWYDGKPLLEYLEEVQLDNDVNLTE
                     SRFQVQLVIRPQTEELHDYRGYAGKVISGVYKKGDKVTVLPQQIETTISKLETGGKEV
                     KEIFAPQPAVIHLAEDIDISRGDSIVRTDNLPQVKNEVEVLLCWMDEKPLQTGNKYLL
                     QHNSRLVKAIVKNVAYKLDVNSLQQQPVEGGVKLNEVVKATIKTASPLVYDSFEKLHV
                     NGSAILIDETSNSTVAAVMIQ"
     gene            6474..7508
                     /locus_tag="PLO70_09785"
     CDS             6474..7508
                     /locus_tag="PLO70_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQZ74799.1"
                     /translation="MIYTENEIKEVASISKSSPVAKIAVAEVITIDTDEVKEEESVPE
                     IEQDIIRDEKKWKWIATVSLLVFAIAILSFFILRYVGYAPLASLIQSAKSNIDKSFYL
                     FMLAGFLAQMVDGAVGMGYGVTSTSLLMSMGVPPAAISGSVHTAEIFSSAASGYSHYK
                     FGNVNKKLFKVLVIPGVLGAVAGAGMLVWMGDKYGNLIKPFLAAYCMILGFRILYQAY
                     KRNKKNKKVKKAGWLAGAGGFLDSFGGGGWGPLVTSTLISKGRSPQYVIGSVSLSEFF
                     VTLSSAMSFFVFIGITHWQVIMGLMIGGAVAAPLAAKLAGKLPVRTMLICVGLMVIIW
                     SIRVLVKFLV"
     gene            7585..8760
                     /locus_tag="PLO70_09790"
     CDS             7585..8760
                     /locus_tag="PLO70_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ74800.1"
                     /translation="MAKQRPDTKAIRIQTKKTAQKEHSTPLFLTSSFTYDSAEDMAAA
                     FTDDSLEVNIYSRFSNPTVDEFITKIAALEGAEDGVATGTGMAAIFATFMTFLSKGDH
                     IISASAVFGSTHTILTKYLPKWGIEYSYFDMNKPETITALIKSTTRMLYVETPSNPGL
                     DIIDIEFVAALCKQHHILLVVDNCFATPAVQQPIKYGADLVLHSATKFIDGQGRVLGG
                     VVVGKKELVRQLYLFVRNTGPSMSPFNAWVLSKSLETLYIRMDRHAASAFELAKQLQG
                     HAKISAVKYPFLDTHPQYVIAKKQMSNGGGIVTFELKGGIESGRKFLNALKMISMTNN
                     LGDSRTIASHPASTTHSKLTEQERQAVGITPGLIRISAGLENVDDILADIFQALDKS"
     gene            complement(8765..9487)
                     /locus_tag="PLO70_09795"
     CDS             complement(8765..9487)
                     /locus_tag="PLO70_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQZ74801.1"
                     /translation="MKITDWIISETENWVAVNKPSGLLSIPDREGKEISLKVLLKEKY
                     GNIFTVHRIDRDTSGLIVFAKNEAAHKHLCKQFEDRQTKKIYTGLVIGSPANKKGSID
                     SPIVEHPALNGTMIIHRKGKESLTDYEVLEDFGIYSLLQFQIHTGRTHQIRVHMKEIG
                     HPIVADNIYGDGKPILISALKKKYKLSKDELEERPILGRLALHAFQLVFKDVDGTMIE
                     LEAPHHKDMRATLQQLAKRKSK"
     gene            complement(9518..10465)
                     /locus_tag="PLO70_09800"
     CDS             complement(9518..10465)
                     /locus_tag="PLO70_09800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQZ74802.1"
                     /translation="MNKKASNNFGKFAKKKSNAAVKEQFKQEKRKVKKEREEYFDKKK
                     AEARGKVTIQSRQSTAGGQHSPVSHSRPETTGTMPLNKFIAHAGVAARRDAAEMVRKG
                     LVKVNNELVTEPGFKVSAKDEVRVNGKKIFLAKNLVYILLNKPKDYITTTDDPQGRKT
                     VLDIIGRATPERVYPVGRLDRNTSGVLLLTNDGELAQKLTHPSNEVKKVYHVTLNKPL
                     DKKDFEQILKGVALEDGAANVDVLAYADAKDRTQVGVEIHSGRNRIVRRIFESLGYDV
                     RNLDRVIFAGLTKKNIERGKWRFLSEKEVRDLKHFGKGK"
     gene            complement(10577..11251)
                     /locus_tag="PLO70_09805"
     CDS             complement(10577..11251)
                     /locus_tag="PLO70_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74803.1"
                     /translation="MKKIIFSVLVASIAFTVNAQEIPERKGGRPMMHERQKGHHDRSM
                     AMKQLNLSEAQKEQFKVQKQSFQKQMEELKKNDNITVKEWKSKMETLRKDHKAKMDGI
                     LTSDQKAQLEKKKAEGKARQEEMMKQRSDKMKTQLGLTDEQSAKMDNSRKEIGAKMKA
                     IRENKSLSEEKKRAEMKELMKQQQESMKSILTEEQLKKMKEGKKHRPGPGMHRKGPAP
                     QDKETI"
     gene            complement(11398..12447)
                     /gene="rlmN"
                     /locus_tag="PLO70_09810"
     CDS             complement(11398..12447)
                     /gene="rlmN"
                     /locus_tag="PLO70_09810"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQZ74804.1"
                     /translation="MKKALKNIRHLTLAELEQYFEEMGEKKFRAKQVHEWLWQKQAMS
                     FADMTNLSKDLRQKLGDTFTLPALTIDATQYSSDGTIKSRFKTWDGHLVEGVLIPTKS
                     RFTACVSSQIGCSLSCKFCATGYIDRKRNLDFDEIYDQVVLINKQCETTHGKKLSNIV
                     FMGMGEPLLNYKNVLRAIERITSPSGLAMSPKRITVSTAGVAKGIRQLGDDKVKFKLA
                     LSLHAATDAKRHEIMPINDTNNIKTLIDALNYFYKQTGNEITFEYILFKNFNDSLQDA
                     DELIKIYRQVPADLINIIEYNPIEFARYEKPEEEKVQAFMQYLEKHRVNVRLRRSRGK
                     DIDAACGQLANKDSH"
     gene            12518..13711
                     /locus_tag="PLO70_09815"
     CDS             12518..13711
                     /locus_tag="PLO70_09815"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQZ74805.1"
                     /translation="MKSVYVIDSVRTPVGKYGGVLSSIRPDDLLAYVIKALLLRNSGI
                     DLNAIEDVIAGDANQAGEDNRNVARMAALLAGLPVSVGGNTVNRLCASGLQAIMDASR
                     QIMCGDAELIIACGVESMTRAPFVMPKSSDAYSRTTEIFDTTMGWRFINKNLSALYHP
                     YTMGETAENIAKQWKISRQEQDEFAFASQQKYAAAKAADKWADEIAAVELNNKGLISW
                     VVNDEHPRETSLDKLAALKPAFIKDGTVTAGNSSGINDGAAAMLLASEEAVRKYNLQP
                     MAKIVSMAVAGVDPAIMGIGPVPATQKALQRAGMTMADLDLVELNEAFASQSIACIRD
                     LGLDNLKVNVNGGAIAIGHPLGCSGVRISTTLVHEMRKRNAKFGLATMCIGVGQGAAV
                     IYQNV"
     gene            13775..14155
                     /locus_tag="PLO70_09820"
     CDS             13775..14155
                     /locus_tag="PLO70_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74806.1"
                     /translation="MKQFFLTLCVLLVFSAVNAQTDSLKQYTGKYIFPEGSPVTEIGV
                     VLENGLLTATSAMGNSELKNTDTKDVFEVVAYGGIATFKRSAEGKVNGVQIQVQDVNM
                     EGTKTEGLAYAELKVMLPVLRIRN"
     gene            complement(14215..15582)
                     /gene="hisS"
                     /locus_tag="PLO70_09825"
     CDS             complement(14215..15582)
                     /gene="hisS"
                     /locus_tag="PLO70_09825"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQZ74807.1"
                     /translation="MAKPSLPQGTRDFGPDVVRKRSYILNIIKSVFELYGFQPLETPA
                     MENIETLMGKYGEEGDKLIFKILNNGLDNPEKEKQIREDFEKVLQGKNVKGITERALK
                     YDLTIPFARYVAMNHSHLTFPFKRYQVQPVWRADRPQKGRYREFYQCDADVVGSNALL
                     NEAELASIYHEVFSRLKLDGYELRINHRKILTALAKTCGGIDKMMDITIAIDKLDKIG
                     IIKVKEELTQRGLTTEQVNIIEKYLNINGTNDEILNSLLEIIGKSEIGKKGIEQVSFV
                     LANLQPQHLNLKLDPTLARGLNYYTGIIFEAKAPAEVKMGSIGGGGRYDDLTGLFGVP
                     NIPGVGISFGVDRIYDVMEELNLFPPDVHIGTQVLFFNLGEAESTASFFLMQQLRQKG
                     VRCELYHESAKFDKQFKYAEKKNIPFVIILGEKELKESTCNIKNLTTGIQQTIPTADL
                     LKHQF"
     gene            complement(15592..16083)
                     /locus_tag="PLO70_09830"
     CDS             complement(15592..16083)
                     /locus_tag="PLO70_09830"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HQZ74808.1"
                     /translation="MKILMVCLGNICRSPIAEGVLQDKAFKAGLTWCVESAGTNSYHI
                     GEAPHPLSQKVAKQNGIDISSQRARRFKAEDFDQYDKIYALAEDVMDEIKRIARNKFD
                     AAKTDLLMNELYPEKNLDVPDPYYGPEKGYHEVYKMIDEVCDNIIEKYAIVNQQPADL
                     NNK"
     gene            16206..17024
                     /locus_tag="PLO70_09835"
     CDS             16206..17024
                     /locus_tag="PLO70_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQZ74809.1"
                     /translation="MTNKEILQASLLDIVFDKRNKAYGAYALRKGYNARLLTALAAGM
                     SVILLFIFINGMQGSKNSSFITSNKNEGIVIKQIELPKDKPAEPEKPKEVIKQKPVEK
                     IATAKYISNIKIEKDVKSAMTAIGDLDGKAIDSKEGDGKIDDGTEIVNVDPVAPVGNG
                     AIPAEPKQPDFVIQERNPEFPGGAEALKRFLSKNLDTPGELEAGEMKTVKIRFKVDKD
                     GSVNTFEIVTSAGNDFDHEVVRVCKKMPKWVPAIQNGVNVPVNYVLPVIFVGSE"
BASE COUNT         4947 a         3266 c         3882 g         5350 t
ORIGIN      
        1 aacgctacca attcaggtac cggttcatca cttacccttg gtgattacaa cctggtgatg
       61 gatacagcat cgctgataac aaacagtaca aactatatca ttaccaatgg tacaggaaga
      121 ctggtaaggc gggttgaaaa tggatccact gttttgtttc ctgttggagt tgatgaagac
      181 tcatatactc cggccacaat tactaacacc ggtgctgcag atgatttcaa agtgggtgtt
      241 aaccagggag tactaagtgc agggtctacc cccgctccgg tacttactgg taatgtaaaa
      301 agaacatggt cggttgagaa agaaacggct ggtgccggat ctgtaaacct tacctttcaa
      361 tggagactgg ccgatgaaca acccgggttt aaccgggcaa catcctatat agcgcataaa
      421 ttgtcatgcc ctcctcctcc tcctccaaac tgcgatgcca cctattatga tgctgtggct
      481 acaacagctg ctacagggaa tgatccatat atacagtttc gggctggtgt gacaagtttt
      541 aatacacctt cttttatagt tacatctcag gaagtcgttt acagatttat aaacgtttat
      601 gggaatacac ccggtgatgg gaattggagc aatccgctta actggacaaa tgcacaggca
      661 cccccgtcaa taatcaggcc tggtatgtct gttatcattg acccgttaat tggaaatgaa
      721 tgcatcttca caggtccgct tatcgtgcag ccaggaggta aaataactgt gagtgaggga
      781 aagaaattaa ccatactttc tcaataagag ggagtatagt taacaacatg tgtatcattt
      841 tttataaccg actgcatagc caaaggcttt caagtagtag cagagttctg tatactaatt
      901 tttaaataca atttatgttg cgtcataaat ttatctttta aatctataaa agatgtgatg
      961 agatttgcct gttacagatt agattttata actgctgaaa gaacaaaata ttagaacctg
     1021 gtcgtgaaat aaaaagtaac aagacgaatg ctgtatcata ttatagaggc agttctcatg
     1081 aagacagtaa ataatttttg gaacagcttt atgttttgtt acctgaagat gataaagctt
     1141 atttttaccc ggatttactg gattttcagt tagcgattac tggcttaatt cattttgcag
     1201 gttataatta tataagtagt aaaatataga gtggcaaaga cgattgattt aaaaatacat
     1261 tcagctcagc aaaatctcag ggtacagaaa tgggtggcag ccctttcggt aattatcctg
     1321 gccataaagt ttattgccta ttattctact cattcaattg ccatccttac agatgcgctg
     1381 gaaagcattg taaatgtggc agccggcttt ataggccttt atagcctcta tgtggcggcc
     1441 aaacccaggg attttgatca tccttacggg catggaaagg ctgaatttat atctgcagcg
     1501 atagaaggca ccctgataag cagtgccggg gcgatcattt tgtacaaagc cgttcagaac
     1561 ctgatccatc ctgttgagct gcaaaaactt gatataggga tctggctggt ggctgttact
     1621 gcagtagcca attttgctat aggttacttt tgcctccaaa ccgggaaacg aaataattca
     1681 cttgccctga tcgcaagcgg cagacacctg caaagtgata cttattccac gatcgggata
     1741 atagccggtt tgctattact ctactttaca aaattgaaat ggattgacag cgtcgtggct
     1801 attctttttg gtgttataat tattataacc gggtataaga tcatgcgtcg ctctattgct
     1861 ggcattatgg atgaggcaga cctgattctg ttgagcaaaa tggtggaagt attgaatgcc
     1921 aaccgcaaag aaaattgggt ggatctgcat aatctccggg taataaagta cggttctatt
     1981 ttgcatgtgg attgccattt tacagtgccc tggttcctga atgtgcatga agcccaccag
     2041 gagattgatg aactggctgc actgatccgt aaagaattcg gggagtcact tgagttattc
     2101 gttcattcgg acggatgtct tcctttttcc tgccggatct gtaaaaaagc aaactgtccg
     2161 gaaagaaaga ataattttgc acaggaaatt aagtggacac tggagaatat ttcaaagaat
     2221 aaaaaacacg aattaatatg aagacggtta caaactggaa gaaaaatcat gaatttgaaa
     2281 gtaaactgaa acataatagt ataaagctgg acggtgataa ggaaaatggt tttggtccca
     2341 aagcattatt gttgtctggt ttggccgggt gttccggaat tgatgtggtt gatatattgg
     2401 gtaaaatgaa agttgaattt tccaaatttt cgatcgaggc agaggcagaa cagacagaag
     2461 agcatcctaa ggtgttcaaa gaaatactgg ttacttacac aatgaaaacg gcagcggata
     2521 atgaagagaa agtcagaaag gcaattgagc tttcacttga aaaatattgc ggggtcgctg
     2581 ccatgctaag aaaaaattca gctatacagt acaaactggt tattgaataa tatttattca
     2641 gatcatgctc tccaagaaat cacaatatgc gtttaaagcc ctggcttatc ttacggagaa
     2701 gtataagcaa ggccctgtat tgatcgctga aatttcaaaa aagaagaaaa ttccgctgaa
     2761 attcctggag aatatcttac tggagttaaa aaaagcagat atacttgata gtaagaaagg
     2821 caaaggagga ggttattttc tgaagatgga tcctgtaaaa gtaaaagtgg ccaccattgt
     2881 caggttggtc aatgggccta ttgccatgct gccttgcgtt agtctttatt tttatgagcg
     2941 gtgtaagaac tgtgatgaaa agcaatgtgg tttgcatgat atgatgatag aagtaaggga
     3001 tgcttcactg gccatattgg agaacaggac attaagtgac ctggtccatt aatttgtccg
     3061 gggctaacag ttgtatgtta aaggtcttta agtctatcaa gttggtaggg aatctatttt
     3121 atagtagttt cgcaacttta aatggttaac atatgggatt gcatttagga gatattgcgc
     3181 caaactttaa agctaaaaca acggccgggg acattgattt ccatgaatac ctgggcgata
     3241 gctggggaat tttgttttct cacccggcag actttacgcc tgtatgtacc acagagttag
     3301 gcagaaccgc tttgctgcag gaggagttca aaaaaagaaa tgtcaaggta ctggcagtaa
     3361 gtgtggatcc attggaaagt caccagagct gggtaaagga tattaatgaa acgcagcatt
     3421 gtaatcttga ttttccaatc atagcagatc cggacagggt ggtagctttt ttatatgaca
     3481 tgatccatcc caaagcctct gaaacattta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn ngccaggtag tcttttcatc ttctttgggt caggaggacc aggtaataac
     3661 cgatgctatt tttaagaata atatcccggt tacagttttt actattgata caggccggtt
     3721 attcaatgaa acatatgaac tgcttgataa aactatagcc aggtataaac aatcggtcaa
     3781 agtttatttt cctgacgcag cagatgtgga agcatttgtg ctgcaaaaag gtattaatag
     3841 tttttatgaa tcagtagata accggaagga gtgctgtcat atccgtaaag ttaaaccact
     3901 gaaccgggca ttggccgggg ctaaggtatg ggtcaccgga ttgaggagtg aacaatcagc
     3961 caatcgtaac caaatgccga tgattgaatg ggatgagggc agacaattat ataaattcaa
     4021 tccgcttata aactggacgt atggtgaggt gctggattat ttaaatgtaa acaatgtgcc
     4081 ttacaattct ttgcatgata agggatttat cagcatagga tgtgctcctt gtacaagggc
     4141 tatcgggcaa ggtgaagatg cgagggccgg aagatggtgg tgggaaagct cacaaaagga
     4201 atgtgggttg catgtggctg taagcaagta atttttttgt caacatagtc tactggtaga
     4261 gtagacaatt atgaacttta aattatgaac gaatattcat tgaattacct ggaacaactg
     4321 gaagctgaaa gcattcatat ttttcgggaa gtggcggccc agtttgaaag accagcctta
     4381 ctttttagcg ggggaaagga ttccataaca atggttcacc tggccaaaaa agcatttgca
     4441 cccggtaaaa tcccttttcc attagttcat atagatacgg gacacaactt tcctgaggcg
     4501 ttggatttcc gtgactggct ggtaaaggaa gtgggggctt tgttgacggt gcgaaaagtg
     4561 gaagatacca tccgtgaaag gaatctgacg gagcctaaag gaaaattccc gagtcgtaac
     4621 tggttacaga cttatacatt gcttgataca atcgaagagt tcaaatttga ttgctgtatt
     4681 ggcggtgcca ggcgggatga agaaaaagca agagccaaag aaagaatttt ttctgtccgg
     4741 gatgaatttg gacaatggga tcctaaactt caacgacccg aactgtggaa tatattcaat
     4801 ggaagaattc acaaagggga gaatgtaaga gttttcccta tcagtaactg gaccgagctg
     4861 gatatctgga attatatccg ccgggagaat attgacttgc cctccatcta ctttgcacat
     4921 gaccgggaag tgatagcgca tgaaggtcaa ttagtggcga tgagtgatta tgttcaacct
     4981 tcggttgatg atacgatcct taccaaaaaa gtacgttatc gtaccgttgg cgacatgacc
     5041 tgcacggctg ctgttgaatc aaccgcctct acgcttgatg aagtgatcaa tgaaattatt
     5101 tctacccgga tcagtgaacg gggggaaaca agaattgatg ataaagtaac ggaagcagct
     5161 atggaggatc gcaaaaagaa tggctacttc tgatcaggtt tgaaacttat ttaaatatag
     5221 aaaatggatt tactacgatt tataacagcg ggtagtgtgg atgatggaaa aagcactttg
     5281 atcggccgtt tgctgtatga cagcaaaaat attctgatcg accagttgga agcattggaa
     5341 aaatctacaa agaacaggga tgacggttca gttgacctgg ccttattgac cgatggactg
     5401 cgggctgaaa gggaacaggg gattacaatt gatgtagcct atcgttactt tactacgcct
     5461 aaacgaaaat ttattattgc cgatgcaccc ggtcatgtgc agtatacaag aaatatgata
     5521 acgggtgcat ctaatgccag ccttattatc attctgattg atgccaggct gggtgtggta
     5581 gagcaaaccc gtcgtcattc tattattgct tcgctgttga aaattccgca tatagtagtg
     5641 gcggtaaata aaatggacct ggtaaattat tctcaggatg tgtataataa tatactgatt
     5701 gaatatagca aagtagcgaa acaattaggc ctgaaggagg tgacttactt tccgatcagt
     5761 gcactgaacg gtgataatat tgttgaccgg tccacacagt ttaattggta tgatggcaag
     5821 ccgttgctgg aatacctgga ggaagtgcag ctggataatg atgtcaatct tacagaaagc
     5881 aggttccagg tgcaattggt tatccggccg caaactgaag agctgcacga ttacaggggg
     5941 tatgcaggaa aagtgatcag tggagtgtat aagaaaggcg ataaagtaac cgtgttgcct
     6001 caacagatcg aaacgacaat ttcaaaattg gaaaccggtg gtaaagaggt gaaagaaatt
     6061 tttgctccgc aaccggcagt catacacctg gctgaagata tcgatatcag caggggtgac
     6121 tcgattgtaa gaaccgataa cctgccccag gttaaaaatg aagtagaggt attactttgc
     6181 tggatggatg aaaaaccatt acaaaccgga aacaaatatc tacttcagca taacagccgc
     6241 ctcgtgaaag caatcgtaaa aaatgtggca tacaagcttg atgtgaattc attgcaacag
     6301 caacctgtag aaggaggagt gaaactgaat gaagtagtaa aagcaactat taaaactgct
     6361 tcacctttgg tgtacgactc ttttgagaag cttcatgtta atggcagtgc cattttaatt
     6421 gatgaaacca gtaacagtac tgtagcggca gtaatgatac agtaaaggcc tttatgattt
     6481 atactgagaa tgaaataaaa gaagttgcgt caataagtaa gtcatcaccg gtggcaaaaa
     6541 ttgctgttgc tgaagtaata actattgata ccgatgaggt caaagaagag gaatctgtac
     6601 cggaaattga acaggatatt atccgtgatg aaaaaaaatg gaaatggatt gccactgttt
     6661 cccttcttgt ttttgccata gctatactct ctttttttat attaagatat gttgggtatg
     6721 caccacttgc ttcacttata caatctgcga aatcgaatat tgataaatca ttttatcttt
     6781 tcatgctggc cggattcctg gcacaaatgg tagatggagc agtaggaatg ggttatgggg
     6841 ttaccagtac cagtctgctg atgagcatgg gtgttcctcc cgctgcaatc agtggtagtg
     6901 tacataccgc ggagatattt agtagtgcag ccagtggtta cagtcattat aaatttggaa
     6961 atgtaaataa aaaacttttc aaagtgctgg ttatacccgg ggtgctggga gctgttgcgg
     7021 gtgcaggtat gctggtgtgg atgggggata aatatgggaa tttaattaaa ccattcctgg
     7081 ctgcgtattg tatgattttg ggttttagaa tattgtacca ggcctataag cgtaataaga
     7141 aaaacaaaaa agtaaaaaaa gctggttggc tggcgggtgc cggtggattc cttgacagct
     7201 ttggtggcgg cggctgggga ccattagtta caagtacttt gatctcgaaa ggccgcagtc
     7261 cccagtatgt aattggcagt gttagcctga gtgaattttt tgtcacctta tccagtgcta
     7321 tgtctttctt tgtttttatt gggattacac actggcaggt aataatgggt cttatgattg
     7381 gcggtgcggt ggcagctcca ttagccgcca agctggcggg taagctgcct gttagaacaa
     7441 tgctgatctg tgtaggtctg atggtcatta tctggagtat cagggtactg gttaaattcc
     7501 tggtttgaca aatcaggtct ggttccccat cttcatgctg ctgattgtat ctttgtttca
     7561 atactaattt ttcatacatc agcaatggca aagcagagac ccgatacaaa agcaatccgc
     7621 attcaaacta aaaaaacagc gcagaaagaa cattctacgc cgttgtttct taccagtagc
     7681 ttcacatacg actcagctga agatatggca gctgcattta ccgatgattc attggaagta
     7741 aatatttatt caagattttc taatccaaca gttgatgagt tcattactaa gattgcggcc
     7801 ttagaaggcg cagaagatgg tgtagccacc ggaacaggca tggctgccat ttttgcaacc
     7861 tttatgacct ttttaagtaa aggcgatcat atcatttctg catctgctgt atttggatct
     7921 acacatacta tccttacaaa gtatttacct aaatggggaa ttgaatattc ttattttgat
     7981 atgaataagc cggagaccat tacagcatta ataaagtcca ctaccaggat gctgtatgtg
     8041 gaaacgcctt ctaatcccgg actggacatc atcgatattg aattcgtagc tgctctttgc
     8101 aagcaacatc atattctttt ggtggttgat aattgttttg ccacaccggc tgtgcagcaa
     8161 ccaatcaagt atggtgctga tcttgttttg cattctgcta ccaaatttat tgatgggcag
     8221 ggcagggtat tggggggagt ggtggttggc aaaaaagaac tggttcgcca gctttatctt
     8281 tttgtacgga atacaggtcc atccatgagc ccttttaatg catgggtgtt gagcaaaagc
     8341 ctggagacct tatatatccg gatggataga catgctgcca gtgcatttga actggcaaag
     8401 caattgcagg ggcatgcaaa gatcagtgct gtaaaatatc ctttcctcga cacacatcca
     8461 cagtatgtaa ttgctaaaaa gcagatgagt aatggcgggg gcattgtaac atttgaattg
     8521 aaaggaggta ttgaaagcgg aagaaaattc ctgaatgcat taaaaatgat ctccatgacg
     8581 aataaccttg gagacagccg caccatagct tcgcacccgg cttcaaccac tcactcaaaa
     8641 ttaacggagc aggaaagaca ggctgtagga attacacccg ggttaataag gatctccgca
     8701 ggattggaga atgtggatga tatcctggct gatattttcc aggcgttgga taaatcctga
     8761 taacttattt actttttcgt ttcgcaagct gttgcaacgt agctctcatg tctttatgat
     8821 gtggtgcttc cagttctatc attgtaccat caacatcttt gaaaacaagc tggaaagcat
     8881 gaagggcaag gcgtccgagg atcggccttt cttccagttc atctttggaa agtttgtatt
     8941 tcttttttaa ggcagaaata agaattggtt ttccatcgcc ataaatattg tcagctacaa
     9001 tggggtggcc gatttctttc atatgcaccc ttatctggtg agttcgtcct gtgtgaatct
     9061 gaaattgcag tagtgaataa atgccaaaat cttccagcac ttcataatct gtcagggatt
     9121 ctttgccctt gcgatgaata atcatcgttc cgttaagggc aggatgttca actattgggg
     9181 aatcaatact gccttttttg ttagcgggag aaccaattac caagccggtg tagatttttt
     9241 ttgtttgccg gtcttcaaat tgtttgcaca ggtgtttatg tgcggcttca ttctttgcga
     9301 atacgatcag gccgctggta tcccggtcga tccggtgaac agtgaatata ttaccatatt
     9361 tttctttgag taatactttt aaagaaattt cttttccttc cctgtcagga atggataata
     9421 ggccagaggg tttattgaca gccacccaat tttctgtttc tgagatgatc caatcggtga
     9481 tcttcatttc aaacgtttaa atcaatgcaa tcagtggtta tttccctttt ccaaaatgtt
     9541 tcaggtctct cacttctttt tcacttagaa aacgccactt gcctctttct atatttttct
     9601 tggtaagccc ggcaaatatt acccggtcaa gattccttac atcgtagccc agcgattcaa
     9661 aaatacgtcg aacgatacgg ttacggccac tatggatctc gacaccaacc tgtgtcctgt
     9721 cttttgcatc tgcatacgcc agcacatcta cattggcagc gccatcttcg agggccacgc
     9781 cttttaatat ctgttcaaaa tcttttttat ccaacggctt attcaatgtg acgtggtaga
     9841 ctttttttac ttcattactg gggtgggtaa gtttttgtgc cagttctcca tcgttggtca
     9901 gcaataaaac gccggatgtg tttctgtcca ggcgaccaac aggatatact ctctccgggg
     9961 tagccctgcc tataatatcc agtactgttt ttcttccctg cgggtcatcg gttgtggtaa
    10021 tgtagtcttt tggtttgttc agtaaaatgt aaaccaggtt ctttgccagg aatatttttt
    10081 taccatttac tcttacttcg tctttggcag aaactttaaa tccgggttct gtaactaatt
    10141 cattatttac cttaaccagt ccttttcgta ccatttctgc ggcatcacgt cttgcagcca
    10201 caccagcatg tgcgatgaac ttatttaatg gcattgtacc cgttgtttcc ggtctgctgt
    10261 gcgatactgg cgaatgttgt cctcccgctg ttgattgccg gctctggatg gttacttttc
    10321 cccgggcttc tgcctttttc ttgtcaaaat actcttcccg ctctttcttt acttttcttt
    10381 tttcctgttt gaactgctct ttgacagctg cgttgctttt ctttttagcg aatttgccga
    10441 aattgttgga tgctttctta ttcatgtgta gtgtttgctg ttttacaaca gtagtgagcc
    10501 gcgaagatag ttattaatta caaagccctg tcattaactg acagggcttt gtatcttaaa
    10561 tattctcttt atattttcag attgtctctt tgtcttgtgg agcaggacct ttccggtgca
    10621 tacccggtcc gggtctgtgt ttttttcctt ctttcatttt tttcaactgc tcttctgtaa
    10681 ggattgattt catagattct tgctgttgtt tcatcagctc tttcatttct gctcttttct
    10741 tttcttcaga caatgattta ttttcccgga tggctttcat ctttgcgcct atttctttcc
    10801 tgctattatc catttttgca gattgttcgt cagttaagcc aagttgcgtt ttcattttat
    10861 cacttctttg cttcatcatt tcttcctgcc tggcttttcc ttctgctttc tttttttcca
    10921 attgggcttt ttgatcactg gtcaggatgc catccatttt agctttatgg tctttgcgga
    10981 gtgtctccat tttgcttttc cattctttca cggtgatgtt gtcattcttt ttcagctctt
    11041 ccatttgttt ttggaagctt tgtttttgta ctttaaactg ttctttctga gcctctgaca
    11101 ggttcaattg tttcatggcc atcgaacggt catgatgacc tttttgtctt tcatgcatca
    11161 ttggtctgcc gcctttcctt tccggaattt cctgggcgtt aactgtaaaa gctattgaag
    11221 ctaccaggac tgagaagatt atttttttca tggttataat tttgttttga gacgagggca
    11281 gctgtattaa accttcgcca cggtatgtta attaatgtga acgagtaaaa gaccagcctg
    11341 gccggcagga gtttaatgag atataggaaa attttcgtaa acggttaaaa ccggttatta
    11401 gtggctgtct ttattggcca attggccgca agctgcatca atatctttgc cacggctgcg
    11461 gcgcaggcgc acatttactc tgtgcttttc aaggtattgc atgaaagcct gcactttttc
    11521 ttcttctggt ttttcatacc tggcaaattc tatcggattg tattcaatta tatttatcag
    11581 gtcagcagga acctgccggt aaatttttat caactcatcc gcatcctgca gggagtcatt
    11641 aaaatttttg aaaagaatat attcaaaagt gatctcattg ccggtttgct tatagaaata
    11701 attcagcgca tctatgaggg tcttgatatt attggtatca ttaattggca tgatctcatg
    11761 ccgcttggca tcggtggcgg catgcaatga taacgctagc ttgaatttaa ctttatcatc
    11821 acctaactga cggattcctt tagccacacc ggcagtggaa acggtgattc tttttggact
    11881 cattgccagt cccgatggag aagtgatcct ctctatggcc cgtaaaacat tcttataatt
    11941 cagtaagggc tcacccatgc ccataaaaac aatattgctg agttttttac catgtgtggt
    12001 ttcacattgc ttattaatca gtactacctg gtcatatatc tcatcgaaat cgaggtttct
    12061 ttttctgtca atataaccgg tggcgcagaa tttacagctg agggaacatc cgatttgtga
    12121 agaaacacat gcagtgaatc tcgatttagt tggaatcagc acaccttcta ccaggtgacc
    12181 gtcccatgtc ttaaaacggg acttaatggt accgtcggat gaatattggg tcgcatcaat
    12241 tgtaagggcc ggtaaagtaa aagtatctcc cagcttttga cgcaggtcct tgctcaggtt
    12301 ggtcatatcg gcaaagctca tggcttgttt ttgccatagc cattcatgga cctgtttagc
    12361 cctgaatttc ttctcaccca tttcttcaaa atactgttcc agttccgcca gggtaagatg
    12421 ccgtatattt tttagtgctt ttttcatccg ggggcaaaga taaccactga tttacggatt
    12481 aaatgatttc acagatattt gctacaaata gtacgggatg aaatcagtgt atgtaattga
    12541 ctcggtaaga acccctgttg gtaaatatgg aggggtttta agtagtatcc gcccggatga
    12601 tttattggcg tatgtgataa aagcgttgct actaaggaat tctggcattg acttaaatgc
    12661 tattgaagat gtaattgccg gcgatgccaa ccaggcaggt gaagacaatc gtaatgtggc
    12721 aagaatggca gctttactgg ccgggttacc agtatctgtt ggcggcaata ctgttaaccg
    12781 attatgtgct tccggtttac aggcgatcat ggatgcttcc aggcagatca tgtgcggaga
    12841 tgcagaattg ataattgcct gcggagtcga aagtatgaca agggccccat ttgtgatgcc
    12901 caaaagcagt gacgcttaca gccggacaac tgaaatattt gatacaacta tgggctggcg
    12961 gttcatcaat aaaaaccttt ccgcactata ccatccgtat accatgggag aaactgctga
    13021 aaatatagcg aagcaatgga agatcagccg gcaggagcaa gatgagtttg ctttcgcatc
    13081 ccagcaaaaa tatgcggcag caaaagctgc agataaatgg gctgatgaaa tagcagcggt
    13141 tgagttgaat aacaagggat tgatcagttg ggtggtgaat gatgaacatc ctagagaaac
    13201 ttcattagat aaactggcag cattgaaacc agcttttata aaagacggaa cggttactgc
    13261 tggaaattct tccggaatta atgatggtgc agctgcaatg ttgctggcaa gcgaagaagc
    13321 agttagaaaa tacaatcttc agccgatggc aaaaattgtt tccatggcag tggcaggggt
    13381 tgatcctgct attatgggta ttggcccggt gcctgctact caaaaagcat tgcaaagggc
    13441 aggaatgaca atggcagatc ttgacctggt agaactgaat gaggcatttg cttctcaatc
    13501 cattgcctgt atccgtgacc tcgggctgga taatttaaaa gttaatgtga atggcggcgc
    13561 aatagcaatt ggtcatccgc ttggatgcag tggggtaaga atttcaacga cccttgttca
    13621 tgagatgagg aaacgaaatg ctaaatttgg attagcaacc atgtgtatag gagtaggaca
    13681 gggtgcagct gttatttacc agaatgtata aaaataacag ggtagttgtc ttttattgta
    13741 acttccattt tactattaca aaaattaaat ttatatgaaa cagttttttc ttactctttg
    13801 tgtacttctt gttttttcgg cagttaatgc acaaactgat tctttaaaac agtataccgg
    13861 taaatacatt tttccggaag gtagtcctgt aacagaaatt ggtgtagtgc tggaaaatgg
    13921 tttgttgact gccacttcgg ctatgggcaa ttccgaacta aagaatactg atactaaaga
    13981 tgtgtttgag gttgttgctt acggtggtat tgctactttc aagagaagtg cagagggcaa
    14041 ggtgaatggt gtacagatac aggtacagga tgtcaatatg gagggaacaa agacagaagg
    14101 gcttgcttat gctgaattaa aagttatgtt gcctgtatta cgaataagaa actgaaatca
    14161 ttttctgcat ggaaattgaa aggctgtact gtttgataca gccttcattt tttatcagaa
    14221 ctggtgttta agtaagtcag ctgttggtat agtttgctgt atacctgttg taaggttttt
    14281 aatattacag gtactttctt tcaactcttt ctcacccaga ataataacaa agggtatatt
    14341 cttcttctct gcgtatttaa actgcttgtc aaattttgct gattcatgat acaattcaca
    14401 acggacccct ttttgtctta attgctgcat caggaaaaaa gaagcagtac tttctgcctc
    14461 acccaggtta aaaaataaaa cctgtgttcc tatatgtaca tctggcggaa agaggtttag
    14521 ttcttccata acatcataga tcctgtccac gccaaaggat attccaaccc cgggaatatt
    14581 gggtacacca aaaagaccgg tcaggtcatc ataacgtccg ccgccgccaa tacttcccat
    14641 ttttacttca gccggggctt ttgcttcgaa gatgatccct gtataataat taaggcccct
    14701 ggcaagggtg gggtcgagtt taaggtttaa gtgttgaggt tgcaggttag caaggacgaa
    14761 gcttacttgt tctataccct tttttccgat ttcacttttg ccaattatct caagcaggct
    14821 attaagtatt tcatcatttg ttccgttgat gttcaggtat ttttcaataa tgtttacctg
    14881 ttcagtagtc aaacctcttt gtgttagctc ttcttttact ttgataatac caatcttatc
    14941 aagtttatca atggcgattg taatatccat catcttatca attccgccac aggtttttgc
    15001 aagggctgtt aagattttgc ggtggttgat tcgtagttca tatccatcca gctttaatct
    15061 gctgaacact tcatgataga tagaagctaa ttctgcttca tttaataaag cattactccc
    15121 taccacatcg gcatcacatt ggtaaaattc cctgtacctc cctttttggg gtctgtctgc
    15181 tctccaaacc ggctgaacct ggtagcgttt aaaggggaat gtcaaatggc tatgattcat
    15241 tgccacataa cgggcaaagg gaatggtgag gtcatatttt aatgctcttt ctgtaatccc
    15301 ttttacattc ttaccctgta aaaccttttc aaaatcttct cttatttgtt tttccttttc
    15361 cgggttatca agtccgttat taaggatctt aaaaatcaat ttgtcgcctt cctctccgta
    15421 tttacccatt aatgtttcaa tattttccat tgccggagtt tccagtggct gaaaaccata
    15481 caactcaaac acagatttta tgatgtttaa aatataactc cttttccgga caacatccgg
    15541 tccaaaatcc cttgttccct gaggtaatga tggtttagcc attataacgt tttacttgtt
    15601 attcaaatct gccggttgct gattaactat ggcatatttt tcaatgatat tatcacacac
    15661 ttcatcaatc attttgtaca cttcgtgata gcctttttcc ggtccgtagt aagggtcagg
    15721 tacatccaaa tttttttcag gatacaattc attcatcagc aggtcagttt ttgctgcatc
    15781 aaatttattg cgggctattc tctttatttc atccattaca tcttctgcca gtgcatagat
    15841 tttatcatac tggtcaaaat cttctgcctt aaaacgcctg gccctttggg aacttatatc
    15901 aatgccattc tgttttgcca ctttttgcga aagcggatgt ggtgcttcgc ctatatgata
    15961 gctgtttgtt ccggcacttt caacacacca ggttagtcct gccttaaaag ccttatcctg
    16021 caggacacct tcggctatcg ggctgcggca gatattcccc aaacagacca ttaaaatttt
    16081 catacaggca aagataggaa tgctggtgca tggttcattg atcatggaaa tgtcactttt
    16141 tgaactatga acaataaggt tatggattat tagtgtgctg gcatctaata atcaaacaca
    16201 cagccatgac aaacaaagaa attcttcaag ccagtttgct cgacattgta ttcgacaaac
    16261 gtaacaaagc atatggtgca tatgcactcc gtaaagggta taatgccagg ctacttactg
    16321 cactagctgc aggtatgtca gttattcttc tctttatttt cattaatggg atgcagggga
    16381 gtaaaaattc ttcgtttatt accagtaata aaaatgaagg aattgttata aagcaaattg
    16441 aattgccgaa agataaaccc gccgagccgg agaagccaaa agaggtaata aagcaaaagc
    16501 cagtggagaa aattgctaca gcaaaatata tcagtaatat aaaaattgaa aaggatgtaa
    16561 aatccgctat gacggcaata ggcgatctgg atggcaaggc aattgacagt aaagagggtg
    16621 atgggaaaat agatgatggt actgaaattg taaacgttga tcctgttgcc ccggttggaa
    16681 acggagccat acctgctgaa cccaaacagc cagattttgt gatccaggaa agaaaccctg
    16741 agtttcccgg gggggctgaa gcattgaaga gatttctgtc gaaaaacctg gatacacccg
    16801 gtgaactgga agcgggagaa atgaagaccg ttaaaatcag gtttaaagtg gataaagatg
    16861 gttctgttaa tacctttgaa atagtgacga gtgctggtaa tgattttgac catgaagtag
    16921 ttagggtatg taagaagatg ccaaaatggg tacctgcaat acagaacggg gtaaatgttc
    16981 cggtaaacta tgtactgcct gtcatatttg tgggttcaga ataataaaat ttctataatt
    17041 tgctaggtag caattgtccc tggtaagttt ttctgccggg gacgattttt cggcaaaaga
    17101 atttggattt taatgacaaa ttagttgaca atgttagaag aatatgaaaa gaataagcgg
    17161 aagcagattt caggaatgaa gtcgatcatg gactatggaa tgggtctgct tattttactt
    17221 ttggggctat tttttttatt cagaatgaaa ttgggtaata tacctatcaa cgacagattg
    17281 ggtcagccag attctataga aaaggtattt ggtgtttttt gcctcattta tggtgcctgg
    17341 cgtatttaca gagggtatca gaaaaaatat ttccgatgat ttttcgccgt actatgaaat
    17401 tgaagaaaag gctttttccg gttttcatgt tattgttatt attaacagca tgtaaatcat
    17461 atgaagaaca ggaaaatgaa ttaccggaca gccgtaaccg gggaactata agggtaagtg
    17521 ctgatgaaac ttttaaaccg gtaat
//
LOCUS       DAORTI010000069        17390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2945_length_17390_cov_7.034324, whole genome shotgun sequence.
ACCESSION   DAORTI010000069 DAORTI010000000
VERSION     DAORTI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_17390_cov_7.034324"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..542
                     /locus_tag="PLO70_09840"
     CDS             144..542
                     /locus_tag="PLO70_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74810.1"
                     /translation="MAKLEAGSTLQRLRNRYRLVVMNDDTYEEVVTFKLSRLSVYVML
                     STIFVVLVGLTVALIVFTPLKYYIPGASTDYKSVVELKRLQYRVDSVERQDAYKTQYI
                     LGLKKALGSDGTAQLDTTTIVLPKIDISND"
     gene            581..967
                     /locus_tag="PLO70_09845"
     CDS             581..967
                     /locus_tag="PLO70_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQZ74811.1"
                     /translation="MQQPVTNGNGGATLISSGTILKGDISSNNDLRIDGTVIGNVNSS
                     AKIIVGTSGVVEGDIAGNQADITGKVSGNIRAKELLQLRGDCLVTGNLYAGKLQVEPS
                     ATFNGQCHMGANIVEMSTNEQAAAIQ"
     gene            942..1169
                     /locus_tag="PLO70_09850"
     CDS             942..1169
                     /locus_tag="PLO70_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQZ74812.1"
                     /translation="MNKRQQSNNNNSDILRYASLGTQILVAIGLAVFAGIKADKWLHT
                     FPLLACVLPLLVLSAIFYKVFRETSRPKKDE"
     gene            1162..1563
                     /locus_tag="PLO70_09855"
     CDS             1162..1563
                     /locus_tag="PLO70_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74813.1"
                     /translation="MNKDLLRLLRPLLIIFILINAFAITGKSWLEKNSVDQSVLIAGN
                     LLLFLVSLTAFLITYRSLRSSNPQAFVRAMYGSFMIKFFLIAIVAFLYIMIARKNVNK
                     PGLIACGILYIVYTVLETGALMKLLKQRKNA"
     gene            1556..2182
                     /locus_tag="PLO70_09860"
     CDS             1556..2182
                     /locus_tag="PLO70_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HQZ74814.1"
                     /translation="MPKKEVPVDHLQQFLPPGTGAAVINYLHQYKVHLTIAAERTSIL
                     GDYRHRTHHANHRISVNGNLNTYSFLITLLHEIAHLLTFEQYGNKVQAHGKEWKMIFG
                     QLLQQFVRHKIFPADIEKELLQSLKNPAASSCAEEGLLRVLRNYDAKKSNHHLVEEIA
                     PGTLFRIKDGRVFRKGEQLRKRFKCIEIKTGKMYLFSPVYEIELLPAD"
     gene            2264..2812
                     /locus_tag="PLO70_09865"
     CDS             2264..2812
                     /locus_tag="PLO70_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ74815.1"
                     /translation="MKKIILGIAALTGLMVATAQTNQGDWMVGGNFRLNTSDNDSEIS
                     FTPSAGAFIVDNLALGGNFSLGYSKSGNNKYTTFGIGPFARYYFTTESQAVRPIIHGS
                     FNYLSTKQKIGNVSSTNNGTNFFIGGGAAMFISKNVSIDALLGYDRTKYKNFSGSGGF
                     AFNVGFQVYLLKDQVEKVTGKK"
     gene            complement(2862..3344)
                     /locus_tag="PLO70_09870"
     CDS             complement(2862..3344)
                     /locus_tag="PLO70_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74816.1"
                     /translation="MNTLIRPIQPPDNPIIAKIIRDTLTEFGANHPGTVYYDPTTDAL
                     FELFQKERSAYNIAVTDGRIVGGGGIYPTDGLPADTCELVKMYLLPEARGTGLGSQLI
                     TKCLEQAKNNGFSKVYLETMNELKAALKVYARMGFTYLDGPMGNSGHFGCPLWMIKEL
                     "
     gene            complement(3541..4299)
                     /gene="bla"
                     /locus_tag="PLO70_09875"
     CDS             complement(3541..4299)
                     /gene="bla"
                     /locus_tag="PLO70_09875"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_079473658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HQZ74817.1"
                     /translation="MNPLIKTLVIVITSLTFSNCSSQQQDNFKPKEVYKSNDLIIIQL
                     AENAFQHTSFKQTNDFGNVPCNGLIVRNSNETIVFDTPTNDKTSEELIKWIEETIHCR
                     INAIIPTHFHDDCLGGLHAFNKNNIPSYANFKTIELAKENKLAVPKTGFSDSLILKVG
                     AETIIAKFFGEGHTKDNIVGYYPKENILFGGCLLKELEASKGYLGDANTAAWSATVEK
                     IKKQYPDIKIVVPGHGEHGNKSLLDYTINLFRAQ"
     gene            complement(4557..5768)
                     /gene="sucC"
                     /locus_tag="PLO70_09880"
     CDS             complement(4557..5768)
                     /gene="sucC"
                     /locus_tag="PLO70_09880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQZ74818.1"
                     /translation="MNLHEYQAKELLKKYNVPVQDGFACSTVHEAEEAYRQIKTQTGS
                     KFAVIKAQIHAGGRGKGTIKENGINGVKVAKSLEDVQEFSSKILGGTLVTLQTGPAGK
                     VVNKIFVAADMYYDGHSERKEFYLSILLDRTKGQNVIMYSTEGGMNIEEVAHNTPEKI
                     FKEWVHPAGGLQGFQARKIAFNLGLSGEAFKNCVKFVTNLYNAYVGLDCSMLEINPLF
                     KAADDKILAVDCKMNLDDNALVRHADLSSLRDVTEEDPTEVEAGQYNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFKIILKDPAVKAILINI
                     FGGIVRCDRVAQGVIDAYKNLGNINIPIIVRLQGTNAVEAKKLIDESGLKVQSAILLS
                     EAAELVKKAVA"
     gene            5992..8718
                     /locus_tag="PLO70_09885"
     CDS             5992..8718
                     /locus_tag="PLO70_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQZ74819.1"
                     /translation="MKQVISFFYLVLIVFSVNAQITLSGTNYSQNFDGVGSGLPSGFR
                     VSSSATAATLGTAATFNSAIDANSPWNSTFSGWKNVASADGLISSSNAATQAASTDRV
                     VGIRQSSSLGDPGAALEMQLANTTGFSDFSMSFKLQSLDATSARITTWRVDYGFGASP
                     SSFNTIATSPASITTGGGTFSNNTVTVDFGTALDNINQNVWIRIVTISATTLSGSRAT
                     TGFDDISLDFSIGSGCTPPATQASAVSFSSVTSSSFDINWTAGSGSNSLVVIKAGSAI
                     NGTPSNGIAYTANAVYGAGQIIASGEYVVYNGTGNTVTVTGLSTSIIYHVAVFSFDNT
                     GSCYNAVSPATANVITACGQPAIQVSTISTNASLTSASLNWSGGSGTNALVKINTSNS
                     FTAPLDGVTYSTNTLYSGGEQVIYNGTGTAAYVTGLLPGTNYYVTIYTYNSCGSSPDY
                     LVTGNPVQPFTTLLNNNIPANYYNTASGLSCAPLKTELRNIINTGLTHQSYGDLWTIY
                     QTADVQPSELASGNVIWDIYSDDPAGPDPYEFTPVSDQCGGYSGEGDCYNREHSFPQS
                     WFVGGTSAGPGTDYHHIFPTDGHVNGQRSNYPYGSVGTATYTSFNGSKLGSSNWTGVT
                     GTVFEPINEYKGDVARAFLYMVTCYQNSMPGWESLDVSGDVAMDGTLWPSVEPSYLQL
                     MISWHNSDPVSQKEIDRNNAGYSFQGNRNPFVDHPEYVEMVWSGLCYALPVNIISFTG
                     KSQGSFVELNWTIANAENFGQFVVERSVNGGLSYEEIGEVEYRQNFSAYSYTDNVSLL
                     KGEVLYRLKMVNLDGSSRYSKVIRLSITGINRISLYPNPATDQLYVAFYQPTRADYTV
                     WIADMSGRKIFTKLLPAGQLNYQLPVSNLPAGPYILSLHSPEQVIFQKFIRK"
     gene            8779..9597
                     /locus_tag="PLO70_09890"
     CDS             8779..9597
                     /locus_tag="PLO70_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74820.1"
                     /translation="MYRVPLIFLILWLLTACNHTKQLQAPTRQSNLTGTDFYKQASAM
                     KWESRDSFVLKEVFAGNLPSFLKKFAPVHVSIRDSVSGKTIRAVYFVSPDYLCIGTDN
                     DWARINITPLAAQKIADSFNCFLPTRKMADDIYKAAKVKLEPVPMYAFRDSTPTMWHH
                     HLVIEGQRKGREGLIAGIKKDVVISGRISRDKRPDRVAIYGWHMLNGKPIQPLYTGHI
                     NWWVDYSQGIRLVYRQLKVEGKWMDYTVVLKDPVLQKLLCDEEFCDFYRYDYYP"
     gene            9774..11765
                     /locus_tag="PLO70_09895"
     CDS             9774..11765
                     /locus_tag="PLO70_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74821.1"
                     /translation="MLITPVLSSAQDPMIQQMGNRIKQVGGSMRSGSGSTDSLQRRNK
                     MEDSITISFRYLDSTKSYKLDSTVTDFTRRFPLPATYINLGNTGNAARSLLFSPNFNA
                     GFDPGFHAFDVYKWKMSDVRFFNTTRPYSELNYMLGSRVEQMISLLHTQNIKPNWNFL
                     FQYRLINSPGFFQNQKTNHNNYLLSSKYQSKNLRYHNYFVLLGNKLQSAENGGIIDTS
                     DILNDPIYKERFNINTRIGGVSQFSSNFFSNKINTGNKYSEFTLLLRQQYDFGKKDSI
                     VTDSTVLPLFFPRVRFEHTLQLDQNKYIYQDNVADSVYYKTYYDTSLRYPKDTLLFRE
                     RWQVLTNDFSIYQFPDAKNLQQFIKLGVLLQNISGELASGKENFLNTAGHAEYRNKTR
                     NQQWDIEANGKLYFTGFNAGDYQAHISLQRMLGKKLGYLQLGFENISRTPSFIFDSRS
                     SFYLLKTVKDFKKENSTHLFASYFLPSFKLRLTGHYYLATNYTYFTNYYEAQQESVLF
                     NVFRLGLEKTIKFGKRWNWHTDIYFQQVVGDAPVNVPAIYTRNRIAYEGNLGFKNLDI
                     AMGVELRYRAAYKADNYSPALGQFFYQDSITIRNPLPDISGYVHFRIRPFTAFIRFEN
                     LNTGQNLEGFSFTRNNLVAPDYAMPGLQFRIGIFWKFVN"
     gene            11836..12228
                     /locus_tag="PLO70_09900"
     CDS             11836..12228
                     /locus_tag="PLO70_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74822.1"
                     /translation="MKKVIASISVLCYFVVTCGVVVNFHYCMDKLASVHFFGDKSDKC
                     GQCGMVVHESDGCCHDEVKVIKLEQDQNKTAAADYSIPSLKELAITPSEFIAASFYNI
                     MEECHFYNHSPPLLSAQDTYLQINVFRI"
     gene            12328..12957
                     /locus_tag="PLO70_09905"
     CDS             12328..12957
                     /locus_tag="PLO70_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQZ74823.1"
                     /translation="MKTLRIFSIVALLMAISSLSFAQTKTETFNVSGNCGMCKTKIEK
                     AAKDAGASAASWDVDAKQLTVTYKSSTTNAAKIQQKVAEVGYDNAGFKATAEAYNKLH
                     GCCKYERETAAASENHSCSGKCDMKDGKCADMTDGKKMDCAKDGKCTKHVHEGQTGEK
                     MDCCKDGKCSMPGHDGKDCCKKDDAKMDCCKDGKCSMPGHDGKDCCKKS"
     gene            12983..13528
                     /locus_tag="PLO70_09910"
     CDS             12983..13528
                     /locus_tag="PLO70_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQZ74824.1"
                     /translation="MKKVLLIITMAFFALQSYAQFSKARLQATGLTCAMCSNAINKAL
                     EKISFVESVKSDIKNSAFNIEFKQGADVNMDALKDAVEDAGFFVGGFQVTGVFTEMKI
                     DPDKYAKIGNYNFHFLNVSSRILNGEITLKVLDKSYLTAKEFKKLSSSVKAPCLQTGK
                     ADGTHVKDGVTAGERVYHVTI"
     gene            13592..14083
                     /locus_tag="PLO70_09915"
     CDS             13592..14083
                     /locus_tag="PLO70_09915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ74825.1"
                     /translation="MKIFRQYFIILFSLFFSVDAIAQVKSGYKAPEVALPSTTGDTIL
                     LSSLKGKVVLLDFWASWCKPCRASNKGLVKLYPKFKDKGFEILGVSLDEDKADWKKAI
                     KQDKINWLQVNDPGGWQSPTVLQWRINAIPTSYLINRNGELIAMDLEGKELEKALKEI
                     LGN"
     gene            14097..14924
                     /locus_tag="PLO70_09920"
     CDS             14097..14924
                     /locus_tag="PLO70_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74826.1"
                     /translation="MLRFLFSFLLLFALRLSGQELYVFSEPASNVPAHSMSIKLKEHF
                     VTSDRIYERFAGRFMPQIMFGVSKKLMIRAGASIANMHTDNSRFESFSFYTKYRFLSK
                     DELHKHFRMAVYAEASRTNAPFHYDEISMMGDKSGVELGIITTQLWNKFALSGTVSHT
                     QVLDKSRNDKVLYIPERNYQSVNFSLSGGYLLLPREYTDYKQTNLNLYLEILGQQTFE
                     NPSYYVDMAPAIQLIFNSNTKLNMGYRFQLGSDMQRMAANSWLLSIERTFLNALKKR"
     gene            14952..>17390
                     /locus_tag="PLO70_09925"
     CDS             14952..>17390
                     /locus_tag="PLO70_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ74827.1"
                     /translation="MKILKRDIFFAAFVIIQLAGTDIYAQESAGNSTDTVVVINVDGA
                     CEMCKERIETVAKDKKGVRDAVWDIESKKLKLVYNPLLITVEKIQCSIADAGHDNDYE
                     KAKDKVYEKLPACCRYRGINHPKEKPGENNLNNNAANPVVTQVVKGVVLEENKQGSFV
                     PLMGASVLWLGTNTGVVTDTAGVFIIKNEKGYGRLVISYTGYEPDTLVVTNAEELKII
                     LASGRQLNEVTVSAKQRSTYLSSLNPIRTQVMTERELFKAACCNLSESFETNPSVDVS
                     YNDAVTGSKQIQLLGLSGNYTQLTVENLPGPRGLATPFGLNFIPGPWVESIQLNKGVG
                     SVVNGYESIAGQINIELKKPATAEKLYLNAYINDMTKTDLNLVLTEKFGKNWSTALLL
                     HDNFQTKKNVDYNDDGFRDMTTGNLFTAVNRWNYNGGKGFESQLGFKILNDDLTGGQT
                     SFDPDKDKFTTRSYGLGINTRRYEVFAKAGYVFPGKKYKSIGLQLSAFDHKQDSYFGM
                     TSYKGRQKNFYANLIYQSIIGTTAHKFRTGMSFLSDNYDELFNSIKYQRQETVPGAFF
                     EYTFTPVEKFNVVAGMRADHNSLFGWFATPRLHIRYEPVTGTTIRLSAGRGQRTANIF
                     AENNSVFVSSRQMNILTASAGKAYGLDPEIAWNKGLSVDQKFKLFKRNANLGLDFFRN
                     DFQQQVIVDLEDARQVKFYNLDGKSYSNSFQAEMNMEPARKLEVRLAYRFFDVKATYG
                     NQLLQKPFTAKHRAFTNLAYEISSWKIDYTFNYNGSKRITNTTANPAIYQRQVNSDSY
                     ILMNAQVTKTVGKKN"
BASE COUNT         5085 a         3274 c         3822 g         5209 t
ORIGIN      
        1 tttccgtcgc aatctaaagt cttattaact gaatttcaaa taatttagta taaaactaag
       61 gcaatttttt tgacctgttt taccagcagt ttaggtgtaa aacccggccg catttcctat
      121 ctttagagac caaaaatcct tttatggcca aattagaagc cggttcaacc ctgcaacggc
      181 tgagaaaccg ctaccgccta gtggtaatga acgatgatac gtatgaagag gtggtgacat
      241 ttaaactctc caggcttagt gtatatgtca tgttgagcac aatatttgta gtattggtag
      301 gtcttacagt agccctcatc gttttcacac ctttgaagta ctatattccc ggtgcaagca
      361 ctgactataa atcagttgtg gagctgaaaa ggcttcagta cagggtagat tcagtagaaa
      421 ggcaagatgc ttataagacc cagtatatcc tgggtttaaa aaaggccctg ggtagtgatg
      481 gcaccgctca actggatacg accactatcg tgctgcctaa aattgatatt tctaatgact
      541 aatgaataaa accatgttca acactaaaaa caaaaccgag atgcaacaac ctgtaacgaa
      601 tggtaacggc ggagcaactt tgataagctc cggcactata ctaaaaggag atatcagcag
      661 taataatgac ctgcggattg atggaaccgt cattggtaat gttaatagct ctgccaaaat
      721 aatcgttggt accagcgggg tggtggaagg agatatcgcc ggaaaccagg cagatattac
      781 cggaaaagta tctggcaata tcagggcgaa ggaactgtta caacttcgcg gagattgttt
      841 ggtaacgggt aacctgtatg ccggtaaatt acaggttgag ccttcggcta ccttcaatgg
      901 tcaatgccat atgggagcta atattgtaga gatgagcact aatgaacaag cggcagcaat
      961 ccaataataa taacagcgat atactgcgtt atgccagcct tggcacacag atactggtgg
     1021 cgattggcct ggctgtgttt gcgggaatca aagcagacaa atggctgcat actttcccat
     1081 tgctggcctg tgtattacct ttgctcgtcc tgtcggcaat tttttataaa gtattccggg
     1141 aaacaagcag gccaaagaaa gatgaataaa gatttactgc gattactacg tccgttattg
     1201 atcattttta ttttaattaa tgcttttgct attaccggta aaagctggct ggaaaaaaat
     1261 agtgttgatc aatctgtatt gattgccgga aaccttttgt tgttcctggt aagccttacc
     1321 gcttttttga taacctatag gtcgttgcgt tcttccaacc cgcaggcttt tgtaagggcg
     1381 atgtatggta gtttcatgat aaaatttttc ctgatcgcta ttgtggcttt cctttatatt
     1441 atgattgcca ggaagaatgt aaataagccc gggttaattg cctgtggtat tttatatatc
     1501 gtgtataccg ttcttgaaac cggtgccctg atgaaattgc tgaagcaaag aaagaatgcc
     1561 taagaaagaa gtacctgttg accatctgca gcaattcctt ccacccggta caggggcggc
     1621 agtgatcaat tacctgcatc agtataaagt acacctgacc attgcagcag aacgtaccag
     1681 tattttgggt gactaccgtc atcggacaca tcatgccaat caccggatca gtgtaaatgg
     1741 caacctgaat acctattcat ttcttataac attgttacat gaaatagccc acctgctcac
     1801 ttttgaacaa tacggaaata aagtacaggc tcatggcaaa gaatggaaaa tgatatttgg
     1861 tcaattgttg cagcaatttg tccggcataa gatctttccg gcagatattg aaaaagagtt
     1921 attgcaatca ctgaagaacc ctgctgcaag cagttgtgcg gaagaaggat tattgagggt
     1981 tttaaggaat tacgatgcaa aaaagagcaa tcaccatctg gtggaggaga tagcaccggg
     2041 cactttattc cgcattaagg atggacgggt attcagaaaa ggggagcaac tgcgaaagcg
     2101 gtttaagtgc atagaaatta aaacgggtaa aatgtatctt ttcagcccgg tttatgaaat
     2161 agaattattg ccagccgact gacagctgtt tagatttttt ctacaaaatc attcttcctc
     2221 ttatttttca ttttctacct ttgcggcaca aaaatcttta agtatgaaaa aaattatttt
     2281 aggaattgca gcactgacag gattgatggt agcaactgca cagacaaacc agggcgactg
     2341 gatggtaggt ggtaattttc gcctgaatac atctgacaac gattcagaaa tttcttttac
     2401 gccgagtgcc ggtgctttta ttgttgataa tcttgctttg ggaggcaatt tttctttggg
     2461 gtattccaag tcgggaaata ataagtatac cacttttggt atcggaccat ttgcccgtta
     2521 ttattttact acagagtcgc aggcagttcg tccgatcatt cacggtagtt tcaattacct
     2581 gagcactaaa caaaagatcg gtaacgtatc gtctaccaat aatggtacta atttctttat
     2641 cgggggtggt gctgccatgt ttatcagtaa aaatgtatcc attgatgcat tgctgggtta
     2701 tgatcgtacc aagtacaaaa atttcagtgg tagtggcggg tttgctttta atgtgggttt
     2761 ccaggtgtac ctgttgaaag accaggtaga aaaagtaaca ggaaagaaat gatattattg
     2821 acgtaaaaat caaaagaggc ctgtaacggg cctcttttga tttacagttc ttttatcatc
     2881 cataaagggc aaccaaaatg cccgctattg cccataggcc cgtcgaggta ggtaaatccc
     2941 atccttgcat aaactttcaa tgccgccttc agttcattca ttgtttcaag ataaactttg
     3001 ctaaatccat tatttttcgc ttgctcaagg catttggtga tcaattgaga acctaagccg
     3061 gtacctctag cttctggcaa tagatacatt tttaccagtt cgcaggtgtc ggccggtaaa
     3121 ccgtcagtag ggtagatgcc accaccacca acgatcctgc catctgtcac tgcaatattg
     3181 taagccgatc tttctttttg aaataattcg aataaggcat ctgtagttgg gtcataataa
     3241 acagtaccgg gatgattggc tccaaactcc gttaatgtat cccggatgat ttttgcgata
     3301 atgggattgt ctggtggttg tatggggcgg atgagggtgt tcatgttgta aagataaatc
     3361 ttcgccgggt agggtgatta cttgaaccat tatactgaat caggttcagt acaattttgt
     3421 gaagattatt tggattgtga ggatttctta ttccggccat gagccggaat ttttttgtga
     3481 atagccggag gctatagcat agggccgtga agctgcatac cctgcagatc tgttgtttat
     3541 ttattgtgcc ctgaataaat taatggtgta atcaagcaat gatttgtttc catgttctcc
     3601 gtggccgggt accacgattt ttatatccgg gtactgcttt ttgatttttt caacagtggc
     3661 tgaccaggct gctgtattgg cgtcgcctaa ataacctttg cttgcttcta actcttttag
     3721 taaacatccg ccaaatagta tattttcctt tgggtagtaa cctacaatat tgtcttttgt
     3781 atgtccttcc ccgaagaatt tggcaatgat cgtttcagcc ccgactttta aaatgagtga
     3841 atcactgaaa ccggttttgg gtacagccag cttgttttct tttgccagtt cgatggtttt
     3901 gaagttggca taggagggga tattgttctt gttaaatgca tggaggcctc ccaggcaatc
     3961 gtcgtggaaa tgtgtgggaa taatggcgtt tatcctgcaa tgaatcgttt cttctatcca
     4021 tttgatcaat tcttctgaag ttttgtcgtt ggtgggggtg tcgaaaacaa tggtttcgtt
     4081 gctgtttctt acgatgaggc cgttgcaggg aacatttcca aaatcattgg tttgtttaaa
     4141 ggaagtgtgc tgaaaagcat tttcggcaag ctggataatg atcaggtcat tggatttgta
     4201 cacttctttg ggtttgaagt tgtcctgttg ttgggaactg cagttggaaa atgtcaggga
     4261 ggttataact atcactagtg tttttatgag gggattcatt tttttggatt tctgttgact
     4321 aatgtttcct ggtaatgtat agtttaaatt taatcaagtt ttaattagct cagtagaatt
     4381 accggacgat gaagtgagtg acacaaccac aaaacattga agcactgaag ctggttacct
     4441 aataaaaatc ccggccaatg accgggattg aatattattt gattcaccca tcgacccttc
     4501 aattcctcaa ggtaaactga gctcccgcca aggcggtcag gcagggtgac agtgggttat
     4561 gccaccgctt tcttcaccag ttctgctgct tcgctcaata agatagctga ctgcactttc
     4621 aacccgcttt cgtcaatcag tttttttgct tccactgcat tggtaccctg taagcgtaca
     4681 atgatcggga tattgatatt gccaagattt ttgtaagcgt cgatcactcc ttgtgccacc
     4741 cggtcgcaac gaacgatccc accaaagatg ttgatcagga tcgcttttac agcagggtct
     4801 tttaggatga tcttgaaacc agcttctaca gtttgtgcat ttgcagtacc tcctacatca
     4861 agaaaattgg cgggttctcc accactaagc ttgatcatat ccatggtggc cattgccaaa
     4921 ccggcaccat ttaccatgca gccaacatta ccatccagtt ttacaaagtt caggttgtat
     4981 tgtcccgctt ctacttcagt cggatcctct tcggtaacat ctcttaaaga ggaaagatca
     5041 gcatgacgaa ccagtgcatt atcatccagg ttcatcttac aatcaacggc caggatttta
     5101 tcatcggctg ctttaaatag cggattgatc tccagcatgg aacaatctaa tcccacataa
     5161 gcattgtata gattggtcac aaacttcaca cagttcttaa aagcttcacc gctcaatccc
     5221 agattaaaag caatctttct tgcctggaat ccctgtaatc cgccggcagg atgtacccat
     5281 tctttgaata ttttttcagg ggtattgtgc gctacttctt cgatgttcat tcctccttca
     5341 gtactgtaca tgatcacatt ctggcctttg gtgcgatcca gcaggataga tagataaaac
     5401 tctttgcgtt cactatgacc atcataatac atatcagccg ctacaaatat cttgttcacc
     5461 actttacctg ccgggccggt ttgcagggtt accagtgttc ctcccagtat tttgcttgaa
     5521 aactcctgca catcttccag tgatttagcc acttttactc cgttaattcc attttctttg
     5581 attgtgcctt taccccggcc accagcatgt atctgtgctt ttataacggc aaatttactg
     5641 cctgtttgag ttttgatctg gcggtaagct tcttccgctt catgtacggt gctgcaggca
     5701 aatccatcct gcacaggaac attgtatttt tttaataatt ccttggcctg gtattcgtgg
     5761 agattcataa tggaaaaatt tgccgcgaag atacttattc agagtcaagc aaaaaggggt
     5821 aaagggctgt ttttgggaaa atcgtgcctg gttttttacc gggatgagca gcaggctcat
     5881 tttatcaacc agatattgac agcctgcagc ggaagaaata tatcagcttt tccagttact
     5941 taaacgctta gatttgctcc cttttataat tattgataat atacaatgtt gatgaagcag
     6001 gtaatttcat ttttctatct tgttttaatt gttttttcag ttaatgcaca gatcacttta
     6061 agcgggacaa attactcaca gaattttgac ggagttgggt cgggtttgcc ttcaggtttt
     6121 agagtgtctt catcggcaac tgctgccacg ctggggactg ctgcaacttt taattcagcc
     6181 attgatgcta attcaccctg gaacagtaca ttttccgggt ggaaaaatgt tgcttctgca
     6241 gatggcctta tcagttcatc caatgcagca acgcaagctg caagcactga ccgtgttgtt
     6301 ggcatcaggc aaagcagctc cttaggtgat ccgggtgccg cattggaaat gcaattagcc
     6361 aatacaaccg ggttttctga tttttcaatg agttttaagt tacagtcact tgatgcaaca
     6421 tctgcaagga taacaacctg gcgggttgat tatggatttg gtgcttcacc atcaagcttt
     6481 aatactattg ctacttctcc ggcttctatt acaacgggtg gaggcacatt ttcgaataac
     6541 acggtgacgg ttgatttcgg tactgccctt gacaatatca accagaatgt atggatcagg
     6601 attgtaacta tatctgccac caccttatcc gggagtcgtg ctactacagg ttttgatgac
     6661 atttctcttg atttttccat tggttcaggg tgtactccac ctgccacaca ggcatcagca
     6721 gtctcctttt catcagtgac aagcagttct tttgatataa actggacagc aggcagcggc
     6781 tccaactcat tagtagtgat aaaagccggc agcgctataa atggcacccc ttcaaatggt
     6841 atagcatata cagccaatgc cgtatatgga gccggacaaa tcattgccag cggcgaatat
     6901 gttgtttaca atggcacagg taatactgtt actgtaacag gcctttcaac aagtattata
     6961 tatcatgtag ctgttttcag ttttgataat acagggtcct gttataatgc tgtgagccct
     7021 gccaccgcca atgtaatcac tgcctgcggg cagccggcaa ttcaggtaag tactatctcc
     7081 accaatgctt cgctgacctc tgcttcttta aactggagtg gaggaagtgg aactaacgca
     7141 ctggtaaaaa taaatacatc taacagtttc actgctcctt tggatggagt aacatacagt
     7201 acgaatactt tgtatagtgg gggagaacag gttatatata atggaacagg cactgcagct
     7261 tatgttacag ggttgttgcc agggactaat tattatgtaa caatctatac ttataattca
     7321 tgcggttcat cacctgatta cctggtgaca ggcaatcctg tgcagccttt taccacatta
     7381 cttaataaca atataccggc taattattat aatacagctt caggccttag ttgtgcacca
     7441 ttgaagactg aactccggaa tattatcaat acaggactta cgcatcaatc atacggtgac
     7501 ttgtggacca tataccagac tgcagatgta caacccagtg aactggccag tggaaatgtg
     7561 atctgggata tttattcgga tgatccggca ggtcctgatc cgtatgagtt tacacctgtc
     7621 tccgatcaat gcggtggtta tagcggggaa ggagattgct ataaccggga gcattctttt
     7681 ccgcaaagtt ggtttgttgg aggtaccagt gcaggtcctg gtactgatta tcaccatatt
     7741 tttcctacag acgggcatgt gaatggtcaa cgttctaatt atccctatgg cagtgttgga
     7801 acagctacat atacttcttt caatggcagt aaacttggta gcagtaactg gaccggagta
     7861 acaggaacag tctttgagcc tattaatgaa tacaagggtg atgtggcccg tgcattctta
     7921 tatatggtaa cctgttatca aaacagcatg ccgggatggg aatcattgga tgtgagcgga
     7981 gatgtggcga tggatggcac attatggcct tcagtagaac catcttattt gcaattaatg
     8041 ataagctggc acaacagtga cccggtaagt cagaaagaaa ttgacaggaa taatgcaggt
     8101 tattcattcc aggggaaccg gaatccattt gtggatcacc cggagtatgt agaaatggtt
     8161 tggtctggtc tatgttatgc attgcctgtt aatattattt cttttaccgg aaagtcacag
     8221 ggtagttttg tagaacttaa ttggactatt gccaatgcag aaaactttgg acagtttgtg
     8281 gtagagagaa gtgtgaacgg aggattgtca tatgaagaaa taggtgaggt ggaatacagg
     8341 caaaattttt ctgcttattc ttatacggac aatgtttctt tattaaaggg cgaagtttta
     8401 tatcgactta aaatggtcaa ccttgatggc agttcaagat atagcaaagt gatcaggctg
     8461 tctattaccg gtatcaatag gatcagcttg tatcccaatc ccgctacaga ccagctatat
     8521 gttgctttct atcagccaac cagggcagat tacactgttt ggatagctga tatgtcggga
     8581 agaaaaatat ttacaaagct gctgccggcc gggcaattaa attaccagtt accagtcagt
     8641 aacctgcctg caggaccata tatattgagc ttgcattccc cggaacaggt aatttttcag
     8701 aaatttataa ggaaataatt ttacagacga aaggttgaaa agctgtcttt tataagacag
     8761 ctttttttat cttgagctat gtaccgggtt cccttaatat ttttgatact ttggttgttg
     8821 acggcctgta atcatactaa acaattacag gcgccaaccc gtcaatcaaa tttaaccggc
     8881 actgattttt acaagcaggc ttctgctatg aaatgggaaa gcagggattc ctttgtttta
     8941 aaagaagtgt ttgccgggaa cctgccctct tttttaaaaa aatttgcacc tgttcatgtt
     9001 tcaataagag attcagtaag tggaaagact atcagggccg tgtattttgt atctcctgac
     9061 tatttatgta tcggaacaga taatgattgg gccagaatta atattacccc tttggctgca
     9121 caaaaaattg ccgacagttt caattgtttt ttgccaaccc ggaaaatggc agatgatatt
     9181 tacaaagctg caaaagtaaa attagaaccg gttcccatgt atgcctttcg tgacagtacc
     9241 ccaaccatgt ggcaccacca cctggtaata gagggacagc gaaaaggaag agagggattg
     9301 atagcaggta taaaaaaaga tgtggttatc agtggcagga tttccaggga caaaaggccg
     9361 gatcgggttg ctatttacgg ttggcatatg ctgaacggga aacctattca gcctttatat
     9421 accgggcata tcaattggtg ggtggattac agccagggga tacgattagt gtaccggcaa
     9481 ttgaaggtgg aagggaaatg gatggattat actgttgtgc tgaaagatcc ggtattgcaa
     9541 aagcttttat gcgatgagga attttgtgat ttctaccgct atgattatta cccttaaatt
     9601 tttgtacgtt tgcaggactg gtttttaaaa tgagttgtta actatttaat taacagtttc
     9661 gacactgatt tatgcctttg ctgcgctaat ttgcaaaggt ttttttatac ccgttcaatc
     9721 attaattaat ttcagcactg gcaaagaaat ttacatacct gtttttttat ataatgctga
     9781 ttactcctgt tctgtcttct gcacaggatc caatgattca gcaaatggga aacaggatta
     9841 agcaggttgg tggaagtatg cgttctggta gtggcagcac tgacagtttg cagcgaagaa
     9901 ataaaatgga agattccatt acgatcagtt tcaggtatct tgattcaacc aagagctata
     9961 agctggattc gaccgtcaca gatttcaccc gccgctttcc gctgcctgcc acttatatta
    10021 atctgggtaa taccggtaat gccgcaaggt cattgttatt ttctcccaat tttaatgctg
    10081 gatttgatcc cgggtttcat gcctttgatg tgtataaatg gaaaatgagt gatgtcaggt
    10141 tttttaatac aacaagacct tactctgaac ttaattatat gcttggcagc cgggtggagc
    10201 aaatgattag tttgctgcat acacagaata ttaaacccaa ctggaatttt ctttttcaat
    10261 accggctgat caactctccg ggatttttcc aaaatcaaaa aaccaatcat aataactatt
    10321 tactgagttc aaagtaccag tctaagaatc tccggtacca taattatttt gtattgcttg
    10381 gcaacaaact gcaatcggct gagaacggag gtataattga tacgtcagat atattgaatg
    10441 atccgatata taaagaacgg tttaatataa ataccaggat tggtggtgta agccagtttt
    10501 cttctaattt cttttccaat aagataaaca ccgggaataa atacagtgag tttacgttat
    10561 tgctccggca gcaatatgat tttggcaaga aagattccat tgttacggat tcaacagttc
    10621 tgccattatt tttcccgagg gtaaggtttg aacataccct gcaactggat caaaacaaat
    10681 atatatacca ggataatgtt gcggattcgg tttattacaa aacttattat gatacaagtc
    10741 ttcgttatcc gaaagacact ttactattcc gggaaaggtg gcaggtgctg accaatgatt
    10801 tttcaatcta ccagtttcct gatgcaaaga acctgcaaca attcatcaag ctgggagtgc
    10861 tgctgcagaa tataagcggg gagctggcat cggggaaaga aaatttcctg aacaccgccg
    10921 gtcatgcgga gtaccgcaat aagacacgca accaacaatg ggatattgaa gcaaacggga
    10981 aattatattt taccgggttt aatgcagggg attaccaggc acatatcagt ttgcaaagga
    11041 tgctggggaa aaagctgggc tacctgcaat tagggtttga gaatatcagc cgtactccat
    11101 cttttatttt cgatagccgc agtagtttct atttgcttaa aacagtaaaa gattttaaga
    11161 aagaaaacag tactcatctc tttgcatctt attttctgcc atccttcaag ctcaggttga
    11221 cggggcatta ttacctggcc actaattata cttatttcac taattattat gaagcacagc
    11281 aggaaagtgt tttgttcaat gtattcaggt tgggccttga aaaaacgatt aagttcggta
    11341 aacgatggaa ctggcatact gatatttatt tccagcaggt ggtaggcgat gccccggtaa
    11401 atgtaccggc tatttatacc cgcaacagaa ttgcatatga gggtaatctt gggtttaaga
    11461 accttgatat tgcaatgggt gtggaattac gttaccgggc agcttataaa gccgataact
    11521 attctcccgc cttaggacag tttttctacc aggatagtat cactatccgt aaccctttgc
    11581 ccgatatttc agggtatgtg catttccgta tccgcccttt tactgcattt attcgtttcg
    11641 agaaccttaa tactggtcaa aatttagagg gattcagttt tacccgtaat aacctggtag
    11701 cccctgatta tgcaatgcca ggccttcagt tcaggatagg aattttctgg aagtttgtta
    11761 attgattctc aaaactatac gcctggaaac aaacagtttc ggttatccaa tattgctgtt
    11821 atctttgtag gagtaatgaa gaaggtaatt gcctccatat cagttctatg ctattttgta
    11881 gttacctgcg gagtagtagt gaattttcat tactgcatgg acaaactggc ttcggttcat
    11941 ttttttggag acaaaagtga taaatgtggc caatgcggga tggtagtaca tgaatctgat
    12001 ggttgctgtc atgatgaggt gaaagttata aaacttgaac aggatcagaa taaaactgcg
    12061 gcagcagatt atagtatccc ttctcttaaa gaacttgcca ttactccttc ggaatttatt
    12121 gcggcatcat tttacaatat aatggaggag tgccattttt ataaccactc accccctctg
    12181 ttatctgcac aagacaccta cctgcagatt aatgttttca gaatttgata ttcagctgat
    12241 cttttctttt agatagtcaa taaactattg acttattcat tatttgtatt tttttaaaca
    12301 taattctgag atattaaata aataatcatg aaaacattaa gaattttttc aattgttgcc
    12361 ttattaatgg ccatttcttc tctctctttt gcacaaacaa aaactgaaac cttcaatgtg
    12421 tctggtaatt gcggaatgtg caaaaccaag atcgaaaaag ctgcaaaaga tgcaggggcc
    12481 agtgctgcct cctgggatgt ggatgcaaaa caactcactg ttacctataa aagcagcacg
    12541 actaacgcag caaaaatcca gcaaaaagta gccgaggtag gttatgacaa cgcaggattt
    12601 aaggcaacgg ctgaagcata taataagctg catggttgtt gcaaatatga aagagaaact
    12661 gcagcggctt ccgaaaatca ttcctgctct ggtaaatgcg atatgaaaga cgggaaatgt
    12721 gctgacatga ctgatggtaa aaaaatggat tgtgctaagg atggcaagtg caccaaacat
    12781 gtgcatgaag gacaaactgg tgaaaaaatg gattgctgca aagacggaaa atgcagtatg
    12841 cccggtcatg acggtaaaga ttgttgtaag aaagatgatg ccaaaatgga ttgttgcaag
    12901 gatggaaaat gcagcatgcc tggtcacgat gggaaagatt gctgcaagaa atcataacat
    12961 ttaagaccgt taaatagtag atatgaaaaa agtgttgttg ataatcacaa tggccttttt
    13021 cgcactgcaa tcctatgccc agttttcaaa agccaggctg caggccacgg ggttaacctg
    13081 tgccatgtgc agtaatgcga taaataaggc cctggaaaag atatcttttg tagaatctgt
    13141 aaagtcagat attaagaatt ctgcattcaa tattgaattt aaacagggtg ctgatgtaaa
    13201 tatggatgcc ttgaaagatg cagtggaaga tgcaggcttc tttgtgggag gcttccaggt
    13261 aacaggtgtt tttactgaaa tgaaaattga tcctgataaa tatgcaaaga ttggaaatta
    13321 taattttcat ttcctgaatg taagctcccg gattttgaat ggggaaatta ccctcaaagt
    13381 gctggataaa tcatacctga ccgcaaaaga gtttaagaaa ttaagttctt ctgtaaaggc
    13441 tccctgctta caaacaggta aagcagatgg aacacatgta aaagatggtg taacagcagg
    13501 tgaaagagtt taccatgtta caatctgatt ataaatgtat agtttctctc cttccttttg
    13561 ggaggagaga aatttttcaa actttatttc tatgaaaata ttccggcaat atttcattat
    13621 tcttttttct cttttttttt cggtagatgc gatagcgcag gttaaatcgg gatataaagc
    13681 acctgaagtg gctttacctt ccaccaccgg agacacaatc ctgctatctt ctttgaaagg
    13741 gaaagtggtg ctgcttgatt tctgggcatc ctggtgcaag ccatgccgtg cttctaataa
    13801 gggacttgtt aagctgtatc ctaaatttaa agataagggc ttcgaaatac ttggggtatc
    13861 gcttgacgag gacaaggctg actggaaaaa agccatcaaa caggataaaa taaactggct
    13921 acaggtaaat gacccgggcg gctggcaatc tccgactgtt ttgcagtgga ggataaatgc
    13981 aattccaaca tcttacctca ttaacaggaa tggtgaactg atcgctatgg acctggaggg
    14041 caaagaactg gagaaggcac taaaggaaat tttaggtaac tgatcaaaac aaaatcatgc
    14101 ttcggtttct tttttctttt ttattattat ttgcgcttcg attaagcggt caggagctat
    14161 atgtgtttag tgagcctgcc tccaatgtac ctgcacattc catgagtata aagctcaaag
    14221 agcattttgt taccagtgat aggatttatg aaaggtttgc cggcaggttc atgccacaaa
    14281 taatgtttgg tgtaagtaag aagttaatga tcagggcagg agcatccatt gcaaatatgc
    14341 atacggataa ttcaaggttt gaatctttta gtttttatac caaatacagg tttctgtcaa
    14401 aagatgagtt gcataaacat tttcgtatgg ctgtttacgc agaagcttcc aggacaaatg
    14461 cgccttttca ttatgatgag atttccatga tgggcgataa gagcggggtg gaattgggta
    14521 ttataacaac acagttatgg aacaagtttg ctttatctgg aacagtaagt catacgcagg
    14581 tgcttgataa gtcaagaaat gataaagtgc tttatatacc cgaaagaaac taccagtcag
    14641 ttaatttttc cttatcaggc ggatacctgc tattgcccag ggagtatact gactataagc
    14701 aaaccaatct taatctttac ctggaaatac tcggacaaca gacgtttgaa aacccttctt
    14761 actatgtcga catggcgccg gcaatacagc tgatctttaa cagtaataca aaactgaata
    14821 tgggctaccg ctttcagttg ggtagcgata tgcagcgaat ggctgctaac agttggctcc
    14881 ttagtattga aagaaccttt ctgaatgctt tgaaaaagag gtagtgggtt cttcgcttta
    14941 aaatgataat tatgaagatc ttaaaaagag atatattttt tgctgccttt gtaatcatac
    15001 agctggcagg cactgatatc tatgcacagg aatctgctgg aaattcaaca gacacagtcg
    15061 tagttataaa tgtggatgga gcctgtgaaa tgtgcaaaga acggattgag acagtggcaa
    15121 aggataaaaa gggggttcgt gatgctgttt gggatattga gtccaaaaaa ttaaaactgg
    15181 tttataaccc tctgcttatt acagttgaaa aaattcagtg cagtattgct gatgccggcc
    15241 atgataatga ttacgaaaag gcaaaggata aagtatatga gaaattacca gcttgttgtc
    15301 gttacagggg aataaatcat cccaaagaaa agccaggtga aaacaacctg aataacaatg
    15361 cggctaatcc tgtagttaca caagttgtaa aaggtgtggt gctggaagaa aataaacagg
    15421 gatcatttgt tccgctgatg ggtgcttctg tattatggtt aggtactaat acaggtgtag
    15481 ttacagatac agccggtgtt tttattatta aaaatgaaaa agggtacggc agattagtaa
    15541 tcagttatac aggttatgaa ccagatacat tagttgtaac gaatgcagaa gagttaaaga
    15601 tcattcttgc atccggccgg cagctgaatg aagttacagt ttcagcaaag cagcgttcta
    15661 cttatttgtc ttcacttaat ccaatccgga cacaagtaat gacagagagg gaacttttta
    15721 aagctgcctg ttgtaacctg agtgaaagtt ttgaaaccaa tccttcggtt gatgtgtcct
    15781 acaacgatgc agtgaccggt tctaaacaaa ttcagttatt ggggctaagt ggcaactata
    15841 cacaactgac tgttgaaaat ctacccgggc caagggggct ggctactcca ttcgggttga
    15901 attttattcc gggtccctgg gtagaatcta tccaattgaa taaaggagtt ggatcggtgg
    15961 taaatggata tgagagtatt gccgggcaga tcaatatcga attaaaaaaa ccggcaacag
    16021 cagaaaagtt atatctgaat gcgtacatca atgatatgac caaaacagat ctgaacctgg
    16081 tactgactga aaagtttgga aagaactgga gcaccgcatt attattacat gataattttc
    16141 aaaccaaaaa gaatgtcgat tataatgatg atggtttccg cgatatgact acaggtaatt
    16201 tgtttactgc tgttaatcgg tggaactata acggtgggaa aggctttgaa tcacaattgg
    16261 gttttaaaat attgaatgat gaccttacag gagggcaaac ttcatttgat ccggataagg
    16321 acaaatttac tacccgtagt tatggacttg gaatcaatac caggaggtat gaggtctttg
    16381 ccaaagccgg gtatgttttc cccgggaaaa agtataaaag cataggcctg cagttatcag
    16441 cgtttgatca taagcaggat tcttattttg gtatgacatc atacaaaggg cggcaaaaga
    16501 atttttatgc caacctcatc taccagtcga taattggaac cacggcacat aagttcagaa
    16561 cagggatgag tttcttatca gataattatg atgaattatt caattcaata aagtatcaaa
    16621 ggcaggaaac agttcccggt gccttttttg aatatacttt taccccggtt gaaaaattta
    16681 atgtagtggc gggaatgcgg gcagatcata acagcttgtt cggctggttt gccacaccaa
    16741 gattacatat caggtacgag ccggttaccg gaaccactat tcggttaagt gctggtcgtg
    16801 gacaacgtac tgcaaacatt tttgcagaga ataatagtgt atttgttagt tcaaggcaaa
    16861 tgaatatttt gactgcatct gcaggaaagg cttacgggct tgatcctgaa attgcctgga
    16921 acaaagggct aagtgttgat cagaaattca agctttttaa gcgaaatgcc aatttgggac
    16981 tggatttttt ccgtaatgat tttcaacagc aggtaatagt tgacctggaa gatgcgaggc
    17041 aggtgaaatt ttacaatctt gatggaaaat cttattccaa tagcttccag gcagaaatga
    17101 atatggagcc agccaggaaa ctggaggtca ggctggccta ccgtttcttt gatgtgaaag
    17161 ccacatatgg gaatcaatta ctccaaaagc catttactgc caagcacagg gcattcacca
    17221 atttggctta tgaaataagt agctggaaaa tagattatac gtttaattac aatggttcta
    17281 agaggattac taatacgacg gctaacccgg ccatttacca gcggcaggtt aattctgatt
    17341 cgtacatatt aatgaatgcg caggtaacta aaaccgttgg taaaaagaac
//
LOCUS       DAORTI010000070        17324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_2972_length_17324_cov_8.617812, whole genome shotgun sequence.
ACCESSION   DAORTI010000070 DAORTI010000000
VERSION     DAORTI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17324
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_17324_cov_8.617812"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLO70_09930"
     CDS             complement(<1..615)
                     /locus_tag="PLO70_09930"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQZ74828.1"
                     /translation="MKRYINLFIAIAGGLLLWAAWPASSLSVIIFFAWLPLLWLESKI
                     ESRKKFFGLTYVTMFIWNVSTTWWIWNASAPGAVSAFLANSLLMCLPWLGFKIAKKWL
                     GELPGYIALIAFWMCFEYIHLQDWGLSWPWLTLGNAFAIHPEWIQWYEYTGTSGGSLW
                     ILVVNIFLFRHVKNNFTREGTKTYKNLVAGILILIAPVAFSLLGF"
     gene            736..1467
                     /locus_tag="PLO70_09935"
     CDS             736..1467
                     /locus_tag="PLO70_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617276.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQZ74829.1"
                     /translation="MKNYATAIIIAIAIIAGVLLLGNAYKYKYKTTETITVTGLAEKD
                     FVSDQIVWTGDYSRKTMDLKSAYAQLKVDESKIRNYLQGKGVTDAEMVFSAVSIDKEF
                     SNRYDENGRITGSEFTGYNLKQKVTVESKNIEKVEKISREVTELIESGIEFNSSSPSY
                     FYTKLSELKIDLLAKASADAKQRATTIAKNSGSGLGSIKKANMGVFQITGQNSDEDYS
                     YGGVFNTTSKNKTASITIKIDYAVK"
     gene            complement(1547..2218)
                     /locus_tag="PLO70_09940"
     CDS             complement(1547..2218)
                     /locus_tag="PLO70_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74830.1"
                     /translation="MHKIITVLSAFLFLSVFTGCIEVIEELTVTKDGKGHYRKTMDMS
                     QLLSMKEMLGGAAKEGTDSTTNSGTENSDNPAESIAKEWYAIKNMEGLSNVTIDTAGY
                     VFTLNYDFESETALNKAIAAESKDSPQANVYNLAEKSVSRTGLGSTGDLLGEGEDDEN
                     MEMMKTFFSDMKYKLVINLPGRVKSVSNKKATIENNKTVKLETNLKDLVEKTSSLEMK
                     IDFKN"
     gene            complement(2316..3809)
                     /gene="guaB"
                     /locus_tag="PLO70_09945"
     CDS             complement(2316..3809)
                     /gene="guaB"
                     /locus_tag="PLO70_09945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQZ74831.1"
                     /translation="MATRKSSPHPNSKKFFGEGLTFDDVLLMPGFSQVLPRDVEIRSR
                     LTKNITLNVPLLSAAMDTVTEASLATALAREGGLGILHKNMPIDKQAEQVRKVKRSES
                     GLIIDPVTLLEDATIGDALRLMRENKIGGIPIIDKTRKLVGILTNRDLRFEDNPKRKV
                     SEVMTKENLYTAPEGTDLKKAEQLFKKTKVEKLPIINKQGQLTGLFTYSDILKLKSHP
                     NAVKDAFGRLLVGAGVGITKDLLDRVAALQAVGADIIALDSAHGHSKGVIDALKNVKK
                     NFKGISVIAGNVGTAAGAKALAEAGADAIKVGIGPGSICTTRIVAGAGVPQLTAIMEA
                     ASVLKPKNIPLIADGGIRYTGDMVKALAAGADCVMMGSIFAGTEESPGETIIYEGRKF
                     KEYRGMGSLGAMATGSSDRYFQDVEDDVKKFVPEGIEGRVAYKGTLKEIVYQYTGGLR
                     AGMGYCGAKNIAELQQATFVKITNAGMRESHAHDIEITKEAPNYSRR"
     gene            complement(3854..5881)
                     /locus_tag="PLO70_09950"
     CDS             complement(3854..5881)
                     /locus_tag="PLO70_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ74832.1"
                     /translation="MRLSYKFILSLFVVFLYAENSLFAQACTTLGQTPSTAFPVCGTT
                     VFTQASVPLCATNSLFVPGCSGDGANYENRNPFFYRFTCFVSGTLGFLITPLAANEDY
                     DWQLYDITGRDPNEIFTNNSLVVTGNWAGTYGPTGASASGVPGIQCASDPTAGANTFA
                     TMPNLVQGHEYLLLISHFTNTQSGYNLSFGGGTAVITDPTEPHLQSAKPDCDGTKITV
                     KLNKKVRCNSITLTGTEFSLLPAVTTVVAAVPDSCAFAFDFDEITLTLAAPLTNGNYQ
                     LVINNGTDANTLLDNCARAIPQNEQVPFTYAVPQPIFADSIGRVGCASDSIKIYFPKK
                     ISCASIAANGSDFSVSGPAPLTILSASGNCVDGKTEYVVVKFTTPVYTGGTYQLSLKA
                     GNDGTILVDECGQEMPIQTLPFITADTVNASFTYTAVYGCQRDTFLFSHNGAHNVNSW
                     NWTINTITATTQNHTIIFPATSTNDIQLIVSNGVCKDTATTQVVLSNEVKASFTMPDI
                     ICPEDPLEVVNTSTGQISSWLWKYDVVGSSTVKDPPPFLFPTINREAYYTVKLLAYNS
                     SLGCADSARKTLTVLDHCLIEVPSGFTPNNDGLNDFFQPHNALKADNYEFKVYNRWGQ
                     LVFQSRNWREKWDGKINGAYQTTGVFVWMLSYTHRDTGKQVFRKGTVTLIR"
     gene            complement(5940..7046)
                     /gene="dprA"
                     /locus_tag="PLO70_09955"
     CDS             complement(5940..7046)
                     /gene="dprA"
                     /locus_tag="PLO70_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQZ74833.1"
                     /translation="MHPDLLYQLSLTMVPNIGPVQAKILLQHCEAEEIFHAKKLFLEK
                     IEGIGPVRADSIKKFDGFYRAEDEIKFIEKYRIKPLFLTDEAYPKRLLNCYDSPTLLY
                     YRGDADLNAPKIISIIGTRSHTDYAKQATEKIIKELAGLNVAVISGLAYGVDAIAHKA
                     ALKNNLPTVGVLAHGLDHIYPPEHTHLAKEMVKNNGGLLTEFRSNTKPDKHNFPTRNR
                     IVAGMSDATLVIETGLKGGSMITAELANGYNKDVFALPGKITDAKSAGCNFLIKNNKA
                     MLLTDAQEIIDVMGWEEKKNRKNEKKNQRELFIELTADEKIIVDILKEKASVPIDEIN
                     SKSGLSSSAVAGAILNLELQSVVLSLPGKLYRLA"
     gene            complement(7095..9014)
                     /locus_tag="PLO70_09960"
     CDS             complement(7095..9014)
                     /locus_tag="PLO70_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ74834.1"
                     /translation="MKRLLLFFACCSLFQYSFSQPYDPEKVNKKAVQFFDQAYERAQD
                     GNYPNAIGLLLQAVKTDTKYVDAYLALGNIYGILKNYKSAIGYYEKAFAIDSGYTLQD
                     KLPYSINLAGLGEFERALAAVNELLKKKPPKNPVTLENAYKRKRSYEFAVEYASNNQQ
                     KNYVFAPQNMGDSVNSNESEYFPCLTIDGNQLAFTRKLNGRNEDFFTSSKTPSGNWGV
                     AKPMEGNVNTEQSEGAQNISQDGQWLVFTGCYRPDGMGGCDLYISYKTENGWSEAMNL
                     GGTINSDQWESQPCLSPDKNELYFVSRRFGGYGGSDIYVSRMQPNGKWGNPENLGPGI
                     NSPADEQCPFIHADNQTLYFTSDHWPGYGDEDLFYVRKQPGGRWSKPVNLGYPVNTID
                     REGTLFIAADGKTAYYASDRSDSKGGLDIYSFELRENIRPAKTLWVKGKVYDKKTTNG
                     ISAAVELIDLATKQVLTKVQTSESGEYFITLPVGKDYAFNVTRKGYLFYSDNFSMSNR
                     SPDSIYEKNIGLQPIEANASIVLKNIFFDVNKFELKPASQAELDILVQLLAENPSVKI
                     EISGHTDNVGQPADNLTLSINRAKAVVSYLVNKNISAQRLIAKGYGETKPIADNKSDE
                     GRAENRRTEMKVVSQ"
     gene            complement(9097..9717)
                     /locus_tag="PLO70_09965"
     CDS             complement(9097..9717)
                     /locus_tag="PLO70_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQZ74835.1"
                     /translation="MNEVLKETKMLPVMEHFYTLQGEGFHQGRAAYFIRLGGCDVGCV
                     WCDVKDSWNAENHPKFAIKDIVAAIKKTPAQLVVITGGEPLMHNLDELTNQLKAAGLQ
                     TNIETSGAHPLSGSWDWICLSPKKFKAPLPEILPRANELKVVIFNKSDLEWAEKYAAL
                     VSPSCKLYLQPEWDKAAQMIPLITDYIKAHPAWELSLQIHKYINVP"
     gene            complement(9753..10628)
                     /gene="folD"
                     /locus_tag="PLO70_09970"
     CDS             complement(9753..10628)
                     /gene="folD"
                     /locus_tag="PLO70_09970"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQZ74836.1"
                     /translation="MQILDGKKASQAIKDELKIEVAQLTAEGKKIPHLAAILVGDSGA
                     SATYVAAKVKACEEVGFKSTLIHFEDTISETKLLDKIKELNTDPDVDGILVQLPLPKN
                     ISDEEVINAIDPDKDVDGFHPINVGNMVQGLPTFVPATPHGIMLLLEHFKIETKGKHA
                     VVVGRSNIVGRPMSILLSSNTNPGNCTVTLCHSQTKNLKDFCLQADIIVAALGKPEFV
                     TADMVKEGAIVIDVGITRMEDATKKSGFKLKGDVNFETVAPKCAWITPVPGGVGPMTI
                     AALMKNTFRSCMQKN"
     gene            10738..11568
                     /locus_tag="PLO70_09975"
     CDS             10738..11568
                     /locus_tag="PLO70_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74837.1"
                     /translation="MYRKFSLLLLIVFSISKMPEAQTLRRPQTAGYTGLGAYSLSHTD
                     IFSFTANQASLAQLKNAAVGVYGERRFLLSELNNYTATAGLITTSGNFGLKAAYSGFS
                     DYNETQLGLAYGRKLGSKIDVGAQFNYNGIRIAEYGNAAALSFEIGVIMHLTDKLHTG
                     IHVNNPVGGKFGKDQQEKLSSVYAVGFGYEASEKFFVSAEIEKEEDQPVNVNAGIQYK
                     FIPQLLVRAGLSTATSSAWAGLGLSIKSFRLDVTTSWHPQLGVTPGLLVLFNFNKKED
                     "
     gene            11565..13601
                     /locus_tag="PLO70_09980"
     CDS             11565..13601
                     /locus_tag="PLO70_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQZ74838.1"
                     /translation="MRSAVLILLTILLSLYSYAQEIPVNTEQQLENLADTDQAETEDD
                     SYLQELEYFRKNPLNLNNAEAEELKLLRILTDLQIANLVAYRKLFGKLISVYELQAVP
                     AWDIITIRKLLPFITVSSSLSLPEAGKRFKDGEHSLLLRLSQVLERSKGFDNAITGTK
                     YLGSPQRLFLRYRYTYKNLLQFGLVGDKDAGEQFLKGAQRYGFDFYSFHLFARKIGVI
                     QALAIGDFTVNMGQGLIQWQSLAFKKSVDVMGVKRQAAVLRPYSSAGEFNYHRGAGIT
                     IRKGSFETTVFASLRKVSANFVADTVNNEEYISSFLTSGYHRTNSEITDRNRITQTAF
                     GGNIIYRGNRWHLGVNSIYYNFSFPVQKRDEPYNLYAISGKNWYNFSIDYSYTYKNLH
                     LFGEAASDKNFNKAFINGLLISVDPNVDLSFVQRTISAAYQSVNGNAFTENTYPTNES
                     GFYAGISIRPGIGWRIDAYSDIFKFPWLKYLVDAPGSGKDFLAQLTYTPNRQAEMYTR
                     FRNETKKGNQPGNLTTTNFLVSLPRQSWRTQVNYKLNTTISLRNRVELMWFDRKGENS
                     EKGFLTFVDVVYKPMLKPFSGIIRLQYFETDDYNSRIYAYENDVLYSYSIPAFFDKGF
                     RYYISLNYDWGKKLTFWLRWAQTIYKNRDTVGSGLDEIPGNRRSEVKFQVRVLF"
     gene            13775..16894
                     /locus_tag="PLO70_09985"
     CDS             13775..16894
                     /locus_tag="PLO70_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQZ74839.1"
                     /translation="MRKIASLFTMLMLFSALAFGQNRTITGTVTDETGAPLPGASVRI
                     EGTRTGVAADNNGQFRILAKTGDVLVVSGAGLETTTLTVGAGNTVSITVNRLVLTGTE
                     VVVTAMGQTRQAKELGYATTKVKAAELTQAKAVNLQNGLTGKVSGLNIQTVNNGVFAD
                     TRITLRGIRSLTGNNQPMLILDGVPIALGYLSSINPNDIVDVTILKSASATAIYGPDG
                     ANGALVVTTRKGSKTKPQVSVGHTVQIERVSYMPELQNQFGSGSSIDPFGYGVYDPIE
                     NQCYGDEFDGSMRQIGRDGPNGEQYLVEYIARPEEKRKFWNTGITNQTDVSFSTGDFY
                     LSAQNVLIQGIMPKDVNRRVSVHMAGNKEYNRFKASYSINYTKGNYDVTAGSSFGNGR
                     DYTVYWNLINTPQQIPITRFKDWRNNYWANPNGYFNDYYFNPYFMIDNFRSVGRTEDI
                     FGNFELNFKATNWLTFTYRLGATISNGNAKSRAEAFTYSDFAKASGKSNAQSGDVVAQ
                     VGDGISTSSRINSEFFATIRKKAGNFDFNALLGQSFREDNSKNINVSSNNLAFPTLFN
                     VIGRKGEPGASESNFKSRLQRFFGKLSVGYNNWAFAEVTGSYDMNSRLAYYYDFDFKA
                     ISFFYPGASLSVLLSEAIPAIKNSKVISYFKIRAAKSKTGNVNLGVQALENTYGLGGG
                     FPYGSLVGYTSGNVLRLPKYTPEFVKNNEIGFELGLLKNRINLEATYYTQNNSDQIVQ
                     VQYSGATGFTSALLNAASFTNDGFEFDLRLTPLVKIRDVSIDFKANYTYQTNEVTQII
                     EGVDELGIGNANYVIVGKPAYTFQLTDYVRDDQGRVIVNSSTGLPTVDPVIKPFGHTQ
                     PNHLIGLNLNISWKDLTFSAVADYRGGAQIYTGNLGTGMDFSGISKRSAQNSRQPFIM
                     PNSSYWDGSKYVDNTSIYMTGGYNFWSQATNQNANTNYLVSGDFWKIREMSLSYNIPA
                     KVFGFTKNAIKGATFTLSGRNLFMFLPKTNEWTDPEFANTTGNAQGVSGLDNTPPTRI
                     FGATLNLNL"
     gene            16918..>17324
                     /locus_tag="PLO70_09990"
     CDS             16918..>17324
                     /locus_tag="PLO70_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74840.1"
                     /translation="MKLKKLIYGVPLAVILIAVGSGCRKSQFNINQNTNNPTDSTVSY
                     DVVLPAALHATGGIVGTSWGPIQNWMGFWARSGTYAPNVLEETYEITTGFGNGIWNNC
                     YDNNYDYQIVQNKASVAGAGFYAGIARIMKAHN"
BASE COUNT         4880 a         3584 c         3586 g         5274 t
ORIGIN      
        1 aaacccaagt aatgaaaaag caaccggagc aatcaggata agaatgccgg ctaccaggtt
       61 tttataagtc ttagtaccct ccctggtgaa attatttttt acatgccgga acagaaaaat
      121 attaactact aatatccaaa gggatcctcc acttgtaccg gtatactcat accattgaat
      181 ccattcagga tgaatggcaa aagcattgcc aagtgttaac cagggccagc taagccccca
      241 atcctgtaag tgtatatatt caaaacacat ccagaaagca ataagtgcaa tatagccggg
      301 taattctccg agccattttt tagcgatctt aaaacccagc cagggcaggc acatcagcag
      361 gctgttggcg aggaaagcag aaacggctcc cggagccgat gcgttccata tccaccaggt
      421 ggtagataca ttccagatga acatggttac atacgttagc ccgaagaatt ttttccggct
      481 ttctatttta gattccagcc agagtaaagg gagccacgca aagaaaatta ttacactaag
      541 tgatgaggcg ggccaggctg cccagagtag caatcctccg gctatggcta taaaaaggtt
      601 gatatatcgt ttcaagagtt ggttttcaga atgaaaaata atgtaaagaa agaatttctt
      661 ttttgacttt aacgtaatat atagtaatct aatttataat tttatagtat caattaccat
      721 taacacctgc acatcatgaa aaattatgcc accgccatta tcattgctat cgccattatt
      781 gccggcgttt tgttactggg caatgcatat aaatacaaat ataaaaccac tgaaacgatt
      841 actgtaactg gtcttgcgga aaaagatttt gtaagtgacc agatcgtatg gacaggcgac
      901 tatagccgta aaacaatgga tctgaaatca gcgtatgcac aattaaaagt ggatgagagt
      961 aagatccgga actacctgca gggaaagggt gtgacagatg cggaaatggt cttttctgcc
     1021 gtatctattg ataaagagtt ttcgaaccgc tatgacgaaa acggaaggat aactgggagc
     1081 gaatttaccg ggtacaacct gaagcagaaa gtgactgttg aatcaaagaa tattgagaaa
     1141 gttgaaaaga tttcgaggga agtaacagag cttattgaaa gcggtataga gtttaattca
     1201 tcttccccat cttattttta taccaagctg tctgaattaa agattgacct gcttgcaaaa
     1261 gccagtgcag atgctaaaca aagggcaact acgatcgcaa aaaattccgg cagtggatta
     1321 ggcagtatca aaaaagctaa tatgggggtg tttcagatca cgggtcaaaa cagcgatgaa
     1381 gattacagct acggcggggt atttaatact acatcaaaga ataaaacagc atcaatcact
     1441 attaaaattg attacgcagt aaaataattt ctttcaataa taaaacagcc tgccatataa
     1501 taatggcagg ctgttttagt taaatgtatt tgttggggat gcgatattaa ttcttaaaat
     1561 ctattttcat ttccagggag cttgtttttt ctaccaggtc tttcaggttt gtttccaatt
     1621 tcaccgtttt attattttca atggtagctt ttttatttga cactgatttt acacgtccgg
     1681 gcaggttaat taccagcttg tatttcatat cactgaaaaa cgttttcatc atttccatat
     1741 tttcatcatc ctctccctcg cccagcaagt cacctgtact tcccaatcct gtcctgctca
     1801 ccgacttttc tgctaaattg taaacatttg cctggggact gtctttgctt tctgcagcaa
     1861 tagctttgtt cagtgctgtt tcactttcaa aatcataatt aagggtaaaa acataaccgg
     1921 ccgtatctat agtaacatta ctgagtcctt ccatattttt aatagcatac cattctttgg
     1981 caattgactc tgcggggtta tccgaatttt ctgttccgga attagttgtt gaatctgttc
     2041 cttctttggc ggctccgccc aacatctctt tcatgctcag taattggctc atatccattg
     2101 tttttctata gtggcccttc ccgtcttttg tgacagtcag ttcttctata acttcaatac
     2161 agccggtaaa aacagataag aaaaggaaag cggatagtac ggtaataatt ttatgcatat
     2221 tatattttct atagggtgaa acgaattaat aaaaaaatct gtgctgcaag atagcgtcac
     2281 agattgataa agccaaaccg gggattaatt tattattatc tcctgctgta gttaggtgct
     2341 tcttttgtta tttcaatatc atgagcatgg ctttctctca ttccagcatt ggtgatcttg
     2401 acaaatgtgg cttgttgcag ttcagcgata tttttcgcac cgcaataccc cattcctgcc
     2461 cttaatccac ccgtgtattg ataaactatt tctttcaatg ttcctttgta tgcaacccgg
     2521 ccttcaattc cttcgggtac aaatttcttc acatcgtctt ctacatcctg gaagtaacga
     2581 tcactgctgc ccgtagccat agcacccaaa gaacccatcc cacgatattc cttgaatttc
     2641 cttccttcgt agatgattgt ttcaccagga ctttcttcag taccggcaaa tatgcttccc
     2701 atcattacac aatcagcgcc tgcggctaac gcttttacca tatccccggt gtagcggatg
     2761 cctccatctg cgatcagggg aatatttttt ggttttaata cggaggctgc ttccattata
     2821 gcggttaatt gcggtacacc agcacctgcc acgatcctgg ttgtacaaat ggaacccgga
     2881 ccgataccga cttttattgc atcagccccg gcttccgcta aggctttggc tccggcggca
     2941 gttcctacat taccggcaat aacactgata cctttgaaat tcttctttac atttttcaac
     3001 gcatcaatca cacctttact gtgcccatgt gcactatcca gtgcaataat atctgcgcct
     3061 acagcctgta aagcagcaac cctgtctaac agatctttgg taatgcccac accggcaccc
     3121 actaacagtc ttccaaatgc atcttttacc gcattaggat gacttttcaa tttcaaaata
     3181 tcactgtagg taaaaagccc tgtcaattgg ccctgcttat tgatgatagg cagtttctcc
     3241 actttggttt ttttgaataa ctgttccgct tttttaagat cagttccttc aggagcggta
     3301 tacagatttt cctttgtcat tacctcactc acttttcttt taggattgtc ttcaaaacga
     3361 aggtcccgat tggtaagaat gcctacaagc ttacgggttt tatcaataat aggaataccg
     3421 ccaattttgt tttcccgcat cagtcgtaat gcatccccaa ttgtggcatc ttccagcaaa
     3481 gtgacaggat caatgatcag cccgctttca cttcgtttta cttttcttac ctgttcagcc
     3541 tgcttatcaa ttggcatatt tttatgcagg ataccaagac caccttcacg ggccagggcg
     3601 gtcgccagtg acgcttctgt tacagtgtcc atggcggccg aaagcagggg tacattcagc
     3661 gtaatatttt ttgtaagccg gcttctgatc tccacatcac ggggtaatac ctggctgaag
     3721 ccgggcatca gtaatacatc atcgaaggtg aggccttcac caaaaaattt ctttgaattg
     3781 gggtgggggg aagattttct tgttgccatg tgcaaagata gcccgccggg taattatctc
     3841 ccaaatgaaa ttatcagcgt atcagggtga cagttccttt acggaaaact tgttttccgg
     3901 tatcccggtg ggtgtaactg agcatccaga caaaaactcc tgttgtctgg taagcgccgt
     3961 tgatttttcc atcccatttt tctctccagt tccgggattg aaatacaagt tgcccccatc
     4021 ggttatacac tttaaattca taattatcgg ctttcaatgc attgtgcggc tgaaagaaat
     4081 cgttgaggcc gtcattattg ggggtaaatc cggaaggcac ttcaatcaaa caatgatcaa
     4141 gtactgtaag tgtttttctg gcactgtctg cacaacccaa tgaactatta taagcaagca
     4201 atttaacggt atagtaggct tccctgttaa tagtcggaaa aaggaatggc ggtgggtctt
     4261 tcactgtgct gctgcccacc acatcatatt tccacaacca ggaacttatc tgtccggtac
     4321 tggtattgac cacttccagc ggatcttccg gacaaataat atccggcata gtgaaggaag
     4381 cttttacttc attgcttaat accacctgtg ttgtcgcagt atccttacat acaccattgc
     4441 taactattaa ttggatatca tttgtgctgg tagcaggaaa aatgattgta tggttttgag
     4501 tggtagctgt tatagtattg atggtccagt tccagctatt gacattgtgt gcgccattat
     4561 gtgagaataa aaaagtatct ctttgacacc catacacagc ggtataagta aatgaagcat
     4621 tcacagtatc cgcagttata aacgggagtg tttgaatcgg catttcctgt ccgcattcat
     4681 caaccagtat ggttccgtca tttcctgctt ttaaactgag ctggtaagtt ccgccggtgt
     4741 atacaggagt ggtaaatttt accaccacat attctgtttt cccatcaaca caattaccgg
     4801 aagcagataa aatggtaagc ggagctgggc cggaaactga aaagtcactg ccatttgcag
     4861 cgatactggc acaactgatc ttttttggga aataaatttt tattgaatcc gatgcacaac
     4921 ccacacggcc aatgctgtca gcaaaaatgg gctgtggtac agcatatgta aatgggacct
     4981 gttcattttg cggaatggcc cttgcgcagt tatctaataa tgtatttgca tcggtcccat
     5041 tattgatcac cagttggtaa ttgccgtttg ttaacggagc tgccagtgtt agtgtgatct
     5101 cgtcaaaatc gaaagcaaag gcacaggaat ccggtaccgc agcaacaaca gtggtgacag
     5161 caggtaaaag gctgaattct gtgcctgtca atgtgatact gttgcacctt acttttttat
     5221 tcagtttcac tgtgatcttt gttccgtcac aatcaggctt tgcgctttgc aggtgtggtt
     5281 cagttggatc tgtaattact gcagtgccac caccaaaaga taaattataa ccactttgtg
     5341 tattggtaaa atgactgatg agtaaaagat attcatgccc ctggaccagg ttgggcattg
     5401 ttgcaaaagt attggctccg gctgtcgggt cggaagcaca ctgaattccg ggtacaccac
     5461 tggcggaagc accggttggt ccatatgttc ccgcccaatt acctgtaact accagcgagt
     5521 tattagtaaa aatttcattt gggtctctcc cggtgatatc atacaactgc cagtcataat
     5581 cctcgttagc tgcaagagga gtaattaaaa aacccagtgt tccggaaaca aaacaggtaa
     5641 aacggtaaaa gaaaggattc ctgttttcat aattggcgcc atcgcctgaa cagccgggaa
     5701 caaataagct gttagtggcg caaagcggaa cactggcttg tgtaaagaca gttgtaccgc
     5761 aaaccggaaa ggcggtagaa ggtgtttgcc ccaaagtagt acaggcttga gcaaacaatg
     5821 aattttccgc atatagaaat acaacgaaaa gggaaagaat gaatttatat gataacctca
     5881 ttgtactata ctgtagttga atattatgac aacaattcag gctgatatgt tgcctgaaat
     5941 caggcaagac ggtaaagttt gcccggcaaa gatagtacca cactttgtaa ttctaagtta
     6001 aggatggcgc ctgctactgc actgctgctc aatccgcttt tactatttat ttcatcaata
     6061 ggaacggatg ctttttcttt caggatgtca acaattattt tttcatctgc agtaagttca
     6121 atgaataatt cccgctgatt ttttttctcg ttctttctgt ttttcttttc ctcccagccc
     6181 atcacatcta tgatctcctg cgcatctgtc agcaacattg ctttgttgtt tttaataaga
     6241 aaattacaac cggcgctctt cgcatctgtt atttttccgg gcagggcaaa cacatcttta
     6301 ttataaccgt tggctaactc tgcagtgatc atacttcctc ccttcaatcc tgtttctata
     6361 accagcgttg catcactcat gcccgccaca atccggttgc gggttggaaa attatgttta
     6421 tctggtttgg tattacttct gaattctgtt aataatccac cattgttttt taccatctct
     6481 ttcgcaagat gggtatgttc cggagggtag atatgatcaa gcccatgtgc caatacgcca
     6541 acagtcggga ggttgttttt aagggccgct ttatgcgcaa tagcatctac accataggca
     6601 aggccgctga ttaccgccac attcagtccg gctaattctt taataatttt ttcggttgcc
     6661 tgtttggcat aatcagtatg acttcttgtg ccgatgatgg aaatgatttt tggagcattc
     6721 aggtctgcat caccccggta atagagaagg gtgggggagt catagcaatt cagtaaacgt
     6781 ttcgggtatg cctcatcggt gagaaacaat ggcttgatcc tgtatttctc aataaacttt
     6841 atttcatctt cggccctgta aaagccatca aattttttaa tagagtctgc tcttaccggc
     6901 ccgatacctt caatcttttc gagaaataat tttttagcat gaaatatttc ttctgcttcg
     6961 caatgttgta ataatatctt cgcctgtacg ggaccaatat tgggtaccat cgttagcgaa
     7021 agctggtaaa gcaggtcagg atgcatagca ggataagtat ggcatcaaat ataagcggaa
     7081 cccgccgttt ttgattattg actgaccact ttcatttctg tacgccggtt ctctgctctt
     7141 ccttcatcgg atttattatc tgcgatgggt tttgtttcgc cataaccttt ggcaattagc
     7201 cgttgggctg aaatattttt attgaccagg taactaacca cagcctttgc ccggttgata
     7261 ctaagcgtta agttatccgc tggttgtccc acattatcgg tatggccgct gatttcgatc
     7321 tttacagacg ggttttcggc gagcaattgc accaggatgt ccagttcggc ctgtgatgct
     7381 ggtttcagct caaacttgtt cacatcaaag aaaatatttt ttaacacgat actggcattg
     7441 gcttcgatag gttgaagccc gatattcttt tcatatatag aatcaggtga gcggttgctc
     7501 atggaaaaat tatcagagta aaagaggtaa cctttccggg ttacattaaa tgcatagtct
     7561 ttcccaacag gtaatgttat aaaatactca ccggattcgc tggtctgtac ttttgtaagt
     7621 acttgtttag tggcaagatc aattaattca actgctgcgg agataccatt cgtagttttt
     7681 ttatcatata cttttccttt tacccatagt gttttggccg gacggatatt ttctctcagt
     7741 tcaaaactgt aaatatccaa acctcctttc gaatcgcttc tgtcgctggc atagtaggcg
     7801 gtctttccat ctgctgcaat aaataaagtt ccttccctgt caatcgtatt aacaggatag
     7861 ccaaggttta ccggtttgct ccacctgcca ccgggttgct tacgtacata aaagagatct
     7921 tcatcgccat aaccaggcca gtgatcagat gtaaaatata gtgtttgatt atctgcatga
     7981 ataaaagggc attgttcatc agcaggtgag ttgatgccgg ggcccaggtt ttccgggtta
     8041 ccccatttgc cgtttggctg catccgggaa acataaatat cactgccgcc ataaccgcca
     8101 aatcttctgc ttacaaaata taattcattt ttatccggcg aaagacaggg ttgtgattcc
     8161 cattgatcag aattgatagt gccgccgaga ttcatcgctt cactccagcc attttctgtt
     8221 ttgtaggaga tataaagatc acagccgccc ataccatcgg ggcggtaaca gccggtgaag
     8281 acaagccatt gcccgtcttg tgaaatattt tgagctcctt cactttgctc tgtatttaca
     8341 ttgccctcca ttggctttgc tactccccaa ttaccggatg gggttttact actggtaaaa
     8401 aaatcttcat tgcgtccgtt cagtttcctg gtaaaagcaa gttggttgcc atcaattgtt
     8461 aaacagggaa aatattccga ttcatttgaa ttcacactat cacccatgtt ttgcggagca
     8521 aatacataat tcttttgctg gttattgctg gcatattcca cagcaaattc ataagaacgt
     8581 ttccgcttat acgcattctc cagtgtgaca ggatttttgg gtggtttttt tttcagcagt
     8641 tcattcactg ctgccaatgc tctttcgaat tcacctagac cggcgagatt gatggaataa
     8701 ggaagtttat cctgtaatgt gtaaccggaa tcaatagcaa atgctttttc ataatacccg
     8761 atggctgatt tataattctt cagtatgcca tagatattcc ccagggcaag gtaggcatcc
     8821 acatatttag tatctgtttt aacggcttgt aacaaaagcc cgatagcatt gggatagttg
     8881 ccatcctgtg ctctttcgta agcctggtca aaaaattgaa ccgccttctt atttactttt
     8941 tcaggatcat aaggttgtga aaaagagtat tgaaaaagtg agcagcatgc aaaaaataac
     9001 aacaatctct tcattgatat aagattagga tttctataac gaagctaaac taattttatt
     9061 ccgttgggaa gaggaaaatg ttaaatgaaa agttgcttac ggtacattaa tatacttatg
     9121 tatttgcagg ctcagttccc acgcaggatg ggctttgatg taatctgtta tcaaaggaat
     9181 catttgtgct gctttatccc attcaggttg caggtatagt ttgcaggatg gagacaccag
     9241 tgctgcatat ttttcagccc attcaagatc tgatttgttg aaaataacaa ccttcagttc
     9301 attggccctg ggtaaaattt ccggcagcgg tgctttaaat ttttttggag aaagacagat
     9361 ccagtcccaa ctgccactta aaggatgtgc acccgaagtt tcaatgtttg tttgcaatcc
     9421 ggctgctttt aattgatttg tcagttcatc taggttgtgc atcaaaggct cacctccggt
     9481 aatgactact aattgcgccg gtgttttttt aattgctgca acaatatctt ttatcgcaaa
     9541 ttttggatgg ttctctgcgt tccagctatc ttttacatca caccatacac aaccaacatc
     9601 acagccaccc aggcgaatga aataagctgc cctgccctgg tgaaaacctt ctccctgcag
     9661 ggtatagaaa tgctccatta cgggaagcat tttagtttct ttcaatactt cattcatgtt
     9721 aatccaatca tttattaaat gaaaacagcg gtttaattct tttgcataca ggagcggaac
     9781 gtatttttca tcagcgctgc tattgtcata gggcctacac caccgggaac aggtgttatc
     9841 catgcacatt tgggggccac ggtttcaaag ttgacatcac ctttaagctt gaaaccactt
     9901 ttcttagttg cgtcttccat tcttgtaata cccacatcaa ttacaatcgc cccttcttta
     9961 accatatcgg ctgttacaaa ctcaggtttg cccaatgcag caacgataat atctgcctgc
    10021 aggcaaaaat cttttaaatt ttttgtttgg gaatgacaga gggtaaccgt gcaattgccc
    10081 gggtttgtat tgctgcttaa taaaatactc atcggcctgc caacaatatt actgcgtccc
    10141 accactacag catgtttccc tttggtttcg attttaaaat gttccagtaa taacataatg
    10201 ccatgtggcg tggccggtac aaaagtggga agtccctgta ccatattacc tacattaata
    10261 gggtgaaaac catctacatc tttatccgga tcgatagcat tgatcacttc ttcgtcggag
    10321 atatttttgg gtaatggcag ttgtaccagt attccatcca catccggatc cgtattcaat
    10381 tcctttatct tatcgagaag ttttgtttcg ctgattgtat cttcaaaatg gataagtgtt
    10441 gatttaaagc ccacctcttc acaagctttt actttagcag caacataggt ggcacttgcc
    10501 ccgctatcgc ctactaatat ggccgccagg tgtggtatct ttttcccttc ggcggttaat
    10561 tgcgccactt caattttcag ttcgtctttg attgcctgcg aggctttttt cccatctaaa
    10621 atttgcatgg tgcaaaggta cgtgttgtgg tgtacttatt ctcggggagt gaaaaatcag
    10681 tataattttt ttgaaacaat ttttcatccc tgtaacttac aagtaaaacc ttatcgcttg
    10741 tatagaaaat tcagtttatt actgcttatt gtttttagta tatcaaaaat gccggaagcg
    10801 caaacgttgc ggcgtccgca gactgcaggc tacactggtc ttggagcata cagcctcagt
    10861 catacagata ttttttcttt tacggctaac caggcatcat tagcgcagtt aaaaaatgca
    10921 gctgttggag tgtatggcga aagacgcttt ttactcagtg agttaaataa ctatacagct
    10981 acagccggcc tcattactac ctccggaaat tttgggttga aagctgccta ctccgggttc
    11041 agtgattata acgaaacgca gctcggtttg gcatacggga gaaagctggg tagtaaaata
    11101 gatgtgggtg ctcagtttaa ttataatggt atccgtattg cagaatatgg taatgcggct
    11161 gccctcagtt ttgaaatagg agtcattatg catttaacag ataaactcca taccggtata
    11221 catgtgaata atcctgtagg aggaaaattt gggaaagatc agcaggaaaa actgtcatct
    11281 gtatatgctg ttggttttgg atatgaagca tcagaaaagt tctttgtcag tgcagagata
    11341 gaaaaagaag aagaccagcc ggttaatgta aatgcaggta tacagtataa atttattccg
    11401 caactactgg tcagggcagg cctctctacc gctacatcat cagcctgggc cgggctggga
    11461 ttgtcaataa aatcattccg gctggatgtt acgactagct ggcatccgca gttgggtgtt
    11521 acacccgggt tattggtttt attcaacttt aataaaaagg aagattgaga agtgcagtct
    11581 taatattatt gacaattctc ctttcgctgt actcttatgc ccaggaaatt cctgtaaata
    11641 cggaacaaca attagaaaac ctggctgata cagaccaggc agaaacagag gatgattcat
    11701 acctgcagga gcttgaatat ttcagaaaga acccacttaa tttaaataat gctgaggcag
    11761 aagagttgaa gctattaaga atattaactg atctgcaaat agcgaacctg gtagcctatc
    11821 gcaaattatt tggaaaactt ataagtgtat atgaattaca ggcggtacct gcatgggata
    11881 ttataacgat cagaaaatta ctgccgttta taactgtctc atcatcttta tcattaccag
    11941 aagcgggtaa aagattcaaa gacggagaac atagtttatt gctccggtta tcacaagtat
    12001 tggaaagatc aaagggattt gataatgcaa taaccggtac aaaatattta ggaagcccgc
    12061 agcgattgtt tctgcgttac cggtatacct ataaaaattt attgcagttt ggtctggtcg
    12121 gtgataaaga tgcaggagaa cagttcttaa aaggagcgca gcgatatggc tttgattttt
    12181 attcttttca tcttttcgcc cggaaaatcg gggtgataca ggcattggcc attggcgatt
    12241 ttacagtcaa tatgggtcag ggtcttatac aatggcaaag ccttgccttt aaaaaaagtg
    12301 tggatgtaat gggagtgaag cgacaggctg cagtgctgcg accttatagt tctgccgggg
    12361 aatttaatta tcatcgcggc gctggcatta caatacgaaa ggggagtttt gaaaccaccg
    12421 tatttgcttc actacgaaaa gtgagtgcca attttgtagc tgatactgtc aacaacgaag
    12481 agtatatttc ttcttttctg acttcaggat atcaccgtac caatagtgaa ataacagaca
    12541 ggaacaggat tacccaaaca gcatttggtg gtaatattat ataccggggg aatcgctggc
    12601 atctcggtgt taatagtatt tattataatt tttctttccc ggtacaaaaa agagatgagc
    12661 catataatct ttatgccatt agtggaaaga actggtacaa cttcagtatc gactatagtt
    12721 acacgtacaa gaaccttcat ttgtttgggg aagcagcttc ggataaaaat ttcaacaaag
    12781 cttttatcaa tggactactg atcagtgtgg atcccaatgt cgatctgtca tttgttcaac
    12841 gaacgatcag tgccgcttac cagtctgtca acgggaatgc atttacagaa aatacatatc
    12901 cgactaatga atcaggtttt tacgccggta tcagcatccg tccgggtatt ggatggcgta
    12961 tagatgcata tagtgatatt ttcaaattcc cctggctcaa atacctggtc gatgctcctg
    13021 gcagtggtaa agatttcctt gcccaactta cctatactcc caaccggcag gcagagatgt
    13081 atacaagatt caggaatgaa actaaaaagg gaaatcaacc cggcaacctg actacaacta
    13141 attttttggt ttcattaccc cggcaaagct ggcggacaca ggttaattat aagcttaata
    13201 ccaccattag tctgcgaaac cgggtggagc ttatgtggtt tgacagaaaa ggggaaaaca
    13261 gcgaaaaagg gtttttaacc tttgttgatg ttgtatataa gcctatgctg aagccgttct
    13321 caggtataat ccggctacaa tattttgaga cagatgatta taattcaaga atttatgctt
    13381 acgaaaatga tgttttatac agctattcaa tacctgcttt ctttgataaa ggttttcgtt
    13441 attatataag cttaaattat gattggggaa aaaagctcac attttggctt cgctgggccc
    13501 aaactattta caaaaacagg gacacagtcg gttcgggtct ggatgaaata cccggaaatc
    13561 ggcgatctga agtgaaattc caggtcaggg ttttatttta gtactaatgc tcgttcaata
    13621 ttttttgctg atttttaact aattgttaaa aaaaaaatta acttgccgca gcattttagg
    13681 acgcatctat gtcttaaata acatccttac ccctgaaaat ttaacggttt tccctgataa
    13741 tcaatgcttt taaattttaa aaattaacgt taacatgaga aaaatcgcat cactgtttac
    13801 gatgctaatg ctgttttctg ctttagcatt tggtcaaaac cggacgatta ccggtactgt
    13861 gaccgatgaa actggtgctc cactacccgg agcttccgtt agaatcgaag gaacccgtac
    13921 aggtgtggct gctgacaata atggtcagtt ccgcattctg gctaaaacgg gggatgtact
    13981 cgttgtttca ggcgctggtc ttgaaactac aactttaacg gtaggagccg gaaacacggt
    14041 atctattact gtaaaccgac ttgttttaac tggtactgag gtggtggtaa cggccatggg
    14101 ccaaacccgc caggccaaag aattaggtta tgctactacg aaagttaaag cggccgaatt
    14161 gacccaggca aaagcagtta acctgcaaaa tggtttgact ggtaaagttt ctggtttgaa
    14221 cattcaaact gtaaacaacg gcgttttcgc tgataccagg atcacacttc gtggtatccg
    14281 ttctttgact ggtaataacc agccgatgct gattttggac ggtgtgccca ttgctttggg
    14341 ttacctcagt tcaattaacc ccaatgacat cgtagatgta acaatcctta aatctgcttc
    14401 cgctactgct atttatggtc ctgatggtgc taacggtgcc ctggtggtta ctaccagaaa
    14461 aggtagtaag accaaaccac aggttagtgt aggacatact gtacagatag agagagtatc
    14521 ctatatgcca gaactgcaaa accaatttgg ttcaggctct tcaatagatc ctttcggtta
    14581 tggtgtgtat gaccccattg agaaccagtg ttatggtgat gaatttgatg gttctatgcg
    14641 ccagataggc cgtgatggtc ctaatggtga gcagtacctt gttgaatata ttgcccgccc
    14701 tgaagagaaa agaaaattct ggaataccgg tatcactaac cagacagatg tatcattctc
    14761 cactggtgat ttctacctga gtgctcagaa tgtactgatc cagggtatca tgccaaaaga
    14821 cgttaaccgc agggtttccg ttcacatggc tggtaataaa gagtataaca ggttcaaagc
    14881 ttcttatagc atcaactata caaaaggtaa ctatgatgtt actgccggct ccagctttgg
    14941 taacggtcgt gactatactg tatattggaa cctgatcaac actcctcagc aaattccgat
    15001 cacaaggttt aaagactgga gaaataacta ttgggcaaat ccaaatggat atttcaacga
    15061 ttattatttt aacccttact tcatgatcga taatttcaga agtgtgggaa gaacagaaga
    15121 tatcttcgga aactttgaat tgaacttcaa agctaccaac tggttaacat tcacttaccg
    15181 tttaggtgct accatctcta atggtaatgc caagagcagg gcagaagcat tcacttatag
    15241 tgatttcgct aaagcttcag gaaaatcaaa tgctcaatcc ggtgatgtgg ttgcccaggt
    15301 gggtgatggt atctctacct caagccgtat caactctgag ttttttgcaa ctatccgcaa
    15361 aaaggcagga aattttgatt ttaatgcctt attgggtcag tcattccgtg aggataactc
    15421 aaaaaatata aatgtttcca gtaataacct ggctttcccg acccttttca atgtaattgg
    15481 tcgtaaaggt gagcctggtg cttctgaaag taatttcaaa tcaagattgc agcgtttctt
    15541 tggtaaattg tcagttggtt acaacaactg ggcatttgcc gaggttactg gcagctatga
    15601 tatgaacagc cgtcttgctt attattatga ttttgatttc aaagcaataa gcttcttcta
    15661 tcccggagcc agcctttctg tattgttaag tgaggccatt cctgcaatca agaacagtaa
    15721 ggttatttct tatttcaaga ttcgtgcagc taaatctaaa accggtaacg taaacctggg
    15781 tgttcaagcc cttgaaaata cttacggatt aggtggtggc ttcccatatg gttcattggt
    15841 gggctatact tctggtaacg ttttgcgttt gccaaaatac actcctgagt tcgttaagaa
    15901 taacgagatc ggttttgaat taggtttatt gaagaaccgt atcaacctgg aggctactta
    15961 ttatacacag aacaactctg accagatcgt tcaggtacag tattcaggtg ccactggttt
    16021 taccagtgct ttgttgaatg cagcttcctt tactaatgat ggttttgagt ttgacctgag
    16081 attaactcca ttggtgaaga ttcgcgacgt atcaattgat ttcaaagcaa actatactta
    16141 ccagactaac gaagttaccc agatcatcga aggtgttgac gagttaggta tcggtaacgc
    16201 taactatgta attgttggca agccagctta tactttccag ttaactgact atgtaagaga
    16261 tgaccagggt cgtgtaattg ttaacagttc aactggttta ccaaccgttg accccgttat
    16321 caagcctttt ggtcacacac agccaaacca tttaataggt ttgaacctga atattagctg
    16381 gaaagacctt acgttcagcg cagttgctga ttatcgtggt ggtgcccaga tctatactgg
    16441 taacctcggt acaggtatgg acttctctgg tatctcaaaa agaagtgctc agaattcccg
    16501 tcagcctttc atcatgccga attcatctta ctgggatggt tctaaatatg tagacaatac
    16561 aagcatctat atgactggcg gttataattt ctggtctcag gcaactaacc agaatgcgaa
    16621 cactaactac ctggttagcg gcgacttctg gaagatcagg gagatgagct taagctataa
    16681 tattcctgcc aaagtatttg gatttactaa gaatgcaatt aaaggtgcca ctttcacatt
    16741 gtccggaaga aacctcttta tgttcctgcc taagacaaat gaatggacag atcctgagtt
    16801 tgctaataca acaggaaatg cacaaggggt gagtggtttg gataatacac ctcctacaag
    16861 aatttttggt gcaaccttaa accttaattt ataagaattg taataaatta ttaagatatg
    16921 aaactgaaaa aattaatata tggtgtccct ctcgcagtaa tattgattgc tgtgggatct
    16981 ggttgccgga agagccagtt taacatcaac cagaatacaa ataaccctac ggacagtacg
    17041 gtaagctatg atgtagtatt acctgcagcc cttcatgcca ctggtggtat cgtgggtaca
    17101 tcctggggtc ctattcagaa ctggatgggc ttttgggcaa ggtctggtac gtatgcgcca
    17161 aacgtactcg aggaaaccta cgagatcact actggtttcg ggaatggtat ctggaataac
    17221 tgttacgata ataactatga ttaccagata gtacagaata aagcaagtgt tgccggtgca
    17281 ggtttttatg cagggatagc ccgtatcatg aaagctcata attt
//
LOCUS       DAORTI010000071        17063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3064_length_17063_cov_6.128175, whole genome shotgun sequence.
ACCESSION   DAORTI010000071 DAORTI010000000
VERSION     DAORTI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17063
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_17063_cov_6.128175"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLO70_09995"
     CDS             complement(<1..195)
                     /locus_tag="PLO70_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQZ74841.1"
                     /translation="MGDFQVKKQSKKYDAVIVGSGAGGGMAAYMLAKAGLKVCIIEAG
                     YMYDPQKNITQLKNPWDSPRR"
     gene            319..2295
                     /gene="pulA"
                     /locus_tag="PLO70_10000"
     CDS             319..2295
                     /gene="pulA"
                     /locus_tag="PLO70_10000"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HQZ74842.1"
                     /translation="MKRFIMLISFLAHLFHGKAQRTDYSKYPVYKGNDLGLTYTPKWS
                     LFKIWAPTAAAAEIINYSDGTGGKEINRAQMKKEENGAWSVLAKTDMKGTFYTFRVNI
                     NGKWSEEVPDPYAKATGVNGKRAAVVDLKETNPLGWEKDTSPGFSNKTDAIIYELHVR
                     DASISATSGIKHKGKFLGLTETGTKNPAGLATGLDHLKELGITHVHLLPVYDYNSVDE
                     TKPDTKYNWGYDPLNYNVPEGSYSTNPYDGLTRIKEFKQLVKTFHENGLRVVMDVVYN
                     HTALTATSNFNQLVPGYYYRQTKDGKFSDATACGNETASERPMFRKFMLESMKYWVEE
                     YHIDGFRVDLMGVHDIETMNLVSKELHKIKPDILIYGEGWTAGASPLPDSMRALKANA
                     HKLDRIAVFSDDIRDGIKGSVFEHEDKGFASGKPTMEETIKFGIVASCKHPQVDYSKV
                     NYSKAAYAKVPSQTVSYAECHDNHVLWDKLAISAKEATEAERIEMHKLALSIVLTSQG
                     ISFIHAGTEFLRSKKGVENSYESSDSINAIDWGLKSGNKEVFEYIKALIKLRKDHPAF
                     RMMEADKIKKFISFDNEMPAGIISYSINGWANKDKWKTIRVIFNGTGKVQKLSTSGTW
                     NYAIRNNKFSADENEYSVPVLELAPYSCTVLYQR"
     gene            complement(2296..3330)
                     /locus_tag="PLO70_10005"
     CDS             complement(2296..3330)
                     /locus_tag="PLO70_10005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF023384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQZ74843.1"
                     /translation="MKKILLSSIVTISSLFAYAADTDSTIHFNLPDTVKAVQFLAEIR
                     IDDVNSKKEVQAGIRTDLVKLSLEADKNEREIVFEFPGSSKIVATGINVETEDDELEW
                     DYNWVTGENYKLLLSIAQDSAADFLLYSGYIWLPRESKWKLIGTCKIEGQWGSIKKPE
                     IFYSAGLRKKQPVIRVHTGEAWIQRNNGSWKNLNEGNLKSPVVNVTSHIDSLAQHEKE
                     IAIIKEAIASGKTDAVNEHNSIYYVMLKEGTGKQVSVTDTVVIHYKGSLFSDGSMFDQ
                     SKDKTSTFPLNRLIKGWQIGLPLCKTGSKIKLVIPSAHAYSTRTRAAKIPPNSILVFE
                     IEVFDTKPKL"
     gene            complement(3343..4446)
                     /locus_tag="PLO70_10010"
     CDS             complement(3343..4446)
                     /locus_tag="PLO70_10010"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HQZ74844.1"
                     /translation="MKKNFLFIILIVAGTGISAQIKDMFIAKKDSSGFVLTLKGASHL
                     ATLPLKCIQQEFPNKTSHTSNSDSDHVLLPRQLHPSFYGCFDWHSSVHGHWMLVKLLK
                     LFPGIPEATAIRKGLNKTITVENILDEVKYFDMPLAKSWERTYGWAWLLKLDEELMSW
                     DDMDGKKWHTALQPLTKKVLEIWANFLPKQTYPNRTGVHPNTAFGLVFALDYARAAKH
                     AAFEKAIIESAIKIYGKDKNAPALWEPNGADFLSPSLEEADLMRRVLSPGEFTIWRKG
                     FLNAKSLQHLSVLPVVSDRNDLQIVHLDGLSFSRSWCMQELTSTLSPKDPLKKLLQRS
                     AMNHLATALPHVVSGSYGGEHWLASFAVYALTK"
     gene            complement(4546..4890)
                     /locus_tag="PLO70_10015"
     CDS             complement(4546..4890)
                     /locus_tag="PLO70_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQZ74845.1"
                     /translation="MKLFVAGLPYDMDDAELEEFFEKFGTVTSVKVAMDRETGKSKGF
                     GFVEMPNSTEAKEAIEGLNDLPIGRKTLVVKEAEEKQGGGGGGYRGNSGGSGGGSNFN
                     RYGNNRPPRNRY"
     gene            5077..5478
                     /locus_tag="PLO70_10020"
     CDS             5077..5478
                     /locus_tag="PLO70_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74846.1"
                     /translation="MKLQVSRSKTIKELQVEFATCFPNLKIEFFASTHKPEEGSFGKL
                     QFPRTMKLGELSPMLNEGEIAVSPGMRTGHLEQVFHAEFGLNAQVFRNMNGTWIQSTN
                     SDDLSLLEQNEIAQASIPKKSSMKNVEERDY"
     gene            complement(5511..5759)
                     /locus_tag="PLO70_10025"
     CDS             complement(5511..5759)
                     /locus_tag="PLO70_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74847.1"
                     /translation="MARSHHRKKHKEQLRQFKHSQDMAENSGAKSKAANIIMIGGALA
                     GFAIGYFASGGVILWMTVGLLAGAAAGYYVGKKIDEER"
     gene            5902..7785
                     /locus_tag="PLO70_10030"
     CDS             5902..7785
                     /locus_tag="PLO70_10030"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQZ74848.1"
                     /translation="MRMFLLSILIFALVLTSFSQQTQKPVLHGKNWMAITGKPLAATA
                     GAITFQKGGNAVDAACAMLAATCTMWDVLSWGGETQVLLYNPKTKKVIAINAMGVAPT
                     GATPEFFKGKGYNFPPEYGPLAATTPGTPGGLCYMLAEYGTMSLKEVLAPAMQLAAGY
                     PIEAQTANSMERGKARIKEWPYSKKVFLPHTGEKREAPEAGEIFIQKDLLQTLTKMVE
                     AEQDALKKKKSRKEAIMAAYNRFYKGDIAKEFVRGCQEQGGLITMEDLAKWKPIEEEP
                     MKVNYKGIDVYKLQQWTQGPVLLQSLNILENFDLKSMGYNTSKYIHTIYQTMNLAFAD
                     RDFYYGDPYFPPAEPMKGLLSKEYAKQRASFINFEKNDALIGPGDPYPYEGKTNPYLQ
                     LMKQRGFATDTTKRNFMPSHDIRNSASVDHEYMDRLWRGTTSVEAADKEGWVVSITPS
                     GGWLPACIAGNTGIGMSQRMQSFVLDSALNPFNVVAPGKRPRVTLTPTLALRDGKPYL
                     SFAVQGGDTQDQNLLQFFLNVVEFGMNVQQAAEAANINSNQLWLSLGGSKTDDRKPKP
                     GNILLHDKTPEKVRNELKQMGYTLSFDDRTSGPINAIYFDWKHGSFWGGSSNHGEDYG
                     IGW"
     gene            7814..7984
                     /locus_tag="PLO70_10035"
     CDS             7814..7984
                     /locus_tag="PLO70_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74849.1"
                     /translation="MIWSYLLIGGGIVVLLLLLALQNRKDKRKFIDTLNQDYKKHEKH
                     KDDEDPDDLKAG"
     gene            8082..9263
                     /locus_tag="PLO70_10040"
     CDS             8082..9263
                     /locus_tag="PLO70_10040"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQZ74850.1"
                     /translation="MKEVVIVSAVRTPMGSFGGSLKSLSATQLGATAIKGALQKAGIK
                     PEQVNDVLMGCVIQANLGQAPARQAAKFAGLPNEVNCTTVNKVCASGMKAIALAAQSI
                     ALGDADIVIAGGMESMSNVPFYADSVRWGNKYGNASLIDGLAKDGLTDVYDGKAMGNA
                     AELCASTCGISREEQDAFAIESYKRSQSAWEKGLFANEIVPVEIPQRKGDPIVFAKDE
                     EPFNVKFDKIPSLNPAFIKEGTVTAANASTMNDGAAALVLMSADKAKELGLKPIAKIR
                     SFADAEQAPEWFTTTPSVAVPKAVSKAGLKMEDIAYWELNEAFAVVGIENSKRMKLDP
                     LKVNVHGGAVSLGHPLGCSGARIIVTLINVLKANNAKYGAAGICNGGGGASAMVIENI
                     E"
     gene            9349..9873
                     /locus_tag="PLO70_10045"
     CDS             9349..9873
                     /locus_tag="PLO70_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74851.1"
                     /translation="MITIRMATPADAALIAKLSRQTFYETFADSNTASDMDKFMNEQF
                     NNEVLMKEVETPGAIFFLAYDDEEPVGYVRLRDGEHHPEFKGASSIEIARIYALKKSI
                     GKGIGRELMHKCIDIAREMNRDIIWLGVWEKNERAIDFYQKWGFEKFAEHEFVLGNDV
                     QTDWLMCKHLFKNK"
     gene            10065..10994
                     /locus_tag="PLO70_10050"
     CDS             10065..10994
                     /locus_tag="PLO70_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74852.1"
                     /translation="MKPRILSLVISILSISILFTACKKEGSVSQDYSAETAAHSDDQS
                     RFSTEVDAVANDANLALEVTPAFSGRGGEIQALICDADITVDTLANPRTITITYNGTN
                     CLGNRTRTGVIVLSMAQGVRWKNAGAALSVSYQNFKVTRVSDNKSITINGSQTYTNVS
                     GGLLLNLPVLGTITHTITSSNMSVTFDNGSQRTWQVARQRVFTYNNGVVITVTGTHTD
                     GAVTGIAEWGTNRFGRSFITAITQPLVLKQDCSFRVGSGKVVHTTPMYNIAATFGLDA
                     NGNATSCPGTGYYYMKLEWTGPGGNTQTAVLPY"
     gene            11091..12332
                     /gene="hutI"
                     /locus_tag="PLO70_10055"
     CDS             11091..12332
                     /gene="hutI"
                     /locus_tag="PLO70_10055"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQZ74853.1"
                     /translation="MATTLIINIKLLVNTPGENRLLRGKELSKLPCIANAYLIIEDGI
                     ITGIGDSSEIQNPKSKFKNIIDATGQYVLPAWCDSHTHLVFAASREEEFVDKIKGLSY
                     ADIAAKGGGILNSAKKLQTATETELFTLAWKRLEEVSKLGTGAIEIKSGYGLSVDDEL
                     KMLRVIKKLKEKSVLSIKSTFLGAHTYPLEYKDNHQGYIDLIINEMLPVIAKEKLADY
                     IDVFCETGFFSPEETEIICKAGMRSGLKPKIHANQLNLSGGTQTGVKLNAVSVDHLET
                     MDDEAITTLAGSNTIGTLLPTAAFFLRMPFQPARKLIDAGCAIALASDYNPGSSPSGN
                     MNLVTAMSCIQMKMLPEEAINAATINGAYAMELQDEVGSIMIGKKANLIFTKSIPSLA
                     YLPYAFGSNLIDKVMISGEFC"
     gene            12342..12731
                     /locus_tag="PLO70_10060"
     CDS             12342..12731
                     /locus_tag="PLO70_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74854.1"
                     /translation="MLKLILWVLSSLIATVIFSQNSIPDCSFFKKGDFIYKDSSGVTW
                     DIKRTGKYQTERNKQTGLLIKHKITWVSNCEYKLTQIWSNNKDRRKYNRSWIIYKILR
                     VTDKYYSYSCTCTDGREITGVVVKLID"
     gene            12894..14300
                     /locus_tag="PLO70_10065"
     CDS             12894..14300
                     /locus_tag="PLO70_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ74855.1"
                     /translation="MSLRQFISSRKFLRSFDIIKENVSLKWKEVAELFNLHEDVFENP
                     RHIRLKKPVSVILIGAGHRGGIYADYAVENPDEMKIVSVSDINVKRRNTIKKLHNISD
                     TYCYSNWQDVFKVPKFADAVIIATPDQLHTQPCLAALEAGYDVLLEKPIAPTEEECRL
                     IYNKAQASNNIVGVCHVLRYSPYFRELKEVLDAGLIGNIISLQHMEPIEQIHMSHSYV
                     RGKWRNSKSATPILLAKSSHDTDIIRWLVNSPAKDVHCFGNLKWFTNINAPEGSTERC
                     TDGCKVESSCPYSALQIYLRDKKRLYVFDMPENKSEWDDFILEKLKTTDYGRCVYRMD
                     NDQPDHLTVNMLFENGVTAAFSMEAHVSYEGRRTRIMGSKGDIVGNMESFIMTDFKTR
                     KQTSWKMKTDAHGGGDHRLVKDWIQAVAQNDKSLLSSSIDVSIESHLMAFAAEKSRLN
                     RTIEEVKLISNEYLIENG"
     gene            complement(14325..15548)
                     /locus_tag="PLO70_10070"
     CDS             complement(14325..15548)
                     /locus_tag="PLO70_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQZ74856.1"
                     /translation="MKEIGAYGYIIAICLLVILSYLFNLISRRTGIPSVLLLLLTGIG
                     VRETLVFNNISVEVSPQMVEIFGILGLIMIILEAGLDLQLGKKKIKLIRNAFFSGLII
                     LVLSAIAISSFLYFYLKEDFILCLIYALPLSVVSSAIVIPSISHLDEHKKEFLVYETS
                     FSDILGIIFFNYLIAGNLLKGSNITWFFGSLIISIVLSIVLSFLMLFMLTRITTKVRF
                     FLVFAVLIFLYAGGKMLHLPSLFIILLFGLIVSNWQLTIFNRLYKWVSIQQVDDVSHL
                     LHSLTAESSFLIRTFFFFIFGLTIDIRLIMDKDVILAGSAIVILLLFIRFIYLRFFLR
                     SHIVPEVFFMPRGLITILLFYSIPQLYKLSKFNEGILFFVILITSIIMMIGSIAYGKP
                     KLQAINDEIPLSDEP"
     gene            15668..16987
                     /locus_tag="PLO70_10075"
     CDS             15668..16987
                     /locus_tag="PLO70_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HQZ74857.1"
                     /translation="MYSKQAYEIFLSATMAVQPQLLLPEYIYSKNGFLHLTDQEFNIR
                     DINRFIVIAAGKAAPGMANVTEKQVGILVSEGYCITKYGHSLPLEYFHTIEAAHPVPD
                     QNSIKAVEIVLQAVNGLNVDDIVLVLLSGGASSLLADIPEGCTLEETKNIFKMLINSG
                     ASIHEINIVRKHLSKIKGGQLAKAVYPAKLFTLIISDVVGDHLESIASGPTVSDSSTF
                     EDAYRILQKYNIWTSTAESVRKYILKGVNQTIEDTPKPGAHFFYNTTTKIIGSNNLAL
                     NMAKQKAEQFGYHSFILTDNLTGNADTEARKFVHYLQHYSGEKPVCILMGGETTLKVT
                     SPGKGGRNQHFVLSAINELLKNSISGTDHDITILSAGTDGTDGPTDAAGALFNNKSLP
                     AFMGMNEMIQEHLDRFDSYSYFYKMGGLVITGPTQTNVMDIVVALIH"
BASE COUNT         5517 a         3149 c         3546 g         4851 t
ORIGIN      
        1 ccggcggggt gagtcccacg gattttttaa ttgagtgata tttttttgcg ggtcgtacat
       61 gtaacctgct tcaatgatgc aaacttttaa tccggccttc gccagcatat aagcagccat
      121 cccgccaccg gctcctgagc ctacgataac ggcatcatat tttttggatt gtttttttac
      181 ctggaagtct cccataaagc aagaatgaaa taaataattg aaacaacaat ttacaggttt
      241 gataaattga ttccgaaatt atttattcaa acttctgcac attatcatta tgtatcttta
      301 aaaaaaaata gtagttcaat gaagcgtttt attatgttga taagttttct cgcacattta
      361 tttcatggca aagcacaacg tactgattat agtaaatatc ccgtgtataa aggaaatgat
      421 cttggactaa catatactcc taaatggtct ttatttaaga tttgggcacc aactgccgct
      481 gctgctgaaa taataaacta tagtgatggt acggggggaa aagaaattaa ccgggcccaa
      541 atgaagaaag aggagaacgg agcctggtca gtgttggcta aaactgacat gaaaggtact
      601 ttttatacgt tcagggtaaa catcaatggg aagtggtcag aagaggttcc cgatccttat
      661 gcaaaagcga caggagtaaa cggtaaaaga gctgcagtgg ttgatctgaa agaaaccaat
      721 ccattggggt gggaaaaaga taccagcccg ggattcagca ataagacaga tgctatcatt
      781 tatgaactgc atgtaagaga tgccagtatc tctgcaactt caggtataaa acataaagga
      841 aaatttttgg gattgactga aaccggcaca aagaacccgg ccggtttagc taccggtctt
      901 gatcatttaa aagaattagg tataacacat gttcacctgc ttccggttta tgattataat
      961 tcggtggatg agacaaaacc ggataccaaa tataactggg gatatgatcc gttgaattat
     1021 aatgtgccag agggttctta ttccactaat ccttacgatg gtttaacacg gataaaagaa
     1081 ttcaagcaac tggtaaaaac atttcatgaa aacggattaa gagtcgttat ggatgttgta
     1141 tacaaccata ctgctttaac tgcgacttct aattttaacc agttagttcc cggctattac
     1201 taccgtcaaa caaaagacgg aaaattctct gatgccaccg cttgtggtaa tgaaacggcc
     1261 agtgaaagac cgatgttcag aaagttcatg ctggaatcaa tgaaatattg ggtagaggaa
     1321 tatcatattg atggcttccg ggtagacctg atgggagtac atgatataga aacgatgaac
     1381 ctggtatcaa aagaactgca taaaataaaa cctgacatat tgatttatgg cgaagggtgg
     1441 acggcaggag cttcaccatt gcctgattca atgagagctt taaaagccaa tgcacataaa
     1501 ttagacagga tagctgtttt cagtgacgat ataagggatg ggataaaagg tagtgtgttt
     1561 gaacatgagg acaaaggttt tgccagcggt aagccaacaa tggaagaaac aataaaattt
     1621 gggattgtgg cctcctgcaa gcatccacag gtggattaca gtaaagtgaa ttattcaaaa
     1681 gctgcttatg caaaggtacc ttcccaaaca gttagctatg ctgagtgtca tgacaatcat
     1741 gtactctggg ataaactggc gatctcagcc aaagaggcta cagaagcaga aagaattgaa
     1801 atgcataaac tggctttatc aattgtactg acctcccagg gcatctcttt tattcatgcg
     1861 ggtacagaat ttctgagaag taaaaagggg gtggaaaatt cttatgaatc atcggatagt
     1921 attaatgcca ttgactgggg attgaaatca ggtaataaag aagtgtttga gtatataaaa
     1981 gccctgataa agttaagaaa agaccatccg gcattcagaa tgatggaagc agataagata
     2041 aaaaaattta taagttttga taatgaaatg ccagctggta tcatctctta ttcgataaat
     2101 gggtgggcca ataaggataa atggaaaacg atccgggtta tatttaacgg aaccggaaaa
     2161 gtacaaaagt tatcaacctc cggcacctgg aactacgcta tacgaaataa taagttttca
     2221 gctgacgaaa atgaatattc agttccggtg ctggaacttg ccccatacag ctgcactgta
     2281 ctttaccagc gatgattaca attttggttt tgtatcaaaa acttcaattt caaacaccag
     2341 gatactattg ggcggaattt ttgctgccct tgtccgtgtt gaataagcat gtgcagaagg
     2401 aataaccagt tttattttac tacctgtttt gcaaaggggt aacccgatct gccatccttt
     2461 tattaaccgg ttcaggggga acgtagatgt tttgtctttg gactgatcaa acatggatcc
     2521 atcactgaag agcgaacctt tgtaatgaat gaccactgta tcagtaactg aaacctgttt
     2581 acctgttccc tctttgagca tcacgtagta aatactgtta tgttcattta ctgcatccgt
     2641 ttttcctgat gcaattgctt ctttaatgat ggcaatttct ttttcatgtt gggcaagact
     2701 gtcaatatga gaggttacat taactaccgg actttttagg tttccttcat tcaggttttt
     2761 ccaggatcca ttgtttcgtt gaatccaggc ctcacctgta tgtacccgga tcacgggttg
     2821 tttttttcta agacctgctg aataaaatat ttccggcttt ttaatagaac cccattgtcc
     2881 ttctatttta caggtcccaa taagtttcca cttactttcc ctgggaagcc atatataacc
     2941 tgaataaagt aaaaagtcag cagcagagtc ctgtgcaata gaaagaagta gtttataatt
     3001 ttcaccagtt acccaattat agtcccactc cagctcatca tcctctgttt caacatttat
     3061 ccctgttgct acaattttgg aagatcccgg aaattcaaat actatctctc tttcattttt
     3121 atcagcctcc agtgatagtt ttacaagatc tgttctaata ccggcctgaa cttctttttt
     3181 tgaattgaca tcatcgatcc tgatctcggc aaggaactga accgctttca ctgtatcagg
     3241 aagattaaaa tgtatggtcg aatcggtatc agccgcatat gcaaaaagag aactaattgt
     3301 cactatgctg gataaaagga tttttttcat gtttacagtt tattattttg tgagtgcata
     3361 tacagcaaaa ctggccaacc agtgttcacc gccataacta ccactcacta catgtggtaa
     3421 ggcagttgcg aggtgattca ttgccgatcg ttgtaatagc ttctttaacg gatctttagg
     3481 cgaaagggtg gaagttagct cctgcataca ccaactacgg ctgaaggaaa gtccgtccag
     3541 atgaacaatc tgcagatcat tgcgatccga aacaacaggt aataccgaaa gatgctgcag
     3601 gctctttgca tttaaaaaac cttttctcca tatcgtaaat tctccgggag ataaaaccct
     3661 tctcatcagg tcagcttctt ctaaactcgg agaaaggaaa tcagcgccat taggttccca
     3721 gagggctggt gcatttttat ctttcccgta aatcttaata gcagattcaa taatagcttt
     3781 ttcaaaagct gcatgttttg cagcccttgc ataatctaat gcgaaaacaa gcccgaaagc
     3841 ggtattaggg tgcaccccgg ttcggtttgg ataggtttgc ttgggcagga agttggccca
     3901 gatttccagt actttttttg taaggggctg taatgctgta tgccattttt taccgtccat
     3961 atcatcccag ctcatcaatt cttcatccag tttgagcagc caggcccatc cataagttcg
     4021 ttcccagctt tttgcaagcg gcatgtcaaa atattttacc tcatccagta tattttcaac
     4081 agtaatagtc ttattgagac ctttcctgat tgcagtagcc tcaggtatcc ctggaaaaag
     4141 ttttaaaagt ttaaccagca tccaatggcc atgtacggat gaatgccagt caaaacaacc
     4201 ataaaaagac gggtgaagct gacggggcaa taatacatgg tcgctatcgg agtttgaagt
     4261 gtggctggtt ttattaggga attcctgctg gatacacttc aatggcaaag tagccagatg
     4321 cgaagcacct tttaaagtaa gcacaaagcc tgagctatcc ttttttgcaa tgaacatgtc
     4381 tttgatctgt gcagaaatcc ctgttcctgc tactatcaga ataatgaaaa ggaaattctt
     4441 tttcatttat caaacataat aaataatgta ctcctgatgt cttggaaatg tatgggaaaa
     4501 acaaaacccg gctgattgcc gggctatacg tttattttta aatgtttaat acctgtttcg
     4561 gggaggtctg ttatttccat accggttgaa attactaccg ccgccgctgc cgccactatt
     4621 tccccggtaa ccaccgccgc cgccaccttg cttttcttcc gcttctttta ctacaagtgt
     4681 ttttcttccg attggcagat cattaagacc ttctattgct tcttttgctt ccgtgctgtt
     4741 aggcatttct acaaaaccaa accctttact tttgcctgtt tccctgtcca tggctacttt
     4801 aacagaagta acggtaccaa atttttcgaa aaattcttct aattctgcat cgtccatatc
     4861 ataaggtaat ccggctacaa aaagcttcat ctgaataaat ttctacaaaa ataagcaaga
     4921 atatgggtat taacagacct aatttccaaa agaattcaac tttcttggag tgattagaat
     4981 cagataaaaa agtaacctgt atcatattcc tccttcataa agtaccagaa cttcaatcaa
     5041 caggatttag tattacttct aactaaaaaa tcagacatga aacttcaggt atcccggtca
     5101 aaaacgatta aagaactgca ggttgaattt gccacctgtt tcccaaacct gaaaatcgaa
     5161 ttttttgcat ctacgcataa accggaggag ggttcattcg ggaaacttca atttccgaga
     5221 acaatgaaat tgggagagtt gagcccgatg ctgaatgagg gagaaattgc tgtttccccg
     5281 ggtatgcgga ctggccacct ggagcaggtt tttcatgcag agtttggtct gaatgcacaa
     5341 gtattcagaa atatgaacgg cacctggatt caaagtacca acagtgatga tttatcctta
     5401 ttggagcaaa atgaaatagc gcaggcttct atacccaaaa aatcttcaat gaaaaatgta
     5461 gaagaaagag attattaaac agcagtggat ttaaagacta ttgattgtac ttatctttct
     5521 tcatcaattt ttttaccgac ataataacct gcagctgctc ctgcaagtaa cccgactgtc
     5581 atccagagaa taacaccccc tgaagcaaaa tatccaatgg caaaacctgc caatgctcca
     5641 ccgatcataa ttatattagc agccttactt tttgctcctg aattttctgc catatcctgg
     5701 ctatgtttaa attggcggag ttgttcttta tgtttttttc tatgatgtga tcttgccatt
     5761 tttattttga aattagtaaa acattttgtt ccaaaaaagt atcctgatca gaatcaggct
     5821 gattattggg ggagtatgac aataaacagt tttaccggat ttttattctt acacttatct
     5881 ttattatcta aaatatatgc catgcgaatg ttccttctat caatactgat ttttgcccta
     5941 gttctgacat cattttcaca acaaacacaa aaaccggtat tacatggaaa aaactggatg
     6001 gccataactg gaaaacctct tgctgccaca gccggcgcta ttacgtttca gaaaggtggt
     6061 aatgctgtag atgctgcctg cgccatgctt gcagcaacct gtaccatgtg ggatgtgtta
     6121 agctggggtg gagaaaccca ggtactactt tataatccta aaacaaaaaa agtaattgct
     6181 attaatgcaa tgggtgttgc acctacaggc gctacacctg aattctttaa aggtaaaggg
     6241 tataattttc ctcctgaata tggccccttg gctgcaacta ctcccggcac acccggtggt
     6301 ttgtgctaca tgctggcaga atatggcacc atgagcttaa aagaagtatt ggctcctgcc
     6361 atgcagttag ctgccggata tcctatagaa gcacaaaccg ctaatagtat ggaaaggggt
     6421 aaagccagaa ttaaagaatg gccctatagc aaaaaagttt ttcttcctca tacaggggaa
     6481 aaaagagagg caccggaagc aggagaaata tttatacaaa aggacctgtt gcaaacactt
     6541 actaaaatgg tggaagcaga acaggatgct ttgaagaaaa agaagagccg taaggaagca
     6601 atcatggcag cttataacag gttctacaaa ggagacattg ccaaagaatt tgttcgtggt
     6661 tgccaggaac agggtggact aataaccatg gaagatcttg ccaaatggaa accgatagaa
     6721 gaagagccca tgaaagtgaa ttataaaggt attgatgttt ataaactgca acaatggaca
     6781 caaggacctg tgttacttca atccttgaac atattagaga attttgactt gaaaagtatg
     6841 gggtacaata ccagtaagta tatccatacc atctaccaga ctatgaacct ggcatttgcc
     6901 gaccgggatt tttattacgg agacccgtat tttccgccgg cagaacccat gaaaggcttg
     6961 ttgagtaagg aatatgcaaa gcaaagagca tcattcatca attttgaaaa aaatgatgca
     7021 ttgataggcc cgggcgaccc ttacccgtat gaaggaaaaa ctaatcccta cctgcaatta
     7081 atgaaacaaa gaggatttgc aactgatacc acaaaaagaa atttcatgcc gtcacatgat
     7141 atcagaaaca gtgcgtcggt tgatcatgaa tatatggaca ggctttggcg tggcaccacc
     7201 agtgtggaag ctgctgacaa ggaaggttgg gtggtatcaa tcacaccaag tggaggctgg
     7261 ttgcctgcat gtattgcagg taacaccgga attggaatga gtcagcgtat gcagagtttt
     7321 gtacttgatt ctgcactaaa tccctttaat gttgtggcac ccggcaaaag gccaagagtc
     7381 acattaacac caaccctggc actcagggat ggcaagcctt atttgtcctt tgccgttcag
     7441 ggaggggata cacaggatca gaacctgctt cagttttttt taaatgtggt ggagttcgga
     7501 atgaatgtgc aacaggcagc tgaagctgca aatatcaata gtaaccagct ttggctttcc
     7561 ttaggaggtt caaaaacaga tgacagaaag cccaaaccag gaaatatatt attgcatgac
     7621 aaaactcctg agaaagtcag gaacgaattg aagcaaatgg gctacacatt gagttttgat
     7681 gacagaacaa gtggcccgat aaatgctata tattttgatt ggaaacacgg cagtttctgg
     7741 ggtggcagca gtaaccatgg agaggattat ggaataggct ggtaatacct gcaaaaatta
     7801 aattgtaatg caaatgatct ggtcatattt gctgattggt ggtggaatag tggtgttgct
     7861 gttattgctt gctttgcaaa acaggaaaga caaacggaaa tttattgaca cacttaacca
     7921 ggattacaaa aaacatgaaa aacataagga tgatgaagat ccggatgacc tgaaagccgg
     7981 ctaaacgaac attttttaaa gaagtagcag tatcattcaa taaaggtgac atttcctcat
     8041 actaacaatt gttatctttg catctcaatt ctaaaaatca tatgaaagaa gttgtaatag
     8101 tttctgctgt aagaactccg atgggcagtt ttggaggcag tttaaaaagt ttatcagcca
     8161 cacaattagg tgctaccgct attaaagggg cattacaaaa agccgggatc aaaccagaac
     8221 aagtcaatga tgttttgatg ggttgcgtaa tacaggcgaa tctaggccag gccccggcca
     8281 ggcaggctgc aaaatttgcc ggtttgccca atgaagtgaa ctgcaccaca gttaataaag
     8341 tttgcgccag tggaatgaaa gctattgcac tcgccgcaca aagtattgct ttaggggatg
     8401 cagacattgt tattgccggt ggtatggaaa gtatgagtaa tgtgcctttt tatgctgata
     8461 gtgtaagatg gggtaacaaa tatggtaacg catcattgat agatggtctt gccaaagacg
     8521 gtctgacgga tgtatatgat ggaaaagcga tgggtaatgc agcggaactt tgtgccagta
     8581 catgcggtat cagcagggaa gaacaggatg catttgcgat cgaaagctat aaacggtcac
     8641 agtcagcctg ggaaaaaggc ttatttgcca atgaaattgt acccgtggag atcccgcaac
     8701 gcaaaggaga cccgattgta tttgccaaag acgaagaacc ttttaatgtt aagtttgata
     8761 agataccttc tctcaacccg gcttttataa aagaggggac agttacagct gcaaatgcct
     8821 ccaccatgaa tgatggagct gctgcattgg tattgatgag tgccgacaaa gcaaaagagt
     8881 tgggattgaa accaattgca aagatccgtt cgtttgctga tgcagaacag gcgcccgagt
     8941 ggttcacaac cacaccatca gtagcagtac cgaaagctgt ttccaaagcc ggattgaaaa
     9001 tggaagatat tgcatactgg gaactgaatg aggcatttgc tgtagttggc atagaaaaca
     9061 gtaaacgaat gaaattggat ccattaaaag tgaatgtaca tgggggtgcc gtttcgttag
     9121 gtcatccgct aggctgcagt ggtgcaagaa ttattgtaac actgataaat gtattgaaag
     9181 ccaataatgc gaaatatggt gccgctggaa tttgtaatgg cggaggcggt gcttccgcga
     9241 tggtaattga aaatattgaa taaagggtct cacttttaat taataatctc ttgaagccct
     9301 gtgaaaccag ggctttttcg tacgctgtgt aattagtaaa ttagatatat gattactatt
     9361 cgtatggcca caccagcgga tgcagcactc atcgctaaat tgagccgcca gactttctat
     9421 gagacttttg cagactcaaa cacagcaagc gacatggata aatttatgaa tgaacaattc
     9481 aacaatgagg tgttgatgaa agaagtagaa actcccggcg ccattttttt cctggcctat
     9541 gatgatgaag aacctgttgg ctatgtgcgg ctgagagatg gagaacatca cccggaattt
     9601 aaaggagcat caagcattga aatagccagg atatatgcct taaagaaatc catcggaaaa
     9661 gggattggcc gtgaactgat gcataaatgc atcgatattg ctcgggaaat gaacagggat
     9721 attatctggc tgggagtctg ggaaaaaaat gaaagggcaa ttgattttta tcaaaaatgg
     9781 ggatttgaaa aatttgctga gcatgagttt gtccttggca atgacgttca aacagattgg
     9841 ctgatgtgta aacatctttt caaaaataaa tgacagagag gttttgtatc gtaaactttc
     9901 aactattcaa tagtaaattt tcctgcgcat actagtacta agagcacccc ggaggcgttt
     9961 aaggattgtt taaatgataa aaaaacagca gactgttaaa ccatttgtgt acaacgtccg
    10021 tctgatgtct gtcattttaa taaataataa tctaaacccc aattatgaaa ccaaggatcc
    10081 tttccctcgt aatttcgatt ctttctatat caatactgtt tacagcctgt aagaaagaag
    10141 gctcagtttc acaggattat tctgctgaaa ccgcagcaca ttcagatgat cagtcaagat
    10201 tttctactga agtagatgcc gtggctaatg atgctaatct tgcactggaa gttacaccgg
    10261 ccttttctgg ccggggcgga gaaattcagg cacttatctg cgacgctgat ataacagtcg
    10321 atactttagc taatccaaga actatcacca ttacttacaa cggtaccaat tgtcttggta
    10381 accgtacccg tactggtgtt atcgttttat caatggccca gggtgttcgc tggaagaatg
    10441 ccggagcagc acttagtgta agctaccaga attttaaggt tacccgtgtt agtgataaca
    10501 aaagtatcac cattaacggt tcacaaactt acactaatgt aagtggcggt ttgctgttaa
    10561 atctgccggt tttaggaact attacgcata caatcaccag cagcaatatg tctgtcactt
    10621 ttgataatgg ttcacaacgt acatggcagg tagccaggca aagggtgttt acttataaca
    10681 atggggttgt tatcactgtt accggcactc atactgatgg agccgttaca ggaattgcag
    10741 aatggggtac taatcgtttt ggccgttcat ttataacagc tataacccag ccactggtat
    10801 tgaaacagga ttgtagtttc agggtgggaa gtggtaaagt agtacacaca acacctatgt
    10861 ataatattgc cgctactttt ggactggatg ctaatggtaa cgccacttca tgtcctggta
    10921 ctggttatta ttacatgaaa ctggaatgga caggcccggg gggcaataca caaactgctg
    10981 ttttgccata ttaaaccatt tataacttaa tagaaaccgc cggtatatta ccggcggttt
    11041 ttttatttct ttttatctca cctgaacttt tacaagtaaa ttagcagtta atggcaacga
    11101 ctttaattat caatataaaa ttgctggtaa atacacctgg tgagaacagg ctgttaagag
    11161 ggaaggaatt gtcaaaatta ccttgtattg caaacgctta cctgatcatt gaagatggaa
    11221 taattacagg aattggtgat agcagtgaaa ttcaaaatcc gaaatcaaag ttcaaaaaca
    11281 ttattgatgc tacaggccaa tatgtattac cggcctggtg cgacagccac acccatcttg
    11341 tatttgcagc aagcagggaa gaagaattcg ttgacaaaat aaaaggattg agttatgctg
    11401 atattgcagc caaaggagga ggaatcctaa attctgccaa aaagctgcag actgctactg
    11461 aaactgaatt atttactctt gcatggaaac gactggaaga agttagtaaa cttggtaccg
    11521 gtgctataga aatcaaaagc gggtatggcc tgtcagtaga tgatgaattg aaaatgctca
    11581 gggtaattaa aaaacttaaa gaaaagtctg tgctttccat aaagtctact ttccttggtg
    11641 cccatactta tccgttggag tataaagaca atcaccaggg ttatattgac cttattataa
    11701 atgaaatgtt gccggttatt gcgaaagaaa aactggcaga ctatattgat gtattttgcg
    11761 aaacgggttt cttttctccg gaagaaacag aaataatctg taaagctggt atgcgtagtg
    11821 ggcttaaacc taaaattcat gccaaccaac tgaacttatc cggtggtaca caaacaggag
    11881 taaaattaaa tgccgtaagt gttgatcacc ttgaaacaat ggatgatgag gcaattacta
    11941 ctttggccgg atcaaatact attggaacgt tattgccaac tgctgctttc tttctcagaa
    12001 tgccctttca gcctgcccgg aaactgattg atgccggctg cgccattgct cttgcatcgg
    12061 attataatcc cggttcatct ccatccggca atatgaacct ggtgacagct atgagctgta
    12121 tccagatgaa gatgctgccg gaagaagcta tcaatgcagc tactatcaat ggtgcgtatg
    12181 ccatggaatt gcaggatgaa gtggggagta ttatgattgg caaaaaagca aatctgattt
    12241 ttactaaatc aattccttcg ctggcttacc tcccttacgc atttggttcc aatcttattg
    12301 ataaagtaat gatcagcggt gaattttgtt aaattcaatg tatgcttaaa cttattttat
    12361 gggttttgtc ttctttaatt gcaactgtaa tcttttcaca aaatagcata ccagactgca
    12421 gtttctttaa aaagggagac ttcatctaca aagattcctc cggcgtaaca tgggatataa
    12481 aaagaaccgg caaatatcaa acagaacgga ataagcaaac cggattactt attaagcata
    12541 agatcacatg ggtaagtaat tgtgaatata aactgacaca gatatggtct aataataaag
    12601 acaggagaaa gtacaaccgg tcatggatta tttacaaaat attaagagtt acggataagt
    12661 attattcata ttcctgcact tgtacggacg gcagagagat taccggggtg gtagtgaaat
    12721 taatcgatta atccatttca agaagtgttt agaattggta ccgaatttaa cacatgacta
    12781 tattttatac catacatatt ttgtactttc atatatttta atttgagaaa tttaaaatgt
    12841 atttcgtttt ttaaaaagaa agtaataaaa caattgaata aaagcatctg gatatgtcct
    12901 tacgtcagtt tatttcttca agaaagtttt tacgatcatt tgatattatc aaggaaaacg
    12961 tatcattaaa atggaaagaa gttgccgaat tgttcaacct gcatgaggat gtatttgaaa
    13021 acccccggca tattaggtta aaaaagcctg tttcggttat tcttattggt gctgggcaca
    13081 ggggcggtat ttatgcagat tatgctgttg aaaatcctga tgaaatgaaa attgtttctg
    13141 tatcggatat caacgttaaa cgacgcaata caataaaaaa gctgcacaat attagtgata
    13201 catattgtta cagcaactgg caggatgttt ttaaggtacc gaaatttgca gatgctgtaa
    13261 ttatagccac gcctgatcaa ttacatacac aaccctgtct ggctgcttta gaagcaggat
    13321 atgacgtatt actggaaaaa ccaatagccc caactgaaga agaatgcaga ttgatttata
    13381 ataaagcaca agccagtaat aatattgttg gcgtatgcca tgtacttcgc tattctccct
    13441 atttccgtga attaaaggaa gtattggatg ctgggctgat cggaaatatt atcagtctgc
    13501 agcatatgga gccaattgaa caaattcata tgagtcattc ttatgtcagg ggaaaatgga
    13561 gaaacagtaa gtccgctaca cctattttac tggcaaaatc aagtcatgat actgatatta
    13621 tacgctggct ggtgaatagt cctgctaaag atgttcactg ctttggaaac ctgaaatggt
    13681 ttacgaatat aaatgccccg gaagggagta cggaaagatg tacggatggt tgtaaagtgg
    13741 agtcctcttg tccatactcc gctctgcaga tatatttacg ggataaaaaa agattatatg
    13801 tttttgatat gcccgaaaac aaatcagaat gggatgattt tatactggag aaattaaaaa
    13861 ctactgatta cggaagatgt gtgtaccgta tggacaatga ccagccagac catcttactg
    13921 tgaatatgtt atttgaaaat ggagtaactg ccgccttctc tatggaggca catgtgtcat
    13981 atgagggtag gagaaccagg ataatggggt ctaaaggtga tatagtcgga aatatggagt
    14041 cctttattat gaccgatttt aagaccagga aacaaacttc gtggaaaatg aaaactgatg
    14101 ctcatggtgg tggtgatcac cgattagtaa aggactggat ccaggcagtt gctcaaaatg
    14161 ataaaagcct tctcagttca tcgattgacg tttcgataga gagccacctg atggcttttg
    14221 ccgctgaaaa aagcaggctt aaccggacaa ttgaagaagt taaattaatc agtaatgaat
    14281 atcttataga aaacggataa tcaccggttc tgcctggtaa ttaattacgg ttcatccgat
    14341 aaaggaatct catcattgat ggcttgcagc ttaggtttcc catatgcaat ggacccaatc
    14401 atcataatga tacttgtaat taagatcaca aagaataata ttccctcatt aaactttgat
    14461 aatttataaa gttgggggat gctatagaat aaaaggatag tgatcagacc tctcggcatg
    14521 aaaaaaactt caggaacaat atgtgaccgg agaaaaaacc gcaggtaaat aaaccggata
    14581 aacaacagta aaataacaat ggcactgcca gccagtataa catccttatc catgattaac
    14641 ctgatatcaa tagttaatcc aaaaatgaag aagaaaaaag tcctgatcag gaagctgctt
    14701 tccgctgtta atgaatggag taaatgactt acatcatcta cctgttgaat ggatacccat
    14761 ttgtagagtc ggttaaaaat tgttagttgc cagttactta caattaatcc aaataacagg
    14821 ataatgaata aagatggcag atgcagcatt ttgccgccag catataaaaa tattaaaaca
    14881 gcaaatacca ggaagaacct tactttggtt gtaatccggg taagcataaa tagcataaga
    14941 aatgatagta caatacttaa aacaattgaa ataatcaatg atccgaaaaa ccaggtaata
    15001 tttgaacctt ttaataagtt gcccgctata agataattaa agaaaatgat accaaggata
    15061 tctgaaaatg aagtttcata aacaagaaat tcctttttat gctcatccag gtgagaaata
    15121 ctgggtataa caatagcact gctcacaaca gaaagaggta atgcataaat caggcaaaga
    15181 ataaaatctt cttttagata gaaatacagg aacgaagaaa tagcaattgc tgaaagtact
    15241 aatatgatca aacctgaaaa aaatgcatta cggatcaatt ttattttctt tttccccaac
    15301 tgtaaatcca atccagcctc aaggatgatc ataattaaac ccagaatacc aaatatctct
    15361 accatttgtg gtgaaacttc gactgaaata ttattaaata caagggtttc ccgtacccca
    15421 ataccagtca gcaataacag taaaactgaa ggtattccag tccgccggga aatcaggttg
    15481 aataaatacg acaggatcac taaaaggcaa atggctatta tatatccgta tgcaccaatt
    15541 tctttcataa ttcaaaaata tcagaaataa tcaatagaaa aatactttta tcatttctta
    15601 acttaagcaa aaaattcatt tgtcgtattt ttacagagcc cgcatagtat ttttaatttg
    15661 aatttatatg tatagtaagc aggcatacga gatatttctt tcagccacga tggcagttca
    15721 gccgcagttg ttattacctg aatacattta ttcaaaaaac ggatttctcc atttaacgga
    15781 tcaagagttc aatatccggg atatcaatag atttatagta attgctgctg gaaaagctgc
    15841 accgggaatg gccaatgtta cagaaaaaca agttggtatt ttagtttcag aaggatattg
    15901 cattactaaa tacggacatt cccttcccct ggagtatttt catactattg aagctgctca
    15961 tccggtgcca gaccaaaata gtattaaagc agttgagata gtattacaag ctgtaaatgg
    16021 tttaaatgtt gatgatattg tgctggtatt acttagcgga ggcgcttcct cattgctggc
    16081 agatattcct gaagggtgta cactggaaga aacaaagaat atatttaaaa tgctgatcaa
    16141 tagcggggca tccatccatg aaattaatat tgtaagaaag catttaagta agattaaagg
    16201 tggacaactt gcaaaagcgg tttatccggc aaagcttttt acactgatta taagtgatgt
    16261 ggttggggat cacctggaaa gtattgccag cgggcctacg gtctccgaca gctctacatt
    16321 tgaagacgct tatcggattt tacaaaaata taatatctgg acaagcacag cagaaagtgt
    16381 cagaaaatat attctaaagg gagtaaatca aactattgaa gacaccccca aacccggtgc
    16441 tcactttttt tacaatacga ctacaaaaat tatcggtagt aataatcttg cgttgaatat
    16501 ggccaaacaa aaagcagaac aatttggcta tcactccttt attttgacag acaatctaac
    16561 cggaaatgct gatacggaag cccgcaaatt tgtccactac ctgcaacatt attctggcga
    16621 aaaacctgta tgtattctga tgggaggaga aacaacctta aaagtgacaa gccccggcaa
    16681 gggtggccgt aatcaacatt ttgttttaag tgcaataaat gaattgctaa aaaatagtat
    16741 atctggtacc gatcatgata taacaattct aagtgccgga acagatggta cagatggacc
    16801 tactgatgct gcaggggctt tatttaacaa taaatccctg cccgctttca tgggaatgaa
    16861 tgaaatgata caggagcacc ttgatcgatt cgattcctat agttatttct ataaaatggg
    16921 tggtctggtt ataacaggac ccacacaaac aaatgtcatg gatattgtag tggcattgat
    16981 acattaattc aatataattt tatagtaata tcggattaat aattgttaat tgatagcaaa
    17041 gagtgtctgt gccctgcctt aac
//
LOCUS       DAORTI010000072        16660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3177_length_16660_cov_7.193737, whole genome shotgun sequence.
ACCESSION   DAORTI010000072 DAORTI010000000
VERSION     DAORTI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_16660_cov_7.193737"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLO70_10080"
     CDS             <1..353
                     /locus_tag="PLO70_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74858.1"
                     /translation="NYELKNIADFKAGKQYDAVGLIFIHLPEVLRKKFHQEVYNSIKP
                     GGFMVLEAFAKEQAQLESGGPRDATLLYDVPSLCNDFPFLHMLSCEQKEIFLDEGDYH
                     KGKASVLRMIGQRL"
     gene            complement(356..673)
                     /locus_tag="PLO70_10085"
     CDS             complement(356..673)
                     /locus_tag="PLO70_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HQZ74859.1"
                     /translation="MDQTEKRINKDLILREKLAIERTEMAIDRTLLSFIRTSLYFVIA
                     GMTVNSLLKVSYGWWVEILFWFLGIVVLITGIIKYRQQKKKLKNNEKHIGDYKLEWED
                     DIE"
     gene            complement(660..1247)
                     /locus_tag="PLO70_10090"
     CDS             complement(660..1247)
                     /locus_tag="PLO70_10090"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HQZ74860.1"
                     /translation="MHKRYLIISLLLVSIISCTEKKETTDLSALKSKGDSLIAQTFDT
                     LRNTLLSTIGEKGFPGAVSFCNTKALSLTNTYASEGIIIKRTSDKLRNPANRPDNMEQ
                     EILSAYLQMKEDKQELKPVLKKDTEGNHHYFKPILLQAMCSNCHGDKNTQIKPDTWEA
                     IRLQYPADAAYNYKEGDLRGIWHLTFLKNKINGPN"
     gene            complement(1264..>1560)
                     /locus_tag="PLO70_10095"
     CDS             complement(1264..>1560)
                     /locus_tag="PLO70_10095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ74861.1"
                     /translation="ILGLGTALVYPNFLTVVAESTHPSQRAETLSIFRFWRDSGYVIG
                     ALLAGFLADMIGMSIAIIIIAGITATTGVVAHFRMCCTLKKLYSSEDCIRPSLY"
     assembly_gap    1561..1660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1661..2376)
                     /locus_tag="PLO70_10100"
     CDS             complement(<1661..2376)
                     /locus_tag="PLO70_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ74862.1"
                     /translation="MGNLKAGLKENWQQFSLLVLVNAFVGAMIGLERAVLPGLGKSVF
                     GLDANSAILSFIMAFGITKAVSNYSVARLSKRFNRKQILMAGWFAALPVPFLLMYADS
                     WSWVIIANILLGINQGLAWSSTVIMKVDIVGQKNRGLAMGINEFAGYLSVGLASYLAS
                     SLASTYGYAYYPFIPGLFFASAGLLVSAFLVKDTTHFVHAESVTSKIALLKNVWKETT
                     WKHKNIGSVTLNGLVNNMNDA"
     gene            complement(2443..3021)
                     /locus_tag="PLO70_10105"
     CDS             complement(2443..3021)
                     /locus_tag="PLO70_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ74863.1"
                     /translation="MDKTELAQLFQLFKKYNALPVKAEEAIAAISSIITVKKNKDLQP
                     IGHTCKTIYFINKGIARIYYFKDGIDITENFFFENNIIARVESLFTGKPSRKAIQILE
                     DAEIVGINANQLFKLYDSFPEIERLFRKIFEAAYVEKVHRIEGIQFHTAEERYKALLQ
                     EAPDILKRVPLKYVASYLGITQVSLSRIRGLK"
     gene            complement(3060..>3545)
                     /locus_tag="PLO70_10110"
     CDS             complement(3060..>3545)
                     /locus_tag="PLO70_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74864.1"
                     /translation="LIDITENGYNQVTEFNQQFSHYHTATLPVYSKTNKILYSIFLGG
                     IAQRYTDSAGKVITDNDIPFVKSISVVERKGNSIEEYILPDPMPGYFGAAAEFIPADE
                     NLFTPNGILDLDKAGKKPLMAGYIIGGIDSRAPNVFWSNEADPSRASPVIWKVYIRKN
                     H"
     assembly_gap    3546..3645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3646..4534)
                     /locus_tag="PLO70_10115"
     CDS             complement(<3646..4534)
                     /locus_tag="PLO70_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74865.1"
                     /translation="MRIFFWLITSLVITVTINAQSSYPFQITLEPVIPEGFAGLQSYA
                     WAKDGDKVLLAGGRTDGLHRRQPFASFNKKYNNTELIVLDLKQEKVWKKNIFSLPVSF
                     AEQLQSSNMEFFQQGNQLLIIGGYGYSETKRDHITHPAVISIKVKELINAIITSQDIS
                     PFIVQLIDERMAVTGGRLNKLGNLLYLVGGQRFDGRYNPHGPDHGPGFSQQYTNQIRK
                     FTLTEKKGSLSIDNYTAISDSQLLHRRDYNLLTQFDEKGKEMLTIYSGVFQYDKNVPF
                     TTLIDITENGYNQVTEFNQQ"
     gene            complement(4538..5236)
                     /locus_tag="PLO70_10120"
     CDS             complement(4538..5236)
                     /locus_tag="PLO70_10120"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HQZ74866.1"
                     /translation="MKVFLSIVTVIVLFFLPLPVEVKTAGELKKIMREGNLSAHISLD
                     TLLTNPHMYGLGPADGIKGELLIIDSKAFWCKVENEKINTSMNNNAKAAMLVYAQVLA
                     WKSVTITDEVKDYAALEKLVEKFSLQNGQSLDIPFPFMIKGIANSASWHVINWKEGVT
                     HTFDNHKQFAKYGSFNNEEVTILGFYSNKHHSIFTHHTTNMHIHVTDKKNTTVGHLEE
                     ILAAANTLTLLIPQ"
     gene            complement(5251..5724)
                     /locus_tag="PLO70_10125"
     CDS             complement(5251..5724)
                     /locus_tag="PLO70_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HQZ74867.1"
                     /translation="MKTNRIGLDATKARELAEKLNLLLANFQLFYMNSRGFHWNIKGE
                     KFFELHLKFEELYTDSQLKIDEIAERILTLGHTPSHSYTDYLKLSKIKEKRNVTDGKE
                     AVKSVVEAFGALLDIERELLSIAADAEDEGTNALMSDYIRQQEKQVWMYSAYLDK"
     gene            complement(5892..6341)
                     /locus_tag="PLO70_10130"
     CDS             complement(5892..6341)
                     /locus_tag="PLO70_10130"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQZ74868.1"
                     /translation="MYNYEVDLAWKESRKGVISSPVLTQSIEVATPPEFQKGIENIWS
                     PEHLFVAAINSCLMTTFLAVAENSKLEFESFNSQANGKLDKVDGKFMMTEVLLRPTLV
                     INNPEMQDKAKRVLEMSEKACLISNSVKSTIIFDPTILIQEKEVVII"
     gene            6699..7934
                     /locus_tag="PLO70_10135"
     CDS             6699..7934
                     /locus_tag="PLO70_10135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ74869.1"
                     /translation="MKHLVILGAGTAGTMMANHLNHHLKKPDWQISIIDERKEHHYQP
                     GYLFLPFDIYSPEDIIKNIEDFIPKQVELIKATIDKVLPKENKVKMADGRELNYDVLI
                     VATGAKIAPEETEGMKGSEWQKSVFDFYTFEGSLALRNKLRDWQGGKLVVHITEMPIK
                     CPVAPLEFAFLADSFFKHKHMRDKVEITYVTPLSGAFTKPKATEALEHLLHDKNINIE
                     SDFAIEHVDNEKKQIVDYGGREIPFDILVTVPTNKGDAVIERSGMGDDLNYVPTHKAT
                     LQSKEYSNVFVLGDASNIPASKAGSVAHFEAEILTDNILHFIKGEPLKEEFDGHANCF
                     IETGNGKALLIDFNYTHEPVEGSFPFPGVGPLRLLKESRMNHMGKLAFRWIYWNVLLK
                     GTHIPFVSATMSESGKHYA"
     gene            7962..8270
                     /locus_tag="PLO70_10140"
     CDS             7962..8270
                     /locus_tag="PLO70_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HQZ74870.1"
                     /translation="MEKIIAGKSIAINEEGYLTDFKQWDKSIGEELAAEAEISLTPRH
                     WEVLTYLQTEYKNEVPLSIRKIGKSGVVDIKEFYQLFPKAPLKTATKIAGIPKPASCI
                     "
     gene            8294..8776
                     /locus_tag="PLO70_10145"
     CDS             8294..8776
                     /locus_tag="PLO70_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQZ74871.1"
                     /translation="MSENKGEIKKMMIILSKGTLENVYAAFVLANGARMEGIEAEMFF
                     TFFGLEAVHKKKLTHLHVATVGNPAMHIPTMLGGLPGVEALATTMMKKEMDKVDMPDV
                     AEFLDILSASGVRMWACKLAMDMFHYKKEDLFEGVEDVITVGDFYKQSAGEGTHMLFI
                     "
     gene            complement(8847..9386)
                     /locus_tag="PLO70_10150"
     CDS             complement(8847..9386)
                     /locus_tag="PLO70_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQZ74872.1"
                     /translation="MNLNTQEPISEKKHHEFLTENTDFEVRSLDAVCFNESELNHPWW
                     YNFKYAIAGLLFGVILVKAEVISWFRIQEMFRLQSFHMYGVIGSAVAVGMLSVFIIKK
                     FKIKTIYNEPIEFVDKKLNKGNIYGGLLFGFGWALTGACPGPLFAQIGSGATVIIVTL
                     FSALVGTWIYGYFREKLPH"
     gene            complement(9396..9959)
                     /locus_tag="PLO70_10155"
     CDS             complement(9396..9959)
                     /locus_tag="PLO70_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQZ74873.1"
                     /translation="MLEWIKQPWPWYVAGPLIGLTVPALLLVGNKSFGISSSLRHACA
                     ACLPANIPFFKYDWKKEIWNLFFVAGILAGGLIAAQLLSNPGPVQVNAKLAEEMVTYG
                     VTNYEGLVPKDLFAWPELLTARGIIMMVGGGLLVGFGTRYAGGCTSGHAIMGLSNLQF
                     PSLITTISFMAGGFIVANLVLPFSLKL"
     gene            complement(10039..11040)
                     /locus_tag="PLO70_10160"
     CDS             complement(10039..11040)
                     /locus_tag="PLO70_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ74874.1"
                     /translation="MWKHIKSNWQLIIIIIVIVIVALTEIMKAIKPSGKQAFTAMPED
                     STWIPPSLFTDNLLEGEEREMVIYGEDIIANTSRYFGPHGSVAPITNGMNCQNCHLIA
                     GKKSWGNNYSVVFSSYPKFRDRSGATETVYKRVSDCFERSLNGTAPDSTSREYKAIYS
                     YIKWLGKDVKKGEKPVGSGIEKILYMDRAADPIKGKGIYTAQCQSCHGANGEGQLSLN
                     GISYEYPPLWGPHSYNDGAGLYRLSNFAGYVKSNMPFNQTTHSNPKLSNEEAWDVAAY
                     VNSQPRPKKDLSKDWPDISKKPFDHPFGPYTDGFNETQHKYGPYKPIIAKKEELTKK"
     gene            complement(11059..12762)
                     /locus_tag="PLO70_10165"
     CDS             complement(11059..12762)
                     /locus_tag="PLO70_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HQZ74875.1"
                     /translation="MSSLLKNDSPNSRRDFLIKTTLAGLGTSLAGVAGAIPLTNSETK
                     QLTPETSTRFTILYTSDIHAQLHTHDEFFWENGKAVYKKRGGLAVLKTMINHFRKQNP
                     ANTLLIDGGDYFHGSAVASLTEGEALIPLLNDFNYDLILPGNWEVVYKKKKMLYDMGH
                     ANAAKICANMWHKTTDADNGELIYPPYWVKYIAGTKVGFIGYTDHLIPKRQSPAYSEG
                     IRFEHADFNVAKYIKLLKEVEGCGIIFIVTHMGLAQQVGLANNPAVAGADFILGADTH
                     ERVRVPIEGKYSKVVECGAFGSFLGKLDIEVENGRIKNYKYQLLDVDPAKYPADKKLQ
                     SKVDKASAPFHQDLSKVIGTTTTPLVRYFVMETPMDNLITDAIHWKFKTDIALSNGFR
                     FCQPLAVDPKKGKADITREFLWNMLPVDSEAKLGIITGKQLWDWQEKELQNAFAKDPS
                     KRFGGWFVRFAGMEVNFTIGNELGKRVNWIKVKGEPLDINKEYSIVACEREGDPDDTL
                     CRIEKVKNPKRSGVLMHDVIEEYLAAHSPVSPKLERRATATDQPHTLLTQLQGTTYEF
                     R"
     gene            complement(12864..14027)
                     /locus_tag="PLO70_10170"
     CDS             complement(12864..14027)
                     /locus_tag="PLO70_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQZ74876.1"
                     /translation="MFRKFLKYFAILLIITTIAFFAFAPSYIDKSKNKVTLKVVNVQK
                     VNWYDSIPFIADLHCDALLWDRNNLKHHSYGHVDIPRMQEANMAFQVFTIVSKTPKGL
                     NIEKNDDKTDQIALLSFAQLRNPKDWFSVKSRALNQCRELYKSAKSSNGEFRVITNQE
                     ELQQYITDRRINKKLTAGMLGIEGAHCLGDDINNLQLFYDAGVRYIGPTHFFDNSWAG
                     SAHGINKGGLTEKGKQLIRKMDSLHMVIDLAHASPKTIDDIFVLTQSPMLISHTGVKG
                     VCDNNRNLSDAHLMEIGRRKGLVGIGLWETAVCGTDADATAKSIRYVADKIGVDKVAL
                     GSDFDGAITTHFDVTGLPLIVNALLKEGFNKEEIYQIMGGNIRDFMLKNLPGR"
     gene            complement(14081..15499)
                     /locus_tag="PLO70_10175"
     CDS             complement(14081..15499)
                     /locus_tag="PLO70_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HQZ74877.1"
                     /translation="MKKIFIFLGFTITMSAAVAQHQEVNEKPGLWKEKESETDSTSIL
                     SAFRRGTVSGHLRYYFMSTNNKADLTDYYAHAVGGGIKFETGKFKNFQLGVSGFFVYN
                     IGSADLTIPDPKTNQMNRYETGLFDIEDPANKKDIDRLEELYIKYHFKKSALTLGKQL
                     INTPFINLQDGRMRPTEVEGLWAEINGIKKIKLQLGYLYGISPRSTVKWFKVNESIGV
                     YPVGVNENGIKSGYQGNLESKGIILTGITYQHNKNLKLQLWNQFTENIFNSTFLQADY
                     EYPLDGKSQLLVAAQVIRQDAINDGGNEDPAKAYYKKGAKAFTFGTKLGWKNVNWETT
                     INYNRITAHGQYLMPREWGRDPFFTFLPRERNEGLGDVHAIVGKVNYKISKARITTSV
                     AFGNYNLPEVTEFSLNKYGIPSYNQLNIDIRYKFAGLLKGLETQLLYVFKEKTGNSYG
                     NDKYVINKVDMSLWNLVFNYQF"
     gene            complement(15560..15991)
                     /locus_tag="PLO70_10180"
     CDS             complement(15560..15991)
                     /locus_tag="PLO70_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQZ74878.1"
                     /translation="MYRKFFIAVVITLFVTQLQAQNISTTTSQHRIIMQLSSNDTLVW
                     KGLMNNLKHLKAGWGDSLIIEVVAHGPGIDLLTKGKTTQQERITHFKKMGIDFIACEN
                     TMMERKVPKESIIAEATFVKMGIGEIVRKQEQGWSYIKIGF"
     gene            complement(16014..16436)
                     /locus_tag="PLO70_10185"
     CDS             complement(16014..16436)
                     /locus_tag="PLO70_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ74879.1"
                     /translation="MALLLPNIKKKSLLIIAGGLLLAGLCSTVCVQAQDNTAGKTVSQ
                     NKFQRLINKKNTILLDVRTEVEYKAGHIPGSLQIDFLKTEDFKKQVAVLDKSKPYLLY
                     CRSGKRSKDAMTVMKEMGFTKLFDLQGGFNSWEGVKEQ"
     gene            complement(16447..>16660)
                     /locus_tag="PLO70_10190"
     CDS             complement(16447..>16660)
                     /locus_tag="PLO70_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74880.1"
                     /translation="KSIKDALKKMAGIQEVEVYKEEEKICIMGVGINRGLLIEKLAAM
                     GYPEKGNNTLMSKAKSFISCAAGKLN"
BASE COUNT         4704 a         3282 c         3094 g         5380 t
ORIGIN      
        1 ttaattatga acttaaaaat attgctgatt ttaaagcagg taagcagtac gatgctgtgg
       61 gattgatttt tattcatttg cctgaggtat tgcggaaaaa atttcaccag gaggtatata
      121 attctattaa acctggtggt tttatggtac tggaggcatt tgcaaaagaa caggcacaat
      181 tggaaagtgg aggcccaaga gatgcaacgc ttttatatga tgtgccatca ctatgtaatg
      241 attttccttt cctgcatatg ttgagttgtg aacaaaaaga gatttttctg gacgaaggag
      301 attatcacaa agggaaagca tcagttctgc ggatgatcgg tcagcggttg tgatattatt
      361 caatatcatc ttcccactca agtttgtaat cgcctatatg cttttcatta tttttcaatt
      421 ttttcttttg ttgcctgtac tttattattc cggtaataag tacaacgatg cccaaaaacc
      481 agaaaaggat ttctacccac cagccataac tcactttcag caggctattc actgtcattc
      541 ctgcaataac aaaatataat gaggtcctga taaaagataa taaggttctg tcaatagcca
      601 tttcagttcg ttctattgcc agtttttccc tgagtataag gtctttgttt attctctttt
      661 cagtttggtc cattgatttt attttttaga aaagtcagat gccagatccc tctaaggtca
      721 ccttctttat aattgtacgc tgcatcggcg ggatattgta gccggatagc ttcccaggta
      781 tccggtttta tctgggtgtt tttatcgccg tgacagttac tgcacattgc ttgcaaaaga
      841 atgggtttga aataatggtg atttccttcg gtatcttttt tcaacactgg ttttaactcc
      901 tgtttatctt ctttcatttg taagtaagct gataatattt cctgctccat attgtcgggt
      961 ctgttagccg gattacggag tttatcagaa gttcgtttta ttataatccc ctctgaagca
     1021 taagtgttgg ttaatgacag tgcttttgta ttacaaaagc tgacagcacc cggaaatccc
     1081 ttttcaccaa tagtacttaa tagtgtattt ctgagtgtat cgaatgtttg agcaattaat
     1141 gaatctcctt ttgatttcag cgcagacaga tcggtagttt cctttttttc agtacaggag
     1201 atgatagata ctaacagcag gctaataatg agatatcttt tgtgcatgtt ataaaattac
     1261 tgattaatac aatgatggcc tgatacagtc ttcagaagaa tacagctttt ttaatgtaca
     1321 gcacattctg aaatgggcaa cgacacctgt tgtggcggta ataccggcaa tgataattat
     1381 ggcaatgctc atccctatca tatctgccag gaagcctgct agaagggcgc cgatcacata
     1441 tccactatcc ctccagaacc ggaaaatact cagggtctct gccctttgtg agggatgagt
     1501 actttcagcc actaccgtaa gaaagtttgg atataccaat gcagtaccaa gtcccagtat
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcatcgttca tattattcac
     1681 caggccattt aatgtaacac taccgatatt cttatgcttc caggtcgttt ctttccatac
     1741 attttttaat aatgctattt tactggttac actttcagca tgaacaaaat gagtagtatc
     1801 ctttaccagg aaagctgaaa ccaacagccc ggcagatgca aaaaataacc ccggaatgaa
     1861 agggtaataa gcatagccat aagtggaagc gagggagctg gcaagatatg aagccaaacc
     1921 tactgataga tatcctgcaa actcattaat gcccattgcc aatcctctgt tcttttgtcc
     1981 cacaatatcc actttcatta taactgttga ggaccacgcc aatccctggt tgatacccaa
     2041 taaaatattt gcaattatca cccatgacca cgaatccgca tacattagta gaaatggaac
     2101 tggtaaggct gcaaaccagc ctgccattaa tatttgtttc ctgttgaatc gtttgctgag
     2161 tctggctaca ctataattac taacggcctt tgtaatacca aatgccatga taaaacttaa
     2221 gatcgctgaa ttggcatcca gcccaaatac acttttccct aatccgggca acacggctct
     2281 ttccaatccg atcatggctc cgacaaaagc attgacgagc accaaaagtg agaattgttg
     2341 ccagttttct ttcaagcctg cttttaaatt tcccatttgt gagattttgc ttcacaaagg
     2401 tcatcacctt aaacagctat tcaattatca tatgataaat aatcacttca gtccccggat
     2461 tcggctcagg ctaacttgtg tgatcccgag gtaagaagca acgtatttca atggcaccct
     2521 tttgagtata tccggtgctt cctgcagcaa ggctttatat ctttcttcag cagtatgaaa
     2581 ttgtatcccc tcaatgcgat gaactttttc aacataagcc gcctcaaata tttttctgaa
     2641 aagcctttct atttccggga aggagtcata taatttaaaa agctgattgg cattaatacc
     2701 tactatctct gcatcttcga gtatctggat ggctttacgg ctgggttttc ctgtaaataa
     2761 actttctacc ctggcaatga tattgttctc gaaaaaaaaa ttctctgtaa tatctattcc
     2821 atccttgaaa tagtaaatgc gggcaatgcc tttattgatg aagtatattg ttttgcaggt
     2881 atgaccgata ggctgcaggt ctttattttt ctttactgta ataatactgc tgatagcggc
     2941 aatggcttct tctgccttta caggaagtgc attatacttt ttaaaaagtt ggaataactg
     3001 cgccagctcc gttttatcca tacctgtaaa ggtaggaaca gaaagttgat agcttcttcc
     3061 taatgatttt ttctgatata gactttccag ataacaggtg aagctctgct gggatccgcc
     3121 tcattactcc aaaatacatt gggggcacgg ctgtcaatac caccaatgat atagccagcc
     3181 atcaatggct ttttaccggc tttatctaaa tcgaggatac catttggtgt gaagagattt
     3241 tcatcagcag gaataaactc tgcagcagct ccaaaataac ctggcatcgg gtcaggaagt
     3301 atatattcct caatagaatt tcctttacgt tcaaccacac ttatactttt tacaaaaggg
     3361 atatcgttat cagtaataac tttcccggca ctatcagtat atctttgtgc gataccaccc
     3421 aaaaagatgg agtataaaat tttattagtc ttactgtaaa caggcaatgt agctgtgtgg
     3481 taatgactga attgctgatt aaattctgtc acttgattat atccgttctc agtaatgtca
     3541 atcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattgc tgattaaatt
     3661 ctgtcacttg attatatccg ttctcagtaa tgtcaatcag tgtggtaaag ggcacatttt
     3721 tatcatattg aaacaccccg gaataaatgg tcagcatttc cttgcctttt tcatcaaact
     3781 gtgttaacag gttataatct ctccgatgca acaactgaga atcggagatg gcagtataat
     3841 tatcaatact gaggctccct tttttttcag taagagtgaa ttttctgatc tggttcgtgt
     3901 attgttggga aaatcctggg ccatgatcag gcccgtgagg gttataacgg ccatcaaatc
     3961 gttgacctcc tacgagataa agcaggttgc caagcttgtt taaccggcct cccgttacgg
     4021 ccattctttc atcaatcagt tgaactatga atggactaat atcctgacta gttataatag
     4081 cattgatcag ttctttgact ttgatactga ttaccgccgg gtgagtgata tgatctcttt
     4141 ttgtttcact atagccatag ccaccaatga ttaaaagctg attaccctgc tggaaaaatt
     4201 ccatattaga gctttgcagt tgttctgcaa aagatactgg aagtgagaag atattctttt
     4261 tccatacttt ttcctgtttc aggtccagca caatcagttc tgtgttatta tatttcttat
     4321 tgaaggacgc aaaaggttgt cgccggtgca gaccatcggt cctgccacct gcgagtaata
     4381 ctttatcacc atcttttgcc catgcataag actgaagacc tgcaaaaccc tctggaatta
     4441 caggttcaag tgtaatttga aacggataag acgattgtgc gttaatagta acagtaatta
     4501 ccaggcttgt aatcagccaa aaaaagattc tcatgtttta ttgagggatt aatagggtta
     4561 aagtatttgc agcagccaat atttcttcca ggtggcctac agttgtattt tttttatcag
     4621 ttacatgaat atgcatattg gtagtatgat gggtgaatat actatgatgt ttgttggagt
     4681 aaaaccccag aatagttacc tcctcattat taaagctgcc gtatttagca aactgcttgt
     4741 gattatcaaa tgtatgtgtg actccttctt tccagtttat aacatgccag gatgctgaat
     4801 tagcaattcc ttttatcatg aatggaaaag gtatatcaag actttggcca ttttgcaatg
     4861 aaaatttttc taccagtttt tcgagtgctg cataatcttt tacttcatct gtaatagtaa
     4921 cagatttcca ggcgagaacc tgtgcataca ccagcattgc tgcttttgca ttgttattca
     4981 tggatgtatt tatcttttca ttttctacct tgcaccagaa ggctttacta tctattatca
     5041 gcagctctcc ctttattcca tctgcaggtc caagaccata catatgcggg tttgtaagaa
     5101 gtgtatccag tgatatatgg gcagaaagat ttccttcccg catgattttt ttcaattctc
     5161 ctgcagtctt tacttctact ggcaatggca gaaaaaaaag aacaataact gttactatag
     5221 ataagaatac tttcattgac tgcagattaa ctatttatcg aggtaggcag aatacatcca
     5281 gacctgcttt tcctgctgac ggatataatc actcataagt gcatttgtac cttcgtcttc
     5341 tgcatcagca gcgatactta gcagttcccg ttcaatgtcc agtaaagcac caaatgcttc
     5401 aaccactgat tttaccgctt ccttaccgtc cgtcacattt cttttttctt ttatttttga
     5461 taatttaaga taatcggtat acgaatggct gggagtgtgt ccaagggtta aaatcctttc
     5521 agcaatttca tcaattttaa gttgtgaatc agtatacaat tcttcaaatt tcagatgaag
     5581 ttcaaaaaat ttttctcctt tgatattcca gtgaaagccg cgggagttca tataaaaaag
     5641 ctgaaaattt gcaagtagta aattaagttt ttcagctagt tctcttgctt tagtggcatc
     5701 cagcccgatt ctgtttgttt tcatgtttca acaatttatg gagtgtctaa attaactttt
     5761 ttgtacaggc tggctggtga tttttatcac gattgtacag aatatgttat gaataaggcc
     5821 ggagaattgt ttctccggcc aggattaaga tattcgttta tgaggtacta tgagaaattt
     5881 aaacatgctg attatatgat tactacttct ttctcctgta ttaaaatggt agggtcgaaa
     5941 ataatggttg atttaacaga gttggatatt agacaggctt tttcactcat ttccagtact
     6001 cttttagctt tatcctgcat ttcgggattg ttaattacta atgttgggcg aagcagcacc
     6061 tctgtcatca taaatttccc atccacttta tcaagtttgc catttgcctg gctgttaaat
     6121 gattcaaatt ctaacttaga attttcagca acagcgagga atgttgtcat aaggcagctg
     6181 tttatagcag ccacaaaaag atgttcgggt gaccagatat tttctattcc tttttgaaat
     6241 tccgggggtg ttgccacttc gatggattgt gttaaaacag gagagcttat tactcctttt
     6301 ctgctttctt tccaggcaag atcaacttca taattgtaca tgattctatt atttaaggtt
     6361 tgcaataatt attaatattt cagactattt ttaagataat cttgaatttt acttctataa
     6421 aatcagaaca aaactatact aatacacttc agatgaaggt gattaatatc accccaggag
     6481 ataatccaaa accggtaaaa ttcagtttta tagtaagcgt ttatttagtt agtggtgact
     6541 tatatcacat agcaaataaa ataaactgta aatggaggaa aatcatttcg ttagttgata
     6601 tgagtcattc agttttgtga ctcaactcgc agtacaaggc acccgggaaa tcgaatttta
     6661 caccgtaaat atttcttagc tcaatcaaac aaaaaaatat gaaacatctc gtgatattag
     6721 gtgcaggaac tgccggaacc atgatggcaa atcatttgaa ccatcattta aaaaaaccag
     6781 attggcagat aagtattatt gatgaaagga aagagcatca ttatcagcct ggttaccttt
     6841 ttctcccttt cgatatctat tctcctgaag atattattaa aaatattgag gattttattc
     6901 ccaagcaggt cgaattgata aaggctacta ttgataaggt tttgcctaaa gaaaataaag
     6961 taaaaatggc agatggccgg gaactgaatt atgatgtcct gattgtggct accggggcaa
     7021 aaatcgcacc ggaagaaaca gagggaatga aagggtctga gtggcaaaaa tcggtttttg
     7081 atttttatac ttttgaaggt tctcttgcat taaggaataa attaagggat tggcagggtg
     7141 gaaaattggt agtccatata acagaaatgc caataaaatg tcctgttgct ccattggagt
     7201 ttgctttttt agctgattct ttctttaaac ataagcatat gcgggataaa gtagaaataa
     7261 cctatgttac cccgctcagc ggcgctttta ccaaaccaaa agctacagag gctcttgaac
     7321 acctgctgca tgataagaac attaatattg aaagtgattt tgctattgag catgttgata
     7381 atgagaaaaa gcagattgta gattacggcg gacgggaaat cccttttgat atcctggtta
     7441 ccgttccaac taataaaggt gatgccgtta tagaaagatc cgggatgggt gatgacctga
     7501 actatgtacc cacccataaa gcaacattac aatcaaaaga gtattctaat gtatttgtat
     7561 tgggtgatgc cagtaatata cccgcatcta aagccggttc tgttgcgcac tttgaagcgg
     7621 aaattttaac tgataatatc ctgcatttta tcaaaggaga accattgaaa gaagaatttg
     7681 acgggcatgc caattgcttt attgaaaccg gaaacggcaa agcgctgctt atcgacttta
     7741 attatactca tgaaccggta gaaggaagtt ttccatttcc cggcgttggt ccgctccggt
     7801 tattaaaaga gagccggatg aatcacatgg gtaaactggc cttccgctgg atttactgga
     7861 atgtattgtt gaaaggaacg catatacctt ttgtttctgc aacaatgtct gaatccggga
     7921 aacattatgc ttaattattt aatcattata aaaaaacaat catggaaaaa ataattgctg
     7981 gcaaatcaat tgctataaat gaggaaggat accttaccga ttttaaacaa tgggacaaat
     8041 ctattggtga agaattagct gcggaagctg agataagcct gacccccagg cattgggaag
     8101 tattgaccta tttgcaaact gaatacaaaa atgaagtacc cctgagtatc cggaagatcg
     8161 ggaaaagcgg ggtagtggac attaaggagt tttaccagtt gttccccaaa gcaccactga
     8221 aaacggctac taagatcgca ggaattccaa agccagccag ttgtatttaa taatttatta
     8281 aaacttatca atcatgagtg agaataaagg tgaaattaaa aagatgatga tcatcttgtc
     8341 taaggggaca ttagaaaatg tttatgcagc ttttgtattg gcgaatggag ccaggatgga
     8401 agggattgaa gctgaaatgt tttttacttt ttttggactg gaagccgttc ataaaaagaa
     8461 attaacgcac ctgcatgtgg caacggtggg aaatcctgct atgcatatac caactatgct
     8521 cggtggtctg cctggagtag aagcattagc tacaacaatg atgaaaaagg aaatggataa
     8581 agtagatatg ccggatgtag ctgagttttt ggatattctt tcagcatcgg gtgtacggat
     8641 gtgggcttgt aaactggcga tggatatgtt ccattataag aaagaagatt tatttgaagg
     8701 agtggaagat gtaattacag ttggtgattt ctataagcag agtgccggag agggtacaca
     8761 catgctattc atctgagaat acaattgcta tataaaataa tagcgaccaa tactggtcgc
     8821 tattatttta tcaaaagtat ccctttttaa tgcggtaatt tctctctgaa gtatccataa
     8881 atccaggtac ccaccaatgc actgaacaag gttacaataa taacagtagc accggagcca
     8941 atctgtgcaa ataatggtcc ggggcaggcg cctgtaagag cccatccaaa accaaagagt
     9001 aaacctccgt agatgtttcc tttattcagt tttttatcta caaattcaat gggttcgtta
     9061 tagattgttt taatcttgaa ttttttaata ataaagacag acagcatacc tactgccact
     9121 gcagagccaa tcactccgta catatgaaaa ctctgcaacc tgaacatttc ctgtatacga
     9181 aaccaggaaa tcacttctgc ttttaccagg ataactccaa ataacagccc ggcaatggca
     9241 tatttaaaat tataccacca ggggtgattt agttcagatt cgttgaaaca aacagcatcc
     9301 agcgaacgga cttcgaagtc agtgttttct gttagaaatt cgtggtgctt tttttcgctg
     9361 atgggttctt gtgtatttaa attcataacg cctgattata attttagtga aaatggaagt
     9421 accagattgg caacgatgaa tccaccggcc ataaaactga tagttgtgat caaagagggg
     9481 aattgaagat tgctcaagcc catgattgca tggccgctcg tacatccccc tgcatagcgg
     9541 gttccgaaac caaccaataa gccgccacct accatcataa taattcctct tgcagtcagt
     9601 aactctggcc aggcaaataa atctttaggt accaatcctt cataattagt aactccgtag
     9661 gtaaccattt cctcagccag ctttgcattt acctgtactg gtcccgggtt agataatagt
     9721 tgtgcagcaa tcaaaccgcc cgctaaaatt ccggctacaa aaaacaggtt ccatatttct
     9781 ttcttccagt catatttgaa aaatggaata ttggctggta aacaggcagc acaggcatga
     9841 cgaagggatg aagaaattcc aaacgattta ttaccaacca gtaataaggc aggtactgtt
     9901 aacccgatca gggggccggc cacataccag ggccagggtt gtttaatcca ttccagcata
     9961 ttttttattt tttaagttag caaatgtcat ttagtaaata atgttactcc gtgatatttg
    10021 tcacagcgat taagttattt atttttttgt gagttcttct ttttttgcaa taattggttt
    10081 ataggggcca tacttgtgct gggtctcgtt aaacccatct gtataagggc caaaaggatg
    10141 atcgaatggc tttttagaaa tatccggcca atccttactc aggtcttttt tgggtcttgg
    10201 ctggctgtta acgtaagctg ccacatccca tgcttcttca tttgataatt tagggttgct
    10261 atgtgtggtc tggttaaatg gcatattgct tttgacatag cctgcaaagt tactcagccg
    10321 gtatagcccg gcaccatcat tgtaactatg cggtccccaa agtggagggt actcatagct
    10381 tataccattc aggcttaact ggccttctcc attagcacca tgacaacttt ggcattgagc
    10441 cgtataaatc cctttacctt taatagggtc agcggctcta tccatatata atattttctc
    10501 aataccgctg cctacaggtt tctcaccttt ttttacatct ttgcctaacc actttatata
    10561 agaataaatt gctttgtatt ctctactggt actgtcagga gctgttccat tcaaacttct
    10621 ttcaaaacag tcgcttactc ttttgtagac ggtttctgta gctccgctac gatccctgaa
    10681 ctttggatag gatgaaaata ccacgctgta attattaccc caactttttt tgcctgcaat
    10741 cagatgacag ttctggcagt tcattccgtt ggttattggc gctacagaac catgcgggcc
    10801 aaaatacctt gaagtatttg cgatgatatc ttcaccataa attaccattt ccctttcctc
    10861 accttccagc aggttgtcgg taaataaact gggtggaatc caggtactat cttcaggcat
    10921 tgcagtgaaa gcctgtttgc ctgaaggctt aattgctttc attatttctg tcaatgctac
    10981 aataactatt acaataatta ttattatcaa ttgccagttt gatttaatat gcttccacat
    11041 gttctattaa tttaaagctt atctgaattc ataggtagtt ccctgtaatt gtgtaagcag
    11101 tgtatgtggc tggtcggttg ctgtggctct cctctccagt ttgggagata ccggtgagtg
    11161 agctgcaagg tattcttcta tcacgtcatg cattaataca cccgaccgtt tcggattttt
    11221 aactttttct atccggcata atgtatcatc cggatcgcct tctctttcac aggctacaat
    11281 actgtattct ttattaatgt ctaaaggttc gccttttact tttatccaat tcactctttt
    11341 gcccaattca ttaccaatag taaagttcac ttccataccg gcaaaacgta caaaccaacc
    11401 gccaaatcgt ttagaagggt ctttggcaaa ggcattctgc aattcttttt cctgccaatc
    11461 ccataattgc ttaccagtga tgatccctaa ttttgcttca ctgtcaactg gcagcatgtt
    11521 ccataaaaat tcccttgtaa tatctgcttt acccttttta gggtcaacgg ctaaaggttg
    11581 gcaaaaacga aacccattac ttagcgcaat atctgttttg aatttccagt gtattgcatc
    11641 ggtaatcagg ttgtccattg gcgtttccat tacaaaatat cttactaatg gggtagtggt
    11701 ggtgccaatc actttggaaa gatcctggtg aaaaggagct gaagccttat ctaccttaga
    11761 ctgtagcttt ttgtctgcag gatattttgc aggatcaaca tcgagcaatt ggtatttata
    11821 atttttaatt cttccattct ccacttctat atccagcttg ccaaggaaag aaccaaaagc
    11881 gccgcactct actactttac tgtattttcc ttcaatgggc actcttactc tctcatgtgt
    11941 atcagcaccc aaaataaagt cagctcctgc tacagcagga ttattggcca atcccacctg
    12001 ctgtgccagt cccatatgcg ttactatgaa gatgattcca catccttcca cttcttttag
    12061 cagctttata tattttgcaa cattaaaatc ggcatgttca aaacggatgc cttcgctgta
    12121 agcaggagac tggcgttttg ggatgagatg atcggtatac ccgataaatc caactttagt
    12181 tccggcaata tatttaaccc aatagggtgg atagatcaat tcgccattat ctgcatcagt
    12241 tgttttgtgc cacatattgg cacagatctt agccgcattg gcatgcccca tatcatagag
    12301 cattttcttt ttcttataca caacttccca gtttcccgga agtatcaggt cgtaattgaa
    12361 gtcattcagt aagggtatca atgcttcgcc ttctgtcaat gaagcaacag cactaccgtg
    12421 gaaataatca cctccatcaa tcagtaatgt attggcggga ttttgttttc tgaaatgatt
    12481 gatcattgtc ttcagaacag ccagtccacc tctttttttg tatacggctt tcccattttc
    12541 ccaaaaaaat tcatcatgag tgtgcaactg ggcatggatg tctgatgtat acaaaatcgt
    12601 aaaacgggtg gaagtttcag gagtaagttg tttagtttcg ctgtttgtaa gcggaatagc
    12661 tccggcaacc cccgccagcg aagtacctaa cccggcaagg gtagtcttta ttaaaaagtc
    12721 gcgacgtgaa ttcgggctat cattttttag caatgatgac atgggcaatc atttagaaag
    12781 tgagaaaata ataatataaa attgaatgct ttttttatcc tgctccgtga caacagtcac
    12841 actgaacggt ctgcacgtaa agtttaacga ccaggaagat ttttcagcat aaagtcccgg
    12901 atatttccac ccatgatctg gtaaatttct tctttattaa acccctcttt taataaagca
    12961 tttacaatta atggtaaacc agtaacatca aaatgagttg taatagcgcc atcaaaatca
    13021 gagccaagtg ctactttatc cacgccaatc ttatcagcaa catagcggat actttttgct
    13081 gtggcatctg catcagttcc acaaacagca gtttcccaga gaccaatacc taccaggcct
    13141 tttcttcttc caatttccat taaatgcgca tcagataaat tacgattatt atcacataca
    13201 ccttttacac ccgtatgtga aatcagcatc gggctttgtg tcaatacaaa aatatcatca
    13261 atggttttag gggatgcatg ggccaggtca atgaccatgt gaaggctatc cattttcctg
    13321 attaactgtt ttcccttctc ggtcaaacca cccttattaa ttccatgtgc tgaaccagcc
    13381 cagctgttgt caaagaaatg tgttggacct atatagcgta cgccggcatc ataaaataat
    13441 tgaagattat tgatatcatc accaaggcaa tgtgcacctt caatgcccaa catgccggct
    13501 gtaagttttt tattaattct cctgtctgta atgtattgct gtaattcttc ctggttggtg
    13561 atgaccctga attccccatt tgagctcttt gctgatttat ataactccct gcattgatta
    13621 agtgcccttg attttacact aaaccagtct ttgggatttc gcagttgggc aaaactcaat
    13681 aatgcaatct gatccgtctt atcatcgttt ttttcaatat ttagtccttt cggtgtttta
    13741 ctgacgattg taaaaacctg gaaagccata ttggcttcct gcatacgggg aatatccaca
    13801 tgaccataac tatggtgctt caggttgttt ctgtcccata gcaaggcatc acaatgaagg
    13861 tccgcgataa atgggattga atcataccag tttacttttt gtacattgac aactttcagc
    13921 gtcactttat tttttgactt gtcaatataa gagggcgcaa atgcgaaaaa agctatcgta
    13981 gtaatgataa ggagaatggc aaaatacttt agaaattttc taaacatttt tactggtaat
    14041 ttactctttt ttgcaggact ggaatgtttt ttgacccgga tcagaattgg tagttaaaaa
    14101 ccaaattcca cagactcata tctactttgt tgatgacata tttatcatta ccataggaat
    14161 tgcctgtctt ttctttaaat acatacagta attgtgtttc taatcctttt aataagccgg
    14221 caaatttgta gcggatatca atattcaatt gattatagga tggaattccg tacttattca
    14281 atgaaaattc agttacttcc ggaagattat aattaccgaa tgcaacagaa gtagtgatcc
    14341 ttgcttttga tattttataa tttacttttc caactattgc atgtacatct cctaaccctt
    14401 cattacgttc tctgggaaga aatgtaaaga atgggtcacg gccccattct cttggcatca
    14461 ggtattgtcc gtgtgcagtg atcctgttat agttaatagt tgtttcccag ttaacatttt
    14521 tccagccaag ttttgttcca aaggtgaaag ctttagctcc ttttttgtag taagccttag
    14581 caggatcttc attaccgccg tcattgatgg cgtcctgccg tattacctgt gcagctacta
    14641 atagctgtga tttaccgtcc agcggatatt cataatctgc ctgcagaaaa gtgctgttaa
    14701 atatattttc agtgaactgg ttccaaagct gcagtttaag atttttattg tgttgataag
    14761 taattccggt aagaattatt cctttacttt ccaggttgcc ctggtaacct gattttattc
    14821 cattttcgtt aacaccaaca gggtacacac cgattgattc gttcacttta aaccatttca
    14881 cagtactgcg gggagaaatg ccatagaggt agcctaattg aagtttgatt ttttttattc
    14941 cgttaatttc cgcccataag ccttctactt cagttggccg catgcgacca tcctgcaggt
    15001 tgatgaaagg ggtattaatt aactgcttgc ctaatgtcag ggctgacttt ttgaagtggt
    15061 atttgatata caattcttcc agccggtcaa tatctttttt attggccggg tcttcaatgt
    15121 cgaataaacc agtttcatac cggttcatct ggtttgtttt tggatctggt atcgtcaggt
    15181 ctgcggagcc gatattgtaa acaaagaacc cactaacacc caattgaaaa tttttaaatt
    15241 ttccagtttc aaatttaata ccaccgccaa ctgcatgggc ataataatcg gtgaggtcgg
    15301 ctttgttgtt agtggacata aaataataac gtaaatgtcc gcttaccgtt cctcttctga
    15361 atgcagagag gatggaagta gaatcggttt cactttcttt ttctttccaa agcccgggct
    15421 tttcatttac ttcctggtgc tgagctacag cagcagacat agttatggtg aaccccaaaa
    15481 aaataaatat ttttttcatc gttaattagc tctattgctt aaggatttat ggcaggcaat
    15541 acgttgctga ggagtgttgt cagaaaccaa ttttaatata gctccagcct tgttcctgtt
    15601 tacgtacaat ttctccaatg cccattttca caaaggtagc ttcagcaatt attgactctt
    15661 taggtacttt tctttccatc atggtgtttt cacaggcaat gaaatcgata cccatttttt
    15721 tgaaatgagt aatcctttct tgctgagtcg ttttaccttt ggtcaggaga tcaataccgg
    15781 ggccatgcgc cactacttcg atgatcagac tgtcacccca gccggctttc agatgtttga
    15841 ggttattcat cagtcctttc catacaaggg tgtcattgct gctgagttgc attatgatac
    15901 ggtgttggga agtagttgtt gaaatatttt gtgcctgtag ttgtgtgaca aacagcgtta
    15961 tgacaacggc aataaaaaat ttcctataca tatgtttgtt atttgtgttg ggattactgt
    16021 tcttttactc cttcccagct gttaaaacca ccttgcagat cgaataattt agtaaaaccc
    16081 atttctttca tcactgtcat tgcatcttta ctccttttac cactacggca atacaagagg
    16141 taaggtttac ttttatccag tacagccacc tgttttttaa aatcttcagt ttttaaaaaa
    16201 tcaatttgta atgaaccagg gatatgccct gccttatatt caacttcagt ccttacatcc
    16261 agcagtattg tattcttctt gtttatcagt cgctgaaatt tattctggct aacagtttta
    16321 cctgcagtat tatcttgtgc ttgtacacaa accgtgctac atagacccgc cagtaataaa
    16381 ccgcctgcaa ttatcagtaa gctctttttt ttgatatttg ggagcaataa ggccatttca
    16441 aaatatttaa tttaattttc cagctgcaca actaataaat gatttggctt tagacatcag
    16501 ggtattgttt cctttctccg ggtaacccat ggctgccagt ttttcaatta gaaggccacg
    16561 atttattccg acacccatta tacaaatctt ttcttcttcc ttatacactt caacttcctg
    16621 aatgcctgcc atttttttca gtgcatcttt gatgcttttc
//
LOCUS       DAORTI010000073        16608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3190_length_16608_cov_7.435752, whole genome shotgun sequence.
ACCESSION   DAORTI010000073 DAORTI010000000
VERSION     DAORTI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3190_length_16608_cov_7.435752"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLO70_10195"
     CDS             complement(<1..286)
                     /locus_tag="PLO70_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74881.1"
                     /translation="MATQKLQQRSTFKKILRFFFQGLIILAPIGITLYAMYWLFERVD
                     GILRPYVNIPGLGFLLIILFVILVGWISSNFLMGSAITFFDQLMEKTPGVK"
     gene            427..1065
                     /locus_tag="PLO70_10200"
     CDS             427..1065
                     /locus_tag="PLO70_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQZ74882.1"
                     /translation="MEPKERILVKAEELFMQYGIRSVSMDDIANNLGMSKKTLYQYFA
                     DKDELVEAVVDGHIKGIQSDCESCRLEASDAIHEIFITMERIMEEFSNMNPMLVHDLE
                     KFHFKAYQRFREHKDKFLLKIIRDNIEWGIKDELYRNDINLDVMSKYRIESMMIPFNV
                     AVFPPGKYNLAATSGMIIENFTYGLATIKGHKLIQKYNDQRKKNLSYEENRK"
     gene            1046..>1758
                     /locus_tag="PLO70_10205"
     CDS             1046..>1758
                     /locus_tag="PLO70_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ74883.1"
                     /translation="MKRTGNKIPKIIGRLLVLSLLPFASTGQDTVKITRHEFSIQQAI
                     DYANKNNVNVKNALLDIKLQEQVNREITSRAYPGINASLGTTYNPNVATQVLPNFISP
                     ATYQVLIDEGVKDGNGNVIQMPDDFGFVAAQFGTKYSATAGISLSQILFDGQVFVGLQ
                     ARDAAISFYKKNAEITEEMIKTNIYKIYYQLLVSKTQVELIDANIAFLEKNLKDTRII
                     YENGFREKLDVDKVSVQLT"
     assembly_gap    1759..1858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1859..2628
                     /locus_tag="PLO70_10210"
     CDS             <1859..2628
                     /locus_tag="PLO70_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ74884.1"
                     /translation="NGFREKLDVDKVSVQLTNLQTEKRKILNSISNGYYGLKVLMGMP
                     VKDELVLTDTITDEQIKDGMLDAAVYKYEDRKDYQYLQIAKQLNEYNVRRYKLSQIPT
                     VTLNGQYAKNAQRSKWNFFGKGDWFTISNVSLNMSIPIFNGFYTKSKIEQARIEVRKT
                     DNQVEALKLSIDNDVATAQNNFRSAIATMDFQKKNMELAENIYQQTKKKYEVGVGSQT
                     EINAAQTDLKTAQTNYVTALYDAIVARVDFLKATGKL"
     gene            2668..3882
                     /locus_tag="PLO70_10215"
     CDS             2668..3882
                     /locus_tag="PLO70_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ74885.1"
                     /translation="MNNILRISFVAIVTVSFISCGTGAKDKKGEIGDLKVKIEKLKKE
                     KSSLDEDIRKLEDQLAKADPSAAKLVSRLVSVDTLRIGDFAHYIELQGKIDAEGMAYV
                     GPTGMGGLVKAVYVKLGQKVGKGQLILKLDDALARQQLEAARQQTGQLKARLAQAQTI
                     YERQQNLWKQNIGAEIQVINAKADVDALSAQLRAAQAQVGMAQEQVNMSNVYAQISGV
                     IDEMNVTPGEFFSPQSAATPGAGIRIVNNSNLKIVTNVPENYVSRVKKGDKVEIVVTE
                     TGKPAFQSTISVVGASIDPTTRAFTTEAKLPSDPLLKPNQTAVMKILDYQSKAAIAVP
                     VNIVQSDEKSKYVYVMEKSGDKMVARKKTVVAGEVYNGLMEIKSGLSGGELIITEGYQ
                     SVYDGQTITTGK"
     gene            3915..7478
                     /locus_tag="PLO70_10220"
     CDS             3915..7478
                     /locus_tag="PLO70_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQZ74886.1"
                     /translation="MKIVEGVVKKFKEFKPTSWAIDNKTAIYIIAILITAYGYMKFNS
                     LPKEQFPDIVVPTISITTVYVGNSPKDIENLVTRPIEKQLKGISGARVKKIQSTSQTD
                     YSLIVIEFDTDIKTDLAKQKVKDAVDKAKTDLPTDLTSQPDVQEFAFSEMPIMYVNVS
                     GDYDGIKLKEYADKMQDHFEELPEITRAEIVGAPEREIQVNVDPYKMTAAKVSFTDIE
                     NAISRENTDITGGLIEVGNMKRTLRVKGQFTSVLDLQNIVVRSSARGASVYLRDIAEL
                     KDTIKDKDSYARLDGKNVVTLNIVKRSGANLIDAADNVKATVAEMQENEEIPKDLKVV
                     ITGDQSRQTKTSFNELINTIIIGFILVLIILMFFMGVTNAFFVALSVPLSVFVAFLFL
                     PLADAVVGGSVTLNFIVLFALLFGLGIIVDDAIVVIENTHRIFDNGKVPIVRSAKEAA
                     GEVFIPVLAGTATTLAPFFPLLFWKGLIGKFMIYLPTILILTLAASLIVAFFFNPVFA
                     VSFMKPEGRQFDKPKTAVFRSKWFIVFTIAGVLLHLPGMHGVANFSLLMAILMVFNSY
                     VLNDVIHAFQQKVLPGLMNRYEKLLRWVLKGRRPIWAFISLFGLFIIAVAMLLLRGNK
                     TTFFPSGDPNFIYVYLKLPVGTDVKYTDSITRELEKRVYKVLEKEKPGTEGSIVESII
                     TNVAVSANNPRDNNRSTQPNLGRIQVSFVEFEKRHGKETMPYKDAIREAVQGIPGTNI
                     EVAQEDGGPPTDPPVNIEIAGDNFESLSEVATNLLNYLDTNRVEGIENLQADIDLNSP
                     EISVNVDRERAMMEGLSTGQIGMAIRTAVFGKEVSKLKDGEDEYKIQLRYTDLRRNNV
                     TDLMNMNITFMDMNTMQVKSIPLSSVAKVDYTNTTGGVKRKNVKRTIQLQSNVLDPTM
                     VAPINAALKNKIDDFKGKVRIPADVTIRQSGQSEQEQETQQFLGMALLIALGIIFLIL
                     VLQFNSMSKPFIVITEIFFSVIGVLLGYALTGMTIATIMMGVGIVGLAGIVIKNGILL
                     IEFTDELRSRGYRTREAAIQAGKIRIIPVLLTAVATILGMFPLAVGFNIDFAGLFQHL
                     RPNIFFGGDSVVFWGPLAWTIIFGLIFSFFLTLIMVPSMYLMAERLKRPMEKFYGTKF
                     IALLGFAGPFFFIFVGIMFGVRRLQGKKVWLGKPKLVTKNA"
     gene            complement(7566..10421)
                     /locus_tag="PLO70_10225"
     CDS             complement(7566..10421)
                     /locus_tag="PLO70_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQZ74887.1"
                     /translation="MKQRFLLLSLFSLFIATIVNGQAKLVEKVSKKGNELVITYEKYV
                     LPNGLTLIIHEDHSDPVVHVDVTYHVGSAREEIGKSGFAHFFEHMMFQGSDNVADEQH
                     FKIVSDAGGTLNGTTNRDRTNYFETVPSNQLEKMLWLEADRMGFLLDAVTQKKFEIQR
                     STVKNERGQNYDNRPYGLAAEAGAKNLYPYGHPYSWLTIGYVEDLNRVNVNDLKNFFL
                     RWYGPNNATVTIGGDVKPAEVVKLVEKYFGVIPAGPEVKPVVVPPAVLEKDRYVSYTD
                     NYARLPLLTIAYPTVPNYHKDMAALACLAQVLGQGRNSTLFQELVKTQKALQASAFSQ
                     LSELAGEFSFRITPSPGKSLADMDSLYRAALKTFAATGVTDDDIAKFKGGIESQLING
                     LQSVSGKVSQLASFQTFTGNPNKIAELIKMYQAVTKEDVMRVFNEYINNKHAVYLSVL
                     PKGQEKLIAAADNYTIDSSRYTAPEYGYAGLKYTKAKDNFDRSKIPGNGPNPIVKVPK
                     FWRKDMANGVRMIGTESTEVPTVTITITIPGGHILQANDTAKIGLADMFGDMMNEDTK
                     NYTAEQMSIALQKLGSSVNVGSSFNGITISIQSLKKNLDATMTLVEERILNPKFTEAA
                     FSRIQKQNLESFKQTRADPAAVANNVFAKVNYGPDNILAMSESGTEHTVRNLKVKDIQ
                     DYYDNYITSQGTKVVVVGDVKQEEILPKLAFLNKLPNKKVTLPKVNSTPPAIDKTKVF
                     LVDIPKAAQSQFRVGYATGLKYDATGNYYRAILTNYALGGDFNSRLNLNLREDKGWTY
                     GARSGFSGDEYSGDFAFSSGIRADATDSALVEVMKEINNYRTNGITEAELKFMKNSLG
                     QRDARNYETGIQKAGFIGRILNYNLPADYVDQQNKILAKLTKQEVDAVAKKMLNPDKM
                     NILLVGDKAKILESVRKLGYEVIELDAEGKKMEKKGF"
     gene            10606..11409
                     /locus_tag="PLO70_10230"
     CDS             10606..11409
                     /locus_tag="PLO70_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HQZ74888.1"
                     /translation="MSTAPRVNHEIDYTIYGEEMQFVEIELDPAETAVAESGSMMMMD
                     DGIQMQTIFGDGSAQQPAGFLGKLMSAGKRILTGESLFMTAFTNAVQGKRKVSFAAPY
                     TGKIITLDLQQLGGTIIAQKDAFLCAAKGVSVGIHFQRKLGTGLFGGEGFIMQKLEGD
                     GFAFLHAGGFIVERELKPGEVVKIDTGCVVAYTPSVDFDIEFIRGIKNFMFGGEGLFF
                     ARLQGPGKVWIQSLPISRLAGRIMQYGTYNRKEEGSVLGGLGNLFDGRE"
     gene            complement(11481..12410)
                     /locus_tag="PLO70_10235"
     CDS             complement(11481..12410)
                     /locus_tag="PLO70_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74889.1"
                     /translation="MGQLGIILLSLLLLATSRNYAQGCSDAGVCTIDAIKQNTIPGRD
                     IANTNNWLSAGTGYAKGERGTNIYTMQLEYTRRVAANLFVTGKLSYNYINGELGNSSG
                     PGDLFLTIEKKLKATTTWKKSVFTGLKIPFTSANKKINELDLPMVYQPGLGTTDLLLG
                     ITASTRFIGFSLAFQQPLSGSNNNNFLPSLYPLHSDAKNYPATNKFSRKADIVARISR
                     NFNAEKKLSISPGLLGILHTGNDSYIDENNNRQVIEGSGGFTLNATTFLQYAANKKNS
                     IGLSAGIPLIVRAERPDGLTRKFVLALEYSFNF"
     gene            complement(12488..13246)
                     /locus_tag="PLO70_10240"
     CDS             complement(12488..13246)
                     /locus_tag="PLO70_10240"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQZ74890.1"
                     /translation="MDIKEYISSGIIELYVMGLCSPEEEKELEALRPQYKELHEAILQ
                     YEKDMEEKMLRYATLPDAETDKQILQQLASLNTPVVQMRPVQSTGLKFNWMRSVAAAA
                     VILLGISAYFNYTLYNKTKKQAALISNSTSSPLPAADYTVITNPAITPVALYGVGSHA
                     ICRCTMFWDKSTGKAYIMIHHLPRSSSSKDYQLWAEVDGKTVNVGIINDAIRGRFIEV
                     TNVPNQSIAFKVTLEQSGGAAQPTLAETYLEGKI"
     gene            complement(13250..13819)
                     /locus_tag="PLO70_10245"
     CDS             complement(13250..13819)
                     /locus_tag="PLO70_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ74891.1"
                     /translation="MIFTFQFSKLEQKPYTEAELVTALKNRDNHAYKHLYLHYRGALY
                     NNILQLLPDREIAADVLQEVFLQIWKQIDKYDETKGRLFTWMLKLTRNMAINQTRVKN
                     FKVHSKNEDISNYVSIIEERNNQQTGINHIGLRQQVHKLRPEYKDVIELSYFQGFKQE
                     EIAEALKLPLGTVKTRLRNAVIELRKEFK"
     gene            14178..15365
                     /locus_tag="PLO70_10250"
     CDS             14178..15365
                     /locus_tag="PLO70_10250"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HQZ74892.1"
                     /translation="MRIIQLMAYTTIAVVLLASCQKEKSRLENNFNEDENSVTANKSS
                     GEDESISQRGNNRGFVYTLSNETSGNKVITYKRAANGSLTYIASYASGGNGSGGGLGN
                     QGAVIISNDGNVLLAVNAGSNTISSFKITGNGLNLKSTVSSGGITPVSIAQHNDLVYV
                     LNAGGNGNISGFRLGENDKLTPLSSSTRPLSSSMAGAAQVSFVRNGKVLVITEKATNK
                     IITYTVNASGRPEAMHSITAASPTPFGFAPGRFGNIYVSEAVGGAPNASNVSSYHISY
                     NGSISLSEGPVATGQSAACWVVLTENGRYIYTTNTASNNLSSYTVHPFNGNINLFQSA
                     AATSGMGPIDAALTDNSKYLYVLNAGSHSITGYSVAGNGTLTVLPGTITGLPASTNGL
                     AAD"
     gene            15444..15854
                     /locus_tag="PLO70_10255"
     CDS             15444..15854
                     /locus_tag="PLO70_10255"
                     /inference="COORDINATES: protein motif:HMM:NF021996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HQZ74893.1"
                     /translation="MKNIIIASAMLMALISSCKKNVPDNIQEQVPRGTVLKSGTFVSN
                     GKTTSGTARIVAGENNTVKLVFENFSTGNGPDVRVWLSPNTTATTYLELGALKAVNGT
                     FSYDLNSSIDYTVNNRVLIWCEDVNLLFGHAVLQ"
     gene            complement(15898..>16608)
                     /locus_tag="PLO70_10260"
     CDS             complement(15898..>16608)
                     /locus_tag="PLO70_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ74894.1"
                     /translation="QQQKKADSIAKAAERKKKIYITFDDGPNKGTRYVLAAVKEENIA
                     ASFFIVGVHIFDSPRQKETWEMLKSDTTIERCNHSYTHAHNRYTSFYEQPGKVVDDFK
                     QAQKIAGFSNSIARMPGRNAWRIDTIDHTDIYESKTAIDSLHQAGFAIMGWDIEWMFD
                     HKTLAVDTATGLLLRRIDNMLAAGKTKTPGHLVLLAHDQAFQKTEDIEKLHYVIQQLK
                     NNPDYELLFASKYPGVKH"
BASE COUNT         4894 a         3173 c         3671 g         4770 t
ORIGIN      
        1 atttaactcc cggtgttttt tccatcagtt ggtcaaaaaa ggtgattgca ctgcccatca
       61 ggaaattgga acttatccag cctacgagga tcacaaataa aatgatcagg aggaagccca
      121 gaccaggaat gtttacataa ggccgtaaaa tgccgtctac cctctcaaac agccaataca
      181 tggcataaag agtaatgcca attggtgcca ggatgattaa cccctggaaa aaaaagcgta
      241 gtattttttt gaatgtactc ctctgctgca gcttttgggt agccatattc ctgatttaca
      301 ggctaaggta ctgcaaaata aacctcaatg accggttcaa aaagtcggtg aacggtcaaa
      361 aaagcaggat ggaaactttg gttacgaaaa aagtttccat agttttgctc ttcgaaatca
      421 gtgaatatgg aacccaaaga acgaatatta gttaaggcgg aagagctgtt catgcaatat
      481 ggtatccggt ccgtatcgat ggatgatatt gcaaacaacc tgggaatgag taaaaagaca
      541 ctctatcagt attttgctga taaagatgag ttggtggaag ctgtagtgga cggccatatt
      601 aaaggtatac agtcagattg tgaaagctgt cgcctggagg cctccgatgc cattcatgaa
      661 attttcataa cgatggaaag aatcatggaa gagttcagta atatgaaccc catgctggtg
      721 catgacctgg agaaatttca tttcaaagcc taccagcggt tcagggagca taaagataaa
      781 ttcctgctga aaattatccg ggataatatt gaatggggta taaaagatga gttatacaga
      841 aatgatataa acctggatgt aatgtcgaaa taccggatag aatcgatgat gatcccattc
      901 aatgtggcag tttttccccc gggcaaatat aacctggctg caacttccgg aatgatcatt
      961 gaaaatttta cttacggatt agctaccatc aaaggacata aactgataca gaaatacaat
     1021 gaccaacgta aaaaaaacct ttcgtatgaa gagaacagga aataagatcc cgaaaattat
     1081 cgggagacta ttagtattgt cattattacc atttgcatca acagggcagg atacggtaaa
     1141 aatcacccgt catgaatttt ccattcagca ggccatcgat tatgcaaata agaataatgt
     1201 gaatgtgaaa aatgccttgc tggatataaa attgcaggaa caggtaaaca gggaaataac
     1261 gtcgagagct tacccgggca tcaatgcaag tttagggact acttataacc ccaatgttgc
     1321 cacacaggtg ttgcccaatt ttatttcgcc ggccacttac caggtattga tagatgaagg
     1381 ggtaaaggat ggtaatggca acgtaataca aatgcccgac gattttggat ttgtcgcagc
     1441 acagtttggt acaaagtaca gtgctacagc gggtataagt ctttcacaaa ttttatttga
     1501 tggccaggtt ttcgtaggac tgcaggcaag agatgctgcc attagttttt ataagaaaaa
     1561 tgccgagata acggaggaga tgataaaaac taatatttat aagatctatt accagttact
     1621 ggttagtaaa acacaggtag aattaattga tgccaacatt gcctttctcg aaaaaaacct
     1681 gaaagacacc cggataattt atgaaaatgg attccgggaa aagctggatg tagataaagt
     1741 aagtgtacag ttaaccaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1861 aatggattcc gggaaaagct ggatgtagat aaagtaagtg tacagttaac caatctgcag
     1921 accgagaaga gaaagatttt gaatagtata tcgaatggat actacgggct aaaagtatta
     1981 atgggtatgc ctgtaaaaga cgaactggtc ctgacagata caataacaga tgaacagatt
     2041 aaagatggta tgcttgacgc agctgtctat aaatatgaag acaggaaaga ttatcaatac
     2101 ctgcagattg ctaaacagtt gaatgagtat aatgtacgcc ggtataaatt aagccagatt
     2161 cccactgtca ccctgaacgg tcaatatgcc aagaatgcac aacgcagcaa atggaatttt
     2221 tttggtaaag gggattggtt tactatttct aatgttagtc tgaatatgtc catacccatc
     2281 tttaatggat tttataccaa atcaaaaatt gaacaggccc gcatcgaagt gagaaaaact
     2341 gataaccagg ttgaagcttt aaaattatcc atcgataatg atgtggctac agcacaaaat
     2401 aatttccgtt ctgctatcgc tactatggac tttcagaaga agaatatgga gctggcggaa
     2461 aacatttatc agcaaaccaa aaagaagtat gaagtagggg tcggctcaca gacggaaata
     2521 aatgcagcgc agacagatct gaaaacagca caaactaatt atgtaacagc tttatatgat
     2581 gccatagttg ccagggtcga tttcctgaaa gcaacaggca aattataaag attaaaaaac
     2641 caacacatgt cttataaact gaataatatg aataacattt taaggatttc ttttgtagct
     2701 atcgttacgg tttcattcat aagctgcgga accggtgcaa aagacaagaa aggagagata
     2761 ggtgatttaa aagtaaaaat cgaaaagctt aagaaagaga aaagcagttt ggatgaagat
     2821 atccgcaaac tggaagacca gcttgccaaa gctgatccct cagcggccaa actggtatca
     2881 aggcttgtct cagtagatac attacgtatc ggggattttg cgcattatat cgagctgcag
     2941 ggtaaaatag atgcagaagg gatggcttat gtaggaccaa caggaatggg ggggctggtt
     3001 aaagcagtat atgttaaact gggacagaaa gtaggaaaag gccaactgat cttaaagctt
     3061 gatgatgcgc ttgcccggca acaactggaa gcagccaggc aacaaaccgg gcagctgaaa
     3121 gcaagacttg cacaggctca gactatttat gaacgacagc agaacctatg gaagcaaaat
     3181 attggagcag agatccaggt tattaatgcg aaagcagatg tagatgcttt gtcggcacag
     3241 ctgcgggctg cacaggcaca agtggggatg gcgcaggagc aggttaatat gtcaaatgta
     3301 tatgcccaga taagcggtgt gatcgatgaa atgaatgtaa cacctggtga attcttttca
     3361 ccgcagagtg cagctacacc gggtgcaggt attcgtattg taaataacag taacctgaaa
     3421 attgttacta acgtacctga aaattatgtt tcgagagtga agaaaggaga taaagtggag
     3481 attgtggtta ctgaaacagg taaaccggct tttcaatcca ccatcagtgt agtaggtgca
     3541 tcaatagacc caactacaag ggcatttact acagaggcaa aacttccttc tgatccgtta
     3601 ttgaaaccca atcagaccgc ggtgatgaag atactggact accagtcaaa agctgcaata
     3661 gcggtgccgg tgaacattgt acagtctgat gagaaaagta agtatgtata tgtaatggaa
     3721 aaatctggtg ataagatggt ggccaggaag aaaacagtag tagctggtga agtgtataat
     3781 ggccttatgg aaattaaaag cggattgagt ggtggtgaac tgatcattac agaaggttac
     3841 cagtctgttt atgacgggca aactataaca actggtaaat aaacaacagt cgaaaagact
     3901 tcaaaaataa tagtatgaaa atagtagaag gggttgttaa aaaattcaaa gagtttaaac
     3961 cgaccagctg ggctattgat aacaaaacag cgatctatat cattgcaatt cttattaccg
     4021 cttacgggta catgaagttt aacagtttac ctaaagagca gtttcctgat attgttgttc
     4081 caactatctc cattactacc gtgtatgttg gtaattcacc aaaggatatt gagaatcttg
     4141 ttacccgccc gatagagaaa caattaaaag gaatcagcgg tgccagggtt aagaagatac
     4201 agagtacatc acaaacggat tatagcctga tcgtaattga atttgatacc gatattaaaa
     4261 cagatctggc caagcaaaaa gtaaaagatg ctgtagataa ggccaaaacg gatttgccga
     4321 ctgatttaac ttcccagccc gatgtacagg aatttgcttt cagcgaaatg cccattatgt
     4381 atgtaaatgt cagtggtgat tatgatggaa ttaagcttaa agaatatgcg gataagatgc
     4441 aggaccattt tgaagagttg ccagagatca ccagggcaga gatcgtgggt gcaccggaac
     4501 gggaaataca ggtgaatgtt gacccgtata aaatgacagc agcgaaggtc agtttcacag
     4561 atattgaaaa tgcgatcagc cgggagaaca ctgatataac cggtggattg attgaagtgg
     4621 gaaatatgaa gcgtaccctg cgggtaaaag ggcaatttac cagtgttctg gatttgcaga
     4681 atattgtagt gcgaagcagt gcccggggag cttcagttta cctgagggat attgcggaat
     4741 taaaagatac aataaaggat aaggatagtt atgcgaggct tgacggtaaa aatgtagtta
     4801 cactgaatat agttaaaaga tcaggtgcca atcttattga tgcagcagat aatgtaaaag
     4861 ctactgtagc agagatgcag gagaatgaag aaatccctaa agacctgaaa gtagtgatta
     4921 caggggatca aagccgccag acaaagacct catttaacga actgatcaat acgatcatta
     4981 tcggattcat cctggtattg atcatcctga tgttttttat gggagtaacg aatgcatttt
     5041 ttgtggcatt aagtgtgcca ttgagtgtgt ttgtggcttt cctctttttg cctcttgcgg
     5101 atgccgttgt aggagggagt gttacactga acttcattgt tctttttgcc ctgctgtttg
     5161 gattgggaat catcgtggat gatgcaattg tggtcattga aaatacacac cggatatttg
     5221 ataatggaaa agtaccaatc gtccggagtg ccaaagaagc ggcaggggag gtctttatac
     5281 ctgtactggc aggtacggcc accacgctgg ctccgttctt ccctttgtta ttctggaagg
     5341 gactgatcgg taagttcatg atctacctgc ccactattct tattcttaca ctggcagcat
     5401 cactgatcgt ggcattcttt ttcaacccgg tatttgctgt aagcttcatg aaaccggaag
     5461 gcaggcaatt tgacaaaccc aaaactgctg tgttcagaag taaatggttc atagtattta
     5521 ctattgctgg tgttttatta caccttccgg gtatgcatgg tgttgccaat ttctctttgc
     5581 tgatggcgat actgatggtg tttaattctt atgtactgaa tgatgtgatt catgcatttc
     5641 agcaaaaagt attaccgggt ttgatgaacc gctatgaaaa acttttacgc tgggtattaa
     5701 agggcaggcg gcctatctgg gctttcattt cactttttgg tctttttatc attgctgttg
     5761 ccatgttgtt gttaagaggc aataaaacga cctttttccc gagcggtgat cccaatttta
     5821 tctatgtata cctgaaactt cctgttggta cggatgtaaa atatacggac agtatcaccc
     5881 gtgaactgga aaagagagta tataaagtat tggaaaaaga gaaaccgggc acagaaggaa
     5941 gtattgtgga aagtattatc accaatgtgg cagtcagtgc caacaatcca agggataata
     6001 accgcagtac acagccaaac ttaggtcgta tccaggtatc ttttgtggag tttgaaaaac
     6061 gtcatggtaa agaaaccatg ccatataaag atgctatacg ggaggcagtg cagggaattc
     6121 cgggtacaaa tattgaagta gcacaggaag acggaggtcc tcccaccgat ccaccagtta
     6181 atattgaaat agccggagat aattttgaaa gcctttccga agtagccacc aatttgctga
     6241 attacctgga tacaaaccgg gttgaaggta ttgaaaacct gcaggccgat atagacctga
     6301 atagcccgga gatctctgtc aatgtagaca gggagagggc tatgatggaa gggttaagta
     6361 ctggccagat cggcatggcc atccgtactg cagtgttcgg taaagaagta agtaaactga
     6421 aagatgggga agatgagtac aagatacaac tgcgctatac ggacctgcgc cggaataatg
     6481 tcactgacct gatgaatatg aatatcactt ttatggatat gaataccatg caggtgaaat
     6541 ccatcccgct cagctctgtt gcaaaagttg attataccaa tacaaccgga ggtgttaaaa
     6601 gaaaaaatgt aaaacgtacc atacaattac aaagtaatgt gctggatcct actatggtag
     6661 cacccattaa cgcagcgttg aaaaataaaa ttgatgattt taagggaaaa gtaaggatcc
     6721 ccgctgatgt tacgatcagg cagagtggtc aaagcgaaca agaacaggaa acccagcaat
     6781 tcctgggtat ggctttactg atcgccctcg gcattatttt cctgatactg gtactgcagt
     6841 tcaatagtat gagtaaacca ttcattgtga ttacagaaat tttcttctct gtaatagggg
     6901 tattgttggg ttatgcatta accgggatga ccattgccac tatcatgatg ggagttggaa
     6961 ttgtaggact ggctggtatt gttattaaaa atggtatcct gctgatagaa tttaccgatg
     7021 aattgcgcag caggggttac agaaccaggg aagctgcgat acaggcaggg aagatcagga
     7081 tcattcctgt attgttaaca gcagtagcta cgatattggg tatgttcccg ctggcagtag
     7141 gatttaatat agattttgcc ggattattcc agcatttacg tcccaatatt ttctttggag
     7201 gtgatagtgt ggtgttctgg ggaccattag catggaccat tatcttcgga cttattttct
     7261 ctttctttct cactcttatc atggtgccaa gtatgtacct gatggcagaa aggctgaaaa
     7321 gaccaatgga gaaattttat ggtaccaagt ttattgcctt gttaggtttt gcaggcccat
     7381 tcttcttcat cttcgtcggg atcatgttcg gggtcaggcg gctgcagggt aagaaagtgt
     7441 ggttgggaaa gccaaaactg gtaactaaga atgcataata aatgaaaaac cgtggttggt
     7501 tttgtggtta aaaaaagcgg ctctctttcg agggccgctt tgcttatcag gtattgtttc
     7561 aatatttaga atcctttctt ctccatcttc tttccttctg catccagttc aattacttca
     7621 taaccaagtt tcctcacact ctccaggatc tttgctttat caccgacaag aagaatattc
     7681 attttatcgg ggttgagcat tttcttcgca acagcatcta cttcctgttt ggtcagttta
     7741 gccagtattt tattttgctg gtctacataa tcagcaggca ggttataatt gagaatacgg
     7801 ccaataaaac ctgctttttg tataccagtt tcatagttac gggcatcccg ctgacctaat
     7861 gaatttttca taaacttcaa ttcagcttcg gtaatgccat tggtgcggta attattgatt
     7921 tctttcatga cttccaccaa tgcactgtca gtagcatcgg ctctgatacc ggaactgaat
     7981 gcaaaatcac cactgtattc gtccccgcta aacccactgc gggcaccata ggtccagcct
     8041 ttatcttcac ggagatttaa attcaaccgg ctgttgaagt caccgcccaa tgcataattg
     8101 gttaatatag cacggtagta attgccggtg gcatcatatt tcagaccagt tgcataacct
     8161 acacggaatt gtgattgtgc cgctttcgga atatcaacta aaaacacttt tgttttatca
     8221 atagcaggag gggtgctgtt tacttttggt aaggttactt ttttgttggg cagtttattc
     8281 agaaaggcca gtttaggaag aatttcttct tgtttaacat cacccactac taccactttg
     8341 gttccttgag aggtaatata attatcataa tagtcctgta tgtcctttac tttcagattc
     8401 cttacagtgt gctcagttcc ggactcactc atggcaagaa tattatcggg cccataattt
     8461 actttggcaa atacattatt tgcaactgca gccgggtcag ccctcgtttg tttaaagctt
     8521 tcaaggtttt gtttctgtat ccggctaaaa gctgcttctg taaacttggg attcagtatt
     8581 ctttcttcta ctaaagtcat cgtagcatca aggttcttct ttaacgactg aatactgata
     8641 gtgatgccat taaagctgct gccgacatta acagaactgc ctaatttttg taaagcaatg
     8701 gacatctgtt cagccgtata atttttagta tcctcattca tcatgtcgcc aaacatatcc
     8761 gcaaggccga tttttgctgt atcatttgcc tgtaagatat gcccgccggg gatagtaatg
     8821 gtaatcgtga ctgtcggtac ttctgtgctt tctgttccga tcattcttac gccattggcc
     8881 atatcttttc tccagaactt tggcactttt acaatcgggt taggaccgtt gcccggtatt
     8941 ttgctgcggt caaaattatc ttttgctttc gtatacttta aaccggcata accatattcg
     9001 ggggcagtat atcgtgaaga atcaattgta taattatcag cagctgcaat cagtttctcc
     9061 tggccttttg gtaaaacact caggtaaact gcatgtttat tgttgatata ctcgttaaat
     9121 acccgcatca catcttcttt cgtaactgcc tggtacattt tgatcagctc agcaatttta
     9181 tttggattgc ctgtgaatgt ctggaaggaa gctaattgag aaactttgcc ggaaacactt
     9241 tgcaaaccgt taatgagttg tgattctatt ccacctttga atttggcaat atcatcatcc
     9301 gttacacctg ttgcagcaaa tgttttgagt gctgcccggt acaaactgtc catatctgcc
     9361 aatgatttac cgggtgatgg ggtaatacgg aatgaaaatt ccccggccaa ttctgacaac
     9421 tggctgaaag cactggcctg taaagctttt tgtgttttca ccagctcctg gaaaagcgtt
     9481 gaatttctgc cttgtcccaa tacctgtgca agacaagcca gggctgccat atctttatgg
     9541 taattgggaa ctgttggata agcaattgtc agcaacggaa gacgggcata attatctgta
     9601 taagaaacat agcgatcctt ttcgagtaca gccggcggta ctactacagg ttttacttcc
     9661 ggaccggcgg gtataactcc aaaatatttt tctaccagtt ttactacttc ggctggtttt
     9721 acatcacccc cgattgtaac agtggcatta ttaggtccat accatcttaa aaagaagttc
     9781 ttcaggtcat ttacatttac ccggttcagg tcctccacat aaccaatagt cagccaggag
     9841 taggggtgac catatgggta cagatttttt gcaccggctt ctgctgctaa tccataagga
     9901 cggttatcat aattctgtcc tctttcattc ttaacggttg aacgctgtat ctcaaatttc
     9961 ttttgtgtta cagcatccag cagaaagccc atacggtctg cttccagcca tagcattttc
    10021 tctaactggt tgctgggtac tgtttcaaaa taattggtgc ggtcacggtt ggttgttcca
    10081 ttcaacgtac caccggcatc tgatacgatt ttaaaatgtt gttcatcggc tacattatca
    10141 cttccctgga acatcatatg ctcaaagaaa tgcgcaaatc ctgacttgcc gatctcttct
    10201 ctcgctgagc ccacatggta ggttacgtcc acatgcacca cagggtcact gtggtcttca
    10261 tggataatga gggtaaggcc attgggcagc acatattttt cataagtgat aaccaattcg
    10321 tttccctttt tggaaacttt ttccaccagc tttgcctgtc catttacaat agtggctatg
    10381 aatagtgaaa aaagacttaa caataaaaaa cgctgtttca taaataaatt gattttaagg
    10441 gaaaacaaag tacggcaatc cgcggttcaa accagcattt tttaacattc cgggctataa
    10501 ccagggattc tcattctgct gctgtaaaag gagctgtttc ttccaaaatg gaaggaagtt
    10561 tacgctggta acattcctga ttttaacttt gcaaaaaatt aaaatatgag tacagcaccc
    10621 cgtgtcaacc atgagattga ctatacaatt tatggagaag aaatgcagtt tgtggaaatt
    10681 gaactggatc ccgctgaaac tgctgtggca gaaagtggat cgatgatgat gatggacgat
    10741 ggtatccaga tgcaaacgat ctttggggat ggctctgcac aacagccggc cggcttcctg
    10801 ggaaaattga tgagtgctgg caaaagaata ctgaccggtg aaagtttgtt catgacagct
    10861 tttaccaatg ctgtacaggg taaacggaaa gtaagttttg ctgctcctta taccggaaag
    10921 atcattacac tggatcttca gcaactggga ggaaccatca ttgcgcagaa agacgctttt
    10981 ctttgtgccg ccaaaggcgt cagtgtcggc attcactttc agcgaaaact gggaacgggt
    11041 ttgtttggcg gagagggatt tattatgcaa aaactggagg gagatggttt tgcttttctg
    11101 catgccggcg ggtttatcgt ggaaagagaa ttaaaacccg gtgaagtagt aaagatcgac
    11161 acgggttgcg tagtagccta tacaccttct gttgattttg atatagaatt tataagaggg
    11221 atcaagaact ttatgtttgg cggtgaaggt ctgttctttg ccaggttgca ggggccggga
    11281 aaggtttgga ttcaatcatt accgatcagt cgtcttgccg gccgtattat gcagtatggt
    11341 acttacaacc gtaaagaaga agggagtgtg ctgggtggat taggtaattt gtttgacgga
    11401 agagagtaac acaggatctt tatatttata taatgaataa taggggtaaa atcctgttag
    11461 taataagcaa aatgaataca ttaaaaattg aaggaatact caagggccag tacaaatttc
    11521 cttgttaatc cgtccggtcg ttctgctctc actatcaaag gaatacctgc agataacccg
    11581 atactgtttt ttttatttgc agcatactgc aggaaagtgg tggcatttaa tgtgaatccg
    11641 ccagatccct caataacctg ccggttatta ttttcatcta tataactgtc attgccggta
    11701 tgtaatatgc ccagcagccc cggcgaaatg gataattttt tttctgcatt aaaattccgt
    11761 gagatgcggg ctacaatatc cgccttcctt gaaaatttgt tagtagcagg gtaattcttt
    11821 gcgtcactat gcaaaggata cagcgagggt aaaaaattat tattgtttga tccggatagt
    11881 ggttgctgga atgctagcga aaaaccaatg aagcgggtag aagcagtaat acctaataaa
    11941 agatcagtgg ttccaagtcc cggttgataa accatcggaa gatccaactc attaattttt
    12001 ttattagcgc tggtgaatgg aatttttaaa ccggtgaaaa ctgatttttt ccatgttgta
    12061 gttgctttta attttttttc aatggtcaaa aacaaatcac caggaccgct gctgttaccc
    12121 agttcaccat tgatatagtt ataactgagc tttcctgtca cgaataaatt tgcagcaaca
    12181 cgacgggtat attccagttg cattgtatag atatttgttc cccgttcacc ttttgcataa
    12241 ccggtgcccg cactcagcca gttatttgta ttagctatat cccgtccggg aatagtgttc
    12301 tgttttattg catctatagt acacacccca gcatcactgc atccctgtgc ataatttcta
    12361 ctggttgcta aaagaagtaa ggataatagt atgattccga gctggcccat atatgctgat
    12421 tttaaacaga tatacagagc taatggaaag aaagttttcc gaacgtaaac attttcaaat
    12481 aaaagggtta gatctttcct tccaggtaag tctctgcaag ggtaggttgg gcggcacccc
    12541 cagactgttc aagggtaact ttaaatgcaa ttgattggtt aggaacattg gtcacttcta
    12601 taaaccggcc cctgatagca tcattgataa taccaacgtt tacagtttta ccatccactt
    12661 cggcccataa ctggtagtct tttgaagaag aagaccgggg aagatgatgg atcatgatat
    12721 aggctttacc ggtgcttttg tcccaaaaca tggtacagcg gcaaatagca tgagaaccta
    12781 caccatataa cgcaaccggt gtaatagcag ggtttgtgat aacggtatag tcggctgcag
    12841 gtagcggaga actggtggag ttacttatta atgcagcttg tttttttgtt ttattgtata
    12901 aagtgtaatt gaagtaagcg cttatgccca ataaaattac agctgctgca gcaaccgatc
    12961 tcatccagtt aaacttaagg cccgttgatt gtacaggtct catctgaaca acaggggtat
    13021 ttaacgatgc aagctgctgt aaaatctgtt tgtctgtttc cgcatcaggt aaagtagcat
    13081 accgaagcat tttttcttcc atatcttttt catactgaag gatagcttca tgcagttctt
    13141 tgtattgagg acgaagtgct tcaagctctt tctcttcttc cggagagcaa agacccatca
    13201 catacaattc gatgataccg gatgatatat attctttaat atccattaac tatttaaact
    13261 cttttctcag ttcgataact gcatttcgta accttgtttt aactgtgccc aatggtaatt
    13321 taagtgcttc agcaatttct tcctgcttaa atccctgaaa ataggaaagt tcaatcacat
    13381 ccttatactc gggccgcaat ttgtgaacct gctggcgaag cccgatatga tttatcccgg
    13441 tctgctggtt attcctttcc tcaattatag aaacgtaatt actgatgtct tcgttttttg
    13501 aatggacttt aaaattttta acccttgtct ggtttatagc catgttacgg gttagtttca
    13561 gcatccaggt aaaaagcctt ccctttgttt cgtcatactt atcaatttgt ttccagatct
    13621 gcagaaacac ttcctgcaat acatccgcag ctatttccct gtccggaagc aactgaagga
    13681 tattattata caaagcacca cggtaatgca ggtacaggtg cttgtatgca tgattgtcgc
    13741 ggttttttaa tgcggttaca agctcggctt ctgtatatgg cttttgttcc agcttcgaaa
    13801 attgaaatgt aaatatcaaa caaataaatc cgaatgagaa acaaaaaaat atttttttac
    13861 aaaaaaacta tgtgatcctt ttttgtgtaa ctgaataaga gaggcctttt gtaacaatct
    13921 gtatctgtga aaaaagtaaa tatattctgt gagaaatatt ttttcaccgc cacattttcc
    13981 gtggctcaaa actaaccgta tgaaacagga aataagaata atcaggatgc tgatcgttat
    14041 aacctgcact ttttccgcag gtataattat cgcaatgttt gtcgcagcag gttatttgta
    14101 aatctatctg caatttgata tttatgaaag ccacccggct gtcccgggtt tttaattaat
    14161 cagttaaatc aacatttatg agaatcattc agttaatggc atataccacc attgccgtag
    14221 tactcctcgc ttcctgccag aaagagaaaa gccggttgga aaacaatttt aatgaagatg
    14281 aaaattctgt aaccgcaaac aagtcttccg gagaagatga atctatatcc caaagaggta
    14341 acaaccgtgg gtttgtttat actctgtcta atgaaactag cggaaataag gtaattactt
    14401 acaaaagggc cgctaacgga tcgcttacct atattgcttc ttacgcttcc gggggaaatg
    14461 gttcaggcgg agggttaggc aaccagggag ccgttattat ttcgaatgac ggcaatgtac
    14521 tgctcgctgt gaatgcagga agcaatacca tttcttcttt caaaataacc ggcaacggat
    14581 taaatctgaa atccactgtc agcagcggtg gtattactcc ggtcagcatt gcacagcata
    14641 acgacctggt gtatgtgctg aacgccggag gtaatgggaa tatttccggt ttcagacttg
    14701 gagaaaatga taaactcaca ccactttcat cttccaccag gccgttgagt tcttccatgg
    14761 ccggagctgc acaggtgtct tttgtaagaa atggaaaggt gctggtgatt accgagaagg
    14821 ccacgaacaa gatcatcact tacacggtta atgcatcggg ccgtcccgaa gcaatgcaca
    14881 gtatcactgc tgcaagtccc accccttttg gttttgcgcc gggaaggttt gggaatattt
    14941 atgtatccga ggcggttggc ggtgcgccga atgccagtaa tgtatcttct tatcacatca
    15001 gttataacgg tagtatttct ctctcagaag gacccgttgc aacagggcaa agtgctgcct
    15061 gctgggtggt tcttacagag aatggccgtt atatctacac caccaataca gcaagcaata
    15121 atctttcttc ctatactgtt catccattca atggaaatat taatctcttt cagtctgcgg
    15181 cagccacttc aggcatgggg cctatagatg cggcgcttac agacaattcg aaatacctct
    15241 atgtgctgaa tgccggaagc cacagtatca cgggttattc ggtagctggt aacggtacac
    15301 tcacagtact gccgggtact attaccgggc tacctgccag tacaaacgga cttgcagctg
    15361 actaacccgt aaaagaaaat tggttaatgg tgtatataat cccggtgcca ttacggtgcc
    15421 ggggttttaa aaaaacaagt atgatgaaaa atattattat agcttctgca atgcttatgg
    15481 cattaattag ttcctgtaaa aagaatgtgc cggacaatat acaggaacag gtgccccggg
    15541 gtaccgtatt aaaatcaggt acatttgttt cgaacggtaa aactacaagt ggtacagcca
    15601 ggattgtggc aggcgaaaac aatacggtaa agttggtttt tgaaaatttc agtactggta
    15661 atggccctga tgtacgggta tggctttctc caaatactac tgccactact taccttgaac
    15721 tgggagcact gaaagctgtg aatggaacat tctcttatga tctaaacagc agcattgatt
    15781 atactgtcaa taacagggtg cttatctggt gtgaggatgt gaacctgctt tttggccatg
    15841 ctgttttgca gtaatattct gcaagggttt accagtaatt ggtgggaagt attatggtta
    15901 atgctttaca cccggatatt tactggcaaa tagcagttca tagtccgggt tattctttag
    15961 ctgctgaata acatagtgca gcttttcaat gtcttctgtt ttttgaaaag cctggtcatg
    16021 tgccagcagc accaggtgcc ccggtgtttt tgttttacct gctgccagca tattatcaat
    16081 acggcgcagt aataagccgg tggcagtatc caccgccagt gttttgtggt caaacatcca
    16141 ttcaatatcc caacccatta tggcaaagcc cgcctggtga agggaatcaa tggccgtctt
    16201 gctttcatag atatccgtgt gatcgatcgt gtcgatacgc caggcattac gaccgggcat
    16261 acgggcaata ctattggaaa agcctgcaat tttttgtgcc tgtttaaaat catccaccac
    16321 tttacccggt tgttcataaa aagaagtata acggttatgg gcatgggtgt aactgtgatt
    16381 gcagcgttcg atagtagtgt ctgacttcag catctcccag gtttcttttt gccgggggct
    16441 gtcgaatata tgtacgccta cgatgaagaa gctggcggca atattttctt ctttcacggc
    16501 ggccagtaca taacgggtgc ctttattggg accatcatca aaagtgatat agatcttttt
    16561 tttgcgctca gcggctttag caatggagtc agcttttttt tgttgttg
//
LOCUS       DAORTI010000074        16552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3202_length_16552_cov_7.967691, whole genome shotgun sequence.
ACCESSION   DAORTI010000074 DAORTI010000000
VERSION     DAORTI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16552
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_16552_cov_7.967691"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2922)
                     /locus_tag="PLO70_10265"
     CDS             complement(4..2922)
                     /locus_tag="PLO70_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQZ74895.1"
                     /translation="MKDALSQLRKQLRGELFTDNTIRTLYATDASAYREMPLAVAIPK
                     SVDDLKALIAFARQQKTSLIPRTAGTSLAGQVVGNGIVVDVSKHFTAILELNTTESWV
                     RVQPGVIRDELNMFLKPYGLFFGPETSTANRAMIGGMVGNNSCGSNSVVYRSTREHLL
                     EVKALLSDGSEAVFNAISLDDFHAKCEGDTLEGSIYRKVRSILSNYDNQEEIRKEFPK
                     KTVERRNTGYALDLLLDTAPFTAGGEAFNFCKLIAGSEGTLAFITEIKLNIVPLPPKE
                     TGLLCVHFNSIDESLRANLIGLKYKPSASELIDHYILECTKDNIEQRKNRFFVQGDPA
                     AILVIEFCRETREEIIAITQQVESEMRSNGLGYHFPVLFGADSKKIWTLRKAGLGLLS
                     NLPGDAKAVPVIEDTAVDVNDLPAYIRDFNEILKKHNLYSVHYAHAGSGELHLRPIIN
                     LKTIEGNQLFRTIAEEIATLVKKYNGSLSGEHGDGRLRGEFIKQMVGAKNYALLKEIK
                     KAWDPENIFNPDKIVDTPSMNTMLRYIPGHQTPAFKTHFRFNNQDVLQHAEQCNGSGD
                     CRKTSLSGGTMCPSFMATKNEKDTTRARANILREFLTNSNKSNRFDHKEIYEVMDLCL
                     SCKGCKSECPSNVDVAKLKAEFLQHYYDANGVPFRSKLIANFTASAKLGALAPGLYNF
                     AVTNTFTGNIIKKLSGFAVKRSMPILYKTTLQQWLKKYKKENPFNSGKKVFLFCDEFT
                     NYNDTEIGIKAILLLEKLGYEVTIPEHLESSRTWLSKGLVRKAKKIINTNIELLHPVV
                     SVDTPMIGIEPSAILTFRDEYIDLATDENLTKAKELSDAVFCIDEFIANEFAAGKIDA
                     ALFTNEKRHIRLHGHCQQKALSTITLTSSALSIPKNYTVEIIPSGCCGMAGSFGYEKE
                     HYDISMQIGELVLFPAIRTAAPETIIAAPGTSCRHQIKDGTGVKAKHPVEILWEAIEK
                     "
     gene            3008..3811
                     /locus_tag="PLO70_10270"
     CDS             3008..3811
                     /locus_tag="PLO70_10270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ74896.1"
                     /translation="MSRDYSAYFSANKELWNKRTVVHKDSSFYNLKGFMSGENVLTPI
                     ELSELGDVRDKKLLHLQCHFGMDSLNWARLGAEVTGVDFSDQAVKEAIQLTQESGLEA
                     KFICCNVYDLKQHLEEKFDIVFTSYGTIGWLPDLDKWADIISYYLKPGGTFYMADFHP
                     VLWMFDDEFTHIKYSYDNKEIIVVESEGTYTDKNADISGKEYGWNHSISEILNALIKK
                     GLRLHHFNEFMYSPYPCFNNMVETEKNKWHIKGMEDKIPMVYSIRAGKQ"
     gene            3848..4561
                     /gene="pyrH"
                     /locus_tag="PLO70_10275"
     CDS             3848..4561
                     /gene="pyrH"
                     /locus_tag="PLO70_10275"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQZ74897.1"
                     /translation="MLPKYKRILLKLSGEALMGDKSFGFDNDVIAQYAQDIKSITDLG
                     VQVAIVIGGGNIYRGMNEAETGIERAHGDYMGMLATVINGMALQAGLEKIGVYTRLQS
                     AIKMEQIAEPYIRRRAIRHVEKGRVVIFGAGTGNPYFTTDTAGSLRAIEISADVILKG
                     TRVDGIYTADPEKDPTATRYNVITFQECLSQNLKVMDMTAFTLCMENNLPIIVFNMNK
                     PDNLRRVVTGEKVGTLVRG"
     gene            4704..5729
                     /locus_tag="PLO70_10280"
     CDS             4704..5729
                     /locus_tag="PLO70_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74898.1"
                     /translation="MATLSLSVIEIIVLMLGAITLGITIHFFIVSRKSLRETTSDVTG
                     KINKELEMWKLKYFNDTEVRDKELEILKKKLADADENSDIYSIEAEELRIVNKKLQAE
                     IESYRKAAPPLATTSSEKPDYIEQLRMAQTSLLQHNEKINQLLGQIDIVKETEERQQE
                     IIKTNEELSSQIDDLRFKLSQKEKEINNVRQKEHLTTEMNAMLDSAYSEFNVLQDKIQ
                     KLEHQVNVSKRINLEYEELKEVQHKMTRDFEEQKLKYQAAVEENRQLQLAIAEAEDSL
                     KETEFQRHQLQKRVAYLEELNNDMQAMSDANKKLEGQLKRIGELESMLNVIAEERDQL
                     IQRKTNL"
     gene            complement(5947..6066)
                     /locus_tag="PLO70_10285"
     CDS             complement(5947..6066)
                     /locus_tag="PLO70_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HQZ74899.1"
                     /translation="MGRGDKKTAKGKRFKGSFGKSRPVTPTAAKKKAAAKKSK"
     gene            complement(6176..6475)
                     /locus_tag="PLO70_10290"
     CDS             complement(6176..6475)
                     /locus_tag="PLO70_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQZ74900.1"
                     /translation="MLAKHSKIQIMNKAELIAKVADDAGITKTQANAALDSFVEAVTK
                     TLKGGGKVTLVGFGTFSVSKRAARNGRNPQTGAVIKIKARKVAKFKAGKELSSKM"
     gene            complement(6741..7046)
                     /locus_tag="PLO70_10295"
     CDS             complement(6741..7046)
                     /locus_tag="PLO70_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HQZ74901.1"
                     /translation="MIIYNVTVKVNHSIAESWIKWLKEEHIPAIINTGCFSHATILHL
                     IEAGDEEGITYAIQYHTAGKELYTRYIENFADEMRKRAIDKWGDNFIAFRTVMQVVN"
     gene            complement(7055..7822)
                     /locus_tag="PLO70_10300"
     CDS             complement(7055..7822)
                     /locus_tag="PLO70_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQZ74902.1"
                     /translation="MRIISYNVNGIRAAIKKGFIDWLKTNPADVICVQETKAVKGDVD
                     TKLLNDLGYYDYWFSAQKKGYSGVAVFSKIKPDKVELGNGHGPSDDEGRVIQLDFGDT
                     RLINAYFPSGTSGDERQDFKYVWLDECFAWLNKLKKKHPKLILCGDYNIAHKEIDIHD
                     PKGNKNSSGFLPAEREWLTKLLESGWIDTFREFHPEPHRYSWWSQRFPTVRLNNKGWR
                     IDYINVTEPLKKQLKDAEIFPDIKHSDHCPVYLDIKL"
     gene            7924..8670
                     /locus_tag="PLO70_10305"
     CDS             7924..8670
                     /locus_tag="PLO70_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQZ74903.1"
                     /translation="MEAAVKQKKSVLIRPKEHVFLEGPKSRGYELFFALRVFKQFIRG
                     FRALHFTGPCITVFGSARFKEDHPYYIQAREFGKRIAESGMTTITGGGPGIMEAANRG
                     AFEAGGKSVGCNIQLPFEQKANPYLHKSVTFEHFFVRKVLLVKYSYAFIIMPGGFGTM
                     DELFETLTLVQTKTITRFPIVLFGTEYYNPLLEAIQEMEKQDTISPDDMKLVLVTDSI
                     DEAMDHITNYISTHYKVRPRKRHWWLFEKR"
     gene            8722..10863
                     /locus_tag="PLO70_10310"
     CDS             8722..10863
                     /locus_tag="PLO70_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HQZ74904.1"
                     /translation="MLLKRLLISLVLIGLVQLVYADQSINISSPNNTIQLGLLHRTNG
                     DLVYQVLYKGKTTITPSGLGMKLKSPELSLVKFDLIGIDSSIADETWQPVWGEVSSIR
                     NNYKQLVVKLSDRSGSGIQLQIIFRVFDEGIGFRYSFPQQANLNHFIIADELTQFGVS
                     GDHKAFWIPGDFDTNEYPYYTSNLSSVDATGGGAAQEIHAKTYFDKNAVQTPLMMKSV
                     DGIYINIHEAGLINYPAMNLVLDKSSFTFNTHLVPDAVGNKAYLQTPSVTPWRTIIVS
                     DKAAELLASKMILNLNEPSKVANAPSWVKPQKYVGVWWEMHVGKSSWDYSGGQVGSQQ
                     ATRVPHGANTANVKRYIDFAAKNGFSSVLVEGWNTGWEDWFGQWKEDVFDFVTPYPDF
                     DVKELQQYAAARGVKIIMHHETSGSVTNYERWMDTAYRFMKQFGYDAVKTGYVGRIIP
                     RGEHHDGQWMVKHYERVAQKTASYNIMVNMHESVRLTGLHRTYPNWLASEAARGNEFN
                     AWSVGNPPDHETILPFTRLMGGPMDYTPGIFKIKMNYYNPGKTEQVHTTLAKQLALYV
                     TMYSPLQMAADLPENYEAKMDAFQFIKDVAADWDDTKILDAEPGDYIITARKAKGSSN
                     WFVGAITDELARNFSTSLSFLDPGKKYIAAIYRDADNAHWKDNPEAYIIEKFLVDSKT
                     NLKLKLAPGGGTAVSLMPATVEEAKHFKKYK"
     gene            10863..11123
                     /locus_tag="PLO70_10315"
     CDS             10863..11123
                     /locus_tag="PLO70_10315"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HQZ74905.1"
                     /translation="MTAADILGYAAGALTAFTFLPQVLKTWKEKSAKDVALNMFIIAF
                     INEIMWLAYGVMLNNWVIILTNAVMLVMSGIMIWLKMRYNHQ"
     gene            11132..11743
                     /locus_tag="PLO70_10320"
     CDS             11132..11743
                     /locus_tag="PLO70_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQZ74906.1"
                     /translation="MTIKAIIWDLGGVLIDWNPKYVFTETYFESAEKRDYFFTHICTN
                     DWNEEQDAGRSIVEATQELVAAHPGWEKAIRDYYGRWTEMLKSPIYDTVDIFRHLKDS
                     NKYKLYALTNWQANLFDIALVRYDFLHWFDGRVVSGEEKTRKPFPEFYQRLLTRYNIN
                     PAEALFIDDNLRNVKAAEDLGIKSIHFQSPEQLKQEFRNISVF"
     gene            11800..12348
                     /locus_tag="PLO70_10325"
     CDS             11800..12348
                     /locus_tag="PLO70_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQZ74907.1"
                     /translation="MTPLIIIIVLVIILILVLLYNNLIRKKNAVENSFFSIDVMLKKR
                     YDLIPQLVEAVKGYMIHERDLLSSLTALRQKAMENNLPANEKVRLANQVNNILQQIIV
                     NFENYPQLKASENFLRLQGAINEAEEQLAASRRFYNAAVNDYHNAIETFPSSLVASLA
                     GMSKKEYFTIPDSEKGVPALNI"
     gene            12349..>12773
                     /locus_tag="PLO70_10330"
     CDS             12349..>12773
                     /locus_tag="PLO70_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74908.1"
                     /translation="MNSPHMSLEKNFNTFYEKELQGLLIPLETQRKKIKKYGAAGFIL
                     LGTAVILFIISAASHNKVSVVIAFVLLLAAFVLLIIFGNKKKSFAAGFKESVVRKIIR
                     FIDPSLQYSPGLKISKADYLNSGLFVEQPDRYSGDDYIEG"
     assembly_gap    12774..12873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12874..15374)
                     /gene="metH"
                     /locus_tag="PLO70_10335"
     CDS             complement(<12874..15374)
                     /gene="metH"
                     /locus_tag="PLO70_10335"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQZ74909.1"
                     /translation="MQETTFIKPYLRLAGLEPLVIRPETNFVNIGERTNVTGSKKFAR
                     LIKENKYEEALSVARQQVENGAQVLDVNMDDALLDGVLAMTTFLNLVQSEPDIAKIPI
                     MIDSSKFEIIEAGLKCVQGKCIVNSISMKEGEAKFIEQAIICQSYGASVIVMAFDEQG
                     QADTKQRKVEICRRAYKILTEQVGYDPQDIIFDPNIFAVATGLEEHNNYGVDFIEATR
                     EIKQMMPLTKVSGGVSNLSFSFRGNDHVREAMHSVFLYHAIKAGMDMGIVNAGQLVVY
                     DEIEPQLRQLCEDVILNRNNDNNEATEKLISFAETVKAKGKEIIKDDAWRNTTVEERL
                     RHALVNGITDYIDADTEEARLKYPAPLEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKS
                     ARVMKKSVAVLTPFIEAEKEERRQAHLAAGTTNEEGAGAPKVLLATVKGDVHDIGKNI
                     VGVVLGCNGYDIIDLGVMVPADKILDTAEKEKADIIGLSGLITPSLDEMVHVAHEMKR
                     RSMKQPLLIGGATTSRMHTAVKIAPQYDNGVLHILDASRSVTAVSTLLSKEKEILLQK
                     TKDEYEGLRNQFLNKNKKDLIPYKEAVITKEYFDWKNYKPAKPAQDGVKVLKEFDLGT
                     IAKYIDWGPFFIAWEMPGHYPQVLEDKIFGTEAKRLLNDAQKLIEKIVAEKWFTADGV
                     IGFWPAASNNADTIILQTDNGEVKLESLRQQLKKAVGQPSYSLADFICPAELGTDYMG
                     SFAITIHGARKHIDRFAADHDEYNKILVQILADRFVEAFAECLHQKVRKEYWGYEKNE
                     TLTNEQLIKEEYKGIRPAPGYPACPDHTEKLKLFEL"
     gene            complement(15470..16471)
                     /locus_tag="PLO70_10340"
     CDS             complement(15470..16471)
                     /locus_tag="PLO70_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQZ74910.1"
                     /translation="MKTIQDCLKERILIIDGAMGTMIQRHKLTEADYRGDRFKDWHCD
                     VKGNNDLLNITQPQIITGIHKQYLDAGADIIETNTFSSTAIAMADYEMQSLAYELNVA
                     AAKCAREAIKQSGKQAWVAGAIGPLNKTLSLSPDVNNPGFRAVTFDEVVTAYYEQVKG
                     LVDGGVDILLIETIFDTLNAKGAIFAIKKYFRDTKKESLPVMISGTITDASGRTLSGQ
                     TLEAFYTSIMHARPLSVGLNCALGAKEMRPHIEELSQIASCYVSAYPNAGLPNAMGEY
                     DEHPEDTGHYLEEWAKEGFVNIVGGCCGTTPEHIKHIADHVKNLKPRQLPVVEKELA"
BASE COUNT         4772 a         3249 c         3322 g         5109 t
ORIGIN      
        1 gaattatttt tcaatcgctt cccacaaaat ttctaccggg tgtttagctt tcacgcctgt
       61 gccatcctta atctggtgcc tgcaactggt gccgggagca gcaataattg tttcaggagc
      121 agcggttcgg atagcaggaa ataatactaa ttccccgatt tgcatgctta tatcatagtg
      181 ttctttctca taaccaaaag aaccagccat gccacaacaa ccgctgggta ttatttcaac
      241 tgtataattt ttgggtattg atagagctga agaagttaaa gtaattgtac tcaatgcttt
      301 ttgctggcaa tgaccatgta atctgatatg ccgcttttca ttggtaaaca acgctgcatc
      361 tattttgccg gctgcaaatt catttgcaat aaactcatct atgcaaaaaa ctgcatcact
      421 cagttccttt gctttggtta aattttcatc tgtagccaga tcaatatatt catccctgaa
      481 tgttaaaatc gccgagggct cgattccgat cataggagta tcgacagaaa caaccgggtg
      541 cagcaattca atgtttgtat tgatgatttt ttttgcttta cgaacaagac ccttgctcag
      601 ccatgtacgg ctgctttcta aatgttcagg aatagttact tcgtaaccta atttttctaa
      661 taataagatt gctttaatac caatttcagt gtcattgtaa ttagtaaact catcacagaa
      721 gagaaagacc ttcttaccag aattgaatgg gttttccttt ttatactttt tgagccattg
      781 ttgtaaagtt gttttatata atatgggcat gcttcgttta acagcaaatc ccgataattt
      841 ttttattata tttcctgtaa aagtattggt gaccgcaaaa ttatacaggc cgggtgctaa
      901 tgctcccaat ttagcagatg ctgtaaaatt tgcaatcagt ttgcttcgaa agggaactcc
      961 attggcatcg tagtaatgtt gtaaaaattc tgctttaagt ttcgctacat ctacattgct
     1021 ggggcattca cttttgcaac ccttacaact caaacaaagg tccattactt cataaatttc
     1081 tttatgatcg aacctgtttg atttattgct gtttgttaaa aactcccgca agatattggc
     1141 cctggccctt gtagtgtctt tttcattttt agtagccatg aaggatgggc acatagtacc
     1201 accacttaat gatgtctttc tgcaatcgcc gctcccatta cattgctcgg catgttgcag
     1261 tacatcctgg ttgttaaacc ggaaatgagt tttaaaagct ggtgtttgat gcccgggaat
     1321 atatcgcagc atggtattca tgctgggggt gtctactatt ttatcaggat taaagatgtt
     1381 ttcaggatcc caggcttttt ttatctcttt taatagtgca tagtttttcg ctcccaccat
     1441 ttgtttgata aactctcctc tcaatcttcc atcaccatgt tccccactta aagagccatt
     1501 atattttttt acaagggttg caatttcttc agcaatcgtt ctgaataatt gattgccttc
     1561 tatcgttttt aaattgataa taggacgtaa atgcagttca ccactgcctg catgtgcata
     1621 gtgaacggag tatagattgt gttttttcag tatctcatta aaatcacgga tataggctgg
     1681 taaatcattt acatctactg cagtgtcttc tattacaggt accgcttttg catcaccggg
     1741 tagattacta agcaatccca gccctgcttt ccggagagtc catatttttt tactgtcggc
     1801 accaaataat accgggaaat gataccccag gccgttggaa cgcatttcag attctacctg
     1861 ttgcgtgatg gcaatgattt cttcacgggt ttccctgcaa aattcaatta ctaaaattgc
     1921 tgcggggtca ccctgtacaa aaaagcggtt cttcctttgt tcaatattat cttttgtaca
     1981 ctccaaaata taatggtcaa ttaattcact ggcactcggt ttatatttca gtccgataag
     2041 attggctctt aagctttcat caatgctgtt aaaatgtaca catagcagac ctgtttcttt
     2101 tggtggcagg ggtactatgt ttaattttat ttcggttata aatgccaatg ttccttctga
     2161 gccagcaata agtttacaaa aattaaacgc ttctccgcct gcggtaaatg gtgcagtatc
     2221 taataataaa tcaagagcat aacctgtatt tctcctttcc actgtttttt taggaaactc
     2281 ttttcgtatc tcctcctggt tatcataatt gcttaagata cttcttacct ttctgtaaat
     2341 agatccttcc agtgtatcgc cttcgcattt tgcatgaaag tcatccagac ttatggcatt
     2401 aaaaactgct tcgctaccat cactcaacaa cgcttttact tccagtaaat gttcccgtgt
     2461 acttctgtag acaacagagt tgctgccgca ggaattgtta ccgaccattc ctccgatcat
     2521 tgccctgttg gcggtagagg tttcgggccc aaaaaataac ccatatggct ttaagaacat
     2581 attgagttca tcccttatga caccgggctg tacccttacc caactttctg ttgtatttag
     2641 ttctaagatg gcggtaaagt gtttcgatac gtcaactaca atgccattac ccacgacctg
     2701 gcctgcaagc gaagtgccag ctgtacgggg aataagggat gttttttgtt gcctggcaaa
     2761 agcaattagt gcttttagat catctacaga ttttggaata gcaacggcca atggcatttc
     2821 ccggtaagca ctggcatccg ttgcgtataa tgttcttata gtattgtcgg taaaaagttc
     2881 cccccgaagc tgtttgcgta attgacttaa tgcatccttc atacttgccc ttgataagag
     2941 gtaaagatac tttaagaaat attgaaatga atggtttggt aatcctgtaa tctgaataca
     3001 tttgtttatg agccgcgatt actcagccta tttttccgct aacaaggaac tctggaacaa
     3061 acgaactgtt gtacataaag actcatcttt ctataacctc aaaggattca tgtccgggga
     3121 aaatgtatta acccccattg aattgagtga gttgggtgat gtaagggata aaaaactatt
     3181 acacctgcaa tgccattttg gaatggatag tttaaactgg gccaggctgg gggctgaagt
     3241 gacaggagtt gatttttctg atcaagctgt caaagaagct atacagctga ctcaagagtc
     3301 gggcctggaa gcaaaattca tttgttgtaa cgtatatgat ttgaaacagc atctggaaga
     3361 gaagtttgac attgtattta cttcttatgg aacaattggc tggttgccag atttggataa
     3421 atgggctgat ataatttcat actaccttaa accgggaggt acgttttata tggcagattt
     3481 tcatccggtt ttatggatgt tcgatgatga gtttactcat ataaaatatt cttatgataa
     3541 caaggagatc atagtagttg aaagtgaagg gacatatact gacaaaaatg ctgacatttc
     3601 gggtaaagag tatggttgga atcacagtat cagcgaaata ttgaatgcgt tgataaaaaa
     3661 aggattacgg ttacatcatt ttaatgaatt tatgtactct ccgtatccct gtttcaacaa
     3721 tatggtagaa acggagaaaa ataaatggca tattaaaggc atggaggaca agataccaat
     3781 ggtatatagc atcagggccg gtaaacaata ggcaaaagcc ttatcttcgc agcgaaaaat
     3841 ttattccatg ctacctaaat acaaacgtat acttcttaaa ctttccggtg aggcattaat
     3901 gggtgataaa agttttggtt ttgataatga tgttattgcc cagtatgcac aggatattaa
     3961 atctattaca gatttgggtg ttcaggtggc cattgttatt ggtgggggta atatctaccg
     4021 ggggatgaat gaggcagaaa ccggaataga aagagctcat ggcgattata tgggtatgct
     4081 ggcaactgtt atcaacggta tggcattaca ggccggcctt gagaaaatag gggtgtacac
     4141 ccgtttgcaa agtgccatca aaatggaaca aatagccgaa ccttatattc gtaggcgggc
     4201 tatccgccat gtggaaaaag gaagagtagt catttttggt gccggtacgg gtaatcctta
     4261 cttcactact gatactgcag gctctctcag ggctattgaa atcagtgctg atgtaatttt
     4321 aaaaggtacc agggtagatg gaatttacac tgctgatccg gaaaaagatc ccacggctac
     4381 caggtacaat gtaattacct tccaggaatg tctttcacag aacctgaagg taatggacat
     4441 gacagctttt acactctgta tggaaaacaa ccttcctatt attgttttca atatgaacaa
     4501 gccggataac ctgcgcaggg ttgtaacagg tgagaaagta ggcacactcg taagaggtta
     4561 aaggtgtttt tcctcagtgt atttactatt ttttatttcg cagggaatac tatcttgtgc
     4621 cttactccaa agcactacct gtgaaaaact attaaccgct atttgacgga tttttaatag
     4681 tttacaacct tactaaacag cttatggcca ctctttcttt atctgttatt gagataattg
     4741 tgctcatgct gggagctatt accctgggaa ttaccattca ttttttcatc gttagccgca
     4801 aatcgcttag ggaaactact tcagatgtga ccggaaagat caataaggaa ctggagatgt
     4861 ggaagcttaa atatttcaat gacacagagg taagggataa ggaacttgag atattgaaaa
     4921 agaaattggc cgatgccgac gagaacagcg atatctattc cattgaagcg gaagaactcc
     4981 ggatcgtaaa taaaaaatta caggctgaaa tagaatctta tcgtaaagca gcacctcctc
     5041 tggctacaac ttcttccgaa aagcctgatt atatcgaaca actacggatg gcacaaacga
     5101 gcctgcttca gcacaacgag aaaataaacc aattactggg gcaaatagat attgtaaaag
     5161 agactgaaga aaggcaacag gaaataatca aaaccaatga ggagctaagt tcacaaattg
     5221 atgatcttcg ctttaagctt tcacaaaagg aaaaggagat caataatgtc cggcagaaag
     5281 agcatctgac cacggaaatg aatgctatgc tggacagtgc ttatagtgag tttaatgtat
     5341 tgcaggataa aattcagaag ctggaacacc aggtaaatgt ttcaaaaaga ataaaccttg
     5401 aatatgaaga gctgaaagaa gtgcagcata aaatgacccg tgattttgaa gaacaaaagc
     5461 taaaatatca agctgcagta gaagaaaatc gccagttgca actggctatt gctgaagcgg
     5521 aggatagttt aaaagaaacg gaatttcagc ggcatcagtt acagaaaagg gtagcttacc
     5581 tggaagaatt aaataatgat atgcaggcaa tgtcagatgc aaataagaaa cttgaggggc
     5641 aattaaaaag aatcggtgaa ctggaaagta tgctgaatgt gattgccgaa gaaagagacc
     5701 agttaataca acggaaaacc aatttgtaat aattcaaaca ggaataaaag ataaattaat
     5761 tttggcgctc ctctcgggga gtgttttttt agtatacttc tgtcaaatga atggcaagtt
     5821 gccatacagg taaagtattt acagtaaaca aaaatggcag actgtttacg ccccaatatg
     5881 cagccggaaa attcattaac aagctgaaaa gattctttca acctcatcaa cttgttaacc
     5941 agttaattat ttactttttt tagcagctgc tttcttttta gctgcagttg gtgtaactgg
     6001 acggctctta ccgaatgaac ctttgaaacg ttttccttta gcagtttttt tatctcctct
     6061 tcccatgata atttatttta gttattatat tttagtacaa aaataaagct ccggcactga
     6121 aataagcaca ggagcttttt taataaggta aatggtacat ccaatagcaa tcggactaca
     6181 ttttgctgga taattctttt ccagctttga atttagcaac ttttcttgct ttgattttaa
     6241 ttacagcacc tgtttgcggg ttgcggccgt tacgggctgc ccttttagat actgagaaag
     6301 taccaaaccc aaccagagta accttgccac cgccttttaa tgtttttgta acagcttcta
     6361 caaaagaatc aagggcagca tttgcctgag tcttggtgat acctgcatca tcggccacct
     6421 ttgcgatcaa ttcagctttg ttcatgattt gtatttttga atgttttgct aacagcgaca
     6481 aatttagacg ctttttgata ccagcaaaaa attttcttct cttttgcaat attacttatc
     6541 ggcccgaagc cgcattggac aaggtttttg gagatttatt gtttttttat tttcatggtc
     6601 aatgttcatc tcaactgctg aaacccttta ctatagcgga tttcgaagtg tgtttgctga
     6661 agtacaggca ggacaagggt ttgagaggaa tatacaattg tttgctattc tgcaaaacaa
     6721 gtaaatattg ttttgcacac tcaattcaca acctgcatta ctgtgcgaaa tgcaataaaa
     6781 ttatctcccc atttatcgat ggctcttttt ctcatttcat cagcaaaatt ttcaatatac
     6841 cgggtataca gctctttgcc ggctgtatgg tattgtatag cataggttat gccttcttca
     6901 tcacccgctt caatcaggtg tagaatagta gcatgcgaaa aacaaccggt attaattatt
     6961 gcgggaatgt gctcttcttt tagccatttt atccagcttt cagcaatgga gtgattaact
     7021 tttactgtga cattataaat tatcatctgc aacgttaaag tttaatatca aggtataccg
     7081 ggcaatgatc agaatgcttt atatcaggaa atatttctgc atctttcagt tgctttttta
     7141 aaggctcagt aacatttata tagtcaatcc tccagccctt attattcaat cgtacggtag
     7201 gaaaacgctg gctccaccag ctgtaccggt ggggttcagg atgaaattca cgaaaagtgt
     7261 ctatccagcc actttccagc aattttgtca gccattctct ttccgccggc aaaaatccgg
     7321 atgaattttt atttcctttc gggtcgtgta tatcgatttc cttatgggca atattatagt
     7381 caccgcaaag aataagttta ggatgttttt ttttcaattt attcagccag gcaaaacatt
     7441 catcaagcca tacatatttg aagtcctgtc tctcatcgcc actggtgccg ctggggaaat
     7501 atgcgttaat aagcctggta tctccaaaat ctaattgaat caccctgcct tcatcatcac
     7561 ttgggccgtg tccattaccg agttcaactt tgtcaggttt tatcttactg aatactgcca
     7621 caccgctgta cccttttttt tgggcactga accaataatc ataatagccc aaatcattca
     7681 gcaattttgt atctacgtcg cccttaactg cctttgtttc ctgcacacat atcacatcgg
     7741 cagggttggt ttttaaccag tcaataaatc cttttttaat cgcagctctt atgccattaa
     7801 cattatagga tatgatacgc atagtatgtt tgtgttttat tgataaaagc gggtaatttt
     7861 aaatattata aaccgatagt aatccggaca ataatcaaaa acaatagcaa tttacatttt
     7921 actatggaag cagcagttaa acaaaaaaaa agtgtgctta ttaggccaaa ggaacatgtt
     7981 tttttggaag gtcctaaaag ccgtggttat gaactcttct ttgcattacg ggtttttaaa
     8041 cagtttattc gtggttttag ggcattgcat ttcaccggac catgtatcac agtatttggt
     8101 tcagccaggt ttaaagagga ccacccttat tatatacaag ccagggaatt tggtaaacga
     8161 attgcagaat ctggcatgac aactattact ggaggcggac cgggtataat ggaagcagcc
     8221 aacagaggag cttttgaggc tggaggtaaa tctgttggat gtaatattca attgcctttt
     8281 gaacagaagg caaatcctta cctgcacaag tcagttactt ttgaacattt ttttgtccgg
     8341 aaggtattgc tggtaaaata ttcttacgct tttattatca tgcctggtgg tttcggtaca
     8401 atggatgagt tattcgaaac gctgaccctg gtgcagacaa agaccataac ccgttttccg
     8461 attgttttat tcggaacgga atattataac cctttgctgg aggcaataca agaaatggaa
     8521 aagcaggata ctatttcacc tgatgacatg aaactggtgc tggtaacaga cagtattgac
     8581 gaagcaatgg atcatatcac caactatatc agcacccatt ataaggtgag gccacgtaaa
     8641 cgccactggt ggctgtttga aaaacgatag ccaaatgtgt actttttaca caaaagtatt
     8701 accttcatgt aaatttttgc catgctttta aaacgattgc tgatcagcct tgtattgatc
     8761 gggctagttc agttggttta tgccgaccag tcaataaata tcagttctcc caataatact
     8821 attcagcttg gtttgctcca tagaaccaac ggagatctgg tatatcaggt cttatataaa
     8881 gggaaaacga caataacccc ctcaggattg ggaatgaaac ttaagagtcc tgaattatca
     8941 cttgtcaaat ttgacctgat aggtattgat tcatctattg cagatgaaac atggcaaccg
     9001 gtttggggtg aagtaagttc gatccgtaac aactacaagc aacttgttgt aaaactttcc
     9061 gaccgttcag gttcaggaat tcaactgcag atcattttca gggtatttga tgaaggaata
     9121 ggatttcgtt attcgtttcc ccaacaggcc aacctcaatc attttattat tgccgatgaa
     9181 ttaacacaat ttggagtaag tggagatcac aaggcatttt ggattcccgg tgattttgat
     9241 accaatgaat acccttatta cacatcgaat ctgagtagtg ttgacgcaac cgggggtggt
     9301 gcagcacagg aaatacatgc taaaacctat tttgataaga atgccgtgca gacaccctta
     9361 atgatgaaat ctgttgatgg aatttatatc aatattcatg aggcaggatt aataaattac
     9421 ccggccatga acctggtact tgataagtca tcttttactt tcaatacaca cctggttccc
     9481 gacgcagttg gaaacaaagc ctatttacaa acaccatcag taactccctg gagaacgatc
     9541 attgttagcg ataaagcggc agaactattg gcctcaaaaa tgattttaaa tttaaatgag
     9601 ccttcaaaag ttgcgaatgc accatcctgg gttaaaccac aaaagtatgt aggtgtatgg
     9661 tgggagatgc atgtgggtaa aagtagctgg gattatagtg gcgggcaggt tggttcgcaa
     9721 caagcgacca gagttcctca tggagctaat actgctaatg tgaaacgcta tatagatttt
     9781 gctgccaaaa atggatttag cagtgtactg gtagaaggct ggaataccgg ctgggaagac
     9841 tggtttggtc agtggaaaga agatgttttt gattttgtta cgccgtatcc cgattttgat
     9901 gtaaaagaat tacagcaata tgcggctgcc agaggcgtga aaattatcat gcatcatgaa
     9961 acttcaggct ctgttaccaa ctatgaaaga tggatggata cggcttaccg ttttatgaag
    10021 caatttggat atgatgcagt gaaaacaggc tatgtgggca gaattattcc ccgtggcgaa
    10081 catcatgacg ggcagtggat ggtaaagcat tatgaaagag tagcgcagaa aacagcctct
    10141 tacaatatca tggttaatat gcatgaatct gttcggctta ccggtttgca tcgtacttac
    10201 ccgaactggt tagccagtga agcagccagg ggaaatgaat ttaatgcatg gagtgtgggc
    10261 aatccacctg accatgaaac aatccttcct tttaccaggt taatgggagg cccgatggac
    10321 tatacacccg ggatatttaa aattaaaatg aactattaca atccgggtaa aacagaacag
    10381 gtacatacta cgctggccaa gcaattagcc ttatacgtta caatgtatag ccctttgcaa
    10441 atggctgccg atcttccgga gaactatgag gcaaaaatgg atgcattcca gttcataaag
    10501 gatgtggcgg cagattggga tgataccaaa atcctggatg cggaacctgg ggactatatc
    10561 attacagccc ggaaagctaa gggcagcagt aactggtttg taggtgctat cacggatgaa
    10621 ttggcgagaa atttttccac ttcactctca ttccttgatc ccggcaaaaa atatattgct
    10681 gcaatttacc gggatgcaga taatgcccac tggaaggata atcccgaagc atatataatt
    10741 gagaaatttc tggtggatag taaaacaaat ttaaaactga aactggcacc gggtggagga
    10801 actgctgtaa gtttaatgcc agctactgta gaagaggcca aacattttaa aaaatataaa
    10861 taatgacagc agcggatata ttgggttacg cagccggtgc attgacagca ttcacttttt
    10921 tgccacaggt attgaaaaca tggaaggaaa aatctgccaa agatgttgcc cttaatatgt
    10981 tcatcattgc atttattaat gaaataatgt ggttggcata tggcgttatg ctaaataact
    11041 gggtcattat cctcactaat gctgttatgc tggtgatgtc cggaattatg atctggctta
    11101 aaatgcgtta taatcatcag tgatcagtac tatgacgatt aaagcaatta tctgggacct
    11161 tggaggcgta ttaattgact ggaaccctaa atatgtattc acagaaactt attttgaatc
    11221 agcggaaaaa agggattatt tttttacgca tatctgtaca aacgactgga atgaggagca
    11281 ggatgccggt cgttctattg tagaagcgac acaggagttg gtggctgccc acccgggatg
    11341 ggaaaaagcc atccgggatt attatgggcg ctggacagaa atgctgaaaa gcccgattta
    11401 cgatacggtt gacatattcc ggcatttaaa agattcaaac aagtataaac tctatgcatt
    11461 gaccaactgg caggcgaacc tttttgatat cgcccttgta cggtatgact tcttacattg
    11521 gtttgatggc agggtggtga gtggtgaaga aaaaacaaga aaacccttcc cggaatttta
    11581 tcagcgattg ctgacgagat ataatattaa ccccgcggaa gcattattta ttgatgataa
    11641 ccttcgaaat gtaaaggccg cagaagattt ggggataaaa tctattcatt tccaaagccc
    11701 ggagcaatta aagcaggagt ttagaaatat cagtgtattt tagtttggaa ttcagcgtta
    11761 cgcagaagaa attaaacgat cacactaact ggtttttgta tgacacccct gatcattatc
    11821 attgtactgg taatcatcct catcctggtt ttgctctata ataaccttat caggaaaaag
    11881 aacgctgttg agaactcatt tttttccata gatgttatgt taaagaagcg gtatgacctg
    11941 ataccacaac tggtagaagc cgtaaagggc tatatgattc acgaaagaga cttacttagc
    12001 agcctcacag cattacggca aaaggcaatg gaaaataact taccagcaaa tgaaaaagta
    12061 agactggcta accaggttaa caatatcctg cagcaaataa tagtgaattt tgagaactac
    12121 ccgcaactga aagccagtga aaattttctg cgattacagg gggccatcaa tgaggctgaa
    12181 gaacagttgg cggcctcaag gcgcttttat aatgcagcag ttaatgatta tcataatgcc
    12241 atcgaaacat ttccttcatc tcttgtcgct tcactggctg ggatgagtaa aaaagaatat
    12301 tttactattc cggattcaga aaaaggagtt cctgcattaa atatataatt gaacagccct
    12361 catatgtccc tggagaaaaa tttcaataca ttttatgaaa aggagctgca gggcctttta
    12421 attccgcttg aaacacaacg gaaaaaaatc aaaaaatatg gagctgccgg ctttatctta
    12481 ctcggtacag ctgttatcct gtttatcata tcagccgcat ctcataataa agtttctgtt
    12541 gtgattgctt ttgtgctgct gctggctgct tttgtgctgc tgattatttt tggtaataaa
    12601 aagaaatcgt ttgctgccgg gtttaaagaa tctgttgtca gaaagatcat ccggtttatt
    12661 gacccctcat tgcaatatag cccgggactg aaaatcagta aagctgatta tttaaacagc
    12721 ggtctttttg tggagcaacc cgatcgctat tcgggcgatg attatattga aggnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagctcaa ataattttaa tttctccgta
    12901 tgatcagggc atgcaggata gcccggggcc ggccggatgc ctttatattc ctcttttatc
    12961 aattgttcat tggtaagggt ttcattcttt tcataacccc aatattcttt tctgactttt
    13021 tggtgcaggc attcagcaaa ggcttctaca aaacgatcgg ccaatatctg aaccaggatt
    13081 ttattatact catcatgatc agccgcaaac cggtctatat gtttcctggc gccatgaatg
    13141 gttattgcaa atgaacccat ataatcggta cccagttctg cagggcagat aaaatctgct
    13201 aatgaataac tgggttgtcc cactgctttc tttaactgtt gacgaagcga ttctaatttt
    13261 acttcaccgt tatctgtctg gagtattatg gtatctgcat tgttgctggc agcgggccag
    13321 aagccgataa ctccatcagc cgtaaaccat ttttcagcta ctattttttc aattagtttt
    13381 tgtgcatcat ttagtagtcg ttttgcttct gtaccgaaga tcttatcctc cagcacctgc
    13441 ggataatgac caggcatttc ccaggcaata aaaaacggtc cccagtcaat atattttgca
    13501 atagtgccga gatcaaattc tttcaacact tttaccccgt cctgagccgg tttggcaggc
    13561 ttataatttt tccagtcaaa atattctttg gtaataaccg cttctttgta ggggatgagg
    13621 tcttttttat tcttattcag gaactggttt cttaatcctt catattcatc ttttgtcttt
    13681 tgaaggagta tttctttctc tttacttaat aaagtactta cagctgttac acttctggaa
    13741 gcatctaaaa tatgtaatac cccattatca tattgcgggg caatttttac agctgtatgc
    13801 atcctggaag ttgtggcgcc accgatcagc aagggctgtt tcatactgcg gcgcttcatt
    13861 tcatgggcca catgtaccat ttcgtctaat gatggagtga tgagaccact taacccaatg
    13921 atatcagctt tttctttttc agcagtatcc aggattttat cggcaggcac cattacgccc
    13981 aggtcaatga tatcatatcc gttacaaccc aagacaacac ccacaatatt tttcccgata
    14041 tcatgaacgt ccccttttac tgtggctaat aaaacctttg gagctccggc tccctcttca
    14101 tttgttgtgc cggctgcaag atgtgcctgg cgtcgctctt ctttttctgc ttcaataaaa
    14161 ggagtgagga cagctactga tttcttcatc actcttgcac tttttactac ttgtggcagg
    14221 aacatttttc cggctccgaa taagtcaccc actacattca tgccatccat cagcgggcct
    14281 tcaattactt ccaacggagc aggatatttt aatctggctt cttccgtgtc tgcatctatg
    14341 taatcagtaa tgccattgac cagagcatgc ctcagtcttt cttcaacggt tgtatttctc
    14401 catgcatcat cctttattat ttctttacct tttgctttta cggtctcggc aaaagagatc
    14461 agcttttcag ttgcttcgtt attatcatta ttccggttca gaattacatc ttcacaaagt
    14521 tgccttagtt ggggttcaat ttcatcatat accaccagct gacctgcatt tacaatcccc
    14581 atatccatgc cagccttaat ggcatgatat aagaatacac tgtgcatcgc ttctcttaca
    14641 tggtcattac cccggaaaga gaaagaaaga ttactgacac caccactaac tttggtcaat
    14701 ggcatcattt gttttatttc acgggttgct tcaataaaat caaccccata attattatgc
    14761 tcttccaggc ctgttgcaac agcaaatatg tttgggtcga aaataatatc ctggggatca
    14821 taacctactt gttcggttag tattttgtaa gccctccggc atatctcaac ttttcgttgt
    14881 ttggtatcgg cctgcccctg ttcatcaaaa gccattacaa taacggaggc gccgtaactt
    14941 tgacagatta tagcctgttc tatgaatttg gcttcgcctt ctttcattga gatggagttg
    15001 actatacatt ttccctgtac gcattttaaa ccggcttcaa tgatctcaaa cttggagctg
    15061 tcgatcatga tgggaatttt tgcaatatcc ggttcactct gaacgaggtt gagaaaagtg
    15121 gtcatggcta atacaccgtc cagtaaagcg tcgtccatat ttacatccag cacctgggcg
    15181 ccattctcaa cctgttgcct ggccacgctg agcgcctctt catatttatt ttctttgata
    15241 agacgggcaa attttttcga gccggtaaca ttggttcttt caccaatatt tacaaaattg
    15301 gtttctggcc ttataactaa tggttctaat cctgctaatc tcaggtaagg tttgataaag
    15361 gtggtctctt gcatctatga attcttttta agaaacaatt ttttgattat tgaaaaattg
    15421 tttacaataa gtatgaggat ggatattact aataacagtt gtgcggttgt catgctaatt
    15481 ctttttcaac tacgggcagt tgtctgggtt taagattttt tacatggtca gctatatgct
    15541 taatatgctc cggtgtagtc ccacagcaac caccaacaat gttcacaaat ccttctttgg
    15601 cccattcttc caggtaatgg cccgtatctt caggatgttc atcgtattct cccattgcat
    15661 tgggtaatcc tgcattaggg tatgcggaaa catagcaact ggcaatttgc gacagttctt
    15721 caatatgtgg ccgcatttct tttgcaccta atgcacagtt cagcccaaca ctcagcggcc
    15781 tggcatgcat gattgaagta tagaaagctt cgagggtctg accacttaat gttctgcctg
    15841 aggcatcggt gatagtgcca ctgatcatta ccggcaatga ttccttttta gtatccctga
    15901 aatatttctt gatagcaaaa atggcccctt tggcatttaa tgtgtcaaag atggtttcga
    15961 ttaagagaat atcaacacca ccgtctacta atccttttac ctgctcgtaa taagcagtta
    16021 ctacctcatc aaatgtaacc gccctgaaac cgggattatt tacatccggg gaaagcgaca
    16081 atgttttgtt taatggcccg atagctcctg caacccatgc ctgtttaccg gattgtttaa
    16141 tggcttcccg ggcacatttt gctgcggcta cgtttaattc ataagcgagg gactgcattt
    16201 cgtaatccgc catggcaata gcagtactac tgaatgtatt ggtttcaata atatctgcac
    16261 cggcatcgag gtattgtttg tggatacctg ttatgatttg aggctgagta atattcagca
    16321 ggtcattatt gccttttaca tcgcaatgcc agtctttgaa cctgtcacca cgataatcag
    16381 cttcagtgag tttatgccgc tgaatcatgg tacccatggc gccatcaatg atcaaaatgc
    16441 gttccttcaa acaatcctgg attgttttca taaacttatg ttttgcgaac tataaacgtt
    16501 gggaaaccag gagggaattc cgtaccgttt attagttctg ttttttagtg ca
//
LOCUS       DAORTI010000075        16504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3221_length_16504_cov_6.704177, whole genome shotgun sequence.
ACCESSION   DAORTI010000075 DAORTI010000000
VERSION     DAORTI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_16504_cov_6.704177"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLO70_10345"
     CDS             <1..776
                     /locus_tag="PLO70_10345"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HQZ74911.1"
                     /translation="ENDTVLNDLVRHKLIEKRNYKIVHYDSRDFRGIDVGLLYNPKYF
                     IVEASDKLFVQLPGGSKDAYFTRDILWVKGKLGGETIHIYVNHWPSRSGGEQRSAPAR
                     NAAAQVCKNHMDSIAKLEPNAKVIVMGDLNDDPTNESVEGILKAKGKERDVRAGGMFN
                     PWMDLYKKGIGTLAYQDAWGLFDQILISHAWLNKDQTGFFFYQQHIFNKEYLVENKGR
                     YKGYPMRTWDGNTYRGGYSDHFPTYLIMLREVKKEKKGF"
     gene            975..1331
                     /gene="rpsF"
                     /locus_tag="PLO70_10350"
     CDS             975..1331
                     /gene="rpsF"
                     /locus_tag="PLO70_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQZ74912.1"
                     /translation="MNNYELMVIFTPVLSDDEFKAEQKKYAKLVTDAGGSIVAENPWG
                     LKSLAYPIQKKTTGLYWVMEYTAQSDFNEKLKIQLLRDESVLRHLCTKLDKYAVEYNA
                     RKKSGVKSGTEKTVEA"
     gene            1336..1599
                     /gene="rpsR"
                     /locus_tag="PLO70_10355"
     CDS             1336..1599
                     /gene="rpsR"
                     /locus_tag="PLO70_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQZ74913.1"
                     /translation="MAKNEIKYLTAIKSDKREKKFCRFKKYGIRYIDYKDVEFLKKFL
                     NEQGKLLPRRLSGNSLKFQRKVSDAVKKARQMALLPYVTDLLK"
     gene            1678..2124
                     /gene="rplI"
                     /locus_tag="PLO70_10360"
     CDS             1678..2124
                     /gene="rplI"
                     /locus_tag="PLO70_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQZ74914.1"
                     /translation="MEIILIQDVDNLGAKNEVVKVKNGYARNYLLPRQLAVENSPSNK
                     KQLEERLKQVRKKEDKMLAEINSVIAKLNEAPLKLGAKTGTSGKIFGSITSLQLSRAI
                     REQKGYEIDRKRISIPEDVKEIGTYKATIDFGNGKSTEVEFEVIAE"
     gene            2393..2797
                     /locus_tag="PLO70_10365"
     CDS             2393..2797
                     /locus_tag="PLO70_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQZ74915.1"
                     /translation="MNIYVGNLSWNLKDQDLSNLFATHGEVASAKIVTDKFTNRSKGF
                     GFVEMPNDDQAQAAIAALNGTEVDGRNIVVNESRPKQEGGGGGGFKKRSFGSGGGGYK
                     GGSGGGGGYKGKSGGGGGGGYNRDRGGYNNDY"
     gene            complement(2865..>3408)
                     /locus_tag="PLO70_10370"
     CDS             complement(2865..>3408)
                     /locus_tag="PLO70_10370"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQZ74916.1"
                     /translation="TSVKVEADENLLEYVIVENDGGTLEIHQKQGINLKPSRSIKVYV
                     SGNAFRHFKASGACDIFGENKITSDEKISIDLSGASDIKMELKAPGVDAELSGAGTIT
                     LRGETKDFTVDGSGSTDIKCYELLAENVNVKLSGAGDAQVFASVKLDVKVSGAADIKY
                     KGNPVISQQVSGAGGVKKAE"
     assembly_gap    3409..3508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3509..5092
                     /gene="guaA"
                     /locus_tag="PLO70_10375"
     CDS             <3509..5092
                     /gene="guaA"
                     /locus_tag="PLO70_10375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQZ74917.1"
                     /translation="HSRTSHSFLYLRSTMTEKILILDFGSQYTQLIARAVREANVYCE
                     IIPYYKPFEFEPGLKGIILSGSPFSVNEEKAPDVDVQNFITKVPVLGVCYGAQLTAKR
                     FGGIVAKSNKREYGRAILQCKKEDVLLKDVSPQSQVWMSHSDTIKQLPTGFELLADTE
                     SIPVAAFKKSVKGTPDFPLYGVQFHPEVYHSKEGKKILGNFLVNICGCSQDWTPAHFI
                     TDTVEALKKQIGDRKVIMALSGGVDSTVAATLIHRAIGDRLFGIFVDNGVLRKNEFES
                     VLKTYAQLGLNVKGINAREHFYTKLAGKTDPEEKRKIIGSMFIDVFQEEAKKIEGVGL
                     LGQGTIYPDVIESVSVHGPSVTIKSHHNVGGLPDHLHLELVEPLRSLFKDEVRKVGRE
                     LGIPAEMIDRHPFPGPGLAIRILGEITEEKVQLLQDADHLYIQALKDNNLYATVWQAG
                     AILLPVKSVGVMGDERTYEFTVALRAVTSVDGMTADWAHLPYEFLANISNEIINNVRG
                     INRVVYDISSKPPATIEWE"
     gene            5116..6291
                     /locus_tag="PLO70_10380"
     CDS             5116..6291
                     /locus_tag="PLO70_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HQZ74918.1"
                     /translation="MRIYLLSVFACIAFFQNIHAQGDTTKYKIAIFAPLYLDSAFDAA
                     NEYRYAKNVFPKFINPGLEFYEGAQLALDSLNKEQAPLEVHIFDTRSGKETIAEQLAK
                     PELKNIELIIAYCSAAEVKIFADAGLQKNIPVINVNLPNDGGVTANPFFVLLNSTLKT
                     QCEGIYRHIQKYYSLNPLIVFRKKGQLEDRIKTYFDDFGKSTLSTPLKLKYIDLTDSF
                     TVNQLRPYLDTINPTLCIAGSLDENFGKRLTQQLASLTKLKYKAAVMGMPTWDAIKDF
                     NKPDYKGIEIIYSTPFYNSRTDKVSQSIIEHFNTKMYARPSDMVMRGYETVWRFAKLL
                     IQYKSDFASNLTRKDYSVFREIDIQPVLNKQSMTLDYFENKKLYFLKWMDGVIKAVN"
     gene            6357..8822
                     /locus_tag="PLO70_10385"
     CDS             6357..8822
                     /locus_tag="PLO70_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HQZ74919.1"
                     /translation="MQLTKGYTTITDWLEGKGFKPFLFQEQTWQHIITGESGLVNAPT
                     GCGKTFSVFLGSIIDFINRHPADYKKKAGSGLQLLWITPLRALAKDIGRAMQEVIEEL
                     DMSWEIGIRNGDTSTAERTKQKKRMPEVLIITPESLHLLLAQKGYPDIFKSLKIIAVD
                     EWHELLGSKRGVQVELAISRIIGCGNSGVSIWGISATIGNLEEAKEVLLSTLKQNGVI
                     VKATIQKQIEVESIIPDEIEKYPWAGHLGIKLAHKIIPIIQQSRTTLIFINTRGMSET
                     WYQTLLDVCPDLAGSIALHHGSIDMELRTWIEDNLHTGNLKAVVCTASLDLGVDFRPV
                     ETVIQVGSPKGVARFLQRAGRSGHSPDAISKIYFLPTHSLELMEAAALKNAMTTGIIE
                     SRQPMLLCYDVLLQFLNTLAISDGFKPEILYDEITATYCYRELLKEEWDNILHFLTEG
                     GLALHQYDDYKKIEAEDGLYKIKSRKVAMRHRLHIGTIVSDAMMKVKFMTGGYVGVIE
                     EYFISRLEPGDVFTLAGRNLEFVLIKEMTALVRKSAKKKSIIPSWTGGRMPLSANLGK
                     KLRETLDQSGDKNRMPGVRELEVLSPLFELQKNLSHVPKQNELLIEKIETKDGFHVFV
                     YPFEGRLVHEAMAAILAYRISRIRPITFSFAMNDYGFELLSDQTIPLDDKNVYALFSP
                     QNLLNDIQRSVNSTEMARRKFRDVAVIGGLIFQGMPGEKKKARHLQSSASLLFNVFSE
                     YDPANILLRQAYQEVLDQQMEEARLRDMLQRIQQSNIIITHPQQLTPFCFPIKVDSIR
                     EDLSSEKLEDRVKKMQQQLEKGE"
     gene            8854..9522
                     /gene="pdeM"
                     /locus_tag="PLO70_10390"
     CDS             8854..9522
                     /gene="pdeM"
                     /locus_tag="PLO70_10390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HQZ74920.1"
                     /translation="MQNPVPHIIRNNTFWVSPERCLLWEEENTLIVADLHLGKSGHFR
                     KSGIGIPQSIYKADLQRLMAQLYLYKVDRLIIAGDLTHSTANKELDLFLKWRKDFSLL
                     HIDLVKGNHDILADSWYEEAAIKVSTWKMKTGPFIFLHDLKAEKDLTAEEKGLYRFTG
                     HLHPAINLKGKGKQSLKFPCFYFTKEYCILPAFSRFTAGFKVNPEKDETVFAIVEDGI
                     MKLP"
     gene            complement(9519..11972)
                     /locus_tag="PLO70_10395"
     CDS             complement(9519..11972)
                     /locus_tag="PLO70_10395"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQZ74921.1"
                     /translation="MRLKNYICIPFLLLCFQSFSQVKTTVAVKVTQAPKIDGVLDDGV
                     WANAPVLGDFIQNSPTFGLPCSQKTEVRIVYDNTAIYVGAYLYDDPALIRKQFTARDG
                     EQQTDVDYFSIFFDTYNDKQNGFQFLVTTANVQTDARLTPSFTGDFGSYGDKTWDAVW
                     ESKVSMQPDGWTVEMRIPYISLRFAKKDVQDWGLQLLRFMRRNNESNFWSPVNPKVNG
                     FVNQFGAFTNLQNIVPPLRLSFSPYVTTGYRTTPVGNTYLNEWLRNGGMDVKYGINES
                     FTLDATLIPDFGQVISDNVVNNLTPYEVQFQENRQFFTEGTEIFNKAKLFYSRRVGAM
                     PGGYYDVLGIADADPNIEIVKNPSRTQLYNAIKFSGRTKKKLGIGIFNAVTAPIHAII
                     RDRTTNEETKIETESLANYSIIVLDQAFKGRSSLTFTNTNVMRNGDARDANVTALDLA
                     LYDKKNMYSFQGTARYSKIWGTDPYDGYNTTVKYGKVSGNWQYYVQGNVESKNYDPND
                     LGILLAANEISYRGNLSYRKFVPTKNFINYSYSIAPRLQYLYKPYAYSKFDVTATAFW
                     IFRNFWDVTLTSNVIPGWERNYFELRTDGRPLSYPANYIFQVEGSTDSRKRVFVRFGS
                     TYALAPKYNNVYTGISLGMRYRFSDKFSLDLQTDSHFEENQLGYAFRREVNGEPIVGF
                     RDNRDFTSVLSGIYNFAYRLNLTLRARHYWNRVNYISFHNVDDKGYLLSRLFIPGQDE
                     NFNVFNVDAFLTWDFRLGSRLILGYKNWLGEEEMVNIGTGRNTYLKNLGNTFDLRHGN
                     EFTVRFIYFLDYNQLRKKR"
     gene            complement(12071..12820)
                     /locus_tag="PLO70_10400"
     CDS             complement(12071..12820)
                     /locus_tag="PLO70_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQZ74922.1"
                     /translation="MAISNQNLKYLRKLRGWTQEEFANKLRIKRSLLGAYEEERAEPR
                     IDVLEVVCDIFKLTLDDILRKDLSDNKSNYLAKRRALKLASGRPDIPFVPVKAAAGYL
                     AGYADPEFIDELNTFTLPMLTGGNYRAFEIIGDSMLPTPSGSVIVGEKVDNLDEVKNN
                     AACIVISRNEGIVYKRVQKNGRLKNKLTLVSDNPSFHPYTVNAEEVLEMWQAQVVISR
                     ANTQNRWDMAQLANVVSDLQQQVTSLKKRMN"
     gene            12927..13763
                     /gene="surE"
                     /locus_tag="PLO70_10405"
     CDS             12927..13763
                     /gene="surE"
                     /locus_tag="PLO70_10405"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQZ74923.1"
                     /translation="MKKKASPTPKKNSPAKKKVAKPSVKEKPIILITNDDGITAPGIR
                     NLVEAVKDIGKIVVVAPDKPQSGMGHAITIGQPLRLHKLEVFDGVESYACTGTPVDCV
                     KLAVDKVLHRKPDLCLSGINHGANHSINVIYSGTMSAAVEAAIESIPSAGFSLLDYSI
                     EADFTGAKKFAKLIVEKMLTTKLDKHTVFNVNVPSIHVDLLKGIKICRQAYAKYEEDF
                     IERNDPHGRHYYWLTGEFVNFDKGKDTDVWALANNYVSVVPVQFDLTNYVLKAKLEKS
                     WK"
     gene            13763..14059
                     /locus_tag="PLO70_10410"
     CDS             13763..14059
                     /locus_tag="PLO70_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74924.1"
                     /translation="MILTKDNLKLGLVLGLTGPIIGLVAIYFLKFSSFTFIEFLDNFI
                     HENRLITSIGSLSLLANAVLFTIYVNTHRDNTAKGIFIVTLIYGIGILVLKIFN"
     gene            14079..15191
                     /gene="lpxB"
                     /locus_tag="PLO70_10415"
     CDS             14079..15191
                     /gene="lpxB"
                     /locus_tag="PLO70_10415"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQZ74925.1"
                     /translation="MKYYIIAGEASGDLHGSNLIKELKKHDSAAQIRCWGGDKMQEQG
                     GELVKHYRDLAFMGFAEVLLNLRTIFRNLAFCKEDIQQYQPDAIILIDYPGFNLRIAK
                     WAKKQKHRVIFYISPQVWAWKENRVKMMKECIDKMLVILPFEKDYFKNKWNWEVEYVG
                     HPLVEVIETRLSESGNEKFSDNPIVALLPGSRKQEILKKLPVMLEVSKSFPAYQFIVA
                     KAPGTDEAFYNQLVKDYSNVSYVTNKTYDLLLQSKAALVTSGTATLETALFNVPEVVC
                     YKGSWLSYQIGKRLVKVKYISLVNLIMDKLVVKELIQADLTVEKLKTELSELLNNENR
                     IREVKTDYSLLKQMLHKDGDASAKAAASILQFLSGK"
     gene            complement(15180..15341)
                     /locus_tag="PLO70_10420"
     CDS             complement(15180..15341)
                     /locus_tag="PLO70_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74926.1"
                     /translation="MGVWNQLAEYLFLKKKDPNRPKDKWIGYMHGINRISIFVFILCL
                     IILAVKLLT"
     gene            15461..16399
                     /locus_tag="PLO70_10425"
     CDS             15461..16399
                     /locus_tag="PLO70_10425"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HQZ74927.1"
                     /translation="MHFSFITYSFTPVPQNQAMAYIKLLRPKDWAKNFFLFIPLFFAG
                     ELFNMEKIIAILWGFLSFSFIASSIYIINDYRDIEDDRKHPVKNKRPLASGAVSRSAA
                     IIICIALMLGGFLIAWWIRDKFLFVLGIYFLINLGYSFGLKSIPILDIILLAIGFVLR
                     IKAGSVIAEIPLSEWIVIMVFLLSLFMAIGKRRDDVLLKISSGVDMRKSSKGYNLEFL
                     NVLLALVSAVIIVAYFMYTMSDDVIERLGTHRLYYTCLFVIAGIMRYLQIIYVSADSG
                     SPTKILYKDRFIQLTLLLWIASYISIIYMKDITLFK"
BASE COUNT         5133 a         3245 c         3372 g         4654 t
ORIGIN      
        1 tggaaaatga cacggtatta aatgatttgg ttaggcataa gttgatcgaa aaaagaaatt
       61 ataaaattgt tcattatgat tcaagggatt ttcggggcat tgacgttgga ctattgtata
      121 atcccaaata ttttatagta gaggcaagtg ataaactatt tgtacaactg ccgggtggtt
      181 caaaagacgc atattttacc cgtgatattt tatgggtaaa aggcaaactg ggaggtgaga
      241 ccattcatat ttatgtaaat cactggccca gccgcagtgg tggtgagcaa cgaagtgccc
      301 cggcaaggaa tgctgctgca caggtatgta aaaatcatat ggattctatt gctaagctgg
      361 aacctaatgc taaagtgatt gtgatgggcg acctgaatga tgacccaacg aatgaaagtg
      421 tggaaggcat actaaaagca aaaggaaaag aaagagatgt aagggcaggc ggcatgttta
      481 atccctggat ggatttgtat aaaaaaggta ttggcacact ggcttaccag gatgcatggg
      541 gtctttttga ccagatatta atttcacatg cctggctgaa taaggatcaa actggtttct
      601 ttttttacca gcagcatatt ttcaataaag aatacctggt cgaaaacaaa gggcggtata
      661 aaggttaccc gatgcgtacc tgggatggta atacttatcg tggtggctat agtgaccatt
      721 tccccaccta tcttattatg ctgagagaag taaagaagga aaagaaagga ttttaaaatt
      781 ttacactcaa aatcaagtat ttatctcttc tttacgattt gaaggagttt aaaaacttct
      841 tctaaaagtt cgttatactt ctggcggtaa acccttatct ttgccgcccc aaatctgtgt
      901 tcgcagattc tttgccggca tttccggtac tgtccaatgg gataaatcga atttttaaac
      961 ttaaaaacag gtaaatgaac aattacgaat tgatggtgat ttttacccct gttttgagcg
     1021 atgatgagtt caaagctgag caaaaaaaat atgcaaagct agttaccgat gctggtggct
     1081 ctatcgtagc tgaaaacccc tggggattga aatctcttgc gtatccgatt cagaaaaaga
     1141 ctacaggtct ttactgggtc atggagtaca ctgcgcaatc cgacttcaat gagaagctga
     1201 aaatccagct tctgcgtgac gagagtgttc ttcgtcacct ttgcactaaa ctcgataaat
     1261 acgccgtcga gtataatgcc agaaagaaaa gtggtgtaaa atcaggaacc gaaaaaacag
     1321 tggaggctta atatcatggc aaaaaatgaa attaaatacc tgaccgccat taaaagcgac
     1381 aaaagagaaa agaagttctg ccgctttaaa aaatatggca tccgttacat cgactataaa
     1441 gatgtagagt tcctgaaaaa atttctgaac gagcaaggga aattattgcc ccgccgcctt
     1501 tccggaaaca gcctgaaatt ccagcgcaaa gtttctgatg cggtgaaaaa ggcccgtcag
     1561 atggcattat tgccttacgt aacagacctt ttaaaataac ctcaccctaa cctcttacca
     1621 ttggtaagag agacagtatc gcaaaacgaa actgggttgg tgaacaaaaa gagaacaatg
     1681 gaaattattt taattcaaga tgtagataat cttggtgcta agaatgaagt agtaaaagta
     1741 aaaaacgggt atgcccgtaa ttatttgctg ccacgtcaac ttgcagtaga gaacagcccc
     1801 agcaataaaa aacaactgga agaaagattg aaacaggtgc gtaagaaaga agataaaatg
     1861 ctggctgaaa tcaatagcgt tattgctaaa ctgaatgaag cgccattaaa actgggtgct
     1921 aagacgggta caagtggtaa aattttcgga agtataactt ctcttcagtt gagccgtgct
     1981 attcgtgaac aaaaagggta tgaaatagat cgtaaaagaa tctccatacc tgaagatgta
     2041 aaggagatcg gcacttataa agccactatt gactttggta atggtaaatc tactgaagtt
     2101 gagtttgaag taatagctga ataaaagagc tgaatattct taaaaaagtc ctcctttttg
     2161 ggggactttt ttattgcctg ccctaatctt gactgctcaa aatcaataat ttagcttcaa
     2221 ctacctattc ccaaaagaat aatttggtaa tatctaagga aaaaatccgt tattttgtct
     2281 tgcttttggt gctctttcag tttcacaagt cctgaacgat tcgaaaaacc gtagaatgtt
     2341 cattgggtac tgtttactgt tgacactttt atttttttta aaacctctca aaatgaacat
     2401 ttacgtagga aacctcagtt ggaacctgaa agatcaggat ttatctaacc ttttcgcaac
     2461 acatggcgaa gtagcatctg ctaaaattgt aacagacaaa ttcactaacc gtagcaaagg
     2521 ttttggtttt gttgaaatgc ccaacgatga ccaggcgcag gcagctatag ctgctttgaa
     2581 tggtactgaa gttgatggtc gtaacatcgt tgttaatgaa agtcgtccga aacaagaagg
     2641 cggcggcggc ggtggcttca agaaaagaag cttcggcagc ggcggcggcg gttacaaagg
     2701 cggcagcggc ggcggtggcg gctacaaagg taagagtggc ggcggcggcg gcggcggtta
     2761 taaccgtgat cgtggtggat acaataacga ttattaataa taacgatatt gacgaagctt
     2821 taaaagtctg gttcggaaga gccggacttt ttttataaca ctcattactc cgctttttta
     2881 actcccccgg ccccacttac ttgctggctt ataaccggat tacctttata ctttatatca
     2941 gcagcacctg ataccttgac atccagttta acactggcaa acacctgtgc atcgccggca
     3001 cctgataatt ttacattaac attttccgct aacagttcat aacatttaat atcagtgctg
     3061 ccagaaccat caacggtaaa gtcctttgtt tcacctctca aagtaatagt gccggctccc
     3121 gataattccg catcaactcc gggtgctttt aactccattt taatatcact ggcaccgcta
     3181 aggtcaatgg aaatcttctc atcactggtt atcttattct caccgaagat atcacaggca
     3241 ccggaagctt taaaatgcct gaaagcattg ccactaacat atacctttat agatcttgaa
     3301 ggttttaagt tgataccttg cttttgatga atctccaggg tgccaccatc attttccaca
     3361 attacatatt caagcaggtt ctcatcggct tccactttta cagaagtgnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ctcccgaacc tcacacagtt ttctttacct
     3541 tcgcagcacc atgacggaaa agatactcat cctcgatttt ggctcgcaat atacccagtt
     3601 aattgcccgt gctgtaaggg aagccaatgt gtactgtgaa atcattcctt attacaaacc
     3661 atttgaattt gagcctggac ttaagggtat tatcctttca ggctcaccct tctcagtaaa
     3721 tgaagaaaaa gccccggatg ttgatgtcca aaacttcatc accaaggtcc ctgttctggg
     3781 agtttgttat ggtgcccaac tgactgccaa acgatttggg gggatagtgg caaaaagtaa
     3841 taaaagagaa tatggccgtg ccatattgca atgtaaaaaa gaagatgttt tactgaaaga
     3901 tgtatcccct caaagccagg tttggatgag tcacagtgat actatcaaac aattacccac
     3961 aggatttgaa ttactggcag atacagagag tattccggtg gcagctttta aaaaatcagt
     4021 aaagggtact cccgactttc cgctctatgg ggtacaattt caccctgaag tctatcattc
     4081 taaagaaggt aaaaaaatac tcggcaactt cctggtaaat atttgtggtt gcagccagga
     4141 ttggaccccg gcacatttta ttacggacac tgttgaagcg cttaaaaaac aaataggcga
     4201 cagaaaagtt attatggcgt tgagtggagg agttgactca accgttgctg caacattaat
     4261 tcacagggcg atcggagaca ggttattcgg aatatttgtc gataacggtg tcttacgcaa
     4321 gaatgaattc gagtctgtat taaaaaccta tgctcaactg ggccttaatg taaaaggcat
     4381 taatgccaga gaacatttct ataccaagct tgccggaaaa accgatccgg aagaaaaaag
     4441 aaaaattatt ggaagcatgt ttatcgatgt tttccaggaa gaagcaaaaa aaattgaggg
     4501 ggtcggcttg ctcggacaag gaactattta tcccgacgtg attgaaagtg tatctgttca
     4561 cgggccatcg gtcactatca aatcccacca caacgttgga ggcttgcctg atcacttaca
     4621 tcttgaactg gtagaacctc ttcgctcttt atttaaagat gaggtaagaa aagttggccg
     4681 tgaactggga atacctgctg agatgattga ccggcaccct ttccccggcc ccggtctcgc
     4741 catcaggatt ttgggagaaa taacagaaga aaaggtacag ttattgcagg atgcagatca
     4801 cttatatatc caggcgctaa aggataacaa tttgtatgca actgtttggc aggcaggtgc
     4861 tatcttactg ccggtaaaaa gtgtaggggt aatgggtgat gaaagaactt atgaatttac
     4921 agtagccctg agagctgtaa ccagtgtaga cggcatgact gcagattggg cacatttacc
     4981 atatgaattt ttagctaata tttccaacga gatcattaat aatgtaagag gaattaaccg
     5041 ggtagtttat gatatcagca gtaaaccacc agccaccatc gaatgggaat aatggcagag
     5101 gctaaatata tatctatgag aatttatctt ttatcagtat ttgcatgcat cgcttttttc
     5161 caaaatatac atgcgcaagg cgacacgacc aaatacaaaa ttgcaatctt cgcaccactt
     5221 tatcttgact ctgcgtttga tgcagctaat gagtatcgct atgcaaaaaa tgtattcccg
     5281 aaatttataa acccgggtct cgaattctat gaaggtgcgc aacttgcact tgactcactg
     5341 aataaagaac aggcccccct cgaagtacat atattcgata cccgttccgg taaagaaaca
     5401 atcgccgaac aattagccaa accggaatta aaaaatattg aactgatcat agcctattgc
     5461 tcggcagcag aagtaaagat ctttgctgac gccggtttac aaaaaaatat acctgttata
     5521 aatgttaatc ttcccaatga cggaggtgta acagctaatc ccttttttgt tctcctgaat
     5581 tctactctta aaacgcagtg cgaaggaatt tacaggcata tacagaaata ctattcatta
     5641 aatcccctca ttgtatttcg caaaaaaggg caactggaag atcgaataaa gacctatttc
     5701 gatgactttg gcaaaagcac tttatcaact ccattaaagc taaaatatat tgatctgact
     5761 gatagtttca ccgttaatca gttgaggcct tacctggaca caatcaaccc cactttatgt
     5821 attgccggca gcctcgatga gaattttggg aaaaggctta cgcagcaact ggcctcattg
     5881 acaaagctga aatataaagc tgcagtaatg ggaatgccta cctgggatgc gatcaaagat
     5941 tttaataaac ccgattataa ggggattgaa attatctaca gtaccccttt ttacaattcc
     6001 cggactgata aagtcagcca aagcatcatc gaacatttca atactaaaat gtatgcccgg
     6061 ccaagtgata tggtgatgcg gggatatgag acggtttggc gttttgccaa actactcatt
     6121 caatataaat cagattttgc ctccaacctc accaggaaag attacagtgt attcagggaa
     6181 attgatatac agcccgttct gaataaacaa agcatgacac tggactattt tgagaataaa
     6241 aaactttatt ttctaaaatg gatggatgga gtgatcaaag cagttaacta aaaactgtat
     6301 tttcatattt cattttaaaa aggggaattt tcatatccct gccccctcct gcagacgtgc
     6361 aacttacaaa aggttatact accattacag attggctaga aggaaaagga ttcaagcctt
     6421 ttttatttca ggagcaaacc tggcaacata tcatcactgg tgaaagcggc cttgtaaatg
     6481 cccctactgg ttgtggtaaa actttttccg ttttccttgg ttcgatcatt gattttatca
     6541 atcgtcatcc ggctgactat aaaaaaaaag ctggcagcgg gttacagctg ctttggatca
     6601 cacctttaag agcactggcc aaagatattg gcagagcaat gcaagaggtg attgaagagt
     6661 tggacatgtc ctgggaaatt ggtatccgga atggtgacac ttctactgcg gaaagaacaa
     6721 aacaaaaaaa aagaatgccg gaagtgctga tcatcacccc ggaaagtctg catttattac
     6781 tggcacaaaa agggtacccg gatatattta aatcattgaa aataattgcg gttgacgaat
     6841 ggcatgaact gctcggaagc aaaagaggtg ttcaggtgga attggcaatc agtcggataa
     6901 ttgggtgcgg gaattcgggt gttagtatct ggggaatcag tgctacaatt ggaaatttag
     6961 aagaagcaaa agaagtattg ttatctacac ttaagcaaaa cggtgtcatc gttaaagcaa
     7021 ctattcaaaa acaaattgag gtagaatcca taataccaga tgaaatagaa aagtatccct
     7081 gggcgggtca tctgggtata aaactggcgc ataagatcat tccaatcatt cagcaaagcc
     7141 gtactacact catttttatc aatacaaggg gcatgagtga aacatggtac cagaccctgc
     7201 tggatgtctg cccggatctg gccggttcca ttgccctgca tcatggatca attgatatgg
     7261 aactacggac atggattgag gataatcttc ataccggaaa cctgaaagct gttgtttgca
     7321 ctgccagtct tgaccttggc gttgattttc gccccgtcga aacagtgatc caggttggtt
     7381 ctcctaaagg agttgccaga tttttacaac gggctggccg cagcggccat agtcctgatg
     7441 ccataagtaa gatctatttt cttcctactc attcgcttga attgatggaa gcggccgcat
     7501 taaaaaatgc tatgacaacc ggtataatag aaagccgtca accaatgctg ctttgttatg
     7561 acgtattact ccagttcctg aatacactgg ctatctctga cggatttaag ccagaaattt
     7621 tatatgatga aataactgct acttattgtt acagagagct cttaaaagag gaatgggata
     7681 atatacttca ctttcttacg gaaggaggct tagccctgca ccagtatgat gattataaaa
     7741 aaattgaagc agaggatggt ctctataaaa taaaaagtcg caaagtggcg atgcggcatc
     7801 gtcttcatat tggtacaatt gtaagtgatg ccatgatgaa agtaaaattt atgaccggtg
     7861 gttatgtagg ggtgattgaa gaatatttta tctcgaggct cgaacccggt gatgtattca
     7921 ctttggccgg aagaaatttg gaatttgtgc tgataaagga aatgaccgca ttggtaagaa
     7981 aatcagcaaa aaagaaatct atcattccaa gctggactgg cggaagaatg ccgctgagtg
     8041 ctaacctggg taaaaaactg cgtgaaacat tagaccagtc cggggacaag aaccggatgc
     8101 caggagtcag ggaacttgaa gtactgtcac ctctctttga attacagaaa aatctttctc
     8161 atgtaccaaa acaaaatgag ttgctgattg agaagataga aacaaaagat ggttttcatg
     8221 tttttgttta tccattcgaa gggagattag ttcatgaagc catggcagct attctggctt
     8281 accggatcag tcgtatcaga ccaattactt tctcgtttgc catgaatgat tatggttttg
     8341 aactgctgag tgatcaaaca ataccactgg atgacaagaa tgtttatgca ttattctcgc
     8401 cacaaaattt actgaatgat attcagcgaa gtgttaatag tacagaaatg gccaggcgaa
     8461 aattcaggga tgtcgctgtt atcgggggtc taatattcca gggcatgccg ggagaaaaaa
     8521 agaaagcccg gcacctgcaa tcgtcagcat ccttattgtt taatgtattc agcgagtatg
     8581 atcctgctaa cattctactc cgtcaggcat accaggaagt attggaccag cagatggaag
     8641 aagcaagact aagggatatg ctgcaacgga tacagcaatc caacatcatc attacacatc
     8701 cgcaacaact tactcctttc tgttttccaa ttaaagtaga tagtatacgg gaagaccttt
     8761 cttctgaaaa attggaagac agggttaaaa agatgcaaca acaactggaa aagggagaat
     8821 agtttttact gtagatttgc cattccattt tcaatgcaaa accctgttcc ccatattatc
     8881 cgtaacaata cattctgggt atcaccggaa cgctgtttgc tatgggaaga agagaatacg
     8941 ttgatcgttg ctgacctgca cctgggcaag tcaggccatt ttagaaaatc tggtattggc
     9001 ataccacaaa gtatttataa agctgatctg caacgattga tggcacagct ttacctgtat
     9061 aaagtggaca ggctcattat tgccggtgat cttacacata gcacagctaa taaagagctt
     9121 gatttatttt taaaatggcg aaaggacttt tcattgttac atattgattt agtaaaaggc
     9181 aatcatgata ttttggctga cagctggtat gaagaagcag ctattaaagt aagcacctgg
     9241 aagatgaaaa ccgggccatt cattttctta catgatttga aagcggaaaa agacttaact
     9301 gcagaggaaa aaggattata ccgttttacg ggtcacttac atccagccat aaacctcaaa
     9361 gggaagggaa aacagtcgct caagtttccc tgtttttatt ttacaaaaga gtattgtata
     9421 ctgcctgcat tcagtcgttt tacagcaggt tttaaagtaa atcctgaaaa agatgaaact
     9481 gtgttcgcca ttgtggaaga tggcatcatg aagctaccct agcgtttctt acgcagctgg
     9541 ttataatcaa gaaagtaaat aaagcgtaca gtaaattcat taccgtggcg aaggtcgaat
     9601 gtattcccca gatttttcag gtacgtattc ctccccgtac cgatgttaac catttcttct
     9661 tcacccagcc agtttttata tcccagtatt aaacggctgc cgagacgaaa atcccaggta
     9721 agaaaagcat cgacattgaa tacattgaaa ttctcatctt gcccggggat aaacaaccgg
     9781 gataataaat atcccttatc atcaacgtta tgaaaactaa tatagttcac cctgttccag
     9841 taatgccttg cccgaagtgt cagattaagc cggtaagcaa aattgtatat accagaaaga
     9901 acagaagtaa agtcacggtt atcacggaaa ccaacgatcg gttccccgtt cacttcccgc
     9961 ctgaaggcat atcccagttg gttctcttca aagtgggaat cagtttgcag atccagtgaa
    10021 aacttatcgc tgaaacgata gcgcatacca aggctgatac cggtataaac attattatat
    10081 tttggagcta gtgcataagt acttccaaaa cgcacaaaaa ctcttttgcg gctatccgta
    10141 cttccctcca cctggaaaat ataattggca ggatagctaa gaggacgacc atcagttctt
    10201 aattcaaaat aatttcgctc ccatcccgga atcacattgg aagtgagtgt aacatcccag
    10261 aaatttctga atatccagaa tgcagttgcc gttacatcaa atttgctata tgcatagggt
    10321 ttatacaggt attgcagtct tggcgcaata gaataactgt aattaataaa gtttttagta
    10381 gggacaaact tcctgtaaga gaggttccca cggtaagata tttcattggc agctaacaat
    10441 atccccagat cattgggatc atagtttttt gattccacat ttccctgtac ataatattgc
    10501 caattgccgc ttactttacc atacttcacc gtagtattgt atccatcata aggatcagtt
    10561 ccccatattt tgctgtaacg ggcagttccc tgaaatgaat acatattttt tttatcatat
    10621 aaggcaagat ccaatgctgt tacattagca tctcttgcat cgccattacg cattacattg
    10681 gtattagtaa aagtgagtga agagcggcct ttgaatgcct ggtcaagcac aataatacta
    10741 taatttgcca gggactcggt ctcaatcttc gtctcttcat ttgttgttct atccctgata
    10801 atggcatgta taggtgcagt aactgcatta aaaattccta tacccaattt tttcttggtt
    10861 cgtcctgaga atttgattgc attatacaac tgtgtccgtg atggattttt cacgatttca
    10921 atgtttgggt ctgcatcagc aattcccagt acatcataat aaccacctgg cattgcccct
    10981 acccttcggg aataaaaaag tttagcctta ttaaatatct ccgttccttc tgtaaaaaat
    11041 tgccggtttt cctggaactg tacttcgtag ggtgtaaggt tattcactac gttatcagat
    11101 attacctgcc cgaaatcagg gataagtgta gcatccagcg taaagctttc gttgatgcca
    11161 tatttaacat ccattccccc atttctcagc cattcattca gataagtatt accaacggga
    11221 gtagttctgt aaccagtggt tacataaggg gaaaaactaa gcctgagagg tggaacgata
    11281 ttttgcaaat tggtaaatgc accaaactga ttgacaaaac cattgacttt tgggttgaca
    11341 ggactccaga aattcgattc attattgcga cgcatgaacc ggagcaattg aagtccccag
    11401 tcctgaacat ctttttttgc aaagcggaga gaaatataag ggatacgcat ttcaacagtc
    11461 cacccatccg gttgcataga caccttactt tcccagaccg catcccatgt tttatcacca
    11521 taactgccaa aatcaccagt aaaactgggg gtaagcctcg catctgtttg cacatttgca
    11581 gttgttacga ggaactgaaa accgttctgc ttatcattat aagtatcaaa gaagatagaa
    11641 aagtagtcaa catccgtctg ttgttcccca tcacgggctg taaactgttt cctgattaaa
    11701 gcaggatcat catataagta cgccccaaca taaatagcag tattgtcata aacaatccta
    11761 acctccgttt tttgggaaca gggcaatcca aaagtgggtg aattttggat aaaatcaccc
    11821 aaaaccggcg cattggccca caccccatca tccaatacac cgtctatttt tggtgcctgg
    11881 gttactttta ccgccactgt tgttttaacc tgcgaaaatg attgaaaaca aaggagtaaa
    11941 aagggtatgc agatatagtt tttaagcctc attcagctgg ttgccatcta caaatataat
    12001 agacaaaatg caaggataaa aggctgccgc agaaaaacgc aaaaaaagct atccctcaat
    12061 agtaaaatga ctaattcatc ctctttttca atgaagttac ctgctgttgc agatcggata
    12121 cgacattggc caactgggcc atatcccagc gattttgggt attagccctt gaaataacaa
    12181 cctgggcttg ccacatttcc agtacttcct ctgcgtttac cgtgtaggga tgaaaggaag
    12241 gattatcact gacaagtgtc aatttattct tcaaacggcc atttttctgc acccttttat
    12301 aaacaattcc ttcgttgcgg gagataacaa tacaagcggc attattcttc acttcatcca
    12361 ggttgtccac tttctcccct actattaccg aaccactggg ggttggtaac atagagtcgc
    12421 caatgatctc aaatgcacga taattaccgc ctgttagcat cggcagggta aaggtgttca
    12481 actcgtcaat gaactcggga tcagcataac cagctaaata gcctgcggca gctttaaccg
    12541 gtacaaaagg aatatcaggc ctgccactgg caagtttcaa tgccctgcgt ttggcgagat
    12601 agttactttt attatcactt aaatccttcc ggagaatatc atccagcgtc agtttaaaaa
    12661 tatcacatac cacttcgagc acatcaatcc ttggctcagc cctttcctcc tcataagctc
    12721 ccagcaatga acgcttgatg cggagtttgt tggcaaattc ctcctgtgtc cagccacgca
    12781 gtttgcggag atatttcaga ttttgattgg aaatagccat aacatctaat ttatttagct
    12841 gcaaaatact aattatttta gattttcaaa atagtttata gctgtcattt tggttaagcc
    12901 ggaaaaaaca aagatttttg ctatgtatga aaaagaaagc cagccccact cctaagaaaa
    12961 attctccggc aaaaaagaaa gttgctaaac catcagtaaa agaaaaaccg attattctga
    13021 taacaaacga tgatggtata actgccccgg gtatcagaaa cctggtagaa gccgtaaaag
    13081 acattggaaa aattgtagtg gttgcccctg ataaaccaca gtcgggcatg ggacatgcca
    13141 ttactattgg acagcctctg cgtctccata aactggaagt ttttgatgga gtggaatcct
    13201 atgcctgcac aggtaccccg gtagattgtg taaaactggc tgtagataaa gtgcttcacc
    13261 ggaaacctga tctttgcctg agtggtatca accacggtgc taatcatagt atcaatgtca
    13321 tttattcagg tacgatgtca gctgctgtgg aagcagcgat agagagcatc ccttctgcgg
    13381 gtttttcatt acttgattac agtatagaag ctgactttac aggtgccaaa aaatttgcaa
    13441 agctgattgt tgagaaaatg ctgacaacca aacttgacaa gcataccgtt ttcaatgtca
    13501 atgtgccttc catacatgtg gacctgctaa aaggcataaa gatctgccgg caggcatatg
    13561 ccaagtacga agaagatttt attgaacgaa atgaccctca tgggcggcat tattactggc
    13621 tgaccggtga atttgtaaac tttgataaag ggaaagacac tgatgtgtgg gcattagcaa
    13681 acaattacgt aagtgtggtg cccgtgcaat ttgatctgac gaattacgtt ttaaaggcca
    13741 aactggaaaa aagctggaaa taatgatact tacaaaagac aacctgaaac ttggcctggt
    13801 acttggtttg acggggccta ttattggcct ggtagccatt tattttttaa aattctcctc
    13861 ttttactttt atagaattcc tggacaattt tatacatgaa aacaggctga ttacgtcaat
    13921 tggttcactc agtctgctgg caaatgctgt tctttttacg atctatgtga atacgcatcg
    13981 ggataatacg gccaaaggaa tttttattgt tactcttatt tatggcatag gcattttggt
    14041 gctgaaaatt tttaattaat ccttattaag agctgtcgat gaaatattac atcattgcag
    14101 gcgaagcatc cggggatctg catggcagca atcttattaa agaactgaaa aagcatgact
    14161 ctgctgcaca gatacgctgc tggggtggtg ataaaatgca ggaacaaggt ggggaactgg
    14221 taaaacatta ccgtgacctt gcctttatgg gttttgcaga agtactgcta aatctccgga
    14281 ccatcttcag gaacctagcc ttttgtaaag aagatatcca gcaataccaa ccagatgcaa
    14341 tcattttaat tgactatcct ggttttaatc tgcgcattgc caaatgggca aaaaagcaaa
    14401 agcatagagt tatcttctac atatccccgc aagtgtgggc atggaaagag aaccgggtaa
    14461 aaatgatgaa agaatgtata gacaaaatgc ttgttatact gccttttgaa aaagattatt
    14521 tcaaaaataa gtggaactgg gaggtagaat atgttggtca tccgttagtg gaagtaatag
    14581 aaacccggtt atcggaatcc ggaaatgaaa aattttctga taacccaatt gtagcattac
    14641 ttccgggcag tcgcaaacaa gagattttga aaaagctacc ggtaatgctt gaagtgagta
    14701 aatcttttcc cgcttaccag tttatagttg ccaaagctcc cggaacagat gaagcttttt
    14761 acaatcaact ggtgaaggat tattccaatg tttcctatgt gacaaataaa acctatgacc
    14821 tgttgctcca gtcaaaagca gccctggtta catcaggcac ggctaccctt gaaacagctt
    14881 tatttaatgt gccggaagtg gtttgttata aaggttcctg gctttcttac cagataggca
    14941 aaagattggt gaaagtgaaa tacatttcat tggtgaacct gattatggac aaattagtgg
    15001 taaaggaatt gatacaagct gatctcacgg tagaaaaact caaaacagaa ttaagcgaac
    15061 tgttgaataa tgaaaacagg atcagggagg taaaaacaga ttattccctg cttaaacaaa
    15121 tgctgcacaa agatggagat gcttccgcaa aggctgctgc ctccattttg caatttctgt
    15181 caggtaagta atttgactgc cagaataata aggcatagta taaaaacaaa aatgctgatc
    15241 cggttaatac catgcatata cccgatccat ttatcctttg gacggttcgg gtcttttttc
    15301 ttcaggaata agtattcggc cagttggttc cacactccca taaaataatt tcattttaaa
    15361 gtatacagat tttttaaaat taagtttaaa aacaaataaa aaaaattgca gaaaaccaaa
    15421 taatgccaac ttattaaatc ccttttttgt agatttccga ttgcattttt cattcataac
    15481 atatagcttt acaccagtac cgcaaaacca ggccatggct tatattaaat tactacgccc
    15541 aaaagattgg gcaaaaaatt ttttcctttt cattcccctg ttctttgccg gagaactttt
    15601 caatatggag aaaataattg ccattttatg gggatttctt tctttctcct tcatcgccag
    15661 cagtatttat attatcaatg attaccgcga cattgaagat gaccgcaaac accctgtaaa
    15721 aaataaaaga cccctggcat ccggtgccgt ttccaggtct gcagctatca ttatttgtat
    15781 agcattgatg ttgggtggat ttctgatagc ctggtggatt agggataagt ttttattcgt
    15841 attaggtatt tatttcctta ttaacctggg ctattcattt ggccttaaat caattcccat
    15901 cctggatata attttattgg ccattggatt tgtccttagg attaaagccg gatctgtaat
    15961 tgccgaaatc cctttgtctg aatggattgt gatcatggta tttctgctct ccctctttat
    16021 ggcaattggc aaacgccggg atgatgtttt gttaaaaata agttctgggg tagatatgcg
    16081 taaatcttca aaaggatata accttgaatt tctaaatgta ttattagcgc tggtctctgc
    16141 tgttatcatt gtagcctact ttatgtacac aatgtcggat gatgtaattg aacgactggg
    16201 cacccaccgg ctttattaca cctgcctatt tgtaatagcc ggtattatga gatacctgca
    16261 aatcatttat gtatcggcag actccggctc ccctaccaaa atactctata aagaccggtt
    16321 tatccaactc actttactat tgtggatcgc cagttacata tcgatcattt atatgaaaga
    16381 cataactctg ttcaagtaat gaaaaaggga attgcattct ttgattttga tggtactatc
    16441 accaccaaag acacattact tgagtttatt aaattctgta aaggcaaaat acatttctat
    16501 gccg
//
LOCUS       DAORTI010000076        16232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3314_length_16232_cov_5.690610, whole genome shotgun sequence.
ACCESSION   DAORTI010000076 DAORTI010000000
VERSION     DAORTI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_16232_cov_5.690610"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLO70_10430"
     CDS             <1..272
                     /locus_tag="PLO70_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74928.1"
                     /translation="QDVSKYVGCAFLMQLATPRRTGRGGIFMYGTVFHQGTVSIKNIT
                     LPVYDMWAFSIHAKFQIPYVQFVSNLNITFKFEEAEYSVPGYVWW"
     gene            324..875
                     /locus_tag="PLO70_10435"
     CDS             324..875
                     /locus_tag="PLO70_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74929.1"
                     /translation="MKGFFLVHFLIYLFSCATHSQGGGKEFIVNEKLKNKFSDGPLPG
                     VRGGPDRVYLNGVLVDSTYIKSDIHFVYNASIRDINGNHLPFDTCTSFFDGDTLMFVF
                     KEMNEPSSDKIEVRIINKTYSIIFTSNNSNEGIIASNESLILNSDLGIRKRVVLGDIA
                     FLVNDKVNNKQYSFKGSFICKVE"
     gene            1211..1801
                     /locus_tag="PLO70_10440"
     CDS             1211..1801
                     /locus_tag="PLO70_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74930.1"
                     /translation="MPVRTNMPYNTGLYFITFTCYKWLPLIEQTRSFDLVYKWFDYLK
                     TQGHSITGYVIMPNHIHALIDFAVSAKKLNTVIGDGKRFMAYDIVKRLKEREETETLM
                     LLENAVQSKDKANGHRHEVWKESFDWKYCETATFAYQKLLYIHNNPCNGKWNLASDIT
                     KYEHSSARYYISGRHAGYEVKDVEEVISKLTQGRAE"
     gene            1891..2382
                     /locus_tag="PLO70_10445"
     CDS             1891..2382
                     /locus_tag="PLO70_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74931.1"
                     /translation="MTSTQKDVLALGCIGVVMLELGITGNPSLLGGYKNFNKALKGAK
                     ELQKMIDNNPEAYAENARVVLYGFSFQDDNDTYKFDEDGKLNLTEAMLYEIDAEAEKN
                     KRGTNFDFGLYNHRTLTIFDADGKEPGMTVYERTIEGFEAIFGNKHAYYYQITTVPIK
                     LKF"
     gene            2415..2735
                     /locus_tag="PLO70_10450"
     CDS             2415..2735
                     /locus_tag="PLO70_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74932.1"
                     /translation="MFIFFTAVSNKHEGNFNQKRITVIGKAVVMKHYAAVRTDDSVFY
                     FLEGKDDWDDKHLGKRVKVSGRLYIKEWHDAKKSENPEITAIPQQRYGAWKMLKKPRW
                     SLVE"
     gene            complement(2753..3442)
                     /locus_tag="PLO70_10455"
     CDS             complement(2753..3442)
                     /locus_tag="PLO70_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74933.1"
                     /translation="MKYLFITSLFTVMLTCSFSSCTGNREKKETLSSKDIVVDSNAVL
                     TYTELDTFNNATNKIEKLLDLPSLKETQNDSEIRIWFEYALSDSGKLIILKSEHNNWI
                     SHAIYYKMKFDEMYKIVSVSKKEETNEPNSGWKNFIRKINGLGIYDLKNYKEISGYSV
                     CADGDPLTIEVKKDGKYKLLQYPCWEVYVIEDPLAEIIKIKEICLLIQKEFGYKLFPR
                     PHSASVLDQIK"
     gene            complement(3453..4322)
                     /locus_tag="PLO70_10460"
     CDS             complement(3453..4322)
                     /locus_tag="PLO70_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74934.1"
                     /translation="MRAIMVNEDNFFGGEMSGMDSKKFRQQWKADKEWQISDAEILWD
                     QIIDFAGSGGSIISITGASATEYKDFSFSFNEGELAGTYGSISSATFSYDGYDYYTSS
                     DIAAFAWARKEGYSLGIRDAAEKETEWASLIFEKIDKGTGVRYYSYTKPDKFKTFLKR
                     YGSPGPDADPKKFHGKLPKGATLYGYIHLHWEGSEYLDPSNIGFSRSSGGGKGDMLKM
                     DDFPNLKFYVVGSTGILWARNPAKMAPNRAEWPTDTDAKKIYRVAENVYGPGAMTNLI
                     PLTFKDLIPCKLE"
     gene            complement(4405..4854)
                     /locus_tag="PLO70_10465"
     CDS             complement(4405..4854)
                     /locus_tag="PLO70_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74935.1"
                     /translation="MVKFLKNITNGFLSILFIFLYSIICETSNSITLEYGWLTDKPIP
                     KIIFYADTFNKNESWPYYAYKVNRECLKLIKNEIKGFDSVGILKDTNQVGYYNYVICT
                     QSQKTRYITDNKKHTKKLLNKILDRINDTAVKRSVGLRFDDISRRIW"
     gene            complement(5097..5888)
                     /locus_tag="PLO70_10470"
     CDS             complement(5097..5888)
                     /locus_tag="PLO70_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74936.1"
                     /translation="MKKVLFLFLIFFLGQRCISQDDFNKEYWLIRANIEKPYETLLRH
                     TIVSPKQSEYKIDSFFIDHLIRKYSGPGNLKYFISNRFFLERFNYDFLLHDKAALQIA
                     IDTNKFRIEAKKISKSLSSLGKIQYDSTVFNTVHSMDGYTYILSINSKEIFGLTWDTP
                     TVSVLDSLTISINDKRFIIPDSIFSDLYFPNFCEISPNKRPIEAFLSPNKKYIYLYVT
                     GGQMNVNFFCKIIFDIEGRKVVGRMIADNNELSWYRTFNKNFIGF"
     gene            complement(5894..6712)
                     /locus_tag="PLO70_10475"
     CDS             complement(5894..6712)
                     /locus_tag="PLO70_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74937.1"
                     /translation="MNEEQGGFQQLTGFERSGQDWSSHEQFFDWDEQLTQILTAAFIR
                     AYMASISEKLGSGGSGSNIKGGGISIYGFGSNETPFVWTPGKAYSGKSTAISETVAVL
                     EELYKNGGILQFSTIGDNTVKNVSGNVLCDFLEGGKFSNVPITIYAGMENTETKEDGS
                     IWFDNREGLEILSYTDPVSGITQKVGYQNALMGLVHELGHVWLRTAIPLYQYERLQRL
                     PFLNPMGNPFLEHIILKNLERGNVPQVFGQAERIFYYKVTLFKTDGPFSIKKIN"
     gene            complement(7007..7288)
                     /locus_tag="PLO70_10480"
     CDS             complement(7007..7288)
                     /locus_tag="PLO70_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74938.1"
                     /translation="MFLSINTSNQTYNSSRPKAFINWILFDEQFKYVSAGSGFEQVGA
                     SDTFTTHLFNDVAINKSGYLYIYVSNETPNIDVFFDNLQVTHVRGALLN"
     gene            complement(7374..7811)
                     /locus_tag="PLO70_10485"
     CDS             complement(7374..7811)
                     /locus_tag="PLO70_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74939.1"
                     /translation="MYKLLIIALVQFYTLIGFENYNNYGGTIILEYAGPQNRPLTTLV
                     FYVDSFQKLTSPYFKAFKINRPEFEAIVKVVSSDSVLLIKPEQGLGYYNFTVKDSSAD
                     VRYVTKDKETTKRILFEITKTMTNLILREKVESEINSICKELW"
     gene            complement(7845..8696)
                     /locus_tag="PLO70_10490"
     CDS             complement(7845..8696)
                     /locus_tag="PLO70_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74940.1"
                     /translation="MRAIAMNEEQGGFQQLTGFSRHGHDWNSQNKLQRSSLIGKAIEA
                     YWNDIFDRLEQYNANGSGGYDFQAVVDGHVNGITGISINSGIEYKEGHTINVMEGGLL
                     VGTIYVNRFRANPSEILFKDGTRTFAELLMINLLFRNRSARYRDFEWIHSITTNDPGA
                     CNVNQFVDPCDAVPFIPRSSYPFYHSDQEIKDGVNIANGFDTEFFDTPGREPVIGMTK
                     TMDFELTLMAWEEKKGSYKPLVTLNYGLVVTKDANGSYMSIKNITIGSPSGFQQNLIN
                     YAQIMRR"
     gene            complement(8706..8984)
                     /locus_tag="PLO70_10495"
                     /pseudo
     CDS             complement(8706..8984)
                     /locus_tag="PLO70_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(9119..9625)
                     /locus_tag="PLO70_10500"
     CDS             complement(9119..9625)
                     /locus_tag="PLO70_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74941.1"
                     /translation="MKLIAFSILSVLLFECTGSQNKVPDQSADKKDNILACPDIDTAL
                     LEYVKDFDPRAIDLDESLNPRLKQLMDTIDTTCLRRQKQYKYFIISIVAKLYRYQVTC
                     CKIGYNLIDMKEGAAKIIINEYQNLVGYPGKREFLNSGTVIAYIKQDSLLREDKMLKE
                     IIKQVGWD"
     gene            complement(9640..10851)
                     /locus_tag="PLO70_10505"
     CDS             complement(9640..10851)
                     /locus_tag="PLO70_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ74942.1"
                     /translation="MNEDHYYPFGLTQAGISSKALAFCGAANKYKYNGKEQQAAEFAD
                     GNGLEWYDYGARQYDALIGHWHVIDPLAEVSRRWTPYNYAYNNPIRFIDPDGMKAIAM
                     NEEQGGFQRLTGFDRIKGDWSKRYDSYFQKMEQDQALEMLIDNLKEMFASAGGSIDSQ
                     IDFEGHTSWSFSNGYSGYESTQSRGIYLSIAGQGNSPNYVGSENDVFKDRADRLAKSK
                     GFAPSIYVKSGKELLKALKVATSKYGSIAGIVFFSHASGQGLILSESNGFYSENHKNA
                     YNGGDAADVVDLQLMIERGEIKFDEGAAIIFGGCNCASPADFPIGTDISIGESIVRAI
                     GVTAYAANDQIQEEIINGKGTGGITSPGGNFIKMERVYRFIPGWGRGGYAIDIRQTDV
                     GKTVNPINILR"
     gene            complement(10949..11380)
                     /locus_tag="PLO70_10510"
     CDS             complement(10949..11380)
                     /locus_tag="PLO70_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74943.1"
                     /translation="MKFLLFICFLYCGFKSANGQIGIASHEKSISKIVVYYLPKDRGG
                     PIGITDSNIRNISITSKFEISDSLCLSEIQKNIVRLKKIKAGHVMRSIYLLCDIEYRN
                     GTKTSISFEKAQSGVTKFQGKMYENSPQLETWIIGCINKSF"
     gene            complement(11391..12251)
                     /locus_tag="PLO70_10515"
     CDS             complement(11391..12251)
                     /locus_tag="PLO70_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74944.1"
                     /translation="MKAIAMNEEQGGFQQLTGFSRKKENRKLGGDAMSHYWDGVLGDL
                     MNKLGGEGTSEGTDIFIGVVGGSAGILVNVSGSTTNSDGSKSNINQTLVFGQNTEGSF
                     GFFDSVTGIQYDAEKNNYVKELLSAFNYLISGGAGIEVLFLMASKEGILIEDAESASV
                     KGGLGPEFLPVYNLFNRSGHHKILWNPFAGSYVIKNNLVNGIQSPALLLLHEIGHANA
                     YMRDINSTNQRKTLPTWGPEIGDYDDFEEMRVIKYVETPAAIRLGEPIRTNHRGGLTV
                     VPNSIFHIKY"
     gene            12578..13429
                     /locus_tag="PLO70_10520"
     CDS             12578..13429
                     /locus_tag="PLO70_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQZ74945.1"
                     /translation="MREMTAERHYWKRFYKAFCDYLYDDIGCFDNYAGSQIKIIKAFF
                     NFLNKELTMGIGEFHRSFYVRRENIAIFPLMPEELNFLISNKHFEEQLSFRMKQVKDF
                     FVFGCTVALRVSDLFALRTSNIRIVNGMYYLTVRSQKTSVHTHIKLPEYAVNIINRNT
                     QNSRLLPHFSSCNLNRSIKKLLELAGFNYPVQLLREKRGKVIELCSSGQSGIRARFCD
                     VCASHTMRRTAITTMLSLGMQEQLVRRISGHAPGSKEFYRYVLWAQSYQDKETDKMFE
                     KLRDGMG"
     gene            complement(14003..15031)
                     /locus_tag="PLO70_10525"
     CDS             complement(14003..15031)
                     /locus_tag="PLO70_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQZ74946.1"
                     /translation="MKIFYAVQATGNGHISRAMTLLPYLQQYGTVDIFLSGDNSNLQL
                     DAPIKYRSKGISLYYNCKGGLDYWQMIKKIQPWRVKQEIRDLPVEKYDLVLNDFDYIT
                     SAACAKKEIPSIHFGHQASFKSARTPRPGKKSIAGEYLLKHYVRATHYTGLHFDQYDD
                     FIFGPVIKNEILKAEPSAGDHITVYLPSYCEPQLRQLFQAFPDFRFEIFSKETRSIRQ
                     EKNISFIPVNKELFNKSLLSCTGIICGAGFETPAEALQLGKKILAIPIAGQYEQECNA
                     AALEQVGVLCLPAIDKNFATHFTRWIENPSSIQKDYSNSVALSLEHIFSLQTIMQEHE
                     DLLLEETW"
     gene            complement(15045..15887)
                     /locus_tag="PLO70_10530"
     CDS             complement(15045..15887)
                     /locus_tag="PLO70_10530"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQZ74947.1"
                     /translation="MKKRNLDVVVMSDLHLGTYGCHAREIVNYLQSISPRLLILNGDI
                     IDGWQFSKRYFPPSHLQVIKEIMNLLDSDTRVVYITGNHDEMLRRYSGIQLSNFQLVD
                     KLVVEINGKMTWIFHGDVFDTTTKGSAKIIAKLGGFGYDWLILCNRFVNWWLQKLGQP
                     KMSFSKKIKNSVKKAVSWIADFEQTAAELAIEKGYEFVICGHIHQPQMKEIKTKNGTV
                     TYLNSGDWIENLTALEYADNDWTIYHYNEKEFAGANVIPMNKKIPALSVVTDEIGLFI
                     NSLK"
BASE COUNT         4813 a         3338 c         2803 g         5278 t
ORIGIN      
        1 aacaggacgt tagtaaatac gtcggttgcg catttctcat gcagttagcc acccctcgca
       61 gaacaggcag gggtggtatt tttatgtatg gaactgtttt ccatcaagga actgtttcaa
      121 ttaaaaatat tactttaccg gtatatgata tgtgggcttt tagtattcat gcaaaatttc
      181 aaatcccata tgtgcaattt gttagtaatt taaatatcac attcaaattt gaagaggcgg
      241 aatatagtgt acctggatat gtatggtggt aagtatttta gcactcagct tacacaataa
      301 ctttaactta aaagccaaat acaatgaaag gattcttttt agtacatttt ctaatatatc
      361 tatttagctg tgcaacacac tcacagggag gaggaaaaga gtttattgtc aatgaaaaac
      421 tcaaaaataa attttctgat gggccattac caggtgtacg tggcggaccc gatagagttt
      481 atttaaatgg agtattagtt gactcaactt atataaagag tgatattcac tttgtatata
      541 atgcaagtat tcgtgatatt aacggaaatc atttaccctt tgatacttgt acatcttttt
      601 ttgatggcga cacgctaatg tttgtattca aagaaatgaa tgagccatct tcggacaaga
      661 ttgaagtaag gataataaat aaaacatatt caatcatatt tacttcaaat aattcaaatg
      721 aaggaattat tgcatctaac gaatctctaa ttcttaatag cgatctcggt ataaggaaac
      781 gagttgtact tggggatatt gcatttctgg taaatgataa agtgaataat aaacaatact
      841 catttaaggg ctcatttatt tgtaaagttg aataattccc tctcctgtca tttcaaatgt
      901 tcttaaccta atcctcggtt gtgctgaatg tttggttctt tgataatatt aaaatatatg
      961 aaagctgtgc ttcgtacaga cgattaatgt ctgtatttgc aatttgcaac gaagcatcgg
     1021 ctttgcaaaa gccgtaaaaa aaagcagata tacagatttt gatgtttaaa gatttttttc
     1081 tctccagcgt ctccgccgct ccatccgtaa acttttccgc ccatgtctgc cgtgtgtagt
     1141 gttgtgtgcg gcaggcgaca ttgggcacct acggcgcagc atggcacaaa caaaacacta
     1201 attttgttat atgccagtac gtacaaatat gccatataat acaggtcttt attttatcac
     1261 ttttacctgt tacaagtggt tgccactgat agaacaaacc aggagttttg acttagtgta
     1321 taaatggttt gattatctta aaacacaggg gcattctatt acaggttatg ttattatgcc
     1381 taaccatatt catgcactta ttgactttgc agtatctgca aaaaaactaa acactgttat
     1441 tggtgatggt aaacggttca tggcttatga tatagtcaaa agattaaaag aaagagagga
     1501 aacagaaacc cttatgttat tagaaaatgc agtacaatcc aaagacaagg ccaacgggca
     1561 ccgtcatgaa gtgtggaaag agtcatttga ctggaagtat tgcgaaacgg caacgtttgc
     1621 ctatcagaag ttgttgtata ttcataataa cccttgtaac ggcaaatgga acctggcatc
     1681 tgatattaca aagtatgaac acagttcggc gaggtattat atttcgggaa gacatgcggg
     1741 gtatgaagtg aaagatgtgg aggaggtgat aagcaagttg actcagggaa gagcggagtg
     1801 aacaggcgtt aaaagtaaag aaccgcccag cgtccctgct gcgcacaggg ccgggaggag
     1861 gagacgccgg gcagaaaaaa cggtacaaca atgactagta ctcaaaaaga tgtattggct
     1921 ttaggatgta tcggagtcgt aatgttggaa ttgggtatta ccggcaatcc ttcgctctta
     1981 ggaggatata agaattttaa caaagcgcta aaaggtgcta aagagcttca gaaaatgatt
     2041 gacaataatc ctgaggcata tgcggagaat gcaagggtgg ttctatatgg gttttcattt
     2101 caagatgata acgataccta taagtttgac gaggatggaa agctaaattt gactgaagcc
     2161 atgctttatg aaattgatgc agaagctgag aaaaataagc gtggaactaa ctttgatttc
     2221 ggattatata accatagaac tttgacgata tttgatgcag acggtaaaga acctgggatg
     2281 acagtttatg aaagaactat agaaggattt gaagcaattt ttggaaataa gcatgcttac
     2341 tattaccaaa taacaactgt tcctattaaa ttaaaatttt agatatggtg ataagaatat
     2401 gcttgatatc aacaatgttt atttttttta ctgctgtttc aaataaacat gaaggcaatt
     2461 ttaatcaaaa aagaattact gtgattggta aggctgttgt gatgaaacac tatgctgctg
     2521 tgcgcactga cgatagtgtt ttctatttct tagaaggcaa ggatgattgg gacgacaaac
     2581 atctcggaaa gagagtgaag gtatccggga ggttgtatat caaagaatgg catgatgcaa
     2641 agaaaagcga aaatccggaa attactgcaa ttccccaaca acgttatggt gcctggaaaa
     2701 tgttaaaaaa gccaagatgg agtttggttg aatagcggtt agaagatttc attcacttta
     2761 tttgatctaa tacacttgca gaatgtggtc gtggaaataa tttataccca aattcttttt
     2821 ggatcaataa gcatatttct tttatcttga ttatttcagc taacggatcc tctattacgt
     2881 aaacctccca acatgggtat tgaagtaatt tatatttgcc atctttttta acttcaatag
     2941 ttagtgggtc accgtctgca caaactgaat agccggatat ttctttatag tttttaagat
     3001 cataaatacc taacccattt atttttctta tgaaattttt ccaaccagaa ttgggctcgt
     3061 tggtttcctc ttttttactt actgaaacga ttttatacat ttcatcaaat ttcatcttgt
     3121 aataaatggc atgcgaaatc caattattat gttcactttt taatatgata agtttaccgc
     3181 tatcggaaag tgcatactca aaccatattc ttatctctga atcattttgt gtctccttca
     3241 aactaggcag gtcaagcaat ttttcgatct tatttgtggc gttattaaaa gtgtctaatt
     3301 cagtataggt taataccgca ttactatcaa caacaatatc tttactagac agtgtttcct
     3361 ttttttctcg atttcctgta catgacgaaa acgaacaagt tagcattacg gtaaataaag
     3421 aggtaataaa taagtatttc ataataattg tattattcca atttacacgg aattaaatct
     3481 ttgaaagtta aaggtattaa gttcgtcatg gcgcctgggc cataaacgtt ttctgctact
     3541 ctataaattt ttttggcatc ggtgtcagtg ggccattcag ctctgttcgg tgccatcttt
     3601 gctggattgc gtgcccacag aattcccgta gacccaacaa cataaaattt tagatttgga
     3661 aaatcatcca tctttaacat atcgcctttt cctcctccgc tcgacctgct gaatcctata
     3721 ttactgggat caaggtattc acttccttcc caatgcaagt gtatatagcc atacaaagtg
     3781 gcacctttcg gcaatttgcc atgaaatttt ttgggatcag catctggccc gggagaacca
     3841 tatcttttca aaaatgtttt gaatttatcc ggctttgtat acgaataata ccgtacccct
     3901 gtacccttat ctattttttc aaagattaaa gatgcccact cagtttcctt ctcggctgca
     3961 tctctgatac caaggctata tccttccttt ctggcccacg caaatgcagc aatatctgag
     4021 gatgtataat aatcatatcc atcatatgaa aatgtagccg aactaatgct tccataagtt
     4081 ccagctaatt ccccttcatt aaatgaaaag gaaaaatctt tatactccgt tgcacttgcc
     4141 cctgtaattg agataatact tccgccgctt cctgcaaagt ctataatctg atcccataat
     4201 atttctgcat cgcttatttg ccattcttta tccgctttcc attgttgtcg gaatttctta
     4261 ctatccatcc cactcatctc cccgccaaaa aaattatcct cattcaccat tatcgccctc
     4321 attccatccg ggtactacgc tggcatgcct gctggcaggc aggtgttttt gttggcaagg
     4381 gagggagacc gagacctttt aacattacca tatgcgtcgg gaaatatcat caaacctcaa
     4441 accaacactc cgtttaacgg cagtatcatt aattctatcc agtattttgt ttagcaattt
     4501 tttagtatgt tttttattat ctgttatata cctagtcttt tgagattgtg tacaaataac
     4561 atagttataa taacccactt gatttgtatc ctttaatatt ccaacactat caaacccttt
     4621 aatttcattt tttattagct tcaagcattc tcgattaacc ttataagcat agtatggcca
     4681 tgactcattt ttattaaatg tgtcagcata aaaaattatt ttcggaatag gtttatctgt
     4741 taaccagcca tactcaagag taatcgaatt actagtttca catattatgc tatataagaa
     4801 aataaataaa attgataaaa aaccatttgt gatatttttt aagaacttaa ccatatattt
     4861 tatgaatttc ccgctgttgg gtcttcaaaa ccgttgaacg gcctacggcc tttgttggtg
     4921 ttgtgtgctg cctttgtgta attgcaaaaa aaacaacaaa cgaattaaga aatctaagtt
     4981 aggattttcc tacattttaa agccgatgct tcattgcaaa cgaagcacaa ctaccctttt
     5041 taattcttcc ttaccgaaac gaggactcag gagaatggag ataaaaaggg gtgccttcaa
     5101 aagccaatga aatttttgtt aaaggtccga taccacgaaa gttcattatt gtccgctatc
     5161 atccggccaa caacttttct gccttcaata tcaaaaatga ttttacaaaa aaagtttacg
     5221 ttcatttgcc ctccggttac atacagatat atgtattttt tatttggaga taaaaatgct
     5281 tctatgggtc tcttgtttgg ggaaatctcg caaaaatttg gaaaatataa atcactgaat
     5341 atcgaatccg ggataatgaa ccttttatca ttgattgaaa tcgttaaact gtctaatacg
     5401 gagacagttg gtgtatccca tgtaagccca aaaatttctt ttgaatttat tgataaaata
     5461 taagtgtagc catccatgct atgaacagta ttaaaaacgg tagaatcata ttgaatttta
     5521 ccaaggctac ttaagctttt acttattttt tttgcttcta tcctaaactt attggtatct
     5581 attgcgatct gaagagcagc tttgtcatga agcaagaaat catagttgaa tctctcaaga
     5641 aaaaatcgat ttgaaataaa atattttaaa tttcctggcc cggaatattt ccttattaaa
     5701 tgatcaataa agaaactatc gattttatat tctgattgct taggcgacac aatcgtatgt
     5761 cgaaggaggg tttcataagg tttttcaata ttggctcgta tcagccaata ctccttatta
     5821 aaatcatcct gagaaatgca cctttggcct aaaaagaata ttaaaaacaa gaataaaact
     5881 tttttcatct actttaatta atttttttta ttgaaaatgg tccatcggtt ttgaaaagag
     5941 ttactttgta gtaaaaaatt ctttcagcct gtccaaatac ttgcggaaca tttcctcttt
     6001 caagattttt caaaataata tgctctaaaa aaggattacc cattggattt aaaaatggta
     6061 accgctgtaa cctctcatat tgatagagag gaattgcggt tctcaaccaa acatgaccca
     6121 attcatgtac caatcccatt aatgcattct gatagcctac tttttgtgtt attccggata
     6181 caggatcggt atatgataaa atttcaagcc cttcccgatt atcgaaccat atagaaccat
     6241 cttcctttgt ttcagtattt tccattccag cataaattgt aattggtaca tttgaaaatt
     6301 tacctccctc tagaaaatca cataaaacat taccgctcac atttttaacg gtattatctc
     6361 caatagtact gaattgtaaa atccccccat ttttatatag ctcttctaat acggcaaccg
     6421 tttctgaaat agcagtgctt ttgccactgt aggcctttcc tggagtccac acaaacggtg
     6481 tttcattact tccaaaccca tagatactaa tccctccgcc tttgatattc gatccgctac
     6541 ctccacttcc taatttttca cttatggaag ccatatatgc tctgataaat gcagctgtca
     6601 gaatttgagt tagttgctca tcccaatcaa aaaactgctc atgtgaactc caatcttgtc
     6661 cacttctctc aaaccctgtc aactgctgaa accctccctg ctcctcattc atcgcaatgg
     6721 cccgcattcc atccgggtct ataaaccgta ccgggttatt atacgcatag ttatatggtg
     6781 tccatcttct gctaacttcc gctaatgagt ctatcacatg ccagcggcct atctgtgcat
     6841 catattgcct ggcaccatag tcataccact ctaatccatt gccatcagca aattctgctg
     6901 cctgctgttc tttaccgtta tacttgtact tatttactgc acctccaaaa gctaatgctt
     6961 tagaacttat accagcctgt gttaatccaa acgggtaata atggtcttag ttcagtaatg
     7021 ctccccttac atgcgtcacc tgcaaattat caaagaacac atctatattc ggcgtttcat
     7081 tgcttacata aatgtacaaa taaccgctct tgtttatcgc cacatcgtta aacaaatggg
     7141 tggtaaacgt atcactggcg cccacctgct caaaaccact gcctgcactt acatatttaa
     7201 actgctcgtc aaacagtatc cagttgataa acgctttggg ccggctgcta ttgtaagttt
     7261 gattgcttgt gttaatagaa agaaacatac gcaaaaagcg taacacaata caaaagaact
     7321 atgctttaac ttggatgggt ttttaatagc taagtcagta taccaagacc ttattaccag
     7381 agttctttac aaattgaatt aatctcactc tccacctttt cccttaaaat caaatttgtc
     7441 attgttttcg taatctcaaa taaaatccgt tttgtagttt ctttgtcttt tgtaacgtat
     7501 cgcacatctg cgctactatc tttaacggta aaattgtaat atcctagtcc ctgctctggt
     7561 tttatcaata aaacactatc tgatgatact acctttacta ttgcttcgaa ttcaggtcta
     7621 tttattttaa aagccttaaa ataaggggat gtcagctttt gaaatgaatc tacataaaaa
     7681 actaatgtgg taagcggccg attttgcggt ccagcatact caaggataat agtcccccca
     7741 taattattat agttttcaaa accaatgagg gtatagaatt gaactaatgc aataatgagt
     7801 agtttataca taatatactt taaatttgtt tttaaactct gccattaacg gcgcattatt
     7861 tgggcataat ttatcaaatt ttgctgaaac cctgaaggtg atcctatggt tatatttttg
     7921 atactcatat agcttccatt tgcatctttt gtgactacaa gcccatagtt aagagtaact
     7981 aaaggcttgt aacttccttt cttctcctcc catgccatta gagtcaattc gaaatccata
     8041 gttttcgtca tccctattac tggctctctc cctggtgtgt cgaaaaactc tgtgtcgaac
     8101 ccattagcga tattaacgcc atcttttatt tcttgatctg aatgataaaa tggatagcta
     8161 cttcttggta taaatggaac agcatcacat ggatccacaa attgattaac attacaagca
     8221 ccaggatcat tagtcgtaat tgaatgtatc cattcaaaat cacgatatct cgctgaccta
     8281 tttctaaata gcaaattgat catcaacagt tctgcaaatg tccttgttcc atctttaaac
     8341 aatatttctg aaggatttgc ccggaatcta tttacgtaaa ttgtacccac tagcaatcct
     8401 ccttccataa cattgatagt atgtccttct ttatattcaa ttcctgaatt tatacttata
     8461 cctgtaattc cattaacatg cccatctacc actgcctgaa aatcataacc accacttcca
     8521 tttgcattat attgctctag cctatcgaaa atatcattcc aatatgcttc aattgcttta
     8581 ccgattaacg agcttctttg cagtttgttt tgtgaattcc aatcatgccc atgccgacta
     8641 aaccctgtca actgctgaaa ccctccctgc tcctcattca tcgcaatggc ccgcattcca
     8701 tccgggtcta taaaccgtac cgggttatta tacgcatagt tatacggcgt ccatctccgg
     8761 ctcgtttcag ccagcgggtc tggcacatgc catcttgcta tctgtgcatc atactgcctt
     8821 gctccatagt cataccactc caggccatta ccatcagcaa actctgcgga ctgttgctcc
     8881 tttccattgt atttatactt gttagttgca cctccaaaag ccagtgcctt gctgctgata
     8941 ccagcctgtg ttaatccaaa cggatagtaa tgatcttcat tcagtaatgc tccattgaat
     9001 ttggctcttc attgcaaaca aaacacaacc ttcctatttt ttaatattta tcaatgaacc
     9061 aaacactgac gacaaccgga gttaaaaata taagtcatga aattaataag tttcaagtct
     9121 aatcccatcc aacttgttta attatctctt ttaacatctt gtcctccctg agaagcgaat
     9181 cttgttttat ataggcgatg acggtaccag aattgagaaa ctctctcttt ccaggatagc
     9241 ctaccaagtt ttgatactca ttgattatta tcttagctgc tccttccttc atgtcaataa
     9301 gattatagcc gattttacag caggtaactt gatatctgta aagttttgcc acaatactaa
     9361 taatgaaata tttgtattgc ttttgacgcc tcagacatgt tgtatcaatt gtatccataa
     9421 gctgtttcaa tctgggattt aaggattcat ctaaatcaat agctctcgga tcaaagtctt
     9481 taacgtattc taaaagagca gtgtcaatat ccgggcaagc cagtatgtta tctttcttat
     9541 ctgcagattg gtcaggcact ttgttttgag atcctgtaca ttcaaaaagc agaacagata
     9601 aaatagaaaa tgcaataagt ttcattaatt tttttttgat tatcttaata tattaatcgg
     9661 gttaactgtc ttaccgacat cagtttgcct aatatcgata gcgtacccac ctcttcccca
     9721 tccagggatg aacctataaa ctctttccat ttttatgaaa tttcctccgg gggaagtaat
     9781 accacctgtt ccttttccgt taataatttc ctcttgtatt tggtcatttg cagcataagc
     9841 cgttacccca attgccctta ctatcgattc tcctattgaa atatctgtgc caataggaaa
     9901 atctgcaggg cttgcacaat tacatcctcc aaaaataatt gccgcacctt cgtcaaactt
     9961 aatttcaccc ctttctatca ttaattgtaa atctacaacg tccgctgcat ctcccccatt
    10021 gtaagcattt ttgtgattct cagaataaaa gccgttactc tcactaagta taagcccttg
    10081 tccagatgca tgtgaaaaaa agacaattcc agcaatagaa ccatacttcg atgttgccac
    10141 ttttagcgcc ttcaacaact cttttccact ctttacatat attgaagggg cgaaaccctt
    10201 gctttttgcg agtctatctg ctctatcttt aaaaacatcg ttttcacttc ctacatagtt
    10261 tggcgaatta ccctgtccgg caattgacaa ataaattccc cttgattgag ttgactcgta
    10321 tccactgtat ccgtttgaga atgaccaaga agtgtgtccc tcaaaatcaa tttgactatc
    10381 aatactccct ccagcactgg caaacatttc tttcaaatta tcgattagca tttccaatgc
    10441 ctgatcctgt tccatttttt gaaagtatga atcatacctt ttgctccaat ctccctttat
    10501 tctatcgaat cctgttaaac gctgaaaccc tccctgctcc tcattcatcg caatggcctt
    10561 catcccatcc gggtctataa acctaatcgg gttattatac gcatagttat atggtgtcca
    10621 tcttctgcta acttccgcta atgggtctat cacatgccag tggcctatca gtgcatcata
    10681 ctgcctggca ccatagtcat accactccag cccattgcca tcagcaaact ctgcggcctg
    10741 ttgctcctta ccgttatatt tatatttatt cgctgcacca caaaaagcca gtgccttgga
    10801 acttatacct gcctgtgtta atccaaacgg atagtaatga tcttcattca gtaatgctcc
    10861 attgaatttg tcacttcatt gcaaacaaag cacaaccttt catcatttta ttttttatcg
    10921 aacactactg acatcgggga gcgagattct aaaaactttt attaatacat cctattatcc
    10981 atgtttcaag ctgaggagaa ttttcataca ttttgccttg aaacttagta acacctgact
    11041 gtgctttctc aaaagaaatt gatgttttcg ttccatttct atactcgata tcacacaata
    11101 aataaatgct tctcataaca tggcctgcct taattttttt taacctcact atattttttt
    11161 gaatttcaga taagcacaaa ctatcagaaa tttcaaattt tgaagtaata ctaatatttc
    11221 taatattact atccgtaatt ccaatcggtc cacctctatc ttttggcaaa taataaacga
    11281 ctattttcga tatgctcttc tcatgtgaag ctattccaat ttgtccatta gcagatttga
    11341 aaccacagta taaaaaacaa ataaataata aaaatttcac acccatttat ttaatattta
    11401 atatggaata ttgagtttgg aactactgtt aatcctcctc tatgattagt tctaataggt
    11461 tcacctaatc taatagcagc tggagtttca acatatttga ttactcgcat ttcttcaaaa
    11521 tcgtcatagt caccaatttc agggccccat gtaggtaagg tttttctctg atttgtacta
    11581 ttaatatctc tcatatatgc attcgcatga ccaatttcat gtaataacaa caaagccgga
    11641 gattgaattc cgtttaccag gttattttta attacataag atcctgcaaa tggattccaa
    11701 agaatcttat gatgtccgct tctgttaaat aaattatata caggcaagaa ttcagggcct
    11761 aatccaccct taactgaagc cgactcggca tcctcaataa gaataccttc tttacttgcc
    11821 ataagaaata aaacttcaat tccagcacct ccgctaatta aatagttaaa tgcggaaagc
    11881 aactctttaa cataattatt tttctcggca tcatattgta ttccagtaac tgaatcaaaa
    11941 aatccaaaac ttccttccgt attttggcca aaaactaatg tttgattaat atttgatttt
    12001 gaaccatctg aatttgttgt tgacccagag acatttacca atattccagc agaaccgcct
    12061 acaactccaa taaatatatc agttccttca gaagtaccct caccccctaa tttgttcata
    12121 agatcaccta aaacaccatc ccaataatga gacattgcat caccaccaag tttcctattt
    12181 tcctttttac ggctaaaccc tgtcaactgc tgaaaccctc cctgttcttc attcatcgca
    12241 atggccttca ttccatccgg aggatagcag ttttacatac agttggcttc aggcctcaat
    12301 ttcaatgaac ttgcggctat taaaatatta cttttttggt aaattatagt caaaaaaatt
    12361 atatgaaaaa ttttaaaaaa acaaacggga aattgctttt ttcccattgc tggacaagtt
    12421 tattaatgac agcaaaaagg gcagaaggtt acagccaaat ggtaaaaggt taacaaaagg
    12481 aaccattact aactaccaat gtctaaggaa acacttgcaa actttcagta cccaaaaaca
    12541 atttattctc cggttccgct ttattaaaaa ccttaatatg cgtgaaatga ctgctgaacg
    12601 tcattactgg aaacggtttt ataaggcttt ctgcgattac ctgtatgatg acattgggtg
    12661 ttttgacaat tatgcaggaa gccagattaa aattatcaag gcctttttta attttctgaa
    12721 taaggagctt acaatgggaa ttggtgaatt ccaccggagt ttttatgtac ggcgggagaa
    12781 tattgccata ttcccgctaa tgccagagga actaaatttt cttatcagca ataaacattt
    12841 tgaagagcag ctttcgtttc ggatgaagca ggtaaaagat ttttttgttt ttggatgcac
    12901 ggttgccctc cgggtttccg atctatttgc tttaagaaca tccaatatca ggatagtaaa
    12961 tggtatgtat tatcttacag ttcgctctca aaaaacttct gtacacaccc atattaagct
    13021 tcctgaatat gcggtaaaca tcatcaacag aaatactcaa aattcccgtt tactgcccca
    13081 tttttcctcc tgtaacctta accgaagtat aaaaaaacta cttgaactgg ccgggtttaa
    13141 ttacccggtt caactacttc gtgaaaaaag aggaaaggtt attgagttat gcagttccgg
    13201 gcaaagcggc ataagagcaa ggttttgtga tgtatgtgca agccacacta tgcgccggac
    13261 agctataact accatgttga gccttggtat gcaggagcaa ttagtaagac ggataagcgg
    13321 acatgcacca ggcagtaaag aattttaccg ttatgtccta tgggcgcaaa gctaccagga
    13381 caaggagacg gataagatgt ttgaaaaact gagagatgga atgggttaaa aggtctcggt
    13441 ctccctccct tgccaacaaa aacacctgcc agcgtacagc ccaacactat tgcatagagt
    13501 tgggcttgtt acttcatgga gtcatcccat gcttggcctt ggaattggcc cattcgcacc
    13561 cgctgtcatg gtttttggct gcagtcagcc gccttcatcc actgcttatg aatctgtgtg
    13621 gtcaatttgc ttcgcttcat tcaccacaca tcttccgcag ttcctcctat tattcccaca
    13681 gcaggcgaca atcaaaaaag tatgcagaca cacaaaagcg tagcttatac ttttccgctg
    13741 cctggcggtg ggagcgccac ctgctttttt acgcccagcg tctcccctca caatcgttcc
    13801 tgccattgtc tgccgtgtgt agtgttgtgt gcggcaggcg acattgggct aaaacggaga
    13861 agcaggttac aggcgttaac tttgtacaat gctagtacgt tcaaacaacc tgtataaatc
    13921 agaactcgga attgcccggc gatcttgcta cacagagggc cgggaggagg agacgccggg
    13981 ccggaaaagg aaatagatac cgtcaccagg tttcttccag cagcaggtct tcatgctctt
    14041 gcatgatggt ttgcaaggag aatatatgct ccagggacag tgcaacactg tttgagtaat
    14101 ctttctgaat tgaagatggg ttttcgatcc acctggtgaa atgagttgca aaattcttat
    14161 caatagccgg taaacataaa actccaacct gttcaagagc ggctgcatta cattcctgtt
    14221 catattgacc tgctatgggg atggccagta ttttttttcc cagttgtaat gcctcagcag
    14281 gagtttcaaa accggcacca caaatgatcc cggtgcagct gagcaggctt ttattaaaca
    14341 gctctttatt aacagggata aaactgatat tcttttcctg ccgtatacta cgggtttctt
    14401 ttgaaaaaat ttcaaaacgg aaatccggaa aagcctggaa tagttgtcgt agttggggtt
    14461 cgcaataaga aggaaggtat accgtaatat gatctccggc tgaaggttca gctttcaata
    14521 tttcattttt tattaccggg ccaaaaataa aatcatcata ttgatcaaaa tgtaaacctg
    14581 tgtaatgcgt tgctcttaca taatgcttca acaggtattc accagcgatg ctttttttgc
    14641 ctggcctggg tgtgcgggca gatttaaaac tggcctggtg cccgaaatgt atggagggga
    14701 tttccttttt tgcacaggca gctgatgtga tatagtcaaa atcattcagc accaaatcat
    14761 atttttcaac gggtagatcc ctgatctcct gttttaccct ccatggttga atttttttta
    14821 tcatttgcca ataatccagc ccgcctttac aattataata aaggctgatg cctttgctac
    14881 ggtatttaat gggggcatcc agttgcaggt tactgttatc tccactgaga aatatatcga
    14941 cagtgccata ttgctgcaga taaggtaata atgtcattgc acggctgata tgaccattcc
    15001 cggtggcctg tacggcatag aatattttca tggtgattct tttactattt gagtgagttg
    15061 ataaataacc cgatctcatc tgttaccaca ctcagcgccg gtattttctt gttcatgggt
    15121 atcacattgg ctccggcgaa ttccttttca ttatagtgat aaatagtcca gtcattgtcg
    15181 gcatattcga gtgcggtcag gttttcaatc cagtcgccgc tgttcaggta ggtaacagtg
    15241 ccgttctttg ttttgatctc tttcatttgg ggctgatgga tatggccgca gatcacaaat
    15301 tcatatcctt tttctattgc cagttcagct gctgtttgtt caaagtctgc tatccaggaa
    15361 acagcttttt ttacgctgtt ttttattttt ttgctaaaac tcattttggg ctgtcccagt
    15421 ttctgcagcc accagttgac aaacctgttg catagtatca gccagtcata accaaagccc
    15481 cccagcttgg cgattatttt agcactgcct ttggtggtgg tatcaaatac atcaccatga
    15541 aagatccagg tcattttccc attgatctct accaccagtt tgtccaccag ctgaaaattg
    15601 cttaattgaa taccactgta gcgacggagc atttcgtcat gattgccggt aatatatact
    15661 acccttgtgt cgctgtccag caggttcatt atttctttga tcacctgcag gtgtgagggg
    15721 gggaagtaac gtttgctgaa ttgccatcca tctattatgt ctccgttgag gatcagcagg
    15781 cggggtgaga tgctttgcag gtagttgaca atctccctgg catgacagcc ataagtgcca
    15841 agatgcaggt cggacataac aactacgtca agatttcttt ttttcaatgt gtacaggggt
    15901 tttacaaaat acgtatgcac ctattacctg aaagtgaata ctgctttaag gaattgtaat
    15961 atgtatgtga tgtaattata tcttcatttc cgggctatgt atcagtttgc tttgtgaaga
    16021 tgggtctttg gcttaagccg ggtctttccc ttaagctttt attattgtat tttagtcttt
    16081 gactgtaaag attcagaaat cagtgcatgg aagctttttt tcatcaaatg aaacaggtat
    16141 gagaatattt ctcttagcag gattaataat tgtaacctcc tgcggtggtt ctgttgtaag
    16201 caaaaaatta gctgttgctg atagccttgt ta
//
LOCUS       DAORTI010000077        16175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3331_length_16175_cov_7.111166, whole genome shotgun sequence.
ACCESSION   DAORTI010000077 DAORTI010000000
VERSION     DAORTI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_16175_cov_7.111166"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..811)
                     /locus_tag="PLO70_10535"
     CDS             complement(11..811)
                     /locus_tag="PLO70_10535"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQZ74948.1"
                     /translation="MSTNGVKKAGARAKAIQKILITQPRPESEKSPYFELARKYSVEL
                     DFHPFIRLEGIPAKDFRKQKIDIQNYTGIIFTSRNAIDHFFRMCEEMKVIVSQDTKYF
                     CITEAVALYLQKFILYRKRKVFYGADGTNKSMFEVINKHKANEKFMYVCSENQQDNEI
                     VNWLKTNNCEFVLAFMYRSVSSDVKEILMKTEYDVICFFTPSGVKSLFDNLPKFKQNG
                     TVIGAFGNNTFRAIEEKGLKLEIKAPQPQTPSMVAALDQFLANAFKKK"
     gene            complement(841..1824)
                     /locus_tag="PLO70_10540"
     CDS             complement(841..1824)
                     /locus_tag="PLO70_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HQZ74949.1"
                     /translation="MKRFIYLFILLLLLAEISSAQILVDSANSIVIDTTARPDTIVHR
                     DSLPVAQTNLEVSKRPQQDSGWILEPADPVFDQAIMWQILDHHPYFGFKNRVSTVSVE
                     EENIRQVRGKEVLFYSLVLLIIIFALLRQAFPKYFNDLFRLFFRTTLKQRQISEQLIQ
                     TPLPSILLNGFFVISGGFYLSFILEYLKLNPVDHFWLLFIYCMAGLSVAYFVKFVGLK
                     ISGWLFNTEEAANSYIFIVFIINKMIGILLLPFLVLLAFSTENLFSVGLTLSWCLIGA
                     MLIYRVILTYSVVRNQVKVSPFHFFLYFLAFEIAPLLLVYKALLVYFGQTA"
     gene            complement(1880..3685)
                     /locus_tag="PLO70_10545"
     CDS             complement(1880..3685)
                     /locus_tag="PLO70_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQZ74950.1"
                     /translation="MRKFRLQISVLVLLLMSIYTSAQGKYNLHIRGAEKDTALLISRL
                     GLQTSFTSQISCSEYISKLTGILQSKGYATASIDSIRYDSSDAYMVLFAGERYLWARI
                     DAKNIDPSILDGVGWREKVFMNKPMDFAQLQAWQEKLLKYLENNGYPFSKIYLDSLQL
                     ENEMVSAQIKADKGPLYKIDSIRIFGDAKIANSYLQRYLDIPNGSIYNKEKLLRINQK
                     MRELTYVEEERPFDITMLGTGSVLNMYLKQKKSSQINALVGFLPNNDQLASKKILITG
                     EANILLRNSLGAGETIGLNWQQLQVKSPRLNLLYQHPYLFQSPYGLDFSFEMFRKDSN
                     FVNVNFQLGAQYVVNKSQAGKLFIQRFQTIVSQGGVDSAFIVNNRKLPDVGDVSSFNV
                     GVDYELNTTDYRLNPRKGNEFRIITSVGTKKLRKNNEILELKDPSDPSFDFDRLYDTV
                     KLKTYQLRVKLVAAKYFPFANKRSTVKAAINAGMFQSGNIFRNELFQLGGYRLLRGFD
                     EESQYLSQFAIATMEYRYLVGQNSFFYVLADGGWGRNNSLNTNVNYTYFGTGLGLAFE
                     TKAGIFNLAWAVGKRNDTELNLRQSKIHFGFVNYF"
     gene            3752..4861
                     /gene="hemW"
                     /locus_tag="PLO70_10550"
     CDS             3752..4861
                     /gene="hemW"
                     /locus_tag="PLO70_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQZ74951.1"
                     /translation="MAGIYIHIPFCRQACHYCNFHFTTSLRYKNELFTSLLKEIGLQQ
                     DYLQPEPVETIYFGGGTPSLGTIDEIQSIILKTQSCFIVSQNAEITLEANPDDITEDK
                     LAGWKEAGINRLSIGIQSFFEEDLLWMNRAHNAKQAIDNLELAKKYFDNITIDLIYGT
                     PALTNERWKKNIETAISLKIPHLSCYALTVEPKTPLDKLIKHQKSAGVNPDKQSEQFL
                     LLMQWLEEAGYEHYEISNFAKPGWRSRHNSSYWSGKKYLGIGPSAHSFNKTTRQWNIA
                     NNNIYIESINKGEIPFEKEVLTREQQLNEYIMIALRTSEGIDLGSLTNDEVANILKKS
                     KRYIDTLLLKQGNNHLVLTREGKLLADGIAADLFC"
     gene            complement(4858..5064)
                     /locus_tag="PLO70_10555"
     CDS             complement(4858..5064)
                     /locus_tag="PLO70_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74952.1"
                     /translation="MVNFFQVLFLIAAIILLVVNVQAFNLWQESRKESATKPYPIKWF
                     NFILTSLVALGCLLNIIVAWIKKG"
     gene            complement(5243..6280)
                     /locus_tag="PLO70_10560"
     CDS             complement(5243..6280)
                     /locus_tag="PLO70_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQZ74953.1"
                     /translation="MTFGIIGSGSWATALAKMLTDSKHPINWWIRNADTISYIKKRSH
                     NPQYLSSAKFDVSLLTMSTDVKEVVKQSDVLVIAVPSAYAEGSLSGLQPEDWKNKKIV
                     SAIKGLLPDKNMLLNEYLKLEFNIPLENYFAVLGPCHAEEVAEEKLSYLTFSGIDIVT
                     ASEIASHFTTDFISTVVNHDILGVQYAAVLKNIYALGAGIAHGLDYGDNFLSVYIANA
                     ADEMAGFLRKFGAEHITVGEHDGEDPVTHRKTPNYAASVYLGDLLVTCYSLHSRNRRF
                     GNMIGRGYSVKAAELEMNMVAEGYNAAKCIFIINKSLDAGIPIAETIYKILWEDVKAS
                     EGFKRIEQVLV"
     gene            complement(6410..7756)
                     /locus_tag="PLO70_10565"
     CDS             complement(6410..7756)
                     /locus_tag="PLO70_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ74954.1"
                     /translation="MNKKLKWGLIVVAVLGAGFFLVKKFGSKDKTEKVAIEKAIKRTI
                     IETVNASGKVYPEVEVKISPDISGEITELNVEEGDSVRKGQVLARIYADIYALQRDEA
                     ASRVNQSSATVDNSKAALEALKANLELAQQNYNRNKKLFDDKVISKSELEQYETTLKS
                     AKSNLNAAEQNIKGLRASVQGTQVGLSKANKDLSRATLTAPMDGVISSLKIKKGERVA
                     GNSFNVGTEMMTVADMSVLEVRVDVGENDIVKINIGDSADVEVDAYNNRKFKGIVTKI
                     ASSTKSTSLASTNDVTNYEVRIRLDKESYKDIGNRTFPFRPGMNASADIKTRRVDNVL
                     AVPISAVNARVKGSDQSMADKKKEKEDSKGEEGNDVSATVEGDELEEVAFVLQKDGTV
                     KKIVVKSGIQDINYIEILSGLKEGDEVVVAPYAAVSKNLKDGMKVKVVTKDKLFEK"
     gene            complement(7789..9237)
                     /locus_tag="PLO70_10570"
     CDS             complement(7789..9237)
                     /locus_tag="PLO70_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ74955.1"
                     /translation="MVRSIKLAFALLIFSTSFGQDKWDIRRCVDHALKNNISVRQTDL
                     QSRFSELSYKQNKGGQLPSLNFGSSVGYRLGRSDNPTTGVLEDNNFLNVGMQLQSSVS
                     LFNWFSQKNTIEASRLSWEADKEQTKKIQNDIALNVAVAYLQILLAREQANLSNVQVE
                     QTKAQLENTRKRVDAGVLPELNAAELEAQLARDSSSLVTAEASVQQFLLQMKALLNLD
                     AGASFDIVTPPVSLIPLEALADLQPDAVYGIAISTLPQQKVNELRIQSAKKTVAAAKA
                     GLYPTISAFGSLSTNAISFKKAIYDQVLNGYSPSGARANAGGGTFYPIEIPNYVDGSN
                     VVGYYKPGSITRQFNSNFGQSIGIGLNVPIFNGRIARTAWDRSKLTVQQFELQKEQGD
                     MQLKQDIYKAYTDATAALQKFNADKKSVQAAEKAYDFASKRYELNLLSTYDLIISQNN
                     VQRTRIQALYSQYDYVFKMKLLEFYKGQGIKL"
     gene            complement(9295..>9543)
                     /locus_tag="PLO70_10575"
     CDS             complement(9295..>9543)
                     /locus_tag="PLO70_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQZ74956.1"
                     /translation="LFTFALKTDKRESIVRFCESLQHIMMAVSWGGHESLVIPKCAGI
                     QPGDFDGSNREHRYIRMYVGLEESKYLIADLEQALAGI"
     assembly_gap    9544..9643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9644..10254)
                     /locus_tag="PLO70_10580"
     CDS             complement(<9644..10254)
                     /locus_tag="PLO70_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQZ74957.1"
                     /translation="MDISQIINELGEDREYYFNAVAPPIMQTSNFAFNKVADLSKAFE
                     NEMGGYLYSRGLNPTVDILRKKLAALDGAEDCLVFNNGAAAIFAGIFANLKYGDHIVS
                     VKAPYTWVQRMFDVILPRFGVTATYINGGKIENWEKATQPNTTFYYLESPNSWDFAIQ
                     PIKEVAALAKSRGIITLIDNSYCTPLYQKPVEMGIDIAMQTAT"
     gene            complement(10255..11589)
                     /locus_tag="PLO70_10585"
     CDS             complement(10255..11589)
                     /locus_tag="PLO70_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQZ74958.1"
                     /translation="MTAKNKLGLFSLTMIVIGLVIGTGIFRTSKDAAEAATTPTIFFA
                     AWIVAGLVAICGALTYAEIGSRYPVTGGYYKIFSYAYHPSLAFGINAIILISNAASLS
                     GVALIGSEYITPLLIEAGSVEKWKPFVAMAAIILFYGVNLMGLRMSSQTQNVLMLIKI
                     GMLLVLIAALFFPAIHSTAPVIIDESKTSAAALLLSFGVALKATSFTYGGYQQTINFG
                     EEVENPQKNIPKGIFIGIVVIICLYLLVSYAYVKVIGFNNLKETSGIASVVAEKLFGE
                     AGKYGSSILLFLAVLAYVNVLLLSNPRVMYAMSTDGILPKIFQKKDEKRDVLTVSLTV
                     FAAICIVVLFFADKFEKILSFTIFLDSIGMATSAATIFILRRKTRHLDGTGIYAMKLY
                     PLLPVLFILAYTFVGTMIAVKEPELALTGISVLAAFILLFFIVKQLQKDKTQ"
     gene            11645..13720
                     /locus_tag="PLO70_10590"
     CDS             11645..13720
                     /locus_tag="PLO70_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQZ74959.1"
                     /translation="MRRFFIFLVFIYSLLPAFGQEETTDSSTLLEEVTIRAFEQNRHA
                     SLTTATVKIIDRNATEMNNKSSFVNAFNTIAGVRMEERSPGSYRINIRGSSLRSPFGV
                     RNVKVYWNDIPLTDPGGNTYFNQLAQNNFSIIEIFKGPAGSMYGAGTGGLILINNLDR
                     WQPGASLEYITGSYKLQNVFASARFGQKENKNQFTFAHNQSDGYRVQSKMRRDNFSWI
                     SQLKLSAKQQLTASVLFSDMYYQTPGALTLAEYNTDPASARPAAGGFPSAVNVNAAIW
                     QKNITAGFTNHYQFNTHFTNTTTFYGTFNQVKNSAIRNFERRNEPHIGGRSVFNYTIK
                     NEETTWQLTAGTEYQSGFFNTQVAKNRNGNPDTLQTNDDIRYTTYTLFLQADVLINNT
                     WSINGGLSTNKTKVNFIRLSTYPVTEQKRSYQNEIAPRISLKKDFRRELTLKGTISKG
                     FSPPTISELLPSTGVISTNLEAENGWNYEITVSKPFLRKKLQLQLTGFYFRLNNALVQ
                     RRDISGADYFINAGDIKQKGLEVHSSYLLLAKNKSLVDYLNLRADYTYSHFRYGSFIQ
                     GIDDYSGKIVPSVPANTLSFTGDLQLKNGIYTNITFYAASGIFLNDANSATAEPYQLM
                     GARIGWSKRLQNKYKLNLYAGADNLLDEIYSLGNDINAAANRFYNAAPRRNYYAGVSF
                     QWIKALKKK"
     regulatory      13907..14122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14224..16164
                     /locus_tag="PLO70_10595"
     CDS             14224..16164
                     /locus_tag="PLO70_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ74960.1"
                     /translation="MKRHFFVLAAVIFSNQLKAQQDTTLLNEVVITTNKYPKKQSETG
                     KVVTTITREQLERSGGKTLPEILNSATGTVIIGANNALGTNQTVSIRGASAGNVLILL
                     DGIPVNDPSVITNYFDLNFLTTDQVERIEILKGGQSTLYGSDAVAGVINIISKKAGAK
                     KLHVHGAFSGGSFNNLRQNIGINGRGNKADYSLNYTHLSNDGFSAAFDSSGSAGFDRD
                     GFNQHAITGRTGFAAGKTIRISLSGSYSNYQTDIDASAYTDDRDFTSESNNIQFGGGL
                     LHHFSNGSIHLNYNYNRIERSYLDDSSHKSNLFAFYSKSNYIGRTHYAELYGNWRMSQ
                     WEILAGTDFRSNNTDQWYWSTGIFGPFNQPVLNARAKQISPYASVIYKEKGLNVELGG
                     RFNEHSAYGSNFTFTFNPSFLVKNKAKVFANLYSAFKAPTLYQLFDAYAGNASLLPEK
                     GIIGEAGAELLSGKPISARIVGFYRKTTDAIVYTFNPTVFQSKYLNASKQINNGIEAE
                     LGYAKNIFSISANYTYTNGKTTSPYDGTGSPLGKDTSYYNLYRIPKHAFNLTAGIRFS
                     KDFFVSTHFRSLSKREEFIYGSSPETIDGYAIVDLYGEYKLGKAIRIFVDLKNITNKV
                     YFDIPGYNARKFNFITGLSFQL"
BASE COUNT         4928 a         3489 c         2918 g         4740 t
ORIGIN      
        1 ttttgtagaa ttatttcttc ttaaaagcat ttgcaagaaa ttgatccaat gcagccacca
       61 tactcggtgt ttgtggttgc ggagctttta tttccagctt caatcccttt tcttctattg
      121 ccctgaaagt gttatttcca aaggctccga tcacagttcc gttttgttta aatttcggca
      181 gattatcaaa gaggctcttt actccgcttg gggtaaagaa gcagataaca tcatattccg
      241 ttttcataag aatttctttg acatcactgc ttacactgcg gtacataaaa gcaagaacga
      301 actcgcagtt gttagtcttc aaccagttta caatttcatt atcctgctgg ttttctgaac
      361 atacatacat gaatttctca ttagccttat gcttgttgat aacttcaaac atacttttat
      421 tagtgccatc agcaccataa aacacttttc tcttcctgta aagaataaat ttctgaagat
      481 aaagggctac ggcttcagta atgcagaagt acttggtatc ctgtgaaacg ataaccttca
      541 tttcttcgca catacgaaaa aaatggtcaa ttgcatttct gctggtaaaa ataataccag
      601 tatagttttg aatatcaatc ttttgctttc ggaaatcctt tgccggaata ccttccagtc
      661 ggataaacgg atgaaaatcc aattctacac tgtatttcct ggcaagctcg aaatatggcg
      721 atttctcgct ttcgggcctt ggctgggtaa tcaggatttt ctgtattgct ttggcccgtg
      781 caccggcctt tttaacccca tttgtactca tgttcggttc ggctaaagtt ggctgcaaag
      841 ttaagcagtt tgaccgaaat acaccaacaa agccttgtaa accaataaaa ggggtgcaat
      901 ttcaaaggct aaaaaatata aaaagaagtg aaatggactg actttgacct gattacgtac
      961 tactgagtag gttaaaatga ccctgtaaat cagcatagcc ccaataaggc accacgaaag
     1021 agtaagccct accgagaata aattctccgt tgaaaaggcc agcaaaacaa gaaatggcag
     1081 caacaaaatg ccaatcattt tattaatgat aaacacaatg aaaatgtagg aattagcagc
     1141 ttcttccgtg ttgaataacc agccagatat tttcaaaccc acaaacttga caaaatatgc
     1201 cactgaaagg cctgccatgc agtaaataaa taaaagccag aaatgatcaa ccggattgag
     1261 ttttaaatac tccaatataa agctgagata aaacccaccg cttattacaa aaaatccatt
     1321 caataatata gaaggtagtg gcgtttgtat caactgctca cttatctgtc tttgcttaag
     1381 ggtagttctg aaaaaaagtc tgaacaagtc attaaaatat ttagggaaag cctggcggag
     1441 taatgcaaaa ataataatga gtaagaccag gctataaaaa agtacttctt ttccccttac
     1501 ctgcctgata ttttcctctt caacagaaac agtagaaacc ctgtttttaa aaccaaagta
     1561 tggatgatga tcaagtattt gccacataat ggcctggtca aaaaccggat cagcaggttc
     1621 taatatccag ccggaatcct gctgcggacg ttttgaaacc tccaaatttg tttgtgctac
     1681 aggtaacgaa tcacggtgca ctatcgtatc cggcctggct gtagtatcaa ttacaattga
     1741 attggctgaa tcaaccagaa tttgagcaga ggaaatttcc gccagtaata acaagagtat
     1801 gaaaagatat ataaaccgct tcaagtaaga atgttgtgat gcaaaagtat taaagtggag
     1861 tccgactcta tgcaacgatt taaaaataat tcacaaaacc aaagtgaatc tttgattgac
     1921 gaaggttcaa ttcggtatca tttcttttcc caactgccca ggccagattg aaaataccgg
     1981 cttttgtttc aaatgcaaga ccaaggcctg ttccaaagta ggtataatta acattggtgt
     2041 taaggctgtt gtttcttccc catcccccat ccgccagcac atagaaaaaa gaattttgcc
     2101 caaccaggta acggtattcc atggttgcaa tagcaaattg cgataaatac tgactttctt
     2161 catcaaaacc tctgagtaat ctatatccgc ccagctggaa aagttcatta cgaaaaatat
     2221 taccactttg aaacattccg gcattgatgg ctgcctttac agtactccgc ttattggcaa
     2281 agggaaaata ttttgcagcc accagcttta cccgaagctg gtaagttttc aatttaaccg
     2341 tatcataaag cctgtcaaaa tcaaacgatg gatcagaggg gtcttttaat tccagtatct
     2401 cattattttt tctcaatttt tttgttccca ccgatgtgat aatacgaaac tcatttcctt
     2461 ttcggggatt tagccggtag tcagtagtat tcaattcata atccacacct acattgaaag
     2521 aacttacatc tcctacatcg ggcaacttcc ggttattcac aatgaatgca gaatcgactc
     2581 cgccctggct aactatagtc tgaaaccgct gaataaatag cttgcctgcc tggcttttat
     2641 ttacaacata ttgtgccccc agttgaaaat taacgttgac aaaattgctg tccttacgaa
     2701 acatttcaaa tgaaaaatca agcccataag gcgattggaa aagataaggg tgctggtaca
     2761 agaggttcag ccggggcgac ttcacctgca gctgttgcca gttcaaacca attgtttcac
     2821 cagctcctaa tgaatttctc aacaaaatat tcgcctcgcc ggttattaat atttttttag
     2881 atgccagctg atcattgtta ggtaaaaatc caaccagggc atttatctgg ctgcttttct
     2941 tctgctttag atacatgttt aatacagatc ctgtacccag catagtaata tcaaagggcc
     3001 tttcttcttc tacatacgtg agctcccgca ttttctgatt gatacgcaac aatttttctt
     3061 tattgtatat actaccattg ggaatatcaa gatatcgctg gagataactg ttagcgattt
     3121 tggcatcacc aaaaatacga atgctgtcaa tcttgtataa tggccctttg tctgctttta
     3181 tttgagccga taccatttca ttctccagtt gcaggctatc aagataaatt ttcgaaaagg
     3241 ggtaaccgtt attctccagg tacttcaaca gcttttcctg ccaggcctga agctgtgcaa
     3301 aatccattgg cttattcata aatactttct cccgccatcc tacaccatct aaaatagaag
     3361 gatcgatgtt tttcgcatct atccgggccc acagatatct ttctcccgca aataaaacca
     3421 tataagcatc cgaggagtca tacctgatgc tgtcgatgga tgcagtagca tagcccttag
     3481 attgcaaaat accagttaac ttgctgatat attctgaaca ggaaatctgg gatgtaaaac
     3541 tagtttgcag gcctagccgg gagatcagta atgcagtatc tttttcagcc ccacggatat
     3601 gtaaattata tttaccctgc gctgatgtat aaatgctcat caacaacaga acaagaaccg
     3661 atatttgcag tctgaatttt ctcatgtggt ttttcacctg tcccgatagc cggcgggatg
     3721 tcgctaaatt attcattaaa tattaaacac tttggctggc atctatatac atattccgtt
     3781 ttgcaggcag gcctgtcatt attgcaattt ccattttaca acatccttac ggtacaaaaa
     3841 cgaattattt acatctttat tgaaggaaat tgggttacaa caagattacc ttcagccaga
     3901 acctgttgaa accatctatt tcggcggagg cacaccaagt ttaggaacaa ttgatgaaat
     3961 acaaagtatc attttaaaga cacaatcctg ttttatcgtt tctcaaaatg ctgaaataac
     4021 ccttgaagca aatcctgatg atattacgga agataaactg gctggctgga aagaagcagg
     4081 catcaatagg ttaagcatcg gtattcaaag tttttttgaa gaagaccttt tgtggatgaa
     4141 ccgggctcac aatgctaaac aggccattga taacttagaa ttggccaaaa aatattttga
     4201 caacatcact attgatctga tctatggtac gccagcctta acaaacgaaa ggtggaaaaa
     4261 aaatatcgaa acagccattt cgctcaaaat tccccaccta tcctgctacg ccttaacagt
     4321 ggaaccaaaa acaccccttg acaaattgat aaagcaccaa aaatcagcag gtgtaaatcc
     4381 cgataaacaa tcagaacaat ttttgttact gatgcaatgg ctggaagaag ccggctatga
     4441 acactatgaa atatccaatt ttgccaaacc aggctggcga agccgtcata actcatcgta
     4501 ttggagtggt aaaaaatatc tcggcatcgg tccttcagcc cattctttta acaaaacaac
     4561 caggcaatgg aatattgcca ataataatat ttacatagaa tccattaata aaggagaaat
     4621 cccttttgaa aaggaagtgc tgacaagaga acaacaacta aacgaatata tcatgattgc
     4681 gctgcgtacc agtgaaggca tcgacctggg aagtttaaca aatgatgaag tggcaaatat
     4741 tttgaagaaa agtaaaagat atatcgatac ccttttactg aagcagggaa ataatcatct
     4801 ggtcctaaca agagaaggca aactgctggc ggatgggatt gcagccgatc tgttctgtta
     4861 accttttttg atccaggcta cgataatatt cagcaaacag ccaagggcta ccagggacgt
     4921 tagaataaaa ttgaaccatt tgatggggta tggtttagtt gcactttctt tccggctttc
     4981 ctgccataaa ttaaaggcct gaacattaac aacaagtaaa ataatagcag cgattaaaaa
     5041 caaaacctgg aaaaaattta ccatgaacta attattttaa cagctgtgaa gttaaaattt
     5101 tcaaagcaat aaatttattc cttataatac tgacggcgga aatatttata gatctgactg
     5161 actaccaggc aagctatcat tatcaatata gcagcaggta caaatccgtg ataaaacagt
     5221 ggcatagggc aacatttact ggtcagacca gcacctgttc tatccgttta aagccctctg
     5281 aagctttaac atcttcccac aatattttat aaattgtctc cgcaatagga ataccagcat
     5341 caagtgattt attgataata aaaatacatt ttgctgcatt atacccttcc gccaccatgt
     5401 tcatttccaa ctcggcagcc ttcacagaat aaccccggcc gatcatattt ccaaatcgac
     5461 gattacggct atggagtgaa taacaggtta ccagtaagtc acccaggtac acagaagcag
     5521 catagttggg tgttttcctg tgggtgacgg gatcttcgcc atcatgttca cctacggtaa
     5581 tatgctccgc accgaatttt cttaaaaacc ccgccatttc atcggcggca ttggcaatgt
     5641 aaacactcag gaaattatcc ccgtagtcaa gcccatgggc aataccggca cccagtgcat
     5701 atatattttt taaaactgca gcatattgca cgcctaaaat atcatgattt acaactgtac
     5761 tgatgaaatc agtagtaaaa tgagaagcaa tttctgaggc ggtcactata tcaattcctg
     5821 agaaagtgag ataggacaat ttttcttctg ccacctcttc tgcatggcag ggaccgagta
     5881 cggcaaagta attttcaagc ggtatattga attccagctt taaatactca ttaagaagca
     5941 tattcttatc aggcaataat ccttttattg ctgaaacaat ttttttattc ttccagtctt
     6001 cgggttgcag accagaaagg cttccttctg cataggcgga cggaacagct ataaccaaca
     6061 catctgactg cttcactact tcctttacat ctgttgacat tgtaagcaat gacacatcaa
     6121 acttggctga acttaaatat tgaggattgt ggcttctctt tttgatgtag ctgattgtat
     6181 ctgcatttct gatccaccag ttaatggggt gcttgctatc tgtcagcatt ttggccaatg
     6241 ctgtagccca actgccacta ccgataattc caaaagtcat gaaccctgat ttcgttgaaa
     6301 aaataattgt aaacaataaa ggtaataaag gaataaaata ctccgtaaaa aagaaaaccc
     6361 ggaataaacc gggttttacc actaattcat tttattgttt ccgatacagt tatttttcaa
     6421 acaatttatc cttcgtcact acttttacct tcataccatc tttcagattt ttgctaactg
     6481 ctgcatatgg cgcaaccacc acttcgtctc cttcttttaa tccactgagt atttcaatat
     6541 aattgatatc ctgtattccg gattttacta ctattttttt aacagttcca tctttttgca
     6601 atacaaaagc cacttcttcc agttcatccc cttcaacagt agctgaaaca tcattccctt
     6661 cttccccttt gctatcctct ttttccttct ttttatctgc catactctgg tcacttcctt
     6721 ttacccttgc atttacagct gaaatgggca cggctaatac attatccacc cttcttgttt
     6781 taatgtccgc actggcattc atacccggac gaaaaggaaa agtacggtta ccaatatctt
     6841 tatatgactc cttatctaat cttatcctca cttcgtaatt cgtaacatca ttggtggaag
     6901 ccagtgatgt actttttgta ctgcttgcta tttttgtaac aatcccttta aacttgcgat
     6961 tattatatgc gtccacttcc acatctgctg aatcgccaat attgattttt acaatatcgt
     7021 tttctcccac atccactcta acttccagta cagacatatc tgccactgtc atcatttctg
     7081 tacctacgtt aaaactatta ccggccactc tttctccttt ctttattttc aatgacgata
     7141 tcactccatc catcggagcc gtaagtgtgg ctcggcttaa atccttgttt gccttgctta
     7201 aaccaacctg ggttccctgt acactggctc gaagcccttt tatattttgt tcagcagcat
     7261 taagattaga ctttgccgac ttcaaggtag tttcatactg ttccagttcg gatttggaaa
     7321 ttactttgtc atcaaacaat tttttgttcc ggttatagtt ctgttgtgcc agttcaagat
     7381 tagcttttaa agcctctaaa gctgctttgc tgttatcgac agtagcagaa gactggttta
     7441 cccttgatgc agcttcgtct ctttgcaatg cataaatatc agcataaata cgggcaagca
     7501 cctgtccttt tcttacacta tcgccttctt ctacattcaa ttcggtaatc tcgccggaaa
     7561 tatccgggct tatttttact tccacttccg ggtaaacctt tccgctggca ttgaccgttt
     7621 cgataattgt acgtttgatc gctttttcaa tggcgacctt ctccgtttta tccttactcc
     7681 cgaatttctt tacaagaaag aatccagccc ccaacactgc tactacaatc aatccccatt
     7741 tcaatttctt attcatcgta tatttttatt aaaatttgat ctgaaagttt acaattttat
     7801 tccctggcct ttataaaact cgagcaattt cattttgaat acataatcat actgtgaata
     7861 caatgcctgt atcctcgtcc gctgcacatt attctggcta ataatgagat catacgtaga
     7921 caacaggttt aattcatacc ttttactggc aaaatcataa gctttctctg ctgcctgcac
     7981 actcttctta tctgcgttga atttctgcaa tgctgctgtt gcatcggtat atgctttgta
     8041 gatatcctgt tttaactgca tatcaccctg ttctttctgt aattcaaact gctgcaccgt
     8101 aagttttgaa cgatcccatg ctgtccgggc aatcctgcca ttgaaaatgg ggacattaag
     8161 acctatacca atgctttgac cgaaattact attaaattgc ctggtgatgc ttcccggttt
     8221 ataataacca acaacattac tgccatctac ataattagga atttcaatag ggtaaaaagt
     8281 gccgccaccg gcattggctc ttgcgccaga tggggaatag ccatttaaca cctggtcgta
     8341 aattgctttt ttgaatgaaa tagcattggt actaagactt ccaaatgcac taatcgtagg
     8401 atataaacca gcttttgctg ccgccaccgt ttttttggca gactgaattc ttaactcgtt
     8461 aactttttgc tgtggcaatg tacttattgc aatgccatag actgcgtcag gctgtaaatc
     8521 agccagtgct tcaagcggga tcagactaac cggaggtgtc actatatcaa atgaagcacc
     8581 cgcatcaaga ttcagcaatg ctttcatttg cagtaaaaac tgttgtacag atgcttctgc
     8641 tgttacaaga cttgaactgt ctcttgccaa ttgcgcttcc agctcagctg catttaactc
     8701 aggtaacact cccgcatcta ccctctttct tgtattttct aattgtgctt ttgtttgctc
     8761 cacctgcaca tttgataagt ttgcctgctc tcttgccagt aatatctgta agtaagcaac
     8821 ggctacattt aaagcaatat cgttctgaat tttttttgtc tgctctttat cagcttccca
     8881 tgaaagacgg ctggcttcta tagtgttttt ctgtgaaaac cagttgaaaa gactgacact
     8941 ggattgtaat tgcataccca cattcaggaa attattgtct tccagaacgc cggtagttgg
     9001 gttatctgac cggccaagcc ggtagccaac gctgcttcca aaatttaaag aaggcaactg
     9061 cccgcccttg ttttgtttgt agcttaattc agaaaaccgc gactgcaaat ctgtttgccg
     9121 tactgaaata ttatttttca acgcatgatc aacacatctt ctgatgtccc atttatcctg
     9181 gccaaatgaa gtagaaaaga tcaacaatgc aaatgccagc ttgatgctcc gtaccataga
     9241 ctgcaaaaat atatgaaaag ccgccgcagt gttatgattt ttgacaagcg gggattaaat
     9301 tcccgcaaga gcttgttcaa ggtctgctat caggtatttc gattcctcta atcccacata
     9361 catacggata taccgatgtt cccggttgga tccgtcaaaa tcgcccggct gaataccggc
     9421 acacttagga atgaccaggc tttcatgccc gccccagcta acggccatca taatatgttg
     9481 caaagactcg cagaacctta caatggactc tcttttatct gtttttaatg caaaagtgaa
     9541 taannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttggtag cggtttgcat
     9661 cgcaatatca attcccattt cgacaggttt ttgatacagc ggcgtacaat aactattatc
     9721 tattagtgta ataattcccc ttgactttgc cagtgctgct acttctttga ttggctgaat
     9781 ggcgaaatcc cagctattag gagattccag gtaataaaat gtggtattgg gctgagtcgc
     9841 tttttcccag ttttcaattt ttcctccatt gatataagta gcagtaactc caaagcgggg
     9901 taatatcaca tcaaacattc tttgcaccca tgtataagga gctttgacag atacaatatg
     9961 atcaccgtat tttaaattag caaaaatacc tgcgaaaata gcggctgccc cattattaaa
    10021 taccaggcaa tcttctgcac catctaatgc agccagtttc ttcctgagaa tatcgaccgt
    10081 tggatttaac ccacgactgt aaaggtaacc gcccatctca ttttcaaacg ctttactcaa
    10141 atcagccact ttattaaaag caaaattgct tgtctgcatg attggtggtg ccacagcatt
    10201 gaaatagtac tcccggtctt cacccagctc attgataatt tgtgaaatat ccatttattg
    10261 agttttgtcc ttttgtaatt gcttcacaat aaaaaacaat agtataaatg cagctaaaac
    10321 acttatacct gtcaatgcca gttcaggttc ttttacggcg atcatcgttc ctacaaatgt
    10381 ataggcaagt ataaacagta ccggaagaag tggatataat ttcatagcat agatgcctgt
    10441 tccatcgaga tgccgggttt ttctccgcaa aataaaaatt gttgctgctg aggtagccat
    10501 accaatcgag tccaaaaaga tagtgaaact taaaatcttc tcgaatttgt ctgcaaagaa
    10561 taaaacgaca atacaaattg ccgcaaacac cgtcaggctg accgtaagca catccctttt
    10621 ctcatctttt ttctgaaata tctttggcaa aataccatca gtactcatgg catacattac
    10681 ccgcggatta ctaagcagta atacatttac ataagccagt actgcgagga ataataaaat
    10741 agaagatccg tacttacctg cctctccaaa caatttctca gccaccaccg aagctatgcc
    10801 acttgtctcc tttaaattat tgaaaccaat tactttcaca taagcgtaac tgaccagcag
    10861 gtacaaacaa ataatcacga cgattccgat aaaaataccc tttgggatat ttttttgagg
    10921 attttcgact tcctccccaa aattgatagt ttgctggtac cccccatatg taaatgaggt
    10981 agcttttaaa gccacaccaa atgaaagcaa taatgcggcg gctgaagttt ttgattcatc
    11041 aataataacg ggggccgtac tatgaatagc cggaaagaat aatgctgcga ttaatacaag
    11101 caacataccg atcttaataa gcatcagcac attctgtgtt tgcgaactca tccttaatcc
    11161 catcaggtta acgccataaa aaagtatgat cgctgccatt gccacaaatg gcttccattt
    11221 ttccacactg ccagcctcta tcaaaagcgg agtaatgtat tcactgccta ttaatgccac
    11281 ccctgataaa gaagcggcat tggaaataag tataatcgca ttaattccaa atgccagtga
    11341 agggtgatac gcataagaaa atatcttata gtaaccaccg gtaaccggat accggcttcc
    11401 tatttcagca tatgttaagg ctccgcaaat agctaccagc cctgcaacta tccaggctgc
    11461 aaaaaaaata gttggagttg tggcagcctc tgccgcatct ttagatgtac ggaaaattcc
    11521 agtgccgata acgaggccga tcactatcat tgtcagtgaa aacagtccga gtttattctt
    11581 agcagtcatg gattgaagat acaaagaaaa caatctaact ttatcctctt acaattcagt
    11641 aaccttgagg agatttttca tttttttagt attcatctat tctttattgc ccgctttcgg
    11701 ccaggaagaa acaacagaca gctcaactct tttagaggaa gtaacgatca gggcttttga
    11761 acaaaaccgg catgcatcgc ttactacagc aaccgtaaag atcattgatc gaaatgctac
    11821 tgaaatgaat aataaatcat ctttcgttaa tgcattcaat actattgccg gagtacgaat
    11881 ggaagaaagg tccccgggca gctatcgtat caatatccgg ggcagttcgc ttcgttcgcc
    11941 ttttggcgta agaaatgtaa aagtttactg gaatgatatt ccccttacag accccggtgg
    12001 taatacctat ttcaaccaac tggcacaaaa caatttttct atcatagaaa tttttaaggg
    12061 gcctgccggt agtatgtacg gcgccggcac aggtggattg attttgataa acaatctcga
    12121 caggtggcaa ccgggtgcca gcctggaata tataaccggc agctataaac tccagaatgt
    12181 ttttgcctct gcccgtttcg gacaaaaaga aaataaaaac caattcactt ttgctcataa
    12241 ccagtccgat ggttaccggg tacaatcgaa aatgagacgg gacaatttct cgtggatatc
    12301 acaactgaaa ttatcggcta aacaacaact aacagcatcc gtcctttttt cggatatgta
    12361 ttatcaaaca cccggggcat tgacattggc agagtataat actgatcctg catcggcaag
    12421 gccggcagca ggtggatttc cttccgcagt aaatgtaaat gcagcaatct ggcagaaaaa
    12481 cattacagca gggtttacca atcactacca gtttaatacc catttcacca atacaaccac
    12541 cttttatggc acttttaacc aggtgaagaa ctctgcaata cggaattttg agaggcgaaa
    12601 tgaacctcat attggcggcc gctctgtttt taattatacc ataaaaaatg aagaaactac
    12661 ctggcaattg acagccggta cagaatacca gtcagggttt ttcaatacac aggttgctaa
    12721 aaacaggaat ggcaatcctg atacattgca aacaaatgat gatatcaggt acacaacata
    12781 caccctcttt ctgcaggctg atgtccttat taataatacc tggtcaataa atggcggtct
    12841 aagcactaat aaaacaaaag taaatttcat ccggctaagt acatatcccg tcacagaaca
    12901 aaagagaagt tatcagaatg aaatagcccc acgaatttcc ctgaaaaaag atttccggcg
    12961 ggagttgaca ttaaaaggga ctatatctaa aggcttctca cctcctacga tctccgagtt
    13021 acttccctct acaggagtga tcagtacgaa cctggaagca gaaaatggct ggaattatga
    13081 aataactgtt tctaagccct tcctccgcaa aaaacttcaa ctgcaactga ccggttttta
    13141 ttttcgcctg aacaatgcac ttgtacagag aagagatatc tccggcgcag attattttat
    13201 caatgccgga gacataaaac aaaaaggtct tgaagtgcat agcagttacc tgctcctggc
    13261 aaaaaataaa agcctggtgg attacctgaa cctgagggca gattatacat acagtcattt
    13321 ccgttatggc agttttatcc agggcattga tgactactca ggcaaaatcg ttccttctgt
    13381 tccggccaat acactctcat ttactggtga tctgcaattg aaaaacggca tctatacaaa
    13441 tatcactttt tatgcagcat ctggaatatt cttaaatgat gccaattcag ctacagcaga
    13501 accttaccaa ctgatgggtg ccaggatagg ctggagcaaa cggttacaga acaaatacaa
    13561 gctgaatctt tatgccggag cagataattt actggatgaa atttatagcc ttgggaatga
    13621 cattaatgca gcagctaacc gtttttacaa tgcagccccc cgccgtaatt attatgccgg
    13681 ggtatctttt caatggatca aagctttgaa aaaaaaataa tgcggttcat atgaaatttc
    13741 agcaatagta aatccaggtt aaataagtaa taaacagagc tgcacattca gcctttaccc
    13801 cataagaaat catagaaaat caatcttcca aactcactga ttcttggtaa tagatatgga
    13861 caagtaagga tgttgataac tcttttcagt acaaaacttc ttcctcctac ttttgtatcg
    13921 atttggttcc cgacctaaca atcgggatta aaagggaaac ccggtgtact tgcctgccga
    13981 aggcaaagag tccggtgcta tccccgtagc tgtaagctcc tttattgatt gtttaatctt
    14041 caagccactg tccggttggc ggacgggaag gcattaaaca acggagtaag ccagaagacc
    14101 tgccacgtcg agcaaacaac attcagcttt cgggtgaaag gcatgagatg taaaagtcgc
    14161 aggacgttta tatttctatc acttttttat ttccggaagc agtcacacaa aatttcattt
    14221 tatatgaaaa gacatttttt tgtgctggct gctgttattt tcagcaatca gctaaaagca
    14281 caacaggaca caacattgct aaacgaagtg gtaattacca ccaacaagta tccaaaaaaa
    14341 caaagtgaaa cagggaaggt tgtcaccact attacccggg aacaattaga aaggagtggc
    14401 ggcaaaacac ttcctgaaat tttgaatagt gcaacaggaa ctgttattat tggcgccaat
    14461 aatgccctgg gcaccaatca aacggtcagt atccgcggtg cttcggccgg aaacgtattg
    14521 atattgctgg atggcattcc ggtaaatgac ccttctgtaa taacaaatta ttttgattta
    14581 aactttctaa ccactgacca ggtagaaaga attgaaatat taaaaggtgg gcaatctact
    14641 ttgtatggat cagatgctgt ggccggtgtt attaatatca tttcaaaaaa ggcgggtgca
    14701 aaaaagctac atgtacatgg cgctttttcc ggtggaagct ttaacaactt gcggcaaaat
    14761 ataggtatta atggccgggg aaataaagca gattattcgc tgaattatac acatctcagc
    14821 aacgatgggt tctcagctgc cttcgactcg agcggatctg cgggttttga cagggatggt
    14881 ttcaatcagc atgccattac cgggcggaca ggttttgctg ccggcaaaac catcaggatc
    14941 tctttatcag gaagctatag caattaccaa actgatattg atgcctctgc ttatacagac
    15001 gacagggatt ttacttcaga aagtaacaat atacaatttg gcggtggcct gctgcatcat
    15061 tttagtaacg gaagtattca tcttaattac aactacaaca ggattgaaag aagttatctt
    15121 gacgattctt cacataaaag caatttgttt gctttttact caaaaagcaa ttatatcgga
    15181 cggactcatt atgcagaatt atatggcaat tggagaatga gtcaatggga aatacttgcc
    15241 ggaacagatt ttcgctccaa taatacagac caatggtatt ggtctactgg tatatttggg
    15301 ccatttaatc aaccggtact taatgcccgg gctaaacaga tcagtccata cgcatcggtt
    15361 atttataagg aaaaagggtt aaatgttgaa ttaggcggac gcttcaatga gcattcagcg
    15421 tatggaagta atttcacttt tacgttcaac ccctccttcc tggtcaagaa caaggcaaag
    15481 gtttttgcca atctttactc tgcttttaaa gcacctacat tataccaatt gtttgatgcc
    15541 tatgctggca atgcatccct tttaccagaa aaaggaatca taggagaagc gggtgctgaa
    15601 ctgttatccg gtaaaccgat ttcggcacgg atcgtaggtt tttaccggaa aacaacagat
    15661 gcaattgtat acacattcaa tcctaccgtt tttcaaagca aatatcttaa tgccagtaaa
    15721 caaatcaaca atggtatcga agcagaacta ggctacgcta aaaatatctt tagcataagt
    15781 gcaaactata cctatactaa tggcaaaacg acttcgcctt atgacggtac aggcagccca
    15841 ttgggcaaag acaccagcta ctataattta taccgtattc ccaaacatgc ctttaacctt
    15901 actgcgggca tccggttcag caaagatttt tttgtaagca ctcatttccg cagtctcagc
    15961 aaacgggaag aattcattta tggttcatca cctgaaacca ttgatggtta tgcgatagtg
    16021 gacctgtatg gtgaatacaa gttaggaaag gcgataagaa ttttcgtaga tctgaagaat
    16081 attactaata aagtatactt cgacatccct ggttataatg caaggaaatt taatttcatc
    16141 acaggcctga gttttcagct gtaaaccagt ttcta
//
LOCUS       DAORTI010000078        16131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3350_length_16131_cov_6.440781, whole genome shotgun sequence.
ACCESSION   DAORTI010000078 DAORTI010000000
VERSION     DAORTI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16131
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_16131_cov_6.440781"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PLO70_10600"
     CDS             complement(<1..152)
                     /locus_tag="PLO70_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQZ74961.1"
                     /translation="MNKQEFTITVYTENQVGLLNRIAIIFSRRKINIESLNTSPTEVD
                     SVHRFT"
     gene            complement(173..1921)
                     /gene="ilvB"
                     /locus_tag="PLO70_10605"
     CDS             complement(173..1921)
                     /gene="ilvB"
                     /locus_tag="PLO70_10605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQZ74962.1"
                     /translation="MDTLELIKEQTKKQVTTTISGGEAVLEACLAEGVETIFGYPGGA
                     IMPIYDALYDYKEKLKHILVRHEQGAIHAAQGFARVSGRTGVVFATSGPGATNLVTGL
                     ADAMIDSTPVVCITGQVFAHLLGTDAFQETDVINITTPVTKWNYQVTDATEIPTVLAK
                     AFYIAGSGRPGPVLIDITKNAQIQKFEYEGYTKCDHIRSYRPKPIVRKEYVEQAAKLI
                     NEAERPFVIFGQGVILGKAEKEFKQFIEKAGIPAAWTIMGMSAIPTGHPLGVGMLGMH
                     GNYGPNVLTNECDVLIAIGMRFDDRVTGRLDKYARQAKVIHLDIDPAEIDKNVKADIP
                     VWGDCKETLPMLTNLVEQKVYPQWLQKFKDYQQKEEEKVIYDELNPTGEVLTMGEVIR
                     VLNELTEGNAVIVTDVGQHQMVACRYAKYNQTKSNITSGGLGTMGFALPAAIGAAYGD
                     PSRTTVAVIGDGGFQMTLQELGTIMQFKPNVKIIILNNQFLGMVRQWQQLFNEKRYSF
                     VDIQSPDFVQVAKGFGIPGKSICKREDLKTSLKEMLESKESFLLEVMVGKENNVFPMV
                     PQGRGVAEIVLSKEEI"
     gene            complement(1932..3617)
                     /gene="ilvD"
                     /locus_tag="PLO70_10610"
     CDS             complement(1932..3617)
                     /gene="ilvD"
                     /locus_tag="PLO70_10610"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQZ74963.1"
                     /translation="MSELNKYSKTLTQDPTQPAAQAMYYAIGFKEEDFNKAQVGIVSM
                     GWDGNPCNMHLNDLAVSVRNSVNSTKGLLGLRFYTIGVSDGISMGTDGMRYSLVSRDV
                     IADSIETNAGAQYYDGLIAIPGCDKNMPGAIMAMGRLNRPSIMLYGGTIAPGHYNGED
                     LNIVSAFEALGQKIAGNLSEGDFKAIVQHSCPGAGACGGMYTANTMAAAIEALGMSLP
                     NSSSNPALSEDKQKECAAVGEAIKLLLQKNITPKDIMTRKAFENAITVVMVLGGSTNA
                     VLHLIAMAKSVDVELTQDDFQTISDRIPVLADFKPSGKYLMEDLHKHGGVPAVMKYLL
                     LNGLLHGDCLTVTGKTVTANLAGVPDLDFMTQNIIHPLELPLKKKGHLQILYGNLAEK
                     GSVAKISGKEGERFEGTARVFDGEKDLIAGIQSGKVHAGDVVVIRYIGPKGAPGMPEM
                     LKPTGAIIGAGLGKSVALITDGRFSGGTHGFVVGHITPEAFDGGLIALVEDNDIIEID
                     AVNNTLNLKVNDEVISARRKNWKQPALKATKGVLYKYAKSVKPANEGCVTDEA"
     gene            complement(3614..4513)
                     /locus_tag="PLO70_10615"
     CDS             complement(3614..4513)
                     /locus_tag="PLO70_10615"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HQZ74964.1"
                     /translation="MTYYNEHTVLYLNGTFVRAAEATMDYYSQSLHYGYSVFEGIRSY
                     KTINGETKIFKAKEHYDRLQQSAEAMNMPYTWSAEELIVATYELLKRNNLQDAYIRPL
                     VYAPANMSFVQNKESFLVIEAWEMQPFLGEKLLRVMLSSFQRPNPKGFRITAKAGGHY
                     VNSILASQEAKANGFDEALLSDMNGFIAEGPGANVFYEKDGKLFTPSVGNILPGITRA
                     TVFEICEEMNIPVEEKLITTEELKEADAVFFCGTAAEVIGWESLDNVKFPKPWNDSVS
                     KTIQNFYKDKVIEKENKPTLELA"
     gene            complement(4798..5913)
                     /gene="leuB"
                     /locus_tag="PLO70_10620"
     CDS             complement(4798..5913)
                     /gene="leuB"
                     /locus_tag="PLO70_10620"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQZ74965.1"
                     /translation="MKKRIAVIEGDGIGPEVTRQAVRILNAVAESFNHEFEYTYCLMG
                     ADAIDKTGTPLPDETIEICLKSDAILFGAIGHPKYDNDPTAKVRPEQGLLKLRKSLQL
                     YANIRPVSTYSSLFHLSPLKSKNIEGVDFIIYRELTGGIYFGKKETSADGNLASDDCS
                     YSREEIERIAHLAFQHAQKRKKKLTLVDKANVLETSRLWRKVVQELASQYTEVAVDYM
                     FVDNASMQIIVNPKQFDVILTENMFGDILSDEASVISGSLGLLPSASIGKTSALFEPI
                     HGSYPQAAGKDIANPIGSILSAAMLLDHLDMKEEAQVVRDAVNWTLLNSFVTKDIDPV
                     NFYFTSTIGELICDFVSGKISGSVNTENIELRKSTII"
     gene            complement(5919..6503)
                     /gene="leuD"
                     /locus_tag="PLO70_10625"
     CDS             complement(5919..6503)
                     /gene="leuD"
                     /locus_tag="PLO70_10625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQZ74966.1"
                     /translation="MKALTTIQSRFVPINMENIDTDQIIPARFLKATTKDGFGKNLFR
                     DWRFINDDETKPKSDFPLNQSQYSGEILVAGKNFGCGSSREHAAWSILDAGFRIVVSS
                     FFADIFKNNALNNGVLPVQVTDAFLQKIFQQDNKAELTVDLEKQTITIHSTGEQEYFD
                     INSYKKTCLLNGYDDIDYLLSIKEDIQEFETQRG"
     gene            complement(6507..7907)
                     /gene="leuC"
                     /locus_tag="PLO70_10630"
     CDS             complement(6507..7907)
                     /gene="leuC"
                     /locus_tag="PLO70_10630"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQZ74967.1"
                     /translation="MGKTLFEKIWEKHIVKTIDGGPSVLYIDKHFIHEVTSPQAFTGL
                     NKRGIKVLRPQQVVATADHNVPTLNQHLPIKEEQGRLQVEALKINCAEHNIQLYGLGH
                     PFQGIVHVIGPELGITQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVFASQCLLQ
                     SKPKLMRINVDGVLNKGVVSKDIVLYILSKITASGATGYAVEFAGSAIRSLSMEARMT
                     ICNMSIEMGGRCGLIAPDDTTFHYIKGRQFAPQGTQWDKKVAEWKQLYSDDEATFDRE
                     IFIKAEDIEPMITYGTNPGMGIGVTEKIPTTETINENDKSGLSKSLAYMGLQQGVAIK
                     GQKIDYVFIGSCTNSRIEDLRMVASFVKGKKKADDVEVWIVPGSKQVEAQAIEEGIDK
                     IFAEAGFQLRQPGCSACLGMNEDKIPAGKYCISTSNRNFEGRQGAGARTLLASPLTAA
                     AAAITGVVTDVRELLN"
     gene            complement(7914..9083)
                     /locus_tag="PLO70_10635"
     CDS             complement(7914..9083)
                     /locus_tag="PLO70_10635"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQZ74968.1"
                     /translation="MAEGKVHIFDTTLRDGEQVPGCKLNTTEKLELALQLEDLGVDII
                     EAGFPVSSPGDFESVSQIAKTIKNATVCGLTRAVQNDIEVAALALKYAVRPRIHTGIG
                     TSDLHIKAKFNSTREEILERAIQCVKWARNFTDDVEFYAEDAGRTDNDYLARVVEAVI
                     KAGATVVNIPDTTGYCLPYQYGEKISYLINNVPNIDRAIISCHCHNDLGLATANSIAG
                     VIAGARQIECTVNGLGERAGNTSLEEVAMIIKQHKDLGLYTTIKSEKLNPISRLVSDT
                     MRMPVQPNKAIVGSNAFSHSSGIHQDGFLKDAQTYEIMNPEEVGADSSKIVLTARSGR
                     SALAYRFQKLGFQFDRNDIDVLYNEFVKVADSKKEVEDGDLASIAKQYQSKVAIA"
     gene            9294..10844
                     /locus_tag="PLO70_10640"
     CDS             9294..10844
                     /locus_tag="PLO70_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74969.1"
                     /translation="MPNRSTDSLFQLVKSLEKSEKRNFKLYVKRNTATDDLKIIQLFD
                     AMDKMTDYDEKQLLKKARGISKQQLSNIKAHLYKQILSSLRLIKDEDNVDIRLHEQMD
                     HARILYNKGLYLQSLKVLERLKETAKAYQQLTYWQQALFFEKKIETLFITRSMQDRAD
                     KLSAESDGVNFQLTLVNQFSNLSLQLYSWYIKHGHARNEKDIKSIETFFRKHLPAETH
                     HAKGFYEKLYLYQCYVWYAFIRQDFLQYYRYSQKWVSLFEEYPGLLKVETASYIKGMH
                     NLMGAQFNLANHEKLAQSIKHFEQFVKQKHLAENENNRILAYQYLYAARINLYFLQGT
                     FTKGLRLVPFLEEMLKEYELYLDSHRVLVFYYKIASLYFGSGNNEKAIDYLNRIINQK
                     GDLRTDLQCYARLLHLIAHYELGNFDLLEYLIKSVYRYMARMENLSKVEEAMFVFLRR
                     SFHVGAMALKPEFEKLLAKLKQYEGNPLEARAFAYLDVISWLESKISGVHVQDVIREK
                     FLLGKRKL"
     gene            complement(10933..12018)
                     /locus_tag="PLO70_10645"
     CDS             complement(10933..12018)
                     /locus_tag="PLO70_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74970.1"
                     /translation="MKRLYFFILLLFPLALSAQKSVDLDKYRFSVQYRSLPKIRIDST
                     YRTYNVEVEGTKLMEPLLQDMSPEKSVMLEGWRKLAEDGHLSIKINLGDLLPEVVSVK
                     ERVVTTKNRDGVITGTKTYYYQEVVYTFSANASITDYKGMHIMDEELASRGHKQVYRS
                     PEFTIRALAEGYFVVNSLTVTKQLLQNCVNRAMHHLSERISDNFGFREVTANDYMWII
                     DSRKHPEYAACRQAFRVMNEVLFSMNPSTSIEGAREQLKPAIDYFEKIKKNYTSSKKH
                     DRKIRYASYFNLAVLYYYLDDPQSMMKEANGLVLNDFDPKDGKGFEQTATWLKNLFQQ
                     TNIYTRHFAINTDELKGPYEKRDISVK"
     gene            12251..13438
                     /locus_tag="PLO70_10650"
     CDS             12251..13438
                     /locus_tag="PLO70_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQZ74971.1"
                     /translation="MNLYTSRSVIIITCPKRLAPYLAQEVLELGFSIDETFVTGVRLS
                     GTINDCIKLNLNLRCASQVLYSLKQFEAENADEIYNHLIAYPWEMILPDPGYFSITSN
                     VNNPTINNSMFANLRVKDAIVDRMREKRGTRPSTGSELTGAVIHLFWKNERAEIFIDT
                     SGDSLGRHGYRKIPGQAPMLEALAAATIYATRWDRKSPFVNPMCGSGTLAIEAAMIAT
                     NRRPGLFRTNYAFMHLQGYDEAVYQLEDALLEKQIIDVPGLRIIATDYSAKAVENAKK
                     NAVAAGVATIIDFAVCDFAATEIPQDVPGVFYVNPEYGERLGDIKELEATYARIGDFM
                     KQKCGGYYGYVFTGNLELAKKIGLKAKRRIEFYTSTIDCRLLEYELYSGSRNVPKEIK
                     ENT"
     gene            13465..13731
                     /locus_tag="PLO70_10655"
     CDS             13465..13731
                     /locus_tag="PLO70_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HQZ74972.1"
                     /translation="MEISALQQIVRERYFKTDNERGIYHTALWFHEEVGELSSAIASG
                     DKQNAKEEFADVLMWLLTLANLMEVDMQEVVADYLVNSRKLPSK"
     gene            13845..14117
                     /locus_tag="PLO70_10660"
     CDS             13845..14117
                     /locus_tag="PLO70_10660"
                     /inference="COORDINATES: protein motif:HMM:NF023174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HQZ74973.1"
                     /translation="MQYMIIEKFHTGKVKAMYQRFEERGRMLPEGVIYINSWINETVT
                     VCYQVMESESPEKIQEWVSQWNDLVDFEVIPVITSAKAKEKVFTEI"
     gene            complement(14131..15456)
                     /locus_tag="PLO70_10665"
     CDS             complement(14131..15456)
                     /locus_tag="PLO70_10665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQZ74974.1"
                     /translation="MSSQKNATDSILLKLKIKSLNEMQHAAIEAMDSHNNTLLLSATG
                     SGKTLAFLLPLIKLLDPANTKTQAIIIVPSRELALQIESVFKSMGTNLKVTCCYGGHL
                     RETEENNLKQPPALLIGTPGRLADHIRRGNITTASITTLILDEFDKSLELGFLDEMSF
                     IIGALPAIQKRILTSATQAVEIPGFIGMNDSVTLNFLTDKDEDTGLTIKTLLSDGKDK
                     LETLFRLLCMLGNKSTIIFCNHRESVERTSKFLKEKDIINEFYHGALEQQERDAALSK
                     FRNGTANVLVTTDLASRGLDIAHIRYIIHYHLPGSEDIFTHRNGRTARMDASGTAILI
                     LSPEEKLPAYINPDTEQIILPESTTLPDKPQWSTLFIAAGKKDKVNKIDIVGFLSNKG
                     KLKKEDIGLIDVKDFFSFVAIKKIKVSQTLQLIKEQKIKNKKVKIAVAK"
     gene            15656..15874
                     /locus_tag="PLO70_10670"
     CDS             15656..15874
                     /locus_tag="PLO70_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74975.1"
                     /translation="MKKIIGFLSLAAVLTTLSCTDKPAEVKKEVIVVPSSPTIIVKDP
                     PAKSTTVTLDKNGVKVESKKVDVTVKKQ"
BASE COUNT         4434 a         3417 c         3187 g         5093 t
ORIGIN      
        1 attgtaaagc gatgtacact atcgacttca gtaggagagg tgttcaggct ttctatattg
       61 attttccttc ttgaaaatat aatggcaata cggttcagca aaccaacctg gttctctgta
      121 tatacggtaa ttgtaaattc ctgcttgttc attttcgtct aattaatgat gatcagattt
      181 cctctttact taatactatt tctgccacac cccgaccctg aggtaccatg ggaaatacat
      241 tgttttcttt tcctaccatt acttccagta gaaaactttc ttttgattcc agcatttctt
      301 ttaaggaggt tttcaagtct tcccgtttgc aaatgctttt acctggtata ccgaagcctt
      361 ttgccacctg tacaaaatca gggctttgaa tatccacgaa tgaatatcgt ttctcattaa
      421 atagttgctg ccactggcga accataccca ggaactggtt attgagaatg atgatcttta
      481 cattcggttt aaactgcatg atagtgccta attcctgcaa agtcatctgg aaaccaccat
      541 caccaatgac ggcaacagtt gttcttgacg gatcgccata tgctgctcca attgcagcgg
      601 gtaaagcaaa tcccatagtg cctaaaccgc cgctggtgat attacttttt gtctgattat
      661 attttgcata ccggcaagct accatctggt gctggcccac atccgtaacg attaccgcat
      721 ttccttctgt cagttcattg agtaccctga ttacttcacc catagtcagg acttcacctg
      781 tcgggtttag ttcatcatat attacttttt cttcttcctt ttgctgatag tctttaaatt
      841 tttgtaacca ttgcggataa actttttgtt cgaccaggtt tgtcagcatg ggtaatgttt
      901 ccttacagtc tccccacaca ggaatatcag cttttacatt tttatcaatc tctgcggggt
      961 caatatcaag gtgaataact ttagcttgtc tggcatattt gtccaaacga ccggttaccc
     1021 tgtcatcgaa acgcatacca atggcgatta agacatcaca ttcattggtt aagacattgg
     1081 ggccataatt gccatgcata cctaacatgc ctacacccag cggatgacct gtaggaatgg
     1141 cactcatacc catgatggtc caggcagcgg ggatgccggc tttctcgata aattgtttga
     1201 attctttttc agcttttcca agaataacac cttgtccgaa gatcacaaat ggcctttcag
     1261 cctcgttgat gagtttggct gcttgctcca catattcttt tctgacaatt ggctttggac
     1321 gataacttct gatatgatca catttagtgt atccttcgta ctcaaatttt tgtatctgtg
     1381 catttttggt gatatctatt aacaccggtc cgggtcttcc gctgcctgcg atatagaacg
     1441 ctttagctaa tactgtaggg atctccgtag catcggttac ctggtaattc catttggtaa
     1501 caggagtagt gatattaatg acatcagttt cctggaaagc atcagtaccc aataaatgtg
     1561 caaatacctg cccggtaata cagacaacag gagtggagtc gatcatggca tcagctaaac
     1621 ctgtgacaag attggtagct cccggaccac tggtggcaaa cacaacaccg gttcttccgc
     1681 tcacccttgc aaagccctgg gcggcatgaa tagctccctg ctcatgacgc accaatatgt
     1741 gtttcaactt ttctttataa tcatacaatg catcatagat gggcatgatg gcgccgccgg
     1801 gataaccaaa tatggtttca acaccttcag ccaggcatgc ttccagcacc gcttcgcctc
     1861 cgctgattgt cgtcgtaacc tgtttctttg tttgctcctt tattaattcc aatgtatcca
     1921 taacaattat tttaagcttc gtcggtaaca cagccttcgt tagccggctt aacagacttt
     1981 gcatatttat acaagactcc ttttgttgct ttcaaggcag gttgtttcca gttttttctt
     2041 cttgcggaaa tcacttcatc atttactttt aaattcaaag tgttatttac agcatcaatt
     2101 tcaataatgt cattgtcttc caccagggcg atcagtccgc catcaaatgc ttcaggtgtg
     2161 atatgtccca ccacaaaccc atgtgtccct ccgctgaagc ggccatcggt tatcagggct
     2221 actgattttc ctaaacctgc tccaatgatg gcaccagttg gtttcagcat ctccggcata
     2281 cctggtgccc cttttggtcc aatgtaccgg atcaccacca cgtcaccggc atgcactttt
     2341 cctgattgaa tacctgctat cagatctttt tctccatcga acactcttgc tgttccctca
     2401 aaacgttccc cttctttacc gctgatcttt gcgacacttc ctttctcagc taaatttcca
     2461 tacagaattt gcaggtgccc cttttttttc aatggtagtt ctaacgggtg aataatgttc
     2521 tgtgtcataa aatcaaggtc aggaacacct gctaaattgg ctgttaccgt tttaccggtt
     2581 actgtcaaac aatcgccatg gagtaagcca tttaataata aatatttcat tacagcggga
     2641 acaccgccat gtttatgcaa atcttccatc aggtattttc ctgatggttt gaaatccgcc
     2701 agaacaggta tacggtcact tattgtctga aaatcatctt gtgttaattc gacatctaca
     2761 ctttttgcca ttgcgatcaa gtgcaaaact gcattggtac tgccacctaa taccatcaca
     2821 acagtaattg cattttcaaa tgctttgcgg gtcatgatgt ctttgggagt aatatttttt
     2881 tgcagtaaca attttattgc ttcgccaacg gcagcacatt ctttttgttt gtcttcactt
     2941 aaagccggat tggaagagga gttgggtaaa ctcataccca atgcttcaat tgcagcggcc
     3001 attgtattag ctgtgtacat accaccacaa gcaccggcgc cgggacatga atgctgaacg
     3061 atcgctttga aatcaccttc actcaggtta ccggcgatct tttgtcccaa cgcttcaaat
     3121 gcacttacaa tatttaaatc ttcaccgtta taatgacccg gagcaatggt accgccgtat
     3181 aacataattg acggacggtt caatcttccc atggccatga tagcaccagg catattttta
     3241 tcacagcctg gaatggcgat caggccgtca taatactggg cgcctgcatt cgtttcaata
     3301 ctatcagcga tcacatcacg gcttaccaaa ctataacgca taccatccgt tcccatactt
     3361 atcccgtcac ttacaccaat agtatagaat cgtaaaccta ataacccttt agttgaatta
     3421 acactgttac ggacactaac agccaggtca ttcaggtgca tattgcatgg gttgccatcc
     3481 catcccatac tcacaatgcc tacctgtgct ttattgaaat cttcttcttt aaaaccaata
     3541 gcatagtaca tggcctgggc agcaggttgt gtcgggtctt gtgtaagtgt cttactatat
     3601 ttatttaatt cactcatgcc agttctaatg ttggtttatt ttctttctca attactttgt
     3661 ctttatagaa attctgtatt gttttactta cagaatcatt ccatggtttt ggaaatttta
     3721 cattatccag gctttcccag ccaattactt ctgcagcagt accacaaaaa aatacggcgt
     3781 cagcttcttt caattcctct gtagtgatta gcttctcttc aactggtata ttcatctctt
     3841 cacaaatttc aaaaacggtt gccctggtaa taccgggcag gatattccca actgacggag
     3901 taaataactt tccatctttt tcataaaaca catttgcccc gggtccttct gctataaagc
     3961 cattcatgtc ggataataat gcttcatcaa atccattggc ctttgcttcc tggctggcca
     4021 aaatggaatt aacataatgt cccccggctt tagctgttat cctgaatcct ttagggttag
     4081 gacgctgaaa ggaacttaac atcacccgta ataatttttc acccaaaaaa ggttgcattt
     4141 cccaggcttc tattacaaga aacgattctt tattttgaac gaaactcata ttagccggtg
     4201 catacacaag tggtcttata taagcatcct gcaggttatt tctctttaat agctcatagg
     4261 tcgcaacaat caattcttct gcactccatg tgtaaggcat attcatcgct tcagcagact
     4321 gttgaagacg atcatagtgt tcttttgcct taaaaatttt agtttctccg ttgatcgttt
     4381 tatacgaacg aattccctcg aacacggaat atccataatg taaactctgg ctatagtaat
     4441 ccattgtagc ttcggccgct cttacaaagg tgccattcag atataaaaca gtatgctcat
     4501 tatagtaggt catatatttt catttaggtc ataaaaaaaa cccgcctttt tgaaggcggg
     4561 tttgatattt ttagtttatt aacttaatgt catgcaccac ctccatttga agaagatata
     4621 ataatcacca ccataataat aattgcagca acagaggtta tgacattata tgtgttattc
     4681 ttcaacatga aggatgtatg tgttgtaaat atacacccgc cgtttcaaaa aaagaactca
     4741 gcaagcaata attatttcag taatggacaa accttcgtta gttatttacc cattgattta
     4801 tataattgtt gattttctca actcaatatt ttcagtattt acagaaccgg atattttccc
     4861 actcacaaaa tcacagatca gttcaccaat cgtggaagtg aagtaaaaat ttaccggatc
     4921 aatatctttt gtaacgaacg aattcagtaa agtccagttt acggcatccc ggaccacttg
     4981 tgcttcttct ttcatatcca ggtggtcgag cagcatagct gctgaaagaa tagaaccaat
     5041 ggggtttgca atgtccttgc cggcagcctg cgggtaagaa ccatgaatag gttcaaacag
     5101 ggccgaagtt ttgcctatag aagctgaagg taataacccc agcgagccac ttatcacact
     5161 ggcttcatca cttaaaatat cgccaaacat attttccgta aggataacat caaattgttt
     5221 tggattcaca atgatctgca tggaggcatt atccacaaac atgtaatcga cggccacctc
     5281 agtgtattgt gaagccagtt cctgtacaac ttttctccac agcctggatg tttcgagtac
     5341 attggcttta tcaaccagtg tcagtttctt ttttcttttc tgcgcatgct gaaaagcaag
     5401 atgggcaatt cgttctattt cttcccggct ataactgcaa tcatctgatg ccaggttacc
     5461 atcagcactg gtttcttttt taccaaaata aattccaccg gtcagttccc tgtaaataat
     5521 aaagtctaca ccctcaatat tcttactttt taagggggag agatgaaaga gggaagagta
     5581 agtactaacc gggcggatat ttgcatacag ctgtaatgat tttcgcaact tcaggaggcc
     5641 ttgttcaggt cttactttag ccgttggatc attatcatat ttcggatgcc cgatagcgcc
     5701 aaataaaata gcatcactct tcaggcaaat ttcaatggtt tcatctggaa gcggggtgcc
     5761 tgttttatca attgcatcag cacccatcag gcaataagtg tattcaaact catggttaaa
     5821 tgattctgct acggcattca gtatacggac tgcctgcctg gttacttccg ggccaatacc
     5881 atcaccttca attacggcta ttcttttttt catagtcttt atcctctttg ggtttcgaat
     5941 tcttgtatat cttctttgat actgagtaaa taatcgatgt catcataacc gttcagcaaa
     6001 caggtttttt tataactgtt gatatcaaaa tactcctgtt cccctgttga gtgtatagtg
     6061 atggtttgtt tttccaggtc aacagtcaat tcagctttat tatcctgctg aaaaattttt
     6121 tgaaggaaag cgtcagtaac ctgtactggc agcacaccgt tattcaaggc attgttttta
     6181 aaaatgtctg caaagaaact ggacaccact atacggaaac ctgcatctaa aatagaccag
     6241 gctgcatgtt cccttgatga gccgcaaccg aaatttttac cggccactaa tatctcacca
     6301 ctgtattgtg attgatttaa gggaaaatcg gatttgggtt ttgtctcatc atcatttatg
     6361 aatctccagt cacggaacaa gtttttgcca aaaccatctt tagtggttgc cttcaggaaa
     6421 cgggccggaa tgatctggtc tgtatcgata ttctccatat tgatcgggac aaacctgctt
     6481 tgtatcgttg ttagtgcttt catcgtttag ttcaatagct ctcttacatc tgttacaacc
     6541 cctgtaatag ctgctgcagc ggctgtcaac ggactggcca ataaagttct ggcaccggca
     6601 ccttgtctgc cttcaaaatt tctattagaa gtgctgatgc aatatttccc ggccgggatc
     6661 ttgtcttcat tcattcccag gcatgcgcta caacctggct ggcggagttg aaatcctgct
     6721 tctgcaaaaa ttttatcaat accttcttct attgcctgtg cttctacctg tttacttccg
     6781 ggtactatcc atacttccac atcatcagct ttctttttac cttttacaaa ggaagccacc
     6841 attctcaggt cttcaatcct tgagttggtg caactcccga tgaatacata atcaattttt
     6901 tgtcctttga tagcaacacc ttgttgtaaa cccatgtagg ctaatgattt tgataaaccg
     6961 gatttatcat tttcattaat tgtttcagtg gttggaattt tttctgttac accgattccc
     7021 attcccgggt tagtgccata ggtgatcatc ggctcaatat cttctgcctt gataaaaatc
     7081 tctctatcga atgtagcctc atcatctgaa taaagttgtt tccattccgc tactttttta
     7141 tcccattgag taccctgtgg ggcaaattgc cttcctttta tatagtggaa ggttgtatcg
     7201 tctggtgcga tcaacccgca acgaccaccc atttcaatac tcatgttgca gattgtcatc
     7261 cttgcttcca tacttaagga acggatggca gatccggcaa attctactgc atagcctgtg
     7321 gctccgctgg ccgttatttt tgacagtata tacaggacaa tgtcttttga cacaacacct
     7381 ttatttaaaa caccatccac attaatgcgc atcagtttgg gtttactttg caataaacat
     7441 tgcgatgcaa aaaccatttc cacttcactg gtgccaatac caaatgcaat agcaccaaat
     7501 gcgccgtgtg tggaggtatg gctgtctccg caaacgatcg tcataccggg ttgtgtaatt
     7561 cccaactcag gaccaattac atgcacaata ccctggaagg gatgtccaag gccatataat
     7621 tgtatgttat gttctgcaca atttattttt aatgcttcta cctgcagccg gccctgttct
     7681 tcttttatcg gcaggtgctg gttcagtgtg gggacgttgt ggtctgccgt tgccactact
     7741 tgttgcggcc tcagcacttt tattcccctt ttattcaaac cggtgaaagc ctgcgggctg
     7801 gtcacttcat gaataaaatg tttgtcaata tataatacag aagggccacc atcaattgtt
     7861 ttgacgatat gtttttccca gatcttttca aacagtgtct tacccatctt tatttatgca
     7921 attgctactt tagattgata ttgtttagct atcgaggcga gatctccatc ttccacttct
     7981 tttttgctat cagccacttt cacgaactca ttgtataata catctatgtc attacggtcg
     8041 aactggaaac ccagtttctg gaaacgatag gccaatgcac tgcggccact ccgggcggtc
     8101 agtacaattt ttgaactgtc ggcacctact tcctccgggt tcatgatttc atatgtttgt
     8161 gcatctttca aaaaaccatc ctggtggata ccagatgaat gcgagaatgc attactgcca
     8221 acgatcgcct tatttggttg tactggcatt cgcatggtat ccgacaccag gcggcttatg
     8281 gggtttagtt tttcagattt aattgtagtg taaagaccca ggtctttatg ttgtttgatg
     8341 atcatggcta cttcttccag ggatgtattg ccggctcgtt cgcccaaacc attaacagta
     8401 cattctattt gtcttgcacc ggcaataaca ccagcaattg aattggccgt ggctaatccc
     8461 aaatcattat ggcaatggca ggaaataata gccctgtcaa tgttgggtac attattgatc
     8521 aggtaggaaa tcttttcacc atactggtaa ggaaggcaat agcctgtagt gtctggaata
     8581 ttcactacgg ttgcaccggc tttaatcaca gcttctacaa ccctggccag gtaatcattg
     8641 tctgtccttc cggcatcttc ggcataaaat tccacatcat ctgtaaaatt tctggcccac
     8701 ttcacacact gtatagccct ctctaaaatt tcctcccgtg tagaattgaa tttggctttg
     8761 atatgcagat cagaagtgcc aattccggta tgtattctcg gacgaacagc atatttcagt
     8821 gccagagccg ccacttcaat atcattttgc acggcacggg tcaatccgca tactgtagca
     8881 ttttttatgg tctttgcgat ctggctgacc gattcaaaat cgccggggga agaaacagga
     8941 aaaccggctt cgataatatc cacacccaaa tcttctaatt gtaaggcaag ctccagcttt
     9001 tctgtggtgt tcagtttaca acccggcacc tgttccccat cacggagggt ggtgtcaaaa
     9061 atatgaactt tgccttctgc catattatta agtgaattga attagaatag aatgcaggct
     9121 gcttattttt gtattgcttg ctagtaattc agtcaataaa caacctgcaa cactaaatta
     9181 gccttgtgtg cagggcggat gaaatcaaaa taaggggtat tttacacttc ttaaccgggg
     9241 tgaattccgc tgaaaccctt acctgtattg gtatttaatt ttttttaatt acgatgccca
     9301 accgttcaac agatagcctt tttcaactgg tcaagtccct cgaaaagagt gaaaaacgaa
     9361 attttaagct ctatgtcaag cggaatacgg ccacagatga cctgaagatc atccagcttt
     9421 ttgatgcaat ggacaagatg acggactacg acgagaagca attgctgaaa aaagccaggg
     9481 gtatcagcaa acagcagttg tccaatatca aagcacattt atataaacaa attctttcca
     9541 gcctccgcct gatcaaagat gaagacaatg tagatatccg cctgcatgag caaatggacc
     9601 atgcccgcat attatataat aaaggattgt acctgcaaag cctgaaagta ctggagcggt
     9661 tgaaggaaac agccaaagca taccagcaac tgacctactg gcaacaggcg ctattctttg
     9721 agaagaaaat tgaaaccctg tttattaccc gcagtatgca ggacagggcc gataaattgt
     9781 cggctgaatc tgatggtgtg aattttcagc tgaccttagt gaaccagttt tcaaatctct
     9841 ccctgcagtt atacagctgg tatatcaaac atggccatgc ccgaaatgaa aaggatataa
     9901 agagtataga aaccttcttc agaaaacacc tgccggctga aacgcatcat gcaaagggct
     9961 tttatgaaaa attgtatctc tatcaatgtt atgtgtggta tgcatttatc cggcaggatt
    10021 ttttgcagta ttaccgctat tcgcaaaaat gggtcagtct ttttgaagaa tatccgggtc
    10081 tgctgaaagt ggaaacagca agctatataa aaggaatgca taacctgatg ggagcccagt
    10141 tcaacctggc gaaccatgaa aaactggcac agagtatcaa acattttgaa cagtttgtaa
    10201 aacaaaagca cttagccgaa aacgagaata accgtatcct ggcttaccaa tacttatatg
    10261 cggccaggat caatttgtat tttctgcagg gtactttcac caaaggtctc cggttggtcc
    10321 cttttctgga agaaatgttg aaagaatatg aactgtacct ggattctcac cgggtgctgg
    10381 tcttttatta taagatcgca tcgttgtatt ttggcagcgg taacaatgaa aaagcaattg
    10441 attatctgaa ccgcatcatt aaccagaagg gagatctacg gaccgacctt caatgctatg
    10501 cccggctgct gcatctcatc gctcattatg aacttggcaa ttttgatctg ctcgaatatc
    10561 ttatcaaatc tgtttatcgt tacatggcca ggatggaaaa tcttagtaaa gtggaagaag
    10621 ccatgtttgt ttttctgcgc cgatctttcc atgtgggagc catggcattg aaacctgagt
    10681 ttgaaaagct gctggctaaa ctgaaacaat atgaaggcaa tccattagaa gcccgtgctt
    10741 ttgcctacct cgatgtgatc tcgtggctgg aaagcaagat cagtggtgtt catgtacagg
    10801 atgtgatacg ggaaaagttt ttgttgggaa aaaggaagtt gtaagtagtt ggcaggagtt
    10861 tattcatacc gatagcagtt gggatcatcc gtttgcagat taaaaaagta acagcccctt
    10921 tcaatgggga agttatttta ctgagatatc tcttttttca tagggtcctt taagctcatc
    10981 agtatttata gcaaaatgcc gggtatagat attggtttgc tgaaaaagat tttttaacca
    11041 ggtcgctgtt tgttcgaacc cttttccatc tttcggatca aaatcgttca gcaccaaccc
    11101 gtttgcttct ttcatcatcg attgtggatc atctaaataa taatataata ccgccaggtt
    11161 aaaataactg gcatacctta ttttccggtc atgttttttg gatgatgtat aattcttttt
    11221 gatcttttcg aaataatcta ttgcaggttt taattgttct cttgctcctt caatggatgt
    11281 gcttgggttc atgctgaata atacttcatt catcacccgg aatgcttgcc ggcaggccgc
    11341 atactcagga tgtttcctgc tatcaatgat ccacatatag tcgttggctg ttacttccct
    11401 aaatccaaaa ttgtcgctga tgcgttcact caggtgatgc atggccctgt ttacacaatt
    11461 ttgtaaaagc tgtttggtaa cagtcagtga gttcacaaca aaataacctt ctgcaagggc
    11521 tcttatagta aattccgggc ttctgtatac ctgcttatga ccacggctgg ccaattcttc
    11581 gtccattata tgcatacctt tataatcggt gatgctggca ttagcggaaa aggtataaac
    11641 cacttcctgg taataataag tctttgtacc ggtgatcact ccatctctgt ttttggttgt
    11701 taccactctt tcttttactg atacaacttc cggtagcaaa tcgcctaaat ttatttttat
    11761 tgagaggtgt ccgtcttctg ctaattttcg ccaaccttcc agcattactg atttttccgg
    11821 tgacatatcc tgcagcaggg gctccatcag ctttgtgcct tccacttcta cattataagt
    11881 acggtaagta ctgtctatcc tgatttttgg gagtgagcgg tactgtactg aaaaacgata
    11941 cttgtcgagg tcgactgatt tttgtgcaga aagggcaaga ggaaacagca ataggataaa
    12001 aaagtaaagt cttttcacag gctgggattt tgttactcaa agaacggtaa aaaccggaaa
    12061 atattttaag taaaatcaca gagaattaac catacttatc attggtaaaa taccagtttt
    12121 tcattcgtat atttatcaat tgaacttttc ctgaaataac gctcattact gattttcaat
    12181 aaaggagaag acttttcttt aaaactaata tcatagtatc caacaggaag attttatctt
    12241 tagcgccagc atgaacttat ataccagcag atcggttatc attatcactt gtcccaaacg
    12301 gctggctccg tacctggcgc aggaagtact tgaattggga ttctccattg atgaaacatt
    12361 tgtaacagga gtgcggctct ccggcactat caatgattgc ataaaattaa acctgaacct
    12421 gcgctgcgcc agccaggtcc tctatagttt aaaacaattt gaagcagaaa atgcggatga
    12481 aatttataat catctaatcg cttatccatg ggaaatgatt ttgcccgacc cgggttattt
    12541 ttctattacg agcaatgtga ataatccgac catcaacaac agtatgtttg ccaatttgcg
    12601 ggtaaaagat gctattgtgg acaggatgcg ggagaaaaga ggcacccggc catcaaccgg
    12661 atcggagctt accggggcag taatccattt gttctggaaa aatgaacggg cagaaatttt
    12721 tatagatacc tccggtgatt ctttgggaag gcatgggtat cgaaaaatac ccggacaagc
    12781 tccgatgctg gaagcattag cggcagctac aatctatgct acacgttggg acaggaaatc
    12841 accttttgtg aaccccatgt gtggttcggg tacactggct attgaagcag caatgatcgc
    12901 cactaaccgg cgaccgggat tattccggac caattatgca tttatgcacc tgcagggata
    12961 cgatgaagca gtctatcagc tggaagatgc cctgttggaa aaacagatca ttgatgtacc
    13021 ggggcttcgt atcatcgcta ccgattacag tgcaaaggca gttgaaaatg ccaagaagaa
    13081 tgcggttgct gcaggtgttg ctactattat tgattttgca gtttgtgatt ttgccgctac
    13141 tgaaatacct caggatgttc ccggtgtgtt ttatgtgaat cctgaatatg gtgaacgatt
    13201 gggtgatata aaagagctgg aagcaacata tgcccgtatc ggggatttta tgaagcagaa
    13261 atgtggtgga tattatggct atgtttttac tggtaacctg gagctggcaa aaaaaatcgg
    13321 gttgaaagcc aaaagacgga tagaatttta tacgagtaca attgattgcc gcctgttgga
    13381 atatgaactg tacagcggaa gcaggaatgt tccaaaagag ataaaggaaa acacataatt
    13441 acagattgat aatactttca tactatggaa atttcagcat tgcagcaaat agtaagggaa
    13501 cgatatttta aaacagataa tgaaagaggt atttatcata cggctttatg gtttcatgaa
    13561 gaagtgggcg aattgtcttc tgctattgcc agcggggata aacagaatgc aaaggaagaa
    13621 tttgcagatg tgttgatgtg gttattaacc ctggccaacc tgatggaagt ggatatgcaa
    13681 gaagtggttg ccgactacct tgttaactcc cgtaagcttc cgtcaaaatg atggattagg
    13741 ttttattgtc atacttatga atattgaccg ggtaaataca aatgcatatt tgctctgatt
    13801 tataattgtt ttggggttga aatttataca gaaatatttc tgctatgcaa tatatgatca
    13861 tcgagaaatt tcatactggt aaggtaaagg ccatgtacca gcgttttgaa gaaagaggca
    13921 ggatgctgcc cgaaggggtt atttatatta atagttggat caatgaaact gtaactgttt
    13981 gttaccaggt aatggaaagt gaatcacctg aaaaaattca ggaatgggtc agccagtgga
    14041 atgacttggt cgattttgaa gttattcctg tcattacttc cgcaaaggca aaggaaaaag
    14101 tttttactga aatatagttc cgtttggatt ttatttcgct acggcaatct taactttttt
    14161 gttttttatt ttctgctcct taatcagctg taaggtttgg ctgactttaa ttttctttat
    14221 cgctacaaag gagaaaaagt ctttcacatc tatcaaaccg atatcttctt tttttaattt
    14281 ccctttattg gacaaaaagc cgactatatc aatcttattg actttatctt ttttcccggc
    14341 agcgataaac aaagttgacc attgtggttt atccgggagg gtagtggatt caggtaatat
    14401 aatttgttct gtatccgggt tgatataggc aggcaatttt tcttctggtg ataagataag
    14461 aatggcagta ccactggcat ccattctcgc tgttctgcca ttgcggtgag taaaaatatc
    14521 ttcgctgccc ggtaaatgat aatgaatgat atagcggata tgggcaatgt caagtcccct
    14581 tgatgcgagg tcagttgtta ctaaaacgtt tgctgttcca tttctgaatt tagataaggc
    14641 tgcatctctt tcctgttgtt ccagtgcgcc atgataaaat tcattgatga tatctttttc
    14701 tttcaggaac ttacttgttc gttctactga ttcccggtga ttacaaaaaa taattgtgga
    14761 tttgtttccc agcatacaga gtagacggaa aagtgtttcc agtttatcct ttccgtcact
    14821 cagtaatgtt ttaatagtaa gaccggtgtc ttcatctttg tcagttagaa aattaagggt
    14881 cacggaatca ttcatgccga tgaaaccggg tatctcaaca gcctgtgtgg ccgaggtcaa
    14941 tattcttttt tgtattgcgg gtaacgcacc gattataaaa gacatttcgt cgagaaaacc
    15001 taattccagt gatttatcaa attcatccag tatcagggtt gtaatggaag ccgtggtgat
    15061 atttcccctc cggatatgat ctgccaggcg accaggagtg ccaatgagta atgccggtgg
    15121 ttgttttaaa ttattttctt ctgtttccct taaatgcccg ccatagcaac aggtgacttt
    15181 caggtttgtt cccatacttt taaatacgga ttcgatctgc aaagccagtt cacgggacgg
    15241 aactatgata atagcttgtg ttttggtgtt cgccgggtcc agtaatttta taagcggcaa
    15301 caggaaggcc aatgttttac ctgatccggt tgctgataac aagagtgtat tgttatggga
    15361 gtccattgct tctattgcag catgctgcat ttcattaagg gacttgatct ttaattttaa
    15421 tagtatgctg tctgttgcat ttttttgact ggacatagtg caaaaataag gttgtttagc
    15481 ggtgtgagtg ggttgtcttc aaaattgaag tactatttca tgccaatatg acgaaaagta
    15541 gttattctca gggtttctga cttaacggct caatatttgt cacttctata tagctgtatg
    15601 gtgccatcga tttgtactga aacaaaatac ggcataagtc aacttaaatt tatttatgaa
    15661 aaagattatt ggttttttat ccctggccgc tgttttaaca actctatcct gcactgataa
    15721 acctgcggaa gttaagaagg aagtcatagt tgtgccctct tcaccaacaa ttattgtaaa
    15781 agacccacct gctaaatcta caactgtcac tttagataag aatggtgtga aagtagaatc
    15841 aaagaaagtg gatgtaacag tcaaaaagca ataatatccg ggatcactct ataaaagagt
    15901 acggagaaaa aagtgtgaat gatgtaagat attccctgca ttgtgtaaca aatagcagtc
    15961 aaaagcagtt gatatttaca taacttttat aacacggaaa gtttttctca tagagcggtt
    16021 agttttggtt aaaagccctt gctgagcttg tggaagcgtt gcaagggctt tttttacaac
    16081 tgaattcccg gctgcttccg ggtagtggtc ataattttta ttaaagaaaa t
//
LOCUS       DAORTI010000079        16044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3376_length_16044_cov_7.474889, whole genome shotgun sequence.
ACCESSION   DAORTI010000079 DAORTI010000000
VERSION     DAORTI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_16044_cov_7.474889"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1198..1986
                     /locus_tag="PLO70_10675"
     CDS             1198..1986
                     /locus_tag="PLO70_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74976.1"
                     /translation="MRKLIYIMIFISIQQNGNTQKLYSVVNLTAPFSGISYTGGHNSQ
                     EFKPSVARINLSWGVDIIYRRKKVNHKFSVEQMPFEKYFKLVNKYMLPPNTEQLLGHV
                     RINFGTAIDHFIFSYALQKEGKKEKGFLFDSKIRFNYSAGVGVSFNRSKGYYRNLYAN
                     SSDGASNQYTYMAYDAVHYRDGFGLFVRGTGGFDFVNKRGKRKLSVNLFYNQGLKDMA
                     HFDIHYRYGYWNDPAKQVDVPKLVLRSRGTVFGLSVGIPITLLK"
     gene            1992..2522
                     /locus_tag="PLO70_10680"
     CDS             1992..2522
                     /locus_tag="PLO70_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74977.1"
                     /translation="MKYYFTAVLMILLMSKPGKCQKGSVWLSGGPSMGFAVSNKNFSY
                     YYKNGIGGNLQANFGTAQLGSVTAHLSYLSFGAKNLPVTDASSSTLLKAGYQTYFLNS
                     GFFVGADAGFSFYGYGIFSGRTRFVAGATIGYSFKIAKNNYINIFPSYNQVFSTPNNN
                     KWLIANICYRFGLNKK"
     gene            2599..3429
                     /locus_tag="PLO70_10685"
     CDS             2599..3429
                     /locus_tag="PLO70_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74978.1"
                     /translation="MKIFLSILFWLIPVSAFLQVKDSILKQPGLTVSAKNSVKGASYR
                     EYKPEILTSGFIDIVNNGQVNASARFIRLFIGEPGRFAIPLSIYSGVSSNNFQNQQAV
                     GGQRSNEHLVSSFINPLSGLANMSIDGVIFFNRKAEKLTRAGFMYHGGIRILTGYKTG
                     PVSEPSTGKPINFLNSFGSTGLYFQTGAWERNNSKNVGIFWLAGRYILCKTGDKQLKE
                     IIPAIETNGVYHGWSLGWGVEINNLVNIKVLYYKYVKKPEINYSLPIYQFSFNYSLKN
                     "
     gene            3649..4311
                     /locus_tag="PLO70_10690"
     CDS             3649..4311
                     /locus_tag="PLO70_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74979.1"
                     /translation="MSTTTQQIKVAIADDHKIFRDGIRMALREKDYLKILWEAEDGKD
                     LMHKLQLKQPDVILMDIRMPHVDGINAISLIRKEYESIKIIVLTMYDDQEMITKMMEM
                     GANAYLTKTTDPEEIYQAILTCMNDDFYFNELVNKAVLLKLQHKKTVRQFYPNPVKLS
                     EKELRILKLIADDKTTEEISKEVFLSPRTIETIRQNMKQKVGAKTIAGLVMYATRNKL
                     LE"
     gene            4374..5117
                     /locus_tag="PLO70_10695"
     CDS             4374..5117
                     /locus_tag="PLO70_10695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ74980.1"
                     /translation="MSNINDTFFDGHYKEIWKTLIPDELTVKEVDFMVPHFGLQPGSK
                     VLDLMCGYGRHAIALAKKGIVVTAVDNLGDYIDEIKQVAEKDNLPLQAVKHDVITYPL
                     ETDFNLAICMGNSLNFFNAADTIRLFSNIAAHLKPGGHLLINSWSIAEIVFNKPVVNS
                     WSKIGEYKFLTEAKLSFRPTRMEMESIMIAPDGTQETKKGIDYIYSLNEMDALLQQAG
                     LHLKEVYSIPGRKKFTVGEPRAYLIAGNY"
     gene            5817..6491
                     /locus_tag="PLO70_10700"
     CDS             5817..6491
                     /locus_tag="PLO70_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ74981.1"
                     /translation="MKNPDTNSVIKVAIADDHALFRTGIKTALQARKDIQMVAEAENG
                     MQLLNLLRHIQPDVVLLDIQMPIMDGLTALPEIKKLYPDIKVIMLSFLNDHSVISKMM
                     EMGANSYITKESGAELIYEAIRGVYENDFHFNDLTNKALLSGLRTKRTNESSMPQDVQ
                     LTEKEITILKLMCDEKSTKEIADIVDLSPRTVEAIRDKLKTKTGTKSMAGLVMYAVKA
                     GLVEQA"
     gene            6562..7398
                     /locus_tag="PLO70_10705"
     CDS             6562..7398
                     /locus_tag="PLO70_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQZ74982.1"
                     /translation="MFICLNGKILRAEDPVLLASNRGYRYGDALFETMKVASKNILLE
                     AYHFERLFAGLRLLQFEVPKLLTREKLCKEVLLLAEKNHCGNLARIRLSVFRGNGGVY
                     DEERTPQYLIECWPLNESLNRLNENGLIIDVFPAARKSCDSFSHLKSANYLPYTMAAI
                     YAKANKLNDCLVLNTDGNIADATIANVFLIKEGVIITPGPDQGCVNGVMRRHLLEKMK
                     DAGYSIQENPVSVSTLEEADEVFLTNAISGIRWVKQFRDNVYSNNLTVEIYNRFIKTI
                     PA"
     gene            7416..8720
                     /locus_tag="PLO70_10710"
     CDS             7416..8720
                     /locus_tag="PLO70_10710"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQZ74983.1"
                     /translation="MKPDVTIFWFRRDLRLRDNAGLYHALKDGSPVVPVFIFDRNILD
                     ELEDKNDRRVEFIHLALQDIQQQLLKIDSSLDIRYGNPLVIYKELLKGYNIKKVFTNH
                     DYEPYAKQRDSEILQLLNEHGVSFHTYKDQVIFEKDEVLKDDGKPYTIFTPYSRKWKA
                     ALKEYHLKAYPVKKYLANFFKQPGKKIISLEEMGFAPAGLPFPGKEWKGQVIRHYKEQ
                     RDIPSVQGTSRLSVHLRFGTISIRELADEAGALNETFLNELIWRDFYHMILWHFPRVV
                     GHAFKPEYDKIRWRNNEKEFNDWCNGITGYPIVDAGMRELNQTGFMHNRVRMIVASFL
                     TKHLLIDWRWGEAYFAKKLLDFDLAANNGGWQWAAGSGCDAAPYFRVFNPYLQTKKFD
                     PELKYVRKWVPEFEELIYPQPIVAHEVARKRCLETYAAALKK"
     gene            complement(8731..10008)
                     /locus_tag="PLO70_10715"
     CDS             complement(8731..10008)
                     /locus_tag="PLO70_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74984.1"
                     /translation="MKKSIIASFTALLSFYSASAQNESVITNKLKVFIDCSNTYCDMS
                     FIRTEINLVDFMLDRIAADVHILVTEQNTGSGGDKYQLIFFGQHRFKSYRDTLQFNTD
                     PNATDFEERDLLIKYIKLGLTPFVAKTDAAKDVTISFKRTETEKDTASGITKDPWNYW
                     VYRIGTDGYLNEDAVYKNNQFSGNLSANRTTEQLKLFFSISASKNKSTFEYEDSGVIQ
                     KIIVKNKNFNAWHYLIKSINSHWSLGYEASYTKNSFSNIKGQGFFRTALEYNIFPYKE
                     VNNRYFTISYSLTARNNHYYDTTIYNEIKETLFGHRADATISFNQKWGSTSVGINYHN
                     FFHNWKLFNLGANASTNIRITGGLSFNIFISGALTRDQINLPKGGATEQEILTRRRQI
                     ASGYSIYTGFGLNYRFGSKLNNFVNPRFEGPNN"
     gene            complement(10235..11071)
                     /locus_tag="PLO70_10720"
     CDS             complement(10235..11071)
                     /locus_tag="PLO70_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HQZ74985.1"
                     /translation="MKLTLGFSPCPNDTFIFDALINKKIDTEGLEFDVVLQDVETLNQ
                     WALEGKLDITKLSFPAYFKSLEYYTLLNAGSALGKGVGPILITDSQQEITNEEINQAS
                     IALPGVNTTANLLFSFAYPEAKDKRFMLFSAIEEALVNKETDLGVIIHENRFTYQQKG
                     LRKVKDLGEYWEERMELPLPLGGIAINQWVKRSLALKVNELIRKSLDFAFKNYPLIPD
                     YVKQHSQEMSEDVMRKHIDLYVNNYSLDLGDEGKLAIESLYQVFLELNNQEADDEDVL
                     FL"
     gene            complement(11068..11721)
                     /gene="mqnB"
                     /locus_tag="PLO70_10725"
     CDS             complement(11068..11721)
                     /gene="mqnB"
                     /locus_tag="PLO70_10725"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HQZ74986.1"
                     /translation="MPNCLIVAATPLEIAPFLTYYRTKKELPEQLHIDVLLTGIGLTA
                     TTYSLTRQLAIKRPDLIIQAGVGGCFDNTMQLGSVVAVKQEAIADQSVIELNKLKTMF
                     DLELVPQDQYPFKKGWLINKSEVLKKVKLKKVKGVSVNEITTSRQKVKFYKQAFNPVI
                     ESMEGAALHYVCLMEKIPFVQIRGISNYIAERNKKNWNMKESISNLNNELIRLLKNI"
     gene            11868..12287
                     /locus_tag="PLO70_10730"
     CDS             11868..12287
                     /locus_tag="PLO70_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQZ74987.1"
                     /translation="MVYLTRIEHFNAAHKLYNPSWSKEKNDEVFGKCANENWHGHNFE
                     LFVTIKGMPDPDTGFIFDAKKLGTIVQDSIIEKLDHRNLNIDVDFMRGKLCSIENLVI
                     GIWNELEPHLPASVHLHCLKLVETSKIYVEYYGESNL"
     gene            12352..12765
                     /locus_tag="PLO70_10735"
     CDS             12352..12765
                     /locus_tag="PLO70_10735"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQZ74988.1"
                     /translation="MSKKVAVFRKEHFNAAHRLFNPSWDEERNNKIFGKCALPNYHGH
                     NYELIVKVVGEPNTNTGYVMDMKELSDIIQREIIERFDHRNLNLDTKEFKHLNPTAEN
                     IAVVIYELLRPHIDTKLDMQIRLYETPRNFVEYPV"
     gene            12784..13380
                     /gene="folE"
                     /locus_tag="PLO70_10740"
     CDS             12784..13380
                     /gene="folE"
                     /locus_tag="PLO70_10740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQZ74989.1"
                     /translation="MAYKKTEQYDEQTTTGLIKSYKEAIALLGEDPEREGLQKTPERV
                     AKAMQYLTQGYQLDAHAILNSAKFHEDVSEMIVVKDIEVYSMCEHHMLPFFGKAHVAY
                     IPNGWITGLSKVARIVDVFSRRMQVQERLTIQIKDAIKETLNPLGVAVVIEAQHLCMM
                     MRGVQKQNSVTTTSAFDGEFQKNSTRSEFMKLITAHLS"
     gene            13468..14346
                     /gene="fabD"
                     /locus_tag="PLO70_10745"
     CDS             13468..14346
                     /gene="fabD"
                     /locus_tag="PLO70_10745"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQZ74990.1"
                     /translation="MKHAFIFPGQGSQFRGMGKEHYDNSAFAKKLFEQANEILGFRIS
                     DIMFEGTDEDLKQTNVTQPAIFLHSIIAFKSIETIKPDMVAGHSLGEFSALVANGVLS
                     FEDGLHLVAARARAMQHACDKEPGTMAAILGLDDEKVETICNAIEEVVVPANYNCPGQ
                     LVISGSLKGIEIACEQLKNAGAKRALVLPVGGAFHSPLMMHAEEELIAAIEKANFYQP
                     VCPVYQNVAAKAVIRPEEIKMNLIHQLTGPVRWTQSVQSMIADGATRFTEAGPGKVLQ
                     GLVLKINKEMQVEGVN"
     gene            complement(14356..14670)
                     /locus_tag="PLO70_10750"
     CDS             complement(14356..14670)
                     /locus_tag="PLO70_10750"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ74991.1"
                     /translation="MHLFLIPTNKGLKSVRADNIIRIQAISNYCKIYFDNEYPLTVAK
                     LLLWFEGKLPEDIFYRIHRGHIVNRNFISSISCNKILTLANGEQLQISKRKKNVVKRM
                     VA"
     gene            complement(14722..>16044)
                     /locus_tag="PLO70_10755"
     CDS             complement(14722..>16044)
                     /locus_tag="PLO70_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ74992.1"
                     /translation="STTEAIASWDPNEIIGPDGAPDKSWVSINDRLPYTVTYENDISA
                     SAPAKYVKVVVPVHEKMDAATFQLGSFGFNSLTFTVPPATASYYQRLDCRDSLGLFVD
                     VIAGYDVVNNHAFWEFQSIDPVTLLAPADPLKGFLLLQDSTNQTYGHGFVNFSIKPVT
                     TAQTLDTVLASAEIIFDSNEIIPTNIEKNTIDALPPVSSINNLPASTVNTEITITYTG
                     SDDINGSGVKGYSIFVSDNGGAPELYISNFRGADTTFIGVAEHTYKFHVTATDSTGNI
                     ESLRLVDSIIVLSGEHIICPNGNVSFDSKLSGASYQWQVDTGSGFVDISDGGVYSGTS
                     TSILAITNAPSSMYGYQYRCLVNGNIYSTIFLLKFGMTWEGTISNVWENPANWSCNSL
                     PDANTDVIVNGGKSNYPQINSNVTIRTLRMNHGSTGNVNAGFTLTILK"
BASE COUNT         4837 a         3011 c         3420 g         4776 t
ORIGIN      
        1 ggagcggagt agtttattac tcttcccaaa ctaaaatacc tacatcttca tttgcatacc
       61 ttttcataac agtaacattt taagcctttt ctcaaattaa tttgacagtc aaatatggaa
      121 atccttctga cctaatacaa ttgcttttaa accgggtgat tacccttatt accttccaaa
      181 acccttaata gataaggctt taggagattt tttgtcggaa taatacttga ttaaaatggg
      241 taaaataact aagtaaaatt agtaaattta cggttgtaaa aatagtaagt ttaccgtgta
      301 aattcacggt tattaacagg tgtggataaa gaaaggaagg gtaagtggcg agtggaaaag
      361 ggaacctggt caattcaaaa ataactcaca catatttcaa aaaaaggaag ttgattgaca
      421 ttggacttcg ctatggcatt caagggattt ggaaattaca ttcaggacgg tttctaagga
      481 cattggacga aaaaataggg gctccatctt ttgtcaatca actgagtcaa aagtatatcc
      541 acatactgat gtgcacaaga gcaatactcc gggattttaa actttcagtt tttacgagtt
      601 caatcgtata gtttccgggc aggaatgcaa ttatacgata ggagaaatga aaatttacga
      661 ttatgcctca cccccttgaa acagttatcg ggtttcggca ttatttcacc ccctctccag
      721 gtggagatgg ggtggacggg cataatcttt tggcttgatt gcagacggag tgtggtaaac
      781 ttcaacagac tgtttcacaa atgttttatt ttaggatatg agtagttctg atgaatacac
      841 agaaaacctg cataagggtg ccaaagcctc aacttttgaa aatgcccggc agttgcgccg
      901 tacagaaacg gaagcggaga aaaaactttg gctgtatttg cgaaaccgcc agttgatggg
      961 caaaaaaatt caggcggcaa caagcaatcg atacttatgt actttatttt tactgtcatg
     1021 aatccaagct ggctattgaa ctggatggag ctatacataa taatgaaatg aacaggcagt
     1081 atgataatgt aagtacccct tttctcagac agcattataa tatcaaatca ggatatttat
     1141 attaaaatac cggtataact cagttcagtt aagagttata ccggttcttt tctgactatg
     1201 aggaaactta tttatataat gatctttatt tctattcagc aaaatgggaa tacccaaaag
     1261 ctgtatagtg ttgttaactt aaccgctcca ttctcaggta tatcctatac gggcgggcac
     1321 aacagccagg agtttaaacc aagcgttgcc agaataaacc tgagttgggg agtggatata
     1381 atttatcgcc ggaaaaaagt gaatcacaaa ttttcggtgg aacagatgcc atttgaaaaa
     1441 tattttaaac tggttaataa atatatgctg cctcccaata ctgagcaact tttggggcat
     1501 gtcaggataa attttggaac agcaattgat cattttattt tcagctatgc attgcaaaag
     1561 gaaggaaaaa aggaaaaagg gttcctgttt gattctaaaa taaggtttaa ctattcagca
     1621 ggtgttggtg tcagttttaa cagaagcaag ggttactaca gaaacctgta tgccaattct
     1681 tctgatggtg catcaaacca atatacttat atggcttatg atgcggttca ttatcgggat
     1741 ggtttcgggt tattcgtaag aggaactggt ggatttgatt ttgttaacaa gaggggaaaa
     1801 agaaagttat ctgttaattt attttacaac caggggttga aggacatggc ccatttcgat
     1861 attcactatc gctatggtta ttggaatgat cctgccaaac aggtagatgt gccaaaactg
     1921 gtattgcgaa gcaggggtac tgtatttggc ttaagtgttg gcatacccat tactctttta
     1981 aaataagaaa tatgaaatat tattttactg ccgtgctgat gatattgctt atgagtaagc
     2041 caggtaagtg tcaaaaaggt tctgtttggc tcagcggcgg gccttcaatg ggttttgctg
     2101 tgtccaataa gaatttcagc tattactaca aaaatggaat tggtggaaat ttgcaggcta
     2161 atttcggaac tgcccaatta ggatcagtta ctgcgcatct ttcctatttg tcttttggcg
     2221 caaaaaattt accagtaacg gatgccagtt cctcaacact tttaaaagcg gggtatcaga
     2281 cttatttttt gaactctggt ttttttgtag gagccgatgc aggtttttcc ttttatggat
     2341 atggtatatt cagtggccgt acccgttttg ttgcaggggc tactattggc tattcattta
     2401 aaatagctaa gaataattat attaatattt tcccatctta taaccaggtt ttcagtacgc
     2461 ctaacaataa taaatggctt attgctaata tttgttatcg gtttggactc aacaaaaagt
     2521 agcatacaag aaatcacttg cagttaagta tcggcttttt catgtttgtt ctaatcaaaa
     2581 gcatattcaa tttatgctat gaagattttt ttgtcaatat tattttggct gatacctgta
     2641 agtgcctttc tccaggtaaa agacagcatt cttaagcaac ccggcctgac tgtttcggca
     2701 aagaactcag taaaaggcgc ctcttacagg gaatacaaac ccgaaatcct tacttccggt
     2761 tttatagaca ttgtaaacaa cggccaggta aatgcttctg cacggtttat ccgtttattt
     2821 atcggtgagc cgggcaggtt tgccatcccg ctttctattt acagcggtgt atcttccaat
     2881 aattttcaaa accagcaagc tgttggcggg cagcggtcca acgaacatct ggtcagtagt
     2941 tttatcaatc cgctcagcgg gttggctaat atgtcaatag atggagtgat tttctttaac
     3001 cgcaaagccg aaaaattaac ccgtgccggg tttatgtatc atgggggcat ccgcattctt
     3061 acgggttata aaacaggtcc ggtttcagaa ccgtccaccg gcaagcccat caatttttta
     3121 aacagtttcg gctccacggg cctatatttt caaacaggcg cctgggagag gaacaacagt
     3181 aaaaacgtag gtattttctg gctggcaggc cgctatatcc tttgcaaaac aggcgataag
     3241 caattaaaag aaataatccc cgctatagaa accaacggag tttatcatgg ctggagcctt
     3301 ggctggggag tagagatcaa taacctcgtc aatataaaag tgctttatta caaatatgta
     3361 aagaagccgg aaataaatta ctcccttcct atctaccagt tttcatttaa ttactcattg
     3421 aaaaactgat ttacctcacc ccctcgagtc agttttgcgg attcagcatt atttcatccc
     3481 cctctccatg tggagagggg tggacgggca tattctttta atttgattat tgacggggtg
     3541 aggtaaaata cccaaaagaa atttctccat aatgcggata atactaaaat taacgtattt
     3601 ttactcaata gcgttaccca ttaaaataaa ttaaaaaacc aatatctcat gtccactaca
     3661 acccagcaaa tcaaagtggc gattgctgat gaccacaaaa tattccgtga tggcatccgc
     3721 atggcgctga gggagaaaga ttacctgaaa attctttggg aagcagaaga cggcaaggac
     3781 ctgatgcaca aactgcagct gaagcaacct gatgtgatcc ttatggacat ccggatgcca
     3841 catgtggatg gtatcaatgc catcagtctt atccgtaagg aatatgagtc catcaagatc
     3901 atcgtactga ctatgtatga tgaccaggag atgatcacca aaatgatgga aatgggagcc
     3961 aatgcctacc ttactaaaac aacagacccc gaagagatat accaggctat cctcacctgt
     4021 atgaatgatg atttttattt taatgaactg gtaaataaag cagtgctgct gaaactacag
     4081 cataaaaaga cggtacggca attttatccc aaccctgtaa agctatcaga aaaagaacta
     4141 cgtatattaa aactgatagc agatgataaa acaacagaag agatctccaa agaagtattt
     4201 ctgagccccc gcactataga aaccattcgc cagaacatga aacagaaagt aggggccaag
     4261 accatcgccg gcctggtaat gtatgccacc cggaataaat tgttagaata ggttttttgc
     4321 cgggaaatac cccggcattt ttgttgcgct aacccggttt atttaatctg cacatgagca
     4381 atatcaacga tacttttttt gatggtcatt ataaggaaat atggaagacc cttattccgg
     4441 atgaattgac tgttaaagaa gttgatttca tggttcctca ttttggcctg caaccaggaa
     4501 gtaaagtgct tgacctgatg tgcggatatg gcagacatgc cattgctttg gccaaaaaag
     4561 ggatagtggt aactgctgtt gataatctgg gagattacat tgatgaaata aaacaggtgg
     4621 cagaaaagga taatttacca ttgcaagctg taaagcatga tgtgatcact tatcctctag
     4681 agactgattt taatctggcc atttgtatgg gcaacagcct gaattttttt aatgcagcag
     4741 atacgataag actgttcagc aatatagctg cacatcttaa acccggggga catttgctga
     4801 taaacagttg gtcaattgca gaaatagtat ttaataaacc agttgttaac agctggagta
     4861 agatagggga gtataaattt cttacagaag caaaactttc ctttcgaccc accaggatgg
     4921 aaatggaaag tataatgatt gccccggatg gtacgcaaga aacaaaaaag ggtatcgatt
     4981 atatctactc actcaatgaa atggatgccc tgctgcagca ggccgggctg catctgaagg
     5041 aagtgtatag catacccgga agaaaaaaat ttacagtagg tgaaccaaga gcatacctta
     5101 ttgccgggaa ttactaaaag acatcaccaa actataaagc cacagcgatc acagagtgac
     5161 agattcattg aatacggtga atcattgtgt ccgtcttggc tgaataatag gaatgttgac
     5221 tttggcaata aatgttagaa cttaaagatt gggtgtggtc aactaaaaac cccggcgtta
     5281 tgaggaccgg ggcttgatgt tactgttatg aaaagaaaaa aatctttagg cgttgaactt
     5341 cataaggaca ggtttaacag tgttttggtt ttagtttact tttcagcagg tattggattt
     5401 ataaaacggg accattgatc aaaaaaatag aaagttgatt gtcggtgaag tgtctcccga
     5461 ttaagatcgg gttggttttt caagggaatt ggaaaattac tttctggacg gttctggaca
     5521 ttggatgaga gtaaaacaga tcatccgtat caatcaactt tctgagacaa aagtatatcc
     5581 acagtgatat gttaacaaga gcataactgc cctatttaaa acattcggta tttactggta
     5641 ctgtcgtagt ttttcaggaa tgtgatagta atccttcgtt atatagataa taaggcggta
     5701 ctttccacaa attttaaatc aagtactttc cctatggtat atattgcagg aattttccaa
     5761 cttgcagcat tgaataaata cttaatccaa taattttttt actaattatc taaatcatga
     5821 aaaaccccga cactaactct gtaattaaag tagccattgc tgacgatcat gcattattcc
     5881 gcactggtat taaaacagca ctccaggcaa gaaaagatat tcaaatggtg gctgaggcag
     5941 aaaatggaat gcaactgctt aacctgctcc gccacattca gcctgatgta gtattactgg
     6001 atatacagat gcccatcatg gatggcctta ccgctttacc agaaattaaa aaactgtacc
     6061 cggatattaa agtgatcatg ttgtcattcc tcaatgatca ttctgttatt tccaagatga
     6121 tggaaatggg tgctaattct tatatcacca aggaatcagg agctgagttg atctacgaag
     6181 cgatccgggg tgtttacgaa aatgattttc attttaatga tttaaccaat aaagcattgc
     6241 tgagtggttt gcgtactaaa cgcaccaatg aatcttccat gccacaggat gttcagttaa
     6301 cggagaagga aattactatc ctgaaactga tgtgcgatga gaaaagtacg aaagaaattg
     6361 cggacatcgt ggacctgagc ccccgtactg tggaagccat ccgtgataag ctgaaaacta
     6421 aaaccggtac caaatcaatg gcgggtttag tgatgtatgc ggtaaaggcc ggcctggtgg
     6481 aacaagcata gatttttact ggcaagcgat attcagccat tctgttttac agggtggctt
     6541 tttttatttt actttcacgg tatgttcatt tgtttaaatg gaaaaatttt acgggcggaa
     6601 gatccggtat tgctggcatc gaaccggggt taccgctatg gagatgcctt gtttgaaacg
     6661 atgaaggtag ccagcaagaa cattttactg gaggcctatc attttgaaag actctttgcc
     6721 ggcctccggc tacttcagtt tgaagttccg aaactgctta ccagggagaa attatgtaag
     6781 gaagtgttac tgctggcaga aaaaaatcat tgcgggaacc ttgcgagaat aagattgtcc
     6841 gttttccggg gcaatggcgg agtgtatgac gaagaaagaa ctccgcaata cctgatagaa
     6901 tgctggccat tgaatgaatc gctgaaccgt ttaaatgaaa atgggctgat tattgatgtt
     6961 tttcctgcgg cacgaaagag ttgtgatagt ttttcccacc tgaaatcggc taattatctt
     7021 ccttatacga tggcggcaat ctatgcgaaa gcgaataaac tgaatgattg cctggtactt
     7081 aatacggacg ggaatatcgc tgatgccaca atcgctaacg tctttttaat aaaagaaggg
     7141 gttattatta ctcccggtcc ggatcagggt tgtgtaaacg gtgtgatgag aagacacttg
     7201 ttggagaaaa tgaaagatgc cgggtacagc attcaggaaa accctgtttc tgtttcaact
     7261 cttgaagagg cggatgaggt atttcttacc aatgccatca gcggtattcg ctgggtaaaa
     7321 caattcaggg acaatgtata ttcaaataat ctgactgtgg aaatctataa ccgatttatc
     7381 aaaacaattc cggcttaggc ttgtttgcct caaatatgaa acctgacgtt actattttct
     7441 ggttcagaag agatcttcgc ctccgcgata atgcaggact gtatcatgcc ttaaaagacg
     7501 gcagtccagt agttcctgtt tttatcttcg acaggaatat ccttgatgaa ctggaagaca
     7561 aaaatgaccg tagggtagag tttattcatc ttgccctgca ggatatacag caacagttac
     7621 ttaagattga ttcatccctg gatattcgtt acggcaatcc acttgtcatt tataaggagt
     7681 tgttgaaggg atacaatatc aaaaaggttt ttaccaacca tgattatgaa ccctacgcaa
     7741 aacaaaggga ttcggagatc cttcaattat tgaatgaaca cggtgtttct tttcatactt
     7801 acaaagacca ggtgatattt gaaaaggatg aggtgctgaa agatgatggc aaaccatata
     7861 ctattttcac tccctattcg agaaaatgga aagcggcttt gaaagagtat cacctgaagg
     7921 cttacccggt aaaaaaatat ttggctaact tttttaaaca gcccgggaaa aaaataattt
     7981 cattggaaga gatgggattt gctcctgcgg gcctgccttt tcccggtaag gaatggaagg
     8041 gccaggtcat ccggcattac aaagagcaaa gagatatccc ctctgtacaa ggtacatcca
     8101 ggctgagtgt acaccttcgt tttggtacta tcagtatccg tgaactggca gatgaagcag
     8161 gcgctttaaa tgaaaccttc cttaatgaac ttatctggag ggatttttat catatgatac
     8221 tatggcattt tcccagggta gttgggcatg ctttcaaacc tgagtatgat aaaatcaggt
     8281 ggcggaacaa tgaaaaggag tttaatgact ggtgcaatgg gataacaggt tacccgattg
     8341 tagatgcggg tatgcgggag ctgaatcaga caggcttcat gcataatcgt gttcgcatga
     8401 tcgttgcatc atttttaacc aaacatttgt tgattgactg gcgctggggg gaggcttatt
     8461 ttgcaaaaaa attgctggac tttgacctgg cagcaaataa tggtggctgg caatgggctg
     8521 ctggcagtgg ttgtgacgct gcaccttatt tccgtgtttt taacccctac ctgcaaacaa
     8581 aaaaatttga ccctgaatta aagtatgtca ggaaatgggt gcctgagttt gaggaactta
     8641 tttatcccca accaatcgtt gcacatgaag ttgccaggaa aagatgcctt gaaacttatg
     8701 ccgcagccct gaagaagtaa tccatttttt ttaattattg ggtccttcaa atctcgggtt
     8761 tacaaaattg ttgagtttgg aaccaaaccg gtaattcagt ccaaaaccag tatagatact
     8821 atatcccgaa gcaatttgtc tccttcttgt tagaatttcc tgttcggtag cgccaccttt
     8881 tggaagattt atctggtccc tggtcaatgc gccagaaata aatatattga aagatagtcc
     8941 ccccgtaatt cgaatattgg tagaagcatt ggcaccaagg ttgaataatt tccagttatg
     9001 gaagaaatta tgatagttaa tgcccacaga agtggatccc catttctggt tgaatgaaat
     9061 ggtggcatct gcacggtgac cgaatagtgt ttcctttatt tcattataaa tagtagtgtc
     9121 gtaataatgg ttgttgcgtg cagtcaggct gtaactgata gtaaagtaac ggttattcac
     9181 ttccttatag gggaatatgt tgtattcaag tgcggttctg aaaaatccct gtcctttaat
     9241 attggaaaaa ctgttctttg tgtaactagc ctcatagccc aaactccaat ggctattgat
     9301 gctcttgata aggtaatgcc aggcattgaa atttttattt ttgacaatta ttttttgtat
     9361 caccccgctg tcttcgtact caaaagttga tttgttttta cttgcactga tactaaagaa
     9421 tagtttcaat tgctccgttg ttcggtttgc tgataaatta ccgctgaact ggttgttctt
     9481 gtaaacagca tcttcattca aataaccatc ggtaccaatt ctgtaaaccc aataattcca
     9541 cgggtctttt gtgatgccgg aggctgtatc tttttcggtt tctgttcttt taaaactaat
     9601 agtcacatct ttggcagcat cggtttttgc cacaaatggt gtcagcccaa gtttaatgta
     9661 tttaataagc aggtctctct cttcaaaatc agtggcgttt gggtctgtat taaattgaag
     9721 agtatcccgg tagcttttaa aacggtgctg accaaagaag atcagttggt acttatcccc
     9781 accactgcct gtattttgtt cagtaactaa aatatgaaca tccgctgcaa tacgatcaag
     9841 catgaaatct accaggttga tctcagtccg gataaaactc atgtcacaat aagtattact
     9901 gcaatcaata aagactttaa gcttattggt aataacagac tcgttctggg cagatgcaga
     9961 ataaaatgac agcaaagcag tgaaagaagc aataatgctt tttttcatga taagttgtta
    10021 aagtctctta gatcttcgta ggggtgggaa taccgaattc taatgtctga ctttaattgt
    10081 taagagggat ccaattctgg taatagcagg ataaaaggct tatacaatag aaagcaggcg
    10141 ctgaactaat ttgtaaaatt aggatttaaa ctgagaatag tgatttttca gcgttaacgg
    10201 gagaaaggca ggagcattgg attacaggtt gattttaaag aaataaaaca tcttcatcat
    10261 cggcttcctg gttatttagt tcaagaaaaa cctggtataa gctttctatg gccagttttc
    10321 cttcatcgcc aaggtcaaga ctgtaattgt ttacatacag atcaatatgc tttcgcatca
    10381 catcctcact catttcctgc gaatgttgtt taacataatc cgggattagc ggatagtttt
    10441 taaaagcgaa atcgaggctt tttcggatca attcattcac tttcaatgcc agtgatcttt
    10501 ttacccattg attgatagca ataccaccca gtggaagagg cagctccatc ctctcctccc
    10561 aatattcacc aagatctttc actttgcgca ggcctttctg ctggtaggta aaccggtttt
    10621 catgaatgat aacgccgagg tcggtttctt tattaactaa agcctcttcg atagctgaaa
    10681 agagcatgaa tcttttatct tttgcttccg ggtaggcaaa actgaaaagc aggttagctg
    10741 tcgtattaac accaggtagc gcaatggaag cctggttgat ttcctcattt gttatctcct
    10801 gctgtgagtc tgtaattaat attggcccga ctcctttccc aagggcactt cctgcattca
    10861 gcagtgtata atattctaag gatttaaagt aagcagggaa actaagcttg gtaatgtcca
    10921 gcttgccttc cagtgcccat tgattcagcg tttcaacatc ctgcaatact acgtcgaact
    10981 ccaatccctc agtatcaatt tttttattga tgagcgcatc aaaaatgaat gtatcattgg
    11041 ggcagggaga aaatcctaaa gtgagtttca tatatttttt aacaaacgga ttagttcatt
    11101 gttcaggtta ctgattgact ctttcatatt ccagttcttc ttatttcttt cagctatata
    11161 attgctgatg cccctgatct gtacaaacgg aattttttcc attaaacaga cataatgtaa
    11221 ggcagctcct tccattgatt caataacagg gttaaaagct tgtttataaa attttacttt
    11281 ctgcctggag gtggtgattt cgttgaccga cacccctttt acttttttta gtttaacttt
    11341 tttcaggacc tcactcttgt tgatcagcca tcctttttta aatgggtatt gatcctgggg
    11401 taccagttcg agatcgaaca ttgtttttaa cttattcaat tcaattacac tctgatctgc
    11461 gatagcttcc tgtttaacag ctaccactga acccagttgc atagtattat caaaacaacc
    11521 tccgacccct gcctgtatga taaggtcggg tcgttttatt gccaattgcc tggtcaggga
    11581 ataagtagtt gcagtgaggc caatacctgt aagcaacaca tcaatatgca attgttcagg
    11641 caactctttt tttgttctgt aataggtcag aaagggagcg atttccagtg gcgttgcagc
    11701 aactataagg cagttcggca taaagggaga tctgttattt gtccaaatcc ggaactgcaa
    11761 ggtaggtaaa ctatcttcta cttccggctt cagggatagc ttaattcact tacctttgcg
    11821 cctcactttt cctccgccca atgctgtgag gaactaaaaa gtaaagcatg gtttacttaa
    11881 cacgaattga gcattttaac gctgcccata aactgtataa tcccagctgg tcaaaggaaa
    11941 aaaatgatga agtgtttggc aaatgtgcca atgaaaactg gcatggccac aattttgaac
    12001 tttttgtgac tattaagggc atgcctgatc cggatacagg atttattttc gatgccaaaa
    12061 agctgggcac tatcgtccag gatagcatca tcgaaaagct tgaccatcgt aatttgaaca
    12121 tagatgttga ttttatgcgg ggtaagcttt gttctattga aaacctggta attggtatct
    12181 ggaacgaact ggagccacat cttccggctt ctgttcatct gcattgcctg aaactggtgg
    12241 agacctctaa aatttatgta gaatattacg gagaaagtaa tttatagaac aatatgactt
    12301 tattattgtt atcctttacc gtcaggctga tttatttcca taaattgtag tatgagcaaa
    12361 aaggtagctg ttttcagaaa ggagcatttt aatgcggcac atcgcctttt caacccttcg
    12421 tgggatgaag aaaggaataa taaaatattc ggtaaatgtg cgctgcccaa ttatcatggc
    12481 cataattatg aattgatcgt gaaagtggtg ggagagccta ataccaatac gggttatgta
    12541 atggatatga aagaactcag tgatattatt caacgggaaa taatagaacg gtttgatcac
    12601 cgaaacctga accttgacac caaagaattc aagcacctga atcctacggc agaaaatatt
    12661 gccgttgtta tatatgaatt attgcgtccc catattgata caaagctgga tatgcagatc
    12721 cgtttgtatg aaacaccccg aaattttgtg gagtacccgg tttaaaaaat aatacttttt
    12781 tatatggcat ataagaaaac agaacagtac gatgagcaaa caaccaccgg gttgatcaaa
    12841 agctataagg aagctatcgc cttactgggg gaagacccgg aaagggaagg gttacagaaa
    12901 actccggaga gagtagcaaa ggccatgcaa tatttaacgc aagggtacca gttggatgca
    12961 catgcaatat tgaattctgc caaatttcat gaagatgtaa gcgaaatgat tgtagtgaag
    13021 gatattgaag tgtatagtat gtgtgagcat catatgttgc ctttttttgg aaaagcacat
    13081 gtggcttata ttcctaatgg ctggattaca ggtcttagca aagtagccag aattgttgat
    13141 gtattttccc ggcgcatgca ggtacaggaa agattgacca tccagataaa agacgccatt
    13201 aaggaaacat tgaacccgtt gggagtcgct gtcgttattg aggcacaaca tttatgcatg
    13261 atgatgcggg gtgtgcaaaa acagaactca gttacaacaa cttctgcatt tgacggcgag
    13321 ttccagaaaa actcgacaag aagtgaattt atgaagctga taacggctca cctgagctga
    13381 ttgacaacga aatttaattt aatgccataa agatttttct aatcgaaacc tttatggcat
    13441 ttttgtttgt attaaccacc agttaccatg aaacatgctt ttatttttcc cggccagggt
    13501 tcgcaattcc ggggtatggg aaaagaacat tatgataata gtgcctttgc caaaaaatta
    13561 tttgaacagg cgaatgagat tttaggtttc cggatctcag atatcatgtt tgaaggtact
    13621 gatgaagatc taaagcaaac caacgtaaca caacctgcta tttttttaca ttccatcatt
    13681 gcttttaaaa gtattgaaac tatcaaaccg gatatggtgg ctggtcattc attaggtgag
    13741 ttttctgcat tggttgccaa tggggtattg agctttgaag atggcctgca cctggtagct
    13801 gcccgtgcca gggctatgca gcatgcctgt gataaggagc ccgggacaat ggcggctatt
    13861 cttggactgg atgatgaaaa agtggaaact atctgtaatg caattgagga agtggtagta
    13921 ccggccaatt ataattgtcc gggtcaatta gtcatcagtg gttctttaaa aggaattgaa
    13981 atagcctgtg aacagttgaa aaatgccggg gctaagaggg ctcttgtttt acctgtaggt
    14041 ggtgctttcc attctccatt aatgatgcat gcagaagaag aactgatagc agccatcgaa
    14101 aaagccaatt tttatcaacc ggtttgcccg gtgtatcaga atgtagcagc aaaagcagtg
    14161 atccggccgg aagaaattaa aatgaattta attcaccagc tgaccggccc cgtaagatgg
    14221 acacaaagtg tgcagtcaat gatcgcagat ggtgctacca ggtttaccga agccggtccc
    14281 ggtaaagtat tacaaggatt agtattaaaa atcaataaag agatgcaggt agaaggagtg
    14341 aactgatctg catcgtcagg caaccattct ttttactaca tttttctttc tcttactgat
    14401 ctgtaattgt tcaccatttg ccaatgtcag tattttattg caggaaatac ttgaaatgaa
    14461 attcctgttt actatatgcc ccctgtgaat acggtaaaaa atatcctccg gaagttttcc
    14521 ttcaaaccat aacagcaatt ttgccacagt aagcgggtat tcattgtcaa aatagatctt
    14581 gcagtaattg ctgatcgcct gtatgcggat gatattatct gcccgtactg atttcagacc
    14641 tttgtttgtt ggaattaaaa acaggtgcat ataattgtat ttgaaacggc tgctatactt
    14701 aaaaatatag cagccgggag gttattttaa aatggtaagt gtaaagcctg cattaacatt
    14761 gccggtggag ccgtggttca tcctgagtgt acgaatagtt acattggaat ttatctgtgg
    14821 atagttggat tttcctccat tcactatcac atcggtattt gcatctggca ggctgttgca
    14881 actccagttg gctgggtttt cccatacatt actgatagtg ccctcccagg tcattccaaa
    14941 ttttaataag aatatagtgc tgtaaatatt tccattaacc aggcacctgt actggtaacc
    15001 atacatagat gacggagcgt ttgtgatagc tagaatggat gtcgatgtgc cgctatatac
    15061 acccccatca gaaatatcaa caaagcccga ccctgtgtcc acctgccatt gataagaggc
    15121 tccggaaagt ttggaatcga acgacacatt tccgtttgga catattatat gttcaccgct
    15181 taagacgatt atactgtcca ccagtcttaa tgattcaata ttcccggtac tgtctgttgc
    15241 ggttacatga aatttatagg tatgttcagc taccccgatg aaagtggtgt cggctccacg
    15301 gaaattagaa atatataatt ccggtgcgcc accattatct gatacgaaga tagaataccc
    15361 tttcaccccg gagccattaa tgtcgtctga tccggtatag gttatcgtaa tttctgtatt
    15421 cactgtactg gccggcaggt tgttaatact gctgacaggc ggcaatgcat caattgtatt
    15481 tttttcgata ttagtaggga tgatttcgtt gctgtcaaaa ataatttctg cgctggccag
    15541 cactgtgtcc agtgtttgtg cagtggtaac cggtttgata ctaaagttga caaatccatg
    15601 tccataggtt tggtttgtgc tatcctgcaa taaaagaaaa ccttttaagg ggtcagccgg
    15661 ggcaagcaag gtaactgggt caattgactg gaactcccag aaagcatggt tgttcaccac
    15721 atcatagcct gcaataacat ccacaaataa tcccagcgaa tctctgcaat ccagtctttg
    15781 ataataggag gcagtggcag gaggtacagt aaatgtcaga ctgttgaaac caaaactacc
    15841 caactggaaa gttgccgcat ccattttttc atgtaccggc acaacaacct ttacatattt
    15901 ggcaggagca ctggcagaga tatcattttc atatgtcact gtatagggta gcctgtcgtt
    15961 tatgctcacc cagcttttat ccggtgcacc atcgggcccg atgatctcgt tagggtccca
    16021 tgatgcgatg gcttcggtag tgga
//
LOCUS       DAORTI010000080        15723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3493_length_15723_cov_5.771573, whole genome shotgun sequence.
ACCESSION   DAORTI010000080 DAORTI010000000
VERSION     DAORTI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15723
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_15723_cov_5.771573"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1472)
                     /locus_tag="PLO70_10760"
     CDS             complement(207..1472)
                     /locus_tag="PLO70_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ74993.1"
                     /translation="MKKNFLLIVCLQFLMTLAAQQADKYILLKPDRVFDGVQMQTGWV
                     VLVKNNTIEQAGAMTFKLPAGTQIIELKGHSLLPGLIEGHSHLFLHPYNETSWDDQVL
                     KESRAERTARAVNHAKATLMAGFTTVRDLGTEGAMYDDAGLKKAIEKGVIPGPRMIIA
                     TRAIVARGAYGPKSENPDVELPQGAEEVAGLEELSKAIRSQIGHGADLIKVYADYRYG
                     PNGETQPTFSEIELGWLVGNTVSSGRKAVAHASTPEGMKRAILAGVSTIEHGDDGTEE
                     VFKLMKEKGVALCPTLAAGEAVEQYRGWKKGIDPEPNRIVNKRKTFQLALQAGVTICM
                     GGDAGVYTHGDNAREMELMVDYGMKPLEVLRSTTSVNADVFGYASKIGRIKKGLAADI
                     IAVEGDPSADIKNIRKVNLVIKDGVIYKK"
     gene            complement(1480..1767)
                     /locus_tag="PLO70_10765"
     CDS             complement(1480..1767)
                     /locus_tag="PLO70_10765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ74994.1"
                     /translation="MLIHHKQVGSKGMFFIGTEGAILAEMVYTSPSADKMIIEHTEVS
                     DELRGQNIGFQLVNTAVEYARKHHISIIPLCPFANSVFKKKPEFADVLHTS"
     gene            2039..3451
                     /locus_tag="PLO70_10770"
     CDS             2039..3451
                     /locus_tag="PLO70_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQZ74995.1"
                     /translation="MKKIEINTTCKRMLADVFTPVGIYLRLRDKYRDTILLESTDHHS
                     TENSYSFICINAIGGIEISAADSIEFKLPGQQPRKLVLKNKKEVPQLLWDFMQRFDAK
                     KTAIKEGKFAQGLYGYTSYDAVQFFESIQFSKTTLQGVGVLRYRLYQYVIAINHFKDE
                     LFICENKVAGVESDVATVESLIRSKDVPVYPFSPKGEESSNMSDAAYIEMVKKGIASC
                     HRGDVFQIVLSRRFRQPFTGDEFNVYRALRSINPSPYLFFFDYGDYKLMGSSPEAQLV
                     IQNGKAVVHPIAGTFKRTGDDEADREATQRLLEDAKENAEHVMLVDLARNDLSRVCDE
                     VTVTHYRQVQYFSHVIHLVSEVTGKLKEGANPFELLAKTFPAGTLSGAPKFKAMELID
                     AYEPTARSYYGGAIGFMGFDGSCNHAIMIRTFLSRQNILTYQAGAGVVAASNPESELQ
                     EVNNKLGALKKAIEMGAEIS"
     gene            3516..4088
                     /locus_tag="PLO70_10775"
     CDS             3516..4088
                     /locus_tag="PLO70_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQZ74996.1"
                     /translation="MRILVFDNYDSFTYNLVHLVEKITHTKVEVYRNDEIPLEKVKEY
                     DKIILSPGPGIPEEAGLLLPLIKEYAASKSILGVCLGHQAIGQAFGGKLVNLSTVYHG
                     VATPVKVDEQKKSPLFVGLPPVIEVGRYHSWIISDKGFPDELEVTARDENDYIMALQH
                     KEFDVQGVQFHPESVLTPLGEEILRNWLKK"
     gene            4209..5207
                     /gene="trpD"
                     /locus_tag="PLO70_10780"
     CDS             4209..5207
                     /gene="trpD"
                     /locus_tag="PLO70_10780"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQZ74997.1"
                     /translation="MKKILQYLFEHKTLSRDAAKDVLVNIGKGMYNEHEVTAFMTVYL
                     MRSITIEELQGFRDALLELSVKVDLDGYETMDIVGTGGDGKNTFNISTLACFIVAGTG
                     QKVAKHGNYGASSISGASNVMEQLGYRFSTDNAKLKNEIEEANICFLHAPLFHPALKT
                     VGPIRKNLAMRTFFNMLGPMANPANPAFQLVGVYNLEMARIYNYLLQLGGKPFTIIHS
                     LDGYDEISLTNDTKVITNEGERIMTPEQLGKRMVSATDISGGNTVEEAAKIFTTILKG
                     DGTWAQNAVVLANASMALHCTGKFNSYDDAYHAAVNSLESGKANECLRKLISIQVA"
     gene            5610..6401
                     /gene="trpC"
                     /locus_tag="PLO70_10785"
     CDS             5610..6401
                     /gene="trpC"
                     /locus_tag="PLO70_10785"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQZ74998.1"
                     /translation="MDILEKIIAAKRKEIEKFKPLSSIERFEREGFFWQIRTRSLVQS
                     LLLPGSTGIIAEFKRKSPSKGWFKTKELETEPVVTAYNKTAAGISVLTDDEFFGGDMD
                     DLIQTKVVTDIPVLRKDFIIDKWQIAEAKAFGADVILLIAACLAPARVKDFASYAQSI
                     GLESILEVHNEEELGHCCDEVSMVGVNNRNLKTFEVDINTSLQLIDKIPAGKPAIAES
                     GISSVDTIVTLRNAGFKGFLIGENFMKEEHPGKAFEEFVYNLKQL"
     gene            6401..7030
                     /locus_tag="PLO70_10790"
     CDS             6401..7030
                     /locus_tag="PLO70_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQZ74999.1"
                     /translation="MNIKVCGITEMKQLQQLDGLDIDFAGLIFYKESPRYIGDKISRE
                     DLKRADLDLKKVGVFVNPEMIEVLDAIDEYGLDAVQLHGDESPEMCEDLSSEVEVIKA
                     FRIKEGVTDIDALVAAYDAVCDYYLFDKAIDNSFGGTGKQFDWSILTKAKIEKPFFLS
                     GGIGVEDAVKIKAFKHPDFFGVDINSQFEKSPGVKDMGMVLQFKQALKK"
     gene            7032..8219
                     /gene="trpB"
                     /locus_tag="PLO70_10795"
     CDS             7032..8219
                     /gene="trpB"
                     /locus_tag="PLO70_10795"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQZ75000.1"
                     /translation="MSVITYQQPNEQGYYGSFGGAYIPEMLHRNVEELRTKYLEIIYE
                     EGFQKEFQQLLRDYVGRPTPLYLAERLSKRYGAVIYLKREDLCHTGAHKINNTIGQIL
                     LAQRLGKKRIIAETGAGQHGVATATVCALKGVECIVYMGEKDIERQAPNVARMKMLGA
                     TVIPATSGSKTLKDATNEAIRDWINHPDDTHYIIGSVVGPHPYPDMVARFQSVISEEM
                     RKQLLEKTGNELPDYVIACVGGGSNAAGAFYHFLEEPSVKLIAVEAAGEGINSGKSAA
                     TTQLGTPGILHGSKSLLMQTHDGQVVEPHSISAGLDYPGIGPLHAHLFKTGRATFMSA
                     TDNKALNAAFECAKMEGIIPALESAHALHALKMINFNKNAVVVICLSGRGDKDLDMYM
                     KAL"
     gene            8295..8678
                     /locus_tag="PLO70_10800"
     CDS             8295..8678
                     /locus_tag="PLO70_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HQZ75001.1"
                     /translation="MFYGASQVIFKRAEELRKNQTEDEKLLWVYLKSNQLGVRFKRQH
                     PIWMYIADFYCHELKLVIELDGSIHTVKDVIDNDKEREEDIKSFGIRVVRFTNKELRA
                     DIAKVIDRIKCIIKEIKEHGTDSEK"
     gene            8656..9471
                     /gene="trpA"
                     /locus_tag="PLO70_10805"
     CDS             8656..9471
                     /gene="trpA"
                     /locus_tag="PLO70_10805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQZ75002.1"
                     /translation="MEQIARSRISRVFGSLPFRGDLEGPGRGILNVYCTAGYPRLHST
                     LEVMKALQDNGANIIELGMPYSDPLADGPVIQHSSAVALANGMTMAILFEQLRDFRKV
                     ISIPVVLMGYMNPVLQYGFEKFCADVAAVGIDGLILPDLPEYEFESEYGAIIKKYDLD
                     FIFLVTPETAEDRIKQLDSLSAGFLYAVSSSSTTGSNKQTVDYSQYLGRLRSLDLKNP
                     VLVGFGINDKASFDAVCRLAEGAIIGSAYIKALENSADVNASTKSFLSSILGQ"
     gene            9553..10650
                     /locus_tag="PLO70_10810"
     CDS             9553..10650
                     /locus_tag="PLO70_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ75003.1"
                     /translation="MKKAVFLLLVAPLFVFAQPKPKSFTINGKLDGYPDGTEIRLVKN
                     GENTELSSAKLLKSKFVLKGSIPEAVLCFLFIGDEQKPAEIYVENKIISFKGDKAKPG
                     DYKIEGSASHTDFANFVKEFVPMAQQLSSLANTLNYTVPGPQRDSLMVTYTSLQGSIQ
                     GVIDKMVKDKPKSLVTAFVLGATYGFNEDPIQLEQRFNKLDAVVRKSEAGKQLEVQIA
                     DSKIGAVGTMAMEFTQPDTTGTPVALSSFRGKYVLVDFWASWCGPCRQENPNVVETFN
                     KFNTKNFTVLGVSLDRPGQKSKWIDAIHEDKLTWTHVSDLKFWDNEVAKLYKVQSIPQ
                     NILLDPDGKIIAKNLRGPALEAKLCEILGCN"
     gene            complement(10746..11852)
                     /locus_tag="PLO70_10815"
     CDS             complement(10746..11852)
                     /locus_tag="PLO70_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ75004.1"
                     /translation="MKKILILLATAGLPLLSVAQIPLTVLPSGGNKKASVSERIGLTD
                     VTIHYDRPGVKGREGKIWGQLVPVGFTDPGFGSSKSSPWRAGANENTTFEFSTDVKIE
                     GQALPAGKYGFFIAYDPGECTLIFSKNASSWGHYYYDAKEDALRVKVKGQPLDKSVDW
                     LKYEFSDQKENSAVVSLQWEKLAIPFTIEVDYINTQIASFRKELRTEKGFIWQSWDQA
                     AGWCAERNVNLEEALRWTDSSTHNFGGEKSFTAWGTRAQVLNKLGRNEEAAATIKKAL
                     PLGSMNEIHQYGRQLLGMKLFQQAFDIFKMNYDKNPDQYTTLMGMTRAYSGLGDFKNA
                     ARYGTMALAKAPDEVNKKYTQGLIDKLKEGKDIK"
     gene            12047..14893
                     /locus_tag="PLO70_10820"
     CDS             12047..14893
                     /locus_tag="PLO70_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQZ75005.1"
                     /translation="MKILFVCLLISYALQSPAQILNRIKDRAAGKARSEASNAKYNAK
                     NKVREAGRKELDDFNAQFDSTDIDYAILLSDNSGVFGGRGKGEFGAKFIRLAGIANSL
                     IKDADLSDEENARLNLQLGQSAYATGRYVYAEKRLTAAKNYFEKGYLTSDLGYLKTIS
                     GHGLLYTAMGRFAQAEKFTTDALQLRRDKMGEDDMSVAASYNNYAVLHYNLGQYNESE
                     KEFESALSIIAANNQKESMPYAIIVNNKAILYQSMGRYEEGAKLLEEALHLAGRLEVT
                     KAKNHLKFFSNLALLYQQMGKYAEAERIYQGLEKRLEKGKPEYANMMNNLAILYLVMK
                     KDDLVEDLLRRSAHIYKTAMGETSPAYAKVVSDLGNFLRYKGRYAEATPMLEGVLQNR
                     EQILGTDHPLYVQSEEDLAILWWKKKEYAKAYTLYRDVMEKSLTFINRYFPPMSEAEK
                     AKYWDILSVRFQRFYNFSLEAAATNNTIIADLFEYRLATKGLLLSSVRKVSESILQSG
                     NEQLVKNYLTWIDLKEQLTTLYAYSKEELKEQSVNLDSLQAAANAMEKKLSESSKEFS
                     QFYFTSKISFAEIQGQLKPEEALVEIIRLRNYDQVLTDSCRYIGLVVTKEQPEPKLVL
                     LTNGEAMEGKIAKAYRLSMKNKISDEKSYTHYWAPFEPELKGKKKIFVSPDGVYSQLS
                     LYTLKQPGGDFLINKYDIVLLGNPKDVLARNNPADPSIKKATLIGFPEYSSASIPQLP
                     ATKTEVDGINKVLRSSGYQVAEFIQKDATEVNLKATRKISILHIATHGYFLKDVEKAS
                     WPIGVHADNAKENVLLRSGLMLSGAAEGDKESASLENKSNGVITSYEAMNLDLQGTHL
                     VVLSACETGLGEVRAGEGVYGLQRAFLVAGAEALVMSLWKVDDAATQQLMNNFYTNWT
                     KTGDKQKAFKQAQLQLMTKYKEPFYWGAFVMMED"
     gene            complement(15000..>15723)
                     /gene="rsgA"
                     /locus_tag="PLO70_10825"
     CDS             complement(15000..>15723)
                     /gene="rsgA"
                     /locus_tag="PLO70_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQZ75006.1"
                     /translation="LLVQAADRDFNINRLERYLTICNSSKVSPIIVLTKIDLIDEHRT
                     FEILEKIKARINNVPIIAISNESKDGYDKIKAIIEKGKTYCMLGSSGVGKSTLLNNLS
                     GKTIMRTDTISQSTNKGKHITSHRELIILENGGILVDNPGMREVGVADSTSGLEITFD
                     RIIRLSQHCKFKDCTHTNEIGCSVIEAVEKGEIDQASYENYLKLEREKAHFESSVIER
                     RKKDKEFGKMVKNYKKYINRKK"
BASE COUNT         4675 a         2982 c         3698 g         4368 t
ORIGIN      
        1 attgtaaggg ataaaaaaaa caggaaggaa aggaagtatc ttttcaatac cataaaaata
       61 attttaaggc aacaagttac atccaaaaaa gcttgctgcc atcggggtgt tgtaaattgt
      121 ttggagtgta caccaggcta tttccagcta tccggcagcc tgttgctgat ccgaaagagt
      181 agttggaaac tgaacagtaa gctggtttat tttttataga taaccccgtc tttgatcacc
      241 aagttaactt tgcggatatt ttttatatct gctgaagggt ctccttctac ggcaataata
      301 tctgcggcca gtcctttttt aatcctgccg atcttactgg catagccaaa tacatctgca
      361 ttgacagatg tggttgatct cagcacctct aatggcttca taccatagtc caccatcaat
      421 tccatttctc tggcattatc accatgtgta tatacaccgg catctccgcc catacagata
      481 gtgacacctg cctgcaaagc cagttgaaat gtttttcttt tattaacgat gcggttgggt
      541 tcgggatcaa ttcctttttt ccaacccctg tattgttcta ccgcttcacc ggctgccagt
      601 gtgggacaaa gtgccactcc tttttctttc atcagtttaa acacttcttc ggtgccatcg
      661 tcaccatgtt caatcgtaga tacaccagca agaatggctc ttttcattcc ttccggtgta
      721 ctggcatgtg ctactgcttt cctgccgctg cttacggtgt ttcccactaa ccaacccaat
      781 tcaatttcac tgaaagtagg ttgcgtttca ccattgggcc cataccggta gtcagcatat
      841 actttgatca ggtctgcacc atgaccgatc tgcgaacgga tggctttgct cagttcttcc
      901 aggcctgcta cttcttcagc gccctgcggc agttctacat cagggttttc acttttgggt
      961 ccgtatgctc cccgggcaac tattgctctt gtggcaatga tcattcttgg tccgggtata
     1021 actccttttt caattgcttt tttcaaaccg gcatcatcat acatagcgcc ttctgttccc
     1081 aggtccctga cggttgtaaa tcctgccatc agtgttgctt tggcatgatt gacagctctt
     1141 gcagttcttt ccgcccttga ttcttttaat acctggtcat cccagcttgt ttcattatag
     1201 ggatgaagaa ataaatgaga atggccttca attaagccgg gcaacaagct gtggcctttt
     1261 aattctatga tctgtgttcc tgcaggcaat ttgaaggtca tggcaccggc ctgttcaata
     1321 gtattatttt ttaccagtac tacccagccg gtctgcattt gtacaccatc aaatacacgg
     1381 tcaggcttca gtaaaatata tttatcagcc tgctgtgccg caagtgtcat taagaattgt
     1441 aaacaaacga tcagtaaaaa attctttttc atgttattgt tatgaagtgt gaagtacatc
     1501 agcaaattca ggtttctttt taaaaactga attggcgaac ggacataaag gtattatgct
     1561 gatatggtgc ttccgggcat attcaacagc ggtattcacc aattgaaagc cgatattttg
     1621 tccccggagt tcatcgctta cttctgtatg ttcgatgatc attttatcag ctgatggcga
     1681 cgtgtacacc atttcggcca ggatggcacc ttctgtacct atgaagaaca ttcctttgga
     1741 cccaacctgt ttgtgatgaa tgagcatgga gcaagttttg ataaaactaa caagattttt
     1801 ttgctaatct gaattcagca ccacatcttt gtaaggaaat gaaaatgata caaacaaata
     1861 tttggtggtg gcatacaact cttccggggc tgtaatgtca ttgttatatt tgtttaaata
     1921 tattcaggcc ccgacagtgt tcggggcttt taatttgtcc ctaacgccag aaggggggaa
     1981 acggggccac caatgaaaaa ataaagtcct tccttcgggg aggatgcagg aggaaagcat
     2041 gaagaaaata gaaataaata ctacttgtaa gagaatgctg gcagatgtgt ttacaccagt
     2101 tggtatttac ctgcggctgc gggataaata ccgggatacg atactgctgg aaagtactga
     2161 ccatcattcc accgaaaaca gttattcctt tatctgtatc aatgccattg ggggaataga
     2221 gattagtgct gcagatagta ttgaatttaa attaccgggt cagcaaccca ggaagctggt
     2281 tttgaaaaat aagaaggaag tgccacagct cttgtgggat tttatgcaac gatttgatgc
     2341 aaaaaaaact gctataaagg aaggaaaatt tgcacagggt ctttatggat atacttcgta
     2401 tgatgcggta caattttttg agtcaataca attctccaaa accaccctcc agggagtagg
     2461 ggtgcttcgg tatcgtcttt atcaatatgt cattgccatc aatcatttca aggatgaatt
     2521 atttatctgt gagaataaag tggcaggagt ggagagtgat gtggcaacgg tagaatcgct
     2581 gatacgaagt aaagatgttc ctgtgtatcc attttcaccc aaaggggaag aaagttctaa
     2641 tatgagtgat gcggcttata tagaaatggt aaaaaaagga attgccagtt gccatcgcgg
     2701 tgatgtattc cagatcgttc tcagcaggag atttcgacag ccatttaccg gggatgaatt
     2761 taatgtgtac agggctttgc gcagcatcaa cccgtcgcct tatttattct tttttgatta
     2821 tggtgattac aaactgatgg gttcttcacc cgaagcacag ctggtgatac agaatggaaa
     2881 agcagtggtg catcctattg caggaacatt taaacggacc ggtgatgatg aggcagacag
     2941 ggaagccacc caacgattac tggaagatgc aaaagaaaat gcggagcatg tcatgctggt
     3001 ggacctggcc aggaatgacc tgagccgggt gtgtgatgag gttacagtta cacattaccg
     3061 gcaggtacaa tatttttcgc atgtaattca cctggtaagt gaagtaactg ggaaactgaa
     3121 agaaggggca aacccttttg agctgttggc taaaacattt ccggccggca ctttaagcgg
     3181 tgccccgaag tttaaagcga tggagctgat cgatgcatat gagcccacgg cgagaagtta
     3241 ttacggaggt gccattggtt tcatgggctt tgacggcagt tgcaatcatg ctattatgat
     3301 cagaacattc ctgagccggc aaaatatatt gacttaccag gcaggcgccg gggtggtagc
     3361 ggccagtaac ccggagagtg aattgcagga agtgaataat aagttaggag ctttgaagaa
     3421 agcgatagag atgggtgccg aaattagtta acaggttgac aagttgctaa gtaatatgct
     3481 aatcaacctg tcaacaattc aacttgtaaa ttgaaatgag aatacttgta tttgataact
     3541 acgattcttt tacttacaat cttgttcacc tggtggaaaa gataacacat acaaaagtgg
     3601 aggtgtaccg caatgacgag atccctctgg aaaaagtaaa agagtatgat aagatcattt
     3661 tatcgcccgg tccgggaata ccggaagaag caggactgtt attgcctttg ataaaagaat
     3721 atgctgcttc caaatctata ttgggagttt gtttgggcca ccaggcgatt ggccaggcat
     3781 ttggcggcaa actggtgaac ctgtcaacgg tttatcatgg tgtggccaca ccggtaaaag
     3841 ttgatgagca aaagaagagc cctttatttg taggattacc acctgtaatt gaagtgggac
     3901 gttatcacag ctggatcata agcgataaag gatttcctga tgaactggaa gtgacggcaa
     3961 gagatgagaa tgattatatc atggcattac agcataaaga atttgatgtg cagggagtgc
     4021 agtttcaccc ggagagtgta ctgacaccgt tgggagagga gattttgaga aactggctga
     4081 agaaataatt caagtaaaaa taaattccgt gcccttgtgt cctggtgcct cagtgtttca
     4141 aaccttttta tgttgtaaca caaagacact aaagcacgga gacagaaaag aagaataagg
     4201 aaagaagtat gaaaaagatt ttgcaatatt tatttgaaca caaaacactt tcaagagatg
     4261 cggcaaaaga tgtgttagtg aacattggta aaggcatgta taacgaacat gaagtaacag
     4321 cttttatgac ggtgtacctg atgcgcagta tcactattga agagttgcag ggctttcggg
     4381 atgcattgct ggaattatcc gtgaaggttg acctggatgg ctatgaaaca atggatattg
     4441 tgggtaccgg tggtgatgga aaaaatacat ttaatatttc aaccctggcc tgttttattg
     4501 tggcaggaac aggtcaaaaa gtggcgaagc acggcaatta tggagcttct tctatcagtg
     4561 gagccagcaa cgtgatggaa cagttgggat atagattcag tactgataat gcgaagctga
     4621 agaatgaaat tgaggaagcc aatatctgtt ttttgcatgc tcccttgttt catccggcgc
     4681 tgaaaacagt cgggccgatc aggaagaacc tcgccatgcg gacattcttt aatatgctgg
     4741 gaccgatggc caatcctgca aatcccgctt ttcagctggt aggagtttat aacctggaga
     4801 tggcaaggat atataattac ctgttgcaac tgggtggtaa accttttact attattcaca
     4861 gcctggatgg atacgatgaa atttcgctga cgaatgatac caaagtgata acaaatgaag
     4921 gagaaagaat aatgacgccg gaacaattag gtaagcggat ggtatcggca acagatatca
     4981 gtggggggaa tacagttgaa gaggcagcga aaatatttac aacgatattg aaaggagatg
     5041 gaacctgggc gcagaatgct gtggtattgg ccaatgcgtc aatggcatta cattgcaccg
     5101 ggaaatttaa ttcgtatgat gacgcatatc atgcggcggt gaatagtttg gaaagtggaa
     5161 aggcaaatga atgtttacga aaactgattt caattcaagt tgcctgaatc cccgcaaaat
     5221 ggactttagt ttttgatgat ttgatgcagg atagattcta tgaatgttat tgaggtgtga
     5281 tattgcagcc ccgaaggggc gaaatattga tagccccgga tgaaatccgg ggtaaacaat
     5341 agaaatgatt tttcagtgtt ccgaagaggc gaaatgttgg tagccccgga tgaaatcagt
     5401 ggtaaacaat agaaatgatg tttcagtgtt ctgaaggggc gaaatgtttg taaccccgga
     5461 tgaaatccgg ggtaaacaat agaaatgatg tttcagtgtt tcagtgttcc gaaggggcga
     5521 aatgttgata gccccggatg aaatcggggt aaaagataaa aatgattttt tgagagctcc
     5581 gcaggagcga tatattttaa agtgatcaaa tggatatttt agaaaaaatt atcgccgcta
     5641 agcggaaaga aatagaaaag ttcaaaccgt taagcagtat tgaacggttt gaaagagaag
     5701 gattcttctg gcagatcaga acccggtcgc tggtgcaatc tttattgtta cccggcagta
     5761 cgggtatcat tgcggagttc aaacgaaaaa gcccgagcaa gggatggttc aagactaaag
     5821 agctcgaaac agaacctgtt gttacagcgt ataataaaac tgcggcagga atttctgtat
     5881 taacggatga tgaatttttt ggcggagaca tggatgattt gattcagaca aaagtggtga
     5941 cagatattcc tgtgctgcga aaagatttta tcatcgataa atggcagatc gctgaagcga
     6001 aggcctttgg ggcagatgtt attttattga tcgctgcctg cctggctccg gcccgggtga
     6061 aggattttgc ttcgtatgca caaagtatcg gcctcgagtc aatactggaa gtccataatg
     6121 aagaagaact gggtcattgt tgcgatgaag taagtatggt gggagtgaat aaccgtaacc
     6181 tgaagacgtt tgaagtagat attaatacct cgctgcaact gatagataag ataccggccg
     6241 gtaaaccggc tattgcggaa agtgggatca gcagcgtgga tactattgta actttgcgaa
     6301 atgccggttt caaaggtttc ctgatcgggg agaattttat gaaagaagaa cacccgggaa
     6361 aagcatttga agaatttgta tataatctaa aacaactata atgaatatta aagtttgtgg
     6421 tatcactgaa atgaagcaac tgcagcaact ggacggattg gatattgatt ttgcaggatt
     6481 gatattttat aaggaatcgc cacgatatat tggagataaa atttccaggg aggatctgaa
     6541 aagggccgat cttgatctta aaaaagtagg tgtgtttgta aacccggaga tgattgaagt
     6601 gctggatgcg attgatgagt atgggttaga tgcagtgcaa ttgcatggcg atgaaagccc
     6661 ggagatgtgc gaagacctga gcagcgaagt ggaagtgata aaagcatttc gtattaaaga
     6721 gggtgtgact gatatcgatg cattggtggc tgcgtatgat gccgtatgtg attattactt
     6781 gtttgacaaa gcaattgata acagtttcgg cggtaccggc aaacaatttg actggagtat
     6841 tttaaccaaa gcgaagattg aaaaaccttt tttcctgagt ggtggcattg gggtggaaga
     6901 tgcggtaaaa ataaaagcat tcaaacatcc tgactttttt ggtgttgata taaacagcca
     6961 gtttgaaaaa agccccggag taaaggatat gggaatggtg ctgcagttta aacaagcttt
     7021 aaagaaataa aatgtctgta attacatatc aacaaccaaa tgaacaaggt tactacggtt
     7081 cttttggcgg ggcttatatt cccgaaatgc tgcaccgcaa tgtggaagag cttcggacaa
     7141 agtacctgga gataatctat gaagaaggct ttcaaaaaga gtttcagcag ttattgcggg
     7201 attatgtggg caggccaaca ccattgtatc tggcagagcg gttaagtaaa cgatacggcg
     7261 ctgttattta cctgaagcgg gaagaccttt gtcataccgg cgctcataag ataaataata
     7321 cgatcgggca gatattgctg gcgcaacggt taggaaaaaa aagaatcata gcagaaacag
     7381 gggctggtca gcatggcgtg gctaccgcta ctgtatgtgc attgaaaggg gtagagtgca
     7441 ttgtgtacat gggagaaaag gatatagaac ggcaggcgcc gaatgtggcg agaatgaaaa
     7501 tgctgggtgc aacggtgatc ccggcgacca gtggaagcaa gaccctgaag gatgcaacga
     7561 atgaagccat ccgggattgg atcaatcacc cggatgatac acattatatc attggctcag
     7621 tagtggggcc acatccctac ccggatatgg tggctaggtt ccagagtgtc atcagtgaag
     7681 agatgcgtaa acaattgctg gagaagaccg gcaatgaatt acccgattat gtgattgcct
     7741 gtgtgggtgg tggtagtaat gcggcagggg ccttttatca tttcctggag gaaccttcgg
     7801 tgaagttgat tgcagtggaa gcagcagggg aaggtatcaa cagcggcaaa agtgcagcaa
     7861 caacgcagct cggtacgccg ggcattttgc atggcagtaa aagtttgctg atgcaaaccc
     7921 atgatgggca ggtagtggag ccgcatagta tttcagccgg attggattat ccgggcattg
     7981 gccctttgca tgcacattta tttaaaacag gccgggccac tttcatgagt gctaccgata
     8041 acaaagcact gaatgccgcc tttgaatgtg ccaaaatgga aggcatcatc cccgcactgg
     8101 aatcagctca tgctttgcat gccctgaaga tgatcaactt taataaaaat gccgtagtgg
     8161 tgatttgttt gagtgggagg ggggataagg acttggatat gtacatgaag gccctctaaa
     8221 tctccctaaa gggagacttt ggactaagat tgttttgatt ttccttaatg attgacaatt
     8281 agcttatggg gaaaatgttt tatggtgcgt ctcaggtaat tttcaaaaga gctgaggagc
     8341 tacgaaaaaa tcaaacagag gatgagaaac tattgtgggt gtatctcaaa tcaaaccagc
     8401 ttggcgtaag attcaaaagg cagcacccta tttggatgta tatagcagat ttttattgcc
     8461 acgaattaaa actggtgatt gaattagacg gttctattca tacagtaaag gatgtgatag
     8521 ataatgataa agaaagagag gaagacataa aatcttttgg aatacgagtc gtaagattta
     8581 ccaacaaaga attaagggct gatatagcta aagtgattga caggataaag tgtataataa
     8641 aagaaattaa agaacatgga acagatagcg agaagtagaa ttagcagagt ctttggaagt
     8701 ctccccttca ggggagattt agaggggcct gggaggggta ttttaaatgt ttactgtacc
     8761 gcaggctatc cccgtttgca ttcaacgctg gaagtaatga aagcactgca ggataatggt
     8821 gctaatatta ttgaactcgg catgccttac agtgatccgc tggctgatgg cccggtgata
     8881 cagcatagca gtgctgttgc attagcaaac ggaatgacga tggcaatact gtttgaacaa
     8941 ttaagggatt tccgaaaagt aatttccata ccggtagtat taatgggata tatgaaccct
     9001 gttttacaat acgggtttga aaaattttgt gcggatgttg cggctgttgg tatcgatgga
     9061 ctgatcttac ctgatttacc ggagtatgag tttgaatcgg aatacggcgc tatcatcaaa
     9121 aaatatgatc ttgattttat ttttctggtt acaccggaaa ctgctgaaga taggatcaaa
     9181 caattggaca gcctgagtgc cggcttttta tatgccgtct cttcttcttc gaccaccggc
     9241 agtaataaac agacggttga ttacagtcaa tacctcggca ggctcaggag tttggatctt
     9301 aagaacccgg tgctggttgg ctttggaata aatgacaaag cttctttcga tgccgtttgc
     9361 cggctggctg aaggtgccat catcggcagc gcctatatta aagcgctgga aaacagtgca
     9421 gatgttaatg catccacaaa atcctttctc tcgtccatat tgggtcaata aggtttctgc
     9481 caaccagctc ctttcattaa atttgtacac taattttttc cctgtatcat ttatgaatac
     9541 taattaccaa atatgaaaaa agcagtcttt cttttattag tagctccctt gtttgttttt
     9601 gcacaaccca aaccaaaaag ttttaccatc aatggaaaac tggacggata ccctgacggt
     9661 acagagatcc gtttggtaaa gaatggtgag aacacagaat tgtcttcggc aaaactgctt
     9721 aaatcaaaat ttgtattgaa aggcagtatc cccgaggcgg tcctttgttt tttgtttatc
     9781 ggtgatgaac aaaagccggc cgagatctat gtggagaata agatcatctc cttcaaaggt
     9841 gataaagcaa aacccggcga ctataagatc gaaggttctg cttcacatac agactttgca
     9901 aactttgtaa aggaatttgt acccatggct cagcaactga gttcattggc aaatacttta
     9961 aattatacag tgcccgggcc gcagcgggat tcattaatgg ttacttatac gtctttgcag
    10021 ggaagtatcc agggggtaat cgataagatg gtgaaggata aacctaaatc attggttacg
    10081 gcatttgtat tgggagcaac ttatggtttt aacgaagatc ccattcaact ggagcaacgg
    10141 tttaataaac ttgatgcagt cgtccgcaaa tctgaagccg ggaagcaact ggaagttcag
    10201 atcgctgaca gcaaaattgg tgctgtcggt actatggcga tggagtttac gcaaccggat
    10261 acaacgggta cacccgtagc cctttcctct ttccgcggta aatatgtact ggttgatttc
    10321 tgggccagct ggtgtggtcc ctgccggcag gaaaacccga atgtagtaga gaccttcaat
    10381 aagttcaata caaaaaattt tactgtgctc ggtgtgtcat tggacagacc cggacaaaaa
    10441 agtaagtgga tagatgctat acatgaagac aaacttacct ggacacatgt aagtgacctt
    10501 aaattctggg ataatgaagt agcaaaactt tataaagtgc agagtattcc tcagaatata
    10561 ctgcttgatc ccgatggtaa gatcatcgcc aagaacctga gaggaccggc gcttgaggcc
    10621 aagctgtgtg agatattggg ttgtaactga taaatcaaat tatcatcaat aacgaaagtc
    10681 cggaaggaac atcatataac aggatagttc ttccggattt tttacttaca ggttgttgag
    10741 cttttttact ttatatcttt cccttcttta agtttatcaa tcaatccctg tgtatatttt
    10801 ttattgactt catccggtgc ttttgccaaa gccattgtgc catacctggc ggcattttta
    10861 aaatcaccca atcctgaata agctcttgtc attcccatca gtgtggtgta ctggtccgga
    10921 ttcttatcat agttcatttt gaatatgtca aaagcctgct ggaataattt catgccaagt
    10981 aactgacggc cgtattgatg tatctcgttc atgctaccca agggcaatgc tttttttatt
    11041 gttgcagcag cttcctcatt ccttcccagt ttattcaata cctgtgcacg ggtaccccac
    11101 gcagtaaaac tcttttcgcc gccgaaatta tgggtagaag aatcagtcca gcgaagcgcc
    11161 tcttccaggt ttacattccg ttccgcacac cagccggcag cctggtccca gctttgccag
    11221 ataaatcctt tctctgttct cagttctttc cggaatgagg cgatctgtgt gtttatataa
    11281 tccacttcta tggtaaaggg gatggctaat ttttcccatt gcaatgaaac aacggcacta
    11341 ttttctttct gatcgctgaa ttcatatttc aaccaatcca ccgatttatc caggggctgc
    11401 cctttcactt ttacccgtaa tgcatcttct ttggcatcgt aataataatg tccccaggaa
    11461 gaagcatttt ttgaaaagat aagagtgcat tcacccggat catacgcaat aaagaatcca
    11521 tatttaccgg caggtaaagc ttgcccttct atcttcacat cggttgaaaa ttcaaacgta
    11581 gtgttctcat tggcgcctgc ccgccaggga gatgatttgc tgctcccgaa acccggatca
    11641 gtaaaaccaa ccggtaccag ttgtccccat atttttcctt cacggccttt tacacccggc
    11701 cggtcataat gaatagttac atccgttaaa ccaattcgtt cacttaccga agcttttttg
    11761 ttgccgccgc tcggcaaaac agttaaagga atttgtgcaa cagataaaag gggaagcccg
    11821 gctgttgcaa gcaatatcaa aatctttttc ataacctgcg ttttaatata ctcaaactac
    11881 cactgaaaac tgaagaacaa aaagttatat taccaatgac aaatggaacg gatgagttgc
    11941 agtggcaaag gatatgaggt gtaaaccggc ttaccggcac cgcgaattac aaattatcgt
    12001 aacttcctga acggatctta tcttcattca cctcaaatag ctggtcatga aaattctttt
    12061 tgtatgcctg ctgattagtt acgctctgca atctcctgca cagatattaa ataggataaa
    12121 agacagggct gccggcaaag caaggtcgga agccagcaat gccaaataca acgcaaagaa
    12181 taaagtaagg gaagctggcc gtaaagaact ggatgatttc aatgcacagt tcgactctac
    12241 cgatattgac tatgctatcc tgctgagtga taattcagga gtatttggcg gaaggggtaa
    12301 aggtgaattt ggtgcaaaat ttattcggtt ggcaggtatt gccaattcac tgatcaaaga
    12361 tgctgacctt tctgatgaag aaaatgcaag gttgaacctg cagttgggac aatcggccta
    12421 tgccaccggc cgctatgtct atgcagaaaa aagactgacg gctgccaaaa actattttga
    12481 aaaaggatat ctcaccagtg accttggtta cctgaaaact atttcaggcc atgggttatt
    12541 atatacagcg atgggacgtt ttgcacaggc ggaaaaattt acaacagatg cgttgcagct
    12601 gcgccgcgat aaaatgggag aggatgatat gagtgtcgct gcttcgtaca ataattatgc
    12661 agtgttgcac tataaccttg gtcagtataa tgaatccgaa aaggaattcg aatcagccct
    12721 ttccatcatt gcggctaata accagaaaga atcaatgccg tatgccataa tagtaaacaa
    12781 taaagctatt ctttatcaat ccatgggccg ctacgaagaa ggagcaaaac tgctggaaga
    12841 agccttgcac ctggctggca ggctggaagt aacgaaggcg aagaaccacc tgaagttttt
    12901 ttccaacctg gcactgctct accagcaaat gggaaaatat gccgaggcag agcgaattta
    12961 ccaggggctg gaaaaaagat tggaaaaagg aaaaccggaa tatgccaaca tgatgaataa
    13021 cctggccatt ctttacctgg tgatgaaaaa ggatgacctg gtggaggacc tgctgaggcg
    13081 ttcagcacat atctataaaa cagcaatggg agaaacaagc cctgcttatg caaaagtggt
    13141 cagcgacctt gggaattttt tgcggtataa aggccgttat gctgaagcaa cgcccatgct
    13201 ggagggggta ttgcaaaaca gggagcagat tttaggaact gatcaccctt tgtatgttca
    13261 atcggaagaa gacctggcaa tcttatggtg gaagaaaaag gagtatgcaa aagcctatac
    13321 gttatacagg gatgtgatgg aaaaatcgct gaccttcatc aaccgctatt ttccgccgat
    13381 gagtgaagca gagaaagcaa agtactggga tatcctgtcg gtgcggtttc agcggtttta
    13441 taatttttct ttagaagcag ctgctaccaa taatacaatt atcgctgacc tgttcgaata
    13501 caggctggca accaagggcc tgttgcttag ttcagtcaga aaagtgagtg aaagtatttt
    13561 acaaagcggt aatgaacaac tggtgaaaaa ttatcttacc tggatcgatc tgaaagaaca
    13621 gctgacaact ttgtatgcgt attcaaaaga agaattgaaa gaacagagtg ttaaccttga
    13681 ttcattacag gcggctgcta atgcgatgga gaaaaagttg tcagagagtt caaaggaatt
    13741 ttcgcagttc tattttacca gcaaaatatc tttcgcagag atacaaggtc agttaaaacc
    13801 agaggaagcc cttgttgaaa ttatccggtt aaggaattac gaccaggtgc ttaccgattc
    13861 atgtcgttat attggattgg tggtaacaaa agagcaacca gaaccaaagc tggtactgtt
    13921 gactaatggc gaagcgatgg aaggcaaaat tgccaaagcc taccgcttgt cgatgaagaa
    13981 taagataagc gatgaaaaat cctatacgca ttattgggcg ccatttgaac cggagttaaa
    14041 agggaaaaag aaaatatttg tttcaccgga tggagtgtac agccagctaa gcctgtatac
    14101 cctgaaacaa cccggtggcg actttcttat caacaaatat gatattgttt tactcggtaa
    14161 tccaaaagat gtgttggcca ggaacaatcc tgctgatcca tccattaaaa aagcaacact
    14221 gatcggtttt cctgagtaca gttctgcatc catcccgcag ttaccggcca ccaaaacaga
    14281 agtggatggg atcaataaag tgttgcgatc atccggttac caggtggcgg agttcattca
    14341 aaaggatgcc acagaagtta atcttaaagc aaccaggaag atctccatcc tgcacattgc
    14401 cacgcatggt tatttcctga aagatgtgga gaaagccagc tggcccatcg gggtgcatgc
    14461 agataatgca aaagaaaatg tattgctccg ttcgggtttg atgctgagcg gtgctgccga
    14521 aggggataaa gaatctgcca gcctggaaaa taaaagcaat ggggtcatta cctcatatga
    14581 agcgatgaat cttgatttgc agggtaccca cctcgtggta ctgagtgcct gcgaaacagg
    14641 attgggagaa gtaagggcag gagagggagt atatggtttg caacgggcct tcctggtggc
    14701 gggagcagaa gccctcgtta tgagtttgtg gaaagtggat gatgcagcca cccagcaatt
    14761 gatgaataac ttttatacca actggacaaa gacaggggat aaacaaaaag cttttaagca
    14821 ggcacaactg caactgatga cgaaatacaa agagccattt tactggggag catttgtgat
    14881 gatggaagat tgagaagcag tgtaaacagt tacacactgc agggctgtat aatgatcaaa
    14941 ttttacttgg gtgttttgct cagttcatgt taacggttag gacttttttt cttaggactt
    15001 cattttttcc tattgatgta ctttttgtaa ttctttacca tttttccgaa ttctttatcc
    15061 tttttccttc tttcaataac cgaagattca aaatgggctt tctctctttc caatttcagg
    15121 taattttcat atgaagcctg gtctatttct cccttttcaa cggcttcaat gacagaacaa
    15181 cctatttcat ttgtgtgagt acaatctttg aacttacaat gctgagaaag cctgatgatc
    15241 ctatcaaatg tgatttctaa tccgcttgtt gaatcagcaa cacctacttc tctcattcct
    15301 ggattatcaa ctaatattcc tccattttca agaataatta attctctgtg acttgtaatg
    15361 tgtttccctt tatttgtact ctggctaatt gtatctgttc gcattatagt tttcccagaa
    15421 agattattta ataaagtaga ttttccaaca cctgaagaac caagcatgca atatgtttta
    15481 cctttttcaa taatcgcttt tattttatcg tacccgtctt ttgattcgtt acttattgca
    15541 ataatcggaa cgttattaat ccgggcttta attttttcca atatttcaaa agtccggtgt
    15601 tcatcaatta aatctatttt agtaagcaca ataatggggc taacctttga tgaattacaa
    15661 atggtcaggt atctttcaag tctattaata ttaaaatccc tgtcagcggc ttgcaccaat
    15721 aat
//
LOCUS       DAORTI010000081        15637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3534_length_15637_cov_5.795597, whole genome shotgun sequence.
ACCESSION   DAORTI010000081 DAORTI010000000
VERSION     DAORTI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_15637_cov_5.795597"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLO70_10830"
     CDS             complement(<1..1009)
                     /locus_tag="PLO70_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HQZ75007.1"
                     /translation="MPTEIKCPNCGHQFEPNDAIREEVEKELRSKAADWQKKKNEEFQ
                     NKMEEEKKRLQQSLEENIRKNIAADFENKLKMLEQNNKDNEEKLKQARQQQLDFLRKE
                     QELINKEAELELSVQKKLQEEREKLSIEIRKLEEQRNAAKETEFQLRLKEMEEKLEAQ
                     RKLAEEMRRKAEQGSMQSQGEVQELLLEEMLRTAFPFDLIAEVGKGVRGADCIQTVRN
                     SFGQECGKIIFESKRTKDFSNDWIEKLKADMRSQGADVAVIITQALPKDMTNFGEKEG
                     VWICSFAEAKAVVHMLRDAVIKIFNASKSQENKGDKMHLLYGYLTSREFAEQWQAIRE
                     GF"
     gene            complement(1425..1510)
                     /locus_tag="PLO70_10835"
     tRNA            complement(1425..1510)
                     /locus_tag="PLO70_10835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Leu,seq:taa)
     gene            complement(1557..1629)
                     /locus_tag="PLO70_10840"
     tRNA            complement(1557..1629)
                     /locus_tag="PLO70_10840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1594..1596),aa:Gly,seq:gcc)
     gene            complement(1730..1948)
                     /locus_tag="PLO70_10845"
     CDS             complement(1730..1948)
                     /locus_tag="PLO70_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75008.1"
                     /translation="MTHNQEYHEELQKVQDKDFAHNWVSSSGFLFYLQVACIVAFVLG
                     TCTVLYNKRYSKIEVPVQESSQYTPKYK"
     gene            complement(1993..3024)
                     /locus_tag="PLO70_10850"
     CDS             complement(1993..3024)
                     /locus_tag="PLO70_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQZ75009.1"
                     /translation="MNPLPKVAIVILNWNGQKYLEQFLPFVVATHYDNFEVIVADNGS
                     ADSSVDFLQKNYPGIRIICFKKNYGFAKGYNEALKLVKSDYYVLLNSDVEVHPNWLAP
                     MVDLLESNSNIAACQPKVLSYKNKKMFEYAGAAGGWLDKYGYPFAKGRIFDICEEDKG
                     QYDQSEPIFWASGASLFIRSSVFHEVKGFDEYFFAHQEEIDMCWRIQLAGYKIYSCPA
                     AVVYHVGGGTLPRGNSLKTYLNFRNNRIMLSKNLPYSKKIWIVPARNFLDALSAWKGL
                     LSGDGGYFIAIIRSHLSYTKWWFFYQHKSVFPVTKKGNLSGYSKKNMIWQHFIKKRKH
                     FDEIMDETK"
     gene            3086..4237
                     /locus_tag="PLO70_10855"
     CDS             3086..4237
                     /locus_tag="PLO70_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQZ75010.1"
                     /translation="MFRQILNWRTLLALVAIIIVSGTIFYSQYLAKKIAREERQKVEQ
                     WVEAGKFLINAPINADITFASRIVTENKSIPIIETNEKDSITNYINLDSAQVAKNSNY
                     VEQKLRQFKSQNKAVEWTDPQNPSIRNRYYYGESKLLNEVRYYPIVQLCIVALFIAIT
                     LLSLRSSYRSVQNQVWAGMAKETAHQLGTPVSSLEGWVEMLKEKPGNEKIAQELEKDV
                     DRLRLVSDRFGKIGSTPHLEETNLVSQINNMVDYMRKRAAGKITFTVNAHGQQQIVSR
                     VSAPLFDWVIENLLKNALDAMEGKGAITVDIHEDAKSVHIDITDTGKGISKQNTARVF
                     KPGFTTKKRGWGLGLSLSRRIIKHYHKGEIFVKHSELGKGTTFRIVLKK"
     gene            4285..4368
                     /locus_tag="PLO70_10860"
     tRNA            4285..4368
                     /locus_tag="PLO70_10860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4319..4321,aa:Leu,seq:gag)
     gene            complement(4396..5214)
                     /locus_tag="PLO70_10865"
     CDS             complement(4396..5214)
                     /locus_tag="PLO70_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQZ75011.1"
                     /translation="MNNANPILINWRKEQKVFDENLSLLRKKLDKEAIHDLRVAIKKL
                     RAYLELYSQLKKVTDPVEAGLPGLLDKTEELFDTIGRQRDVEICMEVLTSFKEEINYS
                     YPQFTLYLQAVLKITKAWSNQEIHRYKNKETKKIAFVLKQDNEIENTGELNQNIQSII
                     RHSAGETKSQFKKPHLLRKNLKSVYYWINIVNDNSEYHPELLHNILDDLGSWQDKEIA
                     LQRIKHFRKDYLPKSFEEYNKLKELEKYIDEKKRMLIKAALNKTRKWIKTIPLS"
     gene            complement(5315..6172)
                     /locus_tag="PLO70_10870"
     CDS             complement(5315..6172)
                     /locus_tag="PLO70_10870"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HQZ75012.1"
                     /translation="MKILLLPVTLLTSLALQAQYYYNDIIGTQETNNLMKSYVANKVK
                     TVSATGYDNRGVKATDFSEFQEVKENGRVLKNSSINNFNKTVVYSRFDEKGRVINITD
                     SSTAIQSSTTYTYDATGRVSQILNVVKDSASDFDHSETHQWFYSASGKPEKMWRILNN
                     TGAENTIDSLEVRFITDEDGNTAEEKTYKKGVETSYLYYYYDDRNRLLDIVRYNTRLR
                     KLMPDIMFEYDEKDNIAQKTTTTSSLHLGYLIWRYIYDEKGLKTKEVLFNNDKQITGK
                     IEFTYTFGQ"
     gene            6275..7351
                     /locus_tag="PLO70_10875"
     CDS             6275..7351
                     /locus_tag="PLO70_10875"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQZ75013.1"
                     /translation="MSHIPQRKDKDCLNCGTTVQGNYCQNCGQENVVPHETFWHMVKH
                     FLYDITHFDSKFFDSMKFLLLRPGFLPQEYIKGRRASYLNPVKKYVFTSAVFFLIFFG
                     FFKGGDVVKINLATELTREQRANYLKRGEQELLKDTASEMWKEALQVLKDTSKVLTGE
                     DMIKYWDDIEFINLSGKKYTNLKEYDSIQKAIPAVERDGWLQRTIQRKNLKLKEKYRS
                     DPGKGFNTLGNTFLHKLPYLLFASLPLFALILKLLYIRRKNFYYADHAIFSIYHYIFS
                     FILLLFVFGLDKLSTVTGLEVFDALTGLLFLSGGVYLYISMKRFYGQRHMKTFFKFLL
                     LNVSGIIMLVVLFAAFILFSVFEI"
     gene            7360..8172
                     /gene="mazG"
                     /locus_tag="PLO70_10880"
     CDS             7360..8172
                     /gene="mazG"
                     /locus_tag="PLO70_10880"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQZ75014.1"
                     /translation="MPTKNENAFTRLVEIMDDLREKCPWDKKQTIQSLRQMTIEETYE
                     LADAITEEDWKGVKEELGDLLLHIVFYAKIGTEQQQFELHEVITGICEKLVARHPHIY
                     GDVKVENEEDVKRNWEKLKLKEGKKSVLSGVPQSLPATVKAMRLQEKAKQVGFEWDNK
                     EQVWEKVEEELQELKSAVVSREANKMGGDIEKLQKDVEDELGDVLFSLINYARFLQVD
                     AENALERTNKKFIYRFTQMEQQAIQQGKNLNDMTLTEMDAIWNTIKQQRPNK"
     gene            8169..11933
                     /locus_tag="PLO70_10885"
     CDS             8169..11933
                     /locus_tag="PLO70_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQZ75015.1"
                     /translation="MKPQLKNIFWSKFSFLVLAVLFFTLSFVFNKLYSSRTSVTREAE
                     LAETFIRNQQKDFTDLLTDTALIRRLLNKKESLSELTNLSARNYGIFLYSVNEFYDVT
                     MKFWSDQLIVPPPEMLSAEDGEYFTQLSNGYYYIIKKSLGSDGNSAGTLAFAMIPVWS
                     EFFIETDYLPQKFTYSNTAARWVRISDAVTEFPVKTFSGKTLFYLDKKVSGAVPFNDL
                     LTLFLRFGALIFLFLFIHLAAESTSKKYGPWKSIALLVFFLLSLRLVSYFFPSLLNLR
                     QFEIFNPVIYGADAVQRSLGDLLINALLLCWVVLFAWSKLRYIENPVSGLSLKLRWLL
                     GLLALCTLIYSSFVLANVVRSLVSDSKISFDVTNFFSLDWYTVAGFVVLACLSLSYYY
                     LSQLLFRFIFPLFAGRNYFIYFSIGFTGLVYLTFRSGNPEVLFYLPVLLWLLIYTWLI
                     HRQGIIFKRIRINIGGMLSWLFIFSVSVAVIMLSENKKVEWEKRKRMADVLSVQTDKS
                     SEMLMSIAIQYLDNDFLRENFNRFSDPENGKEFRDSIVTDNYRGYLNKYDTRLYVYDE
                     YNRPLYNEDATSYESLNTILSVQSKPTQIEGLFYYETSYDKFNYITRREVRDTANKKI
                     GSFFIVSNPKNFSNEALFPELFRQFKKSDPESSPIYSIAVYAGRRLMSPPGNYPFPIW
                     LIDVEVPKEEFLQRTNGDFDELWHRSGNDKVVVIARKKETFIQAITLFSYIFCSFLFM
                     VAFVKLVSLVLKIGTNWAGIRRLFQFNIRRQVHSTIIFISIFSFLVIGAATISFFINR
                     YKENNSEKLSRTMKIMVNEMEKKMADHNTFDDVIKIYDSVSNTGLQGLVDEVSEIHGV
                     DVNVYDLSGNLQVSSEANVYTKGVLSKKIDPVAYYYLDKLRKVQHLQTERVGNFSYYS
                     MYSPVRDADGQAYAYINIPYFTSQPELEKEISNFLVTIINLNAFIFLIAGLIAMFITN
                     RITDSFSLISDKMKEVNLGKVNEKISWSRNDEIGELVHEYNKMVAKLGESAAALAKSE
                     REGAWREMARQVAHEIKNPLTPMKLSIQYLQKAINNNQPNVKELSANVANTLVEQIDH
                     LSKIAADFSQFANIGNTNVSTFDLHDVIGSLRELYGSDENVRFVWRPLKDKVFVQADK
                     TQMNRLFTNLFANAVEACNGNSFCLIEVNESRQENILRISIKDNGEGIDPEMQERIFV
                     PNFTTKSSGTGLGLAMCKGIVEQAKGNIWFETSQGKGTTFHVELPIMDNL"
     gene            12041..13027
                     /locus_tag="PLO70_10890"
     CDS             12041..13027
                     /locus_tag="PLO70_10890"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HQZ75016.1"
                     /translation="MKKQSLLVVLSFFLLSVNAQVYKTNGPLVHTYSIVARDSVTGEM
                     AVAVQSHWFSVGTAVSWAEAGVGAVATQSFVNKSFGPRGLALVKSGLTAQQALDSLLA
                     DDEGREVRQVAIVDNKGNVATHTGSGCIQYASHVKGSQFSVQSNMMLGNKVPVAMKEA
                     FEKSTGKPLGERMLLALEAAQGAGGDIRGQQSAALLIVPGSSAGRPWDERTVDLRVDD
                     HKEPVKELRRLYTISIAYEQMNKGDLAVEKNDMAVAMNEYNAAMKMMPGNLEMQYWTA
                     VTLANNKELNKSLSIFRKVFAKDKNWKELTRRLPAVKLLTVSEEALKAILAL"
     gene            13244..13492
                     /locus_tag="PLO70_10895"
     CDS             13244..13492
                     /locus_tag="PLO70_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75017.1"
                     /translation="MKNQTKFVVSPNMVPSNASITVQSPVNMFAADSYHIVNDRGELV
                     RKGKISDPAREFSLSVGGMPDGIYWLVMGELRERFTIV"
     gene            complement(13502..13954)
                     /locus_tag="PLO70_10900"
     CDS             complement(13502..13954)
                     /locus_tag="PLO70_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75018.1"
                     /translation="MHIRKFSIAVITISLSAVSCQPGVTDQKEKAKQEINMAEKEFEK
                     MAAEKGIAEAFWFFADSIAVIKRENDTLITGKENIKKYYSAEYFKSASVKWSPDFTDA
                     ADNGDMGYTYGKYVWQSKDSTGNTNEARGVFHTVWRKQADGSWKYVWD"
     gene            complement(14018..14356)
                     /locus_tag="PLO70_10905"
     CDS             complement(14018..14356)
                     /locus_tag="PLO70_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75019.1"
                     /translation="MNTVQHSEFLHLANSLQQRGLSYDEITLQLKEKGATENLLQETI
                     QQLKNLRLIKKRNTGFACCGIGVALLVVGCMLTILLYSSGGDIKWAMYGLTTIGVIFT
                     IKGMADIMGW"
     gene            complement(14411..15460)
                     /locus_tag="PLO70_10910"
     CDS             complement(14411..15460)
                     /locus_tag="PLO70_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75020.1"
                     /translation="MRVSLYFFLSFLLLTLSCKQKENKKEKENPEAVIISELTEKTAC
                     LLSQIAYCTNIDSAINKYMPGWKPIWEAAELGGNHAFVASNGREFALAIRGSLINFSW
                     AAFQNWIYQDLHVASQEKWTFTNDSSNAKLAQGAFDGWQNLCRMTDKKTGQLLLPLLE
                     TVLKNNAPLLITGHSLGGNLAVTYASYLWQIFKTKQHLVPNINVITFAAPATGNEAFA
                     NDFNKKFPASIRVENKNDIVPKFPSAAKITSLGSLYNDSLSAGKIEVGYKNLTVSLST
                     VFTMLKAAMVLLEFTSGISPYVQTNGNGKQVNIPLSGKNTGNDILNWLGEAGYQHSIA
                     QYAVYEGVPLVECTQ"
BASE COUNT         4477 a         2992 c         3268 g         4900 t
ORIGIN      
        1 tgaatccctc ccggatggcc tgccattgtt cggcaaattc acgactggtc aggtagccat
       61 aaagcaggtg cattttatca cccttgttct cctggctttt tgacgcatta aatatcttaa
      121 ttactgcatc ccgtaacata tgaacaacag ctttcgcctc tgcaaacgaa cagatccaaa
      181 ctccctcttt ctcaccaaag ttggtcatat ctttaggtaa tgcctgggta ataattacag
      241 ccacatcagc accctggctt cgcatatcgg cttttaattt ttcaatccag tcattggaaa
      301 aatcttttgt tcgtttactt tcaaaaatga ttttaccaca ttcttgtcca aaactattac
      361 gaacagtttg aatacaatct gccccgcgga cgcctttgcc aacttctgca atcaggtcaa
      421 atgggaaggc agttcgcagc atttcttcca gcagcagttc ctgtacttct ccctgtgatt
      481 gcatactgcc ctgctccgct tttcgtctca tttcttcagc cagtttccgc tgcgcttcca
      541 gtttctcctc catttctttc aggcgcaact gaaattctgt ttctttcgca gcattccgtt
      601 gttcttccag ctttcttatt tcgatggaaa gtttctctct ttcttcctgt agtttcttct
      661 gaacagataa ttccagttcc gcttctttat taataagctc ttgctctttc cttaggaaat
      721 ccagttgttg ttgcctggct tgttttaatt tttcttcatt atccttatta ttttgttcca
      781 gcattttcag tttgttctca aaatcggcag caatattttt gcggatattt tcttcgaggc
      841 tttgttgtag tcgcttcttt tcctcttcca ttttattttg aaactcttca ttctttttct
      901 tctgccagtc tgctgctttg ctgcggagtt ctttttctac ttcctcccgg atcgcatcat
      961 ttggttcaaa ctggtgacca caattcggac attttatttc agtaggcatt aattcggctt
     1021 tagtttcaaa tttacaaata aaaaaagcga ctcattttat tgaatcgctt ttaaaagtgc
     1081 cccggacccc gattgcatcg gggtaggcca ttgccgggta aattacctac ttagggtaat
     1141 ttggccagtt cccggcaata agcgactcta tataaattct gcttttttgt ttctttattc
     1201 ttaattcttc ttttctggcc ccggcctccg tctcaaatgg cttaaaagca caaagtttat
     1261 atgggataca atttcttgta gctggaacga tacccttatt atgtctgttc agccttactt
     1321 ccacatcagc actaaaacca acataataac ggttacattt ttctgagtat agtatgtata
     1381 caaagtacat aaaataaaaa agcgacttgc gtcgctttta aaagtgcccc ggatcggagt
     1441 tgaaccgata cggccattgc tggccacagg attttaagtc ctgcgtgtct accaattcca
     1501 ccaccagggc aaccggtaaa aaaaatcctc cgccggaacg gcaagaggac tttctgagcg
     1561 gaagaccggg ctcgaaccgg ccaccccgac cttggcaagg tcgtgctcta ccaaatgagc
     1621 tacttccgca ttttaaaaag aactgctggt cccttgttgc cctcattttt tctgaaggcg
     1681 ggacgcaaaa atagggcaag tgcccttttt aaaaaaattt ttgaactaat tatttgtatt
     1741 tcggtgtgta ctggctgctc tcctgcacag gaacttcaat cttgctgtat cgtttattat
     1801 acaatacggt gcaagtgcct aaaacaaagg caacaatgca tgctacctgc aggtaaaaaa
     1861 gaaaacctga agatgaaacc cagttgtggg caaaatcctt gtcttgtact ttttgaagct
     1921 cttcgtggta ttcctggtta tgggtcatac tagatttttg tgttgcaaaa ataagggttc
     1981 aaaccaaaaa aatcattttg tttcatccat aatttcatca aaatgctttc tctttttaat
     2041 aaaatgctgc caaatcatgt tctttttaga gtagcctgaa agattacctt ttttggttac
     2101 cggaaataca cttttgtgct gataaaaaaa ccaccacttt gtataagaaa ggtgggaacg
     2161 gattattgct ataaaataac cgccatctcc tgacagtaat cctttccagg cagacagtgc
     2221 atcgaggaaa tttcttgccg gtacaatcca aatttttttg gaatagggta ggtttttgga
     2281 aagcattatc ctgttattcc tgaaattgag ataggtcttc aatgagttac ccctgggtaa
     2341 agtgccacca ccaacatggt acactactgc agccgggcag gagtaaattt tatacccggc
     2401 cagttgtatt ctccagcaca tgtcaatttc ttcctggtgt gcaaagaaat attcatcaaa
     2461 ccctttcacc tcgtgaaaga cagaagaacg gataaataac gatgccccgc tggcccaaaa
     2521 aattggctct gattggtcgt actggccttt atcttcctca catatatcaa agattctccc
     2581 tttggcaaag gggtaaccat atttatctaa ccagcccccg gccgccccgg catattcaaa
     2641 cattttttta ttcttatagg aaagaacctt tggctggcag gccgctatat tggaattgct
     2701 ttccaataag tcaaccattg gcgcaagcca gttgggatgt acttctacat ccgaattcag
     2761 cagcacataa taatctgatt tcaccagttt caatgcttca ttataacctt tggcaaaacc
     2821 atagttcttt ttaaagcaga tgatacggat cccgggataa tttttttgga gaaagtcaac
     2881 cgagctgtct gcagatccat tgtcggccac aatcacttca aaattgtcat aatgggtggc
     2941 tacaacaaaa ggtaaaaact gttcaaggta tttttgtccg ttccagttca gtattactat
     3001 ggctacttta ggtaaggggt tcaagggagt gaaaatattt attcaaaaat aagggaagcc
     3061 ggtttatcct cgttaaattc gaaacatgtt taggcaaatt ttaaactgga gaacattgct
     3121 tgctttggtt gccattatta ttgtaagcgg tactatcttt tattcacaat acctggctaa
     3181 aaaaatagcc agggaagagc ggcaaaaagt ggagcaatgg gtagaagcgg ggaaattcct
     3241 tatcaatgcg cctattaatg ctgatatcac ttttgcctca agaatagtta cagaaaataa
     3301 atcgataccc attattgaaa ccaatgaaaa agacagcatc actaactata ttaacctgga
     3361 ttcagcacag gtagcaaaga atagtaacta tgttgagcaa aagctccggc aatttaagtc
     3421 tcagaataag gctgttgaat ggacggaccc gcaaaaccct tcaatccgca acaggtatta
     3481 ttatggtgaa tccaaattat tgaatgaggt ccgttattac ccgatagttc agttatgcat
     3541 tgtagcacta tttatagcaa ttacattgtt gtcattacga agtagctatc gttctgttca
     3601 aaaccaggta tgggcaggta tggcaaagga aactgctcac cagttgggaa ctcctgtttc
     3661 ttcgctggaa ggctgggtgg agatgctgaa agaaaaaccc ggaaacgaaa aaatagcaca
     3721 agagctggaa aaggacgtag acaggctgcg tttagtatct gacaggtttg gtaaaatcgg
     3781 cagtactccg cacctggaag aaaccaacct ggtgagccag atcaataata tggtggatta
     3841 catgcgaaaa agagctgccg gtaagatcac atttactgta aatgctcatg ggcagcaaca
     3901 aatagtaagc cgggtgtcgg ctcctttgtt tgattgggtg attgaaaatt tattgaagaa
     3961 tgcgctggac gctatggaag ggaagggggc catcacagta gatattcatg aagatgcaaa
     4021 atcggttcat attgatatta cagataccgg caaagggatc agtaaacaaa atacggcaag
     4081 agtgttcaag ccggggttta ctacaaaaaa aaggggatgg gggcttggct taagtctttc
     4141 ccggcgtatc attaagcatt atcataaagg agagattttt gtaaaacact cagaattggg
     4201 aaaaggaact acgttcagaa ttgttctgaa aaaataaaca gcttacattt gcagtcctta
     4261 atcccgaatt attgataaag ggatgcccgg gtggcgaaat tggtagacgc accaccttga
     4321 ggtggtggcg ccggaaacgg tgtgctggtt cgaatccagt ctcgggcaca aagcgaagca
     4381 cttgcttcgc tttttttatg ataaaggtat cgtcttaatc catttcctcg ttttgtttaa
     4441 ggctgctttt atcagcatcc ttttcttttc atcaatatac ttttccagtt ctttcagctt
     4501 gttatactcc tcaaaagatt tgggcaggta atctttcctg aaatgcttta ttctttgcaa
     4561 cgcaatttct ttatcctgcc agctacccag gtcatcaaga atattatgta ataattccgg
     4621 gtgatattca ctattgtcat ttactatgtt gatccagtag taaactgatt tcaggttttt
     4681 gcgaagcagg tgaggttttt tgaattgtga ttttgtctcc ccggcagagt gcctgataat
     4741 agattgtata ttttggttca actccccggt attttcgatt tcattgtctt gtttcaatac
     4801 gaatgcaatt ttttttgtct ctttattctt gtaacgatga atttcctggt tggaccatgc
     4861 ttttgtaatt tttaaaacgg cttgcaaata aagagtgaat tgcgggtagg aataatttat
     4921 ttcttcttta aaagacgtca atacctccat gcagatttct acatcccgtt gccggccaat
     4981 agtatcaaac agttcttctg ttttatccag taaccctggc agtccggctt caaccggatc
     5041 agtcactttt tttaattggg aatatagttc aaggtaagcc cggagttttt taatagccac
     5101 tctgaggtca tgaatagctt ccttatccag tttttttcgg agcaaagaaa gattctcgtc
     5161 aaatactttt tgctcttttc gccaattgat taatatcggg tttgcgttgt tcatatgcac
     5221 aaaccggccc ggcaacatgc cctcgccttt gcctgctaaa taacgataat cccgcaggtt
     5281 tatctatgag ccgcgtcagc cggctctatt atttttattg accgaaagta taagtgaact
     5341 caattttccc ggtgatctgc ttgtcgttgt tgaaaagcac ttccttggtt ttaagacctt
     5401 tttcatcata gatatagcgc cagatcaggt agccaaggtg aaggctggaa gtagtggtcg
     5461 ttttctgggc tatattatct ttttcatcat actcaaacat gatgtccggc atcagtttcc
     5521 tgagcctggt attgtagcgg acaatatcca gtaaacgatt cctgtcatca taatagtaat
     5581 agaggtagct ggtttccacc ccttttttgt aggttttttc ttcagctgta tttccatctt
     5641 cgtcagttat aaaacgtact tcaagactgt caatggtatt ttcagctcct gtattattca
     5701 ggatacgcca catcttttca ggttttccgg atgccgagta aaaccattga tgtgtttcgg
     5761 aatgatcaaa gtcactggca gaatctttta cgacattcag tatctggcta actcttccag
     5821 ttgcatcata ggtataagtc gtgctgctct gaatagctgt tgatgaatca gtaatattta
     5881 tcacacggcc cttttcgtca aacctggaat aaacaactgt tttattgaaa ttgttaatag
     5941 atgagttttt taggaccctg ccgttttcct ttacttcctg gaattctgaa aaatcggttg
     6001 ctttcacacc ccggttatca tacccggtgg cactaaccgt ttttacttta ttcgctacat
     6061 agcttttcat caggttgtta gtttcctgtg tgccaattat atcgttatag taatactgtg
     6121 cctgcagggc caaagaggtt aggagagtaa cgggtaatag aaggattttc atgtcgtgat
     6181 atggttttta ctgttcaaag ttaccttaat tgttcagtaa cgaaaaagaa tggttccctg
     6241 tattttttgt acttattttt aagaaaaatt tacagtgtct cacattcctc agcgaaaaga
     6301 taaagactgc ctgaactgtg gaacaaccgt acaagggaat tattgtcaga attgcggcca
     6361 ggaaaatgtg gttccgcatg aaacattctg gcatatggta aagcatttct tatatgatat
     6421 cacccatttc gacagtaagt tttttgattc gatgaaattc ctgctgctgc ggcctggttt
     6481 tttaccgcag gaatatataa agggaaggag agccagctac ctgaatccgg tcaaaaaata
     6541 tgtttttact tctgctgtat tctttttgat cttttttggt ttttttaaag gcggtgatgt
     6601 ggttaaaata aatctcgcta ctgaactgac aagagagcaa agggctaact atttaaaacg
     6661 tggagagcag gaattgttga aagatactgc aagtgagatg tggaaggagg cgctgcaagt
     6721 gttaaaggat acctctaaag tactgaccgg ggaggatatg ataaaatatt gggatgatat
     6781 tgagtttatc aacctgagtg gtaaaaaata taccaacctg aaagagtatg attcaataca
     6841 aaaagcgatt cctgctgtgg aaagggatgg ctggctgcaa agaactattc agcgaaagaa
     6901 cctgaagtta aaagagaagt accggagcga cccggggaaa ggatttaata cattgggaaa
     6961 tacttttttg cacaaacttc cttacctgtt atttgcttca cttcctttgt ttgcactgat
     7021 cttaaaattg ctgtatatcc gccgaaaaaa tttttactat gctgatcatg ccatttttag
     7081 tatatatcac tatattttca gctttatcct tttattattc gtttttggtt tggataaact
     7141 cagcacagta acgggcctgg aagtttttga tgcacttacc ggcttgctat tcctttcagg
     7201 gggagtatat ttatatatct cgatgaagcg attttatggc caaaggcata tgaaaacctt
     7261 ttttaaattc ttattattga atgtatccgg aattattatg ctggttgtat tatttgctgc
     7321 ctttatactg ttttcagttt tcgaaatata actatactta tgccgacaaa aaatgaaaat
     7381 gcatttacga ggttagttga aataatggat gacctgcggg aaaaatgtcc ttgggataaa
     7441 aaacaaacta ttcaatcact ccggcaaatg accattgagg aaacatatga actggctgat
     7501 gccattacgg aggaagattg gaagggagta aaagaagaat tgggagattt gttacttcat
     7561 attgtttttt atgcaaaaat cgggaccgaa caacaacaat ttgaacttca tgaagtgatt
     7621 actggtattt gtgaaaaact ggttgcaagg catccgcata tatatggcga tgtaaaagtg
     7681 gaaaatgagg aggatgtaaa aagaaactgg gaaaagctta aattaaagga gggtaagaaa
     7741 tcagttctaa gcggggtgcc gcaatcatta ccagcaacag taaaggccat gcgtttgcag
     7801 gagaaggcca aacaggtggg ttttgaatgg gataataaag agcaggtttg ggaaaaagtt
     7861 gaagaggagt tgcaggagct caaatctgcc gtagtgagtc gtgaggcgaa taaaatgggc
     7921 ggggatattg aaaaactcca aaaggatgtt gaggatgaat tgggagatgt actattctct
     7981 cttataaact atgcacggtt tttgcaggta gatgccgaaa atgcactgga aagaacaaac
     8041 aagaaattca tctaccggtt tacacaaatg gaacaacaag ctattcaaca agggaaaaat
     8101 ctgaatgaca tgaccctgac tgaaatggat gccatctgga acaccatcaa acaacagcgg
     8161 cccaataagt gaagccacaa ctaaaaaata tattttggag taagttcagt ttcctagtac
     8221 tcgcagtcct gttctttacc ctttcttttg tttttaataa actttattcc agccgcactt
     8281 ctgttacaag ggaggcagaa cttgcagaaa catttatccg caatcaacag aaggatttta
     8341 ccgacctgtt aactgatacc gccttaatca gaaggttgct caataaaaaa gaatctttaa
     8401 gtgagctgac taatttgtcg gccagaaact atggtatctt tctatactcc gtaaatgagt
     8461 tctatgatgt gaccatgaaa ttctggagtg atcagttgat tgtcccccct ccggaaatgc
     8521 tttcagcaga agacggagaa tattttaccc agttatccaa tgggtattat tatataatta
     8581 agaaaagcct cgggtcagat ggtaattcgg ccggaacgct ggcctttgca atgatcccgg
     8641 tctggtctga gttttttatt gaaacggatt atcttccaca gaaatttact tatagtaata
     8701 cagccgccag atgggtacgt atcagtgacg ctgttaccga gtttccggtg aaaacttttt
     8761 caggtaaaac actattttac ctggataaga aagtttcagg ggccgtaccg tttaatgatt
     8821 tattgaccct tttcctgcgt tttggggcat tgatattttt attccttttt attcacctgg
     8881 cagcagagtc cacttcaaaa aaatatggcc cgtggaaatc aattgcttta ttggtctttt
     8941 ttttactaag tctccggctt gtctcctatt tttttccttc cttattaaac ctgcgtcagt
     9001 ttgaaatatt taacccggtt atttatggtg cagatgcggt tcagcgttcg ttgggagatc
     9061 tgcttattaa tgcgttactg ttatgctggg ttgtattatt tgcatggtcg aagctccggt
     9121 atattgagaa tccggtatca gggttatcgt taaaattgcg ttggttattg ggtttattgg
     9181 ccctgtgtac gctcatttac tcatcatttg tgctggctaa tgtggtacgg agtcttgtct
     9241 ctgattcaaa aatatcgttt gacgtcacta actttttcag cctggactgg tatacagtag
     9301 ccggttttgt tgtactggcc tgtctttcat tgtcctatta ttatctttcg cagttattgt
     9361 tccggtttat tttccctttg tttgcgggaa gaaattattt tatttatttc tcaattgggt
     9421 ttaccggatt agtttacctg accttccgtt cgggcaatcc tgaagtgctt ttttatctgc
     9481 ccgtattact atggttgctt atttacactt ggctgataca tcgccagggt atcatcttta
     9541 aacgtattcg tatcaatatc gggggaatgc tttcctggtt atttattttc tcggtttctg
     9601 ttgccgttat catgctctca gagaataaga aagtggagtg ggaaaaaaga aagcggatgg
     9661 cagatgtact ttctgtgcaa acggataaat caagcgagat gctgatgagt atagctattc
     9721 agtatcttga taatgatttt ctccgcgaga atttcaatcg tttcagtgat cccgaaaatg
     9781 gcaaagagtt ccgggacagt atcgttacag ataattacag gggttatctt aataaatatg
     9841 atacccggct ttatgtatat gatgaatata acaggcccct ctataatgaa gatgcaactt
     9901 cgtatgagtc attgaataca attctttccg tacaatcaaa gcctacacaa attgagggat
     9961 tgttttatta tgagacttct tatgacaagt ttaattatat aacccggcgg gaggtaaggg
    10021 atacggcaaa taaaaagatc ggttctttct ttattgtttc taatcctaaa aacttcagta
    10081 atgaagcatt gtttccggag cttttcaggc agttcaaaaa atcagatccg gagagttcgc
    10141 ccatttattc tattgcagtt tatgctggtc gccgccttat gtctccaccg ggtaattatc
    10201 cttttcctat atggcttatc gatgtggaag ttccaaagga ggagtttctg caaagaacaa
    10261 acggagattt tgatgaactc tggcatagat cgggtaatga taaagtggta gtaatagccc
    10321 ggaaaaagga gacttttatc caggccatta ctttgttctc ttatattttc tgttctttcc
    10381 tgtttatggt ggcttttgta aaactggtat cacttgtttt gaaaattgga accaattggg
    10441 caggtatccg caggctattt caatttaata tccggagaca ggtacatagt actatcatat
    10501 tcatcagtat tttttctttt ttggtcattg gggctgctac tatctccttc ttcattaacc
    10561 gctataaaga aaataacagt gagaaactaa gccggacgat gaagataatg gtgaatgaaa
    10621 tggaaaagaa aatggctgat cataatacat ttgatgatgt gatcaaaatt tatgactcgg
    10681 tctcaaatac cggtctgcaa ggacttgtgg atgaagtgtc agaaattcat ggagtagatg
    10741 tgaatgtata tgacctgtca ggaaatctgc aggtttcttc agaggcgaat gtgtatacca
    10801 aaggggtgtt gagtaaaaag atagatccag ttgcctatta ctaccttgac aaacttcgta
    10861 aagtgcagca cctgcagact gaaagggttg gtaatttctc atattacagt atgtatagcc
    10921 cggttcgtga tgcagatggc caggcatatg cctatatcaa tatcccgtat tttacttcac
    10981 aacctgaact ggaaaaggag atatctaatt ttttagtaac catcatcaac ctgaatgctt
    11041 ttatcttcct gattgcaggt ttaattgcaa tgtttataac aaaccggatc accgattctt
    11101 tttcattgat cagtgataaa atgaaggaag tgaatcttgg taaggtgaat gaaaaaatca
    11161 gctggagccg taatgacgaa attggcgaac tggtgcatga gtataataaa atggttgcta
    11221 aactgggtga aagtgccgca gccctggcta aaagtgaaag ggaaggagcc tggcgtgaaa
    11281 tggccaggca ggtggcacat gaaattaaaa acccgctgac gccgatgaag cttagtatcc
    11341 agtacctcca aaaagcaata aataataacc agcctaatgt aaaggaatta tctgccaacg
    11401 ttgcaaatac cctggtagag cagatagatc atttatcgaa aattgcagca gatttctcgc
    11461 agttcgccaa tattggcaat actaatgtaa gtacattcga tctgcatgac gttatcggtt
    11521 ccctgaggga attgtacgga tcagatgaaa acgtaagatt tgtatggaga ccacttaaag
    11581 acaaggtatt tgttcaggca gataagaccc agatgaaccg tctttttact aacctgtttg
    11641 caaacgccgt ggaagcctgc aatggaaatt cattttgtct tattgaagta aacgaaagca
    11701 ggcaggagaa tatattgcgt atcagtataa aggataatgg tgaaggtatt gatcctgaaa
    11761 tgcaggaaag aatattcgtt cccaacttta ctaccaaatc atctggcaca gggttgggtc
    11821 tggctatgtg caaaggaata gtggaacaag cgaagggaaa tatctggttt gaaacatcgc
    11881 agggaaaggg gactacgttt catgtcgaat taccaataat ggataacctg taataaaatt
    11941 tagtattgct gatttgcagg gcttttttac cttttgtgtt attctaaatt ccttatttaa
    12001 gattgtattt ttctgatcaa ggtactttaa aatagttgtt atgaaaaagc aaagcctgtt
    12061 agtcgtcctg tcattttttt tactttctgt aaatgcccag gtttataaaa caaatggacc
    12121 gctggtgcat acctattcta tagtagcaag ggattctgtt acaggagaga tggcagtggc
    12181 agtgcagagt cattggtttt ctgtaggaac ggctgtaagt tgggcggagg caggggttgg
    12241 ggctgtagct acccagtctt ttgtaaacaa atcattcgga ccaagaggac ttgcgttagt
    12301 taaaagtggt ctgactgccc aacaggcatt agattcttta ttggcagatg atgagggcag
    12361 agaagtcagg caggtcgcta ttgttgataa taaaggtaat gtagcaaccc atacaggcag
    12421 tggttgtata caatatgctt cacatgtaaa gggaagtcag ttttctgtac agtcgaatat
    12481 gatgctggga aataaagtgc cggttgctat gaaagaagca tttgagaaaa gtactggtaa
    12541 gccattgggt gaaagaatgc ttttagcatt ggaagcagcg caaggggccg gaggggatat
    12601 ccgtggtcag cagtcggctg cgttattgat tgttcccggc agttcggctg gcaggccctg
    12661 ggatgaaaga acagtggatt taagagtcga tgatcataaa gagccagtga aagaacttcg
    12721 acggttatat actatcagta ttgcttatga gcaaatgaat aaaggagacc tggctgtgga
    12781 aaagaatgac atggcagttg cgatgaatga atataatgca gccatgaaga tgatgcccgg
    12841 caacctggaa atgcaatact ggacagccgt tactcttgcc aataataagg agttaaacaa
    12901 atcattgtcc atattcagaa aagtatttgc taaagataag aactggaaag agctgacacg
    12961 acgtttgccg gcagtaaaac ttcttacggt aagtgaagaa gcattaaaag ccattcttgc
    13021 tttgtaattg caaacaggtg ttcctgtatt gattgcccgg gaaatagaaa gaagtaggat
    13081 tgctgcttta attactaagt aaatattgac aatgagaaat agatattcac atttctactg
    13141 ttattacccc tgttagctgt acatggcatt ttatttgcac ctatctgtaa cgcaccataa
    13201 atcctttaag ggattcacca gcccaggtta agacacagat aatatgaaaa accaaacgaa
    13261 gtttgttgtt tcacccaaca tggtgccctc taatgcttcc ataacagtac agtcgccggt
    13321 aaacatgttt gccgctgata gttatcatat cgtcaatgac aggggcgaac ttgtcaggaa
    13381 aggtaaaata tcagatcctg caagagagtt ttcacttagt gtcgggggta tgccggatgg
    13441 catatactgg ctggtgatgg gagagttgcg ggaacgattt acgattgttt aagcaggata
    13501 atcaatccca tacatatttc cagcttccat cagcctgctt tctccaaacg gtgtgaaaaa
    13561 caccccgggc ttcattggta ttgccagtgc tgtctttgga ttgccacaca tattttccat
    13621 atgtatatcc catatcacca ttatcggctg catctgtaaa gtcgggtgac catttgacgg
    13681 aagcagattt aaaatattca gctgaataat actttttgat attctcttta cccgttataa
    13741 gggtatcgtt ttctcgtttt attactgcaa ttgaatccgc aaaaaaccaa aatgcttctg
    13801 ctatgccttt ttcagcagcc attttttcaa actctttttc ggccatgttg atttcctgtt
    13861 tggctttttc cttctggtct gtaactccag gttgacagga aacagccgat agggaaatgg
    13921 taataacagc aatagaaaat tttcgtatat gcatgatttc tattgttaat aatttaactg
    13981 accgttggag taatattgat tggatgcccc gtgaagttta ccaccccata atgtcagcca
    14041 tacctttaat agtgaatata acacctatag tagtgaggcc gtacatggcc catttgatat
    14101 ctcctccgct gctgtaaagc aagatagtga gcatgcaacc tacaaccagt aatgcgacgc
    14161 caataccaca gcaggcaaaa ccagtatttc tttttttgat gagccgtaaa ttttttagct
    14221 gctgaatggt ttcctgcagg agattttccg tagctccctt ctctttgagt tgcagggtta
    14281 tttcgtcata actcaatcct ctttgctgaa gggaattggc taaatgcaaa aattcactgt
    14341 gctgaacagt attcataacg caagctttgt gttaaaatac aatttttttt gaaacgacca
    14401 aaaaaggctg ctactgtgta cactcaacca atggcacgcc ttcatataca gcatattgag
    14461 caatgctatg ctggtaaccc gcttcgccca accagttcag aatatcattc cccgtattct
    14521 taccggagag tggaatgttt acctgtttac cgtttccatt ggtttgtaca tatggggaaa
    14581 taccactggt aaattcaagc agtaccatag ccgccttcag catagtgaat acggtactga
    14641 gtgaaacagt caggttctta tagccaactt ctattttgcc ggcagataat gagtcattat
    14701 ataaactacc caacgaagtg atcttagctg cactgggaaa ctttggtaca atatcatttt
    14761 tgttttctac ccggatggag gctggaaatt ttttattaaa atcattggca aatgcctcat
    14821 tgcctgtagc gggtgccgca aaagtgatga cattaatatt gggaaccaga tgttgtttag
    14881 ttttaaatat ttgccacagg taagaggcat atgtgacagc caggttgcca cccaggctat
    14941 gaccggttat cagcagggga gcgttattct tcaatacagt ttccagcaag ggcaatagta
    15001 actgacccgt tttcttatca gtcatcctgc acaggttttg ccagccatca aaagcacctt
    15061 gtgccagttt tgcattggag gaatcattgg tgaaagtcca tttttcctgt gaggccacat
    15121 gcaggtcctg gtagatccag ttttgaaaag ctgcccagct gaaattaatc aatgaacccc
    15181 ttatggccaa tgcaaattct cttccattgc tggcaacaaa tgcatgatta cctcctaact
    15241 cagctgcctc ccaaatgggc ttccaacccg gcatatactt attaatggca gagtctatat
    15301 ttgtacaata ggcaatctgt gaaagcaggc aagccgtttt ttctgttaat tcagaaatta
    15361 ttaccgcttc cgggttttct ttttctttct tattttcttt ctgtttacag gacagagtga
    15421 gtaataaaaa gctgagaaag aaataaagac ttactctcat gattggtaat tattttatac
    15481 taaaaatatg catttatgaa tttctatata gatgtttgtg caatggcagg caatcaacct
    15541 gacgcatata tgtttcaaat ccgtattatc aggaaaacta ataacttcac tttctgaaat
    15601 atcaataata ccagtatgaa aaaagttttc cttatac
//
LOCUS       DAORTI010000082        15610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3541_length_15610_cov_6.655673, whole genome shotgun sequence.
ACCESSION   DAORTI010000082 DAORTI010000000
VERSION     DAORTI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15610
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3541_length_15610_cov_6.655673"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1702
                     /locus_tag="PLO70_10915"
     CDS             83..1702
                     /locus_tag="PLO70_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQZ75021.1"
                     /translation="MKKIFTIIILLSTIIPAPAQQEVKRPKLVVGLVMDQMRWDYLYR
                     YYNRYSETGGFKRMLNEGFACENTYIPYAPTVTACGHSSIYTGSVPAINGITGNYWWD
                     NEQMRSVYCTEDKTVNTVGSNSSQGKMSPRNMLVTTICDELRLATNFKSKVIGVAIKD
                     RGGILTAGHSANAAYWYDNSVGNWITSTYYMNELPKWAADFNNQKIVDQYYEKGWSLL
                     YPENSYLQSTSDEKAYESKPFGTKFPYTMKNFAGKDYGKITSTPMGNSITIEFAKAAL
                     TAEQLGADNITDFLAVSFSSPDYIGHTFGPNSIESEDGFLRLDKELGDFLDFLDTKVG
                     KGEYTVFLTADHGAAHVPEFMQENNLPGGRVFMSTVTGNLNKLLNEKYRINNIIVSDE
                     NYQIHLNHPALDSAGLNKSDIIDRIINSLLTVPGIDRAFALNDLNKIPLPSTIKNMLS
                     NGYYPRRNGDIQFILKPNYIDAWNNTGTTHGLWNPYDSHIPLIWYGWGIRKGKTNRDT
                     YMTDIAPTIGALLHVQMPNGNVGQVITEVLQ"
     gene            1789..2820
                     /locus_tag="PLO70_10920"
     CDS             1789..2820
                     /locus_tag="PLO70_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75022.1"
                     /translation="MKKACCFLLTCFMAIAFTAKAQVETVDSIRFFTDEGLITMSLAT
                     DIRQLQSEKKQDVFQPATVSMKFPDSTVINESIMVGARGKFRRSYCRIPPMMLNFRNN
                     SSPRLQSLGKLKMVIGCGVASSDEELLLKEFLVYKIYNLLEEKSFRVRLLKVNYSDTR
                     NKVKPFDQYAFLIEDDGDMARRNGCRKKADAKFLTEATNRDMMTMVAIFQYMIGNTDW
                     AVPNNHNTKLIYDRSNEKAPPYVVPYDFDYCGLVNAEYAIPNELIGTEKVTERVYRGF
                     PRTMEEIQITLDIFRAKKDSIIALINNFQLLSNRTKKEMLYYLEDFFRIINNKNEVKS
                     IFIDNARTS"
     gene            2889..3146
                     /locus_tag="PLO70_10925"
     CDS             2889..3146
                     /locus_tag="PLO70_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HQZ75023.1"
                     /translation="MELKEQFEKAIADSKSLTEKPSNDTLLQIYSLYKQATDGDINIE
                     PPSNPFDFVAKAKYEAWAALKGKSTANAMTDYVELVKKLKG"
     gene            complement(3160..4323)
                     /locus_tag="PLO70_10930"
     CDS             complement(3160..4323)
                     /locus_tag="PLO70_10930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ75024.1"
                     /translation="MSRKKILWLCSWYPAKTDPYNGDFIQRHARAAALYNDIYIIHVA
                     GDETGNTKKPEEGKYQSEGLTEHIIYYQKSTSFFGRLLSHFRWLRLFKAAINNYIGNN
                     GIPDVVHVHVPVKAGLFALWFKRKYKVPFVVTEHWGIYNDVEIHNYQGKSAAFRQLTR
                     RVFEKADVFLSVSQFLAEGVNKLVVEKKYEIIPNVTDTSVFYYKDRKNVVFRFIHVSN
                     MVPLKNAEGILMAFRLLLQQITNVELVMVGDTNTSIRNFAKELGLTGSQVLFRGEIPY
                     YEVAREIQEADCLVLFSNIENSPCVIGEALCCGLPVIATKVGGIPELVNEENSILIEP
                     KDEKALVAAMQQMITRFSEFNHKKIAEKAQSRFSYPVAGKKFNEIYSLLAARN"
     gene            complement(4342..4734)
                     /locus_tag="PLO70_10935"
     CDS             complement(4342..4734)
                     /locus_tag="PLO70_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75025.1"
                     /translation="MQRKNQVLLSFLKLRAIVNGKEIYPLQNSKPVVIPVQANNPRIV
                     ITDGYHITKPLKLIYKDIHTYCFKVVCAISDQQLLGGAVVLAGLYLSGFYTGLLVLKV
                     FSFLPVIYLLMFYYLNRKEFIRLVPVLN"
     gene            complement(4835..5596)
                     /gene="tpiA"
                     /locus_tag="PLO70_10940"
     CDS             complement(4835..5596)
                     /gene="tpiA"
                     /locus_tag="PLO70_10940"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQZ75026.1"
                     /translation="MRKQIAAANWKMNLTYQNGEKLLDDILDANIKLSSHQLAIFAVP
                     YPYLIMTRSEVEDEKNYYAAAQNCHHKRSGAYTGEVSADMLHSIGINYCVVGHSERRE
                     YNGETNAMLADKVNLCYENNIIPVFCCGEPLAIREEGTQNSYVAAQLRESLFHLPAEK
                     IKEIIIAYEPIWAIGTGKTATTEQAQEIHHFLRTVIADQYSESVADDIPILYGGSVKA
                     NNAKELFSCPDVDGGLVGGASLVAEDFIEIIKALK"
     gene            complement(5605..6783)
                     /locus_tag="PLO70_10945"
     CDS             complement(5605..6783)
                     /locus_tag="PLO70_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HQZ75027.1"
                     /translation="MNKIIFTEEFCQPENLHPFTLTRQIQDIRIGILTIREKWELMLG
                     LISYDKKEDDYKDLDRSVNIDEVIGDDVCFMIHGNVLPTSKLVKAILRLKEGEFIATP
                     GGNSVAFCITKNEIADKYKIKVGHAVTYREEIKTIQFPWDIFHLNDWAIRQDFELLTS
                     KRKSQPISKTNKVIKPSQLFIEKGAKVEHCIINASTGPVYIGKDAEVMEGCVIRGPFS
                     LGEKACLKMGTKVYGATTVGPQSVVGGEIKNSVLFGYSNKAHEGYLGDSVIGEWCNMG
                     AGTTNSNLKNNASDVKVWTPKGELNAGPKCGVMMGDYSRTAVNTAINTGTVIGVSCNV
                     FGSGLTPKYIPNFSWGSEGVKRYEFEKALLDIEDWKKLKGQIISDDEKSILKYIFEKF
                     "
     gene            complement(6899..7153)
                     /locus_tag="PLO70_10950"
     CDS             complement(6899..7153)
                     /locus_tag="PLO70_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HQZ75028.1"
                     /translation="MKKGIHPDSYRLVVFKDMSNGHTFLSRSTAASKESVKWEDGNEY
                     PLIKLEISNTSHPFFTGKNMLVDTAGRIDKFKKKYAKKAE"
     gene            complement(7583..8074)
                     /locus_tag="PLO70_10955"
     CDS             complement(7583..8074)
                     /locus_tag="PLO70_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQZ75029.1"
                     /translation="MPSFDIVSKVEAQALDNAVNVTNKEITNRFDFKGSHVVINLDKK
                     DFLIKLETDDDMKMGQLIDVLINRAHKQGIAPEAFDASKQGAQSGKTWKKEVKVRNGL
                     AQEDAKKIVKLIKDAGLKVQASINDDLVRVTGKKIDDLQSVIQLCKTSNLNIPLQYVN
                     MRD"
     gene            complement(8175..10643)
                     /locus_tag="PLO70_10960"
     CDS             complement(8175..10643)
                     /locus_tag="PLO70_10960"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75030.1"
                     /translation="MRKYLTFCYLLFVCTTGVFAQQKTGKVSGQVQDINNKPLSAVSV
                     SLLKTVDSALVKTAVTGKDGKYEFENIADGEYLILTSAVGFEKIYSNSFSIAATSNSV
                     EVAALKMSVASTSLEGVTITAKKPFIETKIDKTVVNVDASPTNAGATALEVLEKSPGI
                     MVNNDGIISLRGKAGVIVMMDGKPTFLSPSDLANLLKNMPASAIDQIEIMTNPSAKYD
                     ASGNSGVINIKTKKGRAAGLNGSVMVGLTTSYFEQNGTLYVIPKSQNSINFNYRKNKI
                     NFFGNYNPNVFSGRSSLDITRTKLDANKNVLGYNDVETSFKFGNNNHTLKLGLDFFAD
                     KKNTFGIVVSGFTFSGHPTPGTITTTSDENYQPISIMVSQTDNKLRFQNFTSNFNYRH
                     QFDSTGRELTADLDFIAYDNTSRMILTTDFFDGSHQPAGDQLVLKGYLPSSIKIYSAK
                     SDYVHPFKKGGRLEAGIKSSYVTNDNLVNYERWDVSKWIADGRSNHFIYDENINAVYL
                     NANKKMKKWSFQGGLRLENTVAKGYQVTNDSSFKRNFTNLFPSAFVSYAANKDNQLTV
                     SYSRRITRPNYQDLNPFIYFLDSLSYRKGNPFLLPQFTHNIELSHSFKGKLITTLNYN
                     TTNDVISQILKADGDLVFLTSDNVARFRNIGIAITAPIPVTKWWTSNIFTNIYNNHYE
                     GMYEGKPLDISYTSFMINMTQTFTLKQGFTLEMTGFYRAKGVDQLSINEPMYVISFGG
                     QKQVLKGKGTLRINLRDPFWLQRYKGSTQYDIIDTRVQSRWDNRQVTASFTYRFGKNG
                     QQNSPRRRNSASQDEQNRVGQGGQ"
     gene            complement(10898..13360)
                     /locus_tag="PLO70_10965"
     CDS             complement(10898..13360)
                     /locus_tag="PLO70_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75031.1"
                     /translation="MRKLLTLLTVGLTALSFTSQAQFTGGKVKGTVIDGSAKTIESAT
                     ITLLRVKDSSVAKITAADKTGKFEFDVVPEGTYMVSVSAVGHSKGYSAVFEINPVSTL
                     VTLKTIELTPQAKSLGGVTVTAKKPLIEQKIDRTIVNVEASVTNVGTSAMEVLEKSPG
                     ISVDKDGNISLKGKQGVQVYIDGRPTYLSGTDLANYLRSLSSNQLDQIEIMTNPPAKY
                     DAAGNSGVINIKTKKTKQFGYNGNLSSTYSQGRYAKLNESFSFNYRKNKVNFFSTLGY
                     NSRKNFQDLNIQRKFIESSTKEVKSNFEQLSRIRDNGKSHNAKLGLDYFASNKTTLGV
                     VFSGFYNPGKFGNHSDVMIYDPAMSLLSQTLASTSNDRKWKGFSTNLNLRHVFDSTGR
                     ELTVDVDYLTYRSKNSQDLINAYYDAHGLPTIKADTLLGSLPQDINIYTAKMDYTHPL
                     NKGAKFEAGVKTSFVNTDNNAIYDSINYGVRVRDIGRTNHFIYDENVNAAYVNYSRPL
                     TKKLFGQFGVRLENTNANGNQVTTGEKFKRNYTQLFPTAFLQYKASEKHSLVLNYGRR
                     INRPNYEDLNPFIIFLDRYTFEQGNPNLKPQFSHNIELTHTFKGFLNTTLNYTRTTDI
                     INDVLEQDPDKNETVIRNQNIAKQRQYGIAVSAGGQITKWWSGNIYANVYNNRFEGII
                     NGDQVDIKATTGQFNASNQFKFSKTWSAELSGFYRTGGVDGVFRINGFGMMNMGISKQ
                     ILKGKGSLRFNIRDVLYSQKINGSIKYGNVDAAFQQQRDSRQAAIGFTYRFSKGKING
                     NGQKKKTGGASEEQNRVKTGGE"
     gene            complement(13676..14440)
                     /locus_tag="PLO70_10970"
     CDS             complement(13676..14440)
                     /locus_tag="PLO70_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ75032.1"
                     /translation="MSQVFKAIVIDDEPAARRIMKSLLSAYADTVSVIEEAGNGREAI
                     EKIEALKPDLIFLDIQMPDLTGFEVIERLTHKPNIIFTTAYEQYAIKAFETFSIDYLL
                     KPVREERLAQSIQKLKSFGRISNTIDVSGLQEIIRQFQAPQKATALPIKTGDRIILLR
                     YESIAYMEAQDKYVYIFTVDGTKYLTDNSLAALSEKLPQQFYRIQKSYIINKDRIREM
                     HRHFNGRYLFVMDDKVGSRLTSGRTYHDLIRSDFGL"
     gene            complement(14424..>15610)
                     /locus_tag="PLO70_10975"
     CDS             complement(14424..>15610)
                     /locus_tag="PLO70_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ75033.1"
                     /translation="LILAFCMKEPTTLEYIILIFSCLLFALAGLYILTNFTRLFKQSV
                     RNELLRKNIVAWLAIFCISTAMLPMSGGFFYMLAKWGWWWSVIGQFISTTATTLVVYN
                     TVRFIAENKRFKKLTFIQHNALIVFSLIVTTVAINIPFGYILYGSETSYLKYIIINSI
                     YLAGATGLIYAVISYLDIERKRKFDAKELELSRMSELKAKAELDALHSKVNPHFLYNA
                     LNSIADLSMTDGKKARKMTIALADLFRYSINYSQNNYSTINDEMAMTEVYMQIEKIRF
                     EDQLTYSVYVEPATAHYQIPRFILQPLAENAVKHGLKATGKLTEIFLEVRRKDNGVII
                     NIADNGPPFPDELVPGYGVKSVFDKLDLLFPGQYEIHFSNVPRKQVSIQINKLLKNEP
                     GL"
BASE COUNT         4615 a         3260 c         2881 g         4854 t
ORIGIN      
        1 tttttaagcc aggtttatcg tcgtaaaaag gtgacaattc caaaacctgt aaaaccctac
       61 atttgcctaa attaaaaatt gaatgaaaaa aattttcaca ataatcatac tgctgtcaac
      121 tattattcca gctccggcac agcaggaagt aaaacggcct aaattggtcg taggattggt
      181 aatggaccag atgcgctggg attatctcta tcgctattac aaccgatata gcgaaaccgg
      241 cggtttcaaa agaatgctaa atgaaggctt tgcctgtgag aatacctata ttccatatgc
      301 acctactgtt accgcttgcg gccatagttc gatttatact ggttcggtac ccgcaattaa
      361 tggtatcacc ggaaactatt ggtgggacaa tgaacaaatg agaagtgttt attgcacaga
      421 agacaaaacg gtgaatacgg tcggcagcaa ttcttcacag ggaaagatga gcccccggaa
      481 tatgctggtc acgacaatat gtgatgagct acggctggcg actaatttta aaagtaaagt
      541 gataggtgtc gctattaaag accggggcgg tattttaact gctggccata gtgccaacgc
      601 cgcttactgg tatgataata gcgttggaaa ctggattact tccacctact atatgaatga
      661 attaccaaaa tgggcagccg attttaataa ccagaaaata gttgatcagt actatgaaaa
      721 aggatggagc ctgctgtatc ctgaaaattc ttacctgcaa agcaccagcg atgaaaaagc
      781 ttatgaatcc aaaccatttg gtacaaagtt tccctataca atgaaaaact ttgccgggaa
      841 agattatgga aagatcacct cgacccctat gggaaactca ataactattg aatttgcaaa
      901 ggccgcactc acagcagaac aacttggggc agataatata acagacttcc tagccgtaag
      961 cttctcatca cctgattata tcggccatac attcggcccc aactcaattg aatctgaaga
     1021 tgggtttctc aggctagata aggaacttgg cgattttctt gattttcttg atacgaaagt
     1081 gggtaaaggc gaatacacag tttttcttac tgccgaccat ggcgcagcac atgttccgga
     1141 gttcatgcag gaaaataatt tacccggggg cagggtgttt atgagtacag tgaccgggaa
     1201 ccttaataaa ttactcaacg aaaaatacag gatcaataat atcattgtaa gtgatgaaaa
     1261 ttatcagatt catctcaatc atccggctct tgactctgca gggctgaata aatctgatat
     1321 aattgatcgg attatcaata gcctgttgac cgttccgggt atagacaggg catttgcctt
     1381 aaatgacctg aataaaattc cattgccttc cacaataaaa aacatgctga gcaatggtta
     1441 ttatccccgc cggaacggag atatacagtt catcctgaaa cctaactata tagatgcctg
     1501 gaacaataca ggtactacgc atggattatg gaacccctat gattctcata tcccattgat
     1561 ctggtatggc tggggtatcc ggaagggaaa gaccaaccgg gatacttata tgacagatat
     1621 agcaccaacc atcggcgctt tgctgcatgt gcaaatgcct aacggtaatg tgggacaggt
     1681 gataactgag gttttgcaat aatattagag ggtattgtga aatatggttg tattttcttt
     1741 tgtaacttcc tgatgcaaaa cctacattca ccactaatta ttaccaaaat gaaaaaagcc
     1801 tgttgttttt tacttacctg ctttatggca attgccttta cggccaaagc tcaggttgaa
     1861 acagttgaca gcatccggtt ctttacggat gaaggcttga ttactatgag cctggcaacg
     1921 gatatccgtc agttgcagtc agaaaagaaa caggatgtat ttcaacctgc cacagtatca
     1981 atgaagttcc ctgatagtac agtcataaat gaatccatta tggtaggtgc ccgggggaaa
     2041 ttcagaagaa gctactgccg tatcccgccc atgatgctca atttcagaaa taacagttct
     2101 ccccgtcttc aatcgcttgg caaattaaaa atggtgattg gttgtggtgt tgcttcatct
     2161 gatgaagagt tattactgaa agagttcctg gtatataaaa tctataatct gctggaagaa
     2221 aaaagttttc gtgtacggtt actcaaagtc aattattccg acacgagaaa taaagtaaaa
     2281 ccctttgatc aatacgcttt tcttatagaa gatgacggag atatggcccg cagaaatggc
     2341 tgccgaaaaa aagccgacgc taaatttttg actgaagcca caaaccggga tatgatgacc
     2401 atggtagcta tttttcagta tatgatcggg aatactgatt gggctgttcc taacaatcac
     2461 aatacaaaac taatttacga caggagcaat gagaaagctc ctccttatgt agtaccatat
     2521 gactttgatt attgtggcct ggtaaatgca gaatatgcta tccccaatga attaataggc
     2581 actgaaaaag ttactgaaag agtgtacagg ggatttcccc ggacaatgga agaaatacaa
     2641 ataacgcttg atattttcag agcaaagaaa gatagcatta tagcactgat taataacttc
     2701 cagcttttga gcaaccgaac caaaaaggaa atgttgtact acctggaaga ttttttcaga
     2761 attatcaata ataaaaatga agtgaagtca atcttcatag acaatgcaag aacaagctga
     2821 tcctgacttt ttgtgaataa ccggcccctg acaccttatc tttttctcaa aataaataat
     2881 taaaccagat ggaattaaaa gaacagtttg aaaaagcaat agcagacagt aaaagcctaa
     2941 cggagaaacc gagtaatgac actttactac aaatttactc gctttataaa caagctacag
     3001 atggcgatat aaatattgag cccccatcaa atccattcga ctttgtagct aaagcgaaat
     3061 acgaagcttg ggcagcctta aaaggaaaat cgactgcaaa cgcaatgact gactatgtgg
     3121 aactggttaa aaaattaaaa ggataaagtt gttgagatat tagtttctgg cagcaagtaa
     3181 agaatatatt tcattaaatt ttttcccggc aacaggatag ctgaatctgc tttgtgcttt
     3241 ttcagctatt tttttgtgat tgaattcaga aaaacgggtt atcatttgct gcatagctgc
     3301 tacaagagct ttctcatctt ttggttctat cagtattgaa ttctcctcat ttactaattc
     3361 cggtatacca ccaactttgg tggcaataac cggtaacccg caacacaatg cttccccgat
     3421 cacacaggga gaattctcaa tattactgaa caatacaagg caatcagcct cctggatttc
     3481 ccgggcaacc tcatagtaag gtatctcacc tctgaacaaa acctgcgatc cggtcaaacc
     3541 aagttctttt gcaaaattcc tgatagaagt attggtatct cccaccatca caagttcaac
     3601 atttgtgatc tgttgcagca ataacctgaa tgccattaaa atcccctccg cattctttaa
     3661 aggaaccata ttagatacat ggataaacct aaatacaacg ttcttacggt ccttgtaata
     3721 aaaaacagaa gtatctgtta cattaggaat aatttcatat ttcttttcaa cgactagctt
     3781 attaactcct tcagcaagga actggcttac actgagaaat acatcagcct tttcaaaaac
     3841 tcttctggtc aattgcctga aagctgcaga tttcccctgg tagttatgaa tttccacatc
     3901 attatagatt ccccagtgtt ctgtcaccac aaaaggcact ttgtactttc ttttgaacca
     3961 caatgcaaat agccctgctt tcacaggcac atgcacatga accacatcag gtatcccatt
     4021 gttcccgata tagttattga tagcagcttt gaataacctt aaccagcgaa agtgcgataa
     4081 tagcctgcca aaaaaagaag ttgatttctg ataataaatt atgtgttctg tcagtccttc
     4141 agattgatat tttccctcct ccggtttttt agtgttgccg gtttcatcac ccgccacatg
     4201 aattatatag atatcgttat acaatgcagc cgcccgtgca tgacgttgga taaaatcgcc
     4261 attgtatggg tccgttttgg cggggtacca gctgcacaac caaagtatct ttttacggga
     4321 catggatata aaaatagctg attaatttag taccggcacc aggcgaataa attccttacg
     4381 attcaggtaa taaaacatca ggaggtaaat aactggcagg aaactaaata ctttcagtac
     4441 cagtaatcct gtataaaacc ccgagaggta caagcctgcc agcacaactg cacctcccag
     4501 taactgctgg tcactgatag cacataccac tttgaagcaa taggtatgaa tatccttata
     4561 aattagtttc agtggttttg taatatgata cccgtccgta atgacaatcc ttggattatt
     4621 agcctgaaca ggtatcacta ctggtttgct attctgtaat ggataaatct ctttaccatt
     4681 aacaatagcc cgtaatttca ggaaacttag cagtacctgg ttctttcgtt gcaatacgat
     4741 gtgatatgga gcggctataa ctgacaggtt aaaagttgaa agtatttccg actaatgatt
     4801 gccggctgca taaaccggac accctacaat agaatcactt cagtgccttt atgatctcaa
     4861 taaaatcttc cgccactaat gaagccccgc ctaccagccc tccatccaca tcaggacagc
     4921 tgaataattc ctttgcatta tttgctttca cactaccgcc atacagaatt ggaatatcat
     4981 ctgccactga ttcactatac tgatcagcaa tcacagtacg aagaaaatga tgtatctcct
     5041 gtgcctgttc agtagtggca gttttaccgg taccaatcgc ccagattggt tcataggcta
     5101 taataatctc tttgatcttt tccgcaggca gatgaaacaa agattctctc aattgtgcag
     5161 cgacataact attttgagta ccttcctcac ggatagcaag tggctctcca caacaaaata
     5221 ccggtattat attattttca tagcaaagat tcactttgtc tgcgagcatt gcattcgtct
     5281 ccccgttata ttctcttctt tcgctgtgac cgacaacaca atagttgatg ccaatagaat
     5341 gaagcatatc tgctgatacc tcgccagtat aagcacctga tcttttatga tgacaatttt
     5401 gagctgctgc ataataattt ttctcatctt ccacttcact tcttgtcatt atcaggtaag
     5461 gataagggac tgcaaaaatg gccaactggt gagaagacag ttttatattg gcatctaaaa
     5521 tgtcatccag taatttttct ccgttctggt atgtaagatt catcttccag ttagctgcag
     5581 cgatttgttt tctcatattc agttttaaaa tttctcaaat atatacttca aaatgctttt
     5641 ctcatcatcc gatattattt gtcctttcaa ttttttccag tcttcgatat ccagcaaagc
     5701 cttttcaaat tcatagcgtt ttacaccttc actaccccac gaaaaattgg gtatatattt
     5761 aggagtaaga cccgaaccaa atacattaca acttacgccg atcaccgttc cggtattaat
     5821 tgcagtatta acggcagtcc tggaataatc acccatcatc accccgcatt taggtcctgc
     5881 attcaactct cctttgggtg tccatacttt tacatcactg gcattattct ttaaattgga
     5941 attcgtagta ccggctccca tgttacacca ttcgccgatg actgaatccc caagataacc
     6001 ctcatgagct ttattgctgt acccaaacaa aactgagttt ttaatttctc ctccaactac
     6061 agattgcggg cctactgttg tagcaccata aactttggtc cccattttca aacaggcttt
     6121 ttctcccagg gaaaagggtc ccctgataac acatccttcc attacttcag cgtcctttcc
     6181 gatataaacc ggaccagtag aagcattaat aatacaatgc tcaactttag cacctttttc
     6241 aataaatagt tgtgatggct taattacttt attggttttt gaaatcggtt gtgatttccg
     6301 tttagaagta agtaattcaa aatcctggcg gatagcccaa tcattcaggt gaaaaatatc
     6361 ccatgggaat tgaatagttt ttatttcctc cctgtatgta accgcatggc caacttttat
     6421 tttatattta tcggcaattt catttttggt gatgcaaaag gcaacactgt tacctccggg
     6481 tgtggcaata aattcccctt ccttaagacg aaggatagct tttactaatt tagaagttgg
     6541 taaaacattg ccgtggatca tgaagcatac atcatcccct atcacttcat cgatattaac
     6601 ggaacggtcg agatctttat agtcatcttc tttcttatca tacgaaatca aaccaagcat
     6661 cagttcccat ttttccctga tagtaaggat gccgatacgg atatcctgga tctgacgggt
     6721 taaagtaaaa gggtgcaggt tttccggctg gcagaattct tcggtaaaaa ttatcttgtt
     6781 catggagttt tgaatatgtg taaaaataaa caacctcccg aagaatcggg aggtctaaag
     6841 tatagccatg aaaaacaaaa ctcctcgctt tcgctcagag tagtgtttaa agttaatatt
     6901 attctgcctt ttttgcgtac ttcttcttga atttgtcgat acggccagca gtgtcaacca
     6961 gcatgttctt gccggtaaag aaagggtgag aagtattgga aatctccagc tttatgagtg
     7021 gatactcatt cccatcttcc cattttacag actctttaga ggctgcagta gagcggctca
     7081 gaaatgtatg gccattactc atgtctttga aaactactaa ccgataacta tcgggatgga
     7141 ttcctttttt cataactcga tgattttgaa ggctgcacgg attttgccgt acagatgaat
     7201 tgataaattt ttggttggca aagataggct ggagaaggct ttcggccaaa tcttattgaa
     7261 ataaagagga aaagcctgaa aatacgaaag caccaagtga gcttactttc tggcttgcgc
     7321 cttctgttta cttacccgat gctttctgga gttactttca ccttttaagg ggcttccgtt
     7381 tctcccggga tcgatttcca ccttttgagg ggtttctttc tttcctcttg catttcgttt
     7441 tattgctaaa acgtcgtgca gttgtatttc aaatatacta tcctgaaaca cctgtttcca
     7501 aatttatgaa cactttgata tgcaccggtt ttgcactttg ttatgcacag agaaagcagc
     7561 taaaaacagg tttaccaagc tattagtccc gcatattcac atattgcaaa ggaatattaa
     7621 gattagatgt tttgcaaagc tgaattacgg attgcagatc atcaattttt ttgccggtta
     7681 cccttaccag gtcatcgtta atggaagcct gtacttttaa tccggcgtct tttataagct
     7741 ttactatctt ttttgcatct tcctgcgcca ggccgtttct tacttttact tccttcttcc
     7801 aagttttccc actttgagct ccctgctttg acgcatcaaa agcttccggg gctatgccct
     7861 gcttgtgtgc acggttgata agcacatcga tcaactgccc cattttcata tcatcatcag
     7921 tttccagttt tatcaaaaaa tcctttttat ccaggttgat gaccacatgt gatcctttaa
     7981 aatcaaagcg gttggtgatt tctttgttcg taacattcac ggcattgtct aatgcctgtg
     8041 cttctacttt acttacaata tcaaaggatg gcatagtggt atgttttgat tgaaacacaa
     8101 atataaataa gtccctcttg gaaaagaagg acctctcaca tatcaacaca ggagaaaact
     8161 gtaaaattaa ttagttactg gccaccctgc cctacccggt tttgttcgtc ctgtgaagca
     8221 ctgttgcggc gacggggtga gttttgctga ccatttttcc caaatcgata agtaaatgag
     8281 gcggtaacct ggcgattatc ccagcggctc tgtacccttg tatcaataat atcatactgt
     8341 gtacttcctt tgtaacgctg taaccaaaat gggtcacgaa gattgatacg gagtgtgccc
     8401 ttgcctttca atacctgctt ttgacctcca aaacttatta catacattgg ctcattgata
     8461 cttagctgat caacaccttt tgccctgtaa aaacccgtca tctccagtgt aaaaccctgt
     8521 ttcaatgtga aggtttgtgt catattgatc ataaaggaag tataggaaat atccaaaggc
     8581 ttgccttcat acattccttc ataatgatta ttgtagatat tggtaaaaat gttggaagtc
     8641 caccactttg ttactggtat aggtgctgta atcgcaatac ctatatttct gaatctggca
     8701 acattatcag atgttagaaa aaccaggtcc ccgtcagcct ttaaaatttg tgagataaca
     8761 tcatttgtgg tattatagtt cagggtggtt atcaatttac ctttaaaaga atggcttaat
     8821 tcaatattat gggtaaactg cgggagcagg aatggatttc ctttcctgta ggacaaagaa
     8881 tcaaggaaat aaataaaggg gttcagatcc tggtaattgg gccttgtgat cctgcggctg
     8941 taagagacag taagctgatt atccttattg gctgcatagc tgacaaaagc acttggaaat
     9001 aaattagtga aattcctttt aaaggatgag tcattggtta cctggtagcc tttagcaacg
     9061 gtattttcca gtctcaagcc tccctggaat gaccattttt tcattttttt gttggcattc
     9121 aggtaaacag catttatatt ttcatcataa ataaaatggt ttgaccttcc gtcagctatc
     9181 catttgctta catcccatct ttcataattt accaggttat cattggttac atagctgctt
     9241 ttaataccag cttctaatct tccccccttt ttaaacgggt gtacatagtc tgattttgct
     9301 gaatatatct ttatactgga tggtaaatat ccctttaata ccagctgatc tcctgccggc
     9361 tgatgagacc cgtcaaaaaa atcagtagtc agtatcatcc tggaagtatt atcataagca
     9421 ataaaatcca ggtcagcagt taattcacga ccggttgaat cgaactggtg ccgataattg
     9481 aaattagaag taaaattctg aaatctcagc ttgttatcag tctgcgaaac cattattgat
     9541 attggctgat agttctcgtc agaagtagtg gtaatggtac cgggagttgg atgacccgaa
     9601 aatgtaaacc cgctgaccac tattccaaaa gtattcttct tatccgcaaa gaaatccagg
     9661 cctagtttaa gtgtatggtt attgttacca aatttgaaac ttgtttccac atcgttatac
     9721 cccaatacat tcttattggc atctaattta gttcgggtga tgtccagtga acttcttcca
     9781 ctgaaaacat tcggattata attaccaaaa aaattgatct tattcttcct gtaattgaaa
     9841 tttatactgt tttgagattt tggaatcaca taaagtgtac cgttctgttc gaaataactt
     9901 gtcgtgaggc ccaccattac cgaaccgtta agcccggcag ctcttccttt ttttgttttg
     9961 atattaataa caccagaatt gccggatgca tcatatttag cagaaggatt ggtcatgatc
    10021 tcgatctggt caatagctga agcaggcata ttcttcagca ggttggcaag atctgaaggg
    10081 gataaaaaag ttggctttcc atccatcatc actattacac ccgccttccc ccgcaggcta
    10141 ataatcccat cattatttac cattataccg ggagactttt ccaatacttc aagagcagtt
    10201 gcccctgcat tggtaggaga agcatctaca ttcaccacgg ttttatcaat ctttgtttca
    10261 ataaatggtt ttttagcagt gatagttact ccttcaaggc tcgtagaagc cactgacatt
    10321 tttaaggcag ccacttcaac actattactg gttgctgcaa ttgaaaaact gttgctgtaa
    10381 attttttcaa acccaacagc agaagttaaa ataagatact ccccatcggc aatgttttca
    10441 aattcatact tgccgtcttt acctgttaca gccgttttta ccagtgctga atcaacggtc
    10501 tttaataacg atacagaaac agcagaaagg ggtttattgt ttatgtcctg cacctggcca
    10561 gacactttac cagttttttg ttgagcaaat acacctgtag tgcaaacaaa aaggagataa
    10621 cagaaggtta gatactttct catattcatg gcttatggtt aatgattgaa acagcaataa
    10681 aattgaagca ttagcagtta tagctgaaac gaattatgac aagtttcgta gttttcgtgt
    10741 tgaagggcaa tatattcatg cctatacaca agcggtaaaa aggctgcgtt attcaacata
    10801 aatgttaatg aagcattaac attctaaaaa aaatccctct gccaaatgac agagggaaca
    10861 cacaggtaag gtttattggt taacgttctt tatttaatta ttcgcccccg gtctttacac
    10921 ggttttgttc ttctgaagca ccacctgttt ttttcttttg accgtttcca ttgatttttc
    10981 ctttgctgaa ccggtatgta aagccaattg ctgcctgcct tgaatccctt tgttgctgga
    11041 aagctgcatc tacatttcca tatttaatac tgccatttat cttttgggaa taaagcacat
    11101 cacgaatatt aaaacgcaat gacccctttc ctttgagaat ctgcttactg atccccatat
    11161 tcatcattcc aaaaccattg atcctgaaaa caccatccac accacctgtt ctgtagaatc
    11221 cgctcagctc ggcactccat gtctttgaga acttaaactg gtttgaggca ttgaactggc
    11281 ctgttgtggc tttgatatcc acctggtcac catttattat tccttcaaaa cgattattat
    11341 aaacatttgc atagatattg ccactccacc acttggtgat ctgacctcct gcacttactg
    11401 ctataccgta ctgacgttgt ttggctatat tctgattcct gatcacagtc tcgtttttat
    11461 caggatcctg ttccagtaca tcattaatta tatcggttgt tctggtataa ttcaatgtcg
    11521 tattcaaaaa tcctttaaat gtatgcgtca gctctatatt atgagagaat tgtggtttca
    11581 ggttaggatt tccctgttcg aatgtatacc tgtcgagaaa aatgataaac ggatttaaat
    11641 cttcatagtt aggacgattt atccgtcgac cataattaag aaccaaacta tgcttttcac
    11701 tggccttgta ttgaagaaaa gctgttggga acaactgggt atagttacgc ttgaacttct
    11761 ctcctgttgt tacctggttc ccgttcgcat tggtattctc cagtcttaca ccaaattgac
    11821 caaacaattt tttagtcaat ggcctgctat agttaacata agctgcattc acattttcat
    11881 catatataaa atgattggta cgcccaatat ccctaactct cacgccataa ttaatgctat
    11941 cataaattgc attattatcg gtattcacaa aacttgtttt aacacctgcc tcgaattttg
    12001 cacctttatt cagaggatgt gtgtaatcca ttttagcagt atatatattg atatcctgcg
    12061 gcaggctacc caaaagagta tcagctttaa tggtaggcaa cccatgtgca tcgtagtaag
    12121 cattaatcag gtcctggctg tttttactac gataagtcag gtaatcaaca tcaactgtta
    12181 actctctgcc tgttgaatca aatacatgac gtaaattcag gttagtactg aatcctttcc
    12241 atttcctgtc attacttgta cttgccaggg tttgactcaa taaagacatg gcaggatcat
    12301 agatcattac atcactgtgg ttcccaaatt taccaggatt ataaaaacct gaaaatacta
    12361 ctcccaaagt ggttttattt gaagcgaaat aatccaatcc caattttgca ttgtgggatt
    12421 tcccattgtc ccttattctt gacaattgtt cgaaatttga cttgacctct tttgtagatg
    12481 actcaataaa tttcctttgt atattaagat cctggaaatt tttcctgcta ttatagccaa
    12541 gtgtagaaaa gaagttcact ttatttttcc tgtaattaaa actaaatgac tcattcagct
    12601 ttgcataacg accctggctg taagtagaag ataaattacc attatagcca aattgtttgg
    12661 tcttctttgt ttttatatta attacaccgc tattaccggc agcgtcatac ttagctggtg
    12721 gattggtcat aatttcaatc tgatccaact gattactgct gaggctacgg agataattag
    12781 ccagatcggt acccgacaag tatgttgggc ggccatcaat ataaacctgc accccttgtt
    12841 ttcctttcag gctgatatta ccatctttat ccactgagat accaggagat ttttccagca
    12901 cttccatggc tgaggttccc acattggtta ctgatgcctc cacattaact atagtacggt
    12961 cgatcttttg ttcgatcaat ggctttttcg cggtaacggt taccccaccc agagattttg
    13021 cctggggtgt cagttcaatc gtttttaaag tcacaagggt agatacagga tttatctcaa
    13081 aaaccgcgga ataccctttg ctatgcccta ctgcgctaac cgaaaccata taagttcctt
    13141 caggaactac atcgaattca aacttcccgg ttttatctgc agcagtgatt tttgctacag
    13201 aagaatcttt cacccgcaat aaagtgatgg tggcggactc aattgttttg gcactgccgt
    13261 caattacggt gccttttact tttcctcctg taaactgggc ctggcttgta aaggataagg
    13321 cagttaagcc aaccgtcaac agagttaata attttctcat tttttcaatt tttataagta
    13381 agtacttgtt tttagttatt acaggtcaaa gattggtact ttgtattaca tagaacattg
    13441 ttttaaatta aacggcctat tttagggata atcggccgtt ccggattaca atagtgtgac
    13501 gcagtgcctt aaattgggtt acagaaatgc ccccatatca tataagtggc gactttttgg
    13561 gatgagcgga cagaatctct aaaatttggc caggttgacc gaaaaaggga aaaatgaggg
    13621 tttttgagac caggtttaaa ctcgaaaaag aggcgggtaa ggacaccaaa ttcatttata
    13681 aaccaaaatc tgatctaatc agatcatgat aagtgcggcc tgaggtaagc cggctgccca
    13741 ctttatcatc cattacaaac agataacgac cgttgaagtg acgatgcatt tccctgatcc
    13801 tgtccttatt tataatatat gatttttgaa tgcgataaaa ctgttggggc aatttttcag
    13861 atagtgcagc aagagaattg tcggtcaggt attttgttcc atcaacagta aaaatgtaaa
    13921 catatttatc ctgtgcttcc atataagcga tactctcata gcgcagaaga atgatcctgt
    13981 caccggtttt gataggcaat gcagttgcct tttgaggagc ctggaactgg cggataattt
    14041 cctgaaggcc tgatacatca atcgtgttgg aaatccttcc aaagcttttc agtttttgaa
    14101 tgctttgcgc taacctttct tccctgacag gttttaaaag ataatctatc gaaaatgttt
    14161 caaatgcttt gattgcatat tgttcatatg ccgttgtaaa aataatgttt ggcttatgtg
    14221 taagccgttc tatcacttca aagcctgtca ggtctggcat ttgtatatcc aaaaagatca
    14281 gatccggctt cagcgcttca attttttcaa tagcctctct cccgttaccg gcttcttcaa
    14341 tcactgaaac agtatcagca tatgccgaca acaggctttt cataatcctg cgggctgccg
    14401 gttcatcatc tattacaata gctttaaaga cctggctcat ttttcaacag tttattaatt
    14461 tggatcgaca cctgttttcg gggtacgtta ctgaaatgga tctcatattg ccctgggaaa
    14521 agaaggtcca gcttatcaaa aacactttta acgccatacc ctggtaccag ttcgtccgga
    14581 aagggcgggc cattgtcagc gatattgatg ataacgccgt tatcttttct cctgacttcc
    14641 aggaagatct ctgtcagttt acccgttgct ttcaatccgt gtttaacggc attctccgct
    14701 aatggctgga gaataaagcg ggggatctgg taatgtgcag tagcaggttc cacatagact
    14761 gaataagtca gctgatcttc aaaccttatt ttttcgatct gcatatagac ttcagtcata
    14821 gccatctcat cattgatggt ggaataatta ttctgactgt aattgatact ataacggaaa
    14881 agatctgcga gagctattgt catttttctt gccttcttac catccgtcat tgaaagatcg
    14941 gcgattgaat tcagggcatt atataaaaaa tgcggattta ctttagagtg cagtgcatcc
    15001 aattctgctt tagcctttaa ttcactcata cggctcagct caagttcttt cgcatcaaac
    15061 tttctttttc gctcaatatc aagataactg atcactgcat atatcaatcc ggttgcacct
    15121 gccaggtaaa tactgttaat aataatatac ttcaggtaag atgtttcgct cccgtaaagt
    15181 atataaccaa acggaatatt aatggcaaca gtagttacaa ttaaagaaaa aacgataagg
    15241 gcattatgct gaataaacgt cagcttctta aaccttttat tttctgctat aaaccttacg
    15301 gtgttgtaca ccacaagggt tgtagctgta gtagaaataa attggccaat aactgaccac
    15361 caccaacccc atttagccag catataaaaa aaccctccgc tcatcggcag cattgcggtt
    15421 gaaatacaaa aaatggccag ccatgccaca atattctttc tcaacaactc attcctgacc
    15481 gattgtttaa aaagcctggt aaagtttgtt aaaatgtaaa gacctgccag cgcaaataac
    15541 aggcaggaaa aaattagtat aatatattca agtgttgtag gttccttcat acaaaatgcc
    15601 aaaataagaa
//
LOCUS       DAORTI010000083        15396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3607_length_15396_cov_6.421224, whole genome shotgun sequence.
ACCESSION   DAORTI010000083 DAORTI010000000
VERSION     DAORTI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_15396_cov_6.421224"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="lepB"
                     /locus_tag="PLO70_10980"
     CDS             <1..980
                     /gene="lepB"
                     /locus_tag="PLO70_10980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQZ75034.1"
                     /translation="FGSSRKSYSEAIKLPYMRWFATPVKREDAVVFNFPAGDTVINLP
                     NFQSKDPYYDVCRRMGKGNIDEGRKIVLNDPGNYPLAVHPADKSDNYIKRCVAVAGDM
                     LEIKEGQVYINGQKTVPPPDAQYTYSFTSKVNLNEDFMKEEFNTNEVGIDANGNFTIN
                     LPYNMVDKFKSQPFLSSPLVPDNELYISRDVNDFDPRTTSGDVYPQDTVYFKWTKDNF
                     GPVWIPAKGASVQLNDSTYSLYERAIRVYEKNEFYKKDGKFYLNGKEVSNYSFKMNYY
                     WMMGDNRHGSQDSRFWGFVPEDRVVGKAWLIWFSWEGGPRWKRLFRVVK"
     gene            1042..1527
                     /locus_tag="PLO70_10985"
     CDS             1042..1527
                     /locus_tag="PLO70_10985"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQZ75035.1"
                     /translation="MKQGKFEFEYEVYDDISQLSNADAVLLNKARAVTAQAYAPYSKF
                     NVGAVAILDNGEFVAGTNQENASYPVGICAERVLLGNAATLYPGIAINTIAISYNPAK
                     EKSNHPISPCGMCRQSLLEYETRFKKPIRVIMGGMEGKIHIVKTARLLLPFAFTSEEL
                     I"
     gene            complement(1540..2037)
                     /locus_tag="PLO70_10990"
     CDS             complement(1540..2037)
                     /locus_tag="PLO70_10990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQZ75036.1"
                     /translation="MSDTFHTYYHSPVGLLKISGTEKYISEVSFHDTSQKAEGKKKHF
                     PLILINCVEQLIQYFNGQRRIFELPLNQPGTVFQQEVWNELVQIPFGKTISYLELARR
                     IGDFKATRAVASANGKNNIAIIVPCHRIIGSNKELIGYAGGLWRKKWLLEHEAKVAHG
                     VQTLF"
     gene            complement(2039..2374)
                     /locus_tag="PLO70_10995"
     CDS             complement(2039..2374)
                     /locus_tag="PLO70_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HQZ75037.1"
                     /translation="MSISWSDFEKIDIRIGTVIAVNDFPKARKPAYQLTIDFGSTIGI
                     KRSSAQITVHYPKELLLNRQVIAVVNFPPRQIANFISECLVLGIYDENNDVILLQPER
                     PVQNGQKIG"
     gene            2479..>3911
                     /locus_tag="PLO70_11000"
     CDS             2479..>3911
                     /locus_tag="PLO70_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ75038.1"
                     /translation="MTPVKKNMNLSRRNFIRNTAITGTGFFIVPRHVLGRGFIAPSDK
                     LNIAGIGAGGKGESDLLEFSKSPYVNIVAMCDVDDREAAKSRKRFEKANYYHDFREML
                     AKEKNNIDACSISTPDNTHAVATLAAMQLGKHVYTQKPLTHDIYEARILTEAAKKYKV
                     VTQMGNQGGSGDGVRKAKEMYEAGMIGKVTEAHAWTNRPVWPQGIPTPAGKYDIPKEL
                     DWDLWLGPAKGIDYNPAYLPFNWRGWWAFGTGALGDMACHIMDPIYRILPILYPDSAE
                     CSVATIWKEMWNDTQNPDACPPASIIHLNYPRTDGKGTIRVSWHDGGLLPERPEELLP
                     EEAFGNWDGGVLLIGTKGKMLLDCYGANPRLLPSRLMKEKTMPKETIKRVPEGHYLQW
                     VNACRAGYGKETTSSPFEYAGPFTESILMGNLAIRSWMMKNPKLKGWEDKYLGRKKLL
                     WDARNMKVTNFDEANQFVKREYREGYSL"
     assembly_gap    3912..4011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4301..5167)
                     /locus_tag="PLO70_11005"
     CDS             complement(4301..5167)
                     /locus_tag="PLO70_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQZ75039.1"
                     /translation="METNNEISALFTLIDDPDEEVFGLVSNKIVDYGKEIIPNLEHLW
                     ETTPNEEIQTRIELLIHRLHFTDLAEDFRQWSISGHHDLMLGAMLTSKFQYPEISTTP
                     VLQEIEKVRRNIWLELNNYLTPLEQINIVTSILYSYYGLKGGETNYKEPNEFLLHKTL
                     EVKRGNQISNGILYLVLCELLDIPVRAINIPKQFIVAYFKPGYSEENLSDPLHKVEFF
                     IDPTSGQIFTHQDVDNYFKRISVPPVGSYFKPRKNKQVVQQLLEEFSKCFDSDKEKHK
                     QAELMELVKLLD"
     gene            complement(5342..6088)
                     /locus_tag="PLO70_11010"
     CDS             complement(5342..6088)
                     /locus_tag="PLO70_11010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ75040.1"
                     /translation="MKRAYWEKIAPSYNDEIFDVLYNDTKALIRSVIDRYASKKKTAV
                     DIGCAIGKWLPVLSPAFKKVVAVDISAKNLAIAQQLYPQYKNVEYLRADMSGKKIKVP
                     PCDFGICINAILTPSQKDRDIFFRSLNACIKKGGRIVITIPSLESHLLTSIIQQQYKI
                     DKALFPAVKNGKEAIRKWNNIRQGNADIDEVTHKHYLKEELRLLLSKEGFVAEEFQKI
                     EYDWSTEFHNPPKWLKEPRPWDWMVVAKKK"
     gene            complement(6117..8696)
                     /gene="topA"
                     /locus_tag="PLO70_11015"
     CDS             complement(6117..8696)
                     /gene="topA"
                     /locus_tag="PLO70_11015"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQZ75041.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGSDFQVKSCFGHIRDLEKAGMGI
                     DLEKNFEPRYIVPDDKQKVVNELKSLAKKSGEVWLATDEDREGEAISWHLCEVLGLDP
                     KTTKRIVFHEITKPAIQAAVQNPRKLDMNLVMAQQARRILDRIVGFELSPVLWRKMSM
                     RNNLSAGRVQSVAVRLIAEREREINSFIPISTFKIEGIFTAKDVSDKPVTFAAEGKKQ
                     SKAEDAEAFLKSCAGAAYKVTDIQVKPGKRSPAPPFTTSTLQQEASRKLGYGVARTMQ
                     IAQRLYENGYITYMRTDSVNLSNTALSDITNTVKSMYGEEYHQFRKYKNKNESAQEAH
                     EAIRPTYMSNSTVPEPEWVRLYELIWKRTMACQMADAELEKTTARIEISTNKEELTAS
                     GEVLKFDGFLKVYREDKDDDELEEEANEGMLPPLTVGQQLPLKEMKATERFSRPLPRY
                     TEASLVKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGVRRDFRVFKLQKDIVSKI
                     MEQENTGAEKSKLFPSDLGLVVTDFLKQYFDNIMDYSFTARIEEEFDEVAEGKLKWSS
                     VIDEFYTPFKKDVDETIETAERIKGERVLGVDPVSGKPVVARMGRYGAMIQIGVADDE
                     EKPRFAKVPTGQSIETITFEEAMNLFGAQGTMGQYDEKDISVNVGRFGPYVKWGEEFI
                     SLPKGTDLGTVDLETAIKHIKQKQIADAPVGIYENKPITKGKGRFGPYIKWDSMFINV
                     PRRYNFDTLTQAEMNELIDAKVKKEANRFIQRWPDDKISVENARWGPILKFGKKIIRI
                     PKKADDTKYTAEEAAAFTLDDVKKFIEAEVPGAFTKKTRKTATKKAPSKKAAVKKKAA
                     KKK"
     gene            complement(8845..9453)
                     /locus_tag="PLO70_11020"
     CDS             complement(8845..9453)
                     /locus_tag="PLO70_11020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQZ75042.1"
                     /translation="MQSQSYTYLALGDSYTIGEMVPLTDSFPYQIVQLLRKEGFDFHA
                     PEIIAKTGWTTDELQVAVNNYQFLKTYDFVSLLIGVNNQYRGRNTDKFAAEFEALLEQ
                     AIQFARNESSHVVVLSIPDWGVTPFAEGRDREKIAKEIDIYNAVCREICLRFNIYYIN
                     ITPGTRETPADNSLLAVDGLHPSGKEYKRWAELVAAFFKSKL"
     gene            complement(9513..10364)
                     /locus_tag="PLO70_11025"
     CDS             complement(9513..10364)
                     /locus_tag="PLO70_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQZ75043.1"
                     /translation="MATMKLVQRIALRYYKTKFRLWAAVSKKKAAEKAFELFCTPQRR
                     NLKQPPRIFEQAEKLQFKLKGIIVNGWRWNHPAPRKALILHGFESSATNFDRYVKPLV
                     KKGYEVLAFDAPAHGRSGSKQINAPLYKKMILEVNKQYGPVKSFMAHSFGGLAVCLAL
                     EEISHTNDYRLVLVAPATETTTAIDTFFNFLKLDPSLRSEFEKVIVKKGGVSSSWYSI
                     KRAMKHIRAKVLWVHDEDDDTTPLSDVLKVKAENYPNVEFLITKGLGHRRIYRDNKVT
                     KTIIDFL"
     gene            10437..11198
                     /locus_tag="PLO70_11030"
     CDS             10437..11198
                     /locus_tag="PLO70_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent NADP oxidoreductase"
                     /protein_id="HQZ75044.1"
                     /translation="MDIVIIGSGNVAAVLGRKFKAAGHTILQIYSRNAAAASELAYEW
                     DTESTNYKITINKNADIYIIAVSDDAIDDVTADLKLPGKIVVHTAASVPKEVLKNVTT
                     HYGVFYPLQSLRKEMKSLPDTPIFYDGSDELTKRKLESLAKSILREKVTEAGDEARLK
                     LHIAAVVVSNFTNHLYALAEAFCRKEGLDFKQLLPLIEETALRIKDVSPHEAQTGPAA
                     RHDKETIQKHLELLKDHPQLKNIYLLLSESIQQEK"
     gene            11272..11607
                     /locus_tag="PLO70_11035"
     CDS             11272..11607
                     /locus_tag="PLO70_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQZ75045.1"
                     /translation="MYTIKFKFEQKGLSPVILNDVEPGQSLLEVALKNDIELHHNCGG
                     VCACSTCHLYVEKGAGFLEELSDREEDFIDRAVSPRLNSRLGCQCLLNEGEGEVEVTL
                     PDQTQFLGE"
     gene            11680..11919
                     /gene="iscX"
                     /locus_tag="PLO70_11040"
     CDS             11680..11919
                     /gene="iscX"
                     /locus_tag="PLO70_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HQZ75046.1"
                     /translation="MSHFEPPINWSDHEDIALKLYERFGDDFNEGKIYRIRFTDLLEW
                     VLQIPGFEGKREESNEGHLEMIQSAWVYEWRDNQK"
     gene            11985..12506
                     /locus_tag="PLO70_11045"
     CDS             11985..12506
                     /locus_tag="PLO70_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQZ75047.1"
                     /translation="MNVLELFKKITTFVFDVDGVLTDGTVMVLDNGLQARRMHIKDGF
                     ALQMALKNGYRVKVISGGNSPQVKDRLEKLGVTNIDMSVLNKQALLSAYMAVNKLKKE
                     EVLYMGDDLPDIGAMMEAGLPCCPADAVNEVKEAAKYISPANGGFACVRDVIEKVLKL
                     NDHWHYREDVTSR"
     gene            12572..13504
                     /locus_tag="PLO70_11050"
     CDS             12572..13504
                     /locus_tag="PLO70_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HQZ75048.1"
                     /translation="MRLIAGFFKLIRLPNLIFIALTQVLFQFCIYYTLYKGNIPSDDL
                     SRFIALLFASLFIAAAGYVINDYFDINIDEVNKPQKLVVDRIISRRWAIAWHFMLSAS
                     GIILTALAVHFMQKWYLILANIFCVALLWVYSTNFKKSLLFGNIVISILVAWTILIIF
                     FSKLSVADAVGMGEGAHYKFFRLAFLYAGFAFIATLIREAIKDIEDMPGDLKYGCRTM
                     PIVWGVNAAKIYIAVWLIVLIAILIVVQVYVLQFRWWLPVLYSIGLIIAPLVFIFSKL
                     FRAVSTADFHQLSNWIKLVMLTGILSMLFFYFYL"
     gene            13501..14064
                     /locus_tag="PLO70_11055"
     CDS             13501..14064
                     /locus_tag="PLO70_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HQZ75049.1"
                     /translation="MNKIILASQSPRRKQLLEWAEVPFEVVVKETDERFPPGLTPEEV
                     AIYIAKNKAMAVQATRNSNEVIMAADTIVVLGDNIIGKPVHREDAVSILLALSGEKHK
                     VITGVVIRKGEEEIAFADTTDVEFHDLSVKEIEFYVDKYKPYDKAGAYAIQEWIGVVG
                     IKTISGDFYNVMGLPVSRVVKELKKIE"
     gene            14149..>15396
                     /locus_tag="PLO70_11060"
     CDS             14149..>15396
                     /locus_tag="PLO70_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HQZ75050.1"
                     /translation="MTERLLQFIWQFQYFTKSELSTTAGELIQIIYPGQYNTNQGPDF
                     ADAKIKIGKTTWAGTVELHINTSDWTKHNHQQDKNYNNVILHVVWKDDKAGNNIPVLE
                     LKGRVSKILLYRYEELMHADSFIPCEKSIHSVREIVCKSWKDRLLAERLIRKAAIVDN
                     YLQQNNYHWEETFWWLLARNFGMKVNTEAFEAIARSVPVNILAKHKNQVHQLEALLLG
                     QAGLLQDKFTDDYPLLLQKEYRFLQSKYRLKPVSLPLHFLRMRPGNFPTIRLAQLTML
                     VHQSVHLFSKIKEVHSVKEIKQWFDVTANDYWHYHYRFAERSAFKKKKLGADMIDNII
                     INTIVPVLFAYGSYHKEQQYKDKALQWLGGIAAEVNRITKGFLQLGIENKNAFDSQAL
                     IELKNEYCSKKRCLDCGIGNALLK"
BASE COUNT         4327 a         3000 c         3310 g         4659 t
ORIGIN      
        1 catttggttc cagccggaag tcctattcag aggcaattaa gcttccttat atgagatggt
       61 ttgcgactcc ggttaaaaga gaagatgcag tagtatttaa ttttccggca ggggataccg
      121 ttataaatct gcccaatttc cagtcgaagg atccctatta tgatgtatgc aggagaatgg
      181 gtaaaggaaa tatagatgaa ggaagaaaaa ttgtattgaa cgaccccggc aattatccgc
      241 tcgctgttca ccctgctgat aaaagtgata actatattaa acgttgcgta gctgttgccg
      301 gcgatatgct cgaaattaaa gaagggcagg tctatatcaa cgggcaaaaa acagttccgc
      361 cgcctgatgc acagtacacc tacagcttta catccaaagt gaatctgaat gaagatttta
      421 tgaaagaaga atttaatacc aacgaggtcg gtattgatgc aaacggaaac ttcacgatca
      481 accttccgta taatatggtt gataaattca aaagccagcc ttttctttcc agccctcttg
      541 tccccgataa tgaattgtat atttcaaggg atgttaatga ttttgatccg agaacgactt
      601 ccggtgatgt gtatccgcag gatacagttt attttaaatg gacaaaagat aattttggcc
      661 ctgtatggat acctgcaaaa ggagcttctg tacagttgaa tgattcaact tattcgttgt
      721 atgagagagc tatcagggta tatgaaaaaa atgaatttta taaaaaggat ggcaagttct
      781 acctgaatgg caaagaagtg agtaattata gttttaaaat gaattattac tggatgatgg
      841 gagataatcg tcatggttcg caggattcaa ggttctgggg ttttgtacca gaggacagag
      901 tagtgggtaa agcatggctg atctggttca gttgggaagg tgggccaaga tggaaacggt
      961 tgttccgtgt tgtaaaataa actaacttta attgcccttg actatgtcag gggcattgtt
     1021 gtctttaaac gaatcggaaa aatgaagcaa ggcaaatttg agtttgaata cgaagtatat
     1081 gatgatatca gccagttgag taacgcagat gctgtactgc tgaataaagc aagggctgta
     1141 acggcacagg cttatgcacc ttattccaaa tttaatgtag gggctgtagc catcctggat
     1201 aatggtgaat ttgttgcagg taccaaccag gagaatgcct cttacccggt gggaatttgt
     1261 gcggaaagag tattattggg aaacgcagcc acactatatc ctggtattgc gataaacaca
     1321 atcgctatca gttataaccc ggccaaagaa aaaagtaacc atccaatttc tccctgtggt
     1381 atgtgccgtc aatcgctttt ggaatatgaa acaagattta aaaaacctat ccgtgtcatt
     1441 atgggaggaa tggaagggaa gatacatatc gttaaaacag cacgcctttt attgcctttt
     1501 gcttttacca gtgaggagct gatttaattg gaaatctgtt taaaacaatg tttgtactcc
     1561 atgcgccact tttgcctcat gttccagtaa ccatttcttt cgccacaacc cgccggcata
     1621 gccaatcagt tctttattgg aaccgattat ccgatggcag ggtacaatga tggcaatatt
     1681 atttttcccg tttgcactgg ccactgctct agtagccttg aaatcgccga ttcttctggc
     1741 taattcaaga tagctgatcg ttttgccaaa aggtatttgc accagttcat tccatacttc
     1801 ctgctgaaat acagtacccg gctgattcaa tgggagctca aaaatcctgc gctggccatt
     1861 gaaatactgt atcaattgtt caacacaatt aattagtatc agtggaaagt gttttttctt
     1921 tccttcggct ttctgtgaag tgtcatgaaa gctcacttca cttatatatt tttctgtacc
     1981 gcttattttg agcaggccaa caggagaatg gtaatatgta tggaaagtgt cggacatctt
     2041 aaccgatctt ttgcccgttt tgtacgggtc gttcaggctg caataaaatt acatcgttat
     2101 tctcatcata aatgcctaaa acaagacatt cactgataaa gtttgcaatt tgccggggag
     2161 ggaagttaac aacggctatg acctggcggt tcagcaataa ttctttgggg taatgaactg
     2221 ttatttgggc ggaggacctt ttaatcccaa tagtactgcc aaagtcaatg gttaactggt
     2281 aagccggctt tctagctttt ggaaaatcat tgaccgcaat gacagttccg atccttatgt
     2341 caattttctc aaaatcactc cagctgatgg acatttcttt tttctgaaag ctaaagattt
     2401 gttgtgaaag ttgatgaaat acagaataat ttttgacggt tatacaagtt caatttaact
     2461 taatgaaaat tttccggtat gacgcctgtt aaaaagaata tgaatctttc ccgcagaaat
     2521 tttatccgta atacagctat tactggtact ggttttttca tagttccccg gcatgtactg
     2581 ggccgtggat ttattgctcc cagtgataaa ttgaatattg ccggaatagg ggcaggcgga
     2641 aaaggagaaa gtgatttgct ggagttttca aaaagccctt atgtaaatat tgtggccatg
     2701 tgtgatgtag acgaccggga agctgcaaaa tcaaggaaac gatttgaaaa agcaaattac
     2761 tatcatgact tcagggagat gctggcgaaa gaaaaaaata atattgatgc ctgttcaatt
     2821 tctacacctg ataataccca tgctgtcgcc acactggctg ccatgcaact gggtaaacat
     2881 gtgtatacgc aaaaaccgct tacgcatgat atttatgaag caaggattct gactgaagcg
     2941 gctaaaaaat ataaagtcgt aacccagatg ggcaaccagg gtggttccgg tgatggtgta
     3001 cgtaaggcga aagaaatgta tgaggcaggg atgatcggta aagtaaccga agctcatgca
     3061 tggacgaatc gtccggtatg gccacaaggg atacctactc ctgcaggaaa gtatgatatt
     3121 ccgaaagagc ttgattggga tttatggtta ggtcccgcta aagggattga ttataacccc
     3181 gcttacctgc cgttcaactg gcggggatgg tgggcctttg ggaccggtgc attaggagac
     3241 atggcctgtc atataatgga tccaatttat cgcattcttc cgattcttta tcctgattct
     3301 gctgaatgca gtgtagctac catctggaaa gaaatgtgga atgatacgca aaacccggat
     3361 gcttgtccgc cggcttccat tattcatttg aattatccaa gaacagatgg gaagggtaca
     3421 atcagggtat cgtggcatga tggtggacta ttgccagaac gtccggaaga attattaccc
     3481 gaagaagctt ttggaaactg ggatggtggg gttttattaa ttggtaccaa aggtaaaatg
     3541 ttattggatt gctatggcgc aaatccaaga ttactgcctt ccaggctgat gaaagaaaag
     3601 acaatgccga aagaaaccat caaaagggta cctgaaggcc attacctgca gtgggtgaat
     3661 gcctgccgtg ccgggtacgg taaagaaaca actagttctc cgtttgaata tgccggaccg
     3721 tttacggaaa gtattttgat gggtaacctg gctatccgca gctggatgat gaagaatcct
     3781 aaactgaaag gatgggaaga caaatatctt ggccgtaaga aacttttgtg ggatgccagg
     3841 aacatgaagg ttaccaactt tgatgaagcc aaccagtttg taaagcggga atatagagaa
     3901 gggtatagcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntactttata
     4021 attgtatgtt gtcaccctga gcttgacgaa gggttgatga agccaaccag tttgtaaagc
     4081 gggaatatag agaagggtat agccttactt tataattgta tgttgtcacc ctgagcttga
     4141 cgaagggttg atgaagccaa tcagtttgtg aaaagcgaat atcgggatgg gtggaaattt
     4201 gagctataat ctgaggtaat agaagtgcag tgtaacaaaa cccaaaacac tgtattttta
     4261 aaatctcctg agcctaacct taaaaagagt attttgaata ttaatcaagc agcttcacca
     4321 gttccatcaa ttcagcctgc ttgtgctttt ctttatcgct gtcaaagcat ttactgaatt
     4381 cttccagaag ttgttggact acctgtttat tttttcgggg tttgaaataa gaacctaccg
     4441 gaggaacgga gatgcgtttg aaataattgt ccacatcctg gtgggtgaag atctgccctg
     4501 aagtggggtc aataaaaaac tccactttgt gcaggggatc agagaggttc tcttcggaat
     4561 aacctggctt gaaataagca acaataaatt gtttgggtat attaatggct ctcaccggta
     4621 tatccagtag ttcacacaac accaggtaga ggatgccatt acttatctgg ttgcctcttt
     4681 ttacttccag cgttttgtgc agcaaaaatt cattcggttc cttataattg gtttcgccac
     4741 ctttcaggcc ataataactg tataaaatac tggtgacgat attgatctgc tccagcgggg
     4801 tgagataatt attcagttca agccagatat tacgtcttac tttctctatt tcctgcaaga
     4861 ccggcgtagt ggaaatttca gggtattgaa actttgaagt cagcatagca ccgagcatca
     4921 ggtcatgatg accggaaata ctccattgcc ggaaatcttc tgcaaggtct gtaaaatgta
     4981 gacggtgaat cagtaactcg atcctggttt gtatttcttc gttgggggtt gtttcccaaa
     5041 gatgctctaa gttgggaata atctctttac cataatcaac gatcttattg gatacaaggc
     5101 caaagacctc ttcatcagga tcatcaatca acgtaaacag tgcagatatt tcattattgg
     5161 tttccatggt agtgattctg gcacaatttc tttattactg atgaaaggga atagttaaac
     5221 ttttccacag atggggagaa gctgaataaa tcaaagtttg aagttggatg ctgtttgtta
     5281 actgagtgaa aagctaagct tcagatttaa gaaacaaacc gggacttgca gtttcaaaaa
     5341 cttatttttt tttagctact accatccagt cccatggcct tggctctttc agccattttg
     5401 gaggattgtg aaattcagtt gaccagtcat attcaatttt ttgaaattct tcagccacaa
     5461 aaccttcttt ggataataat aaccggagtt cttctttcag gtaatgttta tgtgtgacct
     5521 catcaatatc agcattgccc tgccggatat tattccattt gcggatggct tctttcccgt
     5581 tctttacagc agggaaaagg gccttgtcaa ttttatattg ctgctgaatg atgctggtca
     5641 ataaatgtga ttccagcgaa ggaatggtga taacaattct tccgcccttt ttgatacagg
     5701 catttaacga ccggaaaaat atatccctgt ctttttgtga tggagtaaga atagcattga
     5761 tgcagatacc aaaatcacat ggaggtactt ttattttctt cccgctcata tcagcccgca
     5821 ggtattctac atttttatat tgcggataga gttgttgtgc tattgcaaga tttttcgctg
     5881 aaatgtcaac cgccacaact tttttaaaag caggggaaag taccggcagc catttaccaa
     5941 tggcgcagcc gatgtcaacc gcagtctttt tcttcgatgc atacctgtca atcactgatc
     6001 tgatcaatgc tttcgtatca ttgtacagta catcgaatat ttcatcgttg taggaaggcg
     6061 ctattttttc ccagtaggct cttttcatct gtaattgaat acaatgctta caatgattat
     6121 ttctttttag cagccttttt ctttactgca gccttttttg acggagcttt cttagttgct
     6181 gtcttcctgg tcttcttcgt aaatgcaccc ggcacttccg cctcaatgaa tttcttcaca
     6241 tcgtctaagg taaatgcagc agcttcctcc gctgtatatt tcgtatcatc tgctttttta
     6301 ggaatacgaa tgatcttttt gccaaactta aggataggcc cccatctggc attctctaca
     6361 gagatcttat catccggcca gcgctggatg aagcgattgg cttctttttt tactttggca
     6421 tcaatcagtt cgttcatctc tgcctgggtc aacgtatcaa aattgtaacg tcttggtaca
     6481 ttgataaaca tgctatccca tttgatgtaa ggaccaaacc ttccttttcc ctttgtgatg
     6541 ggtttgttct catagatacc cactggtgca tctgcaattt gcttttgctt aatatgtttg
     6601 atggcagttt ctaaatccac ggtaccgaga tctgttccct tgggcagcga aataaattcc
     6661 tcaccccatt ttacataagg accaaaacgt cctacattaa ctgaaatgtc tttttcgtca
     6721 tactggccca tggtaccctg tgcaccaaac aaattcatcg cttcttcaaa tgtgatcgtc
     6781 tcaatacttt gtcccgttgg gaccttggcg aagcggggtt tttcttcatc atcggcgaca
     6841 cctatttgaa tcattgcgcc atagcgtccc attcttgcta caaccggttt accgcttacg
     6901 ggatcaactc ctaatactct ttcccctttt atacgttctg ctgtttctat agtttcgtcc
     6961 acatcctttt taaaaggagt gtaaaactcg tcaatcacac tgctccattt taattttcct
     7021 tccgctactt catcaaactc ttcttcaatc ctggctgtaa aactgtaatc catgatgttg
     7081 tcaaagtatt gtttcaaaaa gtcagtgaca actaatccga gatcagaagg aaacaatttt
     7141 gatttctctg ctcctgtgtt ttcctgttcc attatcttac tcactatgtc cttctgtaat
     7201 ttgaacaccc ggaaatccct ccggacaccc tctttatctc ttttctctac ataacctctc
     7261 tttaatatgg tagaaattgt gggagcataa gtggaaggcc ggcctatacc gagttcttcc
     7321 agttttttta ccaatgaagc ttcagtgtat cttggcagtg gccggctgaa acgttccgtc
     7381 gctttcattt ctttcagtgg caactgctga ccaactgtca acggaggcaa catcccttcg
     7441 ttagcttctt cttccagttc atcatcatcc ttgtcttccc ggtacacttt taagaagcca
     7501 tcgaatttca acacttcacc actggcggta agttcttcct tattagtgga gatttcaatc
     7561 ctggcagttg ttttttccaa ttcagcatct gccatctggc aggccatcgt tcttttccag
     7621 atcagttcat acaaacgtac ccattccggt tccggtacag ttgaattact catataggtt
     7681 ggccgtattg cttcgtgggc ttcctgggca ctttcattct tgtttttgta tttccggaac
     7741 tggtgatatt cttcaccata catgcttttt accgtattgg taatatcact caatgcagta
     7801 ttacttaagt tcacactatc agtacgcata taagtaatat atccattctc atagaggcgc
     7861 tgtgcgatct gcatcgttct tgctacaccg taacccagtt tccttgatgc ttcctgttgt
     7921 aacgtggaag tagtgaaagg cggggcaggg gatcgtttac ccggttttac ctgtatatca
     7981 gttactttat aagctgcgcc agcacagctt tttaaaaaag cttcagcatc ttcagcctta
     8041 ctttgttttt tcccttcagc tgcaaatgtt acgggtttat ctgaaacatc tttggcggta
     8101 aatatgcctt ctattttaaa ggtgctaatg ggtataaagg aattgatctc tctttctctt
     8161 tcagcaatta accggactgc tacactttgt acacggcctg cactcaggtt gtttcgcata
     8221 ctcatttttc gccatagaac cgggctcagt tcaaatccca cgatcctgtc taggatcctt
     8281 cttgcctgtt gcgccataac aaggttcata tccagtttcc tgggattctg gacggcagcc
     8341 tggatagccg gttttgttat ttcatggaat acaatgcgtt tggttgtttt gggatcaaga
     8401 cccaatactt cacataaatg ccagctgatg gcttctcctt cacggtcctc atccgttgcc
     8461 agccatactt caccagattt ttttgccagt gatttcagct cattcactac tttttgctta
     8521 tcgtccggta ctatataacg gggttcaaaa ttcttctcca gatcaatacc cattccggct
     8581 ttctccaggt cacggatatg gccaaaacaa ctctttacct ggaaatcact tcccagtatt
     8641 ttttcaatcg ttttagcttt cgcagggctc tctactatca ataaattttt cgccataaag
     8701 gatacaatat tatactacaa aaccgctttt cagcggccgc aagttaggac aaggataata
     8761 aatttttccg gaaaccttat tttatgatac caaaaacagt aaaatggcac gcataaatac
     8821 agcgaatatc tcgttgaatt tcaattataa tttagattta aagaaagccg caaccagttc
     8881 tgcccagcgt ttatattctt tgccggaagg atgtaacccg tcaacagcca atagtgaatt
     8941 atcagcaggt gtttctctag tcccgggagt gatattaatg taatatatat taaatcgcag
     9001 gcagatttcc ctgcaaacag cgttgtagat gtcaatttct ttggcaatct tttccctgtc
     9061 acgaccctct gcaaaaggag taacccccca atctggtatt gaaagaacga caacatggct
     9121 actctcgtta cgggcaaact gaatggcctg ttctaataaa gcttcaaatt cagcagcaaa
     9181 tttatcagta tttcgtcccc tgtattggtt atttacacca atgaggagac ttacaaaatc
     9241 atacgttttg agaaactgat aattattgac tgcaacctgc aactcatcag ttgtccagcc
     9301 ggttttggca atgatttcag gagcatggaa atcaaaccct tccttacgaa gcagctgaac
     9361 aatttggtag gggaaactat ctgttaacgg aaccatttcc cctattgtat aagagtcacc
     9421 tagtgctaaa taagtatatg actggctttg cattagaatt tgctttttat ctcagggcaa
     9481 tgaaagcgta agtaaaatta agaggaaggg cttcataaaa aatcaataat ggtttttgtc
     9541 actttattat cccggtaaat ccggcgatgc cccagtcctt tagtaattaa gaattcaaca
     9601 ttgggataat tttcagcttt tacttttaat acatcactca acggggttgt atcatcgtcc
     9661 tcatcatgta cccacagtac ttttgcccgg atatgtttca tagctctttt tatggaatac
     9721 caggatgagc ttactccacc ttttttcact attacttttt caaattcact ccgtaaggaa
     9781 gggtccagtt taagaaagtt aaaaaatgtg tcaatagctg ttgtggtttc agttgcaggg
     9841 gcaaccagca ccaaccggta atcattggta tgacttattt cctccaaagc aaggcaaact
     9901 gctaatccgc caaaagagtg tgccatgaat gatttaaccg gaccatactg tttattgacc
     9961 tcaaggatca tttttttata taaaggagca ttgatctgtt tgctgccgct tcgtccatgt
    10021 gccggggcat caaaggccag tacttcatag ccctttttga ccaggggctt cacataacgg
    10081 tcaaagttgg tggcggagga ttcaaatcca tggaggatca gtgcttttcg tggggccgga
    10141 tggttccagc gccagccatt aacgataatg cctttaagtt tgaattggag tttttcagct
    10201 tgttcaaaaa tcctgggtgg ttgtttcagg tttcgccgtt gcggggtgca aaaaagttca
    10261 aatgccttct ccgccgcttt ctttttggag acagctgccc ataacctgaa ttttgttttg
    10321 taatacctga gtgcaatccg ctgaaccagc ttcatagttg ccattaccgt taattttctt
    10381 tggtgtaatt tgcaaagcta ttcaatcatt cattaaactc ctttcaggat taaggtatgg
    10441 atatagtaat aataggttca ggaaatgtgg cagcagtgct gggcagaaaa tttaaagctg
    10501 caggacatac tattcttcag atttacagca gaaatgctgc cgcggcatct gagctggctt
    10561 atgaatggga tacagaatcg actaattaca aaattacgat caacaaaaat gcagatatat
    10621 atatcatagc tgtaagtgat gatgcgattg atgatgtaac tgccgatttg aaattacccg
    10681 gaaaaatagt tgttcataca gctgcctctg tcccgaaaga agttttaaaa aatgtaacaa
    10741 cacattacgg ggtattttat ccgctgcaaa gcctccggaa agaaatgaaa agcttaccgg
    10801 atacacccat tttttatgat ggcagtgacg agctgaccaa aagaaaactg gaatcactgg
    10861 ctaaatcaat attgagagaa aaggttacag aagccgggga tgaggccaga ttaaaattac
    10921 atatagctgc agttgtggtc agtaatttta caaatcatct ttatgcactt gcagaagcat
    10981 tctgtcgtaa agaagggctt gattttaagc aattgttgcc gctgattgaa gaaacagctt
    11041 taaggataaa agatgtttct cctcatgaag cacagacagg tcctgctgcc aggcatgata
    11101 aggagacgat ccagaaacac ctggaattgc ttaaagatca tccgcagtta aaaaatattt
    11161 acctgctatt aagcgaaagt attcagcagg aaaagtgatt gttttcccga acaagtaaac
    11221 cgtttcgttg tttttctgat acttttgccc ctcgaaacga aaactgaata gatgtacacg
    11281 attaaattta aatttgaaca gaaaggtctg tcacctgtga ttttgaatga tgttgaaccc
    11341 ggccaatcgc tgctggaagt ggccttgaag aatgatattg aacttcatca caactgtggc
    11401 ggtgtttgtg cctgcagtac ctgtcatttg tatgtggaaa agggtgccgg ctttctcgaa
    11461 gaacttagtg acagggaaga agattttatt gacagggcag taagcccaag gttaaattcc
    11521 cgcttgggtt gccagtgcct gctgaatgaa ggggaaggcg aggtagaagt gacattgcct
    11581 gaccaaacac aatttttagg agaataatta tcagaagagg tcagaagcaa gaagtctgga
    11641 ttctgacctc cggcatcata catcagactg ttgaattata tgagtcattt tgaaccaccg
    11701 ataaactgga gcgaccacga agacattgcc ctgaaactgt atgaacgttt tggtgatgat
    11761 ttcaatgagg gtaaaattta ccgtatccgt ttcacagacc tgctcgaatg ggtattgcaa
    11821 ataccgggtt ttgagggtaa aagagaagaa agtaatgaag gccacctcga aatgatacaa
    11881 agtgcatggg tgtatgagtg gagggataac cagaaatagt ttagtggttt ataagttgac
    11941 tggttaactt gtcaacttat aaacctgtca acccctttac aattatgaat gttctcgaat
    12001 tatttaaaaa aataaccact ttcgtttttg atgtggatgg ggtactgacc gatggtaccg
    12061 taatggtatt ggataatggt ctccaggcaa ggcgtatgca tattaaagat gggtttgcgt
    12121 tacagatggc attgaaaaat ggctatcgtg taaaggttat ttccggtggt aattcaccgc
    12181 aggtgaaaga ccgcctggaa aagcttgggg ttacaaatat tgatatgtct gttttgaata
    12241 agcaggcact gctttcagca tatatggctg tgaataaact gaaaaaagag gaagtactgt
    12301 atatggggga tgatcttcct gatattggtg cgatgatgga ggctggctta ccctgttgtc
    12361 cggctgatgc tgttaacgaa gtgaaggaag cagcaaaata catctctccg gcaaatgggg
    12421 gttttgcctg tgtaagggat gttatagaaa aagtgctgaa actaaacgat cactggcact
    12481 atcgtgagga tgtgacttcc cgctgataac tattatccgc ttcatatttc ttacttcaca
    12541 cttgtattgt atctttccgt aaattataat catgagattg atagcaggtt tttttaaact
    12601 aattcggctg cccaatctca tttttattgc acttacccag gtacttttcc agttctgcat
    12661 ttattatact ctctataagg ggaacattcc ttcagatgat ctgtcccggt ttatagcatt
    12721 attatttgcg tcactgttca tcgctgccgc cggatatgtg atcaatgatt attttgatat
    12781 caatattgat gaggtgaata agccacagaa gctggtagtg gatagaatta tcagccgtcg
    12841 ctgggccatt gcctggcatt tcatgcttag tgccagcggt ataattctta ctgcgttagc
    12901 tgtacatttt atgcagaaat ggtatttgat actggctaat attttctgcg tcgctcttct
    12961 ttgggtttat tccaccaact ttaagaaaag tcttcttttc ggcaacatcg ttatttccat
    13021 cctcgtggcg tggacaatcc ttatcatctt tttttcaaag ttatccgtag cagatgcggt
    13081 tgggatggga gaaggggcgc attacaaatt tttcagactt gcttttctct atgccggttt
    13141 tgcatttatt gccacattga tacgggaagc aataaaagat atagaagata tgccgggtga
    13201 tcttaagtat ggttgcagga ccatgcccat tgtttggggt gtcaatgccg ctaaaattta
    13261 catcgctgtg tggctgatcg tgttgatcgc tattcttatt gttgtacagg tatatgtatt
    13321 gcagttcagg tggtggttgc ctgtattgta cagtattggc ctgatcattg ctccgctggt
    13381 atttattttt tcaaaattat tcagggctgt ttctactgca gacttccatc agttaagtaa
    13441 ctggataaaa ctggttatgc tcaccggcat tctttctatg ctatttttct atttctattt
    13501 atgaataaaa taattcttgc ttcgcaatct ccccgtcgta aacaattgct tgaatgggca
    13561 gaagtgcctt ttgaagtggt ggtcaaagaa acggatgaac gatttccgcc gggtttgaca
    13621 ccggaggaag tggccattta tattgcaaaa aataaagcta tggcggttca ggcaacccgg
    13681 aacagcaacg aagtgattat ggctgcagat acgattgttg tattgggaga taacatcata
    13741 ggtaaaccgg tacaccggga ggatgccgtc agtatactgc tggccctgtc tggtgaaaag
    13801 cacaaagtaa taacaggtgt ggtaatcagg aaaggagagg aagaaattgc ttttgcagat
    13861 acaaccgatg ttgaatttca tgatctgtca gtaaaggaga ttgaatttta tgtggacaag
    13921 tacaagccct atgataaggc tggtgcctac gccatacagg aatggatcgg tgtggtaggg
    13981 atcaaaacta tcagcggcga tttttataat gtaatgggtt tgcctgtaag ccgggtagta
    14041 aaggagttga aaaagataga gtgagtaaat agggtgaaaa gggaatgagg tttgggatcc
    14101 gggattcagg gattttcttt aacttaaatt ccataaacct tatcaccaat gacagaaagg
    14161 ctgctgcagt tcatctggca atttcaatac tttactaagt cggaactgtc tactactgcc
    14221 ggggaactta tacaaattat ctacccgggt cagtacaaca ctaaccaggg acctgatttt
    14281 gccgatgcaa aaattaagat cggcaaaact acctgggctg gaaccgttga attgcatatc
    14341 aacacttcag actggacaaa acacaatcat cagcaggata aaaattacaa taatgttatt
    14401 ctgcatgtgg tgtggaagga tgacaaagca ggaaataata tacctgtatt ggaattaaaa
    14461 ggcagggttt ctaaaatact attgtatcgt tatgaagaac tgatgcatgc tgattcattc
    14521 atcccttgtg aaaagagtat tcattctgtg cgggaaatag tctgcaaaag ctggaaagac
    14581 aggctcctgg cagaacgctt aatacggaag gccgcaatag ttgataatta cttacagcaa
    14641 aacaattatc attgggagga aactttctgg tggctgctgg ctcgaaattt tggtatgaag
    14701 gttaataccg aagcatttga agcgatagcc cgttctgtgc ctgtcaacat actggcaaaa
    14761 cataaaaacc aggtgcacca gctggaagca ttgctgctgg gacaggctgg tttgctgcaa
    14821 gataagttta cagatgatta tccgctcctg ctgcagaaag agtatcgttt tctgcaaagc
    14881 aagtaccggt taaaaccagt ttcattacca cttcattttc tgcgaatgag acccggaaac
    14941 tttccgacaa tacggctggc gcaattaacg atgctggtac atcaatcagt acacttattt
    15001 tctaaaataa aggaagttca ttcggtaaaa gagatcaaac aatggttcga tgtcaccgct
    15061 aacgattact ggcattatca ttaccggttt gctgaacgat cagcatttaa aaagaaaaag
    15121 cttggtgcgg acatgatcga taatatcatt atcaatacca ttgtgcctgt cttatttgct
    15181 tatggcagtt accataagga gcaacagtat aaggacaaag cccttcagtg gctcggggga
    15241 attgctgcag aagtgaaccg tatcaccaaa gggtttctgc aactgggtat tgagaataag
    15301 aatgcttttg acagccaggc attgatcgag ctgaagaatg agtattgcag taagaagaga
    15361 tgtttggatt gcgggattgg gaatgcgttg ctgaaa
//
LOCUS       DAORTI010000084        15195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3697_length_15195_cov_6.855548, whole genome shotgun sequence.
ACCESSION   DAORTI010000084 DAORTI010000000
VERSION     DAORTI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15195
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_15195_cov_6.855548"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3329)
                     /locus_tag="PLO70_11065"
     CDS             complement(<1..3329)
                     /locus_tag="PLO70_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HQZ75051.1"
                     /translation="MKMQTVRTFFLTVSLFSLFAMNAGSQQPIKNYEKEWKKVEAFNK
                     KELPKSALVEVKKIYVLAKKEKQDAQVIKSLVYMTVLQSENREDNEIFSFAEVEKEIS
                     LSKEPVTAILKSILAEMHWNYYQQHRWQLYDRTKTEKFNKTDIATWDAVDFHKKIGEL
                     YLQSIKEEKLLQQTKLEPFDAIIIRGNIRHLRPTLYDLLAHRAISYFENDERDINKPA
                     YAFEIDQASAFDPAADFVKRKFTTKDSLSLQHKALLIYQKLINIHLADKKPDALIDAD
                     IKRIEFVNTNSTHPDKDKLYFNAINHIAHQYENMPAASQAWYLLAQYYNEKADSYKPN
                     GDTTYRFDRIKAKEICERVLQQTDSSEGKINAYNLLNAINSKSLQFTVEKVNVPGQSF
                     RSLVNYKNINTLYLRIIKADEKLKELLMNQYDEKYWPAIISATPFKHWQQQLPATNDL
                     QEHSVEIKIDGLATGEYILVASDKKELDGNKTIIGARFFYVSGISYVHNSNDYFVLNR
                     DNGQPIAKALVQVWEQKYDYKQSKYIKEKGKSYTTDANGFFILDKAKKESNNYGNYSY
                     LIDIRHEADRLFMNDLAYYYYYNNQPVETKPVTSVHLFTDRAIYRPGQTVYFKGIVLS
                     RNQKDKTGSVNADYSTTVVLRDANYKDADTLNLKTNEFGSFNGKFQLPQTGMNGQFSI
                     YTRKDGGNAGFKVEEYKRPKFYVEYETIKGTYKVNDTILVTGIAKAYAGNNIDGATVK
                     YRVVRNPRFIYPWLFWRWWRPPGEPMEIAHGEVKTDKDGKFAVKFTAIPDLKIEKKFE
                     PVFDYTVYADVTDINGETRSGEKSVSVSYKSLILLTSIPSTLPADSLKTLSIRTQNLN
                     GEYEPAVVKVTIHKLVEEKRLIRDRYWERPDQFVMTKDEYIRNFPYDEYDNESDYKSW
                     EKGEKIFEKTDSVKMNGDWSVVNGEYSPGFYIIEIATTDKNGEAVNDLKYIELFDEKS
                     KQLNRPDYLWTGVKKTTAEPGETAKVELGTTADNLFVVQQLDNQTGIQDPSVHKYSFL
                     KLHQEKKTFSFPVTEADRGGYGAGWMFIKHNRFYQLNQLINVPWTNKDLGIEYTTYRD
                     KTLPGSEEKWTLKISGYKNEKV"
     gene            3648..4358
                     /locus_tag="PLO70_11070"
     CDS             3648..4358
                     /locus_tag="PLO70_11070"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HQZ75052.1"
                     /translation="MKKIFTLFFASLFSLSLLAFEGSRLSISSLATSAEMKIEVDGRK
                     FTMKNNSITLSYLAEGYHQVKIFREKKGKGLAFGRNDVVVYSNSVFLKRGFHLDITVN
                     RFGKVLIDERRIDRDDDWYQDEDDYYDNGNGGWNDGYGNVMNGREFEVLKESLRKEWF
                     EANRLISVRFIIDKNNFTTQQVKELMLLFTFEKNRLEIARYAYSKTVDKKNYYLVNEV
                     LTFSSSKDELARFIRNSK"
     gene            complement(4457..5332)
                     /gene="sucD"
                     /locus_tag="PLO70_11075"
     CDS             complement(4457..5332)
                     /gene="sucD"
                     /locus_tag="PLO70_11075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQZ75053.1"
                     /translation="MSILINKDSKILVQGFTGTEGTFHATQMIEYGTNVIGGVTPGKG
                     GSTHLERPVFNTVADCVKETKADVSIIFVPPAFAADAIMEAADAGIKLVVCITEGIPV
                     QDMVKVKNYLQGKNTRLVGPNCPGVITAGECKVGIMPGFVFIPGRIGIVSKSGTLTYE
                     AADQVAKAGLGISTAVGIGGDPIIGTTTKEAVELFMNDTDTDAIVMIGEIGGGMEAEA
                     ANWIKSNGNKKPVVGFIAGQTAPPGRRMGHAGAIVGGADDTAAAKMKIMAECGIHVVS
                     SPADIGKKMAEVLKK"
     gene            complement(5391..6239)
                     /locus_tag="PLO70_11080"
     CDS             complement(5391..6239)
                     /locus_tag="PLO70_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ75054.1"
                     /translation="MIEVKNIKKSFDGKVVIEDVSTVMKAGQCNLIIGTSGSGKTVLQ
                     KCIVGLFEPDSGEVLYSGNNFTAMKGEEKTEIRKEIGMLFQGSALFDSLNVEDNIMFP
                     LNMFTHNTHKQKLDRVNEVLNRVNLPGVNKKFPAELSGGMKKRVALARAIVLNPKYLF
                     CDEPNSGLDPQTSLVIDKLIKEITIEFNMTTVVNTHDMNSVMEIGDYILYMYQGRKEW
                     EGSKKDIIFSKNQLLNEFIFASEFLQDAKDMRMLEETGKIPNHRNMDLMLDKDTAPLA
                     PPDQGE"
     gene            complement(6248..6985)
                     /locus_tag="PLO70_11085"
     CDS             complement(6248..6985)
                     /locus_tag="PLO70_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ75055.1"
                     /translation="MTIFNDIGRYLLMIKGMFSKPENGKMYWKEFIHQCTEIGFGSLG
                     IVAIISVFIGAVSTIQTAYQLVSPLIPPSTIAQIVRDTVILEFAPTLVCIVLAGVAGS
                     RIASELGNMRVSEQIDALEIMGINTKAYLIMPKIAAALLMIPLLVVIAMALGIWGGRL
                     AGTMSGILSGNIYDKGLLEFFVPYNVFFALTKAYVFSFIISSIPAFYGYYVKGGALEI
                     GRASTKAVVVSCILILFADYILSALLL"
     gene            complement(7060..9417)
                     /locus_tag="PLO70_11090"
     CDS             complement(7060..9417)
                     /locus_tag="PLO70_11090"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQZ75056.1"
                     /translation="MQNDLRKQQALEYHAKGRPGKIEVIPTKEAKTQRDLSLAYSPGV
                     AAPCLEIAANPEDVYKYTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGVLFKIF
                     ADIDVFDIEINEKDPEKFVQIVKALEPTFGGINLEDIKAPECFYIEQRLKAEMNIPVM
                     HDDQHGTAIISAAALLNACEVQKKKIDKAKFVVSGAGAAAVACMKLYIALGAKRENFR
                     VFDSKGLIHESRTDLDELKKEFIATGKNVTLTEGMKDADVFIGLSKGGLLNADMIKSM
                     AKNPIVFAMANPDPEISWEDATGARKDIIMATGRSDYPNQVNNVLGFPYIFRGALDVR
                     AKHINEAMKLAAVKALAELAKTPVPDIVNMAYNETNISFGEHYIIPKPLDPRLLVTVA
                     PAVAKAAMESGEAQQPITNWDEYVNNLEKRLGLDNQVMRAIGNKARRDPKRIVFAEAD
                     NVKILKSAQIIFDEGIGYPILLGDENKIRTIADTNGIDIEGLPIFDPRSDAMEDKRNQ
                     YAEIFFAKRGRKGFNAYEAKKIMKDRNYYGCMMVECGDADAMISGLSKNYPDTIRPAM
                     QIIGTEEGVSKIAGMYMMLTKKGPLFLADTTVNFNPTAEELAEITLLAAREVRYFNIV
                     PRIAMLSYSTFGSSNSPEARLVAEARKIVKAKMPSLIVDGEMQVNVAFNKELMKENYP
                     FCELVDKDVNTLIFPNLAAGNIAYNLMKELETADAVGPILLGLKKPVHILQLGSSVRS
                     ILNMALIAVVDAQIKSKTPTDEIVKRSRWWKRFRKEKPADAGAYR"
     gene            complement(9530..10708)
                     /locus_tag="PLO70_11095"
     CDS             complement(9530..10708)
                     /locus_tag="PLO70_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQZ75057.1"
                     /translation="MQEKIKALAKKYFPEFIEIRHHLHEHPELSYQEFETSKFVQNKL
                     NEFGIPFEVKATTGVVGLIKGKNPESRIIALRADMDALPIKEENDIPYKSKNEGVMHA
                     CGHDVHTTCLLGAAKILQELKDEWEGTVKLIFQPGEEKNPGGASLMIKDGVLENPKPQ
                     AILALHVNTILETGQLSFRGGKVMASADELYFTIKGKGGHAASPHLIVDPILISSHLV
                     IALQQLISRNNNPFNPSVLSITSFNGGNTTNVIPNEVKLMGTFRAMDEEWRFKSHALM
                     SKLTRDLVHSMGGEVDLHIDVGYPCVMNNEKLTADAMKKASMYMGTENVSETELRMGA
                     EDFGYYAQQIPACFYRLGTMNASKGITAGVHTPTFNIDENAIEIGMGMMAWLGSSIHT
                     "
     gene            complement(10772..11230)
                     /locus_tag="PLO70_11100"
     CDS             complement(10772..11230)
                     /locus_tag="PLO70_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQZ75058.1"
                     /translation="MKFLLTIAVFLLTNACFAQDTAWKKPIDSNSVVVHKDPRIDLLV
                     KKQAQINEETSRAGRRTDKGFRLMIISTNNRDEALAAKTKVYTYFPELKAYLWHQSPY
                     YKVKAGNFKDRKEAEAYQKRLNVYFPKGVFIMKDIIEVKSARPGEEETIL"
     gene            complement(11263..12213)
                     /locus_tag="PLO70_11105"
     CDS             complement(11263..12213)
                     /locus_tag="PLO70_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQZ75059.1"
                     /translation="MEKAIKKSSLSISENGLEKKSSAKRLEVLKTYKIYIGGQFPRTE
                     SGRYYIPTNAAGKKLANVCLSSRKDFRDAVVAARAAFGGWSGRAAFNRSQILYRMAEM
                     LEGRKSQFIDELVQQDSPKAKAEKEVNLSIDRLIYYAGWCDKFQQLFSAVNPVASSHF
                     NFSVPEPTGVVSVIAPQADGLLGLVSVIAPVIAGGNTCIILASETKPLCAVTFAEVLN
                     SSDLPGGVVNILTGKPSELASWFVDHMDVNATIYCENNSEIKKMIREKSAMNLKRITF
                     YDKVNWYTFEGQSPYFIMDTQEIKTTWHPVENIAGAGGGY"
     gene            complement(12303..13814)
                     /locus_tag="PLO70_11110"
     CDS             complement(12303..13814)
                     /locus_tag="PLO70_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQZ75060.1"
                     /translation="MAKAIAKKHVPKKSGKSNTIKLKFDTSWSYAPAPESKSAATINK
                     QYDLYINGEWQKPQSKKYFDTINPATEEKLSEVAEANAADVDKAVKAARNAYEKVWKK
                     MQAKERAKYIFRIARIMQEKARELAIIETLDGGKPIKESRDFDIPTAANHFFYYAGWA
                     DKLEYAFPNRKTEPLGVAGQIIPWNFPLLMAAWKIAPALATGNTVVLKPAETTPLTAM
                     KLAEIIQEADLPPGVVNIIAGAGTTGAAIVNHPDVNKIAFTGSTDVGKIIQRAIAGTN
                     KKATLELGGKAANIIFEDAPIDQAVEGVINGIFFNQGHVCCAGSRLYVQESVAKEVIR
                     KLKDRLESLIVGDPLDKNTDIGAINSRSQLDIIHKYISIGKKEGAEMYESSCRLPGKG
                     FFCRPTLFTNVAQSNRIAQEEIFGPVLAILTFRTDDEVIEKANNSPYGLSAGVWTDKG
                     SKIFNMTKRMRAGVVWANTFNKFDPTSPFGGYKESGFGREGGLHGLSAYVNLI"
     gene            complement(14179..15099)
                     /gene="deoC"
                     /locus_tag="PLO70_11115"
     CDS             complement(14179..15099)
                     /gene="deoC"
                     /locus_tag="PLO70_11115"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQZ75061.1"
                     /translation="MPVTKDRINLPDFNLSPRIDQVGVEERASRFTKRSIKKESKMNG
                     LKLVLNMIDLTTLEGKDTDGKVKQLCYKAMHPHDAVTDIPTVAAICVYPSMVRVAKKA
                     LVDSGIKVASVATAFPSGQAPRDIKIRDTRYAVNEGADEVDMVISRGKFHAGEYNFVF
                     DEIAAIKEACGSARLKVILETGELGTFDKVRRASDIAMLAGADFIKTSTGKIQPAATM
                     PVTLVMLEAIRDFYYKTGKMIGMKPAGGISKSKLALHYLVMVKEVLGEDWLNNEWFRF
                     GASSLANDVLMQIVKEKTGLYQSANYFSVD"
BASE COUNT         3957 a         3320 c         2917 g         5001 t
ORIGIN      
        1 actttttcat ttttataacc gctaattttt agtgtccatt tttcttcact gccgggcaat
       61 gttttatcac gataagtggt atattcaata ccaaggtctt tgttagtcca gggtacatta
      121 atcagctggt tcagctggta aaaacgattg tgtttgatga acatccagcc tgcgccatag
      181 ccaccccgat cggcttcagt aaccgggaac gagaaggttt ttttctcctg gtgaagttta
      241 agaaaggaat atttgtgaac tgatggatcc tgaatgccgg tttgattgtc cagctgctgt
      301 actacaaata aattatctgc agttgttccc agttctactt tggctgtttc gcctggttct
      361 gcagttgttt tcttgacacc ggtccacagg taatccggtc tgtttagctg cttactcttt
      421 tcatcaaaca gttcgatata cttcagatca ttaactgctt ccccgttttt atccgttgtg
      481 gcaatttcta ttatataaaa gccggggctg tattctccat tcactactga ccagtcgcca
      541 ttcattttta ctgaatcagt tttctcaaaa attttctctc ctttctccca actcttataa
      601 tcactttcat tatcatattc atcataaggg aagtttcgga tatactcgtc ttttgtcatt
      661 acaaactggt ctggtctttc ccagtagcgg tcacggatca gccttttttc ttctactaat
      721 ttatgaatag taactttcac gactgccggt tcatattcac cattcaaatt ctgggtgcgg
      781 atagacagtg tctttagact atctgcaggc agggtagatg gtatactggt tagcaggatc
      841 agtgatttat agcttacaga gacggatttc tctccgcttc ttgtttcacc gttgatgtcg
      901 gttacatcag catacaccgt ataatcaaat accggttcga atttcttttc aattttcagg
      961 tcaggtatgg cagtgaattt tacggcaaat tttccatcct tatctgtttt tacttcccca
     1021 tgcgcaattt ccataggctc accgggtggc cgccaccaac gccagaacag ccatggataa
     1081 atgaaccggg gatttcgtac caccctatat ttcactgtag ctccatcaat attgttaccg
     1141 gcataagctt ttgcaatacc ggtcacaaga atagtatcat tcactttgta agtgccttta
     1201 atcgtttcat actccacgta gaatttaggt cgtttgtatt cttctacttt aaaaccagcg
     1261 ttgccaccat ctttcctggt atagatactg aactggccat tcataccggt ttgtggcaat
     1321 tggaatttcc cattgaaaga gccgaattca tttgttttca ggttgagggt atctgcatct
     1381 ttgtaatttg catcccggag tacaaccgtt gttgaataat cagcattaac actcccggtc
     1441 ttatcttttt ggtttctgct caaaacaatt cccttgaagt aaacagtctg ccctggtctg
     1501 taaatagctc tgtcagtgaa caggtgaacg ctggtaacgg gtttggtttc caccggttgg
     1561 ttgttataat aataataata agccaggtca ttcataaata acctgtcagc ttcatgtctt
     1621 atatcaatga ggtaagagta attaccataa ttattacttt cttttttcgc tttatcgagg
     1681 ataaagaaac cgttggcatc agtagtatag cttttccctt tttctttaat gtatttggat
     1741 tgtttataat catatttctg ctcccatacc tgcaccaacg ctttggcaat tggctggcca
     1801 ttatcacggt tcagtacaaa gtaatcatta ctgttatgca catagctgat accggataca
     1861 taaaaaaacc gggcaccaat gatcgtttta ttgccatcaa gctccttttt atctgaagct
     1921 accagtatat attcgccagt ggcaagtcca tcaatcttta tttctacgct atgttcctgc
     1981 agatcgttgg tagctggcaa ttgctgctgc cagtgtttaa aaggagttgc gctaatgata
     2041 gcaggccagt atttttcatc atactggttc atcagcagtt cctttagttt ttcatcagct
     2101 ttgatgatgc gtaagtaaag ggtattgata tttttgtagt taaccaatga ccggaaagat
     2161 tgacccggaa catttacttt ttctacggtg aactgcagcg acttgctatt aatggcattc
     2221 agcaggttgt aagcattgat ctttccttca gaagaatctg tttgttgaag tactctttca
     2281 catatttctt ttgcttttat ccggtcaaac cggtaggtag tatcaccatt gggtttatag
     2341 ctgtctgctt tttcattata atattgtgcc agtaaatacc aggcttgcga agcagccggc
     2401 atattttcgt attgatgcgc tatatgatta atggcattga aatataactt gtctttatcc
     2461 gggtgtgtcg agttagtatt tacaaattca attcgcttga tatctgcatc aattagtgca
     2521 tccggctttt tatcggcaag gtgaatgttg atcagctttt gataaatcag cagtgcctta
     2581 tgttgtaagg aaagagaatc tttggttgtg aattttcttt taacaaaatc ggcagcagga
     2641 tcaaaagcac tcgcctggtc aatctcaaaa gcataagcag gcttattgat atctctttca
     2701 tcattttcaa aataactgat ggcgcggtgg gccagtaaat cgtatagggt gggacgtaga
     2761 tggcggatat ttcccctgat aatgatcgca tcaaaaggtt ccagttttgt ttgctgtaat
     2821 aatttttctt cttttatgga ttgcaggtac agctcgccga tctttttgtg aaaatctacg
     2881 gcatcccatg tggcaatatc tgttttattg aacttttctg ttttagttct gtcgtaaagc
     2941 tgccagcggt gctgctggta ataattccag tgcatttcgg ctaaaatact tttcagaata
     3001 gcagttacag gttccttgct aaggctgatc tctttttcta cttctgcaaa agaaaagatt
     3061 tcattgtcct cccggttttc tgattgcaga acagtcatgt agaccagtga tttaatgacc
     3121 tgggcatcct gcttttcctt tttggccagt acatatattt ttttcacttc gaccagagca
     3181 gacttgggaa gttccttttt gttaaaggct tccactttct tccactcttt ctcatagttc
     3241 tttatgggct gctggcttcc ggcgttcata gcgaataaac tgaataggct aacggttagg
     3301 aaaaaggttc ttacggtttg cattttcatg cttcaaaaat ttagtacaag gtaattaatc
     3361 tgatgcgggt ataagatgct ttaactcatc ataattcata atttttgttt ccctggagcc
     3421 tgtaccgcag ggaaaaatga aatattttca gtatttcatt ctttgtaggt actgaaaaac
     3481 agcagcagca acctctttac cggactgcat tcctgcatat ttccgcataa taccgttctt
     3541 cttatttttt gctttaacat ttcgttgaat caatttttgg cacgatggca tggctttgga
     3601 aataatccag ctgtaacaaa tttcaataac caattaaatt tacaatcatg aaaaaaatct
     3661 ttacactctt ttttgcttcc ttgttttcac tttccctgct ggcctttgag ggcagccgtc
     3721 tcagcatctc ttcattggct accagtgcag aaatgaaaat tgaagtggat ggcagaaaat
     3781 ttaccatgaa aaataacagt attacactca gttatcttgc agaaggatat caccaggtaa
     3841 aaattttccg cgagaagaaa ggaaaggggc ttgcctttgg ccgtaatgat gtagtagttt
     3901 acagcaactc agtgtttttg aagagaggct tccatctcga cattacggtg aaccgttttg
     3961 gcaaagtact gattgatgaa cgacgtattg accgggacga tgactggtac caggatgaag
     4021 acgattatta tgataacggc aatggtggct ggaatgatgg ctatggtaat gtaatgaatg
     4081 gccgtgagtt tgaagtatta aaggaatcac ttcgtaagga atggtttgaa gcaaacaggt
     4141 tgatttctgt aaggtttatt attgataaga ataacttcac cacgcagcag gttaaagagc
     4201 tcatgttatt attcactttt gaaaaaaaca ggctggaaat tgccaggtat gcttattcaa
     4261 aaacagttga taagaaaaat tattacctgg ttaacgaagt gctgacattc agcagcagca
     4321 aagatgaact ggcccgtttt atccgtaaca gcaagtaaga ttataggaat ggtaattggt
     4381 gagaaagaaa ttctctccaa ataaaaagcc ctctgcataa tgcagagggc tttttaatct
     4441 attttctatt caccaattat tttttcaaca cttcagccat tttctttccg atatctgccg
     4501 ggctgcttac cacatgaata ccgcattcag ccatgatttt cattttagca gcagcagtat
     4561 catctgctcc cccaacgata gcaccagcat gtcccatacg gcgaccagga ggggctgttt
     4621 gaccggcgat aaagccaaca accggttttt tatttccatt tgatttgatc cagtttgccg
     4681 cttctgcttc cataccgcca ccaatttcac caatcataac gatggcatct gtatcggtat
     4741 cattcataaa taattcaaca gcttctttgg tcgtagtacc aatgatcggg tcaccaccta
     4801 ttccaacagc agtagaaatg cccaaacctg ctttggctac ctggtcggca gcttcatacg
     4861 taagcgtacc tgattttgat acaataccta tcctgcccgg aatgaaaacg aatcccggca
     4921 tgatgccaac tttacattct ccggctgtta taacgccggg gcagttaggg cctaccagtc
     4981 ttgtattttt cccctgcagg taatttttca cttttaccat gtcctgtaca ggtatgcctt
     5041 cagtaataca gacaacgagt ttgatacctg cgtctgcggc ttccatgatt gcatccgcag
     5101 cgaaagccgg aggcacaaaa atgatactaa catctgcttt ggtctccttt acgcagtctg
     5161 ctacagtatt gaagactggc ctttccaaat gcgtgctgcc acctttacca ggggtaacgc
     5221 caccaataac attggtgccg tattcaatca tttgtgtagc gtgaaaagta ccttcagtac
     5281 ctgtaaaacc ctgtacgagg attttagaat ctttattgat aagaatactc atattatgtt
     5341 cttaaaagtt gggcaaaagt aaggtattga cgctttataa taaagaggca ttattccccc
     5401 tggtcaggag gggccaatgg cgctgtgtcc ttatcaagca taaggtccat attccggtgg
     5461 ttgggaattt tacctgtttc ttccagcatc cgcatgtctt tggcatcctg caggaattcg
     5521 gaagcgaaaa taaattcgtt tagcagctgg tttttgctga atatgatatc ttttttagag
     5581 ccttcccatt ctttccggcc ctggtacatg tataaaatat agtcacctat ttccatcaca
     5641 ctattcatgt catgggtatt taccacagta gtcatattaa actctatggt tatttctttg
     5701 atcagtttgt caattaccag cgatgtttgg ggatccaggc ctgaatttgg ttcatcacaa
     5761 aaaagatatt taggattaag cacaattgct ctggcaaggg caactctttt tttcattccg
     5821 ccacttaatt cagccggaaa ttttttattt acgccaggga gattaacccg gttcagcact
     5881 tcatttaccc gatcgagctt ttgtttatgt gtattatgtg taaacatgtt tagcgggaac
     5941 atgatattat cttccacatt caggctgtca aaaagagcag atccctggaa aagcatgcct
     6001 atttctttcc tgatctcggt tttttcttcc cctttcatcg ctgtgaaatt attcccgctg
     6061 tataaaactt caccactgtc tggttcaaat aaccctacga tgcatttttg taatactgtc
     6121 tttccgctgc cactggttcc aataattagg ttacactgcc cggccttcat cacagtgctt
     6181 acgtcttcga tgacgacttt tccgtcaaag ctctttttta tgttttttac ttcaatcatt
     6241 caaccggtta aaggagtaat gctgataaaa tataatccgc aaacaaaata agtatacaac
     6301 ttactacaac agcttttgta ctggccctgc caatctccaa tgcaccccct tttacataat
     6361 aaccataaaa agccggtata ctggagataa tgaaggagaa tacataagct ttggtaaggg
     6421 caaaaaatac attatagggt acaaagaatt caaggagccc tttatcataa atgttcccgg
     6481 aaagaattcc ggacatggtt ccggcaagcc ttccccccca gatgcccaat gccattgcga
     6541 ttactaccag caaagggatc attaataagg cggcagcaat tttaggcatt attaaatagg
     6601 ctttggtatt tatgcccatg atttcgagtg catctatctg ttcacttacc cgcatatttc
     6661 ccaactcact ggcaatccgg ctacccgcta cccctgctaa tacaatacat acaagtgtag
     6721 gcgcaaattc aagaattacg gtatccctta caatttgagc aatggtagag ggggggatca
     6781 gtgggctaac caactggtag gcagtctgga ttgtagaaac ggctccaatg aatacagaga
     6841 tgatggctac aatgcccagt gaaccaaaac cgatctcagt acattgatga ataaactctt
     6901 tccaatacat cttaccgttt tccggtttgg aaaacattcc ttttatcatc aaaaggtaac
     6961 ggcctatgtc gttgaaaatg gtcatttgag ttgctaagat acaggatgaa aagagaaaag
     7021 ccagtagcgg ggctattggc ttttcgatta agtattactt taacggtaag ctcctgcatc
     7081 tgccggtttt tccttacgaa agcgtttcca ccaacgggaa cgtttgacta tttcatccgt
     7141 aggggttttg cttttgatct gtgcatctac cacagcaatt aaagccatat tcaggatgct
     7201 gcgaaccgag ctccccagtt gaagaatatg taccggtttt ttcaatccca acagaattgg
     7261 tcccactgca tccgccgtct ccagttcttt catcaggttg taagctatat tgcctgctgc
     7321 caggttagga aaaattaatg tgtttacatc tttatccacc aactcacaga atggatagtt
     7381 ttctttcatt aattctttat tgaacgccac atttacctgc atctcgccat caactattaa
     7441 tgaaggcatt tttgctttta caatttttct tgcttcagca actaatcttg cttcgggtga
     7501 attactgctg ccaaaagtgg agtaactcag catggcgata cggggaacaa tattgaaata
     7561 acgtacttcc ctggctgcta aaagggttat ttcagccagt tcttccgccg tggggttgaa
     7621 gttgactgta gtgtctgcca ggaaaagggg tccttttttg gtcagcatca tgtacatgcc
     7681 tgcgatcttg ctaacacctt cctcggtacc gattatttgc atagccgggc ggatggtatc
     7741 aggatagttc ttggaaaggc cggagatcat ggcatctgca tcaccgcatt ccaccatcat
     7801 acaaccatag taattgcggt ccttcattat tttttttgct tcataggcat taaaaccttt
     7861 tcttcctctt ttggcaaaaa atatttctgc atactggttc cttttatcct ccatcgcatc
     7921 acttctcgga tcaaaaatgg gcaaaccctc aatgtcgatc ccgttagtat cagcaattgt
     7981 tcttatttta ttttcatctc ccagcaggat agggtagcca atgccttcat caaagatgat
     8041 ttgtgctgat ttaagaattt ttacattgtc agcctccgca aaaacaattc gttttggatc
     8101 acgacgggct ttattaccaa tggccctcat cacctggtta tccagtccca atcttttttc
     8161 caggttattt acatattcgt cccagttagt aatcggttgc tgcgcttcac cactttccat
     8221 ggcagccttt gcaactgcgg gtgctacagt gaccaacaat cttgggtcta acggtttggg
     8281 aataatataa tgttcaccaa aactgatatt ggtttcattg taggccatat tcacaatgtc
     8341 aggaacaggt gttttggcca attcggccaa tgcttttaca gcagccagtt tcatggcttc
     8401 attgatatgt tttgcacgaa catcaagtgc accgcggaaa atgtaaggga aaccgagtac
     8461 attatttacc tggttgggat aatcgctgcg gccggtggcc attatgatat cctttctggc
     8521 accggtagca tcttcccagc ttatttcagg atcagggttg gccattgcaa aaacgatggg
     8581 attttttgcc atgcttttga tcatgtcagc atttaacaag ccccccttac ttaacccaat
     8641 aaaaacatcc gcatctttca taccctctgt aagggttaca tttttgcctg ttgctataaa
     8701 ttcttttttt agttcatcca ggtcagtacg gctttcatga ataaggccct tgctatcaaa
     8761 aaccctgaaa ttctctcttt ttgctcccaa ggcaatatat aatttcatac aggcaacagc
     8821 tgctgcgccg gcaccactta ctacaaattt tgctttgtca atttttttct tttgcacttc
     8881 gcaggcattc aataaggcgg cagcactgat tatagcggta ccatgctggt cgtcatgcat
     8941 taccggaata ttcatctccg cttttaatct ttgttcaatg tagaaacatt caggtgcttt
     9001 aatatcttca aggttgatgc ctccaaaagt tggttccagg gcttttacaa tctgtacaaa
     9061 tttttcggga tctttttcat tgatctcaat atcgaaaaca tcaatgtctg caaatatttt
     9121 gaaaagtact cctttgcctt ccattaccgg cttaccagct tccgggccga tatcacctaa
     9181 acctaacact gcagttccgt tactgatgac tgcaaccagg tttcctttgg ccgtgtattt
     9241 gtaaacatct tcagggttag ccgctatttc gagacagggt gcagcaacac cgggtgagta
     9301 agccagagaa aggtctcgtt gagttttggc ttctttggtt ggtattactt caatctttcc
     9361 gggtcttcct ttggcgtggt attcaagagc ctgttgtttg cgtaggtcgt tttgcataaa
     9421 tctgttttaa ttctgatcat tcatcacatg attttcatac ggaagtgcaa ggtacaaaat
     9481 aaaaacccgg tgaaatttta aagaaaaggt ccttaattaa agaagttgtt taagtgtgga
     9541 tactactacc cagccatgcc atcattccca ttccaatttc aattgcattt tcatcaatat
     9601 taaaggtagg agtgtgaacc cctgctgtaa tgccttttga agcattcata gtacccaggc
     9661 gataaaaaca ggccggtatt tgttgtgcat aatagccaaa atcttcagca cccatccgga
     9721 gttctgtttc gcttacgttt tctgtaccca tgtacattga agcttttttc attgcatcag
     9781 cagtaagttt ttcattattc attacacagg gataaccaac atcgatgtgc agatcaactt
     9841 ctcctcccat tgaatgcaca agatctcttg ttaatttact catgagggca tgtgatttga
     9901 aacgccattc ttcatccatg gccctgaatg ttcccatcaa ctttacttca ttgggaataa
     9961 cattggtggt atttcctcca ttaaaagaag taatggaaag aacggatgga ttaaaaggat
    10021 tattatttct actgatcaac tgttgtaaag caatgaccag gtgagaactg ataaggatcg
    10081 ggtctactat cagatgcgga gaggcggcgt ggcctccttt tccttttatt gtaaaatata
    10141 attcatcggc actggccatt acttttccac cacgaaaact caactgacct gtttccagga
    10201 tggtgtttac atgcaaggca agtatagcct gcggttttgg gttttccagt acaccatctt
    10261 ttatcatcag cgaagcccca ccgggatttt tttcctctcc gggttgaaat attaatttta
    10321 ctgtgccttc ccattcgtct ttcaattcct gcaggatctt tgcagcaccc agtaggcagg
    10381 tggtgtgcac atcatgtccg caggcatgca tcactccttc gttctttgat ttgtagggga
    10441 tatcattttc ctctttgata gggagggcat ccatatcagc acgaagtgca attattcttg
    10501 attcgggatt tttgcctttt atcaaaccga caacacccgt tgttgctttt acttcgaaag
    10561 gaattccaaa ttcattcagt ttgttttgaa caaactttga agtttcaaat tcctggtaac
    10621 ttaattccgg atgttcatga agatgatgac ggatctctat aaattcagga aaatattttt
    10681 ttgcgagggc ttttattttt tcttgcatac agcaaaaata aacaaaccat tctgtgtaag
    10741 aatggtttgt cagctaagta gttttattct tttacaggat tgtttcttct tctcccggtc
    10801 tggctgattt tacttcaatg atatctttca taataaagac gcctttcggg aagtaaacat
    10861 tcagtctttt ctggtaggct tctgcttctt tccggtcttt gaagttgccg gctttcactt
    10921 tgtaataagg cgattggtgc cacaggtaag ctttcaattc agggaagtaa gtatatactt
    10981 ttgttttggc agccaacgct tcatcccggt tattggtact gatgatcatc aggcgaaaac
    11041 ctttatctgt tcttcttccg gcccggctgg tttcttcgtt gatctgggcc tgttttttca
    11101 ctaagaggtc aatacgggga tctttatgaa ccacaacaga attactgtct ataggctttt
    11161 tccacgctgt atcttgtgca aaacaggcat ttgttaacaa aaagacggca attgtaagta
    11221 ggaatttcat gtgtggcata aggtgcaaat atcttaccaa aattagtatc ctccgcctgc
    11281 accggcaata ttctcaaccg ggtgccaggt agtttttatt tcctgggtat ccattatgaa
    11341 gtaaggagac tggccctcaa aggtgtacca gtttacttta tcatagaaag taatgcgttt
    11401 cagattcata gcagattttt cccttatcat tttctttatc tcagaattgt tttcacaata
    11461 aatggttgca tttacatcca tatgatcaac gaaccaggaa gccagttcgg atggttttcc
    11521 tgttaaaata ttgacaacac cacccggcag atcagaggaa ttaagcactt ccgcaaatgt
    11581 aacggcacag aggggttttg tttctgatgc cagtatgata caagtattac caccggcaat
    11641 tacaggtgca atcacggata ccaaacctaa taatccatct gcctgcggag cgatgacaga
    11701 tacaactcct gttggttccg gaacagaaaa attgaaatgg gaacttgcta cagggtttac
    11761 agcgctgaat agttgttgaa acttatcaca ccatccggca taatagatga gccggtcaat
    11821 agacaggttg acttcttttt ctgcttttgc tttcggggaa tcctgttgta ccaactcatc
    11881 aataaattgt gatttccttc cttccagcat ttctgccatg cggtataata tctggctgcg
    11941 gttgaaagct gcacggccac tccagccacc aaaggcagct cttgcagcca ctactgcatc
    12001 cctgaaatcc tttcgggaac tgaggcaaac attggccagt ttctttcctg ctgcattggt
    12061 tgggatatag taacggcccg attctgttct ggggaattgt ccgccgatat aaattttata
    12121 ggtcttcagc acttccagtc ttttggcgga agattttttt tctaatccgt tctcactgat
    12181 actgagcgaa gattttttaa ttgctttttc catatgatag aacgcagatt tttatgatag
    12241 ttatgattgt cgctggttat cataattaat cttattaatc ataataatca gcgttcttgt
    12301 atttaaataa gatttacata agcagaaagg ccatgtaacc cgccttccct tccaaatcca
    12361 ctttctttat agccgccaaa tggtgatgtt ggatcaaact tattgaacgt atttgcccaa
    12421 accacaccag ctctcattct ttttgtcata ttaaagatct tggagccttt atcagtccac
    12481 acaccggcac ttagcccata tggtgaatta ttagctttct caattacttc atcatctgtc
    12541 cggaaggtaa ggatggctaa caccggtccg aatatttctt cctgcgctat acggttactt
    12601 tgtgcaacat tggtaaacaa agtaggacgg cagaaaaatc ctttacccgg cagtcggcag
    12661 ctgctttcat acatttcagc accttctttt ttaccaatac tgatgtattt atgaataata
    12721 tctaattgtg acctggaatt gatggcgcca atgtcagtat tcttatccaa tggatcacca
    12781 acaatcaatg actccaaccg gtcttttaat tttctgatga cttctttggc cacagattcc
    12841 tgtacatata atcttgatcc ggcacagcaa acatgtccct ggttaaagaa tataccattg
    12901 ataacacctt ctactgcctg gtcaataggc gcatcttcaa aaatgatatt ggcagcttta
    12961 ccacccaatt ccagcgttgc ttttttattg gttccggcaa tggcccgttg aattattttt
    13021 ccaacatcag tagaaccggt aaaggcaatt ttattaacgt cagggtgatt aacgatagcc
    13081 gcacctgttg ttcctgcacc cgcaataata tttactacac ccggtggtaa atcagcttcc
    13141 tgtattattt ccgccagttt cattgcggtc aatggcgttg tttctgccgg tttcaagaca
    13201 actgtatttc ctgttgccag ggccggtgca atcttccatg ccgccatcaa taatggaaag
    13261 ttccagggaa taatttgccc tgctacgccc agtggttcag ttttcctgtt gggaaaagca
    13321 tattccagtt tatcagccca gccggcataa taaaagaaat ggttggcagc ggttggtata
    13381 tcaaagtcac gactttcctt aatgggtttg ccgccatcaa gtgtttcaat gatcgccagt
    13441 tccctggctt tttcctgcat tatcctggcg atgcggaaaa tatatttagc cctttctttt
    13501 gcctgcattt tcttccacac tttttcatac gcatttcttg cggcctttac cgctttgtcc
    13561 acatctgctg cattcgcttc tgccacttcg ctcagctttt cttccgttgc cggatttatg
    13621 gtgtcaaaat attttttgct ctgtggtttt tgccattccc cgttaatata caagtcgtat
    13681 tgtttattaa tcgttgctgc gcttttgctt tcgggtgcag gtgcatagct ccatgatgta
    13741 tcaaatttta atttgatcgt attgcttttc cctgattttt tcgggacatg cttttttgca
    13801 attgcttttg ccatgtaaaa aattaattta tgttatcagc gacattaaca cggatgaact
    13861 tccgttgcgt cgcactcttc atcgttttat tcgcttatca tcaaaaaaaa tcccacaggg
    13921 ttcacttacc gggtatttgt tacctttctc ttgtttttta attttataac acagtcaatc
    13981 aaataagctg agaacagaaa aaactgaacc cacctcatcc atcttttctc cggtacttcc
    14041 tcccaaaatt tataatatat catgagggtt aagtatgtgg ataggacaat tatttgaatc
    14101 cataatccat atttccccca gatttttttc agctttaaat ttttgcgcct ttgtttgctg
    14161 gcgtttttgt gagaaaatct aatctaccga aaaataattt gcactctggt acaatcctgt
    14221 cttctctttc acaatctgca tcaatacatc attggcgagg ctgctggcac caaaacggaa
    14281 ccattcatta ttcagccagt cttcacccag cacttctttt accataacga gataatgcaa
    14341 agccagtttg gatttggaaa ttccaccggc tggcttcatt ccaatcattt tacctgtctt
    14401 ataataaaaa tctctaatcg cttccagcat tactaatgta accggcatag tggcggcagg
    14461 ttgtattttc ccggtggatg ttttaataaa gtcagcccct gcaagcatag cgatatcact
    14521 ggcccggcga accttgtcaa aagttccgag ttcgcctgtt tcaaggatca ctttcaatct
    14581 tgcactgcca caggcttctt ttatagccgc tatttcatca aatacaaaat tatattcacc
    14641 ggcatggaat tttccgcggc tgataaccat atccacttca tctgctcctt catttaccgc
    14701 atacctggta tctcttattt taatgtctct cggcgcttgc ccgctgggga aagcagtggc
    14761 tacagaagct acttttatac ctgaatcaac caatgctttt ttcgccactc tcaccatcga
    14821 cggatataca caaatcgccg ccacagttgg aatatccgtc accgcatcat gcggatgcat
    14881 ggctttataa caaagctgtt tcacttttcc gtcagtgtct tttccttcca gggtggtcag
    14941 gtcaatcatg ttcagcacca gcttgagccc attcatcttg gattctttct taatgcttcg
    15001 cttggtgaag cgggaagctc tttcttccac acctacctgg tcaatccgcg gtgaaaggtt
    15061 aaagtcgggg agattaattc tatcctttgt taccggcata attctgtttt cttgatttca
    15121 aaagtaaaac tatttgcggg gattaaaact gtgtaaataa tatgtacagc ctgctgaaag
    15181 gtaatatgat tttca
//
LOCUS       DAORTI010000085        15087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3737_length_15087_cov_6.923763, whole genome shotgun sequence.
ACCESSION   DAORTI010000085 DAORTI010000000
VERSION     DAORTI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_15087_cov_6.923763"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1231
                     /locus_tag="PLO70_11120"
     CDS             74..1231
                     /locus_tag="PLO70_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQZ75062.1"
                     /translation="MPKLIRITTAPISLNVLLRGQMRYMNENGFEVVMVSSDGKELET
                     VKANEQCRHHIIPMTRRMTPLGDLRCLWLLYRFFKKEKPDIVHSHTPKAGLLAMLAAK
                     FAGVKLRIHTIAGLRFVTTKGFGRKVLIAMEKLTGKAATHVWPNSYSMEQYIIENKLV
                     SPKKLEVIGHGSSNGVSLSRYSIPALSKEKIEETKRRMNYDPSLKYFLSVGRIVHDKG
                     MDELLNAFVRLHATNPHTRLVLVGAFEDEVDPVSDKARQLIQTHPAVIMAGWSDAVEY
                     FMHLSFTLVHPSHREGFPNVLLQAGAMGCPIICSRIDGNIDIVEHATTGLLFEVKNEE
                     ELFQQMQYALDNPAVLQQYAKTLRLHIEQHFDQPVLHSLLRKKYLELLAAQ"
     gene            1361..2020
                     /locus_tag="PLO70_11125"
     CDS             1361..2020
                     /locus_tag="PLO70_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HQZ75063.1"
                     /translation="MKTTLKKPVAIVGYSGHAFVIIDILLSAGRLVTAYCDQEAKEFN
                     PYHLEYLGKESDVINKLKKFDFFACVGHNGIREKIHTNLSQYLGNPINAIHPSAVISS
                     SVKMGDGIMIAANATLNPLMEIGRGVICNTSTSIDHECIIGDFTHIAPGAVLCGNVKV
                     GRNTFIGANSVIRQGIIIGDNVTIGAGTVVVKDIPDGATVVGNPAKNLVKRPARKLVA
                     A"
     gene            2188..2871
                     /locus_tag="PLO70_11130"
     CDS             2188..2871
                     /locus_tag="PLO70_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HQZ75064.1"
                     /translation="MKPVNPKDFSILFLCKKDNEYAQRAAAFVREHFTDHLIFEGGRK
                     DKLPPEVLNWRGDLLISFISSWIYPQPLLENALIAAINFHPGSPEYPGTGCTNFAIYN
                     SEKEYGITCHHMNATVDSGSIIQVKRFPIKEDDTVYSVTQQCYKLIEESFYEVMDCIL
                     NNKSLPVTNEQWKRKPYTRKQLDELCTITPEMPEEEIERRIKATTYKTPWAFTKIGNH
                     IFKLQAENK"
     gene            2871..4385
                     /locus_tag="PLO70_11135"
     CDS             2871..4385
                     /locus_tag="PLO70_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQZ75065.1"
                     /translation="MTTATINRISKSPVTGKEYEIPSVLDDKAGIDAFIALHPGKKVV
                     VVQGLGFVGSVMGLVVANALTEEYAVIGIDLPTPASYWKIRSINEGVFPIIASDPKID
                     LYYQQALTKKNYYATYDSHAYSKADVVVVDINLDVQKKSAKNNDLEGYDVDLTPFKKA
                     IEAIGSNCKEEVLVLVETTVPPGTAQKIVRPLLEECLTKRGLTTDKLKVGHSYERVMP
                     GPKYIDSIQNFYRVFAGTDEKSAEAVERFLRTVIRTDEYPLTRLGNTNATEMAKVLEN
                     SFRAMNIAFMVEWSRFAEEAGVDIYEVVNAIRMRPTHKNIMLPGLGVGGYCLTKDPLL
                     ASWARMNLFGSEEKLGQSEKGVHINDKMPLYAFEYLQSQYTGSLAGKKVLLLGVSYLN
                     DVGDTRYTPVEGFYDQLINAGCEITLHDPHVKYWEEKDIWINQNLEELLEDECDVVVV
                     TTGHKDYRSNESLINTLLSKPAAFMYDTIGVWTKDEIKALGEKHMVKVLGRGDL"
     gene            4697..5692
                     /locus_tag="PLO70_11140"
     CDS             4697..5692
                     /locus_tag="PLO70_11140"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ75066.1"
                     /translation="MSKNVLLLGGAGFIGFNIARYLAENRDYKLTIADNFARGKQDDY
                     FTELVNKHNIRVVAGDFTDPVTFNQLDEAYDQVYMLASVVGVDNANSIPHEIIRINTA
                     LIFNTLEWVRRSKVGKVLFTSTSECYAGTIEAFGYVTPTPEEVPLCIQDIGHPRFTYA
                     VTKMLGESGFLNYARILGFETTIVRYHNVYGPRMGFKHVIPHLVVRYRKGEEPFKVYG
                     HDQTRAFCYITDAVEGTVLAMEQPNTNGEIYHIGTQDEITIGELIHYAGELMNFKGTY
                     ENAPTYPGSVSRRCPDITKARTQLGFEPKVMWKEGLKNTVEWYNQYLESGKLVYE"
     gene            5685..6833
                     /locus_tag="PLO70_11145"
     CDS             5685..6833
                     /locus_tag="PLO70_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HQZ75067.1"
                     /translation="MSNNRFIPLSVPNIAGNEWKYVKDCLDTGWISSVGSYVTQFEEM
                     VAGFAGAKYGVAAVNGTAALHISLLLSGVKRDDYVILPNLTFVASANSIKYLGAEPLL
                     IDADPDLWQMDLDLLEEFLENETDEKNGELIYIKDGRRIGAIMPVHILGNMCDMDRFL
                     AIVKKYPLPIVEDSTEALGTTYKGKSAGSFSPLACFSFNGNKIISTGGGGVIVTDDET
                     LAKHAKHLTTTAKASPDEYYHDEVGYNYRLVNVLAAIGVGQMELLPSFIKRKRECVAF
                     YKKELAGIGDIRFQKELPDVETNGWLFTIQTDKQIQLLDHLNANKILSRRFWMPMNKL
                     PMYRDCVYVQKKDTSDYIYNTCLSIPSSTSITDDELAIVIREIKAAIG"
     gene            6839..7501
                     /locus_tag="PLO70_11150"
     CDS             6839..7501
                     /locus_tag="PLO70_11150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQZ75068.1"
                     /translation="MYQIIKRFFDIVFSLVAIIILLPVFIPVILLLLLTGEHEVFYKQ
                     DRVGYKNRIFKILKFATMLKNSPNMGDGDITKRGDPRITKVGKLLRQSKLNELPQVFN
                     IFLGDMSFVGPRPLMKVGFDRYSDEMKSKVYNAQPGLTGIGSIVFRDEELIITQSNLP
                     PHDCYRDIILPYKGALEVWYKQHQNFFTDFMILFLTAWYIVFPKSNMVYKVFPSLPKR
                     SF"
     gene            7551..8303
                     /locus_tag="PLO70_11155"
     CDS             7551..8303
                     /locus_tag="PLO70_11155"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HQZ75069.1"
                     /translation="MFKLFSKSKKNESAEIAEFGLLKTDMHSHLIPGIDDGAPDIETS
                     LKLIGGLHSLGYKKIITTPHIMWDMYKNTREIILERLEWLRTTVKEAELDIEIDAAAE
                     YFLDDHVAGLLANNEPLLTVSGNMVLVEFSMAYPSHSLKGILFDMQMQGYQPIIAHPE
                     RYIYLDQNKGFYEELKDIGCLFQLNILSLTGYYGKSVQELAQYLMKKEYYDLVGTDLH
                     HFGHLEALSTRPVLAAQLKKLLDSGKIKNFEL"
     gene            8382..9260
                     /locus_tag="PLO70_11160"
     CDS             8382..9260
                     /locus_tag="PLO70_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQZ75070.1"
                     /translation="MNAFTIKDLENLSGIKAHTIRIWEQRYNFLKPQRTDTNIRYYSS
                     DELKTVLNIALLNKYGFKISHIDRMQPEEIKAKILSLGDAKAIQERIVNELVQEMVDL
                     NIEKLEKILANYISLRGIEKTVIHIIFPFLEKIGLLWVTGHINPAQEHLVTNVIRQKL
                     IVAIETAITHLKVDKTVLLFLPEGEHHELGLLFMYYMLKNRGIQTIYLGANVPVKDVA
                     YVARLKKPDIVFTHLTATSSSFNFEKFLNSINSQFNDIQTVISGQLTHSYTKNIPNGI
                     KFKKSLQEVMEYLTSL"
     gene            9390..9890
                     /locus_tag="PLO70_11165"
     CDS             9390..9890
                     /locus_tag="PLO70_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ75071.1"
                     /translation="MSSVEFNQMLLSNADFLKPFAVNLTRDTEAANDLYQETLYKALA
                     NQDKYNSGTNIKAWLFTIMRNIFINNYRRKAKQKTIFDSTPNDYLINQKQATVSNAAE
                     SDMRIKEIKNAIQQLPEIFKTPFLLYFDGYKYNEIADVLNEPLGTIKSRIHFARKLLK
                     EQISRF"
     gene            9901..11376
                     /gene="crtI"
                     /locus_tag="PLO70_11170"
     CDS             9901..11376
                     /gene="crtI"
                     /locus_tag="PLO70_11170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HQZ75072.1"
                     /translation="MSDKSVIVIGSGFAGLSAASFMASAGWKVTVIEKNSTPGGRARQ
                     LKEDGFTFDMGPSFYWMPDVFERYFAQFGKKVSDYYSLHRLDPSYRIYWNDGYTDMPA
                     DLFELKKVFESIEPGSAEKLEKYLKGAAYKYEVGVNKLVYKPSRSLTEFMDWQTISGV
                     FKLEVFTSIKKHIEKYFKHPKLRQMMEFPVLFLGALPEDTPALYSLMNYADIKGGTWY
                     PQGGMYAIVEGMYQLAMELGVTFHFEESVQEITIDDSIAKKVVTDKNSYEADAVISGA
                     DYHFTEKNLLAAQYRSYSDQYWDKKVMAPSCLLYYVGLNKKLKQPIHHSLFFDVPFEQ
                     HGEEIYKNPKWPAEPLFYVSVSSLTDDSVAPEGHENIVFLIPVASGLENDTEELREKY
                     FQVIARRMEKHIGESILDSVVYKKTYSVSDFVTDYNSFRGNAYGLANTLLQTAIFRPS
                     CQSKKVKNLFYTGQLTVPGPGVPPSLISGEVVAKQVIKAFK"
     gene            11393..12229
                     /locus_tag="PLO70_11175"
     CDS             11393..12229
                     /locus_tag="PLO70_11175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQZ75073.1"
                     /translation="MVQLFHTTSENCSRIITENYSTSFSSAIRMLHKDLRTPIFNIYG
                     FVRFADEIVDTFHDYDKAALLEQFKKETYDAIQRGISLNPALNSFQRTVNEYNIDLKL
                     VDAFFHSMEMDLSKQVYDCEGYKEYIYGSAEVVGLMCLYVFCEGEKSLYNKLEFSAKA
                     LGAAFQKVNFLRDIKADYNGLSRIYFPGCDFANFSESEKKAIEQDIQNDFEAAYEGIK
                     MLPLKARFGVYVAYKYYLSLFKKIKKLEPERVLDTRIRIPNYLKAMIVLRAGVKNQLR
                     LI"
     gene            12231..12770
                     /gene="idi"
                     /locus_tag="PLO70_11180"
     CDS             12231..12770
                     /gene="idi"
                     /locus_tag="PLO70_11180"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HQZ75074.1"
                     /translation="MDLTQVILVDQQDNPTGSCEKLEAHQKGLLHRAFSVFIFNKKGE
                     MLLQQRAVNKYHSGGLWTNACCSHPAEGEDTIAAAHRRLVEEMGFDTPVEKVFDFVYK
                     ADFDNGLTEYEFDHVFVGEYSGAINFNKEEVMDYCYKTVQAIGLSLQSHPQKYTAWFH
                     LAFPKIENWWKKHFNNQTT"
     gene            12792..13301
                     /locus_tag="PLO70_11185"
     CDS             12792..13301
                     /locus_tag="PLO70_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQZ75075.1"
                     /translation="MDILLYILVLLGTFIVMEGVTWCTHKFVMHGFLWYLHKDHHKVQ
                     PGIFEKNDAFSIIFAVPSILLIYFGTYNDVWWQQAIGFGIMAYGFAYFMVHDVIIHQR
                     FKWFTKSKSTYVRAIRWAHKMHHKHIDKHDGESFGFLFVNKKYWDKVRRDDKLMAGSK
                     KVEYAPEAE"
     gene            13370..14488
                     /locus_tag="PLO70_11190"
     CDS             13370..14488
                     /locus_tag="PLO70_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HQZ75076.1"
                     /translation="MFCGAGCASLSLLMRMIRSGKFTDKKILLLDKEPKTMNDRTWCF
                     WERENGFFDEIVYRKWDTISFFGDNFSSPMDIAPYQYKMIRGIDFYGYCFAAIKKQEN
                     IEIRYAAIGEWFMQEEEATFILDGLPYSVGRPTVFNSIYEPAKKNENIIRLLQHFKGW
                     VIETDRPFFHPEQATMMDFRVSQQHGTTFAYVLPFSATTALVEYTLFTKNLLEPHQYD
                     DGLKEYIHSFLEISNYTIKEEEFGVIPMTNEKHSFDGHGWQIGTAGGQTKASSGYTFQ
                     FIQKQSQQIVECLLSGRSLALIPATPKRFRFYDNTLLDILYNDTLPGKQIFTQLFKKN
                     KPQQVLRFLDNESSLKEELKIIATLPTWPFFKAALKQL"
     gene            complement(14489..14926)
                     /locus_tag="PLO70_11195"
     CDS             complement(14489..14926)
                     /locus_tag="PLO70_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75077.1"
                     /translation="MATTSHKPSTFLLFPWGLLSIVLVLGLNLTLDNEWFSKNWSWIA
                     LVLIIPAAILENFKVGYSTMRIMFMKVVYSLISFLLVGMAFGSALSGWHIEKFQKIPG
                     VKTITLPWYVMLALAVLLLIQVAQLFSMLKAVKREFMQMDPDN"
     gene            complement(15008..15079)
                     /locus_tag="PLO70_11200"
     tRNA            complement(15008..15079)
                     /locus_tag="PLO70_11200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15044..15046),aa:Arg,seq:ccg)
BASE COUNT         4696 a         2681 c         3337 g         4373 t
ORIGIN      
        1 gatgaaaaaa gagctaagat gctctggaca ttatttgcgt tggaagtgtg gtaccgaaaa
       61 tgttatttaa aaaatgccta aactgatccg tatcaccacc gctccgatct cactcaatgt
      121 tttactgagg gggcagatgc gttacatgaa tgaaaatggt tttgaggtag tgatggtcag
      181 cagtgatgga aaggaattag agactgttaa agccaacgaa caatgccgcc atcatattat
      241 cccgatgaca cggagaatga cacccttggg cgacctgcgt tgtttatggc tgttgtaccg
      301 ttttttcaaa aaagaaaaac ctgatattgt tcactctcat actcccaaag cgggcctgct
      361 ggcgatgctg gcagcaaagt ttgcaggagt taagctacgt attcatacca ttgcggggtt
      421 gcgttttgta actaccaaag ggtttggccg taaagtgctg attgcaatgg aaaagctgac
      481 cggcaaagca gctacacatg tatggcccaa cagctattcg atggagcaat acattataga
      541 aaataaattg gtcagcccga agaaactgga agtgattggc catggctcca gcaacggggt
      601 gagcttatcc cgttattcaa taccggccct ctcaaaagaa aaaatcgagg aaacaaagag
      661 gcggatgaac tatgatccgt cactgaaata ttttttatca gttggccgca tcgtgcacga
      721 caaaggaatg gatgaactgc ttaatgcttt tgtcagatta catgcaacaa atcctcatac
      781 ccgactggta ttggttggcg cttttgaaga tgaagtggat cctgttagcg ataaggcccg
      841 tcaattgata caaactcatc cggccgttat catggccggc tggagtgatg cagtagaata
      901 ttttatgcat ttatcattta cactggttca tccttcccac cgggaaggtt ttcctaatgt
      961 attattacag gccggtgcga tgggttgtcc cattatctgt tcccgtattg atgggaatat
     1021 tgatatagtg gaacatgcaa caacaggatt actattcgaa gtaaaaaatg aagaagagct
     1081 ttttcaacaa atgcaatatg cgttagataa cccggctgtt ttacagcagt atgcaaagac
     1141 cctgcgtctt catattgagc agcattttga ccagccagtc cttcactccc tcctccgcaa
     1201 aaaatacctc gaactgcttg cggcacaata atttcccgcc atttatcgga aatctacgag
     1261 tttcgtaaaa gtactagtac ccgatttatc ttaatagatc gttcattgtt ttggaaaccc
     1321 tggatgtgtt tttattttag ctcatccaaa atccaaatca atgaaaacca cattgaaaaa
     1381 acccgtggcc attgtcggct attccggcca cgcctttgtt atcatcgaca tcctcttaag
     1441 tgcgggtcgc ctcgttacag cttattgcga ccaggaagca aaagaattca atccttatca
     1501 tttagagtat ctcggaaaag aatcagatgt catcaacaaa ctgaagaagt ttgatttttt
     1561 tgcctgtgtg ggccataatg gcatccggga aaaaattcat actaacctga gtcagtacct
     1621 gggtaatcca atcaatgcca tacacccaag tgccgttata tcttcttcag tgaaaatggg
     1681 agatggtatc atgatcgctg ccaatgccac attgaacccg ttgatggaga tcggccgtgg
     1741 tgtgatttgt aatacctcta ccagtattga ccatgagtgt atcattggtg atttcaccca
     1801 tatcgcaccg ggagctgtac tctgcggaaa tgtaaaagtg ggccggaata catttattgg
     1861 cgctaactca gttattcgcc agggcatcat tatcggtgat aatgttacca ttggcgcggg
     1921 aacagtagtg gtaaaagata ttcctgatgg ggcaacagtg gttggtaacc cggcaaagaa
     1981 cctggtcaaa agacctgcac gcaaactggt ggcagcataa aatatgttct tccaaaaaat
     2041 aattatttaa agcccggtgt tctcgccggg cttttttatt gtaaaccagc cgggtagcag
     2101 tactgcaaaa ttgatcagtg cagtcagtta tatgattaat tctcaatacc tttgccactt
     2161 caaaaaagca gcataaccaa ccttattgtg aaaccagtaa acccgaagga cttcagcatt
     2221 ttgttccttt gcaaaaagga taatgagtat gcccaacggg cggctgcttt tgtaagggag
     2281 catttcacgg atcatttaat atttgaaggg gggcgcaagg ataaacttcc ccccgaagta
     2341 ttgaactggc ggggagattt attgatctct tttatctcct catggattta tccgcagcct
     2401 ctccttgaaa atgcattgat tgcagccatt aattttcatc ccggcagccc ggaatacccg
     2461 ggaaccggct gtaccaattt tgcaatttac aatagcgaaa aagaatacgg aattacctgt
     2521 caccatatga atgctacagt tgattcaggt agtattatac aggttaaacg gttccctata
     2581 aaagaagatg acactgttta tagtgtaacg cagcaatgtt ataaattgat cgaagaaagt
     2641 ttttatgaag taatggattg catactcaat aataaatctt tgccggttac taacgaacag
     2701 tggaaaagaa aaccatatac aagaaaacaa ttggatgagc tttgtaccat aacaccggaa
     2761 atgccggaag aggaaattga aagacgtatt aaagcgacaa cctataagac accctgggcc
     2821 tttaccaaaa taggcaatca tatttttaaa ttacaagcgg agaataagtg atgactacag
     2881 ctacaataaa tagaatatct aaaagccctg ttacgggaaa ggagtacgaa attccatctg
     2941 tattggatga taaggctggt attgatgcat ttatcgcatt gcaccccggt aagaaagtag
     3001 tggtggtgca gggattggga tttgttggtt ctgtaatggg cctggtggta gccaatgcac
     3061 tgacagagga atatgctgtc attggtattg atctgccaac accggcttct tactggaaga
     3121 tccgttcgat caatgaaggg gtattcccca tcattgccag cgatcctaaa attgaccttt
     3181 attatcagca agcattaacc aaaaaaaatt actacgctac ttatgatagc catgcttaca
     3241 gcaaagctga tgtggtagtg gtggatatta acctggatgt ccagaagaaa tctgcaaaaa
     3301 ataatgacct ggaaggatat gacgtagact taactccttt taaaaaagca attgaagcca
     3361 ttggcagtaa ttgtaaagaa gaagtactgg tactggtgga aactaccgtt cctcctggta
     3421 ctgcgcaaaa aattgtaaga ccattgctgg aagagtgttt aacaaaacgg ggcttgacaa
     3481 ctgataaatt aaaagtcggg cactcctatg agagagtgat gccgggtcct aaatatattg
     3541 attcgataca aaacttctac cgtgtctttg caggtacaga tgaaaaaagt gcagaggcgg
     3601 ttgaacgttt tttacggact gttattcgta cggatgagta tccgttgaca aggttaggga
     3661 ataccaatgc aactgagatg gcgaaagtgc ttgaaaattc tttccgtgcc atgaatatcg
     3721 cttttatggt agagtggagc cgttttgcag aagaggccgg agttgatatt tatgaagtgg
     3781 taaatgctat ccgtatgcgg ccaacgcata aaaacatcat gttgcctggt ttgggagtag
     3841 gaggatactg cctcaccaaa gatccgttac tggccagttg ggcaagaatg aatttatttg
     3901 gcagtgaaga aaaattggga caaagtgaaa aaggggttca catcaatgat aagatgcctt
     3961 tatatgcgtt tgaatatttg caatcgcagt atactggttc gctggccggt aagaaagtgt
     4021 tattgctcgg tgtaagttat ttgaatgatg taggtgatac ccgttacaca ccggtagaag
     4081 gtttttatga tcagttgata aatgccggct gtgaaattac cctgcatgat ccgcatgtaa
     4141 aatactggga agagaaagat atttggataa atcagaattt ggaagagctg ttggaagatg
     4201 agtgtgatgt ggtggtggtt acaacggggc ataaagatta cagaagtaat gaaagtctta
     4261 ttaatacttt attaagtaag cctgcagctt ttatgtatga tacaattggt gtgtggacga
     4321 aagacgaaat aaaggcactg ggcgaaaaac atatggttaa agtgttgggt aggggtgatt
     4381 tgtaggatgt gaacacggat taagcggata ggcacagatt tttatattga aagagttgtg
     4441 aatcttgtga ctgctccgca ggggctgaat ttgaataact ccgggtgaaa cccggagaaa
     4501 ggaaagcgac aaatgtatta gaatccctta ggggttcaat gtgcacgaaa ggaaagtata
     4561 gatgaagtgt aaggtagtag cacatgctga atagaattac tgaacgatga agtgtgcgac
     4621 gcaacaaaag ctgatacctg ccggaaggca ggcaaggcag cacaggcgat gacaaaaaaa
     4681 tattacaaca acagatatga gtaaaaacgt tttattactg ggcggtgccg gttttattgg
     4741 tttcaatatt gccaggtacc tggcagaaaa ccgggactac aaactaacta ttgctgacaa
     4801 ttttgcaaga ggaaagcaag atgattattt tacggaactg gtaaataagc ataacataag
     4861 agttgtagcc ggcgatttta ccgacccagt tactttcaat cagctggatg aagcctatga
     4921 ccaggtttat atgctggctt ctgttgtagg ggtggataat gcaaattcta ttccgcatga
     4981 gatcatccgt atcaacacag ctttgatctt taatacattg gaatgggtac gtcgttctaa
     5041 agttggtaaa gtattgttca catccaccag tgaatgttac gcaggaacga ttgaagcatt
     5101 tggatatgtt acacctacac cggaagaagt acccttatgt atacaggata tcgggcatcc
     5161 aaggtttacc tatgcggtta ccaaaatgct gggtgaatca ggttttctca actacgcaag
     5221 gatactgggt tttgaaacaa caatagttcg ctatcataat gtgtacggcc cgagaatggg
     5281 gttcaaacat gttattccac acctggtggt tcgttataga aaaggtgagg agccattcaa
     5341 agtgtatggt catgaccaga caagagcttt ttgctatatc accgatgcgg ttgaaggcac
     5401 tgtgcttgcc atggagcagc ccaatacaaa tggggagatc tatcatatcg gtacgcagga
     5461 tgaaattaca attggcgaat tgattcatta tgccggggaa ttgatgaatt ttaagggtac
     5521 ttatgagaat gcaccaacct atcctggttc ggtatccagg cgttgtcctg atattacaaa
     5581 ggcgaggact caattgggtt ttgaacctaa agtgatgtgg aaagagggat taaaaaatac
     5641 agtagagtgg tataatcaat atcttgaatc cggaaaatta gtttatgagt aataacaggt
     5701 ttattccgct cagtgttccc aatattgcgg gaaatgaatg gaagtatgtg aaagattgtt
     5761 tggataccgg ctggatttca tctgttggtt cttatgttac acagtttgaa gaaatggttg
     5821 ctggatttgc cggcgctaaa tatggtgtgg cggctgtcaa tggaacggca gcattgcata
     5881 tttctttatt gcttagtggt gtaaaaagag atgactatgt tattctgcct aatctcacat
     5941 ttgtagcttc agccaattcc ataaaatatc ttggcgcaga acctttgctg attgatgcag
     6001 acccggactt atggcaaatg gatcttgatt tgctggaaga atttctggag aatgaaacgg
     6061 atgaaaagaa tggtgagctt atttatataa aagatggccg gaggataggc gctattatgc
     6121 cggtgcatat tctcggtaat atgtgtgata tggacaggtt tcttgccata gtcaaaaaat
     6181 atccgttgcc aattgtggaa gattcaacgg aagcattggg taccacttat aaaggaaaga
     6241 gtgccggttc attcagcccc cttgcctgtt ttagttttaa cggtaataaa attatatcta
     6301 ccggcggcgg gggtgtcata gttactgatg atgaaacatt ggcaaaacat gccaagcacc
     6361 tgaccactac tgcgaaggca agtcctgatg aatattatca tgatgaagtg gggtataatt
     6421 accggttggt aaatgtattg gcagccatag gtgtagggca aatggagttg cttccatcat
     6481 tcataaaacg gaaaagggag tgtgtggcct tttataaaaa ggaattggct ggtataggtg
     6541 atattcgttt ccagaaagaa ttgcctgatg tggagaccaa tgggtggtta tttaccatac
     6601 agacagataa gcagatacaa ttgctggatc acctgaatgc gaataagata ctaagccgcc
     6661 gtttctggat gccgatgaat aaactgccga tgtacaggga ttgtgtatat gtacagaaaa
     6721 aagacacctc agattatatt tataatacct gcctgagtat acctagttcc accagtatta
     6781 cggatgatga actggccatt gtcatccggg aaattaaagc agccatcgga taaattttat
     6841 gtatcaaata atcaaacgtt ttttcgacat cgtcttttca ctggtagcta ttatcattct
     6901 gctccctgta tttataccgg ttatcctact gttgttgctt accggcgaac atgaagtatt
     6961 ttacaagcag gatagggtgg gatataaaaa caggattttc aaaatcttaa agtttgccac
     7021 catgctgaag aatagtccca acatgggcga tggtgatatt accaaacggg gcgacccaag
     7081 aattactaag gtgggaaaat tgttgcgtca aagtaaactg aacgagttgc cgcaggtatt
     7141 taatatcttt ttgggcgata tgtcttttgt gggaccaagg ccattgatga aagttggttt
     7201 tgaccgctat tctgatgaaa tgaagtcaaa agtgtataat gcacaacccg ggctaaccgg
     7261 tattggctct attgtgttca gggacgaaga actgataatc acccaaagca atttacctcc
     7321 tcatgattgt taccgggata taattcttcc ctataaaggg gcgctggaag tttggtacaa
     7381 gcagcaccag aacttcttca ccgactttat gattttgttt ctgacagcct ggtatattgt
     7441 tttcccaaaa agcaacatgg tgtataaagt ttttccctct ttgcctaaaa gaagtttctg
     7501 aacaggttta atttcattgt aatttcggta acaaagcctg cgtaataatt atgtttaaac
     7561 tgttttcaaa gtcaaaaaaa aatgagtcgg cagaaatagc tgaattcggt ctcctgaaaa
     7621 cagatatgca ctcacacctg atacccggaa ttgatgatgg ggctcccgat attgagacct
     7681 cattgaagct tatcggagga ttacattcac tgggctataa aaaaatcatt accactcccc
     7741 atattatgtg ggatatgtat aagaatacaa gagagatcat actggaaaga ctggagtggt
     7801 taaggacaac ggtgaaagaa gcagaacttg atatagagat tgatgctgct gcagagtatt
     7861 tcctggatga tcatgtagca ggactgctgg caaataatga gcctttactt acggtgagcg
     7921 gtaatatggt tttagttgag ttttcgatgg cttatccttc tcatagtctg aagggaatat
     7981 tatttgatat gcagatgcag ggctatcagc ccatcatcgc acaccccgag cggtatatat
     8041 acctcgatca gaacaagggc ttttatgaag agctaaaaga tatcgggtgt ctgttccagt
     8101 taaacatact ttcattgacg ggatattatg gtaaatcggt acaggaactg gcccagtacc
     8161 tgatgaaaaa agaatattat gacctggtcg gaacggattt acatcatttc ggtcacctgg
     8221 aagctctcag tacaagacct gtcttggctg cccagctcaa gaaattatta gacagcggaa
     8281 agattaagaa ctttgagctt tgatgactgt attttccttt tttgaatact tttttagaac
     8341 aaacctgaac ttttttcagt gagggttgtc tctattacgt catgaatgcc tttaccatta
     8401 aagatttaga gaatttatcc gggatcaaag ctcatacaat acgtatttgg gagcagcggt
     8461 ataattttct aaaacctcag cgaaccgata ctaatatccg ttactacagt agtgatgaat
     8521 taaagacagt attgaatata gcattgttaa ataagtatgg gtttaagatt tcgcatattg
     8581 acaggatgca gcctgaagaa attaaagcaa aaattttatc attaggcgat gcaaaggcaa
     8641 tacaggaaag gatagtgaat gagttggtgc aggaaatggt ggatctgaat attgagaaac
     8701 tggaaaaaat tctggccaat tatatttcct taaggggtat tgagaaaact gtaatacata
     8761 ttattttccc cttcctggag aaaataggtc ttctctgggt aacgggtcat atcaaccctg
     8821 ctcaggagca cctagttaca aatgttatac gacaaaaatt gattgttgca atagaaacag
     8881 ctattaccca cctgaaagtg gacaaaactg tactcctttt tctaccggag ggggaacatc
     8941 acgaattagg cctgcttttc atgtactata tgcttaaaaa caggggtatt caaacaattt
     9001 atcttggggc caatgttccg gttaaagatg ttgcctatgt ggcgagactt aaaaaaccag
     9061 atatcgtttt tacacatctt accgccactt cttcaagctt taactttgag aaatttttaa
     9121 atagtatcaa ttctcaattt aatgatatac aaacagttat atctggccaa ttaactcact
     9181 cctatactaa gaacattcca aacgggatta aattcaaaaa atccctccag gaagtaatgg
     9241 agtacctcac ttcgctttaa taaaaattct actggttaag taattgatcc ctatttgttt
     9301 aatattttct tcaaaatatt aaacattttt tttggctgtc taacataatt atgtttaact
     9361 tcacacttca attaattaaa caataattta tgtctagcgt agaattcaat caaatgcttc
     9421 tcagcaatgc agatttcctg aaaccgtttg ctgtaaacct aaccagggat actgaagcgg
     9481 cgaatgattt gtaccaggaa accttgtaca aagccctggc aaaccaggac aagtacaatt
     9541 caggtactaa tatcaaggca tggttgttta ctatcatgag aaatattttt attaataatt
     9601 atcgtagaaa agctaaacaa aaaaccattt ttgacagcac tcctaatgat tatcttatta
     9661 atcagaaaca ggccacagtt agtaatgctg ccgaaagtga tatgcggatc aaggaaatta
     9721 aaaatgctat tcagcaatta cccgaaatat ttaagactcc ctttttgttg tactttgatg
     9781 gttataaata caatgaaatt gcggatgtgc ttaatgaacc cctgggaacc attaaaagca
     9841 ggatccactt tgcaagaaaa ttattaaaag aacaaatcag tcgcttctaa aaaaaagctg
     9901 gtgtcagata aatcagttat tgttataggt agtggttttg ccggcttgtc cgcagcatct
     9961 ttcatggcct cagctggctg gaaagtaact gttattgaaa agaatagtac acccgggggt
    10021 cgggcaagac aattaaaaga agatggcttt acgttcgaca tgggtcccag tttctattgg
    10081 atgcctgatg tatttgaaag atactttgcc caatttggaa aaaaggtctc tgattattat
    10141 tctttgcacc gtttagatcc ttcttatcgg atttactgga atgatggcta taccgacatg
    10201 ccggccgatc tttttgaact caaaaaagtt tttgaatcaa tagaacccgg tagcgctgaa
    10261 aaattagaaa aatatctcaa aggagctgcc tataagtatg aggtcggtgt aaataagctg
    10321 gtctataaac ccagccgttc tttaactgag ttcatggact ggcaaacgat cagtggtgta
    10381 ttcaaactgg aagtgtttac ttctatcaag aaacatattg agaaatattt taaacatccg
    10441 aagctccggc agatgatgga attccctgtt ttatttctgg gtgctttgcc ggaagataca
    10501 ccggccctgt acagcctgat gaattatgca gatatcaaag gaggcacttg gtatccgcaa
    10561 ggtggtatgt atgccatcgt ggagggtatg tatcaactgg ctatggagtt gggagtgact
    10621 tttcattttg aagaaagtgt gcaagaaata actattgatg atagtattgc gaaaaaagtg
    10681 gttactgata aaaattcgta tgaagcagat gctgttatca gcggtgctga ttatcatttt
    10741 actgaaaaga acctgctcgc agcgcaatac cgcagttact ccgatcaata ttgggacaaa
    10801 aaggtgatgg cgccttcctg tctgttgtat tatgtagggt tgaataaaaa gctcaaacag
    10861 ccaattcatc attccttatt ttttgatgtg ccttttgagc agcatgggga agagatttat
    10921 aaaaatccta aatggccggc cgaacccttg ttttatgtta gtgtttcctc gcttacagat
    10981 gattcagtag ctccagaagg acatgaaaat attgtttttt taatacccgt tgcttccggt
    11041 ttggaaaatg atactgagga gcttagagaa aaatatttcc aggtaatagc caggagaatg
    11101 gaaaaacata ttggtgagtc catacttgat tcagtcgttt ataagaaaac ctattcagtg
    11161 agtgattttg taacagatta caattcattt cgcggtaatg cgtatggatt ggcgaatacg
    11221 ctgctgcaaa cagctatttt ccgcccgtca tgccaaagta aaaaagtgaa aaatttattt
    11281 tataccgggc aattaactgt tccgggacca ggtgttcctc cttcgttgat aagtggtgag
    11341 gtagtggcta aacaagttat caaagctttc aaataattac ttaaattgaa taatggttca
    11401 actgtttcat accacaagtg aaaattgcag ccgcattata acggagaatt acagtacctc
    11461 attttcttcg gctatacgta tgctgcataa ggatttgcgt acacctatct ttaacattta
    11521 tggatttgta cgctttgcag atgaaattgt agatacattt catgactatg acaaagcagc
    11581 tctcctggag cagtttaaaa aggaaactta tgatgctatc caaaggggaa tcagtttgaa
    11641 tcctgcactc aatagttttc agcgtacggt aaatgaatat aatattgacc tgaaactggt
    11701 cgatgcattt tttcatagta tggaaatgga cctgagtaag caggtatatg attgcgaagg
    11761 atacaaagaa tacatatatg gttctgcaga ggtggttggc ctgatgtgtt tgtatgtttt
    11821 ttgtgaagga gagaagagcc tgtataataa attagaattt tctgcaaaag cactgggagc
    11881 tgcatttcaa aaagttaatt ttttaaggga tataaaagcg gattataacg ggttgtcaag
    11941 gatttatttt cccggttgcg actttgctaa tttttcagaa agtgaaaaaa aagctataga
    12001 acaggatatt cagaatgatt ttgaagctgc ttatgagggg ataaaaatgt taccgctgaa
    12061 agcaaggttc ggggtctatg ttgcctataa atattatttg tctttattta agaaaataaa
    12121 aaaattggaa cctgaacggg ttttagatac cagaatacga attcctaatt acctgaaagc
    12181 catgatagtt ttaagggcag gggtaaaaaa ccaattgagg ctgatttgat atggatctta
    12241 cacaggttat attggtagac cagcaggata atcccaccgg tagttgtgaa aaattagaag
    12301 cccaccagaa agggttactc cacagggcat tcagcgtttt tatattcaat aagaaaggag
    12361 agatgttatt acagcaacgg gcagttaata agtaccatag cggtggattg tggaccaatg
    12421 cctgttgtag tcatcctgct gaaggagagg atactattgc ggcggcacat agaagattag
    12481 tggaagaaat ggggtttgac acaccagtgg aaaaagtatt tgattttgtt tacaaagcag
    12541 attttgataa cgggctcacg gagtatgagt ttgaccatgt ttttgtgggc gaatacagcg
    12601 gcgcaattaa ttttaataag gaagaagtga tggattattg ttataaaacc gtccaggcaa
    12661 taggtctttc actgcaatcg catcctcaaa aatatacggc ctggtttcac ctggcctttc
    12721 ccaaaattga aaattggtgg aaaaagcatt ttaataacca aacgacgtaa aaaaattaat
    12781 tttgtaatca catggatata ctattataca ttctcgtatt actcggaacc tttattgtaa
    12841 tggaaggagt tacctggtgc acacataagt ttgtaatgca tggattctta tggtatttgc
    12901 ataaagatca tcacaaagta caacccggga tctttgagaa aaatgatgct ttttctataa
    12961 tttttgcagt tcccagtata ctcctgattt atttcggtac ttataatgat gtatggtggc
    13021 agcaggctat aggttttggt attatggctt atgggtttgc ttatttcatg gtacatgatg
    13081 tgatcataca tcagcgcttt aaatggttca ccaaaagtaa aagcacctat gtcagggcca
    13141 tcagatgggc gcataaaatg catcataagc atattgataa acatgatggt gaaagtttcg
    13201 gattcctctt tgtaaataaa aaatactggg ataaagtccg tcgtgacgat aaattaatgg
    13261 ccggtagtaa gaaagtggaa tatgccccgg aagcagagta attacatgag caactcctat
    13321 ctttattttt caattagttg atctgcttaa atctaattca tacgacttta ttttctgtgg
    13381 tgctgggtgt gccagtctta gtttattaat gcggatgatc cgctctggga aattcactga
    13441 caagaaaatt ctcttgctgg ataaagagcc caaaacaatg aatgaccgca cctggtgctt
    13501 ttgggaaagg gaaaatggtt ttttcgatga aattgtttac agaaaatggg acaccatttc
    13561 attttttggt gataattttt catccccgat ggatattgct ccttaccagt ataagatgat
    13621 aagaggaatc gatttctacg ggtattgttt tgcagcaata aaaaagcagg agaatattga
    13681 aatcaggtat gcggccattg gagaatggtt tatgcaggag gaagaagcta ctttcattct
    13741 tgatggtctg ccatattccg taggcagacc caccgttttt aattctattt acgagcctgc
    13801 aaagaaaaat gaaaatataa tcaggctttt acagcatttc aaagggtggg tgatagagac
    13861 tgacaggccc ttctttcacc cggaacaagc gacaatgatg gatttcaggg tgtcccagca
    13921 acatggaact acttttgcct atgtgcttcc cttttctgct actactgcat tggtcgaata
    13981 cactttgttt acaaaaaatt tgctggaacc tcatcaatac gatgatggtt tgaaagaata
    14041 catacactcc tttctggaga tcagcaatta cacaattaag gaagaagagt ttggagtcat
    14101 accaatgact aacgaaaaac acagttttga cggacatggt tggcaaatag gtaccgccgg
    14161 cggccaaaca aaagcttcca gtggttacac attccagttt attcagaagc aatcacagca
    14221 gatagttgaa tgccttctta gcggccggtc attggctctt attccggcaa ctccaaagag
    14281 attcaggttt tacgacaata ccctccttga tattctgtat aatgatacac tccccggtaa
    14341 acaaatattt acacagttgt tcaaaaaaaa taaacctcag caggtgctga ggttcctgga
    14401 taatgaaagt tctttaaaag aagaattgaa aattattgct actttaccca cctggccgtt
    14461 ctttaaagca gccttaaagc agttataatc aattatcagg atccatctgc ataaattctc
    14521 tttttactgc tttcagcata ctgaatagtt gtgctacctg tatcagcagt aagaccgcta
    14581 atgccagcat tacataccag ggaagtgtaa tcgtttttac acccggtatc ttttggaatt
    14641 tttcaatatg ccacccggaa agagctgagc caaaagccat acccactaat agaaaagaaa
    14701 ttaacgaata gactactttc atgaacatga ttctcattgt cgagtaacct actttaaaat
    14761 tttcgagtat tgcggcaggt ataatcagca ctaaagcaat ccagctccag ttcttactga
    14821 accattcatt gtccagtgtc agattgagtc caagtaccaa tacgatagaa agtagtcccc
    14881 aggggaatag cagaaaagta gaaggtttat gtgatgtagt tgccatgccg gtagattttt
    14941 gttaggagag taagttaaaa ataatcttcg ataaaaggaa aagcatccta tgcaggatgc
    15001 tttgtagtag cctcgacagg aatcgaacct gtatctggag cttcggaaac tcttatacta
    15061 tccattgtac tacgaggcca gtaactg
//
LOCUS       DAORTI010000086        14858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3836_length_14858_cov_7.223130, whole genome shotgun sequence.
ACCESSION   DAORTI010000086 DAORTI010000000
VERSION     DAORTI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14858
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_14858_cov_7.223130"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="PLO70_11205"
     CDS             <1..2085
                     /locus_tag="PLO70_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75078.1"
                     /translation="TITGSNLTTADVTVAALAGYTYSTTAGGTYTTTLNITQPGATFS
                     QQVFVKFDPVAVQSYNGNIAVAGGGVTTAINAAASGAGVNTIPSLTTGSATAITQVSA
                     TLAGTITATGCTPVTVYGIEYSTTSGFPNGSGIQVASTNISGGNYTSALAGLTSGTTF
                     YYKAYATNAGGTGYGTEQSFTTATPNPTINTTALTAFGNVCTNVTAGPNSFTISGANL
                     TTADVTVGPLAGFTFSTTAGGTYTASLSLTQPGGTYSQDVFVKFTPVAVQSYSGDIPV
                     AGGGVAATVNVSASGAGVNTAPSVSTGGASAITTTTASLAGTISAIGCTSVTVYGIEY
                     SATNGFPNGSGIQVVSTNISGGNFTSGLSGLIPSTTYYYKAFATNAGGTTYGVQQSFT
                     TAAPPPPVMTATALTPFGNACITTTAGPNSFTINGANLTAVPLSVGPLAGYTFSETSG
                     GTYVTTLAIAQTGGTQSKTVFVKFTPTAVQSYNGNIPVSGGGAPVITVAASGSGVNTL
                     ATVITGAATGLTTQSAVLAGSVSDNGCSNVTTYGIEYSGVNGFANGTGIQVASSNLTG
                     GNFTISLPGLVQGATYYYKAYAVNNGGISYGLQQSFTVLSINPGFSLYPSPAVRGTDV
                     RLTMTDLTPGYYGLLLFSSNGQLVFQRNMNIQAGFINQAFTIPATLSPGVYTAYLVNH
                     LITIGTRTILVQ"
     gene            complement(2255..2836)
                     /locus_tag="PLO70_11210"
     CDS             complement(2255..2836)
                     /locus_tag="PLO70_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ75079.1"
                     /translation="MKALLKKTDHELIHLFQDGNLEALEALVLRHKDKLYTSILFLVK
                     DKYLAEDIFQDAFIRIIDTMRGGRYTEEGKFLPWAMRIAHNLCVDHFRKVKRTPTIRN
                     GEDKDIFEVLNFSEDSAETVMMKRQSHDRVRDMLQSLPEDQREVIILRHYADMSFKEI
                     AAATNCSINTALGRMRYGLINLRKLMTQKQIAL"
     gene            3122..5956
                     /gene="uvrA"
                     /locus_tag="PLO70_11215"
     CDS             3122..5956
                     /gene="uvrA"
                     /locus_tag="PLO70_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQZ75080.1"
                     /translation="MEKSIIGKNPAKPKTVISAENIQIRGARVHNLKNVTVEIPRNKL
                     IVVTGVSGSGKSSLTIDTLYAEGQRRYAESLSAYARQFLNRMNKPDVDYIKGLCPAIA
                     IEQKVITRTPRSTVGSMTEIYDYLRLLFARIGKTISPVSGKEVKKDDVTDVLDAIIKL
                     KEGDKVYILATFKQHKNRDIKEELNILVQKGFSRVYSRDPGAGNEGQIFRIEELLELS
                     DKELKSKKITHILVDRIVVKEFDEDDKHRMADSIGTAFYEGEGDVYIEVNGKKVVNFN
                     NRFELDGIQFEEPVPNLFSFNNPFGACPTCEGFSQVLGIDPDLVIPDRRLSVYDGAVA
                     PWKGEKMDWWRQQFIKVARKVNFPVHKPIADLTDKQYKTLWDGVDGIGISDFFKDVES
                     QLYKVQYRVMLSRYRGRTECPDCNGFRLRKEALYVKVNGRHIGELSTMPVKDLKVWFD
                     ILANKLSSHDKEVGKRILIELHGRLDTLLKVGLGYLTLSRLANSLSGGESQRIQLTRS
                     LGSNLTNSLYILDEPSIGLHSRDTANLIKVLKELRDLGNTVVVVEHDEMMMREADHII
                     DMGPYASHLGGEVVAEGDYDDIISNPMSLTGKYLKGELTIDPPKTVRKWNRSVKVEGA
                     RHNNLKNITVEFPLNVLCVVSGVSGSGKTTLVKQILYPALRKIKGEFGDKVGFHKAIT
                     GDIDGIGQLEMVDQNPIGKSSRSNPVTYIKAYDEIRDLFASQPLSKMRGFMPKHFSFN
                     VDGGRCDTCKGEGEQVVEMQFLADVHLTCESCGGKRFKEEVLEVKYRDKNIFDVLEMS
                     VDEAINFFYEEKNLAKAIQPLSDVGLGYVKLGQSSDTLSGGEAQRVKLASFLGKGRAN
                     NKILFIFDEPTTGLHFHDINKLLASFNALIEQGHSIIVIEHNTDVIRCADWVIDLGPE
                     AGDGGGELVFAGKPADLKKSKQSHTAEYLL"
     gene            6290..8170
                     /locus_tag="PLO70_11220"
     CDS             6290..8170
                     /locus_tag="PLO70_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75081.1"
                     /translation="MKKIYSLMMFTLIVVLFTTNNVFGQNEGDYRSDQDGNWNSVVTW
                     DVWDGDSWENATTIQGYPGQYSSPASVTIRNGDDVTFNVSPAFSIGALVIEAGGSTSI
                     LDFNSGTTLNVSGGITIGGGTGNGDDKRIDVGDGILTCASITMLTTGNDGADSEVRID
                     DGTVTVSGDITMNDVATRNFIEFTNPGTLNVGGSISAGGDIVPNNATEGLVNYNGTSG
                     TQTVKSDTYADITFSGGAVKSIATGGIIITDVATFTNGIVNSSSTNSITFNDNSTTTG
                     ANDASFVNGPIIKVGNDAFDFPVGTTIGGNRYHPISIGAPSASTTFEAEYIRASSVLL
                     EPDLITGVYAVSACEYWRLDRTGAAATADVTLYWNEYSPCGGAYITTLTGLIVAWRDL
                     ADSRWENSLGGPQTITSVSTYTTGTLLRTSVDANGFGYFTFGSANINGSPLPVKLSNI
                     RAFEKQQGIQIDWTAYQEDNLNRYQVERSADGQSFMPIGDVAARNLVIESNYSFFDAN
                     PLPGVNFYRLKNIDMDGKSGYSNVVKVNLDKNIKGFTLYPNPVRNGLVSFQSSDLSKG
                     NYSVNIFNANGQQVYSQRFSHSGGSVNQTLQLPAGVRSGMYTMQVDNGTMKLMSKTFM
                     VQ"
     assembly_gap    8525..8624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8625..10982
                     /locus_tag="PLO70_11225"
     CDS             <8625..10982
                     /locus_tag="PLO70_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75082.1"
                     /translation="SFSINRSYNTNAANVITTVSGTLRLVSNNGTIVNATPAKLFFTA
                     GSTYEHSRNGGTIPTAAWNAASNCFVTGITGTAPTGLNQSFGNFSWDCTGQSTGFLLN
                     GVLTTVNGNLSVGNTNLFGVFLATASNSSYTLDIGGDFIINDNAWLSITNGDNITATV
                     NVAGDFNMTGISSSSTFFDYHAATGGAVTLNKIIMNIAGDFIQSGGLFDFAYGDSDAS
                     NFTELRLSGNLTISNSGLMQTGSADNSVSNGTIIFNKPGIQTFFAATPANIAYVNYVV
                     NNGSTLEMLSDISLSSIATAVWAGQFTVNAGAVLNIDSNRIVSSSGATAGVNNSFILN
                     DGAVIISANPDGLQHNETIGSVSTAIATRTFRSNADYEFKGAVTGTFITTPIENTARD
                     IIINNTSGNVTLSQPMAVTRSFILASGNLITTITNLLTINDDATSSAGSYSPVRYVDG
                     PVRKIGDDTFTFPVGKSGVYAPISISAPGTVNDEFLAEYMRTTPPNRFSITAPGLNHI
                     SFCEYWNLEEIGPGSPTVNVSLSWSGLSPCNTAAYINDLATLTVAQYNGTSWDSHGNS
                     GGTTGSGSLGTVTRNNVSVFTPFSIGSTSAGTNPLTVKFSLVKAYPAGNINKIEWTNS
                     SEEGVAQYEVERSTNGISFVMIKNINARSNAGTREDYVVSDENPYPDISFYRIKAVEI
                     NGVISYSPVIKIIRNTTADMKLQVYPNPVVNRQFVIQLRNQQRGNYLLKLFNNSGQEV
                     YQTNWQFAGGSASRTIELPSGIIPGMYHVQLSANESMLQSKIIIR"
     gene            complement(11058..11480)
                     /locus_tag="PLO70_11230"
     CDS             complement(11058..11480)
                     /locus_tag="PLO70_11230"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HQZ75083.1"
                     /translation="MIQRQQTLWLLLSTAAAIFSFMFPFVVGDEMQQNSPSPVRTVID
                     AGSNFFLLLLTGGSLILSTVIIFLFKNRKQQIWLCLLGILVAGLLIFLYVLEMNKLIK
                     PIPALTSVLPLFILIGYFMAFRNIRKDEKLVKSLDKLR"
     gene            complement(11520..12797)
                     /gene="glyA"
                     /locus_tag="PLO70_11235"
     CDS             complement(11520..12797)
                     /gene="glyA"
                     /locus_tag="PLO70_11235"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQZ75084.1"
                     /translation="MQKDTLVFELIRKELDRQRNGIELIASENFTSLQVMQAMGTVPT
                     NKYAEGYPGKRYYGGCEVVDEIENLAIERLKKVFNASWANVQPHSGASANAAVFLAVM
                     KPGEKFIGLDLSMGGHLTHGSPANFSGKTYHAIHYGVTKDGIVDYEQLEAKARAEKPK
                     MIICGASAYSRDWDYKRIRAVADEVGAIVFADIAHPAGLIASGLLNDPFDHCHIVSST
                     THKTLRGPRGGIIMLRHDFENPWGIKDQKGNLRTMSQLLDLAVFPGSQGGPLEHVIAA
                     KAVAFGEILTEDFKAYGQQVIANAQAMAKAFVNRGYQLISNGTDNHLMLIDLRNKNLT
                     GKKAQETLDKAHITLNKNAVPFDDKSPFVTSGIRVGVPAITTRGLQEGHMETVVSLID
                     KVLMNADNEEVIAAVKSEVKAFMQQFPLYPELG"
     gene            12935..13924
                     /locus_tag="PLO70_11240"
     CDS             12935..13924
                     /locus_tag="PLO70_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQZ75085.1"
                     /translation="MKAIIPVAGAGTKLRPHSYTQPKALIPLAGKTILSIIIDQLKEA
                     GIDEFIFIVGYLGEKIEDYVKLKYPDLVAHYVHQTDRQGVGHAVRLTRNLVNQDEVFV
                     VLGDTICEYDVKEVVNSPDSMIGVRKVDDPRDFGVAEINEDCYIEHVVEKPQIPKSNM
                     AMVGIYKIKESEQLFQCLENNIRQGLRSHGEYSLTDALDCMIKAGAKFKSFKVENWFD
                     CGRKETLLESNALLLKKFAPQTDAGRFENTVIVPPVSIGEGCTIKNSIIGPNVTIGEN
                     TTVDYSIVKDSIIGSFSNLFDIVLDDSVIGSDTNLHGESRSLNIGDNTSIDLG"
     gene            14012..>14858
                     /locus_tag="PLO70_11245"
     CDS             14012..>14858
                     /locus_tag="PLO70_11245"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQZ75086.1"
                     /translation="MTRFSNRITQLFNIDFPIIQAGMIWASGWRLASAVSNAGGLGII
                     GAGSMYPEVLKEHIQKCKTATNRPFGVNLPLLYPDIDKHIQIIIEEEVKIVFTSAGNP
                     KTWTSLLKEKGITVVHVVSSSKFAKKAEDAGCDAVVAEGFEAGGHNGKEETTTLVLIP
                     AVVNAVKIPVIAAGGIATGRQMLAAMVLGAEGVQMGSRFVACEEASSHINFKNVVINS
                     QEGDTQLALKQLTPVRLIKNDFFRQVQQAEQRGATVEELSTILGRARAKKGMFEGDLQ
                     EGELEI"
BASE COUNT         4340 a         2811 c         3338 g         4269 t
ORIGIN      
        1 actattaccg gttcaaatct tactacagcg gatgttacag tagctgcttt agccggctat
       61 acttattcaa caacagcggg aggtacttat acgacaacat taaacattac acaaccaggg
      121 gcaacttttt ctcaacaggt atttgtgaaa tttgaccctg ttgctgtaca gtcttacaat
      181 ggaaatattg ctgtagccgg aggtggtgta acaacagcca tcaatgcggc agcttcaggt
      241 gccggcgtta atactattcc gtcactgaca accggctctg ccacagctat aacacaggtt
      301 tcagcaacac tggccggtac aatcacggct actggctgta ctcctgttac tgtttatgga
      361 atagaataca gtacaacaag cggatttccc aatggatcgg gaatacaggt tgcttctacc
      421 aatatttccg gtggcaacta tacttcagcc cttgcgggtc tgacctcagg aacgactttt
      481 tattacaaag cttatgctac taatgctggc ggcaccggtt atggaactga gcagtctttc
      541 acaactgcca cacctaatcc aacaataaat acaactgcat tgacagcgtt tggtaatgtt
      601 tgtaccaatg taacagccgg tccaaattcg tttaccatat cgggtgctaa tcttacaaca
      661 gctgatgtaa ctgttggtcc gttggctgga tttactttct ccactacagc cggaggaact
      721 tatactgcat cgcttagcct tacccaaccc ggtggcacct actcacaaga tgtatttgtg
      781 aaatttacac ctgttgctgt tcaatcgtat agtggtgata tacctgtagc cggcggtggc
      841 gtagcagcaa ctgtgaatgt gagtgcatca ggtgccggtg ttaatactgc accctctgtt
      901 agtacaggtg gtgccagtgc tataacaacc actactgctt cactggctgg tactatctct
      961 gctattggat gtacttcagt gactgtgtat ggtattgaat atagtgccac taacggattc
     1021 cccaatggta gtggtataca agttgtgtct actaatattt cgggagggaa ctttacatct
     1081 ggtttaagtg gattaatacc gagtacaacg tattattata aagcgtttgc caccaacgca
     1141 ggtggaacta catatggagt gcagcaatct ttcactacag cggctccgcc gccaccggta
     1201 atgacagcaa cagccctaac accatttgga aatgcctgta taaccactac tgccggcccc
     1261 aattcattta caattaatgg tgctaacctg acagctgttc cattatcagt tggtccatta
     1321 gcaggatata cattttcgga aacttccggc ggcacttatg taactacatt ggctattgct
     1381 cagacagggg gtacacaaag caaaactgtc tttgtgaaat ttacgccaac agcagtacaa
     1441 tcttataatg gtaatatacc cgtaagtggg ggaggtgctc cggttattac tgtagctgct
     1501 tcgggcagtg gagttaatac actggccacc gtaattactg gtgctgcaac agggcttaca
     1561 acacaatctg ctgtactggc gggttctgtt tccgacaatg gttgttctaa tgtaactact
     1621 tatggtattg aatacagcgg tgtgaacgga tttgccaatg gaacaggtat ccaggtagct
     1681 tcttctaatc ttaccggagg aaatttcacc atatcattac ccgggcttgt tcagggtgct
     1741 acctactatt acaaggctta tgcagttaat aacggaggaa tatcttatgg tcttcaacag
     1801 tcatttaccg tattgtcaat taacccggga ttcagcttat atccgagccc ggctgtaagg
     1861 ggaactgatg taaggttaac aatgactgat ctaacaccgg gctattatgg cttgttatta
     1921 ttcagcagta acgggcaact ggtatttcag cggaatatga acattcaggc aggatttatc
     1981 aaccaggcat ttacaatccc ggcaactttg tcaccaggtg tttacaccgc ttaccttgta
     2041 aatcatctta tcactatcgg aaccagaact atcctggtac agtagtaagt atagtctgat
     2101 taaaaagtaa aaaagcctcc tgttacataa ttgtagcagg aggcttttca atttggtgtc
     2161 attctgtacc agccttccgg cttggcaagc aaacgtaaat tccgtttaat cagaatctgt
     2221 tttactccgg atggagatac agaatgacaa gagcttataa agcaatttgt ttttgtgtca
     2281 tcagcttacg caaattaatt aatccgtaac gcattcttcc cagggcagtg ttaatactgc
     2341 aattggtggc tgcggcgatt tctttaaaac tcatatctgc ataatgacgt agtatgataa
     2401 cttctctttg gtcttccggc aaactttgca gcatatcacg aaccctgtca tgactttgcc
     2461 ttttcatcat tactgtttca gcactgtctt cagaaaaatt cagtacttca aaaatatctt
     2521 tgtcttcacc attacgaata gtgggggtac gttttacttt tctaaaatgg tcaacacata
     2581 agttatgtgc tatacgcatg gcccagggaa gaaattttcc ttcttcggtg tagcggccac
     2641 cccgcatagt gtcaataatc cggataaagg catcctggaa aatgtcttcc gctaaatatt
     2701 tgtcttttac aaggaagagg attgacgtat agagtttatc cttatgccgg agcaccagtg
     2761 cttctaatgc ttcaagattt ccatcctgga acaggtggat gagttcatgg tcggtttttt
     2821 ttaataaagc tttcataact tctacaggtt taggttgcac tgtacagaca tttgcatgca
     2881 cagttattca aatggatatt ggttcaaaaa atcggttaaa aataaagagt agaagtttgt
     2941 gcgataggct gtgtgagttt tgggtttttc attttttgga taattggaaa ctaaaaatag
     3001 accatttcac gaaattgcca aacatttgta aaagcaatat gtagtatatg ctgctaaata
     3061 aatgttaatg atttaggctt aaacccgcat gggataaggc tttacatttg caagtataat
     3121 aatggaaaag tcaataatag ggaaaaatcc cgccaaaccg aagaccgtaa tttccgcaga
     3181 aaatatacaa ataaggggtg caagagtgca taacctgaaa aatgtgacgg tggaaatacc
     3241 ccgaaacaag ctgattgttg tcaccggtgt ctcaggttcc ggcaaatcat cactgacgat
     3301 cgatacgctg tatgcagagg ggcaacggcg gtatgccgaa agcctgagtg cctatgcccg
     3361 ccaatttctg aaccggatga ataaaccgga tgtggattat attaaagggc tttgcccggc
     3421 cattgccatt gaacagaaag tgatcacacg gacaccaagg agtacagtgg gaagcatgac
     3481 cgagatctat gattacctgc ggctgctttt cgccaggatc ggtaaaacga tctctccggt
     3541 ttccggaaaa gaagtaaaga aagatgatgt gacagatgtg ctggatgcga ttataaagtt
     3601 gaaagaaggg gacaaggttt atatactggc cacatttaaa caacacaaga accgggatat
     3661 aaaggaagaa ttgaatattc tggtacagaa aggattttca agagtctata gccgggaccc
     3721 gggagccggg aatgagggac agatatttag aattgaagag ttacttgaat taagtgataa
     3781 agaattgaag tccaaaaaaa tcactcatat ccttgttgat agaattgtag tgaaggaatt
     3841 tgatgaagat gataaacaca gaatggcaga ttctatcgga acagcattct atgaaggaga
     3901 aggagatgta tatattgaag taaacggaaa gaaagtagta aacttcaata accggttcga
     3961 actggatggc attcagtttg aagagcctgt tcccaatctt ttttctttta ataacccctt
     4021 tggggcctgt cctacctgcg aaggtttttc gcaggtgctg ggcattgatc ccgacctggt
     4081 aataccagac agaagattga gtgtgtatga tggtgcagta gccccctgga aaggagaaaa
     4141 aatggattgg tggcgtcagc aattcatcaa agtagccagg aaagtaaatt ttccggttca
     4201 taaaccaatt gcggatttaa cagataagca gtataaaaca ctctgggatg gtgtggatgg
     4261 aattggtatc agtgatttct ttaaagatgt ggagtcgcag ttgtataaag ttcagtaccg
     4321 ggtaatgtta agccgctatc ggggcagaac agaatgtcct gattgcaacg gttttcgtct
     4381 tcgcaaggaa gcactgtacg taaaagtaaa cggccggcat attggtgaac tgagtacaat
     4441 gccggtaaaa gatcttaaag tttggtttga tatactggcc aataaattaa gcagtcatga
     4501 taaggaagta ggaaaacgta tattgattga gctgcatggc cggctggata ccttattaaa
     4561 agttggcctg ggttatttaa cactcagccg cctggctaac tcgttaagtg gaggtgaaag
     4621 ccaacgtata caactgacaa gaagtcttgg cagtaacctc actaattcat tatatatttt
     4681 agatgaacct tctatcgggc tgcactcaag agatacggct aatctaataa aggttttaaa
     4741 agagctgaga gatcttggta atactgtggt ggtagtggag catgatgaaa tgatgatgcg
     4801 ggaggctgat catattattg atatgggtcc ttatgcatct catttgggtg gtgaagtggt
     4861 ggccgaagga gactatgatg atattatctc caatccaatg agcctgacgg gaaaatacct
     4921 gaagggagaa ttaaccattg atccgccaaa gactgttcgc aaatggaacc gttctgtaaa
     4981 agtggaagga gccaggcata ataatcttaa aaatatcacg gtagaatttc cgctgaatgt
     5041 attatgtgtg gtaagcggtg taagcggaag cggaaaaaca acattggtaa aacagatttt
     5101 ataccctgca ttaagaaaga tcaaaggaga gtttggggac aaggtgggat ttcacaaagc
     5161 aattactggt gatattgatg gaattggtca gttagaaatg gtggatcaga atcccattgg
     5221 caaatcatca agaagtaatc ctgttaccta tatcaaggct tatgatgaga taagggacct
     5281 gtttgccagc caaccgctta gtaagatgcg gggatttatg cccaaacatt tttcattcaa
     5341 cgttgatggc gggcggtgcg atacctgtaa aggggaaggg gaacaggtag tggaaatgca
     5401 attcctggca gatgtgcacc ttacctgtga atcatgcggg ggaaaaagat tcaaagaaga
     5461 agtgctggaa gtgaagtata gggataaaaa tatttttgat gtactggaaa tgagtgtgga
     5521 tgaagccatc aattttttct atgaggagaa aaatttggca aaagccattc agccattaag
     5581 cgatgtggga ttagggtatg taaaattggg ccagtcgtcc gatactttat caggaggtga
     5641 ggcacaacgg gtaaaactgg cttccttcct gggtaaaggc agggctaata ataagatcct
     5701 tttcattttt gatgagccaa ccaccgggtt gcattttcat gatatcaata aattattggc
     5761 atcattcaat gcattgattg aacaaggcca ttctattatc gttatcgaac acaatacaga
     5821 tgtgattcgt tgtgccgact gggtaattga cttgggcccg gaggcaggtg atggaggcgg
     5881 tgagttggtt tttgccggca aaccagctga cctgaaaaaa tctaaacaaa gtcacactgc
     5941 tgaatattta ttatagttgt gatgataaat tattgatccg tgataagttt tgatgggtaa
     6001 tgctttcgta gatctacgat tttttttatt gttataaaaa aagtaaatga taagcttgca
     6061 aaagctgaaa ctgcattata cctttacatt tactaagtaa aattcccatc cacatccttc
     6121 gaattccgta cctgacctca tctattaaga ttcactattt atttaaatga acagccagct
     6181 gctgtttact ataatcctat tcccaatctc ctcctgaaga gtccgcaata atcctttcca
     6241 tgcagagcca tactaattac gcacttttta aacttttaaa aactcgggca tgaaaaagat
     6301 ttattcatta atgatgttta cattgattgt ggtattattt actactaata atgtatttgg
     6361 acaaaatgaa ggcgattatc gctctgatca ggatgggaac tggaattctg tagtaacgtg
     6421 ggatgtttgg gatggagact cctgggaaaa tgcaacaacg attcagggtt atcctggtca
     6481 gtatagctcg ccagcttctg tgacaattag aaatggagat gatgttactt ttaatgttag
     6541 tcctgcattt agtattggag ctttggttat tgaggctgga ggttcgacga gtattttaga
     6601 ttttaattct ggtacaacat taaatgtttc aggagggatt acaattggtg ggggaactgg
     6661 aaatggggat gataagcgaa ttgatgttgg tgatggtata ttgacttgtg catcaatcac
     6721 catgttaaca actggtaatg acggagcaga cagcgaggta agaatcgatg atgggacagt
     6781 tacagtaagt ggtgatataa caatgaatga tgttgcgaca agaaatttta tcgaatttac
     6841 gaatccgggt actttgaatg tgggcggaag tatttctgct ggtggtgata ttgtcccaaa
     6901 taatgcaaca gaaggcttgg ttaattataa tggcaccagc ggaacacaaa cagtgaaatc
     6961 tgacacatac gcagatatta ctttttcagg gggtgccgta aaatctattg caacgggtgg
     7021 tattattatt acagatgttg caacttttac aaatggtatt gtgaattcat catctaccaa
     7081 ttcaatcaca tttaatgaca attcaacaac cacaggtgct aacgatgcat cttttgtaaa
     7141 tggtccaatt attaaagttg ggaatgatgc tttcgacttt cctgtcggca ctacaattgg
     7201 cggaaaccgt tatcacccca ttagtatcgg ggcgccatct gcatctacca cttttgaagc
     7261 ggaatatatc agggccagtt ctgtgttact ggaaccagat ctcattacag gtgtttatgc
     7321 tgttagtgcc tgtgaatact ggaggcttga cagaacaggt gctgctgcaa ctgccgatgt
     7381 taccttatac tggaatgaat acagcccctg cggcggagcc tatattacaa cgctgacagg
     7441 attaattgta gcctggagag atctagctga ttccagatgg gagaatagtt tgggtggccc
     7501 tcaaactatc acaagtgttt ctacttatac aaccggcaca cttctcagaa cttccgtaga
     7561 tgcaaatgga tttggttact ttacgtttgg cagtgccaat attaacggaa gcccgctacc
     7621 tgttaagctc agcaatatca gggcttttga aaagcagcag ggtatccaga tcgactggac
     7681 tgcttaccag gaggataacc tgaaccgtta ccaggttgaa cgttcagcag atggtcagtc
     7741 atttatgccg ataggcgacg tggcagcccg taacctcgtt attgaaagca actacagttt
     7801 ctttgatgcg aatccattac ccggtgtaaa tttctatcgt cttaaaaata ttgatatgga
     7861 cggtaagtcc ggctacagca atgtggtaaa agtgaacctt gacaaaaata ttaaaggctt
     7921 tacactctat cctaaccctg ttcggaatgg tttggtttcc ttccagtcct cagatctttc
     7981 gaaaggaaat tattcagtga atatatttaa tgcgaatggc cagcaggtgt attcacaaag
     8041 atttagtcat agtggtggct ctgtaaacca gacccttcaa ttacctgcag gtgtaagatc
     8101 aggaatgtac actatgcagg ttgacaatgg cacgatgaag ttgatgagta aaacttttat
     8161 ggttcaataa gaaattgcat aaccaggtta ttaaggctgc cttaaaaggc ggcctttttt
     8221 gttgctatgt ttttttgaga ggaatatctt catgtactaa ttagatctaa ataaaacttc
     8281 ttttaaaatt gagttatata taacttattg atatggcaga atgcatcgta tttgtacgag
     8341 tagtgattaa gtataagcac cctatttttt aaacataagt aagcaaaaac cattacaatt
     8401 tagtaaggtc atttacttta gctacttaga aaaatctaat ggtttttcat taaaatctaa
     8461 taactatttg taaagcctta gttgatgaaa attctgtttg taacagtttt ggcctttctt
     8521 tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcattt tcaataaaca
     8641 ggtcttacaa tactaatgca gcaaatgtta taacaactgt gtcaggaacc ctgcgtcttg
     8701 tttccaataa tggtacaata gttaatgcta ctccggcaaa actttttttt acggcaggca
     8761 gtacgtatga acattcccgc aatggcggaa caataccaac agctgcctgg aatgcagcct
     8821 cgaactgttt tgtaacggga attaccggaa cggctccaac ggggttgaat cagtcattcg
     8881 gaaatttttc ctgggattgt accggacaat caacgggatt tctattgaat ggggtgctaa
     8941 caactgtcaa tggaaacctt tccgtcggaa ataccaactt gtttggggtt tttctggcaa
     9001 ctgccagtaa cagctcttat acacttgata taggcggcga ttttataatt aatgataatg
     9061 cgtggctttc tattactaat ggtgataata taactgcaac tgttaatgta gctggcgatt
     9121 tcaatatgac agggataagt tcttcttcta cattttttga ttatcatgct gccaccggtg
     9181 gcgcagtcac tctgaataaa atcattatga atattgctgg tgattttata cagagcggcg
     9241 gcttgtttga ttttgcatat ggcgattcag atgcatcaaa ttttaccgag ctgcgtctgt
     9301 ctggtaattt gacaattagt aactccggcc tgatgcaaac gggatctgcg gataattcgg
     9361 tatcgaacgg aactatcatt ttcaataaac ccggtataca aactttcttt gccgcaacac
     9421 ctgctaacat tgcctatgta aactatgttg tgaataacgg gagtacactt gagatgctct
     9481 ctgatatcag tttgtccagt attgcaactg ctgtatgggc tggccagttt actgttaatg
     9541 ctggtgctgt tcttaatatt gattcaaaca ggattgttag ttcttcaggg gcaacagccg
     9601 gcgtaaataa ttcgtttata cttaacgacg gagcagttat tatttctgct aatcctgatg
     9661 gccttcagca taatgaaacg atcggttctg tttccacggc aattgctaca agaacttttc
     9721 gtagtaatgc tgattatgag tttaagggag ctgtaaccgg aacatttata actactccca
     9781 ttgaaaatac tgcgagagat attattataa ataataccag tggtaatgta acacttagtc
     9841 agcctatggc cgtcacaaga tcttttattc tggcttccgg taatttaatt actacaataa
     9901 caaatctgct tacaataaat gacgatgcta catcctcagc gggcagttat tctccggttc
     9961 ggtatgtaga tgggccggtg cgaaagatcg gggatgacac tttcaccttt ccagttggaa
    10021 aatctggtgt gtatgcgcca atcagtattt cggcaccggg aactgtgaat gatgaattcc
    10081 tggcagagta tatgcgtaca actcctccaa acagattttc aataacggca ccaggattaa
    10141 atcatattag tttttgcgaa tactggaacc tggaagaaat tggaccgggt agtcccaccg
    10201 ttaatgtatc gttgtcatgg agcggattga gtccctgtaa tacggctgca tacataaacg
    10261 atttagcaac gctgacagtg gcacaatata atggcaccag ctgggacagc catggaaata
    10321 gcggtggcac taccgggtct ggcagtttag gtactgtaac ccgtaataat gtatctgtat
    10381 ttactccatt ttctattggc agcaccagtg ccggaacaaa cccgttaacg gttaagtttt
    10441 ctttagtaaa agcttacccg gctgggaata tcaataaaat tgaatggacc aacagttcag
    10501 aagaaggagt tgctcaatat gaggtggaaa gatcgacaaa tggtatttca tttgttatga
    10561 ttaaaaatat aaatgcccgg agtaatgccg gtacaagaga agattatgtt gtaagcgatg
    10621 aaaatccgta cccggatatt tctttttatc gtattaaggc ggtagaaata aatggtgtga
    10681 tttcttatag ccctgttata aaaattatcc gaaatacaac tgcagatatg aagctgcagg
    10741 tatatcctaa tcctgttgta aaccggcagt ttgtaataca gttgcgaaat cagcaaagag
    10801 ggaactatct cttgaaatta tttaataaca gtggtcagga ggtatatcaa acaaactggc
    10861 agtttgccgg aggttctgca tcaaggacca ttgaattgcc atcaggaatt attcccggaa
    10921 tgtaccatgt acaattgtcg gcaaatgaat caatgctgca atccaaaatt attatcaggt
    10981 aagtaaatgt ggtttgataa tagcaacggc tgtcagtagg gacagccgtt gctatttata
    11041 aaaatgtaat cttctgatta acgaagttta tcgaggcttt ttaccagttt ttcatcttta
    11101 cggatgttac gaaatgccat gaaataacca atcaggataa ataaaggaag tactgaagta
    11161 agcgccggaa ttggttttat gagtttattc atttccagga catagagaaa gatcaaaagg
    11221 ccagccacca ggattcccag caggcaaagc caaatctgct gtttgcggtt tttaaaaaga
    11281 aaaataatta cagtagataa gattaacgat cccccggtca gcaacagcag gaaaaaatta
    11341 ctgccagcat ctatcaccgt gcggacaggg ctggggctgt tttgttgcat ctcatcgcca
    11401 accacaaatg gaaacatgaa actaaaaatg gctgctgcag ttgataataa caaccagagt
    11461 gtttgctgac gttgaatcat aacttactat ttagcgtaac aataattgaa actacaacct
    11521 tatcccaact ccggatataa tgggaattgc tgcataaatg ctttcacttc acttttcact
    11581 gcagctatta cctcttcatt gtctgcattc atcaatactt tgtctattaa ggaaacaact
    11641 gtttccatat gtccttcttg caggcccctg gtggtgatag caggtacacc cacccgtatt
    11701 ccagaagtaa caaaaggtga tttatcatca aaaggtacag catttttatt gagagtgata
    11761 tgggctttgt ccagtgtttc ctgtgctttt ttgccggtca ggtttttatt gcgcaggtca
    11821 atcagcatta aatgattgtc tgttccatta ctgatgagtt ggtaacccct gtttacaaat
    11881 gcttttgcca ttgcctgagc attggcgatt acctgctggc cgtaagcctt aaaatcttcc
    11941 gtcaggattt caccaaatgc cacggctttt gcagcaatta catgctccag cggcccgccc
    12001 tgagaaccag ggaaaacagc gagatcgagc aactgactca tcgtccttaa atttcctttt
    12061 tgatctttta taccccaggg attttcaaaa tcgtggcgca gcatgatgat gccgcccctg
    12121 ggtccacgca atgttttatg tgttgtagag gaaacaatat ggcaatggtc gaacggatcg
    12181 ttcaataatc ccgatgcgat aagaccagcc ggatgagcaa tatctgcaaa tacgatagct
    12241 ccaacctcat cagctactgc acggattcgt ttgtaatccc agtcgcggct gtaagcagaa
    12301 gcaccgcaga tgatcatctt gggtttttct gcacgggcct ttgcttccag ttgttcataa
    12361 tctacaatac cgtctttggt tacaccataa tgaatagcat ggtatgtttt accactgaag
    12421 ttagccgggc ttccgtgtgt aaggtgtcca cccatactta ggtcaaggcc gatgaatttt
    12481 tctccaggct tcattacagc caaaaaaacg gccgcattgg cggatgcacc actatgtggc
    12541 tgcacattgg cccaactggc attaaatact ttttttaatc tttctatagc gagattttca
    12601 atttcatcca ctacttcaca accgccataa taacgtttgc ctggatagcc ttcggcatat
    12661 ttgttagtag gtacagttcc catcgcctgc attacctgca gcgaagtaaa attttcagag
    12721 gcgatcagtt caattccatt tcgctggcgg tccagctctt tgcgtatcag ttcgaaaaca
    12781 agggtatctt tttgcatgcc gcaaaattaa gtgagtagaa ggaatgaaca gcggcattct
    12841 gtacaggatt ttgtttacgc taaaagtgtt actttcgttg gcttttgatt agttaccccg
    12901 aattaacctt cggaaaaacc attttggctc atacatgaaa gctattatac cagtggcggg
    12961 tgcaggcact aaactgcgac cgcatagtta tacacaacct aaggcactca ttccccttgc
    13021 aggaaagacg atactaagca ttattattga ccaattaaaa gaagcgggta ttgatgagtt
    13081 tatttttatt gttgggtacc tgggtgaaaa gattgaagat tatgtaaagc tcaaatatcc
    13141 tgacctggtg gctcattatg tacaccaaac agaccggcag ggggttggtc atgctgtgcg
    13201 tcttaccagg aacctggtaa accaggacga ggtttttgtt gtattgggcg atacgatctg
    13261 cgaatatgat gtgaaggaag tggtgaacag tccggattcc atgattggcg ttcgcaaagt
    13321 ggatgacccc cgtgattttg gtgtggcgga aatcaatgag gactgttata ttgaacatgt
    13381 cgtggagaaa ccccagatac cgaaatcgaa tatggcgatg gtgggtattt ataaaataaa
    13441 agaaagcgaa cagcttttcc aatgcctgga aaataatatc cggcagggac tccgcagcca
    13501 tggcgaatac agcctgactg atgcacttga ttgtatgatc aaagcagggg ctaaattcaa
    13561 atcattcaag gtagagaact ggtttgactg cggacgtaaa gaaacgcttt tggaatccaa
    13621 cgccttactg cttaaaaaat ttgcccctca aactgatgca ggacggtttg aaaatacagt
    13681 gatcgttcct ccggtaagta ttggtgaggg ttgtactatt aaaaattcca ttatcggacc
    13741 caatgttacc attggagaaa atactacagt agattattct attgttaaag actccattat
    13801 aggttccttt tccaatctct ttgatattgt gctggacgac tcagttatcg gaagtgatac
    13861 caatcttcac ggggaatccc gttcgctgaa tatcggggat aatacaagta ttgatttggg
    13921 atagtgtagc agtctggaga catgcgaatc ggatcagtag tgttgaccgg tgcattagtt
    13981 atctttactc ctgattaatg atttttaacc catgactcgt ttctccaatc gtattaccca
    14041 actcttcaac atcgactttc ccattattca ggccggtatg atctgggcca gcggttggcg
    14101 gcttgccagt gcagtgagta atgccggagg ccttggcatc attggtgccg gttcgatgta
    14161 tcctgaagta ttaaaagaac atattcagaa atgtaaaaca gctaccaatc gtccttttgg
    14221 ggtcaatctg cccttactat atcctgatat tgataagcat atccaaatta ttattgaaga
    14281 agaggtaaag attgttttta catctgccgg aaatcccaaa acatggacca gcctgttaaa
    14341 agaaaaagga ataacagtgg tacatgtggt cagttcttca aagtttgcca aaaaagcgga
    14401 ggatgccgga tgtgatgcag tggtggcgga gggttttgaa gccgggggcc acaatggaaa
    14461 agaagaaaca acaacattgg ttttgattcc ggcggtggtg aatgcagtaa agattccggt
    14521 aattgctgcc ggcggcattg ctaccggaag acaaatgctg gcagcaatgg tgctgggggc
    14581 agaaggtgtg cagatgggaa gccgttttgt agcctgcgaa gaagcttcgt ctcatattaa
    14641 ttttaaaaac gtggtgatca actcgcagga gggggataca caactggcat tgaaacaatt
    14701 aaccccggtc cgtttgataa aaaatgattt tttcaggcag gttcaacaag cagaacaacg
    14761 aggggcaacg gtagaagaac tgagtacgat attgggaagg gcaagggcaa agaaggggat
    14821 gtttgaaggt gatttacagg aaggagagtt ggagattg
//
LOCUS       DAORTI010000087        14609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_3948_length_14609_cov_6.172667, whole genome shotgun sequence.
ACCESSION   DAORTI010000087 DAORTI010000000
VERSION     DAORTI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14609
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_14609_cov_6.172667"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLO70_11250"
     CDS             complement(<1..869)
                     /locus_tag="PLO70_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75087.1"
                     /translation="MTRFIYFLLIITALNPLATLAQNKPARQVNMAELESKLILPQWA
                     SVMSGHTRNNTTGNFTTPDASCDTILLTRQSQIDSFKILYPGCTSVQLLEVNGQGASP
                     AITQLDSLNEIESVSQKLKISYTSITSLSELASLTSLGDTLELQYNFLQTSIGLNNLT
                     NLGTIIFNKLPVLNTLAGLSNGITTIGGVLIDSTALTSLTGLESVETINGYLDLRYSP
                     IVSLSSLTNLDSIIGYLRLETLPSLTSISLNNLEYVYGFLFAGLNNLTSLAGLSDNLL
                     TTNLGTIWMINTGL"
     gene            1074..3047
                     /locus_tag="PLO70_11255"
     CDS             1074..3047
                     /locus_tag="PLO70_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75088.1"
                     /translation="MCYRKYHIVLIITLFPVFVNAQTRTIDSLRKKLWVSEKESYRLG
                     ILLSLCEEYQSLNRDTLYAYAIEARDLAAAQKNKKQVSLAALALANAYMQWGWVDSAL
                     ASIEPEIPLNPVTDPTTRDTYFKLTRQKAMCYGNKSDFEKALAIFFPLLSEAEIYKDT
                     LNMGGIMNTVGSIAIARGEPAEALKWINKAITFSIAGPKYFPVLAAVYVNAANAYNQI
                     EKTDSAFYFIQKSIPICRKLENLNYLATALRVQTSLMTKKGRLDEAEASLKEMISIRR
                     LTSVGEFFVDDNLQLADFYANTGQLDKAIAFCKKQLQTGNVYDTAAKESATFTNDPKM
                     RLAYFQALSKYYKQAGMYPEYQETLEQIIAAKDSFYVANSAQAIADAEAKYEVQKKEN
                     TILQQKYGLQRKNFFIYGAFGLLALALITVYFIFRDYRRRQRLKMTLALAEEKRITIN
                     AVKAAEEKERVRIAADLHDNLGAYAASLSSNLGYIQPLDKESAMSNAFRELNNNSNAI
                     ISELNDTIWVLKKEALSLTAISDRIKVFISRIQKSYPDLHIEVEEQVKTDHQLPSSQA
                     FHLYRVLQEAINNALKHSKGKNIKVKITGDNNWNVSVADDGTGIDLSNESAGGGGNGL
                     NNMKERCKEAGWEISWQKTDSGGTIVKISPTTN"
     gene            3089..3763
                     /locus_tag="PLO70_11260"
     CDS             3089..3763
                     /locus_tag="PLO70_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75089.1"
                     /translation="MNFIIALTEDNNINRNTFLQKMNLLGNMQLAFVAANGNECLEQL
                     KGMPQSKLPQVIFMDLEMPQMDGIETIRLAKSLHPQIHFIVLTVFDDDEKIFEAIKSG
                     ASGYLLKHEPAGVLQEAIMNVLEYGGAPMSPAIARKALQLMSRSSIDASLSKKNPLPE
                     MITTREEEILRHMVNGWDAKRIASELDISVLTVRKHIANVYEKLHVQSKAEIISMAHQ
                     NKWFNH"
     gene            complement(3760..4209)
                     /locus_tag="PLO70_11265"
     CDS             complement(3760..4209)
                     /locus_tag="PLO70_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ75090.1"
                     /translation="MSIQVRRAVKKDCPRLLELVKELALYEKAPQEVTVTAEHFEESG
                     FGSNPVWWAFVAEENGVIQGFALYYIRYSTWKGQTMYLEDILVTEAARGKGIGKLLMD
                     QLIIEAIEKGFSRIVWQVLEWNEPAINFYKKYNADFDAEWVNCSINV"
     gene            complement(4214..6031)
                     /locus_tag="PLO70_11270"
     CDS             complement(4214..6031)
                     /locus_tag="PLO70_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQZ75091.1"
                     /translation="MKQLLTFLLLLACSFSFAQKKNKPANDPFAGLDTAFQRVLKDRK
                     AAGFAVAVVSKDKVLYAKGFGYRDYENKLPVTPNTLFAIGSCTKAFTSSLMGLLNKEN
                     KLEYDKPARDYLPTLHFFNDNLTNMVTVRDMMCHRTGLPRHDYSWYLFNSSSRDSLLE
                     RIKYHEPTVPIRQAWQYNNFMFLAQGMIAEKLTGKSWETNIKERIFEPLGMLSSNLSI
                     HDLKKNKEAALGYRVYKDSLIRKTDYYDINAMGPAGSINSNVTDMANWVMTWLNGGKF
                     NGKEILPASYVKEAMTSQMVISGALPDKEIPDAHLANYGFGWMISSYRGHYRVEHGGN
                     IDGFSASTCFFPSDSIGIIVLTNQNGSALTGIVRNLIADRLLDLKTIDWNGRTNAAQQ
                     KAKVAEKEAEKTVTSNKKEGTKPSQERKNYLGLFNHPGYGTFEIINRKDSMIMLTQNG
                     DAWLRHYHYDIFEVLSIDKTEGIDTTSKDNLKIQFQMNAAGEITSVLIPLQPGLKDLE
                     FSKKPKAKELTKEELTKYTGEFEFAPGNAVKFYLKGEKVLYAFIEGQPEYELMPVDKN
                     KFALKILQGYSVQFEENEKGEIIAASFIQPNGTFKAKKK"
     gene            complement(6076..>7293)
                     /locus_tag="PLO70_11275"
     CDS             complement(6076..>7293)
                     /locus_tag="PLO70_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQZ75092.1"
                     /translation="SIMDKSFPLTGWGSLAVHAGHKQDPNYAHLVPIYASSTYVFDDA
                     EQGMRRFSGKEEGYIYSRWGNPTITEAEEKIAALETFGLGLEAKGILHASGMAAISTL
                     MLSALKPGDKIITHYSLYGGTEELVSKVLPAFGITAIIADLRDLQKAEDIIKAEKSIK
                     MMYLETPANPTIQCVDLDELSKLAKQHNLVVACDNTFATPYLQQPFQYGADFIVHSTT
                     KFLNGHGTAIGGILLGRDIEQMKTRITKIHRTLGGNANAFDAFLLIQGMKTLEVRMER
                     HCHNAMEVANYLENHDAVSKVNYTGLTSHPDHYTAMKQMRHPGAMLSFELKGGLQSGI
                     NFMNNLKMCVRTVSLGTCDTLLSHPASMTHYSVPKEEKEKYGITDGLIRMNVGIENLR
                     DIISDLDQALHTA"
     assembly_gap    7294..7393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7394..7687
                     /locus_tag="PLO70_11280"
     CDS             <7394..7687
                     /locus_tag="PLO70_11280"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQZ75093.1"
                     /translation="RPSDNPKDSTRDVINGLLSQSKLWEGWVSNASELSLDHVFQFNN
                     KKGEPVKMYIYQMLVHVFNHSTYHRGQLINMLRQLGIEKVPTTDFALWVKNKK"
     gene            complement(7782..8084)
                     /locus_tag="PLO70_11285"
     CDS             complement(7782..8084)
                     /locus_tag="PLO70_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ75094.1"
                     /translation="MLKKNKTLTLDQFKEKHYGKRGSAKRENLEKGYQEFKLGALIYE
                     ARLEKGMTQEELAEKCGTNKAYISRVENDIKDVRISTLQKIIEVGLGGQLNLSIKL"
     gene            complement(8068..8418)
                     /locus_tag="PLO70_11290"
     CDS             complement(8068..8418)
                     /locus_tag="PLO70_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQZ75095.1"
                     /translation="MPENDKNRTIIFYKNYFEEFFVKQRQKVKDKIIWTFELIEDLPQ
                     VPETYLKHLEGTDGLYEIRVQQGSDIVRIFCFFDKGKLIVVTSGFQKKSQKTPRQEME
                     KALKIKEEYYAEKK"
     gene            8523..10613
                     /locus_tag="PLO70_11295"
     CDS             8523..10613
                     /locus_tag="PLO70_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ75096.1"
                     /translation="MGRPTKAKNSDSLQEQKDMGGDGWQQTTYGRVSRDVYGFKLGYF
                     SNDYTPIGGATAGAFGNKTYSAPGSLDQTGNELFNGNISFTTLALSKLVGAATTGYSY
                     GYDQLNRLVEMRQHSTGTTSGWSNSSIVTAYRESISYDANGNILKYLRKGHSATPHMD
                     SLTYQYNYSSGKLINNRLNHVSDSIPAGNYSEDIDDQDAGNYQYDYIGNLRADGAEKI
                     DTIRWTVYGKINRIVKSEDNVSIDYSYDAAGNRTRKRVNNNDTISITWYVRDAQGNVM
                     AVYSNKEGTEDIVWKEQHLYGSSRLGMWRADTTVPALPPVVDEDPVYDSVMLGSRTYE
                     LTNHLGNVLATISDKKIGNDSSDVVNYYIAEVLSQTDYYPGGMLMPDRKYSAGTGYRY
                     GFNGKEKDTEGPVQYDYGFRIYDPRLVRFKSVDPLTRSYPELTPYQFASNRPIEAIDL
                     DGLEAKDLYMELEPVRQQRRMEQSFGIKHDETPAAKAVGIFVQVSLGATVLAPILVEA
                     PALATSTFSRLFWWAAANPETAAGVLYTIVVAASGYEGPDLPGPADDFGKWGRKVFDK
                     LTAVGVKSEKSLAQAARNSYNAIINTIDNEIKDIATIEQKARKAFDVRNKAKDFAREL
                     SGPANRAKAEAQSLEKHGNINGPSFDDLYKKNYDDAMKSGLKEEEAKNKAYQGIIESS
                     KRPDQETNKKYGAN"
     gene            10600..10911
                     /locus_tag="PLO70_11300"
     CDS             10600..10911
                     /locus_tag="PLO70_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75097.1"
                     /translation="MEQTDNMQLVKKNNHYELVVLESAVFEDYENILNYLMKEMNLKD
                     VEEIDDFDSRYQLFNYNEQIIVLYYSSFLGISIYFDDKTIDSGVQLKVLKEFWEVLKK
                     S"
     gene            complement(10997..11326)
                     /locus_tag="PLO70_11305"
     CDS             complement(10997..11326)
                     /locus_tag="PLO70_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75098.1"
                     /translation="MIVNVLKEADAISYTSKYFSSDSDLMISTKKILSLLKQEVINNP
                     ERINQRILRTMHDIWMSSYKDFDNTPMEQAINNVTEILFDELPCYKTLTRYEGILVKG
                     IQLKCKR"
     gene            11498..12550
                     /locus_tag="PLO70_11310"
     CDS             11498..12550
                     /locus_tag="PLO70_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75099.1"
                     /translation="MPGRSYTIGNGYRYSINGQENTPEIAPNTTTAEFWQYDARIVRR
                     WNVDPILKEYESPYATFGGNPIINIDPDGSDTINITRKTTFDKRQYRPPSISGNPNSH
                     NRVYDPGNDGITRSGSIEIMEAAGQDIFRITDLHIHIDENGNESTVSSTTTTLELSKI
                     SAGRIVAGRNVKGYADDRYALAAHAPRWLLEYYEKKTGGDIGIRSAIAYQKTVPFAHG
                     LGRITNIAYTVAGVYGLARGFLGRNAAGIGFNTFNEFKTAYGTAGIGKAWHHIVEQNP
                     ANIAKFGAQRIHNSTNLIKLDHGAGTIHAKVSGYYSSIQPFTNGQTVRKWLSSQSYQQ
                     QYDFGIKTMKQFGWTP"
     gene            12547..12891
                     /locus_tag="PLO70_11315"
     CDS             12547..12891
                     /locus_tag="PLO70_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQZ75100.1"
                     /translation="MKIKDKKTALASFEESAVKHADATGQGDYKTANKCYANIVKAIN
                     FLKEQGEVAALIDCLCHSSAGVRNWAATYLLPVKEKEAIKVLEEIVRKKDIHSLSAET
                     TLSEWRKGNLKL"
     gene            12923..13225
                     /locus_tag="PLO70_11320"
     CDS             12923..13225
                     /locus_tag="PLO70_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75101.1"
                     /translation="MFKYLLANLSDTPKEVNFKDISEFDRMEQRFFAIEQYYGDIVGM
                     NVDSVEFCPNHNPPELQNEILPWLWLIHPELKDQISEIADSQLRQIIREYRNHKEP"
     gene            13411..14418
                     /locus_tag="PLO70_11325"
     CDS             13411..14418
                     /locus_tag="PLO70_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ75102.1"
                     /translation="MPDRQYSAATGYRYGFNGKEKDTESPVQYDYGFRIYDPRLVRFK
                     SVDPLSKNYPSWSPYPFAMNRPIDGIDLDGLEYLKANESKIDIYVSYLEFNGRKVIYG
                     SVHLDMNNVSANTQNYVNNSPWKNGAIGSGRSKIATFEHIDYNDILAKKQEAFNVLFK
                     QADATDMEAKSLVQPNLEVPRIPKNKLEERQIQKSKQFTLPSGGGSSAKGNAIVAAVQ
                     LVGEVLQYWHGKKVDNDYNTANSQLASGQFALNDLQFALDNNLIPSQYLGNVSEFANY
                     LLDGKKPTITYYIKNDKGENVPKESVNNNLIKAFNEIKSNISKERAKLSEPKQCVPCE
                     E"
BASE COUNT         4436 a         2714 c         3084 g         4275 t
ORIGIN      
        1 aggccagtat tgatcatcca gatagtgcca agattggtgg taaggagatt atcgcttaag
       61 cctgcaagcg atgtaaggtt gtttaacccg gcaaacaaaa agccatatac atattcaaga
      121 ttattcagac taatagatgt tagtgaagga agagtttcaa gtcttaaata accaataatt
      181 gaatcaagat ttgtaagact gcttaaactt acaatcggag agtagcgtaa gtcgagataa
      241 ccatttatag tttctacgct ttcaaggccg gtcaaacttg ttaatgcggt actgtcgatc
      301 agcactccgc caatggtggt gatgccattg ctcaggcctg ccagggtatt taatacggga
      361 agtttattaa agataatagt accaaggttt gtcaggttgt ttaagcctat actggtttga
      421 agaaagttat attgcaattc cagcgtatct cctaaagaag tcagtgatgc aagttcgcta
      481 agtgaagtaa tagaggtgta tgagattttg agtttttggg aaacactttc tatttcgttc
      541 aatgaatcaa gctgtgttat ggcagggctt gctccctgtc cgttaacttc taaaagctga
      601 acagatgtac atccgggata tagaattttg aaactatcaa tttgtgattg acgggtaagt
      661 aaaatggtat cgcagcttgc atccggggta gtaaaattgc cagtagtatt gttcctggta
      721 tggccgctca taacagatgc ccactgagga agtatcagtt tactctccaa ttcagccata
      781 ttcacttgtc ttgcaggctt gttttgggcc agggtcgcca atggatttaa agctgtaatt
      841 atcaggagaa agtaaatgaa tctggtcatc agtcagtttt ttagtgtctt tttaaactgc
      901 atgtagcagg ataataattc tgtgatttat cagtaacagg ggccggcagc ttattagagg
      961 ttggatttcg acactataaa aattgaattt aaccaggcag cagaaaatac ttaatttgta
     1021 gtaggctccc ttaaatagtc tcggtttatt ttatttttag gtctgcaacc tcaatgtgtt
     1081 atagaaaata tcatattgtc ctgattatta ccctcttccc ggtctttgtt aatgcacaga
     1141 cccgtacaat agatagcctg agaaagaaac tttgggtatc cgaaaaggaa agctaccgtc
     1201 tcggtattct actttcttta tgcgaagaat accaaagcct gaacagggat acattgtacg
     1261 catacgcaat tgaggcaagg gatctggcag cagcacaaaa aaataaaaaa caagtgagcc
     1321 ttgccgcact ggctctggct aacgcatata tgcaatgggg ctgggtggat agtgcactgg
     1381 cttctattga acccgaaatc ccgctgaatc ctgttacaga tccgaccaca agggatactt
     1441 attttaagtt aacccgtcaa aaggccatgt gttatggcaa taaatctgat tttgaaaaag
     1501 cgctggctat attctttccc ttattgagtg aggctgaaat atataaggat acattaaata
     1561 tgggaggtat tatgaatacc gttggctcca ttgccattgc caggggagaa cctgcagaag
     1621 cactgaaatg gattaataag gcaattacat tttcaatagc aggccccaaa tatttccctg
     1681 tattagcggc cgtatatgta aatgcagcta atgcctataa ccaaatagag aaaaccgact
     1741 cagcatttta ttttatacaa aagtcaatac caatttgcag aaagctggaa aacctgaatt
     1801 atctcgccac tgccttacgg gtgcaaacca gcctgatgac caagaaaggc agactagatg
     1861 aagcggaagc atcactcaag gaaatgatta gtataagaag attaaccagc gttggtgaat
     1921 tttttgtaga tgataatctg caactggcag atttttatgc taataccggg cagttggata
     1981 aggctattgc tttttgcaaa aagcaactgc aaacaggaaa tgtttatgat actgctgcga
     2041 aagagtcggc aacttttact aacgacccca agatgagatt agcttatttc caggccttgt
     2101 caaaatatta taaacaggcc gggatgtacc ctgagtacca ggaaacactg gaacagatta
     2161 ttgcagccaa agattcgttc tatgtagcca attcggccca ggctatcgct gatgcggagg
     2221 caaagtacga ggtacagaaa aaagaaaata cgattctgca acaaaagtat ggcttgcaac
     2281 gtaagaattt cttcatctat ggtgcatttg gtttactggc actggcgctt attactgttt
     2341 actttatttt cagggattat cgccgcaggc agcggttgaa aatgacattg gctttggctg
     2401 aagaaaagag aattactatt aatgcggtaa aagcagcaga agaaaaagaa cgggtaagaa
     2461 ttgcggcaga tttgcatgat aatctcgggg cttatgctgc ctctctttct tctaatcttg
     2521 gatacataca gcctttggat aaagagtcgg ccatgagcaa tgctttcagg gaattaaata
     2581 ataactccaa tgctatcatt tcggaattga atgatacaat ctgggtgttg aaaaaagaag
     2641 ctttatccct gacggcaatc agcgacagga taaaagtatt tataagtcgt attcagaaaa
     2701 gctacccgga cttgcatata gaggttgaag aacaggttaa aaccgatcac caattgcctt
     2761 cttcgcaggc atttcacctc taccgggttt tacaggaagc tatcaataat gcactaaagc
     2821 atagtaaggg gaaaaatata aaagtgaaaa taaccggtga taacaattgg aatgtttctg
     2881 tagcagatga cggcaccgga atagatttga gtaatgagtc tgcaggcggt ggaggaaatg
     2941 gattaaacaa tatgaaggaa agatgcaaag aggcgggatg ggaaatttcc tggcaaaaga
     3001 cagattcggg cgggactata gttaaaattt ctcctactac aaattgatta tttttcagcg
     3061 tctgccgatt cctaatttta atatacatat gaacttcatt atagccctta cagaggataa
     3121 caatatcaac aggaatacat ttctgcaaaa gatgaatttg ctaggcaata tgcaactggc
     3181 ttttgttgct gctaatggta acgaatgcct ggagcagtta aaaggaatgc cacagtcaaa
     3241 attaccacag gtgatattta tggatctcga gatgccgcag atggatggta ttgaaactat
     3301 tcgccttgca aagtcactgc atccgcagat acattttatt gtgctgactg tttttgatga
     3361 tgatgaaaaa atttttgaag ccattaaatc cggggcctcg ggctaccttt taaaacatga
     3421 gcctgccggc gtattacagg aagcaataat gaatgtactg gaatatggtg gtgcgccgat
     3481 gagtccggca atagccagga aagcactgca attaatgagc aggtcatcaa ttgatgcttc
     3541 tttatcaaag aagaatccgt tgcctgaaat gattactacc cgggaagaag aaattctcag
     3601 gcatatggta aatggctggg atgccaagcg gattgcgtca gaacttgata taagtgtatt
     3661 aacggtccgg aaacatattg caaatgtcta tgagaaactg catgttcagt caaaggcaga
     3721 aattatcagt atggcgcacc agaacaaatg gttcaatcat taaacattga tcgaacaatt
     3781 gacccactct gcatcaaagt cagcattgta ttttttataa aaattaatag caggttcgtt
     3841 ccattccagc acttgccata caatccggga aaatcctttt tctattgctt ctattatcaa
     3901 ctggtccatt aataatttac cgataccttt tccccgggca gcttcagtta ctaatatatc
     3961 ttcaaggtac atagtctgcc ctttccaggt tgaatagcgg atataatata acgcaaaccc
     4021 ttgtatcacc ccattctctt ccgcaacaaa tgcccaccac accggattgc tgccaaaccc
     4081 actttcttca aaatgttctg cagttacggt tacttcctgg ggggcttttt catagagtgc
     4141 cagctccttt actaattcca gtaacctggg acaatctttt ttaacggccc tcctgacttg
     4201 aatactcata ctattatttc tttttagctt taaaggttcc gtttggctga atgaaactgg
     4261 cggcaatgat ttctcctttt tcattttctt caaactggac actatagccc tgtaatattt
     4321 ttaaagcgaa tttgttttta tcaactggca ttaattcata ctctggttgc ccttctataa
     4381 atgcatacag aactttctct ccttttaaat agaatttgac agcattgccc ggagcgaatt
     4441 cgaattcacc ggtatatttt gttaattcct cctttgtcag ttctttggct ttcggttttt
     4501 ttgaaaattc aagatctttt aaacctggtt gcaggggaat tagcacagaa gttatttcgc
     4561 cggctgcatt catctggaac tgtattttca ggttatcctt actggtggta tcgatacctt
     4621 ctgttttgtc tattgataat acttcaaaaa tatcatagtg gtaatgacgc agccaggcat
     4681 cgccattttg tgtaagcatg atcattgaat cttttctgtt gatgatctca aatgttccgt
     4741 atcccgggtg attgaaaagg ccgaggtaat tttttctttc ctgtgatggt ttggtgcctt
     4801 cttttttatt gctggttacc gttttttcag cctctttttc agctacttta gctttttgct
     4861 gggcagcatt ggtacggcca ttccagtcga ttgtttttag atcaagtagc ctgtctgcaa
     4921 tcaaatttct tacaatacct gttaaagctg atccgttttg attggtcaga acaatgatac
     4981 cgatgctgtc agacgggaaa aagcaggtac tggcagagaa cccatcaata ttaccaccat
     5041 gctctacccg gtaatgtccc ctgtaggagg agatcatcca gccaaatcca tagttggcaa
     5101 gatgagcatc aggtatttct ttatccggaa gtgctccact aatgaccatt tgtgaagtca
     5161 tggcctcttt cacataagag gcaggtaaaa tttccttgcc gttaaatttt cctccgttga
     5221 gccaggtcat cacccagttg gccatatctg tcacattgct gttgatgcta ccggccggtc
     5281 ccatggcatt gatgtcataa taatcagttt ttcggataag gctgtcttta tatacccggt
     5341 atcctaatgc ggcttcttta ttttttttca gatcatggat agaaagatta gaagacagca
     5401 tacccaatgg ttcgaatatt ctttctttaa tatttgtttc ccatgatttt cctgtcagtt
     5461 tttcagcgat catcccctgt gccagaaaca taaaattatt gtattgccat gcctggcgga
     5521 taggaacggt gggttcgtgg tattttattc gttccaataa actgtctctt gatgaactgt
     5581 tgaacaaata ccaggaataa tcatgccggg gtaggcccgt acgatggcac atcatgtctc
     5641 tcaccgttac catattagtc aggttatcat tgaagaaatg aagtgtcggc aaataatccc
     5701 ttgccggttt atcatattcg agtttgtttt ctttattaag cagccccatc agcgatgagg
     5761 tgaatgcttt ggtgcaggaa ccaattgcaa ataaagtatt cggagtaaca ggtagtttat
     5821 tctcataatc acggtatccg aatccttttg catacaacac tttgtctttg ctgacaaccg
     5881 ctaccgcaaa acctgctgcc ttcctgtctt ttaataccct ttgaaaagca gtatccagtc
     5941 ctgcaaaagg atcgtttgcc ggtttatttt tcttttgggc aaaagaaaaa ctacaggcca
     6001 gcaaaagcag aaaagtaaga agttgtttca tatatattat gtttaagtgt gaatgctatt
     6061 taagtacagc tggtattatg ctgtgtgtaa agcctggtca agatcactga taatgtcccg
     6121 caagttctca atacctacat tcatccgtat taagccgtcg gtaataccat atttttcttt
     6181 ttcttctttc ggtacgctgt agtgtgtcat cgaagcaggg tgggagagca aggtatcaca
     6241 agtacctaat gaaactgttc gtacacacat ttttaaatta ttcataaaat taataccgct
     6301 ctgtaatccg cctttcaatt caaaactcag catagctccc gggtgccgca tttgtttcat
     6361 tgcagtgtaa tgatccgggt gacttgtcag cccggtgtaa tttacttttg aaacagcatc
     6421 gtggttctct aagtaatttg caacttccat tgcattgtgg caatgccgtt ccattcttac
     6481 ttcaagcgtc ttcatgccct gtatcagtaa aaatgcgtca aatgcattgg cgttgccacc
     6541 cagtgtacgg tgaattttgg tgatccttgt tttcatttgt tctatatccc tgccaagcaa
     6601 aatacctcct atcgcggtgc catgtccgtt taaaaatttg gtagtggagt gaacaataaa
     6661 atcagcaccg tattgaaagg gttgttgtaa ataaggtgta gcgaatgtat tgtcacaggc
     6721 tacaacaagg ttatgctgtt ttgccagctt actcaattca tccagatcca cacattgaat
     6781 ggttgggtta gccggagttt ccaggtacat catcttaatg gatttttccg ctttgatgat
     6841 gtcttccgct ttttgcagat ctctcaggtc tgcaatgata gccgttatgc cgaaggcagg
     6901 caggactttg gagaccagtt cttcggttcc cccatataac gaataatgcg taatgatttt
     6961 atcaccgggt tttaaagcag acaacataag ggttgaaata gcagccatgc cggatgcatg
     7021 aagaatacct ttcgcttcca aacccaaacc aaacgtttcc agagcagcta ttttttcttc
     7081 ggcctctgta atagtcgggt tgccccagcg gctgtagata taaccttctt ctttcccgct
     7141 gaagcggcgc ataccctgct ctgcatcgtc aaacacatag gtactgctgg catagatcgg
     7201 taccaggtgt gcatagttcg gatcttgttt gtgcccggca tgtacagcca gcgatcccca
     7261 gccagttagt ggaaaacttt tatccattat agannnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnncgaccga gcgataaccc gaaagactct acccgggatg tgatcaatgg
     7441 tctgctgagc cagtcaaagt tatgggaggg gtgggtctcc aatgcttccg aattgtcact
     7501 ggatcatgta tttcaattta acaacaaaaa aggggagccg gtaaaaatgt atatctacca
     7561 gatgctggtt catgtattta atcacagtac ttatcacagg gggcaactga ttaacatgct
     7621 gcgacagctt gggatagaga aagtacctac aactgatttt gcattgtggg ttaaaaataa
     7681 gaaatagggg attggcgttt tgcggccagc gttcccgtca caccgcaccg ggcagaaaaa
     7741 agcgaacctc gctgttggtt gtagtttttc tgtttgccgt attaaagttt gatcgaaaga
     7801 tttaactgtc cgcctaaacc cacttcaata attttttgca aggttgaaat acgaacatct
     7861 ttgatgtcat tctcaactct tgagatatag gctttgtttg tgccgcattt ttctgcaagc
     7921 tcttcctgtg tcataccttt ttccagccgg gcttcataaa tcaatgctcc cagtttgaac
     7981 tcctgataac ctttttccag attctctctt ttagcacttc ctcttttgcc ataatgtttt
     8041 tctttgaact ggtcgagagt caatgtttta ttttttttca gcataatatt cctcctttat
     8101 ttttaatgct ttctccattt cttgtctcgg tgtcttttga gatttctttt gaaagccact
     8161 tgttactaca atcagtttac ctttatcaaa gaagcaaaat attcttacaa tgtcgcttcc
     8221 ttgctgtacc cggatctcat aaagtccgtc tgtgccttcc aaatgcttaa gatatgtttc
     8281 cggcacttgt ggaaggtctt caatcaattc aaatgtccaa atgattttat ccttaacctt
     8341 ctgacgctgc tttacaaaga actcttcaaa atagttctta taaaaaatga tggttctgtt
     8401 tttgtcattt tcaggcacac tgcaaatata caaaagttgt ccaaaagtac aacattgttt
     8461 tcaacccatg ctggggttcc gaagaagtaa catacaaaac acaacaatgg ccatttatag
     8521 tgttgggaag accaacaaag gcaaaaaata gtgattcact gcaggagcag aaggatatgg
     8581 gaggagatgg ctggcaacag actacttatg gccgtgtaag ccgggatgtg tatggtttta
     8641 agctcgggta tttcagtaat gattatacac ctataggcgg tgcaacggca ggcgcattcg
     8701 gcaataaaac atattcggcc ccgggcagcc ttgatcaaac aggtaatgaa ttatttaatg
     8761 gaaatatttc atttactaca cttgcgttat ccaagctggt gggggcagcc acaaccggat
     8821 acagctatgg gtatgaccaa ttgaaccgcc tggtggaaat gagacagcat agcaccggta
     8881 ccacaagcgg gtggagtaac agcagcatag taactgctta ccgggagagc atcagctatg
     8941 atgccaatgg caatattttg aagtacctgc gaaaggggca tagtgctacc ccgcatatgg
     9001 acagcctgac ataccagtat aattacagca gcggaaagct tattaataac cggctgaatc
     9061 atgtaagcga ctcgatacct gcgggaaatt acagtgagga tatagatgac caggatgccg
     9121 gtaactacca gtatgattat ataggcaatc tgagggcaga tggcgcagaa aagattgaca
     9181 ctatacgctg gacggtgtat ggaaaaataa accggattgt gaaaagtgag gacaatgtga
     9241 gcattgatta cagttatgat gctgccggaa acaggacccg gaagcgggta aacaacaatg
     9301 atacgatcag cattacctgg tatgtgcggg atgcgcaggg aaatgtgatg gcggtgtaca
     9361 gcaataaaga gggaacggag gatatcgtct ggaaggagca gcacttgtat ggcagcagtc
     9421 gcctcggtat gtggcgggca gatacaactg tgccggcatt accgccggtg gtagatgagg
     9481 acccggtgta tgacagtgtg atgctgggta gccgtacata cgagcttacc aatcatttag
     9541 gcaacgtact ggcaaccatc tctgacaaaa aaataggaaa tgacagcagt gacgtggtaa
     9601 attattatat tgcagaagtt ctttcacaga cggattacta tcccggcggg atgctgatgc
     9661 cagacaggaa atacagtgct ggaactggct accggtatgg ctttaatgga aaagagaaag
     9721 atactgaagg cccggtacag tatgattatg ggtttaggat ttatgatccg aggttggtta
     9781 ggttcaaatc tgttgaccca ctaacaagaa gttatcccga actaacaccg tatcagtttg
     9841 caagtaacag accgattgaa gctatagatt tagatggatt ggaggcaaaa gacttataca
     9901 tggagttaga gccagtaaga caacaacgac gaatggaaca atctttcgga attaagcatg
     9961 atgaaactcc tgctgcaaag gccgttggaa tttttgtaca ggtttcttta ggtgcaaccg
    10021 tgttggctcc gattttggtt gaagcaccgg cgttagccac atcaactttt tctagattat
    10081 tttggtgggc agcggcgaat ccggaaacag cagcaggggt gttgtatact attgttgttg
    10141 cagcttctgg atatgaaggc cctgatttac caggccctgc agacgacttt ggcaaatggg
    10201 gcaggaaagt atttgataaa ttaacagcgg ttggagtaaa aagcgagaag tcactagctc
    10261 aggcggctag aaattcatat aatgctatta ttaacactat agacaatgag attaaggata
    10321 tagcgacaat agagcaaaaa gctcgcaaag cttttgatgt aagaaacaag gcaaaggact
    10381 ttgcgagaga actatctgga ccagctaata gagcgaaagc tgaagcgcaa agtctggaga
    10441 aacatggtaa tatcaatggc ccttcatttg atgacttgta taagaagaat tatgacgatg
    10501 caatgaaaag cggattgaaa gaggaggaag ctaaaaacaa agcataccaa ggtattatag
    10561 aatcatctaa acggcctgac caagaaacaa ataaaaaata tggagcaaac tgacaatatg
    10621 caacttgtta aaaaaaataa ccattatgag ttagttgtat tggaatctgc tgtttttgaa
    10681 gactatgaaa atattctgaa ttaccttatg aaagaaatga acttaaaaga tgtagaagaa
    10741 atagatgatt ttgatagccg ttatcaattg tttaattata atgagcaaat aatagtactt
    10801 tactatagta gttttttagg cataagtatt tactttgatg ataagactat tgatagcggt
    10861 gttcaactaa aagtgttaaa agaattttgg gaagtattaa aaaaaagcta atagatttta
    10921 tcgtactata ggttatacaa aatctgtcaa aaaaaaacac aagaagccgt tgagtaatca
    10981 acggtttcta agccatttat cttttacatt ttaattggat tccctttacc aaaatcccct
    11041 cgtaacgggt cagggtctta tagcatggca attcatcaaa gagtatttct gttacattgt
    11101 taattgcctg ctccattggg gtgttgtcaa agtccttata tgacgacatc catatgtcat
    11161 gcatcgtccg cagaatcctt tggttaattc tctccggatt atttataact tcctgcttta
    11221 gcaaagaaag tatctttttt gtacttatca ttaaatctga atcactgctg aagtactttg
    11281 atgtataaga aatagcatct gcctctttca atacattaac tatcattttt tccgcccagt
    11341 gtctcccgcc gcccctcccg taaccgttcc tcccatgtct gccctagtga gggtaagtgt
    11401 tttggcggcg acattgggaa actggaatag ctgtaacaat ttttcagtaa cttccattta
    11461 gttctttgag tgctcagtct atcccggcgg gatgctgatg ccggggagga gttacactat
    11521 aggaaatggc tatcgttatt ccattaatgg gcaagaaaat actccagaga ttgcaccgaa
    11581 tacaactact gctgagttct ggcagtatga tgcgaggata gtcagaaggt ggaatgttga
    11641 cccaatattg aaggagtatg aaagtccata tgcaacattc gggggtaatc ctattattaa
    11701 tattgaccct gatggtagcg atacaataaa tatcaccagg aaaactacat tcgataaaag
    11761 acaatatagg ccaccttcaa tttcaggtaa tccaaatagt cataatagag tatatgatcc
    11821 tgggaacgat ggaatcacaa gatcaggcag catagaaata atggaggctg caggacaaga
    11881 tatatttagg attacagacc tgcacattca catagatgag aatggaaatg aaagtacggt
    11941 atcctctacg accacaacac tggaacttag taaaatatct gcaggtcgaa tagttgcagg
    12001 ccggaatgta aaaggatatg ctgatgatag gtatgctttg gcagctcatg caccaaggtg
    12061 gcttctagag tactatgaaa agaaaactgg tggagatatt ggaataagat ctgcaatagc
    12121 ctatcaaaaa actgtaccat ttgctcatgg tttaggtaga ataacaaata ttgcttatac
    12181 agtagcgggt gtttatggat tggccagagg ctttttagga cgcaatgccg ctggtattgg
    12241 atttaacaca tttaatgaat ttaaaactgc ttatggcact gctggtattg gtaaagcctg
    12301 gcatcacatt gtagaacaaa atcccgcaaa cattgctaag tttggagctc aacgtataca
    12361 taattcaacg aatcttataa aacttgacca tggagccgga actattcatg caaaggtttc
    12421 tggttattat tcttcaattc aaccgtttac caatggccaa acagttcgga aatggttaag
    12481 tagtcaaagc taccagcagc agtatgattt tggaattaaa acaatgaaac aatttggttg
    12541 gacaccatga aaataaagga taaaaaaacc gctttagctt catttgaaga atcagcagtt
    12601 aaacatgctg acgctacagg acaaggagat tataagacag caaacaaatg ctatgctaac
    12661 atagtgaaag caattaattt tcttaaagag caaggtgagg ttgcagcttt aattgattgc
    12721 ttatgtcatt cttcggctgg tgtacgcaat tgggcagcta cttatcttct acctgtgaag
    12781 gaaaaagaag ctataaaagt attggaagaa atagtaagga aaaaagatat acattctctt
    12841 agtgcagaaa ctacacttag tgaatggcga aagggaaacc tgaagctttg atgttgaaca
    12901 aaataattat gttatagaat atatgtttaa gtatttgctt gctaatcttt ctgacacacc
    12961 taaagaagta aattttaaag atatatctga atttgacaga atggaacaaa gattctttgc
    13021 tattgagcaa tattacgggg atattgttgg gatgaatgtt gattctgttg agttttgccc
    13081 taatcataac ccccctgaat tacaaaatga aatactgccc tggttgtggc ttattcatcc
    13141 agaattgaaa gaccaaatat cagagatagc agatagtcaa ttaaggcaga ttataagaga
    13201 atatcggaat cacaaagaac cttagtaatg cactattatt tgcacttttc tctccagagt
    13261 ctcctcgccc ccgtaaattt ccacccatgt ctgcccgagt gtgggtaagt gttttggcag
    13321 gcgacattgg aaaactggaa tagctgtaac attttttcag taacttccat gtagttcttt
    13381 gattgctcag tctatcccgg cgggatgctg atgccggaca ggcaatacag cgctgcgact
    13441 ggctaccggt atgggtttaa tggaaaagag aaagatactg aaagcccggt gcagtatgat
    13501 tatgggttta ggatttatga cccgaggtta gtaaggttta agtcggttga tccattatca
    13561 aaaaattatc cttcatggag tccataccca tttgcaatga accgaccaat agatggtatt
    13621 gatttagatg gcctagaata tttaaaggcg aatgaatcaa aaattgatat ttatgtttca
    13681 tacctagaat ttaatggtag aaaagttatt tatggaagcg ttcatttgga tatgaataat
    13741 gtttctgcaa atacgcagaa ttatgttaat aattctcctt ggaagaatgg agcaataggt
    13801 agtggaagga gtaaaattgc aacttttgaa catatcgatt ataacgatat actagctaaa
    13861 aaacaagaag cattcaatgt tctgtttaaa caagccgatg caacagatat ggaagcaaaa
    13921 tctttagtgc aacccaatct tgaagttccc agaattccta agaataagtt agaagaaaga
    13981 caaattcaga aatcaaaaca gttcacactt ccatcagggg gcggttcatc tgctaaaggt
    14041 aatgcaattg tagcagcagt tcagcttgtt ggggaagtac ttcagtattg gcatggaaaa
    14101 aaagttgata acgattataa tacagcaaac tcacagttgg cctcagggca atttgccctc
    14161 aacgatttac aatttgctct tgataataat ttaatcccat cccaatactt aggtaatgtt
    14221 tcggaatttg caaattattt acttgatggg aaaaaaccaa ctattacata ttacattaaa
    14281 aacgataaag gcgagaatgt tccgaaggag tctgtaaata acaatttaat aaaggcattt
    14341 aatgagatta agtcaaatat ctcaaaagag agagccaaat taagtgaacc taaacaatgt
    14401 gttccatgcg aggaataatc ctattaaaaa atagaggcat ttttattatt caagtttttt
    14461 ttgtaatatt tttatttgca tgttcacaaa aagcccttaa ggattctcgt tttgaaaatt
    14521 taattattga tgctcagaaa aatatagaaa atggaaatta ttaaaaagat ttgattttat
    14581 ttaaagatga aataaaattg aactcatcc
//
LOCUS       DAORTI010000088        14359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4069_length_14359_cov_5.829209, whole genome shotgun sequence.
ACCESSION   DAORTI010000088 DAORTI010000000
VERSION     DAORTI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_14359_cov_5.829209"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLO70_11330"
     CDS             <1..640
                     /locus_tag="PLO70_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75103.1"
                     /translation="QTESIATVSPVRLFTSNQEITDTQIIRKFVNDNTFTSFAPFIIL
                     NSNEQVFNYVKLRISFISSDSAIVERYPLAYPQFTGPIENHMASYRLTTTGNLYIREK
                     DTIQYSHSLNIIPRCQELLNMSLFDYNPDDNCMPSGMNWISCKFLNSFTIKKVNQNLS
                     LPLIVTIIKSGNQCLLNTFHDEWDFFNPNILTQLQITDTIAIQTKELFLQKL"
     gene            648..977
                     /locus_tag="PLO70_11335"
     CDS             648..977
                     /locus_tag="PLO70_11335"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HQZ75104.1"
                     /translation="MKLLSFPFQTLNWSSIPKEEHNGETGVAWWQVQLVNDIRVRLVE
                     YSPGYKADHWCSKGHIIFCFEGEMDTELEDGRVTKLTKGMCYFVGDNNEAHRTSTASG
                     CKLFIVD"
     gene            1028..1552
                     /locus_tag="PLO70_11340"
     CDS             1028..1552
                     /locus_tag="PLO70_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75105.1"
                     /translation="MQKLIQSIFAFLLINGNSQAQNPFELGAEYIRSSGKGYSTTKVA
                     ARGESFNNKNSFSAGITYQLASAKAYSVSTGFGMYVGYRHAFGTNTNGNNPFAGARIL
                     FSLEDFEGKTRQKSLLVTPMAEAGYHFIFAKRFYGAPSVGFGYTIEFSKGYNSLDEDV
                     GKRFIPSISAGYRF"
     gene            complement(1554..2273)
                     /locus_tag="PLO70_11345"
     CDS             complement(1554..2273)
                     /locus_tag="PLO70_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQZ75106.1"
                     /translation="MHPLVRILFTGMLVSFIGSLPLGTLNIAAMQISITDGVTAAMLF
                     SVGSLIAEIIYVRISLVAMDWVRKQEKILKALDWVTFLIVAALAAFSFYAAMQPTVEK
                     NIVLSSPLPKIVLGFVMSAVNPVQIPFWFGWSTVLFTKKVLLPRSDHYNSYIVGIGLG
                     TFLGNCIFIFGGLLIAQKINNNQHVLNWIIGGIFALTALLQLWKILRKKDTMHKLEHP
                     EEVTQHIEEQIDKLGIEPDEK"
     gene            complement(2300..3340)
                     /locus_tag="PLO70_11350"
     CDS             complement(2300..3340)
                     /locus_tag="PLO70_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75107.1"
                     /translation="MSSKKFFQKIVNWELWNFYVLYLPISPVWLWYCLRSRSFWYFSS
                     SNPTITFGGFEGEGKKEMYDQLPTELVPRTIYIMHDLPYENVERIVNEAGFSFPFIVK
                     PDVGMKGILFRKIDNADQLRKYHEKVPVEYIVQDLVDLPVEVSVFYYRHPAEKKGIVS
                     GFIHKELLQVKGDGQSTLRQLVEQHPRAKFRMEEMGHRHGHRFDRVIPEGEIFYLSYA
                     GNHNRGAQFTNLHNEINEVMHQVFDELSHKTRFYYGRYDIKTTSIEDLQQGKNFLILE
                     FNGCGAEPNHIYDCGMSLWKAYGVILEHWKALYRISRHNHKNGTPYWSFKKGWNFLKE
                     SGRHFKMLEKYD"
     gene            3423..3926
                     /locus_tag="PLO70_11355"
     CDS             3423..3926
                     /locus_tag="PLO70_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75108.1"
                     /translation="MRIFLIWLSIFSCFFTAAIPAQAATAPLSNNKIIPVEAIYKKIA
                     SMKMKDFRKLAGRKLTLKEKVSFIILKHKLKQQAKDSEKQGQTAFTFGLIAVGLLLLG
                     LFVPYVILGSLVAAILAIVTGSMAKKRDGDNRKAKAGQLLGWITLGLIAFLFILAAVV
                     LASWSWG"
     gene            3982..4761
                     /locus_tag="PLO70_11360"
     CDS             3982..4761
                     /locus_tag="PLO70_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQZ75109.1"
                     /translation="MNIKTAADFFANGQHPEILFWVGCAGSFDQRAQKITKAFVTILD
                     KVGINYAILGKEEMCTGDPVRRAGNEFMFQMMAYQNIQILNNYGIKKIVTACPHCFNT
                     LKNEYPVLGGTYEVIHHTTFLQSLIDEGKIKMKEGGIFKGKKITYHDSCYLGRSNNIY
                     EAPRKVLEALDAELVEMKRCRSNGLCCGAGGAQMFKEEEKGTSRVNIDRSNEAIATGA
                     SVIAAACPFCNTMMTDGVKLADKEETVQVLDVAELIAQSME"
     gene            complement(4805..5209)
                     /locus_tag="PLO70_11365"
     CDS             complement(4805..5209)
                     /locus_tag="PLO70_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75110.1"
                     /translation="MEQTNKNDKLLAHWTYARDEWNKFLKWSFFKKGFIFYLFHLLNP
                     FKQKGSAEVKISSRCISINENRSEVNFPDQYFADVDIYEAGNLNILEILYKNGHHRRK
                     ISIPIPKGKLKEAIDVQMRLTRQYTKAIVADR"
     gene            5241..5765
                     /locus_tag="PLO70_11370"
     CDS             5241..5765
                     /locus_tag="PLO70_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75111.1"
                     /translation="MNLDYKYLLEGNFHPDSKVWVYQSNRLFSLGEALEIEEILKDFT
                     GKWQSHGAQVKGAAYLFFGQFIILMADETATGVSGCSTDSSVRLIKEIEQRFGVNMFD
                     RTTLAFVVKDKVQLLPLSQLQYAAENGFIHNDTLYFNNLVQTKAELENKWIIPVKDSW
                     LANRITFSIPVANL"
     gene            5768..5840
                     /locus_tag="PLO70_11375"
     tRNA            5768..5840
                     /locus_tag="PLO70_11375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5801..5803,aa:Gly,seq:ccc)
     gene            complement(5849..8773)
                     /locus_tag="PLO70_11380"
     CDS             complement(5849..8773)
                     /locus_tag="PLO70_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75112.1"
                     /translation="MRIFFISLAVIFLFIFFNNQASAQCAENFDGVTAPALPAGWSAV
                     TLTDCTGSNPWVTSTTTPSSAPNAAFVIAPACVSDEVLVSRQYLITSSTAQLTFRRRH
                     GLEAAMDGLVLEISINGGSFEDISYTGSFGAGGYNTILNSSGNPLAGREAWSGSTSNA
                     YVTTTVNLPASVIGKVVVFRWRRGTNASVSGTGVYIDNISLTGCSISVCAENFDGITA
                     PALPSGWIAFTARDCTGSNPWATVNNWSHTVPNAAYVSAADCVSDEYLYSKVFQIVSA
                     TAQINFRRSFSLESNFDGLVLEISIGGAPFVDIQAAGGLFITGGYNGVISNCCGNPLG
                     GRSAWTGNVLTWTLTTINLPASANGKTVILRWRRGTDSSTPSAGAHIDGLSITGSLCS
                     PVCATRITLTPAGGNLCSTNPIVLTASVAGASYEWYRNDVKIDDVSGNTYSAISSGIY
                     YAKAVIGGCLVTSNVSVLDAGTETPALNGGGVYCPGSSVSLTVPESKSTQDYTWKKNG
                     VVVYGPVTGNDGPLSYNFNMDAGMVGDYSVETYKPGCVGVVSNIAYVRLSEQVTGLTT
                     EQVCPNQAIIKWNKVIPNPFPERYEYHLSESSTPPSSGSQVFDSVITLPVNPSTLYYF
                     YVRSYCEGSTGTWSSISFTTPVQSTLTLSPANATICNGSPVTLTASGGGSSYALYWNG
                     SIQYGSNSPTFTISTSGTYTVTSEVNGCTLTSNSSVINTATLFATTLSGSGVYCTGEL
                     VDLRTTTQPSQQYSWYSSGSLVYGPVGGGNGGNQSYVLTMSAGAEGSYYVKTTSSGCE
                     TQSNSVIINEAAINNLTATKICSNQAHFSWDYILTEFQYVVDQNSSPPVNPSNPIFTN
                     NTDITVNLLLPATTYYIHVRARANNSANTDYCSNWTTLSFTTEPENAAEWTGELSTNW
                     FDGGNWKCGTVPGPGFEVVVNAGKPNYPVITSNTTIKKLTVNSGASVNVSTGVTLTIT
                     SQ"
     gene            complement(8798..12523)
                     /locus_tag="PLO70_11385"
     CDS             complement(8798..12523)
                     /locus_tag="PLO70_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75113.1"
                     /translation="MKRIFISCTVIFLFFISVINKASAQCTENFDAVTAPALPAGWSA
                     VTLTDCGSSNPWVTSTTTPDNGINTAFVNAPGCVSDEVLVSKYFSITSAAAQLTFRRK
                     HELELNYDGLVLEISIDGGSFVDILAAGGGFTAGGYPATSLWFGSNPIGGRRAWTGST
                     SGAFVTTTVTLPASANGKIIALRWRRGTDHIVSATGVFIDNITITGCSTPVLSCTQNF
                     DGVTAPALPAAWTAATALDCVNSNPWVTASSTPDSPPNSVFVNDPDCVSDEYLYSRLF
                     QIVSASAQITFRRNHDLELNYDGMVLEISIGGMPFTDILLAGGSFVVGGYNAAISNCC
                     GNPLSNRSAWTGSSGGWVTTTINLPVSANNKSVVLRWRRGTDNSVTGTGAYIDGLSIT
                     GAVCFTPCANTIILSPNGGSICSSNQIVLTASGANGNYEWYKNDVKINDAFGDTYTAI
                     TGGIYYVRSMISGCLVTSNVAVLEPGTIVPGITGTGVYCLGSSVSIGMAVSQTDQEYT
                     WKKNGIAVNGPVSGTGGSMSFNFTMDAGRAGKYVVETTKPGCIAAVSDTVYIGSPDIT
                     GLSTVTVCSDQAIIKWNRVIPQFISQTYEYEITQSSSPTGNSNYTADSVRSVTSLIPS
                     TQYYFHIRSTCDLSGFGNWTSISFTTSPSTSLTLSPTTGSFCNNPSPITITASGGGAS
                     YSWFKDGSFIFGESGSSINATSGGVYTATSTIGGCTLSSNEAILESGSVPPNLGGTGI
                     YCLGESVNVGIPVTELGQDYTWRQNGLAVYGPIGGNGGNQSLQFNMVENRAGVYYVES
                     TRPGCGVVYSGTVYVGFAKINNLVTTGICANQVTFKWSRVAPVSISQTYEYEVTQSSL
                     PTGNGTLTSDSSITVTSLNPSAIYYIHVRAACNFGSSFGDWTTISFTTSSSNFSLSPT
                     SGNVCNNTLLLTASGNATIYEWYRDNNLIDGATGATYNASTAGTYRAETIFNGCRNVS
                     NNAVITETTIVDPIVDLGTIGVTSRVYCAGALVDIRITNSELSQQYSWYRNGSLYSGP
                     IGGNGGNQSLPIVMSSSQDATWTVRTSKAGCGDETSNAVFVDEARVTGLNTTAICSNQ
                     VSFEWNSIVTGENYQYVVNQSITPPSNPVNPGTTSSTSATVSSLLPSTAYYIHMRAAS
                     GPDYTSFCSTWTTISFSTLASNAILLWTGAFDNSWTNPSNWACGQVPGPTSEVVINGG
                     LPNYPFITSNATIKKLSVNPGASVTVEPGVTLTITSQ"
     gene            complement(12558..>14359)
                     /locus_tag="PLO70_11390"
     CDS             complement(12558..>14359)
                     /locus_tag="PLO70_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQZ75114.1"
                     /translation="FFLRAREIWKKRLGESHPFYTENSLSLARVYWGKNNVEKANELY
                     VKAFEAQHLQSASLFSFTSEEEKQLYLKNITGAEDEYQSFYFDKMNRDNRGQPYLISL
                     QKRNQILSSSQQLRQLIYTSNDTTLTRTYDTWISYKKQIAAIYSKGDMAKNEHLAGIK
                     EKADLLEKDLTRRSSAFGKAQQKSISWEDIQQQLKPSDAAIEFIEFNYFNGHRTTDSI
                     FYAALVLRKGMKEPEYVTLFEKRQVDSLLRKTASGSQSINTFYTRGLKVKTESSLSRL
                     AFNTIWKPLEEKLKGVNTIYFAPAGLLHRIAFAALPVDSATLLSDKYQLFQVSTTAAV
                     TAQTADYVKPDDKIYLFGGVQYAQVAPKTKPAVNTGPLAKRSGFDYLPGTAMEVNQVA
                     LAGRSKKYSPVVIKGWDAREDSVKQLTGKGSPRVLHIATHGFFFDDPQKTTIENQADS
                     SVGGKAFQQSENPLFRSGLLFAGANDTWKGKPPGEMEEDGILTAYEVANLYLPNTKLV
                     ALSACETALGDIKGSEGVYGLQRAFKMAGAENLLMSLWKVPDAETAEFMHLFYKNVFD
                     QKAIDKSFYEAQAVMRNKYRHSPYKWAAWILIR"
BASE COUNT         4264 a         3082 c         2792 g         4221 t
ORIGIN      
        1 tcaaactgaa agcattgcaa ctgtttcgcc tgtaaggctc tttacaagca atcaagaaat
       61 aactgacact cagattatac gaaaattcgt caacgataac acatttacca gttttgctcc
      121 atttattatt ctaaattcaa atgaacaagt attcaactat gtaaaactta gaatctcttt
      181 cattagttct gactctgcaa tagttgaaag atatccacta gcctatcctc aatttacagg
      241 gccaattgag aatcatatgg caagttatcg tcttacaacc acaggaaatt tatatatacg
      301 agaaaaagat accatccaat attctcattc attgaatatt attccaagat gccaagaatt
      361 actaaatatg agcttatttg attacaatcc tgatgataat tgtatgccct caggaatgaa
      421 ttggatatct tgcaaatttt taaactcctt tactattaaa aaagtaaatc aaaatttatc
      481 attgccatta attgtaacaa taattaaatc aggaaaccag tgtctactta ataccttcca
      541 cgatgaatgg gattttttca atccaaatat tctgacacaa ctgcaaataa cagatactat
      601 agcaatacaa acaaaagaat tgtttcttca aaaattatag tatttatatg aagcttcttt
      661 ctttcccttt tcaaacactc aactggtctt ccattcccaa agaagagcac aacggtgaaa
      721 caggagtagc ctggtggcag gtacaattgg taaatgatat cagggttaga ttagtggaat
      781 attctcccgg gtacaaagcc gatcactggt gcagtaaagg tcatattatt ttttgcttcg
      841 aaggagaaat ggataccgaa ctggaggatg gccgggtgac gaaacttaca aaaggtatgt
      901 gctattttgt gggtgataat aatgaagcac accgcacttc cacggcaagc ggatgtaaat
      961 tattcattgt tgattaactg gtcattgata gtataatata ttctcctgtc aaacatttag
     1021 ataagcaatg caaaaactta ttcaatcaat cttcgccttc ttgctgatca atgggaatag
     1081 ccaggcacaa aatccttttg aactgggagc agaatatatc cggtcatccg gaaagggata
     1141 cagcactacc aaagtagcag cgagaggtga atcatttaac aataagaaca gtttcagtgc
     1201 aggcattact taccagcttg cttccgcaaa ggcttactcg gtttctaccg gttttggaat
     1261 gtatgtgggc tatcgtcatg cctttggtac caatacaaat ggaaacaatc ctttcgccgg
     1321 tgccaggata ttattctcac tggaggattt tgaaggaaaa acaagacaaa aaagtttatt
     1381 ggtaactccc atggctgaag cgggatacca cttcattttt gcaaaacgat tttacggagc
     1441 cccttccgtc ggttttggtt ataccattga attcagtaaa ggatataatt cgctggatga
     1501 agatgtaggc aagcggttta ttccatccat ttctgccggt taccggtttt gaattatttt
     1561 tcatccggtt cgatacccag tttatctatc tgttcctcta tatgctgtgt tacttcttcc
     1621 gggtgttcca gtttgtgcat tgtatctttc ttgcgtaata ttttccatag ctgtaacagc
     1681 gctgtcagag caaatatgcc accaataatc cagttcaaca catgctgatt attattgatc
     1741 ttttgtgcaa taagcaaacc gccgaaaatg aaaatacaat tacctaaaaa tgtacccagt
     1801 cctatcccga ctatatatga attgtaatga tcagatcttg gtaacaatac tttcttggtg
     1861 aataatactg tgctccagcc aaaccagaaa ggtatctgaa cagggttcac cgcactcatc
     1921 acaaagccca atacgatctt tggcaatggg ctgcttaata ctatattttt ttctactgtt
     1981 ggttgcattg ccgcataaaa gctaaaagca gctaatgcag caacaataag gaaagttacc
     2041 caatcaaggg ctttcaggat cttttcttgt tttcttaccc agtccatagc caccagtgag
     2101 atgcggacat aaattatttc ggcgatcagg gaacctactg aaaaaagcat agcagctgta
     2161 accccatccg tgatagatat ctgcattgca gctatattca gcgtacctaa cggtaatgag
     2221 ccaataaaac tgacaagcat gcctgtaaat aaaattctaa ctaaggggtg cataaactaa
     2281 aaatacgaag agaagagcat taatcatatt tctccagcat cttaaaatga cggccggatt
     2341 ctttcaggaa attccaccct tttttaaaag accagtaagg ggtaccattt ttatgattgt
     2401 gacggctgat cctgtataat gctttccaat gttctagtat aacaccataa gctttccaga
     2461 gactcatacc acaatcgtag atatgatttg gctctgcacc acaaccattg aattcaagaa
     2521 taagaaagtt cttcccttgt tgcaggtctt caatagaggt tgttttaata tcataccggc
     2581 cataataaaa acgggtctta tggctcagtt catcaaatac ctggtgcatc acttcattga
     2641 tctcgttatg aaggttggtg aattgtgcac ccctgttatg attaccggca taagaaagat
     2701 aaaatatttc accttcggga atcaccctgt caaaacgatg cccatgccgg tgccccattt
     2761 cttccatccg gaatttggcc cggggatgtt gttcaaccaa ttgcctgagt gttgattgcc
     2821 cgtcaccttt tacctgcaat aattctttat gaataaatcc gctcactatt cctttttttt
     2881 cagcggggtg gcgataataa aaaacactca cttccaccgg cagatccacc aggtcctgaa
     2941 caatatattc taccggtact ttttcatggt atttccggag ctgatcagca ttgtcaattt
     3001 tacggaataa gattcctttc atccccacat ccggttttac aataaaagga aatgaaaacc
     3061 ctgcttcgtt cacaattctt tctacatttt cataaggcaa gtcatgcata atataaatag
     3121 tgcggggaac cagttctgtg ggtagctggt catacatttc ttttttccct tctccttcaa
     3181 aaccaccgaa ggtgatagta ggatttgacg aactgaaata ccagaatgac ctgctgcgca
     3241 ggcaatacca tagccagact ggacttatag gcaggtagag aacataaaaa ttccagagtt
     3301 cccagttaac tattttttga aaaaatttct tactactcat tgaaagctgc aaaaataact
     3361 attgagtgcc ttatttggca atatattcaa tatgctttac atttattctc aaactaactg
     3421 tcatgagaat tttccttatc tggctcagca ttttcagttg cttttttact gccgccatac
     3481 ctgcacaggc cgccactgcg cctctttcca ataataaaat aattccggtt gaagctattt
     3541 acaaaaagat cgcttccatg aaaatgaaag acttccggaa attggccggg agaaaactta
     3601 ccttaaaaga gaaagtatcc tttattatac tcaaacataa actgaagcaa caggcaaaag
     3661 acagcgaaaa acagggacag actgctttta cttttgggtt gatcgcagtt ggcctgctac
     3721 ttctcggtct ttttgttcct tatgttatac tgggttccct tgttgcagcc atccttgcga
     3781 tcgtaacagg gtcaatggct aaaaaaaggg atggtgacaa caggaaagca aaagccggtc
     3841 aattattggg atggatcact ctcggcctga tagcattttt attcattctt gcagctgttg
     3901 tgctggcctc ctggtcatgg ggttaatgcc tgagctttat ttttattccg tattattgct
     3961 gcgaaaaatt gatatgaatt catgaatatt aaaacagcag ctgatttttt tgcaaacggt
     4021 caacatccgg aaattttatt ttgggtagga tgtgccggta gttttgacca gcgggcacag
     4081 aagatcacta aagcctttgt caccattctt gataaagttg gtataaacta tgccattctt
     4141 ggtaaagaag aaatgtgtac cggcgatcct gtacgaaggg ccgggaatga attcatgttc
     4201 cagatgatgg cgtaccagaa tatccagatc ctgaataatt atggtatcaa aaaaatagtg
     4261 actgcctgtc cccattgctt caatacactg aagaacgaat accctgtatt aggcggaact
     4321 tacgaagtca ttcatcatac cacctttttg caaagtttga ttgatgaggg aaaaataaaa
     4381 atgaaggaag gtggcatttt caaaggcaaa aaaatcacct accacgatag ttgctatctc
     4441 ggccgctcca ataatattta cgaagcacca agaaaagtac tggaagccct ggatgcagaa
     4501 ctggtggaaa tgaaacgctg ccgcagcaat ggattatgtt gtggcgctgg aggtgcacag
     4561 atgttcaaag aagaagaaaa aggaaccagc cgggttaaca ttgatcgtag taatgaagcc
     4621 attgccaccg gtgccagtgt gattgctgct gcctgccctt tctgtaatac aatgatgaca
     4681 gatggtgtaa agctggctga taaagaagag accgtacagg tacttgatgt agctgagctg
     4741 attgcacaat caatggaata aacgattgta tcattcttac ctggaattat tctaaatcgg
     4801 gtgatcaccg gtcagcaaca atagctttgg tgtactgccg ggtgagtctc atttgcacat
     4861 cgatagcctc ctttaatttc cctttgggaa taggaatact tattttacgc cgatggtgcc
     4921 catttttata cagtatctcg agtatattta aattgcctgc ttcataaata tcgacatcgg
     4981 caaaatactg atccgggaag tttacttcgc tccggttttc atttatagag atacaacggg
     5041 atgaaatttt tacttcagca ctaccctttt gtttaaacgg gtttagcaga tggaacaggt
     5101 aaaaaataaa tccttttttg aaaaaactcc atttcaaaaa cttgttccat tcgtcccggg
     5161 cataggtcca gtgcgccagc agtttgtcat ttttatttgt ttgttccatc aggattataa
     5221 attggttgta atttagcagc atgaatcttg actacaaata cctgctggag ggtaattttc
     5281 accctgactc aaaagtgtgg gtgtatcaaa gtaatcgtct gttttctctt ggtgaagcgc
     5341 tggagatcga agaaattctg aaagacttta ccgggaaatg gcagagccac ggtgcccagg
     5401 ttaaaggggc tgcttatctc ttttttgggc agttcattat cctgatggcc gatgaaacag
     5461 ctaccggtgt cagcgggtgc agcaccgaca gctctgtacg gttaataaaa gagatcgaac
     5521 aacggtttgg tgttaatatg tttgaccgta caacactggc ttttgtagtg aaagacaaag
     5581 tgcagttatt gcctctttca caattacaat atgctgcgga gaatggattc atccataatg
     5641 atactttata cttcaacaac ctggtacaga caaaagctga actggaaaat aaatggatca
     5701 tcccggtaaa ggatagctgg ctggccaaca ggattacatt ttcaattcct gttgccaacc
     5761 tataaaagcg gaagtagctc acctggtaga gcgacagctt cccaagctgt aggtagcggg
     5821 ttcgagtccc gtcttccgct cttttctttt actggcttgt tatagtcagc gttactccgg
     5881 ttgaaacgtt tactgatgcc ccggaattaa ctgtaagttt ctttattgta gtattagatg
     5941 tgataaccgg ataattgggt ttgccggcat ttacaacaac ttcgaaacct ggccccggaa
     6001 cagttccgca tttccaattc ccgccatcaa accagtttgt gcttaattca ccagtccatt
     6061 ctgcagcatt ttctggttca gtagtaaaag acaatgtcgt ccagttagag caatagtcgg
     6121 tatttgcaga attattggcc cttgccctta catggatata atatgttgtt gcaggtaata
     6181 gcaaattaac agtaatatca gtattattcg taaatatcgg atttgaggga ttaacaggag
     6241 gtgaactgtt ttgatcaacc acatattgaa attccgttag aatataatcc cagctaaaat
     6301 gggcctggtt cgaacaaatc tttgttgcag taagattgtt tatagctgct tcgttaatta
     6361 taacagagtt tgattgtgtc tcacaacctg atgatgtggt tttgacataa taactgcctt
     6421 ctgcgccggc agacatcgtt agcacataac tttggttacc tccatttcct ccaccaacag
     6481 ggccatacac aagagaaccg gagctatacc aggagtactg ttgtgatggt tgtgtagtcg
     6541 ttctcaaatc aaccaactcg ccggtacaat aaacccctga tccggaaaga gttgttgcaa
     6601 acaatgtggc agtattaatt actgaagaat ttgaagttaa agtacaacca tttacctcag
     6661 atgtcactgt ataagtaccg gatgtactaa tagtgaaagt ggggctattt gaaccatact
     6721 gaatgctgcc attccagtac aaagcataac tgcttccacc accagacgct gttaaggtta
     6781 ccgggctacc attacaaatt gttgcatttg caggactcag agttagagta ctttgtacag
     6841 gagttgtaaa tgaaatactg ctccatgttc ctgttgaacc ttcacaataa gaacgtacat
     6901 aaaaataata cagtgttgaa ggattgacag gtagagttat aacactatca aaaacctgtg
     6961 aacctgagga aggtggggta cttgactctg aaagatgata ttcatatcgt tcaggaaaag
     7021 gattcggaat caccttattc catttaataa ttgcctgatt tgggcaaacc tgttctgtag
     7081 taagtcccgt tacctgttcc gataatctca cataagcaat atttgaaacg actccaacac
     7141 atcccggttt atatgtttct acagaataat cacccaccat tcctgcatcc atattaaaat
     7201 tatagcttaa tggcccgtca ttgccagtta cagggccgta aactactact ccattttttt
     7261 tccaggtata atcctgtgtg cttttcgatt ccggcacagt aagacttaca gaagaacccg
     7321 gacaataaac tccgccaccg ttaagcgccg gagtttcggt gccagcgtct aaaactgata
     7381 cattagatgt aactaaacat cccccgataa ctgcttttgc atagtaaata ccactgctta
     7441 ttgctgaata tgtattcccg gatacatcat caatcttaac atcattacgg taccattcat
     7501 atgatgcccc agctacagaa gccgtcaaaa caattggatt agtgctgcat aaatttccac
     7561 ccgcaggtgt caatgtgata cgggtggcgc atacggggct acacaatgaa cctgtaattg
     7621 aaagtccatc aatatgtgca cctgcgctgg gggtggaact atcagttccc cttctccatc
     7681 tcaggataac cgtttttcca ttagctgaag cgggaagatt aattgttgtt agagtccatg
     7741 taagtacatt cccggtccag gcagaacggc cccctaaggg attaccacaa caattactta
     7801 taacgccatt atatcctcca gtaataaaga ggcctcctgc tgcctgaata tcaacaaaag
     7861 gagccccgcc aatagatatc tccaatacca aaccatcaaa attagattca agactaaaac
     7921 ttcttctaaa atttatttgt gctgttgctg aaactatctg gaatactttt gaatacaggt
     7981 attcatccga aacacaatcg gcagcagata catatgcagc gtttggaact gtatgagacc
     8041 agttattaac tgtagcccat ggatttgaac cagtacaatc ccttgcagtg aatgctatcc
     8101 atccggaagg caaagccggt gcagtgatcc cgtcaaagtt ttcagcacat accgaaattg
     8161 aacaaccggt aagactgata ttatcaatat atactcctgt tcctgataca cttgcattag
     8221 taccccttcg ccatctaaaa acaacaactt tcccaattac agatgccggc agatttactg
     8281 ttgttgttac ataagcattt gaagtggatc cgctccatgc ctctcttcct gcaagagggt
     8341 tgccgcttga gttgagtatt gtattataac caccggctcc aaagctcccc gtataagaaa
     8401 tatcttcaaa actcccgcca ttaatagata tctccagtac taacccatcc atagcagctt
     8461 ctaagccatg ccttctccta aaagttaatt gggcagttga gctggttatt aaatactgtc
     8521 ttgaaactaa tacttcatca gaaacacagg caggtgcaat aacaaatgca gcattaggtg
     8581 cactgctggg agttgttgtc gatgtaaccc atggatttga acctgtgcaa tcagtcaacg
     8641 ttacagcact ccagccggct ggtaaagcgg gagctgttac tccatcaaaa ttttcggcgc
     8701 attgagctga tgcctgatta ttaaaaaaaa taaataagaa gattactgca agtgaaataa
     8761 aaaaaattct catctgttga tggttttgtt atcagtttta ttggcttgtt atagttaatg
     8821 ttactcccgg ctctacggta acagaagctc ccggattaac ggaaagtttt tttatagtgg
     8881 cattcgatgt tataaaagga tagtttggca accctccgtt gataaccact tctgatgttg
     8941 gccccggtac ctgtccgcat gcccagttag aggggttcgt ccacgaatta tcaaaagcac
     9001 ctgtccacag caatattgca ttactggcca gggtggaaaa tgaaatagta gtccacgtag
     9061 aacaaaatga agtgtagtca ggtccgctgg cagcccgcat atggatataa taagcggttg
     9121 aaggcaataa ggatgaaaca gtggccgagg ttgatgaggt ggtacctgga ttgacaggat
     9181 ttgaaggtgg agttatgctt tgattaacta catactgata attttctccc gttacaatgc
     9241 tgttccattc aaaactaacc tggtttgaac agattgccgt tgtattcaga cctgtaaccc
     9301 tcgcctcatc aacaaaaact gcattagatg tttcatcacc acatcctgct tttgaagttc
     9361 ttacagtcca ggtggcgtcc tgcgatgagg acataacaat tggcaggctt tggttaccac
     9421 catttccacc aattggtccg gaataaagtg aaccattgcg ataccaggaa tattgctgcg
     9481 aaagttcaga attagttatt ctgatatcaa ccagagcacc agcacaatat actctactgg
     9541 taactccaat agtacctaaa tctacaattg gatcaacaat tgtagtttca gttattactg
     9601 cattattaga aacattcctg catccattaa agattgtttc tgctctataa gtaccggctg
     9661 tgcttgcatt atatgttgcg cctgttgcac cgtctattaa gttattgtcc ctgtaccatt
     9721 cataaatagt tgcattaccg gatgctgtta acagcaaagt attattacat acatttccgg
     9781 aagttgggct taaactaaaa ttgctggaag aagtggtgaa agaaatggtg gtccagtctc
     9841 caaacgaaga accaaaattg caggcagcac gaacatggat ataataaatt gcagaagggt
     9901 tcaatgatgt aaccgtaata ctactgtccg aagtcaaagt cccgttacct gttggcaacg
     9961 atgactgtgt aacctcatac tcataagttt gggaaatact aaccggggcc accctgctcc
    10021 atttaaatgt tacctggttg gcacaaatac ctgttgttac caaattattg attttagcaa
    10081 accctacata cacagtacca gaatatacaa caccacaacc tggtcttgtt gactctacgt
    10141 agtatactcc tgcccgattc tctaccatat taaattggag gctttggttt ccaccattac
    10201 cgccaattgg gccatacaca gcaagaccat tttgccgcca ggtataatcc tgtcctaatt
    10261 ctgttaccgg tattcctaca ttcacggact ctcccaaaca ataaatacct gtaccaccaa
    10321 gattgggtgg aacagaacca gattcaagaa ttgcttcatt tgatgaaagc gtacagcccc
    10381 caatagttga tgtggcagta tacaccccac cggaagtagc attaattgaa gaaccgcttt
    10441 ctccaaaaat aaatgaacca tctttaaacc atgaatagct tgcaccacca ccggaagccg
    10501 tgattgtaat tggagaagga ttgttacaaa aactgcctgt agtgggactt aatgttaaac
    10561 tagtagaggg agaagtagta aatgaaatac tggtccagtt tccaaatcct gaaagatcac
    10621 aagtagaacg aatatgaaaa taatactgtg ttgagggaat caatgatgta accgacctga
    10681 cactatctgc agtgtaattg ctattacctg ttggcgaaga agactgagta atctcatact
    10741 cataagtttg tgaaataaat tgcgggatca cccggttcca tttaattatt gcctggtctg
    10801 agcaaaccgt aacagtagag aggccggtta tatccggtga tcctatatag acagtgtccg
    10861 aaacagctgc tatacaacct ggctttgttg tttctacaac atattttcca gccctgccgg
    10921 catccattgt aaaattaaaa ctcatggatc ccccggtacc acttacaggg ccattgacag
    10981 ctattccatt ttttttccag gtatattcct ggtcagtttg tgaaaccgcc attccaatac
    11041 ttacggaaga acccaggcaa taaactcctg tacccgtaat accgggaact atggtgccag
    11101 gttctaaaac tgccacatta gaagttacca aacatccact tatcattgat cgtacataat
    11161 atataccacc cgttatggcg gtataagtat caccaaatgc atcgttaatt ttaacatcgt
    11221 ttttatacca ttcataattt ccattagccc cggatgccgt taaaacaatt tgattgctgc
    11281 tgcatatact tcctccatta ggagacaata taatcgtatt ggcacaaggt gtaaaacata
    11341 cagcaccagt aatggaaagg ccatctatgt atgcccccgt acccgtcaca ctattatcag
    11401 taccccggcg ccacctcaat acaacggatt tattatttgc agatacaggc aggtttatgg
    11461 ttgttgttac ccatccgcct gaacttcctg tccacgctga acggttgctt agcggattac
    11521 cgcagcagtt acttattgca gcattataac ctccaactac aaatgaaccc ccggcaagca
    11581 aaatatctgt gaagggcatt cctccaatgg aaatttcaag caccatgcca tcataattca
    11641 gttcaaggtc atgattcctc ctgaatgtaa tttgggcaga tgcagaaact atctggaata
    11701 atcttgaata caggtattca tctgaaacac aatcaggatc attaacaaaa acagaattag
    11761 gcggactgtc tggtgttgag ctagccgtta cccaaggatt tgagttaaca caatccaatg
    11821 cagtagcggc agtccaggct gcaggtaaag cgggagcagt gaccccatca aaattctgag
    11881 tacaggaaag tacaggtgtt gaacaaccag tgatcgtaat attatcaata aaaactcctg
    11941 tagctgatac aatgtgatca gtaccccggc gccatcttaa agcaatgatt ttcccatttg
    12001 cagaagccgg cagcgttaca gtggtagtta caaaagcacc ggatgtagat ccggtccatg
    12061 cccttcttcc tccaattgga ttactaccga accaaagtga tgtagcagga taaccaccgg
    12121 cagtaaagcc cccccccgca gcaaggatat ccacaaaact tcccccatca attgatatct
    12181 ccagtaccaa tccatcataa ttcagttcca attcatgttt tcttctaaac gttaactgtg
    12241 cagcagcact ggtaatagaa aaatattttg aaacaagtac ttcatcggat acacagccgg
    12301 gtgcattgac aaatgcagta ttgatcccat tatcgggagt tgttgtggat gtaacccatg
    12361 gatttgagct gccgcaatct gtcaacgtta ctgcactcca ccctgcaggt aaagctggtg
    12421 ccgtcacagc atcaaaattc tccgtgcatt gagctgaagc cttgttaata acggatataa
    12481 aaaacagaaa aatgactgta caagaaataa aaattctttt catcgttggt ggttgtggtg
    12541 tttgtttcaa aaaatgatca tcgaatcaat atccaggcgg cccatttata cggactatgc
    12601 ctgtacttat ttctcattac agcctgtgct tcgtaaaatg atttatctat tgctttttga
    12661 tcaaatacat ttttataaaa caagtgcatg aactcggctg tttctgcatc tggtactttc
    12721 cagaggctca ttaacaggtt ctctgcccct gccattttaa atgctctctg cagtccatat
    12781 acaccttcac tgcctttaat atcacccagt gccgtttcgc aagcagacag cgcaaccaat
    12841 tttgtattgg gcaggtaaag attcgctact tcataagcgg tcagaattcc gtcctcttcc
    12901 atttctccag gaggtttacc tttccaggta tcgttggcac cggcaaacaa taaccccgac
    12961 cggaataaag gattctccga ttgttgaaat gctttcccac ctaccgaact gtcggcctgg
    13021 ttttctattg tggtcttttg cgggtcatca aagaaaaaac catgtgtagc gatatgtaat
    13081 acacggggag accctttccc tgttaactgt tttacagaat cttccctggc atcccatcct
    13141 ttgatcacta caggggaata tttttttgat cttccggcta gtgctacctg gttcacttcc
    13201 atggctgttc cgggtaaata atcaaaaccg cttctttttg caagaggacc ggtattaacg
    13261 gcaggttttg ttttcggtgc aacctgtgca tactgaacgc caccgaataa ataaatcttg
    13321 tcatccggct ttacataatc agctgtttgc gcagtaacag cggctgtcgt agatacctgg
    13381 aacaattgat acttgtcgct caataaagtg gcgctatcta ccggcaatgc agcaaatgct
    13441 atacgatgta acaaacctgc aggtgcaaaa tagatggtgt ttactccttt tagtttttct
    13501 tccagcggtt tccagatagt attaaatgct aaccgggaaa gactgctttc agtttttact
    13561 tttaatcctc ttgtataaaa cgtattgata ctttgacttc ccgatgccgt ttttctcaac
    13621 aaactgtcga cctgtctttt ttcaaaaagg gtgacatatt ccggttcctt cattcccttt
    13681 cgcaaaacaa gggccgcata aaaaatactg tctgtcgtac gatggccatt aaaataatta
    13741 aactcaataa actctatggc tgcatcagat ggctttagtt gttgttggat atcctcccag
    13801 cttattgatt tttgttgtgc ttttccaaaa gcagatgaac gacgggtaag atctttttca
    13861 agcagatccg ctttttcttt tatacccgct aaatgttcat tctttgccat atctcctttg
    13921 gaatagatcg ctgcaatctg ctttttataa ctgatccagg tatcatacgt tctcgtcagg
    13981 gttgtatcgt tactggtata gatcaattgc cggagttgtt gcgatgaaga aagtatctgg
    14041 ttcctttttt gtaaagagat caggtaaggt tgtcccctgt tgtcccggtt cattttatca
    14101 aaataaaagg attgatattc atcttcagct cctgtaatat ttttcaggta caactgcttc
    14161 tcctcttcag atgtaaagga gaaaagagat gctgactgga gatgttgtgc ttcaaatgct
    14221 tttacataca gctcattggc tttttccacg ttattttttc cccaataaac ccgggcaaga
    14281 cttaatgaat tctctgtata aaatggatga gattctccaa gacgtttttt ccatatttcc
    14341 cgggcccgca aaaagaatt
//
LOCUS       DAORTI010000089        14333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4079_length_14333_cov_5.141897, whole genome shotgun sequence.
ACCESSION   DAORTI010000089 DAORTI010000000
VERSION     DAORTI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14333
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_14333_cov_5.141897"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLO70_11395"
     CDS             <1..266
                     /locus_tag="PLO70_11395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQZ75115.1"
                     /translation="YGAIAECLGTKSSARMVGWAMNGCHHAKLKVPAHRVVNRNGMLT
                     GKFHFSPPEKMEQLLKKEGIKVKNDQVVDFRKHFWNPTEELGF"
     gene            complement(333..740)
                     /locus_tag="PLO70_11400"
     CDS             complement(333..740)
                     /locus_tag="PLO70_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ75116.1"
                     /translation="MQKYFFIRMDGKYIKLCFNDILYAEGSRNYTKIFTETKQYLVLI
                     TMKRLEQFLPTSLFTRIHKSFIVSLEKIVEFDKERVWLQHKELPVGHQYRNELEKSVL
                     IVNDAEYCCLPMANINYVDPIRINGYHQLVEAG"
     gene            complement(838..3777)
                     /locus_tag="PLO70_11405"
     CDS             complement(838..3777)
                     /locus_tag="PLO70_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQZ75117.1"
                     /translation="MRSIKLGLFFLFTSLCYSLGAQQPYTWKQATSNGYTYKYVTNDP
                     MKTRFYTLKNGLSVILSANHKEPRITYRLAVRTGSNNDPSDHTGLAHYLEHLLFKGTD
                     KYGSLDWAKEKPLLDRIEELYEQYISTTDSVKRKEIYKEIDKVSGEAAKFSIAGEYTR
                     MMTALGSQGTNAHTSVEETIYEEDFPSNAIDKFLAIQAERLRQPVFRIFHTELEAVYE
                     EKNRGLDNDGNKMQEAMFSTVFPTHNYGLQTTIGTIEHLKNPSIKAIREYYYKYYVPN
                     NMAIVMAGDFDPDEMIQKITKEFAYLISKPVQEYKGPVEKPIAGPIVKEVFGPSAESV
                     RILFRSAPSGTREALLADLLRSILSNGKAGLIDLNLNKQQKVLGAGAALWQFKDNGIF
                     FLLASPKQGQTLEEVKELLLTQIDILKKGDFDESLIKAIVSNYKLGELQALENNTSRA
                     GNIADEFIKNRGTEWNSNVAAIDEMSKVTKKELVDFANKLLTNNNFVILYKRKGEDKN
                     IAKVEKPAITPVETNAGKASPFVKAVIETPLPSIKPVWIDYNKDLKKEYLGNAEVLYV
                     QNKDNGLFRLTYRFDMGSWNNKLLPIAAQYIQYLGTDKYSSEELSKEFYNLACNFNIN
                     SGTEVTTLSVSGLQENFEKAVGLFEHLINNCRIDEAALTALKNNILKSRANSKLNKNA
                     ISSAMQSYAMYGAKNPFNYVLTDAELQDLKAEDLTNLLHTLSNFQHTITYYGPILIEG
                     LLTSIKKIHKLPATWSKNNSVARFEKAEQTNNTVLFADYDAVQSEIYWIKNLDVYDSK
                     SEPVVNLFNSYFGGDMGSIVFQTIRESKALAYSTYAFVMTPSKKDDKFSMVAYVGSQA
                     DKMNDAVKGMNELLNEMPKSDQNFENARTSLLKNIETDRVTQDGIILNYLNAKRKGLD
                     HDIRKDNYSLYKTLLWNDIYKYHQQEFVKQPYTYCVVASEKRINLEDLKKYGELKILN
                     LTELFGY"
     gene            complement(3909..5096)
                     /locus_tag="PLO70_11410"
     CDS             complement(3909..5096)
                     /locus_tag="PLO70_11410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ75118.1"
                     /translation="MKKNSIIFLSAVLFMMACNNSKNQNEGYVIKGTLKGIDSGMVRI
                     SSYNEDDRTSKTLDSTTFHGGTFELKGNAAPQMVSVNIEPGNWSFQVFLEDTVLNITA
                     DTTGSQYYDQTMYGGYKGARIKNFTATGSKNFDDWMQYQNDPEQKKYDPLFAELGKKI
                     QTTKDIDEQYKYRDQIDSVSKILNQYKSNYVKDFISKNPSSVAGAYMLRDLYIWNSDL
                     PFADMQNMVAGFNGAAKESKYNKSLENILAMRKAVMPGSEAPDFTLMKRDSSKFTLSS
                     TRGKYIMIDFWASWCHPCREAIPHWKEVYNKYHDKGFDIVSVSDDDKWANWFKAMDQE
                     KMPWMQVCDEFPVKNMPSKVGSLYMTTYMPFYVLLDKEGKVLVHSGDEKKIDEKLKEI
                     FGS"
     gene            complement(5196..7496)
                     /locus_tag="PLO70_11415"
     CDS             complement(5196..7496)
                     /locus_tag="PLO70_11415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQZ75119.1"
                     /translation="MFAKIKYLIVFLLFVSMASSAQNIKFEGKSDKKFDGNKVIIYNQ
                     SGLHDSAVIKDGKFDFNVPFNEPALFMFYSRLESKNKGGYSPFSILVTAPGAIKIKAD
                     AENFNESKVSGSKENDLYKNFAEKSGKARQKIINDLENKYGKEFVMSRNPDTSSAKYK
                     ELIAEYNTLNEASQKEQLIDLQQFIHSNPETFSSILFLNNYATTIDMAELESMYESLS
                     PKYKDTKAGKSIVSTINARKITAIGKTAPDFTQADTSGNMVKLTDFRGKYVLVDFWAS
                     WCGPCRAENPNLVKTFNEYKDKGFTVLGVSLDQPGKKEAWLKAIHKDELTWTQVSDLK
                     YWDNEVAVLYGIKAIPANLLLDPQGRIIAKDLRGEDLEKKLSEVIAKTQEKPFTIDGT
                     IKENAGTHIYLSLPDGKTGKTKMDSSLIKNGQFQFAGVLSGPAQVYVTTVRQPRSINE
                     YVQLYIEPGEMKLSLDGKNLSETAVLKGSGVQDEADKLNTLKAAVLAQMKPLSEAYSK
                     ANGIYAAAMREKKDEATLEKLKDEAEKAKEAMYPYQKQLGEIETKFMNDNPASYVTAS
                     MLRYRISSMPLEEGDERYSKLSEIIKSSSLGQEIKKELDGLRMGSPGATASLFSSTEL
                     RGKMLSLSDYKGKYVLLDFWASWCVPCRKGNPHLLKLYSKYKDMGKGLEIIGVSDDDS
                     KPEAWKKAVEKDGIGVWKHVLRGLKWVDGPAMFDRTNDISEGYGIHSLPTKILIDPNG
                     IIIGRYDGGSEDDEAIDKKLAEIFGS"
     gene            complement(7569..8063)
                     /locus_tag="PLO70_11420"
     CDS             complement(7569..8063)
                     /locus_tag="PLO70_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75120.1"
                     /translation="MKLLMFAVLLFQLFSVKGQSAFDKRIAETVEHLACTDPDERRPV
                     TLNAQLVKEGDSIAIIMKIAIASGWHIYAYVPPEQPYIMIEPILRVPEHIKISGEWIK
                     TEPEASSIDKGVLMYENEAVFIRKAVKSDKALEGIIKTGLYYQTCNLRQCLPPELIML
                     ELKY"
     gene            complement(8060..9574)
                     /locus_tag="PLO70_11425"
     CDS             complement(8060..9574)
                     /locus_tag="PLO70_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75121.1"
                     /translation="MKNTYWFLCLIFSFCLGCGINAQPTHSKSNLKVLFVGYDPAKPL
                     PEMDRSAPGMMSKEAFTKEYPIRMPAFKKLLSEYFAQVQTMDCRDWKAANSNPYDVTI
                     FDFRTTPLEAARTEKGADGKTKYISARYLPDNFSKPVIFISSTADEMGRSIGLKLDWL
                     CLCLDANAHHINTQHSIFKGPLEKVTPTMEMKKTPDGIFHYTTGDKIPKEIPMWKVQN
                     VGYLENNNTRIGLVSRGNRFNESPDVEDISSGVCTKDVGAVALGRHGNFFLWGFGASP
                     VDMTDEAKKVFVNAVAYMKQFDGKIPIARKYNDRMATTDDVREIVANATKEKYEEYIK
                     LIEEFNDYNAKEKKRIDEKKTSGQALTSSEEQSLIYLGHPEKINAYEQFLKGIMGKFS
                     AQFGTDAEAFQKYITDNFGYIYCDPNGFYEYSIDEDVQSIGISNHSVKMLEKCVEMLK
                     QNDRPDLALRILKKYTTEGFETAKEWSDWLSKNKNKLFFTETDGYRFMINTYKG"
     gene            complement(9722..11146)
                     /locus_tag="PLO70_11430"
     CDS             complement(9722..11146)
                     /locus_tag="PLO70_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ75122.1"
                     /translation="MKKLFIYIVFATAVSTQQSCKKDLNALPESSRVAESVITDGKTA
                     EIALNGAYYNFANANPRKTSWQYHELPPACLAGYMGYAFGLLPEEENMNASLAFTDYW
                     GESYRLLNTVNNALKGISSLADGDFAGDRKKEIIGELHFLRAYAHFKLFSYYGVWFDI
                     NSPLGVLLRDEASTKANISKARSTVKDSYDFILADLDDASANAASARPNYYATKWAAM
                     VLKMRVLMSRGAAGDHAEIITLANTIIQTSPYQLEPNLVDIFHSKGLASNEVILGLKP
                     QANQEQDYYSRSYQYWPFHSAGFVAKGALKDLLTGDPRQAWMIGSANPYSAYSPNTFF
                     FTKYMIENGTPTTVSETYYAIRLSEVYLLRAEAIVRSGGSLATARTDVKNVMSHAGVI
                     DFTAVDNAGTPEDLLLQIYYETVRNLVTEDGQEWMALLRLDFNTVKLLKPTITKKEQY
                     IIPIPHSEFVYNPEIGDQNPGYDK"
     gene            complement(11166..>14333)
                     /locus_tag="PLO70_11435"
     CDS             complement(11166..>14333)
                     /locus_tag="PLO70_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75123.1"
                     /translation="IVLKTAVVKRTDPIIEEVPPQANINGTVKDTDGKPLASVSVSVK
                     GTNAGTTTNSKGEFSLSGIDANATLIFSIVGYATQTVSLNGRNNINITLAVDAKQQSE
                     IVVMGYGTLKRKDLTGSVSSVNTEEIKNTPFVSIDQALTGKAAGVQVIQGDGSPGGMA
                     KIRIRGGTSLLGGNDPLYIIDGIPVQVQNRYLQSAAEIVSPVERFGNGDADNTISGSF
                     TRGLNSLGGLNINDIETIDILKDASATAIYGSRAANGVVIITTKKGKTDQKPILEANY
                     YAGLSVPIKEKLLNAEQYVAIMKEGAKNLNDARAAANPVRPPDALATSILNTPGFLGT
                     ANTDWLDLVLRNGFSQNADISVRGGGMSSRYYTSLAYTRQNGVVVGTDFQRISGKVNM
                     DNEITNRLRILSNMDYSFTKNNITNGMYTQALYAPPTLPAYNPDGSVHVITAADLGAQ
                     SYQGYQNPLLLLNGKNKSNTLQLLGSLALEYDLLKSLKFKSLVSVNYANYHQDNYTPS
                     TVMISSASGTQTTPGIATQAQSQNANVFFENILTWDKQFNANNRINLVAGSSWQKSRM
                     NSFSASGQGFPDDVYLNNLSSAALALPPSGSSGQSSLLSFYMRVNYALKEKYLFTFTG
                     RSDISSKFPQNNRTGYFPSGGVAWRMSEEKFLKNVSWINELKLRASAGYTGTQNIADN
                     LFYTLYNPASYGGTNALIPSQLGNEELKWETTLQKDAGIDIALFNSRLKASFGLYEKT
                     TDGVLYTTTVASSSGFNSLISNIAKIRNRGLELDFRGDFIRNKNFQWTGALNISKNRS
                     KVLAISNDLAVQDNGVVQFGNTSLVVGQPLGVFYGRKFLGLLKTQKEVDDYKAANLLA
                     AYGFYPYLAIGDPYYLLDAPVFGYFKDTVIGHAEPKFFGGFTNTFNYKNFSLIALLTY
                     SYGGDIFYEADIQNHDLGNRTNKSTRILDHYSPENPNSEFPRILLGQSPSVYTASNNV
                     YDASFIKLKSITLNYEFSRKVLDKAKIRSASIYLSASNLFTITKYPGPDPEVSNDPYS
                     IIGGYTDVAGYPTIKQYNVGIRVGF"
BASE COUNT         4046 a         2963 c         2461 g         4863 t
ORIGIN      
        1 cttatggtgc tattgcagaa tgtctcggca caaaatcttc tgcccgcatg gttggttggg
       61 caatgaacgg ttgtcatcat gcaaaactga aagtacctgc acatagagtt gtaaacagaa
      121 atggaatgtt gactggaaaa tttcattttt ctcctccgga gaaaatggaa caattattaa
      181 agaaagaagg tatcaaagtg aagaatgatc aggttgttga ttttagaaaa catttctgga
      241 acccaacgga agagcttggt ttttaaaata gcaacaaaaa aaggagtgct ttcacacccc
      301 tttcacacca aactaactgc aaatgagaaa atttatccag cttcaactaa ctgatgatag
      361 ccgtttattc taataggatc aacgtaatta atattcgcca ttggcaagca acaatactcc
      421 gcatcattaa caataaggac cgacttttct aattcatttc tatactgatg acctacaggt
      481 agttctttat gttgaagcca cactctttct ttatcaaact ccacaatttt ttccagtgac
      541 acaatgaacg atttatgtat ccttgtaaac aaactagttg gtaaaaactg ttcaagtctt
      601 ttcatggtta taagcaccag gtactgtttc gtttcagtaa atatcttagt gtagtttctt
      661 gaaccttcgg catatagtat gtcgttgaag catagtttta tatacttccc atccattcgt
      721 ataaagaaat atttttgcat aagatcaatt ttttgagacg tttatcttaa tggtatccgg
      781 tatagccaac agtgcaattc tcatcttatt tacttgcctt tattatttaa gattctttta
      841 atatccgaat aactctgtca ggtttaaaat ttttaattcg ccatactttt tcaaatcctc
      901 caggtttatt cttttttcag aagccaccac gcaatacgta tatggttgct ttacaaattc
      961 ctgctgatgg tatttataaa tatcattcca cagtagtgtt ttgtacaatg aataattatc
     1021 tttccggata tcatgatcca aacctttacg tttggcattc agataattta atataatacc
     1081 atcctgagtc accctgtcag tttcaatgtt tttcagtaaa cttgttcttg cattttcgaa
     1141 attctgatcc gatttaggca tttcattcaa caattcattc atacccttaa cagcatcatt
     1201 cattttatct gcctgacttc ctacataggc aaccatagag aatttatcat ctttttttga
     1261 gggtgtcatc acaaatgcat aagtagaata ggctaatgct tttgattctc ggatagtttg
     1321 aaagacaata gatcccatat ctccgccaaa ataactgttg aacaggttaa cgacaggttc
     1381 agattttgaa tcatagacat caagattctt tatccaataa atctcagact gcactgcatc
     1441 ataatctgca aaaagaactg tattgttcgt ctgctctgct ttttcaaacc tggcaacact
     1501 attgtttttg ctccaggtag ccggtagttt atgaatcttt ttaatgctgg ttaataaacc
     1561 ttcaattaaa atgggaccat agtaagttat tgtatgttga aagtttgaca atgtatgcaa
     1621 caggtttgtc aggtcttctg ctttcaggtc ttgtaattca gcgtcagtca gtacataatt
     1681 aaaaggattc tttgcaccat acatagcata tgattgcatt gctgaagaaa tagcattctt
     1741 gtttagttta gaattggctc ttgactttag aatattattc ttcaaagctg taagggcggc
     1801 ttcatctatt ctgcaattat taataaggtg ttcaaataaa ccaacagctt tttcaaaatt
     1861 ctcctgtaat cctgaaactg aaagcgtcgt aacttctgta cctgaattaa tattaaaatt
     1921 gcaggcaaga ttatagaatt ctttgctcaa ttcttctgac gagtatttat ctgtgcccag
     1981 gtattgaata tattgagcgg ctattggtaa tagtttatta ttccagctac ccatatcaaa
     2041 acgataagtt aaacgaaata aaccattgtc tttattctga acatataaca cttcagcatt
     2101 gcccaggtat tccttcttca aatctttgtt ataatcgatc caaactggtt ttatagaggg
     2161 taaaggtgtt tctataacag cttttacgaa tggtgatgct ttgcccgcat tcgtttctac
     2221 tggtgtaatt gctggttttt ctacttttgc gatattctta tcttctcctt ttcttttata
     2281 aagtattaca aaattattat tggttaatag cttgttggcg aaatcaacta attccttttt
     2341 agtcacctta ctcatttcat caatggctgc tacattacta ttccattcag ttccccggtt
     2401 ttttataaat tcatcagcaa tattgcctgc cctgctcgta ttgttttcca atgcttgcaa
     2461 ttctcccaac ttataattgg atacaattgc tttaataagg ctttcatcaa agtctccctt
     2521 ttttagaatg tcgatctgtg taagtaaaag ttcttttact tcctccaggg tttgtccctg
     2581 ttttggagaa gccagtagaa agaatatgcc attatcttta aactgccaaa gggctgctcc
     2641 tgctcctaaa accttttgtt gcttatttaa gttaagatcg atcaatccgg cctttccatt
     2701 agaaagtatc gatctaagta ggtcggccag taatgcttct cttgtacccg atggggcaga
     2761 acgaaatagt atgcgtacac tttcagcact ggggccaaaa acttctttca ctattggacc
     2821 tgcaattggt ttttcaaccg gtcctttata ttcctgcaca ggttttgaaa ttagataagc
     2881 aaattcttta gttatctttt gtatcatttc atcaggatca aaatcccctg ccattactat
     2941 ggccatgtta ttgggaacat agtatttata atagtactca cggattgcct ttatggatgg
     3001 atttttcaga tgttctatgg tgccaatcgt tgtttgcaat ccatagttgt gtgttggaaa
     3061 aacagttgaa aacatagcct cctgcatctt atttccatca ttatccaacc ccctgttttt
     3121 ctcttcataa acagcttcca gttcggtatg aaatattctg aatactggct gtcttaaacg
     3181 ttcagcctgg attgccagaa atttatcaat tgcgttagaa ggaaaatcct cttcatagat
     3241 ggtttcctca acagaagtat gtgcattagt tccttgtgaa ccaagggctg tcatcatacg
     3301 ggtgtactca ccagcaatag aaaacttagc ggcttccccg gaaactttat caatctcctt
     3361 ataaatctct ttacgtttta ctgaatctgt tgtggaaata tattgctcat acagttcttc
     3421 gatcctatca agtaaaggtt tttctttagc ccaatccagc gaaccatatt tatctgttcc
     3481 tttgaataac aggtgttcga ggtaatgagc cagtcctgta tgatcacttg gatcattatt
     3541 actaccggta cgtaccgcta accggtaggt aattcttggt tctttatgat tagcggacaa
     3601 aattaccgac agaccgtttt ttaaagtata aaaacgggtc ttcatcggat cattggtcac
     3661 atacttatat gtatagccat ttgaggttgc ctgcttccag gtgtagggtt gctgggcgcc
     3721 taagctatag cacagacttg taaataagaa aaatagaccc aattttatac ttcgcataat
     3781 cgatgattaa aaattacatt cgtttccgaa attttattga actttttgat ttacacattc
     3841 attgaattaa taagagaaaa caattcaatg cattttaatg ttctatgatt cccttttttg
     3901 atagcttttt aacttccaaa aatttctttc aatttttcat cgatcttttt ttcatcgccg
     3961 gaatgcacta atacctttcc ttccttatca agcaatacat aaaatggcat ataagtggtc
     4021 atatatagcg aaccaacttt tgacggcata ttctttacag gaaattcatc acatacctgc
     4081 atccacggca ttttttcctg gtccattgct ttaaaccaat ttgcccattt atcatcatca
     4141 gacacactta cgatatcaaa acctttatca tgatatttat tatacacttc tttccagtgt
     4201 ggaatggctt cacggcatgg atggcaccag cttgcccaga aatcaatcat tatatatttg
     4261 cctcttgtgg aagacaaagt aaattttgaa ctatctcttt tcatcaatgt aaaatcaggt
     4321 gcttcacttc ctggcattac agctttacgc attgccagaa tattttctaa acttttattg
     4381 tatttagatt cttttgcagc tccgttaaag cctgctacca tattttgcat atccgcaaat
     4441 ggcaaatcag aattccagat ataaagatcc ctaagcatgt atgcacctgc gacagaagaa
     4501 gggtttttac tgataaaatc tttaacgtaa tttgatttat attgattcaa tattttactc
     4561 acagaatcta tctggtctct atatttatat tgttcatcaa tatctttagt agtttgaatt
     4621 tttttaccca attctgcaaa taaaggatca tatttttttt gttccggatc gttttgatat
     4681 tgcatccaat catcaaaatt ctttgaaccg gttgctgtaa aattttttat cctcgctcct
     4741 ttgtagccac catacatagt ctgatcgtaa tattgagatc cggtagtatc ggctgtaatg
     4801 ttcaatactg tatcttctaa aaatacctgg aacgaccagt tgccgggttc aatattcact
     4861 gaaaccatct gcggggcagc gttacctttt aattcaaaag ttccaccatg gaaagtagtg
     4921 ctatcaagtg ttttagaagt tctgtcatct tcattgtaag aagatattct taccattcca
     4981 gaatctatgc cctttaatgt tcctttgata acatatcctt cattttgatt tttgctgtta
     5041 ttacaagcca tcataaacaa aacggcactg agaaatatga tactgttttt tttcatagtt
     5101 gattttgttt aaaattattt ttttaactat cgtaatatta ttcccaaaat aattctgcca
     5161 attttgtatt tgagtgcttc tacctataca gcagtttagc ttccgaaaat ctccgccagc
     5221 tttttatcta ttgcctcatc atcttcgctg cctccatcat accggccaat gattatacca
     5281 tttggatcta taagtatttt tgttggcaga gagtggattc catatccttc agaaatatca
     5341 ttagtgcggt caaacatagc aggaccatct acccacttta ggccacgcag cacatgtttc
     5401 catacaccaa ttccatcttt ctctacggct ttcttccagg cttccggctt gctatcatca
     5461 tccgatactc ctattatctc aaggcccttt cccatgtctt tatatttgga gtaaagtttt
     5521 aacaggtgtg ggttgccttt tctgcaggga acacaccaag atgcccaaaa atctaataac
     5581 acatacttgc ccttataatc cgaaaggctc aacatttttc cccggagttc tgtagaagaa
     5641 aagagtgaag ccgtagctcc aggtgagccc atgcgtaatc catcaagttc tttttttatt
     5701 tcctgaccaa gactgctgct ctttattatt tccgatagtt tactataccg ttcgtcccct
     5761 tcttccaaag gcatactgct tatgcgatag cgcaacatag aagccgtaac ataagatgca
     5821 gggttatcat tcatgaattt tgtttcaatt tctcctaatt gcttttgata cgggtacatg
     5881 gcctcttttg ctttttcagc ttcatccttg agtttttcca gagtggcttc atctttcttt
     5941 tctctcatcg ctgcagcata aataccattt gctttactat aggcttcaga cagtggtttc
     6001 atctgagcta acacagctgc tttcaaggtg ttgagcttat ctgcttcgtc ctgaacgccg
     6061 gagcctttta gtacagcagt ttctgaaaga tttttcccat caagtgaaag tttcatttcg
     6121 ccgggttcaa tatataattg tacatactca ttaatagatc ggggttgcct tactgtcgtt
     6181 acatacacct gtgccggacc acttaatact cctgcaaatt ggaactgtcc atttttaatt
     6241 aaggaactgt ccattttcgt tttcccggtt ttaccgtcag gcagggataa ataaatatgg
     6301 gtacctgcat tttctttaat cgttccgtcg attgtaaaag gcttttcctg ggtctttgca
     6361 ataacttcag ataatttttt ttccaagtct tcgcctctta aatccttggc gataattctt
     6421 ccttgtgggt caagcaagag gttagctgga atggccttga taccatagag tacagctact
     6481 tcattgtccc agtatttgag atcagaaacc tgtgtccagg taagctcatc tttatgaata
     6541 gcctttaacc atgcctcttt ttttccgggt tgatctaagg aaacacctaa tacagtaaat
     6601 cctttatcct tatactcatt aaaagttttt accaggtttg gattttctgc acggcaaggc
     6661 ccacaccagg atgcccagaa atcaaccagc acatacttgc cccggaaatc agtcagcttt
     6721 accatatttc ccgaagtatc agcctgggta aaatcaggag cagttttgcc aatagctgtt
     6781 atcttcctgg catttatcgt agaaacaatg gatttaccag ccttggtatc cttgtatttt
     6841 ggagacaagc tttcatacat gctctccagt tctgccatat ctatcgttgt tgcatagttg
     6901 tttaaaaata atatggaact gaaagtttcc gggttgctgt gaatgaactg ctgaagatct
     6961 atcaactgct ctttttggct ggcttcattt aatgtattgt actctgctat caactcctta
     7021 tactttgcac tggaagtgtc tgggttacgg ctcattacaa attctttacc atatttattt
     7081 tccaggtcat ttatgatctt ctgccgggcc ttaccagatt tctctgcaaa atttttgtag
     7141 aggtcattct ctttcgaacc gctaactttt gactcattga aattttcagc atctgcttta
     7201 attttaatgg ctccgggtgc tgttactaat attgaaaaag gagaatagcc tcctttattt
     7261 ttactttcca atcggctgta gaacataaaa agtgccggct cattaaaagg cacattgaaa
     7321 tcaaacttgc catctttaat tactgcagaa tcgtgtaagc cactttgatt atatattatg
     7381 accttattgc catcaaattt tttgtcggat ttcccttcaa atttgatgtt ctgtgccgag
     7441 gaagccatgc taacaaatag caggaagaca attagatatt ttatttttgc gaacataatt
     7501 tttatttaaa gattgctaat taaaaaattt atttttttgt ttctttctct aaagcatctg
     7561 ccaataaatt agtatttcag ttcaagcatt attagctcag ggggaagaca ttgtcttaaa
     7621 ttacaggtct gataatatag tccagtcttt ataatccctt ctaatgcttt atctgattta
     7681 actgccttgc gaataaatac agcttcgttt tcatacatca aaacaccctt atcaattgaa
     7741 gatgcttccg gttccgtttt tatccattct ccactaattt taatatgttc cggcacccgg
     7801 agaatcggtt caatcataat gtatggctgt tccggcggta cgtatgcata aatatgccaa
     7861 cccgatgcta tggctatttt cataatgatg gcaatgctat ctccttcttt taccagctgc
     7921 gcattaagtg ttaccggcct tctttcatca ggatctgtgc aagctaagtg ctcaacagtt
     7981 tccgcaatcc ttttgtcaaa cgcagactgt ccttttacag aaaataattg aaatagtaaa
     8041 acggcaaaca ttaataattt catcctttgt aggtattaat cataaaacgg tatccgtcgg
     8101 tttcagtaaa aaatagtttg tttttatttt tagaaagcca atcactccat tcttttgcgg
     8161 tctcaaaccc ttctgttgta tattttttca atattcgtaa agcaagatcg gggcggtcat
     8221 tttgtttaag catctccaca catttttcca gcatcttcac actatgatta gaaataccta
     8281 ttgattgcac atcctcatca atagagtatt cataaaaacc attcggatca caatagatat
     8341 acccaaaatt atctgtaatg tatttttgaa aggcttcagc atcagtaccg aattgcgcag
     8401 aaaattttcc cataattcct tttaaaaact gttcataagc atttatcttt tccgggtggc
     8461 ctagataaat aagggattgt tcttctgaag aagtaagtgc ctggccggag gtttttttct
     8521 catctatcct ttttttctcc tttgcattat agtcgttaaa ttcttctatt aattttatat
     8581 attcttcata tttttctttc gtggcatttg caacgatctc ccgcacatca tccgtagtgg
     8641 ccatgcggtc attatatttt cgggcaatag gtattttgcc gtcaaactgt ttcatataag
     8701 ccaccgcatt tacaaaaacc ttttttgctt catctgtcat atccaccggc gaggcaccaa
     8761 agccccataa aaagaaattg ccatgcctgc ccagcgctac cgcacctaca tcttttgtac
     8821 atacgccact ggaaatatct tctacatctg gtgactcatt aaacctgttg ccacgggata
     8881 ccaggcctat acgggtatta ttattctcta ggtatcctac attctgaacc ttccacatcg
     8941 gtatttcttt tggaatttta tcaccggttg tataatggaa aatcccatcg ggtgttttct
     9001 tcatctccat tgtaggtgtc actttttcaa gcggcccttt gaatatactg tgctgggtgt
     9061 tgatgtgatg tgcatttgca tccaggcaca ggcaaagcca gtcgagctta aggccaatgc
     9121 tacggcccat ttcatcagcc gtgctggaaa taaaaattac tggtttagag aaattatcgg
     9181 gaagatatct tgcagatata tactttgttt taccgtccgc acccttttct gttctggcag
     9241 cttctaaagg agttgttcta aaatcaaaaa tggttacatc ataagggttg gaatttgccg
     9301 ctttccaatc ccggcaatcc attgtttgta cctgcgcaaa atattcgctc agtaattttt
     9361 taaaggctgg catccttata gggtattctt tggtgaatgc ctctttgctc atcattccgg
     9421 gggcagacct gtccatttca ggaagtggct tcgccggatc gtaacccaca aaaagaactt
     9481 tcaggttact ctttgaatga gttggctgtg cgttgatccc gcatccaagg caaaaagaaa
     9541 atatcagaca caaaaaccaa taagtgtttt tcatattttt aattaattgc tttaaaaatt
     9601 tatttctgtt gtattcatac aacattttga taaataagaa tgcacaattt cacctgcgga
     9661 ttatgaagaa tattaaaagg gatcaggaag tcccctttaa tattcatgaa gacattcaat
     9721 tttatttatc ataacccgga ttctgatcac caatctccgg attatataca aattcactat
     9781 gtggaatggg gataatgtat tgttcctttt tagtaattgt aggttttaaa agttttactg
     9841 tattaaagtc caaccttaat aaagccatcc attcctggcc atcttctgtc accaggtttc
     9901 ttactgtctc ataatatatc tgcagcaaca aatcttcagg agtgccagca ttatcaaccg
     9961 ctgtaaaatc aataacgcct gcgtggctca ttacattttt tacatctgtc cttgcagtgg
    10021 cgaggcttcc accagacctt actatagctt ctgctctcaa taagtaaacc tcagacaggc
    10081 gtattgcata gtaagtttca gaaaccgttg ttggcgtacc attctctatc atatatttag
    10141 taaagaaaaa tgtattgggg ctgtatgctg aatatggatt ggcgctaccg atcatccatg
    10201 cttgccgcgg atctcctgtc aaaagatctt tcaaagcccc tttagcgaca aaacccgcag
    10261 agtggaacgg ccagtattga tagcttctgc tatagtaatc ctgttcctga ttggcctgtg
    10321 gtttcaggcc taaaataact tcgttacttg ccagtccctt tgaatggaaa atatctacca
    10381 aattcggttc caactggtaa gggctggttt gaatgatagt attggcgaga gtgataattt
    10441 ctgcatgatc accggccgca cctctgctca ttaaaactct cattttcaaa accattgccg
    10501 cccatttggt tgcataatag tttggccttg ccgaagctgc attagctgaa gcatcatcaa
    10561 gatcagcgag tataaaatca taactatctt ttacagtact ccttgctttg gaaatatttg
    10621 cttttgtaga agcttcatcc ctcaacaaaa cgccaagcgg actgttaata tcaaaccata
    10681 ctccgtaata gctaaataat ttaaaatgtg cataggcccg caggaagtga agttcgccga
    10741 taatttcttt ctttcggtca ccggcaaaat caccatctgc aagtgaactt atacccttaa
    10801 gagcattatt tacagtattt agcaatctat acgattcacc ccaataatcg gtgaatgcta
    10861 aacttgcatt catattttct tcttcaggca ataagccaaa agcatacccc atataacctg
    10921 caaggcaggc tgggggaagt tcatggtatt gccagcttgt ttttctagga ttggcattgg
    10981 cgaaattata gtaagctccg ttcaatgcaa tctctgcagt ttttccatcg gtaattaccg
    11041 attcagctac tcttgaactt tcggggagtg catttaaatc tttcttgcag gactgctgag
    11101 tggaaacggc agtcgcaaaa actatatata taaataattt tttcattttt acttttttta
    11161 atggtttaaa atccaactct tattccgacg ttatactgct ttatagtagg ataaccagct
    11221 acatcagtgt atcctccaat aatgctgtac ggatcattgg aaacctcagg atccggaccg
    11281 ggatattttg taatggtgaa caggttggat gctgaaagat atatagatgc acttctgatc
    11341 ttggctttgt cgagtacctt ccgtgaaaat tcataattca gggtaataga ctttaattta
    11401 ataaaagaag catcatacac attattgctg gcagtataaa cggaaggaga ttgtcccagc
    11461 agtatccttg gaaattctga gtttggattt tccggcgaat aatggtccaa tatcctggta
    11521 cttttgttgg ttctgttccc cagatcatga ttttgaatat cggcttcata aaatatatct
    11581 ccaccataag aataggttaa taaggcaatg aggctaaagt ttttatagtt aaaagtattg
    11641 gtgaaaccac caaaaaattt aggttctgca tggccaatga ctgtatcctt aaaataacca
    11701 aataccggtg catccaataa ataatacgga tcaccaattg ctaaataagg atagaagcca
    11761 taggctgcca gcaggttagc agctttgtaa tcatccacct ctttttgagt cttcaataac
    11821 cctaagaatt ttcttccata aaacacacct aatggttgac ccactacaag tgatgtattt
    11881 ccaaactgta ctaccccgtt atcctgtact gcaaggtcgt tggaaatagc cagcacttta
    11941 gaacggttct tagaaatatt gagtgcacct gtccattgaa aatttttatt gcgaatgaaa
    12001 tcgcccctga aatccaattc caggcctcta ttcctgatct tggcaatatt agaaattaag
    12061 ctattaaatc cggaactact tgcaacggtt gtggtgtaca aaacaccatc tgtagttttc
    12121 tcataaagcc caaaggaggc ttttagccgg gaattaaaca aggcaatatc aattccggca
    12181 tctttttgca gagtggtctc ccattttaac tcttcattcc cgagctgtga aggaatcagt
    12241 gcattggtgc ctccgtaaga agcaggatta tataatgtat aaaacaaatt atcagcaata
    12301 ttttgggtac ctgtatagcc agcacttgcc cggagcttca gttcattgat ccaggaaaca
    12361 tttttcagaa atttttcttc tgacatgcgc catgctacgc ctccggatgg gaaataacct
    12421 gtccggttat tttgagggaa tttagaagag atatcagagc ggcctgtgaa ggtgaataag
    12481 tacttttctt taagagcata attcactctc atataaaaac ttagcagtga actctgacct
    12541 gaagaacctg acggaggaag ggctaaggca gccgaggata aattatttaa atatacatca
    12601 tccggaaatc cctggccact ggccgaaaaa gaattcatac ggcttttttg ccaggagcta
    12661 ccggccacta aattgatccg gttattggca ttaaattgtt tatcccaggt taggatattt
    12721 tcaaaaaaca cattggcatt ctgcgattgt gcctgtgtgg caatacccgg tgttgtttga
    12781 gtaccactag cagaagatat cattaccgta gaaggagtat aattgtcctg gtgataatta
    12841 gcatagttta cggaaaccag cgatttaaat tttaatgatt tcaacaaatc atattctaat
    12901 gctaacgaac ccaataattg aagcgtattc gatttatttt ttccattgag taaaagtaat
    12961 gggttctggt atccctgata gctctgagca cccaggtcag cagcagtaat aacatgcaca
    13021 cttccatccg gattgtaggc cggcaaggta ggcggtgcat atagggcctg ggtgtacatt
    13081 ccattggtga tattattttt cgtaaagcta taatccatat ttgaaaggat cctgagccta
    13141 ttagtaatct cattatccat attgacctta ccagatatcc tttgaaaatc tgtaccaact
    13201 accaccccgt tttggcgagt ataggcgagt gaagtataat accgtgaact cataccaccg
    13261 cctcttacag aaatatcggc gttttgagaa aatccattcc ttaataccaa atcaagccaa
    13321 tcggtattag cagttcccaa aaagccaggt gtattaagta tgctagttgc caaagcatca
    13381 ggaggacgta ctggattcgc cgctgctctt gcatcgttta aattcttcgc tccttccttc
    13441 ataatggcga catactgctc tgcatttaac aacttttctt tgataggaac acttaagcct
    13501 gcataataat tggcttccag aatgggcttt tggtcagttt ttcccttctt tgttgttata
    13561 atcacaactc cattagctgc cctagaacca taaattgcag tcgccgaagc atcttttaaa
    13621 atgtctatgg tctcaatatc attaatattt aatccgccca ggctattcaa acccctggta
    13681 aaagatccgg aaatggtatt gtcagcatca ccattaccaa agcgttctac cgggcttaca
    13741 atttcggctg cactttgcag atagcggttc tgtacctgaa ctggaattcc atctatgata
    13801 taaagaggat cattaccgcc caacaaggaa gtacctcctc ttatgcggat ctttgccatt
    13861 ccaccaggag aaccatctcc ctgtataacc tgtacaccag ccgcctttcc ggttagtgcc
    13921 tggtcaatac ttacaaaagg tgtatttttt atttcttctg tatttacact tgacactgaa
    13981 cctgtcaaat cttttctctt caatgttcca tatcccatta ctacaatttc actttgctgt
    14041 tttgcatcaa cagccaaagt gatatttata ttatttcttc cattcaatga aacagtttgg
    14101 gtggcataac caactattga aaaaataagt gttgcattcg catcaatacc tgacagagag
    14161 aattctcctt ttgaattagt agttgttccg gcattggttc ctttcacaga tacagaaaca
    14221 cttgctaacg gttttccatc agtatctttt acagttccat ttatatttgc ttggggcggg
    14281 acttcttcaa taataggatc tgttctttta acaacagcag tcttaagtac aat
//
LOCUS       DAORTI010000090        13822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4306_length_13822_cov_7.679160, whole genome shotgun sequence.
ACCESSION   DAORTI010000090 DAORTI010000000
VERSION     DAORTI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4306_length_13822_cov_7.679160"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLO70_11440"
     CDS             <1..1061
                     /locus_tag="PLO70_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75124.1"
                     /translation="DTVLHTLSWLFESLDPVTRELVADPNDGFLNPNQQSPEGEGFVS
                     FSVRPLANRPHLQQVMNTAKIVFDENAPIFTNVWKNTVDIEKPSSQVLPLPAVLNDTT
                     FLVRWSGTDAHAGIMGYDIYAIINDTITRRILRNTPTDSIRINGAFGNTYKFFSVASD
                     LVGNIEDLPGVPDAVITLSAPLPLTILSFTGSRQNNDAVLQWRTVNEVSVSHFEVQRS
                     FNGADFTKEGTVTAGGTLYSLTDPGVFNTQPVVYYRLKSVDIDGRFTYSAILKLSRNE
                     NGLLTVFPNPVKDQLVIGGLKRGAVLRIFSADGRLVQQTAVTTQTMIIDMIGYTSGIY
                     WLQYASDGEIQTQKILKQ"
     gene            1402..2925
                     /locus_tag="PLO70_11445"
     CDS             1402..2925
                     /locus_tag="PLO70_11445"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQZ75125.1"
                     /translation="MRCQLMLFSGLLFIALSGSAQSACDESANGYTIPAKEINCFRHF
                     SSNQQPGVHAVNNYLLAKISQLMYTERLDFELRKLRNPSGFPAADFSSSQLNSSSNNS
                     FECDFKKRFSHWFYDVNSKPVRPLKIDKNLQMAPVENRNKPGVVNNSNLKSNQMLNDK
                     ADLSGKARVIENKNTTTMSPEQKFIQDSVAFEKSKPKFKFLNKRTDFVNIGGEIRIPG
                     FDPELMVISTEEYIIVAWRGTDNVYRDDSWEWIGTDAYFVPVSGDGPLASAKMHAGFW
                     HSFKVIRNKLMATLDAFEAKSKNKKIFITGHSLGGGMAMISAPYLAGTGYKVAEVYTY
                     AAPRVIGDQAFVNRCNTLLGSDKIQRFEYGVDFVTKLWSPAIYFSTYKIPGHRHWLNA
                     QGADDDYNCGERYFPMTANPFEYNDYSRAKIDKLNGDVGAFDPFGWAALFKTSIKGQS
                     PKYPKESHSYPLFDFGQHNPQYYVKKAFENMTTEQKGLLPGFQDTYPYVYPAVPGNK"
     gene            3081..3824
                     /locus_tag="PLO70_11450"
     CDS             3081..3824
                     /locus_tag="PLO70_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75126.1"
                     /translation="MKLFAILITIYMFLGFDVLAQKYNGRFSGDQPGLTSSAELTVQN
                     RQLKGKVYMNGKLANVSGTITDSASAGTIFDVEVSKTYSYRSVLRVNELHFFITFPEL
                     NYQELELVMQRENKNPATKPATTRATKSVGNKDARLVGVWRYTEVLSSGSGDNYASFS
                     TDYFMEFKADGTVLSWTGKSAGGSGTVSIDGNGSAKVEKGEWYTEGKTLYLADPVTNQ
                     KVSTLFYAEQNRMMLHNGGSEKKIFQRIQ"
     gene            complement(3845..4657)
                     /locus_tag="PLO70_11455"
     CDS             complement(3845..4657)
                     /locus_tag="PLO70_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQZ75127.1"
                     /translation="MEYLFTSESIISFFILVVLEVVLGIDNVIFVSIILNRLKGDKEQ
                     KKARRFWMVTGILSRSLLLMGLGWLLSQKGKAVFSIAGKGFDLASIVMLAGGLFLIYK
                     SVMEIHHKLEGEDPNELLKNKKPLSFGQAIGQILLIDAVFSFDSIITAGGTAKHVEIM
                     IAAVVIAMIVMFLFSPSISGFIHKHPTLKMLALSFLVMIGLSLIIEGWDSEAAHQLHL
                     KNYIYFGMAFSFTVEMLNMTMRSRAARKRAHLVKLNEPVLENTETNNQGEKL"
     gene            complement(4668..5717)
                     /locus_tag="PLO70_11460"
     CDS             complement(4668..5717)
                     /locus_tag="PLO70_11460"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQZ75128.1"
                     /translation="MYKLLRNILFCFSPEGVHHFSMNALRLLCSVGFSRKILAKIFSP
                     ANPALTTEFFHLRFRNAVGLAAGFDKNAKYLRELECLGFGFVEIGTVTPLPQAGNDKP
                     RVFRLPKDKALINRMGFNNDGVKVVAERLKTWKEKQSRLTTKDYRLIIGGNIGKNKIT
                     PNEEAWKDYEICFKELHPFVDYFVVNVSSPNTPGLRELQEKESLRKILMHLQMINNGK
                     AKAKPLFLKIAPDLTQEQIDDVIDLALEIKLDGLVATNTTISRAGLLTPELRINEIGA
                     GGLSGLPVKQRSTDIIKYITDKTNGAIPIIASGGVFTGIDAKEKLDAGASLVQVWTGF
                     IYEGPAIVKQICKSL"
     gene            5922..7100
                     /locus_tag="PLO70_11465"
     CDS             5922..7100
                     /locus_tag="PLO70_11465"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQZ75129.1"
                     /translation="MMQEAYIIAGYRSAVGKAKRGGFRFTRPDDLAVTVIKGLMASVP
                     QLDAKRVDDVIVGNAVPEAEQGLQFGRIISARALGIDVPGVTVNRYCASGLETIAIAT
                     AKIRMGMADCIVAGGTESMSMVPTAGWKTVPAYSIAKEEPDYYLSMGLTAEAVAKDYN
                     ISREAQDEFSYQSHQKAIAAIKAGHFKPGILPVTVEEIYLDSKGKKQKRNYVVDTDEG
                     PRADTSVEALAKLKPAFAAGGVVTAGNSSQTSDGAAFVIVMGEKMMNELGLKPIGRLV
                     TSAVAGVHPRIMGIGPVAAIPKALKQAGMNLSQIDLIELNEAFASQSLAVIREAGLDP
                     SKVNINGGAIALGHPLGCTGCKLTVQNLHDMKRLNKKYGMITACVGGGQGIAGIIENL
                     "
     gene            7284..8948
                     /locus_tag="PLO70_11470"
     CDS             7284..8948
                     /locus_tag="PLO70_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQZ75130.1"
                     /translation="MDRRDFLTARRQQKQPEPVQLFRTNSGITTYTGQWTEQEIVHLL
                     KRTMFGAKKSDVNYFKTRSMSQAVDELLTPTAPQPAPPVKEYVTSTTPGVNADGNIIQ
                     GTTWINDINSDGTVQSQRRGSYKKWWTGVMINQDRSIREKLIMFWVDHFGNEATEVGN
                     ANWVYKQHDLIRQYALGNFKNLVRDITKDVAMLRYLNGYLNNKNAPDENYARELFELF
                     TLGKGPGSQYTENDVKEAAKVLTGWQINGTTYTSVFNINRHTTTNKIFSSFFNNTVIT
                     GRTDATAGDLELDDLLTMTFAQQEVARFLVRKIYRWFVYYSIDSATETNVIIPLADIF
                     RANNYEIKPVLSALFKSEHFYDMLNRGCLIKTPADQVIGSLREMNVQFRPETEWDTNY
                     GLWNTFYSWMVNMGQNPHDPPNVSGMPAYYQEPLFHEIWINSDSLPKRNQFTDIMINT
                     GYARNGFRVQFNCVAFAQTLSNPGNPNDLIDESLAIFFRNDLSAQSKGQIKTQILLTG
                     QQWDYYWTNAWAAYEANPTTANFNTVNTRLKALFQYFFNLSEFQLA"
     gene            8978..10573
                     /locus_tag="PLO70_11475"
     CDS             8978..10573
                     /locus_tag="PLO70_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQZ75131.1"
                     /translation="MKRRDFLLSTLPAAAILPEVINGYSVKAFNSNSPLVQALLRGTT
                     LTDHVLVIVQLSGGNDGLNMVIPVADYSKYFNARSNVAIPENSVLPIAGVTGTGIHPA
                     MTGLQTLFTEGKAKVIQAVGYPQPNFSHFRATDIWMSGSNSNQEVYSGWAGRYLNNEY
                     PNFPTGYPSTDMPDPLAIQIGSTTSLTLQGPTVNMGMSITNPTNFYNLISGTTDPVPD
                     TPAGKELKFIRQVNQQTQKYSTVIRAAANAVPQQSTYPANNSLADQLKIVARLVKGGL
                     KTRVYMVSFGGFDTHSVQVNAADTTTGTHATLLGRVSAAIKAFQDDLKFLGVEERVVG
                     MTFSEFGRRIKSNSSTGTDHGAAAPMFLFGSPVEAGMLGVNPTIPANATVNDNIPMQY
                     DFRSIYATLLEKWFCLEKTVVDSLFPPDINNQLQSLPLIKAGACSGITPPPPQPTDLT
                     IINYPNPFTSSTTIKFTTEGGHTFIQIIDTLGRVLKVLVDREYTAGTYTTEFNSEGLP
                     TGIYYARFQNGMNQQVRAMMKVR"
     gene            complement(10635..11234)
                     /locus_tag="PLO70_11480"
     CDS             complement(10635..11234)
                     /locus_tag="PLO70_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75132.1"
                     /translation="MTINTRSMRSSSNALFAIVLISTAMLGCSKKDTPIPDPCLGVSY
                     DVQYFKTEAIGSSNNGTIAITFPIGDTISYQLNSGAYQASPGFTNLAPGNYVITVKNQ
                     NGCTDTAQITILNYGPKYALVKQIINGYCGPCHLNGAVNGSKNFDTDASIVSSWDRIK
                     ARAVDGNPSFMPAAPNSPLTNVDKQKITDWVNAGHRQSD"
     gene            11400..>13822
                     /locus_tag="PLO70_11485"
     CDS             11400..>13822
                     /locus_tag="PLO70_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75133.1"
                     /translation="MQKSAILLLAFLWVQVQVNAGDYFSINQSNRIRSTNIKATISGK
                     VVDVKTGEALPGAYVYFIDDKIGTVADAQGKYILTNIPLGHHVIEISHTGYSSLVEHI
                     EINANMERDFALSPVIVENQGVIVTGISGATSIRNVPVPITSIRKSTLLQTPSSNIID
                     ALSHVPGVSQLSTGPAISKPIIRGLGANRVVTINDGVRQEGQQWGDEHGIEIDEMSVA
                     KVEILKGPASLMYGSDALAGVVNFISNVPVAEGTIKGNLLTNYQSNNGLFGANGNIAG
                     NTNGFNWNVYGTYKSAGDYRNKYDGKVLNSRFNERNFGGYAGINKSWGYSHVIFSRFD
                     QRVGLVEGDRDDATGQFLLYAGTPFERIAAKQDLDSRDLFVPQQRVQHTKIVSDNNFA
                     IKNGRLKANIAFQNNQRKEFGNPEDPAEQELFFDLKTINYSLQWQFQEMNEWHTTIGI
                     NGMRQSNQNKGAEVLIPEYNLFDIGGFVFMQRFFKMGTVSGGIRFDNRSVDSKEFFDG
                     PDPKFTAFTRSFSNVSGSIGVSYEPTELVTVKANIARGFRAPSLAELASNGTHEGTNR
                     YEYGVQDLKSETSLQLDGGIDLNYEHFSFGLAAFYNRINDFIFYQKLESVFGGDSLVN
                     VDGEDIMAFVFNQHNAKLSGLELFIDIHPHPLHWLHFKNSFSFVRGRFDNRLDGDRIG
                     SDNIPLMPAPRWNGELRADFKKAGNGFRNLYIRVEADKTFEQDKFFTGYDTETGTNGY
                     LLINAGIGADIVNNKNKALFSLHLGVMNLGDVAWQNHLSRLKYAATNMLTGRTGVFNT
                     GRNFSVKLNV"
BASE COUNT         4095 a         2678 c         3114 g         3935 t
ORIGIN      
        1 tggatacggt acttcatact ttatcctggc tctttgaatc gcttgatcct gtgacacggg
       61 aactggttgc cgatccgaat gacgggtttc tgaacccgaa ccagcaatca ccggaaggtg
      121 agggttttgt ttcgttcagt gttcgccccc tggcgaatcg gcctcacctg cagcaagtga
      181 tgaacacagc caagatcgtt tttgatgaga atgcacccat tttcacaaat gtctggaaaa
      241 atacagtaga catagaaaaa ccatccagcc aggtgttacc tcttcctgct gtgctgaatg
      301 atactacatt ccttgtaaga tggagcggga ccgatgcgca tgcgggaatc atgggttatg
      361 atatctatgc aatcattaac gataccatta cccgtcggat attacgcaat acacctactg
      421 attctatccg gattaacggg gcttttggta atacctacaa attcttttct gtggccagcg
      481 acctcgttgg taatattgaa gatctgccag gtgtgcctga tgctgtgatc accctttccg
      541 caccgctgcc attgaccatt ctttctttta ccggtagccg gcaaaacaat gatgcagtat
      601 tacaatggcg gacagtgaat gaggtaagcg ttagtcattt tgaagtacag cgcagcttca
      661 acggtgccga ttttaccaaa gaaggaactg ttacagctgg tggcactctc tactcgctga
      721 ctgatcccgg tgtttttaac actcagccgg ttgtttatta cagattgaaa tcagtagata
      781 tcgatggacg ttttacctat tctgctatcc tgaaattgag ccgcaatgag aatggattgc
      841 tgacggtctt ccctaacccg gtaaaggatc aactggtgat cggaggtttg aaaaggggag
      901 ctgtgctacg gatatttagc gccgatggaa gactggtgca gcaaacggcg gtaaccactc
      961 aaacgatgat cattgatatg ataggttata cgagcggcat atactggctg caatatgcca
     1021 gcgacggtga aatacaaaca cagaaaattc ttaagcaata atcttgagac atataaccaa
     1081 ataaaaatga ataggaggtt gagatttctc aaataaatcc aggtctttag aatagcacaa
     1141 ttttttttca tgcacagtca gccgggtgtc ctgcaatggc aggcacccgg tttaaatttc
     1201 tttatagtgg aggatttgat ttccggatgt atcgggtatc aaaaattttt attatcgtgt
     1261 aatgctttca atactgctat ccaatgagtt tataatataa ctacctccat tcaggtattt
     1321 ttttcgtgat tttttaaact gattcaggta tatgaaaaat gaggactggc tatgaacttt
     1381 atcttgtaaa atttaaactt tatgcgttgt caattaatgt tattttccgg cctcttattt
     1441 attgctctct ctggttctgc tcagtcagca tgtgatgaat cagcaaatgg gtatactatt
     1501 ccggcaaaag aaataaattg tttcaggcac ttttccagca atcaacaacc cggtgttcat
     1561 gccgttaata attacctgct ggctaagatc agtcagttga tgtataccga gcggctggat
     1621 tttgaattgc gtaagctgcg caacccttca ggctttcccg ccgctgattt tagttccagt
     1681 caactaaatt caagcagtaa taattctttt gagtgtgatt ttaaaaagcg cttcagtcac
     1741 tggttctatg atgtgaacag caaaccggtt cgaccattga aaattgacaa gaacctgcaa
     1801 atggcacccg tagaaaacag gaataaaccc ggggtggtca ataattcaaa tcttaagagt
     1861 aatcaaatgc taaatgataa ggctgattta tccgggaagg cgagggtaat agaaaataaa
     1921 aataccacaa ccatgtcccc ggaacagaag tttatccagg actcagtagc atttgaaaag
     1981 tcaaaaccta agtttaagtt tttgaataaa agaactgatt ttgtcaatat tggcggagaa
     2041 atacgaatac ccggatttga tccggaatta atggttatct ccaccgaaga atacattata
     2101 gttgcgtgga gaggcactga taatgtgtac agggatgatt cctgggaatg gattggaacg
     2161 gatgcatact ttgtaccggt gagtggcgat ggacctttag catctgctaa aatgcatgca
     2221 gggttttggc atagtttcaa agtgatccga aacaagttaa tggctacact ggatgcattt
     2281 gaggcgaaat caaagaataa gaaaatattt ataaccggac atagcctcgg tggtggtatg
     2341 gcaatgatta gtgcgcccta tcttgcggga acaggataca aggtggcaga agtttatacc
     2401 tacgctgccc cccgtgttat cggtgaccag gcttttgtga acagatgtaa taccctttta
     2461 ggcagtgata agatacagcg ttttgaatat ggggtagatt ttgttaccaa attatggtca
     2521 ccggctattt acttctccac ttataaaata cccgggcatc ggcattggct gaatgcacag
     2581 ggtgctgatg atgattataa ttgcggggag agatatttcc ccatgacagc caatccattt
     2641 gagtacaatg attattcccg ggcgaagatt gacaagttga atggcgatgt aggtgctttt
     2701 gatccttttg gatgggcagc attgtttaaa acaagtatta aaggtcaaag ccccaagtat
     2761 ccgaaagagt cacacagtta tcctttgttt gattttggtc agcacaatcc acaatactat
     2821 gtgaagaaag catttgagaa tatgacaaca gagcaaaagg gattgttgcc gggattccag
     2881 gatacttatc catatgttta tcctgctgtg ccgggaaata aataggcttt caggcctggg
     2941 attgatttac aatagaattc agttcaggaa taatagttat tggtcatttc tggaaatgtt
     3001 attcattcta tagtcgtgcc ggaatatttg caatttgaaa taattaaacc aggtaaaagg
     3061 ttacaataaa aaggaaacaa atgaaattat ttgctatact aataaccatt tatatgtttt
     3121 tgggatttga tgtgttagct caaaaataca atggtcgttt ttcaggcgat cagcccgggc
     3181 taacctcttc tgcggaattg acggtccaga acaggcagct caaaggaaag gtttatatga
     3241 atggaaagtt agccaatgtc agcggcacga taacagactc agccagcgcc ggaaccatct
     3301 ttgatgtgga ggtgagtaag acatacagct accggagtgt attgcgggta aatgaattac
     3361 atttctttat cacttttccc gagttaaatt accaggaact ggaattggtg atgcagcggg
     3421 agaataagaa cccggcaaca aagcctgcca caaccagggc aacaaagtca gttgggaaca
     3481 aagatgcaag gttagtaggg gtatggagat atacagaagt actgagcagc ggcagcggtg
     3541 ataattatgc ttctttttct actgattatt ttatggaatt caaagccgat ggtacagtgt
     3601 tatcatggac cgggaaaagt gcagggggta gcggaactgt ttctattgac gggaatggat
     3661 cagcaaaagt tgaaaagggc gaatggtata cggagggaaa aacattgtat ttagctgatc
     3721 ctgttacaaa ccagaaggtg tctactcttt tttatgctga gcaaaacagg atgatgctgc
     3781 ataacggagg tagtgagaag aaaatatttc aacgaataca ataaagcatc ttatcaagct
     3841 attgttatag cttttcccct tggttattgg tttcggtatt ttcaagcacc ggctcattca
     3901 gtttaactag atgggctctt tttctggctg ctcggctgcg catggtcata ttcagcattt
     3961 ctaccgtaaa ggagaaagcc ataccgaaat aaatataatt tttcaggtgc aactgatgtg
     4021 ctgcttcgct atcccagcct tcaatgatca aactcaatcc gatcataaca aggaaggaga
     4081 gggccagcat tttcagtgta ggatgtttat ggataaaccc ggatatagaa gggctaaaca
     4141 ggaacattac gatcatagcg atcaccaccg cagcgatcat gatctccaca tgcttggccg
     4201 tgccacctgc ggtaatgata ctgtcgaaag agaacactgc atcaataagc aggatctgcc
     4261 cgattgcctg tccgaaagaa agcggttttt tattttttaa tagttcattc gggtcttcgc
     4321 cttccagttt atgatgaatt tccatcactg atttatagat caggaataaa ccacctgcca
     4381 gcatcactat actggccagg tcaaatcctt tgccggcaat actgaaaaca gcctttcctt
     4441 tttgcgacag taaccagccc agccccatta gtaacaggct gcgggaaaga ataccggtta
     4501 ccatccagaa ccggcgggcc tttttttgtt ctttatcacc tttcaaccgg ttaagaataa
     4561 tgctgacaaa aattacatta tcaataccca ataccacttc cagcacaaca agaataaaaa
     4621 agctgataat actttctgaa gtaaacaaat attccattcc ttattgttta taatgattta
     4681 caaatttgtt ttacaatggc cggtccttcg taaatgaatc ccgtccatac ctgcaccagc
     4741 gatgcacctg catctaattt ttcttttgca tcaatcccgg taaaaacgcc accggatgca
     4801 ataatcggaa tggctccatt tgttttatcg gtgatatact ttataatgtc tgtacttctt
     4861 tgctttacag gtaagccact taatcctcct gctccaattt catttattcg taactcaggt
     4921 gtaaggagac ctgcgcggct gatagttgta ttggttgcta ccagtccatc taattttatt
     4981 tccagggcca ggtcaatcac atcatcaatt tgttcttgtg taagatctgg tgcaatcttc
     5041 aggaagagtg gtttggcttt tgccttcccg ttattgatca tctgcaggtg cattaaaatt
     5101 ttacgcaatg attctttttc ctgtagctcc cgcaaccctg gtgtattggg tgaacttaca
     5161 tttactacaa agtaatcaac gaatggatgc aattctttaa agcagatctc ataatccttc
     5221 caggcctctt cattaggagt gattttattc ttgccaatat tgcctccaat gattaaccgg
     5281 tagtctttag tggtgagccg ggactgtttt tctttccagg tttttaaccg ttctgcaact
     5341 actttcactc catcattatt aaatcccatc ctgttgataa gcgctttgtc tttcggtaaa
     5401 cgaaataccc ttggtttgtc attaccagcc tgtggtaaag gtgtaaccgt tccgatctcc
     5461 acaaaaccaa aaccgaggca ttcgagttct ctcaggtatt tggcattttt atcaaaaccg
     5521 gcggcgagtc caactgcgtt cctgaatctg agatgaaaga attcagtggt taatgcagga
     5581 ttggcgggtg aaaagatctt agccagtatt ttcctgctaa agccaacact gcacaaaagc
     5641 cggagggcat tcattgaaaa gtgatgcact ccttccggcg aaaaacaaaa aagaatattg
     5701 cgtagaagct tatacattgt ggttgcgaag ataattgaag ttttggggca gttttggtgg
     5761 gtacaacaag tctctttatt tttgatacag aaagttgcat aaacaaccaa atagggtttg
     5821 tagtttgtga tttatagttt gtagttgacc tttttaaagt agaggcagac ttttcaaatt
     5881 ataaagaact acagaccaca aactataaac taaaaaattt tatgatgcag gaagcttaca
     5941 ttattgccgg ttatcgttct gctgttggaa aagccaaacg tggtggtttc cgttttaccc
     6001 gaccggatga cctggctgta acagtcatca aagggctaat ggcatccgtg ccacaattag
     6061 atgcgaaaag agtggacgat gtgattgtgg gtaatgccgt accggaagct gagcaagggc
     6121 ttcagtttgg acgtattata tcagcaaggg ccctgggtat tgatgtgccg ggtgttacgg
     6181 taaaccgtta ttgtgcatcg ggtcttgaaa ccattgcgat tgccactgcc aaaatccgga
     6241 tgggaatggc cgactgcatt gttgccggtg gaactgaaag tatgagcatg gttcccacag
     6301 caggttggaa aacggtgccg gcttattcca ttgccaaaga ggaaccggat tattatttaa
     6361 gcatgggcct tactgctgaa gcagtggcaa aggattataa tatcagcagg gaagcacagg
     6421 atgaattttc ataccagtca caccagaaag caattgctgc aattaaggct ggtcatttca
     6481 aaccaggcat tctgcccgtg acagtggaag aaatttatct tgatagcaaa ggcaaaaagc
     6541 aaaagaggaa ttatgtggta gatacggatg aaggaccaag ggctgacaca tctgtggaag
     6601 ctctggccaa attaaaacca gcttttgctg cgggaggagt agtgacagcg ggcaattcat
     6661 cccaaacatc tgatggtgct gcattcgtca ttgttatggg agagaagatg atgaatgaac
     6721 tgggattgaa acctattgga aggttggtga cttcagctgt ggccggagta catccccgca
     6781 taatgggcat tggccctgtt gctgctatac caaaagcatt aaaacaggca ggtatgaacc
     6841 tgtcccagat tgatcttata gaattgaatg aagcatttgc ttctcaatca ctggctgtga
     6901 tcagggaagc cgggcttgat ccttctaaag taaatatcaa tggtggtgct attgcattgg
     6961 gtcatccgtt aggttgcacc ggttgtaagc tcactgttca aaacctgcat gatatgaagc
     7021 ggctgaataa gaaatacgga atgatcacag cctgtgtggg tggtggacag ggtattgccg
     7081 gtatcattga aaacttataa agccttcttt cttaaaacaa tagagccgtc cggattttcc
     7141 agtcggctct tttttcatac tacgctatgt atggttgttt tactattgca aatgaatagt
     7201 ttggatgcaa tttttgttac ctgctcctta aaccaatttg tatctttaaa gtcgtagttc
     7261 attcctgaac caaaattatt gctatggata gaagagattt cttaacggcc cgcagacaac
     7321 aaaagcaacc cgaaccggtt cagctttttc gtaccaattc cggtattact acttacaccg
     7381 ggcaatggac ggaacaggaa atcgtacacc tgcttaaacg aacaatgttc ggtgccaaaa
     7441 aatccgatgt aaattatttt aagacccgta gcatgagcca ggcggtggat gaattattga
     7501 ctccaacagc accgcaacct gcaccacctg tcaaggaata tgtcacttca acaacacctg
     7561 gcgtaaatgc agatggaaat attatccagg gaactacatg gataaacgat atcaacagtg
     7621 atggtacagt gcaaagccag cgaaggggtt cctataaaaa atggtggact ggtgttatga
     7681 ttaaccagga tagaagcatc agggaaaaac tgatcatgtt ctgggttgac cattttggaa
     7741 atgaagccac cgaagttggt aatgctaact gggtatacaa acaacatgat cttatccgtc
     7801 aatatgcact gggtaacttt aaaaatcttg tacgggatat taccaaagat gtggcaatgc
     7861 tccgctacct caatggttat cttaataaca agaatgcacc ggatgaaaac tatgcaaggg
     7921 agttatttga actttttact ttaggtaaag gccccggctc gcaatacaca gaaaatgatg
     7981 taaaggaagc tgcaaaagtg ctgaccggct ggcagatcaa cggcactacc tatacttctg
     8041 tttttaatat caaccgtcat accactacta ataaaatctt ttcttccttt tttaataata
     8101 ccgttattac tggaagaacc gatgccactg ccggtgatct tgagctggat gatctgttaa
     8161 ccatgacttt tgcacagcag gaagttgcca gattcttagt cagaaaaata tatcgctggt
     8221 ttgtttatta ttctatcgac agtgctacag aaacaaatgt gatcataccg ctggcggata
     8281 ttttcagggc taataattat gagataaaac cggtgctttc cgcattattc aaaagtgaac
     8341 atttttatga tatgctcaac aggggttgcc tgattaaaac tcctgctgac caggtaatag
     8401 gttctttacg ggaaatgaat gtacagttca gaccggagac tgagtgggat acaaattatg
     8461 gcttgtggaa tacgttctac tcctggatgg taaatatggg acaaaacccg catgatccgc
     8521 ccaatgtatc aggtatgcca gcttattacc aggaaccact ctttcatgaa atatggatca
     8581 attctgattc attgcccaaa aggaaccagt ttacagatat tatgatcaat acaggctatg
     8641 cccgaaatgg attccgggta cagttcaatt gtgttgcttt tgcacaaaca ctttctaatc
     8701 ccggtaaccc caatgacctg attgacgaat cgctggctat atttttccga aacgatttat
     8761 cggcccagtc aaagggtcag ataaaaacgc agattctttt gacaggtcag caatgggatt
     8821 attattggac caatgcctgg gcggcttatg aagcaaatcc gacaactgcc aattttaata
     8881 cagttaatac aagactgaaa gcattgttcc agtacttttt taatttatca gaattccagt
     8941 tagcctgaac aatcattttc atccttaatt atagtaaatg aaaagaagag attttttact
     9001 cagcacatta ccagcagcag cgatactgcc cgaagtgata aacgggtatt ctgtaaaggc
     9061 atttaacagt aattcgccgt tggtacaggc tttactacgt ggcactacac ttacggatca
     9121 tgtgttggtg attgtgcagt tgtcgggggg taatgacgga ttgaacatgg tgatacccgt
     9181 tgcagattat tcaaaatatt ttaatgccag gagtaatgtg gccattcctg aaaatagtgt
     9241 gctcccaata gcaggtgtta cgggcacagg tattcatcct gccatgaccg ggttgcaaac
     9301 attatttaca gaggggaaag caaaagtgat acaggcagta gggtatcccc aacctaattt
     9361 ttcccacttc agggctactg atatctggat gagtgggtct aacagtaacc aggaagtata
     9421 cagcggatgg gccgggaggt atttaaataa tgagtatcct aattttccaa ccggctatcc
     9481 tagtacagac atgccggatc cgctggctat tcagatcggc tccaccacat cacttacttt
     9541 gcaaggccct acagtgaata tgggaatgag cataacaaac cctactaatt tttataatct
     9601 tataagcgga acaactgatc ccgtacccga tacaccggca ggaaaagaac ttaaattcat
     9661 ccgccaggtt aatcagcaaa cacaaaagta ttcaactgtt atccgtgctg cagctaatgc
     9721 agtgccgcaa caaagcactt atccagccaa caattccctg gctgaccagc taaagatcgt
     9781 cgccaggttg gtcaaaggtg gtttaaagac aagggtatat atggtgagct tcggtggttt
     9841 tgatacacac tctgtacagg ttaatgcagc tgataccacc acaggtacac atgctacttt
     9901 gttagggcgg gtatctgccg ctatcaaagc attccaggat gatttgaaat tcttaggcgt
     9961 agaggaaagg gtggtaggta tgacattcag tgaatttggc agaagaataa aatccaactc
    10021 aagtacgggt actgatcatg gtgctgcagc ccctatgttt ttattcggca gcccggtaga
    10081 ggccggtatg ttgggtgtaa accccactat acctgccaat gcaacagtga atgataatat
    10141 accaatgcag tatgatttca gaagtatata tgccacctta ttggaaaaat ggttttgcct
    10201 ggaaaaaacg gtagtggatt cattgttccc cccggatatc aataatcaat tacaaagtct
    10261 gccattgata aaggccggtg cttgttccgg aatcactccg ccaccaccac agccaaccga
    10321 tcttactatt atcaactacc cgaatccttt taccagcagt acaactatta agtttaccac
    10381 ggagggtggt catacattta tccagatcat cgatacattg ggtcgtgtgc tgaaagtttt
    10441 agtggacagg gaatatactg cgggtactta tacaactgaa ttcaatagtg aaggattacc
    10501 aacaggtatc tattatgccc gttttcagaa tgggatgaac cagcaggtaa gagcaatgat
    10561 gaaagtgaga taagaagtaa actgaaaaag caatgcccgg taatttattt aaccgggctt
    10621 ttttgtggat attgttaatc actctgccgg tgccctgcat tcacccaatc agtaattttc
    10681 tgcttatcaa cattcgtcaa cggactattg ggcgcagcag gcataaagga tggattgcca
    10741 tcaacagccc ttgctttaat tctgtcccaa ctgcttacta tacttgcatc tgtatcaaaa
    10801 ttcttactgc cattcacagc tccattcaaa tgacaggggc cacaataccc attgatgatt
    10861 tgttttacca atgcatactt aggtccataa ttcagtatgg taatttgtgc ggtatctgta
    10921 catccgttct gattcttaac ggtgataaca taattgccgg gcgccaggtt ggtaaaaccg
    10981 ggtgaagcct ggtaggcacc gctgtttagc tggtaactga tggtatcgcc tatgggaaaa
    11041 gttatagcga ttgtgccgtt attggaacta ccgattgcct ctgttttaaa atattgtaca
    11101 tcataactaa cacccaggca gggatcggga atgggagtgt cttttttgct gcagcccagc
    11161 attgcggttg agatcaatac tattgcaaag agtgcattgg aggatgaacg catggatcgg
    11221 gtgttgatag tcattaaatg taaaaatttt atccggcata aagaagaatt ttaggccatg
    11281 aaccgggttt ataggacttt aagttcgttt taaagattag tgggcccggg cttataaaat
    11341 taaatttgta tgaaacgatg ttgcatttta tatttgcaac attgttacaa taaaatagta
    11401 tgcaaaaatc agcaatcctc ttgttggcat tcctgtgggt acaggtacag gtaaatgccg
    11461 gtgattactt ttcaatcaat caaagtaatc gtatacgtag tacaaatata aaagccacta
    11521 taagcggaaa agtagtagat gtaaaaacag gcgaagcatt accaggggct tatgtatatt
    11581 ttatagatga taagattgga acggtagctg atgcacaggg aaagtatata ctgactaaca
    11641 ttccgcttgg tcaccatgta atagaaattt cccataccgg ttacagtagt ttagttgagc
    11701 atattgagat aaatgccaat atggagaggg attttgcttt atctcctgta atcgtagaga
    11761 accagggggt gatagttaca ggtatttccg gcgctaccag tataagaaat gtacccgtac
    11821 ccattacttc gatccgaaaa tcaactttac tacaaacacc ttcttccaac atcattgatg
    11881 cactgagcca tgtacccgga gtttcgcaat tgtcaaccgg acctgccatt tccaagccta
    11941 ttatccgtgg ccttggggct aaccgggtgg ttactatcaa tgatggagta agacaggaag
    12001 gacagcagtg gggtgatgaa cacggaattg aaatagatga gatgagtgtg gcaaaggttg
    12061 aaatattaaa aggtcctgca tcattaatgt atggcagtga tgcattggcc ggtgttgtga
    12121 actttatttc caatgttcct gttgcggaag ggactataaa aggtaacctg ttaaccaatt
    12181 atcaatcgaa taatggttta tttggagcca atggtaatat agccgggaat acaaatggat
    12241 ttaactggaa tgtgtatggg acttataaat cagccggtga ttaccggaat aaatatgatg
    12301 gcaaagtact caactcaagg tttaatgaaa ggaacttcgg agggtatgca ggaataaata
    12361 aaagctgggg ctatagtcat gttattttca gccggtttga tcagcgggtt ggattagtgg
    12421 aaggggacag ggatgatgcc accggtcagt ttcttttata tgccggtact ccctttgaac
    12481 gaattgctgc aaagcaggac cttgattccc gggatctgtt tgttccgcaa caaagagtgc
    12541 agcatacgaa aattgtaagt gataacaatt tcgccatcaa aaatggccgt ttaaaagcaa
    12601 atattgcttt tcagaataac cagcgtaagg aatttggcaa cccggaagat cctgccgaac
    12661 aagaattatt ctttgatctg aaaacgatca actacagctt acaatggcaa tttcaggaaa
    12721 tgaatgaatg gcacactacc attggtataa atggaatgcg tcaatcaaac cagaataaag
    12781 gtgcagaagt actgataccg gaatataatt tgtttgatat cggaggattt gtttttatgc
    12841 aacggttttt caaaatgggg acagtaagcg gaggtatcag gtttgacaac cgttccgtcg
    12901 attcgaagga attttttgat gggccggatc caaagtttac tgcgtttaca agaagttttt
    12961 caaatgtatc gggtagtatc ggagtcagtt atgagccaac agagttagta actgtaaaag
    13021 ctaatatcgc aagagggttc cgggccccgt cactggcaga gttagccagt aatggtaccc
    13081 atgaaggaac caaccggtat gagtatgggg tgcaggatct gaaatcggaa accagtctgc
    13141 aattagatgg tggtatagac cttaattacg aacatttcag tttcggcctg gctgctttct
    13201 ataaccggat caatgatttt attttctatc agaaactgga atctgttttt gggggagatt
    13261 cattggtaaa tgtggatgga gaggatatta tggcatttgt atttaatcag cataatgcta
    13321 aactctccgg gcttgaatta ttcattgata tccatccgca tccattgcac tggttgcatt
    13381 ttaaaaacag tttttctttt gtaagaggtc gttttgataa ccggttggac ggggatcgca
    13441 ttggcagcga caatatcccg cttatgccgg caccaagatg gaatggggag ttaagggcag
    13501 attttaaaaa ggcgggtaat ggtttcagga acctgtatat aagagtggaa gccgataaga
    13561 cttttgagca ggataagttt tttaccggtt atgatacaga aacaggaact aatggctatc
    13621 tattgatcaa tgcaggaatc ggggctgata ttgtcaacaa taaaaataaa gcactgttca
    13681 gtttgcattt aggagtaatg aatttaggtg atgtggcctg gcaaaatcac ctgagccgct
    13741 taaaatacgc tgcaaccaat atgttgacag gaagaaccgg tgtcttcaat accggtcgta
    13801 atttttctgt caaactaaat gt
//
LOCUS       DAORTI010000091        13778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4323_length_13778_cov_7.533630, whole genome shotgun sequence.
ACCESSION   DAORTI010000091 DAORTI010000000
VERSION     DAORTI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4323_length_13778_cov_7.533630"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLO70_11490"
     CDS             complement(<1..509)
                     /locus_tag="PLO70_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HQZ75134.1"
                     /translation="MELSQDTKNILGLQLPTDPRWVNLAEKSLEEILTDHAYCEQKAA
                     TTCISLIQRYSSKEKLVAELSPIVTEEWGHFRLVLAELQKRNLKLGKQRKDEYANALF
                     DFQQKGGDAEGRMLDQLLTMALIEARSCERFKRLSEGLNDGYMQKFYRRFMESEAGHY
                     TLFIELAET"
     gene            667..936
                     /gene="rpsO"
                     /locus_tag="PLO70_11495"
     CDS             667..936
                     /gene="rpsO"
                     /locus_tag="PLO70_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQZ75135.1"
                     /translation="MPLTKEKKASVLKTYAGSEKNTGSIEGQIALLTERISQISGHLQ
                     QNKKDFSTHRGLMKLVGERKSLLNYLQKHNLTGYRQLIEKLGLRK"
     gene            1214..3403
                     /gene="pnp"
                     /locus_tag="PLO70_11500"
     CDS             1214..3403
                     /gene="pnp"
                     /locus_tag="PLO70_11500"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQZ75136.1"
                     /translation="MLQQPIRKTFDIGGGRMVTIETGRLARQADGAVTVSMGNCMILA
                     TVVANREPKEGQSFFPLSVDYQEKFAAAGRIPGSFFKREARLNDYEILISRLIDRALR
                     PLFPEDYLCEVQVLLSLISSDPEIMPDAMACLAASAALAVSDIPIKEIISEVRVSRIN
                     GEFVVNPTRSQMEKSDMDFIIAATEKNLMMVEGEAQECSEADLCKALEIAHDAIRIQV
                     KAQEELRDLVGVKGKREYSKPATDESLKEKVYAFAKQKVYEVAKGKFSKHERSDKFDA
                     IKVELVDFLKKDLNLGEGESLPDLTKKLTSTYYSDLQYDVIRDMILNEKVRLDGRDLE
                     TVRPLDMEIDVLPSPHGAALFTRGETQSLTTVTLGTPLDELLVESAAKSVDSKFFLHY
                     NFPPFSTGEVKMMRGQSRREVGHGNLAQRSLKQMMPTGDFPYTVRVVSDILESNGSSS
                     MATVCAGSLALMDAGIPVPKHVSGVAMGLITKDDKYAILTDILGDEDHLGDMDFKVTG
                     TKDGICGVQMDIKVDGLKMEVMQQALDQAKRGRMHILDAMYKCIPEARSDVKPHSPRM
                     VKLFIDKEFIGAVIGPGGKVIQEIQRETGTTVNLEEVNNRGEVSIFSTNKEAVDKAVS
                     WIKGIVAVPQVGDVYEATVKSIMPFGAFVEFLPGKQGLVHISEVSWKRLETLEGLVHE
                     GDVMKVKLIGTDPKSGKFKLSRKILMPKPEGQGQPRQEQTPPQEGAN"
     gene            3444..4256
                     /gene="prmA"
                     /locus_tag="PLO70_11505"
     CDS             3444..4256
                     /gene="prmA"
                     /locus_tag="PLO70_11505"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQZ75137.1"
                     /translation="MEYIQIDFQNITSEQTDLLIAELTMIGFEGFEEGENSLKAFIPL
                     SDFIEAHLKEVTDKNKCSFTLSVIKETNWNAVWESNFQPVIVDDFAGIRADFHEPMKE
                     VEHEIIITPKMSFGTGHHATTFLMLQQMRKIDFKDKTVFDFGTGTGVLAIMAEKLGAA
                     GITAVDNDKWSIENATENIERNKCNSIRVIKADTALLNEQFDIILANINKNVILDNLS
                     ILKEQLKPNGVLLLSGLLSNDQNDIIESATNLSLQLIDKQEKNNWISLRLLH"
     gene            4359..5027
                     /gene="plsY"
                     /locus_tag="PLO70_11510"
     CDS             4359..5027
                     /gene="plsY"
                     /locus_tag="PLO70_11510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQZ75138.1"
                     /translation="MNELLLIILAYLIGSVPTSVWVSKSFFKIDIRDYGSGNAGATNT
                     YRVLGPKWGTFVMIIDMLKGVLAVKLALLLPEYADSEVNLQNLQTGLGLAAVIGHIFP
                     IWADFRGGKGVATLFGLVLGISPWTALSCVGIFSLVLYLTRFVSLSSILASIAFPIFI
                     LVIFNVENPAYRVFAIAVALMVLLTHQKNIGRLLRGSESKVPILKNRDRKRQRRNEAS
                     QNND"
     gene            5120..5932
                     /locus_tag="PLO70_11515"
     CDS             5120..5932
                     /locus_tag="PLO70_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQZ75139.1"
                     /translation="MIRKIIAFLVLTVLIIACSKNALTGKKQLTLLPETELQSMASTQ
                     YMQFLSSNKVVSTSNNRDAEMVKRVGDRIVKSVENFYADKGMADKLAGFKWETNLVDD
                     KTVNAWCMPGGKIVVYTGILPITQNEAALAAVMGHEVSHALLQHGNQRMSQGLLQQLG
                     GVALSVAVANKPQETQNLFLGAYGAGSQLGILLPFSRKHELEADRYGLIFTAMAGYNP
                     QEAIGLWERMEKASNGQKPPEFLSTHPSEGHRIEQLKKYMPEALQYYKPLGK"
     gene            6109..6555
                     /locus_tag="PLO70_11520"
     CDS             6109..6555
                     /locus_tag="PLO70_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75140.1"
                     /translation="MSQTVFEKLQLADEKNLLIQGLPSSIEKQFSKLSFCKNMTPLLK
                     TRKIDFALVFAVSENQLNGILKDVMPSLKDGSKFWVAYPKTTSKIVTDLNRECSWNHL
                     TCEGYESIERVELDHVWSAMRFTKGENNRGEEVMSTRKRQAVAVAE"
     gene            6615..7145
                     /locus_tag="PLO70_11525"
     CDS             6615..7145
                     /locus_tag="PLO70_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HQZ75141.1"
                     /translation="MTFLNVEIKAKCRNSNRVREYLHNNGAEYRGTDEQTDTYFNVPN
                     GRLKLREGNIENNLIFYDRGNQAGPKDSHFHLVKVEDANGLKEVLTKSNKIKIIVRKR
                     REIYYLANVKFHIDEVPELGSFIEIEAGNILADLSALQLREQCNFYLHELGIENEDLV
                     EVSYSDMLLSSNGSNG"
     gene            complement(7135..9168)
                     /gene="dnaG"
                     /locus_tag="PLO70_11530"
     CDS             complement(7135..9168)
                     /gene="dnaG"
                     /locus_tag="PLO70_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQZ75142.1"
                     /translation="MISPNTIQQILGRIDIIDVVGSFVKLKKRGTNYLGLCPFHNEKT
                     PSFTVSPSKEIYKCFGCGKSGNTVSFIMEHEKYSYVDALKWLANRYGIEIEETFVNDE
                     QRQQMLAADSLYIINGFAQQFFTRMLFETEEGQDIGLAYFKERGFREDIIKKFQLGYS
                     PEQRDAFTKEAIAKQYNTELLIKSGLVANRNEQLVDNYRGRVIFPIHNHSGKVLGFGA
                     RILKSNDKAPKYINTPENEIYIKSKILYGSYFARQAIDKADECLLVEGYTDVISLHQA
                     GIENVVASGGTSLTPDQLRLVKKYTNNLTIVYDGDAAGVKAALRGLDLALEEGLNVKL
                     VLIPDKEDPDSYVNKVGAAAFIQFVQKNKKDFILFQLEVALKDAGTDSVKKSEVVNRM
                     AETISRINKAEDFTRQQDYIKQCSEILKIDESGLHSLVNKFIRDRISTLERKLPFEEA
                     KQFEQNAKQAEQTGFDEATFNLLFKDELQEREVARILLEYGIKKWDDKKLIAEHIIEE
                     MVDESLLDNSDVIRLIAVFKELLQQNPQAANRSYFIHHSDTKLSAFAVSLLNFPYEES
                     EHWRSEFSQASGYQKSLFEQSYEDFIRTVAPENKEQLMSFLKIDEDKTNEGVDSAINY
                     LKLRKIKRMLLENQQDLEKEHTTEEFHMLHQTHEHLKQMEMELTKKIGTVIIR"
     gene            9286..10863
                     /locus_tag="PLO70_11535"
     CDS             9286..10863
                     /locus_tag="PLO70_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQZ75143.1"
                     /translation="MNKILTLPFILITLIASANNVQLTNISVTNNGTNTGKIIEFDLT
                     WENSWRTASTGNWDGVWVFFKFKDNDGTWYPLHFTGTDIIMPAGAAYEMGNSGVVTGV
                     GMFIYRAANGFGTAVATGIKAGIESFPGTFEIRGFALEMVFVPGGSFWLGDETSVNSY
                     KEGSTANSPFQVTANGAGTNMGSSTGLLYDPQSAYSGNIPGFPTGYSGFWMMKYELSQ
                     GGYRDYFNTLTYTQQLNRLRDIFSNPASPTGTSINNSAACCRQYMEIAIPGNSTTNTP
                     AVIGLDADGDNIFNEATDGEWITATYFTWPDVASYLDWAGLRPMTELEFEKACRGPLT
                     PVAGEFAWGTNTIASYPYTIANAGTATENISNMSAILGNCNSGTSLVSLLRGGIFATG
                     VSTRVSAGAGYYGTMELSGNISEIAITTGNEAGRSFNGKLGDGLLTTAGNANENNWPG
                     VNGNTGTNVAPGNYDGGLGVRSDGGIRWRGGGFTQINNDSYKVSDRILFSGNGTDIFT
                     NQTNKSFVGIRGVRDAN"
     gene            10870..11460
                     /locus_tag="PLO70_11540"
     CDS             10870..11460
                     /locus_tag="PLO70_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75144.1"
                     /translation="MQKILFKIWITLITTIPLSMHAQFPYSGGNGNGASGVSSVNISL
                     SITDSLYNGGNGDGVTKNVLLNAVLSQADSMYKGGQGNGFHSIVLTNTTLNLLDSIYN
                     GGTGRGDNQKVAPAINLSICTDVLVWNGNANINWDNPGNWDCGTVPGVNSIVIIPGGR
                     PRYPTIFTNTVIKKLEMQPGASVTVFTNRLLLINGQ"
     gene            11740..12165
                     /locus_tag="PLO70_11545"
     CDS             11740..12165
                     /locus_tag="PLO70_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75145.1"
                     /translation="MDAAKVFVDVIIPGKQGSNKAMNDLIDDATNMQIDLYKSYGVIA
                     GQSNATIGAVDLIVPLKKWTGELYTERTFQIMQSPYDKIVITIKKTDGKRGVEFTACA
                     KYSNGSPYDTKSGKIENGNETAGQVRTITFPRNMLDKNI"
     gene            12175..12495
                     /locus_tag="PLO70_11550"
     CDS             12175..12495
                     /locus_tag="PLO70_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75146.1"
                     /translation="MAHGALPTDKCEYTLTVEGFFDENEMKEIAKTNPAEKDNKKSQL
                     KESNTSPAKVSSGNQPAKMNSTKQPGVTNEIVSPRDPASGLPAGKKVQTAPDSLSQKK
                     KKNN"
     gene            12687..13598
                     /locus_tag="PLO70_11555"
     CDS             12687..13598
                     /locus_tag="PLO70_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75147.1"
                     /translation="MKTFKYVYSAFFFTCIMFVNVTAQNQPGVDSTGLPGDDFSLQGA
                     LQMFQDATTIEDFEKAINTEGYHINNLDLNADGEIDYIRVVDKSEKDVHAFVLQVPVS
                     ETENQDIAVIELEKTGEANAIIQIIGDEDIYGEQVIVEPDGGGEEAFYNFNDSKLSGP
                     NAGYDFTTPGIVVNVWLWPSVRFVYGPAYRPWISPWRWRYYPGWWKPWKPLRWHAWHP
                     FRAHYHRPFSVVRTHRVIHAHRVYTPFRTTSVTVRTRNSAAVGNFRVTRTKTSITGPR
                     RKTTTVKKTTVTGPRGGKATKVKARRH"
BASE COUNT         4204 a         2564 c         2987 g         4023 t
ORIGIN      
        1 tatgtttcag ccagttcaat aaatagtgta taatgcccgg cttcactttc cataaaacgc
       61 cggtaaaatt tttgcatata cccgtcattc aatccctcgc ttaatcgttt gaatcgttca
      121 caactgcggg cctcgatcag tgccattgta agcaactggt caagcatacg gccttctgca
      181 tcgccccctt tctgttgaaa atcaaacaat gcattagcgt attcatcttt tctttgttta
      241 cctaatttca ggtttctttt ttgtaattct gcgagtacca gccgaaaatg cccccattct
      301 tcggtaacaa tgggagagag ttcggctacc agcttttctt tggagctgta tcgttgaatc
      361 agtgaaatgc aggttgtagc ggctttttgt tcgcaatatg cgtggtcagt tagaatttct
      421 tctaatgatt tttcagccag attgacccat cgcggatcag ttgggagctg gaggccaagt
      481 atatttttgg tgtcctggga gagttccata gcgtaaaaat aagttatgcc ctgaaaatca
      541 gcataaatgg gatgtaaata cctcaaaaaa agggtggcaa accctttgct gttaacgtta
      601 tccgccttac ctttgccgcc ttaataacaa gatgaaaaaa acggatgaaa tttaaaaatt
      661 aaaaagatgc ctcttacaaa agaaaaaaaa gcttcagttt taaagactta cgccggttct
      721 gaaaaaaaca ccggttcgat cgaaggtcaa attgcactgc tgactgaacg tatcagtcag
      781 atcagtggtc acctgcaaca aaataaaaaa gacttctcta ctcaccgtgg actgatgaaa
      841 ttagtgggtg aacgcaaaag tctgctgaac tatttgcaga agcataacct gactggttat
      901 cgccagctga ttgagaagct cggattaaga aaataagcag ttcctgaatc cgcccagtgg
      961 cggacgttgg gagagtcttt aatcctgggt tttcgtataa agaaaccaag attttatata
     1021 agctattccc aatgtcttcc ggatgttggg aataactgtt tttgggctgt cagggatttc
     1081 gatgctttca tatgtttcgt aaagccaata gcgacaaaaa cacttaaaaa aaggcacagg
     1141 aattcaagtg attaccagta gctctcacaa aaatcttcac tgggtttaat aaaccctaaa
     1201 atatttagaa cgtatgttac aacaaccgat tcgtaaaaca tttgatatag gtggtggccg
     1261 tatggttact atcgaaaccg gccgccttgc ccgccaggcc gatggagccg taacggtttc
     1321 catgggcaat tgtatgatcc tcgcaactgt ggttgctaac agagaaccaa aagaaggaca
     1381 gagttttttc ccactctccg ttgactacca ggaaaaattt gcagctgccg ggcgaattcc
     1441 ggggtcattc tttaaaagag aagcaaggct gaacgattat gaaatattga tcagccgatt
     1501 gatcgacagg gcacttcgtc ctttatttcc tgaagattat ctctgcgaag tgcaggtgct
     1561 tttgtcactt atctccagtg atccggaaat tatgcccgac gccatggctt gccttgcagc
     1621 ttctgccgca ttagctgttt ctgatattcc tattaaagaa attatatcgg aagtaagggt
     1681 ttccaggata aatggcgaat tcgttgtaaa tcctacccgc agccagatgg agaaatctga
     1741 tatggacttc atcattgccg caaccgaaaa gaacctgatg atggttgaag gtgaggcaca
     1801 ggaatgcagt gaagctgacc tttgtaaagc attggaaatt gcacatgatg caatccgcat
     1861 acaagtgaag gcacaggagg aattaagaga tttggttggt gtgaaaggga aaagagaata
     1921 ttcaaaacct gctactgatg agtcactgaa agaaaaagtg tacgcttttg ccaaacagaa
     1981 agtttatgaa gtggcaaaag gtaaattcag taagcatgaa cgcagtgata aatttgacgc
     2041 tattaaggtt gaactggttg attttttgaa aaaagatctg aacctgggtg aaggtgaaag
     2101 tttgcctgat ctgacgaaga aactgacctc tacttactat agcgatctgc agtatgatgt
     2161 gatcagggat atgattttga atgagaaagt acgactggat ggaagagacc ttgaaacagt
     2221 tcgtcctctt gatatggaga tagatgtatt gccttctccg cacggagctg cattgtttac
     2281 caggggtgaa acacaatcac ttacaactgt taccttaggt actccgttag atgaactttt
     2341 ggtggaaagt gctgctaaat ctgtggattc gaaattcttt ttacattata atttcccgcc
     2401 attctctacc ggtgaagtaa aaatgatgag aggacaaagc cgccgggaag ttggacatgg
     2461 taacctggca cagcgttcgt taaagcaaat gatgccaaca ggtgactttc catatacggt
     2521 tcgtgtcgta agtgatatcc ttgagtctaa tggttcctct tcaatggcca ccgtttgtgc
     2581 tggttcattg gcgctgatgg atgctggtat tcctgtacct aaacatgtaa gcggcgtggc
     2641 aatgggattg attaccaaag acgacaaata tgctatcctg acagatatac tgggtgatga
     2701 agaccatctc ggtgatatgg actttaaggt gaccggtact aaagacggta tttgtggagt
     2761 acaaatggac atcaaagtgg acggattgaa gatggaagtg atgcagcagg cactggacca
     2821 ggctaagcgt ggccgtatgc atatccttga tgccatgtat aaatgcattc cggaggccag
     2881 atccgatgtg aaaccacact ctcctagaat ggtgaaactg tttattgata aagaatttat
     2941 cggtgctgtt atcgggccag gtggtaaagt cattcaggaa attcaacgtg aaacaggcac
     3001 tacggtaaac ctggaagaag taaataacag gggagaagtg agtattttca gtacgaataa
     3061 agaagccgtt gataaagctg tttcctggat caaagggatt gtagcggtac cacaggtcgg
     3121 cgatgtatat gaggcaactg taaaatcaat catgcctttt ggtgcatttg ttgagttcct
     3181 tcctggtaag cagggattgg tacatatcag cgaagtgagc tggaaacgtt tggaaacact
     3241 ggaaggatta gttcatgagg gagatgtgat gaaagtaaag ttgattggta cagatcctaa
     3301 atccggtaaa ttcaaattgt cgagaaagat tttgatgccg aaacctgaag gccaggggca
     3361 acccagacaa gaacaaacgc caccacaaga aggcgctaat taataataaa accctgagaa
     3421 atcagggttt tttaatagtg gctatggaat acatacaaat tgactttcaa aatatcacat
     3481 ccgaacaaac ggatttgttg attgcagaat tgacaatgat cggttttgaa ggttttgaag
     3541 aaggagaaaa tagcctaaag gcctttatac cgcttagtga ttttattgaa gctcatttaa
     3601 aggaagtaac cgacaaaaat aaatgttcat ttactctatc ggtaattaaa gaaactaatt
     3661 ggaatgctgt atgggaatca aattttcagc cagtgattgt tgatgatttt gcgggtatca
     3721 gggcagattt tcatgaacca atgaaagagg ttgaacatga gattatcatt actcccaaaa
     3781 tgagttttgg aactggtcat catgcaacta cctttttgat gttgcaacag atgagaaaaa
     3841 ttgattttaa ggataaaacg gtttttgatt ttgggaccgg cacaggagta ctggctatta
     3901 tggcagaaaa actgggtgca gctggaatta ctgctgttga taacgacaaa tggagtattg
     3961 aaaatgcaac tgaaaacata gaaagaaata agtgtaattc tatcagggtg attaaagctg
     4021 ataccgcctt attaaatgag caatttgata taatactggc caatatcaat aagaatgtaa
     4081 ttctggataa tctttcaata cttaaggaac aattaaagcc aaacggagta ttattactta
     4141 gtggtttact gagtaatgat caaaatgata ttattgagtc ggctactaac ctgtccttgc
     4201 aacttattga taaacaagaa aaaaataact ggatcagttt acgattgctc cattaattca
     4261 tctttatctg tgggttaatt taatattacc acttcctttt tttgcttatt tttgtatctc
     4321 ggtttggtga aaagcaaatc gtgtaactac aacgttctat gaacgaactt cttctgatca
     4381 tccttgcata tctgattggt agtgtgccca cttctgtatg ggtgagcaag tcttttttca
     4441 aaattgacat tcgtgactat ggtagtggga atgctggcgc cactaatacg tatcgtgtcc
     4501 tgggacccaa atggggtact tttgtcatga ttattgacat gttaaagggg gttttagcag
     4561 ttaaactggc acttttatta cctgaatatg ctgatagtga agtgaacctt caaaacctgc
     4621 aaactggttt ggggttagca gccgttattg gacacatctt tccaatctgg gctgattttc
     4681 ggggtggcaa gggagtagca accttgtttg gcctggtcct ggggatttct ccctggacag
     4741 ctttaagttg tgttggcatt ttttctttag tgctttatct tacacgattt gtttccctga
     4801 gttcaatttt agccagcatt gcattcccta ttttcatatt ggttattttc aatgttgaaa
     4861 atccggctta tcgtgtgttt gccattgcgg tagctctgat ggtcttgtta acccaccaga
     4921 agaatatcgg ccgtctgcta agaggaagcg aaagcaaagt accaatttta aaaaaccggg
     4981 atcgtaagcg gcaacgccgt aatgaagctt cacagaacaa cgactaagtc ggtaaaaaaa
     5041 tctaaattta aagtatcaaa tatttgtcat tgtgccaacc gggtataatg tttaatcgtc
     5101 atttctaaaa tttaccaata tgatacggaa aataatcgct tttttggttt tgactgtact
     5161 gattatcgcc tgttccaaaa acgctttgac aggtaaaaag caactgacct tattgcctga
     5221 aacagaactg cagtctatgg cctcaacgca atacatgcaa ttcctatctt ccaataaagt
     5281 agtttcaacc agtaataata gagatgcgga gatggtaaag agagtaggag atcgtattgt
     5341 taagtcagtt gaaaactttt atgcagataa agggatggct gataaattag caggctttaa
     5401 atgggaaaca aatcttgtag atgataaaac ggttaatgcc tggtgtatgc cgggaggtaa
     5461 aattgttgtt tatacaggta ttctgcccat tacccaaaat gaagctgcct tagcggcagt
     5521 aatgggccat gaagtatctc atgccttact gcagcacggt aatcaacgga tgagccaggg
     5581 gttgttgcaa caattgggtg gagttgcctt atctgttgca gtggccaata aaccacagga
     5641 aactcaaaat ctttttttag gagcctacgg agctggttcg caactgggaa tattactacc
     5701 tttttccagg aaacatgaac tggaagcaga ccggtacggt ttaattttta cagcaatggc
     5761 tggttataat ccacaggaag caattggttt atgggaaaga atggagaaag catcaaatgg
     5821 acagaaacca cctgaatttt taagtacgca cccatccgaa ggtcatcgaa tagagcaatt
     5881 aaagaaatat atgcccgagg ctttgcaata ttataagccg cttggcaaat aatttaattg
     5941 attttataca agaatcccgt tcaaaacagc tttgaacggg atttttcttt gatcagggct
     6001 taataatacg ccgtattatt agtacctttg cactccctgc tttgagaact cggtcggcta
     6061 tttatcacgc tggaaagctg actggaacct ttaaaatagc ttctaagtat gtcacaaact
     6121 gtttttgaaa agttacaact ggcagacgaa aagaatcttc taattcaggg tttgccctct
     6181 tctattgaga aacaattcag caaattatcc ttctgcaaaa acatgacccc gttattaaaa
     6241 acacggaaaa ttgattttgc tctggtattc gccgtaagcg aaaatcagct gaatggtatt
     6301 cttaaagatg ttatgccctc attaaaagac ggttcaaaat tctgggtcgc ctatcctaaa
     6361 acgacgtcaa aaatcgttac ggatcttaac cgtgaatgca gctggaacca cctgacttgt
     6421 gaaggttatg aaagtatcga aagggtggaa cttgatcatg tatggagcgc tatgcgattc
     6481 actaaaggcg aaaataaccg tggtgaagag gttatgtcaa cccgtaagag gcaagctgtt
     6541 gcagttgcag aataattttc cggatcttaa aatgtattta ggtttgtcgt aattacgaca
     6601 aacctttttt aattatgact tttcttaatg tagaaataaa agcaaagtgc aggaatagta
     6661 acagggttcg tgaatacctg cacaacaatg gtgctgagta caggggtact gatgagcaaa
     6721 cagacaccta ttttaatgta ccaaatggaa ggctgaaact acgggaagga aatattgaga
     6781 ataacctcat tttttatgac cggggcaacc aggccggccc taaggattct cattttcacc
     6841 tggtaaaagt tgaagatgcg aatggcttga aagaggtatt gactaaatca aacaaaataa
     6901 aaatcattgt tagaaaaaga agggaaattt attatctcgc taatgtaaag ttccacattg
     6961 atgaagttcc tgaattaggc tcatttatag aaattgaagc tggtaacata ctggctgatc
     7021 tgtctgcgct acagttaaga gagcaatgta atttttacct gcatgaatta ggaattgaaa
     7081 atgaggacct tgttgaagta tcatacagcg atatgttact atcatcaaat gggtctaacg
     7141 gataattaca gtaccaatct tctttgttaa ttccatttcc atttgtttga ggtgttcatg
     7201 agtctggtga agcatatgaa attcttcagt ggtgtgttct ttttccaagt cttgctgatt
     7261 ttccaggagc attcttttta ttttccggag tttgagataa tttatagctg agtcaacccc
     7321 ttcattggtt ttatcttcat ctatttttaa gaagctcatc aattgctctt tattttcagg
     7381 tgccacagtt cttataaagt cttcatagga ttgctcaaaa aggcttttct gataaccgct
     7441 tgcctggcta aattcactcc gccaatgctc actttcttcg tatggaaaat tcagtaaaga
     7501 aacagcgaaa gcactgagtt ttgtgtcgga atgatgaata aaataactcc tgtttgctgc
     7561 ctggggattt tgctgcaata actctttgaa aacagcaata agcctgatga catctgaatt
     7621 gtccagcaaa ctttcgtcta ccatttcttc gataatatgt tctgctataa gctttttatc
     7681 atcccatttt ttaataccat actccagtaa tatcctggct acttcccgtt cctgtaattc
     7741 atctttaaat aataaattga atgtagcttc atcaaaacct gtttgttctg cctgttttgc
     7801 attttgctca aactgttttg cttcttcaaa aggtaatttc ctctctaagg ttgagatacg
     7861 gtcacggatg aacttattaa cgagggagtg caaaccactt tcatcaattt tcagtatttc
     7921 agaacattgc ttgatataat cctgttgcct ggtaaaatct tcagctttat taatacggga
     7981 aatggtttcc gccatacggt ttaccacttc tgattttttt actgaatcag tacctgcatc
     8041 ttttaaagcg acttcaagct gaaaaagaat gaagtctttt ttatttttct gaacaaactg
     8101 gataaatgca gccgctccaa ctttatttac ataactatcg ggatcttctt tatcaggtat
     8161 taataccagt tttacattca atccttcttc caatgcaagg tcaagtcccc gaagtgcagc
     8221 cttcacaccg gccgcatcac cgtcatatac aatggtcagg ttgttagtat atttttttac
     8281 aagccgtaat tgatccggag tgagtgaggt tccgccgctg gctaccacat tttcaattcc
     8341 tgcctggtgc aatgatataa cgtctgtata cccttctacc agcaaacatt catcagcttt
     8401 gtctatagcc tggcgggcaa aataggagcc ataaaggatc ttacttttga tgtaaatctc
     8461 attttctggt gtattgatat acttgggggc cttgtcatta ctttttaata tcctggcacc
     8521 aaatcccaat actttgccac tgtgattatg tatcggaaaa ataacacggc ccctgtaatt
     8581 atcaaccagc tgctcattac gattggcaac aaggccggat ttaattaaaa gttcagtatt
     8641 atactgtttt gcgattgctt cttttgtaaa agcatcccgc tgctccgggg aataacctag
     8701 ttggaatttc tttataatat cttctctgaa acctctttct ttaaagtaag caaggccaat
     8761 atcctgacct tcttctgttt caaaaagcat ccgggtgaag aattgttgtg caaaaccatt
     8821 gatgatgtac aggctatcag cagccagcat ttgctgccgc tgttcatcgt taacaaaagt
     8881 ttcctcaatt tcaattccat aacggttggc cagccatttt aaggcatcca cataactgta
     8941 cttctcatgc tccataataa aactcaccgt attcccgctt ttcccgcaac caaagcattt
     9001 atagatctct ttactcggag aaacagtaaa tgatggagtt ttttcattat gaaaagggca
     9061 gagtcccagg taattggtac ctcttttttt cagtttcaca aaactgccga cgacatcgat
     9121 aatatcgatt cggccaagta tctgttgtat ggtattagga gagatcatgt gcacaatatc
     9181 cggactgcga atataagtta tcggctgttt aagaaggaga aaaatcaccc gtccgggtga
     9241 gcaatctgcc gcccagatca ttgattttta tataaatatt acagcatgaa caaaatacta
     9301 accttgccat tcatcctgat cacacttatt gccagtgcca ataatgtgca gcttacgaac
     9361 ataagtgtaa ccaataatgg aacgaataca ggtaaaataa tagaatttga tctcacttgg
     9421 gaaaacagct ggcgcaccgc cagtaccggt aactgggatg gtgtttgggt attcttcaag
     9481 ttcaaagaca atgacggaac ctggtatccg ttgcatttca ccggtactga tatcataatg
     9541 cctgctggtg ctgcttacga aatgggaaat agtggcgttg tgaccggagt gggaatgttt
     9601 atctacaggg ctgccaatgg gtttggcaca gctgtggcaa ctggcattaa agccggtata
     9661 gagagttttc cgggtacgtt tgaaattaga ggatttgcac tggaaatggt ctttgtaccg
     9721 ggaggtagtt tttggctggg tgatgaaaca tcagtcaaca gttataaaga aggaagtacg
     9781 gcaaattcac ctttccaggt cactgcaaat ggagcgggaa ccaatatggg ttccagtaca
     9841 ggtttgttgt atgatcctca atcggcctac tcaggaaata tacccggatt tccaacaggg
     9901 tattccggat tctggatgat gaaatatgaa ctatcacagg gcggatatcg tgattatttc
     9961 aatacactta cttatacaca gcaattgaac aggcttcgtg atatattttc aaatccagca
    10021 tcgccaacag gaacaagtat caataatagt gctgcctgtt gcaggcaata tatggaaatt
    10081 gcaatcccgg gcaacagtac cacgaataca ccggcagtta ttgggcttga tgcagacgga
    10141 gataatatct ttaatgaggc tacggatgga gaatggatta ctgcaactta tttcacctgg
    10201 ccggatgtgg catcctacct ggattgggca ggattgagac ctatgacaga gttggaattt
    10261 gaaaaagcct gcagaggtcc tttaacaccg gttgctggcg aatttgcatg gggaaccaat
    10321 acaattgctt cttatccgta cacaattgca aatgctggta ctgccactga aaatattagt
    10381 aatatgagtg cgatactagg caactgcaat tcaggtactt ctcttgtatc ccttttaaga
    10441 ggaggcattt ttgctaccgg tgtttctacc agagtaagtg ctggtgcagg ttattacgga
    10501 acgatggaac tatcgggaaa cataagtgaa attgcaatca ctaccggcaa tgaggcggga
    10561 cgaagtttta atggcaaact tggtgatgga ttgcttacca ccgctggtaa tgccaatgaa
    10621 aataactggc ccggtgttaa tggtaatacc ggcactaatg ttgccccggg taactatgac
    10681 ggtggccttg gtgtaaggtc tgatggtggc attcgctggc ggggtggtgg ttttacgcaa
    10741 attaataatg atagttataa agtttctgac cgtattttat tttccggaaa cggtacagat
    10801 atttttacaa atcaaaccaa caaatcgttt gtaggaattc gtggagtaag agatgccaat
    10861 taaccagcta tgcagaaaat tcttttcaaa atatggataa ctctcattac cacaatccct
    10921 ctcagtatgc atgctcaatt tccctattcg ggaggcaatg ggaatggggc atcaggagtc
    10981 agttctgtaa atatcagttt gagtataact gacagcttgt acaatggggg aaatggcgat
    11041 ggtgttacta aaaatgtgtt gcttaatgct gtgttatcac aggctgatag tatgtataaa
    11101 ggcgggcagg gaaatggttt tcatagtatt gtattgacca ataccactct taatttattg
    11161 gatagcatat ataatggtgg aactggcaga ggtgataacc aaaaggttgc accagcgatc
    11221 aacctgagta tctgtacaga cgtactagta tggaatggca acgcaaatat caactgggac
    11281 aacccgggca actgggactg tggaacggtt cctggtgtta atagtattgt tattattccc
    11341 ggaggcaggc cccggtatcc tacaattttt actaatacag taataaagaa gctggagatg
    11401 cagcccggtg catctgttac agtattcacc aacaggctat tgttgattaa tggccaataa
    11461 ctttaaaggt aaagtcagca ttccctcttt ttaattggta tttatgggat ttcattcaaa
    11521 caaaatcacc cgggtgggtg acagtttgta tatcttattt taacagcttt acaataacca
    11581 tttactataa actatttttt atgaaaaagt tatttctcgc agtattattt gcaagtattt
    11641 ttgcctgtaa tttcttcagc tcagccatct tgtaagagta ttttttcagg cgcagagcgg
    11701 atttttaata acctgtcaca ggattttata aaattccgga tggatgcagc aaaggttttt
    11761 gtggatgtta taattcccgg taaacaaggc tcaaataaag ctatgaatga cctgattgac
    11821 gatgctacca acatgcagat tgatctgtat aaatcatacg gagtgattgc cggtcaaagc
    11881 aacgctacaa tcggcgcagt agacctgatt gtacctctga agaaatggac cggtgagctg
    11941 tatacagaaa ggacattcca gatcatgcaa tcgccttatg acaaaatcgt tatcacaata
    12001 aaaaaaacag atgggaaaag gggagtggag tttacggcct gtgccaaata cagtaacggc
    12061 tcgccttatg acactaaatc aggtaaaatt gaaaatggaa atgaaacggc aggccaggta
    12121 cgaaccataa ctttcccacg gaatatgctg gataaaaata tttaattaca cctggtggct
    12181 catggagctt tacctactga taaatgtgag tatacgctga cagttgaggg tttttttgat
    12241 gagaacgaaa tgaaagagat tgctaaaaca aatcctgctg aaaaggataa taaaaaaagc
    12301 cagttaaaag aaagtaatac ttcaccggcc aaagtttcaa gtggtaatca accagcaaaa
    12361 atgaatagca caaagcagcc tggggttacg aatgaaattg tatctccaag agaccctgct
    12421 tccgggctcc cagccggtaa aaaagtacag acggcccctg attcattaag tcaaaagaaa
    12481 aagaaaaaca attgataggt cattgtaaat tgaaatttac cgtcaaatgt ggataaaggt
    12541 gaattcgccc tactaatgta atatcgctta tttaaataca attctttatt cgtaaatgtc
    12601 cgtatgtgct gaagtccgga aaaaaaattg attactgagt aaaccaattt aaaaactagc
    12661 tgtctgatac caattaaatt tattttatga aaacatttaa gtacgtctac tcagcctttt
    12721 tctttacatg tatcatgttt gtaaatgtta cagcacaaaa ccagccaggt gttgattcta
    12781 ccggccttcc cggtgatgac ttcagcttac agggagcact gcagatgttt caggatgcaa
    12841 cgaccattga agattttgaa aaagcaatca atacagaggg gtatcatatt aacaacctcg
    12901 acctgaatgc tgatggagaa attgattata taagggtagt agataaatca gaaaaagatg
    12961 ttcatgcttt tgtactgcag gttccggttt cagaaaccga aaaccaggat attgcagtga
    13021 tagaattaga aaagacaggt gaagctaatg ccatcattca aataatagga gatgaggata
    13081 tttatggaga acaggtgatc gtagaaccgg atgggggcgg agaagaagcc ttttataatt
    13141 ttaatgacag caaactgagt ggccccaatg caggttatga ttttacaact cccgggattg
    13201 ttgtgaatgt ttggttatgg ccatcagtcc ggtttgtata tggaccagca tacaggccct
    13261 ggatttcacc ctggagatgg agatattacc ccggttggtg gaagccatgg aaaccattgc
    13321 gctggcatgc ctggcatccg ttcagggcac attatcacag gccattctct gtggtgcgta
    13381 cacatcgtgt aatacatgct caccgggttt atacaccctt ccgtacaact tcagttacgg
    13441 tccgtacccg gaattcagca gctgttggaa atttcagggt aacaagaaca aagaccagca
    13501 ttacagggcc gagaagaaaa acgacaacag tgaaaaagac cacagttaca ggacccaggg
    13561 gtggaaaagc tacaaaggtc aaagcgagga gacactaaaa ttaaaaaaca aaacattctt
    13621 tcataaggac accagctggt gtcctttttt attgataaaa tcacccgtca gggtgataaa
    13681 tggcttctga aggaaagata tttttatagt taactattac aactatgaaa agagtcattc
    13741 tcttcccttg cattaccggc attagtataa tttcattt
//
LOCUS       DAORTI010000092        13514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4471_length_13514_cov_6.707779, whole genome shotgun sequence.
ACCESSION   DAORTI010000092 DAORTI010000000
VERSION     DAORTI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4471_length_13514_cov_6.707779"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLO70_11560"
     CDS             complement(<1..414)
                     /locus_tag="PLO70_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQZ75148.1"
                     /translation="MSLSQSPYILYSDGKGNIFEDTSLYVTGRSGWDAMPVPEDEWIE
                     LPEGGSLYELPGRRGIGIDVETGEMRLCDKGWAVAAFIPPAHTGFYLAAYETAPDAPI
                     LPLFCYTAAGWLNEKFYVPAIRIEPDIRQECAGYDA"
     gene            complement(411..959)
                     /locus_tag="PLO70_11565"
     CDS             complement(411..959)
                     /locus_tag="PLO70_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75149.1"
                     /translation="MPRFQFLLKNEKLKQYNQIALYIILINLLAFAFIAIATTEKDIR
                     ISAIITVVILSSALAIDYFLGKIKNNEDTPYKLLAEYIIALAWLKAGFWWLALLFFIL
                     GMLYLGAKRKLLVSIFTDKILYPSLPQKNISWPELNAIILKDGLLTIDFKNNKLIQQT
                     IDENSTVNEQDFNEFCRQQLKK"
     gene            complement(961..2004)
                     /locus_tag="PLO70_11570"
     CDS             complement(961..2004)
                     /locus_tag="PLO70_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HQZ75150.1"
                     /translation="MVDLSSYDPNSVSNPNNNIFGFPTSEDNAKLVIIPVPWEVTVSY
                     GAGTARAPDSILKASYQIDLFDPEMPEAWKQGFYMRYVDKKILMKSDYLRKEAELYID
                     YISKGEELSANQFMCKTLKEVNEGGVFLKDWVFEQTKALLEKDKLVAILGGDHSTPLG
                     YMKAIGEKHGEFGILQIDAHCDLRDTYEGFHHSHAGIMFNALKEIPQLIRLVQVGIRD
                     YSEVEWDYIRNSNYKVITYFDKDINQRQFEGETWKSLTEEIVSKLPQQVYISFDIDGL
                     DRKLCPHTGTPVPGGFELEEVFFIFKKVLESGRKIIGFDLSEVGVSETDWNANVGARV
                     LYKLCNLLVTSNS"
     gene            complement(2131..3198)
                     /locus_tag="PLO70_11575"
     CDS             complement(2131..3198)
                     /locus_tag="PLO70_11575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQZ75151.1"
                     /translation="MTLPKFSGTSQSFHSELKKRISDYFNQAGKAPTGNFQLYFKAIF
                     LTVAFIAVYIHLVFYTPATWLALTECVVLGGLTAAIGFNVMHDGLHGSFSKHKWINNL
                     AGLSLNFLGANNFMWKTKHNIIHHTYTNIEGIDDDIEAKPLLRLCETQKYYKIHRFQH
                     WYFWAAYSLLYIWWVFFTDYKKYFLKRVGSVPLKPMSVGDHISFWGFKLLHAFLFVAL
                     PINIVGFTPWLIGFLVYGMFTGVVLSIVFQLAHTVEDTTFPHANIATHKMEDEWAIHQ
                     LKTTANFATGNKLISWLVGGLNFQVEHHLFPRVSHVHYPAISKIIKKACEDFGVTYIE
                     YPKMRIALASHVSHLKHLSHK"
     gene            complement(3328..4065)
                     /gene="bshB1"
                     /locus_tag="PLO70_11580"
     CDS             complement(3328..4065)
                     /gene="bshB1"
                     /locus_tag="PLO70_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HQZ75152.1"
                     /translation="MKVDILAIGVHPDDVELGCSGTLINEIKRGKKVAIIDLTQGELG
                     TRGTSETRYQEAANASMIMGVHARENLKMRDGFFKNDEDHQLKLISAIRKYQPAIVIG
                     NVLHDRHPDHGRAGKLIADSCFLAGLLKIETRDENNIVQPRWRPSYVLHYIQDWYHEP
                     DLLIDISDVFDQRMKAIEAYGTQFFTAETSHEGPQTYISTPDFLDSVIARARMFGKRI
                     GVKYAEGFISEKKIGIRNLDALIQNET"
     gene            complement(4170..4877)
                     /locus_tag="PLO70_11585"
     CDS             complement(4170..4877)
                     /locus_tag="PLO70_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HQZ75153.1"
                     /translation="MPEMTKYLKSIVLCATLTIMPGMIVIAQVQPPSVPVTDTNPPLP
                     PMSERMKNWGPNDTIIVPAIWYQREIVNYKEMDMAWVSNLSPEKLAKFIEEWTRLRNA
                     VYVTYPYARIAGATINDINANLAKVSSKKERKGYIKSREKELKKQFSDPLSNLSVYQG
                     KILMKLINRQTGNNCYEIIKEYKGGLNARFYQTVAFFVGGNLKQDWSIAKNKTDRDIE
                     NIVKEIDGSWYNNPYRR"
     gene            complement(4961..7426)
                     /gene="ccsA"
                     /locus_tag="PLO70_11590"
     CDS             complement(4961..7426)
                     /gene="ccsA"
                     /locus_tag="PLO70_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQZ75154.1"
                     /translation="MDYIGEHLLPGQLGHFFIVLSLVASLVATITYFLAAQSKTEADA
                     ISWKKLARYAFILEVISVFAIFGILFYIIHNHLFEYHYAWKHSSRSLEFKYLLACFWE
                     GQEGSFLLWSACHSVLGVILIKRSRNWEAPVMTIVSFAQFALATMVAGLYFFGWKMGS
                     NPFILLRDSGVLDNATGLHINFDVNQPLRPDYMLSVKDGNDLNPLLQNYWMVIHPPVL
                     FMGFASTIVPFAFAMAGLWKKQYGEWTKPALPWALFSAAVLGVGIMMGGMWAYESLTF
                     GGYWAWDPVENASLVPWMILVSGIHTLLIYRHSGHSLRATYLFFILAFGFILYSTFLT
                     RSGILGESSVHAFTDLGMNFQLLTFLLIFVLPSLAFFFARYNKIPSVQKEENTSSREF
                     WMFIGALVFFLSAIVIIGKTSLPVFNKIFGTNIAPPEDAEFAYNSIQIYVAIIMAALT
                     AVSQYLKYKDTSGKYFWKKILIPTIITLILSALFIVFGDIDYNKKGPVFQGSIWLAVV
                     CSIYTIVANAGYIWLGLKGNLKLSGGSISHVGFGMVLLGILISSSNKEILSNNIGGIP
                     APLAEGEDPRENLTLVKDMTANMGRYSLTYEGDSAHPKKQQWYYKVRFKSNDGKEEFV
                     LLPNAFVNYKGNEGLMANPSAKHYWDHDVFTYITSIPNPENEKDTSSFKPFTRKTGDS
                     LFYSRGFIIIQDVKQKDSIPEELFGADGTLYEVPLKIYSKTGSTYSVTSRLANAKGEW
                     FAIPDTITSESLVLQLQKVNPDKTIEMGVKESSAVLKYVTLKAYRFPFIKLLWYGVWI
                     TAIGILISMVRRIQLNRKNSISS"
     gene            complement(7498..7911)
                     /locus_tag="PLO70_11595"
     CDS             complement(7498..7911)
                     /locus_tag="PLO70_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQZ75155.1"
                     /translation="MKKIHIVLLVLIAGSIGVLLTFLQTASTYDTIDTAMAKPGKFVH
                     LMAKLDKSQPIEYDAVNNPNYLSFTAVDTLGKSVKVVYHNAKPDNLELSEKLVLKGKY
                     NDGKFECKDIQTKCPSKYKEEQAKGGQHPGTKETK"
     gene            8080..9237
                     /locus_tag="PLO70_11600"
     CDS             8080..9237
                     /locus_tag="PLO70_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQZ75156.1"
                     /translation="MVFQLNEEQLMIQKAARDFAQTECLAGVIERDEKQLFPREQISK
                     LADLGFMGMMVKPEYGGAGMDTISYVLAMEEISKVDASVSVCMSVNNSLVCYGLQEYG
                     TEEQKQKYLVPLAQGKKDGELYIGAFMLSEPEAGSDATSQRTTAEDKGDHYLLNGTKN
                     WITNGGTASVYLVIAQTDPAKGSKGINCLIVEKNWPGVVVAAKENKLGIRGSDTHTVM
                     FNDVKVPKENRIGEDGFGFKFAMKTLAGGRIGIASQALGIASGAYELAKEYSKTRKAF
                     GTEIMNHQIIAFKLADMATKIEAARLLCLKAAWEKDNGIDYTLSSSMAKVYASETAMW
                     TTTEAVQIHGGYGFVKEYHVERLMRDAKITQIYEGTSEVQRIVISRSILKG"
     gene            complement(9240..9887)
                     /locus_tag="PLO70_11605"
     CDS             complement(9240..9887)
                     /locus_tag="PLO70_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75157.1"
                     /translation="MNARYMAVSLLPLLFSITLVNAQQKYKGKLVSKLGQEQSGEITI
                     NLDGSNNELIEITYSEQTKTKGKRKSQQTTSMSMKLNVALIKHITINDTTYYFRDIKY
                     DYNDKYYMNVCVRLLDGTVNCGLFQQGRSTGTSNLSVKLPNSELSKLAAIDFDYYKAT
                     LGWHIFAFSKCKTLRSKMEEKQPGYYWDDNATPEQRLAMWKSWIQEYNSCSLQGG"
     gene            9945..10628
                     /locus_tag="PLO70_11610"
     CDS             9945..10628
                     /locus_tag="PLO70_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQZ75158.1"
                     /translation="MGILTMAANREAYYTICRELAITKTALIAVSKTKPVEDILELYH
                     LGHRDFGENYVQELVDKEAVLPKDIRWHFIGHLQSNKVKYIAPFVHLVHGVDSLKLLQ
                     EINKQAKKNNRIIDCLLQVYIAKEETKFGLDEQELEELVTQLNHQPFANIRICGLMGM
                     ASFTENMETVRSEFHYLKTLFEKLRISDAGLTTLSMGMSSDYSIAIEEGSTMVRIGSL
                     LFGERRKTG"
     gene            10691..11167
                     /locus_tag="PLO70_11615"
     CDS             10691..11167
                     /locus_tag="PLO70_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75159.1"
                     /translation="MRFYLPAIFIFFCISVNAQQERDTTLSQCPVFITDTVSSNNFFI
                     EARPATLKVYRVKGKLTIKVEQRDQFFNLYFHEKRLHNGKYKIRKGTGGKNEVEAAYT
                     FKSGDQSSYAAVSSGTVETLYDKEKKVWQLTVNGLIINQVERSATHYRVRAVLFIK"
     gene            complement(11180..11824)
                     /locus_tag="PLO70_11620"
     CDS             complement(11180..11824)
                     /locus_tag="PLO70_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75160.1"
                     /translation="MKPSLITLIIVIFSLSACAQKQEKATPEIDVGENCEGCEAVFES
                     PVPFNELNWIDTLPGFDEPGQKLAISGSVFKLDGKTPAAGIVLYVYHTNQKGIYPNKG
                     DENGWGKRHGYLRGWIKTNNSGQYKFYTLIPASYPNSNNPKHIHITIKEPGKNAYWID
                     DFLFGEDPLLSRELRNKNNFRGGNGILSTSHQKGIMIARRDIILGENIPGYPKE"
     gene            11997..13022
                     /locus_tag="PLO70_11625"
     CDS             11997..13022
                     /locus_tag="PLO70_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ75161.1"
                     /translation="MHNAPFFLKYKLHYVVFWMLIFGIWYSLRADSYPSEEKALLVTF
                     IKVVDLMLMISVTNYLLIPKLLYKKKYVLFGLAFLVLIISSSIIKMNILGRVLDNPLL
                     YNWEKNFKARVYDNIIPHFFLVIAGAAIKLMFDYGKLQQRMAATAKEKAEAELNFLKS
                     QINPHFLFNSLNSVYFLIDKDNADARGALHMFSEMLRYQLYEASGDKITIEKEIRYLK
                     DYMDLQQLRKDEKYAVQFSCSPGVKGVSIEPLLLIPFVENAFKHISHYTDKSNFVKVD
                     MQYANNVLTFLVENSKEKQKPDDKQGGIGLTNVKRRLELLYPGRYVLKIDESDERFIA
                     QLTIHLI"
     gene            13019..>13514
                     /locus_tag="PLO70_11630"
     CDS             13019..>13514
                     /locus_tag="PLO70_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ75162.1"
                     /translation="MIINCIIIDDEPLARKGLKEYIADIDFLALAGAFDSPLKATEMI
                     SSGQVQLLFLDIQMPKITGLDFFKSLQNPPPVVFTTAYPQFALDGFEVNALDYLVKPV
                     SFERFLKASLKAREYYEVREKNLTEAASSEFFFIKADNKLVKLLIDEVLFVEAMQNYV
                     MINTP"
BASE COUNT         4037 a         2635 c         2756 g         4086 t
ORIGIN      
        1 tgcatcataa ccggcacatt cctgccttat atcaggttcg atccgtatag ccggcacata
       61 aaatttttca tttaaccagc cggcagctgt atagcaaaac agcggaagga taggagcatc
      121 tggggctgtt tcataggctg ccagataaaa cccggtatgc gccggtggaa taaaggcggc
      181 aacagcccag cctttatcac aaagccgcat ttcgccggtt tccacatcta taccaatacc
      241 ccgtctgccg ggtaattcat ataaggagcc accttcaggt aattctatcc attcatcttc
      301 aggtaccggc attgcgtccc aacctgagcg gccggttaca tataatgaag tgtcttcaaa
      361 aatatttccc ttaccgtcgg agtaaagaat gtatggggat tgcgataaac tcatttcttt
      421 aattgctgcc tgcaaaactc gttaaaatcc tgctcattta cagtactgtt ttcatcaatg
      481 gtttgctgaa tgagtttgtt atttttgaag tcaatggtta agagaccatc ttttaaaatt
      541 attgcattca attcaggcca cgatatgttc ttttgaggaa gagaggggta aaggatctta
      601 tcagtaaaaa tgctgaccag cagttttctt ttggcgccca ggtacaacat acccagaatg
      661 aaaaataata atgccagcca ccagaaacct gctttaagcc aggccagcgc tatgatatac
      721 tccgctaata gcttataagg agtatcttca ttatttttta tttttcccag gaagtaatca
      781 atggcaagtg cacttgaaag tattactact gtaataattg cactgatccg tatatctttt
      841 tcagtagttg ctatagctat aaaagcaaaa gccagcaggt tgataagaat aatatataaa
      901 gcaatctgat tatattgctt tagtttttca ttcttcagga gaaattggaa tctgggcatt
      961 tcagctgtta cttgtcacca ataaattaca caatttataa agaactcttg ctcctacatt
     1021 tgcattccag tcagtttcac tcacacctac ttcactaaga tcaaaaccaa taattttccg
     1081 gccgctttcc agtacttttt tgaaaatgaa gaatacttct tccagctcaa atccgccggg
     1141 aaccggtgtg ccggtatgcg ggcaaagttt ccggtccaat ccatcaatat caaaactaat
     1201 ataaacctgt tgcggtagtt tactcacaat ttcttctgtc aggctcttcc atgtttcgcc
     1261 ttcaaattgc ctttgattta tatccttatc gaagtaggtt attactttgt agttactgtt
     1321 cctgatataa tcccattcaa cttcgctata atcccttatc ccaacctgta ccagccttat
     1381 caactgtggt atctctttca gcgcattgaa cattataccg gcatgcgaat ggtgaaaacc
     1441 ctcatatgta tcgcggagat cgcaatgggc atctatctgt aatatgccaa attcgccatg
     1501 tttttcgcca attgctttca tgtaaccaag cggtgtgcta tggtctcctc ctaatatggc
     1561 tactaactta tctttctcta aaagggcttt tgtttgctca aagacccagt ctttcaaaaa
     1621 aacgccgcct tcgttgactt ctttcagtgt tttacacatg aattgattgg cacttaactc
     1681 ttcccctttt gaaatatagt caatgtagag ttcagcttct tttctcaggt agtcgctttt
     1741 catcaatatc ttcttgtcta cataccgcat atagaagccc tgtttccacg cttcaggcat
     1801 ttcggggtca aacaggtcaa tctggtaact tgcttttagg attgaatctg gagccctggc
     1861 agtacctgcg ccatagctaa ctgtcacttc ccagggtacg ggtatgatca ccaattttgc
     1921 attgtcttca gaggtaggga atccgaaaat attattattg ggattgctta cactgttggg
     1981 gtcgtaggaa gaaagatcaa ccatattact gttattgaaa aacgttgcaa gttacagggg
     2041 tttgggatag cagcaaaatc aatcacccca aagtggacag attaaaaaag gagttgtact
     2101 acaactcctt ttttaatctg attcaataag ttatttatga ctaaggtgtt tcaggtgaga
     2161 aacatgggag gctaatgcta ttctcatttt gggatactca atataagtta ccccaaaatc
     2221 ttcacatgct tttttgatga tcttactgat ggcaggataa tgaacatgcg ataccctggg
     2281 gaacaaatga tgttcaacct ggaaattcaa acctccaact aaccaggaaa ttaatttgtt
     2341 gccggtagca aaattagccg ttgttttcag ctggtgtatg gcccattcgt cctccatttt
     2401 atgagttgca atatttgcat ggggaaatgt agtatcctca accgtatgtg ccaattgaaa
     2461 gacaatactc aggactactc cggtaaacat gccatataca agaaagccga tcagccaggg
     2521 ggtaaagccg actatattga taggcagggc aacaaaaaga aatgcgtgca ataatttaaa
     2581 gccccagaaa gaaatatggt cacctacaga cataggtttc aagggcacag acccaactct
     2641 ttttagaaaa tattttttat agtctgtaaa aaatacccac cagatgtaca acagcgaata
     2701 agctgcccag aaataccagt gctggaaacg gtggatcttg tagtatttct gtgtttcaca
     2761 aagacgtaat aatggctttg cttctatatc atcatcaatt ccttcaatat ttgtataagt
     2821 atgatgtatg atattgtgct tggtcttcca catgaaatta ttagcaccta aaaaattgag
     2881 agacaggccg gctaaattat tgatccattt atgcttacta aaactgccat gcaggccatc
     2941 atgcatcaca ttaaaaccga tggcagctgt taacccgcca agtaccacac attctgtcaa
     3001 agcaagccat gtagccggtg tgtaaaaaac aagatgaata taaactgcga taaatgcaac
     3061 tgtcaggaaa atagctttga aataaagttg aaaatttccg gtaggggctt tacctgcctg
     3121 attaaaataa tcactgattc ttttctttaa ttcactatga aaagactgag atgtgccaga
     3181 gaacttaggt aatgtcatta taatacaaat ttcaggttgt aaaggtaacc caaaacgacc
     3241 agatttctct aaaaattagt caaccgtttg ttatcggtca acaactgttt gtttaacatt
     3301 tgattcgcat ttacaggcaa acaaaagtta tgtttcgttt tgaatcaatg cgtccagatt
     3361 ccggatacca attttctttt cactgataaa tccttctgca tattttactc caattctttt
     3421 accaaacatt ctggctcttg caataacact gtcaagaaag tcaggagtgg agatataagt
     3481 ttgaggtcct tcgtggctgg tttctgctgt aaaaaattgc gttccgtatg cctcaattgc
     3541 tttcatccgc tgatcgaata catcactgat atcaatcagt aaatcaggtt cgtgatacca
     3601 gtcctggata taatgcagca cataggaagg cctccatctg ggttgaacaa tattattttc
     3661 atctctcgtt tcaattttca gtaaacccgc taagaaacaa ctgtccgcaa tcaattttcc
     3721 cgcccggcca tgatccgggt gcctgtcgtg cagcacatta ccaatcacta tagcaggctg
     3781 atatttacgg atagctgaga taagctttaa ctggtggtct tcgtcgtttt taaaaaaacc
     3841 atctctcatc ttcaggttct ccctggcatg aactcccatt atcatggaag cgttagcagc
     3901 ttcctggtaa cgggtctcac tggtgcccct tgttcccaat tcgccctgtg taaggtcgat
     3961 aatggctact tttttacctc tttttatttc attaatcagg gtgccggagc aaccaagttc
     4021 cacatcatcc ggatgaacac caatagcaag aatatctact ttcataaatg aagagaattt
     4081 aataaatgca aatgtatttg attacaatga gagtgtttgg gaagtggttg cagttctgtt
     4141 ttgaaagagc cggtggttga ggttaactat tacctgcggt aaggattatt ataccaggag
     4201 ccatctattt ctttcacgat attttctata tctctgtcgg ttttattttt ggcgatgctc
     4261 cagtcctgtt tgagatttcc tcctacaaaa aaggcaacag tctgataaaa tcgggcattt
     4321 aatcctcctt tgtattcttt gatgatttcg tagcaattgt ttcctgtttg ccggttgatc
     4381 aatttcatca ggatctttcc ctggtaaaca gaaaggttgg acagcggatc gctaaattgt
     4441 tttttaagtt ctttctctct tgatttgata tagccttttc tttctttttt tgatgaaacc
     4501 tttgcaaggt tggcattaat gtcattaatt gtagctccgg caatcctggc atagggatag
     4561 gtaacataga cagcattccg cagccttgtc cattcttcaa taaattttgc cagtttttcc
     4621 ggagataaat tggataccca tgccatatcc atttccttat agtttacaat ctcccgctgg
     4681 taccatatag ccggaacaat gattgtatca ttaggtcccc aatttttcat tctttcagac
     4741 atgggaggga gcgggggatt ggtatctgta acaggaactg aaggcggctg tacctgtgca
     4801 atgactatca tccccggcat aatggtaagg gtagcgcata acacaattga cttcaggtat
     4861 ttagtcattt ctggcatctg ctgtaaaaat agaaaatggt atcggtattt cagatagaga
     4921 cagtaaaact actaagtaag ctgtttcaaa aaagctaaaa tcaggaagaa atggaattct
     4981 tccggtttag ttgaatacgt cttaccatgc taatgaggat gccaattgct gtaatccaga
     5041 cgccatacca caaaagctta ataaagggga atcgataggc ttttagtgtt acatatttca
     5101 aaactgcact ggattctttt acgcccatct caatagtctt atccgggttt actttttgta
     5161 actgaagaac caggctctcg gaagtaatcg tatctggtat tgcaaaccat tcgccttttg
     5221 cgttcgccag ccgggaagta acagaataag tggatcctgt tttcgaataa attttcaaag
     5281 gcacttcgta taatgttccg tcagcgccaa agagctcttc tggtatactg tctttttgtt
     5341 ttacatcctg tatgatgata aagcccctgc tgtagaaaag cgagtcgccg gttttacggg
     5401 taaaaggttt aaaggaagac gtatcttttt cattttcagg attggggatt gaagtgatat
     5461 aggtaaatac atcatgatcc cagtaatgtt tggctgaagg attggccatc aggccttcat
     5521 tgcctttgta gttgacaaat gcatttggca ataatacaaa ttcttctttc ccgtcattgc
     5581 tcttaaatct tactttgtag taccattgtt gttttttggg atgtgcagaa tccccttcat
     5641 aggttaagga atacctgccc atattggctg tcatatcttt tacaagggtc aggttttccc
     5701 ttggatcttc accttctgcc aaaggagcgg ggataccgcc aatattattg gaaagtattt
     5761 ctttgttgga ggatgaaata aggatgccca atagcaccat accaaatcca acatgtgata
     5821 tagaacctcc cgatagctta aggttgcctt tcaaacccag ccatatataa ccggcattgg
     5881 ccacaatagt ataaatgctg caaactactg ccagccatat agaaccctgg aaaacaggtc
     5941 cttttttatt gtaatcgata tcaccgaaaa ctataaaaag ggcggataga ataagggtga
     6001 taatagttgg gattaaaatt tttttccaga aatatttacc ggaagtgtcc ttgtatttca
     6061 ggtattgact cactgctgta agggctgcca taattattgc cacatagatc tgaatgctgt
     6121 tgtaggcgaa ttcggcatct tccgggggag cgatattagt gccaaaaatt ttattgaata
     6181 ccggtaatga tgttttgccg ataataacaa tggcagaaag gaaaaagacc aatgcaccaa
     6241 tgaacatcca gaattccctc gacgaagtat tctcttcttt ctgaacagac gggattttat
     6301 tgtacctggc gaaaaagaaa gccagtgatg gcaaaacaaa aattaacagg aaagtaagca
     6361 gttgaaaatt catgcccagg tcagtaaaag catgtacgga agactctcct aaaataccac
     6421 tgcgggtaag gaaggtagaa tacaggataa aaccaaaagc aagtataaag aataaatatg
     6481 tggctctcag cgaatgaccg ctatgcctgt aaataagtaa ggtgtgaatt ccggatacca
     6541 agatcatcca gggtaccagt gaggcattct caacagggtc ccatgcccag taacctccaa
     6601 atgtgagtga ttcataagcc cacataccac ccatcatgat acctacaccc aaaactgcag
     6661 ctgagaataa ggcccaggga agagcaggtt tggtccactc cccgtattgt tttttccata
     6721 atcccgccat agcaaaagca aacgggacaa tagtagaagc aaatcccatg aacaataccg
     6781 gcggatgaat gaccatccag taattctgta ataaagggtt cagatcatta ccgtctttaa
     6841 cagacagcat ataatccggc cggaggggct ggttcacatc aaagttgata tgcaacccgg
     6901 tggcattatc caacacccct gaatccctta aaaggatgaa tgggttagag cccattttcc
     6961 aaccaaaaaa gtaaagacct gccaccatag tagccaatgc aaattgagca aagctgacta
     7021 ttgtcattac cggggcttcc cagttcctgc tgcgtttgat caggataact cctaaaacac
     7081 tatgacaggc actccagagc aggaaacttc cttcctggcc ttcccagaag caggcgagca
     7141 aatatttgaa ttccaaagaa cggctgctat gtttccaagc ataatgatat tcgaaaagat
     7201 gattatgaat gatataaaat aaaataccga agatggcaaa gacagaaatt acttcaagga
     7261 taaaggcata acgggccagt tttttccagg atattgcatc ggcttccgtt tttgactgtg
     7321 cagccaaaaa ataagtaatg gttgctacaa gggaggctac taaagaaagt acaataaaaa
     7381 aatgtcctaa ttgtccgggt aaaagatgct cgccaatata gtccatctca gaagtttaga
     7441 ttatgttact ttaaaaacgg gaagaggtta tttattgttc aatagacggg agagatatta
     7501 tttggtctct ttggttcccg ggtgctggcc gccttttgcc tgttcttctt tgtatttgga
     7561 aggacatttt gtttgaatgt ctttacactc gaatttccca tcgttatatt tcccttttaa
     7621 taccagcttt tcactcagct caagattatc aggtttggcg ttatggtaaa ctacttttac
     7681 agattttccc agggtgtcaa cggctgtaaa gcttaaatag ttgggattgt taaccgcatc
     7741 gtattcgata ggttgtgatt tatccagttt ggccatcagg tgaacaaatt tacctggttt
     7801 ggccattgct gtgtcaatgg tatcataagt actggcagtc tgcaggaaag taagcaaaac
     7861 gcctattgaa ccggcaatca gtactaaaag gactatgtgt atttttttca tggttattgt
     7921 ttgcagtgtg caaagttagg tcaaaaaaac caactgagat taaagggttt aacggcattt
     7981 acccggtgtt aatttcatta tatcttcctt tatttatcag gagtttttgc ccttatattt
     8041 gcattcctaa cactcgttcg aataataaat aatcgctata tggtatttca actcaatgaa
     8101 gagcaactta tgatccaaaa ggcagcccgt gattttgcac aaactgaatg ccttgccgga
     8161 gtaattgaaa gggatgaaaa gcaattattt ccccgtgagc aaatttcgaa actggctgat
     8221 cttggtttta tgggaatgat ggtaaaacca gaatatggcg gagccggaat ggatactatc
     8281 agctatgtgc tggctatgga agaaatcagt aaagttgatg caagtgttag tgtttgtatg
     8341 agtgtgaaca atagtcttgt gtgttatggt ctgcaggagt atggtacgga agaacaaaaa
     8401 caaaaatacc tggtgccgct ggcacagggg aaaaaagacg gtgagttata tattggcgct
     8461 ttcatgctca gtgaaccaga ggccggaagc gatgctactt cacaaagaac aactgctgaa
     8521 gataaagggg accattattt attaaacgga accaaaaact ggatcactaa tggtggaacc
     8581 gcatctgtct acctggtaat agcacagact gatcccgcaa aaggttcaaa agggatcaat
     8641 tgtcttattg tagaaaagaa ttggccgggt gtggttgtag ctgcgaagga aaataaattg
     8701 ggtatccgcg gaagcgatac acataccgtt atgttcaacg atgtgaaagt acccaaggag
     8761 aacaggattg gtgaagatgg ttttggattc aaattcgcca tgaaaacatt agctggtgga
     8821 agaattggta tcgcttcaca ggcactgggt attgccagcg gtgcttatga attggcaaaa
     8881 gaatattcca aaacaaggaa agcctttggc acagagatca tgaatcacca gatcattgcc
     8941 tttaaactgg ctgatatggc tacaaagatc gaagcagcaa gattactttg cctgaaagcg
     9001 gcgtgggaaa aagataatgg cattgattat acacttagtt cttccatggc caaagtgtat
     9061 gcatccgaaa cagcgatgtg gactaccact gaagcagtgc agatacatgg aggatatgga
     9121 tttgtgaaag aatatcatgt agaaagactg atgcgggatg ccaagatcac acagatatat
     9181 gaaggtacca gtgaagtgca gcggatcgtc atcagccgtt caatcctgaa agggtaaaat
     9241 cagcccccct gcagactaca ggaattatat tcctgtatcc agcttttcca catggcaaga
     9301 cgttgctcag gcgtagcatt atcgtcccaa taatagccgg gttgtttttc ttccatctta
     9361 ctacgtaaag ttttgcattt gctaaaagca aaaatatgcc agcccagtgt ggctttgtag
     9421 taatcaaaat caatagctgc cagtttagac aattcactgt tgggaagttt tacagacagg
     9481 ttggatgttc ccgtggaacg gccttgctga aacaaaccac aattgaccgt accatctaat
     9541 aaccgcacac aaacattcat ataatatttg tcgttgtaat catatttgat atcccgaaag
     9601 taataggtag tatcattaat ggttatatgt tttataagcg ccacatttag tttcatgctc
     9661 atagaagtag tttgctgcga ctttctttta ccttttgttt ttgtctgctc agaatatgtt
     9721 atttcaatca attcattgtt gctgccatcc aggttgatag tgatctctcc ggattgttct
     9781 tgtccgagtt tactcaccag tttaccttta tacttttgct gggcattaac cagggtgatc
     9841 gagaaaagga gagggagcag cgagaccgcc atatatcttg cattcataac tatatttgct
     9901 ttttgtaaaa ctatatttca attcaaattc aaacataccc gcttgtgggt attctgacaa
     9961 tggcagctaa cagggaagca tattatacta tttgcaggga gcttgcgata actaaaactg
    10021 cattaattgc tgtttccaaa acaaaaccag tagaagatat tcttgaactt taccatctcg
    10081 gtcatcgtga ttttggcgaa aattatgtgc aggagctggt tgataaagaa gcggtgttgc
    10141 cgaaagatat ccgctggcat tttatcgggc acttacagag taacaaagta aaatatattg
    10201 ccccctttgt tcacctggtc catggtgtgg atagcctgaa actgttacag gaaataaata
    10261 agcaggcaaa aaaaaataac aggatcatag attgcctgtt gcaggtctat attgctaaag
    10321 aggaaaccaa gtttggtctg gatgaacagg aactggaaga gttggtgact caattaaatc
    10381 atcagccatt tgccaatatt cggatatgcg ggttaatggg catggcttcc tttacagaaa
    10441 atatggaaac agtgaggtct gaattccatt atttgaaaac tctttttgaa aaactcagga
    10501 tcagcgatgc aggacttacg accctttcca tgggcatgag cagcgattat tcaatagcga
    10561 tagaagaggg tagtacgatg gtgagaattg gcagtctgtt atttggagaa agaagaaaaa
    10621 ccggctaagg gcagtcactt taatgaaata ttcatattta ttcgtatttt ttacaatcaa
    10681 tatcagcata atgcgttttt acctcccggc tatatttata ttcttttgca tttcagtaaa
    10741 tgcgcaacag gaaagagata ccacgctgag tcagtgccct gtttttatta ccgatacagt
    10801 tagttcaaat aattttttta ttgaggcaag acctgccaca cttaaagtat accgtgttaa
    10861 aggtaaatta accattaagg tggagcaacg ggaccagttc tttaatcttt attttcacga
    10921 aaaaagactg cataacggga aatataagat ccgcaaaggc accgggggga agaatgaggt
    10981 agaagccgcc tatactttca aatcaggtga tcaatcctct tacgctgctg tatccagtgg
    11041 aacggtcgag accttatatg ataaagagaa aaaagtttgg caacttactg tcaatggatt
    11101 gatcataaac caggtggaaa gatcggccac gcattacagg gtaagggcag ttttatttat
    11161 taaatgattg taaccatact tactcttttg ggtaacccgg aatgttttcc cctaaaataa
    11221 tatcccgccg ggcaatcatt atcccctttt gatggctggt actaagtatt ccatttccac
    11281 ccctgaaatt gtttttattg cggagctccc tggatagtaa aggatcttct ccgaacagga
    11341 aatcatctat ccagtatgca ttttttcctg gttcttttat ggtgatatga atatgtttgg
    11401 ggttattgct attgggataa gaggccggga tcagcgtata aaatttgtat tgcccgctat
    11461 tattggtttt tatccaccca cggagatatc catgtctttt gccccagcca ttttcatcgc
    11521 ctttgttcgg atatatacct ttctgattgg tatggtacac atacaataca atgccagcag
    11581 caggagtttt gccatcaagt ttaaaaaccg aaccactaat tgccagtttt tgtcctggct
    11641 catcgaaacc aggcagggtg tcgatccagt ttaactcgtt aaaaggtacc gggctttcaa
    11701 ataccgcttc acatccttca caattctctc ccacatcaat ttcaggagtt gctttttcct
    11761 gtttttgtgc acaggcactc aatgaaaata ttactatgat caaagttatt aaagacggtt
    11821 tcataagttt ttctattatc tggctagtga atttcgcagt gattttattg caggttcaat
    11881 taattacata aatggacagg tttgcctgat gaaatgcaca atgtagttag tcaagtaaat
    11941 acttccgtta atgtaatacc gtgtaagcac aggaaattgt acttttattt tgaactatgc
    12001 acaatgcccc tttctttttg aagtacaagc tgcactatgt cgttttctgg atgctgatat
    12061 tcgggatatg gtattccctg agagcagaca gttatcccag tgaagaaaaa gctctccttg
    12121 tgaccttcat aaaagtagta gacctgatgc tcatgatttc ggttaccaat tacctgctga
    12181 taccaaaact gctttataaa aagaagtatg ttttatttgg gcttgctttc ctggtactta
    12241 ttatttccag cagtattatt aagatgaata ttcttggcag ggtattggat aatccacttt
    12301 tatataactg ggaaaagaat tttaaagcaa gggtttatga taatattata cctcattttt
    12361 ttctcgtcat cgcaggtgca gctataaaat tgatgttcga ctacgggaag ttgcaacaac
    12421 ggatggctgc aacggcaaaa gagaaagcag aagcggaact taatttcctt aaatcgcaga
    12481 tcaatcccca ttttttattt aattccctca actctgtcta tttcctgatt gataaggata
    12541 atgctgatgc caggggagca ctgcatatgt tttctgaaat gctccgttac cagttatatg
    12601 aagcaagcgg cgataaaatt actattgaaa aagagatccg gtatctcaaa gattatatgg
    12661 acctgcaaca gttacgcaag gatgaaaaat atgcagtgca gtttagttgt tctcccggtg
    12721 taaaaggggt ttctattgag ccattactat tgataccatt tgtggaaaac gcttttaaac
    12781 atatttcaca ttatactgac aaaagtaatt ttgtgaaagt ggatatgcaa tatgcaaata
    12841 atgtcttaac ttttttagtt gaaaattcaa aggaaaaaca gaagccggat gataagcagg
    12901 gggggattgg gcttactaat gtaaaacgaa ggcttgagtt attatatccc ggcagatatg
    12961 tattaaaaat tgatgagtct gatgaacgtt tcattgcaca gctaactatt catttaatat
    13021 gataatcaat tgtattatta ttgatgatga accattggca agaaaaggcc tgaaagaata
    13081 tattgctgat attgattttc ttgctcttgc aggggcattt gatagccctt taaaggcaac
    13141 tgaaatgatc agcagcgggc aggtgcaatt acttttcctg gatatacaaa tgcccaaaat
    13201 aaccggactt gattttttta aatcgcttca gaacccgccg ccggtggtgt ttaccacggc
    13261 atatccgcaa tttgccctgg atggttttga agtgaatgct cttgactatc ttgtcaagcc
    13321 ggtttcgttt gagcgttttt tgaaagcatc attaaaggcg agggaatatt acgaggtaag
    13381 ggagaaaaat ttaacagagg cagcctcttc tgaattcttt tttatcaagg ccgataacaa
    13441 gttggttaag ctattaattg atgaggtatt gttcgtggaa gctatgcaga actatgttat
    13501 gatcaataca cccg
//
LOCUS       DAORTI010000093        13405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4536_length_13405_cov_7.499326, whole genome shotgun sequence.
ACCESSION   DAORTI010000093 DAORTI010000000
VERSION     DAORTI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_13405_cov_7.499326"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLO70_11635"
     CDS             <1..929
                     /locus_tag="PLO70_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQZ75163.1"
                     /translation="QGRGNPAFFFNSKTKYMNIQKAERKKAKIKLGLQGPSGSGKTFS
                     ALLLAYGLTEDWSKIVVIDTENHSASLYAHLGEYNVLHLQAPFSPEKYIDAIRHCITA
                     GMEVIIIDSISHEWEGNGGILETHSQMTGNSFTAWAKLTPRHNAFVQEILQSPVHILA
                     TIRAKQDYVLVDKNGKMVPEKVGLKGITREGMDYEFTIVFDLDIKHNATANKDRTSLF
                     IDKPEIRIDTSTGKIILEWCNSGSDITVDDISKRIGDCKSVQELFALYKMYPQFKASL
                     QAEYEAKKRRILINQETQTQLANQQFNKNGTE"
     gene            916..1917
                     /locus_tag="PLO70_11640"
     CDS             916..1917
                     /locus_tag="PLO70_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3871 family protein"
                     /protein_id="HQZ75164.1"
                     /translation="MEQNNIMDITFSGNSIQPPMSTDIPAEPTQQAFIVANTIESSVE
                     EIKGKHIIPVFTKDNETLISHTDFIDITADVIESVFDGERITKPQVRLSHPVKGRVPE
                     AKDKPANNLLEWEKTLYYERMMFIQEIPSIQETIDGNQLSLTVGGVKAYNQDNLYSRS
                     LCDQHFKVFIGFQNKVCTNLCVWTDGYMGEIKVKTIGQLKAAIRTLVEGYNKNFHLFH
                     LKKLTEYSITEQQFAHLVGRCRMYQHLPGEKKSEILPVLLGDQQIGTVVKDFYKDDSF
                     CRDEYGNINLWRLYNLFTSANKSTYIDSFLDRSVNAFQFVEQIRMAVDNKSSCWYLN"
     gene            2254..2703
                     /locus_tag="PLO70_11645"
     CDS             2254..2703
                     /locus_tag="PLO70_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HQZ75165.1"
                     /translation="MNELLVSEIKISYEPRVKASDSPKVTCSQDAYEQIKQFFDPSLM
                     NVKEEAVVLYLNCSNRIMGGFKVSSGGISCTIVDITLILGVAVKCLARGLVLAHTHPS
                     GELQPSKADLNLTARLKEAARLLDIRLIEHLILSTDGYLIFADEGLL"
     gene            3538..4689
                     /locus_tag="PLO70_11650"
     CDS             3538..4689
                     /locus_tag="PLO70_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75166.1"
                     /translation="MNLQITAWMFILVLVAGCSSSNKIDKRYTADDKTVFDLMDRLKK
                     NANDAEAAKLLPDAYLQAAETRKNINKDTYNNMNEGDRWIEIAKQLEVAEKMYTEISS
                     SPVLSKIIPDPWNPGEKIRDAKLKAAAEYYNQGLAFLNYNNRPYAQKAYDMFVKANNA
                     YPNYKEVRSLLEQAKELATIKVVVRPVNYYNFGWNYWGFDRDYLQYKMVQDLNSSSSY
                     QHVRFYTDRDADARRIRADRIVELNFNDLYISPVYRDEYTIPRSKKIQTGQTKSNPPQ
                     PIYETVYATVYVTRQVLQSRASLECRIYDWATGRNYLSDRFPDNYTWREERARYSGDS
                     RALEPSDWALINNNSGFNAPVRNQIAERLINNCYNQLLSRIRSGVTFGD"
     gene            4725..5648
                     /locus_tag="PLO70_11655"
     CDS             4725..5648
                     /locus_tag="PLO70_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQZ75167.1"
                     /translation="MKKITTLTLMLLPLFIMAQDGTVKALRSEAERTIKKEEDTTKRL
                     WRKGGLYGINISQGSLSNWAAGGDNFSLSVNSLLSLYAFYKKDKHSWDNTFDFNIGYV
                     KTTSLGSRKNDDRFDLLSKYGYALNPKLNLSGLVSLRSQLFKGYTYPDNVKSFSSAFM
                     APGYLLTSIGLDYKPTKNLSIFLSPVTARWVIVRDTALANKGLYGVTPGKKTNLEFGA
                     FASINYLKEFNKFITYKSRLDLFSNYRHNPQNIDLFMSNILNAKLGKVLSVSWSLDFI
                     YDDDVRLFGKNQRSAAMQVKSLIGIGFLLKF"
     gene            5862..6791
                     /locus_tag="PLO70_11660"
     CDS             5862..6791
                     /locus_tag="PLO70_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQZ75168.1"
                     /translation="MNRKVLSFLLFLLPLTAFSQDAIVKKLQMESLRQVKKDIPDTGW
                     KKWRTGGAYSLSIGQGSQSNWAAGGDDFSFTVNTSLRLFAFYKQNKNSWDNTFDVNFG
                     YINTTSLGSRKNDDRFDLLSKYGFALNKKFNLATLANFRSQFLKGYTYPDNVKTFASA
                     FLSPAYLVVSQGIDYKPNKELSIFLSPVTSRWVFVTDSVLSAKGEYGVSYGTNSYNQN
                     GAFATISYNKSFSKYVSYKSRLDIFSNYKKDPQNIDIFMTNTLSAKVAKIIAFSWNVD
                     MIYDNDVQLFGKTNSSPALQLKSIIGIGLQVKI"
     gene            6853..7563
                     /gene="trmB"
                     /locus_tag="PLO70_11665"
     CDS             6853..7563
                     /gene="trmB"
                     /locus_tag="PLO70_11665"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQZ75169.1"
                     /translation="MGQKKLIRFAELETFSNVLQFPKDMPGKWSVFFGNNNPLTLELA
                     CGKGEYAVGLGQLYPNRNFIGIDLKGNRIWVGAKRALQSKLTNVAFLRTQIDQIAEYF
                     APGEVAEIWITFPDPQLRMSKAKKRLTHPKFLRLYQQFLVPGGLIHLKTDSPDLYRFT
                     KLVIDLYGCPLHKDMDDIYETEKISDELKIKTHYESLDIAGSNRIHYLCFSIPPDLGN
                     KEKDTELKEKLKEYEGLD"
     gene            7547..7735
                     /locus_tag="PLO70_11670"
     CDS             7547..7735
                     /locus_tag="PLO70_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HQZ75170.1"
                     /translation="MKDLIEGTHYYINEEGYVVLTEQYHLEKGYCCGNGCKHCPYEYE
                     NVPEPKKSILQQEKENSK"
     gene            7751..8140
                     /locus_tag="PLO70_11675"
     CDS             7751..8140
                     /locus_tag="PLO70_11675"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQZ75171.1"
                     /translation="MTVRKKPSGKIKTIKPSGKREENFFALVFEVARQIPKGRVTSYG
                     AIAACLGTKSSARMVGWAMNGCHLVKPKVPAHRVVNRNGMLTGKHHFSPPGMMEKLLK
                     KEGIKVKNDQVIDFDKHFWNPAEELGL"
     gene            complement(8169..>8647)
                     /locus_tag="PLO70_11680"
     CDS             complement(8169..>8647)
                     /locus_tag="PLO70_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQZ75172.1"
                     /translation="IVFAAVVLLGRVKLEVDLGFDVHFFARANATINSIVAVLLVFAL
                     VAVKSKKYQLHKTLMMAAMLLSVLFLLSYICHHLFAGETKFGGEGISKTIYYIILFTH
                     IPLAGLILPFILFTAYRALISEWPQHKKLAKITWPIWFYVAVTGVVVYWMISPYYV"
     assembly_gap    8648..8747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8859..9665)
                     /locus_tag="PLO70_11685"
     CDS             complement(8859..9665)
                     /locus_tag="PLO70_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQZ75173.1"
                     /translation="MNKTALYAVMIALLVPLISYFIVKTKSESAIHMPRHYFPDSVVT
                     DTRKGKKVTDTVWHKAGDFNLTNQNGMPVSWDSLKGKIIIADFFFTRCPTICPGMTMN
                     MKRLEESIHNGKRVGDKTNKLVHFLSFSIDPERDSVERLKYWANRFQIDPEKWWLLTG
                     DKKAIYDFALNEVKLGLIDGDGVDTNFVHSDKFILIDSNRHVRGYYSGLDSVSLSKLS
                     NDLVLLTMEKDPNRKSFFAGKLQVMAVSFLLAIMGVFLLLFFLRKNKKNA"
     gene            complement(9754..10212)
                     /locus_tag="PLO70_11690"
     CDS             complement(9754..10212)
                     /locus_tag="PLO70_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQZ75174.1"
                     /translation="MQHPAFEEVTIHHHTIDDAAFKKKVRKTTILLSVITVIELAIGL
                     LIYNIHKGENPSELLVLMFKGMVCILTLAKAYYIVSVFMHLGDEIRNMIMTIVVPLLL
                     FIWFIAAFIWDGNSYKNLRNTYDKHFQETTMPAKHHKEEAKPVEKPTGKE"
     gene            complement(10272..11144)
                     /locus_tag="PLO70_11695"
     CDS             complement(10272..11144)
                     /locus_tag="PLO70_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQZ75175.1"
                     /translation="MATTATVHQTKWWSGGKSPFNLEYGKVMMWYFLMSDAFTFGAFL
                     ISYGSIRFSQNFWPDPNVVFNAFPGAGHANLPLAFVSVMTFILIISSVTMVLAVHAGH
                     KGDKQGVIKWLFWTIIGGLAFLGCQAWEWHHLITGEHAVLIDGQIDIIGQTTRANPWG
                     DLVSNEAAMAALQKTPHETLVNLAHQSNHAVSHEQLVAMTDQQLIKEIKLDELHIRSK
                     GPVAFGGYFYGITGFHGFHVFSGVIINIIMLMMTQKNVFQNRGHYLMIEKAGLYWHFV
                     DLVWVFVFLCFYLI"
     gene            complement(11180..11752)
                     /locus_tag="PLO70_11700"
     CDS             complement(11180..11752)
                     /locus_tag="PLO70_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQZ75176.1"
                     /translation="MDIVSNQHKQKMHPHKFTLWVAIGSLVMMFAGLTSAFIVKSNQV
                     NWKPVTLPKVFWISTIVILLSSLAVQLALRSFKNREMNKYRMLIGATVLLGSVFIVFQ
                     WMGFQNLWAQQVTFRGAGAGQFLYVIFSLHALHVIGGIVALMVMLIKAFFGKIKLYSS
                     VPVEVAATYWHFVDFLWIYLLVFFLVIGGN"
     gene            complement(11774..12649)
                     /gene="cyoE"
                     /locus_tag="PLO70_11705"
     CDS             complement(11774..12649)
                     /gene="cyoE"
                     /locus_tag="PLO70_11705"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQZ75177.1"
                     /translation="MKKIKDYLLLIKPSLSIMVVFSSVISYLLAPKVVEYNWIMILLL
                     FAGGMLVTGSANAINQVVEKDTDAQMKRTSTRPVAAGRMSVGEGWAFAIVTGALGVFI
                     LGYYFNWLSAGLAAFSLFLYAFIYTPLKKVNSIAVLMGAVPGALPCLIGWAAGDDQLS
                     TGGWILFAFQFFWQFPHFWAIAWVAHKDYSAVGFKLLPANEGPTRFTAIQTIAYSLLL
                     VPVTLLPYFTGMCSYEDTMGLISIGLVILANLFMIGRCVALYKNMDVSSARKVMFGSY
                     MYLPVVLLALLLSKA"
     gene            complement(12719..>13405)
                     /locus_tag="PLO70_11710"
     CDS             complement(12719..>13405)
                     /locus_tag="PLO70_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQZ75178.1"
                     /translation="KVFNWLTTLWRGNIRFTPATLFAIGFVSLFISGGLTGIFLGNST
                     IDIHLHDTMFVVAHFHIVMGVASFFGMFAGVYHWFPKMFGRYLNNTLGYMHFWVTMIG
                     AYLIFWPMHYQGLAGIPRRYLDKSGWVSFNQFGELDKMITIVSIIVFAVQLMFVFNFF
                     YSIFKGRKVTTPNPWGANTLEWTTKINPGHGNWEGEIPEVHRWPYDYSKDGRDFIPQT
                     EPIGANESKH"
BASE COUNT         4198 a         2870 c         2568 g         3669 t
ORIGIN      
        1 ttcaaggcag gggcaaccct gcctttttct ttaactcaaa aacaaagtat atgaatattc
       61 aaaaagcgga acgcaaaaag gcaaagatca aactcggact ccagggtcca tccggaagtg
      121 gtaaaacctt cagtgcatta ttacttgcat atggacttac agaagactgg agtaagatcg
      181 tagtcattga cacagaaaac cattctgcaa gcttgtatgc ccatctcggt gaatataatg
      241 tgctgcacct gcaggcaccc ttcagtcccg aaaagtacat tgatgcaatc aggcattgca
      301 ttactgcagg aatggaagta attattattg attcaataag ccatgaatgg gaaggaaacg
      361 gcgggattct tgaaacccac tcgcaaatga caggcaattc ctttaccgca tgggctaaac
      421 ttacaccccg tcataacgcc tttgtccagg aaatacttca gagtccggtt cacatattgg
      481 ccaccatcag ggcaaaacag gattatgttt tggttgataa gaacggtaag atggtcccgg
      541 aaaaagtagg attaaaaggg atcacccggg aagggatgga ttatgaattc accatcgttt
      601 tcgatctgga catcaagcat aacgctacgg ctaataagga caggacttcc ctgtttattg
      661 ataagcccga gatcaggatt gataccagta ccggcaagat tattctggaa tggtgtaaca
      721 gcggctctga tataacggtg gacgatatat ccaaacgtat tggagactgt aaatccgttc
      781 aggaactctt tgctctttac aaaatgtatc cccaattcaa ggcttcactg caagcagaat
      841 acgaagcaaa gaaaagaagg atcctgataa atcaggaaac tcaaacacaa ttagctaacc
      901 aacaattcaa caaaaatgga acagaataat atcatggata tcactttttc cggtaatagc
      961 attcaaccac ccatgagtac agatatccca gcagaaccca cacaacaggc atttatagta
     1021 gccaatacca tcgaaagttc ggttgaagag atcaaaggca aacacattat cccggttttt
     1081 acaaaggata atgaaacgct gatttcgcat accgatttta tagatataac agctgacgtt
     1141 atagaaagtg tttttgatgg ggaacggatt acaaagccgc aggttcgatt atctcatccg
     1201 gtaaaaggac gagtacctga agcaaaggac aagcctgcca ataacctgct ggaatgggaa
     1261 aagactctct attatgaaag gatgatgttc attcaggaga tcccatcgat tcaagaaaca
     1321 attgatggaa accagctatc gctaaccgta ggcggtgtaa aagcttataa ccaggacaac
     1381 ctgtacagcc ggtctctttg cgatcagcat ttcaaggttt tcattgggtt tcagaataag
     1441 gtttgcacca acttatgcgt ctggacagat ggttacatgg gagaaatcaa agtgaagact
     1501 attggccagt tgaaagctgc aattcggacc ctggtagaag gatacaacaa gaacttccat
     1561 ctatttcatt tgaaaaagct caccgaatac agtatcactg agcagcagtt tgctcacctg
     1621 gtgggcaggt gccggatgta ccagcattta ccgggagaga agaagtctga gatactacca
     1681 gttctattag gcgaccagca aattgggaca gtagtaaagg atttctacaa ggacgattcc
     1741 ttttgccggg atgaatacgg gaatatcaat ttgtggaggc tttacaacct gttcaccagc
     1801 gcaaacaaat cgacctacat cgatagcttc cttgaccggt ctgttaatgc ctttcagttt
     1861 gtagaacaga tacggatggc ggtcgataac aaatcaagct gctggtattt gaactaaatc
     1921 aagcaaatca tttttacgta tggcaaaagc taagctgcgg gtgtattacc atgatccgtg
     1981 gagtatcctc gtgataaatc ccagggggca agtaagaaag ctctatactc ccttcagagt
     2041 gctttgcatt gaaccagcaa ccaactggct taagaagggc gcctgggtct ttgtcgatga
     2101 agtaaaatca tccgacaagg atgagctaat atttattgtt ggtggacacc agtttcttca
     2161 tcggcacttt cggatccaaa tcaatttcta atcaaatttc atcacctact gtccgtctgt
     2221 tagtaaagca ggcggacatt ttaattattc aacatgaatg agttattagt ttctgaaatc
     2281 aaaataagtt atgagcccag ggttaaagcc tcggatagcc caaaggttac atgctcccag
     2341 gatgcttacg agcagataaa gcagtttttt gacccttcat taatgaatgt taaggaggag
     2401 gcggttgtgc tgtacttaaa ctgtagcaat cggattatgg gaggattcaa ggtttcttct
     2461 ggcggtattt catgtaccat agtggatatc actctgatcc tcggtgtcgc tgtaaagtgc
     2521 ctggcaaggg gcttggtttt agctcatacc catccttctg gagaattaca gccgtcaaaa
     2581 gctgacttaa atctgacagc aaggttaaag gaggctgcca ggcttttgga tatcagactt
     2641 attgaacatt tgattttgag tacggacggc tatttaatct ttgccgatga aggactcttg
     2701 taataattct gaatactaaa gattgcaatc cctctcgcag acccggtcgc acacccggca
     2761 aattcaccct tttatagaaa tcttctttta aaacatagaa tataggggaa gacactttat
     2821 atagcttaaa ccatcatttt atgacgatca taaatatatt tttaataacc tttcttctca
     2881 ctacttcttt gattgttggc tatatagcca gaagggaggc agggatgaga tgtaaatcag
     2941 atacagctga atttgttctc tgtataatga caggcgttat aattctggga attcttatgg
     3001 tgctattcta gaaatatgcc ccggttagcc actcctggtg ccctggaatc tgatatagtt
     3061 ttccaatctc gctatgagtt caggatagca cctaaaaaac gcatctgctt ccaaatgaag
     3121 taatgcccgc cctaataatt aggggattct tgaggccata aatgcgtctc aggcatataa
     3181 cgagtcacgt tatatatttg tagtagtaat tgaaaaacag gtaacctttt cggtaacctc
     3241 ccgctttttg aatggattgt ttgaaggctt tttggcagac atgccctctt gtctcggggg
     3301 tggggaaaac aggataaata tagcatagag ccgttttggt agcaataccg gaacgaccag
     3361 ggtcgaccac tgaactttgt gctacaacta actgccccgt ggaggttcga tccctccccc
     3421 tacagcactt ttttcatgcg attttagcct ttgcattttt tgcaaaggct tttttatttt
     3481 tgtactgcct cactataata tttaccatta atcctttttt tcttttaata acttcttatg
     3541 aatttacaaa tcacggcatg gatgtttatc cttgttttag tagcaggctg ctcctcctct
     3601 aataaaatag ataaaagata taccgcagat gacaaaaccg tttttgacct gatggacagg
     3661 ttaaagaaaa atgccaatga tgcagaagcc gcaaaattgt tacccgatgc ttacctgcag
     3721 gcagccgaaa cccgaaaaaa tatcaacaag gacacttaca acaatatgaa tgaaggtgac
     3781 cggtggattg aaatcgccaa acaactggag gtagctgaaa aaatgtatac tgaaataagc
     3841 agcagccccg tgctgagtaa aataattccc gatccatgga accccggcga aaaaatccgg
     3901 gatgccaaat tgaaagccgc tgcagaatat tacaaccagg ggctggcgtt tttaaactat
     3961 aacaaccgtc cttatgcaca aaaagcatac gacatgtttg taaaagccaa taatgcatat
     4021 cccaattata aagaggtaag atcattactg gagcaggcaa aagaactggc tactattaaa
     4081 gtggtggtcc ggcctgtcaa ttactacaat tttggttgga attattgggg ctttgaccgt
     4141 gactaccttc aatataaaat ggtgcaagac ctcaatagtt cttcctcgta tcagcatgta
     4201 cgtttttata cggacagaga tgctgatgca cgacgtatcc gggccgaccg gattgtagaa
     4261 ctaaacttca atgacttgta tattagtcct gtctaccggg atgaatatac tataccccgc
     4321 tcaaaaaaaa ttcaaacggg acaaaccaaa tcaaacccgc ctcagcccat ttatgaaacc
     4381 gtgtatgcca ctgtttatgt aacaagacag gtattgcaaa gcagggcttc tctcgaatgc
     4441 cgtatttatg actgggccac cggccgcaat tatttatcag accgtttccc cgacaactat
     4501 acctggagag aagaaagagc aagatatagt ggtgacagcc gtgctcttga accttccgat
     4561 tgggcactta tcaataataa ttccggattt aatgcaccgg tacgcaacca gattgccgaa
     4621 cgccttatta ataattgtta caaccagctt ttaagccgta tcagaagtgg tgttacgttt
     4681 ggagattaat ctttactaaa tacagtacaa atcaaaacaa ctttatgaaa aaaattacaa
     4741 ctcttacgct aatgctccta cctcttttta taatggctca ggatggaaca gttaaagcgc
     4801 tccgttccga agcagaacga acaattaaaa aagaggagga taccactaaa agattatgga
     4861 gaaaaggagg actctatggt atcaatatat cacaaggctc cctgagcaat tgggcggccg
     4921 gtggtgataa tttttctctt tctgttaatt cattactcag cctgtatgca ttttataaaa
     4981 aagataaaca tagctgggat aatacatttg attttaatat tggttatgta aaaacaacca
     5041 gcctgggcag ccgaaagaac gatgaccgtt ttgacctgct ttcaaaatat ggttacgcat
     5101 tgaaccccaa gcttaatctc tcgggtcttg tcagtcttcg ttcacaatta tttaaaggat
     5161 acacttatcc tgataatgta aaaagttttt cgtctgcatt catggcaccg ggttatttgt
     5221 taaccagtat tggtctcgat tataaaccca ctaaaaatct ttctattttc ctttcaccgg
     5281 taacagcccg ctgggtgatt gtaagagata ccgcccttgc caataaagga ttatatggag
     5341 taacccccgg caaaaagacg aatcttgaat ttggtgcatt tgcgtctata aactacctga
     5401 aagaattcaa caagtttatt acttacaaaa gcaggctgga tttgttttca aactacaggc
     5461 acaatccgca gaatattgac ttgtttatgt caaatatcct gaatgcaaaa ctgggtaaag
     5521 tattatcagt gtcctggagt ttggatttta tttatgacga cgatgtaaga ttatttggta
     5581 aaaatcagcg gtcagccgcc atgcaggtca agtccctgat cggcatcggg tttctgctga
     5641 aattctgata tatatatcat agtagcaaaa gccacattca ctatataatg aatgtggctt
     5701 ttttattata caaacactca ttagcatcag ctatgtttta acattctgta tgaaataatt
     5761 tatttctttc tgtgtttctt tgtacttata tctttgctgc accctgctga aaacaattac
     5821 actgtacaac ccctttttct aaagtaaacg aaccaatact gatgaacagg aaagttttat
     5881 catttctatt gtttttattg ccactaactg ctttttcgca ggacgctatt gtaaaaaaat
     5941 tacaaatgga atctctccgg caggttaaaa aagatatccc ggataccgga tggaaaaaat
     6001 ggagaaccgg tggagcctac agtcttagta tcgggcaagg aagccagagt aactgggctg
     6061 ccggcggtga cgacttttct tttactgtta atacctcgct ccgcctgttt gcattttata
     6121 agcaaaacaa aaatagctgg gataatactt ttgatgtaaa ctttggctat atcaatacaa
     6181 ccagcctggg cagccgcaag aatgatgacc ggtttgacct gctttcaaaa tatggatttg
     6241 cgctaaataa aaaattcaac ctggccaccc tggctaattt ccgttcacaa tttctgaaag
     6301 gctatactta tcctgataat gtaaaaacat ttgcatccgc ttttctttcc ccggcatacc
     6361 tggtagtgag ccagggtatt gactacaaac ccaataaaga gctttcaata tttctttctc
     6421 cagtcacttc ccgctgggta tttgtcacag atagtgtctt atctgccaaa ggtgaatacg
     6481 gcgttagtta tggcacaaac agttataacc agaacggggc atttgctacg attagttata
     6541 acaaatcgtt cagtaagtat gtttcgtaca aaagcaggct ggatattttt tccaactaca
     6601 aaaaggatcc gcagaacatc gatattttta tgaccaatac attatctgcc aaagtagcaa
     6661 agatcatcgc cttcagctgg aacgtggata tgatctatga taatgatgtg caactttttg
     6721 gtaaaacaaa ttcttcaccg gcactccaac tgaaatctat cattggtatc gggttacagg
     6781 taaagatctg acacttcatt caaatcaact gtcaacttcc gcccctgaat tttgggctat
     6841 atttgccgcc caatgggtca gaagaaatta atacgctttg ctgaactgga aaccttcagt
     6901 aatgtgctgc agtttccaaa agacatgcca ggaaaatggt cagtcttttt tggtaataat
     6961 aacccgctta ccctggaatt agcctgtgga aaaggtgaat acgctgttgg cctgggacaa
     7021 ctttacccca atcgaaactt cattggtata gacctaaaag ggaaccgcat atgggtaggt
     7081 gccaaaaggg ctttacaaag caagctgacc aatgttgcat tccttcgcac acagatagat
     7141 cagatagcag aatattttgc tcctggtgaa gtagcagaga tatggatcac ttttcccgac
     7201 ccgcaactgc ggatgtcaaa agcaaaaaaa aggctgactc acccaaaatt cttacggctt
     7261 taccaacaat ttctcgttcc cggtggttta atacatctga agacagacag ccctgatctt
     7321 tatcgtttta ccaaactggt gattgatctt tacggttgcc cgttgcacaa agacatggat
     7381 gatatatatg aaacggaaaa gataagtgat gaattaaaaa ttaaaacaca ttacgaatca
     7441 ctggatattg ccgggagtaa ccgaattcat tatttatgct tctcaatacc tcccgatctc
     7501 ggtaataaag aaaaagatac agagctgaaa gaaaaactga aggaatatga aggacttgat
     7561 tgaaggaacg cattattaca tcaatgaaga gggttatgtg gttttaacag aacagtacca
     7621 cctggagaaa ggatattgct gtggcaatgg atgcaagcat tgcccttatg aatatgaaaa
     7681 tgttcctgaa cccaaaaaat caatactgca gcaagaaaag gaaaacagta aatagattta
     7741 tcttcatgac atgactgtca gaaagaaacc atccggaaaa ataaaaacaa taaagccatc
     7801 cggtaaaagg gaggaaaatt tctttgcgct tgtttttgaa gtagcccggc aaataccaaa
     7861 aggaagagta accagttatg gcgccattgc agcctgcctc ggcaccaaat cgtctgccag
     7921 aatggtaggt tgggccatga acggatgtca cctggttaaa ccaaaagttc ctgcacatcg
     7981 tgtagtaaac agaaatggca tgcttaccgg taaacatcat ttctctccac cgggaatgat
     8041 ggaaaagtta ttaaagaaag aaggcatcaa agtgaaaaac gaccaggtga ttgacttcga
     8101 taagcatttt tggaatcctg cagaggagct ggggttgtaa gaaaaaggaa aactattaca
     8161 gaaagatctc aaacataata cgggctgatc atccaatata ccaccactcc ggttactgcc
     8221 acataaaacc agataggcca ggtgatcttt gccagttttt tatgctgtgg ccactcactg
     8281 atcaatgccc tgtaagcggt gaataaaata aaaggaagga tcaatccagc cagcgggata
     8341 tgggtgaata gaataatata atagatggtt ttactgatgc cttcaccgcc aaatttagtt
     8401 tcaccagcaa ataaatgatg acagatataa cttagtaaaa ataaaacaga caaaagcatc
     8461 gctgccatca tcagcgtctt atgcagttga tatttttttg actttacagc taccagtgca
     8521 aataccagta ataccgcaac tattgaatta atcgttgcat tggcccgggc aaaaaaatgc
     8581 acatcaaaac ccagatcaac ctccagttta accctgccca gtaatactac cgctgcaaat
     8641 acgataannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga tcaacctcca
     8761 gtttaaccct gcccagtaat actaccgctg caaatacgat aaaggaaaag agataaatta
     8821 atgttcttgc ttttttatca ttctttgtaa gaacaggctt aagcattttt tttatttttt
     8881 cgtaaaaaga aaagtaacaa aaacacaccc atgatagcca ataaaaacga tactgccatt
     8941 acctggagtt tacccgcaaa aaaactctta cggtttggat ctttctccat cgtcagtaac
     9001 accaggtcat tggacaattt tgataaagac acagaatcca gcccgctgta ataacccctt
     9061 acatgacggt tactgtcaat cagtataaac ttatcactgt gtacaaaatt ggtatccaca
     9121 ccgtccccgt caataagacc cagtttcact tcattcaacg caaaatcgta aatagctttc
     9181 ttatcaccgg taagcaacca ccatttttca ggatcaatct gaaaacggtt ggcccagtat
     9241 tttaaacgtt ctacagaatc cctttccggg tcgatgctga acgaaagaaa atgtaccagt
     9301 ttattggtct tatctcccac tctttttcca ttatgtatcg attcttccag tcgcttcatg
     9361 ttcatcgtca tacccggaca aatagtaggg caacgggtaa agaaaaaatc tgctatgatg
     9421 atttttcctt tcaggctatc ccaagaaaca ggcataccat tttgattggt caggttaaaa
     9481 tcaccggctt tatgccacac tgtatcagta acctttttgc ctttcctggt atctgtaacc
     9541 accgaatcag gaaaataatg ccggggcata tgaatggcac tctcactctt tgtctttaca
     9601 ataaaataag aaataagcgg tacaagcaac gcaatcatta cggcatataa agctgtttta
     9661 ttcacaatag tatatataaa agtaaaacca ttaaaccgct tccttgttca tccggcagcc
     9721 agttcaatgg ttttggtttc aaaaaaaaat ccgttattct ttgccagttg gtttttcaac
     9781 cggttttgct tcttctttat gatgcttggc cggcatggtc gtttcctgga aatgtttatc
     9841 gtaggtatta cgcaggttct tataagaatt accatcccag ataaaagccg caataaacca
     9901 gataaacagc aacaatggca ccacaatggt catgatcata ttcctgatct catcccccag
     9961 gtgcatgaat acagaaacaa tatagtaagc ttttgccagg gtaagtatac acaccattcc
    10021 tttaaacatc aataccagta gttcactcgg gttttcacct ttatgtatat tataaatgag
    10081 taaccctata gccagctcta taacagttat aacagataat agaatggtgg tctttctgac
    10141 ctttttctta aatgctgcat catcaatggt gtgatgatga atagtgactt cttcaaaagc
    10201 cggatgttgc ataagctgat aaattacaaa ttaataaatc acaaacctgc ctgcaggttc
    10261 caaactaaat tttatatcaa atagaaacaa aggaatacaa atacccaaac caggtccaca
    10321 aagtgccagt acaacccggc tttttcaatc atcaggtaat gacccctgtt ctggaataca
    10381 tttttctgtg tcatcatcag catgataata ttaatgatta cacctgaaaa tacgtggaat
    10441 ccatggaaac cggtgatgcc gtagaaatag cccccgaatg ccactggtcc ttttgaccgg
    10501 atatgaagtt catccagttt gatttctttg attaactgct gatctgtcat agcaactaat
    10561 tgctcatgac ttacagcatg attactttga tgtgccaggt ttaccaatgt ttcatgcggt
    10621 gttttttgca aggccgccat cgcagcttca ttacttacaa ggtctcccca tggatttgct
    10681 ctcgtggttt gcccgataat atcaatctgg ccatctatca gtactgcatg ttcaccggta
    10741 ataaggtgat gccactccca tgcctggcaa ccaaggaatg caagaccacc gataatggtc
    10801 cagaacaacc atttgatcac tccctgttta tcacctttat gtcctgcatg tactgccagc
    10861 accattgtta cggaactgat gatgaggata aaagtcatta cactcacaaa tgccaaaggc
    10921 agatttgcat gcccggcacc cggaaatgca ttgaacacca cgttaggatc cggccagaaa
    10981 ttctggctga aacgaataga tccgtaagag atcaggaatg caccaaatgt aaaggcatca
    11041 ctcatcagga aataccacat catcacttta ccatactcca ggttgaaggg actcttacct
    11101 ccgctccacc atttggtctg atgtactgtt gctgttgtcg ccatgtcagt tgtcaggttt
    11161 tacttgttgt ttattatcat cagttaccgc cgattaccag gaagaatacc agcaggtaaa
    11221 tccataaaaa atctacaaaa tgccagtatg ttgctgctac ttctacagga actgaactgt
    11281 ataatttaat ttttccaaaa aaagctttga tcagcataac catcaaagcc actattcccc
    11341 caataacatg caaagcatgt aaactgaata tcacatacag gaattgacca gccccggctc
    11401 ccctgaatgt tacctgctgc gcccataaat tctgaaaccc catccactgg aacactatga
    11461 ataccgaacc gagcaacact gttgccccga taagcattct gtatttgttc atttccctgt
    11521 tcttaaaaga tcttaatgcc agctgcaccg ccaggctgct caacaggatc actatagtgg
    11581 aaatccagaa cactttcggc aaggttaccg gcttccagtt tacctggtta ctttttacta
    11641 tgaatgcact cgtcaatcct gcaaacatca ttacaagact gccaatagcc acccacaatg
    11701 taaacttgtg gggatgcatt ttctgtttat gctgattact cacaatatcc atctctcttt
    11761 tatcttttaa taactatgct ttacttaata ataaggctaa caaaacgacc ggtaaataca
    11821 tataactacc aaacatcact tttcttgccg aactaacatc catattctta tacaaggcaa
    11881 cacatctccc gatcataaac aggttcgcca atattaccag tccgatactt attagcccca
    11941 ttgtgtcctc ataactgcac ataccggtaa aataaggcaa cagcgtaacg ggtaccagca
    12001 acagcgaata agcaattgtc tgaatggccg taaacctggt tggtccctca ttggctggta
    12061 acagcttaaa tccaacggct gaataatcct tgtgcgctac ccaggcaata gcccagaaat
    12121 ggggaaactg ccaaaagaac tggaaggcaa acaatatcca gccacctgtt gataattgat
    12181 catcgccggc tgcccagcca ataaggcagg gcaatgctcc gggaactgcc cccatcaata
    12241 cagctattga atttactttt ttcaacggtg tatagatgaa tgcatacagg aacaagctaa
    12301 aggcggctaa tcctgccgat aaccagttga aataatagcc aaggataaaa acacccaaag
    12361 ctcctgttac aatagcaaat gcccatcctt cccctacact cattcttcct gcagccacag
    12421 gccttgtgga agttcttttc atttgtgcat ctgtatcttt ctcaactacc tggttgatgg
    12481 cattggcact acctgttacc aacatcccac ctgcaaagag caagagtatc attatccagt
    12541 tgtattcaac aacttttggt gccagcaggt aactgatcac actactgaaa accaccatga
    12601 tactcagcga aggctttatt aacagtaaat aatctttgat cttcttcata tcagccttca
    12661 ctatattgtc aaaaaaactt caatctttca aaaaacacca atcaatccca ctatcaattt
    12721 agtgcttact ttcattagca ccaattggct ccgtttgcgg aataaaatcc cttccatctt
    12781 tagagtaatc atatggccag cgatgtactt ccggaatttc tccctcccag tttccatgac
    12841 ccggattgat ctttgttgtc cattccagtg tattggcacc ccaaggattg ggtgtggtca
    12901 cttttcttcc tttaaatatt gaatagaaga aattgaaaac aaacatcaac tgtactgcaa
    12961 atacaataat ggaaacaatg gtgatcattt tatccagttc accaaactgg ttgaaggaaa
    13021 cccatccact cttatcaaga taacgacggg ggataccagc aaggccctgg taatgcatgg
    13081 gccagaagat caggtaagca ccgatcatag ttacccagaa atgcatatac ccaagtgtgt
    13141 tatttaagta acgtccaaac attttcggga accaatgata cactccggca aacatgccga
    13201 agaaagaggc gactcccatt acaatgtgga agtgagccac tacaaacatg gtatcatgca
    13261 ggtgaatatc aatagtagag tttccaagga agatacccgt caaaccgccg gagataaata
    13321 agctgacaaa cccaatagca aataaggtgg ccggagtaaa ccggatatta cctctccata
    13381 aagtagtaag ccagttaaat acttt
//
LOCUS       DAORTI010000094        13289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4587_length_13289_cov_6.820387, whole genome shotgun sequence.
ACCESSION   DAORTI010000094 DAORTI010000000
VERSION     DAORTI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13289
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_13289_cov_6.820387"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLO70_11715"
     CDS             <1..785
                     /locus_tag="PLO70_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75179.1"
                     /translation="IGFHPPEQLNRIMKKTVLSLFVSSTLFLTGCLETTQEITLNEDG
                     SGTISNTNDMSALIGMAKQMGGADMEGANQVVDSTISMNAGADSIPNLTAEEKEMVKK
                     GTLRINMDMKNEKFLTKLSFPFTSPAEVAVYNKLSGKIMSETLKEQMGEGMPMGGEMP
                     EPSSFDDYYTINFANGLLTKTLDKEKFTRAADDEYLKSMKETAAMGLSMKANYVINLP
                     RPAKTVEGKGIKLSEDKKTITISSDLDDFFDDPSKLEYRIEY"
     gene            complement(861..1790)
                     /locus_tag="PLO70_11720"
     CDS             complement(861..1790)
                     /locus_tag="PLO70_11720"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQZ75180.1"
                     /translation="MKVHYDIEQLPEFRNAIITIGTFDGVHMGHRQIIDKLKAEAKAN
                     DGETVIITFHPHPRKVVSSAILGIRLINTLSERIEILTRLGINHLVVVPFTEVFANQP
                     AEDYIKNFLVDKFHPHTIIIGYDHRFGRDRLGDYRLLEKKATEYNYQLKEIPKHVLEN
                     IAISSTNIRQAILHNDIATADTLLGYEFFFSGVVVHGDKIGRKLGYPTANLKINDDEK
                     IIPGNGIYAVYAQPEGYTERLKGMMSIGFRPTVDGKKRVVEVNIFDFDTEIYGQTLKV
                     FVKKYLREEIKFNGLEELVQQMDQDKINSLKVL"
     gene            1879..3048
                     /locus_tag="PLO70_11725"
     CDS             1879..3048
                     /locus_tag="PLO70_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQZ75181.1"
                     /translation="MSLYSKRGVSAQKEEVHAATKNLDKGLYANAFCKIYPDVLCGDD
                     AWVNVMHADGAGTKSILAYLYWKETGDVSVWKGIAQDAIVMNLDDLLCIGIYDNLLFS
                     STIDRNKNVIPAEVLEAVITGTQEFFDTMKSFGVNISFMGGETADVGDVVRTIAVNGT
                     MTARWPKSKLVTNEKIKAGDVIVGLAGYGQSDYETQYNSGLASNGLTSARHDVLHKTY
                     GARFPESYDTSLDESVVYIGPHRMTDEVREGAIKYEVGKLLLSPTRTFAPVMKELLEN
                     HFEFIHGLIHCSGGGQTKCLKYVPENVRIVKDNLFEPPIIFKLIQEASKSDDREMYQV
                     FNMGTRLEIYTNEKEADAIIAVSKRFGVEAQVIGRVEKSVKKELVILNAGKEIVF"
     gene            3094..3783
                     /locus_tag="PLO70_11730"
     CDS             3094..3783
                     /locus_tag="PLO70_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQZ75182.1"
                     /translation="MSEPIIELKNVNIYQGDNLILQDVNLTVNKGEFVYLVGKTGTGK
                     SSLLKTLYGELELKEGEGTVAGFNLRGLDWKKVPYLRRNLGVVFQDFQLLTDRNVNDN
                     MKFVLRATGWTDEKLMNEKIADVLDKVGLRSKGFKMPFELSGGEQQRIDIARALLNSP
                     KLILADEPTGNLDPETSDEIMNLLFQISRDYNTTVVMATHDYIVVQKFPARMIRTERG
                     RVADNATISIE"
     gene            complement(3764..4888)
                     /locus_tag="PLO70_11735"
     CDS             complement(3764..4888)
                     /locus_tag="PLO70_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQZ75183.1"
                     /translation="MNRHLHIVCLDVPSPADYGGAIDMLYRIESLYNAGIKIHLHYFS
                     YNHRGNPNELNQYCESIHVYERKTGHKGFSFSKPYIVSSRINQELITKLNKDNHPILL
                     EGVHCTGILPQLNTGNRKVIVRLHNDEADYYKQLSSVEKNIFKKIYFRYESHLLSKYE
                     HSLPPQYLYACITTKDEAHFREQHGLKNSFFLPAYTPYEKINSSEGIGNFCLYHGNLS
                     VPENEKAATWLLTKVFSKIKVPFVIAGKNPSKRVDKLSHLYQHTCLVANPTDTEINDL
                     VKKAHINILPSFSNTGIKIKLLHALFEGRHCVTNEAMVNGTDLEAACHIGTNANAIAA
                     IIMQLHHQPFTTEEIRLRENLLGNTYNNALNTKQLIQYLW"
     gene            complement(4885..5976)
                     /locus_tag="PLO70_11740"
     CDS             complement(4885..5976)
                     /locus_tag="PLO70_11740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ75184.1"
                     /translation="MKRIYFTVTNDLIYDQRMHRICTTLAENGYDITLVGRKLPSSLP
                     LAEKKFKQERIRCFWIKGKFFYAEYNIRLFFFLLLKKMDAICAIDLDTIIPCLYISRC
                     KKITRIYDAHEFFSELKEVISRPGIQKIWQRIEKKAVPKFKWGYTVSESIAEEFHRRY
                     GVNYKTIRNVPLLQELDNMVTTEKFILYQGAVNQARGFEYLIPAMQWINARLVICGDG
                     NFMPQLKKLISDYQLQDKIELKGMLAPSALGKIAQLAYIGVAVPEKEGLNQYLALPNK
                     FFDYIHAGLPQVTVDYPEYRKMNKQFEVAVLIDNLAPKRIAAAINNLLVDDVLHRRLK
                     ENCLIARQELNWQQEERKLLSFYQSVLTY"
     gene            complement(6057..8690)
                     /locus_tag="PLO70_11745"
     CDS             complement(6057..8690)
                     /locus_tag="PLO70_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75185.1"
                     /translation="MRNIIIALSSLLITYISSAQAPLGAGNRPGAGRTQMTGSFYGKL
                     IEAKSLKPIEYASVQLLQNRFDSVSKKRKEVVVAGMLTKHNGEFRLENIPVFGQFKLQ
                     VSIVGFKPYEQTVSFDMKPSGTNNGDMTAMMSTLDKDLGNIKIDIEDKVLENVTVTSE
                     KPSLQLGIDRKVFNVDKNIVSAGGTAVDIMRNVPSLNVDIDGNVSLRNNAPQIFVDGR
                     PTTMELDQIPADAIESVEIITNPSAKFDASGGTAGILNIVLKKNRKVGYNGSLRTNID
                     SRARIGLGGDINVRQNKINVFMSGNFNQRRSISTGTTERLSLIENPNNFLFQDDRSEF
                     DGNFLFGRAGIDYFMTNRSTLSASVNLGSGKFNPINISNLLTDTLSSPVVTSLTKRYS
                     DMSREFNNLGASLNFKHNFPKAGRELTADINYRGRTGPNDNLITTEFYNPDLTTIKNI
                     QRQRQIGDGDNTSIILQGDFVNPLSDKSKLELGVRAAFNENTSTNAFYTVDPVTGTLT
                     LQPASQVDYVSNDRVLAAYTTFSNRINNFGYQLGLRVESSNYQGELTNTNEKFDIDFP
                     VSLFPSAFLSQKLKNEQELQLNYSRRINRPNFWQLSPFTDSTDFLNLSKGNPGLNPEF
                     TNSLELSYQKIFKNRDNLLLSVYYKNTNDLITRYQEIQTEPGTGKDVIINTFINANSS
                     YVTGLEMTLKNNITKWWELTSNFNLFTSKIDIDIPSIPEQDQFASWFVKINNNFKASK
                     NLSFQLSGSYQSKSILPPGGSGGGGGRGGGMFGQSTSAQGYVKPVYYVDAAIRYNFLK
                     DNRASLSVNMSDIFRTRVSDIHSESPFFIQDVFRRRDPQILRINFNWRFGKFDASLFK
                     RKSNKGQEDQNGMDGMNMRPQ"
     gene            complement(8817..9422)
                     /locus_tag="PLO70_11750"
     CDS             complement(8817..9422)
                     /locus_tag="PLO70_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HQZ75186.1"
                     /translation="MNYWIFIIPVISAFIGWVTNWVAIKMLFHPREPKKILGITIQGI
                     FPKRQQQFAEKLGKLVSDEFLSFEDIEQKISSPDNLKKIMPMIENHIDDFLRNRLSVE
                     MPVISMFIGDKTITKLKSAFMTEIELLFPIVMKQYAANLKTELDLEHIVIQKVAGFSS
                     DKLEEVLYQIMSKEFRFVEIIGAVIGFIIGLVQILITQLTM"
     gene            complement(9469..9547)
                     /locus_tag="PLO70_11755"
     tRNA            complement(9469..9547)
                     /locus_tag="PLO70_11755"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9510..9512),aa:His,seq:gtg)
     gene            9805..11955
                     /locus_tag="PLO70_11760"
     CDS             9805..11955
                     /locus_tag="PLO70_11760"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQZ75187.1"
                     /translation="MKRLPIVILMVVAASFLAFNSMGKSTRSLPPGNYEKIMKLVGEM
                     LSQGHYSPQSINDAFSKKVFKKFVTDLDPDKNIFLQSDIDALKKFETKVDDEVKGAIV
                     EFPLAAGKIFNKRVEESSVLYNEILSSAFDFTVNEEVLLDADQVGFTVNATEQKERWR
                     KKLKFMTLERYADLLESREKNKGKEGFVVKTDEELEKDARERVKKIMDRTFERYRFKF
                     NDDDKFNMYVNAITTTMDPHTEFFPPVDKRYFDEEMSGRFFGIGASLQYEDGNIKIAS
                     VLTGSPAYKSGELQAGDIIQKVAQGKEEPVDLTGYLVTDAVKVIRGKKGTEVRLTVKK
                     LDGTVKIVSLVRDEIVQDESFARSAIVNEGGSKVGYIFLPEFYADFDRPNGNRSYIDV
                     AKEVTKLKEEKVDGIVIDLRNNGGGSLYDVVQMAGLFIEDGPIVQVKDRDNKPSVLKD
                     KDRNVLYSGPLAVMVNEFSASASEIFAAAIQDYGRGIVIGSTSTYGKGTVQRNIGLDP
                     ENGFSMSNSELGTIKLTLQKFYRINGGSTQLKGVTSDIILPDNLEYLKVREKDSEDAL
                     PWDEISKATYNTWNAGYSLETIKQLSNMRLENDPTFRLIGETTEWLSRQSEKKYSLQL
                     EKYRKDQEKIRSSVKQLESLLKLKGQLDVTALPAEQNRWAEDKPKQERFQQWLKSLQK
                     DIYLDQALKVMNDMIGQQNLVKSKTTEEPVKKAF"
     gene            complement(12016..12525)
                     /locus_tag="PLO70_11765"
     CDS             complement(12016..12525)
                     /locus_tag="PLO70_11765"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQZ75188.1"
                     /translation="MSRPDLTRVPEWYHRYIAQVEGDDLILLLNEQLTSFLFFLENLD
                     AEKREYRYGPDKWTIKEVLQHILDAERVFAYRALCIARGEKQPLPGFDENNYVTHSKA
                     DKREWKDMLEEFKAVRYSTTMLFASFDEEQLEATGTASGWTVYVRGIGFIIIGHLNHH
                     LHIIKERYL"
     gene            complement(12533..13039)
                     /locus_tag="PLO70_11770"
     CDS             complement(12533..13039)
                     /locus_tag="PLO70_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75189.1"
                     /translation="MKYFIYLPALLLLAATTGCGNASDEESTTTPANTSTMQPATIST
                     GGDTARTQTVQVQPAADLSTQVNSTNQPPAPVISKAGLNPAHGQPGHRCDIQVGAPLD
                     SKPTQATVNTNPQPATITSTPSPAAPVLNTNPVPGATAAGLNPAHGQPGHRCDIAVGA
                     PLNSKPTQ"
BASE COUNT         3935 a         2511 c         2872 g         3971 t
ORIGIN      
        1 aaattggttt tcatcctcct gaacaattaa atagaattat gaaaaaaaca gtattgtcat
       61 tatttgtttc cagcacctta tttcttaccg gttgtctcga gaccacacag gaaattaccc
      121 tgaatgagga tggcagcgga actatcagca atacaaatga catgagtgct cttattggta
      181 tggctaagca aatgggcggc gctgatatgg aaggcgcaaa ccaggtagtt gattcaacta
      241 tttccatgaa tgcaggtgct gatagtatcc caaaccttac agccgaagaa aaggagatgg
      301 ttaaaaaagg tactctccgg attaatatgg atatgaaaaa tgaaaaattc ctgaccaaac
      361 tttcttttcc ttttacctct cctgcagaag tagcggtcta taataaactt tccgggaaga
      421 taatgtctga aacattaaag gaacaaatgg gagaaggtat gccgatggga ggcgaaatgc
      481 cggagccaag ttcttttgat gattactata ccattaattt tgccaacgga ttgctcacaa
      541 aaactttgga caaagagaaa tttaccaggg ctgctgatga tgaatacctg aaaagtatga
      601 aagagacggc tgcgatgggc ttgtcaatga aagcaaatta tgtgattaac ctgccccggc
      661 cggcaaaaac ggtggaaggt aaaggtatca agctctcaga agacaaaaaa acaattacta
      721 tctcgtctga tttagatgat ttctttgatg atccctccaa actggaatat agaatagagt
      781 attaaggcaa cagcgtataa actatttttt aaaggtaaat gtttaagagt ctttaccatt
      841 ttgattttac tgatcattgc ttataacact ttcaaactat tgattttgtc ctggtccatc
      901 tgttgtacca gttcttccag tccgttgaat tttatttctt cccgcaggta ttttttgaca
      961 aacactttta aggtctggcc atagatctct gtgtcgaaat caaaaatgtt tacttctact
     1021 acccgtttct ttccgtccac cgtgggtctg aagccgatgc tcatcatacc tttaagtctt
     1081 tctgtatatc cttcaggttg ggcatataca gcatagatcc cattcccggg aattattttc
     1141 tcatcatcgt ttattttcag gttggcggtg ggatagccca attttctccc gatcttatct
     1201 ccatgtacta caactccgga aaagaaaaat tcataaccaa gaagggtgtc cgcagtggca
     1261 atatcattgt gtaatatcgc ctgccgtata ttcgtggagc tgatcgctat attttccaat
     1321 acatgttttg gtatctcctt taactggtaa ttatattccg ttgctttctt ttccaataag
     1381 cggtaatcac ccagcctgtc acgaccgaac cggtggtcat atccaatgat aatcgtatgg
     1441 ggatgaaatt tatctaccag gaaattcttg atataatctt ctgccggctg gttggcaaaa
     1501 acctctgtaa aagggactac aaccaggtgg ttgataccca atcgggtaag gatctcaatt
     1561 ctttcactca gggtattgat cagccggata cccaggatgg cagaagagac cactttacgg
     1621 ggatgtgggt gaaaggtgat gatgacagtt tctccatcat tggcttttgc ctctgctttt
     1681 agtttgtcaa ttatctggcg gtgacccatg tgcactccgt caaaagtgcc aatggtgatg
     1741 atggcattcc tgaattcggg taattgttca atgtcgtaat gtactttcat aaagagctgc
     1801 gaagataaat attagtcatt agtcaggagc tgcgggtctt tagcccggaa tagttagttt
     1861 tgcggctcaa atctcactat gagcctgtat tcaaaaagag gagtttctgc ccaaaaagaa
     1921 gaagtgcatg cagctaccaa aaacctggac aagggtcttt atgccaatgc tttttgcaaa
     1981 atataccccg atgtattgtg tggagacgat gcctgggtga atgtgatgca tgcagatggt
     2041 gccggcacta aaagtatact ggcttattta tactggaaag aaactggtga tgtttctgta
     2101 tggaaaggta tagcacagga tgccatcgtc atgaatctgg atgatctgtt atgcatagga
     2161 atttatgata acctgctttt ttcttccacg attgaccgga acaagaatgt gattcctgct
     2221 gaggtactcg aagcagtgat taccggaacc caggaatttt ttgataccat gaagagcttt
     2281 ggcgttaata tcagtttcat ggggggtgaa acggctgatg tgggcgatgt ggtaagaacg
     2341 attgcagtga acggaacaat gactgcccgc tggcctaaat caaaactggt taccaacgaa
     2401 aaaataaaag caggtgatgt gattgtggga ttagccggtt acggacagtc tgattatgaa
     2461 acgcaataca acagcggact ggccagcaac ggtcttacca gtgccaggca tgatgtgttg
     2521 cataagacgt atggggcaag attcccggag agttatgata cttcgttgga tgaatcggtt
     2581 gtttacattg gcccgcacag aatgactgat gaagtgaggg aaggagctat aaagtatgaa
     2641 gttggaaaat tattactctc gccaacaagg acatttgcac cagtgatgaa agaattgctg
     2701 gagaatcatt ttgagttcat acacggactc atccattgca gtggcggcgg gcaaaccaaa
     2761 tgcctgaaat atgtaccgga aaatgtccgg attgtaaagg acaatttgtt tgagcctccc
     2821 attattttca aattgataca ggaagcaagc aaatctgacg acagggaaat gtaccaggtg
     2881 tttaatatgg gtaccaggtt agagatttat accaacgaga aagaggccga tgctattatc
     2941 gctgtttcaa aaagatttgg tgtggaggca caggttatag gcagggtgga gaaaagtgta
     3001 aagaaggagt tggtaatttt gaatgctggg aaggagatag ttttctgatt ttattaaccc
     3061 atattgcttt attttgcaac aaacccataa atgatgtccg aaccaatcat tgaattaaag
     3121 aatgtaaata tttaccaggg tgataacctt attttacagg atgtaaacct gaccgtgaac
     3181 aagggagagt ttgtttacct ggtaggaaag accggaaccg gtaagtcaag tcttttaaag
     3241 acattatatg gtgaactgga attaaaggaa ggagagggca cagtggccgg gtttaacctg
     3301 cggggccttg actggaaaaa agttccttac ctgcgccgca atctaggagt ggtgttccag
     3361 gattttcaac tgctgacaga ccgtaatgtg aatgacaaca tgaaattcgt tctgagagct
     3421 accggctgga cagatgaaaa actgatgaat gaaaagatag cagatgtgct ggataaagta
     3481 gggctacgtt caaaagggtt taagatgcct tttgagctga gtggcggaga gcagcaacgt
     3541 attgatatcg caagggcctt attaaattct ccgaaactta tactggcaga tgagcctacc
     3601 ggaaatctag atcctgaaac ttcagatgaa attatgaacc tgctattcca gatttcaagg
     3661 gattacaaca ctactgtagt aatggctaca catgattata ttgtggtaca aaaattccct
     3721 gcccgtatga ttcggactga gcggggaaga gtggcagaca atgctaccat aagtattgaa
     3781 taagctgttt tgtattcaac gcattgttat aagtattgcc cagcaaattt tctctcaacc
     3841 tgatttcttc tgttgtaaaa ggctgatgat gtagttgcat gataatagct gcaatggcat
     3901 tggcattggt accgatgtgg caagccgctt caagatcagt gccattaacc attgcttcat
     3961 tggtaacaca gtgccggcct tcaaacaggg catgcagcag ttttatcttg atgcccgtat
     4021 tgctgaaaga gggtagaata ttaatgtggg ctttttttac gaggtcattt atttcggtat
     4081 ctgtcgggtt tgccaccagg caggtatgct ggtacagatg agacagttta tctacccgtt
     4141 ttgaaggatt ttttcctgca atgacaaagg gcacttttat tttagaaaaa accttggtca
     4201 gtaaccaggt ggctgctttt tcattttcag gtactgaaag attgccatga taaaggcaaa
     4261 aattaccaat accttctgaa gagtttatct tttcatacgg agtataggcg ggaagaaaga
     4321 aactattttt caatccatgc tgttccctga aatgagcttc atcttttgtt gtaatacagg
     4381 catagaggta ctgcggtggc agtgaatgtt catacttact cagcaagtgg ctctcgtaac
     4441 ggaaatatat ttttttaaat atattttttt caacggatga taattgtttg taataatcgg
     4501 cttcatcatt atgtaaacgt actatcactt ttctattacc ggtattgagc tgcggtagta
     4561 tacctgtaca atgcacccct tccaataaga tggggtgatt gtctttattc agttttgtga
     4621 tcaattcttg atttatcctg gaagaaacga tatagggttt tgagaatgaa aagcctttat
     4681 gtcctgtttt tctttcgtac acatgtattg actcacaata ctggttcaac tcattgggat
     4741 tgccacggtg attataagag aaataatgga gatgaatctt aattccggca ttatacagcg
     4801 actcgattct gtaaagcata tcaatggcac cgccgtaatc ggcaggtgag ggtacatcaa
     4861 ggcatactat atgtaaatgt ctgttcaata tgttaaaact gattgataaa aggagagcag
     4921 ttttctttct tcctgctgcc agtttaattc ctgtcttgct atcaggcagt tttctttcag
     4981 tctcctatgt aaaacatcgt cgaccagtaa attgttgata gcagcagcaa ttcttttagg
     5041 ggcaaggtta tcaattaaaa cggcaacttc aaattgtttg ttcattttcc ggtattcagg
     5101 gtagtccact gttacctgtg gcaatccagc atgtatgtaa tcaaaaaatt tattgggcaa
     5161 ggcaaggtat tggttcaggc cttctttttc gggtacagcc actcctatat acgccagttg
     5221 agcgattttt cctaaagcgg acggtgctaa catacctttc agttcaattt tatcttgcag
     5281 ctgatagtca ctgatcagtt tctttaactg gggcataaaa ttaccatcgc cacagattac
     5341 cagcctggca ttgatccatt gcatggccgg aataaggtat tcaaatcccc tggcctggtt
     5401 cactgctccc tggtaaagga tgaatttttc tgtagttacc atgttatcca gttcctgcaa
     5461 taaaggcaca ttacggatag tcttgtagtt tactccatat cttcggtgaa attcttctgc
     5521 aatactttca cttacggtat atccccattt gaattttggc actgctttct tttctatacg
     5581 ctgccatatt ttttgtatgc ccggccggct gatcacttcc tttaattcag aaaaaaattc
     5641 gtgggcatca tagatacggg taattttctt acacctggaa atatacaggc agggaattat
     5701 cgtgtcaagg tctatagcac aaatggcatc catcttcttc aatagcaaaa agaagaacaa
     5761 gcggatatta tattcagcat aaaaaaattt cccttttatc caaaagcagc ggatacgttc
     5821 ctgtttaaat tttttttctg ccagtgggag tgaggaaggc agttttcttc caaccagggt
     5881 tatgtcatag ccgttttctg ccagcgtcgt gcagatacgg tgcattcgct ggtcataaat
     5941 aaggtcgttg gtaacagtaa aatagatccg tttcaaataa agacttaata atggttgcca
     6001 gaaaagaaaa ggttttgcaa atatgcaaaa ccttacactc ttaatctata aattttctat
     6061 tgtggtctca tgttcatgcc atccatcccg ttttgatctt cctggccttt attactcttt
     6121 cttttgaaga gagatgcatc aaactttcca aagcgccaat tgaaatttat acgcaatatc
     6181 tgcgggtcac ggcgacggaa cacatcctgt atgaaaaaag gagattcaga gtgaatatcc
     6241 gataccctag tcctgaagat atcactcatg ttgacagata aggaggccct gttatctttt
     6301 aagaaattat aacggatggc ggcatcaacg taataaactg gtttaacata cccctgtgcg
     6361 gaagtacttt gtccaaacat tccaccacca cgtccaccgc ctccaccact tccaccggga
     6421 ggcagaatag attttgactg gtagcttcca gataactgaa agctcaggtt cttggatgcc
     6481 ttaaaattat tattgatctt aacaaaccag ctggcaaact ggtcctgttc tggtatggaa
     6541 gggatatcga tgtcgatctt tgatgtaaac aggttaaaat tgctggtaag ctcccaccat
     6601 ttggtgatat tattttttaa ggtcatttcc aagcctgtta cgtaactgga attagcgttg
     6661 ataaacgtat taatgatcac gtcttttcct gttcctggct ctgtttgaat ttcctggtaa
     6721 cgggtaatca ggtcattggt atttttataa tacacagaaa gcaataaatt atccctgttc
     6781 ttaaaaattt tctggtagga taattccagt gaattggtga attcaggatt caacccggga
     6841 tttcctttac tcaggttcaa gaaatcggtt gaatcagtaa atggagacaa ctgccaaaaa
     6901 ttaggacggt taattctcct ggaataattt aattgcaact cctgttcatt ttttaatttc
     6961 tggctgagga aagcactggg gaataaactt accgggaaat ctatatcaaa tttttcattg
     7021 gtgttggtaa gttcaccctg gtagttagaa ctttcaacac gaagtcccaa ctggtatcca
     7081 aaattattga tccggttact aaaagtagta tatgctgcca gcaccctgtc attactgaca
     7141 tagtccacct gtgaagctgg ctgaagggtt aatgtacctg tcaccggatc aacagtatag
     7201 aaagcattgg tacttgtgtt ttcattaaaa gcagccctta cacccaattc aagttttgac
     7261 ttatcactga gagggtttac aaaatcacct tgcaaaatga tactagtgtt atccccgtca
     7321 cctatttgtc tttgtcgttg aatatttttt atggtagtca gatcgggatt gtaaaattca
     7381 gtagtgatta aattatcatt tggtccggtt ctgcccctgt aattaatgtc agcagttagt
     7441 tcccggcctg cttttggaaa attatgtttg aaattcaatg aagcgccaag attattgaac
     7501 tccctgctca tatctgaata acgtttggta agtgaggtta caacagggga gcttaatgta
     7561 tcagtcagca ggttactaat atttatcgga ttaaactttc cacttcccag gtttacactt
     7621 gcagaaaggg ttgaacggtt ggtcatgaag taatcaatac ctgcacgacc aaacaaaaag
     7681 ttgccgtcaa attctgacct gtcatcctgg aataaaaaat tattcgggtt ttcaatcagg
     7741 gataaccttt ccgtagttcc ggtgcttatt gatctgcgct gattaaaatt tccactcatg
     7801 aaaacattga tcttattctg tcttacattg atatcgccac ctaaaccaat ccttgcccgg
     7861 ctatcaatat ttgttctgag gcttccattg tatcccactt ttctgttttt cttcaataca
     7921 atattcagta taccggctgt accaccggag gcgtcaaatt tagcggaggg gtttgtgatg
     7981 atctcaacac tttcaatagc atccgcaggg atttgatcta gttccatggt ggttggacgg
     8041 ccatctacaa atatttgcgg tgcattatta cgaagggaaa catttccatc gatatccaca
     8101 ttaagagagg ggacatttct catgatatca acagcagtac caccagcgga aacaatattc
     8161 ttatctacat taaatacttt tctgtcaata cccagttgga ggcttggctt ttcagaagtt
     8221 acggttacat tttcaagtac tttatcttca atatctattt taatattacc aaggtcttta
     8281 tccaatgtac tcatcatggc agtcatatca ccgttattgg taccactggg tttcatatcg
     8341 aatgaaacag tctgttcata aggtttaaaa ccaactatac ttacttgtaa tttgaactgg
     8401 ccaaaaacag gaatattttc aagacggaac tcaccattat gctttgttag cataccagct
     8461 actacaactt cttttcgttt ttttgagacc gaatcaaacc tgttttgcaa taactgtacg
     8521 gaggcgtatt cgataggctt taaagatttg gcttcaatta gctttccgta aaaggaacct
     8581 gtcatttggg tccggcccgc acccggcctg ttgccagcac ccaaaggcgc ctgggcggaa
     8641 gatatataag tgattaataa tgaggacaat gcaataataa tatttctcat ttctctgatt
     8701 tgtttattag acgtgtaaag gtgtccgaac ttgctgacgt tcttatgaca acttgatgaa
     8761 tggcagtatg acaaccatga aggggaaaag ttcggtttcc aacccttttg agataattac
     8821 atagtaagct gggtgattaa tatttgtacc aggccaatga tgaaaccaat gacagcgcca
     8881 atgatctcaa caaacctgaa ttcttttgac atgatctggt aaaggacttc ttccagtttg
     8941 tcggatgaaa aaccggccac tttctggata acaatatgtt caagatccaa ttcagttttt
     9001 agattagcag catattgttt cataacaatt gggaacagca attcaatctc ggtcatgaac
     9061 gcacttttca gtttggtgat tgttttatcg ccaatgaaca tactgataac aggcatttca
     9121 acgcttaacc tgttgcggag aaaatcatca atatgatttt ctatcatcgg cataattttt
     9181 ttcaggttat ccgggctgct gatcttttgt tcaatgtctt caaaggaaag gaactcgtcg
     9241 ctgacaagtt tccctaattt ttcagcaaat tgttgttgcc tttttgggaa aatgccctgt
     9301 atcgtgatgc ccaatatctt tttgggttcc cttgggtgaa agagcatttt aatggctacc
     9361 cagtttgtaa cccagccaat aaaggcagaa ataacaggaa taataaatat ccaatagttc
     9421 atacaaaaat tataaaatga aaccccgttt cgtttgaaac ggggtttttg ggggtggcta
     9481 accgggctcg aaccggcgac cctcagaacc acaatctgat gctctaacca actgagctat
     9541 aaccaccatt ttattcgcct ttccggcaga taccgggata aacaacagcg aattatgcgg
     9601 ctgcaaaaat aagaaaaatg aatgctctca aaaaataaaa gaacgtattc tttcgataat
     9661 cccaatcctg gtagcgaaag attactatta atcttattaa accacttatc aaaaaccatt
     9721 gttaaaaggc tgtaaaagca agaaaaacag gctttttgcg ttatttttga taacatgtcc
     9781 aatatattta aatacctgat gaatatgaaa agactcccta ttgtgatttt gatggtggtg
     9841 gcagcttctt tcctggcttt taattccatg ggaaaaagta cccgttccct tcctcccggt
     9901 aactatgaga aaataatgaa actggtaggg gaaatgcttt cccagggtca ctacagtcca
     9961 caatcaatta atgatgcctt ttccaaaaaa gtgttcaaaa aatttgtcac tgacctggat
    10021 ccggataaaa atattttcct ccaaagcgat atagatgcac tgaaaaaatt tgaaaccaaa
    10081 gtggatgatg aagtaaaagg ggctattgtt gaattccctt tggctgccgg aaagattttc
    10141 aacaaaagag tagaagagtc ttccgttctg tacaatgaaa tattatcatc ggcatttgat
    10201 tttacggtga atgaagaggt gttgcttgat gccgaccagg tcggatttac agtcaatgca
    10261 actgaacaaa aggaaagatg gagaaagaag ctgaagttta tgacattgga acgttatgct
    10321 gacctgctgg aaagccggga aaagaataaa gggaaagaag gctttgtggt aaaaaccgat
    10381 gaggaactgg aaaaagatgc ccgtgaaaga gtgaaaaaaa tcatggacag aacttttgaa
    10441 cgttaccgtt tcaaattcaa tgatgatgat aaattcaata tgtatgtaaa tgccattact
    10501 acgacgatgg atccgcatac tgaattcttt cccccggttg ataaaagata ttttgatgag
    10561 gaaatgagtg gccgtttctt tggtatcggt gcttccctcc agtatgaaga tggtaatatt
    10621 aagattgcca gcgtacttac aggaagtcct gcctataaat caggagaatt gcaggcaggc
    10681 gatattattc agaaagtggc acagggtaag gaagaaccgg ttgacctgac cggttatctt
    10741 gttacagatg cggtaaaagt gatccgtggt aagaaaggaa cagaagtacg gttaacagtt
    10801 aagaaattag atggtactgt taaaatagtg tcgcttgtcc gtgatgagat cgtacaggac
    10861 gaatcatttg cacgcagtgc gattgtaaat gaaggcggtt caaaagtggg ttatatattc
    10921 ctgcctgaat tttatgcaga ctttgaccgt cctaatggta accgcagcta cattgatgtg
    10981 gccaaagaag taactaaact aaaagaagaa aaagtagatg gtattgttat cgacctgcgc
    11041 aataatgggg gaggttcttt atatgatgtg gtgcagatgg ccggcttatt tatagaagat
    11101 ggccctatcg tgcaggtaaa ggaccgggac aataaaccaa gtgtattaaa agataaggac
    11161 agaaatgtat tgtattcagg tccgctggca gtaatggtaa atgaattcag cgcttcggcg
    11221 tctgaaatat ttgcggcggc aatacaggat tatggccgcg gtattgttat cggaagtacg
    11281 tctacttatg gaaaaggtac cgtgcaaaga aatatcgggc tggatccgga aaatggtttt
    11341 tccatgtcta actcagaact gggaactata aaacttaccc tgcagaaatt ttaccgtatc
    11401 aatggaggtt ccacccaatt gaaaggagtt acttcagata ttatattacc ggataatctt
    11461 gaatacctta aagtgagaga aaaagacagc gaggatgcgt tgccatggga tgagatttcc
    11521 aaagcgactt acaatacatg gaatgccggg tatagtcttg aaaccatcaa gcaattaagc
    11581 aatatgcgtt tggagaatga tcccactttc cggttaattg gtgaaacaac agaatggtta
    11641 tccaggcaaa gtgaaaagaa atattccctg caactggaaa aataccgtaa ggaccaggaa
    11701 aagatcaggt ccagtgttaa acaactggag tcattattga aactgaaagg acaactggat
    11761 gttactgcat tacctgcaga acaaaaccgc tgggcagaag ataaaccaaa gcaggaacgt
    11821 tttcagcaat ggctgaagag cctgcaaaaa gatatttatc ttgaccaggc cctaaaagtg
    11881 atgaatgata tgattggtca gcaaaacctg gtgaaaagta aaacaactga agaacctgtg
    11941 aagaaagcat tttaaaaata tttagtaata aaaaaggcga tgcagcaagg catcgccttt
    12001 tttattacat gttgtttaca ggtatcgctc tttaataata tgcaggtgat gatttaaatg
    12061 accaattatg ataaatccta tgccccgtac atagacagtc catccgctgg cggttccggt
    12121 ggcttccagt tgttcctcat caaatgaggc aaacagcatg gtagtagaat agcgaactgc
    12181 tttaaattct tccagcatat ccttccactc tcttttatca gcttttgaat gtgtaacata
    12241 gttgttctca tcaaaacccg gaagaggctg tttctctcct cgggcaatac aaagggcccg
    12301 gtaggcaaat accctttccg catcaagaat atgctgcagt acttctttta tagtccactt
    12361 gtccggacca taacgatact ctctcttttc cgcatcaaga ttttcaagga aaaataagaa
    12421 agaggtcaat tgctcattca aaagcaagat cagatcatca ccttcaacct gggcaatata
    12481 acgatggtac cattccggaa ccctggtcag atcggggcgg gacatataat tattattgag
    12541 tcggtttact attaagcggg gcgccaactg caatatcaca tcggtggcca ggttgaccat
    12601 gtgccgggtt tagtcctgcc gctgttgctc cgggaactgg atttgtattc agaacaggtg
    12661 cggccggtga aggagtagat gttatcgttg ccggctgagg attcgtatta actgtggctt
    12721 gtgttggttt gctgtctaat ggggcaccca cttgtatatc acaacgatga ccaggctgtc
    12781 cgtgtgcagg attgaggcca gctttgctga taacaggtgc aggaggttgg ttcgtgctat
    12841 tcacttgtgt tgacagatct gctgccggct gaacctgcac tgtctgtgtc cttgctgtat
    12901 ctccaccggt agaaattgtc gccggttgca ttgttgaagt gttagccgga gtagttgtgc
    12961 tctcttcatc tgatgcgttg ccgcagcctg ttgtggcggc cagtaataac aaagcaggca
    13021 aataaatgaa atatttcatg tgttgtattt tataataaat gacatgcaaa ataggtaagt
    13081 tttgtgaaag cagggttgta atattcctgc tattgaagat acagtcagca aagtgcagta
    13141 caggatgagt ctcaaggatt ttccattaaa atgtatcccg gttggtaatg gtatcaatac
    13201 taacctggac tcagctgttc aataatttat ttctactatt ttcttttgct gcaatttttg
    13261 catttgtacg ggtactatat ttataattt
//
LOCUS       DAORTI010000095        13253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4614_length_13253_cov_6.689120, whole genome shotgun sequence.
ACCESSION   DAORTI010000095 DAORTI010000000
VERSION     DAORTI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_13253_cov_6.689120"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLO70_11775"
     CDS             <1..820
                     /locus_tag="PLO70_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ75190.1"
                     /translation="AYGARIYDAQIGRWNHIDPLSEKMRRHSPYNYTFNNPIRFIDPD
                     GMKPDDIIRVNSQGYITKVEKADGPHKVMLGNQELKFIDQNFDQKQFQAMLPEKSVRY
                     DFDYSENSTRIFTPYSSKEMSTNFNDLEIGEIRDKFQSKDNANPQAPGALLSVGLYLL
                     KLGHNDFDFADDMTSLTKDGGNVNIGPPIQSKFAKDGTQGFVKFEHTNDLYNVYDAGN
                     FMTGKAFSMIGLSEKRILDGADTNSRITFNGPDTKADQKALSNGVKYPWVIWSK"
     gene            1261..2166
                     /locus_tag="PLO70_11780"
     CDS             1261..2166
                     /locus_tag="PLO70_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ75191.1"
                     /translation="MAGVSSKALAFGNPQNKFKFNKGSELQNKEFSDGSGLELYATNF
                     RSLDPQLGRFWQTDPKHDCSQSLYSAMNNNPILYNDPLGDTIRISFKTGFLGIFGRKA
                     NLTYDEKNQRWNNKDGSAYTGKTTKFSNMVLGDLKTNQKNDLGNAIVSNLATDILDHS
                     IMRGDPNSNTINDPNIYYSGSVSDNQKILQGGKSNSSPSYVVLGHEMAHKFSRNLGIR
                     NLPWFGTPPDQRGVDEYNAMYYENVLRQANGLPLRTAYQEANGITQGVILNAAGNLQS
                     LPPNLASQNMNLPLYQTLRTFLRLF"
     gene            2177..2650
                     /locus_tag="PLO70_11785"
     CDS             2177..2650
                     /locus_tag="PLO70_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75192.1"
                     /translation="MRLSILGLLIFISCNAPKKIIHIKDLTLTLTKKECGSYEVKDNE
                     ELIGYVVKPFYIEVEKKENHIVQFEKLIKANYSDYYDRFYRTYWFSKNSSSDTLILVA
                     MLSTKQLSSIPNWQCEEQEADTYNLLYKNKSNNKHPRFFTYNCTKGRFKILGDPD"
     gene            complement(2720..4033)
                     /gene="creD"
                     /locus_tag="PLO70_11790"
     CDS             complement(2720..4033)
                     /gene="creD"
                     /locus_tag="PLO70_11790"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HQZ75193.1"
                     /translation="METLESIKSATPFWGKGKLFLKCIIIFLMAMLLWIPTNFIMEVV
                     KEREGRQKEAIADISSKWAGRQAVTGPLLMIPYKLLLVDEKGSQSWQRQNAYFMPDML
                     DVSSKVFPEKRKRGIYEVAVYKTDIMLTGRFNNIPWQKLEIAADQILWNEAVLLFKVQ
                     DQLKGINEDLFVKWNDSRFVFTTQATGQTPLEDAFFTSVPITQDNAASGSTFSIHFSL
                     NGSEQLLFSALARENKIEMSSVWPDPGFTGIKLPDSRQVTDSGFTAKWKYMNRAMPQV
                     WKNTFYNFSAAQFGADLLIPVDSYNKTERSVKYALLCIMLTFAAFFLVETIYKRPLHL
                     VQYGLAGLALVLFYTLLLSISEYTGFNLAYLIAGSATIGLVGWYVGSILKSSKLALFI
                     SFVLAVVYSYIFSIIQLQDYALLMGSIGLFVALGIIMYFSRKLEW"
     gene            complement(4020..4457)
                     /locus_tag="PLO70_11795"
     CDS             complement(4020..4457)
                     /locus_tag="PLO70_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75194.1"
                     /translation="MNYFLYTFYTWILSLLVLVVGLAGYDFYLFAAGQSFDFFFPEFI
                     ALIFFLMVASSPAFLMAWGVLSIIIMSAYSQVEKIGLWILAVILSIVINIICLLLVFA
                     REEISREMLWMCWPAYLSAAVVITIRSKQFFSLIHKSKNDGNT"
     gene            complement(4518..4817)
                     /locus_tag="PLO70_11800"
     CDS             complement(4518..4817)
                     /locus_tag="PLO70_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQZ75195.1"
                     /translation="MKNPITGLSKVFESRIRLGVMSVLMVNEEVNFNDLKEMLEVTDG
                     NLATHLVNLEENGFIKVHKGFIGKKTNTTYSVTKSGEKAFTDHIIALENMIKGMK"
     gene            complement(4849..5490)
                     /locus_tag="PLO70_11805"
     CDS             complement(4849..5490)
                     /locus_tag="PLO70_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75196.1"
                     /translation="MNETSQQTDSLQDIKDIRRIMDRSSRFISLSGLSGIAAGVCALA
                     GAVVANTILNNYYGSYNSQGFFSGDDFSRLKIRLAGLAAVVFAMAFASSFYLTWKKAR
                     KQGLPVWDYTSKRLAWNMIVPLVTGAAFIAGMLRYDIWIFVAPACLLFYGLALVNASK
                     YTLSDIRYLGYCEIVLGLVNMFFIGYGLWFWAIGFGVLHIIYGIIMWSKYERK"
     gene            5628..8102
                     /locus_tag="PLO70_11810"
     CDS             5628..8102
                     /locus_tag="PLO70_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HQZ75197.1"
                     /translation="MKQYLILFLLNMLVAALPVLSQTKTIRGTVKDSHSEEPIPFASV
                     SFKNTTLGKLTDSSGSFTFYFNKWPSDTLEITCVGYQPFRLVLATSNDSILTTILMER
                     GTFNEGAQLKVKVNKGLLVWRKIVQNKPRNDRYRFDNFSYELYNKLELDLKNINFEKF
                     GKFKPFKPISDLINENIDTAEGLKYLPTYLTEALSDYYYQKSPKKRREIIKAANTNGV
                     KNESALKMLGGMDQVVNVYNNFIPVFDKQFVSPISDNGDYYYNYRVIDTQLIANNRYF
                     HLVFTPRRKGGDTFEGDCWVHVGTFAIQKMNLRLGREANVNFVENLSLIQEYKMINDS
                     TWFLAKDKFVADVSPVGKDRPGFIGRKTTTYRNVIINDTSVLHELDKNKIMEEIITLP
                     NADEKKKEFWVEARHEPLSKTEAGIIKMIDTLTNAPVFQRFTKTMAFIGTGYKDIGNF
                     QIGPWMNWITSNGWEGFRMRFDLGTNKLFDKKIKLHGYLAYGFGDKNLKGMGEIFYLP
                     KKDPRMFLYASFTNDLDWGQNYYGEVSQDNIFALAVRKNGIPVKNIKVNEKRFEFYKE
                     TLPWMSNKLTITHKSYLPLRNLIPIDSFRADASGNPLTNFEISVRLRFAYLERFLESH
                     FYRTSLGSIYPIGEINISRGFSGVLKSSYSYTKVSASVSDYFKVPPYGNISWYIYAGK
                     TFGTLPYTMLDIAPGNELYYYNKYAFNMMNRWEFIHDRYAGINFEHNIGNGIFRLFPK
                     LRFRQFWTAKSLWGSLSAANKALNFKQGHTFQSLDGKTYLELGTGIDNILKVFRIDFV
                     WRVLPSTLKKDDDKTFGIFGSFRVAF"
     gene            8168..9667
                     /gene="glpK"
                     /locus_tag="PLO70_11815"
     CDS             8168..9667
                     /gene="glpK"
                     /locus_tag="PLO70_11815"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQZ75198.1"
                     /translation="MPSHYILALDQGTTSSRSLVFDEQGNIISQAQKEFRQLFPQPGW
                     VEHDAEEIWSTQYGTMAEAVAKANINMKQIAGIGITNQRETTVVWDKSSGQPIYNAIV
                     WQDRRTASDCDKLKSAGWAETIQQKTGLIIDAYFSATKLKWILDNVTGARQKAINGEL
                     AFGTIDSWLTWKLTNGEVHVTDVSNASRTMLYNIHSLQWDEELLKIFDIPASVLPEVK
                     PSSKIYGVTGSIIPDSRIPIAGIAGDQQAALFGQLCTQPGMVKNTYGTGCFMLMNTGE
                     KAIDSKNNLLTTIAWQIDNKTEYALEGSVFIAGAVVQWLRDELKIIRTSAEIEKLAAQ
                     VKDTEGVYIVPAFAGLGAPHWNQYARGSIFGLTRGSSNAHIARAALDSIAYQTFDVLK
                     AMEADAAIHIKELRVDGGATVNNQLMQFQSDILNTKVVRPKITETTALGAAYLAGLAV
                     GYWKSVEEIQEQWQVDKSFSPAMVDAKRNELVKGWQRAVNASIAWADAK"
     gene            9861..11342
                     /locus_tag="PLO70_11820"
     CDS             9861..11342
                     /locus_tag="PLO70_11820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HQZ75199.1"
                     /translation="MNRQHMIQQLESQPVWDICIIGGGATGLGTALDAASRGLKTILF
                     EQYDFAKGTSSKSTKLVHGGVRYLQQGNIRLVMEALKERGQLLKNAPHLVHNQQFIVP
                     NYKWWEKPFYGIGLKIYDWMAGKLGLGPSVLLSKEETLALVPTLDAEGLTGGVVYHDG
                     QFDDARLAISIAQTAVQNGAVVLNYIPVTGLLKMQQKVCGVWVKDSLTGKGWEVKSKV
                     VINATGVFTDTVMKMDDPKHESIISPSQGIHLVVDKEFLPGDTAIMVPRTDDGRVLFA
                     VPWHNKIVLGTTDTPVKRISTEPIPFEEEINFVLTHIGRYLTKDPQRSDVKSMFAGLR
                     PLVKGKSKKTAALARDHLVTIADSGMITITGGKWTTYRKMAEDVVNIAIEKAGLEKRN
                     CITADMKLAGHDKPVIPAEIKSLTTGELTTIIQKSIAEEMCMTVEDFLSRRTRQLLLD
                     AIVAMEKAPAVAAIMSKEMDKDQTWIIEQINNFNAIAKNYLPD"
     gene            11354..12064
                     /locus_tag="PLO70_11825"
     CDS             11354..12064
                     /locus_tag="PLO70_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HQZ75200.1"
                     /translation="MSPFLGEFIGTALLILMGGGVVANVVLNKTYGQNSGWIVITFGW
                     AMAVFLGVYISVKLGGSGHLNPAVTIALAATGAFKSSLVASYLLAQLAGAFTGAVLVW
                     LTYNKHFEASSDADLKKAIFCTAPAIRSPLHNLITEFIGTFVLMFGALAFTPATTSLG
                     TLDALPVTLLVLGIGLSLGGPTGYAINPARDLGPRIAHFLLPIPGKRDSDWSYSWIPI
                     VGPIAGALLAAWLFGLLN"
     gene            12104..12739
                     /locus_tag="PLO70_11830"
     CDS             12104..12739
                     /locus_tag="PLO70_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75201.1"
                     /translation="MKKVSATLILTFVSIFSFSQLRFYTEAGFHQSRPIFLGGNKNGT
                     PAETAFTGFTASVGSINKISNSFHFNTKISFIRKKFYESFFVGPDYYGSNEYKVSALQ
                     FHVLAEKNISLKRKLQFFPSLGFYTTMHTAGTVDYEIYNFGNYETGQRDIILNKNGDF
                     RRWDAGLTFGIKTQWQKYYFQFLTDAGIVEPSLYNSNKWGSVKFTVGYFFK"
     gene            complement(12805..>13253)
                     /locus_tag="PLO70_11835"
     CDS             complement(12805..>13253)
                     /locus_tag="PLO70_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ75202.1"
                     /translation="LLGKALGGGMPLGAFIADKKLMDAFTDNPVLGHITTFGGHPVCC
                     AAGMAGMKALLEEGWIATVKGKEELFTSLLDNPKIKAVRSFGLWMAVEFDSFETNKQI
                     IDKCIAAGVLTDWFLFASDCLRISPPLIISEEQIKKACEVIVKACD"
BASE COUNT         4249 a         2624 c         2624 g         3756 t
ORIGIN      
        1 agcctacggg gcaaggatat acgatgccca gattgggaga tggaatcata tcgacccatt
       61 aagtgaaaag atgagaaggc actcccctta taattatacc tttaataacc caataagatt
      121 tattgaccct gatggtatga agcctgatga tattattcgg gttaattcac aaggttatat
      181 tacaaaagta gaaaaggctg acggtcccca caaagtaatg ttgggtaacc aagaattgaa
      241 atttattgac cagaactttg accagaagca atttcaggca atgttaccag aaaaatctgt
      301 aagatatgat tttgactatt ctgagaattc taccagaata tttacgccct actctagcaa
      361 agagatgtca actaatttca atgatttgga aatcggagag ataagggata aatttcaatc
      421 taaggacaat gcaaatccgc aagcacctgg agccctcttg tcggtaggat tgtacttact
      481 taaactgggg cataatgatt ttgacttcgc tgatgatatg acttctttga caaaagatgg
      541 gggtaatgta aatattggtc caccgattca aagtaagttt gcgaaggacg gaactcaggg
      601 gtttgtaaaa tttgagcata caaatgattt atataatgtt tacgatgcag gcaattttat
      661 gacagggaag gcattttcta tgattgggct ttcggaaaaa agaattctgg atggggcaga
      721 tacaaatagt cgaattacat tcaatggacc tgatacaaaa gctgaccaaa aagcgttgtc
      781 aaatggggta aagtaccctt gggtaatatg gagtaaataa actatcatgc gaccaggttt
      841 tgtttcttta atatgtttct ttctctgtgt tgttaaatac tggctgttta attacttaga
      901 attatttagt ccgcctgtag accgaattat aagttggtat attatactac cgatttgtat
      961 aattggtgtg atttttagta ttttctccgt gtttaggata tttaggaagc aaacaactta
     1021 caagtatttt gatattctgc tgtcagtgcc ctttctcatt ttatttgtgt attttttctt
     1081 cattaaatag gtaatgaacc aatcgggtaa aaggccaatc atcacctgcc tgaaaacttt
     1141 cttcttctaa gcctttttaa aatacttcaa aacacagctt ccttatctat tttgtacaac
     1201 tgattttgta cattgcagta agctctttga accagatatt actatccgtt cgggttaata
     1261 atggcggggg tatcgtcgaa ggctttggcg tttggtaacc cccagaacaa attcaagttt
     1321 aataaaggtt ctgagttgca gaataaagag ttcagtgatg gtagcggatt ggaattatat
     1381 gctacaaact ttagaagcct tgacccacaa ttaggaaggt tctggcagac agaccccaaa
     1441 catgactgtt cacagagttt atactctgcg atgaataata atccaatttt gtacaatgac
     1501 ccgttagggg atacaataag aattagtttt aaaacaggtt tcttagggat tttcggaagg
     1561 aaagctaatc ttacttacga tgaaaaaaat caacgttgga ataataagga tggaagtgca
     1621 tatactggaa agactacaaa gttttccaac atggttcttg gtgatttaaa aacgaaccag
     1681 aaaaacgatt tgggtaatgc aatagttagc aatttggcta cagatatatt agaccacagc
     1741 attatgagag gcgaccctaa tagtaatacc atcaatgatc ccaatattta ttacagtggt
     1801 tctgtaagcg ataatcaaaa aatacttcaa gggggcaaat ctaattcttc gccaagctat
     1861 gttgttttag gccatgaaat ggctcataag ttttcccgaa acttaggaat tagaaatctt
     1921 ccttggtttg gaactccacc agaccagcga ggagttgacg aatataatgc catgtattat
     1981 gagaatgttt taaggcaagc aaatgggtta cctctacgca cagcttatca agaagctaat
     2041 ggaataacac aaggtgttat ccttaatgct gcaggaaatc tacaatcact accacctaat
     2101 cttgcatctc aaaacatgaa cctgccttta tatcaaacat tacgaacatt tcttagatta
     2161 ttttaattca acagttatga gactatcaat attagggtta ttaattttta tttcttgcaa
     2221 tgcaccaaag aaaattatac atataaagga tttaactctt acacttacaa agaaagaatg
     2281 cggtagttat gaagtgaagg ataatgaaga attgattggc tatgtggtta agccatttta
     2341 tattgaagta gagaagaaag agaatcacat tgttcagttt gagaaattaa taaaagctaa
     2401 ctactccgat tattatgaca gattttacag gacatattgg ttttcaaaaa acagtagctc
     2461 tgatacgtta attttagtag caatgctatc taccaaacaa ttaagttcaa tcccaaattg
     2521 gcaatgcgaa gaacaagaag cagacactta taatctgcta tacaagaata aaagcaataa
     2581 caaacatccc cggtttttta cttacaattg tacaaagggg cgttttaaaa ttttgggaga
     2641 ccccgactga aaagaaatga aataacagta aagccagaca ctataaaaag agggctttgt
     2701 agttaaagct attatataat taccattcta attttcttga gaaatacatt atgataccta
     2761 aagccacaaa caaaccaata cttcccatca gcaatgcgta atcctgtaat tgtattatgc
     2821 tgaaaatgta actgtaaacc actgccagta caaaactgat aaacaaagcc aatttggatg
     2881 atttaagaat gcttcccaca taccaaccta ccagtccaat agtagcagat ccggcgatga
     2941 ggtaggccag gttaaaccct gtatattcag aaattgataa taacaaggta tagaacagta
     3001 ctagtgccag accagccagg ccatactgta ccaggtgtaa tggtctttta taaatagttt
     3061 ctaccaaaaa gaaagctgca aaagtgagca taatacaaag tagggcatac tttacggacc
     3121 gctcagtctt gttataacta tcaaccggta tcagtaaatc agccccgaat tgagccgccg
     3181 agaaattata aaaagtattt ttccagactt gtggcatcgc cctgttcata tatttccatt
     3241 tagccgtaaa gccactatca gttacctgtc ttgaatccgg aagttttatt cctgtaaacc
     3301 ccgggtcggg ccatacggaa ctcatctcta ttttattctc cctggccagc gcagaaaaaa
     3361 gtaactgttc cgatccgtta agagaaaaat gaatactgaa agtgcttccg cttgcagcat
     3421 tatcctgtgt tattggtact gatgtaaaaa aagcatcctc gagcggggtc tgaccggttg
     3481 cctgtgttgt aaaaacaaat ctactgtcat tccattttac aaaaaggtct tcattgatac
     3541 ctttcaactg gtcctgtact ttgaatagca atactgcttc attccacaaa atttgatctg
     3601 ctgcaatctc caatttctgc cacggaatgt tattaaatct tccggtcagc ataatatccg
     3661 ttttataaac agccacttca tagatacccc gctttctttt ttcaggaaat acttttgatg
     3721 aaacatctag catatctggc atgaagtacg cattttgtct ttgccagctt tggcttcctt
     3781 tttcatcaac cagcagtaac ttgtatggta tcattaataa agggccggtt accgcttgtc
     3841 gtccagccca cttactactg atatcagcaa tagcttcttt ttgccggccc tctctttctt
     3901 ttactacttc cataataaag ttagtaggta tccaaagcag catcgccatc aggaagatga
     3961 taatacattt aagaaataat tttcctttac cccaaaaggg agttgcgctt ttgattgatt
     4021 caagtgtttc catcattctt agatttatga attaaactga aaaattgttt cgaacgaatt
     4081 gttataacaa cagcagctga taagtaagcc ggccagcaca tccataacat ttcccggcta
     4141 atttcttctc tggcaaaaac aaggagcaag caaataatat ttataactat tgacagaatg
     4201 actgccaata tccaaaggcc aattttttca acctgggaat aagcagacat aatgataatc
     4261 gaaagcacac cccatgccat taaaaatgcc ggtgaagaag caaccatcaa aaaaaatatt
     4321 agagcaatga actccggaaa aaaaaagtca aatgattgtc ctgctgcaaa caggtaaaaa
     4381 tcatatcctg caagccccac taccagaaca agcagggata aaatccatgt atagaaagta
     4441 tataaaaaat aattcatttt aatttccaat aatttaaaaa gcactttgta tttcaaagta
     4501 taagtgcaaa aaaaaatcta cttcattcct ttgatcatat tttccaatgc aatgatgtga
     4561 tctgtaaatg ccttttcccc ggacttggtt actgaatagg tggtattggt cttctttccg
     4621 ataaaacctt tatgcacctt aataaaacca ttctcttcca ggtttaccaa atgagtggcc
     4681 aggttaccat ctgttacttc cagcatttct ttcaggtcat taaaattcac ctcctcgttt
     4741 accatcaata cactcatcac accaagtcgg atgcggcttt caaacacttt acttaaacca
     4801 gttataggat ttttcatact tttttcttct gatcaaacct tccacttatt attttctttc
     4861 atatttcgac cacataatga ttccataaat gatatgcaat acgccaaaac caattgccca
     4921 gaaccataaa ccatagccaa taaaaaacat attgaccaac cccaatacta tctcacaata
     4981 acccaaataa cgaatatctg ataaagtgta cttacttgca tttacgagag caaggccata
     5041 aaacagcagg catgccggtg ctacaaatat ccatatatca tacctgagca ttcccgcaat
     5101 gaaagcagct ccggtaacca gcggtactat catattccag gctaatcgtt ttgacgtata
     5161 atcccataca ggcagtccct gctttctggc tttcttccat gtcaggtaaa aggaagaagc
     5221 aaatgccatt gcaaaaacaa ctgccgccaa ccctgccagc ctgatcttga gccggctaaa
     5281 atcatcacca gaaaaaaaac cctggctatt ataactaccg taataattat tcagaatagt
     5341 attggcaaca acagcacctg ccagggcaca aacacctgct gctattccgc ttaaaccact
     5401 taatgaaata aagcggctgg accggtccat tatacggcgg atatctttaa tgtcctgcaa
     5461 agaatcagtt tgttggctgg tttcattcat aaaaagcact ttgaaataca aagtaagtag
     5521 agcttttcta catttccaaa ttcttttttc tgaccgatcc ggcttactgt caatttaaca
     5581 ctccctgata ttgttagccg ttgtattttt acagcataat gctcccgttg aaacaatacc
     5641 ttatcctatt tctccttaat atgctggtgg ctgccttacc tgtgctgtcg caaactaaaa
     5701 ccatcagggg aactgtcaaa gattcccata gtgaagagcc tatccccttt gcatcagtaa
     5761 gtttcaaaaa tactaccctt ggaaaactga ctgactcctc cggctctttt actttttatt
     5821 ttaataaatg gccatcagat acccttgaaa tcacctgtgt gggctatcaa ccctttcggt
     5881 tggtacttgc tactagcaac gattctatct taacaacaat actcatggaa aggggcactt
     5941 ttaacgaagg cgcccagctg aaagtcaaag taaataaagg gttattagtc tggagaaaga
     6001 tcgttcaaaa caaaccccgg aatgaccgtt accggtttga taatttttca tacgaattat
     6061 ataataaact ggaactggac ctgaagaata tcaattttga aaaattcgga aaattcaaac
     6121 cgttcaaacc gatcagcgat cttatcaatg aaaatataga taccgctgaa ggattgaagt
     6181 atctgcccac ttatcttaca gaagccctct ccgattatta ttaccaaaag agcccaaaga
     6241 aaagaagaga aataataaaa gctgccaata ccaacggtgt aaaaaatgaa agtgcattga
     6301 aaatgctggg gggtatggac caggtagtga atgtatacaa caacttcatt ccggtttttg
     6361 acaaacaatt tgtaagcccg ataagtgaca atggtgatta ctattataat tacagggtga
     6421 ttgacacaca gcttatcgct aataatcgct atttccacct tgtatttaca cctcgccgga
     6481 aggggggaga tacgtttgaa ggggattgct gggtacatgt tggcacattt gccatacaaa
     6541 aaatgaacct gcggctgggc agagaagcca atgtgaattt tgtggaaaat ctcagcctga
     6601 tacaggaata taaaatgatc aatgattcca cctggttttt agcaaaagat aaatttgtgg
     6661 cggatgtatc cccggttggc aaagaccgcc ctggttttat tggtcgtaaa acaaccactt
     6721 atagaaatgt aattattaat gacacgtctg tactgcatga actggataaa aataaaataa
     6781 tggaagagat tatcactctt cctaatgcag atgaaaagaa aaaggaattt tgggttgaag
     6841 caagacatga gccacttagt aaaacagaag caggcatcat caagatgatt gacactctta
     6901 ctaatgcgcc ggtctttcag cgtttcacca aaacaatggc atttataggt accggttata
     6961 aagacatcgg taatttccag atcggcccct ggatgaactg gataacctcc aatggatggg
     7021 aaggtttccg gatgcggttt gatttaggca ccaataaact ttttgataaa aagatcaagc
     7081 tgcatggcta cctcgcatat ggctttggtg acaagaacct gaaaggcatg ggagaaattt
     7141 tctacctgcc aaaaaaagat ccgcggatgt tcctttatgc ttcttttacc aacgacctgg
     7201 actggggaca gaactattac ggggaagtgt cacaggataa tatttttgcc ctggctgtaa
     7261 gaaagaatgg catccccgta aaaaatatca aagtcaatga aaaacgtttt gagttttata
     7321 aagaaacact tccctggatg tcgaataaac tgacgatcac acataagtct tatttgccat
     7381 tgagaaatct tattccaatt gattcattca gggccgatgc aagtggtaat ccgcttacca
     7441 actttgaaat ttcggttcgg ttgcgttttg cctacctgga aagattcctg gaaagtcatt
     7501 tttaccgtac cagtctcggc agtatctatc ctattggtga aattaatatc agccgcggat
     7561 tttcaggtgt attgaaaagc agctatagct atacaaaagt ttctgccagc gtatccgatt
     7621 atttcaaagt cccgccctat gggaatattt cctggtatat ctatgccgga aaaacatttg
     7681 gtaccctgcc ctataccatg ctggatattg caccgggcaa tgagttatac tattataata
     7741 aatatgcctt taacatgatg aaccgctggg agtttattca tgatagatat gccggaatta
     7801 attttgagca taatataggt aatggtattt tccgattatt tccaaagctg cgcttccggc
     7861 agttctggac agctaaatcc ctctggggaa gtttatctgc agccaataag gcgctgaatt
     7921 ttaaacaggg gcatactttc caatcacttg atgggaaaac ttatttggaa ttaggtaccg
     7981 ggattgataa tattttaaaa gtcttccgta ttgattttgt atggagagta ctgccctcta
     8041 cactaaaaaa ggatgatgat aaaacctttg gtattttcgg aagtttccgg gtagcatttt
     8101 aattcattga ctcctcctgc tcgcaatcaa agttttactt aacttaccaa ttacaaattt
     8161 tgattaaatg ccttcacatt acatactggc ccttgaccag ggtacaacca gcagccgcag
     8221 ccttgtattt gacgaacaag gcaatatcat cagccaggca caaaaagaat tcaggcaact
     8281 atttcctcaa cccggctggg tagaacatga tgcagaagag atctggagca cccaatatgg
     8341 tacgatggca gaggcagtag ccaaagccaa tattaatatg aagcaaattg caggcattgg
     8401 catcaccaat cagcgggaaa ctaccgtagt ttgggacaaa agttcaggtc agcccattta
     8461 taatgcgata gtctggcagg acagaagaac tgcttcagac tgcgataaac tgaaatcagc
     8521 cggatgggca gaaactattc agcaaaaaac agggttgatc atcgatgcgt atttctctgc
     8581 caccaaactg aaatggatat tagataatgt aacaggcgca agacaaaaag caataaatgg
     8641 agaacttgca tttggaacta ttgattcatg gctcacctgg aaactcacca acggagaagt
     8701 ccatgtcacc gatgtttcca atgcatccag aacaatgctt tacaatattc attccctgca
     8761 atgggatgaa gagttgctga aaatatttga tatacctgct tctgtgttac cggaagtaaa
     8821 acccagcagc aaaatctatg gagtcaccgg gagtatcata cctgattcaa gaattccgat
     8881 tgcgggtatt gccggtgatc agcaggctgc tttgttcgga cagctatgta cacaacccgg
     8941 tatggtgaag aatacctatg gaaccggttg ctttatgctt atgaataccg gggagaaagc
     9001 tattgattcc aaaaacaatt tactgacaac tattgcctgg caaatagata ataaaacgga
     9061 atatgcattg gaaggaagtg tattcattgc cggcgcagtt gttcaatggc tccgggatga
     9121 attgaagatc atccggactt ctgccgaaat agaaaagtta gccgcacagg tgaaagatac
     9181 tgaaggtgtt tatatcgttc ctgcttttgc cggcctggga gcaccacatt ggaaccaata
     9241 tgccagaggc agcatttttg ggcttacaag aggaagcagc aatgctcata ttgcaagagc
     9301 tgctttggac agtattgctt accaaacctt tgatgtactg aaagcaatgg aggcagatgc
     9361 cgcgattcat atcaaagaat taagagtaga tggcggggcc accgtaaata accagctaat
     9421 gcaattccag agcgatattc tgaatacaaa agttgtgagg ccaaagatta ccgagaccac
     9481 tgcattaggc gcagcctacc tcgcaggcct tgctgttggt tattggaaaa gtgttgaaga
     9541 gatacaagaa caatggcagg tggataaatc tttctcgcca gcaatggttg atgcaaaaag
     9601 aaatgagctg gttaagggct ggcaaagggc agttaatgct tcgattgcat gggcagatgc
     9661 taaataacga aaagttgatg tcaccctgag tttatcgaag ggtgatcagg gaaaaagaaa
     9721 aaggcattat ttgacaggga ttggtatgag attaatgaat tagctaaatc gaataaaacg
     9781 tttcctgaat caggcttcga catccttcga taaactcagg atgacaaagc ctgacaaccg
     9841 ttattgtgca actgaaaaat atgaaccgcc agcatatgat acagcaactc gaatcccagc
     9901 ccgtatggga tatctgtatc attggaggag gcgcaacagg acttggaaca gcattagatg
     9961 cagcatcaag aggattaaaa acaattctct ttgaacaata cgattttgcc aaaggcactt
    10021 cttccaaaag caccaagctt gttcatggtg gtgtccgtta cctgcagcag ggaaatatca
    10081 ggttagtgat ggaagcattg aaagaaagag gccagttgct gaaaaatgct cctcacctgg
    10141 tccacaacca gcaattcatt gtacctaact ataagtggtg ggaaaaacct ttttatggaa
    10201 tcggacttaa gatctatgac tggatggccg gcaaactcgg tcttggccca tcagtattat
    10261 tatcaaaaga agaaacactg gcattggtcc ccacgttgga tgcagaagga cttaccgggg
    10321 gagttgtgta ccatgatggg cagtttgatg atgccaggct ggctatcagt attgcacaaa
    10381 cagctgtaca gaatggtgca gtggtgctga attatatccc ggttacaggt ttactaaaaa
    10441 tgcaacagaa agtttgtggt gtatgggtga aagattcact aactggtaaa ggatgggaag
    10501 taaaaagcaa agtagtgatc aatgctactg gtgtgtttac cgatactgtc atgaaaatgg
    10561 atgatcccaa acatgaaagt atcatcagcc ccagccaggg aatacattta gtggttgaca
    10621 aagaatttct acctggtgat acggccatca tggttcccag gacagatgat ggcagggtat
    10681 tgtttgcagt gccctggcat aataaaattg tattgggcac aaccgacact cccgtcaaaa
    10741 gaatctcaac agaaccaata cctttcgaag aagaaattaa tttcgtactt acacatatcg
    10801 gccgctacct gaccaaggac ccacaacgca gcgatgtaaa aagtatgttt gccgggttaa
    10861 ggccattggt aaaagggaag agtaaaaaaa cagcagcgct ggcaagagat catttagtaa
    10921 caattgcaga ttcgggaatg ataactatta ccggaggcaa gtggaccaca taccggaaaa
    10981 tggcggaaga tgtggtaaac atagccatag aaaaagccgg gctagaaaaa agaaattgta
    11041 tcactgcaga catgaaactg gcaggtcatg ataaacctgt aatacctgct gaaataaaat
    11101 ctttaactac cggtgagctt acaacgatca tccaaaaatc tatagctgaa gaaatgtgta
    11161 tgacggtgga ggattttctt tcccgcagaa caagacaatt attgctggat gcaattgttg
    11221 caatggagaa agcacctgcg gtggcagcaa tcatgagcaa agaaatggac aaagatcaaa
    11281 cctggataat agaacaaatc aataacttta atgcgatagc aaaaaactat cttcctgact
    11341 aaaacgattg cttatgtctc catttctcgg cgaatttatt ggcactgctc ttcttattct
    11401 aatgggtggt ggtgtggtgg ccaatgttgt attaaacaaa acttatggtc agaacagtgg
    11461 ctggatcgtg attacctttg gatgggctat ggcagtgttt ctcggtgtat atatttcggt
    11521 gaaactggga ggaagtggtc acctgaaccc cgccgttact attgccttag ctgcaaccgg
    11581 agcatttaaa tcttccttag ttgcttctta tttactggca caattagccg gggcttttac
    11641 cggagcagta ttagtgtggc tgacatataa caaacacttt gaagcttctt cagatgctga
    11701 tctgaaaaaa gcaatatttt gtacagcccc ggccatccgc agtcctttac ataatttgat
    11761 aacagagttc atcggaactt ttgttttaat gtttggtgca ctggccttta cacctgccac
    11821 aacttcatta ggaactttgg atgcattacc tgtaacctta ttagttttag gtattggctt
    11881 atccttaggc ggccctaccg gttatgcgat aaacccggca agagaccttg gaccaaggat
    11941 tgcgcatttt cttttaccta tacccggaaa aagagacagc gactggagtt atagttggat
    12001 accgattgtg ggaccgatag caggtgcttt gcttgccgcc tggttgtttg gtctattgaa
    12061 ttaatcatac aacatctttt tatttcccat tgtcactaac attatgaaaa aagtatcagc
    12121 aaccttaatt ctgacctttg tttccatttt ttctttttcc caattaaggt tttataccga
    12181 agcaggcttt caccagtcaa ggccaatatt tctgggtgga aacaaaaatg gaactccggc
    12241 agaaactgca tttacgggct ttactgcttc agttggttcg ataaataaaa taagtaactc
    12301 ttttcacttc aacactaaga ttagttttat ccggaaaaaa ttttatgaat ccttctttgt
    12361 cgggcctgac tattatggct cgaacgaata taaagtttct gcacttcaat ttcacgtcct
    12421 tgcggaaaaa aacatctcac taaaaagaaa attgcagttt ttcccttcac ttggttttta
    12481 taccactatg catacagccg gcacagttga ttatgaaatt tataattttg ggaattacga
    12541 aacgggacaa agagatataa tactgaataa aaatggcgat ttccggagat gggatgccgg
    12601 cctgactttt ggaataaaaa ctcaatggca aaagtactat tttcagtttc ttacagatgc
    12661 cggtattgtg gaaccctcct tgtacaatag caataaatgg ggatctgtca agtttacagt
    12721 cggatatttc tttaaataga ggtttccatc atagctctac gaagcgtccg gcctcacagt
    12781 tttatttttt gcctttggca aattttaatc acaagccttt acaattacct cacaggcttt
    12841 ctttatctgt tcttcggaaa tgatcagcgg cggagaaata cgcagacagt cagaagcaaa
    12901 caggaaccag tcagtcagca ccccggccgc aatacattta tcaatgatct gcttattggt
    12961 ttcaaatgaa tcaaactcca cggccatcca taagccaaaa gaacggacgg cttttatttt
    13021 tggattatct aacagtgagg taaatagttc ttctttacct tttactgtag caatccaccc
    13081 ttcttccagc aatgctttca taccggccat accggctgca cagcataccg ggtggccacc
    13141 aaacgtcgtg atatgcccca gtacgggatt atcggtaaaa gcatccatca gttttttatc
    13201 ggcaatgaat gctcccaatg gcataccgcc acccaatgct ttaccaagca aca
//
LOCUS       DAORTI010000096        13223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4629_length_13223_cov_6.653934, whole genome shotgun sequence.
ACCESSION   DAORTI010000096 DAORTI010000000
VERSION     DAORTI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_13223_cov_6.653934"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLO70_11840"
     CDS             <1..503
                     /locus_tag="PLO70_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75203.1"
                     /translation="EATDINALTDEYLRLAYHGLRAKDKSFNATLKTDFDKSISNIDI
                     VPQDIGRVILNLITNAFYVVNERQKAESGKLNAEGKMYEPTVEVSSKKINGTIEIKVA
                     DNGNGIPQKILDKIFQPFFTTKPTGQGTGLGLSLSYDIVKAHGGELKVETKEGEGSEF
                     VILLPV"
     gene            846..1307
                     /gene="tnpA"
                     /locus_tag="PLO70_11845"
     CDS             846..1307
                     /gene="tnpA"
                     /locus_tag="PLO70_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQZ75204.1"
                     /translation="MANTYHQIYLQTVFAVKYRQAVISKEWKQRVQAVIGNLINETGC
                     KTIIVNGVEDHMHCFVGLRPAVSVSELMKTVKAKSSKYINDHNLTKKRFGWQEGYGVF
                     SYRQRDVDMIFKYLQNQEEHHQKHSFRQEYLDLLKEFEIGYDEQYIFQELI"
     gene            1390..1482
                     /locus_tag="PLO70_11850"
     CDS             1390..1482
                     /locus_tag="PLO70_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ75205.1"
                     /translation="MSYDIVKAHGGELKVKTKEGEGTEFKIILP"
     gene            1506..5027
                     /locus_tag="PLO70_11855"
     CDS             1506..5027
                     /locus_tag="PLO70_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ75206.1"
                     /translation="MKKLVLVLLTLCTLGLQAQYKTPYFKMISVENGLPQTNVNFSFQ
                     DKLGYLWLGTANGLVRYDGYRLKEYPILNEEGMPVKPASINQIFEDSKGELWVIVYLQ
                     GVYQLDRKRDVFVRIPFGSKDDDLIKNKSPNSLKYDKNLSIFWGVANDTERDRSILYS
                     IDLVNKKLQVYSDLEKGNHFIPASRNIDISIDPAGKIWLLTDSLLSHFEPASKSFKPF
                     FTLPILSGVKRLISVRADPLDKDVVWLTTTSVIDVNQPPVNEIRGLLQLNIKTKKFTI
                     SLPGSKAPETLPVNCFHTFTDSLKRVWVSTEKGLALYDPKKGSFIQYPLNYPNKETHA
                     EYIAADKEGNLWITGNAGGLFFLDIKTGISSTYEPNGGPGDLPGNRNFGQVFIDRFNT
                     LWINIPNEGIAWRDVQKTQFSPVAINPFPKKNTVKEIPHQYDLVGTQGDSICYLTDST
                     SLLAWNTIKNSFTSIDLKNKKLYRSIINIAKSKDGNLWLACGANGVVQYDAVTRNITT
                     YQYDPKDSNTLSSPFVIDVAADPDGTIWIATANKGINSFNPRTKKFSQYPFAINNGAR
                     QVKDSLDDQTATCLYIDKDGVIWIGTNNGAVNSFDPRTKKFRSYLNYKEGFFAITSMY
                     EDSRKRFWVSTYLSGLYLLDKKTGALQRFSEKEGLIHNSVFSVKEDAAGNIWTASQKG
                     MSRINPATNAITNFGSAKPWMWEYFIKNLYDRNGLFNVPGRNGIIRFNPLQLDESKTP
                     PIVFIESVSYRSPGKSTDSLMFTEGRENIRLKYNENRLTFQFVALHFTNPEQNQYAYQ
                     LEGYDKEWMQAGTQRSVTYSNLSPGTYTFKVKASNSEGVWNETGAVFTITIRPPWWKT
                     IGAYIIYGLLFLALVYALDRFLRRRLVQKERERNQARELAQAKEIEKAYNELKATQQQ
                     LIQSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNSELIAEMKEELAKGNIEEAKTIA
                     NDIDENEQKIIFHGKRADGIVKGMLQHSRSSSGQKELTDINALADEYLRLAYHGLRAK
                     DKSFNATMKTDFDETIGNINIIPQDIGRVILNLITNAFYVVSEKAKAEGGKQNAEGKA
                     YEPSVSVSTKKINGIVEIKVKDNGNGIPPKVLDKIFQPFFTTKPAGQGTGLGLSLSYD
                     IVKAHGGEIKVTTTEMEGTEFTIVLPA"
     gene            5176..5709
                     /locus_tag="PLO70_11860"
     CDS             5176..5709
                     /locus_tag="PLO70_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQZ75207.1"
                     /translation="MKQKTSPFTLFFTVIAAVILLITACSTPEKKEPEKADPVAANIK
                     MYSQVWDEIMNKGKLDLFNDSNFTKDVVMHASPSDIVGIDSARAYYANFITGFSNIQF
                     TIKDIFGQGEKLVKHWNFKGTHSGNFFGIPATGKSVSLDGTTLVRMSNGKIAEERDFF
                     DNMDFMTQLGLIPAAGQ"
     gene            5789..6958
                     /locus_tag="PLO70_11865"
     CDS             5789..6958
                     /locus_tag="PLO70_11865"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQZ75208.1"
                     /translation="MKIILAPLFICCFLFATAQPYKKLHFDAILVDTHNDIPSTAIEK
                     GLSFDMDLGNKTNSDLRRMKAGGVDVQLFSIWCDGNQQNPFAWANREIDTVMAWTNRN
                     PGDMMQAFSPKEIHTAVKEKKLAVMFGVEGGHMIENDLNKLVALHNRGVKYMTLTWNN
                     STDWATSALDETTKGDSLKHKGLTDFGKQVVKKMNELGMLVDLSHTGEQTFRDVMNTT
                     TKPVLVSHSCVYSICPHRRNLKDEQIKAIGKNGGVIHINFYSGFIDSAFERRSEAFTQ
                     KHKAERDSLLKLNPEPYYMEVYLFEKYPDEVKALRPPLSAVIDHLDYIVKMIGVDHVG
                     LGSDFDGVNSLPQQLDDVTTYPLITKELVKRGYSKKEIRKILGGNFLRVLEANSK"
     gene            6999..8135
                     /locus_tag="PLO70_11870"
     CDS             6999..8135
                     /locus_tag="PLO70_11870"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HQZ75209.1"
                     /translation="MKAKSIKGKSTDEIKKAFEQCKADGFKPTLALVFLSAMNEIEAI
                     CSLLDKEGISIFGASTFAEFTEQEAENTGIAVLLLDINPAYFKIALIDNGEGSGYEIG
                     CHIGETGTKTFANPAFIISTANYKIPGALIIQGMIEKAGADATIVGGKAGELINLEGK
                     VFTNTQSIDNGIIALILDQDKITLKGIAASGWKPAGTEKKITQCTGPWIYTIDDQPAF
                     DIVKKFLGNDIVEDKNKEGIVKLNIIYPLQLYREGGSPYMLPVLFANTEDNSVMLAEA
                     VPEGSVFRFSMPPDFEVIDMVIKSSVEVKEKEMPDADALVIFSCIGRLGSLGPMASKE
                     VEGLAATWNSPMIGLYSLGEFGRVANGHPEFHGTTVSWVALKEK"
     gene            8174..8479
                     /locus_tag="PLO70_11875"
     CDS             8174..8479
                     /locus_tag="PLO70_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HQZ75210.1"
                     /translation="MLKATVLYGHPTDPEAFEKYYAATHTPLALKINGMVKMELTKFI
                     SAPDGGKAAYYRMAELYFAGPAEMQQAMSSPEGQATAADLANFATGGATMIIGAVEN"
     gene            8500..9639
                     /locus_tag="PLO70_11880"
     CDS             8500..9639
                     /locus_tag="PLO70_11880"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HQZ75211.1"
                     /translation="MRAKSIKGKSSEEIQSALQQSMTDGFKPTLAIAFISVKQDRKAI
                     CKLLDEKSIAIFGATTNGEFIDENHSKNSAAILLLDMNPDHFRVLFAEYPEKNYREVA
                     AGIAKTTKQLFANPAFLLSGSHMETDAEQLLFGFEEVVGKQVNVFGGMAGDDYAFKQQ
                     YVFTNNKESNRGMVVLAVDEDKIMIRGKATCGWKAVGTERIVTKSEGNHVYTVDNIPV
                     LDLTIKYGGLENIKEKDPEVAMEIAVNFPLQLQREFGDPVMRPGLLVDWNDHSFYCSG
                     SVPQGSKVRFSIPPDFDVMEKVITGVQALKAAEMPEADAVVVFSCAGRILALGPLMNQ
                     EIEGIKNVWNAPMAGMFSNAELARATGGNLEMHNCTTCCVALKEK"
     gene            9639..10799
                     /locus_tag="PLO70_11885"
     CDS             9639..10799
                     /locus_tag="PLO70_11885"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HQZ75212.1"
                     /translation="MKAKSIKGKSTEEIQSGLRESMADGFKPTIAIVFISVKQDREAI
                     SGLLEEKGIQIFGATTAGEFIDGEMEGGSIVMLLLDMNPAYFKLEFLETSQDTAFEDA
                     IKLGNTGKETFTNPAFIIANSGVTLDGEPFVEGIIQGFGKHPSSEDGEVTIFGGKAGD
                     DMALESTFVFTNGKSSDCALIALLIDESKIDVNGIATCGWQAIGTSKTVTKSEGSIVY
                     TIDDKPALDMLMNYLGVEIKPDGKREIVTFLSSWYYPLQVEREKTDPVIRTAMFANSE
                     ERSLICSGKVPQGSKIKFSMPPDFDSIDTVVQECRSIKDEAQQADALIMFSCVSRYLS
                     FGVVMKEEIEQVQKIWDAPMAGFFSYGEYGKSKTGKYDYHNNTCCLVTLKEK"
     gene            10801..11838
                     /locus_tag="PLO70_11890"
     CDS             10801..11838
                     /locus_tag="PLO70_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75213.1"
                     /translation="MNPHLQNILNFIQQDENLTAEQKNAITKSLKDADKELEITAFKL
                     DRTEKVKRTTAILLEETIEELEQKRKAIEETNSALQKSLEELKAAQAQLIQSEKMASL
                     GELTAGIAHEIQNPLNFVNNFSDVSTELVDEMNEEINKGNLEDAKQIAQDLKQNLEKI
                     NHHGKRAGDIVKGMLQHSRSSSGQKELTDINALADEYLRLAYHGLRAKDKTFNAAMKT
                     DFDETIGLIKIIPQDIGRVVLNLITNAFYAVTERKKLQPEGYEPVVAVGTKKEAGKVE
                     ICVSDNGTGIPQKVMDKIFQPFFTTKPTGQGTGLGLSLSYDIVKAHGGELKVETKEGE
                     GSEFIIQLPTN"
     gene            11843..>13223
                     /locus_tag="PLO70_11895"
     CDS             11843..>13223
                     /locus_tag="PLO70_11895"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75214.1"
                     /translation="MKIFVKIISLCLLPSLIAGQQENPYLSSLYRSLSDATSDTARMK
                     VCSNLGSYYLLEDRDSAGFYLEKALPIAVSLNLKLDEASILNKMGIILMQQEKFSKSL
                     EFYLKAIHIAKDPSIEKTIWDLSPGQSPMNARMLELSRSYDFIGLLNAYTGNWSENIK
                     NQLKNYREAERYANAAGNRGQIASITFHMGISYMNDGKLDSALLFIKKAQSEFSELKD
                     PQLGRSLIYLGETYERMGNFDLAAKTMLQALALLNQTNDYVHRGLGCISLSRVYVSLK
                     KIDSALYYAKESLKIFEKRKDPAWKRDAYNLLASCYDHLNKTDSATVYLKLAKSLSDS
                     LSVEERKNLLAFQDVVVDEQVKLEKLEKEKIQTKSKIRTYSMIAGLAILSLIGLILYR
                     NNKKEKKAKRVLEKTLKELKSTQTQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSE
                     VSSELLEEMK"
BASE COUNT         4468 a         2398 c         2925 g         3432 t
ORIGIN      
        1 aagaagctac agatattaat gccttaaccg atgaatattt gcggctggcc tatcatggct
       61 tgcgtgcaaa agacaaatcg ttcaacgcca ctttgaaaac tgattttgat aagtccatca
      121 gcaacatcga tatcgtaccg caggatattg gaagggtgat tttgaattta attaccaatg
      181 cattttatgt agtgaatgaa aggcagaagg ctgaaagcgg aaagcttaat gccgaaggaa
      241 aaatgtacga accaactgtt gaagtaagta gcaaaaaaat aaatggtacg attgaaataa
      301 aagtggcaga caacggcaac ggcatccctc aaaaaatact cgataaaata tttcaaccct
      361 tttttaccac caaaccaaca gggcaaggaa ctggtttggg attgagcttg agttatgata
      421 ttgtgaaagc acatggtgga gagttgaagg tagaaacaaa ggagggggaa ggaagtgagt
      481 ttgtgatatt gttaccggtg tgatatgagc cgtgccgatg gcactcgtta gcatcgtgac
      541 acttaggccg gactgaagtc cagccttaca agatagaacc gagcctacgg ctcttgagta
      601 tgagacgaag aagggtacta taataaacga gaggagtagt ttcattgtgc accggaatga
      661 attcggatgt tatagtatga agccggactt aaagccttgg actgtaagaa agatgaaaag
      721 ttatctttct tccggaaacc agtggcatag ccacggatat attttgtaac catggacttc
      781 agtccgtgat aagacaagta actatacaaa ataagagccg taggctcggt acataaaaaa
      841 aagcaatggc taatacctac catcaaatct acctgcaaac agtgtttgct gtaaaatacc
      901 gtcaggctgt tatcagcaaa gaatggaaac agcgagtaca agctgttatt ggaaacctta
      961 tcaacgaaac cggctgcaaa acaatcatcg taaacggcgt ggaagaccat atgcattgtt
     1021 ttgttgggct tagacctgct gtttcggtat ctgagttgat gaaaacagta aaagccaaat
     1081 catccaaata catcaatgac cacaacctga ccaaaaaaag gtttgggtgg caggaagggt
     1141 atggtgtatt ttcttatcgc cagcgggatg ttgatatgat ttttaaatat ctgcagaacc
     1201 aggaagagca tcaccaaaaa cattcattca ggcaggaata cttagattta ttgaaagagt
     1261 ttgaaattgg gtacgatgag caatacattt ttcaggagtt aatttaaact gaatttaaat
     1321 gatgcataat aaaatatttc aacccttctt taccaaccaa cgggacaagg aacaggcttg
     1381 gggttgagtt tgagttatga tattgtaaaa gcacatggcg gagagttgaa ggtaaaaacg
     1441 aaggagggag aaggcactga atttaaaata atattacctt aattggtata acctaaaaag
     1501 ttatcatgaa aaaattagtc cttgtattgc tgaccctttg tacacttggt ctgcaggcgc
     1561 agtataaaac accttacttc aaaatgatct ctgttgaaaa cgggttgcct cagactaatg
     1621 tcaatttcag tttccaggat aagctggggt atttatggct gggtacggcc aatggcctgg
     1681 tgaggtacga tggctaccgc ctgaaagaat acccgatttt aaatgaggag ggtatgccgg
     1741 tgaaaccggc ttccataaac cagatttttg aagacagcaa aggagaatta tgggtaatcg
     1801 tttacctcca gggtgtttac cagcttgacc gtaaacggga tgtatttgta cgtataccat
     1861 ttggcagtaa ggatgatgat ttaattaaaa ataaatctcc taatagcctg aagtatgata
     1921 aaaacctaag tattttctgg ggtgttgcca acgacaccga aagggacaga tctattctat
     1981 atagtatcga ccttgtcaac aaaaaattgc aggtatatag cgaccttgaa aagggtaatc
     2041 atttcatacc ggctagcaga aacattgata taagtataga ccctgcagga aaaatctggc
     2101 tgcttacaga ttcactgctt agccattttg aaccggcttc taaatcattc aagccctttt
     2161 ttaccttacc catactatcc ggcgttaaaa gattgatatc tgtccgggct gatccgttgg
     2221 ataaagatgt cgtttggtta actacgacca gtgttattga tgtgaaccag ccacctgtaa
     2281 atgaaattcg tgggctttta cagttaaata tcaaaacaaa gaaatttacc atttcactgc
     2341 ccggcagtaa agccccggaa actttgcccg taaattgttt tcacactttt accgattctt
     2401 taaaaagggt ttgggtttcc acagaaaaag gccttgcact atacgatcct aaaaaaggta
     2461 gttttattca atacccactc aattatccta ataaagaaac acatgctgaa tatattgctg
     2521 ccgataaaga gggaaactta tggataacag gtaatgcagg cggattattt ttcctggata
     2581 tcaaaaccgg aatttccagc acttatgaac ccaatggagg acctggtgat ctgcccggta
     2641 acaggaattt tggacaagtt tttattgacc gatttaatac cctttggata aatataccca
     2701 atgaagggat tgcctggcgg gatgtgcaaa aaacacaatt ctcccctgtt gccattaacc
     2761 cctttccaaa gaaaaataca gtaaaagaga tacctcacca gtatgacctg gtgggtactc
     2821 agggcgacag catttgttat ttaacagatt caaccagcct gctggcctgg aacactatta
     2881 aaaatagttt taccagtatt gacctgaaga ataaaaagct ttaccggtcg ataattaata
     2941 ttgcaaaaag taaagacggc aacctttggc tggcatgcgg ggcaaatggt gtggttcaat
     3001 atgatgccgt taccagaaat ataactacat accagtatga cccgaaagac agtaataccc
     3061 tgtccagtcc ctttgtaatt gatgtggcag cagacccgga tggtactatc tggatagcaa
     3121 cagcaaacaa agggattaat agttttaatc cacgaactaa aaaattttcc cagtatcctt
     3181 ttgccattaa caatggtgca agacaagtaa aggattcatt agatgaccag acagcaacct
     3241 gtttgtatat tgataaggat ggagtcattt ggattggcac caacaacggg gcggtaaaca
     3301 gttttgatcc ccgtaccaaa aagttcaggt cctacctgaa ttataaagaa ggcttttttg
     3361 ccattacctc aatgtatgaa gacagccgga aaagattttg ggtaagcacc tatctttcgg
     3421 gcttatacct gctggataaa aaaacaggtg cccttcaacg attttcagaa aaggaagggt
     3481 tgatacacaa ttctgtattt agtgtaaagg aagatgccgc agggaatatt tggacagcct
     3541 cccagaaggg gatgtcccgc atcaatcctg ctaccaatgc tattaccaac ttcgggtctg
     3601 ctaaaccctg gatgtgggaa tacttcataa aaaacctcta tgacaggaac gggttgttca
     3661 atgtgccggg cagaaatggc atcattcgtt tcaatccgct gcaactggat gaaagcaaaa
     3721 ctccgccgat agtatttatt gagtcagtga gttaccgttc tcccggcaag tcaactgaca
     3781 gtctaatgtt tacagaaggt cgggaaaata tccggctaaa atataatgag aacaggctca
     3841 catttcaatt tgtggcgctt cattttacca acccggagca aaaccagtat gcctatcaat
     3901 tagaaggcta tgacaaagaa tggatgcagg ccggaacaca acgttcagtt acatacagta
     3961 atctttcacc aggtacttat acttttaaag tgaaagcctc taacagtgaa ggtgtatgga
     4021 atgaaacagg tgcggtattt accatcacca tcaggccacc ctggtggaaa accatagggg
     4081 catatattat ttatggattg ttattcctgg cacttgttta cgctttagat cgtttcctgc
     4141 gtcgcagact ggttcaaaaa gagagggaaa gaaaccaggc cagggaattg gcacaggcca
     4201 aagaaattga gaaagcatat aatgaattga aggcaaccca gcaacagttg atccaatccg
     4261 agaaaatggc ttccttagga gaattgacag cgggaattgc ccatgaaatt caaaacccgt
     4321 tgaacttcgt caacaatttt tcggaagtaa actccgaact gattgctgaa atgaaggagg
     4381 aattagccaa aggaaatatt gaagaagcca aaacaatcgc caacgatatt gatgagaacg
     4441 aacagaaaat aatcttccat ggaaaaaggg ccgatggtat cgtaaaaggc atgctgcagc
     4501 acagccgttc aagttccgga caaaaagaac tcactgatat caatgcattg gcagatgaat
     4561 atttacgatt agcctatcat ggattaaggg caaaggataa aagcttcaat gccacgatga
     4621 aaacagattt tgatgaaaca atcggcaaca tcaatatcat tccgcaggat attggaagag
     4681 tgatacttaa tttaattacc aacgcatttt atgtggtgag tgaaaaagct aaagccgaag
     4741 gcggaaagca gaatgctgaa ggcaaggcat acgaaccatc cgtttcggtg agtacaaaaa
     4801 agataaacgg gatagttgaa ataaaagtga aggataacgg aaacggtatt cctccaaaag
     4861 tactggataa aatatttcaa cccttcttta ctaccaagcc ggccggccag ggaaccggat
     4921 tgggattgag tttgagttat gatattgtaa aagcacatgg cggtgaaata aaagtaacta
     4981 caacagaaat ggaaggaaca gaatttacaa ttgtattacc agcatagcta ttgcagagta
     5041 tcatcccgat agctaccagt atgaaggtga aaacaagggt aagcaagagt ttgaaattta
     5101 atattcaggt gcttttgcgc taatagtttt acattcaggt tctttctatt cttttgaaat
     5161 aaaaaacaat caacaatgaa acaaaaaaca agccctttta ccctcttctt tacagttatt
     5221 gctgcggtaa tcctgttaat tactgcttgt tcaacccctg aaaaaaaaga gcctgaaaaa
     5281 gccgacccgg ttgccgccaa tattaaaatg tactcgcagg tatgggatga gataatgaat
     5341 aaaggcaaac tggatctgtt caacgactcc aatttcacca aagacgtagt gatgcatgcc
     5401 agcccttccg atatagtggg tattgacagt gcccgggctt attatgccaa cttcattaca
     5461 ggtttctcca atatccagtt caccataaaa gatatttttg gacagggcga aaagctggtg
     5521 aaacactgga actttaaagg aacacattca ggaaattttt tcggcattcc ggcaacaggc
     5581 aaatcagtta gtttagacgg tacaacacta gtgcggatgt ccaacggaaa aattgcagag
     5641 gaaagggact ttttcgacaa tatggatttc atgacacagc tgggacttat ccctgctgca
     5701 gggcaataac aatttttcaa tcaatagcag tcctgttatc aaccagcgat aacaggactg
     5761 tgactttgtt catcacttaa tgatcattat gaaaataata ctggcaccat tattcatatg
     5821 ttgttttctt tttgcaactg cacaacccta caaaaaactg cattttgatg ctattttagt
     5881 agatacccat aatgacattc ccagcacagc gattgaaaaa ggtctgagtt ttgatatgga
     5941 cctcggcaac aaaaccaatt ctgacctgcg gcgtatgaaa gccggtggtg ttgacgtaca
     6001 attattttct atctggtgcg atggtaacca gcaaaaccct tttgcctggg ccaacaggga
     6061 aatagatacg gtaatggcct ggacaaacag gaatccgggg gatatgatgc aggctttttc
     6121 tcccaaagag attcatactg ctgtcaaaga aaaaaaactc gctgttatgt ttggggtgga
     6181 aggcgggcat atgatagaga atgacctgaa taaattagtg gcattacata acaggggtgt
     6241 taaatacatg acactgacct ggaacaattc cactgactgg gcaacctctg cgctggatga
     6301 aacaacaaaa ggcgattcat taaaacacaa ggggcttact gattttggca aacaggtggt
     6361 gaagaagatg aatgaactgg gtatgctggt agacctgagc catacgggtg aacaaacttt
     6421 ccgggatgta atgaacacca ctacaaaacc ggtactcgta tcacatagct gtgtatattc
     6481 tatttgtccg catagaagga acctgaaaga tgaacagata aaagcgatcg ggaaaaacgg
     6541 cggggtgatc catattaatt tttacagcgg gtttattgac agtgcctttg agagaagaag
     6601 cgaagcattt actcaaaaac acaaagcaga aagggattct ttgcttaaat taaatcctga
     6661 accctattac atggaggtgt atctatttga aaaatatccc gatgaggtaa aagctttacg
     6721 accacccctt tcagccgtaa ttgaccacct ggattatatt gtaaaaatga ttggggtgga
     6781 ccatgttggc ctgggcagcg attttgatgg cgtaaactct ttgccacagc aactggacga
     6841 tgtcacgact taccccctga tcacaaaaga actggtaaaa agaggatata gtaaaaaaga
     6901 gatacggaaa attttgggcg gaaatttttt gcgtgtactg gaagccaatt caaaataata
     6961 agtatatagt gtatgtatta aaatagccac atcaaattat gaaagcaaaa tcaataaaag
     7021 ggaaatcaac tgatgaaatt aaaaaagctt ttgagcaatg caaagctgat ggttttaaac
     7081 caaccctggc attagttttt ctttcagcaa tgaatgaaat agaggccatc tgttcattac
     7141 tggataaaga aggaatcagc atttttggag cttcaacgtt tgcagagttt accgaacagg
     7201 aagctgaaaa tacaggtatt gctgtgctgc ttctggatat caaccctgct tatttcaaaa
     7261 ttgctttaat agataatgga gaaggctcag gttatgaaat aggttgccat atcggagaaa
     7321 caggtacaaa gacatttgcc aatcctgctt ttattatttc tactgctaat tacaaaattc
     7381 cgggtgcatt gatcatccag ggaatgattg aaaaagccgg tgcagatgct actattgttg
     7441 gtggcaaagc cggggaattg ataaatttag aagggaaggt ttttaccaat actcaatcaa
     7501 ttgataatgg tatcattgca ttgatactgg accaggataa aataacttta aaaggaatcg
     7561 cggcctccgg ctggaaacca gcagggactg aaaaaaaaat cacgcaatgt acagggccct
     7621 ggatctatac catcgatgat caacccgcct ttgatattgt aaaaaaattc cttggaaatg
     7681 atattgtcga ggataaaaat aaagaaggca tcgtaaaatt aaacatcatt tatcctttac
     7741 aactttaccg ggaaggagga agtccctata tgcttcccgt cttatttgcc aatacagaag
     7801 ataattctgt catgctggct gaagcggtgc cggaaggttc ggtattccgt ttttccatgc
     7861 caccggattt tgaagtgatc gatatggtga taaaaagttc agtggaagta aaagaaaaag
     7921 aaatgccgga tgcagatgct ctcgttattt tttcctgtat tggccggtta ggatcgctgg
     7981 gacctatggc ttcaaaagaa gtagaaggac tggcggctac ctggaacagc ccaatgattg
     8041 gattatattc tcttggtgaa tttggccggg tggcaaatgg tcatccagag tttcatggta
     8101 ctactgtaag ctgggtagcc ctgaaagaaa aataaaacct gttacgaaaa tttataaacc
     8161 aaaactaaac tatatgctaa aagccacagt attgtacggt catccgactg accccgaagc
     8221 gtttgaaaaa tattatgcag caacacatac tccccttgca ttaaaaataa atggtatggt
     8281 caaaatggaa ctcacaaagt ttatttcagc cccggatgga ggaaaggctg cttattaccg
     8341 gatggcagaa ttgtattttg ccggccctgc cgaaatgcag caagcaatga gctcacccga
     8401 aggacaggcg actgctgccg accttgccaa ttttgctacc ggcggagcaa cgatgataat
     8461 cggtgcggtt gaaaactaat tttcataaac aaattctcaa tgagagcaaa atcaatcaaa
     8521 ggaaagtcgt cggaagaaat ccaatcagct ttgcaacaaa gcatgacaga tgggttcaaa
     8581 ccaacgttgg caatcgcttt tatctctgtt aaacaagaca ggaaagctat ctgtaaactg
     8641 ctggatgaaa aaagcattgc cattttcgga gccaccacca atggtgaatt cattgatgag
     8701 aatcattcta aaaattcagc agctattctt ctgttggata tgaaccctga tcatttccgt
     8761 gttttgtttg ctgaataccc cgagaaaaac taccgggaag ttgcggcagg gatcgctaaa
     8821 accacaaaac aattatttgc aaaccctgca ttcctgctct ctggaagtca tatggaaaca
     8881 gatgcagaac aattgctctt cggttttgaa gaggtggtag gaaagcaggt gaatgttttt
     8941 ggtggcatgg ccggtgatga ttatgcattc aaacaacaat atgtttttac caataataag
     9001 gaaagtaacc ggggtatggt agtattggca gtggatgaag ataaaattat gatcagggga
     9061 aaagctacct gcggctggaa agcagtaggc actgaaagaa ttgtgacgaa aagtgagggc
     9121 aaccatgtct acacagttga taatatccct gtactggact taacgataaa atatggtggc
     9181 cttgaaaaca taaaagagaa agaccccgaa gtagcgatgg agatagcagt aaattttcca
     9241 ttacaattac aacgggaatt cggtgacccg gtcatgaggc ccggtttgct ggtggattgg
     9301 aatgatcatt cattttattg cagcggttca gtaccacaag gttcaaaggt ccgcttttct
     9361 attcctcctg attttgatgt aatggaaaaa gtgataacag gtgtgcaagc tttgaaagct
     9421 gctgaaatgc ctgaagcaga tgcggtggtc gttttcagtt gtgcaggaag aattctggca
     9481 ttaggtccgt tgatgaacca ggaaattgaa ggaataaaaa atgtatggaa tgccccgatg
     9541 gccggcatgt tttccaatgc agagctggca agggctaccg gtggaaacct ggaaatgcat
     9601 aattgtacta cctgttgtgt ggcattaaaa gaaaaataat gaaagcaaaa tcaatcaaag
     9661 gaaaatcaac tgaagaaatt caatcagggt tgcgggaaag catggccgat ggattcaagc
     9721 caactatagc cattgtattc atatctgtta aacaggacag agaagctatt tccggattat
     9781 tggaggaaaa aggcatacag atatttggcg ctaccactgc aggcgaattt attgatggcg
     9841 aaatggaagg aggcagtatt gttatgctat tgctcgacat gaatccggct tattttaaat
     9901 tggaatttct tgaaacaagc caggataccg cctttgaaga cgcaatcaaa ctcggcaata
     9961 ccggaaaaga aacctttacc aaccctgcgt ttattatagc aaacagtggc gtaaccttag
    10021 atggtgaacc atttgttgaa ggtattatac aaggttttgg aaaacatcct tcgtcggaag
    10081 atggggaagt aaccatattt ggaggtaagg caggagatga tatggcactt gagtcaacat
    10141 ttgtttttac caacgggaaa agtagtgatt gtgcattaat agcactcctt attgatgaat
    10201 ctaaaataga tgttaatggt atagctactt gtggctggca agccattggc acttcaaaaa
    10261 cagttactaa aagcgaaggc agtattgtat ataccatcga tgataaaccg gcattggata
    10321 tgttgatgaa ttatcttggt gtagagataa agccggatgg caaaagggaa atcgtaacct
    10381 ttctaagctc ctggtattat ccattgcaag tggaacggga aaaaacagat cctgtaatac
    10441 gtacagctat gtttgccaat agcgaagaac gttcacttat ttgttccggc aaagtgccgc
    10501 aagggtcaaa aataaaattt tcgatgccgc ctgattttga ttcaatcgac acggttgttc
    10561 aagaatgcag gagcattaag gatgaagcgc agcaagccga tgctttgatc atgttttcat
    10621 gcgttagcag atatttatcg tttggggttg tgatgaaaga ggagatagag caggtgcaaa
    10681 aaatctggga tgcgcccatg gcaggctttt tctcctatgg cgaatatgga aaatcaaaaa
    10741 ccggtaaata tgattatcat aataatacat gctgcctggt aacattaaaa gaaaaataaa
    10801 atgaatccgc atttgcaaaa catattaaat tttattcagc aggatgaaaa cctgactgct
    10861 gaacaaaaaa atgcaattac aaaatcgttg aaagatgctg ataaagaatt agagataaca
    10921 gcttttaaat tagacagaac cgaaaaagta aaaagaacaa cggctatttt attagaagaa
    10981 accattgaag agctggaaca aaagagaaaa gccattgaag aaacaaattc ggctttgcaa
    11041 aaatcactgg aagaattaaa agcagcacag gcacaattga tccaatcaga aaaaatggct
    11101 tcactcggag aacttactgc aggcatagcc catgaaattc aaaacccgtt aaacttcgtt
    11161 aataattttt cggatgtaag tacggagctg gtagatgaaa tgaacgaaga aataaataaa
    11221 gggaacctgg aagatgcaaa acagatagca caggacctga aacagaatct cgaaaaaata
    11281 aatcatcatg gcaaacgggc cggcgatatc gtaaaaggaa tgttacaaca cagccgcagc
    11341 agcagcgggc agaaagaact gacagatatc aatgcccttg ccgatgagta tttacgatta
    11401 gcctatcatg gtttgcgggc caaagacaaa acctttaatg ccgccatgaa aaccgatttt
    11461 gatgaaacta tcgggttgat aaaaataata ccgcaggata tcggccgtgt ggtactcaat
    11521 cttatcacca atgcttttta tgcagttacg gaaagaaaaa aactgcaacc ggaaggctat
    11581 gaacctgtgg tagctgttgg cacaaaaaaa gaggcaggta aggtggagat atgcgtcagt
    11641 gacaacggaa ctggtatacc gcaaaaagta atggataaga tttttcaacc cttctttacc
    11701 accaaaccca ccgggcaggg aacaggtttg ggattgagtt tgagttatga tattgtaaaa
    11761 gcacatggtg gcgaattgaa agtggaaaca aaggaaggtg aaggaagtga gtttattatt
    11821 caattaccga ctaattaaaa aaatgaaaat ctttgtaaaa ataatttcat tatgcttatt
    11881 gccatcatta attgccgggc aacaggaaaa cccatatttg agtagtttat acagatctct
    11941 atccgatgcg acaagcgata ctgcccgaat gaaagtctgt agtaacctcg gatcctatta
    12001 tcttcttgaa gatcgtgata gtgcaggttt ttatcttgaa aaagccttgc ctattgctgt
    12061 cagcttaaat ctaaagttag acgaagcctc tatattgaac aaaatgggga ttatcctgat
    12121 gcaacaggaa aagttttcaa agtcattgga attctattta aaagcgatac atattgcaaa
    12181 agacccctct attgaaaaaa ccatctggga cttgtcgccg ggccaaagcc caatgaatgc
    12241 acgaatgctc gagctgagca gaagctacga ttttatagga ttgctaaatg cgtataccgg
    12301 aaactggagt gagaacataa aaaatcaatt gaaaaattat cgggaagccg aaagatatgc
    12361 caatgcagcc gggaacagag gacagattgc atctataact tttcacatgg gtatttctta
    12421 tatgaatgat gggaaattag attctgcact cctatttatc aaaaaggccc aatcagaatt
    12481 ttcagagttg aaagatccgc aattaggtcg atcattaata tacctaggtg aaacctatga
    12541 gaggatggga aattttgatt tggccgctaa aacaatgttg caggcattgg cgcttttaaa
    12601 tcagacaaat gattacgtac atagagggct tggatgtatt tcattaagtc gtgtatatgt
    12661 aagtttaaaa aaaattgact ccgctttgta ttatgcaaaa gaaagcttga aaatttttga
    12721 aaagcgtaaa gacccggcgt ggaaaagaga tgcctataac ctgcttgctt cctgttatga
    12781 tcacctgaac aaaaccgata gtgctaccgt gtatttaaaa ttggctaaat cattaagtga
    12841 tagtctaagt gtagaagagc gaaaaaatct tcttgcattt caggacgttg ttgtcgatga
    12901 gcaggtaaag ttggaaaaac tggagaaaga aaaaatacaa acaaagagta aaatcagaac
    12961 atatagtatg attgcaggtc ttgcaattct ttcgctgata ggtcttatat tgtaccgtaa
    13021 caacaaaaaa gaaaagaaag ccaaaagagt tttggaaaaa acgttgaagg aactaaaatc
    13081 tacgcaaact caactcatcc aatcagaaaa aatggcttca ctgggtgaac tcaccgccgg
    13141 tattgcacat gaaattcaaa acccgttgaa cttcgtcaac aatttttctg aagtaagcag
    13201 tgaattgctg gaagaaatga agc
//
LOCUS       DAORTI010000097        13148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4670_length_13148_cov_9.535095, whole genome shotgun sequence.
ACCESSION   DAORTI010000097 DAORTI010000000
VERSION     DAORTI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_13148_cov_9.535095"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLO70_11900"
     CDS             complement(<1..1190)
                     /locus_tag="PLO70_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQZ75215.1"
                     /translation="MIDYLSGLNDRQREAVLHKDGPIMIVAGAGSGKTKVLTTRVIHL
                     MAAHGVDAFNILALTFTNKAAKEMKERVEKILGNGEARNLYIGTFHSVFARILRFEAE
                     KIGYPRNFTIYDTDDAKSVVKTVINEMNLDDKHYKPNIVYNRISSAKNALVGPAEYRN
                     DYGLQQEDMRANRPAIGQIYDAYVKRCFKNGAMDFDDLLLKFYELLKNVPESLAKYQH
                     KFKYILIDEYQDTNPAQYEIIKLLGAMHENVCVVGDDAQSIYSFRGATIENILQFQKD
                     YDDVKIVKLEQNYRSTKSILHVANEVIKNNKGQIEKVLFTENGEGEKIKLVRTMTDND
                     EGKFVADSIQEQKLRNHYSNKDFAILYRTNAQSRAFEEALRRMGLAYTIYGGISFYQR
                     KEIK"
     gene            complement(1291..1827)
                     /locus_tag="PLO70_11905"
     CDS             complement(1291..1827)
                     /locus_tag="PLO70_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75216.1"
                     /translation="MRTDIFFTIIAGCLFCLSCNHQQSPQQTGNGGESKPPAIADSSL
                     NTSWRVKGCAEKATRSDTKFPPSGEYNDYPDLQPGDEPGIKANGDSIVYSRFDQHLCC
                     RQVKVTIDKSGNNITINEFWFGKGCKCRCSSTVQAVIRRLPKGEYQVYGVATGTDPVD
                     DKPTAGRDTVLRQLLTIN"
     gene            complement(1838..3310)
                     /locus_tag="PLO70_11910"
     CDS             complement(1838..3310)
                     /locus_tag="PLO70_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75217.1"
                     /translation="MKTYVVIFALLLFGYAMQAQQYIIRYDLAGENISYLKVKKPGDT
                     STAPVIHLSKTNRVNLQLVNAANSYRRELSYIEKTETAEPIIIPGFGNIPTSNLSGGG
                     FPAIDFKSLGIGDIFKKTSDNKDAGFGTETKQQKLLKELFTSNYNTFNSSFSKWERAL
                     QLTQHCRVLWKELAGLRYSLQLPAEEVKKTARVKTQSVFPAAGDNPNLILVSNAAESP
                     AAVAAELNKNFSALQKTYSYFNEYEVQSTVADGLLLEATKRTELVNSTPASKADSKND
                     DVVNRIADLYGQILNDSYSQLTPLNITSKTMMAEIRFTPLIDSVTASALNIKRTDTIT
                     RLIPIYKKEPLRFRNTFGFSFVSFAENRWHYYLNPDSIIARETADQFQPVVVTYLHFY
                     SPSDKGFRWGGTFGAGLPVGGDNAKLNIMLGLSTFFGKNDPVSLTAGIAGAQVKKLSG
                     WKLGDKVNFSELSAANYTSVYRIGYFLALTFNPGSLNMKD"
     gene            3444..3686
                     /locus_tag="PLO70_11915"
     CDS             3444..3686
                     /locus_tag="PLO70_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HQZ75218.1"
                     /translation="MVLLHSFFSGMESSLVILYILLLSVWSVALVAIANGRFYDNTTK
                     LCWFFIVMVLNIIGILLFIFWGRKEVDYRNRNVSDA"
     gene            3706..5031
                     /locus_tag="PLO70_11920"
     CDS             3706..5031
                     /locus_tag="PLO70_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75219.1"
                     /translation="MRPESEKTLLKKRSCLLSLVLSSIVLILFTSQPAKGQSALRQLE
                     VMAGRSIGSLPAPAAPSPGGSSYKSPFQKTGYQLEQEAREHYQKSLKEYNNRNWSDAI
                     RLLKKAIRKDQYNQTYKTKLREAEAAQEREKQQNRELQEKLRQEEEVRRKAEEEKQRI
                     EKARLDKERKEQEAIKENLKKAEQTILAFRKDIKTAQLQLKNYTKALNNNNSELEKWG
                     QQVDQSYNSVLDNSKEYLAGMFIKYNLLGGLKKQFHKDVYKRMGNLWKSSNPEIQKWL
                     AKQLAAADLKAERVEDIVDFVSQGGDLAELLTADKEEAGYNLDVLLFINGVLESSGVA
                     GYDKFMENALGNMPGDYFEQAKMIGETYADLSAICYSWFAIRKLTASNEDIAQKVNII
                     SAGMEQRMEEIECLKKCLQKNTTRCLENCTGKTKWSTPPPPLLFNYRNW"
     gene            5035..7329
                     /locus_tag="PLO70_11925"
     CDS             5035..7329
                     /locus_tag="PLO70_11925"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HQZ75220.1"
                     /translation="MKTILAGLLPIILCLLFAVQTIQAQSSSRDMEMKVEELQLNRSP
                     VKTQNDLYSYYLIPYKLINNKFGYINATTGDTAIAFLYDSAGYFNEGLSPVLLNGKWG
                     YINTYGKQIIPFRYDSTGEFSGGLCFVSNDTLMGIINKTGKEVIPIQFTELYYVDDNL
                     VRVMKNNKYGLYNTAGVQLSPPRFDDMTNFYEERAWVKIGDKWGFINKEGVLLISPKY
                     ELADRFTEEMAWVKTDYGLYGYVNKLGEEVIYPMYEDAQSYSEGLAAVRTLMEGGISS
                     YIYIDKKGDMIISNVGEGISWANPFQGGYAVVNKGRDKTGLIDKKGIEVIPCQYEEIG
                     ELSDSMIAVKENGKWGYYKIYYSNALNSTSSSMPISCRFDEAASFTDGVAMVSSGDAY
                     YINKKGEYIKPARETTNGEEEGQQFRKEQFTIDYINTRGERIIMGRPGILSSLFFNGM
                     TPVAMNKKWGFMNKQGVLAIPFRYDSVGSFNEGLATVRLNGKWGFIYQDADTLTSCRF
                     DYAYNFHKGLAIVKMNDKYGYLDKTGRMAIPCRYDMAYTFKDSLALVRMGDQFGFINK
                     SGEMVIPCQYDDAYDFNEGLAPVQKDGMWGYINKHRQIIIPFYYEEAAAFSEGLAAVM
                     KDGKYGYIDIRGNTIVPFRYAAAYSFNQGRAIVMDEEEGMLMIDSKGNEHFAAQFRSG
                     FYEDLALVVNEKSKLGFINRQGQTIIPYQYDDAMIFREGLAAVAIKNKWGFINKQGIV
                     VIPLQFDAALLFNEGLGIIANFNK"
     gene            7346..7735
                     /locus_tag="PLO70_11930"
     CDS             7346..7735
                     /locus_tag="PLO70_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75221.1"
                     /translation="MKNLSKQAAKRNFRQEVILYSFSFLLLIMFSCANVQKNTENLLG
                     ASFNYEKQEISIDVVTSGCTAKSDFNFTVSNNSITVIRNKKDECKAMPEVARFFYSFK
                     DAGINPDKTYTIINKFIANPNLARITN"
     gene            complement(7790..8488)
                     /locus_tag="PLO70_11935"
     CDS             complement(7790..8488)
                     /locus_tag="PLO70_11935"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HQZ75222.1"
                     /translation="MKKILLMCFFSINLSGYLLGQDKSNLTISTTGSSNLKIIYAGKK
                     YSLQDRSQTFQGQNPGTYSLVIYQWQTKQSGGEYIKVYDGSIRLTAKKHLEFTVLRFG
                     KTAWDESDIAGDTWNENYTNPRPVTETGDNYYNNQQGVTATQFANVKKALGNEYNEEE
                     RLNLVKVVFKNNLFSSEQIKELAKNFYNEEKRLRFTKYAYDFCWDKGNYFTLAEIFYN
                     SGYRKDFIEYIGNR"
     gene            complement(8557..10602)
                     /locus_tag="PLO70_11940"
     CDS             complement(8557..10602)
                     /locus_tag="PLO70_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQZ75223.1"
                     /translation="MKNFLFAFVLISLQSAAQINPANITIARDSFGVPHIFAPTDTEV
                     AYGIAWAHAEDDFETLQLVVLSGKAKLGTGLGKKGAEGDYVIKLLRIRQTVEEQWNTL
                     SPDFIALIRGYVAGLNAYAKAYPKEVKYKQAFPFDEKDYLTAVVFSISLFCGVDNALP
                     QILGEKVATIPGFNPQGSNAFAMNSVKTTTGESFLAINAHQPNEGPVAFYEAHLQSEQ
                     GWNMLGGLFPGGCLIFHGTNENIGWAHTVNYQDKIDIYQLEMNPANKNQYRFDGQWVN
                     LEVNKAKLKVKGIPITIGKKIYWSKYGATVKTKKGVFSLRVPATMDIKALEQWYRMNK
                     AQNFTAFYKALSMTSLPMFNILYADRYDTIFYISNGKMPRRNPDTTYKWKSTIPGNTS
                     ATLWTEFKTIEELPQYINPQSGYLFNTNHSPFLATDVKYNLDMRKFDRTDGYETYHNN
                     RSQRVTELINGDKVDYPTFKRIKFDQQLPKELKYIYGIDSMLNLNANEYPAIQEMVTV
                     FQAWDRKSTVTSKGAAIFLLTYYYLADRLKGVPARLITQKEAVETYQYVYDYMMKHFG
                     KTDLVLGDIQKLVRGNTISPAWGFPDVLAPAFSELYTNGMRKVTGGDAYICFVRYPKD
                     GGLPIIESVNTFGASMHPDSPHFKDQMELFQQQRTKKMTLDKKEVLKQAKKIYNPAK"
     gene            complement(10642..12192)
                     /locus_tag="PLO70_11945"
     CDS             complement(10642..12192)
                     /locus_tag="PLO70_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75224.1"
                     /translation="MPNRTTDILFQLIKSLEKAEKRHFKLYIKRSSGNEDLKIVRLFD
                     AVDKLNDYDEKVILKKLEGVTKPQLANLKTHLYKQIMASLRLLKSTDSMDLQLNEQFD
                     YAHILYKKGLFMQSLRIIDRAKEIARANQKFNFLPQLIALEKRIEGLHITRHIQYRAD
                     TLANEANEISRHIDMVARLSNLALKLFSWFVQHGHARNEEDEKDIRQFMKESLPADVW
                     KLKGFYERLYLYQSYTWYAFIRQDFLQYYRYTQKWVDLFTEQPLMIRVETGHYIKAIH
                     NLLNAHFDLRNHRQFEKTLKLLEKVAQTDRVKDHDNFRIQAFIYISTARINQHFMQGT
                     FKEGLSLIPGIEKELENYDLFLDSHRILVLNYKFAMLYFGSGDYNTCIDYLQKIINDK
                     TNLRYDLQCYARLVHLMAHYELGNDMLMESLTKSVYRFMAKMKNLTVVEEAMFKFLRQ
                     SITLSTRQLKPEFEKFLHTIKHLEKNRFETRSFAYLDIISWVEGKVYGKPMGTIIHEK
                     YMQSKRRK"
     gene            12679..>13148
                     /locus_tag="PLO70_11950"
     CDS             12679..>13148
                     /locus_tag="PLO70_11950"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQZ75225.1"
                     /translation="MAIRIFITGGTFDKEYNELDGELYFKDTHMSDLLEMGRSKVHVE
                     IRTLMMVDSLEMTDEDRELIVHQCNNCDEHQIVITHGTDTMSETAKVLAKKVKDKTII
                     LTGAMIPIKFGSSDGLFNLGSALAFAQTLPPGVYVAMNGRYFTADNVRKNKQTGV"
BASE COUNT         3828 a         2713 c         2667 g         3940 t
ORIGIN      
        1 tctttgatct cttttcgctg gtagaaactg ataccaccgt aaatagtata cgcaagcccc
       61 attcttcgca gcgcttcttc aaaggcacgg ctttgtgcat tggtacgata taaaatagca
      121 aaatccttat tgctgtaatg attacggagt ttttgttcct gtatactgtc ggcgacaaat
      181 tttccttcat cattatccgt catcgtccgt accagcttaa ttttttcacc ttccccgttc
      241 tccgtaaata atactttttc tatctggccc ttgttattct ttattacttc attggcaaca
      301 tgcaggatgc tttttgtgct acgatagttt tgttccagtt ttacaatctt cacatcatca
      361 taatccttct ggaattgcag aatattttca atcgtagcac cccggaatga ataaatactt
      421 tgtgcatcat ctcccaccac acatacattt tcatgcatgg ccccgagtaa tttgatgatc
      481 tcatactgcg ccgggtttgt atcctggtac tcgtcaatta aaatgtattt gaatttatgc
      541 tggtatttgg cgagactttc gggtacattt ttgagcagct cataaaattt cagcaataaa
      601 tcatcaaagt ccatcgcccc gtttttaaaa cagcgcttca cataggcatc atagatctgc
      661 ccgatagcgg gacgattggc ccgcatatct tcctgttgca aaccataatc attcctgtat
      721 tccgccgggc ccaccaaagc gtttttggca gaagatatcc tgttgtaaac aatattgggt
      781 ttataatgct tatcatccag gttcatttca ttgatgacgg tcttgaccac actcttggca
      841 tcatctgtat cataaatcgt gaagttgcgg ggatagccga tcttttcggc ttcaaagcgg
      901 agtattcttg caaatacaga gtgaaaagtg ccaatatata aattccgggc ttccccatta
      961 cccaggattt tttccaccct ttccttcatt tctttggctg ccttattggt aaaggtcagt
     1021 gccagtatat tgaaagcgtc cacaccatgg gctgccatca ggtgaatcac tcttgttgta
     1081 aggactttgg tcttcccgct accggccccg gccacaatca ttatcggccc gtctttgtgc
     1141 aacactgctt cccgctgacg gtcatttaat ccgcttaaat aatcaatcat tcttttggtc
     1201 acccatagtt ttgcgaaggg tgattaggtc cgcaaattac gttgattgcc cgagaggctg
     1261 aaactgtatg gccattgtta gcaagtattt ttaattgata gtgagcaact gtctcaacac
     1321 agtatccctg cctgcagtgg gtttatcatc aaccggatct gtaccggtgg caacaccata
     1381 cacctggtac tctccttttg gcagccggcg gatcactgcc tgtacggtcg agctgcacct
     1441 gcatttacac cctttcccaa accaaaactc atttattgta atattatttc cggatttatc
     1501 aatggttact tttacctgcc tgcaacaaag gtgttggtca aacctgctat aaacaattga
     1561 atcaccgttt gcttttatgc ccggctcatc gcccggctgt aaatcaggat aatcattata
     1621 ctctccggaa ggcggaaatt ttgtatcact tcttgttgcc ttttccgcac agccttttac
     1681 ccgccaggaa gtgttcaaag aactatctgc tatcgctggt ggcttacttt cacctccatt
     1741 acctgtttgc tgaggtgatt gctgatgatt acaggacaga cagaataaac atccggctat
     1801 tattgtaaaa aaaatatctg ttctcattta ttttgattta atccttcata ttaaggctgc
     1861 cggggttaaa agtcagtgca agaaaataac caatcctgta tactgaagta taattagcag
     1921 cactcagttc agaaaaattt actttatccc caagtttcca tcccgacaat ttcttaacct
     1981 gtgcacctgc aattcctgct gtgagactta caggatcatt tttcccgaaa aatgtactta
     2041 accccaacat aatattcaac tttgcattgt caccgccaac aggaaggcca gcaccaaaag
     2101 ttccgcccca acgaaacccc ttatcgctag gtgaataaaa atgaaggtag gtgaccacca
     2161 ccggctgaaa ctggtcggct gtttccctgg caataatact gtcggggttt aaataataat
     2221 gccagcggtt ctctgcaaaa gaaacaaaac tgaatccaaa tgtattccgg aaccgaaggg
     2281 gctctttttt ataaattgga attaacctgg tgatcgtatc tgttcttttg atgttcaatg
     2341 ctgaagcagt cacagaatca attaacggcg taaacctgat ttcagccatc attgttttag
     2401 atgttatatt caggggagtt aactgcgaat agctgtcatt caaaatttgc ccatataaat
     2461 cagcaatacg gtttacaaca tcgtcatttt tagaatctgc cttcgatgcc ggagtgctat
     2521 tcaccagttc agttcttttg gtggcttcta aaagcagtcc gtcagcaacg gttgattgta
     2581 cttcatactc gttgaagtag gagtatgttt tctgtaaagc cgaaaaattt ttatttagtt
     2641 ccgcagctac tgccgcaggt gattcagctg cattgctaac caggatcagg ttgggattat
     2701 ctccggcagc cggaaaaacg gactgggttt ttacacgggc agtcttttta acttcttctg
     2761 ccggtaattg taaactgtat cgtaaacccg ccaactcttt ccataaaacc ctgcaatgct
     2821 gtgtcagttg caatgctctt tcccattttg aaaaagaaga attaaatgtg ttataattac
     2881 tggtgaataa ttctttaagc agtttttgtt gcttggtttc tgtaccaaac cctgcatctt
     2941 tattatcact ggtcttttta aatatatccc caataccaag acttttgaaa tcaatagcgg
     3001 ggaatccacc gccggaaagg ttactggttg ggatattacc aaaaccagga ataattattg
     3061 gttcagctgt ttcagtcttc tcaatataac taagctccct ccggtaactg ttggctgcat
     3121 taaccaattg caggtttaca cggtttgttt ttgacaaatg aataacgggt gccgttgatg
     3181 tatctccggg ttttttgact tttaagtaac tgatattttc acccgcaagg tcatagcgga
     3241 taatatattg ttgtgcctgc atggcatagc cgaataacag caacgcaaaa attaccacat
     3301 atgttttcat aaccgggttt taggaatcaa caagttagcg ggttgccaaa gaaggggtaa
     3361 tgacttatat cattcatatc cttgcttttc ctcattaaaa aaaaatgatt cccggtataa
     3421 ctttcgtaaa atataagact ggtatggttt tgctgcactc attcttcagc gggatggaaa
     3481 gtagccttgt aatactatat atactcctcc ttagcgtctg gtctgtagca ctggtagcca
     3541 tcgctaacgg acgcttttat gataatacca ccaaattgtg ctggttcttc attgtaatgg
     3601 tactcaatat cattggtatc ctgttattta tattctgggg aagaaaggaa gtagattata
     3661 ggaatagaaa tgtaagtgat gcctgaacgt attcaaacta accgtatgag acccgaatct
     3721 gaaaaaacat tactaaaaaa aagaagctgt cttttgtcct tggttttatc ttccatagtg
     3781 ctcattttat ttacgtcaca acctgcaaag gggcagtctg cgcttaggca actggaagtt
     3841 atggccggca gaagtattgg tagtttacct gctccagctg ctccatcccc tggcggtagc
     3901 agttataaaa gcccatttca aaaaaccggg tatcaactgg agcaggaagc aagagagcat
     3961 tatcaaaaga gtttgaagga atataataac aggaactgga gtgatgccat ccggctgcta
     4021 aaaaaagcaa ttcgtaaaga ccaatacaac caaacttata aaactaaact tcgggaggct
     4081 gaagctgcac aggaaagaga aaagcaacaa aacagggaac tgcaggaaaa actaagacag
     4141 gaagaagagg taagaagaaa agccgaagaa gaaaagcaac gtattgaaaa agccaggtta
     4201 gataaagaaa gaaaagaaca ggaagcgatc aaagaaaatt taaaaaaagc ggagcaaact
     4261 atccttgctt tccgaaaaga catcaaaaca gcacagttac agctgaagaa ttataccaaa
     4321 gcattgaata ataataattc tgaattggaa aaatggggtc agcaagtaga tcaatcttat
     4381 aacagtgtac tcgacaacag caaagaatac ctggccggca tgtttatcaa atacaatctc
     4441 ctgggtggtt tgaagaaaca atttcataaa gatgtatata agcgaatggg aaatctctgg
     4501 aaatcttcta acccggaaat ccaaaagtgg ctggccaaac aattggcagc tgcggacctc
     4561 aaagctgaaa gagtagaaga tattgttgat tttgtatcac agggtggtga cctggcagaa
     4621 ctcttaactg cagacaaaga agaggccggc tacaacctgg atgtactatt atttattaac
     4681 ggagtgctgg aaagttctgg tgtagccggt tatgataagt ttatggaaaa tgctcttggt
     4741 aatatgcctg gtgattattt tgaacaagcc aaaatgattg gcgaaaccta tgccgacctt
     4801 tctgctattt gttatagttg gtttgccatc cgtaaactga ctgccagcaa tgaggatata
     4861 gcgcaaaaag ttaatattat ttctgccgga atggaacaac gcatggaaga gatagaatgc
     4921 ctgaaaaaat gtttgcaaaa aaatacgacc cgctgcctgg aaaactgtac gggaaaaact
     4981 aaatggtcca caccaccgcc acctctactt tttaattacc gaaactggta actcatgaaa
     5041 acaattctcg caggattact gcctattata ctttgcctct tgtttgcagt tcaaacgata
     5101 caagcacaat cttcatcaag agatatggaa atgaaggttg aagaactcca acttaaccgt
     5161 tcaccggtta aaacccaaaa tgacttatat agttactacc tcatcccgta taaattaata
     5221 aataacaagt ttggatatat taatgccaca accggtgaca ctgccattgc atttctgtat
     5281 gacagtgcag ggtatttcaa cgagggtttg tcaccagtgc tactcaacgg caaatggggt
     5341 tatattaata catatggaaa gcagatcata cccttccgat atgactcaac cggtgagttt
     5401 tcaggtggac tttgctttgt aagcaatgac accctgatgg gcattatcaa taaaacaggg
     5461 aaagaggtca ttcctattca gtttacagag ctatattatg ttgatgataa cctggtcagg
     5521 gtaatgaaga ataacaagta tggtttatac aatactgcag gagtccaact gagtccgccc
     5581 aggtttgatg atatgactaa tttttatgaa gaaagggcct gggttaaaat aggtgacaaa
     5641 tggggtttta tcaacaagga aggtgtctta ctcatcagcc cgaagtatga actggctgat
     5701 cgtttcacag aagaaatggc atgggtaaaa acagattatg ggttgtatgg atatgtaaat
     5761 aagctgggag aagaagtaat atatccgatg tacgaggatg cacaaagcta cagtgagggt
     5821 ttagcggcag ttagaaccct tatggaagga ggtattagtt catacatcta tatcgataag
     5881 aaaggagata tgattatcag taatgtgggc gaaggaattt cgtgggctaa tccttttcag
     5941 ggcggctatg ccgtagtgaa caaaggccgt gataaaactg gcctgattga taaaaaaggc
     6001 attgaggtga tcccctgcca gtatgaagaa attggtgaac taagtgacag tatgattgct
     6061 gtaaaagaaa acggtaaatg gggttactat aaaatatatt acagtaacgc cttgaatagt
     6121 acttcgtctt ctatgcctat ctcttgccgt tttgacgagg ctgcttcttt tacagatggg
     6181 gtagcaatgg tcagcagcgg agatgcatat tatattaata aaaagggaga atatataaaa
     6241 cctgccaggg aaacaactaa cggagaggaa gaaggtcaac agttcagaaa agaacaattc
     6301 actattgatt atatcaacac caggggagaa agaataatta tgggcagacc cggtatatta
     6361 tccagcctgt tctttaatgg tatgacaccg gtagcaatga ataaaaaatg gggattcatg
     6421 aataaacagg gagtgttggc tattccgttc cggtatgata gtgtgggttc cttcaacgaa
     6481 ggccttgcaa ctgtaagact gaatgggaaa tggggtttta tttaccagga tgccgataca
     6541 ctcacctcct gccggtttga ctatgcttat aattttcaca aaggactggc catagtaaaa
     6601 atgaatgaca agtacggata cctggacaag accggcagaa tggcaattcc ctgccgctat
     6661 gatatggcat atacttttaa agacagcctt gcactggtgc gtatgggaga ccagtttggt
     6721 ttcataaaca aatcaggtga gatggtgatt ccctgccagt atgatgatgc gtacgatttc
     6781 aatgagggat tggcgcctgt acaaaaagac gggatgtggg gctatatcaa caaacataga
     6841 caaatcatca ttccctttta ctacgaagag gctgctgcat ttagcgaagg gctggccgcg
     6901 gttatgaaag atggaaaata cggctatata gacattagag gaaatacaat tgtacccttc
     6961 cggtatgctg ctgcctattc attcaaccag ggaagagcca ttgtgatgga tgaagaggaa
     7021 ggaatgctga tgattgatag caagggaaat gagcatttcg ctgcacaatt tcgaagcggc
     7081 ttttacgaag acctggcact ggttgtcaat gaaaaatcca aactggggtt tataaaccgg
     7141 cagggccaaa caataattcc ttaccaatat gacgatgcaa tgatcttccg ggaaggatta
     7201 gcagccgtgg ccattaaaaa caaatgggga ttcatcaata aacagggaat cgtggttatc
     7261 cccctgcagt ttgatgccgc tttacttttc aacgaagggc ttggtatcat agctaatttc
     7321 aataagtaat actaaaacaa agcacatgaa aaatttaagt aaacaagcag caaaaagaaa
     7381 tttcaggcag gaagtaatac tatatagttt ttcttttttg ttactgatta tgtttagttg
     7441 tgcaaacgtt cagaagaaca cagaaaattt actgggcgca tcatttaact atgaaaaaca
     7501 agaaattagt attgatgttg taacgagcgg ctgcacagca aaatcagatt tcaattttac
     7561 agtgagcaac aactctatca cggttattcg caacaaaaaa gatgaatgca aggcgatgcc
     7621 ggaagtcgcc cggtttttct attcctttaa agatgccggc atcaatcctg acaaaaccta
     7681 taccatcata aataagttta ttgccaaccc taacctggcc agaattacta actgaatgaa
     7741 atacaaaagg cgtgaaataa aattttacta tgtaaacgtt tgcagaatat tatctgttgc
     7801 caatgtattc aataaaatct tttctatatc ctgagttgta aaatatttct gcaagagtga
     7861 aatagtttcc tttatcccag caaaaatcat aagcatattt tgtgaaacgg agccttttct
     7921 cctcattgta aaaatttttg gctaactcct taatctgttc ggaggaaaac aagttattct
     7981 taaaaactac tttgaccagg ttcagccgct cttcttcatt atactcattg cccaatgctt
     8041 ttttcacatt ggcaaattgc gttgctgtta ctccttgctg gttattataa taattatccc
     8101 cggtctcagt gactggcctg gggttagtat aattttcatt ccatgtatcg ccggcaatat
     8161 cactttcatc ccaggctgtt tttccaaatc gtaatacagt aaactcaagg tgtttcttag
     8221 cggtaagtct tatagaaccg tcatatacct ttatatactc tccacctgac tgtttggttt
     8281 gccattgata aattaccagg ctgtaagtac ccgggttttg gccctggaaa gtctggcttc
     8341 tgtcctgcag gctgtatttt ttacctgcat aaataatttt caggttgctg cttcctgttg
     8401 tactgatcgt caggttactt ttatcctgtc caagtaaata acctgaaaga ttaatactaa
     8461 aaaaacacat tagtaatatc ttcttcatac tcgatttatt attcactaaa tatactactc
     8521 tgcttagcgt aaccttatac ccaacagtac gtagatctat ttggccggat tatatatttt
     8581 tttagcctgt ttcagtactt ctttcttatc caaagtcatc ttcttggttc gttgctgctg
     8641 gaatagttcc atttggtctt taaaatgcgg agaatccgga tgcatagagg cgccaaaagt
     8701 atttacggat tcaatgatgg gcaatccccc atctttcggg taacggacaa aacagatata
     8761 cgcatcaccg cccgtaactt ttctcatccc gtttgtatac agctcagaaa aagccggggc
     8821 cagtacatcc ggaaaccccc aggccgggct aatagtattg ccccttacca atttttgaat
     8881 atcacccaat acgagatccg ttttaccaaa atgcttcatc atataatcat atacatactg
     8941 ataggtttct actgcttctt tttgggtgat cagcctggca ggtactcctt tcaatctatc
     9001 tgccaggtaa taataggtca gtaaaaaaat agcggctcct ttgctggtaa ctgttgattt
     9061 tctgtcccag gcctgaaaaa cagtaaccat ttcctgtatt gccgggtatt cattggcatt
     9121 taagttcagc atgctgtcaa tgccataaat gtatttgagt tcctttggta actgctggtc
     9181 aaattttatt cgtttgaacg tgggataatc aactttatcc ccgttaatca attctgttac
     9241 ccgctgactg cgattgttat gataagtttc atacccatca gtccggtcaa actttctcat
     9301 atccaggttg tacttaacat ctgtagctaa aaaaggagag tggttggtat taaacaaata
     9361 gccggattgc ggatttatat attgtggcaa ctcttcaatt gttttaaact cagtccacaa
     9421 agtggctgat gtattcccgg gtatagtact tttccattta taggttgtat caggatttcg
     9481 tctgggcatt ttcccattac tgatataaaa tatcgtatcg tacctgtctg cataaagtat
     9541 attgaacatg ggcaggctgg tcatgctaag tgccttgtag aacgctgtaa agttttgtgc
     9601 tttgttcatc ctgtaccatt gttccagcgc tttaatatcc attgtggcgg gaacccgcag
     9661 cgagaaaacg cctttttttg ttttcaccgt tgccccgtat ttgctccagt atattttttt
     9721 accgatggtt ataggaatcc cttttacttt cagtttcgct ttattcactt ccaggtttac
     9781 ccattgaccg tcaaatctgt attgattttt attagccgga ttcatttcca gctggtagat
     9841 atctatttta tcctggtagt tgaccgtatg agcccagcct atattttcat tcgttccatg
     9901 aaaaataaga cagcctccgg gaaataaacc gcccagcata ttccatccct gctcgctttg
     9961 caggtgagct tcataaaatg ccactggtcc ttcattaggc tggtgtgcat tgatggccaa
    10021 aaaagattct ccggttgttg tctttaccga gttcatcgca aatgcattgg agccctgcgg
    10081 attgaacccc ggaatagttg ctaccttttc cccgagtatc tgtggtagtg cattatccac
    10141 gccgcagaaa agactgatag aaaaaacaac agctgttaaa taatccttct catcaaaagg
    10201 aaaagcctgt ttgtatttta cctccttagg atatgctttt gcataagcat tgagaccggc
    10261 aacatatccc ctgatcaaag caataaaatc aggactgagc gtattccatt gttcttccac
    10321 tgtttggcgt attcgtaaca gcttaatgac atagtcaccc tctgcacctt ttttacccag
    10381 gcctgtacct aatttagctt tgccgctcag taccaccagc tgtaatgttt caaaatcatc
    10441 ttcggcatga gcccaggcaa tgccatatgc cacttcagta tcggttggag caaaaatatg
    10501 cggcaccccg aaactgtcac gggcaatagt aatatttgcg ggattgattt gagctgcgga
    10561 ttgtaaactg attaaaacaa atgcgaatag aaaatttttc atgcctgaaa ataaacaaaa
    10621 agatcttgca ccggaagttt cctatttcct tctcttgctc tgcatatatt tttcatgaat
    10681 gatcgttccc attggcttgc catatacttt accctccacc caggagatga tatcgagata
    10741 ggcgaaggaa cgggtttcaa agcggttttt ctcaaggtgc ttaatggtat gcagaaactt
    10801 ttcaaactcc ggttttaact ggcgggtaga taaagtaatg gattgacgca gaaatttaaa
    10861 cattgcttcc tccaccactg taaggttttt catttttgcc atgaatcgat aaacagattt
    10921 cgtcagcgac tccatcagca tatcatttcc caattcgtaa tgggccatca ggtgtaccag
    10981 ccgggcatag cattgcagat cgtatcgcag gttggtctta tcattgatga tcttttgcag
    11041 atagtcgata caggtattat aatcaccact gccaaaatac aacatggcaa atttgtaatt
    11101 cagtaccagt atccggtggc tatcgagaaa gaggtcatag ttctccagtt ccttttctat
    11161 acccggtatc agggagagcc cttctttaaa cgtaccctgc ataaaatgct ggttgatgcg
    11221 ggccgtactg atatagataa atgcctgtat ccggaagttg tcatgatctt ttacccggtc
    11281 tgtttgtgct actttctcca gtagttttaa tgttttctca aactgacggt gattgcgcaa
    11341 atcaaaatgg gcattcagta agttatgaat ggcctttata tagtggcctg tttccactct
    11401 aatcattaaa ggctgttccg taaaaaggtc aacccatttt tgagtgtagc gatagtattg
    11461 taaaaagtcc tgccggatga acgcatacca ggtgtaactc tggtacaggt agagtctttc
    11521 ataaaaacct ttcagtttcc acacatcagc aggcaggctt tctttcatga attgccggat
    11581 gtctttttca tcctcctcat tcctggcatg gccgtgctgc acaaaccaac taaaaagttt
    11641 cagtgccagg ttggaaaggc gggctaccat atcaatatgc cggcttattt cattggcctc
    11701 atttgccagc gtatccgccc ggtattgtat atgcctggtt atatgcagtc cctcaatcct
    11761 tttttccaaa gcaatcagtt gtggcaggaa attgaatttc tggttagccc ttgctatttc
    11821 tttcgcccgg tctattatcc ggagactttg cataaacaaa ccttttttat aaaggatatg
    11881 tgcatagtcg aattgctcat tcagctgcag atccatactg tcggtgcttt tcagtaaccg
    11941 gagactggcc atgatctgct tataaagatg ggtcttcagg tttgccagct ggggtttggt
    12001 cactccttcc agctttttca gtattacttt ttcatcgtaa tcattcagct tatctaccgc
    12061 atcaaaaagc cggacgattt tgaggtcttc attaccggaa ctgcgtttga tatagagctt
    12121 aaaatgcctt ttttctgcct tttccagcga ttttatcagt tgaaataata tgtcggttgt
    12181 gcggttgggc atcatagaga gtttttgctg aaaccttctg ccagcaagga ttttgctcaa
    12241 caccccctcc tttaggcatg ataaaatctt gccggatttg ttttaaagga caaggcgctg
    12301 cacattactt ttgtatcaga agattttcaa tggcaggcaa tcgttagaag ggcaagcgtc
    12361 agaggtcaaa gttaaagcca aaaatcttca aatgctgata taccgataat tatcgggagg
    12421 caacgagagt cagcaacaaa ctctactctt taagaatttc atatggcaag caatcgttag
    12481 aggtcagtcc gtttctacgg aaggaccttt tttgtttaaa ggcctgctaa agtaacaatt
    12541 aattaataca ggcaaaattt ccgtggatat ttttttactt tctctattgc aggatctgtt
    12601 ttgttgaata atcagcatct tacccctcct cttataattc ctgattcttt taccttcaca
    12661 gcataaaata tcatcgctat ggcaatccgc atctttataa ccggcggcac ttttgataag
    12721 gaatacaacg aactggacgg ggaactgtat tttaaagaca cccatatgag cgacctgctc
    12781 gaaatgggcc gaagcaaagt acatgtagaa ataaggactc taatgatggt ggacagcctg
    12841 gagatgacag atgaagaccg ggagcttatc gtacatcaat gcaataactg tgatgaacac
    12901 cagattgtga ttacccacgg caccgacacc atgtctgaaa cagcaaaagt gctggcgaaa
    12961 aaagttaagg ataaaacaat cattcttacc ggggcgatga tacccattaa attcggcagc
    13021 tctgatggct tattcaatct cggcagtgca ttggctttcg cacaaacatt accccccgga
    13081 gtatatgtag cgatgaatgg ccgttatttt actgcagaca atgtgcggaa gaataaacag
    13141 accggtgt
//
LOCUS       DAORTI010000098        13111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4704_length_13111_cov_6.738894, whole genome shotgun sequence.
ACCESSION   DAORTI010000098 DAORTI010000000
VERSION     DAORTI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_13111_cov_6.738894"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLO70_11955"
     CDS             complement(<1..699)
                     /locus_tag="PLO70_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75226.1"
                     /translation="MKKYSSLFAMIILFFVADNAMAQTGKVGINTSVPFTGLHIQDSG
                     VLFNHGFYLFENFPWLDNAQPPVSGAGARLMWFPHRKAFRVGQVDGTQWDRLSTGNLS
                     FATGLNNTAHGRISSVFGRNNTANGDVCFVIGQFNEPVVTAGSNTATAPALIPTAPLF
                     IVGNGDVTNGNSNAMLVRKDGKIGIGTNSPTDRFHINTSASEDAFRVQVNSSTKMRVW
                     NNGGTSIGIASTPPV"
     gene            complement(827..2695)
                     /locus_tag="PLO70_11960"
     CDS             complement(827..2695)
                     /locus_tag="PLO70_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQZ75227.1"
                     /translation="MKKLLAVAIVFIIYLSLSTSHLQAQVGKVGINTSTPQAMLHVKD
                     SSVLFNGGAVIPGAPGNPPVSGAGVRMMWYPDKAAFRAGAVNGLQWNKDSIGNYSFAA
                     GNNTRADGVGSVAMGNNTKALGDYSFAAGNNTLASNYYSVAFGSNTEATGWESSAFGG
                     FSKAWGDYSFATGFGALSKSDYTVSIGLFNDTTEMGVGISSANPLFVIGNGSNGATRS
                     NAFTVLRNAKTGINTARPLAMLHVKDSSVLFTGVSGNLPVLPGDPPASGTGVRMMWYP
                     DKAAFRVGRVSTSVWDKGSIGDYSFAAGFNAKANGDISTAIGFSTIATGTASTAMGSN
                     TEATGNYSTAMGNNTEATGNYSTAMGNNTEATGDLSTAMGLTNTARSFASLSIGRYND
                     SIINSNKNSWITSDPVFIIGNGTADNDRSNALTILKNAKTGINMSDPVALLDIKGLDG
                     TINRHIRLEATSSSNSANIYYNNDLVIQNNSPGGDFYFKDDAGSNVMSLFSTGNMVIA
                     GTLTQNSDARLKKNIQPLQNSLQKILHIGGYQYNWKENYRDKSLQTGLLAQQVEQQMP
                     ELVKEDKEGIKSVNYSGIIPYTVEAIKELKKENDELKAELKELKKIVTGLLLKGDK"
     gene            complement(2799..3446)
                     /locus_tag="PLO70_11965"
     CDS             complement(2799..3446)
                     /locus_tag="PLO70_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75228.1"
                     /translation="MPVKVAIFEDRRSIREAISLLVEGTSGLQLAGAWPDCMNLVENI
                     KSSQPDLVLMDIEMPGINGVEAVKLLKKDFPRLTVVMQTVFEDDEKVFAAICNGASGY
                     LLKDTPPARLMESIVEAYNGGAPMSPSIAKKVLALFQKHLSPTPDAAGEKYNLTNREK
                     DILLGLVNGQSYKMIAEVYHISFDTVRTHVRNIYEKLHVASSTQAVAKAIKERLV"
     gene            complement(3474..6431)
                     /locus_tag="PLO70_11970"
     CDS             complement(3474..6431)
                     /locus_tag="PLO70_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ75229.1"
                     /translation="MKFNLRLIYFFIPLLFFQARVQAQAVIAYEQFTSEQGLSENVVY
                     CLLKDKKGFIWAGTDYGLNRFDGYSFKKYFHNIADSSSLSNQSILSIDEDSKGNFWIV
                     TYKSLNYFNPRTGKADHILLPGKKPDHPVQKIQVLKNGSVLILVDRTEAYIYAHEKKE
                     WKALTFEGGSKLESFDLFQLADKRIGAFCTNIKGTGNALLLLNESLARWEYKELPAIF
                     PFYKEKMPVYYFNSPEGLHFIHSFAEQSLRIYNKAGEEQTSVVFKPGQSNYEFILHDI
                     EKMDSSLYWLATNKGLLIYDAIKKNIIEASLVGDSKSLPGNKELRCLLNDQNGNIWMG
                     IFGEGLLRCTIRRQAFKNIALPDLVGERFLRMIFGLYKWSEEEVAAETEFKNLILIRN
                     GKVSRRLSADELTADDIVRITTGKKMKELSSFQQKILQKWYSKGELLPYRFILKDDTS
                     VISTHLKFALFQPAGEKITSVEQPGNYAEDDEYYWVSSFNGLFRITKKDFSEKIFTYE
                     PGNPYSLSEPSLYHIAMDENKDLWLGTKGGGLNHYDRKQNRFYHYTVNEGLPDNVIYI
                     ILPDKKGNLWLTTNKGISRFNLQTKTFTNFSKRDGLLNAEFNRQGAVLMNDGTIYLSG
                     TSGVDYFNPDDLPATLTQPAVNFSELKINNSEESFDENRSLRFSENNLSFSYTANDYI
                     RPDLVYYRYRLKEKEPWTRVQGVNTVLYNALPAGDYRFEVQSSYDNHTWSQSAVYDFN
                     IQQPWWKNRWFYVALALLTACILYFIYLYRIRQLKKMHQLRTKISQDLHDEVGATLTS
                     ISFLSEVARKQTSDTDAPVVKTLDKIGEYSREMIGEINDIVWAINPANDKFDKITDRM
                     KNFALPLLSAKNIRMEFKADDELMNYSLGMEQRKNLYLVFKEAVNNAAKYAACSQIDV
                     RFEKDKNMLILSITDNGMGFNAAARRNGNGLKSMALRAKEIKAEFQIKSAPGEGTAVL
                     LQMPITKNADKVR"
     gene            complement(6532..8100)
                     /gene="dnaB"
                     /locus_tag="PLO70_11975"
     CDS             complement(6532..8100)
                     /gene="dnaB"
                     /locus_tag="PLO70_11975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQZ75230.1"
                     /translation="MDLTNLNKDRKTRRKSTLDLGTMVYGKVPPQAKDLEEAVLGAIM
                     LEKGAFDTIVEILKPECFYVEAHQRIFSAMQGLANKSQPIDILTVAEELRFREELEMI
                     GGAYYVTKLTNTVVSSANIEAHSRIILQKFIQRELIRISGEIIGDAYEDSTDVFDLLD
                     VAESKLFEITNNNLRKNFETIDSVLVNTIKRIEDLRHKNEDVTGVPSGYPSLDRVTYG
                     WQNTDLIILAARPAVGKTAFALNLARNAVMHPGKQTAVALFSLEMSAGQLVQRILSAE
                     SEIWLEKISRGKMEEHEMKQLYAKGVQRLAQAPLFIDDTPALNIFELRAKCRRLKNKH
                     NIGLIIIDYLQLMSGTGENRNSNREQEISNISRNLKALAKELSVPIIALSQLSRAVET
                     RGAGKDGNKMPQLSDLRESGAIEQDADMVMFLYRPEYYDITTNELGENNRGETHVRIA
                     KHRNGSLETIKLRALLHIQKFTEDDGGTFGAQGLTGNWKPVSDIDGDGGAKVFVQTGS
                     KMNDLPQDDEDTPF"
     gene            8517..9026
                     /locus_tag="PLO70_11980"
     CDS             8517..9026
                     /locus_tag="PLO70_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75231.1"
                     /translation="METKKHSSHSRPVLSQAQNNTTLWIGHLKPHAHDHLAGQTFICP
                     SEGLVNNIQVFASAVTQPGEVALTLHEFDAATKTWGPAIGNSSRNVDKKDGTNWIRFE
                     MEPVNLQKDSTYGFRLQTENGIIGIGEAASGNRHPFTFGHAWNGHGNNDKGEYFRYFS
                     LAFKVELCA"
     gene            complement(9094..10749)
                     /gene="recN"
                     /locus_tag="PLO70_11985"
     CDS             complement(9094..10749)
                     /gene="recN"
                     /locus_tag="PLO70_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQZ75232.1"
                     /translation="MLSRLSIINYAIIDELEIDFSGKLNVITGETGAGKSIIVGALGL
                     ILGERADSAALVNKQKKCVVEGVFKVDSKKSVITFLNGNELDAEEELVVRREIGINGK
                     SRAFINDTPVNLSQLQQLSSLLVDLHQQFDTLEVGETDFQRDVVDALAGHSKLLDEYQ
                     SIYRQWQVAKKECDALKNQKLQFDKEADYNRFQFTELEDAGFKENELEDIDTELKMLS
                     NAEGIKGALSKAYFELKESESPIVQQLKVLLNQLNVYSPYHPDLPVLIQRLQSSQIEL
                     QDIADDVDRISNHINYDPEKIEQLNERLSLGYRLQKKHSVNSTAELLAIQKQLEDKLQ
                     AVLNIDQAIQQKENESTKLITAAISLAKEISGNRKQQVKPLEDKVNKLLVQVGMPNAK
                     LKVEIKPVDLNLSGADAIEFLFDANKSGQFQPVRKVASGGELSRLMLCIKSLVAESVD
                     LPTMIFDEIDTGISGEAARQVGIIMKKLAAKRQVICITHQPQIAGKADAHFFVYKEIV
                     KDAVKTNIRQLTTEERITTIAKMLSGEKPTAAAMENAREMVMN"
     gene            complement(10921..11676)
                     /locus_tag="PLO70_11990"
     CDS             complement(10921..11676)
                     /locus_tag="PLO70_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQZ75233.1"
                     /translation="MAHVKEFSVEEKLTALVTLQKIESKLDELHILKGELPMEVADLE
                     DEITGLHARQTRIEEEINGVTDFIEQRKNAIKEAEALIKKYEKQSENVKNNREFEAIN
                     KEVEMQQLEMKLAEKHIKDASEEIAEKAVLLEKAKKNIAAKEGVLTNKKGELDKIIAA
                     NEKEEKHFAKLATEAKEHVEPRLLASYEKIRHSYRNGLAVVPVERDACGGCFNAIPPQ
                     KQSEIKQHKKIMICENCGRILVDEELSNNVEVK"
     gene            complement(11741..12838)
                     /locus_tag="PLO70_11995"
     CDS             complement(11741..12838)
                     /locus_tag="PLO70_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQZ75234.1"
                     /translation="MKISAIISFLETLAHPSLQEQYDNAGLITGDNQWECTGIICSLD
                     ATEEVVKEAIGKKCNLIVAHHPIIFGGLKKINGKNYVEKTVITAIKNDIAIYAIHTNL
                     DNVKEGVNGKIASMLGLQNLSILSSKENSLRKLFTFVPVDKAEQVRDAIFEAGGGHIG
                     NYSECSFNTEGTGTFKAGQGADPYTGEIGKRHFEKEIKIEVIFPGFLERKIVMAMKAA
                     HPYEEVAYDVVLLSNTHQGIGSGILGELPVSHDEKHFLSLLKEIFKVPVIRHSALLNK
                     SVKKVAICGGAGSFLISKALASEADAYVTADMKYHEFFDANGRMLITDIGHYESEQFT
                     INLLYEVLAEKFPTFAVLKTEVETNPVRYFL"
BASE COUNT         3589 a         2996 c         2397 g         4129 t
ORIGIN      
        1 gaccgggggt gttgaagcaa taccaataga ggtacccccg ttattccata ccctcatttt
       61 tgtactgctg ttcacctgca ccctgaaagc atcttctgat gcagatgtat taatatgaaa
      121 cctgtcagtt ggcgagtttg ttccgattcc aattttacca tctttccgaa ctaacatcgc
      181 attggaattt ccgtttgtaa catcgccatt tcctacaata aataacgggg cagttggtat
      241 taatgcaggc gcagttgcag tattagatcc ggcggttaca acaggttcat taaactggcc
      301 gattacaaaa cagacatccc cattcgctgt attatttctt ccaaacacgg atgatattct
      361 gccgtgagct gtattgttga gccccgttgc gaaagaaaga ttgccggtac ttagcctgtc
      421 ccattgcgtt ccatccacct ggcccacacg aaaggctttg cggtgaggaa accacatcag
      481 tcttgcacct gcaccagata ccggtggctg agcattgtcc agccatggaa aattttcgaa
      541 taaataaaac ccatgattaa acaatacacc actgtcctgg atatgcaagc cggtaaaagg
      601 tactgaagta ttgataccaa ccttacctgt ttgcgccatt gcattatctg caacaaagaa
      661 cagaattatc attgcaaata atgaagaata ttttttcata cctggttttt taagttctca
      721 taaatgtaag atttattaaa taacagttcc gtgtagcaga actattgcag acaattatta
      781 agttactgtt taatacagaa tatggaagaa actgtatctc tgaaatctat ttatcacctt
      841 ttaaaagtaa gcccgtcact atttttttta attctttcaa ttcagctttc aattcatcat
      901 tttccttttt caattctttt atagcttcaa cagtgtaggg aattatacca ctatagttca
      961 cactttttat tccttcttta tcttctttta ccagttcagg catttgttgt tctacctgct
     1021 gggccagcag cccggtttgt aaacttttat ccctgtaatt ttctttccag ttatattgat
     1081 agccgccgat gtgtagaatt ttttgtaaac tgttttgtag cggctgaata tttttcttca
     1141 atcgggcatc gctgttttga gtaagcgttc ctgcaataac catatttcct gtactgaaga
     1201 gtgacataac attacttcct gcatcatctt taaaataaaa atctccaccg gggctgttat
     1261 tctgaattac caggtcattg ttataataaa tattggcact attggatgaa gaagtagcct
     1321 ccagcctgat atgccggttg atagtcccat ccagtccttt tatatccagc aatgctaccg
     1381 ggtctgacat attgataccg gttttggcat ttttcagaat agtcaatgca ttacttctat
     1441 cgttatcagc cgtaccatta ccgatgataa atacagggtc actagtaatc cagctatttt
     1501 tgttactgtt aatgatagaa tcattatatc gtccgattga caaggatgca aatgaccttg
     1561 ctgtatttgt taatcccatt gctgttgata aatctcctgt ggcttccgta ttattaccca
     1621 tcgctgttga ataatttcct gtggcttccg tattattacc catcgctgtt gaataatttc
     1681 ctgttgcttc cgtattacta cccattgctg ttgaggcagt tcctgttgct attgtggaaa
     1741 agcctattgc tgtagaaata tcgccatttg ctttagcatt aaagcctgcg gcaaacgaat
     1801 agtcgccaat actgccttta tcccaaactg aagtagatac ccgcccaaca cgaaatgctg
     1861 ctttatcagg gtaccacatc attcttacac cggtaccgct ggccggcgga tcaccgggta
     1921 atacaggtaa gttaccgctg actcctgtaa ataatacact gctgtctttc acatgcagca
     1981 tagctaatgg tcttgcagta ttaattccgg ttttagcatt tcttaataca gtaaatgcat
     2041 ttgaacgggt tgctccattg ctgccgtttc caattacaaa tagtggattt gcagatgaga
     2101 taccaactcc catttcagta gtatcattaa ataacccgat tgatactgtg taatcagatt
     2161 ttgacaacgc tccaaaaccc gtagcaaaag aataatcacc ccatgcttta ctgaacccac
     2221 caaaagccga cgactcccaa ccagttgctt cggtgttgct tccaaaagct accgaataat
     2281 aatttgatgc caaagtatta tttcccgcag caaatgagta atcacccagt gcttttgtat
     2341 tattacccat agcaacagaa ccaacaccat cagctcttgt attattaccg gcagcgaatg
     2401 aataattacc aatgctgtct ttattccatt gcagtccatt aacagcacct gccctgaatg
     2461 ctgctttatc cgggtaccac atcattctta caccagcacc acttactggt gggtttcccg
     2521 gtgcacccgg aattacagca ccgccattaa ataacacact actgtctttc acatgcagca
     2581 tggcctgagg agtgctggtg ttaattccta ctttgccaac ctgcgcctgt aagtggctgg
     2641 tggaaagtga taaataaata ataaatacaa tagcaacagc tagtagcttt ttcatactca
     2701 ataaactttt tgatagtcaa taggtagtat ggtaaaacta ttcagctaat caaagcggga
     2761 caatcggcaa tttgggtgag ggaaacgagg tgaacctctt aaaccagtct ttcttttatg
     2821 gcttttgcca ccgcttgtgt agaagaagct acatgaagtt tttcataaat attcctgaca
     2881 tgggttcgaa ccgtatcaaa acttatatga tacacttcag caatcatttt ataactttgg
     2941 ccattgacca gcccgagtaa aatatctttt tcccggttgg ttaaattata tttttcaccc
     3001 gccgcatcag gtgtgggaga aagatgtttt tgaaataagg ccaatacttt ttttgcaatg
     3061 gaaggcgaca tcggcgcacc gccgttataa gcctccacaa tactttccat aagccgtgcc
     3121 ggtggtgtat ccttcagtaa gtaccccgaa gccccattgc agatagcagc aaacactttt
     3181 tcatcatctt caaaaacggt ttgcatgact accgtgagcc ggggaaaatc ttttttcaac
     3241 agctttaccg cttctacccc attaataccg ggcatttcaa tatccattaa caccaggtcg
     3301 ggctgtgatg atttaatatt ctccaccagg ttcatacaat caggccaggc accggccagt
     3361 tgtaaacctg aagttccctc tacgagcagg gaaattgctt cccgaataga tctgcggtct
     3421 tcaaatatgg caacttttac tggcattgtg tttttcaaaa ataggtgaat ggatcagcga
     3481 accttatcgg catttttggt gatgggcatc tgcaacagca ctgctgtacc ttcacccggg
     3541 gctgatttaa tttgaaattc cgcttttatt tcttttgccc gcaatgccat actctttaag
     3601 ccattaccat ttctcctagc tgctgcatta aatcccattc cattatcagt aatactgagg
     3661 ataagcatat ttttatcttt ttcaaaccga acatcgatct ggctgcaggc tgcatatttt
     3721 gctgcattgt ttacggcttc cttaaaaacg agatacaggt ttttccgttg ctccatcccc
     3781 aatgaataat tcatcagttc gtcatctgcc ttaaactcca tccggatatt tttggcagag
     3841 agcaatggca atgcgaaatt cttcatccgg tcggttatct tatcaaattt atcattggcg
     3901 gggttgatgg cccagacaat atcattgatc tcgccaatca tttcccttga atactcccct
     3961 attttatcca gtgtttttac aaccggtgca tcggtatctg aagtttgctt tcttgctacc
     4021 tcacttaaaa aagaaatact ggtcaatgtc gcacctactt catcatgcag gtcctgcgaa
     4081 attttggtgc gaagctggtg catctttttc aattgccgta tcctgtacag gtaaataaaa
     4141 taaagtatgc aagctgttaa taatgccagt gccacataaa accagcggtt cttccaccag
     4201 ggttgctgaa tattaaaatc atatacagct gactggctcc aggtgtgatt atcataactc
     4261 gactgtactt caaaacgata atcaccggcg ggcaaagcat tatataaaac ggtgttcaca
     4321 ccctgcaccc tggtccatgg ttccttttct ttcaaccgat acctgtaata cacaagatca
     4381 ggacggatat aatcattggc agtataggaa aaggataaat tattttcact aaaacgcaaa
     4441 gacctgttct catcgaaaga ttcctctgaa ttattaattt ttaattccga gaaattaacg
     4501 gctggttgtg ttaaggtcgc cggcagatca tccgggttaa aatagtcaac acccgatgta
     4561 ccggataaat aaatagttcc gtcgttcatc agcactgcac cctgccggtt aaattcagca
     4621 ttgagtaaac catcccgttt tgaaaagttg gtgaatgttt ttgtctggag attaaacctg
     4681 gaaatgcctt tattggtagt taaccataga tttccctttt tatccggcag gataatatag
     4741 atcacattat cgggcagtcc ctcattcaca gtgtaatgat aaaaacggtt ttgcttacgg
     4801 tcgtaatgat ttaaccctcc ccctttggta cccagccata gatccttgtt ttcatccatg
     4861 gcaatatgat acagcgacgg ttcagaaagc gaataaggat tacccggttc ataggtaaat
     4921 attttctcag agaaatcttt tttagtaatt ctgaataaac cattgaatga ggatacccag
     4981 taatactcgt catcctcggc ataattaccc ggttgttcta cggaagtgat cttttccccg
     5041 gcaggctgaa ataaggcaaa ctttaaatgg gtagaaatta cactggtatc atcttttaaa
     5101 ataaaccggt aaggcaataa ctctcctttt gaataccatt tttgtaaaat tttttgttga
     5161 aacgaactaa gttccttcat tttttttcct gtagtaattc ttactatatc atctgcagta
     5221 agttcatctg ctgatagccg gcgggatacc ttaccatttc tgatgagaat aagattttta
     5281 aactctgttt ctgcagctac ttcctcttcc gaccatttgt ataaaccaaa tatcatccgc
     5341 agaaaacgtt caccaaccag atcgggtaag gcaatatttt taaaggcttg ccggcggata
     5401 gtacaacgca gcaatccttc cccgaaaatc cccatccaga tattaccatt ttgatcattt
     5461 aacaagcaac gtaattcctt atttcccggc aggctctttg aatcacccac cagcgatgct
     5521 tcgatgatat tttttttgat agcatcatag atcaataacc ccttattggt tgccagccag
     5581 tataaggaag aatccatttt ttcaatatcg tgtaaaataa attcataatt tgactgacca
     5641 ggtttaaata caacactggt ctgctcctca cctgccttgt tataaatgcg aagcgattgc
     5701 tcagcaaatg agtgaataaa atgcagccct tccgggctat tgaaataata gaccggcatt
     5761 ttctctttat agaaaggaaa aattgccggc agttctttgt attcccatct ggcaaggctt
     5821 tcattgagta ggagaagggc gttgcctgtc ccttttatgt tagtacagaa tgcaccaatt
     5881 cttttatctg ccagctgaaa aagatcaaag ctttcaagtt tgctaccccc ttcaaatgtc
     5941 aatgccttcc attctttttt ctcatgagca taaatatatg cctccgttct atcaaccagg
     6001 ataaggacag acccgttttt cagtacctga attttttgta ccgggtgatc cggctttttt
     6061 cccggtagca gaatatgatc ggcttttcct gtccgcggat taaaataatt aagggatttg
     6121 taggtaacga tccagaagtt tcccttgcta tcctcatcaa tggataaaat gctttggtta
     6181 gaaagactgc tgctgtctgc aatattgtga aaatatttct tgaaactata accatcgaat
     6241 cggttaagac cataatcagt gccggcccag ataaatcctt ttttatcttt cagaagacaa
     6301 tacaccacat tttctgataa tccttgttca gaagtgaatt gttcataagc aataacagcc
     6361 tgtgcctgca ccctggcctg aaagaaaagg agaggaataa aaaagtatat caaccgcagg
     6421 ttaaacttca tcgggaaagt ttgtcattca atttataaaa gattaatgaa cgggaataaa
     6481 aaaacccgga taaacagatc cgggacttgt atagttggtt aatcttatca atcagaacgg
     6541 tgtatcctca tcatcctgcg gcaggtcatt catcttactg cctgtctgta caaatacttt
     6601 ggcacctccg tcaccatcga tatcactgac gggcttccag ttgcccgtaa gaccctgtgc
     6661 gccgaatgta ccaccgtcat cttcagtgaa tttctgaata tgcaataagg ccctcaactt
     6721 aatagtttcc aaagaaccat tacggtgttt ggcaatcctt acatgggttt caccacggtt
     6781 attctccccc agttcgttgg tggtgatatc ataatattcc gggcgataca ggaacatcac
     6841 catatcagca tcctgctcga tggctcccga ttcgcggagg tcacttaact gcggcatttt
     6901 attaccatcc ttaccagcac cacgggtttc aaccgcacgg cttaactggg acagggcaat
     6961 gatcggtaca ctcagttctt tcgccagtgc tttcagattg cgggagatat tactgatctc
     7021 ctgttcacgg ttactattac ggttttcacc ggtaccgctc atcaattgca agtagtcaat
     7081 aatgatgagg ccgatattat gcttattctt caaccggcgg catttagccc tcaattcaaa
     7141 aatattcagg gccggtgtat catctataaa caatggtgcc tgtgccaggc gctgcacacc
     7201 tttggcatat agttgcttca tttcatgttc ttccatttta ccccggctga tcttctccag
     7261 ccaaatctca ctttctgcag ataagatacg ttgcactaac tgaccggcac tcatctctaa
     7321 agagaataaa gccaccgctg tttgtttacc cggatgcatc actgcatttc tggccaggtt
     7381 cagcgcaaat gctgttttac caaccgcggg ccttgccgct aagatgataa ggtctgtatt
     7441 ttgccaacca taggtaaccc tgtcaagtga ggggtatccg ctgggcacac cagttacatc
     7501 ttcattctta tggcgcaggt cttctatccg tttgatcgta ttaaccagta ccgagtctat
     7561 cgtttcaaaa ttcttccgca ggttattatt ggtaatttca aacaacttac tctcagccac
     7621 atccagcagg tcaaatacat ctgttgaatc ttcataggca tcaccgatga tctcaccact
     7681 gatacgaatc aattcccgtt gaataaattt ttgaaggatg atcctggagt gagcttcaat
     7741 attggccgag gaaacaacag tattggtcaa cttggtgaca tagtaagcac ctccaatcat
     7801 ttccagttct tcccggaaac ggagctcttc agccaccgtt agtatatcaa tcggctggct
     7861 tttattggcc aacccctgca tggcgctgaa gatgcgctgg tgggcctcca cataaaaaca
     7921 ttcaggtttt aatatttcaa caatggtatc gaaagcacct ttctccagca tgatagcacc
     7981 aagcacggct tcttccaggt ctttggcctg cggcggcact tttccatata ccatggtacc
     8041 aaggtcaagg gtagattttc ttctcgtctt gcggtcttta ttcaggtttg tcaggtccat
     8101 tgttcaggta gggcgataaa aggttaaacc atcagttatt cactaatgac caccgggtta
     8161 gttccaaact tgttttaaga aattattact tcttcgttaa aggaatgttt ccgtcagtca
     8221 gtaaggcgaa ataaagagca aaaaagtttt gaaaaaaatt tttctttctg cactattttt
     8281 tcacctcatt tttagggtta tccacatcca catttacccg gccagccacc aacaatttac
     8341 ttaaaaagat tggtaaaatc ccctaaagat tagcacaacc gtgtattact tttatacctg
     8401 attttagtca gtagcacaat attacccgaa accgggagtt acagacatac cagttctttt
     8461 tttaccaata ccgctgcggc cgtaccccta ttcaattatc taaaaactca catattatgg
     8521 aaaccaaaaa acacagcagc cattcccgcc cggtgctaag ccaggcacag aacaacacca
     8581 ctctctggat tggtcattta aagcctcatg cacatgacca tctggcagga caaactttta
     8641 tttgcccctc cgaaggatta gtcaacaaca tccaggtttt tgcctcagcc gtaacacaac
     8701 ccggcgaagt agccctgacc ttgcatgaat ttgatgcagc caccaaaact tggggacctg
     8761 caataggcaa cagcagccgt aacgtagata aaaaagatgg cactaattgg atccgttttg
     8821 aaatggagcc ggtcaatctg caaaaagata gtacctatgg cttccgcttg cagaccgaaa
     8881 acggaattat cggcatcggc gaagccgcca gtggcaaccg gcacccattt acattcggac
     8941 atgcctggaa cggacatggc aacaacgaca aaggagaata tttcaggtat ttcagccttg
     9001 cctttaaggt agagctgtgt gcttagatat aaataataac tgagacggaa taaatgagcc
     9061 tataagattt gtaatcagcc aaatcttata ggtttaattc attaccatct ctcttgcatt
     9121 ctccatagca gcggctgtag gtttttctcc gctgagcatt ttagcgatgg tggtaattct
     9181 ttcttccgtg gttaactgtc ggatatttgt tttcaccgca tcttttacga tttctttata
     9241 tacaaagaaa tgagcatcgg cctttccggc tatctgtggt tgatgggtga tgcagatcac
     9301 ttgtcttttt gcagccagct ttttcataat aataccgacc tgccgtgctg cttccccgga
     9361 aataccagta tcgatctcat caaatatcat agtgggcagg tctacagatt ctgccaccag
     9421 tgatttgata caaagcatca gccggctaag ttcaccccca ctggctactt tgcgtacagg
     9481 ctgaaactga ccactcttat tcgcatcaaa taagaattca atcgcatcag cacccgacag
     9541 gtttaaatca acaggtttta tctccacttt cagtttggca ttgggcatac ctacctgtac
     9601 cagcagtttg ttgactttat cttctaatgg tttcacctgc tgctttcggt tcccggaaat
     9661 ctctttggca agactaattg ctgctgtgat aagttttgtg ctttcatttt ccttttgctg
     9721 aatggcctgg tcaatattca gaacagcctg tagcttatct tctaattgtt tttgtattgc
     9781 cagcagttcc gccgtagaat taacagaatg ctttttttgc agcctgtacc ccagcgaaag
     9841 ccgttcattc agctgttcaa ttttttctgg atcataatta atatgattac tgatgcggtc
     9901 tacatcatcg gcaatatcct gcaattctat ttgtgaagat tgcaaccgtt gaatcagtac
     9961 cggcaggtcc ggatgatagg gcgagtaaac gtttagctgg ttcagcaata ctttcagttg
    10021 ttgtactatt gggctttcac tttctttaag ttcgaaatat gctttactta acgcaccttt
    10081 aatgccttca gcattactca gcatttttaa ctccgtatca atatcttcca gttcattctc
    10141 cttaaatccg gcatcttcca attcggtaaa ctgaaaacgg ttataatcag cttctttatc
    10201 aaactgtaat ttctggtttt tcaatgcatc acattccttt tttgctactt gccattgccg
    10261 gtatatgctt tgatattcat ccagtaattt cgaatggcct gccaatgcat ctactacatc
    10321 tcgctgaaaa tcggtttcgc caacttccag tgtatcaaac tgctgatgca gatcaaccaa
    10381 taatgaactc aattgctgta attgagaaag attaacaggt gtatcattta taaaagccct
    10441 tgactttcca tttattccta tttcccttct caccaccagt tcctcttctg catccagttc
    10501 gtttccattt aaaaatgtaa taacactttt cttactatcc actttaaata caccctccac
    10561 cacacatttt ttttgtttgt ttaccaatgc agctgaatct gccctttctc ccaggatcaa
    10621 acccagtgcc ccaacgataa ttgatttgcc ggcgcctgtt tcgcctgtta ttacattcaa
    10681 cttaccggaa aaatcaattt ccagttcatc tataatagca tagttaatta tggaaagtct
    10741 gctgagcatt tgaaaaagca aattagggat tttaggcaca acttgtcccg catgttggca
    10801 gaatacaaag aattgattgc cggtcgacca gccttaacca acaatagata ctacgttgca
    10861 caaataaaaa aagttctgcc ttccggcaga acttttttat agtatcattt aaattgtcgt
    10921 ctatttaact tccacattat tgctcagttc ttcatccacg agtatacgtc cgcagttttc
    10981 acaaatcata atttttttat gctgtttaat ttcgctttgc ttttgtggcg gaattgcatt
    11041 gaaacaacct ccgcaggcat ctctttctac aggtacaact gccagtccgt tacggtaact
    11101 atggcggatt ttttcgtagc tggccagcag gcggggctct acatgttctt tggcttcagt
    11161 agccagtttt gcaaaatgtt tttcttcttt ttcgtttgca gcgataatct tatccagctc
    11221 tcctttttta ttggtgagaa cgccttcttt agcagcaata ttctttttag ccttctccag
    11281 caaaactgct ttttcagcta tttcttctga tgcgtcttta atgtgtttct ctgccagctt
    11341 catttccagc tgctgcattt caacttcttt attgatagct tcaaactcac gattattttt
    11401 aacgttttcg ctttgcttct cgtatttctt tatcagtgct tcagcttctt tgatagcatt
    11461 tttgcgttgc tcgataaaat ctgtcacccc atttatctcc tcttcaatac gggtttggcg
    11521 ggcatgcaaa ccggttattt catcttccag gtcagctact tccattggca attcgccttt
    11581 cagaatgtgt aactcatcca gtttactttc aatcttttga agagtaacca gggctgtcag
    11641 tttttcttca acggaaaatt ctttaacgtg tgccatttca ggtttgatgt ttgaagtttg
    11701 atattggagg gtaagccagt ctgccgattc ttctaattat ttacaaaaaa taccggacag
    11761 ggttggtttc cacctctgtt ttaaggacgg caaaggtagg gaatttttcg gctaaaactt
    11821 cgtacagcag gttgattgtg aactgttcgc tttcataatg accaatatct gttataagca
    11881 tccggccatt tgcgtcaaag aattcatgat acttcatatc agctgtaaca tatgcatcgg
    11941 cttctgaagc taaagctttg gaaatcaaaa agctgcctgc accaccacaa atggctactt
    12001 tcttaacgga cttatttaac agggcagaat gccggataac tgggacctta aaaatctctt
    12061 ttaaaagaga taaaaaatgt ttctcatcat gagagacggg gagctctccc aggattccgg
    12121 acccaattcc ctggtgggta ttagacagaa ggacaacatc gtaagccacc tcctcgtatg
    12181 gatgagcagc tttcattgcc ataacaattt tccgctccaa aaatcccgga aaaatgacct
    12241 cgatctttat ttccttttca aaatgccttt tcccgatctc tcctgtataa ggatcggcac
    12301 cctgacccgc cttaaaagtt cctgttcctt ctgtattaaa actgcattca ctataattac
    12361 caatatgtcc gcctcctgct tcaaaaatag catcccgtac ctgttcagcc ttatcgacgg
    12421 gtacaaaagt gaagagtttc ctgagtgaat tttcttttga ggaaagaata gatagatttt
    12481 gcaagcccag catcgaagct atcttaccat tcacaccttc ttttacatta tccaggttag
    12541 tatggatagc atagatagca atgtcatttt ttatagcagt gataaccgtt ttctcaacat
    12601 agttcttgcc atttatcttt tttaatcccc caaagatgat gggatgatga gctacaatga
    12661 ggttacattt ttttccaata gcttccttta ccacctcctc ggtagcatcc aatgagcaaa
    12721 taattccggt acattcccat tgattgtcgc cggtaatgag tccggcatta tcatactgct
    12781 cctgcaaaga gggatgagcc agtgtttcaa gaaatgatat gattgcagaa atcttcataa
    12841 gagcaagtta agtatttgaa cgaatctggt aacaggtcat aataactttg ttagcaattc
    12901 ccttatcttt attaactgaa gtcaccattt ctaccctttc gattgctgct tatcctgaaa
    12961 ttattaaaaa aataacatca tgcttcaacg aaccaatggc cgtttactga ttacgttgct
    13021 ggtagttaca ggtttagcag gaactctcaa taataactct gtttctcagc aggaaagaaa
    13081 agtggtttta gagcaactga agacaggcaa a
//
LOCUS       DAORTI010000099        12925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4802_length_12925_cov_5.789821, whole genome shotgun sequence.
ACCESSION   DAORTI010000099 DAORTI010000000
VERSION     DAORTI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4802_length_12925_cov_5.789821"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..1139
                     /locus_tag="PLO70_12000"
     CDS             411..1139
                     /locus_tag="PLO70_12000"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQZ75235.1"
                     /translation="MKIIIKSIITFCLHYLSVGAISAAPGKASPGPEKGIAHTKTTAT
                     DKLKDTSFVNPVIDTSFYRINTTYKAALNRLQVQAAALKEYVAENNFNTEHCFLVDMS
                     IPSGKNRFFIYNLKKDSIELSSLVAHGFGSTRKNSYDELVFSNTPNSFKTSLGKYKIG
                     YAYKGSFGLSYKLYGLDSTNSKAFERTIVLHADQLVPEKEVFPYHIFQSAGCPTVSPS
                     FLGVLTNYIKASRKPILLWIYYSA"
     gene            1252..2679
                     /locus_tag="PLO70_12005"
     CDS             1252..2679
                     /locus_tag="PLO70_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ75236.1"
                     /translation="MKGILTLACFCGLYNCSLSQDVYELTCHLPYDKTQSLYKGFMLR
                     AANGSGFIRLTSTSSQNKINSIYDLELGLNKVYDNEKNRYNEQIIYYPLTRDSFLYGK
                     ARYIRTLAGEANTTFKHLIIWFKKNKTTGKYEPYTGNIPFRVDDRPDQYNGAEFNLVN
                     TTGQQQSTQQTYPVGDKNQLTSYKKLTQNNFERKYLQQFFTALELVNKNQLSATQIVT
                     ARNKHKPVFYLIAVHSEEKLCRKDVTTLSDYCEDLGGFLNIPFKKIVIRENDFNVKKV
                     TQTIQNLTPGSNDIVIFTYSGHGFSYREDEQNKFPQLALWHGDAKNKEFLRANTINLE
                     EIYKMIVAKKARLNIVMGDCCNSYIEMRRQHIIPTKPGVFGPRDKAWNSRAVSDLFLN
                     TRQSYLLAAAQKGELAGSHDWYGGFFTFYFLEAINNKLLDHQSDKPDWNDILKNTGEG
                     ALNRSRDYFCKDAVCGQHMISKTGN"
     gene            2794..3594
                     /locus_tag="PLO70_12010"
     CDS             2794..3594
                     /locus_tag="PLO70_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75237.1"
                     /translation="MKSLFTLVLFFLSTGLIAQPDQGIKQAIAPKNPIAFSYTIKHFG
                     LNGKVKSFNETSDGDPYYKDSLAFDDKGYIKKHVMQRSGRSIEQFKYNLTARQFIMEN
                     IQDGEKLVYTQKLNQQGEVTESSLGKNYKITHLYNNKGLVTEKKETNSKSYTRYEYNE
                     NGQLIREILLNSDTIWSLQSYQYLQENNMLLITLTQELRSTGEKLIYKYTYDSNGLLK
                     NEVNNYAEVNYTYELDKMGNWISMIIDTYDPKTEKTETETWRRVFIYY"
     gene            complement(3749..4537)
                     /locus_tag="PLO70_12015"
     CDS             complement(3749..4537)
                     /locus_tag="PLO70_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75238.1"
                     /translation="MTAVKNVLPVLFFSSFLFFTSCQREVLSTGDQQQDNLTDPVFLV
                     IDEESIDNGNEPNNFSDVNVNDQLAKVGLRQPLDYFQKNIGKTITLFTGQVGDEGLYA
                     IKQIPASWKSTGPTANGARNYLLAGPGLGTGNPDDNKEILLDKIPGITPLRATGLTML
                     KGRTILAVVYDSDISINYSPLEGNLMGANLGIVSFDVLEVTERTDGSTSSLPKITIRI
                     RDANESTKGNLLLFSNAPVPQSSSEPFDIKPPVAVPAIILTPAE"
     gene            4965..5480
                     /locus_tag="PLO70_12020"
     CDS             4965..5480
                     /locus_tag="PLO70_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ75239.1"
                     /translation="MKKLLQSAILAAVVLLSNASYAQEKDVVDIAAGSEAHSTLVAAV
                     KAAGLVETLKGAGPFTVFAPVNDAFGKLPAGTVDFLLKPENKATLVKILTYHVVAGNL
                     DANAVVAAIKAGKGNAVLTTVSGNMLTATIENGKVKLTDEKGGVAFVTVTDLKGSNGI
                     IHVIDTVVLPK"
     gene            complement(5571..6314)
                     /locus_tag="PLO70_12025"
     CDS             complement(5571..6314)
                     /locus_tag="PLO70_12025"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HQZ75240.1"
                     /translation="MTKLSRIFLSARWEYLAMFNYAVDPQILQKHVPPGTEIDYFDGK
                     ALVSIVGFLFNDTRVLGIRWPFHVNFEEANLRYYIKRFDGARWKRGVGFVSEIVPKPL
                     VATMANLFYNEHYSTAKMGHQVVEKPGNLFVEYSWKKRNQYRNVMQVNASPELQDIKA
                     GSEAEFIFEHYFGYNQLTATSTIEYAVAHPRWEIYPVTSFSIDCDIERLYGKEFVPFI
                     TDRQPHSVLLARGSSVTVKMPLKIRGIKK"
     gene            complement(6307..6942)
                     /locus_tag="PLO70_12030"
     CDS             complement(6307..6942)
                     /locus_tag="PLO70_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQZ75241.1"
                     /translation="MLFYNQSEIESWERFYRANFINSLTGFKSVSLVGTADKTGQTNL
                     GLFSSIVHIGSDPALVGYINRPRNAAPHTLANIESTGVYTINHIHPSYLEKAHQASAK
                     YPAEVSEFDEIGLTTEFEKTITAPFVKESRVKYALSLQEIIPIPLNETYLVIGKIEMV
                     KLEDNYVSPDGFIDLHRADTICSNGIDSYYLTQPVGRFQYARPGIKPLRID"
     gene            7068..7244
                     /locus_tag="PLO70_12035"
     CDS             7068..7244
                     /locus_tag="PLO70_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75242.1"
                     /translation="MFSKFKVSELLIIVLVIVLIFISEYYYVVQKDPMRAIFIGLWPP
                     TILGLLIFFNLKRK"
     gene            7253..7525
                     /locus_tag="PLO70_12040"
     CDS             7253..7525
                     /locus_tag="PLO70_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75243.1"
                     /translation="MENLDLIILTTIVVILFMVFILATFKEMQEIAKNPLDTGKERGP
                     RADMIEFVGRIFSEKNIELSEKKELLNIIKKKIDEIEISDNKTSSK"
     gene            complement(7826..8968)
                     /locus_tag="PLO70_12045"
     CDS             complement(7826..8968)
                     /locus_tag="PLO70_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQZ75244.1"
                     /translation="MNRNLIYLSFIFIFFIGCKKGTDDPVIPADPAKLIITSYTTIAA
                     GSGVYNLIAYNADKSVKWSRTGIAPIYPTYPLYHNGLLYVSPDLGPLHALQIETGNNA
                     WTYGTTNYYSTPAVKDNVLYVASSDRKVHAIDAVSGAGLWTLTVNDLVIKTPVMLQNT
                     LFVMSAPVSTAVYSLHAIDINTRAVRWSKVIGVNPPSAVVSGNDCVYLSAANGTLLAL
                     NSIDGSVKWSKPGLSTSNPVLSGNSVYITSASGLYAFDGVTGTQLWHYPVGTFGWGGR
                     NPYIRNNKVYVLNSSEAVLNFESSTGTLIWQKANTESYESPPVVSDDMLIATRQPFQA
                     GGAATTAVMIMDISSGTKKDSILIPPAVNIGAAYILTNAGKWLYPN"
     gene            9202..9711
                     /locus_tag="PLO70_12050"
     CDS             9202..9711
                     /locus_tag="PLO70_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75245.1"
                     /translation="MKQFRIALSLLIAVSFVITACPVGSAYPLAESSAALPFDKALTG
                     TWENFTEDAEASLVTLEKGNTDNTYNVKVLETGDMYGAETKEFTAWQVALNGKRFLVL
                     KENGTSNTSPYYLYHIAVNGSQLITHDVSLLVNGTDAITSVKSYQEELIASMKKEGFL
                     TGKIDWKKK"
     gene            9749..11374
                     /locus_tag="PLO70_12055"
     CDS             9749..11374
                     /locus_tag="PLO70_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75246.1"
                     /translation="MTRKLFIIISLLLTANRNAAQPPVGKCISGDCINGQGIYQLNSF
                     SFYKGGFLNGKFNGEGCIYNPNQWTYTGLFSNGKIEQLRYTGRVNTDTTIQLLFAGKA
                     HLTFYPADEVKANWQKNNFSECNGNYFVVQKDSGRLYNIRGPYDTTRYSFSGRINSEG
                     KLYGVGKIYKGDQVAEYDFGTGYYTFDTEELSRLANNHFTNATADVSNFKCISGDCVN
                     GKGIFQLNKFARYDGGFKNGKMHGPGKLGWEKDNWHITGNFQGNTILNYHYYEQQKGS
                     AAFVLKRKGTGRIKVYDKAEVQKNWTTNIYYYLNNQFFKLEETDTITWFYKPDFFLKV
                     YPDKKKSILISGTKKFGFLLTVDPFEITEDILREHLKRNNYNNKAVYTYDKDIMKKPA
                     TPPVANNQNKPGQKDSRLLNASEYAQLAKIMADEFRNQLSGVVIAEGYMEGGNTNVKY
                     KTIYATVSPGEVYAVLAFVKYSAPVTISVPQNNNPFNNITCKGNRITEAGATVHPYTC
                     NYVNLTYESSRVKFEFNVTNSVEPAYFLVIKVK"
     gene            complement(11411..11881)
                     /locus_tag="PLO70_12060"
     CDS             complement(11411..11881)
                     /locus_tag="PLO70_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HQZ75247.1"
                     /translation="MKHIEKAIDYKVLNISSQSFKEGEMIPVKYTCDGANINPSLDIK
                     GIPDQARSLAIIVDDPDAPAGTWVHWVAWNIPVTQHIKEDHVPGTEGVNDFQQHQYGG
                     PCPPSGTHRYFFKVYALDTILDLPAGTNKMQLEKEISEHIIGFGELTGLYKRNK"
     gene            complement(11899..12117)
                     /locus_tag="PLO70_12065"
     CDS             complement(11899..12117)
                     /locus_tag="PLO70_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75248.1"
                     /translation="MPEKFIKVNAMAIKKKPADPQSGFPYPGKRPEIDPDTVPDNPVI
                     PGEEPDIIPDDDPFETPPEDVPPPGEKP"
     gene            12276..12803
                     /locus_tag="PLO70_12070"
     CDS             12276..12803
                     /locus_tag="PLO70_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HQZ75249.1"
                     /translation="MKEIITPSITRLHDLLDYDARKFTNAEIQLKNSLPGWINTANSV
                     KLKEVLLKYMDYVDEHIEKLDDFFIAEEINSLSTKNRVMHALIEEIGEKLNNCTDKEV
                     KDACLLAGIQEINHFKISSYGTAAAFAKTLNMEKAADIFHGAEVNEKQIDERLSQLAK
                     FEVNKKARSPISLPE"
BASE COUNT         4253 a         2563 c         2451 g         3658 t
ORIGIN      
        1 gcattttgta atgggtttgt gtaaaaacaa attgcgcaaa tgtagggcag aaaaggtgat
       61 tttaaggaag gaattttaaa gcagttatac acagaatggc tgcctgtaat accctccggt
      121 aacctataag gatggtgaaa catcccatac aaaacagtca ctttgtacat tgaccgccat
      181 caatattttc attagaatga aatgcaagat ataacgcata aactcctgaa tcataagtaa
      241 caatcccatg aacaccaaag caccaacggc tgcccgccac gtcaaacaaa atatattaac
      301 aagacatata taaaaaactg aagtagcttt atattctgtc agcttttcgg ctactatctt
      361 ttctaagatt catattccgt tatatttttt aatcatagtt ccggggaata atgaaaatta
      421 tcatcaaatc cattatcact ttttgtcttc actacttatc tgtgggtgct atcagtgctg
      481 ctcccggaaa agcttccccc ggtccggaaa aaggaattgc ccatactaag acaaccgcaa
      541 ctgataaatt aaaagacacc agctttgtca atccggttat tgatacttcc ttttaccgaa
      601 tcaacactac ttataaagcg gcacttaacc gtttacaagt acaggctgct gcattgaaag
      661 aatatgtagc cgaaaataat tttaatacag aacattgttt cctggtagat atgtccatcc
      721 cttccgggaa aaaccgcttc tttatttaca atctgaaaaa ggattcaatt gaactgtctt
      781 cgttggtggc ccatggattt ggttcaacca ggaagaatag ttatgatgaa ctggtgtttt
      841 ccaatacacc caatagtttt aaaacctcat tggggaaata caaaatagga tatgcttata
      901 aaggcagttt cgggttgtcg tataaattgt atggattaga cagtactaat agcaaagcct
      961 ttgaacggac catcgtttta catgccgatc agcttgttcc ggaaaaagaa gtctttccat
     1021 atcatatatt tcaaagtgcc ggttgcccaa cagtttcgcc ttccttcctc ggtgtactga
     1081 ccaattacat caaagcttcc aggaagccca tcctgttgtg gatctattat tctgcgtaat
     1141 tgcccaacat ttattctttc gtctttcccc tttttacctg tgaatctgga aattaattca
     1201 aggctgtaaa cagtcaaaat cataccttca aagagaatca aaaaccagaa attgaagggc
     1261 atacttacac tggcatgctt ttgcggtttg tataactgtt ccctgtcgca ggatgtatat
     1321 gaattaacct gccaccttcc gtacgataaa acacaatcac tttataaagg tttcatgctc
     1381 cgggcagcaa atggcagcgg ctttatccgg ttgacaagta ccagttccca aaataaaata
     1441 aatagcattt acgatcttga actggggctg aacaaagttt acgataacga aaaaaataga
     1501 tataacgaac aaatcattta ttacccgctt acccgtgata gttttttata cggtaaagcc
     1561 aggtatataa gaacattagc aggagaagca aatactactt ttaaacacct catcatttgg
     1621 tttaaaaaaa ataagaccac cggtaagtat gaaccttata ctggcaatat cccctttagg
     1681 gttgatgaca gacccgatca gtataatggt gctgaattta atctggttaa tacaactggc
     1741 caacaacaaa gcacccagca aacatacccg gtaggtgata aaaaccaact aaccagttac
     1801 aagaagctta cacagaataa ttttgaaaga aaataccttc agcagttttt tactgcattg
     1861 gaattagtta ataaaaacca gctttcagct acacaaatag taacagcccg caataaacac
     1921 aaaccggttt tctatctgat cgccgttcat tcagaggaaa aactttgccg taaagatgta
     1981 accaccctct ccgattattg tgaagatctg ggcggttttc tgaatatccc ctttaaaaag
     2041 atcgttatca gggagaatga ttttaatgta aaaaaagtca cccaaactat ccagaacctt
     2101 acacccggca gcaatgatat tgttatattc acttattcag gacatggctt cagttatagg
     2161 gaagatgaac aaaataaatt cccccagtta gccctctggc atggtgatgc aaaaaacaag
     2221 gaattcttaa gggccaatac aatcaatctt gaggaaatct ataaaatgat cgtggcaaaa
     2281 aaagcaaggt taaatattgt gatgggtgat tgctgcaata gttatattga aatgagacga
     2341 cagcatatca tacctaccaa acccggtgtg ttcggaccac gagacaaagc atggaatagc
     2401 agggcggttt cagatctatt cctcaacacc cgtcaatcgt atttattggc tgctgctcaa
     2461 aaaggagaac tggcaggctc acatgactgg tatggcggtt tctttacttt ttatttcctg
     2521 gaagccatta ataataaatt acttgaccat cagtctgaca aacccgactg gaatgatatt
     2581 ttaaaaaata caggagaagg agccctgaac cggagcaggg attatttttg taaagacgca
     2641 gtgtgcggac aacatatgat ttctaaaacc gggaactaaa tcttttgaag gcaatagctg
     2701 attaacagac aaccactttc aaaaactacc tttctccatc tattttcatt tttcctgatt
     2761 tatactattt ttaagtcaat aacaggacaa aaaatgaaat cattatttac tcttgtattg
     2821 tttttcttgt caacgggcct tatagcgcaa ccagatcaag gaattaaaca agccattgcc
     2881 ccaaaaaatc ctatagcgtt cagttacact atcaaacatt tcggactgaa tggaaaagta
     2941 aaatctttca atgaaacaag tgatggcgac ccctattata aagacagcct tgcctttgat
     3001 gataaagggt atatcaaaaa acatgtcatg cagagatccg gccgtagtat tgagcaattc
     3061 aaatataatt taactgcccg ccagttcatt atggaaaata tacaggacgg tgagaaactg
     3121 gtttatacgc aaaagctgaa ccagcagggt gaagtaacag agtcaagcct cggaaaaaat
     3181 tataaaataa cccacctgta caataacaaa gggctagtga ctgagaaaaa agaaactaac
     3241 agcaaaagct atacccggta tgaatacaac gaaaacgggc agctgatccg ggaaatacta
     3301 ttgaatagtg atacaatttg gtctttgcaa tcttaccagt acctgcagga gaataatatg
     3361 ctgctgatca cattaacaca ggaattgagg tcaacaggag aaaagctgat ctacaaatac
     3421 acttatgata gtaatggcct gctgaaaaac gaagtaaata attatgcaga ggtcaattat
     3481 acgtatgagc tggacaaaat gggtaattgg atcagtatga tcattgatac ttatgaccca
     3541 aaaacagaaa agacagaaac ggaaacttgg agacgggtat ttatctatta ttaaatagta
     3601 agggattata tcgctttcct gaaaataaat gttggcagaa taactgcagc actcaaacag
     3661 tgtaaaagaa aggcccgggt aatattgccc gggcctttct tcttattgcg aagaatacat
     3721 tattaaactc ttattcatga gcataagctc actctgcagg agtcagtata attgctggta
     3781 ctgcaacagg tggctttata tcaaatggtt cagatgaaga ttgtggtacc ggtgcatttg
     3841 aaaaaaggag caggttacct ttagtagatt cattggcatc ccggatacga attgtgatct
     3901 ttggcagtga ggaggtagaa ccatcagtac gttctgtcac ttccagcaca tcaaaagata
     3961 caataccaag gtttgcaccc atcaggtttc cctccagtgg tgaataattg atgcttatat
     4021 cactgtcata tacaacagcc aatattgttc ttccttttag cattgtcaac cctgtagcac
     4081 ggagaggagt tataccaggt atcttgtcca gcaatatttc tttattatca tcaggattgc
     4141 ctgtacctaa acctggccct gccagtaaat aattcctggc tccgtttgct gtaggaccgg
     4201 tactcttcca gctggcagga atttgtttga tcgcatacag cccctcatca cctacctgac
     4261 cggtaaataa ggtgatcgtt ttcccgatat ttttctgaaa ataatccaat ggttgtctta
     4321 aacccacctt tgccaactgg tcattcacat tgacatcgga aaaattattt ggctcattac
     4381 cattatcaat gctctcttca tcaattacta ggaatacagg atctgtcagg ttatcctgtt
     4441 gttgatctcc ggtagaaagc acttcccgct ggcaggaggt gaaaaaaaga aacgaagaaa
     4501 aaaacagaac cggcaataca tttttaactg ctgtcatact tattaatttt tgtttcagga
     4561 ataaagaacg attaaagttc gatgctatta gaaagcacta tttcttccca taaatcatac
     4621 caacagtaag tattcctgaa agtaattatc aacagtttct tactcgttta aaatatgcct
     4681 caaaaatcaa tagacaactt caacaaacct tataaattat tttataccgc tgaataaagg
     4741 cacttcaaac ggaaagaaag atatgggaat gcttaattgt ggatgataat gaaaaacagc
     4801 agtattgtaa aaatcatctt gtttaaagat ttcaatggta acagataata aaataaacaa
     4861 ataaaagtat tatccaactt ttgtccacat caagaaccat tttcattcag cgaggttatt
     4921 ctttaattca aatcaaataa ataaataaat aaaaaaaaag actaatgaaa aaattattac
     4981 aatctgccat cctggctgca gttgtcctat tatcaaatgc ttcttacgca caagaaaaag
     5041 atgtcgttga tatcgcagca ggttctgaag cacattcaac actggtagcg gccgtaaaag
     5101 cagctggcct ggttgaaaca ttaaaaggcg caggtccttt caccgtattt gcaccagtaa
     5161 acgatgcatt tgggaaattg cctgcaggaa cagttgattt tttactgaag ccggaaaata
     5221 aagcaacact ggtaaaaatt cttacttacc atgtagttgc tggcaacctt gatgcaaatg
     5281 cagttgtagc agccattaag gccggaaaag gtaatgctgt tttaacaaca gttagtggca
     5341 atatgctaac tgctacgatt gaaaacggca aagtaaaatt aacagatgaa aaaggcggtg
     5401 tagcgtttgt aactgttacg gaccttaaag gaagtaatgg tatcatccat gttattgata
     5461 cagttgtact cccaaagtaa taggtagatt tataactgaa aaaagctcct tgatcaagga
     5521 gctttttttt gtaaatagtt caaaataaaa ttagcttctg tttaaacctg ctacttttta
     5581 atccccctga tttttaaagg catttttacc gttacagaag acccccttgc aagtaaaaca
     5641 gagtgaggct gcctgtcagt aatgaatggt acaaactcct tcccgtagag ccgctcaata
     5701 tcacaatcaa tggaaaagct ggtcaccgga taaatttccc agcggggatg tgccaccgca
     5761 tactcaattg tactggttgc tgttaattga ttatacccaa aataatgttc aaaaatgaat
     5821 tctgcttctg aacctgcttt aatatcctgt agttcaggcg atgcattaac ctgcatcaca
     5881 ttccggtatt gatttctttt tttccagcta tactcaacaa ataaattacc cggtttttcc
     5941 actacctgat gtcccatttt tgctgtactg taatgttcat tatagaataa attagccatg
     6001 gtggctacaa ggggtttggg tacaatctct gaaacaaacc ctactcccct tttccaccgg
     6061 gcaccatcaa atcgtttaat ataatatcgc aggtttgctt cttcaaaatt tacatgaaaa
     6121 ggccagcgga tccctaacac cctggtatca ttaaataaaa aaccaacaat acttaccagt
     6181 gccttaccgt caaagtaatc aatctctgta ccaggcggca catgcttttg cagaatttgc
     6241 ggatctaccg catagttgaa catagccagg tactcccatc gggcagataa aaatatgcgg
     6301 gataatttag tcaattcgta atggtttgat acctggtctt gcatactgga atcggccaac
     6361 aggctgagtt aaataataac tgtcaatacc gttactgcaa attgtatctg ccctgtgaag
     6421 gtcaataaaa ccatccggag aaacataatt atcttccagt ttaaccatct caatttttcc
     6481 aatcactaaa taagtttcat tcagtggaat gggaatgatc tcctggaggg ataatgcata
     6541 ttttacccgg ctctctttta caaaaggagc agtaatagtt ttctcaaatt cagttgttaa
     6601 tcctatttcg tcaaattcac ttacttcggc agggtacttt gcactggcct ggtgtgcttt
     6661 ttccaggtaa gacggatgta tatgattaat ggtgtacaca ccggttgatt cgatattggc
     6721 cagtgtatgc ggagccgcat tccgcggacg gttgatataa ccgaccaggg caggatcgga
     6781 gccaatatgt acaatactac tgaaaagacc gaggttggtt tgaccagttt tgtctgctgt
     6841 acccacgagg cttactgact taaacccggt aaggctgtta ataaaattag cccggtaaaa
     6901 ccgttcccag gattcaattt cagattgatt gtaaaacagc ataaaagaaa ttatagtaaa
     6961 cattaaacaa aatccacaaa aaaatgttgt actaaaagtc ttattaattc ataatcattt
     7021 ctgcaatttt tcaaattccg ttgatttaat gaaataaatt aaaaactatg ttctccaaat
     7081 ttaaagtatc agaattactg attattgtgc tggtcattgt attaattttt atatcagagt
     7141 actattatgt agttcagaag gatccgatga gagcaatttt tatcgggtta tggcctccta
     7201 caatactagg tttactgatc ttcttcaatc ttaaaagaaa ataagcatta acatggaaaa
     7261 tttagatctc atcatactga caacgattgt tgtgattcta ttcatggttt ttatactcgc
     7321 cacttttaag gaaatgcagg agattgcaaa aaacccatta gatactggaa aagaaagagg
     7381 accccgtgcc gacatgatag agtttgttgg cagaattttc tctgagaaaa atattgaact
     7441 cagcgaaaaa aaagaacttt taaatatcat taagaaaaaa attgatgaaa ttgaaataag
     7501 tgataataaa accagtagta aataaaatca atgctattcg atgctgtaca actttgaaat
     7561 aaatattttt atcaaaaaaa gtctttacag caatttgtaa ttaccctgac tgccgggtag
     7621 cttctaatta agtgtgttag cctcctgtaa caaacattat cttattccgc agaataatga
     7681 caggcaaaat ttccatttgg tatcgttaaa gataaaaatg aaaactttct atatctaccc
     7741 aaaaattcat gacttctttt attgtcccgt caacgaaaca gaggacaaaa gtccttatgc
     7801 tgcattcatg aaaaaataat attacctaat taggataaag ccatttgcct gcatttgtca
     7861 gaatataggc tgcccctatg ttaacagctg gcgggataag tatagaatcc ttttttgtac
     7921 ctgaactgat atccattatc atcacagctg tggtggcggc tccgcccgcc tggaaaggct
     7981 gacgggttgc aataagcatg tcatcactca caacaggcgg agattcgtag gattctgtat
     8041 ttgctttttg ccaaattaaa gtgccggttg aagattcaaa atttaaaaca gcctcggaag
     8101 aattaagtac gtaaactttg ttattcctga tataaggatt tcttccaccc catccgaaag
     8161 tacccaccgg atagtgccaa agctgcgtac cggttactcc atcgaaagca tataagcctg
     8221 atgcacttgt aatataaact gaattgccag agagtacagg attagaagtt gaaagaccag
     8281 gtttagacca tttgactgaa ccatcaatac tattgagagc cagtaatgtg ccgttggctg
     8341 cagacaggta tacacaatca ttacctgaca ccacagcaga aggtggattt acaccgatta
     8401 cttttgacca tcttactgcc cgggtattaa tatcaatggc atgcaggcta tatacagcag
     8461 tggaaacagg agcactcatt acaaacaggg tgttttgcag catgaccggc gttttgatta
     8521 caaggtcatt aacagtcaat gtccagagtc ctgcacctga aactgcatca atagcatgca
     8581 ctttcctgtc actggatgcc acatacaata cattgtcttt tacagcaggc gtggaataat
     8641 aattggtcgt accataggtc caggcattat taccggtttc tatttgaagg gcatgtaatg
     8701 gacccaggtc tggtgataca tataataacc cattatggta aagcggatac gtgggataga
     8761 tgggagcaat acccgtccgg ctccatttta cagatttgtc agcgttatag gcaataagat
     8821 tatagacccc gctaccagct gctattgtag tatacgatgt aatgatcagc ttggccgggt
     8881 ctgccggaat taccgggtca tctgttccct ttttacaacc aataaaaaaa ataaaaatga
     8941 atgacagata gataaggttc ctgttcataa tagggcgtta gcaataaaaa taataaatta
     9001 cccgcatatc taaaactgct ttgaacaatt gattactgta ccaggtaatt aggctggaat
     9061 gatgttgctt aaatagcacc ccgatacttc tctttataca agaacttaac gaaaaatatc
     9121 gcataaaata ccatgaagag ggtacactga aattactgta attaattagt ttcgctgtgt
     9181 ccctaacaaa aagatatttt tatgaaacaa ttccgtatcg ctttatcctt gctgatcgct
     9241 gtatcctttg tcatcacggc ttgccctgtt ggcagtgctt atcccctcgc tgagtcctcc
     9301 gctgccctgc cttttgataa agccttgacc ggcacctggg aaaattttac cgaagatgct
     9361 gaggcatccc ttgttacact ggaaaaagga aacacagaca atacctataa tgtgaaagtg
     9421 ctggaaaccg gtgatatgta cggagcagaa acaaaagaat ttacagcctg gcaggtagcc
     9481 ctgaatggta aaagattcct tgtactgaaa gaaaacggca ccagtaatac aagtccttat
     9541 tacctgtacc atattgcagt gaatggcagt caactcatca cccatgatgt cagcctgctt
     9601 gtcaacggaa ctgatgccat tacttctgtg aaaagttacc aggaagaatt gatcgcttcg
     9661 atgaaaaaag aagggtttct taccggtaaa attgactgga agaaaaaata aaccaggcaa
     9721 taaattatat ttagataaac aaaattgtat gacccgcaag ctgtttatca tcatctccct
     9781 gttgttaact gcaaatcgga atgcagcgca gccccccgtt ggtaaatgta taagtggtga
     9841 ttgcattaat ggccagggta tttaccagct caacagcttt tcgttttaca aaggaggatt
     9901 cttaaacgga aaatttaatg gcgaaggttg tatttacaac cctaaccaat ggacctacac
     9961 cggccttttc agtaatggca aaattgaaca actacgctat accggaagag taaacacaga
    10021 cacaacgata caattacttt ttgccggtaa agcccattta actttttatc cggcggatga
    10081 agtaaaagca aactggcaaa aaaataattt cagtgaatgc aacggcaatt actttgtcgt
    10141 gcagaaagac agtggccgcc tctataatat ccgtggtcca tatgatacga cccggtattc
    10201 attttcaggc aggataaata gtgaaggtaa actatatggt gtcggtaaga tatataaagg
    10261 agaccaggtt gcggagtatg acttcggcac cggttattat acgtttgaca cagaagagct
    10321 gagccgttta gccaataatc attttaccaa tgctactgca gatgtcagca acttcaaatg
    10381 catcagcggt gattgtgtca atggtaaagg gattttccaa ctcaacaaat ttgcccgtta
    10441 tgatggagga tttaaaaacg ggaaaatgca tggccccgga aaactgggct gggaaaaaga
    10501 caactggcat attaccggta atttccaggg caataccatt ctcaattatc actactatga
    10561 acagcaaaaa ggctcagcag cttttgtatt gaaacgcaaa ggaaccggcc gcataaaagt
    10621 ttatgataaa gcagaagtac aaaaaaactg gactaccaat atttattact acctcaacaa
    10681 tcaatttttt aaactggaag aaacggatac cattacctgg ttctacaaac cggatttttt
    10741 cctgaaagta taccctgata agaaaaaatc catccttatt tccggtacta aaaagtttgg
    10801 gttcctgttg accgtagacc catttgaaat tacagaagat atcctaagag aacacttgaa
    10861 aagaaacaac tataataaca aggccgttta tacttatgat aaggatatta tgaaaaaacc
    10921 cgcaacgcca cctgttgcaa acaatcaaaa taaaccggga caaaaggaca gtcgattgct
    10981 gaatgccagc gaatatgccc aactggccaa aataatggcg gatgagttca ggaatcaatt
    11041 atccggggta gtcattgcag aaggatatat ggagggcggt aatacgaatg ttaaatacaa
    11101 aactatttat gctactgttt cacctggtga agtatatgca gttttggctt ttgtaaaata
    11161 ctcagcaccg gtcactattt cagttccgca gaataataac cctttcaata atattacttg
    11221 taaaggtaac cgcatcactg aagctggggc tactgtacac ccttacacct gcaactatgt
    11281 taacctaacc tatgaaagca gccgggtaaa atttgaattt aatgtaacca actctgtgga
    11341 gccggcttat tttttggtaa taaaggttaa ataagaaaac aattacaaat taaataaccg
    11401 gcagtacttc ttatttattt cgcttgtata acccggtcaa ttctccaaaa ccaataatat
    11461 gctcacttat ctctttttcc aattgcattt tattggtgcc ggcaggcaaa tctaaaatgg
    11521 tatcaagcgc atatacttta aaaaaatagc gatgggtgcc cgaaggaggg catggcccac
    11581 cgtattggtg ttgctgaaaa tcatttaccc cttctgttcc cggaacatga tcctctttga
    11641 tgtgctgtgt taccggaata ttccaggcca cccagtgcac ccaggtgccg gcaggagcat
    11701 ccgggtcatc aactattata gcaaggctcc tggcttgatc cggaattccc tttatatcta
    11761 aggatggatt gatgttggct ccgtcacatg tgtattttac cggtatcatc tccccttctt
    11821 taaaagattg actggaaata ttcagcactt tataatcaat ggctttttca atatgtttca
    11881 tatccgctgt atttattgct atggcttttc acctggaggc ggaacatctt caggaggtgt
    11941 ttcaaaaggg tcatcatcag ggataatatc cggctcttct ccgggaatca caggattatc
    12001 gggaacagta tccgggtcaa tctcgggacg ttttccgggg taaggaaatc ccgactgagg
    12061 atccgctggt ttctttttga tagccatagc attcacttta ataaatttct ccggcatcgt
    12121 ttcacaccgt ttaccattac gttaaatctg aatcaataaa agtaaatcta accccaccgc
    12181 ggtacctatg agagcactca gcaaaaacaa tgacattggt cacaacaatc cgcctgccag
    12241 gcagtttaaa ttacaggaag taaaatctat ggcatatgaa agagataata accccctcca
    12301 tcaccaggct gcatgacctg ctggactatg atgcccgcaa gtttacaaat gcagagatcc
    12361 agttaaaaaa cagcttaccc ggctggatta acacagcaaa ttctgtaaag ttgaaagaag
    12421 tgttgttgaa atacatggat tatgttgatg agcatattga aaaactggat gacttcttta
    12481 tcgcagagga aattaattcg ttaagtacaa agaacagggt catgcatgcc ctgatagaag
    12541 agattggtga aaagctgaat aactgcaccg acaaagaagt aaaagatgcc tgcctgctgg
    12601 cgggtatcca ggaaatcaat cattttaaaa taagctccta tggcacagct gctgcttttg
    12661 caaaaaccct gaatatggaa aaagcagcgg atatcttcca cggagcggaa gtaaatgaaa
    12721 aacagatcga tgaacggctg tcacaactgg cgaaatttga agtaaataaa aaagcaaggt
    12781 ctcctatttc attgcctgaa taaaacaggg gttcaggtta tttcaggatg cttttttata
    12841 gttataccca tgcccctttt tgtttacttt tacaggcctg cggaaaacag ggttaaagca
    12901 tgataaaatt cgtactctgt ttttt
//
LOCUS       DAORTI010000100        12689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_4941_length_12689_cov_6.866946, whole genome shotgun sequence.
ACCESSION   DAORTI010000100 DAORTI010000000
VERSION     DAORTI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4941_length_12689_cov_6.866946"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLO70_12075"
     CDS             <1..410
                     /locus_tag="PLO70_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75250.1"
                     /translation="QGYKEKLFSDPDIMKGEEKNKSEFATAFLQGIGKQSSLTSKGIT
                     TETLTMIRTRFILDWYENNAVKYPFRLFEYHRQLLQEGMFNAYNQWLFGASENLAAYD
                     NWTKAHAEEYNGFTTFQKSRIFKMPQGQYYQVN"
     gene            544..2325
                     /locus_tag="PLO70_12080"
     CDS             544..2325
                     /locus_tag="PLO70_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQZ75251.1"
                     /translation="MTEAKRLFDCIEYHLERKPLEDMLAGKEGGQWKKYSTAAVKDTV
                     DKLSAGLLSLGISCGDMSAEGRDKVAILCKNRPEWVMLDLAVQQIGAVLTPIYPTINV
                     NELEFVLNDAQVKTVFVNDEELFLKVLSLKEKVHSLKDIYTFEHVPNAKHWKEVTALA
                     TPELIAQIKPVADRITYEDLATIIYTSGTTGTPKGVMLSHRNILSNVMASMPCFPPGD
                     EMRSLSFLPLNHIFERMVTYLYLFRGTSIYYAESLDTIGENLKEVQPHMFTTVPRLLE
                     KVYDRIMQKGNELTGTKKKLFFWAHSLAEKFEINKDLGALYNFKLALANKIIFSKWRE
                     GLGGNIKCIVTGGAACQVRLIRIFTAARITIMEGYGLTETSPVVAVNRYEESGRGFGT
                     VGPLIEGVEVKIAEDGEILTRGPNVMMGYYKRPDLTAEVIQDDWFSTGDIGTMTENNF
                     LKITDRKKELFKTSGGKYVAPLPIENKLKESPFIEQVMLVGADRKFVSALMVPSFPNL
                     FDWCRKNGITETSHEEVIRNPKVIDLYKELVESFNKFFNHVEQVKKFELLPNEWSVDT
                     GEMTPKLSLKRKVVIEKYRDAIERIYS"
     gene            complement(2328..2810)
                     /locus_tag="PLO70_12085"
     CDS             complement(2328..2810)
                     /locus_tag="PLO70_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HQZ75252.1"
                     /translation="MKNSSNKILSVAVVLLLITNIGLVIFLMNGKGRNDGKRTGGGKG
                     DPSEMMAKELGMTEQQIKEHKQLKEEHFKTIKPFYDSLRAARSAFYALIKDAGVSDST
                     IAAYSQRINEKQAEIDKLTFNHFKRVRNLFTPEQQPKFDEFVQKMMQRSKKDSAAKGK
                     "
     gene            complement(2811..3146)
                     /locus_tag="PLO70_12090"
     CDS             complement(2811..3146)
                     /locus_tag="PLO70_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75253.1"
                     /translation="MNTEKNNKMEEILNSLDGCQRVPAPDFFYTRLKAKMEKGLEPAS
                     PRSWILRPVFVIAALLVVLLINAVVVFDNDNSSFIENASFDTETIQSIAAEYRLNDNG
                     ILYDLNKDK"
     gene            complement(3199..3762)
                     /locus_tag="PLO70_12095"
     CDS             complement(3199..3762)
                     /locus_tag="PLO70_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ75254.1"
                     /translation="MTEFELIQGLRNGDEAAFKYLVDTYKDRVFNTAIGIVQNAEDAE
                     DVAQEVFIQVFRSVQNFKAESKLSTWIYRITTTRALDLLRSRKSKKRFGIIQRLFGEG
                     NEPEMEIPDFNHPGVALDRKENAARLFKAIGQLPENQKIAFTLHKLEDLSYQEVSEVM
                     GTSVAAVESLLHRAKQNLKKILEKEMG"
     gene            3852..3923
                     /locus_tag="PLO70_12100"
     tRNA            3852..3923
                     /locus_tag="PLO70_12100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3885..3887,aa:Arg,seq:cct)
     gene            3946..4596
                     /locus_tag="PLO70_12105"
     CDS             3946..4596
                     /locus_tag="PLO70_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75255.1"
                     /translation="MKFYNLLIKYRLWIGIALIGLGIYVNYASSFWPAFPLYLVGVIL
                     VAGHFFFGPLRLIQEYMETGDMDGAEKVLASIKYPNLLYKPIRSVYFTLKGNIAMMKQ
                     DFDGAEKMMKKGLDLGMPMKEAEGASLLQMGMLSMQKNDLKQAESYIRQAIRKGLPDK
                     ENEAAAYLQMCSLMMNKREFRAAKDFFRKTKALKPTTPQIVDQIKQIEKYISRIPG"
     gene            4695..6614
                     /locus_tag="PLO70_12110"
     CDS             4695..6614
                     /locus_tag="PLO70_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQZ75256.1"
                     /translation="MKKVHLLTIITFLFSSVYSQKAGHKNEIISIIPQPVSIIQEKGS
                     FTLPYNIVIITPKNDEVKRIAQHLSRQIKTVTNQVTIKEGTSAIPRSILLSLTGNKNI
                     PKDGYQLKITTAGITLLANEPSGIFYGVQTLLQLFSKEIESTVIHQKPDAWVLPIATI
                     KDHPRFGWRGLMLDVSRHFFTVAEVKNYIDQMVKYKFNLLHLHLTDDQGWRIQIKSLP
                     KLTEVGAWRVERTGTFGTLSKPTPGEAVTYGGFYTHEDIKEIVKYAGDRFVNILPEID
                     VPGHSLAAIASYPELSCTPGEYYVSPGDRFMIWPGGGQHFYGLLDNTICPALEKSFEF
                     LDKVFTEVAQLFPFEYIHMGGDETARNFWEKSEQIKALMQKENLKNLDEVQSYFVKRV
                     EKIINSKGKKMIGWDEILDGGLAPNAAVMSWRGMKGGIEAAKQGHEVVMSPTDFAYID
                     YMQGDASIEPPVYSTLRLKKAYQFEPVPEGVNASLIKGGQANLWTEQVYNMRHAQYMT
                     WPRALAIAEALWSPKDKRNWNDFATRVETHFGRLNAAQVKYAPSIYDPIFEITKKDSA
                     TIIIKLSTEIEGLSIHYSFDNSFPDNYYPVYSSPLIVPKDASALKVITYRDNKPIGRL
                     IVMPIAEMRKRAGIK"
     gene            complement(6630..7103)
                     /locus_tag="PLO70_12115"
     CDS             complement(6630..7103)
                     /locus_tag="PLO70_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQZ75257.1"
                     /translation="MAAHSIKTVQKIPISLERAWGFFSNPANLQAITPGDMGFKIVSR
                     HHGDKMYAGQIIEYTVKPLLGIPVYWMTEITQVKDKEYFIDEQRFGPYSLWHHQHHFK
                     EIPGGVEMTDIIHYKNPAWFFGKMANTLFVKKKLEGIFEYRYKKVEELFGVWSTQ"
     gene            7166..10318
                     /locus_tag="PLO70_12120"
     CDS             7166..10318
                     /locus_tag="PLO70_12120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQZ75258.1"
                     /translation="MSFNKERLERKFAEEYERLNEKQKLAVNTIDGPVMVIAGPGTGK
                     TQILASRIGKILLETDALPENILCLTYTDAGVVAMRKRLLQFIGPDAYKVNIYTFHAF
                     CNDVIQENLSYFEKTVLDPISELEKIQLYKELIDSFPKNHPLKRYRGDVYFEVNNLQH
                     LFSNMKREGWTPAFIYQRIDEYIADLPNRDEFIYKRKYKEFNAGDLKKDKIEEEKDRM
                     EKLKAAVGEFDRFQLLMRKRNRYDFDDMINWVIRAFEENKNLLANYQEKFQYILVDEY
                     QDTSGTQNRLVELLISYWDKPNVFVVGDDDQSIYRFQGANVENMMQFADTYQKDLLTV
                     VLTNNYRSTQPILDISKSLIDRNNERLVNQIDGLSKQLLSSKEKIKHLTHQPVIKEYE
                     SQRQEMIGIVKQVQQLAAQGILPGSIGIIYKENKYGEELAKYFKLLNIPVYSKRHLNI
                     LELPVARKIILLLKYLASEHDIPYSGDEMLFEILHFDWFGIPPIEIAKLTMEVAGIRY
                     NENKTSIRQLLQEKANTPAKDLFSSNLHEGLKKASFVIEKLVCDVPNVTLQHLFENII
                     REAGVLTHIMQSEEKHWQLQILTGLFDFVKDETHRNHFLNLQQLVSLVELMEKEEITL
                     PLVQVSGSDKGINLMTAHGSKGLEFEYVFIAGCNAAFWEKKRKPGGGYKLPETMFTSQ
                     TAGKDDEELRRLFYVALTRAEQHLYISYSKFKNDGKELEPSMFIAEIQDQHPLPVEKI
                     ILDTEVLSEFAVLNFNEAEAPEIEKIEEEFISRILDKFVMNVTALNNYLKCPLEFYFK
                     NLIRIPSPKNEATEFGSAVHHALEKLFRKMQDGKKDQFPAKEEFIADFEWYMLRHRES
                     FTKEQFDRRLEYGLEVLNNYYDKYINSFNKIVAIERTIRNVVIKDVPLKGKLDKLEFD
                     GKSVNVVDYKTGDPDKAIPKTKGPSEKEPNGGDYWRQAVFYKILVDNYEQKDWKVVST
                     EFDFIEPDKKKNYRKEKVVIGPADITTVTQQITSTWEKIQNREFYTGCGKDDCHWCNF
                     VKTNKLAVTLHELESSDETE"
     gene            10379..11812
                     /locus_tag="PLO70_12125"
     CDS             10379..11812
                     /locus_tag="PLO70_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQZ75259.1"
                     /translation="MKTKILISYTALISILLISTISGPGCANIIPPQGGPRDTIPPVL
                     LKSNPGDSSVNFSGNKITFFFDEFVEVQNAQENLLVSPLPKITPFVDSKLKEVTVKLK
                     DTLEPNTTYTLDFGNAIKDYTEGNVFKNFTYTFSTGKHIDSFELRGNVVLAETGKTDS
                     TLIAILHTNPDDSAVVKDKPKYIARLDGKGNFYFKNLPEKTFYLYALKDEGGTRRYFG
                     EKQLFAFADKPVSLQQKNEPVTLYAFAARQETQSTTTMLPSISPGIRNKPGAATADKR
                     LKYQTTLQNNQQDLLSQFEMIFEQPLKNLDSSGIRLYTDTTYQPATDYKFVKDSTNKK
                     LTLIYEWKENTLYHLIMDKDLAEDSAGKKLLKTDTLSFNTKKKADYGALKIRFRNLDI
                     TRNPVLLFILNQNIYKSFPMTGPDFTTSLFLPGEYELRMLYDENKNGVWDPGQFLGKR
                     KQPELVKPIERKITVKPNWQNEFDIAL"
     gene            11895..12512
                     /gene="recR"
                     /locus_tag="PLO70_12130"
     CDS             11895..12512
                     /gene="recR"
                     /locus_tag="PLO70_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQZ75260.1"
                     /translation="MQFSSSLLENAVNEFAKLPGIGKKTALRLVLHLLKQDTKEVGQF
                     SEVIARMRSEIKFCQRCFNVADGDICSICSNSLRKQEVICVVESIRDVIAIESTQQFN
                     GTYHILGGIISPLDGIGPDQLNIESLVTRIEKEKTEELIFALNPNIQGDTTIYYIQKK
                     LQPYNIRITTIARGIAFGGELEYADEMTLARSLQNRLPVEKYVSR"
BASE COUNT         4120 a         2469 c         2585 g         3515 t
ORIGIN      
        1 tgcagggcta taaggaaaaa ttattttcag acccagatat tatgaaaggg gaagaaaaaa
       61 ataagagtga gtttgcaaca gcatttttgc agggtatagg taaacaatca tcactaacca
      121 gtaaagggat cactactgaa acattaacaa tgatacgcac aaggtttatt ttggactggt
      181 atgaaaataa tgctgtaaaa tatccattcc gtttatttga gtatcaccgg caattattac
      241 aggaaggaat gtttaatgcg tataatcaat ggctattcgg cgcttctgaa aatttagcgg
      301 cgtatgacaa ctggacaaag gctcatgcag aagaatataa cggttttaca acctttcaga
      361 aaagcaggat attcaagatg ccgcaggggc aatattacca ggtcaattaa taactgaaaa
      421 tagctataaa aaaagccccg gaaaacgaat ccggggcttt ttttatatag cagatttcaa
      481 ctaaattgta tattcaatgc acggttaagt acctgattgc ccaccataaa taacgattgt
      541 attatgacag aagccaaaag actatttgac tgcatagaat accacctgga acgaaagcct
      601 ttagaagata tgctggccgg aaaagagggc ggccaatgga aaaaatatag caccgcggct
      661 gtaaaggaca ctgtcgataa acttagtgca ggtttattga gtttagggat cagctgtggc
      721 gatatgagtg ccgaaggaag agataaagtt gctattcttt gtaaaaaccg gccggagtgg
      781 gtaatgcttg acctggctgt acaacaaatc ggtgctgtac ttactcccat ttatccaact
      841 atcaatgtaa atgaacttga atttgtacta aatgatgccc aggttaaaac ggtatttgta
      901 aatgatgaag aacttttttt aaaagtactc agtctgaaag aaaaagtgca cagcctgaaa
      961 gatatttata cattcgaaca tgtacccaat gccaaacatt ggaaagaggt aactgcactg
     1021 gctacccccg aattaattgc acagatcaaa cccgttgcag atagaattac gtacgaggac
     1081 ctggccacca ttatttatac gtccggaact acaggtactc ccaaaggggt gatgttatca
     1141 catcggaata ttttatcaaa tgtgatggcc tctatgcctt gttttcctcc gggagatgaa
     1201 atgcgttcat taagttttct gcctttgaac catatttttg aacgaatggt aacttatctc
     1261 tatctcttcc ggggcacctc catttattat gcagaaagcc ttgataccat tggagagaac
     1321 ctgaaagaag tacaacctca tatgtttacc actgtaccaa ggttgctgga aaaagtatat
     1381 gaccgcatca tgcaaaaggg caatgaactc acgggtacca agaaaaagct attcttctgg
     1441 gcacacagtc ttgccgagaa atttgaaatc aataaagacc tgggagcttt gtataatttt
     1501 aaactggccc tggccaacaa aattattttt agtaaatgga gagaaggact gggggggaat
     1561 attaagtgta tcgttacggg cggtgctgcc tgccaggtaa gattgatccg catctttacg
     1621 gcagccagaa taaccatcat ggaaggttat ggccttacgg aaacatcgcc ggttgttgct
     1681 gttaacaggt atgaagaaag cggaagaggc tttggtactg tagggccgtt aatagaagga
     1741 gtagaagtaa agattgcaga agatggcgaa attcttacca gggggcccaa cgtcatgatg
     1801 ggttattata agcgtcctga tcttactgca gaggtaattc aggatgattg gttcagcacc
     1861 ggcgatatcg gtacaatgac agaaaataat tttcttaaaa ttactgaccg taagaaggaa
     1921 cttttcaaaa caagtggcgg taaatatgta gcaccactac ccatagaaaa taaattgaaa
     1981 gagtctccat ttattgaaca ggtaatgctg gtaggcgcag acagaaagtt tgtcagtgca
     2041 ttaatggttc cctccttccc caacctgttt gattggtgcc ggaaaaatgg cataacagaa
     2101 accagccatg aggaagtaat ccgaaatcct aaagttatag acctctataa agaactggtt
     2161 gaaagcttta ataaattctt caaccatgtg gagcaggtaa aaaagtttga attacttcct
     2221 aatgaatgga gtgtggatac cggagaaatg actcctaaac tttcactaaa acgaaaagta
     2281 gtaatagaaa aatacaggga tgcgattgaa agaatctatt cgtaacgcta tttccccttt
     2341 gcagctgaat cctttttact gcgctgcatc attttttgta caaattcatc aaacttgggc
     2401 tgttgttccg gggtaaaaag atttcttacc cgcttaaaat gattaaaagt aagcttatca
     2461 atttcagcct gcttttcatt gattcgctgg ctgtatgcag cgattgtact atcgcttacc
     2521 ccggcatctt ttattaatgc ataaaatgct gacctggcag ccctaagaga atcgtaaaat
     2581 ggcttgatcg tcttgaaatg ctcttctttt aattgcttat gctcctttat ttgctgttca
     2641 gtcatgccca gttctttcgc catcatctca gaaggatcac cttttcctcc accggtgcgt
     2701 tttccatcat tccttccttt gccattcata agaaaaatta caaggcctat attggtaata
     2761 agtaacaata caactgcaac cgaaagaatt ttattactgg agttcttcat ttatttatcc
     2821 ttatttagat catataagat tccattatcg ttcaaccggt attcagctgc aatggattga
     2881 attgtttccg tatcaaagga tgcattttct ataaaggagc tattatcatt atcaaaaaca
     2941 acaacagcat ttatcaataa aacgaccagc aaagcagcaa taacaaaaac agggcgaagt
     3001 atccatgacc gcggagaggc cggttcaagc cctttttcca tttttgcctt aagacgggta
     3061 tagaagaaat ccggagcagg aactctctga cagccatcaa ggctattcag tatttcttcc
     3121 attttattat ttttttcagt attcataaca caccattttt gacgttaaca tgccccaaaa
     3181 ccttcgtatc taatatactt agcccatttc tttttcgagt attttcttta aattttgttt
     3241 tgcccggtgc aataatgatt ccacagccgc taccgatgtc cccatcacct cactaacttc
     3301 ttgataacta agatcttcca atttatgaag ggtaaaagca attttttgat tttcaggcaa
     3361 ttggcctatt gccttaaaca acctggccgc attctctttt ctatctaaag ccacaccggg
     3421 atgattaaaa tcaggtattt ccatctctgg ctcattgcct tccccaaata atcgctgaat
     3481 gatgccaaat ctctttttac ttttccggct tcgcagcaag tccaaagcac gggtcgttgt
     3541 gatccggtag atccaggtag aaagttttga ttcagcttta aagttttgaa cagacctgaa
     3601 aacctgtata aatacttcct gtgccacatc ttcggcatct tcagcattct gcacgatgcc
     3661 aatagcagta ttgaaaaccc tgtctttata ggtatcaacc agatacttaa aagcagcttc
     3721 atctccgttg cggagacctt gtataagctc aaactcagtc aactatgacc ttttaatttt
     3781 agagcccgaa cttacgaaat aggcggttaa ttctttccta tatttgcacc ccgcagaacg
     3841 gattagttct tggtcccgta gttcaatgga tagaatagaa gtttcctaaa cttttgatac
     3901 aggttcgatt cctgtcggga ctacaaattt ttgaaataac aaaacatgaa gttttacaac
     3961 ctgcttatta aataccgtct ctggataggt attgcgctga ttggactcgg tatttatgtg
     4021 aactacgctt cttccttctg gccggcgttt cccttatacc ttgttggtgt cattttggtt
     4081 gccggacact ttttcttcgg gcctttacgc cttatacagg agtacatgga aacgggtgat
     4141 atggatggag cggaaaaagt actggcctcc attaaatacc ctaacctgtt gtacaagcct
     4201 atccgctccg tttattttac cctgaaaggc aatattgcca tgatgaagca agacttcgat
     4261 ggtgctgaaa aaatgatgaa gaaagggctg gacctgggta tgccgatgaa ggaagcggaa
     4321 ggagccagtt tactgcaaat gggaatgctt tccatgcaga aaaatgattt gaaacaggcc
     4381 gaaagctata tccggcaggc tatacgtaag gggcttccgg ataaagaaaa cgaagcagcg
     4441 gcttacctgc aaatgtgcag cctgatgatg aataagagag agttccgtgc ggcaaaggat
     4501 ttcttcagga aaacaaaagc attaaaacct actacccctc aaattgtcga tcagatcaaa
     4561 cagattgaaa aatatatttc gagaataccg ggataacata ttcgctaaat agtttatggg
     4621 ctgacaataa cttgtcagcc tttttttatt tcttttagtt aaacgagtaa cttgctttat
     4681 ataataattc ctgtatgaaa aaagtacacc ttcttactat tatcactttt ttattttctt
     4741 ccgtatacag ccaaaaagca gggcacaaga atgaaataat ttcaataatc ccccagcctg
     4801 tatctatcat ccaggaaaaa ggttctttta cactaccata caatattgta ataattactc
     4861 cgaaaaatga tgaagtaaaa agaattgcac aacacctatc cagacaaatc aaaacagtaa
     4921 ccaaccaggt aaccattaaa gaaggcacct cagccatccc aagatcaatt cttttgtctt
     4981 tgacaggcaa taaaaacatc cccaaagatg gatatcaatt aaaaattaca actgcaggta
     5041 ttacccttct tgccaatgag ccgtcaggca ttttttatgg tgtacaaaca ttgctgcagt
     5101 tattctcaaa agaaattgaa agtactgtta tacaccaaaa accggatgcc tgggtactac
     5161 caatagccac tattaaagat catccccgtt tcggctggag aggtttgatg ctggatgtat
     5221 cccgtcactt ttttaccgtg gcagaagtaa aaaattatat tgaccagatg gtgaagtata
     5281 aattcaacct cttacacctt catttaaccg atgaccaggg atggagaatt caaataaaga
     5341 gtttacccaa actgactgaa gtgggagcat ggcgggtaga aagaacaggc acctttggaa
     5401 cgctgtcaaa accaacgcct ggcgaagccg tcacttatgg tggattttat acgcatgagg
     5461 atattaagga aatagtgaaa tatgcaggag atcgttttgt caatattctg ccggagattg
     5521 atgtgcctgg tcatagcctt gcagccattg cttcttatcc tgaattgtcc tgcacaccgg
     5581 gagagtatta tgtaagtccc ggagaccggt ttatgatctg gcccggaggc ggacaacatt
     5641 tttatggttt actggataat accatttgcc cggctttgga aaaatcattt gagtttttag
     5701 ataaagtgtt tactgaagta gcacagcttt tcccttttga atatatacat atgggtggtg
     5761 atgaaacagc ccggaatttc tgggagaaaa gtgaacagat aaaagcattg atgcaaaaag
     5821 aaaacctgaa aaacctggat gaagtgcaaa gctattttgt aaaaagagtg gagaagatca
     5881 tcaacagcaa aggcaaaaag atgattggct gggatgagat actggatggc ggtttagcac
     5941 cgaacgcagc cgtgatgagc tggagaggaa tgaagggggg tattgaagcc gccaaacagg
     6001 ggcatgaagt ggtgatgagc ccgaccgatt ttgcttatat agactacatg cagggagatg
     6061 catcgataga accaccagtg tattccactt tgcgtttaaa aaaagcgtac cagtttgaac
     6121 ctgtcccgga aggagtgaac gcttcattga tcaaaggcgg ccaggccaac ctgtggacag
     6181 aacaggtgta taacatgcga catgctcaat atatgacatg gccgagagca ttagccattg
     6241 cagaagcttt atggagccca aaagataaaa ggaactggaa tgattttgcc acaagagtgg
     6301 aaactcattt tggaagactg aatgcagcac aagtgaaata tgcacccagt atctatgatc
     6361 ccatttttga aatcactaaa aaagattctg caactattat tatcaaactt agtactgaaa
     6421 tagaaggcct gtccattcat tatagttttg ataattcatt tccggataat tattacccgg
     6481 tttacagctc gccattaata gtcccgaaag atgcttccgc attgaaggtg atcacttatc
     6541 gtgacaataa accaattgga agattgatcg tgatgccgat cgctgaaatg aggaaaagag
     6601 caggaataaa ataattttgt ggctggctgt tattgagtgc tccaaacacc aaacaactct
     6661 tccacctttt tatacctgta ttcaaatatg ccttccagct tctttttcac aaaaagtgtg
     6721 ttagccattt ttccaaaaaa ccaggcagga tttttataat gaataatatc cgtcatctcc
     6781 acaccacccg gtatttcttt aaaatggtgc tgatggtgcc ataaactata cggtccgaat
     6841 cgttgctcat caataaagta ttctttatcc tttacctgtg ttatctctgt catccagtag
     6901 acaggtatac ctaaaagcgg cttaaccgtg tactcgatta tttgtccggc atacatttta
     6961 tcgccatgat gtctggaaac aattttgaat cccatatcgc cgggtgtaat ggcctgaaga
     7021 ttagcagggt tggaaaagaa accccatgcc ctttctaatg aaatagggat tttttgaaca
     7081 gttttaattg aatgtgctgc catacaaagt aataagttaa attcgtttga ataacctttt
     7141 taaaacagaa aagttcggta accagatgtc atttaacaaa gaaagattag agcgaaaatt
     7201 tgcggaagaa tacgaaaggc tgaatgaaaa gcaaaaactg gccgtgaata ccattgacgg
     7261 ccctgttatg gtgattgcag gacccggaac tggtaaaaca caaatattag ccagtcgtat
     7321 agggaaaata ttgctggaaa ctgatgcact acctgaaaat atactttgcc tgacctatac
     7381 tgatgccggt gtggtggcca tgcgtaagag gttgttacaa ttcatcgggc cggatgctta
     7441 caaagtaaat atctatacgt tccatgcttt ttgtaatgat gtgatacagg aaaatttatc
     7501 ctattttgaa aaaaccgtcc ttgacccaat ttccgaactg gaaaaaattc aattgtacaa
     7561 agaactgata gactctttcc caaaaaacca tccgctgaaa agataccggg gtgatgtgta
     7621 ttttgaagtg aacaacctgc agcatttgtt cagcaatatg aagcgggagg gatggacacc
     7681 ggcttttatc tatcaaagga ttgatgaata catagctgat ctgcccaacc gggatgaatt
     7741 tatctataaa agaaaataca aagaattcaa tgcaggtgat ctgaaaaaag acaagattga
     7801 ggaagaaaaa gaccgaatgg agaagctgaa ggctgcagta ggtgagtttg accgtttcca
     7861 gctattaatg cgcaaacgca accgttatga ctttgatgat atgatcaact gggtcatcag
     7921 ggcctttgaa gaaaataaaa atctgctggc gaattaccag gaaaaatttc aatatatatt
     7981 ggtggatgaa taccaggata ccagtggtac gcaaaaccga ttagtcgaat tattgatcag
     8041 ttactgggac aaacctaatg tttttgtagt aggtgatgat gaccagagta tctatcgttt
     8101 ccagggagcc aatgtggaaa acatgatgca attcgcagat acctatcaaa aagacctgct
     8161 gactgttgtg cttaccaata actaccgttc cacgcaacct atactggata tttccaaatc
     8221 acttattgac cgtaataatg aaagactggt aaatcaaatt gacgggttaa gtaagcaact
     8281 gctgtcctca aaagaaaaaa ttaagcatct aacacatcaa ccagttatta aagaatatga
     8341 gtcgcagcgc caggagatga ttggcattgt aaaacaagtg cagcaactgg cagcacaggg
     8401 tattttaccg ggtagtattg gtatcattta taaagagaat aaatatggtg aagagctggc
     8461 gaaatatttc aaactgttga atattccggt ctatagcaaa cgtcacctga atattttaga
     8521 attaccggta gccagaaaaa ttattttatt gctgaaatac ctggcttcag aacatgacat
     8581 tccttatagt ggagacgaga tgctttttga aatactgcat ttcgactggt ttggcattcc
     8641 cccgattgaa attgctaagc taacaatgga agttgccgga ataagatata atgaaaataa
     8701 gacttctatc cggcagctat tacaggaaaa agcaaatact ccggcaaaag atcttttcag
     8761 cagtaacctg catgaaggtt taaaaaaagc ttcttttgta attgaaaaac tggtttgtga
     8821 tgtacccaat gtcaccctgc aacatttatt tgaaaatatt attcgtgaag caggggtact
     8881 tacgcatatc atgcaaagtg aggagaaaca ctggcaatta cagatactca ccggtctgtt
     8941 tgactttgta aaggatgaaa cacatcgtaa tcatttcctg aacctgcagc agttggtgag
     9001 ccttgttgag ctaatggaaa aagaagaaat tacattacca ctggtgcagg tcagcggcag
     9061 cgataaaggc atcaatttga tgacagcaca cggttccaaa ggactggagt ttgaatatgt
     9121 attcattgcc ggttgtaatg ctgctttctg ggaaaagaaa agaaaaccag gtggaggata
     9181 taaacttcct gaaacaatgt tcacatccca gactgccggt aaggatgacg aggaattaag
     9241 acgcttattc tatgttgcac tcacaagagc agaacagcat ttatatatct catacagcaa
     9301 attcaaaaat gatggtaaag aactggaacc atctatgttt atagcagaaa tacaagatca
     9361 gcatcctctg ccggttgaaa aaataatact tgacactgaa gtactttctg aatttgctgt
     9421 actaaatttc aatgaagctg aagcaccgga aatcgaaaaa attgaagaag agttcatcag
     9481 ccgcatactg gataagtttg taatgaacgt aacagcattg aataactatt taaaatgtcc
     9541 attagaattt tattttaaga atctcatccg gatcccgtcg cctaaaaatg aagctactga
     9601 gtttggctca gcggtacacc atgcccttga aaaactattc cgaaagatgc aggatgggaa
     9661 aaaagaccag ttcccggcaa aagaagaatt cattgctgat tttgaatggt acatgctccg
     9721 ccacagagaa agttttacca aagaacaatt tgaccggagg ctggaatacg gactggaagt
     9781 attaaacaac tattatgata aatacatcaa tagtttcaat aagattgtag cgattgaaag
     9841 aaccatccgg aatgtggtga ttaaagatgt tccgctgaaa gggaaattag ataaactgga
     9901 gtttgacggt aaatcagtca atgtggtgga ttataaaacc ggtgacccgg ataaagcgat
     9961 cccaaaaaca aaagggccat cagaaaaaga gcctaatggc ggtgattact ggcggcaggc
    10021 tgttttttat aaaatactcg ttgataatta cgaacagaaa gactggaaag tggtaagcac
    10081 tgaattcgac tttattgaac cggacaaaaa gaaaaactat cgaaaagaga aagtagttat
    10141 cggccccgct gatattacaa ctgtcaccca acaaattact tctacctggg aaaaaataca
    10201 aaaccgggaa ttttataccg gctgcggcaa agacgactgc cattggtgca attttgtaaa
    10261 gaccaacaag ctggctgtaa ccctgcatga actggaaagc tccgacgaaa ccgaataact
    10321 tttattatga ttttaaaaac aaatcattac aggcaaaccc tgtaagtttg cagcttacat
    10381 gaaaacaaag attcttatat cttatactgc ccttatcagc atccttctta tcagtacaat
    10441 cagtggtccg ggctgtgcca atattattcc gccacaaggc ggaccaagag acactattcc
    10501 ccctgtatta ttgaaatcaa acccgggaga ttcatctgta aatttctccg gaaataagat
    10561 cacctttttt tttgatgaat ttgttgaagt acaaaatgcc caggaaaacc tgctggtttc
    10621 tcccttgcct aagatcaccc cttttgtaga tagtaaattg aaagaggtaa cggtaaagct
    10681 taaagacacc ctggagccta acactaccta tacgctggat tttggaaatg ccatcaaaga
    10741 ctatactgaa ggcaatgttt ttaagaattt tacctataca ttttcaacag gaaagcatat
    10801 cgactctttt gagctcaggg gcaatgtagt attggccgaa acggggaaaa cggacagtac
    10861 ccttattgca atactacata ccaacccgga tgattcagct gtggtaaaag ataagcctaa
    10921 gtacattgcc cgcctggatg gaaaaggaaa tttttatttc aaaaatcttc cggaaaaaac
    10981 attctattta tatgcattaa aagatgaagg tggtacccgt cgctattttg gcgagaaaca
    11041 attatttgct tttgctgaca aacctgttag tcttcaacaa aagaatgaac ccgttacctt
    11101 gtatgcattt gccgccaggc aggaaacaca gagcactaca acaatgcttc cgtctatttc
    11161 ccccggtata cgcaataaac cgggagctgc caccgctgac aaacggctaa agtatcaaac
    11221 tactcttcaa aataaccagc aggatctgct cagccagttt gagatgatct ttgagcagcc
    11281 acttaagaac ttagactcat cgggtatccg gctctatact gatacaacct atcagcctgc
    11341 taccgattac aaatttgtaa aagacagtac caataaaaag ctgacattga tctatgaatg
    11401 gaaagaaaat actttgtacc acctgataat ggataaagat cttgcagaag actctgccgg
    11461 taaaaaatta ttgaaaactg atacccttag tttcaacacc aagaagaaag cagattatgg
    11521 tgctttgaaa ataaggtttc gcaacctcga tatcaccagg aacccggtcc tgctttttat
    11581 actgaatcag aatatctaca aatcattccc catgaccggt cctgatttta ctaccagtct
    11641 ttttctgccc ggagaatatg agctcaggat gctgtatgat gaaaataaaa atggtgtatg
    11701 ggatccgggg cagtttttag gtaaacgtaa acaacctgaa ttggtaaagc caatcgaaag
    11761 aaaaattact gtcaaaccta actggcagaa tgagtttgat atagctttgt aaaagccttg
    11821 agcggatagc tgcttgttaa ataaatctgg ctggcagtag cgtatagctc agatcctgct
    11881 gctatctttg acctatgcaa ttctcttctt ccctcctgga aaatgcggtt aacgagtttg
    11941 ccaaactacc cggtattggt aaaaagacag ctttacgttt agtgcttcat ttattaaaac
    12001 aggatacaaa agaagttggt caattcagcg aagtgattgc caggatgaga agtgaaatca
    12061 agttctgcca gcgctgcttt aatgtggccg atggcgatat ttgttccatc tgttccaatt
    12121 cactgagaaa gcaggaagta atttgcgtgg tagaaagtat ccgtgatgta attgccatag
    12181 aaagtaccca gcaatttaat ggtacttatc acattctcgg tggcataatt tcaccattag
    12241 atggtatcgg acctgaccaa ttgaatatcg aatcccttgt aacccggatt gaaaaagaga
    12301 aaaccgagga actgatattt gccctcaatc ctaacataca gggggataca accatttatt
    12361 acatccagaa gaaattacaa ccttataaca ttcgtataac tactattgcc cgtggcattg
    12421 cctttggcgg tgaactggaa tacgccgatg aaatgaccct tgcccgcagt ctccaaaaca
    12481 ggcttcctgt tgaaaaatac gttagccgct aagctccgtc cggcttgttt tttaagcttt
    12541 taaatcataa ttttgcagtt acattcaggc ggatttgttt attgttcggc ctgcactaaa
    12601 aacagaacta ataaacggta cggaattccc tcctggtttc ccaacgttta ttgttcgcaa
    12661 aaaaaatgtt tatgaaaaca atccagaat
//
LOCUS       DAORTI010000101        12575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5008_length_12575_cov_9.148642, whole genome shotgun sequence.
ACCESSION   DAORTI010000101 DAORTI010000000
VERSION     DAORTI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_12575_cov_9.148642"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLO70_12135"
     CDS             complement(<1..183)
                     /locus_tag="PLO70_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75261.1"
                     /translation="MNRLFNLFINVLRGYIYFSRTTLSTRKGLFRKDLKIPGLAPLHQ
                     CFFYSGSHLLLAVRIEQ"
     gene            complement(239..571)
                     /locus_tag="PLO70_12140"
     CDS             complement(239..571)
                     /locus_tag="PLO70_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQZ75262.1"
                     /translation="MKFRIESPGFKPSPSLKEFATQKLKSLDRFYKDIQEIEVTLLND
                     VKAGKELVSCTLNIRIPGKDEYIKAGSYIFEDAILKAMDAAKRRLHIRKTQLQLANKK
                     KKTSRMLV"
     gene            complement(692..1252)
                     /locus_tag="PLO70_12145"
     CDS             complement(692..1252)
                     /locus_tag="PLO70_12145"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HQZ75263.1"
                     /translation="MLNIPVQSSKRGKKDKERIRTDALLRETSTNYIRLIGDADRKAR
                     IMLVVNSVFLTIGITLLTKSMDDMPFVWISATILMLSNVLTLFFSIQSVKPEFKKDHH
                     QDCDNNILHYTKSSRIPLEDYAAQLKDTMHDNDKKMEAITRDLYHYGNLLTTKYRLLN
                     IAYHLFSWGILLSVVSYLFIILIINQ"
     gene            1469..2755
                     /locus_tag="PLO70_12150"
     CDS             1469..2755
                     /locus_tag="PLO70_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HQZ75264.1"
                     /translation="MHFITALLLGSIQISYAQPTEQFIKVIVAPDHADWVYKTGEKVK
                     FNISVLQNGNTLKNVAIKYEIGPEKMDPLKKDSLVLASGTLTTDGGTMKEPGFLRCIA
                     TATINNKTYRGLGTAAFDAQQIQPAIANPADFLSYWEQAKAELATVPLDARMTLLPER
                     CTEKVNVYHVNLQNFRPGTRLYGILCVPKKEGKYPAILKVPGAGARPYAGDIANAEKG
                     LITFEIGIHGVPVNMDASVYSNLITGALAGYWNSNLDDKDRYYYKRVYLGCVRANDFI
                     VQLPQYDGINLGVTGGSQGGALSIITAALDSRVKFLACFYPALSDLTGYLKGRAGGWP
                     HLFNKANLAFNNKKDKIEAAAYYDVVNFARLVKVPGMYSWGFNDETCPPTSMYAAYNV
                     ITAPKELFLAQETGHWTYPEQTEKLTNWLVSKLKSP"
     gene            complement(2776..3231)
                     /locus_tag="PLO70_12155"
     CDS             complement(2776..3231)
                     /locus_tag="PLO70_12155"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75265.1"
                     /translation="MKKVTSFLLVALCQSNLVTAQMTTITDSSFIVSLNQQIDNLVIE
                     QNTAALDSMYASDFVFSHGSGKIEGKQGWFISVAKGNFLTRQHDSVTVELHEGIAIVR
                     GKLSVRKKNSTLISNYHLKYVRVYANRQKRWQLISHITTAEFHAPSESL"
     gene            3345..4259
                     /locus_tag="PLO70_12160"
     CDS             3345..4259
                     /locus_tag="PLO70_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ75266.1"
                     /translation="MYRLAVLSMLLWVACTAPNRIISFTAKTKPVYSIDPLPQKILLL
                     NANDIVAKKYRDSKEELFISLTDNLMNWISKRVLEKEGIPAEVIKGYTSITGNTDSIL
                     KVLLSKYNATHAIVLQSFDVYFDQTNVEVVKGSDGVKSRTASYDIVTDIGYSLYSTST
                     LLKMMTVNRKKFHSNRSVVSGLLAAGPSVVLHREDARDMSIENAQQYLNNFFSGEANR
                     QRPVFTGKGFEAVSAAITKFEYDAALTESLRFINDPDKEKAAKACYNCAVFFEQKNQP
                     DEAIKYLRQSLSLFNLQEARWMLASLAE"
     gene            complement(4327..4695)
                     /locus_tag="PLO70_12165"
     CDS             complement(4327..4695)
                     /locus_tag="PLO70_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HQZ75267.1"
                     /translation="MWYQFFNIFFFVFHTVFTLFNIIGWAFPKTRKLHLITMLLTAFS
                     WFVLGIWYGWGYCFCTDWHWQVREILGYHDRSNSYIHFLLLKLTGKDFNPQLVENATL
                     IIFLLSFILTVWFNIKDRKK"
     gene            complement(4851..5948)
                     /locus_tag="PLO70_12170"
     CDS             complement(4851..5948)
                     /locus_tag="PLO70_12170"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75268.1"
                     /translation="MNPIVTPEILNVMKAVHYRPAVSIIMPLEQKMNLKAGLAHSLKF
                     AADKVEKELLENYPGELSILVMQKLRAMIKGLEPEVHKKSIALYVSPVFEKVLYLDMA
                     VEEKIIVDESFEIRDLIYCKKQLHKYLVLLLSGKEYRMYLGNSDSFVRIVPDAPATMA
                     VYQHDSPERVANFSDMSERKEIVMDKFLHHIDSALDTILNSYQLPLFVLGTERILGHF
                     KKLTKHEGAISKYIHGNYEEATFPQLKEMLEPHFAELKLKKQIAILHRLEESAGKKAL
                     AVGITEVWREAMNHKGQLLVVEKNYMVAAQHGSQEDVIYKVAEPATNFSYIKDAVDEV
                     IEIVLENGGDVEFIDENFLGEYQQIALVKYY"
     gene            complement(6159..6908)
                     /locus_tag="PLO70_12175"
     CDS             complement(6159..6908)
                     /locus_tag="PLO70_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQZ75269.1"
                     /translation="MDNTKDLRFAVLIDADNIPYRLIGGILEEIAKYGVPTFKRIYAD
                     WTKPHVAGWKTVLLDHAITPVQQYSYTTGKNSSDSALIIDAMDILYTGRVDGFCVVSS
                     DSDFTRLATRLREAGMKVFGMGEQKTPPAFRAACDKFIYIEILEQPETSTKQIKLTDL
                     RKKEKIISKADKALINLITSSINDVADENGWAFLGELGNLLLKKRPDFDPRNYGFEKL
                     LPLIRSLGHVVEVDERESGRKNVKLVYIKVK"
     gene            7076..7723
                     /locus_tag="PLO70_12180"
     CDS             7076..7723
                     /locus_tag="PLO70_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75270.1"
                     /translation="MKKRLFIPMRFAMLCLVLAACEKAPEVFSVNNFSMVQDSARTRM
                     GVTALTLPDITGYWTGLEHNIDVNITNGMNGITFYSVRLIDTVHSRDSGSTWFDVDFM
                     AAAGKPYAEVISWGCDRDPHEFNLVLSTYLKINKLTSDTIIVQMLNSAYTEKWLKARR
                     YNYFVTEEEKKQKEHFIYLTEDLPQLTAILKELYRVPQVFQSPDTIVRRVLTTPD"
     gene            7760..7915
                     /locus_tag="PLO70_12185"
     CDS             7760..7915
                     /locus_tag="PLO70_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75271.1"
                     /translation="MKTKRTTHRSSSGKKLYAVRDANGRFVDIQTFERAHRDDLRKKH
                     TKSDRKK"
     gene            complement(8067..9455)
                     /gene="nhaD"
                     /locus_tag="PLO70_12190"
     CDS             complement(8067..9455)
                     /gene="nhaD"
                     /locus_tag="PLO70_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQZ75272.1"
                     /translation="MIIFPEGFLFLHFAATWLTTLINRRHLKNNNQPANMIIAIAITF
                     VLGYFTIAMEGPLKVNKAAAALVTAGLCWSLFAVLSGDSNRVAEKLTSQLGSVSGILF
                     FLLGAMTIVELIDANDGFYLITKRIKTTDKKKLVWLVAFITFFLSSILDNLTTTIVLV
                     SLLRKIISDKQERLMFAALVVIAANAGGAFSPIGDVTTTMLWIGGQITTYSIILQTFL
                     PSLISLLVPALLISRQMKGRLQVREDSAVLNENFNTNAFDRNLVFGLGISCLLFVPVF
                     KTLTGLPPFLGMLFGLGLLWVITELKDSKRDEEEKGPLSVNHALRKIDTASVLFFLGI
                     LLTIGALEATGILDNVATVMNEKIGHITIITLVIGMLSAVVDNVPLVAAAQGMYPLSV
                     YPTDHYFWEFLAYCAGTGGSMLIIGSAAGVAAMGLEKIDFIWYLKKVSWLAIIGYFAG
                     AAVFLLQHYLMQ"
     gene            9557..10627
                     /locus_tag="PLO70_12195"
     CDS             9557..10627
                     /locus_tag="PLO70_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQZ75273.1"
                     /translation="MVVLFMMQSNSFLLYGANGYSGELIARYAKQYELEPILAGRRKE
                     IIAPLAGKFGLPYRVFDVDDTTALLAALREVKVVMNAAGPFQFTARQMIEACLQTGTH
                     YLDINGDIAVFEMIKRYDEAAKKAVIMLLPGAGFDVVPTDCLSLHLKKLLPDAVSLQL
                     AFASVGGGLSHGTATTMANKIGEGGAARKNGKIIRQSLGKKGMWIDFGIKKLFVMSIP
                     WGDISTAYFTTGIPDIESYTGMSRKVYYILKVQPLFNWLLRTNFVRNGLKKKINSRPA
                     GPSDEQRNKSISLVWGQVSNATGEIVTARLSGPEVYTLTAYSALLIIQKVLTGNFSAG
                     YQTPASAYGENLILEVPGVQWR"
     gene            complement(10830..11270)
                     /locus_tag="PLO70_12200"
     CDS             complement(10830..11270)
                     /locus_tag="PLO70_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQZ75274.1"
                     /translation="MKKIIPFILVTTSVIVVLAACTRNPYAVQPAVTTDTTGFAQFRE
                     WKTMNELESAIALRTAVAPRKTNTVYKSGSMTSSTTNEAKVAKKKGWSKAAKYSVIGA
                     GSGAILGAVINKRNRVAGGIIGGVLGGGVGYGIGRSNDKKDGRL"
     gene            complement(11542..>12575)
                     /locus_tag="PLO70_12205"
     CDS             complement(11542..>12575)
                     /locus_tag="PLO70_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HQZ75275.1"
                     /translation="AQEKHPYNVIAYYTGKGSTIQQYPVHTLTHIIYSFLKLQQDTLT
                     FHNDEQRQTVQQLVQLKKQYPQLKIMVSIGGWGGCAHCSELFRSATHRETFAKTTVAL
                     FKEYAIDGLDLDWEYPAIEGYPGHTYALHDKENFTALVKKLREEMGNRYLLSFAAGGF
                     TGYIDQSVDWKAVMPQVDFVNLMTYDLVGGYSTVTGHHTPLADYRPGQQSVQKCVNQL
                     LQKNIPADKLIIGAAFYARVWEQVADSNHGLYQTGKFKQGVSYKMFPQYFSDSSGFNY
                     YWDKKAKAPYQYNRRKKLFATFDDKNSIREKTKFIRRKKLGGIMFWELSDDLPVNGLL
                     DEIHKTLNN"
BASE COUNT         3667 a         2712 c         2607 g         3589 t
ORIGIN      
        1 ctgctcaatc ctgacagcca gcaaaagatg ggagccgcta taaaaaaagc actggtggag
       61 tggagcaagg ccaggaatct ttaaatcttt ccggaacaaa ccttttcttg tggataaggt
      121 ggtcctggaa aaataaatat acccccgcag gacattaatg aacaaattga ataaccgatt
      181 cattaaaaat cctgttttca caaatccgga actttgcctg ccgggtactc cggtggactt
      241 ataccagcat acggctggtt tttttctttt tattagccag ctgcaattga gttttccgga
      301 tatgtagtct tcttttggca gcatccatcg ctttcaatat ggcatcttca aaaatataag
      361 aaccggcctt gatgtactca tcctttccgg gaatacggat attcagtgta caactcacca
      421 gttccttacc agctttcaca tcgttcagca gtgtcacttc aatttcctgg atatctttat
      481 aaaacctgtc caatgatttt agtttttgtg ttgcaaactc tttcaatgaa gggcttggtt
      541 taaaccccgg tgactcaata cggaatttca tgtttttaag ttgaagggta ttaacaataa
      601 ttatcaacag tatcaggggt ctgcctctat cggtattaaa gaccccgttg tgatctctcc
      661 atcgatatat cagcgttaca ttttttagag tttattggtt aatgatcagt attataaaaa
      721 gatatgaaac tacagacagt aaaatgcccc atgaaaaaag gtggtaagct atattcagga
      781 gcctgtattt cgttgtgagg agattgccat agtgatacag gtccctggtg atggcttcca
      841 tttttttatc attgtcgtgc atggtgtctt tgagttgtgc agcatagtct tccagcggaa
      901 tgcggctgct tttcgtataa tgcaggatat tgttatcgca atcttgatga tggtcctttt
      961 taaactctgg ttttaccgat tgaattgaga aaaaaagcgt cagcacattc gacaacatca
     1021 gaatagtggc actgatccat acaaagggca tgtcatccat ggatttggtt aacagggtaa
     1081 tgccgatagt caggaaaaca gaattcacaa ccagcatgat cctggcttta cgatcggcat
     1141 cacctatcaa tcgtatatag ttggtagaag tttcccggag cagcgcatca gtcctgattc
     1201 gctccttatc cttcttccct cttttcgacg attgtacagg tatgttcagc atgatcagtt
     1261 ccccattttc tttccacttg taaattaata ctagttgcgg cccgcttcaa tgagtaaact
     1321 cattcaaaac cctgataaga attactaacc cgggaagcag gcaaagctgt tgcaggcaaa
     1381 gtctgccagg aagcccggtt tacaattttg ttttcgtaaa ttccctgaaa ataactgtat
     1441 gcccactgcc acttgttata aaaaaattat gcattttatt actgccctgt tattgggaag
     1501 tattcaaatc agctatgccc aacccactga acaattcata aaagtaatag tggcaccgga
     1561 tcatgctgat tgggtttata aaaccggtga aaaagtaaaa ttcaatatct ctgttttaca
     1621 aaatggcaat acgcttaaaa atgtagctat caaatatgag attggtccgg aaaaaatgga
     1681 tcccctgaaa aaagactctt tagtgcttgc cagcggaaca ttgacaacag atggaggtac
     1741 catgaaagag cccggttttc ttcgctgcat tgctaccgca acgatcaata ataaaaccta
     1801 tcggggatta ggtacggctg cttttgatgc acagcagata caacctgcca tcgctaaccc
     1861 ggcagatttt ttatcctatt gggaacaggc aaaggctgag cttgcaactg tgccactgga
     1921 tgcgagaatg accttactgc ctgaacgctg tacggagaaa gtaaatgtat accatgtcaa
     1981 cttgcagaat ttcagaccgg gtacccggtt gtatggcatc ttatgtgttc ccaaaaaaga
     2041 aggtaaatac ccggcaatac tcaaagtgcc gggtgctggt gcccgtcctt atgcgggaga
     2101 tatcgccaat gcagaaaaag ggcttattac ttttgagatc ggcattcatg gtgtaccggt
     2161 gaatatggat gcttccgttt acagcaatct tattaccggt gcgttggcag gatactggaa
     2221 cagtaatctt gatgataagg accgctatta ttacaaaagg gtgtacctcg gctgtgttcg
     2281 tgccaatgac tttattgtac aactgccaca gtatgatggg atcaatctcg gcgttacagg
     2341 aggcagccag gggggcgcct tatcaatcat taccgcagcg ctggattcaa gggttaagtt
     2401 tcttgcttgt ttttacccgg ccctctctga tctgaccggc tatttaaaag gccgtgccgg
     2461 cggctggcct catttattta ataaggccaa tcttgctttt aataataaaa aggataagat
     2521 agaagctgct gcttattatg atgtggtcaa ttttgcccgc ctggtaaaag taccgggaat
     2581 gtatagctgg ggcttcaatg atgaaacctg tccgcccaca tcaatgtacg ccgcctataa
     2641 tgtgataaca gctcctaaag aattattcct tgcacaggaa acaggacact ggacataccc
     2701 ggagcaaaca gaaaaactaa caaactggtt ggtctcaaaa ttaaaatcac cttaacagct
     2761 gcaatgagtt tgttattata aactttctga tggcgcatga aattcggcag tagtgatatg
     2821 tgatattaat tgccaccgct tctgacggtt ggcataaacc cggacatatt tcaaatggta
     2881 attggagatc aacgtactgt tcttttttcg taccgataat ttacctctta caattgcaat
     2941 tccttcatgc agttctacgg ttacagaatc atgctgacgg gtcaggaaat ttcctttcgc
     3001 cactgatata aaccaaccct gtttaccttc aattttgcca gagccatgac tgaaaacaaa
     3061 gtcactggca tacattgaat ccaatgccgc tgtattttgt tctatgacca ggttatcaat
     3121 ttgctgattg agtgaaacaa taaaactgct gtcagtaatc gtagtcattt gtgccgttac
     3181 caggttcgac tggcaaagag ctaccagtaa aaaagaagtt acttttttca tgcaataaag
     3241 ttagctgatt actacggtta ccggcctgat agtataacga aaagactgag tatagtcatt
     3301 tgaaacagat gtttggtact gtaacttgct gcaaattgaa ttttatgtac cggcttgcag
     3361 tgctatctat gctattgtgg gttgcctgta cggcccccaa ccggatcatt tcatttacgg
     3421 caaaaacaaa acctgtttat tccattgatc cgctgccgca aaaaatactc ctgctcaacg
     3481 ccaatgatat cgttgctaaa aaataccggg atagcaaaga ggagctgttt atttccttaa
     3541 cggataacct gatgaactgg atatcaaaaa gggtactgga gaaagaaggt atcccggccg
     3601 aggtaataaa gggttataca tcaatcacag gaaataccga cagcatcctt aaagtactgt
     3661 tgtcaaaata taatgccacc catgctatag tactacaatc ttttgacgtt tatttcgacc
     3721 agaccaatgt ggaagtagtg aaaggaagtg atggtgtgaa aagcaggaca gcctcatatg
     3781 acattgtcac agatatcggc tattcacttt attctaccag taccctgctt aaaatgatga
     3841 ccgttaaccg aaaaaaattt cactccaaca ggtcagtggt gagtggtttg ctggcagcag
     3901 gtccgagtgt ggtcctgcac cgggaggatg cacgggatat gagtatcgag aatgcacaac
     3961 aatacctgaa caattttttc tccggtgaag ccaatcgtca acgtccggta tttaccggca
     4021 aaggatttga agcagtgagt gctgctatta caaaatttga gtatgatgct gcccttacag
     4081 aaagcctgcg attcatcaat gatcctgata aagaaaaagc agccaaagcc tgttataatt
     4141 gtgcagtgtt ttttgagcaa aagaatcagc cggatgaagc aataaaatac ctgcgacaat
     4201 cgctttccct ttttaacctg caggaagcaa ggtggatgct ggcttcttta gcagaataag
     4261 actattccct gttacatcca caggacaccc attcaagttt tttaaaccgg tttatccttt
     4321 taatcattac ttcttccggt cctttatatt aaaccatact gttaatataa aactcagcag
     4381 gaatataatc agtgttgcat tctccaccag ttggggattg aaatctttac ccgttaattt
     4441 caacagaaga aaatgaatat aagaattcga ccggtcgtga taacccagta tttctctgac
     4501 ctgccagtgc cagtcggtgc aaaaacaata gccccagcca taccagatgc cgagtacaaa
     4561 ccaggaaaaa gcagtgagca gcatcgtgat caaatgcaat tttcttgttt ttggaaatgc
     4621 ccagccaata atattgaata acgtaaatac tgtgtgaaag acaaagaaaa agatattgaa
     4681 aaactggtac cacatgctga gagccgatgt gctgtaaaga taaaataacc ccggtagcta
     4741 tgcacttgtt ttattgatcc caataactat gaaaataaaa atatttaatt acgatggggc
     4801 ccacgcaggg aacccgtcat agaaaggaag tgaatttgct tcaaaaaata ttaataatat
     4861 ttcaccaatg ctatttgctg gtattctccc aggaaatttt catctataaa ttccacatca
     4921 ccgccatttt ccagtacaat ttctataacc tcatccactg catcttttat gtaagaaaaa
     4981 ttagtagctg gctccgccac tttataaatc acatcttcct ggcttccgtg ttgtgctgcc
     5041 accatataat ttttttccac caccagcaac tggcccttat gattcatcgc ttcacgccat
     5101 acttcggtaa taccaacagc cagagctttt ttcccggcag actcttccag ccggtgcagt
     5161 atcgctattt gtttttttaa tttcagctct gcaaaatgag gttccagcat ttccttcagt
     5221 tgcggaaatg ttgcttcttc ataattaccg tgtatatatt tactaatggc tccctcatgt
     5281 ttggttaatt tcttaaaatg ccccagtatt ctttcagttc ccagtacgaa tagcggcaat
     5341 tgatacgaat tgagaatggt atccagcgcc gaatcaatat ggtgtaaaaa tttatccatt
     5401 actatttctt tccgctccga catatcagaa aagttcgcta ctctttccgg cgaatcatgc
     5461 tggtagacag ccatcgtggc aggtgcatct ggtacaatcc ttacaaaact gtccgaatta
     5521 ccaaggtaca tgcggtattc ttttccgctc agcaacagca caaggtattt gtgcaattgt
     5581 tttttgcagt atataagatc ccggatctca aatgattcat ctacgataat cttttcttcc
     5641 accgccatgt cgaggtagag tactttttca aacaccggcg atacatataa ggcaatactt
     5701 tttttatgta cttccggttc caggccttta atcatagccc gcagcttctg catcaccagt
     5761 atagacaatt ccccgggata attttccagc agctcctttt ccactttatc ggcagcaaac
     5821 ttcagcgaat gcgccagccc ggctttcagg ttcatttttt gttcaagcgg cataatgata
     5881 gatacagcag gccggtaatg caccgctttc attacattca gtatttccgg agtcacaatt
     5941 gggttcataa caaactattt aagtttgaaa tctactcctt tatatctcca aaaacagtga
     6001 caggtatcac ctgtagcttt gattattctc attacaaatg gaatgttccg ggttatgtta
     6061 ctggtgagga tgcggaggca taaattccgg aacggaacta ttgaaggtcc ttaatgttac
     6121 ccggaatccc gggctccctt tacgggcaag gagaaagatt attttacttt gatatatacc
     6181 agctttacat ttttccttcc tgattccctt tcgtctacct ccacaacatg gccgagactg
     6241 cggataagcg gcaataattt ttcaaatcca taattacggg gatcaaaatc aggacgtttt
     6301 ttcaataaca aatttccgag ctcacccagg aaagcccacc cgttctcatc tgccacatca
     6361 ttgatgctgg aggtgatcag gttaataagg gccttgtcag ctttactgat aattttttcc
     6421 ttcttccgca gatctgtcag tttgatctgt ttagtgcttg tttcaggctg ttccaatatt
     6481 tctatataaa taaatttatc gcaggcagcc cggaaggcag gaggagtttt ttgttccccc
     6541 ataccaaata ctttcatgcc ggcttcccgc agccgggtcg ccagccgggt aaaatcacta
     6601 tcactgctga ccacacaaaa tccatccacc cggccggtgt ataatatatc catcgcatca
     6661 atgatcagtg ccgagtcaga agaattttta ccggtggtgt aactgtattg ctgtacaggg
     6721 gtgatggcat gatccagcaa aacggttttc cagcctgcaa catgtggttt tgtccagtcg
     6781 gcataaattc gtttgaatgt aggcactcca tatttggcaa tctcttcgag tataccaccg
     6841 atcaggcgat acggaatatt atcggcatct attaataccg caaagcggag atcttttgtg
     6901 ttgtccatgt ataaaaaatg attggtgcag gaaaggtaat actaaaaaga atccgcctcc
     6961 tgtatccgtt tcccgggatt gatcactttt tcattgatca ttatcagtcc tgccggtacg
     7021 gccagtcata ataaacagcc ttttgactgc tgtaacttaa cagggagttt agtgtatgaa
     7081 aaagagattg ttcataccca tgcggttcgc tatgctttgt ttggtactgg cagcctgcga
     7141 aaaggcaccg gaagtttttt cggtcaataa tttttctatg gtgcaggaca gtgcccggac
     7201 ccgcatgggg gtgacagcat tgactctccc tgatataacc ggctactgga ccgggctgga
     7261 acataatata gacgttaaca ttactaacgg aatgaacggg attacctttt attccgtcag
     7321 gctgattgat acggtgcatt ccagggatag cggcagcacc tggtttgatg tggattttat
     7381 ggcagctgca ggtaagccat atgcagaagt gatcagttgg ggatgtgaca gggatcctca
     7441 tgaattcaac ctggtattaa gtacctattt aaagatcaat aaattgacca gcgatacgat
     7501 catcgttcaa atgctcaata gtgcatatac agaaaaatgg ctgaaggcaa ggcggtataa
     7561 ctattttgtt accgaagaag aaaaaaagca gaaagaacat tttatttatt taactgaaga
     7621 cctgccgcag ctcactgcta tattgaaaga actataccgg gtgccacagg ttttccagtc
     7681 acctgataca attgtgagaa gggtgctaac aacgccggat taaaacctta ttaatcgttt
     7741 ataatttaaa attgatgtta tgaaaacaaa aagaaccact caccgcagca gcagtggtaa
     7801 aaaattgtat gcggtaagag atgccaatgg ccggtttgta gatatacaaa cctttgaacg
     7861 ggcccatcgg gatgacctga gaaagaagca taccaaaagt gacaggaaga aataatttgt
     7921 tcccgcaact actttaagtt gattgcccgg gatattgaag gacgcaatat atacgcactg
     7981 attcattctg gttgctgccg gaagagaagg ggtgttgtta ttttttacat aacaatagcg
     8041 gatcacctgt taatcttagc acagcatcat tgcattaaat aatgctggag cagaaataca
     8101 gcagcaccgg caaaatagcc gataatagcc agccagctaa cttttttcag gtaccatata
     8161 aaatcaattt tttcaagtcc catagcagct accccggcag ctgacccaat gatcagcata
     8221 ctaccaccgg taccggcaca ataggcgagg aactcccaaa aataatgatc ggtaggatag
     8281 accgacaaag gatacatgcc ctgcgcagct gccaccaacg gtacattgtc cacaactgca
     8341 gacaacatac ctataactaa agtgatgatc gtaatatgtc caatcttttc attcatcaca
     8401 gtggctacat tatctaaaat accggtagct tccaaagccc cgatcgttaa caggatacca
     8461 agaaaaaaaa gtacactggc cgtgtcgatc ttccttaatg catgattgac agatagcgga
     8521 cctttttctt cttcatctct tttactgtct tttaattcgg tgatcaccca caacaggccc
     8581 agcccgaaca gcatgcccag gaaaggaggc aggccggtaa gtgttttaaa taccggcaca
     8641 aataataaac aggaaatacc taatccaaaa acaaggttcc ggtcaaatgc gttggtatta
     8701 aaattttcat tgagcactgc actatcttct cttacctgta aacggccttt catctgccgg
     8761 ctgatcagca gtgccggcac taataaactt ataaggctgg gtaaaaaggt ttgcaggatg
     8821 atggagtatg tagtgatctg tcccccgatc cataacatgg tagtggtaac atctccaatg
     8881 ggagagaagg ccccgccggc attggcagct attaccacca gtgcggcaaa catgagccgc
     8941 tcctgtttat cggatatgat tttccggagc agcgatacca atacaatggt agtagtgaga
     9001 ttgtccagta tggacgataa aaaaaaagtg atgaacgcaa ccagccacac taacttcttt
     9061 ttatccgttg ttttgatccg tttggtgatc aggtaaaacc catcattggc atcaatcagt
     9121 tcaacgatcg tcatggctcc gagaagaaaa aataaaatac cggaaactga acccagttga
     9181 ctggtaagtt tttcagccac cctattactg tcaccggaca aaacagcaaa cagtgaccag
     9241 caaagacccg ctgttaccag ggctgccgct gctttgttca ctttcaacgg accttccatg
     9301 gcgatagtga agtagcctaa tacaaatgtg atagcaatgg caatgatcat attagccggt
     9361 tgattgttgt ttttaagatg ccgcctgttg atcagtgttg tcaaccaggt cgctgcaaaa
     9421 tgaaggaaca gaaaaccttc aggaaagata atcattaatg aacggctatc cggttgacat
     9481 ttttcagaaa tagccggctt tgttataaga gggtcttgca gcgtgatggc aaaaacaagg
     9541 ctggtaatac cattctttgg ttgtactttt catgatgcaa tccaactctt tcctcctgta
     9601 tggcgccaat ggttatagcg gtgaattaat tgcccgttat gcaaaacaat atgaactgga
     9661 acctatttta gcaggccgga gaaaagaaat aatagcaccg ctcgccggta agttcgggct
     9721 cccctaccgg gtttttgatg tggatgacac aacggcttta ctggccgcct tacgggaagt
     9781 aaaagtcgtg atgaatgctg caggaccttt ccagtttact gccagacaaa tgatcgaagc
     9841 ttgtttacaa accggcacgc attacctgga catcaatggt gatattgcgg tatttgaaat
     9901 gataaaacgt tatgatgagg cggctaaaaa agccgttatc atgctgctgc ctggtgcagg
     9961 ctttgatgtg gtgcctaccg attgcctgtc actccattta aaaaaattat tacctgatgc
    10021 agtctccctg caattagctt ttgccagtgt aggcggcgga ctctctcatg gtactgccac
    10081 tacaatggcg aataaaattg gcgaaggagg tgccgccagg aaaaacggga agatcatccg
    10141 gcaatcactc gggaaaaaag gaatgtggat agattttggt attaaaaaat tatttgtgat
    10201 gagcattccc tggggtgata tttccaccgc ctattttaca acgggcatac cagatattga
    10261 aagctatact ggcatgtcaa gaaaagtata ttatatatta aaagtgcaac cgctttttaa
    10321 ttggctgttg cgaacaaatt ttgtccgcaa cgggctgaag aaaaaaatca atagcaggcc
    10381 agccggtcca tctgatgaac agcgaaacaa atctatcagc ctggtatggg ggcaggtaag
    10441 taatgctaca ggtgaaatag ttacggcacg gctcagcggc cccgaagtat atacactgac
    10501 cgcatacagt gcattactaa taattcaaaa agtattgaca ggaaattttt ctgctggtta
    10561 ccagacacct gccagtgctt atggtgaaaa tttaatactg gaagtgccgg gagtacaatg
    10621 gagatgatga ttaaagaata aacaggaagt acaatcgcat acgttaaaaa acaggggccg
    10681 tttctaaagc aatcaaatct gttacattgt cccggtcgct accgggctgc cggattattt
    10741 aaagatttgt ttttcagcga ttgtcatatt tcgatatgcc caatatgtca atcgctaaaa
    10801 cattagaaac ggcctctgcc tgattcaatt tataatcgtc cgtctttctt atcattggaa
    10861 cggccgatac cataaccaac accaccacct aatactccgc caatgattcc accggctaca
    10921 cgattgcgtt tattgatgac ggctcccagg atcgctcctg aaccagcacc gatcactgaa
    10981 tacttagcag ctttactcca acctttcttt ttagcaactt ttgcttcatt agtagtggag
    11041 gaagtcattg aaccactttt ataaacagta ttggttttcc gcggtgcaac agcggtacgc
    11101 agggcgatag cagattccag ctcattcatg gttttccatt cacggaactg tgcaaagccg
    11161 gtagtgtccg tagttactgc cggctgaacc gcataaggat tacgggtaca agctgccaat
    11221 actacaatca ctgatgttgt tacaagaata aagggtataa tttttttcat aactgtcaat
    11281 ttaaaaatga ataaataaaa taacagctgc agtaattcac aaacggtgcc agagatcatt
    11341 gacttgttaa cagaatcctt tacaggatgt caactggcta agcagaaggt ctttagaaga
    11401 actacctttt aacagtgctg attataataa ctgatgatga ataattaact cttgttttta
    11461 acaggaataa ttacagaaac attctgaggc ggattgggtt accttcttct atggatatac
    11521 aatgcagata aaaataaaat atcagttatt cagtgttttg tgtatctcat ccagcaaccc
    11581 gtttaccggc aggtcatcgc tcagttccca gaacatgata ccacccaatt ttttccttcg
    11641 gataaatttt gttttttccc ggatagaatt tttatcatca aaggtggcaa ataatttttt
    11701 gcgccggttg tattgatacg gtgcttttgc ttttttatcc caatagtagt tgaacccgga
    11761 actgtcgctg aaatattgcg ggaacatttt ataggatact ccctgcttaa attttcctgt
    11821 ttgatataaa ccatgattac tgtctgccac ctgctcccac accctggcat aaaaggcggc
    11881 cccgatgatc agtttgtctg ctggaatatt cttttgtaaa agctggttca cacatttttg
    11941 aacagactgc tgcccgggcc tataatcagc cagcggtgta tggtgaccgg ttacagtact
    12001 gtaacctccc accaggtcat aggtcatcag gttcacaaaa tctacttgcg gcattactgc
    12061 cttccagtca acagactgat cgatataacc ggtaaaccca ccggcagcaa aactgagcaa
    12121 atacctgttg cccatttctt ctcttaattt ttttaccagt gctgtaaaat tctctttatc
    12181 atgcaaagca taggtatgac cgggatatcc ttctatggcc gggtattccc aatccaggtc
    12241 gaggccatca atagcatact ctttaaataa agccactgtt gttttcgcaa atgtttcccg
    12301 gtgtgtggcc gaacggaata attctgagca gtgtgcacag ccaccccagc caccaatgga
    12361 caccatgatc tttaactgcg ggtattgctt ttttaattgt acgagttgct gtaccgtttg
    12421 ccgctgctca tcattatgaa aagtgagggt atcctgctgc agcttcaaaa aactataaat
    12481 gatatgagtg agggtatgaa cggggtattg ctgaatagtg cttcccttac cggtgtaata
    12541 agcaattaca ttatacgggt gtttttcctg tgcaa
//
LOCUS       DAORTI010000102        12499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5055_length_12499_cov_6.692221, whole genome shotgun sequence.
ACCESSION   DAORTI010000102 DAORTI010000000
VERSION     DAORTI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_12499_cov_6.692221"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1566
                     /locus_tag="PLO70_12210"
     CDS             79..1566
                     /locus_tag="PLO70_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75276.1"
                     /translation="MKTKALHILRTRPIFLYLLPVFFVLHGYTENYDFVPVNDAVILL
                     ATYLIASLILSLLAWIFYRNFTKANLLAFCMMAFHFFFGAVQDLIKKISPDIFILKYS
                     FILPCTAILFIVLLISLKKTKKSFLKITLFTNLLLLLFILADSGWLLLKVLTVKEIKR
                     APVSGLFKKCDNCKYPDVYLIMADGYPGQTELKAVLGYDNQLFEQELEKRGFHVIDSS
                     RSNYNLTPFSVASMLDMDYLKGIAGQYSNKNDIALCYNTIKQSKLFSFLKQTGYKIYN
                     YSIFDLTNQPSLATPTFLPRKSKPITAQTFISRAEKEIGFHLATTLKIKSIIQYVRNH
                     DLKNNRKLTAKTIKIAKEATSNPKFVYTHLIMPHYPYYFDSTGQAVSYEKITDDFIVD
                     QGAMISYLKYSNTKLIELVDQIKSSAASPPIIILMSDHGFREFKVPVDNKYHFYNLNA
                     VFFPDSNYSLFYNGMTNINQFRVILNSQFGQQLPLLKDSTSFLLE"
     gene            1603..2235
                     /locus_tag="PLO70_12215"
     CDS             1603..2235
                     /locus_tag="PLO70_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQZ75277.1"
                     /translation="MFDPQKILVLAPHTDDGELGCGGTIAKFCAEGREVFYAAFCLCS
                     KSLPSNLPADTLEQECKKATELLGIRPSNLVLFNYEVRELPQSRQKILEELLQLNKKI
                     SPDMVLLPAASDVHQDHQVIHQEGLRAFKNTTFAGYELPWNNYSFHTNFFMRLSETEL
                     NKKAAALKAYQSQSHRNYMQEDFIRSLAKVRGVQCNSEFAEAFEIYKLIS"
     gene            complement(2238..3299)
                     /gene="porQ"
                     /locus_tag="PLO70_12220"
     CDS             complement(2238..3299)
                     /gene="porQ"
                     /locus_tag="PLO70_12220"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HQZ75278.1"
                     /translation="MFLRFTILFLLVSGYCSAQVLGGLSVFNFLRLSNTPQLTALGGV
                     NISQQSDDIGLAFNNPALLTPRMHSQMNAVFNDFYGGIKVFHLSLGYRNEKLKTNFLW
                     GLNYFNYGSTTETDAGGNVFGKFRPTDWVMQVSASRSYLEKWNYGAALKWISSNYGQF
                     RSNGIATDIGVLFSDTTRLFSASFLVKNLGVQLKRYNNAEQEELPFDLQAGITKRLEK
                     APFSFSLTAQRLQQFDILYNDTAFNNENGYGNSSSNKFSFDKLLNHLILSTTIYIGDR
                     VEVQGGYNFIRRKELNIGNAGNGMNGFSMGAGVKLGKLSIRYARSYYQHNSAFNQLGL
                     NMKLNEYFGLGKFGERIGW"
     gene            complement(3545..5953)
                     /gene="lon"
                     /locus_tag="PLO70_12225"
     CDS             complement(3545..5953)
                     /gene="lon"
                     /locus_tag="PLO70_12225"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQZ75279.1"
                     /translation="MDIEKYMLRSEDEMDFLPIIPLNEGDQEDPNGVEIPAEIALLPL
                     RNTVLFPGVVLPITVGRDKSIKAVNDAYKTDKLIGVIAQKDSNVEDPEIKDLEKVGTV
                     AKIVKMIKMPDGGTTIIIQGKSRFQIDTVFEDDPYFKAKITKLEEEESPKDEDFNAYV
                     ANIKDLAADIVQLSPNIPTEASIILRNIENPSFLIHFVSSNLNTDIKDKQRLLELNQI
                     RQRADLLMQLLQKELQFAELKNKVTTKTRTELDKQQREYFLQQQLKSIKEELGGDSNS
                     QEIKEMQKKAEAKKWPAAAKEMFKKGVEKLERMHPSTPDFSVVYNHLDLMLDLPWEEY
                     TEDHYDLKKAKETLDHDHYGMQKIKERILEYLAVLKLKGDMKSPILCFVGPPGIGKTS
                     LGRSIANAIGRKYVRLSLGGLHDESEIRGHRKTYIGAMPGRILQSIRKIKSSNPVMIL
                     DEIDKVGNDFRGDPSSALLEVLDPEQNYTFYDNYLELEYDLSKVLFIATANNIQNIQP
                     ALRDRLEIIDLSGYAVEEKIEIAKRHLLPRQKDLHGLKNTNFRISDKMLEKIIQDYTR
                     ESGVRELDRQLASIMRYQAKELALKEKVKTVLGPDDIEQILGKPRYSNDIYKIANMAG
                     VAIGLAWTYVGGEILFIETSLSDGKGELKLTGNLGNVMKESASTALTYLQSNAKRYKL
                     DPKYFDKKNIHIHVPEGAVPKDGPSAGVTMMTAIASAMTGKKVKPFLAMTGEITLRGQ
                     VLPVGGIKEKVLAAKRAGLKEIILCSQNEKDVEEIESEFIKGIKFHYVKTMNQVLELA
                     LVSK"
     gene            complement(6141..6725)
                     /locus_tag="PLO70_12230"
     CDS             complement(6141..6725)
                     /locus_tag="PLO70_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQZ75280.1"
                     /translation="MKTKFLSLFAAVLLSQAMMAQFHLGVKAGANITKVDGKSFKDEF
                     RYGYHLGGFAEIRLGNKLVLQPEVLFNQYATRLDSNYKNVYEDVFDGNSNIKLNYLSV
                     PVLLNYKLVGNFISLQAGPQFGVLLDQNKTLLQNGGNAFKSGDLSMLAGVLVRVGPMR
                     INGRYAIGLNNISDIADDDKWKNQGFQVSVGLAL"
     gene            complement(6907..7935)
                     /locus_tag="PLO70_12235"
     CDS             complement(6907..7935)
                     /locus_tag="PLO70_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75281.1"
                     /translation="MLKKWSILLAFIIGSVAAHSQSNAGDSTIIPVAEVSLIDTSIDY
                     DELFRDFDAFLDSILTPRSYFLSSLSVGKGYYNFANKSNYTVTTSKKLNYSPTLGYYH
                     KGGLGLTAIGYIVDDGKNMNFYQSGITPSYDYLQNRKFATGISYTRYFTKDSLPFYTT
                     PLQNELYAYFLYRQSWVKPSLALSYGWGSRSDYMQREELIQDLRLRRRGFTYINTEET
                     VSDFSLMASVRHDFYWLDIFTLKDHIRFTPQLTFTSGTQKFGFNQSSATYATTIRTGS
                     NILFNSDNVYLDDQINFQPLSLSLYLRGEYSIGKFFLQPQLILDYYFPATSANFNTLF
                     SLNAGFFF"
     gene            complement(8111..9361)
                     /locus_tag="PLO70_12240"
     CDS             complement(8111..9361)
                     /locus_tag="PLO70_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ75282.1"
                     /translation="MRNSPFWWIFISFMVLLDVYFFQVLKVITHSASSRTRLLIHSGY
                     WIVSIGAILMLLLLPYLHFDKQAKLAKSTIFAMIAGLFFAKLIASLFFVVDDVRRGIQ
                     WAGTKLFFKPKEAGITPVGDGISRSVFLSWAGMLAGSGLFGTLIYGFGNKHRYQVTKI
                     KLDYPNLPQAFRGIKIAHISDIHAGSFSDKEAVIKGVQKLMLEKPDLILFTGDLVNNV
                     ASEMDEYMDVFNKLNAPLGVYSTLGNHDYGDYVEWDSPEEKNTNLERLKQVHAAMGWK
                     LLMNEHVVLEKESDSIALLGIENWSSKARFPKYGDLKKAYAGSEKHPFKILLSHDPSH
                     WRAEVCESYPDIDLVLSGHTHGMQFGVEIPGLKWSPVQYVYNEWAGLYEKGNQKLYVN
                     RGFGFIGYPGRVGILPEITVLEFV"
     gene            9528..10331
                     /locus_tag="PLO70_12245"
     CDS             9528..10331
                     /locus_tag="PLO70_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQZ75283.1"
                     /translation="MARIIGVANQKGGVGKTTTAINLAASFAVLEYKTLLVDGDPQAN
                     STTGVGFDLHNVTQSLYDCMVNQARPKDVILKTEIPNLDLIPSHIDLVGAEIEMINYP
                     NREMVMKNLLESIRNEYDFIIIDCSPSLGLITVNALTAADSVVIPVQAEFFALEGLGK
                     LLNTIKIVQSRLNTALVIEGILMTMYDGRLRLCNQVVSEVRRHFDDMVFSTIVHRNAK
                     LAEAPSAGKPVILYDASSKGSMNYLNLAKEILQKNDMTKIKNEERILEL"
     gene            10337..11254
                     /locus_tag="PLO70_12250"
     CDS             10337..11254
                     /locus_tag="PLO70_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQZ75284.1"
                     /translation="MSTPKQNKELLGKGIRSLLQSIDTDLKTSSGHLKTTVVENVTGT
                     NRIAIDEIETNPKQPRRDFDEKAMQELADSIRLHDIIQPVTVSRLANGKYRLISGERR
                     LRASKLAGLKDIPAYIRQANDAQLLELALLENLQREDLNAMEVSLSYKRMMEELDYTQ
                     EQVAERMGKDRSTVANFIRLLKLPPDIQLAVRNGELSMGHARALINVDTVDKQLFIFK
                     EIKEKGLSVRQTEALVRNMYKQGGAVKKSSKSHLSPAFQRIEDKLASHFSTRVKLRNS
                     RNGSGQIIIDYYSQEELNKILGQMDAQLD"
     gene            11261..11890
                     /locus_tag="PLO70_12255"
     CDS             11261..11890
                     /locus_tag="PLO70_12255"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HQZ75285.1"
                     /translation="MRIFFSFICFLFLLSPALVHAQEKEPVAISTKDSTPVIKKTIAS
                     SNRYDSAMKAHSPKKAALRSAILPGWGQVYNKKYWKVPIVYGALGVCGGVFFYNMGNY
                     KDTRFAYRVKYNMRVNGTDSALFSQIKPKLKPLDEESLRFYRNQFRRDIDYSALFFLL
                     LWGLNVIDATVDAHLKSFDVSPELSLRVKPGYSDMARTNGLSLVMKIGK"
     gene            12021..>12499
                     /locus_tag="PLO70_12260"
     CDS             12021..>12499
                     /locus_tag="PLO70_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ75286.1"
                     /translation="MRIALIGYGKMGKAIEEVAIQRGHEIVLKIDQPNLHEFTKEHMA
                     TADAAIEFTGPYSAFNNVKKCIDFYIPVVCGSTGWTDKLDEIKKICTEQHGSLVYSSN
                     FSVGVNIFFEINKKLAALMAPHKEYEVILEETHHTQKKDAPSGTAITLAEQIRQEVK"
BASE COUNT         3826 a         2697 c         2373 g         3603 t
ORIGIN      
        1 cctttacaaa atattttaga atcgttgaaa caaaggctat taccggctcc ggaagggtac
       61 tctaccggat gactaaaaat gaaaactaaa gctttacata tactaaggac aaggccaata
      121 tttctttacc tgctgccagt cttctttgta cttcatggct acacagaaaa ctatgatttt
      181 gtgccggtaa atgatgcagt tattttactg gcaacctatt taatcgcatc actgattctt
      241 tcgttactgg catggatctt ttaccggaat ttcaccaaag caaacctgct ggcattttgt
      301 atgatggcat tccatttctt ttttggagca gtacaagatc tgatcaaaaa aatatctcca
      361 gatattttta ttttaaaata cagttttatt ctgccctgca ctgcgatact atttatagta
      421 ctgctgatca gtttaaaaaa aacaaaaaaa tcatttctta agattaccct ttttacaaac
      481 ctgctcttat tattatttat actagccgat tcaggctggc tcttactaaa agttttaact
      541 gtaaaagaaa taaaacgggc cccggtatcc ggacttttta aaaaatgtga caattgtaaa
      601 taccccgatg tctacctgat catggcagac ggatacccgg gtcagactga actgaaagct
      661 gtactggggt acgataacca attatttgaa caggagctcg agaaaagagg tttccatgtt
      721 atagacagca gcaggagtaa ctacaatctg accccttttt cagtcgcctc catgttggat
      781 atggattacc tgaaaggaat tgcaggacaa tacagcaata aaaatgatat agcactttgt
      841 tataacacga ttaaacaaag taaactattc agttttctga aacaaacagg ttataaaatt
      901 tacaactatt ccatatttga tcttaccaat caaccctctc ttgcaacgcc aaccttctta
      961 ccccgcaagt caaaaccgat cactgcacag acctttatat caagagccga gaaggaaatt
     1021 ggttttcatc ttgcaacaac acttaaaata aaatcaatta tccaatacgt cagaaaccat
     1081 gatcttaaaa ataacaggaa gctaactgcc aaaaccataa aaatcgccaa agaagcaacg
     1141 tcaaatccca aattcgtata cacacatctc attatgccgc attacccgta ttattttgat
     1201 agtaccggcc aggcagtatc atatgaaaaa attacagatg attttattgt agaccagggg
     1261 gcaatgatca gttaccttaa atattcaaac accaaactta ttgagttagt tgatcaaata
     1321 aaatcatccg ctgcctctcc accaattata atactcatga gtgatcatgg tttcagagaa
     1381 tttaaagtac cggtagataa taaataccat ttttataacc tcaatgctgt ttttttccct
     1441 gatagcaact attccctttt ctataatgga atgaccaata tcaatcagtt ccgggttata
     1501 cttaattcac aatttggcca gcaactgcct ttactgaaag attcgaccag ctttttgctg
     1561 gaataaataa aaagaaagat gcaatagact atttttgcca ccatgtttga tccgcaaaaa
     1621 atactggtgc ttgctcccca caccgatgac ggagaactgg gctgtggcgg aactatcgca
     1681 aaattctgtg cggagggccg ggaagttttt tacgctgcct tttgtctttg ctccaaatca
     1741 ttaccatcca atttacctgc tgatacactg gaacaggaat gtaaaaaagc tacagaatta
     1801 ctgggcatca ggccatctaa cctggtttta tttaattatg aagtgagaga acttcctcaa
     1861 tcaagacaaa aaatactgga agagttgctg caattaaata aaaagatcag tcccgacatg
     1921 gtattgttgc cggctgcatc ggatgtgcac caggaccacc aggtgattca ccaggaagga
     1981 ttacgggcat ttaagaatac tacttttgcc gggtatgagt taccctggaa taattacagt
     2041 ttccatacca actttttcat gcggttatcg gaaaccgaac tcaataaaaa ggcagcagca
     2101 ttaaaagcct accaatcaca gtcacaccgc aattatatgc aggaagattt tatacgttcc
     2161 ctggccaaag tgaggggtgt gcagtgcaac agtgagtttg ccgaagcttt tgagatatat
     2221 aaactgatca gctgatatta ccagccaatt ctttctccga attttcccaa tccaaaatat
     2281 tcattcagtt tcatattcaa acccagttgg ttgaaagcag aattatgctg gtaataagac
     2341 ctggcatagc ggatacttaa ttttcccagt ttcactcctg cccccataga aaacccattc
     2401 attccattcc ctgcattgcc aatattcaat tctttgcgcc ggataaaatt ataacctccc
     2461 tgtacttcta ccctgtcgcc aatatagatc gttgttgaaa gtataagatg attcaacagt
     2521 ttatcaaatg aaaatttatt gctgctactg ttcccgtaac cattttcatt attgaatgca
     2581 gtgtcgttat acaatatgtc aaattgctga agacgctgtg cagtcaatga aaaactgaaa
     2641 ggggctttct ctaatcgctt ggtaatgcca gcctgtaaat caaaggggag ttcctcctgt
     2701 tctgcattat tatatctttt gagttgaaca cccaggtttt tcactaaaaa agaggcagaa
     2761 aaaagccttg ttgtatcact aaacaaaacg ccgatatctg ttgcaatacc attcgaccgg
     2821 aactgcccgt aattcgaact gatccacttt agcgccgcac cgtaattcca tttctccagg
     2881 taactgcggg aggctgatac ctgcatcacc caatcggtcg gcctgaactt tccaaagaca
     2941 tttcccccgg catctgtttc ggtggtgctt ccataattga aataattaag tccccacaaa
     3001 aaatttgtct tgagtttttc attccggtaa cctaacgata aatgaaagac tttaataccg
     3061 ccataaaaat cattgaaaac cgcattcatt tgtgaatgca tccggggcgt aagtaaggca
     3121 gggttattaa aagccaggcc aatatcatct gattgttgag aaatattgac accacccagg
     3181 gctgttaatt gtggtgtatt tgaaagtctt aaaaagttaa acaccgaaag gcctcccaat
     3241 acctgggcag aacaatatcc ggatacaagt aaaaataata tcgtaaagcg cagaaacaaa
     3301 ccactacgaa aataagcgat atactacttc gctttttagt ctacaacacc tgttttaatt
     3361 tttacctgta aagaacatcc tttaccgggg ttactattac cggtcttata cagcgaaata
     3421 aaaagaaaat cctccctgaa aagggaggat ttcttaagtc cgcaatgcgg aactaacctt
     3481 aaacaaccaa catgcgaata ataaaaacat ccggcattaa aattggttga cgaaatacct
     3541 gaaattattt tgaaaccagg gccagttcca aaacctggtt catcgtcttt acataatgaa
     3601 acttgatacc ctttataaat tctgactcaa tctcctccac atctttctca ttttgcgagc
     3661 agagaatgat ctctttcaat ccggcccttt tagctgccag cactttttct tttattcccc
     3721 ctaccggcaa cacctgtccc cgcagggtaa tctcgccggt cattgccagg aaaggtttta
     3781 cttttttccc ggtcatcgca gaagcgattg ctgtcatcat cgtaacaccg gcactgggac
     3841 catccttagg aacagcacct tcgggtacat gaatatgaat attcttttta tcaaaatact
     3901 tagggtccag tttatacctt tttgcattag actgtaaata cgtcaacgca gtgctggcac
     3961 tttctttcat cacattaccg agattgccgg taagctttag ctcgcctttt ccatcactca
     4021 ggcttgtttc aatgaataga atttcacccc ctacatatgt ccaggcaagg ccaattgcca
     4081 ccccggccat attggctatc ttataaatat cattgctgta acggggcttg cccaatattt
     4141 gttcaatatc atccggcccc aagactgtct taactttttc ttttagtgcc agttctttgg
     4201 cctggtaacg catgatagaa gccagttgcc tgtccagttc cctcacacca ctttcccggg
     4261 tataatcctg gatgatcttt tccagcatct tatcactgat acggaaattg gtattcttta
     4321 acccatgcag gtctttctgt cttggtaata aatgccgctt ggcaatctct attttttctt
     4381 ccacggcata tccgctcagg tcaattattt ctaacctgtc tctcaatgcc ggctgaatat
     4441 tctgaatatt attggcagta gcgataaata aaaccttgct caggtcatat tccaattcca
     4501 ggtaattatc atagaaagta taattctgct ccgggtccag tacctccaat aatgctgaag
     4561 aagggtcgcc acggaaatca tttcccactt tatcaatctc atccagtatc atcaccgggt
     4621 tagatgattt tattttacgg atcgattgca ggatacggcc gggcattgct ccaatatatg
     4681 ttttacgatg tccccttatt tcactttcat catgcaggcc gcccaggctt aatcgtacat
     4741 atttcctgcc aatggcatta gcgatactcc ttcccaatga tgttttacca ataccgggag
     4801 gtcctacaaa acaaagtatc gggcttttca tatcaccttt cagtttcaat actgccaggt
     4861 attccaggat ccgttcttta attttttgca tgccgtaatg gtcatgatcc agtgtttctt
     4921 ttgctttctt cagatcatag tgatcttcgg tatattcttc ccagggcagg tcaagcatca
     4981 ggtcgagatg attatacacc acagaaaaat caggtgtgct tggatgcatc cgttctaatt
     5041 tttcaacccc ttttttaaac atttcttttg cagcagccgg ccattttttt gcttccgcct
     5101 ttttctgcat ttcttttatt tcctgtgaat tgctatcacc acctaattct tctttaatac
     5161 ttttcagttg ttgctgcaga aaatattccc gctgttgttt atccagttcg gtccttgttt
     5221 tggtagttac tttatttttc agttcggcaa actgcaattc cttttgcaaa agctgcatca
     5281 gcaggtcagc cctttgacgg atctggttta actcgagtaa ccgttgtttg tcctttatgt
     5341 ctgtattaag attactggat acaaagtgta tcaaaaagga agggttctca atgtttcgca
     5401 gaatgatgga ggcttctgta ggaatattag gcgatagctg tacgatatct gctgccaggt
     5461 ccttaatatt ggcaacatat gcattaaaat cttcatcctt cggggattcc tcttcttcca
     5521 gctttgtaat ttttgccttg aaataagggt catcttcgaa aacagtatca atctggaacc
     5581 ggctttttcc ctggataata atagtggtgc ctccatctgg cattttgatc attttgacga
     5641 tcttggccac ggtgcccact ttttccagat ctttaatttc agggtcttcc acattgctgt
     5701 ctttttgcgc tatgactcca atcaatttgt cagtcttata agcatcattt acagccttta
     5761 tactcttatc ccggccaaca gtgatgggca acaccactcc cggaaaaaga accgtgttcc
     5821 gaaggggtaa aagagctatt tccgccggaa tctctacacc attcgggtct tcctggtcac
     5881 cttcattcaa cggaatgatg ggtaaaaagt ccatttcatc ttctgaacgc aacatatatt
     5941 tttcaatatc catagtcata tcttctttag acaatctgtc atccccaaag cgggaacggt
     6001 aaaaatagag tgtgaaatca gtattgccaa gattcgtgcc tgtcaattaa aaactgccaa
     6061 actatgtagt tgagaaggag ctgtttacaa taaaagaagt cccccaaatg gaggacttct
     6121 ttttattccg gaatgtgatt ttagagggct agtccaacgg atacctggaa accctggttt
     6181 ttccatttgt catcatcagc tatatcgctg atattattta agccaatagc ataccggcca
     6241 tttatacgca tgggaccaac tcttaccagc acaccagcca gcatcgaaag atctccgctc
     6301 ttgaaggcat tgcccccgtt ctgaagcaat gttttattct ggtctaataa tacaccaaat
     6361 tgtggccctg cctgcaggga aataaaatta ccgaccagtt tatagttcaa cagaaccggc
     6421 acagagaggt aattgagttt tatattactg ttgccgtcaa atacatcttc atatacattc
     6481 ttgtagttac tgtccagcct ggtggcatat tggttgaaaa gaacctctgg ttgcagaaca
     6541 agtttattac ccaacctgat ttcagcaaaa ccaccaagat gataaccata cctgaattca
     6601 tctttaaaag actttccatc tactttagtg atattagcac cggctttaac gcctaagtga
     6661 aactgggcca tcatagcctg ggagagtaaa actgctgcga agagcgatag gaattttgtt
     6721 ttcatgtgtt aatagttttt atgtttttgt ccaatggttt caggacagca aaaaaattca
     6781 agtatagtgc cagtaaccct tgccgcctgt taaatttcat taacgacaga taagtaaatc
     6841 cgtaaactac atatgtaact attctattgc ggataaaatg tgaagatttt ctaaaatgca
     6901 aatatatcag aaaaaaaatc cagcattcaa tgaaaacaat gtattgaagt ttgctgatgt
     6961 agccggaaaa taataatcca gtatcagctg tggttgtaaa aagaatttac cgattgagta
     7021 ttcaccccgg agatataaac tgagagaaag cggttgaaaa tttatctggt catcaagata
     7081 aacattatcc gaattgaaaa gaatattact gcccgtgcgg atggtagtag cataggtggc
     7141 agatgactgg ttgaaaccga atttttgcgt gccgcttgta aaagtcaatt gcggggtaaa
     7201 gcggatatgg tctttcaggg taaatatatc aagccagtaa aagtcatgcc gcaccgaagc
     7261 catcagggaa aaatcactca cagtttcttc tgtgtttata taagtgaagc cccttctccg
     7321 caaacgaaga tcctggatca gttcttcccg ttgcatataa tcactgcggc ttccccatcc
     7381 atagctcaac gccagggacg gctttaccca ggattgacgg taaagaaaat aagcatacaa
     7441 ttcattttgc agcggggtag tataaaaagg caaggagtct ttggtaaagt atcttgtata
     7501 agaaatacct gtagcaaatt ttcgattctg caaataatca taggaaggag taatacccga
     7561 ttggtaaaaa ttcatattct tcccgtcatc tacaatataa ccgattgccg ttagccccag
     7621 gccaccttta tgataatatc ctaacgtagg tgagtaattt aattttttgg aagtcgtaac
     7681 agtgtaattg cttttattgg caaaattgta ataccctttt cctacagaca ggctactgag
     7741 aaaataactc cgcggcgtga gaattgaatc caaaaacgca tcaaaatccc ggaagagttc
     7801 atcataatca atactggtat ctatcagtga tacttctgca actggtataa tagttgaatc
     7861 accagcgttg ctctgcgaat gtgcagcaac actgccaata ataaaggcca ggagtataga
     7921 ccattttttt agcatcaggc agttgtggtc ggcttgttaa atagatgaag aagccttaat
     7981 tgtcgtctta agactaaaag gttaaaaaaa agtttaataa gacacttttg aagaacgaaa
     8041 aggggcaaaa attattgagt cgcgaagaat aatttgtttg acttacaagg ctttacatcc
     8101 tgaattgagc ttacacaaac tccaaaacag tgatttcagg caatataccc accctgcccg
     8161 ggtagccaat gaatccaaag cccctgttta catacagttt ctgatttcct ttttcgtaca
     8221 gacctgccca ttcattgtat acatattgaa cagggctcca ttttaaaccg ggtatctcca
     8281 ctccaaactg catcccatgg gtatgacccg atagcaccag gtctatatcc gggtatgatt
     8341 cacaaacctc ggccctccag tgagaagggt catgactcaa caaaatttta aaaggatgct
     8401 tttcagaacc agcataggct tttttcaaat caccatattt aggaaacctg gctttagaac
     8461 tccagttctc aatgcccaac agggcaatac tgtccgattc tttttcaagc actacatgct
     8521 cattcattaa taacttccag cccattgcag catgaacctg tttcagtctt tcaagattag
     8581 tatttttttc ttcaggacta tcccactcta cataatcacc ataatcatga ttacccaatg
     8641 tagaataaac accaagcggt gcgttcagct tattgaacac atccatatat tcatccattt
     8701 cagaagccac attattcacc aggtcaccgg taaataggat gaggtctggt ttctccagca
     8761 tgagcttttg cacgcctttt atcacggctt ccttatcgga aaaactgccg gcgtgaatat
     8821 ctgatatatg agcaattttg atacccctga atgcttgcgg tagattggga taatcgagtt
     8881 ttatttttgt aacctggtac cggtgtttgt taccaaagcc ataaataagt gtaccaaaca
     8941 acccgctgcc ggccagcatc cctgcccaac tcaaaaaaac ggaccgggat attccatcac
     9001 caaccggtgt aatgcctgcc tctttgggtt taaaaaataa tttagtaccc gcccattgta
     9061 tgcctcttct tacatcatcc accacaaaga acaaagaagc gatgagctta gcaaaaaaaa
     9121 gaccggctat catcgcaaaa atggtactct tggccagttt cgcctgctta tcaaaatgca
     9181 ggtaaggcaa caacaaaagc atcaggatgg ctcctattga aacaatccaa tagccgctat
     9241 ggattaacag ccgggtacgg gaagacgccg aatgggtaat tactttcagc acctggaaga
     9301 aatatacatc caacagcacc ataaaactta taaaaatcca ccaaaaggga gagttacgca
     9361 taaatagatt ataaaattaa gccggtaatc ataataggca gtcttttgtc gaacgcttat
     9421 ttttgccgga atgcccgata aatgggcaaa acttaatttt gcgttcttag tttcagtcgg
     9481 caattttacc actaacaatt cattaatctc aaggagtttt cggcaaaatg gcaagaataa
     9541 tcggtgtagc taatcaaaaa ggaggggtag gaaaaaccac tactgctatt aatttggcgg
     9601 caagttttgc tgtgcttgaa tataaaacat tgctggtgga tggcgacccg caggctaaca
     9661 gtaccacagg tgtaggtttc gatttgcata atgtcactca aagtctttac gattgcatgg
     9721 tcaaccaggc caggccgaaa gacgtgatat taaaaaccga aataccgaat cttgacctga
     9781 tcccttcgca tattgacctt gtaggtgctg agattgaaat gatcaattac ccgaaccggg
     9841 aaatggtcat gaaaaatttg cttgaatcca ttcgcaatga gtatgatttt atcatcatcg
     9901 attgttcgcc ttcattagga cttattaccg ttaatgcttt aacagcggct gactcggtcg
     9961 ttatcccggt gcaggctgaa ttttttgcat tagaaggact cggtaaacta ttgaatacaa
    10021 ttaaaattgt tcaaagccgc ctgaatactg cacttgtaat agaaggcatc ctgatgacaa
    10081 tgtacgatgg ccgccttcgt ctttgcaacc aggtggtaag tgaggtacgc cgccattttg
    10141 atgatatggt tttcagtact attgttcatc gtaatgcaaa actcgctgaa gcacccagtg
    10201 ccggcaaacc ggttatactt tacgatgctt ccagtaaagg ttccatgaat tatcttaacc
    10261 ttgccaaaga gattttgcaa aagaatgaca tgacgaaaat taaaaatgaa gaaagaattt
    10321 tagaactgta atgcaaatgt caactccaaa acaaaataaa gaattgctcg gcaaagggat
    10381 ccgctcctta ttgcaaagta tagatacaga cctgaagacc agcagtggcc atttaaaaac
    10441 aacagtggtg gagaatgtta ccggtaccaa ccgtatcgcc attgatgaaa ttgaaaccaa
    10501 ccccaaacaa ccacgtcgtg attttgatga gaaagcaatg caggaactgg ctgattccat
    10561 caggctgcat gacatcatac aaccggtaac tgtttcaaga ttagccaacg gtaaatatcg
    10621 cctcatttcc ggggaaagaa gattaagggc atccaaactg gcaggcctta aagatatccc
    10681 agcgtatata agacaggcca atgatgcaca gttgctggaa ctggccttat tggaaaacct
    10741 gcaacgggaa gacctgaatg caatggaggt atcactcagt tacaaacgga tgatggaaga
    10801 actggactat actcaggaac aagtggcaga gagaatgggt aaggacagaa gtacagtggc
    10861 taattttatc cggttactga aattaccgcc ggatattcaa ctggcagtca gaaatggtga
    10921 actaagtatg ggccatgcaa gggcactgat caatgtagac acggtagaca aacaactttt
    10981 cattttcaaa gagataaaag aaaaaggatt atcggtacga caaaccgaag cactggtgag
    11041 aaacatgtat aaacagggcg gtgctgttaa aaaatcttca aaaagccatt tatctcccgc
    11101 ctttcagcga atagaagata aattagcatc gcatttcagt acccgggtaa aactcaggaa
    11161 tagtcgtaac ggaagcggac agatcatcat tgattattat tcgcaggaag agctgaataa
    11221 gatcctcggg caaatggatg cacagcttga ttaaatcacc atgagaatat ttttctcctt
    11281 catttgtttt ttgttcctgc tttccccggc tttggtccat gcacaggaga aagagccagt
    11341 tgctatttcc acgaaagata gcacaccggt tataaagaaa actattgcca gcagcaacag
    11401 gtatgattct gctatgaaag cacatagtcc caaaaaagcg gctttacgtt ctgccattct
    11461 tcccggctgg ggccaggtgt ataataagaa gtactggaaa gttcccattg tatatggagc
    11521 attgggtgtc tgcggtggtg ttttctttta taatatgggt aattataaag acacccgttt
    11581 tgcctaccgg gtgaaatata acatgcgggt gaatggtact gattctgcat tattctccca
    11641 gatcaaaccc aaactaaaac cccttgatga agaatcactc cgcttttacc gtaaccagtt
    11701 ccggagagat attgattatt ccgccctctt ctttcttctc ctttggggct tgaatgtaat
    11761 tgatgccact gttgatgcac accttaaaag ctttgatgta agtcctgaac ttagcctgcg
    11821 ggtcaaaccc ggctacagtg atatggcaag aaccaatggc ctgagcctgg taatgaaaat
    11881 aggtaagtaa taaatccgta ccagcacggg tagctgcacg aaccattaat aactacattt
    11941 gtaacagatc aaaattatca acctgttttc agcctgaaaa gactgaaagt tatccgcata
    12001 cagcttttaa aaagacttat atgagaattg cattaattgg ctatggtaaa atgggaaaag
    12061 ccattgaaga agtagccata cagcggggac atgagatcgt tttaaaaatt gaccagccca
    12121 acctgcatga gtttaccaaa gaacatatgg caacagcaga tgccgctatt gaattcaccg
    12181 gtccttacag tgcttttaac aatgtaaaaa aatgtattga tttctatatt ccggttgtat
    12241 gtggctctac cgggtggaca gacaaactgg atgagataaa aaagatctgt acggaacaac
    12301 atggctctct tgtttactcc agtaatttca gtgtgggggt gaatattttc tttgaaataa
    12361 ataaaaagct ggccgcactg atggccccgc ataaggaata cgaagtgata ctggaagaga
    12421 cccatcacac acaaaaaaag gatgccccca gcggtaccgc cattacactt gccgaacaga
    12481 tacggcagga agtaaaaag
//
LOCUS       DAORTI010000103        12489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5065_length_12489_cov_5.364967, whole genome shotgun sequence.
ACCESSION   DAORTI010000103 DAORTI010000000
VERSION     DAORTI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_12489_cov_5.364967"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1249
                     /locus_tag="PLO70_12265"
     CDS             29..1249
                     /locus_tag="PLO70_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQZ75287.1"
                     /translation="MKFLAFIFLAVNFSFCNSLKNQSQHAVDPVNGSIKTGAEQTDKY
                     LPYLKGKRVAIMANPTTIIGKTHLVDSLKTLGVNIVKVFGPEHGFRGNASAGAHVADE
                     TDPATGIPVISLYGKKNKPTREDLADVDVLIYDLQDVGCRFYTNINALARLMDACHEN
                     GKEMLILDRPNPNGYFVDGPVLDMKYKSGIGMFPIPIAHGLTVGEFAQMLNGEGWLPG
                     KVKCTIRIIPVANYTHDMAYTLPVKPSPNLNTQQAIMLYPSTCLFEGIYLNHGRGTYF
                     PFTIIGSPELKGKYEFSFTPTGIKGMAETPLFIDQVCYGLDLRNVDIEQLRKSKQINI
                     NWIIELYKAHPYKEKFFDSKLSNQMGTIEKLVGSGLFRQQIIDGKSEKEIRDSWEPGL
                     SDYKQMRKKYLLYP"
     gene            complement(1338..2030)
                     /locus_tag="PLO70_12270"
     CDS             complement(1338..2030)
                     /locus_tag="PLO70_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQZ75288.1"
                     /translation="MKKRISLILVMALGSLFSQAQQTVKNSGGTSFGLRAGVNFQNIT
                     GKDEDGNKLENDLLTGFNIGINAEIPLAPQFYFQPGLLFTTKGAKSEDLILGQTIKGK
                     INISYVEMPLNFLYKPMLGQGRLLMGFGPYVALGVGGKATYEGGGSSLSEDIEFKKTV
                     KLSDPDDVFYVRPMDAGANLLFGYQFANKVSVQLNAQLGLTKINPEYEGASNDRTEAK
                     NTGFGISLGYRF"
     gene            complement(2101..2793)
                     /locus_tag="PLO70_12275"
     CDS             complement(2101..2793)
                     /locus_tag="PLO70_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ75289.1"
                     /translation="MKTFLISLLMLAIFSAGCKTMNKSQKGAVIGTAGGGAVGAVIGK
                     ATGNTLLGAIIGATVGGVAGAVIGNKMDKQAEEIKNEIPDAVVVRVGEGIVIEFNNKI
                     LFGFDQSTLSPESRTSLDKLVVILQKYPDTNIEVQGHTDSKGSIRYNQDLSERRALAV
                     ASYLSGFGISPSRVVIAPARLTTIGFGESLPKYTNTTTDGQAQNRRVEFLVTANEKMK
                     ADAAREATKNGG"
     gene            complement(3002..3808)
                     /locus_tag="PLO70_12280"
     CDS             complement(3002..3808)
                     /locus_tag="PLO70_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQZ75290.1"
                     /translation="MKKFILLLLVSALFVSCTENLVTGRKQLSLVSETELQTLAKQEY
                     QTFLTDNKVVNVSNNRDAEMVRRVGNRIASAIKTFYDGKGMTTVLEGYQWEFNLIDNK
                     EANAWCMPGGKVVVYTGLLPITQNEAALAIVLGHEIAHAISQHGSERMSQALLQQLGG
                     VALQVALANKPAETQNLFLSAYGIGSSVGVILPFSRKEESEADKYGLYFSAMAGYNPQ
                     EAIPFWERMSAAGGAKPPEFLSSHPSDASRVEKIKTYMPQALKYYRPVNK"
     gene            complement(3882..4247)
                     /locus_tag="PLO70_12285"
     CDS             complement(3882..4247)
                     /locus_tag="PLO70_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ75291.1"
                     /translation="MSSDYKNELPENTELEKSKAHIIVEIIEYMANSVVIKTIIKKST
                     GNISVMSFDSGEGLTEKTTPFDTFVQIIEGKADIVIDNVSNLLQSGQGIIIPAHSPNL
                     IRPNGRFKMISTIIKSGYE"
     gene            complement(4349..4789)
                     /locus_tag="PLO70_12290"
     CDS             complement(4349..4789)
                     /locus_tag="PLO70_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75292.1"
                     /translation="MKTKNSVNLSKGIFSGILLIAMLFLFDSCARKIVFQSSSVVPAA
                     EGTVKVTKDNNSNYAIHLQLSNLAEPEKLTPAMKTYVVWMETAQENAKNIGQINSSTS
                     LLSKRLKASFETVSPVKPTKIFITAEDNATVLYPGTVVLTTDNF"
     gene            complement(5089..5244)
                     /locus_tag="PLO70_12295"
     CDS             complement(5089..5244)
                     /locus_tag="PLO70_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HQZ75293.1"
                     /translation="MSNLLYLVAVILIIGWVVGYFGFNLGGIVHLLLVIALVMIVARL
                     IQGKRAV"
     gene            complement(5366..5551)
                     /locus_tag="PLO70_12300"
     CDS             complement(5366..5551)
                     /locus_tag="PLO70_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HQZ75294.1"
                     /translation="MNTTELKGNWNETKGKLKQKFADLTDNDLMYTEGKKDEMLGKLQ
                     IKLGKTKEELHKIIEAL"
     gene            complement(5825..6385)
                     /locus_tag="PLO70_12305"
     CDS             complement(5825..6385)
                     /locus_tag="PLO70_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ75295.1"
                     /translation="MKLYIKNMVCIRCKMVVKEELRKLGLHYTVVDLGEANILENITT
                     EQHDQFKTALLKSGLELMDDKKSVLIQKIKNIIVELVHYSEEPLVLKFSEYLSQKLNH
                     DYTYLANLFSEVQGTTIEKYLIAHKIERVKELLIYDELNLTEIAYQMHYSSVAHLSTQ
                     FKKVTGLTPSYFKQLKSKRRTMLENV"
     gene            6917..7225
                     /locus_tag="PLO70_12310"
     CDS             6917..7225
                     /locus_tag="PLO70_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75296.1"
                     /translation="MTAKNYLRMASLALFIAIASPASSAAIVPESITPTEDVRSQQLL
                     QRLEEIKGLDKSEMSRLEKKSLRKEVKEIKKEMKALSGGVYLSVGAIIIIILLLILIL
                     "
     gene            7316..7693
                     /locus_tag="PLO70_12315"
     CDS             7316..7693
                     /locus_tag="PLO70_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75297.1"
                     /translation="MKSLLFVVSLFSFIMYRASRSDIPLKGTWDGSYRTIEKKMDLRI
                     VFGPGNEVQLYSTEIDNKGKAVGTYAVKRNEIAITCIWPDSDSIPFIMKGRLNSNQNF
                     VDGNWESADKTDGNFYLAKTVSK"
     gene            7892..8077
                     /locus_tag="PLO70_12320"
     CDS             7892..8077
                     /locus_tag="PLO70_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75298.1"
                     /translation="MPLLTILLVLIIAGVILWLVNTYIPMDRKIKNILNIVVVIIVVI
                     WLLQAFGLLDSLKSIKV"
     gene            8511..8705
                     /locus_tag="PLO70_12325"
     CDS             8511..8705
                     /locus_tag="PLO70_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75299.1"
                     /translation="MNIKRIFGALLTILGTAGLIYGAVLFINTPGGTRDIKALAIYGI
                     LGLIFFIAGINLIRTTKDES"
     gene            8813..9088
                     /locus_tag="PLO70_12330"
     CDS             8813..9088
                     /locus_tag="PLO70_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQZ75300.1"
                     /translation="MKSGKVLLGVLAGLATGAALGILFAPDKGSATRKKISKKGEEYA
                     DKFGEKYNEFITGIKNKFVTAKEEAVQLAENGKAKAEEIAAAAKNRM"
     gene            9165..9509
                     /locus_tag="PLO70_12335"
     CDS             9165..9509
                     /locus_tag="PLO70_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75301.1"
                     /translation="MNESIVTPIEELIEKAGEYGKTSIELLKLKAIDKSVSVISSLAV
                     KTVLLIVVAMLILSISIGVALWTGELLGRPYYGFFVMAGFYALATIVLFLFRDQWIRK
                     PVNESMIKKILQ"
     gene            9518..9937
                     /locus_tag="PLO70_12340"
     CDS             9518..9937
                     /locus_tag="PLO70_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75302.1"
                     /translation="MKTINSTSELTEAILLLETRQADQGIQLREQFNLTYESLKPLNL
                     IKSTFREAASSRELKDNIINTGTGLVAGYLSKKLFEGVSHSPLKKILGSALMFGITNM
                     VSRNPETIKSLGQKFLNGISFKPVDKNVNRNDKNEIF"
     gene            complement(10009..11646)
                     /locus_tag="PLO70_12345"
     CDS             complement(10009..11646)
                     /locus_tag="PLO70_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HQZ75303.1"
                     /translation="MEDFKIVVFIMAILISLTAIANKRKLPFPVLLVAAGLIIGFVPQ
                     LPDLALDPEVVFVILLPPLLYDAASKTSWHEFRTSIRPISALAITLVFFTTVAVAITA
                     HFLIPGFSWPLAFVLGAVVSPPDAVAASGIIKGLGLNKKVITILEGESLVNDASALIA
                     YRYAVTAVTTGSFVFWQAGLQFLLVAGVGIIIGIVIGYLFVFAHKKIDSNPVVETSLT
                     LLSPFVSYLAAEQFHMSGVLAVVSTGLVIAWRAPEVFSYQARMRTRVVWDTLIFLLHG
                     FVFILIGLQLPSIIKGLGNYPFSQILGYGLLISLVTIIVRIVWVFAGAWSQNYFQKRK
                     KKTGVIAGKDDSDSTWKNVLVVAWTGTRGVISLAAALALPLVLEDGTAFPKRHSIIFL
                     AFVVIFVTLVVQGLSLPLLIRWLKMKPQDNTEEEEKELQLYLANSTLHFIEQDFPVPL
                     DNKSEQQLKMKYEVLINELTKEINRHKKAKRNDSEVMAKQQGVLLSAQLEISKFQRAL
                     LIKLHKEGEFSDAVIRQVEQEMDINELKLNLQVSKEE"
     gene            complement(11841..12422)
                     /locus_tag="PLO70_12350"
     CDS             complement(11841..12422)
                     /locus_tag="PLO70_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQZ75304.1"
                     /translation="MKFYFSAVVIAILITGTVNAQHINVGVKGGLNLYNINNDNGTKY
                     DTKAGIHLGLLGHIHLAKQLALQPELVYSAQGAKYSVGSVETKYKLGYINVPVLVQYM
                     FDNGFRLQAGPQVGFLVSAKADVNGNSVDIKDDLETVDFALGIGAGYVNPGTGFGIDA
                     RYNLGLSNINDNSTVKSTNRGFQLGVFYLFGHK"
BASE COUNT         3966 a         2538 c         2165 g         3820 t
ORIGIN      
        1 tatttattaa cacataatct gcgtttttat gaaattcctt gcgttcattt tcctggcagt
       61 caattttagc ttttgcaaca gtcttaaaaa ccagtcacaa catgctgtag atccggttaa
      121 tggctctata aaaaccgggg ctgaacagac agataaatat cttccgtatt taaaaggaaa
      181 aagagtggcc attatggcta atcccaccac tataattggt aaaacacacc tggttgacag
      241 tctcaaaaca ctgggagtta acattgtaaa agtttttggc cccgaacatg gcttccgggg
      301 aaatgccagc gccggcgctc atgttgccga tgaaacagat cctgcaaccg gtatacctgt
      361 tatttcttta tatggcaaaa agaataaacc tacccgggaa gaccttgcag atgtggatgt
      421 gctcatttac gacctgcagg atgtaggctg tcgcttttat accaacatca atgcactggc
      481 acggctgatg gatgcctgcc atgagaacgg aaaagaaatg ctgatacttg accgccccaa
      541 tcccaacggt tatttcgtgg atggccctgt gctggacatg aaatacaaat ctggtatcgg
      601 tatgtttcca attccaattg cacatggact tactgtagga gagtttgcac aaatgctaaa
      661 tggtgaaggc tggttgccgg gtaaagtaaa atgtacgatc agaataatac cggttgccaa
      721 ttatacccat gatatggcct atacattgcc ggttaaacca tcacccaacc tcaacacaca
      781 acaagctatc atgctatatc cgtctacttg tttgtttgag ggaatttatc ttaatcatgg
      841 ccggggtact tattttccat tcaccatcat cggcagtcct gaactgaaag ggaaatatga
      901 gttttcattt acaccaacgg gtataaaagg tatggcggaa acacctcttt tcattgacca
      961 ggtttgttat ggacttgacc ttcgcaacgt tgatattgaa caactccgca aaagcaaaca
     1021 gatcaatatt aattggatta tagaactata caaagctcat ccctacaaag aaaaattctt
     1081 tgattccaaa ctcagtaacc agatgggcac tattgaaaag cttgttggtt ctggtttatt
     1141 caggcaacag atcattgatg gcaaatcaga gaaggaaatc cgtgatagtt gggaaccggg
     1201 cctgagcgat tacaagcaaa tgcgtaagaa atacctgctt tatccatagg atgagtccag
     1261 cggaacaggc ggtaacaaaa aaatgagact gccttttgag acagcctcat gaagtgatca
     1321 tagtaggtta ttatttatta aaacctgtat cctaaagaaa taccgaaacc ggtatttttt
     1381 gcttcagttc tatcattaga tgcaccctca tattcaggat ttatttttgt aagccccagt
     1441 tgtgcattta actgtacaga aaccttattg gcaaactgat aaccgaatag caaattggct
     1501 cctgcatcca ttggtctcac atagaataca tcgtccggat cgctgagttt tacagttttc
     1561 ttgaattcaa tatcctcact caggcttgaa cctccacctt cataagttgc tttaccacct
     1621 acacccaatg caacataggg accgaagccc atcaacaggc gtccctgccc cagcataggt
     1681 ttgtacaaaa aattcaatgg catttccaca taagagatgt ttattttgcc ttttattgtt
     1741 tggccaagaa taagatcttc acttttagct cccttggtgg taaaaagcag tccaggctga
     1801 aaataaaatt gcggtgccag cggaatctct gcattgatac caatattaaa accggttaac
     1861 agatcatttt ccaatttatt tccatcctca tccttgccgg taatattctg gaaattcacg
     1921 ccggcacgaa gaccaaaact tgtaccaccg gaatttttta ctgtttgctg agcttgtgaa
     1981 aacaggctgc ctaaagccat tactagtatt aaactgattc tctttttcat tttattattt
     2041 tatttagtct tattatttat acctaaactt ctacccctgc ctgttatgtg tttaatttta
     2101 ttaaccacca tttttagtag cttcccttgc agcatctgct ttcatttttt cattagcagt
     2161 aacaagaaac tctactctcc tgttttgtgc ctggccatcc gttgtggtat tagtatactt
     2221 tggcagtgac tccccaaacc cgattgtagt aagcctggca ggtgcgataa caacccttga
     2281 aggcgaaata ccaaatccgg ataaataaga agcaacagct aaagcccggc gttcagaaag
     2341 atcctggtta tacctgatag atcctttact gtctgtatga ccctgcacct caatattggt
     2401 atccgggtat ttttggagga tcaccactaa tttatcaagg cttgtcctgg attctggtga
     2461 taaagtagac tgatcgaaac caaataatat tttattattg aactcgataa cgataccctc
     2521 tcctacccgt acaactacag catccggaat ttcattttta atctcttctg cctgcttatc
     2581 cattttatta ccaataactg cacctgctac accacccact gttgccccga tgatagcacc
     2641 aagcaatgta ttgccggttg ccttaccgat aactgcacca actgctccgc caccagctgt
     2701 tcctataact gccccttttt gggatttatt cattgttttg cagccagcag aaaatatggc
     2761 aagcattaat agactgatta gaaaagtttt cattttgttt gtttaagttg tgactggttg
     2821 atatatatta accaggatgt aaaggtggct ttaataaaag tggtaagtat tacacaactg
     2881 aagaattatg ttatatcatt cacacattcc gtaatacgta cctgtgatta atggagaagc
     2941 cgttgctata tagcccgata cgaagtaaaa aatgtaataa ttgacaataa aataaattac
     3001 tttatttgtt gacaggtcta taatatttca gtgcctgtgg catataagtt tttattttct
     3061 caactcttga tgcatctgat ggatggctac tcaggaactc aggtggcttt gctccccctg
     3121 ctgcagacat tctttcccaa aaaggaatag cttcctgtgg attataacct gccatagctg
     3181 aaaaatataa accatactta tctgcttctg attcttcttt acgggagaaa ggcaatatga
     3241 ctccgacaga gcttccaatt ccataagcag ataaaaaaag attctgcgtt tctgcaggct
     3301 tattagccaa ggcaacctgt aatgccacac cacctagttg ttgtaacaga gcctggctca
     3361 ttctttcact tccatgctgt gaaattgcat gggctatctc atgacccaaa acgattgcta
     3421 atgcagcttc attttgtgtg atgggcaaca aaccagtata tacaactact tttccgccag
     3481 gcatacacca ggcattagct tctttattat caataagatt aaactcccac tggtatccct
     3541 ctagtactgt tgtcattcct ttgccatcat aaaatgtttt tatagcagag gcaattctgt
     3601 ttcctaccct gcgtaccatc tctgcatccc tgttattgct cacattaacc actttattgt
     3661 cagtcagaaa tgtttggtac tcctgcttcg ccagtgtttg tagttcggtc tccgagacca
     3721 ggcttagctg cttgcgcccg gttaccaggt tttcagtaca actgacaaac agggcagaaa
     3781 ccaacagcaa taaaataaac tttttcattg tataagttat ttaagatatc aaaattgtcc
     3841 ttatcaatca gcattgacca ggacattatt cagcactata attattcgta cccgctttta
     3901 atgatcgtag agatcatttt aaaccggcca ttgggcctga taagattggg cgaatgagcg
     3961 ggaataataa taccctggcc cgattgtaat aaattggaga cattatcaat tacaatatcc
     4021 gctttaccct ctattatctg gacaaatgtg tcaaatggag tagttttctc tgttagtcct
     4081 tccccgctgt caaaagacat gacacttata ttgccggtag atttttttat aatcgttttt
     4141 atcacaacag aattcgccat atactctata atttcaacta ttatgtgtgc tttcgatttt
     4201 tccaattcgg tattttctgg taattcattt ttgtaatcac tgctcatact gaaattgatt
     4261 aagtgttcaa aatctaatat ttacgccttt taattcaacc aactataatt tcattacttt
     4321 tcttattgat gacatattta cagactattt aaaaattatc tgttgtcaga accactgttc
     4381 cggggtataa tactgtagcg ttatcttcag ctgttatgaa tattttagta ggcttgacag
     4441 gtgaaacagt ttcaaatgaa gcttttaatc tttttgaaag caaacttgtt gagctgttaa
     4501 tttgcccaat attttttgca ttttcctggg cggtttccat ccataccaca tatgttttca
     4561 ttgccggtgt caacttttcg ggttctgcca ggttcgacaa ctggagatgg atagcataat
     4621 tactgttatt gtctttagtc accttaacag tgccctctgc agccggaaca acagatgaac
     4681 tctgaaaaac gatcttcctg gcacaggaat caaataaaaa caacatcgct ataagtaaaa
     4741 tacctgaaaa aatacctttg gaaaggttta ctgagttctt agttttcatt tttttatcat
     4801 tttttaaatg tcaaaaactg acgatgtaaa ggtctctttc attctctttg atactgttac
     4861 acaactctgt taattagtta tatcattcac acattagttg aaataataaa tgcaggaaca
     4921 gcttcaatta ctatcaatat tttatacatg cccggatatg ataaaataat gagcccctgt
     4981 taaaacaggg gctcaaacca agaccaactg aaaataatta taaataaata caaaagacaa
     5041 taaagggttc taacacaaaa tatcgttgtg ctattgcaac caccaatatt aaacagctct
     5101 tttcccctgg ataagtctcg ccactatcat aaccagagca atcacaagta acaggtgaac
     5161 gataccgccc agattaaatc caaaatatcc aactacccat ccaatgatca aaataactgc
     5221 aacgagatat agtaaattac tcatattaat attgttaaac gccttataaa atatttaatt
     5281 taatactgaa actattataa actgtttaaa aagccagtac cggcaaatca aaaaccggga
     5341 ctagcttttt gataggaaat aattcttaca acgcttcaat aattttatgc aattcctctt
     5401 ttgttttacc caatttgatc tgtaattttc ccaacatttc atctttcttt ccttcagtat
     5461 acatcaggtc attatctgtc aggtctgcaa acttttgttt gagtttacct ttcgtttcat
     5521 tccaattacc ttttaattct gttgtattca tatatttaaa tttattgtga aatcattttc
     5581 acttgctaaa ggtggatcat tttatcacca actttattac atgatcacaa aaattaatta
     5641 tatgattcac acatctgctt tccgggaaaa tattgtagcc cttgccaatt acattctttt
     5701 cccacggaat actaaaatat attttactag ctgaaaaagg caacttaacc agttttctaa
     5761 cagaagctat gccaatccgg attttggtaa aaagctgcta gccttttatt gaatgtttag
     5821 ttaatcatac attctctagc attgtccgtc tttttgattt gagttgttta aaataagacg
     5881 gcgtcaaccc tgtgactttt ttgaattgag ttgaaagatg tgccacgctg ctgtagtgca
     5941 tctggtaggc tatctcggta agattgagct catcataaat cagcaactct tttacccttt
     6001 caattttatg ggcaattaaa tatttctcta ttgttgttcc ctgcacttca gaaaaaagat
     6061 tagccaggta ggtataatca tgatttaatt tctgactcaa atactcagaa aatttcagaa
     6121 ccaggggctc ttctgaataa tgcaccagtt caactataat gtttttaatc ttttggatca
     6181 gcacactttt cttatcatcc attaattcaa gacctgattt caataaagcc gttttgaact
     6241 gatcatgttg ctcagtggtg atattttcca ggatgtttgc ttcccccagg tcaacaactg
     6301 tataatgcag acccagtttt ctgagttctt cctttaccac cattttgcaa cggatgcaaa
     6361 ccatattttt aatatacaat ttcaaggtaa aagtttaaat caaatagata ttatatccaa
     6421 tctgctaaaa aatatcagct gaaaatttca tcttttcaac ttattacaaa agtatgccaa
     6481 agaatcctga taacatttta ataaaagcaa atggagataa agatgaaaat catcaagatc
     6541 agtacgatac taaatctctg catcacgcta ttcctgagag agttacccat tctatatctt
     6601 ttaagaaatt tatacagtaa atggacggat tttcgactga ttcgttgcat tctcttgtat
     6661 ataaattcat actggtacaa atagcaaaca tccgacaatc aaacaacacc atacaatcta
     6721 caaataacag tcaggcaaaa tacaccctgt tggtaacata caaactgtca aatgtgtgaa
     6781 taatataact atcatacacg aacatgtaag acttaatact ttaaactctc tcacctttat
     6841 ggtattatta aaacagctct tcataaacgc agagtttaga aaagggtgct gtaaatcaaa
     6901 ttttaaaacc aagaaaatga cagcaaaaaa ttacttacga atggcgtcac ttgctttatt
     6961 tattgcaatc gcctcgccgg ccagttcggc tgcaattgtt cctgaatcta tcacaccaac
     7021 agaagatgtc agaagtcagc aactgttgca acgtctcgaa gaaatcaaag gcctggacaa
     7081 atccgaaatg tcaagattag agaagaaaag cctccggaaa gaagtgaaag aaataaaaaa
     7141 agaaatgaaa gcgctgagcg gtggagttta cttgtcagtt ggggcaatca ttattatcat
     7201 tttattgctc attcttattt tataagacct tcctttttgc aaagtttagc agaaaatgaa
     7261 cagagcaatc atctttcaac agaccattaa tcattaacgt ttacctggtg tttctatgaa
     7321 atcgctatta tttgttgtaa gcctcttttc ctttattatg tacagggctt ccaggtcaga
     7381 tataccttta aagggaacct gggatggcag ttacaggaca atagagaaaa agatggacct
     7441 caggatagtt ttcggaccag gtaatgaagt tcaattatat agcactgaaa ttgataataa
     7501 gggaaaagct gtaggaacct atgccgttaa aagaaatgag atagcgatca cctgtatttg
     7561 gcctgacagt gacagtattc cattcataat gaaaggcaga ttaaactcaa atcagaattt
     7621 tgtagatgga aactgggaat cagccgataa aacagatggc aatttttacc tggcaaaaac
     7681 agtatcaaaa taattttaag caggaaggtt tggatatttc tcactgtcag acacggaccc
     7741 cggtatcctc cggggtttct tattatttac ctggtaatag tagttgtctg tttataccaa
     7801 atatgtgaat catgtaactt atttataagg ttgtgtaaca accagcagta aaaaatctcc
     7861 cacctttaat acatcattta aaaactctat tatgccacta cttaccattt tacttgtgct
     7921 gattattgcc ggtgttattc tttggctggt gaatacctat ataccaatgg atagaaagat
     7981 caaaaatatc ctgaatattg ttgtagtcat catcgttgtc atctggcttt tacaggcatt
     8041 tggcctgtta gactccctga aaagtataaa agtataaaaa gaaacagcct gcttacaatt
     8101 cgactatgta caatattgag ttgtagtagg gttatgggac aactatcaaa atagattcta
     8161 tcctgatcta aaaataatat gaaactattt aaactactta gtttcacagt tgccctgttt
     8221 cttacaacga tcctgctaat cgtggctttt attcttctgt atagtaaaac cggttgaata
     8281 tcttttcaaa ccaaaaccag gttacataag agaacagaac gctgccttaa atcacaggag
     8341 gcaaacagcc agtaattaca gtgcatacca ttcttatagt aagaaatttt tatactgtac
     8401 tctaagagga aaattatttt cataaaatga aaatagcttt tgaacatacg catgtttcaa
     8461 ttgaaatcat attggattgg cagtatcttt taaaatagta aaaaataaat atgaatatca
     8521 aaagaatctt tggggctttg cttaccattc tgggaacagc aggtctcatt tatggtgctg
     8581 tactcttcat taatacccct gggggtaccc gggatataaa agctcttgcc atatacggaa
     8641 tactaggtct tatattcttc attgcaggaa ttaaccttat ccgtaccaca aaagatgagt
     8701 catgatatgt gaatgctgta acttattctc agggttgtat aataagaacc gttacataaa
     8761 agctcatctt tacaagcaat atttattgaa tcatttataa aacaaataaa ttatgaaatc
     8821 aggtaaagta ttattaggtg ttttggcagg acttgccact ggtgctgcgt taggaatttt
     8881 atttgccccc gacaaaggtt cagcaacccg gaaaaaaata tcaaaaaagg gcgaagaata
     8941 tgctgataaa ttcggcgaaa agtacaatga attcattact ggaattaaaa ataaattcgt
     9001 gactgccaaa gaagaagctg tgcaactggc agagaatgga aaagcaaagg ctgaggaaat
     9061 tgcagctgca gcaaaaaaca ggatgtgata gtgcaaaatt aaaaaccgca aatctggccc
     9121 cgattatccc tgtatcctgg aataacaagc ctaaacagaa aatgatgaac gaaagcattg
     9181 taacaccgat agaagagctc attgaaaaag ccggtgagta tggaaaaaca tcaatagaat
     9241 tactaaagct gaaagcaata gataaatctg tcagtgtgat ttcatctctc gctgtaaaaa
     9301 cagttttact tattgttgtt gcaatgttga ttttaagtat cagcatcggt gtagcattgt
     9361 ggacgggtga attgcttggc aggccttatt acggcttttt tgtaatggct gggttctatg
     9421 ccctggccac cattgtactt tttctattcc gtgatcaatg gatcagaaag ccagtcaatg
     9481 agtcaatgat aaaaaaaata cttcaataag gtttccgatg aaaacaatca attcaacaag
     9541 tgaacttacc gaagccattt tattgttgga aaccaggcag gcggaccagg gaattcagtt
     9601 gagagaacaa tttaatctta cctatgagag cctaaaaccc cttaatctta ttaaaagtac
     9661 attcagagag gctgcttctt cccgggagct gaaggacaat atcattaata caggtacagg
     9721 tcttgtagcg ggctatcttt caaagaaatt atttgaaggc gtatcgcaca gtccattaaa
     9781 aaaaattctt ggctccgctt tgatgttcgg gataacaaat atggtgagca ggaacccgga
     9841 aactataaaa tcactgggac aaaaatttct gaatggcatc agtttcaaac cagttgataa
     9901 aaatgtaaac agaaatgata agaatgaaat tttttaaata gttacaattt ttcagataaa
     9961 ttgcatctca taaaagaata gtattccgcc accttatgta tcttacaatt attcttcctt
    10021 tgaaacctgc aagtttaatt tcaattcatt aatatccatc tcctgctcaa cttgcctgat
    10081 cactgcatca ctgaactccc cctctttgtg aagttttatc aacaatgccc tttgaaattt
    10141 gctgatttct aattgtgcac ttaacaacac accctgttgc tttgccatta cctcactgtc
    10201 atttcgtttg gcttttttat gacgatttat ttctttggta agttcattga tcagtacttc
    10261 atatttcatt ttcagttgtt gctccgattt attatccaat ggtaccggga aatcctgctc
    10321 aataaaatgc agggtgctgt ttgcaagata taattgaagt tctttctctt cctcctctgt
    10381 attgtcctgg ggtttcatct tcaaccagcg tatgagcagc gggagtgaaa gaccctgtac
    10441 aacaagggtt acaaaaatga caacgaaggc aagaaatatg atcgaatgtc ttttgggaaa
    10501 tgcggtgccg tcttccaaaa ccaatggtaa tgccagtgcc gctgccaaag atataacccc
    10561 cctggttcct gtccaggcta ctaccagcac atttttccag gtactgtccg aatcatcttt
    10621 gccagctatt acaccagttt tctttttcct cttttgaaaa taattttgtg accaggcacc
    10681 tgcaaaaacc catacaatcc ggactatgat cgtcacaagg ctgataagca atccataacc
    10741 cagtatctgt gagaacgggt aattacccag ccccttaata atagaaggca attgaaggcc
    10801 aataagtatg aaaacaaatc catgtaataa gaatatcaat gtatcccaca caaccctggt
    10861 acgcattctt gcctggtaag aaaaaacctc aggtgctctc catgctataa ccagtcctgt
    10921 acttacgaca gccagtaccc cggacatatg gaactgctct gccgccaggt aagatacgaa
    10981 cggagataac agggtaagac ttgtttcaac tacaggatta ctatcaattt ttttatgggc
    11041 aaacacaaac aggtacccaa tcacaattcc gataataatt ccaacaccag ctaccagtaa
    11101 aaactgaaga ccggcctgcc agaatacaaa gcttcccgtt gttacagccg ttacggcata
    11161 gcggtaagca atcaatgcag aagcatcgtt gaccaggctt tcaccttcca aaatggtgat
    11221 caccttctta tttaacccca gccctttaat gataccagag gctgctactg catcaggcgg
    11281 ggaaacaaca gcacctagta caaaagcaag cggccagcta aaccccggga ttaaaaaatg
    11341 ggcggtaatg gcaacagcta cagttgtaaa aaaaaccaat gtaatggcta atgcagagat
    11401 aggccttatg gatgtcctga attcgtgcca ggatgttttt gaagcggcat catacaataa
    11461 tggcggaaga agtataacaa aaacaacctc cgggtccagt gccaggtcgg gtaactgcgg
    11521 aacaaatcca attattaatc cggcagccac caataaaacc ggaaacggaa gctttcgttt
    11581 attggcaatc gctgtcaaac tgatcaggat agccataata aaaacaacga ttttaaaatc
    11641 ttccatacaa ctcaagctac gaaaaagcta cagattaaat tgaaaatacc agtcaaaaac
    11701 aagaccgaat taaactgtat cgtaacaaca ggtatactat cagccaggct gttttgtgat
    11761 actgtatctg atccggacgg tatagccgga taaaaaaagg gtcaacaata agttgacccc
    11821 aggtatagaa aattgtaaaa ttatttatgc ccaaaaagat agaatactcc taattgaaaa
    11881 cccctgttag tagacttgac agtactgtta tcattaatat tactcaggcc tagattatat
    11941 cttgcatcga ttccaaaacc agtacccgga ttgacataac cggcaccgat acccaacgca
    12001 aagtcaaccg tttctaaatc atccttaata tctacactgt tgccattaac atcagctttt
    12061 gcactgacca ggaatccaac ctgaggtcct gcctgtaacc tgaatccgtt gtcaaacatg
    12121 tactgaacca ataccggcac attgatatat cccagtttat actttgtttc aacacttcca
    12181 acagaatatt ttgcaccctg tgcagagtaa actaactcag gttgcaacgc caattgtttt
    12241 gccaggtgta tatgacccag caaacccagg tgaatgccgg cctttgtatc gtactttgta
    12301 ccattatcat tattgatatt gtagagattt aatcctccct taactccaac atttatatgt
    12361 tgtgcattaa ccgtacccgt tatcaaaatt gcaatgacta ctgcgctaaa ataaaatttc
    12421 atacttattt gtttttatta ttaaatgcct gaaaaactga tttgtctctc agttgatttt
    12481 ctttaataa
//
LOCUS       DAORTI010000104        12431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5111_length_12431_cov_5.709276, whole genome shotgun sequence.
ACCESSION   DAORTI010000104 DAORTI010000000
VERSION     DAORTI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5111_length_12431_cov_5.709276"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLO70_12355"
     CDS             <1..734
                     /locus_tag="PLO70_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQZ75305.1"
                     /translation="DDIMDKAFLWRGMEIVHTKFGDNTALLAGDVMLVKAYEYLSKID
                     TACLYPILYLFNQTAREVCEGQQWDMDFEKKETVNLDEYLRMIELKTSVLLAASLKMG
                     AILGGSGERNQNLLYEFGRKLGIAFQVQDDYLDAFGDPQKFGKQVGGDILANKKTFLL
                     IHAMESASVGQQKELSKLLNGNEAGKVENVLQIFRDCKVGEWALQLKNKYLDEAFAHL
                     EDIAVLSKRKEPLKELAHFLVQRQH"
     gene            805..2562
                     /locus_tag="PLO70_12360"
     CDS             805..2562
                     /locus_tag="PLO70_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQZ75306.1"
                     /translation="MSNSLFRRKSIDKIVHDVDLPADDIHSHGLKRVLTVKDLTFFGI
                     AAIIGAGSFSSLGGAVFNGGPGVVILFVITGIACAFTAFCYSEFASRIPVAGSAYTYA
                     YASFGELFAWIIGWALIMEYSIGNIYVAFSWSDYFTSFLHKTNIHIPDYLTCSYIEAK
                     KAVAEGSSNLELSGAWNSAPKIGGMRMIFDLPAIIINLLITYLVYRGIKESRNFSNVM
                     VILKLAIVGLVVLVGGYLVFSNGLTFNWMPSNEEGIRSFMPNGFSGVMMAVSGVFFAY
                     IGFDAVSVLAEESKNPQRDLPRGMILSLVICTIVYILLTLVLTGAVHYKKFDGIGDPL
                     AFIFEKENLNIGWMQILVSIVAVVAMTSVLLVFQMGQPRIWMSMSRDGLMPPVFQKIH
                     SKFKTPSFATIVTGLVVGVPILFTDKTFVLDFTSIATLFAFVLVCGGVLLIPRKEKVA
                     GQFSLPYINGQFIFPLIIAGVFILFVLLGKGYLADMVNFDFSANEDYINGKSSFMDLA
                     TPKISLIIFWIICILLSILTVIKKYSLIPLMGVVTCLYLLTGMTKSNWVWFMSWLILG
                     VIIYFAYGYKKSKLAPPVV"
     gene            2683..3702
                     /locus_tag="PLO70_12365"
     CDS             2683..3702
                     /locus_tag="PLO70_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQZ75307.1"
                     /translation="MKYQIGDIVLLLHSNEEGRIVDLINDQMLMVDVKGITFPVYKDQ
                     VDFPYFKRFSEKKILSTKKEKKYIDDVRKEKPEMRPRTEDGVWLTFLPVMDTDEFGDV
                     VVEELKLHLVNHTRTAFNFIYKLHFFGVPDFELKNTIQPFEDFYLHDIEFASLNDSPS
                     FDFEFSLVQPARNKETHYETSVRLKPKQLFAKIEELKKKNLATFSQLLFENYPDKVHD
                     DKVEMGKLAAKGYKIYEASEARKHLEPARSVVDLHIEKLTDNWRRMSNYEIVSLQLKT
                     FDKYYDLALAHHQPSLIIIHGVGEGKLRDEIHDKLRLKREVKSFVNQYHPNFGYGATE
                     IFFQY"
     gene            3725..4079
                     /gene="rnpB"
                     /locus_tag="PLO70_12370"
     ncRNA           3725..4079
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLO70_12370"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4079..4759)
                     /locus_tag="PLO70_12375"
     CDS             complement(4079..4759)
                     /locus_tag="PLO70_12375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQZ75308.1"
                     /translation="MTISPVFLFLILLAPAIFSVKNSLTISFIISPDKNWDHYWNHIL
                     YWPLLLVIVTALLLLIWRIFDKEEPFYGLTKKNFKLKPYLLMLIIMIPFIAAASTQTD
                     FLITYPKLKAIAEVANEKKIDWWHQLLFELSYGSDFFTTELFFRGFLILAFIKWAGRD
                     TILPMAVFYCTIHFGKPLGECISSYFGGILLGVIVYNTRSIWGGLIVHLGIAWLMEVG
                     GYIGNMIK"
     gene            5091..6005
                     /locus_tag="PLO70_12380"
     CDS             5091..6005
                     /locus_tag="PLO70_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75309.1"
                     /translation="MKTLSLNRITVTAFAVLFSFFLISCQKENSLSTTADTLTEEEAA
                     NFSDESSQAEASFDDAEDVSMIAAEEEGDASMYGRSADASGRLLPAFAELRLRLGPCA
                     VITVTPNDSTYPKTITIDFGPNGCLCADGKYRRGAIVIHLTAPIRRPGAVMTITFVDF
                     YLNRAHIEGTKVLSNLSENGNIKFTVQVTGGKVTFPNGRGYKYDGIKYVKQIQGGNTR
                     PIRDDAYSIEGRSKTEFNGGLVVNLNTESPLIKKVVCPWISNGVLKIKINSRVLFLDY
                     GAPNNGNCDNKAMLTWNNGNNQRLITLP"
     gene            6067..6885
                     /locus_tag="PLO70_12385"
     CDS             6067..6885
                     /locus_tag="PLO70_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75310.1"
                     /translation="MRYIVFIVFTMFLSCQHASKNENLHKIVSGNWFILYPEEDLLNK
                     KQDSIYAVIQDSLTGLKCLKLIRFSENGVFNQIDSVNIKGNWTIKEENNLIVYDGGRG
                     FGSFKSVFSGFKDGVLRLTEVVNMGGQRLKLIWHLKKIDKGYATKLFDEEYNRWRRRP
                     DRIESDDELKERVFKMVEYYSTYFQLIADESSYFIPGRIILPVNFYRHGISLKKFDDQ
                     SKFASFFYTNEQAKMAYLFLEAAYDNAKLDIPEKKSYSAEYSLMLKEIAQNLEK"
     gene            complement(6919..7629)
                     /locus_tag="PLO70_12390"
     CDS             complement(6919..7629)
                     /locus_tag="PLO70_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75311.1"
                     /translation="MKKILFLLFTGSLFLASCKKDNFIPPIIEESCVLQTGNPAGRSY
                     TSDSLISFTCTAKHCGILPLSTKNYWIYEDSVFSDGVFQKVQYDTLRFHKTWKSLSDE
                     LVWWESNISIGLPDKLYANDSAFFKIEDRFFTPGIVDVKKDFSLFPGDSIRYLASFED
                     AAAMGRSLKMQETIKTPAGSFSDYVYFEKNARNYRKDQVYFKPGVGVVKYIQEKAPMG
                     SPHIMLQQVCTLISYHIE"
     gene            complement(7827..8663)
                     /locus_tag="PLO70_12395"
     CDS             complement(7827..8663)
                     /locus_tag="PLO70_12395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQZ75312.1"
                     /translation="MKKYFLPVTVVLSLLFVSCNNDDKKKETEMKEPKLKEENVTYSP
                     AGIKDSLVLDGYVAYDENIEGKRPAVLVVHEWWGLNDYVKMRVRELAKLGYIAMALDM
                     YGNGKRADNPTDAGNLAGPFYQNPQMAKERFDAALVKLKSYSQADASKVAAIGYCFGG
                     TQVLNMANLGEDLVAVVSFHGGLKVVTPDKNLLKAQVLVCHGAADPFVPQAEVDLFRK
                     QMDSIGAKYTFKAYEGATHAFSNPDATANGQKFNIPIAYNAAADTASWKDMKDFFGTI
                     FK"
     gene            8828..9920
                     /gene="prfB"
                     /locus_tag="PLO70_12400"
     CDS             join(8828..8887,8889..9920)
                     /gene="prfB"
                     /locus_tag="PLO70_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_124635252.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQZ75313.1"
                     /translation="MTIEKLNDMRSRIAGIRRFLDVDNRQYKIAEDKQLSLSPGFWDD
                     NKRATEILKNIKLNEYWIKLYETAETAVEDFAVLFDFWKAGDATEEETKEAYDKALLL
                     LDDAEFKATLNEPEDELPAVLQINSGAGGTESQDWAEMLARMYRMYGEKQGWKITELD
                     WQWGDGAGIKTCTLQFDGPFAYGFLKAESGVHRLVRISPFDSNARRHTSFVSVFVYPL
                     VDDTINIEINPADVKMETSRSGGAGGQNVNKVETKVQLTHIPTGIVVVCQQDRSQLGN
                     RELAMQMLKSRLYEEELRKRQAIKDATNSTKKKIEWGSQIRSYVFHPYKMIKDHRTDY
                     EVGNVGPVMDGELDGFIKAYLMSEKETSS"
     gene            10008..11639
                     /gene="pruA"
                     /locus_tag="PLO70_12405"
     CDS             10008..11639
                     /gene="pruA"
                     /locus_tag="PLO70_12405"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQZ75314.1"
                     /translation="MSIGTFAYPMPVNEPVLSYAPGSAEKKRLKEVLEELKGFEIDVP
                     MYIGSKEVRTGKKVAMHPPHEKKHTLGYFHAGEEKHVHLAIEAALAAKDSWANTSWEN
                     RANIFLKAADLLATKYRPYINGCTMLGQSKNAFQAEIDAACELIDFLRFNVHFLSEIY
                     KQQPISSPGMHNRMEYRPLEGFVLAITPFNFTAIGGNLPTSAALCGNVVVWKCANTQI
                     YAAQMTMRVLKEAGLPDGVINLIYVDGPTIGKVCFNHRDFAGVHFTGSTGVFNNMWET
                     IGKNMANYKSYPRIVGETGGKDFVIAHKTADPDVVVTALLRGAFEFQGQKCSAASRAY
                     IPSNMAEEVKKKLIAGVKSFKMGTTEDFSNFINAVIDEKSFNNIKRYIDNAKKDPKAE
                     IWVGGKCDSKEGWFIQPTIIEAKNPKYVTMCEEIFGPVLTVYIYPANSFEKTLELVDS
                     TSPYALTGSIIAQDRAAVELATHKLRNAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSILNLYRWLSARTIKETFNPPTDYGYPFMREA"
     gene            11720..12322
                     /locus_tag="PLO70_12410"
     CDS             11720..12322
                     /locus_tag="PLO70_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQZ75315.1"
                     /translation="MKKIILSVVMLSATFFVSQAQVQYGLKAGANFYTFGGDEVENED
                     LNSKFGLHIGGLVNFSVSEKFKVQPEIVFSIQGSKQTDGTDELNWNFNYLNIPIMAQF
                     YVSNGFFLETGPQIGFNLKAEIKDEESGTTFDLDDEIKSTDISWGLGAGYKTASGFGF
                     GARYNLGLSSIAEESDVDIKNRGFQVGVFYLFGGKKAKKD"
BASE COUNT         3850 a         2179 c         2704 g         3698 t
ORIGIN      
        1 acgatgatat aatggacaag gcctttttat ggaggggaat ggaaatagtt catacaaagt
       61 ttggtgataa tacggccctg cttgcgggtg acgtgatgct ggtaaaagct tatgaatatc
      121 ttagtaagat tgataccgcc tgcctgtatc ctatcctcta tttatttaat caaactgcca
      181 gagaagtatg tgaaggtcag cagtgggata tggattttga aaaaaaagaa acggttaacc
      241 tggatgaata tctccggatg attgaactta aaacatctgt tttattggcc gccagtttaa
      301 agatgggagc catactgggt ggttcaggcg aaagaaatca aaaccttttg tatgagtttg
      361 gaagaaaatt aggtatagct ttccaggtgc aggatgatta tctggatgca tttggtgacc
      421 ctcaaaaatt tggcaaacag gtagggggtg atattctggc caataagaaa acattcctgc
      481 ttatccatgc aatggagtca gcctccgttg gtcagcaaaa agaattaagt aagcttctta
      541 atgggaatga ggcaggaaaa gttgagaatg tattgcagat attcagagat tgtaaagtgg
      601 gtgaatgggc tttacagtta aaaaacaaat accttgacga agcctttgcg catctagagg
      661 atattgccgt attatcaaag agaaaagagc ccttaaaaga gctggcgcat ttccttgttc
      721 agcggcaaca ttaacatatg tggaaactta gtttccggct accaattttt tccctatttt
      781 gacaaattaa aaaccaactg gctgatgtca aattcattat tccgtcgtaa atctattgat
      841 aagattgtgc atgatgttga tcttcctgca gatgatattc atagtcatgg attgaaacgg
      901 gtgttaaccg ttaaggatct tacattcttt ggtattgccg ccattatcgg agccggcagt
      961 ttcagtagcc tgggaggtgc agtatttaat ggtgggccgg gtgttgttat tttgtttgta
     1021 ataacaggga ttgcctgtgc ttttacagct ttttgttatt cagaatttgc aagccggatc
     1081 cctgtagctg gaagcgctta tacctatgcc tatgcaagtt ttggggaatt atttgcctgg
     1141 ataattggct gggcgctgat catggaatat tctatcggta acatttatgt agcatttagc
     1201 tggagtgatt acttcacctc ttttttgcat aaaaccaata ttcatatacc cgattatctt
     1261 acctgcagtt acattgaggc aaagaaagcc gttgcagaag ggtcttcaaa cctggaatta
     1321 tcaggtgcct ggaattcagc tccgaaaatt ggggggatga ggatgatatt tgatctgcct
     1381 gctatcataa taaatctcct gattacatat ctcgtttaca gaggaatcaa agaaagccgg
     1441 aatttcagca atgtgatggt gatcttaaaa ttggccattg ttggtttggt ggtgctggtg
     1501 ggtggatacc ttgttttttc aaatggactg acttttaact ggatgccatc caatgaagag
     1561 ggaatacgtt catttatgcc taatggattt agcggggtga tgatggcagt aagcggagta
     1621 ttctttgcat atattgggtt tgatgccgta agtgtattgg ccgaagaaag taaaaacccg
     1681 caaagagatt taccgagggg catgattctt tcgctggtta tttgcactat tgtctatatc
     1741 ctccttacgc tggtacttac aggtgctgtt cattataaaa aatttgatgg cattggagac
     1801 ccgctggctt ttatctttga aaaggaaaat ctcaatattg gctggatgca gattttagtt
     1861 tctatcgttg ctgtagtggc catgaccagt gttctccttg tttttcaaat gggacaaccc
     1921 cgtatctgga tgagcatgag cagggatgga ctgatgcccc ccgtatttca gaagatacat
     1981 tctaagttca aaacaccttc ctttgcaacg attgttacag gattggtagt aggtgtaccc
     2041 attttattta cggacaaaac ttttgtactt gattttacca gtattgccac gttgtttgct
     2101 tttgtactgg tctgcggcgg cgtattgtta ataccccgga aagaaaaggt agcaggccag
     2161 ttctcacttc cttatatcaa tgggcagttt atatttcctt taatcattgc cggagttttc
     2221 attttatttg tcctgcttgg aaaaggatac cttgctgata tggtcaactt tgatttttct
     2281 gccaatgaag attatattaa cggaaagtca agttttatgg atctggccac ccctaaaata
     2341 tctttgatta ttttttggat tatttgtatt cttctctcta ttcttacagt tattaaaaag
     2401 tactcactta taccgttaat gggagtggtt acctgtttgt atttactgac aggaatgacc
     2461 aagagtaact gggtctggtt catgagttgg ctgatactag gagtaattat ctattttgca
     2521 tatgggtata aaaaaagtaa actggctcca ccagtagttt agttaaaaaa atagttttat
     2581 agaattgagt tgtacatgga tatgtgcaac tctttttttt gagagctatt gcagccagat
     2641 tttgtaaatt tgtagttccc tttataaata aaggaccagc gtatgaagta ccagataggt
     2701 gatattgtgc tgcttcttca ctcaaatgaa gaaggaagga ttgtcgattt aatcaatgac
     2761 cagatgctga tggtggatgt gaagggaata acctttcctg tatataaaga ccaggttgat
     2821 tttccttatt ttaaaagatt ttcagagaaa aagatattga gtactaaaaa agaaaaaaaa
     2881 tacattgatg atgtacgaaa agaaaaacca gaaatgagac ccagaactga agacggggtc
     2941 tggcttactt ttttacccgt aatggacact gatgaatttg gagatgttgt ggtggaggag
     3001 ttgaaactgc atttagttaa ccatacccgg acagcattta attttattta taagctgcat
     3061 ttttttggtg tacctgattt tgaactgaag aatactattc agccatttga agatttctac
     3121 ctgcatgata ttgaatttgc cagcctgaat gacagccctt cctttgactt tgagttttct
     3181 ttagtacagc ctgccagaaa caaggaaact cattacgaaa catcggtcag gttaaagccc
     3241 aaacaactct ttgctaaaat tgaagaatta aagaagaaaa atcttgctac tttttcacag
     3301 ctccttttcg aaaattatcc ggataaggtg catgatgaca aagtggagat gggtaaacta
     3361 gctgcaaaag gatataagat ttatgaggct tcagaggccc ggaaacattt ggagcctgcc
     3421 cgcagtgtag tggatcttca tattgaaaaa ttgacagata actggcgacg gatgagtaat
     3481 tatgagattg taagcctcca gttaaagact tttgataagt actatgatct tgctttagcg
     3541 catcaccagc cttccctgat catcattcat ggggtaggag aaggaaagct gagggatgag
     3601 atacatgata aactccgact caaacgggag gtgaaatcat ttgttaatca gtatcacccc
     3661 aattttggat atggggctac tgaaatattc tttcaatact gattttcatg gttatttttg
     3721 taatactaca agccaggcta tcgactcggc tattttagtt gggtaggaaa gtccggacaa
     3781 cacagagcaa tgcaccggtg aagagccggc tgactggtaa cagtcaagag acagtgccac
     3841 agaaaataac tatcccggtg caaatcggga caaaggtgaa aacgtagggt aagagcctac
     3901 ggcttttggt ggtaacacta attgcgggta aaccttgcat gttgaaatgc cacatatagt
     3961 accgttttga ggactgctcg tccaaggtac agggtaggca gattgatccc ggtgggtaac
     4021 tgccgggaca gataaatgat agcccaagac agaatccggc ttaccggctt gtagtttttt
     4081 attttatcat atttccgata tagccgccaa cttccattaa ccacgcaatt cccaggtgta
     4141 ctattaaccc tccccagata gaacgggtat tatacacaat taccccaagg agtattcctc
     4201 caaaataaga actgatacat tctcccaaag gtttgccaaa atgaatggta caataaaaaa
     4261 cagccatcgg caggattgta tccctgcctg cccattttat aaaggcgagt atcaaaaaac
     4321 cacgaaagaa caattcagtt gtaaaaaaat cacttccgta ggatagttca aaaagtaatt
     4381 gatgccacca atcgattttt ttttcattcg ctacttctgc aattgcttta agttttggat
     4441 aagtaataag aaaatcagtt tgggtggatg ctgcggcaat gaatggaatc attataatca
     4501 acattaaaag atatggctta agcttgaaat tcttttttgt taatccataa aatggttctt
     4561 ctttatcaaa gattctccat atgagcaaca gtaaagctgt tacaataacc agtaaaagag
     4621 gccagtaaag gatatggttc cagtaatgat cccagttttt atccggggaa atgataaaac
     4681 tgattgtaag actgttctta acagaaaaga tcgcaggggc cagcagaata agaaatagaa
     4741 aaactggaga tatcgtcatc tttccttttc tatatatgtg aagcaccaaa tggggaaggg
     4801 caaaggcaac cccgaagact gcataccata gaataagttt aacggcaagt gaacttttat
     4861 tttggataaa ttcattgatc ccaaaatagt aattcgagac aacaagggtg gctgtaaaaa
     4921 tggaaaccag tattagaacc tgtttattgg tttcgtagaa ataccccttt aaatagttaa
     4981 tgataacctg catttaaaga gtaaaataaa ataatttatc cttctttaaa ctttcttgct
     5041 ctgtttagtt ccaaagtttc gttaataatt tataaacaaa aagtatagcc atgaaaactt
     5101 tatcactaaa ccggattaca gttactgcgt ttgcagtatt attctcattt tttctgatat
     5161 cctgtcagaa agaaaacagt ctctctacca ccgctgatac attaacagaa gaagaagcag
     5221 caaatttttc tgatgagagc agccaggcgg aagccagctt cgatgatgca gaagatgtaa
     5281 gtatgatagc cgcagaagaa gaaggcgatg caagtatgta tggccgttct gctgatgcca
     5341 gtggtcgttt gttgcccgct tttgcagaac tacgtttacg cctcggacct tgtgcagtca
     5401 ttacggttac accgaatgac agcacctatc caaaaaccat taccattgat ttcgggccta
     5461 atggttgcct ctgtgctgat ggtaaatacc ggagaggagc gattgttatt cacctgacag
     5521 cccctatccg aagaccggga gctgtaatga caattacctt tgttgatttc tacctgaacc
     5581 gtgctcatat agagggtaca aaagtactca gtaacctgag tgagaatggg aatattaaat
     5641 ttactgtaca ggttacaggc gggaaagtaa cttttccaaa cggaagagga tacaagtatg
     5701 atggtatcaa gtatgtgaaa caaattcagg gaggtaacac ccgtccgatc cgcgacgatg
     5761 cgtatagtat tgaaggaaga tcaaaaacag aattcaatgg tggcctggta gtcaacctga
     5821 atacagaatc tccgttgatc aagaaagttg tttgtccctg gatcagcaac ggcgtattga
     5881 aaataaagat aaacagcagg gtgttgttcc ttgactatgg tgctccaaat aatggtaact
     5941 gtgataacaa ggcgatgctt acctggaata atggcaataa tcaaagactg ataaccttac
     6001 cttaatttct atgaactggt ttgtaaagtc cttctgcttt gcagagggac tttttatatt
     6061 tgcaacatgc gttatatagt atttattgtg ttcaccatgt tcctttcttg tcagcatgct
     6121 tcaaaaaatg agaacctgca taaaatagta agtggtaatt ggtttatatt atatccggag
     6181 gaagatcttt tgaataaaaa gcaggatagt atttatgcag tcatccagga ttcattaacc
     6241 ggtcttaaat gtttaaaatt gatccgtttt tcagaaaatg gcgtctttaa ccagatagac
     6301 agtgttaata taaaaggcaa ctggactatc aaagaagaaa ataacctcat tgtttatgat
     6361 ggtggaaggg gctttgggag ttttaaatct gttttctctg gttttaaaga tggggtgtta
     6421 cgattaacag aagttgtcaa tatgggggga caacgtttga aattgatctg gcacctgaag
     6481 aagattgata aaggttatgc aacaaaactg tttgatgaag agtataatag atggaggagg
     6541 aggccagatc gcattgaatc ggatgatgaa ttaaaagaac gggtattcaa aatggtggaa
     6601 tattactcaa cgtatttcca attaattgct gatgaatctt cttatttcat tccgggaagg
     6661 ataatactgc cggttaattt ttatcggcat ggtataagcc tgaaaaagtt tgatgaccaa
     6721 agtaagtttg catctttttt ttatacaaat gagcaggcca aaatggctta tcttttcctg
     6781 gaggctgctt atgataatgc caaacttgat attcctgaaa agaaaagtta cagtgcagaa
     6841 tattcattga tgttaaaaga gattgcccaa aacctggaaa aataattatt gctgctaaag
     6901 aagtgcttct ccgtttattt actcaatatg ataacttatt aatgtgcaca cctgttgcag
     6961 cataatatgc ggagaaccca taggagcttt ttcctggata tatttaacaa caccgacacc
     7021 aggcttgaaa taaacctggt ctttacggta gttccgggca tttttttcga agtaaacata
     7081 atcgctgaat gaaccagcag gtgttttaat agtttcctgc attttaagtg aacgacccat
     7141 cgcggctgca tcttcaaaac tggcaagata tcggatggaa tcaccgggga aaaggctaaa
     7201 atctttcttt acatcaacaa ttccgggtgt gaagaatcga tcttcaattt tgaagaaggc
     7261 ggaatcattg gcatataatt tatccggaag accaattgaa atattgcttt cccaccaaac
     7321 gagttcatca gaaagggatt tccaggtttt gtgaaaccga agggtgtcat actgaacctt
     7381 ttgaaagacc ccatcactaa aaaccgagtc ttcatagatc caatagttct ttgtactcag
     7441 gggtaaaata ccacaatgtt tagcggtaca tgtaaatgaa atcaacgaat ctgaagtgta
     7501 agaacgtcct gcagggttac cggtctgtaa tacacaggat tcttctatta tcgggggaat
     7561 aaaattgtcc ttcttacatg aagccaggaa aagactacca gtaaaaagaa gaaaaagtat
     7621 ttttttcaaa gggattcgtt tttagtatcc taaagggctg acaaaataga aactactttt
     7681 tgtatttcat agctttacta agtaattttt cgaatttggc gcaccgtatt ttatgcaatt
     7741 ttgctgatta taaagctttt ataataatta ttttaaaata aaaaagcccg gaaatttccg
     7801 ggctttcaaa atcgttagta aaagtactat ttaaagatag ttccgaaaaa gtctttcata
     7861 tccttccagg aggcagtgtc agctgcggca ttataggcaa tcgggatatt aaacttttgc
     7921 ccgttggcag ttgcatcagg attactaaag gcatgggtgg ccccctcgta ggctttaaat
     7981 gtgtatttcg ccccgataga gtccatttgc tttctgaaaa gatcaacttc tgcctgtggc
     8041 acaaatgggt cagccgcacc atggcagact aatacctgtg ctttaaggag atttttatcg
     8101 ggtgttacaa ctttcaaccc tccatggaaa cttaccacag ctacaagatc ttctcctaaa
     8161 ttggccatat ttaaaacctg ggtaccacca aaacaataac caatcgctgc aactttactt
     8221 gcatctgcct ggctgtatga ttttaattta accagtgcag catcaaacct ttctttggcc
     8281 atttgcggat tctggtaaaa aggacctgca agattacctg catcagtggg attatcagct
     8341 cttttcccat ttccatacat atctagcgcc attgcgatat agccaagttt ggcaagttcc
     8401 cttactctca tttttacata atcattcaat ccccaccact catgcacaac cagcacagcc
     8461 ggacgcttgc cttcaatatt ttcatcatag gccacataac catccagcac aaggctgtct
     8521 ttgatgccgg ccggtgaata agtgacattt tcttctttta atttgggttc tttcatttca
     8581 gtctcttttt ttttatcgtc gttattgcag gaaacaaata atagtgagag cactactgtt
     8641 actggtaaaa aatacttttt cattgcgtta aaatttttgt tcaaaaaaga tagcaacaat
     8701 gaagttcagg aaaagtttca gcataaatag gaaaaaaacc gattaaatta aaggcttatt
     8761 tccagggtga ttgataggcg tagttctata attattcagc taattgcctt tcccttaact
     8821 ttgcgtcatg acaatcgaaa aattaaatga tatgcggtcc cgcattgccg ggataaggag
     8881 gtttctttga cgtcgataac cgacaatata agatagcaga agacaagcaa ctttccctca
     8941 gcccgggttt ttgggatgat aataaaaggg ctactgaaat tcttaagaat atcaagctta
     9001 acgaatattg gataaaactc tatgagaccg ctgaaacggc cgtggaagat tttgccgtac
     9061 tattcgattt ttggaaagct ggtgatgcta ctgaagaaga aacaaaagaa gcctacgata
     9121 aagcattgct tttgctggat gatgctgaat ttaaagccac ccttaatgaa ccagaggatg
     9181 aattgccggc agttttgcag ataaactccg gggcaggggg caccgaaagc caggactggg
     9241 cagaaatgct tgcccgtatg taccgcatgt atggtgaaaa gcaaggctgg aaaataacag
     9301 aactggactg gcaatggggg gatggggctg gtattaaaac ctgtacctta caatttgacg
     9361 ggccctttgc ttatggtttc ttaaaagcag aaagtggtgt gcacaggctg gtacgaattt
     9421 ccccttttga cagcaatgca agaaggcata cttctttcgt ttctgtattt gtttatccac
     9481 tggtagacga tactatcaat atcgaaataa atcctgcaga tgtaaagatg gaaacgtcgc
     9541 gaagcggtgg tgccggcgga cagaatgtaa acaaagtaga aacgaaagta cagttaacac
     9601 atattcccac aggtattgta gttgtctgcc agcaggacag gagccagtta ggcaacaggg
     9661 aactggctat gcaaatgttg aaaagccgct tgtatgaaga agaactgcgt aaaaggcagg
     9721 caataaaaga tgctactaat tctaccaaga aaaaaatcga atggggtagc cagataagaa
     9781 gttatgtttt ccatccctat aaaatgatca aggatcaccg tactgattac gaagtgggca
     9841 acgtgggacc ggtaatggac ggagaactgg acggttttat taaagcatac ctgatgagtg
     9901 aaaaggaaac cagttcatag tttgctttac tattaatgcc ggaaacccgg aaatattaaa
     9961 acaagggtac aatttgaatt aaccattata actaaaaaaa tattgttatg agtatcggaa
    10021 cttttgctta cccaatgcct gttaatgagc ctgtattaag ctatgcacct ggttcagctg
    10081 aaaagaaaag attaaaagaa gtactggaag aattaaaggg ctttgagatt gatgtgccga
    10141 tgtatatcgg aagcaaagaa gtaagaaccg ggaaaaaagt agccatgcat ccgccgcatg
    10201 aaaaaaagca tacacttggc tattttcatg ccggagaaga gaagcatgtc cacctggcca
    10261 tcgaagcagc attagcagct aaagatagct gggccaatac aagttgggag aacagggcaa
    10321 atattttttt gaaagctgct gatttgttag caacaaaata ccgcccctat attaatggct
    10381 gtaccatgct ggggcaaagt aaaaatgctt tccaggcaga gatcgacgct gcctgtgagt
    10441 tgatcgattt tttacgtttc aatgtccatt ttctttctga aatatataaa caacaaccta
    10501 tcagcagccc gggaatgcat aatagaatgg agtatcgtcc cctggaaggt tttgtattgg
    10561 caattactcc tttcaacttt actgctatcg ggggtaacct tccgacatca gcagccttgt
    10621 gtggcaatgt ggtggtttgg aaatgtgcca atacacagat ttatgcagca caaatgacaa
    10681 tgcgggtatt aaaagaagcc ggcttacctg atggagttat caatttgatt tatgtagatg
    10741 gccccaccat tggtaaggtt tgtttcaatc accgggattt tgccggtgtt cattttaccg
    10801 gatctaccgg agtgtttaat aacatgtggg aaacgatcgg taagaatatg gctaattaca
    10861 aatcctatcc cagaattgta ggagagacgg gtggtaagga ttttgttatt gcccataaaa
    10921 ccgcagatcc tgatgtagtg gttacagcat tgcttcgtgg tgcgttcgag ttccagggac
    10981 aaaaatgttc tgctgcttct agagcttata tcccttctaa tatggcagaa gaagtgaaga
    11041 aaaaattaat cgccggtgtg aagagtttta aaatgggaac aacggaagat ttttcaaatt
    11101 ttattaatgc tgtgattgac gaaaagagtt ttaataatat taaaagatac atcgacaatg
    11161 ccaaaaaaga tcccaaagca gagatatggg tgggcggaaa atgtgatagt aaagaaggtt
    11221 ggtttattca gccaactatc atcgaggcga agaacccaaa gtatgtgacg atgtgcgaag
    11281 agatatttgg cccggttttg acagtttata tttatccggc aaatagtttt gaaaaaacac
    11341 tggaactggt tgattctacc tctccatatg cgttaaccgg ttcaatcatt gcccaggaca
    11401 gggcagcagt ggaattggcg acccataaac tacggaatgc ggccggaaat ttttatatca
    11461 atgacaaacc aaccggtgca gtagtagggc aacaaccctt tggtggtgcg aggggttctg
    11521 gaacaaatga caaggccggg tcgatattaa atttatatcg ctggttaagt gccaggacga
    11581 tcaaagaaac atttaatcct ccaacagatt acggttatcc gtttatgaga gaggcttaaa
    11641 aagagttcaa taaaagcttg caatttcctt gttggggata tacctttgtt gttggatatt
    11701 ttaaccaaaa aacaatttta tgaaaaaaat cattttatcg gtagttatgt tatcagctac
    11761 tttttttgtt tcacaggcac aggttcagta tggactaaaa gccggggcta atttttacac
    11821 attcgggggt gacgaagttg aaaatgaaga tttgaattca aagtttggcc ttcatatagg
    11881 cgggcttgtt aatttttctg tttcagaaaa atttaaagtt caacctgaaa tagtattttc
    11941 tatacagggt tctaagcaaa ctgacggaac tgatgagctt aactggaatt ttaattatct
    12001 caatattcca atcatggccc agttttatgt gtcaaacgga tttttcctgg agactggtcc
    12061 ccagatcggt ttcaatttaa aagctgaaat caaggatgaa gaaagtggta cgactttcga
    12121 tctggatgat gaaattaaga gtacagatat atcctggggt ttgggagctg gttataaaac
    12181 cgcttctggc tttggatttg gcgcccgtta caatcttggg ctttccagta tagctgaaga
    12241 aagtgatgta gatattaaga atcgtggatt ccaggttggt gttttttacc tgtttggtgg
    12301 aaaaaaagct aagaaagatt aattatttat ctgacttgat aatacaaagg ccggttttat
    12361 ccggcctttg tattttatac gaaataaaaa gaatttatta cgctcaaatt ctcgtaataa
    12421 attttctgtt t
//
LOCUS       DAORTI010000105        12403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5126_length_12403_cov_6.536929, whole genome shotgun sequence.
ACCESSION   DAORTI010000105 DAORTI010000000
VERSION     DAORTI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_12403_cov_6.536929"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..625)
                     /locus_tag="PLO70_12415"
     CDS             complement(326..625)
                     /locus_tag="PLO70_12415"
                     /inference="COORDINATES: protein motif:HMM:NF026918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 53 family protein"
                     /protein_id="HQZ75316.1"
                     /translation="MIEWLQNWYARQCDGKWEHEYGIKIDTLDNPGWSIVIDLKNTEL
                     ENLKIPYSLNERADEDWFGYSIENGVFKAAGGTHSLSIIIELFKGLVDSNVGNDI"
     gene            complement(644..1396)
                     /locus_tag="PLO70_12420"
                     /pseudo
     CDS             complement(644..1396)
                     /locus_tag="PLO70_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1707..1964)
                     /locus_tag="PLO70_12425"
     CDS             complement(1707..1964)
                     /locus_tag="PLO70_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75317.1"
                     /translation="MEYLGGTYISQVKAQDEYNAMRQWISNLNVHEIKGLTIRDKQKI
                     IKNDFEDEEAVLIKGIKNTWHFSIRTKKGIGFVNFVMTKKN"
     gene            complement(1989..2813)
                     /locus_tag="PLO70_12430"
                     /pseudo
     CDS             complement(1989..2813)
                     /locus_tag="PLO70_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3032..3487)
                     /locus_tag="PLO70_12435"
     CDS             complement(3032..3487)
                     /locus_tag="PLO70_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75318.1"
                     /translation="MIKHIALFILIAGVSCKTRVDNTLSEVKFHNKKITELIYKYIET
                     IKKDGRRFMTVICTKSNDTTSFIMIDSYPDNSKINLNGVTEINQVKIFFAGDCLESEF
                     LTLTTDNSEELDKEAEKINVQVNESVVKMREPTIWEFYFKGDSLFKYIP"
     gene            complement(3484..7635)
                     /locus_tag="PLO70_12440"
     CDS             complement(3484..7635)
                     /locus_tag="PLO70_12440"
                     /inference="COORDINATES: protein motif:HMM:NF039454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HQZ75319.1"
                     /translation="MKVQAIKIFLIVLLTVLYYHPIKAQIIPNGQGVPAVNYVPPLEQ
                     ANLGGRINYVRTWEPWKPITDAGSVSVQSIADVKVNTVYMDGQGRLAQTVSKQASPGG
                     NDLVAYKQFDRYGRETVQPLPYISFTTDGSFKSNPYAAQQYYYSTGAVNNNQYAGEQI
                     YFGRTALETSPLLRPDTVMAPGNSWAGSLKGVRYFYQVNTETDAVRIWTLGDAAGSVP
                     ATAGVYPEGRLYKNIVIDEDGKQVIEYKDLKGQIILKKVQESNTPGPAHSGWVCTYYI
                     YDDYGLLRCVIQPKGVQLCEVQGWSLSTEVLNEQCFRYEYDQRNRMIIKKVPGAGEVW
                     MLYDARDRLVMTQDANLRNSNPSKWHYTKYDEANRPISAGLLISSADRAAHQAAAYNS
                     TTYPELSYYSHQVFTTTIYDDNSEFSFDTQDIGKLDAGANPWPQAVVNSSIVYGKPVK
                     TTTATEGFLQPTNTYSFYDEKGRVIQVRVEDGRKRISTTTSRYDFSGKVIASYQRQYV
                     PDHVQGGPLTVTVLTKMLYDAGGRLLKTWKKINDGPTDKLIADNSYDELGQLKSKKLA
                     PAFNNNAGIETLEYSYNIRGWLKSINQQYATGSTNSNWFGQVLSYDHGFTFQQYNGNI
                     SGLQWRGKSDGEQRAFGFAYDNLNRFVSGDFTQLSGSGWNTNTGLDFTVNNMSYDANG
                     NIVTMNQKGWKLGGSLLIDQLTYSYETASNKLKQVLDGVNNLNSHLGDFKYDPLTKGS
                     TDYSYDNNGNLVRDNNKAIKDDNLQAIEYNHLNLPVLIRVKDKGTIAYRYDANGNKVS
                     KIVTEGGQKKVTNYFAGFVYEFTTTGAVEDGIDVLQFFGHEEGRIRPKGNEFVYDYFI
                     KDHLGNVRMVLAEEQQSDMYIAATMETATATQEETFYSNLPETRTDVPANYPPISGNQ
                     KVARLSGNEQSDPGGVKIGPAILLKVMAGDKFNVMVNSWWEAVTRPGTPTSPYNALLN
                     ALSGSIAAQNGNHGSNVELANSAEINATVGNYLNTQTNYNTTRPKAFLNWLLLDERFN
                     YVAGSSGFEQVGAADLLNTHTQTDKPVEKSGYLYIFVSNSTPNIDVFFDNLQVTHNRG
                     PLVEETHYYPFGLIMAGVSSKALNNTPENKYRYNGKEQQSKEFSDGSGLDWYDYGARM
                     YDAQIGRWNHIDPLSEKMRRYSPYNYAFDNPLRYIDPDGMKPTDDYQLGRNGIAVKIR
                     ETEDKTDRLYASKKNGEIDRAKSIEMSKGIANSAELTEAGLTVKVNNSKKSAQNLFEF
                     AANNTDVEIALIHIKNGDRYESYVSTSSSPEEVAINRTFLINETKDGKVVEEIDHSHV
                     TEGRISLWKPSGFDSNGKIIPAQDGDAAAAARNEQRVNPNIIHKVYIPDTGAYIQYNS
                     KRILK"
     gene            complement(7671..10538)
                     /locus_tag="PLO70_12445"
     CDS             complement(7671..10538)
                     /locus_tag="PLO70_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75320.1"
                     /translation="MNQILVSNGYGTTLRKAVFQYLDRHIPNPPFNEGFFLKKVILKD
                     PTTSSNEEQVFELHYVDEENALAGAAITNNIDHLGFANGASNSTLLPVVNTYVYNGFP
                     LFASYGTANREPNGVYAQKGMLQRIVYPTGGHDEFHYEPNMKTWWGNIEKKKIISARL
                     TETGGYPSQFYNTYFTVCEAQTGEFHLSTEWIGPLPVPGGDPAPKVAIATLYDNSTGL
                     VIEKRTAFGYSGFPNHEVWGDFHVPLSPGINYRFELEVRNGPNNKARADIVYDQLCGT
                     EWGQINEELCGVRVRKIVSYDNVSRKSHSKFYHYRSLTDTLNPSAQKLYYPQYVTAYS
                     NGLYCGSSNLEILCNGFLLSSNSATTLYDNEAGGVVYKWVIESDDSLFSNGGVEHTFI
                     KQGQFIGNQIIGETVPNPPVDPYNYMNGLESQTRFFNHNMEFVKKITNYYSTDSRIND
                     AELNNYIVRKRFSPMLAGTPPWDDYDFQHLDVTSYSIFTRWDHLDRTVTEEYDLVNNK
                     VITSEVLFTYGSANNILPTQTKIYDSEGTELRKEIKYPNDFSYLPVCSTMVAKNIVEP
                     VLEEKSYKETKLLSQTNTEYRDWFNNGSILAPEFIKISKGNGAQETRARYYQYDAKGN
                     PLEVAKENDIRLSYIWDYNATLPIAEVNNGSWGQGIAYCSFETTAKGGWTYSGTALTE
                     GTEPGGKKSYNLANANITYNGTLITGKKYVVSYWIKGGSVLVNGNAGIIQLSKNGWSL
                     YRGTIIYSGNGGVTVSGTGYIDELRLYPDGAFMKSFTYSPEVGITSAADINNIFQTYE
                     YDAFNRLLRIRDMDRNILKQFEYKFGTDLTGCNNSTPDWQMTGNYRCAKNNMVNNNNT
                     GIKEREEMDLNNCSESYGQTRWMSIGSSAECPVLSNCSGLDKRVVNGVCETGCKKLLK
                     SEYLGSLVWICTFRYEWTDGFKGPEFTESGPFHCLDVICDL"
     gene            complement(10728..11525)
                     /locus_tag="PLO70_12450"
     CDS             complement(10728..11525)
                     /locus_tag="PLO70_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75321.1"
                     /translation="MRFFTSIILLLFFYIEIEAQGSNNTFKLPEVLPPSPDLATLTKG
                     AELRSTPHTGGANSSIPIYELKLRNFSLPISVNYSSTGFKPEEIPSRVGLSWSLNAGG
                     AVTRIVKGKPDDYCSPPAQYLTESQVLAKNSNSFFFLEDLEDQNSNHDSQPDEYRYTV
                     NGLTGKFIIKRDGSVLQLPYNNVKIIVLKSQGAVTDIIITDGGGVKYTFGGGVQESTL
                     EHNLVYDLLQKQTITTAWMLTNVTLPNGDYIDFSYGSLTHYVNSGVI"
     gene            complement(11536..>12403)
                     /locus_tag="PLO70_12455"
     CDS             complement(11536..>12403)
                     /locus_tag="PLO70_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75322.1"
                     /translation="YYYAQQLNEYKEILKYPKKTEKKVLELLAKTKLFRDFMRKNSML
                     ASLFRMPGDPNDPAYVASLAGLQTRAQVNSLIQTQIAAGGPNAQAQFQQNLQAAQSQL
                     NELKDKILKAGGGSSNAEMPEGFKPNNQKTKSFLKRIELGTNIQSQKSTGFLPLTSDI
                     GVSAGYKLNDKSIVGLGASYKMGWGQNLRNIRISHQGLSARSFIDWKLKGSLWVSGGF
                     EMNYRSEFRNFNVLQNYSSWQQSGLIGISKTVPVKSKFFKKTSLKLLWDFLSYQQVPR
                     TQPVIFRIGYNF"
BASE COUNT         3286 a         2883 c         2178 g         4056 t
ORIGIN      
        1 gcatcgtata ttccaatttc aaaagcatct tctttttcaa taccattgta tttattttta
       61 ttctctggta catcattcaa tgccttacta ctgattcctg ccattattaa cccgaacgga
      121 tagtaatatc tggttcaaag agcttactgc aatgtacaaa atcagttgta aaatactaga
      181 ttagaaaacc tcgatttcaa gtcaagcctg ttacttcgta ttagcccaat gtcgcatgct
      241 ggtacataac accacacagc agacatgggc aggaacgatt gttgggggag aatctgggcg
      301 gaaaataata ttaactgtct caacatcata tatcattacc aacattagaa tcaactaaac
      361 ctttaaaaag ttcaattatt atgctaaggc tatgagttcc tcctgctgct ttaaaaacac
      421 cattttctat agagtatccg aaccaatcct catctgccct ttcatttaat gaataaggga
      481 tttttagatt ttccaattct gtgtttttta aatctatgac aatagaccaa ccagggttat
      541 ccaaggtatc tatctttatg ccatattcat gctcccactt gccatcacat tgacgagcat
      601 accaattttg tagccattct atcattacaa taattttgtc aaattacttc caaaactccc
      661 aaaatctttt attatttggt gtttcttgct tagtcggggc ttttactcct ccactctttt
      721 catttatatg aggggtctca acatttttcc ctgtcttctt atcaaaatgt ggcttgcctt
      781 ttgagccatc agactttccg ccatcaaatc tatccccttt atcgtaaccg ttcgggttaa
      841 aaggattttt ccaccattct tgatactgat aaacattacc ggcattatct ctctttaacg
      901 atacatgatc acctcctgca tcaggatcaa tatctaagtc gtcctgtttt ttaggagcgg
      961 ttttatctga attactactt gaagaattgt ctttcccatc gagccttcta tcctcgactg
     1021 attttattac ctcatctatg agtggctcag ccgtcttcac cactattgcg gccccgacag
     1081 ctgcaattac tgctttagca agaggtatta gaaataaaca gggacactta ccactcggat
     1141 cgttatatcg aaccggatta ttataaccaa attgaaaagg cgctaaggat ctttgatcca
     1201 cagtgtcact caaaggatca atttgcataa aacgacctat ctgttgatca tatgatcttg
     1261 cattaaaatc ataaaattca aggccgcttc catcattaaa ctctccgctt tgtaattcat
     1321 taccgttata acctttttta ttcttgggac taccaaaagc taaagccttt gaacttatcc
     1381 ctgccattat taacccgaac ggataataat acccggttca aagaactaga ttacaaggta
     1441 cacaaactgt tgtgaaatac tactcgaaaa atatccctgg ttaggcatta aacctcacaa
     1501 agacaaaaat gggttgaaat gaaattcagt aggaggtata attagcaaaa tatatttgag
     1561 tcgtagtctc agactgctgg caactttctg ataacttata atttgcttac tgattcaaga
     1621 acaataaaca caacggggct accgtgtatc tttcatttta tttattaatc attctaaggg
     1681 caaccaagaa gaagggattg gctttactaa ttcttttttg tcattacaaa gtttacaaat
     1741 cctataccct tttttgtcct aattgaaaaa tgccaagtat tttttatccc ttttatcaat
     1801 actgcttctt catcttcaaa atcatttttt attatttttt gtttatctct aatagtaagg
     1861 ccttttatct catgaacatt taagttgcta atccactgac gcattgcatt atactcatct
     1921 tgagccttaa cttgactaat ataagtaccc cctaaatatt ccattataaa agtatataat
     1981 tccatattct aattactccc tttcgctttt tgtgaatgat tttcaccagt ttcatccatc
     2041 tttttctttt gatgaatcat ttctgttttc gcttttgctt cagcggtctt atcttctttt
     2101 gttttattcg ctttactttt taaagtatta taattttctt tagccgcttc gtatttcttt
     2161 tgagcagctt ctcttgcctt ttgatttgca tgttgtgcat tttttccgcc ccctttccct
     2221 gatgagtttt gttgagcaaa tggaacaaca ggaggagata caggttgggt ttttacagag
     2281 actggagttg acccctttcc gacaattgga attaattcca taggaggagt tttcgcccta
     2341 gcaccaccaa gagtgaataa aggtccaact tttcctaccg taactgaatt attatataag
     2401 tctaatttgt caccgccaag cttatccctc attccaaaag gatcactgga aattagcccg
     2461 aaacttagtg tatttacagc tccatttaaa acaccgcttg cagaaatcaa aacatcttct
     2521 acaagattaa gtagcgcccc gcagcatccg tcagcacttt ccggttcatc acctaaaaaa
     2581 tccatgtaag taatcgggtt gtcataatta gaagcatacg gtgaccacat ttccatgttc
     2641 tcggtcttcg ggtctatctg ccaccatctt cccgtttgtc cgtctaactc tcttaactgt
     2701 gcatcgtata ttccaatttc aaaagcatct tctttttcaa taccattgta tttatactta
     2761 ttctctggta aattattcaa tgccttacta ctgatccccg ccattattaa cccgaacgga
     2821 tagtaatatc tggttcaaag agcttaatgc aatgtacaaa atcagttgta aaatactaga
     2881 ttagaaaacc tcgatttcaa gtcaagcctg ttacttcgta taagcccaat gtcggctgct
     2941 acacactatg ctgttcaccg ccagacatgg gcaggaaagt ttacgggtgg ggcggaggag
     3001 acgttggaag agaaaaaaat acaaaaactc gtcaggggat atatttaaat aaactatccc
     3061 ctttaaaata aaactcccaa atagtgggct ctctcatttt aacaacagac tcattaactt
     3121 gaacattaat tttttccgcc tctttatcta attcttctga attatctgtt gttaacgtaa
     3181 gaaattctga ctctaaacaa tctccagcaa aaaatatttt tacttgattt atttctgtga
     3241 ctccattcaa attaatttta ctgttatctg gataggaatc aatcataata aagcttgttg
     3301 tgtcattgct tttagtgcag ataactgtca tgaaccttcg tccgtctttt tttattgtct
     3361 ctatatattt ataaattaat tcagtaattt ttttgttatg aaatttgact tcactcaacg
     3421 tgttatcaac tcttgttttg catgaaactc ctgctattaa aataaaaagc gctatatgct
     3481 taatcatttt agtattcttt ttgagttata ttggatataa gctccagtat ctggtatgta
     3541 taccttatga ataatattgg gattaactcg ttgttcattt cttgcagctg ctgctgcatc
     3601 tccgtcttgc gcaggaatta tcttcccatt gctatcaaat ccagaaggtt tccataaact
     3661 tatccgccct tctgttacgt ggctatggtc aatttcctca accaccttac cgtctttggt
     3721 ttcatttatt aaaaatgttc tattgatggc aacctcctca gggctagatg atgttgacac
     3781 ataactttca tacctatccc cgttttttat atgaatcaac gcaatctcta cgtcagtgtt
     3841 attcgctgca aattcgaata ggttctgagc actttttttg ctattattaa ctttgactgt
     3901 caaacctgct tcagttaatt cagcactatt tgcaatcccc tttgacattt caatagattt
     3961 tgctctatca atttctccat ttttctttga agcatatagc ctgtcagtct tatcttcagt
     4021 ttctcttatt ttaacagcta taccatttct acccaactgg taatcatcag ttggcttcat
     4081 gccatccggg tcaatatatc ttaatggatt atcaaacgca taattgtacg gagagtaacg
     4141 ccgcattttt tcactcaacg ggtctatatg attccatctt cctatttggg catcatacat
     4201 tcttgcccca tagtcatacc aatctaaccc cgaaccatca gagaactctt ttgattgttg
     4261 ttctttgccg ttgtatctgt atttattttc tggagtgttg tttaaagcct tcgacgatac
     4321 tcccgccatt attaacccga acggataata atgcgtttcc tccaccagcg gcccacggtt
     4381 atgcgttacc tgtaaattgt caaagaacac atctatattg ggtgtactgt tacttacaaa
     4441 aatatacaaa taaccgcttt tttctacagg cttatccgtt tgtgtgtggg tatttaacag
     4501 atcagctgca cccacttgtt caaagccact gctgcccgct acatagttaa accgctcatc
     4561 cagcagcagc cagttcaaaa atgccttggg ccgtgtagta ttataatttg tctgggtgtt
     4621 tagataattg cccacagttg catttatctc tgcactattg gccagttcta cattgctgcc
     4681 atgattaccg ttctgtgctg cgatgctgcc actcagcgca ttcagcaggg cattataggg
     4741 gctggtaggg gtacccggtc tcgttacagc ctcccaccag ctgtttacca taacattaaa
     4801 cttatcgccg gccatcacct ttaataaaat ggccggtcca atttttacac cgccgggatc
     4861 ggattgttca ttaccactca gccgggccac tttttgatta ccacttatcg ggggataatt
     4921 agccggtaca tcggtccggg tttcgggcag attactatag aatgtttctt cctgggtggc
     4981 cgtggctgtt tccatcgtag ccgctatata catatcggat tgctgctctt cggccagcac
     5041 catccgtaca ttacccaaat gatctttgat aaaatagtca tagacaaact catttccttt
     5101 tggcctgatc cttccttctt catggccgaa aaattgcagc acatcaattc catcctctac
     5161 tgctccggtt gtggtaaact cgtaaacaaa accggcaaaa taattggtca ccttcttttg
     5221 gccgccctcc gtcactatct tgcttacttt attgccgttg gcatcatacc ggtaggcaat
     5281 ggttccctta tcttttaccc gtatcagcac cggcaggttc aggtggttgt actctatggc
     5341 ctgcaggtta tcatctttga tcgctttgtt attatccctt accaggttgc cattattgtc
     5401 atagctgtaa tctgtactac cctttgtgag cggatcgtat ttaaaatcac cgagatggct
     5461 atttaaatta tttaccccgt caagtacctg ctttagttta ttactggccg tctcataact
     5521 ataggtcagt tggtcaatga gtaatgatcc gccaagtttc cacccttttt gattcatcgt
     5581 aactatattc ccgttggcat cataactcat attgttgacg gtaaaatcta atccggtatt
     5641 ggtgttccat ccgctgcccg aaagttgggt aaaatcacca gatacaaacc ggttaaggtt
     5701 atcataggca aacccaaaag cccgctgctc accatcactt tttccccgcc attgcaggcc
     5761 gctgatattg ccattgtact gttggaaggt aaacccatga tcataactaa gcacctgtcc
     5821 aaaccaattg ctgttggtgc ttcctgtggc atattgctgg ttgatgcttt taagccagcc
     5881 ccggatatta taactgtatt ccagggtttc tattcctgca ttattattaa aggcaggcgc
     5941 cagtttcttt gatttgagct gacccagttc atcataactg ttgtctgcaa tcagtttatc
     6001 cgtagggcca tcatttattt ttttccaggt tttaagtaaa cggcctccgg catcgtaaag
     6061 cattttggta agcacagtca ccgtaagcgg gcctccctgt acatgatcgg ggacatattg
     6121 acgctggtag gaagctatta ctttcccgct gaaatcatat cggctcgtgg ttgtacttat
     6181 tcttttacgt ccatcttcga ccctcacctg tatcaccctg cctttttcat cataaaaact
     6241 ataggtgttc gttggctgca aaaatccctc tgtggctgtg gtagtcttta ccggcttgcc
     6301 atacactata gatgaattta ccaccgcctg cggccaggga ttggctcctg catccagctt
     6361 gcctatatcc tgtgtatcaa aagaaaactc tgagttatca tcatagatgg tagtcgtaaa
     6421 tacttgatgg gaataatagg agagttcagg ataggtagta ctattataag cagcagcctg
     6481 gtgtgctgcc ctgtcagcgc tgcttatcaa cagaccggca cttattggcc ggttggcttc
     6541 atcatacttt gtataatgcc actttgaagg attgctattc cgcaagtttg catcctgtgt
     6601 catcaccagc ctgtctcttg catcgtacaa catccatact tctccggctc ccggcacttt
     6661 tttaatgatc attctgttgc gctggtcata ttcataacgg aaacattgtt catttaatac
     6721 ttcagtgctt aacgaccatc cctgtacttc acataactgc acgcctttgg gctgtatcac
     6781 acagcgcaat aatccgtagt catcataaat ataataggta catacccatc cactgtgggc
     6841 cggaccaggt gtattgcttt cctgaacttt ctttaaaatt atctggccct ttaaatcctt
     6901 atactcaatt acctgcttac catcttcatc aataacaata ttcttatata acctgccttc
     6961 cgggtaaaca cctgctgttg caggaacact gccggcagca tcacccaagg tccatatccg
     7021 aaccgcatct gtttctgtat ttacctggta gaagtaacgt acgcccttca aactaccagc
     7081 ccagctgtta cccggagcca ttacagtatc aggcctcagt aagggtgatg tttccagtgc
     7141 tgttctgcca aaatatattt gttctccggc atactggtta ttgtttacag ccccggtact
     7201 ataataatat tgttgcgctg catatggatt ggacttgaaa ctaccatccg ttgtaaaaga
     7261 aatataggga agaggctgca cagtttcgcg gccgtagcga tcaaattgtt tataagcaac
     7321 aaggtcattg ccacccggtg aagcctgttt gcttactgtt tgcgctagcc tgccctgtcc
     7381 atccatataa acagtattta cttttacatc agcaatgctt tgcacggaga cactgcctgc
     7441 atctgtaata ggtttccatg gttcccatgt ccgcacataa ttgatgcggc ccccaaggtt
     7501 tgcctgttca agcggcggta cataattgac tgcaggcact ccttgcccgt tgggtattat
     7561 ctgcgctttg ataggatggt aataaagaac agttagcaga acgatcaaaa atatcttaat
     7621 ggcttgcact ttcatgtaaa ttcatttaat gctttattcg ttggctttat ttatagatca
     7681 cagattacgt caagacaatg gaagggacct gactctgtaa actcagggcc cttaaaacca
     7741 tctgtccact cataccggaa agtacaaatc cacaccaagc tacccaggta ttcagacttc
     7801 aataattttt tgcagccggt ctcacaaaca ccattcacta cccgtttatc aagcccgcta
     7861 caattactca atacaggaca ctctgctgaa ctgccgatac tcatccatct tgtctgccca
     7921 taactttcac tgcaattatt aaggtccatc tcttctcttt ccttaatacc tgtattgtta
     7981 ttgttaacca tattgttctt tgcacaacga taattgccgg tcatctgcca gtcaggggtt
     8041 gaattattgc agcctgtcag gtcagtcccg aacttatatt caaactgctt caggatattt
     8101 ctgtccatat ccctgatccg caataaccga ttaaaagcgt catattcata tgtttggaaa
     8161 atattgttta tatcggcagc agatgtaata cctacttcgg ggctgtaagt gaaggatttc
     8221 atgaaagccc catcagggta taatctaagc tcgtcaatat aaccagtgcc tgacacagtt
     8281 acccctccgt tgccagaata aatgattgtc ccccggtata aagaccagcc atttttactt
     8341 aactgtataa tgcccgcatt gccatttacc agaacactac ctcctttgat ccaataactc
     8401 actacatatt tcttgccggt aattaatgtt ccgttatagg taatatttgc gttagccaga
     8461 ttataagact ttttaccgcc gggctcggtt ccttctgtta atgctgtgcc actatatgtc
     8521 catccaccct tagcagttgt ttcaaatgag caataggcaa tcccctgtcc ccaactgcca
     8581 ttatttactt cagcaatggg cagggtagca ttataatccc atatatagga aaggcggata
     8641 tcgttttcct tcgcaacttc caaaggattg cctttggcat cgtactgata atatcttgcc
     8701 cttgtttcct gggcaccatt ccctttactg atctttataa attccggtgc cagtatactg
     8761 ccgttattaa accagtctct gtattccgta ttggtttggg ataacagctt ggtctctttg
     8821 taagactttt cttccagtac cggttccact atatttttag ctaccatcgt actgcaaacg
     8881 ggcaggtaac taaaatcatt ggggtactta atttcctttc ggagttctgt cccttcgctg
     8941 tcgtaaattt ttgtttgagt aggtaatata ttattcgcac tgccataggt aaagagtacc
     9001 tctgaagtga ttactttatt attcaccagg tcatactctt ctgtaaccgt cctgtctaaa
     9061 tgatcccatc gggtaaatat gctgtaactc gtaacatcca ggtgctgaaa atcgtaatca
     9121 tcccatggtg gtgttccggc taacataggg ctgaatcgct tgcgaacgat atagttattt
     9181 aattcagcat cattgattcg ggaatctgta ctgtaataat tcgttatctt ttttacaaac
     9241 tccatgttat ggttaaaaaa ccgggtctgg ctctctaggc cattcatata gttatagggg
     9301 tccaccgggg gattgggtac cgtctctccg atgatctggt tgccaataaa ttgtccctgt
     9361 ttgataaacg tatgctcgac cccaccgttg gagaataggg aatcatcact ttctattacc
     9421 catttataaa ccacaccccc ggcttcatta tcatacaagg tggtagcact gttcgaactc
     9481 agcaggaatc cattacataa tatttcaagg ttactcgacc cgcaatacag tccgttggaa
     9541 taagcagtaa catattgagg ataatacagt ttctgagccg aaggattcaa agtatcggtt
     9601 aacgaacggt aatgataaaa ctttgaatgg gactttcttg aaacattatc ataggaaaca
     9661 atttttctta ctcgtactcc gcataactct tcattgatct gtccccattc tgtcccgcat
     9721 aactggtcat agacaatatc tgcccttgcc ttattattag ggccattccg tacttccagt
     9781 tcaaagcgat agttaatccc ggggcttaag ggaacatgaa aatcccccca tacttcatga
     9841 ttgggaaaac ctgaatatcc aaatgctgtt ctcttttcta tcacaagacc tgtactatta
     9901 tcatataaag tagcaatggc aacctttggc gcagggtcac ccccaggtac tggtaaaggc
     9961 ccgatccact ctgtactcaa atgaaactcc ccggtttgtg cctcacatac ggtgaaatag
    10021 gtattgtaaa actgggaggg atatcctcct gtttctgtca gccttgcact tattattttc
    10081 ttcttttcaa tattgcccca ccatgttttc atattgggtt cataatgaaa ttcatcatgc
    10141 ccgccggtgg gatatactat tctctggagc atcccttttt gtgcataaac accgttgggt
    10201 tcacggttag ctgtaccata agaagcaaat aaaggaaagc cattatatac ataggtattt
    10261 acaacaggca gtaatgttga attacttgcg ccgttggcga aaccaaggtg atcaatatta
    10321 ttggtgattg cggcccccgc caatgcattt tcttcatcta cataatgtaa ttcgaaaacc
    10381 tgctcctcat tgctgcttgt agtaggatct ttaagtatta cttttttcaa aaagaatcct
    10441 tcattaaagg ggggattggg tatatgcctg tccaggtatt gaaacacggc ctttcgaagg
    10501 gtcgttccgt aaccgttgga taccagaatc tggttcaccc tgttatcacc acctgcatca
    10561 ggccggtcct cgtacaggat agcaaccgca gtaccggtat ttgtattaat agcggtaagg
    10621 tagcggctgg aataacggat tgtatttgtt ctttctgaat actgtgcttc taccggacag
    10681 gccagtgttt cattacatgc cggccccagg atgccttttc gtactgacta tattacgccg
    10741 gaattgacat aatgcgtaag actgccataa ctaaaatcga tataatctcc attgggaagt
    10801 gttacattgg tcagcatcca tgccgttgta atagtttgct tttgcagcag atcataaacc
    10861 aggttatgct ccagggtact ttcctgcact ccgcccccaa acgtatactt cacaccacct
    10921 ccatccgtga tgataatatc cgtaacggct ccctggcttt tcaaaacaat aatttttaca
    10981 ttattataag gcaactgcaa tacagaacca tcccgtttga taatgaactt acccgttagt
    11041 ccgttcacag tatatcggta ttcgtctggc tgagaatcat gattactgtt ctgatcttct
    11101 aaatcctcaa ggaagaaaaa agaattcgaa ttctttgcca gcacctgcga ttctgttaga
    11161 tactgagccg gtggagaaca ataatcatcc ggctttcctt taacaatacg ggttactgca
    11221 ccacctgcat tcaggctcca tgataatcct accctggatg ggatttcttc aggcttaaac
    11281 cctgttgaag agtagttaac acttataggc agagaaaaat ttctcagctt taattcataa
    11341 ataggaatac tagaattagc tcccccggta tgaggcgtag atcggagttc agctcccttt
    11401 gtcagtgttg ccaaatctgg tgatggtggc agcacctcgg gcaacttgaa cgtattgtta
    11461 cttccttgtg cttctatctc aatgtaaaag aataataaaa gaataatgga tgtaaaaaat
    11521 ctcatattat ttcatttaaa aattataccc tatcctgaaa atcaccggct gtgttcttgg
    11581 cacctgctgg taactcaaaa aatcccacaa cagttttagc gatgtcttct taaagaactt
    11641 actctttacc ggcaccgttt tactgatacc gatcagtccg ctctgctgcc acgatgaata
    11701 gttttgcagc acattaaaat tcctgaactc acttctgtaa ttcatctcaa aaccgccgct
    11761 gacccataga gatcctttca gcttccagtc tataaaactt cgggctgata atccctggtg
    11821 actgatacgg atattgcgca aattctgccc ccatcccatt ttataagaag cgccaagccc
    11881 cactatgctt ttatcattca gtttataccc ggcacttacg cctatatcac tggtcaacgg
    11941 taaaaaacca gtggatttct gtgactgtat atttgtaccc agctcaatcc ttttcagaaa
    12001 tgactttgtt ttctggttat tgggcttgaa tccttccggc atttcagcat tactgcttcc
    12061 tccgcctgct tttaatattt tatcctttaa ctcattcaac tgcgattgtg ccgcctgcag
    12121 gttctgctga aactgtgcct gggcattggg gccaccggca gctatctgtg tttgtataag
    12181 actattcacc tgtgcccttg tctgtaatcc ggcaagactg gctacataag caggatcatt
    12241 gggatccccc ggcattctga acaacgaagc cagcatacta ttctttctca taaaatccct
    12301 gaacagtttt gtctttgcca gcagttccag cacctttttc tccgtcttct tcgggtactt
    12361 taatatctct ttatattcat tcagctgctg ggcataataa tac
//
LOCUS       DAORTI010000106        12280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5205_length_12280_cov_5.409571, whole genome shotgun sequence.
ACCESSION   DAORTI010000106 DAORTI010000000
VERSION     DAORTI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_12280_cov_5.409571"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..744)
                     /locus_tag="PLO70_12460"
     CDS             complement(403..744)
                     /locus_tag="PLO70_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQZ75323.1"
                     /translation="MHKAYLTNQTFDKSDFTRNPIPNADFEACCFTHSNFSDTDLSGY
                     NFTDYIFEDCNISTARIVQTTFNEVVFRNCKMLGLHFETANEFLFTVSFENCNLNLCS
                     FYQRKMKKNHI"
     gene            903..2696
                     /gene="lepA"
                     /locus_tag="PLO70_12465"
     CDS             903..2696
                     /gene="lepA"
                     /locus_tag="PLO70_12465"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQZ75324.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLQSTGTISDRDMMDQVLDDM
                     DLEREKGITIKSHAIQINYKHTDGQEYILNLIDTPGHVDFSYEVSRALAACEGCLLLV
                     DATQGIQAQTISNLYLAIENNLEIIPVVNKIDMAGAMIEEVEDQIIELIGCKREDILH
                     ASGRTGLGVEAILDAIVNRIPAPTGKPDAPLQALIFDSMFNPFRGIIVYFRVRNGSIK
                     KGDKVKFVSTDQEYDADEVGILKLKMTEKKELKCGDVGYLITGIKNAKEVKVGDTITL
                     AANPTQEIIQGFEEVKPMVFAGIFPVNTDDFEELRDCMDKLQLNDASLTYELETSQAL
                     GFGFRCGFLGLLHMEIIQERLEREFNQTVITTVPNVSFIAYTTKGEKVIVNNPTEFPD
                     PVKTDHIDEPFIRAQIISKPEYIGNIMTLCLGKRGILMNQSYLTTTRVELIFEMPLTE
                     IVFDFYDKLKSQTRGYASFDYHPIGYREADIVRMDILLNNDKVDALSALIHRSRAQEF
                     GRKLCEKLKELLPRQQFQIAIQAAIGAKVVARENISAMRKDVTAKCYGGDISRKRKLL
                     EKQKEGKKRMRQIGNVEVPQEAFLAVLKLDD"
     gene            complement(2835..3920)
                     /locus_tag="PLO70_12470"
     CDS             complement(2835..3920)
                     /locus_tag="PLO70_12470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQZ75325.1"
                     /translation="MATSNHIVILCSRLDLPGGIERAVVNTANLFCEKGHSVSLVILD
                     EARNSFYPVHPSVEIIHHSLSFGITPEGNVISRKIKLLTDVLKLRRLLKQLHPDIVIA
                     SEYPFAAAIILSGSHKIAKAISWEHHHFYELKRNRFWHKIFKLTYPWLEAVVCLNNDE
                     KRLFETINPKTTVIPNFIKAAARSELANKTILTVGRLTAVKGADLLLQTAKLVLQQHP
                     GWKWKLIGDGDLKEEVLHFIEKENMQKQLIIQPPLHHNIIMEYQNASLYVMTSRNECF
                     PMTLLEAQSAGLPCISFDCDSGPRHIIKNEENGILVEKENTLQLAAAVSSLIMDEAKR
                     KKMGELALNNIQEFSPGKIYQLWKELF"
     gene            complement(3924..4880)
                     /locus_tag="PLO70_12475"
     CDS             complement(3924..4880)
                     /locus_tag="PLO70_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75326.1"
                     /translation="MNTFCTIINRQYLPFASVLFQSLAKTEPGAQLQVLVTDVEGGSM
                     LSSPFPGLTYYSQADILNSSMANNIYQKYAASNTDILRWALKPVFICHLLQKIFEKVI
                     FVDPDMYFVSHHSFLFDALNEHAVLLSPHWSSMDPLKNEDGLYSVLRNGLYSGGFVAA
                     SKKGMMAMEWWAGVCHYKMERRQELGLYDDQKYLDMIPLIEKTTCIIRHKGCNLASIN
                     IDSCKREMINNKLLINQEFEPVFIHFTKDTIINIANRNDHLLKPFLDNYIVELAAENF
                     DLPSKLDTMDTATFNSAMYAVKHKARLRTRLKRFFFRLAEKI"
     gene            complement(4903..5733)
                     /locus_tag="PLO70_12480"
     CDS             complement(4903..5733)
                     /locus_tag="PLO70_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQZ75327.1"
                     /translation="MSNNLILRLARKIKIPFIRKGLLRFLFKKNGLHLQQLYSFNHDK
                     FFIYQVNNHFIPNEALTTFISYETQSDKCRNESLFAYQPQEGDTIIDLGAGLGEETLY
                     YSHLVGHTGKVISVEANPAVFEVLQKTITLNGLSNVSSFNMAVFSDNGTTSLSVNDPS
                     YEAAFITKDTKKAINIPASRMDSFLEKQKIEKISLLKANIEGAERYIIDTLTAQQVSK
                     IRHIAIACHDFRYLREGNDFYKTKKLVIDFFTKNGFEISTRQTGIDYLDDWVFGTNKL
                     "
     gene            complement(5726..6817)
                     /locus_tag="PLO70_12485"
     CDS             complement(5726..6817)
                     /locus_tag="PLO70_12485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQZ75328.1"
                     /translation="MKVLGLISSLTDPSSRARILQYKDYLEKENISLHPKYFSPYNYA
                     DPAPWMYRLNKITKINPWRFAWLQKTASRLPLLLQQHRYDLIWQNRLVLPQQSFYEKK
                     FTKSVVFDFDDAIWLHDGEKNVAEAIAKADMVFAGNDYLADFALKHNQHVTIIPSVID
                     TSKLFPVPEKLKPFTIGWIGSISNYRFLELVKPAITDFLTANKDSRFIIVSSEKKGPF
                     EFDDKRILFRKWSAEKENELINEFSIGLMPLTDDDFTRGKCSYKMLQYMACGKPVVVS
                     PVGMNNKILATDTVGLPAVKNEDWLNAFEILKNDFSFYKTCAEKGRGLVEREYSINKY
                     CPVISNHFKKITGGETSRNKLRRHKKGNE"
     gene            complement(6826..7665)
                     /locus_tag="PLO70_12490"
     CDS             complement(6826..7665)
                     /locus_tag="PLO70_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ75329.1"
                     /translation="MTKKTVPTGERLTEFALHVITLEHLHRYAIAIELSKNKSVLDIA
                     CGEGYGSHLLALQSSSVTGVDIDTVTIEAAKIKYQHPHLNFLSGNVENISVATASFDV
                     AVSFETLEHITDHDKMVAELKRVLKPDGVLLISTPDKLNYSDKPGYHNSFHKKELYKT
                     EFEILLLQYFKNVQLLSQTSGLVSLITPQTTTGRSISYTGDFSDIQSEKDLGAPFLIA
                     IASDNLLPEIPLSIFKSETLLPLLLQQQRDAIKETASYKVGHILLSPFKIIRNLLRKK
                     EQQ"
     gene            complement(7699..8952)
                     /locus_tag="PLO70_12495"
     CDS             complement(7699..8952)
                     /locus_tag="PLO70_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ75330.1"
                     /translation="MKPAIEIRNLGKEYKLAASEPYMSLRDTISGLVKKIFQPGRKKE
                     QRFWALQNIDLTIQPGERIGIIGRNGAGKSTLLKIISRITPPTTGEAIIRGRVGSLLE
                     VGTGFHPELTGRENIYLNGSILGLKKAEINKQLDAIIDFSGVEKFIDSPLKHYSSGMQ
                     LRLAFSVAAHLEPEILLIDEVLAVGDMEFQKKCLGKMEEVSKSDGRTILFVSHNLNFV
                     SGLCNKAVLLNEGKLINTGSTTEVLGEYINSIVKRHIDKNWDTNPPGNDIVRLRQVQL
                     VSQDGENKENFSVSEPVGIQMTYEVLQGGHVLWLGHNIHNQEGINVFDTHNVNSPYYT
                     QTSEKGIYKATAWIPANFLNTGNFLISTAIFNHLQKTIHLHERDVLFFTVNEVFEGKT
                     ARGMSPGDFPGVIRPVLKWDTVKLN"
     gene            complement(8952..9776)
                     /locus_tag="PLO70_12500"
     CDS             complement(8952..9776)
                     /locus_tag="PLO70_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ75331.1"
                     /translation="MEKFITHIQPPKGFSLGLKELWQYRELFYFFTWRDIKVKYKQTY
                     LGILWALLQPLGMMALFTLLFSKTWKIDTGTTAYPIFVLSGLILWNLFSSSVSHAGES
                     MIQNANIIRKIYFPRLIIPGSSVLVALFDFLMGLFVFFIFCYIYNQPLHWYSVFYFAA
                     GILIILIAGFGAGTFLAALNVKYRDFRYTIPFLLQVLFFSSQVIYPLYSVQQTWLKYL
                     LAINPVNAAIELFRATLSNHPIDSTTISIGLTSTVIIFFVGLYYFRKTEAYFADLA"
     gene            complement(9830..10846)
                     /locus_tag="PLO70_12505"
     CDS             complement(9830..10846)
                     /locus_tag="PLO70_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75332.1"
                     /translation="MTKTILVKKLLIQIAFLILWLLSFSQVALSQKITQADLKKLRAK
                     EDTLSEYSEYLNTDSLPEDRMIADSAFTKVLVRALQVKNSFYYPFDSVLGVSKLYATD
                     TSFRIITWNINFDDYYSRQKGAIQFRTTDGSLKLLPLRDVSEFTDKPQDSVRSRQNWI
                     GSIYYNIIKTQHKGKNYYTLFGFDNNSAQSSMKWIEVLSFNEKNEPVFGGPFFSFEKD
                     SVPKPPKYRLGLEFKKGARVLVNYIEDLGMILVDHLISESDQPELAWTYVPDGDQEGF
                     KWENGKWMHIDKVFTLKLEDGQAPVGDPLMDPKGNKNEQKLQEKTDKNKAKEGKRIPL
                     NYDN"
     gene            complement(10916..12160)
                     /locus_tag="PLO70_12510"
     CDS             complement(10916..12160)
                     /locus_tag="PLO70_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQZ75333.1"
                     /translation="MIKEAIILAGGLGTRLRESVPDLPKCMAPVAGRPFLFHVINYLR
                     SQGVEKFIFSLGYKHELIEDYLSTQFSTLSYQCVIEEEPLGTGGAILLACTKATEKNI
                     VVTNGDTLFKIDLHKATMFHNHNNAECTLLLKPMNRFDRYGVVELDEEQLVNSFKEKQ
                     FYETGNINGGVYLLNKDKFLDEEFPAKFSFEKHYLEKFYPVRRIYGLIQDAYFIDIGI
                     PEDYNRAQSELSRPPLDLSTIDKSWTLFIDRDGVINHEKKEDYILNWEEFRFYEGVEK
                     AFQKLAGKFGKIIIVSNQRGVGKQLMTEHDLRTIHQNMQNAIEAAGGRIDKIYYCTST
                     DNKHPDRKPNPGMAFHAKNYFDSVDLSRSVIIGNKPSDMLFGKNAGIYSVFLATTNPD
                     VPFPHPDIDLRFDSLPDFAKAL"
BASE COUNT         3555 a         2335 c         2508 g         3882 t
ORIGIN      
        1 tacagcaatc gccgggttta ttgcggtaca atttgttttt attacccggt taatcggtat
       61 aattcaaagc caatgccagt gtgccagaat ctggtaaagg aaatgttaag gatagattag
      121 tgataaaagt attctggaga ctataaaggg gctgtatata aatacaatta ttgttctact
      181 atgatatcat atttgtcaag caggcgaata acagagggta aagaaaattt cccttttttt
      241 atcttattaa cttccggatc aatggaatag ttgaaggatg ttcgcaggtc agctttctct
      301 aaattcgaat gatcaaattt ggcatcttgc aggtcacagt tagagaatat ggctgctgtt
      361 aagttggttt cagtaaaatc agcttcctgt aaactgcagt ggttaaatat gatttttttt
      421 cattttacgc tgataaaaag agcacaggtt gagattgcag ttttcaaagc taacggtaaa
      481 aaggaattca ttagctgttt cgaaatgcag cccaagcatt ttacaattcc tgaatactac
      541 ttcattgaag gtggtttgta cgatcctggc ggtactgata ttacaatctt caaaaatgta
      601 gtcagtaaaa ttataaccgg ataaatcagt atcagaaaaa ttgctgtggg tgaagcagca
      661 ggcttcgaaa tctgcattag gtatggggtt gcgggtaaag tctgacttgt caaaggtttg
      721 gttggtgagg taggccttgt gcatatgcat agtatgaaat ggccgcaaga taccctctag
      781 aatcaaaagc ctctaactgc agagatattg ctgttgaagg gatgagagaa cgtctaaaca
      841 atcccttcag aaccgcctct tttccgtacc ttcgccacct cgaaaaatga ggtaaaacgt
      901 acatgaagaa tatccgcaat ttttgcatta tcgcccatat tgatcacggt aaatccaccc
      961 tggctgaccg gttattacaa agcaccggta ccatcagcga cagggatatg atggaccagg
     1021 tgctggatga tatggacctg gaaagggaaa agggcattac tattaaaagt catgctatcc
     1081 agatcaacta taagcatacc gacgggcagg agtatatcct gaacctgatc gatactcccg
     1141 gccacgttga cttcagttat gaagtgagcc gggcactggc tgcctgcgaa ggctgcctgc
     1201 tgttagtgga tgctacacaa ggcattcagg cacagactat cagtaactta tatcttgcaa
     1261 ttgaaaataa tcttgagatt attcctgtgg tcaataagat cgatatggca ggagccatga
     1321 ttgaagaagt ggaagaccag atcattgaac tgattgggtg taaaagggaa gatattctgc
     1381 atgccagcgg ccgtaccgga ctgggtgtgg aggctatcct ggatgctatt gtaaacagga
     1441 ttcctgcgcc caccggtaaa ccggatgcac cattgcaggc gttgattttt gacagtatgt
     1501 tcaatccctt tcgtggtatt attgtttatt tccgggtaag aaatggttcg ataaagaaag
     1561 gtgataaagt aaaatttgtt tccaccgacc aggaatacga tgcagatgaa gtgggtatcc
     1621 tgaaactgaa gatgacggaa aaaaaggaac taaaatgcgg cgatgtgggt tacctgatta
     1681 ccggcattaa gaatgcgaaa gaagtaaaag tgggggatac gataaccctg gcggccaatc
     1741 caacacagga gatcatacag ggctttgaag aagtgaagcc aatggtattt gccggtattt
     1801 tcccggtgaa tacggatgac tttgaagagt tgcgggattg tatggataag ttgcaattga
     1861 atgatgcatc gctgacctat gaactggaga catcgcaagc ccttggtttt ggtttccgtt
     1921 gcggctttct tgggttgctg cacatggaga tcatccagga aagattagag agagaattta
     1981 accagacggt gatcacaacc gttcccaacg taagttttat tgcctataca accaagggag
     2041 agaaagtgat cgttaataat ccaaccgagt ttcccgaccc ggtaaaaacg gatcatattg
     2101 atgaaccctt tatcagggcg cagattatta gtaagccgga gtatatcggt aacatcatga
     2161 cactttgttt gggtaaacgg ggcatactga tgaaccaaag ctacctgact acaacaaggg
     2221 tggaactgat ctttgaaatg ccattgacgg aaatagtttt tgacttttac gataagctga
     2281 agtcacaaac gaggggttat gcatcgtttg attatcatcc gataggttac cgggaagcgg
     2341 atattgtacg aatggatatt ttattgaaca atgataaagt agatgcattg agtgcattga
     2401 ttcaccgcag ccgggcacag gagtttggac gaaaactctg cgagaaactg aaagaattat
     2461 tgccaagaca gcaattccag attgccatac aggcggccat tggtgctaaa gtggtagcaa
     2521 gagagaatat ctctgcgatg cggaaggatg taaccgccaa atgttatggt ggcgatatca
     2581 gccggaagcg taaattgttg gaaaagcaga aagaaggtaa gaagcggatg cgccagatcg
     2641 gaaatgtaga agtgccgcag gaggcttttt tagcggtgtt gaagctggat gattgatttg
     2701 aggagttaaa ttgcgggaat atatttatag gcatcaggaa tggtgccttt tttattttcg
     2761 ggctgtttcc gatgattttc tcagcgttca tctgcggtta gcatctgtgt aatctgcggg
     2821 aaaacttcat ttgatcagaa caactctttc cacaactggt aaatttttcc aggtgagaat
     2881 tcctgaatat tattaagcgc taattctccc attttctttc tctttgcttc atccatgatc
     2941 agcgaagaaa cagctgcagc cagttgtaag gtattttctt tttcaacgag tattccattt
     3001 tcctcatttt tgataatatg ccttgggccg gagtcgcaat caaaggaaat gcagggcagg
     3061 ccggcggatt gtgcttccag caatgtcatt gggaaacatt cattccggga agtcattaca
     3121 tacaatgaag cattctggta ttccattatt atattatgat ggaggggcgg ctgaatgatt
     3181 agctgttttt gcatgttttc tttttcgata aaatgcagca cttcctcttt caggtctcca
     3241 tcaccgatca gtttccattt ccagccggga tgttgctgta aaaccagctt tgctgtttgt
     3301 aatagcaggt cagctccttt tacagcagta agccttccga ctgtgaggat tgttttattg
     3361 gccagttctg atctggctgc tgcttttatg aaattgggaa tgacagttgt tttcgggttg
     3421 atagtctcaa agagcctttt ttcatcatta ttgagacaaa ctactgcctc cagccaggga
     3481 taagtgagtt tgaatatttt atgccagaat cgatttcttt ttaattcata gaaatgatga
     3541 tgttcccatg aaatagcttt tgcaatttta tgtgaaccgg ataaaataat agcggcagca
     3601 aaaggatatt cggatgcgat gacaatatcg ggatgcaatt gtttcaaaag ccttctcagc
     3661 ttcagtacat ctgtcaacaa ttttattttc cgggaaatca cattgccttc gggagtgatg
     3721 ccaaatgaca acgaatggtg tattatttca acggaaggat gaacaggata gaagctgttc
     3781 cttgcttcat ctaatattac caatgataca gaatggcctt tttcgcaaaa cagattggca
     3841 gtgttgacaa ctgctctttc gataccaccc ggcagatcaa gccgggagca gaggattacg
     3901 atatgattag aagtagccaa gaattatatt ttttctgcta gcctgaaaaa gaatctttta
     3961 agtctggtgc gaagccttgc tttgtgttta acagcataca tggcagagtt aaatgtggct
     4021 gtatccatcg tatccagttt gctcgggagg tcgaaatttt ctgccgccag ttcaacaatg
     4081 tagttatcta aaaaaggttt taagagatgg tcattcctgt ttgctatgtt tataattgtg
     4141 tctttggtaa aatgaataaa cactggctca aattcctggt taatcaacaa tttattatta
     4201 atcatttctc ttttgcagga atcaatattg atactggcaa gattgcagcc tttatgccgg
     4261 atgatgcagg tagttttctc tatcaaaggt atcatgtcta gatacttctg atcatcatac
     4321 agtccaagtt cctggcgccg ttccatttta taatgacaga caccggccca ccattccatt
     4381 gccatcattc ctttcttgct ggctgccaca aaacctccac tgtacaaacc gtttctcaga
     4441 acagaataga ggccatcttc attttttaat gggtccatac tactccagtg aggtgaaagt
     4501 aatacagcat gttcatttaa tgcatcaaaa agaaaactgt gatggcttac gaaatacata
     4561 tcgggatcaa caaatattac cttttcaaat attttttgta aaaggtggca gataaacact
     4621 ggctttaacg cccagcgaag aatgtctgta ttagaggcag catatttctg gtaaatatta
     4681 ttagccatgg aactatttaa aatatcagcc tgcgaataat aggtaaggcc gggaaaaggg
     4741 gaggaaagca tgctgccgcc ttcaacgtca gttacgagta cttgtaattg tgccccaggt
     4801 tctgtttttg ccagcgattg aaaaagaacc gatgcaaacg gcagatattg tctgtttatt
     4861 atcgtacaaa aagtattcat cttactaata gtagcaaagt atttaaagct tattggtacc
     4921 gaaaacccag tcatcaagat agtctatacc ggtttggcgg gtacttattt caaaaccatt
     4981 ttttgtaaaa aaatcaataa ctagtttttt agttttgtaa aaatcattgc cttctctaag
     5041 gtatcgaaaa tcatgacagg caattgcgat atgcctgatt tttgatacct gttgggccgt
     5101 aagtgtatct atgatatatc tctctgcacc ttcgatattt gcttttagca ggctgatctt
     5161 ttctattttc tgtttttcaa gaaaactatc catgcgactg gctggtatat taatagcttt
     5221 cttagtgtct tttgtaataa atgcagcctc atatgatggg tcattaacag aaagacttgt
     5281 tgttccgttg tcagaaaata ctgccatatt aaaggaagaa acgttgctca gcccgttaag
     5341 ggtaattgtt ttttgcaata cttcaaatac tgccggattt gcttccacgg atattacttt
     5401 tccagtatga ccaacaagat gggaataata cagtgtttct tcacctaagc cagctcctaa
     5461 atcaattatg gtatcgcctt cttgcggttg ataagcgaag aggctttcgt ttctgcattt
     5521 atcagattga gtttcgtagc tgataaatgt tgttaacgcc tcgttgggta taaagtggtt
     5581 attaacctgg taaatgaaaa acttgtcatg gttaaaagaa tagagctgct gcaggtgaag
     5641 tccgtttttt ttaaataaaa accgaagtaa cccttttctt ataaaaggta tttttatctt
     5701 tctggctaac cttagaatta aattattact catttccttt tttatgcctc cgtaatttat
     5761 ttcggcttgt ttctccgccg gttatttttt tgaaatgatt agaaattacc gggcaatatt
     5821 tgtttattga atattctctt tcaactaaac ctctgccttt ttcggcacag gtcttataaa
     5881 aggagaaatc attttttagt atttcaaatg cattgagcca gtcttcattt tttactgccg
     5941 gcaatcccac tgtatccgta gccaggattt tgttattcat tcctacaggg cttactacta
     6001 ctggtttgcc acaggccatg tattgcagca ttttatagct gcattttcct ctggtgaaat
     6061 catcgtcagt aagcggcata agaccaatgg aaaattcatt aattagctca ttttcttttt
     6121 cagcagacca ttttctaaat aatattcttt tgtcatcaaa ttcaaatggc cccttttttt
     6181 cagatgaaac tattataaac cgcgaatctt tgtttgcggt caggaagtcg gtaatagctg
     6241 gcttaacgag ttccagaaac ctgtagtttg aaatactacc tatccagccg atagtaaatg
     6301 gcttaagttt ttctggaaca ggaaaaagtt tgctggtatc aattacagaa ggaataatgg
     6361 ttacatgctg attatgttta agtgcaaaat cagccagata atcattaccg gcaaatacca
     6421 tgtcagcttt agcaatagcc tcagctacat ttttttcacc atcatgcagc caaatggcat
     6481 catcaaaatc aaatacaacc gatttggtaa attttttttc gtaaaatgat tgctgtggta
     6541 ataccagcct gttttgccag attaaatcat acctgtgttg ttgtaaaaga agaggtaacc
     6601 tgctggctgt tttttgtaac catgcgaatc gccaggggtt tatttttgtt attttattca
     6661 gccggtacat ccatggagcc gggtctgcat agttatacgg agaaaaatat tttggatgca
     6721 gagaaatatt ttccttctcc agataatctt tatactgaag aattcttgcc cttgatgagg
     6781 gatctgttag tgaagatatg agacccaata ctttcattca cttctttatt gctgctcttt
     6841 tttacgtaaa agattcctga tgattttaaa cggggatagt aatatatggc cgactttata
     6901 gcttgccgtt tcttttattg catctctttg ttgttgtaaa agcaacggta ataatgtttc
     6961 tgacttaaaa atacttagtg gtatttctgg cagcaggtta tctgatgcga ttgcaataag
     7021 aaaaggtgcc cctaaatctt tttctgattg aatgtcacta aaatcaccgg tatagctgat
     7081 tgatctgccg gttgttgttt gcggcgttat cagagaaacc aatcctgagg tctgtgaaag
     7141 taattgcaca ttcttaaaat attgaagcag gagtatttca aattcagttt tgtacaattc
     7201 ttttttatga aaagaattat gataccctgg cttatcacta taattcaatt tgtccggagt
     7261 ggagataagt aagacgccgt ctggttttag aactcttttt aactctgcta ccattttatc
     7321 atggtctgtt atatgttcca gcgtttcaaa acttactgcc acatcaaaac ttgcagtggc
     7381 tacagaaata ttttccacat tacctgataa aaaattaaga tgaggatgtt ggtattttat
     7441 tttagcagcc tcaatagtta ctgtatcaat atctactcct gttactgatg aagactgtaa
     7501 ggccagcagg tgggagccgt atccttcacc gcaagcaata tcaagaactg atttgttttt
     7561 tgacaactca attgcaatag cataacgatg taaatgctcc agcgttatta catgcagggc
     7621 aaattctgta agccgttcac cagtaggtac agttttcttt gtcatatatt gttatatcat
     7681 caaaacggat aacagtcttt aatttagttt gacagtatcc cattttagaa caggcctgat
     7741 tacaccgggg aaatctccgg gactcatacc ccgggccgtc tttccttcaa atacttcgtt
     7801 tactgtaaag aaaagtacat ccctttcatg caggtggatt gttttttgca agtgattaaa
     7861 aattgccgta ctaatcagaa aattcccggt atttaaaaaa ttagctggta tccaggcggt
     7921 ggccttataa attccttttt cagaagtctg ggtataatat gggctattta cattgtgagt
     7981 gtcaaataca tttatccctt cctggttatg aatattatga cccagccaca gtacatgtcc
     8041 accctgcaac acttcatatg tcatttgtat gccgactggc tcagataccg aaaaattctc
     8101 tttattctca ccatcttgcg aaactaattg cacctgcctg agccggacaa tatcattacc
     8161 cggtggattt gtatcccagt ttttatcaat atggcgtttt actattgaat taatatattc
     8221 tcccaatacc tctgtagtag aaccagtatt aattaatttg ccctcattca gtaagacggc
     8281 tttgttgcaa aggcctgata caaaattgag attatgactt acaaataata tagttcgtcc
     8341 gtcacttttg cttacttctt ccattttacc caggcatttt ttctggaatt ccatatcgcc
     8401 aacagccagt acctcgtcaa tcaaaagtat ttccggttct aaatgagcag caactgaaaa
     8461 ggctagtcgc aattgcatac cactgctgta atgtttaagt ggtgaatcaa taaatttttc
     8521 caccccacta aaatcaataa tggcatccaa ttgtttattg atctctgctt tttttaagcc
     8581 aagtatggag ccattcaggt aaatattctc tcttccggtt aattcaggat ggaaacctgt
     8641 tcctacttcc agtaaacttc ccactcttcc cctgatgatt gcttctccgg ttgttggtgg
     8701 ggtgatgcgg ctgatgattt ttaataaagt gctttttcct gcaccatttc ggccaatgat
     8761 gccaattctt tctccaggct ggatagtaag atcaatattt tgtaatgccc agaatcgttg
     8821 ttcctttttt ctacccggct gaaatatttt ttttaccaag ccggaaatag tatcccttaa
     8881 agacatatag ggttctgatg cagccagctt atattctttg cccaggtttc gtatttcaat
     8941 agcaggtttc attatgctaa atctgcaaag tatgcttctg tttttctgaa ataatataaa
     9001 ccaacgaaaa aaatgataac cgtactggta agcccaattg atattgttgt tgaatcaatg
     9061 ggatgatttg ataaggttgc cctgaataat tcaatagctg cgttaacggg gttaatagct
     9121 aataaatatt tcagccaggt ttgttgcaca gaataaagcg ggtaaataac ctgtgatgaa
     9181 aagaacaata cctgcagtaa aaagggaatg gtgtacctga aatcccggta cttcacattt
     9241 agtgcagcga gaaatgtccc ggcaccaaaa ccagcaatga gtatgatcaa tataccagct
     9301 gcaaaataga atactgaata ccagtgcagg ggctggttgt agatgtaaca aaaaataaaa
     9361 aaaacaaata atcccattaa aaaatcaaat agggcaacca gtactgatga gccaggtatg
     9421 atcaatcttg gaaaatagat tttcctgatg atattggcat tctgtatcat gctttctccg
     9481 gcatgtgaga cagaggagct aaagagattc cataagatca gcccggataa aacaaaaata
     9541 ggataggctg tagtgccggt atcgattttc caggtttttg agaaaagtaa agtgaaaagt
     9601 gccatcatgc ccagcggctg caagagtgcc caaagaatac cgagataggt ttgtttgtat
     9661 tttactttga tatcccgcca ggtgaagaag taaaacaact cccggtactg ccaaagttct
     9721 tttaaaccaa gagagaaacc tttaggtggc tgaatatgag tgatgaactt ctccatgagt
     9781 tgtaaagata cccattggag actgaaacgt aaagacttaa gcgaacagtt tagttatcgt
     9841 aattaagtgg aattctcttg ccttcttttg ctttgttttt atccgttttt tcctgcagtt
     9901 tttgttcgtt cttatttcct ttcggatcca tcaacggatc gccaaccggc gcctgtccgt
     9961 cttccagttt taacgtaaag actttgtcaa tatgcatcca tttgccattc tcccacttaa
    10021 atccttcctg gtcaccatcg ggtacatagg tccaggcaag ttctggctga tccgattcag
    10081 aaatcaaatg atccaccagt atcattccaa gatcttcaat ataatttacc agcaccctgg
    10141 cacctttttt aaattcaagg cctaacctgt acttgggcgg tttgggaacc gaatcttttt
    10201 caaaagagaa aaaaggaccc ccaaagaccg gttcattttt ttcattaaaa cttaatactt
    10261 caatccactt catactgctt tgtgcactgt tattatcaaa tccgaaaagc gtataatagt
    10321 ttttcccttt gtgttgggtt ttgatgatat tataataaat agatccaatc cagttttgcc
    10381 tgctgcgtac agaatcctgg ggtttgtccg taaactcaga tacgtctctc aggggtaaaa
    10441 gtttcagtga accatcggta gtacggaatt gtattgctcc cttttgccgg gagtaatagt
    10501 catcgaaatt gatattccag gtgatgatcc gaaaactggt atcagtagca tataatttag
    10561 aaacacccaa tactgaatca aaagggtagt aaaatgaatt tttaacctgc agggctctta
    10621 ccaatacttt agtaaatgca ctgtcagcaa tcatcctgtc ttccggaagg ctgtcagtgt
    10681 tcagatattc tgaatattca ctcagtgtat cttcttttgc cctgagcttt ttcagatcgg
    10741 cctgggtaat tttttgtgac agggcaacct gcgaaaagga tagcagccaa agaataagga
    10801 aagctatttg aatcagtaat ttctttacca ggatggtttt tgtcatagtc atgctaagtt
    10861 acagcttaat acagatgccg ataacgctaa aagggttgta aaattttgtg aagactcata
    10921 aagctttggc aaaatcaggg agggaatcaa accgcaggtc aatatcaggg tgggggaaag
    10981 gaacatcggg attggtagtg gccaggaaaa cagaataaat gccggcattt ttgccaaata
    11041 gcatatcgct gggtttatta ccaattataa cagaccttga aaggtcaact gaatcaaaat
    11101 aatttttagc atggaatgcc atacccgggt taggcttcct gtcggggtgt ttattatcgg
    11161 tactggtaca atagtatatt ttatctatcc ttccaccggc ggcttctatc gcattttgca
    11221 tattctggtg aatcgttctc aggtcatgtt ctgtcatcaa ttgtttgccc actcctcgtt
    11281 ggttgctcac gatgatgatt tttccaaatt tacctgccag tttttgaaat gctttttcaa
    11341 caccctcata aaaccggaat tcctcccagt tgagaatata atcttctttc ttttcatgat
    11401 tgatcacacc atcccggtca ataaatagcg tccagctttt atcaatagtt gataagtcaa
    11461 gtggggggcg actgagctct gattgtgcac ggttgtaatc ttccggtatg ccgatatcaa
    11521 taaaataagc atcctggatt aatccataaa tacgccgtac cgggtaaaat ttttccaggt
    11581 aatgtttttc aaacgaaaac ttagccggaa attcttcatc caggaatttg tccttattca
    11641 gcaggtatac acccccgttt atattacccg tctcataaaa ctgtttttct ttaaaactat
    11701 ttaccaattg ttcctcatcg agctccacaa caccatagcg gtcgaacctg ttcatcggtt
    11761 tcagtaacag ggtacattct gcgttattat gattatggaa catcgttgct ttatgcaggt
    11821 caattttaaa cagggtgtcc ccatttgtaa caacaatatt cttttcagtt gcttttgtac
    11881 aggccaatag tattgctccg cctgttccca ggggttcttc ctcaataaca cattgatagc
    11941 tgagtgttga aaattgagtt gataaataat cttcgatcag ttcatgtttg taacccaatg
    12001 agaagataaa tttttctact ccctggcttc tcaggtaatt gataacatga aaaaggaatg
    12061 gtcgtccggc aacaggtgcc atgcatttcg gaagatcagg tacagattcc cttaatcttg
    12121 ttcctaagcc accagctaat attattgcct ctttgatcat gcaggttgtt ataagtctac
    12181 cattatgtgg tgatacagag aggtgcaaag tatttttctt ccaccagctg acaattgata
    12241 tgctcgatag taatatgact ttcttgaatt cttggtgtat
//
LOCUS       DAORTI010000107        12257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5219_length_12257_cov_7.161367, whole genome shotgun sequence.
ACCESSION   DAORTI010000107 DAORTI010000000
VERSION     DAORTI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_12257_cov_7.161367"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1331)
                     /locus_tag="PLO70_12515"
     CDS             complement(258..1331)
                     /locus_tag="PLO70_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ75334.1"
                     /translation="MSKKIQLTVPTPCHENWDAMTPVEKGKFCGSCQKQVMDFTSMSD
                     REIAQFFKKPSTGSVCGRFMNDQLDREMEIPKKRIPWLKYFFQIALPAFFVSKVSAQK
                     VMGKSAAPDGRDTSKVRITTEMRTLGMVLPTCIKPVTGDTLITAVKGEVTSKPVENNS
                     QVVGVVMDETGMKLPGVTVRIKGTRTGVAADRYGRFRLMAKTDDVIIVSGAGLETTEI
                     RVGQNKDLSIVVKRLLITGGEVVVVAGMVSVKRTKKIKKTIPLLTQIYKDTAAKFFKV
                     YPNPVPAGSNISIEWKQTGEGYYSLQLFNQAGQSVHQQEIWIDAEARLLNIDFPAVAA
                     GNYFLSFISKKTGRKCTEKIIIK"
     gene            complement(1341..2384)
                     /locus_tag="PLO70_12520"
     CDS             complement(1341..2384)
                     /locus_tag="PLO70_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQZ75335.1"
                     /translation="MRKICTLLNLCITTVCLSQAITTAKVITSNNLTTTTIDSSINLQ
                     KGLTGKVTGLNVQTVNKDAGGARITLRCGRTHFPNEAPLLIIDGVVQELSILQAINPG
                     AIKSVDVLKSAVATALYGPEGVYGAIYVTTKNGNCRTFVFKDFLDGSKIAGATVSFIS
                     ADKKDILMYAANDSGIVVADKLKPAVQYEIKISAVGYKNICVDYINKHTGLYSEILLS
                     RKEKVCDEVVITYFGHTIRCYGSCCIARIRIKGDSINKKIKKQGFKIFPNPVRKGGVI
                     NIEIENSKEERLTVKMLALDGRVLLDKSVQVLKGSGRFQLATDTRWAAGVYIIHLAYA
                     NGKILASEKVIIQ"
     gene            complement(2502..4481)
                     /locus_tag="PLO70_12525"
     CDS             complement(2502..4481)
                     /locus_tag="PLO70_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HQZ75336.1"
                     /translation="MKRILLLIVPVILILLAFRPITVITVTGSITDDKGSPVAGASVF
                     VKGTKSGTSSKTDGTYRIVVTKANDVLVFSAVGMQTQEVKVKGRSIINVSLTMATNTM
                     SEVVVVGYGRAQKKEMTGSVATVHPSQLYGSRGYDQPLQGKVAGIYIQGEQGYYTNDK
                     PVEDFEREGYDNITENKFLKVADNPLSTFSIDVDAASYSNIRRFLNNGQLPPAGAVRI
                     EEMVNYFTYEYPQPENNEPFSINTEIAEAPWNKNHKLVLIGLQGKKISTENLPASNLV
                     FLIDVSGSMSSQNKLPLVKASMKMLVDQLREEDKVAIVVYAGAAGLVLPPTTGENKSE
                     IKEAIDKLESGGSTAGGAGIKLAYKTAKEYFVKGGNNRVILCTDGDFNVGESSDDAME
                     RLIEAERKSGVFLTVLGYGMGNYQDAKMQKLADKGNGNHAYIDGITEAKKVLVNEFGG
                     TLFTIAKDVKLQIEFNPAKVQGYRLIGYENRMLAKEDFNDDKKDAGELGSGHTVTALY
                     EVIPVGVNSSFLKNVDSLKYQQNTERLSKSSNTDEILTVKFRYKAPDGDVSKLIVHPV
                     NDKQIPIARTSDNFRFAASVAQFGMLLRNSEFKSNASYNDVISLARKAKGYDEEGYRS
                     EFIRLVESAQMLAKGKAAPKDDADTEDGEPMPVNK"
     gene            4566..5156
                     /locus_tag="PLO70_12530"
     CDS             4566..5156
                     /locus_tag="PLO70_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ75337.1"
                     /translation="MTFIRNISHSSLTDKELVALYKESGDMAILGELYQRYMELVYGV
                     CLKYFKEPERSKDSVMLIFEELVTKLKKHEVENFRAWLHQLAKNHCLMQLRTPKNMKT
                     VEFKVELVQSEENVHLNGVLEKEENFRKLEYCLGTLTSEQKESIKLFYLEGKSYNEIV
                     DITGIEWNQVRSLIQNGRRNLKICMDKAESGIGNRE"
     gene            5196..6605
                     /locus_tag="PLO70_12535"
     CDS             5196..6605
                     /locus_tag="PLO70_12535"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ75338.1"
                     /translation="MANDNNIKKFTAIDIEKYHKGLLSPKEMHDLEKAALEDPFLADA
                     LEGYAVTGVNAEADIAELKKRLAEKTGQAKVIPLSGGNKNNIPWLRAAAMVVLLAGAA
                     VLAYVFVFDKKSDEIAKADPAKTEQVKKADSVVMPATVDKTAAAPVATNTGEQTQPTA
                     ATKNDTKIPAHTTGETSNDIASASGKASTDLNKPTGEEPAIVPGENVVRTESMKEKKS
                     EAEEEVKARTAAVAKKDTDKNLAAISATQENNEQKVNRAVTMNRQAADQYRNTNIFRG
                     RVTDASNTGVPFANVTNVEDNVGTYADAKGYFNLTSPDTLLNVQVRSIGFENSNIQLR
                     NNVPTNQVVMQDDRRSLSEVVISNQKPNAAARSQNNNVKLEEPEPADGWDNYDAYLAN
                     NLNMPEDFKSKESNTAMVQVSFEVDRNGEPINIRVEKSLCKKCDKEAIRLIKEGPKWK
                     RNAKNGRTTVTIPFNQLFH"
     gene            6672..7505
                     /locus_tag="PLO70_12540"
     CDS             6672..7505
                     /locus_tag="PLO70_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ75339.1"
                     /translation="MKKLLFTSIAAVSLLMAEAQLKTPAPSTTQTIKQDFGLGTIELS
                     YSRPGMKGRKIFGDLVPFGNVWRTGANNATLITFSDAVTIGDKKVPAGKYGLLSIPDK
                     NNWILIITKQLDVTSPAAYKQESDVARVEVKTMKMGEKVETFTMQFADVKPTSCELHI
                     MWENTAVALPITTDVDGKVMAQIDNLMNKDNRPYFNAAMYYMDNGKDLNQALTWFDKA
                     VELNPSAFWIHHQRANCLAKLGKKAEAKAAAEKSKELAAAAKNGDYVTLNEKLLAELN
                     K"
     gene            complement(7543..9279)
                     /locus_tag="PLO70_12545"
     CDS             complement(7543..9279)
                     /locus_tag="PLO70_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQZ75340.1"
                     /translation="MSQLDWIVLIVTLLAIISYGLYKSRTSRNLEGYFLSNRSMPWGL
                     VLLSIMGTQASAITFLSAPGQAYTDGMRFVQYYFGLPLAMIVICIFFVPIFSKLKVFT
                     AYEYLENRFDGKTRTLTSFLFLLQRGLSTGISVFAPSIILSSLFGWNIYYTNILMGGL
                     LIIYTMSGGAKAVAYTQQLQLLIIFTGMFLAGYMVVNMLPANVSFADALQVSGKLGKL
                     NVITTGFENGKFNWSDQYNLLSGLIGGFFLALSYFGTDQSQVGRYLTAKSLRESRLGL
                     LMNGLVKVPMQFAILLIGALVFTFYQFNTAPIFFNEVQVKKLEQSAYKDSFVLVQQQY
                     EAITEKKHQMVIAYSTATDNDDKLAEESSLASLRSLQKSADSLRGKVKAWLNSKQVGG
                     DGNDTNYIFLRFVVDHLPVGLVGLLIAIIFLASWGSIAAAINSLSSSTMVDFHRRFTK
                     KEDTGETEYRLSKWYTLAWGVFCIIVAMFTYNIGNSLIEAVNVLGSLFYGVILGVFLV
                     AFFIKRITNGSVVFWAALLAELLVLTVFILTKTGVFKMGFLWLNPIGAFGVIFFALLL
                     ERIYKQKESPAV"
     gene            complement(9406..9582)
                     /locus_tag="PLO70_12550"
     CDS             complement(9406..9582)
                     /locus_tag="PLO70_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75341.1"
                     /translation="MATNSKPSVRRGEMAFIFAIIIGLVIGILIKKVRIGIIIGLILG
                     SLIVFTGWLRTTRK"
     gene            complement(9582..12077)
                     /locus_tag="PLO70_12555"
     CDS             complement(9582..12077)
                     /locus_tag="PLO70_12555"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQZ75342.1"
                     /translation="MKRKIVTVFAFPLLFTARLLAQSPQSWSSADMYQALRKLNVLGS
                     VLYVAAHPDDENTRLIAYLSKDKLYRTGYLSMTRGDGGQNLIGEEQGIELGMIRTQEL
                     LAARRIDGGEQFFTRAYDFGYSKTPKETFNKWDKEKILSDVVWVIRKFQPDVIINRFP
                     TTGEGGHGHHTASGILANEAYEAAADPGRFPEQLKFVSVWKAKRVLWNTFNFGGTNTT
                     RDDQFKIDVGGYNPLLGKSYGEIAAESRSQHKSQGFGVPASRGESMEFFKATQGDQPV
                     NDLMEGVELSWKRVAGAEAIGKMIDSLTSTFDLLHPERSVEGLVKLYTAINNLPDSYW
                     KTQKAGEVYQLIAQCSGLFLDVTTTEQFAVQTDSIRLNFSFNNRLGTEAVLQKVQVDN
                     FDSAFLRTLEKNKNINFSKTLFVPSTKPVTQPYWLVNKMAEGYFNVTDQLSIGQPDVD
                     PAYAAFIQVKIFGERFTFMRTVRYKFTDPVKGELYQPLVVIPPVIITPVEDMKIVTGD
                     KINLKGSVTVRGMKKGFSGTMQAISDTTGKALKNVFIKNNTISINTKNQLTEVDYTGE
                     AEKKNENIRFAVSSVEGNHIADDRHEIRYDHIPFINYFHKAVVAVKYIDLKTYNKRIG
                     YIVGAGGKVPESLEQMGYEVILLTDKELSRNNLGQYDAIITGVRAYNTHDWMNKHYEK
                     LMKYVNEGGNLIVQYNTSNQIGPVRAKIGPYNFNITRTRVTDENAAVTLLKPEHPVFN
                     FPNKISGDDFKGWIQERSIYHATDTSGKFEKLIGMADPGEKSDDGSLLVAKYGKGWFT
                     YTGIVFFRELPAGVPGAYRLLANIIALNKKKGF"
     gene            complement(12077..>12257)
                     /locus_tag="PLO70_12560"
     CDS             complement(12077..>12257)
                     /locus_tag="PLO70_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75343.1"
                     /translation="KQICHIEFIEMRTNLFANHCYISTMLNMTLIEAFEAASSFIWKV
                     QLLFEQTLYSLIKPM"
BASE COUNT         3629 a         2802 c         2312 g         3514 t
ORIGIN      
        1 acggttttca gaacttagaa agtcattccg gccctgagcc ggaatctttt tctaaaataa
       61 acataccatc ccgatagtta tcaaaccgaa gtgatggtac cctgattgct caactttatc
      121 gggaatgtat acgaaaaaag ccggatcagt ttcatacggt tttcagaact tagaaagtca
      181 ttccggccct gagccggaat ctttttctaa taataaactt atcatcccga tagttatcag
      241 caggagatga tatttaatta ttttataatt attttttctg tacacttcct tcctgttttc
      301 ttactgatga atgacaggaa ataattcccg gctgcaacag ctggaaaatc aatattcagc
      361 agtcttgctt ccgcatcaat ccatatttcc tgctgatgaa cagactgacc cgcctgattg
      421 aatagttgca gtgaataata cccttctccg gtttgtttcc attctatgga aatatttgag
      481 ccagccggaa caggattggg atacacttta aagaatttag cagcggtgtc tttatatatt
      541 tgtgtcaaca agggaattgt tttctttatt ttctttgttc tttttaccga aaccatcccg
      601 gctactacta ccacttcccc acccgttata agcaaccttt tcactacaat tgacaggtcc
      661 ttattttgcc cgactcttat ttctgttgtt tcaagcccgg ctcctgaaac gattatcaca
      721 tcatcagttt tagccataag tctgaaccgg ccatacctat cagctgctac acccgtcctt
      781 gtacctttga ttcttacggt tacaccgggt agcttcattc ctgtttcatc cattactacg
      841 ccaacaacct ggctgttatt ctcaacaggc ttacttgtca cttcaccttt tactgcagta
      901 ataagtgtat caccggtaac aggcttgatg caggtgggca gtaccattcc caatgtccgc
      961 atttcagtgg ttattctcac tttcgatgta tctctcccat ctggtgcagc ggacttgccc
     1021 atcactttct gtgcggaaac ctttgatacg aaaaatgccg gtaatgcgat ctggaaaaaa
     1081 tattttaacc acggtattct tttcttcggt atctccattt ctctatccag ctggtcgttc
     1141 ataaaacgtc cgcataccga accggtggat ggttttttaa agaactgtgc tatctcccgg
     1201 tcactcatag aagtgaaatc cattacctgc ttctggcagg aaccgcaaaa ttttcctttt
     1261 tccacaggtg tcatagcgtc ccagttttcg tggcagggag ttggtacggt aagctgtatt
     1321 ttcttgctca taatagtgtt ttactggata attacttttt cagatgcaag aatcttcccg
     1381 tttgcataag ccaggtgaat aatatatact cctgctgccc atcgggtatc ggtagccaat
     1441 tgaaaacggc cggaaccttt taacacctga actgatttat ccagcaacac tctgccatcc
     1501 aatgccaaca tttttacagt aagcctttcc tccttactgt tttcgatttc aatatttatt
     1561 accccgcctt ttcgaaccgg gttgggaaag attttgaacc cctgtttttt tatcttttta
     1621 tttattgaat ctcctttaat ccttattctt gctatacaac aactaccata acatctgatc
     1681 gtatgtccaa aatatgtaat aactacttca tcacacactt tctctttcct ggataataat
     1741 atctcactat acaaaccggt atgtttattt atgtaatcga cgcatatgtt tttgtatcct
     1801 acagcagaaa tttttatttc atattgaaca gccggcttca atttatcagc aaccactatc
     1861 ccgctatcat tggctgcata catcagtata tctttcttat cggcagaaat aaaggaaaca
     1921 gttgccccgg caatcttact gccatcaaga aaatccttaa aaacaaaggt tctgcaattc
     1981 ccattctttg tggtcacata tatcgcccca tatacacctt ccggaccata taaggctgtt
     2041 gccactgcac tttttagaac atcaacactt ttaattgcac caggattaat cgcttgcaaa
     2101 atagaaagct cctgcacaac gccatctatg ataagcagtg gggcctcgtt cggaaaatgt
     2161 gttctgccgc acctgagtgt tatccttgca cctccggcat ctttattcac agtttgtaca
     2221 ttcagaccgg ttactttacc ggttaatcct ttttgtaaat taatactact atcaatcgta
     2281 gttgtagtga gattatttga cgtaataact tttgccgtag taatagcctg tgacaggcaa
     2341 acagtagtaa tacataaatt aaggagtgta catatttttc tcataagctt agtttttagg
     2401 tcgcacatcg gcaggctcag tgcaacaggt ttaataaaaa ggggccggta gaaaccagcc
     2461 ccgaatcacc aaccggctgt taaatgatta gctatttatt tttatttatt gacaggcatt
     2521 ggctcgccat cttctgtatc agcatcatct ttgggcgctg cctttccttt tgccagcatt
     2581 tgggcactct ccaccagcct gataaattca gaacggtagc cttcttcatc atagcctttg
     2641 gctttacggg ccaggctaat tacatcatta taggaagcat tagatttaaa ctcagagtta
     2701 cggagcagca taccaaactg tgcgactgat gctgcaaagc ggaaattgtc ggatgttctg
     2761 gcaatgggga tttgtttatc attaaccggg tgtacaatca gtttgctaac atccccatca
     2821 ggagctttat agcgaaactt taccgttaat atttcatccg tattagacga cttactcagc
     2881 cgttcagtat tctgctggta tttcagggaa tccacatttt taaggaaact gctgttcact
     2941 cccaccggga tcacttcata caacgcagta acagtatgtc cgctgccgag ttcaccggca
     3001 tcttttttat catcattaaa atcttctttg gccagcatcc tgttttcata accgatcagg
     3061 cggtatccct gaactttggc aggattaaat tcgatctgca acttcacatc tttggcaata
     3121 gtaaaaagtg taccgccaaa ttcattcacc aatactttct ttgcttcggt gattccatct
     3181 atgtacgcat ggttcccatt acccttatca gccagctttt gcatcttagc atcctggtaa
     3241 ttacccattc cataacctaa caccgttaaa aacacaccac tttttctttc agcttcgatc
     3301 aacctttcca ttgcatcatc actgctttct ccaacattga agtcaccatc cgtacaaagt
     3361 atcacccggt tgttacctcc ttttacaaaa tattcttttg ctgttttata agccagtttg
     3421 ataccagcac ctccggcagt agacccgcct gattccagct tgtctatcgc ctcttttatt
     3481 tcactcttat tctctccagt ggttggcggt aataccaacc ctgcagcgcc tgcatatact
     3541 acaatcgcaa ctttatcctc ttccctcaat tggtccacca gcattttcat agaagccttc
     3601 accaatggaa gcttgttctg tgaactcata gaacccgaaa catcgatcag gaaaaccagg
     3661 ttggaagcag gcaaattttc tgtagagatc tttttgccct gcaaaccaat taaaacaagc
     3721 ttatggtttt tattccacgg cgcctccgct atttcagtat taatggaaaa tggctcgttg
     3781 ttttccggtt gcggatattc atatgtaaaa tagttgacca tctcttcaat acgtactgca
     3841 cctgccgggg gtaactgacc attattcaaa aaacggcgaa tattactgta tgaagcggca
     3901 tccacatcaa tagaaaaagt agacaatggg ttatcagcca cttttaaaaa tttattttct
     3961 gttatattat catagccttc ccgttcaaag tcctctactg gtttatcatt tgtatagtac
     4021 ccctgttctc cctgtatata gataccagca actttcccct gtaatggctg atcgtagcct
     4081 cttgaaccat acagctgtga tggatgaacc gttgccacac ttcctgtcat ttcttttttt
     4141 tgggccctgc catatcccac aacaacaact tcactcattg tatttgttgc catggttaat
     4201 gaaacattaa taatagatcg gccttttact tttacttcct gtgtttgcat tcctacggct
     4261 gaaaacacaa gcacatcatt cgcctttgtc actacaatcc tgtacgtacc atcggttttg
     4321 gaactggttc ctgattttgt acccttgaca aaaacagaag caccggcaac aggactgcct
     4381 ttatcatctg ttattgatcc ggtaacagtt atgacagtga ttggccggaa tgccagcagg
     4441 atcaaaatga ccggcactat gagaaggagt attcgtttca taaaaaagta tttacatacc
     4501 ctgatgcggg tatttttaga attgcataag gggctataaa aattttattc gctaatttta
     4561 agccgttgac ttttatcagg aacatatctc actcctccct aaccgataaa gagctggtgg
     4621 ctctgtataa agagtcgggc gacatggcca tactgggcga actctaccag cggtacatgg
     4681 aactggtcta tggtgtttgc cttaagtatt ttaaagaacc ggaacggtcc aaagacagcg
     4741 tgatgctgat ctttgaggaa ctggtaacca aattgaagaa gcatgaagtg gaaaatttca
     4801 gggcctggct gcaccagctg gccaaaaatc actgcctgat gcagctacgg acaccaaaaa
     4861 acatgaaaac cgttgagttt aaggtggaac ttgtgcaatc ggaggaaaat gtgcatctga
     4921 atggtgtgct ggaaaaagag gaaaatttca ggaaactgga atactgcctc ggcaccctga
     4981 caagcgagca gaaagagtcc attaaactgt tctacctgga aggcaaatcg tataatgaaa
     5041 tagtggatat cactggtatt gaatggaacc aggtacggag cctgatacag aatggcagac
     5101 gaaatctgaa gatatgtatg gacaaagctg agtcaggaat cgggaacagg gagtaagaag
     5161 tatgaacgaa aaaaataaga actttgtaat gccgtatggc aaacgacaat aacataaaaa
     5221 aatttaccgc tattgatatt gaaaaatatc ataaaggcct gctttcccca aaagaaatgc
     5281 atgacctgga aaaagcagca ctggaagatc ctttcctggc cgatgcactg gaagggtatg
     5341 cggtgactgg cgtaaatgca gaagcagata ttgcagaact gaaaaaaaga ttggctgaaa
     5401 aaacaggaca agccaaagtg atcccgttga gtggcggcaa taaaaataat attccctggt
     5461 taagagcagc agcgatggta gtgctgttgg ccggcgctgc tgtgttagct tatgtttttg
     5521 tatttgataa aaaatccgat gaaatagcca aagcagaccc tgctaaaact gaacaggtaa
     5581 aaaaagcgga ttcagttgtg atgccggcaa ctgttgataa aactgccgct gcacctgtcg
     5641 ccactaatac aggtgagcaa acccagccaa ctgcagctac taaaaatgat acaaagattc
     5701 cggctcatac taccggggaa acaagcaatg atattgcctc cgcatccgga aaagcctcta
     5761 ctgacctgaa caaacctact ggggaagaac cggcaattgt tcccggcgaa aatgtggttc
     5821 ggacagaaag catgaaggaa aaaaaatcgg aagctgagga ggaagtgaaa gcaaggactg
     5881 cggcggtggc taaaaaagat accgataaaa atttggcagc tattagtgca acacaggaaa
     5941 acaatgaaca aaaagtaaac agggcggtaa ccatgaaccg ccaggctgca gatcagtacc
     6001 gcaatacaaa tattttccgt ggccgtgtta cagatgcctc caataccggt gtgccttttg
     6061 ccaatgttac caatgtagaa gataatgtag gcacttatgc tgatgcaaaa ggttatttca
     6121 atttaacttc cccggataca ttattaaatg tacaggtacg atcaataggt tttgaaaata
     6181 gtaatataca gctgcgcaat aatgttccca ctaaccaggt agtgatgcag gatgaccgaa
     6241 gaagtttgtc tgaagtagtg atcagtaatc aaaagcccaa tgccgcagcc cgttcacaaa
     6301 ataataatgt taagctggaa gaacccgaac ctgctgatgg atgggacaat tatgatgcat
     6361 acctggccaa caacctgaac atgcccgaag attttaaatc aaaagaaagc aataccgcga
     6421 tggtacaggt ttcttttgaa gtggacagga atggtgaacc tattaatatc agggtcgaaa
     6481 aatccctctg caaaaagtgt gataaagaag caatccgcct cattaaagaa ggtccgaagt
     6541 ggaagcgcaa tgccaaaaac ggaaggacca ccgttaccat tccgttcaac cagctgtttc
     6601 actaacagct aaaactgtct tcccggtatt caatccatac gcttaccttc gcatttcaaa
     6661 aattgcataa aatgaaaaag ttattgttca cctctatcgc ggctgtaagc ttattgatgg
     6721 ctgaagccca gttaaaaaca ccggcgccaa gtaccaccca aaccattaaa caggattttg
     6781 gtcttggcac tattgaactt tcttattccc gcccgggtat gaaaggaaga aaaatatttg
     6841 gggacctcgt accatttggc aatgtatggc gtacaggagc taataatgcc acccttatca
     6901 cattcagtga tgctgtaact atcggtgaca aaaaagtacc tgccggaaaa tatggcctgt
     6961 taagtatccc agataaaaac aactggatat tgatcatcac caaacaattg gatgtaacca
     7021 gcccggctgc ttacaaacag gaaagtgatg tggcaagagt agaagtgaaa accatgaaaa
     7081 tgggtgaaaa agtagaaact tttaccatgc aattcgccga tgtaaaacca acaagctgtg
     7141 aactacacat catgtgggaa aatacggcag tagcgctgcc cattacaact gatgtagatg
     7201 gaaaagtaat ggcgcagata gataacctga tgaataagga taaccgtcct tatttcaatg
     7261 ctgctatgta ttatatggat aatggcaaag atctgaacca ggcacttaca tggtttgaca
     7321 aagctgtaga acttaatcca tcagctttct ggattcatca ccagagagca aattgcctgg
     7381 ccaagctggg caaaaaagca gaagcaaaag ccgctgcaga aaaatcaaaa gaactggcag
     7441 ctgcagccaa gaatggcgac tatgtaacgc tgaacgaaaa attattagca gagttgaata
     7501 aataattgct ttgcaatatt cttattccca ggagagccct gcttatacag cagggctttc
     7561 tttttgtttg tatattcttt caagcaataa agcaaagaaa ataaccccaa aagccccaat
     7621 aggattcagc cagagaaagc ccattttaaa aacacccgtc ttcgtcaaaa tgaaaactgt
     7681 cagtaccagt aattctgcga gcagtgctgc ccaaaaaaca acactaccat tggtaattct
     7741 tttgataaaa aaggcaacga gaaatacgcc caaaataaca ccataaaaaa gtgaacccaa
     7801 tacattgacg gcttctatca ggctattacc aatattgtag gtaaacatgg ccacgatgat
     7861 acaaaacaca ccccatgcca gggtgtacca tttagacaac ctgtactcgg tctctccagt
     7921 atcttctttt tttgtaaatc gccggtgaaa gtcaaccata gtggaagagg aaagtgagtt
     7981 gatagccgca gcaatacttc cccaggaagc aagaaaaatg atggctatta acaggcctac
     8041 caatccaaca ggtaaatgat ctaccacaaa ccggagaaag atgtagttgg tatcgttacc
     8101 atcaccaccc acctgtttgc tgttcaacca tgcttttact tttccccgca ggctgtctgc
     8161 actcttttgc aggctgcgca aacttgctaa tgatgattct tctgccagct tatcgtcatt
     8221 atctgtggct gttgagtaag caatcaccat ttgatgcttc ttttccgtaa tggcctcata
     8281 ctgttgctgc accagcacaa aggaatcctt gtaggcagac tgctccagtt tctttacctg
     8341 cacctcgtta aaaaatatag gcgcagtatt gaattgataa aaagtaaata ccaatgcacc
     8401 aatcaataag atagcaaact gcatgggcac ttttaccagg ccattcatca acaaaccaag
     8461 ccggctttcc cgtaatgatt ttgcagtaag ataccggccc acctggctct ggtcggtacc
     8521 aaaataagat agtgccagga aaaaaccgcc gatcagacca ctgagtaaat tatactgatc
     8581 actccagttg aacttaccat tctcaaatcc ggttgtgatc acattcagct ttcccaattt
     8641 accacttacc tgtaatgcat ctgcaaaact cacattagcc ggcaacatat tcactaccat
     8701 atacccggcc aggaacattc ctgtgaaaat gataagcagt tgcagttgct gtgtataggc
     8761 caccgctttg gctccgccac tcatcgtata aataataagc aacccgccca tcagtatatt
     8821 ggtataatag atgttccagc cgaataatga ggaaagaatg atagaaggtg caaatacact
     8881 gataccggtt gacagacctc tttgcaaaag aaataaaaat gatgtcagtg ttcttgtttt
     8941 tccatcaaac cggttttcca ggtattcata cgctgtaaac acttttagtt tactgaaaat
     9001 gggcacgaaa aaaatacaga taacgatcat ggccaggggc agaccaaaat agtattgcac
     9061 aaaccgcatc ccatcggtgt aagcctgtcc cggcgcagat aaaaaagtaa tcgcacttgc
     9121 ctgtgtaccc atgatactca ataacaccag gccccagggc atactgcggt tgctgagaaa
     9181 ataaccttca agattacggg aagtgcggct tttatacagg ccataagaaa tgatggccag
     9241 cagggtaaca atcagtacta tccaatcgag ctggctcatg caaatcgttt tgtgaacaaa
     9301 tagaacaata caataagcaa taccaggaaa aggatgacga acaaatacca gcctttccag
     9361 cttttaaata atggcgcctt atcgttatcc ggtgtttgca tctggttatt tccgggtggt
     9421 tcgcaaccaa cctgtaaaga caattaatga acctaaaata agtcctatta ttatacctat
     9481 cctcactttc tttatcagga tgccaattac caatccgatt attattgcaa agatgaaagc
     9541 catctctcct cttcttacag aaggtttaga attagttgcc atcaaaatcc ttttttctta
     9601 ttcagtgcaa ttatattggc cagcagcctg tatgcaccgg gaacaccggc aggcagttcc
     9661 ctgaaaaata cgataccggt ataggtaaac caacctttcc catatttcgc cacgagcaaa
     9721 cttccatcat cagacttttc tcccggatca gccataccaa tcagtttctc aaattttcct
     9781 gatgtatctg ttgcatgata tatacttctt tcctgtatcc accctttaaa atcatcaccg
     9841 gagatcttat taggaaaatt gaaaacggga tgttccggtt ttaacaaggt aacagctgca
     9901 ttctcatctg taacccttgt acgggtaata ttaaaattat aaggccctat tttagctcgt
     9961 accgggccga tctggttact ggtattatac tgaacgatca ggtttcctcc ttcattcaca
    10021 tacttcatca gcttttcata atgcttgttc atccaatcat gagtgttgta agcccttaca
    10081 cccgtaatga tcgcatcata ttgtcccagg ttatttcttg aaagttcttt atctgtcagc
    10141 agtatcactt catagcccat ttgctcaagc gactccggga ccttgcctcc ggcaccaacg
    10201 atataaccaa tccttttgtt atacgttttt aagtcaatat attttactgc aacaactgct
    10261 ttatggaagt aattaataaa aggaatatga tcatacctga tctcatgcct gtcatcagct
    10321 atatgattcc cttcgactga tgaaacagca aaacgtatat tctcattctt tttttctgcc
    10381 tcgccggtat aatcaacttc tgtaagctga ttttttgtat tgatactgat cgtattgttt
    10441 ttgatgaata cattcttcaa ggcttttcca gtagtatctg aaatggcctg catcgtaccg
    10501 gagaaacctt tcttcatgcc tcttactgtg acagaccctt tcaggtttat tttatcgcca
    10561 gttacaattt tcatatcctc cacgggagtg ataatgacgg gcgggataac taccagtggc
    10621 tggtacaact ctccttttac cgggtcggtg aatttatacc ggactgttcg cataaaagtg
    10681 aaccgttctc caaatatttt tacctggata aaagcagcat aagccggatc aacatccggc
    10741 tgaccaatag acaactgatc tgtcacatta aaatatcctt ctgccatttt attcaccagc
    10801 cagtaaggct gggtaacagg tttagtggac ggaacaaata aagtctttga aaaattaatg
    10861 ttcttatttt tctccaatgt tcttaaaaaa gcagaatcaa aattatcaac ctgtactttt
    10921 tgaagcaccg cctctgttcc caggcgatta ttgaaagaaa aattcaaccg gatagagtca
    10981 gtctgcacgg caaactgttc cgtagtagta acatctaaaa acaaaccact gcattgtgca
    11041 atcagctggt atacttcacc cgccttttgt gttttccagt aactatcagg aagattatta
    11101 attgcggtat ataatttaac caggccctcc accgatcttt caggatgcag gagatcaaat
    11161 gttgaagtta ggctgtcaat catttttcca attgcctcag cacctgcaac tcttttccat
    11221 gacagctcca ctccttccat taaatcattt accggttggt ccccttgtgt ggccttaaag
    11281 aattccattg attcacccct ggaagccggt accccaaagc cctggctttt atgctggctg
    11341 cggctttcag cagcgatctc tccatagctt tttcccaata atggattata cccacctaca
    11401 tcaatcttaa actgatcatc tcttgttgta ttggtcccgc caaagttgaa cgtattccat
    11461 aatacccttt tggccttcca taccgaaaca aattttaatt gctcgggaaa acgaccggga
    11521 tctgcagcag cctcatatgc ttcgttcgcc aggattccgg acgccgtgtg atgtccatgt
    11581 ccgccttcac cagtagtagg aaagcggtta ataataacat ccggctgaaa ctttcgtatc
    11641 acccatacca catcactcaa tattttttcc ttgtcccatt tgttaaatgt ttcctttggt
    11701 gttttggaat aaccaaaatc ataagccctg gtaaaaaact gttctcctcc atcaattctc
    11761 ctggcagcta acagctcctg ggttctgatc atccccaact cgataccctg ttcttcaccg
    11821 ataaggtttt gcccaccatc accccttgtc atcgataagt agccggtgcg gtataatttg
    11881 tccttggata aataagcaat caatcttgtg ttctcatcat ccggatgtgc cgctacatat
    11941 aataccgacc caagtacatt gagcttgcgg agtgcctggt acatatcagc ggaagaccag
    12001 ctttgcggag attgcgccaa tagccgggct gtaaataaaa gagggaaagc gaacactgtc
    12061 acaatttttc ttttcattac attggtttaa taagcgaata tagtgtttgt tcaaaaagaa
    12121 gctgaacctt ccatataaaa gaagaggctg cctcaaaagc ctcaatcagt gtcatattga
    12181 gcattgtcga aatataacaa tgattggcaa acaaatttgt acgcatttcg ataaactcaa
    12241 tgtgacaaat ttgtttg
//
LOCUS       DAORTI010000108        12132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5314_length_12132_cov_5.709034, whole genome shotgun sequence.
ACCESSION   DAORTI010000108 DAORTI010000000
VERSION     DAORTI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_12132_cov_5.709034"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..2087)
                     /locus_tag="PLO70_12565"
     CDS             complement(330..2087)
                     /locus_tag="PLO70_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQZ75344.1"
                     /translation="MNINKYYTQQLDVFKKSITSFKTSCQKKETTTDLKARFYQSRLT
                     YKKLSVLTDYFNQYETKILNGPPIDHIESEVKDRIIPPQGFQAIEDLLFSGKWSNSSY
                     PEVINLLDQMLQIISKLENEPDRIYKFKDELVWDAVRSACVQLITTGITGFDSPAANY
                     SLPEATASLAGIKTLLSFFKPLIEKNKVNSYVQLNALLDKSVSYLQRNNNFNEFDRLT
                     FITQYINPFYKLLAQTRIESVIGIPGGRNSINFNSLSIFEADALNINFYSPPEEYWVT
                     PERIRLGKLLFSDPILSGTRTRSCASCHLPEKAFTDGFAKPFALDGITLLARNTPTLY
                     NSGFQTKQFFDSRADILENQLGDVVHNVEEMNGSLKNSVSELKKSDDYTKFFQAAYQS
                     ERDPINSFTIANSISCYVRSLQSLNSRFDKYMRGDYKQLSLAEKKGFNLFAGKAKCAT
                     CHFIPLFNGVVPPFFAETESEVLGVPKSKDKSMAELDSDPGKFNFTNSIVHKYAFKTP
                     TLRNIALTAPYMHNGVYDTLEEVMEFYNNGGGKGLKIAPENQTLPFYKLNLSKNEITH
                     IIAFMKTLTDTSIIYTKYK"
     gene            2361..5516
                     /locus_tag="PLO70_12570"
     CDS             2361..5516
                     /locus_tag="PLO70_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ75345.1"
                     /translation="MKPLSSTLKALILYLIITSVAFNSFSQTTIVAYGSSWKYLDNNT
                     RPANWETSAFNDAAWASGNGQLGYGDGDEATVVNHGGCSPIATCGPKYITTYFRKSIN
                     ITDPSAYVNFTLNVLRDDGIVIYINGTERYSNNMPAGRLHSTLATAAASDDGATPQTA
                     TLSSSFFSAGTNVIAVEIHQNSTTSSDITFDLELLGNAAPSGLINYGSTWKYLDNNTR
                     PAGWETIAFNDVAWSSGLSEFGYGDGGEATCVASGGGGTLCTPTGNKYITTYFRKTFN
                     VTGLNTFNSFTLNIIRDDGVVVYINGTEVARNNMPGGTPAHSTLASAAIGGTDETTPI
                     TFSLSPCAFVEGTNTIAVEMHQNDVTSSDLSFNLELNGIVGGGTPTLTRGPYLQVASE
                     TAITFRWRTSTACYGRVSVGPSNGTYTTATVDETCPTTEHIIRVTGLASDTKYFYQIS
                     ATDGTVFQGAADNFFRTNPPANTTRKIRIAAFGDCGRNNATYQDNNLANYQNYLSVNG
                     IDAPDAWILMGDNAYNSGTDAEYTTNFFNIYGNNILKNHKLYPSPGNHDYGNSSTNKP
                     SRSMPYHTNFSVPQNGEAGGVASNKQNFYSFDVGNIHFLSLDSYGTESDLTDMQTAGA
                     SALKTWLDADLAANTKKWVIAYWHHPPYTKSSHNSDSESDLVNIRQNFITFLETRGVD
                     MIICGHSHAYERGYLLRNFTGAWTSFNPATHAVSTSSATYTSNSTCPYVYNTTPANHG
                     TVYVVAGSAGASGGTNAGFGANAMPFAVNDAGLFYFEVEDNRLDAKMLRRNGTIFDQF
                     TIMKDVNQVTNYNIPNGSSQTLTASWPGNYSWSTSATTRAVNVTPPNNAVTNYTVSDD
                     FGCITDQFSVTTSGTLPVSLLTYDVRLTGNKVNTTWSTSTEVNNSFFSIERSSNGRDF
                     IVIGNVNGAGNSTTNRTYAFTDAQPLLGTSYYRLSQTNFDGQREYMGVRRIENTSLKE
                     FEVKTISGYAGKLILQINSSVDGVYQLSIVDMTGRKIKNEVLGINAGISTKEIYLNPG
                     VYIWEVRNSKGDAMFQKVIVQ"
     gene            5545..10389
                     /locus_tag="PLO70_12575"
     CDS             5545..10389
                     /locus_tag="PLO70_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75346.1"
                     /translation="MKKIYVIVVLLLLCLVQQGYSQVLRSMTPRYNNASVKGNIVFVS
                     NNIITSSGAITTEAPPGGTATNNGHTGLNVDVDGTPPTSYIAFGSNWKYLDNNTRPVG
                     WETVAYVDAAWAAGNGQLGYGDGDETTIVNHGGCAPIATCGPKYITTYFRKTINIPNP
                     ATHSNFTLNVRRDDGVAVYVNGVEVYRNNMPAGVLAHGTLASAAAADDGNTVQTATIA
                     SGAFIAGNNVIAVEVHQNNNTSSDLSFDLELLGNPITTVTVVPYGANWKYLANNTRPA
                     GWETVPYVDAAWPAGNAEFGYGDGDEATCVPSGGGGTLCAPTGNKWITTYFRNTVNIA
                     NPASYTNFILNLIRDDGAVVYVNGVEVVRSNMPAGIIGHATLAASTVGAPQESTPYQY
                     TIASSFFVNGANTIAVEIHQDAITSSDISFNMELLGSLDSTFNSSSADLNLPSCSQVL
                     FAGLYWGATQGTDGTNTSWITNETAVKLMIPGAANYTDITSTQTDYHNNTLVPGLPHT
                     GYRCFANITSLINATSPNGTYTLANLCSPAGIVNTAGGWTIVIAYSDPASIVRNLTVY
                     DGSVIMNGGDPALHVPITGFLTPPSGPVSCELGAVVFDGDRVSTDEFSFKQNSNPLVG
                     TYTNMTPNATANLNDMWNSTISYKGSVVTTRSPAHNNTLGYDADIIDVPNALNAVLGN
                     GQSSASIRFSSPSENYMLQVATTAISQYTPHFNVSKNSTDLNGGSLSPGDSILYRVDY
                     RNGGNDASTATTIIDNIPAGTSYAPNSLVISGVAKTDVAGDDEAEYDFVNNRVVFRLG
                     TGANSSTGGEINPGISGFVTFKVYTPSSCALFSCNNSISNRARMSYGGKLSLLSLEDS
                     SGVIIAGCNTPNPVSNIITGSCLPLGDTILTNTCPSLTVNIPVARYGGYKFFTGFPFS
                     GATAFNPANPITFTRIIYAFYDGPGSCDDTININVYITACPDIDDDDDGIPDYIEIDN
                     PVALQDHDSDGIPNWNDLTYPGYVDNNFDGFNDNFDPGADSDSDGNPNFYDTDFPGWF
                     DSNGDGVNDNMDKDLDGIPNNLDLDSDNDGIPDVVESFGVDANGDGRIDNYTDTDNDG
                     LSQNVDSPGGVAGSGMGLGALDTDGDGIPNYLDLDSDNDGIPDIIEAFGTDNNNSARV
                     TGFIDTDGDGYSDNVDGDVGNDNIAENSAFSLLRTGADGNNDGRCDTWPFINMDTDSK
                     PNPYDLDSDGDGLTDVKEAQFTDANWDGQVDGAINLNGRNTSLAALGSLIIPNSDGAA
                     RVDPFDIDSDDDGIPDNVEGLTTLGYLLPSAADIDGDGIDNSCDNFNGFGGDGIHPVD
                     TDGDIVPDYLDSDTDNDGLIDRIEGNDLNLNGQQDDLVTLLGIDTDGDGLDDRFDNNN
                     SSAEATSARMGNGGSTSGDPTPGSITTVQRTPVAFGCPTERDWRCLPYVLSCEFITFK
                     AILQNQMVQLDWTALCRQEVDYFIVERSTDRNNFGNAFITPGKPVLNEIEAYGGVDDI
                     NGITAAIIYYRLKSVMKSGRTILSNIIAVRRSTTEVVEIKILPNPVTDQLQLLINTHQ
                     NGIANIMIIDGTGRTIYHFKENLQKGSNTITYSQVAHLSEGIYYVRLQMEGAFITEKF
                     NKLK"
     gene            10483..11235
                     /gene="rsmA"
                     /locus_tag="PLO70_12580"
     CDS             10483..11235
                     /gene="rsmA"
                     /locus_tag="PLO70_12580"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQZ75347.1"
                     /translation="MYTLKKSLGQHFLKDENICRNIVSALQQYPFTHLLEVGPGGGAL
                     TKYLLELQKIEFKAVELDAEKVSYLLKTYPAIEGKIIHQSFLDIDKPFNGKFTVVGNF
                     PYNISTQILFKLLEWKEDVECMVGMFQKEVAQRIAAKEGNKTYGVLSVLVQAFFQVEY
                     LFDVHEKSFQPPPRVKSGVIRLLPVNEPLAISSEKQLFLLVKTAFNQRRKTLRNAVKG
                     LFDAAILADELFNKRAEQLSVAQFAALTFKMK"
     gene            11232..>12132
                     /locus_tag="PLO70_12585"
     CDS             11232..>12132
                     /locus_tag="PLO70_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ75348.1"
                     /translation="MSKVIITGKSHQYLIDQLAHKGYDVEYAPAITYDELAASIADAE
                     GIVVTTRIKADKKLLDKAKNLKWIGRLGSGMELIDVDYAESKGIKCVSSPEGNRNAVA
                     EHTLGLLLNLLNNISSSRDEVKQGQWKRDENRGVELSGKTVGIIGFGNTGSSFAKLLQ
                     PFNVTVLAYDKYKFDFGANYIKEASLEHIYRYADVISFHVPLTDETAGMANENFFNSL
                     QCKPYFLNSARGKVVNLDALIDALKQNKIAGAALDVLPNENLAAYTSEEKKQLEWLLQ
                     QPNVLITPHIAGYSHEASYKMAAI"
BASE COUNT         3589 a         2220 c         2693 g         3630 t
ORIGIN      
        1 tcttataact tggtagctgg ttattaccgc aggtaatacc tgcaaacctc ctctgcaaag
       61 aggaggtttt tttattgtag ctgtgctata ccagggcatt aatagcaaca caacatttat
      121 agatttgaga ggacagtttt accaaaggga taatatagtt gcatggaatt tttagttgcg
      181 ggcaattgat cttagatgtt tctatctgaa agcacctggc aaaaaaatat atttggaaag
      241 taagtttcag catataaagg atttttattg taaatacttt acaaaaaact acacgtaggc
      301 agaaactttc atctggataa aaaaggactt catttgtact ttgtataaat aattgatgtg
      361 tcagtcagag ttttcataaa tgcaataata tgggtaatct cattttttga aaggtttaat
      421 ttataaaaag gcagggtctg gttttcaggg gcgattttta atcctttgcc acctccattg
      481 ttataaaact ccattacttc ttccagggta tcatatactc cattatgcat gtaaggtgca
      541 gtcaaagcta tattccgaag tgtaggagtt ttaaaagcgt acttatgtac aattgaattt
      601 gtaaaattga atttgcccgg gtcgctatca agttcagcca tacttttatc ttttgatttt
      661 ggaacaccaa gtacttcgga ttccgtttct gcaaaaaaag gtggtacaac tccattaaat
      721 aatggaatga aatgacaggt tgcacatttc gcttttccgg caaaaagatt aaatcccttt
      781 ttttctgcaa gggataattg tttataatct ccccgcatat acttatcaaa tcttgaattt
      841 aatgattgca gtgaccggac atagcaggat atcgaattgg caatggtaaa agaatttatt
      901 ggatctcttt ccgattgata tgcagcctgg aagaatttag tatagtcatc agattttttt
      961 aactcgctga cactattttt taatgagccg ttcatttctt caacattatg tactacatca
     1021 cccaactgat tttcaagtat gtcagcccgg ctgtcaaaaa actgttttgt ctggaaaccg
     1081 gagttataaa gtgtgggggt gttgcgggcc agtaatgtta tcccatccag tgcaaaaggt
     1141 tttgcaaatc cgtctgtaaa agctttttcc gggaggtggc aggaagcaca acttcgggtc
     1201 ctggttccgg aaagtatagg gtcgctgaat aatagtttac cgagccggat cctttctggt
     1261 gttacccagt attcctcagg aggcgaatag aaattgatat tcagggcatc agcctcaaaa
     1321 atcgaaaggg aattaaaatt gatagaattt ctgccaccag ggatgccaat tacagattca
     1381 atccgggttt gtgccaacaa tttataaaat ggattaatat attgtgttat aaaagttagc
     1441 cggtcaaatt cattgaagtt attattacgt tgcaggtaac taactgattt atccagtaat
     1501 gcatttagtt ggacataaga attaacttta tttttttcga tcagcggttt aaaaaaagaa
     1561 agcagcgtct ttattcctgc taaacttgcc gttgcttcag gcaaagagta attagctgcc
     1621 ggagaatcaa aacctgtgat cccagttgta ataagctgaa cacaggcaga cctgaccgca
     1681 tcccagacca attcatcctt gaacttataa atcctgtcag gttcgttttc aagttttgaa
     1741 ataatctgta acatttggtc aagcaggtta atcacttcag gataggagct gtttgaccat
     1801 ttaccgctaa ataacagatc ctctatagcc tggaatccct gtggggggat gatcctgtct
     1861 ttaacttccg attcaatatg atcaatcggg ggaccattca atattttagt ttcatattgg
     1921 ttaaagtaat ctgttaatac cgataacttt ttataggtca gcctgctttg gtaaaacctg
     1981 gctttcaggt ctgtagtagt ttcttttttt tgacaggaag ttttaaagga agtaatgctt
     2041 tttttaaaga catccagctg ctgagtatag tatttattta tgttcatgcc cattgtagct
     2101 gattcaggat gtaagtgagc catgagaata aacatgctga atactgcaaa aatgaccgta
     2161 attattcgtg tggattttac ctgaaacata tttatgtctt aattagtttt tctttaaagt
     2221 cctgttggtg acgaatttat gacgatttaa ggaattgttc ttagctgcta tgtaaataaa
     2281 cgattagtat attttcaaat gacttgcaat tcatttttct atttatcata tttacattta
     2341 ataaccaata acctaatctc atgaaaccac tttcttccac cctcaaggct ctaatattat
     2401 atctaataat tacttcagtt gcatttaatt ccttttcaca gacaactatt gttgcctatg
     2461 gaagcagttg gaaatatctt gacaataata cccgaccggc aaattgggaa acttcagcct
     2521 tcaatgatgc agcctgggca tctggaaatg gacaattggg ttatggcgat ggagatgaag
     2581 ctaccgttgt aaatcatgga gggtgcagtc caattgctac ttgcggccct aagtacatta
     2641 ctacttactt caggaaatcg atcaatatta ccgatccatc ggcatatgta aattttacac
     2701 ttaatgtgtt aagggatgac ggtattgtta tttatatcaa tggaacagag cggtatagta
     2761 ataatatgcc agctggccga ttgcacagta ctttagctac tgctgcggca tccgatgatg
     2821 gggccactcc ccaaacagca acactttctt cttcgttttt ttcggctggg acaaatgtta
     2881 tagcagtaga gatacatcag aacagtacta caagttcaga tataactttc gatttagagc
     2941 ttttgggtaa tgcagcacca tccgggttga ttaattatgg cagtacctgg aagtaccttg
     3001 ataataatac acggcctgct ggttgggaga caattgcatt taatgatgtg gcctggagca
     3061 gcggtctatc cgaatttggt tatggtgacg gtggtgaagc tacctgcgta gcttcagggg
     3121 gtggagggac attatgcaca ccaaccggga acaagtatat taccacttat ttccggaaaa
     3181 catttaacgt aactggcctg aatacattta acagttttac attaaatatt atcagggatg
     3241 acggtgttgt ggtatatatt aacggaaccg aagtagcaag gaataatatg ccgggaggca
     3301 cacctgccca ttctacatta gcgtcagcag caattggagg caccgatgaa actacaccaa
     3361 taacatttag tctttcccct tgtgcttttg tggagggtac taacactatt gctgtagaaa
     3421 tgcaccaaaa tgatgttacc agctctgatc tttcttttaa tttggagtta aacggtattg
     3481 ttggtggtgg tacaccaaca ttaacccgcg ggccatattt gcaagtggca agtgagacag
     3541 ccattacttt tagatggaga acaagtacgg cgtgttatgg tcgtgtaagt gtggggcctt
     3601 caaatggcac ttacaccact gcaacagtgg atgaaacctg ccctacgaca gagcatatca
     3661 tcagggtaac cggacttgct tcggatacaa aatattttta ccagatttca gcaactgatg
     3721 gcactgtttt ccagggcgct gctgataatt ttttcagaac aaaccctccg gcaaatacca
     3781 caagaaaaat tagaattgct gcttttggtg attgtggcag gaataatgca acgtaccagg
     3841 ataataatct ggctaactac cagaattatt tatctgttaa tggaattgat gcccctgatg
     3901 cgtggatcct gatgggtgat aatgcctata attccggcac cgatgcagaa tacacaacta
     3961 atttctttaa tatttacggg aataatattc ttaagaatca taaattatat ccttcgccgg
     4021 gaaatcatga ttatggtaac agttcgacta ataaaccgtc ccgctcaatg ccatatcata
     4081 ctaatttcag tgttccacag aatggcgaag ccggtggtgt tgcttccaat aaacaaaatt
     4141 tttattcttt tgatgttggt aatatccatt ttctttcttt agactcttat ggtacagaat
     4201 cggatctaac cgacatgcaa actgccggag ccagtgcact aaaaacatgg ctggatgcag
     4261 atcttgctgc taatacaaag aagtgggtga ttgcttactg gcaccacccg ccatatacga
     4321 agtcgagtca taattcagat agtgagagtg accttgtaaa tatccggcag aactttatca
     4381 ccttcctgga aacccgtgga gtagatatga tcatttgtgg tcatagtcat gcttacgaga
     4441 gaggttacct gttgcggaat tttaccggtg cctggacttc ctttaatcct gcaacacatg
     4501 cagtaagtac ttccagtgcc acgtatacga gtaactcaac ctgtccttat gtgtataata
     4561 caacacccgc taatcatggt actgtttatg tagtagcagg gtctgccggt gccagcggag
     4621 gcaccaatgc cggctttgga gccaatgcaa tgccatttgc agtgaatgat gccggactgt
     4681 tttattttga agtggaagat aacaggctgg atgcaaaaat gctccgccgt aacggaacaa
     4741 tctttgatca gttcactatc atgaaagatg taaaccaggt aaccaattat aatattccaa
     4801 atgggtcatc acaaacactt actgcatcat ggccgggaaa ttatagctgg agcacatcag
     4861 ctacaacgag ggctgtaaat gttacacctc ccaataacgc agttacaaac tatacggttt
     4921 ccgatgattt cggttgtatc accgatcaat tctcagttac cacatccggg acattgcccg
     4981 tttcgctttt gacctatgat gtaaggttaa ctggtaataa agtaaatact acatggtcta
     5041 cttcgactga ggtcaataat agctttttct ctattgagag atcttctaat ggaagagatt
     5101 ttatagttat agggaatgta aacggggctg gtaacagtac cactaacagg acttatgctt
     5161 ttacagatgc acagccactt ttgggtacca gttattaccg tttatctcaa accaattttg
     5221 atggtcaaag agaatacatg ggtgtgagaa gaatagaaaa cacttccctg aaagaatttg
     5281 aggtaaagac tatttcaggt tatgccggta aattaatact tcagatcaat tcatcagttg
     5341 atggtgttta tcagctcagc attgtcgata tgacgggcag aaaaataaaa aatgaagtgt
     5401 tgggaataaa cgccggtatt tcgacaaaag agatttacct gaacccggga gtttatatct
     5461 gggaagtacg taatagcaaa ggtgatgcta tgtttcaaaa agtgatagtt cagtaagacg
     5521 tttattaaac cttatatccg tactatgaaa aaaatttatg tcatagttgt gttgttactt
     5581 ctgtgcctcg tgcaacaggg gtacagccag gtgttacgat cgatgacacc ccggtataac
     5641 aatgcctctg ttaaaggcaa tattgttttc gtttctaata atattattac atcatccggt
     5701 gctattacaa cagaagcgcc tccgggcggt accgctacaa acaatggcca cacgggtttg
     5761 aatgttgatg tggatggaac ccctccgacc tcttatatcg cttttggtag taactggaag
     5821 tacctggata ataataccag gccggttgga tgggaaacag tagcttatgt ggatgctgcc
     5881 tgggcagcgg gtaatggtca actgggatat ggagatggtg acgagactac tattgtaaac
     5941 catggcgggt gtgctcctat tgccacttgc ggaccaaaat acatcaccac ttacttcaga
     6001 aagacaatta atattcctaa ccccgcaacg cacagcaatt ttacattgaa tgtaagaagg
     6061 gatgatgggg tggcggtata tgtgaacgga gtggaggtct acaggaataa tatgcctgcc
     6121 ggggtgcttg cacatggaac tttagcaagt gccgccgctg cagacgatgg caatacagta
     6181 caaactgcta caatagcttc cggtgcattt attgccggta ataatgttat tgctgtagag
     6241 gtacaccaaa ataacaatac cagttcggat ctcagttttg atcttgaatt actcggaaat
     6301 ccaataacaa cagtaaccgt ggttccctat ggagcaaatt ggaaatacct ggctaataat
     6361 acaagaccag ctggctggga aactgttcct tatgttgatg cggcctggcc agctggtaac
     6421 gcagaatttg gatatggtga tggagatgaa gccacttgtg ttccttcggg aggaggtggt
     6481 acactatgtg cgcctacagg gaataaatgg ataactacct atttcaggaa tacggtaaat
     6541 attgccaatc ccgcttctta taccaatttt attctcaatc ttatcaggga tgacggagca
     6601 gttgtttatg taaacggggt ggaggttgta aggagtaata tgccggctgg gataataggg
     6661 catgctacat tggctgcttc aacggtaggt gccccgcaag agtctactcc ttatcaatat
     6721 actattgctt caagcttttt tgtaaatggg gccaatacta ttgcagttga aatacaccag
     6781 gatgcaataa cgagttcaga tatttcattc aatatggaac tgctgggttc tctggattcg
     6841 acttttaact catcaagtgc cgatctgaat ttacccagtt gctcccaggt tttatttgcc
     6901 ggtctctatt ggggtgctac ccagggtaca gatggaacta ataccagttg gataacaaac
     6961 gaaacagctg tgaaactgat gataccgggt gctgccaatt atactgatat tacttctacg
     7021 cagacagatt atcataataa cacattagta cccggtttac cacatacggg atatcgctgt
     7081 tttgcgaaca taacctcatt gataaatgct actagcccca atggtactta tactttggct
     7141 aatctttgca gtcctgccgg gattgtaaat accgccggtg gatggacaat tgtcattgct
     7201 tattcagatc ctgcttctat agtccgtaat ctgactgtat atgatggttc agttattatg
     7261 aatgggggtg atccggctct tcatgtgccc attacaggat ttttaacacc tccttcgggg
     7321 cctgttagtt gcgagttggg tgctgtagtt ttcgatggag acagagtttc taccgatgaa
     7381 ttttcattta aacagaattc aaatcccctg gtaggcactt acacaaatat gactcctaat
     7441 gccacagcta atttaaatga tatgtggaac agtaccatct catataaggg atcggtggtt
     7501 actactagaa gcccggcaca taataatacg ctgggatatg atgctgatat tattgatgtt
     7561 cccaatgcat taaatgcagt tttggggaat ggtcaatcct ccgccagtat tcgttttagt
     7621 tcgccttctg aaaattatat gttacaggtg gctaccaccg ctatttctca atacactcca
     7681 cattttaatg taagtaaaaa ttctacagac cttaacggtg gttcattgag ccccggagac
     7741 tccattcttt atagagttga ttacaggaac ggaggaaatg atgccagtac tgccacgact
     7801 attattgaca atattcctgc cggaacttct tatgctccca atagtcttgt tataagcgga
     7861 gttgctaaaa cggatgtggc aggtgatgat gaagctgagt atgattttgt taataaccgg
     7921 gttgtattca gactgggtac tggcgcaaac agttcaaccg gtggcgaaat aaaccctgga
     7981 ataagcgggt ttgtcacttt taaagtatac actccgtcat cttgtgcatt gttttcctgt
     8041 aataactcta tcagtaacag ggcaagaatg agttatggag gaaaactttc tttattaagc
     8101 cttgaagatt ccagtggagt tataattgca ggatgtaata ctcccaatcc tgtgtccaat
     8161 attattacag gatcttgttt gcctttgggc gatactattc ttactaatac ctgcccctct
     8221 ttgacagtaa atatccctgt cgcccggtat ggtgggtaca agttttttac cggatttcct
     8281 ttttcgggtg ctaccgcttt taatcctgca aatccaataa cttttaccag gattatttat
     8341 gctttttatg atggtccggg ttcttgtgat gatacaatca atattaatgt atatataaca
     8401 gcttgtcctg atattgatga tgatgatgac ggtataccgg attacattga aattgacaat
     8461 cctgtagcac tgcaagatca tgatagtgat ggtatcccaa actggaatga tttgacatat
     8521 ccggggtatg tagataataa ttttgatggg ttcaatgata attttgaccc gggtgctgat
     8581 tccgattctg atggaaatcc aaatttttat gatactgact ttcctggttg gtttgatagt
     8641 aatggcgatg gggtgaatga taatatggat aaagatcttg atggaatacc caataacctg
     8701 gatctggata gtgataatga tggaataccc gatgtagtag aaagttttgg agtggatgca
     8761 aatggtgacg gacgaattga taactatacc gatacagata atgatggatt atcgcaaaac
     8821 gtagatagtc ctggtggcgt agcaggctca ggcatgggac ttggggcttt ggatacagat
     8881 ggggatggta tccctaacta tttagacctg gatagcgata atgatggtat accggatatt
     8941 attgaagcgt ttggtactga taataataac tccgccaggg tgactgggtt catcgataca
     9001 gatggggatg gctattccga taatgtggat ggagatgttg gtaatgataa tattgctgag
     9061 aattcagcat tctctctctt aagaaccggt gctgatggaa acaatgacgg aagatgcgat
     9121 acctggccat ttataaatat ggataccgat agcaaaccga acccctatga tctggacagt
     9181 gatggtgatg gtttaactga tgtgaaagaa gcacagttta ccgatgcaaa ctgggatggg
     9241 caggtcgatg gtgctatcaa tttaaatggc cgcaatactt cacttgcagc tttgggttca
     9301 ttaattattc ctaattctga tggggctgca agggtcgacc catttgatat tgattcagat
     9361 gatgatggta tcccggataa tgtggaagga ttaactactc ttggctactt gttaccctcc
     9421 gctgcagata ttgatgggga tggtattgat aactcctgcg ataattttaa tgggtttgga
     9481 ggtgatggaa ttcaccctgt agatacagat ggtgacatag tgcccgacta tcttgattca
     9541 gatactgata atgacgggct aattgacagg atagagggga atgatttaaa tcttaatgga
     9601 caacaggatg atctggtgac attattgggt attgataccg atggcgatgg gttggatgac
     9661 cggtttgata acaataattc cagtgctgaa gccacatcag cccgtatggg taatggtggc
     9721 agtacatccg gagaccccac accaggttca ataactactg ttcagcgaac acctgttgcg
     9781 tttggttgtc cgaccgaaag agactggcgt tgcttaccat atgtgttgag ttgcgagttt
     9841 attactttca aggccatact gcaaaaccag atggttcaat tggattggac tgccctgtgc
     9901 agacaggaag tagattattt tatagttgaa agaagtacgg atcggaataa ttttggtaat
     9961 gcatttatta ctcccggtaa acctgtacta aatgaaattg aagcctatgg tggagtggat
    10021 gatataaatg gtataacagc agcaataata tattaccgat tgaaatctgt aatgaaatca
    10081 ggccgtacga tcctgagtaa tatcatcgct gttcgacgca gcactaccga agtggtggaa
    10141 ataaaaattc tgcccaaccc tgtaactgat caattgcagc tgctgatcaa tacacatcag
    10201 aatggaatag ctaatattat gataattgat ggcaccggac gtactattta ccattttaaa
    10261 gagaatttgc aaaagggaag taatacgatt acttattcac aggttgcgca tctttcagag
    10321 ggcatctatt acgtcaggct tcagatggaa ggggctttca tcacagagaa attcaataaa
    10381 cttaaatagg tagctggtta ttaccgcagg taatacctgc agacctcccc tgcaaagagg
    10441 aggtttttta ttgtagctgt gctatcagtt acatttgcac atatgtacac cctgaagaaa
    10501 tcattgggcc agcattttct gaaagatgag aacatttgcc ggaatattgt ttcagcttta
    10561 caacaatacc cttttactca tttgctcgag gtgggtcccg gtggcggtgc gttaacaaaa
    10621 tacctgctgg agttgcagaa aattgagttt aaagctgtag aactggatgc agaaaaagtg
    10681 tcttatttac tgaaaaccta cccggcaatt gaaggaaaga tcattcacca gagtttcctg
    10741 gatatcgata aaccatttaa cgggaaattt acagtagttg gcaattttcc ttacaacatt
    10801 tcaacacaaa tactgtttaa actactggaa tggaaagagg atgtggagtg catggtgggt
    10861 atgtttcaaa aagaagtagc ccagcggata gcagcaaaag aaggtaataa aacctatggg
    10921 gtattaagtg tgctggtaca ggcttttttt caggtggaat acttatttga cgtccatgaa
    10981 aaaagttttc aacccccgcc cagggtgaaa agtggagtaa tacgtttact gcctgtaaat
    11041 gagcccctgg ctataagcag tgagaagcaa ttatttctat tggtaaaaac cgctttcaac
    11101 cagcgcagga aaacccttag aaatgcagta aaagggttgt ttgatgcagc gatattggcc
    11161 gatgagttgt ttaataaaag agcagagcag ttgagcgttg cgcaatttgc cgcacttact
    11221 tttaaaatga aatgagcaaa gtaataataa cggggaaatc ccatcagtat ttgatcgatc
    11281 agttagcaca taaaggatat gatgtggagt atgcccctgc tattacttat gatgagttgg
    11341 ctgcttccat tgctgatgct gaaggaatag ttgtgacaac gaggatcaaa gcagataaga
    11401 agctacttga taaagcaaag aatcttaaat ggatcggaag gcttggcagt ggaatggaat
    11461 tgattgatgt tgattatgca gaaagtaaag ggataaaatg tgtaagcagc ccggaaggta
    11521 accgaaatgc tgtagcagaa catacgttgg gactattgtt gaacctgctt aataatatta
    11581 gttcttcccg ggatgaagta aagcagggcc agtggaaaag agatgagaac aggggtgtag
    11641 agctaagtgg taaaacggtt ggtatcatag gatttggaaa tacaggcagc agctttgcca
    11701 aattgttaca gccttttaat gttacggtgc ttgcatatga taaatataag tttgactttg
    11761 gcgctaatta tataaaggaa gccagtcttg agcacattta tcgctatgca gatgttatca
    11821 gttttcatgt acccctgacg gatgaaactg ccgggatggc aaatgaaaac ttttttaatt
    11881 cactgcagtg taagccttat tttttgaaca gtgcaagagg aaaagtcgtg aacctggatg
    11941 cattgataga cgctttaaaa caaaataaga tcgccggtgc agcattggat gtattaccta
    12001 atgaaaacct ggcggcctat acttcagagg agaaaaaaca actggaatgg ttattacaac
    12061 agccaaatgt cctgattacg cctcatatag ctggctatag tcatgaagct tcttataaaa
    12121 tggcagcaat tt
//
LOCUS       DAORTI010000109        12055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5373_length_12055_cov_6.597417, whole genome shotgun sequence.
ACCESSION   DAORTI010000109 DAORTI010000000
VERSION     DAORTI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_12055_cov_6.597417"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..3275
                     /gene="secDF"
                     /locus_tag="PLO70_12590"
     CDS             159..3275
                     /gene="secDF"
                     /locus_tag="PLO70_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HQZ75349.1"
                     /translation="MQLKGLVRFFTVLLIVYSLYQLSFTWLVKNHEKKMEAKARNYVK
                     NNFQSAAAKYPANKDSQAIYQEELDKIFQKNRDSLIKATENTTVTYGINGAMSYKKAK
                     EEELNLGLDLQGGMNVTLEVEMTGLLKSLSNNSKDPNFLSALVNADKRKANSDADFIT
                     LFIQEYRKLEATRPLATLFSGASSNAIDINSSDNKVESYIREQARSAFNLTFDRLSTR
                     IDQFSVAQPSINPIPNRGLINVELPGIKDKERVRKLLQSTANLQFWEVYTLQDNNYAN
                     GWQRTILAFNAKYGGVEDTTAKKDTTPAVVKDEDTTKTKKLSDDITKTADDTADKKKT
                     ADLATGKITLDKYLQFGQPYRDEKGNVSYPSAIGAVAINDTALFRTYLNEFKSNFPSD
                     VVWAYGVPEKDKKGKTASSVPLYALKTYNRTEAKLEGDAISDARQDFDQYGKVQIEMT
                     MKPAGASIWKKMTEENVGKPIAIVLDNVVHSAPNVINPIPNGISSISGSYTQQEAADL
                     ANILKIGKLPAPAKIVQEQQVGPTLGEEAIRGGSLAFIISFVVIFLLMLIYYNTSGWV
                     ANIALILNLLFTVGILAGLGATLTAPGIAGLVLTIGMAVDTNVIIYERIKEELTKGKG
                     YIPAINEGYKRSLPPVLDGHITTLLTAFILFYFGLGPVKGFATTQIIGILLSLFCGIL
                     VSRWVSEWFTNKKKHLEYFTGVSRRVFKHAKYKFIEYRKVAYIISFVVLALGIASFFN
                     GFNYGVEFNGGRSYTVQFDRKVDEEKVRQDLNKAFEGENTIIKTIGDNSKLDITTSYL
                     IKDSRSISDSLVEVKLFEGLKSYLPANISFEQFKTDKYLPQSKKVLPTISDDLKAGAV
                     KATLFALLVIFLYIFIRFRDWRYSLGTIVALLHDVLVTLIVFSFAKNIVPFPLEIDQH
                     FIAALLTVIGFSMNDTVIVFDRIREDSRLMPNAPRGEIINRAINETLSRTIMTSLTVF
                     LTILILFLVGGEVTRGFAFAMLIGVVTGIYSSVFVAAPILVDLGGKRQLGKKAVIEKT
                     KPGSTPKS"
     gene            3339..3587
                     /locus_tag="PLO70_12595"
     CDS             3339..3587
                     /locus_tag="PLO70_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75350.1"
                     /translation="MLQQLTILNTGITIFCVYGFLGTLLSSGGPWGFGYLVVWIFPVI
                     WIIGIFIDLLLKSMIKDRILLNIAGVIIIMLFLLIISP"
     gene            complement(3663..4007)
                     /locus_tag="PLO70_12600"
     CDS             complement(3663..4007)
                     /locus_tag="PLO70_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQZ75351.1"
                     /translation="METTTISPVTLTEGAIVEIKKLMAADGFDTTQKLRIGVKGGGCS
                     GMTYVLGFDQVKENDTHFEINGIACIMDKAHEIYLFGMQVDWQDGLNNRGFTFKNPNA
                     STTCGCGTSFAV"
     gene            4157..4660
                     /locus_tag="PLO70_12605"
     CDS             4157..4660
                     /locus_tag="PLO70_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75352.1"
                     /translation="MDLTTLSLIISVLAIVIAVYSFTQKPKKSADPVVKDKFESIPLR
                     LQAYERLVLLTERIALPNLISRLNQPGISAAEMKIILTENIKQEFEYNSTQQLYVSPV
                     SWDAVRNLKEQNIMVINQVAGTLPPAASASELNKKILEILLSQQSGAIHEMVAQTLNH
                     EAKKLMQ"
     gene            4688..6247
                     /locus_tag="PLO70_12610"
     CDS             4688..6247
                     /locus_tag="PLO70_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQZ75353.1"
                     /translation="MDPKKKYTDSGIEIKSVYTHDDLPATKSENPGEFPFTRGVQPDM
                     YRGKLWTMRQYAGFSTAEESNKRYHYLLSQGVSGLSVAFDLPTQIGYDSDHPLAEGEV
                     GKVGVAIDSIEDIQTLFNGIKLEDVSTSMTINATGFILLSFYVALAKQQGADLKKISG
                     TIQNDILKEYAARGTYIYPPKPSMRIITDIFEWCSNEVPKWNTISISGYHIREAGSTA
                     VQEIAFTLSNGKAYVEAALKKGLDINVFGKRLSFFFNAHNNLFEEVAKFRAARKMWAK
                     IMKDLGATDPKAMMLRFHTQTGGSTLTAQQPLNNISRVTIQTLAAVLGGTQSLHTNGY
                     DEALSLPTEEAAKIALRTQQIVAFESGAPDSVDPLAGSFFIESLTREVEEAAWRLIEK
                     IDSLGGSVPAIEQGFIQNEIARSAYEYQKNIETGEKIIVGVNKFQTEDHHKIPILKVD
                     DSIRTLQIEKLEKLRNNRNHAKCDQLLQILNDKVTSGENIMPAVVEAVENKCTLGEIA
                     DTLREVYGEYK"
     gene            6350..7891
                     /locus_tag="PLO70_12615"
     CDS             6350..7891
                     /locus_tag="PLO70_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQZ75354.1"
                     /translation="MKHTRLITICLLLLISSLSNAQQPSFVSDSLDSYIERGMKQWQI
                     PGLAIAIVKNGKVVLSKGYGVRELGKDDKVDENTLFIIASNSKLFTGTAIAKLDHEKK
                     LSLNDKVTQYIPWFRLYDSTSGKLANIRDMLCHRLGTKTFQGDFTFWDSNLPKDSIIW
                     KMRYLKPTGEFRQDYGYCNSAFLVAGEILTKVTGMSWENYVQENMLTPLGMRNTYMNT
                     TGLANRVNVAAPHNNLFSSITKIPFDNVDNLGPATSMVSNVNDLTKWLLLQLDSGKYM
                     GKQVLPWAVLQKTRDINIITGSRKSTVYPTHFRGYGLGVYSTDYAGKQVYWHTGGAFG
                     HVTNVCFVPEENLGITILTNNDNQSFFEALRYQLLDAYLGVSYADRSKFQWGFFEQGK
                     KKLDEELSALQEKVNKKNLPEIKPENFTGDYFNTVYGKITITKSGNMLICRFQHHPDL
                     IGYMEYMDNNEYRLTYSNIGYGIYPAKFSIKDGKVIAVEIKANDFVESDAYIFVKDSL
                     DGVSK"
     gene            complement(7967..8347)
                     /locus_tag="PLO70_12620"
     CDS             complement(7967..8347)
                     /locus_tag="PLO70_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75355.1"
                     /translation="MSYTNITRIGTDHTIWQKGFGFYKDELDIMESRLSEVALKNTTF
                     EARQGVEHFQNQFIVQRNNIDELKHEVNLYVEKLGKDAQQHGGRMETAMIAEHTALHE
                     KYEDFERVMNILRHEFNEYLSKWM"
     gene            complement(8566..10227)
                     /locus_tag="PLO70_12625"
     CDS             complement(8566..10227)
                     /locus_tag="PLO70_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQZ75356.1"
                     /translation="MSEERSLNFLEEIIEEDLKNGKYKSIVTRFPPEPNGYLHIGHAS
                     SICLNFGLTQKYPGYTNLRFDDTNPVTEETEYVESIKEDVKWLGFEWKHERFASDYFD
                     TLYEYALKLIDKGLAYVDDSTAEEMAVMKGSPTEPGKDSIYRDRTIAENRDLFVRMKN
                     GEYPDGSRTLRAKIDMAHINMLMRDPILYRIKHAHHHRTGDKWCIYPMYDFAHGQSDS
                     IEEITHSICTLEFVPHRELYDWLIEKLEIYPSRQYEFARRNINYTVTSKRKLLQLVNE
                     NHVTGWDDPRMPTISGLRRRGYTPDSIREFCDRIGVAKRENMVDVGLLEFCVREHLNR
                     IALRRMVVFDPLKVVITNYTGEEEILHSENNPEDEKGGTREIPFSAELYIEKEDFMEN
                     PPKKYFRLAPGQMVRLKSAYIIKCDEVIKDAAGTIAEVRCTYIPESKSGSDTSGINVK
                     GTLHWVSVKHAINAEVRLYDRLFKVEDPTDEEGDFKEYLNPGSLEILSTAYAEPVLKN
                     ATLEERYQFIRKGYFVLDKESAPGKLVFNKTVEKKDAWAKEVKKS"
     gene            10481..11326
                     /locus_tag="PLO70_12630"
     CDS             10481..11326
                     /locus_tag="PLO70_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ75357.1"
                     /translation="MKKILVPTDFSLNADRAIDYAVQIAKLNEATIYLIHACDHLDTL
                     YMESAIPEREYNKAITDAAFDKLEILRRSIEETEQVLVNIQLYSGPINDTILVAAEEH
                     NVDLIIMGTLGISGIKDKIFGSRTASVISKSIVPVLAIPLEYDWSPPAKLLLAINRFD
                     EVSDLLNPVFDMATIFNAEVNITVFTDEDNAAASDYLEDAKEVKLAEEKLRRKFSKLV
                     IKAEHLSGRYFEDSINEHISKNKIDLLAMTTHKRSFIGSIFNRSITRKMSYHSKVPIL
                     AIPVK"
     gene            11356..>12055
                     /locus_tag="PLO70_12635"
     CDS             11356..>12055
                     /locus_tag="PLO70_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ75358.1"
                     /translation="MKTILIPFDFSANARKALAYALELGKLFESQLYIYHVLHESPYT
                     LAAAATEQEMEKLIAKDGEEKTAALKDEVNTLLQSLPITFSAERIKTKAEYNPLVVEK
                     IIAVAKEQDAELVIMGTHGTSGIKKFFFGSNTSNMITKSDIPVLAIPEQYEYTPVKTI
                     LYASDLENLPAELKRIIPFAHSLHAGINVLHFDYGQNERKITEEEANIIIKNNQYQHI
                     KFVKEKADLTIPLLK"
BASE COUNT         3751 a         2503 c         2340 g         3461 t
ORIGIN      
        1 aagcttttaa aaatcagccc tcaagaaccc gtttttagtt ggccggaatt aatctgaaac
       61 agctatcttt gccgtcctta aaatttctgt aaactcttta ttatccagtc aacataaaca
      121 ctgttgataa agagataaaa ttgtaaatcg atcaacgtat gcaactgaaa ggtttggttc
      181 gctttttcac agttctgctt attgtctatt ctctctacca attgtctttt acctggttag
      241 ttaaaaacca tgaaaagaaa atggaagcaa aagccaggaa ttatgtaaaa aataattttc
      301 aaagtgctgc agcaaaatat cctgccaata aagattcaca ggctatttac caggaagaat
      361 tagataaaat attccagaag aatcgtgata gcctgattaa ggcaacagaa aatacaactg
      421 taacctatgg catcaatggt gccatgagtt ataagaaggc caaagaagag gaattaaatc
      481 ttggtttgga tctccaggga ggaatgaacg taacactgga agtggagatg accggtttat
      541 tgaaatcgct gtccaataat tccaaagacc ctaatttctt atctgctttg gttaatgcag
      601 ataaaagaaa ggcgaacagc gatgctgatt tcatcaccct tttcattcag gaatacagaa
      661 agctggaagc aacaagaccg ctggccactt tgttctccgg tgccagttca aacgcaattg
      721 atattaattc aagcgataac aaagttgaaa gttatatccg tgaacaggcc agatctgcat
      781 tcaaccttac cttcgacagg ttaagtaccc gtatcgacca gtttagtgtt gcacaacctt
      841 ctatcaaccc tattccgaac aggggattga tcaatgtgga attacccggt atcaaagaca
      901 aagaaagggt aagaaaactt ttacagagta cggcaaatct tcaattctgg gaagtttaca
      961 ctttacagga taacaactat gctaatggat ggcaaagaac aatccttgct ttcaatgcta
     1021 aatatggcgg agttgaagat actaccgcta aaaaagatac tacaccggca gttgttaagg
     1081 atgaggatac caccaagact aaaaaacttt ctgacgatat caccaaaaca gctgatgata
     1141 ctgctgacaa aaagaaaact gctgaccttg ctaccgggaa aataacactt gataagtacc
     1201 tccagtttgg ccagccatac cgtgatgaaa aaggtaatgt ttcttatcca tcagccattg
     1261 gtgctgttgc tataaatgat acagccctct tcaggactta cctcaatgag ttcaaatcca
     1321 atttcccttc cgatgttgtt tgggcatacg gggtacctga aaaggataaa aaaggtaaaa
     1381 ctgcttcatc agtaccactg tatgcattga aaacatacaa tcgtaccgaa gcaaaactgg
     1441 aaggagatgc tatttcggat gcccgccagg actttgacca gtacggcaaa gtgcagattg
     1501 aaatgacaat gaaacctgcc ggggcaagta tctggaaaaa aatgactgaa gagaatgtag
     1561 gcaaacccat tgcgatcgta ctggacaatg ttgtacattc tgctcccaat gttatcaatc
     1621 ctattccgaa cggaatttca tccatatccg gcagctatac ccaacaggaa gcagctgacc
     1681 ttgctaatat attgaagatt ggtaaattgc ctgcccctgc caagatcgta caggaacaac
     1741 aggtaggtcc tacgttgggt gaagaagcta tccgtggtgg ttcattggca tttatcatat
     1801 cctttgtggt tatctttttg ctgatgctaa tatattacaa caccagtgga tgggttgcaa
     1861 atattgccct tattttgaac ctgctcttta cagtaggtat tctggccggt cttggtgcta
     1921 ccttaacagc gcctggtatt gccggcctgg tattaactat tggtatggca gtagatacca
     1981 acgtaatcat ctatgaacgt atcaaagaag aactgacaaa aggcaaagga tatatccctg
     2041 ctatcaatga aggatataaa cgttcattgc cacctgtgct ggatggtcat ataaccaccc
     2101 tcttaacagc cttcatatta ttttatttcg ggcttggccc ggtaaagggt tttgccacta
     2161 cccagatcat tggtattctt ttatccctgt tctgtggtat cctggtgagc cgttgggtgt
     2221 ctgaatggtt taccaataag aagaaacacc ttgaatattt taccggtgtt tcccgccgtg
     2281 tgttcaaaca cgccaaatac aagttcatcg aataccgcaa agtggcctat atcatttcat
     2341 ttgttgtatt ggcgttaggt attgcctctt tctttaatgg atttaattat ggtgttgaat
     2401 tcaatggagg tcgcagctat actgttcaat ttgacaggaa agtagatgaa gaaaaagtaa
     2461 gacaagacct gaacaaagca tttgaaggag agaacaccat cattaaaacg atcggtgata
     2521 attctaaatt agatatcact acatcctatc ttattaagga tagccgttct atatctgatt
     2581 cgctggtaga ggtaaaatta tttgaaggtt taaagagcta cctgcctgct aatatcagtt
     2641 ttgagcaatt taaaacagac aaatacctgc cgcaaagtaa aaaagtatta ccaaccatat
     2701 cagatgactt aaaagccggg gctgtaaaag ctactctttt tgcattgctg gtgatcttcc
     2761 tgtatatctt catccgattc cgcgactgga ggtactcact tggtacgatt gtggctttgt
     2821 tgcatgacgt attggttaca ctgattgtat tctcttttgc caagaatata gtgcctttcc
     2881 cacttgagat tgaccagcac tttattgctg cgctattaac ggtgataggt ttctctatga
     2941 atgacaccgt aatcgtattt gaccgtatcc gtgaggacag ccggttaatg cccaatgcac
     3001 cgagaggtga gatcatcaac agggcgatca atgaaacatt gagtcgtacg attatgacat
     3061 cattaacggt attcctgacc atcctgatcc ttttcctcgt gggtggtgaa gtaacaagag
     3121 gttttgcatt tgccatgctt atcggtgtgg taaccggtat ttattcatcc gtattcgtgg
     3181 cagccccgat attggttgac ctgggtggta aaaggcaact gggcaaaaaa gcagtaatag
     3241 aaaaaacaaa gcctggctct actcccaaga gttaagcttc ggcatttgtt tatgaacttt
     3301 atagttaaat cctgtggtaa attgccacag gattttttat gttgcaacaa ctcacaatac
     3361 taaataccgg cattaccatt ttttgcgttt atggtttcct gggaactctt ttatcttcgg
     3421 gaggaccatg gggctttggc tacctggtag tctggatttt tccagttatc tggatcattg
     3481 ggatattcat tgatctgttg ttaaagagta tgatcaaaga caggatttta ttgaatattg
     3541 ccggtgttat tataattatg ctctttttgt tgatcatcag cccataaaaa aagcttcctg
     3601 acagaagctg caattattca ttaaaaaaaa gagaacaagt aagttgttca ataaagcggt
     3661 ctttatactg caaaggaagt cccgcatccg caagtggtgc tggcattggg atttttaaaa
     3721 gtaaaacccc ggttatttaa tccatcctgc caatccacct gcatcccgaa taagtatatt
     3781 tcatgggctt tatccatgat acaggcgatt ccatttattt cgaagtgagt atcattttcc
     3841 tttacctggt caaaacccaa cacataggtc ataccggaac atccgccacc ttttacgcca
     3901 atccttaatt tttgcgttgt atcaaaacca tccgctgcca tcagcttttt aatttcaacg
     3961 atggctcctt ccgtcagtgt aaccgggctt attgttgtcg tttccatgtt gtaaaattaa
     4021 atattctttt tacagttttc ttattatcgc ttttcgaaga aaggaattta actttcccta
     4081 tccctgctgt ggaacttttg accctgatct ttggataatc agcttatttt tgctgttcaa
     4141 accaaaccac catccgatgg atttaaccac attgagcctt attatctccg tattagctat
     4201 cgtaatagct gtttactcct ttacccaaaa acctaaaaaa tcagcagacc cggttgtaaa
     4261 agataaattt gaaagcatcc ccctgagact acaggcgtat gaacgattag tgctgctgac
     4321 tgaaagaata gctctcccca atcttatcag ccgcttaaat caacctggca tttcggctgc
     4381 tgaaatgaaa atcatcttaa cagaaaatat aaagcaggag tttgagtaca acagtacgca
     4441 acaactttat gtaagccctg tcagttggga tgctgtcaga aacctgaaag aacaaaatat
     4501 catggtcatt aaccaggttg ccggcacgct gccaccggct gcatctgcat cggaattgaa
     4561 taagaaaata ctggaaatat tattatcgca gcaaagcgga gccatacatg aaatggtggc
     4621 acagacgctg aaccatgaag caaaaaagct gatgcaataa attacttgcc aaacctaaat
     4681 cgttatcatg gacccgaaaa aaaaatacac tgactctggt atagaaataa aatctgtata
     4741 tacccatgat gatctgccag caaccaaatc tgaaaacccg ggtgaattcc cttttaccag
     4801 aggtgttcaa cccgacatgt accggggcaa actctggacg atgcgtcaat acgccggctt
     4861 ttctacagct gaagaaagta ataaacgcta tcactattta ttatcacagg gcgtcagtgg
     4921 cctgagtgtg gcttttgatc tgcctacaca gatcggttat gacagtgacc atccattggc
     4981 tgaaggtgaa gttggtaaag tgggagttgc tattgattct atcgaggata ttcaaacctt
     5041 attcaatggt ataaaattgg aagacgtcag cacttccatg accatcaatg cgacaggctt
     5101 tatacttctt tcgttttatg tagcactggc caaacagcag ggcgcagacc ttaaaaaaat
     5161 atccggtact atacagaatg acatcttaaa agaatatgct gccaggggta cctatatcta
     5221 ccctcccaag ccatccatgc gaatcataac agatatattt gaatggtgca gtaacgaagt
     5281 tcccaaatgg aatactattt ccatctccgg gtatcatatc cgtgaggccg gcagtacggc
     5341 tgttcaggaa atagccttta cgctcagcaa tggtaaagcc tatgtagaag ctgccctgaa
     5401 gaaaggtttg gatataaacg tttttggaaa acgactctct ttctttttca atgcccacaa
     5461 taacttattt gaagaagtag ccaagttcag ggcagccagg aaaatgtggg ccaaaataat
     5521 gaaagacctg ggtgctactg atcctaaagc gatgatgctg cggttccata cacaaactgg
     5581 tggcagcacc ctgactgcac aacaaccctt aaataacata tccagggtga ctatccagac
     5641 tttagctgct gtactgggtg gcacacagtc gctgcatacc aacggatacg atgaagcgtt
     5701 aagcctgccc accgaggaag ccgccaagat tgcattaagg acgcaacaga tcgttgcctt
     5761 tgaaagtggt gccccggatt cagttgatcc cctggccggc tccttcttta ttgaatccct
     5821 taccagggag gttgaagaag cggcctggag attaattgaa aaaattgatt cactgggtgg
     5881 tagcgtaccg gcaattgaac agggatttat acaaaatgaa attgcccgca gtgcttacga
     5941 ataccaaaaa aatattgaaa ccggtgaaaa gatcattgtt ggtgtcaata aatttcaaac
     6001 ggaagatcat cataaaatcc ccatcttaaa agtagatgac agtatcagaa ccttacaaat
     6061 agaaaagctg gaaaaactca ggaataaccg caatcatgca aaatgtgacc agttactgca
     6121 aatattaaac gacaaggtaa ccagcgggga aaacataatg cctgctgtag tagaggcagt
     6181 tgagaataaa tgtaccttag gtgaaatcgc agataccctg agagaagtct atggagaata
     6241 taaataattg acaaatactg ttaataggat atcttaatta tttggcttac cataaaaaac
     6301 tacctgtttt ttaaatattc tcttacaatc actgattaat aaatctgtta tgaagcatac
     6361 aagattaatt accatctgcc tgctgctcct tatttcctcg ttaagtaatg cacagcagcc
     6421 ttctttcgtc tctgacagcc ttgatagtta tatagaaaga ggaatgaaac aatggcaaat
     6481 acccggctta gctattgcca tcgtaaagaa cggaaaagtg gtccttagta aaggttatgg
     6541 tgtccgtgaa ttgggtaaag atgataaagt ggatgaaaac acccttttta ttattgcttc
     6601 caattcaaaa ctatttaccg gaactgctat cgccaagctc gatcatgaga aaaaattatc
     6661 cctgaatgat aaggtcacac aatatatacc ctggttcagg ttatatgatt ccacctctgg
     6721 caaactggcc aatataagag atatgctttg tcaccggctt ggcaccaaaa cattccaggg
     6781 cgattttact ttctgggatt caaatcttcc aaaagattcc ataatctgga agatgcgtta
     6841 cctgaagcca acgggagagt tcaggcagga ttatggctat tgcaattcag cattccttgt
     6901 agccggtgaa atacttacca aagtaacagg gatgagctgg gaaaattatg tgcaggaaaa
     6961 tatgttaacc ccgcttggca tgcgtaatac ttacatgaat acaaccgggt tagccaaccg
     7021 ggtaaatgtg gcggctccgc ataacaacct cttcagttcc attactaaga ttccttttga
     7081 taatgttgat aatctcgggc cggctaccag catggtcagt aatgttaacg acctgaccaa
     7141 atggctatta cttcaattgg acagtggtaa atatatgggc aaacaggttt taccgtgggc
     7201 agtactgcag aaaacaagag atatcaatat tataaccgga agcaggaaat caactgttta
     7261 tcccacacat ttccgcggct atggtcttgg agtttattca acagattatg caggcaaaca
     7321 ggtttactgg catacaggtg gtgcttttgg gcatgtaacc aatgtttgtt ttgtcccgga
     7381 agaaaacttg ggtattacta ttctcaccaa caacgacaac cagagtttct ttgaagcgtt
     7441 gcgttaccag ctactggacg cttaccttgg agtgtcttat gcggacagaa gtaaatttca
     7501 gtgggggttc tttgaacagg gtaaaaagaa actggatgaa gaactatcgg ctttacagga
     7561 aaaagtaaat aaaaaaaatc tgccggaaat aaagcccgaa aattttaccg gtgattattt
     7621 taatacggtt tatgggaaaa taacaatcac taaaagcggc aatatgctaa tatgccgttt
     7681 tcagcatcac cccgacctga tcgggtacat ggaatacatg gacaataatg aataccggct
     7741 aacctattcc aatattggct atggtattta tccggctaaa ttttcaataa aagacgggaa
     7801 agtaattgcc gtagagatca aggccaatga ctttgtagaa agtgatgcct atatttttgt
     7861 gaaagactcc ttagatggag tatcaaaata aacggcattg atccaacaat aaaaagcggg
     7921 atgaagttcc catcccgcca tccaatacct gcaagtcaga aattgcctac atccacttgc
     7981 tcaggtactc attgaattcg tgtcgtaaaa tattcataac cctttcaaaa tcttcatatt
     8041 tttcatgcag agccgtatgt tcagcaatca ttgcagtttc catacgtccg ccatgttgct
     8101 gggcatcttt tcctaatttt tcaacataca ggttcacttc atgtttcaat tcatcaatat
     8161 tattccgctg aacaataaac tggttttgaa aatgttcaac cccctgccgt gcttcaaatg
     8221 tcgtattctt caaggcaact tctgataaac ggctctccat tatatcaagc tcatctttat
     8281 aaaatccaaa gcctttttgc cagatagtat gatcggttcc tattcgggta atatttgtat
     8341 agctcatatt tgatttcttt taatcaaatt tattttcact tttcattaaa tcaactgacc
     8401 atagtcattc cagaacatga ttgatttcag aaatggaggc aaccctgtac agattatgat
     8461 cctgtatcca ctatgtgaat tgtttgtgga aacacctgaa aaagataaag cgtcttatca
     8521 gacgctttaa agatttcact taattattgt ttgtatttac attaattaac tcttctttac
     8581 ttctttagcc catgcatctt ttttttcaac agttttgttg aataccagtt taccaggcgc
     8641 agattcttta tccagtacaa agtatccctt tcggataaat tgataacgtt cttcaagtgt
     8701 agcatttttc aatacaggtt cagcatatgc tgtggacaaa atttccaatg atccggggtt
     8761 caggtattct ttaaaatcgc cttcttcatc tgtcggatct tctactttaa aaagacggtc
     8821 atatagacga acttctgcat tgattgcatg ctttacactc acccagtgca atgttccctt
     8881 cacattgata ccgcttgtat ccgaaccact tttactttca ggaatatagg tgcaacgaac
     8941 ttctgcaata gtaccggcag catcttttat cacttcgtca catttaataa tataagcact
     9001 tttaagacga accatctgac ccggtgctaa ccgaaaatat ttctttgggg gattttccat
     9061 aaaatcctcc ttttcgatat acagttcagc actaaacggg atttcccttg tccccccctt
     9121 ttcatcttca ggattatttt cactatgcaa tatttcttcc tccccggtat agtttgttat
     9181 caccactttc aatggatcaa aaacaaccat tcttcgcaga gctatcctgt ttaaatgttc
     9241 cctgacacaa aactcaagca acccgacatc caccatattt tcccgtttgg ccactcctat
     9301 cctgtcacaa aactcacgga tgctatccgg tgtatacccc ctgcgtcgta atccactgat
     9361 ggtcggcatc ctaggatcat cccagccagt aacatggttt tcatttacta attgcagcag
     9421 ttttcttttg ctggtaactg tatagttgat attccttctg gcaaactcat actggcggga
     9481 cggatagatt tctaattttt caataagcca gtcgtataac tcccggtggg gtacaaactc
     9541 cagggtacag atagaatgtg ttatttcttc aatcgaatca ctttgaccat gtgcgaaatc
     9601 atacatcggg tatatgcacc atttatcacc tgtacgatgg tgatgagcat gcttgatccg
     9661 gtacagaatg ggatcccgca tgagcatatt gatatgtgcc atatcgatct tcgcccttaa
     9721 cgtcctgctt ccgtccgggt actccccatt tttcatccgt acaaacagat ccctgttctc
     9781 ggcaatcgtc ctgtcccggt aaatactatc ctttcccggt tcggtaggcg atcctttcat
     9841 taccgccatt tcttcagccg tactatcatc aacatatgca aggcccttgt cgatcagttt
     9901 taaggcgtac tcatataaag tgtcgaagta atcgcttgca aatcgttcat gcttccactc
     9961 aaatccaagc cattttacat cttcctttat actttctaca tactccgttt cctctgttac
    10021 cgggttggta tcatcaaacc tgagattggt atatccggga tacttttggg taagcccaaa
    10081 gttcaggcag atgcttgatg catggcctat atgcaggtaa ccattgggtt ccgggggaaa
    10141 tcgggttaca atacttttat acttcccgtt tttgaggtct tcttcaatta tttcctcgag
    10201 aaaattcaaa ctgcgttctt ctgacataac tcttttaaaa taaagcataa agatacgaat
    10261 tctgaagaga gtataaggcc tgtttatatc tgcataaaga ggtaaattgt atagcaaacg
    10321 cagcaaatga acaacgaggc tatttataaa aaaacacagc tttatacggg cgaccaaccc
    10381 ttagaataaa aaatcactaa aaatcagcat gatcaatgag gtcagtcata gatattgccc
    10441 tctaataacg gcaaattgcc tcaagtaaaa cttacaaccg atgaaaaaaa tccttgtccc
    10501 aacagacttt tctctcaatg cagacagagc catagattat gctgtgcaaa tagcaaaatt
    10561 gaacgaggca acaatttatc ttatccatgc ctgcgatcat ttagatactt tatacatgga
    10621 aagtgcaatc ccggagagag aatacaataa ggcaataacc gatgcggcat ttgataaact
    10681 cgaaattctg agaagaagca ttgaagagac agaacaggta cttgtaaaca tacaacttta
    10741 cagcggccca attaatgata caatacttgt ggctgccgaa gaacataatg tggacctgat
    10801 cataatgggt acgttgggta tttccggaat aaaagataaa atattcggaa gccgaactgc
    10861 atcagtaatc agtaaaagca ttgttccggt attagcaata ccgcttgagt acgattggag
    10921 tccgcctgca aaactgttgc ttgccatcaa tcgttttgat gaagtatctg accttctcaa
    10981 ccccgtcttt gacatggcaa ctatttttaa tgcggaagta aacataactg tgtttacgga
    11041 tgaggataat gcagcggctt ccgactacct ggaagatgca aaagaagtga agttagcaga
    11101 ggaaaaactg agaagaaaat tcagcaagct ggtaataaaa gcggagcatt tatccggccg
    11161 ctatttcgaa gacagtataa atgaacatat aagtaaaaac aaaatcgatt tgctggcaat
    11221 gacaacccat aaaagaagtt ttatcggcag cattttcaac aggagcatca cccggaagat
    11281 gagctatcat tcaaaagttc ccatactggc tataccggtt aaataattga tgggtatcca
    11341 taaacacata ttgacatgaa aacaatactg attccattcg atttctcagc aaatgccagg
    11401 aaggcacttg cttatgcact ggaactggga aagctatttg agtcacagtt atacatttat
    11461 catgttttgc atgaatcacc ctatacactt gcagctgctg caactgaaca ggaaatggaa
    11521 aaattaatag cgaaagacgg ggaagaaaaa acagcagccc ttaaagatga agtgaatact
    11581 cttttacaat cattacctat caccttctct gcggaaagaa tcaaaacaaa agcggaatat
    11641 aacccactgg tggttgaaaa aataatagct gtagcaaaag agcaggacgc cgaactggtt
    11701 ataatgggta ctcatggcac ttccggcatt aaaaaatttt tctttgggtc caacacatcc
    11761 aatatgatta ccaaatcgga tatacctgtt ctggccattc ctgaacagta tgaatacact
    11821 ccggtaaaaa caattcttta tgcctcagat ttagaaaatc ttccggcgga attaaagaga
    11881 attattcctt ttgcccatag cctgcatgca ggaattaatg tacttcattt tgattatggc
    11941 cagaatgaaa gaaaaataac agaggaagag gcaaacatca taattaagaa taatcaatac
    12001 cagcatataa aattcgtaaa agaaaaagcc gatctgacta tccctctttt aaaac
//
LOCUS       DAORTI010000110        11968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5433_length_11968_cov_7.740871, whole genome shotgun sequence.
ACCESSION   DAORTI010000110 DAORTI010000000
VERSION     DAORTI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5433_length_11968_cov_7.740871"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="PLO70_12640"
     CDS             <1..150
                     /locus_tag="PLO70_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75359.1"
                     /translation="PFFTTKPTGQGTGLGLSLSYDIVTKGHGGELKAATKEGEGSRFT
                     IILPA"
     gene            167..544
                     /locus_tag="PLO70_12645"
     CDS             167..544
                     /locus_tag="PLO70_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ75360.1"
                     /translation="MKVLVVDDEKDVQVLFEQRFRKEIKSGEMMLSFAFSGEEALQYM
                     KANEHEAVLILSDINMPGMSGLELLQHIKEKYEKPPPVVMMITAYGDDENYNQAMKLG
                     ADDFLTKPVDFNSLKEKLKTRIA"
     gene            541..>1166
                     /locus_tag="PLO70_12650"
     CDS             541..>1166
                     /locus_tag="PLO70_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQZ75361.1"
                     /translation="MRKILVADDEADLEILIRQKFRQKIREQQYEFVFAINGQDALEK
                     MQLHPDVSIVLSDINMPEMDGLTLLTRLGESNPLTKTVMVSAYGDMDNIRTAMNRGAF
                     DFVTKPVNFEDLELTMEKTIRHVDQMKETLQAIKENNILKMYVDENVLSFMGSREYEA
                     SLMANETVEATVVFIDICSFTAISETEKPDTVVSLLNTYFDLMVKEIIA"
     assembly_gap    1167..1266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1267..2735
                     /locus_tag="PLO70_12655"
     CDS             <1267..2735
                     /locus_tag="PLO70_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQZ75362.1"
                     /translation="GTMQYQQVYLFLIKKLETGLPAYLTYHNADHTRSVIEAAENIGK
                     AENVSGDDLVLLKTAALFHDAGFLQHHQNHEELSCEIARDHLPDFEYTPEQIDAICRI
                     IMATRLPQNPQDHLAEILCDADLYYVGSDQYAATAEKLFKEWKKTRTIKTQTDWILQQ
                     VEFLSLHQFFTQTAIRERTEQKQKNVQELQLKLEGQLTHTNKINLTEMVQDLLLMIAG
                     VVMAGFALKGFLVPNHFFDGGITGISLLIHEIYHVNLAIVIILLNLPLIIISYFSVGS
                     RFAYRTLVSVILLGICLFLLPDLDLTSDKLLISIFGGVFLGIGIGLIMRVGAALDGIE
                     VLALYTLKRTSFTITEIILGINILIFSIAAFRFGIETALYSILTYFAATRSIDYVVEG
                     LQAFTGVTIISGKSEVIKYQLVNKLGRGITIYKGERGFLPGKFEVSSDCDIIFTVVSR
                     LEMRKLKNLVYDVDPKAFVFANTIKEASGGIIKRRRQH"
     gene            2937..5132
                     /locus_tag="PLO70_12660"
     CDS             2937..5132
                     /locus_tag="PLO70_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQZ75363.1"
                     /translation="MSQSRQLGAIMFADIMGFTAMMEENEAAASLCRSRLRNALKSAC
                     TSWNGRIVELTGDGGLCIFQSAYDAVRCAIAVQAEMKTADTIPLRIGIHSGDVVIDDN
                     SVYGSVVNIASRVESFAVAGAIFITAKIFDEIKNQPDIQVQQMGRFKFKNVEEPVLIY
                     AVSNPGITVPPVHQLQGKGHEVAEKKFISLQKSLIALAAIIGLAVFIYFRFLKQEGPL
                     KLKSIAVMPFINQGNNPDNEFFSDGITEDIHTHLAKINSLNVIAHSAMMLYKNSEKSI
                     KQIGKELNVSTILKGSVRRVGDKIRIYAELIDAGTSKNLWAETFDREYAKIFDIQSEI
                     AQSIAQKMAINISAAEKNLIRQKPTDNLTAYENYLKGRSHYYEYKKEENEKAIEYFKK
                     AIALDNNYALAWAGLGDAFSQKNQRFGYELSWNDSSKIAGLRAVSLDSNSSEAYKALA
                     NAYYSGGEYDKGFELLRRSVALNPNNEQAVGNLGAGYFLRAQLVEALRWEKKAAAINP
                     KNGIPYQIIGWIYRLLGDYNNAEQWLKKSLALKIFSDTYRELGYTYLLQQKKDSAKLL
                     VPAIIAIDSTNARNHETAGVLLQMAGDNQKAREHFQKAIDLNKSISNDKDAIAPIGLG
                     QLLLEMNNKIDAEVQLSNSLSVFLKTIEEGSQDDDPPLNLAAIYAIKNDKQEAIKWFN
                     KAVSANWLDYGFTDICPWFDNIRNEPAYKQQVTTVKKKIEVMRKNAAVY"
     gene            5200..6783
                     /locus_tag="PLO70_12665"
     CDS             5200..6783
                     /locus_tag="PLO70_12665"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HQZ75364.1"
                     /translation="MKWILLFLFYPVAAFTQQPSCDLLITNGKIIDGTGNNWYYGSMA
                     IKDGKIIGIGRNINYAAARTIDARGLIVAPGFIDVHTHLEGDEARDPTAKSFIYDGVT
                     TCVTGNCGSSNVNIGKYLHWVDSLKLSINVATLVGHNDVRKAVMGRANRDASAEEMLR
                     MENIVDQAMKDGAVGLSTGLIYIPGTYSKTPEIISLAKVTAKYNGLYASHMRDEGDSV
                     TQAIEEALTIGREARIPVEISHFKLSGQQNWGRSKITVAMIEAARREGIEVTIDQYPY
                     TASSTSISTLIPDEILADGQDSIKARLQRPDIRKYVISSMLQRLKKRKLKHFGYAVVA
                     YHAADTTYNGKSIEQINLMKGRKHKARYEAETVIDIMIAGGASAVFHGMGEEDVKRIM
                     QYPFNMFASDATIRVLNAGMPHPRGYGTNARVLGKYVREEKILSLEEAIRRMTSLPAQ
                     KFQLKDRGLLREGYAADIVIFDEKEVKDISTFEKPHAYSKGFHHVIVNGVLTVENEKH
                     LGIRAGKALFGPGASLHSN"
     gene            6801..>7788
                     /locus_tag="PLO70_12670"
     CDS             6801..>7788
                     /locus_tag="PLO70_12670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ75365.1"
                     /translation="MRKLLIILSLLPGFVVAQQSVTAFYDSLLQQQASQGFSGVLLVA
                     EKGAIIYNKAVGYREFAGKITLQESAIFEMASVSKQFTAMIIMMLKEKGKLNYDDAVE
                     KYLTIPYKGITIRHLLTHTSGLPDYQDIMDKFWDKTKVAGNNDCIAYLNKYAPPKSFE
                     PGDRFEYSNTGYLLLASIAEKASGKDFIQLCRQWIFRPLKMKSTNIRTLAEKAATKNF
                     ALGHIYVKEKDQYIRADSFPSSDYTLWLGNRKGPGRISSTAADLLKWDKALYTEKLVK
                     QATMEEAYAPMKLNNGSYSNYGFGWMLQTDPVIGKFVYHTGDNPGYKTMIIRY"
     assembly_gap    7789..7888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7889..9019
                     /locus_tag="PLO70_12675"
     CDS             <7889..9019
                     /locus_tag="PLO70_12675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ75366.1"
                     /translation="EKGWMQGGTALIIRNGKIVYYKAAGYNDLDSRTPMQKEHIFRIA
                     SQTKAITSVAIMILFEEGKLLLDDPVSKYIPAFKKQQVLDKFNPSDTTYTTTPAKKEI
                     TIRELLTHTSGLGYAQIGSKEANAIYAKHDLTAGLGVQEDKLLEAMNRLAKLPLMHQP
                     GEKWTYGLNTDLLGCLAEVISGMSLDEFFRKRIFEPLGMKDTYFLVPKEKATRLVNLY
                     REDSTGKLVKATGNMLNGKTITPNYPLEASTYYSGGAGLSSTIYDYAVFLQMLLNNGM
                     YNGKRILSRNAVRMMTMNQIGDLSRGDDKFGLGFQVVTERGSARTPAQAGTFSWGGAF
                     ATSYWVDPKEKMVMLFYRQLQNTTKGEVVEKFRALTYAAINN"
     gene            complement(9097..9705)
                     /locus_tag="PLO70_12680"
     CDS             complement(9097..9705)
                     /locus_tag="PLO70_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75367.1"
                     /translation="MNKVFALLFFVVAAMGMYSCTSKLYPVQYYEQHKATIHEIETLY
                     KKITASRLLSVAFYDLGFSDISLEFKTDSVRYIYDFAYHESRINDSLARFGFDIAMVQ
                     KMIGDMRRIGCSWINNLDYYVDGKKQYLVFMSVPVKQFSLFPVLQKNKYYLFNFYAQP
                     QYYDEQGRLLDKKKLQQLRKINSEVFRRINEKVCYTISGKFR"
     gene            complement(9698..10603)
                     /locus_tag="PLO70_12685"
     CDS             complement(9698..10603)
                     /locus_tag="PLO70_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75368.1"
                     /translation="MKKGLLILFVTISLAAGAQKKRVPQKEINEAGRIRALELVAGER
                     NTLEQSSFWPHITPSAFLDNLKRNIEYPLKINNGRSTNFCAFGAITYTCLKNEPFRYT
                     ISMLNLYKNGESYYRLATLRPSQTIKDAAGLMMFQGDLDIQPADQMWFLCLANRFKGY
                     LNFFNRKYDKGDENTMWAATNLAKFNRMLRKMCKYKVQSKGSDLLKPKINNLPDFLEQ
                     KLNAGEVYLYLNNAILRKKNHNRIKKRIPTHYVALTGIEKNNDRIVIKYWDGGYKTLK
                     EVTLHSLKQIIYGVSWVNYKEENNE"
     gene            10778..10939
                     /locus_tag="PLO70_12690"
     CDS             10778..10939
                     /locus_tag="PLO70_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75369.1"
                     /translation="MSDEAKRITGASGKSKHCSPVAKLFLFNRQLQKTDHGAMVEYYR
                     PLTYQAIND"
     gene            10959..>11968
                     /locus_tag="PLO70_12695"
     CDS             10959..>11968
                     /locus_tag="PLO70_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ75370.1"
                     /translation="MKILWTYLKPFQWWIILALTLAGIAQILSLYDPVIFGKIIDNYA
                     LNPGNKTEDELVKGVSFWLGIAIAIALAARLVMVFKDYVIRLIVQRFGMQIFNDGLRQ
                     TLRLPYQDFEDQSSGEILSILQKVRTDTERFITNFINILFSSFVGIGFLIWYAVTKHW
                     ALIPVFLIGVVLLGGLTSILSRSIKVLQRSLVRQNRQMSGTITESLRNIELVKSLGLT
                     YPEIRRLKQHTRDIYDLEMKKVKKVRTLTFFQGAILSFLKQSILFILLWLIFRKVLSP
                     GELISMQFISLGIIGPLQDLGSIILSYREAEASLQLFNELMQKEPEYRPEEPIDVGEI
                     EHL"
BASE COUNT         3626 a         2302 c         2538 g         3302 t
ORIGIN      
        1 cctttcttta ccaccaaacc aacgggacag ggaacgggat taggattatc tttaagctac
       61 gacattgtta ccaagggtca tggaggggag ctgaaagcag caacaaagga aggtgaaggc
      121 agccgtttca ctattatttt accagcataa aaattaaatt taatctatga aggttctagt
      181 tgttgatgac gaaaaagatg tgcaggtgct gtttgaacag cgcttcagaa aagagataaa
      241 aagtggtgaa atgatgcttt cttttgcctt ttccggtgaa gaagcattgc agtacatgaa
      301 agcgaacgaa catgaggccg tattaattct ttcggatatt aatatgcctg gcatgagcgg
      361 ccttgaactg ctgcagcata taaaagaaaa atatgaaaag ccaccaccgg ttgttatgat
      421 gatcactgcc tatggtgatg acgagaacta taaccaggcg atgaaactgg gtgctgatga
      481 ttttctgacc aaaccggttg attttaattc cctgaaagaa aaacttaaaa ccaggattgc
      541 atgagaaaaa ttttagtggc tgatgatgag gcagatctgg aaatacttat ccggcagaag
      601 tttcgccaga aaataagaga gcagcagtac gaatttgttt ttgcgatcaa tggccaggat
      661 gcactggaaa agatgcaact gcatcccgat gtatccatcg ttttgagcga tattaatatg
      721 ccggaaatgg atggcctgac cctgcttacc cggttgggtg aatccaaccc gcttaccaaa
      781 accgtgatgg tgtctgcata tggcgatatg gataatatcc ggaccgccat gaaccggggt
      841 gcttttgatt ttgtgaccaa accagtgaat tttgaggacc tggagctgac catggaaaaa
      901 acgatccgcc atgtagacca gatgaaggaa acattacagg ccatcaaaga aaataatata
      961 ctgaaaatgt atgtggatga aaatgtgctg agctttatgg gcagccgtga atatgaagca
     1021 tccctgatgg ctaatgagac agtggaagcc actgtagtgt ttattgacat ctgcagtttt
     1081 actgccatca gcgagacaga aaaaccggat actgttgtgt ctttattaaa tacctatttc
     1141 gatctgatgg taaaagaaat cattgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnccgg aaccatgcaa tatcaacaag tgtacctgtt tctgataaaa aaattagaaa
     1321 ccggtttgcc tgcttacctt acttatcata atgcggacca taccaggagt gtgattgaag
     1381 cagcggagaa tattggtaag gcggagaacg tttccggcga tgacctggtt ttgctgaaga
     1441 cagcagcatt atttcatgat gcaggttttt tacagcatca tcagaatcac gaagagcttt
     1501 cctgtgaaat tgcaagggat cacctccccg actttgagta tacaccagaa caaattgatg
     1561 cgatctgccg gatcattatg gctacccggc ttccgcaaaa cccgcaggat catttggcag
     1621 aaattctttg tgatgcggac ctctattatg ttggctccga tcaatatgct gccacagcag
     1681 aaaaactgtt taaagaatgg aaaaagaccc gtactataaa aacacaaaca gactggatac
     1741 tgcagcaagt ggagttctta tccctgcatc aattttttac acaaacggct atccgggaaa
     1801 gaacggaaca aaaacaaaaa aacgtacagg agctgcaact caaattagaa ggacagctta
     1861 ctcatacaaa caaaattaac ctgaccgaaa tggttcagga tcttctttta atgattgccg
     1921 gtgttgttat ggccgggttt gcattaaaag gatttcttgt acccaatcac ttttttgacg
     1981 ggggtattac cggtatttca ttgctgattc atgaaatata tcatgtcaac ctggcgattg
     2041 taataattct gctgaacctt ccgctgatta tcatcagtta tttttctgtg ggcagcaggt
     2101 ttgcatacag aacattggtc agtgttatac tgctgggcat ttgcttattc ctgcttcctg
     2161 atcttgattt aacctctgat aaattactca tctccatctt tggaggagta ttcctcggaa
     2221 taggtatcgg gctgatcatg agagtggggg ctgccctgga tggtattgaa gtactggctt
     2281 tgtatacact gaaacgaacc tcttttacca tcactgaaat cattcttggg ataaacatcc
     2341 tcattttcag tattgccgct tttagattcg ggattgaaac agccctgtat tccatattga
     2401 cctactttgc agctacccgc agtatagatt atgtagtgga aggtttgcag gcctttactg
     2461 gtgtcaccat catttccggg aaaagcgaag tgatcaaata ccagctggta aataaactgg
     2521 gaaggggtat tactatatat aaaggggaac ggggcttttt gccgggtaag tttgaggtca
     2581 gttcagactg tgatatcatc ttcaccgttg tttcaagatt agaaatgcgc aaattgaaaa
     2641 atcttgtgta tgatgtagac ccaaaagctt ttgtttttgc caatactata aaggaagctt
     2701 ccggcggtat tatcaaacgg agacgccagc attaacagta actgaaatca tctgttcttg
     2761 tttacaattg ttccgcggat tatgtattgg ctatccgctt cgcctgcttt tagcgtaata
     2821 aaccaactgc actccacccc tgctataaac taccgggtat attgtttttt attttctgta
     2881 aattgacaag gcattgtaac aaaagatagc tacccgttca gccatcaata taaaatatga
     2941 gccagtcccg ccagctaggt gcaattatgt ttgccgacat catgggcttt acggctatga
     3001 tggaggaaaa tgaagctgcg gccagccttt gccgcagccg tttacggaat gcgttgaaat
     3061 cagcctgcac ttcgtggaat ggccgtatcg tggaacttac cggtgatggt ggcctctgta
     3121 tattccaaag tgcatatgat gctgtacgtt gtgccattgc tgtacaagca gaaatgaaaa
     3181 cagcagatac cattccgctg cgtatcggaa tacattccgg cgatgtggtg atagatgaca
     3241 atagtgtcta tggcagtgtg gtcaatattg cgtcccgggt agaatcattt gcagtagccg
     3301 gagccatctt tatcaccgcc aaaatctttg atgaaataaa gaaccagccg gatatacagg
     3361 tacagcaaat gggacgtttt aaattcaaaa atgtagaaga acctgtattg atttatgctg
     3421 tcagcaaccc gggtattact gttcctccag tacaccagtt acaggggaag ggtcatgaag
     3481 tagctgaaaa gaaattcata tcgctccaaa aatctctgat tgcccttgca gcaatcattg
     3541 ggcttgctgt ttttatatat ttcaggtttt taaaacagga agggccgtta aagctgaaat
     3601 ccattgctgt aatgccattt atcaaccagg gcaataaccc ggataacgaa tttttctctg
     3661 atggcattac cgaagatatt catacacacc tggcaaagat caactcgttg aatgtgattg
     3721 cacattctgc tatgatgctg tataaaaatt cagaaaaatc aataaaacag attggcaaag
     3781 aattaaatgt atctaccata ctaaaaggaa gtgttcgccg ggttggagat aaaatccgca
     3841 tctatgcaga attgatagat gcaggaacct caaaaaatct ctgggcagag acatttgacc
     3901 gtgagtatgc taagatattt gatatacaaa gtgagatcgc ccagtcaatc gcccaaaaaa
     3961 tggcgataaa tatttcagca gctgaaaaaa accttatccg ccaaaaacct acagacaacc
     4021 ttacggctta tgaaaattac ctgaaaggcc gcagtcatta ttatgagtat aaaaaagaag
     4081 agaatgaaaa agcgattgag tattttaaaa aagcaattgc cttagataat aattatgcac
     4141 tggcatgggc cggattgggt gatgccttca gccaaaagaa ccagcggttt gggtatgaat
     4201 tatcctggaa cgattccagc aaaatagccg ggttaagggc tgtttcgctg gactctaatt
     4261 catcagaagc atataaggct ttggcaaatg cttactattc cggaggcgaa tatgataagg
     4321 gctttgaact gctccggcga tctgtagctc ttaatccaaa taatgaacag gctgtgggta
     4381 atcttggcgc cggttatttt ctgcgggcac agctggttga agcgttgcgc tgggaaaaaa
     4441 aggccgctgc tatcaatccc aaaaatggta tcccttacca gatcattggc tggatctacc
     4501 gtttgctggg cgattataac aacgctgaac aatggcttaa aaagtcgtta gcattaaaaa
     4561 tattttctga cacttaccgg gaacttggtt atacctattt actgcaacaa aaaaaggact
     4621 cagcgaaact gctggtgcct gcaattatcg ctattgacag taccaatgcc cgtaatcatg
     4681 aaacagcagg tgtccttttg caaatggcag gtgataacca aaaggcgagg gaacattttc
     4741 aaaaagctat tgacctgaac aaatcaattt ccaatgataa agatgcaata gccccaatcg
     4801 ggcttgggca actgctgctg gaaatgaata ataaaataga tgctgaagtt caactctcta
     4861 atagcctgtc cgtttttctg aaaacgatcg aggagggttc acaggacgat gacccgccac
     4921 ttaacctggc tgccatttat gccataaaaa atgataaaca agaggccatt aaatggttca
     4981 ataaagctgt aagcgccaat tggctggact atggtttcac tgatatatgt ccctggtttg
     5041 ataatatccg gaatgaacca gcctataagc agcaggtgac caccgtgaaa aaaaagatag
     5101 aggtcatgag aaaaaatgct gctgtctatt gattatattt tacaaaattt atataaatca
     5161 ccaacttcat cccttccaaa ctttcagtaa cttgtcggaa tgaaatggat ccttttattc
     5221 ctgttttatc ctgtagcggc ctttacccag caaccttcct gcgatttatt aatcaccaat
     5281 ggaaagatca ttgatggcac cggtaacaac tggtattacg gcagtatggc tattaaggat
     5341 ggaaaaatta tcggcattgg cagaaatatt aattatgcag ctgccaggac catagatgca
     5401 agaggattga tcgttgcgcc gggtttcatt gatgtacaca ctcacctgga aggtgatgaa
     5461 gccagggacc caaccgctaa aagttttatc tatgatggtg ttaccacctg tgtaaccggc
     5521 aattgtggtt cttccaatgt aaatattggt aaatacctgc attgggttga ttcattaaaa
     5581 ttatctatta acgtagccac gttagtggga cataatgatg tccggaaggc tgtgatgggt
     5641 cgtgccaacc gggatgcctc ggcagaagaa atgttgcgga tggaaaatat cgttgaccag
     5701 gcaatgaaag atggtgctgt tggtttatca accggtctta tttatatccc cggcacttat
     5761 agtaaaacac cagagatcat ttcactggca aaagtgactg caaaatataa cggactttac
     5821 gcctcacata tgcgggatga aggtgatagt gtgacacaag ccattgaaga agcactgaca
     5881 atcggaagag aagccaggat accagtggag atatcacatt ttaaattgag tggtcagcaa
     5941 aactggggaa gaagtaaaat aaccgttgcc atgatagaag cagcaagaag agaaggcatt
     6001 gaggtaacaa tcgaccaata cccctatact gccagcagca cttctatcag cacattgata
     6061 cctgatgaaa ttctcgccga tgggcaggac tccattaaag ccaggttgca acggccagac
     6121 atacgaaagt atgtgatcag ttctatgctc cagcggctta aaaaaagaaa gcttaaacat
     6181 tttggttatg ccgtcgtggc ttatcatgct gcagacacca cttataatgg taaaagcatt
     6241 gaacagatca atttaatgaa aggaagaaaa cataaagccc ggtatgaagc agaaacagtg
     6301 atcgatatca tgattgccgg aggcgccagt gccgtctttc atggtatggg agaagaagat
     6361 gtaaaacgca tcatgcaata tcctttcaat atgtttgcca gtgatgccac catcagggta
     6421 ttaaatgcag gcatgccgca tccccgcggc tatggtacca atgcaagagt gttgggaaaa
     6481 tatgtgcggg aagaaaaaat actctctttg gaagaagcta tccgcagaat gacaagttta
     6541 ccggcacaga aatttcaatt aaaagaccgt ggattattaa gagaaggcta tgctgcagac
     6601 atcgtcatat ttgatgaaaa agaagtaaaa gatatttcta cgtttgaaaa gccacatgcc
     6661 tactctaaag ggtttcacca tgtgatagtg aatggtgtac tgacagtgga aaatgaaaaa
     6721 catttaggga tccgggccgg taaagctttg ttcgggccgg gtgcatccct tcatagtaat
     6781 taaattaata taaatagtgc atgcggaaat tgctgatcat cctgagttta ttgccgggat
     6841 ttgttgtagc acaacaatcc gtcactgcat tctatgacag cctgctgcaa cagcaggcat
     6901 cacaaggctt cagcggtgta ttactggtag cagaaaaagg agccattatc tataataaag
     6961 cagttggtta tcgtgagttt gccggtaaaa tcactttaca ggaatcagct atttttgaaa
     7021 tggcctcggt ttccaaacaa ttcaccgcca tgatcatcat gatgctgaaa gaaaaaggaa
     7081 agctgaatta cgatgatgct gttgaaaaat atcttaccat cccttataaa ggaatcacca
     7141 tccgtcattt actgacgcat accagtggcc tgcctgatta ccaggatata atggacaaat
     7201 tctgggataa aacaaaagtg gctggtaata atgattgtat tgcatacctt aataaatatg
     7261 ctccgccaaa aagttttgaa ccgggtgaca gatttgaata cagcaacacc ggctatttat
     7321 tattagccag tattgcagaa aaagcaagcg gaaaagattt tattcaatta tgccgtcaat
     7381 ggatcttccg tccgctgaaa atgaaaagta ccaatatcag gaccttagcg gaaaaagccg
     7441 cgacaaagaa ctttgccctc gggcatatct atgtgaaaga aaaagatcag tatatccggg
     7501 cagattcatt tccatcctct gattataccc tctggctggg caatcgtaaa ggtcccggcc
     7561 gcatcagctc cacggcagct gatctgttga aatgggataa agcgttgtat acagaaaagc
     7621 tggtgaaaca agccaccatg gaggaagcct atgcccctat gaaactgaat aatggcagtt
     7681 attccaatta tggttttggc tggatgttgc aaacagatcc ggtgattgga aaatttgtct
     7741 atcacaccgg cgataatccg ggttataaaa caatgatcat acgatatcnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaaaggctgg atgcagggtg gcacagcttt
     7921 gatcatccgt aatggaaaga tcgtatacta taaagcagcc ggttataatg atcttgacag
     7981 cagaacgccg atgcagaaag agcatatatt cagaatcgca tcacagacta aagcgatcac
     8041 cagtgtggcc atcatgatcc tgtttgaaga gggaaagctt ttgctggatg atccggtttc
     8101 caaatacatt cctgctttta aaaaacaaca ggtactggat aagttcaatc catcagatac
     8161 tacttatacc actacacctg ccaaaaagga aattaccatc agggaattac tgactcatac
     8221 ttccggatta ggctatgcac agatcggctc taaagaagcc aatgctattt atgccaaaca
     8281 tgatcttacc gccgggttag gtgtgcagga ggataaacta ctggaggcca tgaaccggct
     8341 ggctaaatta cccctgatgc atcagccagg ggaaaagtgg acctatggat tgaatactga
     8401 cctgctgggt tgtttggcag aagtaatttc aggaatgagt cttgatgaat ttttcagaaa
     8461 aagaattttc gaaccactgg gaatgaaaga cacctatttc ctggtgccaa aagaaaaagc
     8521 aacccgttta gtgaatttat accgtgaaga ttcaaccggc aaactggtta aagcaaccgg
     8581 taacatgctg aacgggaaaa ccattacacc caactacccg ctggaggcca gcacttatta
     8641 ttcaggtggc gcaggtttat cttccactat atatgattac gcagtttttt tacaaatgtt
     8701 attgaataac gggatgtata acggcaaaag aatactaagc cgcaatgctg tccgcatgat
     8761 gacgatgaac cagataggtg acctgtcaag aggtgatgat aaatttggat taggtttcca
     8821 ggtggtaact gaaagaggca gtgccaggac acctgcccag gccggtactt ttagctgggg
     8881 tggggctttt gccacttctt attgggtaga tccaaaagaa aagatggtga tgctattcta
     8941 ccgccagttg caaaacacaa caaagggaga agtggtggaa aaattcaggg cactgactta
     9001 tgcggcgatc aataattaat gtaactgaaa aatagtaatt ttttttatac aataacaagg
     9061 agacaataaa tcattgcttt attgcgccgg tatgcactac cggaactttc cagaaatggt
     9121 atagcaaact ttctcgttaa tgcgcctgaa aacttcgctg ttaattttcc ttaactgctg
     9181 cagcttcttt ttatcgagca gacgcccctg ctcatcataa tattgtggct gggcataaaa
     9241 attgaaaaga taatatttat tcttctgcag gactggaaac aagctgaact gcttaacagg
     9301 taccgacata aatactaaat actgtttttt cccatccaca taatagtcaa ggttgtttat
     9361 ccatgagcag ccaattcgcc gcatatcgcc tatcattttt tgtaccatag ctatatcaaa
     9421 cccaaaccgg gccagcgaat cgtttatcct gctttcatga taagcaaaat catagatata
     9481 tcttaccgaa tcggttttaa actccagtga aatatcggaa aagcctaaat cgtaaaacgc
     9541 tactgacaat aaccgggagg cagtaatttt tttatacaag gtttcaattt catgtatagt
     9601 agctttatgc tgttcataat attgtacggg atatagcttt gatgtgcagg aatacattcc
     9661 catagctgcc acaacaaaaa atagtaaagc aaaaacctta ttcattattt tcttccttat
     9721 aattaaccca gctaactccg taaataattt gctttagtga atgaagggta acctctttca
     9781 gtgttttata acccccatcc cagtatttta ttacaatgcg gtcattgttt ttttcaatac
     9841 ctgtaagcgc cacgtagtgt gttggtatac gtttttttat cctgttatgg tttttctttc
     9901 gcagtatggc attattaaga tacaggtata cttctccggc gttcaacttt tgttctaaaa
     9961 aatccggtaa attattaatt tttggtttta atagatctga gcccttcgac tgtactttat
    10021 atttacacat tttgcgcagc atacgattaa attttgccag gttagttgct gcccacattg
    10081 tattttcatc gcctttgtca tatttcctgt taaaaaaatt aaggtaccct ttaaacctgt
    10141 ttgcaaggca caaaaaccac atttggtcag caggttgaat atcgaggtca ccctggaaca
    10201 tcataagccc ggctgcatct ttaatagttt gggagggtct taatgttgcc agcctgtaat
    10261 aggactcgcc atttttataa agattaagca tggatatagt atatcggaat ggctcgtttt
    10321 tcaggcaggt gtatgttatt gcaccgaaag cacaaaaatt agtactgcgg ccattattta
    10381 tttttaacgg atactcaata ttacgtttaa gattatctaa aaatgcagaa ggtgtaatat
    10441 gaggccagaa actgctttgt tcaagggtat ttctttcacc agccaccagc tccagtgccc
    10501 gtatccttcc ggcttcattg atttcttttt gcggaacccg ttttttttgt gcacctgcgg
    10561 caagagaaat ggttacaaac agtattaata gtcccttttt catttatgaa tgtaaggttt
    10621 tgtatgcgga aggtacatac gaaagtttta atttatcgag tattgaatcc ggcttacgga
    10681 aaagacgtat tagtatgaaa caattatttg tacagcctca tccggactta cctttgctaa
    10741 aataatttgt tagggttttg tattaaaagg aacaggagtg tctgatgaag cgaaacgtat
    10801 taccggtgca tccggcaagt caaaacattg ttcgcctgtt gctaagttgt ttctttttaa
    10861 ccgccagtta caaaaaactg atcatggtgc tatggtggaa tattacaggc cattgacata
    10921 ccaggcaatc aatgattgat accgttctat aaccgatcat gaaaattctg tggacatacc
    10981 tgaaaccttt tcaatggtgg ataatacttg ccctcaccct tgcaggtatt gcacaaatac
    11041 tctctctcta tgatcctgtt atattcggaa agatcattga caattatgca ttaaatcccg
    11101 gtaacaaaac ggaagatgaa ttagttaaag gagtttcctt ctggcttggt atagcaattg
    11161 ccattgcttt ggcagccagg ttagtgatgg tcttcaaaga ttatgtgata cggctgatcg
    11221 ttcaacgttt tggtatgcag atattcaatg atggcttgcg gcaaacatta cgattaccct
    11281 accaggattt tgaggaccag agtagtggtg aaattctttc tatactccag aaagtaagaa
    11341 ccgatacaga acggtttatt acgaacttca tcaatattct gttctcttct ttcgttggca
    11401 ttggttttct tatctggtat gctgtaacca aacactgggc attgatccct gtatttttaa
    11461 ttggcgttgt actactcggc ggattaacca gcattctcag ccgttctata aaagtattac
    11521 aacgttcact cgtccggcaa aacaggcaga tgagtggtac gataacagaa tcattacgca
    11581 atattgaact ggtaaaaagc ctggggctta cttatcctga aatcagaagg ctaaagcaac
    11641 ataccaggga tatttatgac cttgaaatga agaaagtgaa aaaagtacgg acacttactt
    11701 ttttccaggg agctatccta tcttttctta aacagtccat actttttatt ttactatggc
    11761 tcattttccg aaaagtatta tcaccgggtg aattaatttc catgcaattt atttctttgg
    11821 gaatcattgg cccgttgcag gaccttggta gtattattct ctcgtatcgt gaagcagaag
    11881 cttccttaca attatttaat gagttgatgc aaaaagaacc agagtatcgc cccgaagaac
    11941 caatagatgt aggagagatt gaacatct
//
LOCUS       DAORTI010000111        11489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5790_length_11489_cov_6.350271, whole genome shotgun sequence.
ACCESSION   DAORTI010000111 DAORTI010000000
VERSION     DAORTI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5790_length_11489_cov_6.350271"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1505)
                     /gene="rseP"
                     /locus_tag="PLO70_12700"
     CDS             complement(129..1505)
                     /gene="rseP"
                     /locus_tag="PLO70_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQZ75371.1"
                     /translation="MNIIAINWANVGLQAGQLLLALSILVILHEFGHYITAKWFKCRV
                     EKFFLFFDPWFALFKKKVGDTVYGIGWLPLGGYVKISGMIDESMDKEAMKQPPQEWEF
                     RSKPAWQRLIVMLGGVIMNILVAFVIYAFILMIWGDKKIPTASMKYGVHVVDSTMYKM
                     GIRNGDKILEIDGKEVADYEKLRRNLLLGSSVTVDRNGQKVSLTLDEDLIGQLVENRN
                     KEVSGFVEVRKPAIVFFVPDTAKAYKAGLRNNDKLVAIDSVRFEFYDELQNQLNQKKN
                     KTIELTVVRDGQEVNFPVAVSDEGKIGFAPYGYNYQQMDSLGWVKLNVTKYSFFAAFP
                     AGVKKAGNELMFYIDQFKKILNPKTGAYKGVGGFKAMGSIFPKYGWDWEQFWRITAFL
                     SIVLAFMNLLPIPALDGGHVMFTLYEMITRRKPNEKFLEYAQVVGMVLLLGLMLYANG
                     NDWFGWGK"
     gene            complement(1577..2746)
                     /locus_tag="PLO70_12705"
     CDS             complement(1577..2746)
                     /locus_tag="PLO70_12705"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQZ75372.1"
                     /translation="MSAEQPIKRIAIFGSTGSIGTQALDVIAANKDKFSAEVLTAQSN
                     DDLLIRQALQFNPNVVVIGDEKKYTKVKEALSSTGIKVFTGEKALEEVAAMDCYDLML
                     AAIVGYAGLKPTLNAINNGKAIALANKETLVVAGDIIMRKAVENRVPVIPVDSEHSAI
                     FQCLVGETRNRIEKVILTASGGPFIGRKPNYLVNVKREHALQHPNWSMGAKITIDSAT
                     LMNKGLEMIEAKWLFNLKPEQIQVVIHPQSIIHSMVQFEDGSIKAQMGLPDMKLPIQY
                     AMAFPKRLPNNFPRYDFKKVSTLTFEEPDVKTFRNLGLAQEALDRGGNLPCIMNAANE
                     IAVFAFLRNRINFLDMTDVIEKTMQQVPFIENPTLEEYFESDGEARNFAADIIKL"
     gene            complement(2743..4254)
                     /locus_tag="PLO70_12710"
     CDS             complement(2743..4254)
                     /locus_tag="PLO70_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQZ75373.1"
                     /translation="MKIKSFLAKPFASYIYKGIRKGMATAVADQENILKFLLKVGRNT
                     DFGKEAGFDKVTDYEGFKQAVPIRDYEQLKPWIEKIKEGRHNVLWKGKPIYFAKTSGT
                     TSGTKYIPITKDSIPNHINTARNALLCYMAETGKTKFADGKLIFLSGSPELERVGGIP
                     TGRLSGIVNHHVPKYLRTNQMPSYETNCIEDWETKLNKIVDETINQNMTLISGIPPWM
                     QMYFDELVKRSGKKVGELFPNFNVMVQGGVNFEPYKAKLFESIGKKIDTIELFPASEG
                     FFAFQDSQEAEGLLLNTNSGIFFEFVPAGEIFNESPTRLSLKDVKVGENYALIINSNA
                     GLWGYNLGDTVKFVSTDPYRLVVTGRTKHFISAFGEHVIGEEVEYSMLKVAEESNVQV
                     TEFTVAPFVSSDEGKSYHEWFVEFEKQPANMVEFAAKLDQVLRQKNVYYDDLISGNIL
                     RPLKITPVRKNGFIDYMKSIGKLGGQNKVPRLSNDRKIADELIKWTKSPETVK"
     gene            4401..4700
                     /gene="rplU"
                     /locus_tag="PLO70_12715"
     CDS             4401..4700
                     /gene="rplU"
                     /locus_tag="PLO70_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQZ75374.1"
                     /translation="MIAVVKVAGQQFKVEKDQTLYVPRLEGNAGDKLDLEVLLADADG
                     KLSTGSSIQTKVQAEIVDQVKGDTVIAYKQKRRKGFHKKKGHRTAYTKIKVTSIA"
     gene            4745..5005
                     /gene="rpmA"
                     /locus_tag="PLO70_12720"
     CDS             4745..5005
                     /gene="rpmA"
                     /locus_tag="PLO70_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQZ75375.1"
                     /translation="MAHKKGEGSVKNGRDSQSKRLGVKIFGGQPAISGSIIVRQRGTV
                     YHPGKNVGVGKDFTLFALSDGVVEFRKGRNNKTIVSVALAEA"
     gene            5307..6983
                     /locus_tag="PLO70_12725"
     CDS             5307..6983
                     /locus_tag="PLO70_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQZ75376.1"
                     /translation="MDNAAIADNFALLAKLMDIHGENPFKSKTYAVAAFNIEKLPVSL
                     QETPREKLFSIKGIGESVGRKVIEMLDTGRLEILDEIISKTPTGVIEMLNIKGLGPKK
                     INTIWKEMGIESIGELLYACNENRLTLFKGFGEKTQKNVQESIEFYFQHQGHFLYAQL
                     DEVFPQIDNYLKKIFSPEKVHVTGRYRRQELTIDELEFVILEQNETIKPKFQTAQPPE
                     LVEETASSLLFKLRNGLKLRLYTGGNSMPEELFKSTGSPEFLEAFRKAYPSLKYSSEE
                     NGNDEAVFRAVALPYILPCLRESAIIIEKAKQDKLPELIQVADIRSIIHSHSNWSDGS
                     NTIEEMATESIKRGYEYLVISDHSQTAFYANGLKEDRIREQQRYIDELNTKLAPFRIF
                     KSIESDILNDGSLDYPDHILKTFDLVIASVHSNLGMPEEKANARLIKAIENPYTTILG
                     HMTGRLLLSRSGYPVDHKKIIDACVNNQVVIELNAHPRRLDIDWKWIEYALEKGVLLS
                     IDPDAHALDGFDDVKYGVLAAQKGGLTKQKNLSSFSLREFEEFLRHQKEK"
     gene            7050..7433
                     /locus_tag="PLO70_12730"
     CDS             7050..7433
                     /locus_tag="PLO70_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75377.1"
                     /translation="MNKKSTTGLSIFLKELLETLNDSTIAYRQYIEGGKTFQFAQQLK
                     LYNGKAFDLLTSNKQLLPENLQQDADALIQHYRVWTEKWEALANELKPVPGDLFVFAN
                     TITFPRQAAHNLETTYETLKKENKQ"
     gene            complement(7437..7883)
                     /locus_tag="PLO70_12735"
     CDS             complement(7437..7883)
                     /locus_tag="PLO70_12735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HQZ75378.1"
                     /translation="MAFLEKKVAVKKSILPGAGKGLFAKEMIPKGTRIVEYKGEITTW
                     KEVDHNEGSNGYIYYVKRNHVIDASRTTEALARYANDARGLARVKGITNNAEYTEEGL
                     KVYIESTKDIPAGTEILVDYGKEYWDVIRYNRKLESQRKRTSSPQD"
     gene            8004..8456
                     /locus_tag="PLO70_12740"
     CDS             8004..8456
                     /locus_tag="PLO70_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75379.1"
                     /translation="MKKVFLLILFLAAAQLVLTAQEYINKKKGEVITMLKIKYQQTPL
                     SVLTDTVKMKLENTFAMPFDYIFTFDKTGRCKSETIITRCDSCLTGLLQKVLDLKKFE
                     WNKINENQYISKFEEKLMIELPAENYDHSFSILKVEWTKELYKLLLKQ"
     gene            complement(8462..8812)
                     /locus_tag="PLO70_12745"
     CDS             complement(8462..8812)
                     /locus_tag="PLO70_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75380.1"
                     /translation="MKKYLILFFLVALTVVLSNCGSSRKAAATVPKLNYETNLQATIL
                     SNCSPCHIPAKGGNKKPYDNYTNVKTDIDEIVRRIEMNPGEKGFMPFRKTTRLSDSTI
                     AIFKQWRTDGLLEK"
     gene            8940..10142
                     /locus_tag="PLO70_12750"
     CDS             8940..10142
                     /locus_tag="PLO70_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ75381.1"
                     /translation="MLAATARTYRNAYSGLSKETWLLSLIMLINRSGTMVVPFMTLYL
                     TSPEMGYSVGQAGIVFGLFGAGAFSGAWLGGRLTDKIGFYPVQLFTLFAGGILFMVLG
                     QMKTYPLICTFTFLLSFVNEAFRPANSTAIAFYSKTENRTRSYALNRLAINIGWAVGS
                     GLGGFLADMSYELLFWVDGITNISAAILMWVVLKPVDYKPEQHKDIGHPGTSLSAYRD
                     KKYLWFILFTMLFASCFFQLFTNLPVYLKNELHFSKPFIGILMAFNGILIAIVEMVLI
                     YRLEGKRNILHYITIGVFLVGLAFLILNIPGIGPLLAFAMITTVTFGEILSMPFMNSY
                     WITRTHPSNRGQYAALYTMAWSAAQTLGPMGGAQLVEYSGFNLLWWSVGGLCIITSFS
                     FLKLRNSS"
     gene            10204..10638
                     /locus_tag="PLO70_12755"
     CDS             10204..10638
                     /locus_tag="PLO70_12755"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75382.1"
                     /translation="MKKIYCAGIILLTSIMSCKQQDVLTREEVMAVINRFDEGWKNKD
                     ARVVDSVLSQQYIYFTQSGYTFDRASLIQTADSSIYLLQTMERQEFTIQLEGNTAVVN
                     TVWKGKGTYHGQEFDDNQRCSITIVKNDGQVKILSEHCTPIN"
     gene            complement(10640..11329)
                     /locus_tag="PLO70_12760"
     CDS             complement(10640..11329)
                     /locus_tag="PLO70_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQZ75383.1"
                     /translation="MKVIGLVGGTSWISSVDYYRLFNEITNQRLGGNEAAKIVLYSVN
                     YGEIVTYTHQGNWDAIAAIIADAAQKVEKAGADCILLGANTMHHVAERVQQSVKIPLL
                     HIADVVGKAITEKQLKKVALLGTKYTMLFDFYTRKLADYGIETIIPDAEGVDFVNSAI
                     YNELGKGKFLPETKQGFLHLIGKLAGQGAEGVILGCTEIPMLIKQEDTLVPVFDTTLL
                     HATAAVDFALQ"
BASE COUNT         3424 a         2514 c         2118 g         3433 t
ORIGIN      
        1 agttttcgta ttcagattaa actgctaatt acgcaacgca gtacatgctt acactctcaa
       61 agatctacgc tgtcaaaacc aaacgccccc ataatggtaa attccaaaac aaaaaattca
      121 atatccaatt atttccccca tccaaaccag tcattcccat ttgcatacaa catcaatccc
      181 aataataata ccattcccac cacttgtgca tactccagga atttttcatt gggttttctg
      241 cgggttatca tttcatataa tgtaaacatt acatgccccc catccaaggc aggaataggc
      301 aataaattca taaaagcaag tacaattgat aagaacgctg taatcctcca aaattgttcc
      361 cagtcccaac cgtattttgg gaaaatagag cccattgcct taaatccgcc aactccttta
      421 taggcaccgg ttttagggtt taatattttc ttgaactgat caatataaaa catcagttca
      481 ttgcccgcct ttttaacacc ggccggaaaa gcagcaaaga aactatactt cgtcacattc
      541 agtttcaccc atccaaggct gtccatctgc tggtagttat acccgtatgg agcaaatcca
      601 atctttcctt catcacttac tgccaccggg aaattaactt cttgcccatc ccttactact
      661 gtaagttcaa tcgttttatt tttcttctgg tttaactgat tttgtaattc atcgtaaaac
      721 tcgaaccgga cagaatcaat ggccaccagt ttatcattgt ttcgtaagcc ggctttatat
      781 gctttggcag tatcaggtac aaaaaataca atggctggtt ttcttacttc cacaaaacca
      841 ctaacttctt tattcctgtt ctctactaat tgcccgatca ggtcctcatc taatgtaagg
      901 ctaactttct ggccgtttct gtcaacagta actgaacttc ctaaaagtaa gttgcgcctc
      961 agtttttcat aatctgctac ttccttacca tcaatttcca atatcttatc cccattacga
     1021 atacccattt tgtacatggt actgtctacc acatgtaccc catacttcat cgatgcagtg
     1081 ggaatctttt tatctcccca gatcatcagg ataaaagcat aaataacaaa cgccaccagg
     1141 atgttcatga tcacccctcc cagcattaca attaaacgtt gccaggccgg cttacttctg
     1201 aattcccatt cctgcggagg ctgcttcatt gcttccttat ccatactttc atctatcatt
     1261 ccggatattt ttacatatcc tcccaacgga agccacccga ttccatatac agtatcacct
     1321 actttctttt taaacaatgc aaaccagggg tcgaagaaaa ggaaaaattt ctctacccgg
     1381 catttgaacc atttggctgt aatataatgt ccaaactcat gtagtatcac caatatagat
     1441 aaggcaagca gtaattgtcc tgcctgtaaa cccacatttg cccagttaat cgctattata
     1501 ttcataataa tatttcaaaa taaaatcccg ataattatcg ggaccaaatc cacaaagcag
     1561 atttagaatt acagtattac agttttatta tatccgctgc aaagttacgg gcctcgccat
     1621 cgctttcaaa atattcctcc agtgtgggat tctcaataaa tggtacctgt tgcatagttt
     1681 tttctataac atccgtcata tcaagaaagt taattcggtt gcgcagaaag gcaaaaacag
     1741 caatttcatt tgccgcattc ataatacatg gcaggttacc gcccctgtcc aaagcctcct
     1801 gggctaatcc taaatttctg aatgttttaa catccggctc ttcaaaagtg agggtactga
     1861 ccttcttaaa atcatagcgg gggaaattat tgggcaatcg tttaggaaat gccatcgcat
     1921 actgtatagg cagtttcata tccggtaatc ccatctgtgc ttttatgcta ccatcttcaa
     1981 actgcaccat gctgtgaatg atgctttgcg gatgaataac cacctgtatt tgttccggct
     2041 tcaggttgaa taaccatttt gcttcaatca tctccagtcc tttattcatc agtgtggcag
     2101 aatcgatcgt tattttggca cccatactcc agttaggatg ttgcagggca tgttctcttt
     2161 ttacattgac aaggtaattc ggtttacggc caataaaagg tccgccggag gcagttaaaa
     2221 ttactttctc aatcctgttt ctcgtttctc ccaccaggca ttgaaaaata gctgagtgtt
     2281 ctgaatccac aggaattacc ggcacacggt tttcaacagc tttgcgcatg atgatatcac
     2341 cggccactac caatgtttcc ttattagcca atgcaatggc tttaccatta ttgatagcat
     2401 ttaatgttgg cttcaatcct gcataaccaa ctatggctgc cagcatcagg tcataacaat
     2461 ccatagctgc aacttcctct aatgcttttt ccccggtaaa tacttttata cctgttgagg
     2521 acagggcttc ttttactttg gtatattttt tttcatcacc gataactact acgttgggat
     2581 tgaattgcaa tgcctgcctt attagcaaat cgtcattact ttgtgccgtc agcacttcag
     2641 cagaaaactt atccttattg gcggcgatca catccaatgc ctgcgtaccg atggaaccgg
     2701 tggaaccgaa aatggcaatt ctttttatag gttgttctgc actcatttaa ctgtttcagg
     2761 acttttcgtc catttaatta attcatccgc aatttttcgg tcattactca gtctcggcac
     2821 tttattctgc cctcctaatt taccaataga tttcatgtaa tcaataaatc cgtttttacg
     2881 aacaggagtg atcttcagcg gcctgagtat attaccactg atcaaatcat cataatacac
     2941 attcttctga cgaagcacct ggtctagttt ggcggcaaac tctaccatat tggccggttg
     3001 cttttcaaac tcaacaaacc attcatggta tgacttacct tcatcactgc tcacaaacgg
     3061 cgcaactgta aattcagtta cctgtacatt tgactcctct gccactttta acatgctata
     3121 ttccacttct tcaccaatca catgttctcc aaatgctgaa atgaaatgct tggttcttcc
     3181 tgttaccact agccggtaag ggtctgtcga gacaaacttc accgtgtcac caagattata
     3241 accccagagc cctgcattgc tatttattat cagtgcatag ttttctccca cttttacatc
     3301 ctttaatgat aatctcgtgg gactttcatt aaaaatttca cccgcaggca caaactcaaa
     3361 aaagatgccg ctattggtat ttaataataa tccttccgct tcctgcgaat cctggaacgc
     3421 aaaaaaacct tcactcgccg ggaacagttc aatcgtatca attttcttac ctatactctc
     3481 aaacaatttt gctttataag gttcgaagtt cacaccgccc tgtaccatca cattgaaatt
     3541 ggggaacaac tctcctactt tcttaccgct cctttttact agttcatcaa aatacatctg
     3601 catccagggt ggtatgccgc tgatgagtgt catattctga ttaatagttt cgtctactat
     3661 cttattcagt ttcgtttccc aatcttcgat acaatttgtt tcatacgatg gcatctggtt
     3721 tgtacgaagg tattttggca catggtgatt cactatccca ctaagcctcc cggtagggat
     3781 tccgcctacc ctttccagtt ccggtgagcc tgaaaggaag atcagttttc catcagcaaa
     3841 tttggtcttg cccgtttcgg ccatataaca aagcagggca ttccgggcag tattaatatg
     3901 attagggata gaatccttgg taatgggaat atatttggta ccactggttg tacctgaggt
     3961 tttggcaaaa tagataggtt tccctttcca gagtacatta tgccttccct cctttatttt
     4021 ttcgatccag ggctttagtt gctcgtagtc ccggatgggt acagcttgct taaatccttc
     4081 gtaatcagtt accttatcaa atcctgcttc tttcccaaaa tcggtgtttc gacccacttt
     4141 caggagaaat tttaaaatat tctcctggtc ggccacggca gtagccatac ctttgcggat
     4201 acctttatat atataagaag cgaacggttt agctaagaaa gacttgattt tcatactcaa
     4261 agtaaaaagg aaaccccttg ggggctacaa atataacgga tggccgggca aaaccgggtc
     4321 atttaatgtt cataaatctt tgattccccg tttgccactt caatttttcc ccttaccttt
     4381 gccgtcctaa ttttaagagt atgatagcag tagtaaaagt agccggacag caattcaaag
     4441 tggaaaaaga tcagactttg tacgttcccc gtttagaagg aaatgccggt gataaactgg
     4501 atcttgaagt attgctggca gatgctgatg gcaaactttc aacaggttct tctatacaaa
     4561 caaaagtaca ggctgagatc gttgaccagg taaaaggtga taccgtgatt gcctacaaac
     4621 aaaaaagaag aaaaggtttt cacaaaaaga aaggtcatcg tactgcgtat acaaaaatca
     4681 aagtaaccag cattgcataa ggcaatcaac ctaactcaat cattaatttt aaaaacatcc
     4741 tgatatggca cataagaaag gtgaaggtag cgtaaagaac ggccgtgatt cacaaagcaa
     4801 acgccttggt gttaaaatct ttggtggtca acctgctatt tccggtagca ttatcgttcg
     4861 ccagcgtggt acagtttatc atcctggaaa gaatgttggt gttggaaaag actttacatt
     4921 gtttgcatta agtgatggtg tagttgaatt ccgcaaagga agaaacaaca aaacaattgt
     4981 ttctgtagca cttgctgaag cctgagccgt tttccctgtt ttgaattttg tggaaaaaaa
     5041 atttgggtag tataaaataa ttgttatttt tactatcgtt aacccattta ttactaaaca
     5101 ttaaattcaa acaaaatggc tacaaagaaa aaagctgcta aaaaagctgc tccaaaaaag
     5161 aaagctgctc caaagaaaaa agctgctaaa aaagctgcta agaaaaagaa ataagtttga
     5221 ttgtttctta aaagaaatag taagtcctcc gaatttcggg ggactttttt tatccttctg
     5281 ctttcaataa atattaattt gcggctatgg ataatgctgc tatcgccgat aattttgccc
     5341 tgcttgccaa attaatggac atccatggcg aaaacccgtt taagtcgaaa acctatgcag
     5401 tagcggcttt caacatagag aagcttcctg tttccctgca ggaaacacca agagaaaaac
     5461 ttttcagtat taaaggtatt ggtgaaagtg tgggtcgaaa agtaatcgaa atgctcgata
     5521 ccggcaggct ggaaatactg gatgagatca tcagcaagac tccaacaggt gttattgaga
     5581 tgctgaatat taaaggtctt ggcccgaaaa aaattaatac tatctggaaa gaaatgggca
     5641 ttgaatcaat cggtgaacta ttatatgcat gcaatgaaaa ccggttaacc ctctttaaag
     5701 ggtttggaga aaagactcaa aaaaatgtcc aggagtctat tgaattctat tttcagcacc
     5761 agggacattt tttatacgct caactggatg aagtattccc gcagatagat aattacctga
     5821 aaaaaatatt ttccccggaa aaagtacatg taacaggaag atacaggaga caggaattaa
     5881 cgatcgacga actggagttt gttattcttg aacagaatga aacaataaaa ccaaaatttc
     5941 aaactgcgca accacctgaa ttagtggaag aaactgccag cagtctttta tttaagttaa
     6001 ggaatgggct gaaacttcgt ttgtacaccg gtggcaatag catgccggaa gaattattca
     6061 agagtactgg cagtcctgaa tttttagaag cattcagaaa ggcttaccca tcgttgaaat
     6121 acagcagtga agaaaatgga aatgatgaag cagttttcag ggccgttgcc cttccttata
     6181 tacttccctg cctccgggag tcagccatca tcattgaaaa agcaaaacaa gataaactcc
     6241 cggaactgat acaggttgct gatatccgtt ctatcattca cagccatagc aactggagcg
     6301 atgggagcaa tacaattgaa gagatggcca ctgagtctat caaacggggt tatgaatatc
     6361 ttgttatttc cgaccactca caaacagcct tctatgctaa tggactgaaa gaggacagga
     6421 tcagggaaca acagcggtat attgatgaac tgaatacaaa gctggcgcca ttcaggatct
     6481 ttaaaagcat cgaaagcgat atattgaacg atggcagcct ggactacccg gatcatatat
     6541 taaagacctt tgacctggta attgcctctg tacatagcaa cctgggtatg ccggaagaaa
     6601 aagccaatgc ccggctgatc aaagctattg agaacccata tactactatt ctggggcata
     6661 tgaccggacg gctgctgttg agtcgcagcg gttacccagt tgatcacaaa aaaattattg
     6721 acgcctgtgt taataaccag gtagtgatag aactcaatgc tcacccgaga agactggata
     6781 ttgactggaa atggattgag tatgcgttgg agaaaggcgt cttactttcc attgacccag
     6841 atgcccatgc cctggatgga tttgatgatg tgaagtacgg agtacttgct gcgcagaaag
     6901 gtgggttaac aaagcaaaag aacctgagta gcttttcctt gagagaattt gaagagtttt
     6961 tacggcatca gaaagaaaaa tgatgtatag aattttagtg cacatattaa gtatgcacac
     7021 tcaaaatatg ctatataaaa atctccctta tgaataagaa atctactacc ggattatcaa
     7081 tcttcctgaa agaacttctt gaaacattga atgattccac tatagcatac cggcagtata
     7141 tagaaggagg gaaaacattc caattcgccc aacaacttaa attatacaat ggcaaagcat
     7201 tcgatctgtt gacttccaat aaacagctgt tgcccgaaaa cctccaacag gatgctgatg
     7261 cactgatcca gcattaccgt gtgtggacag aaaaatggga ggccctggcc aatgaattaa
     7321 aaccagtacc gggtgaccta tttgtatttg ccaatacaat tacttttccc aggcaggcag
     7381 cacataacct ggagacaaca tacgaaacgt taaaaaagga aaataagcag tgagttttaa
     7441 tcctgcggtg agcttgttct tttccgttga gactccagct tcctgttata tcttatcaca
     7501 tcccaatact cttttccata gtccaccagt atctctgttc cggccgggat atctttagtt
     7561 gactcaatgt acaccttcag cccttcttcg gtatattctg cattattcgt aatgccttta
     7621 accctggcca gtccacgggc atcattggca taccgggcca gggcttccgt agtcctgctg
     7681 gcatcgatca catggttacg tttcacataa tagatgtagc cattcgaacc ttcattatga
     7741 tccacttcct tccaggtagt tatttctcct ttgtattcaa cgatccgggt tccttttgga
     7801 atcatttcct ttgcaaacaa acccttacca gctcccggga gaattgattt tttaactgct
     7861 actttctttt ctaaaaatgc catactaaaa tttttcttca tttttggaaa cgcaaatata
     7921 aaagatatag ccatagaaaa gctgacactt ttcgcatata caggaaagca actaccttta
     7981 ctgcatattc gtctgtaatc atcatgaaaa aagtattcct gcttattctc ttcttagccg
     8041 ccgcacaatt ggtattaacc gcccaggaat atataaataa aaagaaaggc gaggtgataa
     8101 caatgctgaa aattaaatac cagcagacac cattatctgt gttgacagac accgtaaaaa
     8161 tgaaactgga gaacacattt gcaatgccgt ttgattatat tttcactttt gacaaaacag
     8221 gcaggtgtaa atcggaaact ataataaccc gatgcgactc ctgcctcacc gggctattgc
     8281 aaaaagtact tgacctgaaa aaatttgaat ggaataaaat caatgagaac cagtacattt
     8341 caaaattcga agaaaaattg atgatcgaac taccagctga aaattatgac cactcctttt
     8401 ccattcttaa agtggagtgg accaaagaac tatataagct gttacttaaa cagtaattac
     8461 cttatttttc cagcaaccca tcagttctcc attgtttaaa tatggcaatc gtgctatcac
     8521 tgagccgggt tgttttcctg aatggcatga atcctttttc accggggttc atttcaatgc
     8581 gacggacgat ctcatcaata tcggttttta cattagtata attatcataa ggctttttat
     8641 tgccgccttt ggcaggtata tgacaggggg agcaattgga caaaatagtg gcctgcaggt
     8701 ttgtttcata attcagtttt ggaacggtgg cagcagcttt tctggaactt ccgcaatttg
     8761 ataatactac tgtcagtgca accaggaaaa aaaggataag atatttcttc atattgatta
     8821 ctttttatac ctgaatatac attgattata gaaaataggg ttgaaaaata ttctatctaa
     8881 tcgttccggt tataccaacg gttgctttct tttttattca ttccggtacc tttgcgccca
     8941 tgttagctgc cacagccaga acatatcgca atgcttattc aggtctttca aaggaaacct
     9001 ggctgctttc tcttatcatg cttatcaaca gaagcggtac gatggtggtt cctttcatga
     9061 ctttatacct aaccagcccg gagatgggtt attccgtcgg gcaggcaggt attgtattcg
     9121 gcttatttgg tgccggagca ttcagtggcg cttggttggg tggccggcta actgataaga
     9181 tcggttttta tccggtacaa ctatttacgc tgttcgccgg cggaatacta ttcatggtat
     9241 taggacaaat gaaaacctat ccgctaatct gcacctttac cttccttctc agttttgtga
     9301 atgaagcttt ccggccggcg aattccaccg caattgcatt ttacagtaaa acagaaaacc
     9361 gcacccgttc ttatgcgttg aacaggcttg caattaatat cggatgggcg gtcggtagtg
     9421 ggctgggcgg tttcctggca gatatgagct atgaactgct gttttgggtc gatggcatca
     9481 ccaatatcag tgcagcaatc ctgatgtggg tggttctaaa accggttgat tacaaaccgg
     9541 aacaacataa agatattggc catccgggta catcactatc tgcctacagg gataaaaaat
     9601 acttatggtt tatccttttc accatgcttt ttgccagttg cttttttcaa ttattcacta
     9661 acctgccggt gtacttaaaa aatgaactgc acttttcaaa gccattcatc ggtatactga
     9721 tggccttcaa cggcattctc attgccatcg tagaaatggt cctcatatac cggctggaag
     9781 gaaaaaggaa tatactccac tacataacta tcggggtatt tttggtagga cttgcgttct
     9841 taatcctgaa tattcccggc attggtccgc tacttgcttt tgctatgatc acaactgtta
     9901 cttttggtga gatactttcg atgccattca tgaacagtta ttggatcacc aggacacatc
     9961 cttctaaccg tggacaatat gcggcacttt ataccatggc ttggtcagct gcgcaaaccc
    10021 ttggccctat gggtggcgca caactggtcg agtattcagg tttcaattta ctatggtggt
    10081 cggttggcgg gctctgtatt attacgtcct tttcattcct gaaattgcgc aacagcagct
    10141 aactatccaa tcctgcggat tttgattatt tataactatt tctcatttgt atctttacca
    10201 actatgaaaa aaatttattg cgccggtatt attttactta catcaatcat gtcctgtaaa
    10261 caacaggacg tattgaccag ggaggaagta atggctgtca ttaatcgttt tgatgaaggc
    10321 tggaagaata aagatgcacg ggtagttgat tccgttttat cgcagcaata tatctacttt
    10381 acacaatcag ggtatacgtt tgacagggcc agcctcatac aaacagctga ttcatcaatt
    10441 taccttttac aaacaatgga aaggcaggaa ttcacgatac aactggaagg aaatacagcg
    10501 gtggtcaata cagtctggaa aggtaaaggc acttaccacg ggcaggagtt tgatgataac
    10561 cagcgttgca gcatcacaat tgtaaagaat gatggccagg taaaaatttt atctgaacac
    10621 tgcactccta tcaactaatt tattgtaatg caaaatccac cgcggcagtg gcatgaagca
    10681 aggtagtatc aaaaacaggt actaaagtat cttcctgttt aatgagcatt ggaatttcag
    10741 tacaacccaa aattactcct tcagcaccct ggcctgctaa tttaccgatc aggtgaagaa
    10801 acccttgctt tgtttcagga agaaatttcc ccttacctaa ttcattatag atagcactat
    10861 ttacaaaatc gacaccttcc gcatccggga taattgtttc tatcccataa tcagccagct
    10921 ttcttgtata aaaatcaaaa agcatcgtat actttgtacc tagcaaggca acttttttca
    10981 attgcttttc cgtaattgct ttcccaacaa catcagcaat atgaagcaaa ggtatcttaa
    11041 ccgactgctg aactctttct gcaacatgat gcatagtatt agccccaagc aaaatacagt
    11101 cagcccctgc cttttccact ttttgtgcag cgtctgcaat gatcgcagcg attgcatccc
    11161 agttaccctg gtgcgtatag gtcacaattt caccgtagtt tacagaatac aatacaattt
    11221 ttgcagcttc attaccaccc aggcgctggt tcgtaatttc attgaaaagc ctgtaataat
    11281 ccacagacga tatccaagat gtgccgccaa ctagaccaat aactttcatt tgttaaaaat
    11341 acaagaaagc aattgatttg aatctatttt actttcttaa agcatctttt ttagccaggt
    11401 ttgtcgtcgt aaaaaggtga taattccaaa acctctaaaa ccctacattt gcctaaatta
    11461 aaaattgaat gaaaaaaatt ttcacaata
//
LOCUS       DAORTI010000112        11466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5812_length_11466_cov_11.079748, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000112 DAORTI010000000
VERSION     DAORTI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_11466_cov_11.079748"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLO70_12765"
     CDS             <1..259
                     /locus_tag="PLO70_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HQZ75384.1"
                     /translation="EDHHHAALIADAISKKNFIKMMLPVETNIIIFELTNTISAPQLV
                     AKLKEYDILGYAISPVRVRLVTHLDISKEMIQKTIEVFNQL"
     gene            272..1924
                     /gene="pgi"
                     /locus_tag="PLO70_12770"
     CDS             272..1924
                     /gene="pgi"
                     /locus_tag="PLO70_12770"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQZ75385.1"
                     /translation="MLPKINPTTTNAWHLLQQHFTEIKTVQLKELFKTDPDRFKKYSL
                     CTDELVFDYSKNIITGKTKQLLLQLTEECKVKEAINAMFNGEAINETEKRAVLHVALR
                     NFSNRPVYAQGNDVMPEVKKVLRKMKAFCTAVHSGKHRGYTNKKIKYIVNIGIGGSDL
                     GPLMITEALKPYWVDDIQTYFVSNVDGTHIAETLKKVNPERTLFLIASKTFTTQETMT
                     NAHTAREWFLKKAKNEKHIAKHFVALSTNEKEVVKFGIDSKNMFEFWDWVGGRYSLWS
                     AIGLSIALTIGYKNFEQLLKGAHKIDNHLIEAPFEKNIPVLMALIGLWYTNFFGSQSE
                     AILPYDQYLHRFAAYFQQGNMESNGKSVDRNGEPVEYATGPVIWGEPGTNGQHAFYQL
                     IHQGTPLIPCDFIALAQSHNPIGDHHIKLLSNFFAQTEALMNGKTEDEAEKELEKQGL
                     NAEDIVKLLPYKIFKGNRPTNSFLIKKLTPYTVGQLVALYEHKIFVQGVIWNIYSFDQ
                     WGVELGKQLANKILPELQTNVPVIDHDSSTNGLINAWKQMKK"
     gene            1953..2351
                     /locus_tag="PLO70_12775"
     CDS             1953..2351
                     /locus_tag="PLO70_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75386.1"
                     /translation="MKTIFLLFGLFGCYGVSAQQKDLFDIEKHLHKKSIQKKGTKHRL
                     LQRDNFNNYKSVPEKNPANKSYTGVLSYTLPNGDKLYSLPTDNMPCVVPDMNQYNIMP
                     NAGTSTIVILQNRPEIIPNPAKPFKIPATK"
     gene            complement(2673..6038)
                     /locus_tag="PLO70_12780"
     CDS             complement(2673..6038)
                     /locus_tag="PLO70_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQZ75387.1"
                     /translation="MQSKFTLNQRRRNLFGLSPLFLILFFLVAGKINSQQYVNGNLST
                     GATSSNGVAAPAGFTWSEVQAGNGTAGFGNNITDFMLADDFTIAAGSWNLTKITFYAY
                     STGFAGPTSPYTDVRVAIYNTNPSVGNPAPIYGDLTTNRFLASSTASMYRIFNATPGT
                     TRQIWKIEATVATVLNAGTYWIQWGVGNGGLSNFSPASTVVGTTTQPGNNALQHALAG
                     NTWANANDGTNPQDFPFIIDYTTGACTGTPAPGNTLSTVSTVCPGINFTLSLQNGTAG
                     SGVTYQWQSSPDGTTWTNIAGATNSSYTTTLTATTQYRCNVTCSGNTGTSTPVTVALT
                     PPSNCYCTPPASDCTDDDVITRVRISTLDNSSGCSAGPPTGYSNYTTTVAAPSVFSGA
                     ANPITVNAPATWTEGICVWIDYNQNGQFETTEYTSLGNKPAGQTSVTGSIAIPSTALN
                     GTTRMRVRIRFGGVFTNAQACTGSTFSETEDYSVTITPCIPVTITSAPSSTSVTCGNN
                     VSFTVAASGSLPSYSWQYRTSSSGVWQTVTNGGIYSGATTATLSLANVSADYNGYQYR
                     ALVTGGCSAVDFTSPPATLTVNAIVPVVNPASATICEGTIQALTLTNSVSAATTVTFN
                     ASAGLPLVVTDNDPTGVTNSVAVSGIPAGSVITNVGIRFNMAHTWVGDLVMNLKAPNG
                     QTLNLIGLLDGGTGSNGTDDFVNTVVDSISTTAMSGAPAPRTGSFRADRFTATVPTVG
                     PTTTNFWSPLLGTMNGDWTLAMCDLGGGDVGTLTSWSISITYVAPVFAQGVWAGPAGT
                     IFTDAAATIAYTGTPATMVYVKPTANTNNYTVSFTTPTPCTSATTTVPVNVSHPATGL
                     AVTPSTRAVCLGGSTTFVASTTGGNPIAYQWQLSTDGGLTYNNISGATSSTLTVSSVT
                     QSMSGNRYRFVVTSAPCTPAVTSTAFGTLTVNPLPTVTLSSPVVNLVPGRTTTITGTS
                     TPAAAAGGWSWTLNGSGLSGTTNSQVVNIDGVGSYQATVTDVNGCVSSSNTLVIGSEA
                     SDKLWIYPNPTTGAFQVRLYYEGGLAEKRVVSIYNPLGQVITSREFTLVSGTAPYMRM
                     DFDLGGVKAKGTYVVKVANMFTGKVVSGLVLVQ"
     gene            complement(6218..9496)
                     /locus_tag="PLO70_12785"
     CDS             complement(6218..9496)
                     /locus_tag="PLO70_12785"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQZ75388.1"
                     /translation="MQSKFTLKKKMFLLTRIFAMALIFLSLSKTSTAQVLYSEGFTDI
                     TVLPGWANQNLSSPVGSTGWFQGNSGVFPSQAGAATAYIGANFNNTTGANTISNWLFM
                     PNISIKNGDQFTFYTRVPTGGATFPDRLQVRMSLNGASTNAGATATSVGDFTNLLLDI
                     NPTYGAAYPETWQQFTVIISGVPAPTSGRLAFRYFVENGGPSGANSNYIGIDEVVYTA
                     IGACSGTPAPGATNSTANPVCPGINFTLSLATPGSGVTYQWQSSPDGTTWTNITGATN
                     GTLTTSQTVATYYRCNVTCGANTGTSTALQVNMAAPSACYCTPAATTCTLDDEILNVR
                     LGTLNNPSSGCSGAGYTYYSAVAAPDVFIGAANPMAVSVGPGGTEHVAVWIDYNQNGQ
                     FEATEFTALGSGNGSTINGIVAVPASALTGTTRMRVRVRWSTALTGTQACTGGFFGET
                     EDYNVNLVTCIPVTITSAPSSTSVTCGNNVSFTVAASGSLPSYSWQYRTSSSGVWQTV
                     TNGGIYSGATTATLSLANVSADYNGYQYRALVTGGCSAVDFTSPPATLTVNAIVPVVN
                     PASATICEGTIQALTLTNSVSAATTVTFNASAGLPLVVTDNDPTGVTNSVAVSGIPAG
                     SVITNVGIRFNMAHTWVGDLVMNLKAPNGQTLNLIGLLDGGTGSNGTDDFVNTVVDSI
                     STTAMSGAPAPRTGSFRADRFTATVPTVGPTTTNFWSPLLGTMNGDWTLAMCDLGGGD
                     VGTLTSWSISITYVAPVFAQGVWAGPAGTIFTDAAATIAYTGTPATMVYVKPTANTNN
                     YTVSFTTPTPCTSATTTVPVNVSHPATGLAVTPSTRAVCLGGSTTFVASTTGGNPIAY
                     QWQLSTDGGLTYNNISGATSSTLTVSSVTQSMSGNRYRFVVTSAPCTPAVTSTAFGTL
                     TVNPLPTVTLSSPVVNLVPGRTTTITGTSTPAAAAGGWSWTLNGSGLSGTTNSQVVNI
                     DGVGSYQATVTDVNGCVSSSNTLVIGSEASDKLWIYPNPTTGAFQVRLYYEGGLAEKR
                     VVSIYNPLGQVITSREFTLVSGTAPYMRMDFDLGGVKAKGTYVVKVANMFTGKVVSGL
                     VLVQ"
     gene            complement(9688..10128)
                     /locus_tag="PLO70_12790"
     CDS             complement(9688..10128)
                     /locus_tag="PLO70_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HQZ75389.1"
                     /translation="MAYENNDKKMESIYSKRIRAGKRRTYFFDVRATRGNDYYLTITE
                     SRKRFDDNGYDRHKVFLYKEDFNKFIKALGEAVDYVKTELMPDFDFDAFNHDENPDEY
                     NQNGAEVSQEAAPVVESAGDVVSETPVAETNTSPDTSGEEVDKW"
     gene            complement(10291..>11466)
                     /locus_tag="PLO70_12795"
     CDS             complement(10291..>11466)
                     /locus_tag="PLO70_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ75390.1"
                     /translation="SLSDLTTNAQETYSGIRVIKSFVQEKAMLGFFTRTAEDFKKNVL
                     GLVKVEAIYFPSITLLIGLSTLLTIMIGGLYYIYGGHNIGINTIVEFVMYINMLTFPV
                     SAIGLTASMIQRAAASQKRINEFLDIKPGIRNQPAALNIPLQGDIKFDKVSFTYPHTG
                     IEAIKDFSLHIRSGEKVAIVGRTGSGKSTLAQLLLRMYDVTDGTLEMDGNNISKIDLK
                     VLRQQISYVPQDVFLFSETVENNIGFGMMQFDRNAVENAAIKASVEKEIANFSGQYDT
                     MIGERGVTLSGGQKQRITIARALIKDSNIVVFDDCLSAVDARTEKEIISNLNDYLHQK
                     TAIIITHRIFSLLNFDKIVVLEAGRIIEQGTHDELIQQKGYYAKLYLRQQQQDRGTN"
BASE COUNT         3291 a         2625 c         2336 g         3214 t
ORIGIN      
        1 agaggatcat catcatgctg ccctgatcgc tgatgcaatt tcaaaaaaga attttataaa
       61 aatgatgttg ccggttgaga ccaatattat catatttgaa cttactaaca ccatctctgc
      121 acctcaactg gtggcaaaac ttaaggaata tgatatttta ggttacgcaa tttcacctgt
      181 acgggtaaga ctggttacac acctggatat cagcaaagaa atgatacaaa aaaccattga
      241 ggtctttaat caactatagc attttcaact tatgttaccc aaaatcaatc ccactactac
      301 caacgcctgg catcttctcc aacagcattt tacagaaata aaaactgtcc agctgaaaga
      361 gctgtttaaa accgatccag accgtttcaa aaaatattca ttgtgtacag acgaactggt
      421 atttgactac tcaaaaaata taattaccgg caaaacaaaa caattactgc tgcagttaac
      481 tgaagagtgc aaagtgaaag aagcgatcaa tgctatgttt aatggcgagg cgatcaacga
      541 aacggaaaaa agagcagtac tgcatgtagc attacgtaat ttttccaatc gccctgtata
      601 tgcacaaggc aatgatgtga tgccggaggt aaaaaaagta ctccgtaaaa tgaaagcttt
      661 ttgcactgcg gtacatagtg gtaagcacag gggttacacc aataaaaaga taaaatacat
      721 cgtcaatatt ggtattggcg gaagtgacct cggcccatta atgataaccg aagctttgaa
      781 accatattgg gtggatgata tccaaaccta ttttgtttct aatgtcgatg gtacacatat
      841 tgccgaaacg ctaaaaaaag ttaatccgga aagaacatta ttcctcattg cgtccaaaac
      901 attcactacc caggaaacaa tgaccaatgc tcatacagca agggagtggt tcctgaaaaa
      961 agcaaagaac gaaaaacaca ttgccaaaca ttttgttgcg ctgagtacca atgaaaaaga
     1021 agttgtcaag tttgggattg acagtaaaaa catgtttgaa ttctgggatt gggtaggcgg
     1081 ccgctattct ctctggagtg ctatcggttt atccatagcc ctgaccattg gttataaaaa
     1141 ttttgaacaa ctgttaaaag gtgcccataa aattgataac catttgatag aagctccatt
     1201 tgaaaagaac atcccggtat tgatggcctt aattggttta tggtatacca atttctttgg
     1261 ttcacaaagc gaggctattc ttccatatga ccaatacctg catcggtttg ccgcttattt
     1321 tcagcagggc aatatggaaa gcaacggtaa aagtgttgac agaaatggag agcccgtaga
     1381 gtatgccacc ggtcctgtta tttggggaga acctggtaca aacggacaac atgcttttta
     1441 tcagctgatc caccagggca ctcctttaat tccctgtgac ttcattgcac tggcacaatc
     1501 ccataatcct attggagatc atcatataaa attgctgagt aatttttttg cacaaacaga
     1561 agcattgatg aatggcaaaa cagaagatga agcagaaaag gaattagaaa aacagggatt
     1621 aaatgcagaa gacattgtca aattacttcc ttataaaatc tttaaaggca acaggcctac
     1681 caattcattt ttgataaaaa aactgacccc ttacacagta gggcaattgg tagctttgta
     1741 tgagcacaaa atatttgtgc agggtgttat ctggaatata tacagctttg accaatgggg
     1801 tgttgaactg ggcaagcagc ttgccaataa aatattaccc gaactgcaga ctaatgtacc
     1861 agtcattgac catgattcat caactaacgg acttatcaat gcatggaaac agatgaaaaa
     1921 ataattgtac tttcaatgta caaacctgtt ttatgaaaac gatatttctt ctgtttggtt
     1981 tatttggctg ctatggagtt tctgcccagc aaaaagacct ctttgatatt gaaaagcacc
     2041 tgcataaaaa atcaattcaa aaaaaaggaa caaaacacag gcttctgcaa cgagataatt
     2101 ttaataatta taaatctgtt ccagaaaaaa atccggcaaa taaaagttat accggcgttt
     2161 tatcttatac tctaccaaac ggtgataaat tgtatagtct cccaacggat aatatgccct
     2221 gtgtggtgcc ggatatgaat cagtataata ttatgccgaa tgcgggaact agtacaatag
     2281 taatactcca aaatcgtccg gaaatcatcc ccaatcctgc taagccgttt aaaataccag
     2341 cgacaaaata attagtaaaa aattaaagag gctgtctaaa atagtatgtc agcctgcctg
     2401 ccccgacgaa tgtcgtggga gcttgttgaa ggctaccgtt ttgattgcca atatttagat
     2461 atccagatag ctatcggttt gattgtcata ttgagtttat cgaaatattg gcaatcaaac
     2521 catcttttga gacagcaact tttcttttcc tgcttcttct ttcactttat tactaactaa
     2581 aatttgtttt caatagtaag tataaaaaaa gccctccgtg attagcggag ggctttgtat
     2641 tgagtgtgtg actctgtttg tacttcctct tcttattgta ccagtacaag gcctgatacc
     2701 actttgcccg taaacatatt ggcgactttc accacatacg ttcctttcgc ttttacacca
     2761 cccaggtcaa agtccatacg catatatggc gctgtgcctg ataccagtgt aaactcacga
     2821 ctggtgatca cctggcccaa tggattgtaa atacttacca ctctcttctc ggccaaacct
     2881 ccctcataat acaatcttac ctggaaggca cccgtggtcg ggttcggata gatccacaac
     2941 ttatctgacg cctctgatcc gatcaccagg gtattggaac tggacacaca accattcaca
     3001 tccgttacgg ttgcctggta tgatcctact ccatctatat ttaccacctg gctattcgtt
     3061 gtgccggaca gacctgaacc attcaatgtc catgaccagc cacctgctgc tgcagccggt
     3121 gtacttgtac cagttatggt cgtcgtacga cccggtacca ggttcactac cggtgatgac
     3181 aaggtcaccg ttggcaacgg atttaccgtc agtgtgccaa aggcagttga agttacagcc
     3241 ggtgtacatg gagctgacgt tactacaaaa cggtaacggt ttccactcat tgactgggta
     3301 acactgctta ctgtaagtgt tgaactggta gctccgctga tattattata ggtaagacct
     3361 ccgtccgtac ttaactgcca ctggtaagct atcgggttac ctcctgtagt ggaggccaca
     3421 aacgtggtgc tgccacccaa acatactgca cgggttgacg gggttaccgc aagacctgtc
     3481 gcaggatggc tcacatttac cggaactgtt gtcgtggctg acgtacacgg agtcggcgtg
     3541 gtaaagctta ctgtatagtt attcgtattc gctgtcggct tcacatatac catagtcgcc
     3601 ggagtacctg tataggctat cgtagcagcg gcatctgtaa agatcgtacc ggcaggacct
     3661 gcccatactc cctgtgcaaa tacaggtgct acataagtaa tagaaattga ccatgacgtt
     3721 aacgtcccca catctcctcc accaaggtca cacattgcca gcgtccagtc gccattcatc
     3781 gttcccagca acggtgacca gaagttcgtc gttgtcggac ccactgtcgg aaccgtagcc
     3841 gtaaacctgt cggcacggaa ggaacctgtc cttggtgcag gagctccgct catggctgtt
     3901 gttgaaatag aatctactac ggtattgaca aaatcatccg taccgttgga acccgttccg
     3961 ccatccagca gacctatcag gttcagcgtc tgaccattcg gcgctttcag gttcatcacc
     4021 aggtcgccta cccaggtatg cgccatatta aaccggattc ctacattggt aattacagat
     4081 cctgcaggga taccggatac agctacgctg ttcgttactc ccgtcggatc attatcagtt
     4141 actactaacg gcaatcctgc tgatgcattg aaagttactg tcgtcgcggc tgatacacta
     4201 ttggtcagcg tcaacgcctg gatagtgcct tcacatatcg tcgcagaggc aggattcacc
     4261 acaggaacta tcgcattcac tgttaaagta gccgggggac ttgtaaagtc tactgcagaa
     4321 caaccaccag tcactaatgc acggtactgg tatccattat aatcagctga cacattagcc
     4381 aatgacaacg ttgcagtagt tgcaccactg tatataccac cattggtcac agtttgccat
     4441 actccggatg aacttgtacg atactgccag gaataagaag gaagacttcc ggaagcagct
     4501 accgtaaaac taacattatt accacaggta actgatgtac ttgatggtgc acttgtaata
     4561 gttactggta tacatggtgt tattgttaca ctataatctt cggtttcact gaaagtagat
     4621 ccagtacaag cttgtgcatt cgtaaatact ccaccaaatc gtatccttac cctcatacgg
     4681 gttgtaccat ttaaagctgt tgaaggaatt gctatactac ctgtaactga agtctgacct
     4741 gcaggcttat taccaaggct tgtatactcg gtagtttcaa actggccatt ttgattgtaa
     4801 tcaatccaaa cacatattcc ttcggtccat gtagctggag catttactgt aatcgggtta
     4861 gctgcacctg agaatactga cggagctgct accgttgtag tataatttga atatccagtg
     4921 ggagggccgg cactacatcc ggatgaatta tccagggtac tgatacgaac tcttgtaatc
     4981 acatcatcat ctgtacaatc tgatgccggt ggtgtacaat aacagttcga tggtggggtt
     5041 aatgccactg taacaggagt tgaagtaccg gtattacctg agcaggttac attgcaacga
     5101 tattgtgtag tagctgtcaa tgttgttgtg taagaagagt tggtcgcacc ggcaatattt
     5161 gtccaggtag taccatccgg tgatgattgc cactgataag taacgcctga accggctgta
     5221 ccattctgaa gagataacgt aaagtttata ccaggacaga cagtagatac agttgataat
     5281 gtattccccg gagcaggtgt gcctgtacat gcacctgttg tataatcaat aataaagggg
     5341 aaatcctggg gattcgttcc gtcattagca tttgcccagg tattacccgc cagggcatgc
     5401 tgtaaagcat tattacctgg ttgtgtggta gtccctacaa cagtagatgc aggagagaaa
     5461 ttactcagtc ctccattacc aacaccccat tgtatccagt aagtaccagc attaagtaca
     5521 gtagctactg ttgcttctat tttccatatt tgccttgtag taccgggtgt tgcattaaaa
     5581 attctgtaca tacttgctgt acttgatgca agaaacctgt tagtggtcaa atcaccataa
     5641 ataggtgctg gattacccac tgatgggtta gtgttgtaaa tagctactct aacatcagtg
     5701 taaggtgaag ttggtcctgc aaatccggta gaataagcat aaaaagtaat cttagtaaga
     5761 ttccagctac cggctgcaat tgtaaaatca tccgccagca taaaatcagt aatattatta
     5821 ccaaacccgg cagttccatt gccagcctga acctcactcc aggtaaatcc tgcaggggca
     5881 gcaacaccat tacttgatgt tgcacctgta ctcaaattac catttacata ttgttgggaa
     5941 tttatttttc ccgcaaccag gaaaaataaa atgagaaaaa gtggagaaag cccaaataaa
     6001 tttctccgcc tttgattaag cgtaaatttt gattgcatac aaacagtttt ttggttgata
     6061 agtactcccc gggaagggag aaaatccata atccaaacgt aaggtagttt ttttttacaa
     6121 tttaaaaaaa ctgtgttctg atcataaaaa aagccctccg tgattagcgg agggctttgt
     6181 atagagtgtg tgactctgtt tgttttactt cctcttctta ctgtaccagt acaagacctg
     6241 ataccacttt gcccgtaaac atattggcta ctttcaccac atacgttcct ttcgctttta
     6301 caccacccag gtcaaagtcc atacgcatat atggcgctgt gcctgatacc agtgtaaact
     6361 cacgactggt gatcacctgg cccaatggat tgtaaatact taccactctc ttctcggcca
     6421 aacctccctc ataatacaat cttacctgga aggcacccgt ggtcgggttc ggatagatcc
     6481 acaacttatc tgacgcctct gatccgatca ccagggtatt ggaactggac acacaaccat
     6541 tcacatccgt tacggttgcc tggtatgatc ctactccatc tatatttacc acctggctat
     6601 tcgttgtgcc ggacagacct gaaccattca atgtccatga ccagccacct gctgctgcag
     6661 ccggtgtact tgtaccagtt atggtcgtcg tacgacccgg taccaggttc actaccggtg
     6721 atgacaaggt caccgttggc aacggattta ccgtcagtgt gccaaaggca gttgaagtta
     6781 cagccggtgt acatggagct gacgttacta caaaacggta acggtttcca ctcattgact
     6841 gggtaacact gcttactgta agtgttgaac tggtagctcc gctgatatta ttataggtaa
     6901 gacctccgtc cgtacttaac tgccactggt aagctatcgg gttacctcct gtagtggagg
     6961 ccacaaacgt ggtgctgcca cccaaacata ctgcacgggt tgacggggtt accgcaagac
     7021 ctgtcgcagg atggctcaca tttaccggaa ctgttgtcgt ggctgacgta cacggagtcg
     7081 gcgtggtaaa gcttactgta tagttattcg tattcgctgt cggcttcaca tataccatag
     7141 tcgccggagt acctgtatag gctatcgtag cagcggcatc tgtaaagatc gtaccggcag
     7201 gacctgccca tactccctgt gcaaatacag gtgctacata agtaatagaa attgaccatg
     7261 acgttaacgt ccccacatct cctccaccaa ggtcacacat tgccagcgtc cagtcgccat
     7321 tcatcgttcc cagcaacggt gaccagaagt tcgtcgttgt cggacccact gtcggaaccg
     7381 tagccgtaaa cctgtcggca cggaaggaac ctgtccttgg tgcaggagct ccgctcatgg
     7441 ctgttgttga aatagaatct actacggtat tgacaaaatc atccgtaccg ttggaacccg
     7501 ttccgccatc cagcagacct atcaggttca gcgtctgacc attcggcgct ttcaggttca
     7561 tcaccaggtc gcctacccag gtatgcgcca tattaaaccg gattcctaca ttggtaatta
     7621 cagatcctgc agggataccg gatacagcta cgctgttcgt tactcccgtc ggatcattat
     7681 cagttactac taacggcaat cctgctgatg cattgaaagt tactgtcgtc gcggctgata
     7741 cactattggt cagcgtcaac gcctggatag tgccttcaca tatcgtcgca gaggcaggat
     7801 tcaccacagg aactatcgca ttcactgtta aagtagccgg gggacttgta aagtctactg
     7861 cagaacaacc accagtcact aatgcacggt actggtatcc attataatca gctgacacat
     7921 tagccaatga caacgttgca gtagttgcac cactgtatat accaccattg gtcacagttt
     7981 gccatactcc ggatgaactt gtacgatact gccaggaata agaaggaaga cttccggaag
     8041 cagctaccgt aaaactaaca ttattaccac aggtaactga tgtacttgat ggtgcacttg
     8101 taatagttac tggtatacat gtaaccaggt tcacattata atcttctgtt tctccaaaga
     8161 atccaccggt acaagcttgt gttccggtta atgctgtgct ccaacgaact cttacccgca
     8221 tcctcgttgt acctgtaagg gccgatgccg gtacagcaac aataccatta atagttgatc
     8281 cattaccact cccaagcgca gtaaattctg tggcttcaaa ctggccattc tggttgtaat
     8341 cgatccaaac tgccacatgc tctgttccac caggacctac acttacagcc atagggttag
     8401 cagcaccaat aaatacatca ggtgcagcaa ctgctgaata atatgtataa ccggcgcctg
     8461 aacaacccga agaaggatta ttaagtgttc ctaatcttac attcaatatc tcatcatcca
     8521 acgtacacgt cgttgcagcg ggtgtacaat aacaggctga tggagcagcc atattcactt
     8581 gtaatgcagt agatgtaccg gtattagcac cacaggtcac attgcatctg taataagttg
     8641 caacagtttg tgaggttgtc aatgttccat tagtagcacc tgtaatatta gtccaggtag
     8701 taccatccgg tgatgattgc cactggtacg taacaccaga acccggtgta gctaatgata
     8761 aagtaaaatt gatacccggg caaacagggt tggccgtact atttgtagct cccggagcag
     8821 gtgtacccga acaggcaccg attgcagtat aaacaacttc atctatgccg atgtaattag
     8881 aattagcacc ggaaggacct ccgttttcta cgaaatagcg aaatgctaac ctgccggaag
     8941 ttggtgcagg tactcctgaa atgataacag taaattgttg ccatgtttca ggatatgcag
     9001 ccccgtaggt aggattaata tccaataata aattagtgaa gtctccgaca gaagtggcag
     9061 ttgctcccgc atttgtactg gcaccattaa gactcattct tacctgtaag cgatcgggga
     9121 aagtagcacc accagtcggc acccttgtat agaatgtaaa ctggtcaccg ttcttaattg
     9181 agatgttcgg cataaacagc cagttgctga tcgtattagc accagtagta ttattaaaat
     9241 tggcgccaat atatgctgtc gcagctccgg cttgcgaagg aaaaacacca gaatttccct
     9301 ggaaccagcc tgtgctacca accggagaac tcaggttttg attggcccag cccggtagta
     9361 cagtaatatc agtaaatccc tcactataga ggacctgagc agttgatgtc tttgacaaag
     9421 acaagaaaat taatgccatg gcaaaaattc tcgtcagtaa aaacatcttt tttttaagtg
     9481 taaattttga ttgcatattt cccgttttaa atttgaataa acacaaaagc cccttgcata
     9541 aagcaacggg gctttaaaat tttctaaaat ttccagtaaa caagtaaatg taactatttc
     9601 tcattcgcct gatttggttg ccataaaggt attgtattca aataaaaata cccgccagag
     9661 caggtatatt tattttacgc tattttatta ccatttatcg acctcttcac cgctggtatc
     9721 cgggctggta tttgtttcag ccaccggggt ttcggacaca acatcacctg cagattcaac
     9781 gactggtgct gcttcctggc taacctctgc cccattctga ttatactcat ccgggttctc
     9841 gtcatggttg aaagcatcaa aatcaaagtc aggcatcaat tctgttttca catagtcaac
     9901 ggcctcaccc aaagctttaa taaacttgtt gaaatcctct ttgtaaagaa aaaccttatg
     9961 acggtcatag ccattgtcat caaatcgttt ccggctttca gttattgtga ggtaataatc
    10021 atttccgcgg gtagctctta catcaaagaa gtaagtcctt cttttacctg cacggatgcg
    10081 tttactgtag atactctcca ttttcttgtc gttattctcg tacgccactg tataagtttt
    10141 tagcagttaa tgaattactt tagttttgga agctacaaat atagatatgt tttcgaaacg
    10201 gccaaattga caaaaaatct ttatttagcc aatatattgc ctgcaaaaaa ccggaaatat
    10261 tgctccccaa ttgagaatct gactgccctt ttaatttgtg ccccgatcct gttgctgctg
    10321 ccggaggtat aattttgcat aatagccctt ttgctgaata agctcatcgt gggtaccctg
    10381 ctctattatc cggccggctt ccagcactac gatcttatca aaattgagca aagaaaaaat
    10441 cctgtgtgtt ataatgatcg ctgttttctg atgcaggtaa tcattcaggt ttgaaataat
    10501 ctctttctct gttcgggcgt caaccgcact caggcaatca tcaaacacta caatatttga
    10561 gtctttgatc aatgccctgg caatagtaat ccgttgtttt tgtccgccgc tgagtgtcac
    10621 tcccctttca ccaatcattg tatcgtattg cccggaaaaa ttggcaattt ccttttctac
    10681 acttgctttt atagctgcat tttctaccgc atttctatcg aactgcatca ttccgaaacc
    10741 aatattgttt tctaccgttt cgctgaacag aaatacatcc tggggaacat agcttatttg
    10801 ctgccggagt actttcaaat ctattttact gatattatta ccatccattt caagtgttcc
    10861 gtctgtcaca tcatacattc tcagcagtaa ttgcgcaagg gtcgacttcc cactgcctgt
    10921 cctacctaca atagctactt tctcaccact ccggatatgt aaactgaaat ctttaattgc
    10981 ttctataccc gtgtggggat aggtaaaact tactttgtca aatttaatat cgccctgtaa
    11041 gggaatgttg agtgctgcag gctggtttcg aatgccgggt tttatatcca aaaactcatt
    11101 gatccttttt tgtgaagcag cagcccgctg gatcatactg gcggtaagac caatggcact
    11161 caccgggaag gtgagcatgt taatatacat gacaaattca actattgtgt taataccaat
    11221 attatgcccc ccgtaaatat aatataaccc tccgatcatt attgtcagta atgtactaag
    11281 gccaataagt aaggttatag aaggaaaata aatagcttcc actttgacaa ggcccagtac
    11341 atttttttta aaatcttctg cagtgcgggt aaaaaaacca agcattgcct tttcctgcac
    11401 aaatgacttt ataaccctga ttccagaata ggtttcctgg gcatttgtgg taagatccga
    11461 gagtga
//
LOCUS       DAORTI010000113        11454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5821_length_11454_cov_5.501886, whole genome shotgun sequence.
ACCESSION   DAORTI010000113 DAORTI010000000
VERSION     DAORTI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11454
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5821_length_11454_cov_5.501886"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLO70_12800"
     CDS             <1..287
                     /locus_tag="PLO70_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HQZ75391.1"
                     /translation="VHVMWVCPGFTTSNIRNAALNKDADSHGETPMDESKMMPAEECA
                     AHILTAIRKKKRTLVLTFTGKRTVFLNKFFPKLTDNMVYKFFFKDGKLVK"
     gene            340..1149
                     /locus_tag="PLO70_12805"
     CDS             340..1149
                     /locus_tag="PLO70_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HQZ75392.1"
                     /translation="MPLLTLTSDIGNPDYLVGAVKAQLLQTNPAFQIIDISHNILLFN
                     YSQAAYVCRSAIKNFPDFTYHLLLVNLFEKKPEQLLLAFHNNQYFICADNGLLTMILE
                     DNPEIIIGIPLDKAAIKNTIYLTSVMGNIINQLVNGESIKNIGLPDVQYLEKRHLRPM
                     LDNNSIEGQIIFIDSFENVIVNITREQFEEQRKGRGFKIIFKRDETIDHISESYADVP
                     EGEKLALFNSAGYLEIAINKGNAAGLFGLKGFSEKMRQGQLSYQTVRVYFE"
     gene            1347..4877
                     /locus_tag="PLO70_12810"
     CDS             1347..4877
                     /locus_tag="PLO70_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQZ75393.1"
                     /translation="MNFRKVNNITGWAIFLIALATYFLTREARGSLWDTGEFIASAKG
                     VQLPHPPGAPLFVLLGRFFIILFGDNGQTAASAVNFMSALASAATILFLFWSITHFAR
                     KMFVSAGEQLTAQQTFTTMAAGAVGALAYTFSDSFWYSAVEGEVYALSSFFTALVFWI
                     MLKWEHADEHAGNDLDARAKSDRWIVLLFFMMGLSIGVHLLNLLTIPAIVMIYYYRRY
                     EVTTKGAIVAFLIGCLITGIVQVGVIQYSMKAAGQFDVFFVNSFGLPFFSGFAIYFIL
                     LAILILWALRFKEKSLSHTKLIIWFVLYLFFSALPFVIVPGDGLKILPFLLLIGGAVA
                     FGYFLKPTALKVIKLSLWCYAFIMLGYFMYFTALIRSNANPTIDMNNVDNPISLVYYL
                     SREQYGSAPIVYGPHFAASVPEDLDEEQPLIESEMKYVKGENKYVPIGRTKEYNFKSD
                     VKQLFPRIWDSSDDQYHAQFYAEWLGLGQDPQTGKYEAPSYGDNIRWFFGYQMSHMYW
                     RYFMWNFAGKQNDIQGLGNKRDGNWISGFSFIDNKRLGDQSQLPDSIKNNKAHNQLYL
                     LPFILGIIGCVYQYFKNNKDWIVTFLLFFFTGIAVVLYLNQPGNQPRERDYAYVGSFY
                     AFAIWIGLAVVALVRMAREKADQLTFKNMLTYGSVLTFAITFMSCFPGSGDGALMTSL
                     FATVLYAAVTAGVTFLARAISAAGKNERIVNLSTTVVCLAVPVLMAQQVWDDHDRSNK
                     TTAPDIAKDYLESCAPNAIIFTFGDNDTYPLWYAQEVEGVRPDIRVINNSLLGIDWYI
                     NQLRYKVNEADSIDMIWDADKIMGNNRNYVVYSPKPGISQNAFYDLYDMMKNYVGSDD
                     PSKMDNSRGEPLNAFPVTKVTVPVDREVVLKNGTANPTDSILTEFRFELPQRALMKND
                     LIILNIIAANKWKRPIYFTSPIGELGFGQYLRKDGIAYRLVPVVNKFSQMNWVVDQSL
                     RQNGLGGTQIRDNNSDLMFKNLMTKYEFGGADKKGVYFDEENRRHILNIRAIFGEAAG
                     NLADIGRKEDAQKLIDKVETGINPENLPYAMASRYNSHNQTALVYLEACYKAGKTELA
                     EKVRKEVRKDLEQQAKYYNYIRDNKPDFWGGFERSEAPINERLLRVLGDIEARYAPQT
                     QTKPVNEGPTTIQNTAKPDSNQAKDSGNQP"
     gene            5004..6101
                     /locus_tag="PLO70_12815"
     CDS             5004..6101
                     /locus_tag="PLO70_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQZ75394.1"
                     /translation="MKGFHFTKFDPDSEGKSRFEQLLDLFMQLLTYTSGDVSEAMQWM
                     NELDKKYQLTDDDYGMGDFIDELKEKGYITGDNEQGQIKITPKTEQGIRKKSLEEIFG
                     KLKKTKQGDHHSFKPGQGDELNPETRQFQFGDMLEQIDFTESIRNAQINHGIDSFRMQ
                     EDDLSIRETDFKAQTSTVLMIDISHSMILYGEDRITPAKKVAMALSELIKTKYPKDTL
                     DIVVFGNDAWPIEVKDIPYLQVGPYHTNTVAGLELAMDILRRRKNPNKQIFMITDGKP
                     TCLKIGKRYYKNSFGLDRKVVNRCINLAAQCKKLKIPITTFMIATDPNLQKFVQEFTE
                     TNNGKAFFASLDKLGAFIFKDFESGKRKTVY"
     gene            6189..7715
                     /locus_tag="PLO70_12820"
     CDS             6189..7715
                     /locus_tag="PLO70_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQZ75395.1"
                     /translation="MNIKKIKTLGELKASGYQPKSIKEEIRQNLIAKLQKNEPTFSGI
                     VGYEDTVIPDVERALLSKHNILFLGLRGQAKTRMARQMTELLDEYIPCVAGSDINDDP
                     VNPVSKYAQEEIATHGDETAIHWIHRSERYGEKLATPDVSVADLIGDIDPIKAANLKL
                     SFADERVLHYGIIPRSNRGIFVINELPDLQARIQVALFNILEEGDVQIRGFKLRLPLD
                     IMFVFTANPEDYTNRGSIVTPLKDRIESQILTHYPKSLENALEITEQEAEISADQKAK
                     VKVSDLIKRLIEQVAFEARSSEFVDKKSGVSARLTISAFENAVSSAERRAIINNEKQT
                     QVWMSDLVGIIPSITGKVELVYEGEQEGPYQVAMNLVDKAIRSQFAQYFPNPDTLKKR
                     RNTGKPSQEEQKDDNPYRPITGWFDKGNNLNLSFNTSDKDKMLLLYKVDGLNALVKKY
                     FPKSNEEQAALLMEFVLHGLSSYSLISKKIFENKIEFKDLVGSMMNFSEKDFEFDEEA
                     "
     gene            complement(7781..8791)
                     /gene="murB"
                     /locus_tag="PLO70_12825"
     CDS             complement(7781..8791)
                     /gene="murB"
                     /locus_tag="PLO70_12825"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQZ75396.1"
                     /translation="MLLQENISLKPYNTFGIDVKARYFATFNDTDELAETLNLKSQTS
                     NLILGGGSNILLTKDYDGLVLKNEIRGIVELHEDSEYVYVKAGAGENWHQFVLYCIER
                     NWAGVENLSLIPGNVGASPMQNIGAYGVEIDDVFWDLEAFHKKDKKVVTFTRSDCEFG
                     YRESVFKEKFKDQFVILNVTFQLRKKPRFNTSYGAIEQELQRMGVRELSLKAISAAVI
                     NIRNSKLPNPGEIGNAGSFFKNPSVTNEQFVSLKARFENMAGHENIDGTVKLAAGWLI
                     EQCGWKGYRKGDAGCHAKQALVLVNYGNATGTEIYNLSEEIIQSVKEKFGITLEREVN
                     II"
     gene            8880..10253
                     /locus_tag="PLO70_12830"
     CDS             8880..10253
                     /locus_tag="PLO70_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQZ75397.1"
                     /translation="MKKVFLLLLLPAFIISCKSKAPKTENTTETVVATHPEWIMQGNI
                     YEVNVRQYTSEGTFNAFAQHLDRLKNMGVQTLWFMPVNPISKVDRKGTLGSYYAVADY
                     TAINPEYGTMDDWKKLVTTIHEKGMKVLIDWVPNHTGADHPWLTTHPDFFVKDSTGKA
                     AIMSDWTDTRVLDFKNNEMQDSMIAAMKFWVTQTGIDGYRVDVAWNVPGEFWKKCIPQ
                     LKAVKNDLFFLAEGDKPYLMISGFDAYYPWEMFVMMTKVAAGERPAFALDSVKAKYDS
                     VYPKEAIPMYFTSNHDENSWNKSDYGTFPGIVHAPFAVFSQTMGGTVPLVYSGQEEPL
                     LRPLKFFEKDPIVFGKFERAKFYKSLHDLRKKNPALAADASFKKVNLGNDTTIYAYVR
                     EKGNHKVLVILNLTKKEQDIKITDKSLQRLAYNIFTGVNEQLSEKRKSLSPWSYFVYE
                     YPVAGAN"
     gene            10300..10689
                     /locus_tag="PLO70_12835"
     CDS             10300..10689
                     /locus_tag="PLO70_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HQZ75398.1"
                     /translation="MRATVIGATGLIGSHLLELLLADDNFDSVRILLRRPFDLTHPKL
                     EKKLVDFSDMESFRLALEDSDVIFCAIGTTQKKVKGDKSAYRKVDFDIAVNSAQLGKL
                     CGCETFVLVSAVGANSQSNNFLPETER"
     gene            10703..10912
                     /locus_tag="PLO70_12840"
     CDS             10703..10912
                     /locus_tag="PLO70_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75399.1"
                     /translation="MKATGIKSIHIVRPSLLLGDRKEFRLGEKMAQWMMPVFLFLIPK
                     KYRPIHAKKVAEMMINAAKITGPNR"
     gene            complement(11038..>11454)
                     /locus_tag="PLO70_12845"
     CDS             complement(11038..>11454)
                     /locus_tag="PLO70_12845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQZ75400.1"
                     /translation="ILIKKMGAVKAIFFSAAAFGMYHWFSHELWGNPVQMAIEFFTSG
                     AMGLVLAYGYAKTFSLYVPVAIHFGWNIVQMVVFSGNTIGEQVFIEVLPRPVITVSYF
                     NFFVMLFLPLLCCLAINGWMLKRYKQAEPPGAGNNT"
BASE COUNT         3596 a         2321 c         2382 g         3155 t
ORIGIN      
        1 atgtacatgt aatgtgggtt tgtccgggtt ttaccacctc gaatatccgc aatgccgcac
       61 tgaacaaaga tgctgactca catggcgaaa cacccatgga tgaaagcaag atgatgccgg
      121 ccgaagagtg cgctgcacat attttaacgg ccattcgtaa aaagaaaagg acactggtgc
      181 tgacatttac gggtaaacga acggtgttcc tgaataagtt tttcccgaaa ctgacagata
      241 acatggtcta taaattcttc tttaaagacg gaaagctggt gaagtagaat cgtaatattc
      301 ctctttacta tttttatata gattattttg catgcctaaa tgcctttact aacccttaca
      361 tcagatattg gaaaccctga ctacctggtg ggtgcagtaa aggcacagct attacaaact
      421 aatcctgcgt ttcagatcat tgatatttcg cacaatattc ttctctttaa ttattcgcaa
      481 gctgcatatg tatgccggag tgcaataaaa aatttccccg atttcactta ccaccttcta
      541 ctggtaaacc tgtttgagaa aaaacctgaa cagttattat tggcgtttca taataaccag
      601 tattttattt gtgcagacaa tggcctgctc acaatgatat tggaagataa tcccgagatc
      661 attatcggca ttccacttga taaagctgca ataaagaaca ctatatacct gacatcagtt
      721 atgggtaata ttattaatca attggtgaac ggggaatcaa taaagaatat tggtttgccg
      781 gatgtacaat accttgaaaa aagacacctg cggcctatgc tggacaacaa ttccatagaa
      841 ggccagatca tatttatcga cagctttgaa aatgtgattg tcaatattac ccgtgaacaa
      901 tttgaagaac aacggaaagg caggggtttc aagatcatct tcaaacggga tgaaacgatt
      961 gatcatatca gcgaaagcta tgcagatgta cccgaaggag aaaaactggc attattcaat
     1021 agtgccggat acctggagat tgcaataaac aaaggcaatg cagccggatt atttggattg
     1081 aaaggcttct ctgaaaaaat gaggcaaggc cagctctcct atcaaaccgt aagggtttat
     1141 tttgaataaa tagtatttat tccccttgta ttactcatca aaaccctctt gtttacactg
     1201 gcatatactt agcgaaaatc aggtttattc taaaattgct aaaactggat ttcgggaaca
     1261 aaaatctacc cctgaaaccc tatttttgct gtctcccggc cacgaaagct atcgtggcct
     1321 gagggaacta tcaaaatcaa ccatttatga acttcagaaa agtcaataac attacaggct
     1381 gggctatttt tcttattgca ctggcaacct attttcttac ccgtgaggca agaggaagtt
     1441 tgtgggatac aggtgaattt attgccagtg ccaaaggtgt acaattaccc caccctcccg
     1501 gtgcgccatt gtttgtgttg ttaggccggt tcttcattat actctttgga gacaatggcc
     1561 agacagctgc cagtgccgta aactttatga gtgcattggc cagtgctgcc accattttat
     1621 ttctcttctg gagtatcact cactttgccc gtaaaatgtt tgtaagtgca ggcgaacaat
     1681 taaccgcaca acaaactttt accacgatgg ctgcaggtgc agttggtgca ttggcatata
     1741 cattcagtga ttcattctgg tacagtgccg tggaaggtga agtgtatgca ttatcctcct
     1801 tttttacagc attggtgttt tggattatgt taaaatggga acatgctgat gagcatgcag
     1861 gcaatgacct ggatgcaagg gctaaaagcg acaggtggat cgtactcctt ttcttcatga
     1921 tgggactttc aataggtgtt cacctgctga acctgttgac catccctgcc attgtgatga
     1981 tttattatta caggcgttat gaagtaacta ccaaaggtgc tatagttgcc tttttaatcg
     2041 gctgcctcat aacaggtata gttcaagtgg gagttattca atactcaatg aaagctgccg
     2101 gtcagtttga tgtattcttt gtcaattcat ttggactacc cttcttctct ggttttgcta
     2161 tttatttcat cctgttagcg attttgattt tatgggcact ccgctttaaa gagaaatcat
     2221 tatcccatac aaaacttatt atttggtttg tgctctacct gttcttctct gcactgccct
     2281 ttgtaattgt tcctggtgat ggtctcaaaa ttttgccttt ccttttactc atagggggtg
     2341 ctgttgcttt tggttatttc ttaaaaccaa ccgcattaaa agttataaag ctttcgctgt
     2401 ggtgctatgc ctttatcatg ctgggatact ttatgtattt caccgcactt atccgttcca
     2461 atgccaaccc gaccattgac atgaacaatg ttgacaaccc gataagcctg gtttattatc
     2521 tcagccgtga acaatatggc agcgccccga ttgtgtatgg ccctcatttt gccgcttctg
     2581 taccggaaga tcttgacgaa gaacaaccac ttattgaaag tgaaatgaaa tatgtgaaag
     2641 gggaaaataa atatgtaccg atcggcagaa caaaagaata taatttcaaa agtgatgtaa
     2701 agcaattatt tccgagaatc tgggactcaa gcgatgacca ataccatgct caattctatg
     2761 ccgaatggct tggactgggt caagacccgc aaaccggaaa atatgaagct ccttcctatg
     2821 gtgataatat aagatggttc tttggctacc agatgagtca tatgtactgg cgatacttta
     2881 tgtggaattt tgcaggtaaa caaaatgata tacagggctt gggtaataag agagatggca
     2941 actggataag cggcttctcc tttatcgata ataaaagact gggcgatcag tctcaattac
     3001 ctgatagtat taaaaacaat aaagcgcata atcaattata cctgttgcct tttattctgg
     3061 gtattatcgg ttgtgtgtat caatacttta aaaataataa agactggatc gtcaccttcc
     3121 ttctcttttt cttcactggc attgccgtag tactttacct gaaccaacct ggcaaccagc
     3181 cccgtgaacg ggactatgcg tatgtaggtt ctttctatgc tttcgccatc tggataggtc
     3241 ttgctgttgt tgccctggta agaatggccc gtgaaaaagc ggaccagtta actttcaaga
     3301 atatgcttac ctatggcagt gtattgactt ttgctatcac attcatgagt tgcttccccg
     3361 gtagtggtga cggagccctg atgacatccc tatttgctac tgttttatat gctgctgtta
     3421 cagccggagt tactttcctc gcaagagcta tctctgctgc aggaaaaaat gaacggatag
     3481 taaatctgag cacaacagtt gtttgccttg ctgtaccggt actgatggca caacaggtat
     3541 gggatgatca tgacagaagt aataagacca ctgccccgga tatagcaaag gattacctgg
     3601 aaagctgtgc acccaatgca atcattttca cttttggtga taatgacacc tacccgcttt
     3661 ggtacgcaca ggaagtagaa ggagtacgtc cggatatccg tgttatcaac aatagtttac
     3721 tgggtattga ctggtatatc aaccagcttc gttacaaggt taatgaggct gattcaatag
     3781 atatgatatg ggatgcagat aaaataatgg gtaacaacag gaattatgtt gtgtacagcc
     3841 cgaaacccgg tatctcacaa aatgctttct acgacctcta tgacatgatg aaaaattatg
     3901 taggaagcga cgacccttct aaaatggata actcaagagg tgaaccactg aatgcctttc
     3961 cggtaactaa agtaactgtt cccgtcgaca gggaggttgt attgaaaaac ggaacagcaa
     4021 accctacaga cagtatcctg actgaattcc gttttgaatt accacaaaga gcattgatga
     4081 aaaatgacct gatcattttg aatatcattg ctgccaacaa atggaaacgt cctatctatt
     4141 tcacctcccc gatcggtgaa ctcggctttg gacaatacct ccgtaaagat ggtattgcct
     4201 atcggcttgt tccggttgtc aacaaatttt cccagatgaa ttgggtggtt gaccaatcgc
     4261 ttcgtcagaa tggattaggt ggcacccaga taagggataa taacagcgat ctgatgttca
     4321 aaaacctgat gaccaaatat gaatttggcg gagcggataa aaaaggcgtt tattttgatg
     4381 aggaaaaccg ccggcacatc ctgaatatta gagccatatt cggagaagcg gcaggcaacc
     4441 tggcagatat cggacgaaaa gaagatgcgc agaaattgat tgacaaagtt gaaacaggga
     4501 taaacccgga gaatcttccg tatgcgatgg cgagccgtta taatagccat aaccaaacag
     4561 cgcttgttta cctggaagct tgttataaag ccggaaaaac tgaactggca gaaaaagtaa
     4621 gaaaggaagt acggaaagac ctggaacaac aagcaaaata ttacaattat atcagggata
     4681 acaaacctga tttctggggt ggctttgaaa gatctgaagc gcctatcaat gaacgattat
     4741 tgagagtact gggagatata gaagcaagat atgcaccgca aacacaaaca aaaccggtta
     4801 acgaaggccc caccacaatt caaaatacag ccaaaccgga tagtaaccag gctaaagatt
     4861 caggtaacca accttaattc attgccaata ttgttaagaa aaaccctcct gctatggagg
     4921 gttttttctt ttaaacttat aacagtttaa gttgttgtta agatgatctg acagcaaggt
     4981 atttgtcgta aattgtatgt tcaatgaaag gttttcattt cacaaaattt gatcccgata
     5041 gtgaaggtaa aagcaggttt gagcaattgc ttgacctttt catgcagtta cttacttata
     5101 ccagtggtga tgtaagtgaa gccatgcaat ggatgaatga attggataaa aaataccagt
     5161 tgaccgatga tgattatggt atgggtgatt ttatagacga actgaaggaa aaaggttata
     5221 tcaccgggga taatgaacag ggacaaataa agatcactcc taaaacagaa cagggcatcc
     5281 gtaaaaaaag cctcgaagaa atttttggta aactcaaaaa aacaaaacag ggcgatcatc
     5341 atagtttcaa acccggtcaa ggggatgaac tgaaccctga aacaagacag ttccagtttg
     5401 gcgatatgct ggagcaaatc gatttcacag aaagcatccg taatgcacag atcaatcatg
     5461 gtattgacag tttccggatg caggaggatg atcttagcat aagagaaaca gattttaaag
     5521 cgcagacatc caccgtactg atgattgaca tctcccactc catgatcctg tatggcgagg
     5581 acagaattac tcctgctaaa aaagtagcga tggcattgag tgagctgata aaaacaaaat
     5641 atcccaagga cactttagat attgttgtat tcggtaatga tgcctggccg attgaggtga
     5701 aagacattcc ctatttacag gttggtccct atcacaccaa tactgtggcc ggattagaac
     5761 tcgccatgga tattctgcgg agaagaaaaa acccgaacaa gcagatattc atgatcaccg
     5821 atggtaaacc aacctgttta aagatcggca agcggtatta caagaacagt ttcggacttg
     5881 acaggaaagt agtgaaccgg tgtatcaacc tggcagccca atgcaaaaag ctgaaaattc
     5941 ctatcaccac ttttatgatc gctaccgacc ctaacctgca aaagtttgtt caggaattta
     6001 cggagacaaa taatggaaaa gccttttttg catccctgga taaattagga gcatttattt
     6061 ttaaggattt tgagagtgga aaaagaaaaa cagtttattg aatgttgaac gaaaatcatt
     6121 aagatttatg aagatatatt acccaatcat aaaaatcatg aatacctgcg ttcccaatta
     6181 ttgaaaatat gaatatcaaa aagataaaga ctttaggcga actgaaagct tccggctatc
     6241 agccaaaaag catcaaggaa gagatacgcc agaatctaat tgcaaaactg caaaagaatg
     6301 aacccacttt ttcaggtata gtgggttatg aagacacggt gattcctgat gtggaaaggg
     6361 cattgctcag caagcacaat attttatttc tcggtctccg gggccaggcc aaaactagaa
     6421 tggcccggca gatgactgag ctattggatg aatatattcc ctgtgtggca ggcagcgata
     6481 tcaatgacga tccggttaac ccggtatcaa aatatgcgca ggaagaaatt gccacccatg
     6541 gtgacgaaac ggccattcac tggatacatc gcagtgaacg ttacggtgaa aaattagcga
     6601 ccccggatgt aagtgtagct gacctgatcg gtgatattga ccctatcaaa gcggctaacc
     6661 ttaaactcag ttttgctgac gaaagagtat tgcattatgg tatcatcccc agaagcaaca
     6721 gaggcatttt tgtaatcaat gaattacctg atttacaggc ccgtatacag gttgccttgt
     6781 ttaatatctt agaagaaggc gacgtgcaga taaggggatt taaacttcgt ttaccgcttg
     6841 atatcatgtt tgtatttacg gccaaccccg aagattatac caaccgcggt tccattgtaa
     6901 cacctttaaa agaccgtatt gaaagccaga tactaaccca ctatcccaag tcgctggaaa
     6961 atgcactgga gataaccgaa caggaagcag aaataagtgc tgatcagaaa gcaaaagtaa
     7021 aagtgagtga cctgatcaag cgtttgatag aacaggtagc ttttgaagcc cgtagcagcg
     7081 aattcgtgga taagaaaagc ggcgtgagtg caagacttac tatttctgcc tttgagaatg
     7141 cggtaagctc tgccgaacgc agagccatca tcaataacga aaaacaaaca caggtctgga
     7201 tgagtgacct ggttggcatc attccttcca tcacagggaa agtggaactt gtttacgaag
     7261 gtgaacagga aggtccttac caggtagcca tgaatctcgt tgacaaagcc atccgttctc
     7321 agtttgcaca atactttcct aatcccgata cattaaaaaa gagaagaaat accggcaaac
     7381 catcgcagga agaacaaaaa gatgataatc cttatcgccc tatcaccgga tggtttgata
     7441 aaggcaataa cctgaacctc tctttcaaca ccagtgataa agacaaaatg ttattattgt
     7501 ataaagtgga tggactgaat gccctggtaa aaaaatactt tccaaagtcg aatgaagaac
     7561 aggcagcctt actgatggaa ttcgttttac atggcctttc ctcctattca ttgatcagta
     7621 agaaaatatt tgagaataag atcgaattca aggacctggt aggaagtatg atgaacttca
     7681 gcgagaagga ttttgaattc gatgaagagg cttaattaac agctggtaag cagggacaaa
     7741 gaaaaatatt tcttacctgc ttatcagtat cccggtgcca ctaaataata ttgacctctc
     7801 tttccagtgt aatgccaaac ttctctttca cactttgtat tatttcttca ctgagattgt
     7861 atatctcagt gccggtcgca ttgccataat taaccagtac caatgcttgc tttgcatgac
     7921 atccggcatc acctttccgg tatcctttcc agccacattg ttcaattagc caaccggcgg
     7981 ccagttttac agtgccatca atattctcat gccctgccat attctcaaac ctggctttca
     8041 aggatacaaa ttgctcattt gtaacagacg gattcttaaa aaaactcccg gcattcccaa
     8101 tctctcccgg gttggggagt ttggaatttc ggatatttat tacagcagct gagattgctt
     8161 ttaaagaaag ttccctcact cccatccttt gcagttcctg ttcaatagcc ccgtaactag
     8221 tattgaaccg gggtttctta cgcagctgaa aagtaacatt caatattaca aactggtctt
     8281 taaatttttc tttgaacaca ctttcccggt agccaaattc acaatcactc cgggtaaacg
     8341 taactacctt cttgtccttt ttatgaaagg cttccaggtc ccagaacaca tcatcgatct
     8401 ccactccgta tgcaccaatg ttttgcatgg gtgaagcccc cacattgccg ggaatcaatg
     8461 atagattttc taccccagcc cagtttcgtt caatacaata caggacaaac tggtgccagt
     8521 tttcgcctgc acctgctttt acatatacat actcactgtc ttcgtgcaat tcaactatgc
     8581 ccctgatctc atttttcaga accagcccat cataatcttt ggtgagtaaa atattactgc
     8641 cgccaccgag aataaggttt gaggtttgag atttgaggtt taaagtttcc gcaagttcat
     8701 cagtgtcatt aaaggttgcg aaatatcttg cttttacatc aatcccgaat gtattatacg
     8761 gcttcagtga aatattttct tgcaacagca tactgcaaaa ttcatgtttt tttcgttata
     8821 aacagatgaa ctaacttcat aaccagttat taattgtctt ttggtataaa tcaaaaaata
     8881 tgaaaaaggt ctttttattg ctgttactcc ctgcattcat tatttcctgc aaaagtaaag
     8941 caccgaaaac tgagaataca actgaaactg ttgttgccac tcatcctgaa tggatcatgc
     9001 agggtaatat ttatgaagta aatgtccgcc aatatacttc tgaaggcaca tttaatgcct
     9061 ttgcccaaca cctcgaccgg ctgaaaaaca tgggcgtaca aaccctttgg ttcatgccag
     9121 tcaatcctat cagcaaagtg gatcgtaaag gcacactggg cagttattat gcagtagctg
     9181 actacactgc cattaatcct gaatatggca cgatggatga ctggaaaaaa ctcgttacta
     9241 ccattcacga aaaaggaatg aaggttttga tcgactgggt acctaaccat accggtgccg
     9301 atcacccctg gttaaccact caccctgatt tttttgtaaa agacagcaca ggtaaagctg
     9361 caattatgtc cgactggaca gatacccgtg tactggattt taaaaacaac gagatgcagg
     9421 acagtatgat tgctgcgatg aaattttggg taacccaaac aggcatagat ggctaccggg
     9481 tagatgtagc ctggaatgtc ccgggtgaat tctggaaaaa atgtatccct cagctaaaag
     9541 cagtaaaaaa tgatttgttc tttttagccg aaggagataa accgtactta atgatcagcg
     9601 gctttgacgc ctattatccc tgggaaatgt ttgtaatgat gacgaaagtg gcggctggcg
     9661 aaagacctgc ttttgcactc gacagtgtga aagcgaaata tgatagtgta tatccgaaag
     9721 aggctatccc tatgtacttt accagtaatc atgatgagaa cagctggaac aaatcagact
     9781 atggcacttt tcccggaata gtccatgctc cttttgctgt attcagccaa acgatggggg
     9841 gaaccgtacc ccttgtatac agcgggcagg aagaaccttt actgcgtcct ttgaaattct
     9901 ttgagaaaga ccccatagta tttggaaaat ttgaaagggc taaattttat aagagtctgc
     9961 atgatcttcg taaaaagaac cctgcccttg ctgctgatgc atctttcaaa aaagtaaacc
    10021 ttggcaatga cacaactatt tatgcatacg taagggaaaa aggaaatcat aaagtattgg
    10081 taatcctgaa cctgacaaaa aaagaacagg atataaaaat cactgataag agtttgcagc
    10141 gtttggctta taatattttc acgggtgtaa atgagcaatt aagtgagaaa agaaaaagtt
    10201 tatccccatg gagttatttt gtgtatgaat atcctgttgc cggtgcaaac taaataatcg
    10261 ggaaaaatcc attacgctat ttaaataaat gatcctttta tgagggcaac agttatcggt
    10321 gcaacggggc ttatcggcag tcacttgctg gaattactgc ttgctgatga caattttgat
    10381 tcagtgagga tactccttcg caggccattt gatcttactc atcccaaact ggagaagaaa
    10441 ctggttgact tttccgatat ggaaagtttc cggctggcac tggaggacag cgatgtgatt
    10501 ttctgtgcaa tcggtactac ccaaaagaaa gtaaaaggcg ataaatctgc ttaccgtaaa
    10561 gttgattttg atattgcggt gaattcagcc caactgggca agctatgcgg ctgtgaaaca
    10621 tttgtattag tatctgccgt tggcgctaac agtcaaagca ataatttttt acctgaaact
    10681 gaaaggtgaa acagaggaag ctgtgaaagc aacagggata aaatccattc acattgttcg
    10741 tccctcctta ttgctgggcg atagaaaaga atttcgtttg ggtgaaaaaa tggcgcaatg
    10801 gatgatgccg gtatttttgt ttttgattcc gaaaaaatac agaccaatac atgcaaagaa
    10861 ggtggctgag atgatgataa atgctgcaaa aataaccggc ccgaacagat gaagggaata
    10921 aatacattat tactgttgat aagcccggaa gaaccctata aagtgcaaag aaaagatcag
    10981 gaaaattacg aaacagcggc actggaagaa aaataaagct gcctccccat cctcttatca
    11041 ggtattattc cccgcaccag gaggttctgc ttgcttatat cttttaagca tccagccatt
    11101 aatcgcaaga caacacaata acggcaggaa gagcatgaca aaaaagttaa aataagaaac
    11161 cgtaataaca ggccggggca acacttctat aaaaacctgt tccccgatcg tattgcctga
    11221 aaacacaacc atctgaacta tgttccagcc gaaatgaata gctaccggca catacaatga
    11281 gaaagttttc gcatacccat aagccagtac caatcccata gcaccagaag taaaaaattc
    11341 gatagccatt tgaacagggt tcccccaaag ctcatgagag aaccagtggt acataccaaa
    11401 tgctgcagct gaaaagaaaa tagcttttac agcacccatt tttttaataa ggat
//
LOCUS       DAORTI010000114        11351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_5913_length_11351_cov_4.766732, whole genome shotgun sequence.
ACCESSION   DAORTI010000114 DAORTI010000000
VERSION     DAORTI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5913_length_11351_cov_4.766732"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..630)
                     /locus_tag="PLO70_12850"
     CDS             complement(106..630)
                     /locus_tag="PLO70_12850"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75401.1"
                     /translation="MKAIVFLIAFTSSASYPSFSQSREAGTDLGEIKQYMRNKDTNTT
                     SPLNKNIAAVRAVLQQYNSAIEKLDMTGTDNLFTNDSEIYESGLSEGNYTNFMNKHLK
                     PELKELKSFKFNNHNTDVTIIGNHAFATETYSCITVGAKHNTEVKRKGVTTSVLKKQK
                     GEWKIMNYHNSSTE"
     gene            complement(636..1205)
                     /locus_tag="PLO70_12855"
     CDS             complement(636..1205)
                     /locus_tag="PLO70_12855"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ75402.1"
                     /translation="MNTFLKSVLSAVILFFAITATAQKNEEVCYNPNLIKDTTKKSIK
                     SMAVAVTNGDSIKINYHSPGVRKRVIWGGLVPYDEVWVTGAHDATTLEIGKPFVVEGK
                     EIAAGKYAFFTIPGKKEWVVIINKNWEQHLASEYDEKDDVVRVKVKPKKNNHTERLQY
                     FVETAKGNDGKISMAWERIKIEFEIKFIK"
     gene            complement(1226..2422)
                     /locus_tag="PLO70_12860"
     CDS             complement(1226..2422)
                     /locus_tag="PLO70_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ75403.1"
                     /translation="MRYIIIIATAIFFFVSCKNKKEIPKDPDTYYTCSMDPQVVEYKP
                     GKCPICKMELTPVKKKNGEKKDELQLSDQQIQLGNIQTDTIHNGTIGDQLVLTATLNF
                     DQMKSSSVSSRVMGRIEKLYFKNLGDFVKKSSPLYDLYSEELNNSKQEYLLALDRKTS
                     FSGETVIDFDQLIQSAKNKLLLWGMSETQVAEMAKNKKATPTTTFYSSAGGYITQLDI
                     REGDYIMEGGTIVKLADLSSLWAEAQVYTSQLAEIDRNSIATVQLPDFDGKEIKGRIE
                     FINPEINPDTRINLIRVSIPNPGNQLKPGMPAYVLLKSPERKTLTLPINAVIRDGKGA
                     TVWIQTADKTFKSLMVQTGLESDDRIEIKSGLKEGDIVVITGAYLLHSEYVFKKGADP
                     MAGHNH"
     gene            complement(2425..3789)
                     /locus_tag="PLO70_12865"
     CDS             complement(2425..3789)
                     /locus_tag="PLO70_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ75404.1"
                     /translation="MKRIYFLTALIVMIVTACNNNKDPHAVHKTEVAKEMYTCPMPED
                     SVFSDKPGKCPKCGMDLVMMDNTEHDGHAAVEYTCPMHPEIIRDKPGTCPICGMDLVK
                     KETGGAKSGDAELEALLKPTNEFVVSSIPVTSIQKKEEEIEIDALGTIAYDTRQVGSI
                     SSRVTGRIEKLYVRYRYQKINKGQHILDIYSPELMTAQQNLLFLIKNDAENVTFIQAA
                     KEKLLLLGMSNEQLQQVIKAGRPSLTIAVYSNYSGHIHEAANGGSMNTGSGTMKDISL
                     ITEELSLKEGMYIQKGQSAFTVFNPDRAWAILSIYGDNQALVKVGNTVRLIPETAPNK
                     DFRASIDFIEPFYRKENKTLTARVYFNNSVLKIPIGSQVRATVFGNTKEAYWLPKEAV
                     LSLGMDKVVFQKTDGGFKAYKINTGIIHQKHIQVLSGLTEKDSVAANAQYLMDSESFI
                     KIKN"
     gene            complement(3801..5303)
                     /locus_tag="PLO70_12870"
     CDS             complement(3801..5303)
                     /locus_tag="PLO70_12870"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQZ75405.1"
                     /translation="MKRRNFIKISGLSAGTVLTGGAVAGFLASCSGCKKDYRMGLMTE
                     PVPVTEGDFSRLLSYPAQASANHTLTAQATVANIKGTNIPVLGYQSNSLLGPSFRINR
                     GDAINVLLQNNLSEHTNIHWHGLKIPALMDGHPDQLAGAGGTFRYQFTVNQRAGLSWY
                     HPHPHEMTGKQVFQGLAGMFIINDTEEAALSLPSGQYELPLVIQDKRISSNGITYNPS
                     MEEVMAGYMGESIIVNGIYSSYTEVATRCYRLRILNGSNARVYNLALSNNADMIIIGN
                     DGGLLKNPVAVKEILVAPGERLDVLVNFSSSTIGTEIYLISKEFGNGGNAQGKQGFKI
                     MKFKVAVSSAITFTVPANLSAITAIAAASAVRTRVFEISNAMEHHGVPMNNGMQMRHR
                     INGKLFDSSRIDETVAANTNEIWVFDNSNGDEPHPMHLHGVFFQVLQRSGGRDNLIAS
                     ENGWKDTVLVMPGETVKVLVPFENNTGKFVFHCHNLEHEDDGMMLQYQLS"
     gene            complement(5315..6568)
                     /locus_tag="PLO70_12875"
     CDS             complement(5315..6568)
                     /locus_tag="PLO70_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ75406.1"
                     /translation="MLKNKLLPALFALLAASGSKSQTLKLDAIIDSIKASHPVVKMYD
                     NEIRSMDEAAKGARSWMPPQFGIGQFMTPYDARLWHREGDIPGMGSVMVSGEQMFANK
                     RKLDADERYMKAMSSVEKEKKNATLNELTNDAKQFYYEWIILKKKLLVLTENEKILDF
                     MIKNAEIRYRNGLEKISAYYKAKAALGDVKNMQLMFENDIKEKRIRINALMGRDAMHY
                     FDIDTTYQLTDFANIVFDSTLFYNSRSDLKSLDREINLTTLKQETEKQSLKPQFGIRY
                     ENMIGFGGQPLQYTIMGMVRIPMAKWSSKMNKANIESLKWKANAIQSQKEMMVNEYSG
                     MAYSMRNEMELKKKQVKLYEENIIPALKNNYKTMQLGYEQNTEELFMLYDAWEILNMK
                     QLEYIELLNQALRLQVTIERIIENK"
     gene            complement(6555..8480)
                     /locus_tag="PLO70_12880"
     CDS             complement(6555..8480)
                     /locus_tag="PLO70_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQZ75407.1"
                     /translation="MAKWFKRNTDPLTSEEKMDIIEKSSKQVGPGVFYSTIIVVASFL
                     PVFLLTGMEGKLFHPLAWTKTFILLIDAFLAITLTPVLISFFLNGRLKPESANPITRT
                     LERIYTPILKWCLKWRKTTIGINLAALAVGIIMMTRLGSEFMPPLDEGSLLFMPVTLP
                     DVSNSEAKRLLQVQDKLIRTVPEVYHVLGKAGRANTATDNSPISMIETIILLKPKSEW
                     RKGMTKDGIINELNAKMQIPGVTNGWTQPIINRINMLSTGIRTDVGLKVYGQNIDSIY
                     AFAQTIKKQLEGIDGIKDLYVEPITGGKYIDIAIKREEIGRYGLSVDDVNLVIESALG
                     GMKLSTTIEGRQRFSVNARYGQDFRNNLESLKRLQVQTMNFGPIPLEAVADMKLSDGP
                     PMINSENAMLRGTVLFNVRERDLGSTVKEAQQKLNKMITKLPKGYFLEWSGQWENQIR
                     ANRTLKTIMPIVLIIIFMVLYFTYHSFKEAAVTMLTVPFALIGGVFMVYFYGINLSVA
                     VAVGFIALFGMAIETAMLMTIYLNEAMHNMVAKHGNSKATLTPAVIMEFVSEGAAKRL
                     RPKLMTVSVGLFGLIPILWATGVGSDIMRPITIPLIGGTITSTIYVLLITPVIFEMIK
                     ERELKRKGKIELIDVKE"
     gene            complement(8483..9772)
                     /locus_tag="PLO70_12885"
     CDS             complement(8483..9772)
                     /locus_tag="PLO70_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQZ75408.1"
                     /translation="MVRKLIELALRNRVIVLLIAGGLFAYGIYSIKKNPIDAIPDLSE
                     NQVIVFTEWMGRSPQVIEDQVTYPLVSNLQGIPKIKNIRGSSMFGMSFVYVIFEDNVD
                     IYWARTRVLERLNFAQRLLPQGVTPSLGPDGTGVGHIYWYHLDAPKMDLGEQRALQDW
                     YIKFALQTVKGVAEVASFGGFEKQYQLVVDPVKLQYYNISLMDVMNKVKANNNDVGGR
                     KFEMADMAYIIRGLGYIKSKEDVENIALSNYNGIPVRVKDIGSVQMGGDLRLGIFDMD
                     GKGEVVGGIVVMRYNENANKVIEAIKEKMKEVEKGLPEGVTFKTSYDRSTLIQEAIAS
                     VKGTLIEEMIAVSLVVLIFLFHWRSAVIILIQLPISVAAGFIFLEMFGISSNIMSLTG
                     IALAIGVVVDDGIVMVENAYRHISEAQTEKSKTTNKT"
     gene            complement(9789..10421)
                     /locus_tag="PLO70_12890"
     CDS             complement(9789..10421)
                     /locus_tag="PLO70_12890"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HQZ75409.1"
                     /translation="MKRIFSSVVTISTIFFTACNSGGSKNEHEGHDMKNMNKDTTQHA
                     VTPDDKEVKAVTVAYTNVDAKATASIKEIVDHYLNIKNALANDNGSEAASGAKAMENA
                     ISKLDKSLLTAEQKAAYDKNEEEMKEHAEHIAKNGDKIEHQRSHFVMMSEVVYDLVKN
                     FGAGRPLYHDHCPMARNNQGAMWISEMKEVKNPYFGAKMLTCGTIEEVIK"
     gene            complement(10444..11229)
                     /locus_tag="PLO70_12895"
     CDS             complement(10444..11229)
                     /locus_tag="PLO70_12895"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HQZ75410.1"
                     /translation="MKSFTMLMMAALTILSVSVFAQDSTKHKMKVPKQKTEKTKYSCP
                     MHPNEVNDKAGKCSQCGMDLALSPKEKMKMEVMKTYTCPMHPETVSDKPGKCPQCGTI
                     MNRSPKEKMKMEVMKIYTCPMHPDENSNKSGKCSKCGMELKEKGKTTQSYTCPMHPEV
                     TSDKPGKCSKCGMNLNLSPKEKMKMEVMKTFTCPMHPEVTSDKPGKCTKCGMALKEKE
                     QQAIAYSCPMKCEGDKTYDKPGKCPKCGMDLKEKKDDHSNHQH"
BASE COUNT         3029 a         2479 c         1975 g         3868 t
ORIGIN      
        1 acatttagaa catttttcag gtttgctact ggtaacttct ggatgcatgg ggcaagtata
       61 gttttgctct tgatgcttat gttccataac aaaatattta gagacttatt cagttgatga
      121 attatgataa ttcataatct tccattcgcc cttttgcttc ttcaaaacgg atgtagtaac
      181 acctttacgt tttacttcag tattgtgttt tgcaccaaca gtgatgcaag agtaggtttc
      241 agtagcaaaa gcatgattgc caataatagt gacatcggtg ttgtggttat taaatttgaa
      301 cgatttaagt tcttttagtt caggctttaa atgcttattc ataaaattag tgtaattccc
      361 ttcgcttaaa ccggattcat aaatttcaga atcattggta aacaaattat cagtacctgt
      421 catatccaat ttctctatgg cactattgta ctgctgtaaa acagctctga cagccgcaat
      481 atttttattt aaaggagaag ttgtgttcgt gtccttattc ctcatgtatt gctttatttc
      541 acccaaatca gtaccggcct ccctgctttg ggaaaaggat ggataggaag cagaggaagt
      601 aaatgcaatc aggaatacaa tcgctttcat gataattatt ttatgaattt aatctcaaat
      661 tcaattttta ttctctccca ggccattgaa atcttcccat cattgccttt agctgtttct
      721 acaaaatatt gcaacctttc agtgtggtta tttttcttag gcttaacttt tactctcact
      781 acatcatctt tttcatcata ctcagatgca aggtgctgtt cccaattttt attgataata
      841 acaacccatt ctttctttcc gggaatagtg aaaaaagcat acttgccggc cgcaatttct
      901 ttgccctcaa caacaaaggg tttaccgatt tcaagggtag tagcatcatg agcacctgtt
      961 acccacactt catcataagg aacaaggccg ccccatatta ctcttttacg aacaccggga
     1021 gaatgatagt tgatttttat gctgtcgcca ttggtaactg caacagccat tgatttaata
     1081 ctctttttgg tagtatcttt tataaggttt ggattgtagc agacctcttc atttttttgt
     1141 gctgttgcgg tgatagcaaa gaaaagtatt actgccgata gaactgactt caaaaatgta
     1201 ttcattttgt tagattgata aattattaat gattatgtcc ggccatcggg tcggctcctt
     1261 ttttaaatac atactcgctg tgcaataaat aagctcctgt aataaccact atatcgcctt
     1321 ccttcaaacc tgattttatt tctatcctgt catcgctttc caaacctgtt tgtaccatca
     1381 ggcttttaaa tgttttgtct gctgtttgta tccatactgt tgctcccttt ccatcacgga
     1441 tgactgcatt aattggaagt gtcaacgttt ttcgctccgg acttttcagg agtacataag
     1501 caggcatacc gggttttaac tgattgccag ggttgggaat tgatacacgg ataagattga
     1561 ttcttgtatc cggattgatt tcagggttta taaattcaat tcttcctttt atttccttcc
     1621 catcaaaatc agggagttgt acggtggcta tactgttcct gtctatctcc gccagttgtg
     1681 aagtataaac ctgcgcctct gcccaaaggc ttgacaagtc ggccaatttt acaatagtgc
     1741 ctccttccat tatgtaatca ccttctctta tatccaactg cgtaatgtaa ccaccggcag
     1801 aactgtaaaa tgtagtagtg ggtgttgctt ttttgttttt tgccatttcc gctacctgag
     1861 tttcactcat gccccacaag agcaatttat tttttgcact ttgaatcaat tggtcaaagt
     1921 caataaccgt ttcaccagaa aaagatgttt tcctatccaa tgccaataga tattcctgtt
     1981 ttgaattgtt cagttcttca ctgtataaat catatagtgg tgagcttttc tttacaaaat
     2041 cacccaggtt tttgaaatac agcttttcta tcctacccat cactcttgaa ctcacagaag
     2101 atgatttcat ctggtcgaaa ttcaatgtcg ctgttaatac taactggtcg ccaattgtac
     2161 cgttatggat tgtatcagtt tgtatatttc ccaactgtat ctgctggtcg cttagttgca
     2221 gttcatcttt cttttcaccg ttcttttttt tcacaggagt caattccatt ttgcaaatag
     2281 ggcatttacc cggtttgtat tccactacct gcgggtccat agaacaagtg taataagtgt
     2341 cagggtcctt gggtatttct tttttgtttt tacaggaaac aaaaaagaaa atagccgttg
     2401 ctattataat gatgtaacgc ataatcaatt tttaattttg ataaaactct cactgtccat
     2461 taaatactgt gcattggcag ccaccgaatc cttttctgtt aacccactta atacctgtat
     2521 gtgtttttgg tggatgatac cggtattgat tttatatgct ttaaaaccgc catcagtttt
     2581 ttgaaatacc actttatcca tacccaacga cagcaccgct tcttttggta gccagtaagc
     2641 ctcttttgta ttgccgaaaa cggttgccct tacctggctg ccgataggaa ttttcaatac
     2701 actgttattg aaataaactc ttgccgttaa tgttttattc tctttacgat aaaagggttc
     2761 aataaaatca atagaagccc tgaaatcttt attaggtgct gtttcaggaa taagccttac
     2821 ggtattacca acttttacca atgcctgatt atctccataa atacttagta ttgcccatgc
     2881 cctgtctgga ttaaagacgg taaaagcaga ctgccctttc tgtatataca taccttcttt
     2941 caatgaaagc tcttctgtaa tcagtgaaat atctttcatc gtaccggacc ctgtattcat
     3001 actaccgcca ttagctgctt catgaatatg gccactgtaa ttactatata ccgcaatggt
     3061 caatgagggt cttccagctt taataacctg ctgcaactgt tcattactca taccaagcaa
     3121 taacaatttt tctttggcag cctgaataaa tgttacattc tctgcatcat tcttaatcag
     3181 gaacaagagg ttttgctggg ctgtcatcaa ttcggggctg taaatgtcca atatgtgctg
     3241 gcccttattt attttctggt aacgataccg tacatacaac ttttcaattc ttccagtcac
     3301 tcttgatgaa atactcccca cctgcctcgt atcataggca atagtgccca atgcatcaat
     3361 ttctatctct tcttcttttt tctgtattga agtaactgga attgatgaaa caacaaattc
     3421 attagttggc tttaataatg cttccagttc tgcatcacca gattttgcac ctcctgtttc
     3481 cttttttacc aagtccattc cgcagattgg gcaagtaccg ggtttgtccc ggatgatttc
     3541 gggatgcatg gggcaggtgt attcaacggc tgcatgacca tcatgttctg tgttatccat
     3601 catcactaag tccattccac acttcgggca tttaccaggc ttgtcactga atacagaatc
     3661 ttccggcatc gggcaggtgt acatttcctt tgctacttcc gttttgtgta ctgcatgagg
     3721 gtctttgtta ttgttacaag cagtaacaat catcacaatc aaagctgtta aaaaataaat
     3781 tcgcttcata ctatttttgc ttaactcaat tgatactgta acatcatacc atcatcttca
     3841 tgttccaggt tgtgacaatg gaacacaaac ttgcccgtat tattttcaaa aggaaccagc
     3901 actttaactg tttcacccgg cataactaat acggtgtcct tccatccatt ctcagatgca
     3961 atcaggttgt cccttccgcc actgcgttgc aatacctgga agaacacacc atgcaggtgc
     4021 attggatgcg gctcatcacc attgctgtta tcaaataccc atatttcatt tgtattggct
     4081 gctaccgttt catcaatccg actactgtcg aataattttc catttatgcg gtgacgcatt
     4141 tgcattccat tattcattgg cactccgtga tgctccattg cattggagat ttcaaatact
     4201 cttgtcctta ctgctgatgc tgctgctata gcagtaatgg cagaaaggtt tgcagggaca
     4261 gtaaatgtaa tggcagaaga tactgctact ttaaatttca tgattttaaa accctgcttg
     4321 ccctgtgcat taccaccgtt gccaaactct ttgcttatta aataaatttc agttccgatt
     4381 gtggaactgc taaagtttac aagcacatcc aatctttctc cgggggcaac caggatttct
     4441 tttactgcaa caggattttt taataagccg ccatcgttac cgataattat catgtcagca
     4501 ttgttactca atgcaagatt gtaaaccctt gcattggaac cgttgagaat acgaagacgg
     4561 taacagcggg tagcgacttc tgtataagat gaataaatac cgtttacaat aattgattcg
     4621 cccatgtaac cggccatcac ttcctccatt gatggattat aagtaatgcc gtttgatgaa
     4681 atacgtttat cctgtattac caatggcaat tcatattggc cggatggaag gcttaacgcc
     4741 gcctcttcag tatcattaat aataaacatt ccggcaagac cctggaacac ctgtttacct
     4801 gtcatttcat gcggatgcgg atgataccag cttaacccag ctctttgatt tacggtgaac
     4861 tggtaacgaa atgtaccccc tgcaccagct aactggtctg gatggccatc catcaaagca
     4921 ggaatcttta aaccatgcca gtggatgttc gtatgttctg ataaattgtt ctgtaataaa
     4981 acgtttattg catcgcctct gttaatcctg aaagaaggac ccaacaggct attggactga
     5041 tagcctaata cgggaatatt tgttccttta atgtttgcta cagtagcctg tgccgtcaat
     5101 gtatggttag cacttgcctg agccggataa gaaagtaatc ttgaaaaatc tccttccgtt
     5161 accggtacag gttctgtcat caaacccatg cgatagtctt ttttacaacc actgcaactg
     5221 gcaagaaaac ctgcaacagc accacctgtt aatactgttc cggcactaag cccggatatc
     5281 tttataaaat ttctcctttt catatagttg ggttttattt attttcaata atacgttcga
     5341 tagttacctg caatcgcaaa gcctgattta gtaattcaat atattcaagt tgcttcatgt
     5401 ttaatatttc ccatgcatca taaagcataa agagctcctc tgtgttctgc tcatatccca
     5461 actgcattgt tttatagttg ttcttcaatg ccgggataat attttcttca tagagtttaa
     5521 cctgtttttt ctttagttcc atttcattcc gcatactata tgccatgccg ctgtactcat
     5581 tcaccatcat ctctttttga gattgaatag catttgcctt ccatttcagg ctttcgatat
     5641 tggctttatt catctttgat gaccatttag ccatcggtat tctaaccatg cccataatag
     5701 tatattgtaa tggctggccc ccaaagccaa tcatattctc ataacgtatc ccaaattgtg
     5761 gtttcaggct ttgcttttct gtttcctgtt tcagtgttgt aagattaatc tcccggtcta
     5821 atgattttaa atcactccgg ctgttataaa agagtgtact gtcaaaaaca atatttgcga
     5881 agtcagtaag ctggtaagta gtatcaatgt caaaatagtg cattgcatcc ctgcccatca
     5941 gggcattgat gcggatacgt ttttctttta tgtcgttttc aaacattaac tgcatgttct
     6001 ttacatcccc caatgcagct ttagctttgt aataagcact tattttttcc aacccgtttc
     6061 tgtaccgtat ttctgcattt tttatcatga agtccagaat cttttcattt tctgtgagta
     6121 ccagtaactt tttcttcaga attatccatt cataataaaa ttgttttgca tcattagtca
     6181 attcattcag tgttgcgttc tttttttctt tttccactga agacatggcc ttcatgtatc
     6241 gttcatctgc atccagtttc cttttattgg caaacatctg ctcgcctgaa accattacgg
     6301 aacccatgcc cggtatatcg ccttccctat gccagagcct tgcatcatac ggagtcatga
     6361 actgtccgat accaaattgt ggtggcatcc agcttcttgc acccttagct gcttcatcca
     6421 ttgaccggat ttcattgtca tacatcttca ccaccgggtg agacgctttg atgctgtcaa
     6481 ttatagcatc gagttttaat gtttgcgact tactgccgga agcagcaagc agggcaaaca
     6541 atgccgggag tagtttattc tttaacatct atcaattcaa ttttgccttt acgttttaat
     6601 tctctttcct ttatcatttc aaatattact ggtgtgataa gtaatacata aatagttgaa
     6661 gtgatagtac cgccaatcaa tggaatggta ataggccgca tgatatcgct gccaacaccg
     6721 gttgcccaca gaataggtat caaaccaaaa agaccaacag ataccgtcat taattttggt
     6781 ctcaaccttt tagccgcacc ttcacttaca aattccatga ttacggctgg tgttagtgtt
     6841 gctttggagt taccatgttt tgctaccatg ttatgcatag cctcattcaa ataaatggtc
     6901 atcagcatgg ctgtttctat tgccattcca aataaggcga tgaaaccaac tgcaacagct
     6961 actgataaat tgataccata aaaataaacc atgaacacac ctccaatcaa tgcaaaggga
     7021 acagtaagca tggttaccgc tgcctcttta aaagaatgat aagtaaaata cagcaccata
     7081 aaaatgatga tgagaacaat tggcattatg gtctttaatg tcctgtttgc ccttatctgg
     7141 ttttcccatt gtccgctcca ctctaagaaa tagcctttgg gcaatttggt tatcatcttg
     7201 tttagtttct gctgtgcttc ttttactgta ctgcctaaat ctctttcccg tacattaaat
     7261 aataccgttc cccgcagcat tgcgttttca gaattaatca tcggggggcc atcactcaat
     7321 ttcatatcag ccaccgcttc caggggaatg ggaccgaagt tcatcgtttg cacctgcaat
     7381 ctttttagcg attcaaggtt gttacggaaa tcctgcccat atctggcatt tacagaaaaa
     7441 cgctgacggc cttctatcgt agtagaaagt ttcatgccac ccaatgcact ttcaatgaca
     7501 aggttaacat catccacact taaaccatac ctgcctattt cctctctttt tattgcaatg
     7561 tctatgtatt tacccccggt gataggttct acatacaaat cttttatacc atcaattcct
     7621 tccagttgtt ttttgatagt ttgtgcaaaa gcataaatac tatcaatgtt ttgtccatat
     7681 acttttaacc ctacatcagt ccggataccg gtagaaagca tattaatacg gttgataata
     7741 ggttgtgtcc atccgttggt aacacccggt atctgcattt ttgcattcag ttcattgatg
     7801 atgccatctt tggtcattcc tttacgccat tcacttttgg gcttcagtag tatgatggtt
     7861 tcaatcatgc tgataggcga attatccgta gccgtatttg ccctgcctgc tttccccagc
     7921 acatgataaa cttcgggaac agtacggatt agcttgtcct gcacttgtaa taatcttttt
     7981 gcttctgaat tggatacgtc cggtaaagta actggcataa aaagtaatga accttcgtcc
     8041 agcggtggca taaattcaga acccaggcgg gtcatcataa ttataccaac agctaatgcg
     8101 gcaaggttga taccgatagt tgttttgcgc catttcaaac accatttaag aatgggtgta
     8161 taaattcttt ctaatgttct ggtaatcggg ttagcacttt caggcttcag tcttccattt
     8221 agaaaaaatg aaatcagtac aggagtcaat gtaattgcta agaaagcatc tatcaacaga
     8281 ataaatgttt ttgtccatgc aagcggatga aaaagttttc cttccatacc agttaacaaa
     8341 aacaccggaa ggaaagaagc aactacaatg atggtggaat agaaaacacc tggccccacc
     8401 tgtttagaag acttttcaat gatgtccatc ttttcctcag atgtcaacgg gtctgtattt
     8461 cgtttaaacc actttgccat gtttatgttt tattagttgt ctttgatttt tctgtttggg
     8521 cttcactgat atgccggtaa gcattttcca ccattacaat tccatcatct acaaccacac
     8581 caattgccag ggcaatacct gtcaatgaca taatgttgga agagatgcca aacatttcaa
     8641 ggaatataaa accagccgct acggaaatgg gtaattgaat taaaatgata acggcacttc
     8701 gccagtgaaa taagaaaatg agtacgacaa gcgatacagc aatcatttct tctattaatg
     8761 taccttttac tgatgcaatg gcttcctgta tcaaagtgct tctgtcgtat gaagttttaa
     8821 aagttacccc ttcgggcaaa cctttttcca cttctttcat tttttcctta atggcttcta
     8881 ttactttatt ggcattttca ttgtaccgca ttactacaat gccgcctacc acttcgcctt
     8941 taccatccat atcaaatatg ccgaggcgta aatcgccacc catttgcact gagccaatat
     9001 cttttactct tacaggaatg ccattgtaat tgctcaatgc aatattctct acatcttcct
     9061 tgcttttgat ataccctaag ccccggatga tataagccat atctgccatt tcaaactttc
     9121 taccccctac atcattatta ttggctttta ctttattcat cacatccatc agggagatat
     9181 tgtaatactg caattttacc gggtcaacta ctaactggta ctgtttttca aaaccaccaa
     9241 aggaagctac ttcagctact ccctttacgg tttgcaaggc aaattttata taccaatcct
     9301 gtaaagccct ttgctctccc aaatccattt ttggtgcatc aaggtgatac cagtaaatat
     9361 gaccaacacc tgttccatca gggccaagtg aaggtgttac tccctgcggc aataatcgtt
     9421 gagcaaaatt caatcgttcc aatacccttg ttcttgccca gtagatgtca acattatctt
     9481 caaaaatcac atatacaaaa ctcatcccga acatggatga gccacggata tttttaatct
     9541 tcggaatccc ctgtagattg ctcaccaatg gataagtaac ctggtcttct attacctgtg
     9601 gactgcgacc catccattct gtaaaaacaa tcacctggtt ctcactcaaa tcaggaatag
     9661 catcaattgg gtttttctta atggagtaga tgccatacgc aaacaggccg cctgcgatga
     9721 gcaagacgat aaccctgttg cgtaaagcca gttcaattaa tttgcgaacc atgctgatgt
     9781 tttaattatt acttaatcac ttcttcaatg gtaccacagg taagcatttt tgcaccgaag
     9841 taaggattct ttacttcttt catttcacta atccacatgg ctccctggtt atttcttgcc
     9901 atcggacaat ggtcatgata cagcggacgg cctgcgccaa aattctttac caaatcatac
     9961 accacttcac tcatcatcac aaagtgggaa cgctgatgct ctattttatc accattcttt
    10021 gcaatgtgtt ctgcatgttc tttcatttct tcttcatttt tatcatacgc tgctttttgc
    10081 tcagccgtta gcaatgattt atcgagtttg ctgattgcat tttccattgc tttagcacca
    10141 ctggcggctt cgctgccatt atcattagcc agtgcatttt ttatattcag gtaatggtca
    10201 actatttcct taatggaagc ggttgctttt gcatccacat tggtataagc aacagtaaca
    10261 gctttcactt ctttatcatc tggggtgact gcatgttgag tggtgtcttt attcatgttc
    10321 ttcatatcat ggccttcatg ttcattcttg cttccgccac tgttacaggc agtaaaaaag
    10381 atggtactga tagttactac actagagaat atccttttca tatttgtttg atttttgttg
    10441 tggttaatgt tgatggttag aatggtcatc tttcttttcc tttaaatcca ttccgcattt
    10501 agggcatttg ccgggcttat cataggtttt atcgccttca catttcatag ggcaggagta
    10561 agcaattgcc tgttgctctt tttctttcag ggccatccca cattttgtac acttgccagg
    10621 tttatcgctt gttacttcag gatgcatagg gcatgtgaag gttttcatca cttccatttt
    10681 cattttttct ttgggtgaaa gatttaaatt cattccacat ttggagcatt tgcccggctt
    10741 atcgctggta acttccgggt gcatcgggca agtataagat tgagttgttt ttcccttttc
    10801 tttcagttcc atcccgcatt tagaacactt gccggatttg ttgctgtttt catcagggtg
    10861 catgggacag gtgtagattt tcattacctc cattttcatt ttttcctttg gagaacggtt
    10921 cattatagta ccacattggg gacacttacc gggcttgtcg cttacagttt ccggatgcat
    10981 ggggcaggta taggttttca ttacctccat cttcatcttt tcttttggag aaagagccag
    11041 gtccattccg cattgagagc atttaccagc cttatcattc acctcattgg ggtgcatcgg
    11101 gcaactgtat ttagtttttt ctgttttttg tttgggaact ttcattttgt gctttgtact
    11161 gtcttgtgca aaaacagaaa cagacaagat ggttaaagct gccatcatca gcattgtaaa
    11221 tgatttcatt ttatttgatt taatgtacag tgaagaaata caagaatatg gcatagagaa
    11281 atgccaatag cccgacaata aaattgcctg gctcataaaa gaaaataatc tttaaataag
    11341 gaaagtacaa t
//
LOCUS       DAORTI010000115        11034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_6151_length_11034_cov_9.802350, whole genome shotgun sequence.
ACCESSION   DAORTI010000115 DAORTI010000000
VERSION     DAORTI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11034
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6151_length_11034_cov_9.802350"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLO70_12900"
     CDS             <1..377
                     /locus_tag="PLO70_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQZ75411.1"
                     /translation="VNQSVFRTIVLATYNNTCCITSINNTNLLIASHIVPWSKDQKNR
                     LNPTNGLCLNALHDKAFDKGLITISAKDYTIRVSSKLKGEKNLQFIELNFIEYEGKKI
                     NLPDKFLPSKEQLKVHNESFIS"
     gene            complement(378..1532)
                     /locus_tag="PLO70_12905"
     CDS             complement(378..1532)
                     /locus_tag="PLO70_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75412.1"
                     /translation="MKQVISAILYLLISFNCFCQSNPEATILIVACKSGILVVDGNEI
                     GSVEADDAKKLTLSYGEHYLQLKNTTGKYNFTINVDESTKGIIKLGCDVESKIEKIRV
                     LDKEVALSGALGNDLEKNVIGFDKDDEVTINASLLNKKGLFTVSLFEYSTGREIYKKE
                     RIISIVNDKITIPTKGVYYFLVYQEGLFSKSAKLTVDRNPSKNGNPNFNTAVKIVYDT
                     TFTEVLNTVTRVYSTTNLEHSSRTVIKINLPSNTTYWAYWIGVGQESRTKYKTFAGTL
                     SGVGTLLSANPLVLFGLKLIPALPMLNTPSTITYKFTDTKNAQLFINGLAYSTYNFKY
                     ADNVSTDYSLIEQNVQDLVLTMKNSSTIDGQDVDIKVVAFIVNAKWVLHE"
     gene            1905..3206
                     /locus_tag="PLO70_12910"
     CDS             1905..3206
                     /locus_tag="PLO70_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQZ75413.1"
                     /translation="MLNNFFRLLIVLCCAGGTAHAQLTKETVQQHIRELLQTVTDNYV
                     YPAIAKKAKQYVLTLQEKGAYDTIQKGERLAAALTRDLQAITHDLHLRVFYSPTVLPK
                     QENSNGPSPEEIEGFKQMLKGANYGIKEKSVLEGNIGYLNITMFAPLDFCADSLTAAV
                     QAVINTDALIIDLRECGGSMDPDAIPFFSGYFFDRPTHLNDLYFRNTGDTTQYWSYGW
                     VPGKKYGNKPIYILTSGRTFSGAEEIAYDYKNLKRAILVGEQTKGGANPGGDMRVNDH
                     FGVFVPNGCAINPITKTNWEGVGVTPDTVISAMKALHKAQLMAVQHLWQQANTPEKKK
                     SLQEALTAIQKAAPVYKQVQFTLKGFEKAKEVFVAGSFNGWTPEQTPMLRKGKEWVAV
                     AEAPVGKLAYKFFVDGEWILDPAHTQTESDGRYTNSVKQVQ"
     gene            3431..4516
                     /locus_tag="PLO70_12915"
     CDS             3431..4516
                     /locus_tag="PLO70_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQZ75414.1"
                     /translation="MSQIKPAAIELLPLKQEFETKAVLRKTALAHQALAELKGVVTSI
                     PNQHILTETLTLREARESSAVENIISTFAEVYQSSLFSNLFASAAAKEVHLYARALKK
                     GYQLVKQHGLLTGNIILQIQEQVEGNSAGFRKLPGTKLLNDATGELVYLPPQDHETIV
                     SLMNNLEKFINDDTMMDADPLVKMAIIHHQFESIHPFYDGNGRTGRIINILFLIQKNL
                     LDIPVLYLSRYIIKHKNDYYRLLQAVRQTGKWEEWILYMLDGIEQTARESIILIGHIK
                     NLMLEYKQTLRTKLPKLYSQDLLNNLFKYPYTKIEYLEKELDISRSTAIRYLKELEKL
                     HLVSKQKIGRDNFYINQPLFNLLAANK"
     gene            complement(4520..6433)
                     /locus_tag="PLO70_12920"
     CDS             complement(4520..6433)
                     /locus_tag="PLO70_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQZ75415.1"
                     /translation="MATANRHLSETKEKGSAKAKISKESLRQALILFKYLKPHRGKFI
                     GGLIFIALSAFTTSLFPLFLGKMIDAASPGSEVPGMTTSQFDFGLKNIHWSLNTTLLL
                     IFCQLTLQTIFSFMRVYLLTSAGERSLADMRKDVYSKLLTMPMSFFTEKRVGELSNRI
                     SSDLSQIQDAISFTLAEFLRGIFTLIIGLFFIFWISAKLALVMLAVVPVIAILAVVFG
                     VRIRKMARRAQDQLADSGTIVQETFHGISIVKAFTSEIHEIGRYVKSIQSVVHTAISN
                     ARYRGAFISFMIFSVFGTIAFVMWVGAGMIQSGELTMGALTMFVIFSMFVGGTFAGFA
                     DMFSQLQKTLGATQSIREILRSKGEAVELDDIVVEPQYVLKGNVRFEQVAFSYPSRKD
                     VPVLRNLTLTAKDGEQIAIVGPSGAGKSTIASLLLHFYEPDSGTVYFDERPAKEFPLS
                     QLRRQMAFVPQEVILFGGSIRENIAYGKPHATEEEIIAAARSANAHEFIQRFPEQYDT
                     IVGERGIKLSGGQRQRIAIARAILKDPAILILDEATSSLDSESEQLVQEALDNLMKGR
                     TSFVIAHRLSTIRNANKIVLIDKGIVSEAGTHTELMAHNGLYRKLNEMQFEFGVVTQS
                     PAVNPDSYTEDEK"
     gene            complement(6614..>9089)
                     /locus_tag="PLO70_12925"
     CDS             complement(6614..>9089)
                     /locus_tag="PLO70_12925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HQZ75416.1"
                     /translation="TTEGKILLDLQHRDEVLESSLEIISPYEIKGMRRSKSWAQNQVV
                     YFYLKFDQPVKNYGIASKDEPVEPTDIKTASINGKNIKAYFAFAIAGNKTVQVKVGIS
                     GVSTENAKLNLDTELPDWNFNKVKDVAEAAWNKELSKIAISGGTPDQQVAFYTALYHT
                     MLAPNIYTDVNGEYRGTDLKVHNAEGFTNYTVFSLWDTHRALHPLHSIINRQRTLDWI
                     NTFLVQYQHGGMMPVWELSGNETFCMIGYHAVPVIVDAYKNGIRDFDTKLALKAMTDY
                     AESNRYGLPAYMKQGFISNEDDHESASKTVEYAYDDWCIAQFAQWTGNDEVYKKYLNR
                     SLQYRNLFDPRTRHIRGKVQGFWYQPFDATEVNNFFTEGNAWHYSFTAQQDIDGLKKL
                     HGGNEAFAKKLEELFITNTKLSGREQADVTGLIGQYAHGNEPSHHMAYLFNYAGKPWR
                     TQELVHQICTEFYPNNPDGLIGNEDCGQMSAWFVLSAMGFYPVTPGSGEYALGTTLFD
                     EVILHLENGKSFIIKAKNRKKENFYVEDVLLNDKKHAATFIQHSDIANGGELLFSMNN
                     KPGKTRGIAEKDMPHSVVNDDQFVAVPFFDMESYKFKTTAAVQLKHIDAAADSYYSAE
                     QEDGSSGEFIRYTKPFTIDKTIRVTIYAEKNGIKSPVTYRKFYKIPAEISITVLSEVH
                     PMYTAGGKEALIDGITGTENWKTGDWQSYYAKDFEAIIDLQKEKSLQYVGVHVLQDVS
                     PWIVYPKEVIFERSNDGKNWQPLTIVKNNIGTDIKGPVHQQIGAAVMAKARYIKVKAI
                     SGGSLPAWHESAGYPSHLFIDEVLIR"
     assembly_gap    9090..9189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9556..>11034)
                     /locus_tag="PLO70_12930"
     CDS             complement(9556..>11034)
                     /locus_tag="PLO70_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75417.1"
                     /translation="NAITGNVTIANDASYSGGYYTYLRSNIINGNTSIANNGSNVLLD
                     ANQAGTGNKYLGNVTYIKTGAGISVGAGDVNEIGGNLTLNSTDGIGLGKIKLNGSTSG
                     IIEQQSTQPVIIAELSIDKTGGGTITLNDTVSISTSTSFINGIINSSAGKELIFLNNA
                     NYSGASNISFVNGPCKKIGNDAFIFPVGKENKIAPISIAAPAFTTDAFRAEYFMSVPH
                     DSGYDSTQKGPGIDHISLREYWLLNRTEGVSATAVTLSWEASRSGGINNLGDLRVARW
                     NGSLWTDEGNGGTTGNNTAGTILTAGVVTDFSPFTLASATSLNPLPIHLISFSALKHN
                     NQVKLQWTAENELNFSHYEIERSGNGNSFTMITTVLAKGGMEQNGYEANDVQPLTGIN
                     YYRLKMVDKDGKFNYSIIIKVDLTKKYTVSIVPNPAHDYIIINGADNFRNIQLIDATG
                     KLVRQLNKTTANRYDIPGLNRGLYFVRLTALGETITVKVIIE"
BASE COUNT         3042 a         2390 c         2295 g         3207 t
ORIGIN      
        1 gggtaaacca atcagttttt agaacaattg ttttggctac ttataacaat acatgttgta
       61 taacaagtat caacaacact aatttattaa tagcaagcca cattgtacca tggagtaaag
      121 atcaaaaaaa caggcttaat cccacaaatg gtctttgtct gaatgcattg catgataaag
      181 cctttgataa aggtcttatt acaatttcag caaaagacta tacaattcgg gtctcttcaa
      241 aactcaaagg ggaaaaaaat ctccaattta ttgagctaaa ttttatagag tatgaaggga
      301 aaaaaatcaa ccttccagat aaatttttac cctcaaaaga acaattgaaa gttcataacg
      361 aaagctttat atcataacta ctcatgaagg acccatttgg catttactat aaaggcaaca
      421 acttttatat cgacatcttg cccgtctatt gtgctagaat ttttcatcgt taaaactaag
      481 tcttgaacat tttgctctat taatgaatag tctgttgata cattgtcggc atatttaaaa
      541 ttataggtgc tatatgctaa cccatttata aatagctgtg cattttttgt gtctgtaaac
      601 ttataggtaa ttgtagaagg agtatttaac attggcaatg cgggaatcaa ttttaatcca
      661 aaaagtacaa ggggatttgc tgataaaagt gttcctacac cagataacgt acctgcaaat
      721 gttttatatt ttgttctgga ttcctgccca accccaatcc aatatgccca gtaagttgta
      781 ttgctaggaa ggttaatctt aatcactgtt ctacttgaat gttccaagtt tgtggttgaa
      841 tacactctag ttacagtatt tagcacttct gtaaaagttg tatcgtaaac aattttgact
      901 gcagtgttaa agtttgggtt accatttttt gagggatttc tatctacagt aagtttagca
      961 ctcttagaaa agagaccttc ttgatacacc aggaaataat aaactccttt agtaggtata
     1021 gtgattttat cgtttactat tgatatgatt ctttcctttt tatatatttc tcttccagta
     1081 gaatactcaa agagagaaac ggtgaataac ccctttttgt taagcagcga agcgttaata
     1141 gtgacctcat catccttatc aaatccaatt acattttttt caagatcatt acccaatgca
     1201 ccagataaag caacttcttt atccagcact cgaatttttt ctattttact ttcaacatcg
     1261 catccgagct taatgattcc ttttgtgctt tcgtcaacat taatggtaaa attgtatttc
     1321 cctgttgtgt ttttaagttg aaggtagtgt tccccatatg aaagagtcag ctttttggca
     1381 tcatcagctt caaccgatcc aatttcattt ccatcaacta ctagaattcc gcttttgcaa
     1441 gcaactatta ggattgttgc ttctgggttc gattgacaaa agcaattgaa tgaaattaat
     1501 aggtatagaa tagctgaaat tacttgtttc attaaaaaga gtttatacaa gaataataaa
     1561 agaaaaacta gttttttagt gatttattaa tttattatca ataaaaaacc tttatttctt
     1621 aatttgtcag ccgttatcat ctccccacta aataaaagac ctagaccatc tccccagttt
     1681 gttttaagct ttttttagct tttgggtcat gtagaataaa taaagacccc ggctcaaccc
     1741 tgcattatgt tcagtgcata gccccgattg acgactgaaa aaaatgcgaa gcataagaga
     1801 gctcaaatag gtgccccata aatatccgcc attatttcca cccctttatg tgattgtaca
     1861 gccaggttcc cgcctctaac ttgtgctatc aataaagtat agccatgctc aacaacttct
     1921 ttcgtttact tattgtcctt tgctgtgcag gcggcacggc ccatgcacaa ctgaccaaag
     1981 aaacagttca gcagcatatc cgggaactgc tgcaaacagt gaccgataat tatgtgtacc
     2041 cggccattgc caaaaaagcc aagcaatatg tgctgaccct gcaggaaaag ggagcgtatg
     2101 atacgataca aaaaggagaa cggctggccg ctgcactgac ccgtgacctg caggccatca
     2161 cccatgacct gcacctgcgg gtattctatt cgcccactgt attgccaaaa caggagaaca
     2221 gcaacggccc ttcgccggaa gaaatagaag ggttcaaaca aatgctgaag ggggccaact
     2281 acggcattaa agaaaaatcc gtcctggaag ggaatatcgg ttacctgaac attaccatgt
     2341 ttgccccact ggatttttgt gccgacagtc ttacggcagc cgtgcaggca gtgatcaata
     2401 ccgatgcgct gatcattgac ctgcgggaat gtggcggcag catggatccg gatgccatcc
     2461 cctttttcag cggttatttt tttgaccggc ctacgcatct gaatgatctc tacttccgca
     2521 ataccggtga taccacgcag tactggagct atggctgggt gccggggaag aagtatggca
     2581 ataaacctat ttatatactc accagcggcc gcactttttc cggtgcggaa gagatcgctt
     2641 acgattataa gaatctgaaa agagccatcc tggtgggaga acaaacaaaa ggcggcgcca
     2701 atcccggcgg agatatgcgg gtcaacgatc attttggtgt atttgtaccg aatggctgcg
     2761 ccatcaatcc cattaccaaa accaactggg aaggagtggg agtaacaccg gatacagtga
     2821 tcagcgccat gaaggccctg cacaaagcac agctgatggc ggtacagcat ttatggcagc
     2881 aggcgaatac gccggaaaag aaaaaatcat tacaggaggc gttgacggcg atacaaaaag
     2941 ccgcaccggt ctataagcag gtacagttta ctttgaaggg atttgaaaaa gcgaaagagg
     3001 tatttgtggc cggcagtttc aatggatgga ccccagagca aacccccatg ctgagaaaag
     3061 gaaaggaatg ggtggccgtg gcggaagcgc cggtgggtaa actggcttac aaattttttg
     3121 tggatggaga atggatattg gatcccgccc atacacaaac agaatcagac ggccggtaca
     3181 ctaattcggt gaagcaggtg caataacccg ttttattcct ttttaacgat ggctatttta
     3241 tctcacagcg gaccagagtg tatcaatttc agcacaagtg cgtagaataa gtgctataat
     3301 ctacgcattg cctgtttggc tgcctgtaca tttttataac tatctattca gctgtcaaaa
     3361 ggcaggcttt cagggtcatt tttaacaacg taagtcaaaa ttgttgtttt ttgacttata
     3421 ttgagatgaa atgagtcaaa ttaagcctgc tgccattgag cttctcccat tgaaacaaga
     3481 attcgaaacc aaggctgtac ttcgaaaaac agccctggct caccaggcat tggctgaact
     3541 caaaggagta gtgaccagta ttcctaatca acatatctta acggagaccc ttacactgcg
     3601 ggaagcgagg gaaagctcag ccgtggaaaa tatcatcagc acttttgctg aagtatatca
     3661 aagtagttta ttctctaacc tgtttgcttc agcagcggca aaagaagtac atctttatgc
     3721 aagggcattg aaaaagggct accaactggt aaaacagcat ggcctgctga ccgggaatat
     3781 tattttgcag atacaggaac aagttgaagg aaacagtgcc gggtttagaa aattgcccgg
     3841 aaccaaattg ctcaatgatg ccaccgggga actggtatat ctccctcccc aggaccatga
     3901 aacaattgtg tcgttaatga ataacctgga aaagttcatt aatgatgata caatgatgga
     3961 tgcagaccca ttggtgaaga tggctatcat tcatcaccaa tttgagagca tacatccttt
     4021 ttatgatggc aacggaagaa cgggtcgtat cattaatatc cttttcctga tacaaaagaa
     4081 tttactggac atcccggtat tgtacctgag ccgctatatt attaaacata aaaatgatta
     4141 ctacaggttg ctgcaagcag taaggcaaac cggaaaatgg gaggagtgga tattgtacat
     4201 gctggacgga atagagcaaa cagccaggga aagcattatc ctgatcggac atatcaaaaa
     4261 cctgatgctg gaatacaaac aaacactacg tacgaaactg cccaaacttt acagccagga
     4321 tctgctcaac aacctattta aatatccgta cacaaaaatt gagtatctgg aaaaggaact
     4381 ggatataagc cgcagcacag ccatccggta tttaaaagaa ttggagaaac tgcatttagt
     4441 gtctaagcaa aaaattggac gggataattt ttatatcaat cagccgttgt ttaacctgct
     4501 tgcagctaat aaataaatat tatttttcat cttcggtata actgtccggg ttcaccgccg
     4561 gtgattgtgt caccacacca aattcaaatt gcatttcatt cagttttcgg taaagaccgt
     4621 tatgcgccat gagttcggta tgggtaccgg cttcgctgac gatgcctttg tcgatcagta
     4681 caattttatt ggcattgcgg atagtggaaa gccggtgggc tatcacaaaa gaagtgcggc
     4741 ccttcatcag gttgtctaat gcttcctgca ccagttgttc actttcacta tcgagtgagg
     4801 aagtggcttc atctaaaatc agaatagcag gatcttttaa gatcgccctt gcgatagcga
     4861 tgcgctgccg ctggccacca cttagtttaa taccccgctc tcctacaatg gtatcatact
     4921 gttccgggaa tcgctggatg aactcatgtg cattggcact tcgggcagct gctattattt
     4981 cttcttctgt ggcatgcggt ttaccatatg caatattttc gcggatgctg ccgccaaaca
     5041 agatcacttc ctgaggtaca aaggccattt gccggcgcaa ctgggaaaga ggaaattctt
     5101 ttgctggtcg ttcatcaaag tacacagtgc cactatctgg ttcataaaaa tgtaataata
     5161 aagaagcgat ggtggatttg ccggcgccac tcggtcccac gatggcgatc tgttccccgt
     5221 cttttgccgt cagtgtcagg tttcttaata ccggcacatc tttgcggctg gggtaactga
     5281 acgctacctg ttcaaacctt acattgcctt ttaatacata ttgtggctcc accacaatat
     5341 catctaactc caccgcctcg cctttgctgc gtaaaatttc ccggatgctt tgtgtggccc
     5401 ccagtgtttt ttgtaattgc gaaaacatat cagcaaagcc ggcgaaagta ccgccgacga
     5461 acatggagaa aataacaaac atagtgaggg cacccatcgt gagttcaccg gactgtatca
     5521 taccggcgcc tacccacatt acaaatgcaa tagtgccaaa cacactgaag atcataaacg
     5581 aaataaatgc accgcggtag cgggcattgc tgatggccgt gtgcaccacc gactgtatac
     5641 tttttacata gcggcctatc tcatgtatct cgctggtaaa ggcttttaca atggagatac
     5701 catgaaaggt ttcctgtaca atggtgccgc tgtcggccaa ttggtcctgt gcccggcggg
     5761 ccatcttgcg gatgcgtacg ccaaatacaa cggctagtat agcaatgacg ggcaccacgg
     5821 ccagcatcac cagggccagc ttggcactga tccagaaaat aaagaacaac ccgatgataa
     5881 gggtgaagat gcctcttaag aattcagcca gtgtaaaaga tatggcatcc tgtatctgcg
     5941 agagatcgga agagatgcgg ttgctcagct cccctactct tttttctgta aaaaaactca
     6001 tgggcatggt gagcagtttg ctgtacacat ctttgcgcat atcggccagt gaccgttcgc
     6061 cggctgacgt cagcaaatag acccgcataa aagagaaaat agtttgcagg gtcagttgac
     6121 aaaagataag gagcagggtg gtgtttaaac tccagtgaat attttttaag ccgaaatcaa
     6181 attgcgaagt agtcataccc ggtacctcac tacccggtga cgctgcatcg atcatcttgc
     6241 ccaggaacaa cgggaacaaa ctggtggtaa aagcagagag agcaatgaag atcagtcccc
     6301 cgatgaattt gccgcggtgc ggcttcaggt atttgaaaag gatgagtgcc tggcgcagac
     6361 tttcctttga gatctttgct ttggcagatc ctttctcttt tgtttcgctc aggtgtcggt
     6421 ttgcggtggc cataatttgt agtagtaagt cggtagcagg tatgcgggtt cgttgtatga
     6481 accttcttta ctgcaaggta attaatatgc acgggaggaa gagtgcctgt ggaatgaacc
     6541 ggtaatcgaa gttgttgaag ttatcccaac attgtttttg atgatcacag cagcgtacaa
     6601 cccggctgtt catttatctt atcagcacct catctataaa caagtgactc ggatagccgg
     6661 cactttcatg ccaggccggt aaagaaccgc cgctgatagc ttttactttt atataccttg
     6721 cttttgccat tacagctgca ccgatttgct ggtgaacagg accttttata tcagtaccga
     6781 tattattttt tacaatggtt aatggctgcc agttttttcc atcattactc ctttcaaaaa
     6841 tcacttcttt gggatatacg atccagggac tgacatcctg cagcacatgc acccctacat
     6901 actgcaagga tttttccttt tgcagatcaa tgatggcttc aaaatctttg gcataataac
     6961 tctgccagtc gccggtcttc cagttctcag tgcccgtaat gccatcgatc aaggcttctt
     7021 tgccaccggc ggtgtacatc ggatgtactt cacttaaaac agtgatgctg atctcagccg
     7081 gtattttata aaacttccgg taagtaaccg ggctttttat gccgttcttt tctgcatata
     7141 tcgttacccg gattgttttg tcaatcgtaa agggttttgt ataccggata aattctccgc
     7201 tactgccatc ttcctgttct gcactataat agctatcagc agcagcatcg atatgtttta
     7261 actgtacagc tgcagttgtt ttgaatttgt aactctccat atcaaaaaaa ggaaccgcca
     7321 caaactgatc atcattcacc acactgtgcg gcatatcttt ttcagcgatt ccccttgttt
     7381 tgccgggctt gttgttcatc gagaaaagaa gttcgccacc gttggctata tcactatgct
     7441 gtataaatgt ggcagcatgt tttttatcgt tgagcaatac atcttctaca taaaaatttt
     7501 ctttcttccg gttctttgct ttaatgataa acgatttacc attttccagg tgcaggatta
     7561 cttcatcaaa caaagttgta cccagcgcat attctccgct gccgggagta accgggtaaa
     7621 aacccatagc actcagtaca aaccaggcac tcatctggcc gcagtcttca ttaccaatca
     7681 gtccatcggg attattcgga taaaactcag tgcagatttg atgcaccagc tcctgtgtac
     7741 gccagggctt gccggcataa ttgaataaat aagccatgtg gtggctgggt tcattgccgt
     7801 gtgcatactg accgatcagg ccggttacat ccgcctgctc ccttccgctc agtttagtat
     7861 tggtgataaa aagttcttct aatttttttg caaacgcttc attgcctccg tgcagttttt
     7921 tcaggccgtc aatatcctgc tgtgcggtaa aggagtaatg ccaggcattg ccttctgtaa
     7981 agaaattatt tacttctgtt gcatcgaagg gctgatacca gaatccctgc actttaccac
     8041 ggatgtgcct ggtgcgggga tcaaagagat tgcggtattg taaagaacgg ttcaggtatt
     8101 ttttatagac ttcatcatta ccggtccatt gggcgaactg tgcaatgcac cagtcatcat
     8161 aggcatattc caccgtttta gaggcacttt catgatcatc ttcattgctg ataaaaccct
     8221 gtttcatata agcaggcaaa ccatagcggt tgctttctgc ataatccgtc atcgctttta
     8281 aagcaagctt tgtatcaaaa tcacggatac catttttata tgcatccaca attaccggca
     8341 cggcatgata accgatcata caaaaagttt cattgccact cagttcccac accggcatca
     8401 tgccgccatg ttgatactgg accaaaaagg tgttaatcca gtccagggtt cgttgccggt
     8461 taatgatgga atgcaaagga tgcaatgccc ggtgtgtatc ccagagagag aaaacagtgt
     8521 aattggtaaa accttcagca ttgtgcacct tcaggtcggt gccacggtat tcaccgttta
     8581 catcggtgta aatatttggt gccagcatgg tatgatacaa ggctgtataa aaggccacct
     8641 gctgatcagg cgtgccgccg ctgatggcta ttttgctgag ttctttgttc catgctgctt
     8701 cagcaacatc ttttacttta ttgaaattcc agtccggcag ttctgtatcc agattgagtt
     8761 tggcattctc cgtactaacg ccggagatac caacttttac ctgtacggtt ttattcccgg
     8821 caatagcgaa agcgaaatag gctttgatat ttttcccatt aattgatgct gttttaatgt
     8881 ctgttggttc taccggttca tccttgctgg caatgccata attttttacc ggctgatcaa
     8941 acttcaggta aaaataaacg acctggtttt gcgcccatga tttgctgcgg cgcattcctt
     9001 ttatttcata aggactgatt atttccaacg aggattcaag cacttcatcg cggtgttgca
     9061 ggtcaagtaa tatctttcct tccgttgttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnna accggaaaga tttcatagca gtgatattat tcgttgctca agttaacaga
     9241 aaatgaaaga cgattaaagt tttcgtcata acagctttgt gccaaaaacc ggtattgacg
     9301 atggcgattg tatggcagtc atgcgggttc gttgtacaaa cccttacgct gcaatttaat
     9361 ttaaaggtac gggaggaaga atgcggttgg aatgaatcgg cgtttgaaga tggtggttaa
     9421 gacactcctg acatttgaaa cccgggatcg tcgccgcaaa tgatggcaag tgagaccccg
     9481 ggtcgtattt aacaaacagt cggatcagca taaatcacct tactgtctgt ttaaatgttg
     9541 taccatatct ttttattatt caataatcac tttgaccgta attgtttcac caagtgccgt
     9601 taaccgaaca aaatacaatc ccctattaag acctggtata tcgtatcggt tggcagttgt
     9661 tttattcagc tgtcttacca gtttgccggt ggcatctatc aactgaatgt tcctgaaatt
     9721 atctgcaccg tttataataa tataatcatg tgcaggattc ggtactatgc tcacggtgta
     9781 ttttttagtc aagtccactt taataataat gctgtagtta aacttaccat ccttatccac
     9841 catttttaac cggtaataat taatgccggt cagtggctgt acgtcattgg cctcatatcc
     9901 gttttgttcc atgcctcctt ttgctaacac cgttgttatc atggtaaagc tgtttccgtt
     9961 gccacttctt tcaatctcat agtgtgaaaa gttcagttca ttttcggctg tccattgcag
    10021 ttttacctgg ttgttatgct ttaaggcgct gaaactgatt aaatgaatgg gcagtggatt
    10081 taaggaggtt gctgatgcta atgtaaacgg gctaaaatct gtaaccacac ctgcagtcag
    10141 aatggtaccg gctgtattgt taccggtggt tccgccattg ccttcgtctg tccacaaact
    10201 gccattccag cgggccactc tcaggtcgcc caggttattg attccaccgc tgcggcttgc
    10261 ttcccagctt aaagtaacag ctgttgcgga aacaccttct gttctgttca gcagccagta
    10321 ttcccttaaa gatatatgat ctatgccggg ccctttttgg gtggaatcgt aaccgctgtc
    10381 gtgcggtaca ctcataaagt attctgccct gaacgcatcg gtggtaaatg cgggtgctgc
    10441 tatgcttatg ggagcaattt tattttcttt tcccacagga aatataaatg catcgttacc
    10501 aattttttta caaggtccgt taacaaaact tatgttggat gccccgctat aattggcatt
    10561 attcaaaaat atcagttctt tacctgcact gctgttaatg ataccattga taaaactggt
    10621 gcttgtgcta atacttaccg tgtcattcaa tgtaattgtt ccgcctccgg ttttatctat
    10681 actcaattca gcaataatta caggctgtgt tgactgctgt tctataatac cgctggtgct
    10741 gccattcaac tttattttgc ccaaaccaat accgtcggtg gaatttaaag taaggttccc
    10801 gcctatctcg ttcacatcgc ctgcaccaac gctaatacct gcaccggttt tgatatacgt
    10861 tacattgccc aggtatttat ttccggtgcc tgcctggttg gcatccaata atacattgct
    10921 gccattgttg gcaatgctgg tattaccatt gatgatatta ctgcggaggt aagtataata
    10981 cccgcctgaa taactggcat cgttggcaat ggttacattt ccggtaatag catt
//
LOCUS       DAORTI010000116        11021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_6162_length_11021_cov_6.154660, whole genome shotgun sequence.
ACCESSION   DAORTI010000116 DAORTI010000000
VERSION     DAORTI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_11021_cov_6.154660"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLO70_12935"
     CDS             <1..636
                     /locus_tag="PLO70_12935"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HQZ75418.1"
                     /translation="FNQNQLTMNLIERAKNILLTPNKEWDVVNGEEPNIPGIITGYVL
                     PLAGAAAVAAFIGYGLIGFNYGFGIRIKGIDWGLYHAVTVLAGAILSVFVSAFIIDAL
                     APSFGSEKNLGRSVQLVAYSYTPGWIGGLLAILPSLAVIGSLAGLYGLYLLYIGLPKL
                     KKTPPDKHVSYFVVSLLVMIVVFVVIGLILGKLLMSVFGLSYGYGDFKISL"
     gene            complement(689..3553)
                     /gene="uvrA"
                     /locus_tag="PLO70_12940"
     CDS             complement(689..3553)
                     /gene="uvrA"
                     /locus_tag="PLO70_12940"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQZ75419.1"
                     /translation="MAKNNHSGTGISIQDNESIEVFGARVHNLKNIDISIPKNKLVVI
                     TGISGSGKSSLAFDTIYSEGQRRYMETFGAYARQFIGDMERPDVDKITGLSPVISIEQ
                     KTTNKNPRSTVGTVTEIYDFLRLLFARIGEAYSYNTGKKMVKWSEEEIVENIFQKFKG
                     KKIALLAPLVRGRKGHYRELFEDVRKKGFLKVRVDGEVKDITAKMQVDRYVIHDIELV
                     VDRLQVTDELKARLSQSVQQTLKLGKDLMFLLVDDSKFVQYSKLLMCEDSGISYEEPS
                     PNAFSFNSPYGACPTCKGLGAVYTIDMAEVLPDTSKSVKEGGIAPLGEERSAGVFNQV
                     VDFAKKNKIKLDIPVKDLGKAQLDLLLYGDKDINSSLEVDADDDTVPETYTGSYEGII
                     PMLKRWFTSSHSTEVLREWVQRFMTMKPCGTCNGARLKKESLWFKVNGRNIAELSNMN
                     LDNLAAWFDGIELMLDNKRKTIAKDVLKEIRERLQFLLDVGLTYLTLYRPSKTLSGGE
                     SQRIRLATQIGSQLQGITYILDEPSIGLHQRDNHRLIEALKNLRDIGNSVLVVEHDKD
                     IMLAADYLIDIGPNAGYHGGRIVAQGEPGELLKLDTLTSGYLNGHRRIAVPAERRNGS
                     GKFLELKGARGNNLQNVEAKFPLGKFICVTGVSGSGKSTLINETLYPILSKHAYGSKM
                     TPLEYKSVKGLEHIDKVIEIDQSPIGRTPRSNPATYCGFFTEIRTLFASIPEAKIRGY
                     NAGRFSFNVKSGRCEVCEGGGMRVIEMNFLPDVYVHCEKCNGKRYNRETLEIRYKGKS
                     IADVLEMTVEEAVAFFQNVPYLYRKIKVLEEVGLGYITLGQSAVTLSGGEAQRVKLST
                     ELSKRDTGKTFYILDEPTTGLHFEDIKHLLDVLNKLVDRGNTVLVIEHNMDVIKVADH
                     IIDIGPEGGDGGGRILFEGTPEELIKNKESYTAEFLKLEL"
     gene            3725..4300
                     /locus_tag="PLO70_12945"
     CDS             3725..4300
                     /locus_tag="PLO70_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75420.1"
                     /translation="MKKTIVLMGGIALSLLTVSVNAQETWDAKKNPTVDSISALYRDK
                     IVTAPPAQTREEIFPAIGKFESATNADAALITIAPDEQNKGVVWIEGLPQGKVKAMLR
                     KSPATYKIPAQKTEEGKDVAEGTLIFDKETNTLSICIGKIYNTTDPSAAFAATIEEPA
                     TTAKNSKVKKPVQPKAWIYTGTKLLKETARN"
     gene            4389..4928
                     /locus_tag="PLO70_12950"
     CDS             4389..4928
                     /locus_tag="PLO70_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75421.1"
                     /translation="MFNFFKKKGPVINVVDKVVINETAKLHTLLNLWKNDKNIAFIFW
                     FDESLRQAENYFSSQTTEPIPLLTAREAASPQLGGRTPVFAEHYPLRSREEELYLKMN
                     LENVQVFSSLKEPLFQQFGADKIIQVMQQLGMKEDEVIEHSMISNSIRRAQEKIEKKD
                     IIEQSAHSQKDWLEKNYKS"
     regulatory      5047..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5310..6635
                     /locus_tag="PLO70_12955"
     CDS             5310..6635
                     /locus_tag="PLO70_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQZ75422.1"
                     /translation="MSNNLHFETLQLHAGQQIDPTTKSRAVPIYQTTSFGFNNAAHAA
                     SLFGLKEFGNIYTRIMNPTTDVFEQRIAALEGGVAALATGSGQAAQFLALNNILQAGD
                     NFITTSYLYGGTYNQFKVAFKRLGIEARFADGDDAESFVKHIDKNTKAFYLETIGNPR
                     LNIPDFKKFADLAKEYDLPLIVDNTFGAGGYLFKPLEHGANVVVESATKWIGGHGTTI
                     GGVIVDGGNYNWGNGKFPQFTEPSEGYHGLKFWEVFGEGNPLGLPNIAFAIRARVEGL
                     RDFGASQSPFNSFLLIQGLETLSLRVQRHVDNALALAQWLEQHPAVEFVQYPGLKSSP
                     YHEQAKKYLSNGFGGVLNFGVKGTKENASKFIDSLKLVSHLANVGDAKTLAIHPASTT
                     HEQLNAEEQLTSGVLPNQVRISVGIEHIEDIKADFEQAFTTVFANELVG"
     gene            6637..7695
                     /gene="metX"
                     /locus_tag="PLO70_12960"
     CDS             6637..7695
                     /gene="metX"
                     /locus_tag="PLO70_12960"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQZ75423.1"
                     /translation="MTTIFNYTNTFTLESGETLPGFHLAYTTHGKLNASKDNVVWIFH
                     ALTANSNPVEWWPGLVGDGKFFDPAKYFIICVNKPGSPYGSISPLSKNPVSNQPYYDK
                     FPVFTIRDMVRSYQQLKEYLGIKKVFAGLGGSTGGMQLLEWAIEEPELFEHLIPIATN
                     AVLSPWGIAFNASQRMAIEADSTWQDHHQDAGHKGLAAARSIALLSYRHYNGYAITQR
                     RDKAFVPLNGDVNYAADNYQRYQGLKLVNRFNALCYYRLSQSMDSHDVGRNRNGAEAA
                     LKTIKAKTLVIGITSDVLYPISEQEFLQKTIPEAQLLGIYSDYGHDGFLLEYEKIESA
                     LQLFIDGKKSSHLKIVNQ"
     gene            7697..8953
                     /locus_tag="PLO70_12965"
     CDS             7697..8953
                     /locus_tag="PLO70_12965"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQZ75424.1"
                     /translation="METHKQLTIGLFGFGVVGEGLYKVLQQTPSLNAAIKKICIKNYG
                     KKRNAPASLFTTDKDELLNDHDINVIVEVIDDADAAFHIVTTALKNGKNVVSASKKMI
                     AEHLPFLLELQQQTGRSFLYESAACASIPVIRNLEEYYDNDLLHSIKAVVNGSTNFIL
                     TKIFEEGLDFKQALVLAQQLGFAESNPKLDVEGYDAVNKWTLLLAHAYGIIESPEKIL
                     FNGIQNIHTSDAVVAKAKNQQIKLVAQAQKIKNGKVSAFVLPQFVKQNDHLAFVKNEY
                     NGVVIESGFADKQFFYGKGAGSFPTASAVLSDIAALRYQYKYEYKKLYHHQPHELSNA
                     VFLRVYVSFDDIAFVPRERFEWIEEWHAQEERKYLIGVVSFDELKENNWWKENNTSLI
                     LTPDPVIEDIEIRKLKKKSLELAGIL"
     gene            9043..10011
                     /locus_tag="PLO70_12970"
     CDS             9043..10011
                     /locus_tag="PLO70_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQZ75425.1"
                     /translation="MKELSVVITVMNEEENIKPLLEAVRAALSGIDYEVILVDDGSAD
                     KTKQQVLEYADERTILVELRKNYGQSTAMTAGIDYSRGKYVALLDGDLQNDPTDIPAM
                     LELLKKEDWDVVAGNRKNRKDGFVLRKIPSKIANALIRHMTGVYIKDYGCTLKVFKRE
                     IAEDLGLYGELHRFIPVLAKLQGARITQVDVKHHARIHGKSKYGINRTFKVMSDLVLM
                     VFMRKYMQKPMHLFGTMGFISFGLGVLINIYLLVLKIMGQDIWGKPLLILGLILLLGG
                     IQFITIGIIADINVRTYFESQNKKTYNVRKIYHAKREISHTSDIAL"
     gene            complement(10012..10983)
                     /locus_tag="PLO70_12975"
     CDS             complement(10012..10983)
                     /locus_tag="PLO70_12975"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQZ75426.1"
                     /translation="MHIIVTGGAGFIGSNLIGTLFREYPGITITCIDNFDPFYSTKIK
                     QFNIRDFKNNPGFRLIQKDLAVTTGEELCELIKEPASAIVHIAAKAGVRPSIENPMAY
                     QQVNIIGLQHMLDFAKAKKIKQFVFASSSSVYGINDHFPWKEDEKLMPISPYASTKLS
                     GEMMGHVYSKLFGIRFIALRFFTVYGPGQRPDLAIHKFTKAILKGDPIPVYGDGSTSR
                     DYTFVDDTVRGIIGAINYTATDFEIINLGNNYTVSLKELVAAIEEVMGKKATIERHPE
                     QPGDVPKTFADISKAKALLGYNPQTPLQEGLKKFYNWFSENRALLIK"
BASE COUNT         3323 a         2331 c         2159 g         3208 t
ORIGIN      
        1 tttaaccaaa accaactaac aatgaactta attgaaagag caaagaacat cttgctgact
       61 cccaacaagg aatgggatgt tgtaaatgga gaggaaccta atattccagg tataataaca
      121 ggctatgtgc ttcccctcgc aggggctgct gctgttgctg cattcattgg ctatggctta
      181 atcggtttca attatggatt tggaatccga attaaaggaa ttgactgggg actttaccat
      241 gctgtaactg tactcgcagg tgctatatta agtgttttcg ttagcgcctt tataattgat
      301 gcacttgcac caagttttgg ctcagaaaaa aaccttggcc gttctgtgca acttgtcgct
      361 tattcttata cacccggttg gattggtggt ttactggcaa ttcttccgtc tttagctgtt
      421 attggtagcc ttgcaggtct atatgggtta taccttttat atattggatt acctaaatta
      481 aagaagactc cgccggataa gcatgtaagc tatttcgtgg tttctttgct tgttatgatt
      541 gtggtatttg ttgttattgg attaattcta ggtaaattac ttatgtctgt atttggtttg
      601 tcatacggat acggagattt caagatcagt ttataaaatc aataataaaa aaagcggcca
      661 gtttaaatct ggccgctttt ttatttagtt acaattccag cttcaaaaac tcagctgtat
      721 agctttcttt attctttatc agttcctcgg gtgttccctc aaataatatt ctccctcccc
      781 catcaccacc ttcggggcca atatcaataa tatgatcggc cactttaatg acatccatat
      841 tatgttctat gaccaggacc gtattgcccc tgtctaccag cttatttagt acgtctaaca
      901 ggtgttttat atcttcaaaa tgcaatcctg ttgttggttc atccagtata taaaatgtct
      961 ttccggtatc tcttttggaa agctctgttg ataattttac ccgttgtgct tctcccccgc
     1021 ttaatgttac agccgattgt cctaaagtga tatatcccaa acccacttct tccaagactt
     1081 ttattttccg gtacaggtaa ggaacattct gaaaaaacgc aactgcttct tccactgtca
     1141 tctccagtac atctgcaatg gattttcctt tatagcgtat ctccagtgtt tccctgttgt
     1201 atcttttgcc attacatttt tcacaatgca catatacgtc aggcaggaaa ttcatttcaa
     1261 tcacccgcat gcctccgcct tcacacacct cacagcggcc actttttaca ttgaaagaaa
     1321 accggcctgc attatatcca cgaattttcg cttcgggtat agaggcaaat aaagttctta
     1381 tctccgtgaa gaatccgcag taagtagcgg gattgcttct gggtgtccgg ccaatcggtg
     1441 actgatcgat ctctatcact ttgtcgatat gctccaatcc tttcaccgat ttatattcca
     1501 gtggagtcat ctttgaaccg tatgcatgtt tggagagaat agggtacagg gtttcattaa
     1561 tcaaagttga ttttccacta ccactcacgc cggttacaca gataaatttt cctaacggaa
     1621 actttgcctc tacattttga agattatttc cccttgctcc ttttagctca agaaattttc
     1681 cactaccatt ccttctttcc gccggcacgg caatccttct atgcccattc aaatagccgg
     1741 aagttagtgt atctaatttc aataactccc ctggttcccc ctgtgctaca attcttcctc
     1801 catgataacc ggcatttgga ccaatatcaa tcaaataatc agccgccagc ataatatcct
     1861 tatcatgttc taccaccaat acactattcc cgatatcacg tagatttttt aatgcttcta
     1921 tcaatcgatg attatctcgc tgatgcagcc cgatagaagg ttcgtccaat atataagtaa
     1981 taccctgcag ttgggaacca atttgtgtag ccaatcttat ccgttgcgat tcaccaccac
     2041 tcaatgtttt ggatggccgg tataaagtga gataggttaa accaacatcc aataaaaact
     2101 gcagccgctc ccttatttct tttaaaacat ctttggcaat ggtctttcgc ttattatcca
     2161 gcatcaactc aatgccatca aaccatgcag ccaggttatc caggttcata ttactgagtt
     2221 cagcaatatt tctaccattt actttaaacc aaaggctttc tttttttaac cttgcaccgt
     2281 tacaggttcc gcatggcttc atcgtcatga accgttgcac ccattcacgc aatacttccg
     2341 tactatggga agaagtgaac caccttttca acatagggat gataccttca tagctgccgg
     2401 tgtaagtttc cggcacagta tcatcatccg catcaacttc aagagaggaa ttgatatcct
     2461 tatcgccgta caataaaaga tccagttgtg ccttacccag gtctttaacc ggaatgtcta
     2521 acttaatctt attctttttt gcaaaatcca ccacctggtt aaatacgccg gcacttcttt
     2581 cttcacccag gggagcaata cctccttcct taacactttt agaagtgtca ggcagtactt
     2641 cagccatatc aatggtatat acagcaccga gccccttaca ggtgggacag gcaccatacg
     2701 gagaattaaa tgaaaaagca ttgggagatg gctcttcata gctgatgccg gaatcttcgc
     2761 acatcagcaa tttggaatac tgcacaaatt ttgaatcgtc taccagtaaa aacatcaggt
     2821 ctttacccaa tttcaaagtt tgttgcacac tctggcttaa cctggctttt aattcatccg
     2881 ttacctgcaa cctgtccacc accagttcga tatcatgaat aacatacctg tccacctgca
     2941 ttttggcagt aatgtctttt acttctccgt ccactctcac tttcagaaat cctttctttc
     3001 gtacatcttc aaataattcc cggtagtgac cctttcttcc tcttaccaag ggcgccagga
     3061 gggctatttt tttcccttta aacttttgaa aaatattttc tactatctct tcctcactcc
     3121 atttcaccat cttctttccc gtattataag aataagcttc gccgatcctg gcgaataata
     3181 aacggagaaa atcatatatc tctgtaacag tgccgacggt agagcgggga ttcttattgg
     3241 tggtcttttg ctctatagaa ataaccggtg acagcccggt gatcttatct acatccggcc
     3301 gttccatatc gccaataaac tgccgggcat aagctccaaa agtttccatg tacctccgct
     3361 gcccttccga ataaatggta tcaaatgcca gcgaagactt tccactgccg ctgatacctg
     3421 taattacaac caatttattc ttgggaatgg aaatatctat attcttaaga ttatgtaccc
     3481 ttgctccaaa aacttcaatt gactcgttat cctggattga tatgcctgtt ccgctgtgat
     3541 tattctttgc catacgatgg tgaacgaaga taagaacaac ctaagaatct aattgttgtt
     3601 gacattcccc ttcaaaattc aattgagtga ttaaagtttt acaagttatc cacaaaaaag
     3661 tagtctggca cagtattttg aagtattgcc ccgagtacca attcaataat caaaaaattt
     3721 tcttatgaaa aaaaccattg tgttaatggg cggaatcgct ttgtccttac ttacagtttc
     3781 tgtaaatgcg caagaaacct gggatgccaa aaaaaatccc actgttgatt ccatctcagc
     3841 tttatatcgt gacaagatag taactgctcc tcctgcacaa acaagagaag aaatcttccc
     3901 tgctattggc aaattcgaat ccgccaccaa tgcagatgct gcacttatta caattgctcc
     3961 tgacgaacag aataaaggcg tggtttggat tgaagggtta ccacagggga aagtaaaagc
     4021 catgttgcgt aaatcacctg ctacctataa aattcccgct caaaaaacag aagaaggtaa
     4081 agatgtagct gaaggcacat tgatctttga taaagagact aataccctga gcatatgtat
     4141 cggcaaaatt tataatacta ctgatccctc agcagctttt gccgcaacca ttgaagaacc
     4201 cgccaccact gcaaagaatt ctaaagtgaa aaagccagta cagcctaaag catggatata
     4261 taccggcacc aagcttttga aggaaacagc ccggaattaa aagcattgat tggacggttc
     4321 ataacagtaa tgaaagaggt tgtcaaatga caacctcttt cttcttaatt tagccggtaa
     4381 aaaccagtat gttcaacttt tttaaaaaga aaggaccggt aatcaatgta gttgacaaag
     4441 tggtaatcaa tgaaacagcc aagcttcata cactgctgaa cctttggaaa aatgataaaa
     4501 acattgcttt tatcttctgg ttcgacgaaa gccttcgcca ggccgaaaat tatttcagca
     4561 gtcaaaccac agagcccatc ccattactta ctgcaagaga agcggcatct cctcaactgg
     4621 gtggaaggac acctgttttc gctgaacact accccttgcg aagcagggaa gaagaactat
     4681 acctgaaaat gaacctggaa aatgtacagg tattttcatc actgaaggaa ccgttatttc
     4741 agcaattcgg ggccgataaa ataatccagg taatgcaaca actgggcatg aaagaagatg
     4801 aggtaattga acacagtatg atcagtaatt ccataagaag agcccaggaa aaaattgaga
     4861 aaaaagatat cattgaacaa tctgcccatt cacaaaaaga ctggttagaa aaaaattata
     4921 aatcctgatc tcctttgctg tactgctttt cagcccatat ccaaacacct gttcgcagaa
     4981 attttttact gactgatttg gaaatccgga atttcatcct atatctttgt accagttctt
     5041 tacagatact tatcaagaaa ggcagagggt aatggcccga tgaagccttg acaacctccc
     5101 ccggttatac ggggaaaggt gccaattcca ccccgacgga acatgtcggg acagataagt
     5161 cagattaata gcttctaata ttctcaacaa catattcaaa agctcatctg atttatcggg
     5221 tgagcttttt tgttgctctt cccggtagcc ttttttgtaa gtgatgtaaa aatgtttaac
     5281 aattttcatc atgtttaaaa tcaaaaaaga tgagtaacaa tcttcatttc gaaactttac
     5341 aactgcacgc cggccagcaa attgatccaa caacaaagtc aagagccgtt cccatttacc
     5401 agacaacttc ttttggcttt aataatgcag cacatgcagc cagtcttttt ggtctgaaag
     5461 aattcgggaa tatctatacc cgcatcatga acccgacaac ggatgtattt gagcaacgca
     5521 ttgcagccct ggaaggcggt gttgctgcat tagcaacagg ttcaggacag gccgcgcagt
     5581 tcctggcatt gaataatatt ttgcaggctg gtgacaattt catcaccact tcctatttat
     5641 atggaggcac ctacaaccaa ttcaaagtgg ctttcaaacg cctgggcatt gaggcaagat
     5701 ttgcagatgg tgatgatgca gaaagttttg taaagcatat tgataaaaac acaaaagcat
     5761 tttatttaga aacaatcggt aatccacgtt taaatattcc tgattttaaa aaatttgctg
     5821 acctggcaaa agaatatgat ttgccgctga tagtagacaa tacattcggt gccggcggct
     5881 atttatttaa accattagag catggtgcca atgtagtagt ggaatcagca acgaaatgga
     5941 tcggtggtca tggtactact attggtggag ttatcgttga tggcggaaat tacaattggg
     6001 gtaatggcaa gtttccccag ttcactgaac cttcagaggg ttaccatgga ttaaaattct
     6061 gggaagtatt cggagaagga aatccactgg gactacccaa tatcgctttt gctatccgtg
     6121 ccagagtgga gggattaagg gatttcgggg cttcacaaag tccgttcaat tcttttctac
     6181 tgattcaagg gctggaaaca ttgagcctac gtgtacaacg tcatgttgat aatgcattgg
     6241 cattggcaca atggctggag caacatcctg ccgttgaatt tgtacagtat cccgggctca
     6301 agagcagccc ctatcatgaa caggctaaaa aatatctcag taatggcttt gggggtgttc
     6361 ttaatttcgg cgtaaaggga acaaaagaaa atgccagcaa atttatcgat tcattaaagc
     6421 tggttagtca cctggccaat gtaggtgatg cgaaaacact ggctattcat cccgcttcta
     6481 ccacacatga acaattgaat gctgaagaac agcttacttc aggtgtattg cccaaccagg
     6541 ttcgtatcag cgttggtatt gaacatatag aggatatcaa ggcagatttt gaacaggcat
     6601 tcaccaccgt atttgcaaat gaattagtgg gctgatatga caaccatttt caactatact
     6661 aatacattca cattagagtc aggtgaaacc cttccgggct ttcacctggc ctatactaca
     6721 catggcaaac taaatgcttc aaaagataat gtggtttgga tatttcatgc attgacagcc
     6781 aacagcaacc cggtagaatg gtggccggga ttggttggtg acggaaagtt ttttgacccg
     6841 gcgaagtatt tcatcatttg tgtgaataaa cccggaagtc cttatggcag tatatcgcct
     6901 ttgagcaaga atccggtctc gaaccagcct tattacgata aatttcctgt ttttacaatc
     6961 agggatatgg taagatcata ccagcaactg aaagaatatc ttggtattaa aaaagtattc
     7021 gctgggctcg gtggcagtac aggcggtatg caattgctgg aatgggcaat agaagaaccg
     7081 gaattgtttg aacacctgat tcctattgct accaacgctg tcttatcacc ctggggcatt
     7141 gccttcaatg cgtcacaacg gatggctatc gaagcagaca gtacatggca ggatcatcac
     7201 caggatgcag gccacaaggg attagcagca gcccgttcta ttgccctgct ctcctaccgg
     7261 cattataatg gttatgcaat tacacaaaga agggacaaag cgtttgtacc acttaatgga
     7321 gacgttaatt atgcagctga taattatcag cgttaccagg gattgaaact ggtaaaccgg
     7381 tttaatgccc tttgttatta ccggctttct caaagcatgg acagtcacga tgtaggccgg
     7441 aacaggaacg gtgcagaggc tgccctgaaa acaataaaag caaaaacact tgttataggc
     7501 attacatctg atgtattata tccgatttcc gaacaggaat ttttacaaaa gacaattcct
     7561 gaagcacaat tattaggtat ttacagcgac tatggccatg atggtttttt gctggaatat
     7621 gaaaaaatag aatctgcctt acaattattt attgacggga aaaagtcatc tcatttaaaa
     7681 atagtgaatc aataatatgg aaacacataa acaactgaca atcggcctgt ttggttttgg
     7741 tgtagtagga gaaggtcttt acaaagtttt acagcaaaca ccctccctta atgcagctat
     7801 aaaaaaaatc tgcatcaaaa actatggtaa aaaaaggaat gcaccggcct ccctcttcac
     7861 cacagacaaa gacgaattat taaacgatca tgacatcaac gttatcgtag aagtaattga
     7921 tgatgcagat gcagcattcc atatcgtaac gacagcattg aagaatggga agaatgtggt
     7981 aagtgccagt aaaaaaatga ttgctgaaca tttacccttt ttattggaat tacaacaaca
     8041 aacagggagg tcattcctgt acgaatctgc tgcctgtgca tctatcccgg tcatacggaa
     8101 cctagaagag tattatgaca atgacctgtt acattccatt aaagctgttg tgaacggctc
     8161 taccaatttt atactgacaa aaatatttga agaggggctt gatttcaaac aggctttggt
     8221 gctggcccaa caactgggat ttgccgaaag taatcccaaa ctggatgtgg aaggctatga
     8281 tgccgtcaac aaatggacat tactgcttgc acatgcctat ggcattattg aatcaccgga
     8341 aaaaatttta ttcaacggaa ttcaaaacat acatacttcc gatgccgttg ttgccaaagc
     8401 aaaaaaccaa cagatcaaac tggtagccca ggcacaaaaa ataaaaaatg gaaaggtctc
     8461 cgcatttgtt ttaccacaat ttgtaaaaca gaatgaccac ttagcctttg taaaaaatga
     8521 atataatgga gtggtaatag aaagcggatt tgcagacaaa caattctttt acggaaaagg
     8581 ggccggcagt ttccctaccg catccgctgt gctgagtgat atagcagcat tacggtacca
     8641 atataaatat gagtataaga aactatacca tcatcaacct catgaattga gtaatgctgt
     8701 tttcctgcgg gtttatgtaa gctttgacga tattgccttt gtaccccgtg aacgatttga
     8761 atggatagaa gaatggcatg cacaggaaga gagaaaatac cttattggtg ttgtctcttt
     8821 tgatgaattg aaggaaaata actggtggaa agaaaacaat acttccctga tccttacacc
     8881 agaccctgtt attgaggata ttgaaatcag aaaattaaag aagaagagcc tggagctggc
     8941 aggtatactc taattttaaa tttgctatct ggttaaagag ggtttgtgaa agcaaactct
     9001 ctttgtattt gaaaatgtac ctttgcccaa tatttaacca gcatgaaaga attatcagtt
     9061 gtcatcaccg taatgaatga agaggagaat attaagcccc tcctggaagc agtccgtgct
     9121 gcactcagcg gtatagatta tgaagtaata ctggtagatg atggttctgc tgataaaaca
     9181 aaacaacagg tactggaata tgctgatgaa agaacgatct tagtagagtt gcgaaaaaat
     9241 tatgggcaga gtacagccat gacggccggc attgattatt cccgcggaaa atatgtggca
     9301 ttgcttgacg gagacctgca gaatgacccc actgatattc cggcgatgct ggaactattg
     9361 aaaaaagagg actgggatgt cgttgccggt aaccgtaaaa acaggaaaga cggatttgtt
     9421 ctccgcaaaa tacccagtaa aatagccaat gcccttatac gccacatgac aggagtatat
     9481 atcaaggatt atggttgtac gcttaaagta ttcaaaagag agatcgctga agacctgggc
     9541 ttgtatggcg aactacatcg ctttattcct gtactcgcaa aattacaggg tgccagaata
     9601 acccaggttg atgtaaaaca tcatgcccgt atacatggta aaagcaaata tggtattaac
     9661 cgcaccttca aagtaatgag cgacctggta ctgatggtct tcatgcgaaa atatatgcag
     9721 aaacctatgc acctttttgg taccatggga tttatcagtt ttggattggg tgtcctcatc
     9781 aatatttacc tgctggtatt aaaaattatg ggacaggata tctggggaaa acccctgctg
     9841 atcctgggac tcattctttt attaggcggc atacagttta ttacaattgg tatcattgca
     9901 gatatcaatg tgaggaccta ttttgaatcg cagaataaaa agacctataa tgtcaggaag
     9961 atttatcatg ccaaaaggga gataagtcat acctcggata ttgccttgta attactttat
    10021 aagtaacgct ctgttttcag aaaaccagtt atagaatttt ttaagtccct cctgtagtgg
    10081 ggtttgagga ttataaccca acaaagcttt tgcttttgaa atatcagcaa aggttttagg
    10141 cacatcacct ggttgctcag gatgtctttc aatagttgct tttttaccca tcacttcctc
    10201 tattgcagca actaactctt taagggatac agtatagtta ttaccaaggt taataatctc
    10261 aaaatccgtt gccgtatagt tgatagcgcc aatgatccct cttacagtat catccacaaa
    10321 agtgtagtcc ctgctcgtac tgccatcgcc ataaacaggt ataggatctc ctttcaaaat
    10381 ggctttggtg aatttatgaa tggcaaggtc tggccgttga ccgggtccgt aaacagtaaa
    10441 aaatcgcagg gcgataaacc ggatgccaaa caatttgcta tatacatgtc ccatcatttc
    10501 accggaaagt tttgtactgg catagggact gataggcatc agtttttcat cttctttcca
    10561 gggaaagtga tcattgattc catacacact gctgctggag gcaaaaacaa attgtttgat
    10621 ttttttggct tttgcaaaat caagcatatg ctgaagacca ataatattca cctgctgata
    10681 ggccatcggg ttttctatac tgggtctcac cccggctttg gcagcgatat gtacaattgc
    10741 actggcaggt tctttaatca gctcacataa ttcttccccg gttgttactg ccaggtcttt
    10801 ttgtattaac cggaaaccag ggttattttt aaaatcccgg atattgaatt gcttaatctt
    10861 tgttgaataa aacggatcaa aattatcaat gcaggtgata gtgattccgg gatactcccg
    10921 aaacaatgta ccgatgaggt tgctgccaat aaaaccggct ccgcctgtaa cgataatgtg
    10981 cattgttatt ttctaatatg ccttaaagta aaaaatcctg c
//
LOCUS       DAORTI010000117        10889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_6273_length_10889_cov_9.827672, whole genome shotgun sequence.
ACCESSION   DAORTI010000117 DAORTI010000000
VERSION     DAORTI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6273_length_10889_cov_9.827672"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLO70_12980"
     CDS             complement(<1..1235)
                     /locus_tag="PLO70_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQZ75427.1"
                     /translation="MIKHNSSGLQVFILGILLFAGLISCQKKETLFTSLSSSKTNITF
                     TNNLEKHKLFNILYYLYYYNGGGVATGDINNDGLPDIYFTANNKSGNKLYLNKGNFEF
                     EDITDKAGVAGKTDWCTGVSMADVNGDGFLDIYVSSVNNKYELKGHNELFINNGNGTF
                     TEKSAAYGLDYSGLTTQTAFFDYDHDGDLDCYVLNQSHHPHSNIVDTNNRKRYDSLSG
                     DILYRNDVNNGGKFIDISREAGIYQSNLGYGLGIAIADINNDGWEDIYVGNDFHENDY
                     YYINNGNGTFTESGAKHFNHYSRFSMGNDVADYNNDGQLDVVTVDMLPPDEKILKTYG
                     SDENPDIYKVKLEIQGYQNQYSRNSLQRNNGNGSSFSEMALMSGVSATDWSWAPLFAD
                     FDNDGNKDLFISSGIVKRPV"
     gene            complement(1355..2203)
                     /locus_tag="PLO70_12985"
     CDS             complement(1355..2203)
                     /locus_tag="PLO70_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQZ75428.1"
                     /translation="MKKNIQQYLLAATVVLLAASCQKMDRPALGDFPKDTNPPGGPLK
                     FYAAFDGTTSDPLMNAVDSIRANFAGSNPLASVDGVSGKAVKGESQKFIRYAKPNDWA
                     KLAKSFTISFWYKRDGQTKNNTGTNGPEYPVSFKSNNGHWSGANMFVIIEGDNAACAV
                     KVMIADSSNADNWFTWEGGNTIPGLLNNNWRHMALVYSAATSGMTLYIDGVANSNVRV
                     WGTHGDIKIDDTKISEMRIGAGPGTNYSSDDWLSSSWKGELDQFRMYDVALTASEIQG
                     LFNGRK"
     gene            complement(2246..3754)
                     /locus_tag="PLO70_12990"
     CDS             complement(2246..3754)
                     /locus_tag="PLO70_12990"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQZ75429.1"
                     /translation="MRNFKSSFFALLLALPLLMLLSGCEKFLDRKPLTATLEDLNQGG
                     IEAEIFGIYSNLRNSPGFTTIPWLGVHDFRSDDSEKGSDAADGAEWVAPFDNYQYVKD
                     LWATNVYWDDHMTLIQLANAAIHKADSLGLTDASTILNIAEARFMRAFSYFDLVRTFG
                     EVPIHPGKIYQSSDLCKPKSTIAQVYAFIDADLQFAADNLPATWGLQYPGRLTTGAAK
                     ALHAKTHLYRQNWAQALALCQQVMGSATYSLNSSYYGIFKDAGENSGESIFEIQAYVS
                     PNGSIDQGCGFATTQGVRAATATGWNLGWGWNTPTDNLVNAFEAGDVRKGSTILFSGQ
                     SDDPSTGGYGRVLPNYILDPVPGPLPRKYWNKKIYADPAYRNSTGFSDNPRWINKRII
                     RYADVLLMAAEASNELGQGATGIGWLNSVRTRASLTGRSYTTQAQLRADIKHERRVEF
                     GVEGERFFDLVRWGDALTVLGPQGYTNRCRYYPIPQPVIDRCGGTIIQNPEW"
     gene            complement(3773..7144)
                     /locus_tag="PLO70_12995"
     CDS             complement(3773..7144)
                     /locus_tag="PLO70_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75430.1"
                     /translation="MKKNETTAVSKKDHLYQKIARIMKLTFAFLLIACLQVSARGFSQ
                     DRITLKMNEAEIKKVLFAIEKKTNYRFLFNESAFKRKPKVTIDAVDASVTDLLDKILA
                     NTGVSYKILGSKLVVLKEGIASGEIASQDLRVSGKVTGDNGEPLVGVSVSVKGSRTGT
                     TTDANGNFSLTVPDDAVLVFSSVGYESVEMAVAGKSTLNVSMLQSSRKIDEVVVIGYG
                     TASKRDLTGSIVKVAGKDIADKPNTNPVSSLQARVAGLTIINNGTPGAAPDIRIRGTN
                     SLGGNLQPLYVVDGIFNDNIDYINPSDIESIEVLKDPSSLAIFGVRGANGVIAVTTKK
                     GKAGQTVINFNTAFGFKKLVDKIAWANASEFQTLYEEEKVNIGANSPFDYGKWTANTD
                     WVDAVTRTGKFNTNNLSIATSTEKNRFYMGVGYTTDEGIIKHEKLKKLQLTITDELKV
                     NKFLKLGFNINTVKQDNPYGASWVLDAARKVIPQVSAGTKSVFTKNPYGLDSNNYNLY
                     YELPIIQNSGVINPLLILENEYNKTISQENRIVASAFAEFTLHRNLTFKSTVYADMSS
                     VNARVYTPLYNAYDAANDTTFLYSRRSGVRESDDTYKKFQTDNILTYKGRIASDHNIT
                     GMVGFTTYDFRYAGRFGSSQQSITGNAVPDDKRFWYVTNGFEDPLSVRASSAQYQRTT
                     ASFLTRALYNYKGKYYFNASFRMDGTSVFPNNRWQPFWAIGAAWELTKEEFMQEQNVI
                     DFLKLKASTGVLGVQNTGGYNYPAYPLLRTGSAAVFGDLVYTAAENDYLADENLKWET
                     NKANEIGVELDAFKRRLHFEAAYYSKKTEGLLSRVNNGGLSDGLLNQGALKNSGMEFS
                     ASWTQSLNRDLSITVGGNLTTFNNKVLSLAGDGKDIFDGPSITRVGSPIGSFYGYVVE
                     GIYQSYAEKLASPVNTEFSYGPGDLKYKDINGDGVINTDDKTIIGNPTPDFTYGGNVS
                     VKYKGFDLGLDFGGVYGNEIFRVWGATESPFQRVNYPKFKINRWTGEGTSNWDPILGQ
                     DHRINYENSTYSIEDGSYFRLRNVQVGYNVEPKFISKLKMKSLRLFFNMQNAKTWKNN
                     LGYSPEFGGSATSFSVDRAGNAIPMVTTFGLNVNF"
     gene            complement(7292..8404)
                     /locus_tag="PLO70_13000"
     CDS             complement(7292..8404)
                     /locus_tag="PLO70_13000"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQZ75431.1"
                     /translation="MSQELFWVLFSKKLSGEASAEEMGLLENLIQQHPEWQYAIQNLE
                     DLWKYQPYKDFVQEEDAYMLHLHRMAELNIPFGEVAIEAEIPAKERNRLVTKWYWAAA
                     AVIALVGLFAFGGLFNDEPERNPIAQQVNEITTRPGSKSKVQLPDGSVVWLNAGSKLT
                     YNKDFGKEIREVTLTGEGFFDVAKNKEKPFVINTTTISIKVLGTAFNVKAYPDDKQTE
                     ASLIRGSIEVTIRSRPNDKIILSPSEKLVVENNVPKADVREMRTGNIERTSLPVISTL
                     ISVNKLKYSPVDSTVAETQWVNNKLVFRNESFTELAVRMERWYDVAIDITDESIAEER
                     LNGIFETETISQALDALKETIPFQYEKTGNKIIIHR"
     gene            complement(8465..9058)
                     /locus_tag="PLO70_13005"
     CDS             complement(8465..9058)
                     /locus_tag="PLO70_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HQZ75432.1"
                     /translation="MLENEPIKRLLSAIAFTNDQAAYKELFLLLHGRLKQFAYSILKS
                     QEEAEELVSDLFIKVWEKRDQLTTIDSPLLYFYATAKNLAFNRLNKQKRQQNIRPEEW
                     LVQLNSIYFDPEQLMMTEEMMRQIRQAVNNLPPRCRLIFKLVKEDGLKYKEVAELLQL
                     SIKTIEAQMAIALRRIGKYMHMEVNQLSPDANNVKKS"
     gene            9152..9225
                     /locus_tag="PLO70_13010"
     tRNA            9152..9225
                     /locus_tag="PLO70_13010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9186..9188,aa:Ile,seq:cat)
     assembly_gap    9237..9336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9383..10612
                     /gene="hflX"
                     /locus_tag="PLO70_13015"
     CDS             9383..10612
                     /gene="hflX"
                     /locus_tag="PLO70_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQZ75433.1"
                     /translation="MLIPRSAGGVFIIMLDKRNVIQNEEKAVLVGVVLKDQTDTQVQE
                     YLDELAFLAETAGAVTVKRFTQKLQHPDSRTFVGKGKLEEIKKYVQGKDIRIVIFDDE
                     LSGAQINNIEKALDVKVIDRSDLILDIFARRAKTAQAKAQVELAQYQYILPRLRGMWK
                     HLERLGGGIGTRGPGETEIETDRRIVRDKISLLRKRLAEIDKQAYTQRKDRGEFIRVA
                     LVGYTNVGKSTIMNLLSKRDVFAENKLFATLDTTTSKVVFDTTPFLLSDTVGFIRKLP
                     HHLVESFKSTLDEVREADILLHVVDISHPNYEEQIGVVNKTLQELNAFEKPTLTIFNK
                     MDLYEKQTFDEWLEESTKKEILEDLYERWQRETNGHAVFVAAIEKRNLETLRKTILEK
                     VRELYRIRYPYKTEFLY"
     gene            10616..>10889
                     /locus_tag="PLO70_13020"
     CDS             10616..>10889
                     /locus_tag="PLO70_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQZ75434.1"
                     /translation="MTEKKYTWYKIAGHINELDFADNNIAVTELKGKKICIGRHQEAV
                     FAFAYKCPHAGGILADGHIDALGNIVCPLHRYKFNMQNGRNVSGEGY"
BASE COUNT         3067 a         2422 c         2075 g         3225 t
ORIGIN      
        1 accggacgct ttacaatacc gcttgaaata aacaaatctt tgttgccgtc attatcaaaa
       61 tccgcaaaca ggggcgccca gctccaatcc gttgccgaca ctccgctcat caatgccatt
      121 tcgctaaagc tgctgccatt accattattt cgctgcagcg aatttcttga atactgattc
      181 tggtatccct gtatttccag ttttacttta tagatatcag gattttcatc gcttccgtat
      241 gttttaagaa tcttctcatc gggtggcaac atatctactg tcactacatc cagttgcccg
      301 tcattattat aatcagccac gtcattgccc atgctaaagc gactgtaatg attgaaatgt
      361 ttggcaccgc tttccgtaaa tgttccgtta ccattattga tataataata atcattttca
      421 tgaaaatcat taccgacgta aatatcttcc catccgtcat tattaatatc tgctatggct
      481 attcccaagc cataaccaag attactctgg taaataccgg cttccctaga aatatcgata
      541 aacttaccgc cattgtttac atcattccgg taaaggatat caccggacag ggaatcatat
      601 cttttccggt tattggtatc gacgatattg gagtgaggat ggtgtgactg gttcaataca
      661 tagcaatcaa gatcgccgtc atgatcataa tcaaagaaag ccgtttgggt agtgagacca
      721 gaataatcaa gaccatacgc tgcactcttt tctgtaaaag tgccattgcc attattgata
      781 aaaagttcat tatgaccttt tagctcatac ttgttattaa ccgagcttac atagatatca
      841 aggaatccat caccgtttac atcagccata gaaacaccgg tgcaccagtc agttttacca
      901 gctacgccgg ctttatctgt aatatcctca aattcaaaat tccctttgtt aaggtatagt
      961 ttgttaccgc ttttattatt ggcagtaaaa tagatatcgg gcagaccatc attattgata
     1021 tcacctgttg ctactccgcc tccattatag taatagagat agtagagtat attaaataat
     1081 ttgtgctttt ccaggttatt ggtgaatgta atattggtct ttgaagatga aaggctggta
     1141 aacagtgttt cctttttttg acaactaatt aaacctgcaa acaacagtat ccccaatatg
     1201 aatacctgta gccctgaaga attatgcttg atcatatggc aaatttctat gtaataaacc
     1261 tgataaagtt caaaagggct gaaacaataa attgttcagc ccctttttta ctgcttatgc
     1321 tctgcgattg ccaaaaaaaa cttagcaaat catgttactt tcttccgtta aataatcctt
     1381 gtatttcgga tgccgttaaa gcaacatcat acatccggaa ctgatccagc tcacctttcc
     1441 atgatgaaga cagccagtca tctgaactat aattggtacc gggtccggca ccgattctca
     1501 tttcgctgat cttggtatca tcaattttaa tatcgccgtg agtaccccat actcttacat
     1561 tactgttggc aactccgtca atataaaggg tcatgccgct ggtggctgca ctatatacca
     1621 atgccatatg gcgccagttg ttattcaaca atccggggat tgtgttacca ccttcccagg
     1681 taaaccagtt gtcagcatta ctcgaatcag caatcatcac tttaacagca caggctgcat
     1741 tgtcaccttc tatgatcaca aacatattgg cacctgacca gtgaccatta tttgacttga
     1801 aactaaccgg gtattcagga ccgttcgtgc ctgtattgtt ttttgtttgt ccatcccttt
     1861 tataccaaaa tgaaatcgta aaactttttg caagttttgc ccaatcattc ggctttgcgt
     1921 acctgataaa cttttgactt tctcctttca ccgctttacc actcactcca tcaacagaag
     1981 ctaatgggtt gctgccggca aaatttgccc tgatactgtc tacagcattc atcagcggat
     2041 ctgacgttgt accatcaaaa gctgcataaa actttaatgg cccgccggga ggatttgtat
     2101 ctttcggaaa atcacccaga gccgggcgat ccattttctg acaactggca gccaataaaa
     2161 caactgttgc cgccagaaga tattgctgaa tgtttttttt catattcatt ttttaagatt
     2221 ttctgtttaa aaaatattta aacgattacc attcggggtt ttgtatgatc gtaccaccac
     2281 atctgtcaat aacaggttgt ggtatcgggt aatatcggca acggttagta taaccttgtg
     2341 gccccaatac ggtaagggca tcaccccatc ttaccaggtc aaagaaacgt tcgccttcta
     2401 ctccaaactc tacccttctc tcatgtttaa tatcagctct taactgagcc tgtgtggtat
     2461 aagaacgacc cgttaagctg gcccttgttc ttacactgtt aagccagcca attcctgttg
     2521 caccctggcc tagttcatta cttgcttctg cagccatcag taatacatca gcatagcgga
     2581 tgatcctttt attgatccat cttgggttat cactaaatcc tgtagaatta cggtaagccg
     2641 gatcggcata aattttcttg ttccagtatt ttcttggcag cggccccgga acagggtcta
     2701 aaatataatt aggcaacact ctgccatatc ctcctgtgga aggatcgtct gactgaccgg
     2761 agaaaagaat ggttgaacct tttctaacat cacctgcttc aaaagcattc accaggttat
     2821 ctgttggtgt attccatccc caaccaaggt tccatccggt agcagtggct gctcttactc
     2881 cctgcgtggt agcaaaacca caaccctggt caatagaacc attgggacta acataagcct
     2941 gaatttcaaa gatagactcc ccgctattct caccggcatc tttgaaaatg ccataataac
     3001 tggaattaag tgagtaagta gctgagccca tcacctgctg acataatgct aatgcctgtg
     3061 cccagttttg cctgtataaa tgtgttttgg catgtaatgc tttagctgca cctgtggtga
     3121 gccttccggg atattggagt ccccatgtcg caggcaggtt atccgccgcg aattgaaggt
     3181 cagcatctat aaatgcataa acctgtgcaa tggtactttt tggtttacaa agatctgatg
     3241 actggtatat tttaccagga tgaataggca cttcaccaaa ggttctcacc aggtcaaaat
     3301 aagagaaagc tctcatgaac ctggcttctg ctatgttcag gattgtgctt gcatcagtga
     3361 ggcccaatga atcagcctta tgaatcgctg cattggccag ctgaattaaa gtcatatgat
     3421 catcccagta cacattcgtt gcccaaagat cttttacata ctgataatta tcaaaaggag
     3481 ccacccactc agcaccatca gccgcatcac tacctttttc tgaatcatca gaacggaaat
     3541 catgcacacc gagccaggga atggttgtaa aaccaggact gttccggagg ttactgtaaa
     3601 taccaaatat ctcggcttca ataccaccct ggttcaaatc ctccagcgtc gctgtcaggg
     3661 gtttgcggtc caaaaacttc tcacagccac tcaatagcat caacagtggc aatgcaagca
     3721 acaatgcaaa aaaactactc ttaaaatttc tcattttttg ctatttaatt gtttaaaaat
     3781 ttacgtttaa tccgaatgtc gttaccattg gtatcgcatt acctgctctg tcaacagaga
     3841 atgaagtggc agaaccacca aactccggag aatagcccag gttatttttc catgtcttcg
     3901 cattctgcat attaaagaac aggcgaagac tcttcatttt aagtttggag atgaacttag
     3961 gttctacatt gtaaccaacc tgtacatttc tcaacctgaa gtaacttccg tcttcaatgg
     4021 aataagtaga gttctcgtaa ttgatcctgt gatcctgtcc taagatcgga tcccagttgg
     4081 aagtaccttc accagtccac ctgttgatct tgaacttcgg ataatttacc cgctggaaag
     4141 gagattcagt agcaccccaa acacggaata tctcattgcc atacacacca ccaaagtcga
     4201 gtccgagatc aaagcctttg tattttacag aaacattacc accataggtg aaatcaggag
     4261 tggggtttcc gataatggtc ttatcatctg tattgataac accatctcca tttatgtctt
     4321 tatacttcag gtcaccgggt ccataagaga attctgtatt taccggtgaa gccagtttct
     4381 ctgcataact ctggtaaatt ccttctacta catacccata aaaagaaccg attggagaac
     4441 ctactcttgt gatagagggg ccatcaaaga tgtcctttcc atcacctgcg agggagagta
     4501 ctttattatt gaaggtagtc aggttaccac ccactgttat actcaggtcc ctgttcaggc
     4561 tctgagtcca gctagcagag aattccatac cgctattttt caaagcgccc tggttcagca
     4621 aaccatcgct caaaccacca ttattaactc ttgataacaa gccttcggtt tttttagaat
     4681 aataagccgc ttcaaaatga aggcgacgct taaacgcatc caattcaaca cctatctcat
     4741 tagctttgtt cgtttcccat ttcagatttt catctgccag gtaatcgttt tcagcagcag
     4801 tataaaccag gtcgccaaac acagcagcag aacctgttcg taacagcggg tatgccgggt
     4861 agttataacc gccggtattc tgaacaccca atacaccagt agatgctttc agttttaaga
     4921 agtcaatcac attctgctcc tgcatgaatt cttctttggt cagctcccag gctgcaccaa
     4981 tagcccagaa tggctgccac ctgttattag ggaatactga tgtcccatcc atccggaagg
     5041 aggcattaaa gtagtacttg cccttatagt tatataaagc cctggtaagg aaagaagcgg
     5101 ttgttctttg atactgagca gaagaggctc ttacactcag cggatcttca aatccattag
     5161 ttacatacca gaatctttta tcatcaggaa cagcattacc ggtaatgctt tgctgagagg
     5221 aaccgaaacg accggcatag cggaagtcat aagtggtaaa acctaccatc ccggtgatat
     5281 tatggtcact ggcaatacga cctttatagg ttaaaatatt atctgtctgg aattttttat
     5341 aggtatcatc actttctctc acacccgatc tcctgctgta gaggaatgtt gtatcgttcg
     5401 cagcatcata tgcattgtac agtggtgtat acacccttgc attcacactg ctcatatcag
     5461 catatacggt tgatttgaat gtaaggttcc tgtgcaaagt gaattctgca aaggcgcttg
     5521 cgacgatcct gttttcctgg ctgatcgttt tgttatattc attttccagt atcaacagcg
     5581 ggttaattac acctgagttc tgtatgatcg gcaactcata gtacaggttg taattattgc
     5641 tgtctaatcc gtatggattt tttgtaaaga cagatttagt gcctgcacta acctgtggta
     5701 tcactttcct tgcagcatcc aatacccagg acgctccgta cgggttatcc tgtttaacag
     5761 tattgatatt aaatccaagt ttcaggaatt tatttacttt caactcatca gtaatagtta
     5821 actgcaattt tttcagcttt tcatgtttga tgataccttc atcggtggta taaccaactc
     5881 ccatgtaaaa cctgttcttt tcagtactgg tggcaatact caggttattg gtattgaatt
     5941 taccggttct tgttaccgca tccacccaat ctgtattggc agtccattta ccataatcaa
     6001 atggagaatt ggcacctata tttacttttt cttcttcgta caaagtctgg aattcacttg
     6061 cattggccca ggcaatttta tccaccagct ttttaaaacc aaatgcggta ttgaaattta
     6121 taaccgtctg accagctttc ccttttttag tagtaacagc gataacaccg ttagcaccac
     6181 gaacaccgaa gatggccagt gaagaagggt ctttcaatac ttctatggat tcaatatctg
     6241 aaggattgat ataatcaata ttatcattga agataccatc cactacatac agaggttgca
     6301 ggttgccacc caggctgttt gtaccacgga tacgaatatc cggagcagcg ccgggagtac
     6361 cattgttaat aatagtcaaa ccagctaccc tggcctgtaa acttgataca gggtttgtgt
     6421 taggtttatc agcaatgtct tttccggcta ctttcacaat agaaccggtc aggtctcttt
     6481 tgctggcagt tccatatcca atcaccacca cttcatcaat tttacgggaa gattgtagca
     6541 tggaaacatt caatgttgat tttccggcaa ccgccatttc aacggattca tagcccactg
     6601 aactaaatac caatactgca tcatccggaa ctgtgagtga aaaattacca ttagcatctg
     6661 ttgtagttcc ggttctggaa cctttaacgg aaacggacac ccctactaaa ggctcaccgt
     6721 tgtcccccgt tacttttccg gaaaccctca gatcctgaga agcaatctct ccggaagcaa
     6781 taccttcttt gagtactacc agttttgagc ccagtatttt atagctcact ccggtattcg
     6841 ccaggatctt gtcgagcaag tctgtaacag aagcatccac agcatctatg gttactttag
     6901 gttttctttt aaatgcgctt tcattgaaaa gaaaccggta attggttttc ttttcaatag
     6961 cgaataaaac ttttttaatt tccgcctcgt tcattttcag cgtaatacga tcctgcgaaa
     7021 agccgcgggc agaaacctgc agacaagcaa tcagcaaaaa agcaaacgtc aatttcataa
     7081 ttctggcaat cttttgataa aggtgatcct ttttagaaac agcagtcgtt tcgttttttt
     7141 tcatatttta caattgaagg ggttaataat gcggattccg gaaggaatcc actccttaac
     7201 atgttaacct ggcggggttt cgttacagcg aaatcccgca tttttttcat tatctaagaa
     7261 gtattgttca aagcaatcat tggcataagc attagcggtg tataattatt ttgtttcctg
     7321 ttttttcata ctgaaacgga atagtctctt ttaaagcatc cagtgcctgg gaaattgtct
     7381 ctgtttcgaa aataccattc agtctttctt cagcgatact ttcatccgtg atatcaatcg
     7441 ccacatcgta ccatctttcc attctgacag ccagttctgt aaaggactcg ttcctgaata
     7501 ccagtttatt attcacccat tgtgtttctg ctaccgtact gtccacagga ctgtatttga
     7561 gtttgtttac agaaatcagt gtactgatta ccggtaagga tgttctttca atattaccgg
     7621 tacgcatctc ccttacatca gcttttggaa cattattctc tacaaccagt ttttcgctgg
     7681 gcgaaagaat gatcttgtca ttgggcctgc ttcttatcgt tacctcaata ctgccacgga
     7741 tcaggcttgc ttctgtttgt ttatcgtcag gataggcttt tacgttgaac gcagtaccca
     7801 gcactttaat actaattgtg gtggtattga ttacaaatgg tttttcttta ttttttgcaa
     7861 catcaaaaaa accttcgccg gtcaatgtca cttcccttat ctctttcccg aaatctttat
     7921 tgtaggttag tttgcttccg gcattcagcc aaacaacaga gccatccggt aactgcactt
     7981 tggattttga gcccggacgg gtagttattt catttacctg ctgcgcaatc ggatttctct
     8041 ccggctcatc attaaacaaa ccaccaaatg caaaaaggcc aaccaaagca attactgctg
     8101 cagcagccca ataccatttt gtaaccagcc tgtttctttc ctttgcgggt atttctgctt
     8161 caattgccac ctccccgaaa ggtatattca attcagccat gcggtgaaga tgaagcatat
     8221 aggcatcttc ttcctgtaca aaatctttgt acggctggta tttccaaaga tcttccaggt
     8281 tctgtatggc atattgccat tcaggatgct gttgaatgag attttccagt aatcccattt
     8341 cctctgctga ggcttcccct gaaagttttt ttgaaaatag tacccaaaat agctcctggc
     8401 tcatgaatcg ttggttggtg aaagaagtaa gacaaccaaa acctctgctt acccctaaaa
     8461 gattttaact tttttttaca ttatttgcgt caggagataa ctggttgact tccatatgca
     8521 tatatttccc aatccggcgt aaagcaatgg ccatttgagc ttcgatagtt tttatggaga
     8581 gctgtaataa ctctgctact tctttatact taagcccgtc ttctttgacc agtttaaata
     8641 ttaaccggca gcggggaggg agattattga cggcctgcct gatctggcgc atcatttctt
     8701 ctgtcatcat caattgctca ggatcaaaat aaatactgtt cagctgcacc agccattctt
     8761 cgggacggat attttgctgc cttttttgct tattcaggcg attaaatgca agatttttgg
     8821 cagtagcata aaaatataaa aggggagaat ctatggtagt aagctgatct ctcttttccc
     8881 atactttgat aaaaaggtca gagaccagtt cttccgcttc ttcctgggat ttcagaatag
     8941 aataggcaaa ctgctttaag cgaccatgca aaaggaggaa taactcctta taggcagcct
     9001 ggtcgttagt aaaagcaata gcagatagca atctttttat cggctcattc tcaagcatag
     9061 caaagcgacg taaatctaag gattacattt tatatagcaa cccacttgtt tacaaggctt
     9121 tttttatact tttgcagccg ccttaccggc aggaccctta gctcagacgg ttagagcatc
     9181 tgactcataa tcagagggtc gttggttcga tcccaacagg gtccacaaat gctccgnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt tctttttgca caatagtttc
     9361 agcccgccag tattttttac ctttgcttat ccctcgcagc gccgggggtg tttttattat
     9421 tatgctggac aaaaggaatg ttatacaaaa cgaagagaaa gccgtgctgg taggagtggt
     9481 tctaaaagac cagacggaca cccaggtgca ggaatacctg gatgaactgg ctttcctggc
     9541 cgaaacagcc ggggcggtta ccgtaaaacg ctttacccaa aaactacaac acccggatag
     9601 cagaacattt gtgggaaaag gcaaactgga agaaattaaa aaatatgtac agggaaaaga
     9661 tatccgtatc gtcatttttg atgatgaact cagtggtgca cagatcaata atattgaaaa
     9721 agcactggat gtaaaagtaa ttgaccgcag tgacctgatc ctcgacatct ttgcccgccg
     9781 ggcaaaaacc gcacaggcca aagcacaggt agaactggcg caataccaat acatattacc
     9841 aaggctgaga ggtatgtgga aacaccttga acgtttgggt ggtggtattg gtaccagagg
     9901 accgggggaa acggaaattg aaacagaccg ccggattgta agagataaaa tttcattact
     9961 ccgtaaacga ttagcggaaa ttgataagca ggcatacaca caacgaaaag accggggtga
    10021 atttatccgg gtggcattgg taggttatac caacgtgggc aaatccacca ttatgaattt
    10081 gctgagcaaa cgggatgtgt tcgcagaaaa taaactattt gccaccctcg atactacaac
    10141 gagtaaagtt gtgtttgata caacgccgtt cttattaagc gacaccgtag gatttatccg
    10201 taagctgccg caccacctgg tagaaagttt taaaagtaca ttggatgaag ttcgtgaagc
    10261 tgatatacta ctgcatgtag tggatatatc acatccgaac tatgaagagc agataggagt
    10321 agtcaataaa acattgcagg agctcaacgc ttttgaaaag ccaaccctca ccatcttcaa
    10381 taaaatggat ctttacgaaa agcaaacctt tgatgaatgg ctggaggaaa gcaccaaaaa
    10441 agaaatcctg gaagacctgt atgaaagatg gcaacgggaa acaaacggcc atgcagtatt
    10501 tgtagcagcc attgaaaaaa gaaatttaga gacacttcgc aaaacgatac tggaaaaagt
    10561 aagagagttg taccggatac ggtacccgta taaaacagaa tttctgtatt aacctgtgac
    10621 agaaaaaaaa tacacctggt ataaaattgc cggtcatatc aacgaactgg attttgctga
    10681 taacaatatt gctgtaacag aacttaaggg taaaaagata tgcattggca ggcatcagga
    10741 agctgtcttt gcttttgcgt ataaatgtcc gcatgcagga ggaatattag ccgatggtca
    10801 tatagatgca ctgggtaata tagtatgccc tcttcatcgc tataagttca atatgcaaaa
    10861 tggccgcaat gtaagtggcg aaggctatt
//
LOCUS       DAORTI010000118        10784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_6377_length_10784_cov_6.091714, whole genome shotgun sequence.
ACCESSION   DAORTI010000118 DAORTI010000000
VERSION     DAORTI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_10784_cov_6.091714"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLO70_13025"
     CDS             complement(<1..781)
                     /locus_tag="PLO70_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQZ75435.1"
                     /translation="MFKKILIANRGEIALRVIRTAREMGIKTVAVYSTADRDSLHVKF
                     ADEAVCIGRPQSSDSYLNIPHLMAAAEITNADAIHPGYGFLAENARFAQICNDHGIKF
                     IGPTPDMINSMGDKITAKETMIKAGVPVVPGGQGLLQSVDEAKGIAGEMGYPVILKAT
                     AGGGGKGMRIVWEESELEKAYDTAKAEAAASFKNDGIYMEKFVEEPRHIEIQVAGDQY
                     GTVCHLSERDCSIQRRHQKLVEESPSPFMTDELRYRMGEAAK"
     gene            complement(840..1199)
                     /locus_tag="PLO70_13030"
     CDS             complement(840..1199)
                     /locus_tag="PLO70_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQZ75436.1"
                     /translation="MKKEVFDKNPILRLSFDFSLMVIEYCEQLEAQKRFVIARQLLKS
                     GTSIGANAMEAQNGESKADFIHKMKIAAKEAEESQYWLWLCDYAKNYPDCKPLILKLE
                     EINKVLGKILFTAKVPK"
     gene            complement(1298..1783)
                     /gene="accB"
                     /locus_tag="PLO70_13035"
     CDS             complement(1298..1783)
                     /gene="accB"
                     /locus_tag="PLO70_13035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQZ75437.1"
                     /translation="MDFKQIQELIKMVNKSNIGEVTIEQKDFRVTIKQKEDHVTQVVS
                     SAPVHAAPMQMAAPQPAASNQATAPAEKPKATEAPASNLLTIKSPMIGTFYRKSSPDK
                     PNLVDVGSEVATGTVVCIIEAMKLFNEIESEIKGKIVKVLVDDASPVEYDQPLFLVEP
                     A"
     gene            complement(1972..2538)
                     /gene="efp"
                     /locus_tag="PLO70_13040"
     CDS             complement(1972..2538)
                     /gene="efp"
                     /locus_tag="PLO70_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQZ75438.1"
                     /translation="MATTADISRGLIVKLDGSLYTVVEFGENKTARAAAKVWAKLKGV
                     DNNRSIEKTWNSGDNIYPVRVERKSYQFLYKDDTGYNFMDTETFEQVALQENMVDAPQ
                     FLKDGQEVSVLFNTETELPMSVELPDKIVVQITYTEPGLRGDTATRTLKQATVETGAT
                     VNVPLFVNEGELIRINTKTGEYVERVKE"
     gene            2676..5510
                     /locus_tag="PLO70_13045"
     CDS             2676..5510
                     /locus_tag="PLO70_13045"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQZ75439.1"
                     /translation="MRFRLHQLFLFILFSFFSYISEAQLIITPHPTIQALAQRLVGDG
                     VTISNVSFSGNSQMASFFLNRAGRTNIGIDSGIVLTSGRAKTVGAQFGVDGNGFAAAS
                     TVDADNGWNLPGDPDLANAIGQPVTELEDACILEFDFVPLGDSVRFNYVFSSEEYTPS
                     FVCDFNDAFAFFISGPGIMGLKNIALIPNTSIPVSIFNVNNVPGGTCPNNISYYKDNQ
                     ANTFFTHDGHTVVLTAREQVQPCETYHLKLVISDVGDDLFDSGVFLQARSLTSNTFGI
                     TNLTQTDPSNGDSYLVEGCAVGAFNITRPRKDPFPLNITLSYGGSAINSIDVQTLPSS
                     VIIPANDSLVTVNVIPLMDGLPEGIEQLKIFALAGCAAGTPTDSTMIQLRDYDILSLV
                     PDTAVICKGSSIQLIASAGYSTYQWLPDPTLSNTTIRNPIATPVSNNTTYVCTATEGT
                     CNARDSALIRWKYAQLLSKTDVNCGNASTGTIRVATGGEWQAPVQFSIDGINWQADSN
                     FINLPAGTYTVKIRDAGCTDSIVVTIAQAFPDLVISNTIITAAGCSGAADGQVSLTAS
                     GGNAPYSYSLDGTNFQANNSFNLGTGNFTITVKDINNCITSQPVVIPLNNTVTVNAGT
                     DTAICEGTVYLVPATSNATAFAWSPAATLQNANSLTPLASPVTATMYYVTATDGICTK
                     TDSIFINVWPAPLPDAGNDVAVCFGKVFELNGNGGVSYLWSPSTYLTTAANIASPSVK
                     ATNTISYFLQVKDSRGCNSLVPDEVIVTVTPAVQLFAGNDTIAATNQPIQLAAIELNN
                     SGVTQYNWSPATFLNNPSIANPVAILPFDMRYTVTGITPEGCEGMDELFIKVYKGPEI
                     YVPSGFTPDDNGLNDILRAIPVGIREFRYFRIFNRWGQLVFQTQDPAKGWDGKINGVK
                     QPTGTFVWMAEAIDYTGNRLNRKGVVTIIR"
     gene            5542..6042
                     /locus_tag="PLO70_13050"
     CDS             5542..6042
                     /locus_tag="PLO70_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQZ75440.1"
                     /translation="MKKFMFLLLNLLIIQSAIAQTDSLQPPYKRFPTYPPVKLLLPDS
                     TSFFSKADLPKKTAIMLMLFNPECEHCKQATEEMISKIDRFKNIRIVMATNTTFSRML
                     AFREKYQLAKYKNIIVGQDIHYFLPTFYMIRNLPFLAFYNRKKELISVFEGSLPMEKA
                     LLEFEK"
     gene            6123..7052
                     /gene="mdh"
                     /locus_tag="PLO70_13055"
     CDS             6123..7052
                     /gene="mdh"
                     /locus_tag="PLO70_13055"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQZ75441.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELCTELVLLDIKEGIAEGKAQD
                     MMQTATLLGFDTKITGSTNDYSKTAGSDVVVITSGLPRKPGMTREELIGVNAGIVKGV
                     CENILKHSPNAIIIVISNPMDTMTYLALQSTGLPKNRIIGMGGTLDSSRFKYQLSQHL
                     GCSPSDLNAVVVGGHGDTTMIPLIRLATWNSVPVTNFLSAEQQEKIVKDTMVGGATLT
                     ALIGTSAWYAPGAAGAALVESIVRDEKKLFTCCVALDGEYGQKDICLGVPVVIGKNGW
                     EKIVDFKLNEAEQAAFNKSADAVRSMNEVLKTL"
     gene            complement(7130..9784)
                     /locus_tag="PLO70_13060"
     CDS             complement(7130..9784)
                     /locus_tag="PLO70_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75442.1"
                     /translation="MKRACLFLSILLISTGLLNSQTGTGRNFYEISREQNEYFNRVGK
                     DKPGYKQFKRWEWYYSTRLGGNGQLVDNHQLNQEAVRRMSLSGAGSTGLTNVTTGSWS
                     ALGPGFVNSADKGIGRANRIAFHPTNPNILYVAGATGGLWISSDAGANWFSYSEGIPN
                     MSLSGVAVDYTNPNIIYILTGDADAAFSGARGQFAYGKTSTGVLKSYDGGFTWHQTGL
                     QWGETGTGSYLGFKLMMHPTNPQLLLVATNDGIFRTTDGGNTWTQSNNGQVVYDMEFQ
                     PSNSSVVYASATASDSIRVFKSTNSGQTFTATHAIDRVDDADGNNAANRSALAVSPAN
                     SSYVYLLTGPATANGEFHGVFRSVDAGESFTLRTNTPNILGGSSTGSDNNHQERYDLA
                     IAVSPTNISQVACGGIRLWTSTNGGSSFTWQDDNASASSYYHPDIHDLTYHPLNSGIL
                     YMCGDGGIYRSTDNGNNWTHVNNNLQLTQYYKIAINPNSGLGSENVMIGGTQDNGTNK
                     RSTGGGGTFSQILGGDGMDCYIDPDNASRYVASVQNGKFYYSTDAGSSFELVCDESSL
                     SATLGITVNSNWVTPVSEITGNSTQFVMGYQPAVLATRINTGVYAFTRIGWSGRSFVK
                     TARGNANRIYVGDNTYGGSQQNLVKTSTDMGSNWSDVYDANHAGRPVTDLAFAPNNGS
                     RMWLTFGGYNATNKVLYSGNGGDNGGSWIDITGSLPNVPVNCIVYDPNGTSIDAIYIG
                     TDIGVFYRDNNLGDWIPFSNGLPVVEITDLEIHSTEGLLRAGTYGRGIWETSLYSSCP
                     ANINLNTSNTSIYMPYYFQASQSISSTALHFGAGANVYYKAGTELILSPGFIGSAQGG
                     NTFEAAVGPCGGGVPNRSAQRKRGVLID"
     gene            complement(9813..>10784)
                     /locus_tag="PLO70_13065"
     CDS             complement(9813..>10784)
                     /locus_tag="PLO70_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75443.1"
                     /translation="DISINDPYGTLFFQVSSTARASIAMGGINDDLSIGTLAFNDLGK
                     MYFKTQSVNRMTIEPNGDVGVGFTSPSYKLDVNGNTRTVGNFIAEDGYIQIKNTTDSK
                     LWNMQYSSGTDRLLFLESGVERVILQNGGNVGIGETFPLEKLHVNGNTMIDGDATLNT
                     VNPTIQLQNSDVNKGFIQLSGDNIRIGTNSGNTAGKFVIRTNGGDRVFVDESGNVNIG
                     SQTDAPGYKLRVDGKMICEEVKVKLSTAWPDYVFDDKHKLMTIGELSKFIRQNKHLPN
                     IPSAKEIESNGMEVGDMQKRMMEKIEELTLYIISLQQQIDNLKKESR"
BASE COUNT         2942 a         2513 c         2143 g         3186 t
ORIGIN      
        1 tcttggccgc ctctcccatt ctgtaacgca gttcatccgt cataaacgga gaaggtgatt
       61 cttctaccag cttctggtga cggcgctgta tggagcaatc tctttcgctg agatgacata
      121 ctgttccata ttggtcaccg gcaacctgta tttcgatatg gcggggctct tctacaaatt
      181 tttccatgta gatgccatca ttcttaaaag aagcggctgc ttctgctttg gccgtatcat
      241 acgctttttc cagttcgctt tcttcccata caatacgcat acccttgcca ccaccaccgg
      301 cagttgcttt taagataaca gggtatccca tctccccggc aatccctttc gcttcatcca
      361 cactctgcaa taatccctgt ccgccgggta ccaccggcac accggctttg atcatggttt
      421 ctttggcagt gatcttatct cccatcgaat tgatcatatc aggcgtaggg ccgataaact
      481 ttataccatg atcattacag atctgtgcaa accgggcatt ttcggccagg aaaccatacc
      541 ccggatgaat agcatctgca ttggtaattt cagcagcggc catcaggtgc ggaatattaa
      601 ggtatgaatc gctgctctgc gggcggccga tacaaacagc ttcatcagca aattttacat
      661 ggaggctgtc cctgtcggca gtggaataaa cagccaccgt tttaataccc atttcccggg
      721 cagtacgaat aacccttaaa gctatctcac cacgatttgc tatcagtatt tttttgaaca
      781 tctggttaat ttaataattt gaaaatagct caattgctca ataccttaat ctacaactgc
      841 tattttggta cttttgctgt aaatagaatt ttacctaata ctttatttat ttcttccaat
      901 tttaaaataa gcggtttaca atcaggataa ttttttgcat aatcacataa ccaaagccag
      961 tactgtgatt cttctgcctc ttttgctgca attttcattt tatggataaa atcagcttta
     1021 ctttcaccat tttgcgcttc catggcattt gcccctattg aagttccgga ctttaataat
     1081 tgccttgcaa tgacaaatct tttttgcgcc tccagttgtt cgcaatactc gattaccatc
     1141 aatgaaaaat caaatgacaa tcttagaatc ggatttttat caaatacttc cttcttcata
     1201 tagcccatat ttggtttata aaacagagga atctgaattg aggaattgag ggattaagga
     1261 attgagcaat tatgaaactg gcttattatt ccggttatta agcaggttct actaagaata
     1321 acggttgatc atattcaacc ggcgatgcat catccaccag cacttttacg atctttcctt
     1381 ttatttcact ttcaatttca ttgaagagct tcatggcttc aataatacaa accacggttc
     1441 cggtggccac ttctgaaccc acatctacca ggttgggttt atcaggcgat gatttgcgat
     1501 agaaagtgcc gatcatcggg ctcttaatag taagtaaatt ggaagcaggt gcttcagttg
     1561 cttttggctt ttctgccggg gccgttgcct ggttgctggc tgcaggttgc ggtgctgcca
     1621 tttgcattgg cgcagcatga acaggtgctg aagaaacaac ctgtgttaca tgatcctctt
     1681 tttgtttgat cgtaacacgg aaatcctttt gctcaattgt tacttcgcca atattcgatt
     1741 tgttgaccat tttgatcaac tcctgaattt gcttaaagtc catgtgtttc aatttagtag
     1801 atgttttggt tttagaaagc cttaccctgt tggaaaggga ggctaaggta aggaaatcac
     1861 attgagaaaa ggcatgattt tttgctgttt ttgggcaaaa atgggtcgaa atgatacaaa
     1921 aatggcctga aacggtaaat ttctggccat tttctatcta aaatcatttg cttattcttt
     1981 cactctttct acatattcac ccgtcttggt gttgatacgg atgagctcac cttcatttac
     2041 aaacaatggt acattcaccg tagcacctgt ttcaacagtg gcctgcttca gtgtacgggt
     2101 tgctgtatca cctctcaaac caggttctgt ataagtgatc tgtacaacaa ttttatcggg
     2161 caactccaca ctcataggca gttctgtttc tgtattaaag agaacactta cttcctggcc
     2221 atccttcaga aactggggcg catccaccat attttcctgc aaggctacct gttcaaatgt
     2281 ctcggtatcc atgaaattat aaccggtatc atccttatac aggaattggt aactttttct
     2341 ttctacccgt acaggatata tattatcacc tgagttccag gttttttcaa tactgcggtt
     2401 attatcaacc ccttttaatt ttgcccacac tttagcggcg gcacgggctg ttttattttc
     2461 tccaaattca accacggtgt ataaactgcc atccagttta acgattagtc ctctgctgat
     2521 atctgctgtt gttgccatta gtatttcttt ttgaggccgc aaagataagg gagggccgtc
     2581 tctcagcaaa aggaagctta taagaagcct gtattgtatt agtggcaatt cattccggcc
     2641 ccattgtttt atattcgcaa aaggccaaaa gaattatgag atttcgactg caccaacttt
     2701 tcctttttat tctattttct ttcttcagtt atatttctga ggcccagttg attattacac
     2761 ctcatccaac tatacaggca ttggcacaac ggttggttgg cgatggcgtc acgatcagca
     2821 atgttagttt ttccggtaat agccagatgg cctccttttt tctaaaccgg gccggccgta
     2881 ctaatattgg tatagacagt ggtattgttt taacaagtgg acgggctaaa acagtgggcg
     2941 cccagtttgg tgtggatggc aatggattcg ctgccgcttc aacagttgat gctgataatg
     3001 gatggaacct gcctggtgat cctgaccttg ccaatgcgat aggccagcct gtaacagaac
     3061 tggaagatgc ctgtatcctt gaatttgatt ttgttcccct tggagacagt gtccggttca
     3121 actatgtttt tagttcggaa gaatacactc cttcctttgt ttgtgatttc aatgatgcat
     3181 ttgccttttt tatttccggg ccgggcatta tgggtttgaa gaatattgcc ctgataccca
     3241 atacaagtat accggtttcc atttttaatg taaacaatgt accgggcggc acttgtccaa
     3301 ataatatttc ttattataaa gacaaccagg caaatacttt tttcacccat gacgggcata
     3361 ccgtggtact aaccgcaaga gaacaggtac aaccttgtga aacctatcat cttaaactgg
     3421 tcatttcaga tgtgggagat gacctgttcg attcaggtgt tttcctgcag gcaagaagcc
     3481 ttacatcaaa cacttttggc attaccaacc tgactcaaac tgaccccagc aatggtgaca
     3541 gttacctggt agaaggatgt gcagtaggtg cttttaatat aacaaggccc cgaaaagatc
     3601 cttttccgct aaatattact ttatcgtatg gaggttccgc catcaactct attgatgtgc
     3661 aaaccttgcc atcaagtgtt attattcctg ccaatgacag tttagttacc gtcaatgtca
     3721 ttcctttgat ggatgggctc ccggaaggta ttgagcaatt aaaaatattt gccctggctg
     3781 gttgtgcagc gggtacaccc acagatagca ctatgattca gcttagggat tatgatatac
     3841 tttcccttgt gccagatacg gcggtaattt gtaaaggcag ttcaattcaa ttgatcgcct
     3901 ctgcaggtta tagcacttat caatggttac cggatcccac attaagcaac acaactatcc
     3961 gtaacccgat agccacccct gtcagtaata acacaaccta tgtgtgcacg gccactgaag
     4021 gtacctgcaa tgcaagagac tctgctctca ttcgctggaa atatgcacaa ctattatcta
     4081 aaactgatgt caattgcggc aatgcctcca ccggtacaat ccgggtagca acgggtggtg
     4141 aatggcaggc accggttcaa ttctctattg acggaataaa ctggcaggcc gacagcaatt
     4201 ttatcaacct accggccggt acctatactg taaaaatccg cgatgccggc tgcactgaca
     4261 gcattgtcgt tacaattgcc caggcattcc ccgatcttgt tatcagcaat actatcatca
     4321 cagcagcggg ttgctccggt gccgcagacg ggcaagtcag tttaacggcc agtggtggta
     4381 atgcacccta ttcttactca ctggatggta ctaatttcca ggcaaacaac agcttcaacc
     4441 ttggcacggg aaatttcaca ataactgtta aggacatcaa taactgcatc acttcacagc
     4501 cggttgttat cccgttaaat aatacggtca cagtaaatgc aggtacagat actgctattt
     4561 gtgagggtac ggtttacctg gttccagcca cgtccaatgc aactgcattt gcgtggtcac
     4621 ccgccgcaac cttgcaaaat gcaaacagct taactccact tgcatcacct gtcactgcaa
     4681 ccatgtacta tgtaacggcc acagatggca tttgcacaaa aacagattca atatttataa
     4741 atgtatggcc tgcaccttta ccggacgccg gtaatgatgt ggcagtttgt tttggtaaag
     4801 tttttgagct gaacgggaat ggtggtgttt cttatctatg gtcgccgtca acctacctga
     4861 caactgctgc caatatagct tctccttcag taaaagccac gaataccatt tcttatttcc
     4921 tgcaggtaaa agatagcagg ggttgcaact cacttgtacc agacgaagta atagtaacgg
     4981 taaccccggc cgttcagctt ttcgccggca atgatacaat tgctgccacc aaccaaccca
     5041 tacagcttgc tgctattgaa ttaaacaatt cgggagttac gcaatacaac tggtcacccg
     5101 ctactttcct caacaatcct tctatcgcaa acccggtagc cattcttccg tttgatatgc
     5161 ggtataccgt tactggtatc actcccgagg gttgcgaagg catggatgaa ctttttatca
     5221 aagtatataa aggcccggag atctatgtac cctccggctt taccccggat gataatggat
     5281 taaatgatat ccttcgggcc attcctgtcg gcatccggga gtttcgttat ttccgaattt
     5341 ttaatcgttg gggccaattg gtattccaga cacaggaccc tgccaaagga tgggatggaa
     5401 aaattaacgg ggtaaaacaa cctaccggca catttgtatg gatggctgaa gctattgact
     5461 atacaggcaa ccgcctcaac aggaaaggtg tagttactat catccgctaa caaaggactg
     5521 aacagcactt aactttacgg catgaaaaaa ttcatgtttc tcttactgaa cctgctgatc
     5581 attcaatcag ccattgcaca aactgacagt ttacaaccac cctataaaag atttccaacc
     5641 tacccgccag taaaattatt gctgccagac agtaccagtt ttttcagcaa agccgacctg
     5701 ccaaaaaaaa cggcaatcat gctgatgctt ttcaacccgg aatgtgaaca ttgcaaacag
     5761 gcgacggaag aaatgatctc taaaattgac aggtttaaga atatccggat agtaatggct
     5821 accaatacaa cttttagcag gatgctggct ttccgggaaa aataccagct tgcgaagtat
     5881 aagaatatta ttgtaggaca ggacatccat tacttcctgc caacctttta tatgatccgt
     5941 aacctgcctt tcctcgcatt ttataaccgg aagaaagaac ttatttctgt ttttgaaggt
     6001 tccctgccga tggaaaaagc gttgctggaa tttgaaaaat aaaccgcttc atcacccgaa
     6061 aaacaaggtg gttccgctac ctttgcggcg aaaatatttt ctccatttat aaatttttat
     6121 tcatgaaagt aactgttgta ggcgctggtg cagtaggtgc aacctgtgct gataatattg
     6181 cccgtaaaga actttgtact gaactggttt tacttgatat caaggaagga attgcagaag
     6241 gtaaggcaca ggatatgatg cagactgcca ccttattggg ttttgacacc aagataaccg
     6301 gcagcaccaa tgattattca aaaacggccg gcagtgatgt ggtggttatt acttccggtt
     6361 tgccccgcaa acccggtatg acccgtgagg aactgatcgg tgtgaatgcg ggtattgtaa
     6421 aaggtgtttg tgagaacata ttaaagcatt ctcccaatgc aatcatcatt gtgatcagca
     6481 acccgatgga tacgatgaca tacttagcgt tgcaatcaac cggtttaccc aagaacagaa
     6541 ttatcggaat gggcggtaca ctcgacagca gccgttttaa ataccagttg agccagcatt
     6601 taggttgcag cccttcagac cttaatgccg tggtagttgg cggacatggt gatacaacga
     6661 tgatcccgct gatacgcctg gctacatgga acagtgttcc tgttactaat ttcttaagtg
     6721 cagaacaaca agaaaaaatc gtaaaagaca ccatggtggg tggtgctaca ttaacagctt
     6781 tgatcggcac atcggcctgg tatgctcccg gcgctgccgg agcagcattg gtagaatcca
     6841 ttgtaagaga tgagaaaaaa ttatttacct gctgtgtggc actggatggt gaatatggac
     6901 aaaaagatat ctgcctgggt gttcccgttg ttatcggcaa gaacggctgg gaaaagatcg
     6961 ttgactttaa actgaacgaa gccgaacaag ctgctttcaa taaaagtgca gatgcagtga
     7021 ggagtatgaa tgaggtgttg aagacattat aataaataaa ttcaatttta aaaaaaacgg
     7081 catggatata caatacatgc cgttttttta atataaacct atgcagtcat taatcaataa
     7141 gtacgcctct tttcctttgt gcagacctgt taggcactcc tccaccacat ggacctaccg
     7201 ctgcttcaaa cgtattacct ccttgtgcag aaccgataaa tccggggctt aaaatcaatt
     7261 ccgtacctgc cttataataa acatttgcac cggcaccaaa atgcaatgca gtagaactaa
     7321 tactttgtga agcctggaaa tagtaaggca tataaatgga tgtattactt gtgttcaggt
     7381 taatatttgc aggacagctg ctgtataatg aggtttccca tattccacgt ccgtaagtac
     7441 ccgctcttaa taatccttct gtcgaatgta tttccagatc ggttatttcc accaccggca
     7501 atccgttgct gaaaggtatc caatcaccaa ggttattatc cctgtaaaac acacctatat
     7561 cggtaccgat gtaaatggca tcgatggatg taccattcgg atcatataca atacagttta
     7621 ccggtacatt gggaagtgat ccggttatat caatccaact gccaccatta tctcccccgt
     7681 tacctgaata caacacttta ttggttgcat tgtaaccgcc aaaagtcagc cacatcctgc
     7741 ttccattgtt tggagcaaag gccagatcag ttaccggcct tccggcatgg ttagcatcat
     7801 acacatcact ccagttggac cccatatcgg tgcttgtttt aaccaggttt tgctgactac
     7861 ccccataagt attgtcacca acataaatcc ggttggcatt accccttgct gttttaacaa
     7921 aacttcggcc ggaccaacca attcttgtaa acgcatacac accggtatta atcctggtgg
     7981 ccagtacagc aggctggtaa cccattacaa attgggtaga gttacctgta atttctgaaa
     8041 caggtgttac ccagttggaa tttacagtaa taccaagtgt agcagacaaa ctgctttcgt
     8101 cacaaactaa ttcaaacgaa gaaccggcat ctgttgagta ataaaacttt ccgttctgca
     8161 cactggctac atacctgctt gcattatccg gatcaatgta gcaatccatt ccgtccccgc
     8221 ccagtatctg tgaaaaagta ccgcctccac ctgtacttct tttgttggtt ccattatcct
     8281 gtgtgccgcc gatcattaca ttttcagaac caaggccact gttggggttg atcgcaattt
     8341 tataatactg ggttaattgc aggttgttat taacatgcgt ccagttattc ccgttatctg
     8401 tactgcggta aattcctcca tcaccacaca tatacaaaat accactgttc aaagggtgat
     8461 aagtaagatc atgaatatcg ggatgataat aactcgatgc acttgcgtta tcatcctgcc
     8521 aggtaaaact gctgcctcca ttagtggatg tccataaccg tatgccacca caggccacct
     8581 ggctaatatt cgttggacta accgctatcg caagatcata gcgttcctgg tgattattat
     8641 ctgaacctgt tgaactaccg cctaaaatat ttggcgtatt agttcttagg gtaaatgatt
     8701 cgcctgcatc aacggaacgg aaaacaccat gaaactctcc atttgcagta gccggccctg
     8761 tcagtaaata cacataagaa ctattggcgg gacttactgc taatgccgat ctgttggcag
     8821 cattatttcc atcagcatca tccacccggt caatggcatg agtagcggta aacgtttgcc
     8881 cgctattggt ggatttaaat acccgtatac tgtctgaagc agtagccgaa gcatatacaa
     8941 cagaagaatt ggatggctga aactccatat cataaacaac ctgtccgtta ttcgactgag
     9001 tccatgtatt tcctccatcc gtggttctga agatgccatc gtttgtagct accagtaata
     9061 gttgagggtt ggtgggatgc atcattagtt taaacccaag ataactgccc gttcccgttt
     9121 caccccactg aagtcctgtt tgatgccagg tgaatcctcc gtcatacgat tttaaaacac
     9181 cagttgatgt ttttccatag gcaaattgtc ccctggcacc gctgaaagct gcatccgcat
     9241 cacccgttaa aatataaatg atattgggat tagtataatc caccgctacc ccactcagcg
     9301 acatattcgg aatgccttca ctgtatgaaa accagttggc accagcatct gaactgatcc
     9361 ataaaccacc agttgcacct gcaacatata atatatttgg attggtggga tgaaacgcaa
     9421 tcctgtttgc ccggccgatt cctttatctg ccgagtttac aaaaccggga cccagtgctg
     9481 accaactgcc ggtagtaaca ttggttagtc ccgtagaacc agcaccgctc agggacatac
     9541 gccgaacagc ctcctggttc aactgatgat tatctactaa ctgcccgttc ccgcccaggc
     9601 gggtggaata ataccattcc cagcgtttga attgtttata accgggcttg tccttgccaa
     9661 ccctgttaaa atattcgttc tgctcccggc tgatctcata aaaatttcta cctgttccag
     9721 tttgtgaatt aagcaggccg gtgcttatga gtagtatgct taaaaaaaga caagctcttt
     9781 tcataaatta tttacttaaa ataaactgac cactacctgg attccttttt caggttatcg
     9841 atctgttgct gcaaactgat gatgtagaga gtaagctctt caatcttctc catcatcctt
     9901 ttttgcatat cacccacctc cataccattg ctttctattt ctttagcaga agggatatta
     9961 ggcaaatgtt tattctgccg aataaacttc gataattcac caatggtcat cagcttatgc
    10021 ttatcatcaa acacataatc cggccaggcc gtacttagtt ttacttttac ttcttcacat
    10081 atcattttcc cgtctactct gagcttataa cctggtgcat ctgtttgaga accgatatta
    10141 acattgccac tttcatccac gaacacccgg tcacctccat tggtgcggat gacaaatttg
    10201 ccggctgtat ttccgctgtt ggtaccaatc cggatattat ctccggagag ttgtataaac
    10261 cctttattaa cgtcactgtt ctgcaactgt atagttgggt taacggtgtt taaagtcgca
    10321 tcaccatcaa tcatcgtgtt gccattaaca tgtaattttt ctaacgggaa agtttcaccg
    10381 atacccacat ttccgccatt ttgtaatatc acccgttcta cacctgactc caggaataat
    10441 aaacggtctg ttccactgga atactgcata ttccagagtt ttgaatcagt agtattcttt
    10501 atctggatat aaccgtcttc agcaataaaa tttcccaccg tccttgtatt accatttaca
    10561 tccagtttat aagaggggct tgtgaacccg acacccacat ccccattcgg ttcaatggtc
    10621 attctgttca cactttgcgt tttaaaatac attttcccca aatcattaaa ggcgagtgtg
    10681 ccgatactga gatcatcgtt gataccaccc attgcaatac tggccctggc agtactggaa
    10741 acctggaaaa aaagtgtacc ataaggatca ttaatactta tatc
//
LOCUS       DAORTI010000119        10144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7008_length_10144_cov_6.569531, whole genome shotgun sequence.
ACCESSION   DAORTI010000119 DAORTI010000000
VERSION     DAORTI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_10144_cov_6.569531"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1353
                     /locus_tag="PLO70_13070"
     CDS             397..1353
                     /locus_tag="PLO70_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ75444.1"
                     /translation="MELKKFLRQSTFAIALSVPVLITGCDKDDDPVNNTITDVVVGNA
                     SFTTLEAAVVKAGLQTTLSGTGPFTVFAPDDAAFTASGITSTVLNSLTPAQVSNILLY
                     HTLGAKVTAADVPAGPNAKVTTASGDSVFVTKNANGVFINGIKVNTADVAADNGVIHV
                     IGRVLNPPVGNIVETAVASGLDSLVKAVTRATTAPGGDPTLAGTLSTARLTVFAPTNA
                     AFTQLLGALSLTDINQIPVGTLLAVLRYHVVGGRAFSSDLTNGSLPMLAGGNTTTNLT
                     NGTGGGPSIQGNGNGGNRSNITATNIVCRNGVVHLIDRVLLP"
     gene            1444..2472
                     /locus_tag="PLO70_13075"
     CDS             1444..2472
                     /locus_tag="PLO70_13075"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ75445.1"
                     /translation="MFSHRYRYLFIVLLSLYTFMNTALCNVYYYFKIEIEWYFALLTI
                     VAVTLFTWEGNRLIKPYFRKKFLTPKTKIRFLAFFFLAGNIVALIAATVAVYLFGNLL
                     HGYTWEQNLNPLKLNIIYASLINLFLHLLNAIYFFFREYRRHWKEVEELRQTSTQAKI
                     QLVKSQINPHFLFNNLNVLSGMVIKDNPEANHFIEEFSKVYRYILNNQEKELVELRSE
                     LEFIQPYIFLLQKRFDNGLTVSVSIPDHYKMYHVVPAALQMLIENAIKHNVVSRSRPL
                     HIDIHANGNDTLIIKNNLQPRQAVEPSTRIGLQNIRKRYELISGRDVVVKKTERDFEV
                     TIPLLQLN"
     gene            2478..3230
                     /locus_tag="PLO70_13080"
     CDS             2478..3230
                     /locus_tag="PLO70_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ75446.1"
                     /translation="MKVIIVEDEKLATERLQTLLKTYDSSIEIIACLESIEDTVHYLK
                     NHSHPDLLLLDIHLSDGHSFEIFKQVSYNRPIIFTTAYDEYALDAFKMLSIDYILKPV
                     SGEALATALNKFKSMSLSFSATDLNRVSQGWKDQRYKKRFLGKIGQRLFFIDADEVAF
                     FQADNKIVYLADKEGNRYIIDHTMERLEEQLDPQHFFRLNRKFIVNISAIQQVKPYYN
                     NRLRLSVKGANQQEEMVISRERVADFKIWADA"
     gene            3286..3918
                     /locus_tag="PLO70_13085"
     CDS             3286..3918
                     /locus_tag="PLO70_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQZ75447.1"
                     /translation="MHYIPHLSKDKKLKKLVESGEPHQLKKRKNICTYLCASIMSQQL
                     SVKVADVIYKRFLNLYGGKEPTPQQILDTPFEQLRGIGLSNAKVNYVKNVAQFEIESG
                     MDAKKLGKMDNEEVITYLTQIKGVGRWTTEMLLMFALGREDVFAVDDLGIQNAMIKLY
                     KLDNNDKKKFRINLLKISEKWSPYRTYACLHLWQWKDKAPALKKTKEKIK"
     gene            3943..4581
                     /locus_tag="PLO70_13090"
     CDS             3943..4581
                     /locus_tag="PLO70_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQZ75448.1"
                     /translation="MRKLIAAAIICTFFSFSPCNHNSKDGWIPLFDGKSLDGWKVGAN
                     ANTFSIENGAIAVNGNVAHLFYNGEVQQHSFRNFEFRADVMTMPGSNSGIYFHTQYQE
                     SGWPQKGYEAQVNNSHTDWRRTGSLYAIQDVKEVYVKDGEWFTEQIIVQGKKITVKIN
                     DKTVVEYTEPGNPQREEGMKGRVLSTGTFALQGHDPKSKVLYKNIRVKVLPD"
     gene            complement(4610..4966)
                     /locus_tag="PLO70_13095"
     CDS             complement(4610..4966)
                     /locus_tag="PLO70_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQZ75449.1"
                     /translation="MLTTIHPKLPMRDKTATRNYYINQLGFQEFGSADFDGYLMVAKD
                     NIQIHFFEFKELDPKENYGQVYIRTDAIKELYQDLLNKKVAIHPNGQLTVKPWGQKEF
                     SLLDPDNNLLTFGQSL"
     gene            5228..6658
                     /locus_tag="PLO70_13100"
     CDS             5228..6658
                     /locus_tag="PLO70_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQZ75450.1"
                     /translation="MKIFILLLVYSCTMQVARSQNTGVGTPTPVEKLDVNGAIKIGNT
                     AGNNEGTIRYNNGNFEGRTTNLWQNLAVPLNAMVLSESSNDANLLANGFSLKGPLNVT
                     ENTLINATAAAGSWHPVSEYYSRVNTVSVTANSRIAIWGGVKDGVYLNTGAFYDPVND
                     LWESISTVGAPSPRIGHALVWNGTELVVWGGFTYNGSYAYLGDGAKYNSITKTWTPLN
                     SAGAPSARGNVTSGYNSGTNEMIVWGGFNGPTLNEGYRYNFTTDTWTPVSLVNSPGSR
                     INYAHATANGYFLVWGGYSFASSGQVNTGALYNFSTDTWTPTSLASAPAARENADAVW
                     NGTQMIVWGGIAGAVNQSGGARLNPVTNTWATMAAGPVADNGATVAYAGSFMLIYGIG
                     GGALYNNTANTWSSLTGNYPAAGRNGAVVQSANNVLVVWGGEQYGAVGDFPQYYNDGR
                     RYFWFARSITSTVPDIKTYYLYKKIM"
     gene            complement(6768..7631)
                     /locus_tag="PLO70_13105"
     CDS             complement(6768..7631)
                     /locus_tag="PLO70_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQZ75451.1"
                     /translation="MKKITWTFTQNNSDTILSDGLYKLKTRPYQLFSDIDHNGFGNYL
                     ISHNKTNYYVGEGKELSKRLKQQFKPTTSTFYKNLLKLQKTNKAIGNISIDAFKVQVI
                     VTDIGRKEIEEFGISNLPTLLNRFQLDKRKLHNVAHQDGLWDNVQKAKTDILLQAEQE
                     ILQGKFSSWFDCNAESVAGLYIVRDKHDKLIYIGESSDICERHKTHSSRTYFSALRRH
                     IGTELLNFELQERNGKKKYFEVSEDKKITEFLKSCKATFYPTHFGRYELEEFLIKKHS
                     PLLNRKDNKDE"
     gene            complement(7831..8649)
                     /locus_tag="PLO70_13110"
     CDS             complement(7831..8649)
                     /locus_tag="PLO70_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQZ75452.1"
                     /translation="MKKRFIILIDFSEYSRNLIKYACNWSKQVSAEFLLVHQSIVLAP
                     ALTDNESRQQIAQHTNEEALQKLKALAKEIIPPTIKVSFNVSESNLQLTLPKLLEDPY
                     ENLIFTGIKGTGLLKKLFLGSVALQVVDNTKNIIVAMPKEIDTFSHEKIFVAVTEKHP
                     LNILELNKFLNLIDSKNTSITFFYLAKPNEKTKGIEKHLRELCAMFVDRFNTDFAIYE
                     GNNPFEDIKKVINNKIDELLIVQKGSRLLTDQLFRRFLINELVYEGQTPMIVLP"
     gene            complement(8653..>10144)
                     /locus_tag="PLO70_13115"
     CDS             complement(8653..>10144)
                     /locus_tag="PLO70_13115"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HQZ75453.1"
                     /translation="RKKRMNLTIENIVLVGSLLLFVSIVVGKTSYKFGVPTLLLFLAI
                     GMLAGSDGIGGIHFDNPQIAQFVGIVSLNFILFSGGLDTNWTSVKPILREGLVLSTLG
                     VLLTALSLGTFVWYVTDFTIYESMLLGSIVSSTDAAAVFSILRSKSLALKTNLRPTLE
                     LESGSNDPMAYVLTIAFLTLVINQDQGITSIIPLFFQQMILGGIAGFAFGRLSKFIIN
                     KIKLDFEGLYPALVIALMFITFSATDFVGGNGFLAIYICAVYLGNQDLIHKKTILKMF
                     DGLAWLMQIVLFLTLGLLVFPSQIIPYFGIGILISLFLIVVARPVSVFLSMMFFKMRL
                     RRRFYISWVGLRGAVPIVFATYPLLAGIEKANMIFNIVFFISVTSVLIQGTTLSIVAK
                     WLNVALPENAKKITEIDKLILELPKSSLQEFEILPDFHSVNKRIVDLNFPKSAFIVMI
                     RRNGEYIRPGGSTEIEAQDILMVLADSQEDFTKVNESLQKPNTSKT"
BASE COUNT         3043 a         1962 c         2116 g         3023 t
ORIGIN      
        1 agattttgtc acctccgata agatcagaaa tcagctggcc cagttaggaa tcaccttaaa
       61 ggacgagaag ggtggcgaca tgagctggag tgtggaatag ggaggaggtt gtattcctga
      121 atgaattgaa gtaaagaggc tgtctgagaa gctccattcc atgtcatatt gagcaaagcc
      181 gaaatatgac atggaatggt aaataaattt gtacgcattt cgataaactc aatgtgacaa
      241 atttgtttgc ttctcagaca gcccttttaa ttttccctta aacaaactca ctccgccaaa
      301 acatcaactc actccccgaa actgacgatt cactcctgct ttgtttctct tttccactgg
      361 atcatgttaa tatgattaaa caaaaattat tttaacatgg aactcaaaaa atttcttcgt
      421 caatcaacct ttgctatagc attgagtgta cccgttctta ttacaggatg tgataaggat
      481 gatgatccgg taaacaatac catcactgat gtggtggtag gtaatgccag ttttaccacc
      541 ctggaagcag ctgtggtaaa agccggtttg caaacaactt taagtggtac tggccctttc
      601 actgtttttg caccggatga tgcagctttc actgcttcag gtattacgtc aactgtactc
      661 aacagcctta cacctgcaca ggtatccaat attttattgt accatacttt aggtgctaaa
      721 gtaactgctg cggatgtacc tgctggtcca aacgcaaaag tgaccactgc aagtggtgac
      781 tctgtatttg taaccaaaaa tgctaatggt gtcttcatca atggtattaa ggtaaataca
      841 gctgatgtgg ctgctgataa cggcgttatc catgtaattg gccgtgtatt aaatccacca
      901 gtaggaaata ttgtagagac agcagtagcc agcggtcttg attccctggt aaaagcagta
      961 acccgtgcta ccactgctcc cggtggtgac cccactctgg ccggaacatt aagtactgcc
     1021 aggctgactg tattcgctcc aaccaatgct gcctttacac aattattagg cgccttatcc
     1081 cttaccgata ttaaccagat tcctgtaggt actttgctgg cggttctccg ctaccatgta
     1141 gtgggcggaa gggctttctc ttctgacctt acaaatggtt cattgccaat gctggcaggt
     1201 ggaaatacga ctaccaacct gaccaacgga acaggtggtg gcccctctat ccagggaaat
     1261 ggtaatggtg gcaaccgttc taatatcacc gctacgaaca ttgtatgccg gaatggtgtt
     1321 gtacacctga ttgacagggt tttgttgcct taattataaa catagtcagt acctcctctg
     1381 aaaaatcaat gtctgctctg ttccctttaa accggaacag ggcagattta attaaaagca
     1441 tttatgttca gccatcgtta caggtacctt ttcattgtat tgctgtcgct gtacactttt
     1501 atgaatacag cgctatgcaa tgtctattac tatttcaaaa ttgaaataga atggtatttt
     1561 gcgttgctga cgattgtggc tgttacgctt tttacctggg aagggaacag gctgatcaaa
     1621 ccatatttca ggaaaaaatt tcttacccct aaaacaaaga tcaggttcct ggctttcttt
     1681 ttcctggcag ggaatattgt tgcacttatt gcggcaacgg ttgcagttta tttattcggc
     1741 aacctgcttc atggatacac ctgggagcag aacctgaacc cgctgaagct gaatattatt
     1801 tatgcaagcc tcatcaatct tttcttacat cttttaaatg ccatttattt ctttttccgg
     1861 gaataccgcc gtcactggaa agaagtggaa gaactgcgcc aaaccagtac acaggctaag
     1921 atacaattgg taaaaagcca gatcaatcct catttcctgt tcaataacct gaatgtattg
     1981 tccgggatgg tgataaaaga taacccggag gctaaccatt ttattgaaga gttctccaaa
     2041 gtgtaccgtt atatcttaaa caaccaggag aaggaattag tggagctccg ctccgaactg
     2101 gaattcatac agccatatat ttttttactg caaaaaagat ttgataacgg attgacggtg
     2161 agtgtgagta taccggacca ctataaaatg tatcatgtgg tgccggcagc tttgcaaatg
     2221 ctcattgaaa atgccatcaa acataatgta gtgagcagga gccgtcccct gcatattgat
     2281 atacatgcca atgggaatga cacgctgatc ataaagaaca atttgcagcc aagacaggct
     2341 gtagagccct ccactcgtat cgggctgcaa aatatccgca aaagatatga actaatcagc
     2401 ggcagggatg ttgtggtgaa gaagacggaa cgggattttg aagtaacaat tccccttctg
     2461 cagttaaact aacgggtatg aaagtaataa ttgtagaaga cgaaaagctc gccaccgaac
     2521 gtttgcagac cctgctcaaa acgtacgatt cttctattga aattatagcc tgccttgaaa
     2581 gtattgaaga caccgttcat tacctgaaaa atcatagtca tcctgacctg ttattgctgg
     2641 atattcattt atcagacggc catagttttg aaatattcaa gcaggtcagt tacaacaggc
     2701 ccattatttt taccactgct tacgatgaat atgcgctgga tgcattcaag atgctcagta
     2761 ttgactacat attgaaaccg gtaagcgggg aagccctggc aactgcactg aataagttca
     2821 aatcaatgtc tttgtctttt tctgccaccg atctgaaccg ggtaagccag ggttggaaag
     2881 accagcgcta caaaaaaaga tttttaggca agattggtca gcgtcttttt ttcattgatg
     2941 cagatgaggt agcttttttc caggcagata ataaaattgt gtacctcgcc gataaagaag
     3001 gcaaccgcta tattattgac cacactatgg agcggctgga agagcaatta gatccgcagc
     3061 attttttccg gctcaacagg aagtttattg tcaatatcag tgctatccag caggtaaagc
     3121 cttactacaa taacaggctc cggctttctg taaaaggagc taaccagcag gaagaaatgg
     3181 tgatcagccg ggaacgggtg gctgatttta agatctgggc agacgcctga aggaaaaatt
     3241 aaaggttaga cagttattat tatggatact gtttaacttc atttcatgca ttacattccc
     3301 catctttcca aagataaaaa actaaagaag ctggttgaaa gcggagaacc gcatcagtta
     3361 aaaaagcgga agaatatctg tacttatctc tgcgcttcca ttatgagcca gcaactgtct
     3421 gttaaagtag ccgatgtcat ttacaaacgc ttcctcaatt tgtatggagg caaagaaccc
     3481 acaccgcaac aaatactgga tacgccgttt gaacaattaa gagggatcgg gttgagcaat
     3541 gccaaagtga actatgtaaa gaatgtggcg cagtttgaaa ttgaatctgg catggatgca
     3601 aaaaaactgg ggaagatgga taatgaagaa gtgataacct atttaacgca gataaaagga
     3661 gtggggcgct ggacaacgga aatgttgctg atgtttgcgc tgggcaggga agatgtgttt
     3721 gctgtagacg acctgggcat acaaaatgcg atgatcaaat tgtataaact ggataacaac
     3781 gataaaaaga aattcaggat aaacctgctg aagatctccg agaaatggag cccctaccga
     3841 acctatgcct gcctgcattt gtggcaatgg aaggataagg caccggcgct gaaaaagacg
     3901 aaagaaaaaa ttaagtagct tcccttcctt aaagacatca ctatgcgaaa gcttattgcg
     3961 gcagccatta tttgtacttt tttttccttt tccccctgca atcataatag taaggacgga
     4021 tggattcctt tattcgatgg taaatcattg gatggctgga aggtgggtgc caatgccaat
     4081 actttttcga tagagaatgg cgccattgcc gtaaatggca atgtggccca tttattttat
     4141 aacggggaag tgcagcagca ttcgttcagg aattttgagt tcagggccga tgtgatgacg
     4201 atgccgggtt ccaattcagg catttacttt catacgcaat accaggaaag cggctggccg
     4261 cagaaaggat atgaagcaca agtaaacaat tcacatacag actggcgccg taccggcagc
     4321 ctgtatgcca tacaggatgt aaaggaagta tatgtaaaag acggggaatg gtttactgaa
     4381 cagatcatcg tacaaggcaa aaaaataacg gtaaagatca acgataaaac agtggtagaa
     4441 tataccgaac caggcaatcc gcagcgggaa gaaggaatga aggggcgggt attatctaca
     4501 ggcacttttg ctttgcaggg ccacgacccg aagagtaaag tgttgtataa aaatatcagg
     4561 gtgaaagtat taccggatta gcataggtca ttaccatcgc tgcaactttt cacaaacttt
     4621 gtccgaaggt caataagtta ttgtccgggt caagtaaaga aaattccttt tgtccccacg
     4681 gttttacagt taattgtcca tttggatgaa tagcaacttt cttatttaat aagtcctggt
     4741 ataattcttt aatggcatct gttctgatat acacctgtcc gtagttttct ttgggatcca
     4801 attctttgaa ttcaaaaaaa tgaatttgaa tattgtcttt agctaccatc aggtaaccgt
     4861 caaagtcagc actgccaaat tcctggaaac ctaactggtt aatataataa ttccgggttg
     4921 cagttttgtc ccgcatcggc aactttggat gaatagtggt aagcataatt cccggattga
     4981 ttttatagtc tacttgtttt aaaagagaca atggttacag ccgcacaaca aatttatcag
     5041 ttttgaagta ccggagtgta gcatttgtat aaacggtcat atgaaatgtt tcttccattt
     5101 gttgagtgta tgcgggaaag tacagatggt tcaacatact ttactgcgta aaagtcttat
     5161 agtgatcata tagctcatat ggtcaaataa gcttttaaaa aagttctgta tttttaagaa
     5221 aaacactatg aaaattttta tactgttgct ggtttatagc tgcaccatgc aggtggcccg
     5281 gtcacaaaac accggcgtgg gcacacccac accggttgaa aaactggatg ttaacggagc
     5341 tattaaaatt ggtaatacgg ccggtaataa tgaaggcacc atacggtata acaatgggaa
     5401 ttttgaaggc cgtactacca atctgtggca gaacctggca gtgcccctga atgcgatggt
     5461 tttatctgaa agcagtaatg atgctaactt attggcaaac gggttttcat taaagggccc
     5521 gctgaatgta acggaaaaca cactgatcaa tgctactgca gcggctggaa gctggcatcc
     5581 tgtatcggaa tactatagca gggtaaatac agtttccgtt acagcaaact cccgtattgc
     5641 tatatggggc ggagtaaaag atggagtgta tttaaacacc ggtgcatttt atgatccggt
     5701 aaatgacttg tgggaatcca tttctacagt aggcgcacca agtccccgga taggccatgc
     5761 actggtttgg aacggtactg agctggtagt gtggggtggg tttacatata atggcagtta
     5821 tgcttatttg ggagatggtg caaaatataa ttccataacg aaaacatgga ccccgttaaa
     5881 ttctgccggg gcacccagtg ccaggggaaa tgtaaccagc ggctacaata gtggcaccaa
     5941 tgaaatgatc gtttggggag gttttaatgg ccccactctt aatgagggat accgctataa
     6001 ttttactacc gacacctgga cgccggtaag ccttgttaat tctccgggta gccggatcaa
     6061 ttatgcacat gctacagcta acggctattt cctggtgtgg ggtggatata gttttgccag
     6121 ttccggccag gtaaacacag gcgcccttta taatttttcc actgatacct ggacacctac
     6181 ttctttggca agtgcacctg ctgcaagaga aaatgcagat gctgtatgga acggaactca
     6241 aatgattgtg tggggtggaa ttgcgggagc agtcaaccag tcgggtggtg ccaggcttaa
     6301 ccctgttacc aatacatggg caaccatggc tgcgggacca gttgctgaca atggtgctac
     6361 tgttgcctac gcaggttcct ttatgctgat ctatggtatt ggtgggggtg cattgtataa
     6421 taatactgcc aatacatgga gcagcctcac gggcaactat cctgctgccg gaagaaatgg
     6481 tgcggtagta caaagtgcca acaacgttct ggtggtatgg ggcggagagc agtatggcgc
     6541 ggtgggagat tttccacagt attacaatga tggcagacga tatttctggt ttgcccgcag
     6601 tatcacatca actgttccgg atattaaaac gtactatttg tataaaaaaa taatgtagta
     6661 acaccgggaa gggatttttc attttacaaa cttttgttca ataaaaccgt aaaaagttag
     6721 tttttgcttg gttttttaca cagttcatgt tgcttgcatg tgcttcgtca ctcatctttg
     6781 ttgtccttgc gatttagtaa agggctatgc ttttttatta gaaactcttc aagttcatag
     6841 cgtccaaagt gtgtcggata aaatgttgct ttgcaagact tcaaaaactc tgtaattttt
     6901 ttgtcttctg aaacttcaaa gtattttttc tttccattac gttcttgaag ttcgaagttt
     6961 aaaagttctg ttccaatgtg ccgccttagt gcagaaaagt atgttctact gctatgagtt
     7021 ttgtgtcgct cacaaatgtc tgaactctca ccgatataaa ttaatttgtc atgtttatct
     7081 ctcacaatgt aaagtcctgc gacactttca gcgttacagt caaaccaact actaaatttc
     7141 ccttgcaaaa tttcttgttc agcttgtaac aaaatatctg tttttgcttt ttgaacattg
     7201 tcccaaagtc catcctggtg agcaacattg tgcaattttc ttttgtcaag ctgaaacctg
     7261 tttaagagtg ttggtagatt tgatattcca aattcttcaa tttcttttcg cccgatgtct
     7321 gtaacgataa cttgaacttt aaatgcgtca atagaaatat tacctatcgc tttatttgtc
     7381 ttttgcaact tgagcaagtt cttgtaaaag gttgaagttg ttggtttgaa ttgttgcttt
     7441 aagcgtttgc tcaattcctt gccctcacca acgtaataat ttgtcttgtt atgagaaata
     7501 agatagttgc caaaaccatt atggtcgatg tcgctaaaca gttgatatgg tcttgttttg
     7561 agtttgtaga gtccgtccga aagaatggtg tctgaattat tttgagtaaa agtccaagtt
     7621 atttttttca taaacggaat gctcgtttag cattgcaggc aacgttttgc agctacccga
     7681 aggtggcgat ttcgaagcac ttcactgtca accaagcaga aactttgata ggagtacaaa
     7741 gcttgattta accactgaac cgccactttt gggtaggtgc tgttggctgc tgtgcttctc
     7801 ttcattgtcc ttgtctgatt tctctggtca ctatggcaga acaatcatag gagtttgtcc
     7861 ttcatagact aattcgttta ttaaaaatcg cctaaaaagt tggtcggtca aaagtcgtga
     7921 gcctttttga acaattagca attcgtcaat tttattgttt attactttct taatgtcttc
     7981 aaatgggttg ttgccttcat aaattgcaaa atctgtatta aatctatcca caaacattgc
     8041 gcataattct ctaagatgct tttctattcc ctttgttttt tcattgggtt ttgccaaata
     8101 gaaaaaggta atactcgtgt ttttgctgtc aataagattc aaaaacttgt tcaattccaa
     8161 gatgtttaaa gggtgttttt ctgttactgc cacaaatatt ttttcgtgtg aaaaagtgtc
     8221 gatttccttt ggcattgcaa caataatatt ttttgtgtta tcaacaacct gaagtgcaac
     8281 gcttccaaga aataactttt tgagtaagcc cgttcctttt attcccgtaa aaattaaatt
     8341 ttcataagga tcttctaaaa gtttaggcag tgtaagctgt aaattgcttt ccgaaacatt
     8401 gaaggaaact tttatggttg gcggaattat ttctttagct aatgccttca atttttgcaa
     8461 tgcttcttca ttagtatgtt gcgcaatttg ttgtctgctt tcattgtcgg taagtgcggg
     8521 ggctaacaca atggactgat gaaccaagag aaattctgca cttacctgtt tactccaatt
     8581 gcaagcatac tttattaagt tgcgtgaata ctccgaaaag tcaataagta tgataaatcg
     8641 ttttttcatt gcttatgttt ttgatgtgtt tggtttttgc aagctctcat taacctttgt
     8701 aaaatcttct tggctatctg caagaaccat taaaatgtct tgtgcttcta tctctgttga
     8761 accgcctgga cgaatatatt ctccgtttct tcttatcatt acgataaaag ccgattttgg
     8821 aaaattcaaa tcgacaattc ttttgttaac tgagtgaaaa tcgggtaaaa tctcaaattc
     8881 ttgtaatgat gatttaggaa gttctaaaat gagcttatct atttctgtaa tcttttttgc
     8941 attttcaggc aaagcaacat tgagccattt tgcaactatt gataatgttg ttccttgaat
     9001 cagaaccgaa gtaacagata tgaaaaacac aatattaaaa atcatatttg ctttttcaat
     9061 tcctgctaaa agtggatatg tggcaaacac aataggaaca gcaccacgta aaccaaccca
     9121 agaaatataa aatcttcttc tcagtctcat tttgaagaac atcatactga gaaatacact
     9181 tacaggtcgg gcaacaacta tcaaaaataa tgagatgagt attccaattc cgaaatatgg
     9241 tattatttga gaaggaaata caagtaaacc caaagttaag aacagcacaa tctgcattag
     9301 ccaagccaaa ccatcaaaca ttttcaaaat agttttcttg tgtataaggt cttgattacc
     9361 taaataaacc gcacaaatgt aaatagcaag aaatccgttt ccacccacaa agtcggttgc
     9421 ggaaaaagta ataaacataa gagcaataac taatgcagga taaagccctt caaagtcaag
     9481 tttgatttta ttaatgatga atttactaag tcttccgaaa gcaaaacctg cgattccacc
     9541 taaaatcatt tgttgaaaaa acaggggaat aattgaagta attccttggt cttgattgat
     9601 tactaaagtc aaaaatgcaa tagtcaatac ataagccatt gggtcattac tgccactttc
     9661 caattctaat gttgggcgta aattagtctt tagggcaaga ctttttgagc gtaaaattga
     9721 aaatacggct gctgcgtctg ttgatgaaac aatagaaccc aacaacatac tttcataaat
     9781 tgtaaagtcg gtaacatacc aaacaaatgt cccaagggaa agggctgtca gtaaaacgcc
     9841 taaagtggat aaaacaagtc cttctcttaa aattggtttt acagaagtcc aattagtgtc
     9901 aagaccgcct gaaaacaata tgaaatttag cgaaacaata ccaacgaatt gagcgatttg
     9961 aggattgtcg aaatgaatgc caccaatacc gtcagaacct gccaacattc cgattgccaa
    10021 aaagagtaat aatgtcggaa ctccaaattt gtaggatgtc ttcccgacaa caatgcttac
    10081 gaaaagcaaa agagagccaa ctaatactat attttcaatt gttaaattca ttcttttttt
    10141 tctc
//
LOCUS       DAORTI010000120        10027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7121_length_10027_cov_6.037104, whole genome shotgun sequence.
ACCESSION   DAORTI010000120 DAORTI010000000
VERSION     DAORTI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_10027_cov_6.037104"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLO70_13120"
     CDS             complement(<1..325)
                     /locus_tag="PLO70_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75454.1"
                     /translation="MKFITAIILTALLAFISGLLSFSPWWGFAVTSVLVALLVHQKAG
                     KAFLGGFLGLFLLWGGLAWWIDMKNNGVLSQKIASVLPLGGNSILLIVVTGVAGGLVA
                     GFAAMS"
     gene            complement(344..2788)
                     /locus_tag="PLO70_13125"
     CDS             complement(344..2788)
                     /locus_tag="PLO70_13125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQZ75455.1"
                     /translation="MRIVPFAVSAVVSAGLIFALNKQWGTIPPMGKFLSPQHGFWQNA
                     EPAGQSFDAELNFPELKGKAEVYFDERLVPHVFAENDEDLYFIQGYLHAKFRLFQIDL
                     QTKAAAGRASEIAGEKAVNYDKQQRRLGMVYAAENAMLEIEKDPTARKIFDAYTNGIN
                     AYIINLKESELPIEYKLLNFKPEKWSNLRSALLLKMMAKDLSSGTENDLANTNAKSIF
                     LPEELKMLYPQVPDSLVPIIPKGTVFDAPGIIPVQPATVDSLYFGKKDTVTANEIAKP
                     DINNGSNNWVVAGSKTASGAPILCNDPHLELSLPSIWYEMQLQTPGSNAYGVSLPGAP
                     FVIIGFNDSIAWGVTNAQRDVKDYYEIKFRDETKQEYWFNGKWEKTIQKPELIKVKGG
                     KDVLDTVAYTLFGPVMFDESFSNDFSNKRNLAVRWVAHDPSNEGMTFYKLNRAANYDD
                     YAEAIRTFQCPGQNFIFASKTGDIAIWQQGKFPARWNRQGLYIMPGEDSSYMWQGFIP
                     QPENPHAKNPERGFLESANQRPVDSTYPYFIPGSYITPRAITIENKLAAMSGITPKDM
                     MALHGNYFNTLAEDVRSTLVQYVNETVLNATEKKYLAIIKNWDLMASPDSKGQTVYQC
                     WWDSLEVEIWKDDLARVSPQAPWPEEQTTMELLKKDSALQFIDNINTPQKETLYDAVT
                     TALKKASADLATKEAEGKLEWTKFKNPTVYHLLKDALPAFARKGLNVGGNGNIVNAVT
                     HSHGPSWRMIVHLTNQTEAYGVYPGGQSGNPGSRFYDDYIDKWVAGEYNKLWFMREGD
                     KTDKNVKWVMKFGNSN"
     gene            2900..3460
                     /locus_tag="PLO70_13130"
     CDS             2900..3460
                     /locus_tag="PLO70_13130"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQZ75456.1"
                     /translation="MPVLIVVGIIVLIVIWVISLYNGLVRLRNRRQNAFADIDVQLRQ
                     RHDLIPQLVDTVKGYATHEKDLLLKITEARTAAMNATSIDGKIVAEQQLSAALQGLKV
                     QVEAYPDLKANQNFLQLQEELSDIENKLAASRRFFNAATTEYNNAVESFPSNLIARNF
                     GFNREILFDLGTDTRKQMDEPPKIQF"
     gene            3467..4402
                     /locus_tag="PLO70_13135"
     CDS             3467..4402
                     /locus_tag="PLO70_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQZ75457.1"
                     /translation="MQYVGLDKQIRRNNFNSTLLLIAFPALLLGMLYAILFFTTNEHG
                     EDPDELFLQSMPVVLAATGIWFLIAWSGHAAFIRMATGSKPLERMENKRVYNLVENLC
                     ISQGMQTPKIYLIEDDSLNAFASGIDKKSYAISLSRGIINKLDDEELEGVIAHELTHI
                     KNRDVRVLIISIIFVGIFAFLAELAFRSLRFAGSGRRSKDSKGSGAIILIAIVVTAVA
                     YFISILLRFGISRKREYLADAGAAEITKKPYALANALRKVSNDPAIEAVESRDVAQLF
                     FDNPKPSTHKSASWDNMFATHPSVEKRIELLEQFV"
     gene            complement(4399..5055)
                     /locus_tag="PLO70_13140"
     CDS             complement(4399..5055)
                     /locus_tag="PLO70_13140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQZ75458.1"
                     /translation="MRPTEDTYRHKGLRKKLVQGIQAKGISDERVLAAILEIPRHFFL
                     DSAFDEKAYEDIAFPIGEGQTISQPYTVAYQSQLLEIKPFQKVLEIGTGSAYQAVVLA
                     ELGAQVYTIERQKKLFEANKSFPWLKKYSSIKYFYGDGYQGLPTYAPFDRVIITAAAP
                     EVPAKLIEQLKPGGIMVIPVGAGDVQLMKRITKLDNGALKEEVFDRFSFVPMLGGRNS
                     "
     gene            complement(5123..6844)
                     /locus_tag="PLO70_13145"
     CDS             complement(5123..6844)
                     /locus_tag="PLO70_13145"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HQZ75459.1"
                     /translation="MDAAIQSKVDVWLNGNFDEATKEEIKKLQQDNPSELADAFYKNL
                     EFGTGGLRGLMGVGTNRMNKYTVGMATQGYANYLKQCFPGGVSVAIAHDSRNNSRFFA
                     ETTANVFAANGVKVYLFEALRPTPELSFAIRTLKCQGGVVCTASHNPKEYNGYKAYWD
                     DGSQLVPPHDKNVIKEVDKIASVDDVKWSGGESRITIIGKELDEQYMEMVKGLSVYPE
                     VIAKHKDLKIVYTPIHGTGITLLPQVLKNFGFDNVHIVKEQAVPDGNFPTVAYPNPEE
                     SEAMSFGLKLAEELDADILCGTDPDADRLAIGVKDSNGQWVLMNGNQTAVLAFNYLME
                     ARKTKGIAKPNDMVITTIVTTPMIDAIAKGNGVGCYRVLTGFKWIAELIRQKEGKENY
                     IIGGEESFGLMIGDKIRDKDGISAVALLCEMAAYEKEKGRSLYEKMIDLYVQYGFYKE
                     HLISITKKGMDGQQQIAAMMESYRNNPPKTINDSLVVKLLDYELQKGSNPQTGQEWKI
                     ELPKSNVLQFILADGSSISARPSGTEPKIKFYFSVNTKLNTAAEFDKVNKELDEKIKA
                     VITDMKL"
     gene            6964..8019
                     /locus_tag="PLO70_13150"
     CDS             6964..8019
                     /locus_tag="PLO70_13150"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQZ75460.1"
                     /translation="MRNFVSSVVKKTIILISLFTITGYAVAQDSTTAKKNYPSADAVI
                     AIKNVPDTVFQKGLKTGGFYKSLPPGPNKKRVWLVAGGTVVAYGGIMIGLYNAWYKNY
                     PQSGFHTFNDFPEWKQADKVGHIYSAYIESRASMEMWRWTGIDRKKRIWIGGMSGAFY
                     QTVIEVLDGFSKEWGWSWGDFGANILGSGALVAQELAWNEQRIKLKFSFHRKNYNDPE
                     LTQRSNMLFGKSAAERMLKDYNGQTYWASVNLKPFFKNSTLPEWLSVSVGYGAEGLFG
                     GTENIGKDDNSNIIFNRPDIKRYRQWYLSPDIDLTKIKTNKKGIKFIFTVLSAFKFPA
                     PSLEFSNGKFRVNALHF"
     gene            8087..8497
                     /locus_tag="PLO70_13155"
     CDS             8087..8497
                     /locus_tag="PLO70_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQZ75461.1"
                     /translation="MKKIFPLLIILTATLVMTGCGKDNNNNTPPADSPSVITITAPTA
                     GTIIINGSTLQVRGNVTDNNALSNARIEIRNTTTNAILYQQNQSTGNVLYYDLNWNWT
                     VTGITSTVNAMIRITVTDRYNYQVTKEVAVVLVD"
     gene            8577..9899
                     /locus_tag="PLO70_13160"
     CDS             8577..9899
                     /locus_tag="PLO70_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQZ75462.1"
                     /translation="MQIAQQILFVLISAVSVFLFSKKVKEISRNIKLGRDEDLSDNTS
                     RRWRNLLLLAFGQQKMFRNPLVAVMHFFVYAGFIIINIEVLEIVLDGITGKHRLFAAP
                     LGNFYSLLINAFEILALTVLVACIIFLIRRNIIKLKRFASKDLDGWPRSDANYILFTE
                     VVLMSLFLILNASDTLLQTRGVEHYAAHPTGNFIISQYIQPVLNGMSNSSLELVERGS
                     WWLHIVGIFAFLNYLPYSKHLHIVLAFPNAYYARLKPMGEMENMPSIQKEVLYAMQPE
                     LAPTGEQPAPQRFGAKDVMDLSWKNLMDAYSCTECGRCSAACPANQTGKLLSPRKIMM
                     DTRDRLEEVGKNINVNKEFKDDSRSLLHDYISVEELRACTTCNACVQECPVSISPLDI
                     IIQLRRYLVMEESNSPQEWTAMFSNVENNFAPWKFSPDERDKWVEEVQ"
BASE COUNT         2810 a         2229 c         1966 g         3022 t
ORIGIN      
        1 cactcatcgc tgcgaaaccg gctaccaatc cacctgccac acctgtaacc acaatcagta
       61 atattgaatt accgcccaat ggaagtaccg atgcaatttt ttgtgaaagg actccattat
      121 ttttcatatc gatccaccag gccaggccac cccataacaa aaacaatcca agaaacccgc
      181 ccaggaaagc ttttcccgct ttttgatgaa ccaataatgc cactaatact gaagtaacag
      241 caaaacccca ccagggtgaa aaagataata gccctgaaat aaaagccagt aatgcagtta
      301 atatgattgc agttatgaat ttcataaata tgagattata ggattagttg ctgtttccga
      361 atttcattac ccattttaca tttttatctg tcttgtcccc ttcccgcata aaccagagtt
      421 tattgtattc accagccacc catttatcaa tataatcatc ataaaaccgg ctgccgggat
      481 ttccgctttg ccctccgggg taaacgccat aagcttctgt ttgattcgta agatgtacga
      541 tcattctcca gcttggacca tggctatgcg taacggcatt tacaatatta ccgttaccac
      601 ctacatttaa ccctttcctg gcaaatgcag gcaacgcatc ttttaacaga tggtatacgg
      661 tagggttttt gaactttgtc cattccagct tcccttctgc ttcttttgtg gcaagatcgg
      721 ctgacgcttt cttcaaagca gtggtcactg catcataaag cgtttctttc tgtggcgtat
      781 taatgttatc aataaattgc agagccgagt ctttttttaa taactccata gtggtttgct
      841 cctctggcca tggtgcctgt ggtgatactc tcgccaggtc atctttccat atttccactt
      901 cgagactgtc ccaccagcat tggtagacag tttgtccttt tgaatcagga gaagccatca
      961 gatcccagtt tttaattata gccaggtatt ttttctctgt tgcattcagc actgtttcat
     1021 tcacatactg cactaatgtg cttctcacat cttcagccag tgtgttaaaa taattaccgt
     1081 gtaaggccat catatctttt ggggtaatac ccgacatagc agccaactta ttttcaattg
     1141 taatcgccct tggtgtgata taacttcccg gaataaaata aggatatgtt gaatcaaccg
     1201 ggcgctgatt ggcactttcc aaaaatcctc tttccggatt ttttgcatgc gggttttcag
     1261 gttgtggaat aaacccctgc cacatataac tgctatcctc tcccggcatt atatacaagc
     1321 cctggcggtt ccatcttgcc ggaaattttc cctgctgcca gatagctatg tcacctgttt
     1381 tggatgcaaa tatgaaattc tgtcccgggc actggaatgt cctgatcgct tctgcataat
     1441 catcataatt tgctgccctg ttcaatttat agaatgtcat cccttcattg ctcggatcat
     1501 gcgccaccca acgtactgcc aggtttcttt tattagagaa atcgttggaa aaactttcat
     1561 caaacattac aggaccgaac agggtataag ctacagtatc caatacatct ttcccaccct
     1621 ttacttttat tagttccggt ttctggattg ttttctccca tttgccatta aaccaatact
     1681 cctgttttgt ttcgtctctg aattttattt cataataatc ttttacatcc cgttgtgcat
     1741 tggtaactcc ccaggcaata ctgtcattga accctatgat aacaaaaggt gcaccgggaa
     1801 gggaaacgcc atatgcgtta ctaccaggtg tttgcaactg catttcgtac cagatagaag
     1861 gtaatgacaa ttcaaggtgc ggatcgttac aaagtattgg cgcaccactg gcggtcttac
     1921 tgccggccac tacccagtta ttactgccat tattaatatc cggctttgca atttcatttg
     1981 cagtaacggt atcctttttc ccaaagtata atgaatctac tgtagcaggt tgtacaggaa
     2041 taataccggg tgcatcaaat acagttcctt tggggataat cggcaccagg gaatcaggca
     2101 cctggggata caacattttc aactcctccg gtaaaaagat agatttcgca ttggtattag
     2161 ccaggtcatt ttctgttcct gaagaaaggt cctttgccat catttttaat aacaatgcag
     2221 aacgcaggtt gctccatttt tccggtttaa aattgagcag tttatactct atgggcaatt
     2281 ctgattcctt cagattgata atgtacgcat tgatcccatt tgtatacgca tcaaatattt
     2341 tcctggctgt cggatctttt tcaatttcaa gcattgcatt ttctgcagca taaaccatac
     2401 ccaaccgccg ttgctgtttg tcataattca cggctttttc cccggcaatt tcactggcac
     2461 ggccagcagc tgcttttgtc tgcaggtcaa tctggaataa cctgaattta gcatgcaggt
     2521 aaccttgtat aaaataaagg tcttcatcat tctcggcgaa tacatgcggc actaatcgct
     2581 catcaaaata aacctctgcc tttcctttga gctcaggaaa atttaactcc gcatcaaaac
     2641 tttggccggc gggttctgca ttctgccaga aaccatgttg aggacttaag aactttccca
     2701 tcggcgggat ggtaccccat tgtttgttaa gggcaaaaat taatccggcg gaaacaactg
     2761 ctgaaacagc aaaaggtaca atacgcatag gcaagaaaat gaattcgaaa gataagaaga
     2821 aatgtaacag gaattttatt ccaaatcatg atctgtcagt ttctttttgt gtaatttcaa
     2881 tatctctaaa aacacaacta tgccagtatt aattgtagtc ggtattatcg tcctgatcgt
     2941 tatttgggtt atcagcttgt ataacggact ggttcgtctc cgcaaccgtc gccaaaatgc
     3001 ttttgccgat attgacgtgc agcttcgcca gcggcatgac cttatcccgc aattggtaga
     3061 taccgtaaaa ggatatgcca cgcatgaaaa agatctgttg cttaaaatta cagaagcccg
     3121 gactgctgca atgaacgcca ccagtattga cgggaagatc gttgcagaac aacaactctc
     3181 cgcagctttg cagggtttaa aagtgcaggt agaagcatat cctgatctga aagccaatca
     3241 aaatttttta caactgcagg aagaacttag tgatattgaa aataagctgg ctgccagccg
     3301 ccggttcttt aatgcagcca ccacagagta taacaatgcg gtggaatctt tcccgagcaa
     3361 cctgatcgca agaaattttg gtttcaaccg ggagatcctg ttcgacctgg gtacagatac
     3421 ccgcaaacaa atggatgagc ctccgaaaat tcaattttaa ttgctgatgc aatacgttgg
     3481 actagataag cagattcgtc gaaataattt caattcaacc ctcttgttaa tagcattccc
     3541 ggctttattg ctggggatgc tttatgctat tcttttcttt accacgaacg aacatggcga
     3601 agaccctgat gagctattcc tgcaatccat gcctgtagtt ttagcagcta ccggcatctg
     3661 gttcctcatt gcatggtctg gccatgcagc ttttatccga atggccaccg gcagcaaacc
     3721 gctggaaaga atggaaaata aaagggtcta caacctggta gagaatcttt gtatatcaca
     3781 aggcatgcaa acgcctaaaa tctacctgat tgaagatgat tccctaaatg cttttgccag
     3841 tggtattgat aaaaaaagct atgctatctc tttatccagg ggtattatta ataaactgga
     3901 tgatgaggaa ctagagggtg ttattgcaca tgagctaacc catatcaaaa accgggatgt
     3961 tagggttttg atcatctcta tcattttcgt tggcatattt gctttcctgg ctgaattggc
     4021 atttcgcagc ctgcggtttg ccggatcggg aagaagaagt aaagacagta aagggagtgg
     4081 tgctatcatt cttattgcca ttgttgttac ggccgttgcc tatttcatct ccatcttatt
     4141 acgtttcggt atcagccgga aaagagaata tttggctgat gcaggtgcgg cagaaattac
     4201 caagaaaccc tatgcgttgg ctaatgcctt gcgtaaagtt tcaaatgatc ctgccataga
     4261 agcggtggaa agccgtgatg tagcacaatt attttttgat aatcccaaac cttctaccca
     4321 caagtctgca tcctgggata atatgtttgc cacgcatcca tcggttgaaa aacggattga
     4381 gttactggaa cagtttgtct aagaattcct gcctcccagc attggaacaa aagaaaaacg
     4441 atcaaatact tcttctttca atgctccatt atccagttta gtgatgcgtt tcattagctg
     4501 tacatcacca gctcctacag gtatcaccat aataccgcct ggctttaact gctcaataag
     4561 tttggccggc acttccggag cggcagctgt aataattacc ctgtcaaagg gtgcataagt
     4621 aggtaatccc tggtagccat ccccataaaa atattttatg gaagaatatt tttttagcca
     4681 tggaaacgat ttgtttgctt caaacaattt cttttgtcgt tctattgtgt atacctgtgc
     4741 gcccaattcc gccagcacaa ccgcctggta agcactgccg gtaccaattt ccaatacctt
     4801 ctgaaagggt tttatttcga gtaactggct ttgataagct actgtataag gttgtgagat
     4861 tgtttgtcct tcaccaatag gaaaagcaat atcctcgtat gctttttcat caaatgctga
     4921 atccaaaaaa aaatgacggg gtatttctag tattgcagcc agcacccgtt catcagaaat
     4981 tccttttgcc tgtatgcctt gtactaattt cttgcgaagg cctttgtgcc ggtatgtatc
     5041 ttctgttggt ctcataagcg tagcaaaaat aaaaaaagca gcatcgtaaa aatgccgctt
     5101 tgatgaacta ttcaaaaagt gtttacaact tcatatccgt gatcaccgcc tttatctttt
     5161 catccaattc cttattcact ttatcaaact cagctgccgt attcagtttt gtattcacac
     5221 tgaaatagaa cttgatctta ggttcggtgc cgcttggtct tgcagaaata ctgcttccat
     5281 ccgctaatat gaattgcaac acattcgatt tcggtaactc tattttccac tcctgccccg
     5341 tttgcggatt gcttcctttt tgcaattcat aatccagcag ttttaccacc agtgagtcat
     5401 taatggtttt tggcggatta ttgcggtagc tttccatcat cgctgcaatc tgctgctggc
     5461 catccatacc tttctttgta atggagatca ggtgttcctt ataaaaacca tactgcacat
     5521 acaggtcgat cattttttca tagaggctgc gtcccttttc tttttcatac gctgccattt
     5581 cgcataacaa tgctacggca ctgatgccat ccttatctct aatcttatca ccgatcatta
     5641 atccaaaact ttcttcaccc ccgatgatat aattttcttt cccttctttc tgccggatta
     5701 attcagcgat ccatttaaag ccggtcagaa cccggtagca acccactcca tttccttttg
     5761 ctatcgcatc gatcataggt gtggttacaa tggtagtgat caccatatca ttgggctttg
     5821 caatgccttt tgtctttctt gcttccatca ggtagttaaa agccagtact gcagtctggt
     5881 taccattcat cagcacccat tggccattgg aatcttttac accgatcgca agacggtctg
     5941 catccgggtc ggtaccgcaa agaatatctg catccagctc ttctgccagt ttcaatccaa
     6001 aactcatcgc ttcactttct tccgggttag gataagccac tgtggggaaa tttccatccg
     6061 gtacagcctg ctcttttaca atatggacat tatcaaaccc gaaatttttc aatacctgcg
     6121 gcaataaagt aataccagtt ccatgtatag gagtataaac gatcttcagg tctttatgct
     6181 ttgcaataac ttcgggatac acacttaatc cctttaccat ttccatatat tgctcatcca
     6241 gttctttgcc gatgatggtt atcctgcttt cacccccgct ccatttcaca tcatccactg
     6301 aagcaatttt atccacttct ttgatcacat ttttatcatg cggcggtact aattgagatc
     6361 catcatccca ataagcttta tacccattat attctttcgg attatgcgaa gcagtacaaa
     6421 ccacaccacc ctggcacttt aaggtgcgga tagcaaagga taattctggt gtagggcgaa
     6481 gtgcttcaaa taaataaacc ttcacaccat tggccgcaaa tacattagcc gttgtttctg
     6541 caaagaaacg gctgttgtta cgactgtcat gtgcaatagc tacactgacg ccgccaggaa
     6601 aacattgttt caggtaattg gcatagccct gggtggccat accaacagta tatttattca
     6661 tccggttggt acccacaccc atcagtccgc ggagaccacc ggtaccaaat tccaggtttt
     6721 tataaaatgc atctgccagt tcggatggat tatcctgttg taattttttt atttcctcct
     6781 ttgtagcctc atcaaaattt ccattcagcc atacgtctac tttagattga attgcagcgt
     6841 ccataatcga tatttctggt ttttgataat aaagctgaaa gttattgaat tttatgtttt
     6901 gaaactgaaa agaaaccaga ttcgtaaatt tgatttttta taataatatc ccctcaatac
     6961 tctgtgcgaa actttgtgtc ttctgtggta aaaaaaacaa tcatactcat ttccttattt
     7021 actattaccg gctacgctgt tgcccaggat tctactactg ccaaaaagaa ctacccgtca
     7081 gctgatgcag ttattgctat aaaaaatgtc ccggatactg tattccaaaa aggtttgaaa
     7141 accggcggtt tttataaatc gcttcccccc ggaccaaata agaaacgggt ctggctggtt
     7201 gcaggcggca ctgttgttgc ctatggcggc attatgattg gcctttacaa tgcctggtat
     7261 aaaaattatc cgcaaagtgg ttttcatacg ttcaatgact tcccggaatg gaagcaggca
     7321 gataaggtag ggcatatcta cagtgcctat attgagagcc gggccagcat ggaaatgtgg
     7381 cgatggacag gtatagacag gaaaaaaaga atctggatcg gcgggatgag cggcgctttt
     7441 tatcaaactg tcattgaagt gctggatggt ttcagtaaag aatggggctg gagttggggc
     7501 gactttggag caaatatatt aggcagtggc gccctggttg ctcaggagct ggcctggaat
     7561 gaacagcgta taaaacttaa attttctttt caccggaaaa actataacga cccggaatta
     7621 acgcaacgca gcaatatgct ttttggaaaa agtgctgcgg aacgcatgtt aaaagattat
     7681 aatggtcaaa cctattgggc cagtgtaaac ctgaaaccat tttttaaaaa ttcaaccctg
     7741 cctgaatggc tttccgtttc tgtgggctat ggtgccgaag gtttatttgg gggcactgaa
     7801 aatattggca aggatgataa tagcaatatc attttcaaca ggccagatat aaaaagatac
     7861 cggcaatggt acctctctcc tgatattgac cttactaaga tcaaaaccaa taaaaaggga
     7921 ataaaattta tattcaccgt gctgagtgct tttaaattcc cagcgccttc actggagttc
     7981 tccaatggca agttcagggt aaatgcactg cacttctgac caattacatg aattaatgta
     8041 aattagctct tccatccttt taatacataa atgaattctg acatatatga aaaaaatatt
     8101 ccccctgcta atcattctga cagcaacttt agtgatgact ggctgcggca aagacaacaa
     8161 taacaatacc ccacctgctg atagtccctc tgtcatcaca atcacagccc ctactgccgg
     8221 aacgataatt attaatggaa gcaccctaca ggttagaggt aatgtgacag ataacaatgc
     8281 cctttccaat gccaggatcg aaataaggaa taccaccacc aacgctattc tataccagca
     8341 gaaccaatct accggcaatg ttctttacta cgacctgaac tggaactgga ccgtaaccgg
     8401 tatcacctct actgtaaatg caatgatcag gatcactgtt acagacaggt ataattacca
     8461 ggttacaaaa gaagtggcag tggtactggt tgattaaata tgtaaaccgg cattttttca
     8521 acaattcgta ttattcattg ctgcatcccg taaattagca gctgtaatta actgctatgc
     8581 aaattgccca acaaatctta tttgtcctta tcagtgcggt ttctgtcttt ttgttttcaa
     8641 aaaaagtaaa agagatcagc cgtaatatta aactgggaag agacgaagac ctgagcgaca
     8701 atacttcccg tcgctggaga aatcttttat tactggcttt cgggcaacaa aaaatgtttc
     8761 gcaacccgct ggtggcagtg atgcactttt ttgtatatgc tggttttatt attattaata
     8821 tagaagtgct ggagatagta ctggatggca tcaccggtaa gcaccggtta tttgccgccc
     8881 cgctgggtaa tttttattcc ctgctcatca atgcgtttga aatactggca ttaactgtac
     8941 tggtggcttg tattattttt ttgataagaa ggaatattat caaactaaaa cgttttgcca
     9001 gtaaggacct tgacggctgg ccaagaagtg atgcgaatta catactgttt accgaagtcg
     9061 tgctaatgtc gttgtttctt attctgaatg ccagcgatac attattgcaa acaaggggtg
     9121 ttgaacatta tgctgcacat ccaacaggaa attttataat ttctcaatat atacagcctg
     9181 tattaaacgg aatgagtaac agcagccttg aactggtgga gagaggttcg tggtggctgc
     9241 acatcgttgg gatctttgca tttttgaatt acctgcctta ttccaaacac cttcatattg
     9301 tattggcttt ccccaatgcc tattatgcaa gactgaaacc aatgggtgaa atggaaaata
     9361 tgccatccat tcaaaaagag gtgctgtatg ctatgcagcc ggaactggct ccaaccggtg
     9421 aacaaccagc accacaaaga ttcggggcaa aagacgtaat ggacctgagc tggaagaacc
     9481 tgatggatgc ttatagttgt acagagtgtg gccgctgctc tgctgcatgt cctgctaacc
     9541 agacaggcaa actgctttct cccaggaaaa taatgatgga tacccgtgac aggctggaag
     9601 aagtgggaaa gaatataaat gtcaacaaag agtttaagga cgatagcagg agtttgcttc
     9661 atgattatat ctctgttgaa gaactccgtg cctgtaccac ctgcaatgcc tgtgtgcagg
     9721 aatgcccggt cagtatcagt ccgctcgata tcattataca actccgccgt tacctggtca
     9781 tggaagaaag taattcgccc caggaatgga cagccatgtt cagtaatgtg gagaataatt
     9841 ttgccccgtg gaagtttagt ccggatgaga gagataaatg ggtggaggaa gtacaataaa
     9901 ttaaaaaata attatgcctt atagaattat attttttgta attactaact tgcttttata
     9961 ctcttgttca agagaaggca atgatgattt ttatattaac gcaacttttc aaactgaaag
    10021 cattgca
//
LOCUS       DAORTI010000121        10014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7128_length_10014_cov_5.917060, whole genome shotgun sequence.
ACCESSION   DAORTI010000121 DAORTI010000000
VERSION     DAORTI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_10014_cov_5.917060"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2286
                     /locus_tag="PLO70_13165"
     CDS             166..2286
                     /locus_tag="PLO70_13165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ75463.1"
                     /translation="MAIIIKIHTLLLALFFFCQSLIGQNIQAIVNSPVTDKQKADTLL
                     SIGKKYHMRFKVDSALLVFEKGLSYAKKTGDPTLVAIYYIVTSRTYRLQGKSQTALET
                     IRKATPYISTKTPEGTLENYFLNTGHYFKDLSISDSALYYYGETEKLNNKYFPYENWV
                     VYESMAGIFAENLAWDKAEEYYLKGYAITKAGNKRADHGVILVMLGNLYVMANKPDKF
                     TTLLNEYELFMSASRKNLLQDPVHNMLFLDWSKYSLEQKVVFLEKAKKKNLENGYQEG
                     AEACNLYLSMAFEEAKLYDKALEYLKENYQSNRSGIDLYYKHINLRHIYRIQKKAAMD
                     VAALKTAEELLSLITKLSDASSKEITLNLEKKYETEKKEKEIALLNSNNELARKEIAL
                     LNSQKKLIDIELLRQLDIQKALARENELMDSVVKGEKAYSISVTREKEKEAALNAALG
                     RENILKAGELDKEKSLRQVLIGGASLLLVAVGIILFQFRKQRLKSYVIQKQSDDLQVL
                     MKEIHHRVKNNLQVISSLLDLQSMTIADNQASEAVKEGKNRVQSMALIHQNLYSEGNI
                     KGIKTKDYISNLLQSLCDSYNITNDKVKIKTDIDDLNLDVDTMIPLGLVLNELVSNSM
                     KYAFKDGRQGELSIILKEKAQHLLLKVSDNGTGYPDGLNVKESKSFGMKMIRAFAQKL
                     KAKLDVYNHNGAVVEMQITKYNLA"
     gene            2283..2906
                     /locus_tag="PLO70_13170"
     CDS             2283..2906
                     /locus_tag="PLO70_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75464.1"
                     /translation="MSKFRVLIVEDEPVIAENISMYLDNNDFEVSGIAYDSGEAIEQL
                     QNNTPDAAILDINLESEEDGIDIANFINQRYQIPFLFLTSYSDKETLQRAKNVNPSGY
                     IVKPFNEKTLLASLEIAINNHAADTNKKMPLLTMDKINRYLLSPLSDREFEVVQLIYE
                     GITNNQIAERIFVSVNTVKTHLKNIYLKLDANTRYGVIIRLRELISK"
     gene            2988..3452
                     /locus_tag="PLO70_13175"
     CDS             2988..3452
                     /locus_tag="PLO70_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75465.1"
                     /translation="MKKIFFLSVLFYGLLFGSARAQQNRKPANKKTATSDGITTTKPA
                     KQPGNTGWIDDSLLWVKNPENGKVGTTAKGNAAKTTATNSRTTQPVKKPGNQGWIDDS
                     LLWIKNPANGPYIRNQRTAPGKNQPVNNTKHDDLKNPFDTTKKAKPAKAVRN"
     gene            3659..4039
                     /locus_tag="PLO70_13180"
     CDS             3659..4039
                     /locus_tag="PLO70_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75466.1"
                     /translation="MVQVDISQLSRECNAWREQLRNFRNELTEDETKIRQVAGQSLSR
                     EQLLEVEHLHNQFHIQLINIHDLKQSIKVHDRKMNFEQAVFNGTANEDSLSRHENLFA
                     EFQSLEQKLEELRTEFNEFIKRTP"
     gene            4165..6300
                     /locus_tag="PLO70_13185"
     CDS             4165..6300
                     /locus_tag="PLO70_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQZ75467.1"
                     /translation="MPEFDTSHVKNIVLLGHAGSGKTTLAECMLYEAGIINRRGSIAE
                     RNTTGDYHELEQERGNSIFSKLLHTKWKGYKINIIDTPGYDDFAGEVISALKVADTGV
                     MLLNAVMGVEVGTDIIWDYTERFKTPMILAVNKLDDDNADFDKTVREAKAHFGSNVVV
                     VQYPRQTGAGFHEVIDVLTMTMYKFNGNGGKPEKLPIPDEEKAKADQLHKELIEAIAS
                     NDETLMEKYFDKGELNEDEMKIGLKKAMINHDLFPLFSLSAERNMGSGRLMGFIDNVC
                     PSANEMPPQKTRKGDLLTCDANGPACIFIYKTISEPHVGELSFFKVYSGVVKSGMELE
                     NENTGVTEKISQLFLVEGNKRTSINELVAGDIGATLKLRNTHVNNTLHIKGKNYDLPS
                     IEFPPPNMTVAIETLKKGEEEKLSQALHQLREEDPTLLVEVSSELKQTLIHCQGDMHL
                     AVAKWKIEHQHKLEVKFVKPKIAYRETIRKMADANYRHKKQSGGAGQFGEVYLRIEPW
                     YEGMPEPTDLNVRGRDVHDLEWGGKLVFYNCIVGGAIDARFLPSVLKGVMEKMQNGPL
                     TGSYARDIRVCVYDGKMHPVDSNDISFKIAGLQAFRQAFQQADPQILEPIYQLDVLCP
                     DDLTGAVMGDLQSRRAIVEGIDNEGHFQKIIAKVPLAEMDGYSSSLRSITQGRAKFKM
                     KFNGYEAVPFELQRKLIDDYSKTTKEEFA"
     gene            complement(6368..7558)
                     /locus_tag="PLO70_13190"
     CDS             complement(6368..7558)
                     /locus_tag="PLO70_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75468.1"
                     /translation="MYKKVFSVIFFLAIVLTAISQNYVTLHEDCNFKGKSYYLEAGNH
                     RLYQIKIGNDRLSSIQIPFGFKVTIYADDNFSGISKTFTENISCLEPEWNNMASSIVV
                     ENTNYLQGNLSDYVVFYNDCYSKGFSRSLRPGLYKGTDLGELKNNISSFNIFGNLRVR
                     VFINNENASGYYNNFDSDQSCLGNSYNDKISSLVIEYKPDLPGNTTGLSNGKFATFYA
                     ECSYSGNALRLMPGYYSGEKLGLLKYSIASVEIPAHLWVKVFINNDNLNGQSATLSEN
                     SSCLDYNLRNKIGSLVVEEKEGGNYGGGNNLPVTETVMIYSDANYRGQSVSVLPGTYT
                     TMALAGGFPDNALSSIKVPSGFRVVLYESENFRGKSFTISESRSGFTAAWNDKISSFV
                     VYRN"
     gene            complement(7611..8285)
                     /locus_tag="PLO70_13195"
     CDS             complement(7611..8285)
                     /locus_tag="PLO70_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQZ75469.1"
                     /translation="MDRRKSLKLIATGAVAVPAVIAGCNSDDKKADAIKAEEPKFNLD
                     RNPDELKYEKEILAKERFFTDHEMATITLLSDIIIPKDEISGSASEAKVPEFIEFIVK
                     DMPSHQVPMKGGLRWLDLQCIKRFEKSFKDCSQQQQIELVDMIAFPDKAKPEMSQGVQ
                     FFRLMRDLTATGFYTSEMGIKDLDYKGNQPNQWNGVPAEVLAQYKLAYTEKELKECVS
                     FNNNSL"
     gene            complement(8344..>10014)
                     /locus_tag="PLO70_13200"
     CDS             complement(8344..>10014)
                     /locus_tag="PLO70_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQZ75470.1"
                     /translation="AAYMLAKAGLKVCIIKAGYMYDPQKNITQLKNPWDSPRRGASTK
                     LRPFGDFDACYGGWSVEGEPFTKEAGTDWMWWRARMLGGRTNHWGRISLRFGPKDFKR
                     KSIDGLGDDWPIGYDDIKPYYDRVDKLIGIFGTNEGLENDPDGFFLPPPKPRLHELFI
                     KNAAVQAGVKVIPSRLSILTKPIENDAGRGACFFCGQCNRNCSMAKADFSSPNVLIYP
                     AMKTGNIDVVANAMAREVLTNSEGLATGVSYISKVDMMEYQVEGRVVIVAASACESAR
                     LLLNSKSQRHPNGLANSSNVVGKYLHDSTGAALGGVLPQLFDRKRYNEDGVGGMHIYS
                     PWWLDNKKLDFPRGYHIEYWGGMSQPSYGFSWGIENLNGKYLVKGKQKEAGGYGASLK
                     EDYRFFYGAGVGMAGRGEAIPLESNYCAIDPDVVDKFGIPVLKFNVKWSEHEVKQAKH
                     MKETFKEVMHNMGAIITWGGDDDEKNQWGLAKPGEIIHEAGTVRMGNDAKKSALNKWS
                     QAHDCKNLFCVDGGQFVSQADKNITWTILALSMRASEYIIEEMKKQNL"
BASE COUNT         3206 a         1995 c         1964 g         2849 t
ORIGIN      
        1 tttcgcaaaa caatatagca tcaaataaaa aaaactaatt aatcaataaa aatctctgtt
       61 tctaaaggat tatttcagag ataatgaaac ccatgtagca aaaaagattt aaatctcttc
      121 tgattgaaat cataaaaaag tttattttag agatagctgt aaaaaatggc cattatcatt
      181 aaaatacata cactcctgtt ggcattattc tttttctgtc agtctttaat agggcagaat
      241 atacaggcta ttgtgaatag cccggttaca gacaaacaga aagctgatac ccttctttca
      301 atcgggaaga aatatcatat gcggtttaaa gttgacagtg ctctccttgt ttttgaaaaa
      361 ggactttcgt atgcaaaaaa aacaggtgat cccacccttg ttgctattta ttatattgtt
      421 accagcagaa cttaccggtt gcagggaaaa tcacaaacag cacttgaaac catacgcaaa
      481 gcaactcctt atatcagtac aaaaacaccg gaaggcactc tcgaaaatta ttttctgaat
      541 accgggcatt attttaagga tctgtcgatc tcagattctg cgctgtatta ttatggtgaa
      601 acagaaaaat tgaacaataa atatttccca tatgagaatt gggttgtata tgagagtatg
      661 gcgggaatat ttgctgaaaa tttagcctgg gataaggcag aggagtatta tttaaagggt
      721 tatgcgatta ccaaagccgg gaataaaaga gctgaccatg gagtgatact ggtgatgctg
      781 gggaatttat acgtaatggc caacaagccg gataagttca ctactctttt gaatgagtat
      841 gagctattca tgagtgcatc ccggaaaaat cttttgcaag atcctgtgca taatatgtta
      901 ttcctggact ggagcaagta cagtttagaa caaaaagtag tattcctgga gaaagcgaaa
      961 aagaaaaacc tggaaaatgg ctaccaggaa ggcgctgaag cctgtaattt atatttatcc
     1021 atggcttttg aagaggcaaa gttgtatgat aaggcattgg agtatttaaa agaaaattat
     1081 caatcaaaca gatcagggat agatttatat tacaaacata taaacctgcg gcatatttat
     1141 cgtattcaga aaaaagctgc aatggatgtt gctgcattaa aaacagcaga ggaactttta
     1201 tcactcatca ctaaactttc agatgcttcc tcaaaagaaa taacgcttaa cctggaaaag
     1261 aagtatgaga ctgaaaaaaa agaaaaagaa atagccttac tgaattcaaa taatgaattg
     1321 gcaagaaagg aaatcgcttt attaaattct caaaagaagc tcattgatat cgaattactc
     1381 cggcagctgg atattcaaaa agcactggca agggaaaatg aattgatgga ctcagttgtt
     1441 aagggtgaaa aagcctatag tatatcagta acaagagaaa aagaaaagga ggcagctctc
     1501 aatgcagctt taggacgtga aaatatactc aaagccggtg aacttgataa agaaaaaagt
     1561 ctgcgccagg tgcttattgg cggagcttca ttactattag tggctgttgg aatcatttta
     1621 tttcaattca ggaagcaacg gttgaaaagc tatgtgatac aaaagcaatc tgatgatctg
     1681 caggtgctga tgaaagaaat ccaccaccgt gttaaaaaca acctgcaggt tataagcagc
     1741 ctgctcgatc tgcaaagtat gactatcgcc gataaccagg caagtgaagc tgtaaaagaa
     1801 gggaaaaacc gggtacaaag tatggcctta attcaccaga acctttacag tgaaggcaat
     1861 attaaaggca taaaaacaaa agattacata agcaaccttc tccagagtct ttgtgattct
     1921 tataatatta caaatgataa agtgaaaatt aaaacagata tagatgacct gaacctggat
     1981 gttgatacaa tgatcccgtt ggggctggtg ctgaatgagc tggtgagtaa ctcaatgaaa
     2041 tatgcattta aagatggaag acaaggtgag ttgagcatta tcctcaaaga aaaagcacaa
     2101 cacttactgc tcaaagtcag cgataatggt acaggctatc cggatgggct gaatgtgaag
     2161 gagagtaaat catttggcat gaagatgata agggcttttg ctcagaaatt aaaagcaaaa
     2221 ctggatgttt acaaccacaa cggagctgta gtggaaatgc aaataaccaa atacaacctg
     2281 gcatgagtaa attcagggtg ttgatagtag aagatgaacc cgtcattgca gagaatatct
     2341 ctatgtatct tgacaataat gattttgagg tcagcggtat tgcctatgat agcggggaag
     2401 ctattgaaca attgcaaaat aacacccctg atgctgctat acttgatata aatcttgaaa
     2461 gtgaggaaga cggcattgac attgccaact tcatcaatca aaggtaccag ataccttttt
     2521 tattcctcac ttcctattcg gataaagaaa cactgcaaag ggccaagaat gtaaatccca
     2581 gcggctacat agtgaaacct tttaatgaaa aaactttact ggcttcactt gaaattgcca
     2641 tcaataatca tgcagctgat actaataaaa aaatgccgtt gctgacaatg gacaagatca
     2701 acagatacct attgtctccc cttagcgacc gggaatttga agtggtacag ttgatttatg
     2761 aaggcattac caacaaccag atagcagagc gaatatttgt ctctgtgaac accgtaaaga
     2821 cccacctgaa aaatatttac cttaaactgg atgccaatac acggtacgga gttattatca
     2881 ggctccggga actgatcagc aaataaattt aacttttaaa aatcacccgg atgggtgagt
     2941 ggttgatagt tggtttataa caattttatc tttctaaacc aaagatcatg aaaaaaatat
     3001 tctttctttc cgttctattt tacgggttgc tgtttgggtc tgcccgggct cagcaaaaca
     3061 ggaagccggc taacaaaaaa actgcaacat ctgatggcat cactacgaca aaaccagcaa
     3121 agcagccagg aaatacaggc tggatagatg attctttact ctgggtaaaa aatcccgaaa
     3181 atgggaaagt tggcactaca gctaaaggga acgctgcaaa gacaacagcc accaatagca
     3241 gaacaaccca accggtaaag aaaccaggaa atcagggttg gatagatgat tccttactct
     3301 ggattaaaaa tcccgcaaac ggaccttata tcagaaatca acgaactgcc ccaggtaaga
     3361 accagccagt taacaataca aaacatgatg atctgaaaaa tccatttgat accacaaaaa
     3421 aagcgaaacc ggcaaaagcg gtacggaatt agctttatat tttccttgca accactataa
     3481 acgaggctgt ccttttgggg gcagcctcgt ttcttatttg aattttttta tcaaatattt
     3541 gattttacct aaaatcgtac taacttacga ataacacaaa cccgcctctt cttaaatcca
     3601 acaccccagg gaaaattaaa aagttctttt aatttttaac ttttaaattt taaatcccat
     3661 ggttcaggta gacatctcac agctctccag ggagtgtaat gcatggcgtg agcaattacg
     3721 taattttcgt aatgaactca cagaagatga aacaaaaatc cgtcaggtag caggccaatc
     3781 actctccagg gaacaacttc tggaagtaga gcatttgcac aaccagtttc acatccagct
     3841 tatcaacatt catgatctga aacagtctat caaagtacat gaccgtaaaa tgaactttga
     3901 acaagctgtc tttaatggca cagctaatga agattcatta agccggcatg aaaatttgtt
     3961 tgctgaattc cagtcgctgg aacaaaaact tgaggaactc cgtacagaat tcaacgaatt
     4021 tattaagcga acgccttaaa ttaatcataa tattctgctg attttaatca tcttaggacc
     4081 taaccgggtt catatgatta gttgctgtcc ttttgaagat ttgtaactgc aaaaaacaat
     4141 ttcttcaaca ataaatttat tcccatgcca gaatttgata cctcacatgt aaaaaatatt
     4201 gtcttgctgg gccatgccgg ctccgggaag accaccttag cggaatgtat gctatatgaa
     4261 gctggcatta ttaaccgcag aggttctata gcagaacgga acaccactgg cgattaccac
     4321 gaattggaac aggaacgtgg taattctatt tttagcaaac tcctgcacac gaaatggaaa
     4381 ggctataaga tcaatattat tgataccccg ggctatgatg actttgccgg cgaagtgatt
     4441 tctgcattaa aagtagcaga tacgggtgta atgcttttaa atgcagtgat gggggtggaa
     4501 gttggcacag atatcatctg ggattataca gagagattta aaacgccaat gattctcgct
     4561 gtaaataaac tggatgacga caatgcagac ttcgataaaa ctgttcggga agccaaagca
     4621 cattttggta gtaatgtagt ggtggtgcaa tatccccgcc agaccggtgc cggctttcat
     4681 gaggtgattg atgtgcttac aatgacgatg tacaaattca atggtaacgg gggtaaacct
     4741 gaaaaattac ccattcctga tgaggaaaaa gcaaaagctg atcagttgca taaagagctt
     4801 attgaagcaa ttgccagtaa tgatgaaaca ctgatggaga agtatttcga caaaggtgaa
     4861 ctgaatgaag atgaaatgaa gatcgggtta aagaaagcaa tgatcaatca cgacttgttc
     4921 cccttatttt ccctttctgc tgaacgtaat atgggtagcg gaagattaat gggttttatc
     4981 gataatgttt gccccagtgc taatgaaatg ccacctcaaa aaacgagaaa aggagatctt
     5041 ttaacctgtg atgctaacgg gccggcctgc atctttattt acaaaaccat ttctgaacct
     5101 catgtggggg aattatcatt ctttaaagtt tattccggtg ttgttaaaag cggaatggag
     5161 ctggaaaatg aaaatactgg tgttacagaa aaaataagtc agctcttttt agtagaaggt
     5221 aataaaagaa cttcgatcaa cgaactggta gcgggtgata taggcgcaac ccttaaactc
     5281 aggaacacac atgtcaataa taccttgcat ataaaaggta aaaattatga tttgccgtcc
     5341 attgaatttc ctccgcctaa tatgactgtt gccattgaaa ccttgaagaa aggggaggag
     5401 gaaaagcttt cccaggcatt acaccaatta agagaggaag atcctacatt attggtggaa
     5461 gtttcttctg aattgaaaca aacgctaatc cattgccagg gtgatatgca cctggcagtt
     5521 gccaaatgga aaatagaaca ccagcataaa cttgaagtaa aatttgtaaa acctaaaatt
     5581 gcataccggg aaactatccg taaaatggcc gatgccaatt atcgtcacaa aaagcaaagt
     5641 ggtggtgcag gccagtttgg tgaagtatac ctgcggattg aaccctggta tgagggaatg
     5701 cctgaaccta ctgaccttaa tgtgaggggc agggatgtgc atgaccttga atggggtggt
     5761 aaactggtat tttataactg cattgttggt ggagcaatag atgccaggtt tctcccttcc
     5821 gttttgaaag gggtaatgga gaaaatgcag aatggccctc ttacaggttc ttatgcaagg
     5881 gatatccggg tatgtgtgta tgatggaaag atgcacccgg tggacagcaa tgatatttcc
     5941 tttaaaattg ccggcctgca ggcatttcgc caggctttcc agcaagccga ccctcaaatt
     6001 ctggagccca tttatcaact ggatgtactc tgtccggatg atctgaccgg agctgtgatg
     6061 ggcgatctcc aaagccgcag ggctatcgta gagggaattg acaatgaagg acattttcag
     6121 aaaataatag ccaaagttcc gctggctgaa atggatggtt attcatcttc gcttcgttcg
     6181 attacacagg gaagggccaa attcaagatg aaatttaatg gatatgaagc tgtcccattt
     6241 gaattacaga ggaagttgat agatgattat agcaaaacta ccaaggagga atttgcataa
     6301 tacttttcaa aaagtaaaaa agggctgcac aattttgaac agcccttttt ttaattccag
     6361 cctcacctca attcctgtaa actacaaagg atgatatttt atcgttccag gcagctgtaa
     6421 aaccactgcg tgattcgctg atggtaaaac tttttcccct gaagttttct gactcataaa
     6481 gtacaacccg gaatccagat ggtactttta tagaacttag tgcattatcc gggaaaccac
     6541 ctgccagggc catagttgta taagttccgg gtaatacaga aactgattgc ccccggtaat
     6601 tagcatctga ataaatcatg actgtttctg taacagggag gttgttgcca cctccataat
     6661 tcccgccttc cttttcttca acaaccagtg atccgatctt attcctgaga ttataatcaa
     6721 ggcagctact attttcagaa agtgtggcag attgcccatt taaattgtca ttattgatga
     6781 aaacttttac ccacaaatga gcaggaattt ctacagaggc tatgctatac ttcaaaagac
     6841 ccagcttttc tcctgaataa taacccggca ttaatcgtaa agcattaccg gaataactgc
     6901 attcggcata aaatgtggca aatttcccat ttgataggcc agtagtgttt cccggtaaat
     6961 caggtttgta ttcgattacc agcgaactga tcttatcatt ataactgttt ccaaggcaac
     7021 tttgatcgga atcgaaattg ttataatacc ctgaagcatt ctcattattg atgaatactc
     7081 ttacccttag attgccaaaa atattaaatg atgaaatgtt attctttagt tcgcccaggt
     7141 cagttccctt atagagtcca ggccgcaggc tgcgggaaaa tccttttgaa tagcaatcat
     7201 tataaaaaac gacatagtca gaaagatttc cctgcaggta gtttgtattt tccactacaa
     7261 tagaagatgc catattattc cattcaggct caagacagga tatattttca gtaaatgttt
     7321 tggaaatccc gctgaaatta tcatcagcat agatagttac tttaaatcca aatggtattt
     7381 gaattgaaga taacctgtca ttaccaattt taatctgata taaacggtga ttacctgctt
     7441 ccaggtaata gcttttcccc ttgaaattac aatcttcatg aagggtaaca tagttctgcg
     7501 aaattgctgt taacacaatg gccaggaaaa atataactga aaagactttc ttatacatat
     7561 gtatataatt ttattgttga gtaataagaa agtgtaaata ccttaactgt ttataaagaa
     7621 ttgttattga aacttacaca ttccttcaat tccttttcag tatatgccag tttgtattgt
     7681 gccaatactt ctgcgggaac gccattccac tggtttggct gattgccttt ataatccagg
     7741 tctttaatac ccatttccga ggtgtagaac ccggttgctg tcaggtctct catcagccgg
     7801 aaaaattgta ctccctggct catttccggt tttgctttgt cagggaaagc gatcatatca
     7861 accaattcaa tttgctgttg ctggctgcaa tctttaaacg atttttcaaa ccgttttata
     7921 cattgcaggt caagccagcg taaaccacct ttcattggaa cctggtgaga aggcatatct
     7981 tttacaataa actcaataaa ctcgggtact ttagcctcac tggcactacc acttatttca
     8041 tcttttggaa tgataatatc tgaaagtaaa gttatggttg ccatttcatg atccgtaaaa
     8101 aacctttctt tggccaggat ctctttctca tactttagct catcaggatt acgatccagg
     8161 ttaaattttg gttcttctgc ttttatagca tcagctttct tatcatcaga attgcagcct
     8221 gcaataactg cgggtactgc aacagccccc gtagctatca gtttaagtga ctttcttcta
     8281 tccatacaaa aagtttagtt ttttgtgatt ttgttcccag acgaaaaaaa ccaactgtta
     8341 cttttataga ttttgttttt tcatctcttc aataatatat tcagaagctc tcatactcaa
     8401 tgccagtatc gtccaggtaa tatttttatc tgcctggctt acaaactgtc ctccatccac
     8461 acaaaacagg tttttacaat catgtgcctg gctccattta ttgagtgcag atttttttgc
     8521 atcattaccc atacgaacgg tgcctgcttc atggatgatc tctccgggtt tagcaaggcc
     8581 ccattgattt ttttcatcat catcaccacc ccatgttata atcgctccca tattatgcat
     8641 tacctctttg aaggtttctt tcatatgttt agcctgttta acttcatgtt cactccattt
     8701 tacattgaac ttcagcacag ggataccaaa cttgtcaact acgtcgggat ctattgcgca
     8761 ataattactt tccaacggta ttgcttcgcc acgacctgcc atcccaacac ctgcaccgta
     8821 aaaaaaccga taatcttctt ttaaagaagc tccgtatccg cctgcttctt tttgttttcc
     8881 ttttactaga tacttaccgt tcaggttttc tattccccaa ctaaaaccat aggagggttg
     8941 actcattcca ccccaatatt caatatgata acctcttgga aaatcgagtt tcttgttatc
     9001 cagccaccag ggtgaataga tgtgcatacc acctacacca tcttcattat acctttttct
     9061 atcgaacaac tggggcaata caccccccaa tgccgcaccg gttgaatcat gcaggtattt
     9121 cccaactaca ttactactat tggctaagcc attcggatgc cgctgcgatt ttgaattaag
     9181 taatagcctg gcactttcac aggcactggc ggcaacaatc acaacccggc cttctacctg
     9241 gtattccatc atatcgactt tacttatata ggatactccg gttgccaaac cttcgctatt
     9301 agtcaacact tctcttgcca tggcgtttgc aacaacatca atatttcctg ttttcatggc
     9361 aggataaatc aggacgttag gggaggagaa gtcagccttt gccatgctgc aattgcggtt
     9421 acattggcca caaaagaaac aggcacctct gcccgcatca ttttcaatgg gttttgtaag
     9481 aatagacaaa cgggaaggga tcactttcac accagcctgt acggcagcat tttttataaa
     9541 taattcgtgt aacctgggtt tgggtggagg taggaaaaat ccgtcgggat cattttccag
     9601 cccttcattc gtgccaaaaa tgccaatcag tttatctacc cggtcataat aaggcttgat
     9661 atcgtcataa cctattggcc agtcatcacc cagtccatct atactttttc ttttaaaatc
     9721 ttttgggccg aaacgaagtg agatccttcc ccagtgattg gtgcgcccac ccagcatcct
     9781 tgcccgccac cacatccaat cagtaccggc ttctttggtg aaaggttctc cttccactga
     9841 ccagccacca tagcaagcat cgaaatcacc aaaaggacga agtttggtac tggcaccccg
     9901 gcggggtgag tcccacggat tttttaattg agtgatattt ttttgcgggt catacatata
     9961 acctgcttta atgatgcaaa cttttaatcc ggccttcgcc agcatataag cagc
//
LOCUS       DAORTI010000122         9857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7304_length_9857_cov_7.110590, whole genome shotgun sequence.
ACCESSION   DAORTI010000122 DAORTI010000000
VERSION     DAORTI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7304_length_9857_cov_7.110590"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLO70_13205"
     CDS             complement(<1..578)
                     /locus_tag="PLO70_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75471.1"
                     /translation="MKKITLLLLIAVSLFGCKSKKDIPDVSGIDVNIPIERFDISFFS
                     IDTNDISNSLQKVQEKHPDFYTDFMQNILGVSGADTSQSTLLVTKEFLRGYLPIYDSL
                     QPQYQKTDWLQKELKKAFQFVKYYYPQYKTGKAILFLGPFDAPGVATTNAGIAIGLQQ
                     YAGQNFSVYQSPMGQELFPSYISRRFAPEFITA"
     gene            657..>1178
                     /locus_tag="PLO70_13210"
     CDS             657..>1178
                     /locus_tag="PLO70_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQZ75472.1"
                     /translation="MRVALAQQNYHIGNFEENIRKIIEGINLAKQQGAELVVFSELCV
                     CGYPPRDFLEFEDFINKCYQSIDVIKAYADTIGVLIGAPDRNPVREGKDLFNAAYLLY
                     EREIKGVAHKTCLPNYDVFDEYRYFEPAYDWKIIEFKGKRLAVTICEDIWNLGDNPLY
                     RNCPMDELMKQKPD"
     assembly_gap    1179..1278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1279..2214
                     /gene="nadE"
                     /locus_tag="PLO70_13215"
     CDS             <1279..2214
                     /gene="nadE"
                     /locus_tag="PLO70_13215"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQZ75473.1"
                     /translation="LNDDGTFTLPVSVFSKDLPDSEFNPNELDYNLCTAEIYSAIIIG
                     IRDYFKKMGFTKAILGSSGGIDSAVTLALACEALGKENVRAVLMPSQYSTGHSVSDAE
                     QLSKSLGNPYDIIPIRNIYDSFLNELKPIFNDLPFSVAEENIQSRTRGNLLMAIANKF
                     GYILLNTSNKSELATGYGTLYGDMAGGLGVLGDCYKMQVYALAKYINRNKEIIPENII
                     TKPPSAELRPGQKDSDSLPDYAILDRILYQYIERRKGPKEIKSQGFEAALVDRVLRLV
                     NINEYKRNQFCPIIRISPKAFGVGRRVPIVGKYLS"
     gene            complement(2219..2674)
                     /locus_tag="PLO70_13220"
     CDS             complement(2219..2674)
                     /locus_tag="PLO70_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75474.1"
                     /translation="MQKQQLHLTLITFLTLFIFTGCDKNNDPAPKTKTELLTQASWKF
                     SSASAAGTDITDNPAIVCVKDDVVTFSANGSGTVTEGTVVCNPTTAGNITWNFQDSET
                     KLMMSSGLFPAGSGLFTIVTLNETTLTLSQDVTIPPSPIPVPVSATYVH"
     gene            2827..3825
                     /locus_tag="PLO70_13225"
     CDS             2827..3825
                     /locus_tag="PLO70_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQZ75475.1"
                     /translation="MLKTAEDIRQLNERIGHAGQFTDKLREEIGHVIVGQSHMLDRLL
                     MGLLSNGHVLLEGVPGLAKTLTIKSLAQAIHAKFSRIQFTPDLLPADVIGTMIYNQQR
                     NEFIVRKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGESTYKLDEPFLVLAT
                     QNPLEQEGTYPLPEAQVDRFIMKVIVDYPKMHEEQVILRQSVQGNFPDIKQVVSVQEV
                     LNARDLTKKIYMDEKIEQYILDIVFATRRPDEYKLEKLKPLISYGASPRGSINLGLAS
                     KAQAFLNKRGFVIPEDVRSICKDVLRHRIGLTYEAEAENVKVEDVIEDVLKQINVP"
     gene            3825..4697
                     /locus_tag="PLO70_13230"
     CDS             3825..4697
                     /locus_tag="PLO70_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQZ75476.1"
                     /translation="MLTTAEILKKVRELEIKSKKLTTDLFTGEYHSAFKGKGMSFKEV
                     REYAAGDDIRFIDWNVSARFGHPFSKVFEEERELTVMMLVDISNSSLFGTTYARKRDI
                     ITEIGAVLSFSAVNNADKIGVIFYSDVVEAYIPPKKGRQHALYIVRELLSKEPKGKGT
                     AVSKALRYFTNSTKQKSIAFVLSDFLDANYEDALRIAGKKHDVIGIRLYDKMDMELPA
                     AGLLQVQDAESAITKWVDSSNAFVRYSYQQEFFRVTDYATQTFKKAGCDLLHIKTGDD
                     YVKVLQRFFLSRNR"
     gene            4755..5594
                     /locus_tag="PLO70_13235"
     CDS             4755..5594
                     /locus_tag="PLO70_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQZ75477.1"
                     /translation="MLAATTVIAQSATVIKASVDRSSILIGEPLQLTIEVQIAANEPI
                     RFVSIDTIEHFEFLEKPRIDTADTDNGTTLKGIYKITSFDSGRWVIPSFNISGATKTD
                     TIPVDVLFSAFNPAQEYHDIKDIIEVTPEQKKDWWWYAAGGGVLILLLILYLLRKRKP
                     KPVVATPAVIINPYEEAINNLRELNGDYADVKKYHSRLTDIFRLYIFRKKGILSLQKT
                     TDDLVLQLKNLGISKVQYDQLAQSLRLGDFVKFAKYVPAEEDNRLAFETVKKTIDEIE
                     RLS"
     gene            5594..6607
                     /locus_tag="PLO70_13240"
     CDS             5594..6607
                     /locus_tag="PLO70_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQZ75478.1"
                     /translation="MLYEWIKHMDFAYPLAFGLFILIPLLIWWYIKKYNSSQPTIRVS
                     AVHAFRVSSWKNKFRHLPFILRLAVLSCLIIALARPQKRNDQQQTEGEGIDIVLCMDV
                     SGSMGSRDILPSRMEVAKEVAEEFVRSRPVDRIGLVIFSGESFTQCPITTDRNTLIGQ
                     IQSLESRRYLIDGTVIGEGLATAVDRLSSTEGKSKVIILLTDGKEDPPETRLIDPLTA
                     LEIAKTKKVKVYTIGMSAAASTVVENTGVKKGNKNPLTDFLDEELLNRIANETGGKYF
                     RAKDKDGLKSIYSQIDKLEKSKVEIQSFKRYEEKFLPFVLAALAFLFLELILKFTLFR
                     KFP"
     gene            6633..7559
                     /gene="rsgA"
                     /locus_tag="PLO70_13245"
     CDS             6633..7559
                     /gene="rsgA"
                     /locus_tag="PLO70_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQZ75479.1"
                     /translation="MKAIVYKSTGSWYSLKDEAGKFWNGRMKGVMKLDDITSTNPVAV
                     GDWVHAEIENEGDSTVTINEILDRNNYINRQSPRHKHQHHIVAANLDQSILVATLKEP
                     KTSQGFIDRFLVASEMYHVPAIIVFNKSDLYKAKELDKYEQWKDMYEAIGYKVLLVSA
                     EKNEGMDELMKVLKDKTTLVSGHSGVGKSSLLNVIFPNMNRKTQDVSGWSGKGQHTTT
                     FAEMFDLPFGGKIIDTPGMREFGLVDISKAELSHYFPEMRDRLNGCQFNNCLHVNEPG
                     CAIKEAVINKEIHEDRYVSYAGILDSIEEKNY"
     gene            complement(7563..7892)
                     /locus_tag="PLO70_13250"
     CDS             complement(7563..7892)
                     /locus_tag="PLO70_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3630 family protein"
                     /protein_id="HQZ75480.1"
                     /translation="MSLTLRTSLGCTEAIIADDAGLKQFYHVANVLSQDFKVKFSNKE
                     DDFDTINWEFRFKGHHMTLHYNIYNGISLFPTKTRDAVNKDNKAVVELAGVLEGRLFK
                     EEQRNIA"
     gene            complement(7964..8158)
                     /locus_tag="PLO70_13255"
     CDS             complement(7964..8158)
                     /locus_tag="PLO70_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75481.1"
                     /translation="MKNLRFFAVALSLMMLLSSCGMFSNLFKRKTGCPTNGKNVGAEK
                     ILSGDPDASKSAKKAKKFRS"
     gene            complement(8237..8776)
                     /locus_tag="PLO70_13260"
     CDS             complement(8237..8776)
                     /locus_tag="PLO70_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQZ75482.1"
                     /translation="MPVILPVEDKYPQFGNNCFIAPNATIVGNVVMGDDCSAWFNTVI
                     RGDVNFIKMGNKVNVQDGACIHCTFQKYGTTIGNNVSIGHNAIVHGCTLHDNILVGMG
                     AIIMDNCVVHSNTIIAAGAVVLENTICEAGSIYAGVPAKKVKDISQELVHGQIDRIAN
                     NYIRYADWFRNQAGSNDNK"
     gene            8885..>9857
                     /locus_tag="PLO70_13265"
     CDS             8885..>9857
                     /locus_tag="PLO70_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQZ75483.1"
                     /translation="MTQRELFLRHVAQTSSAPLALEIVKADCATLYDAAGKEYIDLIG
                     GISVANIGHRHPKVIEAIQKQLDAYLHIMVYGEFVEAPQVQYAKLLTEYLPSALNAVY
                     FTNSGAEAVEGAMKLAKRVTNRTQIIAFNHSYHGSTQGALSIIGDEYWRNAFRPLLPD
                     VLHLEYNSFASINEITAQTACVITETVQAEAGILAPTKEWMLALRKKCTETGTLLILD
                     EIQAGFGRTGKLWGFEHFDIVPDVLLLGKALGGGMPLGAFIADKKLMDAFTDNPVLGH
                     ITTFGGHPVCCAAGMAGMKALLEEGWIGTVKGKEELFTSLLVHPKIKA"
BASE COUNT         2997 a         1775 c         2207 g         2778 t
ORIGIN      
        1 gcagtaataa actcaggagc gaaacgcctt gaaatataag aagggaaaag ctcctgcccc
       61 ataggggatt ggtacacaga aaaattttga ccggcatatt gctgtagtcc aattgcaata
      121 ccagcattgg tagtggcaac tcccggtgca tcaaaagggc cgagaaataa aatagctttc
      181 ccggttttgt attgcgggta atagtatttt acaaactgga atgctttttt caattctttt
      241 tgcagccaat cggtcttttg atattggggt tgaagtgaat catatatggg caggtaacca
      301 cggagaaatt cctttgttac gagcaaagtg ctctggctgg tatccgcacc gctgacaccc
      361 agtatgtttt gcataaaatc agtataaaaa tcaggatgtt tttcctgaac tttttgcagg
      421 ctgttactga tgtcattagt atcaatacta aaaaaactga tatcaaatct ttcgatagga
      481 atattaacat caatacctga tacatcaggg atgtcttttt tgcttttaca tccaaaaaga
      541 gaaacagcaa tcaataaaag cagagtgatt tttttcatag ctcttgaaaa cgtaactggt
      601 ttaaaaatgt tgtgacacga atgtacgcta caaaaacatt agcttaaatt tgtttaatga
      661 gagtagcatt agcgcagcaa aattatcata ttggcaattt tgaagaaaat atccgaaaaa
      721 tcattgaggg gattaacctg gccaaacaac agggagcgga actggtggtg ttctcagagc
      781 tatgtgtttg tggttatccg ccaagagatt tcctggaatt tgaagatttt atcaataaat
      841 gctatcaatc aattgatgtt attaaggcct atgcagatac tatcggtgta ttaattggcg
      901 ccccggacag aaatcctgta cgggagggca aggacttgtt taatgcagcg tacctgctgt
      961 atgaaaggga aataaagggt gtggcacata agacctgcct gcctaattat gatgtgtttg
     1021 atgaataccg gtattttgag ccggcttatg attggaaaat aatcgagttt aaagggaaga
     1081 gattggctgt tactatctgt gaagacatct ggaatcttgg agataatccg ttataccgga
     1141 attgcccgat ggatgagctc atgaagcaaa aacctgacnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnct gaatgatgac ggaactttta ccctgccggt ttctgttttc
     1321 tcgaaagacc tgccggattc agaattcaat ccgaatgaac tggattataa tttatgtact
     1381 gctgaaatat acagtgcaat cattattggc atcagggatt attttaagaa aatgggtttt
     1441 accaaagcta ttcttggtag cagtggtgga atagatagtg ctgttacact ggcattagct
     1501 tgtgaagctt taggtaaaga aaatgtgaga gctgtgttga tgccttcaca gtattcaacc
     1561 gggcatagtg taagtgatgc tgagcagctg agtaaaagcc tgggaaatcc ttacgatata
     1621 atacctatca gaaatattta tgattcattt ttgaatgaat tgaaaccaat cttcaacgat
     1681 ttgccattta gtgttgctga agagaatatt caaagtcgta ccaggggtaa cctgttaatg
     1741 gctattgcca ataaattcgg ctatattttg ctgaatacat ccaataaaag tgaactggct
     1801 accggctatg gcactttgta tggagacatg gcgggggggc ttggcgtatt aggcgattgt
     1861 tacaaaatgc aggtctatgc gttggctaaa tacataaaca ggaacaaaga gatcattcct
     1921 gaaaatatta taaccaaacc tccttctgca gagttgcgtc ccggccaaaa ggactctgac
     1981 tcattgcctg attatgcgat actggacagg atattgtatc agtatattga aaggagaaag
     2041 gggccaaaag aaataaaatc acagggtttt gaagctgcgc ttgtagacag agttttgcgg
     2101 ttagtaaaca tcaatgaata caaacgaaac cagttttgcc cgataatacg catttcgccc
     2161 aaagcatttg gtgtgggcag aagagtacca attgtcggta aatatctttc ctgatttttt
     2221 aatggacata agtagctgaa accggcaccg ggataggtga aggggggatt gttacatcct
     2281 gtgataaggt caaggttgtt tcattgagag ttactattgt gaaaagccca gaacctgccg
     2341 gaaaaagtcc cgatgacatc atcaatttag tttcactatc ctgaaaattc caggtgatat
     2401 tgccagccgt ggttggatta caaaccaccg tcccttccgt taccgtaccg ctgccatttg
     2461 ctgaaaaggt cactacatcg tcttttacac agacgattgc aggattatcg gtaatatcag
     2521 ttcccgcagc ggaagcacta ctgaatttcc agcttgcctg ggttaataat tcagtctttg
     2581 ttttgggagc cgggtcgtta tttttatcac aaccggtgaa aataaataac gttagaaaag
     2641 ttatcagtgt caggtgtaat tgttgttttt gcataaatcg ttggattatt tgatcaaaat
     2701 actcaatttt tttggcttta tataggtaaa atactccaaa atagtgccgg aattcccttt
     2761 attcaaataa cggcaaatta tttgcataca gcaagttcgt attttgtgac tctataaaca
     2821 tttcccatgt taaaaaccgc agaagatatc cgccagctga atgaacgtat cggccatgca
     2881 ggacagttta ccgacaaatt aagagaagaa ataggccatg tcatcgtggg ccaaagtcat
     2941 atgcttgaca gattgttgat gggtttactc agcaatggac atgtattact cgaaggtgtt
     3001 cctggtttgg ccaagacgct aactatcaag tcactggcac aggctattca cgccaaattc
     3061 agccgaatac agtttacgcc ggatttattg ccagctgatg tgatcggtac aatgatctat
     3121 aaccagcagc gtaatgagtt tatcgttcgt aaagggccca ttttcgccaa ttttatcctg
     3181 gcagatgaaa taaacagggc acctgctaaa gtacaaagtg cattgctgga ggcgatgcag
     3241 gaaagacaag taacaattgg tgaatcaact tacaagctgg atgaaccatt cctggtattg
     3301 gccacacaaa atcctttgga gcaggaaggt acgtatccat taccggaagc acaggtggac
     3361 cgattcatta tgaaagtaat tgtagattat ccgaagatgc atgaggaaca ggtgatattg
     3421 cggcagagtg tacagggaaa tttcccggat attaagcagg tagtatctgt acaggaagtg
     3481 ctcaatgcaa gagacctgac taaaaagatt tacatggatg aaaaaattga acaatacatc
     3541 cttgatattg tttttgccac ccgccggcca gatgagtata aactggaaaa attaaaacct
     3601 ttgatttcct atggcgcttc tccaagagga agtatcaatt tagggttagc atcaaaagca
     3661 caggcatttt taaataagag aggttttgtg attccggaag atgtgcgtag tatttgtaaa
     3721 gatgttttgc ggcaccgtat cggattaacg tatgaagctg aagcagaaaa tgtaaaggtg
     3781 gaagatgtga ttgaggatgt tttgaaacaa attaatgttc cctgatgctg actactgcag
     3841 aaatattaaa aaaagtccgt gagctggaaa taaaaagcaa aaaactgacg actgacctgt
     3901 ttacaggaga atatcacagt gcttttaagg gtaaagggat gtcgttcaaa gaagtgagag
     3961 aatatgctgc cggtgatgac atacgtttta tagactggaa tgtgtcggca cggtttggtc
     4021 atccgttcag taaagtattc gaagaagaaa gagagttaac cgtgatgatg ttggttgata
     4081 tcagtaacag ttctttgttt ggaactacct atgcaagaaa gagagatatt ataacagaaa
     4141 ttggggcggt acttagtttt tcagctgtaa ataatgccga taagattggt gttatttttt
     4201 atagcgatgt ggtagaagcc tatattccgc ccaagaaagg aagacaacat gcattatata
     4261 ttgtaaggga attattgagc aaagagccaa aaggaaaggg caccgctgtc agtaaggcac
     4321 ttcgttattt tacgaactcc accaaacaga aaagcattgc atttgtgctg agcgatttcc
     4381 tggatgctaa ctatgaagat gcattacgaa ttgccggaaa aaagcatgat gtaattggta
     4441 tcaggttgta tgataaaatg gatatggaac tgccagcagc aggcctgctg caggtacagg
     4501 atgcagagag tgccataaca aaatgggtgg acagcagcaa tgcttttgtc aggtacagtt
     4561 atcagcagga atttttccgg gtgacagact atgctacaca gacttttaaa aaagccggtt
     4621 gcgacctgct gcatataaaa acgggtgatg attatgtaaa agtgctccaa cgatttttcc
     4681 tcagccgtaa ccgctgattc aatgatgtaa taattagaat gatttttcaa cgaacaatat
     4741 tttttctttt tttcatgctt gcagcaacta ctgtaattgc ccagtctgca actgtaataa
     4801 aggcttctgt tgatagatcc agcattttga tcggggaacc attacaactt actatagagg
     4861 ttcagattgc agccaatgaa ccgatacggt ttgtatccat tgataccatt gaacattttg
     4921 agtttctgga aaagccccgt atcgacacgg ctgatactga taatggaact accctgaaag
     4981 ggatctataa gatcaccagc ttcgactcag gccgttgggt tattccttct ttcaatattt
     5041 caggggcaac aaagacagat accattcccg ttgatgtgct attttctgct ttcaatcccg
     5101 cacaggagta ccatgatata aaagatatta ttgaggtaac tcctgaacag aaaaaggatt
     5161 ggtggtggta tgcagcgggt ggtggagtat tgatattatt acttatacta tacctgcttc
     5221 gaaaaaggaa accaaaacct gttgtggcca caccggcagt aatcatcaat ccatatgaag
     5281 aagctattaa taatctccgg gaacttaacg gagattatgc agatgttaaa aaatatcatt
     5341 ccaggctgac ggatattttc cggttatata ttttcaggaa aaaagggata ttgtcattac
     5401 agaaaactac tgatgatctt gtactgcagt taaagaatct gggtatcagt aaagtgcagt
     5461 atgatcagtt agcacaatca ctccggctgg gtgattttgt gaagtttgcc aaatatgtac
     5521 ctgcagaaga agataaccgg ctcgcttttg aaactgtaaa aaaaacaatt gacgaaatag
     5581 aaagattaag ctgatgctgt acgaatggat aaaacatatg gattttgctt acccgcttgc
     5641 atttggctta tttatcctga ttccgctttt gatatggtgg tacataaaga aatataacag
     5701 tagccaaccg acgatcaggg tttccgctgt tcatgctttt agggtctcct catggaaaaa
     5761 taagttcagg catttaccat tcatattacg gttagcagta ttgagttgtt taattatagc
     5821 actggcccgt ccgcaaaaaa gaaatgatca gcaacaaact gaaggagagg gaattgatat
     5881 cgtactttgt atggatgtaa gcggcagcat gggatcaaga gatatattgc catcaagaat
     5941 ggaagtggcg aaagaagttg cagaagaatt tgttcgcagc cgtccggttg acagaatcgg
     6001 gctggtgatt ttttcaggag agagttttac ccaatgtcct attactacag acaggaatac
     6061 attgattgga cagatccaat cgttggaaag ccgcaggtat ttgattgatg gtactgtaat
     6121 tggtgaagga ctggctacag cagtggaccg tctttccagt actgaaggga aaagtaaagt
     6181 aatcatttta ttgacagatg gaaaagaaga tccacccgaa acaagattaa ttgatcccct
     6241 tacagcactg gaaattgcca agactaaaaa agtaaaagta tacactattg ggatgagtgc
     6301 agctgcgtca acagtcgttg agaataccgg cgttaaaaag ggtaataaaa atccattgac
     6361 agattttctc gatgaagaat tgctaaacag gattgcgaat gagacagggg gtaaatattt
     6421 cagggctaaa gataaagatg gtctgaaaag catttacagc cagatagata aactggaaaa
     6481 atcaaaagtg gaaatacaat ctttcaaacg gtatgaggaa aaatttcttc cttttgtatt
     6541 agctgcgttg gcgtttcttt tcctcgagct gattttaaag tttactttat tcagaaaatt
     6601 tccctgagtg tttatcaatt atcttgcaac acatgaaagc gatagtgtat aagtctaccg
     6661 gtagttggta tagcctgaag gatgaggctg gcaagttctg gaatggacgg atgaaaggag
     6721 taatgaagct cgatgatatt accagtacca acccggtagc tgttggtgac tgggtacatg
     6781 ctgaaataga aaacgagggg gacagtacgg taacaatcaa tgagatactt gaccggaata
     6841 attatataaa caggcaatct ccccgccaca agcaccaaca tcatattgta gctgccaatc
     6901 tggatcaatc catcctggta gccactttaa aagaacctaa aacatcacag ggttttattg
     6961 accgtttcct ggtggcatca gaaatgtatc atgtgccggc gatcattgta tttaataaat
     7021 ctgatctata taaggctaag gaactggata aatatgaaca atggaaagat atgtatgaag
     7081 cgatcggtta caaagtactg ctggtgagtg ctgaaaagaa tgaagggatg gatgaattaa
     7141 tgaaggtatt gaaagataaa accacccttg tcagcgggca cagcggtgtg ggtaaatcat
     7201 ctttattaaa tgtgattttt cccaacatga accggaagac ccaggatgta agtggctgga
     7261 gtggtaaagg tcagcataca acaacttttg cagaaatgtt tgaccttcct tttggcggta
     7321 agatcattga tacacccggt atgagagaat ttgggctggt ggatatttca aaagcagaat
     7381 tgtctcacta ttttcccgaa atgcgggaca ggttaaatgg ctgccagttt aataattgcc
     7441 tgcatgtaaa tgaaccgggc tgtgccatca aagaggcagt aattaataaa gagatacacg
     7501 aagacaggta tgtgagttat gcaggcatac tcgactccat cgaagaaaag aattattaaa
     7561 gcttaagcga tgtttcgctg ttcttcctta aataatctgc cttccagtac accggctaat
     7621 tccactactg ctttattgtc tttatttaca gcatcacggg tcttggtggg gaaaagagaa
     7681 atcccgttgt agatattata gtgtaaagtc atatgatgac ccttaaaccg gaattcccag
     7741 tttatagtat caaaatcatc ttctttgttg gaaaacttta ctttgaaatc ctggctaagg
     7801 acattagcca catgatagaa ctgttttaat ccggcatcat ctgcgattat ggcttcagta
     7861 cagccaagtg aggttcgtag ggtaaggctc atagtattaa tgtttaaagg tttatatact
     7921 acaagaccga aatgggtgga ttttagctgc acataacagt aatttaactt cggaattttt
     7981 tggccttttt tgcgcttttc gacgcatcgg gatccccgct taagatcttc tctgcaccaa
     8041 cgtttttccc gtttgtagga cagcctgttt tacgcttaaa caggttgctg aacattccac
     8101 aggaagaaag caacatcata aggctcagtg caacagcaaa aaatcgtagg tttttcattt
     8161 tttcggttac ttgaataacg aaagtagata gaaataattg cacctgccat agtaaatcta
     8221 cccttccgga gatttactat ttgttgtcat tactaccggc ttgattacga aaccaatcgg
     8281 cataacggat ataattattg gcgatacggt ctatctggcc atgtaccagt tcctgcgaaa
     8341 tatctttgac tttttttgcg gggacccctg catagatact accggcttca catatagtat
     8401 tttctaatac gaccgccccg gcagctatga tcgtattact gtgcactaca caattatcca
     8461 taatgattgc tcccatgcct accagtatat tgtcatgcag ggtgcagcca tgcacaatag
     8521 cattatgtcc aatcgaaaca ttgttaccga tagttgtccc gtatttctga aaagtacaat
     8581 ggatgcaggc cccatcctgc acattcacct tattacccat tttaatgaaa ttcacatcgc
     8641 cccggatcac agtattgaac caagcactgc aatcatctcc catcactaca ttaccaacga
     8701 tagtggcatt aggagcgata aaacagttgt tgccgaattg gggatatttg tcttctacag
     8761 ggagtatgac cggcatatgc ttggttttag gtttgaagtt taatgtttga ggttagtttg
     8821 taaaattgag aaattctttg cgcctttgca cctcctttgc gttctttgtg gtaaataatt
     8881 tgtcttgacc caacgggaac tttttttacg gcatgtagcg cagacctcct ccgcaccgct
     8941 ggcactggag atcgtaaaag ccgattgtgc cactttgtat gatgctgccg gcaaggaata
     9001 cattgatctc atcgggggta tcagtgtggc caatatcggt caccggcatc caaaagtaat
     9061 tgaagctatt caaaaacagc tggatgctta tctgcacatc atggtttatg gcgaatttgt
     9121 agaagctccg caggtgcaat atgccaaact gctgacagag tacctgcctt cagctctcaa
     9181 cgctgtttat tttaccaact ccggtgcaga agcggtagaa ggggctatga aattagccaa
     9241 gcgggtcacc aaccgaacac agatcattgc ttttaatcat tcttaccatg gcagtaccca
     9301 gggtgcgttg agtattattg gtgatgaata ctggcggaat gcattccggc cgttgttgcc
     9361 ggatgtattg caccttgaat ataattcctt tgcatccatc aatgagataa cagcacaaac
     9421 agcctgtgta attaccgaaa ctgttcaggc tgaagcaggg atacttgcac caacgaaaga
     9481 atggatgctg gctttgcgga aaaaatgtac agaaaccgga acgcttttaa tactggacga
     9541 gattcaggcc ggctttggaa gaaccggtaa actttggggt tttgaacatt ttgatatcgt
     9601 gcccgatgta ttgttgcttg gtaaagcatt gggtggcggt atgccattgg gagctttcat
     9661 tgccgataaa aaactgatgg atgcttttac cgataatcct gtactggggc atatcacaac
     9721 ctttggtggc cacccggttt gctgtgctgc cggaatggcc ggtatgaaag cattgctgga
     9781 agaagggtgg attggtacag taaaaggcaa agaagaactg tttacctcgc tgttagttca
     9841 tccaaaaata aaagccg
//
LOCUS       DAORTI010000123         9685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7491_length_9685_cov_6.811215, whole genome shotgun sequence.
ACCESSION   DAORTI010000123 DAORTI010000000
VERSION     DAORTI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7491_length_9685_cov_6.811215"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLO70_13270"
     CDS             complement(<1..536)
                     /locus_tag="PLO70_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQZ75484.1"
                     /translation="MKNFRQQHRPKKSSLVIGRNAVIEVLRTGQQLDRIYLDTRATGE
                     DISEIKRLAVQNGVPVNYVPAAKLNSFNVTNHDGCVAQIAKVQYQNLQDVISLIIDNG
                     EAPLLLILDGITDIRNIGGIARTALCCGVHAIIIPDKGVGALNEDAILTSAGALEQIA
                     VCRVNSLMKAVAELHLSG"
     gene            complement(628..1884)
                     /gene="metK"
                     /locus_tag="PLO70_13275"
     CDS             complement(628..1884)
                     /gene="metK"
                     /locus_tag="PLO70_13275"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQZ75485.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAFDPQSKVACETL
                     VTTGQVILAGEVKSKAYLDVQEIARGVIRKIGYTKSEYMFEASSCGVLSAIHEQSSDI
                     NQGVDRKKKEEQGAGDQGMMFGYATNETEHYMPLALDLAHNLLIELAAIRREKNSKMP
                     YLRPDAKSQVTLEYDDNNQPVRIDAIVISTQHDDFDSETKMLAKIKNDVITILIPRIK
                     ARYKKYAKLFNSDIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGLASEVLVQVSYAIGVAQPTSINVNTYGTSKVQM
                     TDGEISKVVERIFDMRPYFIEQRFKLRNPIYSETAAYGHMGRKSEVVEKTFTSPDGKK
                     VTKKVELFTWEKLDYVSKVKKAFGLK"
     gene            complement(1990..3426)
                     /locus_tag="PLO70_13280"
     CDS             complement(1990..3426)
                     /locus_tag="PLO70_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HQZ75486.1"
                     /translation="MKNNKIALSIIAIVASVAIVFSGCKKINEATELGGGLIPPIDNI
                     NTFETFLDIETDNLLFADTTKVYFNDDLALGHISNDPEFGVTHADAYFNISSSNYFTY
                     PFINRDSVTIDSVILSLSYQGSYGDTNSFQTIRVFEIAQNAGFNDSTLYRYGNPDFLT
                     TGPELGAKTFQVKKLSDSIQFIRKKDTSKLANVIRIPLDNQLGVRLKGYDTTNTANGG
                     FRTDSIFKTLFRGLAIKSDNSGNALTYIASTDNIKTKLIVYFQVTKNGIKDTTSTEFY
                     HLTGGQANIVKRTQGGGWDSYLTNGTANDDKIYLQGFPGSYASLKIPGLDTFSNAVIH
                     RAEIVATPLRSTQDDIFVQPFGLFLDRINTTGDTASTFDIDMSLTNNFTAYTYDISSF
                     GGLLKSDSTYRFNISRYVQNIITTRNTNYKLRLYSPIRAFVYSPGYKAINQVYVTDRV
                     AYGRVVLAGGSYINPSKRMRLRLVYSKL"
     gene            complement(3458..4273)
                     /locus_tag="PLO70_13285"
     CDS             complement(3458..4273)
                     /locus_tag="PLO70_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQZ75487.1"
                     /translation="MSAKKRILFIANEMSPYLELTEFSEIVNKLAIKANDNNLEVRCI
                     MPRFGIINERRHRLHEVVRLSGINVSVDNDDMPLQIKVASLPSARLQVYFLDNEELFK
                     RKFVFHDENEKWFDDNDLRTIFFCKGALETVKKFGWPPDIIHCSGWMTGLIPAYLKTV
                     YKKEPVFAHSKIVFTIGQNTFKEKLGSGFIKKALIHASLKDKDLEVYKEGTNTAMFRG
                     GATFADAITFGAEKIEKKLVDEFTKVRGKKTLPYNPEGDLTDYLQLYSDLAGK"
     gene            4451..5302
                     /gene="panC"
                     /locus_tag="PLO70_13290"
     CDS             4451..5302
                     /gene="panC"
                     /locus_tag="PLO70_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQZ75488.1"
                     /translation="MIIFKKTRDLHKWLEAQRLAGISIGFVPTMGALHTGHLSLIESS
                     IKANPVTVCSIFVNPTQFNDPKDFEKYPITIEKDIELLANAGCDILFLPSVKEIYPDE
                     LSSSLKYDLGYLERIQEGKFRPGHFQGVCMVVHRLLEIVMPDNLYLGQKDYQQCMVIK
                     KLIALIGLSNAILVNICPTLREPDGLAMSSRNVRLNEDERIKATAIFRNLSFIKENLT
                     TGDLKGLKETALRNLSEAGFNVDYIEIADANDLSIIDDWDGQKKLVALAAAFMKEVRL
                     IDNILLN"
     gene            5362..6378
                     /locus_tag="PLO70_13295"
     CDS             5362..6378
                     /locus_tag="PLO70_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQZ75489.1"
                     /translation="MNKRFRTILQYLLFLGLGIFFAWLSLKNLNKENTEQIKLALKNA
                     RYWLVFPVFLILVGSHLVRALRWRLLIEPLGYKPSVKNSFFAVMIGYLVNLGVPRLGE
                     IVKCTVLARYEKIPADKLIGTIILERMIDAITLLIIFGITLAIQPQLYSQIVEAYFSN
                     SSTGREKETSSFLITIILIAAIILLTGLWMVIKKKTIHDLFRLFKRIWKSVIEGISSI
                     RHLKKRKQFIVLSIVLWALYLSGGYIGFMAFRETEHYGIKEAFTILSAGSIGMIASPG
                     GIGAYAYLIEKTMELYGLKYTIALAFGWILWLAQTLVMILGGLISFVALPFFNKKKIT
                     SEQS"
     gene            6365..6856
                     /gene="rfaE2"
                     /locus_tag="PLO70_13300"
     CDS             6365..6856
                     /gene="rfaE2"
                     /locus_tag="PLO70_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQZ75490.1"
                     /translation="MNRADLIPQKILTAEQAVQKTAQWRILGKKISFTNGVFDILHQG
                     HIFSLSKAAKEGDYLVVGLNADVSVKRLKGESRPVNDQDSRALILASLLMVDAVVLFE
                     EDTPLELIKAILPDVLVKGGDYTVEQIAGAKEVIKNGGKVVINPILEGFSTTSLIKQM
                     KNH"
     gene            6938..9034
                     /gene="ppk1"
                     /locus_tag="PLO70_13305"
     CDS             6938..9034
                     /gene="ppk1"
                     /locus_tag="PLO70_13305"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQZ75491.1"
                     /translation="MAHIPKRKTIARDISWLSFNARVLQEAADPTVPLRERIRFLGIF
                     SNNMDEFFRVRVATLKRMIQFGHKANMHLENNPQQIIDEIQMTVLNQQGEFSRIWEEV
                     LLELKAQKIFIRNEKELNIEQQNFVRNYFEEEVSSEVIPLMIENIPVFPNLRDKSIYL
                     AVVMWKRETALKKKYALIEIPTRTLGRFKILPSSKTDEHHIILLEDVIRFNLPDIFSY
                     FGYDQYQSHIFKVTRDAEIDIDEDVSTNIIQKLEKGIKNRRKGKPVRFVYDREMDPGL
                     LEYLVRRLNLTKRDNLIPGGRIHNFRHFMDFPDQVFKEKRHRKKPFDHPLLTERRVTD
                     VMLEKDVLLHFPYHSFLPLIDLIREAAFDPEVTTIKITCYRLAQQSKIVNALINAVRN
                     GKEVVVMLELRARFEEEANIEWKNRLEDEGAKVLVEIPNMKVHSKICVIKKRTKDNST
                     LLYGFVSTGNLNERTAKVYGDHCLLTSNQKIMADVNRIFNFLEQPKTGMNFLRDCKIV
                     IPSPLFVKREMIRLIDEEIKQARKKKYAAITLKMNSLSDEMMIEKLYEAARAGVQLKL
                     IIRGIFCMISENKKFKKSVKAISIVDEYLEHARVWVFHNKGKEKVYISSADWMLRNLE
                     HRVEATCPIWNEELKQELKDILNIQLEDNVKARWLDNELNNEYVRTTKKKIRSQVETY
                     NYLHKKTLIHRETGSN"
     gene            9015..>9685
                     /locus_tag="PLO70_13310"
     CDS             9015..>9685
                     /locus_tag="PLO70_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQZ75492.1"
                     /translation="MRLAAIDIGSNAARLLITDVISGPQGVPEFIKTALVRVPLRLGF
                     DVFDKGEISAIKMEKILKTIKSYKLLLDVYEVRHLKACATSAMRDASNAPEIIKKVKA
                     ETGIEIQIISGDSEASLIYENHVAESMTRDESYLYVDVGGGSTELTFFSDGKLIFKKS
                     FNIGTIRLLKNQVNEAIWDEMKEFIRTKTKGFHHVTAIGSGGNINKIFSLSKRKEGKP
                     LSLEL"
BASE COUNT         2985 a         1912 c         1938 g         2850 t
ORIGIN      
        1 atgccactca agtgtaattc agccaccgcc ttcatcaaac tgttaacacg gcatactgca
       61 atctgttcta aagcacctgc tgatgtcagt atcgcatctt cattcaaagc ccctactcct
      121 ttatccggga taatgatcgc atgcacacca caacaaagtg cagttcgggc tattccgcct
      181 atattgcgaa tatcagtaat gccatcaagt atcagcaata atggtgcttc gccattgtca
      241 atgatcagag aaataacatc ctgcagattc tggtactgca ctttagcgat ctgcgccaca
      301 caaccatcat gattggttac attgaagcta ttcagttttg cagccggtac ataattgaca
      361 ggcactccgt tttgaacagc tagtcttttt atttcactaa tgtcttcacc agtggctcta
      421 gtatcaagat agatcctgtc taattgctga cccgttctca atacttctat aacggcattt
      481 cttccgatca ctaatgaact ctttttgggg cggtgctgtt gtctgaaatt tttcacttgt
      541 caaatgtatt ataaactaaa aaccctgcaa gtttttccaa ccagcagggc cttaaaaatt
      601 ttccttttaa ggaagatttg gagaggttta ttttaaccca aatgcttttt tcacttttga
      661 aacataatcc agtttctccc atgtaaacaa ttcaaccttt ttggtcactt ttttcccgtc
      721 aggtgaagta aatgtttttt caaccacttc agattttcta cccatgtgac cgtacgctgc
      781 tgtttcacta tatataggat tacgaagttt aaaccgttgc tcaataaaat aaggtcgcat
      841 atcgaaaata cgctctacca ccttactgat ttcaccatca gtcatttgga ctttggatgt
      901 accgtatgta tttacattga tactggttgg ctgcgccaca ccaattgcat aagaaacttg
      961 taccagcact tcactggcca atccggctgc caccaggttc ttggcgatat gtcttgttgc
     1021 ataggctgct gaacggtcta ctttggaagg atctttccca ctgaatgcac ccccaccgtg
     1081 agcaccttta ccaccgtaag tatctacaat gatcttacga cccgtcaaac cagtgtcacc
     1141 atgtggcccc ccgatcacaa attttccggt tgggttaatg tgatatttga tatcggaatt
     1201 aaacaactta gcatatttct tataccgggc ttttattctt ggtattagaa tagtgataac
     1261 atcatttttg atcttagcca gcattttggt ttcgctgtca aagtcatcat gctgggttga
     1321 aattacaata gcgtcgatac ggacaggttg attattatcg tcatactcca gcgtaacctg
     1381 actttttgca tcaggacgca ggtaaggcat tttagaattt ttttctctgc gaatagcagc
     1441 taattcaatc aacagattat gcgccagatc caatgcaaga ggcatataat gctctgtttc
     1501 attagtggca taaccaaaca tcatcccctg gtcaccggcc ccctgctctt ctttcttttt
     1561 cctgtcaact ccctggttaa tgtcggatga ttgttcgtga atggcagaca acacaccaca
     1621 gctgctggct tcaaacatat attcactctt ggtatatcct attttacgga taacaccacg
     1681 ggctatttcc tgcacatcca aataagcttt tgattttact tcaccggcta aaataacctg
     1741 cccggttgtt accagggttt cacaggcaac ttttgactga gggtcaaaag caagaaaatt
     1801 gtcgattaaa gcatcagata tctggtcagc aactttatcc ggatgacctt cactaactga
     1861 ctctgatgta aataaataag gcatgattaa tatgttttta aggacgcaaa gataagggta
     1921 cagctataaa agaacaaagt ccttcagcct gaagcagaag gacttttaaa catatcttaa
     1981 taacccaatt taaagtttag aataaacaag cctcaaacgc atccttttgg agggattaat
     2041 ataactgcct ccggcaagaa ctactcttcc ataagccacc ctgtcggtta catatacctg
     2101 gttaatggct ttataaccgg gagaatatac gaatgccctt ataggggagt atagccgtag
     2161 tttataatta gtatttcggg ttgtaatgat gttctgaaca tatcttgaaa tattgaaacg
     2221 ataggtgctg tctgacttta aaagcccgcc aaaagaagat atatcatagg tataagccgt
     2281 aaaattattg gtaaggctca tatcaatatc aaaagtggaa gcagtatcac cagtagtatt
     2341 gatcctgtcc agaaaaaggc caaagggttg gacgaaaata tcgtcctggg ttgagcgtag
     2401 aggggtagct acaatctcag ccctgtgtat aaccgcattg ctaaaagtat ctaacccggg
     2461 aattttcaat gaagcatatg agccgggaaa tccctgtaag tatattttat catcgtttgc
     2521 tgtgccattg gtaagatatg aatcccatcc gccaccctga gttcttttca caatattagc
     2581 ctggccaccg gtgagatgat aaaactctgt agaggttgtg tccttaatac cattcttcgt
     2641 tacctggaaa taaacaatca gcttggtttt aatattatca gtagaggcaa tataggtaag
     2701 cgcattgccc gaattatccg actttattgc caatcccctg aatagggttt tgaaaataga
     2761 atcagttctg aaaccaccgt ttgctgtatt tgtggtatca taccctttta gcctcacacc
     2821 cagttgatta tccaacggaa ttcgaattac attagccaat tttgaggtat ctttttttct
     2881 gataaactgt atcgaatcgc ttaatttttt tacctggaat gtctttgcac caagttccgg
     2941 gccagttgtc aggaaatcag ggttcccata tctataaagg gtactatcat taaacccggc
     3001 attctgcgct atttcaaaaa ccctgatcgt ttgaaaacta ttggtatcgc cataggatcc
     3061 ctggtatgat aatgaaagta ttacagaatc gattgtaaca gaatctctgt tgatgaaggg
     3121 gtatgtaaaa tagttacttg aactgatatt aaaataggca tctgcatggg taaccccaaa
     3181 ttccggatcg ttactgatat gtcccaaagc caggtcatca ttaaagtaaa ctttggtcgt
     3241 atcagcaaaa agcaggttat cagtttctat atccaggaaa gtttcaaacg tattgatatt
     3301 atcaataggc gggataagtc caccccctaa ttctgtagct tcatttatct ttttacaacc
     3361 ggaaaataca atagcaaccg aggctacgat tgctattatt gataaggcga ttttattgtt
     3421 cttcacaaag taaatttata atcggtgtta gtgattttta tttgcctgca aggtcgctat
     3481 agagttgtaa atagtctgtt aaatctcctt caggattata gggcaaggtt ttctttcctc
     3541 tcactttggt aaattcatcg accagttttt tttctatttt ttcagcgccg aaagtaattg
     3601 catcggcaaa tgttgcccca ccgcgaaaca tagcagtatt tgtaccttct ttgtaaactt
     3661 caaggtcttt atctttcaat gaagcatgga tcagcgcttt tttgataaag ccactaccca
     3721 atttttcttt aaaagtattt tgccctatcg taaagacaat tttgctatgg gcaaaaaccg
     3781 gttccttttt gtacacggtt ttcaggtaag caggtattaa tcccgtcatc cagccgctac
     3841 aatgaataat atccggcggc cagccaaact ttttaactgt ttccagggca cctttgcaga
     3901 agaaaatggt acgaaggtca ttgtcatcaa accatttttc gttttcgtca tgaaaaacga
     3961 attttcgctt gaataactct tcattatcca ggaaatacac ctgcaacctt gcactgggaa
     4021 gagaagccac cttgatttgc aatggcatgt catcattatc cacagatacg ttgataccag
     4081 ataatctcac cacttcatga agtctgtgac ggcgttcatt aataataccg aatcttggca
     4141 ttatgcaccg aacctccaga ttgttgtcat tagctttaat cgccagttta ttcacgatct
     4201 ctgaaaactc tgtcaactcc aggtagggcg acatttcgtt ggcgataaat aaaattcttt
     4261 tctttgctga catgggcgtc cagtttaatt taccactttt atgaaaaaga ggtacccttt
     4321 tcgctgctaa caggccactg cttttggtgg caataaaggc cggtagaacg gttgaaaggc
     4381 gggcaaaggt aattaattta agccgaattt gagtgtttga aagatattaa ggcacaaact
     4441 gattaagaat atgatcattt tcaaaaaaac aagagacttg cataaatggc ttgaggcgca
     4501 acgcctggca ggaattagta taggttttgt acctacaatg ggagctttac atactggtca
     4561 tttatcacta attgaatctt ccataaaggc aaacccggta acagtttgca gcatttttgt
     4621 aaatcccacc cagtttaatg acccaaaaga ttttgaaaaa tatccaatta ccattgaaaa
     4681 ggatattgaa ttacttgcaa atgcgggttg tgacattctt tttctccctt ctgttaaaga
     4741 aatatatcct gatgaactct catcatcttt gaagtatgac cttgggtatc ttgaaagaat
     4801 acaggagggg aaatttcgcc ccggtcattt ccagggtgtt tgtatggtag ttcaccgtct
     4861 gcttgaaata gtaatgccag ataatcttta tctcggccaa aaagactacc agcaatgtat
     4921 ggtcatcaaa aagctcattg cgttgatagg attaagtaat gcaattttag tgaacatctg
     4981 ccccactctt cgtgagccgg atggccttgc catgagtagc cgtaatgtca gactaaacga
     5041 agatgaaaga ataaaagcta ccgcgatttt tcgcaacctg agcttcatca aagaaaatct
     5101 tacaactgga gatctaaaag gcttgaaaga aacagcatta aggaaccttt cagaagctgg
     5161 gttcaacgtt gattatattg aaatcgctga tgcaaatgat ctttcaataa ttgatgattg
     5221 ggatgggcaa aaaaaattgg ttgcccttgc agcagcattt atgaaagagg tcaggcttat
     5281 tgataatatt ttactgaatt aacggttaaa tgacacctac cgttttgttt ttcagttaca
     5341 tcgctttact ttcggtaccg aatgaataag cgtttccgca ctatacttca gtaccttctt
     5401 ttccttggtt tgggcatttt ctttgcctgg ctgtcactaa aaaatttaaa taaggaaaat
     5461 actgagcaaa taaaactggc gttgaaaaat gcaagatatt ggctggtgtt cccggttttt
     5521 cttattcttg ttggaagcca cctggtaagg gctttgcgct ggagattatt aatagaacca
     5581 ctcggctata aaccctctgt aaagaattct ttctttgctg tcatgattgg ctaccttgtt
     5641 aatctgggag ttcccaggtt aggcgaaata gtgaagtgta cggttttagc ccgttatgaa
     5701 aaaattccgg cagataaact tattggaact attattttag agcggatgat cgatgccatc
     5761 acacttctta tcatatttgg catcacgctg gcaatacaac cccagttata cagccagatt
     5821 gtcgaagcat acttcagtaa ttcatcaaca ggccgggaaa aagaaacctc ctcttttttg
     5881 attaccatta tcctgattgc agcaataata ctactaactg gtttgtggat ggttataaaa
     5941 aagaagacga tccatgatct tttcaggcta tttaaacgga tttggaaaag cgtcatcgaa
     6001 ggtataagca gtatcaggca tttaaaaaag agaaaacagt ttattgtttt atcaattgta
     6061 ctgtgggcac tctatttatc cggtggttat attggattca tggcattccg ggaaactgaa
     6121 cattatggaa tcaaagaagc attcacgatc ctttctgcag gaagcattgg tatgatcgca
     6181 tcaccgggtg gtattggagc ttacgcctac ctgatcgaaa aaaccatgga gttatatggc
     6241 ctcaagtaca caattgcatt ggcttttggc tggatattat ggcttgcaca gacattggta
     6301 atgatactcg gtggtttgat tagctttgta gcattgccat ttttcaataa aaagaaaatc
     6361 accagtgaac agagctgatc ttataccgca aaaaatctta acagccgaac aggcagtaca
     6421 aaagactgct cagtggagaa tattgggtaa aaaaatctca tttaccaatg gggtttttga
     6481 tatcctacac cagggacata tattttccct gtcaaaggca gccaaggaag gtgattacct
     6541 ggttgtgggg ctaaatgctg atgtatcagt gaaaagatta aaaggggaaa gccggcctgt
     6601 taatgaccag gattcaagag cccttattct tgcatcgctg ttaatggtgg atgcagttgt
     6661 attatttgaa gaagatacac cacttgaact catcaaagct atactgccgg atgtattagt
     6721 aaaaggaggt gattatacag ttgagcaaat tgccggggcc aaagaagtca taaaaaatgg
     6781 agggaaagtt gttatcaatc ccattctcga agggttttct acaacatcat taataaaaca
     6841 gatgaagaat cactgatttt cgggttccta aaaacttaat attttacagc attactgttt
     6901 gataacttta tcctttatct ggaaataata aacccgcatg gcacatatac ccaaaagaaa
     6961 aacaatagcc cgggatatca gctggctatc ttttaatgca agagttttac aggaagctgc
     7021 cgacccaact gtgccccttc gtgaacgaat caggttcctg ggcattttct caaacaatat
     7081 ggatgaattt tttcgggtca gagtagccac attgaaacgg atgattcaat ttggccacaa
     7141 agctaatatg catctcgaga ataatccgca gcagatcatt gatgaaatac aaatgacggt
     7201 gctcaaccag cagggggaat tcagcaggat atgggaagaa gttctgctag aattgaaggc
     7261 acagaaaata tttatcagaa atgagaaaga attgaatatt gagcagcaaa attttgtaag
     7321 aaattacttt gaagaagaag taagctctga agtaattccg ttaatgattg aaaatatccc
     7381 tgtatttccc aatctccggg ataaatctat ttacctggct gtggtaatgt ggaaaaggga
     7441 aactgcttta aaaaagaagt atgcgctgat tgaaatacca acccgtaccc tgggacgttt
     7501 taagatacta ccctcttcaa aaacggatga gcaccatatt atattactcg aagatgttat
     7561 ccggtttaac ctgcctgaca ttttctcata ctttggttac gatcagtacc agtcacatat
     7621 ttttaaagtt acccgggatg ccgagattga tatagatgag gatgtttcta ctaatattat
     7681 tcaaaaatta gaaaaaggga taaagaaccg cagaaaagga aaaccagtcc gcttcgtata
     7741 tgacagggaa atggatccgg gcctgctgga gtatcttgtc cggcgtctga atcttaccaa
     7801 aagggataac cttataccgg gcggccgcat acacaacttc cgtcatttta tggatttccc
     7861 ggaccaggta ttcaaagaaa agagacaccg gaagaaacct tttgatcatc ccctgttgac
     7921 agagcgacgt gtaacggatg taatgctcga gaaagatgtg ctgcttcatt tcccgtatca
     7981 ttcctttctg ccactgatag accttattcg ggaagctgca tttgaccctg aggtgactac
     8041 tattaaaata acctgttacc ggcttgcaca gcaatcaaaa atcgtcaatg ccctgattaa
     8101 tgcggtgagg aatggcaaag aagtagtggt aatgctggaa ctaagggcaa gatttgaaga
     8161 ggaagcaaat attgaatgga aaaacaggct tgaagatgaa ggcgctaaag tgttggtgga
     8221 aatccccaat atgaaagtcc attccaagat atgtgtcatc aagaaaagaa ctaaagacaa
     8281 ttccaccctg ctctatggct ttgtaagcac cggtaacctg aatgaaagga cagctaaagt
     8341 atatggtgac cattgtttac ttacttccaa ccagaaaatc atggctgatg taaaccgaat
     8401 attcaatttt ctggaacaac caaaaaccgg gatgaatttt ctcagggatt gtaaaattgt
     8461 aatccccagt cctctttttg taaaaagaga aatgattcgc ctgattgatg aagaaataaa
     8521 acaagcccga aagaaaaagt atgccgccat tactttaaaa atgaactcac tttcggatga
     8581 aatgatgata gaaaaactgt atgaagctgc cagagccggt gtgcaactca agctaattat
     8641 ccggggcatc ttttgtatga tctcagaaaa taagaaattc aaaaaatccg tcaaggctat
     8701 tagcatagta gatgaatacc tggagcatgc aagagtgtgg gtatttcata ataaaggcaa
     8761 agagaaagtt tacatatctt cggctgactg gatgctcaga aatctggaac accgggttga
     8821 agctacctgc cctatctgga atgaagaatt gaagcaggaa ttaaaagata tactgaacat
     8881 acaattggaa gataatgtga aagcccggtg gctggacaat gagttgaata acgaatatgt
     8941 gcggaccaca aaaaagaaaa tccgatcaca ggtagaaaca tataactacc tgcataaaaa
     9001 aacattgatc caccgtgaga ctggcagcaa ttgatattgg aagtaatgca gcaagattat
     9061 taataaccga cgtaatatcc gggccccagg gtgtccctga atttatcaag acggcacttg
     9121 tccgtgtacc tttacggctg ggttttgacg tatttgataa aggtgaaatc tcggctatta
     9181 agatggaaaa aattctcaaa accatcaaat cgtataaatt gttgctggat gtttatgaag
     9241 tcaggcatct gaaagcctgt gccacatcgg ccatgcggga tgcatctaat gccccggaga
     9301 tcatcaaaaa agtaaaagca gagactggta ttgaaataca gatcatcagc ggtgacagtg
     9361 aagcatccct tatttatgag aatcatgtcg cagagagcat gacccgtgat gaatcctact
     9421 tatatgttga tgtaggcggt ggcagcacag agcttacttt tttcagcgat ggtaaactta
     9481 tttttaagaa atcatttaac atcggcacta tccgattact gaaaaaccag gtgaatgaag
     9541 ctatctggga tgagatgaag gaattcatcc gtaccaaaac aaaaggattc catcatgtta
     9601 cggccatagg ttctggtggc aatatcaaca agatattttc tctgtcgaaa agaaaagagg
     9661 gaaaaccttt atctcttgaa ctgtt
//
LOCUS       DAORTI010000124         9653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7519_length_9653_cov_8.333924, whole genome shotgun sequence.
ACCESSION   DAORTI010000124 DAORTI010000000
VERSION     DAORTI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7519_length_9653_cov_8.333924"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLO70_13315"
     CDS             <1..1071
                     /locus_tag="PLO70_13315"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HQZ75493.1"
                     /translation="PIFQTSTYVQEAPGVNKGFEYARSQNPTRKALEEALAIIENGKF
                     GLAFSSGVAATDAVIKLLAPGDEVICANDMYGGTYRLFTKVFEKFGIVFTYVDTTSVS
                     NVQAAITDKTKLVWLETPTNPLMNITDIASVAAITKAKKILLAVDNTFASPYLQNPLD
                     LGADIVMHSVTKYLGGHSDVIQGCLVMNDAELREKLYFIQKSCGAVPGPMDCFLVLRG
                     IKTLGVRMKAHCENGAKIANWLRSNPKVGKVYWPGFEDHPGHAVARKQMRGFGGMISF
                     ELKNDSVEEAKRVLSSTHLFSLAESLGGVESLINHPASMTHASIPREERIKNGLSDSL
                     IRLSVGIEDADDLIQDLQKAIG"
     gene            1079..1840
                     /locus_tag="PLO70_13320"
     CDS             1079..1840
                     /locus_tag="PLO70_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQZ75494.1"
                     /translation="MKERNLKEFLDKKVDDYNQPSFIKDDPISVPHLFTKKQDIEIAG
                     FFAAIFAWGNRTTIINKATELMQLMDNAPYEFCLTHDPGSLKKLMRFKHRTFNTTDLL
                     YFIEFFKFHYCRHKSLETAFTRHGSTIEEMLSGFHHYFFSLEEVPGRTKKHIATPERK
                     SSCKRLNMYLRWMVRKDKKGVDFGIWKNISPSQLICPLDLHVVRVAKRLNLLQRKQTD
                     WQAAVELTEYLRTLDKHDPVKYDFALFGMGVMEKY"
     gene            1846..2175
                     /locus_tag="PLO70_13325"
     CDS             1846..2175
                     /locus_tag="PLO70_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75495.1"
                     /translation="MTQVNTALVPEINQSLEIQLPLTISGEELREKLASHVNELINHD
                     FEKLVFNLYRIDVNEARMRALLAQKGGENAAALIADLIIERQLQKIKSRQQFRQRDNS
                     INENEKW"
     gene            complement(2178..2846)
                     /locus_tag="PLO70_13330"
     CDS             complement(2178..2846)
                     /locus_tag="PLO70_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQZ75496.1"
                     /translation="MEFEKIHNKGQARLFKNDFLEALTKAHPLVIWGMYIPILTYIIY
                     LAATKYNYSTGTILLVFFGGMFFWTLFEYIAHRYIFHWVPKTPGATKFVYTLHGNHHH
                     YPRDKQRLFMPPVPSLIISSTIFGLMYLAIGTYTFMFFPGFLLGYLMYGTMHYAIHAW
                     NPPFKWMKGLWRNHHLHHYKNEHNGYGVSSTLWDHIFGTMFDLKKEKEDKEKVKELMF
                     EKKK"
     gene            complement(2921..3574)
                     /locus_tag="PLO70_13335"
     CDS             complement(2921..3574)
                     /locus_tag="PLO70_13335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ75497.1"
                     /translation="MALVFTGHEFADGGDFIVRTLQQEKIKASFFFTGDFFRNPAFTK
                     IIVELKNNRHYIAPHSDKHLLYCDWGKRDSLLVTEAQFTKDLQRNYSMMSRFGIREEN
                     APFFLPPYEWYNDSIAVWTKKMDFQLINFTPGTLSHADYTTPADKNYRSSEDIYQSII
                     KYEQARPGGLNGFILLSHIGTDPKRTDKFYKRLPQLIKHLQAKGYQFQRVDELLKAD"
     gene            complement(3646..>3963)
                     /locus_tag="PLO70_13340"
     CDS             complement(3646..>3963)
                     /locus_tag="PLO70_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HQZ75498.1"
                     /translation="PWGTSMVYGLPSWGDTPVDPHSAFTHIKNYPIDGGLIDGPVYTS
                     IYKGLIGITLYQPDEYAEFQSDLAVYHDDYGDYSTNEPTMDGTASLIYLLAAKEAGSK
                     PKK"
     assembly_gap    3964..4063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4064..5296)
                     /locus_tag="PLO70_13345"
     CDS             complement(<4064..5296)
                     /locus_tag="PLO70_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HQZ75499.1"
                     /translation="MKKWSLLLLLLPVASFAQQPQSWIRINQLGYTPGAVKVATWCSK
                     EEKEIVKWELVNSVSKKIVATGMAGKAFGAYGPFKQTYRLNFSHFKKPGRYYLLAGNI
                     KSPEFEIGDDVYKGAADFCLRYMRQQRTGFNPYLKDSCHTHDGYVLYGEKAGIKDSTR
                     IDVVGGWHDASDYLQYSATSANATYHLLMAYRDFPKVFTDEKQANGLDGKNGMADVLD
                     EAKWGLDWLLKMHPRPDWMFNQIADDRDHMGMRIPGQDTFYGKGFERPVYFVSGEPQQ
                     RGRFMNNTTGTSSTAGKFASAFALGSKTFEKTNIEYSNLLLQKTFSAFAFANKVPGVT
                     QTASVKSPYIYAEDNWIDDMELANAYVLSKDNMPVHKEIIKKTLMDYANAEPITPWLG
                     ADTAKHYQWYPFINLGHYE"
     gene            5350..6354
                     /locus_tag="PLO70_13350"
     CDS             5350..6354
                     /locus_tag="PLO70_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQZ75500.1"
                     /translation="MKLVTYLKEEREQLGILINDMVYDMEVLHPDLPNSMNMLLNYWE
                     EYYPIAQAGELLLKEGTRTSNKAVPLTEVQLLAPVPFPTSCRDGYAFRQHVAAARRNR
                     KVEMIPEFDQYPIFYFTNHHSIQGPGDIRCMPDHFEKLDFELEAAIVICKHGRNIRAE
                     EADEYIGGLMIMNDISARRLQMEEMLLNLGPAKGKDFSTAIGPWLVTLDELAPFEIPA
                     KENHTGKSWNLRMQCRVNGKQVSDGNVGDMDWTYAEIIERASYGVDLYPGDVIGSGTV
                     GTGCFLELNGTGKLNDPNYPEQWLQPNDVVEMEIDGLGKLSNTIVAEDTDWSILKLKK
                     "
     gene            6437..7318
                     /locus_tag="PLO70_13355"
     CDS             6437..7318
                     /locus_tag="PLO70_13355"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQZ75501.1"
                     /translation="MIIDLKDLKPAEKQYYLQHVVAPRPICFASTINKAGHINLSPFS
                     FFNLFSSNPPIVVFSPARRVRDNTTKHTLQNVLEVPEVVINIVTYDMVQQTSLASCEF
                     PKDVNEFTKAGFTQEPATMIKPPMVKESKVKMECKVVEVKSLGNEGGAGNLVICEVIR
                     MHIDDSLLDENKKLDQRKINHVARLGGDWYCVVNEHNLFQVEKPNTQLGIGIDMLPAT
                     IRNSKILSGNNLGQLANVHEMPVIEPSFDDNHLKQIIQYYSVNPDEMEKELHSYAKKL
                     LEEDKVKEAWQVLLAGS"
     gene            7406..8299
                     /locus_tag="PLO70_13360"
     CDS             7406..8299
                     /locus_tag="PLO70_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75502.1"
                     /translation="MLKAFPSYLLLTLVVIITLFSCVPQKGVTNAKKDLATINNQLQQ
                     HSTTLKDLEKQRKKKEQQNEIDDTANTRIKKFIDKTNKEIDTLINRNTIIIGETVVEK
                     SDWDRLRKTLSFSRKTSKNIGDRILLLSDLINRNMVVRIDQDVVFEPGKYNITPEVAA
                     AIGRLFEPASKEIDLFVKKYPNFPLSLVITAKGYSDATSIAEGSSLYKELKDRLKLQT
                     ENPTSKELNKELSVARAEAVINLFKSYTLDRGSNGGSVSNILYLHEGKGETSPDPKVT
                     DYKINDARRRVVLLFWSVFPE"
     gene            complement(8303..9016)
                     /locus_tag="PLO70_13365"
     CDS             complement(8303..9016)
                     /locus_tag="PLO70_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQZ75503.1"
                     /translation="MKTSFIVIAFSLIVLPAGSFAQIKLKDVLNKKSSSGITENEAGQ
                     GIKEALTNGVTTAVLNLNKTDGFFGSEFYKMLLPPDAKKVESTLRNIGMGGQVDKAIL
                     AINRGAEDAVGFAKPIFVDAIKEMTLKDALNIIKGPKDGATNYFKDRTKEKLITAFTP
                     SVKSSLDKTDATKYYSDIITSYNKLPTTFKKINPDLTSYVVGRAVDALFDQVAKEEAN
                     IRANPLARTSDMLKKVFGQ"
     gene            complement(9125..>9653)
                     /locus_tag="PLO70_13370"
     CDS             complement(9125..>9653)
                     /locus_tag="PLO70_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQZ75504.1"
                     /translation="TDGFFGSQFYKVLLPPDAVKIENTLRDLGMGRMVDKAILSINRA
                     AEDAVGYAKPIFVSAIKDMTLMDAINIVKGPKDAATNYFKGKTREALLAAFLPSIKSS
                     LDKVDATKYYGDIVSTYNNFPTTFKKINPDLPGFVVEKATDALFDQIAKEEANIRENP
                     VARTTEILRKVFGGI"
BASE COUNT         2877 a         1969 c         2014 g         2693 t
ORIGIN      
        1 cctattttcc agacttccac ctatgtacag gaagcaccgg gagtaaacaa gggttttgaa
       61 tatgcccgca gccagaaccc tacccgcaaa gcgctggaag aagccctggc tattattgaa
      121 aatggaaagt ttggtttagc ctttagcagc ggtgttgcgg caacggatgc ggtgattaaa
      181 ttattagctc ccggcgatga ggtgatctgt gccaatgata tgtatggtgg tacgtatcgg
      241 ttatttacca aagtatttga aaaatttggt atcgtcttta catacgttga tactaccagt
      301 gtcagcaatg tgcaggctgc aattacggat aaaacaaaac tggtttggct ggaaacccca
      361 acaaacccat taatgaacat tacagatatt gcctctgttg cagctataac caaagcaaaa
      421 aaaatactgc ttgccgttga caacactttt gcttctcctt atctgcaaaa cccgcttgac
      481 ctgggtgcag atattgtcat gcattccgtt acaaaatatc ttggtggcca tagcgatgtg
      541 atacagggat gcctggtgat gaacgatgca gaactcagag agaaattata ttttatccag
      601 aaaagctgcg gtgccgtacc gggaccaatg gattgttttt tagtattaag aggaatcaaa
      661 acattaggtg tgcggatgaa agcccattgc gagaacgggg caaaaattgc aaactggctc
      721 cggagcaatc ctaaagtggg gaaagtgtac tggccgggat ttgaagatca tccgggccat
      781 gcggttgcca ggaaacaaat gcggggcttt ggtggtatga tttcttttga attaaaaaat
      841 gacagtgtgg aagaagccaa aagagtttta tcctccactc atttattttc actggcagaa
      901 agtctcggtg gtgtggaatc attgatcaat cacccggcca gtatgaccca tgcttccatt
      961 cccagggagg agagaataaa aaacggtctg tctgattcat taataaggct tagcgttggt
     1021 attgaagatg ctgatgacct gatccaggat ctgcaaaaag caattggttg atgaaaaaat
     1081 gaaagaacgt aatttaaaag agttcctcga taagaaggtt gatgattata atcaaccttc
     1141 tttcattaaa gatgatccta tttctgttcc gcatcttttc acaaaaaaac aggatataga
     1201 aattgcaggc ttttttgctg ccatctttgc ctggggcaac aggaccacca ttatcaataa
     1261 ggctaccgaa ctgatgcagt tgatggataa tgcgccatat gaattttgtc tgacccatga
     1321 cccgggcagc ctgaaaaaac taatgcgttt taagcaccgt acgttcaata caacagacct
     1381 gctttatttc attgaatttt tcaaattcca ttactgcagg cacaaaagcc ttgaaaccgc
     1441 ctttaccagg catggcagta caattgaaga aatgctgtcc ggatttcatc attatttctt
     1501 ttcgctggaa gaggtgcctg gcagaacaaa gaagcatatc gccacaccgg aaagaaaatc
     1561 aagctgcaaa aggctcaata tgtacctgag gtggatggtc cgaaaggata aaaaaggtgt
     1621 tgattttggc atttggaaaa atatctcccc gtctcagctg atctgtccgc tggacctgca
     1681 tgtggtcaga gtagcgaaac gattaaattt attacaacga aagcaaactg attggcaggc
     1741 agccgttgaa ttaacggaat atctccgaac tttggataag catgaccccg tgaaatatga
     1801 ttttgcttta tttggcatgg gtgtaatgga aaaatattaa ttacaatgac acaggttaat
     1861 acagcactgg ttcctgagat taatcaatca ctggagatac aattaccgct taccatttcc
     1921 ggggaagagt taagggaaaa actggcttca catgtcaatg agctcatcaa tcatgatttt
     1981 gaaaaattag tttttaacct ctaccgcatc gatgtaaatg aagccaggat gagggctttg
     2041 cttgcacaaa agggtggaga aaatgcagcc gcacttattg ctgaccttat cattgaacga
     2101 cagttacaaa aaataaaatc ccgccaacag tttcgtcagc gggacaatag tattaatgag
     2161 aatgaaaaat ggtagtgcta cttctttttc tcaaacatca gttcctttac tttctcttta
     2221 tcttcttttt cttttttcag gtcgaacata gtgccgaata tatgatccca taaggtagaa
     2281 cttacaccat acccattatg ttcattctta taatgatgca ggtgatgatt tctccaaagg
     2341 cctttcatcc atttgaacgg aggattccat gcatgaatag cataatgcat agtgccatac
     2401 atgaggtagc ctaataaaaa acccggaaag aacatgaaag tatacgtacc aatagcaaga
     2461 tacatcaggc caaatatggt agaagaaatg atcaggctgg gtaccggtgg cataaacagc
     2521 ctttgcttat ctcttggata gtggtgatga ttaccatgca gggtataaac aaattttgta
     2581 gccccgggtg ttttaggcac ccagtgaaaa atataacggt gggctatata ttcaaaaagt
     2641 gtccagaaaa acataccacc aaagaaaacg agcaaaatgg tgccggtgct gtaattatat
     2701 ttcgtggcag cgagataaat aatataagtg aggatcggga tatacattcc ccagatcacc
     2761 agcggatgtg ccttggtcag tgcctccagg aaatcattct tgaacaagcg ggcctgccct
     2821 ttattatgaa ttttttcgaa ttccatggcg caaagataag gaaggtcaat ggtgaaaaga
     2881 aagcgatcag gccgggtttt tataagacga gtgcctgatt ttaatcagcc tttaacagtt
     2941 catcaacacg ctgaaactga tagccttttg cttgcaggtg ttttatcaat tgcggcaaac
     3001 gtttataaaa tttatccgtt cgtttgggat cggttccgat gtgagataaa agaataaatc
     3061 cgttgagtcc gcccggtctt gcctgctcat atttaataat agactggtat atatcctcac
     3121 tgctccggta attcttatcg gccggcgtag tgtagtcggc atgactaagg gtaccgggag
     3181 taaaatttat cagctgaaag tccattttct tagtccatac cgcgatgctg tcattatacc
     3241 attcatacgg tggcaaaaag aagggagcat tttcttcccg gatcccaaaa cgactcatca
     3301 tactgtaatt tctctgcagg tctttggtga attgtgcttc tgttaccagt aaactgtccc
     3361 gtttacccca atcacaatac aataaatgtt tatctgaatg cggggcaatg tagtgcctgt
     3421 tatttttcag ctcaacgata atttttgtaa aagcaggatt acggaaaaaa tcaccggtaa
     3481 aaaagaagga agcttttatc ttttcctgtt gtaatgtgcg gacaataaaa tcaccaccgt
     3541 ctgcaaactc atgcccggta aaaacaaggg ctattttttt ctttgtacta tctcctctaa
     3601 tgatagcccc atgactatat tgtgagcctc ctgcaccgga ggctgctact tttttggctt
     3661 gcttcctgcc tccttcgcag caagcaaata gatcaacgat gccgttccat ccatagtagg
     3721 ctcgttggtg ctataatctc catagtcatc atgatatacg gcgaggtcac tttgaaattc
     3781 agcatattca tcgggctgat agagtgtaat accaataagc cctttgtata tgctggtgta
     3841 aaccggcccg tcaatcagtc caccatcaat cgggtagttt tttatatgtg taaatgcaga
     3901 atgcggatca actggagtat caccccatga gggcaaccca tacaccatgg atgtacccca
     3961 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcataa tgtccaagat
     4081 taataaaggg ataccattgg taatgtttag ctgtatctgc accaagccag ggggtgatcg
     4141 gttcagcatt tgcatagtcc atcaatgttt tcttaataat ttccttgtgg acaggcatgt
     4201 tatctttcga aagcacatat gcatttgcaa gttccatatc atcaatccaa ttgtcctcag
     4261 catatatata cggcgattta acggacgcag tctgcgtcac cccaggtact ttatttgcaa
     4321 aagcaaaagc agaaaaagtt ttttgtaaaa gcaggttact gtattcaata tttgtttttt
     4381 caaatgtttt tgatcctaat gcaaacgcac tggcaaactt ccccgccgta gaacttgtcc
     4441 ccgttgtatt attcataaac ctgccccgct gctgcggttc accacttaca aaataaaccg
     4501 gccgctcaaa ccctttgcca taaaaagtat cctgcccggg tatccgcatt cccatatggt
     4561 cccggtcatc tgctatctgg ttgaacatcc agtcgggcct tgggtgcatt ttcaataacc
     4621 agtccagtcc ccatttggct tcatccagta catcagccat tccatttttt ccatccaggc
     4681 cgtttgcttg tttctcatcg gtaaacactt taggaaaatc ccggtaagcc attagcaaat
     4741 gataagtagc attggcagat gttgcagagt attgtaaata atcagacgca tcatgccagc
     4801 cacccaccac atcaattctt gtgctatcct taataccagc tttttctcca tacaatacat
     4861 atccgtcgtg ggtgtgacag ctgtctttta aataaggatt gaaacccgta cgttgctgcc
     4921 gcatataccg caaacaaaaa tcagcggccc ctttataaac atcatctcct atttcaaatt
     4981 caggagattt tatattaccc gccagtaagt agtaacgacc tggtttctta aaatgagaaa
     5041 aattcagccg gtatgtttgt ttgaaaggtc catatgcccc aaaggctttt cccgccatcc
     5101 ccgttgccac tattttcttt gaaaccgaat taaccaattc ccatttaact atttcctttt
     5161 cttctttact gcaccaggta gcgactttca ctgcgccggg tgtatacccc agctggttga
     5221 tgcgtatcca gctttgcggt tgctgagcaa aggaggcaac aggtaagagt agtagtaata
     5281 aagaccattt tttcatcacc ccaatttaca aacaaccttg gtaacttagc agttcttaat
     5341 actacataga tgaaattagt tacctacctg aaagaagaaa gagaacaact gggcattctg
     5401 atcaatgata tggtatatga tatggaagtg cttcaccccg accttcccaa cagcatgaac
     5461 atgttattga attactggga agaatattac ccgatagcac aagctggtga attgttgctg
     5521 aaagaaggta ccaggacaag taataaagcg gtccctttga ctgaggtgca gttgctggca
     5581 cccgttccat tccccacctc ctgtcgtgat ggttatgcat tcagacaaca tgtggcggcg
     5641 gcgagaagaa atcgtaaagt ggagatgata ccggagtttg accaataccc gatcttctat
     5701 ttcaccaatc accacagtat tcagggccct ggtgatatcc gttgtatgcc tgaccatttt
     5761 gaaaaactgg attttgagct ggaggcggct atcgttattt gcaaacatgg cagaaatatc
     5821 agggcagaag aagccgatga atatatcggt ggtttgatga tcatgaatga tattagtgca
     5881 agacggttac aaatggaaga gatgttattg aacctcgggc cggcaaaggg aaaggatttt
     5941 tctactgcaa tcggtccatg gttagttacc ctggatgaac tggccccgtt tgaaatcccg
     6001 gccaaagaaa accataccgg caaaagctgg aacctgagaa tgcagtgccg ggtaaatggt
     6061 aagcaggtca gcgatggcaa tgtaggagat atggactgga catatgcaga gatcattgaa
     6121 agagcttcct acggtgttga cctgtatccc ggagatgtga ttggcagcgg cacagttgga
     6181 acagggtgtt tcctggaact gaacggaacg ggaaagctga atgatccgaa ttatcctgag
     6241 caatggttac aacccaatga tgtggtagaa atggaaatag atggcctggg taaactaagt
     6301 aatacgattg tggcggaaga taccgattgg agcattttga aattgaagaa atagaacact
     6361 gatcattatg attttcatga ttaccgcagt gaaatcataa cgtatcataa tcatcatcaa
     6421 aatctgcgtt cattttatga ttattgattt aaaagattta aaaccggctg aaaagcaata
     6481 ctacctgcaa catgttgttg caccaagacc tatttgtttt gcctccacaa taaataaagc
     6541 gggccatata aatctgagcc cctttagctt cttcaatctt ttttcttcta atcctcctat
     6601 tgtggtattc tcacctgcga ggagagtaag agataataca acaaagcata cactgcaaaa
     6661 tgtactggag gtgccggaag tggtcatcaa cattgtaacg tatgatatgg tacagcagac
     6721 ctcgctggcc agttgcgagt ttcccaaaga tgtcaatgaa tttaccaagg caggttttac
     6781 ccaggaaccc gcaaccatga taaagccccc gatggtcaaa gagagtaaag tgaaaatgga
     6841 atgtaaagtt gttgaagtaa aatcattggg aaatgaaggt ggtgcaggca acctggtcat
     6901 ttgcgaagta ataaggatgc atatagatga ttcccttttg gatgaaaata agaaactgga
     6961 tcagcgaaaa atcaaccatg tggcacggct tggcggggat tggtattgtg tggtgaatga
     7021 acacaacttg ttccaggtag aaaaacccaa tacacaattg ggtatcggta ttgatatgct
     7081 gccggcaacc atccgcaaca gtaaaatttt atccggaaat aatcttggcc agttagctaa
     7141 tgtgcatgag atgccggtga tagaaccgtc atttgatgat aatcacctga agcagatcat
     7201 tcaatactac agcgtcaacc cggatgagat ggaaaaagag ctgcattcct atgccaaaaa
     7261 attgctggaa gaagataaag taaaagaagc ctggcaagta ttgttagccg gctcctgatt
     7321 ttattaacgg tattccatct taaaaattca caaaacaaga ataagttttc tatctttaag
     7381 agagcctgtc acctgtgcat attttatgct taaagccttt ccttcatacc tgctgcttac
     7441 cctggtggtc attatcaccc tcttttcatg tgtaccccaa aaaggcgtta ccaatgcaaa
     7501 aaaagatctg gccactataa ataaccaact tcagcagcac agcactacat taaaagatct
     7561 ggaaaagcag cgaaagaaaa aagaacagca aaatgaaatt gatgatacgg ccaatacccg
     7621 gatcaaaaaa tttattgata aaacaaataa agaaatcgat acacttatta acagaaatac
     7681 gatcataatc ggcgaaacag tagtagaaaa atctgattgg gacaggctca gaaaaacatt
     7741 atcgttttcc cgtaagacct caaaaaatat tggtgacaga atattattac tgtccgatct
     7801 tatcaaccgg aatatggtgg taaggataga ccaggatgtg gtattcgagc caggcaagta
     7861 taatattacg cctgaagtag ctgctgcaat cgggaggctc tttgaaccgg cttctaaaga
     7921 aatagatctg tttgtaaaga aatatcccaa tttcccgctt tcactggtca taacagcaaa
     7981 aggatattca gatgctactt ctatcgccga gggctcatcc ctttacaagg aattaaaaga
     8041 caggttgaaa ttgcaaacag aaaaccccac gagtaaagag cttaataagg agctatcagt
     8101 agcccgggca gaagccgtta tcaacctgtt taaatcttac acattggaca ggggaagcaa
     8161 tgggggcagt gtaagcaata ttttatacct gcatgaagga aagggagaaa catctcctga
     8221 tccaaaagtc actgattata aaataaacga tgcaagaaga agagtggtgc tgctcttctg
     8281 gagtgttttc cctgaatgat tcttattgac caaacacttt cttcagcata tcagaagtcc
     8341 ttgccaaagg attagccctg atattagctt cttcttttgc cacctggtca aacaatgcat
     8401 ccaccgctct gccaaccaca tatgaagtca gatccgggtt aatttttttg aaagtggtcg
     8461 gcagtttatt atatgaagta attatatcgg aataatattt tgtggcatcc gttttatcca
     8521 aagagctttt tacagatggt gtaaaagcag tgatcaattt ttcttttgtt ctatccttaa
     8581 aataattggt agccccgtct tttggacctt taataatatt caaggcatct ttcagggtca
     8641 tttcttttat ggcatccaca aagatgggtt tggcaaaacc cactgcatct tcagcacccc
     8701 ggttgatggc caggatcgct ttatccacct gtccgcccat accaatattg cgtaaggtcg
     8761 attccacttt ctttgcatcg ggtggtaaca gcattttata aaattcgctg ccaaagaaac
     8821 catctgtctt attcaggtta agtactgccg tagtaacacc gtttgtcagt gcctctttaa
     8881 taccctgccc ggcttcattc tcggttatgc cagaagaaga ttttttgtta agtacatcct
     8941 ttagttttat ttgtgcaaag gaaccggcag gaagtacaat aaggctaaat gcaatgacga
     9001 taaaggaagt tttcatatct gtattgatta ttatattcat caaaaataac agcctttctg
     9061 cagtgcaaaa aggctgccag ctattttgac gttattttaa ggtatccgtt gcttcggggg
     9121 aatattaaat tccaccaaat actttacgca gaatctctgt ggttcttgct acggggtttt
     9181 ccctgatatt cgcttcttct ttggcgatct ggtcaaataa ggcatccgtt gctttttcta
     9241 ccacaaaacc aggtaaatcg ggattgatct ttttaaacgt ggtgggaaaa ttattgtagg
     9301 tactaacaat gtcgccataa tacttagtgg catccacttt atccagcgat gattttatcg
     9361 aaggaagaaa agcggccagt aaagcctccc tcgtttttcc tttgaaataa ttagtggcag
     9421 cgtctttggg tccttttaca atattgatgg catccatcag ggtcatatcc tttatagcgc
     9481 tgacaaaaat tggtttggca taacccactg catcctcagc agccctgttg atggagagaa
     9541 tggctttgtc caccatcctg cccataccca ggtctcttaa tgtattctcg atttttactg
     9601 catcaggtgg taataatact ttgtaaaact ggctgccgaa aaacccatct gtt
//
LOCUS       DAORTI010000125         9640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7539_length_9640_cov_5.476369, whole genome shotgun sequence.
ACCESSION   DAORTI010000125 DAORTI010000000
VERSION     DAORTI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7539_length_9640_cov_5.476369"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1117
                     /locus_tag="PLO70_13375"
     CDS             314..1117
                     /locus_tag="PLO70_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQZ75505.1"
                     /translation="MKKVLFFFLFASFFISCTKNLVTGRKQLDLVSETELQNLATQEY
                     QAFLNTNNVINSSNNRDAEMVRRVGTRISAAITSFYDKKGYTAVLDGYHWEFNLVDNK
                     EANAWCMPGGKVVVYTGLLPLTQNEAGLAIVMGHEIAHAVAQHGSERMSQALLQQLGG
                     VALQVAVANKPAETQNLFMTAYGIGSTVGGILPFSRKEESEADKYGLYFAAMGGYNPQ
                     EAIPFWERMSAAGGAKPPEFLSTHPSDQTRMEKIKRYMPQALKYYNPVK"
     gene            1287..1487
                     /locus_tag="PLO70_13380"
     CDS             1287..1487
                     /locus_tag="PLO70_13380"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQZ75506.1"
                     /translation="MTQDKTIVLHTYTDMLEAKMIQDKLKENGIDSFINDENVMGLDP
                     VGGAELRIFEKDKVAAEKIISA"
     gene            1613..2329
                     /locus_tag="PLO70_13385"
     CDS             1613..2329
                     /locus_tag="PLO70_13385"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ75507.1"
                     /translation="MKNSLKDKVAYITGGSKGIGYGVAKALLAQGMKVAITSRNLEAA
                     KEAAASLSADPAKVLALKSNVSSLTDETNAIAATIGQFGQLDVLIANAGVGHFDSIEN
                     LSEAEWKETIDTNLTGVFHSVKASLPALKQSRGYIITIASLAGANFFENGAAYNASKF
                     GLVGFTQAIMLDLRKHGIKVTTIMPGSVATQFGNHIPSVADAWKIQPEDIGQMVTDLL
                     QMNVRTLPSKIEVRPSIPVK"
     gene            complement(2467..3324)
                     /locus_tag="PLO70_13390"
     CDS             complement(2467..3324)
                     /locus_tag="PLO70_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75508.1"
                     /translation="MTRKYMPALTGAGIKLFKSVCITSYLFCATVLNAQEHYTINWDS
                     APLNPVPFAYRSNHLNLKGKVKTLLEINQWKDTITRRFDAAGFIKSVSTAGPENMFPG
                     TTTYTYKFPEGYLTTVWKTDMEEIVYRYKFNKQKQIIESGILNNLKIENPVKYGYDPS
                     GRLAYDSSALTKIYTYNNEGQLTGLQENGLFGVKINSSYTYKKENENLLISILVKRVD
                     GSYTTTTETTETYNPQRELINVAGKKEKKVYYYQFDTVGNWVKRTEVTFSNNAQTNTV
                     ETKRIIEYY"
     gene            complement(3596..4351)
                     /locus_tag="PLO70_13395"
     CDS             complement(3596..4351)
                     /locus_tag="PLO70_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ75509.1"
                     /translation="MNNSLTVDINRNYVEAGCGEPIILLYGLFGSVKNFEPLINHLKQ
                     SYRVIVPVFPFFEKGMSISIFSLTEFLDELTKELSLDQFHLLGNSMGGHIALLYALNH
                     PEKITSLILSGSSGLYESGMGDTFPKRSDYNYIKTKTELTFYDPKIASKELVDEIFAV
                     VNSRKVIQILSLAKSTIHHNLEKELLNIKTDCCLIWGKNDKVTPPEVAIKFKKLIPHS
                     KLYWIDQCGHVPMLETPAAFNTVLDEFLLSLRK"
     gene            4636..5136
                     /locus_tag="PLO70_13400"
     CDS             4636..5136
                     /locus_tag="PLO70_13400"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQZ75510.1"
                     /translation="MSIGKRFWAELQQEAANTRRLLAVVPFDKADFKPHEKSMSLKRL
                     ASHVAEINGWWKECLVHDELDFAKDAGKPKEYHSTEDIVAWHDELVAKAEIILNDTPD
                     EEFAKPWTMRQGEMIFFTLPKAEVVRTWCLNHLYHHRGQLTVYLRLLNVPLPGMYGPT
                     ADDQGM"
     gene            5427..5717
                     /locus_tag="PLO70_13405"
     CDS             5427..5717
                     /locus_tag="PLO70_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75511.1"
                     /translation="MDILPLLIQLASGAVGGNVAGKLIPKLSLGNIGNSIVGILGGGL
                     GGQLLGMLGLGGVAEGGGMDINSIISSVAGGGVGGGVLMAIVGFVKGLMNKK"
     gene            5942..7387
                     /locus_tag="PLO70_13410"
     CDS             5942..7387
                     /locus_tag="PLO70_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQZ75512.1"
                     /translation="MQVLKSFLTRLIQFFLFIHLAVSALAQKSILYFSNDTAGFASAK
                     IPKAFLEKDMQYWQTVMEESHVNPYHAISRDALLKLQQEILQTLPDSISHFQASFAIS
                     RLIGSLDEGHLGFASNAVIDSLFLNHCIRFPYLLQDIDNGSFLVQRDLSPENKLPAFS
                     RIISINDIPVNQLYNRYSTFYGGLEPWKKLMVKNAIRKLLYMDGITSPFRIKAVVNND
                     TIVFSATGYSKQQADSITKVILSEMKPPLPFSLQFLPGNIALIEFNSMKGSLRDSFAV
                     FLRRSFSEIRLRRAAGVIIDLRKNGGGDSGLGDTLISYISSKPYRNAGGMKMRISKHS
                     KAWAAMHGATDPFKDWENGKLYEYKATTLTTPAANPLRYNGKVAVLIGTGTFSSANML
                     TNAIKDFQLATLVGESTAEPANDYGEIFSFMLPYTHIVAQGAFKMFTRANGDEKDFNG
                     IRPDIEVTISAKDISAKQDTVLDRAIAWLLQ"
     gene            7523..7978
                     /locus_tag="PLO70_13415"
     CDS             7523..7978
                     /locus_tag="PLO70_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75513.1"
                     /translation="MQTIHQKSYCRISFFLLLSLISFATTTLAQSKDVKKILAVLRLE
                     EEFWSKGDIEGYVSLYAPLDSTRMILSKGAAYGKKNILAFYKKYWPREKMGTLVLDGE
                     SLERLSRKFYYVAGYFHVRYPDGKTIDGRYSSLMIKIKGKWYLYTDHSG"
     gene            complement(8020..9255)
                     /locus_tag="PLO70_13420"
     CDS             complement(8020..9255)
                     /locus_tag="PLO70_13420"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQZ75514.1"
                     /translation="MKSKFIFSIPAMLISIYSCGNAGNNAASTEQMKDTTKFPPVETK
                     KPNADYKPAFKGQTRIAGVKTVTPYKVEKIAEKLGRPWAVIPLSDGRLLITEKSGYMQ
                     IHDANGKLIKKITGFPAVDAGGQGGMLDVALDPGYSKNKTIYWSYSEKYGNGNLMAVA
                     KGQLNEAAAQIENPIVIFRATPALNSSLHFGSRLVFDKDGNLFVSTGERSKPEGRKQA
                     QWLNSGLGKIFKITKEGKPAPGNPFITRKDAMPEIFAYGIRNAQSLDIHPVTGELWEA
                     EFGPRGGDELNIIKAGKNYGWPIITYGIEYSGPKVGDGIQQQSGMEQPVYYWDPVLSP
                     GGMVFYRGNSIPEWKNNLFICGLSSAHIARLVIVNNKVVGEERLLEDKYERFRDIAYL
                     DGMLYAVTDTGNMYRISKK"
BASE COUNT         2785 a         1817 c         2024 g         3014 t
ORIGIN      
        1 gaatatgtaa aatgcagcat ttaggtatgc attgccggaa ccttggcttg ttgatatacc
       61 aaacctttgc tgattcaatt aatttcagag tttgccagct tcctctttat agcatatacc
      121 agacctttac cgatgaagaa aagctggtaa gttcagtacg cttgcaagcg gatgcaatca
      181 tcccggaaaa tcaaatccct gccctgagca aaatcattgg gttatctact ctcggtcatt
      241 tttatgggta gtgttgttat gctaccttaa caacagcaat gaatattttt tgcataactg
      301 taatttatta ataatgaaaa aggtattatt cttctttttg tttgcttctt ttttcatcag
      361 ctgcacaaaa aatcttgtaa caggccgcaa gcaattagac ctggtatcag agacggaatt
      421 acaaaactta gccacccagg agtaccaggc ctttttgaat actaataatg tgataaattc
      481 cagtaacaac agggatgccg aaatggtacg ccgtgttgga acccgtattt cagctgcaat
      541 tacttcattt tacgataaaa aaggatatac tgcagtattg gatggttacc attgggaatt
      601 taacctggta gataacaaag aagccaatgc atggtgtatg cccgggggta aagttgttgt
      661 ttacaccggt ttgttgccct taacccaaaa cgaagcaggt ttagccattg taatgggcca
      721 cgaaatagcc catgctgttg cacagcatgg tagtgaaagg atgagccagg ccttgttaca
      781 acaattaggt ggagttgctt tacaggtagc tgttgctaat aaacctgctg aaacacagaa
      841 tctttttatg actgcttatg gtattggcag caccgtaggt ggtatcttgc cattctcccg
      901 taaagaagag tcggaagcag ataaatacgg gctatatttt gctgctatgg gaggttacaa
      961 tccacaggag gccattccct tctgggaaag aatgtcagca gccgggggcg caaagccacc
     1021 tgagttttta agtacccacc cgtctgatca aacaagaatg gaaaaaatta aacgatatat
     1081 gccccaggcg ttaaaatatt ataaccctgt aaaatgatag atgttgatat tgcctgttac
     1141 aatcctggtg atcgtcagcg tcaacactgg gaaatagctg aaatgcagtt gacattatgt
     1201 tcaattattt ttttgcatat tttttgccct tgaaaacagt taacaagcag tattagtaaa
     1261 tatttattca aaataaatta ttcgtaatga cacaagataa gacaatcgtt ctgcacactt
     1321 atactgatat gctggaagca aaaatgatcc aggataaatt gaaagaaaac ggtattgatt
     1381 cttttattaa tgatgaaaat gtaatgggtt tagatccggt aggaggagca gagctaagaa
     1441 tatttgaaaa agacaaagta gctgctgaaa agattatcag tgcttgattg tccaaggtca
     1501 atctatggta gtttttgatg ttatcactcc tggctggcag gagcggcagg attattctct
     1561 tctgctgatt tgaaaggaag tgattatttt actgtacgaa tatcttacaa taatgaagaa
     1621 tagtttaaaa gacaaagtag cctacatcac tggcggcagt aaaggcattg gttatggagt
     1681 tgccaaagca ttgttggctc agggcatgaa agtggctata accagccgga acctggaagc
     1741 tgcaaaagag gctgcggcct cattgagtgc tgaccctgcg aaagtactgg ccctgaaatc
     1801 caatgtgagt tcccttaccg atgaaactaa tgctatagcg gcaaccatcg gtcaattcgg
     1861 gcaattggat gtgttgattg ccaatgccgg ggtaggtcat tttgattcta ttgaaaattt
     1921 atctgaagcg gaatggaagg aaaccattga tacaaacctg acaggtgttt tccatagcgt
     1981 aaaagccagc cttcctgctt taaagcaatc aaggggttat attatcacta ttgccagcct
     2041 ggccggagca aatttttttg aaaacggggc tgcttataat gccagtaagt tcggacttgt
     2101 tgggtttacc caggcgatca tgcttgatct gcgtaagcat gggataaaag tgacgactat
     2161 tatgcccggt tccgtggcca ctcagtttgg taatcatatt ccttctgtgg ccgatgcctg
     2221 gaagatacaa ccggaagata tcggccagat ggtgacggac ctgttacaaa tgaatgtaag
     2281 gaccttgccc agtaagatag aagtaaggcc ttctataccg gttaagtaaa ggctgctatc
     2341 aatgaattat tataacaggc atcaataatc agtatccggg attttttaat ctgttaccag
     2401 ttgctgtttt gaggagggtt atataccggg ccacaccact tgtaacccgg gtatttttgg
     2461 ctataactaa taatattcga ttattcgttt agtttccaca gtgtttgttt gtgcattatt
     2521 ggaaaaggtc acttctgttc ttttgaccca gtttcccacg gtgtcaaact ggtaatagta
     2581 cactttcttt tcttttttgc ccgctacatt tatcaattct ctttgtggat tataggtttc
     2641 tgtagtttca gtagttgtcg tatagcttcc atccactctc ttaacgagaa tactgatgag
     2701 caggttctcg ttttcttttt tgtaggtata gctgctattg atctttacac caaacaggcc
     2761 attctcttgc aagccggtca attgtccttc attattgtag gtatagattt tggttaaggc
     2821 tgatgagtca taggcaagtc ttccggatgg gtcgtaccca tattttaccg ggttttcaat
     2881 tttaagattg ttaaggattc ctgattcaat tatttgtttc tgcttattga atttataacg
     2941 gtaaacgatc tcttccatat cagttttcca cacagtggta agatatccct cggggaattt
     3001 atatgtataa gtagtggtgc cgggaaacat gttttctggg ccggctgtgg atactgattt
     3061 tataaatcct gctgcatcaa atcttctggt aattgtatct ttccattggt tgatctcgag
     3121 cagggtcttt acttttcctt tcaggtttaa atggttgctt ctataggcaa acggaaccgg
     3181 gtttaacgga gctgaatccc aattgatagt ataatgttcc tgagcattca gcactgttgc
     3241 acaaaataga tatgaagtga tacaaacact tttaaagagt ttgataccgg caccggtcaa
     3301 agctggcatg tatttacgag tcataaaata tattttgctg agtttcacta ttgtttttta
     3361 ctttaattat ttacttgaat aaatataaaa aaatggaata actgcttaat accctgtatt
     3421 gggtataaaa aagctcttcg attaagatct aatccaataa tatgtattag tatattattg
     3481 acctgaagct tcaggaatga attttcaggg taagggcaag gactattaat agcgcctccc
     3541 cgtcggtacc aatttcataa tctaggatta caaaataaag taaaacgagc tgtaattatt
     3601 ttcgcagcga aaggagaaat tcgtccagga cggtattaaa tgcggcaggt gtttccagca
     3661 ttggcacatg tccgcattga tcgatccagt acaattttga atgtgggatc agtttcttga
     3721 actttatagc cacttcagga ggagttacct tgtcattttt tccccatatc aggcagcaat
     3781 ctgtttttat gttcagtagt tctttttcca ggttatggtg gatagtggat ttggccagcg
     3841 ataatatctg gatgaccttc cggctgttaa ctacagcaaa tatttcatcc actaattctt
     3901 tggaggcaat tttcggatcg taaaaagtaa gttctgtttt tgtttttata tagttgtaat
     3961 cacttctttt gggaaacgta tcacccattc cactttcata caagccggaa cttccgctga
     4021 gaattaaaga agtaattttt tcaggatgat tcaatgcgta taagagggca atatggccgc
     4081 ccatggaatt gcccagtaaa tggaattggt ccagcgataa ttctttagtt agctcatcca
     4141 ggaattcagt gagtgaaaaa atactgatgg acattccttt ttcaaaaaag gggaaaaccg
     4201 gaacaatgac ccggtaagat tgtttcaggt ggttaatgag aggttcaaag tttttaacag
     4261 acccaaataa accatataaa agaatgatgg gttcaccgca acctgcctct acgtagtttc
     4321 tattgatatc tactgtcaat gaattgttca taaattccca tttacatacc cagcttagca
     4381 gaagatcggt tatttaatgt atactttcta tcactaaatt aaatttgccc gattcctttt
     4441 cagcattgaa acttaaaggt tgaaaagtaa taaatttatg taataaacat aatgacttga
     4501 ccacatactt ctctttttca aacctgtagc cgttgtaaga agtctgattt tgtttgtacc
     4561 tgaggatata tggaatagtc atgattacag tgctgaaaga atacatactt ttacgtaccg
     4621 aaaaaaaata atattatgtc tatcggaaaa agattctggg cagaattgca gcaggaagct
     4681 gccaataccc gcaggttatt agctgttgtg ccttttgata aggcagattt taagccacat
     4741 gaaaagtcca tgtcgctaaa acgactggcg agccatgtag ctgagataaa tggttggtgg
     4801 aaggagtgcc tggttcatga tgaactggat tttgcaaaag atgcgggtaa accaaaagaa
     4861 tatcattcaa cagaagatat agttgcctgg catgatgaac tggtggcaaa agcggaaata
     4921 attttaaatg atacgccaga tgaagagttt gccaaaccct ggacaatgcg gcagggtgaa
     4981 atgatatttt tcactttacc caaagcagaa gtagtacgca cctggtgcct gaatcatctt
     5041 tatcatcacc gcgggcagtt gaccgtttat ctccggttat taaatgttcc actgccggga
     5101 atgtatgggc cgacagcaga tgaccagggg atgtaactga tatttttagt gaatctattt
     5161 catattagtt tctatagcct ggcttataag aggatctttc aaaaaaattt tgaattgctt
     5221 actccactac ttccagccac tgagtaagga attttttgtt ccattctagc attatgtatc
     5281 agtttaataa accggaaaac catggttttt gagtagccct ttaaagccgt gccgtttatc
     5341 aggtagtgac tggaataaaa ttaatttcaa atacactgga taaaatattg tatcttctgt
     5401 ttcgtattta accaaaacaa tttattatgg atatacttcc tttactcatt cagttagctt
     5461 ccggtgcagt cggaggtaat gttgcgggta aattaatacc taaattatca ttgggaaata
     5521 ttggtaattc tattgttggt atccttggtg gagggcttgg cgggcaattg ctgggcatgc
     5581 tcggtttggg tggcgttgca gagggtggtg gtatggatat taacagtatc atcagcagtg
     5641 tagccggtgg tggtgttgga ggtggggtgc tgatggctat tgtgggtttt gtaaaagggt
     5701 taatgaataa gaagtgaaat tctcttttca ctatggcaga ggttcggtaa aaagggattt
     5761 gctgtgtttt ttgttttgat ggcatggctt ctggaagtct cctgcaacaa cttcagcaac
     5821 taaatactat tttacgctat taaagaatac ccggattcat tagtaatata tttccattct
     5881 tcaccacctg tatttaatta ttttattaat atttgcagta gcaataaatg ccagcttcac
     5941 tatgcaggta ctaaaatctt ttctgaccag gctcatccag tttttcttgt tcatccatct
     6001 tgcggtttct gccctggccc aaaaaagcat tttatatttt tccaatgata cggcaggatt
     6061 tgccagtgcc aaaattccca aagcattcct ggaaaaagat atgcagtact ggcaaacggt
     6121 aatggaagaa agtcatgtaa atccttatca tgctatcagc agggatgccc ttttaaaatt
     6181 acaacaggaa atattgcaaa cacttccgga cagtatcagt catttccagg cgagttttgc
     6241 cattagccgt ctaattggat ccctggacga agggcattta ggttttgcca gcaatgctgt
     6301 tattgatagt ttattcctca atcattgtat tcggttccct tacttacttc aggatataga
     6361 caacgggagt ttcctggtac aaagggatct gagcccggaa aataagctgc cggcgttcag
     6421 tcgtatcatt tcaatcaatg atatcccggt aaatcaatta tataaccgtt attcaacatt
     6481 ctacggcgga ctggaaccct ggaaaaagct gatggtaaaa aatgctatcc gtaagctgct
     6541 ttatatggat gggattacta gtccgtttag aattaaagcc gttgtaaata acgataccat
     6601 tgttttcagt gccaccgggt acagcaaaca acaggctgat agtataacca aagtaatact
     6661 atcggaaatg aaaccaccgt tgcccttttc actgcagttc cttcccggta atattgcgct
     6721 gattgaattc aatagtatga agggatctct ccgggattca tttgcagttt ttctccggcg
     6781 ctccttttcg gagatcaggc tccgccgggc tgcaggtgtt attattgacc tgcgaaaaaa
     6841 tggtggtggc gacagtggtc ttggagatac attgatcagc tatattagtt ccaaacccta
     6901 tcgcaatgcc ggtggcatga agatgcgtat cagtaagcac agcaaagcct gggctgcgat
     6961 gcatggagct acagatcctt tcaaagactg ggaaaacggt aaattatacg aatacaaggc
     7021 cacaacatta actacaccgg cggctaatcc gcttcgctat aatggtaagg tagcggtact
     7081 aattggcacc ggaactttca gcagtgccaa tatgctgacc aatgcgatta aagattttca
     7141 attggccacc ctggtcggcg aaagtacagc tgaacctgcc aatgattatg gtgaaatatt
     7201 ttctttcatg ttgccatata ctcatattgt ggcgcaaggg gcatttaaaa tgttcaccag
     7261 ggccaatggc gatgaaaaag atttcaatgg tatcaggccg gatattgaag ttactatttc
     7321 agcaaaagat atcagtgcaa agcaggatac tgtattggat agggccatag catggctgct
     7381 gcaatgaaat atcttctcta aattatcatt agaaatattg ctgatagttt gttaatcaga
     7441 tccggactaa gaacaggaat tgatgagcgg taactttacc tattgctttc ttggataatt
     7501 tcagcataca aaaatcagcc gtatgcaaac aattcatcaa aaatcttatt gcaggatatc
     7561 cttcttttta ttgttaagcc ttatcagttt tgcaacaaca accttagctc aatcaaaaga
     7621 tgttaaaaag atactcgctg ttttaagatt ggaggaagaa ttctggagta aaggagatat
     7681 tgagggttat gtaagcctgt atgccccatt ggattccacc cgtatgatct taagtaaagg
     7741 tgccgcctat ggtaaaaaaa atatcctggc attttataaa aaatactggc cgagggaaaa
     7801 aatgggaaca ctggtactgg atggtgaaag cctggaaagg ttatcccgca aattctatta
     7861 cgttgccggt tattttcatg tgcgctaccc cgacgggaaa accatcgatg gccgttattc
     7921 cagcctcatg attaagataa aagggaagtg gtatttatat acagaccatt ccggttagta
     7981 ataaacaatc agtcaacaat aaacggaaaa tggacctgat tattttttac tgatcctgta
     8041 catattacct gtatctgtaa cagcatagag cataccatct aaataagcaa tatccctgaa
     8101 tctttcatat ttgtcttcca gcagtctctc ttcacccacc actttattat ttacaataac
     8161 cagtcgtgca atatgagcac tgctgagtcc acagataaac aggttgtttt tccattccgg
     8221 tatactgttg cccctataaa ataccattcc tccgggtgat agcaccggat cccagtagta
     8281 taccggttgt tccatcccgg attgctgttg gataccatca cctactttag gaccggaata
     8341 ttcaatgcca taggtgatta ttggccatcc ataatttttg ccagctttaa taatattgag
     8401 ttcatcacca ccccttggac cgaattccgc ttcccacagt tcgccggtaa ccggatgtat
     8461 atcgaggctt tgcgcattgc gtatcccgta agcaaagatc tcaggcatcg catcttttcg
     8521 ggtaatgaat ggattgcccg gtgctggttt accttctttt gtgatcttaa aaattttacc
     8581 caaaccggag tttaaccatt gcgcctgttt ccttccttcc ggttttgatc tttcaccggt
     8641 gctcacaaaa agatttccgt ctttatcaaa caccagcctg gaaccaaaat gcagactgct
     8701 gttcagggca ggggtagccc ggaaaataac tatggggttt tcgatttgtg ctgctgcttc
     8761 atttagctgt cctttggcta ctgccatcag gttaccattt ccgtattttt cagaataaga
     8821 ccaatagatg gttttgtttt ttgaataacc gggatccagt gccacatcaa gcataccgcc
     8881 ttgtcctccg gcatccactg cggggaatcc ggttattttt ttaatcaatt taccattggc
     8941 atcatgaatt tgcatatacc ctgatttttc agtaataagc aaacgtccat ctgaaagagg
     9001 gataacggcc cagggccttc ctaatttctc tgctatcttt tccaccttat agggtgttac
     9061 tgtcttaaca ccggctatcc ttgtttgtcc tttaaaggca ggtttgtaat cggcattggg
     9121 ctttttagtt tccacggggg gaaacttagt tgtgtctttc atctgttcag ttgaggctgc
     9181 attattccct gcattaccac aggaataaat ggagatcagc attgcaggga tactgaatat
     9241 gaattttgac ttcatggttc ttttttagtt aggaatcgta aaaataggaa actgttacaa
     9301 actggtttgt tatttatagt tgggtatcaa attttacatt ttactgatat aactgatttt
     9361 gcaggcagtt gttcagtatt atcttaaata atgtttgatg tgaaattgca atttgcaaat
     9421 aagggagttt atttcaccca attcaattaa caaaaaaatt tgaagctttt agcggaaata
     9481 aactggtatt aagattcctc tgagtggccc gaaaagttat aattcaatca ccataccttc
     9541 ttctgccagg tcataatgaa aagaataatt atttctcatt ttgagttcag tcagtagctc
     9601 cccgagaaat gtatcggtat gattagggtc atgatgcgcc
//
LOCUS       DAORTI010000126         9503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7715_length_9503_cov_6.605631, whole genome shotgun sequence.
ACCESSION   DAORTI010000126 DAORTI010000000
VERSION     DAORTI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7715_length_9503_cov_6.605631"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..692
                     /locus_tag="PLO70_13425"
     CDS             126..692
                     /locus_tag="PLO70_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HQZ75515.1"
                     /translation="MTNFIKPYNPDWITEFEALKNYLITGLKDFDIDIQHIGSTSIPG
                     LCAKPIIDIDIILKQKIQLGDISAKLEKLGYKNKGEQGISGRHAFRQTSGLTPSTADR
                     KKWQEHHLYVCYPESLALKNHLLFRDVLIQNKDLVNEYAQLKINLTKEKGMTREKYTM
                     QKTHFILSVLALNGLDEKELNEIKAANL"
     gene            875..2014
                     /locus_tag="PLO70_13430"
     CDS             875..2014
                     /locus_tag="PLO70_13430"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ75516.1"
                     /translation="MITKPSILAPLRNYTFILLSVSFASCNTDNKTGNSNNTATESTS
                     VPGNEFNTDNLRTITTTAINKNIRSIFQDKKGNYWFGTNGAGVYRYDGKTIRHFTAKD
                     GLAGNQVQSIQEDQTGNIFFGTGGFGISKFNGQSFTTLTTKENVQVSNGALRDWKATP
                     TDLWFYAGGGAFRYNGNSLVYLPLDNPGLNPKNPQSSPYELSSYAVYSILQDKKGNIW
                     FGTQARGVCRFDGQSFTWFTEKGLGGPAVLGLFEDSHGNIWFGNNGAGLFRYDGKTLT
                     NFTAEKKLGNNEFSLAGNPGPGTLARIYSINEDKTGNLWIGTLDAGAWRYDGNTLTNF
                     TTKDGLTSNAINTIYKDKFGELWFGTDGDGMCRFNGKTFTALVVQ"
     gene            2057..2911
                     /locus_tag="PLO70_13435"
     CDS             2057..2911
                     /locus_tag="PLO70_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HQZ75517.1"
                     /translation="MKTSKHVLIALLQFTIISTKAQVGIGTPAPAASAQLDISSTSKG
                     LLVPRMTQAQRTTIATPDAGLLVYQTDGASGFYYFKSGGWLRLVAESSYPAVTICTQL
                     WMDKNLDVTTYRNGDIIPYVTDQTEWAALTTGAWCYYNNDPSSNAVYGKLYNWYAIND
                     SRGLAPAGWHVATDAEWSTLSTCLGGASVAGGKMKVTGTTTWASPNNNASNSSGFAAL
                     PGGIRTNGSSNYFVDLNYFSHFWSATELIPTPQLAFSRYIINSGGDLTQYNGFAKSFG
                     LSVRCIRD"
     gene            3136..4386
                     /locus_tag="PLO70_13440"
     CDS             3136..4386
                     /locus_tag="PLO70_13440"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQZ75518.1"
                     /translation="MKLQSLIIALSLLTTATAQNVGIGTTTPVARLHVADSNVLFTGP
                     LTVPGTTTYDPPASGAGSRMMWYPQKAAFRVGFAESTHWDKDNIGRFSFSSGYNGKAI
                     GFASTSMGVTTIASGFASTSMGSFTTANGDYSTSMGKNTTANGIYSTSMGYYTSANGD
                     YSTSMGNFTIATSENSLVIGKYNENTNTNRLFEIGNGTGVNARSNALTVLVDGNIGIG
                     TTTPTRPLSFPPFLGEKILLYPGGIGEVGIGVYGNELRLHADNPGAKVSFGTQTNAGA
                     FTEAGKFEISGGYALSVFGSIWANGTTYASDERFKQNITAIKSPLQKLLQLNGVEYEM
                     KTDEFSKNHFLPGRQMGLLAQNVETIVPEAVHEKDGYKGVDYARLVPLLIESIKEQNK
                     KIEEQQKQIDEQRKMIEELLKKQL"
     gene            4383..5843
                     /locus_tag="PLO70_13445"
     CDS             4383..5843
                     /locus_tag="PLO70_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HQZ75519.1"
                     /translation="MKKMFSLVIVVSSCMHVEAQNVGIGTTTPLARLHVTDSNVVFSA
                     TGLAVPVPGNPPVSGTGRRMMWYADKGAFRVGYAAGTSWDRTNVGNYSFASGYGSVAS
                     GDYSSALGLNNKAAGMSSVALGNNAQANTDYSTAIGGVSFANGLSSTAIGFQAFATGD
                     YSTVLGYNNISKALGGIVMGMYNDATDTPNPNDTSSLDRIFQIGNGYYDAIIDDEVRR
                     NAITVLRNGNTGIGTSTPAASAVLDVSSTTKGFLPPRMTAAQRTAIATPAEGLLVYQT
                     DAVAGFYYIRNGIWTGLNDAATFPANTICTQQWMDKNLDVNTYRNGDPIPYVTDPVAW
                     AALTTGAWCYYNNDPSNNATYGKLYNWYAVNDPRGLAPAGWHVPSTTEWTTLETCLGG
                     PTVAGGKMKVNGTTTWQSPNTGASNSSGFAGHPGGYRDELNGSYGFIGQYGNWWSSTQ
                     SGPSNAFFRYLYYADASFYVFSLPKQRGQFVRCIKD"
     gene            6220..6933
                     /locus_tag="PLO70_13450"
     CDS             6220..6933
                     /locus_tag="PLO70_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75520.1"
                     /translation="MRIVTWNCNGALRNKFEHLSELNADLYIIQECENPESAKHQQYI
                     SWAGNHLWKGDNKNKGLGVFAKNKTNLELIDWCNIYKNHEVKHFLPCLVNSDFQLLSI
                     WTSQNKSPNFGYIGQLWKYLEINKSKFKKIILAGDFNSNTIWDEWDRWWNHSDVVSDL
                     SKIEINSLYHRYFNEEQGKETKPTFFLQRNRAKPYHIDYIFASKEIENNMTNFEIATI
                     DKWLNYSDHLPVICEINTL"
     gene            7033..7485
                     /locus_tag="PLO70_13455"
     CDS             7033..7485
                     /locus_tag="PLO70_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ75521.1"
                     /translation="MLTCIRTNSSQNHFQQLVRELDADLSVRDGEEHAFYAQFNKTDR
                     IKHVVVAYDNETPVGCGAIKEYAADTMEVKRMYVPLHRRGQGIASLILEELEKWCREL
                     NYNKCILETGIRQPEAIRLYEKNGYARIPNYGQYANMDNSVCFEKKLG"
     gene            7627..8886
                     /locus_tag="PLO70_13460"
     CDS             7627..8886
                     /locus_tag="PLO70_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ75522.1"
                     /translation="MADIFERLLKNAGPLGQHRERAHGYFAFPKLEGEISSRMKFRGK
                     DMIVWSLNNYLGLANHPEVRKADAEGAAQYGLAYPMGARMMSGNSNLHEQLEAELAAF
                     EHKEDAALLNYGYQGMVSIIDVLCSRHDVIVYDAESHACIIDGVRLHPGHRFVFKHNE
                     VEDCEKQLQRAAALIEKQKAGGILVITEGVFGMAGDQGKLKEIAALKEKYQFRLLVDD
                     AHGFGTLGKTGAGAGEEQGVQDKIDLYFSTFAKSMASIGAFVAGDKKIIDFIRYNIRS
                     QIFAKSLPMPLVIGNLKRLELLRNHPELKEKLWSNARKLQNGLKERGFDIGNTDSVVT
                     PVYMKGDVPEATAMVMDLRENYGIFASIVVYPVIPKGHIIYRLIPSAIHNDEDIELTL
                     KAFSETKKKLDAGEYKVEAIPDMAETK"
     gene            9137..>9503
                     /locus_tag="PLO70_13465"
     CDS             9137..>9503
                     /locus_tag="PLO70_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75523.1"
                     /translation="MRKLFLLLFLLPVMAVAQKKSITLEDIYKKGTFRGEPVRAVFDQ
                     TTKEPELKLEGLKDESGKTFGQPEDVITSAANPVVAILKKGTEPIYRRSSKSYIYIYD
                     TTTKKLVKPENEKVLHPTFS"
BASE COUNT         3036 a         1895 c         2020 g         2552 t
ORIGIN      
        1 tacacacaac ctgaacggtg taaatttacc aggcagcagt tacgggctga tttttttcaa
       61 ataaaggcac atccaggccc tgaccgttag cggtaataca atcggtcact atttccaatt
      121 gctatatgac aaattttata aagccatata acccagactg gataacagaa tttgaagcgc
      181 taaaaaacta ccttataaca ggtttaaaag attttgacat tgatatacaa catataggaa
      241 gtacatctat acctggactc tgtgcaaagc ccattattga cattgacatc atcttaaaac
      301 aaaaaataca acttggtgac atttctgcga aacttgaaaa acttggatat aaaaacaaag
      361 gcgaacaagg aatttccgga cgccacgcct ttagacaaac ttccggcctg acaccctcaa
      421 ctgctgaccg aaaaaaatgg caagaacatc atctttatgt ttgctacccg gaaagtttag
      481 ctttaaaaaa tcatttactt ttcagagacg tattaataca aaacaaggac cttgtaaatg
      541 aatatgctca attaaaaata aatctgacaa aagaaaaagg tatgactaga gagaaatata
      601 caatgcaaaa aactcacttt attttatcag tacttgcact taacgggctt gacgaaaaag
      661 agttaaatga aataaaagct gcaaacctgt aaatgtacta tcgctaaaaa ggactgtgta
      721 aaagccaatc aatttcttct tttttacatt ttgcctgtgt aatgaaccgg gcagatggaa
      781 cccggaatat cactacattg catagctgtg tctataagta accattctgc agtaacgagt
      841 aaacaatgaa caggaaggca agtaaattag aaaaatgatc actaaaccat ccatccttgc
      901 accattaaga aattatactt ttatattact atccgtttca tttgcttcct gcaacacgga
      961 taacaaaaca ggcaacagca acaatactgc aacagaatcc acttcagttc ccggcaacga
     1021 attcaatact gataatctac gtacgataac gacaactgcc attaacaaaa acatccgcag
     1081 tattttccag gataaaaaag gcaattactg gttcggcacc aatggtgctg gtgtgtatcg
     1141 ctatgacgga aaaacgatac ggcattttac tgcgaaagat ggcctggcag gcaaccaggt
     1201 tcaaagtata caagaagatc aaaccggtaa tatttttttc ggaacaggtg gattcggcat
     1261 cagtaagttt aacggacaaa gttttaccac gctgacaaca aaagaaaatg tacaagtaag
     1321 caacggggct ctccgggact ggaaagcaac acccaccgac ctatggttct atgctggtgg
     1381 tggcgctttt cgttataacg gcaattcgct tgtctatttg cctttagata atcccggttt
     1441 aaatccaaaa aatccgcaaa gctcaccgta tgagctgagt agctatgctg tatacagcat
     1501 actgcaggac aagaaaggta atatttggtt cggtacacag gcccgtggtg tgtgtcgctt
     1561 cgatggacaa tcttttacct ggttcactga aaaaggactg ggtggcccgg ccgtactggg
     1621 tttgtttgaa gacagtcacg gaaatatctg gttcggaaat aatggtgccg gattattccg
     1681 gtatgatgga aagaccttaa ccaattttac agcagaaaaa aaactgggta ataatgaatt
     1741 cagccttgca ggtaatcccg gccctggtac ccttgcccgt atttattcaa taaacgaaga
     1801 caagacggga aacctctgga taggaacctt agacgcaggt gcctggagat atgatggaaa
     1861 cactttaacg aattttacga ccaaagacgg actgaccagt aatgcaataa atacaattta
     1921 taaagacaag tttggcgaac tttggtttgg aacggacggt gatggcatgt gccggttcaa
     1981 tggaaagacc tttacagcgt tggttgtaca atagtggtac aataaaacag aagcaatcat
     2041 ctaaaacatt aatacaatga aaacatccaa acatgtatta attgcattgt tgcagttcac
     2101 aattatcagc acaaaagccc aggttggtat tggcactcct gctcctgctg cttctgccca
     2161 acttgatatt tcatctacga gcaagggatt acttgtgccc cgcatgaccc aggcacaacg
     2221 aacaaccatt gcaacccccg atgccggctt gctggtgtac cagacagatg gtgcttctgg
     2281 attttattat tttaaaagtg gtggatggtt acgattggtt gccgaatcat cttatccggc
     2341 agttactatc tgtacgcaat tgtggatgga taaaaacctg gatgtaacca cctatcgtaa
     2401 cggggatatt ataccttatg ttacggacca gacagagtgg gctgcattaa caacaggcgc
     2461 ctggtgttat tacaataatg acccttccag caatgcagta tatggcaaat tatataactg
     2521 gtatgccatc aatgattccc gtgggctggc accggcgggc tggcatgtgg caacagatgc
     2581 agagtggagt acgttgtcaa cctgtttggg cggggcatca gtggcaggag gtaaaatgaa
     2641 ggtgacaggt actacaacat gggcatcacc gaataataat gccagcaata gcagtggatt
     2701 tgcagccctt ccgggtggta tccgtacgaa tggctccagc aactactttg ttgacctaaa
     2761 ttattttagt catttctgga gtgcaacgga acttattccc acaccccaac tcgccttcag
     2821 cagatacata attaactctg gaggtgacct cacacaatat aacgggtttg caaagagctt
     2881 tgggctgtcc gttcgttgta tcagggattg atcattatgc tgtttgatta tttgtcaatt
     2941 gcagtgaata ttattctaca ctttgtaagg aaaaacttct agcggcagat aaacctacaa
     3001 tgaagccttt atagttatac cttgtactat cttgtttggt taatcatttc ttcatgactt
     3061 ccctataaat cccgtttttc agggatagag tagtttaatt aagaaagaca tatttgtact
     3121 taaaacttaa attgtatgaa actgcaatcc ctgatcatcg cactaagttt gctgacaact
     3181 gcaactgcac aaaatgtagg gattggcact accacacctg ttgcccggtt gcatgtagca
     3241 gatagtaatg ttttatttac cgggcctctt acagtacccg gaacaaccac ttatgatcct
     3301 cctgcatccg gcgcaggaag ccgtatgatg tggtatccgc aaaaagctgc attcagggtt
     3361 ggctttgctg agagtacaca ttgggataaa gataatattg gccggttttc tttttcatca
     3421 ggatataatg gcaaagcaat tggatttgct tccaccagta tgggtgttac tacaattgcc
     3481 agtggatttg cttctaccag tatgggatct tttacaaccg ccaatggaga ttattccaca
     3541 agcatgggaa aaaatacaac cgccaatgga atttactcca ccagtatggg atactataca
     3601 tccgccaatg gagattattc cacaagtatg ggaaatttca caatcgcaac atctgagaat
     3661 tcgttagtaa taggtaaata caacgaaaat actaatacta accgtttatt tgaaataggc
     3721 aatggtactg gagttaatgc cagaagcaat gcccttacag ttttggtaga tggcaatata
     3781 ggaattggta ctactactcc cacccgtcca ctttcatttc cacctttttt aggagaaaaa
     3841 atattactct accccggagg gattggtgaa gtaggtatcg gtgtttatgg aaatgaatta
     3901 aggttgcatg cagataaccc cggggctaaa gtaagctttg gcacacaaac caatgccggt
     3961 gcttttacag aagcagggaa atttgaaatt tccggcgggt atgcattaag tgtgttcggc
     4021 agtatctggg ccaatggaac tacctatgcc agtgatgaac gttttaaaca aaatattaca
     4081 gcaataaaat ctccgttaca aaaattactg caattaaatg gagtggaata tgaaatgaaa
     4141 acagatgaat tttctaaaaa ccattttctg ccgggcaggc aaatgggttt gctggcgcag
     4201 aatgtagaaa caatcgttcc ggaggctgtg catgaaaaag atggttacaa gggagttgac
     4261 tatgccaggc tggtgccctt actgatagaa tctataaaag aacaaaataa aaaaatagag
     4321 gaacaacaaa agcaaataga tgaacagaga aaaatgattg aagaactttt aaagaaacag
     4381 ttatgaaaaa aatgtttagc cttgtaattg ttgtttcatc ctgtatgcat gttgaagcac
     4441 aaaatgttgg aataggtaca accacaccct tagcaagatt acatgtaaca gacagtaatg
     4501 tggtctttag tgcaacaggt ttagcagttc cggttccggg caatccgcct gtatccggca
     4561 ccggccggag aatgatgtgg tatgctgaca agggtgcttt tagggttggc tatgcggcag
     4621 gtacaagttg ggatagaaca aatgtgggca actattcttt tgcatccggt tatggctctg
     4681 ttgcttcggg tgattattcc tcagcattag gcttaaataa taaagcagcg ggtatgagca
     4741 gcgttgcgct gggcaacaat gcccaggcaa acacagatta ttccacggcc attggcggtg
     4801 taagttttgc taatgggcta agttcaacag ccatcggctt ccaggctttt gccaccggcg
     4861 actattccac tgtattggga tataacaata tttcaaaagc gctgggtgga attgtaatgg
     4921 gtatgtataa tgatgcaaca gataccccca atcctaatga cacttcttct cttgatagaa
     4981 tttttcaaat aggtaatggc tattatgatg ccatcataga tgatgaagta agaagaaatg
     5041 ccataaccgt tttaagaaat ggaaatacag gcatcggtac atccactcct gcagcctcag
     5101 cagtactcga tgttagttca acaacaaaag ggtttttacc accccggatg acagcggctc
     5161 agcggacggc tattgcaaca ccggctgaag gtttgctggt ttaccaaacg gatgctgtgg
     5221 caggatttta ttatataaga aacgggatat ggacaggatt aaatgatgcc gcaacattcc
     5281 ctgccaatac tatctgcaca caacaatgga tggataaaaa cctggatgta aacacttatc
     5341 gtaatggaga ccccatacct tatgtaaccg accctgttgc atgggctgca ttaacaaccg
     5401 gtgcctggtg ttactataat aatgatccat ccaataatgc aacctatgga aaattgtata
     5461 actggtatgc tgtaaacgat ccaagaggat tagcccctgc aggctggcat gtgcccagta
     5521 ctacagaatg gactacgctg gaaacctgtt taggtggacc aacagtagca ggtggtaaaa
     5581 tgaaagtaaa cggtaccaca acctggcaaa gccccaatac gggagcctct aacagcagtg
     5641 gctttgcagg tcatccgggc ggataccgtg acgaactaaa tgggagttat ggatttattg
     5701 gccaatatgg taactggtgg agttcaactc aaagtggtcc ctcaaacgca tttttccgtt
     5761 acttgtacta tgcggatgct tctttctatg tattcagctt gcctaagcag agaggacagt
     5821 ttgttcgctg cattaaggat tgattattgt tgtactggat ttttataact gccccgaaaa
     5881 gagcttcctt tcttggtaca atggttacag gagttactat taataattct gcttgtaatt
     5941 tgtgagtgga gaaaattcat tttatttaaa acaattcacc cggtttaggt atactaatta
     6001 gctgcattcc tgtacaatcc atggacaatg ccggctgatg gaatatcact catcagcatt
     6061 gatcaacttc aacttcattt ttttaacctg taagccgata ggcaggtgca aaatgtatta
     6121 catcaaaacg ttgattttaa aaggtgatac tttttgagag gtatatctaa tatcataaat
     6181 ttatatattg tgtatcattg aaaaaagaga gaataaataa tgagaatagt tacctggaat
     6241 tgtaatggtg ccttaagaaa taagtttgaa catctttcag aacttaatgc agacctctat
     6301 ataatccagg aatgtgaaaa tccagaatca gcaaaacatc aacaatatat ttcttgggca
     6361 ggaaaccatt tatggaaagg tgacaataaa aacaaaggac tcggggtctt tgctaaaaat
     6421 aaaacaaacc ttgaattaat tgactggtgc aatatttata aaaatcatga ggtaaaacac
     6481 ttcttgcctt gtttggtaaa ttccgatttt caattactta gtatttggac aagtcaaaac
     6541 aaatcaccaa attttggtta cataggacaa ttgtggaaat atttagaaat aaacaagtca
     6601 aaatttaaaa aaataattct agctggtgat tttaacagta acacaatctg ggatgaatgg
     6661 gatagatggt ggaatcattc tgatgttgtg agcgacttat ctaaaattga aatcaacagc
     6721 ctgtatcatc gatattttaa tgaggaacaa ggaaaagaaa ctaaaccaac tttctttctt
     6781 caaagaaata gagcaaaacc ctaccatatt gactacattt ttgcatcaaa agagatagaa
     6841 aataatatga ctaattttga aatagcaact attgataaat ggttaaatta tagcgatcat
     6901 cttcctgtta tttgtgaaat aaatacttta taaatgaaat tcagtaatac aataaaatat
     6961 tccttattga cggagaacca aatcaaatag gttttcccaa taaatacccg agattctaaa
     7021 cagttaaaat atatgctcac ctgtatacga accaattctt cccaaaatca ttttcagcaa
     7081 ctcgtcaggg aactggatgc agacctgagc gtaagggatg gcgaggaaca tgccttctat
     7141 gcccaattca ataaaacaga caggataaaa catgtggtgg tggcgtatga taacgaaaca
     7201 ccggttggtt gcggcgccat caaagaatat gcagcagata ccatggaagt aaagagaatg
     7261 tatgtacccc tgcaccggcg gggacagggc attgcttcac ttattttaga ggagctggaa
     7321 aaatggtgcc gggaacttaa ctataacaag tgcatattag aaacaggtat ccggcaaccc
     7381 gaagccatcc gcctttatga aaagaacggt tatgcccgca tccccaacta tggccaatat
     7441 gccaacatgg acaacagtgt atgctttgaa aagaaattgg gttgatcagc ttgctacggg
     7501 cctgccgcta ctaacttctt cattcccaaa ctactgcttt actcaaagtt gagcctgtac
     7561 aaagctctga aacccttacc tttgcggcaa tttattttta agttcttatt tctaaaacaa
     7621 gcagttatgg cagatatttt tgaacgttta ctgaagaatg ccggccctct tggtcaacat
     7681 cgtgaaaggg cacacggcta ttttgctttc cctaaactgg aaggagagat cagcagccgc
     7741 atgaaattcc gcggtaaaga catgatcgta tggagcctga acaactacct gggcctggcc
     7801 aaccaccccg aggtacgaaa agcagatgca gaaggcgctg cccaatacgg actcgcctac
     7861 cccatgggtg cccgtatgat gagtggcaac agtaacctcc acgaacaatt agaagctgaa
     7921 ctcgccgctt ttgaacataa ggaagatgcc gccctgctga actacggtta ccagggaatg
     7981 gtcagtatca ttgatgtact gtgcagccgt catgacgtga tcgtctatga tgccgaaagc
     8041 catgcctgca tcattgatgg tgttcgttta cacccgggcc accgtttcgt tttcaagcat
     8101 aacgaggtgg aggattgcga aaaacaatta cagagagctg cagccttgat agaaaaacaa
     8161 aaagccggtg gtatattggt tatcacagaa ggtgtgtttg gcatggccgg cgaccagggt
     8221 aaattgaaag agatcgctgc gctgaaagaa aaataccagt tccgtttatt ggtggatgat
     8281 gcacatggct ttggcaccct cggcaaaaca ggtgccggtg ccggtgaaga acagggtgtt
     8341 caggataaaa tagatcttta cttctctacg tttgccaaat caatggcttc aataggtgcc
     8401 tttgtggccg gtgacaaaaa gatcattgat tttatacggt ataatatccg ttcacaaatc
     8461 tttgccaaga gcttacccat gccattggta atcggaaacc tgaagagact ggaactgtta
     8521 cgcaatcacc ccgaactgaa agagaaactc tggagcaatg cccgcaaact gcagaacggt
     8581 ttaaaagaaa gaggatttga tataggtaat actgattcag tggtgacacc ggtgtatatg
     8641 aaaggggatg tacccgaagc tactgcaatg gtgatggacc tgcgggagaa ctacggcatt
     8701 ttcgcctcca ttgtagtgta cccggttatt ccaaaaggac atattattta ccgcctgatc
     8761 ccctctgcaa ttcataatga tgaggatatt gaactgacac tgaaagcttt ttctgaaacc
     8821 aagaaaaagc tggatgccgg tgaatataaa gtggaagcaa ttcccgacat ggcggaaacg
     8881 aaataagcaa tataattttt catcctgagc ctggcaagga tgagttttta aaaatcgagg
     8941 ctgtctcaag aacaaacaaa tttgtcacat tgagtttatc gaaatgcgta caaatttgtt
     9001 taccaatcat tgtcatattt cggctttgct caatatgaca atgattggga cttttgagac
     9061 agcctcgtct tttatttgta cggcagcacc ttttggcctg tttaccctac atttaccctt
     9121 taaaaattac cacattatgc gtaagctttt tctgctactc tttctgctgc cggtaatggc
     9181 cgtagcacaa aagaaatcca tcacgttgga agacatttac aaaaagggaa ctttccgcgg
     9241 cgaaccggtc agggctgttt ttgatcaaac cacaaaagaa ccggaactga aactggaagg
     9301 cctgaaagat gagagcggca aaacattcgg tcaaccggaa gatgtgatca cctctgctgc
     9361 taaccctgtt gttgctatcc tgaaaaaagg tacagaaccc atttaccgcc gttcttccaa
     9421 atcatacata tacatatacg atacaacaac taaaaaactg gtaaagccgg agaatgaaaa
     9481 agtattgcat cccaccttct cgc
//
LOCUS       DAORTI010000127         9488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7734_length_9488_cov_7.849889, whole genome shotgun sequence.
ACCESSION   DAORTI010000127 DAORTI010000000
VERSION     DAORTI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_9488_cov_7.849889"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..2407)
                     /locus_tag="PLO70_13470"
     CDS             complement(86..2407)
                     /locus_tag="PLO70_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQZ75524.1"
                     /translation="MWYRLGQFILKYRLPLLIILAALTGFMAYHASKVQLSYDFTRAI
                     PLNNPKYKAYQEFRKKFGEDGNLLVIGIQTEKLFEEKTFNAYYDLQRELKKIKGVDDI
                     ISIPSAINLVKVPETEKLKADTIFPDRILSQAAIDSASAVFFNLPFYEHLLYNPETKA
                     WLMGLRINKDLMATKARVAVVASISKLADDFGKENNLTVYKSGLPLIRTEIAVMIANE
                     MKFFLLASVVLSALILLLFFRSFSVMVLSLVVVAIGVVWSMGTIQLLGYKISLLTALI
                     PPLVVVIGIPNCIYFFNKFHVAWNETGNKKGSLVAMVDKMGIVTLFCNVAAAIGFAVF
                     ALTQSQILKEFGLVAGINIMALFFISLVLIPAVLSFLPAPKSRHTKYLDNPRLNRWLD
                     RLERWSLNHRKLIYSITGVVVAFSVVGIFQLKSEGFIVDDLPKSDKIYTDLKFFEKNF
                     KGVMPLEIVVDTKKKQGVTRNLNNLERIDSLSQYLATIPDIARPLSIAEGLKFAKQAF
                     FDGDKANYSMPSSYDLPALSQYLSFKGDSANGKNSLAKLVSTFMDSTKQQARISVSMA
                     DIGSHRLPGLLDSITVRADQLFDKEKFDVQLTGTSVTFLEGSRFIINGLKESIFWAFL
                     LIALCMFYLFRSARILICSLIPNIIPLMITAGVMGWVGIALKPSTVLVFSVALGIAID
                     VTIRFLVNFKQELPHYKYDMKQTVIETIHSTGISIIYTSMVLIAGFVIFYFSEFGGTK
                     ALGWLTSLTLITATFTNLVLLPAILISFIRIKK"
     gene            2489..4789
                     /locus_tag="PLO70_13475"
     CDS             2489..4789
                     /locus_tag="PLO70_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ75525.1"
                     /translation="MRYYLVIQFLLVAAATIAQDPVPPIGQWREHLPYNSAIDVTAGN
                     GKVYCATPYSLFSVNTSTNEIERYSRVTGLNETGIRTIKYDETNNKLFIAYQNSNIDI
                     LYRNDIYNIPDIKRDNVIGDKNIYNIYPLGKNYYLSTGLGVIVIDGDRYEVKDTWLIG
                     SGGSQVKVNGFASDSSFYYAATEEGLKRVAMNSTDPANYSNWQSVSGINGLPTGACRN
                     VFSIQDKIVVEKNDSLFVQSGISWTLLYTDGWPFVSSNTTENKIQLCQRQPNGISRVV
                     ILNADGTIFRTLANVGAVSFPRKAIFLNNDPWVADQFASLSHFGSSLSYEQYSLNSPQ
                     AIASGEMVVYNSVFYATAGSVNDAWNYQYNGDGIYSFKEGTWTNVNRYKYSQIDSLLD
                     YVTIAVDKRDETFWAGSYGGGLLHVKTGPSFEIFKQNYISPAIGDPASYRVAGLAFDG
                     ENNLWISNFGSNQPLRVLKNNGSWINFSLPFPLFENAVSQIVIDDNNYKWIVAPLGNG
                     LICYDHGASIDNTGDDRWRRFFSGTVNGNLPSSEVTCVAKDKNGFIWVGTTDGIGVIQ
                     CPEQTFGAPACAATWPIVSNGNFAGYLFKGQEVRSIAVDGADRKWVATRNGVFLINAT
                     GEKVINQFTENNSPLLSSDVKKITIDGKTGEVYFATLKGICSFRSTATEGAETNEEII
                     VFPNPVPPGFAGTIGIRGLANNAIVKITELDGRLVYQTRALGGQASWDGRNYRGQKIS
                     SGVYLILVSDDGRKERTAARIFFIQQ"
     gene            4790..5536
                     /gene="recO"
                     /locus_tag="PLO70_13480"
     CDS             4790..5536
                     /gene="recO"
                     /locus_tag="PLO70_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQZ75526.1"
                     /translation="MPDKLHKTKGIVLKTVKYGETSIIVTIFTELFGVQSYLVNGVRT
                     STKKGTGKANLFQPSAILDLVVYHNDLKHLNRIKEFKWATIYQHILSDVSKNAVALFM
                     VELLTKCLKQPEGNPDLFHFTEDCFLYLDKCNGAVMANLPLFFALHLAHFFGFRMTDD
                     YSTESAFLDLMEGNFVQEQPHHPHFLEDKQAFVTSQLLKAQQPGELEEIKLNHDFRRQ
                     LLHVYETYYALHIPDFGTMKSLPVLRVVLG"
     gene            complement(5528..>6463)
                     /locus_tag="PLO70_13485"
     CDS             complement(5528..>6463)
                     /locus_tag="PLO70_13485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ75527.1"
                     /translation="LCSVVTKRDFSPDIIRQEIIKAEKEMYDNGIVAIGDIGNTADTA
                     EVKSKSKIRWQNFVEVLSFTDDKAEENFNHYKKVAAHLDATLHTAGNIHRTSLVPHAP
                     YSISPKTFQLINESTKGQVISIHNQEHPAEDELYKTGGGEYLKLFKIFGIDRSPFPVT
                     GKSSIRSVLPYFNNEQTILLIHNTFMTEEDVIWANKYAAAHGLKLVYCLCINANLYIE
                     NKVPPIDMLMKHNCTIVVGTDSYSSNWQLSIAKEMEAIHKHFQQIPIETILQWATSNG
                     AKALGLEDEFGSFDTGKKPGIAIIDNNLELSRKLA"
     assembly_gap    6464..6563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6564..7607
                     /gene="prfA"
                     /locus_tag="PLO70_13490"
     CDS             <6564..7607
                     /gene="prfA"
                     /locus_tag="PLO70_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQZ75528.1"
                     /translation="IKARFDQLGVALTNPEIVGNNKKFAETSKEYRSLEKIVVAYNEY
                     KKVLDDIGFYKEALEGGDEEMRELAKMEAPAMEEKKTQLEAHIRQLLIPKDPQDEKNA
                     ILEIRAGTGGDEASLFAGDLLRMYVRYCERRGWKTAVLSENEGSVGGYKEVQLEVTGD
                     DVYGTLKFESGVHRVQRVPDTEASGRVHTSAATVAVMPEAEEVDFELNPADVKMETAR
                     SGGAGGQNVNKVETKVLLTHIPTGTVVVCQTERSQLGNREKAMGMLRTKLYEEQVRKQ
                     EDEIARHRKSLVSTGDRSAKIRTYNFPQGRVTDHRIGLTLYNLDAVLNGDINELIEAL
                     QFAENSEKLTKQN"
     gene            7749..8384
                     /locus_tag="PLO70_13495"
     CDS             7749..8384
                     /locus_tag="PLO70_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75529.1"
                     /translation="MLDQLLDIVKNYGKDTVVENPEIPNEQNQEVLADATKTITSGFQ
                     NVLAGGGLQNILDLFKGGDKSSGNSGGISGLTKNPMVSMMVGYFISKLVSKYKMSPTS
                     ASNVANNLIPNVLNGIITETNNPQNAGFTLDGLIGSLTGGSGNGGGSQLQDILDQFTG
                     GGGNSNGNNSGGFDLQDLIGNFTRKAQDSFQNKDQGSGGGLMDLIKGFLSK"
     gene            complement(8818..9201)
                     /locus_tag="PLO70_13500"
     CDS             complement(8818..9201)
                     /locus_tag="PLO70_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQZ75530.1"
                     /translation="MKKIKPSFIPGITWLIISTILLTLPGSAFPTENWLGKIWFDKWV
                     HIGMFSIMVVLWCWGLLKLNFDTSGLKKNFILISVVWLLYGIGMEFIQKYFVINRSFD
                     TGDIIADGAGCLAGLFFSLSRYIKK"
BASE COUNT         2861 a         1736 c         2097 g         2694 t
ORIGIN      
        1 cctttgacaa ttaaccagaa ccaattgctg cacttatccg gtagatttta tggtcaaaaa
       61 gtccttgtaa aaatagggaa atagcttatt tcttaattct aataaatgaa ataagaatgg
      121 ccggcaataa taccaggttg gtgaaggtgg cagtaatcaa tgtaagcgaa gtaagccagc
      181 ccagggcttt ggtgcctccg aattcgctga aataaaaaat gacaaaaccg gcgataagca
      241 ccatggaagt atatatgata ctgatacctg tgctgtgaat agtctcaatc accgtttgtt
      301 tcatatcgta tttgtagtga ggaagctctt gtttaaaatt tacaaggaaa cggatggtga
      361 catcaatggc aatacccaag gccacgctga aaaccaatac agtagagggt tttagtgcaa
      421 tccctaccca acccataaca ccggcagtga tcatcagggg gattatattg gggataagcg
      481 aacaaatcag gatcctggct gaccggaaca ggtaaaacat acacagggca atcaacagga
      541 aagcccagaa aatactttct ttcaaaccat tgataatgaa acggctgcct tccaaaaaag
      601 tgacactcgt ccctgtcagt tgtacatcaa atttttcttt atcaaaaagc tggtctgctc
      661 ttacagtaat actgtcaagc aacccgggaa ggcggtgact gccgatatcg gccatactaa
      721 cactgatgcg ggcctgctgc ttggtgctgt ccataaatgt gcttaccagc ttagctaatg
      781 aatttttgcc attcgcagag tcacctttga aactaaggta ttgagaaaga gctggcagat
      841 catagctgga aggcatagaa taattggcct tatcaccatc aaaaaatgcc tgtttcgcaa
      901 atttcagtcc ttccgcaatg ctcagcggcc gggcaatatc cggaattgtc gccaggtatt
      961 gtgataatga atcaattctc tccaggttat tcaggttacg ggtcacaccc tgtttctttt
     1021 ttgtatcgac cacaatttcg agtggcatca cccctttaaa atttttttcg aaaaacttca
     1081 ggtccgtata gatcttatct gatttaggca gatcatcaac aataaatcct tcacttttta
     1141 attggaatat tccaacaaca gaaaaagcca cgactactcc ggtgatactg taaatgagct
     1201 tccggtgatt taacgaccat ctttccagcc ggtcaagcca tctgttaagc cggggattat
     1261 ccaggtattt ggtatgcctc gatttcggcg ctggcagaaa acttaaaacg gccggaatta
     1321 aaaccaggga aataaaaaaa agtgccatga tatttatacc cgctaccaaa ccaaattctt
     1381 tcaaaatctg gctttgcgtt aatgcaaata ccgcaaaacc gatagctgct gcaacattgc
     1441 agaaaagcgt aacgattccc attttatcaa ccatcgctac gagtgagccc ttcttattcc
     1501 cggtttcgtt ccaggcaaca tgaaatttat taaagaaata aatgcaattc gggatgccga
     1561 ttactaccac caatggtgga atcagggcgg ttaaaagact gattttataa cctaacaact
     1621 ggatagtgcc catactccac accacaccaa tagcaaccac gaccaacgaa agcaccatta
     1681 cgctgaaaga acggaagaaa agcaatagaa ttaaggcaga gagcaccacg gaagccagta
     1741 agaaaaattt catttcattg gcaatcatca cagcaatttc agttcgtata agcgggagac
     1801 cactcttgta aacagtaaga ttattttctt tgccaaaatc atccgccagt ttactgatag
     1861 atgccaccac agctaccctt gctttggtgg ccatcaggtc tttattgatc cggaggccca
     1921 tcagccaggc ctttgtttcc ggattgtata aaagatgctc atagaaggga aggttgaaga
     1981 atacagcaga tgcactgtct attgccgcct gcgaaagtat tctgtcagga aagatagtgt
     2041 cagcttttag tttttccgtt tccggtactt tcaccaggtt gatagctgaa ggaatagaaa
     2101 taatgtcatc tactcctttg atctttttaa gctctcgctg caggtcgtaa taagcattga
     2161 atgttttttc ttcaaacaat ttttccgttt gtatgccgat gacaagcagg ttgccatctt
     2221 ccccgaattt ttttcgaaac tcttggtatg ctttatattt tggattatta aggggtattg
     2281 ccctggtaaa atcgtagctc aattggactt tgctggcatg gtaagccata aaaccggtca
     2341 gcgccgcaag aattatcaat aacggtaaac gatatttgag gatgaactgt ccgagcctgt
     2401 accacataag aagatgtgca attgtgaggg tgcaaagaaa ggcaaaaaag ctcaatagga
     2461 acatgactgt ttgtgatact tttgataagt gagatattat ctggttatac aatttttact
     2521 tgttgctgct gcaactattg cccaggatcc cgttcctcca atcgggcaat ggcgggagca
     2581 tttgccgtat aatagtgcta ttgatgtaac tgcaggtaat ggcaaagtgt attgtgctac
     2641 tccctatagt ttgttttctg tgaatacttc cactaatgaa attgaaagat acagccgggt
     2701 tacaggattg aatgaaaccg ggatcaggac tattaagtat gatgagacaa ataacaaact
     2761 gttcatagct taccaaaaca gtaatattga tattctctat cgcaatgata tttataacat
     2821 acctgatata aaaagggaca atgtgatcgg tgataagaat atttataata tttaccccct
     2881 tgggaaaaat tattacctgt caaccgggtt aggtgtaatt gttattgatg gtgaccggta
     2941 tgaagtgaaa gacacctggt tgatcggcag tggcggcagc caggtaaaag taaatggctt
     3001 tgcaagcgac agcagttttt attatgcagc gacggaggaa ggattaaaga gggtggcgat
     3061 gaattctact gaccctgcca attattccaa ctggcaatcg gtaagcggta ttaatggatt
     3121 gccaaccggg gcctgcagga atgtttttag tattcaagat aaaatagttg tggagaagaa
     3181 tgattctttg tttgtacaga gtggtataag ctggactttg ttatatactg atggctggcc
     3241 ctttgtaagc agtaatacaa cagaaaataa aatacagtta tgtcaacgtc aacccaatgg
     3301 tatcagcagg gtggtaatac tgaatgctga cggcacaatt ttcagaaccc tggcaaatgt
     3361 tggggctgtt tcttttcccc ggaaggcgat ttttttgaat aatgatccct gggtagcaga
     3421 ccagtttgct tctttgtccc attttgggtc atcattgtct tacgaacaat atagcctgaa
     3481 ttcaccacag gctattgcaa gcggtgagat ggttgtttac aatagtgtct tctatgcaac
     3541 tgccggttca gtaaatgatg cgtggaatta ccagtataat ggagatggaa tttatagttt
     3601 taaagaaggt acctggacta atgtcaatcg ttataagtac agtcaaattg attcgttgct
     3661 ggattatgta acgattgccg ttgataaacg ggatgaaacc ttctgggcag ggtcgtatgg
     3721 cggtggatta ttacatgtaa aaacgggtcc ttcatttgag atattcaaac aaaattatat
     3781 cagtcctgct attggtgatc ctgccagtta ccgggttgcc ggcctcgcat ttgacgggga
     3841 gaataattta tggatttcga atttcggttc caaccagccc cttcgtgtgc taaagaataa
     3901 tgggagctgg atcaacttct ctcttccgtt tcctttattt gaaaatgcag tttctcaaat
     3961 tgtgattgat gacaacaatt ataaatggat tgtggcgcca ttgggtaatg ggttaatctg
     4021 ttatgatcac ggtgcttcta ttgataatac aggagatgac cgttggagaa gatttttcag
     4081 tggtaccgta aatggcaatc ttcccagctc tgaagtgaca tgtgtggcta aagataaaaa
     4141 tggttttatc tgggtaggga ctaccgatgg tattggtgtg attcaatgcc cggagcaaac
     4201 tttcggcgca cctgcctgtg ctgctacctg gccgattgtt tccaatggga attttgccgg
     4261 atatttattc aaagggcagg aggtgagaag tattgctgtg gatggagcag acaggaaatg
     4321 ggtggcaaca agaaatggag tatttcttat caatgccacc ggagaaaaag taattaacca
     4381 gtttacagaa aataacagcc ctttgctgag tagcgatgta aagaagatta cgattgatgg
     4441 aaaaaccggc gaagtatatt ttgccacact gaaagggatt tgttcctttc gcagcacagc
     4501 gactgaaggc gctgaaacga atgaagagat aatagtattt ccaaatcctg taccaccggg
     4561 ttttgccgga acaattggta tcaggggcct ggcaaataat gccattgtaa agattactga
     4621 actggacggc cggttggtat atcaaacaag ggctttgggc gggcaggcat cctgggatgg
     4681 aagaaattac aggggacaaa agatttcaag tggtgtttat ttgattttgg tgagtgatga
     4741 cggacggaaa gaaagaactg cagccaggat tttctttata caacagtaga tgcccgataa
     4801 actacataaa acaaaaggca ttgttttgaa aacagtgaag tatggtgaaa caagtatcat
     4861 cgttaccatc tttactgaat tatttggtgt acaatcatac ctggtgaatg gagtaagaac
     4921 atcaacaaaa aaaggaaccg ggaaggcaaa cctatttcag ccatcagcta ttttagattt
     4981 ggttgtatat cataatgatt taaaacacct gaaccggatt aaagagttta agtgggcaac
     5041 gatctatcag catattttat cagatgtaag taaaaacgct gtggcattat tcatggtaga
     5101 gttgctgact aaatgtctga aacaaccgga aggtaatcct gatctttttc actttacgga
     5161 agattgtttc ctgtatttag ataagtgtaa tggagcagtg atggctaacc tgccattatt
     5221 ttttgcactg catcttgctc acttctttgg tttccggatg actgacgatt acagtactga
     5281 atctgcattt ctagatttaa tggaaggaaa ttttgtacag gaacaacccc atcatccgca
     5341 tttcctggag gataaacagg cttttgttac ctcgcagtta ttaaaagcgc aacaaccggg
     5401 ggagctggaa gaaataaaac tgaaccatga ttttcgccgg caattattgc atgtatatga
     5461 aacctattat gcgctgcata ttcctgattt tggtacgatg aaaagtttac ctgtcttgcg
     5521 ggtggtatta ggctagtttc ctggatagtt ccaaattatt gtcaattata gctattccgg
     5581 gttttttacc agtatcaaag ctcccaaact catcctcaag gcccaaagcc tttgccccgt
     5641 tactggttgc ccattgaagg atcgtttcaa tgggaatttg ctggaaatgt ttgtgtatag
     5701 cttccatttc tttagcaata ctcagttgcc agttgctgct gtagctgtcg gttcctacaa
     5761 cgatagtaca attgtgcttc atcagcatat caatcggggg tactttattt tcaatataca
     5821 ggttggcatt gatacataaa caataaacca gttttaaacc atgagcagca gcgtatttgt
     5881 ttgcccagat cacatcttct tctgtcataa atgtgttatg gatcagcaaa atagtttgtt
     5941 cgttgttaaa gtaaggtaga acagaacgta tactgctttt acccgttacc gggaatgggg
     6001 atcggtcaat tccaaaaatt ttaaatagtt tcaggtattc accaccaccg gttttgtata
     6061 actcatcttc agccgggtgc tcctggttat gaatggagat tacctgtcct tttgttgatt
     6121 cgttgatcag ttgaaaggtt ttgggagaga tactgtaagg tgcatgagga accagggagg
     6181 tacgatggat gttgccggct gtatgaagtg tggcgtctaa atgcgctgct acttttttat
     6241 agtgattaaa attttcttct gctttatcat ccgtaaaact taatacttct acaaaattct
     6301 gccagcggat tttgcttttg ctttttactt cagccgtgtc agcggtgttt ccaatatcac
     6361 caatagcaac tatgccgtta tcatacattt ctttttccgc cttgatgatc tcttgccgta
     6421 taatgtcagg agaaaaatct cttttggtga caaccgaaca taannnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnataaaag caagatttga ccagttggga gtagccctta
     6601 ccaaccctga gattgtgggg aataataaaa aatttgctga gacaagtaag gaataccgca
     6661 gcctggaaaa gatcgttgtg gcatataatg agtataagaa agtacttgac gacatcggtt
     6721 tttataaaga agcattggag ggtggagatg aagagatgag ggagctcgcc aaaatggaag
     6781 ctccggcaat ggaagagaag aaaacacagc tggaagccca tatcaggcaa ttgctgatac
     6841 ctaaagatcc gcaggatgaa aagaatgcta ttcttgagat aagggctgga accggtggtg
     6901 atgaagccag cctgtttgca ggagatttac tgagaatgta tgtgcgttac tgtgaaagac
     6961 gtggttggaa aactgcagta ttgagtgaaa atgaaggctc ggtgggtggt tataaagaag
     7021 tacaactgga agttaccggc gatgatgtat atggtaccct gaaatttgaa agcggcgtac
     7081 accgggtaca aagggttccg gatacagagg ctagtggcag ggtgcacact tctgctgcta
     7141 cagttgcagt gatgcctgag gcggaggaag tggattttga gttaaatccg gccgatgtaa
     7201 aaatggagac agcccgaagc ggaggtgccg ggggacaaaa tgtaaacaaa gtagaaacaa
     7261 aagtattact gacacatatt cctaccggca ccgtagtggt ttgtcaaaca gaacgttcac
     7321 aattgggtaa ccgggaaaaa gcgatgggga tgcttcggac caaactttat gaggagcagg
     7381 taagaaagca ggaggatgag attgcccggc atcgtaaaag tttggtaagt accggtgacc
     7441 ggagtgcaaa aatccggacc tacaattttc ctcagggacg agttaccgat catcgtattg
     7501 ggctcacgct gtataatctt gatgctgtgc tgaatggaga tataaatgaa ctgattgagg
     7561 ctcttcagtt tgcagagaat tcggaaaagc ttaccaagca gaattaatga aaaaatacaa
     7621 ttccggacac ccatcaacag aatattacct gctgtcatga tttcttgaga ttatttataa
     7681 ttaattttag tgaaaacact taaataatct tacctgagag aatcattcat attcaaaaac
     7741 tataaattat gttagatcaa ttgttagaca ttgtaaaaaa ctacggaaaa gacaccgtgg
     7801 tagaaaaccc ggaaattccg aatgagcaaa atcaggaagt attggctgat gctactaaaa
     7861 ccataaccag tgggtttcag aatgtattgg ccgggggtgg acttcaaaat attcttgatc
     7921 tttttaaagg aggtgataaa tcatctggga acagcggtgg tattagtggc ttaacaaaaa
     7981 acccgatggt ttccatgatg gtggggtatt ttatcagcaa actggtcagc aaatacaaaa
     8041 tgagtccaac ttcggccagt aatgtggcta ataacttaat tcctaatgta ctcaatggaa
     8101 ttattactga aacaaataac ccccagaatg cagggtttac tttggatggc ctgataggtt
     8161 ctttgacagg cggaagcgga aatggcggcg ggtctcaatt acaggatatc ctggatcaat
     8221 tcaccggcgg aggtggaaat agtaatggta acaatagcgg tggatttgat ttacaggatc
     8281 ttattggcaa ttttacccgc aaggctcagg atagttttca gaataaagac cagggtagtg
     8341 gcggcggact tatggatttg ataaaggggt ttctgtctaa ataatttcaa attgaattaa
     8401 ctaagtcatc ctttttcaga ggatgacttt tttattttct gtgaaataac aatttggcat
     8461 gaattttccg aaggaaacag tcaggcacaa cgtctaatag gtgtatgttc tttgtcagtt
     8521 gattgtcagg tttttttaga ttgacagaat gaacaaatac taacatctgt tgttatttgt
     8581 gtaatatgtt caatcagtgc atcgtatgcc tgacaggtgc aaaagagatt ttacgaagaa
     8641 agaagtgagt ggaattactg atttagctct tttaaacttt cattaacagc aaagttcata
     8701 ctatcggcag taaatttgcg ttatacattt gaagattatt atttgaactc attataaaga
     8761 cagtcaattt tctcataagc agttagtttt ggttgcgacc cctgtttcta caggggtcta
     8821 tttttttata tacctgctca aactgaaaaa aagaccagcc aggcagccgg caccgtcagc
     8881 aattatatct cctgtatcaa aagatcgatt gattacaaag tacttctgga tgaactccat
     8941 ccctattcca tagagaagcc atacaacaga aataagtata aaatttttct tcagtccgct
     9001 ggtatcaaaa ttcaacttta gtaatcccca gcaccacagt actaccatta tggaaaacat
     9061 gccaatgtgg acccatttgt caaaccatat ttttccaagc cagttttcag taggaaaagc
     9121 tgagcctggc aaggttagca aaatggtgga aatgatcaac caggtaatac ctggaataaa
     9181 ggaaggtttt attttcttca aacgtttttt ttcaacaata ttaagcgaag attcgctgtc
     9241 attctttatt ttaaaaaaga aaaaagagcc tcagaagagg ccccttgttt ttaaccaact
     9301 atagaaccaa aactactata gcagcgtttg tatcttatca gatacgataa aacgtctgtc
     9361 gtcagattga aagaaaatgg aaaaatcaga gtttacccat cacatacatt gcaccctggg
     9421 gagtgggatt gccgccttta ttttcaaggg tgatggcaaa cgcttcggcc ccttgtgcat
     9481 tcttcatg
//
LOCUS       DAORTI010000128         9468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7771_length_9468_cov_7.826835, whole genome shotgun sequence.
ACCESSION   DAORTI010000128 DAORTI010000000
VERSION     DAORTI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7771_length_9468_cov_7.826835"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(491..7831)
                     /gene="sprA"
                     /locus_tag="PLO70_13505"
     CDS             complement(491..7831)
                     /gene="sprA"
                     /locus_tag="PLO70_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQZ75531.1"
                     /translation="MNLRTAHIFRTLVVFTMVLASCLQASAGDFKPVFNNRNLFLDDT
                     TRYPIRDRYGDPYSNPSRNTFDLRDTAFIKRTIEYDPKTKQYYIVEKIGNRYYRTPAS
                     FTMEEFVRMQGKKDEEDYFKKRASLMSNMNRRMVRPKFKVSNDWFNRIMGVGPDGKVK
                     IEIKPSGYVDFLAGYQGQNIKNPTLPERARRNGGFDFNMNSQLQMDAQIGEKLKLPIN
                     YNTLANFNFENQLKLDFRGKDDDIVKMFQAGNTSFASKGTLIPGAQSLFGIKTQLQFG
                     KLFITTVLANQQSQRQTMGLQGGSAAQRFSLKADEYDENRHFLMSQYFRSNYNTAMKQ
                     LPIVNSKVQVLRVEVWVTNRNGSTTDTRDIVAFMDLGEGNPYSPILTGTGDGLPRNDA
                     NSLYSSLTTNPNFRNSSLVQNQLTTFGLSPVQDFEKTFARKLQPTDYYFNPQIGFLSL
                     NQPLQPDEVLGVAFQYTYNGRVFQVGEFSQDVPPDTTGNSQRILFLKLLKATSQRTNL
                     PIWELMMKNVYSVGFGQLERQDFMLDMLYEEPSFGEKRYLPPTDVVDPYKGQPIISLV
                     NLDRLNNQNDPQPDGVFDFIEGYTVISSQSRIIFPVLEPFGRDLDYIYPSQAIRDQYL
                     YYPLYDTIKAIAQTYANLNRFKIVGKSKSSGFGSGGGGSEYQLGYNIPRGSVTVSAGG
                     QVLVENVDYEINYDLGTLRVVNQAIINSGVPVNINYENNQAFGFQQKNFLGLRLDYMA
                     SNKLTLGGTIVRLGERPFFIKQSYGEDPIKNTMYGFDLDYRNNLPRLTKLLDKLPFYS
                     TKAMSSITTYAEAAVLQPGHARQINSGNQDRSGQVYIDDFEGTRAGIDLRFPLISWTL
                     ASIPQGNGLFPESGLNNDLRSGYNRGKLAWYNIEPVLQERRNSNNPLANDPEELSRPE
                     TRQVLQKEIFPRRSTQFGEGLLTTFDLAFYPKDLGPYNFEFDPNRVDANGKLRNPKQA
                     WGGMMRNIDQTDFETGNIEYIEFWLQDPFIANTANPNGGQLYFNLGNVSEDVLRDGKR
                     QYENGLPTPTNNARVDESSVWGKVPAIPLQVTNAFSNTATDREFQDVGFDGLQDTAEQ
                     RIFTPYLNSLQSNFGTNSPIYQKAQADPSGDNFRAYRDPAFDQQPVTGILQRYKNINN
                     PHGNSPIAENNTQFVNAFTQYPDAEELNRDNTMNEVEEYFQYKVDIRPNMAAGTNFIT
                     DVRTPTVTLANGQSRTEKWYLFRIPVDQYQQKIGNIPDFKSIRFIRMFVTGFEDSVVM
                     RFGKLELIRNQWRKFKNKIDTTGNYTTLPAPDPTTVNVLAVNVEENDQRQPIVYRIPP
                     GIERQQQLSNNNVALFLNEQSISMQVCGLPQFESRGVFKTMNLDMRQYGRLSMFIHAE
                     SVQNAQPIEDGDLNAVVRIGNDFSGNYYEVRIPLKKTDFGETDSLKIWPEENNLDFSL
                     DDLTDLKTKRNKLGISPSQYYKEVLPNGRSYAILGNPNLGEVRGMLMGVENVTQESVC
                     TEVWFNELRLQQLDEKGGWAALARADIKLADLGMISISGTAKSRGFGTLEQRVNERSR
                     EDVYTIDASATIEAGKLLPKKLGLQIPIYAGISRRASTPEYDPYDLDLDLKQKIKDAD
                     ADKRDSIKNDAQDVTTIKTLNLTNVRKLKTDGKKPKPWSITNFDFNYSYIQTLSHNPL
                     IERDEMRRTRGAIGYTYAPQTKPFEPFKKLIKSKSPWLSLIKDINLNYAPNQVSFRAD
                     VFRQFGATRPRNVGGGPYQIPETYNKYFTFDRFYILQWNLTRSIAIDFSATNNARIDE
                     PIGRIDTKLKKDTVRKNLFKGGRNTTYSQDVTISYNVPTNKIPLLDWTTLRANYNTKY
                     NWLASSLLARNLGNTLSNTQTRTINGELKFEELYNKWRFLRAVNTSSPNAGAGNKAAG
                     GGKNQKKGDNKNTDGKTGDDTKNENQVKNQQQVNTEGGIRIDTIRNKKGKIIRIKKRK
                     IKAKKDPNQLPEISNVPKFFLRLATSLKRVGIQYTEDFGTTLPGYMDSTQLLGNNFKS
                     REPGFGYIFGYQPDTNWINNFGAKGLLSRDSLVSAMIQQRYNQRLNITAQVSPFRDFN
                     IDINLDKTFDKQYSELYKDTTGSAGLTRLNPYALGSFSVSYISYQTLFKKFDPNVVSE
                     TFKQFEANRRILSGKLGGLNPYQTGGTDPDGYVKGYGRYAQDVVIPAFLAAYTNKDPL
                     GVKLTKNSNPNIKANPFSGILPKPNWNITYTGLSRMKGLDKIFTNFTIRHGYRSTFSM
                     NSFNTALLFADPFRVGYPSFRDTLTGNFIPYFLVPNITIQESFDPLIEIDITFTNQLT
                     TGFQIKKSRTLSLSLIDYQLAENRSTEVTFNFNWRKKGIPLIKNIKIGKNGMKLDNDV
                     TFRMDFSLRDDATANSKLDQGTAFGTGGQKVIRIAPSIDYILNNRISMKLYFEQNRNI
                     PKISSAFPITNTRGGLQVRISLAQ"
     gene            complement(7868..8449)
                     /gene="ruvA"
                     /locus_tag="PLO70_13510"
     CDS             complement(7868..8449)
                     /gene="ruvA"
                     /locus_tag="PLO70_13510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQZ75532.1"
                     /translation="MIAFLKGDFVHKTPALLHVEVAGVGYEVQISLNTYSKIQNLDKG
                     VLYTSLLIREDAHILYGFFDLAEKEMFLQLLSVSGIGASTARVMLSYMKPEELAKAIV
                     QGDSKTLEGIKGIGKKTAERMVLELRDKLAKHPLESNISVLKNNTLHQDALNALTALG
                     INRQAASQAIEKTLAANPNVSVEELIKMALRTL"
     gene            8638..>9468
                     /locus_tag="PLO70_13515"
     CDS             8638..>9468
                     /locus_tag="PLO70_13515"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQZ75533.1"
                     /translation="MNKKITAAITAVGGFVPEDKLTNFDLEKMVDTNDEWIRTRTGIS
                     ERRILKGEDKATSDMVVPAVQELCKKRGISPEEIDCLIVGTVTPDMVFPSTANIACDK
                     LGAKNAWGFDVAAACSGFLYSLTQGAALIESGRYKKVVVCGADKMSAIVDYSDRATCI
                     IFGDGAGAVLLEPNEDGYGILDSILRSDGSGGQYLHMKAGGSLKPASIETVMAKEHFA
                     YQEGQAVFKFAVKGMADVSAELLERNNLAGDDVAWLVPHQANLRIIDATANRMGLPKE
                     K"
BASE COUNT         2535 a         2089 c         1916 g         2928 t
ORIGIN      
        1 ttattcttat gtaccccagt tctctgaatt ccatatgatg aaacctgtac aggtaaataa
       61 acaaaagaag aagaacttat gcggctatgt catgctgttt aaaaggtccg gatctgaaac
      121 cggatcaccg gttccgcaaa atccctgata ataaaaaata taaaattgaa acccatcttc
      181 aaaagagatg ggtttcaatt ttttagacct gataattttg gctcaggttc atattccaat
      241 attgaccgat tggataacac cagccgtttt attatcaata gaatgtttaa tttctgctat
      301 gcattttccg ggctttcgat tggtttcatc gttatgcttt aactacagct gaagttgaga
      361 tacactgctt ttactttgct tccttgtgct cacctgtatt tgtgctctgc cattttctta
      421 aaacaacttt tttattcccc tcccaggttt gcttttcatc tcaacttacg ttctcaacat
      481 cctgctcttc ttactgtgca agcgagatcc ttacctgcaa tccacctctt gtgttagtga
      541 tcgggaaggc actgctgatc ttcggtatat tccggttctg ctcaaagtat aacttcatac
      601 tgatcctgtt attcaggata taatcaattg agggtgcaat acgtattact ttctgtccac
      661 cggttccaaa ggcggttccc tggtccagtt tgctgttagc agttgcatca tcacgaaggc
      721 tgaaatccat ccggaaagta acatcattat ccagtttcat tccgttcttg ccaatcttga
      781 tattctttat aagcgggata ccttttttac gccagttgaa attaaatgtt acttccgttg
      841 agcgattctc cgccaactga tagtcaatta aacttaaact gagtgtacgg ctttttttaa
      901 tttgaaatcc tgttgtcagc tggttagtga atgtgatatc aatttcaatc agcggatcaa
      961 atgattcctg aattgtaata ttgggtacca ggaagtaagg tataaagttt ccggtaagcg
     1021 tatccctgaa agaaggataa cctacccgga aaggatcggc aaataaaagc gcagtattaa
     1081 agctattcat actaaaggtg ctgcgatagc catgacggat ggtaaaattg gtaaatatct
     1141 tatccagtcc tttcattctc gacaaacctg tataggtaat attccagttt ggttttggca
     1201 aaataccgga gaatggattt gccttgatat tggggttgct gttttttgta agctttacac
     1261 ccaacggatc tttattggtg tacgctgcaa ggaaagcagg tatcaccaca tcctgtgcat
     1321 aacgaccata gccttttaca taaccatcag ggtctgtacc gcctgtctga taaggattaa
     1381 ggccaccgag tttgccggat aatattcttc tgttcgcttc aaattgtttg aatgtttcag
     1441 agacaacatt cggatcaaat tttttgaaca aggtctggta agaaatataa ctcacactga
     1501 agctacccag ggcataggga ttcaaccttg taaggccggc acttcccgtt gtgtctttat
     1561 acaattcaga atactgcttg tcaaatgttt tatcaaggtt gatatcaata ttgaaatcac
     1621 ggaacggact tacctgtgcg gtaatattca accgttggtt gtagcgttgc tggatcattg
     1681 cacttaccag ggaatccctt gacaaaagac ctttggcgcc aaaattattt atccagttgg
     1741 tatccggctg gtatccgaaa atataaccga agccgggttc cctgcttttg aaattatttc
     1801 ccaacaactg cgtactgtcc atatatccgg gcagggttgt tccaaagtct tctgtatact
     1861 gaatgcccac tctcttgagc gaagtagcca atcgcaggaa gaatttcgga acattgctta
     1921 tttccggcaa ttgattagga tcctttttgg cttttatctt tcttttctta atccgtatga
     1981 tctttccttt tttattcctg atggtatcaa ttcttattcc cccttctgta ttcacctgtt
     2041 gctgattctt aacctggttt tcattctttg tatcatcacc ggttttccca tcagtatttt
     2101 tattatcccc tttcttttgg ttcttaccac cgccggccgc tttattacct gcgcctgcat
     2161 tgggactact ggtgtttacc gcccgcagga atcgccattt attatacaac tcttcaaatt
     2221 tcaactcgcc attaatagtc cttgtttgag tattggaaag tgtattaccc aggttccttg
     2281 caagcaggga cgatgccaac caattatact ttgtattata attggcacgg agagttgtcc
     2341 agtcaagtaa tggaatttta ttcgtaggta cattatagct gatcgtcaca tcctggctat
     2401 aagttgtatt tcttcctcct ttgaacaaat tctttctaac tgtatctttc ttcaatttag
     2461 tatcaatgcg gccaataggt tcatctatcc gtgcattatt cgtagcggaa aaatcaatcg
     2521 caatagaccg tgtaaggttc cattgtaaaa tatagaaccg gtcgaacgta aagtacttat
     2581 tatacgtctc tggtatctgg tacgggccac cacctacatt acggggtcgg gtggcaccaa
     2641 attgccggaa cacatcagcc cggaatgaaa cctggttggg cgcataattg agattgatgt
     2701 ctttgatgag ggataaccag ggcgatttcg atttgatcag ttttttgaaa ggttcaaagg
     2761 gttttgtttg tggtgcataa gtatacccta tcgctccacg ggtacggcgc atttcatccc
     2821 gctctatcag cgggttatga ctcaatgtct gtatatagga ataattgaaa tcaaaattgg
     2881 taatactcca tggttttggt ttcttaccat ctgttttcaa ttttcttaca ttagttaaat
     2941 tcagtgtttt aatagtagta acatcctgcg catcattctt tattgaatct ctcttatctg
     3001 catcagcatc ctttattttc tgtttcagat caaggtccag atcataagga tcatactcag
     3061 gagtgctggc gcggcggctg atccctgcat agatcgggat ttgcaacccc agctttttag
     3121 gtaataattt tcccgcttca atagttgcag aagcatcaat agtgtacaca tcttccctgc
     3181 tgcgctcatt tactcgctgt tccaacgttc caaagcctct tgacttagct gttcctgaaa
     3241 tagaaatcat accgaggtca gctaatttta tatcggccct ggccaatgcc gcccagccgc
     3301 ctttttcatc caattgctgc aggcgcaatt cattaaacca aacctctgta caaacggatt
     3361 cctgtgtcac attctcaacc cccatcagca tacccctcac ttctcccagg tttggattac
     3421 caagtatcgc ataggacctt ccgttaggca atacttcttt atagtattgt gacggggata
     3481 tgccaagttt atttctcttc gttttcaggt ctgtcagatc atccagcgag aaatcaagat
     3541 tattttcttc cggccagatt ttcaaactat ccgtttctcc aaaatcagtt ttctttaatg
     3601 ggatccgcac ttcataatag ttgccggaaa aatcattacc aatacggact accgcattca
     3661 gatcgccatc ttcaatgggc tgcgcattct gaacagattc tgcatggatg aacatagaca
     3721 gcctgccata ctgccgcata tcaaggttca ttgttttgaa aacaccacgg gattcgaatt
     3781 gtggcaaccc gcaaacctgc atactgatag attgctcatt cagaaacaaa gccacattat
     3841 tattacttaa ctgctgctgt ctttcaatgc cgggaggaat gcgataaaca atgggctgac
     3901 gctgatcgtt ctcttccaca ttcacggcta atacatttac tgtagttgga tcaggagcag
     3961 gcagggtagt ataattcccg gtggtatcaa ttttattttt aaactttctc cactggttgc
     4021 ggatcagttc cagtttacca aaacgcatta ccacagaatc ttcaaaccct gttacgaaca
     4081 tgcggatgaa gcgaatagat ttaaaatccg gaatattccc gattttctgc tggtactgat
     4141 caacagggat gcggaacaaa taccatttct cagtgcggga ttgaccgttg gctaatgtta
     4201 cagtgggtgt tcttacatcg gtaataaaat tagtaccggc agccatgttt ggcctgatat
     4261 caaccttata ctggaaatat tcttccactt cgttcatggt attatccctg ttcagctctt
     4321 ctgcatcggg atattgcgtg aaagcattaa caaactgtgt attgttttcc gcaataggtg
     4381 aattgccatg cgggttattg atattcttat aacgttgcaa gatcccggtc accggttgct
     4441 gatcaaatgc cgggtcacgg tatgctctaa aattatcacc ggaaggatcc gcctgtgcct
     4501 tttgataaat gggtgaattg gttccaaaat ttgactgcag actattcagg tatggtgtaa
     4561 atattctttg ctctgctgta tcctgcaaac catcaaaccc aacatcctgg aactcacgat
     4621 ctgttgctgt attactgaag gcattggtca cctgtaatgg gatggcaggc acttttcccc
     4681 aaacggatga ttcatctacc cttgcattat ttgttggcgt aggcagacca ttttcgtatt
     4741 gtctttttcc atctcttaaa acatcctcag acacattacc caagttgaaa tataactgac
     4801 cgccgtttgg attggctgta ttggcaataa atggatcctg taaccagaat tctatatatt
     4861 ctatattacc cgtttcaaaa tctgtctgat caatgttacg catcatacca ccccatgcct
     4921 gtttgggatt tcttaattta ccattcgcat caaccctgtt cgggtcaaat tcaaaattat
     4981 acggccccag gtcttttgga taaaaagcaa ggtcaaatgt tgtcagcagc ccttctccga
     5041 attgtgtact ccggcgtggg aatatttctt tctgtaaaac ctgtcttgtt tcgggccttg
     5101 ataattcctc cggatcattc gcaagcgggt tattcgaatt tctcctttcc tgtaatacag
     5161 gttcgatatt ataccatgcc agtttccccc ggttataacc acttcttaaa tcattattaa
     5221 gccccgactc cgggaaaagt ccatttccct gcgggatgga agccagcgtc cagcttatca
     5281 gcggaaaccg gagatcaata ccggcacggg taccttcaaa atcatcaatg taaacctgcc
     5341 cgctcctgtc ctgatttccc gaatttattt gccgggcatg cccgggttgt aaaaccgccg
     5401 cttcagcata tgtcgtaata gatgacatgg ccttagtgga atagaacggc aacttatcca
     5461 atagctttgt cagtcgtgga agattattac gatagtcaag atcaaatccg tacatcgtat
     5521 tcttgatggg gtcttcacca tatgattgtt taatgaagaa gggtctttcc cccaatctta
     5581 caattgtacc acccaatgtt aatttattac tggccatgta atcaagacgc aagcccagga
     5641 aattcttttg ttgaaaacca aaagcctggt tattctcata attgatattg accggcactc
     5701 ctgagttaat gattgcctgg ttgaccactc ttagtgttcc taaatcataa ttgatctcgt
     5761 agtcgacatt ctccaccaac acttgtccgc cggcgcttac tgttactgag ccgcggggaa
     5821 tattatatcc taactggtac tcactacctc caccgccact tccaaatccg gaagacttgg
     5881 atttaccaac aattttaaac cggttcaggt tagcataggt ctgtgcaatc gctttgatcg
     5941 tatcgtaaag cggataatat aaatattgat cccgtatggc ctgggatgga tagatataat
     6001 caaggtcccg gccaaagggt tccagtaccg ggaatatgat acggctttgg gaagaaatga
     6061 cggtatatcc ttcaataaag tcaaatactc catctggttg cgggtcgttt tgattattca
     6121 gcctgtccag attaaccagg gaaataatgg gctgtccctt gtaaggatca accacatctg
     6181 ttggaggcag gtatcttttt tcaccaaaac tgggttcttc atacaacatg tccaacatga
     6241 aatcctgtct ttccagttgt ccaaaaccta ctgaataaac attcttcatc atcagttccc
     6301 atatcggaag attagtacgt tgtgaagtgg cctttaataa tttcaggaac aggatacgtt
     6361 gagagtttcc ggtagtatct ggcggcacat cctgcgaaaa ctcacctacc tggaaaacac
     6421 ggccattata agtgtattga aaagctacac ccagcacttc atccggctgt agtggttgat
     6481 tcaaagaaag aaaaccgatc tgcggattaa aataataatc agttggctgc agcttacggg
     6541 caaatgtttt ttcaaaatcc tgcacaggtg ataaaccaaa agtggtcagt tgattttgca
     6601 ccaacgatga attcctgaaa ttaggattag tagtaagcga tgaataaaga ctattcgcat
     6661 cgtttcttgg caacccatca ccagtgcctg tcagaattgg actataagga ttcccttcac
     6721 ccaggtccat aaacgccact atatccctcg tatcagtggt ggaaccatta cggttggtaa
     6781 cccaaacttc cactcttaat acctgtactt tggaattaac gattggcaac tgcttcattg
     6841 ccgtattata gttactgcgg aaatactgag acatgaggaa gtgacggttc tcatcatatt
     6901 catctgcctt cagggaaaac cgctgtgcag cacttcctcc ctgcaatccc attgtttgcc
     6961 gctgtgattg ctgattggcc agcacggtgg tgataaataa tttaccaaac tgtaactgtg
     7021 tttttatacc aaataaggat tgtgcgccag gtatcaatgt tcctttggag gcaaaactgg
     7081 tattacctgc ctggaacatt tttacgatat catcatcctt gccacgaaaa tcaagcttta
     7141 actggttttc aaaattgaaa ttggccagtg tattatagtt gatcgggagt ttcagttttt
     7201 ctcctatctg tgcatccatc tgcaactggg agttcatgtt aaaatcaaat ccgccatttc
     7261 tccgggcccg ttcaggtaat gtaggattct taatattctg accctggtat cctgctagga
     7321 aatcaacata gcctgaaggt ttgatctcta tttttacttt accatccggt ccaactccca
     7381 ttatccggtt gaaccagtca ttagacactt taaattttgg cctgaccatc ctgcggttca
     7441 tattggacat cagcgaggcc ctctttttga aataatcctc ctcatccttt ttcccctgca
     7501 tcctcacaaa ttcttccatg gtgaacgatg ctggcgtacg ataataccgg tttcctattt
     7561 tttctactat atagtattgt tttgttttgg gatcatactc aatggtacgc ttgatgaaag
     7621 cagtatcccg cagatcaaat gtattccggc ttgggttaga ataggggtct ccgtacctgt
     7681 cccgtatcgg gtaacgggtg gtatcatcta aaaacaaatt tctattattg aagaccggtt
     7741 taaaatcacc ggcacttgcc tgtaaacagg aggcaagtac catggtaaaa actaccagtg
     7801 tacggaagat atgagcagtt ctaaggttca atgttttctc gtcaattagt ttcctgaaaa
     7861 tagtggatta gagggtacga agtgccattt taatcagttc ctcaacggat acatttggat
     7921 tggctgcaag agtcttttca atggcctggc tggcagcctg ccggttgatg cctaatgcag
     7981 tcaaagcatt taacgcatcc tgatgcaaag tattgttttt cagtactgaa atatttgatt
     8041 ccagcggatg ttttgccaat ttgtccctga gttccaatac catccgttct gcagtttttt
     8101 tacctattcc tttaatgcct tccagggtct tactatcccc ctgaacgata gccttcgcca
     8161 gttcttcagg cttcatgtat gacaacatca ccctggcggt agatgcgcct ataccggaaa
     8221 cactgagtaa ttgcaaaaac atctcctttt ccgccaggtc aaaaaatccg tagagaatat
     8281 gagcatcttc acggatcagc aagctggtat agagtacccc cttgtcaagg ttttggattt
     8341 tgctataggt attcaggctg atctgtactt cgtagccaac tccggccacc tctacatgca
     8401 ataaagcggg ggttttatgc acaaagtcac ctttcaggaa agctatcatg gctgcgaaaa
     8461 tagggaaatg gttaattggt taatcgggaa tggcagtgtg tattagtatt atccccggaa
     8521 tagctgaata tccggtaaaa tagagacata gccctgcccg ccgattgcct ggtaaactga
     8581 cttcacaatt aattaatcca ccttaacttt ggccactttt atataatatt gagctgcatg
     8641 aataaaaaaa taaccgctgc catcactgct gttggcggat ttgtacctga agacaagctg
     8701 accaatttcg acctcgaaaa aatggtagac accaatgacg aatggatacg tacccgtacc
     8761 ggtatttctg aaagaaggat cctgaaaggt gaagataaag ccacctctga tatggtggta
     8821 ccggctgttc aggagttatg caaaaaaaga ggtatcagtc cggaagagat cgattgcctg
     8881 attgtaggta ctgttacccc ggatatggtt ttcccttcca cggccaatat agcctgcgat
     8941 aaattgggag ctaaaaatgc ctggggcttt gatgtggctg cagcctgttc cggtttttta
     9001 tactcactga cacagggtgc agcgctgatt gaaagcggac gatataaaaa agtggtagta
     9061 tgtggtgctg ataaaatgag tgctatcgtg gattacagtg accgggccac ctgcatcatt
     9121 tttggtgatg gtgccggtgc tgttttactg gagccaaatg aagatggcta tggcatattg
     9181 gatagtattt taagaagcga tggaagtggc ggccaatatc ttcatatgaa agcaggaggt
     9241 tccttaaaac cagccagtat tgaaactgtg atggcaaaag aacattttgc ataccaggaa
     9301 ggacaagccg ttttcaaatt tgcggtaaaa ggaatggcag atgtgagtgc cgaactatta
     9361 gaaagaaata acctggcagg tgatgatgtg gcatggctgg taccccacca ggccaatctt
     9421 cgcatcattg atgctacagc aaacaggatg ggactaccaa aagaaaaa
//
LOCUS       DAORTI010000129         9460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7785_length_9460_cov_7.403190, whole genome shotgun sequence.
ACCESSION   DAORTI010000129 DAORTI010000000
VERSION     DAORTI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_9460_cov_7.403190"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLO70_13520"
     CDS             complement(<1..171)
                     /locus_tag="PLO70_13520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQZ75534.1"
                     /translation="MRAVIQRVSKASVTIDGNVYSQIGNGLLVLMGIEDADTLDDIQW
                     LSSKIVNLRIFDD"
     gene            210..614
                     /gene="arfB"
                     /locus_tag="PLO70_13525"
     CDS             210..614
                     /gene="arfB"
                     /locus_tag="PLO70_13525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQZ75535.1"
                     /translation="MIDLSKEISFQTTRSGGKGGQNVNKVETAVIAFFQVESSALLSA
                     EQKELLKEKLNNRINADGFLHIKAQVHRTQLANKEEAVEKIHELVKKALEKKKARIAT
                     KASKASKEKRIESKKRKADIKAGRQKFRRPDF"
     gene            663..1709
                     /locus_tag="PLO70_13530"
     CDS             663..1709
                     /locus_tag="PLO70_13530"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQZ75536.1"
                     /translation="MRKSLLTIFLLVVTSFTISWLLGACKKDQNTDGIELLQQEIPGS
                     FPQPLYKFEDNPLSKEGFELGRKLFYDPRLSADNLHPCSSCHEQVAAFGTFEHDRSHG
                     VFESHTLRNAPVLFNLAWSSSFHWDGEFVSLQDEAVHPLTGPVEMGETFEGIIKKIQD
                     DPEYRELFSKVFRYPFIRPEFITKALAQFTGSMIAADSKYDRYKKGTVTFNAQEENGY
                     QLYKVNCASCHPEPLFTDYSFRNIGLPLDNLLTDYGRMRITGKSEDSLKFKVPTLRNT
                     YISSNYMHDGRFNTLQQCINHYRTNVQPGPTLDPLLINGISLTNAQATDLVVFLRTLT
                     DSTLLRDPRFSKPN"
     gene            complement(1706..2353)
                     /locus_tag="PLO70_13535"
     CDS             complement(1706..2353)
                     /locus_tag="PLO70_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75537.1"
                     /translation="MKKLLLSFLIVLAVDSILSAQAYEGTVEFDKKKQEAFMIDYPYP
                     PEAVENAITKKMEKLGYKPKEEKGIFNKDKGFRIYKDAFVTEINEERMDYIIKVESRG
                     RKNQGESVVYMILQKDGSNAKTAFDAYQVEKVKTFLNDLQPDVMAENLELDIKAQEEA
                     VGKAEKKLRGLKDDQDDLEKKLKNNKDAQRDTEKEIEAKKQALDELKAKRVIDKT"
     gene            2466..4040
                     /locus_tag="PLO70_13540"
     CDS             2466..4040
                     /locus_tag="PLO70_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQZ75538.1"
                     /translation="MFSYPSGSIAGVSRNKNSNPLSGIKKLAGQTLWYGMSSIFGRFL
                     NYLLTPLLVTIFASGEYGKISTLFTIAAFLNIIYTYGLETSYFRFSALQPESKVYNTC
                     SSSIIVTTVLFTGLLLLSAQSFATFLEMPKHPEYISWVILIVALDTLSVMPFAKLRFD
                     GRPRKFAAIKVLNILINVGLVIFFLVVCKNAYEKNADNLFTTLYDPKIGLGYVILANL
                     IASGITLLLLSKEFFHFNFRLNPGFWKEMMSYSWPLIIVGFGGMINETIDRIMLLKLL
                     PGSTEEAFSQSGIYSANYKLAVLIVLFIQAFRMGAEPFFFKQSTSENAQKTYARVMKF
                     FVIACCFCFLGVTLFLDIWKYFMGTKRPEYYTGLKVVPILMTAKIFLGIYYNLSIWYK
                     IKNKNLTGAWITLGGAAITILFNWIFIPILGYMACAIATILCYGFMMVVSYKLGQKYY
                     PIPYAWKKLVAYIVISLLLFGLHQAFLLFELNHWINRAVAVGFIGLFTLLVLNVERKE
                     FEKMPLIGRILKPKVA"
     gene            complement(4037..4681)
                     /locus_tag="PLO70_13545"
     CDS             complement(4037..4681)
                     /locus_tag="PLO70_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ75539.1"
                     /translation="MLSVKSFTFSPVQENTYVLYNEQKQCCIIDPGCYFPGERDELKT
                     GIENTGLTPVLLLNTHCHLDHVFGNKFVHDTWGLDLHIHQKEKPVLDFAPQSGQMWQL
                     PFDNYDGPLVYLKEGNRIKIGEDELEIRFTPGHSPGSVSFYHEAGGFIIGGDVLFNRS
                     IGRTDLPGGSMEVLIESIQTQFFTLPDDTKVYSGHGPVTTVGFEKMNNPFVKMY"
     gene            4780..8181
                     /locus_tag="PLO70_13550"
     CDS             4780..8181
                     /locus_tag="PLO70_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75540.1"
                     /translation="MKKVLSLIACLSIFASVETAAQVNTVEFGKNRVQYQKFKWKYYQ
                     TENFNSYFSQDGLGLGKYVAQIAEAELPTIEEFVEYGLQRRINIVIYNNYDEMQQSNI
                     GIGIDWQNTGGVTKLVNNKMIVYYDGNHDNLRRQIRQGIARNLVENLLFGDDLGEFAA
                     NQALLDLPKWLTDGYISYAAENWNTTLDDDLRGVMLAGSYRNFYQFAFEKPLLAGHSF
                     WKYIADKYGKSKTTYFLYLARIYRNLNNASYKIAKKKFKVILRDFMTDVQAQYFKDIR
                     GRRNTPRGQLSVSEYIGKKDYIRFNANPVPRSFTYAVVEFNQGRYQVILMENFVNKKV
                     LLKLGVRSREEDKHPNYPILAWDGKGTRLAVLYNEEGKTKFFVYDLVNRVKLWKQEID
                     KFDQVQDMKYMLDNNTLLLSAVKSGQTDIFVYKIDKQTVEQITNDVYDDLDPSFVAFP
                     NKTGIIYSSNRPSANAASSDDSLPGKHFNIYLVDNWNKSDVKQISKLTDLKLGNARFP
                     SQYNTSHFTFISDENGINNRYAGFFKTERAGLDTLVFIGDEMLRNPPQKEVDSLLKEW
                     GKTDIDSVGFVSITNDSSYVFPLTNYQSSMLETRTAGDNQQVSEVVKLGDVKLLYRLK
                     VDENTLRRRNVNARPTEYIRKVIEEEKKAARLEAVQTSKTDSLAKKQQDFFNTGFEEE
                     KPDSTKKVGDVVKADEIIKESVLKKAKVYEYRPPKFFNDYLVSGFNNNVLVTRFQPYQ
                     GGYGPIQLSNNDPLNGIIRVGTSDLFEDWKVAGGFRISPDLTNNEYVFTTQYLKKRID
                     YGITYYRTVVKNPPLYTDSTAFNAKQYSNLYQVTASYPFSKIRSLRMNVGYRSDKYVK
                     LANEFFPEQSLRGKDDRLTYALAHIEFVHDDAITPALNIWNGLRWKIYFDYNAQIGKN
                     NKTGSGDFPYTMNVGFDARHYLPIYRNIIWAVRGAADFSWGTQKFIYFLGGVDNWLMF
                     GQNQRDNGSFRYFDPSNTPDPDAEYAYQSLAVNMRGFKQNVANGNNALVINSEVRVPV
                     FTTFFNKPINNAFLRNFQIVQFVDLGTAWNGTYDKIQRPSVSYTSQPITVRVKAGGVG
                     PFAGGYGFGARSTLLGYFLRFDAAWEMNGVFKGRPMYYFAMGLDF"
     gene            complement(8305..8679)
                     /locus_tag="PLO70_13555"
     CDS             complement(8305..8679)
                     /locus_tag="PLO70_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75541.1"
                     /translation="MVKKNLYLVPLIILIVTILYAVYTVSTTDIVFGNKHYLGILFIA
                     ISVISTIFKKDLGIYFTGITLLIGTLNFIAFTPAIEAYSFGFGLNDKSTTSFKIQMFS
                     FLVLLLYLILNGKFLLSKIIKL"
     gene            complement(8673..>9460)
                     /locus_tag="PLO70_13560"
     CDS             complement(8673..>9460)
                     /locus_tag="PLO70_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75542.1"
                     /translation="NWYGFKWRNHDPQIGRFIEIDPLAEDYVYNSTYAFSENKVTNHI
                     ELEGLEAVSAGNPQGYLVEGFRQIFQAAGNLFSFKAEVHVNKEVEVKTEVKTPVGTVE
                     NKTTTTVVENKVELKTNFGDYFKNGGGKMVEFSAGSNAVSKVENTTTATVNTPGAPLK
                     MFSKTTLDANGRSQTTSVGTGTSVAVGDKGKVTVSAVGFVTQQQTGTNAGQNTFGFKA
                     AVDATFVSKPVIMVNTPAVKVTTNSQTTVGGSVTQTYKLPWQW"
BASE COUNT         2833 a         1933 c         1855 g         2839 t
ORIGIN      
        1 atcatcaaaa atgcggaggt tgacgatctt gctgctgagc cattgaatat catctaaggt
       61 atccgcatct tcaataccca ttaaaaccag caacccattc ccgatctgcg aatacacatt
      121 cccatcaatt gttacactgg cttttgaaac ccgctgaatt actgcccgca ttgtccgtaa
      181 ttttgattta caaatgtaat ggctgaaaaa tgattgacct cagtaaagaa atatcatttc
      241 aaacaacccg aagtggtggt aaaggcggac aaaatgtaaa taaagtggaa actgctgtca
      301 ttgctttctt ccaggtggaa tcatcagcct tactttccgc agaacaaaaa gaattattga
      361 aagaaaaact caacaacagg attaatgctg atggtttttt acatataaaa gcacaggtgc
      421 atcgtaccca actggccaat aaagaagaag cggttgaaaa aatacatgaa ctggtaaaga
      481 aagccctcga aaagaaaaaa gcccgtatcg ccaccaaagc cagtaaagct tcaaaagaga
      541 agcggataga atcaaagaaa agaaaagcgg atattaaagc tggcagacag aagttcaggc
      601 gccccgattt ttaacgcttt gacaaaaagt aaaattgtaa attagctgtt caattgaacc
      661 ctgtgcgaaa aagcctgctg acaatatttt tattagttgt tacatctttc actatctcct
      721 ggctgttggg tgcctgtaaa aaagatcaga atactgatgg tattgaatta ttacaacagg
      781 aaattccggg tagctttcca cagcctttgt ataaatttga agacaaccca ctaagtaaag
      841 aaggttttga actgggccgg aaattatttt acgatccccg gctttcagca gacaatctgc
      901 atccctgctc ttcctgtcat gaacaggttg cggctttcgg cacatttgaa catgatcgca
      961 gccacggcgt ttttgaatca catactttac gaaatgcacc ggtattattt aacctggcat
     1021 ggagctcttc ttttcattgg gatggggaat ttgtttcctt acaagatgaa gcggttcacc
     1081 ctcttaccgg gccggttgaa atgggagaaa catttgaagg tatcattaaa aaaatacaag
     1141 atgatccaga atacagggaa ctgttcagca aagtattccg ttacccgttt atcaggccgg
     1201 agtttatcac caaagcactg gctcagttta ccggttctat gattgctgct gattctaaat
     1261 atgaccgtta taaaaaagga acggtcactt ttaatgcaca ggaagaaaac ggttaccagt
     1321 tatacaaagt caattgtgcc agttgccacc cggaaccact atttactgat tatagcttcc
     1381 ggaatatcgg gttaccgctc gataatttac tcacagatta tggacggatg aggatcaccg
     1441 ggaaaagcga agactcacta aagtttaaag tacctacgct gcgcaacact tatatttctt
     1501 caaattatat gcatgacggg aggttcaata ccctgcagca atgtattaat cattaccgca
     1561 caaatgtaca gccgggccct acccttgatc ctttactgat aaacgggatt tcattgacaa
     1621 atgcgcaggc tactgacctt gttgtatttt tgcgaacact gactgattca acattattaa
     1681 gagacccccg tttttctaaa ccgaattagg tcttatcaat gacccttttc gccttcagct
     1741 catcaagtgc ctgtttcttt gcttcaattt ctttttctgt atcccgctgt gcatctttgt
     1801 tattctttag tttcttttcc aggtcatcct ggtcatcttt caaccctctg agtttctttt
     1861 cagctttacc aaccgcttct tcctgtgctt ttatgtccag ctccaggttt tcggccatca
     1921 catccggttg aagatcattg aggaatgtct taactttttc aacctggtac gcatcaaaag
     1981 cagtttttgc gttactccca tctttctgca ggatcatgta tactacagat tcaccctggt
     2041 tttttctgcc cctggactca actttaataa tataatccat cctctcctca ttgatctctg
     2101 taacaaatgc gtctttatat atcctgaatc ctttgtcctt attaaaaatg cctttttcct
     2161 cttttggttt ataacccagc ttctccattt ttttggtaat tgcattttcc accgcttccg
     2221 gcgggtaggg gtaatcgatc ataaaagctt cctgcttttt tttatcaaat tccacagtcc
     2281 cctcataggc ttgtgccgaa agaatactat caacagctaa aacaattaaa aaggataaga
     2341 gtaacttctt catatcaagt gattttgagg ataaagatac agcaaggctc aaaagcgttg
     2401 caattgtctt aaataaaata tctacctggg ccggaattcg ttttttgtca ggctaacaac
     2461 tacttttgtt cagctatccc tccggatcaa ttgccggagt aagcagaaac aaaaattcca
     2521 atcctttgag tggtattaaa aagctggcag ggcaaacatt gtggtatgga atgagcagta
     2581 ttttcggcag gtttctcaat tacctgctga caccactgct ggttactatt ttcgcatccg
     2641 gcgaatacgg taaaataagt acccttttta ccatcgctgc ttttcttaat attatctaca
     2701 cctacggact ggaaacatct tatttccgtt tttcagcact gcagcctgaa tcaaaagttt
     2761 acaatacctg ttcttcatct atcatagtta caactgttct attcacgggt cttttgcttt
     2821 tgtctgcaca aagttttgct acattcctgg aaatgcccaa acacccggaa tatatttcct
     2881 gggtcattct tattgtggca ctggacaccc tcagcgtaat gccatttgcc aaactaagat
     2941 ttgacggaag gcccagaaaa tttgccgcga taaaagttct gaacatcctc atcaatgtgg
     3001 gattggtgat tttctttttg gtggtttgta aaaatgcata cgaaaaaaat gcagacaacc
     3061 tgtttactac attatatgat ccaaaaattg gccttgggta tgtaattctt gccaacctga
     3121 tcgcaagcgg tatcacttta ttattactga gcaaggaatt ctttcatttc aatttccgtt
     3181 taaatcccgg tttctggaaa gagatgatga gttacagctg gccgctgatt attgttggtt
     3241 tcggtggcat gatcaatgaa acaattgatc gtatcatgtt attgaaattg ttgcccgggt
     3301 ctacagaaga agcattttcc caatctggta tttattctgc caactacaaa ctggcggtat
     3361 tgatcgtatt attcatacag gcattccgta tgggagcaga accctttttc tttaaacaat
     3421 ccaccagtga aaacgcacaa aaaacctatg caagggtgat gaaatttttt gtgattgcct
     3481 gctgtttttg ttttcttggc gtaaccttgt ttctggatat atggaaatac tttatgggaa
     3541 ccaaacgtcc ggaatattac accgggctga aagtagtgcc catactgatg actgctaaaa
     3601 tatttctcgg catttattac aatttgtcta tctggtataa aataaagaac aagaacctga
     3661 ccggtgcctg gatcactttg ggtggagcag ccattaccat cttattcaac tggatattta
     3721 ttcccattct cggttatatg gcctgcgcca ttgccaccat tctttgttac ggttttatga
     3781 tggtggtaag ttataagttg ggtcaaaaat attatcccat accatatgcc tggaaaaaac
     3841 tggtggctta cattgttata agtttacttc tgtttggact gcaccaggca tttttacttt
     3901 tcgaactgaa ccattggata aaccgggctg tggccgttgg tttcatcggc ctgtttacac
     3961 tattagtatt gaatgtagag cggaaggagt ttgaaaaaat gccgttgata ggcaggatat
     4021 tgaaaccaaa agttgcttaa tacatcttca caaaagggtt attcattttc tcaaatccta
     4081 ctgtagttac cggcccatga ccggaataca ctttggtgtc atccggcagg gtaaaaaact
     4141 gggtctggat actttcaatc aatacctcca tactgccccc cggcaggtcg gtacgaccaa
     4201 tactgcggtt aaacaataca tcacccccta taataaagcc ccctgcttca tgataaaagg
     4261 atacacttcc gggtgagtgg ccgggagtaa aacggatctc cagttcatct tcccctattt
     4321 taattcgatt tccttccttt aagtaaacca acgggccgtc ataattgtca aaaggaagtt
     4381 gccacatctg gcccgattgt ggagcaaaat ccaggaccgg tttttccttt tgatgtatat
     4441 gaaggtcgag cccccaggta tcatgaacga atttgttacc aaacacatga tccaggtggc
     4501 agtgggtatt tagcagcaaa acaggggtaa gaccggtatt ttcaatgccc gtttttaatt
     4561 cgtccctttc tccgggaaaa taacaaccgg ggtcaataat gcaacactgc ttttgttcgt
     4621 tatacaaaac ataagtattc tcctgaacgg ggctaaaagt gaatgactta acacttaaca
     4681 tggatgggtt ttcgtacttt tagttttaaa ttgggaagta attgtaaaga taatgccgcc
     4741 tgctcttaac gggcatttta ccggcagaaa aaagagatta tgaagaaagt attaagcctg
     4801 attgcctgtc tttccatttt cgcctctgtg gaaactgcag cacaagtgaa caccgtggaa
     4861 ttcggaaaga accgggttca ataccagaaa ttcaaatgga aatattacca gactgaaaat
     4921 ttcaatagct atttcagcca ggatgggttg ggactgggta aatatgtggc ccagatcgct
     4981 gaggcagaat taccaactat tgaggaattt gtggaatatg gattacaacg ccgcatcaat
     5041 attgttatct acaataatta tgatgaaatg cagcagtcca atatcggtat cggtattgac
     5101 tggcagaata ccggtggagt taccaaattg gtgaacaaca aaatgatagt gtattatgat
     5161 ggcaatcatg ataatctccg ccgccagata agacaaggta ttgcccgcaa cctcgtagag
     5221 aatcttctct ttggtgatga tctcggagaa tttgcagcta accaggccct gctcgatctt
     5281 cccaaatggc tgaccgatgg atatatctcc tacgctgctg aaaactggaa caccacgctg
     5341 gatgatgacc ttcggggagt aatgctggca ggtagttatc gcaatttcta ccagtttgct
     5401 tttgaaaaac cattgttggc cggtcattca ttctggaaat atattgctga taaatatggc
     5461 aagagtaaga ccacttattt tttgtacctg gcccgtatct atcgtaacct gaataatgct
     5521 tcttataaaa ttgcaaaaaa gaaattcaaa gtaatcctgc gggattttat gacggatgta
     5581 caggcacagt atttcaaaga tatccgtggc cgtcgcaata ccccgagagg acagctttct
     5641 gtaagtgaat atataggcaa gaaagattat atccgtttta atgcaaaccc ggtgccccgc
     5701 agctttactt atgcagtagt tgaatttaac cagggccgct accaggtaat actgatggaa
     5761 aattttgtca acaaaaaagt attactgaaa ctgggtgtac gttcaagaga agaagataaa
     5821 catcctaatt atcctattct tgcctgggat ggtaagggta caagattagc tgtattatat
     5881 aatgaagaag gaaaaaccaa attctttgtg tatgacctgg tgaacagggt taaactctgg
     5941 aaacaagaaa tcgacaaatt tgaccaggtg caggatatga agtatatgct ggacaacaat
     6001 actttattac taagtgctgt aaaaagtggt caaacagata tattcgttta caaaatagat
     6061 aagcaaaccg tagaacagat caccaatgat gtgtacgatg acctggatcc ttcctttgta
     6121 gctttcccga ataaaacagg tatcatttac tccagtaacc gtccgtcggc taatgcagcc
     6181 agcagtgatg attcattacc cggcaaacat tttaatatat acctcgttga taactggaat
     6241 aaatctgatg ttaaacaaat ttcaaaactt accgatctca aattaggcaa tgcaagattt
     6301 ccttcacaat acaatacttc tcactttact ttcataagcg atgagaacgg tatcaataac
     6361 cgctatgccg gattctttaa aacagaaagg gctggtttgg atacattggt ttttatcggc
     6421 gatgaaatgc tccgcaaccc accacagaaa gaggtggaca gcttgctgaa agaatggggc
     6481 aaaaccgata ttgattctgt gggctttgtt tctattacca atgattcctc ttatgtgttt
     6541 cccctgacca attaccagag cagtatgctg gaaacccgta ctgccggtga taaccagcag
     6601 gtaagtgaag tagtgaagct tggtgatgtt aaattgctct accgtctgaa agtggatgaa
     6661 aatacactac gtcgccgcaa tgtaaacgca agaccaaccg aatacattag aaaagtaatt
     6721 gaagaagaaa agaaagctgc ccgcctggaa gctgtgcaaa cgtctaaaac agatagcctg
     6781 gctaaaaaac aacaagattt tttcaacacc ggctttgaag aggagaaacc tgatagtacc
     6841 aaaaaagtag gcgatgtggt aaaagcagat gaaataataa aagaatctgt attgaaaaaa
     6901 gcaaaagtat atgaataccg gccacctaaa ttttttaatg attatctcgt aagcggtttc
     6961 aataataatg tactggtcac ccgttttcag ccttaccagg gcgggtatgg tcccatccag
     7021 ctcagtaata acgatccgct gaatggtatc atccgggtag gaacatccga cctgtttgaa
     7081 gactggaaag tggctggtgg cttccgtatc agtcctgacc ttacaaataa tgaatatgta
     7141 ttcaccacgc aatacctgaa gaaaaggata gactatggga tcacttatta ccgtacggta
     7201 gtaaaaaatc ccccgttata tacagattct actgctttca atgcaaaaca gtattcgaat
     7261 ctttaccagg ttacagcaag ctaccctttc agcaaaatca gaagtctgcg catgaatgtt
     7321 ggctatcgca gtgataaata tgtaaaactg gcaaatgaat ttttccctga acaatcctta
     7381 cggggaaaag atgacaggtt aacctatgca ttggcgcata tagaatttgt gcatgatgat
     7441 gccatcaccc cggcactcaa tatctggaac ggtctacgct ggaaaatata ctttgattat
     7501 aatgcacaga tcggaaaaaa taataaaacc gggagcggtg acttccccta cacgatgaat
     7561 gtgggatttg atgcccgcca ttacctgcct atttatcgaa atattatctg ggctgttcgg
     7621 ggtgctgctg atttttcctg gggtacccaa aaattcatct acttcctcgg tggtgttgat
     7681 aactggctga tgtttggaca gaaccagcgg gataatggtt ccttccgcta ttttgatcca
     7741 tccaacaccc ctgacccgga tgctgaatat gcttatcaat cattggcagt gaacatgaga
     7801 ggctttaagc agaatgttgc caatggcaat aatgctctgg taataaatag cgaagtaagg
     7861 gttcctgtat ttaccacttt cttcaataaa cctattaaca atgctttcct ccgcaatttc
     7921 cagatagttc agtttgtgga tctgggtacg gcatggaatg gcacctatga taaaatacaa
     7981 agaccttctg tttcttatac ctctcaaccg ataacggtaa gggtaaaagc aggtggtgtg
     8041 ggtcctttcg caggtggtta tggctttgga gccaggagta cattgctcgg atatttcctg
     8101 cgctttgatg cagcctggga aatgaacggt gtgtttaaag ggaggccgat gtattacttt
     8161 gcgatgggat tggatttttg atcaaattta ttcttcaata caaagccctc tcacagtacc
     8221 gggattcttt agcttttatt atttgcagcc catcgttgca gatgatggac aaccgctcaa
     8281 cattctactg aaattggggg ataattaaag tttaattatt ttactaagta aaaattttcc
     8341 atttaatatc aggtataaga gcaataccaa aaatgaaaac atctgtattt taaaactcgt
     8401 tgtgctttta tcattcaacc caaacccaaa agaataggct tcgattgcag gagtaaaagc
     8461 aataaaattt aatgtaccaa tcaaaagtgt tatacctgta aaataaattc ctaaatcttt
     8521 tttaaaaata gtacttataa cagatattgc aataaacaaa attcctaagt aatgcttatt
     8581 accgaataca atatctgtag ttgatacagt atatacagca tacaaaattg ttactatcaa
     8641 tattattaaa ggaactaaat aaagattctt ttttaccatt gccatgggag tttatatgtt
     8701 tgagtaacgc ttccgccgac tgtcgtttga ctgttagtag ttacttttac tgctggtgta
     8761 ttaaccataa taacaggctt acttacaaat gttgcatcaa cggcagcttt aaaaccaaaa
     8821 gtattttgcc ctgcattagt tcctgtttgt tgttgtgtaa caaaccctac agcagataca
     8881 gtaaccttac ctttatcgcc aacggctact gaagttcctg tgccaactga agttgtttga
     8941 cttcttccat ttgcatccaa agtagttttc gagaacattt taagaggtgc tcctggtgtg
     9001 tttactgtgg cagttgtggt attttcaact ttagaaacgg cattgcttcc tgctgaaaat
     9061 tcaaccatct ttccaccacc atttttaaag tagtcgccaa aattggtctt caactccacc
     9121 ttgttttcga ctacagttgt tgttgtttta ttttcaactg taccgactgg agttttaact
     9181 tctgttttaa cttccacctc tttattaaca tgaacttctg ctttaaatga aaaaaggtta
     9241 cctgctgctt ggaatatttg cctaaagccc tccactaaat agccttgagg gttaccggca
     9301 gatacagctt ctaatccttc aagttctata tgattcgtta ctttgttttc agaaaaggca
     9361 tatgtagaat tataaacgta atcttcagcc agtgggtcta tttcgataaa ccttccaatt
     9421 tgtgggtcat gattccgcca cttaaatcca taccaattta
//
LOCUS       DAORTI010000130         9308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_7986_length_9308_cov_6.067654, whole genome shotgun sequence.
ACCESSION   DAORTI010000130 DAORTI010000000
VERSION     DAORTI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7986_length_9308_cov_6.067654"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..597
                     /locus_tag="PLO70_13565"
     CDS             151..597
                     /locus_tag="PLO70_13565"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HQZ75543.1"
                     /translation="MQHRILQLITGFIFLSLFSSFRPVAEEGIKINVTNLRSNTGNVL
                     VSLFKDGVGYPDDATKAFRKTKLSITDKKASILFSNLPSGVYTVSILHDENDDQKMNK
                     NGLGIPKEGYGFSNNVMGAFGPPSLSKTSFKHTATSLTQKDIRVRY"
     gene            complement(648..1064)
                     /locus_tag="PLO70_13570"
     CDS             complement(648..1064)
                     /locus_tag="PLO70_13570"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQZ75544.1"
                     /translation="MSNRTFTMIKPDAFKNGHAGAIIDRIIKEGFSIKAMKMLKLSPE
                     KAGEFYAVHKERPFYGELVEFMSSGPIVAAILEKENAVEDFRKLIGATNPANAEEGTI
                     RKLYATSVGENAVHGSDSDENAKIEGDFMFSGLEQF"
     gene            1252..2259
                     /locus_tag="PLO70_13575"
     CDS             1252..2259
                     /locus_tag="PLO70_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQZ75545.1"
                     /translation="MKPIQDIFPLLSQPRRVVITTHQKPDADAMGSSLAMRHFLSQFG
                     HNVSIISPTNWAGWVNWMPGTKEVLDYEKDKQKAEAILDNAEWLFCLDFNIFHRTKTM
                     TEKLKQLTCTKILIDHHQEPDVDSFDYGISNTAKSSTCEMVYDFIVDSGHGDKINEEI
                     GECIYAGVVGDTGSFRFSSAHAGVHSLVADLKSRGLNHGKIHEGLFDNFLENRLRFIG
                     HVLLHRMEIFYEFNTALIAIPKSDILKYHIKTGDTEGLVNFPQSIQGIKLVALVIDRD
                     EERKWSFRSKGEFDCNTFARKYFEGGGHYNAAGGRSSDSLEQTVEKFKLAIKENESLL
                     H"
     gene            2272..3198
                     /locus_tag="PLO70_13580"
     CDS             2272..3198
                     /locus_tag="PLO70_13580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQZ75546.1"
                     /translation="MNTIKNLLLIVTVGALAASCSKATYRKTPGGMPYQLFASGDTQQ
                     VQVGNFIKVSITQKINDSVYFSTAGKLPVYMPVIAQSSPYDISEVWTKLHLGDSVVST
                     QMMDTFIKRMPPGQIPPQFKKGDRILTYVKILGIFTNDSAKSVDEEKVSTAFAQNEIQ
                     AVEAFVKSKNISTVKTPSGAFVEIINPGTGNLIDSGKYVSVNYTGTTFEGKKFDSNTD
                     SAFNHVQPLSFTVGTGQMIKGFDEAMKFLKPGGSAKVYMPSMLGYGAAPPPGSTVIKP
                     YENLIFEFTVTDVKDKAPETANTPGQPEQPHQ"
     gene            complement(3498..4328)
                     /gene="folP"
                     /locus_tag="PLO70_13585"
     CDS             complement(3498..4328)
                     /gene="folP"
                     /locus_tag="PLO70_13585"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQZ75547.1"
                     /translation="MFTLNCKGRLLVVDKPLVMGIINVTPDSFYRESRHPTADAVLRQ
                     AEQMLHDGAGILDIGGQSTRPGSEKLSADEELKRVIEPVAAIHKRFPEAFISIDTYYS
                     EVAAGAIAAGADIVNDISAGALDKRMIETVAALNVPYVLMHMKGTPQDMQQQAVYENV
                     TREVLDFLINKISESRKAGIKDIIADPGFGFAKTTDHNFELLRNLSLFNIPGCPVLLG
                     ISRKSFIYKTLGTTAANALNGTTALNMMGLMNGASILRVHDVKEAVETIKLFSACKLS
                     "
     gene            4472..5002
                     /locus_tag="PLO70_13590"
     CDS             4472..5002
                     /locus_tag="PLO70_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HQZ75548.1"
                     /translation="MTTTNQQYDKAIASCRDIFLKKAKDYGTAWRVLRTISIVDQIFI
                     KAQRIRTIQEKGEQKVSDGIAAEFKGIINYAVIGLIQLELPASAPEEMNIDEVSILYD
                     KYINTARQLMQDKNHDYGEAWRSMSQESFVDLILMKLQRIRQILTNEGKTIISEGIDA
                     NYHDIINYAVFALILM"
     gene            5004..6191
                     /locus_tag="PLO70_13595"
     CDS             5004..6191
                     /locus_tag="PLO70_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQZ75549.1"
                     /translation="MKVMVTIVRIIVGVLFIFSGLVKANDPLGLSYKMQEFFEVWGLH
                     GFNSWTLLMSVLMNAFEIIAGFALLLGWRIRLFSWLLLLLIVFFTFLTGYTYITGMPK
                     NCGCFGDCLPITSQTSFLKDVALTLLIGFLIWKQRYINLLFSEKPATIVMLGITILSF
                     GFQWYTLTYLPVVDCLPFKKGNNITEQMKMPANAVPDSTVITFVYKKEGKEVEFTADK
                     FPADFSATTYEFVNRYDKVIRKGKNNEPPIKGFALSGESNTDSTRFVLEQESAILLFC
                     EKFSDAGTDWKKKFEEIYTAARAKNIASFIITAEPGKAKDALAGTRFADVPVFKCDFK
                     VIETAARSNPCIYFIKKGTVISKYSGKEMNNIKKNISVATAQVRWPLTPAEQSADSTI
                     QQP"
     gene            6188..7252
                     /locus_tag="PLO70_13600"
     CDS             6188..7252
                     /locus_tag="PLO70_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ75550.1"
                     /translation="MIKFVTKKIVYGLLVLVGVVILVFFLFQGFGDPSRIVMGQTGDS
                     TTQSNIRKELYLINEKGESIPKFRQFLYYLNDVSPICFHSTADINKKKLRGFFIGEGK
                     KFGIKIPYLRKSYQSKREVGTILMQALPGTLMLAITAMFIAVLIGIPLGVMAAVKQNT
                     WLDTTAIFSSIVGISAPSFFMGIIMVYLFGFVLSDWTGLHIAGSWFAIDDEGQRRLTM
                     QNLILPAITLGIRPLAIITQLTRSAMLDVLDQDYIRTAYAKGLSKRTVIWKHGLRNAL
                     NPVITAITGWFAELLAGAFFVEYIFGWQGIGKVTVDALEKLDYPVVMGSVLVAATFFI
                     IINILADILYGVVDPRVRVE"
     gene            7404..7811
                     /locus_tag="PLO70_13605"
     CDS             7404..7811
                     /locus_tag="PLO70_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQZ75551.1"
                     /translation="MEKVTSILERKQFHFKKISPSCSISDALCRMSSHNTDYLIVMDD
                     NDNFLGLLTEHDVASKTIFLKQPLAKASVNEMMNTRLPFADVDDTVEECMKAMKRYHV
                     RYLPVFKNLVLMGIVSTDDILDEAVSHRNEIFD"
     gene            complement(7844..8125)
                     /locus_tag="PLO70_13610"
     CDS             complement(7844..8125)
                     /locus_tag="PLO70_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQZ75552.1"
                     /translation="MGEYSISEAMQQFLNGSRIKGGIQALQIEDVWEEIMGKTISRYT
                     DKLQIIGDKLIITTNVAPLKNELIYQKEKIKQRVNEALKQKVINEVIIQ"
     gene            complement(8135..9217)
                     /gene="recF"
                     /locus_tag="PLO70_13615"
     CDS             complement(8135..9217)
                     /gene="recF"
                     /locus_tag="PLO70_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218293.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQZ75553.1"
                     /translation="MLLLQHISLYQFKNYTNKSFDFTERIAGICGKNGVGKTNLLDAI
                     HYLCFTKSYFTRLDGNNVKHGSAGFRIEGKLELNGKPEKTVCILRETGKKEFSVNDEQ
                     YEKLSQHIGRYPCVVIAPDDAELITGDSKERRTFLDALLSQLDTAYLQNLIIYMKVLQ
                     QRNSVLKNFAETNSRDFTLLEVLDGQLIKPGEYIFEKRKEFLVSFLPAVKHLYNDIAR
                     QPENVTLIYESELLLASFAELLSVNRQKDCLAQRTTSGIHRDDLDIQLGNQLFKNIAS
                     QGQRKSLLFALKLAEMDVLKKEKGFAPLLLLDDVFEKLDEDRISNLLNKVCVENDGQV
                     FITDTNEDRLIAHLKELNTNFQLITL"
BASE COUNT         2716 a         1896 c         1915 g         2781 t
ORIGIN      
        1 aataaagtga aaaaggtgaa ggagatttaa aattagctgg tacagatttt actttatttt
       61 caaatgaaaa ctgtactatg agttgcctat gtttatggtt tgtcctctga aaccatacaa
      121 ccattttcct ttttaattgt tctaaaggat atgcaacata gaattttaca acttattact
      181 ggctttatct tcctgtcctt attcagttcc ttcagacctg ttgcggaaga ggggataaag
      241 atcaatgtta ccaatcttag aagcaatact gggaatgtcc tggtcagcct gttcaaagat
      301 ggggtaggtt acccggatga tgccacaaag gccttcagaa aaacaaagct ttcaattact
      361 gataaaaaag catccatatt attttccaac ctgccttccg gtgtctatac tgtttctatc
      421 ctgcatgatg agaacgatga ccagaaaatg aataagaatg ggctgggtat tcccaaagaa
      481 ggctatggtt tttccaataa tgtgatggga gcttttggtc cgccatcatt gtcaaaaaca
      541 agttttaaac atacggccac ctcacttact caaaaagata tccgggtcag gtattaaaaa
      601 gccgcatcgt ttaaaacgac acggctttga attattcaaa taatttatca gaattgttcc
      661 aatccggaga acatgaaatc tccttcaatc tttgcatttt catcgctgtc acttccgtga
      721 actgcattct caccaactga agtagcatat aacttacgaa ttgtaccttc ttcagcattt
      781 gctgggttgg tggcaccaat cagcttgcgg aaatcttcta cagcattttc tttttccagg
      841 attgccgcca cgatagggcc gctgctcatg aactctacca gttcaccata aaagggtctt
      901 tctttatgta cagcgtagaa ctctccggct ttttcaggag acagtttcag catcttcatc
      961 gcttttatgc tgaatccctc tttaatgata cggtcaataa tggcaccggc atgcccgttt
     1021 ttaaatgcat ccggcttgat catggtgaat gtacgattgc tcatagttac gttatttgtt
     1081 aattgttttt tgcgccgcaa aggtaagtga aaggcaggag tctgtagccg agaacacgga
     1141 gtttttctta gcctatattg aagtcctcct tattctccct acgattgact caagactccc
     1201 ttctgaatac aacttactct gcacacattt ccctaccttc gcagccgctt tatgaagcct
     1261 atacaggaca tatttccctt attgagtcag ccgcgaagag tagtgatcac cacccatcag
     1321 aaaccggatg cagatgcgat gggttcttcg ctggccatgc gccactttct aagccagttt
     1381 ggccacaacg tttccatcat ttctcccact aactgggccg gttgggtaaa ctggatgccg
     1441 ggtaccaaag aagtgctgga ctatgaaaaa gataagcaaa aggctgaagc tattctggat
     1501 aatgccgaat ggttattttg tcttgacttc aatatctttc atcgcaccaa aacgatgact
     1561 gaaaagttga aacagcttac ctgtactaaa attttaattg accatcacca ggaacctgat
     1621 gttgattctt ttgactatgg catcagtaat acagcaaaaa gctccacctg tgaaatggtg
     1681 tatgatttca ttgtggactc aggtcatggt gacaagataa acgaagagat aggcgaatgc
     1741 atctatgccg gggtggtggg tgacaccggt tctttccgct tttcctctgc acatgccggg
     1801 gtgcattcat tggtggctga tttaaaatca agaggactga atcatggcaa aatacacgaa
     1861 ggccttttcg ataatttcct ggaaaacagg ctccgtttca ttggtcatgt attgttgcac
     1921 cggatggaaa ttttctacga gtttaacact gcgctgatcg ctattcctaa aagtgatatt
     1981 ctgaaatatc atatcaagac aggagataca gaagggctgg tcaattttcc acagtccata
     2041 cagggaataa aattagtggc cctggttatt gacagggatg aggaaagaaa atggagcttc
     2101 cgcagcaaag gagagtttga ctgcaatacc tttgcccgca aatattttga aggcggcgga
     2161 cattacaatg cggctggcgg ccgcagcagt gattcgctgg agcagacagt agagaaattt
     2221 aaactggcca taaaagaaaa cgaaagtctt ttacattaaa taaaaacaag tatgaacaca
     2281 attaagaatt tgttactcat tgttactgtt ggtgcattag cagccagctg ttcaaaagct
     2341 acttaccgta aaacaccggg aggtatgcct taccagttat ttgccagcgg cgatacacag
     2401 caggttcaag taggtaactt tataaaagta agtatcactc aaaaaataaa cgacagcgtt
     2461 tatttttcaa ctgccggaaa attgccggtt tatatgcctg tcattgcaca gtccagcccc
     2521 tacgatattt ctgaagtgtg gacaaagctg cacctgggtg acagtgttgt gtccacacag
     2581 atgatggaca cttttatcaa acgtatgcct ccgggacaga tcccgccaca gtttaaaaaa
     2641 ggtgacagga tacttactta tgttaagata ttaggcattt ttaccaacga cagtgccaag
     2701 tctgttgatg aagaaaaagt aagtacagca tttgcccaaa atgaaattca ggcagtggag
     2761 gcttttgtaa agtcaaaaaa tatcagtact gtcaaaactc catccggggc ctttgtagag
     2821 atcattaatc ccggtaccgg taatctaata gattccggga aatatgtatc tgtaaattac
     2881 accggtacaa cctttgaagg gaaaaaattt gacagtaaca cagactcagc atttaatcat
     2941 gtgcagccgc tttcatttac tgtcggtacg ggtcaaatga taaaaggatt tgatgaagca
     3001 atgaaattcc tgaagcccgg tggttcagct aaagtataca tgccttccat gctgggatat
     3061 ggtgctgcgc cacctccggg atcaactgtg atcaagccgt atgagaacct gatatttgaa
     3121 tttacagtta cagatgtaaa agataaggca cctgaaactg caaatacacc aggtcagcca
     3181 gagcagcccc atcaataaaa attgatacag ctcaaccaaa aaaacaagtt tgtattaaat
     3241 aatactgcac ctttgtaccc gtctacgtca gacgggtatt tttttgagag gccccgtttt
     3301 tagtccagga tctatttcaa acccgtccgc accccctgaa attactaacc tgagcacttt
     3361 attacaccgt actttttttg cttgaaccaa tagttaatat cctttgaagc aaacctgaaa
     3421 atccagtgta acaaggtttt gattacaatg tcttaatgaa aaaaaccctt tcacttctgt
     3481 gaaggggttc tttattttca ggaaagcttg caggctgaaa acagttttat tgtttcaaca
     3541 gcttccttaa catcatgcac tctcagtatg gaagcaccgt tcatgagtcc catcatattc
     3601 agggcagtag taccgttcaa ggcatttgct gctgtcgtgc caagtgtttt atatataaat
     3661 gattttcggg agatgcccaa taataccggg cagccgggta tattaaagag agacaggtta
     3721 cgaagcagtt caaaattatg atcggttgtt tttgcaaaac caaaacccgg atcggcaata
     3781 atatctttta ttcctgcctt tcttgattca cttattttat taattaaaaa gtcaagcact
     3841 tcacgggtga cattttcata cacggcttgt tgctgcatat cctgcggtgt acctttcatg
     3901 tgcatcagaa catatggaac attcaatgct gctaccgttt caatcattct tttatccagg
     3961 gcaccggcac tgatatcatt gacaatgtct gcaccagccg caatggcccc ggctgccact
     4021 tcggaataat aagtatcaat ggagataaat gcttccggga atcttttatg tatagctgca
     4081 accggttcaa taaccctttt caattcttca tccgcactca gtttctcact gccgggtctt
     4141 gtactttgac cacctatgtc gagaatacca gcaccatcat gtagcatttg ttcagcctgg
     4201 cgcaaaacag catctgccgt tggatgccgg ctctccctgt aaaatgaatc aggtgtgacg
     4261 ttgatgattc ccatcaccag gggcttatcc acaaccaata atcttccttt gcaattaagc
     4321 gtaaacatta gcctttgttc attatttttg ataaagttag cctggaagac tgaaagtccg
     4381 gatgaatatt cacaactcaa tattgagttt tgctgcatga atgctgcttt ctgactcccg
     4441 gacttccgta cattcttact ataccaaaca catgaccact acaaaccagc aatacgacaa
     4501 agccatcgca tcctgcaggg atattttttt aaaaaaagca aaggattacg gaactgcatg
     4561 gagagtgctg agaacgatct ccatcgttga ccagatattc atcaaagccc agcgtatccg
     4621 caccatacag gaaaaaggtg agcaaaaggt atcggatggg atagcagcag aattcaaagg
     4681 catcatcaat tatgctgtga ttggtttgat tcaattagaa ctgccggcca gtgcccctga
     4741 agaaatgaat attgacgaag tatccatttt atatgataag tatatcaata cagccaggca
     4801 actgatgcag gataagaacc acgattatgg cgaagcctgg cgcagtatga gccaggagag
     4861 ttttgttgac ctgatcctga tgaaactgca acgtatccga cagatattaa ccaatgaagg
     4921 aaaaaccatc atcagcgaag gtattgatgc caactaccat gatatcatca actatgcagt
     4981 gtttgcgtta atactcatgt aatatgaaag taatggtgac catagtacga attatcgttg
     5041 gggtgttatt tatattctcc ggcctggtaa aagctaacga cccgctggga ctgagttata
     5101 aaatgcagga attttttgaa gtctgggggc tgcatggttt caattcatgg acattattaa
     5161 tgtctgtatt gatgaatgcc tttgagatca ttgcaggatt tgctttgctg ctcggctgga
     5221 ggatcaggtt gttcagttgg ttattattat tgctgatcgt tttctttaca ttccttaccg
     5281 ggtatactta cattacgggt atgcccaaaa attgcggttg ttttggtgat tgcctgccga
     5341 taacgtccca aacatctttc ttaaaagatg tggcattgac cctactgatc ggtttcctga
     5401 tctggaaaca gagatacatc aatctccttt tttctgagaa gccggccact atcgttatgc
     5461 tggggatcac catactaagt tttggttttc aatggtacac cctgacttat ttgccggtgg
     5521 tggattgttt accttttaag aaaggcaaca atattacaga gcaaatgaaa atgcctgcca
     5581 atgctgtacc tgacagtacc gtcatcactt ttgtttacaa aaaagagggc aaggaagtag
     5641 agtttacagc agataaattt cctgcagatt ttagcgccac cacttatgaa tttgtgaacc
     5701 gctatgacaa agtaatccgg aaagggaaaa ataatgaacc tccaataaaa ggatttgcct
     5761 tatcaggaga atcaaatact gattccaccc ggtttgtatt agaacaggag agtgccattt
     5821 tacttttctg tgaaaaattt tccgatgccg gtacggattg gaaaaagaaa tttgaagaaa
     5881 tatatactgc tgccagagca aaaaatattg cctcattcat cattactgcc gaaccaggaa
     5941 aagccaagga tgctttagcc ggaacccggt ttgccgatgt tcctgttttt aaatgtgatt
     6001 ttaaagtaat tgaaacagca gcaagaagta atccttgtat ttattttatt aaaaaaggta
     6061 cggtaataag caaatacagt ggaaaggaaa tgaataatat taaaaagaat atttccgttg
     6121 cgactgccca ggtgagatgg cctttaactc cggcagaaca atctgctgac agtacgatcc
     6181 agcaaccatg ataaaatttg tcaccaaaaa aattgtgtat ggcctgctgg tactggtagg
     6241 agtggtgata ttggtttttt ttctattcca gggttttggt gacccttcaa gaattgtgat
     6301 gggacaaacc ggtgattcaa ctacgcagtc caatatccgt aaagaactgt accttattaa
     6361 tgaaaaagga gaaagtattc caaagttcag gcagtttctt tattacctca atgatgtttc
     6421 tcccatttgc tttcacagca cagcagatat aaacaaaaaa aagctaagag gtttttttat
     6481 tggggaaggg aaaaagtttg gtattaaaat cccttatcta cgaaaatcct atcaaagcaa
     6541 gcgggaagta ggaacgatcc tgatgcaggc attacccggc actttaatgc tcgccattac
     6601 tgccatgttt atcgccgtcc tcatcggcat tccattggga gtaatggctg ctgtgaaaca
     6661 aaatacctgg ctggacacaa cggctatttt cagcagtatt gtgggtatat ccgccccttc
     6721 atttttcatg ggtatcatca tggtctattt gtttggtttt gtcttgagtg attggacagg
     6781 tctgcatatt gccggtagct ggtttgcaat tgatgatgaa ggacaacggc ggttaacaat
     6841 gcaaaatctc attttacctg ccattacatt gggtatccgt ccattggcta tcatcacaca
     6901 attaacacgg agcgccatgc tggatgtact tgaccaggac tatatccgta ccgcttatgc
     6961 aaaaggtttg agcaaaagaa cagtgatctg gaaacatggc ttacgcaatg cattaaaccc
     7021 ggttattact gccattactg gttggtttgc tgaattatta gccggtgctt tctttgtaga
     7081 gtatattttc ggctggcagg ggataggaaa agttacagta gatgcgctgg aaaaactgga
     7141 ttacccggta gtgatgggtt cggtattggt ggccgcaacc ttctttatca tcattaatat
     7201 tcttgccgat attttatacg gcgttgttga tccgagagta agggttgagt aaatgcagtc
     7261 cttaaattta acataagggt catttgatta tctatttctg cacattcgtt gcctgatgaa
     7321 tttttggctt ctttattgcg taactttaag agggagtatt tactttccca attcattaga
     7381 atcatacctt aaaccaaatg tctatggaaa aagtaacttc tatcctcgaa aggaagcaat
     7441 ttcactttaa aaaaatttcc ccttcctgtt ccatcagcga tgccttatgc cggatgagta
     7501 gtcataatac ggactacctg atcgtaatgg atgacaatga taattttctc gggttgctga
     7561 ccgaacacga tgtggccagt aaaacaatat ttctaaaaca gccgcttgca aaagcaagtg
     7621 taaatgaaat gatgaatacc cgtttgccct ttgcagatgt ggatgatacc gtggaagaat
     7681 gtatgaaggc tatgaaaagg tatcatgtaa ggtacctgcc ggtatttaaa aacctggtac
     7741 tgatgggcat tgtatctaca gatgatatcc tggatgaagc ggtcagtcac agaaatgaaa
     7801 tatttgacta accccaaaaa ttatttgccc ccggaaccag tgattattgt atgatcactt
     7861 cattaattac tttctgcttt agtgcctcat tcacccgttg tttaattttt tctttctggt
     7921 agatcagttc attcttgagt ggagcaacat tggttgtgat gattaattta tcaccaatga
     7981 tctgtaattt gtcagtatag cgggaaatgg tctttcccat gatctcttcc catacatctt
     8041 caatctgcaa tgcctgtatg ccgcctttga tacgactgcc gttgaggaat tgctgcattg
     8101 cttcgctgat agaatattca cccataatgc aaagttacaa tgtgatcaat tgaaaattgg
     8161 tattaagttc tttcaaatga gcgataagcc ggtcttcgtt tgtatcggta ataaacacct
     8221 gcccgtcatt ttccacacaa accttattca gcaggttact gatgcggtct tcatcaagtt
     8281 tttcaaacac atcatccagc agcaggaggg gagcaaatcc cttttctttt ttcaatacgt
     8341 ccatttccgc caatttaagt gcaaataata aactcttacg ctggccctgt gaggcaatat
     8401 tcttaaataa ctggttaccc aattgtatat ccaggtcatc acggtgtata ccggatgtgg
     8461 tgcgttgagc aaggcaatct ttttgacggt ttacgcttaa taattctgcg aaagaagcca
     8521 gcaataattc actttcatag atcagtgtca cattttccgg ctgcctggca atatcattat
     8581 ataaatgttt tactgctggt aaaaaactaa ccaggaattc tttacgcttt tcaaaaatat
     8641 attccccggg tttgatcagt tggccgtcca gtacttcaag taatgtaaaa tccctgctgt
     8701 ttgtttctgc aaaatttttc agtactgaat tacgttgttg cagcactttc atgtatatga
     8761 tcaggttttg taaatacgct gtatccaatt gggataataa agcatccagg aatgttcttc
     8821 tttctttact gtcgccggta ataagttccg catcatcagg cgcaatcacc acgcagggat
     8881 aacggccgat atgctgcgag agtttttcat attgctcatc attaactgaa aattcttttt
     8941 ttcctgtttc ccgcaatatg caaacagttt tttcaggttt accgtttaat tccagtttcc
     9001 cctcaatcct gaaaccagca ctgccatgtt ttacattatt cccgtcaagc cgggtaaagt
     9061 agcttttggt gaaacagagg tagtgaatgg catccaacag gttggttttt cccaccccgt
     9121 ttttaccgca gatgcctgca atcctttctg taaagtcaaa agacttattc gtatagtttt
     9181 tgaactgata gagcgatatg tgttgaagga gaagcattaa gggctgcaag atagggtgga
     9241 agggactagg tacaaggcgg aagttccgtg gaaaacagca aatatttgaa cagcttaaag
     9301 atctttat
//
LOCUS       DAORTI010000131         9257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8053_length_9257_cov_5.169420, whole genome shotgun sequence.
ACCESSION   DAORTI010000131 DAORTI010000000
VERSION     DAORTI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_9257_cov_5.169420"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2168)
                     /locus_tag="PLO70_13620"
     CDS             complement(156..2168)
                     /locus_tag="PLO70_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75554.1"
                     /translation="MNRLFPLKLLISILILAVLCCITGKLFSQEYRRWQINEDGSISW
                     IINNNIPHDDHIEMSGKKISCVLRYGVHADSSFHATRSLVWPMLRTIPNNTHASLTRQ
                     FPLDAFDLVTVNYKPIIAEKVVSISIKGTLHVKSIAGNNLELTREYFPSTSLPVYCEV
                     YTIKNKSKNGCLVEIPKVNNIYYTDPTKGTEGSYALHVDVFNVGHFQLQTNDSISFSV
                     FYSGVNANEQVPVADVYHEKEKRLEFVREVWKNLVLETPDTVLNREFAFAKIRAAESI
                     FETKGGPMHGPGGESYYAAIWANDQAEYIGPFFPFLGYAYGNEASLNAYLHFARFINK
                     EYKPIPSSIIAEGTDIWNGAGDRGDGAMIAYGATRYALACGDLKQAAQLWPLIEWCLE
                     YCRRKLNKEGVVSSDTDELEGRLPSGSANLCTSSLYYDALIFAAYLGEALGKEKKQLA
                     LYKKQAVQLKQAIEKYFGSKIGGFDTYKYYKENDTLRAWICIPLTMGIYERKAGTIAA
                     LFSPGLWTEDGLASLAGDKTFWDRSTLYALRGVLASGETDQALRFLHNYSNRRLLGDH
                     VPYPVEAYPEGNQRHLSAESGLYCRIFTEGLFGMRPTGLYSFNFTPRLPSSWPRMSLR
                     HIRAFEHDFDIIVKRAGGKLKVTLSEGEKIIMTKLIKDGDLVKVHF"
     gene            2578..3402
                     /locus_tag="PLO70_13625"
     CDS             2578..3402
                     /locus_tag="PLO70_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ75555.1"
                     /translation="MKKKIVLIKSIRSASGVILLLFLLSSCTKELFNSGITKEFTLQS
                     SASGATYNIQVGLPYNYNPGTEKYETIYVLDGKEIFGFVANRCREISDNYGVKNVLVV
                     SIGYGKDRSIDYTPTKISSITGGAPQFLNFIKTQLIPKMEQDYHADTSRESRVILGHS
                     YGGLFGGYAFCVNNKVFGNYLILSPSFWFDNQVALQMERDNRATNRNRKQLVFMGIGE
                     NEETDRMLAPFETFYQSLRINYTNIKLEKNIEKNQNHMSSRNPNIIRGLEYYFQNR"
     gene            3568..4251
                     /locus_tag="PLO70_13630"
     CDS             3568..4251
                     /locus_tag="PLO70_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75556.1"
                     /translation="MEKNIAILIADLSGYTALTETHGPATAADLIDRYIRIAKNCLVG
                     DSSIHERRGDEIMFVSDSPEALLATALKLEAKTAYEEYFLQVHGGLHFGKVLKRDNSY
                     FGSTINLVSRIAAKAAPGTFWCSNDFMDSVENKSVCNFLSKGNHTFKNIKEEIRVFEL
                     QADRFNSNSIDPVCRMVIVDRQNAIYYPDQAGHYFCSAQCVELYRDNHRHHQTFPDKM
                     EDKRNQIFN"
     gene            4275..4694
                     /locus_tag="PLO70_13635"
     CDS             4275..4694
                     /locus_tag="PLO70_13635"
                     /inference="COORDINATES: protein motif:HMM:NF039957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75557.1"
                     /translation="MTPVLIGISSGLLIILVFVMLKQFDKKLIYGLILCGIGFLYVGF
                     AWSDTRALAINSAQAVLFVFIAYYGMKKSSTILAAGYFLHGIWDLLYHIFQDSGLIPP
                     EYDLFCLSIDFTMGFYILTIKYKAVNKPDLLNPSTED"
     gene            4703..5074
                     /locus_tag="PLO70_13640"
     CDS             4703..5074
                     /locus_tag="PLO70_13640"
                     /inference="COORDINATES: protein motif:HMM:NF023903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HQZ75558.1"
                     /translation="MKFYWSMPEGLKVHYRKELETYKLETSKANFTVAWRHLERAHIL
                     GQPWFVQHTQVHWLMLKFGIRIKNRKEILGQLPRLLVGGIKSFVGTIPTGNTGGANVP
                     PLRVMEIPEELKDIMRPYRNI"
     gene            5111..5677
                     /locus_tag="PLO70_13645"
     CDS             5111..5677
                     /locus_tag="PLO70_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ75559.1"
                     /translation="MKLHIKNMVCDRCVLAVEEILQNEAIPFHNVIYGEVHLQNELIP
                     DQKERLIKRLDKIGFGLIDNRSAAIIEHIKQLIIKRARNENSDYDNKVKLSHYISGQV
                     NLEYTYLSHLFSSIEGRTIENYFIEHRIERAKELLVYGQMTLSQIAFDLAYSSVAHLS
                     CQFKKVTGLTPTYFRDIGMAKRKSLDKI"
     gene            6027..6941
                     /locus_tag="PLO70_13650"
     CDS             6027..6941
                     /locus_tag="PLO70_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQZ75560.1"
                     /translation="MKTVAFIIPPTVELLDLAGPVQVFTEAKIYGMEIDLQFYCYSEA
                     PVSTAGLGFGHIANFKMAKLKAGDFVFVPGMDNDYVNSIPFKAEREFFNWLKECSDQS
                     ITVCSICSGAFALGHAGLLKDTECTTHWRRVDALQQQFPRSKVVADILYIKSNNVYTS
                     AGISAGIDLALAILEELKGALFTHKVARGLVVYHRRSGTHKQQSIYLDYRNHINPQIH
                     EVQDYLIDNLSKENDIESLAAMVGMSPRNLSRVFKEKTGSTVLEYLTLLRKEYASTML
                     NNPEYTIEYIASKCGFKTARQLQRILKK"
     gene            7031..7984
                     /locus_tag="PLO70_13655"
     CDS             7031..7984
                     /locus_tag="PLO70_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQZ75561.1"
                     /translation="MKAAVYFQYGTPQVLRIEDVEKPTPKGNEILIRVKATAVNSGDW
                     RLRKAEPFAVRFIFGLFKPKIKILGSVFSGVVESVGKNVKLFKVDDFVFGHTDMSFGS
                     YAEYKCLPENGSIALKPANISHNEAAVIPFGGVTALHFIKKATIKPGQKVLVVGASGA
                     VGSAAVQLAKSFGANVTGVCSTANIALVKSIGADKVIDYTKEDFTRNGETYDVIFDAV
                     KTISVSGSLKSLNRNGIMILSAAGISETLHGLWISRTNKKKVLTGVISHTAADIIYLK
                     ELIEKDKFKPVIDRTYMLEQIAEAHAYVEKGHKKGNVAIEV"
     gene            complement(8272..8943)
                     /locus_tag="PLO70_13660"
     CDS             complement(8272..8943)
                     /locus_tag="PLO70_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75562.1"
                     /translation="MTEYIYPVKSDTSNYICKVYYENGRLKHETTISNNKFVGEKKSF
                     HENGNLERIEKLYQPTPLNTEIYDCYIINYRTDGSKESEYQYRNDKLNGIAIDYDSTG
                     KKARKAEYIDGKMNGKEIIYYPNGRIKSIVECINDSAYGYEYEFNELGDTLTANIHYG
                     QSDNGVFYKKWLSNGQLLTGSYGDSNRSFVIWNWYDKRRRFIKSLTDKGVFVDSITNR
                     FITPE"
BASE COUNT         2864 a         1755 c         1817 g         2821 t
ORIGIN      
        1 tccagcccga accgctgagg cgacattgct gatgctaaag agttgatagt cctgcactgc
       61 ttttgcaaat accgtgttgt cagaggtatt tcataatagc aactaaataa ttgcaccggc
      121 attaaaacca tctatgttta ccgattattg ccattttaaa aatgaacttt tactaaatct
      181 ccatctttaa tgagtttagt cataatgatt ttttctcctt cggataaggt cactttcagc
      241 tttccacctg ctcttttaac aataatatca aagtcatgtt caaatgcccg gatatgccga
      301 aggctcattc ggggccacga tgagggaaga cgtggtgtaa agttgaaact gtacagaccc
      361 gtaggcctca tgccaaacaa cccctctgta aagatcctgc aatacaagcc gctttctgca
      421 gaaagatggc gttggtttcc ttccggataa gcttctacag gataaggaac atgatctccc
      481 aataggcggc ggttggaata attatgcaaa aaacgcaacg cttgatccgt ttcaccgctt
      541 gccaatacgc ctcttaaagc atataaggtt gatctgtccc aaaatgtttt atcaccggca
      601 agactggcca acccatcctc agtccataat cccggtgaga acaaagcagc gatggttcct
      661 gccttacgtt cataaatgcc catcgttagc ggaatgcaga tccaggctct cagagtatca
      721 ttttctttgt aatatttata ggtatcaaat cctcctattt ttgaaccaaa atatttttca
      781 atagcctgtt tcaattgtac agcctgcttc ttataaagtg caagttgttt tttttccttg
      841 cctaaagctt caccaaggta tgcagcaaaa atcaatgcat cataatataa agaagaagta
      901 cagagattgg cgctccccga aggaaggcga ccttctaatt catcagtatc ggagcttacc
      961 acgccttcct tatttaattt ccgccggcaa tattcaagac accattcaat caatggccat
     1021 aattgtgccg cctgtttcag atcaccgcaa gcaagtgcat aacgggtggc accgtaggca
     1081 atcattgcac cgtctccacg gtcgccggct ccattccaga tatctgttcc ttcagcaata
     1141 atcgaacttg gaataggttt gtactctttg tttatgaatc ttgcaaaatg aagataggca
     1201 ttcaatgatg cctcatttcc atacgcataa ccaagaaagg gaaagaaagg tccaatgtat
     1261 tcagcctgat cattggccca tatggcggca taataagatt ctccgcccgg cccgtgcatc
     1321 ggaccacctt tggtttcaaa aatactttcg gccgccctta ttttagcaaa tgcaaattct
     1381 ctgttcaata cggtgtctgg tgtttccaat acaagatttt tccaaacttc acgaacgaat
     1441 tcaagacgtt tttctttttc atgatataca tccgccacag gtacttgttc atttgcgtta
     1501 accccggaat aaaaaacaga gaaactgata gaatcatttg tctgaagctg gaaatgccct
     1561 acgttgaaaa catcaacatg caaagcataa cttccttccg ttcctttagt gggatcggta
     1621 taataaatat tattcacctt tggtatttct accaggcagc catttttgga tttattctta
     1681 atagtgtaaa cttcacagta aaccggcaat gatgttgatg gaaaatactc ccgggtcaat
     1741 tcaagattat taccggctat acttttaaca tgcaatgttc ctttgatcga tatagaaact
     1801 actttttcag ctattatggg tttatagttt acagttacca aatcaaatgc atccaatggg
     1861 aactggcggg tgagactggc atgtgtatta ttaggaatcg ttctcagcat tggccacaca
     1921 aggctgcggg tcgcatgaaa agaactatct gcatgtacgc catacctgag aacgcaggag
     1981 attttttttc cgctcatttc tatatgatcg tcatgcggga tgttgttgtt gattatccac
     2041 gaaatggagc catcttcatt tatttgccag cggcgatatt cctgcgaaaa caatttgcct
     2101 gttatgcaac aaagaacagc caaaataagg attgaaatca acagctttag cggaaacagt
     2161 ctgttcatta tgttaataca agtttacagt tatctcttaa ggaacgaata aaaacatatt
     2221 taggtatttt tctattacgc cacaaaaatt aaacaactac tcaataatat ttctgctaac
     2281 ggtagggctt gccgaagtgg ggatatagaa ttccgtccgc ccggctaact gaagctaaat
     2341 ttataaataa aagttgatga tatggacaca gctaaattaa taacgtcagg ctggatatga
     2401 agccaaattg aaaagtaacc gactcgtcct aaaattagtt gactggtttt aggtaataat
     2461 cctgttgtat gttcgccctt cgtcttggta attcgaattg atgtctgctt ttgacatcat
     2521 catgtcccac gcagcggaaa aagccaggat atttttgtct ttcaaaaaat aagaaaaatg
     2581 aaaaagaaaa ttgttttgat taaaagtata cgatctgctt caggggttat actgcttttg
     2641 tttttgttga gttcctgtac aaaagaatta tttaactccg gtataaccaa agagtttact
     2701 ttacaatctt ctgctagtgg tgctacttac aatatacagg taggattgcc ctacaattac
     2761 aatcctggta ctgaaaagta tgaaacgatc tatgtgctgg atggcaaaga aatttttggc
     2821 tttgttgcca acaggtgcag ggagatttca gacaattatg gagtgaagaa tgtgctggtg
     2881 gtaagcattg gctatgggaa agaccgtagc attgattata ccccaacaaa aatcagctcc
     2941 attaccggtg gagccccgca gttcctgaat tttattaaaa cgcagttgat ccccaaaatg
     3001 gaacaggatt atcacgcaga tacttccagg gaaagcaggg taatacttgg ccattcgtat
     3061 gggggtttat tcggtggtta tgctttttgc gttaacaata aagtatttgg caactacctt
     3121 atcttaagtc cttctttctg gtttgacaat caggttgctt tacaaatgga gagggataac
     3181 agggcaacta acaggaacag aaagcagtta gtgtttatgg gtattggaga aaacgaagaa
     3241 actgacagga tgctggcccc gttcgaaaca ttttaccaat ctcttcgtat caattatacc
     3301 aatataaagc tggaaaaaaa tattgaaaag aatcagaatc acatgagttc aagaaaccca
     3361 aacattatca ggggattgga gtattatttt caaaacaggt aatagttttc ggattcaacc
     3421 agcatttatt ttttcttaaa atgccccttt tcaaaagggg cattttgttt taccgtcgca
     3481 tgctatgttc ttgtccgttt ttgacatcct catgtctgtt atggtcttta taccccgtct
     3541 atctttacta tacaaaccaa caagcatatg gaaaagaata ttgccatcct gatagctgat
     3601 ttatccgggt atactgcatt aacagaaacg catgggccgg ctacggctgc cgatttaata
     3661 gatcgctata tccggatcgc caaaaattgc ctggtgggag acagcagcat acacgaacgc
     3721 aggggagatg aaattatgtt tgtctctgat tctcccgaag ccctgctggc aactgcactt
     3781 aaattagagg ctaaaaccgc atatgaagaa tatttcctgc aggtgcacgg agggcttcat
     3841 tttggtaagg ttttaaaaag ggataatagc tattttggtt ctacgatcaa cctggtgtca
     3901 agaattgcag caaaagccgc accgggtact ttctggtgtt caaatgattt tatggattct
     3961 gttgagaata aatctgtttg caacttctta tcaaaaggca atcatacgtt taaaaatatt
     4021 aaagaggaaa taagggtttt tgagttacag gcagatagat ttaacagcaa ttctattgat
     4081 ccggtttgcc gaatggtaat agtagaccgt caaaatgcaa tttattatcc tgatcaggcc
     4141 ggtcattatt tctgctctgc gcagtgcgtt gaattatatc gggataatca cagacatcat
     4201 caaacttttc ctgataaaat ggaggataaa agaaatcaga tttttaatta aatcaacatt
     4261 aaaaaatcaa cgtcatgaca ccagttttaa taggcatttc atctggtttg ttaatcatcc
     4321 tggtttttgt tatgctgaag cagtttgaca aaaaactgat ttacggtctg atactttgtg
     4381 gaatcgggtt cctttatgta gggtttgcct ggtcagatac ccgggctttg gctattaatt
     4441 cagcccaggc cgtcttgttc gtctttattg cttactatgg gatgaaaaaa agtagtacca
     4501 ttttagccgc cggatatttt ctgcacggta tctgggattt actataccac attttccagg
     4561 attcaggttt gatcccgccg gaatacgacc tgttttgcct gtcaattgat ttcaccatgg
     4621 ggttttatat tcttacgatc aaatataaag ctgtaaataa accggatctg ttaaaccctt
     4681 cgactgaaga ttaattttta aaatgaaatt ttactggtca atgccggaag gtttgaaagt
     4741 ccattaccgt aaggagcttg aaacctacaa gctcgaaacg tctaaagcta actttactgt
     4801 tgcctggaga catttggaaa gagcccatat tctcggccag ccctggtttg tacaacacac
     4861 ccaggtacat tggctgatgc tcaaattcgg catacgaatc aagaatagaa aagaaatcct
     4921 tggccaacta ccaagacttc tggttggagg gattaaatct tttgttggaa caattccaac
     4981 cggcaatacc ggcggggcca atgtaccccc cctgcgggta atggaaattc cggaggagct
     5041 taaagatatc atgaggccgt atagaaatat ttaaaaaaaa tatctgaaca aaactgaatc
     5101 aattactggc atgaaacttc acatcaaaaa tatggtttgt gatagatgtg ttcttgccgt
     5161 tgaggaaatt ttacagaatg aagcaatacc ttttcacaac gttatatatg gcgaggttca
     5221 tttacaaaat gaattgatcc cggatcaaaa ggaacggttg ataaaaagat tggacaaaat
     5281 aggattcgga ttaattgata accgaagtgc tgcaataatt gaacacatca agcaattgat
     5341 catcaagaga gccagaaatg aaaatagcga ttacgataat aaagttaaac tttcccacta
     5401 tatatccggc caggtcaatc ttgaatatac ttacctgagc catttatttt cttcaataga
     5461 aggaagaaca atagaaaatt attttattga acaccggatt gagagagcaa aagaactact
     5521 ggtgtacgga caaatgactt tgtctcaaat tgcttttgat cttgcgtaca gcagcgtagc
     5581 tcatctttct tgtcagttta aaaaggtaac agggttaacc cccacttatt ttagagacat
     5641 tggtatggca aagcgtaaat cattagataa gatttaatct aaaaattata caacacatcc
     5701 ccaaagtaat gtaacaatca ccactctttc tcaacctatt tttgaagtat taaataacat
     5761 aaaaaacaag aattatgaaa tcaacaacaa acatgagtca aagtactcag aacgtaaaat
     5821 gcagtaatcc taattgtacc tgtgacaatt gtaattgcgg tgctaactgt acctgctcca
     5881 attgtaaata agcaattaag gttttacatt tgaccagccg ttccccgggg atcggctttt
     5941 ttatgtcttt ttctgacctt ttgttggctg tttttctact cactcttcag cctaccttta
     6001 ctttatcaac ttaataaatt cagaacatga aaacagtagc ttttatcatt cctccgactg
     6061 ttgaattact tgaccttgca gggccggtgc aggtttttac cgaagcgaaa atatatggaa
     6121 tggaaataga ccttcagttt tattgttaca gcgaagcgcc tgttagtact gccggccttg
     6181 gtttcggaca tatagctaat tttaaaatgg caaaattaaa agcaggtgat tttgtgtttg
     6241 tgccgggcat ggataatgac tatgtaaaca gtattccatt taaagccgaa agagaattct
     6301 tcaactggct gaaagaatgt tccgatcaaa gcataaccgt gtgttccata tgcagcggcg
     6361 cttttgcatt gggacatgcc ggtttactga aagatactga atgtacaacc cattggagaa
     6421 gggttgatgc tttgcaacaa cagtttcccc gctcaaaagt ggttgccgat atcttatata
     6481 taaagagtaa taatgtttac accagtgcag gcatcagtgc cggtattgac ctggctttgg
     6541 ccattcttga agaactgaaa ggagcattat ttacacataa agtagcaaga ggcctggtag
     6601 tttatcaccg gcggagcggc acacataaac aacaaagtat ttacctcgat tacaggaatc
     6661 atatcaatcc gcagatacat gaagtgcagg attacctgat cgataatctt tcgaaagaga
     6721 atgatataga atcactggct gcaatggtag gaatgagccc aagaaacctg agcagggtgt
     6781 ttaaagaaaa gacaggatca actgttttgg aatatttaac cttgttaaga aaggaatatg
     6841 ccagtaccat gcttaataat cccgagtata ctattgagta tattgcctcc aaatgtggat
     6901 ttaaaacagc caggcaatta caaaggatat taaagaaata aggccggttt catttctgtg
     6961 tattaaaccc tggttggggg tcgatatttt ttatcctcag tcttttaaga taattaataa
     7021 aaccattaaa atgaaagcag ctgtttactt tcaatatggt acgccccagg ttctccgaat
     7081 agaggatgtg gaaaaaccca ctccaaaggg gaatgaaatt ctaatacggg ttaaagcaac
     7141 agcagttaat tcaggtgatt ggcgattacg aaaagctgag ccatttgcgg tcagatttat
     7201 tttcggtttg tttaaaccca aaattaaaat acttggcagt gttttttcag gtgttgtcga
     7261 aagtgtcggt aagaatgtaa aacttttcaa agttgacgat tttgtctttg gtcatacaga
     7321 tatgagtttt ggttcttatg ctgagtataa atgtctgcct gaaaacgggt caatagcatt
     7381 aaagcccgca aatatttctc acaatgaagc agctgtcatt ccttttggcg gagttacagc
     7441 attgcacttt ataaaaaagg caacgataaa accagggcaa aaagtactgg tagtaggtgc
     7501 ctctggtgcg gttggaagtg ctgccgttca attggcaaag tcgtttggag ctaatgtaac
     7561 gggtgtttgc agcactgcaa atattgcttt ggtaaaatct atcggcgcag ataaagtaat
     7621 tgattatacg aaggaagatt ttacccgaaa tggtgaaacg tatgatgtga tttttgatgc
     7681 tgtgaaaaca atatctgttt caggaagttt aaagtcgctg aatagaaatg gaataatgat
     7741 tctaagtgct gcaggaattt ctgaaacgct tcacggatta tggatttcca ggacaaataa
     7801 gaaaaaagtt ttgaccggtg tcattagtca tacagcagca gatatcattt atctaaagga
     7861 actcattgag aaagataaat ttaagccagt aattgacaga acatatatgt tggagcaaat
     7921 tgctgaagct catgcttatg ttgaaaaagg acataaaaaa ggaaatgttg cgatagaagt
     7981 ataacttaat ttacaataac cccggataga aagattaagt cttgggtctt gctaaatatg
     8041 aacgctttac ataaataggg gtacatacgt tcacagcttt gcggtcggcg gggcaattct
     8101 tttacgtcgc ccctggcgac gagtgctgaa ctgtgatact aaagataaaa ttttattcaa
     8161 aagcctacta gataaatgtc agccggaaca aaagctaaat agaattacag gtgatgaccg
     8221 acgctttgcg tcgccccccg ctgccgcaaa aatgcatgta agctgcagct tttactctgg
     8281 agtaataaat ctgtttgtta ttgagtctac aaatacaccc ttgtcggtta aggattttat
     8341 aaatctcctt cttttgtcat accaattcca tataacaaaa cttctattcg agtccccata
     8401 acttccagtc agaagttgtc cattagaaag ccattttttg taaaaaacac cattgtcact
     8461 ttgtccatag tgaatatttg cggtaagggt atcgcccaat tcattaaact cgtattcata
     8521 tccataagca gaatcgttta tacactcgac aattgactta attctaccat ttggataata
     8581 aataatttct ttgccattca tctttccgtc aatatattct gcttttcggg ctttttttcc
     8641 tgttgagtca tagtctatcg ctatcccgtt taatttgtca ttcctatact gatattcgct
     8701 ttctttagaa ccgtcagttc gataatttat gatgtagcag tcatatattt cagtgttcaa
     8761 aggagtgggt tgataaagtt tttctattct ttctaaattg ccattttcat gaaaggattt
     8821 tttctcacca acaaatttgt tatttgaaat tgtagtttca tgcttaagtc gtccattttc
     8881 ataataaacc ttgcatatat aatttgaggt gtcgcttttg actggataga tgtattcagt
     8941 cattgtctgt ccattagaat atctgtcaat aattctttta gtagtcggtt tgcagcctaa
     9001 aaatagaata accagaaatg taataggaaa cgtcctcata aagttgcagc ttacacttaa
     9061 ataaacgcaa tgacttataa attagagaaa atgcaagtgg ttgactttca aataaaatga
     9121 cttgcgtttt ttaaattgtt agttgcggca atacaaatca tattaaataa tagcacgggc
     9181 ctgttcccca ttttgcgggg ttacaatttt tcgtacaagg ctctcaactt ctcccttgtc
     9241 atgatctttt cccaatc
//
LOCUS       DAORTI010000132         9085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8282_length_9085_cov_7.209856, whole genome shotgun sequence.
ACCESSION   DAORTI010000132 DAORTI010000000
VERSION     DAORTI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_9085_cov_7.209856"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLO70_13665"
     CDS             <1..355
                     /locus_tag="PLO70_13665"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75563.1"
                     /translation="QLEENYWKYVKEKDYQSYATLWDDNFLGYPSNNTVGDKAHITNW
                     MTEMYRQPGVFNYTLTRKVENVFGDIAIVFYDVSHHFTNDKNEIVKKGSFKIIHTWKK
                     MAKGWLIIGGMGANK"
     gene            373..1533
                     /locus_tag="PLO70_13670"
     CDS             373..1533
                     /locus_tag="PLO70_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQZ75564.1"
                     /translation="MKFNLTIFLLFIIQTVSIAQKPRARDIGIPFDGTQGKFNAITDV
                     KGVEVGYSTIISGSGKNIRGKGPVRTGVTAILPRGRNNNPVYGNWYSLNGNGEMTGTT
                     WITESGFLEGPIMITNTNSVGVVRDAVLKWYVKTGWYKEDFWYTYPVVAETYDGFLND
                     IYGFHVKESNAFEALESAKSGLLKEGNVGGGTGMMCLGFKGGSGTSSRVVKIKDSSYT
                     LGVFVQSNFGRKRNLTIAGVPVGKALRDTLNNEYKAAPSYRQEGDGSIIVVVATDAPL
                     LPHQLKRIAQRVSLGIGIVGGQGTNGSGDIFIAFSTANMTAFQRESFTKIDVLPNDFI
                     DPLFEATVQATEEAIINAMVAAETMEGINGNKSYGLPHKLVIDILKKYNRVK"
     gene            1556..2314
                     /locus_tag="PLO70_13675"
     CDS             1556..2314
                     /locus_tag="PLO70_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75565.1"
                     /translation="MQSKKFYYHNLGYWFLLFIILVIAGFYTTYFTRILEPTPAVIHI
                     HFVLMTLWLLLLIAQPFLIKYKKLSWHRILGKASYILVPLLLITGFLLIRNEYYRNLE
                     GLNQAVLTGQKQLSQPEILKEIAATPIGLFYLTWFMVFYGLAIRNRRQSPKHARYMLA
                     TALTLTGPTVDRIVGIHFHIYTVAGISSFIISFLIIDIVLALLLFLDYRNKKETKTLW
                     TCLLIYTIGQFLYYLLPTFDWWAEFMKFIMLPKP"
     gene            2396..3829
                     /locus_tag="PLO70_13680"
     CDS             2396..3829
                     /locus_tag="PLO70_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ75566.1"
                     /translation="MKNLIILFCFAAAMLPIGIGTASAQKPNVDSILQKVALEKNEDK
                     KVDLLVSLVSSEINNDPQWGIETGLKLLNQAKRGNNNIELSVAYSFLGQGYRLLGNNI
                     KSLDYHHKGIAAAEKSGNLSILAFAENQTAHIYKDREEYDKAISLYLSATAHADKGKN
                     EKIKGWAPSNLGAVYLATNKLDSSLMYSQRAYEIFVRLKTISNNAFLFTNLGGVHSKL
                     GNTPLAISYFNMAINQSVGTPNIRYLTMGYTGLAEHYQRFNQTDSSVFYAKKAIAVVN
                     NTPFFYLCSKPAELLTDIYEKTNCDSTLKYAKIFKMAADSLNSSKVNQQILLMTFDED
                     LRQQEVAAEKIKEAQQRKQNIQYALLALGIISFVILFLALSRRHITNTKVIQFLGVVA
                     LLVVFEFLNLLLHPFLERITHHNPILMLLALVCIAALLVPLHHKLEKWATHKLVEKNK
                     AIRLAAAKKTIQLLSETPAETDKNKES"
     gene            4059..>9085
                     /locus_tag="PLO70_13685"
     CDS             4059..>9085
                     /locus_tag="PLO70_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75567.1"
                     /translation="MKPDKKQEAEVLKVYDAWWHSYLTGDVKTYDSLLDDEYRFVGST
                     NGEEFLNRKDTTKFFELTADQLAGKAELRNLTRTIEKLDSGHILITDLADAYVISESE
                     WVFYSRFRFTSLMKETKNGWRFIYQHFSAPDTKAQEGETLGTEQITKENQELRDAIKR
                     RTVELENKTRALEIEASLERVRTVAMGMNKPDDMLDICRIISQQLKLLNVKEIRNVQT
                     AIIYEAKGSYLNFEYYAKHEKTFITDVDFKLHPTQFEFVNRMLKGPEEFFNKHFNGKE
                     VQDWYTYQQTTNQFADSYLATATSLNYYWYSMGPVALGISTYVPLTEEDIVLFKRFRN
                     VFELAYRRFIDIEQAAAQARDAQIEAALERVRAKVMAMNSSKELPETSFVFGEQLRKL
                     GIDWQFSYFWLIEEDKDDNTFWITWPDNQTSTTSYSLAEADQNFKDCIVAWKNQEKIH
                     GTHVAAEYVKSWLDTFDRITTDAGGVAADIMKAGNFKDGVYYYDAMIRFGSFGILMNR
                     ATTDEEKNIQSRFATEFERAYTRFMDLQKAEAQAREAQIETSLERVRSRTMAMQKSEE
                     LKEVIKIVYQQLRHLKINLDHAGFVVDYTPGGDWHFWIADERDIPSKITHPYFDSVWA
                     NQFNEAKEKGADFFATHLNFEEKNKFYNELLSYVPGLPDVSKDFYLNCPGLAATTVLL
                     DNVALYIENFSGIPYTEEENSMLMRFGKVFQQTYTRFLDLQKAEAQAREAQIEAALER
                     VRSRSMAMHKSDELLEAGEILFLEMQKLGIASLTAGYVLIDEAEKNGLNYTPHPGTKK
                     IMPVPVIIPHNETIHMQQVVENWKKGNLFFIVEMDEEETIQHQTFIAERSTNFPLTAA
                     QLIAISPARLFLHNFFFKEGYVLIVGGTKLSAEQTDIMLRFAKVFQQTYTRFLDLQKA
                     EAQAREAKIEAALERVRSRSLAMHHSSELSAVVDTLLREFTNLEFTLTFCIINLIDEQ
                     DRSNTVWAANPETGMQPESYYMKFEDYPFHHAMWDAWKEQKKNFIYTLEGKEKKIYDE
                     YLYTKTEFRRFPKHVQEANKALKRYVAGFTFFKYSGLQTVSENQISEDELKILERFGR
                     VFEQSYTRFIDLQKAEAQAREAQIEASLERVRSKTMAMHNSREVGETVATMFEELVKL
                     GVDKSIRCGIGILNDTRHMEVWTASTNKSGETTLDIGLLDMTIHPLLAGVKEAWKNKK
                     TGFIYELAGEDLKNYFIAINAAPDYPVYVDLETLPDKIIHTDFFFSEGTLFAFSSNLI
                     PEETAQVLKRFAGVFGQTYRRYLDLQKAEAQAREAQIEAALEKIRSHTNAMRSSEDLS
                     NVATIMFEQMRILGGDLFSFGIVLCDRHANMVEQWHSIPGAGMIPPFFVPVDLDHIHR
                     YRYDQWKAGVELFSVVIPEDYIKEHFRIMLELPSVKSVIDMMTANKIPLTIPDWEIDY
                     GASFKYGYLLVSSLKPFEEDKIFPRFAKVFEQTYTRFLDLQKAEAQAREAQIEAAMEK
                     VRSRSLAMQKPEELTEVAELLRKEMGQLGVEELETSSIYIVDKENEQAECWYAIKDIR
                     EENKRLVSDEMTITFADTWVGSEMKKFYRAKKQQASIVMKGDNRKEWINYCAEKSNVL
                     QGYYGDEIPERTYHLVKFSGGFMGAASPGDISAESWDLLRRAASAFSLAYTRFNDLQI
                     AEAHALQA"
BASE COUNT         2943 a         1723 c         2021 g         2398 t
ORIGIN      
        1 gcagttggaa gaaaattact ggaagtatgt aaaagaaaaa gactatcaat catatgccac
       61 attatgggac gacaactttc tcggatatcc cagtaataat accgttgggg ataaagccca
      121 tatcaccaac tggatgacgg aaatgtacag gcagcccggt gtttttaatt atacattgac
      181 ccgcaaggtt gaaaatgtgt ttggtgatat tgccatagta ttctatgatg tatcgcacca
      241 ctttaccaac gataaaaacg aaatagttaa aaaaggaagt tttaaaataa tacacacctg
      301 gaagaaaatg gcgaaaggat ggttgatcat tggaggcatg ggtgcaaaca agtaatccaa
      361 aaaataattt atatgaagtt caacttaacc atattcctgc ttttcattat tcagacagtt
      421 agtattgcac aaaaaccaag agccagagac atcggaattc cttttgacgg cacacaggga
      481 aaattcaatg ctatcaccga tgtaaaaggt gtggaagttg ggtacagcac cattatttcc
      541 ggtagtggga aaaacataag aggcaaaggc ccggtgcgaa caggtgttac tgctatttta
      601 ccaaggggaa gaaataataa tccggtgtat ggaaactggt attcattaaa cggaaatggt
      661 gaaatgaccg gtaccacctg gataacagag tctggctttt tagaaggccc catcatgata
      721 accaatacca atagtgtagg tgtggtaaga gatgcggtac tgaaatggta tgtaaaaaca
      781 ggttggtaca aggaagattt ctggtacacc tatcccgtag tggcagaaac ttatgatggt
      841 tttttaaatg atatctatgg ctttcatgta aaagaaagca atgcttttga agcactcgaa
      901 agtgcaaaat ctggtttact caaagaaggc aatgtgggtg gcggcaccgg tatgatgtgt
      961 ttgggattta aaggtgggtc aggaacatcg tcaagggtag taaaaattaa agactctagc
     1021 tatacattgg gggtgttcgt acaatccaat ttcggcagaa aaagaaatct caccattgcc
     1081 ggcgtaccgg ttggaaaagc attacgggat accctgaaca atgaatataa ggcggctccc
     1141 tcttacaggc aagaaggtga tggatctatt atagttgttg ttgcaacaga tgctccgtta
     1201 ttaccacacc agttaaaaag aattgcacaa agagtatcgc tggggatcgg tattgttggc
     1261 gggcaaggca caaatggttc cggcgatatt tttatagcct tctctacagc caatatgacc
     1321 gcctttcaaa gagaaagttt tacaaaaata gatgtgctgc ctaatgattt tattgatccg
     1381 ctatttgaag ccacggtgca ggctaccgaa gaagctatca ttaatgcgat ggtggctgct
     1441 gaaacgatgg aggggatcaa tggaaataaa tcgtatggcc tgccgcataa attagtgatt
     1501 gacatattaa agaagtataa ccgggtgaaa taatttttta tagaaaagct tgtttatgca
     1561 atccaaaaaa ttctactacc ataacctcgg ttactggttt cttctattca tcatattagt
     1621 gattgccggc ttttacacaa cttattttac aagaattctt gaaccaacac cagctgtcat
     1681 tcatatacat tttgtattga tgacactgtg gctgctgctg ctgattgcac aaccattcct
     1741 catcaaatac aaaaaattgt cctggcaccg gatcttaggc aaggcaagct atatattggt
     1801 acccttatta ttaataaccg gtttcctgtt aatccggaat gaatattacc gtaacctgga
     1861 gggtttgaac caggcagtac taaccgggca aaaacaatta agtcagccgg aaatcttaaa
     1921 agaaattgct gcaacaccca ttggcctctt ttatcttacc tggtttatgg tgttctatgg
     1981 tttagccatc cgaaacaggc gccaatcgcc caaacatgcc cggtatatgc tggccaccgc
     2041 cctgacactt accggcccga cggttgacag gatagtgggg attcatttcc atatctatac
     2101 tgttgccggt atttcttcct ttatcatttc ttttttgatc attgatattg tgctggcctt
     2161 attactattt cttgactaca ggaataaaaa ggaaacaaaa acactgtgga cctgcctgct
     2221 gatttatacc atcggtcaat tcctatatta tctattgcca actttcgact ggtgggcgga
     2281 atttatgaaa ttcattatgt taccgaaacc gtaaacgatg aggggagaga ttttaaagaa
     2341 ttattttccg gcgcttagtt ttttcactaa aaaccgggga aaactaacta aaaagatgaa
     2401 aaacttaata atactctttt gttttgctgc agctatgctg ccgataggta tcgggactgc
     2461 atctgcacaa aaaccaaatg tagactccat actgcaaaaa gttgcgttag aaaagaatga
     2521 agacaaaaag gtagacctgc tcgttagttt ggtatcatca gaaattaata acgatcctca
     2581 atggggtatt gaaacaggat taaaattatt gaaccaggca aaaagaggga ataataatat
     2641 tgaactgtca gttgcgtatt cttttttagg ccaggggtac cggttgctgg gaaacaatat
     2701 taaatcgttg gattatcacc ataaaggcat tgctgctgca gaaaaaagcg gcaatttatc
     2761 tattctggct tttgcagaaa atcaaacagc acatatttat aaagacaggg aggaatatga
     2821 taaagctatt agtttgtacc tgtcagcaac agcacatgcg gataaaggga aaaatgaaaa
     2881 aataaaagga tgggctcctt ctaacctggg ggctgtttat ctggctacca ataaattaga
     2941 ttcatcgttg atgtattcac aaagggctta tgaaattttt gtaaggttaa agaccatttc
     3001 caataacgct ttcctgttta caaacctggg tggtgtacat agcaaactgg gcaatactcc
     3061 gcttgccatc agctatttta atatggcaat caatcaaagt gtaggcacac ctaatatcag
     3121 gtatctcact atgggataca caggtttagc agagcattac cagcgattta atcaaaccga
     3181 ttccagtgta ttctatgcaa aaaaagcaat tgcagttgtc aacaatactc cattcttcta
     3241 cctgtgcagt aaaccagcag aactgcttac tgatatttat gaaaaaacaa attgcgacag
     3301 cactttgaaa tatgcaaaga tttttaaaat ggctgctgat tcattaaaca gcagcaaagt
     3361 caaccagcag attctattaa tgacttttga tgaagacctg cggcagcagg aagtagcggc
     3421 agaaaaaata aaagaagcac aacagcgtaa acaaaatatt caatatgcat tactggcttt
     3481 aggtatcatc tccttcgtta ttctattttt agcactcagc cgtcgtcata ttaccaatac
     3541 caaagtcatc cagtttttgg gagtagtagc tttactggta gtgtttgaat ttttaaattt
     3601 actacttcat ccattcctgg aaagaatcac tcatcacaac cctatcctga tgctgttggc
     3661 attggtttgt attgctgcgt tgctggttcc attacatcat aaattagaaa aatgggcaac
     3721 acataaactg gtggagaaaa ataaggcgat acggctggca gcagcgaaaa aaacgatcca
     3781 actactctct gaaaccccgg cagaaacgga taaaaacaag gaaagttgat taacttcctg
     3841 cactaataag atgatccgcc cgacgtcacc cttttgttac tttttccggg ttggcgcatc
     3901 ggttgaaggg ctatcgtatt tcccggcaag tggccccgat gggcaaagat gtaccaataa
     3961 aatgcctccc tctgaaacaa ccccctttta ttttaataat ggaacaaata aatgattttc
     4021 ccagggctat tcatttgcaa accaattgtc aataagtcat gaaaccagac aaaaaacagg
     4081 aggcagaagt tttaaaagtg tatgatgcct ggtggcatag ctatctgaca ggcgatgtga
     4141 aaacctatga ttcattgctg gatgacgaat acaggtttgt tgggtccact aatggggaag
     4201 aatttttaaa cagaaaggat acgacaaaat ttttcgagtt aacagccgat cagctggcgg
     4261 gaaaagcaga actaagaaac ctgaccagga ccattgaaaa gctggatagc ggacatatcc
     4321 tgattaccga tctggcggat gcctatgtta tcagtgaatc ggaatgggtt ttttattcaa
     4381 ggttcaggtt tacatcattg atgaaggaaa caaaaaatgg ctggcggttt atttaccagc
     4441 atttctctgc accggataca aaagcacagg aaggggaaac gttgggaact gaacagataa
     4501 caaaggaaaa ccaggaactc cgggatgcca ttaaaagacg tacggttgaa ctggaaaata
     4561 aaaccagggc actggaaatt gaagcttccc tcgaaagagt aagaaccgtt gccatgggga
     4621 tgaacaagcc ggatgatatg ctggatattt gccgcatcat ttcccaacag ctgaaattac
     4681 tgaatgtaaa agaaattcgt aatgtacaga cagccatcat ttatgaagcc aaagggagtt
     4741 atctcaattt cgaatactat gccaagcatg aaaaaacatt tatcactgat gtagatttca
     4801 agctgcaccc gacacagttt gaatttgtaa atcggatgct caaaggcccg gaagaatttt
     4861 tcaataagca ttttaacggt aaagaagtac aggactggta tacctaccag caaaccacaa
     4921 accagtttgc agactcatat cttgccaccg ccacttcttt aaattattat tggtattcca
     4981 tggggcctgt ggcactgggt atctcaacct atgttccatt aacagaagaa gacattgttt
     5041 tattcaaacg tttccgcaat gtgtttgaat tagcctatcg ccggtttata gacattgaac
     5101 aggctgcagc gcaggcaaga gatgcacaaa ttgaggcggc gctggaaaga gtgcgggcaa
     5161 aagttatggc gatgaacagt tcaaaagaac tgcctgaaac atcttttgtg ttcggcgagc
     5221 agttgagaaa gctcggaata gactggcagt tctcctattt ctggctgatc gaggaagata
     5281 aagatgacaa taccttttgg ataacctggc cggataacca aacaagtaca acaagctatt
     5341 cgctggctga agctgatcag aactttaaag actgcattgt tgcatggaaa aaccaggaaa
     5401 aaatacacgg cacccatgta gccgcggagt atgtgaaatc ctggctggat acatttgatc
     5461 gaatcactac agatgccggt ggtgtggccg ctgacataat gaaggccggt aattttaaag
     5521 acggagtgta ttattatgat gccatgatcc gattcggttc gttcggaata ctaatgaacc
     5581 gggccacaac cgatgaagaa aaaaatatcc agtcccgttt tgcgacagag tttgaaagag
     5641 cctatacccg tttcatggat ctgcaaaaag cagaagcaca ggcaagggaa gcacagattg
     5701 aaacttcatt ggaaagagtt cgttccagaa ctatggcgat gcaaaaaagt gaagagctta
     5761 aagaagtaat taaaatcgtt taccagcaac tcaggcattt aaaaattaat ttagaccatg
     5821 caggttttgt tgtggactat acaccgggag gcgattggca tttttggatc gctgatgagc
     5881 gagatattcc ttctaaaata acccatccgt attttgattc tgtttgggca aatcaattta
     5941 acgaggcaaa ggaaaaaggc gcagattttt ttgccacgca tttaaatttt gaagaaaaga
     6001 acaaatttta caacgagctt ttatcctatg ttcccgggtt accggatgta tcaaaagatt
     6061 tttatttaaa ctgccccggg ctagcagcaa cgaccgtatt gctggataat gtggccttgt
     6121 atatcgagaa cttttcaggt atcccttaca cagaagaaga gaatagtatg ctgatgcgtt
     6181 ttggaaaagt attccagcaa acctataccc gttttcttga tctgcaaaaa gccgaagcac
     6241 aggcaagaga agcacagata gaagcagcat tggaaagggt aagaagcaga agtatggcca
     6301 tgcataaaag tgatgagtta cttgaagctg gtgaaattct ttttttagaa atgcaaaaac
     6361 tgggtattgc aagcctgacc gccggttatg tgttaattga tgaagcagaa aaaaatgggt
     6421 tgaattacac ccctcatccc ggtacaaaaa aaataatgcc ggtaccggtg atcattccgc
     6481 ataacgaaac catccatatg caacaggtgg tggagaactg gaagaaaggc aatctttttt
     6541 tcatcgttga aatggatgaa gaagaaacta tccaacacca gacttttata gcagaacgca
     6601 gtaccaactt tccgctaacg gcagcacaat tgattgccat ttcgccggcc agattgtttc
     6661 tgcataattt cttttttaaa gaagggtatg tattgatagt ggggggtaca aaattatctg
     6721 cggagcaaac agatattatg ttgcgttttg cgaaagtgtt tcaacagacc tatacccgtt
     6781 ttctcgatct gcaaaaagca gaagcacagg caagagaagc aaaaattgaa gctgccctgg
     6841 agagggttag atcccggagc ctggcaatgc atcattcctc cgagttatct gcagtagtgg
     6901 atacacttct cagagagttt actaatcttg aatttacgct taccttctgt atcatcaacc
     6961 tgatagatga gcaagacagg agcaatacgg tttgggcggc taaccccgaa acgggaatgc
     7021 aaccggagtc ctattatatg aaatttgaag attatccctt ccatcacgcc atgtgggatg
     7081 cgtggaaaga gcagaagaaa aattttatct acaccctgga ggggaaagag aaaaaaatct
     7141 atgatgaata cctatatacc aaaactgaat tccggcgttt ccccaaacat gttcaggaag
     7201 cgaataaagc cctcaaaagg tatgtggcgg gctttacatt cttcaagtat agcggactcc
     7261 aaacagtgag tgaaaaccaa atatcagaag atgaattgaa aatcctcgaa cgtttcggca
     7321 gagtcttcga gcaatcctac acccgattta ttgatttaca gaaagcagaa gcacaggcaa
     7381 gagaggcaca gattgaagca tccctggaaa gagtgaggag caagacgatg gcgatgcaca
     7441 atagccggga ggttggcgaa acagttgcca ccatgtttga agaattagtg aaactgggag
     7501 ttgataaatc aatccgctgc ggtattggca ttttaaatga taccaggcat atggaggtat
     7561 ggacggcctc aacgaacaaa agcggagaga ccacattgga tatagggctg ctggatatga
     7621 ccattcatcc cttattagca ggtgtgaaag aggcatggaa aaataaaaag accgggttta
     7681 tctatgaact ggctggtgag gatttaaaaa attatttcat agctattaat gcggcacctg
     7741 actacccggt ttatgttgat cttgaaacat tgcctgacaa gataatccat acagatttct
     7801 ttttttcaga gggaaccttg tttgcttttt catccaacct cattccggaa gaaacagccc
     7861 aggtgcttaa aagatttgca ggggtatttg gccaaaccta ccggcgatac cttgatctcc
     7921 aaaaagccga agcacaggca agagaagcac agattgaagc ggcattggaa aaaatccgtt
     7981 cacatacaaa tgctatgcgt tcgagtgagg atctttcgaa tgtagcaacc ataatgtttg
     8041 aacaaatgag aattctgggt ggtgacctgt tttcatttgg tattgtgctt tgtgacaggc
     8101 atgcgaatat ggttgagcag tggcatagca ttcccggtgc cggcatgata ccacctttct
     8161 ttgtacctgt agatcttgat catattcatc gctaccggta tgatcagtgg aaagccggtg
     8221 ttgaattatt ttctgttgtt ataccggaag attatataaa agagcatttc aggatcatgc
     8281 tggagctgcc ctcggtaaaa tctgtgatag atatgatgac ggccaacaag attccattaa
     8341 caatacctga ctgggaaatt gattatgggg cgtcattcaa atacggctac ctgcttgtat
     8401 catcattaaa gcctttcgaa gaagataaaa tatttccacg atttgcaaaa gtattcgagc
     8461 agacctatac ccgttttctc gatctccaaa aagcagaagc acaggccagg gaagcacaga
     8521 tagaagcagc aatggaaaaa gtccgttccc gctctctggc tatgcagaaa cccgaagaat
     8581 tgaccgaagt agcagagtta ttacgaaaag aaatgggaca attgggtgtg gaagaattgg
     8641 aaacaagttc tatatacatc gttgataaag aaaatgagca agccgaatgc tggtacgcca
     8701 ttaaagacat cagggaggaa aataaaagat tggtaagcga tgaaatgacc atcacattcg
     8761 ccgatacatg ggtgggcagt gaaatgaaaa aattttaccg ggctaaaaag cagcaggctt
     8821 ccattgtaat gaaaggcgat aacaggaagg agtggataaa ctattgcgca gaaaaatcaa
     8881 acgtattaca gggttattat ggtgatgaaa tcccggagcg tacttatcac ctggtaaagt
     8941 ttagtggtgg ttttatggga gctgcatcac ccggcgatat ttcagcagaa agctgggact
     9001 tactgcgaag agccgcttcc gctttttcat tggcctacac ccgttttaat gatttacaaa
     9061 tagcagaagc acatgccctg caggc
//
LOCUS       DAORTI010000133         8909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8520_length_8909_cov_8.115767, whole genome shotgun sequence.
ACCESSION   DAORTI010000133 DAORTI010000000
VERSION     DAORTI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8520_length_8909_cov_8.115767"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2578)
                     /locus_tag="PLO70_13690"
     CDS             complement(<1..2578)
                     /locus_tag="PLO70_13690"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ75568.1"
                     /translation="MRKIYLLVLTFFVATLLLAQKNGAVKGLAFDTISKQPVSAATIT
                     VLERKDSSLVTFTMTGSDGRFELKGLANGDYRLLITHVNYHNSNKYFSISDAHKNADM
                     GNLVMNDKAKVLEEVVLANEAPPVTLINDTIQYNAGSFKVQPNASVEQLLKKLPGVKV
                     EKDGTIKAQGETVSRVLVDGKEFFGNDPKIATKNLPADAVDKVQVYDKQSDQAQLTGF
                     EDGNYEKTINLKLKKDKKKGLFGKVNAGAGNKERYEGKFNVNSFKGARQFSAIGMGNN
                     TNAEGFSFMDILNFTGEMARMQRGGGGNININISSQDAAAMGMNSGNRNSGINTAWGG
                     GLNYNNIIGTKLDFQSNYFYNRYNPNTESHIQRQYILPDTSYYNANTYSDNLNNTHRL
                     NLNMLYQLDSMNSIRITPSFSYQKTSNRSQSDYQTLSEQQGLINEGFSNNLSNSEGYN
                     FRNDIIWRRKFARKGRTFSLSLQTSLNESEGDGSLSSVNSFYAPGGSLLRKDTLNQQS
                     TNNGNLRGYTARAVYTEPLWKRSLLELSAGTSYSKSTANRLTYDYNKNNGKHDQLNTL
                     LSNDFANTYSYTNGGFRLRTQRKKYNYALGLTWQQAELEGKIISGTKDSVISKTFRNF
                     LPNARFQYNFSRFRSLSLTYTTSTNQPTMTQLQPVPDISNPLNIRNGNPELKQEYNHM
                     LQTGLTLVSPYKNKNLFLFMTMQATSNKIVNYDSLNQQTGVRKTKPVNVSGVYNLNTT
                     ISYSLPVRFLKGSIELSSGAGYNKGKQLTNTLTGDMAVNTIKTLTMGPDVRLDMSPTP
                     KLNIGVGAGISYNNTKYSLQPGFSNNYLSQEFTTSLDWEMPKGFFFATDFNYTINSQR
                     AAG"
     gene            complement(2772..3362)
                     /locus_tag="PLO70_13695"
     CDS             complement(2772..3362)
                     /locus_tag="PLO70_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75569.1"
                     /translation="MKKTVKTLVLLTISLLFLERADAQLKLPSITGVGNDVKKVIEDY
                     PNRFINLMGEVMLQNAQSTDYHCNFKVNGAEETFITRYSAKREGVVSWQTLMLTTDDF
                     DEVKKKFRSFYNQLNNLPVSVGQKHFRLKGEYKLPQEEMKFTAVLFSLDPADEAAKKL
                     KVELSLQYQAPMEWRIKILVYDREREDDERGKAVEE"
     gene            complement(3718..>5940)
                     /locus_tag="PLO70_13700"
     CDS             complement(3718..>5940)
                     /locus_tag="PLO70_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75570.1"
                     /translation="TINIKLKKDKNKGVFGRALAAYGDNGRYEGNLSINKFKGDQRIS
                     LLFNANNINKQGFSFSDIISAMGGFGGFGGGGDRGGGGGGSFGGLQMTGGRGAAASFL
                     GGSNTAGIIRSLSTGINYTDIWASKIKVSGSYFFSNSNPLQEQSTFRQTFFTDSIALQ
                     DRTSISDNMNQNHRFNLRMEYQIDSNNSLIYIPSLTLQKSDNYNEDTTFVMADVPFGK
                     YLTSTSTARNTNERDGYNWGNNFLFRHKFKKTGRTLTLGWNNNLSNSNSEGFTYSNNR
                     FFRQDGSTFRTLLQNQQNTQETSSNNNVFSTSFTESFGLNKLLEINYAYTNNVSTSDK
                     ETYDFNTGSGKYDNLNLLLTNEFENTFLAHRVGANFRVQEKKYNYQLGLAVQQSTLES
                     QSYLASTGKDSVSRASYTNYFPTANFNFTPTKSKNLRFRYNGRTNQPSVNQLQNVLNV
                     TDPLNVSIGNPELEQEFTHNLNLSYNTFNILTFKFVAASINFTSTSNKIVNSIDTLTK
                     GVQLSKPVNLDGYSRIFSFVTLGIPFKNPKLKGSSLNFTNNISYIKDVSLLYKQKNIG
                     KTLMINQGVGMNYNKEKFDFGVKANLSYTNINYSVNKELNEQYYTQTYSADVSYIFKG
                     NFILSNDFDYYINTGRAEGFNQSIPLWNASLSKQLFKNKNGEIKFSVNDILNQNQSIS
                     RTNGDNYIQDTRSMVLRRYFMVSFLFNLNRMGGNNAPQMGMPGMPRSMQRQMGDMRMQ
                     "
     assembly_gap    5941..6040
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6041..6417)
                     /locus_tag="PLO70_13705"
     CDS             complement(<6041..6417)
                     /locus_tag="PLO70_13705"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQZ75571.1"
                     /translation="MKKIFTLSLLLFTLFITAQAQKADGSIKGKLVDSTTKQPISDAT
                     VSLLNAKDSSLATFTLSNKQGVFEIKGLAANSYQLIISHKSYDPISQFITISAAQKNI
                     DLGSLNPGTGGKSLENVTIISSVP"
     gene            complement(6531..6977)
                     /locus_tag="PLO70_13710"
     CDS             complement(6531..6977)
                     /locus_tag="PLO70_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75572.1"
                     /translation="MKIASIIFMLTFCLQADLFSQSIDSLRYKYTNQTIYRFGGVFQK
                     GNERLGFKDLSREFSMSDLGFDLYVKAKKQRKTSTILRYASLASSIAFLGVAANNRNR
                     NLAYGFLGGQMVFFMISMRYQGLSTQNLDQALWLRNKDVLFPDRRP"
     gene            complement(6989..7498)
                     /locus_tag="PLO70_13715"
     CDS             complement(6989..7498)
                     /locus_tag="PLO70_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75573.1"
                     /translation="MKNIGLFFILLIAGYSFRSNSRSSKIPLTIVYKFSIVPDSINDF
                     MTVYFQTKKIDVIKWEQVMTLFSEGIQAEMMSLTNSGKLNEKSAKDFIKNMPPVCNIL
                     AVTIFNDSTYSKNYLIDSIRWHVDVIPVKDTASRASNTFIPKPENKTNPYIVLKSFAD
                     NVIQSKLLK"
     gene            complement(7724..8269)
                     /locus_tag="PLO70_13720"
     CDS             complement(7724..8269)
                     /locus_tag="PLO70_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75574.1"
                     /translation="MSGAIKVIFFCFGLTFLLILYGWKKKDRFLSMPTIVLELKKENN
                     TEEYILKKDDQKEIKKYCKENKISVITKEDALSQYNKKNSEIMAPLFQTPADQLEEKK
                     KGIKREILSIPEIAKWITIKFYGSDTNSIDSSSLIINSFPYSEKNNIKINRSIIFETK
                     ISLLNCIKASIDTCIKNSYLK"
     gene            complement(8299..>8909)
                     /locus_tag="PLO70_13725"
     CDS             complement(8299..>8909)
                     /locus_tag="PLO70_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75575.1"
                     /translation="VLILLSFLVTTANAKSLPQTSNSTVLNFNYSLNSDVEKLVQDQE
                     FVEMINLIVDFSVNIYLGKKQDAVNRFAENSASEQDFKDILDAAKIKSKKELDNLFVN
                     IASLRQSVNKKYSFMENNQKAVNIFNEAVVTLVKNGKIKSIVLVDVDCLASFMAAFGL
                     CYWAYDECVINTGNSNNCAIALTACLSFVFISADQCFGIIGL"
BASE COUNT         2516 a         1774 c         1628 g         2891 t
ORIGIN      
        1 accctgccgc ccgctggctg ttgatcgtat aattaaaatc ggtagcaaag aagaaacctt
       61 tgggcatttc ccaatccagc gatgtagtaa attcctgtga aagataattg ttgctgaagc
      121 ctggttgcag agagtatttc gtattgttat aactgatacc ggcgcccacc ccgatattca
      181 gttttggtgt agggctcata tccagccgca catcaggtcc catcgtcagt gtcttaattg
      241 tatttaccgc catatccccg gtcaatgtat tagtgagttg ttttcctttg ttataaccag
      301 ccccgctgct cagttctata cttcccttta aaaaacgaac cggtaaactg tagctgatag
      361 tagtgttcag gttatataca ccacttacat ttaccggctt tgtctttctc actcccgttt
      421 gctggtttaa tgaatcatag ttgacgatct tgttggaagt agcctgcatg gtcataaaca
      481 aaaacaggtt tttattctta taaggactta ccagtgtcaa tcctgtttgc agcatatgat
      541 tatattcctg cttgagctca ggattcccgt tccggatatt taacgggttg ctgatatccg
      601 gtaccggttg cagctgtgtc atcgttggct gattggttga tgtagtatag gtaagcgaca
      661 aacttctgaa ccgggagaag ttgtattgaa accttgcatt gggcaaaaag tttcggaagg
      721 ttttactgat cacagaatct ttggtaccgc tgatgatctt tccttccagc tccgcctgtt
      781 gccaggtaag ccccagtgca taattgtatt ttttcctttg tgtacgaaga cgaaaaccac
      841 cattggtata gctgtatgta tttgcaaaat cattactgag taatgtattc agctgatcat
      901 gcttaccatt attcttattg taatcgtagg tcagcctgtt tgctgtactc ttactgtaac
      961 ttgttcccgc actcagttcc agtaaagatc gtttccagag aggctctgtg taaacagccc
     1021 tggcagtata acctctcagg ttgccgttat tggtgctttg ctgattgagg gtatctttcc
     1081 gcaataatga accgccggga gcataaaaac tattgacaga tgaaagactg ccatcccctt
     1141 cactttcatt caggctggtt tgcagcgaca gggaaaatgt tcttccctta cgggcaaatt
     1201 ttctgcgcca gataatatca ttacgaaaat tatatccttc actgttagag agattattgc
     1261 tgaaaccctc attgatcaac ccctgctgtt cggataaggt ctggtaatca ctctgtgacc
     1321 ggttacttgt tttttggtaa ctgaaagagg gagtgatacg tatggaattc atggaatcaa
     1381 gctggtacag catgttcagg ttaagccggt gtgtattgtt caggttgtct gaataggtat
     1441 tggcgttata ataagaggta tccggtaaaa tatattgccg ctgaatgtga ctctctgtat
     1501 tcgggttata gcggttataa aagtaattgc tctgaaaatc gagtttggta ccaatgatat
     1561 tattatagtt cagtccgcca ccccaggcgg tattgatacc gctgttgcgg ttaccactat
     1621 tcatgcccat cgcagcggca tcctggctgg aaatattgat attgatatta ccaccgccac
     1681 cacgttgcat gcgggccatt tcaccggtaa aattcaatat atccataaaa gaaaatcctt
     1741 cagcattggt attattgccc ataccgatgg cagagaattg cctggctcct ttaaatgaat
     1801 tgacattgaa tttcccctcg tagcgttctt tattccctgc tcctgcattc actttcccaa
     1861 acaatccctt ctttttatct ttttttagtt tcaggttgat ggtcttttca taattaccat
     1921 cctcaaatcc ggtcagttgc gcctggtcac tttgcttatc atacacctgt actttgtcca
     1981 ccgcatctgc cggcaggttt ttcgtggcga tcttggggtc gttcccaaaa aactcttttc
     2041 catctaccag cacccggctc actgtttctc cctgtgcttt gatggtgcca tccttctcca
     2101 cttttacacc cggcaatttt ttcagcaatt gttccacact ggcattaggc tgaactttaa
     2161 aagatccggc attatactga atggtgtcat tgatcagcgt aaccggtggt gcttcattgg
     2221 ccagcactac ctcttccagt actttggctt tatcattcat aaccaggttc cccatatcgg
     2281 cattcttatg ggcatcagaa attgaaaaat atttattgct gttatggtaa ttcacatgag
     2341 tgatcagcaa ccggtaatca ccattcgcaa gaccttttag ttcaaaccgt ccatcactac
     2401 ctgtcatggt aaatgttacc agcgaagaat cttttctttc aagtacagta atagtggcgg
     2461 cgctaacagg ttgtttgctg atggtatcaa aagccagccc ttttacagcg ccattcttct
     2521 gtgccagcaa tagggtggca acaaaaaaag taagtactaa aagataaatt ttcctcatag
     2581 ccattttgtt tcagctacga aaatattctg actatcccgg aatagtggtt aaaagggctt
     2641 ggtttattgt taatgaaggt taatgtttcc taagccacag cattttgata gtgagcggga
     2701 tgagcagctg tcttattaaa agtacagaat aaaattccgg ctcatgcccg gaatgacttc
     2761 gtttgcaaag actattcttc cacggccttt ccccgctcat catcctccct ttccctgtca
     2821 tacaccagta tttttattct ccattccatg ggtgcctggt attgcagaga cagttccact
     2881 ttcaattttt tagcggcttc atcagcgggg tccaatgaaa aaagtaccgc ggtgaatttc
     2941 atttcttcct gcggcaactt atactctcct ttcaggcgaa aatgtttttg accaacgctt
     3001 accggaagat tgttgagctg attgtagaat gacctgaatt tcttttttac ctcatcaaag
     3061 tcatcggtgg taagcatcaa tgtctgccag ctcaccactc cttctctttt ggctgaatac
     3121 cgggtaataa atgtttcttc tgcaccattc actttaaaat tgcaatggta atcggttgac
     3181 tgtgcatttt gaagcatcac ttcacccatg aggttgataa agcggttagg ataatcttca
     3241 attactttct taacatcatt ccctactccg gttatggaag gaagttttag ttgtgcatct
     3301 gccctttcaa gaaagagtag tgaaatagta agaagaacaa gtgtttttac tgtttttttc
     3361 atttgtacgg gatttaacgg gttgataaca agccccaata ttataaaatt ttaattttat
     3421 ttcgtacttt tagtaacaac ccgttcgtta acccttcgta aaatatccag cgtatgaaaa
     3481 acaaacatct tgtacatcat catgaatcgg cgaaaatcta tcgtcattac tacaaaccct
     3541 cattcagtat tgtgcggttg ataaaatctg ttttagcagt tataatatcc ggtacatcac
     3601 cggcagcagc caatcattac aggaggcatt gatcattttt tttactaacc cttactatac
     3661 taaaaaaggg ctcactgatg gtgaagccct ttttttattg aacaataagt taagtcctta
     3721 ctgcattcgc atatcaccca tctgccgttg catggatctt ggcatgcctg gcatacccat
     3781 ttgcggggca ttattaccac ccattctatt caggttaaac aaaaaactaa ccataaaata
     3841 acggcgtaac accatactgc gggtatcctg tatatagtta tcgccattgg tacggctgat
     3901 actctggttc tgattcagga tatcattcac agaaaattta atctctccat ttttattctt
     3961 aaataattgc ttgctcaggc tcgcattcca aagcggtata ctttggttaa acccttcagc
     4021 acggcctgta ttgatataat agtcaaaatc attcgataaa atgaagttcc ctttaaaaat
     4081 ataagagaca tcagcagaat acgtttgtgt ataatattgc tcgttcagct ctttattcac
     4141 cgagtaatta atattggtat aggacaagtt ggctttcacg ccaaagtcaa atttttcttt
     4201 attgtagttc atccctacac cctggtttat catcaaagtt ttaccaatat ttttttgctt
     4261 atacaacaaa ctcacatcct tgatatagga aatgttattg gtaaaattca ggctggaacc
     4321 tttgagtttt ggatttttaa acggtatccc taatgtaaca aaggagaata tccttgaata
     4381 accatctaaa ttgacaggct tgcttaactg aacccctttg gttaaggtgt caatactgtt
     4441 aacgatctta ttactggttg aagtaaaatt gatactggct gcaacaaatt taaacgtaag
     4501 gatattaaag gtattatagc tcaggttgag gttatgtgta aactcctgtt ccaattccgg
     4561 gtttccgata gatacattca gtggatctgt tacattcaaa acgttttgca gctggttgac
     4621 agaaggctga ttggttctgc cattatacct gaaacggaga tttttacttt ttgtaggtgt
     4681 aaagttgaaa ttagcagtag gaaaataatt cgtatagctg gcccggctta cagaatcttt
     4741 accggtactg gcaagataac tttgactttc cagggtggat tgctgtacag ccaatcccag
     4801 ctggtaatta tatttctttt cctgcacccg aaaattggca ccaacccggt gtgccaggaa
     4861 agtattttca aattcattgg tgagtaatag attcagatta tcgtatttac ccgaacctgt
     4921 attaaaatca taggtttctt tatcagaggt acttacatta ttggtatagg cgtagttaat
     4981 ttccagtaat ttgttcaacc cgaatgattc ggtgaaagag gtgctgaaca cattgttatt
     5041 gctactggtt tcttgtgtgt tctgctgatt ttgcagcagc gttctgaaag ttgaaccatc
     5101 ctgcctgaaa aacctattat tggagtacgt aaatccttcg ctattgctgt ttgataaatt
     5161 attattccag cccagggtaa gcgtacggcc ggttttctta aacttatgcc tgaataaaaa
     5221 attattaccc cagttatagc cgtctctctc attggtattt cttgccgtac tggtggaggt
     5281 aagatattta ccgaaaggaa catctgccat tacaaacgtg gtatcctcat tatagttatc
     5341 cgacttttga agggttaagg atggaatata tatcagggaa ttgttggaat cgatctggta
     5401 ttccatgcgc aaattgaacc ggtgattctg gttcatattg tctgaaatac tggtacggtc
     5461 ctgcaaggcg atggaatcag taaaaaaggt ttgccggaat gttgactgtt cctgtaacgg
     5521 attactgttg gagaaaaagt aactgccact tactttgatc ttacttgccc agatatccgt
     5581 ataattaata cccgtggaaa gggacctgat aataccagcc gtattcgaac cacccaaaaa
     5641 acttgccgca gctcccctgc cacctgtcat ttgcaggcct ccaaaacttc cgccgccacc
     5701 gccgccccga tcaccgccac cgccaaatcc accaaaccct cccatggcac tgatgatatc
     5761 agaaaaggag aatccctgtt tatttatatt attggcgttg aataaaagtg agatccgctg
     5821 atcgcctttg aatttattga tcgataaatt tccttcgtac cggccattat ccccataagc
     5881 agcaagagcc cttccaaaca ctcctttatt cttatccttt ttcaatttaa tattgatcgt
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtacgctgg aaataatggt
     6061 aacattttca agcgatttac caccggtacc ggggtttaaa cttcccaggt caatattttt
     6121 ttgcgctgct gaaatagtga tgaactggct gataggatca taacttttat gactgatgat
     6181 cagctggtag ctgtttgctg ccagtccttt tatttcgaat accccctgct tgttggacaa
     6241 ggtaaaagtt gccagggatg aatctttggc atttaataag gaaacggtag catcagaaat
     6301 gggttgtttg gtagtactat ccaccagctt tcctttaatg ctcccatcgg ccttttgggc
     6361 ctgtgcagta ataaataggg taaatagaag gagggataag gtaaaaatct tcttcataat
     6421 cagctaaata agtgtacgaa aatagacgta ataaccggaa acaactttcg ggaagttgtt
     6481 cttttttcca aaaagaggga taaacaggcc gattttaccg atgaatgggc ttagggccgc
     6541 ctgtcgggaa atagtacatc tttatttcgc agccacaggg cctggtcaag gttttgggta
     6601 ctaagcccct ggtaacgcat actgatcata aagaaaacca tctggccgcc aagaaagcca
     6661 taggcaaggt tcctgtttct gttattggct gccaccccaa gaaaggcaat actagaagcc
     6721 aaagaagcat agcggagtat cgtacttgtt tttctttgct tttttgcttt tacatacaaa
     6781 tcgaagccaa gatctgacat agaaaactcc ctgctcagat ctttaaaacc cagccgttca
     6841 tttccttttt gaaaaacacc tccgaaacga taaatagttt ggttggtgta tttataacgg
     6901 agggagtcaa tggattgaga aaataaatct gcctgcagac aaaaagttaa catgaaaatg
     6961 atagaagcaa tcttcataat gattcttatt atttaagcaa tttggattga atcacattgt
     7021 cggcaaaaga tttaagaacg atgtaaggat tagttttatt ttccggcttt ggtataaacg
     7081 tgttggatgc tctactggca gtatctttta caggtatcac atccacatgc cagcggatac
     7141 tgtcaatcaa ataatttttg gaataggttg agtcattgaa aatggttact gccagtatat
     7201 tacaaaccgg cggcatattt tttataaaat cctttgctga tttttcattt agtttgcctg
     7261 aatttgttaa tgacatcatt tctgcctgta ttccttcgct aaaaagtgtc attacctgct
     7321 cccatttaat aacatcaatt ttttttgttt gaaaatacac cgtcataaaa tcattaatac
     7381 tatcaggcac aatcgaaaat ttgtaaacaa ttgtaagggg aatttttgaa gagcgggaat
     7441 tactcctgaa tgaatatcct gctatcagaa gaataaaaaa taacccgata tttttcatac
     7501 gctataagtt ttaataaagc aacctgtcac ttataaaatc tgacaggtta ctttatttaa
     7561 aaattttaat ctgccgtcaa aatacatatt aaatccaata gagcccatcc gatttaataa
     7621 tcctgtgttg tattttatat atatatcgaa tcttgagttt gttaatagcc aaatgcggat
     7681 aggaagaagg taaccaacaa aagggatatt ctaaacaatg tattcatttt aagtagctat
     7741 tttttataca agtatctatt gatgctttaa tacaattaag taatgaaatc tttgtttcga
     7801 aaattatact cctgttaatt tttatattat tcttttcaga gtacggaaag gaatttataa
     7861 ttaaagaaga agaatcaata gagtttgtat cagaaccgta aaattttatt gtaatccact
     7921 ttgctatttc cgggattgac aatatctctc ttttaattcc ctttttcttt tcctcaagtt
     7981 ggtctgccgg cgtttgaaaa agtggagcca ttatttctga attctttttg ttatactggc
     8041 ttaatgcatc ttcttttgtt attacgctta ttttattttc tttacaatat tttttaatct
     8101 ctttttgatc gtcctttttt aatatatatt cttcagtatt attctctttt tttaactcta
     8161 aaactattgt tggcattgat aaaaatctgt ccttcttttt ccaaccgtat aagattaaca
     8221 aaaacgttaa gccaaaacaa aaaaatatta ctttaatagc cccactcata aactagattc
     8281 taatatcagg taaaaaaatt atagcccgat tatgccaaag cattgatcag cactaataaa
     8341 gacaaatgat aaacaggccg taagggcaat tgcgcaatta tttgagtttc cagtattaat
     8401 tacacactca tcataagccc aataacacaa accaaatgca gccataaaag aagcaagaca
     8461 atcaacatca accagtacta tactttttat tttcccattt tttaccaatg taacaactgc
     8521 ttcattaaaa atattcacag ccttctgatt gttttccata aaggaatact ttttattaac
     8581 actttgcctc aggctagcga tattaacaaa caaattgtct aattcttttt tgcttttgat
     8641 ttttgcagca tcaagaatat ctttaaaatc ttgttcagat gcagagtttt cggcaaaccg
     8701 attaactgca tcttgctttt tacctaaata aatattcact gagaaatcaa caatcagatt
     8761 aatcatttca acaaattcct gatcttgtac taatttttca acatcggaat ttaaagagta
     8821 attaaaatta agaacggttg aattggaagt ttgtggaagg cttttggcat tagccgtggt
     8881 aacaaggaaa gagagtagta ttaataccc
//
LOCUS       DAORTI010000134         8869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8581_length_8869_cov_5.444180, whole genome shotgun sequence.
ACCESSION   DAORTI010000134 DAORTI010000000
VERSION     DAORTI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_8869_cov_5.444180"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..810
                     /locus_tag="PLO70_13730"
     CDS             166..810
                     /locus_tag="PLO70_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75576.1"
                     /translation="MPIAYEKGPVHVSIDDNSYPYVTVSAWQVFGKNIRQVHEIPCKI
                     PDNNAPKAARKFIDWLLRIGHNDVVFVYGDPSSKKASTVDENSKSFYDKFFEELNKTR
                     FKMQNRVIKSAPEVALSAAFINEIYEVGINGYNITISDSCKVSIEDYLTVKQAPDGTM
                     LKPKEKNTLTGKMYETHGHFSDAKRYFIIELLKEEWNKYKSRKNNLASQAGWFR"
     gene            810..1223
                     /locus_tag="PLO70_13735"
     CDS             810..1223
                     /locus_tag="PLO70_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75577.1"
                     /translation="MNYSKNILANIGRVVVSKHPDIAQSIINQVEENIGIQKEAIQFM
                     FERYCKMMGIKDSELTGPVFKSRFTEIRKIFISVIISFYGDRRRLNKILSDILQQPSS
                     LMSRQISEVKFRYKKDADFMDKCNKAIQCLQKPIN"
     gene            1230..2696
                     /locus_tag="PLO70_13740"
     CDS             1230..2696
                     /locus_tag="PLO70_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HQZ75578.1"
                     /translation="MELSVIISLIERNDIQKLISCMDSYCGTNAYKESIKKYEVKNHD
                     VMDKAKRPDKLVKGEDQEGDTIVQVARIPIPEQKRIVKLSAAFMGVPALNGTAENDAE
                     KNMLTIINAINSDNKMEYNFRSILKMTMSETECAELWYPVETEEGYYDGLPMKSKYKL
                     RVRLLAPSLGDALYPIFDKFNDLIAFGRGYVIKTHEEDGREKDIERLDVYTADKIYYL
                     QKEGQGDWAPVKIQKTGDEKVSGSGIVNIFKKIPVVYYSQPSPEWQDVENLIDRLEKK
                     VSNHADTNDYFDSPIVLAKGDVKGFTKKGEQGKVLELGSDADVSYLTWDSLPESKKME
                     DENLNRAIAKYTHTPDISLDNLKSIGALSGIALRTFFMDAHLKASDKEEIFGIGVTRR
                     INFLKSAVAILDSRLKQGKRVNIKPVFTYFLPKDIQGEITSIVSAVNAGIISKESAVK
                     INPLVEDWESELEKINQEEEKKTKQQQDNIVLPGIKHE"
     gene            2802..3449
                     /locus_tag="PLO70_13745"
     CDS             2802..3449
                     /locus_tag="PLO70_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75579.1"
                     /translation="MKEKIIASIKAKFPAVNLSKARLDAIAAILEKKVTDENEIDAKL
                     DEFNDYNPFADIAKQDDAMRNMQNKLKTAGQQNPTKKDSNHNQEDGGDPKDDDDDTPP
                     WAKALMKSNNVLLEKVQQLEGEKTKSSIQKAIADKLKEIPGNYWNKRAIPQKEDDIDQ
                     FVIDVQADYADFQKELVEQGIAVHPAPVGGSGSGRGKGEKAASKEEIDAVVNEIM"
     gene            3460..4125
                     /locus_tag="PLO70_13750"
     CDS             3460..4125
                     /locus_tag="PLO70_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75580.1"
                     /translation="MAAIVNLNDDVTEVVTGNDSIVIVDNFQSIRGGRALDTTGFSPK
                     VINAGHVIIKETSTGAHKPMPATEASIGNIATLGSVTAGTTYTNGTYENVPLSGGTGS
                     GALATVVVAGTVVTTVTITKAGKDYTVADVLTADNTYIGGTGSGFSVPVATVTTTAAA
                     YGSLPSGHTYVGILIASILTAKPFAGIMVRGTVNPAAAPFSMTSILSAFKTAVPLIDF
                     RED"
     gene            4132..5217
                     /locus_tag="PLO70_13755"
     CDS             4132..5217
                     /locus_tag="PLO70_13755"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HQZ75581.1"
                     /translation="MKQTSLFVQYVSKFFSGVVAAIVNKINDSKNPLVYYHKRFLKKQ
                     YSPDGKWESITAANTLVMADVVAMDSSLPLKKRDSISRASGDIPKMGMEMALREKQLT
                     DLRTLALRPETEAQLITRIFNDVPRVIGGVYERNEAIFLEALSTGVAEITDTENVGTS
                     IRLDYGYLSANQSGVATVWSSTSATPLDDLQGVWNNAKNAGNIIQKWLMDRATFNNFA
                     KTTQVKEYYAFSVGFAGTASQIPSLSKVNEFLKDTFGFTIEIIERSVRYEKNGVQTVA
                     TPWATGIVIGICSEELGLLQWARLAEADFPVEGVTYQTADEYILVSKYRSNQPSLAEW
                     TSSQSRVVPVLTNVDQIYKIDSTEVEG"
     gene            5227..5430
                     /locus_tag="PLO70_13760"
     CDS             5227..5430
                     /locus_tag="PLO70_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75582.1"
                     /translation="MAKVEITKPEETLEVEKATDKKKKYIVVAPFRDKTNFNKKFQAG
                     EDVSSFDKGRLDYLLGKKLIELK"
     gene            5427..5759
                     /locus_tag="PLO70_13765"
     CDS             5427..5759
                     /locus_tag="PLO70_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75583.1"
                     /translation="MTNLERLISLIGFAPSNNDSLQGALIDFGINGADTYTVANKLVV
                     KKAAIQVIQLLLSTADTNNGMSNGAGFEIKYDRQSLLDYLDRLKKEVEEEENGVESNR
                     LITGKTVW"
     gene            5760..6656
                     /locus_tag="PLO70_13770"
     CDS             5760..6656
                     /locus_tag="PLO70_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75584.1"
                     /translation="MVHPYLPDIIKGIVERINNVFSTRETDPFTVLFTHGNYAQANRD
                     FYKAPQEGKNNLIWLKMPFDQVVGKSSLYYSDLVFSIAIATVSDNKLSQTERDNTTIK
                     PRLYPIYDLLMQEIDREGWFVYNRTRTPEHRAIVESYWGIGDVNGQDADNLFKIKADV
                     MSVLDLKIKLRKSSCRSQYNPLELTDVPYQPVQLTFFKDIELIVDGGADIDPADGADT
                     VLIPDLAGFEYDVVQRAFGQLRKERAPEIVINAAGGFTLTGGYKFSKDDTYIIKLRPT
                     FTEVSELPGVLQKNIAKVGIEI"
     gene            6686..7636
                     /locus_tag="PLO70_13775"
     CDS             6686..7636
                     /locus_tag="PLO70_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75585.1"
                     /translation="MAYSLCGSSVDNTGEQECDKSRGVGRKLFITAAAIAPADFATKE
                     AFMAKLVANSKLSKNHPDKVFPINELQDIADNSEANKEGSLNLGFSAVLLEGKPKYTF
                     KVFAGSDLLKRYRSFNNQTVRIIEYDANGVFWVTKVGANAKGFQFKLFTTGNKLATGQ
                     AVEEGVVTITASVMSNTEYLDNCFWVESNGNVEDIKALIDVQLAYVSNASNVYKYSAK
                     IPGSNLTGDYSILPTYGSAIAALVADFSAKSGAGVPATSLAITSISYDSTLDALTVTY
                     DSTAYTSATGNIMLVPPTPAQLDTGSVPDSELLSVTHPKA"
     gene            7710..7898
                     /locus_tag="PLO70_13780"
     CDS             7710..7898
                     /locus_tag="PLO70_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75586.1"
                     /translation="MTFESVTFNAAYWKDKKEHEFIKHESHHGLSVEQLKEAFAVIKG
                     NTATPEKKKEDADFKGSL"
     gene            7873..8310
                     /locus_tag="PLO70_13785"
     CDS             7873..8310
                     /locus_tag="PLO70_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75587.1"
                     /translation="MPTLKGLYDKIINMDVNKIIDRSIDNTVDAMADINRERMLSGVD
                     ADGKVMPFYSKRSQEEFGKPNGPHILKDTGAFQSKLFSRRRGENIETSSTNEKADMLE
                     KRYGHADIFIFGLSGDFKKKYQDEYLAPEIKVHISAETGLKFK"
     gene            8398..>8869
                     /locus_tag="PLO70_13790"
     CDS             8398..>8869
                     /locus_tag="PLO70_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75588.1"
                     /translation="MKRNKLWQLLKKDQSSKYSRQQQSHPVASMLNLYQSISELPLSR
                     FIDAVVDNNLFSLIKSGNAQPDMLQLSAAWKNILDEYTEAMGSHEYKLYISLLREKEQ
                     LIIDYNLIRSLVDAMHNVQDYIISEGFVVNDDVINFQKESGKSLSEVLRYNFIFN"
BASE COUNT         3025 a         1527 c         1934 g         2383 t
ORIGIN      
        1 tcacccgtcc ggtaagggtg ggttttatga cacagaaacc attcgtgatt tgaaagggat
       61 aggcagcgaa atagcattaa ctacagggtt tacgctaatg gggagagagg gatatttaaa
      121 acaggcggtg agttttggat acagtttgag ccggacaaac atgcgatgcc gattgcttac
      181 gaaaagggtc ccgttcatgt atctattgat gataatagtt acccgtatgt taccgtgtcc
      241 gcctggcagg tgttcgggaa aaacataagg caggttcacg aaataccttg caaaatccct
      301 gacaataatg ctcctaaagc ggcgagaaaa tttattgatt ggcttcttag aatcggtcac
      361 aatgatgttg tattcgttta tggagaccca tcatcaaaga aagcatctac tgttgatgaa
      421 aatagtaaat ctttttatga taaatttttt gaagagctaa ataaaacgag gtttaaaatg
      481 caaaacaggg tcataaaatc agccccggaa gtagcgctga gtgcagcctt tattaatgag
      541 atatatgaag ttggtatcaa cggatacaat ataactataa gcgattcatg caaagtgagc
      601 attgaagatt acttaaccgt aaagcaggcg ccggatggga caatgttaaa gccaaaggaa
      661 aaaaacacat taaccggtaa gatgtatgaa actcacgggc atttcagcga tgctaagcgt
      721 tactttatta tagagctttt aaaagaagaa tggaataaat ataaatcaag aaaaaataac
      781 cttgcttcac aagcggggtg gtttaggtaa tgaattattc aaaaaatatt ttagccaata
      841 taggcagagt tgttgtatcg aagcaccctg atatagccca atcaataatt aaccaggtgg
      901 aagaaaatat aggcattcag aaagaggcta ttcagtttat gtttgaaagg tattgcaaaa
      961 tgatgggtat taaagatagc gaactaacag ggcccgtttt taaatcaaga ttcaccgaaa
     1021 taagaaaaat ctttatcagt gttattatat ccttttatgg cgaccgaagg aggcttaata
     1081 agattttatc tgacatacta cagcagccat cttctttaat gtcaaggcaa atttcagaag
     1141 tgaaattcag atataaaaaa gatgctgatt ttatggataa atgcaacaag gcaatacaat
     1201 gtttacaaaa accaattaac taaaaactta tggaactgag tgtaattatt tctcttatag
     1261 aaagaaacga catacaaaag ctgataagct gcatggattc ttattgcggc acaaatgcat
     1321 ataaagagtc tattaaaaaa tatgaagtaa agaaccatga tgtaatggat aaagccaagc
     1381 ggcctgataa attagtgaaa ggggaagatc aggaaggaga taccattgta caggttgcca
     1441 ggatacctat acctgaacaa aaacgcatag taaaattatc tgccgctttt atgggtgtac
     1501 cggcgttgaa tggtacggct gaaaatgatg cagaaaaaaa catgctcacg attataaatg
     1561 caatcaacag cgataataaa atggagtata atttccgttc tattctaaaa atgacaatga
     1621 gtgaaactga atgtgcggaa ttgtggtacc cggtagaaac agaggaaggt tattatgatg
     1681 gtttgccaat gaaatcaaag tataagttaa gggtaaggtt gctggctccg tcattgggag
     1741 atgctttgta cccaattttt gacaaattca atgatttgat tgcttttgga agagggtatg
     1801 ttattaaaac acatgaagaa gatggaaggg aaaaagatat tgaaaggctt gatgtttaca
     1861 ctgccgataa aatatactac ctgcaaaaag aaggacaggg ggattgggcc ccggttaaaa
     1921 tacagaagac aggtgacgaa aaggtttcag ggtcaggcat tgtaaatatc tttaaaaaaa
     1981 tacctgtagt ttattacagc cagccttcgc ctgaatggca ggatgtagaa aatcttattg
     2041 accggttaga aaagaaggta agcaatcatg ccgataccaa tgattatttc gacagcccta
     2101 ttgtacttgc aaagggagat gtgaaaggat ttactaaaaa aggagagcaa ggcaaggtac
     2161 tggaattagg gtctgatgca gatgtatcat accttacctg ggattcttta ccggagtcta
     2221 aaaaaatgga agatgaaaat ctaaacaggg ccattgcaaa atatacacac actccagaca
     2281 tcagccttga taaccttaaa tctatcggcg ctttatccgg catagcgctg cgcacatttt
     2341 ttatggatgc ccatttaaag gcatcggata aggaggaaat ttttggcata ggtgttacaa
     2401 ggcgtattaa ttttttaaaa tcagccgtgg ccattctcga ttcaagattg aagcaaggta
     2461 agagggttaa tataaaaccg gtgtttacct attttcttcc aaaggatatt caaggtgaaa
     2521 ttacttctat tgtatctgct gttaatgcag gtattatatc aaaagaatca gcagtaaaga
     2581 tcaatcctct tgttgaagac tgggagagtg aattagaaaa aataaaccag gaggaagaaa
     2641 agaaaacgaa acaacagcag gataatattg ttttacccgg tattaaacat gagtagccaa
     2701 tttgttagca aaaaatgttt gctatctttt ttcacgtcat aatttatttc cattcatgat
     2761 ttttgtttca gttttatgat tgaaacaaaa agctcttata catgaaggaa aagattatcg
     2821 cctcaattaa ggcaaaattc ccggctgtaa atctttctaa agcaaggctg gacgcaatag
     2881 cggctatact tgaaaagaaa gttacagacg aaaatgaaat tgatgcaaag ctggacgagt
     2941 tcaacgatta caatccgttt gcagacattg caaaacaaga tgatgcaatg cgtaacatgc
     3001 agaataaatt aaaaactgcc ggacagcaaa atcccacaaa aaaagattcc aatcacaacc
     3061 aggaagacgg tggtgaccca aaggatgatg atgatgacac ccccccttgg gcaaaagcat
     3121 taatgaaaag caataatgta ttgcttgaaa aagtgcagca gctcgaaggg gaaaaaacaa
     3181 aatcatccat ccaaaaggcc attgctgata aactgaaaga aattcccggt aattattgga
     3241 ataagagagc cattcctcaa aaggaagatg acatagatca gtttgtaata gacgttcagg
     3301 ctgactatgc agattttcaa aaagaattgg ttgagcaggg gatagcagtg catccagcac
     3361 ctgtgggcgg tagtggttcc ggaagaggta agggagaaaa ggcagcatca aaagaagaaa
     3421 ttgatgctgt cgtaaatgaa ataatgtaaa attttaaaaa tggcagctat cgtaaatctc
     3481 aatgatgatg tgacagaagt tgttaccggt aacgattcta tcgttattgt agataacttt
     3541 cagtcaatcc gtgggggacg tgccttagat actaccgggt tttcgccaaa ggtgataaat
     3601 gccggtcatg taatcataaa ggaaacatct accggcgccc acaaaccaat gccggctaca
     3661 gaagcaagca ttggaaatat tgccacactt ggatctgtaa cagcaggaac tacatacaca
     3721 aatggcacct acgagaatgt accgctttcc ggtggcaccg gttccggcgc acttgcaacg
     3781 gtagtggtag caggtacagt agttactact gttactatca ctaaggccgg caaggactat
     3841 accgttgccg atgttcttac cgctgataat acatatatag gtggcaccgg gtcaggtttc
     3901 agcgtaccgg tagcaacagt taccactact gctgccgcct atggttcgct tccttcaggc
     3961 catacttatg taggtattct tattgcatca atattaacag ctaaaccctt cgctggtata
     4021 atggtaaggg gaaccgttaa cccggccgct gcgcctttct caatgacaag cattttgtca
     4081 gcatttaaaa cagcagtacc actaatcgac ttcagggagg actaataaat tatgaaacag
     4141 acatccttat tcgttcagta tgtatcgaag ttctttagcg gtgttgttgc tgctatagta
     4201 aacaagataa acgacagtaa aaacccgttg gtttattacc ataaaaggtt tttgaaaaaa
     4261 caatattcac ccgatggtaa atgggaaagc attacagcag ccaatacatt ggtaatggcc
     4321 gatgttgttg ccatggattc atctcttcca ttaaagaaga gggattctat ttcccgtgca
     4381 tccggtgaca tacctaaaat gggtatggaa atggcgctgc gtgaaaaaca gctaaccgat
     4441 ctgcgcaccc tggcacttcg cccagaaaca gaagcacagc tcattacaag gatttttaat
     4501 gatgtacccc gggtaatagg tggcgtttat gaaaggaatg aagctatatt ccttgaagcc
     4561 ctttctaccg gcgtggcaga aattacagac acagaaaacg ttggtacatc tatcaggctg
     4621 gattacggtt atttatccgc aaaccagtca ggcgttgcca ctgtatggtc aagtacatcg
     4681 gctacaccat tagatgattt gcagggagtt tggaataatg caaaaaatgc cggcaacatt
     4741 attcagaaat ggttgatgga ccgggccact ttcaacaatt ttgccaaaac aacccaggta
     4801 aaagaatatt atgcttttag cgttggtttt gccggcacag cctcacagat accttctctt
     4861 tcaaaagtga acgaattcct gaaagacact tttggtttta ccatcgaaat cattgaacgc
     4921 tctgtacgct atgagaaaaa tggtgttcag accgttgcca ctccatgggc tactggaatt
     4981 gtcataggta tttgtagcga agaattggga ttgcttcaat gggcaagatt ggccgaagct
     5041 gacttccctg ttgaaggtgt aacataccaa acagcagatg agtatatcct tgtatcaaaa
     5101 tacagatcaa accagccgtc attggcagaa tggacaagct cacaatcacg tgtggttcct
     5161 gtgttgacca acgttgatca aatttataaa attgattcta cagaagtaga aggataattt
     5221 ttttttatgg caaaagtaga aataacaaaa ccggaagaaa ctttagaagt agaaaaagca
     5281 acagataaaa agaaaaaata tatcgttgtt gctcctttca gggataagac aaacttcaat
     5341 aaaaagtttc aggccggtga agatgtaagc tcatttgaca aaggcaggct tgactattta
     5401 ctgggtaaga agctcattga attgaaatga ctaatctcga aagattaata tcattaatag
     5461 gttttgcccc ttcaaataat gattctctac agggtgcatt aattgatttt ggtattaacg
     5521 gggccgatac ttatacagta gccaacaagc ttgttgttaa gaaagcagca atacaggtta
     5581 tacaattact gttaagcaca gctgatacaa acaacggtat gagcaacgga gcaggttttg
     5641 aaatcaaata tgacaggcag tcccttctcg attacctcga caggcttaaa aaagaagttg
     5701 aagaagaaga gaatggcgtt gagtcaaaca gacttattac aggtaaaaca gtatggtaaa
     5761 tggtacatcc atatctgccg gatattataa aagggattgt tgagcgtatt aacaatgtat
     5821 tctcaacgag ggagacagat ccttttacag ttctttttac tcacgggaat tatgctcagg
     5881 caaacaggga tttttataaa gcgccgcaag aaggtaaaaa caatcttatt tggttgaaaa
     5941 tgccctttga ccaggtagtt ggtaaaagtt cactgtatta ttctgatctg gttttttcta
     6001 ttgcgatagc tacagtatca gacaataagc tatcgcagac agaaagggac aacacaacca
     6061 ttaagccaag gctttatcca atatatgatc tgctgatgca ggaaatagac agggaaggtt
     6121 ggtttgttta taatcgaaca agaacgcctg agcacagggc aattgtggag tcttattggg
     6181 gaattggtga tgtaaatggg caagatgctg ataacctttt taagataaaa gcagatgtta
     6241 tgtctgtatt agacttaaaa ataaaactaa ggaaatcatc ttgccggtca caatacaacc
     6301 cattagaatt aacagatgtt ccttatcaac cagtgcagct tactttcttt aaagacatag
     6361 agctgatagt tgatggtggc gctgatattg atccggcaga tggtgcggac actgttttaa
     6421 tacctgattt ggcaggtttt gaatatgatg tagtgcagag ggcttttggg cagttaagga
     6481 aagaaagggc cccggagata gtaatcaatg ctgcaggtgg gttcacgtta accggtggtt
     6541 ataaattcag taaggatgat acctacatta taaaactaag gccaacattt actgaagtaa
     6601 gcgagttgcc gggtgtattg caaaagaata ttgcaaaagt tggaattgaa atttaaaatt
     6661 tttaaacaaa ataaaattaa aaaacatggc ttattcatta tgtggttctt cagtagataa
     6721 tacaggcgaa caggaatgcg acaagtccag gggtgtaggc agaaaattat ttattactgc
     6781 tgctgcaatt gcgcctgcag actttgcaac aaaagaagcg tttatggcaa aattggttgc
     6841 caattcaaag cttagtaaaa accatcctga taaggtattt cccataaacg aattgcagga
     6901 catagcagat aattcagaag ccaataaaga aggctcatta aaccttgggt tttcggctgt
     6961 gttgctggaa ggaaaaccaa aatacacttt caaagtattt gccgggtccg atcttttgaa
     7021 aagatacagg agtttcaata atcaaaccgt gcggattatt gagtatgatg caaatggcgt
     7081 gttttgggta acaaaagttg gagcaaacgc aaaagggttt cagtttaaat tatttaccac
     7141 aggcaacaag ctggctacag gccaggcagt tgaagaaggc gtggttacca ttacagcatc
     7201 tgtaatgtca aataccgagt acctggataa ttgcttttgg gtagaaagca atggtaatgt
     7261 agaagatata aaagcgttga tagacgtgca gttggcttac gtatcaaatg catcaaatgt
     7321 ttacaagtat tctgcaaaaa taccgggcag taatcttacc ggagattaca gcatattgcc
     7381 aacctacgga tcggccattg ccgctttggt tgcagatttc tctgccaagt caggcgcagg
     7441 tgttccagca acctcgttgg ccattacatc aatttcgtat gattctacgc ttgatgcgct
     7501 tactgtaact tatgatagca ccgcctatac ttctgcaacc ggcaatataa tgttggtgcc
     7561 gcctacgcca gcgcaattgg atacaggaag tgttcctgat tcagaattgc tctctgtaac
     7621 gcatccgaag gcttaacggg ttttgctatt cattataaac acaacaaagc cttcattata
     7681 aatgagggtt ttgttacaat ttgaataaaa tgacatttga aagcgtaaca tttaatgctg
     7741 catactggaa agataagaaa gagcatgagt tcataaaaca cgaaagccat catggcctta
     7801 gtgttgaaca attaaaggaa gcatttgcgg ttattaaagg caatacggca acccctgaaa
     7861 agaaaaaaga agatgccgac tttaaagggt ctttatgata aaattatcaa tatggatgtc
     7921 aataaaataa ttgacaggtc cattgataac acggtagatg ccatggcaga tattaacagg
     7981 gaaaggatgc tttcgggtgt agatgcagat ggaaaggtaa tgccctttta ttctaaacgg
     8041 tcacaggaag aatttgggaa accaaacggg ccgcatatat tgaaagatac aggagctttt
     8101 caatcaaaat tattttcccg aaggcgtgga gaaaatattg aaacaagctc caccaatgaa
     8161 aaagcggata tgcttgaaaa aagatatgga catgcagaca tttttatttt cggactaagt
     8221 ggggatttta aaaagaaata ccaggatgag tatttggctc ctgaaattaa ggtgcatata
     8281 agtgcagaaa caggtttaaa atttaaatga tgtcttattg ctggtattgc gaacagaaag
     8341 atgcttattt cactgataag ttgcaatcag taaggcaaca ggctaaaata cgggcacgtg
     8401 aaacgaaaca aactatggca attattaaag aaggaccaat cttcaaaata ttcccggcag
     8461 cagcaatccc atccggtagc atccatgttg aatttatatc aaagcatatc ggaactgcca
     8521 ctcagtaggt ttattgatgc agtagttgac aataatcttt tctctctaat taagtctggt
     8581 aatgcacagc cggatatgct gcaactctcc gcagcatgga aaaacatcct tgatgaatat
     8641 actgaggcca tgggcagcca tgaatataaa ctgtatatct cgctgctgag agaaaaggaa
     8701 cagttaataa ttgattacaa cctcatccgt tcattagttg atgcaatgca caatgtacag
     8761 gattacatta tttcagaggg ctttgtggta aatgatgatg tcatcaactt tcagaaagaa
     8821 tcaggaaaat ccctttctga agttttgcga tacaatttta ttttcaata
//
LOCUS       DAORTI010000135         8836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8637_length_8836_cov_7.222754, whole genome shotgun sequence.
ACCESSION   DAORTI010000135 DAORTI010000000
VERSION     DAORTI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8637_length_8836_cov_7.222754"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1493
                     /locus_tag="PLO70_13795"
     CDS             315..1493
                     /locus_tag="PLO70_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75589.1"
                     /translation="MPAIITHIILTLTQQKLQALLDKPFTIPVVIDNDTFPNSTPYQQ
                     INTLNQAGFTFTARELLHAIGSKTAALPANNQDECVNAAFLYSHILDFSGQNLGYKTD
                     LVMDSDLQTNRSNEIGIGIGCLLVNKQFNVNWDTLESIKGQGKRFDYRATNVGQNYAY
                     EFKGTKHRGKQNEQVQNGLEKKTEMHDRNENYDVELIISTFLGPANQQPRIILADPPF
                     QGFEDEFTEDAETIYSLRHLSRLAQFIGNPPLGRILYMQSRELIEKKNIDQIQELGER
                     LYLKEIRVIENLFETKKLIPLNFENTNYVGRWISYWQPQRKLTRKQVFEMPEIAANKR
                     LEVFQGVRRDLYELMTNNEITDLKSKLRYERTKIQTPENIEFMTFTDGSIMGYRLLDV
                     "
     gene            1704..2153
                     /locus_tag="PLO70_13800"
     CDS             1704..2153
                     /locus_tag="PLO70_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75590.1"
                     /translation="MENATLKKKHADQLIFLGVLLFLLGLVTGLVVPVFTNPRLGVSS
                     HIEGVLNGIFLIVLGLIWHKVNLSNRWLNIGFWLALYGTFTNWLGILIAAIFNAGGML
                     GIAANGKEGSPLAEATVQFCLVSLSIAMLVVAVLVLLGLKKNINQAN"
     gene            2230..2808
                     /gene="gmk"
                     /locus_tag="PLO70_13805"
     CDS             2230..2808
                     /gene="gmk"
                     /locus_tag="PLO70_13805"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQZ75591.1"
                     /translation="MAAYLNKLIIIAAPSGAGKTSVTRHLLKTFPDELVFSISCATRQ
                     PRNQEKHGVDYYFISADEFRQRIESHEFAEWEMVYHDKYYGTLKTELQRIWHQHKAPL
                     LDVDVKGGINIQSQYPEQSLSLFIEPPSIKELEQRLKKRGTETPESLKARLDKASFEL
                     SFKNRFDHIIMNDNLERACSEAEKIVRNYLNQ"
     gene            2884..4134
                     /locus_tag="PLO70_13810"
     CDS             2884..4134
                     /locus_tag="PLO70_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQZ75592.1"
                     /translation="MIQWNSIVFFILTLLLMGFFAGIEMAFFSANRLSLEIKKKQGDS
                     SMQILSGFLESPARFLGTTLLGFNIFLVFLGLQISTVMQPIWKYFRIGSDAVHILVEI
                     ALATFIVLLIAEFIPRAIFRAKSNWLLLSLARVTNVFYQLLFPIAAGLIDLAEWILKY
                     VFNIRLDKNKEALKRSDLKHLFQQRTDDDRQERNTQLMENAQELPKVKIRQCLVPRKE
                     IIGIDNKSSLEELKQKFIDTKLSKLVVFENNIDHIIGYVHQLDLFKKPAHIEDVLLPI
                     PAVPESMSATDLIGKFSKERKSIAWVVDEFGGTAGIITMEDVLEELFGEIQDEYDTEE
                     FVEKQLAENEYIFSGRLELDYLKEKYGFEFAQEDAETLSGYIINYHETIPRQKERIIV
                     DDYEFDIMHVTDTRIETVKMKRLK"
     assembly_gap    4141..4240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4241..4466
                     /locus_tag="PLO70_13815"
     CDS             <4241..4466
                     /locus_tag="PLO70_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75593.1"
                     /translation="LNLINMQTLDTLEVYIAAKTFRKEVITIATSCFKSQFCITLTTA
                     GTAIHSSNIAVLRCVRCGFCSFEIASNRYL"
     gene            4585..5439
                     /gene="tatC"
                     /locus_tag="PLO70_13820"
     CDS             4585..5439
                     /gene="tatC"
                     /locus_tag="PLO70_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQZ75594.1"
                     /translation="MALLDFFNKREKDAKGEMTFIDHLEELRWHVIRSVIAVLIGAVV
                     VFIYSDFVVDDILFAPTRSDFISARWLCSLGHKIGIGDTLCFPQVDAKFLENTMTGQF
                     IASFTLAFIGGFILAFPYIFWEFWKFIRPALSEKELKKTRGVIFWVSLLFFTGVLFGY
                     FILTPFMVNFYFNYKLSPQIQIMPSFSDYLENLIYTTAGIGVLFQMPLLVMVLARIGV
                     VTGRFLRKYRRHAFVIILIAAAIITPSTDPFSLTIVTIPLYLLFEASIIIASRINKRQ
                     EKEQKEWS"
     gene            5430..5648
                     /locus_tag="PLO70_13825"
     CDS             5430..5648
                     /locus_tag="PLO70_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75595.1"
                     /translation="MELIPALVVSQFEKSCQPCHPEFIEGCRRCQPVPIAIGIAEGDY
                     EAGFDKLNLTVRLKEEYIFQKEIQHLLS"
     gene            5723..6178
                     /gene="rpiB"
                     /locus_tag="PLO70_13830"
     CDS             5723..6178
                     /gene="rpiB"
                     /locus_tag="PLO70_13830"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQZ75596.1"
                     /translation="MHSPFDLHKPIAIGSDHAGFDYKEELISFLEGKGLHFTDFGTHS
                     KDSVDYPDFAHPVANAVEKGEAAFGILICGSANGVAITANKHQGIRAAICWGEELAQL
                     SRAHNDANIICIPARFVREGDAEKMVAVFMTTSFEGGRHANRVNKIACA"
     gene            6258..7802
                     /locus_tag="PLO70_13835"
     CDS             6258..7802
                     /locus_tag="PLO70_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQZ75597.1"
                     /translation="MIKKIIACLLLSLPLFSVAQKKDKNAAKAAATITAEDMKKHLYI
                     IASKEMGGRDTPSPGLEKAANYIEEHFKSLGLAPGNNGSYRQYYSLYKDSMQDASLVI
                     NGKKLALNTDFQAMTSNYAAGLRFSEVVFAGYGISDGDKRNDYKDLNVTGKLVLIADG
                     IPADYKPSQQGFSSPSGVTGKMNAALSRGASAIMIIYNNFPRRAGTVSSNWSMNGFKA
                     TQSLFGFTISADAAAKILGEDGKTLLEKIKANAVAAKTYKAEVELNYSKTTKTTQVSN
                     VLALLEGTDKKEEYVVITAHYDHIGTRNDTTIYYGADDDGSGTTGILELAQAFAEAKK
                     AGKGPKRSILFMTVSGEEKGLWGSRYYSEHPVYPLDKTTVDLNIDMIGRIGNEYRKDK
                     DSANYVYIIGDDKLSSDLATITDQVNSTYSKMKLDRKYNDPNDPNRFYFRSDHYNFAE
                     KGVPIIFYFNGVHPDYHRPTDTPDKINYPLMAKRAQLVFYTAWEMANRNDMLKRDLKL
                     EKPKGF"
     gene            complement(7974..8453)
                     /locus_tag="PLO70_13840"
     CDS             complement(7974..8453)
                     /locus_tag="PLO70_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75598.1"
                     /translation="MKKLLICLLTVITAFSMDAQEKVTKLNVIGNWKIVSMELPGTVY
                     YNLENDSIAPREALLTQIGSGKDSTDALATMKTSLAMLKGMSFIFNADGKGEITDGMN
                     PAKDITYSVDEAGAVITTVTLENDAKEDLKASFVKELLRMEFEDTENHIVFLFRKAK"
     gene            complement(8484..>8836)
                     /locus_tag="PLO70_13845"
     CDS             complement(8484..>8836)
                     /locus_tag="PLO70_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQZ75599.1"
                     /translation="STVKSGIEYMVWLTEWYRNNSAGKGVGFFQVGGGIAGDFPICVV
                     PMMYQDLEWHDVPFWSYFCQISDSTTSYGSYSGAVPNEKITWGKLDINTPKFIVESDA
                     TIVAPLIFAWVLGW"
BASE COUNT         2624 a         1856 c         1827 g         2429 t
ORIGIN      
        1 aaccaaagat tgcaagagag tgtttttttc tccaccgaaa aacgcaccct ttcttttttt
       61 acaaatttat tttccccacc gcacgggttc acagcgaaca cgaactgttg tgaatctaat
      121 gtaaatcgct tcggacaaaa tgtgtgcata cttgacactt caacacttcg gacagataaa
      181 agggcatccg gtaacagcgg gtttaagaaa ttgccggttc agttttataa tcaagttcag
      241 tttcccaatc aaactttcgt gctggttcac agttttgtgc tccgaaatcg ccaacttctt
      301 aaacccacca aacgttgcct gcaattataa cccacatcat actgacattg acacaacaaa
      361 aacttcaagc attattagat aaaccattta caatacctgt tgtcattgat aatgacactt
      421 ttcctaacag tacaccttat cagcaaatca atacactgaa tcaagcaggt tttacgttca
      481 ctgcaagaga attacttcac gccataggtt caaaaacagc agctttacca gcaaataatc
      541 aagacgaatg tgttaatgca gcattcttat attcacatat tttagacttt agcgggcaaa
      601 atttaggcta taaaactgat ttggtaatgg actctgattt acagacgaac aggagtaacg
      661 aaattggaat agggataggt tgcttgttgg taaataaaca attcaatgtc aattgggaca
      721 cattagaatc aataaaagga caaggcaaaa ggtttgatta cagagctaca aatgttggac
      781 aaaattatgc ttacgaattt aaaggaacta aacatcgagg gaaacaaaat gagcaagttc
      841 aaaatggact tgagaaaaaa acagaaatgc acgaccgtaa tgaaaattat gatgttgaac
      901 ttatcatttc gaccttttta ggaccagcaa accaacaacc gagaataata ttagcagacc
      961 caccatttca aggttttgaa gatgaattta cagaggacgc agaaacaatt tattccttaa
     1021 gacatttatc tcggttagct caatttattg gtaatccacc tttaggcaga attctttata
     1081 tgcaatcaag agaactcatt gagaaaaaaa acatagacca aattcaggaa ttaggagaac
     1141 gactatactt aaaagaaata agagtaattg agaatctctt tgaaactaaa aaattaattc
     1201 ccttaaattt tgagaataca aattatgttg gcaggtggat ttcttattgg cagcctcaaa
     1261 gaaagctaac aagaaagcaa gtttttgaaa tgcctgaaat agcagctaat aaaagacttg
     1321 aagtatttca aggagtgagg agagacttgt acgagctaat gactaataat gaaataacag
     1381 atttgaaatc aaaattgcga tatgaacgta ctaaaataca gacaccagaa aatattgaat
     1441 ttatgacttt tactgatggc tcaattatgg gctacagact actcgacgta taactgcagg
     1501 caacatgatc tgtgaaaaaa accaaacaaa tgttgctctt ttttatcgct aacaactgta
     1561 acccggggtg tacgtataaa tctcaaccgt aaatcttatc ttcaatttca gtttttcaac
     1621 cggcttctgc acctgttaaa gaaacacaga ataccagcac agaaggtagc ctttaatatt
     1681 gactacaacc agcaagtcaa acaatggaaa atgcaacgct aaagaaaaaa catgctgatc
     1741 aactgatatt cctgggcgta ctgttgtttt tactcgggct ggtaacaggc ctggtggttc
     1801 ctgtatttac caatccgcgg ttgggtgtgt ccagccatat agaaggtgta ctgaacggca
     1861 tcttccttat cgtgctggga ctgatctggc ataaggtgaa cctgtcgaac aggtggctga
     1921 atattggttt ttggctggcc ctgtatggca catttaccaa ctggctgggc atcctgatcg
     1981 ctgccatctt caatgccggc ggcatgctgg gtatagccgc caatggcaaa gagggatcgc
     2041 cactggcaga ggccacggtg caattttgcc ttgtgtcctt atccattgcc atgctggtgg
     2101 ttgctgtgct agtgctgctg ggccttaaga aaaatataaa ccaggccaac taatacggag
     2161 caccggtgca gtatattttt tccttttacc tgcctgatta ataattacat ttgtccactt
     2221 aaaggagaca tggctgctta cctcaataaa ctcatcatca ttgccgcacc atccggtgcc
     2281 ggtaaaacat cggtgaccag gcatttgctg aaaaccttcc cggatgaact ggttttttcc
     2341 atttcctgcg ccacccggca gccccgcaac caggaaaagc acggcgtgga ttattacttc
     2401 atctccgctg atgaattcag gcaacgtatc gaaagccatg aatttgcaga atgggaaatg
     2461 gtgtaccatg ataaatacta tggcaccctg aaaactgaac tgcaacgcat ctggcaccag
     2521 cacaaagccc cgctgctgga tgtggatgta aaaggtggta tcaacatcca gtcgcaatac
     2581 ccggagcaat cattgtcctt atttatagaa ccaccctcta ttaaggaact ggagcagcgg
     2641 ctgaagaaaa ggggtaccga aaccccggaa tcgctcaaag cccggctgga caaagcctct
     2701 tttgaacttt cttttaaaaa ccggttcgac catatcatta tgaacgacaa cctggaaaga
     2761 gcctgcagtg aagcggaaaa gatcgtacgt aattacctga accaatagga gtttattacc
     2821 tgcccggggg caatcccggc tcctcactca ctattatccg ctcccctttt ttatctttac
     2881 cgcatgattc aatggaattc tatcgtattt tttatcctta cccttttgct gatggggttt
     2941 ttcgccggca ttgaaatggc ctttttcagt gccaaccggc tgagcctgga gataaaaaag
     3001 aaacaaggcg acagttccat gcagatcttg tccggctttt tggaatcccc ggcccgtttc
     3061 ctgggtacca cgctgttggg tttcaatatt tttcttgttt tcctgggcct gcagataagt
     3121 accgtaatgc agcccatctg gaagtatttc cgcatcggct cagatgctgt acacatcctg
     3181 gtagaaattg cactggccac ttttattgta ttgctgatag ccgaatttat tccccgggcc
     3241 attttcaggg ccaaaagcaa ttggctgctg cttagcctgg caagggtgac caatgttttt
     3301 taccagttgc tgttcccgat agctgccgga ctgatagacc tggcagaatg gatcttaaaa
     3361 tatgttttca atatccggct ggataaaaac aaagaagcgc tgaaacgcag cgacctgaaa
     3421 catctttttc agcaacgaac cgatgatgac aggcaggaac gcaacaccca gctgatggag
     3481 aatgcccagg agctgccgaa agtgaagatc aggcaatgcc tggtaccacg aaaagagatc
     3541 attggtattg ataacaaatc ctcactggag gaactaaagc aaaaatttat agataccaaa
     3601 ctcagtaaac tggtggtatt tgaaaataat attgatcata tcatcggtta tgtgcaccag
     3661 cttgacctgt ttaaaaagcc ggctcatatt gaagatgtgc tgctgccaat tcctgctgta
     3721 ccggaaagta tgagtgccac cgatcttatc ggcaaatttt ccaaagaaag aaaaagcatt
     3781 gcctgggtag tggatgaatt tggcggcacc gccggcatta ttacgatgga agatgtgctg
     3841 gaagaattgt ttggtgagat acaggatgaa tatgacacgg aagagtttgt agaaaaacag
     3901 ctggcagaga atgaatatat tttttccggc cgtcttgaac tggactacct gaaagaaaaa
     3961 tatggttttg agtttgcgca ggaagacgca gagacccttt ccgggtatat catcaattac
     4021 cacgaaacca tcccccgcca gaaagaacgc attatcgtag atgactatga atttgatatc
     4081 atgcatgtaa cggatacccg tattgaaacg gtgaagatga agaggttgaa gtgaagtggg
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn actaaacctt atcaatatgc
     4261 aaacactgga cacccttgag gtttatattg ctgcaaaaac atttcgaaag gaggtcatta
     4321 ctatagctac aagctgtttc aaaagtcaat tttgtattac attaaccact gcgggcacgg
     4381 cgatccacag cagcaatatc gctgtgttac gttgtgttcg ctgtggtttt tgcagttttg
     4441 agatagcttc taaccgatat ttatagaaat gaaaaataag caggaaggat gatgcggatt
     4501 aagtacccgg ttaaccaatc caccaattaa cccaatcaac ctaatccacc aatctcctac
     4561 ctttgcgaca ctttactaaa accaatggcc ctactcgatt tttttaataa gcgggaaaaa
     4621 gatgcaaaag gtgaaatgac ctttatcgac cacctggagg aattgcgctg gcatgttatc
     4681 cgttcggtga tcgctgtgct tattggcgct gttgttgttt ttatctattc cgattttgtt
     4741 gttgatgata tcctgtttgc acctacccgt tctgatttta tttctgcccg ctggctctgc
     4801 agtctcgggc ataaaatagg cattggagat acactgtgtt ttccacaggt ggacgccaaa
     4861 ttcctggaaa ataccatgac gggccagttt attgcttcct ttacactggc atttatcggt
     4921 ggctttatcc tggctttccc gtacatattc tgggagttct ggaaatttat acgaccggct
     4981 ttatcagaaa aagaactgaa gaaaacaaga ggtgtaatat tctgggtatc cctgttattt
     5041 tttaccgggg tgttgtttgg ttatttcatc cttaccccgt tcatggttaa tttttatttt
     5101 aattataaac tcagcccgca gatacagatc atgccttcct tctccgatta cctggagaat
     5161 cttatttata ctactgcagg tatcggggta ttattccaga tgccattact ggtgatggta
     5221 ctggcaagga ttggcgtggt aacgggccgc ttcctcagaa aataccgtcg tcatgctttt
     5281 gttattatat tgatagccgc ggccatcatc actccttcta ccgatccttt cagtttgacc
     5341 attgtaacaa taccgctcta ccttttattc gaagccagca tcatcattgc ttcccgcatc
     5401 aataaaagac aggaaaaaga gcaaaaagaa tggagctgat ccctgctttg gtagtgtctc
     5461 agttcgaaaa gtcttgtcag ccttgtcatc ctgagtttat cgaaggatgc cgaagatgtc
     5521 agcctgtccc gatagctatc gggattgccg aaggcgatta tgaagccggt ttcgacaagc
     5581 tcaacctgac agttcgttta aaagaagaat acatttttca aaaggagata caacacctgc
     5641 tttcctgatt atagttggat atgtatagtt cagtaccttt gtacaagttt attggtagta
     5701 cgattcactt aaaacgttcc tcatgcattc tccttttgat cttcataagc cgattgccat
     5761 cggcagtgac cacgcaggat ttgactacaa agaagaactg atctctttcc tggaaggaaa
     5821 aggattacac tttacagatt ttggtacgca tagcaaagac tccgttgatt atcccgactt
     5881 tgcccatcct gttgccaatg cggtagaaaa aggtgaagca gcatttggta tcctgatctg
     5941 cggcagtgct aatggtgtgg ctattactgc caataaacac cagggcatcc gtgctgccat
     6001 ttgctggggc gaagaactgg ctcaactgtc ccgtgcacat aacgatgcca atattatctg
     6061 tatccctgca agatttgtgc gggaaggtga tgcagaaaaa atggtagctg tatttatgac
     6121 cacctcattt gaaggaggcc ggcatgccaa ccgggtgaat aagatcgcct gcgcataatt
     6181 ccattaccaa tcatccctat tttatccctt ctttttccgg aagggattat ttttaaccct
     6241 catacatcaa tacaactatg atcaaaaaaa ttattgcctg ccttttactt tcgttgcccc
     6301 ttttttcagt ggcacaaaaa aaagataaga acgctgcaaa agctgctgcc accattacag
     6361 cggaggacat gaaaaaacat ttgtacatca ttgccagtaa agaaatggga ggaagggata
     6421 ctccctcccc gggattggag aaagcggcaa actatatcga agaacatttt aagtcactgg
     6481 gcctggcgcc gggtaataac ggaagctatc gccagtatta ctctttgtat aaagattcca
     6541 tgcaggatgc ttcgttggta atcaatggga aaaaattggc tttgaatacc gatttccagg
     6601 ccatgaccag taattatgct gcaggcctcc gtttttccga agtggttttt gcagggtatg
     6661 gcatcagtga tggtgacaaa agaaatgact ataaagacct gaatgtaacc ggtaaattag
     6721 tgctgatcgc agatggtata ccggctgatt acaaaccatc acaacaggga ttttcttctc
     6781 cctccggtgt aacaggtaaa atgaatgctg ccctgagcag gggtgccagt gcaataatga
     6841 ttatttataa taattttccg agaagggctg gtactgtgag cagtaactgg agtatgaatg
     6901 ggtttaaagc cacacaatca ctttttggct ttactatctc tgcggatgct gccgctaaaa
     6961 tattgggcga agacgggaaa acattgctgg aaaaaataaa agcgaatgcg gtagcagcaa
     7021 aaacatacaa agctgaagtg gaactgaatt attccaagac aactaaaaca acacaggttt
     7081 cgaatgtgct tgcgctattg gagggaactg ataagaaaga ggagtatgta gtgatcactg
     7141 cccactatga tcatattggt acaagaaatg ataccaccat ttattatgga gcagatgatg
     7201 atggcagtgg caccaccggt atattagaac tggcccaggc ttttgcagaa gccaaaaaag
     7261 ctggtaaggg tccaaagaga agtatactgt ttatgacagt aagcggtgag gaaaaaggac
     7321 tatggggcag caggtattat tcagagcacc cggtttatcc ccttgacaaa acaacagtgg
     7381 acctgaatat agatatgatc ggccgtattg gaaatgaata caggaaggat aaagactccg
     7441 ccaactatgt atacatcatt ggtgatgata agttaagctc tgacctggcc accataaccg
     7501 accaggtaaa cagcacctac agcaaaatga agttagacag gaagtataat gaccccaatg
     7561 atccaaatcg tttttacttt cgcagcgacc attacaattt tgcggagaaa ggtgtgccga
     7621 ttatttttta tttcaatggt gtgcacccgg attatcaccg gccaacagat actcctgata
     7681 aaatcaatta cccgctgatg gccaaaaggg cacaactcgt tttttacacg gcctgggaaa
     7741 tggctaacag gaacgatatg ctgaaacggg acctgaagct ggaaaagcct aaaggatttt
     7801 aattttattt ctattttgaa caagtaaaac cttccggtat ggaaggtttt ttttattttt
     7861 ttaaacttca ggcaactttc tttgtctatt atatgtctgt agcaaagaat cacttcaatt
     7921 tttgatccct acctttctac ataaaaatga tccccggcat accggggatt attttattta
     7981 gccttcctga aaagaaatac aatatgattt tccgtgtctt caaactccat ccggagcaac
     8041 tcttttacaa aagaggcttt taaatcttct ttggcatcat tttccagcgt aacggtggtg
     8101 ataacagcac ctgcctcatc tacactataa gtaatgtctt tggccgggtt cattccatcc
     8161 gtaatttctc ctttaccatc cgcattaaaa ataaatgaca tccccttcag cattgcgagg
     8221 gaggttttca ttgttgccag cgcatcagta ctgtcctttc ctgatcctat ctgtgtcagc
     8281 aaggcttccc tcggcgcaat agaatcgttt tcgaggttgt aatacacggt accgggtaat
     8341 tccattgaaa cgatcttcca atttccaatc acatttaatt ttgtcacctt ttcctgtgca
     8401 tccatactga aagctgtaat aactgtcagt aaacaaatga gtaatttctt cattaatagt
     8461 agtttgtata aaaatagaat tgtttaccaa cctaaaaccc aggcaaatat cagcggcgca
     8521 actatggttg cgtcactttc tacaatgaac ttgggagtgt ttatatccag tttgccccag
     8581 gttattttct cattcggcac cgcgccggaa taagagccat aagaagtggt tgagtcactg
     8641 atctggcaga aataactcca gaaaggaaca tcatgccact ccaggtcctg gtacatcatc
     8701 ggcaccacac aaatcgggaa atcaccggca atgccgccgc ctacctggaa aaagccgacc
     8761 cctttaccag ctgaattatt gcgataccat tccgtaagcc ataccatgta ttcaataccg
     8821 cttttaactg tacttg
//
LOCUS       DAORTI010000136         8764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8750_length_8764_cov_6.269032, whole genome shotgun sequence.
ACCESSION   DAORTI010000136 DAORTI010000000
VERSION     DAORTI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_8764_cov_6.269032"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..470)
                     /locus_tag="PLO70_13850"
     CDS             complement(195..470)
                     /locus_tag="PLO70_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQZ75600.1"
                     /translation="MLYTISITVSGKVQGVFFRQNTKEKAIALGITGSVQNLHDGNVN
                     IIATGTREQLDELTAWCRQGPRRAIVKNIITKEIPVQSFNDFSIDRS"
     gene            complement(475..948)
                     /locus_tag="PLO70_13855"
     CDS             complement(475..948)
                     /locus_tag="PLO70_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQZ75601.1"
                     /translation="MAEDLFITKGAKAEQYQSLIPQIKGLLEGETDLVANMANVVAAL
                     KEQFGWLWVGFYVVKDDELVLAPFQGPVACTRIKKGRGVCGTSWAQAQTLIVPDVEKF
                     PGHIACSSLSRSEIVLPVIRNNEVVAVLDADSAELNQFDTVDQQYLEEILAMVNF"
     gene            1071..3875
                     /locus_tag="PLO70_13860"
     CDS             1071..3875
                     /locus_tag="PLO70_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75602.1"
                     /translation="MRKLFFLFFSLLAYSFLFSQQFGGNPPSTKWQQINTDSARIIFP
                     AGMDSQANRVAAIVHYMAQHKPVSLGDQLKKINIVLQNQTVIPNGYVQLGPYRSEFFL
                     TPEMNNFGQGSVSWTDQLALHEYRHVQQFNNFNNGLSKVMKTLFGEEGFALAINASIP
                     DWFYEGDAVFNETILSKQGRGRIPLFLNSYPSIWQAGKKYSWMKLRNGSLKDYVPNHY
                     NLGYLLVNYGREKFGTDFWAKVTKDASAFKGLFYPFQVAIKKHAGVDYKTFRKDAFAH
                     YQKDVQRVSVSRNDFLLPVNSNYVTSYYFPYSAGDDSLLYLKASYRHRPAFYIKDKNG
                     EHKLKMRDISIDEQFSYRNGKIVYAAYENNPRWGWLDYSVIKLLDIQTGQQKTLTKRT
                     RYFTPDVSADGSKVVAVQVGTNGKSELHILNVQDGEILQRIHSTEISLFTDPKFIDDN
                     SIVSAVRLKDGRMTLAAAEIATGATIRLTPPSFNVVGYPSVSKDMIYFTATYEGNDDV
                     FVLRMSDKKIFKISSGPLGNYYVNAGNGKITWSAFTAEGYQLQQVAEKDIVWNEIGTA
                     TAERLTEKFAVSNAGEAGDILLGIIPERNFAVSKYNKGTRLLNFHSWRPYYEDPIFTF
                     SLYGENVLNTLQTELYYLYNENEKTSAVGFGTVYGAMFPYLNIGSELTFNREDVIGNR
                     LRVWNQLDSRIGLSVPLSYTKGQAFRNFTISSFYVLRNEFNKGFYKDSLGNTSFSYLS
                     HAISWSQQVQRAVQHIYPRFAYSVSSSYRHAITKYEGSQFNGNVSLYIPGLLSTHNLL
                     LTGSWQERDTLSQVSFANRFSYSRGYSGRYFSRMWRLSANYHFPLIYPDWGFGNILYL
                     QRIRANAFYDFTKVYSRDKSQTRDQRSVGGEVFIDTKWWNQYPLTFGFRISRLLDQDQ
                     FDGFKGTVFEVVLPVSIIPR"
     gene            complement(3951..6566)
                     /locus_tag="PLO70_13865"
     CDS             complement(3951..6566)
                     /locus_tag="PLO70_13865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQZ75603.1"
                     /translation="MELSKNFDHSAAEQKWYQHWLDKKYFHSEPDDREPYTIVIPPPN
                     VTGVLHMGHCLNNTIQDILIRRARMQGKNACWVPGTDHASIATEAKVVQMLRERGIAK
                     SSLSREDFLKYAWEWKEKYGGIILQQLKKMGCSLDWERTSFTMDPDYYQSVISVFIDL
                     YNKGYIYRGKRMINWDVKAKTALSDEEVIRKETQQKLYHLRYKLDMPGDEYIVIATVR
                     PETIMGDTAICVHPDDERYKHLHGKFAFVPLIDRRIPIITDDYVTMEFGTGALKVTPA
                     HDSNDNQLGQKHNLDVVDIFNEDGTLNTEAQIYIGEDRFEARKKIAKELEEKGFLLKT
                     EDYTSEVGYSERTDAVVEPRLSLQWWVDMKKISGPALSAVMEDDVKFYPSKFKNLYRH
                     WMENIKDWCISRQLWWGHRIPAWYDNEGRFVVAATEQEAKELFSSQHGVSNAFLKQDE
                     DCLDTWFSSWLWPFEVFKGYSNPNNPDVNYYYPTNTLVTAPEIIFFWVARMIMAGYEY
                     KGQKPFNEVYFTGIVRDKQGRKMSKSLGNSPDLLEMIDKDGADAVRFGIMISSPAGND
                     LMWDPSGNDQGRFFINKMWNALKLVKMWSLRQAQDESLAANNFAVEWFENRLNEVRIQ
                     LDEQFRDFRLSESLKTLYSLIWDDFCSWYLEWVKPGFEQPIDKGVYEKTIGFFEELMQ
                     LLHPFLPFVTEEIYHQLKEQNDDLTVKQYPAVRTTGKTILSDAILLKEVITAIRDARN
                     KNQLKPKDSIRLHIQTDNTDTYKAIESILLKQVNAESISYTSDSVVNSITVVVQKDKF
                     FIETTTLLDTASQKQQLQKDLDYLKGFLISVEKKLGNERFVQNAKPEVIEIERKKKAD
                     AEEKIKVIENSLAAL"
     gene            complement(6692..7117)
                     /locus_tag="PLO70_13870"
     CDS             complement(6692..7117)
                     /locus_tag="PLO70_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75604.1"
                     /translation="MKNDYIRIMLVDDHQLVRESFKLLLENNPRFQVIADCDNGNAAL
                     EQFQSLNPDIMLVDINMSPVNGFELTRRIIETMPMAKIIGLSVHNQAKYATRMMELGA
                     RGFLTKTSPLEEINRGILAVHRGEHYICEEVRLNMPPSE"
     gene            7535..8266
                     /locus_tag="PLO70_13875"
     CDS             7535..8266
                     /locus_tag="PLO70_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQZ75605.1"
                     /translation="MKKELNSWYSPSLNKEMPIATYGDYGFALLLVPTAAADYLEYER
                     FQLMHHIAPFINQGKVKVFSIDSINNESWLNNHMDPRHKSVRHQQWNDYVFNEVVPFI
                     RNSTSPDTPIITCGASFGALHSMNLFLKRPDIINGVIAMSGVYDLTEYTKGHYDEDVY
                     FNSPMHFMPNLGDHSVLEQIRRSKNIHIVTGSGSYEDPTSSGKFAKILYDKGIWYELD
                     VWGEEWPHDWDTWRAMLPHYLGSKF"
     gene            complement(8245..>8764)
                     /gene="aat"
                     /locus_tag="PLO70_13880"
     CDS             complement(8245..>8764)
                     /gene="aat"
                     /locus_tag="PLO70_13880"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQZ75606.1"
                     /translation="PWYEGEHILWWSPDPRFVLFPGELKVSKSIKPLLKRNEFDFTIN
                     KAFQQVILRCKETKRPGQEGTWITNEVERAYCKMHESGYAHSAEVWKNGELVGGLYGI
                     KMGKVFFGESMFSKQSNASRFAFTRYVQLLKEEGILLIDCQVYTEYLESFGARMIPRK
                     EFIQLLQNLLPR"
BASE COUNT         2465 a         1776 c         1778 g         2745 t
ORIGIN      
        1 agaaatgact ggggctggaa ggaagaatat gacctggcag ctatgacgaa agatatgttt
       61 gataacctga aataagcccg gcaaaaaata cgtttttact gagaccctga ttacgacgac
      121 ataattttgt tgtcttttat tttaacagat cccgggtgta tgacaaaatt aaaattgaga
      181 tttggttgaa actattatga tctgtcaatg gaaaaatcgt tgaatgattg tacaggaatc
      241 tcttttgtta taatgttttt tacaattgcc ctccggggtc cctgccggca ccaggcggtg
      301 agctcgtcta attgttctct tgttcctgta gctattatat tcacattgcc atcatgaaga
      361 ttttgtaccg atccggttat acccagtgct attgcttttt ctttggtgtt ttgccggaag
      421 aatactccct gtactttgcc acttacagtt attgagatag tgtaaagcat agatttaaaa
      481 attgaccatt gccaggatct cttcgaggta ttgctgatca actgtatcaa actggttaag
      541 ctcagcactg tctgcatcta atacagctac tacttcatta ttacggatga caggcagcac
      601 aatctcagat cttgataaac tgctgcaggc aatatgtccg ggaaattttt caacatccgg
      661 tacaatcaac gtttgtgcct gcgcccagct ggtaccacat actcctcttc ctttttttat
      721 tcttgtacat gctaccggtc cctggaaggg ggccagtacc aactcatcat cctttaccac
      781 ataaaatccg acccacagcc agccgaactg ttctttcaag gctgcaacaa cattggccat
      841 attggctacg agatccgtct caccttcgag tagtcccttt atttgaggaa taagcgactg
      901 gtattgttca gcttttgcac cttttgttat aaataaatct tctgccattt tgcaaagata
      961 aggctgcacg atgagccaat gttgtgtaca tcaatcaagt gacagttcat agcacgggta
     1021 ctttataatc actttttcct gcgtaacttc cgctataaat atccactgcc atgcgaaagc
     1081 ttttttttct atttttttca ttattggctt acagtttcct tttctcccaa cagtttggag
     1141 gaaatcctcc ttccacaaaa tggcaacaga tcaatacaga ttctgcccgg atcatttttc
     1201 ctgcaggtat ggatagccag gccaaccggg tagctgcaat cgttcattat atggcgcagc
     1261 ataaacctgt gtcccttggt gatcaattga agaagataaa tattgtattg caaaaccaga
     1321 cggtcattcc taatgggtat gtacaacttg gtccgtatcg cagtgaattc tttctcacac
     1381 cagaaatgaa caattttgga cagggtagtg taagctggac tgaccagtta gcattgcatg
     1441 agtacaggca tgttcagcag ttcaataatt tcaataatgg gctaagtaag gtcatgaaaa
     1501 ccttatttgg tgaagaagga tttgccctgg ctattaatgc ctccatccct gattggtttt
     1561 atgaaggtga cgccgtcttt aacgagacaa tactcagtaa gcagggcagg ggcaggatac
     1621 cactttttct gaatagttac ccatcaatat ggcaagccgg aaagaagtat tcctggatga
     1681 aattaaggaa tggctcgttg aaggattatg tacctaatca ttataacctt ggttatttac
     1741 tggtgaacta tggcagggaa aaatttggta cagacttttg ggcaaaagtg accaaagatg
     1801 ccagtgcttt taaaggatta ttttatcctt tccaggtagc cattaagaaa catgccgggg
     1861 tagattataa aactttccgg aaggatgcat ttgctcatta tcaaaaagat gtacagcggg
     1921 tatcagtgtc cagaaatgat tttctgctgc ctgtgaatag caattacgtt actagttatt
     1981 atttccctta tagtgcaggt gatgactcat tgctctattt aaaagccagt tatcgtcacc
     2041 gacccgcatt ttatataaaa gataaaaatg gggaacataa gctgaaaatg agggatatct
     2101 ctatcgatga acaattttcc tatcgcaatg gtaagatcgt ttatgcagct tatgaaaata
     2161 atccccgttg gggctggctg gattacagtg tgataaaact gctggatata caaacaggac
     2221 agcaaaagac actaacaaaa agaacgagat attttactcc cgatgtttca gcagatggct
     2281 caaaagtagt ggctgtgcag gtgggtacaa atggcaaaag cgaattacat atcttaaatg
     2341 tacaagatgg ggaaatcctg caacggatcc attcaacaga aataagtttg tttactgacc
     2401 ccaaattcat agatgataat tcaattgtca gtgctgtgag attaaaagat ggcagaatga
     2461 cactggctgc cgctgagatt gctactggtg ctacaataag gcttacacct ccttctttta
     2521 acgtagttgg atatcccagt gtaagcaagg acatgattta tttcacagcc acttatgaag
     2581 ggaatgatga tgtatttgtt ttgcggatga gtgataagaa gatatttaag atcagcagcg
     2641 gcccattggg taactattat gtgaatgccg gcaacggaaa aattacctgg tctgcattta
     2701 cggccgaagg ctaccaatta caacaagtgg ctgaaaagga tattgtatgg aatgagattg
     2761 gtactgcaac tgctgaacgt ctgacagaaa aatttgcagt aagtaatgct ggtgaagctg
     2821 gagatatatt actgggtatt attccggaaa ggaattttgc tgtgagtaaa tataataaag
     2881 gaacaaggct gctgaacttt catagctgga ggccttatta tgaagacccg atctttactt
     2941 tttccctcta tggagaaaat gtgctcaata ctttgcagac agaactctat tacctgtaca
     3001 atgaaaatga aaagaccagt gctgttggct ttggaacagt atatggtgct atgtttccat
     3061 acctcaatat tggttcagag cttactttta acagggagga tgtgatcggt aaccgtctgc
     3121 gggtttggaa tcaattggac agccgcatcg ggttaagtgt gccattaagc tacactaagg
     3181 gccaggcctt caggaatttt actatcagca gcttttatgt attgcggaat gagttcaata
     3241 aaggatttta caaggattca ttagggaata catcatttag ttatttgtca catgccatat
     3301 cctggagcca gcaggtacaa agagcggtac agcatattta tccacggttt gcttattctg
     3361 tatcctcttc ctatcgccat gcgattacaa aatacgaagg ctcgcaattc aatggcaacg
     3421 tttcgttgta tattccgggc ttgctttcta cgcacaattt attgctgaca ggttcctggc
     3481 aggagagaga tacgttgtcg caggtttctt ttgccaatcg cttttcttat tcgaggggtt
     3541 attccggaag atatttttcc cggatgtgga ggttgtctgc gaactatcat tttccattga
     3601 tctatccgga ttggggattt ggtaatattt tgtacctgca acggataagg gccaatgctt
     3661 tttatgattt caccaaagtg tattcaaggg ataaatcaca aacaagggat cagcgcagtg
     3721 tgggtggtga agtatttatt gataccaaat ggtggaacca gtacccactg acttttggtt
     3781 tcaggatcag ccgtctgctt gaccaggacc agtttgatgg atttaaagga actgtatttg
     3841 aagttgtatt acctgtaagt ataattccca ggtaattaaa tgccggcata ttccggttac
     3901 acagcgttat acctgagtaa ccgtttttct ttttatggca gcgttaaaaa ttataaagcg
     3961 gccaggctat tttctattac ttttattttt tcttctgcat cagctttctt tttcctttct
     4021 atttcaatca cctctggttt tgcattttga acaaatcttt cgttgcccag tttcttttca
     4081 acagatataa ggaaaccttt caggtaatca aggtcttttt gtaattgttg tttttgcgaa
     4141 gcagtatcca gtaaagtggt tgtttcaata aagaatttat ctttctggac tactactgtt
     4201 atactgttaa caacagaatc agatgtgtaa gagatagatt cagcgtttac ctgcttgagt
     4261 agaatgcttt caatcgcttt atatgtatcc gtattgtccg tctggatatg aagacggatg
     4321 ctgtcttttg gttttagctg gtttttattt cttgcgtccc ttatagcagt aataacttct
     4381 ttcagcaata tggcatccga aagtattgtt ttaccggtag ttctcacagc cgggtattgc
     4441 ttcacagtta agtcatcgtt ttgttctttc aattgatgat aaatctcctc tgttacaaaa
     4501 ggaaggaaag gatgaagcaa ttgcatcagc tcttcaaaga aacctattgt tttctcataa
     4561 actcctttat caattggctg ttcaaaaccc ggttttaccc attctaaata ccaactgcag
     4621 aaatcatccc agataagtga gtacaatgtt ttcaatgatt cactcagccg gaaatcccta
     4681 aactgctcgt ctaactgaat tctcacttca ttcaaccggt tctcaaacca ttctaccgca
     4741 aaattgttag cggccaggct ttcatcctgg gcttgccgga gggaccacat cttcaccagc
     4801 tttaatgcat tccacatctt gttaatgaag aagcgaccct ggtcattacc ggaaggatcc
     4861 cacatcaggt cattgccggc aggcgaagag atcatgatgc caaaccggac agcatcagcg
     4921 ccatctttat cgatcatttc aagcaggtcg ggagaattcc ccaggctctt actcattttt
     4981 cttccctgtt tatcacgaac gattccagtg aagtatactt cgttgaaagg tttttgccct
     5041 ttgtattcgt aaccggccat aatcatacgg gcaacccaga agaagataat ttccggtgct
     5101 gttactaatg tattggtagg atagtaataa ttcacatccg ggttattggg attactataa
     5161 cccttaaaca cttcaaaagg ccaaagccag gaagaaaacc aggtgtcaag gcaatcttca
     5221 tcctgtttta aaaaagcatt acttactccg tgttgcgaac tgaataattc ttttgcttcc
     5281 tgttctgttg cagccactac aaaccttcct tcgttatcat accatgccgg gatcctatga
     5341 ccccaccaca gttgtctcga aatacaccaa tctttgatat tctccatcca gtgacggtaa
     5401 aggttcttga atttggaagg gtagaatttt acatcatctt ccataacggc agacaatgca
     5461 ggtcctgata tttttttcat atctacccac cattgcagag ataaccttgg ttcaaccact
     5521 gcatccgttc tttcactgta gcccacttcg ctggtgtagt cttctgtttt tagtaagaaa
     5581 cctttttctt ccagttcttt agctattttt tttcttgctt cgaaccggtc ttcaccaata
     5641 taaatctgcg cttcggtatt gagtgttccg tcttcattaa atatatcaac tacatccagg
     5701 ttatgttttt gccccagttg attgtcattg ctatcatggg ccggagttac tttcaatgca
     5761 ccggttccaa attccatggt tacatagtca tctgtaatga tggggatacg acggtcaatt
     5821 aacggaacga aggcgaattt gccatgcagg tgtttatatc gttcatcatc cgggtgaacg
     5881 caaatagctg tatcacccat tattgtttca gggcgaaccg tagcaattac aatatattca
     5941 tcaccgggca tatccagctt ataccgcagg tggtaaagtt tttgctgcgt ctcttttctt
     6001 attacttctt catcacttaa tgctgttttt gcttttacat cccagtttat cattcgtttg
     6061 ccccggtaga tataaccttt gttgtaaaga tcaataaata cactaatgac cgattggtaa
     6121 taatcgggat ccattgtaaa actggtacgt tcccaatcaa gactgcaacc cattttcttc
     6181 aactgttgca gaatgatgcc gccatatttt tctttccact cccaggcata ttttaaaaaa
     6241 tcttcccggc tcagtgatga tttggcaatt cctctttcac gaagcatctg tactactttt
     6301 gcttcggtag caatagaagc atggtctgtt cccggaaccc agcaggcatt ttttccctgc
     6361 atccgggctc ttctaattaa aatatcctga atagtgttgt tgaggcaatg ccccatatgc
     6421 agtacaccgg ttacattggg aggagggatg actattgtat aaggctcccg gtcgtccggt
     6481 tcgctgtgaa aatatttttt atccaaccaa tgctgatacc atttctgttc ggcggcactg
     6541 tggtcaaaat tcttgctcaa ttccatacta cttcgcttac agttttaaat gaagtaacct
     6601 ctgtaatggt tacacagtac atgtaaggcc gcaaatttac cgggttttag gtagaaattg
     6661 aaacagcagg agatatgatg agagggatgt tctattcaga aggaggcatg tttagtctta
     6721 cttcctcaca gatatagtgt tctcctctgt gaacagcaag gatacctcta ttaatttctt
     6781 ctaaaggaga cgttttggtc aggaatcccc tggcccctaa ttccatcatc cgggtagcat
     6841 acttcgcctg gttatgaaca gagagtccaa tgatctttgc catcggcatt gtttcaatta
     6901 tgcgcctggt aagttcaaac ccattaacag gagacatatt tatatctacc agcataatgt
     6961 ccgggttgag agattgaaac tgctctagtg ctgcatttcc attatcacaa tctgctataa
     7021 cctgaaaccg ggggttattt tccaataaca atttaaagga ttcacggacc aattgatgat
     7081 catcaaccag catgatacgg atatagtcat tcttcataat caaaccagtt aagttgctac
     7141 ataatcaata aacatggagg ccatgctttg ggaaaaggtt ttacacagaa acggcttgcc
     7201 aggccataat tggtagatca gtaaggattt ggatgataat gccaggttga tagccttcaa
     7261 aaagccaacc cgcttgaact aattggctgg attgaaagca aaattcaaaa ttgtcctaaa
     7321 acagtaaaaa tactgaaaac taaaagtaat attactttgt tattatacca agtaatatta
     7381 ccaaaatata tttcgtaaat ctacgataag gtacatgact gaataatatg ggcaggagtt
     7441 gcagttaaaa gcaaattttg aaatgaatgt caaaaaagag tttgactttt gccgttcctt
     7501 atttccatca taaaataaaa tgtaacctat tgcaatgaaa aaagaattga attcctggta
     7561 tagtccttct ttaaataaag aaatgccgat agccacctat ggcgattatg gatttgcatt
     7621 attgctggtg cctactgcag ctgccgatta cctcgagtat gaacgttttc agctgatgca
     7681 tcatatcgct ccatttatta accagggaaa agtaaaagtg ttttcaattg acagtatcaa
     7741 taacgaaagc tggctgaata atcatatgga tccccggcat aagtctgttc gtcatcagca
     7801 gtggaatgat tatgtgttta acgaagtggt gccatttatc cggaacagca ccagcccgga
     7861 tacgccgatc attacctgtg gcgcttcctt tggtgcctta catagtatga atttatttct
     7921 aaagcgacca gatattataa atggtgttat cgctatgagt ggagtttatg atttaactga
     7981 atacaccaaa ggccactatg atgaggatgt gtattttaac agccccatgc attttatgcc
     8041 taacctgggt gatcattcgg tactggaaca gatcaggagg agtaagaata ttcatattgt
     8101 taccggtagc gggtcgtatg aagatcctac cagcagcggt aagtttgcca aaatattata
     8161 tgataaagga atctggtatg aactggatgt atggggtgaa gagtggccgc atgactggga
     8221 tacctggagg gctatgttgc cgcactacct gggtagtaag ttctgaagca gctgaataaa
     8281 ttcttttcgc gggatcatcc gggcaccaaa actttcaagg tattctgtat agacctggca
     8341 gtctattaac aggatacctt cttcttttag cagctgaaca tacctggtaa aagcaaaccg
     8401 tgacgcatta ctttgtttgc tgaacatact ttccccgaaa aacactttgc ccattttaat
     8461 cccataaagt ccgccaacta attcaccatt cttccatacc tcggcgctgt gggcatatcc
     8521 agattcatgc attttacaat aggctctttc tacttcgttg gttatccagg ttccttcctg
     8581 cccgggtctt tttgtttctt tgcatcggag aataacttgt tggaaggctt tgttgatggt
     8641 aaaatcaaat tcgtttcttt tcagtaatgg ctttatgctt ttactcactt ttaattcgcc
     8701 gggaaataaa acaaatctcg gatcaggact ccaccagagt atatgttcac cctcgtacca
     8761 ggga
//
LOCUS       DAORTI010000137         8742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_8780_length_8742_cov_6.853805, whole genome shotgun sequence.
ACCESSION   DAORTI010000137 DAORTI010000000
VERSION     DAORTI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8780_length_8742_cov_6.853805"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="PLO70_13885"
     CDS             <1..1897
                     /locus_tag="PLO70_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQZ75607.1"
                     /translation="VSPYYRDESMILGLGFTPYHQMLQLLAKGDDIFTGGREYYSHPN
                     YRGNDKPTMIHQSSATGMQVIPTTGIAQGIQYLERYNASLLKKGINGVLPVTLCSLGD
                     ASVTEGEVSEAFQFAVLKQLPVIYLVQDNNWGISVTAQEARSMNAFEFAAGFKGMNRV
                     QVDGSDFEASYSVMKEVVDFVRRERKPYLVHAQVPLLGHHTSGVRREFYRTDEDWARH
                     QEHDPNSKLRKKLVEKGVLENELLHIEKEAAELVAGDFAKAVASPDPDPATVEDHIFV
                     ATPITEEKGERSPAGADKVIMVDAALFAIREIMEQHPEAVLYGQDVGKRLGGVFREAA
                     TLGDMFGEHRVFNTAIQEAYVVGSTVGMSAVGLRPIVEVQFADYIYPGFNQLVTEVSK
                     SCYLTCGKFPVSMILRVPIGAYGGGGPYHSGSVETTLLSIKGIKVAYPSNAADMKGLM
                     KAAYYDPNPVVMLEHKGLYWSKVPGTEDAKTTEPSKDYILPFGKANIVTQASAKAIEN
                     GESCVVITYGMGVYWAKAATKNFSQQIEVVDLRTLYPLDEALVFERVKLHGKCLVVTE
                     EQQNNSFAEALAGRISKNCFQSLDAPVDVMGALNLPAVPMNMGLEQAMLPNAEKVSAR
                     LKQLLVS"
     gene            complement(1894..3723)
                     /gene="aspS"
                     /locus_tag="PLO70_13890"
     CDS             complement(1894..3723)
                     /gene="aspS"
                     /locus_tag="PLO70_13890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQZ75608.1"
                     /translation="MYRTHTCGELRIGNNGKEVTLAGWVQTIRKFGAITFVDLRDRYG
                     ITQLLFGEELNNQLEVNPLGREFVIQATGMVNERSNKNLNIPTGEIEIVVSTFKVLNR
                     SAVPPFTIQDDTDGGDDLRMKYRFLDLRRNAVKRNLELRYAVNRAARNYLHQHNFMDI
                     ETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGYDRYYQIVKCFRD
                     EDLRADRQPEFTQIDCEMAFVEQEDILNMFEGMIKSIFKEVKGIDYTETVQRMTWEDA
                     MWNYGNDKPDIRFDMKVLNLKKPATVYTEKQDNSTLISGKDFKLFDEAETVLAIAVPG
                     CSEYSRKQTDELTEWVKRPQIGMKGLVFIKVNTDGTYKSSVDKFYSEENLKAIAEAAN
                     AKAGDLILMLAGAEERTRKAISDLRMEMANRLGLRKDTEFKLLWVLDFPLFEYDEEGN
                     RWVARHHPFTSPKPSQIETMINNNPVIENAAEYLKHPYANIKANAYDMVLNGNEIGGG
                     SIRIFQRELQEKMFAALGMDAAEQQHKFGFLLGAFEYGAPPHGGIAFGFDRLCAILGG
                     SESIRDFIAFPKNNAGRDVMLDAPATIDEKQFEELQIKLDLKN"
     gene            complement(3791..4201)
                     /locus_tag="PLO70_13895"
     CDS             complement(3791..4201)
                     /locus_tag="PLO70_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75609.1"
                     /translation="MNQPNEDQYIIPSRYRRMENMHIAFWLVKDISWCLIWKVLGIIM
                     IVPTLTVAILIAWRTRKLASELAHNLAVAFWISANSFWMISEFLGFDEKEFWGGITGK
                     QLSLIPFFIGAFILLYYYSVQRPREIKLQKTTTM"
     gene            complement(4227..5294)
                     /locus_tag="PLO70_13900"
     CDS             complement(4227..5294)
                     /locus_tag="PLO70_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ75610.1"
                     /translation="MNVDFLIVGQGISGTWLSYFLQKENKSFLVIDNNDKKAPSRLAA
                     GIINPVTGRRHVEVWMAEEILPFALEAYTRIGNELTITAISQKNIIDFFPSPQMRQSF
                     FQRIAEKGEYVADYREQDHFNKFFNYDFGCGIINPVYTAHLETIIPAWQQRLKKTNNL
                     LEEAFDINKLSVAPSKIQYDTITASAIIFCDGTGSNNNPYFKLLPFAPNKGEVLIAEI
                     PGLPDEHIYKRGMILAPLVTPGRWWVGSNYQWEFDNDKPGKEFREKTEQLLKDWLKIP
                     FTITEHLCGIRPATLERRPFVGFHPLYRNIGILNGMGTKGSSLAPFFARQLTNNILRD
                     EAIHPEADIKRFSRILSRNIS"
     gene            complement(5338..5862)
                     /locus_tag="PLO70_13905"
     CDS             complement(5338..5862)
                     /locus_tag="PLO70_13905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQZ75611.1"
                     /translation="MITLIIARHGETIENGLNICQGQTHGILSETGIRQNEKLGAQLT
                     KYQFQTIYSSPLNRAFQTAKSIQQHTPHSLLITDDRLMERQLGILQGEKYPSPYLESD
                     LYEGMETVESMAERMNNFLSHIKQKHPGETIVIVSHGYIIKVLLSLINNLPVRDFYKV
                     KLMSNSGFIEVTLE"
     gene            complement(5927..>8742)
                     /locus_tag="PLO70_13910"
     CDS             complement(5927..>8742)
                     /locus_tag="PLO70_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HQZ75612.1"
                     /translation="GSEEKWTLKISGYKNEKVAAELLASMYDASLDQFYPHGWSRPYI
                     WYNNYKMSSWNSTQNFIKSESVSRYLAMQEQKSLDKRYDYLLSNERGGVFSDQRITLR
                     GINSVSAETMTQAKEVANIQPALTGKVSGLEVRLDSIVRFDEQTQGNDVKIRKNFNET
                     AFFFPDLKTDSTGMIEFSFTMPEALTRWKFMALAHTKEAAFGSSTKEIVTQKELMVQP
                     NAPRFLREGDKMEFSSKIVNLSDKELTGTAELQLFDATTNQSVDGWFRNSFPNQYFTV
                     AAGQSEAVKFPIEVPYLFNKALVWRIVAKAGNYSDGEENAMPVLTNRMLVTETMPLPM
                     RGSGTKDFSFEKLLNSGSSESLQHYALTVEYTSNPAWYAVQALPYLMEYPYDCAEQTW
                     NRYYANSLAGYIANSSPKIKQVFEQWNIKDTAALMSNLQKNQELKAALLEETPWVLQA
                     KNETEQKKNIALLFDMVKMSEQLNSSYEKLKQMQSSNGGFVWFTGGPDDRYITQYIVT
                     GIGHLKKLKGIAAGQEAKLNAILSTAIPYLDKKLKEDYDNLIKNKTDLKKYSPGHSSI
                     QYLYMRSFFPEYEIAKASQTAYTYFRSRMQQTWVGQSKYMQGMAALALHRTGDVKTPA
                     AILKSLKETSITNEELGMYWKNQRYGWFWYEMPIETHALLIETFQEVAKDSKTVDDLK
                     TWLLKNKQTNNWKTTKATAEACYALLLQGTNWLATEPVVTINLDNMVIRSNNNKDEAG
                     TGYFKKTIEGNKVQPVMGKITVKVEETQSLPAGDWGASWGGIYWQYFEDLDKITTATT
                     PLKLSKKLFVETNTDRGPVITPVNDGDAIKVGDKIKVRVELRVDRDMEYVHMKDMRAS
                     ALEPVNVLSTYKWQGGLGYYESTKDASTNFFFSYLRKGTYVFEYTLFATHAGNFSNGI
                     TTIQCMYAPEFSSHSEGVRITVE"
BASE COUNT         2314 a         1808 c         1864 g         2756 t
ORIGIN      
        1 cgtaagtccg tattacaggg atgaaagcat gatacttgga ttaggattta ctccttacca
       61 tcaaatgctg caattattag ccaaaggcga tgatattttt accggaggcc gtgagtacta
      121 ttctcatccc aattaccggg ggaatgataa accaacaatg atacaccaaa gcagtgctac
      181 gggtatgcag gtgataccca ctacaggcat tgcacagggt attcagtacc tggaaagata
      241 taatgcttca ctacttaaaa aaggaataaa cggtgttttg ccggttacac tatgttcatt
      301 aggtgatgca agtgtaaccg aaggggaagt aagcgaagct tttcagttcg ctgtattaaa
      361 gcaattacca gttatttatc tggtacagga taataactgg ggtattagtg taacggcaca
      421 ggaagcaagg tctatgaatg cttttgagtt tgctgccggt ttcaaaggaa tgaaccgggt
      481 acaggtggat ggaagtgatt tcgaagcatc ttattctgta atgaaagagg tagtggattt
      541 tgtacgccgg gaaagaaaac cttatctcgt acatgcacag gttccgttgc ttggtcatca
      601 tacaagcggt gtaagaagag agttttacag aactgatgaa gactgggcca ggcaccagga
      661 acatgatcct aattcaaaac ttcggaaaaa attagttgag aaaggtgtac tggaaaatga
      721 attgctgcat atagaaaaag aagcggctga attggttgct ggtgattttg caaaagcagt
      781 tgcttctcct gaccctgatc cagcaacagt ggaagatcat atttttgttg caacacccat
      841 tacagaagag aaaggagaga ggagtcctgc aggggcagat aaagttataa tggtggatgc
      901 agcattattt gcaatcaggg aaataatgga gcaacatccg gaagctgtcc tgtatggaca
      961 ggacgtagga aaaagattgg gtggtgtatt ccgtgaagca gccacactag gggatatgtt
     1021 tggcgaacat cgtgttttta atacagctat ccaggaggct tatgtagtag gttccacggt
     1081 tggtatgagt gctgttggtc tgcggccaat tgtagaagtt caatttgcgg attatattta
     1141 tccgggtttt aatcaattgg tgacagaggt ttcgaagagt tgttacctta cctgcggtaa
     1201 atttcccgtg tccatgatat tgcgggtgcc tattggcgct tatggtggtg gtggtcctta
     1261 tcacagtggc agtgtagaaa caacattact ttccatcaaa ggaataaaag tggcctatcc
     1321 ttcgaatgcg gccgatatga agggattaat gaaagcggcc tattatgatc ctaacccggt
     1381 agtgatgctg gaacataaag gattgtactg gagtaaagtg cccggtacag aagacgccaa
     1441 aacaacagag ccgtcgaaag attatatcct gccatttggt aaggcaaata ttgtaacaca
     1501 ggcttctgcc aaagcaattg agaatgggga aagctgtgtg gtgatcactt atgggatggg
     1561 ggtatactgg gccaaagcgg ctacaaagaa ttttagccag caaattgaag tagttgacct
     1621 gcggacatta tatccacttg atgaagcact tgttttcgaa cgggtaaaat tgcacggcaa
     1681 atgtttagta gtaaccgaag agcaacagaa taattctttt gccgaagccc tggccggaag
     1741 aatttccaaa aactgttttc aatcattgga tgcacctgtt gatgtgatgg gtgcattaaa
     1801 ccttccggct gtacctatga atatgggatt ggaacaggcg atgttgccga atgcggagaa
     1861 agtatcagct cgtttaaagc aactgctggt ttcttaattt tttaaatcca gttttatctg
     1921 caattcttca aactgcttct catcaatcgt agcaggtgca tccagcatta catctcttcc
     1981 ggcattattt ttcggaaacg caataaagtc acggatgctt tcactccctc ctaaaatggc
     2041 acatagcctg tcgaaaccaa aggcaatacc accatgtggt ggggcaccgt attcaaaagc
     2101 gccgagtagg aaaccaaatt tatgttgctg ctcggctgca tccatgccca atgccgcaaa
     2161 cattttttcc tgcaattctc tttggaaaat acggatagag ccaccgccaa tttcattgcc
     2221 gttcagtacc atatcataag cattggcttt gatattagca taggggtgtt tcaggtattc
     2281 agcagcattc tcaataacag ggttgttatt gatcattgtt tcaatctgtg atggtttggg
     2341 tgaagtgaac ggatgatgac gggctaccca gcggttacct tcttcatcgt attcaaataa
     2401 cgggaagtcg agcacccaaa gcaatttgaa ttcagtatct tttcttaatc ccaaacggtt
     2461 ggccatttcc atcctcaggt cgctgatagc ttttcttgtt ctttcttctg cgcctgccag
     2521 cattaaaatc aaatcacctg cttttgcatt agccgcttct gctatggctt tcaaattttc
     2581 ttcgctgtaa aatttatcaa cactgctttt gtaagtaccg tccgtattta ctttaataaa
     2641 caccaggcct ttcattccta tctgcggacg tttaacccat tcggtcagtt cgtccgtttg
     2701 tttgcggctg tattcactgc aaccgggtac agcaatggcc aatacagttt cagcttcgtc
     2761 aaataatttg aagtcttttc cgcttatcaa ggttgagtta tcttgtttct cggtataaac
     2821 tgtagcaggt tttttcaggt tcagcacttt catgtcaaac cggatgtctg gtttatcatt
     2881 accataattc cacatggcat cttcccatgt catgcgttgt actgtctccg tataatcaat
     2941 tccctttact tccttaaaaa tggatttgat cataccttca aacatgttta aaatatcttc
     3001 ctgttccaca aatgccatct cacagtctat ctgggtaaat tccggttgac gatctgccct
     3061 taagtcttcg tcacgaaagc attttactat ctgatagtaa cgatcgtacc cgcttaccat
     3121 taataattgt ttgaatgttt gtggcgattg cggcaatgca taaaactgtc cggggttcat
     3181 tcttgaagga accacaaaat cccttgcacc ttccggtgtt gatttgataa ggaaaggggt
     3241 ttctatatcc atgaaattat gctggtgcag gtaattcctg gcagcacggt ttacagcata
     3301 acgcagttca agattccttt tcacagcgtt gcggcgcaga tcaaggaaac ggtatttcat
     3361 ccgcaggtca tcaccgccat cggtgtcatc ctgtatagtg aatgggggta cggcagatct
     3421 attcaacact ttaaaagtag aaaccactat ttcaatttca cctgtcggga tattaagatt
     3481 tttattgctt ctttcattca ccatgccggt tgcctgtatt acaaattccc ggcccagcgg
     3541 atttacttcc agttgattgt tcagttcttc gccaaataat aattgggtga taccataacg
     3601 gtcacgtagg tcaacaaagg tgatagcacc aaattttcta atggtttgta cccatccggc
     3661 cagtgtaact tctttgccat tattcccgat ccttaattcg ccacaggtgt gagttctgta
     3721 cattttttgt tcttttttct gaggcgcaaa gataaggcaa aagccccgtg agcaaagccg
     3781 gtactgtgtg ctacatcgtt gtcgttttct gaagttttat ttccctgggg cgttgtactg
     3841 aatagtaata gagcaatatg aaagccccga taaaaaaggg aatgagtgaa agttgtttac
     3901 ccgtaattcc accccagaat tctttttcat caaaccccag gaattcactg atcatccaga
     3961 atgaattggc actgatccaa aaggccacag ccaggttatg agctaactca gaggcgagct
     4021 tacgggttct ccaggctatc aggatagcca ctgttagggt agggacaatc attataattc
     4081 ccagcacttt ccatatcagg caccagctga tatcttttac cagccagaaa gcaatatgca
     4141 tattttccat ccggcgatag cgggacggta tgatgtactg gtcttcgttg ggttggttca
     4201 tcagtcagtt aattttgtaa aggaagttaa gaaatgttgc gggaaagtat ccggctgaaa
     4261 cgtttaatat cagcttccgg gtgtatggct tcatctctca ggatattatt ggtgagttgc
     4321 ctggcaaaga aaggagctaa ggaggagcct ttggttccca tcccgttcag gatgccaata
     4381 tttctataaa gaggatgaaa gccgacaaag ggccttcttt ccagcgtagc tggcctgatg
     4441 ccgcaaagat gttctgttat tgtaaaaggt atcttcagcc aatcttttaa aagctgctcc
     4501 gttttttccc tgaattcttt gccgggcttg tcattgtcaa attcccactg ataatttgat
     4561 cctacccacc aacggccggg agtaacaagc ggtgccagta tcattcccct tttataaata
     4621 tgctcatccg gcagccctgg aatttcagca atcaaaacct ctcctttatt gggagcaaaa
     4681 ggaagaagtt tgaagtaggg attattatta cttccggtgc catcacaaaa aataattgct
     4741 gacgccgtaa tagtatcgta ttggatctta cttggtgcaa ccgaaagttt attgatatcg
     4801 aatgcttctt ccagcagatt attagttttt tttaatcgtt gctgccaggc tggtatgatt
     4861 gtttcaaggt gggcagtgta aacagggttt attataccac atccaaaatc atagttaaaa
     4921 aacttattga aatgatcttg ctcccggtag tccgctacat actctccctt ttctgcaata
     4981 cgctgaaaaa aactttgccg catctgcggg ctgggaaaga aatcaataat attcttttga
     5041 gagatggccg taatagtaag ttcattcccg atccgggtat atgcttccag ggcgaaggga
     5101 agtatttctt ctgccatcca tacttctaca tgacggcggc cggttacagg attgataata
     5161 ccggcagcta gccgggatgg tgctttcttg tcgttattgt caatgacaag aaatgatttg
     5221 ttttcttttt gcagaaaata ggataaccat gttccggaaa tgccctggcc aacaattaag
     5281 aagtcaacat tcatggcgta aaagtaaaag aattagtaat tgacacttgc cggatattta
     5341 ttcaagggtt acttctataa aaccggagtt actcatcagt tttactttgt aaaaatcccg
     5401 gactggtaaa ttgtttataa gagagagcaa tacttttatt atgtatccgt ggcttactat
     5461 gacaatagtt tcacccgggt gtttttgttt tatatgtgat aaaaaattgt tcattctttc
     5521 tgccattgat tccacagttt ccattccttc gtacaaatca gattcgaggt aaggggaggg
     5581 gtatttctcc ccttgtaaaa tgccgagctg tctttccatt aaccggtcat ctgtaatgag
     5641 caaagagtgc ggagtatgtt gttgaatgga tttagctgtt tgaaaggccc ggttaagcgg
     5701 actggaataa attgtttgaa attggtattt tgttaactgt gcacccaatt tttcattctg
     5761 ccggattcca gtttcagata aaataccatg cgtttgtccc tggcaaatat tcaacccatt
     5821 ctcgatcgtt tcgccatgtc ttgctattat aagggttatc attctgactg caaagttgat
     5881 tatttatttt agtgaacagc cttgctaatt gacaaggctg tttatattat tcaacagtaa
     5941 tcctgactcc ttctgaatgc gatgagaatt ccggcgcata catacactga atagttgtaa
     6001 taccgttact gaaattaccg gcatgagtag cgaataaggt atattcaaag acataagttc
     6061 ctttccgcag gtaactgaag aagaaattgg tactggcatc tttggtactc tcgtaataac
     6121 ccaatcctcc ctgccatttg taagtactca atacatttac tggctccagt gctgaggccc
     6181 gcatgtcttt catatgcaca tattccatat ccctgtccac ccggagttct acccttactt
     6241 ttattttatc tccaacttta atagcatcgc catcatttac cggagtgatt actggtcccc
     6301 ggtcagtatt ggtttccaca aataatttct tcgataattt caatggagtg gtggcagtag
     6361 ttattttatc aaggtcttca aaatactgcc agtagatacc gccccaggag gccccccaat
     6421 cccctgccgg cagggactga gtttcctcta ccttaacagt tattttaccc atcaccggtt
     6481 gtactttatt tccttcaatc gttttcttaa agtaaccggt gccggcttca tctttgttat
     6541 tgttggaacg gataaccatg ttatcgagat tgatagtcac gacaggttct gttgccagcc
     6601 agttagttcc ctgtaataat aatgcataac aggcttctgc tgtggctttg gtagttttcc
     6661 agttattggt ttgtttattt tttaataacc atgttttcag gtcatctact gtttttgaat
     6721 ctttcgctac ttcctggaaa gtttctatca gtaatgcatg tgtttcaatt ggcatttcat
     6781 accagaacca gccataacgt tgatttttcc agtacatacc cagttcttca tttgttatgg
     6841 atgtttcttt cagcgatttt aaaatggctg ccggagtttt tacatcaccg gttctgtgca
     6901 gggccagcgc tgccattccc tgcatgtact tgctttgacc cacccatgtt tgctgcatcc
     6961 ggcttcggaa atacgtataa gctgtttgtg aagcttttgc aatctcatat tcaggaaaga
     7021 aacttctcat gtacaggtac tgaatactgc tatgtcccgg tgaatatttc ttcaggtcag
     7081 ttttgttttt aataagatta tcatagtctt cttttagttt tttatccagg tagggaatcg
     7141 ctgttgacag gatggcattc agtttggctt cctgcccggc agcaatgcct tttagttttt
     7201 taaggtgacc aataccggta acaatgtatt gtgtaatgta acgatcatcc ggtccgccgg
     7261 tgaaccaaac aaagccgcca ttgctgcttt gcatttgttt cagtttttca taggaactgt
     7321 taagttgctc actcattttc accatatcaa acagcagggc aatattcttt ttttgttctg
     7381 tttcattttt tgcctgcagg acccatggtg tttcttccag taacgcagct tttagttcct
     7441 ggttcttttg caggttactc atcagggcgg ctgtatcttt tatattccat tgttcaaaaa
     7501 cctgtttgat ctttggtgat gaattagcaa tatagccggc caatgaattg gcataataac
     7561 ggttccatgt ttgctccgca cagtcatacg ggtattccat caggtagggt agggcctgca
     7621 ctgcatacca ggcggggttg gaagtatatt caaccgttaa tgcatagtgt tgcaaggact
     7681 cactgctgcc gctgttcaat agtttttcaa aagagaagtc ctttgtaccg ctgccacgca
     7741 ttggtaatgg cattgtttca gtaacgagca tcctgttagt aagaacaggc atggcatttt
     7801 cttcgccatc actataattg ccggctttgg caacgattct ccaaaccagt gctttgttaa
     7861 agagataagg tacttctatc gggaatttca ccgcttcact ttgtcctgcg gccacggtga
     7921 aatactgatt agggaaagaa ttccggaacc agccatctac ggattgattg gtagtggcat
     7981 caaacaactg cagttcagcc gttcctgtca attctttatc agaaaggttg acgatcttgg
     8041 aactgaattc cattttatca ccttccctta agaacctcgg tgcattcggc tgtaccatca
     8101 gttctttttg tgtaacaatt tctttggtac tactgccaaa agctgcttct ttagtatgcg
     8161 ccagcgccat gaatttccac cttgtcagtg cttccggcat agtgaaagaa aattcaatca
     8221 tgccagtact gtcagttttc aggtcaggga aaaaaaaggc tgtttcgtta aagtttttac
     8281 ggattttgac atcattaccc tgtgtttgtt catcaaatcg aacaatcgaa tcaagcctga
     8341 cctctaatcc tgaaactttg cctgttaatg ccggttggat atttgcaact tcttttgctt
     8401 gtgtcatcgt ttcagcagat acggagttga ttcctcttaa agttattcgt tgatcagaaa
     8461 aaacgcctcc tctttcattg gaaagtagat agtcgtatcg tttatccagg gatttctgtt
     8521 cctgcattgc taagtatctt gaaacagatt cgcttttaat aaaattttga gtactgttcc
     8581 agctgctcat tttatagtta ttataccaga tataaggcct gctccatcca tggggataaa
     8641 actggtcaag tgatgcatca tacatactgg ccagtagttc tgcagctact ttttcatttt
     8701 tataaccgct aatttttagt gtccattttt cttcactgcc gg
//
LOCUS       DAORTI010000138         8231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_9659_length_8231_cov_5.904477, whole genome shotgun sequence.
ACCESSION   DAORTI010000138 DAORTI010000000
VERSION     DAORTI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_8231_cov_5.904477"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..2725
                     /locus_tag="PLO70_13915"
     CDS             245..2725
                     /locus_tag="PLO70_13915"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQZ75613.1"
                     /translation="MRYIFLTFSLCFFISYLHAGKIAGIITDTEGKILPFASVFIKGT
                     NKGTNANSEGRYSITLAPGQYTLVCQYVGYKKEEKNIIVGPDDLKVDFKLTLQEMMLG
                     EVILKNGEDPAYEIIRQAIKKRKYHQGQLDKFQCEVYTKGQLRVRSYPKKLFGQKVDF
                     EDGDTSKQKMLYLSETVSNYYADKPNKEKVEVISSKVSGQSDGLGLAAPRFFSLYDNN
                     ISIGSNLNPRGFISPISDNALNYYRYKLEGTFYEDGKQVSRIKIVPKRKFEPLFSGYL
                     SIVEDDWLIHSADLVLTKQSQMEQLDTLRLVQLFRPVSRGNWFIASQVIYPAVKILGF
                     DAYGSFINIYSNVNTNPEFSKKIFDNTILKYTDSSSKKTVDYWEKTRPVPLMQDELKD
                     YKRKDSLEIVRKDPRYLDSISKVRNKVTVMNVLLLGQTFQSQRKRTTLTFIPLFELVN
                     FNAAEGLVINPAFTWSKRLDTITYSRRSISISPSLRYGFANGHFNGHATFRYNYGKQY
                     ANNISLSGGKRVFQFNNNSPIGERGNTLSSLLSEENRIKSYEAVYLRGSYRISLGQGF
                     LITAGFQYQVRRPLENRTNYTWRDRADKEYTPNYPNEIVNENISPHKVFYSLLGINWQ
                     PGARYIELPDRKINIGSKYPVFSLQYIRSFDGFLGSDADFSKWRFSVSDNINLKLRGL
                     FRYRLSMGGFIDNDKVQLPDYNHFNGNISTFATEYLNSFQLLPIYQYSNTSEFYSLAH
                     LEYNLKGFLTNKIPVFRRLNFYLVTGANGFYINGDKNYYEWFVGFDNIFKQIRIDFVQ
                     SYQNGRQWQNGFRVGLSRFRGQRGDDWP"
     gene            2903..3934
                     /locus_tag="PLO70_13920"
     CDS             2903..3934
                     /locus_tag="PLO70_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQZ75614.1"
                     /translation="MAQLHNRISRRELKDRILNDPTPRTTISFYCYFKIDDPAVFRNI
                     LYKDLKEIGVLGRIYVANEGINAQISVPAENVDRFKAYLYSMEPLNGLRLNIAVDDDG
                     KSFFVLDVKVRKKIVADGIEDPAFDMANKGKYVNAEQFNQLTEDPDTVVIDMRNYYEY
                     EVGHFQNAIEVPSDTFREQLPMAVDMMKDKKGANIIMYCTGGIRCEKASAYMLHQGFK
                     NVFHLEGGIINYARQIKEKGLKSKFIGKNFVFDERLGEKITDDIIAHCHQCGKPADTH
                     TNCKNDGCHLLFIQCEDCAKTYEGCCSPECQETLHLPAERRKELRKGIDKGQNIFNKS
                     RERVRPKIK"
     gene            complement(3952..5073)
                     /locus_tag="PLO70_13925"
     CDS             complement(3952..5073)
                     /locus_tag="PLO70_13925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HQZ75615.1"
                     /translation="MKYFLTLLCFAYFYLQSPAQEHYLVVGTYDSPESEGIYIYTFNS
                     STGNAEKVSHIKSSNPSFVAISPDEKFVYAVFENSKDGNGGEVAAYSFDRQTGQLRFI
                     NKQLSGGDHPCYVETDKTGKWVFAGNYSSGNLAVFPVNTDGGLSAAISTIQHSGSGKN
                     FQRQKSPHVHSTVISPDNNWLFAPDLGIDKIMIYRFDANSGQLTAGDQAYAVSEPGSG
                     PRHFTFHPNNKYAYLIEELSGTVVTYKYKKGKLNTIQRLSTMPSGDTSFAGSADIHVS
                     PDGKFLYASNRSNANTIAIYSVNKKGKLSLIGHQSTLGKGPRNFNFDPSGSFLLVGNQ
                     YSDEIVIFKRDATSGLLSDSGKRIAVGKPVCIKWISAAR"
     gene            5211..5630
                     /locus_tag="PLO70_13930"
     CDS             5211..5630
                     /locus_tag="PLO70_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75616.1"
                     /translation="MKRIFTLSVTVLLTAVFLSSCVRESLPHNNENYWLSKEMGEVVY
                     SDSYCNYYVVETYDGFTIIRSYGGYKPYEGSIVYGNFSSPGTKDMYNRSSGIVFTGTV
                     TDYWLSYTDAQYALDYYCPLYGKNQVREFKKSTVIKK"
     gene            complement(5693..6622)
                     /locus_tag="PLO70_13935"
     CDS             complement(5693..6622)
                     /locus_tag="PLO70_13935"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQZ75617.1"
                     /translation="MAVFSFPNQTAFYKGKVRDVYSIANKWLVMVASDRISAFDVILP
                     RPIPYKGQVLNQIAAYMLNATRDICPNWLVDVPAPTVSIGQKCEPFKIEMVVRGNLTG
                     HAWRTYTSGKRDLCGASMPDGMQENDYFPSPIITPSTKAAEGHDEDISPEEIIKTGLA
                     TPEEWEILSEYALRLFERGKKIAAKQGLILVDTKYEFGKIGDSIYLMDEIHTPDSSRY
                     FYADGFEERQAKNERQKQLSKEFVREWLIANNFMGKQGQTVPEMSDEWVNTISKRYIE
                     LYEKVIGAAFIPEKLTDEEIYTRVVSSITALNK"
     gene            6772..>8231
                     /locus_tag="PLO70_13940"
     CDS             6772..>8231
                     /locus_tag="PLO70_13940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQZ75618.1"
                     /translation="MRKSYRYLASSLMAVLFTIAAYAQTVTITGSVRNNTTKESVPAV
                     SVSVKGTTQGTYSNSDGEFSLRVSKLPVVLVFSSVGYEDQEITVSDASKPFNIDLKIN
                     NALGQEVVVAATRSPTRLLESPVTVERMSNNVLRNVAAPNYYEAIGNLKGVDVHTASL
                     TFRTVTTRGFVSSGNTRLNQLIDGMDNQAPGLNFSVGSVLGPSDLDVDNVEILSGASS
                     ALYGSGGMNGTILISTKNPFKYQGLSYNIKQGIMHVDGKQRNASPFYDWSFRWAKAYK
                     NKLGIKLAAQIVKGADWEAEDYRNKQQIGILSKVVGGNRQNDPNYNGINMYGDETSAD
                     MNGFSYFVQDQTRRSILAATGGAFDVVNAANGYFTAIGNPAYPTNAQIGAFIGGLGFL
                     GSAGQAAVQQMMPFYIGLRNGYFNKASVSRTGYEEQSLVDYNTINFKFTGGLHYKIND
                     RLEASFNTYMGTGTTVYTGADRYSLRNLKMAQHKVE"
BASE COUNT         2454 a         1509 c         1762 g         2506 t
ORIGIN      
        1 gtggatcgat atgaaaaata atggagtcct ttcacaaaaa attgcatcgg tactcccttt
       61 gggtggtaat tcaatattac tgattgtggt tacaggagtt gcaggtggat tggtggccgg
      121 tttcgcagcg atgagtggca gttaccttcg ttcttcagca aaagactgat gttaaagctt
      181 ttctaacagt agtaattaca ttggtaaaga aatttactgc atctatcttg tggataattg
      241 aattatgagg tatatttttt taactttttc cctttgtttt tttatttcgt atttacatgc
      301 tggtaagata gccggtatca ttacagatac agaagggaaa atattgccct ttgcctctgt
      361 ttttataaaa ggaactaaca aaggaaccaa tgccaatagt gaaggccgct attccatcac
      421 acttgctccg ggacaataca cattggtttg ccaatatgtg ggatacaaaa aggaagaaaa
      481 aaatataata gttggtccgg atgatctgaa agttgatttc aaacttacat tgcaggaaat
      541 gatgctggga gaggtgatac tgaagaatgg tgaagatccg gcctatgaaa ttatacgaca
      601 ggcaataaag aaaagaaagt atcaccaggg acaattggac aagtttcaat gtgaagttta
      661 taccaaagga caattgcggg taaggagtta tccgaaaaaa ttgtttggtc agaaagtaga
      721 ctttgaagat ggagacacca gcaaacaaaa gatgctgtac ctgtctgaaa cggtctctaa
      781 ttattatgct gacaaaccca ataaagaaaa agtggaagtg atctcctcta aagtaagtgg
      841 tcagagtgat gggttgggac tcgccgcacc tcgttttttc tctttgtatg ataataatat
      901 ttctattggc agcaatctca acccgagggg gttcatttct ccaatctcag ataatgcact
      961 gaattattac aggtacaaac tggaaggtac tttttatgag gatggaaaac aggtcagtcg
     1021 tataaaaata gtacccaaaa gaaagtttga gcctttattc agtggttatt taagcatcgt
     1081 ggaagatgat tggttgatcc actctgcaga tttagttctc acaaaacaat cgcagatgga
     1141 acaactggat acattgagac tggttcagtt attccgaccg gttagtcggg gaaactggtt
     1201 tattgcaagc caggtaattt atcccgccgt taaaattttg ggatttgatg cgtacggtag
     1261 ttttataaat atctattcca acgtcaatac caatcctgaa ttcagcaaaa agatctttga
     1321 caacacaatc ttaaagtata ccgatagttc cagtaaaaaa acagttgatt attgggaaaa
     1381 gacaagacca gtgccgttga tgcaggatga attgaaagat tataagcgaa aggatagtct
     1441 tgaaatagtc cggaaggatc cccggtattt ggattctata agtaaggtcc gtaataaagt
     1501 aaccgtaatg aatgtgttat tattagggca gacttttcaa tcacaacgaa agcgaactac
     1561 attaaccttt attcctcttt ttgaactggt gaatttcaac gcagcggagg gtttggtgat
     1621 taatcctgct tttacatgga gcaagcggct tgatacaatt acttatagcc gccggagtat
     1681 atccatttcg ccttccttgc ggtatggatt tgcaaacggc catttcaatg gacatgccac
     1741 cttccggtat aattatggca aacaatatgc aaacaacatc agcttatccg gggggaaaag
     1801 agttttccag tttaacaaca acagcccgat aggagaacgg ggaaatacac tttccagttt
     1861 gctaagtgag gaaaacagga tcaagagcta tgaggccgtt tatttgcgtg gcagttatcg
     1921 gataagttta gggcagggat ttttaataac agccggtttt cagtaccagg tcagaagacc
     1981 acttgaaaac agaacaaatt atacatggcg tgacagagca gataaagagt atactcccaa
     2041 ttatcccaat gaaatagtga atgagaatat cagtccgcat aaggttttct acagtttgtt
     2101 agggattaac tggcaacccg gcgcccgtta tatcgaatta ccggatcgta aaattaatat
     2161 tggttcaaaa taccccgttt tctctcttca atatatccgg agctttgatg gattcctggg
     2221 cagtgatgct gatttcagca aatggcggtt ttccgtatct gataatatca atttgaaact
     2281 tcgcggattg ttccgttaca gattaagtat gggtggtttt attgataatg ataaagtaca
     2341 gctgccggat tacaatcact tcaatggaaa tatttccact tttgcaacag aatatctcaa
     2401 tagttttcaa ctgctgccga tttaccaata cagtaatact tctgaattct attcgctggc
     2461 ccatcttgaa tataatctaa aaggattttt aacgaataaa attcccgttt tcaggaggtt
     2521 gaatttttac ctggtaacag gtgccaatgg tttttatatt aatggggata agaactacta
     2581 tgagtggttt gttgggtttg ataatatatt taaacaaata cggattgact ttgtacaatc
     2641 ataccagaat ggcaggcaat ggcaaaatgg attccgggtt gggttaagcc ggtttagggg
     2701 gcagcgggga gatgattggc cctgatgttt tagtatcaaa tctgaagggg atttttttac
     2761 ccatatattc cggggaaatg gtaattgata ttttctttat cacttttaca tgttacgctc
     2821 ctggcttatc tttgcactgc tttaagaatt ttgcccaacc tgcaattggg attctgcaag
     2881 cacaggatta aaattcatac agatggcaca gttacataac cgtatctccc gcagggagct
     2941 gaaggatcgt atactcaacg accctacacc aaggaccacc atttcctttt attgctattt
     3001 taagattgat gacccagccg ttttcagaaa tatactgtac aaagacctga aggaaattgg
     3061 cgtcttgggg cggatctatg tggcaaatga aggtatcaat gcacaaatta gtgtaccagc
     3121 ggaaaacgtt gatagattta aagcttacct gtattcaatg gaacctttaa atggtttgcg
     3181 tttgaatatt gcagtagatg atgatggtaa atcttttttt gtattagatg taaaagtgag
     3241 aaaaaaaatt gttgctgatg gaattgaaga ccctgctttt gatatggcca ataaggggaa
     3301 atatgtcaat gcagaacaat tcaatcaact tacggaagac ccggatacgg tggtgataga
     3361 tatgcgtaac tattacgagt atgaagtggg tcattttcaa aatgctattg aagtgccttc
     3421 tgatactttc agggaacagt taccaatggc ggttgacatg atgaaagata aaaaaggtgc
     3481 caatataatc atgtattgta ccggaggtat ccggtgcgag aaggcttctg cttatatgct
     3541 ccaccaggga tttaaaaatg tctttcacct ggaaggaggt atcattaact atgcccgtca
     3601 aataaaggag aaaggcctca aaagtaagtt cattggaaag aattttgttt ttgatgaaag
     3661 gctgggagag aagattacgg atgatatcat tgctcattgc catcaatgtg gtaagccggc
     3721 tgacacacat actaattgta agaatgatgg ttgtcacctg ctttttattc aatgtgagga
     3781 ttgtgcgaaa acttatgagg gctgttgtag cccggaatgc caggaaactt tacatcttcc
     3841 ggcagaaaga agaaaagaac ttagaaaggg aattgataaa ggacagaata tttttaataa
     3901 atcaagagaa cgggttcgcc caaaaattaa ataagccaga ttctgccaca tttacctggc
     3961 agcagagatc catttgatgc aaacaggttt accaacagcg atccttttgc cgctatccga
     4021 taataagcct gatgttgcgt ctcttttgaa aattacaatt tcatcgctat actggttacc
     4081 taccaataaa aaacttccgg aaggatcaaa attgaaattg cggggtcctt taccaagtgt
     4141 tgattgatga ccaattaaag aaagtttccc ttttttgtta actgaataga tagcgattgt
     4201 atttgcgtta ctgcggttag aagcatacag aaattttccg tcgggagaaa catgtatatc
     4261 agcacttcct gcaaaactgg tatcaccgga cggcatagta cttaaacgtt gaattgtatt
     4321 cagctttcct tttttgtatt tataggttac aactgtgcca ctgagttctt caatcaggta
     4381 agcatattta ttgtttggat gaaaagtgaa gtgcctgggg ccggaacctg gttccgatac
     4441 tgcataagcc tggtcgccag cagttagttg tccactgttt gcatcaaatc tatagatcat
     4501 tattttatcg ataccaaggt caggtgcaaa aagccaatta ttatccggtg agattactgt
     4561 actgtgtaca tgcgggcttt tttggcgttg aaaattctta cccgaaccac tgtgctgaat
     4621 tgttgaaatg gctgcggata gcccaccatc agtgttaacg ggaaaaacag caaggttgcc
     4681 gctgctgtaa ttgccggcga aaacccattt tccggttttg tcagtttcaa cataacaagg
     4741 atggtcacct ccggatagtt gtttgttaat aaatctaagc tgcccggttt gccgatcaaa
     4801 tgaataagcg gccacttcac cgccattgcc atccttacta ttctcaaata cggcatatac
     4861 aaatttttca tccggggata tggcaacgaa tgacggattg gaagatttta tatgactgac
     4921 tttttccgca ttgccagtac tgctattgaa tgtatatata tagatacctt cactttctgg
     4981 tgaatcatat gtccctacaa ccaggtaatg ttcctgtgct ggtgactgca gataaaaata
     5041 agcaaagcac aaaagtgtca ggaaatattt catttttagt ttttattaag aagtgaattt
     5101 ccttctttca tttcatttta acaaacgctt atatatcctt tttgattctg gcacagtctt
     5161 attatagtat aaagtggcaa caacaatctt taaactttaa atcaaaagcc atgaaaagaa
     5221 tttttacact ctctgttacg gtactattaa cagctgtttt tttgagcagt tgtgtaaggg
     5281 aatcattacc tcataataat gaaaattact ggctctccaa agaaatgggt gaggtcgttt
     5341 acagcgattc ctattgtaat tattatgtag tggagactta tgatggtttt actattatca
     5401 ggtcttacgg cggttataag ccttatgaag gaagtatcgt atatggaaat ttcagcagtc
     5461 ccgggacgaa ggatatgtac aaccggtctt ccgggatcgt atttacagga acagttactg
     5521 attactggct gagctatacg gatgcacaat atgcactgga ttattattgc ccgttatatg
     5581 ggaaaaatca ggtaagagaa tttaaaaaat caacagtcat aaaaaaataa tgcttgtcgg
     5641 tgataagcac gggacgggaa agggaaatgg gaataatgat tcctgtttcc ctttatttat
     5701 ttagtgctgt tattgaagat actacccggg tatatatttc ttcatcggtt aatttttcgg
     5761 gtataaatgc tgcgccaatc actttttcat acagttcaat atatcgtttg gagatagtat
     5821 tcacccattc atcgctcatc tcaggaactg tttgaccttg tttgcccata aaattgttgg
     5881 caatcagcca ctctctcaca aactctttac tgagttgttt ttgccgctca ttttttgcct
     5941 gtctttcttc aaaaccatca gcataaaaat atcttgagct gtcgggggta tggatttcgt
     6001 ccatcaggta aatgctgtca ccgattttgc caaattcata tttggtatct accagtatca
     6061 acccttgctt ggctgcgatc tttttccccc tttcaaaaag tctcaaagca tactcactta
     6121 atatttccca ttcttcgggt gttgctaacc cggttttaat gatttcttca ggggagatat
     6181 cttcatcatg cccctctgct gcttttgtgc tgggagtaat aatggggctt gggaagtaat
     6241 cattttcctg catgccatcc ggcatggaag ctccgcaaag atcccgtttt cccgatgtat
     6301 aggttctcca ggcatgcccg gttaggtttc cccttactac catttcaatt ttgaagggct
     6361 cgcatttttg gccaatagaa acagtagggg cgggcacatc taccagccaa ttgggacaga
     6421 tatcccgggt ggcattcagc atgtaagcag caatttgatt cagcacttgt cctttatagg
     6481 gaatcgggcg gggtagaata acatcgaaag cggatatacg gtcagatgcc accattacca
     6541 gccatttgtt ggcaatgcta taaacatcac gaaccttgcc cttataaaat gccgtttgat
     6601 ttgggaatga aaaaactgcc atttttcgaa agtaagctgc ccttaaaaac caaccaatcc
     6661 ggagtccccg aagatttcaa caatttatcc tttcccctta aaaattaaat ttttagataa
     6721 actttacaat gcttattttt ccgtcaaatt ccaaaaccaa aattccctat tatgagaaaa
     6781 agctaccgct atttagcttc ctcgctgatg gctgttttgt ttacgattgc tgcttatgct
     6841 caaacagtta ccattacagg ttctgttcgt aacaatacaa ctaaagaaag tgtaccggct
     6901 gtatctgttt cggtaaaagg taccacccag ggtacttatt ctaattcaga tggtgagttc
     6961 agccttagag tttccaaatt acctgttgta cttgtattta gctcagtggg ttatgaagat
     7021 caggagatta ctgtttctga tgcctccaaa ccgttcaaca ttgatttaaa aatcaataat
     7081 gcgttgggcc aggaggtagt agtggctgct acccgtagcc cgacacgatt gctcgagtct
     7141 cccgttaccg ttgaaagaat gagcaacaac gtgttgagaa atgtggctgc tcctaactat
     7201 tatgaagcaa ttggtaacct gaaaggggtg gatgtgcata ctgccagctt aaccttccgt
     7261 acggttacta cccgtggatt tgtaagcagc ggtaataccc gtttgaacca attgatcgat
     7321 ggtatggata accaggcgcc cgggttaaat ttttctgtgg gtagtgttct gggcccgtcg
     7381 gatcttgacg ttgataatgt tgaaattctg tcaggtgcat cttctgcttt gtatggttca
     7441 ggcggtatga atggaaccat acttatctct acaaaaaatc catttaaata ccagggtctt
     7501 agttataata tcaagcaggg aatcatgcat gttgatggaa aacagcgcaa tgcttcaccc
     7561 ttctacgatt ggtctttccg ttgggcaaag gcttataaga acaagctagg tatcaaactg
     7621 gccgcacaaa ttgtaaaagg tgctgactgg gaagctgagg attatcgcaa taaacaacag
     7681 atcggtatcc taagtaaagt agtcggtggt aatcgtcaga atgatcccaa ttataatggt
     7741 atcaatatgt atggtgatga gacaagtgct gatatgaacg ggttttctta ttttgttcag
     7801 gatcaaacaa gaagaagtat tcttgctgca acgggtggtg catttgacgt tgtaaacgct
     7861 gcaaatggtt acttcactgc tattggtaat ccggcttacc caacaaatgc tcaaataggg
     7921 gcctttattg gtggacttgg ttttctgggt tctgccggac aagctgctgt tcaacaaatg
     7981 atgccttttt atatcggttt aagaaatggt tattttaata aggcaagtgt ttccagaaca
     8041 ggatatgaag agcaatccct ggtagattat aatactatca atttcaaatt taccggcggg
     8101 cttcattata aaataaacga caggctggaa gcttctttca acacttatat ggggaccggt
     8161 actactgtat atacaggagc agaccgttat tctctgcgca acctgaaaat ggcacagcat
     8221 aaagttgaat t
//
LOCUS       DAORTI010000139         8203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_9707_length_8203_cov_10.021846, whole genome shotgun sequence.
ACCESSION   DAORTI010000139 DAORTI010000000
VERSION     DAORTI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9707_length_8203_cov_10.021846"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLO70_13945"
     CDS             complement(<1..812)
                     /locus_tag="PLO70_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQZ75619.1"
                     /translation="MAVYLNDQFLNNDQAQLQVSDLSMQRGYAIFDFFRTVNGVPLFI
                     DDHLDRFYASAAAMHLPMNKSREELKHIVQELLHRSSLAEAGIRLLLTGGYAADSYSI
                     ASPNLVITCNPVKTATPDDFEKGLLVLTYEHQRELPHIKSINYIMALLLQPLLKEKRA
                     DDVLYHNKESITEFPRCNVFIVTKENKLVTPARNMLRGITRKNVLALAATSIQVEERD
                     ISIAELFSASEVFLTSTSKKILPVLKINDSNIGNGRPGPVTTALYRQFIALE"
     gene            complement(814..1944)
                     /locus_tag="PLO70_13950"
     CDS             complement(814..1944)
                     /locus_tag="PLO70_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQZ75620.1"
                     /translation="MRLPVLLILLLLSFQLPAQSNKRAADLNIHIGVMQPGKLNAITD
                     VSGVTVGHTTLIKADSVRTGVTVILPHGGNLFQQKVPAAVYTGNGFGKLAGSTQIKEL
                     GNIESPIVLTNTLGVATAMNAIVSYTLSLKGNENVQSVNAVVGETNDGYLNDIRGRHI
                     KEQDVLNAIHNASPGKLTEGNVGAGTGTVCFGFKGGIGTASRVLPKKLGGYTVGVLVQ
                     TNFGGVLTVDGVPVGEELKKYYLRDQLNETPDGSCLIVVATDAPLDARNLERLAKRAF
                     MGLARTGGIASNGSGDYVIAFSTDSTLRIPSDGREKLTQTIVANDAMSPLFMAAIEAT
                     EEAIINSLLAAATMKGKNGRIVEALPPDKLKEILLKYKRSTD"
     gene            complement(1989..2348)
                     /locus_tag="PLO70_13955"
     CDS             complement(1989..2348)
                     /locus_tag="PLO70_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HQZ75621.1"
                     /translation="MKLDLATFRPLNVQRAREGFKCYDNQPLTYWTTALAGEVGELCN
                     MIKKMQRVEKGGLDGGSSYTAKDINKEMLKEEIGGIAIYLDLLASLLDISLEEAITDT
                     FNSKSEKYGFSQFVKPL"
     gene            2958..4001
                     /locus_tag="PLO70_13960"
     CDS             2958..4001
                     /locus_tag="PLO70_13960"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQZ75622.1"
                     /translation="MPSVKAYAAQSATSPLDQWTIERRDPKPHDVQIQILYCGVCHSD
                     LHFARNDWGMTVYPIVPGHEIVGKVTAVGSHVKNFKVGDLAGIGCLVDSCRECDNCKE
                     GLEQYCNNGFVGTYGGMEKDGSGATHGGYSKSILAHEDFVLHISDKLPLEAVAPLLCA
                     GITTYSPLRHWKVGKGTRVGILGLGGLGHMAVKLAAAMGAEVTMLSHTASKEADAKRL
                     GAQKFVLTSSEEQVKGVTGYFDFILDTVSADHDYNFYLSLLRSSGTMVCVGVPSAPAV
                     VPAMNLIFGRKSIAGSLIGGIPETQEMLDFCAEHGIVSDVEIIAMRDINEAYERMLKS
                     DVKYRFVIDMSTL"
     gene            complement(4220..4915)
                     /locus_tag="PLO70_13965"
     CDS             complement(4220..4915)
                     /locus_tag="PLO70_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75623.1"
                     /translation="MKKLLLMTIVLLVSGTILAQGSAKTTATATKSVLAFHAGPSFPV
                     GDFASNDFDNEQAGFAKTGFTIDLNYGYQFHPNAGIAASVFYNKYNMHNVVMDIVLEG
                     RLNEEVTLDIMDNWQFYGITAGPMLTFGLAKNIWTDLRLMGGVVNTKSPKISYEGELL
                     EKAHWSIAPALQGGLDLRFGTGKNMFIFTNLDYLYMKPEFTASSSEWFDEGEKFKQKI
                     SVLNLTGGIGIKF"
     gene            5233..5823
                     /locus_tag="PLO70_13970"
     CDS             5233..5823
                     /locus_tag="PLO70_13970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQZ75624.1"
                     /translation="MSFRLLFLYLLTGIAATAQQQYSTADLKNGKFKDDSSYIYSLPF
                     ETGKKVFLAQGYESMFSHKSSKALDFKVKTGTKVCAARSGIVVAARKDSDRRGLKQEN
                     LSDGNYIFIRHDDASVAQYWHFRKDGVLVKVGDSVTTGQLIGFSGNTGYSAFPHLHFE
                     VRGFDAGGNETDLPTRFYTQRGVIYLQPGKFYRTRR"
     gene            5886..6404
                     /locus_tag="PLO70_13975"
     CDS             5886..6404
                     /locus_tag="PLO70_13975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQZ75625.1"
                     /translation="MIVVYILAALIVILLIVAALMPKIYNVEKNIIIKCSVTDVMNRI
                     SDMNYYSQWNPWQQSDPTTKSTITGTPGTTGHKYAWEGKKVGVGSLTLLSMNHKHIHF
                     ELEFLKPWKSKAKDNWLFEPWGDHNETKVTWQNSGNLPWPIARLIGPMLNKNLNQQFE
                     QGLNNLKKMCEG"
     gene            complement(6493..7164)
                     /locus_tag="PLO70_13980"
     CDS             complement(6493..7164)
                     /locus_tag="PLO70_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQZ75626.1"
                     /translation="MKRIKIFSTPHKALRKLMAEFSLLAGRTDYANESELHVLKQTGN
                     EMFDLLKEHAHIEETIILAVLETKAPGSSEENTEEHELLEKVQEELETWLNSFDGSQP
                     DEEGFQFYLAFSSYHGQYLEHIIHEETTTQELLWKYFSEEELMGLHMQILKSIPFEIF
                     LVWGKHVLPAQTRKENLAMLTGMKMMLPAPAFEKMVAVLQASVSPNEYYELMNELEKL
                     PAKAA"
     gene            complement(7222..7800)
                     /locus_tag="PLO70_13985"
     CDS             complement(7222..7800)
                     /locus_tag="PLO70_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQZ75627.1"
                     /translation="MKKFHLSAVLMMAAAIGAFTACKQGSSQSVQTTISKDSLVKRGN
                     YLVTTMGCNDCHSPKVMTPMGPALDTTRLLSGHRSDMSLPPANNEALKNGWALFYTEG
                     TAMVSPMGTSYAANITSDATGIGNWSLQQFKTALTRGKSKGIETNRDLLPPMPWENYK
                     QLSEEDITAIFTYLQSTKPVKNIVPEPQLATR"
     gene            complement(7938..>8203)
                     /locus_tag="PLO70_13990"
     CDS             complement(7938..>8203)
                     /locus_tag="PLO70_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75628.1"
                     /translation="GLPIPEIKLERAGASAVVLANKSAEQFIITGIEEALAEKQTDVR
                     IFGKPATRPYRRMAVALVYEDINADINELRKKAKAAADKIKIE"
BASE COUNT         2223 a         1759 c         1727 g         2494 t
ORIGIN      
        1 ctctccaacg caataaactg ccggtaaagt gccgtggtta caggtccggg ccggccatta
       61 ccaatattgc tatcattgat ttttagtacc ggcaatatct ttttactggt gctggtaaga
      121 aatacttcgg aggcagaaaa taattcagca atggaaatat ctctttcttc cacctgtatg
      181 ctggttgccg ccaatgcaag tacattttta cgggtgatgc ccctgagcat attacgggca
      241 ggtgttacca gtttattttc ttttgttaca ataaacacat tgcaacgggg aaactccgtg
      301 atactttctt tgttgtgata caacacatca tctgctcttt tctcttttaa taaaggctgc
      361 agcaacaacg ccattatata attgatgctt ttgatatgcg gcagttcccg ctggtgttcg
      421 taagtgagca ccaataaacc tttttcaaaa tcatccgggg tggccgtctt cacaggattg
      481 caggtaataa ccaggttggg tgatgcaata ctataactat cggcagcata accaccggtg
      541 agcagtaaac gaatgcctgc ttctgctaat gatgaccggt gcagtagctc ctgcacaata
      601 tgtttcagtt cttccctgct tttattcatt ggcaggtgca tggcagccgc agatgcataa
      661 aagcggtcga gatgatcatc gatgaacaac ggtaccccat ttaccgttct gaaaaaatca
      721 aaaatggcat agccccgttg catggaaaga tcagacacct gcagctgcgc ctggtcattg
      781 ttcagaaatt gatcgttaag ataaacagcc atattaatca gtactgcgtt tatattttaa
      841 taatatctcc tttagtttat cgggcggcaa tgcttccact attcttccgt tctttccttt
      901 catcgttgct gctgccagca gtgaattgat gatggcttct tccgtggctt ctatcgccgc
      961 catgaagagt ggtgacatcg catcatttgc tacaatagtt tgtgttagct tttcccttcc
     1021 gtcagaagga attcgaaggg tactatctgt agaaaaagca atgacataat caccgctgcc
     1081 attactggca ataccacctg tgcgggccag tcccataaat gctcttttgg ccaggcgttc
     1141 aaggttgcgg gcatccagcg gtgcatcggt agccactacg atcaaacaac tgccatccgg
     1201 tgtttcattc agctgatcac ggagataata ttttttcagt tcttctccca ccggcacacc
     1261 atctaccgtc aacactccgc caaaatttgt ctgcactaat acaccgacag tgtagccacc
     1321 cagttttttg ggtaatactc ttgaagccgt tccgatccct cctttgaaac caaaacaaac
     1381 cgttcctgtt ccggcaccca cattaccttc cgttagtttt cccggtgatg cattatggat
     1441 cgcattcagc acatcctgtt cttttatatg tcttccgcgg atatcgttca ggtaaccatc
     1501 attggtttct cccaccacgg cattcaccga ctgtacattt tcatttcctt ttagtgataa
     1561 cgtataagaa acaatggcat tcattgccgt ggccacgcct aatgtattgg tgagaacaat
     1621 gggtgattcg atatttccca attcttttat ttgtgtggag ccggccagtt ttccaaaacc
     1681 attacctgtg taaaccgcag ccggtacttt ctgctgaaaa agatttccgc catgcggcag
     1741 aatgacggtg acaccggttc ggacagaatc ggcttttatt aaagtagtat gccccacggt
     1801 tacaccgctt acatccgtga tagcattaag ttttccgggc tgcatcaccc caatatggat
     1861 atttaagtcc gccgctcttt tattggattg tgccggcaat tgaaaggaaa gtaacaacag
     1921 gataagcaat acaggcagcc gcattttttt caggaaagat agggtttctg ctgaaactgt
     1981 ttttatgatc acaggggttt tacaaactgg ctgaaaccat atttttctga cttactgttg
     2041 aaggtatcag taatagcttc ttcgaggctg atatcaagca gtgatgccag caggtcgaga
     2101 taaatagcaa tgccgccgat ctcttcttta agcatttctt tattgatgtc tttggcagta
     2161 taactgctgc cgccatccaa acctcctttt tccacccgct gcatcttctt gatcatgtta
     2221 caaagttctc ccacttcacc ggcaagtgct gttgtccagt aggtgagggg ctggttatcg
     2281 taacatttaa atccttccct ggcccgttgt acattgagcg gtctgaatgt ggcaagatca
     2341 agtttcattc tggtaatttt agtgcagtac taaaattaca ggtcttgttg ttgtgaaagg
     2401 tataaaccaa ggcaaaaaaa tctcccggct tacgggccgg gagactcaat cgcttcaccc
     2461 ggtgtacttc tgtaatgaat ccttttcatt acgtcctgta cccttgagca aatctattct
     2521 ttagtttctc atgtgtagaa ataaaagtaa tcggcagatc acactgcccc tgtttgcttc
     2581 ttgtacaggt agttcaccac ggtgaacatg ttactgtttg caaatgtccc gttctgatct
     2641 tctgactaac cggaaggaaa tcccggttct tctgatcggc cgcatttcat ttgcaccgtg
     2701 gtaaaataag ggcaaaaatc atcacctatc aatgattaaa tactgtactt tctttgagag
     2761 ttgtcagcag aatttcattt aatatgttat acctttatag atagtcattt ttcaagcatt
     2821 cgctgctacc tgtcagtcaa tagtgattac ctatattctt tcctttctta ttaccgttgc
     2881 tttttcctgc acaataattt tgaaattcat tcccttttaa taaattacat ttaccaacta
     2941 taaaaaaata aataattatg ccttctgtaa aagcctatgc tgcccaaagt gctacttctc
     3001 cattggatca atggaccatt gaaagaagag atcccaaacc acatgatgta cagatacaaa
     3061 tattgtattg tggtgtttgt catagcgacc ttcactttgc ccgcaatgat tggggaatga
     3121 ccgtgtatcc gattgtcccc ggtcatgaga ttgtgggaaa ggtaacagca gtgggtagcc
     3181 atgtaaagaa ttttaaagta ggagacctgg ccggtatcgg ttgcctcgta gatagttgcc
     3241 gggaatgtga taactgtaaa gaaggactgg aacagtattg caacaatggt tttgtgggta
     3301 cgtatggcgg tatggagaaa gacggcagtg gcgctaccca tggcggttat agtaaatcca
     3361 tcctggcaca tgaagatttt gtattgcata tctctgataa acttccgtta gaagctgtgg
     3421 cgccattact ttgcgccggt attactactt actccccatt acgccactgg aaagtgggca
     3481 aaggaacaag agtgggcata ctgggcctgg gtggtcttgg ccatatggcg gtaaagcttg
     3541 ctgctgcgat gggagcagaa gtaacaatgc ttagtcatac tgcctccaag gaagctgatg
     3601 caaaaagatt aggtgcccaa aagtttgttt tgaccagcag tgaagaacag gtaaaaggtg
     3661 tgaccgggta ttttgatttt attctcgata cggtgtcagc cgatcatgat tataattttt
     3721 atctgagcct gttgcgttcc agtggtacga tggtctgtgt aggtgtgcct tcggctccgg
     3781 cggtagtacc ggccatgaac ctgatctttg gcagaaaaag tattgcgggt tcattgatcg
     3841 gcggtattcc ggaaacacag gagatgctgg acttctgcgc tgaacatggt attgtgagtg
     3901 atgtggaaat aattgcgatg agagatatca atgaagctta tgaacgtatg ctgaaaagtg
     3961 atgtgaagta ccggtttgtg atcgatatgt ctacgctgta aatataatta ccttctgttc
     4021 tgaaagaacc tattgcataa aacccaatca ttgtcatatt gagcaaagcc gaaatatgac
     4081 aatgattggt aaacaaattt gtcgcatttc gataaactca atgtgacaaa tttttttatt
     4141 cctataccag gtgggataaa aaacctcccg gcatgcaagc cgggagggtc gttccaatga
     4201 taccatgtcc acgtccttat cagaatttga ttccgatacc accggtcagg ttcagtacgg
     4261 atattttttg tttaaatttt tctccctcat caaaccattc gctgctactt gcggtaaatt
     4321 caggtttcat atacaggtaa tcaagattgg tgaaaatgaa catgttttta cctgttccga
     4381 atcgcagatc caaaccgccc tgtaatgcgg gtgcaatact ccaatgtgct ttttccagta
     4441 gttcaccctc ataacttatt tttggtgatt tagtattgac cacaccaccc atcaacctga
     4501 ggtcagtcca gatatttttg gcgaggccaa atgtcagcat cggaccggct gtgatgccat
     4561 agaactgcca gttgtccata atatccagtg tcacctcttc gttcaaacgg ccttcgagaa
     4621 caatatccat cactacattg tgcatattgt atttgttgta aaaaacagat gcagcaatgc
     4681 cggcattggg atgaaactgg taaccgtaat tcaaatcaat ggtaaaccca gttttggcaa
     4741 acccagcctg ttcgttgtca aaatcattgc tggcaaaatc tcccacagga aaagaagggc
     4801 ccgcatggaa ggcaagcaca gatttcgtag ctgttgccgt tgtcttcgca cttccctggg
     4861 ccagtattgt cccgctaacc agcagaacaa tcgtcatcaa gagaagcttt ttcataactc
     4921 acatttttaa ttttgtcatt tctcacaagt gtcataagcg cctttctaaa gtagtttgta
     4981 aacgggttaa taacaatgat aactggattg cgccatcttg attgatatca ttgtttccag
     5041 gtatgtcaag gtattacctt tataacatat gcatcgaaga aaaaaagata acgatatcgt
     5101 taatagaaaa atcctggctc ttaaagccgc caatagttca aatattattt cgtattgata
     5161 agtcattgaa gttttatatc ctaccactcc tgcagctgct tatctccgga aaacaatttg
     5221 tatttttaat acatgtcgtt tcgtttatta tttctttatt tattgactgg cattgctgca
     5281 acagcacagc aacaatacag tactgcagat ttgaagaatg gaaaattcaa agacgacagc
     5341 agttatatat attccttacc gtttgaaaca ggcaagaaag tattcctggc acagggttac
     5401 gaaagtatgt tcagccacaa atcttcaaag gcgctcgact ttaaagttaa gaccggtacc
     5461 aaagtatgtg ccgcaagaag cggcatagta gtggccgcaa gaaaagactc tgaccgcagg
     5521 ggattgaagc aggaaaatct gtctgacggt aattatattt ttatccggca tgatgatgca
     5581 tctgttgcac agtactggca ttttcgtaaa gacggtgtac tggtgaaggt gggtgattct
     5641 gttactacgg gccagttaat aggttttagc ggaaataccg gttattcagc tttcccgcat
     5701 ctgcattttg aagtgagagg gtttgatgcc ggcggaaatg aaactgatct ccccacaaga
     5761 ttttatacgc aacggggtgt tatctacctg caacccggaa agttttaccg cacccgtcgc
     5821 tgatatcata atcattgtcc ggcccgttgt aattcgctac cttgatactt cttaaagcaa
     5881 tggttatgat cgtcgtttat attttagccg ccctgatcgt catcctgttg atagtagcag
     5941 cactgatgcc taagatatat aatgtagaaa aaaatattat catcaaatgt tctgttactg
     6001 atgtaatgaa ccgcatcagc gatatgaatt attacagcca gtggaatccc tggcaacaaa
     6061 gtgatcccac cactaaaagc accatcaccg gcactccggg cactacggga cataaatatg
     6121 cctgggaagg taagaaagta ggagtgggga gtttaaccct gctgagcatg aaccataaac
     6181 atattcattt tgaactggag tttctgaaac catggaaaag taaagccaaa gacaattggc
     6241 tgtttgaacc ctggggtgat cataacgaaa caaaagtaac ctggcaaaac agcggcaacc
     6301 tgccctggcc catagcaagg ctgatagggc ccatgctcaa taaaaacctg aatcagcagt
     6361 ttgaacaggg cttgaataat ctgaaaaaaa tgtgtgaagg ctgatctttg atcgctacaa
     6421 cgggctatcc catttctata gacaagaaaa aagccggttc aaatatgacc ggctttttgt
     6481 ttcagggcta tactaggctg cttttgcagg cagtttttcc aattcattca ttaattcata
     6541 gtactcattg ggagatacag atgcctgtaa cacggccacc attttttcga aggctggtgc
     6601 cggcaacatc attttcatac ctgtcagcat ggcaagattt tcttttcttg tttgcgccgg
     6661 taacacatgt tttccccaga caaggaatat ttcaaaagga atgcttttta atatctgcat
     6721 atgcaaaccc atcagttctt cttcgctgaa atatttccat agcaattcct gtgttgttgt
     6781 ttcctcgtga atgatatgct ccaggtattg gccgtgataa gaagaaaaag caaggtaaaa
     6841 ctgaaaccct tcttcatctg gttgggagcc atcaaagctg tttagccatg tttcaagttc
     6901 ctcctgtact ttttccagca gttcatgttc ttccgtattt tcttcagacg atccgggagc
     6961 ttttgtttct aagacagcca gtataattgt ttcttcgata tgggcatgtt cctttaataa
     7021 atcaaacatt tcgttgcctg tttgtttcag tacatgcagt tcactttcat tggcataatc
     7081 agttctgccg gcgagcaatg aaaactcagc cataagtttt cgcagtgctt tatgcggggt
     7141 gctgaatatt tttattcttt tcataaaaaa ggtttaatgg ttaatgtata ctgattcagt
     7201 tgatttaaat tcatctcctg gtcaccgggt agctaactgt ggttccggca caatattttt
     7261 tacaggtttg gtggattgca ggtaagtgaa aatggctgta atgtcttctt cactcagttg
     7321 tttataattt tcccatggca tcggtggcaa caggtctctg ttggtttcga ttcctttaga
     7381 tttacccctg gtaagcgccg tcttaaattg ctgcaggctc cagttaccga tacctgttgc
     7441 atcagaagta atattggctg cataagaggt tcccatcggg ctcaccatgg ctgtgccttc
     7501 tgtataaaat aaagcccagc cattttttaa agcttcatta ttggctggtg gtaatgacat
     7561 atcagaccgg tgacccgata aaagacgggt agtgtccagt gcaggaccca taggcgtcat
     7621 tactttggga gagtgacaat cattgcaacc catggtggtt acaagatagt tcccccgttt
     7681 aaccagggag tctttactga tcgttgtctg aactgattgt gaggagcctt gtttgcaggc
     7741 ggtaaaagca ccaatagctg cggccatcat taaaaccgct gataaatgaa acttcttcat
     7801 aaaaaggttt ttggttaatg acacaaaact attattgcag ctttacctgt acactcacat
     7861 cttcatgtgg taatgacaag cggcaaacaa agaggaagta aggagaggat gttaattttt
     7921 gttagcagaa atttgattta ctcaatctta attttatctg ctgcagcttt ggctttcttt
     7981 cgtaattcat tgatatcagc atttatatcc tcatacacca atgcaacagc cattcttctg
     8041 tatggccttg ttgctggctt accaaatatg cgaacatctg tttgtttttc tgctaaagct
     8101 tcttcaattc ctgttatgat aaattgctcc gcagatttat tggccagtac tactgcactg
     8161 gcaccggctc tttccaattt aatttcagga atgggtaatc cta
//
LOCUS       DAORTI010000140         8167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_9785_length_8167_cov_8.985823, whole genome shotgun sequence.
ACCESSION   DAORTI010000140 DAORTI010000000
VERSION     DAORTI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_8167_cov_8.985823"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /locus_tag="PLO70_13995"
     CDS             complement(<1..2600)
                     /locus_tag="PLO70_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQZ75629.1"
                     /translation="MIKIFTLLCGIVFTAFTAGAQLLTWTPDFPKDNDNISITVDASK
                     GNQGLFNYATVSDVYVHTGVITNLSTSSTDWRYVKFNQNFNQPNAQLQATSLGANRWK
                     FDITNIRAYYGVPAGETIQKISILFRNGAGTTVQRNADASDMYIPVYDNSIATRFTVP
                     LFQPTYTRIPETINKQVGDNINLTAIANLSSTMKLFLNGTEIQTASGVTTISANPTLT
                     TPGNQTIRVDAINGATTKSESFQFFVASAPTVAPLPPGVRDGINYEAGNTSVVLVLYA
                     PGKNRVSVIGEFPGSNWAEQTNYVMNKTPDGNYWWLRITGLTPGTEYAFQYLVDGTLK
                     VGDPYAEKILDPFNDSFISPTTYPGLKAYPTGLTSGNVSILQTNAPAYNWTINNFSRP
                     DKRNLVIYEMLPRDFIAAHDWKTLRDTLNYLQSMGVNAIEIMPFNEFDGNESWGYNPA
                     YFLAPDKYYGPKNSLKEFIDSCHRRGISVIMDIALNHTTGANPLAALYWNSSTNQPAA
                     NNPWLNVSATHPYNVFNDFNHESLATRYFTSRVVEHWLQEYKLDGFRFDLSKGFTQNV
                     QCGGSTSNEACISEYHADRVAIWKRYYDTCQLKSPGSYVILEHFANNTEEIELSNYGM
                     LLWGNSNYNFSEASMGWVANSNFEGYLYSARGWANPHLVSYMESHDEERMMYRNLQFG
                     NSSNTSHNVRDLNTALKRVELCAGFFLSAPGPKMIWQFGELGYDFSINRCVDGTISND
                     CRLSNKPIRWDYLTVVQRKRLYDIFASVNKLRFHPWYKDVFIANNINIARSLNGAFKS
                     MTIRSATDSSMLCVVGNFDVTAQTAVFAFPAGGTWYDYLNGSTFTATGTAQNLTLQPG
                     EFHIYLNRNLT"
     gene            2785..4887
                     /gene="ligA"
                     /locus_tag="PLO70_14000"
     CDS             2785..4887
                     /gene="ligA"
                     /locus_tag="PLO70_14000"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQZ75630.1"
                     /translation="MYSKEQTKELQKLTAELIKKQSPFSIKEQDAEQLRDILRFHEYR
                     YYILNDPLISDYEYDQLYKALEKIEKENPGLITPDSPTQRVAKGLTRDFPTVQHLVPM
                     LSLNNSYNAEDLIDFDRKARELTGLDKIEYCVEPKFDGGSISLIYENDLLTRGATRGD
                     GVEGDEVTTNMKQIRSLPLSAKFSHYGLQQVEIRGEVLINKSNFKKYNEQLIEQGEDP
                     KANPRNAAAGTLRIKDSSEVAKRNLEAFVYHVSYFSLVKGKKTPEELTTHSGSLQLLW
                     DLGFRSPEKEKKLFKGIDPVIKYCRDFEIERDNLPYEIDGMVIKVNEIALQDKMGMTS
                     HHPRWAIAYKFKARQATSKLLNVEFQVGRTGAVTPVAKLDPVAVGGVTVSSISIHNEE
                     YIKEKDLRIGDAVLIERAGDVIPQIVKSLADARTGHEMKIHFPKTCPACNSKLFKEVE
                     EAVWRCTNIECPAQVVERIIHFVSKDAMDIRGFGDANVRKFYELGLLKDIPGIYTLDF
                     TKISGMEGFGQKSIVNLQQAISNSKKQPLHRLIYALGIRFVGETTAKTLAQAIDYLPD
                     FKNFSLEELQNLEDVGPKVAGSIHHFFSNKENIKMLDELEKLGLQLKNEKKQLTTGGN
                     LSGQSFLFTGTLPTLKRSDAEAMVEENGGQILSGVSAKLNYLVVGEDAGSKLEKAKKI
                     NTVKIISEDEFLQMIGKK"
     gene            5000..5560
                     /locus_tag="PLO70_14005"
     CDS             5000..5560
                     /locus_tag="PLO70_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HQZ75631.1"
                     /translation="MIKKLAGETWKPLTFSGWKQLRNRYAVSSFGRIASYKKDVLQDG
                     KILNGSLTTGYRTLNLHRPDSKGTLYIHREMAKLFLKKPSVNHKYVIHRNHNKLDNSI
                     KNLSWATLEKMMAHQQHSPAKIAYKKKQANKTVGLKLTATQVKKIKTILNSQNRNITI
                     QQLAQKYNVSEMTMYRIKSGENWGRI"
     gene            5642..6310
                     /locus_tag="PLO70_14010"
     CDS             5642..6310
                     /locus_tag="PLO70_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HQZ75632.1"
                     /translation="MLSPSTIKFLKDLKKNNNKPWFDKNRKEYEKAKADFSQYIQQVI
                     DQLAKKDPSVQSLVAKDCMFRINRDVRFSKDKSPYKTNMGAFINRGGKKSLFAGYYFH
                     CEPGQSFTGGGLWMPMPPELKKVRQEIDYNFAAFRKIISSKKFKSVYTDLSRDPEYVL
                     ARLPKGYEADNPAAAYLKLKSFVAMTSIKDTDLIAKDLVKKTVASFEALQPLIEFINH
                     SLTE"
     gene            complement(6299..7342)
                     /locus_tag="PLO70_14015"
     CDS             complement(6299..7342)
                     /locus_tag="PLO70_14015"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQZ75633.1"
                     /translation="MFAKTGMGGLKMKVAVVGATGLVGSKMLQVLAERNFPVTELIPV
                     ASEKSVGKEVEFKGKKYKVVSMTDAIAAKPAVAIFSAGGGTSLEWAPKFAEAGITVID
                     NSSAWRMDPAKPLIVPEINADILTANDKIIANPNCSTIQMVVALNPLYKKYGIKRIVV
                     STYQSVTGTGVKAVEQLRGERVKEVKGESSDYPMAYKYPIDLNVIPQIDVFLDNGYTK
                     EEMKMVNETCKIMRDNSIRVTATAVRIPVMGGHSEAVNVEFVKDFDLDEVKALLQSSP
                     GVVVVDDTTRQQYPMPMDAHEKDDVFVGRLRRDESQPNTLNMWVVSDNLRKGAATNAV
                     QIAEYLHSKGLLG"
     gene            7588..>8167
                     /locus_tag="PLO70_14020"
     CDS             7588..>8167
                     /locus_tag="PLO70_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQZ75634.1"
                     /translation="MKITLSLFVVLLLTPVCLLSQNRYDIVIDEIMADPSPQVGLPSN
                     EWIELKNTTAAPVNLQNWRIGDAGGQSGPMPNFTLQPDSFVIVCTGSAVAAMSAFGTT
                     ISVTSFPSLDNDGDQLFLKAANGTTIHAVGYTSGWYQNELKKEGGWTLEMIDTKSPCA
                     GISNWKASVHVTGGTPGKKNSVDAINNDATAPQ"
BASE COUNT         2508 a         1717 c         1647 g         2295 t
ORIGIN      
        1 gtcaggtttc tgttcaggta aatatgaaat tcacctggct gcagggtcag attttgtgca
       61 gttcctgttg cggtgaaagt acttccattc aggtaatcat accaagtacc tcctgcggga
      121 aatgcaaaga ctgcagtttg tgccgttaca tcaaaattac ccaccacaca caacatagaa
      181 gagtccgtag cactgcggat ggtcattgat ttaaaagctc cattcaggct ccgggctatg
      241 ttaatattat tggcaataaa tacatcttta taccagggat gaaaacgtag tttatttaca
      301 ctggcaaaaa tatcatacag cctttttctt tgtactacag tcaggtaatc ccagcgaata
      361 ggtttattac ttaacctgca atcattgctg attgttccat caacacagcg gttaatggaa
      421 aaatcatatc ccagttcccc gaactgccaa atcattttag gaccaggcgc agacaagaag
      481 aacccggcac aaagttctac ccgtttcaat gctgtattca ggtcccgtac attatgtgaa
      541 gtatttgaac tatttccaaa ctggagattc ctgtacatca ttctttcttc atcatggctt
      601 tccatataac ttaccaggtg ggggttggcc cagccccggg ctgaataaag gtatccttca
      661 aagtttgagt tggccaccca tcccatcgaa gcttctgaga aattataatt ggaattgccc
      721 caaaggagca tgccgtagtt tgatagttcg atttcttctg tattatttgc aaaatgttca
      781 agtattacat aactgccagg tgattttaac tggcaggtat cataataacg tttccagatc
      841 gccaccctgt ctgcatgata ttctgatata caggcttcat ttgatgtact gccaccacat
      901 tgtacattct gcgtaaaacc tttagacaga tcaaaacgga agccatccag tttgtattcc
      961 tgcaaccagt gttccaccac ccggctggta aaatatcgtg tggccagact ctcgtggtta
     1021 aaatcgttga atacattgta gggatgtgta gcagaaacat tcaaccaggg attatttgca
     1081 gccggctgat tagtactgct gttccagtat aaagctgcca gtggattagc tcccgttgta
     1141 tggttcaatg ctatatccat aatcactgag atccctcttc tatgacagct gtcgataaat
     1201 tctttcaggg agtttttggg tccataatat ttatccggcg ccaggaaata agcagggtta
     1261 tagccccagc tttcattacc atcaaattca ttaaagggca tgatctcaat agcatttaca
     1321 cccatactct gcaaataatt caatgtatcc cgcaacgtct tccagtcatg cgctgcaata
     1381 aagtctctcg gcaacatttc atagataacc aggtttcttt tatctggcct tgaaaagttg
     1441 ttgatcgtcc aattatatgc aggtgcattt gtttgaagga tacttacatt cccggaagtc
     1501 aacccggttg gataagcctt taatcccggg taagtggtag gagaaataaa actatcatta
     1561 aacgggtcta aaatcttttc tgcataagga tcccccactt ttaatgttcc atccactaaa
     1621 tattgaaagg catactccgt tcccggggtt aatccggtga tacgaagcca ccagtaattc
     1681 ccatcaggag ttttattcat tacataattg gtctgctctg cccaattact tcccggaaat
     1741 tctccaatca cagatacacg gtttttaccg ggagcataga gtaccaatac aacagaagtg
     1801 ttacccgctt cataatttat cccatctctg acccccggcg gtaaaggagc tactgttggt
     1861 gcactggcaa caaaaaactg gaagctttcc gattttgtgg tagctccatt tatagcatcc
     1921 accctaatgg tttgattacc gggtgtagtt aaagttggat tggcagaaat ggtggtaaca
     1981 ccgctggctg tctgaatttc agttccattc agaaataatt tcatcgttga cgaaagattg
     2041 gcaatcgctg taagattgat attatctccc acctgcttat tgattgtttc gggtatcctg
     2101 gtataggtgg gttgaaataa tggaacagta aaccgggtgg cgatagaatt atcatagaca
     2161 gggatataca tatcactggc atcagcatta cgctgaacag tggtaccggc cccatttctg
     2221 aagaggatag aaattttctg gattgtttct ccggcaggca cgccataata ggccctgata
     2281 ttggtaatat cgaatttcca tctatttgcg cccaacgatg tagcctgaag ttgtgcatta
     2341 ggctggttaa aattctggtt gaattttaca tagcgccaat cagtggacga agtactcagg
     2401 tttgttatca ccccagtatg cacatacacg tctgatacag tggcgtaatt gaataacccc
     2461 tggttccctt tggaagcatc aacagtgatg gaaatattat cattgtcttt gggaaagtca
     2521 ggtgtccagg ttaataattg tgcccctgct gtaaaggcag taaaaacaat accgcaaagc
     2581 agtgtaaaga tttttatcat agcaagctcg tttattactt caaaatcggt aagaacaaga
     2641 gatttgcatc atcttgttta gtaatccaat ttcgaaggta ataaatatgt acttacgaca
     2701 cattaatttt tccttctttc acattatcac ttaaaatttt tatctgtgag gcgctaactt
     2761 taccgggcaa tacctctcgg tcatatgtat tcaaaagagc aaacgaagga attacaaaag
     2821 cttacagctg aattgattaa aaagcagtcg cctttttcca ttaaggagca ggacgctgag
     2881 caactgcgtg acatcctccg atttcatgaa tacaggtatt atatattaaa tgacccgctg
     2941 atctccgatt atgaatacga ccagttgtac aaggcattag agaagattga aaaagagaac
     3001 ccgggtttaa ttacacctga ctcccccacc caaagagtag caaaaggatt aaccagggat
     3061 tttccaaccg tgcaacacct ggttcctatg ctatcgttaa acaactctta caatgctgaa
     3121 gaccttattg attttgacag aaaagcaaga gaactaacag ggttggataa aattgaatat
     3181 tgtgtggaac caaaatttga cggtggaagt atttcgctga tctatgagaa tgacttgtta
     3241 acccggggcg ctacaagagg tgatggcgtg gagggtgatg aggtaactac caatatgaaa
     3301 cagattcgtt ccctccctct ctctgcaaaa ttttcgcact acggattgca acaggtagag
     3361 ataagaggtg aagtgctaat caacaaaagc aatttcaaaa agtataatga acaactaatt
     3421 gaacagggag aagaccctaa agcgaatccc cgcaatgctg cggctggaac attacgaatt
     3481 aaggattctt cggaagttgc aaaaagaaac ctggaagcat ttgtatacca tgtcagttat
     3541 ttctccctgg taaaaggtaa aaaaacaccg gaggaattaa caacccattc cggttcattg
     3601 caactgctgt gggacctggg attccgaagc cctgaaaaag aaaaaaagct ttttaagggt
     3661 attgacccgg ttataaaata ttgccgtgat tttgaaattg aaagagataa cctgccttat
     3721 gaaatagatg gcatggtaat aaaggtgaat gaaattgccc tgcaggacaa aatgggcatg
     3781 acatctcatc atccccgctg ggccattgcc tataaattca aggcaaggca ggcaaccagt
     3841 aagctgctga atgttgaatt ccaggtagga agaaccggtg ctgtaacccc tgtggcaaaa
     3901 ttggacccgg tagcagttgg tggagtcacc gtcagcagta tctccattca taatgaagag
     3961 tatatcaaag aaaaagacct gcggatcggc gatgctgtat tgatagaaag agccggcgat
     4021 gtaattccgc aaattgtgaa atcactggcc gatgcaagaa ccggacatga aatgaaaatt
     4081 catttcccga aaacctgtcc tgcctgtaac agcaaactct ttaaagaagt agaagaagca
     4141 gtttggcgct gtaccaatat tgaatgcccg gcgcaggtag tggaacggat cattcatttt
     4201 gtcagtaagg atgcgatgga catccgcggt tttggcgatg ccaatgtccg caagttttat
     4261 gaactgggtt tactaaaaga tattcccggt atctacacac ttgatttcac aaagatcagt
     4321 ggtatggagg gctttggcca gaagtcaata gtcaatttgc aacaggcaat aagtaattca
     4381 aaaaaacaac cccttcaccg gctgatttat gcattgggca tccgttttgt tggtgaaaca
     4441 acggctaaaa cattagccca ggcaatagac tatttacctg atttcaaaaa tttttcattg
     4501 gaagaattgc aaaatctcga agatgtagga cctaaagtgg ccggaagcat ccatcatttt
     4561 ttcagcaata aagaaaatat caaaatgctg gatgaactgg aaaagctggg tttacaactg
     4621 aaaaatgaaa aaaaacaatt aacaacgggt ggcaatttat caggccagag ttttttattc
     4681 accggaaccc tgcccaccct caaaagaagc gatgcagagg caatggtgga agaaaatggc
     4741 ggacagatac taagcggcgt cagcgccaaa cttaattacc tggtggtagg tgaagatgcc
     4801 gggagtaaac tggaaaaggc caaaaagatc aacacagtaa agatcatttc tgaagatgaa
     4861 ttcctccaaa tgattggtaa aaaataatat tgctgatact tcttaattat tttaacttag
     4921 tattatataa actgctctga taacctccac gtaccgccgt tattaactgc aacctgttcc
     4981 cggctaaaaa atagttatta tgattaaaaa attagcaggc gaaacctgga agccacttac
     5041 attttccgga tggaagcaac ttcgcaaccg ttatgcagta tccagctttg gacgtattgc
     5101 cagttataag aaagatgtat tgcaggatgg gaaaatcttg aatggttccc tgaccacagg
     5161 ttatcgtacg ttaaacctgc accggcctga tagcaaaggc actttgtata tccatcgtga
     5221 aatggcgaag ctatttttaa aaaaaccctc cgtcaatcat aagtatgtta ttcaccggaa
     5281 tcacaataaa cttgataatt ctataaagaa tctcagctgg gctacattgg aaaaaatgat
     5341 ggctcaccag caacatagtc ccgccaaaat tgcttataaa aaaaagcagg ctaataaaac
     5401 cgtggggctt aaactaaccg caacccaggt taaaaaaata aaaactattc tcaatagtca
     5461 gaaccggaac atcactattc agcaattagc ccaaaagtat aatgtcagtg aaatgacaat
     5521 gtaccggatc aaaagcgggg agaattgggg aaggatataa aaaatttata gttattagtt
     5581 catagtttat agttggacct ggtattgcct atctgtatta tctaagcaaa aaagcaaaag
     5641 aatgctaagc ccttccacaa tcaaattcct gaaagacctg aagaagaata acaataaacc
     5701 ctggttcgat aaaaaccgga aagaatatga aaaggccaaa gcagatttca gccagtatat
     5761 tcaacaggta atcgatcagc ttgccaaaaa agacccttct gtacaatcat tggtagccaa
     5821 agactgtatg tttcgaatca accgtgatgt tcgtttctcg aaagataaat ctccttataa
     5881 aacgaatatg ggggccttta tcaacagggg aggtaagaaa tctttgtttg caggatatta
     5941 tttccactgc gagcccggcc agagcttcac aggcggtggt ttatggatgc cgatgccccc
     6001 tgaattaaaa aaagtaaggc aggaaattga ttacaatttt gcagcgttca ggaaaattat
     6061 cagttcaaaa aaattcaaat ctgtttacac tgatctttcc cgtgacccgg aatatgtgtt
     6121 agcccgttta cccaaagggt atgaagccga taatcctgct gcagcatacc tgaagctaaa
     6181 aagttttgta gcaatgacat caataaagga tactgacctt atagcaaaag acctggttaa
     6241 aaaaacggtt gcaagctttg aagcattgca accgctgata gaattcatca atcattcttt
     6301 aaccgagtaa tccttttgaa tgtaaatatt ctgctatctg caccgcattg gtggcagctc
     6361 cttttcttag gttatccgat actacccaca tattcaatgt atttggctga ctctcgtctc
     6421 gtctcaatct tcccacaaac acatcatctt tttcatgtgc atccatcggc ataggatatt
     6481 gctgcctggt cgtatcatct accaccacca caccgggaga tgattgcagc aatgccttta
     6541 cttcatccag gtcaaaatct ttgacaaact ctacattcac tgcttcactg tgtccgccca
     6601 taaccggaat acgaacagca gtggcagtaa cacgaatcga attgtcccgc atgatcttgc
     6661 aggtttcatt taccatcttc atttcttctt tggtgtagcc attatctaga aacacatcaa
     6721 tctgcggaat cacattcaga tcaattggat atttatacgc catcgggtaa tcggaacttt
     6781 cgccttttac ctcttttact ctttcacctc ttaattgttc aactgctttc acccctgtac
     6841 ctgttacact ctggtatgtt gaaacaacaa ttcgcttaat cccgtacttt ttatacaatg
     6901 gatttaatgc caccaccatt tgaatggtag aacagttggg gttggcaatg atcttatcat
     6961 tcgccgtaag aatatctgca ttgatctcgg gaacgatcaa tggtttggca ggatccattc
     7021 tccaggcaga tgagttatca atcactgtaa taccggcttc ggcaaatttc ggcgcccatt
     7081 ccagtgaagt gcccccccct gcggaaaaaa tagctactgc aggtttcgca gcaatggcat
     7141 ccgtcatact cactacttta tactttttac ccttaaactc cacttcttta cctactgatt
     7201 tttcagaggc cacgggtatt aattccgtaa ccggaaaatt tctttctgct aatacctgta
     7261 acattttgga gcctaccaat cctgtggcac cgactactgc aactttcatt tttaatcccc
     7321 ccatccctgt ttttgcaaac aaagttttta attaataatt atggttaaaa aaataaggcc
     7381 ttccatatgg aaggcctgct gaacttttat gttgttacac acataacgct gcaaaccttc
     7441 cctcaggaat gtttcttact acgttttgtg tgtttcattt tattcatacc gggtgcaaaa
     7501 ataggcgggt agttatttac agccaaaaat tcgcagaata attttgatac tatgcttata
     7561 taaaaatgat tatttaactt gtataggatg aaaattacct tatccctgtt tgttgtattg
     7621 ttacttacgc ctgtttgttt gttgtcacaa aacaggtacg atattgtgat cgatgaaata
     7681 atggctgacc cttctccgca ggttggatta ccttccaatg aatggattga attaaagaac
     7741 acaactgctg cacctgtaaa cttacaaaac tggcgtattg gtgatgccgg cgggcagagt
     7801 ggtcctatgc ccaattttac attacaacct gatagctttg taattgtttg tacaggcagc
     7861 gctgtggcag caatgtcagc ctttggaaca actatatccg tcaccagttt tccttcattg
     7921 gataacgatg gtgaccagct attcctaaaa gctgcgaacg gaacaacaat acatgcggta
     7981 ggttatactt ccggctggta ccagaatgaa ttgaaaaaag aaggcggctg gacactggaa
     8041 atgattgaca ccaaaagtcc ctgtgccggt atcagcaact ggaaagcaag tgtgcatgtg
     8101 acaggcggaa caccgggaaa gaaaaattct gttgatgcca ttaacaatga tgcaacagcc
     8161 ccgcagc
//
LOCUS       DAORTI010000141         8162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_9790_length_8162_cov_8.504626, whole genome shotgun sequence.
ACCESSION   DAORTI010000141 DAORTI010000000
VERSION     DAORTI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9790_length_8162_cov_8.504626"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLO70_14025"
     CDS             <1..456
                     /locus_tag="PLO70_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQZ75635.1"
                     /translation="NTGIAESFRYIGGGDPSTYLSNNAGYSDVDVTAVSNSPVNPAGT
                     QPVGGLFTIISQKWFALNGVNTLEVWTDYRRLGYNEVVSASSVNDRFVYGAGVGYDPG
                     PPISVAPQNTSTKIPVRYLYPQAEYNYNSANVAAEGSIDRYTKIFWDIN"
     gene            483..1427
                     /locus_tag="PLO70_14030"
     CDS             483..1427
                     /locus_tag="PLO70_14030"
                     /inference="COORDINATES: protein motif:HMM:NF020111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HQZ75636.1"
                     /translation="MKKTSLASVLFVALLAMTFTGCLKDKGFDNYEYGINDPDTQPPG
                     VGFPLGAKAKNPFGLDVSGSTQVVNDMVYVNLNAGNPAPSDITITLEIKSAEIISAYN
                     TANGTNVQVLNPSLYSVPLTLTIPAGQRNVQVPLTVPSTLTLNPNLAYGVGLRITAVS
                     GNYVIADNLKNLFIEFTIKNKYDGRYVLRGYHNRTPYTFPYETDMHMVTAGPDAVFFY
                     WPEVGGVGHPIGVGPNNALSWYGSTVAPVVVFNLTTNLVTNVYGSDPAGPPITLFTGA
                     GSRQSKWDPATRDITVDWNYNGNPLRAFFDDLEYIGPR"
     gene            1541..2350
                     /locus_tag="PLO70_14035"
     CDS             1541..2350
                     /locus_tag="PLO70_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75637.1"
                     /translation="MHHIFCRISIALAAILSVDIAAAQQLQHPVALSANSGSLSGSPT
                     KFGGLAPDSRGWTDPALNHNKLIQQQNGGDGTYKLIGTYKVIGTQFLFGEHHKGDMFA
                     KEVKAYNVYLSYNTYNQEVEFYSTSNPDKPLVREPGTLDSFTIQENASLGIRIPLKFV
                     YGSTLGAKDQYYYMEIYRGIKFSLYKRYKSGLDYVSNNLAQPELRQFDLHYEYYYTDA
                     AGKGVKKIKPNAGNVIKEFKSIKDLSSAVNNESFTSNPEQGLINAFTSLNN"
     gene            2392..3129
                     /locus_tag="PLO70_14040"
     CDS             2392..3129
                     /locus_tag="PLO70_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75638.1"
                     /translation="MSKHVKFVIAGLVFCFSAISSFGQAGFTPTVGWVDPAMNHNRLI
                     REQTADGVYKLVGTFKVIGTSYLFGERQKGNLFSPEAKAYNIFLSYNTYNQEVEFYSA
                     SNPDKPLIKEPGSVDSFMINENQSLGILNPLKFVYGNHLGATDKSYYMELFAGKRYSL
                     FKRYKSDLGYVSTNYVQSELRQFDLLFDYFYTDTEGKGVKKLKPNVSSIIKEFKSVKD
                     ISSVLTNDAFAVNPDDALKKAFDFLNN"
     gene            3139..3453
                     /locus_tag="PLO70_14045"
     CDS             3139..3453
                     /locus_tag="PLO70_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQZ75639.1"
                     /translation="MNDQQNIPNQLNIEISEEVAEGSYANLAIITHSHAEFVIDFVNV
                     MPGTPKSKVKSRIILTPQHAKRFMKALTENIDKYESVNGLIKDLEEIQLPLNFGGPTA
                     QA"
     gene            complement(3566..4258)
                     /gene="radC"
                     /locus_tag="PLO70_14050"
     CDS             complement(3566..4258)
                     /gene="radC"
                     /locus_tag="PLO70_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQZ75640.1"
                     /translation="MQEHKYSIKQWSKDDRPREKLLYSGAENLSNSELLAILIHNGTK
                     QKSAVDVAKEVLKLGKDNLVELGKLSVKELMKIKGIGEAKAISIAAALELGRRRQAAA
                     SLEKTMVKTSSDIASYLQTKLKDFRHEVFAVLYLNRANKINHFEIISEGGITGTVADP
                     RIILRKALEEDAVNLILCHNHPSGSLKPSRADEQLTTKIKEAARFLDITVLDHIIVSD
                     DGYYSFADEGLI"
     gene            4367..4585
                     /locus_tag="PLO70_14055"
     CDS             4367..4585
                     /locus_tag="PLO70_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75641.1"
                     /translation="MKTRTFTPMGSLHPILFFVVVYVVALMLSIFICSSLFYTFNSSS
                     EKIGDVEKSQKQMPGSTVVAASTVALHR"
     gene            4667..5614
                     /locus_tag="PLO70_14060"
     CDS             4667..5614
                     /locus_tag="PLO70_14060"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQZ75642.1"
                     /translation="MQYAKIFSGTGSQKLAEQICKRYGTKLGKVNIQKFSDGEISPIF
                     LESVRGDYVFLVQSTFSPAENLMELLLMIDAARRASAYKVIAVIPYYGYARQDRKDRP
                     RVAIGSKLVANMLVAAGADRVITMDLHAPQIQGYFDIPVDHLDSHAVFIPYIENLKLE
                     NLTFAAPDVGATNRIREIASYFNAEMVICDKHRKRANEIASMVVIGDVTDRDVVIIDD
                     ICDTGGTLAKSAGLLKEKGARTVRALITHPVLSGKAYENIENSVLEELIVCDTIPVKK
                     ESQKIKVLSVAELFAVAIRNAYENKSITSLFIHSQRRDK"
     gene            5689..6315
                     /locus_tag="PLO70_14065"
     CDS             5689..6315
                     /locus_tag="PLO70_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQZ75643.1"
                     /translation="MKTITIEGQLRTGFGKGATRQLRSQELVPGVIYGGSQEVNFHAP
                     AAAFKKLVYTPSFQIAEVKLDGKTYRTIMKDLQFDKVDDKLIHVDLLELVEDKKVIAD
                     IPLKFVGVSKGVKDGGKLVTKMKSLKIKTYPKYLKAQIEVSIDNLELNGNIRVEDVKE
                     EHYEILNSPRIPIASVVLTRQLKQEEATAAPAAGAAPAAAAPAAAAKK"
     gene            6402..6977
                     /gene="pth"
                     /locus_tag="PLO70_14070"
     CDS             6402..6977
                     /gene="pth"
                     /locus_tag="PLO70_14070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQZ75644.1"
                     /translation="MKFLIVGLGNIGDEYAHTRHNIGFDVVNAFVQKHGGSFRVDRLA
                     YVADIKWKRKTFICVCPTTYMNLSGKAVKYWMDKEKVLLENTLVVVDDLALPLSKIRV
                     KPGGSDAGHNGLKSLQEVLGTRDYPKLRFGIGSDYPKGHQSDFVLGKWKKEEEPLVKL
                     KIQQAVEVIEVFAAQGIATAMNQVNNKEFSL"
     gene            7150..7761
                     /locus_tag="PLO70_14075"
     CDS             7150..7761
                     /locus_tag="PLO70_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQZ75645.1"
                     /translation="MKIFCVGRNYVAHAQELGNEVPDEPVIFMKPKSALLQPHTPFYY
                     PEFTNELHYECELVLRICKNGKYIQEKFASKYYDAITAGIDFTARDIQNELKEKGLPW
                     EKAKAWDNSAVIGKWVPFANIKNKKDINFCLYKNKELVQQGNSVNMIHSFDQIVSYIS
                     NYFSVNIGDVIFTGTPAGVGEVVVGDELEGFIEDDSMFTLEVK"
     gene            7902..>8162
                     /locus_tag="PLO70_14080"
     CDS             7902..>8162
                     /locus_tag="PLO70_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQZ75646.1"
                     /translation="MIDTNILLRAYRFMSQVKAMAETYEANRSVCKYVHSTSRGHEAI
                     QLAAAFQLQPQDFVSPYYRDESMILGLGFTPYHQMLQLLAKRG"
BASE COUNT         2450 a         1557 c         1780 g         2375 t
ORIGIN      
        1 aatacaggta ttgctgaaag tttccgctac attggtggtg gtgatccttc cacttacctg
       61 tcaaacaatg ccggttactc tgatgtggat gttacagcgg tatcaaactc tccggtaaat
      121 cctgccggca cgcaacctgt gggtggtctc tttacgatta tatcacagaa gtggtttgca
      181 ctgaatggtg taaatacact ggaagtgtgg acagattatc gcaggcttgg ttataacgaa
      241 gtggtttctg cttcttctgt taatgacagg tttgtttatg gagctggtgt tggttatgat
      301 ccgggtcctc ctatttctgt ggcacctcag aatacatcaa ccaagatacc agttagatac
      361 ctttacccgc aggcagaata caattacaat tctgcaaatg tggctgctga aggaagtatt
      421 gacaggtaca ccaaaatttt ctgggatatc aattaatttt gcacacctct aaattgaaaa
      481 atatgaaaaa aacaagttta gcttcagtcc tttttgtggc cctcctggca atgactttta
      541 cgggctgttt aaaagacaag ggctttgata actacgagta cgggatcaat gatccggata
      601 cacaacctcc tggtgttggt ttccctttgg gagccaaagc aaagaatcct tttggattgg
      661 atgtttccgg cagcacccag gtagttaatg atatggtata tgtgaacctg aatgcaggta
      721 atcctgctcc ttctgatatt actattacac tggaaattaa aagtgctgag atcatcagtg
      781 cttataatac agccaatggt accaatgtac aggtgttgaa tccttccttg tatagtgtac
      841 ctttaacact tactattccg gctggtcaac gtaatgtaca ggttccttta acagtaccca
      901 gtacacttac gctgaatcca aaccttgcat atggtgttgg tttgagaatt acagctgttt
      961 caggtaatta tgtaattgct gacaacctga aaaatttgtt catcgaattc actattaaga
     1021 ataagtatga tggcaggtat gtactgagag gttatcataa ccgtacacca tatactttcc
     1081 cgtatgaaac agatatgcat atggtaacag ctggacctga tgcagtgttc ttttactggc
     1141 cagaggttgg tggagtgggt caccctattg gtgtaggacc taacaatgca ttaagctggt
     1201 acggatcaac agttgcacct gtggttgtat ttaatcttac aacaaacctc gttaccaatg
     1261 tatatggcag tgatccggct ggtcctccca ttaccttatt tactggtgca ggaagcaggc
     1321 aaagcaagtg ggatccggcc acaagagata ttacggtaga ctggaattac aatggaaatc
     1381 ctctccgtgc tttctttgat gacctggaat atataggtcc gagataataa atgcttttga
     1441 tattttaaaa gggattgttt aattaacaat cccttttttt atctggcggg gtcaactgta
     1501 agtttttagt acttttattg cctaattaaa ttttatagtt atgcatcaca ttttttgcag
     1561 gatcagcatt gctttagcag caatattgtc tgttgatata gctgctgccc agcaattaca
     1621 acatccggtt gcattatctg ccaattcagg ctcattatcc ggaagtccta ctaaatttgg
     1681 tggtttggct ccagacagca gaggctggac agatccggcg cttaaccaca acaaattaat
     1741 tcagcagcaa aacggcgggg atggcacata taaattaatc ggtacgtata aagtaatagg
     1801 tacccagttc ttgtttggag agcatcacaa gggtgacatg tttgccaaag aagtaaaggc
     1861 ttataatgtt tatctcagct ataacactta caatcaggaa gtggagtttt attccacttc
     1921 caacccggat aaaccattgg tgagggagcc gggaactctt gattcattta cgatacagga
     1981 gaatgcaagc cttggtatca gaataccact aaagtttgta tatggctcaa ccctgggagc
     2041 aaaagatcag tactattaca tggagatcta tcggggaata aaattcagtt tgtataaacg
     2101 ttacaaatcc ggactcgatt atgtctctaa taacctggcc cagccggagt tgcgccagtt
     2161 tgatctgcat tatgaatact attacaccga tgctgccggc aaaggcgtta aaaaaataaa
     2221 acccaatgca ggtaatgtga tcaaagaatt taaatcaata aaagatcttt cctcagcagt
     2281 aaataatgaa tcctttacgt ctaatcccga gcagggtttg ataaatgcct ttacctcgct
     2341 gaataattaa tgctggaaaa ctaagattta agaactttaa aatcaggaag aatgagtaaa
     2401 catgtaaaat ttgtaattgc cgggttggta ttttgttttt cggccatcag ttcatttggt
     2461 caagctggtt ttactccaac ggtcggctgg gtagatccgg ccatgaacca taacaggtta
     2521 ataagagaac aaaccgctga tggggtgtat aaactggttg gaacctttaa agtgattggt
     2581 acctcctatt tatttggcga aagacaaaaa ggcaacttgt tctctccgga agcaaaagct
     2641 tacaatattt ttctcagcta taacacttat aaccaggaag tggagtttta ttccgcctcc
     2701 aaccctgata agccattgat aaaagaaccg ggttctgttg attcttttat gatcaatgag
     2761 aatcagagcc tggggatttt gaacccactt aagtttgttt acggaaacca tcttggtgcc
     2821 actgataagt cgtattacat ggaattgttt gcaggtaaaa gatacagcct gtttaaacga
     2881 tacaaatcag atctcggtta tgtttccact aactatgtac aatcagaact caggcagttc
     2941 gatcttttat ttgattattt ttacaccgat acagaaggta agggggtaaa gaaattaaaa
     3001 ccgaatgtta gtagtattat taaggagttt aaaagtgtaa aagatatttc ctctgtatta
     3061 actaatgatg ctttcgccgt aaatcccgat gatgctttga aaaaagcatt tgatttcctc
     3121 aataattaat tattatacat gaatgatcag cagaatatac cgaaccagct taatattgaa
     3181 atatcagaag aagtagcaga aggttcttac gccaatctgg ctataatcac tcactctcat
     3241 gcagagtttg ttattgactt tgtgaatgta atgcccggta caccaaaaag caaagtaaaa
     3301 tcgaggatta tcctgactcc tcaacatgcc aaacgcttta tgaaagccct gacagagaat
     3361 attgataaat atgaatctgt aaatggcctt attaaagacc tggaagaaat acaattgcca
     3421 ttaaattttg gagggccaac ggcgcaggca taatatgctt ataatctgta gttcgccatt
     3481 tggggttaca agtaatcggg aaaatcattt ttgtccggct attaattctt ataaactttt
     3541 gaaaccctga acttgtaaca ctaaactaaa taagtccttc atcagcaaaa ctgtaatagc
     3601 catcatcact aacaataatg tggtctaaaa ccgtaatgtc gaggaaacgg gcagcttctt
     3661 ttattttggt agtcaattgt tcatctgccc ggctcggttt caggctgcct gaggggtgat
     3721 tgtggcagag tataaggttc acagcgtctt cttccagtgc ttttctcagg atgattcgtg
     3781 gatcggccac cgttccggta atgccacctt cgcttataat ctcaaaatgg ttgatcttat
     3841 tggccctgtt caggtatagc acagcaaaaa cctcatggcg gaaatcttta agttttgttt
     3901 gcaggtaact ggcaatatcg ctgctggtct ttaccattgt tttttcaagg gaggctgcag
     3961 cctgtcttct tcttcccaat tcgagtgcag cggcaatgct gatagctttg gcttcgccaa
     4021 ttccttttat tttcatcagc tctttaacag agagcttacc taattctaca aggttatctt
     4081 ttcccagttt cagtacctct tttgccacat caacagccga tttttgctta gtaccattgt
     4141 ggatcaggat cgccagtaat tcagaattgc tgaggttttc tgcacctgag tatagtaatt
     4201 tttctcttgg cctatcgtct tttgaccatt gcttaattga gtacttgtgc tcttgcatga
     4261 aacaagatta gccaataatt ttaatcctgt caataccatt tagcggtact gataaaaagc
     4321 tgttaaaaaa tccgacctat ttgaaaaact ttaaaatcta atgcgtatga aaaccaggac
     4381 ctttaccccc atgggatctc tgcatcctat ccttttcttt gttgttgttt atgtggtggc
     4441 attaatgcta tcaattttta tctgttcatc ccttttttat acgtttaatt cctcttctga
     4501 aaagataggt gacgtggaaa aaagccagaa gcaaatgccc ggaagtactg ttgttgcagc
     4561 cagtacagtc gccttgcatc ggtaatcctt tcctgggaca atattttttt gaccagcttg
     4621 cggtgattgc ggtgagagta atatcttcgc cgcatataat cccaccatgc aatacgctaa
     4681 aatcttctcc ggaacaggct cacagaagct ggccgaacaa atttgtaaac gatatggaac
     4741 caagctgggt aaagtcaaca ttcagaaatt cagtgatgga gaaataagcc caattttcct
     4801 ggaaagtgtg agaggagact atgttttcct tgttcaaagc actttttcac ctgcagaaaa
     4861 cctgatggaa ttgctgctga tgatcgatgc agcccgaagg gcatccgcct acaaggttat
     4921 cgctgtgata ccttattatg gttatgcccg gcaagaccgg aaagaccgcc cacgggtagc
     4981 catcggtagt aagctggtag ccaatatgtt agtagctgcc ggagctgaca gggtgatcac
     5041 tatggatttg catgcacccc agatccaggg ctattttgat atacccgtgg accaccttga
     5101 tagccatgct gtttttatcc cttacataga gaatttgaag ctggaaaacc ttacctttgc
     5161 ggcccctgat gtgggagcta ccaatcgtat cagggaaatt gccagttatt tcaatgccga
     5221 aatggtgatt tgtgataagc accgcaaaag ggccaatgag atagccagca tggtggttat
     5281 aggagatgtg actgacaggg acgtggtaat tattgatgat atctgtgata ctggcggtac
     5341 actggcaaaa agtgcaggtc ttttaaagga aaagggggca agaacagtaa gggctttaat
     5401 aacccacccg gtgttgagtg gaaaagccta cgaaaatatt gagaatagtg tgctggagga
     5461 gttgatagtg tgtgatacga taccggttaa aaaggagagt cagaaaataa aagtgctcag
     5521 tgtggctgaa ctttttgcgg tagctatcag gaatgcatat gaaaataaga gtataacaag
     5581 cctttttatt cattcgcagc gacgggacaa gtaaataatt aaacaagata aaatgcagcg
     5641 ccgcaaggca actgcatcag accattttaa atcaataaaa aataagaaat gaaaacaatt
     5701 acaatcgaag gacaactgag gaccggattc ggcaaaggcg ccacccgaca actccgctct
     5761 caggaactgg tgcctggtgt aatttatggc ggctctcagg aggtcaattt ccatgctcct
     5821 gctgcagctt tcaaaaaatt agtgtacaca ccaagtttcc agatcgcaga agttaaattg
     5881 gatggcaaaa cgtacaggac tatcatgaaa gacctgcagt ttgacaaagt ggatgataaa
     5941 ctgatccacg ttgatttgct ggaactggta gaggataaga aagtgatcgc tgatattccg
     6001 ttgaaatttg ttggtgtttc aaaaggtgta aaagatggcg gtaagctggt taccaagatg
     6061 aagtctctga agataaagac ctatcccaaa tacctgaaag cacaaattga agtatccatc
     6121 gataatctgg aattgaatgg aaatatcagg gttgaggatg taaaggaaga gcattatgag
     6181 atacttaact ccccccgtat tcccatcgct tcagttgtgt tgacaagaca attgaaacaa
     6241 gaggaagcaa ctgctgcccc ggctgcaggt gctgcacctg ctgctgctgc tccggccgct
     6301 gctgccaaaa aataagtttt ttaaaactaa tcatagaccc gtatcaattg atgcgggttt
     6361 tttattgtct atacccgata tttgacttaa gtaaattgtg tatgaaattt cttattgttg
     6421 gcctcggaaa tattggtgac gaatatgcgc atacaaggca taatatcggt tttgatgttg
     6481 tgaatgcgtt tgtacaaaag catgggggaa gcttccgggt tgaccgatta gcctatgttg
     6541 cagatattaa atggaaaagg aagacattta tttgtgtttg tcccacaacc tatatgaacc
     6601 tgagcgggaa agctgtgaaa tactggatgg ataaagagaa agtgttgctg gagaatacat
     6661 tggtagttgt tgatgacctg gcacttccat taagcaaaat ccgggtaaaa cctggcggga
     6721 gtgatgccgg acataatgga ctgaaaagcc tgcaggaagt cctgggcacc agggattatc
     6781 ccaaactgcg gtttggaatt ggcagtgatt acccaaaagg tcaccagtca gattttgtat
     6841 tgggaaaatg gaaaaaggaa gaagaaccat tggtgaaact aaaaatacaa caggcagtcg
     6901 aagtaattga agtctttgct gcacagggta ttgccactgc catgaaccag gtaaataata
     6961 aagaattttc gctatgagaa atcagatgga ttacttactt tagcatccct gaccctcaat
     7021 cccggaggtc tctgattaaa ataaataccg ggaaaatcca ataaacatat tgattaccac
     7081 tgaatatatg ggataacagc cctatgagtt ttgtatccga tgtatttaat taaaatatga
     7141 cttcctgtta tgaaaatttt ctgtgttgga cgtaactatg tagcgcatgc ccaagaactt
     7201 ggtaatgaag tgcccgatga accggtcata tttatgaagc caaaaagtgc cttgctgcag
     7261 ccgcataccc cgttttatta tcccgaattc accaacgagt tacattacga atgtgaactt
     7321 gttttgcgta tctgcaaaaa cggtaagtat atccaggaaa aatttgccag taaatattat
     7381 gatgcgatta cagccggtat tgactttacg gcccgtgata ttcagaatga actgaaagaa
     7441 aaaggattgc cctgggaaaa ggctaaggcc tgggacaatt cagcggtgat cggtaaatgg
     7501 gttccttttg ccaatatcaa gaataaaaaa gatattaatt tctgcctcta caagaacaaa
     7561 gaactggtac aacagggaaa ttcagtgaac atgatacata gctttgacca gatcgtatct
     7621 tatatttcaa attacttttc agtgaatatt ggtgatgtga tttttacagg tacaccggcg
     7681 ggagtgggtg aagtagttgt gggagacgaa ttggaaggat tcattgaaga cgacagcatg
     7741 tttacgctgg aagtaaaata agaattgacg gacaaaaatt tttcctatac tgtaagccaa
     7801 gaccaactgc aacaggaagg ccattcgttt aaaagcgggt ggcttttctt ttacggggca
     7861 gggctttccc ttatttttgc tccctgcaat gattgctcta catgattgat accaatatat
     7921 tactacgggc ctatcgcttc atgagccagg taaaagcgat ggctgaaaca tatgaagcta
     7981 accgctctgt ttgtaaatat gttcattcta cttcaagagg gcatgaggct atacaactgg
     8041 ctgctgcttt tcaattgcaa ccacaggatt tcgtaagtcc gtattacagg gatgaaagca
     8101 tgatacttgg attaggattt actccatacc atcagatgct gcaattatta gccaaacggg
     8161 gt
//
LOCUS       DAORTI010000142         8082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_9929_length_8082_cov_6.065155, whole genome shotgun sequence.
ACCESSION   DAORTI010000142 DAORTI010000000
VERSION     DAORTI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_8082_cov_6.065155"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..246)
                     /locus_tag="PLO70_14085"
     CDS             complement(43..246)
                     /locus_tag="PLO70_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HQZ75647.1"
                     /translation="MAITIITVVAVLGSCTSVKEYQKNKLNDAEMVLGSRKIQKTESS
                     FQTYREGASGANGGKTGGGCGCN"
     gene            complement(279..1175)
                     /locus_tag="PLO70_14090"
     CDS             complement(279..1175)
                     /locus_tag="PLO70_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQZ75648.1"
                     /translation="MMGNRFEITVVSENENEALARIDEAIVEIRRIEKLLTTFDENSQ
                     TNLINRYAGVKPVKVDREMVDIIQRSKKISDITQGAFDITYGSVDKKLWNFDKNMTSL
                     PDADLARKMVRLINYKNVIVEEKKSTVFLKEKGMRIGFGGIGKGYAAERAKFILQQRG
                     MKSGIVNAAGDLTVWGYQPNGKEWTIGIADPDAARHPFSYLSITDMAVATSGNYEKYV
                     MIGGKKYSHTIDPKTGLPVNGIKSVTIISPNAEIADAMATPVMIMGIKVGLDMINQIK
                     GVSCIIIDDSNKIYTSKNITLK"
     assembly_gap    1184..1283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1362..2684)
                     /locus_tag="PLO70_14095"
     CDS             complement(1362..2684)
                     /locus_tag="PLO70_14095"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQZ75649.1"
                     /translation="MKVMKFGGTSVGKPERMHHIAGLVTKETEPVIVVLSALSGTTNA
                     LVEIGEHISKGDRGGAKQCIDKLEAHYQAFITELVKKEESLAKAKAIIAEHFEFLNII
                     LKISFSEALSKDILAQGELLSTKLFCVYLEEQGISHLLLPALEFMTIDSYDEPQIGSI
                     KVKLTQLVQQHKDKKIFVTQGYICRNARGEVDNLKRGGSDYSASLIAAAINASVCEIW
                     TDIDGMHNNDPRVVKKTVPVEQMSFDEAAELAYFGAKILHPASIWPAQTYKIPVRLLN
                     TMQPEAKGTLITEEAGSVGVKAVAAKDNIIAIRIKSSRMLLAYGFLRKIFEVFEKYRT
                     PIDMITTSEVAVSLTIDNDTNLKSILKELEPFGTVELDKNQTIISVVGNEIAQTEDMV
                     KKLFGSIMNIPVRMVSYGGSPHNISLLIAAEYKTQILQQLNKGMFGLE"
     gene            complement(2778..3860)
                     /locus_tag="PLO70_14100"
     CDS             complement(2778..3860)
                     /locus_tag="PLO70_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HQZ75650.1"
                     /translation="MPDQLFSYQQLFDFTNSVFQKIGCSPEHAHTATTTLLAADLRGI
                     DSHGIARLSGYVRLWEVKRVNATPDIKIIHETPSTAVVDGDSGLGLVVAPFAMQIAIE
                     KAKKVGTGWVSVQNSNHFGIAGYHAMMALQYDMLGIAMTNASPLVAPTFSIDKMLGTN
                     PICVAAPAGKEPAFVADLATTTAANGKLEILQRKNLETPTGWVQDKEGNESHDPHILK
                     KGGALLPLGGDREHGSHKGYALGAIVDIFSALLSGANYAPWVPPFPAYVPMPAQQPGK
                     GIGHFLGAMRIDAFRPADEFKKDMDHWIQGFRSCKTIPGEEKVLVPGDPEREFEAIRS
                     RDGIPLLTPVVDDLQSLAGKFGLPVL"
     gene            complement(3957..5351)
                     /locus_tag="PLO70_14105"
     CDS             complement(3957..5351)
                     /locus_tag="PLO70_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ75651.1"
                     /translation="MSSRRKFLKQLGAASLIAPVYSYATISEEELEKRIIPYERKPSS
                     FDDINVGIIGFGIMGSRNAKTILQVPGIKLKAVCDLYKGRLERAKELYGTDLFVTQAY
                     QDVLNRSDIDAVIICTSDQWHDKISIDAMRKRKAVYCEKPVVHQLNQGWELINVQQQT
                     GVVLQVGSQRVSSIAYAEAKRHYKAGEIGQLNCIEASFDRHTALGAWQYTMPLDASMD
                     TIAWKKYLKADPNTPFDAKRFFWWRNYKEYGTGVAGDLFVHLLSGIHFLTDSKGPSRI
                     FATGDLSYWKDGRNVPDVMTGVMEYKATTEHPAFQVLLKVNLASGAEKVESGKVKFYG
                     TEGVIDFGWNDFTIYRNKFSSYPNIGGWDALDTYPEVMQKEIMKMYKQKYPEAARLQL
                     DASPIKFMAPDGYDDRLDHFINFFESVRNSKPVVEDAAFGFRAAAPCLACNESYFQKK
                     VINWDPVKMKLVSP"
     gene            complement(5388..6221)
                     /locus_tag="PLO70_14110"
     CDS             complement(5388..6221)
                     /locus_tag="PLO70_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQZ75652.1"
                     /translation="MKKYFIGVLFLLVHHYVFSQYGNKNNWKLSIQTWTFHKYSFLQS
                     VEKADSLGVKFLEVYPGQKVGGDIQGAFSYTLDKDSRDKLKQFLEYRGIKVVALGVID
                     KDYYNKDNLEKFFEFAKYMGISFITAEPEWQDLDEFNRLAGKYKVKVALHCHPKPSSH
                     YWHPDSTVAAMNGRQHIGAWPDIGHWARNGVNIPDAMKKVEGRIWGLHLKDIKEFNNT
                     AAEDTLLGQGVCDIPAVMKELKRQKFKGVVSIEYEVNPVKNMSEMQQNVLYYLGQLGK
                     L"
     gene            complement(6224..>8082)
                     /locus_tag="PLO70_14115"
     CDS             complement(6224..>8082)
                     /locus_tag="PLO70_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQZ75653.1"
                     /translation="STEVLVIGGGVGGTAAAIQSARSGAKTILVEPTNMLGGMLTAAG
                     VSCTDGNDQLPSGMWEELRQALYQHYGRKRLNTGWVSNTNFEPKVGDSIFKAWAVKEK
                     NLKVYFGYEFESVQKTGNKVTGATLRNKDNTAALKIKASVVIDGTELGDVFAQAGAAY
                     DLGMDDPAVSGEKEAREKNDIIQDLTWAAVLKDYGPGADKTIAKPEGYDAKKFYCTCT
                     DAPCNDKPWNGDKLKMLNYGKLPRSPGAKQDKYMLNWPPFGNDIYLNVVEEKPEVRSR
                     KYDSAKQHTLGFIYFMQTELGMKNIGLADDELDGGMALIPYNREGRRVRGLVRMNINH
                     IKNPYDHTLYRTGISVGDYPVDHHHARYPGKVPEIEFPPIPAYSIPMGALIPENIDGL
                     IVCEKGISVTNIVNGTTRLQPVVLLTGQAAGVLAAKAVQRHHKEKTGIRRVTVRSVQD
                     GLLKAKAYLLPFTDVKPGHPYWEQIQKIGVTGILRGTGKPEGWANKMFFYPDSTIIIR
                     ELEANIKDYLPAYEAVHAASLIGDSLLTISTAFHILERLHRIINRSYAKYPSVIGDIF
                     NTGDFNTIWNNIGLKEYQPNRLIKRYELAALLDSLLDPFSIRGKLITITGKL"
BASE COUNT         2089 a         1970 c         1420 g         2503 t
ORIGIN      
        1 cggataagca gatttttctc atataaatga ttttaaaaag gattaattac aaccacagcc
       61 accccctgtt ttaccaccat tagcaccgga agcaccttcc cggtacgttt gaaaactgct
      121 ttccgttttt tgaattttcc tgctgcccaa taccatttct gcatcattca acttattttt
      181 ctggtactct tttacagaag tgcaggatcc taataccgcc actactgtaa taatggtaat
      241 tgccattaaa gtaactggtt gattcctttt tgctttagtc atttgagggt tatatttttt
      301 gatgtataaa ttttattact atcatcgatg ataatacagc ttactccttt tatctggttt
      361 atcatatcaa gtcctacttt aatccccata atcataacag gtgtagccat agcatctgca
      421 atctcagcat taggactgat tattgttaca ctctttatac catttaccgg aagcccagtt
      481 ttcggatcaa tggtatggga atattttttt cccccaatca tcacatactt ttcataattc
      541 cccgaagtag caactgccat atctgtaata cttaaataag aaaatggatg acgggctgca
      601 tccggatcag caattccaat tgtccattct ttaccatttg gctggtatcc ccaaacggta
      661 aggtcacctg cggcatttac aataccactt ttcattcctc gttgttgcag aataaatttt
      721 gccctttcag cagcataccc tttaccaata ccgccaaatc caattcgcat acccttctcc
      781 tttaaaaaga cagtagattt tttctcctcc actattacat tcttgtaatt aataagccga
      841 accattttcc tggccaggtc tgcgtcgggt aatgaagtca tattcttatc aaaattccac
      901 aacttcttat cgacagatcc gtaagtaata tcaaatgctc cctgggtaat atcagagatc
      961 ttttttgacc gctgaataat atcaaccatt tctctatcca cctttacagg ttttacacca
     1021 gcgtaccggt taataagatt tgtctgactg ttttcatcaa aagtggtcaa taatttttca
     1081 atcctcctga tctcaacaat ggcttcatca atcctggcca aagcttcatt ttcattttca
     1141 gacaccacag tgatctcaaa ccggttaccc atcagtttaa gaannnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnttagtca gtcaggaaaa aagtgaaata agtcacttta
     1321 acagacttat accatcccaa tccgggcaac ttatgtggat gttactctaa tccaaacatg
     1381 cctttgttca gctgttgcaa tatttgtgtt ttgtattcag cggctatcaa taaggaaata
     1441 ttatgagggc tgccgccata gctgaccata cgaacaggaa tgttcatgat agaaccaaac
     1501 agtttcttca ccatatcctc agtttgtgca atctcattac caaccactga aataatagtt
     1561 tggttcttat ctaattccac cgttccaaaa ggctccagct cttttagtat tgacttcagg
     1621 tttgtgtcat tgtcaattgt caatgataca gccacttctg atgtagtgat catatcaatt
     1681 ggagtccggt atttctcaaa tacttcaaat atctttctta aaaaaccata agccagtaac
     1741 atgcggctgc ttttgatacg gatagcaata atattatcct tcgcggctac tgcctttact
     1801 cccacactac ccgcttcttc tgtgatcaaa gtaccttttg cttcaggctg catggtattc
     1861 agcaatctta cgggaatttt ataggtctgt gcaggccaga tagaagccgg atgcaggatc
     1921 ttcgccccga aataagccag ctctgctgcc tcgtcaaaac tcatctgttc cacaggtacg
     1981 gttttcttta cgaccctcgg atcattattg tgcataccgt ctatatctgt ccatatctca
     2041 caaacagagg cattgatagc agcggcgatc aatgatgcag aataatcact gcctccccgt
     2101 ttcaggttat ccacctcacc tcttgcattg cggcagatat agccttgtgt aacaaatatc
     2161 tttttgtctt tatgctgctg tactaattga gtcagtttta ctttaatact tcctatctgt
     2221 ggttcatcat aactatcaat cgtcataaat tccagtgcag gcagcaacaa atggctgatg
     2281 ccctgctctt cgaggtatac acagaaaagt ttagtggaca ataattcacc ctgtgctaaa
     2341 atatctttac tcaacgcttc actgaaagat attttcagga taatattcag gaattcaaaa
     2401 tgctctgcaa tgatcgcttt agcttttgcc agtgactctt ctttcttcac cagttctgta
     2461 atgaaagcct ggtaatgcgc ttccagttta tcgatgcatt gttttgcacc accccgatcg
     2521 ccttttgaaa tatgctcacc aatttctacc aatgcattgg tagttcctga taaagctgac
     2581 agtactacaa taaccggctc tgtctccttt gttaccagtc ctgctatgtg atgcattctt
     2641 tccggtttgc ccacactggt acctccaaac ttcattactt tcatgtttgt cccccctccc
     2701 gagattttaa ttgattaatt aacagcctta gcaagcaagg ccgcaaagat aagattcggc
     2761 agcaaagtat tattagatta aaggacaggc agtccaaatt ttcccgccaa cgattgcagg
     2821 tcatccacaa caggcgttag caatggaata ccatccctgc ttcttattgc ctcaaactct
     2881 ctttccgggt caccgggtac caaaaccttc tcttcaccag gaattgtttt gcaggaacga
     2941 aaaccctgta tccaatggtc catatctttt ttaaactcat ctgccgggcg aaaagcatca
     3001 atccgcatgg cccctaaaaa atgcccgatc ccttttccgg gttgctgtgc tggcattggt
     3061 acatacgccg ggaatggcgg tacccagggc gcatagttgg cgccactgag taatgctgaa
     3121 aaaatatcaa caattgcacc aagtgcatac cctttatggg aaccatgttc cctgtcgcct
     3181 cccaatggta ataaggcacc gccttttttt aaaatatgcg gatcatgtga ttcattgccc
     3241 tctttatcct gtacccagcc ggttggagtt tccagatttt ttcgttgcaa tatttccagc
     3301 ttaccgtttg ctgcagtggt agtagccagg tcggcaacaa aagctggttc cttaccggca
     3361 ggtgctgcca cacaaatagg gtttgtaccc agcatcttgt caatagaaaa agtaggcgcc
     3421 actaagggac tggcattggt catggcaatt cctagcatat catattgtaa agccatcata
     3481 gcatgataac cggcaatgcc aaaatggttg ctattctgca cacttaccca accggtgccc
     3541 actttctttg ctttctctat agcgatctgc atggcaaacg gagctactac taaacccaat
     3601 ccgctgtcgc catccactac tgcagtagaa ggtgtttcat gaataatttt tatatcggga
     3661 gtagcattga ctctttttac ttcccacaat cttacataac cactcaaccg ggctataccg
     3721 tgcgaatcaa ttccccgaag atcggcggct aataaagttg ttgttgcagt atgagcatgt
     3781 tctggtgaac aaccgatctt ttgaaaaact gaattggtaa aatcgaaaag ttgctggtag
     3841 gaaaatagtt gatctggcat gtggcaaaga taggttgtca ggctgagctt gtcgaagcct
     3901 gacaggctat ctatctatct atcaatcacc cttcgatact gcgcctgact ccttactcag
     3961 ggtgacacta acttcatctt aaccggatcc cagttaatta ctttcttctg aaaataactt
     4021 tcattgcaag ccaaacaggg agctgcggca cggaaaccga atgcggcatc ttctacaacg
     4081 ggtttgctgt ttcttaccga ttcaaagaaa ttgataaaat gatccaaccg gtcatcatac
     4141 ccatcaggag ccataaactt aatgggagaa gcatccagct gcaacctggc agcttccgga
     4201 tacttttgtt tatacatctt catgatctcc ttttgcatga cctcaggata agtatctaat
     4261 gcatcccaac caccaatatt cggatacgat gagaatttat tccggtaaat agtaaaatca
     4321 ttccagccaa aatcaatgac accttccgta ccataaaatt ttactttgcc actttcaacc
     4381 ttttctgcac cgctggcaag atttactttc agcaatacct ggaaagccgg atgttccgta
     4441 gtggctttgt attccattac tcccgtcatc acatcgggta cattgcggcc atctttccag
     4501 taactcagat caccggtggc aaatattctc gacggacctt ttgaatcggt gaggaaatga
     4561 atgccggata ataaatgaac aaacaggtcg cctgccaccc cggttccgta ttctttatag
     4621 ttgcgccacc agaaaaatcg tttggcatca aacggagtat tgggatcagc tttcaaatat
     4681 tttttccagg caatagtatc cattgaggca tccaatggca tagtatattg ccaggcacct
     4741 aatgctgtat gcctgtcaaa agaggcttca atacaattca actggccaat ttccccggct
     4801 ttataatgtc gttttgcttc tgcatacgca atactgctta cccgctgact gcctacctgc
     4861 aacaccactc ctgtttgctg ttgcacattg atcagttccc aaccctggtt gagctggtgc
     4921 accaccggtt tttcacaata caccgctttt cgttttcgca tggcatcaat ggaaatctta
     4981 tcgtgccact gatcactggt gcaaatgatg acggcatcaa tatcacttcg gttcaataca
     5041 tcctggtatg cctgtgtaac gaacagatca gttccatata actctttcgc tctttccagc
     5101 cggcctttat agagatcaca tacagctttt agtttaattc ccggtacctg gagtatggtt
     5161 ttagcattgc gactacccat gattccaaat ccaatgatcc cgacattgat atcatcaaac
     5221 gaagaaggct ttcgttcata cggaatgatt cgcttctcca gttcttcttc gctgatagtg
     5281 gcataagaat acaccggagc tatcaaggat gcggctccaa gctgctttaa aaattttctt
     5341 ctggatgaca tggcgcttat taatttttta ttgcagtact acccatttca aagtttaccc
     5401 agctgcccga gataatacaa cacattctgt tgcatttcac tcatgttctt aacaggattt
     5461 acttcatatt caatagagac cactcctttg aatttttgcc gtttgagttc cttcatcacg
     5521 gcaggaatat cgcatacacc ctgtcccagc aaagtatctt ccgccgctgt attattaaac
     5581 tctttgatat ccttcaggtg aagtccccag attcttccct ctactttttt catagcatcg
     5641 ggaatattga ctccgttcct tgcccaatga cctatatccg gccaggcacc tatatgttgc
     5701 ctgccgttca tcgctgctac cgtagaatcc ggatgccagt aatgactgga tggtttgggg
     5761 tgacaatgca aagccacttt cactttatac tttccggcca gcctgttgaa ttcatcaagg
     5821 tcctgccatt caggttcggc tgtgataaaa gatatgccca tatatttggc aaactcaaag
     5881 aatttttcca ggttatcctt gttgtaatag tctttatcaa tcactcccaa agccactact
     5941 ttaatacccc tgtattccaa aaactgtttt aatttatctc ttgaatcttt atcaagggta
     6001 taactaaagg caccttgtat atcaccaccc actttctgac cgggatacac ttccaggaat
     6061 ttcacgccaa ggctgtccgc tttctccaca gattgcaaaa aggaatactt atgaaaagtc
     6121 caggtctgta tggaaagttt ccagttgttt ttatttccgt attgggaaaa cacatagtga
     6181 tgtaccaata aaaacaaaac cccaataaaa tattttttca tcgttataat ttacctgtta
     6241 ttgtaattag tttgcccctg attgaaaaag gatcgagtaa agaatcaagc agtgcagcca
     6301 gttcatatct ttttatcagc cggttcggtt gatattcttt caacccgata ttattccaga
     6361 ttgtattaaa atcgccggta ttgaaaatat caccaataac agagggatat tttgcataac
     6421 tgcggttgat aattctatgg agccgttcca gtatgtggaa agcagtggaa atcgtcaata
     6481 atgaatcccc gatcaatgaa gctgcatgca ctgcttcata agccggcaga tagtccttga
     6541 tatttgcctc taattctctg atgataatag tgctatcggg gtaaaagaac atcttgtttg
     6601 cccaaccttc aggcttgccc gtacccctta agattccggt aacaccaatt ttttgtattt
     6661 gttcccagta agggtgacct ggttttacat ctgtaaatgg aagcaggtaa gcttttgcct
     6721 ttagcaatcc atcctgaaca gatcttactg taactcttct tatcccggtt ttttctttat
     6781 gatgtcgttg aacagccttt gccgccagca ctcctgccgc ctgcccggtc aataacacca
     6841 ccggctgtaa cctcgtcgtt ccatttacaa tattggtgac tgaaatgcct ttctcgcaaa
     6901 cgatcagccc atcaatgttt tccggtatca atgctcccat tggaatgctg taagcaggta
     6961 tgggcggaaa ttctatctcc ggcactttac cgggatatct tgcatggtga tgatctacgg
     7021 gatagtcacc cactgatatt cctgttctgt ataatgtatg atcatacgga tttttaatat
     7081 gattgatatt catccgcacc aatcctcgca ctcttcttcc ttcccggtta tacgggatca
     7141 aggccatgcc gccatccagc tcgtcatccg ccagtccgat atttttcatt cccagttctg
     7201 tttgcataaa gtaaataaag cccagcgtat gctgttttgc gctgtcatac ttcctacttc
     7261 tgacctccgg cttttcttcc actacattca aataaatatc attgccaaac ggtggccagt
     7321 ttagcatata cttatcctgt ttcgctccgg gacttctcgg caacttacca taattcagca
     7381 tttttagctt atccccattc caaggcttgt cattgcaagg tgcatcggta caggtacaat
     7441 aaaatttttt ggcgtcatac ccctctggct ttgcaatggt tttatcagca ccgggtccgt
     7501 aatcttttaa tactgccgcc caggtgaggt cctgtatgat atcatttttt tctcttgctt
     7561 ctttttcccc actcaccgca gggtcatcca taccaaggtc ataggcagcg ccggcttgtg
     7621 caaacacatc acccagttct gttccatcaa tcaccacact ggcttttatc ttcagagctg
     7681 ctgtattatc cttattacgc agtgtggccc cggttacttt gtttccggtt ttttgtaccg
     7741 attcaaattc gtaaccgaaa taaactttca gatttttttc tttcactgcc catgctttga
     7801 aaatactgtc gcccactttg ggttcaaagt tggtattgct tacccagcct gtgttcagtc
     7861 tttttcttcc ataatgctga tacaaagcct gcctcaattc ttcccacatg ccactgggta
     7921 actggtcatt tccatcggtg caacttactc ctgcagccgt cagcattccc cccagcatgt
     7981 ttgtaggttc taccaatatc gttttcgctc ctgatcttgc agattgaata gccgctgctg
     8041 ttccgcccac accacctccg atcaccaata cctccgtgct tg
//
LOCUS       DAORTI010000143         7993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_10088_length_7993_cov_7.181154, whole genome shotgun sequence.
ACCESSION   DAORTI010000143 DAORTI010000000
VERSION     DAORTI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10088_length_7993_cov_7.181154"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLO70_14120"
     CDS             complement(<1..1122)
                     /locus_tag="PLO70_14120"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ75654.1"
                     /translation="MYRSSCFSFIITAFFLLVLSLHSAGQPDKAEAAIRDIMQQSKVV
                     GLSVAVVKKGKLIYTHSFGLKDIDSNTPLTNDCLFRIASISKSFTATSIMQLAEAKKL
                     SLDDDISDLVGFSIRNPKFPNTVITLRMVLAHRSSINDSQGYFTLDAINPAKNPNWAN
                     CYNAYEPGTGYQYCNLNYNMAGTIIEKITGERFDQYVKHHILDRLGLYGGYCVDSLDA
                     SRFATIYEYDDSVKSFKASPNAYAPRREEIAKYVMGYSTPIFSPTGGMKISATDLARY
                     MTMHMNRGKANGKRIIKKNSAISMQTALSEKEGYGLAISTTAKLIAGKTLKGHTGSAY
                     GLYSAMFFHPEEKFGIVVISNGCDPRYTDGYNTVIRKTVN"
     gene            1270..1611
                     /locus_tag="PLO70_14125"
     CDS             1270..1611
                     /locus_tag="PLO70_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75655.1"
                     /translation="MLIVATELHVRNGWKFIPFVRHSIRSTKQAKQAAGCLHVWVGNN
                     GWRIGYTLTAWKDKSSMQQYRNTGPHKEAMQQIAKLSKQYKTLEWEAEQVPSWNEAKE
                     RLDAIAFKLLK"
     gene            complement(1698..2219)
                     /locus_tag="PLO70_14130"
     CDS             complement(1698..2219)
                     /locus_tag="PLO70_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75656.1"
                     /translation="MRYAALLTALLVLLQKTGAQQPAADKWKTVNAFSMGDNKSKYTD
                     QLLFWSKTGDGNNAYIYKTTGYKSQMNLEVFGWRYTSVYLIFNLQDELTGMQFITVYK
                     PNDYVADKDYYAQQEKLIASLGPYTKKENSYTDGVSSNGVTWSGNEVSISFVKKTGGK
                     NGGLANELIFLKK"
     gene            2784..3002
                     /locus_tag="PLO70_14135"
     CDS             2784..3002
                     /locus_tag="PLO70_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75657.1"
                     /translation="MHTNNVSLNGMYAVVLLLINAILLETALVKNTDWYKALWITLPL
                     LAIAIYQSKKKKIPAGKTPADTLLLLKK"
     gene            3006..3401
                     /locus_tag="PLO70_14140"
     CDS             3006..3401
                     /locus_tag="PLO70_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQZ75658.1"
                     /translation="MQITKQPVLLSRQDHSIILYHLRKEHVHNAADRQSANALQQELK
                     RARLMDENQLPPGMVRLGSRLKVRDIATNALLEIMIVTPDQANWKEKKISVLSPLGTA
                     LIGFCAGVSVNWKVPAGEKLFLIEEVNNE"
     gene            3610..4611
                     /locus_tag="PLO70_14145"
     CDS             3610..4611
                     /locus_tag="PLO70_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75659.1"
                     /translation="MGSKYILATFFLLAAINLKAQTPQFAVVRPNGTTYICPTFDSAY
                     NKAMDDDYIYLPGTVISGDKTITKKLTIIGAGHYPDSTQYTGKSLFTGQFLIEKKCTL
                     EGIEIANSLVIMNAAASNSSFIRIRASALYLGGTNDHFIDGSAISYITGRVYNSSSCV
                     SSANLFVKNSILQSVSSLQFSSFNNSIFLGSNSGWFYLKVANSSFINCIFRGLFNIDW
                     FAENNCYPLVGNSSNYCINPVAAGNNNYSSSQADTLFVNSPNASSFDYQYNYHLKPNS
                     PYLSAGSDGTEIGIYGGNSPYREGAVPSNPHIYFKQVSTQTNSNGQLPVQFKVRTSN"
     gene            4615..7377
                     /locus_tag="PLO70_14150"
     CDS             4615..7377
                     /locus_tag="PLO70_14150"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQZ75660.1"
                     /translation="MLRILNILLFLTLMLSLYQKGISQAKCGFDILHNKLLKNDSEYA
                     RTIEKNDERISKLLQSRQIKYYNRTLNESGVYVIPIVIHVMHTGGVEGTIYNPSKQQI
                     LSTIDYLNQVYNGSYPGIEGVGNIEIQFELAKRSPSSVCTDGIVRIDASSLPGYISNG
                     INADNINGCPELTLKNLARWNTNDYYNIWIVNKIDGEDGTSANPFIAGFAHFPGASSS
                     IDGTVLLATQMLPGLKTLPHELGHALNLYHTFQGSTSSNCPINLFCSLFGDRVCDTDP
                     NSQSFSCRTETNPCTSAPFSSNTEKNYMSYTSCFTLFTNGQKERMHASMSFESRETLV
                     RQGNLALIPPLPELTIISPILSQNIILAGGSLIASFAENNSGALAAQANYVNIHLSPD
                     PILTPGLNGDIYLDQFFVNQELSPLSQTILLNQSITIPNSTPPGTYFVFFAADGEGNV
                     AECDNDNNFATAQLIVSGSNTVSQFTYRYWFDNSFSQSIANNLTTGNTFNLQGLFATS
                     SLSPGLHYFHIHFKDSTNRWSSIVSSPIIKLNTAFPPGSSKYEYWFDNEYFLKETIYT
                     QNSINFLLLDTTNLDTLPNGLHTLHIRFKPEGKHWSSIVSSLFYKTGVSPFGNTEYEY
                     WLDNNYTGRSTISNTYTSNFILLDSIITIGVSNGLHTLHFRFRPDGKTWSSVTSSFFY
                     KDQNSITGINNLAQYVYWFDSNWQNPQTITITGVQNLNWTLNTSVDSLSNGPHVLSQA
                     FKDDKGQWSSIISTDFTKQPASTPSCLSSGRQFTAGIIAGGGTDYQWQLDNGTGFNDI
                     LNNAVYTGANTDTLELSNAPTSWYGYKFRCRVTNGATTFYGPMYTLKFVSTWTGTTDN
                     AWENPANWSCNTIPDANTDVYVNAGSVRYPEVNSTTATCRSLNLQPGTSFEVKPGFTI
                     NITGKN"
     gene            complement(7469..7669)
                     /locus_tag="PLO70_14155"
     CDS             complement(7469..7669)
                     /locus_tag="PLO70_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75661.1"
                     /translation="MKAKSMMTTRDAGRIQSAAGKNQFSKTFKTGFYQRAQSVAAKGN
                     GGLPSTTGRPSGGGRDNNPPKK"
     gene            complement(7704..>7993)
                     /locus_tag="PLO70_14160"
     CDS             complement(7704..>7993)
                     /locus_tag="PLO70_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75662.1"
                     /translation="KNLNDLKDLFDPLSHYLAMLSDSVIINVAFYQLSGKKELQPILP
                     FSEIKELSPFKFSASKAAKEYYRIFLKVQEAYQHRISFEKSILDWKKQHSH"
BASE COUNT         2417 a         1632 c         1595 g         2349 t
ORIGIN      
        1 attcactgtt ttccgtatca cggtattata accatcggta tacctgggat cacagccatt
       61 gctgatgaca acaataccga acttttcttc cgggtgaaaa aacatagcgc tgtacaagcc
      121 gtaagcagaa ccggtatgtc ctttcagggt tttaccggca atcagtttag cagtggtgga
      181 gatagccagt ccataccctt ctttctctga taaggctgtt tgcatactga tggcactatt
      241 cttttttata attcttttgc cattggcctt acccctgttc atgtgcatgg tcatatacct
      301 ggccaggtcc gtagcggaga tcttcatacc gccggtgggt gaaaaaatgg gagtactgta
      361 acccataaca tatttagcaa tctcttcgcg gcggggtgca tatgcattgg gtgaagcttt
      421 gaaacttttt actgaatcat cgtattcata aatggtcgca aagcgggaag cgtccaatga
      481 gtccacacaa tagccgccgt acaaaccgag ccgatcgagg atatgatgtt tgacatactg
      541 gtcaaaacgt tcgcctgtta ttttttcaat gatggtgccc gccatattat aattcaggtt
      601 gcagtactgg tagccggtac ccggttcgta tgcattataa cagttggccc agttggggtt
      661 tttagccggg ttgatcgcat cgagggtgaa ataaccctgg ctgtcattga tagaagaccg
      721 gtgtgctaat accatgcgca gggtgatcac ggtattggga aattttgggt ttcgtatgct
      781 gaagccgacc aggtcactga tatcatcgtc cagggacaat ttttttgctt cggccagttg
      841 cataatggaa gtggccgtaa aagatttgga gatagaagcg atgcggaaca ggcaatcatt
      901 tgttaatgga gtattgctat caatatcttt taacccgaag gaatgcgtat aaatgagctt
      961 gccttttttc accacggcta ccgaaagtcc caccactttg gattgctgca tgatgtcccg
     1021 gatggcggct tcggctttgt cgggttgacc ggcactgtgc agggacaaga ccagtaagaa
     1081 gaaagcggtg ataataaatg aaaaacagga ggaccggtac atggtaaaag tattagcggt
     1141 aaataatacg gaagctattt aaaagcgggg agataaacaa cgggaggggg attgttatta
     1201 cataaatggg ttactgatga gaatctaaat tttaaaatct ttgtattttt cctgtaccaa
     1261 ttattgtcta tgcttattgt agccaccgaa ttacatgtca ggaatggttg gaaattcatt
     1321 ccctttgtgc ggcattccat ccgttcaaca aagcaggcta agcaggcagc aggctgcctg
     1381 catgtatggg taggaaataa tggctggcgc atcggctata cgcttacggc ctggaaggat
     1441 aagtcttcca tgcagcagta ccgcaataca ggcccgcaca aagaagccat gcagcaaata
     1501 gctaaattat cgaagcagta taaaacgctg gaatgggaag cggagcaagt gcccagctgg
     1561 aatgaagcga aggaaaggct ggatgcaatt gcatttaagc tgttgaaata atcaaatgca
     1621 tgtggttggt aattatcttt tcacctacag tggcgtgaat agcagcaaca ggaataaagg
     1681 aacccgtact tatcttatta cttttttaaa aaaatcaatt cgttggcaag acccccgttc
     1741 tttccgccgg tttttttgac aaaagaaata ctcacttcat tacccgacca ggtaacacca
     1801 ttggatgaaa caccatctgt ataggagttc tccttttttg tgtatgggcc aagagaagca
     1861 attagttttt cctgctgggc ataataatcc ttatcggcaa cataatcatt gggtttgtat
     1921 actgtaatga attgcatacc ggtaagttca tcctgcaaat taaaaatcaa ataaacagac
     1981 gtgtatcgcc agccaaatac ctccaggttc atttgtgatt tatagccggt ggttttatag
     2041 atataagcat tattaccatc acctgtttta ctccaaaaca gcaactggtc agtatacttg
     2101 cttttattgt cgcccatgct gaaagcattg acagttttcc atttatcagc agcgggttgc
     2161 tgggcacccg ttttttgcaa cagtaccagc aaggccgtca gtaaagcagc atatcgcata
     2221 aaaatcattt tactaatgta ctcattttaa aagacgatcc aatatgtgta ggcgctagtt
     2281 tctctctctc tttttttgct cgtacaagaa acgggaatgc ataccgttta caccgtggtt
     2341 aatagagtta tgttggcaag ttcactaccg ttatctcaca accagtagtg tatgttaaaa
     2401 aatacatcaa tcaatctacc ggtcaataaa attgcttaat tttaaatttc atgaacaggt
     2461 tttcaaaata tacaacgctt ttgtttcccg gggcggtcat caccgacctc acggattctt
     2521 atacctgttc ctttacagag ttcatgcctt tctgtaatca tcttttttat ttttccagtt
     2581 tcaaagactt taagcagcta accccggcct cctgataaag accgggaagg atgtttgtct
     2641 gctgcggtat cgtatgcagg ttaatgctgt atgcatttag tattgcatac catttacaat
     2701 aacctgtagt atttaacgga gcagtgttaa ccggtttacc gtgcctggtg aacctattac
     2761 tttactttat tcaattaact atcatgcaca ctaataatgt ttctttaaac ggaatgtatg
     2821 cagtagtact gctgctgata aatgccatac tacttgaaac ggcacttgta aaaaatacag
     2881 actggtataa agcactctgg ataacactgc ctttattggc gatagctata tatcaatcca
     2941 aaaagaaaaa aataccggcg ggaaaaactc ctgctgacac tttattactt cttaaaaaat
     3001 aaaaaatgca aataacaaag caaccggtct tgcttagcag gcaagaccat tctattatat
     3061 tgtatcacct gcgcaaggag catgtgcaca atgctgcaga ccgtcaatca gccaatgcac
     3121 tgcaacagga gctaaaaaga gcccggctga tggatgaaaa tcagttgcct cccggaatgg
     3181 tgcggctggg cagccggtta aaagtgaggg acatagctac gaatgccctg ctggaaataa
     3241 tgattgtaac tcccgaccag gccaattgga aagaaaagaa aatatcggtg ctatctccgt
     3301 tgggtacagc cctcatcggt ttttgcgctg gtgtatccgt gaattggaaa gtgcctgccg
     3361 gggagaaatt atttttgatt gaagaagtga ataatgaatg agcggcagaa acttcataat
     3421 tgtgctgagt agttaactca gcacaattta cgccgttgtt ggtgttgtga atagacaaag
     3481 cagcaatgca agaacaccag caaaggctgt ttaaagattc ggaaagacca actgtagcga
     3541 aaaaacaaac tctcaattaa ggacatatta tggcgttttt gtatatttag aatactaaaa
     3601 ttaccggcta tgggaagtaa atatattctc gctacctttt ttctactggc agccattaat
     3661 ctgaaagcac agacaccaca gtttgctgtg gtgagaccaa atggcaccac ttatatatgc
     3721 ccgacttttg acagtgcata caataaagcc atggatgatg attatatcta tttgcccggc
     3781 acggtaataa gtggtgataa aaccattacc aaaaaactta cgattatcgg cgccggacat
     3841 taccctgact ccacacaata caccggaaaa tctcttttta ccggtcaatt cttaattgaa
     3901 aaaaaatgca cccttgaagg tatagagatt gccaatagtc ttgtgattat gaatgcagcc
     3961 gccagtaatt catcatttat ccgtataagg gcatcagcat tatatttagg tggtacaaat
     4021 gaccatttca ttgatggttc agccatttct tatattaccg gaagagtata taattcgtca
     4081 tcttgtgtat cctctgctaa tttatttgta aagaacagta tcctgcaatc agtttcctcg
     4141 ttacagtttt ccagtttcaa taatagcatt ttccttggat cgaattcggg ttggttttat
     4201 ttaaaagttg ctaacagtag ctttattaat tgtatttttc ggggactatt taatattgat
     4261 tggttcgctg aaaacaattg ttatccgctt gttggaaact caagtaacta ttgtataaat
     4321 cctgtagcgg caggtaataa caattactcc tcatcccaag ccgacacatt atttgtaaac
     4381 tcaccgaatg ccagctcttt cgactatcaa tataattatc atttaaaacc aaatagtcct
     4441 tatttatcgg ctggtagcga cggtacagaa attgggatat acggtggaaa tagtccttat
     4501 agggaaggcg ctgttccaag taacccacat atttatttca aacaagtttc aacacaaaca
     4561 aatagtaacg ggcaattacc tgtgcagttt aaagtgagga caagcaatta gtaaatgcta
     4621 agaattctaa atattctttt gttcctgact ttgatgttaa gcctatatca aaaagggatt
     4681 tcacaagcaa aatgcggctt cgacatttta cataacaaac tgctcaaaaa cgattcagaa
     4741 tatgcaagaa ctatcgaaaa aaacgatgaa agaataagca aattattgca gtctcgccag
     4801 ataaaatact ataatcgcac actaaacgaa tctggcgttt atgttatacc aattgttatt
     4861 catgtaatgc ataccggagg tgtagaaggg acaatttaca acccaagtaa acagcagatt
     4921 ctttcaacca tagattattt aaaccaggtt tataacggta gctatcccgg tatagaggga
     4981 gtcggaaata ttgaaataca gttcgaatta gcaaaaagaa gcccgagttc tgtatgtaca
     5041 gatggcattg taagaattga tgcatcttca ctaccgggtt atatttcaaa tggcatcaat
     5101 gctgataaca taaacggttg ccctgaatta acattaaaaa atttagcaag atggaataca
     5161 aatgactatt ataatatttg gattgtaaat aagattgatg gtgaagatgg tacgagtgca
     5221 aaccctttta ttgcgggttt tgctcatttt ccgggtgcct cttcaagtat agacgggaca
     5281 gttctgcttg caacgcaaat gctcccaggt ttaaaaactc taccacatga gttagggcat
     5341 gccttaaacc tatatcacac ttttcagggc tccaccagtt caaattgtcc aattaattta
     5401 ttttgctcac tttttggtga tagggtgtgt gacactgatc ctaattctca aagtttttcc
     5461 tgtagaacag agacaaatcc ttgcacaagc gctcccttca gtagcaatac tgaaaagaat
     5521 tacatgagct atacttcttg ctttacgtta tttacaaatg gtcaaaaaga aagaatgcat
     5581 gcgtcaatga gttttgaatc aagagaaaca ctagtaagac aaggcaactt agctctgatt
     5641 cccccgctgc ctgaattaac aataataagt cctatactat cacaaaatat aattttagca
     5701 ggtgggagtc ttatcgccag ttttgccgag aataattcag gagctttagc tgcacaagca
     5761 aattacgtca acattcatct ttcaccagat cctatactaa ctccaggttt aaacggtgat
     5821 atttatcttg atcaattttt tgtcaatcag gaactctctc cattatccca gacaatttta
     5881 ctaaaccaat ctattacaat tcctaattcg acaccgccag gaacttactt tgtctttttt
     5941 gctgcagacg gagaggggaa tgttgcggag tgtgacaatg ataataattt tgctacagct
     6001 caattaattg tatccggttc taatacggtt tcacaattca catatagata ctggtttgac
     6061 aatagctttt cgcaatccat agcaaataac ttaactacag gaaatacatt taatctgcaa
     6121 ggcttgtttg caacgtcttc cctatccccc gggctacatt acttccatat tcattttaag
     6181 gatagtacga acagatggag tagtattgta agcagtccga ttatcaaact taatacagcc
     6241 tttccaccag gctcatccaa atacgagtat tggtttgaca atgaatactt cttaaaagaa
     6301 acaatctaca ctcaaaattc tattaacttt ttacttttag acaccaccaa tttagacact
     6361 ctccccaatg gcctgcacac ccttcacatt cgttttaaac cagagggcaa gcactggagc
     6421 agtatagtgt cttctttatt ctacaaaaca ggtgtatcgc cttttggtaa tacagagtat
     6481 gaatactggc tcgacaataa ctatacgggc aggtcaacca tcagtaatac gtataccagt
     6541 aattttatcc tgctcgacag tatcatcacc attggtgtaa gtaatggcct gcacaccctc
     6601 cacttccgtt tccggcctga tggtaaaaca tggagcagcg tcaccagcag tttcttttat
     6661 aaagaccaga acagtattac cggcatcaac aacctggctc aatacgttta ctggtttgat
     6721 tctaactggc aaaacccgca aacgatcacc attaccggtg tgcaaaacct gaactggaca
     6781 ctgaatacca gtgtagatag tctatccaat ggtccgcatg ttctttcaca agcttttaaa
     6841 gatgataaag gacaatggag cagtattatt agtactgact tcaccaaaca acctgcttcc
     6901 actccttcct gtctttcttc tggccggcaa tttacagctg gcattatagc gggcggtgga
     6961 actgactacc agtggcagtt agataacggt accggcttta acgatatatt gaataatgcg
     7021 gtatataccg gcgccaatac agatacatta gaattgagca atgcccccac ttcctggtac
     7081 gggtataaat tccgttgccg ggtcaccaat ggcgccacca cattctacgg accaatgtac
     7141 acattaaaat ttgtatccac ctggaccggc actactgata atgcgtggga aaacccggct
     7201 aactggagct gtaatacaat acctgatgct aatacagatg tgtatgttaa tgccggaagt
     7261 gtaagatacc cggaagttaa ctcaactaca gctacctgca gaagtcttaa cctgcaacca
     7321 ggaaccagct ttgaagtgaa acccggattt actataaata ttactggaaa aaattaattg
     7381 aaaagctttt tactttttat actcttcgcc ctggttcgtg gaaatacaaa ccagggtata
     7441 gaaaaaaagc cggcaatgcc ggcttttttc acttcttcgg cgggttatta tctctaccac
     7501 cacctgaagg tctgcctgtt gtgctgggca agcccccatt accttttgct gcaacagatt
     7561 gtgctctttg atagaaaccg gttttaaagg tttttgaaaa ttgatttttt ccggcagcag
     7621 actggatacg gcccgcatct ctggttgtca tcattgattt cgctttcata ataaataatt
     7681 tagaaaccaa aactagcttt cagttaatgg gaatgctgct ttttccagtc aagtatagat
     7741 ttttcgaacg aaatgcgatg ctggtatgct tcctgtactt ttaagaagat ccggtaatac
     7801 tcttttgcag ctttgctggc agaaaattta aatggcgaca actcttttat ttctgaaaag
     7861 ggaagaatgg gttgcaattc cttttttcca gatagttggt aaaaggcgac atttattatt
     7921 acactatcag ataacattgc caggtaatgg ctcagcggat caaaaagatc cttcaaatca
     7981 tttaggtttt tat
//
LOCUS       DAORTI010000144         7788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_10482_length_7788_cov_8.196690, whole genome shotgun sequence.
ACCESSION   DAORTI010000144 DAORTI010000000
VERSION     DAORTI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10482_length_7788_cov_8.196690"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLO70_14165"
     CDS             <1..795
                     /locus_tag="PLO70_14165"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HQZ75663.1"
                     /translation="EGQYYDFDISDFLKKFNCTSHTTLYSLKALEQEDWISFNEQVFL
                     PSSVVFTVNKDSLYEFERSNAGLDPCIKILLRAYEGIFDQQTFISEKVIAGLMKKDAE
                     EVKKQLIELHRFRIIEYKPQKDTPQLYLLRNRIKAEDISINMAAYNKRKEQFQQRMKQ
                     MVEYVKEEAECRSRIIGSYFGDDSIRACGICDNCLRQKAAKLTREEFENLYHRIINMI
                     KFESLHTKDLLLKLNGIKKEKAWKVIEFLQAENKIEMDKNGWVRLK"
     gene            complement(787..2334)
                     /locus_tag="PLO70_14170"
     CDS             complement(787..2334)
                     /locus_tag="PLO70_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQZ75664.1"
                     /translation="MADSTKFLEVVKAGYTFKGEAFKIGCAMLDGHVVTGADVLLPFK
                     TLNRHGLIAGATGTGKTKTLQVLAEGLSDASIPVLMMDIKGDLSGIAAAGTVNDKIAD
                     RCQKLTIEYKPTAYPVDLLTLSEQKGARLRATVSEFGPVLLSKILGLNDTQGGFVAMI
                     FKYCDDNKLPLLDLKDFIKVLQFVSDEGKAELEKTYGKISTTSTGTILRKVIELQQQG
                     ADVFFGEKSFEVEDLMRINNEGKGMISIVRVTDLQDRPKLFSTFMLQLLAELYATLPE
                     EGDMDKPKLVMFIDEAHLIFQEANEALLQQIETIVKLIRSKGIGIYFCTQNPMDVPAS
                     VLAQLGLKVQHALRAFTAADRKVIKQTSENYPETDYYTTEDLITQLGTGEALVTILNE
                     KGIPTPLVHCMIRPPQSRMDILTDTEIDALVSRSKIAAKYNQVIDNKSAYEILTEKLE
                     AAAKREEAKKEQEAENKADKKPAKKEKGFFDDPTVRSMTRTAGNTIVRSLLGVLGLGG
                     RRRKSLF"
     gene            complement(2377..2748)
                     /locus_tag="PLO70_14175"
     CDS             complement(2377..2748)
                     /locus_tag="PLO70_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HQZ75665.1"
                     /translation="MHITPDNRLKKLIVIGDRMLIKPTRPDERTASGLYLPPGVQEKE
                     KVQQGYVIKTGPGYAIPMPVENESWKGEEEQVKYVPLQAKEGDLAIFLLSGATEVIYE
                     NEKYFIVPQSAILMLEREEDL"
     gene            2890..3585
                     /locus_tag="PLO70_14180"
     CDS             2890..3585
                     /locus_tag="PLO70_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75666.1"
                     /translation="MEAKKPKILLCEDDPNLGSVLKNYLELNDYDVTLERDGRLGLAA
                     FQREKFELCLLDVMMPNMDGFTLAEEIRDVDPDIPLFFLSAKTMKEDVIQGYKLGADD
                     YITKPFDSEVLLLKIKAILKRNEEQNKESENKEFDLSSYHFNPKLRQLIHNGVTQTLS
                     PKENELLKMLAEHLNDLLPREAALKKIWGSDTYFNGRSMDVYIAKLRKYLKDDEKIEI
                     VNIHGNGFRLVVQ"
     gene            complement(3652..4680)
                     /gene="ruvB"
                     /locus_tag="PLO70_14185"
     CDS             complement(3652..4680)
                     /gene="ruvB"
                     /locus_tag="PLO70_14185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQZ75667.1"
                     /translation="MANPNLNSDKQGLAAVDKEFENNIRPAAIADFSGQDQIIENLRI
                     FIKAAKIRGEALDHVLFHGPPGLGKTTLSRIVANELGVNIKETSGPVIEKPGDLAGLL
                     TSLEANDVLFIDEIHRLSTVVEEYLYAAMEDYRIDIMIDSGPNARSIQINLNPFTLIG
                     ATTRSGLLTAPLLSRFAIKSRLEYYTSAVLNKIIHRSAGILGTNISKEAVEEIGRRSR
                     GTPRIANGLLRRVRDFAQVLNDGQIDFGITQHALKALNVDEHGLDDMDNRILATIIDK
                     FKGGPVGITTIATAVGEEAGTLEEVYEPFLIQEGFLQRTPRGREVTAKAYEHLGKSRP
                     NTPGSLFS"
     gene            4738..5388
                     /locus_tag="PLO70_14190"
     CDS             4738..5388
                     /locus_tag="PLO70_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQZ75668.1"
                     /translation="MKIGVIGSGPVGRVLASAFLKEGYKVILGTRNISKEEMVNWQKE
                     NTDGLAGSFQETAQFGDIIVLAVSGLAVEDAIALAGKEHFFEKTVIDTTNPIAAIPPD
                     NGVLKYFTTLEDSLMERIQKILPDATKLVKAFNSVGNAFMYKPQFAGGTPTMFICGND
                     EGAKKTVTGILTSFGWETEDMGGVEAARAIEPLCILWCIPGFIRNQWKHAFKLLKA"
     gene            complement(5401..6105)
                     /locus_tag="PLO70_14195"
     CDS             complement(5401..6105)
                     /locus_tag="PLO70_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQZ75669.1"
                     /translation="MASLNILQEIHRYQAAFHPVVVFDHVKDKLLPFDFTAANKELTA
                     DILLSSNQFIAYINDKLNKANARYGIGGYAEHRTVYSSSKVFDGNTPGEEPRRLHLGI
                     DIWGPPLTAIMAPLDGIVHSFAFNNRFGDYGATIILTHLLGGFTFYTLYGHLSLHSIK
                     NLREGELVKRGEVFAEFGVPAENGSWPPHLHFQLILDIGEWKGDYPGVCKFSEKEQWV
                     ANSPDPDIILQLNQYL"
     gene            complement(6191..>7788)
                     /locus_tag="PLO70_14200"
     CDS             complement(6191..>7788)
                     /locus_tag="PLO70_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75670.1"
                     /translation="TPSGCTSSASVTVNATPPAPVVTPSSVSLCLGNIAPLSVPVTFN
                     SANGGAITINASGAGTPYPSTINVTGVPAGARVKSVRINGFAHTFPDDVDILLQSPAS
                     TNVVIMSDVGGSADNTGQNFVFDDAAGSLMSDGAFNASGTYRPTNYVTPDTWAAPGPG
                     AITQAVPTLSSFTGNMNGTWNLFVVDDLGGDGGAIASWSITFEVPGPVWSPATGLFTN
                     AAATIPYVAGTQLATVYASPATTTTYSVNNITPTCTSPTTTVTVTIYQPIAITTQPAS
                     QTVCAGATVTFSVATTGNFQTYQWQLSTDNGATWTNISGANATSYVVANTTTALSGRR
                     YRVIVTNSCNTVTSNAATLTVNALPTVVANDLFSRRICISDTLVPLIGTPVGGSWSGI
                     GVSGFNFIPGATAVGTYTLTYSYTNAAGCTSTDTTTVKVSDCPERIRLLRDNAVILFP
                     NPNNGQFNIRMNSVLYNYLGMKVYTAAGQLVHTQQWSGLVYGRVIPINLTHLPAAVYM
                     VRFVYDDGIRTSEKTFKVIVGRQ"
BASE COUNT         2262 a         1688 c         1574 g         2264 t
ORIGIN      
        1 gaaggacaat attatgattt tgatatttca gactttctga aaaaattcaa ttgcaccagc
       61 cacactacct tatattcttt aaaagcattg gaacaggaag actggatatc ttttaacgaa
      121 caagtttttc tcccttcttc ggttgtattt actgtaaata aagactcgct gtatgaattt
      181 gaaagatcca atgcaggatt agatccctgt attaaaatat tactccgggc atatgaaggg
      241 attttcgatc agcaaacatt catttcagaa aaagtaattg cagggctgat gaaaaaagat
      301 gcagaggaag taaaaaaaca gctgattgaa ctgcatcgct tccggataat cgaatacaaa
      361 cctcaaaaag atacacccca gctttacctg cttcgcaatc gcatcaaagc ggaagacatt
      421 tccatcaata tggcagcata taataagcgc aaagaacagt ttcagcaacg aatgaagcaa
      481 atggtggaat atgtaaaaga agaagcagaa tgccgtagcc gtatcattgg cagttatttt
      541 ggtgatgaca gtatcagggc atgtggaatc tgtgataatt gcctgcggca aaaagcagcc
      601 aaattaacca gggaagaatt tgagaacctg tatcaccgga tcatcaatat gatcaaattt
      661 gaatcactgc atactaagga cctgctatta aaactcaatg gtattaagaa ggaaaaagcc
      721 tggaaagtaa ttgaattcct tcaggctgaa aataaaattg aaatggataa aaatggctgg
      781 gtaagattaa aataggctct tacgccttct gccacccagg cccaatacac ccaacaggga
      841 ccggacaatc gtattcccgg ctgttcttgt catgcttcgt accgttgggt catcaaaaaa
      901 tcctttttcc ttcttggcag gtttcttgtc agccttattt tctgcttcct gctccttctt
      961 cgcttcctcc cttttggctg ctgcttctaa tttctccgta agtatctcat aagcactctt
     1021 gttatcgatc acttgattgt atttagcagc tattttagat ctgcttacga gagcatcaat
     1081 ttcggtatca gttaaaatat ccatccggct ctgcggcgga cgaatcatac aatgcaccaa
     1141 tggcgttgga attccttttt cattcaatat ggttaccaat gcctccccgg ttcccaattg
     1201 agtaatcagg tcttctgtcg tataataatc tgtttccggg taattctccg aagtttgttt
     1261 gataactttt ctatcagctg ctgtgaaggc ccttaaggca tgttgcactt tcaatcccaa
     1321 ctgtgccagt acggaagctg gtacatccat cgggttttgc gtacagaaat agataccaat
     1381 tccttttgaa cggataagct tgacgatggt ttcaatctgc tgtaataagg cctcatttgc
     1441 ttcctggaaa ataagatgcg cttcatcaat gaacatcacc agttttggtt tatccatatc
     1501 accttcttca ggtaatgtgg cataaagttc tgcgagtaat tgcagcatga acgtagaaaa
     1561 aagtttcggc cgatcctgca aatctgtaac cctgacaata gaaatcattc cttttccttc
     1621 attattgata cgcatcaggt cttcaacttc aaaacttttt tctccaaaaa acacatctgc
     1681 cccctgttgt tgcaattcaa tgaccttgcg aagaatagta ccggtagaag tggtggatat
     1741 tttcccgtac gttttttcca attctgcttt tccttcatca cttacaaatt gcagcacttt
     1801 tataaaatcc tttaaatcca gtaaaggcag tttgttatca tcgcagtatt tgaagatcat
     1861 tgccacaaag cccccctgcg tatcattcag tcccaaaatt tttgaaagca gcacaggtcc
     1921 aaattcactt actgtagccc gtaatcttgc tcctttttgt tcactcaatg taagcagatc
     1981 aacaggatat gcagttggct tatactcaat agttaatttc tgacaacgat ctgctatttt
     2041 atcatttaca gtacctgcgg cggcaattcc actcaggtca ccttttatat ccatcatcaa
     2101 tactggtata ctggcatcac ttaacccttc tgccagtacc tgcaatgttt tggttttacc
     2161 ggtacccgta gccccggcaa tcaatccatg ccggtttagt gttttaaaag gcagtaatac
     2221 atctgccccg gtaacaacat gaccatccag catcgcacaa ccaattttga atgcctcacc
     2281 tttgaatgta taacctgctt ttaccacttc caggaacttt gtactatctg ccatttggat
     2341 aattttgaat gaaagttaat cgaaaagttt tagttgttat agatcctctt ccctttccag
     2401 catcaggata gcactttgcg gcacgatgaa atatttttca ttctcgtaaa tcacttcggt
     2461 agcaccgcta agcaggaaaa tagccaggtc gccttctttt gcctgtaagg gtacgtattt
     2521 gacctgttcc tcttcgcctt tccagctttc attttctacc ggcataggaa tagcataacc
     2581 ggggcccgtt tttatcacat aaccttgctg cactttttcc ttttcctgta cacctggtgg
     2641 caggtagagt ccactggcag ttctttcatc tggccgggtg ggttttatca gcatccggtc
     2701 gccaattact attaattttt tcagcctgtt gtcaggagtg atatgcatgg cacagttttt
     2761 tattatatac agcaaaagta gaaagcaaag gggttgaaac aacggtgttt taacaaaata
     2821 ttcagcccgg atacttgaac ccccgaaatg ctttaacttt atttgtcact aatataaaac
     2881 caccagacaa tggaagctaa gaaacctaaa attttgttgt gcgaagatga tccgaatctt
     2941 ggaagtgttc taaagaatta cctggaatta aatgactatg atgttacgct ggaaagagat
     3001 ggccggcttg gcctggctgc ttttcaacgg gaaaaatttg aactctgcct gctggatgta
     3061 atgatgccta atatggacgg atttaccctt gcagaagaaa tacgggatgt ggaccctgat
     3121 attccgctct tcttcctcag cgccaaaaca atgaaggaag atgtgataca aggctacaag
     3181 ctaggcgctg atgattatat caccaaaccg tttgacagtg aagtgctgct cctgaaaata
     3241 aaagctatcc tgaaaaggaa cgaagagcag aataaggaaa gtgagaacaa agagtttgac
     3301 ctgtccagtt atcacttcaa tcctaaattg cgtcaattga ttcataacgg agtaacacaa
     3361 acattatctc ccaaagaaaa tgagttactg aaaatgctgg ctgaacacct gaatgatctg
     3421 ttgccaagag aagcagccct gaaaaaaata tggggcagcg atacttactt caatggccgc
     3481 agtatggatg tgtacattgc caagctaaga aaatacctga aagatgatga gaagattgaa
     3541 attgtgaata ttcatggtaa tggattcagg ctggtagtgc agtaagaaat gggctgatta
     3601 gaaaattttt aatataccca aatgagtgcc cctgaccagg gcattcattt tttaggagaa
     3661 taatgaaccg ggggtgttcg gacgggattt tcccaaatgc tcatacgcct tcgccgtcac
     3721 ttctctgcct cttggggttc gttgtaaaaa accttcctgt atcaaaaaag gttcatacac
     3781 ttcttccaat gtacccgctt cttctcctac tgctgtggca atggtggtga tgccgacagg
     3841 cccgcctttg aatttatcaa tgatggtggc aagtatcctg ttatccatat catccaaccc
     3901 atgctcatcc acattcaatg cctttaaagc atgttgtgtg ataccaaaat caatttgacc
     3961 gtcatttaat acctgggcaa aatctctaac cctcctgagc aagccatttg ctattctggg
     4021 tgtgccccgg cttcttctgc caatttcttc caccgcctct tttgaaatgt tagtacctaa
     4081 tatccctgca gatctatgaa tgatcttatt cagcactgct gaagtataat attccagtct
     4141 tgatttaatg gcaaatcttg ataataatgg agctgtgagc aacccactcc tggtagtagc
     4201 cccaattaac gtaaatggat tgagattgat ctggatgctt cttgcattgg gaccggaatc
     4261 aatcataata tctatgcggt aatcttccat tgctgcatac aggtattctt ccaccactgt
     4321 gctcaggcgg tgtatttcat caataaacag aacatcatta gcttccaaac tggtaagcag
     4381 gccagcaaga tcaccgggct tttctattac cggacctgaa gtttctttaa tattcacccc
     4441 cagttcattg gctacgattc tcgatagcgt tgttttcccc aaaccgggag gcccatgaaa
     4501 taaaacatgg tccaatgcct cgccacggat cttggcagcc ttaataaaaa tgcgcagatt
     4561 ttcaatgatc tggtcctgac cggaaaaatc tgcaatagca gcaggccgga tattattttc
     4621 aaattccttg tccaccgccg ccaggccctg cttatcacta ttgaggttag gattagccat
     4681 caattttttt tcttaatatt tgtaaagcta attattaaaa aagaaaaacg gacagttatg
     4741 aaaatcggag tcatcggttc aggacctgta ggccgggtat tggcctctgc ttttttaaaa
     4801 gaagggtata aagtaatact gggtacccga aatatttcaa aggaagaaat ggtaaactgg
     4861 caaaaggaaa atactgatgg cctggcaggt tctttccagg aaactgccca gttcggagat
     4921 attattgttc ttgctgttag tgggcttgct gttgaagatg caattgcgct ggcaggcaaa
     4981 gaacattttt ttgaaaaaac agtaatagat actaccaatc ctattgcagc aattccgccc
     5041 gataatggag tactcaaata tttcactaca cttgaagatt cacttatgga aaggatccag
     5101 aagattttgc cggatgccac caaactggta aaagctttca atagtgttgg taatgcattt
     5161 atgtataaac cacaatttgc cggtggtaca cccactatgt tcatatgcgg aaatgatgag
     5221 ggagctaaga aaacagtaac tggtatttta acatcatttg gatgggaaac agaagatatg
     5281 ggaggagtgg aagcagcgag agccattgaa cccctttgta tattatggtg cattccgggc
     5341 tttatccgga atcagtggaa gcatgctttt aaattactga aggcctaaac agatccatat
     5401 ctataagtac tggttcaact gtaaaataat atccgggtca gggctgttgg caacccattg
     5461 ttccttttct gaaaatttac aaacaccggg ataatcacct ttccattctc ctatatcaag
     5521 tatcaattga aaatgcaaat gaggcggcca gctgccattt tcagcgggga caccaaattc
     5581 tgcaaatacc tcaccccttt ttaccagttc tccttcccgg agatttttaa ttgaatgtaa
     5641 actcaggtgt ccgtataaag tataaaatgt gaagcctccc agtaaatggg tgagaataat
     5701 agtagcgcca taatcaccaa aacgattatt aaatgcaaaa ctgtgaacaa tcccatccag
     5761 tggcgccatt atagccgtta ggggtgggcc ccagatatca atccccaggt gaaggcgccg
     5821 gggctcttcc ccgggtgtat taccgtcaaa aaccttgctg ctactataga ctgttctgtg
     5881 ttctgcgtac ccaccaatac cataccgggc attcgctttg ttcaacttat cattgatata
     5941 agcgataaac tggttagagc tgagtaaaat atccgctgtc agttctttat tagcagctgt
     6001 aaaatcaaat ggaagcaact tatcctttac atggtcaaat accaccacag gatgaaaagc
     6061 tgcctggtac ctgtgtattt cctgcaaaat gttcaatgat gccattctct aaagataagt
     6121 ggacagctga caagaaaaaa ggcatcccga taatttcggg atgccttata attataaact
     6181 gaatctttct ttactgtctg cctactatta ctttgaaggt cttctccgat gttctgattc
     6241 catcgtcgta aacgaacctt accatgtaaa ctgcagctgg cagatgagtc aggttaattg
     6301 gtatcacacg gccataaacc aagccactcc attgttgtgt atgtaccagt tgtccagcag
     6361 ctgtgtacac tttcatacca agataattat acaatacact gttcatccgg atgttgaatt
     6421 gtccgttgtt cggattcggg aacagtatta ctgcattatc tctcaataac ctgattcttt
     6481 caggacaatc actcactttc actgtagttg tatcagttga tgtacaaccg gcagcattag
     6541 tatatgaata agtaagcgta tatgttccaa cagcagtggc acctggtata aagttgaaac
     6601 cagaaacacc aataccactc cagctaccac caaccggtgt tccaattaat ggaaccaatg
     6661 tatcactgat acagatcctc ctgctgaaca gatcgttcgc tacaacagtt ggtaaagcat
     6721 ttactgttaa tgttgccgca ttagaagtaa ctgtattaca actattagtt acaatcacac
     6781 gataccttct gccgcttaaa gcagtagttg tattagccac tacatatgat gttgcattag
     6841 caccactgat attggtccag gtagcgccgt tatctgtact taactgccat tggtatgtct
     6901 ggaagttacc ggtagttgct acactaaatg taaccgttgc accggcacaa acagtctggc
     6961 tggcaggctg tgttgtgatt gcaattggct gataaatagt aacagttacc gttgttgtcg
     7021 gtgaagtaca ggttggtgta atgttattta cggaatatgt tgtagtggtg gccgggctgg
     7081 cataaacagt tgccaactgg gtacctgcta cataaggaat agtggcggca gcgttagtaa
     7141 acagtccggt agctggtgac caaaccggtc cgggaacttc aaaagtgata ctccagctgg
     7201 caatagcacc tccatctccg cccaggtcat cgactacaaa caagttccag gtaccattca
     7261 tattaccagt aaatgaagat agtgttggaa ctgcctgtgt tatggcgcca ggtccgggag
     7321 ctgcccatgt atcaggtgta acataattgg ttggcctgta cgtaccagat gcgttgaagg
     7381 caccatcaga cattaatgat ccggctgcat catcaaatac aaaattctga ccggtattat
     7441 cagcactacc acctacatca gacattatta ctacgttagt gctggcgggt gattgcagca
     7501 ggatatctac atcatcaggg aatgtatgtg caaacccgtt gatacgaact gatttaactc
     7561 ttgctccagc cggtacacct gtcacattga ttgtggacgg gtaaggtgtt ccagcacctg
     7621 aagcattgat tgttattgca cctccatttg cagagttaaa tgtaaccggt actgacagag
     7681 gagctatatt tccaaggcaa agcgaaacac tgcttggagt aactactggt gccggaggtg
     7741 ttgcattaac tgtaacagat gcagaactgg tacaacccga aggagtaa
//
LOCUS       DAORTI010000145         7685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_10685_length_7685_cov_7.171691, whole genome shotgun sequence.
ACCESSION   DAORTI010000145 DAORTI010000000
VERSION     DAORTI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10685_length_7685_cov_7.171691"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLO70_14205"
     CDS             complement(<1..426)
                     /locus_tag="PLO70_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75671.1"
                     /translation="MRKIIVTSLALLIFYSSQAQSKSTNSSSYQTALGVKVWDGGGIS
                     LKHFINGNNALEVIGYFWRQGVRITGLYEIHGHISGAAGLQWYIGPGAHIGFYNTRNG
                     DGSFIGIDGVLGLDYKFKGAPINMSIDWQPSFEFGDNRGF"
     gene            complement(518..1717)
                     /locus_tag="PLO70_14210"
     CDS             complement(518..1717)
                     /locus_tag="PLO70_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ75672.1"
                     /translation="MRIITRTVLILSVVSLLADIASEMLYPVIPVYLKEIGFSVFLIG
                     VLEGVVQFTAGISKGYFGKWSDEMGFRLPFVKLGYFLSAVSKPLMATFTYPLWIFFVR
                     TIDRLGKGVRTAARDALLSREATKETKARVFGFHRSMDTAGAAIGPTVALLFLYFYPG
                     EYSTLFYIAFIPGMLSVFLIFLLKEKKNPVSTMGKGNFFSFFRYWKIANPEFRKIVPG
                     LLLFALFNSSDIFLLLITKETIGNNTITLLGSTFNADTITIAAYIFYNLVYALASYPM
                     GILADKLGFKRVVIFGLILFTIVYGGFAFKPSVPLIFILFSIYGIYAAATEGVIKAWI
                     TNLSHNQDTATAIGFYTSCESMSTLFASIIAGAIWTSLGSSYTFIITAVISLVVVLYF
                     SLIIKAK"
     gene            1942..2967
                     /locus_tag="PLO70_14215"
     CDS             1942..2967
                     /locus_tag="PLO70_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQZ75673.1"
                     /translation="MGLFNWFTQEIAIDLGTANTLIIHNDEVVVNEPSIVALNRNNPK
                     EVLAVGKKALMMHEKTHESIRTVRPLKDGVIADFNAAELMIRELIKLVYPKKPLFPPS
                     WRMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQVG
                     AAMKEIDNPPDDFAVNGRDLVTGIPKQIMVSYQEIAEALDKSIFKIEEAILKALEQTP
                     PELAADIYRRGLYITGGGALLRGLDKRLSNKIKLPVHIADDPLKSVVRGTGIALKHYD
                     RYPFVMR"
     gene            3042..3920
                     /gene="mreC"
                     /locus_tag="PLO70_14220"
     CDS             3042..3920
                     /gene="mreC"
                     /locus_tag="PLO70_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQZ75674.1"
                     /translation="MRNVFLFIRRYKTFFTFLLLQVVSLWFLYNYNKFHRAKFLGVAN
                     EMTGRINTQYNKVEDYFVLREENRRVHRMNDSLLNLLPQNFVRADTSVQLVQDSVLFD
                     TSGNFRRYYFRPAGVVYNTVNSQKNYIQLNRGLNHGIKDNMAVLSSDGSAVGVVVGVS
                     PNFCQVMSLLHVQQKVNVSLKKSGDFGTIEWDGKDPRLLTLRGIPKSIEIKTGDTVLT
                     STYSFNFPPGKLVGTIAEVVTDKSTNFYVLRVKPGARFYNLQQVFVVENLQYTEQTAL
                     NLESRKRIEDPKKNPK"
     gene            3917..4450
                     /gene="mreD"
                     /locus_tag="PLO70_14225"
     CDS             3917..4450
                     /gene="mreD"
                     /locus_tag="PLO70_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQZ75675.1"
                     /translation="MSDLLRNIIRFAVFISIQVYVLNKVPHLHRFITPYLYFLFILWL
                     PFSVSRQWLLVIGFFTGLALDYFTMTPGLHAAACLLIAYVRPFIINILTPKDSTEFNY
                     REPSPKAMQWTPYLVYVFILTFLHHGYMVFLQWLSFGSFLDFIVKVIATTGISMLLIV
                     TVELLFPRKLKFRTNTA"
     gene            4619..6715
                     /gene="mrdA"
                     /locus_tag="PLO70_14230"
     CDS             4619..6715
                     /gene="mrdA"
                     /locus_tag="PLO70_14230"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQZ75676.1"
                     /translation="MSVFNQSRSRIIRIIFLIAFVVIIAQLFYLQVIEGKYRQLAMDN
                     AVYAKVVYPERGIIYDRKGKAILNNTIIYDLMVTPAEVKGIDTMDFCRLIGIDTAEFK
                     KRILEARFKNTAVRPSIFKGLLTKEIQARFEENSWKYPGFNLVERPLRTYPFNAGAHI
                     LGYIREADTKDIENSGSFYRQGDYIGKTGLESYYEKVLMGQRGVEYMIKDNKNRLVGH
                     YENGAFDTASIAGRGLRTYIDVELQQLAEKLMTNKVGGLVAIDPKTGGILAMVSGPNF
                     NPNDLTGSDGSKNFSRMQLDVTGPMLNRAIGGKYEPGSTFKPLGALVALDEGVITPSY
                     GYPCGGRYTLCGHGKPACTHAGGGHAANVRLSIANSCNAYYAHVYRLAVDNPKYDNVK
                     LGYLKWKEYMNAFGLGVRLGIDLPNESAGGIPDTSVYNKENAGRWTSCTNLTLGIGQD
                     KMQTTPLQMANAMCIIANKGYYYTPHFVEKIDGETDEDTTLMNKFRVRHNVLTQIADT
                     AFNEVIEGMNDVVKFGTARNAQIPGIDVCAKTGTAENYTILDGRRIKLPNNSMFVCFA
                     PKENPKIAIAVFVQNAGFGSTWAGPISRIMIEKYLNDTLMTKSKADLERIASTDLMPA
                     YFKRLQYKEDSTRAFKWFQTTKDSSYIKKYISTLYGPVSPSPGKVPVPAKKEIRMLAI
                     LPADKNFRKPDLQKRS"
     gene            6712..>7685
                     /gene="rodA"
                     /locus_tag="PLO70_14235"
     CDS             6712..>7685
                     /gene="rodA"
                     /locus_tag="PLO70_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQZ75677.1"
                     /translation="MNQQNPAISKGIDWSLAWIYLLLVMIGLTAIFAATYKEGDAVVQ
                     SFINFKTDYSKQFYFFLIGAAIGFFILLTDSKFFAATANLWYAVGILLLLLVFPFHSR
                     VKGTESIIKLGAFNFQPAEFCKIFVALSLAKYLSRPETDFSKTRSQLIAAGIALLPAV
                     ITILQSETGLALVYFSFFIVMYREGLPSIVLIIGFSIAALVVATIVIEPNTLAITLTA
                     IAALIIYIFRRQIKRRRDLLVTIIFLWALCVGIQRFAVPVIFKNVLQKHQVERIYDLF
                     GKDNPYLEKGEDITAEEAKKKKESGSSYNVRQSKIAIGSGGFKGKGIL"
BASE COUNT         2325 a         1482 c         1654 g         2224 t
ORIGIN      
        1 aaatccgcgg ttatcaccaa attcaaatga tggctgccag tcaatagaca tattgattgg
       61 agcgcctttg aatttataat caagcccaag tacgccatca atcccgataa agctgccgtc
      121 accattccgg gtattgtaga aacctatatg agcaccgggt cctatatacc actgcagacc
      181 tgcagcaccg ctgatgtggc catgtatttc atacaagcct gttatgcgaa caccctgtct
      241 ccagaaatag ccgattactt ccagtgcatt attgccattg atgaagtgtt ttaatgaaat
      301 tcctccgccg tcccagactt ttacacctaa tgcggtttga taggaggagc tgttggtgct
      361 tttgctttgt gcttgtgaag agtaaaaaat cagtaatgcg aggcttgtaa caataatctt
      421 tctcatacta taattttaag ttgagttaac agtttcaagt ccctccttgc cattactatg
      481 ccaaaaacag gtaagcaata aagattaata actaaaatta tttagccttg attatcagtg
      541 agaaataaag taccacaact aatgagatca cagcggtgat gataaaagta taggaactgc
      601 ccaggctggt ccatatcgca cctgcgatta tactggcaaa taatgtactc atactttcac
      661 aggaagtgta aaatccaatt gcagtagctg tatcctggtt atgtgacaag ttagtaatcc
      721 atgctttaat gactccttcg gtagcagccg cataaatacc atagatggag aacaaaataa
      781 aaattaaggg tacagagggt ttaaatgcga agccaccata gactattgta aatagaatta
      841 atccgaaaat gacaactctt ttaaagccaa gtttgtctgc cagaattccc attggatagg
      901 atgcaagggc ataaacaagg ttataaaaaa tataagcggc aatggtgatg gtatcagcgt
      961 taaatgtact tcccagtagg gttatcgtat tattcccgat agtttcttta gtaatgagta
     1021 gtaaaaagat atctgagctg ttgaagagtg caaacaataa taaaccggga actatttttc
     1081 gaaactcagg atttgctatt ttccaatacc tgaaaaagga aaagaagttt cctttgccca
     1141 tagtggaaac agggttcttt ttttctttca ataagaaaat taaaaagaca gataacatgc
     1201 ccggtataaa agcaatataa aagagagttg aatattcgcc cggatagaag tataggaaca
     1261 ataatgcaac agttggtccg atagcagcgc cggcagtatc catactgcga tggaaaccaa
     1321 aaactctcgc ctttgtttcc tttgttgctt cccgggaaag tagtgcatct cttgcagcag
     1381 tgcggacacc tttgcccaac cggtcaatag ttcgtacaaa aaatatccat aaagggtaag
     1441 taaaagtggc catcaagggt tttgatacag cacttagaaa atacccgagc tttacaaatg
     1501 gtaaacgaaa ccccatttcg tcactccatt ttccaaagta acctttgcta atacctgccg
     1561 taaattgtac cactccttcc agtacaccga tcaggaaaac gctgaagccg atctctttta
     1621 aataaacagg aataaccgga tacagcattt cacttgcaat atctgccagt aaactaacga
     1681 cagacaaaat cagaactgtt ctggtaatga ttcgcatatg atgaaggtaa aaaaaaacgt
     1741 tgttgcctgt taagttaact attccgtgca acctgctatt tcttatacca aaaatttcct
     1801 gtaattacaa tgtttaaaat ccttttaact gcgaattatc taaattcact atataaaaaa
     1861 aatccatgcc agacaaggtt ttgctttgat aattcaaatt atttcctcca cctttgctca
     1921 caataaatcc gatataaatt catgggactt tttaattggt ttacacaaga aatagctatt
     1981 gacctgggta cagctaatac cctcatcata cacaatgacg aagtagtggt gaatgaaccg
     2041 agtattgttg ccttaaatcg taataatccc aaagaggttc tggcggtagg taagaaagcc
     2101 ctcatgatgc atgaaaagac ccatgaaagc atccgtactg ttcgtccgct gaaagatggt
     2161 gtgatagccg atttcaatgc tgcggagctg atgattcggg agcttatcaa actggtttat
     2221 cccaagaaac ctcttttccc cccaagttgg agaatgatga tctgtatacc ctcctccatt
     2281 acagaagtgg agaaaagggc cgttcgggac agtgcagaac aggctggtgc caaagaagtg
     2341 taccttattc atgaacctat ggctgcggca ctgggtatcg gtattgacgt agaagagccg
     2401 gtgggtaata tgatcataga tattggtggt ggcacaacgg gtataaccgt cattgcgttg
     2461 gctgggattg tttgcgacca gtcaatccgg atagctggtg atgaatttac tgctgatata
     2521 atggaggccc tgcgtcgtta ccatagttta ttgataggtg aacgaactgc tgagcaaatc
     2581 aaaatacagg tgggagctgc catgaaagaa attgacaatc cacctgatga ttttgcagta
     2641 aatggccgtg acctggtaac gggtataccc aaacaaataa tggtgagcta ccaggaaata
     2701 gccgaggcac tggacaaaag catctttaaa attgaagaag caatccttaa agcactggaa
     2761 caaacccccc cagagcttgc tgctgatatc taccgccgtg gtttatacat aaccggtggc
     2821 ggtgccctgc ttcgtggtct tgataagaga ttgagcaata aaataaaatt gccggtacat
     2881 atagccgatg acccgctgaa aagtgtggtg cggggaacgg gtattgcact gaaacattat
     2941 gacaggtatc cttttgtgat gcgataagaa cgaaatacaa agtacggtta acgacttaaa
     3001 acggaacgta cttcgtattt ttaactccaa acctgcaaca gttgcgtaac gtttttctct
     3061 ttatccgccg gtataaaacc ttttttacat tccttctatt acaggtggta tccttgtggt
     3121 ttttgtataa ctacaataag tttcaccggg ctaaattcct gggtgtagcg aatgagatga
     3181 ccggccgcat caatacgcag tataataaag ttgaggatta ctttgttctc agggaagaaa
     3241 atcgaagggt acaccggatg aatgattcct tgctgaattt actgcctcag aattttgtgc
     3301 gggcagatac aagtgttcag ctggtgcagg attctgttct atttgatact tcgggaaatt
     3361 tccgtcgcta ttatttcaga ccagccggag ttgtttataa caccgtcaat agtcaaaaaa
     3421 attatataca actgaaccgg ggcctgaatc acggaataaa agacaatatg gccgttttaa
     3481 gttcagatgg cagtgcagta ggggtagtgg taggggtcag tcctaatttt tgccaggtaa
     3541 tgagtttgtt gcatgtccaa caaaaagtaa atgtttcttt gaaaaagagt ggtgattttg
     3601 gcactattga gtgggatggg aaggatcccc ggttactgac attaagagga atacccaaga
     3661 gcattgaaat aaaaaccggt gataccgtac ttaccagtac ttattctttc aattttcccc
     3721 ccgggaaatt ggtaggcacc attgcagaag tggttactga caagagtaca aacttttatg
     3781 tactcagagt taagcccggg gcccgctttt acaatttaca gcaggtgttt gtagttgaga
     3841 acctccaata tactgaacag acagccctga acctggaaag ccgcaaaaga attgaagacc
     3901 ctaaaaaaaa tccaaagtga gtgatctgct gagaaatatc atccgctttg ctgtattcat
     3961 ctcaatacag gtatatgtat tgaacaaggt gccgcacctg caccgtttta taactcctta
     4021 cctctatttt ctctttatcc tttggttacc attttcagtt tcccgtcaat ggttgctggt
     4081 gattgggttt tttaccggcc tggcacttga ctattttaca atgacgccgg gccttcatgc
     4141 cgcagcctgt cttttgatcg catatgtccg tccatttatt atcaatattc ttacacccaa
     4201 ggatagtaca gaattcaatt acagggaacc ttcacccaag gctatgcaat ggacacctta
     4261 tcttgtctat gtttttattc ttacattcct gcatcatggc tatatggtct ttttgcagtg
     4321 gctgagtttt ggaagtttcc tggatttcat agtgaaagta attgctacta ccggtatcag
     4381 tatgctgctt attgtgacag ttgaattgct gttcccccgt aaattaaaat tcaggacaaa
     4441 tacggcctga cgagatattt acccttaact caggtataga aaattttctt aaatcaaatt
     4501 ttattttcaa atagatttgg aaacagactt ttgtaattcg tactttgtat aacgattaaa
     4561 aattcttttt ctcacctcat ccctggtaat tatcaggatt attgtttgtt gttgctccat
     4621 gtctgtgttt aaccaatcaa gaagccggat cattcgtatt attttcctta ttgcttttgt
     4681 agtaatcatt gcccagcttt tctacctgca ggtaattgaa ggaaagtaca ggcagttggc
     4741 catggacaat gctgtttatg ccaaagtggt ttaccctgaa agaggtatta tttatgacag
     4801 aaaagggaaa gcaatcttaa acaatacgat catttatgac ctgatggtaa cgcctgctga
     4861 agtaaaaggt atcgatacca tggatttttg ccggcttatt ggaattgata ccgctgaatt
     4921 taaaaaaagg atattggagg ccaggtttaa aaacacagct gttcgcccct ccattttcaa
     4981 ggggttactc acaaaagaga tacaggccag attcgaagag aacagctgga agtatcccgg
     5041 gtttaactta gtggaacgtc cattgcggac atatcctttc aatgcaggag ctcatatttt
     5101 aggttatatc cgtgaagcag atacaaagga tattgagaac tccggcagtt tttacaggca
     5161 gggagattat atcggaaaga cgggtttgga atcttattat gaaaaagtgc tgatgggcca
     5221 gcggggtgtg gagtacatga ttaaagacaa caagaacagg ctggttgggc attatgaaaa
     5281 tggtgccttt gatactgctt ctatagccgg aagaggatta cggacctata ttgatgtaga
     5341 attgcagcaa ttagctgaaa agctgatgac aaataaagtt ggcggactgg tagcgattga
     5401 cccaaagaca ggggggatac tggcaatggt gtcagggcct aatttcaacc ccaatgatct
     5461 taccggttcc gacggtagta agaatttcag ccgtatgcaa ctggatgtaa ccggcccaat
     5521 gcttaacagg gctattggag gaaagtatga acccggatcc acgttcaaac cattgggggc
     5581 actggttgca ttggatgagg gggtgattac tccttcatac gggtaccctt gcggaggccg
     5641 gtataccctt tgcggtcatg gtaaaccagc ttgtacccat gcgggtggcg gtcatgctgc
     5701 caatgtccgc ttatctatcg ccaactcctg caatgcttat tatgcacatg tgtatcggtt
     5761 ggcggtcgat aatccgaaat atgacaatgt aaaactcggt tacctgaaat ggaaagagta
     5821 catgaatgct tttggtttgg gtgttcgttt gggaatagat ctgcctaatg aaagcgcagg
     5881 tggtataccg gatacctcag tttataataa agaaaatgcg ggtagatgga cttcctgtac
     5941 caatcttaca ttaggtatcg gccaggataa aatgcagacc acccctttac aaatggcgaa
     6001 tgcgatgtgt atcatcgcta ataaaggata ttattataca ccacattttg tagagaaaat
     6061 tgatggtgaa acggatgaag atactaccct gatgaataag ttccgggtaa ggcataatgt
     6121 acttactcag attgctgata ctgcttttaa tgaggtcatt gaagggatga acgatgttgt
     6181 aaaatttggt accgccagga atgcacagat ccccggcatt gatgtgtgtg ctaaaacagg
     6241 cacagccgaa aactatacta tacttgacgg cagaaggatc aaattaccaa acaactccat
     6301 gtttgtatgc tttgcgccaa aagaaaatcc taaaattgct attgctgtat ttgtacaaaa
     6361 tgctggtttt ggatctacct gggccgggcc aatttccagg atcatgattg aaaaatacct
     6421 gaacgataca ctgatgacaa aaagtaaagc agacctcgaa agaattgcca gcactgatct
     6481 gatgcccgcc tattttaaac gacttcagta taaagaagat tctacccggg cctttaaatg
     6541 gtttcaaacg actaaagaca gtagttatat taaaaaatat atttcaactt tatacggtcc
     6601 cgttagcccg tcacctggta aagttcccgt acctgcaaag aaggaaatta ggatgctggc
     6661 aatattaccg gcagataaaa atttccgtaa accagatttg caaaaaagat catgaaccag
     6721 caaaaccctg ccatatcaaa aggaattgac tggagtttag catggattta cctgctgctt
     6781 gtaatgattg gtcttacggc catttttgca gccacctata aggaagggga tgctgtggta
     6841 caaagtttta ttaatttcaa aacagattat agcaaacaat tttatttctt tctgattggt
     6901 gcggcaatag gcttctttat tctgctgaca gacagtaaat tttttgctgc aacggctaac
     6961 ctctggtatg ccgttggtat tctgttgttg ttattggtat tcccttttca ctcaagagtc
     7021 aaagggaccg aatcaattat taagctggga gcttttaatt tccagccagc agagttttgt
     7081 aagatttttg tagccttatc attagcaaaa tatttatcca gaccggaaac agacttttcg
     7141 aaaacccgtt cacagttgat tgcagccgga attgcacttt tgccagctgt cattaccata
     7201 ttacaaagtg agaccggcct tgcattggtt tatttttctt tttttattgt tatgtaccgt
     7261 gaaggacttc cttctatcgt gctgataata ggtttttcaa ttgctgcatt agtagtggct
     7321 accattgtga ttgagcccaa tactcttgca attaccctga cagcaattgc cgcattgatt
     7381 atctatatat ttcgccgcca gattaagcgc cggcgtgatt tgttagtgac gattatcttt
     7441 ttgtgggctt tatgtgtggg tatccagcgg tttgctgtac cggttatttt taaaaatgta
     7501 ttacagaaac accaggttga acgtatctac gatttgtttg gaaaggataa tccttatttg
     7561 gaaaaaggag aagatattac tgcagaagaa gcaaaaaaga agaaggaaag cggcagcagt
     7621 tataatgtaa gacaatctaa aattgctatt ggcagtggtg gctttaaagg gaaagggatt
     7681 ttaaa
//
LOCUS       DAORTI010000146         7550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_10960_length_7550_cov_6.472315, whole genome shotgun sequence.
ACCESSION   DAORTI010000146 DAORTI010000000
VERSION     DAORTI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_7550_cov_6.472315"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLO70_14240"
     CDS             complement(<1..854)
                     /locus_tag="PLO70_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQZ75678.1"
                     /translation="MEIIHVAAECYPVAKAGGLGDVVGALPKYQAELGHVAKVVMPMY
                     RTKFLYNNDWELVHEGNQHIGPQYFNYSVIKEKTNKLGFDLYLVDINGLLDREKIYGY
                     EDDIERFLAFQVAVCDWMNKWQHTPDVIHCHDHHTGLIPFMVKYCFEFRQKLAAIPTV
                     FTVHNAQYQGWIGWDKYYLLPSFDSWKWGMLDWNNSINPMASAVKCAWKVTTVSHSYL
                     DELKETANGLENLFQYELGKSSGILNGIDNDVWDPVTDSMIAKNYSKELVEKGKKKNK
                     KELAGQFGL"
     gene            1076..2734
                     /locus_tag="PLO70_14245"
     CDS             1076..2734
                     /locus_tag="PLO70_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ75679.1"
                     /translation="MSQPKQPTNYGALGTLVTVFFFWGFIAAGNSVFIPFCKHYFHLD
                     QFQSQLIDFAFYTAYYIGALILFAYGAFGGKDLVGKWGYKNSIVYGLLFSALGAAAMI
                     VAVNGNSFAGMLIGLFIVALGFSLQQTAAQPFAISLGDPATGTSRVNLGGGVNSFGTS
                     VGPLIVALALFGTTAAITDEKIASLSLSKVIVLYTFVGILFVAAAALFYFSKKVPSGK
                     MIESTEKASKALYTLLIMTGLLIVMFVPVFSSYKTDLTNASDAEKHAMEAYRLKWLIG
                     ALAVVVLGLIFSNLSAKKKADGWGPMQYPQLVLGMLAIFVYVGVEVAIGSNLGELMKQ
                     KSFGSHSASEATPYIIMYWGSLMIGRWTGAISVFKLSEQTKNILRFIVPLVAFGILIG
                     INTLAKYDMTPLYYYVVCVLIQIAAFYLSKDKPARTMLIFSLLGIIAMVVGLMTTGTV
                     AIYAFLSGGLVCSIMWPSIFSLSIAGLGKYTSEGSAFLIMMILGGGIIPPIQGKLADI
                     IGIHQSYIIAALCFGYLAFFAFAVKGILKKQGIDYDAEVSAGGH"
     gene            2760..3716
                     /locus_tag="PLO70_14250"
     CDS             2760..3716
                     /locus_tag="PLO70_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQZ75680.1"
                     /translation="MAEFAIGIDIGGTNTKFGVVDRYGEIIEQGRASTNEHENVQEFI
                     DELFVQLSPMIKKAGGIKEFVGIGIGAPNGNYYTGNIEHAANLKWKGIIPLASMMSEK
                     FGLKVKLTNDANAAAVGEMMYGCAKGMKHFITITLGTGVGSGIVIDGKIVLGHDGFAG
                     ELGHVVVRPGGRLHKGTGIRGSLEVYASATGVRETALEILSEHPETESLLRNYTMNEL
                     TSQTVYDCAVKGDKLALQIFEFTGQILGEALANFIMFSSPEAIILFGGLTKAGDLIIN
                     PTRKAMEENLIQVFKNKVKLMFSELKEADAAILGASALVWED"
     gene            complement(3789..4316)
                     /locus_tag="PLO70_14255"
     CDS             complement(3789..4316)
                     /locus_tag="PLO70_14255"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HQZ75681.1"
                     /translation="MKKIILSFFVLASLGAVVLVACSKDQSSGNNSTTLKIRLTDNPV
                     NAEEVNVDIQQVRVKFQEDSINGWMDLDTHTGIYDLLGLQNGVDTLLAVGVIPTNTVK
                     EIRFVLGSANSIKVGGITYPLTIPSGSESGLKIKVNKQLNGTLDSLLIDFDAALSIHQ
                     TGAGVYQLKPVLKIK"
     gene            complement(4418..5407)
                     /locus_tag="PLO70_14260"
     CDS             complement(4418..5407)
                     /locus_tag="PLO70_14260"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQZ75682.1"
                     /translation="MKKIFFTGAIVLLVAPFCFAQLKWQNVDSLFQPLPPSVHVYKTT
                     DLLDGKPNIAYYVEADLKDKKLDFTVDTTYKRRLKPSQFYEKNNQPLLVVNTTFFSFA
                     TNQNLNVVIKKGKMIGYNIHTISGKGKDTLTYRHPFGSAIGISKKREAYVAWVYTDSS
                     LRFAYATESVFQPVRDSIAIFPFQGFLKQFPQLDGVTRQGLKNHTRWKMKTAVGGGPV
                     LLQNGDIKITNNEELKFAGKAINDKHPRTAMGYTGDNKLVILVIEGRNPGKAEGATLI
                     QQAQIFKDLGCREALNLDGGGSSCMLVNGKETIKVSDASGQRPVPAVFIIRNK"
     gene            complement(5411..6343)
                     /gene="lipA"
                     /locus_tag="PLO70_14265"
     CDS             complement(5411..6343)
                     /gene="lipA"
                     /locus_tag="PLO70_14265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQZ75683.1"
                     /translation="MIELPVVPNNSEKETRVKKPDWLRVKLPIGESYKHVRGLVDNHK
                     LHTICESGNCPNMGECWGAGTATFMILGNVCTRSCGFCSVATGRPESVDWDEPQRVAE
                     AIHLMKVKHAVITSVDRDELPDGGSIIWHNTIRAVKSLNTDTTLETLIPDFKGKKEDI
                     QRVIEAAPEVVSHNIETVERLTRQVRIQAKYWRSMEVIRTLKEGGMRTKSGIMLGLGE
                     QKEEVIQTLKDLQANGCDVVTIGQYLQPTKRHLPVHRFVHPDEFAEYREIGYSIGLDY
                     VESGPLVRSSYHSEKHVFAGIGRQEWQNQREQQA"
     gene            complement(6362..7177)
                     /locus_tag="PLO70_14270"
     CDS             complement(6362..7177)
                     /locus_tag="PLO70_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75684.1"
                     /translation="MKKLSLLLLFAFVVISVSAQDTTTRRTGKENKKEARRQKVNNLI
                     RQAEEGVLVYQKQSIFGFQARTNGYGFFYELGRMKTNRKTNIYRIDITEIKHQKEDKV
                     QNAANGFIFFGNPYIYGKVNNFYQVTLGFGQQHILGQKGNKNGVAVSAIYNGGLAIGL
                     LRPYYLEVQDPLTSEVKIIKYSSADSALFLGPTIVGGGGLGKGWGEMKMKPGAFVKTA
                     LRFDYGRFNEVVSGIEVGLSGEFYASKIPIMASQKDRQFFFQAYIALLFGRRK"
     gene            7230..>7550
                     /locus_tag="PLO70_14275"
     CDS             7230..>7550
                     /locus_tag="PLO70_14275"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQZ75685.1"
                     /translation="MTSTVVYNGDLRTTCTHLHSSSSFETDAPIDNNGKGERFSPTDL
                     MSTSLATCMVTVMGIKARTMGFDLNEVKIDVLKIMKADPRRVGRIELTFHIPDSLKDI
                     DEKTK"
BASE COUNT         2153 a         1625 c         1491 g         2281 t
ORIGIN      
        1 agcccaaatt gtccggccaa ttctttttta tttttctttt ttcctttttc aactaattct
       61 ttgctataat tctttgcgat catcgaatcc gtaacaggat cccatacatc attgtctata
      121 ccatttaaaa taccggagct ttttcctaat tcatactgga aaaggttttc caggccgttg
      181 gcagtttctt ttaattcatc tagataactg tgacttaccg tggttacttt ccaggcacat
      241 tttacagctg aagccatggg attaattgaa ttattccagt ccagcatacc ccatttccag
      301 gaatcaaatg aagggagtaa ataatattta tcccaaccga tccaaccctg gtattgagca
      361 ttgtgcacgg taaaaacagt tgggatagct gccaattttt gcctgaactc aaaacaatac
      421 ttcaccatga aaggaatgag gcccgtatgg tgatcgtggc aatgaattac atccggtgtg
      481 tgttgccatt tattcatcca gtcgcaaacg gctacctgga aggcaaggaa ccgctctata
      541 tcatcctcat aaccataaat cttttccctg tcgagcaaac cgttaatatc aaccaggtac
      601 aaatcaaatc caagtttatt ggttttctct ttgatcacac tgtaattaaa atattgcggt
      661 cctatatgct gattgccctc atgcaccaat tcccagtcat tattataaag aaatttagta
      721 cggtacatcg gcattactac tttggccaca tggcccagtt cagcctggta ctttggtaaa
      781 gcccctacca catcacccag gccaccggct ttagccaccg ggtaacattc cgccgctaca
      841 tgaattatct ccataaattt tgataccaat atttaaacgg aaatttaagt gattattttc
      901 attgccaaca tgataaaaaa gaaaaatacg gctgacattt tatctttttt tgaaataccc
      961 ctaattctgt ttaacctttt ttcggacaac ctgtcaacaa tgctaacaag tgttttttta
     1021 ccggaaatga aaattgttat actttcagca ctatttataa aattaatcga ttgctatgag
     1081 tcaacccaaa caaccaacga attacggagc attaggtaca ttagtcacag tcttcttctt
     1141 ctggggcttt attgcagctg gtaacagtgt attcattccc ttttgtaaac attacttcca
     1201 tcttgaccag ttccaaagcc agttaattga ctttgccttc tatacggcat attatattgg
     1261 tgccttaata ttatttgcat acggtgcatt tgggggtaaa gaccttgttg gtaaatgggg
     1321 ttacaaaaac agtattgtat acggtctcct tttctcagca cttggtgcag ccgccatgat
     1381 tgtggcagtg aacggaaaca gtttcgcagg aatgctgatc ggacttttta ttgttgcgct
     1441 tggattttcc ctgcagcaaa cagccgccca gccttttgcc atttccttag gggatccggc
     1501 tacaggtacc agtcgtgtta atctcggggg tggcgtcaat tcgtttggta cttcagtggg
     1561 tccattaata gtagcacttg cattatttgg aactactgct gctattacag atgagaaaat
     1621 cgcttcattg agtctttcaa aagtgatcgt cctttacaca ttcgtaggta tcctgtttgt
     1681 agcagccgca gccctgtttt atttttccaa aaaagtccca tccggaaaaa tgatagagag
     1741 tacggaaaaa gccagtaaag cactatatac attattgatc atgacaggat tgttgatagt
     1801 gatgtttgtt cctgttttta gcagttataa aacagacctt accaatgctt ccgatgccga
     1861 aaagcatgct atggaagcct accggcttaa atggctgatc ggggctttgg ctgttgtagt
     1921 gttaggatta atattttcaa atctcagtgc caagaaaaaa gccgatggct ggggacccat
     1981 gcaataccct cagttggtat taggcatgct tgccatcttt gtttatgttg gcgttgaggt
     2041 tgccattgga agtaatctcg gcgagctgat gaaacaaaaa agtttcgggt cccattctgc
     2101 atctgaagca accccctata tcattatgta ctggggtagc ctgatgatcg gccggtggac
     2161 aggtgctatc agtgttttca aattatcgga acaaacaaaa aatatcctga ggttcattgt
     2221 tcctttagtt gcctttggta ttctcattgg tattaacaca cttgctaaat atgacatgac
     2281 tcccttgtac tattacgttg tttgtgtatt aatacaaatt gcagctttct acctgagtaa
     2341 agacaaacct gcccgtacaa tgctgatatt tagtttattg ggcattatcg ctatggttgt
     2401 cggtttgatg acaacaggta cagtggcaat ttatgcattc ttaagtggcg ggcttgtatg
     2461 ctccatcatg tggccgagta tattcagtct ttccattgcc gggttgggaa aatacacatc
     2521 agaagggtca gcgtttttga tcatgatgat attgggtggt ggtattatcc cacctatcca
     2581 gggaaaactg gcggatataa tcggtattca tcaatcttat attattgcag cactatgctt
     2641 tggctacctt gctttctttg ctttcgcggt aaaaggtata ctgaagaaac agggtatcga
     2701 ctacgatgct gaagtaagtg caggcggaca ttaaactatt atatctcaaa taaataatta
     2761 tggcagaatt tgcaattgga atagacattg ggggaaccaa cacaaagttt ggtgtggtag
     2821 accgttatgg agaaattatt gagcagggaa gagcatcaac aaatgaacat gaaaatgtac
     2881 aggagtttat tgatgaactg ttcgtacaac tttctcccat gataaaaaag gcaggcggca
     2941 ttaaagagtt cgttggtatc ggtatcggcg cccccaacgg caactattat accgggaata
     3001 ttgagcatgc agctaacctg aaatggaaag gtataatacc tttggccagt atgatgtctg
     3061 aaaaattcgg actgaaagtt aaactgacca atgatgccaa tgccgcagct gtaggtgaaa
     3121 tgatgtatgg atgcgccaag gggatgaaac atttcatcac cattacactg ggaaccggtg
     3181 taggaagcgg tatagtgatc gatggtaaaa ttgtgctggg ccatgatgga tttgccggtg
     3241 aactgggaca tgtggttgtg agacccgggg gcaggctgca taaaggaacc ggcatcaggg
     3301 gatcactgga agtatatgcc tctgcaacag gtgtacggga gacagcgttg gaaatacttt
     3361 cagagcaccc ggaaactgaa agcctgctta gaaattatac catgaatgaa ctgacaagcc
     3421 agacagttta tgattgtgca gttaaaggag ataaattagc attacaaata tttgaattca
     3481 cgggtcagat attaggtgaa gccctggcca attttatcat gttttcttca cctgaagcga
     3541 ttattttatt tggtggatta accaaagcag gagacctgat aatcaatccc acccggaaag
     3601 caatggaaga aaacctgatc caggttttta aaaacaaagt gaagctgatg ttcagtgaac
     3661 taaaagaagc cgatgcagct atacttggcg caagtgccct ggtgtgggaa gattaaatca
     3721 ctttcctgat cataaaaatt aaaagcccgg agtaaccggg ctttttagtt atcatagaaa
     3781 tgtgatcgtt attttatttt cagcacaggt ttcaactgat aaacacctgc acctgtttga
     3841 tgaatagaca gggcagcgtc aaaatcaata agcaatgaat ccaaagttcc attcaactgc
     3901 ttgtttacct tgatctttaa accagactca gagccactgg gaattgtcaa aggataagtt
     3961 atcccgccga ctttaattga attagcagaa cctaatacga aacggatctc tttcaccgta
     4021 ttggtaggga tgactccaac tgccagtagt gtatcaacac cattttgtaa acccagtaaa
     4081 tcatagatgc cggtatgtgt atcaagatcc atccaaccat taatggagtc ttcctggaat
     4141 ttaaccctta cttgctgtat atctacgttt acctcttcgg cattaacggg attatcagtc
     4201 aaccggattt ttaaagtggt gctgttatta ccagatgact gatcttttga acaggctacc
     4261 aataccacag ctcccaggga agccaataca aaaaacgaca gaattatttt tttcatactt
     4321 taaaaatttt ataccctata tgtatgaaaa ccatgccaaa catgatctta taccagtcat
     4381 caaaaggcat agttttccac aaacgcaaat ttactatcta tttattcctg attatgaata
     4441 ctgcagggac aggccgctga ccgcttgcat ctgatacttt tattgtttct tttccattca
     4501 ccagcataca gctgctacca ccaccatcca ggttaagtgc ttcccggcaa ccaagatctt
     4561 taaagatctg cgcttgctgg ataagagtgg cgccttctgc tttcccggga ttacgtcctt
     4621 cgatcactaa aataacgagt ttgttatccc ctgtatatcc catagctgtg cggggatgtt
     4681 tatcattgat agctttaccg gcaaacttca gttcttcatt gttggtaatt tttatatcgc
     4741 cattttgcaa aagaaccggg ccaccaccaa ctgcggtctt cattttccag cgggtgtgat
     4801 tttttaaacc ttgcctcgtt acaccatcca attggggaaa ctgtttcaaa aagccctgaa
     4861 atggaaatat agctattgaa tccctcaccg gctgaaaaac tgattctgtt gcgtaagcaa
     4921 atcgcaagga agaatcagta tacacccagg ccacatatgc ctctctcttt tttgaaattc
     4981 caatcgcact tccaaacgga tgcctgtatg ttaaagtatc ttttccttta ccggaaattg
     5041 tatgaatatt gtatccaatc attttacctt tctttatcac cacattcaga ttctgattag
     5101 tggcaaaaga aaagaaagta gtattgacca ctaagagtgg ttggttattt ttttcgtaaa
     5161 actgcgaagg ttttaatctt cttttataag tggtatcaac cgtgaagtcc agtttcttat
     5221 ccttcagatc ggcttctaca taataagcaa tattcggttt gccatccagt agatcagtcg
     5281 ttttatatac atgtactgat ggtggtaatg gctgaaataa tgaatcaaca ttctgccact
     5341 ttaactgtgc gaaacaaaaa ggagcaacca gtaatacgat tgctcctgta aaaaatattt
     5401 tcttcatata tcaggcttgc tgttctcttt gattttgcca ttcctgccgg ccgatacctg
     5461 caaacacatg tttttcgcta tgataagatg aacgtactaa tggaccgctt tctacgtaat
     5521 ccaatccaat gctatatccg atctccctgt attcagcaaa ttcatcagga tgtacaaaac
     5581 gatgaacggg taaatgcctt ttggtgggtt gtaaatactg cccgatagtt actacatcac
     5641 aaccattggc ctgcaggtct tttaaggttt gaatgacttc ttccttttgc tcacccagtc
     5701 ccagcatgat accacttttg gtacgcatgc caccctcttt caatgtacgt atcacttcca
     5761 tacttctcca gtacttggcc tgtatacgaa cctgccgggt cagtctttcc actgtttcga
     5821 tgttatggct aaccacttct ggtgctgcct caatcactct ttgtatatct tccttctttc
     5881 ctttaaagtc cgggatcaat gtctcgagag tggtatccgt atttaaagat tttacagctc
     5941 tgatagtatt atgccagata atagaacccc catcaggtaa ttcatccctg tctacagaag
     6001 taatcaccgc atgtttcact ttcatcaggt gaatggcttc tgccacccgt tgtggttcat
     6061 cccaatcaac tgattcgggc ctgccggtgg caacagaaca aaaaccacaa ctgcgggtac
     6121 acacattccc taatatcatg aaagttgcag taccggctcc ccagcactca cccatattcg
     6181 ggcaattgcc actttcacaa atggtatgta atttatgatt atctaccaac ccacggacat
     6241 gtttgtaact ttcgccgatg ggaagtttga cacgaagcca atcgggtttt ttcacccttg
     6301 tctctttctc actattatta ggaactacag gaagttcaat cattgagcaa aaataagacc
     6361 attacttgcg ccgtccaaac agcagggcaa tataggcctg gaagaagaat tgccggtctt
     6421 tctgactggc cataatagga atttttgaag catagaactc accactaagg cccacctcaa
     6481 taccagatac cacttcatta aatcggccgt aatcaaaccg cagtgccgtt tttacaaaag
     6541 ctcccggttt cattttcatt tcaccccagc ctttgccgag cccgccacca ccaactattg
     6601 taggccccag gaaaagggca ctgtctgcag atgaatactt aattatttta acctcactgg
     6661 taagaggatc ctgtacttcg agataatatg gcctcagcaa gccaatggca agaccgccat
     6721 tatatattgc agaaacagct actccgttct tattcccttt ctgaccaaga atatgctgct
     6781 gcccgaatcc gagggtgacc tggtaaaaat tattgacctt cccgtagata taagggttac
     6841 caaaaaagat aaacccattt gcagcattct gtactttatc ttctttctga tgctttattt
     6901 cggtaatgtc aatcctgtaa atatttgttt tccggttggt cttcatgcgg cccagctcat
     6961 aaaaaaaacc gtagccgttg gttcttgcct ggaatccaaa aatgctttgt ttctgatata
     7021 ccagtacacc ttcttcagcc tggcggatga gattattcac tttttgacga cgggcttcct
     7081 ttttattctc tttaccggta cggcgggttg tggtatcctg tgctgacaca gatataacaa
     7141 cgaaggcaaa aagtagtaac aaactgagtt ttttcacgga gatacttttt agtaatactt
     7201 aaacgtaaat ttagggcaaa atattgataa tgacttccac cgtagtttat aacggcgacc
     7261 tgagaaccac ctgtacccat ttgcacagca gttcctcttt tgaaaccgat gcacctattg
     7321 ataacaatgg aaaaggtgaa cggttctctc caaccgacct gatgtcaacc agccttgcca
     7381 cctgcatggt aacggttatg ggtattaagg ccagaacaat gggatttgac ctgaacgaag
     7441 tgaaaattga tgtattgaaa attatgaaag cagatccccg gagagtgggc cgtatagaat
     7501 taacatttca tatacccgac tcgctgaaag atattgatga aaaaacaaaa
//
LOCUS       DAORTI010000147         7541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_10982_length_7541_cov_7.938552, whole genome shotgun sequence.
ACCESSION   DAORTI010000147 DAORTI010000000
VERSION     DAORTI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10982_length_7541_cov_7.938552"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1723
                     /locus_tag="PLO70_14280"
     CDS             449..1723
                     /locus_tag="PLO70_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQZ75686.1"
                     /translation="MPDRKQAPAIVDAVNFKLNLKPYQHFVLDNGVEVYAVDAGAEEV
                     MLTEWVFFAGNTQEEQNLVAATTNFLLRNGTTKRTAFQINEHFEYYGAYLNRSCYNET
                     ATITLHCLTKHIDELLPVVKELITGSTMPSEELAIYQQNMKQRLKVNLKKSDFVAGRL
                     IDAYLYGEKHPYGKYSSAKEFDVLERDQLLGFYSQYYQQGKLMLFVAGKLPAHLDTLM
                     NRFFGDLPNNPVEVKHPALHPATDKKIRITNDAAGVQGSIRIARPFPNRHHPDFLKVQ
                     VLNALFGGFFGSRLMSNIREDKGYTYGIHSYLQNHIQHSAWMVSTEAGKDVCDAAITE
                     VYKEMKDLREELVEEEELLLVRNYMMGGILGDLDGPFQIIARWKNIILNGLTEQYFYD
                     SINTIKTISAEELQGLAQKYLQPEDFYELVVV"
     gene            complement(1733..2884)
                     /locus_tag="PLO70_14285"
     CDS             complement(1733..2884)
                     /locus_tag="PLO70_14285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75687.1"
                     /translation="MKNLFFALFITSFFSCSLEAQDLNWNTSAIPNNSLSFNFGSIGS
                     PASAVTYAVTGPGTITAGPTRFTTAQGDITWRTAITFAAVSDLKVYTLTFNPAVCGLS
                     FILYDIDGNNTSGDRAILNANYLGSPQTITMTALDPGFPGGPPTITGSGTATATGTGT
                     QGNQTDDRVQISIPGCTSSLTIQYGNNPAGAAGGRSFSIGNLNWTGSTLPVTFTHFSG
                     QKIAEGIISLKWKSENEINVSKYEIERSIDGINFVTAGFINTLSSDGNYAFTDNTPTK
                     GVLFYRIKEIDLDARFQYSSVIALRFAEGNRPGITVFPSPASQYIYITTDNNSLLNKI
                     MIFTSDGKMMLQQNGPGNKIDISNFPTGLYRVKTISKSGEVSLSSFMKR"
     gene            3045..>5013
                     /locus_tag="PLO70_14290"
     CDS             3045..>5013
                     /locus_tag="PLO70_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HQZ75688.1"
                     /translation="MTFDRKDLSDEQLVHFYRSILWPRMIEEKMLVLLRQGKISKWFS
                     GIGQEAIAVGATLALQEDEWIMPLHRNLGVFTTRNMPLSKLFMQWQGAQEGYSKGRER
                     SFHFGNREHHICGMISHLGPQLAIADGVALAYKLRHGSTGSPLKVSLAFTGDGGTSEG
                     DFHEALNTAAVWDLPVIFIIENNGYGLSTPTNEQYRCISLIDKAKGYGMEGVQIDGNN
                     ILSVYDTIKGVRDYCIKNQKPYLIECLTFRMRGHEEASGTRYVPQDLFKLWEMKDPIK
                     NFEEFLVGAGVLSEMKVAAIRNEFKEKIESELATGSTIKPVVADTEVELEDVYAEKFD
                     VRGLKFDVKIIDNSKINHELEVQTKDSRLIDSIKEGLHQSMQQHPNLILMGQDIAEYG
                     GAFKITEGFVNEFGKERVRNTPLCESAIVGAALGLSLEGYKSMMEMQFADFVTVGFNQ
                     IINNLAKIHYRWGQHADVVIRMPTGGGVGAGPFHSQSNEAWFVHTPGLKVVYPSTPMD
                     AKGLLIAAINDPNPVLFFEHKALYRSVSGPVPSEYYEIEIGKARHVREGEEVSIITYG
                     AGVLWADDYAAEHPEISIDILDLRTLLPLDYFAIREAVQRTGRVLLLHEDTLTGGIGG
                     EIAAWIAENCFGLLDAPVMRCASLDTPIPFNI"
     assembly_gap    5014..5113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5114..6379
                     /locus_tag="PLO70_14295"
     CDS             <5114..6379
                     /locus_tag="PLO70_14295"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQZ75689.1"
                     /translation="IAILGILLMNIPGFALPSPAYGDPTLMNEIGTINFKTWYFVDWF
                     LEGSQRALFSMLFGAGMILFISRLENKTDGLWPADYFFRRQLWLLAFGLFNAFVLLWF
                     WDVLFHYACCGMILFAFRRLPVKKLLIAAAVCLVLQTARDNVDFYRDKKVITRGEMIA
                     KMDTTQTKLTDIQKDQLQAMTGFKEKTSPEGKKKSMQKSLDKVRGDYAGFYEYQSERS
                     YNSEVKYLYFGLWDILIFMFLGMAFYKSGILTGQAPVKTYWFLFIAGLGGGLVLSWLR
                     LKPMIDYNFNFFEYTKNVKFEFYQISRTFRAVGIFGLIMLLYKSGWCKWFFNLMRPVG
                     QMAFTNYLMQSLLVGLFFYGIGFGYFGKLERHQIYYVVGATWLLQIIWSHIWLRFFRF
                     GPLEWAWRSLTYWKKQPMRKANTKDVMSI"
     gene            6507..>7541
                     /locus_tag="PLO70_14300"
     CDS             6507..>7541
                     /locus_tag="PLO70_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQZ75690.1"
                     /translation="MKRSLITLISLVLVYNLSAQPNWSPEQCLKIKNITAVVPSPDGM
                     KVLYTVREAMMTDDRSEYVNQVWLCNADGSNAIQLTKGDKNSANPKWSHDGKWIAFTS
                     SRDSKNNLYILPVGGGEAEKVTDVKTGVGNYQWSDDGKMIAFTMPDAAETNEEKDKKA
                     KNDWYFMDEQVKQNRLYVLWLEKKDTAGKYVQKKITKENYNVNAFDWSPGGKSIAYSH
                     GKSPEVNDNVYSDISLIDIESGTIKSIANSKAGETNPQFSPDGNHIVYYCTADPVDWS
                     GPRHAKIYSLSDGKAWRLKATPDENGGIIGWTPDGKNVLWNEANRTLSSVYILSADGK
                     SISEWNKGSKD"
BASE COUNT         2259 a         1366 c         1743 g         2073 t
ORIGIN      
        1 aggagagccg gaatattttc agggctgaaa attcgaatac gatctattat ttgtcggata
       61 attgatttga ctaaggagag aaattatagc gtcaccctga gtagtttatc ctgagtgcaa
      121 cgaagggaag ggagactaac gaaattctaa cccggcttcg gcaagctcag cctgacaatc
      181 atcttgtcac cctgagttta tcgaagggtg tttaacgaaa tctttatcag agtgcaacga
      241 aggaaagggt gaagtacgaa atcaaaatgt caccctgagt agtttactct gagtgtaacg
      301 aagggaaggg tgataaacga aattctaacc cggcttcggc aagctcagcc tgacaatcat
      361 cttgtcaccc tgagcttgcc gaaggaggac gaacaaaacg aaggaaaggg tgattaccga
      421 aacgatatta aaaagaaagg aatacattat gccagacagg aaacaggcac ccgcaattgt
      481 cgatgccgta aattttaaac tgaaccttaa accatatcag cattttgttt tggataacgg
      541 ggtggaagtg tatgcagtag atgcaggtgc agaagaagtg atgctgacag aatgggtatt
      601 ctttgccggt aacacccagg aagaacaaaa cctggtagca gccaccacca attttttatt
      661 acgcaacgga accacaaaaa gaactgcttt ccagatcaat gagcattttg aatattacgg
      721 tgcttatctc aaccgtagtt gctataatga aacagctact atcacactgc attgcctcac
      781 caaacatatt gatgagttat taccggtggt aaaagaactg atcacagggt ctaccatgcc
      841 ttctgaggag ctcgctattt accagcagaa tatgaagcag cggttgaaag tgaatctgaa
      901 gaaaagtgat tttgtggctg gccgtttgat tgatgcctat ttatatggag agaaacatcc
      961 ctatggtaaa tacagcagtg ccaaagaatt tgatgtcctg gaaagagacc agttgttagg
     1021 tttttatagc cagtattacc agcagggaaa gctcatgcta tttgtggccg gtaaactgcc
     1081 tgctcacctg gataccctga tgaaccgttt ttttggcgac cttcccaata accccgttga
     1141 agtaaagcac cctgctttgc acccggctac agataagaag attagaatta ccaatgatgc
     1201 agccggtgtt cagggctcta tccgcatagc caggccattt cccaatcgcc atcatcctga
     1261 ttttttaaaa gtacaggtat tgaatgcatt gtttgggggc ttctttggtt cacggctgat
     1321 gagtaatatc cgggaagaca aaggctatac ctatggcata cacagttacc tgcaaaatca
     1381 tattcagcat tcggcctgga tggtaagtac cgaagccggc aaagatgtgt gtgatgcagc
     1441 catcacagaa gtgtataaag aaatgaaaga tctccgcgaa gaactggtgg aggaggaaga
     1501 attattatta gtacgcaatt acatgatggg aggcattctt ggggatcttg atgggccgtt
     1561 ccagatcatt gcccgctgga agaacataat actgaatggg ctgacggagc aatacttcta
     1621 cgattctatc aatactatta aaacgatctc agccgaagag ctgcagggcc ttgcacaaaa
     1681 atacctgcaa cctgaagatt tttatgagtt agtggtggtg taaataattg tatcagcgtt
     1741 tcatgaatga agaaagggat acctctcctg atttactaat tgtttttacc ctgtacaatc
     1801 ctgttggaaa attactgatg tctattttgt ttccggggcc attttgctga agcatcattt
     1861 tcccgtcgct tgtaaaaatc atgattttgt tgagcaggct attattgtca gtggtaatat
     1921 atatatactg acttgccggg cttggaaata cagtaattcc aggcctgttg ccttcagcaa
     1981 atcgtaaagc aataactgaa gaatattgaa accgggcatc caaatcaatt tctttgatgc
     2041 gatagaataa tactcctttg gtaggtgtat tgtccgtaaa cgcataatta ccatcagagg
     2101 agagagtgtt tatgaaaccg gcagtaacaa aattaattcc atcaatgctg cgttcaatct
     2161 catacttgct tacgtttatt tcattctctg atttccattt tagtgaaatt attccttcag
     2221 caattttttg cccgctaaag tgagtgaatg taactggtaa agtgctgcct gtccagttta
     2281 gatttccaat agaaaagctt cttccccctg cggcgcctgc agggttattt ccatattgaa
     2341 tagttaatga acttgtgcag cctgggatgg aaatttgtac cctgtcatca gtttgattgc
     2401 cttgtgtccc tgttccggta gctgtagctg taccgcttcc ggttattgtt ggaggacctc
     2461 ccggaaaacc gggatcaagg gcagtcattg taattgtttg gggagaacct aaatagttgg
     2521 catttaaaat agctctgtct ccacttgtat tatttccatc tatatcataa agaataaaag
     2581 aaagaccgca aaccgcaggg ttgaatgtta aagtataaac ctttaaatca gaaacagcgg
     2641 caaatgtaat tgccgtacgc caggtaatat caccctgtgc ggtggtaaac ctggtaggtc
     2701 ctgcggtaat tgtgcctggc ccggttactg cataagtaac agcagaggcg gggcttccaa
     2761 tagatccaaa attgaaagac aaagaattat ttgggatagc tgatgtgttc cagtttaaat
     2821 cttgcgcctc cagagaacaa gaaaaaaaag aagttatgaa caaagcaaag aacaggtttt
     2881 tcatacacat aagtacataa ttaatagtaa gtaaaaatac ttagattcaa ggaaagccct
     2941 aattttccat cgtcttatat ttatccacac atgtgaaccg gagaatttag atgatggaat
     3001 gaaaacaggt tatgattctg gttcatgtta attaggtttg caaaatgact ttcgaccgta
     3061 aagatttatc cgacgaacag cttgtacatt tctaccgcag tatcctctgg ccccgcatga
     3121 tcgaagaaaa aatgctggtc ttgctgcgac agggaaaaat ttctaaatgg tttagtggta
     3181 tcggacaaga ggccatagct gttggtgcca cactggcctt gcaggaagat gagtggatca
     3241 tgccgctaca ccggaatctc ggcgttttca ctacccgtaa catgccattg agcaaactct
     3301 ttatgcagtg gcagggtgca caggaaggat acagcaaggg aagggaaaga agttttcact
     3361 ttggtaacag ggagcatcat atctgcggaa tgatatcgca tttgggtcca cagctggcta
     3421 ttgcagatgg agtggcatta gcgtataaac ttcgtcatgg ttcgactggg tcaccattaa
     3481 aagtatccct tgcttttacc ggtgatggcg gtaccagtga aggagatttt catgaagcac
     3541 tgaatacagc tgctgtttgg gacctgcctg tcatcttcat cattgagaat aatggatatg
     3601 gattaagcac accaacgaat gagcagtacc gttgcatcag cttaatagat aaagccaaag
     3661 gttatggtat ggagggagtg cagatcgacg ggaataatat attatcggtg tatgatacaa
     3721 taaaaggtgt aagggattac tgtatcaaaa accaaaagcc ctatttaata gaatgtttga
     3781 cattcagaat gagaggacat gaagaagcca gtggtaccag gtatgtgcca caggatttat
     3841 tcaaattatg ggagatgaaa gatcctatta aaaactttga agaattcctg gttggtgcag
     3901 gtgtgttgag cgaaatgaaa gtggcagcaa taagaaatga attcaaggag aagatagaaa
     3961 gtgaattggc gaccgggtcc actataaaac cggtggtagc ggatacggaa gttgaactgg
     4021 aagatgttta cgcagagaag ttcgatgttc ggggtttgaa gtttgatgtt aaaataattg
     4081 acaactcgaa aataaatcat gaattagaag tacaaacgaa agactcccgg ctaatcgact
     4141 ctattaaaga aggtcttcat caatccatgc agcaacatcc taaccttata ttaatggggc
     4201 aggatattgc tgagtacggc ggtgcattta aaataacaga aggatttgta aatgagtttg
     4261 gaaaagaaag agtgagaaat acaccgttat gtgaaagtgc aattgttggt gctgcattag
     4321 gattatcgct ggaagggtac aagagtatga tggaaatgca gtttgctgat tttgtaacgg
     4381 tagggtttaa ccagatcatt aataacctgg caaaaattca ttaccgctgg ggacaacatg
     4441 cagatgtggt gatacggatg ccgacaggtg gtggtgtagg tgccggtcca tttcattcac
     4501 aaagtaatga ggcctggttt gtacataccc ccggattgaa agttgtttat ccttctacac
     4561 ccatggatgc aaagggatta ttgattgcag ctatcaatga tccgaacccc gtgctgttct
     4621 ttgaacataa agccttatac cgttcagtga gcggtcccgt accatcagag tattatgaaa
     4681 ttgaaatagg taaagcaaga catgtaagag aaggcgaaga agtttctatc atcacctatg
     4741 gtgccggcgt actttgggcg gacgattatg cggctgaaca tcctgagatc tctattgata
     4801 tacttgatct ccggactttg ttgcccctgg attacttcgc tatccgtgaa gcagtacaaa
     4861 gaaccggccg tgtattatta ttgcatgagg atacattaac gggcggtatc ggcggtgaga
     4921 tcgctgcctg gatcgctgag aactgttttg gtttattgga tgccccggtg atgcgttgtg
     4981 cttctttaga tacacccatt ccattcaata ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnattgcca tcctcggcat tttattaatg aacatcccgg gctttgcact
     5161 accctcaccg gcgtacggag accccacact gatgaacgaa atcggtacta tcaactttaa
     5221 aacatggtat tttgttgact ggttcctgga aggcagtcag cgggccttgt tctctatgct
     5281 atttggtgcc gggatgatct tatttatcag tcgcctggaa aataaaacag acggattgtg
     5341 gccagccgat tatttcttcc gccgccagtt atggttattg gcatttggct tattcaatgc
     5401 gtttgttttg ttgtggttct gggatgtatt attccattat gcctgctgcg gtatgatctt
     5461 atttgcattt cgccggctgc cggttaaaaa attacttatt gcagcagctg tttgcctggt
     5521 attacaaacg gcaagggaca atgtggattt ttaccgcgat aaaaaagtaa taacccgggg
     5581 tgaaatgatt gcaaagatgg ataccacaca aacaaaactt accgatattc aaaaggacca
     5641 gttacaggcg atgacaggat ttaaagaaaa aacatcaccg gagggaaaga aaaaatcaat
     5701 gcaaaaaagc ctggataaag tacggggtga ttatgccggc ttttatgaat accagagtga
     5761 gcggagctat aattctgaag tgaagtatct ctatttcggt ttgtgggata ttctcatatt
     5821 catgttcctg ggaatggcct tttataaatc aggaatatta accgggcagg ctcctgtaaa
     5881 aacttactgg tttttattta ttgcaggatt aggaggtgga ttggtattgt cctggttacg
     5941 gttgaaaccg atgattgatt ataatttcaa ttttttcgag tatacaaaaa atgtaaagtt
     6001 tgaattttat caaatttcaa gaaccttccg ggctgttggt atttttggat tgatcatgtt
     6061 attgtataaa tcaggttggt gtaaatggtt cttcaacctg atgcggccgg tggggcaaat
     6121 ggcctttacc aactacctga tgcaatcctt actggttggt ttattcttct atggaattgg
     6181 attcggatat tttggcaagc tggaacgtca ccagatctat tatgtagtgg gagctacctg
     6241 gctgcttcaa attatctgga gccatatttg gttacggttt ttccgatttg gtccgctgga
     6301 atgggcctgg cgcagcctga cctattggaa aaaacaaccg atgcggaaag caaatacgaa
     6361 agacgttatg tcaatataaa aggtaaagcg attttttggg atcgttcaaa atgaaaggtc
     6421 aacatctaga taccatgaga atgacttaaa ttaggtttta taatactact atataattta
     6481 atatgtctcc atttaaaatt gcttttatga aaagatcatt aattaccctc atttctttag
     6541 tactggtata taacttatct gctcaaccga actggagccc cgaacaatgt ttgaaaatta
     6601 aaaatataac agcagttgtc ccttcgccgg atggaatgaa agtactctat acagtccgtg
     6661 aggccatgat gaccgatgac cgcagtgagt atgtaaacca ggtctggtta tgcaatgcag
     6721 atggcagtaa tgcaatacaa ctaaccaaag gcgataagaa ctctgccaat cctaaatgga
     6781 gccatgatgg taaatggatc gcctttactt cttcccgcga cagcaagaat aatttatata
     6841 tcctgccggt cggtggcggg gaggcggaaa aggtcactga tgtaaagaca ggtgtcggaa
     6901 attatcaatg gagtgatgat ggaaagatga tcgcctttac catgccggat gcggcggaaa
     6961 caaacgaaga gaaggataaa aaggcaaaga acgactggta ttttatggat gaacaagtaa
     7021 agcaaaaccg gttgtatgtt ttatggctgg aaaaaaaaga tactgccggt aaatatgtac
     7081 agaaaaagat cactaaagaa aattataatg tcaatgcgtt tgactggagt cccggcggaa
     7141 agagcattgc ttatagccat ggtaaatcac ccgaagtaaa tgataatgtg tacagcgata
     7201 tttcgttgat agatattgaa agtggcacca tcaaatcaat tgcaaactca aaggccggtg
     7261 aaacaaatcc gcaattcagt cccgacggga accatattgt ttactattgt actgcagatc
     7321 cggttgattg gtcaggacca cgtcatgcca aaatatattc attgtcagat ggtaaagcat
     7381 ggagactaaa agctacacct gatgagaatg gtggtatcat cggttggaca ccggatggta
     7441 aaaatgtttt gtggaatgag gctaaccgca ccctctccag tgtttatatt ttaagtgcag
     7501 atggaaaatc aatttcggaa tggaataaag gatcgaaaga t
//
LOCUS       DAORTI010000148         7479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11134_length_7479_cov_5.846983, whole genome shotgun sequence.
ACCESSION   DAORTI010000148 DAORTI010000000
VERSION     DAORTI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11134_length_7479_cov_5.846983"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="tilS"
                     /locus_tag="PLO70_14305"
     CDS             <1..489
                     /gene="tilS"
                     /locus_tag="PLO70_14305"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQZ75691.1"
                     /translation="LIKLAASESGRYIESPVLQYRIIKHRHWFIISPAQSISAAHIVI
                     EKTDRSVGFEKGILRLEMTSNQHPVTDNEVACIDAKEISFPLLLRQWKTGDYFYPLGM
                     KKKKKLSRFFIDQKLSKTEKENTWVIESNKKIIWVVGLRVDDRFKVTAKTKQVLRVSL
                     KT"
     gene            complement(478..1131)
                     /locus_tag="PLO70_14310"
     CDS             complement(478..1131)
                     /locus_tag="PLO70_14310"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQZ75692.1"
                     /translation="MELSITLIIIIITVIISFSAFSNQKIIDDLIFYPPAVTKQNQWY
                     RFITCGLIHADVPHLIFNMFSLYMFGEFVERSFASPILFAEKGKFMYLGMYIIALFVC
                     LIPTFIRHRNDYYYRSLGASGAVSAVVFAGILFDPTAKLGFFFIPPIIPGYIFGPVYL
                     IASTYLEKQAKDNVNHSAHIWGALFGIGFIIAAAAILKTDYQPAEQFVWKIRNSLGF"
     gene            complement(1161..1634)
                     /locus_tag="PLO70_14315"
     CDS             complement(1161..1634)
                     /locus_tag="PLO70_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQZ75693.1"
                     /translation="MKISFWSIGKANETYVKEGVEEFTRRISRYYKVEWSIIPVPKNA
                     GMLSEMDLKKKEGEIILEWLNKDDYLVALDERGKQLSSEELAGFIQARANESIKHIVF
                     LIGGAFGIDERVLKRANYKWSLSQLVFPHQLVRLILAEQVYRACTILKNEKYHHK"
     gene            complement(1691..2590)
                     /locus_tag="PLO70_14320"
     CDS             complement(1691..2590)
                     /locus_tag="PLO70_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75694.1"
                     /translation="MKKLNFLILLSVFGLTAGAQNSSFLLYSFKGNVSVVENNAESKA
                     KVGKTMNSTATIKVGPSSAVTLICNEVAMFTLTKAGTYRLNQFGDSCKVSSNSVSANY
                     VKYVWAQMTKSGGSPGSNRKAYMNNVGAVTRGINNIWVDPRLDTVNYSGGEFPLSWKS
                     YADAKQFDFFLYNSENIAEPFYATTVSKLKISIPAFAARMKTGETYYWISAVKGEENE
                     ERKVLNYVSKEVFSALLNDILKQGAQFEGPAEQAYRTAFMLEDAHYLSEAYQYYTKAA
                     TLDPANALYRSTLMSFKKDYEIK"
     gene            complement(2619..3863)
                     /locus_tag="PLO70_14325"
     CDS             complement(2619..3863)
                     /locus_tag="PLO70_14325"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQZ75695.1"
                     /translation="MKKPPHGQNGTGAKRFGTHFTKYLLERDTVFATIWVFIFIVGLG
                     SIPLNLYVLNPLKMGLKDFDYTDLAYAELGKSADSLDKNIVIINIGHADRELLSMIID
                     KTAENNPKVIALDALFDEAKDPYQDSLLSASFGKHKNFIAATKLNLSGEDGDTITITG
                     DYFKNAATYGYANFFSDELASLRYYDPFLKDFKDNRYNSFTSAIIQAYDPTAFKMIKN
                     KEGKQVQINYERRVDKYQVIEYDNLLTGSFERKAIEGKIALLGYINRDPTDILDKKFT
                     PMNERFAGKSRPDMNGIVVHANIISMVLGNNYIKKVPSWVNLLIAVVVCWLFMSFFIR
                     YYLENHIWFHLVAKIIQVAAVLLFAYLGIYFYDAYSIKLDMKLSLVVIIMAVDVIYFY
                     EAWAVWMHKKFHYDTIFKPHHH"
     gene            4039..7368
                     /locus_tag="PLO70_14330"
     CDS             4039..7368
                     /locus_tag="PLO70_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ75696.1"
                     /translation="MRISLSITILGLVILFSGYKAAAQSPPTPRDTSIKGPQTFAIIM
                     GISKYKYVRPLSYADKDAEMFRDYLKSPAGGSISTDNIFILLNEQALSTNFWSKGFKW
                     LDAKKLQKGDRLFIYLAGHGDAIDEDQFFYLTYDCNPAGDKNNYIAGGNVQLFNLKKK
                     IAKETAKGAEVFFIMDACRTSELPGGTEGQNFLNTAISEKRAGEIIMLATGAGQESLE
                     DASIGTGHGLFTYYLVDGLNGLADSEGTPDNKVTLAEIQKYVDKNVPAVAQQRFRRKQ
                     DPYFCCNENSDKVVSIVDAEHLKKWMETRKKGSGNSFTGNLRNKRVYTAPADTQLLQT
                     YSLFNEAVKQNKLTGKESAEFYYSLLENKFPGNAYTTDAQSTLAVEFINFAQTKINLY
                     LDCKDAVAIQRIRAQLDDEDKTDEIAASVDRMEKVAQQEFYEVGIMLEKAINIIRQDD
                     PDFAESLTGRMYFFKARGYFGKAKKAVDITKAFEYAYNAFAKDKNAAYILNTISSLHL
                     DNNNYDSAIFYAKKAMITAPRWRYPYVTLAFAYRTLNKPDSAIKYYRKSIEVDPRNAD
                     AYVDLGHYYFSLSKGDSAIAYYEWALAIEPGNIYAGNNIGWLYFNRKKYADAIRYFKS
                     SILTDPKFINAYNGLGKSFFELKEYDSARIYYSKAFANYQDKSIVNVYIGNFYRDLKA
                     YDSAKVYYRLAAALAPDYEEAYNNLGRSSFALKQYDSARLFYRQALQANPYSAFALIN
                     MGLVFKELKETDSTYNYFQKAIWLEPKNPSILNNLGVIYGEDKNYDSAKKYFRRALFV
                     RPNYKPASKNLMLIFKELNQLDSVTNFLKGSSLFDPGSTSFMNDMGLAFLDQKRYDSA
                     RWYIRKAIMLDPVNPQLLNNMGRVLQSMKQYDSARVYIQKAISVDPENAVLLMRLADV
                     FKQQKRPDSAAHYFKRQFAINAVSNSATTYQSIGSFFAEMKMPDSAVIYYKKAISADP
                     AYITAYINAGNTYMELEENDSALVYLRHAVRINPDNFIAVYNLGLVYHVMNIYDSAIV
                     YIQQAIRMEPARGETYYQLACTYALHNKPEQAILYLRQAYERGYKNKESLLTDPDLFE
                     LKNYKEFQALLDKYIPGWKER"
BASE COUNT         2234 a         1491 c         1560 g         2194 t
ORIGIN      
        1 ctgatcaaac tggccgccag tgaatctggc cggtatattg agtcgcctgt tttgcaatac
       61 cgcattatta aacaccggca ctggtttata atcagtccgg cacaaagtat ttctgctgct
      121 catattgtaa ttgaaaaaac ggatagatct gttggttttg aaaaaggaat actccggctt
      181 gaaatgacca gcaaccagca cccggtaacc gacaacgagg tcgcctgtat cgatgccaaa
      241 gaaatttctt ttcctctttt actccgccaa tggaaaaccg gcgactattt ctacccgctg
      301 ggaatgaaaa aaaagaaaaa gctatcccgt tttttcattg accagaaatt atccaaaacc
      361 gaaaaagaaa atacctgggt gatagagagc aataaaaaga tcatttgggt ggttggcctc
      421 cgggtagatg atcggtttaa ggtaacagcg aaaacaaagc aggtgctccg ggtttcttta
      481 aaaacctaag ctatttctta ttttccacac aaactgttct gccggctgat aatcggtttt
      541 taaaatcgct gctgccgcaa taatgaaacc gatgccgaat aatgctcccc agatatgggc
      601 agagtggttc acattgtctt ttgcctgctt ttcaagatac gtggaagcga tcaggtacac
      661 cggtccgaat atatacccgg gaatgatcgg gggaataaaa aagaaaccca atttagccgt
      721 aggatcaaat aatataccgg caaataccac cgcactgact gcaccggaag caccaagact
      781 gcggtaatag taatcattcc tgtgcctgat gaaagttggg ataagacaaa cgaacaaggc
      841 aattatatac ataccgaggt acatgaattt tcctttttct gcaaagagga tcgggctggc
      901 aaaactccgc tctacaaatt ctccaaacat gtatagggag aacatattaa aaatgaggtg
      961 aggaacatca gcatgtatca gtccgcaggt gataaaacga taccattgat tttgtttggt
     1021 gacggcaggg ggataaaaga tcaggtcatc aatgattttt tgattactga aggcactgaa
     1081 agaaataata accgtaatga tgataatgat aagtgtgatt gataattcca tgctgttaga
     1141 ttcagggctg aaaataaaaa ttatttatga tgatactttt cgttttttaa aatggtgcag
     1201 gccctgtata cctgttcggc cagtatcagc ctgacaagct ggtggggaaa aaccagttgg
     1261 gacaggctcc atttataatt ggctcttttc aataccctct catcaatacc aaaagcacca
     1321 ccaatcagga aaacaatatg ctttatactt tcattggccc ttgcctggat aaaaccggcc
     1381 agttcttccg agctcagttg ttttcctctt tcatccagtg ctacgaggta gtcgtcttta
     1441 ttcagccatt ccagtattat ttcaccttct ttttttttca ggtccatctc actcagcatg
     1501 cccgcatttt taggtaccgg gataatgctc cattccacct tataatatct tgagatgcgg
     1561 cgggtgaatt cttccacacc ttcctttaca tatgtttcat tggctttccc gattgaccag
     1621 aatgatatct tcattggtta aaaatagaaa agtcctgcca tagcaggact tttttgccgg
     1681 gttaatcggt ttattttatt tcatagtctt ttttgaaaga catgagtgtg cttcggtaca
     1741 aagcattcgc cggatcaagc gttgccgctt ttgtgtagta ctggtacgct tcagaaagat
     1801 agtgtgcatc ttccagcatg aacgctgtac gataggcctg ctctgccggc ccttcaaact
     1861 gtgctccctg tttgagaata tcattcagca atgcagaaaa aacttctttt gaaacataat
     1921 tcaatacttt tctttcctca ttttcttctc ctttaacagc tgaaatccag taatacgttt
     1981 cgccggtttt catccgggcg gcgaatgccg ggatagaaat ttttaacttg ctgacagtgg
     2041 tagcataaaa aggctcagcg atgttctcac tattatacag gaaaaagtca aactgttttg
     2101 catcagcata tgatttccat gacaatggaa attctccacc ggaatagtta acagtatcca
     2161 gccggggatc tacccagata ttattgatgc cacgggttac tgcacccaca ttgttcatat
     2221 aggctttacg attactgccc ggagatccgc cggatttggt catttgcgcc catacatatt
     2281 ttacataatt ggcacttact gagttgctgc ttactttaca ggaatcacca aactggttaa
     2341 gccggtaggt acctgcttta gtaagggtaa acattgccac ttcattacag ataagcgtaa
     2401 cggcagagga tggccctact ttgatagtgg cagtactgtt catggttttg cccactttgg
     2461 ctttactttc agcattgttt tctacaaccg atacatttcc cttgaaactg taaagcagga
     2521 aactgctgtt ttgcgccccg gccgttagtc cgaatacaga aagtagaata agaaagttca
     2581 gctttttcat tttatatgtt tttttagatc aatcgctttt aatggtggtg cggtttgaaa
     2641 atagtatcat aatgaaattt tttgtgcatc catactgccc aggcttcata aaaatagatg
     2701 acgtctaccg ccataatgat aacgaccagg ctcagtttca tatccagttt aatactatac
     2761 gcatcgtaaa aataaatccc gagatatgca aaaagcaata ctgcggcaac ctgtattatt
     2821 ttagctacta aatggaacca gatatggttt tcgaggtaat accggataaa aaaggacatg
     2881 aacagccagc aaactaccac cgctattaat aaattcaccc acgaaggtac tttttttata
     2941 tagttgttcc ctaaaaccat ggagatgata ttggcatgta ccacaattcc gttcatgtcg
     3001 ggccgggatt tacccgcaaa ccgttcattc ataggagtga atttcttatc cagaatatct
     3061 gtcgggtccc tgttgatata acccagcagg gcaattttac cttcaattgc ctttcgttca
     3121 aaactcccgg tcaataaatt atcatactct atcacctggt atttatccac tctacgttca
     3181 taatttattt gcacttgctt gccttctttg tttttgatca ttttaaaggc tgttggatca
     3241 taagcctgga taatagcact ggtaaaggaa ttgtagcgat tatctttaaa gtctttcagg
     3301 aatggatcat aatagcgcag tgaggcaagc tcatcactaa aaaaattggc gtacccgtat
     3361 gtggctgcat ttttaaaata atcaccggtt attgttattg tgtctccatc ctcgccggac
     3421 agattgagtt ttgttgcagc aataaaattt ttatgcttac caaatgatgc agaaagaagg
     3481 gagtcctggt aaggatcttt agcttcatca aaaagagcat ctaacgcaat gactttagga
     3541 ttgttctctg ccgttttatc tatgatcatc gacagaagtt cccggtctgc atggccaata
     3601 ttaatgataa caatgttttt gtcgagtgaa tctgcacttt ttcctaattc cgcatacgct
     3661 aagtcggtat agtcgaagtc ctttaatccc attttcaaag gattcagtac atacagatta
     3721 agaggaatgc tgcccaaccc gacaataaaa atgaagaccc agatggttgc aaaaacagta
     3781 tcccgttcaa gcaggtattt ggtaaaatgt gttccgaatc ttttggcgcc agttccattt
     3841 tgcccgtgtg gtggcttctt catgacattg ctttgtaggt tgttggtggt ttgttttgta
     3901 tgattcctta atacagttca gggttgcagc ttatccgtat tttaacctgg cttgttttat
     3961 agataccttc ttgagggtat ttgtgtcccg gtttaactgc cattaccctg taaatccgta
     4021 atttagaaaa tctaatttat gcgaatttcc ttatccataa cgatacttgg cctggtaatt
     4081 ctcttttcag gctataaagc tgctgcccaa tcaccaccca ctccaaggga taccagcatc
     4141 aaagggccgc aaacttttgc aataattatg gggatatcaa agtacaaata tgtccggccc
     4201 ttatcgtatg cagataagga tgctgaaatg ttcagggatt acctgaaatc gcctgccgga
     4261 ggtagtataa gcacagataa tattttcatt ttgcttaatg aacaggcttt gtccaccaat
     4321 ttctggagta aaggttttaa atggctggat gcaaaaaaac tgcaaaaagg ggaccgatta
     4381 tttatatacc tggccggcca tggtgatgca attgatgaag accagttttt ttacctgact
     4441 tatgactgca atcctgccgg ggataagaat aattatatag caggaggcaa tgtgcagctt
     4501 tttaacctga agaaaaaaat tgcaaaagaa actgccaagg gagcagaagt atttttcatc
     4561 atggatgcct gccgcaccag tgaattgccg ggtggaacag aaggacaaaa ttttttgaat
     4621 accgctattt cagaaaaaag ggccggggag atcatcatgc tggcaacagg agcggggcag
     4681 gaatcactgg aggatgcttc aattggtacc ggccatgggt tatttactta ttacctggtg
     4741 gatgggttga atggtcttgc tgactcagaa ggtaccccgg ataataaagt gacattagcg
     4801 gaaatacaaa agtatgtaga taagaatgtg cctgctgtgg cgcagcagcg tttcaggcga
     4861 aaacaagacc cttatttctg ttgcaatgag aatagcgaca aagtagtaag tattgtagat
     4921 gctgaacacc tgaaaaaatg gatggagaca agaaaaaagg gatctggtaa ttcttttacc
     4981 gggaacctgc ggaataaaag agtgtataca gccccggcag atacgcaatt gctgcaaacg
     5041 tactccctgt ttaatgaggc agtaaaacag aataagctga cgggtaaaga atcagcggaa
     5101 ttttattata gtttgctcga aaataaattc cccggtaatg catacactac ggatgcacaa
     5161 tcaacgttgg ctgttgagtt catcaatttt gcacaaacaa agatcaacct ttacctggac
     5221 tgcaaggatg ctgttgctat acaacggata agggcacaac tggatgatga agataaaaca
     5281 gatgaaattg ctgccagtgt agaccggatg gaaaaagttg cccagcagga gttttatgaa
     5341 gtgggaatta tgctggaaaa ggccattaat attatccggc aggatgatcc tgattttgcc
     5401 gaatctctta ccggccggat gtactttttt aaagccaggg gttattttgg taaagcgaaa
     5461 aaagcagttg acatcaccaa agcttttgaa tatgcgtaca atgcgtttgc caaggataag
     5521 aatgctgctt atatcttaaa tactatttcc tccctgcacc tggacaataa taattatgac
     5581 agtgctattt tctatgccaa gaaagcgatg ataacggctc cccggtggcg atatccatat
     5641 gttacactgg cttttgctta ccggacatta aataaaccgg attctgccat aaagtattat
     5701 cgtaaatcca tagaagtgga cccccgtaat gctgatgctt atgtggatct tggtcactat
     5761 tatttttctc tttcaaaagg ggattcagct attgcttatt atgaatgggc tttagcgata
     5821 gagccgggta atatttatgc gggtaataat attggctggc tttattttaa ccggaaaaaa
     5881 tatgctgatg ccatcaggta tttcaagagc agtatactaa cagatccaaa attcataaat
     5941 gcctacaatg gattgggtaa aagctttttt gaactgaaag aatatgactc ggccaggatc
     6001 tattattcaa aagcatttgc caactaccag gataagtcga ttgtgaatgt gtatatcggg
     6061 aatttttacc gtgaccttaa agcctatgat tctgcaaaag tgtattaccg tttggcagct
     6121 gcacttgctc ctgattatga ggaagcatat aataacctgg gccgttcaag ttttgcctta
     6181 aaacaatatg attctgcccg cctgttttac cggcaggccc tgcaggcaaa tccctattct
     6241 gcatttgcac tgatcaatat gggattggtg ttcaaagaat tgaaggaaac agattctacc
     6301 tataattatt ttcaaaaagc tatttggctt gagcctaaga atccctctat actgaataac
     6361 ctgggtgtta tttatggaga agataaaaat tatgattcgg ccaagaaata tttccggagg
     6421 gccttatttg taagaccgaa ttataaacca gcatccaaaa acctgatgct gattttcaaa
     6481 gaactgaacc aacttgattc agtcacaaat tttctgaaag gctcttccct gtttgatccc
     6541 ggcagtacat catttatgaa tgatatgggt ctggctttcc tggatcagaa aaggtacgat
     6601 tcagcacgat ggtatatccg aaaagctatt atgctggatc cggtcaaccc gcaattatta
     6661 aataacatgg gaagggtgct acagagtatg aaacaatatg attcagccag ggtctatatc
     6721 cagaaagcca tatcagttga tccggagaac gctgtgctgt taatgcggct ggccgatgtt
     6781 ttcaaacaac agaaacgacc cgattcagca gcccactatt tcaaaaggca atttgctata
     6841 aatgcagttt caaacagtgc gacaacttac cagtcgattg gcagtttctt tgcggagatg
     6901 aaaatgccgg attctgcagt tatatattat aaaaaggcaa tttcggctga tccggcatac
     6961 attaccgctt atatcaatgc aggtaacacc tatatggaac tggaagaaaa tgattccgca
     7021 ttggtttacc tgcgccatgc agttagaatc aacccggata attttatagc ggtatataat
     7081 cttggcctgg tttatcatgt gatgaatatt tatgattctg ctatcgttta tatacagcaa
     7141 gctatccgaa tggaacctgc caggggagaa acttattatc agttggcatg cacttatgct
     7201 ttgcacaata aacccgaaca ggctattttg tacctgcgcc aggcttatga aaggggatat
     7261 aagaataaag aaagcctgct gacagacccg gatctgtttg aattaaagaa ctacaaagaa
     7321 ttccaggcct tactggataa gtatattcct ggctggaaag agcggtaaat gaaaagaggc
     7381 cttacctaaa taaaacagct ttacttaatt ttcctggcaa tcatcacgta tcgtatatct
     7441 actaatataa aacattggga tgaaatttgt tacggcaga
//
LOCUS       DAORTI010000149         7462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11169_length_7462_cov_8.329553, whole genome shotgun sequence.
ACCESSION   DAORTI010000149 DAORTI010000000
VERSION     DAORTI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11169_length_7462_cov_8.329553"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="PLO70_14335"
     CDS             complement(<1..2399)
                     /locus_tag="PLO70_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75697.1"
                     /translation="MKKVYILLLLAISCSIIQGQTWNGSVNTGWDNASNWTPAGLPTN
                     TGNIIIPGGLTNYPVLAGKVTINSIDMQPGSQLDVNGSDLTITGVNMNINFNGAVINN
                     SNGATDIVLNINTGTGGFLTYFRSNTINDNIVFNLTGSNQFIEAVAAPANVYNGNATF
                     NINDVLTTYISYGVPSQFNGNLTVNRTVAGPTSLFYAGASITGNFSYINNTGSSTSMG
                     NLSYKTSIGGTVNITAHYPSPGGFEILRLVNQTTGGTVDVQNSQGFNVQNDTLLVTAL
                     SITGYRGNQYANLMNNSITGTVTTADDASYGNGFLTYIRNNIINGNSSFSNNGSNIFA
                     EANVSGSGNIYNGNVIFNGGGAGNLLISYGAPLQCTGDLTISRTAAGLTSAFNSGAVI
                     GGNFTYTNNTAGETGFGNLANATTIGGTVNITANYTTPNNFGIHRLVNQTNGGSITVT
                     NSLGFNVQNNTLLLSAMNITGYKGGQYGYLFNNAITGNVTIADDISFGEGFSTNIRNN
                     IITGNSNFTITGINTFAEADGPGYGNRYNGNLTLNCAGNGNVYISYLDTLECTGDLTI
                     NRTAAGLTLAFNSGAVIGGNFTYTNNTAGETGFGNLLYKTSIGGTININANFTTPNNF
                     GLHRLVNQTNGGSITVTNSRGFSVQNDTLLLTVMSITGYKGGQYGYLFNNAITGNVTI
                     ADDISFGGGYSTNIRSNIITGNSSFTISGTNSFDEADGAGFGNRYNGNVSFTGAGPAS
                     MFIAYLDTLHCTGNVSINRTAAGLTSAFNAGATINGNFTYTNNTAGETGFGNLANKTS
                     IG"
     gene            3085..4167
                     /locus_tag="PLO70_14340"
     CDS             3085..4167
                     /locus_tag="PLO70_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ75698.1"
                     /translation="MKKRFMAAFLLLSLSYSNSLTAQDNDSLTRIYSKFDFIPGEKVI
                     FFDDFSSENIGDFPVLWNTTGSGEIVTYGKYPGKWLKITNPRSITTLFDPLTLPENYT
                     IEFDVVPQAQPPSHNNSYFNFYLISTIKPKDPLFGLARPGGTGVKLSFAYSQYFSAYY
                     SDGTPDVKSTNGDAAIRQKAETKYHISIWVQKQRIRVYQDEVKIFDLARAISDKVKYN
                     MIRFHEGTPLIGNVRIATGLPDMRNKLITEGKLVSYGIYFDVNSDKLKPQSAGTLKEI
                     AGIMAENPDLKIRIVGHTDADGADAANLDLSKRRAASVKDALSTAYGIEANRIETDGK
                     GEKEPVAPNDNGTNKALNRRVEFIKL"
     gene            4197..4607
                     /locus_tag="PLO70_14345"
     CDS             4197..4607
                     /locus_tag="PLO70_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75699.1"
                     /translation="MKRLLIIPGVFSIFMIIASLTPIKDSKDDNVTSIKTDKVSDITQ
                     VSVKCFFTIAGNNMAKAGVCLNLTGSPTTPSKIDIPLSVPTVILNSARPKQFNVGVKG
                     LTWNTTFYIRAYVKNNDGTVVYGNELKFKTLPKP"
     gene            complement(4751..5452)
                     /locus_tag="PLO70_14350"
     CDS             complement(4751..5452)
                     /locus_tag="PLO70_14350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQZ75700.1"
                     /translation="MKRLLPLLLPFFLVACEKTVNTTVPINNNPPPGNALKTWLALGD
                     SYTIGQGVGDAERFPAQTANWLKQNGININIPYYIATTGWTTVNLQSAINDQNPGTYD
                     VVSLLIGVNDQYQTHDTTGYRQRFTDLLVKAIQLARGKKENVFVLSIPDYSVTPFAAG
                     NDTARIRQEIDWFNAINKSVTNSYNCPYLDITPSTREGRYDRSLIAADGLHPSGLEYR
                     RWAFRLGPMMLPVLR"
     gene            5467..5967
                     /locus_tag="PLO70_14355"
     CDS             5467..5967
                     /locus_tag="PLO70_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75701.1"
                     /translation="MKRENGSEVMVKENCFIDPALSVDCDHWKRADACRKLKMIRKQE
                     GEIMKTLDFFKTMAWLASTTTLGSRGIPGEANYFFDHGAEPFVDIRISMNDLFTNKFM
                     LAGGFDHKKGIPVMTHSQDRFHLHEMKFIKYEFIHTSQKLLRRRRSLKPHTKKSYAYR
                     NHWFKN"
     gene            complement(6022..7050)
                     /locus_tag="PLO70_14360"
     CDS             complement(6022..7050)
                     /locus_tag="PLO70_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75702.1"
                     /translation="MKKIKWLLFVCVIMNSFISGAQTPVDSIRFFTEEGIIEMTLTTD
                     IRKLRAKESIEAYQPADISVRFPGNTIVSEPIRLYARGKSRRETCNLPPIMLDFHNPS
                     SPLLYPLSRLKLVVGCGTSEQDEQLVLKEYILYKMYNLLEERSFRARLAKVTYNDSRG
                     KMKSYSQYAFLLEDVDDMAKRNGCKASKKMSFHVGETDPGLMNKVEIFEYMIGNTDWS
                     VPGNHNIKMIYPRNDEKAPVLPVPYDFDNSGLVNAYYAVPNELLGSTSVTQRIYRGYV
                     RTLPEVQQTLDLFRAKKESLFALVKNFELFKSKTRDEIINYLNDFYKAIESESSVKFI
                     FVDNARRE"
BASE COUNT         2163 a         1530 c         1535 g         2234 t
ORIGIN      
        1 ccgatactgg ttttattggc caggttacca aaccctgttt cacctgcagt gttgttggta
       61 taagtaaagt tgccgttaat ggtggcgccg gcattaaatg ctgaagtaag tcctgctgct
      121 gtgcggttga tgctgacatt gcccgtgcaa tgcaatgtat ctaaatatgc aatgaacata
      181 ctggcagggc cagcacctgt aaaacttaca ttaccattat agcggttacc aaaccccgca
      241 ccatctgctt cgtcaaatga attagtacca ctgatggtaa agctgctgtt gccggtaatt
      301 atattgctgc ggatattggt actataacct ccaccaaagc tgatatcatc ggcaatggtt
      361 acattgcccg taatggcatt attgaataaa taaccatact ggccaccttt gtaacctgta
      421 atactcatta cagtaagtag cagcgtatca ttctgcacac taaagccccg tgaattggtt
      481 actgtaatgc tgcctccatt ggtttgatta accaaacggt gcaaaccaaa attattaggc
      541 gtggtaaagt ttgcgttaat atttatggta cctccgatac tggttttata aagcaggttt
      601 ccaaaaccgg tttcgcctgc tgtattgttg gtataggtaa aatttcctcc tattacagct
      661 cccgaattaa atgctaaagt taatcctgca gctgtgcggt taatggtaag gtctcctgtg
      721 cattccaggg tatccaaata ggatatgtaa acatttccat tgcctgcaca atttaaagta
      781 aggttaccat tataacggtt gccgtaacca ggtccatctg cttctgcaaa agtgttgatg
      841 ccggtaattg taaaattgct gttgccggta attatattgt tgcggatatt ggtactaaaa
      901 ccttcaccaa agctgatatc atcggcaatg gttacattgc ctgtaatggc attgttgaat
      961 aaataaccat actgaccacc tttgtaacct gtaatattca ttgcggagag cagcagggta
     1021 ttattttgta cattaaagcc cagcgaattg gttacagtaa tgctgccgcc attggtttga
     1081 ttaaccaaac ggtgtattcc aaaattattg ggtgttgtat aattggcagt tatgtttaca
     1141 gtaccgccaa tagttgttgc gttagccagg ttaccaaacc ctgtttcacc tgcagtgttg
     1201 ttggtatagg taaaatttcc tcctattaca gctcccgaat taaatgctga ggttagtcca
     1261 gctgctgtgc ggctaatggt aaggtctcct gtacattgca gtggtgcgcc gtaactgatc
     1321 aataaattac cggctccgcc cccgttaaat attacattac cattataaat atttcctgaa
     1381 ccgctaacat tagcttctgc aaatatattg ctgccattat tggaaaaact gctattgccg
     1441 tttattatat tgttgcggat ataggtgaga aaaccatttc cataactggc atcatcagca
     1501 gttgttacgg ttcctgtaat actattgttc attaaattag catactggtt gcccctgtaa
     1561 ccagtaatgc ttaaagcagt aaccagtaat gtatcatttt gcacattgaa accctgagaa
     1621 ttttgaacat caactgtacc tcccgtggtt tggttaacca aacgcagtat ttcaaatccg
     1681 ccgggcgatg gataatgcgc agtaatattt actgtacctc caatacttgt tttataagaa
     1741 agattgccca tgcttgtaga tgaaccggta ttattgatat aactgaaatt gcctgtaatg
     1801 gaggcccctg cataaaacag gctggtgggt ccggcaactg ttcggtttac agtaagattg
     1861 ccattaaact gtgagggtac cccataagaa atataagtag tgagcacatc attaatattg
     1921 aaagttgcat tgccattata cacatttgca ggtgctgcaa ccgcttcaat aaactggttg
     1981 ctgccggtaa ggttaaatac aatattatca ttgatggtat tacttctgaa atacgtaaga
     2041 aaaccacctg tgccggtgtt gatattcaaa acaatatcag tagcgccatt actattattg
     2101 ataactgcac cgttaaaatt gatgttcata ttaacaccgg taatggtaag gtcggagcca
     2161 tttacatcca gttgactgcc gggttgcata tcaatactgt tgatagttac tttacctgcc
     2221 agaaccgggt aattggtcaa tccgcccggg ataattatat tacctgtatt agtgggtaag
     2281 ccggcaggag tccagttgga tgcgttatcc caacccgtat ttacagagcc gttccatgtt
     2341 tgtccctgaa taatgctgca ggagattgca agcagcaata aaatgtaaac ctttttcata
     2401 aaataaaaat taaagtgaac tgtaacatca atcactctct tcaataaagg ttaagacccc
     2461 tgcgccctga ttaaacaaac tgctccctcc aaagggagaa ccttgtttaa aatgctgatt
     2521 cgagtgcaat tttattttgc acagcttaaa ccttctttgt tgtgtcttgt gtggattttc
     2581 cgtagtgtgg tctgacggta agcgccggtc tgcaatcccc aggccggtgt atcaacgctt
     2641 atccatggat ggaagatgtg ttactaagtt acgataccag tgtggcggtt caaaaaattt
     2701 tcaggtgatt cttatcagct acgcatcagt tctatcaatg aatcttaatt aaacttgcta
     2761 tacagtaaca ataaaatata gtttaatgcc gggtaatgaa tctgctcttt actctatgcc
     2821 attatatctt gttggtaaaa ttgaagccgg tcaggatata caggataata tatatagttg
     2881 ttttcttacc ggactgtatt ataaaactct tttcttacca catgacaaga atcatacttc
     2941 tttctgttat gtataattgc ttctcctgta tatcctgttc tacctttagt acacataatt
     3001 aagtaaagct ctttcgttgc cgctctttct atgctgaaac gaatgggctt tcagcactat
     3061 tcgtacacat caaattacca gcatatgaaa aaaagattca tggctgcatt cctgctacta
     3121 tcattatcat acagcaacag cctgacagcc caggacaatg attcgttgac ccgtatttat
     3181 tcaaaatttg attttatacc gggagagaag gttatttttt ttgatgattt ttcttctgaa
     3241 aacatcggcg actttcctgt tttatggaat accaccggtt ccggggaaat tgtgacctat
     3301 ggtaagtatc ccggcaagtg gctcaagatc accaatcccc gcagcattac aaccctgttt
     3361 gatccattga cactgccgga aaattatacc attgagtttg atgtggtacc gcaggctcaa
     3421 ccaccttctc acaacaatag ttatttcaat ttctatctta tcagtaccat caagccaaaa
     3481 gacccgttat tcggcctggc caggccggga ggtaccggcg ttaaactgtc ctttgcttac
     3541 agccaatact tctctgcata ttattcggat ggtacacctg atgtgaagag caccaatggt
     3601 gatgctgcca tcaggcaaaa ggccgaaact aaatatcata tctccatctg ggtacaaaaa
     3661 cagcgaatcc gggtgtacca ggatgaagtc aagatctttg atctggcccg tgccatatct
     3721 gataaagtga agtataacat gattcgtttt catgaaggca ccccgttgat cggtaatgtc
     3781 cgcattgcca ccgggctacc tgatatgcgc aataaactca tcactgaagg taaactggtg
     3841 agttatggta tttatttcga cgtgaattct gataagctga aacctcagtc ggcaggtacg
     3901 ctgaaagaaa tagcaggcat catggctgaa aaccctgacc tgaagatcag gattgttggt
     3961 cacacagatg ccgatggtgc cgatgctgcc aatcttgatc tgtccaaacg ccgggcagca
     4021 tctgtaaagg atgctttgag cacagcttac gggattgagg caaaccggat tgaaaccgat
     4081 ggcaaaggag agaaagaacc tgttgctccc aatgataatg gcaccaacaa agcactgaac
     4141 cggagagtgg agttcattaa actgtaaaca agttttttca ccaaaaaccc aagcagatga
     4201 aaaggctatt aataataccg ggtgtgttca gcatctttat gatcatagcg tcattaacac
     4261 ccataaaaga ttcaaaagac gataatgtta cctcgataaa aacggataaa gtcagtgata
     4321 tcacccaggt aagcgtaaag tgttttttca ctattgccgg caataatatg gctaaggccg
     4381 gggtttgtct gaatctgacc gggagtccga ccactccttc caagattgac attcctttat
     4441 cagtaccaac tgttatcctt aattcagcaa gacccaaaca gtttaatgta ggggtaaagg
     4501 gccttacctg gaataccact ttttatataa gagcctatgt aaaaaataat gacgggacgg
     4561 tggtttacgg aaatgaatta aaatttaaaa cattaccaaa gccgtagaaa aaggccggat
     4621 tttattaaga ttttattttt aggatttgag tcaaaaagca tcaaaaaaag ccattttatg
     4681 gctttttttg atgaaaaaga cctcaaaaag gacaattttt atcaaattca aataattcta
     4741 aaactaaagt ttagcgcaat acgggaagca tcatcggtcc tagtctgaaa gcccagcgac
     4801 ggtattccaa gccggaagga tgaaggccgt cggcagcgat caggctgcgg tcatacctgc
     4861 cttcccgggt ggaaggcgtg atatcgagat aagggcaatt gtaactattg gtcacacttt
     4921 tattaatggc attgaaccag tcaatttcct gccggatgcg ggccgtatca tttcctgccg
     4981 caaacggggt gacactataa tcaggtatcg atagcacaaa tacattttcc tttttccccc
     5041 ttgccagctg gattgctttt accagcagat cggtaaaccg ctggcggtaa cctgttgtat
     5101 catgggtctg gtattgatca ttcaccccga tcagcaggct cactacatca taagtgccgg
     5161 gattctgatc attgatagcg gattgcaggt tgacagtggt ccagccggtg gtggcgatat
     5221 agtacggaat attgatatta atcccgtttt gctttaacca attggcggtt tgggcaggaa
     5281 atcgttctgc atcacccaca ccctgcccga tggtatagga atcgccaagg gccagccatg
     5341 tcttcaatgc attcccgggc ggcgggttat tattgatggg cacggtggta ttgaccgttt
     5401 tttcgcaggc aactaaaaag aagggaagca gtaaaggcaa caaccttttc atatttcaaa
     5461 ttacgaatga aaagggaaaa tggttcagaa gtgatggtaa aagaaaactg ctttattgat
     5521 ccggcactat cagttgactg cgatcattgg aaaagagctg atgcctgtcg taaattaaaa
     5581 atgatccgga agcaggaagg agagattatg aaaacgcttg atttttttaa aacgatggcc
     5641 tggttggcaa gtaccaccac attaggcagc aggggtattc ccggggaagc aaattatttt
     5701 tttgatcatg gtgctgaacc ttttgttgat atacggataa gcatgaatga tttattcacg
     5761 aataagttca tgcttgccgg tggttttgat cataagaagg gaatccctgt tatgacacat
     5821 tcccaggatc ggtttcatct tcacgaaatg aaattcatta agtatgaatt tattcatact
     5881 tctcaaaaat tgttgcggcg aagaaggagt ttaaaaccac ataccaaaaa atcttatgct
     5941 tatagaaacc actggtttaa aaattaagga ctctttcttt tcctggttgc actggcctta
     6001 caacaggcta agaaaatagt cttactctct ccttgcatta tctacaaaaa tgaacttcac
     6061 actgctttca ctttcgatgg ctttataaaa atcattcagg taattaatta tttcgtctct
     6121 tgttttggat ttgaatagtt caaaattctt caccagggcg aagaggcttt cctttttagc
     6181 cctgaaaaga tccagtgttt gctgtacctc gggcagggtt cgtacatatc ccctgtagat
     6241 tctttgtgta acggaagtac tgcctaataa ttcattggga acagcgtagt acgcatttac
     6301 aagtcctgaa ttatcaaaat catacggaac aggaagtacc ggggcttttt catcattcct
     6361 ggggtatatc attttaatat tatgattacc cggaaccgac cagtctgtat tcccaatcat
     6421 atattcaaag atctctacct tattcatcag tccgggatct gtttctccaa catggaacga
     6481 cattttttta ctggctttac agccatttct ttttgccata tcatctacat cttctaacaa
     6541 aaatgcatat tgggaataag atttcatctt gccccggctg tcattataag ttactttggc
     6601 cagtcttgca cggaaacttc tttcctctaa aagattatac atcttataaa ggatgtactc
     6661 tttcagcacc agttgttcgt cctgttcgga agtgccgcag ccaactacca gttttagtct
     6721 gcttaacggg tataatagcg gagagctggg attatgaaag tccagcataa tgggaggcag
     6781 gttacaggtt tcccgtctcg actttccccg ggcataaagc cggatgggtt cactgacaat
     6841 agtattacca ggaaaacgaa ccgaaatatc agcaggctgg tacgcttcta tactttcttt
     6901 tgcccggagt tttctgatat ctgtggtaag ggtcatttca atgatccctt cttcagtaaa
     6961 gaaccggata ctgtcaaccg gggtttgtgc cccggaaata aaactgttca ttataacgca
     7021 aacaaaaagc agccatttaa ttttcttcat aacagtggtt ttagtgtagt cctggtcaac
     7081 caggcataga aattacgaaa gaaaatacaa tttcagcctt aaaataaatg tagttggatt
     7141 ttttacagcc cccaaagggg tcctttctat ccctgagcgt tttaaatgac cagggcaaat
     7201 tctgataaca cttcttttgt gaagacactg acaagggagg aggaatattt attgagccag
     7261 agatttccgg tgcatcaata attaagccag aagaattacc agttagtggt ataggaggtt
     7321 tgaaaaaagt gttttagttt tccgatagtt cattatttta aaacctttaa acctttttac
     7381 tatgctaaaa aattcattca tggggggggg tattaatact actctctttc cttgttacca
     7441 cggctaatgc caaaagcctt cc
//
LOCUS       DAORTI010000150         7457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11176_length_7457_cov_8.035531, whole genome shotgun sequence.
ACCESSION   DAORTI010000150 DAORTI010000000
VERSION     DAORTI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11176_length_7457_cov_8.035531"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLO70_14365"
     CDS             complement(<1..737)
                     /locus_tag="PLO70_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ75703.1"
                     /translation="MSTPKKTFFDFALLRRVFRYAAPYKKKFYLSILLAILLAIITPV
                     RPMLIQLTVNEYITNEIAAMLINITLIQIGLILIETAMRFFFSFTTAVLGQSVVKDMR
                     VATYEKIINLNLSQFDKTPIGTLTTRTINDIESINDIFSDGLVPILADLLSIVCVLVF
                     MFWTDWQLTFIALIPFPIMLIGTYYFKESINRSFHKVRNAVAALNAFVQEHLTGMAVV
                     QAFAAEDREFEKFKKINREHRNANIKA"
     gene            complement(813..3071)
                     /locus_tag="PLO70_14370"
     CDS             complement(813..3071)
                     /locus_tag="PLO70_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75704.1"
                     /translation="MIAITRYLIFLPLFLLALSVAKSQSFSSFDTLAIPAKYKPEFRR
                     ERNHELIDAEQKAILGSDGNRDNLFAPSDKEEINLMLSQSLVKKVDWLQYSIETDAGF
                     DHRLKVNYLFGLENLLKYFRQNWKQQSDRKINPLNLSAIISAYESCMNADRLGRSIEN
                     IVYPLSYDAGTNLINANIFEKNPGYQPVKDRLVLKLCQLHPGRIFSILQQNPDVPFAD
                     SLVRAIAKKYPRQLYDYAAAGNKLGVIIRNISDDMFIKTIATMARSKSGQQYFPFLDN
                     IVNGKLTLKEIDAAKDDSVLYYQLLVKTQMDYVARAINKDTAFEFKSLTRRLEDKAKA
                     DFINIINGLHNERNPEIRFRCIQALNAQELYYLAVSSDGSIYTSSFVKGVYPLMMQKI
                     NNRGDSLLMLLNFDKYRKFIKMSAGFNTLDHFLASFPKSDQPGEEDPANTLMRAFVKN
                     LEKSDGLEDGVDVADSYASIAETLKPVAERMLLNIQDNYRRTVASNNTKGMAIYNILN
                     KLFLSADTTQKIDLTKELGIPPVYEVPFSALGYDSGHVAVQLFIYGDKDGIGVFPGLI
                     SMFNNPNWKIDGSNKQWVTISSAKGRPVSLFMNRPLPEETGEDAKAQEALCQYLEKNG
                     IIPSVTINRGHSYNAPYTIEQMSAASKIVFMGSCGGYRMIHDILAKAPDAHIIGTKQI
                     ADAPVNNPFLKLIMEKLRAGSNIEWIPFWKELDKMVTDKIFEDYVPPHKNLGALFIKA
                     YTKAMGREEENQ"
     gene            3304..3981
                     /locus_tag="PLO70_14375"
     CDS             3304..3981
                     /locus_tag="PLO70_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75705.1"
                     /translation="METKARKVLIADDEPDILEILKYNLSNEGYEVITAKDGDEALEK
                     ARRSMPDLIVLDVMMPKKTGVEVCQLLRAQAAFKETLIIFLTAVNDEGTQIKGLETGA
                     DDYLSKPVSPKVFISRINALFRRLNKTEARILTIDNITIDPERFIVKVGENEIVLAKK
                     EFELLYLLAVKPGRVFLRNEILNQVWGNDVIVGDRTIDVHIRKIRQKLGIDCITTVKG
                     VGYKFEL"
     gene            4075..5124
                     /locus_tag="PLO70_14380"
     CDS             4075..5124
                     /locus_tag="PLO70_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75706.1"
                     /translation="MFSIKNISPRQIAVFTALVLAIPESILVLIVEKSWVWGVGSFLV
                     IFIGSYFLISFMLERFIYRKIKLIYKFIYKTKATRKEETYYKYVLPQKSIDEVREDVE
                     AWATKNEEELDLLRRNEQFRKEFLQNLSHEFKTPVFAIQGYVDTLLSGALANPEVNKK
                     FLEKTAKNVDRLTHLLNDLDEISKLERGELVLYKQNFVIQELVKDVFESLSGKAEAMN
                     IHSSIKKGCESPLTVFADKEKIRQVLTNLVENSIKYGKQNGSITASMYKTDGKHILIE
                     LSDDGIGISEKHLPRIFERFYRTEEGRSIDVSGSGLGLAICKHIVEAHGQTIHVRSRE
                     DIGTTIGFTLDARRD"
     gene            5208..7082
                     /locus_tag="PLO70_14385"
     CDS             5208..7082
                     /locus_tag="PLO70_14385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQZ75707.1"
                     /translation="MKLFLSISLILLIQQSITAQLPAIPSSYSNIGYDEKGRLFFSKE
                     GQRYYADTTGPRYTLQKLFGNPKGTDSGLSLDFGTFKGTVTYGMIPYGKVPHPLPIFR
                     ITAPLKDGKVNINIKNDFRNPYDFVKWSDNKGFSIGYRLMDEKGLVVFDGEVTAEGIG
                     PFTVVPAIYEGPFISNVTETSAVIWFNTNQPVQTSVLVDNRELKNEAAATHHEFKITG
                     LKPGTKYAYVVKAGSQSQAYHLTTSPKKGSRKPFVFAYTSDSRSATGGGERNIYGANT
                     YIMKKIAALAYQQGAAFMQFTGDMINGYLSNKEEQLVQYTNWKKAIEPFWHYIPFYAG
                     QGNHEALGYIFRDTAGAQRAFIDKFPYTIHSAEQAMREAFVNPENGPVSEDGSIYDPN
                     PEQVDFPSYSETVFYYTYDNTAMIVLNSDYWFAPSISRDTSTGGGLHGYIMDNQLEWL
                     RKTILQLEKDPAIDHIFVTQHTPVFPNGGHKGDDMWYNGNNDKRTYVAGKPVEKGIIE
                     RRDEYLDILINKSKKVVAVLTGDEHNYNRLKLTKNVTIYPDNYPHKKLNVSRHIYQIN
                     NGAAGAPYYAQEQLPWSEHTKAFSVENAVCLFYVSGKSIKMRVLNPDTLNQIDEITLR
                     "
     gene            complement(7098..>7457)
                     /locus_tag="PLO70_14390"
     CDS             complement(7098..>7457)
                     /locus_tag="PLO70_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75708.1"
                     /translation="SCGGSVVSKKLAVADSLVITFNIPNSDSVIKTVSTTEGKAIQKF
                     ISLVDGKKGELYKCGYDGNMVFFSKGEIVLPVIFKYTEEGCRHFLFDMDNKVMSTEMT
                     IEGAELLKSLVEGKEWY"
BASE COUNT         2260 a         1519 c         1518 g         2160 t
ORIGIN      
        1 atggctttga tattcgcatt acgatgctcc ctgttaatct tcttaaactt ttcaaattcc
       61 ctgtcttcgg cagcaaaagc ctgcactacc gccataccgg taagatgttc ctgcacaaat
      121 gcattcaatg cagctactgc attcctaact ttatgaaaag aacggttgat actttctttg
      181 aaataataag tgccgataag catgattggg aatggtatca atgcgatgaa ggtcagttgc
      241 cagtcagtcc agaacataaa caccagtaca cagactattg aaagcagatc agccaggatg
      301 ggaaccagtc catctgaaaa aatatcattg atggattcga tatcattaat tgtccttgta
      361 gtgagtgtgc caattggtgt tttgtcaaac tgtgaaagat tgagattaat tattttttca
      421 tacgtagcaa ccctcatatc ctttactacg gactgaccca atacagccgt tgtaaaggag
      481 aaaaaaaacc gcatggcagt ttcaattaat atcaacccga tttgaataag ggtaatatta
      541 atcagcatgg cggctatctc attggtaata tattcattga ccgtaagctg aattagcatt
      601 ggtcttaccg gcgtaataat agccagtagt atagccagta gtatagaaag ataaaatttc
      661 ttcttgtatg gtgcagcata gcggaacacc ctccgcaata aggcaaaatc gaaaaaagtt
      721 tttttaggtg ttgacaaagt gaatagttga agaagtctga ctccgcagac aagcagcaaa
      781 tttagacaga agtgaacaga aaggattacc gtttattgat tttcctcttc acgccccatg
      841 gcttttgtgt acgctttgat aaataaagca ccaagatttt tatgcggagg tacataatcc
      901 tcaaatatct tatcagtaac catcttatcc aactctttcc agaagggtat ccattctata
      961 ttacttccgg ctctgagttt ctccattatt aattttaaaa atggattgtt tactggtgca
     1021 tccgctatct gtttggttcc gatgatatgc gcatcgggtg ctttcgccag gatatcatgt
     1081 atcatgcgat aaccaccaca agaacccata aagacaatct ttgaggcagc agacatctgt
     1141 tcgatcgtat aaggagcatt ataactatga cccctgttga ttgtaacgga tggaataatt
     1201 ccgtttttct ccaggtactg gcacaaggct tcctgtgctt ttgcatcctc ccctgtttct
     1261 tcgggcaatg gcctgttcat aaaaagagag accggccggc cctttgctga ggaaatagta
     1321 acccactgct tgttactacc atcaattttc caattggggt tattgaacat actgatcagc
     1381 ccggggaaaa cacctatccc gtctttatcg ccgtaaataa atagctgaac agccacatgc
     1441 ccgctgtcat atcctaatgc actaaatggc acttcatata ccggaggtat ccctaattct
     1501 ttagtcaggt caattttctg agtggtatca gcagacagaa aaagtttatt taaaatatta
     1561 taaatcgcca tccccttcgt attgttactg gcaacagttc gccggtaatt atcctgaata
     1621 tttaacagca ttcgttctgc aacgggcttc aatgtttcgg caatacttgc ataggagtct
     1681 gctacatcaa ccccgtcttc caggccatca gatttttcca ggttcttcac aaatgccctc
     1741 atcaaagtat tggcggggtc ttcttcaccc ggctgatcac tctttggaaa agaagcaaga
     1801 aaatgatcca gtgtattaaa ccctgccgac attttgataa attttctgta cttatcaaaa
     1861 ttcaataaca ttaacaggga gtcgccccgg ttattgatct tttgcatcat caaaggatat
     1921 acccctttta caaagctgga tgtataaata gaaccatcag acgatacagc caggtaatac
     1981 aactcctgtg cgttcagtgc ctgaatgcac ctgaaacgaa tttcagggtt tctttcatta
     2041 tgcaagccgt taattatatt gataaaatca gctttcgctt tatcttccag tctccgcgtc
     2101 aatgacttaa actcaaatgc ggtgtcctta ttgatggccc tggccacata atccatctgt
     2161 gtctttacca gcagctgata atagagtact gaatcatctt tggctgcatc gatttctttc
     2221 agtgttaatt ttccgtttac aatattgtca aggaaaggaa aatattgctg gccactttta
     2281 cttcgggcca tagtggctat tgttttgatg aacatatcat cagaaatatt ccggatgatt
     2341 acacctagct tattaccggc agctgcatag tcatataatt gtcttgggta tttttttgca
     2401 atggccctga ccaggctgtc cgcaaagggt acatctggat tttgttgcag aatcgaaaag
     2461 attctgccag gatgaagctg gcaaagttta agcaccaacc tgtcttttac cggctggtat
     2521 cctggatttt tttcaaaaat atttgcattg atgagattcg tccctgcatc atacgacaaa
     2581 ggataaacaa tattctcgat ggaccgaccc agcctgtctg cattcatgca ggactcataa
     2641 gcgctgataa tagcggaaag gttcaatgga ttaatttttc tatccgattg ctgtttccag
     2701 ttttggcgaa aatattttaa caggttttca agtccaaaga ggtaattgac ttttaaccgg
     2761 tgatcaaatc cggcatccgt ttcaatacta tattgtaacc agtccacttt cttaacgagg
     2821 gattgggata acatcaggtt aatctcttcc ttgtcgctgg gtgcaaataa attatcccga
     2881 tttccatccg aaccgagaat agccttttgt tctgcatcta tcaattcatg gtttctttcc
     2941 cgcctgaatt caggtttata tttggccggt atggcaaggg tatcgaaact gctaaagctt
     3001 tggctttttg caacagaaag ggccaataag aaaagaggga gaaaaataag gtatctggtt
     3061 attgctatca taaggatgag gcgtaaagtt actaagccct gttgaaagtt tcattttagc
     3121 ttttgctgtt gatacagacg gctttacaat gcataaagct gtctgagacc tgttaaatat
     3181 ttttattgcc gctttggtag tgacctcatt tatattaagt tatcattaag tctgtgtaaa
     3241 cgactgcctg taaatttccc tgctagtcat tttcccgtta ggtttgcgca aattatttcc
     3301 agtatggaaa ccaaagcccg gaaagtactg attgctgatg acgaaccgga catccttgag
     3361 atcctgaaat acaacctttc caacgaaggt tatgaagtca ttactgctaa agacggtgat
     3421 gaagcgctgg aaaaagcccg tcgttctatg cccgatctca tagtgctgga cgtaatgatg
     3481 cccaaaaaaa caggtgtgga agtttgccag ctgctaaggg cacaagctgc ttttaaagaa
     3541 acgctgatca ttttcctgac agcggtaaat gacgaaggca cacagataaa aggattggaa
     3601 accggtgcag atgactatct gagtaaaccg gtaagtccaa aagtttttat aagccggatt
     3661 aatgcattgt tccgcaggtt gaacaaaacg gaagcacgga ttctgactat tgataatata
     3721 accattgacc cggagcgatt tattgtgaaa gtgggagaaa atgagatcgt gctggctaaa
     3781 aaagaatttg aattgttgta tctgctggcg gtaaagcccg gccgtgtttt tcttcgcaat
     3841 gaaatactta accaggtgtg gggcaatgat gtgattgttg gcgaccgcac aatagatgtt
     3901 catatccgca agatcaggca gaagttaggt atcgattgca taacaacggt aaaaggagtt
     3961 gggtataagt tcgaactcta acccatctac cttatgcaat aagctttccg ctttttgctt
     4021 tttgcctttt gcatttccta ctgcactcaa ccttatccct tgtaccttcg taccatgttt
     4081 tctattaaaa atatttctcc aagacagata gctgtcttta ctgccctggt actggcaata
     4141 ccggagagta tccttgtatt aatagtagaa aaaagttggg tatggggggt tggctccttt
     4201 ttagtaatct ttattggcag ttattttctt atctctttta tgctggaaag gtttatctac
     4261 aggaaaataa aactcattta taaattcatt tacaaaacca aggccaccag gaaagaagaa
     4321 acctattaca aatatgtatt acctcaaaag agtatagatg aggtccgtga agatgtcgaa
     4381 gcatgggcta caaaaaatga agaagaactg gacctgctac gccgtaatga acagttccgt
     4441 aaagaatttt tacaaaatct ttcgcatgaa tttaaaaccc ctgtttttgc catacaaggc
     4501 tatgtggaca ccctgttgtc cggggcattg gctaatcctg aggtaaataa aaaatttctc
     4561 gagaaaacgg caaagaatgt ggaccgcctg actcacctgc tgaacgacct tgatgaaatt
     4621 tcaaaactgg aaagaggtga actggtactc tacaaacaaa attttgtgat ccaggaactg
     4681 gttaaagatg tcttcgaaag cctctcaggc aaagcagaag caatgaatat tcatagcagc
     4741 ataaaaaaag gatgcgaatc gccgcttacg gtctttgcag ataaagaaaa gattcggcag
     4801 gtacttacca acctggtgga gaactccatt aagtatggca aacaaaacgg gagcattacc
     4861 gccagtatgt ataaaacaga tgggaaacat atattaatag agttaagtga tgatggaatt
     4921 ggtatcagtg aaaaacacct gccccgtatt ttcgaacgct tttaccgtac cgaagaaggc
     4981 cggagcatag atgtatccgg aagtggcctg ggtttggcga tctgtaaaca tatagtggag
     5041 gcgcacggac aaaccattca tgtacgcagt cgggaagaca ttggcaccac aattggattt
     5101 acattagatg caagaaggga ttaatatcaa cggctccctg caacttatta attttacctt
     5161 ttccggataa tagctggtta tttacaaata ataattaacg atacaatatg aaactttttt
     5221 tgtccatcag tcttattctt ttaattcagc aatcaataac agcacaatta ccggctattc
     5281 cttcgtctta ttctaatatc ggatacgatg aaaaaggaag attatttttt tctaaagaag
     5341 gtcagcgata ttatgcagat accacaggtc cccgctacac acttcagaaa ttatttggta
     5401 accctaaagg aactgacagt ggtttgtcat tggattttgg cactttcaaa gggacagtga
     5461 cctatggcat gataccttac gggaaagtgc ctcatccctt accgattttt cgcattaccg
     5521 ctccgttgaa agacggaaaa gtgaatatca atatcaaaaa tgatttccgt aatccttacg
     5581 attttgtgaa gtggtcagat aataaaggtt tttctattgg atatcgcctg atggatgaaa
     5641 aaggattggt ggtgtttgac ggtgaggtta cagcagaagg tattggccct tttactgtag
     5701 tacctgcaat atatgaaggt ccgtttataa gtaatgtgac cgaaaccagt gctgttatct
     5761 ggtttaatac aaaccaaccc gtgcaaacaa gtgtgctcgt tgataataga gaattaaaaa
     5821 atgaagcagc tgccacacat catgagttca aaataaccgg gctgaagccg ggtacaaaat
     5881 atgcgtacgt tgtgaaagca ggttctcaat cacaagctta tcacctcaca acttctccca
     5941 aaaaaggtag ccgcaaacct tttgtttttg cctataccag cgacagccgc agtgctaccg
     6001 gtggcggtga aagaaatata tatggtgcaa atacatatat tatgaaaaag attgcagcgc
     6061 ttgcttatca gcagggggca gcttttatgc agttcaccgg cgatatgatc aacggctatt
     6121 tatctaataa ggaagaacag cttgtccagt acactaactg gaagaaagcc attgaaccat
     6181 tctggcatta tatacctttt tatgccggac agggaaacca tgaagcgttg gggtatattt
     6241 tcagggatac tgccggtgcc cagcgggcat ttattgataa gtttccttac actattcatt
     6301 cggcagaaca ggccatgcgg gaagcatttg taaacccgga gaacggaccg gtaagcgagg
     6361 atggcagtat ctatgacccc aatccggaac aagttgattt cccttcttac agtgaaacgg
     6421 tattctacta tacttatgat aatacagcaa tgattgtact gaacagtgat tactggtttg
     6481 ccccatctat tagcagggat acatcaaccg gtggcgggct tcatggctat attatggaca
     6541 accagttgga gtggcttcgt aaaaccatac tgcaactgga aaaagacccg gccatagacc
     6601 atatattcgt tacacagcac accccggttt tcccgaatgg cggacataaa ggtgacgata
     6661 tgtggtataa tggaaataat gataaaagaa cctatgtagc aggtaaaccg gtggaaaagg
     6721 ggatcattga aagaagagat gaatacctcg atatactgat caataaaagt aaaaaagtgg
     6781 tggcggtcct tactggagat gagcataatt acaaccggct gaagctgaca aaaaatgtca
     6841 ccatttaccc ggataattat ccgcataaaa aactgaatgt ctcccggcat atttaccaga
     6901 tcaataatgg tgctgccggt gctccttact atgcacagga acaattgccc tggagtgaac
     6961 acacgaaagc attcagtgtt gaaaatgcgg tatgcctttt ttatgtaagc gggaaaagta
     7021 taaagatgag agttctcaat ccggatacct taaaccagat tgatgaaata acacttcgct
     7081 gacaggcaac taaaaactta ataccattct ttcccttcaa caaggctttt cagtaattcg
     7141 gccccttcaa tcgtcatttc tgtactcatc actttgttat ccatatcaaa caaaaagtga
     7201 cgacaaccct cttccgtgta tttaaagatc accggcaata caatttcacc tttgctgaaa
     7261 aagaccatat taccatcata cccgcatttg tacaactccc ctttcttacc atctaccagg
     7321 ctgataaact tttgtattgc ttttccctcc gttgtactaa ctgttttaat tactgaatca
     7381 ctgttgggaa tattaaaagt gataacaagg ctatcagcaa cagctaattt tttgcttaca
     7441 acagaaccac cgcagga
//
LOCUS       DAORTI010000151         7438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11211_length_7438_cov_6.240553, whole genome shotgun sequence.
ACCESSION   DAORTI010000151 DAORTI010000000
VERSION     DAORTI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_7438_cov_6.240553"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..256
                     /locus_tag="PLO70_14395"
     tRNA            184..256
                     /locus_tag="PLO70_14395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:217..219,aa:Lys,seq:ttt)
     gene            complement(254..1102)
                     /locus_tag="PLO70_14400"
     CDS             complement(254..1102)
                     /locus_tag="PLO70_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75709.1"
                     /translation="MKYIIFIPILFFLSAIAAHAQEGDNSVSVSAARYIPSRTSIAFQ
                     VGNRKIPITVLQYGEANGIVCINVHDNETASVEGAKAVLEITGGTLIKIENYKQRVIR
                     FKLNGISYGFDPNRIYSRVGIGQTLRDNRRVSKPAMDEIEKFAQRVLELIPDSASCIV
                     ALHNNTEEAFSIKSYMTGGNRQKDARAVYANDQQDVDDIILTTDSLLYQKMADAGYNS
                     IWQDNERAKKDGSLSIHFGERARRYINIETQHGKVEQYKEMFEKLMAILAEEKEKSPG
                     EPKDSL"
     gene            complement(1191..2129)
                     /gene="plsX"
                     /locus_tag="PLO70_14405"
     CDS             complement(1191..2129)
                     /gene="plsX"
                     /locus_tag="PLO70_14405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQZ75710.1"
                     /translation="MTIGIDMMGGDFAPLEAVKGINLFLSNHNIPPSLLLIGDKEQVE
                     ALLAEHKVPVANIKTVHAEQVIDMHEHPTRALKEKQKSSIAIGFHLLASGKIDAFISA
                     GNTGAMLVGALFSIKAIEGVIRPTISTIIPKIGGGSGLLLDVGLNADCKPEQLNQFAI
                     MGSVYAQLILGIDNPKVGLINVGEEEGKGNLLAQATYPLLKENKHINFIGNIEGRDVL
                     MEKADVMVCEGFTGNIILKLAESLYEIAQQQAVNNQYFDRFNFENYGGTPVLGVAKPV
                     IIGHGISGAKAFMNMIYLAQQMIEKDVMKKMKEELS"
     gene            complement(2223..2420)
                     /gene="rpmF"
                     /locus_tag="PLO70_14410"
     CDS             complement(2223..2420)
                     /gene="rpmF"
                     /locus_tag="PLO70_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQZ75711.1"
                     /translation="MPNPKRRHSQQRSAKRRTHYKATAATLTTDSATGETHVRHRAHV
                     SEGKLYYKGKVVAEKAPLRAQ"
     gene            complement(2494..3039)
                     /locus_tag="PLO70_14415"
     CDS             complement(2494..3039)
                     /locus_tag="PLO70_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQZ75712.1"
                     /translation="MSRRREFEIAYVGLKPGVHEYNYEINDKFFEAFQQQDFRNCKAN
                     VKLSLDRKSSFMLLKFEIGGTLEVTCDRCNNNLPLNLWDEFNITVKMVEEPELMNNQE
                     EDPDVYYISRGESHIDVAEWIYEFISLSLPMQKTCEYEKMDGPYCNKAALEMLKKMEV
                     KETGKKDNPIWKGLEKFKDLE"
     gene            3169..4281
                     /locus_tag="PLO70_14420"
     CDS             3169..4281
                     /locus_tag="PLO70_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HQZ75713.1"
                     /translation="MQQFLTRLFGRLMKSWSWVLLIILTILIKWVSWYPDWVEINYSQ
                     GVYPVISSVQRFLFGWIPFSVGDLFYGFLVLVVVFRTYKFFKFLFLRKLTRQFFVIAM
                     QQGIFFLLFVYVFFNLLWGLNYNRKGIADQLELQVKGYTLADLDTLTTILQDRVNGYA
                     PFVNESQRDSFDRKRTLFSNTAEAYKEAAKQFSFLKYSPKSIKPSLFSYLGNYLGFQG
                     YYNPFSGEGQVNTTIPRFLEPFVTAHEVAHQIGYAKENEANFAGFLACRSYPSNVFRY
                     SMYLDMYNYALGEVYRRDTSLARAFQNKAHPQIIKDQKEFRDFYQRYNNPVEDLIMWG
                     YGHFLKANNQPAGKRSYNEVVSWLVAYYKKFGTMAL"
     gene            4313..4786
                     /locus_tag="PLO70_14425"
     CDS             4313..4786
                     /locus_tag="PLO70_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQZ75714.1"
                     /translation="MNNTLKLIIAIAIPLIVGGTSGFFTATGVESWYQTIARPTWNPP
                     GWIFGPVWTTLYVMMGISLFLVWKEDTSVELKKIAIALFTVQLVLNFFWSFIFFNQHQ
                     IGWALVEIIAMWVFILLTIFAFAQVNKAAAWLLVPYISWVSFATILNFTIWQLNK"
     gene            complement(4846..5382)
                     /gene="dcd"
                     /locus_tag="PLO70_14430"
     CDS             complement(4846..5382)
                     /gene="dcd"
                     /locus_tag="PLO70_14430"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQZ75715.1"
                     /translation="MILSDTRILEEMEKGTIKVTPYNRECLGSNSYDVHLGKTLATYK
                     EHMIDAKKHNQIDYFEIPDEGIVLYPHIFYLGVTEEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVKVYKGMPIGQLIYFPVDGEIEVKYNQKKNSK
                     YSNQPDKPVESMMWKNNF"
     gene            5436..6053
                     /locus_tag="PLO70_14435"
     CDS             5436..6053
                     /locus_tag="PLO70_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQZ75716.1"
                     /translation="MPVFFQHQINENTRLGVWKIEESEAFFKGNVPLHRHVTHPHKRL
                     QHLAGRFLLQFLFPDFPYELVQIADTNKPFLPEEQYHFSISHCGDYAAAIVSRDKRVG
                     IDVELTTNKIVKIKDKYLSKEEQHLFTTELLTTDYHLLTLLWSAKESVFKWYGQGNVD
                     FKKHIRLLNHQEGKETLDCFFSKNESELTIHYRWYDDLVLSWIIN"
     gene            6105..6746
                     /locus_tag="PLO70_14440"
     CDS             6105..6746
                     /locus_tag="PLO70_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75717.1"
                     /translation="MKIQITLFLFTSLVLASCGPKIYKSTEFDTALARHKTVAILPAD
                     VSTRLRPNEAKKVTAEQLAEMNEKTGFAIQDKMYSWFLRRSDKYQYTVTFQDVTRTNA
                     LLKQAGIGYNDLAAKDRTELAKLLGVDAVLQDRTSMDKPMSDGAAVAVGVIFGMWGST
                     NSVQTTINIHDGKTGNLLWKYDYQAQGSVGSSAENLVNALMRNASKKFPYSVK"
     gene            complement(6831..>7438)
                     /locus_tag="PLO70_14445"
     CDS             complement(6831..>7438)
                     /locus_tag="PLO70_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HQZ75718.1"
                     /translation="DLIAEVGKGVRGADCIQTIRNSFGQECGKIIFESKRTKDFSNDW
                     IEKLKADMRSQGADVAVIITQALPKEMTNFGEKDGVWICSFAEAKAVVHMLRDAVIKI
                     ANASKSQENRGDKMHLLYSYLTSREFAEQWQAIREGFMSMKLSIQKERDAMEKLWKQR
                     EKQLEKVLLNAAHVRGSIEGISGTDVDLNLLGDGNDDLQLD"
BASE COUNT         2056 a         1547 c         1462 g         2373 t
ORIGIN      
        1 ctgacatgat gattgattat gccacaaacc tacttcaaaa atgtgcaaat tgtaaatggc
       61 attcagttgt tttaaaacag cctgataagt tggtgggaga aactatgttt ataattgatt
      121 gaaaaagttt tggtatgaaa gccatgagac cgtatttttg cactcccaaa aagggaaagg
      181 gttgatctcg tagctcagtt ggtagagcac aacactttta atgttggggc cctgggttcg
      241 agtcccagcg ggatcacaga gagtccttcg gttcgccggg ggacttttct ttttcttcag
      301 ccagtatcgc catcaatttt tcaaacatct ctttatactg ttctactttg ccatgctgtg
      361 tctcaatatt gatatagcgg cgggctcttt ctccaaagtg aatactcaat gaaccgtctt
      421 tttttgctct ttcattatcc tgccagatac tgttgtagcc ggcatcggcc attttctgat
      481 acaaaaggct gtcggtagtt aaaataatat catccacatc ctgctgatca tttgcataaa
      541 cggccctcgc atctttttgc cggttaccgc cggtcatata gcttttgata gagaaggctt
      601 cttctgtatt gttatgtaat gcaactatgc aggatgcaga atcaggaatt aactccagta
      661 cccgttgtgc gaatttttca atttcatcca ttgccggctt acttaccctt cggttatctc
      721 taagcgtttg gccaatacca accctggaat atatcctgtt tggatcaaag ccataggaga
      781 tgccatttaa tttaaaacgg atcacccgtt gcttgtaatt ttcaattttt attaaagtgc
      841 cgcctgtaat ttccagtaca gccttagcac cctccacaga agcagtttcg ttatcatgta
      901 cattgataca aactatacca ttggcctcgc catattgtaa tactgtaatg ggtattttcc
      961 tgttgccgac ctggaaagcg atagatgtcc gggaaggtat ataccgggca gcagaaacag
     1021 atactgaatt atcaccttcc tgtgcatgtg cagctattgc gctgagaaaa aaaagtatcg
     1081 ggatgaaaat gatatacttc aatgttcgaa ttttcctaaa gataatattg cttttgaatt
     1141 attttatacc gggaaaaggg tatttaggcg gaaaggtgaa aactgccgga tcagctgagt
     1201 tcttctttca tcttcttcat cacatctttc tctatcatct gctgtgcaag ataaatcatg
     1261 ttcataaagg ctttggcgcc tgatatgcca tggcctatga taaccggttt cgcaacacct
     1321 aataccggag tgccaccata gttttcaaaa ttgaagcgat cgaaatattg attgttgacg
     1381 gcttgctgtt gtgcaatttc gtataatgat tctgccagtt tcaggatgat attacctgtg
     1441 aatccctcgc aaaccattac atctgctttt tccatcagta cgtcacgacc ttcaatattg
     1501 cctataaagt tgatgtgttt gttttctttc aacaggggat aggtagcctg ggccagtaag
     1561 tttccttttc cttcttcttc ccccacattt atcaatccaa ctttaggatt atctattccc
     1621 aggataagct gggcataaac tgagcccatt atggcgaact ggttcaattg ttccggcttg
     1681 caatcagcat ttaatccaac atccagcagc aagccgctcc caccgccaat tttagggatg
     1741 atggtagaaa tggtaggtcg tataacacct tcaattgctt ttatactgaa caaagctcct
     1801 acaagcatgg caccggtatt accggcactt ataaaagcat ctattttgcc ggaagccagt
     1861 aaatggaaac caatagcaat ggaagatttt tgtttttctt ttaaagccct ggtgggatgc
     1921 tcatgcatgt ctattacctg ttctgcatga actgttttga tatttgcaac cggaacctta
     1981 tgttctgcca gcagggcttc tacctgttct ttatcaccaa tcaggagaag tgagggtgga
     2041 atattatgat tagataaaaa aagattgatc cccttaactg cttccagcgg ggcaaagtca
     2101 ccacccatca tgtcaatgcc gatagtcata tcaagtaaat tacaaaacga caaattccaa
     2161 attccatcat aacggttacg ttaaatggat ttggaattaa atattgataa accggacaat
     2221 gtttattgtg ccctgagagg tgctttttca gcaactactt ttcctttgta gtacaattta
     2281 ccctcactta catgtgcacg gtgacgaaca tgtgtttcgc cggttgcgct gtctgttgtt
     2341 aatgttgcag cagtagcttt ataatgtgtt ctgcgcttgg cgcttctttg ctgactgtgt
     2401 ctgcgtttcg gatttggcat aactcaacta ttaaaattca aaaatatatt tatacttatc
     2461 agtttctttg atcataccgg gacaggtaac agattactct aaatctttaa acttttccag
     2521 tcctttccag atcggattat cttttttccc ggtttccttt acttccattt tcttcagcat
     2581 ttccaacgcc gctttgttac aataggggcc atccatcttt tcatactcac aggtcttttg
     2641 catgggaaga ctcaggctga tgaattcata tatccattct gccacatcaa tatgactttc
     2701 tccacggctg atataataaa catcaggatc ttcttcctgg ttattcatca gttccggctc
     2761 ctcaaccatt tttacggtga tgttgaattc atcccataag ttaagtggca ggttattgtt
     2821 gcaacggtcg caggtgacct ctaatgtgcc gccaatctca aatttcagca gcataaagct
     2881 gcttttcctg tccagggaca attttacatt ggccttgcaa ttgcggaaat cctgctgttg
     2941 aaatgcctca aagaacttat cattgatctc atagttgtac tcatgaaccc cgggttttaa
     3001 tcccacataa gctatctcaa actcccggcg gcgactcatt tgtcaacttt ttaaagagtg
     3061 tgcaaaggta agggaataat tggaaagtta atggctgtat ttcgattatt ttcgcactat
     3121 tataaaggcc taacagcctg attttgaagg taattaaatt ttcaccactt gcaacagttt
     3181 ttgacacgat tatttggccg tcttatgaaa agctggagtt gggtactcct gattatattg
     3241 accatcctga taaagtgggt ctcctggtat ccggattggg tcgaaataaa ttacagccag
     3301 ggagtttatc cggttatatc cagcgtacaa agattcctgt ttggatggat tccctttagt
     3361 gtgggtgatc ttttttacgg attcctggtc ctggtggttg tattccgcac ctataaattc
     3421 ttcaaattcc tgtttcttcg aaaactgaca agacagtttt tcgtaatagc catgcaacag
     3481 ggtattttct ttttactttt tgtatatgta ttttttaatc ttctttgggg gctaaattat
     3541 aaccggaaag ggatcgctga tcaattagag ttgcaggtga aggggtatac tttagccgat
     3601 ctcgacacct taacaactat tcttcaagat cgggtaaatg gatatgcccc ttttgtgaac
     3661 gaatcgcagc gggattcatt cgacaggaaa agaacgctat tcagtaatac agcagaggcc
     3721 tataaagaag cagcaaaaca attttcattt ctaaagtatt caccaaagtc aattaagcct
     3781 tctttgttca gctacctggg gaattatctg gggttccagg gatactacaa tccattttcg
     3841 ggtgaagggc aggttaatac tactattccc cgtttcttag agccgtttgt aacggcacat
     3901 gaagttgcgc accagattgg atatgcaaaa gagaacgaag ccaattttgc cgggttcctt
     3961 gcctgtcgta gttatccctc caatgtattc aggtattcta tgtacctgga tatgtataac
     4021 tatgcattgg gtgaagtgta tcgcagggat acttctcttg ccagggcttt ccagaataaa
     4081 gcacaccccc aaattatcaa agaccagaaa gaattcaggg atttttacca gcgctacaat
     4141 aacccggttg aagatctgat catgtggggc tatggtcatt ttctgaaagc aaataaccag
     4201 ccagccggga aaagaagcta caatgaagtg gtatcctggt tggtagctta ttataaaaaa
     4261 ttcggcacaa tggcattata aacaaatatt aacagcggtt gtatatttac atatgaataa
     4321 tacccttaaa ctgataattg ccattgcaat tccattaatt gttggcggta ccagtggttt
     4381 ttttacagca acaggtgtgg aaagctggta tcaaactatt gccaggccca catggaatcc
     4441 ccccggatgg atatttggcc cggtctggac gactttatat gttatgatgg gtatttcttt
     4501 atttcttgtt tggaaagaag ataccagtgt agaattaaaa aaaattgcta ttgcgctgtt
     4561 tacagttcag cttgtcctga attttttctg gtcctttatt ttttttaacc agcatcaaat
     4621 aggctgggcc ctggttgaaa taatcgctat gtgggttttt attctgctga ccatttttgc
     4681 gtttgcacaa gtgaataagg cagcagcatg gttactggtc ccctatataa gctgggtgag
     4741 ttttgcaacg attttgaatt ttacaatctg gcaacttaat aaataaaaaa ctgctcctgg
     4801 tttaccgggc agttttttat actcttttaa tcaatttaac gcggattaaa agttattctt
     4861 ccacatcata ctctcaactg gcttatccgg ttgattgcta tattttgagt ttttcttctg
     4921 gttgtatttc acttcaattt ccccatccac cgggaaatag atcaactgac caataggcat
     4981 tcctttatat actttcaccg gttgttttac tgaaatttcc agtgtccagt tgccacagaa
     5041 accaacatca ccttttcctg ctgtagcatg tatatctatt ccgagacgac cagtggatga
     5101 tttaccttcc aggaatggta catgggcatg tgtttctgta tattcttctg taacgccgag
     5161 gtagaaaata tgtggataca aaacgatgcc ttcatcagga atttcaaagt aatcgatctg
     5221 gttgtgcttt ttcgcatcaa tcatgtgctc tttgtatgtt gctaatgttt ttcccaggtg
     5281 cacatcgtag gagttactcc cgaggcattc acggttatag ggagtgactt taatagtgcc
     5341 tttctccatt tcttccagta tccgggtatc agaaagtatc attttatatc ttcggtttta
     5401 tttaggatgg caaagtaaac aggaatgaag aactaatgcc ggtttttttt caacatcaaa
     5461 tcaacgaaaa tacccgcctg ggggtatgga agatcgaaga atcagaagcg ttctttaaag
     5521 ggaatgtgcc cctgcaccgt catgttactc atcctcataa acggttgcag caccttgccg
     5581 gacgattctt attacaattc ctgttcccgg actttcctta cgaactggtt cagattgctg
     5641 ataccaataa gccttttctt ccggaagaac agtatcattt ttctatttcg cattgcgggg
     5701 attacgctgc agcaattgta agcagggata aaagggtagg cattgatgta gaattgacga
     5761 caaataaaat tgtaaagatc aaagacaaat accttagcaa ggaggagcag catttgttta
     5821 ctacagaatt actgacaacc gactatcatt tactgactct tttatggtct gctaaagaat
     5881 ctgtttttaa atggtatgga caaggtaatg ttgactttaa gaagcatatc cgtttattga
     5941 atcaccagga aggaaaagag acgttagact gcttcttctc taaaaatgaa tctgaactaa
     6001 ctattcatta ccgctggtat gatgatttag tactttcgtg gattattaac taattattat
     6061 ttggctatta cagaccatct ttttttattt gtaaaaaatt atatatgaag attcaaatca
     6121 cccttttcct ttttaccagt ttagtactgg catcctgcgg cccgaagatc tacaaatcca
     6181 cagaatttga caccgcactt gcccgtcata agacggttgc catacttcct gcagatgtaa
     6241 gtacccgttt acgaccgaat gaagccaaaa aagtaacagc agaacaactg gcggaaatga
     6301 atgagaaaac cggttttgcc atccaggata aaatgtacag ctggttttta cgccggtcag
     6361 ataaatacca gtacacagta acattccagg atgttacaag gacaaatgcc cttttaaaac
     6421 aagcgggtat tggttataac gacctggcag ctaaagacag aactgaactt gcaaaacttc
     6481 tcggcgtaga tgcagtgctg caggacagga ccagtatgga caagcctatg tctgacggtg
     6541 ctgctgtagc agttggtgtt atttttggta tgtggggaag tactaacagt gtacagacca
     6601 ccataaatat acatgatggt aaaacgggta acctgctttg gaagtatgac taccaggccc
     6661 agggatccgt tggttcttct gcagaaaacc tcgtgaatgc gttaatgaga aatgcatcca
     6721 agaaattccc ctatagtgtg aagtaataaa gaatgattta tcagcaataa agccaccatg
     6781 atactggtgg ctttattatt taatatactt aaaagagatg tagaaacaga ttagtctaac
     6841 tgaagatcat cattgccatc tcccaataaa ttaaggtcta catcagttcc ggaaatgcct
     6901 tcaatagaac cacgtacatg agccgcattg agcaaaactt tctccaactg tttctccctt
     6961 tgtttccata atttttccat tgcatctctt tctttttgta tagagagttt catgctcatg
     7021 aatccctccc ggatggcctg ccattgttcg gcaaattcac gactggtcag gtaactataa
     7081 agcaggtgca ttttatcgcc cctgttctcc tggctttttg aagcgttagc tatcttaatt
     7141 actgcgtccc gcaacatatg aacaacagct ttcgcctctg caaatgaaca gatccacact
     7201 ccatcttttt caccaaagtt ggtcatttcc ttaggtaatg cctgggtaat aattacagcc
     7261 acatcagccc cctggctccg catatcagct tttaattttt caatccagtc attggaaaaa
     7321 tctttggttc gtttactttc aaatatgatt ttaccacatt cctgcccaaa actattacga
     7381 attgtttgaa tacaatctgc cccgcggacg cctttgccaa cttctgcaat caggtcaa
//
LOCUS       DAORTI010000152         7275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11615_length_7275_cov_5.672161, whole genome shotgun sequence.
ACCESSION   DAORTI010000152 DAORTI010000000
VERSION     DAORTI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11615_length_7275_cov_5.672161"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(626..1900)
                     /locus_tag="PLO70_14450"
     CDS             complement(626..1900)
                     /locus_tag="PLO70_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ75719.1"
                     /translation="MKKYILFPFTLVLIAILLTSCTSKSADNSNLGDGKNQASQKGNA
                     KKEHGHDEHPHEDSANKGHNDAEEMNAVHLSTQKFNSLNIKVDTIPIRALSGVVNVNG
                     RLELFPQHKAIVTAILGANVIDIKVIEGEKVKKSQVLAYLSHPNLTNLQIAYIKVYNQ
                     MQFLEKEYVRQKRLYDEGIGSGKTYQQTQADYQTIKGEAKGLETQLKQLNIEVRKVRD
                     GNIVQYVPVVSPIDGYIEKVLIQTGQFIDPQAPMFGVINNDYIHADLMVFEKDIYKVK
                     KGQKVSFTVQSVPDKNLSAIIFSVEKNFERKPMAVRVHAEIDQKEHFLIPGMYIRGKI
                     HTESESVKALPEEAIIIEEGKPYIFMAHTRQESGKMEWVFSPVEIRTGISSEGWVEIN
                     LLEPLPMNAKVAWNNAYYLIGEMKKGETSHEH"
     gene            complement(1934..6361)
                     /locus_tag="PLO70_14455"
     CDS             complement(1934..6361)
                     /locus_tag="PLO70_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQZ75720.1"
                     /translation="MINSIIYFSIKNKLIVGLLTLALIGAGIYSLFTVNLGTVPDITN
                     NQVQVITVSQNLATEDIERFVTFPVELAMANLPGVTEIRSISRFGLSVVTIVFEDDMG
                     TYLPRQLVQEKLNVVIQQIPENFGSPSMGPITTGLGEIYQYTLQPKPGYETAYSPTEL
                     RTIQDWIVRRQLALVEGVVGINAFGGKIKQYEIAINPENLNANNVSISEIFEALRRNN
                     NNTGGAYIEKNNMANFIRVEGLVRSLDDIGNIAIKDVNGIPVLIRDVVEKIHFGHQVR
                     YGAFTQDGREAVGGMILMLRGANSNAVIQNVQKRIAEVQKSLPEGLEIRPFLDRSALI
                     ERTTSTVRENLTEGALVVIFALVIILGSIRGGIVTASTILLSFLFAAILMKLFNVSAN
                     LISLGAIDFGIIVDGAVIIVERTIYEIQKKFKAGKFDIDQDVMDELAYQAGSSMMNTA
                     IFGQLIILIVFVPILFLTGIEGKMFHPMAYTFGFTMLGAIILCLTYVPAMSALFLKPL
                     KNKKNWSGKLERSLEKVGEKIINGFQLVYLPLLKSALRHQAIVMIAAIVLLAAAIFTF
                     TRLGGEFIPQLEEGDIAMQALIRPGSSLSEAIDVSIKIENILLENFPEIKIALARIGV
                     AEIPTDPMPMDIADMFIILEKDKGKWISAKSKDELIEKIKEKLDNELTGVNLVFSQPI
                     QLRFNELLTGVREDVAVKLYGDDLDVLAQKANEMAKIIETIPGVGDVNPERTAGLPQM
                     TVRYNREKIAQYGLNIDKLNEYVRSAFAGGVAGVIFEGEKRFDMVIRFDKTHQKSIDD
                     LRNLYIDLPNGNQVPIKELADINFVLGPMQISRDNVFRRTYVGVNIRGRDMESVVKDI
                     QQKLGAELNLPPGYYLIYGGEFENLQKAKNRLAIVVPITIFLIFVLLYFALKSFSQSI
                     MLFTAIPLAAIGGVFALWLRDIPFSVSAGVGFVVLFGVAVLDDLVLISRFNSLKEEGI
                     IDIEERILIAVKERIRPILLTDATDILGFLPMALSVSAGAEVQHPLATVVIGGLISGT
                     LLTLIVLPVIYAFVENRSNNKKNLISLKRPNTNVLFILIVCGGLLSILLGTASTVNAQ
                     TQPIDSLQTITLEQAKELAVQNFPKIQAARLEIENQEVQKKTAWDLGSTSIFTGAEKI
                     GYTPETTYMNVGIKQSNINIFGIVPKLELQKERVALAEKALNLSVIEVEREVSRAWAM
                     AYTAKNNYQVYKRLDSIFMDIERAARIRLEVKATSKLEYLATSNQGNQVQIQKEQAYR
                     DYLSALHRLNLWFVTDTVFTVPDIPANQLESPLNLISDSIDAHPVLNVSKQKVSVAEA
                     AIKERRSQFLPKLSLQYGKQEISGLPGLHQFQVGIEVPLFFAPELGRSQAAKIEHTIA
                     NQNLQQSQLELAAAYQNIREQYIKWLNSWQYYRDTALPMAREQQTGAVTAYQEGAIDY
                     VTFLQNMREAIRIEVDSWNAFGNYLDSRYQLEYFLKTSN"
     gene            complement(6659..6997)
                     /locus_tag="PLO70_14460"
     CDS             complement(6659..6997)
                     /locus_tag="PLO70_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75721.1"
                     /translation="MLVLSLSLLANTLAWAFHGEVFAHELDRHNLTHSAAQTMQEDHL
                     TNELSDEKNLDLSAHICLSAAYQPFFFAKFPLLVPIAGKEIPVKLILSQVPESFIDSP
                     FRPPRKIIHS"
BASE COUNT         2020 a         1571 c         1426 g         2258 t
ORIGIN      
        1 tgatcttatt atcttgttca gacaaacaaa tagcaatgcg acaaaatgtc gcacctccat
       61 caacaaaaaa ccgacaagtt attgcatgca tgtcggtttt taaatcgcta tttacacgaa
      121 tgcttcattt tagaaaacac aaacttgaac tacagtaaaa aagtaagtag ctaaatcgta
      181 ctgtttcagt gatcaaatat taatttatgt aatacgattc tttgcctggg ttacaggcgg
      241 atttctaaag ggagacagct aatggagggc gttgtggctg ttcaggtata aatgaggtaa
      301 aaccgggatt aaatgaagta attgcagtag tgtcgttagt gggtatgggc gcacgatagc
      361 ccgacaaaat ctgggtatta ctgtatgtgt cacaagaagt atcgtcgcat ggcaggtttg
      421 ctagcaaatg gtcggttagc cctcagctta tgacagtcac catgatgatg attatcagct
      481 atcattgtct tatcatagtg cttgctaaaa tcttcctgca tacacactga cagaacggca
      541 gcagcaatgc cttgcagggg cagctccagc atggaaaaaa caatatccac ttggatttta
      601 aagtttccat ttttttaaac acattttagt gctcatgcga tgtctcgcct tttttcatct
      661 ccccgatcaa gtaataggca ttgttccatg ccactttagc gttcataggt agcggttcga
      721 ggaggttgat ttccacccaa ccttcactag aaatgcctgt tcttatttca acaggactga
      781 acacccattc catttttccg ctttcctggc gcgtgtgcgc cataaaaatg taaggcttac
      841 cttcctctat gatgatggct tcttccggta gagctttaac ggactcactt tctgtgtgga
      901 tttttccacg gatgtacatt cccggaataa gaaagtgttc cttttgatcg atctcggcgt
      961 gcacacgaac tgccataggt tttctctcaa aattcttttc aacagaaaag attatggctg
     1021 ataaattttt atccggaact gattgaaccg tgaatgatac tttttgacct ttttttactt
     1081 tatatatatc tttctcgaaa accattagat cagcatgtat ataatcatta tttataacac
     1141 cgaacattgg tgcttgggga tctataaact gccctgtctg tatcaatact ttttcgatat
     1201 agccatcaat agggcttact accggtacat attgaacaat attcccatcc ctgacctttc
     1261 tcacctcgat atttaattgc ttcaattgcg tttcaagacc ttttgcttct cccttgatgg
     1321 tttggtagtc agcttgcgtt tgttgatagg tctttcctga gcctatccct tcatcgtaaa
     1381 gccttttttg acggacatat tctttttcca gaaattgcat ctggttatag acttttatgt
     1441 atgcgatttg gaggtttgtt aggttagggt gtgaaaggta ggctagtacc tgactttttt
     1501 ttactttttc tccctcaatt actttgatat caattacatt ggctcctaaa atagccgtca
     1561 ctatggcttt atgctgggga aacaattcca atctgccgtt gacattcacc accccagaaa
     1621 gcgcacgtat aggtatcgta tctactttga tgttaagact attaaatttt tgcgttgaaa
     1681 gatgcactgc attcatttct tctgcgtcgt tatgtccttt gttggcgcta tcctcatgag
     1741 gatgctcatc atggccatgc tcttttttag catttccttt ctgagacgcc tgatttttac
     1801 catctcccaa attactgttg tcagccgatt tgctagtaca actggtaagt aaaattgcta
     1861 ttaagaccaa ggtaaatggg aataggatgt attttttcat cgttatttac tctttattta
     1921 tttgattttc gcgttagttt gaggttttta agaagtattc cagctggtag cgactatcca
     1981 gataattgcc aaaggcattc catgagtcga cttcaatacg aatcgcttct ctcatgtttt
     2041 gcagaaatgt tacatagtct atggcgcctt cctgataagc agtaactgct ccggtttgct
     2101 gctccctggc cataggaagc gccgtgtccc ggtagtattg ccatgagttc agccacttga
     2161 tgtactgctc acggatattc tgataagccg cagctagttc tagttgagat tgctgaagat
     2221 tctgattggc aattgtgtgt tcgatctttg cagcctgcga tcttcccagt tccggtgcaa
     2281 aaaataacgg tacttcaatg ccaacctgaa attgatgaag tccaggcaga ccagatattt
     2341 cttgtttgcc atactgtaaa cttaacttgg gtaaaaattg cgaacgcctc tctttgatag
     2401 cagcctctgc tacactaacc ttttgcttag aaacattaag tacaggatgg gcgtctatag
     2461 aatctgatat taagttcagt ggactttcca attgatttgc agggatatct gggacggtga
     2521 atacagtgtc agtgacaaac cataggttca atcgatgcag cgcacttaaa tagtcgcgat
     2581 aggcctgttc tttctgtatc tgcacttgat ttccctgatt cgaggttgcc aggtattcca
     2641 acttagaagt cgctttaact tcaagtctta tccgggctgc ccgttcaata tccatgaata
     2701 tcgagtccag ccgtttgtaa acctgataat tatttttagc ggtatacgcc atagcccaag
     2761 ctcggcttac ttcccgttct acttctatca cagacaggtt aagcgccttc tcagccaatg
     2821 ccacccgctc tttttgtagc tccagtttag gaacaatacc aaatatatta atattgctct
     2881 gtttgatacc aacattcata taagtagttt ctggggtata ccctatcttt tctgcaccag
     2941 tgaagattga ggtactacca aggtcccaag cggttttttt ctgaacttct tggttttcta
     3001 tttcaagtct tgctgcctgt atctttggaa aattctgtac agctaattcc ttagcttgct
     3061 ccagggtaat cgtttgcaga ctgtctattg gctgtgtttg tgcattcact gtagaggcag
     3121 tgccaagtag gatactaagc agcccaccgc atacaatcag tatgaacaat acatttgtat
     3181 tagggcgttt aaggcttata aggtttttct tattattact tcgattctcc acgaaagcgt
     3241 aaattaccgg aagcactata agagtaagca gggtacccga gatcaaacca ccaataacta
     3301 cagttgctaa ggggtgttgc acttcagcgc ctgctgacac agataatgcc atgggcaaga
     3361 agccgagtat atccgtagca tctgtaagta aaataggcct gattctttcc ttaactgcta
     3421 taagtatccg ctcctcaata tcgataattc cctcttcttt taatgaattg aatcgactaa
     3481 ttaatactaa gtcatcaagc acggctacac caaataacac gacaaaaccg actccggctg
     3541 aaacactaaa aggtatatcc cgtaaccaaa gagcgaatac cccgcctatg gccgctagag
     3601 gaatggcggt aaaaagcatg atcgactggg aaaacgactt cagggcaaaa tacagcaaaa
     3661 cgaaaatcaa aaaaatggta attggcacca cgatagctaa tcgatttttg gctttttgca
     3721 ggttttcaaa ctctccgccg tatataaggt aatagccagg cggcaaattc agctcagcac
     3781 ccaatttttg ttggatatct ttcactacgg attccatatc acgtccccta atgttcactc
     3841 ccacataggt tcttcggaat acattgtcgc gggatatttg catgggaccc agcacaaagt
     3901 tgatgtcggc caactccttg atcggaacct gattgccgtt aggcaaatcg atataaagat
     3961 tgcgtaaatc gtcaatactt ttctggtggg ttttatcaaa acgtatgacc atgtcaaagc
     4021 gtttttctcc ttcaaaaatc actccagcca cacccccagc aaatgccgat ctgacatatt
     4081 cattcaactt atcaatattc aatccatatt gagcaatctt ttcgcggtta tagcgcacgg
     4141 tcatttgggg caggccggca gtcctttctg ggttgacatc acccacgccg ggaatggtct
     4201 cgatgatttt agccatctca tttgcttttt gcgccagcac atcaagatca tcaccatata
     4261 acttcacggc cacatcttcc cggacacccg ttaatagctc attgaagcgc agctgaatcg
     4321 gttggctgaa aaccaggttg actcccgtca gctcgttatc aagcttttct tttattttct
     4381 caataagttc atcttttgac ttagcagaga tccatttacc cttgtctttt tccagaataa
     4441 tgaacatatc agcgatatcc atcggcatag gatctgtagg aatttctgcc acaccaatcc
     4501 tggctaaagc gatttttatt tccggaaaat tttccagcaa aatgttttct atttttatcg
     4561 aaacatcaat ggcctcgctg agactactac ccggcctgat aagtgcttgc atagcaatat
     4621 cgccttcctc taactgcggt ataaattcgc cacccaacct ggtaaatgta aagatggcag
     4681 cagccagtaa cacaatcgcc gcaatcatga caattgcttg gtgccgtagg gcacttttta
     4741 gtaatggtag ataaacaagc tggaagccat ttatgatctt ttctccgact ttctcaagcg
     4801 atctttctaa cttaccagac cagttctttt tgttctttag tggttttaaa aataaggctg
     4861 acatcgctgg gacatatgtc aggcaaagga taatggctcc tagcatggta aacccaaaag
     4921 tgtaggccat ggggtggaac attttacctt ctattccagt aaggaataga ataggaacaa
     4981 atacgatcag tataattagc tgaccgaaaa ttgcggtatt catcatagaa cttccagctt
     5041 ggtaggctag ttcatccatg acatcttgat caatatcgaa tttaccggcc ttaaattttt
     5101 tttgaatttc atatatggtg cgttctacta taatcactgc accatcaaca ataattccaa
     5161 agtcaatagc ccccaggctt atcagattag ccgatacgtt aaaaagcttc atcaagatag
     5221 cggcaaagag gaaggacaag agaatggtac tggcagtaac aatgccgcct ctaatgctac
     5281 ctagaatgat gactaaggca aaaattacaa ctagcgcacc ttctgtgagg ttctctctta
     5341 ctgtgctggt agtccgttct ataagcgcgc ttctgtcaag aaaaggtcta atctccaagc
     5401 cttctggcaa tgatttctga acttcagcta tgcgcttttg cacgttttgg attacagcat
     5461 tagaatttgc cccccttagc atcaaaatca tgccacctac agcttcgcgg ccatcctgcg
     5521 tgaaagcgcc atagcgcact tggtgaccaa aatgtatttt ttccactaca tctctgatta
     5581 agacgggtat gccatttacg tctttaatag caatatttcc tatatcatca agtgaacgta
     5641 ccagaccttc cacgcggatg aaattggcca tgttattttt ttcaatgtag gcacccccgg
     5701 tattgttatt attgcgccgc aaggcctcaa atatttcaga gatggaaaca ttgttggcat
     5761 tgagattttc agggttgatt gctatttcat actgcttaat ttttccgcca aaggcattta
     5821 ttccgactac gccttctacc aaggctaatt gtcgtcttac gatccaatcc tggatggtac
     5881 gcaactcagt gggagagtac gctgtttcat atcccggctt gggctgaagg gtatattggt
     5941 aaatttctcc cagccctgtt gtaatgggtc ccatagacgg actgccaaaa ttttcaggta
     6001 tttgttgtat tactacatta agtttttcct gcactagttg ccgtggcaga taggtgccca
     6061 tatcatcttc aaagacgatg gttactaccg aaaggccaaa gcgggaaatc gaacgaattt
     6121 ccgtaacacc gggaaggttg gccatagcca attccacagg aaaagtaaca aatcgctcga
     6181 tgtcttcagt agccagattt tgtgaaaccg tgattacctg tacctgatta ttggtgatat
     6241 cgggtaccgt accaaggttc acggtgaaca atgaatatat ccctgcgcct atcagagcta
     6301 gggtaagcaa acccacaatg agtttatttt ttatagaaaa ataaatgatg ctattaatca
     6361 tgcacctgac ctattattag ataaaattat ctgactctaa tgatcaatcc aagggtaatc
     6421 tccatagaat tgtcgtgatc aaacagtaag tctcggcaga ggtatagatg tgacttgctt
     6481 acctagttgg cgcctaatgg cagcatcgag ctggcattaa catagagaga aacgatgact
     6541 tgtgtgctcg gctatcacat ccatttgcct gctgtgatct gaatagctag ttatgtgctt
     6601 agcctaaaca tttgctaaat tttgaatact aactattaaa gcagcttgga cttacgtttt
     6661 aactgtgaat gattttacga ggagggcgaa acggtgaatc tataaaagat tctggaactt
     6721 gcgacagaat aagtttaaca ggaatctctt tacctgcgat tggtactaat aaggggaatt
     6781 ttgcaaaaaa aaagggttga tatgctgcgc tgagacatat gtgtgccgaa agatccaaat
     6841 tcttttcatc agaaagttcg ttagtcagat gatcttcctg cattgtttgt gctgctgaat
     6901 gtgtgagatt atgacggtca agctcatgtg caaatacttc tccatggaaa gcccacgcaa
     6961 gcgtatttgc gagcaaactg agcgacagta ctaacactat aatgttaggt aatttacaca
     7021 ttgtaacagc ataacatatc tgaaaataaa tgttattatt tctggtctta agaagttaaa
     7081 atacatgcct cgcgtaaatt atcattttac tagactagca gatatgtctg atagtttaga
     7141 gccaacagat ttaccattca atggcaacaa aactccaatc attaaaaatt aaccaattaa
     7201 agttcttttc ccgctattta tatacaacct tcagctctgg ggtcatcgga taaactctct
     7261 atcttattta agatt
//
LOCUS       DAORTI010000153         7166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11891_length_7166_cov_6.552102, whole genome shotgun sequence.
ACCESSION   DAORTI010000153 DAORTI010000000
VERSION     DAORTI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11891_length_7166_cov_6.552102"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..839)
                     /locus_tag="PLO70_14465"
     CDS             complement(72..839)
                     /locus_tag="PLO70_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75722.1"
                     /translation="MDNSTPDMPEKLVRYLDGELNEDEKKIIEQQLNSDSVLQQEYNS
                     LLAAREAIRHYGLQQQVDAVHQHMMGEFQVPVKKIGSKRRIIRNAVAIAASVLLLVAG
                     YAAFNFFTLSPEKVFNSNFRVFELTTVRSDGAGETAAEKAYREKNYKEVIRIHDAGED
                     HTRKGEFLCGVAALELKDNKKAITCFTEVLDQNKQASVLILNDETEYYLFLSYIRDRD
                     YNAALAIMEKIKNDPGHLYHKKVTSKLIRQVKLLRWR"
     gene            complement(832..1437)
                     /locus_tag="PLO70_14470"
     CDS             complement(832..1437)
                     /locus_tag="PLO70_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ75723.1"
                     /translation="MEVIRNFPDSEMLANLRTGQRMDETIKAIYRSHFEGLCWYVMNN
                     NGSRQDAEDVFQEVMVNFIELVQKDKFRGEASVKTFLFSLNRYTWLNELKRRGRALVR
                     EEKYERGQDRVELDTSHFIADREGKAEVLRVVGELGETCRKILLLFYYENLSMKEILE
                     ATDYETEQVVRNKKYKCLKQLEQMVNANPALKQTLKNLLHG"
     gene            1533..2039
                     /gene="hscB"
                     /locus_tag="PLO70_14475"
     CDS             1533..2039
                     /gene="hscB"
                     /locus_tag="PLO70_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HQZ75724.1"
                     /translation="MTYFELFDIPVQLKINVTSLSKKFFELSRKYHPDYFANENDEAQ
                     AAVLEKSALLNKAWKVFQNPDESIKYVLQLKGLLVEEEKYELPPDFLMEVLEINEALM
                     DTQESGIPKLESRISELQSEIYEPVKKIVEDYKDGITTEKELLQVKEYYYKKKYLDRI
                     RRQLAGMA"
     gene            2062..2145
                     /locus_tag="PLO70_14480"
     tRNA            2062..2145
                     /locus_tag="PLO70_14480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2096..2098,aa:Leu,seq:caa)
     gene            complement(2186..3166)
                     /locus_tag="PLO70_14485"
     CDS             complement(2186..3166)
                     /locus_tag="PLO70_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HQZ75725.1"
                     /translation="MAVQWNHKVFGVSEPGFEALSLEIFRYQYENNRVYNKYVKALGV
                     NPSGIHTITQIPFLPIRFFKSHPVQTGDFIPEAVFESSGTTGAVNSRHLVRDLSLYTE
                     SFTRSFTLFYGPVKDWCIIGLLPSYLERSNSSLVYMVNNLITESAHKKSGFYLDEYDQ
                     LYTVLNELERQEQKTLLIGVTFALLDFAEQFQPVLKNTVVMETGGMKGRRKEMIRPEL
                     HELLTKSFRITTVHSEYGMTELLSQAWSTGEGIFNTPPWMKILVRDEEDPLLVNETGA
                     GAINVIDLANVHSCSFIATDDAGKVFKNRSFEILGRMDGSDLRGCSLLMV"
     gene            3220..3675
                     /locus_tag="PLO70_14490"
     CDS             3220..3675
                     /locus_tag="PLO70_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75726.1"
                     /translation="MKYSLFVLIVLVVIATACNKDKFTTEPQVEVKSITPETVFNGNI
                     INLKAKYTDDEGDLDSAYVVYKWYNGATVVKADTFRYPYSILNLPSDLRRADIEVTFE
                     YNTNNNPDLVPLPGVSVRDTTATFGLILIDKARHRSNYSESQPIRMKKP"
     gene            3687..4532
                     /locus_tag="PLO70_14495"
     CDS             3687..4532
                     /locus_tag="PLO70_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HQZ75727.1"
                     /translation="MKALRTSWTFFIYSNLFIAVCAVLLTHQLYRLVLNSPVNPQYAG
                     FVFFSTLCSYSFHWYFSSTSVLPSARINWLNRFRNVHAIFFFLGLAGVLFFISYFLEF
                     WPWLLAAAAATFLYSAPKIPNKYLRLLRKVALGKTIFLAAVWTYVTTVLPIIISGQDW
                     TPAFSLFVISRYFLIYAICILFDLRDREDDKAAGIRSLITYLGTGSIKILFTISLLIF
                     AIATLLLNNYHFSSVQIMLLSVPGIILALLYNRASRDFSDMLYYFVLDGLLAFSALLT
                     LIDGI"
     gene            4563..5672
                     /locus_tag="PLO70_14500"
     CDS             4563..5672
                     /locus_tag="PLO70_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HQZ75728.1"
                     /translation="MAKSVKAKKSIITDKSFEFFRNYINNASPVGFETWGQKLWIEYL
                     KPYVDTHYVDPYGTAVGIINPDNKFKVVIEAHADEISWFVNYITAEGLIYLKRNGGVD
                     HQTAPASRVLIHGKKGPVKAVFGWPAIHTRIGNPDQKEPVPRTDNLWLDCGARNKKEV
                     EALGIHIGAVVTYQDGFDELANDNYVGRAFDNRIGGFMIAEVARLLKENKKKLPFGLY
                     VVNAVQEEIGLRGAEMIARRIKPDIAIVTDVTHDTTTPMISKNIEGDVSTGKGPSLSY
                     APAVHNKLLALVEKVAADKKIPVQWRALSRSTGTDTDSFAYSNDGCPSVLISIPLRYM
                     HTTVEMLHRNDIENTIKLMYETLLTLTPKTNLSYL"
     gene            5669..5836
                     /locus_tag="PLO70_14505"
     CDS             5669..5836
                     /locus_tag="PLO70_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75729.1"
                     /translation="MIAYLLYIDPGSGSYLVQVIAAAVLGVAFFFRNIKNYIKAFFFR
                     SDKKSKDNTPD"
     gene            5836..>7166
                     /locus_tag="PLO70_14510"
     CDS             5836..>7166
                     /locus_tag="PLO70_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75730.1"
                     /translation="MPASFKTHASSYRDPSGFIFKKEEVLYRQVNKIFKADFDLFINS
                     GCYQSFAGKGWLIPHETIPENFTNTDDWYLTLKPANIPFISYPYEWSFDMLKDAALLT
                     LNLLKESLQYGLILKDATPYNIQFLGSRPVFIDTLSFEKYEEQEPWIAYRQFCECFLS
                     PLLLSCYSKQPLQQLMLAYPEGIPLAITRSLLPWRSKFSLHTYLHIHLHAGIAEKSKG
                     EKGKKIIFTRRKLQNLISSLEGLVNNLTLKPRSTPWSAYYDEASQRKDYLLHKKEIIN
                     DWIDKTDNIKTAADLGSNDGEFSFLLANKNIQTIAGDFDPQCINNLYNRLKGKKTTYI
                     LPLIIDIANPSPAIGVNNEERTAFTSRLNADLTLALALLHHLAIGRNIPFELIAVFFQ
                     HVTHQLIIEFIPKEDEKIQGMLTGKKDIYTAYTVTDFEKAFTKYFKIVDTKA"
BASE COUNT         2111 a         1555 c         1357 g         2143 t
ORIGIN      
        1 tgtaactatt tgaaataaaa aaacctctcc cggaaccggg agaggttacc accattatca
       61 actcaaaatt tttaacgcca cctaagtagc ttaacctgcc ggatcagttt actggtaact
      121 tttttatgat aaagatgacc cgggtcattc tttatttttt ccattatagc tagggcagca
      181 ttataatccc ggtccctgat ataactgaga aagagataat attctgtttc atcattcaga
      241 ataagaactg atgcctgttt attctgatca agtacttccg taaaacaggt gatagctttc
      301 ttattatcct ttagttccag tgctgctaca ccacacagaa attctccctt ccgggtatgg
      361 tcttcaccgg catcatgtat cctgattact tctttatagt tcttttcacg gtaggctttc
      421 tccgccgcag tctcacctgc tccatcgctc ctcacagtag ttaattcaaa tacccggaag
      481 tttgaattaa aaactttttc tggcgataaa gtaaaaaaat tgaaggcagc ataaccggcc
      541 actaacaata acacactggc agctatagct acggcgttac gaataatgcg tctttttgaa
      601 ccaatttttt taaccggcac ctgaaattct cccatcatgt gctggtgtac tgcatcaacc
      661 tgttgctgta aaccataatg acgaattgct tcacgggcag ccagtaggct attatactcc
      721 tgctgcaata ctgaatcgga gtttaattgt tgctcaataa tttttttctc gtcctcattc
      781 aattctccat ccaggtatcg aacaagtttt tcaggcatat cgggtgtaga attatccatg
      841 caatagattt tttaaagttt gttttaatgc aggatttgcg tttaccatct gttccaattg
      901 cttaaggcac ttatattttt tattcctgac tacctgctcc gtttcataat cagttgcttc
      961 cagtatctcc ttcatcgaca ggttttcata ataaaataag agcaatatct tacgacaggt
     1021 ttctcccagt tcacctacca ctcttaatac ttcagccttt ccctccctgt cagctatgaa
     1081 atggctggta tccagttcta ccctgtcctg tcccctttca tatttttctt ctcgtaccaa
     1141 tgcccggccc cgtcttttta attcattcag ccaggtgtat cgattcaggg aaaataaaaa
     1201 tgtttttacc gaagcctctc ccctgaactt atccttctgt accaattcga taaagttgac
     1261 catcacctcc tggaatacat cctctgcgtc ctggcggctg ccattattgt tcataacata
     1321 ccagcacagc ccctcaaaat ggctgcggta gatcgccttg attgtttcat ccattctttg
     1381 accggtgcgg agattggcca gcatttccga atccgggaag ttcctgatta cctccatgct
     1441 taattcataa atttgtaatt gtaaagtcct gttttgtaac ctaaactaag gatatttttt
     1501 tacgataacg gaaagaaact ccataaacgg ccatgaccta ttttgaacta tttgacattc
     1561 cggtacaact gaagataaat gtgacttcct tgtccaaaaa attctttgaa ctcagcagaa
     1621 agtatcaccc tgattatttt gccaatgaga acgatgaagc ccaggcagct gtattagaaa
     1681 aatcagcgct gttaaataaa gcctggaaag tatttcagaa cccggatgag tccattaaat
     1741 atgtgctgca attgaaagga ttgctggtgg aagaggaaaa atatgaactc cccccggact
     1801 ttctcatgga agtattggag ataaacgaag cattaatgga tactcaggaa tcgggtatcc
     1861 cgaagctgga atccaggatc agtgaactgc aatcagaaat atatgaacct gttaaaaaaa
     1921 ttgtggaaga ctataaagac ggtattacta ctgaaaaaga attgctgcag gtaaaagagt
     1981 attactataa gaaaaaatat ttagaccgta ttcgccgcca attagccgga atggcttaat
     2041 attgcagccc aattcaaata agcccgggtg gcggaattgg tagacgcagc agactcaaaa
     2101 tctgccgttc gcaaggatgt gctggttcga ttccagtccc gggcacagtt taaaacggtt
     2161 cacaaatggt gaatcgtttt ttgctttata ccatcagcag gctgcaccct ctcaagtcgc
     2221 tgccatccat tctgcccaaa atctcaaaac tcctgttttt aaatactttc ccggcgtcat
     2281 cagttgcgat aaaactacag gaatgaacat tggccaggtc aataacattg atagccccgg
     2341 caccggtttc attaaccagc aatggatctt cttcatccct caccaatatc ttcatccagg
     2401 gtggcgtatt aaaaatccct tcccccgttg accaggcctg tgatagtaac tcagtcatgc
     2461 catattcaga gtgaacggtg gtgatacgaa atgacttcgt taataattca tgcaattcag
     2521 gtcgtatcat ttcttttctt cgacctttca tacctcctgt ttccattacc actgtatttt
     2581 tcaacactgg ttgaaattgc tctgcgaaat ccagtaatgc aaaagtgact cctatcaaca
     2641 gtgttttttg ttcctggcgt tccaactcat ttagaacagt atatagctga tcatactcat
     2701 ccaggtaaaa accacttttt ttatgagcac tttccgttat cagattattt accatatata
     2761 caagtgaaga attacttctt tccaggtatg atggtaataa tcctatgata caccagtctt
     2821 ttacagggcc ataaaaaaga gtaaagctcc tggtaaaact ttctgtatat aatgaaaggt
     2881 cccgaactaa atgacggcta ttaacagcac cggtagtacc actgctttca aaaactgctt
     2941 ccggaataaa atcgcctgtt tgtaccgggt gagatttaaa aaaacggatc ggtaaaaaag
     3001 gaatttgcgt aattgtatgt attccggagg ggtttacacc cagtgctttt acatacttat
     3061 tatacaccct gttattttca tactggtacc ggaaaatttc cagcgaaagc gcttcaaaac
     3121 caggttccga aaccccaaaa actttatgat tccattgtac ggccatacct gttttatgtc
     3181 ctcaaaaatt gtaaatttgt tcaaacctaa gatagtctca tgaaatacag tctttttgtg
     3241 ctcatcgttc ttgttgttat tgcgactgca tgtaacaaag ataagtttac cacagaaccg
     3301 caggttgaag taaaatctat aaccccggaa actgtcttta atggtaatat tataaacctg
     3361 aaagccaaat atacagatga cgagggtgac cttgattcag cttatgttgt ctacaaatgg
     3421 tataatggag ccacagtggt taaggccgac acctttcgtt atccttactc cattttaaac
     3481 ctgccctccg atctaagaag ggcagatatt gaagtgacat ttgaatataa taccaacaat
     3541 aacccggacc tggttccttt gccgggtgtt tcagtaagag atacaacagc cacttttgga
     3601 ttgatcctca tagataaagc ccgtcatcgc agtaattaca gcgagtcgca acctatcagg
     3661 atgaaaaaac cataagcggt tattcattga aagcactccg gacttcctgg acttttttca
     3721 tttatagcaa cctttttata gcggtttgtg cagtactatt gacccaccag ctatatcgcc
     3781 tggtacttaa cagccccgtc aatccacagt atgccgggtt tgtgttcttc tctacgcttt
     3841 gcagttatag ttttcattgg tatttttcat ctacctctgt cctgccatct gccaggatta
     3901 actggctgaa caggttcaga aatgtacatg ccattttctt tttcctgggg ttggccggtg
     3961 tgcttttctt tatttcctat tttcttgaat tttggccatg gttgttagca gctgctgcag
     4021 caactttttt gtattctgca ccaaaaatcc ccaataaata tcttcgcctg ctccggaaag
     4081 ttgcgctggg taaaaccatt tttttggccg cagtatggac atatgtcaca actgttttac
     4141 ccataatcat atccgggcag gattggacac ctgcattttc actctttgtt atcagccgtt
     4201 attttcttat ttatgccatt tgcatcttat tcgatttgcg tgacagggaa gatgataagg
     4261 cggcaggtat ccgaagcctt attacatatc tcggcaccgg tagcatcaaa attcttttta
     4321 ccatttcctt gctgattttc gcaattgcca cacttttgtt aaacaattat catttcagca
     4381 gcgtgcaaat aatgctatta tctgtcccgg gtataatcct ggcactgctg tacaacaggg
     4441 ccagcagaga tttttccgat atgctgtatt attttgtttt ggatgggctg ttggcctttt
     4501 cagcactact gactttaata gacggtattt aattaatttt gacaataatt aattctaaga
     4561 gcatggcaaa atcagttaaa gctaagaagt cgatcatcac agacaaatca tttgaatttt
     4621 ttcgtaatta tatcaataat gcgtcaccgg taggttttga aacctggggt cagaagttat
     4681 ggatagaata cctgaagcct tatgttgaca cgcattatgt agatccttat gggacggctg
     4741 ttggcataat caatccggac aataaattta aagtagtaat agaagcacat gccgacgaga
     4801 tcagctggtt tgtcaattat ataacagccg aagggctgat ttaccttaaa agaaatggag
     4861 gtgttgacca ccagacagcc cctgcctccc gtgtgttaat tcatggaaaa aagggtccgg
     4921 taaaagcagt ttttggctgg cctgctattc atacccgcat tggtaaccca gaccagaaag
     4981 aaccagttcc gagaactgat aatttatggc ttgactgtgg tgccaggaat aaaaaggaag
     5041 tggaagcact gggtatccat ataggtgcgg ttgttactta ccaggatggt tttgatgaac
     5101 tggccaatga caattacgtc ggtcgtgcat ttgataaccg tatcggcggc ttcatgatcg
     5161 cagaagtggc gagactactc aaagaaaata aaaagaaact tccatttgga ctctatgttg
     5221 tcaatgccgt acaggaagaa attggtttac ggggcgctga aatgattgcc cgcaggataa
     5281 agcccgatat tgccattgtg actgacgtga cacacgatac cactacacca atgatcagta
     5341 aaaatattga aggtgatgta agcactggta aagggccttc cctatcctat gctcctgctg
     5401 tacataataa actgctggca ctggtggaga aagtggctgc tgataaaaaa atacctgtgc
     5461 aatggcgggc attaagccgc agtaccggta ccgatactga ctcctttgct tattcaaacg
     5521 atggttgccc gtctgtattg atatcgattc cgctgcgcta catgcacact acggtggaaa
     5581 tgttacacag gaatgacata gagaatacca ttaaactgat gtatgaaaca ttgctcacct
     5641 taacgcccaa aacaaacctg agttacctgt gatagcttac ttattatata tcgatccggg
     5701 gagcggcagc tatttagtac aggtgattgc tgctgccgta ttgggtgtag cctttttttt
     5761 caggaacata aagaactata taaaagcatt tttctttcgc tccgataaaa aatcaaaaga
     5821 taatacaccg gactgatgcc agcctctttc aaaactcatg cttcttctta cagggatccg
     5881 tcaggattca tctttaagaa agaagaagta ctataccggc aggtaaataa aatatttaaa
     5941 gctgattttg accttttcat aaacagtggt tgttaccagt catttgcagg aaagggatgg
     6001 cttataccgc atgagaccat acctgaaaat tttaccaaca ctgatgactg gtatttaacg
     6061 ttaaaacccg caaatatccc tttcatatct tatccatatg aatggagttt tgatatgctg
     6121 aaagacgcag cattacttac cttaaactta ctgaaagagt cattacaata tggcttaata
     6181 ctcaaagatg ccacccccta taatattcag ttcctgggga gcaggcctgt gttcattgat
     6241 accctttctt tcgaaaaata tgaagagcag gagccgtgga tagcttaccg ccagttttgc
     6301 gaatgcttcc tttccccttt actgctttcc tgttacagta aacagccttt acagcaatta
     6361 atgctggctt acccggaagg gatcccattg gcaataacaa ggtctttatt accctggcga
     6421 agcaagtttt cacttcatac ctacttacac atacacctgc atgcaggtat tgcagaaaaa
     6481 agtaaagggg aaaagggaaa gaaaataata tttaccaggc gaaagttgca gaaccttatc
     6541 agcagcctgg aaggtctcgt caataatttg acgctaaaac cccgcagtac tccatggtca
     6601 gcatattacg atgaggcttc gcaaagaaaa gattatctcc tacacaaaaa agaaattatt
     6661 aacgactgga tagacaagac agataatata aaaactgctg cagaccttgg cagcaatgat
     6721 ggtgaatttt cctttttatt ggccaataaa aatattcaaa cgattgccgg tgattttgac
     6781 ccgcagtgta tcaataatct gtataacagg ctaaaaggca aaaaaacaac atatatactc
     6841 cccctgatta ttgacatagc caatccttca ccggctattg gtgtaaataa cgaagaaaga
     6901 acagccttta ccagccggct aaatgctgac cttactcttg ctttggcgct gctacaccac
     6961 cttgctattg gcagaaacat cccattcgaa ctaattgcag tattctttca gcatgttacc
     7021 catcaactca tcattgaatt catacctaaa gaggatgaaa aaatacaggg gatgctaaca
     7081 ggaaaaaaag atatttacac agcctacaca gtaactgatt ttgaaaaggc ctttacaaaa
     7141 tattttaaaa ttgttgacac aaaggc
//
LOCUS       DAORTI010000154         7146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_11937_length_7146_cov_5.581723, whole genome shotgun sequence.
ACCESSION   DAORTI010000154 DAORTI010000000
VERSION     DAORTI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11937_length_7146_cov_5.581723"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..1731)
                     /gene="rocD"
                     /locus_tag="PLO70_14515"
     CDS             complement(496..1731)
                     /gene="rocD"
                     /locus_tag="PLO70_14515"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HQZ75731.1"
                     /translation="MYSTISMTEKSLHYLELEEQYGAHNYHPIPVVLTRGKGVHVWDV
                     DGKQYYDFLSGYSAVNQGHCHPVIVKSFIEQVQKLTLTSRAFHSDLLGEYVKYITGYF
                     GYEKVLPMNTGVEAVETAIKLCRKWAYEVKGIEEGKATIIVCEGNFHGRTTTVISFSS
                     DPQAKKNFGPYMPGFTAIPFNDTEALAKALEDKNVAGFLVEPIQGEAGVLVPDDGYLA
                     KAKQLCEAAHVLFMADEIQTGLARTGKMLACDHENVRPDILILGKALSGGMMPVSAVL
                     ADDEIMMTIKPGEHGSTYGGNPLACKVAITSLQVLKEEKMAEHAAAMGELLRNELTAL
                     QSKHISLIRGKGLLNAIVIDHPHKDAAWELCLQLKENGLLAKPTHGDKIRFAPPLVIT
                     GSQIKDAVTIIGKSLAVLD"
     gene            complement(1910..2266)
                     /gene="queD"
                     /locus_tag="PLO70_14520"
     CDS             complement(1910..2266)
                     /gene="queD"
                     /locus_tag="PLO70_14520"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQZ75732.1"
                     /translation="MEIYKEFAFDAAHFLPNVPEGHKCKQMHGHTYHLRVYIKGIPEK
                     ELGWIMDFKELKDAVAPLIDQVDHQLINDVPGLENPTAENITIWFWNQLKPRLQALSR
                     IELKETPTTGVIYDGR"
     gene            complement(2324..3154)
                     /gene="ispE"
                     /locus_tag="PLO70_14525"
     CDS             complement(2324..3154)
                     /gene="ispE"
                     /locus_tag="PLO70_14525"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQZ75733.1"
                     /translation="MIVFPNCKINLGLNILRKRSDGYHDLETVFYPIALTDVLEIIEF
                     KNHGRTFSIPFSITGLPVKGEAASNLCVRAYKLLKKDFPHLPHIQVHLHKVVPTGAGL
                     GGGSADAAFTLQLLNDLFDLKISRKQLINYAAELGSDCPFFIINKPCFAKGRGEKLEE
                     LNVDLSAYKIIIINPGIHIDTGRAFLAITPALPAKSIKDIIAQPVSSWKDELVNDFEA
                     VIFPEHKEIAAIKTQLYEAGAIYASMSGSGSTVFGLFPASKELKLTLPDHYFVKLLPA
                     "
     gene            complement(3202..3807)
                     /locus_tag="PLO70_14530"
     CDS             complement(3202..3807)
                     /locus_tag="PLO70_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQZ75734.1"
                     /translation="MKKIELEIVALSHSITQTHSYAVVLGEVNGLRRLPIVIGGFEAQ
                     AIAVALEKMAPSRPLTHDLMKNFMNAFAVELHEIIICDLQEGIFYSKLVCSTENDTVE
                     IDSRTSDAIALAVRFGCPIYTYENILESAGILMEDTAATGKKKKVKQEVMVEDKPTGN
                     EDLKTMTLEELNNLLNEVLDNEDYIRAIAIRDEINSRQKKK"
     gene            complement(4001..4966)
                     /locus_tag="PLO70_14535"
     CDS             complement(4001..4966)
                     /locus_tag="PLO70_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQZ75735.1"
                     /translation="MSVLIFIDTAEGHVKKVSHEVLSYGAKVAEQLGTTAEAIVLGAV
                     SDDLASLGKYGVKKIHHVANDTLNNLDAQVYTKVIAQVAAACNAKLIIFSNNLNGKAI
                     APRLSVRMKAGLVAGAVALPDTSNGFVVRKNVFSGKAFANIAVSTDIKIIALNPNAFQ
                     VITGEGIAEVVPFNASVDAPKVKVTAVNKTSGEIVLTEAEIVVSAGRGLKGPENWGMV
                     EELAKTLHAATACSRPVADAHWRPHNEHVGQTGYAIAPNLYIAIGISGAIQHLAGVNR
                     SKVIVVINKDPEAPFFKAADYGIVGDAFEVIPKFTEAIRKLKGVA"
     gene            complement(5031..5774)
                     /locus_tag="PLO70_14540"
     CDS             complement(5031..5774)
                     /locus_tag="PLO70_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQZ75736.1"
                     /translation="MKILVCISKTPDTTAKIAFTDNNTKFDVNGVQWIINPYDEWYAL
                     VKAIELKEADASTTIHLVTVGTADAEPIIRKALALGGDEAIRVNADSHDSFYIASQIA
                     EVAKQGGYDLVLTGKETIDYNGSSIGGMVAELLDLPYISLATKFELAGGNATITREIE
                     GGEEVNEVALPAVVSAQKGMAEARIPNMRGIMAARTKPLKVTEPTAADPMTTVTGFGL
                     PPAKAGVKLVAADNVEELVRLLHEEAKAI"
     gene            5909..6220
                     /locus_tag="PLO70_14545"
     CDS             5909..6220
                     /locus_tag="PLO70_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ75737.1"
                     /translation="MDRIEKLKEFLKASPTDSFLQHALALEYIKLGNDEEARRLFEEL
                     LNREPGYVGSYYHLAKLLERNDHTDEAITVYERGMEEAKKAGDNHAFGELRGAYEELT
                     F"
     gene            6222..>7146
                     /gene="tilS"
                     /locus_tag="PLO70_14550"
     CDS             6222..>7146
                     /gene="tilS"
                     /locus_tag="PLO70_14550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQZ75738.1"
                     /translation="MNLLQQFQHFIKEQHLFSPKDKLLLAVSGGADSVVLCELCKQAG
                     YDFIIAHCNFQLRGEESERDEQFVSTLGDKYNAEIRVKRFDTTQYATENKISIQEAAR
                     ALRYEWFEELVNDELAKDPVHNSPSAVHLLTAHHKDDNIETLLMNFFRGTGLHGLTGI
                     PVLYGHIKRPLLAVSKEEILQFARENKLDFVEDSSNQSSKYTRNYFRNEIIPAISNVY
                     PQVKENLQDNIERFKEIEKLYRLTVDELKRKLCKRKGTEWQIPVKQLMGFNNRALIYE
                     IIRDYDFNEKQVEELIKLAASESGRYIESPVL"
BASE COUNT         1976 a         1491 c         1567 g         2112 t
ORIGIN      
        1 aaaagaaaat tgcgatagct aagaacaggg gtaaaatgac ctttttcacg gtgtacagat
       61 tagttttcag agtttagatt aaatagggtg aatctacggt acttactacg tgttttccct
      121 cgatccggac gtaaatgtag cagtggtttt tcgaacttgc aagtttttct acgaattatt
      181 tttactattt tctgacctcc gcgggatgat gataaaaatg gctgtgtaag caattggttt
      241 taagagaagt aaggcataaa aaagtaagac gcactgaaaa aataattttt ctaaagtatt
      301 gattttgatt acagaaacaa taaaaatgtt agataggaaa atcccggctg ccttaaaacc
      361 aggatgaata tcgaaaagaa tcgttgccat gactaaaaac cagacaaaac aataatgaat
      421 gcacagccgg tagatctatg ggtccggtaa aaaccggctt tttcgtaccg ggtttattaa
      481 aattgtttgg ttgggttaat ccagtacggc aaggcttttc ccaatgatcg ttacagcatc
      541 ttttatctgc gatccggtaa taacaagtgg aggtgcaaag cgtattttat ccccatgcgt
      601 tggttttgcc agtaacccat tttcttttaa ttgcaggcaa agttcccagg ccgcatcttt
      661 atggggatga tcaattacaa ttgcattcag caatccttta ccacggataa gtgaaatatg
      721 ttttgattgc agtgctgtta attcgttgcg caataattct cccatggcag cagcatgttc
      781 cgccatcttc tcctctttca aaacctgcag ggatgttatg gctactttac aggccaatgg
      841 gttgccccca tacgtagaac cgtgttcacc gggtttgatg gtcatcatta tttcatcatc
      901 agccagcacg gcgctgaccg gcatcatacc accgctcaag gctttaccca gaattaaaat
      961 atcaggccgt acattttcat gatcacaagc cagcatctta cctgttcttg ccaggccggt
     1021 ctgtatctca tcagccataa ataatacatg ggcagcttcg cagagttgtt ttgctttggc
     1081 gaggtagcca tcatcgggaa ccaatacacc agcttcgcct tgtataggtt ctaccaggaa
     1141 ccccgccaca tttttatctt ccagtgcttt ggccagggct tctgtatcat tgaaagggat
     1201 cgctgtaaag cccggcatat aaggaccaaa atttttcttt gcctgcggat cactgctgaa
     1261 agaaatgacg gtggtagtac ggccatgaaa atttccttcg cagacaatga tggtagcttt
     1321 accttcttca atgcctttca cttcataagc ccatttacgg catagcttga tggctgtttc
     1381 cacggcttcc acaccggtgt tcatgggcag gactttctca taaccaaaat aaccggtgat
     1441 atattttaca tattcaccaa gcaggtcact atgaaaggcc cggctggtaa gcgtaagttt
     1501 ttgtacctgt tctatgaaag acttaacaat gacggggtgg caatgcccct ggttaacggc
     1561 agaataacca ctgagaaaat cataatactg tttgccatcc acatcccata catgcactcc
     1621 tttgccacgg gttaatacaa ccgggatagg atgataattg tgtgcaccat attgttcttc
     1681 cagttcaagg tagtgcaggg acttttcagt cattgatata gtggagtaca tagttgataa
     1741 gttataagag tgaaaaaaag gaaagaaata cggaaacaac cgggttgtac cggtcatgaa
     1801 aaacttatcc tcaatgattg agaagatcga gattggaagt aagaaaacct cttgcggata
     1861 tgttgttagt gaacagcatt gcctgcaaga tacaaaagat cagtgaaagt caccggccat
     1921 catagataac accggtagta ggtgtttctt ttaattcgat tcggctgagg gcttgcagcc
     1981 tgggtttcag ctggttccag aaccagatcg taatattttc tgcagtggga ttttccagtc
     2041 ccggaacatc attgatgagt tgatgatcaa cctggtcaat cagcggcgcc actgcatctt
     2101 tcaattcttt gaaatccatg atccagccaa gttctttttc aggtatgcct ttaatgtata
     2161 cccggagatg ataagtatga ccatgcattt gtttgcattt atgtccttcc gggacattgg
     2221 ggagaaagtg tgctgcgtca aaagcgaatt ccttatatat ttccattgac caaatttaag
     2281 cagcaaatgt agaagcattt ggaaagtctg cgtatgagtg tggtcaggcc ggaagcagtt
     2341 ttacgaaata atgatcgggc agtgtcagct tcagttcttt gctggcagga aacaaaccaa
     2401 agacagtact gccgcttccg ctcatggaag cataaatagc gcctgcttca tagagctgtg
     2461 ttttgatagc ggctatctct ttatgttcag ggaagatgac agcttcaaag tcattgacca
     2521 attcatcctt ccaggaggag actggttgtg caataatatc ttttatggat ttggcaggaa
     2581 gggcaggggt gatagcaaga aatgcccggc cggtatctat atgaataccc gggttgatga
     2641 tgatgatttt ataagcagac aggtcaacat tcagttcctc cagtttttct cctcttcctt
     2701 ttgcaaagca gggtttgttg atgataaaaa aagggcaatc acttcctaat tctgcggcat
     2761 aattgatcag ctgcttacgg gagatcttca ggtcaaacag atcatttaaa agttgcaggg
     2821 taaatgctgc atctgcactg ccaccgccca ggccggcacc ggttggaact actttatgca
     2881 ggtgcacctg tatatgcggc aaatgaggaa agtctttctt taataattta taagctctta
     2941 cacaaagatt agaagctgct tcccctttta ctggcaatcc tgtaatgcta aatggaatgg
     3001 agaatgtgcg gccatggttt ttaaattcaa ttatttccag tacatctgtt aaggcaatcg
     3061 ggtagaaaac tgtttccagg tcatggtaac catcacttct tttgcgaagg atattaaggc
     3121 caagattgat cttgcaatta gggaaaacta tcataggcta aggggacgct gaggtattca
     3181 aaaaaggatc agtgctgtag ctcacttttt tttctggcga ctgtttattt cgtcacggat
     3241 ggcaatggca cggatataat cttcattatc cagcacttcg tttaaaagat tatttaattc
     3301 ttctaatgtc atggttttca gatcttcatt cccggtaggt ttatcctcta ccattacttc
     3361 ctgtttcact tttttctttt tcccggttgc tgcagtgtct tccatcagga tgcctgcact
     3421 ttccagtatg ttctcgtagg tatagatcgg gcagccaaaa cgaaccgcca gggcaatagc
     3481 atcggaggtc ctggaatcaa tttctacggt atcattctcg gtagagcaaa ccagctttga
     3541 ataaaaaata ccttcctgca gatcacagat aataatttca tgcaattcta ctgcaaaggc
     3601 attcataaaa ttcttcatga ggtcatgggt cagggggcgg ctgggggcca ttttttccag
     3661 ggctactgct atggcctggg cttcaaagcc accaataacg attggtaaac gccttaatcc
     3721 gtttacttca cccaaaacga ccgcataaga atgggtttgg gtgatgctgt gtgataaagc
     3781 cactatttcg agttctatct tcttcatacg aatgtcacac gaaactaagg tttttcgcca
     3841 aaataatcgg tgactaagtg ttgtttttaa tcctcttttc tcaatgatca gttgtgtgtt
     3901 atcgcaatta agttaattaa acaaaccagg tcgttttgat aaaaaaatag tccggcacta
     3961 tagccggacc atacgtattt taagatcact cagcaattga tcaggctacg cctttcaatt
     4021 tcctgatggc ttcagtaaat tttggtatta cttcaaaagc atcacccaca ataccataat
     4081 cagctgcttt aaagaaaggt gcttccgggt ctttgtttat taccacaatc actttacttc
     4141 tgtttacccc ggcaaggtgc tggatggctc cggaaatacc gatggcgata taaagattcg
     4201 gtgcaatcgc ataacctgtt tgtccaacat gttcattatg cggtctccag tgtgcatcag
     4261 caaccggccg gctgcaggca gtggcggcat gtaatgtttt agccaactct tctaccatgc
     4321 cccagttttc gggacctttt aatccacgtc cggcacttac cactatctct gcttcggtca
     4381 gcactatctc tccgctggtt ttattgacgg cagtaacttt tactttgggt gcatcaacgc
     4441 tggcattgaa cggcactact tcagcaattc cttcaccggt gatcacctgg aatgcattcg
     4501 ggttaagggc aatgatctta atatccgtac ttacggcaat attggcaaat gctttcccgg
     4561 aaaatacatt cttcctcacc acaaaaccgt tgcttgtatc aggtaatgca accgctccgg
     4621 caaccaaccc ggctttcatg cgaacagaaa gacggggagc aatggcttta ccgttaagat
     4681 tgttagaaaa gatgatcagt tttgcgttgc atgctgctgc cacctgtgca atcacttttg
     4741 tatatacctg agcatcgaga ttatttaatg tgtcattggc aacatgatga attttcttta
     4801 cgccatactt gcctaatgat gccaggtcat cactcactgc accaagcaca atggcttcag
     4861 cagtggtacc gagttgttcc gccaccttag cgccatagga caacacttca tgtgaaactt
     4921 tttttacatg gccttcagcc gtatctatga atattaaaac tgacataaaa tgaatttgaa
     4981 tagttgaaaa tgtctcaatt ggtttattga gttattgatt aatgaccttt ttatattgcc
     5041 ttggcttctt catgtaataa gcgaacaagt tcctctacat tatcagccgc aaccaacttc
     5101 actccggctt ttgctggtgg taaaccaaaa ccggttaccg ttgtcatcgg gtcggctgct
     5161 gtgggttctg ttactttcaa cggcttggta cgggctgcca tgatcccccg catattgggt
     5221 atcctcgctt ctgccattcc cttttgtgca ctcaccactg ccggaagagc tacttcattt
     5281 acttcttcgc caccttcaat ttcccgggtg atggttgcat ttccacctgc cagttcaaat
     5341 tttgtggcca gcgaaatata ggggaggtct aagagctcag ccaccattcc gccgatggaa
     5401 gagccgttgt aatcaatggt ttctttaccg gtcagtacca ggtcatatcc cccttgtttt
     5461 gccacttctg caatttgcga agcgatataa aaactatcat ggctatcggc atttacccgg
     5521 atagcttcat caccgccaag ggccagcgct tttcgtataa ttggctccgc atctgctgtg
     5581 cctacggtta ccaggtgaat ggtagtggaa gcatccgctt ctttcagttc tatggccttt
     5641 acaagggcat accattcatc gtaggggttg atgatccact gtacaccgtt cacatcgaat
     5701 ttcgtgttgt tatcggtgaa agctattttt gccgttgtgt ccggcgtttt actgatacaa
     5761 actaaaattt tcatattact cttgtttgtt attaccccgg ttcaggtttt ggggtgaatc
     5821 gttaaaaata gttgtttatg caactaaaac aaagataagt gatgcaaagg taaatccggg
     5881 caaatccggg taaaattttt atatcaacat ggatcggatt gaaaagttga aagaatttct
     5941 gaaggccagt cccacggaca gttttttgca acatgccctg gcgctggaat atattaagct
     6001 gggcaatgat gaagaggcga ggcgtttgtt tgaagaactc ttaaacaggg agccgggata
     6061 tgtggggagt tattatcacc tggccaagct gctggaaagg aatgatcata cggatgaagc
     6121 tattactgta tacgaaagag gaatggaaga agcaaagaag gcaggagata atcatgcgtt
     6181 cggagaattg aggggtgctt atgaggaact aactttttaa gatgaacctg ttacaacaat
     6241 ttcagcactt tattaaagag caacatcttt tttcaccaaa agataaactg ttgctggccg
     6301 tcagcggcgg agcagattct gttgtgttat gtgaactttg caaacaggca gggtatgatt
     6361 ttatcattgc tcattgtaat tttcaactga ggggagagga gagtgaaagg gatgaacaat
     6421 ttgtgagcac acttggcgat aaatataatg ctgagattag agtgaagcga tttgatacta
     6481 ctcaatatgc cactgaaaat aaaatttcta tccaggaagc agcaagggcc ctgcggtacg
     6541 aatggtttga ggaattagta aatgatgaac ttgcaaaaga tcctgttcat aattcaccct
     6601 ctgctgtaca cctgcttact gcccatcata aggatgataa tattgaaacc ctgctgatga
     6661 attttttcag gggtaccggg ttgcacggat tgacgggtat ccctgtttta tatggtcata
     6721 ttaaaagacc attgcttgcg gtttcaaaag aagaaatatt gcaatttgcc agggagaata
     6781 aacttgattt tgtggaggat tcttccaacc agtcttccaa atacaccagg aattatttcc
     6841 gaaatgaaat tatccctgct atcagcaatg tgtacccgca ggttaaagag aacctgcagg
     6901 ataatattga acgtttcaaa gagattgaaa agctataccg gttaaccgtg gatgaattaa
     6961 aaaggaaact ttgtaaacgt aaaggaactg aatggcagat cccggtgaag caattgatgg
     7021 gttttaataa cagggctctg atctatgaga tcatccggga ctatgacttt aatgagaaac
     7081 aagtagagga gctgatcaaa ctggccgcca gtgaatctgg ccggtatatt gagtcgcctg
     7141 ttttgc
//
LOCUS       DAORTI010000155         7093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_12078_length_7093_cov_6.812731, whole genome shotgun sequence.
ACCESSION   DAORTI010000155 DAORTI010000000
VERSION     DAORTI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12078_length_7093_cov_6.812731"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PLO70_14555"
     CDS             complement(<1..1676)
                     /locus_tag="PLO70_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ75739.1"
                     /translation="MKKVLFTAVFLFIASIGFSQSVKWLNSSGGSGSPQIRDLAVDQA
                     GFIYAVGSFNANMSVHGNAVNTQGGFDVFITKFDPNGNFIWIKTISGGSNDFGYEIET
                     DQQDNVYVSVFCQSSPLQVSDSLLSSSVSGILRFDSSGSFEAVLTTGMSVFNMKCYQG
                     SIYARSQVNTLFKFNLDGGLIWSKPISGSITPNNVILTSPETSLEITPHGNIIFSQNC
                     TGNYTYDGVTVNSGSGTFAIATLIDTNSNLIRNYAWGMNEGSTIRPRSAIVDANHNVY
                     MAVNFPSSYTNTFGSSTILHLLNGSAFHALLKFDSLAAPVWAYNISCANSTNNFFDLS
                     LDNNGNIAMLGMYTDITTLGSISFPTNSFNGNIFTATISPEGTLLTGMGLGTNNTTDR
                     ANDLELLADGAFALGGMTGTGVAPVKFGCINSVTPGFLVLKYNYETPPLPDVSFTQLR
                     EQRKVFFSSTVSNTSNVLWEFGDGATSTQRNPVHTYTTPGNFNVRLIGQNSCGADTTT
                     SQILYKGIQKILPGKIANSRFQIVIAKGGFPFATAQMVLKKGTHTILAEST"
     gene            1811..3820
                     /locus_tag="PLO70_14560"
     CDS             1811..3820
                     /locus_tag="PLO70_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ75740.1"
                     /translation="MTHTIRLLLLTFILLCTVSFYTTAQPMRKEKMDSLLSIIKKDNP
                     DSNTVKAYTEIQRYYFNNGQYDSARQYVLLSIPVARKLNEKKRLSRILYNAGIIYTNL
                     TRYDSARLFLDEGVALVTETGDTAVLVHTIFANALLSTYQSDYSGAVEQLMEAVRIIE
                     SSDNPGIRILLPQAYGKIGNNLIAENQFEKGIEYAKKALLIKNYPDEDRYSVMLHLDI
                     SDAYLKLNNTSLNKLHLDTAIQLNANINNVQVNTLVLNSEGHYYDIARDYQASLKAYL
                     KAYAIADSVNNEYLEAEVGANVARIYLKLGNTTAAEQYALESNTTAIKLKQYKVAAST
                     YNTLKEIAVQKQDFRKAYRYAELNKSYTDSASNIETKNNILNLEAKYKQEKNKKEIAS
                     LQLSNTEKELAVVKRNRLLLIGGAGSAILVLFLGMLYRNSRQKNIIAEKDKVLQEEQI
                     RFLERQQQVVSLQSMINGQETERTRIAKDLHDGLGGLFSTIKMLFSTLQHERKELQQD
                     PLFTKSYEMVNTASEEVRRVAHNMMPEVLIKLGLVQATQELCNSISAGKLLNVSMQSY
                     GMDKRLNSSTEVMLFRIMQELLNNIIKHSHATEAIIQFNRDGNRLSVTVEDNGRGFNL
                     SETDGKTHAGLSSVESRVSYLNGKLSIDSQQEIGTTVMMDFLINE"
     gene            3867..4499
                     /locus_tag="PLO70_14565"
     CDS             3867..4499
                     /locus_tag="PLO70_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75741.1"
                     /translation="MISILVIDDHPLVGDGISMMVKEITHLQILTVCKTGKEALVFLE
                     KTSPDVILLDISLPDIDGLELCTLIRKKNKQTKIIGLTSTNEAGIITQLLANGGNGYL
                     LKNMEREELITAIDEVMNGRIFLSKAANQKILEQFHSLKDALKNKPVLTRREKEILKL
                     LYEGLTGPQIAEKLFLSPLTVETHRKNLMQKLNVNSVQQLLKVAGENKLI"
     gene            complement(4571..5596)
                     /locus_tag="PLO70_14570"
     CDS             complement(4571..5596)
                     /locus_tag="PLO70_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75742.1"
                     /translation="MKVIKLNTRNFVLLFTAFFYNSILQAQVGIGTTNPDTSTILDIR
                     SSTKGVLIPRMTELQRSRITETADGLLVQDLHTGKTVTNRNNYGQWGLVDPVINQNVN
                     FTAFFTAGSVHVLNYDLGSNHQWITFPISGPLQGVCSADNVIIFITQTHVNAIFEDAN
                     KVVQLSSQSLSGGPVVDMGITTNVSSIAVLLKGNTAYRFRLSGATWSTTALPSTPANL
                     RVLKDRIIFTAGTSVFYADGNALLSQPISGSRAAIYANADAAIIHTTTNLYSFVPLAI
                     GGMDVQTNTLATDITNVYDNGQKNRIVVQSEDKLWVLRYDPSVGGQWSSINITGPVDK
                     VVAADKF"
     gene            complement(5815..6519)
                     /locus_tag="PLO70_14575"
     CDS             complement(5815..6519)
                     /locus_tag="PLO70_14575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQZ75743.1"
                     /translation="MALPFFYIPAYSAEQNKIVLDEDTSKHVVQVLRMKTGEQLNLTD
                     GKGSLLTCEITDAHKKHCTVEVRAKTESPKPLKQVSIAISLLKNTNRFEWFLEKATEI
                     GVSEIIPLICERTEKEKFRYDRMQGIVISAMLQSQQCWLPVLQDPVEFNKLVNESSVK
                     QKFIAHCDEASVKNNLLIFQPFNSSVILIGPEGDFSPAEIELALQNNFTPVSLGGTRL
                     RTETAGVVAATLLCQA"
     gene            complement(6602..>7093)
                     /locus_tag="PLO70_14580"
     CDS             complement(6602..>7093)
                     /locus_tag="PLO70_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75744.1"
                     /translation="HINTSASEDAFRVQVNNSTKIRVWNNGGTSIGIASTPPVNGLLV
                     NGPIQPQNGIATPAKMVIESSADSIIVNAGGSQIIIAANGNILIKTTAPGSKIEIESA
                     GELKLTGNTINVTATSTLALNGALVRFNGGSTPVAKLGSVSTVSGPAATVTSNVSPTV
                     LTQ"
BASE COUNT         2244 a         1493 c         1443 g         1913 t
ORIGIN      
        1 gttgattctg caagtatggt atgcgtacct tttttaagaa ccatttgtgc agtagcaaaa
       61 ggaaagccgc cctttgcgat cactatctga aatcggctgt ttgcaatctt ccctggcagt
      121 attttctgta tgcctttgta caatatctgt gatgtagttg tatctgctcc acaactattt
      181 tgaccaatta gacgaacatt aaaattccct ggcgtagtat aagtgtgcac aggatttcgt
      241 tgtgtgctgg tagcaccgtc tccaaattcc cataaaacat tactggtatt actaaccgtg
      301 gaagaaaaaa agactttccg ttgttcacgg agctgcgtaa aacttacatc gggcaaaggc
      361 ggtgtttcat agttgtattt caacacgagg aaaccaggcg ttaccgaatt gatacatcca
      421 aactttacgg gagcgacgcc ggttccagtc atccctccta aagcaaaagc tccatcagcc
      481 agtaactcca gatcatttgc cctgtctgtt gtattattgg tgcctaaacc catgcctgtt
      541 aaaagtgttc cttcgggaga aatcgtggcg gtaaaaatat ttccgttaaa gctgttagtg
      601 ggaaaactaa tagaaccaag tgtagttata tcagtataca tacccagcat agcaatattt
      661 ccattgttgt caagtgaaag atcaaaaaag ttgttggtac tgttggcgca gctgatatta
      721 tacgcccaaa caggtgcagc cagtgaatca aacttcaaga gagcatgaaa agcagaacca
      781 ttgagtaagt gtaaaatggt actgctgcca aaagtgttcg tatagctgga aggaaaatta
      841 accgccatgt aaacattatg attggcatcc acgatcgctg acctcggccg gatagtagac
      901 ccttcattca ttccccaggc ataatttcgt ataagatttg agttggtatc aatcaaagtt
      961 gcaattgcaa aagtaccgct tccgctattg accgttacgc catcataggt ataatttccg
     1021 gtgcagttct gtgagaatat aatatttcca tgcggagtta tctccaggct tgtttctgga
     1081 gatgttagaa ttacattgtt gggggtaata ctccctgaga ttggcttact ccagataaga
     1141 ccaccatcta aattaaattt aaaaagtgta ttcacttgac tgcgggcata gattgagccc
     1201 tgatagcatt tcatattgaa tacactcatg cctgttgtca atactgcttc aaaacttccg
     1261 gaagaatcaa aacgcagtat gcctgatacc gaagaagaca ataatgaatc agatacctgc
     1321 aaaggtgaac tttggcagaa aacagaaaca tacacattat cctgctgatc tgtttcaatt
     1381 tcataaccaa aatcattact gccgccactg atagtcttta tccatataaa gttcccatta
     1441 ggatcaaatt tagttataaa gacgtcaaac cctccctggg tattaacagc atttccatgg
     1501 acagacatat tcgcattaaa cgatcccacg gcatatataa accctgcctg atcaaccgct
     1561 aaatcccgaa tttgcgggct accagagcca cctgatgaat tgagccattt tacagattgt
     1621 gaaaaaccta ttgaggcaat aaacagaaaa acagcagtaa ataaaacctt tttcatcagt
     1681 atataattta tataaaaatc cggtttacaa tgctgctggc caatacccgt aattcatcaa
     1741 ttttaagtac taaaaaatac ctgaatggag gtagatacaa tgacgtatta aattgtaatt
     1801 tgaaaaaatt atgacccata caatacggct gctattgctg acttttattc tgctttgcac
     1861 agtaagcttt tataccactg ctcaacccat gagaaaggaa aagatggaca gcctgctttc
     1921 catcataaaa aaagataacc ctgatagcaa taccgtaaaa gcctatactg aaattcaacg
     1981 atattatttc aacaacggac aatatgactc cgcccgccaa tatgtattat tatccatccc
     2041 ggtggccagg aaactaaatg agaaaaaaag attaagcagg atattataca atgcgggtat
     2101 tatctatact aacctcaccc ggtatgacag tgcccgttta tttctcgacg aaggagtagc
     2161 ccttgtaaca gagacagggg atacggcagt actggtacac actatttttg ccaatgcact
     2221 gctctccact tatcaatctg attattccgg cgcagtggaa cagctcatgg aggcagttcg
     2281 tatcattgaa agttctgata acccggggat acgaatttta cttccacagg catatggcaa
     2341 aatcggtaat aatcttatag ccgagaacca atttgaaaaa gggattgaat acgcaaaaaa
     2401 agcattattg attaagaatt atcctgacga agaccgctat tccgtaatgc ttcacctgga
     2461 catttcagat gcctatctta agctcaacaa taccagcctt aacaaacttc atcttgacac
     2521 ggccattcag ctaaatgcca acataaacaa cgtacaggta aacacccttg ttctgaattc
     2581 tgaaggccat tattacgata tagccaggga ttaccaggct tctttaaaag cctacctgaa
     2641 agcctatgca attgcagaca gtgtaaataa cgaatacctg gaagcggaag tcggtgctaa
     2701 tgtagcccga atctatctta aactgggtaa tacaacagct gcagaacaat atgcgctgga
     2761 aagcaatacc actgcaataa aactaaaaca atataaagtg gccgccagta cttataatac
     2821 actaaaggaa atagcagtgc agaaacaaga tttcagaaaa gcataccggt atgcagagct
     2881 gaataagtca tacacagaca gtgcctcgaa tatcgaaaca aagaataata ttcttaacct
     2941 ggaagcgaag tacaaacagg aaaaaaataa aaaagaaata gcttccctgc agctctctaa
     3001 tacagaaaag gaactggcag tagtaaaacg taaccggcta ttgctgatcg gtggtgccgg
     3061 ctcagcaatc ctggtattat ttttaggaat gctgtacagg aacagccggc aaaaaaatat
     3121 cattgccgaa aaagataagg tcctgcagga ggaacaaata agatttttgg agcggcaaca
     3181 gcaggtagtt tccctgcaat ccatgatcaa cgggcaggaa acagaacgaa cccggatcgc
     3241 aaaagacctg catgacggac tgggcggttt attctccacc ataaaaatgc ttttcagtac
     3301 cctgcagcat gaaagaaaag aattacaaca ggacccgttg tttaccaaaa gttatgagat
     3361 ggtgaatact gcctctgaag aagtccgccg ggttgcacac aatatgatgc cggaagtact
     3421 gatcaaactc ggactggtgc aggccacaca ggaactctgc aacagtatca gcgcaggaaa
     3481 actcctgaat gtatcgatgc agtcctatgg gatggacaaa aggctgaact cttccactga
     3541 agtaatgtta ttccgtatca tgcaggagct gctcaataat atcattaagc attcccacgc
     3601 caccgaagcc attatccagt tcaaccggga tggcaaccgg ttgagtgtaa ccgtagaaga
     3661 taacggccgt ggtttcaacc tctcagaaac agatggaaaa acgcatgccg ggcttagttc
     3721 ggtagaaagc agggttagct acctgaacgg gaagctatcc attgattcac aacaagaaat
     3781 aggcaccacg gtaatgatgg attttttaat taatgaataa actaagccgg cttttgtatt
     3841 ttcaataata aacagatttc gtttttatga tttcaatcct tgtaatcgat gatcatccgc
     3901 tggttggcga tggcatttcg atgatggtaa aagagataac acacctgcag attctaacgg
     3961 tctgtaaaac aggaaaagaa gcattggttt tcctggaaaa aaccagcccg gatgttattt
     4021 tattggacat cagcctcccg gatattgacg gactggaact gtgcaccctg atccggaaaa
     4081 agaataaaca aacaaagatc atcgggctta cctctaccaa tgaagctggc attataaccc
     4141 aactgctggc taatggcggc aatggttacc tgctgaaaaa tatggaacgg gaagaactga
     4201 tcactgccat tgatgaagta atgaacggaa gaatcttcct gagcaaagct gccaaccaga
     4261 aaatactgga gcaattccat tccctgaagg atgctttgaa aaataagccc gtactgaccc
     4321 ggcgggaaaa agaaattctg aaactccttt atgaggggct gaccggacca caaattgcag
     4381 agaaattatt tctcagccca ttaacggtag aaacgcaccg taaaaacctg atgcaaaaac
     4441 taaatgtaaa ttctgttcag caattactga aagtagccgg ggagaataaa ttgatatagt
     4501 acttttagaa ataatcccgg tcgttatact tttatcagat gcgtacaaag ttgcgtttgg
     4561 caacaccgct ttaaaattta tcggcagcca ccactttatc tacaggcccg gtaatattta
     4621 ttgaagacca ttggccaccg acacttgggt cataccttaa cacccataat ttatcttcgg
     4681 actgaacaac aattctgttc ttttgtccat tatcatacac atttgtaata tccgttgcca
     4741 atgtatttgt ttgtacatcc atacccccaa tagcaagcgg tacaaatgaa tatagattag
     4801 tggttgtatg tataatcgcc gcatctgcat ttgcataaat ggcagctcgg ctacccgaaa
     4861 ttggctggga cagcaaagcg tttccatccg cataaaatac tgatgtacct gcggtaaaaa
     4921 tgatacgatc ttttaacacc ctcaggtttg caggtgttga tggtaaagct gtggtagacc
     4981 aggtggctcc cgacagcctg aacctgtaag cggtattacc ttttagaagt acagcaatac
     5041 tagaaacatt tgtagttatt cccatatcca caaccggacc gcctgaaagt gactgggagc
     5101 tgagttgaac aactttattg gcgtcttcaa agatggcatt tacatgcgtt tgtgttataa
     5161 aaatgataac attatcagca ctacagacac cctgcaatgg gccagatata ggaaaagtga
     5221 tccattggtg gtttgatcca agatcataat taagaacatg tactgaccct gctgtaaaga
     5281 aagcagtgaa gtttacgttt tgattaataa ccggatcaac aagaccccat tgaccgtaat
     5341 tatttctgtt ggttacggtt ttaccggtat gtaagtcctg caccaacagt ccatctgccg
     5401 tctcagttat tctgcttctt tgaagctctg tcatccgggg tatcaacact ccttttgtgg
     5461 aagatcgtat atccagtata gttgacgtat cgggatttgt tgttccaatt ccgacctggg
     5521 cttgcaaaat actattataa aagaaagcag tgaagagtaa cacgaaattc cgggtgttta
     5581 actttataac tttcatggtg gtgattttag tttttataat gcagtgaaac gggaaaaaaa
     5641 ttacgggctg ttcttttcga tgaaacaaaa aagactacag aggtcatcct gctaaactaa
     5701 agatgaataa aagagggtat aaaataaata cctgcaatga atgaatttac cagctaaaaa
     5761 actacctgaa ttcaggtata aaccaggacg tttcgacgga gaaataaaga gatatcaggc
     5821 ctggcacagt aaagttgctg ccactacccc agccgtttca gtacgtaacc ttgttccacc
     5881 taaactgaca ggcgtaaaat tgttttgtaa ggcaagctcg atttctgcag gagaaaaatc
     5941 tccttcagga ccgatgagaa tgactgaaga gttgaatggt tgaaaaatga gaagattatt
     6001 tttcacagaa gcttcatcac aatgggctat aaacttttgt ttcactgatg attcgtttac
     6061 caatttatta aattcaaccg gatcctgcaa aacaggaagc cagcactgct gactttgcag
     6121 catggcactg atcacaatcc cttgcatccg gtcataccgg aatttctctt tctctgttcg
     6181 ctcacagatc agtggaataa tttcactcac gccgatttcc gttgcttttt ccaaaaacca
     6241 ttcaaaacga ttggtatttt taagcaagga tatggcgatt gatacttgtt taaggggttt
     6301 ggggctttcg gttttagctc ttacttctac agtgcaatgt ttcttatgag catcggtaat
     6361 ttcacaagtc agtaaacttc ccttgccgtc agtcaggttc agttgctccc ctgttttcat
     6421 ccgcagcacc tgcaccacat gtttagacgt atcctcatca agtacaatct tattttgctc
     6481 cgctgaataa gcaggtatat agaaaaaagg aagtgccatc ccggtaaaaa taatgaaata
     6541 ctgattacag gtgtactgca aatgacaacc agatttgccc tggtaaagta agtagtttaa
     6601 gctactgtgt caaaacggtt ggacttacat tactggtaac agttgcagcc ggcccgctga
     6661 cagtggacac acttcccaat tttgcaacgg gagtggagcc accgttgaaa cgaaccagtg
     6721 ccccattcaa agcgagggtg gaggtagccg ttacattaat tgtattacca gtcagtttta
     6781 attcaccggc cgactcaatt tcaatttttg aaccgggggc cgttgttttg atcaggatat
     6841 ttccatttgc ggcaatgatg atctggctgc cgcctgcatt tacaataata ctgtcagcag
     6901 aactttcaat gaccatttta gcaggggtgg caattccatt ctgtggctga atgggaccat
     6961 tgaccagcaa tccattgacc gggggtgttg aagcaatacc aatagaggta cccccgttat
     7021 tccataccct gatttttgta ctgttgttga cctgcaccct gaaagcatct tctgaggcag
     7081 atgtattaat atg
//
LOCUS       DAORTI010000156         7034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_12239_length_7034_cov_5.498639, whole genome shotgun sequence.
ACCESSION   DAORTI010000156 DAORTI010000000
VERSION     DAORTI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12239_length_7034_cov_5.498639"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PLO70_14585"
     CDS             <1..1468
                     /locus_tag="PLO70_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQZ75745.1"
                     /translation="VAIPSFLKYMLIAQGEHPFYLNTSKDKRANYSVELKKKDSYGST
                     TGSEQINITCGVTDFRWAMKNVPPLKEEAYTTTLLNYMAKLEFQLAAYLEPLSPQTVM
                     STWPDLAQSLLKRDDFGMQMENAATWMPGVLPAILQNATTETEKAKKIYAYLRDNVTC
                     TDYRQLYTESTLRNVFDKKSGGVAEINLLLTAMLRYAGIRADPVILSRRDYGFVYDNY
                     PVIGQFNYVISRAGIDGKEILLDASYPNLGFGKLNYDCYNGTAKVMNEQATHLSLKAD
                     QLTEKGNITVILSGNEKGEWKANINKLYGYYESVDVRKKLKANDRETWMKELVKEYDT
                     EVKLENLRIDSLTALEEPIAFNYDISYGGDQANIVYLNPVITGRHRINPFKSTDRLYP
                     VEMPYKILENYSIIIIVPEGYSADEMPKPLSLKMNEQGDASFSYSVSKPSANLISIQC
                     RLEINKTLFQTSEYEALREFFNRVVAKQNEQIVLKKTK"
     gene            complement(1482..1820)
                     /locus_tag="PLO70_14590"
     CDS             complement(1482..1820)
                     /locus_tag="PLO70_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75746.1"
                     /translation="MYEHRKQPLATKATFYQRVLKNIFIASLIMLLCILIGVAGYHYW
                     GEASWIDSLHNASMILSGMGPVIEIHTVSGKLFSSIYALFSGVVFITNVGIILAPAMH
                     RMFHRLHLEE"
     gene            complement(1901..2743)
                     /locus_tag="PLO70_14595"
     CDS             complement(1901..2743)
                     /locus_tag="PLO70_14595"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HQZ75747.1"
                     /translation="MLKITLSALSLSLLGITTGFSQPTKIDIQKGQKYQVETTNKTTS
                     SAEVMGQTMETLIDSKSISLIEVMNNNPEGTDLQATATKVLLTSSMMGQDMSFDSDKQ
                     DNEGPMAEGLSKLLNKPRAISIDNKGTITKQEVIESDMQTILTGNNAELSKTTELFIP
                     SLIGKELKAGDSINDIASLAKEKYSSRDSGTYRITKIENGVASITYTGTQYITTVMEQ
                     MGMEMNMIINSTVKTELQVDMKTGIVLLKSSELDTNTTVDAGGMTIPATGKTTTTIKI
                     TPAS"
     gene            complement(2796..3845)
                     /locus_tag="PLO70_14600"
     CDS             complement(2796..3845)
                     /locus_tag="PLO70_14600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ75748.1"
                     /translation="MSYKVSSICLKVLSVAAFLLFLQPVSAQYNWTELETELQAKQKL
                     FGTNAVAMIWKGDSLVYKKEMGDFTMKTQAPIASCSKWLTAALVMQFVDEGKISLDDK
                     VVKYIPEFDKYFKGYITIRHCLSHMTGIEDEGGLLKKLTQRKKFATLEEEVNAFAARE
                     IRANTGTDFWYGNIGLNIAGRVLEVVSKRKFDVLIRTKLLYPLGMRKTSFTNIDGGPV
                     NPSGGAKSTAEDYMKFLVMLLNKGKYNGVQILSENSVNEMMKVQNKPEQIKYAPKSAS
                     GFNYTTGSWAIEEKNGIATALASPGLFGTWPMIDYCRGYAYLIFVKNLLGEEKADAHR
                     ELKKTIDTQISAACQ"
     gene            4004..6613
                     /gene="clpB"
                     /locus_tag="PLO70_14605"
     CDS             4004..6613
                     /gene="clpB"
                     /locus_tag="PLO70_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQZ75749.1"
                     /translation="MNLGNFTIKAAEAFQQAQQIAFNAQSPNIETEHILKALLDQQDS
                     PVEYLLKKNNVTVNLVETKLDELISKLPKASGEAAQQISREANSVVLRAGAVLKQFND
                     EFVTTEHLLLAIVQGNDSTAKLLKNTGLTEKGLIAAIKDLRKGETVTSQTQSQEFNAL
                     NKYAKNLNELARQGKLDPVIGRDEEIRRTLHILSRRTKNNPILVGEPGVGKTAIAEGI
                     AHRIVNGDVPENLKSKIIYALDMGLLIAGAKYKGEFEERLKGVVKEVSSSDGEIILFI
                     DEIHTLVGAGGGEGAMDAANILKPALAKGDLRAVGATTLNEYQKFFEKDKALERRFQK
                     VMVDEPSVEDAISILRGLKDRYETHHHVRIKDEAIIAAVELSSRYVTDRFLPDKAIDL
                     IDESAAKLRLEMNSMPEELDTLERQIRQLEIEREAIKRENDELKLKELNTEIANLAVS
                     RDTYKAKWNQEKELVDNVQNAKEEAERLKLSAEQAERNGDYGLVAEIRYGKIKEQEKL
                     IADLTRQIEESGEKRLLKEEVDAEDIAESIAKATGIPVQKMLQSDREKLLKLEEHLHE
                     RVVGQDEAITAVADAIRRSRAGLQDPKKPIGSFIFLGTTGVGKTELAKALAEYLFDDE
                     SMMTRIDMSEYQEKHTVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHP
                     DVWNVLLQVLDDGRLTDNKGRVVNFKNTIIIMTSNIGSHLIQDAFDGVNENNIEEANA
                     KAKLDVMNLLRQTIRPEFLNRVDEIIMFQPLLKKEIAGIVKIQLNNLQKLVADSGIQL
                     VFSDYALEFLADQGFDIQFGARPLKRLIQKEIVNQLSKRILAGDIDKTKPVLVDVFDG
                     VVVFRNETPEKVK"
     gene            complement(6654..>7034)
                     /locus_tag="PLO70_14610"
     CDS             complement(6654..>7034)
                     /locus_tag="PLO70_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75750.1"
                     /translation="DNQVLTVIVFLMSFVLIGGVNLWLTINYYNHSKGMILQLSQGAK
                     MFFFGNEANLKKFNKVDIHEIIIDRNTASKCPWNNFSLTTIYFKDGTHLKITSIMLDG
                     WDIKQKFPGIKITENSPFIPFYTT"
BASE COUNT         2108 a         1416 c         1603 g         1907 t
ORIGIN      
        1 cgtcgccata ccgtccttcc ttaagtatat gctgatagca cagggagaac acccttttta
       61 tttgaacacc agcaaagata aacgggctaa ttattccgtg gaattaaaga aaaaggattc
      121 atacggatca acaactggat cggaacagat caatattacc tgtggtgtaa cagattttcg
      181 ctgggcaatg aaaaatgtgc ctcctttaaa ggaagaagcg tatacgacca ctttgttgaa
      241 ttatatggca aaactggagt tccagttggc tgcttacctt gaacctttaa gtccacagac
      301 tgtaatgtcc acctggccgg atctggccca aagcttatta aaacgggatg attttggtat
      361 gcaaatggaa aatgccgcca cctggatgcc gggagtattg ccggccattt tgcaaaatgc
      421 cacaacagaa acagaaaaag cgaagaagat atatgcctac ttacgggata atgttacctg
      481 taccgattat cgccagcttt atacagaaag taccttgcgg aatgtatttg acaaaaagag
      541 cgggggagta gccgagatca atcttttatt aacggccatg ctgcgttatg ctggtatccg
      601 ggcagacccc gttatcctca gccggagaga ttatggattt gtctatgata actaccctgt
      661 cattggccag ttcaattatg ttataagccg ggccgggatc gatggaaaag aaatactgct
      721 ggatgcaagc tatcccaatc tcggctttgg caagcttaat tacgattgct ataatggaac
      781 agcaaaagtg atgaatgagc aagccaccca tctttcgctt aaagctgacc agttgacgga
      841 aaaaggaaat attacggtaa tcctttccgg caatgaaaaa ggggaatgga aggcgaacat
      901 aaacaagttg tatggctatt acgaatcggt tgatgtaaga aaaaagctga aagcaaatga
      961 cagggaaact tggatgaaag aacttgtcaa agaatatgac accgaagtga agttagaaaa
     1021 cctgcggatt gattcactta cagccctgga agaacccatt gcttttaatt atgatatttc
     1081 ctatggcggg gatcaggcca atatcgtcta tcttaatccc gtcatcacag gcagacacag
     1141 gatcaatccc ttcaaatcaa ctgacaggct ttacccggtg gaaatgccct ataaaattct
     1201 tgagaattat tctatcatca taatagtgcc cgaaggatac tcggctgatg aaatgccgaa
     1261 accacttagt ctgaaaatga atgagcaggg tgatgcttca ttctcttatt cggtctcgaa
     1321 accatcagcg aatctcattt cgatacaatg tcggctggag atcaataaaa cgctttttca
     1381 aacgtcagaa tacgaagcct tacgggaatt cttcaaccgg gtggtggcca aacaaaacga
     1441 acagatcgtt ttaaagaaaa ctaaataggc cgttctcttt tttattcttc taggtgcaaa
     1501 cgatggaaca tgcggtgcat agccggcgct agtatgatgc ccacattggt aatgaatacc
     1561 actccgctga atagcgcata aatggaagaa aataatttgc cgctcactgt atgtatttca
     1621 attactggcc ccatgccact cagaatcatg gatgcattgt gcagtgaatc tatccaggaa
     1681 gcttctcccc agtaatgata tccggccaca ccaattagaa tgcaaagcag cattataagc
     1741 gatgcaataa agatattttt taatacccgc tggtaaaaag tggctttggt ggccagcggt
     1801 tgttttcggt gctcatacat aaggctgctt taatattgct aaaggtacca aaccccgggt
     1861 agcttcggga aaacctgatg taatcgaact gcttctgcga tcatgaagcc ggtgtgatct
     1921 ttatcgttgt tgttgtttta ccggttgccg gtatcgtcat ccccccggca tcaacggtgg
     1981 tgttggtgtc cagttctgat gattttaaca gtacgattcc tgttttcatg tctacctgca
     2041 attcagtttt cacggtacta ttgatgatca tattcatctc catacccatt tgttccatta
     2101 cggtagtgat atactgtgtg ccggtatacg ttatgctggc cactccgttt tcaatttttg
     2161 tgatcctata agtaccggaa tcccttgaac tgtatttttc ttttgccagg gaggcaatat
     2221 cattaatgct gtcaccggct ttcagttctt tgccgattaa tgaaggaata aataattcgg
     2281 ttgtttttga gagttcagca ttatttccgg taagtatcgt ttgcatatcg gattcaatta
     2341 cttcctgctt ggtgatcgtt cctttgttgt caatactaat ggccctgggc ttattcaata
     2401 gtttggaaag tccttctgcc atgggtcctt cattatcctg tttatcactg tcaaaactca
     2461 tatcctgtcc catcatactg gacgttaaca gcactttcgt agcagtggcc tgcaggtcag
     2521 tgccttcagg attattgttc atcacttcaa tcaggctaat actttttgaa tcaattaaag
     2581 tttccatggt ttgtcccatc acttcagctg aactcgtcgt cttgttggtg gtttccacct
     2641 ggtacttctg tcctttttgt atatcaattt ttgtgggttg cgaaaaaccg gtagtgatgc
     2701 ccagcagcga aagagataaa gcggataagg ttatttttag cataacttat attttgattg
     2761 taaaactaat acggaataag taattcgatg caggtttact gacaagctgc agaaatttgg
     2821 gtgtcaatgg tttttttcag ttcccggtgt gcatcggctt tttcttctcc caatagattt
     2881 tttacaaaaa ttaaatacgc ataaccccgg caatagtcga tcatcggcca ggtgccaaat
     2941 aatcccgggc tggccagcgc agtggcaata ccattctttt cttcgatagc ccagcttccg
     3001 gtagtatagt tgaagcctga tgcagatttc ggtgcatatt tgatctgttc aggtttgttc
     3061 tgcactttca tcatctcatt aacggagttt tcactgagta tctgcacccc gttatatttt
     3121 cctttgttca atagcatcac caggaatttc atgtaatctt ccgctgttga cttggcacca
     3181 ccggaaggat tcaccgggcc accatcaata ttggtaaaac tggtcttccg catacccagc
     3241 gggtataata atttggtacg gataagcaca tcgaattttc ttttgctcac cacttccagt
     3301 acacgaccgg ctatattcaa accgatatta ccataccaga aatcggtacc ggtattggcc
     3361 ctgatctccc gggcagcaaa tgcattcact tcttcttcca gggtcgcaaa cttttttcgt
     3421 tgcgttaatt tcttcagcag accaccttca tcttcaatac ccgtcatatg gctcaggcaa
     3481 tgacggatgg taatatatcc tttaaaatat ttatcaaact ccggaatata tttaaccact
     3541 ttatcatcca gtgaaatttt accttcatcc acgaactgca tcacaagggc agcggtgagc
     3601 catttgctgc aactggcgat cggtgcctgg gttttcatcg taaagtcacc catttctttt
     3661 ttatagacca gcgaatcacc tttccaaatc atcgccactg cattagtccc gaataatttt
     3721 tgtttggcct gcaattcagt ttccagttcc gtccagttgt attgagcgga aacaggctgt
     3781 aagaatagca ggaatgccgc taccgacaag actttcaggc agatagaact gactttatat
     3841 gacatagtta cggggtttga ggggttggat gaatatttga gtatagattt tatacataat
     3901 taatatggaa gcactacgtt cactaaagct gtacacgaaa taaacaaaga ttttcagtgt
     3961 atgttgttaa aaactggtgt tccactgata aaaatataaa caaatgaatt taggaaactt
     4021 caccatcaaa gcagccgagg ctttccagca ggcccagcaa atagctttta atgcgcaaag
     4081 tccgaatatc gagacggagc atatcttaaa ggcgttgctc gaccagcagg attcgccggt
     4141 tgaatatttg ctgaaaaaaa ataacgttac cgttaacctg gtagagacca aactggatga
     4201 gctcatcagc aaattaccca aagcatcagg agaagcagcc cagcaaataa gccgggaagc
     4261 gaatagtgta gttttaaggg ccggtgcagt acttaaacaa ttcaatgatg aatttgtaac
     4321 cacagaacat ttattgcttg ccattgtgca ggggaatgat tccaccgcca agctgttgaa
     4381 gaatacagga ctaacagaga aaggattgat cgcagcgatc aaagacctga gaaaagggga
     4441 gacggttaca tcacaaacac aatcacagga attcaatgcg ctgaataaat atgccaagaa
     4501 tttaaatgaa ctggcaagac agggtaaact cgacccggtg atcggccgtg atgaagaaat
     4561 aaggagaaca ttacatatcc tttcacgcag aacaaagaac aacccgattc tggtaggtga
     4621 acccggtgtt ggtaaaacag ccatcgccga aggcattgct caccgaattg tgaatggtga
     4681 tgtgcctgaa aacctgaaat caaaaattat ctatgcactc gatatgggcc tgctcattgc
     4741 cggtgcaaaa tataaaggtg agtttgaaga acgtttgaaa ggagtggtaa aagaagtgag
     4801 cagcagtgac ggggagatca tcttattcat tgatgagata cacacactgg ttggtgcagg
     4861 tggcggtgaa ggcgccatgg atgcggccaa tattctgaaa cctgcactgg ccaagggtga
     4921 tctccgtgct gtgggtgcca ccaccctgaa tgaataccag aaattttttg aaaaagataa
     4981 agccttagaa agacgtttcc agaaagtgat ggtggatgaa cccagtgtgg aagatgctat
     5041 ctctatcctc cgtggattga aagaccgtta tgagacacac caccatgtaa gaattaaaga
     5101 cgaagccatc attgcagcgg tggaattatc cagccgttat gttacggatc gttttttacc
     5161 cgataaagcc attgacttaa tagatgagag tgcagccaag ctccgtttgg aaatgaactc
     5221 catgccggaa gaactggata cactggaaag gcagattcgt cagttggaga ttgaacggga
     5281 agctatcaaa agagagaatg atgaattgaa actgaaagaa ctgaataccg agattgccaa
     5341 cctggctgta tcaagagata cttacaaagc caaatggaac caggaaaaag aactggtaga
     5401 caatgtgcag aatgcgaaag aagaagctga acgcttaaaa cttagcgctg aacaggcaga
     5461 acgtaatggt gattatggcc tggtagcgga gatccgttat ggaaagataa aggagcagga
     5521 aaagctaatt gctgatctta ccagacaaat agaggaaagt ggtgaaaaac gtttgctgaa
     5581 agaagaagta gatgccgaag acattgctga aagtattgcg aaggcaactg gcataccggt
     5641 acaaaagatg ctgcaaagcg acagggaaaa attactgaag ctggaagagc atctgcatga
     5701 aagggtggtg ggacaggatg aagccatcac ggccgtggcc gatgcaatcc gcagaagccg
     5761 tgcaggtttg caggatccga aaaagccgat cggttccttc atcttccttg gtactaccgg
     5821 tgtgggtaag acagaactgg ccaaagccct ggcggaatat ttgtttgacg atgaaagcat
     5881 gatgacaagg atcgacatga gtgagtacca ggaaaaacat accgtgtcaa gattagtggg
     5941 tgcgcctccc ggttatgtgg gctatgatga aggcgggcag ctgactgaag ctgtgcggcg
     6001 caaaccttac agtgtggtat tgctcgatga gattgaaaaa gcacaccccg atgtttggaa
     6061 tgtactctta caggtattgg atgatggccg tcttaccgat aacaaaggcc gggtggttaa
     6121 ttttaagaat accattatca ttatgaccag caatatcggc agccacctga tacaagacgc
     6181 ttttgacggg gtgaatgaaa ataatatcga agaggccaat gctaaagcga aactggatgt
     6241 gatgaacctg cttcggcaga ccatacggcc cgagtttttg aaccgggtgg atgagatcat
     6301 catgttccag ccgctgctga aaaaagagat agccggcatt gtgaaaatac agctgaacaa
     6361 cctgcaaaaa ctggtagccg acagcggcat acaattagtg ttcagtgatt acgcactgga
     6421 attcctggca gaccagggct ttgatataca gtttggtgca aggcccctga agcggttgat
     6481 acaaaaggaa attgtgaacc agttatcgaa gcggatactg gcaggagata ttgataagac
     6541 aaaaccggtg ctggtggatg tgtttgatgg agtggtggtg tttaggaatg agacgccgga
     6601 gaaagtgaag taaaaaatca aaagtaaaaa gccctgacga gagttggggc tttttaagta
     6661 gtataaaacg gaataaaagg ggaattctca gtaatcttaa ttcctggaaa tttttgctta
     6721 atgtcccatc catcgagcat aatacttgtt atttttaaat gggttccatc tttaaagtaa
     6781 atcgttgtta gagaaaagtt attccagggg cacttgcttg cggtatttct atcaataata
     6841 atttcgtgta tatcgacttt gttgaacttt ttgagattgg cttcatttcc gaagaaaaac
     6901 atttttgctc cttgggataa ttggagtatc attccttttg aatggttgta ataatttatg
     6961 gtcaaccaca aattcactcc tccaattaat acaaatgaca tcagaaaaac tattacagtt
     7021 aaaacttgat tatc
//
LOCUS       DAORTI010000157         6945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_12477_length_6945_cov_6.723657, whole genome shotgun sequence.
ACCESSION   DAORTI010000157 DAORTI010000000
VERSION     DAORTI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12477_length_6945_cov_6.723657"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..608
                     /locus_tag="PLO70_14615"
                     /pseudo
     CDS             27..608
                     /locus_tag="PLO70_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            657..1274
                     /locus_tag="PLO70_14620"
     CDS             657..1274
                     /locus_tag="PLO70_14620"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQZ75751.1"
                     /translation="MSLSDFADETASESPAPGGGSIAAYTGALGVSLATMVANLSSHK
                     KGWDDRWEEFSNWADKGELYKKELIRLVDADTKAFNQIMTAFGLPKSTEEEKATRTKA
                     IQEATKFAIDIPFKVMQASYDSMEVIKAMAATGNPNSISDAGVGALCARSAVMGAFMN
                     VRINAAGYDDKDFVADILNRGREIENKTIALEAEILKIVDGKIGN"
     gene            1255..2355
                     /locus_tag="PLO70_14625"
     CDS             1255..2355
                     /locus_tag="PLO70_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQZ75752.1"
                     /translation="MEKSETKAPAAGPTLNEMQHLKIYNKQDVLSLTKLRRFETKLGE
                     RLQVIHDVAEMENSISGSSAKYVLFGIPEDLGAKGNLGIGGTDTLWIPFLQSFLNVQS
                     NDFLDGNEILLVGHFDFGDIQYLIDTTAKSDDEKIEAYRHAVNSIDDEVEHLVKIITE
                     AKKIPVVIGGGHNNSYPLIKGAAKGWHKAGVLPLAQINCINLDAHADYRPMEGRHSGN
                     AFRYAEEDGYLQKYCVIGLHENYLPQNIWIDIVNNPFIDCITFEDIFVHEKRTFLQSV
                     AHATGFTEDTLTGIDIDLDSIQNTLSSAMTPVGITPVQARQYVSFTAADSKPAYFYIC
                     EGATRLNDGRTDATSGKLISYLVSDFIKANSV"
     gene            complement(2356..4095)
                     /locus_tag="PLO70_14630"
     CDS             complement(2356..4095)
                     /locus_tag="PLO70_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75753.1"
                     /translation="MRSPLPLLVLLAFITALLAFRLQESKTESQSTSSNNYKKRNIAL
                     CSPDWTALKDRMEETDIPPIPGAGSYQWKISTENDSAQFYFNQGINMYYGFHIIEALA
                     SFKKASKLDPGSAMLYWAQALAYGPNINDLGYAASPDALLTSKKAVELSGKCSDKERM
                     LIQAQQIRYAADSTVSREKLNQLYVEHMKATYEKYPEDGDVAALYADALMLQHPWDLW
                     KIDGTPKPWTPQIREVLERVLKKNPNHPGANHYYIHVMEPSPFAAKALPSADRLGNLT
                     PGLSHMVHMPSHIYLRTGNYAKGVSVNETAVKSYKKIIPLYAPVTGNDFLYIIHNLHM
                     QTNNAMLAGRSGHSSSSASETKNSVPKEYLSIPGAMGNYIQYIYMTPVLVDIRFGNWD
                     KLLNIAKPEDSQVYATILYHLGRGMAFTHQSNLPAAKDELGQLQQLMKDSSLLIPFTP
                     FSPAIDGAIIAENILTGTIALKEKKYSEAIAAFEEAVETEENMVYNEPRDWMLNPKHY
                     LGNSLLKAGRIKEAKDILQKDLVTNNENGWALFGLWQSLTTEKKTAEATKMFARFKKA
                     FDKADIKLYGPVF"
     gene            4189..4710
                     /locus_tag="PLO70_14635"
     CDS             4189..4710
                     /locus_tag="PLO70_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75754.1"
                     /translation="MKRIFILFLVIISMEANSQNEFAATSFYNEFNKIYADAQEGFVK
                     NKGTKRISEYEELVDEHKAKWMLPLADSGKIVFPASGFPYVIYYFEPSKTRLKVDQRG
                     AYLRDAVATAFDKPLYTISETKIINNFPYTDTWFFTEQGEFRKTFAIFRQTIYNNNGK
                     FYLSFQIRGKKPL"
     gene            complement(4721..6688)
                     /locus_tag="PLO70_14640"
     CDS             complement(4721..6688)
                     /locus_tag="PLO70_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQZ75755.1"
                     /translation="MLAGLQNVTFEFGARTIVEDATWHIQPGERIGLIGYNGTGKSTL
                     LKLLVGEYLPSAGTIERSRTTSIGYLHQDLLSFDTNDSILQVALGAFEKVLQLEKEIE
                     ELGKELERTGDEKTLHEYSDMLYELETLDGYTIHHRTEEVLQGLGFSNADLQRPYKEF
                     SGGWRMRVLLAKMILQQPDLLLLDEPTNHLDLPSIEWLEKYLLHYQGSVVIVSHDKYF
                     LNRMVTKIVEVYQQKLHIYNGNYDYYEKEKTIRVEMQQKAYENQQDYIRQNERLVERF
                     RAKASKAAMAQSIMKKLDKLERIEDAEIERPNIRINFRVDKTPGKVLVELKNVSKSFG
                     DNVIIENSSVEIDRDDKIALIGANGKGKSTLLRIIAGVENFTNGERKWGHNVEESFYA
                     QHQLEALNVNNTILDEMKECGSQMNELELRGLLGCFLFSGDDADKKIKILSGGEKARV
                     ALAKTIVSKANFLMLDEPTNHLDMHSCDLLIDALNKYQGSIILVSHDRYFVSKTANKI
                     WEIVDHEIKEFKGGYEEWVAWKERMAKQDAENKKNGKVIEQPKPAEKKQNAPTPAPIL
                     KPQTGIVIDKEKKKELQRIQKQFQQLEEQIAKLTASKAALEASLADPGTYSDKSKFIQ
                     AEAAYKKAGEELEVLHKQYEKVFEKIMELEQ"
BASE COUNT         1961 a         1377 c         1440 g         2167 t
ORIGIN      
        1 cagcataatc agataagaag cgcagtaatc tgtcggtgat aagggcagga gagtatgaag
       61 gttttttcaa aaagattaaa caagcagaat ggaaacctga ttttggtcct gcagcgtttg
      121 atgccaaacg tggtggaaca gtaatcgggg ctagagattt cctggtggcc tataatgtga
      181 acctgaatac cacttctacc cgcagggcaa atgctattgc ctttgatgta agggaggctg
      241 gaagaaaatt gaaaacggag aatggagaat tcaaaacaat cccggggagt ttgaaatctg
      301 ttaaagcgat tggctggtat attgaagagt atggcgtggc acagatcagt atgaacctga
      361 cgaatattaa cattacgccg gtgcatattg catttgatga ggtttgtaag aaagcagaag
      421 caagaggaat aagggttact ggcagcgaat tggtgggtct agtaccatta catgccctat
      481 tggaggccgg taaatatttt ctgaaaaaac aaaaacgatc aaccggtgta tcagagaatg
      541 aactgataaa gattgcagta aaaagtatgg gtttggatga attggggccc tttaaaccgg
      601 aggaaaggat cattgaatat atgctccgca gtaaagccga cagtaaattg gtgaatatga
      661 gtttatctga ttttgcagat gaaacagcaa gcgaaagtcc tgcacctggc ggaggttcta
      721 ttgctgctta taccggtgcc ctgggtgtat cattagcaac aatggtggct aatttgtctt
      781 cacataaaaa aggctgggat gacagatggg aagagttcag taactgggct gataaagggg
      841 aactgtataa aaaggaattg atacggctgg tagatgcaga tacaaaagca tttaaccaga
      901 tcatgaccgc ttttggatta ccaaaatcaa ccgaggaaga aaaagcaact agaacaaaag
      961 caatacagga ggccacaaag tttgccattg atataccgtt taaagtgatg caggcgtcat
     1021 acgacagtat ggaagttata aaagcaatgg cagcaacagg taatccaaat tcaatttcag
     1081 atgccggagt gggtgccctt tgtgcccgta gtgcagtgat gggtgcattc atgaatgtgc
     1141 ggatcaatgc ggcaggatat gatgataaag attttgtggc tgatatactc aacaggggca
     1201 gggaaattga gaacaaaacc atagctttag aagcagaaat tttaaaaatt gtcgatggaa
     1261 aaatcggaaa ctaaggctcc tgctgccggg cctacattaa atgaaatgca gcacctaaaa
     1321 atatataata aacaggatgt tttatcgctg accaaacttc ggcgtttcga aaccaaactg
     1381 ggggaaagat tacaggtgat ccatgatgtt gctgaaatgg aaaattcgat ctccggctcc
     1441 agcgcaaaat atgttttgtt tggtatacca gaagatcttg gtgcaaaggg gaatttagga
     1501 attgggggca ctgatacatt atggataccc tttctgcaaa gtttcctgaa tgtacagagt
     1561 aacgattttc tggatggcaa tgagatatta ttagtcggcc attttgattt tggagatatt
     1621 caatacctga tagatactac agccaaaagt gatgatgaga aaatagaagc gtaccggcat
     1681 gcggtcaatt cgatcgacga tgaagtggaa cacctggtaa aaattattac ggaagcgaaa
     1741 aagattcccg tggtgattgg tggcggtcat aataattctt atcccttgat caaaggtgct
     1801 gctaagggct ggcataaagc aggtgtatta ccattggccc agataaactg tatcaattta
     1861 gatgcacatg ctgattacag gccgatggaa gggcggcata gcggaaatgc atttcgctat
     1921 gcagaagagg acggctacct ccaaaagtat tgtgtcattg gtttgcatga aaattatctg
     1981 ccgcagaaca tctggattga tattgtgaat aaccctttta ttgactgtat tactttcgaa
     2041 gatatttttg ttcatgaaaa aagaactttt ttacaatcag tggcacatgc taccggtttt
     2101 acagaagata cgttaacagg aattgatatt gatcttgatt ctattcagaa cactctcagc
     2161 agtgccatga cacctgttgg catcacacct gtacaggcaa gacagtatgt atcttttaca
     2221 gctgcagaca gtaaaccggc ctatttttat atctgcgaag gagccacccg gttaaatgat
     2281 ggcagaacag atgctaccag cggtaaactg attagctacc tggtaagtga ttttatcaag
     2341 gcaaattcag tttgatcaga atacaggtcc gtataatttt atatctgctt tatcaaaggc
     2401 ttttttgaac ctggcaaaca tttttgttgc ttcagcagtt ttcttttccg ttgtaagcga
     2461 ttgccataat ccgaataaag cccagccatt ctcattatta gtgacgaggt ctttctgcaa
     2521 tatgtctttt gcttctttta tacgaccagc tttcagcaat gagttgccga ggtaatgttt
     2581 cggattcagc atccagtccc ttggttcatt ataaaccata ttctcttctg tctctacggc
     2641 ttcttcaaaa gcagcaatag cctcgctgta tttcttttct ttcaacgcaa ttgttcctgt
     2701 taaaatattt tcagcaataa tagccccatc aatagcaggt gaaaagggag tgaatggaat
     2761 taaaagactg ctgtctttca taagttgctg taactgtccc agttcatctt ttgctgccgg
     2821 aagatttgac tgatgagtaa atgccatacc cctgcccaaa tgatacagaa tagttgcata
     2881 cacttgtgaa tcttccggct ttgctatatt cagaagttta tcccagttgc caaacctgat
     2941 atcaaccagc accggcgtca tataaatata ttggatataa ttacccattg ctcccggtat
     3001 tgacaggtat tcttttggga cactgttttt tgtttcgctg gctgaagagg aagaatgccc
     3061 cgaacgtccg gccagcattg cattattggt ttgcatatgc aggttatgaa tgatatacag
     3121 aaagtcattt cctgtaactg gtgcatatag cggaatgatt ttcttataac tttttacagc
     3181 tgtttcgttt actgaaacgc ctttggcata gttacctgtc cgtaaataaa tatgtgatgg
     3241 catatgtacc atatgtgaaa gacccggggt caggttacca agcctgtctg cactgggcaa
     3301 agctttggct gcaaaaggag agggctccat tacatggata taataatgat tagcgccggg
     3361 atgattcgga ttttttttca atactctttc taacacttca cggatttgag gtgtccatgg
     3421 tttaggcgta ccatcaattt tccataaatc ccatggatgt tgcagcatca gtgcgtctgc
     3481 gtacagggca gctacatctc cgtcttcagg atatttttca tatgtcgctt tcatatgctc
     3541 aacatataac tgattcagct tttctcttga aactgttgag tcagcagcat aacgaatttg
     3601 ttgagcctgt atcagcattc tttctttatc agaacatttt cctgaaagtt caacagcctt
     3661 tttactggtc agcaatgcat ccggagaagc agcataacca aggtcattaa tattaggccc
     3721 atacgccaat gcctgtgccc aatacaacat ggcagatcca ggatccaatt tcgacgcctt
     3781 tttaaaagag gctaaagctt caataatgtg gaagccataa tacatgttga taccctggtt
     3841 aaaataaaac tgggcgctgt cattttcggt gctgattttc cattggtaac tgccagcacc
     3901 tggaatggga ggaatatcgg tttcttccat cctatctttt aaagcagtcc agtcagggct
     3961 gcacaatgct atatttcttt tcttataatt gttgctgctg gtagattgac tttcggtttt
     4021 gctttcctgt aaacgaaagg ccagtagtgc tgtaatgaaa gccagcaata ccagtaatgg
     4081 cagaggcgac ctcataattg ttggtttaat ttataaaagt tacatctttt ctgtgaaatt
     4141 gtgtatcaca tcgttgagtt tatgcccctg ttggtataat tttgttatat gaaaaggata
     4201 tttatcctct ttcttgttat cattagtatg gaggcaaaca gtcagaatga atttgctgcc
     4261 acctcttttt ataatgagtt caataagata tatgctgatg cccaggaagg atttgtaaaa
     4321 aataaaggca ctaaaaggat aagtgagtat gaagaattag tagatgagca taaagccaaa
     4381 tggatgctcc cgctggcaga ttcaggcaaa atagttttcc ctgcttcagg gtttccctat
     4441 gttatttact attttgaacc atctaaaaca agactaaagg ttgatcagcg gggcgcttac
     4501 ttaagggatg ctgtagcaac agcgtttgat aagcctttat atactatatc tgaaactaaa
     4561 atcatcaata actttcctta tacagacacc tggttcttta ccgagcaggg tgaattccgt
     4621 aaaacctttg caattttcag gcaaacaatt tataataaca acggaaaatt ttatttgtct
     4681 tttcagatcc gtggtaaaaa accattgtaa aagaacataa ctattgttcc agctccatta
     4741 ttttttcaaa taccttttca tattgcttgt gtagtacttc cagttcttcg ccggcctttt
     4801 tataagcagc ttctgcctgg ataaattttg atttgtctga gtaagtaccc ggatcagcaa
     4861 gggaagcttc cagtgctgct ttgcttgcgg tcagctttgc tatctgttct tccaattgct
     4921 ggaattgctt ttgtatccgc tgtaattctt ttttcttttc tttgtctata acgattccag
     4981 tctgcggttt tagtattgga gcgggggtgg gggcattttg ctttttttca gcaggtttag
     5041 gctgctcaat taccttgcca ttttttttat tttctgcatc ctgctttgcc atccgttctt
     5101 tccaggccac ccactcttca tatccgcctt tgaattcttt tatttcatgg tcaacgatct
     5161 cccatatctt attagccgtt ttggaaacaa aatacctgtc gtggctaaca aggataatgc
     5221 tgccctggta tttattcaat gcatcaataa gcaggtcaca actatgcata tcaaggtggt
     5281 tggtaggttc gtccagcatc agaaagttgg ctttgctaac tattgtttta gccagggcaa
     5341 ctcttgcttt ttcgccacca cttaatattt ttattttctt atctgcatca tcaccactga
     5401 ataggaaaca gcccagtaaa ccacgaagtt ccagttcatt catctggctg ccgcattctt
     5461 tcatctcgtc aagaatagta ttattcacat tcaatgcctc taactggtgc tgcgcataaa
     5521 aactttcttc tacgttatgt ccccacttcc tttcaccatt cgtgaaattt tcgactccgg
     5581 ctatgattcg taacagggta gactttcctt taccgttagc cccgatcaac gcaattttat
     5641 cgtccctgtc aatttccaca gaactatttt ctataatcac attatcgcca aatgatttgg
     5701 aaacattttt taactctacc agcacttttc ccggcgtttt atctacccga aaattgatgc
     5761 gtatgtttgg tctttcaatt tcagcatctt cgatccgttc gagcttgtct aattttttca
     5821 tgatgctttg agccatggca gccttactgg cttttgccct gaagcgttcc accaaacgtt
     5881 cattctgtcg aatataatcc tgctggtttt cataagcctt ttgctgcatt tctacacgga
     5941 tggttttttc tttctcataa taatcataat tcccgttgta gatatgtaat ttttgctggt
     6001 atacttccac aatttttgtc accatccggt tcaggaaata tttatcgtga cttacaataa
     6061 caacagaacc ttggtaatgc aataaatatt tttccagcca ttcgatagag ggaaggtcaa
     6121 ggtggttggt tggttcatcg agtaaaagca gatctggctg ttgtaatatc atttttgcca
     6181 gtaataccct cattctccac ccaccactga attctttata gggtctttga agatcagcat
     6241 tggaaaaacc aagcccctgt aaaacttctt cagttctgtg atgaatagta taaccatcta
     6301 atgtttccag ctcatataac atatcactat actcatgcaa tgtcttctca tcaccggtac
     6361 gttccagctc tttccccagt tcttcaatct ctttttctaa ttgtaatacc ttttcaaaag
     6421 cgcctaacgc cacctggaga atggagtcat tggtatcaaa acttaaaaga tcctggtgca
     6481 ggtacccgat ggaagtagtc cggcttcttt caatcgtgcc tgctgagggc aggtattctc
     6541 ccaccaataa tttcagcagc gtagatttac cagtaccatt atatccgata agaccgatcc
     6601 tttctcccgg ctgtatatgc caggtggcgt cttctacaat tgttcttgca ccaaattcaa
     6661 atgtcacatt ttgcaatcct gccagcataa gtcaaattta tgaggctgca aaaataaagg
     6721 attacaactt ccggcagcct gcatttatct ttgtccaaat ttccggcaat atgaagaaga
     6781 tcatcgatat actgggtatt gttctaccgg cgctgataat catccttggt attacccgtg
     6841 tttttatgac taagacaaaa ggagtcaacg ggttgaccat gttatttgcc attctgctct
     6901 tattggtggg tataatccgt tattcagctt ttaggggagg agatg
//
LOCUS       DAORTI010000158         6796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_12910_length_6796_cov_6.170895, whole genome shotgun sequence.
ACCESSION   DAORTI010000158 DAORTI010000000
VERSION     DAORTI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12910_length_6796_cov_6.170895"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3614)
                     /locus_tag="PLO70_14645"
     CDS             complement(<1..3614)
                     /locus_tag="PLO70_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQZ75756.1"
                     /translation="MLNHIIRFSIRNKLIIGLLMFGLIGWGVYSFTKLPIDAVPDITN
                     NQVQVITVSPSLAAPEVERLITFPIEQATASVPGIIEIRSFSRFGLSLVTIVFNDNTD
                     VYWARQQVSERLQQVRSEIPADAGNPFMGPVTTGLGEIFQYAVRPKKGYESKYTATEL
                     RTIQDWIVRRQLLGTPGVADVSSFGGNLKQYEVAVNSDRLKSMNVSVDDVFNALQQNN
                     QNTGGAYIEKGPTVLYIRSEGLAKGIEDIGKMFVKHTTDGIPVYIRDVAEVRIGTAVR
                     YGAMCFNDEGEVAGAIVMMLKGGNSSDVISNVKERIKQIEKTLPEGVMIEPFLDRTKM
                     VNHAISTVEKNLIEGALIVIFILVIFLGNLRAGLIVASVIPLSMLFAVIMMNAFGVSG
                     NLMSLGALDFGLIVDGAVIIVEAVMHRLYHSRHYAVNNSITQAQMDAEVENSSGKMMN
                     SAVFGQIIILIVYLPILALVGIEGKMFKPMAQTVSFALIGAFILSLTYVPMITSLFLS
                     KKMKHKENFSDKMMNWIQRMYEPALKKALAFPRIITGVVIAVFVFAVFILSRLGGEFI
                     PKLEEGDFAVDTRLLTGSSLSQTIEATQKTAGILLKKFPEVEKVVTKIGSGEIPTDPM
                     PIEASDMMVILKEKKEWTSAKTFDELANKMSKALEDVPGVTAGFQYPVQMRFNELMTG
                     ARQDVVCKIFGEDLDTLAAYSKKLGDVVNTVKGARDLYVETVTGLPQIVISYNRDAMS
                     HYQLQIADVNRVIRTAFAGESAGLIYENERRFDLVVRLSRQSREDLSDVQNLLIATPM
                     GTQIPLYQVATVEIKEGPNQIQREDAKRRIVVGFNVRGKDVQSIVNELSKKVEAQLKL
                     PAGYYITYGGQFENLVEAKKRLSIALPLALLLIFIMLYFAFGKLKYGVLIFSAIPLSA
                     IGGVLALWILGMPFSISAGVGFIALFGVAVLNGIVLITEFNRLKNQGMHDLKFIIMEG
                     TKVRLRPVLMTASVASLGFLPMALSQGAGAEVQRPLATVVIGGLITATFLTLVLLPIL
                     YMWIEREKPKKTRRKVPVAPATIILLLLASSFVQAQDIEGKKMKLEEMLQLAGKQNLS
                     IQSLQKESAYWKQLQAAVFEPAKTQLGAEYGNINSLNNDTKFMLGQTFNLPVVYKRQK
                     EFYLSNEKAQTAMVGWKQLELNKEVKLVFWQMADLLARQKLLLRLDSVYSRVLQAAEL
                     R"
     gene            complement(3668..4051)
                     /locus_tag="PLO70_14650"
     CDS             complement(3668..4051)
                     /locus_tag="PLO70_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75757.1"
                     /translation="MKRVISILFLSAYLFSFTELHQLLKIPVLIEHFHEHRQEENSIS
                     FWSFLRMHYLEKIVVDKDYQRDQQLPMRDADCGMMTVSNLYECQHIAVEISLPSERTR
                     EFTFYDEFSKPRFAAYDIFQPPRIS"
     gene            complement(4096..6378)
                     /locus_tag="PLO70_14655"
     CDS             complement(4096..6378)
                     /locus_tag="PLO70_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75758.1"
                     /translation="MKQKLLLTFFTFLAVGVYAQQSLRGEVLNEQTREPVPGATISIS
                     DSSYSVSADDQGRFSISIPAGLSLRITATGYETKMIIPDNGNRLIIKLKSLQKELETV
                     VVTGTMKAVRRSESPVAVEVYTPQFLKKNPAPSIFESLQNVNGVRPQLNCSVCNTGDI
                     HINGLEGPYTMITIDGMPIVSSLASVYGLFGIPTQLIDRIEIVKGPASGLYGSEAIGG
                     LINIITKSPDKAPLFTANMMSTNWLEHSLDIGTKLKLSKKISSLLGINYFNYQQPLDK
                     NNDQFTDVTLQHRISVFNKFSFARKNNRIATLAGRYFYEDRWGGDMRWNRSFRGTDSI
                     YGESISTNRWELIGNYQLPIKEKVFFSFSATIHDQHSFYGTIPYNGKQKIGFTQLVWD
                     KQAGASHSLLTGLAGRYNYYDDNSTATIDTLTGKNVPDQYILPGLFFQDEWKLNSKQL
                     LLVGMRYDHHPLHKGIFTPRLAWKWSMKENQVFRLNAGTGFRVVSLFTEEHAALTGAR
                     EVEIRETLKPEKSYNMNLNYTWFRKWKNIYLNVDASAWYSHFTNQIIPDYDTDPNKII
                     YKNLDGFASSKGMAFNIEMNSNHRFKSLIGLTIQDVQKTEKNDQGQKIKQQPVLTEKW
                     SGTWVISYTFPAAAITIDYTGNIYGPMRLPLLSATDPRPGHSKIWSIQNIQFSTLLSR
                     KIELFTGLKNLLNWTPARNTPFIIARSHDPFDKKVEYDAAGRILATPENPYALTFDPS
                     YTYAPNQGIRFFAGLRFLVR"
     gene            complement(6544..>6796)
                     /locus_tag="PLO70_14660"
     CDS             complement(6544..>6796)
                     /locus_tag="PLO70_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75759.1"
                     /translation="NPAIYQRQANSDSYILMNAQVTKTVGKKNPVDLYIGGENLTNFL
                     QGDVIVSADSPFGPYFDASLVWGPVSGRLIYAGFRYKIK"
BASE COUNT         1986 a         1555 c         1259 g         1996 t
ORIGIN      
        1 cggagttctg cagcctgtaa cacacggctg tacacagaat ccaaccgcaa tagcaatttt
       61 tgccttgcta aaagatcagc catttgccag aacaccagct ttacttcttt gttcagttcc
      121 agctgcttcc agccgaccat cgctgtttgt gctttttcat tagacaggta aaattccttc
      181 tgccgtttat acacaacagg taaattaaac gtttgtccca acatgaactt tgtatcatta
      241 ttcaggctgt tgatattgcc atactcagca cccagctgtg ttttggcagg ctcaaaaaca
      301 gctgcctgta actgcttcca ataggctgat tctttttgta acgattgaat tgacaaattt
      361 tgcttccccg ccagttgcaa catctcttcc agtttcattt tctttccttc tatatcttgt
      421 gcctgcacaa aagaacttgc aagcagcaac aaaataatag tggcaggtgc tacaggcact
      481 tttcttcttg tctttttagg cttttctctt tcgatccaca tgtataagat cggcaacaag
      541 acgagtgtaa gaaatgtggc ggtgatcaaa ccaccaataa caacagtagc taatggtcgt
      601 tgcacttcag ctcctgcccc ttgacttaat gccataggca aaaatccgag cgaagcaaca
      661 gaggccgtca ttaatacagg acgcagtctg acttttgttc cttccataat aatgaattta
      721 agatcatgca taccctggtt cttcaaccgg ttgaattcag taataaggac gattccattt
      781 aaaaccgcca ctccaaacaa tgcaataaaa ccaacaccgg cagaaatact gaatggcatg
      841 cccagtatcc ataaggccag cacaccaccg atagcagaca aaggaatagc tgaaaagatc
      901 aatacaccat acttcagttt gccaaaagca aaatagagca taatgaaaat gagtaacaaa
      961 gcaagcggca aagcaatact caaccgcttt ttcgcctcta ccaggttttc aaattgtcca
     1021 ccataagtaa tataataacc agccggtaat ttaagttgcg cttctacttt tttactcaac
     1081 tcattcacga tactctgaac atctttaccc cttacattaa aaccaaccac aattctgcgt
     1141 ttggcatctt cccgctgtat ctggttgggc ccttctttga tctctacagt ggctacctgg
     1201 tataatggta tctgcgtacc catcggagta gcaatcagca ggttttgtac atctgaaagg
     1261 tcttctcgtg attgccggct caagcgtacg accaggtcaa atcttctttc attttcatag
     1321 atgagcccgg cgctttcacc agcaaaagcg gtacggatca cccggttcac atcggcaatt
     1381 tgcagttgat aatgtgacat agcatcacga ttgtagctga tcacaatctg ggggaggccg
     1441 gttaccgtct caacatacag atcccttgct ccctttaccg tatttactac atcgcccagc
     1501 ttcttggaat aggcagctaa agtatccaga tcttccccaa atattttaca aaccacatcc
     1561 tgtcttgccc ctgtcatcag ctcattgaaa cgcatctgta ccgggtattg aaatcctgct
     1621 gttactcccg gcacatcttc gagtgcttta ctcattttat tggccaactc atcaaatgtt
     1681 ttggcggagg tccattcttt cttttctttc aggattacca tcatatcaga agcttcgata
     1741 ggcatcgggt cagtaggtat ttctccggaa ccgatcttgg taaccacctt ttcaacttca
     1801 gggaactttt tcaataaaat acctgcggtt ttttgtgttg cttctatcgt ttgcgacaaa
     1861 gagctgccag tcagcaacct ggtatctact gcaaaatcac cttcttccag tttaggaata
     1921 aattcaccgc ccagtctcga aaggatgaat accgcaaata cgaatactgc gataacgaca
     1981 ccggtaatga tcctcggaaa cgctaaggcc tttttcaatg caggctcata catacgttgt
     2041 atccagttca tcatcttatc cgaaaaattc tctttgtgct tcatcttttt actgagaaac
     2101 agagaagtga tcatcggcac ataggttagt gataagataa atgcaccaat taaagcgaaa
     2161 gaaacagtct gtgccatcgg tttaaacatc tttccttcaa tacccactaa tgcaaggatc
     2221 ggcaggtata ctatcagaat gataatctgg ccgaaaactg ctgagttcat catctttccg
     2281 gatgaatttt ctacttcagc atccatctgt gcctgtgtga tactattatt gaccgcataa
     2341 tgccggctat gatacaagcg atgcatcacc gcttccacaa tgatgacagc accatccacg
     2401 atcagtccaa aatccagtgc tcctaaactc atcaggtttc cggaaacacc aaatgcattc
     2461 atcatgatca ctgcgaatag cattgacagc ggtataactg aagcaacaat cagtccggca
     2521 cgaagattac cgagaaaaat aaccaggata aaaataacga tcaaagcacc ttcgatcagg
     2581 ttcttttcca ccgtgctaat ggcatgattc accatttttg tccggtcaag gaacggttca
     2641 atcattacac cctcaggtaa tgttttttct atctgtttga ttcgctcttt cacattactg
     2701 atcacatcag aagaattacc ccctttcagc atcatgacaa tggcgccggc tacttcccct
     2761 tcatcattaa aacacatagc accataccta accgctgtac caatacgaac ttctgccaca
     2821 tcccgtatat aaacgggaat tccatcagtt gtgtgtttta caaacatctt tccgatatct
     2881 tcaatacctt ttgccaatcc ttcgcttctg atatataaaa ctgttggccc tttttctata
     2941 taagcaccac cggtgttctg attgttttgc tgaagcgcat taaaaacatc atctacagaa
     3001 acgttcatac ttttcaaacg gtcactatta accgctactt catactgttt caggttacca
     3061 ccaaaactac ttacatcggc cacaccgggt gttcccaata actggcggcg aacgatccaa
     3121 tcctggattg tccgtaattc tgtagccgtg tatttactct catacccttt tttgggtctt
     3181 acagcatact gaaatatctc tcccaaccct gttgttactg gtcccatgaa aggattacca
     3241 gcatcagcag gtatctcaga ccttacctgc tgcaaacgtt cagatacctg ctgcctggcc
     3301 caatacacat ctgtattatc attaaaaaca atagttacaa gagacaaccc aaaacgggaa
     3361 aaagaacgaa tttcaatgat cccgggtaca ctggcagtag cctgttcaat gggaaatgta
     3421 atgagacgtt caacttcagg tgcagctaag gaaggtgata ctgtgatcac ctgcacctgg
     3481 ttattcgtaa tatcaggaac agcatcaatg ggtagtttgg taaaagaata gactccccag
     3541 cctattaatc caaacattaa taacccaatg atcagcttgt tccgaatgga aaagcggata
     3601 atatgattaa gcataatttt ttttacttgt tcagtaaatc tggttgaaat agaataaaaa
     3661 tgaatgatca ggatatacgg ggaggctgga aaatatcgta agcggcaaac cggggtttac
     3721 tgaattcatc ataaaaagtg aattcccggg tcctttctga gggtaaacta atttcaacag
     3781 caatgtgctg acattcataa agattgctta cagtcatcat accacaatct gcatcccgca
     3841 ttggtaattg ctgatctctt tgatagtctt tatccactac aattttttcc aggtaatgca
     3901 tccttaaaaa agaccagaaa ctaatactgt tttcctcctg gcgatgttca tgaaagtgtt
     3961 ctataaggac aggtattttc agtaactggt gaagctcagt aaaggagaac agataggccg
     4021 aaagaaataa tatagaaatg actcttttca attttccaaa gataaaaaag aggggtgata
     4081 gttttgtgat tattatcacc ttactaaaaa gcgaagacct gcaaaaaaac ggatgccctg
     4141 gtttggtgca tatgtgtagg atggatcaaa ggtcagcgca taggggtttt caggagtagc
     4201 taaaattctg ccagcggcat catattccac ctttttatca aatggatcat gagaacgggc
     4261 aatgataaaa ggagtgtttc tggctggtgt ccagttaagc aggtttttta accctgtaaa
     4321 cagttcgatt ttccggctca aaagcgtgga gaactgaatg ttctgaatac tccatatttt
     4381 agaatgaccg ggtcgtggat ctgtggcaga caatagcggt aatcgcatcg gtccataaat
     4441 attacccgta taatcgatcg taatagctgc tgccggaaaa gtataactga taacccatgt
     4501 accactccac ttctctgtca atacaggttg ctgttttatt ttttgcccct ggtcattctt
     4561 ttcagttttc tgtacatcct gtattgtcag gccaattaaa cttttgaacc ggtgattgct
     4621 attcatttca atattgaaag ccattccttt ggatgatgca aatccatcca ggtttttgta
     4681 aatgatctta ttggggtcgg tatcataatc aggaatgatc tggttggtaa aatgagagta
     4741 ccaggcggaa gcatctacat ttaggtaaat attcttccat tttctaaacc aggtataatt
     4801 cagattcatg ttatagctct tttccggttt caatgtttct cttatttcaa cttcccttgc
     4861 tcctgtaagt gcagcatgct cttctgtaaa taagctgact acacgaaaac cagtacctgc
     4921 attcaggcgg aacacctggt tttctttcat tgaccatttc cacgccagtc tgggtgtgaa
     4981 tatcccttta tgcaatgggt gatgatcgta ccgcatacca accaatagca actgctttga
     5041 atttaatttc cattcatcct ggaaaaaaag cccgggaaga atatattgat ccgggacatt
     5101 ctttcctgtt aacgtatcaa tagttgctgt tgaattatca tcataataat tataacgacc
     5161 tgccagtcct gtcaataaag aatgggaagc accggcctgc ttatcccata ccagttgtgt
     5221 aaaaccaatt ttttgtttcc cattataagg tatggtacca taaaaagaat gctgatcatg
     5281 gatggtagcc gaaaatgaga aaaatacctt ttccttaatg ggtaactggt aattcccgat
     5341 cagttcccac cgattagtac tgatactttc cccataaata ctatctgtac cccggaatga
     5401 acggttccag cgcatatcac caccccagcg atcttcatag aaatatcgtc ctgccaatgt
     5461 tgcgatacgg ttattctttc ttgcaaaact gaacttattg aatacagaaa tgcggtgctg
     5521 taatgtcaca tccgtgaact gatcattatt tttatccagt ggctgctgat aattgaaata
     5581 attgataccc agtaatgaag aaattttttt actgagcttc agtttagtac caatatccaa
     5641 tgaatgctcc agccagtttg ttgacatcat attggcagta aacaaaggcg ctttatcggg
     5701 agatttagta attatattga ttaaccctcc aatggcttct gaaccataca aacctgatgc
     5761 cggacccttt acgatctcaa tccggtcaat caattgagtg gggataccaa ataatccata
     5821 cactgaggcc aggctgctga cgatgggcat accatcaata gtgatcatgg tataaggacc
     5881 ctccagtcca ttgatatgta tatctccggt attacaaaca ctgcaattga gttgcggacg
     5941 aacaccattt acattctgca gcgattcaaa aatactgggc gccggatttt tctttaaaaa
     6001 ttgtggcgta tagacttcta cggctaccgg gctttctgat cttctcacag ctttcattgt
     6061 gccggtcacc actaccgttt ccagttcttt ctgtaatgat ttcagtttta ttataagacg
     6121 attcccattg tccgggatga tcatttttgt ttcatagccg gtcgcagtaa ttcttaaaga
     6181 taatcctgca ggtatcgaaa ttgaaaacct gccctggtcg tcagcggaaa ctgaataaga
     6241 ggaatcacta atactgatcg ttgcaccggg aacgggttcc ctggtttgct catttaatac
     6301 ttccccacgc aatgattgct gggcatacac cccaacggcg agaaaggtaa aaaaagttag
     6361 aagtagtttt tgtttcatta ttcaacctat ctaaacatat ttttagacaa gactaaatat
     6421 atgtttaaac aaatataaac aatttaccaa tggcgaaaac tatcaatcag aatttaataa
     6481 ttcctgccgg cgaaaggtca gaactgcctc attctcaata atgaaaaaga tttgctattg
     6541 ttgtcatttg atcttatacc tgaaaccagc gtatatgagg cggccactaa cgggccccca
     6601 aaccagggaa gcgtcgaagt aagggccaaa cggactatca gcgctgacaa taacatcgcc
     6661 ctgtaaaaaa ttggtcaggt tctccccgcc aatataaaga tcaaccgggt tctttttacc
     6721 aacggtttta gttacctgcg cattcattaa tatgtacgaa tccgaattag cctgccgctg
     6781 gtaaatggcc ggatta
//
LOCUS       DAORTI010000159         6769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_12984_length_6769_cov_6.183348, whole genome shotgun sequence.
ACCESSION   DAORTI010000159 DAORTI010000000
VERSION     DAORTI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12984_length_6769_cov_6.183348"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLO70_14665"
     CDS             complement(<1..427)
                     /locus_tag="PLO70_14665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQZ75760.1"
                     /translation="MSGIHFYSYFTFMIGIIVQLAVSWLVVWLFEKGNLNCLGLLPVK
                     KRLTDFGLFFSITAACSVLSFLLQMLIARQSWQLNPDINLLLIMKGTWFVIKSVLFEE
                     LIFRGVLLYILIKKIGAVKAIFISAAAFGMYHWFSHELWG"
     gene            complement(440..2881)
                     /gene="priA"
                     /locus_tag="PLO70_14670"
     CDS             complement(440..2881)
                     /gene="priA"
                     /locus_tag="PLO70_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQZ75761.1"
                     /translation="MFAEVIIPAALPKNYTWSVPTHLLETVKVGCRVEVNLGKSKKYA
                     GIIKRLHEEKPEFFETKDIINVLDTAPVVFEQQLKLWEWIASYYMCSEGEVMAAALPA
                     HFKLSSETILVYNEEYGDDFSSLDHDEYIVGEALLLKKELNLNEVQQLLDSSHVYPVI
                     NRLINKKVCFVWEALKQTYAPKKETYVLLNPAYDNEDKLSDLLNNWTKAPKQMELLLS
                     YLHLLKTEGEVVKSSLLKKSGASDAQLKGLVEKNILRTEIKNIDRIQYLPKNVTIDFE
                     LSEGQHKAFEEVKEQLQSKAVCLLHGVTSSGKTNIYIKLIEEYIKQGKQVLYMLPEIA
                     LTSQVIRRLQKHFGGFIGIYHSKFSQNERVEIWNKVKNGELRVILGARSSVFLPYQNL
                     GLVICDEEHDTSFKQQEPAPRYNGRDAAIYFASLFEAKTLLGSGTPSLESYYNAATGK
                     YGLVELLQRYGDLKLPPIEMIDTRKIIQKDKSKVMLSPQLVEAVNEVLARSKQAILFQ
                     NRRGYAPYQVCNVCGWIPQCKYCDVSLTFHKLTNKLVCHYCGTTYPPVHTCAACGSDK
                     FAQRNFGTEKIEEQLQETFPDAKVARMDMDTVRGKNAHDVLIQQFEQKRIDILVGTQM
                     VVKGLDFDNVDLVGILDADGLLHFADFRVNERAFQLMEQVSGRAGRKEETGKVLVQTS
                     QPQHPLLQIVQQHDYKMMFAEELKKRKEFAYPPFSRIIHLTFKHKIKEVVERAAHYYT
                     DALKNRYGQYIVGPAEPVIGRIRNQFLMELLLKLPRDGKTINQCKKDLLEQIAILHQE
                     RSFRSVTIVADVDAV"
     gene            complement(2923..3684)
                     /locus_tag="PLO70_14675"
     CDS             complement(2923..3684)
                     /locus_tag="PLO70_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQZ75762.1"
                     /translation="MNIVMKPLQWIYSIYAFITFIAIMLLILPFAFIASLFGRIKGGN
                     MMIRLCMLWADIWFPLIFIWHKKIYESPHDKKKAYIFVSNHISYLDAAIIVKAYRQPI
                     RPLGKIEMSKVPLFGFIYRNAIVTVDRSSAEHRAESVRILKSIINKGISVVVFPEGTF
                     NMGTTPLKSFYDGAFRVAIETQTPIKPVLFLDAYRRMHYRSLFSLAPGYSRILYLDEI
                     PVTGYTINEVGRLKDEVYAIMEKKLIEYGAAWRKE"
     gene            complement(3684..4157)
                     /locus_tag="PLO70_14680"
     CDS             complement(3684..4157)
                     /locus_tag="PLO70_14680"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HQZ75763.1"
                     /translation="MNKKWVPYLIIALLAITLFVINQYKSSKEPVPKPKVTDNDRPKD
                     PASNPTDRDKGFDRRTSYLKYSNHAKCRMQCRKITQAEVEEIMREGKINYNKSDLDNA
                     RCPRYAVEGVTSDDQRVRIVYAQCNESTTVVTVIDLETDFKCDCPGDDDKYKNRN"
     gene            4248..5534
                     /gene="mtaB"
                     /locus_tag="PLO70_14685"
     CDS             4248..5534
                     /gene="mtaB"
                     /locus_tag="PLO70_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQZ75764.1"
                     /translation="MAESRTVAFHTLGCKLNFSETSSLSRMLELEGFEKKEFDDLADI
                     YVINTCSVTDNADKECRQLVRRIQRKSPESLVVITGCYAQLKPKEISEIPGVDLVLGA
                     AEKFNIAQHIRELAKGDSAKICSCDIEEVSGFHSSWSVNDRTRTFLKVQDGCDYNCSF
                     CTIPMARGKSRSDSIANVVHNAEQLAANGVKEIVLTGVNLGDFRPPTSAGGDLAASTK
                     ENFYSLIQELEKVSGIHRYRISSIEPNLLTNEIIEFVANSSTFMPHFHIPLQSGSNKI
                     LGLMRRRYKRELYDERVALIKTLMPHCAIGVDVIVGFPGETEEEFNETFEFIHSLDIS
                     YLHVFTYSERDNTHALSMKPVVPMQIRNERNKTLRNLSYMKMQYFTQQHSGQTRKVLF
                     EGHDKTGMMEGYTDNYIRVTTPFRQEWVNEIVEWKI"
     gene            5560..5634
                     /locus_tag="PLO70_14690"
     tRNA            5560..5634
                     /locus_tag="PLO70_14690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5595..5597,aa:Val,seq:gac)
     gene            5711..6001
                     /locus_tag="PLO70_14695"
     CDS             5711..6001
                     /locus_tag="PLO70_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75765.1"
                     /translation="MQKYQVTIQFEMNDEFMDLVPPHRTYINFLINKGTIDHYAVSME
                     TQRSWITLNAEDKKEVEIILQKSPLFKFWTFEIDELFVLDGQHYRLPEVNPN"
     gene            complement(6046..6594)
                     /locus_tag="PLO70_14700"
     CDS             complement(6046..6594)
                     /locus_tag="PLO70_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQZ75766.1"
                     /translation="MATTYCEFVQNAGKETVHRYYHDHEYGFPIEDDNLLFARLVLEI
                     NQAGLSWETILKKKDNFFRAFDDFNIDKVARYTDKKKEKLMQDAGIIRNRLKIEATIH
                     NANAIKSIQKEAGSFKSWLDAHHPKTKEEWVKIFKQTFRFTGGEIVNEFLMSTGYLPG
                     AHTAGCPVYKKVMKHKPKWNQK"
BASE COUNT         1916 a         1513 c         1297 g         2043 t
ORIGIN      
        1 tcccccaaag ctcatgagag aaccagtggt acataccaaa tgctgcagct gaaatgaaaa
       61 tagcttttac agcaccaatt tttttaataa ggatatagag caacactccc cggaagatca
      121 gttcttcaaa cagcactgat ttaataacaa accaggttcc tttcatgatc aacaataaat
      181 ttatatcagg gtttaactgc cagctttgcc tggcaatcaa catttgcaat aaaaaactga
      241 gaacagagca agctgctgtg atactaaaga acaaaccaaa gtcagtcagc ctttttttaa
      301 caggcagtaa tccaaggcaa ttcaaattac ctttttcaaa caaccatact accagccagg
      361 aaacagcgag ttgaacgata atgccgatca tgaaagtaaa gtacgaataa aagtgaatcc
      421 cactcactac acactttttt tacaccgcat ccacatctgc tacaatcgtc acactccgaa
      481 agcttctttc ctggtgcagg atggcaatct gttccagcag atcctttttg cactggttaa
      541 ttgttttacc atcacggggc aatttcaaca atagttccat caggaactgg tttcttatac
      601 ggccgataac cggttctgcc ggaccaacga tgtactgacc gtacctgttc tttaatgcgt
      661 cggtgtagta atgtgccgct ctttccacca cttctttgat cttatgttta aaagtgagat
      721 gaatgatcct cgaaaaaggc gggtaagcaa actcctttcg tttcttcagt tcttccgcaa
      781 acatcatttt ataatcatgc tgctgtacaa tctgcaacaa cggatgctgc ggctgcgagg
      841 tttgcaccaa cacttttccg gtttcttctt tccgcccggc acggccactc acctgctcca
      901 tcaactggaa ggcccgttca tttacccgga aatctgcaaa atgcaataag ccatctgcat
      961 ctaatatgcc aacaaggtcc acattatcaa aatcaagtcc cttcaccacc atctgggtac
     1021 ctacgagtat atcaatccgt ttttgttcaa attgctgtat gagcacatca tgggcatttt
     1081 ttccccgcac cgtatccata tccattctcg ccactttcgc atcggggaaa gtttcctgta
     1141 attgttcttc tattttctct gtaccaaaat ttcgttgagc aaatttatcg ctgccacagg
     1201 ctgcacaggt atgaacggga ggataggtgg taccgcaata atgacaaacc agtttgtttg
     1261 tgagtttatg aaaggtcagc gacacatcgc aatatttaca ttgcggtatc cagccgcaga
     1321 cattgcatac ctggtaaggt gcatagcccc ggcggttctg gaataaaatc gcttgtttac
     1381 ttcttgccag cacttcattc actgcttcca ccagttgtgg agagagcatc actttcgatt
     1441 tatccttttg aatgatcttc cgggtatcaa tcatttcgat tggcggcagt ttcaaatcgc
     1501 catatcgttg cagcaattcc accaaaccat atttgccggt ggcagcattg taataactct
     1561 ccagcgaggg agtgccgctt cccaataaag tttttgcttc aaacagtgat gcaaaataaa
     1621 tagctgcatc tctcccatta tatcttggtg ccggttcctg ttgtttgaaa gaagtatcat
     1681 gttcttcatc acagatcacc aaccccaggt tttggtaagg caggaataca gaggaccttg
     1741 ctcccaatat cactctaagc tcaccattct ttactttatt ccaaatctcc actctttcat
     1801 tctgcgagaa tttggaatga taaattccaa taaaaccacc gaagtgtttt tggagacgcc
     1861 tgatcacctg ggaggtcagt gcaatctccg gcagcatata cagtacctgt ttaccctgct
     1921 taatgtattc ttcaatcagt ttaatataaa tattcgtctt cccacttgag gtaacgccat
     1981 gcagcaggca tactgctttg ctttgcaatt gttcttttac ctcttcaaaa gccttatgct
     2041 ggccttcact gagttcaaaa tcgatggtta cattcttagg caggtattgt atacggtcaa
     2101 tattttttat ctcggtacgc aggatatttt tttcaaccaa tcccttcaac tgtgcatccg
     2161 aagcacctga tttttttaac aaggaggatt tcaccacttc accttccgtt tttaataaat
     2221 gcaggtaact gagcaataac tccatttgtt tgggagcttt cgtccagttg ttcagcaagt
     2281 cagacaactt gtcttcatta tcataagcag gattcagtaa tacatacgtc tcttttttgg
     2341 gggcataggt ttgtttcaat gcttcccaaa caaaacaaac ttttttattg atcagccggt
     2401 tgatgacagg ataaacatga gaagaatcca gtaattgctg gacctcattc aggttaagtt
     2461 ctttttttaa caacaaagcc tccccgacaa tatactcatc atggtccagt gaagaaaaat
     2521 catcgccata ttcttcatta taaactaata tcgtttcact ggagagtttg aaatgcgccg
     2581 gcagcgccgc cgccattact tccccttcac tgcacatgta gtatgaggct atccattccc
     2641 acagttttaa ttgctgttca aataccaccg gcgctgtgtc cagtacatta ataatatctt
     2701 tagtctcgaa gaactctggt ttctcttcgt gcagtctttt tataatgccg gcatattttt
     2761 tactttttcc aaggttcacc tctaccctgc agcctacttt tactgtttcc agcaaatggg
     2821 tgggtacaga ccatgtataa tttttgggta atgccgcggg tatgataact tctgcaaaca
     2881 aataagtgaa tttggaggct caatttagct gaaaaaagaa tactactcct ttcgccaggc
     2941 tgcgccatat tcaatcaact ttttttccat gatggcatac acttcatcct tcaatcttcc
     3001 cacttcattt attgtgtacc ctgtaaccgg aatttcatcc aggtaaagaa tgcggctgta
     3061 accaggagcc agggagaata aacttctgta atgcatccgg cgataggcat ccaaaaaaag
     3121 aacaggttta atgggagttt gtgtttcgat cgccacccgg aatgcgccat cgtaaaaact
     3181 tttaagcggt gttgttccca tattaaaagt tccttcggga aaaaccacca cagaaatgcc
     3241 tttattaatt atagacttca gtatcctgac actttcggcc ctgtgttcag cactgcttct
     3301 atccactgtt actatagcgt tacggtaaat aaatccaaac agcggaacct tgctcatttc
     3361 tattttaccc agcggccgta taggttgccg gtatgcttta acaatgatcg cagcatcgag
     3421 gtaagaaata tgattactga caaaaatgta agccttcttt ttatcatgcg gactttcata
     3481 tattttctta tgccagataa aaatcaatgg gaaccaaata tctgcccaga gcatacatag
     3541 tcttatcatc atgttgccac ctttaatgcg tccgaacaga ctggcaataa aagcaaaagg
     3601 aagtatcagc agcatgatcg ctataaaagt gatgaaagca taaatgctgt agatccattg
     3661 caatggtttc attactatgt tcatcagttc cggtttttat atttatcgtc atcaccgggg
     3721 cagtcgcatt taaaatcagt ttccaggtca attaccgtta ccactgttgt tgactcattg
     3781 cattgtgcat atacgatcct cacccgttga tcatcactgg tcaccccttc aacagcataa
     3841 cggggacatc ttgcattgtc gaggtcactt ttgttatagt tgatctttcc ttctctcatg
     3901 atctcctcca cttccgcctg tgtaattttg cggcattgca tcctgcattt tgcatgatta
     3961 ctgtatttca ggtaagaagt acggcgatca aaacccttgt ccctgtcagt gggattgctg
     4021 gcggggtctt tcggccggtc attgtcagta acttttggtt taggaacagg ttccttactg
     4081 cttttgtatt gattaataac aaaaagagtg atagccaaaa gggcaataat gagatatggg
     4141 acccatttct tgttcacttt caaacatttg gctaaaaata cctgaattta aaaaatcagc
     4201 gaaatcaaac ttaatcctgt cattcgtggt ttatctttgc cggctctatg gcagaatcaa
     4261 gaacagtggc atttcataca ctcggctgta agctcaattt ctccgaaaca tcttcccttt
     4321 cccgaatgct ggaactggaa gggtttgaga aaaaggagtt tgatgacttg gcagatatat
     4381 atgtgattaa tacctgttca gtaacagata atgcagataa agaatgccgt cagctcgttc
     4441 gccgtattca gcgaaaatca ccggaaagtc ttgttgtgat aactggttgc tacgcacagc
     4501 ttaaacctaa agaaatttcc gaaatacccg gagttgacct ggtgctgggc gctgcggaaa
     4561 aattcaatat tgcccaacat atccgtgaat tggcaaaagg tgattcagca aagatctgca
     4621 gttgtgatat tgaagaagtg tccggttttc attcttcctg gtcggtgaac gacaggacaa
     4681 gaactttttt gaaagtacag gacggttgtg attataactg ttctttctgt accatcccga
     4741 tggcaagagg taagagcagg agcgacagta ttgcaaatgt ggttcacaat gctgaacagt
     4801 tggcggctaa cggagtgaaa gaaattgtat taaccggagt gaaccttggt gatttccgcc
     4861 ccccaacctc cgcgggagga gatttggcag cttccacaaa agaaaacttt tacagtctca
     4921 ttcaggagct tgaaaaagtt tcaggtattc accgatacag gatttcgtct attgaaccta
     4981 accttttgac aaatgagatc attgagtttg tagccaacag ttctacgttc atgccgcatt
     5041 ttcatatacc gctgcaaagt ggcagcaaca aaatacttgg tttgatgcgt cgccggtata
     5101 aaagagaact gtatgacgaa agagtggccc tcatcaagac actgatgccg cattgtgcta
     5161 ttggggtgga tgtaattgtt ggttttcccg gagagacaga agaagaattc aatgaaacat
     5221 ttgaattcat tcattcattg gacatttctt acctgcatgt gttcacgtac agtgaaaggg
     5281 acaataccca tgctttaagc atgaaaccgg ttgtaccaat gcagatccgt aatgaaagaa
     5341 acaaaacgtt gcgcaatctg tcctatatga aaatgcagta ttttacccaa caacattccg
     5401 gacaaacccg taaagtattg tttgaagggc atgataaaac aggtatgatg gaaggatata
     5461 cagacaatta tatccgcgtt actactccat tcaggcagga atgggtgaat gaaatagtgg
     5521 aatggaaaat ataagcaaag ctcttatttt tacacctatg gaacgttagc tcagttggtt
     5581 cagagcacta ctttgacaga gtaggggtca ctggttcgag cccagtacgt tccactctcc
     5641 tttttacacc taataacttt ctttttctgc ccggtgcaac tttcctacat ttaagttgtc
     5701 atttatacag atgcagaaat accaggtgac catacagttt gaaatgaacg atgagtttat
     5761 ggatttggtt cctcctcacc gcacttatat taacttcctg atcaataaag gaaccattga
     5821 ccattatgct gttagcatgg aaacccaacg atcatggatc acactgaatg cagaagacaa
     5881 gaaagaagta gagattatcc tgcaaaagtc tcccctattt aaattctgga ccttcgagat
     5941 tgacgaattg tttgtattgg acggacagca ttatcgctta cccgaggtaa atcccaacta
     6001 aacaggaata ccctgctcaa ttcatttctc tttatcaatc acttttcact tttgattcca
     6061 cttcggttta tgtttcatca cttttttata taccgggcag ccggcagtat gtgcaccggg
     6121 taaataaccg gtactcatta aaaattcatt gacaatttca ccaccggtaa aacgaaaagt
     6181 ctgcttaaaa atctttaccc actcttcttt tgttttgggg tgatgcgcat ctaaccagct
     6241 tttaaaagag cctgcttctt tttggatcga tttaatagca ttcgcattat gaatagttgc
     6301 ctctatcttc aacctgtttc tgataatacc ggcatcctgc atcagtttct cttttttctt
     6361 atccgtatat cgggctactt tatcaatatt aaaatcatca aatgcccgga agaaattatc
     6421 tttctttttc aggatcgttt cccagctcaa tccggcctgg ttgatttcta ataccagccg
     6481 ggcaaataac aggttgtcgt cttctatcgg aaaaccatat tcatgatcat gataataccg
     6541 gtgcacggtt tctttccctg cattttgcac aaattcgcag taagtggttg ccatacagtc
     6601 aattttgcct gaaaatagat aaaaaatatt ttgtctgaaa agtcctgcac ccgtatcttt
     6661 gccgtcccga aaaagggagt ttagctcagc tggttcagag catcccgact taaagtcggg
     6721 agaatcggcg gttcgaaaat aaaattgaaa taatcgggag tttagctca
//
LOCUS       DAORTI010000160         6759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_13014_length_6759_cov_5.783860, whole genome shotgun sequence.
ACCESSION   DAORTI010000160 DAORTI010000000
VERSION     DAORTI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13014_length_6759_cov_5.783860"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLO70_14705"
     CDS             <1..253
                     /locus_tag="PLO70_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ75767.1"
                     /translation="DESVSALDVSVQAQVLNLINDLKKEFGFTVIFISHDLSVVRYIS
                     DRIMVMNKGKIEETGLADEIYFNPQSAYTQKLIESIPKG"
     gene            complement(274..4116)
                     /locus_tag="PLO70_14710"
     CDS             complement(274..4116)
                     /locus_tag="PLO70_14710"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQZ75768.1"
                     /translation="MKRFLIFFVATFSFVLVQAQANIKEKKYPSLLWEITGNGGKKPS
                     YLIGTMHVSSKLAFNLPDSFYIAIRKAQVVALETNPETWQEDMSKYDLGEEGYYGASA
                     GYGEYTSMPEDYLGINTLKFYKYDAKIERALFSNPSTINNLLYRSYGNESSDFEEDTY
                     LDMYIYQCGKKWGKKVTGVEDYGQSMRLMAEAYKDAAKDKNKKDRSYGDVDEEYAADR
                     LQEAYRKGDLDLLDSINTFNSFSAAFDEKFLYKRNEIQANSIDSILKSGSVLFVGVGA
                     AHLPGQRGVIEMLRAKGYKLRPIKMGERASREKDMVDKIRVPVSFHTETAEDGFFKVD
                     IPGKFYKFGEDAALDQKQYADMANGSYYMVTRIMTNAWMWNHTVDDVLKKVDSLLYEN
                     VPGKIIAKIPVTKNGYKGFDITNRTRRGDLQRYNIFVTPFEVLFFKISGNGDYVKLGD
                     EAKRFFGSIQLKEYKNDVEASAGWKKYSPGYGGFTVDFPYVPYTGNDGSWIFDAEDKT
                     TATHYRVIRSDIHNYNFAEEDTFDLGLMEESFMASEFIDSQLSRVQTTYKGYPALEAK
                     YRDKQGSIYAVRFIIQGPHYYTLIAHGRQELPEMNNFLNSFEIKPFIYQAAKKETDTT
                     LYFSVITPVFPEEKKIKLDIPRYQWMGGEGDYEDPESEDLLLESGAFRNKIVANDTTG
                     EKIYISFYRSPRYYYSKDSSLVHRDKFVSYRGDSSWIVKLFKTTELPGKMKVWEAILT
                     DSGSSRTIWTKTYYKNGINFSLTTQADTLTPPSDFVKIFYETFTPVDTLKGTNLFVKK
                     SGLFFEDLMSKDSVLQKRAIKHIGDIYLDSSDLPLLKKAISWFNWKEKKYLDTKKSLI
                     NKLGNIKTAAAANYLKELYYALDDTIELQYPVLENLLQHKTLHAYQVFRDIISTEPPV
                     LDFSTTDFYPYTPPTISAMGDVDYSYDDGKFLDELSDSLALTKTILPDLLPLLNLDDY
                     KSAVMKILGEMADSNIVKPADYEMYFSKFLLEARQELKKQSIAEKKKAIKMAEESKEE
                     RKPSYYYDEESEKDKGNEDLSLYAALLIPSWETNPVVRPLIGQMLASNDKQLKYNTLM
                     QMLKHNLSFPDTLLKYFGSLDDYRYTLYNDLKRMKKLDKFPPLYNNHTDLGKSALLAE
                     KSYGKPDSLVYIDRLKTEYKGKKGFVYFFKYKAKKDDLTWKLATVGLVSENTHEFLFE
                     DTIKVRLPKYSYEVFNYTGYNRYNFTGFTDTKADTNGMLAVQLKKELKKMLYSRRKSA
                     KEFYEGEKNGTMLSQHID"
     gene            4442..5491
                     /gene="queA"
                     /locus_tag="PLO70_14715"
     CDS             4442..5491
                     /gene="queA"
                     /locus_tag="PLO70_14715"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQZ75769.1"
                     /translation="MKLSQFRFDLPLNLIAQNPTKRREDARMMVVHRHTGEIENKHFR
                     DIIDYFDDKDCFVVNNTKVFPARMYGRKEKTGAKIEVFLLRELNKPNRLWDVIVDPAR
                     KIRVGNKLYFGEQEDLVAEVIDNTTSRGRTIRFLWDGTEEEFRAVLERLGETPLPKYI
                     KRKPDEEDKERYQTVYAKHEGAVAAPTAGLHFSRELIKRLEIKGIRFAEVTLHTGLGT
                     FRPIEVEDLSKHKMDAEYYNIDETACKVVNRAKEYGHRVCSVGTTTMRALESSFTAQK
                     LLKPSEGWTNMFIHPPYDFSIADSLVTNFHLPKTSLLIMTCAFAGYELAMEAYKKAIK
                     DKYRFFSYGDALLII"
     gene            complement(5559..6752)
                     /locus_tag="PLO70_14720"
     CDS             complement(5559..6752)
                     /locus_tag="PLO70_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HQZ75770.1"
                     /translation="MFLTFFASFAQSVPKDSVGYKSRKLTFEEANLVSSYYAQDGNNA
                     AVTGGIGTQKLTDISNTIDVKFTKWDKKDRKHSFDLEVGIDHYTSASSDKIDPFTVSS
                     ASHADTRIFPSLAWSMENEKKGSTLGAGISASSEFDYMSFGANISFAQKTKNRSGEFS
                     AKLQAYIDNLAYITPIELRPGGGGGGGNGEEESRKDYPSKARNSFSGSFSYSQVINQQ
                     LQIMFIADLIYQQGYLGLPFHRVYFNGAILPVIENLPDTRLKIPIGFRANYFLSDKVI
                     LRSFYRYYHDDWGLSAHTVDLETPIKINPFLSVTPFYRYYTQSAIDFFGGYKVHTAAD
                     QYYTSNYDLSKFSSNFYGAGFRIVPPKGVFGIQRLNMLELRYGHYTRTNGLNSDIISM
                     NLKFK"
BASE COUNT         1975 a         1455 c         1257 g         2072 t
ORIGIN      
        1 tgatgaatca gtttctgcac tggacgtaag tgtgcaggcc caggttctga atttaataaa
       61 tgatctgaaa aaagagtttg gcttcactgt catattcata tcgcatgact tgtctgttgt
      121 ccgttacatc agcgaccgga taatggttat gaacaaaggg aagattgagg aaaccggcct
      181 tgccgatgag atttacttca atccccaatc tgcttatacc caaaagctga tcgaatcaat
      241 acctaaaggc tgatagcctg aaaaagttac ggcttaatca atatgctgtg aaagcatcgt
      301 tccatttttt tcaccttcat aaaactcttt ggcacttttt cttcttgaat aaagcatttt
      361 tttaagttcc tttttaagct gaaccgctaa catcccattt gtatctgctt tagtatcggt
      421 gaaaccggta aaattgtacc tgttataccc ggtataattg aatacttcat aagaatattt
      481 gggaagcctg acttttatcg tatcctcaaa cagaaactca tgcgtatttt cagaaaccaa
      541 tcctactgtt gccagcttcc aggtcagatc atcctttttc gctttgtatt taaaaaaata
      601 aacaaagcct ttctttccct tgtattcagt tttcagcctg tctatgtaca ccagcgaatc
      661 aggtttgcca taggattttt cagccagtaa agcactttta ccaaggtctg tatgattatt
      721 atacaatggc ggaaatttat ccagcttctt cattcgcttc aaatcattgt acagcgtata
      781 ccggtaatca tcaaggctgc caaaatattt taaaagtgta tccggaaaag aaagattatg
      841 cttcagcatt tgcatcagtg tattatactt caattgttta tcattagacg ccagcatttg
      901 cccgatcaat ggccttacaa cgggattagt ttcccaggat ggtatcaata aagcagcata
      961 aaggctcagg tcttcatttc ccttgtcttt ctcactttcc tcatcataat aataggatgg
     1021 ctttctctct tccttacttt cctcggccat ctttattgct tttttctttt cagcaatgga
     1081 ttgcttctta agttcctgtc tcgcctccag taagaattta ctgaaataca tttcataatc
     1141 tgcaggcttt acaatattac tgtctgccat ttcaccaagt attttcataa ctgcactttt
     1201 atagtcatca aggtttaaca ggggaagcaa atccggcaga atagtttttg tcagcgctaa
     1261 agaatcagat aattcatcca ggaacttacc gtcgtcataa ctataatcga catcacccat
     1321 cgcagagata gttggcggtg tatagggata aaaatcagtc gtactgaaat ccaatacagg
     1381 tggctccgta ctgataatat cccgaaatac ctggtaggca tgcagggttt tatgctgcag
     1441 caggttttca agaacaggat attgaagctc tatggtatca tccagggcat aatacagctc
     1501 tttcaggtaa ttagcagctg cggctgtctt aatattaccc agtttattga tcagcgattt
     1561 tttagtgtca aggtattttt tctccttcca gttaaaccat gaaatcgctt ttttcaaaag
     1621 tggcagatct gatgaatcca ggtagatatc accaatatgt tttatagccc ttttttgcaa
     1681 gacactatct ttactcataa gatcttcaaa aaaaagacct gattttttta caaaaagatt
     1741 agtccctttt aatgtatcaa ccggtgtaaa tgtttcataa aatattttta caaaatcact
     1801 gggcggagtc agtgtatcag cctgggtggt aagtgaaaaa ttaataccat ttttgtaata
     1861 agtctttgtc cagatggtac ggctgctgcc tgaatctgtg agaatagctt cccagacttt
     1921 cattttaccg ggtaattcag ttgtcttaaa cagttttaca atccagctag aatcgccccg
     1981 gtaggagaca aacttatccc tgtgcaccag ggaactgtct ttactgtagt agtaacgggg
     2041 ggagcggtaa aaagaaatat atattttttc ccctgttgta tcattagcca caattttatt
     2101 cctgaaagcg ccactttcca gtaataaatc ctctgactcc ggatcctcat aatccccttc
     2161 tccacccatc cactggtagc gggggatgtc taactttatt tttttctctt ctgggaatac
     2221 gggggtaatt acactaaaat agagggtggt atccgtttct ttttttgccg cctggtaaat
     2281 aaaaggtttt atctcaaacg aattaagaaa attattcatt tccggcaatt cctgtcttcc
     2341 atgtgctatc agtgtataat aatgcggacc ctgaataata aagcgaacag cataaatgga
     2401 tccttgcttg tccctgtact ttgcttccag cgccggataa cctttataag ttgtttgaac
     2461 cctggatagt tgtgaatcaa taaattcaga cgccataaaa ctctcttcca taaggccaag
     2521 gtcgaaagta tcttcttcag caaaattgta attgtgaata tcacttcgta tcacccggta
     2581 atgagttgcg gtggtcttgt cctctgcgtc aaaaatccag ctgccatcat ttcctgtata
     2641 gggcacatat ggaaaatcaa cagtaaaccc tccgtagccc ggagaatatt ttttccagcc
     2701 cgcagaggcc tccacatcat tcttatattc cttcagttga atacttccaa aaaaacgctt
     2761 tgcttcatca cctagtttta catagtcccc attcccgctt attttaaaga atagtacttc
     2821 gaacggagtc acgaaaatat tataccgctg cagatcaccc cgccgggtac ggttggtaat
     2881 atcaaatcct ttatagccgt tttttgtaac agggatctta gcaattattt tgcccggtac
     2941 attctcatac aacaggctat ccactttctt cagtacatca tcaacagtat gattccacat
     3001 ccaggcattt gtcattattc tggtaaccat ataataactg ccattggcca tatctgcata
     3061 ttgcttttga tccaatgctg catcctcacc aaacttgtag aacttgcctg gtatatccac
     3121 tttaaaaaaa ccatcctctg ctgtttcagt atgaaaagag actggcaccc gaatcttatc
     3181 aaccatgtct ttttccctgc tggctctttc gcccattttt ataggacgca gtttatatcc
     3241 tttggcacgc aacatttcta ttactccacg ctgtccgggc aaatgagcag cccccacccc
     3301 aacaaataat acagacccgg acttaagaat tgaatcaatg gaatttgcct gtatctcatt
     3361 tcgcttgtat aaaaattttt catcgaaagc cgcagagaaa ctattgaatg tattaatgga
     3421 atctaacagg tcaaggtcac ctttacgata agcctcctgg agtctgtccg cagcatattc
     3481 ttcatccaca tcgccataac ttctgtcttt cttgttttta tctttggcag catctttata
     3541 ggcctcggcc atcagccgca tactttggcc ataatcttcc acaccagtta cttttttccc
     3601 ccacttcttg ccgcattggt agatgtacat atccaggtag gtatcttctt caaaatcact
     3661 tgattcgttc ccgtaggagc ggtaaagtaa attattgatc gtggagggat tgctgaaaag
     3721 tgccctttca atctttgcat catatttata gaactttaat gtattgatgc ccagataatc
     3781 ctccggcatg ctggtatact ccccatatcc ggcacttgca ccataatatc cttcttcgcc
     3841 caggtcgtat ttactcatat cctcttgcca tgtttccggg ttggtttcca gcgccactac
     3901 ctgtgctttt ctgattgcga tataaaaaga atccggcaag ttaaaagcaa gtttgctgct
     3961 tacatgcata gtaccaatga ggtaagaagg ctttttacca ccattgccgg ttatctccca
     4021 cagcagactg ggatattttt tttccttaat gtttgcctgc gcctgtacta aaacaaaaga
     4081 aaaagttgct acaaagaata taagaaatcg tttcataaag gctggtatcc cgacagctgc
     4141 gggcgacttt tgtaaaaaaa caaaagtaag atgccgggta gcaataaaac cataaaatta
     4201 ttttaggaac caatggcttg gacaaataac gaggagtgaa ttttcctaaa aactaacata
     4261 ctttgcacat atcactgcct ttaaaaaaat atacggtttt tagccgataa aatcttactt
     4321 ttgcccgctt taaaagcagt cgttttgacc aaacccggcg ccgtaaggcc ggctaggatc
     4381 atctctccgg cgtttgcccg gtttgcgtca tgagactttt ttgttataaa cccaaacact
     4441 catgaagctt tcacaattcc gattcgacct tccgttaaac cttattgcac aaaatccgac
     4501 caaaagacgt gaagatgccc gaatgatggt ggtccatcgt cataccgggg aaattgaaaa
     4561 caagcacttc cgtgatatta ttgactattt tgatgacaag gattgttttg tggtgaataa
     4621 taccaaggta tttcctgccc gtatgtacgg acgtaaagaa aaaaccggcg ccaagatcga
     4681 agtgttcctg ctccgtgaac tcaacaaacc aaaccgtctc tgggatgtga tcgttgatcc
     4741 cgcaagaaag atccgtgttg gcaacaagtt atatttcggc gaacaggaag acctggtagc
     4801 tgaagtaatt gataatacta ccagccgtgg acgaaccatt cgttttttat gggatggcac
     4861 tgaagaagaa ttcagggctg tgctggaaag attaggtgaa acccctttgc ctaaatacat
     4921 caaaagaaaa ccagacgaag aagataaaga acgttaccag acagtttatg ccaaacatga
     4981 aggcgctgtt gctgcaccta cagccggttt gcattttagc cgtgaattga tcaaaagatt
     5041 agagatcaaa ggtatccgtt ttgcagaagt aaccctgcat acaggactgg gtactttccg
     5101 tcctattgaa gtggaagacc tgagtaagca taaaatggat gctgaatatt acaatattga
     5161 tgaaacagcc tgtaaggtgg tcaaccgtgc aaaagaatat ggccatcgtg tttgctctgt
     5221 tggtactact accatgcggg cactggagtc ttcttttacg gcacaaaaac tattaaagcc
     5281 gtcagaagga tggacgaata tgtttattca tccgccttac gatttcagca tcgccgattc
     5341 attggttact aattttcatt taccaaaaac aagcctgctg atcatgacct gtgcgtttgc
     5401 gggttatgaa ctggcaatgg aagcctataa aaaggcgatc aaagacaaat acaggttctt
     5461 tagttatggt gatgccctgc tgataatata acttaagaat taattaaatt aaaaatgccc
     5521 tggatagccg gggcattttt aatttatgat agattatact atttaaactt caggttcatt
     5581 gatataatat ctgagttaag gccattggtc cttgtataat gtccatacct cagttcaagc
     5641 atatttagcc gctgaatgcc aaatacaccc ttcggcggta ctatccggaa acctgctcca
     5701 taaaaattac tactgaactt tgataaatca tagttactgg tataatactg gtcggcagca
     5761 gtatgcactt tatacccacc aaaaaaatca atagcagatt gagtataata acggtaaaag
     5821 ggagttacag ataaaaaggg gttgattttt attggtgttt ccaggtcaac agtatgggca
     5881 ctcaatcccc aatcatcatg gtaataacgg taaaaactcc ggagaataac cttatcacta
     5941 agaaaataat ttgcccgaaa tccaattggt attttcaacc gggtatctgg taaattttca
     6001 ataacaggca atattgctcc attaaaatat acccggtgaa aaggaaggcc gagataaccc
     6061 tgctgataga taagatcagc aatgaacatg atttgcaact gttggttaat tacctgggaa
     6121 tatgaaaaag aaccgctaaa agaatttctt gctttagacg gataatcctt cctgctctct
     6181 tcttcaccat tccccccgcc tccccctccg ccaggtctca attcaatcgg agtgatgtag
     6241 gcgaggttat caatataggc ttgtagtttc gcagaaaact caccacttct gttctttgtt
     6301 ttttgtgcaa aacttatatt ggctccaaaa gacatataat caaattcaga agaggctgaa
     6361 atgccagcgc ctaaagtgct gccttttttc tcattttcca tagaccatgc cagagaaggg
     6421 aaaattcttg tatcggcatg agaagctgaa gaaactgtaa agggatcaat tttatctgaa
     6481 gaagcagaag tataatggtc aatcccaact tccagatcaa aactatgttt cctgtctttc
     6541 ttatcccact ttgtaaactt tacatcaatg gtatttgaaa tatcagtaag cttttgcgtt
     6601 ccaattccac cggtcactgc tgcattgtta ccatcctggg cataatagct ggaaaccagg
     6661 ttggcttctt caaaagtaag tttacggctt ttatatccga ctgagtcctt gggtactgat
     6721 tgtgcaaatg aggcaaagaa agtaaggaat atgcccgca
//
LOCUS       DAORTI010000161         6686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_13216_length_6686_cov_5.844820, whole genome shotgun sequence.
ACCESSION   DAORTI010000161 DAORTI010000000
VERSION     DAORTI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13216_length_6686_cov_5.844820"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..908)
                     /gene="infC"
                     /locus_tag="PLO70_14725"
     CDS             complement(273..908)
                     /gene="infC"
                     /locus_tag="PLO70_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQZ75771.1"
                     /translation="MAVPQKPGGGFSRPPGGGGFKPGGGGFNKGGFRGRFAPRKEAEH
                     RINHFIRVPQVRLVGDNVEVGIYSTQDALKMAQDQQLDLVEISPTVDPPVCKIIDYNK
                     FLYDKKKKEKEMKAKSKVSEVKEIRFTPNTDDHDFDFKAKHAIEFLKDGNKVKAYVQF
                     KGRAIQFQDRGQLLLLKFAERLAEIGVLENMPKMEGRRMLAMFAPKGKKKA"
     gene            complement(1006..2940)
                     /gene="thrS"
                     /locus_tag="PLO70_14730"
     CDS             complement(1006..2940)
                     /gene="thrS"
                     /locus_tag="PLO70_14730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQZ75772.1"
                     /translation="MINITLPDGAVRQYEKGVSALDIAKSISEGLARKVLAASINGQV
                     WDATRPINEDASLKLLTWSDGDGKNTFWHSSAHLMAEAVETMFPGVKFWVGPPVENGF
                     YYDMDLGDKKMSEEDLVALEKKMNELAKKNSAYIRKEIPKEEAVKYFSEKGDEYKLDL
                     LSNLNDGEITFYTQGDFTDLCRGPHIPNTSFIKAIKLTSIAGAYWKGNEKNKMLTRVY
                     GVTYPSQKELDEYLAMLEEAKKRDHRKLGKELGIFTMDDDVGPGLPLWMPNGTVIIEE
                     LEKLAKETELAAGYKRVVTPHIAKESMYLTSGHLPYYADSMFPPMEMDGEKYYLKAMN
                     CPHHHKIFDAEPKSYKDLPYRLAEYGTCYRYEQSGELFGLMRVRCLHMNDAHIYCTKE
                     QFYDEFKAVNEMYLKYFKIFGIDKYVMRLSLHSPEKLGQKYVDEPELWKETEALVRDV
                     LIKTNTPFVEVQDEAAFYGPKIDVQIWSIIGREFTLATNQVDFAQGRRFKLEFTNQDN
                     KAETPLIIHRAPLGTHERFIGFLLEHYAGKFPLWLAPVQVKVLPISDKFIDYAKSVSD
                     KLKKADIRSEVDDRNEKIGKKIRDTELMRVPYMLVVGEKEMNENKVAVRRQGKGDMGV
                     KTVDEFLTNAIEEIKDRREE"
     gene            3249..4331
                     /locus_tag="PLO70_14735"
     CDS             3249..4331
                     /locus_tag="PLO70_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75773.1"
                     /translation="MKRVIYRYLLSFFVVLSITGSNSIAQNLTGIWRGYFITESYEHY
                     KFELQVKQTGNAVSGVSYSYLSTVFYGKATLTGNYNKAGQKALIQEIRTVELRMSGGS
                     QACIMKCIFEYSRSGKEEFLEGTYSSKFEKDGLITKKGDNCGGGKVYLRKVTTSDFYI
                     EPFLRGKIKTTPVIVNKPPVKTNPKPTTPVIVNKPPVKKPVVANKPPVKKDTVKIKPV
                     LPPLVKVDSTTKKIKPVVVTPTPPVIRNRTNELVKVLTVNSPDVVVKLYDNGEIDGDT
                     ISVYLDKKLVLSEKRLTASPLVLNFKMDEDNNEHELTMVAENLGKIPPNTSLMIVESG
                     GQRFEVRITSTEQKNAVVRFKYQSVR"
     gene            4425..5291
                     /locus_tag="PLO70_14740"
     CDS             4425..5291
                     /locus_tag="PLO70_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75774.1"
                     /translation="MPRQIIIVVMLLSGLFTEAQDINGIWKGKIVMAPSGCFPVYNIE
                     LQLQVAGTRITGTAYHFSDSLNYIKENFEGNYNRDSNQVIIKEIGIVTFKIREDCVPC
                     IKTYTLTYHRGGGNVVTEEQLRGSWATPSGKAIDGKTICEPGTIVLTRFEKSSFKPEL
                     KLPPSLTKRKAELVKEIKVDTGSIKIDFYDNGQIDGDTISVYVNNMPVVSNRMLKTQP
                     ISVSIKIDNIRTMQEVIMVGENLGSIPPNTALMIVTAGNKRYQLYLTSDEQKNAMVRF
                     LYEKPVMASKNN"
     gene            complement(5562..>6686)
                     /locus_tag="PLO70_14745"
     CDS             complement(5562..>6686)
                     /locus_tag="PLO70_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75775.1"
                     /translation="KFIPNVVTGRQDSIEFRHNNEGAKYAAETALYLQDDWELGEKWK
                     INYGIRWSSFTQIGPYTKYIRDADGNKLDSTIYKNFDPVKTYSGLEPRITIRYAINDE
                     TSVKTAVTRNYQYIHLVTNAGTTLPTDLWVPSTYIVRPQVSWLYSAGIFKNFSDNAFE
                     TSLELYYKDMKNQVEYKEGYTPSLADPEDEFVFGRGWSYGAEMLINKLKGRWTGWVGY
                     TLSWTWRKFPQLNEGEKYPARYDRRHDLSVVGNFEASRKWKLGAVFVYGTGNAITLPE
                     RFYIINGVLTQEFSKLNQYRMKSYHRMDVSATYTPIPKKKRKVSSYWVFSIYNVYSRL
                     NPYFLYFDQEGSATNGDLNIETKQVSLFPILPSVTWNFKF"
BASE COUNT         1927 a         1408 c         1264 g         2087 t
ORIGIN      
        1 cgtgtagtat ccggccgggt gttactgggt tgttgtgcag tggcagcaat tgaaatgata
       61 gtaaaaaaca aaagagaaat taagcggcga aatgataatg gcataaatat cagttttaag
      121 tggctcaaga tactgaaagg agcggatcag taacgttgaa tgttaatttt tatacgaacg
      181 gttattacac agtgatgaac aattaaaaaa caactacaat aaagaacccg tcacgcaatg
      241 cggacgggtt catataaaat agaaatttaa tactatgctt tctttttccc ttttggagca
      301 aacattgcca gcattcttct tccctccatc tttggcatat tttcaagtac tcctatttca
      361 gcaaggcgtt ctgcaaattt taatagcagc agttgacctc tgtcctggaa ctggatagca
      421 cggcctttga attgcacata cgcttttact ttgttgccat ctttcaaaaa ttcgatagca
      481 tgttttgcct taaagtcaaa atcatgatca tcggtattag gtgtgaaacg tatttctttc
      541 acctcagaga ctttgctttt ggctttcatc tctttttcct tcttcttctt atcataaagg
      601 aatttgttgt agtcaatgat cttgcaaacc ggggggtcga ctgttggtga aatttcaaca
      661 aggtcaagtt gttggtcctg cgccatcttt aaggcatcct gtgtactata gatacctacc
      721 tcaacattat cgccaaccag cctcacctgt ggcacacgga taaaatgatt gatacgatgt
      781 tctgcctctt tacggggggc aaacctgccc ctgaaaccgc ctttgttgaa tccgccaccg
      841 ccgggtttaa aacctccgcc gccaggtggc ctgctgaacc cgcctccggg tttttgtggt
      901 actgccattt acttttatta agtgaagcct ccagcccctg cagggaagca tatttttttt
      961 aaattattaa attcccgtcc aggaaacggg atactaaaat acattttatt cttctctgcg
     1021 atctttaatt tcttctatcg catttgtcaa aaactcatca actgttttta cacccatatc
     1081 ccctttcccc tgtctcctaa cggccacttt gttctcgttc atttcttttt cacccactac
     1141 aagcatgtaa ggaactctca ttaattctgt atcacggatc tttttaccaa ttttttcgtt
     1201 tctgtcatca acctcgctac gaatatcagc ttttttcagt ttatctgata cagatttggc
     1261 atagtcaatg aatttgtcac tgatggggag cactttaacc tgaaccggtg ccagccaaag
     1321 tggaaacttg ccagcataat gttccagtaa aaagccaatg aaccgctcat gggtgcccaa
     1381 cggggcccgg tgaattatca gtggggtttc agccttatta tcctggttgg taaattccag
     1441 tttaaatctc cggccctggg caaaatccac ctggttggtc gccagtgtaa attcccggcc
     1501 aatgatgctc catatctgta cgtcaatctt cggcccataa aaagcggcct catcctgcac
     1561 ctctacaaac ggagtattgg tttttattaa tacatctctt accaatgcct ctgtctcttt
     1621 ccacaattca ggttcatcta catatttctg accaagcttt tcaggtgaat gcaaagagag
     1681 tctcattaca tatttatcaa tcccgaaaat tttaaaatac ttgaggtaca tttcattcac
     1741 tgctttgaac tcatcgtaga attgttcttt ggtgcagtaa atatgtgcat cattcatgtg
     1801 caggcagcga accctcatca atccaaataa ttcaccactc tgctcataac gataacaggt
     1861 tccgtattcc gctaaacgat aaggaagatc tttgtagctt ttaggttctg catcaaatat
     1921 tttgtggtga tgcggacagt tcattgcttt caggtaatat ttctccccat ccatttccat
     1981 cggcggaaac atactgtcgg cataataagg caggtgacca ctggtgaggt acatgctttc
     2041 ctttgcaata tggggagtaa ccaccctttt gtatcctgct gcgagttctg tttcttttgc
     2101 cagtttttcc agctcctcaa taatcactgt tccatttggc atccataaag gaagaccagg
     2161 cccgacatca tcatccattg tgaagatgcc taattcttta cccagtttcc ggtgatccct
     2221 tttctttgct tcttccagca ttgccaggta ctcatccagc tctttttgcg aaggataagt
     2281 aacaccataa accctggtca acatcttatt tttctcatta cctttccaat aagcaccagc
     2341 gatactggtt aattttattg ctttgatgaa cgatgtattg ggaatatgcg gtccccggca
     2401 cagatcagta aaatcaccct gtgtataaaa agttatttcc ccatcattaa gattactcag
     2461 caaatcaagt ttatattcat ctcctttttc gctaaagtat tttacagcct cttcctttgg
     2521 tatttctttt cttatgtagg cactgttttt tttggccaat tcattcatct tcttttcaag
     2581 ggccaccagg tcttcttcac tcattttttt atcacccaag tccatgtcat aataaaaacc
     2641 attttctacc ggaggcccca cccaaaattt aaccccgggg aacatcgttt ctaccgcttc
     2701 agccatcagg tgagcggaag aatgccagaa tgtatttttt ccatccccgt cactccatgt
     2761 taatagtttt aaagaagcat cttcattaat gggtcttgtt gcatcccaca cctgtccgtt
     2821 tatagaagca gccaggactt ttctggccaa cccctctgat atagattttg ctatgtccag
     2881 ggctgacaca cctttttcat attgcctgac ggcgccatct ggtagagtaa tattgatcat
     2941 ctgtaaaacc cggtttttat cggagtgcaa agctaacgaa ataaggctaa agatgatgca
     3001 gttttaagcg gctcaaggag ttccctttct tgtttaaatt ctccaataaa tggataaaaa
     3061 gggttaatag gaaaaggaaa aaagtgccag ctattgagta aacccggctc gctaaaataa
     3121 gttaagtatt atacggaaca ggatacattt taacagcttc tgctatatta aaagctgtgt
     3181 taaattaaca aactattgct ccgggcaaac tccgtgtata aaaacagttt cagtagtttt
     3241 gccacttgat gaaaagagta atatacaggt acctgctgtc atttttcgtg gttctatcca
     3301 ttaccgggtc caattctatt gcgcaaaacc taaccggtat atggcggggt tattttatta
     3361 cggagtccta cgaacattac aaatttgagc tccaggtcaa gcaaacagga aatgcggttt
     3421 caggtgtttc gtattcttat ctctccactg ttttttatgg caaagcaact ctcaccggaa
     3481 attataataa agcaggtcag aaagcattga tccaggaaat aaggacagtt gaacttcgta
     3541 tgtccggcgg atcgcaagcc tgtattatga aatgtatttt tgaatacagc cgttccggta
     3601 aagaagagtt tctggaagga acctattcaa gtaaatttga aaaagacggc ttaattacaa
     3661 aaaagggaga taattgtggc ggcggtaaag tttatctcag aaaagtaact acatctgatt
     3721 tctatattga accttttttg aggggtaaaa taaaaacaac acctgttatt gttaataaac
     3781 cgccggtaaa aacaaacccc aagccaacga ctccggtgat tgtaaacaaa cctccggtaa
     3841 aaaagcctgt agtggccaat aaacccccgg tgaagaaaga tactgtgaag atcaaacctg
     3901 ttttaccacc gttggtaaag gtggatagta caaccaaaaa gataaaacca gttgttgtta
     3961 cacccacccc acctgttatc aggaacagga caaacgaact ggtaaaagtg cttaccgtaa
     4021 atagccctga tgtggttgta aaattgtacg ataacggtga aattgatggc gatactattt
     4081 ccgtttatct tgacaagaaa ttagttcttt cagaaaagag actaactgct tctcccctgg
     4141 tgctcaactt taaaatggat gaggacaata acgaacatga attaacgatg gtggcagaaa
     4201 acctcggtaa aataccgcca aatacttctt tgatgattgt ggaatcaggc gggcaacgtt
     4261 ttgaggttcg gattacgtct acagaacaga aaaatgccgt agtgagattc aaataccagt
     4321 cagtaaggta agcgttatct tccattttgc agatcaggtt atattattgc cgttgctgta
     4381 attttttatt tctcttggtt attatcattt atttttccac cagaatgcca cgccagatca
     4441 tcatagttgt tatgctttta tcgggccttt ttacggaggc acaggatatc aatggtatct
     4501 ggaaaggtaa aattgtaatg gcgccaagtg gttgtttccc tgtctataat atcgaattac
     4561 aactccaggt ggccggaacc cgaataacgg gaacagctta tcatttttct gattcactta
     4621 attacattaa agaaaatttt gaaggcaatt ataacaggga tagtaaccag gttattatta
     4681 aagaaattgg cattgttacg tttaaaataa gagaggactg tgtcccctgt atcaaaactt
     4741 acactctcac ctatcatcgg ggaggcggaa atgtagttac ggaggaacaa ctaaggggta
     4801 gttgggctac ccccagtggc aaggccattg atggtaaaac catttgcgaa cccggtacaa
     4861 ttgtattgac caggtttgaa aaatcttctt ttaaaccaga attaaagttg cctccctcgt
     4921 taactaaaag aaaggcggaa ctggtaaaag aaataaaagt ggatacgggt tctattaaga
     4981 ttgattttta tgataatggc cagattgatg gagacaccat ttcagtgtac gtaaataata
     5041 tgcctgtcgt ttcaaacagg atgttgaaaa cacaaccaat aagtgtttcg atcaaaatag
     5101 ataatatccg gactatgcag gaagtgataa tggtaggtga aaacctgggt tctatccctc
     5161 ccaatactgc cctgatgatt gtaactgcag gcaataagcg ctaccagctt tatcttactt
     5221 ctgatgaaca gaaaaatgca atggtgcgtt ttttatacga aaagccggtt atggcttcaa
     5281 aaaataatta acaatttttg ggttgatcat atagaaacag acttttgatt atttcgcttt
     5341 ttgcaaacaa tgatcaataa acgcaaacaa ccgtttgctt aatgaatcgg cctctaattg
     5401 taactactta attgatgaaa tcaatcctgc aaaaattgtt ttatgttttg atgaatgatg
     5461 tgtaataact attttcacat cgcaaacaat ttttaatctc aagttatttg acacaaaaag
     5521 cctgcttctg caggcttttt tatttttttt aaaaccgatg attaaaattt aaaattccag
     5581 gttacagagg gtaaaatagg gaatagggaa acctgtttgg tttcaatatt taaatctcca
     5641 ttggttgcac taccctcctg gtcgaaataa aggaaatagg gattgaggcg gctatacaca
     5701 ttataaatac taaacaccca ataactactg acttttcttt ttttctttgg tataggagtg
     5761 taggtagccg atacatccat ccggtgatag gatttcatcc ggtattgatt gagcttactg
     5821 aattcttggg ttagtacacc attgataata taaaaacgct ccggtaatgt tattgcattg
     5881 cctgttccat atacgaatac agcacccagc ttccacttcc tgcttgcctc aaaatttcca
     5941 acaacagaaa gatcatgacg cctgtcatac cgtgccggat acttctctcc ttcatttaat
     6001 tgaggaaatt ttctccaggt ccaggacagg gtatagccta cccatccggt ccatctgcct
     6061 tttaatttat ttatcagcat ttcagcgcca tagctccagc ctcttccaaa tacaaattcg
     6121 tcttccggat cggctaatga aggagtatag ccttccttgt actcaacctg gtttttcatg
     6181 tccttataat acaattccag ggaagtctcg aatgcgttat ctgaaaagtt tttaaaaatc
     6241 ccggcagaat aaagccagct tacctgtggc cttacaatat aagtactggg tacccatagg
     6301 tctgttggca atgtggtacc cgcattggtt accaggtgta tgtattgata gttccgggta
     6361 acagctgttt ttacagaagt ttcatcattg atggcatagc gtatcgtaat acgtggttct
     6421 aaaccactgt aagtctttac aggatcaaaa tttttgtaga tcgtgctatc cagtttatta
     6481 ccatcagcat cacggatata ttttgtatag ggacctatct gggtgaagct gctccacctg
     6541 atgccataat ttatcttcca tttttctccc agttcccagt catcctgcag ataaagtgct
     6601 gtttcagccg catatttagc cccttcatta ttgtgtcgga attcaatcga atcctgccgg
     6661 ccggtaacta catttggaat aaactt
//
LOCUS       DAORTI010000162         6608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_13468_length_6608_cov_6.147719, whole genome shotgun sequence.
ACCESSION   DAORTI010000162 DAORTI010000000
VERSION     DAORTI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_6608_cov_6.147719"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1146
                     /gene="pdhA"
                     /locus_tag="PLO70_14750"
     CDS             151..1146
                     /gene="pdhA"
                     /locus_tag="PLO70_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQZ75776.1"
                     /translation="MAAKFSKETYLYWYELMQLIRQFELMAEEKYKMEGKIRGFFHAY
                     IGQEAIAAGCMTATRPEDLYITAYRDHGLAIAKGVSVNSCMAELYGKATGCAKGKGGS
                     MHFFSKEANFYGGHGIVGAQIGTGAGLAFAEKYRGSDNVVLTYFGDGAARQGILHETF
                     NLAMLWKLPVVFICENNNYAMGTSVERTSNVIDIYKLADAYEMPADTIDGMSPEAVHE
                     GVGRAVKRAREKGGPTLLEIKTYRYKGHSISDPQKYRTKEEVDEYKGKDPIQQVLKTI
                     LDNKYATQEEIDAINKRVDAIVEESVKFAEESPWPDDSEVLKDVYVDQNYPFIVD"
     gene            1179..1871
                     /locus_tag="PLO70_14755"
     CDS             1179..1871
                     /locus_tag="PLO70_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQZ75777.1"
                     /translation="MAENKNVQDMDSSEVVIAKAKSFWDKYSKPLMIVSTLIILVIGG
                     WYVYQNFFQKPKEAKAIEAMFRAEEYYRLDSVNLALNGDGQNLGFVKIVDKFGGTKAG
                     KLASFYAGSCYLKLNDYEKAVKYLKKFSSSSDLVQARAYKLLGDAYADWGKNDEALSN
                     YKKAGRHFEKDEANSPEYLFAAAYFADKVMKNQKEAISLYKELKEKYPRSQQAFDADN
                     YLAQLGVYSTEN"
     gene            1874..2371
                     /gene="ribH"
                     /locus_tag="PLO70_14760"
     CDS             1874..2371
                     /gene="ribH"
                     /locus_tag="PLO70_14760"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQZ75778.1"
                     /translation="MADITNSKLLHTDTGILNKDACIVLVKTEWNAAIVDQLERGCIA
                     ILEQHAVKKIIILTVPGAVEIPFAIKSFWENTGKKKRVDAFIALGCVIKGDTPHFEYV
                     CKAVTDGVGTLNLTMPVPTIFGVLTVNDEQQAMERIGGKHGHKGEEAAMTALKMISLL
                     KSLKK"
     gene            2375..3094
                     /locus_tag="PLO70_14765"
     CDS             2375..3094
                     /locus_tag="PLO70_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQZ75779.1"
                     /translation="MNVQLFIPCFVDQLYPDTAFNMVKVLEKAGCTVSYNTSQTCCGQ
                     PAFNAGFWDEAKSVCTKFLKDFSGTDLIVAPSASCVGFVRNYYSKLFENSSHHHAVQD
                     IGKRIFEFSEFMLQVLKIEDVGAVLNGKATYHDSCAGLRECKIKQEPRKLLAHVKGLT
                     LTEMNDVETCCGFGGTFAVKFEAISIGMGEQKVENALATGADYLISTDISCLMHLQGY
                     IKHKGYSLKTMHLADVLASGW"
     gene            complement(3140..3319)
                     /locus_tag="PLO70_14770"
     CDS             complement(3140..3319)
                     /locus_tag="PLO70_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75780.1"
                     /translation="MKHTQSASKIKPVDNLLNELYLKRNTLYYDPNNPGKYSIYQMNL
                     RTGTVPENLPRRIRQ"
     gene            complement(3334..4020)
                     /locus_tag="PLO70_14775"
     CDS             complement(3334..4020)
                     /locus_tag="PLO70_14775"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQZ75781.1"
                     /translation="MKFKSSTTTCFLLLSFISTVAQVNDTTLSKVLPFTNEKSILKII
                     LESDSSTKSVFVSNLKEDKAGDMSPVIGAANWMDNGKELLCRSLLEFNYNLIPVEILD
                     NPLLILQAELVLYPIHAEFAVNDNAKTSVIHIRQVLDKWEDSSTTWNSQPKTNFENQS
                     SVTIETKNKDIPASTDVTDIVLNNIIKGKNNGFMIVTDENRNKSFSAGQLFASPKMKI
                     RVFVLSWFYM"
     gene            complement(4153..4722)
                     /locus_tag="PLO70_14780"
     CDS             complement(4153..4722)
                     /locus_tag="PLO70_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQZ75782.1"
                     /translation="MKQIKFAGLMLAAILSMNSAFAQGNRMKEKTVEVGGAAMYPSKN
                     IVENAVNSKDHTTLVAAVKAAGLVETLQTAGPFTVFAPTNDAFGMLPAGTVEALVMPE
                     NKARLTSILTYHVVAGRLGTQELDELIKKGKGVAELVTVAGGKLWIIKKDAKYWLKDE
                     NGGMAQLTISNVYQSNGVIHVIDHVVLPK"
     gene            4787..5296
                     /locus_tag="PLO70_14785"
     CDS             4787..5296
                     /locus_tag="PLO70_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQZ75783.1"
                     /translation="MNRKRYGGGDGFGLALIKFLSNFHYICFYNNLDMAYWLIKSEPF
                     KYSWEMFEKDKQTFWDGVRNYAARNNLKGMKKGDLAFFYHSNEGTEIVGIAKVAKEAY
                     QDPTTDDAAWVAVDFKPHKKLKKPVGLAQIKADKRLASMALVRLGRLSVQPVTEEEWD
                     IVMKMAEEQ"
     gene            5421..5816
                     /locus_tag="PLO70_14790"
     CDS             5421..5816
                     /locus_tag="PLO70_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75784.1"
                     /translation="MNKLIIGLLIIAAGAGVYFLLSKKKHSTETADIKKELIIGTWKV
                     ESYLPATDSLQPKYVYDFQKDGMAVRSVNDKINTDTVKYEWNKISELVIKENITDSTG
                     KVYTVEKLTKDSLQIIGTDSVVVTLTRAK"
     gene            5898..>6608
                     /locus_tag="PLO70_14795"
     CDS             5898..>6608
                     /locus_tag="PLO70_14795"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQZ75785.1"
                     /translation="MKYFKLFSIILLVLSIQSCENKATNAGKYANAALPVPDEEPSYQ
                     NYIADSAASPQKEEKQQQPKEKKQPIVNAEWDKKIIKTALLNLEVKDYTGYYSSLREK
                     IKNIGGYIAQEEQRQSAYKIENTLVIKVPVEQFDNALVQLTDKAEKLNEKKITSQDVS
                     AEYIDIRSRMESKKQVRQQYIALLKQAKNMEDILNVQSEINSIQEEIEAAAGRVEYLG
                     HSSAYSTINLTYYQVLNSS"
BASE COUNT         2164 a         1181 c         1401 g         1862 t
ORIGIN      
        1 aaaacagata atatttgaat agcttaaaga tctttattgt attcatcaaa ggaaacagta
       61 aacatggaat caaaaccgga ttaccctctc aaaatcactg ctttaaaatt ccttttttcc
      121 ccttattttt gcgactcaaa atttaaacct gtggccgcca aattttctaa agaaacttac
      181 ctctattggt acgaattgat gcagctgatc cgccagtttg aactgatggc tgaagagaag
      241 tataaaatgg agggcaagat ccgtggcttt ttccatgctt atatcggcca ggaagctatc
      301 gcagccggtt gtatgactgc cacccgtccg gaagatttat atatcacagc ttatcgtgac
      361 catggattgg ctattgccaa aggtgtatct gttaacagtt gtatggcaga attatatggt
      421 aaagcaacag gttgtgcaaa aggaaaaggt ggaagtatgc acttcttcag caaagaggcg
      481 aatttttatg gcggacatgg cattgttggt gcccagattg gtacaggagc agggttagct
      541 tttgctgaaa aataccgtgg ttctgacaac gtggtactca cttattttgg tgatggtgct
      601 gcacgccagg gtatattgca tgaaactttt aacctggcca tgttatggaa attgccggtg
      661 gtttttattt gtgaaaataa caactatgca atgggtactt ccgttgagcg tacctctaac
      721 gtaattgata tttataaact ggcagatgcg tacgaaatgc ctgccgatac tatagatggc
      781 atgagtccgg aagccgtaca tgaaggagtg ggcagggcag taaaaagagc aagagaaaag
      841 ggtggtccga ctttactgga aataaaaacg tatcgttaca aaggccattc tatcagtgac
      901 ccgcagaaat acaggactaa agaggaagtg gatgagtata aaggtaaaga tccgatacaa
      961 caagtattga agaccatcct ggacaacaaa tatgcaacac aggaggagat tgatgcaatc
     1021 aataaaaggg tggatgccat cgtagaagaa tctgtaaagt ttgcagaaga aagcccatgg
     1081 ccggatgaca gcgaggtact gaaagatgta tatgttgacc agaactatcc ttttatcgtt
     1141 gattaaatat tacaaataaa acaagtaaca taaattttat ggcagagaac aaaaatgtgc
     1201 aggacatgga tagcagtgaa gtggtgatcg caaaagcaaa gagcttttgg gataagtaca
     1261 gcaaaccact gatgatcgtt tctacactta ttatcctggt tattggcggc tggtatgtgt
     1321 accagaattt tttccaaaaa ccaaaagaag caaaagctat tgaagcgatg ttcagggcag
     1381 aagaatatta tcgcctggat tctgtaaacc tggcactgaa tggtgacggg caaaatcttg
     1441 gttttgttaa gatcgttgac aaatttggcg gaacaaaagc cggtaaactg gcttcattct
     1501 atgccggcag ctgctacctg aaacttaatg attatgaaaa agcagtgaag tacctgaaaa
     1561 aattcagcag tagttccgac ctggtacagg caagagcata caaactcctg ggtgatgctt
     1621 atgctgattg gggaaagaat gatgaggcat taagtaatta taaaaaagcg ggccgtcatt
     1681 tcgagaaaga cgaagcaaat tcaccggaat acctttttgc agcggcatac tttgcagata
     1741 aagttatgaa gaatcagaaa gaagctattt cgctttacaa ggagttgaaa gaaaaatatc
     1801 cccgcagcca gcaagctttt gatgctgaca actaccttgc tcaattgggt gtttacagca
     1861 ctgaaaatta atcatggcag acattaccaa tagtaaacta ttacatactg atacaggcat
     1921 tctgaataag gatgcctgta tcgttttggt gaaaactgaa tggaatgccg ccattgttga
     1981 tcaactggag agaggttgta ttgccatcct tgagcaacat gccgttaaga aaataattat
     2041 cctaactgtc cccggtgctg tagagatccc gtttgccata aaaagctttt gggagaatac
     2101 cgggaagaaa aaaagagtgg atgcatttat tgctttgggc tgtgtgataa aaggggatac
     2161 ccctcatttt gagtatgtat gtaaagccgt aaccgatgga gtagggacac taaatctaac
     2221 tatgcccgta cccactatat ttggtgtatt gacagtaaat gatgaacaac aggcaatgga
     2281 aagaatcggt gggaaacacg gacataaagg agaggaagca gcaatgaccg cattaaaaat
     2341 gattagttta ctgaaatcgt taaagaaata agtattgaac gtccagttat tcataccatg
     2401 ttttgttgac cagctttatc cagatactgc tttcaatatg gtgaaagtgc tggaaaaagc
     2461 tggttgtact gtttcctata atacatccca gacctgttgt ggccaaccgg ctttcaatgc
     2521 cggtttctgg gatgaagcaa aatctgtctg tactaaattt ctgaaagact ttagtggaac
     2581 ggatcttatc gtagcgccca gtgccagctg cgtgggcttt gtcagaaact attacagcaa
     2641 attatttgag aactcatcac atcatcatgc ggtacaggat ataggcaaaa ggatttttga
     2701 attttcggag ttcatgcttc aggtattgaa aatagaagat gtaggtgctg tattaaacgg
     2761 taaagccact tatcatgatt cctgtgcagg gcttagagaa tgtaagataa agcaggaacc
     2821 aaggaaatta ttagcacatg taaaagggct tacccttacc gaaatgaatg atgtggaaac
     2881 ctgttgcggt ttcggtggca cttttgctgt aaagtttgaa gctatttcaa tcggtatggg
     2941 tgagcagaaa gtagagaatg cattagcaac aggggccgat tatcttattt ccacagatat
     3001 ttcttgcctg atgcacctgc agggttatat caaacacaaa ggctattcgc tgaagacgat
     3061 gcacctggca gatgtattag ccagcggatg gtaagttctt ctgataattt tttttcaata
     3121 cagaattcaa ctttccaagt tattgtctga ttcttcttgg aagattttcc ggtacagtac
     3181 ccgttcttaa attcatttga tatatagagt atttaccagg attattagga tcataataca
     3241 aagtattgcg tttcaggtaa agctcattaa gtaaattatc tactggtttt attttagaag
     3301 cactttgcgt atgcttcata tcctgttgat aggctacata taaaaccagc tgaggacgaa
     3361 tacccggatt ttcattttag gcgaggcgaa taattggcct gccgaaaaag atttattacg
     3421 attttcatcg gtaacaatca tgaacccgtt attcttgcct ttaattatat tatttaaaac
     3481 aatatcagtt acatctgtac tggcgggaat atctttgttt tttgtttcta ttgttactga
     3541 tgactggttt tcaaagtttg ttttgggttg agaattccag gttgttgagg aatcctccca
     3601 cttatctaat acctgcctga tatgaatgac actggttttt gcattatcat taacagcaaa
     3661 ttcagcatga ataggatata ataccagttc agcctgcaaa atgagtaatg ggttatcaag
     3721 tatttcaaca ggtattaaat tataattgaa ttcaagcaaa gaacggcaga gcaattcttt
     3781 gccgttatcc atccagttag cggctccgat aactggtgac atatcgcctg ctttatcttc
     3841 ttttaaatta cttacaaata cactttttgt agacgaatca ctctccagaa taattttaag
     3901 tatacttttt tcattagtaa aaggtagtac tttagataaa gttgtatcat tgacctgagc
     3961 tacagttgaa ataaatgaaa gtaacaaaaa acatgtagtt gtacttgact taaatttcat
     4021 ggtggaaggt ttagttatta aatataacta atttttaaaa tatatcacac ctacgtttca
     4081 gttaaaaatg gtctgaaaca aaaaatcctc cgagaggtat cgaaggagtt tttcaaagtg
     4141 gattagcaac acttatttgg gaagaactac atgatcaatt acatggataa cgccattgct
     4201 ctggtaaaca ttgctgattg tgagctgggc cataccgccg ttctcatctt tcagccagta
     4261 tttagcatct ttttttataa tccacaactt tcctcctgca acagtcacta attcagcaac
     4321 gcctttcccc tttttgatca attcatccag ttcttgtgtt ccaagacgac cagctacaac
     4381 atgataagtg aggatagatg taagcctggc tttattttcc ggcattacca gtgcttctac
     4441 tgttcctgcc ggcagcatac caaaggcgtc attagtgggg gcaaatactg tgaaaggacc
     4501 tgctgtttgt aatgtctcaa ccagaccggc agcttttact gctgccacca gggtggtatg
     4561 atcctttgaa ttaacagcat tctcaacaat attcttcgaa ggatacatag cagcgccacc
     4621 tacttccacg gttttttcct tcattctatt tccctgggca aaggcactat tcatagataa
     4681 aatagcagca agcatcaggc ctgcaaattt gatttgtttc ataattgctt tgttttaaaa
     4741 attgatgtta tgatgtatat aaaagggtaa gggcttttga acatgcatga acagaaagag
     4801 atacggaggt ggtgatggat ttggattggc tttaataaaa tttttatcca acttccacta
     4861 catttgtttt tataataatc tggacatggc atactggctg atcaaatcgg aaccgttcaa
     4921 gtattcgtgg gaaatgtttg aaaaggataa acaaactttt tgggatggtg tacgcaacta
     4981 tgctgcccga aataatctga aaggaatgaa aaaaggtgac ctggcattct tttatcacag
     5041 taatgaaggg actgagattg ttggtatcgc taaagtcgcc aaagaagcat accaggatcc
     5101 cactacagat gatgctgcct gggtagccgt cgattttaaa ccgcacaaaa aattaaagaa
     5161 acctgttggg ctggcgcaaa ttaaagcgga caaacgttta gccagtatgg cattggtaag
     5221 attaggccgc ctgtctgttc agccggttac agaggaggaa tgggatattg tgatgaaaat
     5281 ggcagaagaa caatgaatca ttaatcagaa gtcaacagtt ctgctgctaa atccccttta
     5341 acaaattgca tgggttactt ttttctgatt ggaggaatca ataactgatt ctcaccttta
     5401 aacatttaaa aagtatagcc atgaacaaac ttatcatcgg gctgctcatt atagcagccg
     5461 gagcaggagt ttattttctg ctcagcaaaa aaaagcacag tacagaaaca gcagacatca
     5521 aaaaagaact cattattggc acatggaaag ttgaaagcta tctaccggcc acagacagct
     5581 tacaaccaaa gtatgtttat gatttccaaa aagatggaat ggctgtaaga tcagtaaatg
     5641 acaagatcaa tactgataca gtaaagtatg aatggaacaa aataagtgag ttggtaataa
     5701 aagaaaatat tactgactca acaggcaaag tgtacaccgt ggaaaagctg acaaaagaca
     5761 gcttgcagat aatcggtaca gacagtgttg tagtgacact gacaagagca aaatagtaca
     5821 atgccccgcc tttaaagcgg ggcattgatt tatgcaaaag aatcctttct gcatctgtaa
     5881 ttcataaaaa caaagccatg aaatatttta aattattttc aatcatacta ctggtcctct
     5941 ccatacagtc ttgcgaaaat aaagcaacaa atgccggtaa atatgcgaat gcagcgttac
     6001 ccgtaccgga tgaagaaccg tcttatcaga actatatagc tgattctgca gccagcccac
     6061 aaaaagaaga aaaacaacag caaccaaaag aaaagaaaca gccgatagtt aatgctgaat
     6121 gggataagaa gatcattaaa acagctttac ttaacctgga agttaaagac tacaccggtt
     6181 attattcttc acttcgtgaa aaaataaaaa atatcggtgg atacattgca caggaggagc
     6241 agcgtcaatc agcgtacaaa attgaaaaca ccctggttat taaagtaccg gttgaacagt
     6301 ttgataatgc cctggttcaa ctgacagata aagcagaaaa gctcaatgaa aagaaaatca
     6361 cttcgcagga tgtaagtgct gaatacattg acatacgatc aagaatggaa tcgaagaaac
     6421 aggttcgcca gcagtatatc gcattattga aacaggctaa gaatatggaa gatatcctga
     6481 atgttcaatc agagatcaac agcatacaag aggaaataga agctgccgct ggcagagttg
     6541 aatacctggg gcattcttcc gcttacagta caataaacct tacttattac caggtattaa
     6601 attcttct
//
LOCUS       DAORTI010000163         6589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_13522_length_6589_cov_8.645393, whole genome shotgun sequence.
ACCESSION   DAORTI010000163 DAORTI010000000
VERSION     DAORTI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13522_length_6589_cov_8.645393"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1232)
                     /locus_tag="PLO70_14800"
     CDS             complement(<1..>1232)
                     /locus_tag="PLO70_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75786.1"
                     /translation="TTTLTVTSAGLVYNGNLFRCVVQNICGPTNSNSASLTVNPIPTV
                     NSGPSGLCAPVTLTASGNADTYAWTPSGGLNTTTGTTVIATPTVTTVYTVTGTITATG
                     CQNSASVTVLGTPATPAITPAAPVICAGTIQPLTVAPTTFTVTYSGAALTIPGTGTGA
                     GTGAPASVYPAVVNISGLPTSGVRVKSIAINGLSHTFPSDIDMVVQSPTGTNVVIMSD
                     AGGGTDIVNGNLVFDDAAASLLPATIVSGTYRPTNAAGPDAFPAPGPGSITQVNPPLS
                     NFTGNFNGNWNLFVVDDLGGDAGSLTSWTITFEVPTAIWSPATGLYSDPAATVPYVAG
                     TAASTVYFLQSPAVTTNYTYTVTNTLGTCSSGPASVTVTVNPVPTIAVGPNNQCGPVT
                     LTATGNSNSYSWSPAAGL"
     assembly_gap    1233..1332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1333..4628)
                     /locus_tag="PLO70_14805"
     CDS             complement(<1333..4628)
                     /locus_tag="PLO70_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HQZ75787.1"
                     /translation="MRKVLLFIGLLICAFTSFAQVDNKTKNAALQLVRQNAAAIKLPS
                     QDINDVIVSSSYQITGTDVIMVYLQQSYKGIPVYNQLKTLAFRGGKVVSDAGELQPSM
                     EKLTGNASNLPGISPANALQSAFREAKVEATELAVPISVLEDGRKFEFGKLGVSAENI
                     SAELIWMPNARGEYRLAWQVFVAPVSTSDYWLIRVDAQTGTILNKDNLTITCKWDHEN
                     HSIEKHVAEKHNKKSATSSMNYVMQRNDKSNKWEYKPFVIGTATYRVVKYPAESPQHP
                     GGTPSLHTDPWTLTPGNATSLKWHSDPADYNITRGNNVWAQEDRDANNTTFGITGNST
                     TPLPNLTFDYTYDFTKSPIDPTSDNQQAAIVNLFYMNNLMHDMAYTYGFDEVAGNFQN
                     DNQGRGGAGVDYVIADAQDASGTNNANFATPADGSRPRMQMFLWTAPNPDRDGDLDNG
                     IVSHEYGHGISNRFTGGPNVVNCLQNAEQGGEGWSDYMSLMMGTNWATATINDGPIRR
                     GIGTYALNQPITGNGIRAQPYSTDMSIFSLTYANLPGQAVPHGVGTIWCGMLWEMTWE
                     IIKQDNFIEPNFMSPPGAVSTWRGNAAAMKLVMEGMRLQPCSPGFVQSRNAILQADQV
                     FFGGRYACSIWKAFAKRGLGRNASQGSNTSISDGVPDFSVDSGNFGLNPSGPSVFEGT
                     PLTYTNVVSAGECTPMANYVLRDTLPTTVTWVSGGVYTPGDRVVTFSSINLAINTSQN
                     FPVTVNVNAGTYFAPVEHINDPVTAIAPNWTATSTSASVWATSGASVRSAPLAFFSPN
                     PAASSQQILTTTNPIVLTANPSAITSLSFWHRYVTESGFDGCVVEISTNSGGTWTDLG
                     PYMSGARYNGTLSALNTFLGGRQAFTGNVGASFVETKINLAAFAGQTILIRFRFASDP
                     SVGATGWYIDDILLESKPFVYTKAGLYDNTNTRVSYKDSLTPIIQALCADPPVVNAPT
                     VTQPTCGGTLTGTIVVNATGVSTLEYSINGGTTYQASNTFTGLAAGNYDIVVRLQATP
                     SCVTTYSSNPVVINALTGTPSITPPTVTQPLCPILTGTIVVNATGTGTLEYSVNGGTT
                     FQLSNTFSGLT"
     gene            4913..5980
                     /locus_tag="PLO70_14810"
     CDS             4913..5980
                     /locus_tag="PLO70_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75788.1"
                     /translation="MKKITIQTILFFLICFSFSAADGQMITGVWKGKINRQKVEVKII
                     QNGDSLTGTSYYYESANNYRRYSIRGYFDATTNEAVWWDDQLLEEKSSRFTISIPGKM
                     PLLSRADFNCPGGNKMMLDGKAAQKENDRVAKGEVHLDKNEQTDFEDEWDFVIDNYTV
                     GGNDPEIIDSINAIAQLPVKPAEELIGIKEETPKIIATEKPAQKETVPPEKINPVIEK
                     PQPVVKPAEPLKAPTIEEKFVTRQKVFIKEIPVSGDSIELRFYDNAEIDGDSISLFLN
                     DKLLFQHIKLTANAYAIKLAVQDLNERNELVMVAENMGAIPPNTSYMVAIVNGHRQDA
                     YLASTEGSSAMIQFVKAPPKN"
     gene            complement(6037..>6589)
                     /locus_tag="PLO70_14815"
     CDS             complement(6037..>6589)
                     /locus_tag="PLO70_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75789.1"
                     /translation="FIKQLHLHTRFIPNKLSMAFPSVGLINALRETATRLRNGAHYAW
                     GHHGACNCGNLLQVLTPLSEKEILSYAHTGAGEWTELAEEYCSSSNAPVNLVLSKLEQ
                     AGLTPTDIHHIEFLTDKAVLNFLPGGFRWLKRNVREDVILYFEAFANLLEEKLIQFVD
                     IRIEDIMATSLIGEKQVALESAV"
BASE COUNT         1925 a         1421 c         1401 g         1742 t
ORIGIN      
        1 ttaagaccag cagccggact ccaggaataa gaattagaat ttccggttgc agttaacgta
       61 acaggtccac actggttatt cggacctact gcaattgttg gaacaggatt aactgtaacc
      121 gttacacttg caggacccga actacatgta cccaatgtat tcgttacagt ataggtataa
      181 ttagtagtaa cagcaggtga ttgcaggaag taaacggttg aagcagctgt tccagccaca
      241 taaggtacag tagcagcagg atcagaatat aatccggttg caggagacca aatggcagtc
      301 ggtacttcaa aagtaatagt ccagctggtc aatgaaccag catcaccacc caaatcatca
      361 acaacgaaca aattccagtt gccgttgaag ttgcctgtaa agtttgacaa cggaggatta
      421 acctgggtaa ttgaacctgg gcctggtgca gggaatgcat ccggaccagc cgcattggtt
      481 ggcctgtacg ttcctgacac aattgtggca ggcaacaatg atgcagctgc atcatcaaac
      541 acaagattac catttacaat atcagttcca ccaccagcat cagacatgat caccacgttg
      601 gtaccggttg gcgattgaac caccatgtca atatctgacg ggaatgtatg tgaaagacca
      661 tttattgcaa ttgatttaac tcttacacca gaagttggta agccagaaat attaaccaca
      721 gccggataaa cactggcagg ggcacctgtt cctgcacctg ttcctgttcc gggtatggtg
      781 agagcagcac ctgaataagt gaccgtaaag gttgttggtg ccactgttaa tggctgaatt
      841 gttcctgcac aaattaccgg agctgcaggg gtaatagccg gtgtggccgg tgtacccaat
      901 acagttactg aagcagaatt ctggcaacca gttgcagtaa ttgttcctgt aactgtatac
      961 acagttgtaa cagttggcgt agcaattaca gttgtaccag ttgttgtatt taaaccacct
     1021 gaaggtgtcc aggcataagt atcagcatta ccactagcag ttagagttac cggtgcacac
     1081 aaaccacttg ggcctgagtt aacagttggt atcgggttaa cagtaagtga tgcagagttg
     1141 gaattggtag gaccacagat attctgaact acacaacgga acaaattacc gttatatact
     1201 aatcctgccg aagtaacagt aagtgtggtt gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nngttaatcc gctgaaagta ttagataatt ggaaagtagt gccgccattt
     1381 acactgtatt ccaatgtacc ggtacccgtt gcattcacaa caattgttcc ggttaatatt
     1441 ggacagagcg gttgagttac tgttggcgga gtaatggatg gagtaccagt cagtgcattt
     1501 atcactacag ggttactgct gtaggtggtc acacacgaag gtgtggcctg caaacgaact
     1561 acgatatcat agttacctgc tgcaagacct gtaaatgtat ttgaagcctg gtatgtcgta
     1621 ccaccattaa tactatattc taatgtacta acgccagttg catttacaac aattgttcct
     1681 gttaaggttc ctccacaggt tggctgtgtt acggttggag cattaaccac aggcgggtct
     1741 gcacaaagtg cctgaatgat cggggtaaga ctgtctttat aacttaccct ggtatttgta
     1801 ttatcataca aacctgcttt ggtataaaca aaaggcttgc tctctagcaa aatatcatca
     1861 atataccatc ctgttgcacc cactgaagga tcagatgcaa acctgaatct tatcaatata
     1921 gtttgaccgg caaatgctgc caggttaatt tttgtttcaa caaatgaagc ccctacgttt
     1981 cctgtaaatg cctggcggcc tcccaggaat gtattcaatg ctgataatgt accattatac
     2041 cgggctcctg acatgtatgg tccaaggtca gtccatgtgc caccactgtt agtgctgatc
     2101 tcaaccacgc aaccatcaaa tccgctttct gtaacatacc tgtgccagaa actaagcgaa
     2161 gtgatggctg aaggattagc agtcagcaca attggatttg tcgtcgttaa aatctgttga
     2221 gacgacgcag ctgggttagg tgaaaagaaa gccagcggtg cacttcttac cgaagcaccc
     2281 gatgtagccc atacactagc tgaagtggaa gtcgcagtcc agttaggtgc aatggcggta
     2341 accggatcat tgatatgctc taccggtgca aaataggtac ctgcattgac attaacagtc
     2401 accggaaaat tctgagaagt gttaatggca aggttgatcg agctgaatgt tactaccctg
     2461 tctcccgggg tatacacacc accgctaacc caggttacag tagttggcaa tgtatccctc
     2521 aatacatagt ttgccatcgg tgtgcattcg cctgcactta caacgtttgt atatgtaaga
     2581 ggagttcctt caaacactga aggaccgctt ggattaaggc caaaatttcc ggaatcaaca
     2641 gagaaatcag gtacaccatc tgagatgctg gtgttagaac cttgagatgc attacgtccc
     2701 aatcctcttt tagcaaaagc tttccagata gagcaggcat aacgaccacc aaagaaaacc
     2761 tggtcagctt gtaagattgc attcctggat tgtacaaaac ccggactaca aggctgcaac
     2821 ctcattcctt ccataaccag tttcatagct gctgcattgc ctctccatgt tgaaaccgca
     2881 cccggaggac tcataaagtt tggttcaatg aaattatcct gtttgataat ctcccaggtc
     2941 atttcccata gcataccaca ccatatagta ccaacaccat gtggtactgc ctgacccggc
     3001 agattagcat aggtcaggga gaagatagac atatctgtag aatatggctg agcccggata
     3061 ccatttccgg ttataggttg atttaaagcg taagtgccaa tacctctcct gatcggtccg
     3121 tcatttatag ttgcagtagc ccagttcgtt cccatcatca aagacatata atcactccat
     3181 ccttcaccac cctgttcggc attttgcaga cagttaacca cgtttggtcc accagtaaac
     3241 ctgttactga taccatgtcc atattcatga cttacaattc cattatctaa gtcaccatcc
     3301 ctgtcaggat tgggtgctgt ccataagaac atttgcattc ttggcctgga gccatcagct
     3361 ggtgtggcaa agttggcatt gttcgtacca ctggcatcct gagcatcggc aattacatag
     3421 tcaacaccag caccaccgcg gccctggttg tcattctgaa aattacctgc tacttcatca
     3481 aacccgtacg tatatgccat atcatgcatc aggttattca tgtaaaacaa gtttacaata
     3541 gcagcctgct ggttgtcaga agtggggtca ataggactct ttgtaaaatc gtaggtataa
     3601 tcaaaagtca gattcggtaa tggggtagtt gagttacccg taataccgaa agttgtatta
     3661 ttagcatccc tatcttcctg ggcccatacg ttattaccac gggtgatatt ataatcagcc
     3721 gggtcactgt gccattttaa agaagttgca ttaccgggcg tcaatgtcca cggatctgta
     3781 tgcaatgaag gtgtacctcc ggggtgctgg gggctttctg caggatattt aaccacacga
     3841 taagtagcag tcccaattac aaatggtttg tactcccatt tattactttt atcattacgc
     3901 tgcatcacat agttcatgga actggttgca gattttttat tatgtttttc tgcaacatgt
     3961 ttctctattg aatggttctc atgatcccat ttacaggtaa tagtcaggtt atctttattt
     4021 aatatagtcc ctgtttgagc atctactctg atcagccagt agtctgatgt gctaaccgga
     4081 gccacgaaca cctgccaggc taagcgatat tcgccccttg cattaggcat ccagatcaat
     4141 tcggcactta tgttctcggc agaaacgccc aacttaccaa attcaaattt gcggccatct
     4201 tccagtacgc tgataggcac tgccagttca gtggcttcta cttttgcttc tctgaaagct
     4261 gactgtaaag catttgccgg agaaatacca ggcaaattgc ttgcattacc agtaagcttc
     4321 tccatagatg gttgcagttc tcctgcatcg gataccacct ttccacccct gaaagccagt
     4381 gtttttaact ggttataaac agggatgccc ttgtagctct gctgcaggta caccattatc
     4441 acatcagtcc cggtaatctg gtaactgctt gatacgataa catcattaat gtcttgagag
     4501 ggcaatttaa tagcagcagc gttttgcctg acaagttgta gcgctgcatt tttggttttg
     4561 ttatcaacct gtgcaaagct ggtgaaagca caaatcaata aaccgataaa aagtagaact
     4621 tttctcataa aagttagtgt tgggttttag atgccgaatt acttccagtg aaattcaaca
     4681 ggtacgatgg ttatttatga ggatatgaga ttcgggacat gcaaataccc gaaataagag
     4741 gaagaatagt aaatactttc ctcataagca ggtaatggca caaaatacgg gactaatata
     4801 ggaaataaaa caaattccag ccaaaacaaa tccattccat ttccgggtaa aataatagtt
     4861 gcccgtgatt cgtttaactg aataaccctg aaaactcatt taaacctgca ttttgaaaaa
     4921 aataacgata caaacaatac tttttttcct tatttgtttc tctttctctg ctgcagatgg
     4981 ccaaatgatc acaggagtat ggaaaggaaa gataaaccgg caaaaagtag aggtaaagat
     5041 cattcaaaac ggagatagtc ttacaggtac ttcgtattat tacgaatcag ccaataatta
     5101 ccgccgctat agtatcaggg gatattttga tgccaccacc aacgaagccg tatggtggga
     5161 tgaccagtta ctggaggaaa aatcaagccg gttcaccatt tcaattccgg gaaaaatgcc
     5221 cttactgtca agagcagatt tcaattgccc cggaggtaac aaaatgatgc tggatggcaa
     5281 agctgcacaa aaagaaaatg accgggttgc aaaaggggaa gttcatttag ataaaaatga
     5341 acagacggat tttgaagatg aatgggattt tgtgattgat aattataccg tgggcggcaa
     5401 tgacccggaa ataattgata gtatcaatgc gatagcacag ctaccggtaa agccagctga
     5461 agaacttatt ggaataaagg aagaaactcc caaaataatt gcaactgaaa aaccggcaca
     5521 aaaggaaaca gtgcctccgg aaaagatcaa tcccgtcatt gaaaaacctc aaccggttgt
     5581 aaaacctgcc gaaccgctga aagccccgac aatagaagaa aagtttgtta cccgccagaa
     5641 agtatttatt aaagaaatac cggtaagcgg ggactccata gaacttcgtt tttacgataa
     5701 tgccgaaatt gatggcgatt ccatctctct tttcttaaat gataaactgc tgtttcagca
     5761 tataaaactg acggcgaatg cttacgcaat caaactggca gtacaggatc tgaatgagcg
     5821 caatgaactg gtgatggtag ccgaaaatat gggagcaatc ccacctaata cttcttatat
     5881 ggtggccatt gtaaacgggc accggcagga tgcctacctg gccagtaccg aaggaagcag
     5941 tgcgatgata cagtttgtta aagcacctcc aaagaattaa taaaaaaacc ggtgagttct
     6001 tcaccggttg tcaggaaaag aaagttacca cgctgcctat actgcacttt ccaatgccac
     6061 ttgcttttcc ccgattagag aagttgccat aatatcttct atccggatat caacgaattg
     6121 aattaatttt tcttccagca ggttggcaaa agcttcaaaa taaaggatca catcttcccg
     6181 tacatttctt ttcagccagc gaaaaccgcc gggtaaaaaa ttaagtacag ccttgtccgt
     6241 aaggaactct atatggtgaa tatcggtggg tgtaaggccg gcctgttcca gtttcgataa
     6301 taccaggtta acgggtgcat tactcgaaga gcaatattct tcagccagtt ctgtccattc
     6361 gccggcacca gtatgggcat aactcaggat ttccttttca gacaagggtg ttaatacttg
     6421 cagaaggtta ccacaattac aggctccatg atgcccccag gcataatggg caccattgcg
     6481 taagcgggtt gctgtttcac gcaatgcatt gatcaaaccg acagagggaa atgccataga
     6541 caatttgtta ggtataaaac gggtatgaag atgtaattgt ttgataaaa
//
LOCUS       DAORTI010000164         6488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_13874_length_6488_cov_9.389243, whole genome shotgun sequence.
ACCESSION   DAORTI010000164 DAORTI010000000
VERSION     DAORTI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13874_length_6488_cov_9.389243"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..379
                     /locus_tag="PLO70_14820"
     CDS             98..379
                     /locus_tag="PLO70_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75790.1"
                     /translation="MKRRVRKIANFFAFNLLFFALYLNFIHKDKAVVGSAQAIPSTTA
                     AFSETVLVDNPEQYLQKKNETAAVSVDKEQKNASKSTAENSTALKLSIN"
     gene            473..3328
                     /gene="polA"
                     /locus_tag="PLO70_14825"
     CDS             473..3328
                     /gene="polA"
                     /locus_tag="PLO70_14825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQZ75791.1"
                     /translation="MEKRVFLLDAFALVFRAYYALIRNPRLTSKGKNTNAQFGFTNAL
                     VELINKQKPSHMAVCFDTSAPTERHTDFADYKANRQETPEDILAAVPDIKKIIEGFNI
                     PVIYADGFEADDVIGTLSKQAEKAGYEVYMVTPDKDYGQLVSEKIKIYKPAYQGNDAE
                     ILGPEEVCAKWNIKDVSQVVDMLGMMGDAVDNIPGIPGVGEKTAAKFLAEYGSLENTL
                     ANADKIKGAMGEKVKKGKEMAILSKKLATIITTVPVEFHEEDFKLKDWDKEKLKEVFA
                     ELEFRTLGKRLLGDEFSVAAPGKSVIEKEIPQGVQTDLFGNIVESPGSVVSSQVPIQD
                     PELASEILSVDKNINNTPHKYEAVEGETAIKQLVAALKKHDEICFDTETTGIDANDAE
                     LVGLSFSVNPGEAYYVPCPADQKKTKEILTHFDTLFSDKKKLWIGQNTKYDLLVLKWY
                     GIEVAGNIFDTMLAHYVIEPDGKRSMDILSEKFLGYEPVHIEELIGKKGKMQGNMRDV
                     EIEKIKEYAGEDADITLQLKNVFTPLLKQKEVEKVFNEVENPLVKVLVDMEFEGIKVD
                     VDFLSDYSKELEKDAKKAEESVYKQAGVRFNLASPKQLGEVLFDKLKLDPSAKKTKTG
                     QYQTGEDVLLKLAVKGHQIVDDILTFRELTKLKSTYVDSLPQLINKKTGRVHTTYGQA
                     VAVTGRLASNNPNLQNIPVRTDRGKEIRKAFIPRDSKHILLSADYSQIELRIVASISG
                     DANMCKAFKDGTDIHTATAARVYNIDEKDVTKEMRYKAKSVNFGIIYGQGAFGLADNL
                     GISRAEAKEIIDNYKKEFPGIQKYMDDTINFAREHGYVQTLMGRKRWLRDINSANFTV
                     RGFAERNAINSPIQGTAADMIKLAMQKVHTALKKEKMESRMILQVHDELVFDAVKSEV
                     KELKPLIVECMQSAMLLPHNVPVIAECGEGNNWLEAH"
     gene            3382..4041
                     /locus_tag="PLO70_14830"
     CDS             3382..4041
                     /locus_tag="PLO70_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQZ75792.1"
                     /translation="MQPVVYYCYDAYCGWCYGFSPVIKKIATAFKDKLSFEVLSGGMI
                     PKENARPISAIAGYISEAYHRVEETTGIKFGEDYLWHIFNPDKSDWYQHSEKSASALC
                     IVKEIQPEQQVAFAADLQYALHYEGRDLTDDEAYRHLLEKYVIPEEEFYTKLNSEEYK
                     EKAHYEFALCKQLQATSFPQVLLQVADQKFYLLAKGYTDYETLKKRIQTVLAETQFTS
                     A"
     gene            4059..4976
                     /locus_tag="PLO70_14835"
     CDS             4059..4976
                     /locus_tag="PLO70_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HQZ75793.1"
                     /translation="MILTITLNPCIDKSSRVEKLKPESKLRCAEVVNEPGGGGINVSK
                     ALKKLEAPSVALFPAGGHNGNMLCSLLKEEEILFHAVDTKVETRENWIVLETSTNNQF
                     RFTFPGREVMEETIKSLVDQIRSFAPTYVVASGSLPPGLPDYFYGLIVKNATAVGAKC
                     IVDTSGPALQALKGKNAYLIKPNIGELCKMLNVDWLDKEEVPDAAQQAIRDGFAEVIV
                     VSMGHLGAWMVSEDKRYFVDAPPVEKKSTVGAGDSMVAGITYSLQKGKTLREAIRFGV
                     ACGSAATMNDGTQLFNKTDAEKLFAIINE"
     gene            4969..5346
                     /locus_tag="PLO70_14840"
     CDS             4969..5346
                     /locus_tag="PLO70_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQZ75794.1"
                     /translation="MNRDIENREDLLLLVTRFYEKLLADDSINYIFTEVAKINLEHHL
                     PVLVDFWDSILFQSDTYRKNAMQPHLILHQQSPLQKHHFETWLRYFKESVDEYFDGTK
                     AFLAKERATSIATVMQIKISQLQ"
     gene            5441..6100
                     /locus_tag="PLO70_14845"
     CDS             5441..6100
                     /locus_tag="PLO70_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQZ75795.1"
                     /translation="MKKILLTCLSVSVILFACNNEKKTDDSKKKETETTEINNTSTAP
                     PDSAAQAEMMKAWQDFATPGPEHTWMNNHAGTWVCDSVAQWMDPAQPPTYSKATDVVT
                     VAMNGLYQMSDFSSNMMGMEMKGHGMMGFDKMKKKFVLSWIDNLGSGIVQMEGSYDEA
                     TKTLTMSGKQSDPGMKKETAMRQVLTFHNDDSYTMAMYGTGHDGKEAKFMEGTFKRTK
                     K"
     gene            complement(6153..>6488)
                     /locus_tag="PLO70_14850"
     CDS             complement(6153..>6488)
                     /locus_tag="PLO70_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HQZ75796.1"
                     /translation="ISGYHLLMILTNVDGKLNVNEDLVIRDWLSQHFPVRADLDKEME
                     VISTLERSDYPSHFHTHMNLFYQHSEKQDRLQLLQFAMNIIKADGEISNEENSFFNEL
                     YDAWNEEAE"
BASE COUNT         2161 a         1206 c         1413 g         1708 t
ORIGIN      
        1 aaaacattcc ctctttactt gcagctggta gtacatgcgt ttatcttagc cgcagatatc
       61 cgataaacca ttttttaaaa tccaataaat ccgaatcatg aaaagacgtg tccgtaaaat
      121 cgccaatttt tttgcattca acctgttgtt cttcgccctc tacctgaact ttattcataa
      181 agacaaagct gttgttggca gtgcccaggc aataccatca actactgccg cttttagcga
      241 aaccgttctg gtagacaatc ctgaacagta cctgcaaaag aaaaatgaga ctgctgctgt
      301 ttctgttgat aaagagcaaa aaaatgcttc taaatcaaca gcagagaatt caacagccct
      361 gaaactttct attaattaag caaagcagcg ttaacatatc agcagcccgg ccttttagcc
      421 gggcttgttg tgtttttaga tgcgacacgg gtgaaatctt taccttagag gcatggaaaa
      481 aagagtcttc ctccttgatg cctttgccct tgtattccgt gcctattatg cacttatccg
      541 taatccccgc ttaacctcca aaggcaaaaa taccaatgcg cagtttggct ttaccaatgc
      601 gttggttgaa ctgatcaata aacaaaagcc atcgcatatg gctgtatgtt ttgatacttc
      661 cgctcccaca gaacgccata ctgattttgc agattataaa gctaaccggc aggaaacacc
      721 agaagatatt ttagctgcgg tacccgatat taaaaagatc atagaaggtt ttaatatccc
      781 ggttatatat gctgatggtt ttgaagctga tgacgttatt ggcaccttaa gtaaacaggc
      841 agaaaaagca ggatacgaag tatatatggt aacaccggat aaagactatg gtcagctggt
      901 ttctgaaaaa ataaaaatat ataaaccggc ttaccagggt aatgatgcag aaatactcgg
      961 tccggaggaa gtctgtgcca aatggaatat aaaagatgta agccaggtag tagatatgct
     1021 gggtatgatg ggtgatgccg tagataatat acccggtatt cccggtgtgg gagaaaaaac
     1081 agccgctaag ttccttgctg aatacggatc actggagaat acattagcta atgcagataa
     1141 gataaaaggg gccatgggag aaaaagtaaa gaaaggaaaa gagatggcca ttctttctaa
     1201 aaaacttgct actatcatta cgactgtccc tgttgagttt catgaagaag attttaagct
     1261 gaaagactgg gataaagaaa aactgaaaga agtatttgca gaacttgagt tcagaacatt
     1321 aggcaaaaga ttattgggtg atgaattctc cgtggcagca ccgggaaaat ctgttataga
     1381 gaaagaaata ccacagggtg tacaaacaga cttgtttgga aacattgttg aaagtcccgg
     1441 ttccgttgtc agtagccagg taccaataca agacccggaa ctagcatcgg aaatattaag
     1501 tgttgataag aacattaata acactcctca taaatatgaa gcggttgaag gagagactgc
     1561 aatcaagcaa ctggtcgcag cattaaagaa acatgatgag atttgttttg atactgaaac
     1621 caccggcatt gatgccaatg atgcagagtt ggtaggactt agtttttctg taaacccggg
     1681 agaagcttat tatgttccct gtccggctga tcaaaagaag acaaaggaaa tattaacaca
     1741 ctttgataca ttgttcagtg ataagaagaa gctatggata ggccagaaca ccaagtatga
     1801 cctgctggta ttaaaatggt acggaatcga agtagccggc aatatcttcg acaccatgct
     1861 ggcccattat gtgattgaac ccgatggaaa aagaagcatg gatatactca gtgaaaaatt
     1921 tctgggctat gaacccgtgc atatagaaga actgattggg aaaaaaggaa agatgcaggg
     1981 taatatgcgg gatgtagaga tcgaaaagat aaaagaatat gccggcgagg atgcggatat
     2041 cacattacaa ctgaaaaatg tattcactcc gttattaaag caaaaagaag ttgagaaagt
     2101 ctttaatgaa gtagaaaacc ctttagtaaa agtattagtg gatatggaat ttgaaggaat
     2161 aaaggttgat gtagattttc ttagtgatta ttccaaagaa ctggaaaaag atgcgaaaaa
     2221 ggctgaagaa agtgtgtaca aacaggcagg tgtacgcttt aatctcgcat ctcccaagca
     2281 gttaggcgaa gtattatttg ataaactgaa actggatccc tctgctaaaa aaacaaagac
     2341 tggtcagtac cagaccgggg aggatgtgtt gctgaaattg gctgtaaaag gacaccagat
     2401 agtagatgat attctaactt ttcgggaatt aacaaagttg aagtcaacct atgttgattc
     2461 acttccgcaa ctgatcaata aaaaaacagg gcgggtacat accacctatg gacaggctgt
     2521 ggctgtgaca ggaagattag ccagtaataa tcccaacctg caaaatatac cggtcagaac
     2581 agatcgggga aaggagatac ggaaagcttt tattccaaga gacagtaaac acattttatt
     2641 atccgccgat tactcccaga ttgaattaag aattgttgca tccatcagtg gtgatgccaa
     2701 tatgtgtaaa gcatttaaag acggaacaga cattcataca gccacagcag cccgggttta
     2761 taatatcgat gagaaagacg ttaccaaaga aatgcgctac aaagccaaaa gtgtaaactt
     2821 cggaatcatt tatggacaag gagcatttgg attagccgat aacctgggca tctcaagagc
     2881 agaagctaaa gagatcatcg acaactacaa gaaagaattt ccaggtattc aaaaatacat
     2941 ggatgacact atcaactttg ccagggaaca tggttatgtg cagacattaa tgggaagaaa
     3001 aagatggcta agggatatta attccgccaa cttcactgtc cgtggttttg cagaaagaaa
     3061 tgcgatcaac tcccccattc agggcactgc tgccgatatg atcaaactgg ctatgcaaaa
     3121 agtgcatacc gctttaaaga aagaaaaaat ggagagtaga atgatcttac aggtgcatga
     3181 tgaattggtc tttgatgcgg tgaaatcgga agttaaagaa ctaaagcctt taatcgtaga
     3241 atgtatgcaa tcagccatgc tgctgccaca taatgtaccg gtgattgcgg aatgcggtga
     3301 aggtaataac tggctggaag cgcattgatt tttctaaagt cataaacttt gacttcttta
     3361 tatgcataag tttgcactct tatgcagcct gtagtttatt attgttatga tgcttactgt
     3421 ggctggtgct atggtttcag ccctgtgatt aaaaaaatag caacagcctt caaagacaag
     3481 ctttcatttg aagttttatc aggaggaatg atcccaaagg aaaatgcccg gcccatttcg
     3541 gcaattgcag gctatatcag tgaagcctat caccgggtgg aagaaacaac aggaattaaa
     3601 tttggcgaag attacctgtg gcatatattc aatcctgata aaagtgactg gtaccagcat
     3661 tcagaaaaat ctgcctcagc tttatgtatc gttaaagaaa tacagcctga acagcaggta
     3721 gcctttgctg cagatttaca atatgccctg cattatgaag gaagagacct gacagacgat
     3781 gaggcctaca gacatttact ggaaaaatat gttataccgg aagaagagtt ttatactaaa
     3841 ctgaatagtg aagaatataa agaaaaggca cactatgaat ttgccttgtg taaacaactg
     3901 caggcaacaa gcttcccgca ggtactgctg caggtggcag atcagaaatt ctatttgctt
     3961 gcaaaaggtt ataccgatta tgaaacattg aaaaaaagaa ttcaaacagt gctggcagaa
     4021 acacaattca cttccgctta actcaattat tattttgtat gattttgacc attacactta
     4081 atccctgtat tgataaaagc tcaagagtag aaaaattaaa accggagagt aaattacgct
     4141 gtgcagaagt ggtaaatgaa cccggcggtg gtggtatcaa tgtcagcaag gcattaaaaa
     4201 aactggaagc tccctccgtc gcattattcc ctgcgggcgg acataatggg aacatgttat
     4261 gttcattact gaaagaagaa gaaattttat ttcatgcagt ggataccaaa gtggaaacca
     4321 gggaaaactg gatagtgctg gaaacatcca ccaataatca attccgtttc actttcccgg
     4381 gtagagaagt tatggaagaa acgataaaat ctttggttga ccagatccgt tcttttgcac
     4441 caacttatgt ggtagcaagt ggcagtttgc ctcccggctt gccagattat ttctacggtt
     4501 tgatcgtaaa aaatgcaacg gcagttggtg ccaaatgtat tgttgataca agtgggccgg
     4561 cattacaggc attaaaagga aaaaatgctt acctgatcaa gcctaatatt ggtgaattat
     4621 gtaaaatgct gaatgtggat tggctggata aagaagaggt acccgatgca gcacagcaag
     4681 ccatcaggga tggttttgcc gaagtaattg ttgtttcgat gggacacctg ggtgcctgga
     4741 tggtaagcga ggataaaaga tattttgttg atgcgccacc cgttgaaaag aaaagtactg
     4801 ttggcgcagg agatagtatg gtggcaggta taacatactc gttacaaaag ggaaagacac
     4861 tcagagaagc tattcgtttt ggtgtagcct gcggcagtgc agccaccatg aatgatggca
     4921 cacaattatt caataaaact gatgcagaaa aactctttgc cattatcaat gaatagagat
     4981 attgaaaaca gggaagatct attgttactc gtcaccaggt tctatgaaaa attacttgct
     5041 gatgattcca tcaattatat atttactgaa gtggccaaaa taaatctgga gcatcatttg
     5101 ccggttctgg ttgacttttg ggattcaata ttatttcaat ccgatacata ccggaaaaat
     5161 gcgatgcagc cacatcttat attacaccag caatctccgt tgcaaaaaca tcattttgag
     5221 acctggctac ggtacttcaa agaatcggta gatgaatatt ttgacggcac aaaagcgttt
     5281 ttagccaagg aaagagcgac cagcatcgct accgttatgc agatcaaaat cagccagtta
     5341 cagtaattac agaacgttca tccacttata gtttatttct tccgtttctt ccttgcaaaa
     5401 tgtcaactga attagtacct tattcaccta aaactctttt atgaaaaaaa tactattgac
     5461 ctgcttatct gtatcagtca tcctgtttgc ctgtaacaat gaaaagaaaa cagatgacag
     5521 taaaaagaag gaaaccgaaa caaccgaaat caacaacact tcaacagccc ctccggattc
     5581 tgcagcacaa gctgaaatga tgaaggcctg gcaggatttt gctacacctg gacctgagca
     5641 tacctggatg aacaatcatg ccggaacatg ggtttgcgat tcagtagcac agtggatgga
     5701 tcctgcacag ccgcccactt attcaaaggc aaccgatgtg gttacagtag ccatgaacgg
     5761 actgtaccag atgtctgatt tttccagcaa catgatgggc atggaaatga aaggccatgg
     5821 catgatgggt tttgataaaa tgaaaaagaa atttgtactt tcctggatcg acaacttggg
     5881 ctccggcata gtacaaatgg aaggaagtta tgatgaagca acaaagactc ttactatgtc
     5941 aggtaaacaa tctgatcccg gcatgaaaaa agaaacagcc atgcgacagg tattgacatt
     6001 tcataatgat gatagttata ctatggctat gtatggtaca ggacatgatg gcaaagaagc
     6061 taagtttatg gaaggaactt ttaagagaac caaaaaataa cgaaagctgt tttccacaaa
     6121 aaacccggaa ctatttccgg gttttttcat tgctattccg cctcctcatt ccaggcgtca
     6181 tatagttcat taaaaaagga attctcttca ttggagattt ccccatctgc cttaataata
     6241 ttcatggcaa actgaagcag ttgtaaccgg tcttgttttt cagagtgctg gtaaaacagg
     6301 ttcatatggg tgtgaaaatg tgaaggatag tcacttcttt ctaatgtgct gatcacttcc
     6361 atctccttgt ccaggtctgc ccttactgga aagtgctggc tgagccagtc cctgatgacc
     6421 agatcttcat ttacattcaa ttttccatct acattggtaa gtatcatcaa aaggtgatac
     6481 ccggatat
//
LOCUS       DAORTI010000165         6368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14301_length_6368_cov_7.451925, whole genome shotgun sequence.
ACCESSION   DAORTI010000165 DAORTI010000000
VERSION     DAORTI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14301_length_6368_cov_7.451925"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLO70_14855"
     CDS             complement(<1..389)
                     /locus_tag="PLO70_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQZ75797.1"
                     /translation="MADIHSILKRYWGFDSFRPLQEDIINAVLKGRDTLALMPTGGGK
                     SLCYQVPAMAREGLCLVISPLIALMKDQVENLRRKGITAFAIYSGMSRKEVINTFKVA
                     SDSNCKFLYVSPERLETSLFKEYLPGLG"
     gene            complement(404..2515)
                     /locus_tag="PLO70_14860"
     CDS             complement(404..2515)
                     /locus_tag="PLO70_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQZ75798.1"
                     /translation="MEQVQWKVDGMDCTTCALNIQKYLEKKGMKNIKVNFATGDVSFY
                     VKGQFNEKEIAKGINGLGYEVAEHKPESLKRKPFLTTHLQRFWFCFPFTAVLMLHMIP
                     GIHIHWLMNHWVQLALTIPVYLVGMGFFGKSAWKSIRNGMPNMNVLITIGSTAAFVYS
                     LYGSLTGQAAQYMFYETAATIITLVFLGNFLEDASIASTQRELNKLVKSQKVMANMIA
                     FDDEHKEHVFPVENIQLRVGDLILIKSGEQVPVDCKVLWGDVHVNEAIITGESLPVHK
                     YAKDKLIGGSIIHDGTVKAQVTAVGDDTVLSGIINMVKQAQGEKPPVQLLADKISAVF
                     IPVVLVIAAVTLIASWIVLKDFTPALMRSIAVLVIACPCAMGLATPAAIAVGLGRAAK
                     TGILFRNATSLETFKDIKQVVFDKTGTLTTGKFIVDGFRALNGDEEQFKKTVYSLEKY
                     SNHPIAKCITDAWKTKDQIQWKKIEEVKGLGMQAEDKEGNNYTAGSYKCAEGLTKDDT
                     HNVYILRNGLLLGWIDVKDEIRPEAKSIIDYLHNKDIKTILLSGDRLATCTQLAVKLG
                     IDTVFAEQTPQQKLNKVAELSSVVPTAMVGDGINDAPALAKATIGISMNEASQIAVQS
                     AQVVLMNHGLKNLPTALGLGKHTYLTIKQNLFWAFAYNIIAIPVAAFGFLTPTFGALV
                     MGLSDVVLAINSVRLFVKKVV"
     gene            2606..3031
                     /gene="tsaE"
                     /locus_tag="PLO70_14865"
     CDS             2606..3031
                     /gene="tsaE"
                     /locus_tag="PLO70_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQZ75799.1"
                     /translation="MEWKFSLDSIKEIATAFWEAAGNASVIAFHGQMGTGKTTLIHAL
                     CDVKGVKDVVSSPTFSIINEYVYDCEGTKKPLFHMDLYRLKDEQEAQQAGVEDALHSG
                     HICLVEWPEKAPGIFPDNTFHAYLELIDSKTRRLKISNN"
     gene            3075..4289
                     /locus_tag="PLO70_14870"
     CDS             3075..4289
                     /locus_tag="PLO70_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQZ75800.1"
                     /translation="MSQQRPKISTSFSYETLEETLDIKPRAEGMMIGIPKEIAFQENR
                     IALTPDAVSVLISNGHQVMIEHNAGEASHFRDKDYSEAGAKIVYDRESVFKAPILVKS
                     APIIDEDLPLLQLNQIIISPIHLAVMKAEILEKMMEKRVTAISFENLKDDSDSYPIVR
                     SMSEIAGSAVMLIAAQYLGSANHGKGVLLGGVSGIAPTKVIIIGAGIVGEYAARAALA
                     LGASVKVFDNSVYRLKRLQNNIGQRMWTSVIEPRLLAKQLKTCEVAVGALGSETGRTP
                     LIVTEEMVSNMRPGSVIIDVSIDRGGCFETSEITSHEHPIFMKYGVIHYCVPNIPSGF
                     ARTASQAISNVLMPLLLEAGEEGGFENLVWHKLHLRSGIYLFKGALTNFYLSQRFDLK
                     YTDLNLLIASQR"
     gene            4396..5199
                     /locus_tag="PLO70_14875"
     CDS             4396..5199
                     /locus_tag="PLO70_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75801.1"
                     /translation="MRNVLIIMAGLFVLACNNQEDKKAEKKVKYSDLVNERLNGNVQS
                     VEETPYQVDSAGKIGNMDSCCIDLVEYDENGNAVKFTSKDSKGTVKNQSVYTRHETGL
                     WIGSTDTKEGGKPSGSMKVGVDDKGQYTVAEAFDSTGKLDVYYTDTKQNEFGQILGWK
                     QYDKDSVFRMQGEATYDKGLQMGFTTKDSVGKLKSSSSFKYNDKGEQTEALQTNVIKD
                     STTTKVTKYTYDSHDEMGNWTQRTTFDDKGKAIKIVKRVYTYRKDEEKK"
     gene            complement(5264..>6368)
                     /locus_tag="PLO70_14880"
     CDS             complement(5264..>6368)
                     /locus_tag="PLO70_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75802.1"
                     /translation="DMPGWSKTYGAYSEAELKVNQKQKLLLRADGSSTFLKASMTMYQ
                     PGELPMYMLTWPDNRKNQYGISASWLMQIDSTVKLQVTGRTDFITHNLVSQEAKDQVS
                     IFGYPSANRNDLLKNLSVLFSKKLSQKIKVSASIAYTERMPTASELYGFYLFNSNDGY
                     DYIGNPKLNIEKSLQADLSAIYNWKNYRVQLSYFYSSLFDYITGTINSSFSTMTIGAN
                     GVKEFINIPDASVSGMEGSLILKPSPAIDVVSTIRYTAGKDNKGNPLPFIAPLKNTTS
                     VRYQPKNFSVQLEAEAASKQDCINTEAGEDITTGYSLLHIRFGYNTMIFNNIIELQGG
                     VENIFDRKYHEHLDWGNIPRPGRNLYIQFRIHL"
BASE COUNT         1877 a         1268 c         1347 g         1876 t
ORIGIN      
        1 ccaagtccgg ggaggtattc tttaaataag gaagtttcca gccgttccgg agaaacatat
       61 aaaaatttgc aattgctatc ggaagctact ttgaatgtat tgataacttc tttacggctc
      121 atcccggagt aaatagcgaa agcagtaatc cctttacgac gaagattttc cacctggtct
      181 ttcatcaacg caatcaatgg agagatgaca aggcataaac cttcccttgc cattgcaggt
      241 acctggtaac aaagagattt accaccaccg gtcggcatca gggctaatgt atctcttcct
      301 ttcagaaccg cattgataat atcctcttgc aggggacgaa agctatcgaa gccccagtac
      361 cgttttaata tggagtggat atccgccatc gggaagtgtg aatttacact actttcttaa
      421 cgaagagacg tactgaatta atagccagca ccacatcact caatcccatc accaatgcac
      481 cgaatgtggg agtaagaaaa ccaaaagctg ctacgggaat agcaataata ttgtaagcaa
      541 aagcccagaa gagattttgc tttatcgtta aataggtgtg tttgcctaac cctagggcag
      601 taggaagatt ttttaaacca tgattcatga gtacaacctg cgcactttgt acggcgatct
      661 gcgatgcctc gttcatggaa ataccgatcg ttgctttggc taatgccggg gcgtcattaa
      721 tgccatcacc caccatggct gttggtacaa cggaactcag ttccgctact ttatttaatt
      781 tttgctgtgg cgtttgttca gcaaatacag tatcaattcc aagtttcact gccagctgag
      841 tacatgtagc cagcctgtct ccgcttaaca aaattgtttt tatatcctta ttatggagat
      901 aatcaatgat actctttgct tctggcctta tttcatcctt tacatcaatc caacccagta
      961 acaaaccatt gcgtaggata tagacgttat gtgtatcatc ttttgttaat ccttcagcac
     1021 atttataaga accagctgtg tagttattcc cttctttatc ttcagcctgc atgcccaggc
     1081 cctttacttc ttcaattttc ttccattgaa tctggtcttt tgtcttccag gcgtctgtga
     1141 tgcacttagc aatcgggtgg ttggaatatt tctcaagtga ataaacggtc tttttaaatt
     1201 gttcttcatc accattcaat gcccggaagc catccacaat gaattttccg gtagtcagtg
     1261 ttcctgtttt atcaaatacc acctgtttga tgtctttgaa tgtttccagg ctggtagcgt
     1321 tgcggaaaag aattccggtt tttgcagctc ttccgagtcc tacggcaata gcagccggag
     1381 tagctaaccc catcgcacag gggcaggcaa tcactaatac agcaatactt ctcatcagtg
     1441 caggagtgaa gtcttttaga acgatccagc tagcaatcag tgtaacagct gcaataacca
     1501 gcacaacggg gataaataca gcactgattt tatctgccag cagctggaca ggaggttttt
     1561 ctccctgtgc ctgtttcacc atatttatta tgcctgataa aacagtatca tctccaactg
     1621 ccgttacctg tgctttcacg gttccatcat gtatgatact gccgccaatg agtttatctt
     1681 ttgcatattt atgaacaggt aaactctcac cggtaattat ggcttcattt acatgtacat
     1741 caccccaaag tactttgcag tcaaccggga cctgttctcc gctttttata agaattaaat
     1801 cccccactct taattgtata ttttcaaccg ggaacacatg ttctttatgt tcgtcatcaa
     1861 aagcaatcat attggccatc actttttgtg atttcaccaa tttattaagt tctctttgag
     1921 tagatgcgat agaagcatct tctaaaaaat tgccaagaaa gaccaaagtg atgatggtgg
     1981 ctgcagtttc atagaacata tattgtgccg cctgccctgt taatgaaccg tataagctgt
     2041 aaacaaatgc tgctgttgaa cctatcgtta tcaggacatt catatttggc ataccgttgc
     2101 ggatactttt ccatgcactt ttgccaaaga aacccatacc gactaaataa acgggaatag
     2161 taagggccag ctgcacccag tgattcatca gccagtgaat atggataccc ggaatcatat
     2221 gcagcattaa tacagctgta aaaggaaaac agaaccagaa tcgttgcagg tgagtggtga
     2281 gaaaaggttt tctttttaag ctctcaggtt tgtgttcagc cacttcgtaa cccagcccgt
     2341 taatgccttt agcgatctcc ttttcattaa actggccttt tacatagaaa cttacatcac
     2401 cagtggcaaa attcacctta atattcttca tccccttttt ttcaaggtat ttttgaatgt
     2461 tcaatgcaca ggtggtgcaa tccattccat caactttcca ttgtacctgt tccatactac
     2521 aaaattaccc aaactggcaa agcctttttt ccgacaaggg gaatatgttt acaacatggt
     2581 tgctttatcg tactaaattt gccgtatgga atggaagttt tcactggatt ctataaaaga
     2641 aatagcgaca gcgttttggg aagcggccgg caatgcttct gtaattgctt tccacgggca
     2701 aatgggaacc ggaaagacga ctcttattca tgccctctgt gatgttaaag gcgtaaaaga
     2761 tgtggtgagc agcccgactt tttcaattat taatgagtat gtgtatgatt gcgaaggaac
     2821 aaagaagccg ctctttcata tggatcttta tcgtctgaaa gatgaacagg aagcacaaca
     2881 ggcgggtgtg gaagatgcct tgcacagcgg tcatatctgc ctggtggaat ggccggaaaa
     2941 agcccccggc atattcccgg ataacacctt tcatgcgtac ctggaactta ttgatagcaa
     3001 aacaagaagg ctgaaaatca gcaataatta gttatttttg aaagtagtca gcatcaactt
     3061 tcacaaccaa cttcatgagt cagcaaagac ctaaaatcag tacttctttc agctatgaaa
     3121 ccctggaaga aacacttgat ataaaaccca gggcggaagg gatgatgata ggtattccca
     3181 aagagatcgc tttccaggaa aatcgcatag cgctgacccc ggatgcggta agtgtgctga
     3241 taagcaatgg gcaccaggta atgatagaac ataatgccgg cgaagcttcc catttcaggg
     3301 acaaagatta cagtgaagca ggtgctaaga ttgtatatga cagggaaagt gtttttaaag
     3361 cgccaatact ggtaaaaagt gccccgatta tcgatgaaga tctgccgttg ctgcaattaa
     3421 atcagatcat catttctccc attcatcttg ctgtaatgaa agctgagatc ctggagaaaa
     3481 tgatggagaa aagagttacc gctatttctt ttgagaacct gaaagacgat agtgattctt
     3541 atccaatcgt tcgcagtatg agtgaaattg ccggtagcgc cgtgatgctg atcgctgcac
     3601 aatatcttgg ctccgctaat catggcaaag gtgtgttact gggtggtgta tccggtattg
     3661 ccccaacaaa agttattatc attggggcag gaatagtggg agaatatgcg gcgagggcag
     3721 ctcttgcact aggtgcttct gtgaaagtat ttgataacag tgtgtatcgg ctaaaacggt
     3781 tacagaataa tattggccag cggatgtgga cctcggttat tgagccgaga ttattagcca
     3841 aacagctcaa aacctgtgaa gtggccgttg gggcgctggg ttcagaaaca ggaagaaccc
     3901 cgttgattgt gacagaagaa atggtcagta atatgcgacc gggttctgtc attattgatg
     3961 tgagtattga ccggggtggt tgttttgaaa catcagaaat tacatcacat gagcacccca
     4021 tctttatgaa gtatggggtg atacattact gtgtacccaa tattccatcc ggttttgccc
     4081 gaactgcttc acaggctatc agcaatgtgt tgatgccttt actgcttgaa gcaggtgagg
     4141 agggtggttt tgaaaacctg gtttggcaca aattacatct ccgcagcggt atatatcttt
     4201 ttaaaggggc attgaccaat ttctatctca gccagcggtt tgatttgaag tatacggatt
     4261 taaatttgct gattgctagc cagcggtagg gaaaaaattg tcgtataatt ttttatatgt
     4321 acatttagat tataacatcc ctaagaatcc ttcttaattc tacctgtcat ttattaattt
     4381 tttaaaaaaa tcaacatgag aaatgttctg atcatcatgg cagggctgtt tgtgcttgcc
     4441 tgtaacaatc aagaagacaa aaaggccgag aagaaagtaa agtacagcga tctggttaat
     4501 gaacgtctta acggtaatgt tcaaagtgtg gaagaaactc cataccaggt ggatagcgct
     4561 ggtaagatcg ggaatatgga ttcctgttgt attgatctgg tagaatacga tgaaaacggc
     4621 aatgctgtta agtttacctc aaaggacagc aaagggacgg taaagaatca atccgtttat
     4681 acccggcatg agacaggttt atggatcggg tcaactgaca ccaaagaagg tggtaagcca
     4741 tcgggatcaa tgaaagtagg agtagatgat aaaggccagt atacagtagc ggaggctttt
     4801 gattctaccg gaaagcttga tgtttattat actgatacta aacaaaatga atttggccag
     4861 attttgggat ggaaacaata tgataaggat agtgttttca gaatgcaagg tgaagccaca
     4921 tatgacaagg gcttgcagat gggattcaca actaaagaca gtgtcggcaa attaaagtca
     4981 tccagttctt tcaagtataa tgataaggga gaacaaactg aagctttaca aaccaatgtt
     5041 ataaaagatt ccacaacgac aaaagtgaca aaatatacct atgattctca tgatgaaatg
     5101 ggtaactgga cacagcgtac tacctttgat gataaaggca aggcaataaa aattgtcaaa
     5161 agagtttata catacaggaa ggacgaagag aaaaaataag aatttggtaa tatatttcta
     5221 ttgcagatta aatgtgcccg ttagatctaa cgggcacatt tgattataag tgtatcctga
     5281 attgaatgta caaattcctt cccggtcttg gaatatttcc ccagtccagg tgctcatgat
     5341 attttctgtc aaaaatattc tcaactcctc cctgtaactc aataatatta ttaaatatca
     5401 ttgtattgta gccaaaccga atatgcaaaa gggaatagcc agttgtaata tcttccccgg
     5461 cttctgtatt tatacagtct tgtttacttg cggcttctgc ttccagttgt acagaaaaat
     5521 tctttggctg ataccggact gatgttgtat tcttcaaagg agcaataaag ggtaatggat
     5581 ttcctttatt gtcttttccg gctgtatacc ggatagtgga aaccacatca atggcagggg
     5641 aaggtttaag tatcaaactg ccttccattc ctgatacaga tgcatccggg atattgataa
     5701 attcttttac gccattggcg ccgatcgtca tagtactgaa agaagaattg atagtgccag
     5761 ttatataatc aaacaaactg gaatagaaat aactaagttg aacccggtag tttttccagt
     5821 tgtatatggc agacaaatca gcctgtagtg atttttcaat gtttagtttt ggattaccga
     5881 tataatcgta tccatcatta ctgttgaaca ggtaaaagcc atatagctca ctggctgttg
     5941 gcattctttc agtgtaagct atactggccg atacttttat cttttgagag agtttttttg
     6001 agaaaagtac tgaaagattt ttcaatagat catttctgtt agcagaaggg taaccaaaaa
     6061 tactaacctg gtctttggct tcctgggaga ctagattatg ggttataaaa tcagttctgc
     6121 cggttacctg tagtttcact gtgctgtcga tttgcattaa ccaggaagca gaaatgccgt
     6181 attgattttt tctattgtcc ggccaggtaa gcatatacat tggaagttct cctggctggt
     6241 acatggtcat cgaagctttt aaaaaagtag aagaaccgtc tgccctgagg agtagttttt
     6301 gcttttgatt tactttaagt tcagcttctg aataagcgcc ataagtttta ctccaccccg
     6361 gcatatcc
//
LOCUS       DAORTI010000166         6359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14345_length_6359_cov_6.952570, whole genome shotgun sequence.
ACCESSION   DAORTI010000166 DAORTI010000000
VERSION     DAORTI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14345_length_6359_cov_6.952570"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLO70_14885"
     CDS             <1..979
                     /locus_tag="PLO70_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ75803.1"
                     /translation="LAQKGTEITMTDLAGMMYDSIHKKLFPLANHVIVYPAHGPGSSC
                     GKNLGPNTHSTIGEEKQFNYAMKAETKEDFIKQVTDGIAPPPSYFPINAKINKEGYDS
                     LGDILLAGMKTLSIDEFKQLISNDVIVLDTRPAGTFTHGFVPGSISIGLEGRFAEWAG
                     SLLPFNVSIVLVTEPGKEKETIIRLARVGFEKIKGYLDGGYEAWENAGEPIDMIIDIE
                     ADELMMDIPHDQNLVVIDVRRETEFADGHLKDAVNIPLIEMTDPGNLANLEETQNLYI
                     HCAAGYRSVIASSLLKRQGIHNLRNIAGGWEKIKEQEKAEIIKETSVLN"
     gene            complement(1043..1750)
                     /locus_tag="PLO70_14890"
     CDS             complement(1043..1750)
                     /locus_tag="PLO70_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75804.1"
                     /translation="MSASAPAIFWPHSEHWSVKIPLKTSHYRMPVMEEKGYVIMKTLD
                     ISHIPTAEWENLAYMDWKSGGDTNFAPLASCDGTNECKGFWENGKTDKDAIFTPNAEL
                     CPNLMAYIKSIPANFGRVRIIKLEPQNHDQALRSVHRDDNNRLNPEDEGWVVRMWIEL
                     TDNPDSYMLLYDSDENNLPIKESEARVPMPKGAHFVVDSQRLWHVGVHNGDKPRYAVI
                     ISCESSPALNDWIKSKM"
     gene            2059..4533
                     /locus_tag="PLO70_14895"
     CDS             2059..4533
                     /locus_tag="PLO70_14895"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQZ75805.1"
                     /translation="MKIKTNVTLIGAMLLLCMYSNAQSQTPRQLPAKRTTQPVKIDGL
                     LNDAAWKDAAMMNDLVEFRPKMGAKENHENRSETFLMYSNDGIYFGGFLYERTKDSIA
                     TELAGRDGFGTNDYIGLIFDTYYDKINGFEYFVTPLNEQWDAKMAPASNGNNEDFSWN
                     AVWQSGAVIHDNGWSFEMFIPYSAIRFGQKEVQDWGLNITRRRRKTEQQYTWNPIDVN
                     VNGFLTQEGIWTGITNIKPPMRLQFSPYFSVYANHFPANQPGQKNWTSQVNGGMDVKY
                     GINQAFTLDATLIPDFGQVQSDNQVLNLSPFEVKFNENRSFFTEGTELFSKGNLFYSR
                     RIGGTPIHLYDAYNVSPGEVVVKNPSESKLINASKISGRTQKGLGIGILNAITKPQYA
                     TIENSTTKEQYQFETDPLTNYNILVLDQTMKHNSSISFVNTNVWRSGSDYDANVSAAL
                     FDLNDKKNMWNAGGKLAMSNLIGYKTGGKPETGLSHQLYFGKTSGRFNFNIYQERTDT
                     RFNSNDMGYFTNNNFLDHGTWVGYRWTEPKKWYNRIFLNFNANLSMLQKAIDPITQTY
                     QSANFNINSNIQTKKLWWFGGFTGYNFRQNDFYEPRREGWYFKRGSSLEIGGWVNSNE
                     SKKYSFFIEIFGSKRFNFYNSHGYNFNLGQNIRLNNKLSFSHRVSFNPQYNNIGFVHI
                     PGNADINFAKRNRNTIEHILSAKYNFTNKMGITYRARHYLSTVDNKEFYILQSNGELA
                     SNPMNYTANQNVNFFNIDMVYTWQFAPGSFINIVWKNAVVDFDGHVQKNYFKNLDRTM
                     QADENNNISLKVIYFLDYLQLKKKKS"
     gene            complement(4581..5459)
                     /locus_tag="PLO70_14900"
     CDS             complement(4581..5459)
                     /locus_tag="PLO70_14900"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HQZ75806.1"
                     /translation="MYYVVYGFLWLLSLLPFRVLHFFADVIYALVFYVFKYRREVVIK
                     NLEIAFPEKTAKERLKIAKQFYHNLIDSFIETIKFISITPKAAFKRSSADVELINQLI
                     EKGYNVHAMACHQFNWEYANILYPLKLTAPFVGVYMPITNKILDRIFYNFRKRHGTVL
                     VSATEFKSKMHQVFSGRYVLALAADQNPGDPTNAYWVNFFGRLTPFVTGPAKGAVKNN
                     TAVVMIGFRKIKRGYYHFDCKLLTEAASQFTPQQLTVIYKNEVERIIRQDPANYLWSH
                     RRWKYTWKPEYGNSIE"
     gene            complement(5478..>6359)
                     /locus_tag="PLO70_14905"
     CDS             complement(5478..>6359)
                     /locus_tag="PLO70_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQZ75807.1"
                     /translation="IQFHPTALYEPGVRGQAFLITEAVRGDGGILRNKNGDAFMERYD
                     PRKDLAPRDIVARAIDSEMKRTGTEHVWLDCRHFSKEKFVEHFPNIYEKCISIGIDIT
                     KHMIPVAPAAHYSCGGIKTDEWGRSSIKNLYACGECASTGLHGANRLASNSLLEAMVF
                     AHRCFIDVAEKVKSISFNMFLPDWNAEGTTDPKEMILITQSLKELQLIMSDYVGIVRN
                     DIRLQRASRRLDLLWEETEQLYQSSSLSPQLLELRNMITVGYLIVKGASFRKESRGLH
                     FNTDYPGKSHLVQNIVL"
BASE COUNT         2013 a         1315 c         1249 g         1782 t
ORIGIN      
        1 tcttgcacaa aaaggtactg aaataacaat gactgatctc gctggtatga tgtatgatag
       61 tatccacaaa aaattattcc cgttggcaaa tcatgtaatt gtttatcctg cccacggtcc
      121 gggtagttcc tgcggaaaaa atcttgggcc gaacactcac agcactattg gtgaagaaaa
      181 acagtttaac tatgcaatga aagctgaaac aaaagaagac tttatcaaac aggttacaga
      241 tggaatagct cccccgccat cctattttcc gattaatgct aaaataaata aggaaggata
      301 tgatagtctc ggcgacatac tcttagcggg aatgaaaacg ttgtccattg atgaattcaa
      361 acagcttatc agtaatgatg tgattgtgct ggacacccgg cctgcaggta cgttcaccca
      421 tggttttgta cctggctcaa taagcattgg cctggaagga cgatttgcag aatgggccgg
      481 tagtcttctc ccttttaatg tgtccattgt tttagttaca gaacccggaa aagaaaaaga
      541 aacgatcatc cggttagcca gggttggttt tgaaaaaatc aaaggctacc tggatggtgg
      601 atatgaggcc tgggaaaatg ccggtgaacc catagacatg atcattgata tagaagctga
      661 tgaactaatg atggatatcc ctcatgatca gaacctggtt gtaattgatg tacgtcgtga
      721 aaccgaattt gcagacgggc atttaaaaga tgcagtaaat atccctttaa ttgaaatgac
      781 cgatcccggc aacctggcca acctggaaga aacccaaaac ctgtatatac attgcgccgc
      841 aggctaccgc agtgtgattg catcctcctt attaaaaagg cagggcatac acaatctcag
      901 aaacatagca ggcggatggg agaaaattaa agaacaggaa aaagctgaga taataaagga
      961 aaccagtgtg ctgaactaat gattacttcg attaaaaagc cctgttatca taaaaaataa
     1021 cagggcttta ctattttaaa acttacattt tagatttaat ccagtcattc aaagccgggg
     1081 aactctcaca gctgatgatc actgcatatc ttggcttatc tccattatgt acacccacat
     1141 gccataatct ttgtgaatcc actacaaaat gtgctccttt aggcataggc acccttgctt
     1201 ctgattcttt aattggcaga ttattttcat cgctatcata caaaagcatg taactatccg
     1261 ggttatcggt cagttcaatc cacattctta ccacccagcc ttcatcttca ggattaaggc
     1321 gattgttatc atcccgatga acggaacgga gagcctggtc atgattctgt ggctccagtt
     1381 taatgatacg aacccttccg aaattggcag gaatagattt aatataagcc atcagattag
     1441 gacataactc agcgttggga gtaaaaatgg catctttatc tgtcttcccg ttttcccaaa
     1501 atcccttaca ctcattggta ccatcacagg aagccagtgg tgcaaagttg gtatcgccgc
     1561 cacttttcca gtccatataa gcaaggtttt cccactcagc ggtggggata tgactgatgt
     1621 ctaatgtttt cattattaca tacccttttt cttccataac aggcatgcgg taatgagaag
     1681 tttttaatgg gatttttaca gaccagtgtt cactgtgtgg ccagaaaatt gcaggagctg
     1741 aagctgacat agtaggtttt aattaatagt tgaaaaatgt agtccgctaa agtaggaaat
     1801 atcagattaa ttaaaaatga taacatggaa tctcatcagt atttcagctc cggcaaaatt
     1861 ttcacaatga aaagttaaca ccccgctttt ttaaccattc gtcaggaatt tgtcaccggt
     1921 cagataattc agggtaaaaa aggattatca ggccattact tttatcctgc tattgattta
     1981 atagttagtg ccgcacacca aactaaaatg taacaaatca tacatttttt gcgtcataac
     2041 cactgttaaa gtatagtcat gaaaatcaaa accaatgtaa ccctcatagg ggcaatgctg
     2101 ctcctatgca tgtacagtaa tgcacagtcc cagacaccac ggcaattacc tgccaaacga
     2161 accacacaac ctgtaaaaat tgatggcctg ctgaatgatg ccgcctggaa agacgctgct
     2221 atgatgaacg accttgtaga atttcgtccc aaaatgggag ccaaagaaaa tcatgagaac
     2281 cgttctgaaa cctttttaat gtatagtaat gacggtatct actttggtgg cttcctgtat
     2341 gaaaggacaa aagatagcat agctactgaa ctagccggtc gtgacggttt tgggaccaat
     2401 gattatatag gtttgatttt cgatacttac tatgataaga tcaacggctt tgaatatttt
     2461 gtaacgccgc tgaatgagca atgggatgct aaaatggcac ccgcttccaa tggcaataat
     2521 gaagatttct cctggaatgc agtatggcag agtggtgctg tgattcatga taatggctgg
     2581 agttttgaaa tgtttatccc ttattccgcc atccgatttg gtcagaaaga agtacaggac
     2641 tggggattaa atatcacccg caggagaaga aaaacagagc agcaatatac ctggaaccca
     2701 atagatgtta atgtaaatgg tttccttacc caggaaggta tctggacagg gataaccaat
     2761 atcaaacccc cgatgcgtct gcagttctct ccttatttct ctgtatatgc taatcacttc
     2821 cccgcaaatc aaccgggaca gaagaactgg acgagccagg taaatggcgg aatggacgtg
     2881 aagtatggca ttaaccaggc ctttacatta gatgcaacat tgattccgga ttttggccag
     2941 gtgcaaagcg ataaccaggt cctgaacctc agcccgtttg aagtaaagtt taatgagaac
     3001 agaagtttct ttaccgaagg aacagagtta ttcagtaaag gcaacctgtt ttattcaaga
     3061 agaattggcg gtactcctat tcacctttat gacgcctaca atgtatcacc aggtgaagtg
     3121 gtagtaaaga acccatctga atccaaactg attaatgctt caaaaatttc cggacgaacc
     3181 caaaaaggac taggtatcgg tattttaaat gcaattacaa aaccccaata tgctaccatt
     3241 gagaactcaa caaccaagga acaataccaa tttgaaacag acccgcttac taactataac
     3301 attctggtgc ttgatcagac aatgaaacac aattccagta tctcctttgt taataccaat
     3361 gtttggcgta gcggatctga ttacgatgcg aatgtcagcg ctgccctgtt tgatctcaat
     3421 gacaaaaaaa atatgtggaa tgcaggtggg aaactggcaa tgagcaattt aattggttat
     3481 aaaaccggag gaaaacccga aacaggatta agccatcaac tctattttgg caagaccagc
     3541 ggccgcttca atttcaatat ttaccaggaa agaactgata ccagattcaa tagtaacgat
     3601 atgggctact tcactaacaa taacttcctt gatcatggca catgggttgg ctacaggtgg
     3661 acagaaccta agaaatggta taaccgcatt ttcctgaatt ttaatgccaa tctgagtatg
     3721 ttgcaaaaag ccattgaccc aataacccag acttaccagt cagcgaattt taatatcaac
     3781 agcaatattc agacaaaaaa actatggtgg tttggaggat ttacaggtta caatttccgt
     3841 cagaatgatt tttatgagcc ccgccgggaa ggatggtatt tcaaaagagg ttccagcctg
     3901 gaaattggcg gctgggttaa ttccaatgaa tcaaaaaaat attcattttt cattgaaatt
     3961 ttcggaagta aaagatttaa tttttacaat agccatggat acaattttaa cctggggcaa
     4021 aatataagac tcaataataa attatcgttc tcacaccggg taagcttcaa tccgcagtat
     4081 aataatatcg gttttgtaca tattccgggt aatgcagata tcaattttgc caaaagaaac
     4141 agaaatacca ttgagcatat acttagtgcc aaatataatt tcacgaataa aatggggatt
     4201 acgtacaggg caagacatta cctgagtacc gtagacaata aggaatttta tatcctgcaa
     4261 tcaaacggtg aactggcttc aaaccccatg aactataccg ccaatcaaaa tgtgaatttc
     4321 ttcaacattg atatggtata tacctggcaa tttgccccgg gaagttttat aaatattgta
     4381 tggaagaatg ctgtcgttga ctttgacggt catgtccaga aaaattattt taagaacctt
     4441 gacagaacta tgcaggcaga tgaaaacaat aatatttcat taaaagtgat ctatttcctg
     4501 gattacctgc aattgaagaa aaagaaaagt tgattaagca gtacttaaag tgcaccctgg
     4561 ttttcaggtg gcactttaat ttattcaata ctatttccat attcaggctt ccacgtatat
     4621 ttccaccggc gatggctcca cagataattt gcaggatcct ggcgaattat cctttctact
     4681 tcatttttgt atatcactgt aagctgctga ggtgtaaatt gtgatgctgc ttcagtcagc
     4741 agtttgcagt caaaatgata ataccctctt ttaatttttc taaacccgat catgaccacc
     4801 gctgtattat tctttacagc tccttttgcc ggtccggtaa caaagggagt aagtctgcca
     4861 aagaaattta cccaatatgc attagtagga tctccgggat tttgatctgc agcaagggcc
     4921 aatacatagc ggccagaaaa aacctggtgc atcttacttt tgaattcagt tgctgatacc
     4981 agcactgttc catgcctctt cctgaaatta taaaatatac ggtccagtat tttgttggta
     5041 atgggcatat acacacctac aaacggagca gttagcttca agggatacaa tatgttagca
     5101 tactcccagt taaactggtg acaggccatt gcatgtacat tatagccttt ctcaataagc
     5161 tggttgatca attctacatc agcagagctt cttttaaaag cagctttcgg tgttatagaa
     5221 ataaatttaa ttgtctctat aaatgaatcg atcaggttat gatagaattg tttagcgatt
     5281 tttaatcttt cctttgctgt cttttccggg aacgcaattt caagattttt tatcaccacc
     5341 tctctcctgt atttaaatac atagaatacc aatgcataaa taacatcagc aaaaaaatgt
     5401 aagacccgga aaggcagtaa agaaagcagc cagagaaaac catatacaac ataatacata
     5461 gcgggcaaag ataaagacta taaaacgatg ttctgtacaa gatgactctt gcccggatag
     5521 tctgtattaa aatgcaggcc gcggctttcc ttccggaaac tggcaccctt aacgatcagg
     5581 tagccaacag tgatcatatt acgaagttcc agcaactgcg gtgagaggga gctgctttga
     5641 tacaattgct ctgtttcctc ccacagcaaa tcaagccggc gtgaagccct ttgtaaacgg
     5701 atgtcattac gcacaatgcc tacataatca ctcattataa gctgaagctc tttcaggctt
     5761 tgtgtaatca ggatcatctc tttgggatct gtagtacctt ccgcattcca gtcaggcaaa
     5821 aacatattaa atgaaatact ctttactttt tcagcaacat caataaaaca acgatgagca
     5881 aataccatcg cttccagcaa tgaattactg gctaaacgat tggccccgtg caaaccagta
     5941 ctggcgcatt cgccgcaggc atacaaattc ttaatggaac tcctgcccca ttcatctgtt
     6001 ttaattccac cgcagctgta atgtgcagca ggcgctaccg gtatcatgtg tttggtaata
     6061 tcaatgccta tggaaatgca tttttcgtaa atattaggga aatgctccac aaatttttct
     6121 ttgctgaaat gccggcaatc aagccataca tgctctgtac cggtgcgttt catttcacta
     6181 tcaatagccc tagccacaat atcacgggga gccaggtctt ttcggggatc atatcgttcc
     6241 ataaaagcgt ccccgttctt atttcgtaat atccccccat caccccgtac agcttcagta
     6301 attaaaaatg cctgtcctct tactccgggt tcatacaagg cagtgggatg aaactggat
//
LOCUS       DAORTI010000167         6310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14512_length_6310_cov_4.490967, whole genome shotgun sequence.
ACCESSION   DAORTI010000167 DAORTI010000000
VERSION     DAORTI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14512_length_6310_cov_4.490967"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1334
                     /locus_tag="PLO70_14910"
     CDS             222..1334
                     /locus_tag="PLO70_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75808.1"
                     /translation="MKKILLLLLVILSATICTIAQNVGIGTPAPESKLHVVDTFNIAD
                     GSSGAFINVHNATLMAPTGTLSGIRFRLDGVNAGANARYKGGIFFEKTGSFGVGKLHF
                     LTNSLGNNNSITTADARMTIAADGKVGIGNSSPSLAQLEINGPSNSALLTVRNGVDLP
                     GISQSVPSNAFPTLGFNLYYNNSYRFLGNGYGANIQYNPNLGVLSFSSSQTKGIPGET
                     SLFNSGSLFLDSSGNVGVGTSVPKAKFHVTTGMVIGTSSTTPATGYILSVNGKIMSEE
                     VRVQMDANWPDYVFDNKYKLRPLKEVEQFIAAKKHLPGIPSAKQVAEDGLHLGDMQKR
                     IMEKVEELTLYVIQLNKENQQLKQEVETLRKIVENK"
     gene            1361..4195
                     /locus_tag="PLO70_14915"
     CDS             1361..4195
                     /locus_tag="PLO70_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75809.1"
                     /translation="MKKICILFLLLTGLQATAQVKIPAEVAQRLQGNKKFTDYAREMM
                     QYVTSNQSRFPAGSKERTYFEKQEKALARHLWYLEGRQEANGDIAEYTRKTFSAVQQY
                     EANRAAQRIEATAYGSWSLVGPTNVSTSTANLHTGIGRVDRIAFHPTDPNIIFAGTPA
                     GGVFKSNTGGNSWFNLNSFMPSLGVSGLVVSWADANDIYVLTGDGDSNAGDNWFVQGF
                     DYIRPSIGVLKSTDGGATWQRTGDLGLTGFYVGYKLVQSPTNANVLIAATSQGLFRTA
                     NGGNTWTLVSPNSNRYYDIEWKPGSSTTVYACTGGVFYISTDGGVTFENSNANFDFSL
                     YGQRTAIAVTPANANYVYVFANNGGANVGVFRSTNSGTSFTRRSNSSTITVDSAPYMH
                     NIAVASDNVNIVITGELEIYRSTDGASTFSLSNQRGDATQTDYAHDDIHELAYNPLDH
                     SLYVGSDGGVYKSTDDGVTYVAKYTGMSNTQYYHFDVSDTDENYIFGGAQDNGGHYRN
                     GATATFQHTIKGDGYDVRFYNGSTSQVYLSVNKKLFRSNATLTDWTEMPGMTQDWYKT
                     VAMSYSSNNIVFTSSDPVYRTTNSGAGWTNIGANGRWAMVTCPSNSNRVYAAGGDSWN
                     DGGTQAGKKLFRSDDQGDTWVELQENTGFPETITKITGIGVDPGNSLRLWVTMGGFTD
                     GEKVYYSNNGGDTWVNLSGTIPNVPVNCITIDANLDAYIGTDAGVFFKGALMPDWQPF
                     YNFLPRVPVTELHIRNGRIYASTFGRGIWRSDTHGDCPPSINFVGSLQGVKFYEALVI
                     NSDNTLIGGAGTEIFLRAQDYVSLTEGFLANASTGESFRAWIGNCASGGIPTMRNSDI
                     SGWQAARSNSNRLAKKEEGDEITYSLDMPFDGKASLFLMDADAKPKEVLLLNRMLKKG
                     INAVSLSKTERQPGTRMVLFIDGQLEGVIE"
     gene            complement(4378..5133)
                     /locus_tag="PLO70_14920"
     CDS             complement(4378..5133)
                     /locus_tag="PLO70_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ75810.1"
                     /translation="MIRILIVDDEPSARNILQMLIEKYIAGNKEIKTCASPEEALETI
                     PVFKPTLLMLDIEMPHMNGFDLLNRIGHWDFDVIFTTAYDKYAIKAIRFSALDYLLKP
                     VDIVDLQNAVNRHIVKKEYPPQQEKLVTNLINNLQQKDASSFKLALSTMEGVFFFEPA
                     AIIRCEGENNYTRFFFPDRRPLLVSKTIKEYEEILGEYGFLRAHKSHLVNKTFVRHLD
                     KEGLLWLTDGSNIVVSRRKKEEVMKELMNKPKS"
     gene            complement(5130..>6310)
                     /locus_tag="PLO70_14925"
     CDS             complement(5130..>6310)
                     /locus_tag="PLO70_14925"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQZ75811.1"
                     /translation="QKNGTIWGLTSMGLVSFHPDSISRNDLPPVLLINTIAVSGSTGN
                     FFNIKSPVLSYDHNSIQFHYTAICLTDPSKIRYAYRLKELDSNWVYTNNRNVDFNFLQ
                     AGTYTFELKACNNNNVWTSQPLQYVFIIRPPFWQTWWFGVILLSMITAVIFSIFKLRM
                     RSFKTKAAIRQQMAELEAKAIRAQMNPHFIFNSLNAIQELIITENYTAAYQYLSKFSK
                     LLRLVLNNSEKNLIPLRNEIEMNQLYLELESLRFKQSFHYTIRVDEKIDADTVLFPSL
                     LLQPFIENAIWHGLMHKEGDKKLAVSFIEENNRLRCFVEDNGVGRIKAAEIKARKLGA
                     HHFDSKGTQLALQRIEALQQTGFTGAEIKTEDIFDGEGNNSGTKVEINIPLPKKTTTP
                     "
BASE COUNT         1817 a         1226 c         1434 g         1833 t
ORIGIN      
        1 aaaaatgaga atcactgtca gggttttaaa tccatgcggt ggtaaattat ttccgataat
       61 agcagagcct ctttatgttt tggccggatt caccatccaa ggttacgcaa caatgtttta
      121 tgggagctag ccctggagca tcacatcatt agggtattgc aattacatct aagttcttta
      181 tttttatcaa gatcaatcat cagaaattat aaactatgta tatgaaaaag atcctcttgc
      241 tgctgctggt aatactgtct gccaccattt gtaccattgc acaaaatgta ggtatcggta
      301 ccccggctcc ggaatcaaaa ctacatgtgg tggacacgtt taatatagcc gacggtagca
      361 gcggcgcctt tatcaatgtg cataatgcca cacttatggc tcccaccggt acactcagcg
      421 gtatacgctt tcgcctggat ggggtgaatg caggtgccaa tgcccgctat aaaggtggaa
      481 tattttttga gaaaactgga agttttgggg taggcaaact tcactttttg acgaatagtc
      541 ttggaaataa taatagtatc actacagccg atgcccgtat gaccattgcc gctgatggaa
      601 aagtggggat aggtaacagt agtccttctt tagcacagct ggaaataaat ggtccttcta
      661 attctgcttt acttacagtt cgtaatgggg ttgatctgcc gggcatcagt cagtctgttc
      721 cttcaaatgc atttcccaca ctagggttta atttatatta taacaattcc tatcgttttt
      781 taggcaacgg ttatggagct aatattcagt ataatcctaa cctcggagta ctttccttca
      841 gtagttctca aactaagggt atcccgggtg aaacaagtct ttttaatagt ggctctctct
      901 tcttagacag tagcggaaat gtgggtgtcg gtacttctgt acccaaagcc aaattccatg
      961 taacaacggg aatggtgatt ggtaccagca gtaccacacc agccaccggt tatattttat
     1021 ccgtaaatgg aaagatcatg tctgaagaag tgagagtgca aatggatgcc aactggccgg
     1081 attatgtatt tgataataaa tacaaattga gacctttaaa ggaagttgaa caattcatcg
     1141 ctgctaaaaa acatttaccc ggaatacctt ctgccaaaca agtggcagaa gatggccttc
     1201 acctgggaga catgcagaaa agaataatgg aaaaagttga agagctcact ctctatgtga
     1261 tccagctgaa caaagagaac cagcagttga agcaggaagt agaaacactg cgtaaaatag
     1321 tagagaataa ataacgcttc ttcttcataa aaaaagcaag atgaaaaaaa tttgcatact
     1381 attcctgtta ctgacagggc tacaggctac ggctcaggta aaaattccgg cagaagtggc
     1441 acaacgactc caggggaata aaaagtttac tgattatgcc cgtgaaatga tgcagtatgt
     1501 gaccagtaac caatctcgtt ttccggcggg ctcaaaggag agaacttatt ttgagaaaca
     1561 ggaaaaagcc ctggctcgtc atctttggta tctcgaaggc agacaagagg cgaatggtga
     1621 tattgctgaa tataccagaa aaacattcag tgctgttcaa caatatgagg caaacagggc
     1681 agcgcagcgc attgaagcta cagcatatgg cagttggagt cttgtagggc ctaccaatgt
     1741 ttccaccagt actgcgaatt tacataccgg cattggccgg gtagaccgta ttgcttttca
     1801 tccgacagac cccaatatca ttttcgccgg cactcccgcc gggggagtct ttaaaagtaa
     1861 taccgggggt aacagttggt ttaacttaaa ttcatttatg ccctctctcg gcgtttccgg
     1921 attagtggta agctgggctg atgccaatga tatttatgta ttgacagggg atggggatag
     1981 taatgctggt gataattggt tcgtacaggg atttgattat atccgtcctt ctattggggt
     2041 attgaaatct accgatggcg gagcgacctg gcaacgtacc ggtgatcttg ggttgactgg
     2101 tttctatgtg ggctacaagc tggtgcaaag ccccactaat gcaaatgtat taattgctgc
     2161 aaccagccag gggttattcc ggactgctaa tggtggcaat acctggacat tggtttctcc
     2221 aaacagcaac cggtattatg atatagaatg gaaaccgggc agtagtacaa cagtgtatgc
     2281 atgtacggga ggtgtctttt acatatcaac ggatggagga gtcacatttg aaaacagtaa
     2341 tgcaaatttt gatttttcat tatatgggca gcgtacagca atcgctgtta cacccgcaaa
     2401 tgctaattat gtatatgtat ttgcaaataa tggtggggct aatgtaggcg ttttcagaag
     2461 tacgaatagc ggtacgagtt ttacaagaag aagcaattcc agcactatta cggtagactc
     2521 tgccccctat atgcacaata ttgctgtggc ttcagataat gtgaatattg ttattacagg
     2581 tgaattagaa atataccgta gtaccgatgg agcttctact ttttcactct ctaatcaacg
     2641 gggtgatgcc actcaaacag actatgcaca tgatgatata catgagctgg catataatcc
     2701 actggatcat tcactttatg taggttcgga tggtggtgtt tataaaagca ccgatgacgg
     2761 agtaacttat gtggccaagt acacaggaat gagcaataca cagtattatc attttgatgt
     2821 gtctgataca gatgagaatt atatttttgg cggggcgcag gataatggag ggcactaccg
     2881 caacggagct accgctactt tccagcatac aatcaagggc gatggttatg atgtgcgttt
     2941 ttacaatggc tctaccagcc aggtttattt atcggtcaat aaaaaattat tccgttccaa
     3001 tgctactttg acagactgga ctgaaatgcc cggcatgacg caagactggt ataaaacagt
     3061 tgccatgtcc tattccagta ataatattgt ttttacgagc agcgaccccg tctatcgtac
     3121 cactaattca ggagctggct ggactaatat aggagccaat ggacgatggg caatggtaac
     3181 ctgcccaagt aacagcaacc gggtttatgc tgctggtgga gatagttgga atgatggtgg
     3241 tacgcaagcc ggaaagaaac tattccgcag tgatgaccag ggagacacct gggtagagct
     3301 ccaggaaaat actggttttc cggaaactat caccaagatc accggtatcg gtgttgatcc
     3361 gggtaattca cttcggttat gggttaccat gggtggcttt accgatggtg aaaaggttta
     3421 ttacagcaat aacgggggcg atacctgggt caatttatca ggtacgatac ctaatgtacc
     3481 cgtcaattgt attacgattg atgcaaacct ggatgcctat atcggtacgg atgcaggtgt
     3541 ttttttcaaa ggtgcactaa tgcccgactg gcagccgttt tacaattttt tgccacgggt
     3601 acccgtcact gaattgcaca taagaaatgg caggatctat gcatctactt ttggccgtgg
     3661 tatctggagg tcagatacac atggggattg cccgccatcc attaattttg ttgggagcct
     3721 gcagggagta aaattttatg aagcacttgt tattaatagc gacaatacat tgattggtgg
     3781 agcaggcaca gaaattttcc tccgggcaca ggattatgtg tcattgacag aaggctttct
     3841 tgccaatgcc agtaccggtg aaagcttccg ggcatggata ggtaattgcg cttccggcgg
     3901 aataccgaca atgcgtaaca gcgatatatc cggttggcag gctgcccgtt ctaacagcaa
     3961 caggttagca aaaaaagaag aaggagatga aattacttat tcgctggata tgccttttga
     4021 cgggaaggcc agtctttttt taatggatgc ggatgccaaa cccaaagaag tactgttgct
     4081 gaaccgtatg ctgaagaaag gtataaacgc agtgtcctta tcaaaaacag aacgacaacc
     4141 tgggacaagg atggtactct ttatagacgg tcagttggaa ggcgttattg aataaatgag
     4201 atagatgaat ggaatattat cagcgaacta atcgtaacaa cgaccttcaa ttatttaaag
     4261 aaacgggata ttgaaatcgt tggattacct aagggataat agcgtatggg tttaatttta
     4321 tattaatgaa ttgaaagagt tgccggctca attcacaaga agtaaaatca ggatatttta
     4381 agatttcggt ttattcatta attctttcat tacctcttct ttcttgcggc gggatacaac
     4441 tatattactg ccatctgtca gccatagcaa accttctttg tctaaatgcc tgacaaatgt
     4501 tttattgaca aggtgtgatt tatgcgctct caggaaaccg tattcaccaa gtatctcttc
     4561 atattctttg atggtttttg ataccagcaa tggtcttctg tcagggaaaa agaaacgggt
     4621 ataattgttt tcaccttcgc aacggataat agcagcaggc tcaaaaaaga aaacaccttc
     4681 catagtgctc agggccagtt taaaggagga ggcatctttt tgctgcagat tattgatgag
     4741 atttgttacc agcttttcct gttgcggggg atattctttt tttacgatgt gccggtttac
     4801 ggcattttgc aggtctacaa tgtctacagg ttttaataaa taatcaagtg cactgaaccg
     4861 gatagctttt atcgcatatt tatcataggc tgtagtaaag atgacatcaa aatcccaatg
     4921 accgatccgg ttgagcaggt caaagccatt catatgaggc atctcaatat ccagcatcaa
     4981 taaagtgggt ttgaaaacag gaatcgtttc cagggcttct tccggtgaag cacaggtttt
     5041 gatttccttg ttacctgcaa tatatttttc tatcagcatt tgcaggatat tcctggcaga
     5101 cggttcatca tctacaatca gaattcgtat catggggttg tagttttttt aggtaagggt
     5161 atatttattt ccacttttgt accggaattg tttccttccc cgtcaaagat gtcttctgtt
     5221 tttatttcag cacctgtaaa acctgtttgt tgtaatgcct caattcgttg cagtgcaagc
     5281 tgggttcctt ttgaatcaaa atgatgggcc cccagttttc gggcttttat ttcagcagct
     5341 tttatccggc ccacaccatt gtcttctaca aaacaacgta accggttatt ctcttctata
     5401 aatgaaactg ccagtttttt atctccttct ttatgcatca gcccatgcca gatggcattt
     5461 tcaataaacg gttgcaacag cagcgaaggg aataatacag tgtcggcgtc tattttttca
     5521 tccacccgga tagtataatg aaaggactgc ttaaaccgga gtgattccag ttctaagtaa
     5581 agctggttca tttcaatttc atttctcagg gggatcaggt tcttctccga attattcaat
     5641 accagccgga gtaatttgga aaattttgac aggtattggt aagcagctgt atagttttct
     5701 gtgatgatca gttcctgtat cgcattcagg ctgttaaata taaaatgagg gttcatctga
     5761 gcccggatgg ctttggcttc cagctctgcc atctgttgcc ggatcgctgc tttggtttta
     5821 aaacttcgca tccgtaattt gaaaatagaa aagatgacag ccgttatcat tgataatagt
     5881 attaccccga accaccatgt ttgccagaag ggcggacgaa taataaatac atattgcagg
     5941 ggctggctgg tccacacatt attattatta caggctttta gttcaaaggt gtaagtgccg
     6001 gcttgtaaaa aattaaaatc aacattccgg ttattggtat atacccagtt actgtccagc
     6061 tcctttaaac gataggcata ccgtattttg gaaggatcag tcaggcaaat agccgtataa
     6121 tgaaattgta ttgaattgtg atcatatgaa agtacaggcg actttatgtt gaaaaaattt
     6181 ccggtagatc cgcttacagc aatagtatta atgagaagaa caggaggaag atcattgcgg
     6241 ctgatgctat cgggatgaaa tgaaaccagg cccatcgatg taagtcccca gatagtacca
     6301 ttcttttgtt
//
LOCUS       DAORTI010000168         6262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14697_length_6262_cov_7.526663, whole genome shotgun sequence.
ACCESSION   DAORTI010000168 DAORTI010000000
VERSION     DAORTI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_6262_cov_7.526663"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLO70_14930"
     CDS             <1..581
                     /locus_tag="PLO70_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75812.1"
                     /translation="RDLHDEMGSTLTSINILSKVGMESGQVSEELRSYLQKIKDNSGK
                     MMESMSDIVWVINPANDSLEKVLIRMKEFAAEMLEPARINYYFDTEGSLDQSLLNLEQ
                     RKDLYMIFKEAINNAVKYSAATEINITLQQHDQRLRLTVTDNGKGFDTASAYAGNGLK
                     NMQSRAAAMQAELRITSILGAGTTVLLEKQVT"
     gene            651..1091
                     /locus_tag="PLO70_14935"
     CDS             651..1091
                     /locus_tag="PLO70_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75813.1"
                     /translation="MPVNIIIFEDNTNLRESLVNLLALTGDYNVVASFPDCTTVIQQV
                     KEWKPEVILMDIDMPGINGIEAVKKIRTIDPAVQIIMLTVFDDNTHVFEAMYAGANGY
                     LLKKYVSDKLIQSIREVLDGGCPDEPFHCPDGDLQYAKRDSHCR"
     gene            1063..1287
                     /locus_tag="PLO70_14940"
     CDS             1063..1287
                     /locus_tag="PLO70_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQZ75814.1"
                     /translation="MQKGIPIADNDYQLTNREKEILQLLSRGNSFKMIAADLCISLDT
                     VRTHIKRIYDKLHVRSQIEAVSKAISEKLV"
     gene            1405..2490
                     /locus_tag="PLO70_14945"
     CDS             1405..2490
                     /locus_tag="PLO70_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75815.1"
                     /translation="MKKISLLFLFVLPGALFFDVTAQNVGIGNTTPGDRLHVTGGKLR
                     LEDTNYPWLSFVNNNTLQGFIGAEGVNMRVGTWPATNPTGKIFLRTNSLDRVTIDEAG
                     DVGVGVTAPLSRLHIKGGMRLESTNNTTGAIFNLYGGTDNLSYIQFYKNLTTPTTMGY
                     IGFSGSGDYSIWSRGSYAVLNSDGLGINNSAPLTRLHISSGQDAGMSNTSNGYIMLGS
                     GTSSNIILDNNEIMARNNGVIAPLTLQNDGGSVRIGNVAAPAGYLFAVNGKMISEELK
                     VQLSGNWPDYVFKKDYQLKSFDELRKFIAENNHLPNIPPAAEVEKNGLEVGDMQKKMM
                     EKIEELTLYVLQLEEQLKEIKKKISDQ"
     gene            2500..3384
                     /locus_tag="PLO70_14950"
     CDS             2500..3384
                     /locus_tag="PLO70_14950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQZ75816.1"
                     /translation="MLRINSFLAVLSFSALQLAGQPQFELSRNVYRVPYSNGITVTVN
                     NDHYTHDPLGRYDLKGSGFGGSCGVAYPIVAAAEGIIRRIVDNNNVHGPDCSSNCTQY
                     NNYVWIEHANGEWTKYSHMKYQSTTIDAGLSVGDQVCAGTFLGYECDIGQATGPHLHF
                     EVRHPNDTANVQISTSGGFMSDADHRIPVINTISKHYLSDGDSWTASGSTACTSTDIA
                     LILATTISNNGIRAYMASGTITTFAGIGFTFQNGSNGLFQAGGSVTLSPGFTASAGSY
                     FEAKIGSCATTNMVGACN"
     gene            3713..3973
                     /locus_tag="PLO70_14955"
     CDS             3713..3973
                     /locus_tag="PLO70_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ75817.1"
                     /translation="MSPSIARMVISNMQKGIPIADNDYQLTNREKEILQLLSRGNSFK
                     MIAVDLCISLDTVRTHIKRIYDKLHVRSQIEAVSKAISEKLV"
     gene            4098..5696
                     /locus_tag="PLO70_14960"
     CDS             4098..5696
                     /locus_tag="PLO70_14960"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQZ75818.1"
                     /translation="MRYKIPVFIIFFFSLVLTHAQNVGIGTERPLSKLQVDGSFSARS
                     LYSFSSSSPTAGQTYSMINGNTVQIPDTDSVARLYDHGGMAGNYLPNLTGNTLINLNG
                     TIDSYMEAVIESVNLGTGDSLIIYDNSYASPGEVLLAVGNGYTATNLSLTFSSNVGYC
                     SFKSNADASVGSGFSILFTRKFPKLTFLDIANITQKAMTYSPNLAAFRVGKISNASIG
                     WYSVAMGRNNTASGQVAIALGDRTEATNGSATAMGSQTKASGQYATAMGYQTQATDVY
                     STAMGRASWATGSAATAMGERTTASGTAATSMGYYTVASGESATAIGDGALASGVTAM
                     ASGYYTVASGRYATAMGAETHANSYASLAIGRFNDAPASSANSWVSTDRAFVIGDGTS
                     DAARSSAFIIYKNGNGWMQGALSQSSDARLKKNIHKLDQALDKINRLNGYNYYWKDEG
                     NIPGLQSGLLAQEVQQEMPELVTTNTEGELAVNYNGMVPYLLEAIKTQQQQIQTQQEQ
                     ISLLKKDVEQLKQTKKKSRKKVYR"
     gene            5817..>6262
                     /locus_tag="PLO70_14965"
     CDS             5817..>6262
                     /locus_tag="PLO70_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75819.1"
                     /translation="MKRLILFSFLLFSTVLLYAQNVAINNDGSQPHASAALDIKATDK
                     GLLLPRVANPAGAVSNAAEGLLLYNTTTQQPNFFNGTNWQGMGGALSARFPRAIAFRG
                     DVNSANSQTNATWTVPANITQVWVEMWAAGDAGQILSSAVASDMSDP"
BASE COUNT         1880 a         1250 c         1471 g         1661 t
ORIGIN      
        1 ccagggacct gcatgatgaa atggggtcaa cactgaccag tataaatatc cttagcaagg
       61 taggaatgga atccgggcag gtaagtgaag aactacgatc gtacctgcag aaaataaaag
      121 ataactccgg taagatgatg gaaagtatga gcgatattgt ctgggtcatc aacccggcaa
      181 atgacagcct cgagaaagta ctgatccgca tgaaagaatt tgcagcagaa atgctggagc
      241 ctgcccggat caattattac tttgacacgg aaggatcttt ggaccaatcc ctgctgaacc
      301 tggagcagcg gaaagacctg tatatgatct ttaaggaagc gatcaataat gcagttaaat
      361 acagtgcggc cacagagata aacataacac tgcagcagca tgatcaacga ttgcgattaa
      421 ctgttacaga taatggaaag ggatttgata cggcatccgc ttatgcaggt aatggattga
      481 agaatatgca aagccgggcc gcagcaatgc aggcagaact gaggatcact tctattttag
      541 gcgcaggtac aactgtgtta cttgaaaaac aggtcacatg attagggtat ataataaatg
      601 aaaaatctgc attatgttgc aggattactg cacattcaaa cctgggatat atgccagtca
      661 acatcatcat atttgaagac aacaccaatc tccgggaaag cctggttaac ctgctggcat
      721 tgactggtga ttataatgtg gtggccagtt tcccggattg taccactgtt atacaacagg
      781 taaaagaatg gaaacccgaa gtgatattga tggatattga tatgccgggg atcaatggga
      841 ttgaagcggt aaaaaaaatc cggacgatcg atcctgctgt tcagatcatc atgctcaccg
      901 tatttgatga taacacccat gtatttgaag ccatgtatgc aggtgccaat ggctacctgc
      961 tcaagaaata tgtttcagac aagctgattc aatctatacg ggaggtgctg gacggggggt
     1021 gccccgatga gcccttccat tgcccggatg gtgatctcca atatgcaaaa agggattccc
     1081 attgcagata atgattacca gcttaccaac cgggagaaag aaatattgca attgctctcc
     1141 aggggtaata gttttaaaat gatcgctgct gatctgtgca tcagcctgga tactgtccgc
     1201 actcatatca aacgcattta cgacaaactg catgtacggt cacaaataga agccgtaagt
     1261 aaagcgatca gtgaaaagct ggtataaatt ctccgttact tagttattct gttgtcattt
     1321 aattgttggc tggtatcaca tcattgcggt attgtgcagc taaatttctg tccccaactt
     1381 tatactgaaa acaaaactgt ttttatgaaa aagatttctt tgttgttctt gtttgtattg
     1441 cctggtgccc ttttttttga tgtaacggcg cagaatgttg gtatcggtaa tacaacacca
     1501 ggtgacaggc tacatgtaac aggggggaag ctccgcctgg aagataccaa ctatccctgg
     1561 ttatcatttg taaataacaa tacgttgcag gggtttattg gggcagaggg tgtgaatatg
     1621 cgggtaggta cctggccggc aacaaatcca acgggtaaaa ttttccttcg gaccaattca
     1681 cttgacaggg ttactattga tgaagctggt gatgtaggcg ttggagtgac agccccgcta
     1741 agccggttgc atataaaagg cggaatgagg ctggaatcaa ccaataacac taccggtgca
     1801 atatttaatt tatacggcgg aactgataac cttagttata ttcaatttta taaaaacctc
     1861 acaaccccca caacgatggg atatatcggt tttagcggaa gcggagatta ttctatttgg
     1921 tccaggggta gttatgcagt ccttaacagc gacggccttg gcatcaataa ttctgcgcca
     1981 ttaacaaggc ttcatatttc cagcgggcag gatgccggga tgagtaatac atctaacggg
     2041 tatattatgc ttggttcagg cacaagctct aatattattc ttgataacaa cgagatcatg
     2101 gcccgtaaca acggtgtaat agccccactt accttgcaga atgacggggg atcagtccgt
     2161 atcgggaatg tggcagcacc ggcagggtac ctttttgctg tgaatggaaa aatgataagc
     2221 gaagaattga aagtgcagtt atccggcaac tggcccgatt atgtctttaa aaaggattac
     2281 cagttaaaga gtttcgatga gttaagaaaa ttcatcgcag agaataatca tttgcccaat
     2341 atccccccgg cagcggaggt agaaaagaat ggactggaag tgggggatat gcaaaaaaag
     2401 atgatggaaa aaatagagga actgacattg tatgtattac aattggaaga gcaactgaag
     2461 gaaattaaaa agaaaatatc tgaccaataa aatattttta tgctgcggat aaattctttc
     2521 ctggctgttt tgtctttttc tgctttacaa cttgcgggcc agccacaatt tgagttgtca
     2581 cgaaatgtat acagggtccc ttattctaac gggataaccg ttactgttaa taatgaccat
     2641 tacacacatg atccgttggg aaggtatgac ttgaagggct ctggatttgg tggaagttgt
     2701 ggcgtcgcct acccaatcgt tgctgcggcc gaaggcatca tcaggcgtat cgttgataat
     2761 aacaatgtac atgggccaga ctgtagttct aattgtacgc agtataataa ttatgtctgg
     2821 atagaacatg caaatggaga gtggacaaaa tattcccata tgaaatacca aagtactacc
     2881 attgatgctg ggttatcggt gggagaccag gtttgtgccg gtactttttt gggctatgaa
     2941 tgtgatattg gccaagccac tggcccccat cttcattttg aggtcaggca tcccaatgat
     3001 acggcaaatg tgcagatatc tacttccggt ggatttatgt cagacgcaga tcacaggatc
     3061 cctgtcatca atacaatatc aaaacattac ctttctgatg gtgatagctg gacagcctca
     3121 ggttctacgg cttgtacatc aacagacatt gctttgatcc ttgcaacaac aataagtaat
     3181 aacggtatac gggcatatat ggcatccgga accattacaa cgtttgcagg catagggttc
     3241 acgtttcaaa atggttcaaa tggattattc caggcaggcg gtagcgttac tttatctccc
     3301 ggatttacgg catctgcagg ttcttatttt gaagcaaaga ttggtagttg cgccacaact
     3361 aatatggtag gagcatgtaa ttaattggac aacgctaatt attgaatatg atatggatga
     3421 aattaattca gcttatatgt tctttgtgtt aattgattat gattgcaaat gtcagcaagg
     3481 aaaagaatgg aaaccgaggt gatattgata tgccgggtat caaagcattg aagctgtgaa
     3541 aaagatagga cgactgatgc tattgttcag attgtaattc acacggtttt tgatgacaac
     3601 acccatgtat ttgaagccat gtatgcaggt gccaatggct acctgctcaa gaaatatgtt
     3661 tcagacaagc tgattcaatc tatacgggag gtgctggacg gggggtgccc cgatgagccc
     3721 ttccattgcc cggatggtga tctccaatat gcaaaaaggg attcccattg cagataatga
     3781 ttaccagctt accaaccggg agaaagaaat attgcaattg ctctccaggg gtaatagttt
     3841 taaaatgatc gctgttgatc tgtgcatcag cctggatact gtacgtacgc atatcaaacg
     3901 catttacgat aagctgcatg tacggtcgca aatagaagcc gtaagtaaag cgatcagtga
     3961 aaagctggta tagattttcc gttacttagt tattctgttg ttatttaatt gtggcttgta
     4021 tcacatcatc agggtattgt ttagggtgta aactgattgc atctttagaa aattaaattt
     4081 tctttaaaaa tttcctgatg agatataaaa tacctgtctt tataattttc tttttttcat
     4141 tggtgctcac acatgcacag aatgttggca taggtaccga acggccgctc agcaagctgc
     4201 aggtagatgg tagctttagt gcaagatctt tatattcctt cagcagtagc tcacccactg
     4261 cagggcaaac gtacagtatg attaatggaa acactgtaca aatcccggac accgacagtg
     4321 tagcccgcct ctacgatcac ggtggtatgg cagggaacta cttacccaac cttaccggaa
     4381 atacgcttat aaatttaaat ggcactattg acagctacat ggaagcagtg attgaatcag
     4441 taaaccttgg taccggcgac tccctcatta tatatgataa tagttatgct tcgcctggtg
     4501 aggtattgct ggcagtaggc aatggttata cggcaaccaa tctcagcctg actttctcct
     4561 ctaatgttgg atactgctca ttcaaaagca atgctgatgc ttccgtagga tcaggttttt
     4621 ccatcttatt tacccggaaa ttccctaagc tcacttttct tgatattgca aacattactc
     4681 aaaaagccat gacctatagt cccaatttgg ctgcattcag ggtaggcaaa atttcaaacg
     4741 ccagcatcgg atggtattct gtagcaatgg gtcgcaataa tacagcatct gggcaggttg
     4801 ctattgcatt gggagataga actgaagcaa ctaatggaag tgccactgct atggggagtc
     4861 aaacaaaggc atcagggcaa tatgccaccg ccatgggata ccaaacacag gcaaccgatg
     4921 tatattcgac tgccatgggc agagcatcat gggcaaccgg cagtgcagca actgctatgg
     4981 gagaacgtac aacggcatcc ggtacagcgg ctacatcaat gggatattat acagtcgcat
     5041 caggggaatc tgctacggcc ataggagatg gtgcccttgc atccggtgtc actgcaatgg
     5101 catcaggata ctatactgtt gcgtcgggca ggtatgccac tgcaatgggt gcagaaacgc
     5161 atgcaaattc ctatgcgtct ttagctatag gaaggttcaa tgatgcacct gcatcaagtg
     5221 ccaacagttg ggtaagtaca gaccgagcat ttgtaattgg cgacggcact tccgatgcag
     5281 cccgttcaag tgctttcatt atttacaaaa acggcaatgg ctggatgcag ggtgcactca
     5341 gccagtcaag tgatgcaaga ctcaaaaaga acattcataa actagaccag gcgctggaca
     5401 aaatcaaccg ccttaacggc tacaactatt actggaaaga cgaaggaaat atacccggcc
     5461 ttcaaagcgg gctgctggca caggaagtac agcaagagat gcccgaactg gtcactacca
     5521 acacagaagg tgaattagcg gtgaactata atggcatggt accttacctg cttgaagcca
     5581 ttaaaacgca gcagcaacag atacaaacac agcaagaaca aataagcctc ttgaaaaaag
     5641 atgttgaaca actaaagcaa actaaaaaga aaagtcggaa aaaagtttat cgttgaaagc
     5701 tgcagcggta aggtcccgcc tcaaatttgt taagtattat ttttccatca catcattgcg
     5761 gtattgttat ttatggttac agcaatgaat tttacatcta ttcaaaaacg ataattatga
     5821 aaaggctgat tttgttttcc tttcttttgt tttcaactgt tttactatat gcacaaaatg
     5881 tagctattaa taatgacggg agtcagccgc atgccagcgc agcgctggat attaaagcaa
     5941 cagataaagg actgctgttg ccgagggtgg caaacccggc cggtgcggtg agtaatgctg
     6001 cggaaggatt gctgctgtat aatactacta cacaacaacc caattttttt aatggaacaa
     6061 actggcaggg aatgggcggg gcattaagtg ccaggtttcc cagggccata gctttccggg
     6121 gcgatgtaaa ttccgctaat agccagacca atgctacgtg gacagtacct gcaaacatca
     6181 cccaggtgtg ggtggaaatg tgggcagccg gcgatgctgg ccaaatatta tctagtgctg
     6241 ttgcatcaga tatgtctgac cc
//
LOCUS       DAORTI010000169         6240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14769_length_6240_cov_6.138399, whole genome shotgun sequence.
ACCESSION   DAORTI010000169 DAORTI010000000
VERSION     DAORTI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14769_length_6240_cov_6.138399"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1857)
                     /locus_tag="PLO70_14970"
     CDS             complement(442..1857)
                     /locus_tag="PLO70_14970"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQZ75820.1"
                     /translation="MSDNYSFFGAIEKSFDKAAKFTKWDTGILDQIRQCNAVFQMRFP
                     LKRDDGTIEVIEAYRVQHSHHKTPCKGGIRFAAEVNQDEVMALAALMTYKCAIVNVPF
                     GGGKGGIKINPKKYSTFELEKITRRYTSELIKKKFIGPGTDVPAPDYGTGEREMSWIL
                     DTYTAMHLGEIDAAGCVTGKPITQGGVRGRREATGLGVFFGVREVCNMPDIMGKLGLS
                     VGVEGKNVVVQGLGNVGYHSAKFFQDAGAKIIALAEYEGAIWNKDGLDVDAVFNHRKT
                     TGSILNFPGATNFKESSEALEMECDILIPAALENVIDGDNAPRIKAKLIGEAANGPLT
                     PEADEIFSAKGIVVIPDMYLNAGGVTVSYFEWLKNLSHVRYGRLEKRFTENMNSHILG
                     QIEELSGKKVKENAREMIMHGPDEVDLVYSGLEETMINATHEIMNLWKDNPDIPDMRT
                     AAYVVAINKVATSYAELGIFP"
     gene            complement(1938..2153)
                     /locus_tag="PLO70_14975"
     CDS             complement(1938..2153)
                     /locus_tag="PLO70_14975"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75821.1"
                     /translation="MRNSRKIIMMLATVLTFLPIFAQDKKTEMADLMRENGRIYVVVA
                     VVLTILIGLILYVVRLDKKISRLEKEN"
     gene            complement(2150..2866)
                     /locus_tag="PLO70_14980"
     CDS             complement(2150..2866)
                     /locus_tag="PLO70_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQZ75822.1"
                     /translation="MTDTNNYMYKSWWKIASIALLIYTFIAGFLWEVPRLPLLQETIR
                     NLYFHVCMWFAMMILFTVSVVNAIRYLRTFNLKYDIYARHFAVVAIVFGLLGYLTGAI
                     WASYTWADPNKPAINSFSTIARDPKLIGAAIALLVYFAYLILRDSIQDMDKRARISAV
                     YNIFAYAILFPAIWILPRILPSLHPGNEDNPALDVKNDISGSMRIVFYPAVIGWTLLG
                     VWITTLKIRFTLLKEKKMMP"
     gene            complement(2981..3637)
                     /locus_tag="PLO70_14985"
     CDS             complement(2981..3637)
                     /locus_tag="PLO70_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQZ75823.1"
                     /translation="MSSLHHIITLFKKDLLLEVRQQYSFYGILLYIGATIFVLFMAIE
                     EPESKVWNGLFWVIQLFICINAVAKSFLQESKGRMLYFYSIASPTAFVLSKLLFNSLL
                     MLVMSVLSLLLFTLFLGNPMLKAGQFVGLVLLGGWSLSLVFSFLAAIAAKAQQNAAIM
                     AVLGFPIIIPQLILLMRLSNAAFAPLLTIPVSTVLLLVALDAMIILLAVILFPFLWKD
                     "
     gene            3719..4645
                     /locus_tag="PLO70_14990"
     CDS             3719..4645
                     /locus_tag="PLO70_14990"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HQZ75824.1"
                     /translation="MNKVKFSLLLVTAVAFSFIFCFSSCQSTRSATTSRMLKFNFEKG
                     KGYDYEMNINMNQEIMGSPTKLDISTYYSMDVQEDDGTVKTLQTKYDRFKINMDVMGM
                     QLDVDTDKPLPATGEEDMNPMSKVSKLFAAIKGQEFTMKVDAEGKILEVKGFKEMAES
                     IVSSMDIDDKGGKEESEKMKKQFDQQFNDKNIREQFERVLYIFPNKEVKVGDSWEKST
                     NMAGQLTGKMNSTYTVTDIEGDMVTLEEKTKIGSAEEGAELEGEQNGILVVDSRTGLV
                     VTADLVMNMTAKAGGIAIDINGKTKIKGKARQ"
     gene            complement(4790..5356)
                     /locus_tag="PLO70_14995"
     CDS             complement(4790..5356)
                     /locus_tag="PLO70_14995"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQZ75825.1"
                     /translation="MFIEPNTSGERRGSIEVICGSMFSGKTEELIRRLKRVKIANQKV
                     EIFKPAIDVRYDEVKIVSHDTNAIQSTPIDNSQKILLMAGDVDVIGIDEAQFFDEEIA
                     NVCDELAFRGIRVIVAGLDMDYLGKPFGQMPYIMAKADYVTKLHAICMKCGSIANYSY
                     RKIPNEDQVMLGATDVYEPRCRKCYHEK"
     gene            5455..6180
                     /locus_tag="PLO70_15000"
     CDS             5455..6180
                     /locus_tag="PLO70_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75826.1"
                     /translation="MERIEILINKLKEQFEQKADPALLLGTVQLLQFELTKLKSPGFM
                     GLGTAKVAVVLPAAANMATSTVGLPQYEKYAPKPVEKQIVVEAKETRETKETVLVDDG
                     SVSLVQKNGQLDMVFDPMSEIPTFSHQLKDREVNDTNGPAESLNDKLKHGKTELMEVL
                     KETPVKDLRKAIGINDRFLFIHELFRGDENMYERSIKTINNFHIHAEAEYWISRELKL
                     KLGWDGASTAVQHFDQLVKRRFS"
BASE COUNT         1920 a         1247 c         1264 g         1809 t
ORIGIN      
        1 aataccggtt attttaacag acgttactga attaaatgca tccaagccgc ccatggctgc
       61 tgtatgtttt tgtattattt catctgcggt ttgtgcctga gcagatataa ctgcaaaacc
      121 aagcagtgtc aaaaagaatt ttttcatgaa ttttttgatt attgaaaagc gaagatagga
      181 atttcatttt tgggtagtta ttgttacaga gtttgaaaag tagacctatt ttccgaatac
      241 tacattccct tatctgttgg gtataatttt acagcataat ccttcttgta ctgatcagac
      301 tttcatttct tttatttttc tcttttccga gtaagccgtc attcttgagg gttttaaatg
      361 tgtagagtaa caggagaata aaaaaatccc gtctgctttt tgcaaacggg attgtaaaat
      421 aagcaattgg tttgaggtct tttatgggaa aattcccagt tcagcgtagc tggtagcaac
      481 tttattgata gccacaacat aagccgctgt cctcatatcg ggtatatcag gattgtcttt
      541 ccataaattc attatctcat gggttgcatt aatcatggtt tcttccagtc cactgtaaac
      601 aaggtctact tcatccggtc catgcataat catttcccgt gcattttcct ttactttctt
      661 gccgctgagc tcttctattt gccccaaaat atggctattc atattttctg taaagcgttt
      721 ttctaaccgg ccataacgaa catgactcag gtttttcagc cactcgaaat atgaaacagt
      781 aacacctcct gcattcaggt acatatccgg tattacaaca atgccttttg cggaaaatat
      841 ttcatctgcc tctggtgtta gcgggccatt ggctgcttct ccgattaatt tagctttgat
      901 tctcggtgca ttatcaccat caattacatt ctccaaagct gcaggaatca atatatcgca
      961 ttccatctcc aatgcttcac ttgattcttt gaaattagtc gcaccaggaa agtttaaaat
     1021 ggaaccagtg gtcttgcggt gattgaatac ggcatctaca tccaggccat ctttgttcca
     1081 gatagcccct tcatactctg ccagtgcaat aatttttgct ccggcatcct ggaagaattt
     1141 tgcagagtga tatcccacat tgcctagacc ctgtaccact acattcttcc cttctacacc
     1201 aacagaaagt ccaagtttcc ccattatgtc cggcatattg catacttcac gaacaccaaa
     1261 gaaaactcca agtcctgttg cttcacgacg tccacgaact ccgccctgtg taataggttt
     1321 tccggtaacg caaccagcag catctatttc accgaggtgc atagctgtat aagtatcaag
     1381 tatccatgac atttcccttt cgccggttcc atagtcagga gccggaacat ctgttccagg
     1441 acctataaat tttttcttga tcagttctga tgtatacctg cgggtgattt tttccagttc
     1501 aaaagtagaa tactttttag ggttgatttt aatacctcct ttaccgccac caaatggaac
     1561 gttaacgata gcacatttat aagtcatcag cgcagcaagt gccattactt catcctggtt
     1621 tacttctgct gcaaaacgaa tacctccttt acagggagtt ttgtgatgag agtgctgtac
     1681 acggtaggct tcaattactt caatcgtccc atcatctctt ttcaggggaa agcgcatctg
     1741 gaatactgca ttacattgcc tgatctggtc aaggatacct gtatcccatt ttgtaaattt
     1801 ggccgcttta tcaaaacttt tttcgatagc gccaaaaaaa ctgtaattgt ctgacatgat
     1861 ttctttttta aatttttgca atcgttattg taggtaacaa acatgaagtg ttataaccat
     1921 catgtttcgc tagtaaatca gttttctttt tccagcctgc ttattttttt atccagtctt
     1981 accacataca atataagtcc tataagtata gtgagtacta ccgctactac tacgtagata
     2041 cgaccattct cccgcataag atcagccatt tctgtttttt tatcctgcgc aaaaatggga
     2101 aggaatgtca aaactgttgc cagcatcata attatttttc tgctatttct catggcatca
     2161 tctttttttc tttcaataat gtgaaacgga tttttaatgt ggttatccat actcccaaaa
     2221 gggtccagcc gataactgct ggataaaaaa caattctcat ggaaccgctt atgtcatttt
     2281 ttacatcaag ggccgggttg tcttcattgc ccgggtgcag gctgggtaaa attcttggca
     2341 aaatccagat agccggaaac aaaatggcat aagcgaatat gttataaaca gcgctgattc
     2401 tggctctttt gtccatgtcc tgaattgaat ccctcagtat caggtaagcg aaatacacta
     2461 acaatgctat agctgcacct atcaatttcg ggtcccttgc tatagtactg aaagaattga
     2521 tagccggttt atttggatcg gcccaggtat aagaggccca aatcgccccg gttaagtaac
     2581 ctaacaggcc aaaaacgatt gccaccacgg caaaatggcg ggcataaata tcatatttaa
     2641 ggttaaaagt tctcaggtaa cggatggcat taactacaga tactgtaaaa agtatcatca
     2701 tggcgaacca catacataca tggaaataaa gattgcggat tgtttcctgc agcaacggca
     2761 aacggggcac ctcccataaa aagccggcaa taaacgtata gataagcaat gcaatacttg
     2821 caatcttcca ccaggactta tacatatagt tattagtgtc agtcatcaga atagccaaag
     2881 aagattcatg gccgggtaaa gtgaggcaaa attaaggtga tgcaactaaa cgttcgtaaa
     2941 aattcttttc cgctaatatg tgtgaaaaac acagggcatt ttaatctttc cataaaaagg
     3001 ggaatagaat tactgccagt aatataatca tcgcatctaa tgcgaccagt aacaggacag
     3061 tgcttaccgg gatggtgagc agtggggcga aagcagcatt agaaagccgc attaacaaaa
     3121 taagttgggg gataataatt ggaaaaccaa gtacagccat gatcgctgcg ttttgctgcg
     3181 ctttggcggc aatagctgcc aaaaaactga aaacaagact gaggctccag ccacccaata
     3241 gaacaagccc tacaaactga cccgctttga gcatcgggtt gccaagaaaa agggtgaata
     3301 atagaaggct cagtacactc atcaccagca tcaaaagaga attgaaaagc agtttagata
     3361 aaacgaaagc cgttggactg gcgatagaat aaaaatatag catcctgcct ttgctttcct
     3421 gtagaaagct tttagcaacg gcgttaatgc atataaagag ttgaattacc cagaacagac
     3481 cattccatac tttactttcc ggttcttcaa tggccataaa taaaacgaaa atggttgcac
     3541 ctatatataa cagtattcca taaaaactgt attgctgtct tacttccagc aacaggtctt
     3601 ttttaaagag agtaattata tgatgcaggg aactgataaa tgtaggtttg ccggcaaaaa
     3661 tagtacttac aggtagcttc tctcttactt taatgttcta ttttgccaaa aaaaagcaat
     3721 gaataaagtg aaattttcac tactacttgt gactgcagtt gccttcagct tcatcttttg
     3781 cttcagttcc tgccagtcta cccggtctgc cactacctcc aggatgctga aatttaattt
     3841 tgagaaaggt aaaggctacg actacgagat gaatattaat atgaaccagg agataatggg
     3901 cagccccaca aaattagaca tcagcactta ttattctatg gatgtacagg aagatgacgg
     3961 cacagtaaag accttacaaa ctaaatatga caggttcaaa attaatatgg atgtgatggg
     4021 tatgcagtta gacgtggata ctgacaagcc tttaccagcc acaggtgaag aggatatgaa
     4081 ccctatgagt aaagtgagta agttatttgc tgcaattaaa gggcaagaat ttacaatgaa
     4141 ggtagatgca gaagggaaga tactggaagt aaaagggttt aaagaaatgg cagaatcaat
     4201 tgtttcctcg atggatattg atgataaggg aggaaaagag gaaagtgaaa aaatgaaaaa
     4261 gcaatttgat caacagttta acgacaagaa tatcagggag cagtttgaaa gggttttata
     4321 catttttcct aataaagaag tgaaagtggg ggatagctgg gaaaaaagta caaatatggc
     4381 tggtcagctg acaggaaaga tgaactcaac ttatacggta acagatatag agggtgatat
     4441 ggtaacactg gaagagaaaa ctaaaatcgg gtccgccgaa gaaggtgctg aactggaagg
     4501 ggaacaaaac ggtatccttg tcgttgatag tcggacagga ttggttgtta cagccgacct
     4561 tgttatgaat atgactgcaa aagcaggagg aatagcaata gatattaatg ggaaaacaaa
     4621 aataaaaggg aaagcaaggc agtaaactta aaaatggttt aaaaagaggc tatttcaaat
     4681 cccggtttga ttgtcatttt gatccgatcg caatcgggat accgggatta tggcaatcat
     4741 tgcgatagtt cccggtatgt tcaggctggc atacattctt gagacagcct tatttttcat
     4801 gataacattt tctgcaacgg ggttcataca catccgtagc gcccagcata acctggtctt
     4861 cgttggggat ctttcggtaa gaataattgg caatacttcc gcatttcata cagatagcat
     4921 gcagttttgt tacataatcg gcttttgcca ttatataagg catttgtccg aagggcttac
     4981 cgagataatc catgtccagc cccgcaacaa tgaccctgat accgcgaaaa gccagctcat
     5041 cgcagacatt ggcgatttcc tcatcaaaaa actgtgcttc atcaatacca ataacatcaa
     5101 catctccggc catcaacaat attttttgtg aattatcaat tggggtggac tgaatagcat
     5161 tggtatcatg cgatacaatt tttacttcat cataccgtac atcaattgcc ggtttgaaga
     5221 tctccacttt ctggttggca atctttactc ttttcaggcg acggataagc tcttctgttt
     5281 tgccactgaa catagagccg cagatcactt ctatacttcc acgccgctca ccactggtat
     5341 tcggttcaat aaacattgtt aagattttgt gtgactttat ggtgattgat tatattggca
     5401 aactgtttgt aactttaatg ggtctcaatt tttgctggcg tcaaatataa atgaatggaa
     5461 cgaatagaaa tcctgatcaa taaattaaaa gaacaatttg aacagaaagc agatccggcc
     5521 ttgttgcttg gaacagtcca gttgcttcaa tttgaattga cgaaacttaa gtcccccggt
     5581 ttcatgggtc tgggtacggc caaagtcgct gtggtacttc cggctgcagc caatatggca
     5641 acttcaacgg tcggattgcc ccagtatgag aaatatgccc caaaacctgt ggaaaaacaa
     5701 attgtggtgg aagcaaaaga gacccgggaa acaaaagaaa ccgttttggt ggatgatggg
     5761 tctgtttcat tagtacagaa aaacggccag ttagacatgg tgtttgaccc catgtcagaa
     5821 ataccaacct tttcacacca gttaaaagac agggaagtga atgatacaaa cgggccggca
     5881 gagtcgctta atgataaatt gaaacatggt aaaacagagt tgatggaagt gttaaaagaa
     5941 acacctgtga aagaccttcg caaagccata ggaattaatg atcgcttttt gttcattcat
     6001 gaactttttc ggggtgatga gaatatgtat gagcggagca taaaaaccat caataacttt
     6061 catatacatg ctgaggctga atactggatc agccgggaac tgaagttaaa actgggatgg
     6121 gatggtgcca gcactgctgt acagcatttc gatcagttgg ttaaaagacg gttctcctga
     6181 ataaaatgca aaatcctttt ggttgcacct ttatgtgatt gtacaaattc tccggccgca
//
LOCUS       DAORTI010000170         6221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14844_length_6221_cov_8.735485, whole genome shotgun sequence.
ACCESSION   DAORTI010000170 DAORTI010000000
VERSION     DAORTI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14844_length_6221_cov_8.735485"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLO70_15005"
     CDS             complement(<1..257)
                     /locus_tag="PLO70_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HQZ75827.1"
                     /translation="MCYDMSFFSNIKLISDYLNITSGETIQFEPTYHRVAQSYGKWPV
                     VINENGYKIKLFEWGLIADYMNTPEKIKEYRSSMANARSEK"
     gene            complement(242..763)
                     /locus_tag="PLO70_15010"
     CDS             complement(242..763)
                     /locus_tag="PLO70_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75828.1"
                     /translation="MLNPYTLLTPQILAALLRQPMYFVRQEYKRGIFVMDSEKTTSLL
                     LTHYARHETDKERAHRHMRLLMKDQYRFMYDSTNEEHFQKLKIAAEQPPGFRVYINLM
                     PKEWKASDGLKRKISSYMLYKLPWWQYSPADKLKVTLKERYGELYLALLWKGQQTEVH
                     LDEIEKFIPCATT"
     gene            complement(771..2051)
                     /locus_tag="PLO70_15015"
     CDS             complement(771..2051)
                     /locus_tag="PLO70_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HQZ75829.1"
                     /translation="MIAIVDCNSFYCSCERLFRPDLDDKPVVVLSNNDGCIISRTDEA
                     KKIGVGMAAPYYQNKEIIEKNNVAVFSSNYNLYGDLSMRVMDTLRSLVRKEQVEVYSV
                     DEAFLQLGMISENNLYKACTEIKETVEQWTGIKVSIGAGPTKVLSKVANRLSKKNKKE
                     SKGVMVLQSVHEIKDALERTYVDDIWGVGRQYAVKLHNLGIDNGWQLRNMPEEWARKN
                     MGGVVGTRLIKELRGVPCIPMKDPLEVKKMIATTRMFGKPVFELSEIKEAVATYVSRA
                     AEKLRRQYYAATSIQVFVVTNGYRKEKYEYNPQSRYQHSNFLVATADTAELIQYALPL
                     AEKLYEPGLKYLKAGVILGGLVPDNSIQANLFVHEEQNQKRFLMEAVDNINFSNRDDI
                     IKYAASGLKRNWKMRQELRSKRFTTRWNELYEIK"
     gene            complement(2048..2479)
                     /gene="umuD"
                     /locus_tag="PLO70_15020"
     CDS             complement(2048..2479)
                     /gene="umuD"
                     /locus_tag="PLO70_15020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HQZ75830.1"
                     /translation="MEGEEFYGAAYKGSKQFSQQQVKTANATGFGAAADDYMERGIDL
                     NEQLIKNKPATYFFRMKGDAMREAGIFDNDVLIVDRSVKLANGKIIVAVLNGELLVRR
                     FHKNFSSAFLIPENARYKSINLAEFSDFVVWGVVTYVIHQV"
     gene            2566..3180
                     /locus_tag="PLO70_15025"
     CDS             2566..3180
                     /locus_tag="PLO70_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQZ75831.1"
                     /translation="METIVNTIPGYRVYEYLLVLSPHEELWNRIMKVKENFAEGYKSD
                     HARWGGPQIALVNFSQYEMMEERIVNRLRTVAMGYPPFKVELKDFGSFPSHTLYINVT
                     SKLPVQNLVKKIRTDGQRLMKLNDDNKPLFSMEPHLTIARKLKPWQYEKGWLEYSNKH
                     FTGRFIADSMSLLKRPAGDRKYRLIHQFEFQNLPVTTKQGELFG"
     gene            3345..4454
                     /locus_tag="PLO70_15030"
     CDS             3345..4454
                     /locus_tag="PLO70_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQZ75832.1"
                     /translation="MSAKLKQDHFKVTAKQQEDGQQYIHGRGAQFNTKNRFLKNEITR
                     EHTESIDDWEESNVPTVYLEQESKTIVNKVESKDVGMGYSMNPYAGCEHGCIYCYARN
                     VHEYWGYSAGLDFERKIIVKKNAPQLLRKFLMHPKWDATPIMLSGNTDCYQPAEQKYR
                     LTRGLLEVCNEFNQPVGILTKNSWILKDKDVLQEMAKKKIVSAMVSITSFNEDLRRTM
                     EPRTTTAKQKLKVINELSTAGVRMGIMMGPIIPGLNEHEMQRIMKAAADNGATFTAYT
                     FIRLNGAIKLLFHDWLYKNFPDRADKVWHLIEQSHDGKVNDTRWGVRMRGEGNIAEMV
                     AQQYKKYGKLYRMNAEEWSLDTTIFRRPGEQGKLF"
     gene            complement(4446..4865)
                     /locus_tag="PLO70_15035"
     CDS             complement(4446..4865)
                     /locus_tag="PLO70_15035"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQZ75833.1"
                     /translation="MKLITFIGTGAFIVLSSFIMKKGNTNQSVSLYETKWSLKKIHTG
                     NNVPDMPANAFIRFDQEKKRAGGNGGCNSFGGSLVVMNDLINISTIFSTKMFCEETQQ
                     TEDAFFKQLEKVNRFEIKGKTLMLYRDKELLLEFVSE"
     gene            complement(4968..6212)
                     /locus_tag="PLO70_15040"
     CDS             complement(4968..6212)
                     /locus_tag="PLO70_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HQZ75834.1"
                     /translation="MNQRFYSLDVFRGATVCLMILVNNPGSWAHIYAPLEHAPWHGLT
                     PTDLVFPFFLFAVGNAMSFVMPRLEAAGDGVFWRKVLKRTAIIFLIGLFLNWWPFVTE
                     VNRLAEGTTELQKVTIFKGFTWLDYSKDKAGNIVETVKGVRVFGVLQRIALCYFFASV
                     IVYYLKARKAFLVGMIMLLLYWMLCYIGNPADPYSLKGWFGTDIDKTILGISHIYKGE
                     GIPFDPEGLMSTLSAIVQVIFGYLVGDYIQKKSKLLPVPDKPGETTGGMYPMLTGLFV
                     IGVALLVTGFCWDMVFPINKKIWTSSYTIYTTGLAIITIATMIYMIEIKNIRGGLARF
                     FDVFGKNALFVFALSAFLPKGLALIKLGNGVNPWNWLYKKVLIHTPGAKENGSLLYAL
                     CVITFMWAICYWMDKKKIYIKV"
BASE COUNT         1839 a         1293 c         1249 g         1840 t
ORIGIN      
        1 attttttcac ttcgggcatt ggccatacta ctcctgtatt cttttatttt ttccggagta
       61 ttcatataat ctgcaatcag gccccattca aataatttta ttttataccc gttctcgttt
      121 attaccaccg gccattttcc ataggattgc gctaccctgt gataagtggg ttcaaattga
      181 atagtttccc cgctagtgat gttcaggtaa tcactaatca atttgatatt actgaagaaa
      241 ctcatgtcgt agcacatggg ataaattttt ctatttcatc caaatgtact tctgtttgct
      301 ggcctttcca caagagggcc aggtacaatt ctccgtacct ctcttttaag gttactttca
      361 atttatcagc tggtgagtat tgccaccagg gcagtttata aagcatatag ctgctgatct
      421 ttcgttttaa tccatctgat gctttccatt cttttggcat caggttaata tagaccctga
      481 aaccgggtgg ctgctcagct gcgattttta atttttgaaa atgctcttca tttgtactgt
      541 cgtacataaa acgatactga tctttcatca ataggcgcat atgacggtgt gcccgttctt
      601 tatctgtttc atgccgggca tagtgtgtca gtaatagcga agttgttttt tcgctatcca
      661 ttacaaagat tcctctctta tattcctggc ggacaaaata catgggttgc ctgagcaggg
      721 cagctaaaat ttgcggcgtt aataacgtat aggggtttag cattttgcaa ctattttatt
      781 tcatacaact cgttccaccg ggtagtaaat cttttacttc tgagctcttg cctcattttc
      841 cagtttcgtt ttaatccgga tgctgcatat ttaattatat catctctgtt gctgaaattg
      901 atattatcaa ctgcttccat caggaaacgc ttttgattct gctcttcatg cacgaataga
      961 ttggcttgta tagaattatc aggaaccagt cctcctaata taacaccggc tttcaggtat
     1021 tttaaacccg gctcatatag tttttctgcc aggggcaaag cgtactgaat taattcagct
     1081 gtatcggctg ttgctaccag gaaatttgag tgttgatatc ttgattgggg attatactca
     1141 tacttctctt tccggtaccc atttgtaact acaaatacct gtatagacgt tgctgcataa
     1201 tattgacggc gtaatttttc agcagccctg gaaacataag ttgctaccgc ttcttttatt
     1261 tcgctcagct caaaaacagg tttaccaaac attcttgtag tggcaatcat ttttttaact
     1321 tccagcgggt ccttcatcgg gatacaggga acaccccgga gttccttaat cagtcttgtt
     1381 cctaccaccc cacccatgtt tttccgggcc cattcctccg gcatgttgcg caattgccac
     1441 ccattgtcaa tacccaggtt atgaagtttt acggcatatt gacgtcccac tccccatata
     1501 tcatcaacat aagtccgttc taatgcatct tttatttcat gcacagattg taaaaccatt
     1561 acacccttcg attctttttt attttttttt gatagccggt ttgccacttt agataaaact
     1621 ttagttggcc cggccccgat tgatacttta ataccggtcc attgttctac tgtttctttg
     1681 atttctgtac aggctttata taaattattt tctgaaatca tacctaattg taagaatgcc
     1741 tcatcaaccg aatagacctc tacctgttct tttctgacaa gagaccggag tgtgtccatc
     1801 acccgcatac tcagatcccc atacaggtta tagttactgg agaaaacggc gacattattt
     1861 ttctctatga tttctttatt ctgataatag ggagctgcca tgcctactcc aatttttttg
     1921 gcttcgtctg ttcttgaaat aatacaacca tcattgttgc ttaatacaac aaccggtttg
     1981 tcatcaaggt cgggcctgaa aagccgttcg caagagcaat aaaaactatt acaatccact
     2041 attgctatca tacctggtgg attacatatg taacaactcc ccacactaca aaatcgctga
     2101 attctgcaag gttaatagat ttataccggg cattctccgg aattaaaaat gccgatgaaa
     2161 aatttttatg aaaccgcctc accagcaact caccattcaa tacggccaca ataatttttc
     2221 catttgccag tttcaccgac cggtcaacga tcaatacatc gttatcaaag atgccggcct
     2281 cccgcatcgc atcacctttc atacggaaaa aataagtggc cggtttattt ttgatcagtt
     2341 gttcattcag gtcaatgccc cgttccatat aatcatcggc tgcagcacca aagccagtgg
     2401 cgtttgccgt cttcacctgt tgctgcgaaa attgcttcga gcctttatag gccgccccat
     2461 aaaattcttc cccttccata aaataatttt tgtaaagcta aataatttag taaattagtg
     2521 ctaagaaaat gggtaaatat tttttgatct ctaaatggaa gggttatgga aactattgtc
     2581 aatactattc ccggttaccg ggtttatgag tatttgctgg tactgagtcc gcatgaagaa
     2641 ttgtggaacc gcatcatgaa agtcaaagaa aattttgcag aagggtataa gtctgaccat
     2701 gcaagatggg gtggtccgca aatagcgctg gtgaatttct cacagtatga aatgatggaa
     2761 gaacgaattg tcaaccggct gcggacagta gcaatgggat atccgccatt taaagtggag
     2821 ttgaaagact ttggcagctt cccttctcat accctatata ttaatgtaac cagtaagctg
     2881 ccggtacaaa acctggttaa aaagatccgt actgacgggc aacggctgat gaagctgaat
     2941 gacgataata aacctctttt tagtatggaa ccacacttga cgattgccag gaagttaaaa
     3001 ccctggcaat atgaaaaagg ttggttggaa tacagtaata aacatttcac cgggcgattc
     3061 atcgccgata gcatgagctt gctaaaacga ccagccgggg acaggaaata tcgtcttatt
     3121 catcagtttg aattccaaaa ccttccggtt acaacgaaac aaggcgaatt atttggataa
     3181 aaaatccgta ctcaataaca gtaaccagtg ggatggggga tgtgagtaag gagaataaag
     3241 agtaagcttt tgtgagtgtg attatgaaga gataactctt tgttctcctt ttttattgcg
     3301 ggtatgtgac gaagtaatct tagaatcaaa accaaccata gtgtatgtca gcaaaattga
     3361 agcaggatca ttttaaagtg acggcaaagc agcaggaaga tggacaacaa tatatacatg
     3421 gcaggggtgc ccagtttaat actaaaaacc ggttcctgaa aaatgaaatt accagggagc
     3481 ataccgaaag cattgatgac tgggaagaaa gtaatgtacc aactgtttat ttagagcagg
     3541 aatcaaaaac catcgttaat aaagtagaaa gtaaagatgt aggcatgggg tatagtatga
     3601 atccttatgc gggttgtgaa catggatgca tctattgcta tgcccgtaat gtacatgagt
     3661 attggggtta cagtgccggg cttgactttg agcgaaaaat tatagtgaag aaaaacgcac
     3721 cgcaactgct gcgtaaattt ttaatgcatc cgaaatggga tgccactccc attatgttga
     3781 gcgggaatac ggactgttac cagccagcag aacagaaata ccggttgaca agaggtttgc
     3841 tggaagtctg taatgaattc aatcagccgg tgggaatact gaccaagaac tcctggatat
     3901 taaaggataa ggatgtgtta caggaaatgg ctaaaaagaa aattgtttct gccatggttt
     3961 ctatcacttc ttttaacgaa gaccttcgcc gcaccatgga gccacgaact actaccgcca
     4021 aacaaaaatt aaaagtgatc aatgaattaa gtacagccgg tgtacgtatg ggaattatga
     4081 tgggcccgat tatacccgga ttgaatgaac atgagatgca acgtatcatg aaagcggcag
     4141 ctgataacgg tgcaacattt actgcctata catttatacg gctgaatggg gctatcaaat
     4201 tattatttca tgactggttg tataaaaatt ttccggacag ggccgataag gtatggcacc
     4261 tgatagaaca aagccatgat ggtaaagtga atgatacccg ttggggtgtt cgtatgcggg
     4321 gagaaggaaa tattgctgag atggtggccc agcaatataa aaagtatgga aagctgtaca
     4381 ggatgaatgc tgaggagtgg agtttggata ctactatttt ccgcagaccc ggtgagcagg
     4441 gaaagttatt ctgatacaaa ctccagtaat agttccttat cccggtacaa catcaatgtt
     4501 ttacccttaa tctcaaatct attcactttt tccagttgtt tgaagaaagc atcttcagtt
     4561 tgctgtgttt cttcacaaaa cattttggtg gaaaagatag ttgagatgtt gatcaaatca
     4621 ttcataacaa ccagggaacc accgaacgag ttgcaacctc cgttaccgcc ggcccttttc
     4681 ttctcctggt caaatctgat aaaggcattt gctggcatat ccggtacatt attgccggta
     4741 tgaattttct ttaaggacca tttggtttca tacagggata cggattgatt tgtatttcct
     4801 tttttcatga tgaaggatga tagtacaata aaagctcctg tgccgataaa ggtgattagt
     4861 ttcatgtgat tatttttttg acaccaaaca gatgcaaaag gaaagccgtt tgcacagcaa
     4921 acggctaagc taaaatgata aaatcctgtt aacaggttga gtaggatcta gaccttaata
     4981 tatattttct ttttatccat ccaatagcag atggcccaca tgaaagtgat aacgcacaac
     5041 gcatacaata aggagccatt ctctttggca cccggggtat gaatgaggac tttcttatac
     5101 agccagttcc agggattaac accattgcca agtttaatca aggctaatcc tttcggaaga
     5161 aatgcactca aggcaaaaac aaacaaagca tttttgccaa acacatcaaa gaatcgggca
     5221 agcccgccac gaatgttctt tatctcaatc atgtaaatca tggtggcaat ggtgatgatg
     5281 gcaagacctg tcgtataaat ggtataggag ctggtccata tttttttatt gatcgggaaa
     5341 accatatccc agcaaaagcc ggtaaccagc aatgccacac caattacaaa aagtcccgtc
     5401 agcatcgggt acatgccacc tgtagtctct ccgggtttat caggtacagg aagcagttta
     5461 cttttctttt gtatgtaatc acctaccagg taaccaaata ttacctgtac aatggcagac
     5521 aatgtactca tcaaaccttc cggatcaaaa ggaatgcctt cacctttata aatatgtgag
     5581 ataccaagaa tcgttttgtc aatatcagtt ccaaaccagc ctttcagact ataaggatct
     5641 gcggggttgc caatataaca aagcatccag tatagcagca gcatgatcat gcctactaaa
     5701 aatgcttttc gtgccttcag gtaataaaca ataacagatg caaaaaaata gcacaaagcg
     5761 atacgctgta atacgccgaa tacccgtacc cctttaactg tttcaacgat attgccagct
     5821 ttgtcctttg aataatcgag ccaggtaaaa cctttgaaga tggtcacttt ctgcagttct
     5881 gtggttcctt ctgcgagacg gttcacttcg gttacaaagg gccaccaatt gagaaataaa
     5941 ccaatcagga aaataatagc tgttctcttc agcacttttc tccagaaaac tccgtcacct
     6001 gcggcttcca gccggggcat cacaaatgac atggcgtttc cgacggcaaa taagaagaaa
     6061 gggaatacga gatcagttgg tgttaaccca tgccagggtg catgttcaag tggtgcgtaa
     6121 atatgtgccc aggaaccggg attgtttact aaaatcatga ggcaaacagt agcaccccga
     6181 aaaacatcca gggaataaaa gcgttgattc atcctgattt a
//
LOCUS       DAORTI010000171         6200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_14922_length_6200_cov_7.673068, whole genome shotgun sequence.
ACCESSION   DAORTI010000171 DAORTI010000000
VERSION     DAORTI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_6200_cov_7.673068"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="lnt"
                     /locus_tag="PLO70_15045"
     CDS             <1..1088
                     /gene="lnt"
                     /locus_tag="PLO70_15045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQZ75835.1"
                     /translation="EGTKRYKNLVAGILILIAPVAFSLLGFTPTSSSKLLPATKSNIV
                     VVQPNIDPYEKIAAGTFEAQLQKLITISEKEIDNNTTLVVWPETALYMGNGIDEQNMR
                     ENFFLNPLWSFLKRHPNTSLLTGVESFRIFDTKTKYSREFAGQYFEAYNGAVLLDSNG
                     ASSFYHKSMLVPGVETLPWFLRFIDKWFEKFGGTTAGYAKQNERAVLTEKNGYKIAPS
                     ICYESIYGEFMSKYVRNGANLVCIVTNDGWWKKTPGHKQHMNYARLRAIETRTWVARS
                     ANTGISCFIDPYGNVYNPQPYNTTAAIKLSIPVNAASKTFFVRYGDILSRVMAGLSIL
                     LLVYTISLKFRKVVFKKKFPALQTDHE"
     gene            1081..1872
                     /locus_tag="PLO70_15050"
     CDS             1081..1872
                     /locus_tag="PLO70_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ75836.1"
                     /translation="MNKEIQITGKKIFYRVTGEGNPVMLVHGFGEDGTVWNNQVDFLK
                     DKFRIIIPDLPGSGKSELIENSSLEDMAEVLHQIIHEEDIDHCVMIGHSMGGYITLAF
                     AEMYWNHLTAFGLFHSSAYADSKEKITTRQKGIEFINEHGAFAFLKTATPNLFSPLTK
                     AENPGLIDKQINSLDNFSPAALVSYYEAMISRPDRTAILLQATVPVLFIIGKYDNAVP
                     PEDSLQQCHLPEKSYIHMLNRSGHMGMLEEPQQSNQILEKFLLDI"
     gene            1897..4515
                     /locus_tag="PLO70_15055"
     CDS             1897..4515
                     /locus_tag="PLO70_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ75837.1"
                     /translation="MKRFLLAITIFLALSSTSFAAHIKGGYFSYEYLGPGNGTNLRYR
                     VTLTVYMICNPSTAQVSNPINFSIFNAGNNQFIQNVSVSLTNQYNLNKVADEPCISGD
                     QQGCYYTVVVYDLPAVELPSTPDGYIIAYQRCCRIAGINNVSGSGSVGNTFAINIPGT
                     AVGQNAQTNSSPSFLVNDTAVVCGNSFFQYSFQASDINGDSLSYEFCNAYLGGDAGAN
                     AAPVTAANPPYVSVPYQAPFSGTQPMGSGITINPRTGIISGIAPSLIGEYVVCVCVNE
                     YRNGVLIGKTRKELHIRVGDCVPIEATLNPQYITCDGFTMSFANNTPSSDITSYFWDF
                     GVLAALDDTANVNAPTYTYADTGTYTIKLVTNRNQQCSDSTTAVVKVYPGFFPGFIAT
                     GSCFQNPYQFTDTTNTRYGTVNSWTWNFGDLATLADTSRIQNPQWTYPNPGTRTVSLI
                     VTNSKGCIDTAQVNITVLDKPLITLGFRDTLICRNDAVQLNASGTGNFNWTPLINITD
                     PSTATPTVSPTSTTWYYVNLNDNGCVNRDSVRVRVVNQVTLQAINDTTICQGDAIQLN
                     ATSDGLAFNWTPVANLNNPNIINPVAVTNNTTTYNVTATIGGCTATDQVVVTTVPYPV
                     ADAGTDPTICYNTSAQLNGNIVGSSFTWTPASYLNNPNILNPVSSPPRTTQYILSAFD
                     TQGCPKPGRDTVVVTVLPRVRAFAGRDTIVIVGQPLQFNGSGGVNYVWSPSTGLSSTT
                     IANPIGIYNSSIDSVRYKLVVTDQLGCADSAFVMVKVFKTVPYVFVPTGFTPNNDGLN
                     DQIRPIAVGIQKINYFSIYNRWGQLVFTTTTNGQGWDGKIGGREQNTGVFVWMVSAID
                     YLGRPFFQKGTVTLIR"
     gene            4641..5408
                     /locus_tag="PLO70_15060"
     CDS             4641..5408
                     /locus_tag="PLO70_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ75838.1"
                     /translation="MPKIVLITGATSGFGKSCAYKFATQGYDLILNGRRADRLQKLAD
                     EIENKYNVAVLQLPFDVREERAVFGSISSIPEEWKAIDVLVNNAGLALGRDYFEEADI
                     SDWNTMIDTNVKGFLYVAKAVSQIMAVRKKGHIINMGSIAGKEVYEKGNVYCATKFAV
                     DAISQGMRIDLLRHNIKVTSINPGAAETEFSLVRFKGDESTAKQIYDGLTPLTGDDVA
                     EVIYYCASLPPHVCINDLSITCLQQANTIYFNRKPNN"
     gene            5615..6118
                     /locus_tag="PLO70_15065"
     CDS             5615..6118
                     /locus_tag="PLO70_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75839.1"
                     /translation="MNKTYLIVAAFIFLHSTSFSQDVIRLQSCSNDMMSKQVDSLKNL
                     YGNDGYILLKEASINMESEFEMPVIVPLTQGSWYQFVFIGDYSSRLYEVRMYDWQERQ
                     VIYRQKRWGEIDGNVIVYSYVPQFSEFHMMKPVQVNKQKKKNLCGYVMLFKRSGSETG
                     SPVPQNP"
BASE COUNT         1871 a         1311 c         1265 g         1753 t
ORIGIN      
        1 gggagggcac aaagagatat aaaaatcttg tagccggcat tcttatcctg attgctccgg
       61 ttgctttttc attacttggg tttacaccaa ccagttccag taaattatta cctgcaacta
      121 aatctaacat tgtggtcgtt cagcccaaca tcgaccccta tgaaaaaatt gctgccggta
      181 cttttgaggc acaattacaa aaactgatca ccatcagtga aaaagaaatt gacaacaata
      241 cgactttggt agtttggccg gaaacagccc tgtacatggg aaatggcatt gatgaacaaa
      301 atatgcggga aaattttttc ctgaacccat tgtggtcgtt tctgaaaaga catcctaata
      361 cctctttact tacaggggtg gaaagcttca ggatatttga tacaaaaaca aaatattccc
      421 gtgaatttgc cggtcagtat tttgaagcct ataatggcgc tgtactactt gacagcaacg
      481 gcgcttcttc cttttatcat aaatctatgc tggtacccgg tgtagaaaca ttaccctggt
      541 ttttacgttt catagataaa tggtttgaaa aatttggtgg taccacagca ggctatgcaa
      601 aacagaatga aagagcggtg cttactgaaa agaacggtta taagatcgcc ccttctattt
      661 gttacgaaag catctatggt gagttcatga gtaagtatgt tcgtaacggg gctaacctgg
      721 tctgtattgt tactaatgat ggctggtgga agaaaacgcc ggggcataaa cagcatatga
      781 actatgcaag attacgggcc atagaaaccc ggacatgggt agcccgcagt gccaataccg
      841 gtatcagttg ttttattgat ccttacggga atgtctataa tccgcaacct tataatacaa
      901 ctgccgctat taagctttcc atacccgtaa atgctgcatc aaaaaccttt tttgtccgat
      961 atggcgatat actttccagg gtaatggccg gactcagtat tttattactg gtatatacca
     1021 taagtctgaa attccgtaaa gtcgttttca aaaagaaatt tcccgcactt caaactgacc
     1081 atgaataaag aaatccaaat aacaggtaag aaaatatttt accgtgtcac cggagaaggc
     1141 aacccggtca tgctggtgca tggctttggt gaagatggaa ccgtgtggaa taaccaggtt
     1201 gattttttaa aagataaatt caggataatc atacccgatc tgcccggttc cgggaaatct
     1261 gaattaattg aaaacagcag cctggaagac atggcggaag tccttcatca aattattcat
     1321 gaagaagata ttgatcactg tgtgatgata ggccatagta tgggcggata tattaccctt
     1381 gcatttgcag aaatgtactg gaatcatctt actgcatttg gtcttttcca ttcctcggct
     1441 tacgctgaca gtaaagagaa aataaccacc cgtcaaaaag gaattgaatt tattaatgag
     1501 cacggggctt ttgctttttt aaaaaccgcc acccccaacc ttttttcgcc cctgacaaaa
     1561 gctgaaaacc cggggttaat tgataaacag ataaacagtt tggacaactt ttcacccgct
     1621 gcactcgttt cttactatga agctatgata agccgacctg accggacagc tattttgttg
     1681 caagctactg tgcctgtgct ctttatcatc ggcaaatacg acaatgccgt accacccgaa
     1741 gacagcctgc agcaatgcca cctgcccgaa aaatcgtaca ttcatatgct aaaccgatcc
     1801 ggccacatgg gaatgctgga agaaccacag cagagcaacc agattctgga gaaatttttg
     1861 ctggatattt aaaattcaat ttcgaccaac gtatcaatga aacgattttt acttgcaata
     1921 acaatttttc ttgcattgtc ttcaacctct tttgcagcac atataaaagg aggttatttt
     1981 tcctacgaat atttgggacc gggcaatggt accaacctgc ggtaccgggt tacactcact
     2041 gtgtatatga tctgtaatcc atcaacggca caggtttcca atcccattaa tttcagcata
     2101 tttaatgcag gcaataacca atttattcaa aatgtaagtg tatctctcac caatcagtac
     2161 aatttgaata aagtagctga tgaaccctgt atttcaggag atcaacaggg ctgttattat
     2221 acagtggtgg tttatgatct gccagcagtg gaattacctt ccacacccga tggctatatt
     2281 attgcttatc aacgctgctg cagaattgca ggaattaata atgtatcagg cagcggcagt
     2341 gtaggtaata cttttgctat caatataccc ggaacagcag ttggccaaaa cgcacagacg
     2401 aacagcagcc catctttttt agttaatgat acagcagtag tctgcgggaa cagttttttc
     2461 caatattcat tccaggcttc agatataaat ggcgattctt tatcctatga gttctgcaat
     2521 gcttacctgg ggggtgatgc aggagccaat gcagcacctg ttactgctgc aaatccgccg
     2581 tatgtctctg ttccatacca ggctcctttt agcggaacgc agcctatggg atccggaatt
     2641 actataaatc caagaactgg tatcatcagt ggcattgcgc cttcgcttat tggagaatat
     2701 gttgtttgcg tatgtgtaaa cgaatacaga aatggcgtat tgatcggtaa gaccagaaaa
     2761 gaactccaca taagggttgg agattgtgta cccattgaag ccacgttaaa tccccaatat
     2821 attacctgcg atggctttac catgtcattc gccaataata cacccagttc ggatatcacc
     2881 agttattttt gggattttgg tgtgctggct gctttagatg acactgctaa tgttaacgct
     2941 cctacttaca cttatgcaga tacaggaacg tataccatca aactggttac aaaccgtaat
     3001 caacaatgtt ccgactcaac tactgctgtt gtaaaagtat atccgggctt cttcccgggc
     3061 tttatcgcca caggaagttg ttttcaaaat ccctatcaat ttacagatac aaccaatact
     3121 cgttacggta cagtgaatag ctggacctgg aattttggtg atttagcaac actggctgac
     3181 acgtccagga ttcaaaaccc gcaatggacc tatcccaacc cgggcaccag gactgtgtcc
     3241 cttattgtaa ccaacagcaa aggctgtatt gatactgcgc aggtgaatat tactgtattg
     3301 gataaaccac tcattacact gggtttccgt gatacgctga tctgccgtaa cgatgccgta
     3361 cagttaaatg caagtggtac aggaaatttc aattggactc cgctgataaa tattactgat
     3421 ccgtctactg ccactcctac tgtgtcacca acaagtacca cctggtatta tgtaaattta
     3481 aatgacaacg gctgtgtaaa cagggactcg gttagagtac gggtggtaaa ccaggtaaca
     3541 ctacaggcta ttaatgacac aaccatctgc cagggtgatg ctattcagct gaatgctact
     3601 tctgatggac ttgcttttaa ctggacaccg gtagccaatc taaataatcc caacattatc
     3661 aacccggttg ctgttaccaa taatactaca acttataatg tgactgctac cataggagga
     3721 tgtactgcca cagaccaggt agtggttact actgttccct accctgttgc tgatgccgga
     3781 acagatccaa caatctgtta taatacctct gcacagttga atggaaacat agtgggcagt
     3841 tctttcacct ggacaccagc atcctatttg aataacccga atattcttaa tccagtctct
     3901 tctccaccac gaaccacaca atacatacta tctgcatttg acacacaggg gtgcccaaaa
     3961 ccgggccgtg atacagtggt agtaactgta ttaccaagag taagggcttt tgcaggcagg
     4021 gataccattg ttattgtagg ccagccttta caattcaatg gaagtggcgg tgttaactat
     4081 gtatggagtc cgtccacagg ccttagcagt acaacaattg caaatcccat cggcatttat
     4141 aattcttcaa ttgatagtgt acgatataaa ctggtagtga ccgaccagct tggttgtgca
     4201 gattcagcat ttgtgatggt aaaagtattc aagacagttc cctatgtatt tgtccccacc
     4261 gggtttacgc ccaacaatga cggccttaat gatcagataa gaccaattgc cgtaggcatt
     4321 caaaaaataa actacttcag tatttacaac cgctgggggc aactggtgtt caccaccacc
     4381 actaatggcc agggctggga tggtaaaatc ggaggcaggg aacaaaacac aggtgtgttt
     4441 gtatggatgg tcagtgctat tgactattta ggcaggccct ttttccagaa gggaacagtg
     4501 acattaataa ggtgatcccc taatatttat tggcaactca ggtaataaaa tgatttcttg
     4561 ttttaaattg ctgactgaat cggttacagc caattattaa agaatggcta cgcactattt
     4621 accatctact attcaccact atgccaaaaa ttgttcttat caccggtgcc acttcaggtt
     4681 ttggaaaatc ctgtgcttat aagtttgcta cccaaggtta tgatcttatt cttaacggca
     4741 gaagagctga ccggcttcaa aaactggccg atgaaattga aaataaatac aatgtagctg
     4801 tactacagtt accctttgat gtgagggaag aaagagctgt attcggatca atcagtagca
     4861 taccggaaga atggaaggct attgatgtgc tggtcaataa tgcaggactg gcactgggcc
     4921 gtgattattt tgaggaagct gatatcagcg actggaatac aatgatcgat acaaatgtga
     4981 aaggcttttt atatgtcgcc aaagcagttt cacaaataat ggcagttcgt aaaaaaggtc
     5041 acatcatcaa tatgggttct atcgccggta aagaagtata tgaaaaagga aatgtgtatt
     5101 gcgctaccaa gtttgcagtg gatgcaatca gccagggaat gcggattgat ctgctccgtc
     5161 ataatatcaa agtaacttct atcaatcccg gggcagcaga aactgaattt tccctggtcc
     5221 ggtttaaagg agatgaaagc acggccaaac aaatatatga tggccttacc ccgctaacag
     5281 gtgatgacgt ggcagaagta atctattact gcgcctctct tccaccccat gtttgcataa
     5341 atgacctttc catcacctgt cttcagcagg cgaataccat ctattttaac agaaaaccca
     5401 ataattgaca gctatcaata ttattatgac aattcatatt aatctggtat cttagttgtc
     5461 ctccgcactt tatgttatcc gtttgccccg taaattgccc gtatattccc ttcgaaaatg
     5521 tacctgaata acaaactttc cgctataaaa tttaaaagcg ggaaaagcat caactatatt
     5581 tatgcagtat ttccaataca tgataaagcc tgttatgaac aaaacgtacc ttattgttgc
     5641 tgcattcatt tttctccaca gtaccagctt ttcacaagat gtaatccgct tgcaatcatg
     5701 ttccaatgat atgatgagta aacaggtcga cagcctgaaa aatctatatg gcaatgacgg
     5761 atacatttta ctcaaagaag cttctatcaa tatggaaagt gagtttgaga tgccggtgat
     5821 agttccgcta acacagggca gctggtatca gtttgttttt atcggtgatt attcttcccg
     5881 cctgtatgaa gtaagaatgt atgactggca ggaaagacag gttatctacc gccagaaaag
     5941 atggggagaa atagacggaa atgtaattgt ttattcatat gtaccccagt tctctgaatt
     6001 tcacatgatg aaacctgtac aggtaaataa acaaaagaag aagaacttat gtggctatgt
     6061 catgctgttt aaacggtctg gttctgaaac cggatctccg gttccgcaaa atccctgatt
     6121 ataaaaatat aaaaaacaaa acccatcttc aaaagagatg ggttttgttt tttagacctg
     6181 ataatttctg atcaagttca
//
LOCUS       DAORTI010000172         6058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_15496_length_6058_cov_7.993337, whole genome shotgun sequence.
ACCESSION   DAORTI010000172 DAORTI010000000
VERSION     DAORTI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15496_length_6058_cov_7.993337"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLO70_15070"
     CDS             <1..463
                     /locus_tag="PLO70_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HQZ75840.1"
                     /translation="LLAALVIIQFIHPKKNKAEGPQPNYIGNSFTVPVDVKPILDKAC
                     NDCHSNNSNYPWYANFQPIHWWLEKHIKDGKKELNFDEYTNRSLRYQYHKMEETIEMV
                     KEGEMPLNSYTWTHKDAKLTKEEKIKITGWAESVMDTMKAKFPIDSLIRKK"
     gene            491..985
                     /locus_tag="PLO70_15075"
     CDS             491..985
                     /locus_tag="PLO70_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75841.1"
                     /translation="MRTKAKQISGIFLTLLGLVPLLFILLITLKKHDIRHRMKEKIGQ
                     QQLQTIVLSEAEVIWMDKHEIWVNNSMFDIHTKKLDTGIYTFTGLYDEEETMLVEKER
                     TAAGENNDQTKLLAQLFKCLPVFCNQDTDIVDLNFQYNSYNQIVHPNPIYQFREILTP
                     PPKV"
     gene            1032..3239
                     /locus_tag="PLO70_15080"
     CDS             1032..3239
                     /locus_tag="PLO70_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75842.1"
                     /translation="MSNKYVLILLCSFCMAVNGYTQEDTTTEKTLGEVIVSVNKWEQK
                     LNELPNKIVKVNRYDILKNNPQTAADLLGQTGTVFIQKSQLGGGSPMIRGFATNRVLL
                     VIDGVRMNNAIYRSGNLQNVISIDALSTETAEVIFGPGSLIYGSDAIGGVMDFHTLDA
                     RFSKDKKIVVKGSVLGRYSTANKENTLHADINLGWKKWSLLSSLSYSKFDDLKMGKNG
                     GQDSYLRPEYVERINNKDSIVKNGDPRIQRFSGYDQLNFLQKIRFRPNEHWDLLYSFT
                     HAKTGVAPRYDRLIQYRNGALRFAEWYYGPMLWNMHTLQILHSKKTSIYEDARLTVAY
                     QDYKESRIDRSKANINRNRQVESVDAISANWDATKNTGKGQLFYGLEYVHNKVGSFGE
                     RTNISTNVVTPMVSRYPDGSTWSTTGIYGSYKINLHTKVTLTTGLRYSYNTLDATFDT
                     TFIKFPYKNAKLRDGALTGNAGLVFRPAEGWQLNGNISTGYRMPNVDDIGKLFESVPG
                     NISVPNPDLTPEYAWNFEMGIIKTIPNKFRIELNGFYTILKNAIVRRPTTFNGSDSIL
                     FNGILSQVEALQNVAKATVSGFQASAEFYFTKSVSLQTHANWISGKETDDINDEQVPL
                     RHAPPFYGSTSIRYRLKKIFVEASAQYNSEIENVKLAPSEQVKTDIYAKDADGKPYSP
                     GWHTVNLKATYQLSKNLLVTTGWENITNQRYRPYSSGIVAAGSNFIISLRVSL"
     gene            3286..3858
                     /locus_tag="PLO70_15085"
     CDS             3286..3858
                     /locus_tag="PLO70_15085"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQZ75843.1"
                     /translation="MSSTKQQAKWIRRFRWLHRKIAIFLFIFFSLIAITGLLLGIKKQ
                     TGLLAPTQKGISADLSTWLPIDSLQKIAIKYLQDSVSVSLNSEIDRIDIRPEKGIAKF
                     IFKDHFNGLQLDGSTGRLLSIEKRRSDFIENLHDGSILDKLLGTGGDQVKVSYTVSMG
                     LSLLLLILSGLWLWYGPKRLRKSRKQKTDI"
     gene            3980..4561
                     /locus_tag="PLO70_15090"
     CDS             3980..4561
                     /locus_tag="PLO70_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ75844.1"
                     /translation="MTEEAILQGCLRNDATAQRELYNRYSPKMLAVCYRFAHNREDAE
                     DMLQEGFIKVFLQIHTFENRGAFEGWIRRIIVHTCINILKKNKKFNESVDIIHATGLQ
                     IREESVPAIIQAKQVVECIRLLPIGYRTVLNLYAVEGYSHREIANMLDIEESTSRSQY
                     TRAKAMLEDILIRKRILQKPKQETDWLAAVSQR"
     gene            4593..6029
                     /locus_tag="PLO70_15095"
     CDS             4593..6029
                     /locus_tag="PLO70_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75845.1"
                     /translation="MESNFNNRDFERFLKQNADQYRMFPSENVWKGIHGTLHTRRRWY
                     GIALALLLLTTGAVTWVMLTGPSGRKNQVTINKPVTAKAVQKEQPVVITPLKVKTTNS
                     EVLIASPDNHQKNLFLANQVVEKNTIDVAGLQRSNAVVTVPGTPVSQTQAIAFSPAPI
                     SRQTASFNKEEVVKTNNPKNDAIFKNSINVDISIPSNVSAEKTLTEKNKNESLEKDIY
                     PLTIESVINSFKNVRKRKKLSWQLYVTPTISYRELKENKQFINAARSNLTATSVVYSP
                     DLNSIVTHKPDLGFQLGFTAGYALSKKITLIGGLQFNVSKYDIRAYNYPSEVATIALS
                     TASGGTNTVSAITNYRSAGGSRTANWLRNYYFSVSAPVGVELKLTGNKKTYWGISGTV
                     QPTYVLSNTAYVISTDYKNYAEVPSLTRKWNINTGFETFAGFSIGNTNWRVGPQVRYQ
                     TMSSFKKKYPVEEHLFDFGLKMGIQLNK"
BASE COUNT         2089 a         1101 c         1217 g         1651 t
ORIGIN      
        1 attattagca gccctggtaa taatacaatt tatacatcca aaaaaaaata aagcagaggg
       61 accacagccc aattatattg gaaattcttt tactgtacct gttgatgtaa aaccaatatt
      121 agataaagcc tgtaatgact gtcacagcaa caatagtaac tacccctggt atgcgaactt
      181 tcaacctatt cactggtggc tggaaaaaca cataaaagat gggaaaaaag agttgaattt
      241 tgacgaatat acaaaccgca gtcttcgtta tcaatatcac aaaatggaag agacaattga
      301 aatggtaaaa gaaggagaaa tgccattaaa ttcctacacc tggacacata aagacgcaaa
      361 actcactaag gaagaaaaaa taaagatcac cggttgggca gagtctgtta tggatacgat
      421 gaaggcaaaa ttccctatag acagcctcat caggaagaaa tagttctatc tttagtatta
      481 ctgatctctt gtgagaacca aagctaaaca aataagcggc atttttttaa ccctgcttgg
      541 gcttgttcct ttgctgttta tcctcttaat cactctcaag aaacatgata tccgtcacag
      601 gatgaaggaa aaaattggtc aacagcagct tcaaacaata gttttgtccg aagcagaagt
      661 tatctggatg gataaacatg aaatatgggt aaataacagc atgtttgata ttcataccaa
      721 aaagctggat actggcattt acacttttac cggattatat gatgaagaag aaaccatgct
      781 ggtagaaaaa gaaagaactg cagccggaga aaataatgat caaaccaaac tacttgcaca
      841 acttttcaaa tgcttaccgg ttttttgtaa ccaggataca gatattgttg acctcaattt
      901 tcaatataat tcttacaatc agatagtaca ccccaatcca atataccagt tcagggaaat
      961 actcaccccg ccaccgaagg tttgatattc aaatattcag accaaaccaa gtcataaaaa
     1021 aaatacaatt tatgagtaat aagtatgtgc ttattctgtt atgctcattt tgcatggctg
     1081 tgaatggcta tacacaggaa gatacaacaa ctgaaaaaac actgggagaa gtcatcgtgt
     1141 ctgtaaacaa atgggaacaa aaacttaatg aattgcccaa caaaattgta aaagtcaata
     1201 gatatgacat cttaaaaaat aatccccaga cggcggctga tttattagga cagactggta
     1261 cagtgttcat tcaaaaaagt cagttgggtg gtggcagtcc gatgattcgt gggtttgcaa
     1321 ctaacagggt gctgcttgta attgatggtg ttcgaatgaa taatgctatt taccggagcg
     1381 gcaaccttca aaatgtaata agtattgatg cattgagtac tgaaacagcc gaagtaatat
     1441 ttggccctgg ctctctcatt tatggcagcg acgccattgg tggtgtgatg gattttcata
     1501 ccctggatgc ccggttttca aaagataaaa agatagtggt gaaaggtagt gtactgggca
     1561 ggtattcaac agctaataaa gaaaatactc ttcatgcaga tattaatctg ggttggaaaa
     1621 aatggtcatt gttgtcatct ctttcttaca gcaagtttga cgatttaaaa atgggaaaga
     1681 atggtggaca ggacagctat ctgcgaccag agtacgtcga aaggattaat aacaaagaca
     1741 gtattgtaaa aaacggcgac ccccgcattc aacggttcag cggttatgat caattgaatt
     1801 ttttacaaaa aatacggttc aggccaaatg agcattggga tttactatac agttttactc
     1861 atgcaaaaac aggcgttgcc ccaaggtatg atcgtttgat tcaatacagg aacggagcgc
     1921 tgcgttttgc agaatggtat tatggtccta tgctatggaa tatgcacacc ctgcagattt
     1981 tacattctaa aaaaacaagc atctatgaag atgcccggtt aacagtggcc taccaggatt
     2041 ataaagaaag tcgtattgac cgctcaaaag caaatatcaa tcgtaacagg caggttgaat
     2101 cggttgatgc catcagtgcc aactgggatg caactaaaaa tacaggaaaa ggacagttat
     2161 tttatggact tgaatatgtg cacaacaaag tcggttcgtt tggagaaaga accaatatca
     2221 gtactaatgt tgtaacacca atggtaagcc gttatcctga tggcagcacc tggagtacta
     2281 ctggcatcta tggcagttat aaaataaatc tgcatacaaa ggtaactttg acgacaggtt
     2341 tgcgatacag ttataataca ttagatgcca ctttcgatac tacatttatc aagtttccgt
     2401 ataagaatgc aaaactgaga gacggggctc tgactggtaa tgctggactt gtattccgtc
     2461 cggcagaggg ctggcaattg aatggaaata tatccactgg ctacagaatg cccaatgtag
     2521 atgatattgg aaaactcttt gaaagtgtac cggggaacat tagcgttcca aatcctgatc
     2581 taacacctga atatgcatgg aattttgaaa tgggcattat taaaactatt cctaataaat
     2641 ttcgcattga gttaaatggg ttttatacta ttttaaaaaa tgctattgtt cgacgtccaa
     2701 ccacatttaa tggctcagat agtattttgt ttaacgggat tttaagtcag gtagaagcat
     2761 tgcaaaatgt ggcaaaagca accgtttcag gttttcaggc aagtgcagaa ttttatttta
     2821 caaaatcggt atcgttgcaa actcatgcga actggattag tggaaaagaa acagacgata
     2881 taaatgatga gcaggtgccg cttcggcatg ctccaccgtt ttatggcagt acatcaatac
     2941 gctacagatt aaaaaaaata tttgtggaag catctgcaca gtataatagt gaaatagaaa
     3001 atgtaaagct ggcaccttct gagcaggtta aaacagatat ttacgcaaaa gatgcagatg
     3061 gtaaacctta ttcgccagga tggcatacag taaatctgaa agccacttac cagttatcaa
     3121 agaatctact ggttacaact ggttgggaaa acataaccaa ccaacgttat cgcccttatt
     3181 cgtccggtat agtggctgct ggttctaatt ttattatttc cctgagagtt tcactttaac
     3241 caaagaaggg tttgtataaa aagcaagccc ctaatttttt taattatgag cagtaccaaa
     3301 caacaagcta aatggattcg caggttccgc tggttacacc ggaagattgc tattttttta
     3361 tttatattct tttcccttat tgctataaca ggtcttttac tgggaatcaa aaaacaaacc
     3421 ggattattag ctccgactca aaaaggaatt tccgcagatc tttccacctg gctaccaatc
     3481 gatagtctgc agaaaattgc aatcaaatac ctgcaggatt ctgtatctgt aagtctaaac
     3541 tcagaaattg acaggattga tatcagacca gaaaaaggga ttgcaaagtt catttttaag
     3601 gaccacttta acggcttaca gctggatggc agcaccggaa gactactttc aatagaaaaa
     3661 aggagaagtg attttattga aaatctgcat gacggatcaa tactggataa gttattgggg
     3721 accggtggcg atcaggtaaa agtcagctac actgtcagta tggggcttag tcttttactc
     3781 cttattttat ctggtttatg gctttggtat ggacccaaaa ggttgagaaa aagccgtaaa
     3841 caaaaaactg acatataaat aaatacgtag aatcacccta taaatgatta aaattctatt
     3901 aacctgtcta aaattttaca ttttggtgca gcaatcgtac agaaaaaatg tctaatttac
     3961 aggcaaccaa agggtagtta tgaccgagga agccatttta caaggttgtt tgagaaatga
     4021 tgccacagcc caaagggagt tgtacaatcg gtacagtcct aaaatgctgg ctgtttgtta
     4081 tcgttttgct cacaatcgtg aagatgccga ggatatgttg caggaagggt ttatcaaggt
     4141 ttttctacag attcacacct tcgagaaccg aggagcattt gaaggatgga tacgacggat
     4201 tattgttcat acctgcatca atattctcaa aaagaataaa aagttcaatg aaagtgttga
     4261 tataattcat gccacagggt tacagataag agaggaaagt gtgcctgcta tcatacaggc
     4321 caaacaagtg gtggaatgta taagactttt gccgattggc tacagaactg tattaaatct
     4381 ttatgctgta gaaggttaca gtcacaggga aattgccaat atgctggaca tagaagagag
     4441 taccagcagg agccagtata caagggcaaa agcaatgctg gaagatattt taattcgtaa
     4501 gaggatatta caaaaaccaa aacaagaaac agattggctt gctgccgtta gccagagata
     4561 aatataacag tacccttaat tgctgactgg ttatggagag taattttaat aatagagatt
     4621 ttgagcggtt cctgaagcaa aatgctgacc agtaccggat gtttccatct gaaaatgtat
     4681 ggaaaggcat tcacggaact ttgcatacac gcaggaggtg gtatggcatt gccctggcat
     4741 tattattact tacaacagga gcagtcacct gggtcatgtt aaccggccct tcaggcagaa
     4801 aaaatcaggt aactattaat aagcctgtaa ctgctaaagc tgtgcaaaaa gaacaacccg
     4861 ttgtcattac ccctttaaaa gttaaaacta caaatagcga agtattaata gcatctcctg
     4921 ataatcacca aaaaaatctc tttctggcaa accaggtagt agaaaaaaac acaattgatg
     4981 tggcaggatt acagagatcg aatgcagtgg taacagttcc cgggacacct gttagtcaaa
     5041 cccaagctat tgctttttcc cctgccccaa tatccagaca aactgcttca ttcaataaag
     5101 aagaagttgt aaaaacaaat aatccaaaga atgatgcgat atttaaaaac agtatcaatg
     5161 tagacataag tatcccatct aatgtttcag cagaaaaaac attaacagag aaaaataaga
     5221 atgaaagtct tgaaaaagac atatatcctt taactattga aagtgtaata aactccttca
     5281 aaaatgtcag aaaacgtaaa aagctgtcat ggcagttata cgttacccca acgatcagct
     5341 atcgtgagtt aaaagaaaac aaacaattta taaatgctgc ccgttcaaac ctcacagcta
     5401 cttcagttgt ctattctcct gatctgaata gcattgtaac ccataaaccc gatttaggat
     5461 ttcagttagg ctttacagca ggttatgctc tctccaaaaa aattaccctc ataggaggat
     5521 tgcagtttaa tgtcagtaaa tatgacatta gagcttacaa ttatcccagt gaagtggcta
     5581 ctattgcact cagcacggcc agtggcggca caaatactgt ctccgctata acaaactaca
     5641 gaagtgcggg aggaagcaga acagcaaact ggctcaggaa ttattatttc tccgtttctg
     5701 cacctgttgg tgtcgaactt aagctgactg gcaataaaaa aacctattgg ggtatcagcg
     5761 gcactgttca accaacttat gttttgagca atactgcata tgttatttca acagactata
     5821 aaaactatgc agaagtacca tcgctgaccc gtaaatggaa tattaatacc ggttttgaaa
     5881 cttttgcagg tttctcaatc ggcaatacga attggagagt tggtccgcag gtacgttatc
     5941 agacaatgtc gagtttcaag aaaaaatatc ccgtagagga gcatcttttt gattttggtt
     6001 taaaaatggg catacagctc aacaagtaga acttacacta actgctgctt tgcaaaaa
//
LOCUS       DAORTI010000173         5939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_15935_length_5939_cov_6.222298, whole genome shotgun sequence.
ACCESSION   DAORTI010000173 DAORTI010000000
VERSION     DAORTI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15935_length_5939_cov_6.222298"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLO70_15100"
     CDS             complement(<1..647)
                     /locus_tag="PLO70_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQZ75846.1"
                     /translation="MRYLKILPGLLLLGLLCSPVLSFSQVSPKLPPVTTVSPVAPVDT
                     LTDVEILGSRHLTILKLNDSTTLQILAGNVRLRQGKSLFNCDSCVLNNDNKTFEAWGK
                     VHINDADTINVYANHLRYLIDRKIAYLDGAVKLTDGKGTLTTPDLEYDMSTDIGIYKN
                     GGKVVNKTTTLTSKEGYYYAGLRDIYFKNNVELKDPSYFIKTDSLLYNTQSQTTQ"
     gene            complement(647..1639)
                     /locus_tag="PLO70_15105"
     CDS             complement(647..1639)
                     /locus_tag="PLO70_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQZ75847.1"
                     /translation="MKISNETKVGILSIVALTILIVGFNFLKGKDVFNRSDKIYAVFK
                     DLGTLEKSNEVKINGLPIGIVYDKQEKDKNVSAIVVTINLTRNVNIPKNSVAFISSSL
                     VGSSFIVIERGDSPVMMEIGDTLETRTETGILGDVKAQLNPTLAKVRDVLDSLQTTLS
                     GVNGMLSSENKGYLKQTLTNLSNASNALNGLLDNQTSALAKTLNNAQAISESLKNNTG
                     DITATIGNAKKATEKLSALELQPTIDSLNAMIAQLKTTAAKLSSKDGTLGALINDKTL
                     YNKLNDAILSAEILMDDLRTHPKRYVNLSIFGKKDKSGPLTSPAKKDTVPAGDN"
     gene            complement(1775..2815)
                     /locus_tag="PLO70_15110"
     CDS             complement(1775..2815)
                     /locus_tag="PLO70_15110"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQZ75848.1"
                     /translation="MIKRTFLVFLAAAFTISFFSFKGDDISTTPRKQKVVKTIVIDPG
                     HGGSDAGAKGQYSYEKDICLDVSLKLGKMIEKEFPDIKLLFTRTTDTYPALHARADYA
                     NNNKGDLFLCIHVNSAPAQRKSELTGYKTVTYYTGKGKNRKKKTKQVPQYRYYNLPSK
                     AKGTETYIWGAHKSEEKELAVRENAPMLAEENFEQNYGGIDPNSPEFIALSLLKTKQY
                     FKRSATLAGYVQDEFVKVGRVDRDVKQRGVGIWVLQATAMPSVLIETGFISNPEEEKY
                     LNSQEGQNELSECIIRALRKYISWLEEKQEDDKKEVKDDKITESDRKAAAANTKAFLE
                     MVEANEKKRQAK"
     gene            3031..5703
                     /locus_tag="PLO70_15115"
     CDS             3031..5703
                     /locus_tag="PLO70_15115"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQZ75849.1"
                     /translation="MPGHFFYTPLTYFADTVKPLIKKDAINANNVAGTDTAKLPAADT
                     TKLPGIDSIIKQKIDTFSLKVSKDSLEAPLKYEAADSVVVLVQAKKIILYGKTKTEYK
                     DIVLTAPKVEVDQVTQMVTAVNSKDSTGEVIETAKFKSEESEFTSDTIQYNFKSQVGL
                     TKNTYTQQGEILVIGEVAKKVNENTTFIKRARFTTCLLDEPHFAFVTPKMKVINQKLA
                     ISGPAHPEFEGVPVPVYLPFGFYPLSQGRHSGLLRPQFMTDEVRGLGLQGLGYYKVLN
                     DFWDAQITGDIFSYGEWSLGLRTTYRKIYKYNGGFNLSMRSSKVNFKGDPDFSRSQVY
                     NITWNHSLDTRARPGINFTANVNAGSTKFNQLIPGNPFINYQNLMGSSITFSKTWKNK
                     PFNLSLSATHSQNNNLRLINLSLPNAAFTVGNVYPFQSKKGTGSIKWYEKLAVAYAGN
                     FSNNISFYDSLTYGKNGVKPFLKYLLDTAQWTAQHSIPITLSLPPILGGRIIVAPGIN
                     YGQTWIQRTTNYSWNPTLKKVDTITSKGLFIDQRGSVSLGFNTAVFGTVQFKKSRIMA
                     LRHVIRPNMGFSYTPDLNKGRIKSTQVDTTGRVLFYNEIGGGFLYNASTRRSAAMNFG
                     VDNNVEMKWRSKKDTGELAIKKIRVIEGFGFNGSYDFIRDSMNLSPISIYFRTTLLEK
                     ININASTTLNPYQTDARGYDIAKYAWQGGKFKLGRLTTGNISVSTSFQSKPKDAKKEE
                     ERKKQSEQRLNDPALLADQQRLLDYMQQNSSEFVDFNIQWNLSLSYSLSFVNIIKPDF
                     SGFEKDFTSGISFNGGFNLTDKWKLSGSGSYDIDTKEVQYISLSINRDMHCWQLSINV
                     IPVGFTRSFNFSISPKAALLQDLKINRTRYFTSY"
     gene            complement(5697..>5939)
                     /locus_tag="PLO70_15120"
     CDS             complement(5697..>5939)
                     /locus_tag="PLO70_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQZ75850.1"
                     /translation="LKKFHGGAFKLAVETGTAIMPAVIFNTKKVLPANKPFFFWPSKV
                     AIHFLPPVSTANLTADQLKEKIFQITMEYYTSHQDQ"
BASE COUNT         1692 a         1177 c         1256 g         1814 t
ORIGIN      
        1 aactgggtgg tctggctttg tgtattatag agtaatgaat cggttttaat aaaataagac
       61 gggtctttta attccacatt gtttttaaaa taaatatctc ttaatccggc ataataatat
      121 ccttctttac tggtcagtgt ggttgtttta ttaaccacct tgccgccgtt cttgtaaata
      181 ccaatatcgg tcgacatatc atattcaaga tcgggggtag taagggttcc tttaccatcg
      241 gtgagtttaa cggcgccatc aaggtaagcg atctttctgt cgatcaggta gcggagatga
      301 ttggcatata cgttaatggt atcagcatcg ttaatatgca ctttccccca ggcttcaaat
      361 gttttgttat cattattcag tacacagcta tcacaattga aaaggctctt gccttgtctt
      421 aatcttacat tacccgccag aatctgcagg gtggtgctgt catttaattt taaaatggtc
      481 aggtgcctgg acccaagaat ttcaacatca gttagggtgt caaccggtgc tacgggagaa
      541 acagtggtaa ccggtggcaa tttcggagag acctgggaaa aagaaaggac agggctgcac
      601 aagagtccca ataataataa gccaggtaaa attttcaggt atcgcattag ttatcccctg
      661 caggaacagt gtcttttttt gcaggcgaag ttaacgggcc gcttttatct tttttaccaa
      721 aaatggaaag attaacgtag cgtttaggat gtgttcttaa atcatccatt aatatttcag
      781 cactcaggat cgcatcattg agtttgttat aaagtgtttt atcgttgatg agtgcaccaa
      841 gtgtgccgtc cttactggaa agtttggcag cggtagtttt caactgtgcg atcattgcat
      901 ttaatgaatc aatcgttggc tgcaattcaa gtgctgataa tttttctgtc gctttttttg
      961 cattaccaat ggtggcagtg atgtcgccgg tattgttctt aagactttca gaaatagcct
     1021 gagcattgtt cagggtttta gctaatgcac tggtttgatt atccagtaaa ccattcaggg
     1081 cattgctggc gttgctgagg ttggtcagcg tctgcttcag gtaccctttg ttttctgaac
     1141 tcagcatccc atttacacca ctcagggttg tctgtaagga atccagtaca tctcttactt
     1201 tggccagtgt ggggttcagc tgggctttta cgtctccgag gatccctgtt tcagtccggg
     1261 tttcaagggt gtcacctatt tccatcatta ccggtgaatc gccccgttca ataacaatga
     1321 aagaagaacc tacgaggctt gatgaaataa acgctactga atttttaggg atattaacat
     1381 tacgggtaag attaattgtt accacaatgg cactgacatt tttatctttt tcttgtttat
     1441 catagacaat accaattgga agtccgttga tctttacctc gttagatttt tcaagggtac
     1501 cgaggtcttt aaaaacagca tagatcttat cactgcggtt aaaaacatct ttccccttta
     1561 aaaaattaaa tccaacaata agtatcgtaa gggctacgat ggataatatg ccgactttag
     1621 tttcgttgct gattttcatt cgaatgcttt tgataaggtt aaaacctttc gggtgccaaa
     1681 ttagctaatt atttttaaac ggtacagcgg gggcggagta aaagaaaaaa ggatgaacca
     1741 gcgtccatcc ttttacttat caatcagtat aaaattattt ggcctgcctt ttcttctcat
     1801 tggcttccac catttcaaga aatgctttgg tattggctgc cgccgctttt ctgtcactct
     1861 ctgttatttt atcatccttc acctctttct tgtcatcttc ctgtttttct tccagccagc
     1921 tgatatactt tcttaatgcc ctgataatgc attctgaaag ttcgttttga ccttcctggc
     1981 tgttcaggta cttttcttcc tccggattgg agataaaacc ggtttctatc aaaacgctgg
     2041 gcatagcagt ggcctgcagt acccagatac ccaccccacg ttgttttaca tcacggtcca
     2101 cacggcctac ttttacaaac tcatcctgta cataaccagc cagggtagcg ctgcgtttga
     2161 agtattgttt tgttttcagc aacgataaag caataaattc tggtgagtta gggtcaatgc
     2221 caccatagtt ctgttcaaaa ttttcctcgg ccagcatcgg tgcattttcc cgaaccgcta
     2281 attctttttc ttcactttta tgagcacccc atatataggt ttctgttcct ttggccttac
     2341 ttggcaaatt gtaataacgg tattgaggaa cctgtttggt tttctttttc ctgttctttc
     2401 cttttccagt ataataggta actgttttgt aaccggtcaa ttcagatttt cgctgtgccg
     2461 gtgctgagtt cacatgtata cataagaaca ggtcaccttt gttattattg gcatagtcgg
     2521 ccctggcatg taaagccggg taggtatcag tggtacgtgt aaaaagcagc ttgatgtcgg
     2581 ggaattcttt ttcgatcatt ttacccagtt tcaacgatac atccaggcag atatcttttt
     2641 catacgaata ttgtcctttg gcaccggcat cactgccgcc atggccggga tcaatgacaa
     2701 tggtcttcac tactttttgt ttccggggag tagtagaaat atcatcgcct ttaaaggaaa
     2761 agaaactgat ggtgaatgcg gctgctagga aaacaagaaa ggttcgtttg atcatagttt
     2821 ttcattttac gtcctgtgga ctttaatcaa agggtggcag aatattcaca aattgtttag
     2881 gcttatcaat aggtctccaa tctataaaga tttacatttg cttcctacct gatgaagcaa
     2941 ctgtacaaat ttaggtcaat ttactttttt tacgggatac tgactgcggt tatattttgc
     3001 acactaacgt ggaaaacagc tgctaacggt gtgccaggcc atttttttta cactccttta
     3061 acatacttcg cagatacagt aaagccacta attaaaaaag atgcgatcaa tgcgaataac
     3121 gttgcaggta cagatactgc aaaattaccg gctgcagata caacaaaatt gccgggtatt
     3181 gattcaatta tcaaacaaaa gatcgatacg ttctcactaa aagtatccaa agattcgtta
     3241 gaagcgccgc taaaatatga agcagctgac tctgttgttg tactggtgca ggccaaaaaa
     3301 attatccttt atgggaaaac caaaacggag tacaaggata ttgtattgac ggcccccaaa
     3361 gtagaagtag accaggtaac gcaaatggtg acggcggtca atagtaaaga cagcaccggg
     3421 gaagtaatag aaacagcaaa attcaaaagt gaagaaagtg aatttaccag tgacacgatt
     3481 cagtataatt ttaagtcgca ggtgggactt actaaaaata cttataccca gcagggagaa
     3541 atattagtga taggtgaagt ggccaaaaaa gtaaatgaga ataccacgtt tattaaaagg
     3601 gcaagattta ccacttgttt actggatgaa ccgcactttg catttgtaac acccaagatg
     3661 aaagtgatca accagaaact ggctatatcc ggaccggctc acccggagtt tgaaggggtg
     3721 cctgttccgg tatacctgcc atttggtttt tacccactta gccagggaag acattcaggt
     3781 ttactccggc cgcagtttat gacggatgaa gtaagagggc tgggtttaca gggccttgga
     3841 tattataaag tgctgaatga tttctgggat gcacagatca ccggagatat tttttcatat
     3901 ggagaatggt cacttggttt gagaacaacc taccggaaaa tatataagta taacgggggc
     3961 tttaacctga gcatgcgatc ttccaaagtg aattttaaag gggatcctga tttttcaaga
     4021 agccaggttt ataatattac ctggaatcac tcgctggata caagggcaag accgggaata
     4081 aatttcactg ctaatgtaaa tgctggttca actaaattta accagctcat tccgggtaat
     4141 ccctttatta attaccagaa cctgatggga tcttctatca cattcagtaa aacatggaag
     4201 aacaaacctt ttaacctgtc cctcagtgcc acccactcgc agaataataa tctcaggctt
     4261 atcaatctga gcctgcccaa tgctgcattt acggtaggta atgtttatcc gtttcaaagt
     4321 aagaagggta ccggttctat aaaatggtat gagaaactgg ccgttgccta tgcaggaaat
     4381 ttcagtaaca atatcagctt ttatgattca cttacctacg gaaagaatgg ggtaaaacct
     4441 tttttaaaat acctgctgga tactgcacaa tggactgcac aacatagtat tcccattaca
     4501 ttatccctgc cgccgatatt gggcggaagg atcattgtgg ctcccggcat caattatggc
     4561 cagacctgga tacaacggac aaccaattat tcctggaacc ccaccctgaa aaaagtggat
     4621 actataacat caaaaggttt gttcattgat cagcggggaa gtgtttcttt aggatttaat
     4681 acagccgtgt ttggaacggt acagttcaaa aagtcaagaa tcatggcttt acggcatgtg
     4741 atacgaccca atatgggatt cagttatact cccgatctta ataaggggcg tataaaaagt
     4801 acacaggtcg ataccacggg aagggtttta ttttataatg aaattggcgg tggctttctg
     4861 tacaatgcat ctacccgcag gtcggctgct atgaactttg gtgtggataa taatgtagaa
     4921 atgaaatggc ggtctaaaaa agataccggt gaactggcga tcaaaaaaat aagagtcatt
     4981 gaaggttttg ggtttaacgg gtcctatgat tttatcaggg attccatgaa tctgtcaccc
     5041 atcagtattt atttcagaac cactttattg gagaagatca atatcaatgc cagcactact
     5101 ttgaatcctt atcaaacaga tgcgagggga tatgatatcg ccaaatatgc atggcaggga
     5161 ggaaagttca aactgggcag gttaactacc ggtaatattt ctgtatctac cagttttcaa
     5221 agtaaaccca aagatgccaa aaaggaagaa gaacggaaga aacaatccga gcaacgccta
     5281 aatgatcctg ctttgttagc tgatcagcag cggctgctgg attatatgca gcaaaattct
     5341 tctgaattcg tagacttcaa tattcaatgg aacctgagcc tgagttattc tttatctttt
     5401 gtaaatatca tcaagccaga cttcagtggt tttgaaaagg attttacttc gggaataagt
     5461 tttaatggcg gtttcaatct tacggataag tggaaattgt ccggaagcgg cagttatgat
     5521 atcgatacca aagaagtaca atacatttct ttatctatca acagggatat gcactgctgg
     5581 caattatcta tcaacgtcat cccggtaggc tttacccggt cctttaattt ttccatcagc
     5641 cccaaagcgg cgttgctgca ggacctgaag atcaaccgta cccggtattt caccagttat
     5701 tgatcctggt ggctggtata atactccatt gttatctgga atatcttttc ttttaactga
     5761 tcagcggtca ggtttgcggt acttaccggt ggaagaaaat gtatcgccac ttttgatggc
     5821 cagaaaaaga agggcttatt tgccggcaat acttttttag tattgaaaat gacagcgggc
     5881 ataatagcgg ttccggtttc aacggccagt ttaaaggcac cgccatgaaa tttttttaa
//
LOCUS       DAORTI010000174         5884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_16182_length_5884_cov_5.611083, whole genome shotgun sequence.
ACCESSION   DAORTI010000174 DAORTI010000000
VERSION     DAORTI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16182_length_5884_cov_5.611083"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLO70_15125"
     CDS             <1..235
                     /locus_tag="PLO70_15125"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQZ75851.1"
                     /translation="SSAYSTINLTYYQVLNSSTSDNDKPSFGTRISAAFKTGWEWITS
                     LFVGLVSVWPLCLVTFFALIFYRRMKPAKVKQS"
     gene            296..985
                     /locus_tag="PLO70_15130"
     CDS             296..985
                     /locus_tag="PLO70_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQZ75852.1"
                     /translation="MAKKKLVVLSGAGISAESGLRTFRDSDGLWEGYNIEDVATPRAW
                     RKDPGLVLEFYNYRRKNVLDAQPNAAHYGLAELEKDFDVTIITQNIDDLHERAGSSNV
                     LHLHGEIFKMRSERNSSLIAEIKSDIKLGDVAADGAQYRPHIVWFEEPVPKIEEAIPV
                     VHNADIFVVAGTSLVVYPAAGLVNYIKPEVPIYIIDKKIPYISSKQKLTIIEMPATEG
                     VKQLKELLKAF"
     gene            complement(972..2483)
                     /locus_tag="PLO70_15135"
     CDS             complement(972..2483)
                     /locus_tag="PLO70_15135"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ75853.1"
                     /translation="MKTILLFGAGKSATVLIDYLIDNAAAEKWAIVVVDADLQLAKSK
                     IAGSAYAKAVSFDITNTDERVQFIRSADIVISLLPPHLHIEVARDCVKLQKNLLTASY
                     VDESIKELQAEIEKNKLFFLYEMGLDPGIDHMSAMQLIDHIHANGGTITSFKSHCGGL
                     VAPESDDNPWHYKISWNPRNIIMAGKAGALYRENGEEKKQVYEELFTADRLVDIPELG
                     FLSWYPNRDSLSYTSLYGLENTATFIRTTLRHPDFMYGWKNIIELKLTDETPQYETDG
                     KTLQQVFKEHMDKNGFGDWINQKLTERFAETKGMLENLMQLMEAENEADKVGETMPES
                     FMTADENGNLQEVEIDTVKNNAAAFLASKMHEANLTLKQLFFLGLDDEETKVNKGMCS
                     PADILQFAVEKKLGLRPYDKDMIVMLHEIVYSTGSQQSAVSSSLVVKGENSLRTAMAK
                     TVGLPLGIATKLILSGKIKMTGLHIPTSKQIYQPVLKELELLGVKFNETVKKL"
     gene            2726..5323
                     /gene="gyrA"
                     /locus_tag="PLO70_15140"
     CDS             2726..5323
                     /gene="gyrA"
                     /locus_tag="PLO70_15140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQZ75854.1"
                     /translation="MEENLEPLETSDHNRIIPVNIEEQMKTAYIDYSMSVIVGRALPD
                     VRDGLKPVHRRILFAMNQLGLHYNKPYKKSARLVGEVLGKYHPHGDTSVYDAMVRMAQ
                     EWSMRYTMVDGQGNFGNQDGDGPAAMRYTEARLERLAEHMLDDIEKDTVDFQLNFDDS
                     EKEPTVLPTRVPQLLINGSSGIAVGMATNMMPHNLSEVIDGCIAFIDNREITVDELMQ
                     HVKAPDFPTGGIIYGMEGVKAAMHFGRGRVVVRGKLTVETKPSGREQIIITEVPYQVN
                     RDALTNRIGELVNEKVIEGIAHVNNESNNKEGTRVVIDLKRDAIANVLINQLYKLTDL
                     QTSYGINNVAIVKGRPRTLNLRELISEFVEFRHEVVVRRTQFELREAEKRAHILKGYL
                     IALDHLDEVIALIRSSATPDIAKDKLVNAGWGLDEIQAKAILELRLQRLTGMERDKIK
                     EEFDELMKLIAHLNELLSNEGMRFEVIKTELLEIKEKFGDTRKTEITYLDDEVKIKDL
                     IKEEDVVITISHLGYIKRTPADEFRAQRRGGRGVMGGKTRDEDYIEHLFVASTHHTML
                     FFTEKGRCFWLNIWEIPEGEKTGKGRAIQNLIQLPPDDKVKAIIDVRDLKDEEFVKGH
                     NIVLCTRKGIIKKTALEDFSRPRQNGINAITIVEGDELLDAKLTDGNSEIMMAVRSGR
                     AIRFSEEKVRATGRGAIGVAGIEADAAGDEVVGMICVNETDKASRTVLVVSELGYGKR
                     TPIDEYRFTNRGGKGVKTINVTDKTGSLVGILAVATIEDLMITCKSGVTIRMPVSGIS
                     EQGRATQGVKLIRLDEGDEIAAITQLTDQENNGKEENEEEGNQQPDSGGDNATSADAT
                     SSTESETES"
     gene            5372..>5884
                     /locus_tag="PLO70_15145"
     CDS             5372..>5884
                     /locus_tag="PLO70_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75855.1"
                     /translation="MKKIILILTTFLVATGMYAQKYDAIRNTALLQQFQKAKDDLDKA
                     MTNEKFTSKAEAFILKAYVYASLAMDQSKKDTPEASTLLHEADAAFNKYKTMDASLAL
                     VTDQVYQNGAVNLYSGFYTLGYNDYKDKKWNEGYTLLKKAMEYSDLLIDKKLISSPID
                     TNLLILAGITA"
BASE COUNT         1873 a         1099 c         1273 g         1639 t
ORIGIN      
        1 ttcttccgct tacagtacaa taaaccttac ttattaccag gtattaaatt cttctaccag
       61 tgacaatgat aagccgtcat ttggcacaag gatcagtgct gcattcaaaa caggttggga
      121 atggatcact tcactatttg ttggcctggt gtctgtctgg ccactctgtc tggtcacatt
      181 ctttgctctt attttttaca ggagaatgaa accagctaaa gtgaagcaat cctgagaaaa
      241 catgattgtg aagtcacttg tttgtacagg tggctttgct gttttacatt tgggcatggc
      301 aaagaagaaa ttggttgtac tgtccggagc aggcatcagc gccgaaagtg gtttaaggac
      361 ttttcgtgac agtgatggac tttgggaggg atacaatata gaagatgtgg ctactccaag
      421 ggcctggcga aaagaccccg ggctggtatt ggaattctat aattaccgca ggaaaaatgt
      481 attggatgcc caacccaatg ccgcccatta tggcttagcg gaactggaaa aggattttga
      541 tgttaccatc atcactcaaa acatagatga cctgcatgaa agagccggtt ctagcaatgt
      601 tcttcatctg catggcgaaa tctttaaaat gcggagcgaa agaaacagtt cattgatcgc
      661 tgaaatcaaa agcgatataa aactggggga tgtggcagcg gatggagcgc aataccgtcc
      721 gcatattgta tggtttgaag aacctgtccc gaaaattgaa gaagcgattc cggtagtaca
      781 caatgcagat atttttgtag tggcaggtac ctcattagtg gtgtatcctg ctgcaggcct
      841 ggtaaactat attaagccgg aagtcccgat ttatataatt gacaaaaaga ttccttatat
      901 ctcatcaaaa caaaaattga ctatcattga aatgccggcc accgaagggg taaaacaatt
      961 gaaagaatta ctcaaagctt tttaacagtc tcattaaatt ttacacccag caattctaac
     1021 tctttcaata caggttgata gatttgtttg gaagtaggta tatgcaaccc ggtcatttta
     1081 atttttccgc tcaaaatcag tttcgttgct atgcctaaag gaaggcctac tgttttagcc
     1141 attgcagttc ttaaactatt ttctccttta acaactaaag aactgctgac tgctgactgc
     1201 tgacttcctg tgctgtatac aatttcatgc agcataacaa tcatatcctt atcataagga
     1261 cgtagtccca gttttttttc tactgcaaac tgcagaatat cagccgggct gcacattcct
     1321 ttattcactt ttgtttcttc atcatccaat cccaaaaaga aaagctgttt caatgtgaga
     1381 tttgcttcat gcattttaga agcgaggaaa gcagcggcat tattctttac tgtgtcaatt
     1441 tccacttcct gcagatttcc attttcatca gccgtcataa atgattcagg cattgtttca
     1501 ccaaccttat cagcttcatt ttccgcttcc attaattgca taaggttttc aagcatcccc
     1561 tttgtttcgg caaaccgttc cgttagtttt tggtttatcc agtcaccaaa accgttttta
     1621 tccatatgtt ctttgaacac ttgttgcagg gttttgccat ctgtttcata ttgcggtgtt
     1681 tcatcggtga gtttaagttc gattatattt ttccaaccat acatgaaatc agggtgtcgc
     1741 aatgtcgtgc ggataaaggt ggctgtattc tccagtccat acagggaagt ataactaagt
     1801 gaatcccggt tgggatacca gctcaaaaaa ccaagttccg ggatatcaac taatctgtct
     1861 gctgtaaaca attcttcata gacctgtttt ttttcttctc cattttcccg gtaaagagca
     1921 cctgctttcc cggccataat gatattacgg ggattccagc ttattttata atgccaggga
     1981 ttatcatcac tttcaggggc tacaaggcca ccacaatgcg atttgaatga agtgatcgtt
     2041 ccgccatttg catgaatatg atcaatcagt tgcatcgcac tcatatggtc aataccggga
     2101 tcgaggccca tctcatacag gaaaaagagt ttattctttt caatctcagc ctgtaattct
     2161 tttatgcttt catcaacata agaagccgtc aataaattct tttggagctt tacacaatcc
     2221 ctggccactt caatatgcag gtgaggtggt aataatgaaa ttacaatatc ggctgaacga
     2281 ataaactgta ctctttcatc cgtattagta atatcaaacg atactgcttt tgcataggca
     2341 gaaccagcaa tttttgattt tgccagttga aggtctgcat ctactactac gatagcccac
     2401 ttctctgcag cggcattatc tatcaaataa tcaattaata cagtagctga ttttccagcc
     2461 ccaaacaata gtatggtctt cattggtaat gctatttcaa aaaccgaaaa ttaggcctaa
     2521 aaaatcataa ttagtgtgga aatgctgctt gtttttcaat cggttaacta atccacacga
     2581 gtggataaaa tagctcaaaa aacacctcct attatcaggt tttcagggca taaaaaagta
     2641 gatttgcaaa ccctgaaaaa gccccttttt tacgggtttg accgactcaa caatcaatta
     2701 aaaaaacaga aggaaattca ctcaaatgga agagaactta gagccactgg aaacgagcga
     2761 ccataaccgg ataattcctg ttaatataga agagcagatg aagacagcct acattgatta
     2821 ctccatgtcg gtaattgttg gtcgtgccct tcccgatgta agagatggat tgaaacctgt
     2881 tcatcgccgc atactgtttg cgatgaatca attggggctg cattataaca agccgtataa
     2941 aaaatccgcc cgtcttgtcg gggaagtgtt gggtaaatac catccgcatg gtgatacatc
     3001 cgtgtatgat gctatggttc ggatggcaca ggaatggagt atgcgttaca ccatggttga
     3061 cggacaggga aactttggta accaggatgg tgatggcccc gcagcgatgc gttatacaga
     3121 agccaggctg gaaaggctgg ctgagcatat gctcgatgac attgaaaagg ataccgttga
     3181 ttttcaactg aactttgatg actctgaaaa agagcctacc gttcttccaa caagagttcc
     3241 gcaattactg ataaatggct ccagtggtat tgccgttggc atggccacca atatgatgcc
     3301 gcataatctt tcagaagtca ttgatggctg tattgctttt attgacaacc gggagataac
     3361 cgttgatgaa ttgatgcagc atgtaaaagc tcctgatttc ccaacaggtg gtattattta
     3421 tggtatggaa ggggtgaaag cagctatgca ttttggaagg ggaagggtgg tggttagagg
     3481 taaattaacg gttgaaacaa aacccagcgg ccgtgaacag atcattatca ctgaagtccc
     3541 ataccaggta aaccgggatg ccctaactaa ccgcattggt gaattggtaa atgagaaagt
     3601 gatagaagga attgcccatg taaataacga atcaaataat aaagaaggca cccgggtggt
     3661 aattgatctg aaaagagatg caattgccaa tgtactgata aatcagttat acaaacttac
     3721 agacctgcaa acttcttacg gtatcaataa tgttgcgatc gtaaagggca gaccaagaac
     3781 actgaacctg agagagctta tcagtgagtt tgtggagttc aggcatgaag tggtggtcag
     3841 aagaacacaa tttgaattaa gagaggcaga aaaaagggcc catattttaa aaggttacct
     3901 catcgcactc gatcacctgg atgaagtgat tgcgctgatc cgcagttcag ccacaccaga
     3961 tattgcaaag gataaactgg taaatgcagg atggggatta gatgaaatac aggctaaagc
     4021 catacttgaa ctccgcctgc aacggttgac cggtatggag agggataaga tcaaagaaga
     4081 atttgatgaa ctgatgaaat taatcgcaca ccttaatgaa ttgttgtcga atgaaggaat
     4141 gaggtttgag gtgatcaaaa ctgaattgct ggagattaaa gaaaaattcg gggatacaag
     4201 aaaaacagag atcacttacc tggatgatga agtaaaaatt aaggacctca tcaaagaaga
     4261 agatgttgtt atcactatat cgcatttggg gtatatcaaa cgtaccccgg ccgatgaatt
     4321 ccgtgcacaa agacggggtg gcaggggagt gatgggtggc aaaacaagag atgaagatta
     4381 tattgaacat ctttttgtag catctaccca tcacactatg ttattcttta ctgaaaaagg
     4441 cagatgtttc tggttgaata tctgggaaat tccagaagga gagaaaacgg gaaaaggaag
     4501 agccattcaa aacctgatac aactaccacc tgacgataaa gtaaaagcta tcattgatgt
     4561 acgtgacctc aaggatgagg aatttgtaaa ggggcataat attgttttat gtacacgcaa
     4621 aggaatcatt aaaaaaaccg ctttagaaga ttttagccgt ccgcgacaaa atggtatcaa
     4681 tgcgattacc atagtagaag gtgatgaact gttggatgct aaacttactg atggtaattc
     4741 tgaaatcatg atggcagtaa gaagcggacg agctattcgt ttctctgaag aaaaagtaag
     4801 agcaacagga cgtggtgcca ttggcgtagc cggtatagaa gctgatgcgg caggagatga
     4861 agtggtaggc atgatttgtg taaatgaaac tgacaaagca tccagaaccg tattagtagt
     4921 aagcgagttg ggttatggaa aacgaacacc aattgatgaa taccgtttta ctaatcgtgg
     4981 cggtaaggga gtaaagacta ttaatgtaac ggataaaaca ggtagcttag tgggtatctt
     5041 ggctgtagca actatagaag atttgatgat aacttgcaag agcggcgtta caattcgaat
     5101 gccagtgtca ggtatcagcg agcagggaag ggccacacaa ggtgtaaaac taataaggct
     5161 ggatgaagga gatgaaatag ctgccattac tcaattaact gatcaggaaa ataatggtaa
     5221 agaggaaaat gaagaagaag gtaatcaaca acccgattct ggaggagata acgcaacctc
     5281 tgcagatgca acttcatcta cagaaagcga aacggaatca taatatttta aacaactagt
     5341 atcttattgg tcactaaaac aataaaatct gatgaaaaaa atcattctca ttcttactac
     5401 attcttagtg gcaacaggta tgtatgccca aaagtatgat gccatccgaa acactgcgct
     5461 tttgcaacaa tttcaaaaag caaaagatga tctggataaa gcaatgacaa atgaaaagtt
     5521 tacttcaaaa gctgaagctt tcattttaaa ggcttatgta tatgccagcc ttgcaatgga
     5581 tcaatctaaa aaagacacac cggaagcaag tactttatta catgaagccg atgctgcttt
     5641 taataagtat aaaacaatgg atgcttcatt agcattggtt accgatcagg tttatcaaaa
     5701 tggtgctgtt aacctttatt cgggtttcta cacacttgga tataatgatt ataaagataa
     5761 aaaatggaat gagggatata ctttgttgaa aaaggcaatg gaatattctg acttacttat
     5821 cgataaaaaa ttaatttctt cgcctattga caccaatctt ttgatattgg ctggcataac
     5881 agca
//
LOCUS       DAORTI010000175         5848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_16318_length_5848_cov_8.815294, whole genome shotgun sequence.
ACCESSION   DAORTI010000175 DAORTI010000000
VERSION     DAORTI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_5848_cov_8.815294"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1254)
                     /locus_tag="PLO70_15150"
     CDS             complement(85..1254)
                     /locus_tag="PLO70_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQZ75856.1"
                     /translation="MADKMSRQQFLQYTSSAGAAVLLSSLEGFALEKREKKLKVAIIG
                     CGSVSNRYIPHLQTSSLIEIVSLCDIKPERAIAQNKQYNVNAATYATIDEMLTGVPFD
                     MMVTLTDMQVHGALNKKALLAGKHVWSEKPMANTYAEGKALLDLAKSRKLRIWGAPAV
                     VNSPQFEFMSKMIQEGKLGRIASAHGQYGHTGPTWSAFFYEKGGGSMPDLGVYNMATL
                     TGLLGPARSVMAMTSIVKPERTVDDKGKIKVAAEDNAHVLLEHDKGVISHVMCGFNYF
                     DPHGHEAGNQSLHSIQIYGDYGNMRLIGYDWETNGVMLDTSWDKPAALMSKDKGGYEW
                     QEGATKAGEALLKGTEPRVNVEHALHVLEIIEAARKSSATGMKVKLKSKFPWPVV"
     gene            complement(1259..2698)
                     /locus_tag="PLO70_15155"
     CDS             complement(1259..2698)
                     /locus_tag="PLO70_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQZ75857.1"
                     /translation="MKRLPLLILILFIQLALINAQSVKKGKYGDGPDSIAPVGIVKGL
                     SDEQLLETVQRQTFRFFWHGAHPYSGLALERSNTVLAEHYWDYINEAWDEPNFSKTSF
                     GPDAGAIGGTGFGIMSTVVAVQRGWIGRDTAVRRLIKIADFLINADCYHGIYPHFMNG
                     RTGKTIKFDRLDDGADIVETSYLLMGFLCAREYFNKDNPREIYLRKRINQLWGAANWN
                     WHTNGEKKLYWHWSPNNGFDMNFPVWGYNECLITYIMAASAPNPAKAIPKEAYDGTWV
                     GSFGFKNGNEYYGYKLPLGNYDKDKGGPLFFEQYTFQGIDPNGLTDSLGNDYFLQGKN
                     HTLINRAYCIENPKKFKGYSDKVWGLTAGDSYKGYVAHSPENDRGVIQPTAAISSMPY
                     TPKESMEALKHFYYNLGDKIWSKYGFVDGFSIHHNWYAKSHLAIDQGPIIVMIENYRT
                     GLLWKLFMQIPDIQNGLKRLGFSSPYFKK"
     gene            complement(2709..4088)
                     /locus_tag="PLO70_15160"
     CDS             complement(2709..4088)
                     /locus_tag="PLO70_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQZ75858.1"
                     /translation="MKKSLAYHPKSICLFILCICLSGWVFSQKTVAVPAGSIPVSGIQ
                     PNLSDSALLDIVQRQTFRYFWDFAHPVSGMARERSNIGFGYGHETVTTGGTGFGIMSV
                     IVAVERNWISRDTAAKFMLKMVNFLLKANSYHGAFPHWMDGATGKTIPFSRKDDGADL
                     VETSYLFQGLLCARQYFNGDNKTERELRNRINWLWPDIEWNWFTNGGQEVLYWHWSPN
                     NGWAMNFPVRGFNECLIMYILAASSPHERYQVSPAVYHRGWAESNFFKNGKDYYGIKL
                     PLGFDYGGPLFFSHYSFLGLDPRGLKDRYADYWEQNRNHTLINREHCIRNPNKFKGYG
                     ENCWGLTASDTYNGYAAHSPTEDLGTISPTAALSAFPYTPEYSMKVLRHFYYDLGSKI
                     WSEYGFVDAFNETKNWYADSHLAIDQGPVIVMIENYRTGLLWKLFMSCPEIKTGIKKL
                     GFESPWLTK"
     gene            complement(4115..4996)
                     /locus_tag="PLO70_15165"
     CDS             complement(4115..4996)
                     /locus_tag="PLO70_15165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQZ75859.1"
                     /translation="MKPALTLKFGLLLLIVCLSFSVHAQQEITVMSYNIRLDVASDGE
                     NRWDARKEKVTGLMKFYEPDFIGTQEVMHHQLLFIKQELQHYQHIGVGRDDAKEKGEY
                     SAIFYNAAKFDLKQQATFWLSPTPDTVSKGWGANINRVCTYGLFQRKKTNKLFWVINT
                     HFDHQSELARIEAAKLIMKVIDSLNKKKNYPVIFMGDLNARPEQEPVMQVASVMDNAR
                     NISKQLPYGGLDTWNAFQFSKKPSGCIDYIFVSRNNGWQVKKFATLTDSYDMKYPSDH
                     FPVLAVLLLKKRQRRIQ"
     gene            complement(5013..>5848)
                     /locus_tag="PLO70_15170"
     CDS             complement(5013..>5848)
                     /locus_tag="PLO70_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQZ75860.1"
                     /translation="DAAETTLRNDINTLYNNVEWDWFRQGGQNVLYWHYSTDKGWIMN
                     HQIRGWNECLVTYVLAASSTTHGIPAIVYNNGWAGNGTNGFINGNSYYGHQLPLGPSY
                     GGPLFFAHYSFMGINPNGLTDAYANYATQVTNHSLINFKYCVANPKLWYGYSDSCWGL
                     TASDISGGYTASSPTNDVSVIAPTAAISSMPYTPVESMKALKFFYYVLGDKLWKEYGF
                     VDAFSLKDLWFANSFLAIDQGPMIVMIENYRTGLLWNLFTSCPEVKAGMLSLGFSAPY
                     L"
BASE COUNT         1648 a         1361 c         1067 g         1772 t
ORIGIN      
        1 accgtcttta ttatagtccg taatttgtgt tgctgataat tgaaatgaca tagtaagaga
       61 ttattgggtt acacatttat tcacttaaac taccggccac ggaaacttac tcttcagttt
      121 cactttcatt ccggttgcag atgatttcct tgctgcttca ataatttcca atacatgcag
      181 ggcatgttca acattgacac ggggctctgt tccctttagg agagcttccc cggcttttgt
      241 tgctccttcc tgccactcat aaccaccctt atctttactc atcagtgctg ctggtttatc
      301 ccacgatgta tccagcatca caccattagt ttcccaatca tagccaatca gccgcatatt
      361 tccatagtca ccatagatct gtatagaatg caaagattga ttacccgctt catggccatg
      421 cggatcaaaa taattaaacc cgcacatcac atggctgatc acgcctttat catgttccaa
      481 taaaacatgt gcattatctt ctgctgccac ttttattttc cctttatcat ccactgtacg
      541 ttcaggtttt actatacttg tcatggccat cactgatctg gcaggtccca gtaaaccagt
      601 cagtgttgcc atattatata cccccagatc aggcatacta cccccacctt tctcataaaa
      661 aaaagcagac caggttggtc ctgtgtgtcc atattgccca tgtgcactgg caatacggcc
      721 cagttttcct tcctgtatca ttttactcat gaattcaaat tgcgggctgt tgaccactgc
      781 cggtgcaccc catatacgta gcttcctgct ctttgcaagg tctaacaatg ctttcccctc
      841 tgcataagta ttggccatcg gcttttcact ccatacatgt ttaccggcca gtaatgcttt
      901 tttattaagt gcgccatgca cctgcatatc agtaagtgta accatcatat caaatggcac
      961 acctgtaagc atctcatcaa tagttgcata agtggctgcg ttgacattgt attgtttatt
     1021 ctgcgcaata gccctttcag gttttatatc acataggctt acaatctcaa taagggagga
     1081 tgtttgcagg tgtggaatgt agcggttgct tacactaccg caaccaataa tggccacctt
     1141 taattttttt tcccgctttt ccagtgcaaa tccttccagt gaagaaagca acacggctgc
     1201 gccagctgaa ctggtgtatt gcagaaattg ttgtcttgac attttatctg ccatactgct
     1261 attttttaaa atacggggat gaaaatccca atctttttaa cccgttttgt atatccggta
     1321 tctgcataaa cagtttccat agtaaacctg tacgatagtt ttctatcatt acgatgatag
     1381 gaccctggtc aatggccaaa tgtgatttag cataccagtt atgatggatg ctgaacccgt
     1441 ctacaaaacc atacttactc catatcttat ctccaaggtt gtaatagaaa tgctttaacg
     1501 cttccatact ttctttgggt gtgtaaggca tggaagaaat agctgcagtt ggttgtatta
     1561 cgccacggtc attctcggga ctatgtgcca catacccttt ataactatca ccggctgtca
     1621 ggccccatac cttatcacta taccctttga atttcttagg attctcgatg caataggccc
     1681 tgttgattaa tgtatgattt ttcccctgca ggaaataatc attacccagc gaatccgtaa
     1741 gaccgttcgg gtcaataccc tggaaagtat attgttcaaa aaacaacggg cctcctttgt
     1801 ctttatcata attacccagt ggtaatttgt acccataata ttcattacca tttttaaagc
     1861 caaaacttcc tacccaggta ccatcatatg cttctttagg gattgcttta gcaggattgg
     1921 gcgcagaagc tgccatgata taggttatca aacattcatt atagccccac acggggaagt
     1981 tcatatcaaa tccattatta ggactccagt gccagtacaa ttttttttct ccgtttgtat
     2041 gccagttcca gttagcagcg ccccatagct ggttgatccg tttgcgcaga taaatttctc
     2101 ttggattgtc tttattaaaa tattcccggg cgcataaaaa gcccatcagt aaataggatg
     2161 tctctactat atctgcccca tcatccaacc tgtcgaactt aatggttttc cccgttctgc
     2221 cattcataaa atggggatag ataccgtgat aacaatctgc attaattaag aaatctgcga
     2281 tcttaattaa acggcggact gcagtatctc ttcctatcca gcccctttga acagccacta
     2341 ctgtactcat gataccaaag ccagtaccgc cgatagcacc agcatcagga ccaaaagatg
     2401 ttttactgaa gttgggttca tcccaggctt cattgatata atcccaatag tgttctgcaa
     2461 gaacagtatt acttctttcc agcgccaaac cactataagg gtgtgcgcca tgccagaaga
     2521 atcggaaagt ttgccgttgt acagtttcca gtagctgctc atctgatagt cccttaacta
     2581 tacctaccgg tgcaatactg tctggtccat caccgtactt acctttcttc accgattgtg
     2641 cgttgatgag agcaagctgt ataaatagga tcaatattag caggggcaat cgtttcatgt
     2701 tatataattt attttgtaag ccaggggctt tcaaatccca gtttttttat ccctgttttg
     2761 atttcaggac aactcataaa taatttccat agaagtccgg ttctgtaatt ctcaatcatc
     2821 actatgacag gaccctggtc aatagcaaga tgactatccg cataccagtt ctttgtttcg
     2881 ttgaaagcat ccacaaaacc atactcactc catatcttac ttcccaaatc atagtaaaag
     2941 tgccggagta ctttcataga gtattcaggt gtatatggga aagcacttaa tgcagctgta
     3001 ggagaaatgg taccaaggtc ttcggttgga gaatgtgcag catagccatt ataagtatcg
     3061 ctggcggtca atccccagca attttcacca taacccttaa atttattggg attgcggata
     3121 caatgctccc tgtttatcaa tgtatgattc ctgttctgct cccagtaatc agcgtagcgg
     3181 tcttttaatc ctcttggatc aaggccaaga aaagaataat gagaaaagaa aagagggccg
     3241 ccataatcaa aacctaatgg caatttaatt ccgtaatagt ctttcccgtt tttaaagaaa
     3301 ttactttccg cccatccgcg atgatataca gcagggctca cctggtatct ttcatgaggt
     3361 gaagaggcgg ctaatatata catgatcagg cattcattaa aaccacgaac tggaaagttc
     3421 attgcccacc cattgttagg gctccagtgc cagtataata cttcctgtcc accgtttgta
     3481 aaccagttcc actctatatc tggccataac cagttgatgc ggttacgcag ttcccgttct
     3541 gttttgttat cgccgttaaa atattgccgt gcacacaaca atccctggaa taaataagaa
     3601 gtctcaacca gatcagcgcc atcgtctttg cgactaaaag gaattgtttt acctgtggcg
     3661 ccatccatcc aatgcgggaa agctccatga taggaattgg ctttcagtaa aaagtttacc
     3721 attttcaaca taaacttagc agcagtatca cggctgatcc aatttctctc tactgctaca
     3781 attacactca tgataccaaa accggtaccg cctgttgtaa ccgtttcatg cccgtaaccg
     3841 aaaccaatat tacttctttc tcttgccatg ccactaaccg gatgggcaaa atcccagaag
     3901 taccggaacg tttgtcgttg aacaatatct aacaatgcag aatccgaaag attgggttga
     3961 atacccgaaa ctggtatgga accggccgga actgcaacag ttttctgact gaatacccat
     4021 cccgataaac aaatacaaag aataaaaaga cagatacttt ttggatgata agccaggctt
     4081 tttttcatat gctgatttgt ttcgggttaa tattttactg aattctcctt tgcctctttt
     4141 ttaaaagcaa tacagctaaa accgggaagt gatcagacgg atatttcata tcataagaat
     4201 cggtaagtgt tgcaaatttt ttaacctgcc agccattatt ccttgaaaca aaaatataat
     4261 caatacaacc ggagggtttt tttgaaaact gaaaagcatt ccaggtatct aaacctccat
     4321 agggtaattg tttagaaata ttcctggcat tatccataac agatgccacc tgcattacgg
     4381 gttcttgttc cgggcgggca tttaaatcac ccataaaaat caccgggtaa tttttctttt
     4441 tgttcaggct gtcaatcact ttcatgatca gctttgcagc ttcaatcctt gctaactcac
     4501 tttgatgatc aaaatgagta tttatcaccc aaaatagttt gtttgttttt ttacgctgaa
     4561 ataatccata cgtacaaact ctgttaatat tggctcccca cccttttgaa actgtatcgg
     4621 gtgtgggaga taaccaaaag gtagcttgct gttttagatc aaatttggca gcattataaa
     4681 agatggcgct gtattcaccc ttttctttcg catcgtcacg gccaacgcca atatgctggt
     4741 aatgctgtag ctcctgttta ataaacagaa gctggtggtg catcacttcc tgtgttccta
     4801 tgaagtcagg ttcataaaac ttcatcaggc ctgttacctt ttccttacgg gcatcccagc
     4861 gattttcccc gtcagaagca acatccaacc ggatattata ggacattact gttatttcct
     4921 gctgggcatg taccgaaaac gataaacata ctattaacag caagaggccg aactttaaag
     4981 tgagagctgg cttcataaat tttaatagcg tattataaat atggcgctga gaacccaagg
     5041 ctcagcattc ctgctttgac ttccgggcaa ctggtaaata aattccacaa taaacctgtg
     5101 cggtaatttt caatcattac tatcattggc ccctggtcaa tagccaggaa cgaatttgca
     5161 aaccacagat ccttcaagga aaaagcatct acaaaaccat attctttcca tagtttgtca
     5221 cccagtacat aataaaaaaa cttcaacgct ttcatcgatt caacaggagt atatggcatt
     5281 gatgagatag cagcagttgg agcaattaca ctcacatcat tggtgggtga acttgcagtg
     5341 taacctccgg aaatatcact ggccgtaagt ccccagcagc tgtcactgta tccataccac
     5401 aattttggat tagcaacaca atatttgaaa ttaattaatg aatgattggt gacttgtgtg
     5461 gcatagttgg cataagcgtc tgttaatcca ttcggattaa tacccataaa agaatagtga
     5521 gcaaaaaaca ggggacctcc gtaggacggg cctaatggta actggtgacc ataataacta
     5581 ttgccattga taaacccatt cgtaccatta ccagcccaac cgttgttata aacaatggcc
     5641 ggtattccat gagtggtgga cgaggcagcc aatacatacg tcacaaggca ctcattccag
     5701 ccccggatct ggtgattcat gatccagcct ttgtctgtac tgtaatgcca gtagagaaca
     5761 ttttgtcctc cctgccggaa ccaatcccat tctacattat tgtataacgt attgatatca
     5821 tttcgtaaag ttgtttccgc tgcatcca
//
LOCUS       DAORTI010000176         5833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_16381_length_5833_cov_5.004154, whole genome shotgun sequence.
ACCESSION   DAORTI010000176 DAORTI010000000
VERSION     DAORTI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16381_length_5833_cov_5.004154"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            596..2062
                     /locus_tag="PLO70_15175"
     CDS             596..2062
                     /locus_tag="PLO70_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQZ75861.1"
                     /translation="MPTTSSLAKKIGFWSATALIIGSIIGSGVFMKPASMAGQLGSPI
                     WLTIVWVIAGLFSLFGALVYAELGAMIPETGGIYVYFRKMFGDFVAYLYGWAAFSVIN
                     TTGVAAIAFVCAQYADYFLHLPRFDAATEQAVIWHLPFIGDLHPLENFGVKSLAIAIV
                     AGLTWLNYRSVKAGSSFQLISTIVKIGVIAALVIGIFFFGNGSVENFFHSENPKQGSA
                     LLAGVIAALTGAFFAYDGWINITFIAGEIKQPQRNIPKSLFTGVIACIIVYVLVNQAY
                     LYVLPVEKIATSSLVASDAIAKAIGNAAGALVAAMIVICTLGAVNGNLMATTRITYAM
                     GKDKVFLPWAGKEHKKFQTPGNALWLHGGWIAVLIVSGSFDMLADMMVFISWIAYGLG
                     AVGIFMLRKKMPDAERPYKIWLHPFVTLLFITFTALFLVITVYNDVTNYIHHRQPVVN
                     SLLGILITALGIPLYFYYRNKNRKDDTALPDQQGSTFL"
     gene            complement(2030..4036)
                     /locus_tag="PLO70_15180"
     CDS             complement(2030..4036)
                     /locus_tag="PLO70_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ75862.1"
                     /translation="MAKHCLIIFLLLAVAPLLQAQQNCTHPGQTPVSAIPVCGNDPFT
                     MNTPTYCGNTLVPVSCPGGFTYTNINPNYFRMSCYSSGTLGFTIVPDANMNYDWQLFD
                     ITNRNPFDIFTDASLFVACNWSSEPGETGATDLGTDTIVCGGGGLNTYSKMPQIIQGH
                     TYLLMVCNQGGSGGSYQLTLTGGTASITDAIDPRMDYARASCDGTHIILRTNKKIVCN
                     TIAADGSDFRLSNGAAVIGAIPGSCTSGLGTDSIIITLAQPLANGTYALLLQNGTDGN
                     TIGDICNKFILFNESISFTVTDVQQTVVQEALAANCSPDAVEVVFSNPIRCSSIAADG
                     SQFIVTGPQAVTISGARGLRCANNTVSDIISLQLASPITTAGTYQVTLSTGSNGSPLL
                     DECGLPIAPGIVSFTVAGAASASFTYAANETCNATRFSFQHPGGNGVNSWNWTFGNAG
                     TSNQQNPVQNFPGGGQYTIQLIVSNGVCNDTATENITTGEVLQAAFEVQKIICPGDTL
                     KISNNSSGNIDQWQWQFGNGQFSTLALPSAIQFVATGNETLYTITLIAGNTQQNCRDT
                     ARQTVRVLSNCFIAVPSAFTPNGDGLNDYLFPLNALNADNLQFSVYNRMGQLVFYTKD
                     WTKKWDGRINGILQDTGVYAWLLSYTHNGTGEKVLQKGTTLLIR"
     gene            4135..4788
                     /locus_tag="PLO70_15185"
     CDS             4135..4788
                     /locus_tag="PLO70_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQZ75863.1"
                     /translation="MRTAIVTGASGNLGQAVVQKFLSEGYAVEGTGSLLMDAPHFHSV
                     TVDLVNEEAAAEWVQAVINKHKTIDVAVLTVGGFAMADIAGTSTAMIGQQYRLNFETA
                     YNVARPVFVQMIKQGTGTIFLIGSKAGMEAANSKGMIAYGLSKSLLFRLAELMNEEAA
                     GTAVNTMVIVPSTIDTPQNRAAMPAADFSKWQTPEAIADVIFSNTKHKDSNSPVIEL"
     gene            4849..5247
                     /locus_tag="PLO70_15190"
     CDS             4849..5247
                     /locus_tag="PLO70_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75864.1"
                     /translation="MKYMKWIGLAAAVLLIVSCFMPWVIIESRNITVSGVDATGTNYG
                     KPGYIHFVLLAFFIVFTLVPRLWAKRMNLLVVAFNAAWMLRNFLVIGTCKAGECPERE
                     TGFYLMIIASLIMLISALFPDIKMKNDTGN"
     gene            5384..>5833
                     /locus_tag="PLO70_15195"
     CDS             5384..>5833
                     /locus_tag="PLO70_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQZ75865.1"
                     /translation="MRKGFFILQLLISLAYYTNGQTVVISQVYGGGGNSGATYTHDFI
                     EIFNATGSPISLTGWSVQYNSAAGTGTWQVTNLSGTLAAGQYYLIQEAMGSGGSVPLP
                     TPDATGTITMAATNGKVALLNTTTALTGDMPVSASVIDLVGFGTANYF"
BASE COUNT         1562 a         1234 c         1379 g         1658 t
ORIGIN      
        1 tgaaagggtg aaggcattga aagaagagat gtataagtag gagtgagcag gaagcaatca
       61 atggagaaaa gaaaatagaa tgtttccttt cattacaatt gtcagcctga gcttgtcgaa
      121 ggcgggatta ccgaaggcgg gtttgtagaa gttgtcagcc tgagcttgtc gaaggcgggc
      181 ttaccgaagg caatcaaaaa ccagccatca taattttcta ctgctgctga taaaaaaaat
      241 gattgaagtg cttgcgaaaa cagtagcttg tttttacttt agcaatagat aataattact
      301 caaacgattt ccggaaatcc attcctaccg tacctgttaa tccttccggg aaaaataagt
      361 atccggccgg gaggtatgtc ttagcaataa gatattcggg ttgaagccac tgtaaaatga
      421 tttgaagagg cagtttcaaa aaataaacaa atttgtcaca ttgagtttat cgaaatgagt
      481 acaaatttgt ttccccatca ttgtcctgtt tcggctttgc tcaacatgac aatgatgggg
      541 cttttgagac agcctctttt tttattgata agtatgctta tcttcctttc ctgttatgcc
      601 aaccacctct tcgctcgcca aaaaaatagg attctggtct gccactgcac tcattattgg
      661 aagtattatt ggctccggcg tttttatgaa accggccagt atggccgggc aattgggttc
      721 tcccatctgg ttgacaatcg tctgggtcat tgcaggttta ttttcccttt ttggcgccct
      781 cgtatatgca gagctcggcg ctatgatccc tgaaacaggt ggtatctatg tttatttccg
      841 gaaaatgttt ggcgactttg ttgcctacct ctatggatgg gcagcattct ccgtgataaa
      901 tacaaccggt gtggccgcta tcgcatttgt atgtgcacaa tatgctgact actttctgca
      961 cctgccaaga tttgatgcgg caacagagca ggctgtaata tggcatttgc cttttatcgg
     1021 cgacctgcat ccactggaaa atttcggcgt aaaatcgctg gcaatagcaa tagttgcggg
     1081 gttaacctgg ctcaactacc gcagtgtaaa agcaggaagc tcattccagt tgatctcaac
     1141 aattgttaag ataggagtga tcgctgcttt ggtcattggt attttctttt ttggaaacgg
     1201 gtcagtggaa aattttttcc attcagaaaa tccaaagcag ggaagtgcat tgttagcagg
     1261 agttatcgct gcgcttaccg gggctttctt tgcctatgat ggatggatca atatcacttt
     1321 cattgccggg gagatcaagc agccacagcg aaatatcccg aagagtttat ttacaggagt
     1381 cattgcctgt atcattgtat atgtattggt gaaccaggct tacctctatg tattgccggt
     1441 agaaaaaatt gccacttcct ctttagtagc ttctgatgcc attgcaaagg ccattggaaa
     1501 tgctgccggt gcattggtgg cagcgatgat cgttatctgt acattaggag cagtaaacgg
     1561 taatttaatg gcaaccacaa gaattaccta tgcgatggga aaggacaaag tgtttctgcc
     1621 ctgggcaggt aaagaacata aaaaatttca gacaccgggt aatgcgttgt ggctgcatgg
     1681 tggctggata gccgttctta ttgtttccgg ttcatttgat atgctggcgg atatgatggt
     1741 atttatttcc tggatcgctt atggccttgg cgctgtcggt atttttatgc tgcgaaaaaa
     1801 aatgccggat gctgaacgac cttataagat atggctccac ccgtttgtca ctttactgtt
     1861 tatcactttt accgcattat tcctggtgat cactgtttat aatgatgtta caaattatat
     1921 tcatcaccgg cagcccgttg ttaattccct gctgggaata ttgataaccg ccctcggtat
     1981 tccgctttat ttttattacc ggaacaagaa ccggaaggat gacaccgcat taccggatca
     2041 acagggtagt acctttctgt aatacttttt caccggtacc gttatgggta tagctcagca
     2101 accaggcata cacaccggta tcctgcaaaa tgccattgat ccggccatcc catttttttg
     2161 tccagtcttt tgtataaaat actaattgcc ccatccggtt gtacacactg aattgcaggt
     2221 tgtctgcatt caatgcattc aacgggaaaa gataatcgtt gagcccgtct ccattggggg
     2281 taaatgcaga aggaacagca ataaaacaat tacttaatac ccggactgtt tgtcttgccg
     2341 tatcgcggca gttctgttgt gtattgccgg caataagagt gatggtatat aaagtttcat
     2401 tgccggtggc aacaaattga atggcggacg gcagcgcaag agtgctgaac tggccattac
     2461 caaattgcca ttgccattga tcaatattgc cactgctgtt attactgatc tttaatgtat
     2521 cgccgggaca gataattttt tgtacttcaa atgctgcctg caacacttcg ccggtcgtga
     2581 tgttttctgt agcagtatca ttgcagaccc cgttcgacac gatcagttgt atagtatact
     2641 gtccaccgcc cggaaaattt tgaactggat tttgctggtt gctggtaccg gcattgccaa
     2701 aggtccagtt ccagctgttt accccgttgc cgccgggatg ctgaaaagaa aaacgggtgg
     2761 cattacaggt ttcattcgca gcatacgtga aagaagcaga ggccgcaccc gccaccgtaa
     2821 aggatacgat accgggagct atcggcaaac cgcattcatc caataaagga ctgccattgc
     2881 tgccggtgct gagtgttacc tggtaagttc ctgcagtcgt tatgggtgat gccagttgca
     2941 aactaatgat atcactgacc gtgttgttag cgcaacgcaa tccccttgcc ccggaaatgg
     3001 ttaccgcctg cggtccggtc acaatgaatt ggcttccatc tgcggcaatg ctggaacaac
     3061 gtatcgggtt actgaatacc acttcaacag catccgggga acagttggcc gccaatgctt
     3121 cctgcacaac tgtttgctgt acatctgtta cagtaaaaga gatcgattca ttgaaaagga
     3181 tgaatttatt acagatatca ccgatggtat tgccatccgt gccattttgt aacagtaatg
     3241 cataagtgcc gttggctaat ggctgcgcca gtgtaatgat aatggaatcg gttccaagac
     3301 ctgaggtgca gctacccggt attgccccaa taacagcagc gccgttgctc aacctgaaat
     3361 cgcttccgtc tgcggcaatg gtattgcaaa cgatcttttt gttggtccgt aaaatgatat
     3421 gggtgccatc acaactggcc cttgcataat ccatgcgggg atcaatagca tcggtgatgc
     3481 tggccgtgcc acccgttaag gtaagctggt aactgccacc cgaaccgccc tggttacaga
     3541 ccatcagcag gtaggtatgt ccctgtatga tctgcggcat tttactgtag gtatttaaac
     3601 caccgccacc acagactatc gtgtctgttc ccaaatcggt ggcacctgtt tccccgggct
     3661 ctgatgacca gttacaggcc acaaacaggg aagcgtctgt gaaaatatca aatggattcc
     3721 tgttggtgat atcaaacagt tgccagtcat aattcatatt ggcatccggt acgatggtaa
     3781 atccgagagt gccgctgctg tagcaggaca tccggaaata attgggattg atattggtat
     3841 aagtaaaacc accgggacag gagaccggca ccagcgtatt gccacagtaa gtgggagtgt
     3901 tcatggtaaa aggatcatta ccacaaacag gaatggcaga tacaggagtt tgaccgggat
     3961 gtgtacagtt ctgctgtgcc tgcaacaggg gtgcgacagc caataaaagg aaaatgataa
     4021 ggcaatgctt tgccatcaga gatgaaatga aattgtaaat tacaaaaaca atatcgaaca
     4081 ggccattcta aaagcggcct tcccttttca ttactttcgt ataaatagtt taaaatgaga
     4141 acagccattg taaccggagc ttccggcaac ctgggccagg ccgttgtaca gaaatttttg
     4201 tcagaaggat atgccgtaga gggtacaggc tcattactga tggatgcacc gcattttcat
     4261 tctgtaactg ttgacctggt gaatgaagaa gcggctgctg aatgggtaca ggctgtaatc
     4321 aacaaacata agaccattga tgtggcggtg ctgacagtgg gtggttttgc tatggctgat
     4381 atagccggaa catcaacagc tatgatcggg cagcaatacc ggttgaattt tgaaaccgct
     4441 tataatgtgg caagaccagt ttttgtacag atgataaagc agggaacagg aactattttt
     4501 ttaataggtt caaaagcagg gatggaggct gcaaatagta aggggatgat cgcttacgga
     4561 ttgtccaaat cattattgtt ccggctggca gaactgatga atgaagaagc tgccggaacg
     4621 gcagtgaata ctatggtgat agttccctcc accattgata ccccacaaaa ccgtgcggcc
     4681 atgcccgctg ccgatttcag taaatggcaa acaccggagg ccattgcgga tgttattttt
     4741 tccaatacaa aacacaagga tagtaattca ccggtcattg aattatgaac ggtctcctgc
     4801 cgggatgatt ttctgctgta tcttgcagcc cattccaatt gaacgtgtat gaagtatatg
     4861 aaatggatcg gtttggcggc agcagttttg ctgatcgtat cctgttttat gccctgggta
     4921 attattgaat caagaaacat aaccgtgagc ggggtggatg ccaccggtac caactatggc
     4981 aagcccggtt atatccattt tgtgttgctt gcttttttta tagtgttcac cctggtgcca
     5041 aggctctggg ccaagcggat gaatttgctg gtggtggcct ttaatgcggc ctggatgctg
     5101 cgcaactttt tagtgatcgg tacctgcaaa gcaggggagt gccccgaacg ggagaccggc
     5161 ttctacctga tgataattgc ttcactcatt atgctgatct ccgcattatt tccggatata
     5221 aaaatgaaga acgatacagg taattgatgg ctcttgaaaa ggggatttta ccatgatttc
     5281 ataacacctg attttcggct gaactcctta tctttatttc ttccaatcct tccgaaatgc
     5341 cctttcatac ccaaactgaa actggcatta ttaaacggga accatgcgga agggattctt
     5401 catattacag ttattaattt ctcttgctta ttatacaaat gggcagactg ttgtaattag
     5461 ccaggtttat ggaggtggcg gaaattctgg cgcaacatat actcatgatt ttattgaaat
     5521 ttttaacgca actggctcac ctatctcttt aacaggctgg tctgttcagt ataattcagc
     5581 tgctgggaca ggcacttggc aggtaacaaa tttatcagga acattagccg ctggacaata
     5641 ttatttaatt caagaagcga tgggtagtgg aggatcagtt ccattaccga cacctgatgc
     5701 taccggaaca ataactatgg ctgctacaaa tgggaaagtt gctttactaa atacaacaac
     5761 agctttaact ggagacatgc cagttagtgc ctcagttatt gatttagtag gtttcggtac
     5821 tgcaaattat ttt
//
LOCUS       DAORTI010000177         5702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_16928_length_5702_cov_5.712591, whole genome shotgun sequence.
ACCESSION   DAORTI010000177 DAORTI010000000
VERSION     DAORTI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16928_length_5702_cov_5.712591"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLO70_15200"
     CDS             complement(<1..724)
                     /locus_tag="PLO70_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HQZ75866.1"
                     /translation="MIKLQFYLRFHTRFGQSIWISGNIDELGNNDPAKALSLDYLNDE
                     FWYGSIDIKRKETSKSVTYKYILKNENGELLFEWGNDRSIPLLKKEHNELQITDTWNH
                     AGEYENAFFTAPFNKVLLAQHFSKVKADSDKDFTHIFRVKAPLLKKNEAICLLGNGDK
                     LGDWSTIKPLLLEKNGDWWEIKLDLSDCNFPVAYKYGVYNTSEKSFTLYEDGDNRLLY
                     SDIVKKKLSILHDGFVHLPNNTW"
     gene            854..1378
                     /locus_tag="PLO70_15205"
     CDS             854..1378
                     /locus_tag="PLO70_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75867.1"
                     /translation="MIFRYKEWKQLFLLCLGLFAGTAFCMKWMEEDFIYNGSTFTIIG
                     LEISYPKVKVQEILSGLDYRVRTILSYHLYFDFAFMAGVYPGIAALCMMARDKVNNMG
                     VGKLLLVLACLQVVALGCDIAENSYLLTWLNKPVNGNEFQFYHIIVFIKWAIALAGAL
                     FAIPLAIRRRKIIV"
     gene            1432..1587
                     /locus_tag="PLO70_15210"
     CDS             1432..1587
                     /locus_tag="PLO70_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75868.1"
                     /translation="MEQTKKYRGIWWLVFFASTAALIFAIYTHWEWLTLILPFQTTAF
                     VKAMDIM"
     gene            1710..2120
                     /locus_tag="PLO70_15215"
     CDS             1710..2120
                     /locus_tag="PLO70_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HQZ75869.1"
                     /translation="MRLKMLILLSFLVIGTSVNNSFAALIVPAASVTTEPDPAKVKEA
                     VDAFKNLSKKEKKERAREVKKEIKAYKAAKKSGKDADTNTLLLVILAILLPPLAVYLH
                     EGEINNRFWISLLLTLLFWLPGVIYALIIILGDN"
     gene            complement(2182..2526)
                     /gene="rplT"
                     /locus_tag="PLO70_15220"
     CDS             complement(2182..2526)
                     /gene="rplT"
                     /locus_tag="PLO70_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQZ75870.1"
                     /translation="MPRSKNAVASKARRKKILKQAKGFYGKRKNVLTVAKNVVEKGMT
                     YMFVGRKLKKREYRSLWIARINAAVRAEGMTYSEFINKLQKKGIELDRKVLADLAMNN
                     PETFKNLVDSVK"
     gene            complement(2585..2782)
                     /gene="rpmI"
                     /locus_tag="PLO70_15225"
     CDS             complement(2585..2782)
                     /gene="rpmI"
                     /locus_tag="PLO70_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQZ75871.1"
                     /translation="MPKVKTNSSAKKRFKVTGTGKIMHQKAFKRHILTKKSKKRKRNM
                     RKDGTVAKSHIDFVKRLLRLK"
     gene            complement(3096..5639)
                     /locus_tag="PLO70_15230"
     CDS             complement(3096..5639)
                     /locus_tag="PLO70_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HQZ75872.1"
                     /translation="MQLSSRHFISLLFFTIISIAATAQQPGNTRPDTTRRPTNGPVTS
                     TGPKAYKDVITGKAKTDDGLFKVHKVEEKFFFEIPDTLLGRDILVVARLSKAPAGNSS
                     YAGDAINQKTIRFEKGPKDKIFMRLLSYLETGRDSTEGMYKNVLNSNLLAIAAAFDVK
                     AYSNDSASSVVDVTDYINGDNDIFFFSANTKRSMGLTGNPQSDKSYILNVKSFPSNIE
                     IKTVKTYAMTPPATSGSPSGFPGAGGSPGQPVTYELNTSLVILPKVPMQPRYFDDRVG
                     YFTTQTITDYDLNPQGVDRYRFIARYRLEPKDADIEKYKRGELVEPKKQIIYYIDPTT
                     PKKWVPYLIAGVSDWQIAFEKAGFKNAIVGKVAPTYEEDSTFSLEDARHSAIVYKPSD
                     IPNASGPNIHDPRSGEIIESHVNWYHNVMSLLRNWYMVQTAAVDPAARKMEFDDKLMG
                     ELIRFVSSHEIGHTLGLRHNFGSSSTVPVEKLRDKAWVEANGHTPSIMDYARFNYVAQ
                     PEDNISQIGLFPRIGDYDKWAIEWGYRWFPQARSTDEEIPVLNKWTIEKIEGNKRLWW
                     GDGEGNRGDPRSQTEDLSDNAMKASSYGIKNLQRIIIKLPEWTRQENKDFTGLGEMYG
                     QVTTQFGRFIGHVSNNVGGIQRTPKRVEQNGPVFEFTSKKTQKEAVAWIQANVFTTPL
                     WVIQKNITALTNLTPLSVIGGLQDRALGALISATTLSNLIRFEAEEPENAYTAVELLN
                     DVRKGVFTELGSRKPIDVYRRQLQKSLVERLIGLVNPATPQTVNVGGMTITISSGFSK
                     TSDGISLVKAQLKTLAAEIRTSLTGYGDAASKNHLQDLQDRIKLALEPK"
BASE COUNT         1597 a         1147 c         1068 g         1890 t
ORIGIN      
        1 tccatgtgtt attaggcaag tgaacaaagc catcatgaag aatacttaat ttctttttta
       61 caatgtctga atataagagc ctgttatccc catcttcata tagagtaaac gacttctcac
      121 tggtattata tactccgtat ttataggcta ctggaaaatt gcagtcggac aaatccagtt
      181 ttatttccca ccaatcacca ttcttttcca gtagtaaggg tttgatcgta gaccaatcgc
      241 ccagtttatc accattacca agtaaacata ttgcttcatt cttttttaat aaaggcgctt
      301 tgacccggaa tatatgtgtg aagtctttat ctgaatctgc ctttactttg gaaaaatgct
      361 gtgcaagcaa aaccttgtta aaaggggcgg taaaaaaagc attttcatat tcgccggcat
      421 gattccatgt atctgttatc tgcagttcat tatgttcttt tttgagtaaa ggaatactcc
      481 tgtcattacc ccattcgaat aataattcac cattttcgtt tttcagtata tacttgtatg
      541 taacactttt tgatgtttct tttcgcttga tgtcaataga gccataccag aattcatcat
      601 tcagataatc aagcgaaaga gctttagcag ggtcattatt gccgagttca tcgatattac
      661 cgctaatcca aatactctgc ccgaaacggg tatgaaaacg taagtaaaac tgtaatttta
      721 tcacaatcgt tagttctgag gttctaaatg caagttattg tgttttttcg acacattgtt
      781 tacgaaagta aaaaaaatag ctattgatat tgaattcagc cctattattt attaaaaaaa
      841 tcgacacaat cgtatgattt ttaggtataa agagtggaaa cagctttttt tattatgcct
      901 gggtcttttt gcaggcacag ctttttgtat gaaatggatg gaagaggatt ttatttacaa
      961 tggctcaact tttacaatta tcgggttaga gatatcatat ccgaaagtaa aggtccagga
     1021 aattttatcc ggcctagatt acagggtacg tacaatcctt tcctaccacc tgtattttga
     1081 ttttgctttt atggccggag tttaccctgg catcgctgcc ctttgtatga tggccaggga
     1141 taaggttaat aatatgggag taggaaaatt attgttggta cttgcatgtc ttcaggtggt
     1201 agcacttggt tgtgatatag cagagaacag ttatttactt acctggttaa ataaacctgt
     1261 taatggaaac gaatttcaat tctatcatat aatcgttttt attaagtggg ctattgcctt
     1321 agcaggggct ttgttcgcaa ttcccctggc aatccgccga agaaaaatta ttgtctaaaa
     1381 ctattttttt gatttagtgc tgccacctta tttttgccta aattttttaa aatggagcaa
     1441 acaaaaaagt atagaggtat ctggtggctg gtattttttg catctaccgc tgcacttata
     1501 ttcgctatat acacccattg ggaatggctg acacttattc tcccctttca aaccacggcc
     1561 tttgtaaagg caatggatat catgtaataa ttaacacctg ttgatattta tattttaata
     1621 atcccgccat gtgcggggtt attttttacc ttccggcaca ttattggtga caattaacca
     1681 ttatttttaa accagctaaa taaaatttta tgagactaaa aatgttgatt ttgctttctt
     1741 tcctagttat cggaacttct gtaaataatt cttttgctgc acttattgta cctgctgcat
     1801 ctgtaacaac tgagcctgat cctgcaaaag taaaagaggc ggttgatgcc tttaaaaatt
     1861 tatctaaaaa ggaaaaaaag gaaagggcca gagaagttaa aaaagaaatc aaagcctaca
     1921 aggcggcaaa gaaatccgga aaagatgccg acacaaatac actattactg gtgatactgg
     1981 caatcttatt acctccactg gctgtatatc ttcatgaggg agaaataaac aacaggttct
     2041 ggataagttt gttgcttaca ttactattct ggctccctgg tgtgatctat gcgttgataa
     2101 tcattttggg tgacaactaa tcaatttact ctataaaaaa aagccccgta taccggggct
     2161 tttcattttg gtctgtataa attattttac agaatcaacc aggtttttaa atgtttccgg
     2221 gttattcatc gcaaggtcag ccagtacttt acgatccagt tctatacctt ttttctgcag
     2281 cttgttgata aactcagaat aggtcattcc ttcggcacga acagcggcat tgatacgggc
     2341 aatccacaaa gagcggtatt ctcttttctt taatttacgg cctacaaaca tgtaggtcat
     2401 acctttttct actacgtttt ttgctaccgt taatacattt ttacgtttgc catagaaacc
     2461 tttggcttgc tttaatattt ttttgcggcg ggctttggag gccacagcat tttttgaacg
     2521 aggcatattt ttaagtttga ggtcagctat acatagtacg cactgacagc gttatcaaat
     2581 ttgtttactt caggcgcaac agacgtttta caaaatcaat atgtgatttt gcaactgttc
     2641 cgtctttccg catgttacgt ttacgttttt ttgatttctt tgtcaggatg tgacgcttga
     2701 aagctttctg atgcatgatc tttcctgtgc cggtcacttt aaagcgtttt ttggcactgg
     2761 agtttgtttt tactttaggc atgttaccca gtattttata ttacaccctt gaggcattct
     2821 ccacagggag acctttgtcg aacctcttca ggcaattaat gtccttaatt agtaaggact
     2881 ttggaagaac taccgttttt ttgaacgggg tgcaaagata ggattgatat agcatgtggc
     2941 aaagaagtct taaaaaaaga ttggtttatc ctgttattac tacttcaaag aaaatatatc
     3001 cgatttgaat cgtaatgtaa ttaaaatcaa atagttgtaa aatataaaag aggttatgct
     3061 tttacataac ctcttttata ccctaaactg atcagttatt ttggctctaa agcaagtttt
     3121 atcctgtcct gcaaatcttg caaatgattt ttggaagccg catcaccata cccagtcaac
     3181 gatgtgcgga tctcagctgc cagtgttttt aattgcgctt taacaagaga aatgccatca
     3241 ctggttttag aaaatcctga gctaattgtt atcgtcatcc ccccaacatt tacggtttgt
     3301 ggtgttgccg gattaaccaa ccctatcaat ctttcaacaa gcgacttttg cagttgacga
     3361 cggtaaacat ctattggttt ccgggaccct aattctgtaa atactccttt gcgtacatca
     3421 ttcagtaatt ctactgctgt ataggcattt tcaggttctt ctgcttcaaa acggatcaaa
     3481 ttagataagg tggttgcact gatcaaagca cctaaggctc tgtcctgtaa acctccgata
     3541 acagaaaggg gagtcaggtt tgtaagcgct gtaatattct tctgaataac ccaaagtggt
     3601 gtagtaaaaa cattggcttg tatccaggct actgcctcct tctgtgtttt tttagaggtg
     3661 aattcaaaaa cgggtccgtt ttgttccacc ctttttggtg tccgctggat acccccaaca
     3721 ttattactga catgcccgat aaatcttccg aattgggttg ttacttgtcc atacatttca
     3781 cccaaacctg taaagtcttt attttcctgc ctggtccatt caggcagctt tataataatt
     3841 ctttgaaggt ttttaatccc gtatgaactt gccttcatag cattatcact aaggtcttca
     3901 gtctggctgc ggggatcgcc tcgattgccc tcgccatcac cccaccacaa tcttttattc
     3961 ccctcaattt tttcaattgt ccatttattc aaaacaggaa tctcttcatc tgttgacctg
     4021 gcttggggaa accaacgata accccattca atagcccatt tatcataatc cccgatgcgt
     4081 ggaaataatc ctatttgtga aatattgtct tccggttgtg ccacataatt aaaacgggca
     4141 taatccataa tggaaggtgt gtggccattt gcttctaccc atgctttatc tcttaatttt
     4201 tccaccggaa cagtggacga agaaccaaaa ttatgacgaa gccccaatgt atgcccaatt
     4261 tcatgactgc taacgaaccg gatcagttct cccattagtt tatcatcaaa ctccattttt
     4321 ctggcagccg ggtctacggc tgcggtctgt accatatacc agtttctcag caagctcatt
     4381 acattatgat accagtttac atggctttct ataatttctc cgctgcgtgg atcatgaatg
     4441 tttggaccac ttgcattggg gatgtcagaa ggtttataaa cgatagcact gtgacgggca
     4501 tcctcaaggc tgaaagtgct atcctcttca taggtcggcg ctacctttcc tacaattgca
     4561 ttcttgaatc cggctttttc aaacgctatc tgccaatcgc ttaccccggc aatcagataa
     4621 ggcacccatt ttttgggagt tgtgggatcg atataataaa taatctgctt cttaggctcc
     4681 actaattccc ctcttttata cttttctata tccgcatctt tcggttcgag ccggtaacgg
     4741 gcaatgaacc tgtacctgtc aacaccctgt ggattcagat catagtcagt aattgtttga
     4801 gtagtaaaat aaccgaccct gtcatcaaag tagcggggtt gcatcggcac tttaggtaaa
     4861 atgaccaggg atgtattcaa ttcgtaagta accggttgtc caggagaacc accagccccc
     4921 ggaaaacctg aaggtgaacc cgatgtggca ggaggagtca tggcataagt tttcactgtc
     4981 tttatttcaa tattggatgg aaatgatttt acattgagaa tataagactt gtcactttgt
     5041 ggattacctg tcaggcccat agaacgtttg gtattggccg aaaaaaagaa aatatcattg
     5101 tcaccattta tataatcagt tacatctacg acactactgg ccgaatcatt cgaatacgct
     5161 tttacatcaa aagcggctgc tatagccagc aagttactgt ttaaaacatt tttatacatt
     5221 ccctccgtac tgtcccggcc tgtttcaaga taagacagca gtcgcatgaa tattttatct
     5281 tttggacctt tttcaaaacg aattgttttt tggttgatag catcacctgc ataactactg
     5341 tttcctgcag gagctttgga caatcttgct acaaccaata tgtccctgcc caacagcgta
     5401 tcaggaattt cgaaaaaaaa cttctcctct actttgtgta ctttaaaaag accatcatcc
     5461 gtctttgctt tgccagtaat aacatcttta taagctttgg gtcccgtcga tgttaccggc
     5521 ccgttggttg gtcttcgtgt agtatccggc cgggtgttac cgggttgttg tgcagtggca
     5581 gcaattgaaa tgatagtgaa aaacaaaaga gaaataaagt ggcgagatga taattgcata
     5641 aatatcaatt ttaagtggct caagatactg aaaggagcgg atcagtaacg ttgaatgtta
     5701 at
//
LOCUS       DAORTI010000178         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_16969_length_5695_cov_4.674113, whole genome shotgun sequence.
ACCESSION   DAORTI010000178 DAORTI010000000
VERSION     DAORTI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16969_length_5695_cov_4.674113"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLO70_15235"
     CDS             complement(<1..872)
                     /locus_tag="PLO70_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75873.1"
                     /translation="MKRILLTMLLTLTAGMIYSQTTYYWVGGTASANSITIGTNWNTS
                     LDGLGSPRPSSTGATDLLIFDGTNVGGTTPATGPVTVLVNGSITCAQIKLINNADISW
                     IRASSGTSTITISGEAGEDFLVESGSKLSFVSTVGSLRVAMGAANTGRVSGTLIMNTI
                     WQARFDNTTTGSPGSFIFTNGAVFRTNITSGSSSYAFGSGTQSAEKWVVFEDGAHLYY
                     DGGYSPMGGTSTFSAIDFKPGSFWHHRANNELGSFLNRKSFGNIIVENNATLTADGPV
                     YRINNLTVETGATF"
     gene            complement(906..2198)
                     /locus_tag="PLO70_15240"
     CDS             complement(906..2198)
                     /locus_tag="PLO70_15240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ75874.1"
                     /translation="MKIKGLRWYIIGLIGLATIINYIDRTAINILWPYIHKDFGIAEA
                     DSKDSLKLITTFFMIAYALGQTLTGKLMDAIGTRLGFLVSIVGWSISIALHAVAKTLG
                     SFNIFRFFLGLSEAGNWPGAAKSNAEWFPAKERAIAQGIFGAGASLGSVVAAPFIAYL
                     YIAFGWKMTFVGIGVLGLLWIIPWLIINKNTPDKHPWITKEEQDHILQSATNKATAPV
                     ATTVFSWKQLLGFKNTWAVITSRFFIDPVWWMFVTWLPFFLKENFGFDIKQVGAFAWV
                     PFLFAAIGGLAGGFFSSIQIKNGIAAVKARKNAITIGSVIMLLSLGSIAYYLDQLKEL
                     PGVAIALISSTLFGFQFLINNLQTLPGDYFHGKNVGTVAGMGGTSAVAGTLIVTWLVP
                     VLTKTSYTSFFLLAALLVPLAWICIMFIVSKRKELEDK"
     gene            complement(2205..3230)
                     /locus_tag="PLO70_15245"
     CDS             complement(2205..3230)
                     /locus_tag="PLO70_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75875.1"
                     /translation="MLKFLHIITWFLLMSCNKGNTPSQTTPVVPPNPVSSRVVNVSTA
                     AQLSAALLNALPGDEIVMANGIYKGKFVIGSAVSGTAAKPVLLRGSRNVILDAENVNT
                     GYVLHLQASYWKVKGFTLTNGLKGLMVDGARYCVIDSIYVHTIGEEGIHLRKFSSQNT
                     IQNSEVSNIGLKTPDYGEGIYIGSAKSNWATYTNGVPDLCDSNKIFNNKLGPGITAEC
                     IDIKEGTTGGIIRGNNFDATGITGANSGDSWIDVKGNNYLIEENTGYNPGGSIFKDGY
                     QVHVAYAGWGNYNVFKNNECSVNAAGYGFNIQLSGSNGTSTGNKVYTDNTVSGALSGT
                     ANIPLSN"
     gene            complement(3243..3596)
                     /locus_tag="PLO70_15250"
     CDS             complement(3243..3596)
                     /locus_tag="PLO70_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ75876.1"
                     /translation="MGTTESVKVFIENAEIPWQEVDKGVKRKIMAYDERLMVVKVVFD
                     KGGVGSLHKHPHTQITHVESGLFEVEIGEEKKTLKGGDAFYIPPNVMHGAVCLESGIL
                     IDVFSPMREDFIENK"
     gene            complement(3642..>5695)
                     /locus_tag="PLO70_15255"
     CDS             complement(3642..>5695)
                     /locus_tag="PLO70_15255"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HQZ75877.1"
                     /translation="TRSYTDIKKEVDQWIGKEVDVPFPKDPAGGYTHDKHKYNYMLMF
                     NSGILYNLTGDVKYAKLVKNVLLKYAVLNPTLKNHPQATSSSPGRIFWQALNDANWLV
                     YTGMAYDLVYNSLTVAERKKIEDGAFKPEVDFITKDLQSWFDLIHNHGVWACAGVGIV
                     GIASGSKDYVDMALYGTKKDGKSGFIALMDGLFSPDGFYAEGPYYTRYAILPYYLFAT
                     ALNNSKSKLKVFEYRDRILQKALLAALQQTNLDGTFFALNDALKEKDYTSNELVTAVD
                     VAWDIYGKDNGLLVVAKKQNRVLLNRGGISIAAAIAAGKNVPEFFPYRTVEYTDGVKG
                     DQGGVSVLRNGKGNDLTSLIYKYTSHGLSHGHYDKLNYNLYDKGKEILTDYGAVRFIG
                     VEQKYGGRYLPETKGYAAQTIAHNTIVVDEKSHFDGKESVAEKFHAEKLFSSISNPAV
                     QVVAAKVDQAYKGVNLQRTVYLLELPGNQKILVDIFNAASKDEHQYDLPFHYNGHLIS
                     TSFKYSPYTGMQEPLGKKNGYQYLWKEADAMVADTVVQLTFLNERTYYSISSLVKGQA
                     QVFFTRSGANDPNFNLRHEPALIIRKKGIDQSFVSVTEIHGKFDPVIEFSTNSYSAVK
                     NIELLQQDEQFSIVEILLNNKKLIVAQCNMDIDSKAVHTSAGYSWTGPFAIYYDGKKM
                     N"
BASE COUNT         1685 a         1351 c          953 g         1706 t
ORIGIN      
        1 ttaaatgtgg cacctgtctc tacagtaaga ttattgatcc tgtaaacagg gccatctgca
       61 gtcagtgttg cattattctc tacgatgata tttccaaaac tcttacgatt taaaaaagaa
      121 cctaattcgt tatttgcccg gtgatgccag aagcttcccg gtttgaaatc aatggctgaa
      181 aaagtggaag ttccgcccat tggactatac ccaccatcat aataaaggtg agctccatct
      241 tcaaatacca cccatttctc agccgattgt gttccgcttc caaacgcata agaagaggat
      301 ccggaggtaa tatttgtcct gaaaaccgca ccattagtaa atatgaaaga ccccggacta
      361 ccagttgttg tattatcaaa tctagcctgc catattgtat tcataattag cgtcccgctc
      421 accctgcctg tattagcagc tcccattgca acacgaaggc tccctacagt agaaacaaat
      481 gaaagcttgg atccggattc taccagaaag tcttctccgg cttcaccact gatagtaata
      541 gtgcttgttc cggaagatgc ccgtatccaa ctaatatcag cattgttaat aagcttaatc
      601 tgtgcacagg taatgctgcc atttactaat accgtaaccg gcccggtagc cggtgttgta
      661 ccgcctacat tggtaccatc aaatataagt aggtctgttg cgcctgtaga tgatggccgc
      721 ggactaccta atccatccag ggatgtattc cagttagtac caatagttat actattagca
      781 gatgcagtac cccctaccca gtaataagta gtttgactgt aaatcattcc tgctgtcagt
      841 gtcagcaaca tcgtcaataa aattcttttc atattgcagc ttttaaatcg tttagttgaa
      901 ttacattatt tgtcctctaa ttcttttctt tttgatacaa taaacatgat acatatccag
      961 gccagcggaa ccagcaaggc cgccagcaga aaaaaagaag tatagcttgt ttttgtcaat
     1021 acaggtacca accaggtaac gatcaatgtt ccggctacag cagatgttcc tcccatacct
     1081 gctacagttc ctacattctt tccatggaaa taatcacccg gcagcgtctg aagattatta
     1141 atcaggaact gaaatccaaa aagtgttgaa ctgatcaatg cgatagcaac acctggcagt
     1201 tcttttagct gatctaaata ataagctata ctacccagcg atagcagcat aataacactt
     1261 ccgattgtaa ttgcattttt tcttgctttg actgcagcta taccattttt gatttgtatg
     1321 gaagagaaaa atccacctgc taaaccacca atagccgcaa ataagaaagg aacccaggca
     1381 aaagccccca cctgcttaat atcaaaacca aaattttctt ttaagaagaa aggcagccag
     1441 gtaacgaaca tccaccatac cggatcgata aaaaatcttg aagtaataac agcccatgta
     1501 tttttgaaac ccagtaattg tttccatgag aacactgtcg ttgccaccgg cgctgttgct
     1561 ttattggtag ccgattgcaa tatatgatcc tgctcttcct tagtgatcca tggatgttta
     1621 tccggggtat ttttattaat gatcagccag ggaatgatcc ataacaatcc aagtaccccg
     1681 atgccaacaa aagtcatctt ccagccaaaa gcgatataca gatatgcaat aaaaggcgct
     1741 gctaccacag aacccaatga agcacctgca ccaaaaatac cttgcgcaat agctctttct
     1801 ttggccggga accattctgc attactcttg gcagcaccag gccagttgcc agcttcactc
     1861 aaccccaaaa agaaacggaa aatattaaat gaacccaggg ttttggctac tgcgtgaagt
     1921 gcaatggaga tactccatcc aacaatagaa acaagaaacc ccaggcgggt gccaatagca
     1981 tccatcagtt ttccggttaa cgtttgaccc aacgcatatg cgatcataaa aaaagtggtg
     2041 atcagcttca acgaatcctt actgtctgct tcagctattc caaaatcttt atggatataa
     2101 ggccagagta tattaatggc agtccggtca atataattaa tgatcgttgc cagcccgatg
     2161 agcccaataa tataccagcg tagccctttt atcttcataa tattttaatt actaagcgga
     2221 atattggcgg taccgctcaa tgcaccgctt acggtattat cagtatatac tttgtttccg
     2281 gttgacgttc cattactgcc actcaactgg atattaaatc catacccggc agcattaacg
     2341 gaacactcat tattcttaaa tacattataa ttaccccacc ctgcatatgc tacatgtacc
     2401 tggtagccat ctttaaaaat ggaaccaccc gggttataac ccgtattttc ttcaatcagg
     2461 taattatttc cttttacatc gatccagcta tcaccactat tggcacccgt aataccagtg
     2521 gcatcaaaat tgtttccccg gatgatacct ccagttgttc cttctttaat atcgatacat
     2581 tctgccgtta tgccgggacc taatttatta ttgaaaattt tattagaatc acagagatcc
     2641 ggaaccccgt tggtatatgt agcccaatta ctttttgcag aaccgatata aataccttca
     2701 ccgtaatcag gtgttttcaa tccaatattg ctgacctcag aattctgaat ggtattttgt
     2761 gaactgaatt ttcgcagatg tatcccttct tcacctatag tatgaacata aatactatca
     2821 attacacaat acctggcccc atccaccatt aatcctttca agccattggt cagagtaaaa
     2881 ccttttactt tccaataact ggcctgcaaa tgcaaaacat agcctgtatt gacattttca
     2941 gcatcgagga tcacattacg gctacccctg agtaaaaccg gctttgcggc agtaccgcta
     3001 acagcagatc cgataacaaa ttttcctttg taaataccat tcgccattac gatctcatcg
     3061 ccaggcagtg cattcagtaa tgcggcactt agttgagctg ctgttgaaac attcacaact
     3121 ctgctgctaa cagggttcgg tggtactact ggtgttgttt gcgatggcgt attgccttta
     3181 ttgcaactca tcaacagaaa ccaagtaata atatgtagga atttcagcat cctttttttg
     3241 cattatttat tctcaataaa atcttcccgc ataggactga acacatcaat taatatcccg
     3301 ctttccaaac aaacagcacc atgcattaca ttaggcggga tgtagaaagc atcacctcct
     3361 tttaaagttt ttttctcttc cccaatttct acttcaaata aaccgctttc aacatgggtg
     3421 atctgggtat gaggatgctt atgcaatgaa ccaactcccc ccttatcaaa caccactttt
     3481 accaccatca gcctttcatc ataagccata atctttcttt taacaccctt gtctacttcc
     3541 tgccatggta tttcagcatt ttctataaat acttttaccg attcagttgt tcccatttct
     3601 ttctttttat atgtttttac aatatgttct ccgctatttt atcaattcat ctttttcccg
     3661 tcataataaa tggcaaaagg gcctgtccac gaatacccgg ctgacgtatg tacagccttt
     3721 gaatctatat ccatattaca ctgtgctaca attaatttct tattattcag caatatttct
     3781 acaattgaaa actgttcatc ctgctgcaac aattcaatat tttttacagc agaataggaa
     3841 ttagtagaaa attcaatgac cggatcaaat tttccatgta tctccgttac gcttacaaat
     3901 gactggtcta ttcccttttt acgaatgatc aacgcaggtt catgtcggag attgaaattg
     3961 gggtcgttag ccccggaacg ggtaaaaaat acctgtgcct gtccttttac cagggatgaa
     4021 atactgtaat aagttctttc attcaggaat gtaagctgaa ctacagtatc ggcgaccatt
     4081 gcatctgcct ctttccataa gtattggtac ccattcttct tacccagcgg ctcctgcatg
     4141 cctgtatacg ggctatattt aaatgaagta ctgataagat gaccattata atgaaatgga
     4201 agatcatact ggtgttcatc ttttgaagca gcattaaaaa tatcaaccag aattttctga
     4261 ttacccggta attccagaag gtaaactgtc cgttgcaggt taactccttt ataagcctga
     4321 tcaacttttg ctgccacgac ttgtacagcc ggattgctga tactgctgaa tagtttttca
     4381 gcatgaaatt tttcagctac actttcttta ccatcgaaat ggcttttttc atccactact
     4441 attgtattat gagcgatggt ctgcgcagca tatcctttcg tttcaggcag gtatcttccg
     4501 ccatactttt gctcaacacc tataaaacgg acagcaccat aatcagtaag tatctctttg
     4561 cctttatcat ataaattata attcaattta tcataatgac catgcgaaag accatgagaa
     4621 gtatatttat atatcagtga tgtaagatca ttgcctttac cattccgcaa cacactgact
     4681 cccccctgat cccctttaac accatctgtg tactccactg tccggtaagg aaagaattcg
     4741 ggaacatttt ttccggctgc aatagccgct gcaattgaga tacctccccg gttcaacaac
     4801 acccggttct gtttcttagc aactaccagt aagccattat cttttccata aatatcccag
     4861 gctacatcaa cggcagtcac cagctcatta ctggtatagt ccttttcttt caaagcatcg
     4921 ttcaaagcaa aaaaagttcc gtcaagattc gtttgctgca gcgccgccaa taatgctttt
     4981 tgtaaaatgc ggtcacggta ctcaaaaact ttcaacttag attttgaatt attaagcgct
     5041 gtagcaaata aataataagg aagaatagca taacgggtat aataaggacc ttctgcataa
     5101 aagccatccg gtgaaaacaa accatccatc aatgcaatga aaccactctt gccatctttt
     5161 ttggtaccat acaatgccat gtcaacataa tccttgctgc cggatgcaat cccaacaatt
     5221 cccactccgg cgcaagccca tacaccatga ttatgtatga ggtcaaacca ggactgaagg
     5281 tctttggtta taaaatcaac ttcaggttta aaagctccat cttcaatttt ttttctctct
     5341 gcgacagtta gtgaattata tacgagatca tacgccatac cggtataaac caaccagttt
     5401 gcatcattca atgcctgcca gaaaatacga cccggtgaac tactcgtagc ctgcggatga
     5461 ttttttaacg taggatttaa cacagcatat ttcagcagga cattcttaac cagttttgca
     5521 tatttaacat ccccggtaag attatataat atgccgctgt taaacatcag catataatta
     5581 tacttatgct tatcatgcgt ataacctccg gccgggtctt tcggaaatgg cacatccact
     5641 tccttcccga tccattgatc cacctctttc tttatatcag tataagaccg ggtaa
//
LOCUS       DAORTI010000179         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17001_length_5688_cov_8.515356, whole genome shotgun sequence.
ACCESSION   DAORTI010000179 DAORTI010000000
VERSION     DAORTI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17001_length_5688_cov_8.515356"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1230)
                     /gene="gldN"
                     /locus_tag="PLO70_15260"
     CDS             complement(202..1230)
                     /gene="gldN"
                     /locus_tag="PLO70_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HQZ75878.1"
                     /translation="MKMNFLKFGICLAVAALLTVDANAQRTPKKRKDNTPPTTTNPTE
                     GNEQQQNNNNQPPAGYDPNANLPVTYDTTSNDLTPKKSLRQDNAFDKSNLTQRTPLPY
                     EHLRWDDALYAEKVWRELDLREKMNKIFMYEAVDDNGSQIFVNMLMKAVQSGEVTAFT
                     DDRFTEPKSIADVQQLTSGRLDTNYVFDTKQIDKVVGLNVSRASFDAKSITKIRLKEE
                     WVFDRESSRMFVRILGIGLLKTEYFPNTTKERGTSNLFWVYYPDLRPMMAKAEVYNPK
                     NMGQSRMTWEELFESRMFSSYIVKSTLDNPSNKMIRNYINDPILRLLEGDNIKEKIFN
                     FEQDLWSY"
     gene            complement(1237..2748)
                     /gene="gldM"
                     /locus_tag="PLO70_15265"
     CDS             complement(1237..2748)
                     /gene="gldM"
                     /locus_tag="PLO70_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HQZ75879.1"
                     /translation="MSLPKEPRQKMINIMYLVLTALLAINISAEILNAFKTINESLEK
                     TNNAVKISSDRIFASLTDKMSDPTTKVKAEIWQPRAKQVMDYSATAYNFIENLKKELI
                     AASSKGGTFKEDEQNATTRLLVKEGKGKELFKILEAYKKNVLGINDTIQKEFANSLQV
                     DLTPPKSQNSKKKTWEEAYFYMAPAVGALSILSKFQNDIKTSENKVVTFCHEQVGKVE
                     LRFDSFEAIVGQNSKYFMPGQEIEITAGLGAFSKTKLPVVTVNGSGVTLNEKGQAIYK
                     AAAGGVGQHTIQVKVAFTDQDGNPQERTIPVEYTVGSANASIALDKMNVLYIGVDNPV
                     TIAASGGGDDKVQATISNGSLTRVGPGKYIARVNTVNDNTLITVTVEGKVAGASQFRV
                     RTIPEAQAYVGGKPSGENITAGAFKAQGGVGAGIKNFPFELEYDVVSFTFTCDTDDDI
                     VILPNQGANFSQAVRNAMNQHVQAGRTVTIDEIRVKGPDGRTTKAPSLVYYIK"
     gene            complement(2828..3601)
                     /gene="gldL"
                     /locus_tag="PLO70_15270"
     CDS             complement(2828..3601)
                     /gene="gldL"
                     /locus_tag="PLO70_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HQZ75880.1"
                     /translation="MAAAIPSKVSKWVDVGVSFGAALVIWGALRKITHQPDADTWLWI
                     GLTTEALIFTLYGILYAVYPPIKDASHDEEQFSVAGAKSRAAFGAMDKMLEQADITPD
                     TMKRLGDGFKQLNTTVSGMNGIGDTINATADFTTKTREVTGQLEKVKDAYTTAASSVG
                     AFNTATEGAKVFHEQIQVLTKNLSSLNTIYELELQESNNHLKALNAFYGKLSETSNSM
                     LNSAEDAKRVQEQIGNLATNLGRLNNIYGNMLTAMQGRS"
     gene            complement(3709..5016)
                     /locus_tag="PLO70_15275"
     CDS             complement(3709..5016)
                     /locus_tag="PLO70_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQZ75881.1"
                     /translation="MRNLYYTLLAASCMTMFASCGILGGKKNKGGSLPNDGQVHGVAP
                     GSKYSLPKPPGMVYVPQGTFHMGPSDEDPAYAFSARNRSVSISGFWMDATEITNNEYR
                     QFVYWVRDSVVARKMGYVKAGQDGNEYVDWAKMKTMKWGDPKVMEQLSSTDIILPPDD
                     RVFGKKEIDPSKIVFHSETFDLKEAAKRENAGKPRSTFIVKKDVGIYPDTLAWIRDFS
                     YSYNEPMTKRYFSHPAFGNYPVVGVNWKQATAFCEWRTHFLNAYLDSKKRTQESDFRL
                     PTEAQWEYAARGGRSQSMFPWGNYYLRNKKGCLMANFKPGRGNYPEDGGFYTVRADAY
                     WPNDYGLYCMSGNVAEWTSSIYYEGRNNFQHDMNPDVRWNAKDDDPPLMKRKIIRGGS
                     WKDVGYYLQTGTSTYEYQDSTKSYIGFRCVIDLPAAPQKGRRK"
     gene            complement(5170..>5688)
                     /locus_tag="PLO70_15280"
     CDS             complement(5170..>5688)
                     /locus_tag="PLO70_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQZ75882.1"
                     /translation="IENFSDEETPFFFYTPAGQQDVIIRKKEVYDGAVPSGNSVMAYN
                     LYHLSVLFGKREWGKRSLDMILSLNTAIIRYPTSFGNWACLLQEIIAGTSEIAVVGKN
                     LYNLHTEILQQYIPHRVLMTAEKGDNSFPLLAGKMTADKPTIYLCRSYTCLNPVFSAK
                     ELISLIDKGQKQ"
BASE COUNT         1589 a         1228 c         1056 g         1815 t
ORIGIN      
        1 gggccggaat agaaattcag ccccgttttt aaaatccaat atcctatgaa aaaccaagac
       61 gaaagtacaa cttttatcca attatcctat aggactttta cgattttcaa aacttttttt
      121 acattataat tgaattagtt aggaaacaaa aaagcccctt aatggggctt tttaattaat
      181 gtttaaacaa acaataaaat attagtaaga ccagagatcc tgttcaaaat tgaatatctt
      241 ttctttgata ttatctcctt caagtagtct taagatcgga tcgttgatat aattcctgat
      301 catcttattg cttgggttat caagtgttga tttcacaata taactgctga acatccggct
      361 ttcaaataat tcttcccatg tcattctgct ttgacccatg ttctttggat tatatacttc
      421 tgcctttgcc atcataggtc taaggtccgg gtaataaacc caaaacaggt tagatgtacc
      481 tctttctttg gtagtattag ggaaatactc tgtttttaaa agtccgatac caagaatacg
      541 tacaaacatt ctgcttgatt ccctgtcaaa aacccattct tccttcaggc ggatttttgt
      601 aatacttttt gcatcaaaac tggcccttga cacatttagt ccaactactt tatcaatttg
      661 ctttgtatca aaaacatagt tggtatccaa ccgtcctgaa gtcagctgtt gaacatcagc
      721 aattgactta ggttcggtaa acctgtcatc agtaaatgca gttacttcac cagattgcac
      781 tgccttcata agcatattta caaaaatctg gcttccgtta tcatctactg cttcatacat
      841 gaagatctta ttcatttttt cccgcaggtc cagctctctc catacctttt cggcataaag
      901 ggcatcatcc cagcgtaggt gttcataggg taatggtgta cgctgagtta gattactctt
      961 atcaaaggca ttatcttgtc tcaatgactt tttgggagtc agatcattac tggtagtatc
     1021 gtatgtaacc ggaagattag cattggggtc ataaccagcg gggggctgat tattgttatt
     1081 ctgctgttgc tcatttcctt ccgtcgggtt tgttgttgtt ggcggtgtat tgtctttcct
     1141 tttcttcggt gttctttgcg cattggcatc gaccgttaaa agggctgcaa cagccaaaca
     1201 tattccaaac tttaaaaaat tcattttcat atcacactat ttaatataat aaacgagtga
     1261 aggtgccttt gtagtacgtc cgtccggtcc ttttaccctt atttcatcaa tggttactgt
     1321 tctgccagcc tgaacatgct gattcatagc atttcttacg gcctgggaaa aattagctcc
     1381 ctggttaggc agaattacta tatcatcatc tgtatcacaa gtaaaggtga aactaactac
     1441 atcatattcc agttcaaaag ggaagttttt aataccagca cctacaccac cctgagcttt
     1501 gaaggcacca gctgtgatat tctcaccact gggctttcca cctacatagg cttgcgcttc
     1561 aggtatggta cgaaccctga attgagaagc accagcgact ttaccttcca cagtaacagt
     1621 tattaatgta ttatcattaa ctgtatttac ccgtgcaata tattttccgg gtccaaccct
     1681 ggttagggat ccattgctga ttgttgcctg cactttatca tcaccaccac cgcttgcagc
     1741 aatagtcaca gggttatcta ccccaatata aagtacattc attttatcaa gtgcaataga
     1801 agcattggct gagcccacag tatattcaac cggaatggtt ctttcctggg gattgccatc
     1861 ctgatcagta aatgcaacct taacctgtat cgtatgttgg ccaactcctc cggctgcagc
     1921 tttatagatg gcctgccctt tttcatttag agtaactcct gatccattta cagttacaac
     1981 gggaagtttg gttttactga aagcacctaa gcctgcagta atctctatct cctgaccggg
     2041 cataaaatac tttgaattct gtccaacaat tgcttcaaaa ctatcaaacc ggagttccac
     2101 cttacccacc tgctcatgac agaaggtaac tactttgttt tctgatgttt taatatcatt
     2161 ctgaaactta ctgagaatac ttaatgctcc tacagcaggt gccatgtaga agtaagcttc
     2221 ttcccatgtt tttttcttgg aattctgtga tttgggaggt gtaagatcaa cctgcaaaga
     2281 attagcaaat tccttttgga tagtatcatt tatacccaac acattttttt tatacgcttc
     2341 aagaatttta aaaagctctt ttcctttacc ctcttttaca agaagtctgg tagtagcatt
     2401 ttgctcatcc tctttaaatg tgccgccttt ggaagaggct gctataagct cttttttcag
     2461 gttctcaata aaattataag cagtagcaga ataatccatt acctgctttg ccctgggttg
     2521 ccagatctct gcctttactt tagtagtggg atcagacatt ttatctgtca atgaagcaaa
     2581 aatccggtct gaggatattt ttactgcatt attggttttc tccaggcttt catttatggt
     2641 tttaaaggca ttgaggatct cagccgaaat gttaatcgcc aaaagggcag ttaacaccag
     2701 gtacatgata ttgatcatct tctgccgggg ttctttaggt aaagacatga agattcgtgg
     2761 tttttaattt agaaatagtg acttgttaga atcacggata cggattcgtt ctgattatta
     2821 gtttgtttta gctgcgaccc tgcatggctg ttaacatgtt accataaata ttattcaggc
     2881 ggcccaggtt agttgccagg tttcctatct gttcctgtac tctttttgca tcctcagcac
     2941 tgttcagcat tgaatttgaa gtttcagaca gtttaccata gaaagcgttc aatgctttca
     3001 aatggttatt actttcctgc aactccagtt catagatcgt attaagagaa gataaatttt
     3061 tggtcagaac ctgaatttgc tcatgaaata ctttagcacc ttcagttgct gtattaaatg
     3121 ctccaacgga ggaagcagct gtggtatacg catccttaac tttctctaat tggccggtaa
     3181 cttctcttgt tttagtagtg aagtcagcag tagcattaat ggtatcacca ataccattca
     3241 tcccggaaac agtagtgttc aattgtttga agccatcacc gagacgtttc attgtatcag
     3301 gtgtaatatc tgcctgctcc agcattttat ccatcgctcc gaaagcagcc ctgctcttcg
     3361 caccagccac agagaattgc tcctcatcat gagaggcatc ttttatagga ggataaactg
     3421 catatagaat accatacaat gtaaaaatta atgcctctgt agtaagacca atccacaacc
     3481 acgtatctgc atcgggttgg tgggtaattt ttctgagtgc accccagatt actaatgcgg
     3541 cgccaaatga tacgcctacg tcaacccatt tgcttacttt agatggaata gctgctgcca
     3601 taattggatt ttttaaagtg gttttgttga tattaattag gagagaaaaa agagaggaca
     3661 ggttctaatt ttactgccct ctcagtattt taattgtagt tcgacatttt atttgcgacg
     3721 tcctttttgc ggtgctgcgg gtaagtcaat cacacaacgg aagccgatat aagatttggt
     3781 agaatcctgg tattcataag tgctggtacc agtttgcagg taatatccaa catctttcca
     3841 gctaccacca cgaataatct tacgcttcat caggggagga tcatcatctt tcgcattcca
     3901 acgaacatcg gggttcatat catgctgaaa attatttcga ccctcgtaat agatagaaga
     3961 agtccactct gctacattgc cagacataca atacagacca taatcatttg gccagtaggc
     4021 atcggcacgg acagtataga atccgccatc ttctggatag ttaccacgac caggtttgaa
     4081 gttggccatc aaacaacctt ttttattacg caggtaatag ttaccccagg gaaacattga
     4141 ttgagaacga ccgccacggg ctgcatattc ccactgcgct tctgtgggca aacggaaatc
     4201 agactcctgg gtccttttct tagaatcaag ataggcattc aggaaatggg ttctccactc
     4261 acagaaagca gttgcttgtt tccagtttac accaactacc ggatagtttc caaaagccgg
     4321 gtgagaaaaa tatcttttgg tcatcggctc attgtatgaa taagagaagt cgcggatcca
     4381 ggctaaagta tccggataga tacccacatc cttttttaca ataaatgtag aacgaggctt
     4441 gcctgcattt tcccttttgg cagcttcctt cagatcgaag gtttcagaat gaaatacaat
     4501 ttttgatgga tcaatttctt ttttaccgaa aacccggtca tccggaggca aaataatatc
     4561 tgtactgctg agctgctcca ttactttagg atcgccccat ttcatagttt tcattttagc
     4621 ccagtcaaca tattcattac catcctggcc ggcttttaca taacccattt tacgggctac
     4681 gacagaatca cggacccagt acacaaactg acgatactca ttattggtaa tttcagttgc
     4741 atccatccaa aagccactga ttgatacaga acggtttcgg gcactgaatg cataagccgg
     4801 atcctcatca ctgggaccca tatggaatgt accctgtggc acatacacca tgcccggagg
     4861 cttcggaagt gaatacttac ttccaggagc aactccatgc acctgaccat cattaggaag
     4921 gctaccgcct ttgtttttct ttccacccag tatgccgcaa cttgcaaaca ttgtcatgca
     4981 ggaagctgct aaaagggtgt aataaaggtt tctcattttc atacacttaa agggtattgg
     5041 gcaaatataa tatttagaac tattctctgg cacaaggtta taatttattt ttttaaatca
     5101 aaacgtattt ttcgaggtaa attttctaac ggtgaccggt ttcattttat tgtattaact
     5161 taaaattaat tattgctttt ggcccttgtc tatcagcgat ataagctcct ttgcagaaaa
     5221 tacgggatta aggcaagtgt aactacggca caggtatatc gtaggtttat cagcagtcat
     5281 tttgccagct aataatggga agctattatc ccccttttca gcggtcatta aaacccggtg
     5341 aggaatatat tgttgaagga tttctgtatg caggttatac aaatttttac ctactaccgc
     5401 tatctcactg gtcccggcaa ttatttcctg caacaggcat gcccagttac caaaggaagt
     5461 tggataccgg ataatggcag tattgaggga taagatcata tccagacttc tcttgcccca
     5521 ttccctttta ccgaacaaaa cagatagatg gtaaagatta taggccatta ctgaattacc
     5581 tgatgggaca gcgccatcat aaacctcttt tttcctgata ataacatcct gctgaccagc
     5641 aggagtgtaa aaaaagaaag gagtttcctc atcactaaaa ttctcaat
//
LOCUS       DAORTI010000180         5653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17163_length_5653_cov_4.377992, whole genome shotgun sequence.
ACCESSION   DAORTI010000180 DAORTI010000000
VERSION     DAORTI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_5653_cov_4.377992"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLO70_15285"
     CDS             complement(<1..548)
                     /locus_tag="PLO70_15285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ75883.1"
                     /translation="MAKIGLVTVLFKSDEVLPDFFSSIARQTHKDYILYLVDNSESEA
                     TDKMISQCLADYPVTAYKHIRTGGNIGVAAGNNTGIKLSIADHCSHVLILNNDIDIEQ
                     DFMFSAMLSLAEEKGESMIVPKIYYYGEKKIWMAGGYMDKWRALGVHYGYNKADAPEY
                     NVAKHVTYSPTCFMLVKREVFE"
     gene            complement(555..1697)
                     /locus_tag="PLO70_15290"
     CDS             complement(555..1697)
                     /locus_tag="PLO70_15290"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ75884.1"
                     /translation="MGYKKVLITGGAGFIGSNLGLYLVDKGYEVTVLDNLSEQIHGAD
                     PAATSPLYISIKDKVNFIKGTVTARTDWEKAIGDNEVIVHLAAETGTGQSMYQVSKYA
                     EVNMGGTAIMLDILANRKTNVRKVIIASSRAIYGEGKYISEELGVVYPAHRLASDMDA
                     GNFEVTYPGATKPLKLVATDEDSKIHPSSVYGITKQNQEQMVMTVCPTIGIAPVAFRY
                     QNVYGPGQSLSNPYTGILSIFSTLIRNKKAINIFEDGKESRDFVYIDDVVRATFLGIE
                     MEAANGQVFNVGTGVATTVLEVATLLADYYNEQVPITVSGNYRLGDIRHNLADMTKIE
                     KLLSFRPGVTFKEGLKKFTEWVSLQQIQRSKYEESITEMKAKGLLK"
     gene            complement(1709..2302)
                     /locus_tag="PLO70_15295"
     CDS             complement(1709..2302)
                     /locus_tag="PLO70_15295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQZ75885.1"
                     /translation="MLRKIGLLFYYLFKYPVYKILFAKIGSRSRLLGTRVEGYNRIQI
                     GSKVYINTGGWLACIPLTGNADCLLSIGDGTYIGRYSHIYATSKIEIGKKVLMADKVY
                     LSDNLHGYKNINLPVIDQPVEQLHPVIIKDGAWLGENVCVVGATVGKNSVVGANSVVT
                     KDIPDHCVAVGSPAIIIKRYDFKKNEWCKTDKEGNFI"
     gene            2402..3352
                     /locus_tag="PLO70_15300"
     CDS             2402..3352
                     /locus_tag="PLO70_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75886.1"
                     /translation="MAKLRRGLQQSCKLATFDLIRNDSMNIFQRLTIAIFKEEAKRKI
                     FTKALSKLSPPNLYPVTDPGAVHFKHFGLIGDIIYAIPAMKALAGEKKIHLHLQINQK
                     SLYRKSMKHANKGKILTEKSVEMLAPLILAQPGFAACDILTDQKIDYDLDEFRKYPFD
                     YNTNHICRWYFHMYGITADLGKPWLTVTPDTSFNNEIVIARSFRYRAPGISYDFLQAY
                     PNISFVGLKEEYEDLKKAIPALKYKNVSNFLEFAGIIAGCKFFIGNQSFPFAVAEGLK
                     VKRVLEVCFECPNVIPEGKDAYDFCYQPQFEKIVKQLYNH"
     gene            3411..4139
                     /locus_tag="PLO70_15305"
     CDS             3411..4139
                     /locus_tag="PLO70_15305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ75887.1"
                     /translation="MNEQINDTVKKSAYFSNRNVDEKSYIDYILPRYLNPYFTETDKE
                     KNILDIGCGLGQMLTYLKGKGFNSLYGIDINDESINLCKKHGLDVEKVNDIREFARNS
                     NRKFDRIVMSHVLEHISKEDIIDTLIHIKKYLLKEGGIFLLMVPNAQSYTGAYWRYED
                     FTHNIMFTAGSCTYVLRSAGFENIEFLDPDGTMYMNPVKRLIIKFLLGYYKLKENAWN
                     KILQTSYHKPSPRIYTFELKVAAS"
     gene            complement(4160..4909)
                     /locus_tag="PLO70_15310"
     CDS             complement(4160..4909)
                     /locus_tag="PLO70_15310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HQZ75888.1"
                     /translation="MKKLLINILSKTGYRIIRKSKLNFSVGDFQYEQITPFADYAPWL
                     QDKEFTQIYSRIKDYTLVDIYRCYELWEMVEAIHVINPTVCFLEVGVWKGGTSGIIGR
                     KLALLNSTNNFYLADTFSGVVKATEKDTFYKGGEHADTAMETAEGIIKSVYKNYKILK
                     GIFPNDTATIINKDERFGFCHIDVDVYQSAKDIVDWIWDKLIIGGVIVFDDYGFHTCT
                     GVTRYVNEQKGKSDRAVIHNLNGHAIIFKLR"
     gene            5178..>5653
                     /locus_tag="PLO70_15315"
     CDS             5178..>5653
                     /locus_tag="PLO70_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQZ75889.1"
                     /translation="MGIKNPTYLDIGANHPIALSNTYRLYTLGCRGVLIEPNPFLYKK
                     LLQKRKGDKCINIGIAFDENTEADFYIFPEKYHGLNTFSKKEADFWTNTGNEEIGKQN
                     IEKIIRIPLVNINEIMGEYFTPYPNFVSIDVEGLDLQIVKTIDFENFWRKSGFANKR"
BASE COUNT         1784 a         1205 c          959 g         1705 t
ORIGIN      
        1 ttttcaaaca cttccctttt taccagcatg aaacaggtag gtgaataggt tacatgtttt
       61 gctacattat attccggggc atcagcttta ttataaccat aatgcactcc caaagccctc
      121 cacttatcca tatatcctcc cgccatccag atttttttct ccccgtaata ataaatcttc
      181 ggaacgatca tgctctcccc tttctcttca gcaagggaaa gcatagcact gaacataaag
      241 tcctgttcta tgtcaatatc attattcaat atcagtacat gagaacaatg atcagcaatg
      301 gataatttta ttcctgtatt attcccggct gccactccaa tattaccacc ggtcctgata
      361 tgtttgtacg ccgtaaccgg atagtccgcc aggcattggc tgatcatctt atccgttgcc
      421 tcactttcag aattatccac taaataaagg atataatctt tatgagtttg ccgcgcaata
      481 ctgctgaaga aatccgggag cacttcatca ctcttaaaca aaacggtaac taatccgatc
      541 ttggccatac tttattattt caataacccc tttgctttca tttcagtaat agattcttca
      601 tatttgcttc tttgaatttg ctgcaggctt acccattcag taaatttttt aaggccctct
      661 ttgaacgtaa cgccgggcct gaaagaaagc agtttttcaa tcttcgtcat atccgccagg
      721 ttatgacgga tatcacccaa cctgtaatta cccgaaacag ttatcgggac ctgctcatta
      781 taataatcag ccagtaatgt ggctacttcc agtacggtgg tagccacgcc ggttccaaca
      841 ttaaaaacct gtccgttggc agcttccatt tctattccca ggaaggttgc tcttaccaca
      901 tcatcgatat aaacaaaatc ccggctctct tttccatcct caaaaatgtt gatcgctttt
      961 ttattcctga tcagggtaga aaaaatagaa agtatacccg tgtaaggatt acttaatgat
     1021 tgtccgggcc catatacatt ctggtaacgg aaagcaacag gggcaatacc aatggtgggg
     1081 caaacagtca tcaccatttg ttcctggttc tgtttggtaa tgccatacac cgatgaagga
     1141 tggatctttg aatcttcatc cgttgccacc agtttcaaag gctttgtcgc cccgggataa
     1201 gtaacttcaa aattcccggc atccatatca ctggccaggc ggtgagccgg gtatactaca
     1261 cccagttctt ccgagatata cttcccttcc ccataaatag cccttgaaga agcaataata
     1321 actttcctga cattggtttt acgattggcc agtatatcca gcatgatagc ggtgcccccc
     1381 atattaactt ctgcatattt actgacctgg tacatcgatt gaccagtgcc tgtttcagca
     1441 gccaggtgta caatcacttc attatctcct atcgctttct cccaatctgt tcttgcggta
     1501 acagttccct ttataaaatt tactttatcc tttattgaaa tatacaaggg cgaagtggca
     1561 gcaggatctg caccatgtat ctgctcagat aaattatcca gcaccgttac ttcatagcct
     1621 ttatcaacca ggtaaagtcc caggttggaa ccaataaatc ctgcaccgcc ggtaattaaa
     1681 actttcttat atcccattac tattgatttc agataaagtt tccttcttta tcagttttgc
     1741 accattcatt tttcttaaaa tcataccgct tgataatgat agcgggagaa cccactgcca
     1801 cgcaatgatc aggaatgtct tttgtcacca cactattggc tcccaccaca ctattctttc
     1861 caactgtggc gcctaccaca caaacatttt cacccaacca ggccccgtct tttattatta
     1921 caggatgtaa ttgttccacc ggctggtcaa taacaggaag attaatattc ttgtaaccat
     1981 gcaaattatc actcaggtag accttatcag ccatcagtac ttttttaccg atctcaattt
     2041 tagaagtggc atagatatga gagtaccggc caatataggt tccatcgcca atggaaagaa
     2101 ggcaatcagc atttcctgtc agtgggatgc aggccagcca accaccggta ttgatatata
     2161 ctttactgcc gatctgaatc cggttgtatc cctctaccct ggtacccagc agccgggaac
     2221 gggaaccgat ctttgcaaac aggatcttgt aaacaggata cttgaataaa tagtagaaca
     2281 ataaaccaat tttcctgagc ataccggccg ttatttcatt acttaaatta ttacaaatac
     2341 ccgttctctt tactgccaaa acagacgatg gcaaggtaat acatttccgg taatagaata
     2401 aatggcgaag ctccggagag ggcttcaaca aagctgtaaa ttggctactt ttgacctcat
     2461 cagaaatgac agtatgaata tttttcagcg gcttactatt gcgattttta aagaagaggc
     2521 gaaaagaaag atctttacca aagccctttc aaaactaagt cctcccaacc tctatcctgt
     2581 aactgaccct ggtgccgttc atttcaaaca tttcggcctg atcggtgata ttatttatgc
     2641 catacccgcc atgaaggcac tggccggcga aaagaaaatt caccttcacc tccagatcaa
     2701 tcaaaaatcg ctgtaccgga agagtatgaa gcatgctaac aaggggaaga tcctgacaga
     2761 gaaaagtgtg gagatgctgg caccccttat ccttgcgcag ccgggatttg ccgcctgcga
     2821 tatattgacc gaccaaaaga tcgattacga cctggatgaa ttcagaaaat acccttttga
     2881 ttataatact aaccatatct gccgctggta tttccatatg tacggcatta cagctgatct
     2941 tggtaaaccc tggctgaccg taactccaga tacttcgttc aacaatgaga ttgtgatagc
     3001 acgcagtttc cggtaccggg cacctggtat cagctatgat tttttacaag cctatcccaa
     3061 tatcagcttt gtgggactta aagaagaata cgaggatttg aaaaaagcga tacccgcatt
     3121 gaaatataaa aatgtttcca atttcctcga attcgccggg ataattgccg ggtgtaaatt
     3181 ctttatcggc aaccagtcat ttccttttgc cgtagcagaa ggattaaaag taaaaagagt
     3241 gctggaggtt tgttttgaat gtcccaatgt cattccggaa gggaaagatg cctatgattt
     3301 ctgttaccag ccgcagtttg agaaaattgt gaagcagttg tataaccatt aacctatgag
     3361 aatacaagat acaagccata tcttcaatcc taatttttat accaaaaaca atgaatgaac
     3421 agataaatga tacggtaaag aaaagtgcat atttctcgaa tcggaatgtt gatgagaaat
     3481 cttacatcga ttacatcctt ccgagatacc tgaatccata ttttacagaa actgacaaag
     3541 aaaaaaatat tttagatatt ggttgcggac ttggccaaat gcttacatat ttaaaaggca
     3601 aaggattcaa tagcctgtat gggatagata taaatgacga atcgataaat ttatgtaaaa
     3661 agcatgggct ggatgttgaa aaagtgaatg acataagaga gtttgccaga aatagtaaca
     3721 gaaagtttga caggattgtt atgagtcatg ttttggaaca tattagcaag gaagatatta
     3781 ttgatacgct aattcatatt aaaaaatatc ttctcaaaga aggaggaata tttctgttga
     3841 tggttcccaa tgctcaatct tatactggag cttattggcg ttatgaggat tttacacata
     3901 acataatgtt cactgccgga tcatgtactt atgtcctcag atctgcaggg tttgaaaata
     3961 tagaattcct tgatcctgac ggcaccatgt atatgaaccc tgtaaaaaga ttaattataa
     4021 aatttctact cggttattat aaactaaaag aaaatgcctg gaacaagata ttacaaacgt
     4081 cataccacaa acccagccct aggatctata ctttcgaatt aaaagtagct gcaagctaat
     4141 aattgaagaa tgtgacaacc tatcttagtt tgaatataat agcatgcccg tttaaattat
     4201 gtattacagc cctgtctgac tttccttttt gttcgttaac atacctggta actccggtac
     4261 atgtatgaaa cccgtaatca tcaaaaacta ttaccccacc tataatcaat ttatcccata
     4321 tccaatcaac aatatctttt gccgattgat aaacatcaac atctatatga caaaaaccaa
     4381 acctctcatc cttattaatt attgttgccg tatcatttgg aaaaatgcct tttaatatct
     4441 tgtaattctt gtatacactc tttattatcc cttctgctgt ttccattgct gtatcagcat
     4501 gctctccccc tttataaaaa gtatcttttt ccgtcgcctt tactacaccc gaaaaagtat
     4561 cagccagata aaaattgtta gttgagttca ataatgctaa ctttctaccg ataattccag
     4621 aagtaccacc tttccataca ccaacttcaa gaaaacaaac cgtgggatta ataacatgaa
     4681 tagcttctac catttcccat aattcataac accggtaaat atcaactagt gtatagtctt
     4741 ttattcggct atatatttgc gtaaattcct tatcctgtag ccagggagca taatctgcaa
     4801 atggtgttat ttgttcatat tgaaagtctc caacagaaaa attcaacttt gactttctga
     4861 taatcctgta accagttttt gacaaaatat ttatcaataa ttttttcata cttacatata
     4921 gatttaatcc cagcaagtaa aaaaacaact ttactaccat agttacaaat atttttcact
     4981 ttgtaattcc catttataga aatgaaccct acagaacaaa aactagttta tgtatcttag
     5041 ctcgttgtaa atcaaaaaaa atgacttcta tcaagactgg cttaagaaaa atattagtta
     5101 attccttttt tgcccatatg cgaagaagtt attctcaaag cggggaagac atcatagtta
     5161 gctacttatt tgaaagaatg gggataaaaa accctaccta cctggatatc ggagctaacc
     5221 atccaatagc gttgagtaac acgtatcgtc tttatacatt agggtgccgg ggtgtactta
     5281 ttgaaccaaa cccatttcta tataaaaaac ttttacaaaa aagaaaaggt gacaaatgta
     5341 tcaatattgg tattgctttt gatgaaaata ctgaagctga tttttacatt ttcccggaaa
     5401 agtaccatgg gctaaatacc ttctcaaaaa aagaagctga tttttggaca aataccggaa
     5461 acgaagaaat aggcaaacaa aatatcgaaa aaataatcag aatccctctt gttaatatta
     5521 atgagataat gggagaatac tttactccat atcccaattt tgtttcaata gatgtagaag
     5581 gattggatct tcaaattgta aaaacaattg attttgaaaa tttttggagg aaatcaggat
     5641 ttgcaaataa acg
//
LOCUS       DAORTI010000181         5643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17206_length_5643_cov_7.017180, whole genome shotgun sequence.
ACCESSION   DAORTI010000181 DAORTI010000000
VERSION     DAORTI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17206_length_5643_cov_7.017180"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..892
                     /locus_tag="PLO70_15320"
     CDS             137..892
                     /locus_tag="PLO70_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75890.1"
                     /translation="MEVHHHAHTADPGSHRGKKKWTHYFWEFLMLFLAVFCGFLAEYQ
                     LEHKIERDREKKFIQTFIEDLKTDTAAIRQNIIFRKNKMKAMDSLTFLLNSQQIKGYE
                     NDLYYLGRSFVRGVRFQSNDRTITQLKNSGSLRLIRNEKAADSMISYQKLVEYIYYNQ
                     EDDRIERREADPLLSHIFYPAVFDKMVTIDGITRPTDNPPLRSYDKNLHFDLAYYVHQ
                     LKGSNFMIEVRLKLLNEKAINMITFLQKEYHLK"
     gene            900..1640
                     /locus_tag="PLO70_15325"
     CDS             900..1640
                     /locus_tag="PLO70_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75891.1"
                     /translation="MEVHAHSHTPRNKWTHYFWEFLMLFLAVFCGFLAEYQLEHKIEK
                     DREKQFIKSLVADLQDDEKILSTMTTSEQFGIASLDTLMDLLNNPAMAKQNGDQLYYV
                     ARMGPRYIPFANNSRTFDQLKFSGGFRLIRSLDASNKIMDYYNQFSLLRLLESNYNQE
                     FDNYKIIAAKILDPAILRRQETEKGVILRSPDNPALRTYDTELLKELGFHTLQMNGSR
                     RSKLFILESLKKGAEELMLYLINKYHLE"
     gene            1668..2876
                     /locus_tag="PLO70_15330"
     CDS             1668..2876
                     /locus_tag="PLO70_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQZ75892.1"
                     /translation="MQPLTAPHQKRIESIDIIRGIAMVLMALDHTRDYFHIGANVDDP
                     LNLATTSPALFFTRWITHFCAPIFVFLSGTSIYLQSLRKTKKELGIFLLKRGLWLIIV
                     EWTIITFAWTFNPFYNFIAFQVIWAIGISMVILGLMVLIRLPYKVIFILGVIIVAGHN
                     LLDIPEAAPGFQASFAWDLLHHGVFRPYEFSPNRFAFLVYPFPAWTGVMMLGYCAGAL
                     FTTEYTSEQRRKIITRIGLGLIAFFIALRFTNVYGDPVDWTTQKNGFYTFLSFINVDK
                     YPPSLLYLCITIGPALLLLAFMERIKNNFTNVMVVFGRTAFFYYIIHIYLIHLLAAIC
                     FFARGHTLEDAKNVGEKFPFLFVAPGEGYNLWVVYGVWLAVVISLYPLCKRYDQYKTN
                     HKEKWWLSYL"
     gene            2908..4404
                     /locus_tag="PLO70_15335"
     CDS             2908..4404
                     /locus_tag="PLO70_15335"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQZ75893.1"
                     /translation="MLYIFLKARFLILAATVCICLNATAQSTIEYDIVLSGGRVIDPE
                     TKLDAIRNVGINNNLIAQISTETLKGKQTINVSGLVVAPGFIDMHIHGRSNVEQEYQL
                     HDGVTTALELEWGIEHLGKWYASRKGKALINYGASVNWPFERFKAIGKYQNAVDSLLQ
                     ITLRGEANIGAMTNTILRAATETITPDALNQTLANIKASLAEGGIGIGAPIGYLPKTN
                     PNEMFQVYKLAGELQALVFSHVRQPDIISIQEAIADAVLTGAPLHIVHINSMSLGNIG
                     LSLEMVNAANKKGFDISTELYPYTAGSTSLQSAMFDEGWQQRLGISYGDLQWVATGER
                     LTKETFDAYRKTGGVVILHVMKPEWIQTGIAAPGVMIGSDGMTYAKLAHPRTAGTFSR
                     VLGKYVREEKVLDLNTAIEKMTLLPAKRLENIAPSMRFKGRIQVGADADITIFNPETI
                     IDKATFEKGLEFSAGIEYVLVNGSFVLKNGKTVSNVFPGQAVYGKFKK"
     gene            4408..4851
                     /locus_tag="PLO70_15340"
     CDS             4408..4851
                     /locus_tag="PLO70_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ75894.1"
                     /translation="MKKIIILLIATVLTRQLYAQGQLTRNQQAVQQAVLKLFNALSNR
                     DSVNLKLFCADDITLYEYGQIWNLDTLINKAIRLNTASDFKRINTIDFINTTISKKAA
                     WTTYLNQADINRDGKQTTVKWIETVVLVKQQKRWKIKVLHSTLIK"
     gene            4878..>5643
                     /locus_tag="PLO70_15345"
     CDS             4878..>5643
                     /locus_tag="PLO70_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQZ75895.1"
                     /translation="MKKKSIFLLAILFAGLLQAQDNSGLNLSINHIALSVKDVDRSAA
                     FYKTVLLLPEITNRTKIEGIRWVTLADDRELHLISILKEPVTINKAVHLALTTDNFDA
                     VLKRLADLKITYSDWPGIPNTFTSRADGVKQLYFQDPDGYWIEVNSVNDNLVSVEQIK
                     NEIWQLEENYWKYVKEKDYQSYITLWDDRFIGYPSNNTIGNKDHITDWMTEMYRQPGV
                     FNYTLTRKVENVFGDIAIVFYDVSHHFTNDKNEIVKK"
BASE COUNT         1839 a         1039 c         1151 g         1614 t
ORIGIN      
        1 atttcgcctc aattacagaa tacatatgtt gtctatttat aacaagacct tgcgatacca
       61 ggcgcggagc ctgaaagaaa atgccgttaa actgataatc cagttgaaaa aagaatacaa
      121 gttgaaataa taaattatgg aagtacacca ccatgctcat accgccgacc ccggtagcca
      181 ccggggcaag aaaaaatgga cgcattattt ctgggagttt ttaatgttgt tcttggctgt
      241 gttctgcgga tttttagcag agtaccagct ggagcataaa atagaaagag acagagagaa
      301 aaagtttata caaaccttta ttgaagattt aaaaacagat acggcagcca tcaggcaaaa
      361 tatcattttc aggaaaaaca agatgaaagc aatggattct ttaacgtttc tcttaaacag
      421 tcagcaaatt aaagggtatg aaaatgatct ttattattta ggcagaagtt ttgtgagggg
      481 tgttaggttt caaagtaatg atcgaaccat tacacaatta aaaaattccg gctccttaag
      541 attgatacga aatgaaaagg ccgccgacag tatgatatca tatcaaaaac tggtagaata
      601 tatttattat aaccaggaag acgatcgtat cgaaagaaga gaggcagacc ctctattatc
      661 ccatatattt taccctgcag tatttgataa aatggtaact attgatggca tcaccaggcc
      721 aactgataat ccacctttaa gatcatacga taaaaacctt cactttgacc ttgcttatta
      781 tgttcaccag cttaaaggca gcaatttcat gattgaagta aggttgaaat tgttgaatga
      841 aaaagcaata aacatgataa catttttaca gaaagagtat catctgaaat aaaatattta
      901 tggaagtaca tgcacactca cataccccaa gaaataaatg gacccattat ttctgggaat
      961 ttttaatgtt gttcctcgct gtgttctgcg ggttcctggc tgagtatcaa ctcgaacata
     1021 agattgaaaa agaccgggag aaacagttta taaaatcact ggtggccgat ttgcaggatg
     1081 atgaaaagat cctgtcaact atgacaacct cagaacaatt tggaatagca agtcttgata
     1141 cattaatgga tttactgaat aatccagcca tggcaaaaca aaatggggat cagttgtact
     1201 atgtggcaag gatggggccg agatatattc cctttgccaa caacagcaga acctttgatc
     1261 agctgaaatt ttccggaggt ttccgtctga tccgctcact cgatgcctcc aacaaaataa
     1321 tggattatta taaccagttt tcccttttgc ggttactgga aagtaattat aaccaggagt
     1381 ttgataatta taaaatcatt gcagcaaaaa ttcttgaccc ggctatttta agaaggcagg
     1441 aaactgaaaa aggagtaatt cttaggagtc cagataatcc tgctctcaga acatatgata
     1501 cagaactcct gaaagagctg ggatttcata ctttacaaat gaacggttca agacgatcaa
     1561 agttattcat attggagtca ttgaaaaaag gagcggaaga gttgatgctt tatttaataa
     1621 ataaatatca cctggaataa agaaatagta tccatcaaaa ccaccacatg caaccactaa
     1681 ctgctcctca ccaaaaaaga atcgaatcca ttgacatcat ccgtggaatt gccatggtac
     1741 taatggcact cgatcatacc agggattatt ttcatattgg agccaacgtt gatgacccgt
     1801 tgaacctggc cactacctca ccagcattat tctttacccg gtggatcact catttctgtg
     1861 cccccatctt tgtttttctg tcgggtactt ctatttactt acaaagcttg cgcaaaacaa
     1921 aaaaagaact cggcatcttt ttactcaagc ggggcttgtg gctgataatt gttgagtgga
     1981 ccattatcac ttttgcatgg accttcaatc ctttctacaa tttcattgct ttccaggtta
     2041 tctgggccat tggtatcagc atggtaatac tggggttaat ggtgcttatc aggcttcctt
     2101 ataaagttat atttatactc ggtgttatta ttgtcgctgg tcataattta ttagacattc
     2161 cggaagcggc tcccggtttt caagccagct ttgcctggga tctgttgcat cacggtgtat
     2221 ttagacctta tgaattttca cccaatcgtt ttgcctttct ggtgtatcct tttcccgcat
     2281 ggacgggtgt gatgatgctt ggctattgtg ccggggcatt atttactacg gaatatactt
     2341 cagagcaacg aagaaagatt ataacccgta tcggactagg attgattgca ttttttatag
     2401 cccttcgttt caccaatgta tatggtgatc cggtggactg gaccacacag aaaaatggtt
     2461 tctatacttt tctatctttt ataaatgtag ataagtatcc cccttcttta ttatatctct
     2521 gcatcaccat cggcccggcg ttattgctct tagcgtttat ggagagaatt aaaaacaatt
     2581 ttaccaatgt gatggtggta tttggcagaa ctgctttctt ttattatatc attcatatat
     2641 atctgattca cttgctggca gccatttgtt tttttgcccg tggccatacc ctggaagatg
     2701 caaaaaatgt tggagagaaa ttcccttttt tattcgtagc acccggcgaa gggtataact
     2761 tatgggtagt gtatggtgtt tggctggcag tggttatttc gttgtatccc ttatgtaaac
     2821 ggtatgatca atataaaacc aatcataaag aaaaatggtg gctgagttat ttataaaaaa
     2881 actttcacac tttaaaactt aggaaaaatg ctatatattt ttttaaaagc aaggttcttg
     2941 attcttgcgg cgacagtttg tatttgctta aacgcaacag cacaatcaac cattgaatat
     3001 gacattgtac tatccggtgg tcgggtaatt gatcccgaaa caaaattgga tgctatacga
     3061 aatgttggca tcaacaacaa tctcattgca caaatttcaa ccgagacatt aaaaggaaaa
     3121 caaactatta atgtaagcgg attggtggtt gctcccggtt ttattgatat gcacatacac
     3181 ggccgcagta atgtggagca ggagtatcaa ttgcacgatg gcgtaacaac tgcgttagag
     3241 ttagaatggg gaattgaaca tttagggaaa tggtatgcat ccagaaaagg aaaagccctg
     3301 attaattacg gtgccagtgt aaactggcct tttgaacggt ttaaagcaat tggcaaatac
     3361 cagaacgctg tagatagttt actgcaaatt acgctgaggg gagaagcaaa cattggggcc
     3421 atgaccaaca ctattttacg ggccgctact gaaactatta ctccagatgc attgaatcaa
     3481 acattggcaa acatcaaagc ttcattggct gaaggtggta ttggcattgg tgcacccatt
     3541 ggttatttgc ccaaaacaaa tcccaatgaa atgttccagg tgtataaact ggctggtgag
     3601 ttgcaagccc ttgtgttttc gcatgtaagg caaccggata ttatttctat ccaggaagca
     3661 atagccgatg cggtactcac cggcgcaccg ctgcacattg ttcatatcaa cagtatgtcg
     3721 ctgggaaata ttggactgtc gctggaaatg gtcaatgctg caaacaaaaa aggttttgac
     3781 atcagcactg agttgtatcc ttataccgct ggttccactt cgctgcaaag cgccatgttt
     3841 gatgaaggtt ggcaacaaag attaggaata agctacggcg atctgcaatg ggtggcaaca
     3901 ggcgaacggc ttacgaaaga aacatttgat gcgtaccgta aaacaggtgg cgttgtgatt
     3961 cttcatgtaa tgaaacccga atggatccaa acgggtattg ctgctcccgg tgtaatgatt
     4021 ggttcggatg gaatgactta tgcgaagctt gctcatccaa gaaccgctgg aacattttca
     4081 agagtgttag gtaaatatgt acgggaagaa aaagtgcttg acttaaatac cgccatagaa
     4141 aaaatgacct tactacctgc aaaaagattg gaaaacattg ctccctctat gcgttttaaa
     4201 ggaaggatac aagtgggtgc tgatgctgac attaccattt ttaatcctga aactattatt
     4261 gacaaagcaa catttgagaa aggcctcgag ttttctgcag gtattgaata tgtactagtg
     4321 aatggaagtt ttgtgttgaa gaatggaaaa acagtaagca atgtttttcc ggggcaggcg
     4381 gtgtatggga agtttaaaaa gtaattcatg aaaaaaataa taattctttt gattgcaact
     4441 gtactgacca ggcaattata tgcacagggc caattaacaa gaaatcagca ggccgtacag
     4501 caagcagtcc tcaaactatt taatgcttta tccaaccggg attcagtaaa tttaaaacta
     4561 ttctgcgctg atgatataac attatatgaa tatggccaga tctggaatct ggatacactg
     4621 atcaacaagg caatcaggtt aaatacagct tcagatttta aacggatcaa taccattgat
     4681 tttataaaca caactataag taagaaagca gcctggacca cttatcttaa ccaggcagat
     4741 ataaacaggg atggaaaaca aacaacggtg aaatggatag aaactgttgt tttagttaaa
     4801 caacaaaaaa gatggaaaat taaagtattg cattcgacac tcataaaata aatactaaaa
     4861 gatcacaaag aataaatatg aaaaaaaaat caatcttcct tcttgccatt ttatttgcag
     4921 gattattgca ggcacaggat aattcgggct tgaatctttc catcaaccat attgcccttt
     4981 cggtaaaaga tgtggatcgc tccgcggcat tttacaaaac agtactattg ctgccggaaa
     5041 taaccaaccg taccaaaata gaaggtatcc gctgggtaac attggccgat gaccgggaac
     5101 tgcacctgat ctctatttta aaagaaccgg taaccattaa taaagcagtg cacctggcgc
     5161 ttaccaccga taattttgat gctgtattaa aaaggttagc tgatttgaaa attacctatt
     5221 ccgactggcc gggaatacca aatacgttta ccagcagggc cgatggggta aagcagttat
     5281 acttccaaga tcccgatgga tattggatag aggtaaacag tgttaatgac aacctggttt
     5341 ctgtagaaca aatcaaaaat gaaatttggc agttggaaga aaattactgg aagtatgtaa
     5401 aagaaaaaga ctatcaatca tatatcacat tatgggatga tcgtttcatt ggctacccaa
     5461 gtaataatac aatcggcaac aaagatcata ttacagattg gatgacggaa atgtaccggc
     5521 aacctggtgt ttttaattat acacttaccc gtaaagtaga aaatgtattt ggcgatattg
     5581 caattgtatt ctatgatgta tcgcaccact ttaccaacga taagaacgaa atagttaaaa
     5641 aag
//
LOCUS       DAORTI010000182         5602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17373_length_5602_cov_7.376780, whole genome shotgun sequence.
ACCESSION   DAORTI010000182 DAORTI010000000
VERSION     DAORTI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17373_length_5602_cov_7.376780"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1627)
                     /gene="gpmI"
                     /locus_tag="PLO70_15350"
     CDS             complement(101..1627)
                     /gene="gpmI"
                     /locus_tag="PLO70_15350"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQZ75896.1"
                     /translation="MSAKKVILIIMDGWGLGKVASSDAIQHANVPFTKSLYSKYPNTT
                     LVTCGEAVGLPDGQMGNSEVGHLNLGAGRIVYQELQRINVAVRDGSFAQNQKLLSAVR
                     FAKENNKPLHLLGLVSNGGVHSHINHLKAIVDVCKAEGLKDVFIHAFTDGRDCDPKSG
                     LAFMIELQQHLNYSVGKIASVSGRYYAMDRDKRWERVKLAYDTLINGEGPKATDAIAA
                     VENSYAQNITDEFIKPTVIVNEAQQPLATIKNGDVAICFNFRTDRCREVTQVLTQLDM
                     PEFGMNKLSLHYTTMTEYDKTYNNVQVIFETDNLNNTLGEILEKNGLKQIRIAETEKY
                     PHVSFFFSGGRENPFEGEKRIMVPSPKVATYDLQPEMSALEVTDAIVSEIEQKTADFI
                     CLNFANADMVGHTGIWEAAIKAVETVDKCVERVVTTGLTNGYSIFLTADHGNSDFMIN
                     EDGSPNTAHTLNPVPFFIIDKDWKGPIKPGKLGDIAPTILTLMQLPIPKDMTGNVLID
                     "
     gene            1833..2222
                     /locus_tag="PLO70_15355"
     CDS             1833..2222
                     /locus_tag="PLO70_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HQZ75897.1"
                     /translation="MKQIFTTLLLSAVLMLSSFKAQDGIDEVIGALKSGNATELAKYF
                     DDTVELTLPDKSDSYSKAQAQLIVKDFFTNNGVKGFELKHKGTSTGGYFAIGTLQTNS
                     GNFRTNIFMKTKSGKDVVKEIRFQSID"
     gene            2321..3178
                     /gene="nadC"
                     /locus_tag="PLO70_15360"
     CDS             2321..3178
                     /gene="nadC"
                     /locus_tag="PLO70_15360"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQZ75898.1"
                     /translation="MNFDEQLHHLIDEALKEDVGDGDHSTLSCIPAGAKGKAVLKIKQ
                     GGVLAGMQVALKIFSYKEPASVFTSFKEDGEEMKYGEKAFEVEASVHTILQCERLVLN
                     CMQRMSGIATLTKQYTGKLKGYKTRLLDTRKTTPNFRLLEKEAVRIGGGVNHRFGLYD
                     MIMLKDNHIDYCGGIENAIEQAFDYVQTKKPDLKIEVETRNLDDVRKVIAIGKGKVFR
                     IMLDNFTPEQIEEALGLIKGDFETEASGGINLDNIEDYAKIGVDYVSVGALIHQAKSL
                     DLSLKAVIV"
     gene            3181..3522
                     /locus_tag="PLO70_15365"
     CDS             3181..3522
                     /locus_tag="PLO70_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HQZ75899.1"
                     /translation="MSKKNKPDAMGFVYSTNPNFSFEEERKATETLPPAQQKLKVRLD
                     TKHRAGKAVTLIEGFTGKEEDAQELGKKLKSFCGTGGSVKDGEIIVQGDQREKVMQWL
                     LKNGYKNVKKT"
     gene            complement(3928..5214)
                     /locus_tag="PLO70_15370"
     CDS             complement(3928..5214)
                     /locus_tag="PLO70_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ75900.1"
                     /translation="MQTARYISMIANSNGYYEYLPTGYSTPGNQATYPLLIFLHGLGE
                     LGNGSAAQLPRVLFNGPPKIINQGNFPSSFTVNGQTFSFIVISPQFIDWPTPNDINNI
                     INYVSQNYRVNINRIYLTGLSMGGGATWEYAGDNSVYANRLAALLPVCGASWPESSRA
                     FTMAAANLPVWATHNNGDQTVPVSYTNDYITHYQNAPVTSSIAPKKTIFNVNGHDAWT
                     TTYNPSWRENGTLNVYEWLLTHQRTNIILPVELLEYSIVKSTSTSVFINWRTSQEQNN
                     NYFTVERSTNGQDFTSIAQVQGTNIFTGSSYNYEDIGLTSGTFFYRLSQTDLNGRRTY
                     YDTKKITIEDNESTVNLYPNPASEFITIEINTPATGNFIVTIFQNDGRQVKQIQFNKS
                     TDLFRTQITLAGIPSGYNTVEITGKDFKKNIAFIKL"
BASE COUNT         1695 a          999 c         1125 g         1783 t
ORIGIN      
        1 tgctttattt ttttttggat gtataaattg tattattacc agggctgcta ataataccag
       61 cataattttt ttaaacatac ttctcctttt agggttaaaa ttaatcaatc aggacattcc
      121 ctgtcatgtc ttttggtata ggcagttgca ttagagtaag aatcgttggt gcaatgtctc
      181 caagttttcc tggttttatt ggccccttcc aatctttgtc aattataaag aacgggaccg
      241 gattcagtgt atgggcagtg ttgggtgaac catcttcatt gatcataaag tcggaattgc
      301 catggtcagc agtaagaaaa atagaatacc cgtttgtaag cccggtagtt accactcttt
      361 caacacattt gtcaaccgtt tcaacggctt taatagccgc ttcccagata cctgtatggc
      421 cgaccatgtc tgcattggca aaattcaaac agataaaatc agctgttttt tgttctattt
      481 ctgaaacaat ggcatctgtt acttccaggg cactcatttc gggttgcagg tcgtaagtgg
      541 caacttttgg ggacggaacc attattcttt tctccccttc aaaaggattt tcacggccac
      601 cactgaaaaa gaagcttaca tgcggatatt tttctgtttc tgcgatacga atttgtttca
      661 acccgttttt ctccagtatc tcacccaacg tattattgag gttatcggtc tcgaatatta
      721 cctgtacatt attgtatgtt ttatcatatt ctgtcatagt ggtataatgc aacgaaagct
      781 tattcatacc aaactcgggc atatccaatt gtgtaagtac ttgtgttact tcccggcacc
      841 ggtcggttct gaaattgaag catatggcta catccccatt tttaattgta gccagtggtt
      901 gctgtgcctc attcacgatc actgttggtt tgataaattc gtctgttata ttctgggcat
      961 acgaattttc tactgcagca atggcgtctg ttgctttagg gccttcccca tttatcaaag
     1021 tatcataggc cagcttaacc ctttcccatc ttttatcacg gtccattgca tagtagcgtc
     1081 cgcttacgga agcaatttta cccacactat agtttaaatg ctgttgcaat tcaatcatga
     1141 aagcaagtcc acttttcgga tcacaatccc ggccatctgt aaacgcatga ataaatacat
     1201 ctttcagccc ttctgcttta caaacatcaa cgatggcttt taaatggttt atatgcgagt
     1261 gaacgccacc attactgacc aatcccagca ggtggagagg tttgttgttt tcttttgcaa
     1321 aacgtacagc actcagtaat ttctggttct gtgcaaaaga gccatccctt actgctacat
     1381 taattctttg tagttcctgg taaactatcc tccctgcacc caggtttaaa tggcctactt
     1441 cagagttacc catttgtccg tcaggcagac cgacagcctc gccacatgta acaagagtag
     1501 tattagggta ttttgagtaa aggcttttgg taaaaggcac attggcatgc tggatagcat
     1561 cggaggacgc aacttttcct aatccccagc cgtccataat gataagaatt acttttttag
     1621 ctgacatgta atattattaa ggggtaaaac atatttattt cagggaataa ttcctgtccg
     1681 taccctgtaa aactacaccc aaatcagtta tttcccagcc ctattactca aaagatgctg
     1741 atttgtagta ttttagtacc tgttgattgc tgatttgtaa catggcatgt ttttagttta
     1801 gtacttctgt gaaaaccaac aattgatttg atatgaaaca gatatttaca acattacttc
     1861 tttctgctgt tttgatgctt tcttccttta aagcacaaga tggtattgat gaagttatcg
     1921 gcgctttaaa gtcgggtaat gcaactgagt tagcaaaata ttttgatgat acagtagaat
     1981 tgacattgcc tgataagagt gacagttata gcaaagcaca ggcacaactg atcgtgaaag
     2041 attttttcac taacaacggg gtcaaaggtt tcgaattgaa acacaaaggg acatccactg
     2101 gcggttattt tgctattgga acattgcaga ccaattccgg aaatttccgc actaatattt
     2161 ttatgaaaac caagagcgga aaagatgtag ttaaggaaat tcggttccag tcgatagatt
     2221 aaagcgtatt caaccatatt ctaaaatccc cattacatgc aaaatgtaat ggggattttt
     2281 ttgcagtacc ggcatttggc gaaacagtat ttttgcagct atgaattttg atgaacaact
     2341 acatcacctt attgatgagg ccctgaaaga agatgttggt gacggggatc attctacctt
     2401 atcctgtatc ccggccggtg caaaagggaa agcagtgctg aaaataaaac agggtggtgt
     2461 actggctgga atgcaggtgg ccctgaaaat attcagctat aaagaaccag cttcagtttt
     2521 tacatccttt aaagaagatg gtgaagaaat gaagtacgga gaaaaagctt tcgaagtaga
     2581 agcttctgtt cataccattt tgcagtgcga aagacttgtt ctcaattgta tgcagcgcat
     2641 gagtggtata gcaacattga caaaacagta taccggtaaa ttaaaaggct ataaaacgag
     2701 attacttgat acccgcaaaa caactcctaa tttccgttta cttgagaaag aggcggtaag
     2761 aattggcggt ggcgtaaatc atcgtttcgg tttatatgac atgatcatgc tgaaggataa
     2821 tcatattgac tattgcgggg gtatagaaaa tgcgattgaa caggcatttg attatgttca
     2881 aacaaaaaag cctgatctga aaattgaagt ggaaacaagg aacttggatg atgtgagaaa
     2941 ggttattgca attggaaagg ggaaagtatt ccgcatcatg ctggacaatt ttacgcctga
     3001 acaaattgaa gaagcgcttg ggttaattaa gggagatttc gagactgaag ccagtggtgg
     3061 tataaatctt gataatattg aagactatgc aaaaataggg gttgattatg taagtgtggg
     3121 agcattgata caccaggcaa aaagccttga tctgagttta aaagccgtta ttgtatagat
     3181 atgtcaaaaa agaataaacc tgatgcaatg ggatttgtgt atagtaccaa tccaaacttt
     3241 tcctttgaag aagaaagaaa agcaactgaa actcttccac ctgctcaaca aaagctgaaa
     3301 gtgaggctgg atacaaagca ccgggctggc aaggcggtta cattgataga aggttttaca
     3361 gggaaagagg aagacgctca agagttgggc aagaagctga aaagtttctg tggtacaggg
     3421 ggctccgtta aggatggaga gattattgta cagggtgacc aacgggaaaa agtgatgcag
     3481 tggttgttga agaatgggta taaaaatgtc aaaaaaacct gatgcaacag ccctggtctt
     3541 atattattgc cggtatttgc ttttgtatgg caggcatatt cttgtttcag cgtaatgcat
     3601 ttgaggagca taagaaaata cacatgcctt tactgttgat cgttgcaggt attattttag
     3661 tctcaatagg tacagccaag cggttacatc taatagcatg atttttttct ataagaggct
     3721 atataacctg ccagatccga cattcattct ccttcaaaaa tcgggtatag acattctatc
     3781 aatgtacaat cgatgttaaa tttattgatt cctaagtttg tagcctggca tatttaatca
     3841 atttccccat caaatgtatt gtctggagtg tatttaatta ccggcggtta aaaacataac
     3901 ctggcgattg gtctccattt ttatggctta taacttaata aaagcaatat tctttttgaa
     3961 atcttttcca gtaatttcta ccgtattata cccggaagga attccagcca gggttatttg
     4021 tgtacgaaac aggtcagttg atttattgaa ctgaatttgc tttacttgtc tgccgtcatt
     4081 ttgaaatata gttacaatga aattaccggt tgcaggggtg ttaatttcaa tagtaataaa
     4141 ctcactggca ggatttgggt aaagattgac agtgctctca ttatcctcaa ttgtaatttt
     4201 tttggtatca taataggttc ttcggccatt tagatcagtt tgagagagcc ggtaaaaaaa
     4261 cgtacccgat gtaaggccga tgtcttcata attataacta ctgcctgtaa atatattggt
     4321 cccctgaacc tgggctatac tagtaaaatc ttgtccatta gttgaccttt caacagtaaa
     4381 atagttatta ttctgttctt gagaagtacg ccagttaata aaaacactgg tggaagtaga
     4441 ttttactata gaatattcta aaagttctac aggtagaatg atattggttc gttgatgtgt
     4501 taagagccat tcatatacat ttaaagtccc attttctctc caactgggat tataagttgt
     4561 tgtccaggca tcatgcccat ttacattaaa tattgttttt ttaggtgcaa tgctagaggt
     4621 gactggggca ttttgataat gggtaatata gtcatttgta taagacactg gtactgtctg
     4681 atctccgttg ttatgcgtag cccatacagg taaattagca gctgccatag tgaatgctct
     4741 gctgctttca ggccatgaag cgccacaaac gggtaacaaa gctgccagtc tatttgcata
     4801 aactgaatta tcacctgcgt attcccaggt ggctcctcca cccatactta ggcccgtcaa
     4861 atatattcta tttatattaa ctctgtaatt ctgagaaacg tagttaatta tattgttgat
     4921 gtcattcgga gttggccagt cgataaattg tggagatatt acaataaaac tgaatgtttg
     4981 accattaact gtaaaagaag agggaaaatt accctggtta ataatctttg gaggaccatt
     5041 gaataaaacc cttggtaatt gtgccgcact gccatttcct aattcaccta ggccatgtag
     5101 aaaaatcaat aatggataag ttgcctggtt tccaggagta gaataccctg ttggtaaata
     5161 ctcatagtat ccattactat ttgcaatcat cgaaatatac ctggcagttt gcacttgtgc
     5221 atttactgat gctgatatca gaaatagaat cagaaataag tttttaaaat tccttaacct
     5281 aagggaatgc atatattaaa ttttgaagtg aaataaatta tcagtggaaa attattggtc
     5341 agttgatctg tattgctctt tttattgttt gcgggtatga taaaaggcgt cattattaga
     5401 ttaaagatga gctgtattga aggtataatt cttacacatt cataaaaaga acttatatga
     5461 ttcacacatt taaaagtaaa tggatgtaag cccgaatagc ccttttactt actaaccgtt
     5521 ttttagaata taattgaacc tgctaaacta ttataggata taaaaattta gcgaatcaga
     5581 tcattttagt actttttaaa at
//
LOCUS       DAORTI010000183         5587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17432_length_5587_cov_7.281815, whole genome shotgun sequence.
ACCESSION   DAORTI010000183 DAORTI010000000
VERSION     DAORTI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17432_length_5587_cov_7.281815"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..89)
                     /locus_tag="PLO70_15375"
     tRNA            complement(2..89)
                     /locus_tag="PLO70_15375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Ser,seq:gga)
     gene            complement(130..588)
                     /locus_tag="PLO70_15380"
     CDS             complement(130..588)
                     /locus_tag="PLO70_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQZ75901.1"
                     /translation="MPDKKTNRGYLASSLGCHCPRCREGKLFKNPVTLSFKKNLEMNK
                     QCPVCSQATEIEVGFYYGTSYVSYALTVALSVSTLVAWWVLIGLSTEDNRFFYWMGSN
                     ALFLILLQPWIMRVSRSLWISWFVKYDPDWEHKKPEDVSERLNAEQANNW"
     gene            complement(591..914)
                     /gene="trxA"
                     /locus_tag="PLO70_15385"
     CDS             complement(591..914)
                     /gene="trxA"
                     /locus_tag="PLO70_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQZ75902.1"
                     /translation="MQTANKKESFGEIINGSTPVLVDFSAEWCGPCRMMKPILQQLHQ
                     RMGEKIRIIKIDIDKSPAAAQTYNVQSVPTLIIFQNGKMLWRQSGVVQATQLEKIIEQ
                     HTTNN"
     gene            complement(898..1107)
                     /locus_tag="PLO70_15390"
     CDS             complement(898..1107)
                     /locus_tag="PLO70_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HQZ75903.1"
                     /translation="MKSWVLNNKLLIAGVVLGAIGGYLYWQQIGCDSGTCAITSKWHN
                     STAYGALMGGLLFSMFKKEKNANSK"
     gene            complement(1085..1339)
                     /locus_tag="PLO70_15395"
     CDS             complement(1085..1339)
                     /locus_tag="PLO70_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75904.1"
                     /translation="MNLIGRLKSGWTSLKFIRVGLGSLILYSAIESGQVSGIILGGLF
                     TIFSLFTDGVCCAGGGCYTPVNKNNSSTPENIDYEELGSK"
     gene            complement(1455..2651)
                     /locus_tag="PLO70_15400"
     CDS             complement(1455..2651)
                     /locus_tag="PLO70_15400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQZ75905.1"
                     /translation="MPTHHQVVIIGGGNAGISVAAKLLLKNKQLDIAIIDPADKHYYQ
                     PAWTLVGSGVFDIKKTERNEADVMPKDVKWIKQKVTGLTPESNTVQLGNGEEISYDYL
                     VVAPGIQINWQEIKGLKETLGKNNVCSNYSFEHAPYTFECFKNFKGGKAIFHNPQTPV
                     KCGGAPHKIMYMAADYFRRHGLLDKADIQYWSGAAKLFSVPKYEKTLLKVCERADIKL
                     NFMVQLVEIDGPGKKAKFIGIGDTNKDKEFWVEFDMIHVTPPQSAPDFIRTSSLANAA
                     GWVDVDKHSLQHVKYANVFSLGDASGLPISKTGAAIRKQAPVVVENLLAAIHHQPLTG
                     KYNGYTSCPLVTGYGKLVLAEFDYDNNPQETFPFDQSKERWSMYQLKKRILPWLYWHK
                     ILHGKM"
     gene            3001..4425
                     /locus_tag="PLO70_15405"
     CDS             3001..4425
                     /locus_tag="PLO70_15405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ75906.1"
                     /translation="MKVEQIYTGCLAEAAYYIESNGEVAIIDPLRETKPYIDKAERNG
                     AKIKYILETHFHADFVSGHIDLAKKTGATIVFGPTAAPAYDVHVANDGEELALGKVKI
                     KVLHTPGHTMESTTFLLVDENGKEHAIFTGDTLFIGDVGRPDLVQKVKAEITPQILAG
                     YLFDSLRNKIMTLPDEVIVYPGHGAGSACGKNMSKETTDTLGHQKMTNYALRADMSKE
                     DFIKEVTEGLVEPPQYFPMNVLMNLKGGLESIDEVIAKGKHPLTAKEFQTVWETMEAL
                     VLDTRSKDSFATEHIPGSISIGIDDNFAPWVGTLVTDLKMPILFIADKGREEEVVIRL
                     SRVGYDNTVGYLDGGITAWKEAGFTTDSIKEISAEDFATLYEMDKQHINLLDARRKSE
                     FDSEHLVGAENFPLDFINHNMSHLDRNKKYFIHCAGGYRSVIMASILKARGFEHLVNI
                     QGGYNALSKTGLKKTHYVEPITML"
     gene            complement(4460..4930)
                     /locus_tag="PLO70_15410"
     CDS             complement(4460..4930)
                     /locus_tag="PLO70_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQZ75907.1"
                     /translation="MILDKTDVQILELLQADALLTNKELADKLGKTITPIYERIRRLE
                     NEGYITRYCAIVDKNKIGKSLVAFTNVQLKQHAQAILLSFEKAIVKFEEVMECYHMTG
                     VYDYLLKIVVTDMAAYQDFIVNKLARLTDIGTVQSSFVMTEVKHETAYKIKPAR"
     gene            5082..>5587
                     /locus_tag="PLO70_15415"
     CDS             5082..>5587
                     /locus_tag="PLO70_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQZ75908.1"
                     /translation="MSHTKFSPETQMLGYGYKPELSEGAVKCPIFQTSTFVFKSAEEG
                     KAFFEMAMGKPGHEGEEMGLIYSRINNPDMEILEERLKLWDGGEAAAAFSSGMSAIAT
                     TLVTYLTPGDVLLYSNPLYGGTHKFIHHILTHFGVEVIGFRAGQDLKEVEKILVESGR
                     ADKLKMIY"
BASE COUNT         1658 a         1076 c         1133 g         1720 t
ORIGIN      
        1 gcggagactg agggattcga accctcgata ccctttagag gtatacacac tttccaggcg
       61 tgctccttca accactcgga caagtctcca ttccttttga aaagggcagc aaaaataggg
      121 attcttgtct taccagttat tagcttgttc tgcatttaat ctttcagata catcctccgg
      181 ctttttgtgt tcccaatcgg gatcatattt cacaaaccag gagatccaga gactgcgact
      241 gacccgcatg atccagggtt gtaataaaat gagaaaaaga gcattggagc ccatccagta
      301 aaagaaacgg ttatcttccg tagaaaggcc aatcagaacc caccatgcaa ccaatgttga
      361 cacgctaagt gcaacagtta aggcatagct tacatagctg gtaccataat aaaaacctac
      421 ttcaatctct gtggcctggc tgcacacagg gcattgttta ttcatttcca ggtttttctt
      481 aaaacttaat gttactggat ttttgaataa ttttccttca cgacaccttg ggcaatgaca
      541 acctaaagaa gaagcgagat acccacggtt tgttttttta tcaggcattc ttagttattg
      601 gttgtatgtt gttcaatgat tttttctaat tgtgtggctt gtacaactcc ggattgccgc
      661 caaagcattt tcccgttttg aaaaataata agtgttggca cactttgtac attatatgtt
      721 tgtgcagctg ccggactttt atcaatatca attttaatga tgcggatttt ttctcccatt
      781 cgttgatgta attgctgcag gatcggcttc atcatccggc agggcccaca ccattcagca
      841 gaaaaatcaa ccagcaccgg ggtgctgcca ttgataattt caccaaagct ttctttttta
      901 tttgctgttt gcattttttt cttttttgaa catactgaac agtaatcctc ccatcaatgc
      961 tccgtaagca gtactgttgt gccacttaga agtgatagca caggttccgc tgtcgcaacc
     1021 aatctgctgc cagtagagat aaccaccaat ggcgcctaac actactcctg caatcagtaa
     1081 cttgttattt agaacccaac tcttcatagt ctatattttc aggggttgat gaattatttt
     1141 tattaacagg tgtatagcag ccgcccccgg cacaacatac accatccgta aatagggaaa
     1201 aaatggtaaa caatcctccc aatattattc cggatacctg gccggattca atggcagaat
     1261 acaaaattaa gctacccagt ccaactctta tgaactttaa cgatgtccat ccgcttttaa
     1321 gtcttccaat aagattcata accagaattt tataccacaa acctcccaat ctccgataac
     1381 aatatgcgtg acattagtta caaaaaagcg gcctgttcag gtgccgcttc ggttatttca
     1441 tttatattat ttgattacat cttgccatgc agtattttat gccaataaag ccaggggaga
     1501 attctttttt tgagctggta catactccac ctttctttgc tctggtcaaa ggggaaggtt
     1561 tcctgcgggt tgttgtcata atcaaattct gccaaaacca gttttccata cccggtaacc
     1621 aacggacaac tggtgtaacc attatatttc ccggttaatg gctggtgatg gatagcagcc
     1681 aatagatttt ctactacgac aggtgcttgt ttacgtattg ctgcacctgt tttgctgatt
     1741 ggcaaacctg atgcatcgcc caatgaaaaa acgttagcat attttacatg ttgtaaactg
     1801 tgtttatcaa catccaccca accggcagca ttggccagcg aacttgtcct tataaaatcg
     1861 ggagcacttt gcggtggggt gacatgtatc atatcaaatt ctacccaaaa ctctttgtct
     1921 ttgtttgtat ctcctatacc tataaatttt gcctttttgc cgggcccgtc aatttccacc
     1981 aattgtacca tgaaattgag tttgatatct gctctttcac aaaccttaag caaggttttt
     2041 tcatacttag gaacagaaaa tagtttggca gcaccgctcc agtattgaat atctgctttg
     2101 tctaataagc catgcctccg gaaataatcg gcggccatat acatgatctt atgtggggca
     2161 ccgccacatt ttactggtgt ttgcgggtta tgaaaaattg ctttacctcc tttgaaattt
     2221 ttaaaacatt caaatgtata gggtgcatgc tcaaaactat agtttgagca aacattgttc
     2281 ttgcccaatg tttctttcaa tccttttatc tcctgccaat tgatttgaat tccgggggct
     2341 actaccaggt agtcataact aatttcctct ccattaccca gttgaacagt attcgactca
     2401 ggggttaacc cggttacttt ctgttttatc cattttacat ctttgggcat tacatctgct
     2461 tcatttcgtt ctgttttttt tatgtcaaag actccgctgc ccactaaagt ccatgccggc
     2521 tggtaatagt gtttatctgc tgggtcaata atagcaatgt caagttgctt gttttttaaa
     2581 agaagctttg cagctacaga gataccggcg tttccgccac cgatgataac tacctggtga
     2641 tgagttggca ttttaattga atttaatact gccagttaca gtaataaaat aaatcattgc
     2701 gtgactttaa tcacccttca acctaaagct attgaggaat agggctttat caaatttttt
     2761 ttaatgggga gtatataaga aaaaagctga atgatgaatg atcttcatca ggcgaataac
     2821 tgataaaatt cagttgaaat tgtaacccaa cacactaatc gaaaaacttt atgataccat
     2881 ttttgaagca gaaattgttg attcgtgtcc gggtgggttg accaatagta aatctataat
     2941 ggcaactcac ttttgtaaac gattatttgt aattgaaaat ataaaataaa acaattgatt
     3001 atgaaagttg aacaaatcta taccggttgc ctggctgaag cagcttatta catcgaaagt
     3061 aatggtgaag tggcaattat tgatccgttg cgggagacca aaccgtatat tgataaagct
     3121 gaacgtaatg gtgctaaaat aaaatatatc ctggagacgc attttcatgc tgattttgta
     3181 agcggccata tcgatttggc aaagaaaaca ggagccacta ttgtatttgg tcctacagca
     3241 gcccctgctt atgatgtgca tgtagcaaat gatggggaag aacttgcatt ggggaaagta
     3301 aaaataaaag tgctgcatac tccgggacat acaatggaat caaccacttt tctgctggta
     3361 gatgaaaacg gaaaagaaca tgccattttc accggggata ctttatttat tggtgatgtg
     3421 ggccgtccgg acctggtaca aaaagtgaaa gctgagataa ccccacagat attggcaggg
     3481 tatctttttg attcattgcg taacaagatc atgacattgc cagatgaggt aattgtttat
     3541 ccaggacatg gagccggaag tgcttgcgga aaaaatatga gcaaagaaac aacggataca
     3601 ttgggccatc agaaaatgac caattatgca ctgcgggcag acatgagtaa agaagatttc
     3661 atcaaagaag ttacagaagg gttggtggaa ccaccgcaat atttcccgat gaatgtattg
     3721 atgaacctca aaggcggatt ggagagcatt gatgaagtga ttgcaaaagg taagcatcca
     3781 ctgacagcaa aagagtttca aacagtctgg gaaactatgg aggcgttagt tttggatacc
     3841 cgcagtaagg attcttttgc tacagagcat atacctggat ccatttctat tgggattgat
     3901 gacaattttg caccctgggt aggtacattg gttactgacc tgaaaatgcc aatacttttt
     3961 atagctgata agggaagaga agaagaagta gtgattcggc tttcaagagt tggatatgat
     4021 aatacagtgg gttacctgga tggtggaatt acagcctgga aagaagctgg gtttactact
     4081 gattctataa aagagatcag tgcagaggat tttgccactt tatatgaaat ggacaaacag
     4141 catataaacc tgctggatgc caggcgtaaa agtgaatttg attcagaaca tcttgtagga
     4201 gctgagaatt tcccattgga ttttatcaat cataatatgt cacatttaga taggaataaa
     4261 aagtacttta ttcattgcgc cggtggttat cgttctgtaa ttatggcttc tattttaaaa
     4321 gccaggggct ttgaacacct ggtaaatatt caaggtgggt ataatgcatt aagtaaaaca
     4381 gggcttaaaa aaacacatta tgtagaacca atcacgatgt tgtaaggaaa gcgaaaatga
     4441 atgtaggtag aaaccggcct taacgggccg gttttatttt gtacgcagtt tcatgtttta
     4501 cttctgtcat tacaaaactg ctttgtaccg taccaatatc agtaagcctg gccagtttgt
     4561 tgacgataaa atcctggtaa gcagccatat ctgtcactac aatcttcagc aggtaatcat
     4621 atacaccagt catgtgatag cattccatta cttcttcaaa tttcacaatg gctttttcaa
     4681 agctcagtaa aatggcttgt gcatgttgtt tcaattgtac gttggtaaat gccaccaggc
     4741 tcttcccgat cttattttta tctactattg cacagtaacg ggtgatataa ccttcgtttt
     4801 ccaggcgacg aattctttca taaatggggg tgatcgtctt tcccagttta tccgccaatt
     4861 ctttattggt caacaaggcg tctgcttgca gtagttccag tatttgaaca tctgttttat
     4921 ctaaaatcat ttaggataat ttatctaaaa ataactacta atatagataa aaaatctttt
     4981 ttaatagcat ttcgttagaa aaatagattt gttttctaat gtaactaaag tcattgtaac
     5041 tgacaatatt tcttgtcaat ttagtgttca aattcaagaa tatgagtcac acaaaatttt
     5101 ccccggaaac ccaaatgctg ggctatgggt ataaaccaga attaagtgaa ggtgctgtaa
     5161 aatgtcccat cttccagacg tccacttttg ttttcaaaag tgcagaggag ggtaaagcat
     5221 tttttgaaat ggcaatgggt aagcccggac atgagggaga agaaatgggt ttgatctaca
     5281 gccgcatcaa taatcctgat atggaaatac tggaagagcg attgaaattg tgggatggag
     5341 gagaagcagc ggcagctttt tcaagtggta tgagtgctat tgcaactacc ctggttactt
     5401 atcttactcc gggggatgta ttgctgtata gtaacccatt gtatgggggc acacataaat
     5461 ttatccatca tatccttact cattttggtg tggaagtgat tggtttccgg gcagggcagg
     5521 atttgaaaga agtggaaaag atattagttg aaagtggccg tgcggacaaa ttgaaaatga
     5581 tttatat
//
LOCUS       DAORTI010000184         5532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_17679_length_5532_cov_6.034508, whole genome shotgun sequence.
ACCESSION   DAORTI010000184 DAORTI010000000
VERSION     DAORTI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17679_length_5532_cov_6.034508"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLO70_15420"
     CDS             <1..275
                     /locus_tag="PLO70_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75909.1"
                     /translation="QYIFWYGGFIFLTVYALTELMDRNPWALFWEAARSGLGLAFLLQ
                     QNDWFGSVTYFSPVMYALSAYFIFSFLVTAWFVVKHRKEDALEMAA"
     gene            773..1162
                     /locus_tag="PLO70_15425"
     CDS             773..1162
                     /locus_tag="PLO70_15425"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HQZ75910.1"
                     /translation="MFTIEDIKIAHSKVKSGADFPGYIKDIKALGVMLYETYVMDGHT
                     DYYGVDNYKTATTAKYEVLTIADEANAAQFTAYLKAHQQGKTDYSTFCNDCAKSGIEK
                     WVVDVARMTCTYYDTAGNEMLAEAIPQ"
     gene            1262..4738
                     /locus_tag="PLO70_15430"
     CDS             1262..4738
                     /locus_tag="PLO70_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HQZ75911.1"
                     /translation="MLALFAGCFLFIQGQAQQTLKSSYSNRFETTSSDEVFYTIKSVN
                     RINEDSAVITIHYGKQDGIFSDAEANVMSSHNSGSGSTRETVVYMGPAKILSLGDTLT
                     TLSVNLYKKFKKEKLLPGDMVGIASYTVKNSAGDIFHHLATVDVLFLDNSRIEIKSKR
                     EILLNTDPDFERKLLEAYTKEILNFYEDLKEYTGETFIKRNDEGPFKGMNMIEVFKNT
                     TMNDLRAFFQFVKQFPGKYMGNKWKINETYATWVLNNAPLSDLNKQWLIPALQASRSA
                     TEFARKNKHYIDADSLILWSDHVFKLQGESKLDEALQFCDNLIAVAKELKFTKAEYEF
                     YYTRSYLTDAAGNKKEAIAEALKALTYDKENINYAYQLASLYGKNEEFDKCFKLLEDL
                     MKKLPGNVNVMGNYGWYKLAAGKVKEAAPYCKAAYEGNPGSVAFTVNYGHTFLLTGNL
                     DSARYYYLKTLENCSTPNDYYEGPKTDFDLFFKKGWERKNVAEMADWMEQQFNEKYLA
                     VTKGNDVWNEAKKQYDNKKYLKAIPHWLTYITLFDKVKEPPLYSIHNAYNWIGSSYSR
                     AKVYDSARHYYMMALKMAKDTLADMRNVTTTKDNDDLVSDYERLYSLSVTAGNTADAE
                     RYKMLLDAETQKVTELFTRATLHIISLGAAGSAKDMKTAEFVYKNFSSLKNENDKAGH
                     MQLLKGNALTRDKLISSLEELRAKSKPEDIFIFYFAGNTITANNQSYLSFNDKDSVQG
                     RISVLEFMNAIDLVYAHKKMIISDKPSAPLLSLITSTYSNAGKNASEIIFLSPGIETP
                     EQDNGISLFTNQLTITLKELQEKDKFSAKDFVDKASFVLGRGQYYFPVLSFSFGKDFL
                     LYENKSAIQPTDNTLAVNNTRGLDLGASDVTDADNVSGPQKNYALLFATDIYKDGGFN
                     RLVNPIYDAEALAKLLREEFGFTVTLVKNATLDQMETTLSKFRDDINYGPNDQLFLFF
                     AGHGLYYEKIKMGFLVASDSKVDDQNFKTYLSYNDLGSKYLKNINCNRIFVTLDACFA
                     GSFFDNSTFRAGPNDADVKNLAALKRMATKQRFYKGISSGGKQYVEDGKQGQHSPFAS
                     KFIEVLSKKALSKNFVTADEIIGEIKSNPPGASAVCEGKFHYSDPQSHFIFELKNNQK
                     VSDVKQDTMKTK"
     gene            complement(4744..>5532)
                     /locus_tag="PLO70_15435"
     CDS             complement(4744..>5532)
                     /locus_tag="PLO70_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HQZ75912.1"
                     /translation="SSVNPVFLLPEKMKTAAAVIAKKYAGSITLGVGQFCTNPGLIIG
                     IDNDDLQNFINVLGEEIKQVSPAEMLNTGIFKNYVEKRENSLAQKDVEVKGVSEKQPL
                     TNQGIATVASTTAKAFIENPLLHQEVFGPYSIIIRCTDISEMLHVAKHIEGQLTATFI
                     ATEDDVKQNEELVEAVKNICGRFIINSVPTGVEVCLSMQHGGPFPASTDARFGAVGAD
                     AIKRFARPIAFQNWSNDLLPDELKNENSLSIWRTVNNELTKAAV"
BASE COUNT         1754 a         1028 c         1145 g         1605 t
ORIGIN      
        1 ctcaatatat tttctggtac ggtggtttta tttttcttac ggtctatgca cttacggagt
       61 taatggatcg taacccatgg gctttgtttt gggaagctgc ccgcagcggt ttgggtcttg
      121 catttttgtt gcagcagaat gattggtttg gcagcgtcac ttatttttct ccggtaatgt
      181 atgcactcag tgcttatttt atattttcat ttttagttac cgcctggttt gtagtaaaac
      241 accggaagga agatgcgttg gagatggcgg catagttttc tctgtcattc cgggcttgac
      301 ccggaatctt tctcaaaatg attattagcc cagtttacct accggaattt tatatggatg
      361 catgcatctg tactactatc atcgtccctt gcacagttta ccctgaggaa cgaaaggcaa
      421 tattcagggt tctgtaattc tattcagcat taaagtataa tacaaatacg aataacgacg
      481 attgcaacaa cccagcacac aaagctatct aacactcgtc tgggaaatga aacaaatgtc
      541 agtgtaccaa aaatgctgca agcaattgtt aatgccgtag ccggctgtaa taaatcagct
      601 ttactatttg gctgtaatgc aatacagtca atagtttcag cccaatgtta tttaggttaa
      661 taaaaagagt agctacgtta atacaggaaa ataaaaaaat ctaaatagag ttttgggagt
      721 tttatcttta gcatccattt atttgattga cataactaat aaaacaaaca agatgtttac
      781 aatagaagat attaaaatag cacatagcaa agtaaaatca ggcgcagact ttcctggata
      841 tattaaagat attaaagcac ttggtgtaat gctttatgaa acttatgtaa tggatggcca
      901 cactgactat tatggagtag ataattacaa aacagcaaca acggctaaat atgaagtact
      961 gacaattgca gatgaagcga atgcagcgca gtttacagca tatctaaaag cacatcagca
     1021 aggcaaaaca gattactcaa ccttttgcaa tgattgcgca aagagtggta ttgagaaatg
     1081 ggtggttgat gtggcaagaa tgacttgtac ttattatgat acagcaggga atgaaatgct
     1141 ggcggaagca attcctcaat aatgaacatc tgcaacaaat acccaacacc acgtatacaa
     1201 aagggctgaa atatttgtac ttttcggtat gtttttttta ccaccagtaa aagctttgcg
     1261 aatgctggca ttatttgccg gttgttttct ttttatacaa gggcaggcac agcaaacttt
     1321 aaagagcagt tatagcaacc gctttgaaac caccagcagc gatgaggttt tttatacgat
     1381 aaagtcagtg aaccgtataa acgaggattc ggccgttatt actattcatt atggaaagca
     1441 ggatggcatt ttttcagatg cagaagccaa tgtgatgagc tcacacaata gcggtagcgg
     1501 atcaactagg gaaactgtgg tatatatggg ccctgccaaa atactaagcc tgggtgatac
     1561 actgactaca ctttctgtaa acctgtataa gaaatttaaa aaagaaaaac tgctgcctgg
     1621 tgatatggtg ggcattgcat cttatactgt aaagaacagt gccggagata tattccatca
     1681 tctggccacg gtggatgtgc tgtttcttga taactcaaga atagaaataa aaagcaagag
     1741 agagatactg ctgaacaccg atcctgattt tgaaaggaaa ctattggaag cctataccaa
     1801 agagatactt aatttttatg aagatttgaa agaatataca ggggaaactt ttataaaacg
     1861 aaacgatgaa ggacctttta agggcatgaa catgatagag gtttttaaga acacaacgat
     1921 gaacgacctc cgtgcctttt tccagtttgt aaaacaattt ccgggtaaat acatgggcaa
     1981 caaatggaag ataaatgaaa cctatgctac ctgggtatta aacaatgcac cgctaagtga
     2041 cctgaataag caatggctga tacctgccct gcaggcaagc aggagtgcaa cagaatttgc
     2101 ccgtaagaac aaacattata ttgatgcaga ctccctgata ctttggagtg atcatgtttt
     2161 taaactacaa ggtgaaagca agctggatga agctttacag ttttgcgata atctgatagc
     2221 cgtggcaaag gaattgaaat tcaccaaagc tgaatacgaa ttttattata cacggtcata
     2281 cctgacggat gcagcgggta ataagaaaga agcaattgct gaagcactga aagctcttac
     2341 atatgataag gagaatatca actatgctta ccagcttgcc agcctgtatg gaaagaatga
     2401 agagtttgat aaatgtttta aactgctgga agacctgatg aaaaaattgc ccggtaatgt
     2461 gaatgtgatg ggcaactatg gctggtataa attggctgcc ggaaaagtta aagaagcagc
     2521 accttattgc aaagcggcat acgaaggcaa ccccggcagt gtagcattta ctgttaacta
     2581 tggtcataca tttttattga ccggcaacct ggacagtgcc cggtattatt atttgaagac
     2641 attggagaac tgttcaacac ccaatgatta ttatgaaggg ccaaaaacgg actttgatct
     2701 tttctttaaa aaaggatggg aacgaaaaaa tgtggcggaa atggccgatt ggatggaaca
     2761 acagttcaat gaaaaatacc ttgcggtaac caaaggcaat gatgtatgga acgaagcgaa
     2821 gaaacaatat gataataaga aatacctgaa ggctataccg cactggctta cttatattac
     2881 tttgtttgat aaagtgaaag agccaccgct gtattctatt cataatgctt ataactggat
     2941 cggttcttct tattcccgtg ccaaagtata tgacagtgcc cgccattatt atatgatggc
     3001 tttgaaaatg gcgaaggata cactggcaga catgcggaat gtgacgacca ccaaagacaa
     3061 tgatgacctg gtgagtgatt atgaaagatt atacagcctt tctgttactg ccggcaatac
     3121 tgctgatgcg gaacgatata agatgctgct ggatgcagag acacaaaaag taacagagct
     3181 gtttacaaga gcaacgcttc atattatatc gctgggtgct gctggttctg caaaagatat
     3241 gaagacggct gagtttgttt acaagaattt ttcttcttta aagaatgaaa atgacaaggc
     3301 cggccatatg caattgctga aaggtaatgc actgacccgt gacaaactca ttagcagcct
     3361 ggaagagcta agagcaaaat caaaaccgga agatattttt attttctact tcgccgggaa
     3421 tacgattact gccaacaacc aatcctacct cagctttaat gataaggatt cggtgcaagg
     3481 aagaatatct gtgcttgaat tcatgaatgc gattgattta gtttatgctc ataagaaaat
     3541 gatcatttct gataagccat ccgcaccgct tctttcactc atcacttcca cgtacagcaa
     3601 tgcaggtaag aacgcatcgg aaataatctt tctttcgccg ggaatagaaa caccggaaca
     3661 ggataatggc atttcccttt tcaccaacca attaacgatt acactgaaag aattacagga
     3721 aaaagacaag ttctctgcaa aggactttgt tgacaaggct tcttttgttt tgggaagagg
     3781 acaatattat ttcccggtgc tatccttcag ctttggcaaa gattttttgt tgtatgaaaa
     3841 taaatcagca atacaaccga ctgataacac actggccgtt aataatacaa gaggtcttga
     3901 cctcggtgca tctgatgtaa ccgatgctga caatgtaagc ggaccgcaaa aaaattatgc
     3961 attgttgttc gccacagata tttataagga tggcgggttt aacaggttag tgaatcctat
     4021 ttatgatgca gaggcattgg ctaaattatt aagagaggaa tttggtttta cagtaactct
     4081 tgtaaagaat gctacgcttg accagatgga aacaacgctg agtaaattca gggatgatat
     4141 aaactacggc ccgaatgacc agttgttcct tttctttgca ggtcatggtt tatactatga
     4201 aaagataaag atgggattcc tggtggcttc tgattcaaaa gtggatgacc agaattttaa
     4261 aacctatctc tcctataatg atcttggcag caagtacctg aaaaatatta actgcaaccg
     4321 catctttgta acactggatg cctgcttcgc cggttctttc tttgataaca gtactttccg
     4381 tgccggaccg aatgatgctg atgtaaaaaa tcttgccgca ttaaaaagaa tggccactaa
     4441 acaacgtttc tacaaaggca tttcatccgg cggtaaacaa tatgtggaag atggaaaaca
     4501 aggacaacac tctccttttg ccagcaagtt tattgaggtg ctttctaaaa aagcattaag
     4561 caagaatttt gtaacggctg atgagatcat cggtgagata aaaagtaatc ctcccggtgc
     4621 ttctgctgta tgcgagggga agttccacta cagcgatccg cagagtcatt ttatttttga
     4681 attgaagaac aatcagaaag tttctgatgt aaagcaggac accatgaaaa cgaaataaca
     4741 agtttacaca gcagcttttg tcaattcatt attcaccgtt ctccagatgc ttaaagaatt
     4801 ttcatttttc aattcatccg gcaataaatc attactccag ttttgaaaag caatcggtct
     4861 tgcaaaacgt tttatcgcat ctgctcctac tgcgccaaat cttgcatctg tacttgcagg
     4921 aaaaggtcca ccgtgctgca tacttaaaca aacttctaca ccggtaggca cactgtttat
     4981 tataaaacgt ccgcaaatgt tcttaacagc ttctactagt tcttcgtttt gttttacatc
     5041 atcctctgtt gcaatgaagg ttgctgtcaa ttgtccttct atatgctttg ctacatggag
     5101 catttcactt atatcggtgc atcgaatgat tattgaatat ggaccaaaca cttcctggtg
     5161 taataatgga ttttctataa atgcttttgc tgtagtacta gcaactgttg ctattccttg
     5221 gttagtcagt ggttgttttt ccgatacgcc ttttacttcc acatcttttt gtgcaagaga
     5281 gttttccctc ttctccacat agtttttaaa gatgcctgtg tttagcattt cggcggggct
     5341 tacttgtttt atttcttcgc ccaacacatt gatgaaattt tgcaagtcat cattatcaat
     5401 gccgattatt aatccgggat tggtacagaa ctgaccaaca cctaatgtga tggagcctgc
     5461 atattttttt gcaataacag cggcagctgt tttcattttc tccggtaata aaaaaacagg
     5521 attgacgctg ct
//
LOCUS       DAORTI010000185         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18370_length_5405_cov_4.640935, whole genome shotgun sequence.
ACCESSION   DAORTI010000185 DAORTI010000000
VERSION     DAORTI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18370_length_5405_cov_4.640935"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLO70_15440"
     CDS             complement(<1..423)
                     /locus_tag="PLO70_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75913.1"
                     /translation="MKNLIILACFFAYTITAQAQNNPPIGSQKGVKEKFIRIKKGLPA
                     IKKNLTEQDEMWTDVRNVKFEMGDGTILYEEDEEEDNIDQYLKIRFSQSYFSGTVTDY
                     QYYYKILVAMIKEVFGPGFEASSTEKEKKWSTTIFEKGK"
     gene            complement(482..2257)
                     /gene="argS"
                     /locus_tag="PLO70_15445"
     CDS             complement(482..2257)
                     /gene="argS"
                     /locus_tag="PLO70_15445"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQZ75914.1"
                     /translation="MSIVSQIKPLVIKTLEELYGQAFNETAITISSTKPEFEGDYTLV
                     LFSFVKQLKKSPEQLGTEIGEALIQSDPGLFSGYNVIKGFLNLTVTDRFWTTVLQSNY
                     ANTKFGFKPANGQKVMVEYSSPNTNKPLHLGHLRNNFLGWSVAQMLRESGYEVIKSCI
                     VNDRGIHICKSMIAWQLFADGATPQSTHTKGDHFVGEYYVKFNDEYKRQTEELIAGGM
                     KKEEAEKEAPIMKATQQMLLDWEAGNPAVMELWKKMNGWVYAGFDVTYKRIGSDFAKM
                     YYESETYLPGKKFVEEGLMQGVFFKKEDGSVWIDLTAEGLDEKLVLRKDGTSVYITQD
                     LGLAQQKYEEFNYNQSIYVIADEQNYHMKVLKLILQKLGKPYADGIYHLSYGMVELPT
                     GRMKSREGTVVDADDMIEEMVNVARKKTEELGKVKDFTEAELKELYDTIALGAIKFFL
                     LRVDPKKKMIFNPEESIDFHGFTGPFVQYTHARIKSILRKQTAGKQLQTTSPLHKLEK
                     DLIVSLEQYPVIIEQAVAEHNPSVLAIYAFNLAKSFNTFYTELSVMNAETEEKKELRL
                     QIAEMTANVIASAMSLLGIKVPERM"
     gene            2333..2848
                     /locus_tag="PLO70_15450"
     CDS             2333..2848
                     /locus_tag="PLO70_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQZ75915.1"
                     /translation="MRRHIHNARDFILPIIDFFYPPFRRLMNLQTFRYAASGGGNVML
                     GFIIYSLSYTYLFFGRDFNFGFYAFKGHSAALFVSFCVTFPIGFFMSKYVVFSDSKMK
                     GRIQLFRYFMICLFNLAMNYLLLKIFVERLNVHPILSQVITVAIVVVFSYLAQRNFSF
                     KVSAIDDELKD"
     gene            complement(2838..4268)
                     /locus_tag="PLO70_15455"
     CDS             complement(2838..4268)
                     /locus_tag="PLO70_15455"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HQZ75916.1"
                     /translation="MKGKGILVLICLLGVFSSYSQKINPANPDLSPGNFKLDSLPNSE
                     INIPIQIDLKPVYAMAEKSVDTLFTSPGYPEGWVQEACDTRYKYIFRRSKLQMQASGM
                     SLNLGFTGYYKIIGSTRVCANGVVLSPWTPPCRCGFNEPERRVNVSFTNSLTLMTNYK
                     VKLAINRNEPQPIDKCEVCFWGQNITKQVMKGLVAELDAAKKDLDKNYGTVDLKPQFQ
                     KIWDQLNKVYDIYGLGWLKINPKGVHINRLYAQNDSLNIFLGLSAKPVVSFEKPAEES
                     SWVPHINNAGSRKGFSIFLDAMLNYDSLSSILNMQVAGMSFDFKKAFVRKKFVIDSCR
                     IYGGGFEKLIIKINFSGTNQGIVYLVGKPVYDSASRTIAITNIDFDIKSKNVLLGSAD
                     WLFDKKITKEITRYARFELGAYIDTAKTSFNQQLNQSWMKGISSYGNINDIQLIGIYP
                     MQQHLVIRSNCEGNLSVKVDSGSFSL"
     gene            complement(4409..5092)
                     /locus_tag="PLO70_15460"
     CDS             complement(4409..5092)
                     /locus_tag="PLO70_15460"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQZ75917.1"
                     /translation="MATSISDKLRIKPQYNLLTLNAPADFKKGLLGLPEGVKLSDSGK
                     NYNQVHWFVLSKAQLEKEMKKVMKLVLRQAQDNKPDVTVWVYYPKGSSKIQTDLTRDK
                     GWDCLLAESDKLTWISLISFNDTWSVFGFRAKTEADKNKEAKGKPEREIFNWVNPKTK
                     EIKLPDDLAAALKKNKKEAAYFDTLSFTNKKEYIEWIVTAKREETRKERVKGTVERLG
                     KKWKNPRNL"
     gene            complement(5123..>5405)
                     /locus_tag="PLO70_15465"
     CDS             complement(5123..>5405)
                     /locus_tag="PLO70_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQZ75918.1"
                     /translation="CQTGKGMLMQGEGVQGLSSSFLVAGANSLIVSLWSVADKSTSLF
                     MQEVYKLVNKDGLTFKAAIRQVKQYFLEGKFGEEYRKPYYWAPFIYIGN"
BASE COUNT         1366 a         1195 c         1000 g         1844 t
ORIGIN      
        1 tttgcccttt tcaaagattg tcgtactcca ttttttttct ttttccgttg aagatgcctc
       61 aaacccgggg ccaaatactt ctttgatcat tgccaccagt attttatagt agtactggta
      121 atctgttact gtaccggaga aataagactg ggagaaacgg atcttcaaat actggtcaat
      181 attatcttct tcttcgtctt cttcgtaaag gattgttcca tcgcccattt caaatttcac
      241 attacgtaca tcggtccaca tttcatcttg ctctgtcagg ttttttttga tagcaggaag
      301 tcctttctta attcggatga atttctcttt tacacctttt tgtgaaccaa tgggaggatt
      361 gttttgggcc tgggcagtaa ttgtatacgc aaagaagcag gccaggatga tcagattttt
      421 catattcagt ttattactgt taataacaaa ttagaataaa aattcatgac ggcataaaac
      481 attacattct ttccggcact ttaatcccca gcaaactcat cgcagaagca atcacattcg
      541 cagtcatttc agcaatttgt aatctcaatt ctttcttctc ttcagtttct gcattcatca
      601 ctgaaagttc tgtataaaac gtattaaacg atttggccag gttaaaagca tatatcgcca
      661 gcacagaagg attgtgctcc gcaactgcct gctcaattat tacaggatat tgctccagcg
      721 aaacgatcag gtctttttcc agtttatgta acggacttgt agtttgtaac tgtttacctg
      781 cagtttgttt tctcaatata gatttgatcc ttgcatgggt atattgaaca aacggcccgg
      841 tgaaaccatg aaaatcgatg gactcttccg ggttaaagat cattttcttt ttcgggtcca
      901 ctctgagcag gaagaacttt atcgctccca gtgcaatcgt gtcatataat tccttcagct
      961 ctgcttcagt gaagtctttt actttgccta attcttctgt ttttttacgg gctacattca
     1021 ccatttcttc gatcatatca tccgcatcaa caacagtgcc ttcccggctt ttcattcggc
     1081 cggttggcag ttccaccata ccataactta aatgataaat tccatcggca tatggtttac
     1141 ctaatttctg caaaatgagt ttcagcactt tcatgtggta gttctgttca tccgctatca
     1201 cataaatgct ttggttgtag ttgaactctt catatttctg ctgagctaga ccaaggtcct
     1261 gggtgatata tacagatgtg ccatcttttc tcaatactaa tttttcatca agaccttcag
     1321 ccgttaaatc aatccaaacg ctgccatctt cttttttaaa gaatactccc tgcatcagtc
     1381 cttcttccac aaatttctta ccgggcaggt atgtttcact ttcataatac attttggcaa
     1441 aatcagaccc gatgcgttta taggtcacat caaaaccggc atatacccaa ccgttcattt
     1501 ttttccataa ttccatcacc gcagggttgc cggcttccca gtccaatagc atttgttgtg
     1561 ttgctttcat aatgggtgct tctttttctg cttcctcctt tttcattcca cccgcaataa
     1621 gttcttctgt ttgcctttta tattcatcat taaacttcac atagtattcc cctacaaagt
     1681 ggtcgccttt ggtatgggta gattgcggag tggctccgtc tgcaaatagc tgccaggcga
     1741 tcatgctttt acagatatga attccccggt cgttgacgat acaacttttt attacttcat
     1801 acccactttc cctcagcatc tgcgccacac tccagcctaa aaaattattt cgtaaatgac
     1861 ccaggtgcag cggtttatta gtgttgggag aggagtactc caccattact ttttgtccgt
     1921 tggctggctt aaaaccaaat tttgtattgg catagttaga ttgcaataca gttgtccaaa
     1981 aacgatcggt aactgtcagg ttgagaaaac ccttgatgac attatacccg ctaaataaac
     2041 caggatcgga ttgtataagg gcttcgccga tctcagtacc caattgctca ggtgattttt
     2101 tcaattgttt tacaaaagag aaaagaacca gtgtataatc tccttcaaac tccggtttgg
     2161 tgctgctaat agtgatggca gtctcattaa aggcctgtcc gtacaattct tccagggtct
     2221 ttattacaag gggctttatc tggcttacga tactcatcct gtcattttat ttgcagcaaa
     2281 tttacggttc ggatttggca tttggcggtt gtttcaatta ctttgccctt ccatgcgaag
     2341 acatatacat aatgccaggg atttcatcct tcccattatt gatttttttt atcctccctt
     2401 tcgccggttg atgaacctgc agaccttccg ttatgcagct tccgggggtg ggaatgtgat
     2461 gctggggttt attatctact ctttaagtta tacttacttg ttttttggcc gcgattttaa
     2521 ttttggtttt tatgccttca aagggcacag tgcggcctta tttgtttcct tttgtgtcac
     2581 tttcccgatt ggctttttta tgtcgaagta tgtagtgttc agcgattcta aaatgaaggg
     2641 aagaattcag ctcttccgtt actttatgat ctgcctgttc aacctggcaa tgaattatct
     2701 tttgctaaag atatttgttg agcggcttaa tgttcatccc atactgtcac aggtaattac
     2761 tgttgcaatt gttgttgtat tcagttacct ggcacagcgt aatttttctt tcaaggtgtc
     2821 tgccattgac gatgaactta aagactgaaa ctgccggaat ccacttttac ggacaggttg
     2881 ccctcacaat tactcctgat aaccaaatgc tgctgcatag ggtagatgcc gatcaattgg
     2941 atgtcgttta tattgccgta gctgctgata cccttcatcc aggactggtt taattgctgg
     3001 ttaaaacttg ttttggccgt atcgatataa gcgcccagtt cgaaacgggc ataccgggtt
     3061 atttctttcg taattttttt atcaaatagc cagtctgctg atcccagcaa tacatttttt
     3121 gatttgatat caaaatctat attcgttata gcaatggttc tgctggctga atcatacacc
     3181 ggctttccca ccaggtaaac aattccctgg ttggtgcctg aaaaatttat tttaatgatc
     3241 agtttttcaa aaccgccacc ataaatccgg caactgtcaa taacaaactt cttcctgaca
     3301 aatgcttttt taaaatcaaa gctcataccg gctacctgca tgttcaggat cgagctgagt
     3361 gaatcataat tcagcatggc atcaagaaaa atcgaaaatc ctttccggct acctgcattg
     3421 ttgatatgag gaacccagga gctttcttct gccggctttt caaaactaac gaccggtttg
     3481 gccgataaac ccaggaaaat attcagcgaa tcattctgcg catacagcct gttaatatga
     3541 actccttttg gatttatttt taaccagcca agtccgtata tatcatatac tttattcagc
     3601 tggtcccata ttttttgaaa ctgtggcttc aggtcaacgg tgccataatt tttatccagg
     3661 tctttctttg cagcatcaag ctctgctacc aggcccttca tcacctgttt ggttatattc
     3721 tgcccccaga agcatacttc acatttatca atgggctgtg gttcatttct gttgatggcc
     3781 agtttcactt tataattcgt catcagggtc aatgaattgg taaaggaaac atttaccctt
     3841 ctttccggct cattaaaacc acaacggcag ggtggtgtcc agggtgacag cactacacca
     3901 ttggcgcaga ccctggtgga tccgattatt ttataatacc cggtaaagcc caggttaagc
     3961 gacatacccg atgcctgcat ctgtaatttg cttcgcctga aaatatattt ataacgggta
     4021 tcacaggctt cctgtaccca tccttccggg taaccagggg aagtaaaaag ggtgtccaca
     4081 cttttttcag ccattgcata aaccggctta aggtctatct ggatcggtat attgatctca
     4141 gaattgggta atgaatccag tttgaaattt ccgggtgaca gatccgggtt ggccggattt
     4201 attttctggg aataagaaga gaaaacgccg agtaaacaga ttaaaacaag aattcctttt
     4261 cctttcattt atattcttta gatgtatgtc attccgggcc tgacccggaa tcttttttaa
     4321 agagacctta tagtaagcat tgaattatgc ttcttaatgt caggtcaaaa gtagataaag
     4381 aaagcaaaaa gaattgtgaa gatattactt acaggttccg gggatttttc cattttttgc
     4441 ccagtctttc tacggttcct tttactcttt ccttcctggt ttcttctctt ttcgccgtta
     4501 cgatccattc aatgtattct tttttgttgg taaaggaaag tgtatcaaaa taagctgctt
     4561 cctttttatt cttttttaat gcagcagcca ggtcgtcggg cagttttatt tctttggttt
     4621 taggattcac ccagttgaaa atttctctct ccggtttccc tttcgcttct ttatttttat
     4681 ccgcctctgt tttagcacgg aaaccaaata cactccatgt atcattgaat gagatcagac
     4741 tgatccaggt tagcttatcg ctttcggcca gcaggcagtc ccatccttta tctctcgtaa
     4801 gatcggtttg aatttttgaa ctgccttttg gataataaac ccatactgtt acatccggct
     4861 tgttatcctg agcctgccga aggaccagtt tcatcacttt tttcatctct ttttccaatt
     4921 gcgctttact cagcacaaac caatgcacct ggttatagtt tttaccgctg tctgataatt
     4981 ttactccttc gggtaaaccc aataatcctt ttttaaaatc agccggagca tttaatgtca
     5041 gcaggttgta ttggggtttg atcctgagtt tgtcagaaat agaagtagcc atagtaaaac
     5101 atttatagtg tacacattaa agttaattac cgatatagat aaaaggtgcc caataatagg
     5161 gcttccggta ctcttctccg aactttcctt ccagaaagta ttgttttact tgccggatgg
     5221 ctgctttaaa agttagaccg tctttgttaa ccagtttgta aacctcttgc atgaagaggc
     5281 tggtgctttt atccgccaca ctccatagtg atacaatgag agaattggct cctgctacca
     5341 ggaaagagga agataatccc tgcacccctt cgccttgcat cagcattcct ttccccgttt
     5401 gacag
//
LOCUS       DAORTI010000186         5397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18399_length_5397_cov_6.611382, whole genome shotgun sequence.
ACCESSION   DAORTI010000186 DAORTI010000000
VERSION     DAORTI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18399_length_5397_cov_6.611382"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..443
                     /locus_tag="PLO70_15470"
     CDS             126..443
                     /locus_tag="PLO70_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ75919.1"
                     /translation="MQHLKDLQPKELAPGLTGYYAHGTNMTLGLVEIKAGSNLPLHQH
                     LHEQITYILEGQLDMTIGGQPCSLTAGMYYVIPSNVPHGAVAVTDCKVIDVFNPVRED
                     YKL"
     gene            complement(454..1005)
                     /locus_tag="PLO70_15475"
     CDS             complement(454..1005)
                     /locus_tag="PLO70_15475"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ75920.1"
                     /translation="MVVTGFNPFPELSTERLLLRQLRFSDAHCLAELRSNKKVNEHID
                     RPATMQTEEANNFIEKVNRRMKDGELLYWVIALKGTGSFAGAICLWNIEADKDRAELG
                     YELHPDYQGRGIMQEALHTVIEFGFAKMKLAVILALTKPANKASEKLLIKNSFIPDED
                     YRYVSKENANEYLVYYLLSGSRV"
     gene            complement(1043..3301)
                     /locus_tag="PLO70_15480"
     CDS             complement(1043..3301)
                     /locus_tag="PLO70_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQZ75921.1"
                     /translation="MELKYSQQIAGTLNLSLKQISSIYELHSEGSTIPFIARYRKEAT
                     GNLDEVVIGNVIDQIKYFNELEKRKETVLKTIEEAGKLTPELKKRIEDCINATELEDI
                     YLPYKPKRKTKASVAIEKGLEPLAKLLFEQGPENPEAAAVKFINEQVKDTAEALQGAR
                     DIMAEWVAENEQARNIVRKLFTEEATVSSRVLTTKKDEADAQKYRDYFEFKEPLAQSP
                     SHRILAIRRGEKEGFLIMTINIEKEMAHAELKRVFLKSPGLAAKQVEHAVEDSYNRLL
                     QPSIETEFRMASKTKADEEAIRVFAENLRQLLLASPLGQKRVMAIDPGFRTGCKVVCL
                     DEHGTFLKYNAIFPHAPQNDWNGATQTIKELVAKYDIEAIGYGNGTASKETELLVRAI
                     DFGKPVSVFMVNESGASIYSASEVAREEFPDQDVTVRGAISIGRRLLDPLSELVKIDA
                     KSIGVGQYQHDVNQNRLKDALDQVVESCVNFVGVDVNTASKHLLMYVSGLSATVAKNV
                     VEYRTKNGGFRSRDELKKVSMLGPKAYEQCAGFLRIVNAANPLDNSAVHPESYHVVEA
                     MAAKANSTVPELIKNPEIRKQLKAKDFVSETIGQFTIEDILKELEKPGRDPRSPIEEF
                     RFAEGVSSMEDLKPGMKVPGIITNITNFGAFVDVGVKQDGLVHISHLANRYISDPNEA
                     VKLGQNVMATVLEVDIARKRIALSLKDGAERTEVRSTKSEVRGQKPVVKKTEPMNPFQ
                     AKLMELKKNFKD"
     gene            complement(3435..4571)
                     /locus_tag="PLO70_15485"
     CDS             complement(3435..4571)
                     /locus_tag="PLO70_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ75922.1"
                     /translation="MSKIHLYIAFIILLASCSSKKQAEAGTKETIAEDMVTLTDAQLK
                     NAGIETGTVQEQTMNSSLKVNGMVDVPPQNLVSVSFPLGGYLRSTKLMPGMHVNRGEV
                     IAVIEDQALIQLQQDYLVAVAKLGFLQKEFERQKMLNENKVNADKVYQQAEADYNSQK
                     VFVKGFSEKLHLIGISPARLTESNISRSVTVHSPINGFVSKVNVNIGKYVTATDVLFE
                     LINPDDMHAMLTVFEKDITKVKIGQKVQVSFVDDPGAVYECEVILVTKNVDESRSALV
                     HCHFEKQPKNLLPGMFLNATISISNAKVLTLPEDAIVRFENKQYVYEITGKNEFRMLE
                     VATGMVENGRVEVSSSTEGLAGKTLVVKNAYAILSKMKNTAEEE"
     gene            complement(4577..>5397)
                     /locus_tag="PLO70_15490"
     CDS             complement(4577..>5397)
                     /locus_tag="PLO70_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQZ75923.1"
                     /translation="LLRLDSVYSRVLQAAELRLKAGETNMLEKVTVEAQVQQLKIQQQ
                     QLQSDIGIAQQKLQWLLNTDQKLLPDYAESNAPGIINNDTSVIVHHPLLLYKEQQVAV
                     AGSLTALERNKLVPDVSVGYNNMSIVGYQSQDGVTQQYYGAGSRFNSFNLTLAVPLFS
                     KAAKNKVKAGQLNEEIARMNVKVTDQQLKSQLLQLTEELKKRQQQLNYYNQQGLAQSE
                     LILSHAKQNYEKGQISYLEWTMLMNNAVDIQLAHLAAWQQLNIIRTEIEYLTGK"
BASE COUNT         1410 a         1286 c          954 g         1747 t
ORIGIN      
        1 gtaaaggaaa gatgattggt gtccggtata ataaagacaa agaatgggtt ggtggtagtt
       61 tttgcttgtt tgcctcctaa ccctgaagag aaatttgttt tcgaaaactg aactttataa
      121 tttttatgca acatttaaaa gacttacaac caaaagagct ggcacccggc ctcaccggct
      181 attatgccca tggaactaat atgactcttg gtttagtaga aataaaagca ggcagcaatt
      241 tacccctgca tcaacatctg catgagcaga tcacttatat actggaaggg caattagata
      301 tgaccattgg cggacagccc tgttctctta ctgccggtat gtattatgtg ataccttcca
      361 atgtgccgca tggtgctgtg gcggttactg attgcaaggt gattgatgta tttaacccgg
      421 tgagggaaga ctacaaacta taaactataa actttaaacc cttgaaccag aaagaagata
      481 atagaccagg tattcattcg cattttcttt gcttacatat ctgtaatctt cgtcaggaat
      541 aaaggagttt ttgatcaata acttttcaga agccttattt gctggtttag taagcgcaag
      601 aataaccgcc agtttcattt tggcaaaacc gaattcaatc actgtatgta atgcttcctg
      661 catgatgccc ctgccctggt aatcaggatg caactcgtaa cctaactcgg cccggtcttt
      721 atctgcttca atattccaga gacaaatggc gcctgcaaaa ctcccggtac cttttaaagc
      781 aatgacccaa tagagcaatt ccccatcctt catgcgccgg ttaaccttct caatgaaatt
      841 attagcctct tcggtttgca tggttgccgg acgatcgatg tgttcattta cttttttgtt
      901 tgaacgcagt tccgctaggc aatgagcatc tgaaaaacga agttgcctca gcaataatcg
      961 ttctgttgaa agttcaggaa aagggttgaa tccggtgaca accatttgca gcttctgttt
     1021 ctataaatat agctgcaatc ggtcaatctt tgaagttctt cttcagttcc atcagctttg
     1081 cctggaatgg attcatcggc tcggtttttt ttacaacagg tttctgtccc cttacttctg
     1141 acttcgtact tcgtacttca gttctctctg ctccgtcctt caacgacaat gcaattctct
     1201 ttcttgcaat atccacttcc agtacggtgg ccattacatt ttgtcccagt ttcacggctt
     1261 cattgggatc actgatataa cggtttgcta aatgagaaat atgcaccagc ccatcctgct
     1321 ttacccccac atccacaaag gcaccaaaat tggtgatgtt ggtaatgatg cccggtactt
     1381 tcatacccgg cttcaggtct tccatactgc tcaccccttc tgcaaaacga aattcttcaa
     1441 taggagaacg ggggtcacgg cccggcttct ccagttcttt caatatatct tcaatggtga
     1501 actggccgat cgtttcagaa acgaaatcct ttgcttttag ctgtttcctg atttccggat
     1561 tttttatcaa ctccggaaca gtactgttag ctttagcagc cattgcttcc accacatgat
     1621 aactttcagg atgcacagca gaattatcca gaggattagc ggcgtttacg atacgtaaga
     1681 acccagcaca ttgttcatat gctttcggac caagcatgga gactttttta agttcatcac
     1741 gacttctaaa accgccattc tttgtcctgt attccaccac attttttgct accgtcgcac
     1801 ttaacccgga aacatacatc agcaggtgtt tactggcggt attcacatcc acaccaacaa
     1861 agttcacaca gctttccact acctggtcaa gggcatcttt caagcggttc tggttcacat
     1921 catgctggta ttgacccacc ccaatactct tggcatcgat cttcactaac tcacttaagg
     1981 gatctaataa tcttctgccg atacttatcg ctccgcgaac ggttacatcc tgatccggaa
     2041 attcttctct tgccacttca ctggcagagt aaatagaagc gccgctttca tttaccataa
     2101 aaacagaaac aggtttacca aaatctatag ctctgaccaa cagctcagtt tctttactgg
     2161 ccgttccatt accataaccg atggcttcaa tatcatattt tgccaccagt tcttttattg
     2221 tttgcgtggc accattccag tcattttgcg gtgcatgtgg aaatatcgca ttgtatttta
     2281 agaaagtacc atgttcatcc aggcaaacca ctttacaccc tgtcctgaaa cccggatcga
     2341 ttgccatcac tctcttttgt cctagtggtg acgccagcag caattgacga aggttttctg
     2401 caaacaccct gatcgcttct tcatccgctt tcgttttact ggccatcctg aattctgttt
     2461 caatagaagg ctggagtaaa cgattgtatg aatcctcaac agcatgttca acttgctttg
     2521 ctgcaagacc aggtgacttc aggaaaaccc gtttgagttc tgcatgggcc atttcctttt
     2581 cgatattgat ggtcataatg agaaaacctt ccttttcgcc tctgcgaatt gccagtatac
     2641 ggtgtgacgg actttgcgcc aatggttctt tgaattcaaa ataatccctg tatttctgcg
     2701 catctgcttc gtccttcttt gttgttagta ccctggagga aacagtagct tcctcagtaa
     2761 ataatttacg gacaatattc ctggcctgtt cattctctgc gacccattct gccattatat
     2821 ccctggcacc ttgtaaagct tctgccgtat ctttcacctg ctcattgata aatttaactg
     2881 ctgctgcttc cgggttttca ggtccctgct caaataataa ttttgccagt ggctccaatc
     2941 ctttctcaat cgctacagaa gcttttgttt tccgtttagg cttatacggc agataaatat
     3001 cttccagttc agtagcattg atacaatctt caattcgctt tttcagttca ggtgtcaatt
     3061 tacctgcttc ttcaatcgtt ttaagaactg tttcttttcg tttttccagt tcattgaaat
     3121 atttgatctg gtctattaca tttccaatca cgacttcatc caggttcccg gtagcctctt
     3181 ttctgtaccg ggcaataaaa ggaatagtag aaccttcact gtgcagttca taaatactgc
     3241 tgatctgttt tagtgaaaga ttaagggtac cggctatctg ttgtgaatat ttcagttcca
     3301 taggtctttt gtttggggga gcaaatctaa tggtagcagc agaaaaaaaa atttgttttt
     3361 acccacaaat tccgatagta aagtagctga cccgtttacc agtcagctac tttaaaaatc
     3421 aatagcagag tacactactc ttcttcagcc gtatttttca ttttggacaa aatagcataa
     3481 gcatttttaa ctaccagtgt tttacctgca agtccttctg tactgctact cacttccacc
     3541 cttccgtttt ccaccatacc agtggctact tccagcatcc tgaattcatt cttacctgtt
     3601 atttcataaa catattgctt attctcaaaa cgaacaattg catcttcagg cagtgtaagc
     3661 acttttgcat tacttatact gatcgttgca ttcagaaaca ttcctggcaa taaatttttt
     3721 ggctgcttct caaaatgaca atgcaccaaa gcactacgac tctcatctac atttttagta
     3781 accagtatta cttcacactc atatacagca ccgggatcgt ccacaaaaga gacctggact
     3841 ttctgcccga tcttaacttt ggtgatatct ttttcaaaaa ccgtcagcat ggcgtgcata
     3901 tcatcaggat tgatgagttc aaaaagtacg tcagtagcag taacatattt gccaatattt
     3961 acattcactt tagaaacaaa gccattaatt ggtgaatgca cagtgacact tcttgatata
     4021 ttactttcag taagacgggc agggctgata ccgatcagat gtaatttttc actgaaccct
     4081 ttaacaaaca ctttttgcga attataatca gcctctgcct gctggtacac tttatccgca
     4141 ttcactttat tttcattgag cattttttgt ctctcaaact ccttttgcaa aaaaccaagc
     4201 tttgctactg ccactaaata atcctgctgc aactggatca aggcctggtc ttcgataaca
     4261 gcgatcactt cgccccggtt cacatgcatc cccggcatca gtttagtgga ccgcaaataa
     4321 ccacctaaag gaaaactcac tgaaactaga ttctgcggcg gcacatccac cattccattc
     4381 acctttaaag aactattcat agtttgttcc tgtacagtcc cggtttcaat accggcattt
     4441 ttaagttgtg catccgtcag ggtcaccata tcttcagcta tggtctcctt tgtacctgct
     4501 tctgcctgtt tttttgatga acaggaagcc aacagaataa taaaagcaat atataaatga
     4561 atctttgaca taatttttat tttccggtta aatattctat ttctgttcga attatattca
     4621 gctgttgcca tgctgccagg tgagccaatt gtatgtcaac cgcattattc atcagcatgg
     4681 tccactcaag gtaactgatc tgccctttct cataattttg ttttgcatga ctgaggatca
     4741 gttctgattg agctaacccc tgctgattat aataattcaa ttgttgctga cgttttttca
     4801 gttcttctgt tagctgcaat aattgacttt tcaactgctg gtctgtcact tttacattca
     4861 tccttgctat ttcttcattc agctgcccgg cttttacttt attctttgcc gctttactga
     4921 acaaaggaac agccagtgtc aggttaaaac tattgaacct gcttccggca ccataatatt
     4981 gctgtgtaac accatcttga gattggtaac caactatcga catattatta tagcctacag
     5041 acacatcagg caccagttta tttctttcca atgctgtcag cgaacctgcc acggctacct
     5101 gctgctcttt ataaagcaac aatggatgat ggacaattac tgatgtatca ttattaataa
     5161 ttcccggtgc attactctcc gcatagtcag gcaatagttt ttgatctgta ttcagcaacc
     5221 attgtaactt ttgttgtgca atgccgatat cgctctgtaa ttgctgttgc tgaattttta
     5281 attgctgcac ctgtgcctcc acagttactt tctccagcat atttgtttca cctgctttca
     5341 accggagttc tgcagcctgt aacacacggc tgtacacaga atccaaccgc aatagca
//
LOCUS       DAORTI010000187         5362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18597_length_5362_cov_6.478990, whole genome shotgun sequence.
ACCESSION   DAORTI010000187 DAORTI010000000
VERSION     DAORTI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18597_length_5362_cov_6.478990"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLO70_15495"
     CDS             <1..880
                     /locus_tag="PLO70_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQZ75924.1"
                     /translation="VIYIINEKGELIDDIRIRDIILSPPDKKVTELIDERFIALHPED
                     DQETASEVFKMNNRVALPVVSKSNKLLGIVTIDDILWVASEEFSEDMQKMGGTAALEE
                     PYLDVPLFKLYKKRIIWLIILFVGETLTIAAMSGFQKTLEQVLVLSTFIPLIISSGGN
                     SGSQAATLIIQAMALGEISVKDWWQIARREIQSGLFMGITLGAIGFLIVFGGHHIFDL
                     FGEHFIRIAFAIGIAIIGVVLWGTIMGSMLPLLLKRLGADPATSSTPFIATLVDVTGL
                     LIYFGTAFLLLRGVLL"
     gene            1002..2840
                     /gene="glmS"
                     /locus_tag="PLO70_15500"
     CDS             1002..2840
                     /gene="glmS"
                     /locus_tag="PLO70_15500"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQZ75925.1"
                     /translation="MCGIVGYTGPREAYPIIIKGLKRLEYRGYDSTGVALQNGSGLKV
                     YKKKGKVAELEDSIVGKNLHANAGIGHTRWATHGEPSDRNAHPHQSASGKLAMIHNGI
                     IENYNQLKKELSNKGYTFSSDTDTEVLLNFIEEIKKNNNCSLEEAVRVALKRVTGAYV
                     ILLLDADDPETIIAARKGSPLVIGVGKGEHFLGSDASPMLEYTKEVVYVNDYELAIVK
                     PDELILKNLGNEKITPYVQKLDIELAAIEKGGYDHFMLKEIFEQPQTIYDCLRGRLDA
                     TEGTITMSGIQQYAEQIMNANRIVMVACGTSWHAGLVAEYVFEELCRINVEVEYASEF
                     RYRNPVINKGDVIIAISQSGETADTLVAIENAKSKGAIILGVVNVVGSSIARMSHAGA
                     YTHAGPEIGVASTKAFTAQLAVLTMIALKIGYIKGTISQERYMHLLKELQEIPEKAAW
                     TLKHSDNIRALAEKYKDARDFLYLGRGFNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALVDENLPVVFVATKDSFHEKVVSNIQEIKARKGKVISVITEGDETSASLSEDVM
                     IIPEADEIMAPMLSVIPLQLLAYYIGLAKGCDVDKPRNLAKSVTVE"
     gene            2858..5065
                     /locus_tag="PLO70_15505"
     CDS             2858..5065
                     /locus_tag="PLO70_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HQZ75926.1"
                     /translation="MNHINKKAISSLGYGFIPKEFIPKGKNEYHLRNIQNRSGIRYRK
                     LTAAEIKVLEKNDNSSDNWSKVLVSDSFDPKLVKRCSFFGLVRIGKLEPFFHEFNNLR
                     MPVGLYNSTIISSDIGDNVVIDNVNFMSHYIIGNDTMLVNINELATTDHSKFGNGVLK
                     DGENEAVRVWMEICNENAGRSVLPFNGMLAGDAWLWSKYRDDEKLLQKFKEFTDKKFD
                     TQRGYYGKIGDRTVIKNCRIIKDVWIGSDAYIKGANKLKNLTINSAPEAKSQIGEGSE
                     LVNGIISFGCRIFYGVKAVRFMMGSNSQLKYGARLINSYLGDNSTISCCEVLNSLIFP
                     AHEQHHNNSFLCAATVFGQSNMAAGATIGSNHNSRGADGEIIAGRGFWPGLCVSLKHN
                     SKFASFTIIAKGDFPSELNIPVPFSLVSNDISNDKLVIMPGYWFMYNMYALARNAGKY
                     TSRDKRSDKTQEIEYDFLAPDSINEMFESLELLKKFTAQAYARKTNKQIPEKDLSKKG
                     EALLERNKEEIEELEILAEGFENSSRKVQLIKVPEAYGLFKELITYYGISQLLRFIKS
                     HNIISWEKLVARLPRNPDRNKWKNIGGQLIPDTAISSLTRSIRFGKMTGWDEVHEFYN
                     KKSKSYQTDKFNHAFASLLEILKLSPGKFTKKTFRKLLHQAVTTREWMTKGIYESRAK
                     DYQNPFRSMVYDTQEEMDKVIGKLKDNKFILLQQEETIGFKKQIEEIIARFEL"
     gene            complement(5169..>5362)
                     /locus_tag="PLO70_15510"
     CDS             complement(5169..>5362)
                     /locus_tag="PLO70_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75927.1"
                     /translation="AIIAITGFMVACGDNKEANKADEVTEEVKEAVENKVEEVKDTAV
                     KMVENAMDTVAKKVEEVKK"
BASE COUNT         1751 a         1001 c         1126 g         1484 t
ORIGIN      
        1 tgtcatctat attatcaatg aaaaaggtga attgattgat gatatacgca tccgtgatat
       61 tatactaagt ccgcctgata agaaagtgac tgaattaatt gacgaaagat ttattgccct
      121 ccatccggaa gacgaccagg aaaccgccag tgaagttttt aaaatgaata accgggtagc
      181 ccttccggtg gttagtaaaa gcaataaatt actgggaatt gttaccattg atgatattct
      241 ctgggtagcc agcgaagaat tcagtgaaga catgcaaaaa atgggaggca ctgctgcttt
      301 ggaggagcct tatcttgatg tcccgctttt taagctttat aagaaaagga taatctggct
      361 gatcatcctt tttgtaggcg aaacgcttac cattgctgcc atgtccggct ttcaaaaaac
      421 actggagcaa gtcctggttc tatcaacgtt tatcccatta atcatttcca gcggaggtaa
      481 cagcggttca caagctgcca ctctgattat acaggctatg gcactgggag agatctctgt
      541 aaaagattgg tggcaaattg cccgccggga aatccaatca ggcctcttca tgggaataac
      601 acttggcgcc ataggttttc tgatagtatt tggaggtcat catatttttg acctttttgg
      661 agaacatttt attaggatag cttttgcgat tggaattgct attatcggcg tagtactatg
      721 gggtacaatc atgggttcaa tgctgccgct cttactgaaa agattaggcg ctgacccagc
      781 aacttcttct accccattta tagccactct tgtggatgta accggactgc tcatctattt
      841 tggcacagcc ttccttttac tccggggcgt tctgctttga gaatcaaaaa acgggtatag
      901 accttgtatt cataaatatt cattttgttt tattttttgc aaccggtcaa acgtttgcat
      961 gttttgaaat tgataccttt gctgcactaa atcaaaaacc aatgtgtgga atagtcggat
     1021 atacaggtcc aagagaagct tatcctatca tcattaaagg gctaaaacgc cttgaatacc
     1081 gtggctatga tagtaccggc gtagccctgc aaaacggcag tggccttaaa gtatacaaga
     1141 aaaagggtaa agtggcagaa cttgaagaca gtattgttgg caagaaccta catgccaatg
     1201 ctggtattgg acatacccgg tgggccactc acggcgaacc cagcgaccgg aatgcacatc
     1261 cccaccaatc tgccagtggc aaactggcca tgatacataa tggcattatt gaaaattata
     1321 accagctgaa aaaggaactt tctaataagg gctatacttt cagcagtgat acagatacag
     1381 aagtcttgct gaattttatt gaagaaataa agaaaaacaa taactgctca ctggaagaag
     1441 ctgtcagggt ggcattaaaa agagtaaccg gagcttacgt aatcctttta ctggatgccg
     1501 atgaccccga aactatcatt gcagccagga aaggaagtcc tttagttatc ggagtgggta
     1561 aaggagaaca ttttctgggc tctgatgctt cccctatgct ggagtataca aaagaagtag
     1621 tctatgttaa cgactatgaa ctggctattg taaaaccaga tgagcttatc cttaaaaacc
     1681 ttggcaatga aaaaataact ccttatgttc aaaaactgga tatagaactg gcagcgattg
     1741 aaaaaggtgg ttacgatcat tttatgttaa aagaaatttt tgaacagccg caaaccattt
     1801 atgactgtct tcggggaagg ttagatgcaa cagaaggtac tatcaccatg agtggtattc
     1861 agcaatacgc agaacagatc atgaatgcaa atcgcattgt catggtagct tgtggcacca
     1921 gctggcatgc aggtttagtg gctgaatatg tttttgaaga attatgccgc ataaatgttg
     1981 aggttgaata cgcatctgag ttccgataca ggaatccggt tatcaataag ggagacgtca
     2041 tcatcgctat ctcacaaagt ggagaaactg cggatacttt agtagcaatt gaaaatgcaa
     2101 aatcaaaagg tgcgattatt ttaggagtgg tcaacgtagt tggctcatca attgcaagaa
     2161 tgagccatgc aggtgcttac acccatgcag gaccggaaat aggtgtggca tcaacaaaag
     2221 cattcaccgc acagcttgcc gtactcacta tgattgcctt aaagatcggc tatataaagg
     2281 gtaccatttc acaggaacgt tacatgcatc tgctgaaaga attacaggag attccggaga
     2341 aagcagcctg gacactaaaa cacagcgata atatcagagc tttagctgag aaatataagg
     2401 acgccaggga ttttctttac ctgggtcgtg gctttaattt ccccgttgcc ctggaaggcg
     2461 ctttaaaatt aaaagagata tcctacatac atgcagaagg ataccctgct gccgaaatga
     2521 aacacggacc tattgcgctg gttgatgaaa atcttccggt ggtgtttgta gctacaaaag
     2581 attccttcca tgaaaaagta gttagcaaca tacaggaaat taaggccaga aaaggtaaag
     2641 tgatatccgt aattacggaa ggggatgaaa catcagcttc tttatcagag gatgtgatga
     2701 tcattccgga agcagacgaa ataatggcac ctatgctgag tgttatcccc ctgcaattac
     2761 tggcttatta tatcggatta gccaaaggat gtgatgttga caaaccaaga aaccttgcca
     2821 aatctgtaac cgttgaataa agcagtacca atcaagcatg aatcacataa ataaaaaagc
     2881 aatttcttct cttggatatg gctttattcc aaaggaattt attcctaaag gaaaaaacga
     2941 gtaccacctt cgcaacatac agaacaggag tggaatcagg taccggaaat taaccgctgc
     3001 agagataaaa gtgcttgaaa aaaatgataa cagctccgat aactggtcta aagtacttgt
     3061 atctgattcc tttgatccta aacttgttaa acgatgttcc tttttcggat tagtcaggat
     3121 tggcaagctg gaaccctttt ttcatgaatt caataatctg cggatgccgg tgggtttata
     3181 taacagcact attatcagca gtgatattgg cgacaatgtt gttattgata atgtaaattt
     3241 tatgagccat tatattatcg gcaatgacac catgctggta aacataaatg aattagcgac
     3301 aacagaccat tctaaattcg gtaatggtgt tttaaaggac ggtgaaaatg aagctgtccg
     3361 tgtatggatg gaaatttgta atgagaatgc cgggagaagt gtactccctt ttaatggtat
     3421 gctggcgggt gatgcgtggc tctggtcaaa atacagggat gatgaaaaat tattgcagaa
     3481 attcaaagaa tttaccgata aaaaatttga tacccaaaga ggttactatg gaaagattgg
     3541 cgaccggaca gtaataaaaa actgccggat tattaaagat gtttggatag gaagtgatgc
     3601 ctacattaaa ggtgcaaata aattaaaaaa ccttaccatc aattctgctc ccgaagcaaa
     3661 atcgcaaatt ggtgaaggaa gtgaactggt aaatggtatt attagttttg gttgcaggat
     3721 attttatgga gtaaaagctg ttcgctttat gatgggctcc aactcgcaat tgaaatatgg
     3781 tgcacggtta atcaattcat acctgggaga taattcaacc atttcctgtt gtgaagtatt
     3841 gaattcattg atttttcctg cccatgaaca acaccataat aactcgtttt tgtgtgcagc
     3901 aacagtattt ggccaaagca atatggctgc cggcgccaca attggttcca atcataattc
     3961 aaggggtgcc gacggtgaaa taattgccgg ccgtggattc tggcccgggc tatgtgtaag
     4021 tctaaaacat aattcaaaat ttgcctcttt cacgattata gccaaaggcg acttcccatc
     4081 tgaattaaat atacctgttc ccttttcttt ggtaagtaat gatatcagca atgataaact
     4141 ggtaataatg cccggttatt ggttcatgta taatatgtat gcattggcca gaaatgcagg
     4201 caaatacacc agtcgtgaca aaagatcaga caagacacag gaaatagaat acgatttcct
     4261 cgctcctgac agtataaatg agatgtttga atcgctggaa ctgctcaaaa aatttactgc
     4321 acaggcatat gccagaaaaa caaataaaca aataccggaa aaagacttat caaaaaaagg
     4381 cgaggcatta ctggaaagaa acaaagagga aattgaagaa ttagaaatac tggcagaagg
     4441 ttttgagaac agcagccgga aagttcaatt gataaaagta cctgaagcct atgggctttt
     4501 taaagagctt ataacttatt atggaatcag ccagttactc cgctttatca aatcgcataa
     4561 tatcataagt tgggaaaaat tagttgccag gttaccacga aatccagatc gtaataaatg
     4621 gaaaaatata ggaggacaac taataccgga cacagctatc agttctctta caagatcaat
     4681 tcgctttgga aaaatgaccg gctgggatga ggtacatgag ttctataata aaaaaagtaa
     4741 gtcatatcaa actgataaat tcaaccatgc gtttgcctct ttattggaaa tacttaagct
     4801 aagccccggt aaattcacca aaaagacgtt tagaaagctt ttacaccagg ctgttactac
     4861 cagggaatgg atgacaaaag gcatatatga atcaagagcc aaagattatc agaatccatt
     4921 ccgttcgatg gtgtatgata cacaggaaga aatggacaaa gtaattggca aactaaaaga
     4981 caataaattc atccttctgc agcaggaaga aacaatcggc tttaagaaac aaatcgaaga
     5041 gattattgcc cgttttgagc tctaaaaatt ctatcatatt ttttttaatt acaagatctt
     5101 tctcaaaaaa atacccccgg acaatccggg ggtatttttt aagaagtaat acagtgaata
     5161 tataataatc actttttaac ctcttcaacc tttttagcaa ctgtatccat agcattttca
     5221 accattttaa cagcagtatc ttttacttct tccactttat tttcaacagc ctctttaact
     5281 tcctcagtta cttcatctgc tttatttgct tctttgttgt ctccacaggc caccatgaaa
     5341 cctgtgatag cgataattgc ga
//
LOCUS       DAORTI010000188         5341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18727_length_5341_cov_9.530079, whole genome shotgun sequence.
ACCESSION   DAORTI010000188 DAORTI010000000
VERSION     DAORTI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18727_length_5341_cov_9.530079"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLO70_15515"
     CDS             complement(<1..920)
                     /locus_tag="PLO70_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQZ75928.1"
                     /translation="MKIAGSEIKTIFLRVVFFYLLPVTFFIGCGSKDNPPPPPPVRNP
                     VFIAATVNNSSLENTVYDVSLQPVIKVNFSEPLQPGTVAAAVSVADINGVSVAVSVTV
                     ENSDKSIVIQPTATLGHLKKYFIRVATSLLSSSGGQLQSQVSKNFITKIDSSRKFPVI
                     SDNALLDLVQQQTFKYFWDFAHPVSGLARERNTSGDIVTSGGSGFGIMTIPVGINRNF
                     ITRAQGLARMQKIVAFLKNTAVKVKGAFPHWLDGNTGAIVPFSTNDNGADLVETSYLV
                     MGLLTARQYFNGVDAAETTLRNDINTLYNNV"
     gene            complement(925..2391)
                     /locus_tag="PLO70_15520"
     CDS             complement(925..2391)
                     /locus_tag="PLO70_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HQZ75929.1"
                     /translation="MKKLFIVITALGLFSCSDNKEKPVTTSSAASFSTDGKKVIVYTT
                     ADSTAYRLSVTDTLVFKEMGQPKETEICVFVDPSKTFQTYFGTGAALTDASAETFYKL
                     PKDKQQEVLQAFFDKEKGIGYTVARTNINSCDFSSDMYTYVADNDTALKSFNIDHDRK
                     YKIPFIKEAMKAAGGKLDLFASPWSPPAWMKDNNNMLQGGKLKPEFYNSWAMYYTKFI
                     KEYEKEGVPVWGISVQNEPMATQRWESCLYTAEEERDFLKNHLGPTMEKEGLKDKKII
                     VWDHNRDLIYQRAQTYFNDPEAAKYAWGIGFHWYEDWSGGTPMYENLKRVYEAFPDKN
                     IIFTEGCAESFNASRYNAWVLGEEYGRSMINDYNNGMVGFTDWNIILDETGGPNHVQN
                     FCFAPVHGDTKTGQLIYTNAYYYIGHFSRFIQPGAKRISAAASRSQLLTTAFRNEDGK
                     TVVIVMNQGDLSFPYYLWINGKAAEVKARPHSIATLIF"
     gene            complement(2415..3845)
                     /locus_tag="PLO70_15525"
     CDS             complement(2415..3845)
                     /locus_tag="PLO70_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQZ75930.1"
                     /translation="MQTKFLLPAFLLAGILSLSSNCSKNNAGGGTQSPSPAIEYWITK
                     GDRSVLLQKQPTALNFTTTANSFTNIEVDSTTKFQEIDGFGYTLTGGSAYLINRMDAP
                     SRSSLLNELFGSGNDAIGISYLRISIGASDLSPSVFSYNDMPAGQTDPTLANFSLSLD
                     TVDLVPVLKQILTINPQIKIMGSPWSPPVWMKDNGSSVGGSLLPQYYAAYAQYFVKYI
                     QAMAARGIKIDAITVQNEPQHGGNNPSMVMSSLQQAEFIKNHLGPAFQAATIKTKIII
                     WDHNCDNPNYPITILNDAAAKPFIDGSAFHLYAGEIGVLSGVKAAHPDKNLYFTEQWT
                     GANGSFDGDLKWHLKNVVIGSMRNWSKTALEWNLANDPSYGPHTPGGCTECKGALTIN
                     ASVVTRNVGYYIIAHASKFIPAGSVRIASTTGVSLHSVAFVRPDGKKVLIVLNDSGSS
                     TSFNIKYKGKWAATELPGATVGTYIW"
     gene            complement(3875..>5341)
                     /locus_tag="PLO70_15530"
     CDS             complement(3875..>5341)
                     /locus_tag="PLO70_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQZ75931.1"
                     /translation="KQQPSLLTDVSQNIVCNWKHKENEFVDFNVQYLIPHALSTRGPK
                     IAVADVNGDGLDDFYACGAKYQPGTLMIQQGNGSFIAADTAVFGPDAICEDVDAVFFD
                     ANGDGSPDLYVVSGGNEPRANALSQEDRLYLNDGKGKFSKSAAAFTQRYENKSCVTVA
                     DIDKDGDTDIFVGNLASSTAYGIPQTSYLMLNDGKGKFSYAGDNIIPLQSVGMVTAAS
                     FTDVNKDGWMDLVVTGEWMPLKIFINNKGVFSVTEIPQSTGLWQTVYTTDVNGDGFAD
                     ILAGNWGHNSKLFAGKNGPLKLYVKDFDLNGSTEQIMCYTVNGKEYTFLAKDELERPL
                     PVLKKAYLTYSEVAGKTVDFMFFDLFKDYLELKAETLSSSCFINDGKGNFKKVELPAE
                     LQLAPVMAFAPAGSGQAGYIAAGNFFGVIPYEGRYDALLPTFFSFDRTVNAFNYQTAI
                     SSFFGEARDIKWLNTAGNSKLMLIARNNKPLLFLKQTP"
BASE COUNT         1533 a         1208 c         1004 g         1596 t
ORIGIN      
        1 acattattgt ataacgtatt gatatcattt cgtaaagttg tttccgctgc atccactcca
       61 ttgaaatatt gccgggcggt tagcaacccc atcaccaggt atgatgtttc taccaaatca
      121 gccccgttat catttgtact gaacggaacg atggccccgg tatttccgtc gagccaatgg
      181 ggaaaagctc cttttacttt aactgctgta ttctttaaaa aagcaacaat cttctgcatc
      241 cgtgcaagac cctgggccct cgtaataaaa ttacgattga ttcccaccgg tatcgtcata
      301 ataccaaacc ctgagccacc ggatgtaaca atatcaccgg atgtatttct ttctcttgcc
      361 aaaccactta ccgggtgagc aaaatcccag aagtatttaa aagtttgttg ttgcaccagg
      421 tctagcaatg cattatccga aataacaggg aactttcttg aagaatcaat ctttgttata
      481 aaatttttac tcacctgcga ttgtaactga cctcctgatg atgacagtaa ggaagtagcc
      541 actcttataa agtatttttt cagatgaccc agcgttgctg ttggctggat cactatagac
      601 ttatcgctat tctctactgt tacagacacc gcgacggaga ctccgttgat atctgctacc
      661 gaaacagcag cggcaacagt gccaggttgc agcggttctg aaaaatttac tttgattact
      721 ggttgcaggc tcacatcata aaccgtgttc tccaatgagc tattgttgac cgttgcagca
      781 atgaaaacag ggttccttac cggtggtggc ggaggcgggt tgtctttact cccgcaacca
      841 ataaaaaaag ttactggtaa taaataaaaa aagacaaccc ggagaaaaat agtttttatt
      901 tcagaccctg ctattttcat aagatcagaa tattaatgta gcgatggaat gcggcctggc
      961 ttttacttct gcagctttac cattgatcca caaataatag gggaatgaga gatcaccctg
     1021 gttcatcact atcactaccg ttttaccatc ctcgttacgg aaagcagtag tcagtaactg
     1081 gctgcggctg gcagcagcac tgatacgttt agccccgggc tgaatgaacc tggaaaaatg
     1141 acctatataa taataggcat tagtatagat caactggccg gtctttgtat caccatgtac
     1201 gggagcaaaa caaaaattct gaacatggtt aggccctcct gtttcatcca gaataatatt
     1261 ccaatctgta aagcctacca tgccgttatt ataatcattg atcatactgc gaccatattc
     1321 ttcacccaac acccaggcat tataacggct ggcattaaaa ctctctgcac agccttccgt
     1381 gaagatgata tttttgtcag ggaaagcctc gtacacccgt ttcagatttt catacatcgg
     1441 tgtgccgcca ctccagtctt cataccagtg aaaaccgata ccccatgcat atttagccgc
     1501 ttccggatcg ttgaagtaag tttgagctct ttgataaata agatccctgt tatgatccca
     1561 cacaatgatc ttcttatctt tcaatccttc tttttccatg gtggggccca aatggttctt
     1621 cagaaaatct ctttcctctt ctgcggtata caaacaactt tcccagcgct gggtagccat
     1681 tggttcattc tgcacactga tcccccagac gggaacacct tctttttcat actctttaat
     1741 gaacttagta taatacatgg cccagctgtt ataaaactcc ggtttcagtt taccaccctg
     1801 cagcatatta ttattatctt tcatccaggc cggcggactc caggggctgg caaataaatc
     1861 caatttgcca cctgcagctt tcatcgcttc tttaataaat ggaattttat attttctgtc
     1921 atgatcaatg ttgaatgatt tcagcgctgt atcattatcg gcaacataag tgtacatatc
     1981 actgctgaaa tcgcagctat tgatatttgt acgggccact gtatagccaa tgcctttctc
     2041 tttatcaaaa aatgcctgca gtacttcctg ctgtttatct tttggaagtt tataaaatgt
     2101 ctctgcagaa gcatccgtca atgctgcccc tgtgccaaaa taggtttgaa acgtctttga
     2161 tggatcaaca aaaacacaga tctctgtttc tttcggctgt cccatttcct taaatacaag
     2221 agtatctgtt actgataaac gatacgctgt gctatcagca gtagtataca caataacttt
     2281 cttgccatcc gttgaaaaag atgcggcaga agatgttgta acaggttttt ctttgttatc
     2341 agaacaggag aatagtccta aggccgtaat aacaataaat agttttttca tacttgtcat
     2401 tataagttat aaaattacca aatgtaggtt cctactgtag cacccggcaa ttcggttgcc
     2461 gcccacttgc ctttgtactt aatattaaaa gaagtactgc tgccactatc atttaataca
     2521 atcaatactt tttttccatc cggtcttaca aatgccacac tatgaagact aacaccagtg
     2581 gtacttgcaa tacgaactga accggcaggt ataaatttgg aagcatgtgc aattatataa
     2641 taaccaacat tcctggttac tacagaagcg ttgatggtta aagcgccttt gcactccgtg
     2701 caaccgcccg gtgtatgtgg accataagaa gggtcattag ccaggttcca ttccaatgcc
     2761 gtcttactcc agttccgcat agaaccaata acgacattct tcaaatgcca tttcagatcg
     2821 ccgtcaaaac ttccgttagc acctgtccac tgttcagtaa aataaagatt cttatcagga
     2881 tgtgcggctt ttacgcctga caaaacaccg atctcacctg catataagtg aaaagcggaa
     2941 ccatcaataa atggttttgc agcagcatca ttcagaattg tgataggata attagggttg
     3001 tcgcaattat gatcccatat aatgatcttg gttttgatag ttgcagcctg gaaagccggg
     3061 cctaaatgat tttttataaa ttctgcttgt tgcaaggaag acattaccat gcttggatta
     3121 ttgcctccat gctgaggttc attttgaacg gtaatagcat caattttaat tcctctggcc
     3181 gccatcgcct gtatatattt gacaaaatac tgtgcatatg cagcatagta ttgtggtaat
     3241 aaacttcccc cgacagagct tccattatct ttcatccata ccggcgggct ccatggtgac
     3301 cccataatct taatctgtgg attgatagta agaatttgct ttaataccgg aaccaggtca
     3361 actgtatcga gtgataaact gaaattagcc agcgttggat ccgtttgccc ggcaggcatg
     3421 tcattataac taaaaacaga aggacttaaa tcagaagcac cgatacttat tctcaaatag
     3481 ctgataccga tagcatcatt tccgctgcca aataattcat tcagcaaaga tgaccgggaa
     3541 ggggcatcca tccggttaat gagataagcg ctaccgccag ttaaagtgta cccgaatcca
     3601 tctatttcct gaaattttgt tgtggaatca acttcgatat tggtaaaact attagctgtc
     3661 gtagtaaaat tgagtgccgt tggttgtttt tggagtaaaa cagaacggtc tcccttggta
     3721 atccagtatt caatggccgg agaaggtgat tgagtacctc caccagcatt gttcttggaa
     3781 caatttgatg aaagcgatag tatccctgcc aataaaaaag caggtaacaa aaattttgtt
     3841 tgcatattta ttattgtgag tgtaatcatt gctactaagg tgtttgtttc aggaatagca
     3901 acggcttatt attccgggca atgagcatga gtttactatt acctgctgta ttcaaccatt
     3961 ttatatccct tgcttccccg aaaaaggacg aaatagctgt ttggtaatta aatgcattta
     4021 ctgttctgtc aaaagaaaaa aacgttggca gcaaggcatc ataacggcct tcatagggta
     4081 ttactccaaa aaaatttcct gccgcgatat aaccagcctg cccggaaccc gccggagcaa
     4141 atgccattac cggggccaat tgcaattctg ccggtaattc cacttttttg aaattccctt
     4201 tcccgtcatt aataaaacag ctggagctca gcgtttctgc tttcagctcc aggtaatcct
     4261 tgaacagatc aaagaacata aaatcaactg ttttccctgc tacttcacta taagtaagat
     4321 aggctttctt caaaacagga agtggtcttt ccagttcatc ctttgccagg aaagtatatt
     4381 cttttccatt gacagtatag cacatgatct gctctgttga tccattgaga tcaaaatcct
     4441 tgacatagag ttttaatgga ccatttttac cggcaaataa ttttgagttg tgtccccagt
     4501 tcccagcaag tatatctgca aagccatctc cattcacatc tgttgtataa acagtttgcc
     4561 agagtcctgt tgattgaggg atttcggtca ctgaaaaaac acctttatta ttaataaaga
     4621 tctttagtgg catccactcg ccggtaacca caaggtccat ccagccatcc ttattcacat
     4681 cagtgaatga tgcagctgtt accattccca cagactgaag tggtatgata ttatcacctg
     4741 cataactgaa tttccctttc ccgtcattga gcatcaggta agatgtttgc ggtatgccat
     4801 aggcagtgga gctggcaagg tttccaacaa atatgtcagt atctccgtct ttatcaatat
     4861 ctgcaacagt tacacaggat ttattttcat agcgctgtgt aaaagcagcg gctgactttg
     4921 aaaattttcc ttttccatca ttcaggtaaa gcctgtcttc ctgtgacagg gcattggcac
     4981 ggggttcatt accaccactt acaacatata agtcaggaga cccgtcaccg tttgcatcaa
     5041 aaaaaacagc atccacatct tcgcatattg catcaggacc aaacacggct gtatcagcag
     5101 ctataaatga accattcccc tgttgaatca ttaaagtgcc gggctggtat ttagcaccac
     5161 aggcataaaa gtcatccaat ccatcgccat taacatccgc cactgctatt ttaggtcccc
     5221 tcgttgataa ggcatgtggg atcaggtatt gaacattaaa atcaacaaat tcattttcct
     5281 tatgcttcca gttgcagaca atattttgtg atacatcagt caataaagaa ggctgttgct
     5341 t
//
LOCUS       DAORTI010000189         5326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18812_length_5326_cov_7.219313, whole genome shotgun sequence.
ACCESSION   DAORTI010000189 DAORTI010000000
VERSION     DAORTI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18812_length_5326_cov_7.219313"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..452
                     /locus_tag="PLO70_15535"
     CDS             45..452
                     /locus_tag="PLO70_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75932.1"
                     /translation="MKHYHIISRIAIYLLAIVLFVFGIFHFMYPRDLLVYVPDFLPLG
                     IKWAYVVGGAFILVALCYLTNQFVKISSYLLFILLVIFILTIHLPNYNHAGDPEMRQL
                     ALINILKDTAIAAFALHIAAGAYHQHFHLENSD"
     gene            complement(638..1411)
                     /locus_tag="PLO70_15540"
     CDS             complement(638..1411)
                     /locus_tag="PLO70_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQZ75933.1"
                     /translation="MPFPDFADPSVWISLLTLSFLEIVLGVDNIIFISIVSNKLPESQ
                     QPKARSLGLLLAMVFRILLLLTITWIIKLTNPVFTIPFIEENGAPIGISWKDIILLLG
                     GIFLIAKSTLEIHHKLEKSATPKNKVVYTAFSAVILQIILVDAVFSFDSILTAIGLVD
                     NVIIMIIAVIISMLVMIAFAGAISAFINRNPTLQMLALAFLIVIGIMLIAQGFHQQIS
                     KSYIYTAMAFSLFVELLNMRLRRNVEKVQLNTGESEDEK"
     gene            complement(1547..2023)
                     /locus_tag="PLO70_15545"
     CDS             complement(1547..2023)
                     /locus_tag="PLO70_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75934.1"
                     /translation="MKKRKPLAVPFLLFTISTFFLSFIPVNKWGGDGFEVYLNNKLVL
                     QKYGNKMDNVQNLQLDKVSANDQLIIKYSHCGDIGKNRVITIKDGQNKVLKEFHYKDA
                     DNAVKVDYGISCPLKELLKLKKGTSNTFKLYYSSSQLPAGRLLTHICFDGQNTARL"
     gene            2183..2608
                     /locus_tag="PLO70_15550"
     CDS             2183..2608
                     /locus_tag="PLO70_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75935.1"
                     /translation="MQRLKPEVDIVKDLLEAVLVAQPESSFIQSLLYQYMERGGLSKK
                     QLQGLYGKASKIDSIPPGKLATLEAIILKRPTRYKSALPETAPLYTKDAAAGKIIETI
                     LSKYPQHKRVLFLQSKYDNNEVLTPPELAELQKFVKLLK"
     gene            2698..4347
                     /locus_tag="PLO70_15555"
     CDS             2698..4347
                     /locus_tag="PLO70_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75936.1"
                     /translation="MGQNLHRHFISKLHHAVDGNAQLAALLECSRLALSNPVLLKEYH
                     EVLLLFCAYPGNKKVRELAKEELNRLGQSIKKHLYNNRWQHVLAGSGLPFTELRCQYS
                     LEMVSWLLEKFPGQVKPAEAGATATAVRQTFQSLLPGIEFERSTHGDLNLWNRIKSLS
                     GHYFNVPSLKWLLQLIQQQQWPVLLRDQLYDNLNIFISWSLTGNSWNRSFLHWPVNNV
                     YCQSIKKKKVNSTLIIHQPVKPSLPLSEQEKKELIGTMRASLAFYYRETDPFTYADTT
                     AIELFALGQGLQVALIGMRKERRLSLESYVGFMAFKNGVPVSYGGGWLFGHRCKIGVN
                     IYPPFRGAESAWLFCQVMRLYYQRYNVHRFVVKPYQFGKGNPEGLKSGAFWFYYKLGF
                     RPVNEKIRNAAEAEWKKITAGKSYRTSLKTLKSFTASIKEWIVEKKMAAHPLAGELSS
                     QVSSMIRQEFQGDREQAIFTCTRKMKIFLGIRSMPQLSREEISVWQNWALLWSILPGH
                     HTWNSRQRKYWLQLFWLKTRGSERDFILAWQQYKDLWCAEA"
     gene            4553..5161
                     /locus_tag="PLO70_15560"
     CDS             4553..5161
                     /locus_tag="PLO70_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQZ75937.1"
                     /translation="MEEKHYTQLLSADELTYHVEACALNSRESQKVLYSSFYGYAMAI
                     CDRYVNNQDDALEILNDGFLKIFKEIHHYKPAYKDVISSFKGWLRKIMVYTAIDHFRK
                     YQKHQLVASLDDVVYHVPSVSDDAINKLSYDEIIRAVQDISPGYRAVFNLFVIEEFSH
                     EEIAGHLGISVGTSKSNLSKARRQLQKILFKQNITSIAKNAV"
BASE COUNT         1655 a          954 c         1119 g         1598 t
ORIGIN      
        1 aattttaaat attgtacctt ttacacatat taaaactttc agttatgaag cattatcata
       61 ttatttcgag gatagctatt tatttactgg ctatagtcct gtttgtattt ggtatttttc
      121 attttatgta tccccgggac ctgttggtat acgttcctga ttttttgccc ctgggtatta
      181 aatgggctta tgtagtgggt ggtgctttta tactcgttgc actttgttac ctgaccaatc
      241 aatttgtaaa gatctccagt tacctgttat ttatcctgct ggtgattttt atcctgacta
      301 tacatctgcc taattacaat catgccgggg atcctgaaat gaggcaactt gccctgatta
      361 atattttaaa agataccgcg attgctgctt ttgctttaca tatcgcagcc ggggcttatc
      421 atcagcattt tcacctggag aacagtgatt gagttgcaga tcgattttga gaaatatttt
      481 aaagcgatta aactgttgaa gtatatcagc agtttaatca ctttttttta accattatct
      541 atgtcagcat aaatacttta accttagctc aataaaataa tgcggaaaat acattctgta
      601 agcatggggt tttctgataa ataaattctc agcgtaatta tttttcatct tcactttcgc
      661 cagtattcag ctgcactttt tcaacatttc ttctcaggcg catatttaac agttcaacaa
      721 acagtgaaaa tgccatagct gtgtaaatat aacttttact gatctgttga tggaatccct
      781 gggcaataag cataatacca ataacaatca ggaatgccag cgccagcatt tgcagggtgg
      841 ggttcctgtt aatgaaggcg gagatcgctc cggcaaatgc aatcattacc agcatcgata
      901 ttatcactgc aatgatcata atgataacat tatctaccag gcctatggca gtcagtatgg
      961 agtcaaagga aaaaacagca tctaccaata tgatctgtaa aataacggca ctaaaggcgg
     1021 tgtaaacaac tttgttttta ggtgttgctg atttttccag tttatgatgg atctcgagtg
     1081 tgcttttggc tataaggaaa ataccaccca gcaacaggat gatatctttc cagctgatac
     1141 cgataggagc cccgttttct tcaatgaaag gtatagtgaa gacgggattg gtaagtttga
     1201 tgatccaggt tatcgtcaat aaaagcaata tcctgaaaac catagccagt aataaaccaa
     1261 gcgaccgggc tttgggctgc tggctttccg gcaatttgtt agaaactatt gatataaaaa
     1321 tgatattatc aactccaagt acaatttcaa gaaaactcaa tgtcagcaga ctgatccaaa
     1381 cggaaggatc ggcaaaatcg gggaatggca tttattttaa atttaaacgc aaatgtaaag
     1441 ctaaaactga acagtatttc cggtaaagcc aggaaacaga aaagccctga ttgcggtata
     1501 caaacagggc tattgattta aaaaagataa agcagagagg cttgtattaa agccgggcag
     1561 tattttgacc atcaaagcaa atatgcgtaa gcaatctccc tgcaggtaac tggcttgaag
     1621 aataatacag tttaaaagta ttggaagttc cctttttcag ttttagtaat tctttcaggg
     1681 ggcagcttat tccataatct acttttactg cattatccgc atctttataa tgaaattctt
     1741 tgagcacttt attctggccg tcctttatgg tgatgaccct gtttttacca atatcgccac
     1801 aatgagaata ttttataatc aattggtcat tggcagatac tttgtctaat tggagattct
     1861 ggacattatc cattttgttg ccatatttct gtagtaccag tttattattc aggtatactt
     1921 caaatccatc acctccccat ttattaacag gtataaaaga taaaaagaag gtactgatgg
     1981 taaacagtaa aaatggtact gcgaggggct ttcttttttt catggcaatt cttttttaag
     2041 gttaatgtaa atgaacattt acttgaatcc acaaaaaaag gattttgaca gacctgtaca
     2101 aaataccctt gacaaatgta gcggaatact tgatgtatag catggtaacg gctattgtcc
     2161 caatctttgt tatttttatg caatgcagcg acttaaaccg gaagtagata ttgtcaagga
     2221 ccttttggaa gcagtactgg tagcccagcc ggaatcctct tttatacaga gccttttgta
     2281 tcaatacatg gagcggggcg gactgagcaa aaagcaatta cagggtttgt atggcaaggc
     2341 ttctaaaatc gattcaattc cgccgggtaa actggctaca ctggaggcta ttattttaaa
     2401 aaggccaacc cgttataaat ctgcacttcc ggaaacggcg ccattatata ccaaagatgc
     2461 ggctgcagga aaaataatag aaacaatcct ttccaaatac ccacagcata aaagagtgct
     2521 tttccttcaa tcgaaatacg ataataatga agttctcact ccccctgaac tggcggagct
     2581 ccagaaattt gttaagctat tgaaataaga ggattctctg tatcatatca tgagaaatca
     2641 tgatcgttct cataagagta tcctggcagc ctgtatattt tcaattattg atttaacatg
     2701 ggccagaatc tgcatcgtca ttttatcagt aaacttcacc atgcggtgga tggcaatgca
     2761 caattagcag cactactgga atgcagccgg ctggcattaa gtaatccagt gttattaaaa
     2821 gagtatcatg aggtcttgct tttattctgc gcatacccgg gcaataaaaa agtgagggaa
     2881 ctggcgaaag aagaattgaa caggcttggc cagtctatta aaaaacacct gtataataat
     2941 cgttggcagc atgtattggc aggaagtggc ttaccgttta cagagttaag atgtcagtat
     3001 agcctggaaa tggtgagctg gttgttagag aaattcccgg gccaggtaaa accggcagag
     3061 gcgggagcaa cagcaacagc agtccggcaa acattccagt ctttgttgcc gggaattgaa
     3121 tttgaaagat caacacatgg tgatttaaat ctttggaatc gtattaaatc tttaagcggt
     3181 cattatttca atgtgccttc attaaaatgg ttattacagt taatacagca acagcaatgg
     3241 cctgtactat tgagagatca gttatatgat aatttgaata tttttatcag ctggtcgtta
     3301 acaggtaatt catggaaccg cagttttctt cactggccgg taaataatgt atactgtcaa
     3361 tcaataaaaa agaagaaagt aaacagtact ttaattatcc atcaaccggt aaaaccatca
     3421 ttaccacttt cggaacagga aaagaaagag cttatcggaa ctatgagggc atcactggct
     3481 ttttattacc gggaaacgga tccctttaca tatgccgata ctacggcaat tgaattgttt
     3541 gcattgggac agggactgca agttgcattg attggtatgc gcaaagaacg acgcctgtct
     3601 ttggagagtt atgtgggttt tatggctttt aaaaacgggg taccggtttc gtatggtggt
     3661 ggctggctgt ttggccaccg gtgtaagatc ggtgtaaata tttatcctcc tttccgcggt
     3721 gctgaatcag catggttgtt ttgccaggtg atgcggttgt attatcaacg ttataatgtc
     3781 catcgttttg ttgtaaaacc ataccagttt ggtaaaggaa acccggaagg attaaagagc
     3841 ggtgcctttt ggttttacta caaattaggg ttcagaccgg ttaatgaaaa aataaggaat
     3901 gcagctgaag cagaatggaa aaaaattaca gccggcaaat cgtaccgcac atcgctgaaa
     3961 acattaaaaa gttttactgc cagtattaaa gaatggattg tggaaaagaa gatggcagca
     4021 catccgctgg caggggagtt aagcagtcag gtcagttcca tgatccggca ggaatttcag
     4081 ggtgacaggg aacaggccat ttttacctgt acccgtaaaa tgaaaatatt cctgggaata
     4141 agatcaatgc cgcaattaag cagagaggaa atttccgtat ggcaaaactg ggcattgctg
     4201 tggtccattc ttcccggtca tcatacctgg aatagcaggc aacgaaaata ctggctgcag
     4261 ttattttggt taaaaaccag gggaagtgag agagatttca ttttggcctg gcagcagtat
     4321 aaagatttat ggtgtgcaga agcatgaagt cagtatgcct gtaaggaagc ttcctgcaca
     4381 acctaagaac ctacattaat tccgggttta ttccactgcg ggaattacat ccaatactcg
     4441 tcaattaaat aattctcctt cagatggtta tgccggactg gttggtggtt accaaaaaga
     4501 gaattaataa attaacttgt aaacaggcaa cctgaacact ttatactccg atatggagga
     4561 gaaacactac acacaattgt tatctgcgga tgaacttacc tatcatgttg aggcttgtgc
     4621 actcaatagc agggagagcc agaaagtttt atacagctct ttctacgggt atgctatggc
     4681 tatttgtgat cgctatgtca ataaccagga tgatgctctt gagattttaa atgacgggtt
     4741 tcttaaaatt tttaaagaaa ttcatcatta caaacctgct tataaggatg tgataagctc
     4801 atttaaagga tggctgcgga agataatggt ttatactgct atcgatcatt tccggaaata
     4861 tcaaaaacat cagctagtgg catcattgga tgatgtagtc tatcatgttc cttcagtcag
     4921 cgatgatgcg atcaataagt tatcttatga cgaaataata agggctgtac aggatatttc
     4981 acccggatac agggctgttt tcaatctttt tgtgatcgag gaatttagtc atgaagagat
     5041 tgccgggcac ctgggaatct cggttggtac gtccaaatca aacttatcga aagcaagaag
     5101 acaattacag aaaattttat ttaaacaaaa tattacatca attgccaaaa atgcagtttg
     5161 aagattttga taagaagatt aaagaagcag cagaacatca ccacccggcg tatgatgaaa
     5221 tggcatggga taaaatggag aagctgctgg ataagcatct tcctcaaaaa gaggataaga
     5281 agagaaggtt tatctttttc ttattgttgt ttttgttttt gggagg
//
LOCUS       DAORTI010000190         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18910_length_5302_cov_5.856108, whole genome shotgun sequence.
ACCESSION   DAORTI010000190 DAORTI010000000
VERSION     DAORTI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18910_length_5302_cov_5.856108"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1120)
                     /gene="holA"
                     /locus_tag="PLO70_15565"
     CDS             complement(122..1120)
                     /gene="holA"
                     /locus_tag="PLO70_15565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQZ75938.1"
                     /translation="MSVEKIISDWKKKIFKPVYWLEGEEEYFIDKAVDYAEHHILNES
                     EASFNLSVFYGKDANWADVLNACRRYPMFAERQVVLLKEAQQMKDVEKLEPYIETPLS
                     STVFVVSYKEKKLDARKKFAKLVKENGVLVTTKKMYDKDVPAWTQELVHSKGLTISPR
                     GLALLVDHIGNDLTRIENEIDKLSVNLGKGTNITEDDIEKYIGVSKDYNVFELQAALA
                     AKDLARTIRIIQYFEANPKAGPIQLILPSLYSFFSKVFMIFGAGTNDEKTIASSIGVN
                     PYFMKDYMQAARLYSYPGVEKVLLLLHQYNLKSIGVGSTATEDASLMKEMVVKMLS"
     gene            1338..2312
                     /locus_tag="PLO70_15570"
     CDS             1338..2312
                     /locus_tag="PLO70_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75939.1"
                     /translation="MTNTNYPSATTTPETQPNKNRNSKNLIIGLLAAGLLGTWGYLLW
                     DKNKSGETISKQQAQIAVLDSAKTDIQMEFDNALARLDSVTGANNNLQGQLTDRQKEI
                     DAKKSEIRKILNDKNATQAQLAKARTMIADLNDKITGLEAEVTRLTGENQELTAANTT
                     LKDEKTALETNLTTTKSEKETLEKTVDVGSTFSASNIQVTPVDEKKSGKEKSTSTAKR
                     VDKLVVSFDVENRIAKSGPADMYLIVTAPDGKVISDAGSILNTRQDGEKTFTARIPVN
                     YEQGTRKPVQFPIKQEDFKTGNYKVEIYHNGFKIGEGVRSLKKGGIFG"
     gene            2495..3643
                     /locus_tag="PLO70_15575"
     CDS             2495..3643
                     /locus_tag="PLO70_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75940.1"
                     /translation="MLHTNYRYAVLAFLLFSNTLVVVAQNTVQPKYNNSSVYTGIEVG
                     SKGVKMSIIEIGKNAQSTGAFNILKDTSVNTDFISFSDPTFTATLDGLYGLYTKATNE
                     YKIPRKRIFTVISSGVKMQAEKDKKTRNVKALIDSFRLRIGEPERIVEVVDVMQEARL
                     SHLGIVPDKRRFTTFLIDIGSGNTKGGFFPDGMNTENFKLFQLNWGTKSTYNETEKRC
                     DGDMSMPNFSKNLSRVLMNAENADIIYAVNASGAYPLSDNIAVSGGIAWATATLMHPE
                     LIDNPVISITFDELVKFNEKLLKNFPALSADFLTSTIKNPEQKKQVMEEIKRVHKVFD
                     QKSLMSGAGLLWKIMRQFESAFETKQFYLVKNGQVGWVSAYVDETISN"
     gene            3680..4606
                     /locus_tag="PLO70_15580"
     CDS             3680..4606
                     /locus_tag="PLO70_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75941.1"
                     /translation="MKDIKGFLLVMLSVGLVGTWVYHLYDKTQYTTNRKEIFIKDSIA
                     VAEAVQDSLQKIYTASLNSLDLKLDSTRSGADSLKKQLNKKLGEINKLRAEINSILKN
                     SGASQSDMTLARKKIAELQVLVEELKGEKTSIEEEKKRLSDVMTQLNGEITDLQQTMK
                     KLGDENKLLAEKVDRASVFVASEIRLSPVTLKNDKEQETNIAKKVSKLIVSFAVQNNV
                     NDYTNTEVYIIITQPDGKVLTPDVWESATQMTTQSGDKKSYTRKIKFEYQKGESKQLT
                     FSLNADDYQQGRYTLQLYHNGHMIGQVVKTLN"
     gene            complement(4817..>5302)
                     /locus_tag="PLO70_15585"
     CDS             complement(4817..>5302)
                     /locus_tag="PLO70_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75942.1"
                     /translation="ANFELYSPYSSTNVSYVQNSDDNVKSIGWGIGLEYQLIKRYVLY
                     GNVFSDQLKDVDPAVVTFFNAPKYRFNIGLRNENVFNNIGFNVVFKWQDNNYYEGTFV
                     SGTLPYFGWWDAQVTYRPPTTKSTFRIGGTNLGNQYQRTGFGSPAVGGLYYISYGYNL
                     F"
BASE COUNT         1768 a         1030 c         1029 g         1475 t
ORIGIN      
        1 ggaaacaaat aatgacaata ctattttttt cataattcta tttaattgct caacaggatg
       61 aatactaaat tcaaaaagtt gcacaaagaa agggaaatta aactaatgac agagttgctg
      121 ttcagctaag catttttacc accatttcct tcatcaagga agcgtcttcc gtggctgtac
      181 ttcctacacc gatactcttc aggttgtatt gatggagcaa tagtaatact ttttcaacac
      241 cgggataaga gtaaagccgg gctgcctgca tataatcctt cataaaataa ggattgaccc
      301 ctatactgct ggctatagtc ttttcatcgt tggtacctgc cccgaaaatc ataaatactt
      361 tgctgaaaaa actataaaga gatgggagta tcagttgtat cgggcctgcc ttggggttcg
      421 cttcaaagta ttgaatgata cggatagtac gggcaaggtc tttggcagcc agtgctgctt
      481 gcagctcaaa tacgttgtaa tctttactga ctccaatata tttttcaata tcatcttcag
      541 taatgttggt ccccttacca agattaacag aaagtttgtc aatctcattt tctatccttg
      601 tcaggtcatt cccaatatga tctaccagta aagccaatcc cctgggtgag atggtcaatc
      661 ctttggaatg caccaattcc tgggtccagg ccggtacatc tttatcatac atttttttgg
      721 tagtaaccaa tacaccattt tccttaacca gtttggcaaa ttttttacgg gcatccagtt
      781 ttttctcttt ataagagact acaaaaaccg tcgaacttag cggagtttca atatagggtt
      841 ccagtttttc gacatccttc atttgctgag cctcttttaa cagcaccacc tgtctttcgg
      901 caaacatcgg gtaacggcgg caagcattca atacatctgc ccaattagca tctttgccgt
      961 aaaaaacgga taaattaaac gaggcctcac tttcattcaa aatgtggtgt tcagcataat
     1021 ctacggcctt atcaataaaa tattcctcct ctccttcaag ccagtaaacc ggcttaaata
     1081 ttttcttttt ccagtcggaa attatctttt ccacactcat aagctgctaa gatacaggct
     1141 ttcaggatgc ctggggttca aaaaagacaa ttatttagaa acagccggat tcattctggc
     1201 acggttttcg gaaaataggt cccggaagat tttaacacga tttatccggt tcgcttgcaa
     1261 ccacctgact aatttgcagc ggatcaattt aaaaaagcta ctaatctaaa attttaatta
     1321 ctaaaatccc tttttgtatg acaaacacga attatccttc ggctaccacc acccctgaaa
     1381 ctcagccgaa taaaaacagg aacagcaaaa atttaataat tggcttgctt gcagcaggtc
     1441 tccttggcac atggggttat cttctctggg acaaaaataa atccggagaa actatctcta
     1501 agcaacaagc tcaaattgcc gttttggact ctgccaagac ggatatccag atggaatttg
     1561 acaatgcatt ggcaagactg gattcagtta ccggtgctaa taacaatttg cagggtcagt
     1621 taacggacag gcaaaaagaa attgatgcta agaaatctga gatccggaag atcctgaatg
     1681 ataagaatgc aacacaggct cagttagcca aagcaaggac aatgattgca gacctaaacg
     1741 ataagataac aggtctggaa gctgaagtaa cccgcctcac cggtgaaaac caggagctga
     1801 ctgcagccaa tacaacgtta aaagatgaga agactgcatt ggaaacaaac ctgacaacta
     1861 ccaaatcaga aaaagaaacc cttgagaaaa cagtagatgt gggttctact ttcagcgctt
     1921 ccaatataca agtaacacca gttgacgaaa agaaaagtgg aaaagagaaa agcacttcta
     1981 ctgctaaaag agtagacaaa ctggtagttt cttttgatgt ggagaaccgc attgcaaaat
     2041 caggtcctgc agatatgtac ctgattgtta cagctcctga tggaaaagta atctctgatg
     2101 ccggaagcat tttgaatacc cgccaggatg gtgaaaaaac attcacggca aggatacctg
     2161 ttaattatga gcagggaacc cgtaagcctg ttcagttccc gatcaaacag gaagatttca
     2221 agacaggaaa ttataaagta gaaatatatc ataatggatt taaaattggt gaaggtgtaa
     2281 gaagccttaa aaaaggtggt atattcggtt aaagcagata actcctgcta caaaaagccg
     2341 cttcgtattt aacgaggcgg cttttttatg attaatgata tttaacagga ataacattta
     2401 gccacatttt ttacacatta ttgatgcgcc aaaatagaat aaagtaaaac tgcgtggtat
     2461 tgtagtattt ttatcaggaa gtaaattcaa aaatatgctg cacacaaatt accgttatgc
     2521 ggtattagct ttcctgcttt tttcaaacac tttagttgtt gttgctcaaa atactgtgca
     2581 gcctaagtat aataattctt cagtttacac gggtattgaa gttggttcta aaggcgtcaa
     2641 aatgagcatc attgaaattg gtaaaaatgc acaaagcacc ggagcattta atattcttaa
     2701 agacacttcg gtcaatactg atttcatcag tttctcagac ccaactttta ccgccacact
     2761 ggatggactg tatggcttgt acacaaaggc taccaatgag tataagatac ccaggaaaag
     2821 aatatttacc gtcatcagca gcggagtaaa gatgcaggca gagaaagata aaaaaacaag
     2881 aaatgtaaaa gcattgattg attccttccg gctcagaatt ggtgaaccgg aaagaattgt
     2941 ggaagtcgtt gatgttatgc aggaagcccg gctttcgcac cttggtatag tacctgacaa
     3001 aagacgcttt actacttttc tgatcgatat tggaagtggc aacaccaaag gaggcttttt
     3061 cccggatggc atgaatactg aaaactttaa attattccag cttaactggg ggactaaaag
     3121 tacttataac gaaacggaga agagatgcga tggagatatg agtatgccca atttctctaa
     3181 aaatctttcc agagtattga tgaatgctga aaatgcagat attatttacg ccgttaacgc
     3241 cagtggcgca taccctctta gtgacaatat tgccgtaagt ggtggtatag cctgggcaac
     3301 tgctacgttg atgcatcctg aattgattga caatcctgtt atctccatca cttttgatga
     3361 gttggtaaaa ttcaatgaaa agcttttgaa gaacttcccg gctctttcag cagattttct
     3421 cacttctact attaaaaatc ctgaacaaaa aaagcaggta atggaagaga ttaaaagagt
     3481 acataaagtc tttgatcaga aatcactgat gtccggtgcc ggtcttttgt ggaaaataat
     3541 gcgccagttt gaatcagctt ttgaaaccaa acaattttac ctggttaaga atggacaggt
     3601 aggttgggta tctgcatatg tagatgaaac aatctctaat taatttacag taaagtaatt
     3661 aataaatcag cttccaccta tgaaagatat taaaggcttt ttattagtaa tgctttctgt
     3721 tggattggtg ggtacctggg tttaccacct gtatgacaaa acccaataca ctaccaatag
     3781 aaaagagatt tttattaaag attctattgc ggtggcagaa gctgtacagg attctttaca
     3841 aaaaatttat accgcctcac tcaatagcct tgacctgaag ctggattcaa cccgctccgg
     3901 agctgattct ctcaaaaaac aactgaataa aaaactaggg gaaatcaata aactgagagc
     3961 cgaaattaat tccattctaa aaaatagtgg cgccagccag tctgatatga cactggcccg
     4021 gaagaaaata gcagagcttc aggtactggt tgaagaactg aaaggagaaa agaccagtat
     4081 tgaagaagag aaaaaaagat taagtgatgt aatgacacaa ttgaacgggg aaattacaga
     4141 tctccagcaa acgatgaaaa agctgggcga tgaaaataag ttgcttgcag aaaaagtaga
     4201 ccgtgcatct gtatttgtgg cttcagagat cagactttcc ccggttactc tgaaaaatga
     4261 caaggaacag gaaaccaata ttgctaaaaa ggtaagtaaa ctaatcgttt cttttgcggt
     4321 gcagaataac gtgaatgatt atactaatac tgaagtctat attattatta cgcaaccgga
     4381 tggcaaagta ttaacaccgg atgtttggga gtcagctaca caaatgacca cacaaagtgg
     4441 cgacaaaaaa agctacaccc ggaaaataaa atttgaatac cagaaaggtg aatcaaagca
     4501 attgacattt tctctaaacg ctgatgatta ccagcagggc cggtacacct tgcaacttta
     4561 tcataatgga catatgatag gacaggttgt gaagacattg aattgatact gacgactatt
     4621 ttatttacaa acaaagtcag ctatattata cctgaagacc gtttaaggca gcgatcaact
     4681 tcttcatcta tcccgaagct tacactttct ccaatagatc tcggatagta tttcaattat
     4741 tgtgattaaa taaattcaaa aaataaggcc tcccgaagga ggcctttata cttagaaccc
     4801 aatttcaatt cttttatcag aacaggttgt aaccataact gatataatat aaaccaccaa
     4861 cagcagggct accgaaacca gttctctggt attgatttcc aaggttagta ccacctatcc
     4921 tgaatgtgct cttcgttgtt ggaggacgat aagtaacctg tgcatcccac caaccgaagt
     4981 aaggcaatgt tccacttacg aaagttcctt cgtagtagtt attatcctgc catttgaaca
     5041 ccacattgaa accaatattg ttaaatacat tttcattacg caagccaatg ttaaaacggt
     5101 acttgggtgc attgaagaaa gtaaccacag cagggtcaac atctttcaat tggtcagaga
     5161 atacgttacc atataatacg tatcttttga tcagctggta ttcaagaccg ataccccaac
     5221 cgatagattt tacgttatca tccgaattct gaacatatga tacgttggtg ctgctgtagg
     5281 gagaatataa ctcaaaatta gc
//
LOCUS       DAORTI010000191         5298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_18925_length_5298_cov_5.700744, whole genome shotgun sequence.
ACCESSION   DAORTI010000191 DAORTI010000000
VERSION     DAORTI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_5298_cov_5.700744"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLO70_15590"
     CDS             complement(<1..261)
                     /locus_tag="PLO70_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75943.1"
                     /translation="MEAKDIKELLVTELETTKAAILKVADDNAKSEYKKMSDLVDEKF
                     AKLNQLPADVQPEMVTKAIADIKTIVDDWADMEKLVKSGRFAA"
     gene            complement(266..979)
                     /locus_tag="PLO70_15595"
     CDS             complement(266..979)
                     /locus_tag="PLO70_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HQZ75944.1"
                     /translation="MAEYILKQPNFLAASIKDMDMKEGIVTGYASSFDTLDADRDIIM
                     QGAFSKTIKEQGPKSSQPRIKHLLNHNTSQPIGNPLSLMEDAKGLLYESKVGTNAVAQ
                     DFLKMVDSGLITEHSIGFSTVRKTITNPDADWREQTTQIHEVKLYEFSSLTAWGANQN
                     TPLIGVKSLKTVEDRIANLIKAIDGGTFTDTTFLFLQDELLFLQKAFKDITTHAAHAP
                     EPDIEAQIKEAFQLFKQKN"
     gene            complement(1100..1807)
                     /locus_tag="PLO70_15600"
     CDS             complement(1100..1807)
                     /locus_tag="PLO70_15600"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HQZ75945.1"
                     /translation="MTPKEQKEFWLKFHRFQMRYELMYTPKINKVLKAQVQQYIKTKD
                     TIYVRSGELYALLMDLYTTTGTAWAYQTRGLLSKKAGGQMGFSERVVSIMRQIFEFEL
                     LSTAENITQTTIRLIQEVLTEAALEGWSFDEIVKRLVSPDMTAKRARLIARTETVNAA
                     NAGSMANLKAAGATKKIWIAARDNRTRPHHREVNQTVIGIDELFKVGDSFMSHPGSKE
                     GSAAEVCNCRCAVAGVV"
     gene            complement(1940..3280)
                     /locus_tag="PLO70_15605"
     CDS             complement(1940..3280)
                     /locus_tag="PLO70_15605"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HQZ75946.1"
                     /translation="MDIFGIKALRQQNQQLINSVKALQQNNIVTAVSNFRTMIFPNWM
                     AVKEIDAFIIFDDVYSVVSRLATSAAQIPLVAYNESNKEDLLPTDPMAIFLNTLSFEQ
                     REEMYLWVFLGECFIYKDALKLGPNKGKLKTAFLHPAYMSIEQEVNFPYRITGYRYQD
                     TSTTFTLLPEEVIYIKYGKNPSTNYNERNRGLGPIKALAQRLTRLQANMSASVSQMQN
                     GGVPSIVYDKTPGIDQDRGSGGASLNNEVTVMGQHKDNFSRFLRNPENKGAPYFAAGE
                     MGVIPLGLSLVELDALTQSGVDFDKICNAYSVSSLLFNSQNRGTFNNTGEMRKDMYTN
                     AILPNVRKVCDCLKFGTIDVFGQGKGVKPDLSGIPELQDDIKKKADGWAAMPAIVINE
                     MRVSLGQDASTDPMADKLLIKTGYQLSEDLDMEVDPIDNTANDYGKTDTDNSGE"
     gene            complement(3291..4670)
                     /locus_tag="PLO70_15610"
     CDS             complement(3291..4670)
                     /locus_tag="PLO70_15610"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HQZ75947.1"
                     /translation="MKSEPLYLPFDTHGNEKQKDCARAWVDDSITDIYYGGAKGGAKS
                     FTGVNLIFGDALTYPDTRYFISRKKLNDLRKFTLPTISKVFQSWGIKQEKYCKFNGQD
                     NFFECTNGSKILFLEAAYMPSDPLYERFGSMEFTRGFIEEAGEFDLPAKNNLSASIGR
                     WKNDHYNLTRKLLQTGNPSKNYTYRDYYIPFRNGTLKPHQKFIQAFIQDNHMIDKGYY
                     EQLDQSLSTNEKQRLLFGNWEYDDDPSALIQYEKILDIFTNIHVPAGDRFITSDIARL
                     GGDKIVIIEWDGWRGKVKHYSKQTLDVTTTQISAAMMRNSTGKSGTLLDSDGMGGGPV
                     DMLGVKGFINNSRAIPIPGGEFDSKGNPIPENFDNLKSQCYFKLAERINKNGLYLECE
                     TEEVKQWIIEELEQVKQKSLDSDMKKGVMPKDKIKELIGRSPDFSDTIMMREYFELKP
                     KIGFRAALY"
     gene            complement(4660..5244)
                     /locus_tag="PLO70_15615"
     CDS             complement(4660..5244)
                     /locus_tag="PLO70_15615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQZ75948.1"
                     /translation="MIINHTELLNALIQKYGLKSYLEIGVQNPANNFDKVRLPSTSKV
                     GVDPECDSFNILPETSDKFFEHCSRVFDLIFIDGFHEKDQVKRDFENSLRCLSDNGFI
                     VIHDVLPENESGTIVPRETKQWWGDIYKWAMLICSYKDIRYKTFNIDNGCMVVWKYPG
                     EMNDYGKIIAADMNWQTYLKIGHVLMNVTSTVEI"
BASE COUNT         1451 a         1138 c          942 g         1767 t
ORIGIN      
        1 tgcagcaaaa cggcctgatt taaccagttt ttccatatca gcccaatcat caacgattgt
       61 tttaatgtcg gcaatagcct ttgtaaccat ttcaggttgc acatcggcag gtaattggtt
      121 taatttggca aatttttcat ctaccaaatc gctcattttt ttgtattctg acttagcgtt
      181 gtcatcagcc acctttagga tagcggcttt ggtagtttcc agttctgtaa ctagcaactc
      241 ctttatgtct tttgcttcca tttttttagt ttttttgttt gaataattga aatgcctctt
      301 ttatttgcgc ttcaatatcc ggctcaggtg cgtgtgcggc gtgagtggta atatctttga
      361 atgctttttg taaaaataat aattcatctt gtaggaataa aaacgtggta tctgtgaaag
      421 taccgccgtc aatagccttt ataaggtttg caattctgtc ctcaacagtt ttcaaactct
      481 taactccaat aagcggagta ttctgatttg cgccccaggc tgttaaagat gaaaattcat
      541 atagctttac ctcatgtatt tgtgttgttt gttctcgcca gtctgcatca gggtttgtaa
      601 ttgtttttct tacagttgaa aatcctattg agtgttctgt aatcaatccg ctatcaacca
      661 tctttaaaaa atcctgtgca actgcattgg taccaacttt actttcatac aacaatcctt
      721 ttgcatcttc cattaatgat aatgggttac caatcggctg tgatgtatta tgattaagta
      781 aatgctttat cctgggttga gaagatttag ggccctgctc ttttattgtt ttggaaaatg
      841 cgccctgcat gataatatcc cgatctgcat ctaatgtgtc aaaactggat gcgtaccctg
      901 taacaatgcc ctccttcata tccatatctt ttatggatgc ggctaagaaa ttgggttgtt
      961 taagtatgta ttctgccatc ttgcaggtaa agttatataa aaatgcaaca atgttgcaaa
     1021 tttattttta aatactccga aacagcaaaa acccccgtag aaacaggggt cgcaaaacca
     1081 acacatgaag aaaagtattt tacactaccc cagccaccgc acagcggcaa ttacaaactt
     1141 ctgcagcact accttctttt gaacctggat gactcataaa agaatcgccc accttaaaca
     1201 attcatcaat accaataaca gtttgattaa cttctctgtg atgtggccgg gtgcggttat
     1261 ccctggcggc aatccatatc tttttagttg caccggcagc ctttaagttt gccatacttc
     1321 cggcattggc agcattcaca gtttcggtac gggcaatcaa ccgggcacgt ttggcagtca
     1381 tatcaggtga aacaagtcgt ttaactatct catcaaatga ccacccctcc aatgctgcct
     1441 cagttaaaac ctcttgtatt aaccggatag tggtttgcgt tatattttcg gcggttgata
     1501 ataattcaaa ctcaaatatt tgccgcatga tagaaacaac cctttcgcta aatcccatct
     1561 gtccgcctgc ttttttggat aataaccctc ttgtttggta ggcccatgct gtgccggtgg
     1621 tggtatataa atccataagt agggcatata attcaccgga tcttacataa atagtatctt
     1681 ttgttttgat gtactgctgt acctgcgctt tgagaacctt atttattttt ggggtgtaca
     1741 ttaactcata ccgcatctga aacctgtgga atttcagcca aaattctttt tgttcttttg
     1801 gtgtcatttg agttgtgttc tcggttcata ttgctgtagt gaaagcgatc tgattaattc
     1861 ctctaattgc aatcgcaact gaatacgctg cctcatttga tcattccgtt ttgtttgaca
     1921 ggtaggatca agcggtatgt tattctccac tattatctgt atccgttttt ccgtagtcat
     1981 tagctgtatt atctattgga tcaacttcca tatctaaatc ctcagaaagt tggtaaccgg
     2041 ttttaattaa taacttatcc gccataggat ctgtgctggc atcctggcca agtgaaacgc
     2101 gcatttcatt tatcacaata gctggcatag ctgcccagcc atcagctttt ttctttatgt
     2161 catcctgcaa ttccgggata ccacttaaat caggcttaac acctttgccc tgaccaaaca
     2221 catcaatagt accaaacttt aaacaatcac atacctttct cacatttggt aggattgcat
     2281 ttgtgtacat atctttgcgc atctcaccag tgttattaaa agtacccctg ttttgcgaat
     2341 taaatagcaa cgatgaaact gaatatgcgt tacatatttt atcaaagtca actcctgact
     2401 gtgttagcgc atccaactca accagtgata aacccaatgg gataacaccc atttcaccgg
     2461 cggcaaaata cggagcccct ttattttccg ggttacgcag gaaccggctg aaattatctt
     2521 tatgctgccc cattaccgta acctcattat tcaatgatgc cccaccacta ccacggtcct
     2581 ggtcgattcc tggagtctta tcataaacaa tggaaggaac tccgccgttc tgcatctgtg
     2641 aaactgaggc actcatattc gcctgtagcc tggttaacct ttgagccagc gcctttattg
     2701 gtcctaatcc acggttgcgt tcattataat ttgtagatgg gtttttgccg tactttatat
     2761 agatcacttc ctccggcagt agtgtgaatg ttgttgatgt gtcctggtac ctgtaaccgg
     2821 ttattctata agggaaattt acttcctgct cgatactcat gtatgcagga tgcaaaaagg
     2881 cagtttttaa tttgccttta tttgggccaa gttttaaagc atctttataa atgaaacatt
     2941 cacctaaaaa tacccataaa tacatttctt cacgttgttc aaaagatagt gtatttaaaa
     3001 aaatagccat cggatcagtg ggtagtaaat cttctttgtt tgattcattg taagccacca
     3061 atggtatctg cgctgctgat gtagccaaac gggaaacaac ggaataaaca tcatcgaaaa
     3121 taataaaagc atcaatttcc ttaactgcca tccagtttgg gaatatcatt gttcggaaat
     3181 tagaaacagc cgtaacaatg ttattttgtt gtaatgcctt aactgaattt attaactgtt
     3241 ggttttgctg cctgagtgct tttattccga agatatccat tgtagtaaat ttaatataat
     3301 gcagcccgaa acccaatttt aggctttaac tcaaaatatt cacgcatcat aatagtatct
     3361 gaaaagtcag gtgaacgccc aataagttct ttaattttat cctttggcat aacacctttt
     3421 ttcatatcac tatccagaga tttttgttta acctgctcca attcctctat gatccactgc
     3481 ttcacttctt cagtttcaca ttccaaatat aaaccgtttt tattaattct ttcagctaat
     3541 ttaaaatagc actgactttt taggttatca aagttctcag ggattgggtt tcctttactg
     3601 tcaaactcgc ccccagggat agggattgca cggctattat ttataaaacc ttttacacca
     3661 agcatatcaa caggaccacc gcccatgcca tcagaatcaa gcaatgtacc gcttttaccg
     3721 gtgctatttc gcatcatagc ggcgcttatt tgagtagtgg taacatccaa tgtttgttta
     3781 ctataatgct ttactttgcc acgccaccca tcccattcaa tgatcacaat cttatcgcca
     3841 ccaagccggg caatatcgga ggtaataaat ctatctcctg caggaacatg gatattggta
     3901 aaaatatcaa gtatcttttc atattgaatt aacgctgacg gatcatcgtc gtactcccag
     3961 ttaccaaaca acaagcgctg cttctcgttg gtagataatg attgatcaag ctgttcataa
     4021 tatcctttat caatcatgtg gttatcctgg ataaaagcct gtataaattt ttggtgtggt
     4081 tttaaagttc cgtttcgaaa cggtatgtag taatcccggt aagtataatt ctttgaaggg
     4141 ttaccggttt gcagcagttt acgggttaaa ttataatggt cgtttttcca acggcctatt
     4201 gatgcagata ggttgttttt tgccggtagg tcaaactccc cagcttcctc aataaatcca
     4261 cgggtaaatt ccattgaacc aaaacgctca tacaacggat cactgggcat atatgcagct
     4321 tctaaaaaaa gtatcttact gccgtttgtg cattcaaaga agttatcctg cccgttaaac
     4381 ttgcaatatt tttcctgctt tattccccac gattgaaaca ccttgctaat cgttggtaat
     4441 gtaaatttac gcaggtcatt cagttttttc ctggatataa aataacgggt atctgggtaa
     4501 gtaagggcat caccaaaaat aaggttaacg ccggtgaatg atttagcgcc accctttgca
     4561 ccaccatagt aaatatctgt aatactgtca tctacccatg cacgggcgca gtctttttgt
     4621 ttttcgtttc catgcgtgtc aaatggcagg tatagtggtt cagatttcaa cggtggaggt
     4681 tacgttcatt aaaacatgac ctattttaag gtaggtttgc cagttcatgt ctgcagctat
     4741 tattttgcca tagtcattca tttctcctgg gtacttccat acaaccatac acccgttatc
     4801 aatgttgaat gttttatacc gaatatcctt ataagagcaa atcagcatcg cccacttgta
     4861 aatatcaccc caccactgct tcgtttcgcg gggaactatg gttccggatt cgttttccgg
     4921 cagcacatca tgtattacta taaacccatt atccgaaagg cagcgtaatg agttttcaaa
     4981 atcacgtttt acctggtctt tttcgtggaa accgtcaatg aagattaggt cgaatacccg
     5041 gctgcagtgt tcaaagaatt tatctgatgt ttcaggtaaa atattaaaac tatcgcactc
     5101 agggtcaacc cctactttgc ttgtagatgg caatctcacc ttatcaaaat tatttgccgg
     5161 gttctgtacc ccaatctcca aataagattt taacccgtac ttttgaatca gtgcgttaag
     5221 taattcggtg tgattaatta tcatcaatgc ctatctttaa aatgttttat atatttaaat
     5281 tcctttgact gactgtaa
//
LOCUS       DAORTI010000192         5241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_19210_length_5241_cov_6.894138, whole genome shotgun sequence.
ACCESSION   DAORTI010000192 DAORTI010000000
VERSION     DAORTI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19210_length_5241_cov_6.894138"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /gene="hutU"
                     /locus_tag="PLO70_15620"
     CDS             complement(<1..1424)
                     /gene="hutU"
                     /locus_tag="PLO70_15620"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQZ75949.1"
                     /translation="MSTLTKRYDAIKYKTPTGTKLSCKGWIQEAALRMLLNNLDPEVA
                     ERPDDLIVYGGRGKAARNFEALDNIIAALKIVENDETLLIQSGKPVGILNTHKDAPRV
                     LISNSQLVPNWANWKHFDELEKKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAALADK
                     HFNGSLKGTLNVTAGLGGMGGAQPLAITMNEGVALIAEVEEWRIDKRIETKYLDEKHT
                     SIDEAINAATHYRREGEARSIGVLCNAVDLLDRLIQRDIIPDTLTDQTSAHDPLIGYV
                     PHTLSNEQANVIRQENPEEYIRRSYESMHLHVQLMLQLMDKGAITFDYGNNIRARAQE
                     FEQKAESLTPKALSVKPSALGRCFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPNDIA
                     VTDEVIMNMFPENKGLLRWMQMAKEKIAFQGLPARICWLGQGEREKAGLAFNELVRTG
                     KVKAPIVIGRDHLDTGSVASPNRET"
     gene            1576..2283
                     /gene="deoD"
                     /locus_tag="PLO70_15625"
     CDS             1576..2283
                     /gene="deoD"
                     /locus_tag="PLO70_15625"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQZ75950.1"
                     /translation="MSIHISAQKGEIAKTVLLAGDPLRAKYIADNYLQDVLLVSQTRN
                     AFYYTGTYKGKPVTVGASGMGCPSIGIYSYELFTEYEVDTIIRIGTCGAYTTKLKLFD
                     LVNVDYAASESTFARYAWGIKDQCIRHQGKAFKTIKKTATELNIALKNCNIHSSDIFY
                     RKDSNTPKVATANKCLAVEMEAFSLFANALHLGKTAATLLTVSDIIPTHEKITADQRE
                     TALQPMMVLALETVVKL"
     gene            complement(2526..2870)
                     /locus_tag="PLO70_15630"
     CDS             complement(2526..2870)
                     /locus_tag="PLO70_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQZ75951.1"
                     /translation="MFIYKQHHYYVYIVTNPERTVLYTGVSNNLSQRLKEHWANRGQP
                     KTFAGKYYCYNLIYYEDFTYINNAIAREKEIKGWRRQKKLDLIKTMNPDWTFLYPTVC
                     GGWPPEEVVKRS"
     gene            complement(2927..4504)
                     /gene="hutH"
                     /locus_tag="PLO70_15635"
     CDS             complement(2927..4504)
                     /gene="hutH"
                     /locus_tag="PLO70_15635"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQZ75952.1"
                     /translation="MSNPFRYGTDQLTVEKTIAIASGKLKAVLSEEAIKKIKASQQYV
                     QQIVAENKTVYGVNTGFGILANTPISKEDTATLQYKILQSHSVGVGDPIAVEVAKIML
                     ITKVHALAQGFSGVQLSTLERITWHIENNVIPVVPEKGSVGASGDLAPLAHLFLPLIG
                     LGEVWAKNGKETIRKNTSEVLKEKGLQPVQLGPKEGLALINGTQFILSFAVKAVQRMH
                     NCLEAADIIGAMSLEALTGTKAPFDERLHELRPYKGNKLVAQRLRLLLNDSAIMQSHM
                     DCGRVQDPYSLRCMPQVHGASRNAWLHLKELTEIELNAVTDNPIILGADDTISGGNFH
                     GQPMALPLDYCCFAAAEIGNISDRRCYLLLEGKWGLPMLLMKDVGLNSGFMIPQYTTA
                     ALVTENKTLCFPASADSIPTSLGQEDHVSMGSISGRKLNRVLDNLEFILAIELLSACQ
                     AIEFRRPLKSSVLLEFAHDYVREYVGFAEEDRIFADDINKVAGLIKDFSFVEKVNEVA
                     ASKGIELNSGFQEFSAL"
     gene            4621..5136
                     /locus_tag="PLO70_15640"
     CDS             4621..5136
                     /locus_tag="PLO70_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75953.1"
                     /translation="MLRLFSLLASLLIMLTTGAQSLKKYPVSNSGCTAYTYCEIKYEL
                     SKSADSSSIYSGECTKEDVNYGIICVKLLNPITELPLAEDMMIAYADYLKTSFEITKS
                     MGYGRGHLLNNKENTRGIIDYWEDGEKNKWKIKAWTDGKFIGFLYVYSKKELPEAKVN
                     AFLDSFRLPGQ"
BASE COUNT         1397 a         1249 c         1092 g         1503 t
ORIGIN      
        1 gtttcccggt tgggagaggc cacacttccg gtatcgagat ggtcacggcc gataacgatc
       61 ggcgctttta cttttcctgt cctcaccagt tcattaaaag caagacctgc tttttctctt
      121 tcgccctgtc ctaaccagca aatacgggcc ggcaatccct ggaaagcaat tttttctttg
      181 gccatctgca tccagcggag cagaccttta ttttcgggga acatgttcat gatcacttca
      241 tctgttacag caatatcatt cgggtctcca ctcagtgccg cccagcggaa aggtcctttg
      301 ccctcacaaa acaagggtcg tatgtaggcg ggaacgaaac cgggaaagtc gaaacatctg
      361 cccaaggctg aaggcttaac gcttaatgct tttggcgtta ggctttcggc tttctgctca
      421 aattcctgcg ctcttgcacg gatattattt ccataatcaa aagtgatggc tcccttatcc
      481 atcagttgca gcatcaactg cacatgcagg tgcatactct cgtaactgcg gcgtatatac
      541 tcttccgggt tttcctgtct tattacattg gcctgttcgt tgctgagcgt atgtggtaca
      601 taaccgatca gcgggtcatg ggcactggtc tggtcagtca atgtatcggg aatgatatca
      661 cgttgtatca ggcgatccag caggtcaacg gcattacaca acaccccaat gctccgggct
      721 tccccttcac ggcggtaatg cgtggcagcg ttaatggctt catcaatgct ggtatgtttt
      781 tcatcgaggt attttgtttc gatccgtttg tcaatccgcc attcttccac ttcggcaata
      841 agtgctactc cttcattcat ggtgatggcc aacggctgtg cgccgcccat accaccgaga
      901 ccggccgtta cgttcaacgt accctttaaa ctaccgttga aatgcttgtc ggcaagagct
      961 gcataagttt cataagtacc ctgcactatc ccctgggaac caatgtatat ccaacttccg
     1021 gccgtcatct gcccatacat catcaggccc tttttttcca gctcatcaaa atgtttccag
     1081 ttggcccagt tgggtaccag ctggctgtta ctgataagca ccctgggggc atctttatgt
     1141 gtatttaaaa tacccaccgg tttgccggat tggataagta aagtctcatc gttctctact
     1201 attttgagag cagcgatgat attatccagt gcttcaaaat tgcgggccgc ttttcctctg
     1261 ccaccataca caataagatc atcgggacgt tcggctactt cggggtcaag attgttgagc
     1321 aacatgcgaa gggcagcttc ctgtatccat cctttgcagc tcagttttgt gccggttggg
     1381 gttttatact tgatagcgtc gtagcgtttg gtgagtgtac tcatgctgta ttatgttgaa
     1441 tgttgaatta taaatgttga atgcatacca ggttatatca tttataatac aacattcaac
     1501 atttaacata tgacgctgca aaagtcgtta ttctctttca aattaaatat cttcaccgca
     1561 cattaacctt aaccaatgag tatccacatc agtgcccaaa agggagagat cgccaaaaca
     1621 gtactgctgg ccggggatcc gctgcgggcc aaatatattg ccgataatta tttacaggat
     1681 gtactactgg taagccagac ccgtaatgct ttttactaca ccggcactta caaaggaaaa
     1741 ccggttaccg ttggcgccag tggcatgggc tgtccttcga tcggtattta ttcatacgaa
     1801 ctgttcaccg agtatgaagt ggatacgatc atccgcattg gcacctgcgg cgcttatacc
     1861 actaaactga aattgtttga cctggtgaat gtagattatg ctgccagcga atctactttt
     1921 gcccggtatg catggggcat aaaagaccaa tgcatccgcc accagggaaa agcatttaaa
     1981 accattaaaa aaacagcgac agaattaaat atagccctga agaattgcaa tattcactcc
     2041 agcgatattt tttaccgcaa agacagcaat acacccaaag tggccaccgc caataaatgc
     2101 ctggcagtgg aaatggaggc cttctcatta tttgccaatg cccttcacct gggaaagaca
     2161 gccgctaccc tgctgacagt ttcagatata attcccactc atgaaaaaat aactgcggac
     2221 cagcgggaaa ctgccttgca gccgatgatg gtgctggcgc tggaaacagt ggtgaagttg
     2281 tgagttactt tacattaaaa caaaaattaa cctcttaccg caatcttgtc gtcccgagga
     2341 acgagggacc tcaaacccag cgcaatttgc atacattttg attgagcttt cagtgtcttg
     2401 tcactccgac gcaggagaag tctcaacaag tggccctgct atttagtaaa ttaactttga
     2461 gatgcctcgt tcctcaacat gacaagaccc tgaaaaattg aagacttact tttattctac
     2521 gaaactcaac ttctttttac tacttcctcc ggaggccagc caccacaaac agttggataa
     2581 agaaatgtcc aatcaggatt cattgtttta ataaggtcaa gtttcttttg tcttcgccat
     2641 ccttttattt ccttttccct ggcaatagca ttattgatgt aagtaaaatc ttcgtagtag
     2701 ataagattgt aacaataata tttaccggca aaggttttgg gttgcccccg attggcccag
     2761 tgttccttta gtcgttgcga aagattattg gatacaccag tataaagaac agtacgttca
     2821 ggatttgtga caatgtaaac gtagtaatgg tgttgtttat agataaacat agtatccttt
     2881 cctgttagtc tttgagacgc ctccttcgtc ggcgtaacaa aaattcttac agagctgaga
     2941 actcctgaaa cccactgttc agttcaatgc cttttgaagc agcaacttca ttcacctttt
     3001 ctacaaatga aaaatctttg atgagtccgg caaccttgtt gatatcatct gcaaagatcc
     3061 ggtcctcctc tgcaaaacct acatactccc gtacataatc atgcgcaaat tccaataaca
     3121 ccgaactctt taacggcctt ctgaattcaa tggcctggca ggcagaaagt aattcaatgg
     3181 ccaggatgaa ttccaggtta tctaacacac ggttcagttt tctgccactg atagaaccca
     3241 tactcacatg gtcttcctgc ccgagtgaag tgggaatgct atcagcgctg gccgggaagc
     3301 acaatgtttt attctccgta accagtgcag ccgtagtata ctgtggtatc ataaatccac
     3361 tgttgagtcc cacgtctttc atcagtaaca tcggcaagcc ccatttgcct tccagtaata
     3421 aataacaacg ccggtcagat atatttccga tctcagcagc agcaaagcaa caataatcca
     3481 atggcaaagc catcggctga ccatgaaagt tgccaccact gatcgtatca tcagctccca
     3541 aaataatggg attatcggta acagcattca attctatctc tgtcaattct tttaaatgta
     3601 accaggcatt gcggcttgcc ccatgcacct gcggcatgca acgcagtgag taagggtcct
     3661 gcacccggcc acaatccata tggctttgca taatggctga atcattcagt aacagacgta
     3721 agcgttgtgc cactaattta tttcctttgt aaggacgcaa ttcatgcaat cgttcatcaa
     3781 aaggagcttt ggtaccggta agagcttcca ggctcatcgc cccgatgata tcggccgctt
     3841 ccaaacaatt gtgcattcgt tgtaccgctt tcacggcaaa ggataaaatg aattgggtac
     3901 cgttgatgag tgccaatcct tctttgggcc ctaattgaac aggttgaagt cctttttctt
     3961 ttaatacttc acttgtattt tttcttattg tctcctttcc attcttagcc catacttctc
     4021 ccaggccaat cagtggtaaa aacaaatgcg ccagtggcgc caggtcaccg ctggctccca
     4081 cacttccttt ttccggcacc acgggtatca cattattttc aatatgccaa gtgatccgtt
     4141 ctaaagttga taattgcacc ccgctgaaac cctgtgccag tgcatgcact ttagtgatga
     4201 gcattatttt ggccacttcc accgcaatag ggtcacccac tcccacactg tgactttgca
     4261 atatcttata ctgtaatgtg gccgtgtcct ctttagagat gggagtattg gcgaggatgc
     4321 caaagccggt attgacacca tagaccgttt tgttttccgc tacgatctgc tgcacatatt
     4381 gctggctggc cttaattttt tttattgctt cttcacttaa taccgctttc aatttccccg
     4441 atgcaatagc gatcgttttt tcaacagtca gttgatcggt accgtagcga aatggattac
     4501 tcatagcagg caattaggcc tgcaatttaa acgatttgca gcgaccgttt tataattaat
     4561 aacagcctga ttgcaacctt tgtcaattgg cgtttatctt tatgcaaact tcctttcttt
     4621 atgctacgat tgttctcctt attggcaagc ctgttgatca tgttaactac gggtgcacaa
     4681 agcctgaaaa aatatcctgt cagtaattcc ggatgcacag catacactta ttgtgaaatc
     4741 aaatacgaat tgagcaaaag tgctgactct tcttccattt attccggtga gtgcactaag
     4801 gaagacgtta actatggcat tatatgtgta aagctgctga atcctataac agaacttccg
     4861 ctggcagaag acatgatgat tgcttatgca gattacctga aaacaagttt tgagatcacc
     4921 aaaagcatgg gatacggccg gggccacctg ctgaacaata aagagaatac ccggggcatc
     4981 attgattact gggaagatgg ggaaaagaat aaatggaaga ttaaagcctg gacggatggc
     5041 aagttcatcg gtttcctgta tgtatacagt aaaaaggaat tgccggaagc aaaagtgaat
     5101 gcctttttgg atagttttcg gttgcctggg cagtgagtgt gtaaagaata atgaataaaa
     5161 aaagcgtcac gattatacac cgcgacgctt ttttgtgatc cggattggat tcaaaccaat
     5221 gacctactgc ttagaaggca g
//
LOCUS       DAORTI010000193         5093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_20109_length_5093_cov_6.713180, whole genome shotgun sequence.
ACCESSION   DAORTI010000193 DAORTI010000000
VERSION     DAORTI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20109_length_5093_cov_6.713180"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..788)
                     /locus_tag="PLO70_15645"
     CDS             complement(135..788)
                     /locus_tag="PLO70_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75954.1"
                     /translation="MQKLLIAAATLLMLSFVSCKSSTTTPKTFCDTVCLTDTIKFTGT
                     HEKTPTVFITAKNCLPDSIIWSYKGMAADRKTGFTYLMGTTVNINKNFIRCFFKDNEY
                     AWLLFNDCMTGRGFQIKLPYDKNANFSLKSSGINSLDPKFSIAENLVVNTDRGNIYVE
                     DMSSGKKAMMTFGEPLPIDYDALHDHIDSVNVTNSRIWVKILVGKNWVEKEKNIVLE"
     gene            892..1401
                     /locus_tag="PLO70_15650"
     CDS             892..1401
                     /locus_tag="PLO70_15650"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQZ75955.1"
                     /translation="MSNMQPINLRSFKQKARYQKTGMRRFLSKLDKQAPRGLDKKIAG
                     LEKEVWAETDCLSCANCCKTMTPTFTKQDLKRISAHFNMTVDAFQKKWLRKERGGDRD
                     WLNKTEPCQFLNLKDNKCSIYEIRPADCAGFPHLSKKFKDFVHIHKQNVEYCPATYRL
                     VEKMMMGVD"
     gene            complement(1461..1817)
                     /locus_tag="PLO70_15655"
     CDS             complement(1461..1817)
                     /locus_tag="PLO70_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQZ75956.1"
                     /translation="MASHNELGKEGEEMAAKWLTEKGYTILHRNWRHSYHELDIVASK
                     GKFLHFVEVKARKASPFGNPEDSVTKKKFKNLQRAADEYLFLHPGHSWIQYDILSITI
                     HQNGEAEYFLIEDVFL"
     gene            1856..3025
                     /gene="manA"
                     /locus_tag="PLO70_15660"
     CDS             1856..3025
                     /gene="manA"
                     /locus_tag="PLO70_15660"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HQZ75957.1"
                     /translation="MPGIYRLKGVVKHYDWGGYSFIPALLNIENKYHQPFAEYWMGIH
                     PLGSALVETGGADPTALSALVEKLSFLFKVLDVKNMLSIQVHPSKEAAAIEFARENTE
                     AIPLDSPKRNYKDDNHKPELMVALSDFWLLHGFKPPEELGNTLLNVVELRELLPVFNQ
                     TGYAGLYKFVMEMPQEEVNRILQPLIDNISAIYKDKKPDKTDEDFWAARAAQTFSNDG
                     NIDRGIFSIYFFNLVHLKKGEGIYQPAGVPHAYLEGQNVEIMANSDNVLRGGLTTKNI
                     DVKELLKHVKCEATFPTILPGERTKEEKTYKTPAPDFQLSVFELEKGDTISFQPEEVE
                     ILLLTEGVVELDDDKETIKLQKGDPAAVVFPGQVVYLAAAAKSTVFRASEAFHNR"
     gene            3097..3666
                     /locus_tag="PLO70_15665"
     CDS             3097..3666
                     /locus_tag="PLO70_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75958.1"
                     /translation="MKSNGRLIVFTLILVALATACKYFFGPDIGWSGFSPVIAIALFA
                     GFIMKQKDMSFILPLLALFISDAVIQFLYSQDLFPYAGFYGGQWKNYAVLLAATLIGW
                     VLKGRSFSSLMAGALAAPTVFFLVSNFMVWAASTEAVYAKSFSGLMVCYEAGLPFYRN
                     SLIATLVFLPVILVAYNYLTKKKAVLTLA"
     gene            complement(3798..4268)
                     /gene="rnhA"
                     /locus_tag="PLO70_15670"
     CDS             complement(3798..4268)
                     /gene="rnhA"
                     /locus_tag="PLO70_15670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQZ75959.1"
                     /translation="MNTVTIYTDGSARGNPGPGGYGVVLMSGHHRKELSQGYKHTTNN
                     RMELMAVIAGLKALKKEGMQVTIYSDSQYVVKAWEEGWLKNWIKTNFKGGKKNPDLWW
                     EFYELSQKHHIKFRWVKGHADNPYNNRCDELATEAADGHHHKLLHDEGYEGTAS"
     gene            complement(4336..>5093)
                     /locus_tag="PLO70_15675"
     CDS             complement(4336..>5093)
                     /locus_tag="PLO70_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQZ75960.1"
                     /translation="PYNMVDVIERIVDDSEFEQFKEDYGKSIVCGYGRIDGWAVGIVA
                     NQRLIVKNKKGEMQMGGVIYNDSADKAARFILNCNQKKIPLVFLQDVTGFMVGSRSEH
                     AGIIKDGAKMVNAVANSVVPKITIIIGNSYGAGNYAMCGKAYDPRFIYAWPTAKIAVM
                     GGDAAAKTLLQIEVASRKAKGETLNAEVEQKMFSEIKNRYEKQTSPYYAAARLWVDTI
                     IDPVDTRKVISEGINAANNNPEMNDLKTGVFQV"
BASE COUNT         1396 a         1044 c         1051 g         1602 t
ORIGIN      
        1 gtaagctttg aatccttttt tgtagggctc ccacatggag gttaattggt ttattttttt
       61 atagttaatt agggaaacct ttttagagca gattcttcct gattaaccaa ctcccaatta
      121 actaattaac tattttattc cagcacaata ttcttctctt tctccaccca atttttacca
      181 acaaggattt ttacccatat ccttgagtta gtaacattaa cagagtcaat atggtcgtgc
      241 aatgcatcgt agtcgatggg cagtggttcc ccaaaggtca tcattgcttt tttgccgctg
      301 ctcatgtctt ccacatagat atttccccgg tcagtattga ctaccaggtt ttcagcaatg
      361 gaaaatttag gatcaagact attgatgccg ctgctcttca ggctgaagtt agcattctta
      421 tcgtacggta atttgatctg gaaaccacgg ccggtcatgc aatcgttaaa cagtaaccag
      481 gcatattcgt tgtctttgaa aaaacaacgg ataaaatttt tgttgatgtt aacggtagtg
      541 cccattaagt aagtaaaacc tgttttacga tcggctgcca tgcctttata actccagatt
      601 atgctgtcag gtaaacaatt cttagcagtg atgaatacag ttggcgtttt ctcgtgggtg
      661 ccggtaaatt ttatcgtatc agtcaggcaa accgtatcac aaaatgtttt cggtgtggtg
      721 gtggatgatt tgcaggaaac aaaagaaagc atcagtaagg tagcggcagc aattaggagt
      781 ttttgcataa taattggttt aacagactgc aaaaataacc attgcaaaac tctgatgtaa
      841 aaattctttt gatgagtgaa ctgaaactgc ctaatcatta tcttcgggct tatgagtaat
      901 atgcaaccca ttaatctgcg ttcgttcaaa caaaaagccc gttatcagaa aacgggtatg
      961 cgccgtttcc tttcgaaatt ggacaagcag gccccccggg gactggataa aaaaattgca
     1021 gggctggaaa aagaagtttg ggcagaaact gactgtcttt cctgtgctaa ttgttgtaag
     1081 acgatgacgc cgacatttac caaacaggat ttgaaaagaa tatcggctca tttcaatatg
     1141 actgtggatg cattccagaa aaaatggctg cgtaaagaaa ggggtggtga ccgtgactgg
     1201 ctcaataaaa cagaaccctg ccagttcctg aatctgaaag ataataagtg cagtatttat
     1261 gagatcaggc ctgctgattg tgcaggcttt ccgcatttat ctaaaaagtt caaagacttt
     1321 gtgcatattc ataagcagaa tgtagaatat tgcccggcaa cgtaccggct ggttgaaaag
     1381 atgatgatgg gtgtagacta gtactatgtt caggattggc attttaaccc aggttccagg
     1441 ctagctgaat attatgcacc ttaaagaaag acatcttcaa ttaaaaaata ttcagcttcc
     1501 ccattttgat ggatggtgat tgaaagaata tcatattgta tccatgaatg accgggatga
     1561 agaaaaagat attcatcggc tgctctttgc aggttcttga atttcttttt ggtgacacta
     1621 tcttccgggt taccgaatgg tgatgccttt cttgctttta cttccacgaa atgtaagaac
     1681 tttcctttgc tggctacaat atccagttca tgataagaat gccgccagtt gcggtgcagt
     1741 atagtatacc ctttttctgt cagccatttt gctgccatct cttccccttc ttttcccagt
     1801 tcattatgtg aggccatgta ctatctttac gacaagataa aaaagaaaat tatttatgcc
     1861 gggcatttat cggttgaaag gggtagttaa acattatgat tggggtggat actcctttat
     1921 tcctgcctta cttaatatag agaataagta tcatcagcca tttgctgaat actggatggg
     1981 tatacatccc ttgggcagtg cgttggtgga aaccggtggt gctgacccca ccgcactctc
     2041 tgctctagtt gaaaagcttt cttttctttt caaagtgctg gatgtaaaaa atatgttgtc
     2101 catacaggtg catccttcca aagaagcagc agcaattgaa tttgcccggg aaaatacaga
     2161 agctattccg ctggattcac caaagcgtaa ttataaagac gacaatcata aaccggaatt
     2221 gatggtggct ctcagcgatt tctggttgct ccatggtttt aaaccaccag aagaattagg
     2281 taatacttta ttgaatgtgg ttgaattaag agagttgctg ccggtcttta atcaaacagg
     2341 ttatgccggg ctctataaat tcgtaatgga aatgccgcag gaagaagtta accgtatatt
     2401 gcagcctttg attgacaata tatctgcaat ttataaagat aaaaagccgg ataaaacaga
     2461 cgaagatttt tgggcagcca gggctgcaca aactttttct aatgatggca atattgaccg
     2521 gggtattttc tctatttatt tttttaatct tgttcacctg aaaaagggag agggtatcta
     2581 ccagcctgcc ggtgtaccgc atgcctacct ggaagggcaa aatgtggaga ttatggcgaa
     2641 ctctgacaat gtgctccgtg gcgggctcac caccaagaat attgatgtaa aagaattatt
     2701 gaagcatgta aaatgtgagg ccactttccc aaccatactt cccggagaga ggaccaaaga
     2761 ggaaaaaact tataaaactc cggccccgga ttttcagtta agtgtttttg aactggaaaa
     2821 aggggatacc atttcttttc aacctgaaga agtggaaata cttttactca cggaaggagt
     2881 ggtggaactg gatgatgaca aggagactat taaattgcag aagggtgatc cagcagctgt
     2941 agtattcccg ggacaggtcg tttacctggc agccgccgcc aaatccactg ttttcagggc
     3001 ttccgaggct ttccacaatc gttaataaat agcccgtcta attggggcaa ttctgctaat
     3061 tttgctccgt atttcaagaa cttataataa aatgttatga agagcaacgg acggttaatt
     3121 gtttttacat taatactggt agcattagca acggcctgta aatatttctt cggacctgat
     3181 atcgggtggt ccggtttctc tccggttatc gcaattgctt tatttgccgg atttatcatg
     3241 aaacaaaaag atatgtcatt tatcctgcca ttgctggctt tattcatcag tgatgctgtg
     3301 atccagtttt tatatagcca ggacctgttt ccttatgcag gattttatgg aggtcagtgg
     3361 aagaattatg ctgtcttatt agcagccaca ctgatcggct gggtattaaa aggcagaagt
     3421 ttcagcagcc tgatggccgg agccctcgct gctcctacgg tattcttttt ggtctctaat
     3481 tttatggtgt gggcggcttc tactgaagca gtgtatgcaa aaagtttcag cggtttgatg
     3541 gtttgctatg aagccggact tccgttctac agaaattcct tgatagcgac tttagtgttc
     3601 ctgccggtga tcttggtggc gtataattac ctgacgaaga agaaagcggt acttacgtta
     3661 gcataagatt ttaataaaaa aaatctgaac ccatcagtat ttgctggtgg gttttttatt
     3721 agcggagtat tcagctcttc taattaaaga aaaagaaaca ggttctttca tttttggagc
     3781 attgttggag taagaattta actggcagtt ccttcatatc cctcatcatg cagcaattta
     3841 tgatggtgcc catctgccgc ttcagtagct aattcatcac aacggttatt atacgggtta
     3901 tcggcgtgac ccttcaccca tctgaatttg atatgatgtt tttgagagag ttcatagaac
     3961 tcccaccaca ggtccgggtt tttcttgccg cctttgaagt tggtctttat ccagtttttc
     4021 agccagcctt cttcccaggc tttcaccaca tattgactgt cggaataaat agtgacctgc
     4081 attccttctt ttttcaatgc tttcaacccg gcgatcaccg ccattaattc catcctgttg
     4141 ttagtggtgt gtttatatcc ctgtgacaat tccttgcggt gatgaccgct cattaatacc
     4201 acgccatatc caccgggacc gggattgcct cttgcagaac cgtctgtata gattgttact
     4261 gtattcatta attatgctga atgttgaatg ataaatgttg aattaatagt tttctattca
     4321 gcattcatca taaaattaaa cctggaatac tccggtcttt aaatcattca tttcaggatt
     4381 attattggcc gcattaattc cttccgaaat aacctttcta gtatccaccg ggtcaattat
     4441 agtatctacc cataaccggg ctgctgcata ataaggggag gtttgtttct cataccggtt
     4501 ttttatttcg ctgaacattt tttgttcaac ttctgcattt agtgtttcgc ctttagcttt
     4561 acgggaagcc acttctattt gtaataaagt ttttgctgcc gcatcaccac ccatcactgc
     4621 gatctttgct gtgggccatg cataaataaa acggggatca tacgctttac cacacatggc
     4681 atagttaccg gccccgtaag agttaccaat gatgatcgta atttttggta ccactgaatt
     4741 cgctactgca tttaccatct tcgccccgtc tttgataatg cccgcatgtt cactccggct
     4801 gcccaccata aagccggtca catcctgcag gaataccaat ggaattttct tctgattaca
     4861 gtttagaata aatcttgctg ctttatcggc actgtcatta tagatcacac cgcccatctg
     4921 catctcgcct ttcttattct ttacgatcaa tcgctgatta gctacaatgc ctacggccca
     4981 accgtcaatc cggccataac cgcaaactat ggatttacca taatcttctt tgaactgctc
     5041 aaattcactg tcatcaacta ttctttctat cacatccacc atattatatg gtt
//
LOCUS       DAORTI010000194         5084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_20167_length_5084_cov_6.374627, whole genome shotgun sequence.
ACCESSION   DAORTI010000194 DAORTI010000000
VERSION     DAORTI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20167_length_5084_cov_6.374627"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLO70_15680"
     CDS             complement(<1..221)
                     /locus_tag="PLO70_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75961.1"
                     /translation="MKAMKSTLMLLSLFMYGLSSAQEFDRTASVGFFNGVINYQGDLN
                     PNSFTLKHSNFATGIFARKPMNRWLTLRAG"
     gene            complement(362..1771)
                     /locus_tag="PLO70_15685"
     CDS             complement(362..1771)
                     /locus_tag="PLO70_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQZ75962.1"
                     /translation="MTTQLIKKVLVGILFTVALSGLYAQQVKKAKEGVKKANIGVSFN
                     MHDFDTESLFPKEDSVYGFSVMFWKGISPKVDYSIRYNGIFPNKNVNDVISKNALSSE
                     LEAAIHAKAFPDKTILNPFLTLGVGGGSYHKDLTAYANANNNSELNAHALGGVGLQLN
                     IKSELYFLAQAHYRYSFNENRLPHNMFYSIGLTKSLSKKEAAKPVVADRDKDGVPDNA
                     DACPDVAGIASLQGCPDKDGDGIADKDDACPDVAGLKELNGCPDRDGDGIADKDDKCP
                     DTKGLAKYNGCPIPDSDGDGVNDEEDKCPSVAGVQRYQGCPVPDADNDGVNDEEDKCP
                     NLAGVRENQGCPVISEEITKKVGMAAKNILFVTGSAKLQKSSYKGLNDVVKILQDNPD
                     MKLSIEGHTDNVGSDDKNQVLSENRANTVKDYVVSKGIDASRITSSGFGETKPLADNK
                     TAAGRQQNRRSELILSYYQ"
     gene            complement(1873..2907)
                     /locus_tag="PLO70_15690"
     CDS             complement(1873..2907)
                     /locus_tag="PLO70_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQZ75963.1"
                     /translation="MKVHYWQYNLKFRHPFTISKGTKTHQPTLIVELEHMGIKGYGEA
                     PAIAYYNIPVDKMIADLESKKSLVEKFAFTDPERYWHYLHHLFPQNNFLVCALDIAAW
                     DIYGKMKGRQLYELWKGDVSKNPICDYTIGIDTIDKMIAKMKEKPWPVYKIKVGTADD
                     IAIVKALRENTDATLRVDANAAWDLETALQLIPQLKELGVELVEQPLAKEDWEGMKVL
                     YKESSLPLYADESCVVEQDVERCKDHFHGINIKLTKCSGITPARRMIQKARELSMHVM
                     IGCMNESTVGAAAIAHLLPFIDHVDMDGPLLLEEDLATGIGYNYGKISYSGNPGLGIT
                     YTGLFSKKLD"
     gene            complement(2923..>5084)
                     /locus_tag="PLO70_15695"
     CDS             complement(2923..>5084)
                     /locus_tag="PLO70_15695"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQZ75964.1"
                     /translation="KLDLSDSDFPVAYKYGVFNTSEKSFTLYEDGDNRLLYSDIVKKK
                     LIIVHDGFVHLPNNTWKGAGVAIPVFSLRSKNSFGVGEFTDIKLLADWSKATGLKLIQ
                     LLPVNDTIATNSWMDSYPYAAISAFALHPLYINLSKVAGREYSGQLRSLKKKQKQLNE
                     LAEVDYETVINYKLSVLKELYEVMGKECFETEDYKDFFKDNKHWLQPYAAFCYFRDKY
                     GTSHFGDWKTNSTYNKAAIEKLFVATSSIAKEIGLFCFIQYHLHLQLKDAADYAHKKG
                     IVLKGDIPIGIYRNGCDAWVAPELYNMEWQAGAPPDDFTAVGQNWGFPTYNWKRMQED
                     DFSWWKKRFEQMSNYFDAFRIDHILGFFRIWSIPADAVQGIMGRFVPCLPVHINEFGE
                     NGIWFDYQRYCRPFITDEILHEVFGEQATPVKEKFMVPNDFGSYDLAPEFETQVQIEK
                     YFSGLEESVENEKLKLQLFDIISNVILFEEPGSQGQEFHFRISMEKTLSFRNLIPHVQ
                     EKLKDLYVNYFYRRQDDFWFKEAMHKLPQLKMATNMLVCGEDLGMVPDCVPDVMQQLG
                     ILSLEIQRMPKDPKKEFFHPSDAPYMSVITPSTHDMSTIRGWWEENRERTQQFYNHGL
                     GQWGDAPFYCEAWINRAIVLQHLYSPAMWSIFQLQDILGMSETLRREKPQEERINNPA
                     NPKNYWQYRMHISLEDLIKQTEFNEELNGYIVHSGRG"
BASE COUNT         1385 a         1140 c          936 g         1623 t
ORIGIN      
        1 cctgctctca gggtaagcca gcgattcatg ggtttacggg caaaaatgcc tgtagcaaaa
       61 tttgaatgct tcagggtgaa actattggga ttcaggtctc cctggtaatt tattaccccg
      121 ttaaaaaaac caactgatgc tgttctatca aattcctgtg cacttgataa gccatacata
      181 aacaggctta acagcatgag ggtggatttc attgctttca cagataatgg atttatagct
      241 tgttctgcac aagccatttc aataacggtc agcagaccgg aatggtataa tgagagacaa
      301 ttttattaaa aaaaacaggc tgtttttggc agcctgttta aataattctc tgtttccgga
      361 cttattgata atagctcagg atcaattcag agcggcggtt ttgttgacgg cctgctgctg
      421 ttttattgtc tgctaaaggt ttggtttcac caaatccgct ggaagtaatg cgactggcat
      481 caatcccttt actgacgaca taatctttca ccgtattggc acggttttca ctcaatacct
      541 ggtttttatc atcacttcct acattgtctg tatgtccttc aattgacagt ttcatatccg
      601 ggttatcctg caatatcttc acaacatcgt tcaatccttt ataggaactt ttctgcagtt
      661 tggcacttcc tgtaacgaac aatatatttt tcgcagccat gcctactttc tttgtgatct
      721 cctctgatat gaccgggcaa ccctggtttt cccttacgcc ggcaagattt gggcatttat
      781 cttcttcatc atttacgcca tcattatctg catcgggaac aggacaaccc tggtaacgtt
      841 gtacaccagc tacagaagga catttatctt cttcatcatt cactccatca ccgtctgaat
      901 caggtattgg acaaccattg tatttggcaa gcccttttgt atccgggcat ttatcatctt
      961 tatctgcaat gccatctccg tcacgatcag gacaaccatt caattccttc aaaccggcaa
     1021 catcgggaca tgcatcgtct ttatcggcaa taccatcgcc atctttatcg ggacacccct
     1081 gcagggatgc tataccagcc acgtccgggc aggcatcagc attatcagga acaccatctt
     1141 tatcacggtc ggctacaaca ggtttggccg cttctttttt agaaagtgat tttgtcaaac
     1201 caatggagta aaacatatta tgtggcagcc tgttttcatt gaagctatag cggtaatggg
     1261 cctgtgccag gaaatataat tcacttttaa tatttaactg caaacccacc ccacctaatg
     1321 catgcgcatt taattcacta ttattatttg cattcgcata ggctgtaagg tctttatgat
     1381 aactaccccc gcctacacct aatgtaagga aaggattaag aatagtttta tccggaaatg
     1441 ctttagcatg aatggccgct tccagttcac tgctcagtgc attttttgat ataacatcat
     1501 tcacattctt attggggaat attccattat agcggattga ataatccact tttgggctta
     1561 tccctttcca gaacatcact gaaaatccgt aaactgaatc ttccttgggg aataaagact
     1621 ccgtatcaaa atcatgcata ttaaaactga ccccgatatt tgctttttta accccttctt
     1681 ttgctttttt aacctgttga gcataaaggc ctgaaagtgc aacggtgaat aaaataccaa
     1741 ccagcacttt ctttattagt tgcgttgtca taatgggtaa aattttgtac aaaattactc
     1801 ttccgtcgat ggaaagacta tgatttttat caaacaggtg agtctgaaaa agagattaaa
     1861 atcatacaaa ttttaatcca actttttgga gaataagcca gtataggtaa tgccaaggcc
     1921 cggattaccg gaataggaaa ttttaccgta attgtaccca ataccggttg ccaggtcctc
     1981 ttcaagcagg agcggtccat ccatatccac atgatctata aagggaagta aatgtgcgat
     2041 agctgcagca cccacagttg actcattcat acagccgatc attacatgca tactcaactc
     2101 tcttgctttt tgtatcatcc ggcgggctgg tgtaataccg ctgcatttgg tcaatttgat
     2161 attgatgcca tgaaaatgat ctttacaacg ttctacatcc tgttcaacta cacaggattc
     2221 atcggcatat agtggcagtg aagattcttt gtacaaaact ttcattcctt cccaatcctc
     2281 ttttgcgagg ggctgttcca ccagttcaac tcccagttct tttaattgcg gaattaattg
     2341 taaagctgtc tcaagatccc acgctgcatt tgcatctacc cttaaagtgg catctgtatt
     2401 ttcccgtaat gcttttacaa ttgcaatatc atcagctgtg ccaactttaa ttttataaac
     2461 aggccagggt ttctctttca tcttcgcaat cattttatca atagtgtcta tgcctatagt
     2521 gtaatcgcat attggatttt ttgaaacatc acctttccag agttcatata attgcctgcc
     2581 cttcatcttt ccgtatatgt cccaggcagc tatgtccagg gcacaaacaa gaaaattatt
     2641 ttgaggaaac aggtgatgca ggtaatgcca gtatcgttcc ggatccgtaa aagcaaattt
     2701 ctccactaac gattttttgc tttcaagatc tgcaatcatt ttgtctaccg ggatattata
     2761 ataggcaata gccggggctt caccatagcc ctttattccc atatgctcca actcaacaat
     2821 tagggttggc tgatgggtct tggttccttt ggaaatagtg aaaggatgtc tgaatttcag
     2881 gttgtattgc cagtaatgaa ctttcatggc agcaaatata gtttatcccc tgccgctatg
     2941 tactatatag ccattcagct cctcattgaa ttctgtttgt tttatcagat cttccaatga
     3001 aatgtgcatc ctgtattgcc agtagttttt tggattggca ggattattta tcctttcttc
     3061 ctgtggcttt tccctccgca aagtttcact catgcccaat atatcctgta actggaagat
     3121 actccacatc gcaggtgagt ataggtgttg taatacgata gccctgttga tccatgcttc
     3181 gcaataaaaa ggtgcatcac cccattgtcc caatccatgg ttatagaatt gttgggttct
     3241 ctcacggttt tcttcccacc atccgcggat ggtgctcata tcgtgtgtgg aaggtgtgat
     3301 cacggacatg tatggggcat cactaggatg gaaaaattct tttttgggat ccttgggcat
     3361 tctttgtatt tccaggctta gtatacccaa ttgctgcatt acatccggca cacaatccgg
     3421 aaccatacca aggtcttctc cgcaaacaag catattggtg gccatcttca attgcggcag
     3481 tttatgcatg gcttctttaa accaaaaatc atcctgtctc cggtaaaaat aatttacata
     3541 caggtctttc agcttttcct gcacatgtgg tataaggttc ctgaatgaaa gtgttttttc
     3601 catcgagatc ctgaaatgga attcctggcc ctgtgagccg ggttcttcaa ataagatcac
     3661 attagaaata atatcaaaca gctgcaattt cagtttttca ttttctactg attcttcaag
     3721 acctgaaaaa tatttttcta tttgtacctg ggtttcaaac tcaggtgcca ggtcataact
     3781 gccaaaatca tttggcacca tgaatttttc tttcacaggg gttgcctgtt caccaaagac
     3841 ttcgtgcagt atttcgtcgg tgataaaagg gcggcaatat cgctggtaat cgaaccagat
     3901 cccgttttct ccgaactcgt taatatgtac tggcaggcat ggtacaaacc ttcccattat
     3961 tccctgcact gcatcagcag ggatactcca gatgcggaag aaaccaagta tatgatctat
     4021 ccggaatgca tcgaagtaat tgctcatctg ctcaaatcgt tttttccacc aggaaaaatc
     4081 atcttcctgc atccgcttcc agttgtatgt gggaaaaccc cagttttgtc caaccgcagt
     4141 aaaatcatcg ggtggtgccc cggcttgcca ttccatgtta tataactcag gagccaccca
     4201 ggcatcacag ccgttgcggt atattccaat ggggatatca cctttcagca caattccttt
     4261 tttatgggca taatctgcag cgtccttaag ctgaagatgc aaatgatatt gaataaagca
     4321 aaacaatcct atttctttcg ctattgaaga agtggcgaca aataattttt caatagcagc
     4381 tttattgtaa gtgctattgg ttttccaatc accgaaatga gatgtaccat atttatccct
     4441 gaaatagcag aatgctgcat aaggctgtaa ccagtgttta ttatccttga aaaaatcttt
     4501 ataatcttca gtctcgaaac actcttttcc catcacttca tacaattctt tcaatacaga
     4561 aagcttgtaa ttaattacag tctcataatc tacttcagcc aactcgttca gttgtttctg
     4621 tttcttcttt aaagaacgta actggccgct atattcccta ccagccactt tggacaggtt
     4681 aatatatagc gggtgtaatg caaatgctga gatggctgca tagggataag agtccatcca
     4741 gctattggta gcaattgtat cattgaccgg aagaagctga attaatttca ggccggttgc
     4801 ttttgaccag tcagccagca attttatatc tgtgaattcc cctactccaa agctgttttt
     4861 acttcttaga ctgaaaacag ggatcgccac tccggcacct ttccatgtgt tattaggcaa
     4921 atgaacaaag ccatcatgaa caataattaa tttctttttt acaatgtctg aatataagag
     4981 cctgttatcc ccatcttcgt ataaagtaaa cgatttctca ctcgtgttaa atactccgta
     5041 tttataggct actggaaaat cgctgtcgga caaatccaat ttta
//
LOCUS       DAORTI010000195         5040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_20452_length_5040_cov_5.744835, whole genome shotgun sequence.
ACCESSION   DAORTI010000195 DAORTI010000000
VERSION     DAORTI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20452_length_5040_cov_5.744835"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLO70_15700"
     CDS             <1..778
                     /locus_tag="PLO70_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75965.1"
                     /translation="TESVLFDAPGMNGSPAEFIDYLKAMLMQFKYFNSFTSREIHYLC
                     KDSTIWNFYLFPEMAAFKTFFWSKTINNQASLSNKTFSMEEFPYHDCFGLGQLVLQEY
                     NQIPCVELWNLESMHSTINQVAYYRDAGNFKTNKDFELVLESFHKMIDHLQLQAEKGV
                     KFMPGAAEVSYKAPIRFYINELILGNNTMVINLDGKDLTMVTYSVFHYLFTKDPRFAA
                     KVKTSFDTLLSRSTLISTTGEKDRNRFFNAMREKVNGLRK"
     gene            809..1669
                     /locus_tag="PLO70_15705"
     CDS             809..1669
                     /locus_tag="PLO70_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQZ75966.1"
                     /translation="MNLSSVKLIECPRDAMQGWKTFIPTEKKIEYINSLLKVGFDTID
                     FGSFVSPKAIPQMSDTKEVVKKLGIGVRGTKLLAIIANQRGAEEASIFDEISYLGFPF
                     SVSETFQQRNTNSTIKESLGRVEEIQNICFKTGKQLVVYISMGFGNPYEDPFDEDIVF
                     EWVNKLVAMDIGIISLADTVGVATPEQVYTMTSYLVDSLPGTEIGVHLHSTPQNWKEK
                     LEAAVKAGCKRFDGALKGIGGCPMADDELVGNMNSELMINYFDQHGLVNSLDKEALAH
                     SLRIAGEIFI"
     gene            1676..2677
                     /locus_tag="PLO70_15710"
     CDS             1676..2677
                     /locus_tag="PLO70_15710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HQZ75967.1"
                     /translation="MDLLINIDLKKLPEPISYRDNIMLIGSCFTEHIGNALGEFKFSV
                     LQNPNGILFGPDSVCKSLYSYISPREYLEADLFRLNEIWNSWQHHSRYSNIDSRACIG
                     AINDSQNTAHTFLKKADWLIITLGSSYSYQLTKEADLASESIKNGVANCHRAPANWFT
                     KEMLSIDVISFMLEDCIKQLKQFNPDLKFLFTVSPVRHIRDGVVENNRSKARLIESVH
                     YIVNKFESAFYFPAYELVIDVLRDYRFYDIDLVHPNYPATEFVLEKFAATCINVESQQ
                     LMKEIRKIMIARKHKAFQPATKSHQQFLMSYFEKTRALKDKYPFLQLDEELVYFSQT"
     gene            complement(2668..3720)
                     /locus_tag="PLO70_15715"
     CDS             complement(2668..3720)
                     /locus_tag="PLO70_15715"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQZ75968.1"
                     /translation="MPINQQFCFSHSSGQEVSLFTLSNSKGTKVLITNLGATITSFKL
                     KNADGSVNDIVLGFEKIEDYAGADYLAQYPWFGCAVGRVANRIKNAEFMIDDKNYSVS
                     RNNGNNQLHGGLEGFDKKVWQKKAMGDSPDCFLELKYVSPDGEEGYPSNLEVIIRFEL
                     NDNDELSYQYTATCDKTTIVNLTHHGYFNLNNGEGDIKDHEVKIYGSLTLDQDEELVA
                     NGSLTPVENTLFNFSDFVRVGERLTKIAEYDKSYIANKKKEETSLSLMAEVKSAASKL
                     LLQVYSTEPIVHFYTGKWIPTVKGKNGTSYGAFSGLCLETHKHPNAINIPHFPTTILR
                     PGETYSTRTIYKVIQV"
     gene            complement(3749..4816)
                     /locus_tag="PLO70_15720"
     CDS             complement(3749..4816)
                     /locus_tag="PLO70_15720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQZ75969.1"
                     /translation="MNQGSYKEALKAFADHSHEFTVEPVTNGLINQSFKITGNDTGSS
                     FLLQQINHHVFTRPEKLQQNYMLLWKYLREEDIPFIIPEPKHFINDKTFFRDGQNNYW
                     RVFEFISGTKTLNIADNISQAKGVANIFAGFTACFSKFDSNQLHITIPDFHNLSLRFR
                     QFTQSLSNGLNERIRKSQQLIDDLNLRERYVNFYETIIESDEFRLRVMHHDAKISNVL
                     FDTASEKLICPVDFDTVMPGYFFSDLGDMIRSMACSENENSNRYEELNIRKDFYDAIV
                     SGYTEVLNGQLTFSEKKYIHLSGLLMIYMQALRFLTDYLNGDIYYRISYPEHNFDRAT
                     NQVILLKNLEEFLIHNYHFKF"
     gene            complement(4886..5038)
                     /gene="miaE"
                     /locus_tag="PLO70_15725"
     CDS             complement(4886..5038)
                     /gene="miaE"
                     /locus_tag="PLO70_15725"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HQZ75970.1"
                     /translation="MESEAGHYTLFIELAETYISKDKVRRRWKEWLDYEADIIHNLEV
                     RGDRIH"
BASE COUNT         1538 a          965 c          973 g         1564 t
ORIGIN      
        1 aactgagtca gtactttttg atgcccccgg aatgaatgga tccccggctg agtttattga
       61 ttacctgaaa gcgatgctga tgcaattcaa gtactttaat tcttttacat caagagagat
      121 acattactta tgtaaggatt ccaccatctg gaatttttac ttgtttcctg aaatggccgc
      181 attcaaaact tttttctggt caaaaacaat taataaccag gcaagcttaa gtaataaaac
      241 attctccatg gaggagtttc cgtatcatga ttgttttggt cttggtcaac tggtattgca
      301 ggagtataac caaataccct gcgtggagct ttggaacctg gaaagtatgc acagtacgat
      361 taaccaggtt gcttattacc gggatgcagg taactttaaa acaaataaag actttgagct
      421 ggtgcttgaa tcctttcata aaatgataga ccatctgcag ttacaagccg aaaaaggcgt
      481 aaagtttatg cccggagctg ctgaagtctc ctataaagct ccgatacggt tttatatcaa
      541 tgaactgata cttggaaata atactatggt aattaacctt gatggaaagg accttacgat
      601 ggtaacatac agtgtatttc attatttgtt tacaaaagat ccaagatttg ctgctaaagt
      661 aaaaacctct ttcgatacac ttttaagccg ctccactctt attagtacaa ccggagaaaa
      721 ggacaggaac cgttttttta atgcaatgcg ggaaaaggtt aatggtttgc gaaaatagat
      781 tcatagttca attgtatctt tattctttat gaatctatct tcagttaaac ttattgagtg
      841 tccaagagac gctatgcagg gttggaagac ttttattcca acggagaaaa aaattgagta
      901 tatcaattcg ttattaaaag tcgggtttga tacgattgac ttcgggagtt ttgtttcgcc
      961 gaaagcaatc ccgcaaatgt cagacacaaa ggaagtagtg aaaaaactcg ggattggagt
     1021 ccggggcaca aaattactgg ccataatagc taaccagaga ggggcagaag aggcaagcat
     1081 attcgatgaa atttcatatc tcgggtttcc cttctccgtt tctgaaacct ttcagcaaag
     1141 aaatacaaat tcaacaatta aagaatccct gggcagggta gaggagatac agaatatctg
     1201 ttttaaaacc ggtaagcaat tggtggtcta tatttcaatg ggttttggca atccttacga
     1261 ggacccgttt gatgaggata ttgtttttga atgggtaaac aagttagtgg caatggatat
     1321 tggtatcatc tcgctggcag ataccgttgg agttgctacc cccgagcagg tctatacaat
     1381 gacaagctat cttgttgact cattgccggg aacagaaatt ggtgtacacc ttcattccac
     1441 tccccaaaac tggaaagaaa aattagaggc agcagtaaaa gcaggatgca aaaggttcga
     1501 tggtgcatta aaaggaattg gtggctgccc aatggcagat gatgaactgg taggaaatat
     1561 gaacagtgaa ctgatgatca attattttga tcagcatggt cttgtcaaca gtcttgataa
     1621 agaagccctg gcacatagcc tcaggattgc aggagaaata tttatttagt gatttatgga
     1681 tttattgatc aatattgatt taaagaaact tccggaacca atttcttacc gggacaatat
     1741 aatgctgatc ggttcctgtt tcaccgaaca tatcggaaat gccctgggag aattcaaatt
     1801 ttctgtgctt caaaacccca acgggatact ttttggccct gacagtgttt gtaaaagttt
     1861 atattcctac atcagccccc gggaatattt ggaagctgat ttattccgtc tgaacgaaat
     1921 atggaatagc tggcagcacc attcccgcta ttctaatatt gattcaaggg catgtatcgg
     1981 ggctatcaac gattctcaaa atacagctca tacattcctg aagaaagcag actggttaat
     2041 tataacattg ggctcctcct attcttatca acttacaaaa gaggcagacc tcgcatcgga
     2101 gagcataaaa aatggcgtag ccaattgcca ccgggctcct gcgaactggt ttacaaaaga
     2161 aatgctgtca atcgatgtta tcagttttat gctggaagat tgtatcaaac agctgaaaca
     2221 attcaacccg gacttaaaat tcctttttac cgttagcccg gttcggcata taagggatgg
     2281 agtagtggag aataaccgga gtaaggcaag attgattgaa tcagtacatt atattgtcaa
     2341 taaatttgaa tcggctttct atttcccggc ctatgaactg gtgattgatg tgttgcggga
     2401 ttaccgtttt tatgacattg acctggttca tcctaattat ccggccacag aatttgtatt
     2461 agagaaattt gctgctacct gtatcaatgt agagtcgcag caactgatga aggaaatcag
     2521 gaaaataatg atagcccgaa agcacaaagc ttttcagccg gccaccaaat cccatcagca
     2581 atttttaatg tcttattttg aaaaaacaag ggctctaaag gataaatatc catttctgca
     2641 attggatgaa gaactggttt atttttctca aacctgaatt actttataaa ttgtcctggt
     2701 tgagtaagtt tccccgggtc ttaaaatagt agttggaaaa tgaggaatat tgattgcgtt
     2761 aggatgttta tgtgtttcaa gacaaaggcc tgaaaacgca ccatagcttg tcccgttctt
     2821 cccttttact gtgggtatcc atttgccggt gtaaaaatgc actattggct ccgttgaata
     2881 tacctgcagc agcagtttgg aagcagcaga ctttacttca gccattaatg aaagtgatgt
     2941 ttcctccttt tttttattgg caatataact tttgtcatac tcagcaattt ttgttagcct
     3001 ttcaccaacc cgtacaaaat cactaaaatt aaataaagta ttttctactg gtgtcagact
     3061 tccgtttgca acaagctctt catcctggtc aagcgttagc gaaccataaa tctttacttc
     3121 atgatcttta atgtcaccct cgccattatt aagattgaaa taaccatgat gggtcaggtt
     3181 tacaatggtt gttttatcgc aggtggctgt gtattggtaa ctcagttcat cattatcatt
     3241 taattcaaac cggataataa cttcaagatt gctaggataa ccttcttctc catccggact
     3301 gacatacttt aattcaagaa aacaatccgg agaatctccc attgcttttt tttgccatac
     3361 ttttttatca aacccttcaa gaccaccgtg gagttggttg tttccgttat tccggcttac
     3421 tgaatagttt ttatcatcaa tcataaattc tgcattcttt atcctgttgg caacacgacc
     3481 aacagcacat ccaaaccagg gatattgagc caggtaatct gctcctgcat agtcttcaat
     3541 tttttcgaat cccagcacaa tatcattgac tgacccgtca gcatttttca atttaaaaga
     3601 agtgatagtg gctcctaagt tagtaataag aactttggtg cctttcgaat tggatagtgt
     3661 gaacaggctg acttcctgcc cggatgaatg agaaaaacaa aattgttgat tgatcggcat
     3721 gataactttg taattgtaat ttacgtaatc agaatttaaa atgatagttg tgaattaaaa
     3781 actcttcaag attttttaat aaaataacct ggttcgtagc cctgtcaaaa ttgtgttcgg
     3841 ggtaactgat cctgtaataa atatcgccat ttaagtaatc agttagaaaa cgcaatgcct
     3901 gcatataaat cattaataac ccggaaagat gaatatattt tttctccgaa aaagttagct
     3961 gcccgtttaa tacttctgta taaccggata caatagcatc ataaaaatcc ttgcgaatat
     4021 tgagctcctc ataccggtta ctattttcgt tttcgctaca agccatagaa cgaatcatat
     4081 ctcctaagtc ggaaaaaaaa taaccaggca tcaccgtatc aaaatctaca ggacatatca
     4141 atttctcact ggctgtatca aataaaacat tactgatctt ggcatcatga tgcatcaccc
     4201 ttagccggaa ctcatctgac tcaattatag tttcgtaaaa attgacatat cgctccctca
     4261 gatttaaatc gtcaataagc tgttgacttt tacgtattct ttcgttcaaa ccattactca
     4321 gtgattgagt aaattgtctg aatcgcaaag acagattatg gaagtcaggt atggtaatat
     4381 gcaattggtt actatcaaat ttgctgaaac atgctgtgaa accggcaaat atgttagcca
     4441 caccctttgc ctgagaaatg ttatcggcaa tgttcagcgt ttttgtcccg ctaataaatt
     4501 caaatactct ccagtaatta ttttgaccat ccctgaagaa agtcttatcg ttaataaaat
     4561 gtttaggttc tggaataata aaagggatat cctcttcccg gaggtatttc cagaggagca
     4621 tataattttg ctgaagtttc tctggcctgg taaaaacatg atgattgatt tgctggagta
     4681 aaaaagagct ccctgtatcg ttgccggtta ttttaaaaga ttgattgatg agtccgtttg
     4741 tgactggctc aacagtaaat tcatgggagt gatcagcaaa agctttcagt gcttctttat
     4801 aggatccttg attcatcggc acctgaaatt agccaatcaa tatgaccaat cccccttcaa
     4861 tcgtttgcgg aaaattttca tctgcttaat gtattctgtc acctcttacc tccaggttat
     4921 gaataatatc agcttcatag tccagccatt ctttccatct gcggcgaact ttgtctttac
     4981 tgatgtatgt ttcagccagt tcaataaata gtgtataatg cccggcttca ctttccataa
//
LOCUS       DAORTI010000196         4903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_21315_length_4903_cov_6.413779, whole genome shotgun sequence.
ACCESSION   DAORTI010000196 DAORTI010000000
VERSION     DAORTI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21315_length_4903_cov_6.413779"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1450)
                     /gene="der"
                     /locus_tag="PLO70_15730"
     CDS             complement(137..1450)
                     /gene="der"
                     /locus_tag="PLO70_15730"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQZ75971.1"
                     /translation="MSFTVAIVGRPNVGKSTLFNRLLEQRKAIVDDISGVTRDRQYGV
                     TDWNGKTFNVIDTGGFVLGSEDVFEKEIAKQVLVALEEANAIIFMVDAATGMTSLDDS
                     MANVLRKSTKPVFLTVNKVDTNERLLEASEFYSLGFDNIFFIAAASGSGTGELLDAIA
                     ELISEDASEETKEIAALPKFAIIGQPNTGKSSLLNALVGEERTIVSDIAGTTRDTIHT
                     HYNLFQKEFVLIDTAGIRRKAKVHEDLEFYSVIRAIKAVDEADVCLLLLDAEKGIAAQ
                     DLNIFSLAAKKGKGIVVLVNKWDLISKETNTAIEYEKELKKRFAPFSDVPVLFISAKE
                     KTRIFKAIEIALQVYDNRQQRISTSELNDIMLKAIQSYHPPVVRGNPIKIKFITQLPT
                     AVPSFAFFCNFPDDIKQPYKNYLENQLRENFNFTGVPVRIFFRKK"
     gene            complement(1462..2328)
                     /gene="era"
                     /locus_tag="PLO70_15735"
     CDS             complement(1462..2328)
                     /gene="era"
                     /locus_tag="PLO70_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQZ75972.1"
                     /translation="MKSGFVNIFGRPNAGKSTLLNALMGEKLAIVSPKVQTTRHRIKG
                     ILTEKDYQIIFSDTPGIIEPRYKLHEKMMLAVKSALEDADLAMLMVDVKEKWDECDQI
                     FSALQLKVPAIVVINKIDLASEEKIKEAVDFFTEKAYCKKVITISALSGISRKKLINA
                     ILEFLPEGEPFYEGDDISDLNTRFFVSELIREKIYELAEDEIPYHTAVLVREYQEKTT
                     LVKIVADIIVHRETQKAILIGDKGSMIKKIGTAARKDIEAFIQQKVFLELFVKVKPKW
                     RENDLYLKEYGY"
     gene            complement(2457..2528)
                     /locus_tag="PLO70_15740"
     tRNA            complement(2457..2528)
                     /locus_tag="PLO70_15740"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2494..2496),aa:Met,seq:cat)
     gene            complement(2631..3332)
                     /locus_tag="PLO70_15745"
     CDS             complement(2631..3332)
                     /locus_tag="PLO70_15745"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQZ75973.1"
                     /translation="MKKIAVLVAGFIFSLGLSAQINDPNAQVRQVKNFHGINVSNAFD
                     VYINQSNDEAVAVSAATVKDREMITVEVKEGILYVGLHKDWKWNTGKKKLKAYISFKQ
                     IDNLTVSGACDVFIAGILKADNLSINQSGASDIKGKLEVVKLRVDLSGASDMTVTGKA
                     TQLSVEASGASDFKGYDLVTETCDAKASGASDIKVTVNIELSAQASGASDVKYKGNGT
                     IRDIKSSGSSSVSRG"
     gene            complement(3355..4074)
                     /locus_tag="PLO70_15750"
     CDS             complement(3355..4074)
                     /locus_tag="PLO70_15750"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQZ75974.1"
                     /translation="MKKLLLSFLLVLGASSVLFSQKTINDPNAEKRNVSGFHGIEVAT
                     GIKLILTEGSTENVAVSAVTTEFRDKIVTKVENGILKIHYDSKLGAVNKRKESKGLKA
                     YVSYKTLDLLYATTGAEVEINEVLKSSTLDLKANTGALVNGKVDITTLKVSQNTGSKV
                     TLTGRAGKLDIDGDTGSKFKGEDMHTANCNVIVSTGAIVSVMAEKELQVKANTGGVVK
                     YKGNAAIREIKTNTGGSVSKI"
     regulatory      4191..4298
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4350..>4903
                     /locus_tag="PLO70_15755"
     CDS             4350..>4903
                     /locus_tag="PLO70_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQZ75975.1"
                     /translation="MKRIIMFGCFCFSAISAIAQQKEESKDSFYTLTPVEVKAIRAGE
                     NSPFTKTNLSKKEITKNNLGQDIPFLLNQTPSVVVNSDAGNGIGYTGIRIRGTDATRI
                     NVTLNGIPYNDAESQGTFFVDLPDFSSSTGSIQIQRGAGTSSNGAGAFGASINFSTNE
                     VNKEAYTEFNNSYGSFNTLKNTLK"
BASE COUNT         1415 a         1079 c          809 g         1600 t
ORIGIN      
        1 cgataattgc gaatactttt ttcattgtat tttttttatt aagtttaaaa attcaggtgc
       61 aaaactaaaa tcttttctga aaatttaaaa tattcatcag actttcttca aatagactgc
      121 caaaaacaga cttttactac ttctttctga aaaatatacg taccggaacc ccggtaaaat
      181 taaaattctc ccttaactga ttttcgagat aatttttata tggctgcttt atatcgtcag
      241 ggaaattaca gaaaaaagca aaggagggca ctgcagtcgg caattgggta ataaacttta
      301 ttttgatcgg atttcctctt actaccggcg gatgataaga ttgtattgct ttaagcataa
      361 tatcgttcag ttcagaagtg gaaattcttt gctgacggtt atcataaacc tgtaaagcta
      421 tttctatagc tttaaatatt cttgttttct cttttgcgga aataaataag acaggaacat
      481 cactgaaagg ggcaaaccgc ttctttaact ctttttcata ctcaatagca gtattagttt
      541 cttttgatat aaggtcccat ttatttacca gtacaacgat acctttacct ttttttgcag
      601 ctaaactgaa aatattcaga tcctgtgcgg caattccttt ctcagcatcc agcagcaata
      661 aacaaacatc tgcttcatca acagctttta tagcacggat gacagagtaa aattcaagat
      721 cttcatgaac cttagccttc cggcggatac cggctgtgtc aatcaaaaca aactcttttt
      781 gaaaaaggtt gtaatgagta tggatagtat ctcttgtagt acccgcaata tcacttacaa
      841 tggtcctctc ttcacctacc aatgcattca ataaagatga ttttcctgta ttaggctgac
      901 caataatagc aaattttggt aaagcagcta tctcctttgt ttcttcagat gcatcttcag
      961 aaatgagctc agctatggca tctaataatt ctccggtacc acttccacta gctgccgcaa
     1021 tgaaaaaaat attatcaaac ccaagactat aaaattctga ggcttccaat aaccgttcat
     1081 tcgtatcaac tttattaacg gtgagaaaga ctggctttgt actcttccgt aaaacattag
     1141 ccatggaatc atcaagactt gtcattccgg tagcagcgtc aaccataaaa atgatggcat
     1201 tggcttcttc aagagcgact aatacctgct tggcaatttc tttttcaaac acatcttcac
     1261 tccccagaac aaaaccacct gtatcaatta cattaaaagt tttcccattc caatctgtaa
     1321 caccatactg tctgtcgcgg gtaacaccgc tgatatcatc tacgatggct ttgcgttgtt
     1381 ccagtaaacg gttaaagagt gtacttttgc ccacattggg tcttcctact attgctactg
     1441 taaatgacat aaaaatatta attaataacc gtattctttt aagtatagat cattctctct
     1501 ccacttcggt ttcactttta cgaataattc taaaaaaact ttctgttgaa taaaagcttc
     1561 aatatccttt cttgctgcag tgccaatctt ttttatcata cttcctttat cacctattaa
     1621 tatcgccttc tgggtttccc tgtgaacgat aatatcagct acaattttca ccaatgttgt
     1681 cttttcctgg tactctctca ctaatacagc ggtatggtag ggtatctcat cttctgcaag
     1741 ttcatagatc ttttcccgga taagttcact tacaaaaaaa cgggtattta aatcacttat
     1801 atcatcgcct tcataaaatg gctctccctc tggcaaaaac tcaaggatcg cattgatcag
     1861 cttctttcgg cttatacccg acaaagcaga aattgtaata acttttttgc agtatgcttt
     1921 ttccgtaaaa aagtctactg cttccttaat tttttcttca ctcgccaggt ctattttatt
     1981 aataacaaca atagccggga cctttaattg cagcgctgag aatatctgat cgcactcatc
     2041 ccacttttct ttaacatcta ccatcagcat ggcgaggtct gcatcttcca gagcactttt
     2101 cacagccagc atcatttttt cgtgcagttt gtatcttggt tctataatgc cgggagtatc
     2161 tgagaaaatg atctggtagt ctttttccgt taatatccct ttaatgcgat gccttgtggt
     2221 ttgtacttta ggcgatacga tagccagttt ctcccccatc aatgcattaa gaagggtgct
     2281 tttaccggca ttcggccggc caaatatgtt tacaaatcct gatttcatga tatacctgta
     2341 ggtgcacact tcaggtagca agaagatgag gaggtagtca atccattgcc agttgaaggt
     2401 gcaactgttt cggaaaattt aaaaaagcca tctgcattgc ggatggcttt ctgttgttgc
     2461 gaaggggagg atcgaactcc cgacctttgg gttatgagcc caacgagcta ccgctgctct
     2521 acttcgcgat gttggacggc aaaggtaacc gtattgaaca aactttccaa agaaaaaacg
     2581 ccccaataaa atcaggacgt tttctcatgg ctgtacttat aaataaacaa ttatcccctg
     2641 gaaacactgc ttgaaccact gcttttaata tcacggatgg ttccattccc tttgtacttt
     2701 acatcactgg caccactagc ctgtgcggac agttctatat tgacggtaac ttttatatcg
     2761 ctggcaccgg aagcctttgc atcacaggtt tcagtcacaa ggtcgtaccc cttaaaatca
     2821 ctggcaccac ttgcttccac tgaaagctgc gtggccttac ctgttactgt catatccgaa
     2881 gcaccgctta aatccaccct tagtttaaca acctccagtt tacctttgat atcacttgct
     2941 ccagattgat ttatagataa attatcagct tttaatatgc ctgcaataaa cacatcacaa
     3001 gcaccactga ccgtcaagtt atctatttgc ttaaaagaaa tataggcttt cagtttcttt
     3061 tttcccgtat tccatttcca gtccttatga agtcccacat acaggatacc ttctttgacc
     3121 tctacagtga tcatttcccg atcttttaca gttgctgcac ttactgcaac agcctcatca
     3181 ttactttgat taatatacac atcaaatgca ttggagacat tgataccgtg aaaatttttc
     3241 acttgtctta cctgtgcatt cgggtcattt atctgggcag aaagacccaa gctgaaaata
     3301 aatcccgcaa ccagtactgc tatctttttc ataaatttta tatcttataa gtaattaaat
     3361 ttttgataca gagccgccgg tattagtttt tatttccctt attgcagcat tgcctttata
     3421 cttcacaact cccccggtat ttgcttttac ctgcaactct ttttctgcca ttactgaaac
     3481 aatagcacca gtgctgacta taacattgca attggccgta tgcatatcct ctcctttaaa
     3541 tttactgcct gtatctccat caatatcaag tttgcctgcc ctgcctgtaa gcgtaacttt
     3601 tgatcccgta ttctgactta ctttaagtgt agtaatatcc actttaccat ttaccaatgc
     3661 gcctgtattt gctttaaggt ccaaagtcga tgatttaagc acttcattga tctctacttc
     3721 agctcctgta gtagcatata acaaatcaag tgttttgtat gacacataag ccttcagacc
     3781 cttgctctct ttccgtttat taacagctcc taatttggaa tcataatgga ttttgagtat
     3841 cccattctct acttttgtaa ctattttatc tctgaactca gtagtaacag cacttactgc
     3901 cacgttttca gtactaccct ctgtcaaaat caatttaatt ccggtagcta cttcgatgcc
     3961 gtgaaagcca cttacatttc tcttttctgc atttggatcg ttaattgttt tttgggaaaa
     4021 aaggactgaa ctggcgccta agaccaacaa aaaggacaat aataacttct tcataaacta
     4081 tggtttagat gtgatacgaa ggacagcgta gaaatgttac atatctaata aaaatattct
     4141 tcttactttt gttctagttc ttggcctttt aatcaaaagg ctttgatttt cagtgtttcc
     4201 cggggtgttt atttcgccaa ctggcggaat aatctgagat catacccgtt gaacctggtc
     4261 agggtaatgc ctgcgaaggg aaggagttat gagttgtcct ttctccaaca gcgtttccct
     4321 cttatttttt aactattaaa tgtaaaaaaa tgaagaggat tattatgttc ggctgtttct
     4381 gtttttcagc cattagtgcc attgcacaac aaaaagaaga atcaaaagac agtttttaca
     4441 ccttaacacc tgtagaagta aaagctattc gtgcaggcga aaattcacct ttcaccaaaa
     4501 ccaatctttc aaaaaaggaa attacaaaaa acaatctcgg gcaggatatc cccttcctgc
     4561 taaaccagac accgtctgta gttgtaaatt ctgatgctgg taatggaatt gggtatacag
     4621 gtatccgcat ccggggtaca gatgcaacaa gaatcaatgt taccctgaac ggcattcctt
     4681 ataacgatgc ggagagccag ggaactttct ttgtcgatct gcctgatttt tcctcctcaa
     4741 ccggcagtat tcagatacaa agaggggcgg gtacttcctc caatggagct ggtgcattcg
     4801 gggccagtat taatttcagt acaaacgaag tcaataaaga agcttacaca gaatttaata
     4861 acagctatgg ctcatttaac acattgaaaa acacgttgaa att
//
LOCUS       DAORTI010000197         4867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_21552_length_4867_cov_6.490441, whole genome shotgun sequence.
ACCESSION   DAORTI010000197 DAORTI010000000
VERSION     DAORTI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21552_length_4867_cov_6.490441"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLO70_15760"
     CDS             <1..344
                     /locus_tag="PLO70_15760"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQZ75976.1"
                     /translation="TEASSSEFFFIKADNKLVKLLIDEVLFVEAMQNYVMINTSGKKY
                     ITYLTFKALEDYLPADKFLKVHKSYIVALSKVDSIEGNEIRIGQHHIPISRNQKEEVM
                     DKLLKNRFLKR"
     gene            386..1384
                     /locus_tag="PLO70_15765"
     CDS             386..1384
                     /locus_tag="PLO70_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQZ75977.1"
                     /translation="MKYLLYILLLFASPGFSQSSQISKEEENPEALAKKITAGCTSDR
                     ERVNRIFRWITGNISYRVKTGQNKAVTGTASLKYSNGEVDDDKPLKPLNERVAEMVLK
                     RKEAVCDGYARLFSTLCQYSGIRSEIIVGYARNNINKPLPKFRVNHTWNAVFLEDEWH
                     LLDVTWASGYISRNGNEFVRNYDSRYFLTPPEDFIRDHYPDDPRWTLLPDAEVPEEFR
                     NSPFKQKSFVKYSITSFSPAVGVIEATVGDTIYLELATADADRDKQISPDMSVDSTIF
                     QHSDSWVFLKPVVADKAPLLPRIYKYVYSIASADVKWLYLLYNDDMVLRYKLYVKQ"
     gene            complement(1416..1832)
                     /locus_tag="PLO70_15770"
     CDS             complement(1416..1832)
                     /locus_tag="PLO70_15770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HQZ75978.1"
                     /translation="MTKEELLKELSEEIYAVLKPSPVHGIGVFALRDIPKGCRNIFSK
                     NIGEWIKLPIAAIEQLPEHSRNLVETYCLYDDENYYVPDYGFKVMDLVNYLNHSATPN
                     IISINNGECFEALTNISAGTELLINYGEIVETGEYD"
     gene            complement(1850..1999)
                     /locus_tag="PLO70_15775"
     CDS             complement(1850..1999)
                     /locus_tag="PLO70_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75979.1"
                     /translation="MSLLNQKNKKGKKKEDKSKSAANQGSKFIQKPKSGFVSKQQNTG
                     SQRGS"
     gene            complement(2029..2520)
                     /locus_tag="PLO70_15780"
     CDS             complement(2029..2520)
                     /locus_tag="PLO70_15780"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQZ75980.1"
                     /translation="MRPVIVFLFFTILFLSACNNNKTEKAASLNEPSASPGTDSSPVV
                     TTSPLNGSWDLINITDLKIALSKLYPRQKPNLNFDLASGVLTGSTACNRFSGPVKIID
                     SSISIGELGMTKIACPGDGEQKFLEALKKTTTYALVDSVTLSLRKGDAEVMRFLRKQK
                     VIL"
     gene            complement(2593..3120)
                     /locus_tag="PLO70_15785"
     CDS             complement(2593..3120)
                     /locus_tag="PLO70_15785"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HQZ75981.1"
                     /translation="MTRTFYLLLILVLPCVSNAQSWKIFADTGIAFTAKYPSSWVNKI
                     KEEKRVFFTSPSDGEKDDFRENINISVKTNAAYGTELKIKEVVSTVIESLKKSIDNFN
                     LQAENYFKWNNADAVELIYGGTYDINGTSTEVRITQWFCFWNTKLYTATYTALKGVNT
                     HDETAKKILKSILFK"
     gene            3253..4080
                     /locus_tag="PLO70_15790"
     CDS             3253..4080
                     /locus_tag="PLO70_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75982.1"
                     /translation="MRQLFFAAILVMTSLLSAAQPASALKKLKKGISLKVARSESDEM
                     PGTRGASVAWHPGLKRYYAAMAGNVGYPLSVFDATGKRVSPDSLNCEADVRGLWYNPV
                     AKTIQGNAYGDGGWFSYKLDAKGMVKEVESLMEEMTQPNEQSVGAYNPVAAQVLFLDG
                     SKVFFYNMNDGLSEDFVNIHWGRTKAEGASEEEDETVTPVSYNTSLVFTGSKGAELGF
                     LNIDENQIELYNIEDGFLTRKLQLPEGTASYQSFNFAFSNGMYWLFNIETRTWTSYK"
     gene            4203..4706
                     /locus_tag="PLO70_15795"
     CDS             4203..4706
                     /locus_tag="PLO70_15795"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ75983.1"
                     /translation="MITAVELVNRFNMLPHPEGGYYARTYSSSGFVPVNVLPGGFSGS
                     RPFSTAIYFLLEKGNFSAFHRIKSDECWHFYTGQCLLIYVIDPRGVLTTIKLGTTINE
                     GETFQFVVPAGCWFASEPAPSSSFSFVGCTVAPGFDFEDFELAYTNELVKLYPQHQSL
                     IKRLCRQ"
BASE COUNT         1404 a          898 c         1048 g         1517 t
ORIGIN      
        1 taacagaagc atcctcttct gaattctttt ttataaaggc ggataacaag ttggttaagt
       61 tattaattga tgaagtattg ttcgtggaag ctatgcagaa ctatgttatg atcaatacct
      121 ccgggaaaaa gtacatcacc taccttactt ttaaagcgtt ggaagattac ctgccggctg
      181 ataaattttt aaaagttcac aaatcatata ttgttgcgct gtcaaaagtg gacagtattg
      241 aaggaaatga aatcaggatc gggcaacacc atattcctat cagccgaaat cagaaagagg
      301 aagtaatgga taaactgctt aaaaaccgat ttttgaaacg ctgagggttt gaaaaccggc
      361 ctaatcattt taattttagt gagtagtgaa atacctgttg tacatattat tattgtttgc
      421 cagccctggt ttctctcaat ccagccagat cagcaaagaa gaagaaaacc cggaggcatt
      481 agcaaaaaag atcactgcag gttgtacttc cgaccgggaa agggtaaaca ggatattccg
      541 atggatcacc ggtaatattt cttacagggt gaaaacaggg caaaataaag ctgttaccgg
      601 aacagcatca ctaaagtatt cgaatggcga agtggatgac gataaaccac tgaaaccatt
      661 gaatgaaagg gtagctgaaa tggtactgaa acgaaaggaa gccgtttgtg atggctatgc
      721 ccggcttttt tcaaccttgt gtcaatacag cggaatacgg tcagagatta tcgtcgggta
      781 tgcaagaaat aatataaata agccgcttcc aaagtttcgt gtaaaccata cctggaatgc
      841 tgttttcctg gaagatgaat ggcatctcct ggatgtgacc tgggccagtg gctatatatc
      901 aagaaatgga aatgagtttg tcaggaatta tgattcccgg tattttctta ctcccccgga
      961 agactttatc cgcgaccatt atccggatga tccgaggtgg accttacttc ctgatgcgga
     1021 agtgccggag gaattcagga attcgccatt taagcaaaaa tcatttgtaa agtacagcat
     1081 cacctcattc agtccggctg ttggtgtaat cgaagccaca gtgggtgata ccatttatct
     1141 tgaactggca acagccgatg ctgaccggga caagcagatc tcaccggata tgtctgttga
     1201 ttcaacgatc tttcaacact cagattcatg ggtgttttta aaaccagtgg tggcagataa
     1261 agcgccgtta ttaccccgga tttacaaata cgtatactct attgcttctg ctgatgtaaa
     1321 gtggttatac ttgctttaca atgacgatat ggtattacgt tataagctgt atgtaaaaca
     1381 atgaaaagag aggttgctgt tagcaattga aatgatcagt catattcccc ggtctccact
     1441 atttcaccgt agttgatcag caattctgta ccagcagaaa tatttgtcaa cgcctcaaag
     1501 cattctccgt tgtttattga aattatgttg ggtgttgctg aatgattgag ataatttacg
     1561 agatccatta ctttaaaacc gtagtctggg acgtaataat tttcatcatc atagaggcaa
     1621 tacgtttcta ccaggttacg ggaatgttct ggtagctgtt caatggctgc aatcggtagc
     1681 tttatccatt caccgatatt ttttgaaaaa atattccggc agccctttgg aatatccctt
     1741 agcgcaaata caccaatccc gtgaacggga gagggtttca gcacagcata aatttcctcc
     1801 gaaagttctt tgagtaactc ttcctttgtc attgtgcaat aaaattggat caactgcccc
     1861 gctgtgaacc tgtattttgt tgcttactca caaagccaga tttgggtttc tggataaatt
     1921 tagagccctg gttggcggcc gatttacttt tgtcttcttt cttttttcct ttcttgttct
     1981 tttgattcag taatgacata ggagtatttt ttttataaag ataattgttt ataaaatcac
     2041 tttctgcttt cttaaaaatc gcatcacttc tgcatcacct ttacggaggg agagggtcac
     2101 actgtctacc agtgcatacg tagttgtttt ctttaatgct tccaggaatt tttgctcacc
     2161 atcaccggga caggcaattt ttgtcattcc cagttctccg atactgatgc tgctgtcaat
     2221 aatttttaca ggacctgaaa acctgttaca agccgtgctg cctgtcaaca ccccgctggc
     2281 aagatcaaaa ttaagatttg gtttctggcg gggatatagc tttgacaggg caatttttaa
     2341 atcagttata ttgattagat cccatgatcc attcagggga gaggttgtta caacgggtga
     2401 tgaatcagtc ccggggctcg ctgatggctc atttagtgat gccgcttttt cggttttgtt
     2461 gttattgcag gcggatagga ataaaatcgt aaagaataaa aagacaatta cgggcctcat
     2521 ctgattgtgt tttgatggca aaattaaaaa gtaaatacaa attataaaat gctaagtaaa
     2581 gtttaaatcg gatcatttga aaagaatgct tttcaatatc tttttagcag tttcatcatg
     2641 tgtattaaca cctttcagag ccgtgtaggt tgctgtataa agttttgtat tccaaaagca
     2701 aaaccattga gtgatgcgta cttcggtgga agtgccattt atgtcataag tgccgccata
     2761 aatgagttca acagcatcag cattattcca tttaaaataa ttttctgcct ggagattgaa
     2821 gttatcgatg cttttcttta atgactcaat gacggtggat actacttctt taatttttaa
     2881 ctcagtgcca taggcagcat tggtttttac gctgatattg atattttcac gaaaatcatc
     2941 cttttctccg tcagaggggg acgtaaaaaa aactcttttt tcttccttga tcttatttac
     3001 ccaactggaa ggatatttgg ctgtaaaggc aatacctgta tctgcgaata ttttccagga
     3061 ttgcgcatta gatacacacg gcaacacaag gataagtagc aaataaaagg ttcgggtcat
     3121 tagattaata ttatttaaac ttcaaagacg aatgtagttg tatttgatca aagttactat
     3181 tgaataaaat acccggtgca ggggatatct ttaacgcttc attttgactt actttacacc
     3241 aaaatcttag atatgagaca actctttttt gctgccattc tggtgatgac atctttactt
     3301 tctgctgcgc agccagccag tgcccttaaa aaactcaaaa agggaataag cctgaaagtg
     3361 gcaaggtcgg aaagtgatga aatgcccggc accaggggag catcagtagc ctggcatccg
     3421 gggttgaaaa gatattatgc tgccatggca ggtaatgtgg ggtatccatt atctgttttt
     3481 gatgcaacag ggaaaagggt ttctcccgat agcctgaatt gcgaggccga tgtaagggga
     3541 ttatggtata accctgtggc taaaacaatc cagggcaatg cgtatggtga tggcgggtgg
     3601 ttcagctata aactcgatgc aaaaggtatg gtgaaggagg tcgaaagcct gatggaagaa
     3661 atgacacaac cgaatgagca gtctgtcggg gcctataatc ctgtagcagc acaggtgctt
     3721 ttcctggatg ggagcaaagt gtttttttat aatatgaatg atgggttgtc tgaagatttt
     3781 gtaaatatcc attggggccg tacaaaagca gaaggagctt ctgaggaaga ggatgaaaca
     3841 gtgacaccgg ttagttataa tacatcgctt gtttttacgg gtagcaaagg ggctgaactg
     3901 ggctttttaa atattgatga aaaccagatt gagctctaca atattgagga tggattcctt
     3961 accaggaaat tacaattgcc ggaagggact gccagttacc agtctttcaa ttttgccttt
     4021 agtaatggga tgtactggtt atttaatatt gagaccagga catggaccag ctataaataa
     4081 ttaatttatc atggttttta ataaagtccc tgttcaatac ggggacttgt tatatacggc
     4141 aattattaca cccgaattta attagtttag tcctttgttt ttcaactcca caatatttct
     4201 ctatgatcac ggccgttgaa ttagtcaata ggtttaatat gttgcctcat ccggagggag
     4261 gttattatgc acgaacgtat tcctcttccg gttttgtgcc ggtaaatgtt ctgcccggag
     4321 gtttttcagg aagccggcca ttttcaacgg ctatttattt cctgctggag aaagggaatt
     4381 tttcagcatt tcaccggata aaaagtgatg aatgctggca tttttatacc ggtcagtgtt
     4441 tgctaatata tgttatcgat ccccggggag tattgacaac tattaagttg gggacaacta
     4501 ttaatgaagg cgaaactttt caatttgttg tgcctgcggg ttgctggttt gccagtgaac
     4561 cagctccatc atcatcattt tcatttgtag gatgtacagt agcacccggt tttgattttg
     4621 aagattttga actggcttac acaaatgaac ttgttaaatt atatccgcag caccaatcac
     4681 taattaaaag actctgccgt caatgacatt tttattcaat tgtgctatcc gggtttctta
     4741 aagctcaaca aaggaacttt taaagtatga gccatatctt ccgttttact gccgataatt
     4801 agcttatccc ataagctttt ttctttagta aacattgtca gccattcggg ttgatgttcc
     4861 tgcatat
//
LOCUS       DAORTI010000198         4727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_22549_length_4727_cov_5.907962, whole genome shotgun sequence.
ACCESSION   DAORTI010000198 DAORTI010000000
VERSION     DAORTI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22549_length_4727_cov_5.907962"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4015
                     /locus_tag="PLO70_15800"
     CDS             <1..4015
                     /locus_tag="PLO70_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ75984.1"
                     /translation="ELPRHIHGNIPLLAKWEKGKKPTVVYAMNVEEALDYVHKMVSLG
                     DFKNIDPQAPSEDDIRHIGGLTFIMARTTHGEIGFSLPGVVEHPPKEDVDILIRFAGA
                     FDIAHRRFLDLQQSEAQSREVEIELALEKVRSRSMGMQKSEELNEAIKIVYQQLNHLK
                     IKLDHAGFVVDYTPKADWHFWIADEQDIPSKITHPFFESVWASQFNEAKDKGADLFAT
                     HLNFEEKNKFYKELLSYVPDLPEASKDFYLSCPGLAASTVLFDNVSLYIENFEGIPYT
                     DDENKILLRFGKVFQQTYTRFLDLQKAEAQAREAQIETALEKVRSRSLAMHKSEELGE
                     VITVVVEKLKDLGFFVGDGIALITFSKGSKDLVEWMTNPGFASAIHFNLPHFDHPVLN
                     NLWEAKERGEVFNFTRFSAEENKSFLDHIFEHSDFKHTPQEVKEYCLAANTYATSISF
                     QENTAIFINDYSGKSLSEHEVNILNRFAKVFEQSYTRFLDLQKAEAQAREAQIETALE
                     KVRSRTMAMQKSDELSETAAVLFQEFKKLGNDSIYQVTIGIYNEAEKLIEFRVTSWAG
                     GGEQISKPFMLSMDEPTLLKPAVIAWKANEKLMVADLTGEALEGWLNYRNKMTGTIVS
                     SADTEGRRVISIAFFSKGHLSLSSPVPLPDGTLKTLERFAAVFDGTYTRFLDLKNAEA
                     QAREARIEMALEKIRSRTMAMQHSNELPEAANLLFLEVQALGIPAWSCGYNILAEDRT
                     TATCWMSSEGTLQIPFKLRLFGEASFAEMGDFIRSEQSMLVQELSNQALGDHYAYMKS
                     FPDLKATFDQIDELGLSLPAYQINHLCKFTQGFLLFITYEQVPHAHDIFKRFTKVFEQ
                     TYTRFLDLKKVEAQARQSQIEAALERVRARALAMQQPEELKDVAKVLRHEMGLLGIEE
                     LETCSIYINDEQSEKAECWYALKDMRSEEKKLVNDHFALNLNDTWVGREMLSFYNSAI
                     KQISVVMQGANRVEWIRYCEASSTPFRGYYGDVIPDRTYHLYKFSHGAIGAASAGDIS
                     IESWSLLKRAASVFSLAYSRFKDLTQARVDLQKLKEEKQRAEDALSNLQAAQKQLVQS
                     EKMASLGELTAGIAHEIQNPLNFVNNFSEVSKELLDEMREELKKGNQEDAMEIMNDVI
                     QNLEKINHHGKRADGIVKGMLQHSRSSSSTKESTDINALCDEYLRLSYHGLRAKDKSF
                     NATMKTNFDNSIGNINIIPQDIGRVVLNLLTNAFYVVDEKKRSGIENFEPTVSISTKN
                     VNDKVEIKVTDNGNGIPQKVLDKIFQPFFTTKPTGKGTGLGLSMSYDIVTKGHGGDLS
                     VETVENEGSTFTISLPI"
     gene            4147..4581
                     /locus_tag="PLO70_15805"
     CDS             4147..4581
                     /locus_tag="PLO70_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75985.1"
                     /translation="MKKFLLPILFLLSITVHSQNSELTVIGNKNGVPAQLSTENLKSI
                     FMGNQPKWSDGSKVIIAMMKFTTDGGKTTCSKLYQKSTTDVTKHWLTVSMKGVIDAPV
                     FFNTAAELQNFVANTAGAIGVFDGTVSASTVKTVLIDGKRSF"
BASE COUNT         1563 a          845 c         1018 g         1301 t
ORIGIN      
        1 ggagcttcca aggcatatac atggcaatat tccattgctg gcaaaatggg aaaaaggcaa
       61 aaagccaact gttgtatatg caatgaatgt agaagaagca ctggattatg tacacaaaat
      121 ggtttcgctt ggcgatttta aaaatataga tccgcaggct ccaagtgaag atgatatccg
      181 gcatatcggc ggacttactt ttatcatggc gagaacaaca cacggagaga ttggtttcag
      241 tttaccggga gtggtagaac atccgccaaa ggaagacgtc gatattctta ttcgttttgc
      301 cggtgcattt gatatagcac atcgtcgttt tctcgatctg caacaatcag aagcacagtc
      361 cagagaagtt gaaatagaac tggccttgga aaaagtgaga agccgttcta tgggtatgca
      421 aaaaagtgaa gagttgaacg aagcaataaa aattgtttat cagcagctca atcatttaaa
      481 aattaagctt gatcatgcag gatttgtggt tgactatacc cccaaagctg attggcattt
      541 ctggatcgct gatgaacagg acattccttc aaaaattacg catccctttt ttgagtctgt
      601 ttgggccagt caatttaatg aagcaaaaga taaaggtgca gatctctttg ctacccattt
      661 aaattttgaa gaaaagaaca aattttacaa ggagctttta tcctatgttc ctgatttacc
      721 ggaagcatca aaagattttt atttaagctg tcccggcctt gcagcatcaa cggtgttgtt
      781 tgataatgtt tccctctaca ttgaaaactt tgagggcatt ccttatacgg atgatgagaa
      841 taaaatattg ttacgttttg gaaaagtatt ccaacaaacc tatacccgtt ttctcgatct
      901 gcaaaaagcc gaagcacagg caagagaagc acagatagaa acggcacttg aaaaggttcg
      961 atcacggtca ttggccatgc ataaaagtga agaattgggg gaagtgatta cggtagtggt
     1021 ggagaaattg aaggacctgg gtttttttgt gggagatggg atagccttga ttaccttttc
     1081 caagggatca aaagatttgg tagaatggat gacaaacccc gggtttgcaa gtgctattca
     1141 ctttaacctt ccccattttg atcatcctgt tttgaataat ctttgggagg caaaagaaag
     1201 aggagaagta tttaatttca caaggttcag tgccgaagag aataaatcat tcctggacca
     1261 catttttgag cattccgatt ttaaacatac tccgcaggaa gtaaaggaat attgtttggc
     1321 tgccaacacc tatgctactt ccatatcctt tcaggaaaac acagccattt tcattaatga
     1381 ttattcgggt aaatcacttt cggaacatga agtaaatata ttaaatcgtt ttgcaaaagt
     1441 tttcgaacaa tcctacaccc gcttccttga tctgcaaaaa gccgaagcac aggcaaggga
     1501 agcacagatt gaaactgcac tggaaaaagt tcgtagcaga acaatggcca tgcaaaaatc
     1561 agatgagctg tcagaaaccg cagctgtact ttttcaggaa tttaaaaaat taggtaacga
     1621 ctctatttac caggttacaa taggtatata taacgaagcg gaaaaactga tagaattcag
     1681 agtaacaagc tgggcaggcg gcggtgagca aataagcaaa ccatttatgc tgagtatgga
     1741 tgaacctaca ttattaaagc cagcggttat tgcatggaaa gcaaacgaaa aattgatggt
     1801 agccgacctt accggggagg cattggaagg ttggcttaat tatcgaaata aaatgactgg
     1861 taccatagta agcagtgcag atacggaagg ccgcagggta attagcattg cctttttttc
     1921 caaaggacat ctgtcgcttt cgtcgccggt accactgcct gacggcacat taaaaacact
     1981 ggaacgtttt gctgctgtgt ttgatggtac ttacacccgt tttctggatt taaaaaacgc
     2041 cgaagcacag gcaagagaag caagaataga aatggcgctg gaaaaaatcc gttcccgcac
     2101 catggccatg cagcatagta atgagttacc cgaagctgcc aatttattat tcctcgaagt
     2161 gcaggcattg ggaattcccg catggagttg cggttacaat attcttgcag aagatagaac
     2221 aacagcaacc tgctggatga gcagtgaagg aaccttgcaa atcccattta aacttcgctt
     2281 atttggcgaa gcttcctttg ctgaaatggg tgattttatt cgtagtgaac aatcaatgtt
     2341 ggtgcaggag ctcagtaatc aggcgttagg agatcactat gcttatatga aatcctttcc
     2401 tgatctaaaa gccaccttcg atcaaatcga tgagcttggg ctttcgctgc ctgcttacca
     2461 gatcaaccac ctgtgcaaat tcacccaggg gtttcttcta ttcatcacct acgaacaggt
     2521 gcctcatgca catgatatct tcaaacggtt cacgaaagtt tttgaacaga catacacccg
     2581 tttcctcgat ctgaaaaaag tggaagccca ggccagacag tcacaaattg aagcggcgct
     2641 cgaaagagta agagcaaggg cattagccat gcagcagcca gaagaattaa aggatgttgc
     2701 aaaagtatta cggcatgaaa tgggcttgtt gggtatagaa gaactggaaa cctgcagcat
     2761 ttatatcaat gatgagcagt cagaaaaagc tgaatgctgg tacgcattaa aagatatgcg
     2821 atcagaagaa aagaaattag tgaatgatca ttttgcattg aatctgaatg atacctgggt
     2881 aggaagagaa atgctttcgt tttacaattc agcaataaaa caaatttctg ttgtaatgca
     2941 gggagctaac cgggtggaat ggatccgtta ttgcgaagcc agctccactc ccttccgtgg
     3001 ttattatggt gatgtgattc ccgaccgcac ctatcactta tacaaatttt cgcatggtgc
     3061 tattggggct gcctctgccg gagatatatc aattgaaagc tggagtctat taaaaagagc
     3121 agcctctgtt ttttcactgg cttattcccg tttcaaagat ttaacacagg caagagttga
     3181 tctgcaaaaa ttaaaagaag aaaagcaaag ggcggaagat gcactcagca atttacaagc
     3241 tgcacaaaag caattagtgc aatcagaaaa aatggcttcg cttggtgagc tcaccgcagg
     3301 tattgctcat gaaattcaaa acccattgaa cttcgtcaat aactttagtg aagtaagtaa
     3361 ggagctactt gatgaaatga gagaagaact caaaaaaggt aatcaggagg atgcaatgga
     3421 aattatgaat gatgtaattc agaatctcga aaaaataaat catcatggca aaagagctga
     3481 tggtattgta aaaggaatgt tgcaacattc aagaagcagc agtagtacaa aagaatccac
     3541 cgacatcaat gctttgtgtg atgaatattt gcgattgagt tatcatgggc tgcgggcaaa
     3601 agataaatca ttcaatgcaa cgatgaaaac taattttgat aattcaatag gcaatatcaa
     3661 catcattcca caggatatag gaagagttgt tttgaattta ttgactaatg cattttatgt
     3721 tgttgatgag aaaaagaggt caggaattga aaactttgag ccaactgttt caatcagtac
     3781 aaaaaatgta aatgataaag tggaaataaa agttactgac aacggcaatg gcattcctca
     3841 aaaagtattg gataaaatat ttcaaccctt ctttaccacg aaacctactg gaaaaggaac
     3901 gggattggga ttgagtatga gctacgatat tgttacaaaa gggcatggag gagatttatc
     3961 tgttgaaact gttgagaacg aaggttcaac ttttactatc agtttaccaa tatgaggcaa
     4021 taaaagaact gtttttcaaa ataagcagca gtaagataca accctgctcc ttgaattgca
     4081 ctcataaacg tatatctttg agagagagaa atgaatgttg gagattatta aacgaaagca
     4141 aaaaaaatga agaagtttct tttaccgatt ttatttcttt taagcatcac tgttcattca
     4201 caaaacagtg aacttactgt tattggaaac aaaaatggtg tacctgctca gcttagtact
     4261 gaaaacctga aatctatttt tatgggcaac cagccaaaat ggagtgatgg cagtaaagtg
     4321 attatagcta tgatgaagtt tactaccgat ggcggaaaga ctacttgcag caagctttat
     4381 caaaaaagta caacagatgt aaccaaacat tggcttacag tttctatgaa aggtgtaata
     4441 gatgctcctg tttttttcaa tacagccgct gagttgcaaa atttcgtagc gaatactgct
     4501 ggtgcaatag gagtttttga tggaactgtt tctgcctcaa ctgtaaagac agttttgata
     4561 gatgggaaga ggagctttta atttttactt tacatatata caagccaact aatcaattgc
     4621 aacaacttta ttccatacta tgttcatagg ggtaaaaacc aaaatattgc ttacagtatt
     4681 agcgattgtt ttactgttta ccattttctc tcttgtttac tttcctt
//
LOCUS       DAORTI010000199         4715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_22654_length_4715_cov_6.550215, whole genome shotgun sequence.
ACCESSION   DAORTI010000199 DAORTI010000000
VERSION     DAORTI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22654_length_4715_cov_6.550215"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLO70_15810"
     CDS             <1..574
                     /locus_tag="PLO70_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQZ75986.1"
                     /translation="TKYVYESGKAPNGDGIFVVDSSISNIGKSSPRHYYGADAQVKLK
                     QGWGETEWRAEYWFGTQPGTSASTSNPGTLPTSNGNPVPTYVRHFDGVFLLFLQNIVN
                     NRHQLMLKYDWYDPNIKVKDAQIGKSGTNLSAADIKFSTLGIGYAYLINPQTKLILYY
                     DIVKNEKTQLTGYTTDLKDNILTCRLQFRF"
     gene            678..3521
                     /locus_tag="PLO70_15815"
     CDS             678..3521
                     /locus_tag="PLO70_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ75987.1"
                     /translation="MYSRLRLLLSVLFITAFISLNAQSVKLSGKVMNEKNEPLAGANI
                     IITNGASKQAISDVEGKFYFTFEAGVKHTLVVSRVGYATKEISEVEALLNQDNYLEII
                     LKESSTTKLEGVTITATSRRQENTSALLSFQRNSTALSSGLAADFIRRTPDKNTGEVL
                     KRVSGTSIQDGKFVIVRGLSDRYNSAVINNAQLPSTEPDKKAFSFDVIPSALIDNIII
                     NKTATPELPGEFAGGLIQINTKDVPTRNLLSVGVSWGFNTQSVFKDFTSNERNGNDWL
                     GFDDRTRGLPKGFPTSAQAYRVLGGTTTGIQQQIEYSKLFNNDVYNEKNSTALPTQTY
                     NLTWGSGKKFKNGGTLGTILSLQYRNSMLKYDVERRLHENDGDVLVQLFDEQNRYSVN
                     VGALANITYIKGRHKISFKNLFNQLFEDNYYTRGGVSNDRIQDINFRSSVLNQRSLYS
                     GQVEGNHQLTNSGIKLMWNGNFAYNWKSQPDLRTSAYFRGKGTNDPFEFNDDDTRRFY
                     SDLKDYSYGANGSLSIPFTFAKQKQTFKAGGSTLIRIRDFRSRIFRYEPASISQFDAT
                     KNLLPYNQIFAPENMTTDGFKILDFTNNQDKYFGVSILNGMFGMFDNKFGEKVRLVWG
                     ARVENFQQFLTTRDVTAKRVVVNTEKWDVLPSFNFTLSPNSKHSIRIAGSRTVARPEF
                     REIAPFSFFDYEVNYAVNGNPDLKRSSILNGDIRYEFYPKGGEAITVGAFYKNFDDPI
                     ELRLNPSSVLDRRNYEYTNADKAITIGAEFEVRKNLDFLNQSLTNFSVFANLTYIYSK
                     VTLASTSSSGAAVSSNRPLQGQSPYLVNLGLQYNSKQGGWNGSLLYNRIGQRLALVGI
                     NDLGFPDVYERPRDQVDIQIAKKIFNNRGELKLTWADLLNPAYYFYENTDSKKAFSSG
                     NDRLFYSYKPGSTISIGFTYDFNVGKNNSFAKK"
     gene            3544..>4715
                     /locus_tag="PLO70_15820"
     CDS             3544..>4715
                     /locus_tag="PLO70_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75988.1"
                     /translation="MKKILFGALAFMALFSTPSCVKVNFDEGDAITPPVDPTSNIISG
                     VISSSKFYAKGKWILKGYVYVTEGATITFEAGCVVQSDITEKGALIIERGAKLIASGT
                     KDNPIVFTSGKAAGSRAPGDWGGIILLGKAPTNRPLDPAPTIEGGVGRQYGGTDPLDE
                     SGILRFVRIEFAGIAAEPGSEINGLTMGGVGSGTIIENVQVSFGNDDAYEFFGGTVNA
                     KNLIAFATADDDFDFDYGYTGKIQFAVSMRKPDFVDAGDAGNGIECDNDGSGTTATPI
                     TKPQLSNFTFVGPNDAPGTAGNHNFSNRWRRRAQFILRNSILIGHPDAGFSMESDGTL
                     NDYYVNNISEFKNNLVHATVLPYKTANAAIATNAQIQAKAESEGCVTLATSAAAQLT"
BASE COUNT         1406 a          925 c         1058 g         1326 t
ORIGIN      
        1 gaccaaatat gtgtatgaaa gtggaaaggc tcctaatgga gatggaattt ttgttgtcga
       61 ttcatccatc tctaatattg gaaaatcgtc tcccaggcat tattatgggg cagatgccca
      121 ggtaaaactc aaacagggct ggggtgaaac agaatggagg gctgaatact ggtttggtac
      181 ccaaccagga acttccgcct ctaccagcaa ccccggcaca ttgccaacat cgaatggtaa
      241 tcctgtgcct acttatgtac ggcattttga tggggtattt cttttgttcc tccagaatat
      301 tgttaacaac aggcaccagc ttatgctaaa gtatgactgg tatgatccca atatcaaggt
      361 caaagatgcc cagattggta agtcgggtac aaatctttca gctgcagata taaagttttc
      421 aactttaggt ataggatatg cttacctcat aaatccccaa accaagctta tcttatatta
      481 tgatattgta aagaacgaga agacacaact gactggctac actactgatt tgaaggataa
      541 tatactaacc tgcaggcttc agtttcgttt ctgagaaggg tgcttaacat tccggtaaca
      601 ttgccatcat aattggttaa catgggagta acataccggt aacgtaacgg gtacaccttt
      661 gcggcaaata actaagtatg tactccaggc tgcgattatt gttaagtgta ttgttcatta
      721 ccgcttttat ttccctgaat gcccaatctg ttaagctatc cgggaaggtg atgaacgaaa
      781 agaatgagcc actggctggt gccaatatta tcattaccaa tggcgcctcc aaacaagcca
      841 tttcagatgt agaaggtaaa ttctatttca cttttgaggc gggtgtaaaa catacattgg
      901 ttgtttcaag agttgggtat gcaacaaagg aaatcagtga agtggaagcg ttgttgaacc
      961 aggataatta cctggaaatt atactcaaag aaagttcaac aactaaactg gaaggtgtta
     1021 ccattacggc tacatcgagg agacaggaaa atacatctgc tcttttatct tttcaacgca
     1081 atagtacagc tctctccagc ggcctcgcgg cagattttat ccgtcgtacc cctgataaaa
     1141 acacaggaga ggttttgaaa agggtaagcg gtaccagtat ccaggatggt aaatttgtga
     1201 tcgttcgtgg attaagtgac cgctataatt ctgcagtgat caataatgcg caattgccca
     1261 gtacagagcc ggataaaaaa gcattctctt ttgatgtcat cccttctgcc ctgatagata
     1321 atatcattat caataaaacg gctacacccg aacttcccgg agaatttgcc ggaggtttga
     1381 tacagataaa taccaaagac gtacctacca ggaatttact gagtgtaggt gtaagctggg
     1441 gttttaatac gcaatcagtt tttaaagatt ttaccagcaa cgaacgtaac ggtaatgact
     1501 ggttgggttt tgatgacaga acaagaggat tgcccaaagg cttccctacc tcagcgcagg
     1561 cctacagagt attaggcgga acaacaaccg gaattcagca gcagatcgaa tacagcaagc
     1621 tgttcaataa tgatgtgtac aatgaaaaaa acagcacggc cttgccgacc caaacgtaca
     1681 atctgacctg gggtagcggt aaaaaattta aaaatggagg tacattaggt accatccttt
     1741 cgttgcagta ccgaaacagt atgctgaaat acgatgtgga gagaagattg catgaaaatg
     1801 acggggatgt gctggtacag ttatttgatg aacagaacag gtacagcgtc aatgtgggtg
     1861 cccttgccaa tatcacttat ataaaaggaa gacataagat ctctttcaag aatttattca
     1921 accaattgtt tgaagataat tattataccc ggggtggtgt aagtaatgac cgcatacagg
     1981 atattaattt ccgttcttcc gtattaaacc agcgttcttt atatagcggg caggtggaag
     2041 gtaatcacca actgaccaac agcgggatca aactgatgtg gaacggaaac tttgcctaca
     2101 actggaaatc acagccggac cttcgcactt ccgcttattt ccgtggaaaa ggaaccaatg
     2161 atccgtttga attcaacgat gacgataccc gtcgctttta cagtgatctg aaagattaca
     2221 gttacggtgc taatggcagc cttagtatac cctttacatt cgctaaacaa aaacaaacat
     2281 ttaaagccgg cggatccaca ttgatccgta tccgtgattt tcgcagccgt attttccgtt
     2341 atgaaccggc cagtatttcc cagtttgatg ccacaaaaaa cctgttgccc tacaaccaga
     2401 tatttgcacc tgaaaatatg accaccgatg gatttaagat ccttgatttt accaataacc
     2461 aggacaaata tttcggggtg tctatcctga atggcatgtt cggcatgttt gataataagt
     2521 ttggtgaaaa ggtaagattg gtatggggtg ccagagttga gaatttccag cagtttctga
     2581 ctacaaggga tgtaacagcc aaaagagtgg tggtgaatac agaaaaatgg gatgtgctgc
     2641 cttcattcaa cttcaccctt tctcccaata gcaaacacag catccgcata gccggcagtc
     2701 gtactgttgc caggccggag ttcagggaaa tagcgccgtt ctctttcttt gattatgaag
     2761 tgaattatgc tgttaacggt aatcctgacc tgaaaagaag ttctatactg aacggagata
     2821 tccggtatga gttttatccc aaaggtggtg aggctattac agtgggggcc ttctataaaa
     2881 attttgatga tccgatcgaa ttgcgtttga acccatccag tgtgctggac aggcgtaact
     2941 atgagtatac caatgccgat aaagctataa ctataggcgc tgagtttgaa gtaagaaaga
     3001 acctggattt tctgaatcaa tcattgacaa atttcagtgt gtttgccaac cttacttata
     3061 tctattcaaa agttacactg gcttctactt cttcatcagg tgctgctgtt tcatccaatc
     3121 gtccgttaca gggacaatca ccctacctgg taaaccttgg attgcaatat aacagcaaac
     3181 aaggtggatg gaacggctct ttattatata accgtattgg tcagcgcctg gcattagtag
     3241 gaataaatga tcttggtttc cctgatgtgt acgagaggcc aagggaccag gttgatatcc
     3301 agatcgctaa aaagatattt aataaccggg gagaacttaa gcttacctgg gctgatctgc
     3361 tgaatccggc ttactatttc tatgagaata cagacagcaa aaaagctttc agcagtggta
     3421 atgaccggtt gttctattct tataagcctg gttctactat ttcaattggt tttacctatg
     3481 actttaatgt tgggaaaaat aattcattcg ctaaaaaata aaaaaacaca aaacaaaaat
     3541 tatatgaaga agattctttt cggtgcactg gcttttatgg ctcttttcag tacaccctcc
     3601 tgtgtaaagg tgaattttga tgaaggagat gcgattactc ctcccgttga tcctaccagc
     3661 aatattatat ctggtgtgat ttcctccagt aaattttatg caaaaggaaa atggatactg
     3721 aaaggttatg tgtatgtaac tgaaggagcc actataactt ttgaagccgg ttgcgtggtg
     3781 cagagtgata ttaccgagaa aggtgctttg atcattgaaa gaggtgccaa gctgatcgct
     3841 tccggtacaa aagataaccc catcgtgttt accagtggta aagctgctgg ttcaagggca
     3901 cccggtgact ggggtggtat tatcttatta ggtaaggcgc caaccaatcg tccgcttgac
     3961 cccgctccaa caatcgaagg tggtgttggc cgccagtatg gcggaacaga tccactggat
     4021 gaaagtggta tcctgcgttt tgtacgtatt gagtttgcgg gcatagctgc cgagccagga
     4081 tctgagatca atggtttgac tatgggtggt gttggttccg gtactatcat cgagaatgtg
     4141 caggtgtctt ttggtaatga tgatgcgtat gaattttttg gcggaactgt aaatgccaaa
     4201 aacctgatcg cttttgcaac agcggatgat gactttgatt ttgattatgg gtatacagga
     4261 aagatccagt ttgccgtttc tatgcggaaa ccggattttg tagatgctgg tgatgccggt
     4321 aacggaattg agtgtgataa tgatggtagc ggtacaactg ctactccaat tacaaaacca
     4381 caattgagta atttcacttt tgttggtcct aatgacgcac cgggtacggc aggaaatcat
     4441 aatttttcaa accgctggag aagaagagct caatttattt tacgtaattc tattcttatc
     4501 ggacacccgg atgctggttt ctctatggaa tcagatggta cgttgaatga ttattatgta
     4561 aataatatct ctgaattcaa gaataacctt gtgcatgcaa ctgttttgcc ttataaaaca
     4621 gccaatgctg ctatagcaac taatgctcaa atacaggcta aagccgaaag cgaaggttgt
     4681 gttacattag ctacatcagc cgcagcacag ttaac
//
LOCUS       DAORTI010000200         4696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_22778_length_4696_cov_6.981685, whole genome shotgun sequence.
ACCESSION   DAORTI010000200 DAORTI010000000
VERSION     DAORTI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22778_length_4696_cov_6.981685"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLO70_15825"
     CDS             complement(<1..696)
                     /locus_tag="PLO70_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ75989.1"
                     /translation="MIQKIFLFIALSVSVLVSAQPEDVKKLHETAKAFMRSGDFDNAI
                     IVLTRALQQDKKNIDMQKDLVMSFYLKRDYAKAMEGAKVLIDRDDADVICYQIAGNVY
                     KALEEVKECDKMYKKALKKFPKSGPLYSEYGELLWATKDFAAIKQWEEGIKSDPGYGG
                     NYYNAALYYFYTKDKVWSLIYGEIFVNMESFSERGAAMKQMLLQGYKEKLFSDPDIMK
                     GEEKNKSEFATAFL"
     gene            complement(773..1993)
                     /locus_tag="PLO70_15830"
     CDS             complement(773..1993)
                     /locus_tag="PLO70_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQZ75990.1"
                     /translation="MAIIKPFTALRPQQQLSQQVASRPYDVLNSEEAKTEAAGNPFSF
                     LHVTKSEIDLPAGIDIHSQEVYDKAKENLQQFIANGILFKEEKPSYYIYQLVMKGRSQ
                     TGLVCVSSVDDYFKNTIKKHEFTRPEKEKDRIEHMTTIRAQTGNVFLAYRDVETVNTI
                     IHNWKYTYQPVYNFTATDGIQHAIWVVNDQSVIDDITSQFQVNVPFTYIADGHHRAAS
                     AAKVSKSLPDSNEAKYFLTTIFPASQLAILDYNRVVKDLNGLSASKFLGFLQDDFYIT
                     HSPEPVKPAQLHEFGLYLDHKWYILTARKGTWTDDPIGVLDVTILSNNILDKILDIKD
                     QRTDKRIDFIGGIRGLKELEKRVDSDEMKAAFSLYPVTIDQLFTIADSGNVMPPKSTW
                     FEPKLRDGLLTHLI"
     gene            complement(2000..3070)
                     /gene="serC"
                     /locus_tag="PLO70_15835"
     CDS             complement(2000..3070)
                     /gene="serC"
                     /locus_tag="PLO70_15835"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQZ75991.1"
                     /translation="MVHNFNAGPSILPKEVFEQASKAILDFNNTGLSILEIGHRTPLF
                     QPVMDEARALVKELMQLDADHEVLFLHGGASTQFMQIPMNLLNENDIASYTETGTWAN
                     KAIREAKLFGHVEIAGTSKDAAYTSIPNYLTVPPTSAYLHITTNETIAGTQWHTIPYD
                     CGVPLVADMSSDILSRQLEFNKFDLIYAGAQKNMGAAGVNLVVVNKNILGKVNRTIPT
                     ILDYRNHIAEGSMLNTPPVFAVYVVMLTLQWLKDQGGVTTIEKKNNEKATLFYNTLDA
                     LPVFKGPVAKEDRSKMNAVFVMDNPGLEKEFQEICKKEGMVGIKGHRSVGGFRISMYN
                     ALTIDSVKALTDLMKEFATKKG"
     gene            complement(3149..4114)
                     /locus_tag="PLO70_15840"
     CDS             complement(3149..4114)
                     /locus_tag="PLO70_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HQZ75992.1"
                     /translation="MKKIITSFLFLSLSLNCFCWGFYAHRKINHYAVFLLPPEMMVLY
                     KPHIDFLAEHAVDPDKRRYAVPEEGPRHYIDIDHYGNYPYDALPRKWSDAVAKYSEDT
                     LNQYGIVPWWLQTMLFRLTDAFKEKNQAKILKSSAEIGHYISDSHVPLHACSNHNGQY
                     SDQKGIHGFWESRIPELLAEKEWDFFIGKAEYIKNPADFIWTRVLESAAAADTVLQFE
                     KQLTKKYPGDQKFAYEDRNGTIIRQYSSSFSIAYNNQMKGMVERRMRQSIYAVASFWY
                     TAWVNAGQPNLTQLSNREFSPEDLKEFESLNQSWKNSAIKGREHE"
     gene            complement(4120..>4696)
                     /locus_tag="PLO70_15845"
     CDS             complement(4120..>4696)
                     /locus_tag="PLO70_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQZ75993.1"
                     /translation="FFQGLIILAPIGITLYAMYWLFERVDGILRPYVNIPGLGFLLII
                     LFVILVGWISSNFLMGSAITFFDQLMEKTPGVKLIYTSTKDFFEAFAGDKRKFNRAVL
                     ANVFSEDVWIVGFLTDEEMQKFELGADKVAVYVPQAYNFAGQLYILPKEKVRKIEKLT
                     SGEAMKYAVTGGVVDLDAERKEEVEKKLKQD"
BASE COUNT         1269 a          991 c          923 g         1513 t
ORIGIN      
        1 caaaaatgct gttgcaaact cactcttatt tttttcttcc cctttcataa tatcaggatc
       61 tgaaaataat ttttccttat agccctgtaa aagcatttgt ttcatagcag cccctctttc
      121 gctgaagctt tccatattta caaaaatttc tccataaata aggctccaca ctttgtcttt
      181 cgtatagaaa taatataaag cagcattata atagttgcca ccatagcccg ggtcggattt
      241 aataccttct tcccattgtt taatggcagc gaaatcttta gtcgcccaga gcaactcacc
      301 atattcactg tatagcggcc cgcttttagg aaatttcttc aatgcctttt tatacatttt
      361 atcgcattcc tttacttctt cgagtgcttt ataaacattg ccggctatct ggtaacaaat
      421 cacatccgca tcatcccggt caatcaaaac ttttgcgcct tccattgctt ttgcataatc
      481 ccgtttcagg taaaagctca tgacaagatc tttttgcata tcaatatttt tcttatcctg
      541 ctgcagagcc cgggttaata cgatgattgc attatcaaaa tcaccactac gcataaaggc
      601 ttttgcagtc tcgtgtaatt ttttgacatc ttcaggttga gcagatacaa gcactgaaac
      661 ggataaggcg ataaagagga atattttttg gatcatagcg taaattaaaa aagaagagac
      721 gcaattatga tcgtctctgt tgcaaaataa caaatatttg agggcttaac tgttaaataa
      781 ggtgtgtaag caggccatcc ctcagttttg gttcaaacca ggtgcttttg ggcggcatta
      841 cattgccgct atctgcaata gtaaataact ggtcaatggt aaccgggtat aagctgaatg
      901 ccgctttcat ttcgtcacta tccactcttt tttccaattc ttttaatccc cttataccgc
      961 ctataaaatc aattcttttg tctgtacgct ggtcttttat atcaaggatc ttatcgagaa
     1021 tgttattaga aagaatggtt acgtccagca ccccgatggg gtcatcggtc caggttcctt
     1081 ttcttgcagt gaggatatac catttgtgat caaggtataa accgaactca tgtaattgtg
     1141 cgggtttaac cggttcaggg ctgtgtgtta tataaaaatc atcctgtaaa aagcccagga
     1201 attttgatgc cgataatcca ttcagatctt ttactacacg gttataatcc agaatagcta
     1261 attggctggc cgggaaaatt gtagtgagaa aatactttgc ttcattgctg tccggcaacg
     1321 atttgctcac tttggctgct gatgctgccc ggtggtggcc atcggctata taggtaaaag
     1381 gcacattaac ctggaattgt gaagtaatat catcaataac tgactggtca ttcactaccc
     1441 aaatagcatg ctgtatacca tctgtggcag taaaattata tacaggctga taagtatact
     1501 tccagttatg aataatagta tttacagttt caacatcacg gtaggcgagg aatacatttc
     1561 ctgtttgtgc ccggatagta gtcatgtgtt caatcctgtc tttttctttt tcaggccggg
     1621 tgaactcatg ttttttgatg gtgtttttaa aataatcatc tacggaagac acgcagacca
     1681 aacctgtctg gcttcggcct ttcatcacca gttgatagat ataataagat ggtttttctt
     1741 ctttgaacag aatgccattg gcaatgaact gctgtaaatt ttctttggct ttatcatata
     1801 cttcctggct atgaatatca atgccggctg gcaaatcaat ttcagatttg gtgacatgaa
     1861 gaaaagaaaa tggattgcct gcagcttcag tttttgcttc ttcgctgttc agcacatcat
     1921 agggtcttga tgcaacctgt tgtgataatt gctgctgggg tcttaatgca gtaaagggtt
     1981 taataatagc catctcggtt tatccttttt ttgttgcgaa ttctttcata aggtcagtca
     2041 gtgcttttac actatcaatg gtcaatgcat tatacattga gatacgaaaa ccaccaacac
     2101 tacgatgtcc ctttattccc accattcctt cttttttaca tatctcctgg aattcttttt
     2161 ccagtcctgg gttatccatt acgaagacag cgttcatttt actgcggtcc tctttggcca
     2221 caggcccttt aaaaacaggt aaagcatcta aagtgttgta aaataaggtt gctttttcgt
     2281 tgttcttttt ttctatggta gtaacgcctc cctgatcttt tagccattgt aatgtgagca
     2341 tcacaacata tacggcaaaa acaggtggcg tattcagcat actaccttca gctatatgat
     2401 tgcgatagtc aagaatagtt ggaattgttc tgttaacttt tcccagaata tttttattga
     2461 ccacgaccag gtttacacca gcagccccca tgttcttttg tgcacctgca tagataaggt
     2521 caaacttgtt gaattcaagc tggcggctga gtatatcact gctcatgtcg gctaccagtg
     2581 gtacgccaca atcgtaggga atagtatgcc actgtgtacc ggctattgtt tcattagtcg
     2641 taatgtgcag gtatgcagat gtgggtggaa cagtaagata gttgggaatg cttgtataag
     2701 cagcgtcttt tgaggtgcct gctatttcca catggccaaa taattttgct tccctgatcg
     2761 ctttattagc ccaggtgccg gtctctgtat aggaagcaat atcgttttcg ttcaggaggt
     2821 tcatgggaat ctgcatgaac tgggtggaag ccccgccgtg caggaacaac acttcatgat
     2881 ctgcatccag ctgcatcaat tcttttacca atgctcttgc ttcatccatt acgggttgaa
     2941 acaaaggtgt acggtgacca atctctaaaa tagataaacc ggtattattg aaatcaagga
     3001 tagctttact ggcttgttca aaaacctctt tgggaaggat ggaggggcct gcgttaaaat
     3061 tatgaaccac ttattacaaa tctttaatgt tgctgataat tgaaggcgca aagatacttt
     3121 ttagcaggta ttggaaacgt aatatttatc actcatgttc ccggccttta atagcgctgt
     3181 ttttccatga ttgatttagg ctttcaaatt ctttcaggtc ttccggggaa aactccctgt
     3241 tgctgagctg agtaagattg ggctgaccgg catttaccca ggccgtgtac caaaatgagg
     3301 cgactgcata aatggactgt cgcattcgcc tttccaccat tcctttcatc tggttattat
     3361 aagcgataga aaatgaagac gaatactgac gaatgatagt tccgttacgg tcttcatatg
     3421 caaatttctg atcaccggga tattttttgg tcagttgctt ttcaaattga agtacagtat
     3481 cagcagcggc tgcactttcc aaaactctgg tccatataaa gtcggccggg ttttttatgt
     3541 attctgcttt cccgataaag aagtcccatt ctttttctgc cagcagctca gggatacggc
     3601 tttcccaaaa accatgaatc cctttctgat cgctgtattg cccgttatgg ttgctgcagg
     3661 catgcaatgg tacatgcgaa tcggaaatat aatggcctat ttctgctgag gatttaagga
     3721 tctttgcctg gttcttctct ttaaaggcat cggtcagcct gaagagcatg gtttgcagcc
     3781 accagggtac tataccatat tgattcaggg tgtcttctga atactttgcc actgcatcac
     3841 tccatttcct tggtaatgca tcatacggat agttaccgta gtgatcaata tctatataat
     3901 gcctggggcc ttcctcgggt actgcatatc ttcttttatc agggtctaca gcatgttctg
     3961 ccaggaaatc aatatgaggt ttgtacaaga ccatcatttc cggtggtaat aagaacactg
     4021 cataatgatt gatctttcgg tgggcataaa aaccccagca aaagcagttg agtgatagtg
     4081 aaaggaaaag aaatgatgta attatttttt tcatcattat taatcctgtt taagcttttt
     4141 ctcaacttct tcttttcgtt ccgcatcaag gtctaccaca ccaccggtca cggcatactt
     4201 catggcttcc ccggaagtta atttttctat ttttctaact ttctcctttg gtaagatgta
     4261 caattggccg gcaaaattgt aagcttgtgg cacatacaca gccactttgt ccgcaccgag
     4321 ttcaaatttt tgcatttctt catccgtcag gaatcctacg atccacacat cttctgagaa
     4381 cacattggcc agcaccgccc ggttgaattt tcgtttgtca ccggcaaaag cttcaaaaaa
     4441 atctttggtt gaggtgtaga ttaatttaac tcccggtgtt ttttccatca gttggtcaaa
     4501 aaaggtgata gcactgccca tcaggaaatt ggaacttatc cagcctacaa ggatcacaaa
     4561 taatatgatc agaagaaagc ccaggccagg aatgtttaca taaggccgta aaatgccgtc
     4621 caccctttca aacagccaat acatggcata gagagtaata ccaatgggtg ccaggatgat
     4681 taaaccttga aaaaaa
//
LOCUS       DAORTI010000201         4660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23059_length_4660_cov_6.036482, whole genome shotgun sequence.
ACCESSION   DAORTI010000201 DAORTI010000000
VERSION     DAORTI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23059_length_4660_cov_6.036482"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLO70_15850"
     CDS             complement(<1..837)
                     /locus_tag="PLO70_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQZ75994.1"
                     /translation="MTSGKINILMTGGGAPGAAGILKCLKLEPAFTIIVADANSNAVG
                     KYLAKNFITIPRADDPSFIDVLLSVCREKNIHAVLPLVTKELIRLSQHSKEFELAGTK
                     LLISPAASLEIANNKSRLYEFLQWRGIPVPGFRIVETIEQFKSAVNELGYPSKQICFK
                     PSVSNGSRGFRIITDQINELDLLFNYKPNSTYISYNDAVRVLSSGSFPELLVSEYLPG
                     EEYSVDCLANQGESVLVIPRLRKKMINGISVEGEFVNNEAIFSYCKQIIKELQLHSNI
                     GIQ"
     gene            complement(858..2774)
                     /locus_tag="PLO70_15855"
     CDS             complement(858..2774)
                     /locus_tag="PLO70_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HQZ75995.1"
                     /translation="MAWKNQQQFIEALENAGELLRIKTFVDPKLEIAEITDRISKSGN
                     GGKALLFENTGYEFPVLMNAYGSEKRMSMALGVEQLDDVAREIESLFHLLSSPKEGIL
                     DKLKLLPKLGQFASWMPKVKKGRGECQEVVMENPDITKLPVITCWPKDGGPFVTLPVI
                     HTKDPNTNSRNVGMYRMQVFGPALTGMHWHKHKVSAKHFTEYKKLNQKMPVAVALGGD
                     PVYAYAATAPLPENVDEYMLAGFLRKKKVELVKCISQPEVEVPADADFIIEGYVDPND
                     ELIWEGPFGDHTGYYSLPDWYPKFHITAITHKKNAVYPATIVGIPPQEDAWLGKATER
                     IFLAPIKMTMVPEITDMDMPIEGVFHNLVITKIKKDYAGQGQKVMNAMWGAGQMMFNK
                     ILVLADEGVKIQDYEALAKYVFKNLNPATDIYFSTGPMDVLDHSCSKLGFGGKMCIDG
                     TAKMEEEMDDNYEAGVLSQEPAAIAVNLKNRFPEIRSVNVSLLEKEIPVLIIAVEKNR
                     AGHIKQLHEQVAPMPEVQGLKMILYVEHTVDANDLPTALWRFCNNLDPKRDFHLYKNA
                     VNRYTAYMGLDGTRKTKAYDNFHRDWPNIIVADDATIKSVDEKWNALGIGGFIPSPSL
                     KFKDQLYGEEAVVN"
     gene            complement(2844..3707)
                     /locus_tag="PLO70_15860"
     CDS             complement(2844..3707)
                     /locus_tag="PLO70_15860"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQZ75996.1"
                     /translation="MKKVHLFILFCLLTTTVLSQKVHIGLFGGLSAYNGDLVDKIFPK
                     KVTNGAIGITGSYELTDQIMLRAGLTYSIVGGADRYSSDTVLRLRNLSFETSIVEFSA
                     VGEYYLFNLYERKYSPYIFGGLAIYKFNPYTYDGSNQKIFLKPLSTEGQGLAGYPGSK
                     PYTLTQMALPFGGGVKFAVNDNLRIGVEAGLRKLFTDHLDDVSGNYVDQADLLAAKGQ
                     LAVDLSYRGDEVAGGSLVYPAKGAQRGGAKFNDIYYFTGIHLTYRLGGSNGGGFGGGK
                     KGRNGCPVNVY"
     gene            complement(3814..>4660)
                     /locus_tag="PLO70_15865"
     CDS             complement(3814..>4660)
                     /locus_tag="PLO70_15865"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQZ75997.1"
                     /translation="YGISNAQEFDKTASFGFFTGVINYQGDLNPNSFTLKHSNFATGI
                     FARKPMNRWLTLRAGITIGKIEASDSWNRDYLKSRNLSFTTSVKEAYAGFELTILDMS
                     AKRFTPYIYGGIAVFHFNPWTYDNTGMKTFLKPLSTEGQGLAEYPEQKPYNLTQFALP
                     FGIGARFAVTDDINIGIEFNQRKSFTDYIDDVSTHYVDRDVLLQAKGTKAVELAYRGG
                     QSPLGQPQYPQHGEQRGTPSEMDWYYFLGLTMEFKLNAFSSLFGGGINKKGIASQRCP
                     RNITY"
BASE COUNT         1300 a         1044 c          890 g         1426 t
ORIGIN      
        1 ctgtatgcca atattactgt gtaactgcag ctcttttatt atttgtttgc aataggaaaa
       61 gattgcttca ttattgacaa attctccttc tacagagatc ccgttgatca ttttttttcg
      121 caaccgtgga atgaccagaa cagattcacc ctggtttgcc aggcaatcca ccgaatattc
      181 ctcaccgggc aaatattcgc ttaccaataa ttcagggaat gagccggaag atagaaccct
      241 gacagcatca ttgtagctta tataagtgga attgggttta taattaaata atagatccag
      301 ttcattgatc tggtctgtta ttatcctgaa tccccggctg ccattagaaa cagaaggttt
      361 gaaacaaatt tgttttgacg ggtaccccaa ctcattcaca gcacttttaa attgttctat
      421 cgtttctaca attctgaaac cgggaactgg tattcctctc cattgtaaaa attcataaag
      481 gcggctttta ttattagcta tctccagtga tgcagccggt gagatcagca gtttggtacc
      541 tgccagttca aattctttgc tatgctgtga cagccggatt aattcttttg ttaccagcgg
      601 caataccgca tgaatatttt tttcccggca aacagaaagg aggacatcta tgaaagaagg
      661 atcatctgcc ctgggaatag taataaaatt ttttgcgagg tatttgccaa ctgcattact
      721 gttggcatct gctacaataa tagtaaaggc aggctccagt ttaagacact ttaagatacc
      781 cgctgcaccc ggagcaccgc cgccagtcat gagaatatta atttttccgg atgtcacagg
      841 ttaaatgtag atgaatttta gtttacgaca gcttcctcac catatagctg gtctttgaat
      901 ttaagtgaag gggaaggtat aaaaccgcct attcctaatg cattccattt ttcatccact
      961 gattttatcg tggcatcatc tgccacaata atattgggcc agtcgcggtg gaaattgtca
     1021 tacgctttcg tctttcttgt accgtcaagg cccatgtaag ccgtataacg gttcactgca
     1081 tttttataaa gatggaaatc tcttttgggg tcgaggttat tgcaaaaacg ccagagggct
     1141 gttggcagat cattcgcatc aactgtatgc tccacgtata aaatcatttt tagtccctgt
     1201 acttccggca taggggctac ctgttcatgc aactgtttga tatgtcctgc cctgttcttt
     1261 tcaacagcta taataagtac aggaatttct ttttccaata aagaaacatt gacggatctg
     1321 atttcgggga acctgttttt caggttaact gctatggcag ctggttcctg gctcaaaact
     1381 ccggcctcat aattatcatc catttcttct tccatcttcg ccgttccatc gatacacatt
     1441 tttcctccaa aacccagttt gctgcaacta tgatccagta catccatcgg ccccgtggag
     1501 aaataaatat cggtagcggg gttcaggttt ttgaacacat atttagccag cgcttcatag
     1561 tcctggatct tcactccctc atcagccagc actaatattt tattgaacat catttgaccg
     1621 gcaccccaca tggcattcat cactttttgt ccctggcctg catagtcttt tttaattttt
     1681 gtaataacaa ggttatgaaa cactccttct ataggcatgt ccatatctgt gatctcaggt
     1741 accattgtca tttttatagg cgccaggaaa attctttcgg tagcttttcc caaccaggca
     1801 tcttcctgtg gcggaatacc aacaattgta gccggataaa ctgcattttt cttatgcgta
     1861 atagccgtga tatgaaactt aggataccag tcgggcaatg aataataacc ggtatggtca
     1921 ccaaacggcc cttcccagat gagctcatcg ttcgggtcaa cataaccttc aataataaaa
     1981 tctgcatcgg caggcacttc tacttctggt tgtgagatac attttaccag ctctactttt
     2041 ttcttacgta aaaaaccagc gagcatatat tcatccacat tctccggcag tggagcagtg
     2101 gcagcataag cgtatacagg atcaccaccc agcgcaacag ccaccggcat tttttgattc
     2161 agctttttat actcagtaaa atgtttggca ctcactttat gtttatgcca gtgcatacct
     2221 gtcaaagcag gaccaaaaac ctgcatacgg tacatcccta cattgcgtga atttgtattc
     2281 ggatctttgg tatgaattac cgggagtgta acaaaagggc cgccatcttt tggccagcag
     2341 gtgataacgg ggagtttggt gatatcagga ttctccataa ctacttcctg gcattctcct
     2401 ctcccttttt taacctttgg catccaactg gcaaactggc ccagtttagg cagtaatttt
     2461 aatttatcaa ggattccttc tttaggcgaa gacagcaaat gaaaaagaga ttcaatttct
     2521 ctggccacgt catcaagctg ttccacaccc agggccatcg acattctctt ttcacttcca
     2581 tacgcattca tcaatacagg aaattcatat cctgtatttt caaacagtaa tgccttaccg
     2641 ccgttgcctg atttgctgat acggtcagtt atttccgcaa tctcgagttt aggatcgaca
     2701 aatgttttga tgcggagcag ttctccggca ttctctaaag cttcaataaa ttgttgttga
     2761 tttttccagg ccatgttgta gtataaaatg ttgcgaaggt acaacactac ctgtccataa
     2821 ttgttcagaa tcaggaatga atatcaatag acattaacgg gacaaccatt gcggcctttt
     2881 ttcccgccac cgaacccacc tccgttactt ccacctaatc tgtatgtgag atgaataccg
     2941 gtgaaataat agatatcatt gaatttagcg ccgcctcgct gggcaccttt tgcaggatag
     3001 accaggctgc ctccggccac ttcatcgccc cgataggata aatcaacggc taactgaccc
     3061 tttgctgcga gcaggtccgc ctgatcaaca taattaccac taacatcatc aaggtgatcg
     3121 gtgaagagtt tccttagccc tgcttcaact cctattctca aattatcgtt aactgcaaac
     3181 ttcacacctc cgccaaaagg taaagccatt tgtgtaagcg tataaggttt acttcccgga
     3241 tagcctgcca aaccctgccc ttctgtactt aagggtttaa gaaatatttt ttgattgctg
     3301 ccatcataag tatagggatt gaacttataa atagccagtc ccccaaaaat ataaggggag
     3361 tattttcttt cgtataaatt aaataaataa tattcaccta cggcgctgaa ttcaacaatg
     3421 gaagtttcaa aagaaagatt cctgagtctt aatacagtat cactgctata cctgtctgcc
     3481 ccgcctacaa tactgtaagt aagtcctgct cttaacatga tctgatcagt aagttcataa
     3541 ctaccggtga tgccaatggc tccattggtt acttttttag gaaatatctt gtcgaccagg
     3601 tcaccattat acgcagatag acctccgaat aaaccaatat gtaccttttg agaaaggaca
     3661 gttgttgtca aaagacaaaa aagaatgaaa aggtggactt tcttcataac ctgattattc
     3721 tgtctgtcag caaattttag gccgatataa gcctctgacc agtaataaac ggcaaagatt
     3781 agtttgtagt gtactatttt ggaataaaat gggtcaatag gtaatattcc ggggacagcg
     3841 ctggctggca ataccctttt tattgatacc tccgccgaaa agactgctga aagcattgag
     3901 tttaaactcc atggttaacc cgagaaaata ataccaatcc atttcagaag gagttcctct
     3961 ttgttcgcca tgttgaggat attgaggttg gcccaaagga gactggccgc cccggtaagc
     4021 taattcaaca gcttttgttc cctttgcctg taacaacaca tcccggtcta cataatgagt
     4081 acttacatca tcaatataat cagtaaagga ttttctttga ttaaattcaa ttccaatgtt
     4141 aatatcatcc gtaaccgcaa atcgggcacc tattccaaat ggtagtgcaa actgtgttag
     4201 attataaggt ttctgttcgg ggtattcagc aaggccctgc ccttctgtac ttaaaggctt
     4261 aaggaatgtt ttcatacctg tattatcata tgtccatgga ttaaaatgaa aaacagctat
     4321 accgccatag atatagggag tgaatctttt tgcagacata tccagtatgg tcaattcaaa
     4381 accggcataa gcttctttta cagaagtggt gaaacttaaa ttccgtgatt tcaggtaatc
     4441 cctgttccag ctgtctgatg cttcaatttt gccgattgtt attccagctc ttagggtaag
     4501 ccagcgattc atgggcttac gggcaaaaat gcctgtagca aaatttgaat gctttagggt
     4561 gaaactattg ggattcaggt ccccctggta gtttattacc ccggtaaaaa agccaaatga
     4621 tgctgtttta tcaaattcct gtgcatttga tatgccatac
//
LOCUS       DAORTI010000202         4645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23167_length_4645_cov_4.329194, whole genome shotgun sequence.
ACCESSION   DAORTI010000202 DAORTI010000000
VERSION     DAORTI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23167_length_4645_cov_4.329194"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLO70_15870"
     CDS             complement(<1..208)
                     /locus_tag="PLO70_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQZ75998.1"
                     /translation="MSYKKFSLMLLISFMIMYAVMFLNITQVDYFYVSTTRIYMALLM
                     ISPMAVVMMLMMGKMYPNKKLNTGI"
     gene            complement(219..2285)
                     /locus_tag="PLO70_15875"
     CDS             complement(219..2285)
                     /locus_tag="PLO70_15875"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HQZ75999.1"
                     /translation="MDHNKHEHHQQQEMDHGNMDHSKMHHDHGSIPMGMAGHDHHKMM
                     IADFKKRFWVSLILTIPVLILSPMIQGFFGYSLSVPENPYVLMALSSVIYFWGGWPFL
                     KGFITEIKQKGPGMMTLIAMAISVAYFYSTATVFGLKGMDFFWELATLIVIMLLGHWL
                     EMKSVLGASKALQLLVSMMPAEAHRVKSDGQVEDIKLEALKKEDIILIKPGEKVPADG
                     IIVEGSSYLNESMLTGESKPVKKEKENKVIGGSINGNGSLKIKVEHTGKDSYLNKVIK
                     LVEEAQKAKSKMQNFSDRAAKWLTYIALSVGFGTLAVWLIAGYPFVFALERMVTVMVI
                     ACPHALGLAIPLVVAISTAVSAQNGLLIRNRTAFEESRKITALLFDKTGTLTTGVFGV
                     TRFVSVNTEFSKDEILRLASALEQTSEHPIAVGIINKVKEHNVAIPKAEKFNAITGKG
                     VEAMVEGKSVKVVSPGYLKDKNLTIPEGAYSSKAETVVFVLVDDKLTGYIALADAIRP
                     ESAEAIQVFKKNGIKVLMATGDNETVAKAVSDELKLDGYYSEVLPHQKVEIVKELQSK
                     GEFVAMTGDGVNDAPALAQSDVGIAVGSGTDVAAETADIILVNSNPKDIANLILFGKA
                     TYKKMVQNLVWATAYNSFAIPLAAGVLYNAGFVLGPAVGAIFMSLSTIVVAVNAQLLK
                     TKLNKS"
     gene            complement(2297..2836)
                     /locus_tag="PLO70_15880"
     CDS             complement(2297..2836)
                     /locus_tag="PLO70_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQZ76000.1"
                     /translation="MEKQNKTTNWFVITGGPSTGKTTVINLLTERGYKTTIEHARHYI
                     DTMKEEGRTVEEIRANKRKFQLGVLDMQIEQEAELSPSDTVFLDRAIPDAMAYYRFLK
                     LEYDKILLDAMKEVAYKKIFILDRLPFVNDYARTEDEQAQKLIHQLIIEVYQSLGFPV
                     VFVPVISPDERVEFILKNL"
     gene            complement(2848..3324)
                     /locus_tag="PLO70_15885"
     CDS             complement(2848..3324)
                     /locus_tag="PLO70_15885"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HQZ76001.1"
                     /translation="MKYLPKSKWKKAGLILLLIFVLIQFYPRPEKNISSAQSSNSIEQ
                     LYPMQDSILQVLKAACYDCHSNNTNYPWYSTIQPVAWFLNRHIVEGKEELNFDEFGTY
                     SKRRQQSKLKAIVNQVKDGEMPLASYKLLHKKARLSGKERSMITKWFMEKYDASRN"
     gene            complement(3334..4545)
                     /locus_tag="PLO70_15890"
     CDS             complement(3334..4545)
                     /locus_tag="PLO70_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQZ76002.1"
                     /translation="MKKYLIIIASVLFVLASCKSKKTTTVNKDEYYTCSMHPQIMQDK
                     PGTCPICHMDLIVVKKTNTAADEILLNDEQIRLGNIQTDTIRNGMIGDKMILTATLNT
                     DETKVNSINARIMGRIDRLYFKNMGDYVSKGAHLYDLYSEELNNAKQEYIVAVEKQQS
                     LDNSIIDFNRLVQSAKTKLLLWGMSEAQVNELTKTKKASPLTSFYSNESGYITELPVM
                     EGQYVSEGSIIVKLADLSSLWAEAQVYTSQLSAIDLKGTAIVQFPDIPGKEWKGKIEF
                     ANPEIVSDSRLNLVRVSIPNPGGLLKPGMPAYVSIKSKELNTLTLPSDAVLRDGKMNI
                     VWVQSGKNSYKMKMVQTGLESGDRLEIKSGLQNGDVVVTRGAYLLNSEFVFKKGGNTM
                     AGMEGMDMSSH"
BASE COUNT         1237 a         1025 c          767 g         1616 t
ORIGIN      
        1 taataccggt attcagtttt ttgttagggt acattttccc catcatcagc atcatcacta
       61 ctgccattgg agaaatcatc agcagggcca tataaatacg ggtagtactt acataaaaat
      121 aatcaacctg ggtaatattt aaaaacataa ctgcatacat tatcataaat gatatcagca
      181 acattaaaga gaattttttg taactcataa ttatcgtttt atgatttatt taatttagtt
      241 tttaataact gtgcatttac tgcaacaaca attgtgctta aactcataaa aatcgctcca
      301 acagccgggc ctaatacaaa cccagcatta taaagtacgc ctgctgctaa tggaatggca
      361 aagctgttat aggcagttgc ccaaaccagg ttttgtacca ttttcttgta cgtggctttt
      421 ccaaatagaa ttaaattagc aatgtccttt ggattactat taactaaaat aatatcggct
      481 gtttctgctg ctacatctgt accagatccc actgctattc caacatctga ttgcgccaaa
      541 gccggtgcat catttacacc atcacctgtc atagcaacaa attcaccttt gctttgcagt
      601 tcttttacaa tctccacttt ctggtgcggt aacacttctg aatagtatcc atccaatttc
      661 aattcatcag atacggcttt ggccaccgtt tcattatctc cggttgccat caatacttta
      721 attccattct ttttaaacac ctggattgct tctgcacttt cgggccttat cgcatctgcc
      781 aatgcaatgt atcctgtcaa cttatcatca actaaaacaa aaacaacagt ttctgctttg
      841 cttgaataag ctccttcagg aatggtcaga tttttatctt tcagatatcc ggggcttacc
      901 acttttactg atttcccttc taccattgct tcaacacctt tgcctgtaat ggcattaaat
      961 ttttcagctt tgggaatggc aacgttgtgt tcttttactt tattgattat accgacagca
     1021 ataggatgct ctgatgtttg ttccaaagca ctcgccaacc tcaatatttc atcttttgaa
     1081 aattcagtat taactgacac aaatctggta acaccaaaaa cgccggttgt taatgttcct
     1141 gtcttgtcaa acaggagtgc tgtaattttt cttgattctt caaaagccgt tctgttacga
     1201 attaataaac cgttttgtgc tgatactgct gttgaaatgg ctactaccag gggaatagcc
     1261 aaacccaatg catggggaca ggcaattacc attaccgtta ccatacgctc taatgcaaat
     1321 acaaatggat atcctgctat cagccaaaca gccaatgttc caaaacctac cgataaagca
     1381 atataagtaa gccatttggc agccctgtcg ctgaagttct gcattttgga ttttgctttc
     1441 tgtgcttctt ctaccagctt aatcactttg ttcaggtaac tgtctttccc tgtatgttcc
     1501 acttttatct tcaaagagcc gttaccattt atagagcctc caatcacttt attttctttc
     1561 tctttcttta ctggttttga ttctccggtg agcatagatt cgtttaagta actgctgcct
     1621 tctacaataa ttccatcagc aggtactttt tctccgggct tgatcagtat aatgtcttcc
     1681 ttctttaatg cttccagttt tatatcttcc acctgaccat ctgactttac ccggtgtgct
     1741 tcggcaggca tcatgctaac taataattgc agggctttgg atgcccctaa tacagatttc
     1801 atttcaagcc aatggcctaa gagcataatc acaatcaatg tagccagttc ccagaaaaaa
     1861 tccattcctt ttaatccgaa aacagttgct gtgctgtaaa aataggcgac agaaatagcc
     1921 attgcaatca gggtcatcat gcccggacct ttctgtttaa tttcggtaat gaaacctttc
     1981 aggaaaggcc agccgcccca gaaatagatt acagatgaaa gcgccattaa tacataggga
     2041 ttttctggca ctgataagct atatccaaaa aaaccttgta tcatcggtga taatatcaat
     2101 acgggtatag taagaataag gcttacccaa aaccgttttt taaaatcggc tatcatcatt
     2161 ttgtgatggt cgtggcctgc catacccatc ggtatgctgc cgtggtcgtg gtgcatttta
     2221 ctgtgatcca tattaccgtg atccatttcc tgctgttgat gatgttcgtg tttgttatga
     2281 tccatgataa tttattttac aggttcttta atataaattc aactctttca tcggggctga
     2341 ttaccggtac aaaaacaact ggaaatccta atgactgata tacttctata attaactggt
     2401 gaattagttt ttgtgcctgt tcatcttctg ttcttgcata atcatttaca aaaggcagcc
     2461 tgtcaagaat aaatattttt ttataagcga cttccttcat tgcatcaagt aatattttat
     2521 catattcgag tttcaaaaat cggtaataag ccattgcatc ggggatagcc cggtcaagaa
     2581 aaacagtatc ggatggcgac aattcggcct cctgttcaat ctgcatatct aataccccta
     2641 actgaaattt tcttttattt gccctgatct cttcaacagt tctcccttcc tctttcattg
     2701 tgtctatata atgccttgca tgttcaatag tggttttata acctctttcc gttagcaggt
     2761 ttatgacagt tgttttccca gtgctgggcc caccggtaat gacaaaccaa tttgtcgttt
     2821 tattttgttt ctccatgttg tgtcaattca atttctactg gcatcatatt tttccatgaa
     2881 ccattttgtt atcatactgc gttctttgcc agacagtctt gcctttttat gcagcagttt
     2941 ataagaagcc aaaggcattt ccccatcttt aacctgattg actatagcct tcaacttact
     3001 ttgctgcctt ctttttgaat aagttccaaa ctcatcaaaa ttcaattctt ccttaccttc
     3061 cactatatgc ctgttcagaa accatgcaac aggctgtatg gtactatacc aggggtagtt
     3121 tgtattgttg ctgtggcaat cataacatgc tgcttttaaa acctgtaata tagaatcctg
     3181 catgggatat agttgttcaa tcgaattact gctttgtgca gatgaaatat ttttttccgg
     3241 tcttggatag aattgaatca aaacaaatat cagcagcaat atcaatcctg cttttttcca
     3301 ttttgatttg ggtaaatatt tcatttttct ttcttaatgt gaactcatat ccatgccttc
     3361 cattcctgcc attgtattac cgcctttctt aaatacaaat tcgctgttta ataaatatgc
     3421 gcctctcgta accaccacat cgccattttg taaccctgat tttatttcca gcctgtcgcc
     3481 gctttccaaa ccagtttgta ccattttcat cttataactg tttttgccgg actgtaccca
     3541 tacaatattc attttaccgt cacgcagtac tgcatctgat ggaagtgtaa gtgtattcag
     3601 ttctttactt ttaatgctga cataagccgg cattccgggt ttaagcaatc caccgggatt
     3661 aggtatggaa acacggacca ggttcagcct gctgtctgaa acaatttccg gattggcaaa
     3721 ttctattttc cctttccatt ctttgccggg tatatccggg aactgaacaa tggctgttcc
     3781 tttcaggtct atggctgaca gttgtgatgt atatacctgt gcttctgccc acaatgaaga
     3841 taggtcggct aacttcacta tgatgcttcc ttccgataca tactgtcctt ccatcaccgg
     3901 cagttcagta atatatcctg actcgttgct gtaaaaggaa gttagcggag aagctttttt
     3961 tgtttttgtc aattcattca cctgtgcttc actcataccc cataacagca attttgtttt
     4021 ggcactttga accagcctgt taaaatctat gatggaatta tcaagcgatt gttgtttttc
     4081 aactgctaca atgtattcct gctttgcatt attcaattcc tcactgtaca agtcatacag
     4141 gtgtgcacct tttgaaacat aatcacccat gtttttaaaa tacaacctgt ctatgcgccc
     4201 catgatacgg gcattgatgc tgtttacttt tgtttcgtct gtattcagtg ttgctgtaag
     4261 aatcatttta tccccaatca tcccattacg gatcgtgtct gtttgaatat tacccaagcg
     4321 gatttgttca tcattgagca atatttcatc tgcagcggta ttcgtttttt tcactacaat
     4381 cagatccatg tggcagatgg ggcatgttcc tggtttatcc tgcataatct gcgggtgcat
     4441 actacaggtg taatattcat ccttattgac tgttgttgtt ttcttgcttt tacatgaagc
     4501 caagacaaat aaaactgaag cgataattat caaatatttt ttcatagtca tttaatttgt
     4561 tttaatgaaa ctttcactgt ctaccaggta ttgtgcattg gcagcaacgg cttctgtttt
     4621 ttccagaccg cttaacacct gcacc
//
LOCUS       DAORTI010000203         4586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23641_length_4586_cov_5.859854, whole genome shotgun sequence.
ACCESSION   DAORTI010000203 DAORTI010000000
VERSION     DAORTI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_4586_cov_5.859854"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PLO70_15895"
     CDS             complement(<1..1545)
                     /locus_tag="PLO70_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76003.1"
                     /translation="MKILFKYLKPYKWLVALVLLLAAINIGFSLVDPIIFGKLVNLAN
                     HYVNNKNEYDPERFFWSFKENPHYGVAALLIASISVAMISRIAKAFQDYFLNLITQKF
                     GATVFTQGLQHAMKLPYQEFEDARSGETLSVLQKVRIDTEKFVASFINILFPVIIGIL
                     FVAVYATTIHPSLPFIYFGGIFLLTIISNLLSKKVKTIQKKIVGQTTALAGATTESLR
                     NIELVKSLGLTQQEVSRLNKNTFRILGLELTKVKRIRSISFIQGTFVNTLRQVILFML
                     LWLIFKEKMDAGQLVTMQIFSFFVFGPLQEIGNIILTYREAQASLENFGNLMKKAPEP
                     QAPNPKHLGQIETLEFDHVAFKHHTASHKAINDISFKVRTGETIAFVGPSGSGKTTLM
                     KLLVGLYRPQEGKILYNGMDENNIDFEDLRNQIGFVTQDTQLFSGSIKENLLFVNPAA
                     TDDELQSVLEKAACQNLLTRAENGLDTMIGEGGLKLSGGEKQRLSIARALLRMPKLLL
                     FDEATSA"
     gene            complement(1690..2454)
                     /locus_tag="PLO70_15900"
     CDS             complement(1690..2454)
                     /locus_tag="PLO70_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HQZ76004.1"
                     /translation="MKSSFQYLNDYFDKIVVLTLPRLTDRIAYINKTLEGLNFEFFFG
                     TDKEKTSIEELKEQGLYSTALYREFYKKPTEISTGMLCCALGHVSIYEYIIENGFQRT
                     LVLEDDVEPVYKHLPKFPVLTKELPEDWELLYLGYEKNETLGIREKLNRFMLTKLPHH
                     TQLKISREIFRNYYPVPFSPHIAKAGFHDCCHAYALTLEGAKKLLKHSKPVGFHSDNL
                     LSYLNCTGQLNGYISRLKLFNQLSAFVNKFDSLTAS"
     gene            complement(2641..4188)
                     /locus_tag="PLO70_15905"
     CDS             complement(2641..4188)
                     /locus_tag="PLO70_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQZ76005.1"
                     /translation="MLVKTFGSAVYGVEAISITIEVNWLTTGKDPMIVGLPDNAVKES
                     LQRIESTFKTNNYNVPRTKIVVNLAPADIKKSGTAFDLPIALGILGASEQIENSESLS
                     NYVIMGELSLDGEIRPIKGALPIAIQARKEGFKGLIVPKVNAKEAGMVNNLQVYGVTH
                     IKEVIDFFEKGEKGLEQIIINTREEFFNSQYDFEIDFADVKGQENIKRALEIAAAGGH
                     NAILIGPPGAGKTMLAKRLPTILPPLSLQEALETTKIHSVAGKLPENSTLVSKRPFRS
                     PHHTISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPMEERRVTIS
                     RAKVAVDFPASFMLMASMNPCPCGFYNHPERECTCPPGTVQKYLNKISGPLLDRIDLH
                     VEVTPVAFSELSSIKPQEYSSVIRDRVINAREKQAERYKNHPGIYCNAQMSSKMLKEI
                     CVISQVGANLLKAAMEKLNLSARAYDRILKVSRTIADLSQSEEIKPEHLAEAIQYRSL
                     DREGWAG"
     gene            complement(4259..>4586)
                     /locus_tag="PLO70_15910"
     CDS             complement(4259..>4586)
                     /locus_tag="PLO70_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76006.1"
                     /translation="EKVAGKNLETFFRQWLHTPVNPKLEIAWKYSPAEKKVIITVDQL
                     QQSVPFVFPLEIDLIADNKKTETRTLQITKQSETFHIPVSNNLKSIFPDPNTSLLFEA
                     RVIKMN"
BASE COUNT         1256 a          931 c          939 g         1460 t
ORIGIN      
        1 tgcagaagtg gcttcatcaa acaataataa tttgggcata cgtaataatg cccttgcaat
       61 agacaaccgt tgtttttcac cacccgataa tttaaggcct ccttccccga tcatggtatc
      121 aagcccgttt tcagccctgg tcagcaggtt ctggcaggca gctttttcca gtacactttg
      181 taattcatca tcagtggcag ccggatttac aaatagcagg ttctccttta tgcttcctga
      241 aaataattgt gtatcctggg tgacgaaacc aatctggtta cggaggtctt caaaatcaat
      301 attattttca tccataccgt tatataatat ctttccttcc tgcgggcgat ataaacccac
      361 taataatttc attaaggtcg ttttaccaga accagaagga ccgacaaaag caatggtttc
      421 ccccgttctg actttaaaac ttatatcatt gatagcctta tgcgaagcag tatgatgttt
      481 aaaagccaca tggtcaaatt ccagcgtttc aatctgtccg agatgtttag ggtttggtgc
      541 ctgtggttcg ggtgcctttt tcatcaggtt accaaaattc tccagtgagg cctgtgcttc
      601 ccggtaggta agaatgatat taccgatttc ctgcagggga ccaaatacaa aaaaggaaaa
      661 tatttgcatg gtgactaatt gccctgcatc cattttttct ttgaagatga gccagagcag
      721 cataaacaag attacctgcc tcagcgtatt tacaaaagtt ccctgtataa aactgatact
      781 gcggatccgt tttactttcg tcaattctaa tcccagtatt ctaaaagtat ttttatttag
      841 ccttgatact tcctgttgtg taagtccgag gctttttacc aattctatat tgcgcagtga
      901 ttccgtggtt gccccggcca gcgcagtggt ctgccctaca atcttcttct ggatggtctt
      961 aactttttta ctgagtaagt tggaaataat cgtaaggaga aatatccctc caaaatagat
     1021 aaaaggtaaa cttggatgaa tagtcgttgc gtaaacggct acaaaaagga tcccgattat
     1081 aaccgggaaa agtatattaa taaaggaagc cacaaatttt tctgtatcga tccttacttt
     1141 ttgtaaaaca ctcaaggtct caccgcttct ggcatcttca aattcctggt agggaagttt
     1201 catggcatgc tgcagcccct gtgtaaaaac agtagcgcca aatttttggg tgataagatt
     1261 caaaaaataa tcctggaaag ctttggcaat ccggctgatc atagcaacgg agatggatgc
     1321 tataagtaat gccgccactc cgtaatgcgg attttctttg aaagaccaga aaaaccgctc
     1381 cgggtcgtat tcatttttat tattgacgta gtgattagcc aggttgacca gctttccaaa
     1441 aatgatcggg tcaaccagtg aaaagccgat attgatagct gccagcaaaa gaaccaatgc
     1501 caccagccat ttataaggtt tcaggtattt aaataagatc ttcatacaaa cagttaatga
     1561 gtaaagggac agttatagca aaaatcatgg caaaagccaa actggtaata atgacattgt
     1621 tttgaaataa ttgcttgccg aatatacgaa ttggagggtt ttggctatgt cagcctgcca
     1681 tctttattac taactggcag taagagaatc aaatttgttt acaaaggcag acaactggtt
     1741 gaataatttc aggcgggaga tgtaaccgtt tagctgcccg gtgcagttca aatatgacaa
     1801 cagattatct gagtgaaaac cgacaggttt actgtgtttt agtagttttt tggcaccttc
     1861 aagggtaagg gcatatgcat ggcagcaatc atgaaaaccg gctttggcaa tatgtggtga
     1921 aaaaggaacc gggtagtagt tcctgaaaat ctcccggctt atttttaatt gtgtatggtg
     1981 aggaagtttt gtcagcataa aacggttcag cttttccctg ataccaagag tttcattctt
     2041 ttcatagccg aggtatagca gttcccagtc ttccgggagt tcttttgtga gaacagggaa
     2101 ttttggtaag tgtttatata caggttccac atcgtcttca agtaccaggg ttctttgaaa
     2161 tccattttca attatatatt cataaatgct gacatgaccc agcgcacagc aaagcattcc
     2221 ggtggatatc tctgtgggtt ttttatagaa ttccctgtat aatgcagtgc tgtaaagccc
     2281 ctgttcttta agttcttcaa tcgatgtttt ttctttgtct gttccgaaga aaaattcaaa
     2341 gtttaatccc tctaatgttt tattaatata agcaatacga tctgtcaaac gggggagtgt
     2401 tagcactacg atcttatcaa aatagtcatt caggtattgg aacgatgatt tcattaaagc
     2461 aataataagc aaataaactc cttgcctgta tagaaaatat aattttaaat tatctttttt
     2521 tcgtcaggcc aaacttattt ttaaatggat atttttactg gtgaactgca agcttattaa
     2581 ggaagtatta attgttggtt aaagaaattg tatttatagt tggtgttttt tatccaatca
     2641 tcagccagcc cacccttccc tgtcgaggct tctgtactga atggcttctg ccaggtgttc
     2701 cggttttatt tcttcgcttt gagacaagtc agcaatggtc ctggaaactt tcagaatcct
     2761 gtcataagcc cttgctgata agttaagttt ttccatagct gcctttaata aattggcccc
     2821 cacctggctg atcacacaaa tttctttcag cattttgctg ctcatctgtg cattacagta
     2881 gataccggga tggttcttgt atcgttctgc ctgtttttcc cttgcattga ttacccggtc
     2941 cctgataacg gacgaatatt cctgtggttt tatggaagaa agttcgctga atgcaacggg
     3001 tgttacttcc acatgcagat cgatacgatc taataatgga cctgaaattt tattcaggta
     3061 cttctgtact gtacccggcg ggcaagtgca ttctctctcc ggatgattat aaaaaccgca
     3121 ggggcagggg ttcatggatg ccatcagcat aaatgaagcg gggaagtcaa cagcaacttt
     3181 tgcccgtgag atagttactc ttctttcttc catcggctgg cgcattactt ccagcactgt
     3241 tcttttaaac tccggtaatt catccagaaa caaaacacca ttatgtgcca gtgatatttc
     3301 gcccggttgt ggtatgccac caccacctac caatgccaca tccgaaatag tatggtgtgg
     3361 cgaacggaag ggtcttttcg aaaccaatgt ggagttttcg ggtaacttac ccgctacgga
     3421 atgtatcttg gttgtttcta atgcttcctg taaactaagt ggcggtagta tagtgggcaa
     3481 tcgtttggcc agcatggttt ttccggcacc gggcggacca attaagatcg cattatggcc
     3541 accggcggct gcaatttcta atgctctttt aatgttttcc tgtcccttca catctgcaaa
     3601 gtctatttca aaatcgtatt gtgaattaaa aaactcctct cttgtattga tgatgatttg
     3661 ttcaagtcct ttttctcctt tctcaaaaaa atcaatcact tctttgatat gtgtcacacc
     3721 atacacctgc aggttattga ccatgccggc ttcttttgca tttactttag ggactatcaa
     3781 tcctttaaac ccttctttgc gtgcctgaat ggcaatgggt aaagctcctt tgatagggcg
     3841 aatctctcca tccagtgaaa gttcacccat gataacataa ttggaaagtg attcgctgtt
     3901 ttctatttgt tcagaagcgc cgaggatacc caatgcaata ggaagatcga atgcagtgcc
     3961 acttttttta atatcggcag gagccaggtt cacaacaatt ttggtgcggg gaacattata
     4021 gttattggtt ttgaaagtgc tttcaatccg ctgtaaactt tccttaacgg cattatccgg
     4081 aagccctacg atcatcggat ctttgcccgt agtcagccag ttaacttcga tggtgatgga
     4141 gatcgcttca accccatata ccgcactgcc aaatgtcttt actagcatgg ggggaagata
     4201 ataaaaaata ctaaccggtt tattaccgga aagtggtata taaatgccga tttctctttt
     4261 aattcatttt gatcaccctt gcttcaaaca ggagtgaagt gtttggatca ggaaaaattg
     4321 atttcaggtt attgctcacg ggaatatgga aggtttcgga ttgtttggtt atctgcagtg
     4381 ttcttgtttc tgttttttta ttatcagcga taagatctat ttcaagcggg aatacaaagg
     4441 ggacagattg ctgtaactgg tcaacagtga tgatcacttt tttttctgcg ggactatatt
     4501 tccatgcaat ttccagtttt ggattaaccg gggtatgcag ccattgccgg aaaaatgttt
     4561 caaggttctt accagctact ttctcc
//
LOCUS       DAORTI010000204         4570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23760_length_4570_cov_7.647619, whole genome shotgun sequence.
ACCESSION   DAORTI010000204 DAORTI010000000
VERSION     DAORTI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23760_length_4570_cov_7.647619"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLO70_15915"
     CDS             <1..234
                     /locus_tag="PLO70_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76007.1"
                     /translation="DGEFKGVCARGAFELDFAWKNKLVTQVSILSKAGEVCRIEYKPG
                     LKISSNGKNIKYKKLPNGLVEFQTIKGSSYQVK"
     gene            270..2501
                     /locus_tag="PLO70_15920"
     CDS             270..2501
                     /locus_tag="PLO70_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQZ76008.1"
                     /translation="MTYKFLSGLIFFIAFFSSGNVIAQNKKLTLEDIYTNGLYRSKGI
                     GQLRWMKDNKSYSTLEFNATAKGNDIVRYDVASGTRSVLVNANQLLPAGAGKPLSIDD
                     YTWSDDNNKLLIFTNTRKVWRYNTRGDYWVLNITNGKLKKLGKGLEDATLMFAKFSPD
                     GGRVAYVSMLNIYVEDIATGKITQLTKDGGDNIINGTFDWVYEEELDCRDGFRWSPDG
                     KNIAYWQSDTKGVGTFFMINNVDSNYSVPIPLPYPKVGTPNSAVKVGVIPATGGPTKW
                     FKVPGDPRNNYLARMDYIPGSDEVMIQQLNRLQNTNTVWVGNTKTMALKNILTDKDEA
                     FLDIHDNIAWLDNTTSFTWTSEKDGWMHLYKVSRDGKQMQLITKGNFDVVNINCIDPA
                     GGYVYYIASPENFTQRYLYRSRLDGNGGAERVSPANMAGQHSYQISADATYAIHSFEN
                     ATTPKRISLINLNTHAEIKLMEDNAALKNKINELGLRNKEFFKVDIGQVVLDAWMIKP
                     KDFDPQKKYPVIFHVYGEPAGSTVQDNWGTGDNLWHQYLANELGTVIISIDNRGTRVP
                     RGRDFRKCIYRQIGLLAADDQAAAAKKIVAMYPFIDAAHTGIWGWSGGGQMSLHCIFR
                     HGDVFKSAIAIAFVAHQALYDNIYQERYLGLPKDNVEGYREGSPITHAKKLQGNLMIM
                     HGTADDNVHYQNFEMLVNELIKHNKLFSMMSYPMRDHGINQGVNTTLHVRRTMEQFWK
                     KNL"
     gene            complement(2881..3342)
                     /gene="tnpA"
                     /locus_tag="PLO70_15925"
     CDS             complement(2881..3342)
                     /gene="tnpA"
                     /locus_tag="PLO70_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQZ76009.1"
                     /translation="MPNTYTQIHIHCIFAVKYRQALITKDWKERLHQYITGIVQNHTH
                     KMIAINSMPDHLHMLFGMRPTQSLSDLMRIVKGDSSEWINKMRLTPIPFRWQEGYGAF
                     SYEKQKISVVAAYIANQELHHQKRTFLEEYREFLANFEIDYDEQYVFKEPE"
     gene            3740..>4570
                     /locus_tag="PLO70_15930"
     CDS             3740..>4570
                     /locus_tag="PLO70_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76010.1"
                     /translation="MSTYPSIFNDVIGPVMRGPSSSHCAASLRIARLCRDLMEENIKN
                     ILIEFDPNGSLATTHKSQGSDMGLFGGFLGWEAFDERLPESDKHIVTAGINVTIHITD
                     IGNSHPNLYKITLSNNKETRKLTAISTGGGMIEVTAIDDVPVSMAGDYFETLIYCEKP
                     DAIVPFLQASVAYDEIALHQGAVSFIAIKSQAFLEAALYKELQQMEGVVCIKQLHPVL
                     PVMARKNLQVPFITCQEMLEYNKNKNKALWELAVDYEMARGNIAANEVLEKMRQVIHI
                     M"
BASE COUNT         1421 a          920 c         1022 g         1207 t
ORIGIN      
        1 gatggtgaat tcaaaggtgt ttgtgcaaga ggagcatttg agctggattt cgcctggaaa
       61 aataaattgg tcacacaagt aagtatttta tcaaaagccg gggaagtatg cagaattgaa
      121 tacaaaccag gtttaaagat cagcagcaac gggaaaaata ttaagtacaa gaaattaccc
      181 aatggattgg ttgagtttca aaccataaaa ggaagtagct accaggttaa atgatagtaa
      241 ttacttttta aaaaattaaa aacctaaaca tgacgtataa atttttatcg ggactaattt
      301 ttttcatcgc atttttttct tccggtaatg ttattgcaca aaataagaaa ctcacattgg
      361 aagatatcta taccaatggc ctgtatcgtt ccaaaggtat cgggcagctg cgttggatga
      421 aggataacaa aagctattcc acgctggagt ttaatgccac cgccaaagga aatgatattg
      481 ttcgttatga tgtggcaagc ggaacaagat ctgttttagt gaatgcgaat caattgctgc
      541 cggccggcgc aggcaaaccc ttatccattg acgattacac ctggtcagat gataacaaca
      601 agcttttaat atttacaaac acccgcaaag tttggcgcta caacacaaga ggtgattatt
      661 gggtattaaa tattaccaat ggaaaattaa aaaaactggg taagggcctg gaagatgcca
      721 cactgatgtt cgccaaattc tcaccggatg gcggaagggt ggcttatgta agcatgctga
      781 atatctatgt agaagatatt gcaacaggaa aaattacaca gctgacaaaa gatggcggag
      841 acaatatcat caacggcaca tttgactggg tgtatgaaga agaactggat tgccgggatg
      901 gttttcgctg gagccccgac ggaaagaata ttgcttactg gcaatcggac acaaaaggtg
      961 tcggtacttt tttcatgatc aataatgttg attctaatta ttcagtacct attccattgc
     1021 cttatccaaa agtgggcact cctaattcag ccgtaaaagt gggtgtgata ccggcaacgg
     1081 gcggaccaac aaaatggttt aaagtacccg gtgatccccg caacaattat ttagcaagaa
     1141 tggattacat ccccggctcg gatgaagtga tgatacagca gctgaaccgc ttgcagaata
     1201 cgaacacagt gtgggttggc aatacaaaaa ccatggcact gaaaaatatt ttgaccgata
     1261 aagacgaagc gttcctggat atacatgata atattgcctg gctggataac accacgtcat
     1321 ttacctggac cagtgaaaaa gatggctgga tgcacttata taaagtttcc cgtgacggaa
     1381 aacaaatgca gctgatcacc aaaggaaatt ttgatgtggt gaacatcaac tgtattgatc
     1441 ctgccggtgg ttatgtttat tatatcgcat cgccggaaaa ttttacacag cgttatctct
     1501 atcgcagtag gttagatgga aacggcgggg cagaaagagt atcgcctgca aatatggcgg
     1561 gccagcacag ctatcaaata tctgccgatg caacatatgc gatacacagt tttgaaaacg
     1621 ccaccacacc caaacgaatt tcacttatca atcttaacac ccatgccgaa ataaaattga
     1681 tggaagacaa tgccgcatta aaaaataaaa tcaatgaatt gggtttgcgg aacaaagaat
     1741 tttttaaagt agatattggt caagtagtgc tggatgcatg gatgataaaa ccaaaggatt
     1801 ttgatccgca gaaaaaatac ccggtcatct ttcatgtgta tggagagccg gcgggttcca
     1861 ctgtgcagga caactggggc accggcgata atctctggca tcaatacctc gccaatgaat
     1921 taggtacggt tataataagt attgataacc gcggcacccg ggtaccaaga ggccgtgatt
     1981 tcagaaaatg tatttaccgg cagattggtt tgctggctgc agacgaccag gcagctgctg
     2041 ccaaaaaaat agttgccatg tatcctttta ttgatgctgc acatacaggc atctggggct
     2101 ggagtggtgg cgggcaaatg tcattgcatt gcatcttccg tcatggggat gtatttaaat
     2161 ctgctattgc cattgctttt gtagctcacc aggcattgta tgataatatt taccaggaaa
     2221 gatatttggg cctgccaaaa gacaatgtgg agggatatag ggaaggttca cccatcacac
     2281 atgccaaaaa actacagggc aacctgatga tcatgcacgg cacagcagat gataatgtgc
     2341 attaccagaa ttttgaaatg ctggtgaatg aattaataaa acacaacaag ctgttcagta
     2401 tgatgtccta tcccatgcgg gaccatggca ttaaccaggg cgtaaacacc acactgcatg
     2461 taagaagaac aatggaacag ttctggaaaa aaaacctgta ggccttatag tcactcatgt
     2521 aaaaaagttt tgaaacacca agacaggggg acaccaggtc gccggggata taatttacat
     2581 tttaagaacc ctgtttttta aatctccgtg cctctgtgcc tttctgcctt cgtgtttcaa
     2641 agcaggcatc aaaacacaat gaatttcttc gtcccatagg gacgaaatgt cggtaacata
     2701 aaataccgac ttgttacttt gccctgtagg gcaaaacaaa agctcacccg gttaaatgat
     2761 gtacgcagtt aaaagtggtt cactaattac ttacaagagt tcttcgtccc gtagggactt
     2821 aatgccggta acaaaagtag tagagggata tctttgccct gtaggggcaa aacaaaacaa
     2881 tcactccggc tctttaaaca catactgctc atcgtaatca atttcaaaat tcgcaaggaa
     2941 ctcccggtac tcctccaaaa atgtcctttt ctgatgatgc aactcctggt ttgcgatata
     3001 ggcagccacc acactgatct tttgcttttc ataactaaaa gccccatacc cttcctgcca
     3061 gcgaaaagga atgggggtca acctcatttt attgatccac tccgaagagt ccccttttac
     3121 tattctcatc aggtcggaca aggattgggt tggccgcatt ccaaaaagca tatgcagatg
     3181 gtcaggcata gagttaatag cgatcatttt atgcgtatga ttctgaacga tgccggtgat
     3241 gtattgatgc agtcgttctt tccaatcctt tgttatcaat gcctggcggt acttgacggc
     3301 aaaaatgcag tggatatgta tttgtgtgta ggtattgggc actgtgaatg attttgtgtt
     3361 gcccctacgg ggcaaactgt tcctgtttta tttttctgtt accaacctat ggtccctacg
     3421 ggaccccatg caaccatcac taagctataa aaataattac agagtgtaag acacaggtca
     3481 tactggaagc aaaccgttcc tggtggttat tgcctcattt caacacgata aacatacagg
     3541 actaatacaa tcatgatcat taaactatag aaataactac agaatataaa actggtaaat
     3601 catgaaggaa gttaacggtc ccgggtatca gatgtttcat taatttcagt tggattaatt
     3661 agtttttttt agccaaccgg acatgggcga tacactggta aaaataattt gtatcttaaa
     3721 taattgtttc ttacaaacca tgagcactta ccccagcata ttcaatgatg tgatcggccc
     3781 ggtgatgcgg gggccatcca gttcgcattg cgccgcatcg ctgcgcattg cccgattgtg
     3841 cagagatttg atggaggaga atataaaaaa tattctcatt gaatttgacc ccaacgggtc
     3901 actggcaacc acgcataaaa gccagggctc ggatatgggt ttgttcggcg gcttcctggg
     3961 gtgggaagcc tttgatgaac gactgcccga atcggataag catattgtaa cagcaggaat
     4021 aaatgttacc atccatatta ccgacatcgg caacagtcat cccaacctgt ataaaataac
     4081 tttatcaaac aataaagaaa caagaaaact cacggccatc tccaccggcg ggggcatgat
     4141 agaagtgacg gccattgatg atgttcctgt ttccatggcc ggagattatt ttgaaacact
     4201 catttattgt gagaagcctg acgctattgt accattcctg caagcatcgg ttgcctatga
     4261 tgagatagcg ttgcaccagg gtgctgttag ttttatagcc atcaaatcgc aggctttttt
     4321 ggaggcggca ctatataaag agctgcagca aatggaagga gtggtatgta tcaaacagct
     4381 tcaccctgta ttaccggtaa tggcccggaa aaacctgcag gtgcctttta ttacctgcca
     4441 ggagatgctg gagtataata aaaacaagaa taaggctctt tgggagctgg cggttgatta
     4501 tgaaatggcc cgtggaaata ttgctgccaa tgaagtactg gaaaaaatgc gccaggtcat
     4561 tcatatcatg
//
LOCUS       DAORTI010000205         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23868_length_4557_cov_5.282097, whole genome shotgun sequence.
ACCESSION   DAORTI010000205 DAORTI010000000
VERSION     DAORTI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23868_length_4557_cov_5.282097"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLO70_15935"
     CDS             <1..994
                     /locus_tag="PLO70_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQZ76011.1"
                     /translation="QSLGIPIYDRHRAGGDTAATVKVFQLLLQNDKEQHIQKSLQRNS
                     KESVLPPNVPKEHFDQLPYTPGVYYFHNEKGKVVYVGKANNIRYRVNSHFSNNSQSRQ
                     KQNFLQHVHAISYQRCGTELMACILESTEIKKRWPVFNSSQKRWEDVYGIYLYEDQNG
                     YLRLVIDKNRKRLNPVYTFHYLVDGHAIIRKLIKEFKLCPKLCFLQKDAGKCEGIQEG
                     SCAGACEQLESPASYNSRIEKACASLRYQPSFAIVDKGLNGEDQSCILVLEGNFYGMG
                     YIPPDVPISDIELVKDHLTTYRENNFIRNLVNGYAARFPDKVVMLETISSQSFH"
     gene            complement(979..1416)
                     /locus_tag="PLO70_15940"
     CDS             complement(979..1416)
                     /locus_tag="PLO70_15940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQZ76012.1"
                     /translation="MESFSIPVQVRWSDMDPNFHLRHSVYYDWGAMSRVIFLEKYGLT
                     AELMQKLQFGPIIFREECVFRREIRMGEPVMITIELVHSKRDYSRWTIRHTIIKDSDT
                     VAAILTIDGAWLDTEKRKLTLPPQEAVDVFSQMPLSENFQWKD"
     gene            1510..2232
                     /locus_tag="PLO70_15945"
     CDS             1510..2232
                     /locus_tag="PLO70_15945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQZ76013.1"
                     /translation="MKKLILSVFVLITTISLCAQPGQTKPATKPAVPKPALPVLKTMN
                     DSASYAVGVSVANFYKQQGVKKLNTVLVSRAINDILGNKKPLIDDAAANTVMNNYMTM
                     LQAEKSKSVIEAGEKFLAQNKLRPEVKTTASGLQYEVLTEGSGEKPTAADSVTCHYRG
                     TFLDGKGFDNSYDRGQPITFALGGVIPGWTEGLQLMSVGSKYKFYIPYKLGYGAFDYM
                     TIPGGSMLTFEVELLEVRKKVQ"
     gene            complement(2369..2563)
                     /locus_tag="PLO70_15950"
     CDS             complement(2369..2563)
                     /locus_tag="PLO70_15950"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQZ76014.1"
                     /translation="MREASLIDANDLAAMAITTVGYDETIPLYKAGRIFTILLVAGCI
                     TYVFVITCIIQFSMGEKMSF"
     gene            complement(2758..4413)
                     /locus_tag="PLO70_15955"
     CDS             complement(2758..4413)
                     /locus_tag="PLO70_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ76015.1"
                     /translation="MKKILLTMGLFSLVLLGRAQTVLNEIYTTPGGTNSEFFELYNSS
                     TGIGGQNVDCFTIVTYYDNGGTDRGWYVMDLPDLTIGPKNFFVAAAASPFTTQNNAAP
                     GVVPNFNWNDPNFRNGSTGGSLTKWQVNGTGYTDVTASIPANLNNFMAGGGGEDYIVL
                     VFVNGVFNNGFIGGSGSLLSGSTAAPLPGNLPVVVNKAFHPCSDFTINFNSLGAMESV
                     GPQPGSDNGYARTSDGKCGAWAKTSNSVHHTPGVSNGSASGLTGSLTTAQLLQCNTAP
                     GVSTVTYNITAVSGDATEADDFPVEVQLYYDYGTPGQLDGADVFKFSQFDALVSDPQK
                     TFTINQTENVILVYKTKRGCFDKVFAIANGCLPLPVNLKSFTATRNRANVMLKWETMT
                     EQSSNGFAVERNIGNAGWEQITFVPSQAPNGNSSDLLTYQYIDPNNAKGITQYRIKQV
                     DIDNKSRLSEIRAVRGEGQLGKTIVYPNPTNDGRVTIVFEETNIVRDVTVMDMSGRII
                     RQLRSISNNNIQIDNLQPGMYSLRIVAAETGEQSVQKIVVNKR"
BASE COUNT         1404 a          960 c          898 g         1295 t
ORIGIN      
        1 ccagtctttg gggataccaa tctatgaccg gcatcgtgcc ggaggcgata ctgcggcaac
       61 cgtaaaagtc tttcagttat tattgcaaaa tgacaaagaa cagcatattc aaaaaagcct
      121 gcagcgtaat tcaaaagaat ccgtgttgcc gcctaatgta ccaaaggaac attttgatca
      181 gctgccttat acacccggtg tttattactt tcataatgag aaaggaaaag ttgtctatgt
      241 tggcaaagca aataatatcc gttaccgggt aaacagtcat ttcagcaata attcccaaag
      301 ccggcaaaaa caaaatttcc tgcaacatgt tcatgcaatc agctatcaac gctgcggcac
      361 tgaactgatg gcctgtatac ttgaatcaac agaaataaaa aaaaggtggc ctgtattcaa
      421 ttcttcacag aagagatggg aagatgtata tggcatctat ttgtatgaag accagaatgg
      481 ctacctgcgg ctggtcattg acaagaacag gaaacggtta aaccctgtat atacctttca
      541 ttatttagtg gatggtcatg ccatcatcag gaaactgatt aaagagttta agctttgtcc
      601 aaagctatgc ttccttcaaa aagacgccgg caaatgtgaa ggcatccagg aaggttcttg
      661 cgccggtgcc tgtgaacaat tggaatcacc tgcttcatat aatagccgta ttgaaaaagc
      721 ctgtgcatca cttcgctatc aaccttcctt tgccatcgtt gataagggat taaatggaga
      781 ggaccagtcc tgtattttag tgctggaagg aaatttttat ggtatgggtt atattcctcc
      841 ggatgtaccc ataagtgata ttgaattagt aaaagatcat ctcactactt acagggagaa
      901 taattttatc cgtaatcttg tgaatggata tgctgcccgt ttcccggata aagtggtaat
      961 gctggaaaca atctcctctc aatctttcca ttgaaaattt tcactaagag gcatctgcga
     1021 aaacacatca actgcctcct gtggtggtaa agtaagtttc cttttttctg tatcgagcca
     1081 ggcaccgtca atagtaagga ttgcagctac ggtgtccgaa tcttttataa tagtatgccg
     1141 gatggtccac ctggaatagt ctctttttga atgaaccagt tcgatcgtta tcattacagg
     1201 ttctcccatc cgtatttccc gtcggaaaac acattcttcc cggaatatga tgggaccgaa
     1261 ttgaagcttt tgcatcaact cagcagtaag accatatttc tccaaaaata tgacacggct
     1321 catggcaccc caatcgtaat ataccgaatg ccggaggtga aaattcggat ccatatcaga
     1381 ccaacgaacc tgtactggta ttgaaaaaga ctccattttt tactttattt aacagttaaa
     1441 attaaatgat tgaaggtatc ggatttccct tttcaacctg tatttttgac aacttaacag
     1501 atatataata tgaaaaaatt gatcctatcc gttttcgttc tgattacaac tatctccctg
     1561 tgtgcccaac ccggtcaaac gaagcctgca actaaacctg cagtgcccaa acctgcttta
     1621 ccggttttaa aaaccatgaa tgactcagca agttatgctg tgggtgtaag tgtggccaat
     1681 ttttataaac aacaaggcgt taaaaagctt aatacagtac tggtttcccg tgctatcaat
     1741 gatatcctcg gaaataaaaa gccactaatt gatgatgctg cagcaaatac agtaatgaat
     1801 aactatatga ctatgctgca ggcagaaaaa tcaaaatccg taatcgaagc tggtgaaaaa
     1861 tttttagccc aaaacaaact ccggccagaa gtaaaaacaa ctgccagtgg gctgcaatac
     1921 gaagtactta ctgaaggatc aggtgaaaaa ccaacagccg cagatagtgt aacctgccac
     1981 tacaggggaa ctttcctgga tggtaagggc tttgacaatt cgtatgatcg tggtcaacct
     2041 atcacttttg cattgggtgg tgttattccc ggatggacag aaggattgca actgatgagt
     2101 gtgggttcca agtataaatt ttacattccg tataaacttg gttacggagc atttgattat
     2161 atgaccatcc cgggtggctc catgctgaca tttgaagtgg aattacttga agtaagaaag
     2221 aaagtgcaat aattaatttc ccaatctgat ctttatcgtc ctactacata aaatctcacc
     2281 aggataacct ggtgagattt tttaattaat cctgtgcaca atcattgaca gaccaggcta
     2341 tcttatatta gcatgcctga tctaaaattc agaatgacat cttctctccc atactgaatt
     2401 ggatgataca ggttattaca aatacatacg taatacagcc ggctaccagg agaattgtga
     2461 atatccttcc ggccttgtat aatggtattg tttcatcata accaactgtg gtgatagcca
     2521 tggccgccag gtcgttcgca tcaattaagg aagcctctcg aataatatat actccaatat
     2581 acctgtgaca actataatag gaagaatgat aaagggctgt aataacttgc ggggaaactt
     2641 ttcaagcagg ttatttgtca taacagtata acaggagaag ttagaaaagt aggagcaaaa
     2701 aaaaggggga cagccaaaaa gccatccccg taggatttaa ttaaaaaccc tgagcattta
     2761 gcgtttgttc acaacgatct tctgcacaga ctgctcaccg gtttctgctg caacaattcg
     2821 taaactgtac ataccgggct gcaggttatc aatctgaata ttattattcg agatacttct
     2881 caattgcctg atgatcctgc cgctcatatc catcacggtt acatccctta cgatgttggt
     2941 ttcttcgaaa acgatcgtaa ccctgccatc attggtagga ttagggtaaa caatggtttt
     3001 acctaactgg ccttcgccac gaactgcacg gatttcactt aatcttgact tgttatctat
     3061 atccacctgc ttgatgcggt actgagtgat accctttgca ttattgggat caatatactg
     3121 gtacgtcaaa aggtcgctgc tgttaccatt aggtgcctgt gatggtacaa atgtaatttg
     3181 ctcccaacct gcattaccga tattacgttc tactgcaaaa ccatttgaac tctgctcggt
     3241 catagtttcc catttcagca ttacattagc acggttgcgg gtagcagtaa aggatttgag
     3301 attaacgggt agaggcaaac atccatttgc gatagcgaaa accttatcaa aacaacctct
     3361 ctttgtttta taaacgagaa taacattttc tgtttgatta attgtaaagg ttttctgtgg
     3421 gtcagacact aacgcatcaa attgtgaaaa cttaaataca tcagcaccgt ctaactgacc
     3481 gggtgtacca taatcatagt ataactgaac ttcaacaggg aagtcatcgg cttcagtagc
     3541 atcgccggaa acggcagtaa tattatacgt tacggtagaa accccggggg cagtgttaca
     3601 ttgcagtaac tgagctgttg tcaacgatcc ggtaagacca gaggctgaac cgtttgaaac
     3661 tcccggagta tggtgcacac tgttggatgt ttttgcccag gcaccacatt tgccatcact
     3721 tgtacgggca taaccattat cggaaccagg ttgtggccct acagattcca ttgcgcccaa
     3781 gctgttaaaa ttgatcgtaa aatcagaaca tggatgaaaa gctttattga caactactgg
     3841 caaatttccg ggtaatggtg cagcagtaga acctgataac aatgaaccac taccacctat
     3901 gaaaccatta ttaaaaacac cattcacaaa aaccaaaaca atataatcct caccccctcc
     3961 accagccatg aaattattca aatttgctgg tatgcttgct gttacatccg tgtatcctgt
     4021 cccattaacc tgccattttg ttaaagaccc tcctgtgctt ccatttctga aattcgggtc
     4081 attccagtta aaatttggca ctaccccggg agctgcatta ttttgtgttg taaaaggact
     4141 ggcagcagca gcaacaaaga aatttttggg accaattgtc aggtctggta agtccataac
     4201 ataccaacct cggtcagtac caccgttatc gtaatacgtt acaatcgtaa aacaatctac
     4261 attctgaccg ccgataccgg tactgctgtt atacaattca aaaaattctg aatttgttcc
     4321 acctggagtg gtgtaaatct cattaagtac tgtctgggct cttcccaaga gtacaaggct
     4381 gaataagccc atagtaagta aaatcttttt catgcttcag gttttaaatt aaaaaataat
     4441 tagttgcttt cacaacgata tatgacgaat cagtacatcc acaattgatt gtggtgattc
     4501 aatggaatgg accttgaagc attcaaatgg agataggatc tatacaagta gttcaga
//
LOCUS       DAORTI010000206         4545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_23960_length_4545_cov_7.355457, whole genome shotgun sequence.
ACCESSION   DAORTI010000206 DAORTI010000000
VERSION     DAORTI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23960_length_4545_cov_7.355457"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLO70_15960"
     CDS             complement(<1..524)
                     /locus_tag="PLO70_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HQZ76016.1"
                     /translation="MALKDIQSTGNKDTRSGFGDGIVEAARKNPNIVALTADLAGSLK
                     LNQFMKEFPERFIQCGIAEANMIGIAAGLTIGGKIPYTTTFANFSTGRVYDQIRQSVA
                     YSDKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVIVPCDYAQTKAATEAIA
                     DYDGPVYLRFGRPV"
     gene            complement(598..1653)
                     /gene="galE"
                     /locus_tag="PLO70_15965"
     CDS             complement(598..1653)
                     /gene="galE"
                     /locus_tag="PLO70_15965"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQZ76017.1"
                     /translation="MAKILVTGGCGYIGSHTIVDLIENGYDVISVDNNSRSNPNVLLG
                     VEQITGKKVKNYKVDLCNFDDTFAIFQENVDITGIIHFAAYKAVGESVENPLMYFENN
                     LVSLINLLKCVQEFHIPHFVFSSSCTVYGNPDEIPVTEKTQPKPAESPYGYTKQMSEQ
                     IINEFAKSSGTQNILLRYFNPAGAHPSAIIGEMPIGKPQNLVPAITQTAIGRIEKMYV
                     YGNDYPTRDGSCLRDFIHVCDLANAHTLALKYLEEGRSNGLCEVFNLGSGNGVTVLEA
                     IHAFEKESGIKLNYEIGARRAGDVVAIYANNDLAKKMLGWNPKFSLDEIMASAWKWEL
                     RLKADETVFTSQAGDLN"
     gene            complement(1705..2916)
                     /locus_tag="PLO70_15970"
     CDS             complement(1705..2916)
                     /locus_tag="PLO70_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQZ76018.1"
                     /translation="MTPFLITIIALALVFDYINGFHDAANSIATVVSTKVLTPFQAVL
                     WAAVFNSVAFFIFKDHGVADTVAKTVKPEHIMEGNMMVVIFSGLIAAIAWNLLTWWLG
                     IPSSSSHTLIGGFAGAAVAAGGFEAVNSSIIIKTASFIFLAPLIGMLMAFIMSLWFLS
                     SFKTGWTSKIFSVGVIVFVVIFLAVSLEFDKEVLAKKTDYENYISRVIFYSKNFKWIL
                     LCFILLVMASFSLYLSNLNAHKANQWFKRLQLISSAAFSIGHGGNDAQKVMGIITAAL
                     IAGGSITDFSQMPNWVPISCYLAIGLGTMSGGWKIVKTMGTKITKVTPFEGVAAETAG
                     ALTLFITEALKIPVSTTHTITGAIMGVGATKRLSAVRWGVTINLIWAWILTIPISALL
                     AAGVYQVARLF"
     assembly_gap    3178..3277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3278..3498)
                     /locus_tag="PLO70_15975"
     CDS             complement(<3278..3498)
                     /locus_tag="PLO70_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HQZ76019.1"
                     /translation="MAGLQSFLKIFMPKNRIFYELFEKVADNVSKMGSQLKDVVAEPD
                     FDKRAALITQIEDLEHANDELTHNLFTELG"
     gene            3674..>4545
                     /locus_tag="PLO70_15980"
     CDS             3674..>4545
                     /locus_tag="PLO70_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQZ76020.1"
                     /translation="MAKRIVSKVLLSTILVAFLSIKGNSQRYLSDIDSIFFIKDTVRP
                     VIKRFENLRITGYIQPQFQKAQSDGAPSYTGGNFSQYSSSRFMLRRARVKIDYIMPSK
                     EKYPQAMFSFQIDATERGVIVRDMFLKLFETKRNLFSMTAGLFARPFGYEVNLSSAFR
                     ETPERGRMSQILMPSERDIGIMVSHEPQESKQKRSRFKFDIGFFNGQGLSGTTDFDSH
                     KDLISRMFIRPNTSDKVEFSGGLSLLRGGWKNGTKYVYESGKAPNGDGIFVVDSSISN
                     IGKSSPRHYYGADA"
BASE COUNT         1339 a          926 c          895 g         1285 t
ORIGIN      
        1 cacacgggtc ttccaaaacg caggtacacc ggaccatcat aatcggctat agcttctgtg
       61 gcagcttttg tttgtgcata atcacaggga acgataactg tcatgcccgg caacattttc
      121 atcaatccaa tgtcttccag tatttggtgt gttgcaccat cttcacctaa tgtaagacct
      181 gcatgcgaag cacagatttt tacattttta tcgctataag caactgattg acggatctgg
      241 tcataaacac gaccagtgga gaagttggca aaagtggtgg tgtaaggaat tttgcctcca
      301 atagtcaaac cagccgcaat gcctatcata ttagcttctg caatgccgca ttggataaaa
      361 cgttccggaa attccttcat gaactggttt agcttgagcg atcctgccag gtcagcagta
      421 agggcgacaa tattcgggtt ttttcttgcc gcttccacaa taccatcacc aaaaccactt
      481 cgggtatctt tgtttcctgt tgattgtata tcctttaatg ccatgattaa aatttatgtg
      541 tcgcaaaagt aagggatagg ggtgaatggt ggatagtcaa tagtgaacag cgaaaaatca
      601 gttcaggtcc cctgcctggc tggtaaagac ggtttcatcc gctttcaaac gcagttccca
      661 tttccaggcg gaagccatga tctcatccaa cgaaaacttc gggttccaac caagcatttt
      721 tttagctaaa tcattatttg catatatggc aacaacatca ccggcccttc tggcgcctat
      781 ttcataattc agtttaatac cgctttcttt ttcaaatgca tgtatggctt ccagtactgt
      841 tacaccattg ccactaccca ggttaaaaac ctcacacaaa ccattgcttc ttccttcttc
      901 caggtatttc aaagctaaag tatgggcatt ggcaaggtcg caaacatgaa taaaatcccg
      961 caggcaggaa ccatccctgg ttggatagtc attgccgtaa acatacattt tttcaatacg
     1021 gccaatagca gtttgagtta tagcaggaac aagattttga ggtttaccaa tgggcatttc
     1081 accaataata gctgaagggt gtgccccagc cgggttgaag taacggagta aaatattttg
     1141 agtcccagaa cttttggcaa attcgtttat gatctgctca ctcatctgtt tggtatagcc
     1201 atagggagat tctgccggct tgggctgcgt tttctctgtt acaggtatct catcaggatt
     1261 gccatatact gtgcaggaag aagaaaaaac aaaatgggga atatgaaatt cctgaacgca
     1321 tttcagcagg ttgatcagag aaaccaggtt gttctcgaaa tacatcaaag gattttccac
     1381 ggattcacca acagctttgt atgcggcaaa atgaataata ccggtgatgt caacattttc
     1441 ctggaaaatg gcaaacgtat catcaaagtt gcacaggtct actttatagt tctttacttt
     1501 tttgccggtt atctgttcta cacccaacag tacgtttggg tttgaacggg aattgttatc
     1561 aacggaaata acatcatatc cattttctat caggtccaca attgtgtgtg aaccaatgta
     1621 gccgcagccg cctgttacta gtattttagc cataaaaaat cccgacaaaa gtcaggattt
     1681 attttataaa accggtgtat taaattaaaa taacctggca acctggtaca ctccagctgc
     1741 cagtaaggca ctgataggga ttgttaatat ccaggcccag ataagattga tcgttacgcc
     1801 ccatcttaca gcagacaatc tttttgttgc gccaactccc atgatagcac ctgtaatagt
     1861 gtgtgtagta ctcaccggaa ttttcaatgc ttcagtaata aacaatgtta acgcaccagc
     1921 agtttctgca gccaccccct caaaaggagt aaccttggta atctttgtgc ccattgtttt
     1981 tacaatcttc cagccaccac tcatcgttcc taaacctatt gccaaataac aggagatggg
     2041 aacccaattg ggcatctggc tgaaatcagt aatagacccg cctgctatca gggcagcggt
     2101 gataataccc attacttttt gggcatcatt acctccatga ccgatactga aggcggcaga
     2161 agaaatcaat tgtaatcttt tgaaccattg atttgcttta tgtgcattca ggttgctcag
     2221 gtaaagcgaa aaagaggcca ttacgagcaa aatgaagcat aataggatcc atttgaagtt
     2281 ctttgagtag aagattaccc ggctgatata gttttcataa tctgtctttt tagccagcac
     2341 ttctttatca aactcaagac taacagcgag aaaaatgacg acaaaaacta tcaccccaac
     2401 ggaaaatatt tttgatgtcc atcctgtttt aaaagaactt aagaaccata acgacatgat
     2461 aaatgccatg agcataccaa tgagtggtgc caggaaaata aagctggccg ttttaattat
     2521 gatcgatgag ttaactgctt caaatccccc ggcggcaacg gctgcccctg caaaccctcc
     2581 aattaatgta tgagacgatg aagaagggat acccagccac caggtaagca ggttccacgc
     2641 aatggctgca ataagtccgg aaaatataac gaccatcata ttgccttcca taatatgttc
     2701 tggtttaaca gtcttggcta ccgtatctgc cacgccatga tctttgaata taaagaaggc
     2761 cactgaatta aatacagcag cccagagtac tgcctggaag ggagtaagca ccttggttga
     2821 aaccacggta gcaatagagt tggccgcatc atgaaaacca ttgatataat caaataccag
     2881 tgcaagggct atgatggtga tcaggaaagg tgtcataaaa gttaattaga aaattgctca
     2941 atacagcaat actcaatatg tcataaacag gaggcgttat tgagcaattg agccattttc
     3001 aaattgagta aaaattgatt aggcatattt aataataata gactctatca cattactggc
     3061 atcttcgcat ttgtcagtaa cgatctccat tacctggtat atctctcttt tcttgatcac
     3121 ttctttggca tccggttcag tggcgaataa acgttcaatg ctcatatcaa aaatatcnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc agttcagtga acaggttatg
     3301 ggtaagttca tcgttagcat gttccaggtc ttcaatctgg gtaataagtg ctgccctttt
     3361 atcaaaatcg ggttcggcca ccacatcttt cagctgcgaa cccattttag aaacattatc
     3421 agctactttt tcaaacagtt cataaaagat cctgtttttg ggcataaaaa ttttcagaaa
     3481 tgattggagt cctgccatta gttttagttt tattcccgtt tacagcggga ttgtttgcga
     3541 aaataccggg tacagcactc agggtaacga agggacggag aattaatgat aaattaatat
     3601 agaaataaaa ttagggtaac aattcaataa caaagacttt atattgcaac aattttaagc
     3661 aatgcatatg tttttggcta agcgaatagt gtcaaaagtc ctcttatcca ccattttagt
     3721 tgcttttttg agtataaaag gtaattcaca acgatacctc tctgatatcg actcaatttt
     3781 ttttataaaa gacacagtaa ggccggttat caagcgattt gaaaacttaa ggatcacagg
     3841 atatatccag ccgcaatttc aaaaagctca atctgacgga gctccttcct ataccggagg
     3901 taatttttca caatattcca gtagccgctt catgctccgg cgtgcaaggg taaagattga
     3961 ttatataatg ccttccaaag aaaaatatcc ccaggccatg ttttcttttc agatcgatgc
     4021 aacagaaagg ggagtgattg tacgggatat gtttttaaaa ttatttgaaa caaagagaaa
     4081 tcttttttca atgacggctg gtttgtttgc ccgtcctttt ggttacgaag ttaacttatc
     4141 ctctgccttc agggaaacac cagaaagggg caggatgtcg caaatattaa tgccctcaga
     4201 aagggatatt ggaataatgg tttctcatga accgcaggaa agcaagcaaa agcggtcccg
     4261 tttcaaattt gatattgggt tttttaacgg gcagggtttg tcaggcacaa ctgattttga
     4321 tagtcacaaa gatcttatat caaggatgtt catcaggcca aatacatcag ataaggttga
     4381 attttccggt ggcctgtctc tcctgcgtgg tggctggaag aatgggacca aatatgtgta
     4441 tgaaagtgga aaggctccta atggagatgg aatttttgtc gtcgattcat ctatctctaa
     4501 tattggaaaa tcttctccca ggcattatta cggggcagat gccca
//
LOCUS       DAORTI010000207         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_24214_length_4510_cov_8.010101, whole genome shotgun sequence.
ACCESSION   DAORTI010000207 DAORTI010000000
VERSION     DAORTI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24214_length_4510_cov_8.010101"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1476)
                     /locus_tag="PLO70_15985"
     CDS             complement(238..1476)
                     /locus_tag="PLO70_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQZ76021.1"
                     /translation="MIQFEKFTLANGLRVIVHQDLSTPMAVMDIMYDVGARDEDPNRT
                     GFAHLFEHLMFGGSVNIPSYDEPLQMAGGENNAYTTNDLTNYYIQLPAENLETAFWLE
                     SDRMLSLAFGDKSLETQRKVVCEEFKEHYLTKPYGDVWHKMRELAYKKHPYRWMTIGK
                     ELSHIENAQLEDVKAFFFKHYRPVNAVLVVAGYVSVEKVKVLAEKWFGDIPSGERYKR
                     NLPQEPEQTEARKLEIKANVPLDALYKTWHIKNRLDRKYYITDLLTDILSGGGSSRLY
                     QSLVKEKQLFSNIECYHFGSTENGLLSIEGKLVKGVKIEDAEKAVEQELDKMKTELVP
                     EAELQKVKNKTESMIAFEDMSVMNRANSLAYYETLGDAAWMNYELQKYGTVSSEDILQ
                     ESRNIFRAENSNTIYYLSDN"
     gene            complement(1574..2770)
                     /locus_tag="PLO70_15990"
     CDS             complement(1574..2770)
                     /locus_tag="PLO70_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HQZ76022.1"
                     /translation="MKRAFSCFVLCFVWLAGTPLAAQEEFIEPPSQRLTSVSFHQLTG
                     GIVLLQARIDSFPDTLNFILDTGSSGISLDSSTVDYLGLKPTPTDRTIRGIAGIRNVS
                     FLYDRQLHFPGLTIDNLNFHINDYEILTAVYGERIDGIIGYSVFSRYIMKLDYDSMKL
                     TFFTKGTFRYPKGGYLLKPSISQLVTQPLRVRDAETVYSRFLYDMGAGLCLLLSREFV
                     DDSALIHKKRKRWVKEGEGLGGKIDMELTVIKEVRVGPYKFRNVPIFVFDDVYNVTSY
                     PYMGGLLGNDILRRFNVILNYAKGDIYITPNTHFSDVFDYSYSGVELYLIGGRIILGD
                     IAKGSPAEACGLKEGDEVIAVNKNFSQNLNQYKIALQVPNEKIKIIVRRDGVMRDFEF
                     KVRSIL"
     gene            2955..4079
                     /gene="mqnC"
                     /locus_tag="PLO70_15995"
     CDS             2955..4079
                     /gene="mqnC"
                     /locus_tag="PLO70_15995"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HQZ76023.1"
                     /translation="MQLNELYDKASAFGYLSVEEGVYLYHHAPLADLMFIADELRKKQ
                     VPHGKVTWQIDRNVNTTNVCIANCKFCNFYRIPGHAEAYITDMPTYRQKITETFKYGG
                     DQLLLQGGHHPELGLQFYVDTFRQIKEEFPTIKLHALGPPEVAHITKLEKSTHHEVLK
                     ALKEAGMDSLPGAGAEILIDRVRRLISKGKCGAQEWLDIMHEAHKLNITTSATMMFGH
                     VETIEERFEHLVKIREVQSRKPEHAKGFLAFIPWTFQDVDTLLAKIRGVQNLTTAEEY
                     IRMIALSRIMLPNVKNIQASWLTVGKETASICLQAGANDFGSIMIEENVVSAAGAPHR
                     FTYKTIQDAIREAGFQPQLRNQQYEWREIPETIEEQVIDY"
     gene            4324..>4510
                     /locus_tag="PLO70_16000"
     CDS             4324..>4510
                     /locus_tag="PLO70_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HQZ76024.1"
                     /translation="MKKSYSTFRKVAFAEGVSFLILLFIAMPLKYFGDMPKAVTIVGS
                     LHGILFVAFGVLSLDVKN"
BASE COUNT         1272 a         1053 c          930 g         1255 t
ORIGIN      
        1 attcctttct ttttaatatc gtttcggtaa tcacccttcg acaatctcag ggtgacattg
       61 tgatttcatt catcaccctt ttcttagttt cactcaggat aaactactca gggtgacaga
      121 aagagtgtca ggctgagctt gccgaagccg ggttagaatt tcgttagtct ccctttcctt
      181 cgttgcactc agtataaact actccgggcg acgctataat ttctctcctt agtcaattca
      241 attatcggac aaataataga tcgtattcga attttcagcc ctgaaaatat tccggctctc
      301 ctgcaaaata tcttcagaac ttactgttcc gtatttctgc aattcataat tcatccaggc
      361 tgcatcaccc agcgtttcat aatacgccag gctattggcc ctgttcatca cactcatatc
      421 ttcaaatgcg atcatgcttt ccgttttatt cttcaccttc tgcagttctg cttcaggcac
      481 cagttctgtt ttcattttat ccagttcctg ctccactgct ttttcggcat cttctatctt
      541 cactcctttt accagtttac cttctatgga caacaaacca ttttctgtgc tgccaaaatg
      601 ataacattct atattgctga acaattgctt ttctttcacc agtgactggt acaatcttga
      661 tgaacctcct ccgcttaaga tatctgtcag cagatcggtg atataatatt tcctgtcgag
      721 acggttttta atatgccatg ttttatacaa cgcatccagc ggtacattgg ctttaatttc
      781 cagtttcctt gcttctgtct gttcgggctc ctgcggcagg ttacgtttat atctttctcc
      841 cgaaggaata tcaccgaacc atttttctgc caatactttt actttttcta ctgacacata
      901 tcctgctaca accagtactg catttaccgg gcggtaatgt ttaaagaaaa aggcctttac
      961 atcttccagt tgtgcatttt caatatgcga taattcttta ccgatcgtca tccagcggta
     1021 aggatgcttt ttgtatgcaa gctcccgcat tttatgccac acatccccat acggtttggt
     1081 gagataatgt tctttaaatt cttcacatac cactttacgt tgtgtctcca ggcttttatc
     1141 tccgaatgcc agcgacaaca tacggtcact ctctaaccag aaagcggttt ccaggttttc
     1201 tgccggcaac tggatatagt aattagtaag atcgttggta gtataagcat tgttctcgcc
     1261 accggccatc tgcaatggtt catcataact gggaatattg acagaaccac caaacatcag
     1321 gtgttcaaac aaatgggcaa acccggtacg gttgggatct tcatcccttg cccccacatc
     1381 atacatgatg tccatcaccg ccatcggtgt actgagatcc tggtgaacaa tgacccgcaa
     1441 accattggcc agggtaaatt tttcaaactg tatcatactg gtaataacaa gtaaaggaat
     1501 agttagttgt aataggtaaa agtaccgaaa aatgtggctg cagatgctac cggtcatgcc
     1561 acgaaaggga aaatcagaga atgcttctca ccttgaattc aaaatccctc atcactccat
     1621 ccctgcggac gatgatcttt attttttcat tgggaacctg cagggcaatt ttatactgat
     1681 tcaggttttg tgaaaaattc ttatttacgg ctatcacttc gtccccttct ttcaacccgc
     1741 aagcttccgc cggagaacct ttggcaatat caccgagtat tatccgcccg ccaataagat
     1801 acaattccac tcccgagtag gaataatcga aaacatccga aaagtgagta ttgggggtaa
     1861 tataaatatc gcctttggca tagttgagta tcacattgaa gcgccgcaaa atatcattcc
     1921 cgagtaaacc tcccatatac ggataggagg taacattata cacatcatca aaaacgaaga
     1981 tgggcacatt cctgaattta tagggcccca cccttacctc ttttatcacc gtcagttcca
     2041 tatcaatctt ccctcccagt ccttctcctt cttttaccca gcgtttcctt tttttatgga
     2101 tgagagcact gtcatctaca aattctcttg acaataacag gcaaagcccg gcacccatat
     2161 catataagaa acgggaatag accgtttcag catctctgac ccgcagcggc tgggtcacca
     2221 actggctgat ggaaggtttc agcaggtaac cacctttggg atagcgaaaa gtgcctttgg
     2281 tgaaaaaagt aagcttcata ctgtcgtagt ccagcttcat gatataacgg ctgaagacag
     2341 aataaccaat gatgccatca atgcgttccc cgtaaacggc cgtcagtatt tcataatcat
     2401 tgatatgaaa attgaggtta tcgatcgtaa ggccgggaaa atgtaactgg cggtcataca
     2461 ggaaactcac attcctgata cctgcaatac cccgtatcgt tctgtcagta ggagtgggct
     2521 tcagcccgag ataatccact gttgaagaat caagggaaat accgctgctg ccggtatcca
     2581 gtatgaaatt gagtgtatcc ggaaaactgt caattcttgc ctgcaacagg acaatacccc
     2641 cggtaagctg gtgaaatgaa acggaagtaa ggcgctggga aggcggttca ataaactcct
     2701 cctgtgcagc cagcggcgta ccggccagcc acacaaaaca caggacaaag caagaaaatg
     2761 ctctcttcat acagtaagtc ataaaaagcg taaaatcatc cacggttctg aactttctct
     2821 ttgctatgac aggtatttta ttcaaaatga tgcaatacag gctgctattt attgtaatta
     2881 aatgtactct attaccattc cggcacaaaa ccgttcaacc aatttcttgt tattgaggtg
     2941 taactttgcc gcttatgcag ctaaacgaat tatacgacaa agcctctgct tttggctacc
     3001 tgtcagtgga agaaggagtt tatctctatc atcatgcccc cctggccgat ttgatgttca
     3061 tcgcagatga gctcagaaaa aaacaggtgc cgcatgggaa agtgacctgg cagatagacc
     3121 gcaatgtgaa caccaccaat gtatgtatcg ccaattgtaa attttgcaat ttttaccgca
     3181 tacccgggca tgccgaagcc tatatcacag atatgcccac ctaccggcaa aagataacgg
     3241 agacatttaa atatggcgga gaccagttgc tgttgcaggg tggccatcat cctgaactgg
     3301 gtttgcagtt ttatgtggat accttccgcc agataaaaga agaattcccg acgatcaaac
     3361 tgcatgcact cgggccgccc gaagtagccc atatcaccaa actggaaaaa agtacgcacc
     3421 atgaagtgct gaaagcattg aaagaagcgg gtatggattc attgcccggt gccggtgccg
     3481 aaatattaat tgacagggtg agacgattga tctcaaaagg aaaatgtggt gcacaggaat
     3541 ggctcgacat tatgcatgaa gcacataagc tgaacatcac cacttctgcc acgatgatgt
     3601 ttggccatgt ggagacgatc gaagaacggt ttgagcattt ggtaaagatc agggaagtgc
     3661 aaagccgtaa accggaacat gccaaagggt ttttagcttt tattccgtgg accttccagg
     3721 atgtagatac actgctggcg aaaataagag gggtacaaaa cttaacgact gcggaagaat
     3781 atatccgcat gatcgcctta agcaggatca tgttgccgaa tgtaaagaac atacaggcca
     3841 gctggctgac tgttggtaaa gagacagctt caatttgttt gcaggcgggg gccaatgatt
     3901 ttggttccat catgatcgaa gagaatgtgg tgagtgcggc cggtgccccg catcgcttca
     3961 cgtataaaac aatacaggat gccattcggg aagccggttt tcaaccgcag ctgcgtaacc
     4021 agcagtatga gtggagagaa attccggaaa cgatcgaaga gcaggtaatt gattattaac
     4081 ccggcttcgg catcccgata gctatcggga cagcctgaca ctctttctgt caccctgagt
     4141 ttatcctgag tgaaacgaag ggaagggtga ttaacgaaat ctttatcctg agtgcaacga
     4201 aggaaagggg gactaacgaa attctaaccc ggcttcggca agctcagcct gacaatcatc
     4261 ttgtcaccct gagtttatcg aagggtgata aacgaaaaac aacaaaaaaa cgtacagcat
     4321 ataatgaaga aatcatacag tacattcaga aaagtagctt ttgcagaagg ggtttccttt
     4381 ttgatcttat tattcattgc catgcccctg aaatattttg gcgatatgcc caaagccgta
     4441 accattgtgg gcagtttgca tgggatcctc tttgtagcat ttggtgtatt atccctcgat
     4501 gtaaaaaaca
//
LOCUS       DAORTI010000208         4501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_24275_length_4501_cov_5.396311, whole genome shotgun sequence.
ACCESSION   DAORTI010000208 DAORTI010000000
VERSION     DAORTI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24275_length_4501_cov_5.396311"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..591
                     /locus_tag="PLO70_16005"
     CDS             91..591
                     /locus_tag="PLO70_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HQZ76025.1"
                     /translation="MSKKWLAVYTRPRWEKKVNQLLAEKGLESYCPLNKVRRKWSDRV
                     KIVEEPLFKSYVFVKVTDEDRSTVRMTNGAVNFVYWNGKPAVIREKEINAIKRFLNEY
                     ENVEARKVELNVNQRVRITNGTFMDQEGKVLDVRHKMAKVAIDSLGYILVAYIDRSKL
                     TSALPK"
     gene            646..1431
                     /locus_tag="PLO70_16010"
     CDS             646..1431
                     /locus_tag="PLO70_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQZ76026.1"
                     /translation="MNFNRFLLLLALPLYLLSCKPQQVLPNYLENLSDTAGKGAVKIP
                     ELRIQKNDQLFIQVYSGSKKPEASDVFYNLPASQGTGQGAVTSGFLVDVSGNIDYPQL
                     GTIHAEGLTKLELAAEIKKRLTHPVELLADPSVIIRFLNFKVNVLGEVAKPGLINVPG
                     ERITILEAIGLAGDITQYGRKNTVKVIREIDGDRNIGTIDLSSKDLFESPYYNLLQND
                     VVMVEPSKRKAQSAEQALTAQKISFALTIVTVAATLANIFIRN"
     gene            1463..3871
                     /locus_tag="PLO70_16015"
     CDS             1463..3871
                     /locus_tag="PLO70_16015"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQZ76027.1"
                     /translation="MQDLDKNTINTNKSELWNLSFRDLFFKYVRFLPLFVISVALALL
                     AAFMYLRYAIPVYGVGGTMLIKSEQAGGRSDKFEELFVNDKAQNIQSEIEILKSKPLM
                     QRVVRSLNLQFTYYVEGKIKTVNIYKQSPFWIEAVQITDSSRSFTTTVKYLNDNEISI
                     NGGPATSFGKTFQNANGIFRIQKVQGASYSKDYSVIWKPVMAAAATYAAAISILPKTP
                     GTGILNVSMKTDNSYMGADIVNKLMEQYSNYSIEQKKLSSDQILSFIDARLEDYGRKL
                     DSVQTMYRDYQVKNNLINSEIQTGSYFENIAESDKTTNQQTIQFNVAGMIDDYLADKR
                     NEFGKIVVPSSLGLEDETLNELVGAYNKAQLERQQLLEANIPVNNPAVKQATGQIEKL
                     RVSIRENLKNIKSSVNTVINKYRQRSNISEEQLRAMPAKVIELAELKRQVETVQNLYK
                     FLQEKKEETAISRASTISSSGIIDKAFPNNNPVSPNRKAIQIMAVLIGLGLPALFIFV
                     GEVLNDKVSTRFDIEKLTQAPILGEIGHSYSDNTLVVSKTTRSMVAEQFRIIRSNLQY
                     VLNKKDKAVILTTSSFSGEGKSYISTNMGAVLALAGKKTVILEFDIRKPKVLSGLGIS
                     KGPGITNFLVGKTNNLQELIKPVPGHENLFVLGCGPIPPNPAELLLDPRLDEMFAWLR
                     ANYDIVLVDTAPVGMVSDAMTLSKFADCTLYLVRQGHTFKKQVALIDEFYQENKLPKV
                     SIIINDVKVKPGYGYYGYGRYGYGYGYGYGSYYEEETPPANFFERLLARFDVRKIFGS
                     RKRK"
     gene            3888..>4501
                     /locus_tag="PLO70_16020"
     CDS             3888..>4501
                     /locus_tag="PLO70_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ76028.1"
                     /translation="MRILLTGGAGFIGSNLVESLLQDERVSFVRVLDNLSTGALKNIE
                     EFQTHPKFEFTEGDICSYETCKAACHGMDIVSHQAALGSVPRSINDPLTTNNVNITGT
                     LNIFTAAKETGIKRIVYAASSSTYGDHPGLPKVENIIGNPLSPYAVTKYVNELYARVY
                     ASLYNMEFIGLRYFNIFGPKQNPGGAYAAVIPLFAEALLNNKPPT"
BASE COUNT         1474 a          795 c          981 g         1251 t
ORIGIN      
        1 tcagattttg caattaatat attgaaaatc aaatatataa ataatattta tgtgattgaa
       61 gtggcgaagc catgagcaaa attttttgaa ttgagcaaaa aatggttagc cgtttacaca
      121 cggccaaggt gggagaaaaa agtaaaccag cttcttgcag aaaaggggct ggagagttat
      181 tgcccgttga acaaagtaag acgtaaatgg agcgacaggg taaagattgt ggaggagcct
      241 ttattcaaat cctacgtttt tgtaaaagtg acggatgaag accggtcgac tgtaagaatg
      301 acgaatggcg ctgtcaattt tgtatattgg aatggaaaac cggccgtgat cagggaaaaa
      361 gagatcaatg ccataaaaag attcctgaat gaatatgaaa atgtggaagc ccgtaaagta
      421 gagctgaatg taaatcaacg ggtgcggatt accaacggaa ccttcatgga ccaggagggc
      481 aaagtgctgg atgtacgcca taaaatggct aaagtagcca ttgacagttt aggctatatt
      541 ttagtggctt acattgacag aagtaaatta acttcggcgc tgccaaaata aaaaccagac
      601 cttatttttt gcagcccgcc tgcacctatt aataaagacg aatcaatgaa ttttaaccgg
      661 tttttactac tattagcttt acccctttat ctcctttcct gtaaaccaca gcaggttctc
      721 cccaattatc ttgagaattt atctgataca gcaggaaaag gagccgttaa aatacccgag
      781 ttaagaattc agaagaatga ccagcttttt attcaggttt acagtggcag caaaaaaccc
      841 gaagcttcag atgtttttta taatcttcca gcttcacagg gtacaggcca gggggctgtt
      901 acgtcaggtt ttcttgttga tgtaagtggc aatattgatt atccccaatt gggtactata
      961 catgcagaag gccttacaaa actggagtta gcagcagaaa taaagaaaag attgacacat
     1021 ccggttgaat tattagcaga tccatctgtt attatccggt tcctgaattt taaagtaaat
     1081 gtattgggag aagtggcgaa gccgggactt attaatgttc cgggagaaag aataactata
     1141 ctggaggcca ttggtttggc cggcgatatt acacaatatg gcaggaagaa tacagtaaag
     1201 gtgatcaggg aaattgatgg cgacaggaac attggaacaa tagatctttc ttcaaaagac
     1261 ctgtttgaat caccgtatta caatttactt cagaacgatg tagtaatggt agagccttca
     1321 aaacggaaag ctcaaagtgc tgaacaggca ctcacggcac agaaaatatc ttttgcgtta
     1381 acgatagtaa ccgttgcagc cacattggct aatattttta taagaaatta atacctgcta
     1441 ttttttatta gcattttaat acatgcaaga tctagataag aacactatta atacgaataa
     1501 gagtgagctc tggaacctta gcttcaggga tctctttttt aaatatgttc gttttttacc
     1561 attatttgta atatcggtag ccttagcact gctggctgca tttatgtacc tgcgttatgc
     1621 aattcccgtt tatggtgtgg gcggtacaat gctgatcaaa agtgaacagg caggcggcag
     1681 aagtgataag tttgaagaat tgtttgtaaa tgataaagcc cagaatatcc agagcgaaat
     1741 agagatttta aagtcaaaac cactgatgca gagagtggtt aggagtctta atctgcagtt
     1801 tacttattat gtagaaggga aaattaaaac agttaatatt tataaacaat ctcctttctg
     1861 gattgaagca gtacagatca cagattccag ccgttcattt accaccacgg tgaaatattt
     1921 aaatgacaac gagatcagta ttaatggtgg tcctgctaca tcatttggaa aaacttttca
     1981 aaatgcaaac ggaattttca ggatacaaaa ggttcaggga gccagctata gcaaagacta
     2041 tagtgtaatc tggaaacctg ttatggctgc tgcagctact tatgcggctg caatatctat
     2101 attgccgaaa actcctggta caggtatttt aaatgtcagt atgaagacag ataattcata
     2161 tatgggtgct gacattgtca ataagctaat ggagcaatac agtaattata gtattgaaca
     2221 gaagaaattg tcttcagatc agatactgag ctttattgat gccaggctgg aagattatgg
     2281 acggaaactg gatagtgttc aaaccatgta cagggattac caggttaaaa acaacctgat
     2341 caattctgaa atacaaacag gtagttattt tgaaaatatt gcggaatcag ataaaactac
     2401 caatcagcaa accattcagt tcaatgtggc aggaatgatt gatgattacc tggctgataa
     2461 gcgaaatgaa tttggcaaaa ttgtcgttcc atcctcttta ggattagagg acgagacatt
     2521 aaatgaattg gtaggtgctt ataataaggc acaattagaa aggcagcaat tgctggaagc
     2581 aaacataccg gtgaataatc cggcagtaaa gcaagctact ggccagattg aaaagttgag
     2641 ggttagtatc agggaaaatt tgaaaaacat taaatcatca gttaatacag taattaataa
     2701 ataccggcag cgtagcaata tcagtgaaga gcaactaagg gcaatgccgg cgaaagtaat
     2761 tgaattggcg gagttaaaaa gacaggtaga aaccgtacag aatttgtata aattcttaca
     2821 agagaaaaaa gaagaaacag ctatttcaag agcctccact atttccagtt caggaataat
     2881 tgacaaagct tttccaaaca acaaccctgt atctcctaat agaaaggcaa tacaaataat
     2941 ggccgtattg attggcctgg gtttgccagc cctcttcata tttgttggag aagtactgaa
     3001 tgataaagta agcacccgtt ttgatataga aaaacttacc caggcaccga tattgggaga
     3061 aataggccat tcctactcag ataatacgct ggtagtgagt aaaaccaccc gtagcatggt
     3121 ggcagaacag ttcaggatta tccgttctaa cctgcaatac gttcttaata aaaaagataa
     3181 agcagtaatt cttaccacct cttcttttag cggagaaggt aaatcataca tatctaccaa
     3241 tatgggagca gtgctggcat tagctggtaa gaaaactgtc atccttgagt ttgatattcg
     3301 taaaccaaaa gtgttgtcag gattgggcat ttcaaaaggt ccgggtataa cgaatttcct
     3361 cgtgggtaaa acaaataacc tgcaggagct gataaagccg gttccggggc atgaaaattt
     3421 atttgtactt ggatgtggtc ctataccgcc taacccggca gaattattgc tggacccccg
     3481 gttagatgaa atgttcgcct ggttgagagc gaactatgat attgtattag tagatacagc
     3541 ccctgtaggt atggtaagtg atgccatgac cctgagtaaa tttgctgatt gcaccttgta
     3601 cctcgtaagg cagggtcaca cctttaagaa acaagttgcc ctgattgatg aattctacca
     3661 ggagaataaa ttacccaaag tctctattat cattaatgat gtcaaagtga agccgggtta
     3721 tggttattat ggctacggcc gctatggcta tggctatggt tatggctatg gcagctacta
     3781 cgaggaggaa acaccgcctg caaatttctt tgaaagacta ttggccaggt ttgatgtcag
     3841 gaaaattttt ggtagtagaa aacgaaaata aatatcccca atttccaatg cgtatattac
     3901 taacgggagg tgccgggttt atcggttcca atcttgttga aagtttgttg caggatgaaa
     3961 gagtatcatt tgtaagagtc ttagataatc tttctaccgg tgccctgaag aatatagaag
     4021 agtttcagac tcatccaaaa ttcgaattta ctgaaggtga tatatgcagc tatgaaacct
     4081 gtaaggctgc ctgccatggt atggatattg tttctcacca ggcggcgctc ggttcggtac
     4141 cccgttcgat caatgatccg cttaccacta acaatgtcaa catcaccggt acgctcaata
     4201 tttttaccgc cgcaaaagaa acaggcatta aaaggatagt atatgccgcc agttcttcta
     4261 cctatggcga tcatcccggt ttacctaaag tagaaaatat aatcggtaac cctctgtcgc
     4321 cctatgccgt taccaagtat gttaatgaat tgtatgcaag ggtatatgcc agtttgtata
     4381 atatggaatt tataggcctc cggtatttta atattttcgg gccgaaacaa aatcctggcg
     4441 gtgcttatgc agcagtgata cctttatttg cagaagcatt gttgaataat aaaccaccca
     4501 c
//
LOCUS       DAORTI010000209         4497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_24318_length_4497_cov_4.798739, whole genome shotgun sequence.
ACCESSION   DAORTI010000209 DAORTI010000000
VERSION     DAORTI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24318_length_4497_cov_4.798739"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..750)
                     /gene="ssb"
                     /locus_tag="PLO70_16025"
     CDS             complement(319..750)
                     /gene="ssb"
                     /locus_tag="PLO70_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQZ76029.1"
                     /translation="MIKMQVIGRLGKDCVVNTVNGKNVINFSVAHSEKYKDSQGSLQE
                     KTVWVDCAYWTDRTAVAQYLTKGTQVYAEGQPEARSFQRNDGTPGSSLSLRVREVQLL
                     GGKADSSGGPSSYQPNISSVSSGSPVTSPSDITEPVDDLPF"
     gene            complement(857..3421)
                     /locus_tag="PLO70_16030"
     CDS             complement(857..3421)
                     /locus_tag="PLO70_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQZ76030.1"
                     /translation="MKKWLFLVSFFVVSSCLYAQQLSYYLPESVSYNASIPKPNDIIY
                     HEVGEWHVTHDRLVNYMKAIAAAAPERVKLETMGFTYEGRPQVLLIITSKKNHDNLEN
                     IRQQHLQLSDPAKSASASIADMPIVVWIGHSIHGNEPSGANASLLTAYYLAAAQGKQI
                     DELLDKVVILVDPSFNPDGLQRFSTWANQHKSKNLVSDPNSREFNEVWPGGRFNHYWF
                     DLNRDWLPAVHVESQNRLKWFHLWKPNILTDHHEQGSNATFFFQPGVPSRVNPLTPEK
                     NQELTAKLGKFHAQYLDRIGSLYFTKENYDDFYYGKGSTYPDINGAIGILFEQASSRG
                     HLQETANGLLSFPATIKNQFVTALSTLEGAKTLRKEFLEYQRDFYKQAASRAAAYPVK
                     GYVFGDKNDLAKTFHFAEMLKRHQVEINTLPDTWKDAEFDKSSAYMVSLNQPQHSLIR
                     AIFDKNFEYKDSLFYDITSWTMPLAFGLPYKEITTAPFVTGEKVDALRMSKQTVAGGK
                     TEYAYLIQWDELYAPAALYELLNAGILAKVATNHFEILVDGVVKKFNYGTILVPVKLQ
                     QDNSETVFAKLSALTEKYRLKTVAVPSGNVNSGSDLGSSKFITITKPSIAMITGPGVN
                     ALDAGEVWHLLDQRMNMPATHLEPSIFNRVDLSKYNTLIMVGGNYNELNKEKLKSWVQ
                     GGGTLILTEEAVSWAAQIGIADVKFKRAKAAVDSTQKLTYIDREQVEGSQQVRGAIFG
                     ADVDLTHPLSYGYTQKTVSLFKGNKVFMEKSRNPYATPFYYGSKPLQSGWVSRENADV
                     IKNSAAVIVNTLGSGRVINIADNPNFRAFWLGGSKLFMNAIFFGRIIDAASGRSAE"
     gene            complement(3480..3725)
                     /locus_tag="PLO70_16035"
     CDS             complement(3480..3725)
                     /locus_tag="PLO70_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76031.1"
                     /translation="MRIPVLIKYVHPKYQIGLEDIRNAWLLENAAAPVKLKTIIWQGR
                     LAYRLPVTGKRISYLSLKKGLVRKELIIYTDLYLLPF"
     gene            3827..4096
                     /locus_tag="PLO70_16040"
     CDS             3827..4096
                     /locus_tag="PLO70_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76032.1"
                     /translation="MIDEIIDSLETKMYAVDHDMAYKEITSIEHGFSGSPKPFILVMK
                     ENKGKTKSLFNDQYFAHSKYTFFDSTLAETTVKMSDWTLVQVFEP"
BASE COUNT         1327 a          968 c          880 g         1322 t
ORIGIN      
        1 atagccgctg ctgttccgcc cacaccacct ccgatcacca atacctccgt gcttgttgtt
       61 ttcacctggc gttgggcaaa agcaaaaact ggaataagaa aaataatact gaatgcggtc
      121 tgcttcatat tgtcaagata caggaatcag gtaagaaaat aaaagagggt gacgcaaaaa
      181 aaaacaaatt tgtcacattg agtttatcga aatgcgaaca aatttgtttc ccaatcattg
      241 ccctatttcg gctttgctca atatgacaat gattggggct tttgagtcag cctcttataa
      301 agtagctgtt gaatactttt agaatggaag atcatccacc ggttcagtaa tatcactggg
      361 cgaagtaacg gggctgccgg aagatactga tgaaatgtta ggctggtaag atgacgggcc
      421 accactgctg tctgcttttc ctcccaataa ttgtacttcc cttacccgaa gtgaaagcga
      481 agaacccggg gtgccgtcat tgcgctggaa agaccttgct tccggctgac cttctgcata
      541 aacctgggta ccttttgtaa gatactgggc cactgctgtc cggtctgtcc agtacgcaca
      601 atccacccac actgtttttt cctgtaaaga accctggctg tccttgtatt tttctgagtg
      661 ggctacagaa aaattaataa catttttacc attgactgtg ttcaccacac aatctttacc
      721 aaggcgtccg ataacctgca ttttaatcat agtgctcaat ttttaaagtg acgtaattaa
      781 ttaagggagc taaaataaga ttaattaaca tcggaaaata acatcacccg ggccaatcaa
      841 aaaatgtgaa taaattctac tctgctgacc ggccacttgc cgcatcaatg atccttccaa
      901 aaaatatggc attcataaac aatttgctgc cacccagcca gaaagcccgg aagttgggat
      961 tgtcagcaat attgataaca cggccactgc ccaacgtatt cacaattaca gcggctgaat
     1021 ttttaattac atctgcattc tccctgctta cccatccgct ttgcaatggc ttgcttccat
     1081 aataaaatgg agtagcatac ggattcctgc ttttctccat aaatacttta ttccccttga
     1141 acaggctcac tgtcttttgt gtgtacccgt atgatagtgg atgcgtaaga tcaacatctg
     1201 ctccaaagat ggcacccctg acttgttgtg atccttctac ctgctcccgg tcaatataag
     1261 tcagtttttg tgtactgtcc actgcagcct ttgctctttt gaatttaaca tctgcaatac
     1321 caatctgcgc agcccagcta acagcttctt ccgtcagtat taatgtaccg ccaccctgca
     1381 cccatgattt cagtttttct ttgttgagct cattgtaatt acctcccacc atgatcagtg
     1441 tattatactt actaagatca accctgttga aaatggaagg ttccaggtgt gtggcgggca
     1501 tattcatccg ctgatccagc aaatgccata cttcacctgc atcgagtgca tttacaccgg
     1561 gaccggtgat catggcaatg gaaggttttg taatggtaat aaatttgctg ctgccaaggt
     1621 cactcccgct atttacattg cctgaaggaa cggcaactgt tttcaaccgg tatttttctg
     1681 tcagggcact caattttgca aacactgttt cactgttatc ttgctgtagt tttaccggta
     1741 caaggatggt tccgtaatta aacttcttta ccacaccgtc taccaatatt tcaaagtgat
     1801 tagttgccac tttcgccagg atacccgcat tcaataattc atataatgca gcgggtgcat
     1861 agagttcatc ccattgtata agataagcat actctgtttt gccaccagct acagtctgct
     1921 ttgacattct taaagcatcc actttttcac ccgttacaaa aggtgctgta gtgatctctt
     1981 tataaggcag gccgaaagcc aatggcatgg tccaggaagt aatatcatag aaaaggctat
     2041 ccttatattc aaaatttttg tcaaaaatgg ctcttatcaa actatgctgt ggctggttca
     2101 atgaaaccat ataagcactt gacttatcga actccgcatc tttccaggta tcgggtaatg
     2161 tatttatttc aacctggtgg cgcttcagca tttcagcaaa atgaaatgtc tttgccaggt
     2221 cgttcttatc accaaacaca tatcctttta ccgggtaagc agcagcacgg ctggcagcct
     2281 gcttgtaaaa atctctttgg tattccaaaa attctttgcg caaggtcttt gctccttcca
     2341 acgtagagag ggctgttaca aactggtttt taatggtagc cgggaagctg agcaagccat
     2401 ttgctgtttc ctgcaaatgc cccctggagg aagcctgttc aaataaaatt cctattgccc
     2461 cattgatatc aggataagtg gatcctttgc cataataaaa atcatcatag ttctctttgg
     2521 tgaaataaag agaaccgatg cggtctaaat attgtgcatg aaattttccc agcttcgcag
     2581 tcagctcctg gttcttttca ggagtcaatg ggtttacccg tgaaggaaca cccggttgaa
     2641 aaaagaaagt ggcattactt ccctgctcat gatggtccgt taaaatattc ggtttccaga
     2701 gatgaaacca ttttaaccgg ttctggcttt ctacatgcac agccggcaac cagtcacggt
     2761 tcaaatcaaa ccagtaatga ttgaaccggc caccgggcca cacttcatta aactccctgg
     2821 agtttggatc acttactaaa tttttactct tatgttggtt ggcccaggtg gaaaaacgtt
     2881 gtaatccatc aggattaaaa gatggatcaa ctaatatcac caccttatcc aataattcat
     2941 ctatttgttt tccctgtgca gctgccaggt aataagcagt caataaactg gcattggcac
     3001 cactgggttc atttccatga atagaatggc cgatccatac aacaatgggc atatcagcta
     3061 tgctcgcaga tgcagatttt gcaggatcac ttaactgcaa atgctgctgc cgtatatttt
     3121 ctaaattatc atgattcttt ttagaagtga tgatcaataa gacctgtggc ctgccctcat
     3181 aagtaaagcc cattgtttcc agtttcactc tttcgggtgc tgccgcggca atggctttca
     3241 tataattgac cagcctgtcg tgggtaacat gccactctcc tacttcgtgg taaataatgt
     3301 cgttgggttt gggaatggaa gcattatacg aaacactctc aggcaaataa taactaagtt
     3361 gctgtgcgta caagcaactg cttactacaa aaaaactaac caggaataac cattttttca
     3421 tacaggtgat tttagagagg gggaaattac ggaatcaggg ataaaatcaa tggacaatat
     3481 caaaaaggca acaggtacaa gtccgtgtaa ataataagct ctttacgaac caggcctttc
     3541 tttaaggaaa ggtagctgat ccgttttccg gtgacaggta aacggtaggc cagccgcccc
     3601 tgccaaatga tagtttttag ttttacaggt gctgctgcgt tctccagcaa ccaggcattt
     3661 cttatatcct ccaggccaat ttgatacttt ggatgaacat atttaataag taccggaatg
     3721 cgcataataa attgtttctg gcataaattt acgttgatta tcaattaaaa actatgccag
     3781 cagtggcgta tatttatgtg ttgtgtgaaa ttttagacag ttaactatga tagacgagat
     3841 aattgacagt ttagagacaa agatgtatgc cgtagaccac gacatggcct ataaagaaat
     3901 aacaagtatt gaacatgggt ttagtggatc gccaaaacct tttatattgg taatgaaaga
     3961 aaataaaggg aaaacaaaat ctttattcaa tgatcaatat tttgcacatt caaagtatac
     4021 atttttcgac agtacgttag cagaaaccac agttaagatg tctgattgga cattagtaca
     4081 agtttttgag ccgtaagttc gtaggcgaga ggctatttat acaatttcgg atccaaaagg
     4141 caaaattctt gaaatctcta acagttcatt ttctatcgaa gatgctatca ggaatgaaaa
     4201 ttttcagata ggtggtattt gcaatatttt aaagtttctg aagaaacttt ctagctatcc
     4261 tgattggacg tttttcgaag taatgaaaga aaaccaagaa ctaaaagcca cgttaaagaa
     4321 acttgatcac gagattgaag cattcattga aagaattcaa gaaattagaa aaggtataga
     4381 ataaaacttc acacaacaag gggtttggcg ttatggcgtc tttacgaaca tatcctgatc
     4441 gacataacat tactcagcga catatccaac tgacgaataa agcccagctt cagaata
//
LOCUS       DAORTI010000210         4477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_24482_length_4477_cov_6.853234, whole genome shotgun sequence.
ACCESSION   DAORTI010000210 DAORTI010000000
VERSION     DAORTI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24482_length_4477_cov_6.853234"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1876
                     /locus_tag="PLO70_16045"
     CDS             <1..>1876
                     /locus_tag="PLO70_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQZ76033.1"
                     /translation="IKAFKAEKEEAVDWKALARDERTWKIVGTVFLLISIFLFIAFAS
                     YLFTWKEDQAVAQQGFSALLDNDKPVANLLGRLGAVVSHFFIFKAFGVASFLICTFFF
                     VVGINLLFRRKVFSIWRNLKYVTVGLMVLSVSLSFVFAKNEFPFGGGVGDMLNRQLHG
                     AVGTVGTGAILILLAAGYFIWQFNPVFNLPKKKQEGVPEEADSEGIEEDIPVIAGQTV
                     NELYGGNTGQKTNTIKSDSGIVIKFPDETPLHDLKVIEKDEPLEEVIEKIEASQPTFE
                     KEIVSDILHTHELEIPAKPIKPAAEEFVQPVKEKPGTKWEEDDLQLEIKAVPEKAEEE
                     QQSVIENLPPYEPTLDLRDYKYPTLSLLETHGSEKIIQDTSELENHKNQIIATLRNYA
                     IEIQKISATIGPTVTLYEIVPAPGVRISKIKNLEDDIALSLAALGIRIIAPIPGKGTI
                     GIEVPNVRKTVVSMKTLLSSDKFQNNNFNLPIAIGKKIDNENFIVDLTSMPHLLMAGA
                     TGQGKSVGLNAILVSLLYKKHPSQLKFVLIDPKKVELSIYRHIDKHFLAKLPGEEDAI
                     ITDTKKVVHTLNALCIEMDNRYDLLKEAGARNIKEYNEKFIKRKLNPQKGHQYLPFIV
                     L"
     assembly_gap    1877..1976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1977..2395
                     /locus_tag="PLO70_16050"
     CDS             <1977..2395
                     /locus_tag="PLO70_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQZ76034.1"
                     /translation="IGKGDMLISHNGELTRLQCAFVDTPEVEQIVDFISSQRGYPQAF
                     LLPEYVDEKELEGRDFDMSDRDSLFEDAARLIVQNQVGSTSLLQRRMKLGYNRAGRLM
                     DQLEAAGIVGPSQGSKARDVLIKTEMELQQHLDTIG"
     gene            2533..3186
                     /locus_tag="PLO70_16055"
     CDS             2533..3186
                     /locus_tag="PLO70_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQZ76035.1"
                     /translation="MKKLYFLTTFSILFSTFSFAQTKNDPEAKKILDAVSAKFKTFST
                     VQANFTYKVENGAGKALSTKTGSILMKGTKYKLSFSGQEIFCNGTTVWNFDKAANEVT
                     VNNLDASSGMITPQKLFSNFYDKDFFYTLNGEKKVGAKTVQEIQMTPVDKSKSFHTVY
                     LQIDKAAKTIYSTKVLENAGNRYSYTVNSMKTNIPASDNQFVFDKSKYPGVIVEDLR"
     gene            complement(3235..4056)
                     /gene="kdsA"
                     /locus_tag="PLO70_16060"
     CDS             complement(3235..4056)
                     /gene="kdsA"
                     /locus_tag="PLO70_16060"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQZ76036.1"
                     /translation="MEKFLKELFSNQQYDENNFFLMAGPCVVESEELVMEVAVKVAGI
                     CKNMGIPYIFKASYRKANRTSASSFTGLGDEKALEILQKVRNKFSLPVVSDIHAHEEA
                     ALAAKYVDVLQIPAFLCRQTDLLIAAAQTGKVVNVKKGQFLSGPAMKFAAEKIRQAGN
                     DKVMLTERGTTFGYQDLVVDFRNIPWMKEHGTPVVMDVTHSLQQPNQATGITGGNPQL
                     IETIAKAAVATGADGLFIETHPNPSVAKSDGANMLKLDLLEDLLQKLVKIKKAIK"
     gene            4146..>4477
                     /locus_tag="PLO70_16065"
     CDS             4146..>4477
                     /locus_tag="PLO70_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ76037.1"
                     /translation="MIKEKILVIGASGQIGVELTLTLRKIYGNSNVIASDLREQNPLL
                     EGTGPYVSLDVMNKEMLHVQVIRQNITQIYLLAAILSATGEKNPGLAWNLNMQGLLNV
                     LDIAREEK"
BASE COUNT         1327 a          864 c          956 g         1230 t
ORIGIN      
        1 atataaaggc atttaaggct gaaaaggaag aggcggtgga ttggaaggcc ctggcccggg
       61 atgaacggac ctggaagatc gtgggtacag tttttttgct tatttcaatt ttccttttca
      121 tcgcatttgc atcctatctt tttacatgga aagaagacca ggcagtagcg cagcaggggt
      181 tttctgcctt gctggataat gataaacccg tggcgaacct gctgggacga ctgggggcag
      241 tcgtttctca tttcttcatt tttaaagctt ttggcgttgc ttcctttctt atctgtacat
      301 tcttttttgt agtgggaata aacctgcttt tccgcagaaa agtattttct atctggcgca
      361 acctgaaata tgtaaccgtt ggcttaatgg tactttcggt ttcattgtct tttgtttttg
      421 caaaaaatga atttcctttt gggggtgggg ttggagacat gcttaaccgc cagcttcacg
      481 gagctgttgg cactgtagga acaggagcca tattgatact gctggcagcg ggttatttca
      541 tctggcaatt taacccggtt tttaatttac caaagaaaaa gcaggaaggt gtaccggaag
      601 aagctgattc cgaaggaatc gaagaggata ttccggtgat agcaggtcaa acagtgaatg
      661 aactctatgg cgggaatacc ggccagaaaa caaataccat aaaatcagat tccggtatcg
      721 ttatcaaatt tcctgatgaa acaccactgc atgatcttaa ggtgattgaa aaagacgagc
      781 cgctggaaga agtaattgaa aaaattgaag caagtcagcc cacattcgaa aaggaaatag
      841 tgagtgatat tttgcatacc catgagttgg aaattcctgc aaagccaatc aaacccgcag
      901 cagaggaatt cgttcagccg gtaaaagaaa agccaggtac aaaatgggaa gaggatgatc
      961 tgcaactgga aataaaagca gtacccgaaa aggctgaaga agaacagcaa tcagttattg
     1021 agaatttgcc gccttatgaa ccgaccctgg atctgcggga ttataaatac cccactttaa
     1081 gcctgctgga gacacacggc agcgaaaaaa taatacagga taccagtgag ctggaaaatc
     1141 acaaaaacca gattattgct acactaagga attatgctat tgaaattcaa aagatcagtg
     1201 cgacgatcgg accaactgta acgctttatg aaatagtacc ggcacccggg gtgagaatat
     1261 cgaagattaa aaacctggaa gatgatattg cattaagcct ggctgccctt ggtattcgta
     1321 tcatagcacc catacccgga aaaggtacaa ttggtattga agtgccaaat gtgaggaaga
     1381 ccgtggtgag tatgaaaacc ctgttatcgt ctgataagtt tcagaataat aatttcaacc
     1441 tgccgatcgc cattggaaag aagattgata acgagaattt catagttgat ttaacaagca
     1501 tgccacacct gctgatggcc ggtgctacag gacagggtaa atcagttgga ttaaatgcta
     1561 tccttgtttc actattatat aaaaagcatc cttcgcagtt aaaatttgta ctgatagatc
     1621 ctaaaaaagt ggagctgagt atttaccggc atatagataa acatttcctg gccaaattac
     1681 ccggtgaaga agatgctatc attactgata ctaaaaaagt ggtccataca ctgaatgcat
     1741 tatgtattga aatggataac cggtacgatc tgctgaaaga agccggggca aggaatatta
     1801 aagagtacaa tgagaaattt ataaaacgaa aacttaaccc gcaaaagggg catcaatacc
     1861 ttccgtttat tgtactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgat
     1981 aggtaagggg gatatgctga tctcccataa cggggaactg acccgtttgc aatgcgcttt
     2041 tgtagacaca cctgaagtgg aacagatcgt cgactttatc agcagtcagc gggggtatcc
     2101 gcaggccttt ttattgcctg agtatgtgga tgaaaaggaa ttggaaggcc gggattttga
     2161 tatgtctgac cgggattcac tgtttgaaga tgctgcccgg ctgatcgttc aaaaccaggt
     2221 tggttccacc tccttgctgc agcgaaggat gaagctcggc tataacaggg cgggtcgcct
     2281 gatggaccaa ttagaagcag caggaattgt tggccccagc cagggaagta aagcaaggga
     2341 cgtattaata aaaaccgaaa tggaattaca acaacatctt gatacaatcg gttgatatct
     2401 acagcttcaa tccgttagtg gcttgttaag aaaagaacac aaatgcaacc caaacgagtc
     2461 attggcaatc ctattcctgt aattcattta tctttgcgcc gccgtaacag gtagccacac
     2521 aaacaccaaa taatgaagaa gttatatttt cttaccacat tcagtattct gttttccact
     2581 ttttcctttg cgcaaaccaa aaatgatcct gaggccaaga agatccttga tgctgtgagt
     2641 gcaaaattta agaccttttc tacagttcag gccaacttta cctataaagt agagaatggt
     2701 gctggtaaag cactatctac taaaacaggt tctattttga tgaagggtac caaatacaag
     2761 ctgagtttta gcggccagga gatattttgt aatggaacta ctgtatggaa ttttgacaaa
     2821 gctgcaaacg aagtgactgt taataacctt gatgcttcca gcggtatgat cactccgcaa
     2881 aaattgttct ccaattttta tgataaggat ttcttttata ccctgaatgg agaaaaaaag
     2941 gtaggtgcta aaacagttca ggaaatacag atgacacctg ttgataagag caaatctttc
     3001 cataccgtat acctccagat agataaagcg gcaaagacca tatacagcac caaagtactg
     3061 gaaaatgcag gtaacaggta ttcttatacg gtgaactcaa tgaagacaaa tatccctgct
     3121 tccgacaacc agtttgtttt tgacaaaagc aaatacccgg gagtgatcgt cgaggatctg
     3181 agatgatctt tataaataac attgaaaaag gggttcttta accgggatcc ctttttattt
     3241 tatcgccttt ttgatcttta ctaatttttg caacaagtct tctaacaagt ctaatttcag
     3301 catattagct ccatcacttt tagcgactga tggattggga tgggtttcaa taaacaaacc
     3361 atcagctccg gtagcaactg cagcttttgc tattgtttca atcaactggg gattgccacc
     3421 cgtgatacca gttgcctggt tgggttgttg aagagaatgt gttacatcca tcaccaccgg
     3481 ggttccatgt tccttcatcc agggaatatt cctaaaatca acaaccaggt cctggtaacc
     3541 aaaagttgtc cctctttctg tcagcatcac tttatcatta cctgcctgcc tgatcttttc
     3601 tgctgcaaac ttcatggcag ggccgcttag gaattgccct ttttttacat tcactacttt
     3661 tcctgtttgt gcggctgcga tcaataaatc tgtctggcga cataagaatg caggtatctg
     3721 taatacatcc acatattttg cggcgagtgc tgcttcttca tgcgcatgaa tatcggaaac
     3781 aacgggaagg gagaacttat ttcttacttt ttgcaaaatt tccaaggctt tttcatcacc
     3841 cagtcctgta aaggaagagg cgctggtacg attggcttta cggtaagatg ctttgaaaat
     3901 ataagggatg cccatgtttt tgcaaatacc tgcaactttt actgcaactt ccattaccag
     3961 ttcctcgctt tccaccacac aggggcccgc catcaggaag aaattatttt catcatattg
     4021 ctgatttgaa aatagctctt tcaaaaattt ttccatgccg caaaaatagt tttaagttct
     4081 gagttttggt ttttaagttg gaatttgact tttgttattt aatctttgct ttacaatagc
     4141 ttgctatgat aaaagaaaaa atactggtta tcggagcctc tggtcagata ggagtggagc
     4201 tcacattaac ccttcgtaaa atatacggta attcaaatgt cattgcttcc gacctgcggg
     4261 aacaaaatcc cttactggaa ggtaccggcc cctatgtttc attggatgta atgaataaag
     4321 aaatgctgca tgtgcaggta atacggcaga atattaccca gatatacctg ttagctgcta
     4381 ttctctcggc caccggtgaa aagaatcccg gtcttgcctg gaacctgaat atgcagggcc
     4441 tgttgaatgt attggatatt gcaagagaag agaaatt
//
LOCUS       DAORTI010000211         4474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_24508_length_4474_cov_7.819869, whole genome shotgun sequence.
ACCESSION   DAORTI010000211 DAORTI010000000
VERSION     DAORTI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24508_length_4474_cov_7.819869"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1745)
                     /gene="ggt"
                     /locus_tag="PLO70_16070"
     CDS             complement(21..1745)
                     /gene="ggt"
                     /locus_tag="PLO70_16070"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQZ76038.1"
                     /translation="MQKKITSFLFLLLILAACKPAQKNTAAPAINAYNYSGVKKVVAE
                     NGAVVSAHPLASKVGVEILKMGGNAVDAAIATQLALAVVYPNAGNLGGGGFMVARLSN
                     GETVALDYREKAPGQAHRDMYVETDGKANTDKSQLGHLSSGVPGTVSGLFASHKYGKL
                     AFEKLIQPAIELAEKGFTISASEARSLNYLQNDLKKYNTVTPVFVKNTPWKQGDTLLQ
                     TDLANTLKRMQQKGAAGFYEGETARLIVEEMKRGNGIISLEDLKNYQAKFRTPHSFLY
                     KGYKIVSMPMPSSGGVLIHQMMKMIEERNIAAMGFHSLQAVQLMTEVERRAFADRGEY
                     MGDADHYKVPVDMLTNEVYLQERMKNYDPAKATPSVMVQPGTVLRTESEETTHLSVID
                     KEGNAVAVTTTLNNSYGSRTVVGGAGFFMNDEMDDFSIKPGVPNMYGAIGGEANAIKP
                     GKRMLSSMTPTLVLKDDKPYIVVGTPGGTTIPTQIFQTLVNILEFNMSAEEAVYKPKF
                     HHQWLPDEIMIEKGFPLAVKEALQKMGYSFSKERSGIGRTEIIMVLPNGKFEAVADNR
                     GDDAAEGW"
     gene            1857..4382
                     /locus_tag="PLO70_16075"
     CDS             1857..4382
                     /locus_tag="PLO70_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQZ76039.1"
                     /translation="MFRKALFSLIALLFLLVSNAQLKSPEEFLGYKVGTRYTPHFKIV
                     NYFNHVAANASAMVKLQQYGETNEGRPLLLTFISSADNIKNLEQIRMNNLRLANLSKD
                     KMAAMEENAPAIVWLSYNVHGNETSSSEASMLTIYELVNPAQLKTKEWLKNTVVIIDP
                     CLNPDGRDRYINWFNSVVGKTFNPQRLAREHREPWPGGRTNHYNFDLNRDWAWQTQLE
                     SQHRIKIYNQWMPQVHVDYHEQGINEPYYFAPAAEPFHEVITPWQREFQTTIGKNHAK
                     YFDANGWLYFTKERFDLLYPSYGDTYPTYNGSIGMTYEQGGIGAGLGIINEDDDTLTL
                     VDRAQHHFTTSLSTVEISSLHANKLVKEFRKFFNDAVSAGMGEHKTYVVKYDFRQQER
                     IEKLVELLDKNGIQYTAGNGATSKGINYITGKEETFTASANDIVVSVLQPRSAMVKVL
                     FEPKSKLSDSATYDITAWSLPYVYGLNAFASKDKIAGAGSYTVQTKVNNAETEYGYVI
                     PWSGVKAAKTVGHLLQKGILLRFAEQAFEVNGNKFDRGAVIVLKTANKKFGKDLWNIV
                     RETANADGVQLYPVSSGFVDKGFDFGSDMVHPLKAPSVVLLTGEGIGSNAAGEIWHFF
                     DRQLDYPVTLINSSDISRVNWNDIDVLIMPDGNYRFLTNKEELEAFKSWINKGGRVVA
                     LESAVAQLAKAEMGIRQKKPEEADKKEKSENYDALKEYQARERDAITGFTPGSIWKVK
                     LDNSHPLAFGYPDYYYTLKMDDNIYEFMKEGGWNVGVIKKENQVAGFVGAGLQPRLKD
                     GLLFGVQQMGGGSISFLTDNLLFRNFWENGKLMFCNAVFLVGQ"
BASE COUNT         1264 a          939 c          978 g         1293 t
ORIGIN      
        1 tcaaacctca aactctacac tcaccatcct tccgccgcat catccccccg gttatcagca
       61 acggcctcaa atttaccatt gggcaatacc attattattt ctgttcgccc gataccactt
      121 cgttctttgc tgaaactata acccattttt tgcagtgctt cctttacagc cagcggaaat
      181 cctttttcaa tcatgatctc atccggtagc cactggtggt gaaattttgg tttgtatact
      241 gcctcttccg cactcatatt gaattcaaga atattgacca gtgtctgaaa gatctgcgtg
      301 ggaatggtag tgccaccggg agtaccaacc acgatatatg gcttatcatc ttttaatacc
      361 agtgtaggtg tcatggagct gagcattctt ttgccgggtt tgattgcatt ggcttcgccg
      421 ccaatagcac catacatatt gggcacaccg ggtttgatac tgaaatcatc catctcgtca
      481 ttcataaaaa aaccggcacc gcccactaca gtccggctgc catatgaatt attgagagtg
      541 gtggttactg ctactgcatt cccttcttta tcaatcacac tcaggtgcgt agtttcttca
      601 ctttctgttc ttaacacagt gccgggttgt accattacac ttggcgttgc ttttgccgga
      661 tcataatttt tcattctttc ctgcaaatac acttcgttgg taagcatatc caccggcact
      721 ttgtaatgat ctgcatcgcc catgtattcg ccccggtctg caaaagccct tctttccact
      781 tcggtcatta actgaaccgc ctgcagcgaa tgaaaaccca tggctgcaat atttctctct
      841 tcgatcattt tcatcatctg gtgtatcagc actccgccac tgctgggcat gggcatgcta
      901 actattttgt atcctttgta tagaaaggag tggggtgttc tgaactttgc ctggtagttt
      961 tttaagtctt ctaaactaat gataccatta ccccttttca tttcttctac gatcagcctg
     1021 gcggtttcac cttcataaaa acccgcagcg cctttttgct gcattctttt taaggtattg
     1081 gcaagatcgg tttgcagcag ggtatcgccc tgtttccacg gcgtattttt tacaaatacc
     1141 ggtgttactg tattgtattt ttttaggtcg ttctgtaagt agttaagtga cctggcttct
     1201 gatgcagaaa tagtaaatcc tttttctgct aattcaatag cgggttgtat cagtttttca
     1261 aatgccagtt taccatattt atgcgatgcg aaaaggccgg atactgttcc gggtacgccg
     1321 gatgaaagat gtcccaactg gcttttatcg gtattggctt ttccatctgt ttccacatac
     1381 atatcccggt gagcctgtcc cggtgctttt tcccggtaat ccaatgccac tgtttctccg
     1441 ttactcaatc ttgccaccat aaaacctccg ccacccaggt taccggcatt gggatataca
     1501 acagcgagtg ccagttgtgt agcaatagct gcatctacgg cattaccgcc cattttcaag
     1561 atctccacac ctactttgct ggcgagtgga tgagctgata ctacagcccc gttttcagcc
     1621 actacttttt ttacaccgga ataattatag gcattgatgg caggagccgc tgtattcttt
     1681 tgtgcaggct tgcaggccgc caatatcaac aacaggaata aaaacgaagt aatttttttt
     1741 tgcatgtggc agattttaaa gtaaataaag ctacataaac cggtgcattt aacttcaatc
     1801 aaaatttgcg gtaattaaag ctgcctttgt taacttgctg cactaaaaat tcaactatgt
     1861 tccgtaaggc gctgttctct ttaattgctt tattattttt actggtttcc aatgcacagc
     1921 taaaatcccc ggaagaattc ctgggataca aagtaggcac ccgttacacc ccgcatttta
     1981 agatcgtcaa ttattttaat catgtcgctg caaatgcttc tgccatggtc aaattgcagc
     2041 aatacgggga aaccaatgaa ggcaggcctt tgttactgac ttttatttct tctgccgata
     2101 atattaagaa cctggagcag atacggatga acaatctcag gctggctaat ctttcaaaag
     2161 acaagatggc tgctatggaa gaaaatgcac cggcaattgt atggttaagt tataatgtcc
     2221 acggcaatga gacatcctct tctgaggcat ccatgctcac tatctatgaa ctggtaaacc
     2281 ctgcacagtt aaaaacaaag gaatggttaa agaataccgt tgtgattatt gatccttgcc
     2341 tgaacccgga tggcagagac cgttatatca attggtttaa ctccgtagtg ggtaaaacct
     2401 ttaacccgca gcgcctggca agagaacacc gggagccctg gccgggagga aggaccaatc
     2461 actataattt tgacctgaac cgtgactggg cctggcaaac acagctggaa agccagcatc
     2521 gtattaaaat ctataatcaa tggatgccgc aggtgcatgt ggattatcat gagcagggaa
     2581 tcaatgagcc ttattatttt gccccggctg ctgaaccttt tcatgaagta attacaccgt
     2641 ggcaacggga gtttcaaacc actattggaa aaaatcatgc aaaatatttt gatgccaatg
     2701 gctggttgta ttttaccaaa gaaagatttg acctgttata tccttcctac ggtgatactt
     2761 atccaactta taatggcagc ataggaatga cctatgaaca aggtggtatc ggcgccggac
     2821 ttggtattat taatgaggat gatgacaccc ttaccctggt agacagggct caacatcact
     2881 ttaccacctc actcagtaca gtggagatct cttctctcca tgcgaataaa ctcgtaaagg
     2941 aattcaggaa atttttcaat gatgctgtaa gtgccggcat gggagaacac aaaacgtatg
     3001 tagtaaaata tgatttccgg caacaggaaa ggattgagaa actggttgaa ctgttagata
     3061 aaaatggcat tcagtacaca gccggaaatg gtgcgacatc caaaggaatt aattatataa
     3121 ccggtaagga agaaacattt acggcaagtg ccaatgatat tgtagtatct gtattgcagc
     3181 cccgttcggc aatggtaaaa gttttgtttg agccaaaatc taaactgtca gattctgcta
     3241 cctatgatat cactgcctgg tccttaccct atgtgtatgg acttaacgca tttgcatcaa
     3301 aagataaaat tgcaggagca ggaagttata ctgtccaaac taaagtaaat aatgccgaaa
     3361 cagaatatgg gtatgtaatt ccatggagtg gtgtaaaagc agcaaagacc gttggccatt
     3421 tattgcagaa aggaatactg cttcgttttg cagaacaggc atttgaggta aacggaaata
     3481 aatttgaccg tggtgctgtt atcgtattaa aaacagcgaa taaaaaattc ggaaaggatc
     3541 tctggaatat agtgagggaa acagcaaacg ctgatggtgt acagttgtac ccggtgtcaa
     3601 gcggtttcgt agataaaggt tttgatttcg gaagcgatat ggttcatccg ctgaaagcac
     3661 ccagcgtagt attattgaca ggtgaaggga ttggcagtaa tgctgccggt gaaatctggc
     3721 atttctttga ccggcagctg gattacccgg ttacacttat aaatagtagt gatatcagcc
     3781 gtgtcaactg gaatgatatt gatgtattga tcatgccgga tggtaattac cggttcttaa
     3841 ctaacaaaga agagctggaa gcatttaaat cctggatcaa taagggagga agagtggtgg
     3901 cactggagag tgcagtagca caattggcga aagcagaaat gggtatcagg caaaagaaac
     3961 cggaggaagc agataaaaaa gaaaaaagtg aaaactatga tgcattaaaa gagtaccagg
     4021 cccgtgaaag agatgccatt acgggattca cccccggttc tatctggaaa gtgaagttgg
     4081 acaactcgca tccgctggct tttgggtacc cggattatta ttacacctta aagatggatg
     4141 ataatattta tgagttcatg aaagaaggtg gctggaatgt tggggtgatc aaaaaggaaa
     4201 accaggtagc aggttttgtt ggagcaggat tgcaaccccg gttgaaggat ggattgttat
     4261 ttggagtgca gcaaatggga ggcggcagta tcagcttcct gaccgataat cttctgttcc
     4321 gcaacttttg ggaaaacgga aagctgatgt tttgcaatgc cgttttttta gtgggtcaat
     4381 aataaaataa ttgacagaaa gaagaggctt tctcaaaagc aaacaaattt gtcacattga
     4441 gtttatcgaa atgcgtacaa atttgtttgc caat
//
LOCUS       DAORTI010000212         4374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_25374_length_4374_cov_8.627460, whole genome shotgun sequence.
ACCESSION   DAORTI010000212 DAORTI010000000
VERSION     DAORTI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25374_length_4374_cov_8.627460"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLO70_16080"
     CDS             <1..183
                     /locus_tag="PLO70_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76040.1"
                     /translation="LSYVLSYAEVSVIIPGIRTKEHVKGNTSGLFQLESDDMKLIEKM
                     GASQFMPIMDMILRQG"
     gene            complement(205..1743)
                     /gene="gltX"
                     /locus_tag="PLO70_16085"
     CDS             complement(205..1743)
                     /gene="gltX"
                     /locus_tag="PLO70_16085"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQZ76041.1"
                     /translation="MSKKVRVRFAPSPTGGLHLGGVRTVLYNYLFAKKNDGDFIVRIE
                     DTDQNRYVEGAEEYIFDTLKWCGLEPDESVQHGGAFGPYRQSERKESYRQYAEQLVKE
                     GYAYYAFDTPEELEKMRQTTRAIGDKTEQSHTQQYDHSIRMKMRNSLALTTAETQKML
                     AEGTKHVIRIKVPEKETVSFTDMIRGEVSFDTSTVDDKVLLKADGMPTYHLAVVVDDF
                     LMNISHAFRGEEWLPSAPVHILLWKYLFGLENMPQWAHFPLILGPNGKLSKRDGAKYG
                     FPVFAMRWNDPKTGELTEGFKEKGFLPEAFINLLALLGWNDGTEQEIFSKKELIEKFS
                     MDRVHSAGAKFDYEKAKWFNHEWIKRFEASNLRFEVGGLLKQNGFVVANDAFLDKVIA
                     LVKDRCTLLTDFVQQSSFFFKTPEVLDIDAVKPKWNEAKQLFFAELIRNYELSSGWDA
                     HTLENNFKEIAAVNDLKPGELMLPFRIMLVGGKFGPGVFDIAEMLGKEETIKRITMAI
                     KVFQ"
     gene            1813..2445
                     /locus_tag="PLO70_16090"
     CDS             1813..2445
                     /locus_tag="PLO70_16090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ76042.1"
                     /translation="MQKYFVKTPWWLKQLYSSRIWSIDTNEKKIYLTFDDGPHPTITP
                     FVLGELKKYGAKATFFCIGKNVVSYPGVYQQILDEGHRTGNHTQNHLNGWQTVNDIYF
                     SDIEEATLYINSDLFRPPYGRIRSSQAKYIRRAMDKETAKIVMWDVLSGDFDKSTTPE
                     QCLKNVVEKTNDGSVIVFHDSVKAWEKLSYTLPRILEHFSRKGFTFSAIS"
     gene            complement(2555..3442)
                     /locus_tag="PLO70_16095"
     CDS             complement(2555..3442)
                     /locus_tag="PLO70_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76043.1"
                     /translation="MKIRFITTALLPLLLFFSLLTISSCQKEDSQNGTVEEQEIEASK
                     ASSEADSEAEIVFNGIFDDAMGVNDEVGVGGTGVFGRLNVCPVVTVTRLNPPDLFPVR
                     ILMDFGTNGCIGPDGHFRKGKVITTYTNRLMIPGAIATTIFDGFYFDSTKVEGTHKIT
                     NTSATNIARRFTVNVIEAKLTKPNGNFIEWNSEKVITQLEGLSTPAPMDDIFKIEGGS
                     RGRVKRGALLVGWQSTILEPLVKRFNCRWIVRGVIRTVRLNATTNSPWIAVLNFGNGN
                     CDNLAVITINGVSYQITLP"
     gene            3558..4331
                     /locus_tag="PLO70_16100"
     CDS             3558..4331
                     /locus_tag="PLO70_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQZ76044.1"
                     /translation="MTLIDTHSHIYLPEFDSDIAEMFERADKEGVIKILLPAIDSETH
                     LQMLALVAKDPGRCCCMMGLHPCSVKENFREELKTARHYLDKRNFVAVGEIGLDFYWD
                     KTFTTEQYAAFHEQIEWAMEFDIPIVIHSRNSTAECIKVVAEHQKGKLKGVFHCFSGN
                     IQQAEQIIDLGFYLGIGGVLTFKNSGLDKVMESISLDHIVLETDAPYLAPVPFRGKRN
                     EPSYLKYIVEKLAVLKNRSLEEVAVITTANAEKLFSMAK"
BASE COUNT         1246 a          883 c          889 g         1356 t
ORIGIN      
        1 ttaagttatg tactgagtta tgctgaagta tctgttataa ttcccggtat caggacaaag
       61 gaacatgtga aaggaaatac atccgggttg tttcaattag aaagcgatga catgaaattg
      121 attgaaaaaa tgggggcttc tcagtttatg ccaattatgg atatgatact gcggcaagga
      181 taatggaaat caaacagagc tcaattattg gaatactttt attgccattg ttattctttt
      241 aatagtctct tcttttccca gcatttctgc gatatcaaaa acaccaggac cgaattttcc
      301 acccaccagc ataatgcgga aggggagcat caattcaccc ggtttcaggt catttacagc
      361 tgctatttct ttaaaattat tttccagtgt atgagcatcc catccggagg ataattcata
      421 gttgcggatc aattctgcaa aaaataattg tttggcttca ttccattttg gctttactgc
      481 atcaatatcc agtacttctg gtgttttaaa gaagaaagaa gattgctgta caaaatcagt
      541 gagcagtgtg cacctgtcct tcaccagcgc aatcactttg tccaggaatg cgtcatttgc
      601 aacaacaaaa ccattctgtt tcagcagccc accaacttca aacctcaaat ttgaagcttc
      661 aaaccttttg atccattcgt ggttgaacca tttagctttt tcataatcga actttgctcc
      721 tgcgctgtgc accctgtcca tcgaaaattt ttctatcagt tcttttttgg aaaagatttc
      781 ctgttctgtc ccgtcattcc agcccaataa cgccaacagg ttgatgaatg cttccggtaa
      841 aaatcctttt tctttaaatc cttcagttaa ctcaccggtt ttagggtcat tccatctcat
      901 ggcaaaaaca gggaagccat atttggcgcc atctctttta ctcagttttc cattaggtcc
      961 gagtattaac gggaagtgtg cccattgcgg catattttcc aggccaaata aatatttcca
     1021 aagtaaaata tggactggag cggaagggag ccattcctca ccacgaaaag catgagagat
     1081 gttcatcagg aaatcatcca ctaccactgc caggtgataa gtaggcatac catcagcctt
     1141 taataatact ttgtcatcta cagttgaagt atcaaaactt acttcccctc ggatcatatc
     1201 agtgaaagag acagtttctt tttccggtac tttaatacgg attacatgtt ttgtcccttc
     1261 tgcaagcatt ttttgtgttt ctgcagttgt cagtgccagc gaattccgca ttttcattcg
     1321 gatagaatgg tcgtattgtt gagtgtggct ttgttctgtt ttatctccga tagctctcgt
     1381 agtctgtcgc attttttcca gttcttcggg ggtatcaaat gcgtaatagg cgtagccttc
     1441 ttttaccaat tgctccgcat attgacgata gctttccttt ctttcgcttt gccggtaagg
     1501 tccgaatgcc ccgccatgtt gtacactttc atcaggttcc agtccacacc atttcagggt
     1561 gtcaaaaata tattcttccg caccttccac atagcggttc tggtcagtgt cttctatccg
     1621 tactataaag tcaccatcat tttttttggc aaataaataa ttgtataaaa cagttcttac
     1681 accacctaag tgcaggccac ccgttggact tggcgcaaaa cgaactctta cttttttact
     1741 catacagtgc aaagatagac cgctggttac agtgattaaa atgattttac tgggtaagct
     1801 atctttgtac caatgcagaa gtattttgtc aaaactccct ggtggctgaa gcagttatat
     1861 tcgtcccgga tctggagtat tgatacaaac gaaaagaaga tatacctcac ttttgatgat
     1921 ggcccgcatc ccacaatcac tccctttgta ctcggtgaat taaaaaagta tggtgcaaaa
     1981 gcaacttttt tttgtatcgg gaaaaacgta gtcagttacc cgggtgttta tcagcaaatt
     2041 ttggatgaag gtcaccggac cggaaaccat acacaaaacc atctgaacgg atggcaaacc
     2101 gtcaatgata tttatttctc cgatattgaa gaggcgacat tatatattaa ctcagatctt
     2161 ttccgaccac cttatggtag gattcgttcc tctcaggcaa agtatatcag aagagcaatg
     2221 gataaggaga cggcaaaaat tgtaatgtgg gatgttttaa gcggtgattt tgataaatca
     2281 acaactccag agcagtgttt gaaaaatgta gttgaaaaaa ctaacgatgg ttcggtcatc
     2341 gtttttcatg atagtgtgaa ggcatgggag aagttaagct atactttacc ccggattctg
     2401 gagcatttta gtcgaaaggg atttactttt tcagctattt cctgatttgt tagtgcttta
     2461 cctccccaaa aaagttaggg cctgggtaga aaccctggcc cgtttttaat ccgtacccta
     2521 tgaaaactgg tttaaaggta taataatttt ttaattacgg caaagtaatt tggtaagaaa
     2581 ctccgtttat tgttatgacg gccaggttat cacagtttcc gttgccgaag ttgagcactg
     2641 ctatccaggg actgttagtt gtagcattca gacgaacagt cctgattaca cctcttacaa
     2701 tccagcggca gttaaatctt ttcaccagtg gttcgaggat agttgattgc cagccaacga
     2761 gtaaggcacc cctttttacc ctgccgcggg agccgccttc aatcttgaaa atatcatcca
     2821 tgggagctgg ggtagacaat ccttccagct gagtaatcac tttttcgctg ttccattcaa
     2881 taaagttgcc attgggtttg gtaagctttg cttcaattac attcacagta aaccgtctgg
     2941 ctatattggt agcacttgta ttggtgatct tatgcgtgcc ttcaactttt gtactatcga
     3001 agtagaaacc atcaaatata gtggtggcaa tggctccggg aatcatcagg cgattggtat
     3061 atgtagtgat caccttgcct tttctgaaat gcccatccgg tccaatacat ccattagtac
     3121 caaaatccat cagaattctg actggaaaaa ggtctggggg gttgagcctg gtcacagtaa
     3181 caacagggca aacgttcaaa cgaccaaata cacctgttcc accaacacca acttcatcgt
     3241 ttactcccat cgcatcatcg aaaatgccat tgaaaacgat ttcggcctca gaatctgctt
     3301 cactggaagc tttagaagcc tcaatttcct gttcttctac ggtgccgttc tggctatctt
     3361 ctttttggca ggagctgatt gtaagcaggc tgaaaaatag caaaagaggc aataatgcag
     3421 tcgtaataaa cctgattttc ataaacttat agttttaaga gttgattatt aacggggata
     3481 ggatggattt tattggtaaa agtttaaagg tttcttaaag ttttttactg agttttacag
     3541 gctggaaaac atctaaaatg accctaatag acacccattc tcatatttat ttgccagaat
     3601 ttgattcgga cattgctgaa atgtttgaaa gagctgataa agaaggagtt ataaaaattt
     3661 tattacctgc tatcgattct gagacgcatt tacaaatgct tgcgttggtg gctaaagatc
     3721 ctggtcgatg ctgttgtatg atgggcttgc atccctgttc ggtgaaagag aatttccggg
     3781 aggagctaaa aacggcccgg cattacctgg ataaacgaaa ttttgttgca gtaggtgaga
     3841 tcggacttga tttttactgg gataaaacct ttaccacaga acaatatgct gcttttcatg
     3901 agcaaataga atgggcaatg gaatttgaca ttcctatagt gattcattcc cgcaactcca
     3961 cggccgaatg tattaaggtc gttgcagaac atcaaaaagg taagttgaaa ggagtttttc
     4021 attgtttttc gggcaatatt caacaggcag aacaaatcat tgaccttggt ttttatctgg
     4081 gtataggcgg ggtattgact ttcaaaaatt cagggttgga taaggtaatg gagtctataa
     4141 gtcttgatca tattgtactg gaaacagatg ccccctacct ggcacctgta cctttccgtg
     4201 gtaaaagaaa tgagccttct tatcttaaat atatagtaga aaaattggcg gtattaaaaa
     4261 atcgatcgtt ggaagaagta gctgttataa caactgctaa tgcagaaaaa ttattcagca
     4321 tggccaagta aaaatccctc aatatgaggg attctttttg cggagactga ggga
//
LOCUS       DAORTI010000213         4331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_25785_length_4331_cov_7.118803, whole genome shotgun sequence.
ACCESSION   DAORTI010000213 DAORTI010000000
VERSION     DAORTI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25785_length_4331_cov_7.118803"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLO70_16105"
     CDS             complement(<1..615)
                     /locus_tag="PLO70_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQZ76045.1"
                     /translation="MAGIADLIGNTPLVELRKLNPNPNVTIYAKLEGNNPGGSVKDRP
                     ALNMIRSALERGDIKPGNRLIEATSGNTGIALAMIARLYDLDIELVMPSSSTRERTLT
                     MEAFGATVTLLESIEVCRDYADEKAACGEAFILNQFANPDNYIAHYKTTGPEIWKDSN
                     QSITHFVSSMGTTGTIMGCSRYLKEKNPSIQIVGCQPTEESSIPG"
     gene            complement(621..1427)
                     /locus_tag="PLO70_16110"
     CDS             complement(621..1427)
                     /locus_tag="PLO70_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HQZ76046.1"
                     /translation="MNQAFYDHIYSQQQAIEAVPPNEEIAGCVLKLFCLLFPERAECN
                     MDSAEEVEAAFVKMKAELIRIFNATKACDQCNNVEKANAFFNVIPDLYAVLNTDITAI
                     LEGDPAAHTRFEIIRAYPGFYAIFFYRVAHQLYLLDIPLLPRIMTEWAHSKTGIDIHP
                     GAKIGNHFFIDHGTGIVIGETCIIGNHVKLYQGVTLGALSVDKSMAFTKRHPTVEDHA
                     IIYSGATILGGETVIGHHSIIGGNVWLTKTVPPHSVVYHRPDIEVVEKIK"
     gene            complement(1557..2624)
                     /gene="fbaA"
                     /locus_tag="PLO70_16115"
     CDS             complement(1557..2624)
                     /gene="fbaA"
                     /locus_tag="PLO70_16115"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQZ76047.1"
                     /translation="MKKYRAGVLFGDELEALYNDARDNQFALPAVNTIGTNTINATLE
                     TAAKVNSPVIVQFSNGGAQFIAGKGMPNDNLQGNISGGISGALHIHNVARYYGVPVVL
                     HTDHAAKKWLPWISGLIDAGEQFHKEKGQPLFSSHMLDLSEEPIEENIHTSVEFFKRM
                     QPLGMSIEIELGVTGGEEDGVDNSDVENSKLYTQPEDVAYAYRELSKVGKLFTVAAAF
                     GNVHGVYKPGNVELRPVILNNSQVHIEKEFKTAPKPVYFVFHGGSGSPQHQIREAIGY
                     GAIKMNIDTDLQWAFWEGILENYKKNEAYLQGQLGNPEGDDKPNKKYYDPRVWLRKGE
                     ESFSKRLELAFEDLNCINRNA"
     gene            complement(2729..3844)
                     /locus_tag="PLO70_16120"
     CDS             complement(2729..3844)
                     /locus_tag="PLO70_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76048.1"
                     /translation="MRFIDNQNGRETKEGDKPSRKKPMYPVNDKLRNYLKNRGREVKL
                     PVTYNNLLNFTWSTPIKDKHGNDTYWEKTSYDSRDWDYIRQGLVKIYAILKTEGDFSF
                     ISHLDVARIDYCAFGNSNPFRIRIVNKFNDNYDHYYIKIADASRIYGLELEHILSPNR
                     ITFFTCDNTLVEEHIPGIPGDVFIKQMMDSPETNKIRLAKEFVKFNERCFVRLLGDMR
                     SYNFVIGITPDIEDYQYRIRCIDFDQQSYEGRKNLYLPQFFKENFAFVEMALAVLNKE
                     SIEQYQAEERTMITFRLATSRYRIKDLLDIMTADQISPPEKVEQLKKELGEYLHTNVY
                     EKCKSMGEIVKIHLKQTLRKNLLLVQKNLGKTKRKPL"
     gene            complement(3906..>4331)
                     /locus_tag="PLO70_16125"
     CDS             complement(3906..>4331)
                     /locus_tag="PLO70_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQZ76049.1"
                     /translation="NMVGKPVEGKELNTKIQFEFEQLKTIQNKIRTAYLIWKEPYMTV
                     GGDTFIHSMLAAAGLENIFANRIRYPEINVEELKAIHPELVLLSSEPYPFQQKHIDEL
                     QSALPQTKIILADGEMFSWYGSRLEKAPAYFSKLRNQLN"
BASE COUNT         1124 a          915 c          908 g         1384 t
ORIGIN      
        1 accgggaata gaggattctt cggtaggctg acatcctacg atctggatgg aagggttctt
       61 ttcttttaaa taccgtgaac atcccatgat ggtacccgtt gtacccattg aactcacgaa
      121 atgagttatg gattgattac tgtctttcca tatttccggc ccagtggttt tatagtgggc
      181 gatataatta tccgggttgg caaactggtt caggataaag gcttctccgc aggctgcttt
      241 ttcatcggca taatcacggc atacttcaat actttccagc aatgttactg tagcgccgaa
      301 agcttccatc gtcaacgttc tttctcttgt tgaggaagag ggcatcacta attcaatgtc
      361 caggtcatac aatcttgcga tcattgccag tgcaatccct gtgttgccac tggtagcttc
      421 aattaaccgg ttaccgggtt ttatatcacc tctttccaat gccgagcgga tcatattcaa
      481 tgccggccga tccttgacac tgccacccgg attattacct tccagtttgg catagatggt
      541 tacgttggga ttaggattta attttcttaa ttcaaccaaa ggagtatttc cgataaggtc
      601 tgcaatacca gccatgatga ttattttatt ttttccacaa cttcaatatc gggtcgatgg
      661 taaacaacag agtggggtgg taccgttttc gtaagccata catttccgcc gataatgcta
      721 tgatgaccta tgacagtttc accacccaaa atagtggcgc cggaataaat gatggcatgg
      781 tcttccacgg taggatgccg tttggtaaaa gccatcgact tgtcaacact tagtgcgcca
      841 agggtaacgc cctggtacaa ttttacatgg ttgccgatga tacaagtttc cccgatcact
      901 ataccagtgc catgatcaat gaaaaagtga ttaccaattt ttgcaccggg atgaatatcg
      961 atacctgttt ttgaatgggc ccattctgtc atgatgcggg gcagtaaggg aatatcaagc
     1021 aaatacaact ggtgggccac ccggtagaaa aagatagcat aaaagccggg gtaggcacgg
     1081 atgatctcaa atcttgtgtg ggcagcagga tcgccttcaa ggatcgctgt gatatcagta
     1141 tttagaacag cgtacagatc cgggataaca ttgaaaaatg cgtttgcctt ctccacatta
     1201 ttgcactggt cgcaggcttt ggtggcgttg aatatcctta tcagttctgc cttcatcttt
     1261 acaaaggcgg cttccacttc ttcggcagaa tccatgttgc attctgctct ctccgggaat
     1321 aaaagacaaa ataatttcaa tacacatcct gcaatctctt catttggagg aacagcttct
     1381 atggcctgtt gctgtgaata gatatgatca taaaatgcct ggttcataat agttgccttt
     1441 accggctttg tgaaattagc ataatttata acagcggatc atgagcgggg ttgtgtatgc
     1501 tgttaaaaat aaaagccctg cagatgcagg gctttgttat tatgatgcag ttagtattat
     1561 gcattccggt taatacaatt caggtcttca aacgccagtt caagacgttt gctgaacgat
     1621 tcttctcctt tgcgcagcca aacccttggg tcgtaatatt ttttattggg tttgtcatcc
     1681 ccttctggat tgcccaattg tccctgtaaa taggcttcat tctttttata attttccagt
     1741 ataccttccc agaaagccca ttgcaggtca gtgtcaatat tcattttgat agcgccatag
     1801 ccaatcgctt ctcttatctg gtgctgtggt gaaccactgc caccatggaa tacgaagtaa
     1861 acaggttttg gggcggtctt aaattctttt tcaatatgca cctggctgtt gttaagaatc
     1921 accggacgta attctacatt gcccggtttg taaacgccat gtacattgcc aaaagcagcg
     1981 gccactgtaa aaagttttcc tactttgctt aattcccggt aagcataggc cacgtcttcc
     2041 ggttgtgtat agagtttaga attttctaca tcgctattgt ccacaccatc ttcttcacca
     2101 ccggtaacac caagttctat ttcaatgctc atacccagcg gctgcattct tttaaagaat
     2161 tcaactgaag tatgaatatt ttcttcaata ggttcttcac tcaggtcaag catatgtgag
     2221 ctgaacaggg gttgaccttt ttctttatgg aattgttcgc cggcatcgat caggccactt
     2281 atccagggca accatttttt ggcagcatgg tctgtatgca gcacaactgg aacgccatag
     2341 tacctggcga cgttgtgaat atgcagggca ccggatatac caccggaaat atttccctgc
     2401 aggttatcat ttggcattcc tttcccggcg atgaactggg ctcctccgtt ggagaactgt
     2461 acaataacag gagagttgac ttttgcagca gtttctaagg tagcattgat agtgttagta
     2521 ccaatcgtgt tgacagcagg taaagcaaat tgattatccc tggcatcatt gtagagtgct
     2581 tccagttcgt caccgaacag cacaccggct ctgtactttt tcattaagtt ttaatttttt
     2641 taaaagtggc aaaaactttc agcaggcagg caatcgttaa aggcctgcta atatagaata
     2701 tttattgaat tgaaaatgcg aacaggtgtt agagtggttt gcgtttggtc ttacccaggt
     2761 ttttctgtac cagtaataaa tttttacgga gggtttgctt caaatgtatt tttacaattt
     2821 cccccatcga tttgcatttc tcatacacat tggtatgcag gtattctccc aattcttttt
     2881 ttaattgttc cactttttcg gggggcgata tctggtctgc ggtcattata tcgagcaggt
     2941 ctttgatacg ataacgggag gtggccaacc ggaaagtgat cattgttctt tcttctgcct
     3001 ggtattgttc aatggattct ttattaagaa cagcgagggc catttccacg aacgcaaaat
     3061 tctctttgaa aaattgcggc aggtaaagat tctttctgcc ttcataggat tgctggtcga
     3121 aatctataca gcggatccgg tactggtaat cctcaatatc aggagtaatt cctattacaa
     3181 agttgtagct ccgcatatca cccagcagcc gcacgaagca tctttcgttg aatttcacaa
     3241 attctttcgc taatcgtatt ttattggttt ccgggctatc catcatttgt ttgataaata
     3301 catcccccgg aatgccggga atatgttctt ctaccaatgt attatcacag gtaaaaaaag
     3361 tgatacggtt tggagaaagg atatgttcca gttccaggcc gtatatcctg gaggcatctg
     3421 cgatcttgat ataatagtga tcgtaattat cgttgaactt attgacaatc cggatacgga
     3481 aggggtttga atttccgaat gcacagtaat caattctggc cacatccagg tgacttataa
     3541 aactgaaatc accttctgtt ttcagtatgg cgtatatttt aaccagtccc tggcggatat
     3601 aatcccagtc acgactgtcg tacgaagttt tttcccaata agtatcattc ccgtgtttgt
     3661 ctttaatagg agtggaccag gtaaaattca acaggttatt ataggtaaca ggaagtttaa
     3721 cttcccggcc acggtttttc aggtagttac gcagcttgtc attaacagga tacattggtt
     3781 tcttacggga aggcttatca ccttcctttg tttcccggcc attctggtta tcaataaatc
     3841 tcatggggtg aataatcaat tgtgaatagt caataacgca tttctataaa ggttctaagg
     3901 tcgtattaat ttaattgatt acgaagtttg ctgaaatacg caggggcctt ttctaaccgg
     3961 cttccatacc aactgaacat ttctccgtct gccagtatga tttttgtttg gggtaatgct
     4021 gactgcaact catcaatatg tttttgttga aaagggtagg gttcagaaga aagtaaaacc
     4081 agttccgggt gtattgcttt tagttcttca acatttattt ccggataccg gattctattt
     4141 gcaaaaatat tctccaaacc tgctgctgcc agcatggaat gaatgaaagt gtcgccacca
     4201 actgtcatgt aaggttcctt ccagatcagg tatgcagttc ttattttgtt ttgaatcgtt
     4261 tttaattgct caaattcaaa ttgaattttt gtgttcagct cttttccttc aactggctta
     4321 cctaccatat t
//
LOCUS       DAORTI010000214         4317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_25900_length_4317_cov_8.719615, whole genome shotgun sequence.
ACCESSION   DAORTI010000214 DAORTI010000000
VERSION     DAORTI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25900_length_4317_cov_8.719615"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLO70_16130"
     CDS             complement(<1..449)
                     /locus_tag="PLO70_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQZ76050.1"
                     /translation="MCGIAGILQANISTYNKEQLAKMTGALAHRGPDGEGLWQNETGN
                     VLLGHRRLSIIDLSDSGQQPMHYQDRYTIIHNGEIYNYIELKEELQKKGYAFHSQTDT
                     EVITAAYDCWKDDCVNHFDGMFAFAIWDEKNRELFAARDRFGEKPFF"
     gene            complement(499..1137)
                     /locus_tag="PLO70_16135"
     CDS             complement(499..1137)
                     /locus_tag="PLO70_16135"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ76051.1"
                     /translation="MKIYLTILSFAFLLFSCNNKKESNATNTAKLNITDSATLARYER
                     PSVNPYAAVDVSPMDMSYYPADYPKLKMAGSISSPPLARVIYSRPHLQGRHIFHEVLK
                     YDEPWRLGANEATELDLYSDATIQNKKIKAGRYILYCIPHTDSWTIVINTNIDSWGLE
                     PDAKNDVARFEVPVIKTNNSLEYFTIVFEKKGTTAELLMAWDNAEVRLPMRF"
     gene            complement(1149..1709)
                     /locus_tag="PLO70_16140"
     CDS             complement(1149..1709)
                     /locus_tag="PLO70_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQZ76052.1"
                     /translation="MKKAFLLLMIFQLFLFSLLAQTLPPVDKSPMDMGYYPANYPVLK
                     IQDKLTEPLAARVVYSRPQKAGRTIFGGLVKYGEVWRIGANEATEIEFFKQVHIGGKK
                     VPKGRYTLFAIVNESSWTFIINKENDTWGSFRYDPKKDVVRTTVTVQKNDAVLESLAM
                     AFEKATGGFNLIVAWDNVKAVLPVTF"
     gene            complement(1763..2311)
                     /locus_tag="PLO70_16145"
     CDS             complement(1763..2311)
                     /locus_tag="PLO70_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HQZ76053.1"
                     /translation="MISDFVKGKKKFDYPSAIQKGIILHRFIDTFTDSHEATREAKEI
                     FRPAYRLYSGAFTDVVYDHFLAVDENEFPGNSLYDFSQAVYGTLNQNSQWFPEKFAGM
                     FPYIKEQNWLYHYRTRWGTGKSLGGVVRRSLHLTESETAFQLFEQHYQLLQDCYRHFW
                     AAVKPFAFKKFEELQNNRNTTT"
     gene            complement(2400..3068)
                     /locus_tag="PLO70_16150"
     CDS             complement(2400..3068)
                     /locus_tag="PLO70_16150"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HQZ76054.1"
                     /translation="MNKFLKILVWPIMMVPPVYLAMVWDKLPQQVALHFDLKGNADRF
                     GSKNELLTTVIILTVMNILVYLILTNIYRIDPKKYAAENKERLQRLGVGIVVFMSALL
                     CMILYSSSHGNISFNTGLVLSAVGLLFAFIGNYLPNLKPNYFAGLRLPWTLENADNWK
                     KTHALAGKLWFAGGLLLAVTCLFMPPVAAFILFTAVMIIITLIPCVYSYQLYKKQKNI
                     NPVN"
     gene            complement(3071..3334)
                     /locus_tag="PLO70_16155"
     CDS             complement(3071..3334)
                     /locus_tag="PLO70_16155"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HQZ76055.1"
                     /translation="MNIVFKALNDPTRREILELLQEKDMTAGEIVEQFNISGPSISHH
                     LDLLKQAKLVIAEKEGQFIYYSLNTTVVDELMKWFLQFKSKKK"
     gene            3613..4050
                     /locus_tag="PLO70_16160"
     CDS             3613..4050
                     /locus_tag="PLO70_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76056.1"
                     /translation="MKKMIMALAVLVSTLSAFAGEEKITPKVLNAFKSEFNTAKEVEW
                     TVGHNYYKAAFVYNDKHVFAYYNQDGELLGLTRYLSPDDLPLNLFSDLKKNYCKYWIT
                     DLFEVANTEGTTYYITLENADTKIVLKASAESEWSLHEKTRKA"
BASE COUNT         1253 a          878 c          863 g         1323 t
ORIGIN      
        1 taaaaaaatg gtttttctcc aaaacggtct cttgccgcaa ataattccct gttcttttca
       61 tcccagatgg caaaagcaaa catgccatca aaatgattta cacaatcatc cttccagcaa
      121 tcatacgctg ccgttattac ttcagtgtca gtttgggaat gaaaagcgta tccttttttt
      181 tgtaattctt cttttaattc gatgtagtta tagatctctc cgttgtgaat gatcgtgtag
      241 cggtcctggt agtgcatggg ttgctgacca ctgtcactta agtcgataat cgaaagacga
      301 cggtgcccca gtaagacatt gccggtttca ttttgccaca agccttctcc gtctggccca
      361 cggtgagcca gtgcacctgt catttttgcc agttgttctt tattgtaggt cgaaatattt
      421 gcttgtagta ttcccgcaat gccgcacatg ctgtaaatat ctaaaataat ttccggcagt
      481 gcagataaaa atccgggctt agaaacgcat gggcagtctt acttctgcgt tatcccatgc
      541 catcagcagt tcagcagtag tgcctttttt ctcaaatacg atggtaaagt attcaagtga
      601 attattggtt tttatcacag gtacctcaaa ccgggctaca tcgtttttgg catcgggttc
      661 aagaccccag ctatctatat tggtgtttat cacaatggtc cagctgtcag tatgcgggat
      721 acaatacaat atatagcggc cagcttttat ttttttattc tgaatagtgg catcgctgta
      781 cagatcaagc tctgtggctt cattggcacc cagtctccac ggttcatcat atttcagtac
      841 ttcatgaaaa atatgccggc cctgtaaatg gggacggctg tatatcactc ttgccagcgg
      901 tggagaactt atagagccgg ccatttttaa ctttggataa tctgccggat agtagctcat
      961 atccatcgga gatacatcaa cggctgcata gggatttacg gaaggtctct cgtacctggc
     1021 caatgttgcg ctatccgtaa tgtttagttt tgcagtattg gtagcattac tctctttctt
     1081 gttgttacag gaaaagagca ggaaagcaaa agatagaatg gttaggtata ttttcataaa
     1141 gcagtgaatt agaaagttac cggcaataca gcttttacat tgtcccatgc gactataagg
     1201 ttaaagcctc ctgttgcctt ttcaaatgcc atcgccagcg attccagtac cgcatcattt
     1261 ttttgaactg tcaccgttgt tcgtactaca tctttcttcg gatcatacct gaaggaaccc
     1321 caggtgtcat tttctttatt gataatgaag gtccagctgg actcattcac aatggcgaat
     1381 aaagtataac gtcctttcgg aaccttttta cctccgatat gcacctgttt gaaaaattca
     1441 atttcggtag cttcattggc acctatcctc catacctcac catacttcac caggcctcca
     1501 aagatggttc tgcctgcttt ttgcggccgg ctatacacaa ccctggctgc taagggctcc
     1561 gttagcttat cctggatttt gagcaccggg taattggcgg ggtaataacc catgtccatt
     1621 ggtgatttgt caactggcgg aagggtttgc gccaacaggg aaaacaggaa tagctgaaag
     1681 atcattaaaa gtaaaaaagc tttcttcata aaaattgact atttgcaaaa ataaaacgat
     1741 ttgtttgttc agaagttacc atttatgtag tggtattcct attgttctgc agctcttcaa
     1801 actttttgaa agcaaaaggt ttgacggcag cccagaaatg acggtaacaa tcctgtaaaa
     1861 gttggtagtg ctgctcaaat aattgaaaag cggtttcact ttctgttaag tgcagggaac
     1921 ggcggaccac accgccaaga cttttaccgg tgccccaccg ggtccggtaa tgataaagcc
     1981 agttctgttc ttttatataa ggaaacatac cggcaaattt ttccgggaac cattgtgaat
     2041 tttggttgag tgtgccataa acggcctgcg aaaaatcata caatgaattt cccgggaact
     2101 cattctcatc aaccgctaaa aaatgatcat acaccacatc tgtaaaggca ccactgtaca
     2161 aacggtacgc tggtctgaat atttctttcg cttcccgggt ggcttcatgg ctatctgtga
     2221 acgtatcaat gaaccggtgc aggataattc ctttttgtat ggcagatgga tagtcaaatt
     2281 tctttttgcc tttcacaaaa tcgctgatca tattacccac cagtatttca gggtggttaa
     2341 atgagagata tgcgtgggct aagtaattga ttagtcagat ttttagaaaa gaattaaaat
     2401 taattaaccg ggttgatgtt tttttgtttt ttataaagct ggtaggaata cacacaggga
     2461 ataagggtaa ttattatcat tactgctgta aacaatataa aagctgctac cgggggcata
     2521 aacaggcagg taacggccag taataatcca ccggcaaacc agagcttacc ggccagggca
     2581 tgtgtttttt tccagttgtc tgcattttcc agtgtccagg gtaaacgcag gcctgcaaaa
     2641 taattaggct tcaggttggg taaataattt ccgataaagg caaatagtaa acctaccgca
     2701 gaaagcacca ggccggtatt gaaactgata tttccgtgtg aactgctgta aaggatcatg
     2761 cagagcaatg cagacatgaa tacaactata ccaactccca gccgttgcaa tctttcttta
     2821 ttctccgctg catatttctt gggatctata cgataaatat tggtaagaat aaggtagacc
     2881 aggatattca ttacggttag gataataaca gttgtcagca gctcattttt actgccaaac
     2941 ctgtccgcat ttcctttcag gtcaaaatga agtgccacct gttgcggcag tttgtcccat
     3001 accattgcca ggtaaacagg cggtaccatc atgattggcc agaccaggat ctttaaaaat
     3061 ttattcataa ctatttcttt tttgatttga attgtaaaaa ccatttcatt agttcatcta
     3121 ctactgtagt gtttaatgaa taatagataa attgtccctc tttctccgca atgacaagtt
     3181 tagcctgttt aagcaggtca agatgatgtg aaatacttgg cccggatatg ttgaattgct
     3241 ccactatttc acctgctgtc atgtcctttt cctgcaataa ttccagtatc tcccggcggg
     3301 taggatcatt cagggcttta aacacaatat tcatgtctga attttataat tatatattta
     3361 gacaaatatc taaataatat tttaaccgga aaaataattt tactggcatc acataaacat
     3421 gtggtgaata ctgatggccg gtatttaaaa atggccagcg gttaatattt accaacttct
     3481 gttaacagca gttaacacag gcttccgtta atcacaattg acttgtaatg gggtgtaacc
     3541 taatctacat ttgtaccgtc acactataca gaacgatcaa ttattaacct taaccttttt
     3601 aaaaaatata gtatgaaaaa gatgataatg gcactggctg tacttgtaag tacattaagt
     3661 gcatttgccg gagaagaaaa aattacgcca aaagttttaa atgctttcaa aagcgaattc
     3721 aatactgcca aagaagtaga gtggactgtt ggccacaatt attataaagc ggcctttgtg
     3781 tataatgata agcatgtatt tgcctattat aaccaggatg gtgaattgct gggtcttacc
     3841 cgctatctca gcccggatga cctgccactg aacctgttca gcgatctaaa gaaaaattat
     3901 tgcaagtact ggatcactga tttgtttgag gtagcaaaca cagagggtac tacttactat
     3961 atcaccctgg agaatgctga tacaaagatt gttttgaaag catcagcaga gagtgagtgg
     4021 agtttgcatg aaaaaacaag aaaagcataa atatagtacc ggaagttagt tttggtgtgt
     4081 ggtaaaacaa gccctggtgc gccaatggca gccggggttt tttatgcagc gtatacagca
     4141 gttaataaat attttctatc gttaatgtat ttaacaatga ctgccgtacc tcacaattca
     4201 cttgacttcg ttttatcacc atcgtatttt tgaatcgtac aatcaacaat cgttaatcat
     4261 taaacctaaa gccatgaaaa aaattatctt atcactgtca ttactactga cagtcgc
//
LOCUS       DAORTI010000215         4302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_26043_length_4302_cov_6.149046, whole genome shotgun sequence.
ACCESSION   DAORTI010000215 DAORTI010000000
VERSION     DAORTI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26043_length_4302_cov_6.149046"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..714)
                     /locus_tag="PLO70_16165"
     CDS             complement(115..714)
                     /locus_tag="PLO70_16165"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQZ76057.1"
                     /translation="MENTFNALINSFIDTKVGISENFLPLSLATQLKDILLTLYAKDQ
                     LLSAGTGNNKMVTHNKSVRSDMIYWLDRNHKNQYENEFFDLIDNFVSYLNSTCYTGIT
                     GYEFHYALYEKGSFYKKHLDQFRNNNSRKYSMIMYLNEDWQKADGGELCIYHTERRQN
                     IAPENGKMVFFKSSELEHEVLLTNKARMSITGWLKTSTQ"
     gene            877..1686
                     /locus_tag="PLO70_16170"
     CDS             877..1686
                     /locus_tag="PLO70_16170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQZ76058.1"
                     /translation="MSIIYRKKFLASLLFLLWLIPSCDTVKKKKVLEDFKYESFRDSL
                     NRDSTGITADTTNIFDASVFIPGIDSLDTLLKRIDTLWHQDIALMLRIDSLKSKGKNE
                     KIVTSDEMTTARSNINALDSFKNIRNIIVPVSCKEKECFLYAEINKSAQRLDLYIDGE
                     WKDSFRVSTGKGKYETPNLNVRPSGPLFIKYTSRKFPGGNYQGLGNMPYAVFIRGGYA
                     IHGTTPGNFSKLGTRASHGCIRLHPDNAKIFYELVKKVGLDNAWVTIRDSL"
     gene            1714..2265
                     /locus_tag="PLO70_16175"
     CDS             1714..2265
                     /locus_tag="PLO70_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ76059.1"
                     /translation="MLQFSDPYIKLATTADRFAIINLLNKAYRGESSKQGWTTEANLI
                     AGDQRTDEANLLQVMNQPGSVFLKYSSDEQLITGCVNLQQHGDRIYLGMFSVSPQEQG
                     RGIGKHMLKAAEEYARHLHCNAIYMSVISVRHELIKWYQRHGYCDTGERKPFIEDGLT
                     GKHLQPLEFMILEKSLSPGTSTK"
     gene            2378..2920
                     /locus_tag="PLO70_16180"
     CDS             2378..2920
                     /locus_tag="PLO70_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76060.1"
                     /translation="MKAEKIVQFVSFETTLDSEQFIAEWEQYTRPLNSDLDVTMQQSE
                     KNGVFRYLVQHRCASGDFQFVVTKGKRSSRIPEVGIKEKQIGGYSVLQAERKGDTQTG
                     ESKVFVFLIQPQADLDSYRQLPRHGKLNIYEAFYENCQYAYILEFFLKDEHISAFQEE
                     LKLNHADMILICKECNLQES"
     gene            complement(3047..3754)
                     /locus_tag="PLO70_16185"
     CDS             complement(3047..3754)
                     /locus_tag="PLO70_16185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQZ76061.1"
                     /translation="MTTFLENNVSGIPRIGDTAPSFTAVTTQGTIHFPQDYKGKWVIL
                     FSHPADFTPVCTSEFMTFAKQEAEFARLNTQLVGLSVDGLYSHIAWLRTIKEKIEFRD
                     MKNMEIKFPLIEDITMDVAKKYGMIQPNESSTKAVRAVFFIDPKGIIRAMIYYPLSLG
                     RNFNELKRVIIGLQTADKHAVAIPADWQPGEDVIVPPANSCGVAADRMAGKEDNVYCK
                     DWFFCMKPLPKEELELA"
     gene            4005..4289
                     /locus_tag="PLO70_16190"
     CDS             4005..4289
                     /locus_tag="PLO70_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQZ76062.1"
                     /translation="MSGTVNESGLTPEQVVNSRSKHGSNTLEIQEDRVFGHVLKEVVL
                     EPMFILLLVACIIYFIAGQYQEGIIMLVSIFIVAGISLSRNTGAVMQYKL"
BASE COUNT         1282 a          785 c          861 g         1374 t
ORIGIN      
        1 gtcgttctta taatgaaaaa ttatggtgac tatcatcatt gataattatg attttagttt
       61 tattaaccgg gtaatgattt accagaccgg gtcacttaat ttattttgaa aagattattg
      121 ggtgcttgtt tttaaccacc ctgttatact cattctggcc ttattggtaa gtaaaacctc
      181 atgttccagt tcactgcttt taaagaagac cattttgcca ttttccggag ctatattttg
      241 ccgcctttca gtatggtaaa tacataattc tccaccatca gctttttgcc agtcctcatt
      301 taaatacatg atcatcgaat attttctgct gttattgttc ctgaactggt cgaggtgttt
      361 tttgtagaaa cttccttttt cgtacaaagc ataatggaat tcatatcctg tgataccagt
      421 atagcaggtg ctgtttaaat agcttacaaa attatctatc aggtcaaaaa actcattctc
      481 gtattggttc ttatgattcc ggtcaagcca gtaaatcatg tcacttctga ctgatttgtt
      541 atgcgttacc attttattgt tgccagttcc ggcagacagg agttgatctt ttgcgtaaag
      601 agtgagaagg atgtctttca gttgtgtggc aagagaaagg ggtaagaaat tttcagatat
      661 tcctacttta gtatcaataa agctattgat gagagcatta aaagtatttt ccaatattaa
      721 atgattgaac gctgtattgg gcagactgcc gttatgctgg tgaagatacc cttatttact
      781 ccccggtact cacttactta atttgcgtat ccatgctatc agaagaagta acaccatcag
      841 aagtagtaac tttgtgaaag aattcaatct atcagcatga gtattatata tcgtaaaaag
      901 tttttggctt ccttattatt tcttttatgg ttgattcctt cttgtgatac tgtcaaaaag
      961 aaaaaagtac tggaagattt taaatatgag agcttcagag attctctaaa ccgggatagt
     1021 actggtatta ctgctgatac caccaatata ttcgatgcat ctgtttttat ccctggtatt
     1081 gattcattgg atacattact gaaacggatt gatactttat ggcatcaaga cattgctttg
     1141 atgttgcgga tagattcatt gaagtcaaag ggaaaaaatg aaaagattgt tacttcggac
     1201 gaaatgacca ctgcccggtc caacattaat gcacttgact cctttaaaaa catcagaaat
     1261 atcattgttc ctgtttcctg taaggagaaa gaatgttttc tgtatgctga aatcaataaa
     1321 tcagctcaac gactcgattt atatattgat ggcgaatgga aagatagttt cagggtatct
     1381 accgggaaag gaaagtatga aacacccaac ttaaatgtaa ggccatccgg cccgcttttt
     1441 ataaaatata cttcccggaa atttcctggc gggaactacc agggattagg caatatgcct
     1501 tatgcagttt ttattcgggg aggatatgct atacatggca ctactcccgg caattttagc
     1561 aaattaggca ccagggcttc acatggatgt attcgtcttc atcctgataa tgcaaaaata
     1621 ttttatgaac ttgtaaaaaa agtaggcctt gacaatgcct gggtgactat cagggattct
     1681 ttgtaatttt ttaaaactac actttagtgt tctatgttac aattttctga tccatatata
     1741 aaacttgcaa ctactgcaga caggtttgct attattaatt tactcaacaa ggcctaccgt
     1801 ggtgaaagtt ccaaacaagg ctggaccaca gaagcaaatc ttattgccgg tgaccagcga
     1861 actgatgagg ctaacctgtt gcaggtaatg aatcagcccg ggagtgtttt tttaaaatat
     1921 agtagcgatg aacagctgat aaccggatgt gtaaacctgc agcaacacgg tgatagaatt
     1981 tacctgggta tgttcagcgt ttcaccgcaa gaacaaggca ggggtattgg taagcatatg
     2041 ctgaaggcgg ctgaagaata tgcccggcat ttacactgca atgccattta tatgtcagtc
     2101 atttctgttc gtcacgaatt gatcaaatgg taccaacgtc atggctattg tgataccggt
     2161 gaacgaaaac cttttattga agatggttta acaggtaagc acctgcagcc attggaattt
     2221 atgatattgg aaaaatcatt atcaccgggt acaagcacaa aatagctgac tttatttttt
     2281 ctggtacttc ggataatcaa gcgtttgtca ttcttttaac caatattgta aaacagataa
     2341 atacgatgaa accacttttt aagcttacct tgtaaaaatg aaagcagaga aaatagtgca
     2401 gtttgtttct tttgaaacaa ctttggatag cgaacagttt attgctgaat gggaacaata
     2461 tacaagaccc ctcaacagtg acctggatgt gactatgcag cagtctgaaa agaatggtgt
     2521 attcaggtat cttgtacaac atcgttgtgc ttccggtgat tttcagtttg tagtaaccaa
     2581 agggaaaagg tcatccagaa ttcctgaagt aggtataaaa gaaaagcaga taggggggta
     2641 ttctgttttg caggctgaga gaaagggtga tacacaaaca ggtgaatcca aggtttttgt
     2701 ctttcttatt caaccacagg cagacctaga tagttacagg caattacccc ggcacggtaa
     2761 attaaatatt tacgaagctt tttatgaaaa ttgccaatat gcttatattc tcgagttttt
     2821 tttaaaagat gaacacatat cagcatttca ggaggaattg aaattgaatc atgccgatat
     2881 gattctgata tgcaaagaat gtaatctgca ggaatcctaa aagtttgcat tgcagcactt
     2941 aattgcttac caattaatca ttgtttacta actgaatata ataatctgct cttgccttat
     3001 caaagtacaa gagcaggttt atggttatta tcaatcacta tcctgtctat gccagttcga
     3061 gctcttcttt gggtaatggt ttcatacaga agaaccagtc tttacaatat acattatcct
     3121 ctttacccgc catccggtct gccgccacac cacaactatt ggccggtgga acaataacgt
     3181 cttctccggg ttgccaatcg gctggaatag ccacggcatg tttatctgcg gtttgtaatc
     3241 caataattac ccgtttcaac tcattgaagt tcctgccaag ggacagtgga taatagatca
     3301 ttgccctgat tataccttta gggtcgataa agaaaacagc tcttactgct ttggttgaac
     3361 tttcatttgg ctggatcatg ccatattttt tagcgacatc catggtaata tcttcaatca
     3421 atggaaattt tatttccatg ttcttcatat ccctgaattc gattttctcc ttgatggtgc
     3481 gtaaccaggc aatatggctg tatagcccgt caacactcaa tcctaccaat tgcgtattta
     3541 accgggcaaa ttcagcttcc tgttttgcga aagtcatgaa ctcagatgta catacagggg
     3601 taaaatcagc cgggtgactg aataagatta cccatttgcc tttgtagtcc tgtggaaaat
     3661 gtattgtgcc ctgggtagtt acagctgtaa aggagggagc tgtatccccg atccttggaa
     3721 taccactgac attattttcc agaaaagttg tcatatgatt tgattttaac gttaaaaatt
     3781 cattttgggc aatctactgt tagtctgttg taacttccaa tgatagctgt cattcaatcg
     3841 ggtgattttg gtataatcgg ggctagaaaa tccaattatg ctaggggaat aaatgttgcc
     3901 ttctcccatt aatcttcctg aaaaatgaaa gaatggaata cctttcaact ttgcacatag
     3961 cctcagggag gattgttcat ttttaaaaat ctaaattgct tcacatgagt ggtacggtta
     4021 atgaaagcgg gttaactccc gaacaggtgg tgaacagcag gagcaagcat ggcagcaata
     4081 cacttgaaat acaggaagac cgtgtctttg gccatgtatt gaaagaagta gtgctggaac
     4141 cgatgtttat tttattgctg gttgcctgta tcatctattt tattgcgggg cagtaccagg
     4201 aagggattat aatgctggta tcgattttca ttgtggcggg tatctccctt tccaggaata
     4261 ccggagccgt aatgcaatac aagctttaaa aaaattatca gc
//
LOCUS       DAORTI010000216         4184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_27217_length_4184_cov_7.380722, whole genome shotgun sequence.
ACCESSION   DAORTI010000216 DAORTI010000000
VERSION     DAORTI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27217_length_4184_cov_7.380722"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3901)
                     /locus_tag="PLO70_16195"
     CDS             complement(<1..3901)
                     /locus_tag="PLO70_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQZ76063.1"
                     /translation="MKVTPALKKELADWLDTYWTTYLKGDIATWATFIRDDYRNIGGT
                     KEEILNSKQEIIDYTNSILNQLVGAVDIRNRKIELIPYGEYMMVNEFTDLYVKIESEW
                     TFYGPFRMSSLLEKTETGWIALHQHGSYPDMKAMEGEAFSIDALKAENAKLQAAVKQR
                     TIELEQNNRKLEIEAATERVRVQAMAMQQPADLGKVNKELLHQLTTLEIPGLAGVSFY
                     FVDPDEIVTVWDLSSPGNLNDPHSYSFRFDSKKYPVLGEFVGILKSTQQDYFILDFPK
                     DKLLQAIEELKDIDIKIYNNFKHAVESGALLHQWNPAARITDGILSIDLMIPPTDDTK
                     TIVLKMAGAFNQAYQRFLDLQKAEAQARESQIESALERVRTASMAMRDSSALGEIIYK
                     LYGELTKLDAKLDRCFIMVVNPENKGITWWMAGQEGLLAENGFFVQMNQHPSHLMYLD
                     NCKKRKKKWTYLFEGKEKRDWDRYGFSETELARLPEPVKAFMAAAEKVHLSGSSDQFG
                     SLVTGSFEPLPEEQQEIISRFTIAFNQAYIRFLDLQKAEAQAREAEIELALERVRART
                     MAMQQSTELPDAATILFQQVQSLGMPAWSAGYCIWNEDKSAVTLWMSSEGVLQPPFKA
                     PTTDDELFIEMRKGQEDGNSFHVVEMGGKKLEKHYQYMRTLPVVGEILDSIIAAGHPL
                     PTFQIMHHAYFSKGFLLFITYEPVPAAHDIFKRFGKVFDQTYTRFLDLQKAEAQAREA
                     QIEAALERVRSRSMAMHKSEELADLSLELVKQVQALGVATWFCAFNIYDEDPEGSLEW
                     GSNGQGTFPKYRTPREGIFLRYYEAGQKGESLLINEIGEDECPAHYEYLCTLPGVGEQ
                     LLKMKGDGIPFPASQIDHVAFFKYGYIIFITYDPCPGAYDLFKRFAKVFEQTYTRFLD
                     LKRAEAQAKEAQIEAALERVRAKAMAMHSSEDLALTVDAFFVELRSLRVTPRRCGVTL
                     IDEETRVADLTVTTATGDDKSKKIIAKLKLAGHPVLDGVYDHWKQQKEFHPVLQGAEI
                     KEYYQVMNQQVSFPDFASDEIQYGHYFFFEEGGVFAWTDMKLSEVELQIFRRFTSVLS
                     LTYKRYKDLQQAEKLAQQAEEDLVKLKEEKKRTEDALAELQVTQKQLIQSEKMASLGE
                     LTAGIAHEIQNPLNFVNNFSEVNKELLEELKAERLKPNAERDENLESELIHDIITNEE
                     KINHHGKRADAIVKGMLQHSRSSSGQKEATDINALADEYLRLSYHGLRAKDKSFNAIL
                     KTDFDSSIVNINIIPQDIGRVILNLITNAFYVVNERQK"
     gene            complement(3898..>4184)
                     /locus_tag="PLO70_16200"
     CDS             complement(3898..>4184)
                     /locus_tag="PLO70_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQZ76064.1"
                     /translation="AGQPYEPTVEVSTKKVKDKVEIKVKDNGNGIPQKILDKIFQPFF
                     TTKPTGQGTGLGLSLSYDIVKAHGGELKVETNEGKGSEFIIQLPINMNNG"
BASE COUNT         1062 a          975 c          783 g         1364 t
ORIGIN      
        1 ccttctgcct ttcattcact acataaaatg cattggtaat taaattcaaa atcactcttc
       61 ctatatcctg cgggatgata ttgatattga cgatactgct gtcaaagtct gtttttaaaa
      121 tggcgttgaa agatttgtct tttgcccgaa ggccatgata ggagagccgc aaatattcat
      181 cggctaaggc attaatatct gtcgcttctt tctgcccgct gctgctgcgg ctgtgttgca
      241 gcatgccttt tacaatggcg tcggcccgtt tgccgtggtg atttattttc tcttcgttag
      301 taattatatc gtgtattaat tcactttcta aattttcatc tctttctgca ttcggcttta
      361 agcgttccgc tttaagctct tctaataatt ccttatttac ttccgaaaaa ttattaacga
      421 agttcaacgg gttttgaatt tcatgggcaa taccggcggt gagttctccc aggctggcca
      481 tcttctcgga ttgaatcagt tgtttttgcg taacctgcaa ttcagccagt gcatcttctg
      541 ttcttttttt ctcttctttt agtttcacca ggtcctcttc agcttgctgg gccaattttt
      601 ctgcctgttg aagatctttg taacgtttat aagtaaggct taacaccgaa gtaaatctcc
      661 ggaatatctg caactcaact tctgataatt tcatatcagt ccaggcaaat acacctcctt
      721 cttcaaagaa aaaataatgt ccgtattgaa tttcgtccga agcaaaatca ggaaatgaaa
      781 cttgctggtt cattacctgg tagtactctt ttatttcagc accctgtaaa accggatgaa
      841 attctttttg ttgcttccaa tgatcataaa cgccatccaa tacaggatgg cctgccagtt
      901 tcaatttcgc tattatcttt ttagatttat cgtctcccgt ggctgtggta acagtaaggt
      961 ctgccaccct ggtttcttca tcaattaaag tgactccaca cctccgggga gtaacccgaa
     1021 gagaccgcaa ttcgacaaag aatgcatcaa cggttaaggc cagatcctcc gaactatgca
     1081 tggccattgc ttttgctctc actctttcta aggcagcttc aatctgtgct tcttttgcct
     1141 gcgcttctgc tcttttcaga tcaaggaaac gggtataggt ttgctcgaat actttggcaa
     1201 aacgtttaaa aaggtcgtac gccccggggc agggatcgta ggtaataaaa atgatatagc
     1261 catatttaaa aaacgcaaca tggtcaattt gtgaagcagg aaaggggatt ccatcgcctt
     1321 tcattttcag caactgttct cctacaccag gcaaagtgca aaggtattcg taatgagcag
     1381 gacattcatc ttcgccgatt tcattgatca ataatgattc tcctttttgc cccgcttcat
     1441 aatagcgaag aaatatacct tctctcggcg ttctgtactt tggaaatgtg ccctggccat
     1501 tactgcccca ttccaatgat ccttcaggat cttcatcata aatattaaat gcacagaacc
     1561 aggttgcaac acctaaagcc tgaacctgtt ttaccaattc aagagaaaga tcagccagtt
     1621 cttcactttt atgcatggcc atggagcggc tacgtactct ttccaaagcc gcttctatct
     1681 gtgcctccct tgcctgtgct tctgcttttt gcaggtcaag aaaccgggta taggtctggt
     1741 cgaatacttt accaaacctt ttgaagatat catgtgcagc aggaacaggt tcataagtaa
     1801 tgaacaacag aaaccctttt gaaaaatagg catggtgcat aatctggaaa gtgggcaatg
     1861 gatggccggc tgcaataatc gaatccagta tttcacccac tacaggaagt gtacgcatat
     1921 actggtaatg cttctctaat tttttgccac ccatttcaac tacatgaaat gaattgccat
     1981 cttcctgtcc ctttcgcatt tcaataaata attcatcatc tgttgtggga gctttaaatg
     2041 gtggttgcaa aaccccttca ctgctcatcc ataatgtaac ggctgattta tcttcattcc
     2101 atatacaata gccggcactc caggctggca tgcctaaaga ttgcacttgt tgaaataata
     2161 tagtagcagc atcgggcagt tctgtacttt gctgcatggc catagttctt gcccttactc
     2221 tttctaacgc taattctatc tctgcttctc tggcctgtgc ttctgctttt tgcagatcaa
     2281 ggaaacggat atatgcctgg ttgaaggcga tcgtaaaacg actgatgatt tcctgctgct
     2341 cttccggtaa aggttcaaaa ctgccggtta ccaatgaacc aaattgatca gaagaaccgg
     2401 acagatgtac tttctctgct gcggccataa acgctttgac gggctccggt aacctggcca
     2461 gttctgtttc actgaaacca tagcggtccc agtctctctt ttcctttcct tcaaacaggt
     2521 atgtccactt tttcttccgc ttcttacaat tatcgagata catcaaatga gaagggtgct
     2581 gattcatttg aacgaagaag ccattctctg ccaacaggcc ttcttgcccg gccatccacc
     2641 aggtgatgcc tttgttctca ggatttacca ccataataaa acatcggtcg agtttggcat
     2701 ccagttttgt gagttctcca tacagtttgt aaataatttc gcctagcgct gatgaatccc
     2761 gcatagccat agaagcggtt ctcactcttt ccaatgccga ttcaatttgt gattctcttg
     2821 cctgtgcttc ggctttttgt aaatcaagga agcgttgata agcctggttg aatgccccgg
     2881 ccattttcaa tacaatagtt tttgtatcgt cagtaggtgg aatcatcagg tcaatggata
     2941 aaataccgtc tgtaattctt gcagccggat tccattgatg tagtaaggca ccgctttcta
     3001 ctgcatgttt aaaattattg tagattttaa tgtcaatatc ctttaactct tcgatagcct
     3061 gcagcaattt atcttttgga aaatccagaa tgaaataatc ctgctgcgtt gattttaaaa
     3121 ttcctacaaa ctcacccagg acagggtatt tttttgaatc aaaccgaaag gaataactat
     3181 ggggatcatt cagatttccg ggacttgaca ggtcccagac tgtaactatt tcatcgggat
     3241 caacaaagta gaaactaact ccggctaatc cggggatctc cagtgtagtt agttgatgaa
     3301 gcaattcttt atttactttt cccagatcag cgggctgctg catagccata gcttgcaccc
     3361 taacccgttc tgttgccgcc tcaatttcca gtttacggtt attctgttcc agctcaatag
     3421 tacgttgttt aaccgctgcc tgcagcttcg cattttctgc ttttaaagca tcaatggaaa
     3481 aagcttctcc ttccattgct ttcatgtcgg gataggaccc atgctgatgt aaagcgatcc
     3541 atccggtctc tgttttttcc agtaatgatg acatccggaa aggaccataa aatgtccatt
     3601 cgctttcaat ttttacgtac agatctgtaa attcattcac catcatgtac tcgccataag
     3661 ggatcagctc tatcttccgg ttacgtatgt caactgctcc aaccaattga ttcaaaatac
     3721 tattggtgta atcgatgatt tcctgcttgc tgttcaaaat ctcttccttt gtgccaccta
     3781 tattccggta atcgtcccga ataaaagttg cccatgttgc aatatcacct tttaaataag
     3841 tggtccaata ggtatcaagc caatcagcta attctttttt tagcgctgga gttactttca
     3901 tccgttgttc atattaattg gtaattgaat aataaactca cttcctttcc cttcatttgt
     3961 ttccactttt aactccccac catgcgcctt cacaatatca tagctcaaac tcaatcctaa
     4021 tccagttccc tgcccggtgg gtttggtggt aaagaagggt tgaaagattt tatctaatat
     4081 tttttgaggg atcccgttgc cgttgtcttt taccttaatc tcaactttat ccttgacttt
     4141 cttcgtactt acttcaacgg ttggttcgta cggctgtcct gctg
//
LOCUS       DAORTI010000217         4153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_27533_length_4153_cov_6.669351, whole genome shotgun sequence.
ACCESSION   DAORTI010000217 DAORTI010000000
VERSION     DAORTI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27533_length_4153_cov_6.669351"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLO70_16205"
     CDS             complement(<1..288)
                     /locus_tag="PLO70_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HQZ76065.1"
                     /translation="MTKVVIIGPGHPLRGGLATFNQRLAKEFNDQGFDCSIYSFSLQY
                     PSFLFPGKTQYSDDPAPEGIIIHSAINSVNPFNWISVCNKLKKERPDLIIVR"
     gene            complement(288..1229)
                     /locus_tag="PLO70_16210"
     CDS             complement(288..1229)
                     /locus_tag="PLO70_16210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQZ76066.1"
                     /translation="MDLSIVIPLIDEAESLPELTAWIEQVMKENHYSYEVIFVDDGSS
                     DNSWEVIENLRAKNACIKGIKFQRNYGKSAGLNEAFRAASGDVIITMDADLQDSPDEI
                     PELRRMIIEDGYDMVSGWKKKRYDNTLTKNIPSKFFNAVTRSMSGIKLHDFNCGLKAY
                     KNKVVKGIEVYGEMHRYIPVLAKWAGFKKIGEKVVEHRARKYGVSKFGWRRFVNGFLD
                     LLSITFVGKFSKRPMHFFGLWGSLFFLVGLGISIYLIVSKFIDSSFALTNRPGFYLAL
                     TSLIIGMQLFLAGFIGELISRNSSGRNTYLVETKTGL"
     gene            complement(1232..1723)
                     /locus_tag="PLO70_16215"
     CDS             complement(1232..1723)
                     /locus_tag="PLO70_16215"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQZ76067.1"
                     /translation="MQLRLSPAIKGLITAVLMIAVALVIDAKKEAADPRLQYLIFIVY
                     GAGIIWTLLSFRQSAAFNGKFAELFSQGFKCFIVVTLIMVAFTAVFIQLHPEFALQEA
                     AYQREQLVKLNDKTPTEIEDLVARVKKQYPVRYISASIFGYLILGAAVTAVASVLLTR
                     RRY"
     gene            complement(1734..2996)
                     /locus_tag="PLO70_16220"
     CDS             complement(1734..2996)
                     /locus_tag="PLO70_16220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQZ76068.1"
                     /translation="MKLLIKQARIVDPSSPFNGQSADILIDNGIISKIGIALPDKADK
                     EIAIEGLHASPGWVDVFANFADPGYEFKETLETGAAAAAAGGYTDVMVIPNTNPSIHN
                     KAGVEYITHKSKSLPVNIHPIGAITKNTEGKELAEMYDMKSSGAAAFSDGINCVQSAG
                     LLVKALQYVKAFDGILIQLPDDKSINPHGLMNEGIVSTQLGLPGKPAMAEELIVARDI
                     KLARYAGSRLHFTGVSTKKSLEYIKRGKESGTAVSCSVTPYHLFFCDEDLTDYNTNLK
                     VNPPLRSKEDRAALQQAILNGTVDSLATHHLPHEYDSKVIEFEYAKFGMIGLETAYGV
                     LATAVPGITAARCVELLAVNPRKLFGLNQASIKEGNPATLTLFSPAVKWTVSPENIKS
                     KSSNSPFIGRELTGKVAGIINGDKIVIS"
     gene            complement(3071..3144)
                     /locus_tag="PLO70_16225"
     tRNA            complement(3071..3144)
                     /locus_tag="PLO70_16225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3108..3110),aa:Pro,seq:cgg)
     gene            complement(3209..3282)
                     /locus_tag="PLO70_16230"
     tRNA            complement(3209..3282)
                     /locus_tag="PLO70_16230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3246..3248),aa:Pro,seq:ggg)
     gene            3478..3777
                     /locus_tag="PLO70_16235"
     CDS             3478..3777
                     /locus_tag="PLO70_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76069.1"
                     /translation="MDRKSNVKDIISWLFGIVVVAIGLVNLFWGNDPGFGVFLLLLSL
                     VYFLPVNAILRKLTGFTIPKLGIVKIVLGIFILWASLGVGELFDKIELMMNSFRE"
BASE COUNT         1165 a          884 c          843 g         1261 t
ORIGIN      
        1 gcgaacaatg atcagatcag gtctttcttt tttaagttta ttacaaacac tgatccagtt
       61 gaatggatta acagagttta ttgctgaatg tataataata ccttcagggg ccgggtcatc
      121 actgtattgg gttttacccg gaaacaagaa ggagggatat tgtaatgaaa aggaataaat
      181 agaacaatca aacccctggt cattgaattc ttttgccagc cgctggttga atgtggccaa
      241 tccaccccgc aacgggtggc cgggtcctat gatgaccact tttgtcatta caacccggtt
      301 ttggtttcaa caagataagt attgcggccg gaagagttcc tggagatcaa ttcgccaatg
      361 aaacccgcaa gaaataactg catacctata atgagcgaag taagtgctaa ataaaacccg
      421 ggcctgttgg tgagtgcaaa actgctgtct ataaactttg atacaataag atatatggaa
      481 atgcccagtc caaccaggaa gaataaagaa ccccataatc caaaaaaatg catcggtctc
      541 ttggaaaatt ttccaacgaa tgtgatagaa agtaaatcga gaaaaccgtt tacaaatctt
      601 ctccagccaa attttgacac gccgtatttc ctggccctgt gctctactac tttttcgcca
      661 atttttttga acccggccca tttagccagt accggaatgt agcggtgcat ttcaccgtat
      721 acttcaatgc ctttcactac tttattttta tacgctttca acccgcaatt aaagtcgtgt
      781 agttttatgc ctgacatgct gcgggtaaca gcgttgaaaa acttggaagg gatatttttt
      841 gtgagtgtat tgtcgtaacg ttttttcttc catccactca ccatatcgta gccatcttct
      901 atgatcattc ttctcagctc aggaatttca tccgggctat cctgcaggtc agcatccatt
      961 gtaatgatca catcaccact ggctgcccgg aaggcttcat ttaaaccggc agatttacca
     1021 taattacgtt gaaacttgat ccctttgata caggcatttt tggctctgag gttttcaatg
     1081 acttcccatg aattgtcact gctgccgtca tcaacaaaaa tgacctcata actgtaatga
     1141 ttctccttca ttacttgttc tatccatgct gttaattccg gcagtgattc tgcttcatct
     1201 ataagtggta tgacgatgga taggtccatg gttagtatct tcttcttgtt aatagaaccg
     1261 aagcgactgc tgtaacagct gcgccaagta tcaaatatcc gaaaattgaa gcggatatat
     1321 atcttaccgg gtattgtttt ttcacccttg caacaaggtc ttcaatttcg gttggtgttt
     1381 tatcgttcag cttaaccaat tgctcccgtt gataagcggc ttcctgcagg gcaaactccg
     1441 ggtgcagttg tatgaacact gccgtaaatg ccaccataat aagagtgaca acaataaaac
     1501 atttaaatcc ctggctaaaa agttcagcaa atttcccgtt gaaggcagca gattgcctga
     1561 aactaagtaa ggtccagatg atacctgctc cataaacgat aaaaatcaag tattgtaatc
     1621 ggggatcagc tgcttctttt tttgcgtcaa taaccagtgc aaccgctatc attaatacag
     1681 cagtaattaa tcctttgata gcaggtgaaa gtcttaactg catgaataat gatttagcta
     1741 atgacaattt tatcaccgtt gatgattcca gccactttac cggtcagttc cctgccgata
     1801 aaaggagagt tggatgattt tgatttaata ttttccgggg atacagtcca ttttactgcc
     1861 ggactgaaca aagtaagtgt tgcaggattt ccttctttaa tggaggcctg gtttaagccg
     1921 aataatttcc tcggattaac agccagcagc tcaacacatc ttgctgcagt tattcccggt
     1981 acagcagtag caagcactcc ataggctgtt tccaaaccaa tcataccaaa ttttgcatat
     2041 tcaaactcta tcactttgct gtcgtattca tggggcagat gatgagttgc caggctatca
     2101 actgttccat ttagtattgc ctgctgcaaa gccgccctgt cctctttgct gcgtagtgga
     2161 ggatttactt ttaaattggt attataatct gtcaaatctt catcacagaa aaaaagatga
     2221 taaggtgtaa cggaacaact caccgccgtt ccgctttctt tccctctttt tatgtactcc
     2281 agtgattttt tggtgcttac cccggtgaaa tgtaatcttg atccggcgta acgggccagt
     2341 ttgatatccc tggctacaat caattcttct gccatggcgg gtttaccggg taaacccaat
     2401 tgagtggaga caatcccttc attcattaat ccatgtggat taatgctttt atcgtcgggt
     2461 aactgtatca ggataccgtc aaatgctttt acatattgca acgcttttac taaaagtccc
     2521 gcagactgca cacaatttat accgtcagaa aaggcggcgg caccacttga cttcatatca
     2581 tacatttctg ccaattcttt cccttcagtg tttttagtga tagcgcctat cggatgaata
     2641 ttgaccggga gtgatttgct tttatgtgtg atatattcaa cacctgcttt attatggatg
     2701 gagggatttg tattcggtat caccatcaca tctgtatagc cacctgctgc cgcagcggcc
     2761 gcccctgttt ccagggtttc cttgaactca taaccgggat ccgcaaaatt ggcaaacaca
     2821 tcaacccatc cgggagaggc atgaagcccc tctatcgcaa tttctttatc tgctttatct
     2881 ggtaaagcaa tacctatttt ggaaatgatt ccgttatcaa ttaaaatatc tgctgattgt
     2941 ccgttaaaag gagaggaggg atcaacaatt ctggcttgct taatgagtag cttcattaag
     3001 gggcgaagat aaagcttgta ttcggttcag aaaaaagata aaagcctgtg agaacaggct
     3061 ttaaatttag tcgggatgac tggattcgaa ccagcgaccc ctacgtcccg aacgtagtgc
     3121 gctaccgggc tgcgctacat cccgcaatga acaaccggtc agcaaaatta aaaaagaccc
     3181 ctgaaataat caggagtctt taaaaatgtc gggacgacta gattcgaact agcgacccct
     3241 tgcaccccat gcaagtgcgc taccgggctg cgctacgtcc cgaacaaacc gggctgcaaa
     3301 ggtaaagcta attagctaat cggcgaaacg aaactccttt gtctgtataa ctgaaaagtc
     3361 aaaacagatt tttttagaat tcattggtta ggtggtaaaa cttcacttac tttgcatcac
     3421 aaagtgcttt ttaaaaatta acaaacagaa tttgaaatat ttaaaagaga aatatttatg
     3481 gataggaaat caaatgttaa ggatatcatc agctggttat tcggaatagt cgttgttgcc
     3541 atcggcctgg tgaatttgtt ttggggtaac gacccgggtt ttggagtatt cctgctgcta
     3601 ctttctttgg tttactttct tccggtaaat gctattctcc ggaagctgac cggttttacc
     3661 atccctaaat tgggaatagt aaaaatcgtt ttgggcattt tcattctctg ggcttcgtta
     3721 ggggtgggtg aattgtttga taagatagaa ttgatgatga atagtttccg ggagtgaact
     3781 ctattgatgt gcatcaaatc aaaatgagtt tgatctgtgg tttgttctcc taaacaatat
     3841 cttgaatgtc atgctgaatc ttgaatgtca tgctgaatct tgaatgtcat gctgagcttg
     3901 tcgaagcatg accgaagcct gcctgtcggt aaacaagatt caccacaaag ggcactaagt
     3961 ttttacacag cgtacatagc gtttttcata ttgatgctgc gagaaactat agaactaaaa
     4021 acttcctccg tgtactttgt gatgtatttc ctccgtgttc tctgtggttg ataacactaa
     4081 acgtatctaa agataggctt caccacaaag ggcactaagt ttttacacag cgtacatagc
     4141 gtttttcata ttg
//
LOCUS       DAORTI010000218         4139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_27687_length_4139_cov_6.436337, whole genome shotgun sequence.
ACCESSION   DAORTI010000218 DAORTI010000000
VERSION     DAORTI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27687_length_4139_cov_6.436337"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1102)
                     /locus_tag="PLO70_16240"
     CDS             complement(68..1102)
                     /locus_tag="PLO70_16240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQZ76070.1"
                     /translation="MELGIGMFGDNHYNKDGTPQAPGQRLQELIEEIKLMDEVGLDFF
                     GIGEHHRPDYAVSAPEIILAAAATVTKNIKLGSAVSVLSSSDPVKLYQSFATIDLLSN
                     GRAELMAGRGSFIESYPLFGYRLEDYDELFEEKLELLLKINKENPVTWKGKHRAALKN
                     QQVLPRAVNDHLNIWVAVGGTPESVLRAGRYGLPVIFAIIGGNPAQFKPLFNYYKKVY
                     NHFEHAINNFQVGVHMHAFFGDNSKKTADEYYPIYSSQMDRVGSSRGWPPYQQQQYDM
                     GRGKNGHLIIGDADEAIDKILQLQELFGLTRFSAHMDVGGPSHASLLKSIEIYGTKIA
                     PKVREALNKR"
     gene            complement(1237..1659)
                     /locus_tag="PLO70_16245"
     CDS             complement(1237..1659)
                     /locus_tag="PLO70_16245"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HQZ76071.1"
                     /translation="MFLKILTVAGLATFEIYAAIPAGFAFGLSSVLIFLASLTGGLAG
                     AVVAAFFGDKIRAFFHKKKPATDETKKHPVITRLWNKYGIAGLGILGTITVGAPISIA
                     VGTGLNVNIKQLLVWCCLGVLIRCSLFTTIGHYGLQLL"
     gene            complement(1783..2445)
                     /locus_tag="PLO70_16250"
     CDS             complement(1783..2445)
                     /locus_tag="PLO70_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76072.1"
                     /translation="MHPENSNFHFRRPVQAACIAVFGLGVIYAVVTVLGLLSLPAPDE
                     QIADPYFTLMEVLILLIAPLMALVILAVHFYAAADKKWYSLAAAVFMFIMAAITSCVH
                     FTILTISRQPAAEQLAGHSFFFSFQWPSVVYALDILAWDWFYALSVFFAAAVFKTGRL
                     EKTIRTLLLVSGILSLVGLLGIPFQNMQIRNIGIIGYAVLGPVAFLFIGKWFGLSGKT
                     PD"
     gene            2727..3161
                     /locus_tag="PLO70_16255"
     CDS             2727..3161
                     /locus_tag="PLO70_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76073.1"
                     /translation="MRRVIIFLFAIGFLGLASQAQETNIKKERKRILKEQRQMETLNL
                     DGNQGQQVKAINSRYRTAHKEIMKNETLTQEQKQEQVQALNKRRVEEIHNIVGREKTQ
                     EWDRIKQQEKEKKEAIDKEKKEKKEKKIKKADKVNKGNKGRG"
     gene            complement(3312..3857)
                     /locus_tag="PLO70_16260"
     CDS             complement(3312..3857)
                     /locus_tag="PLO70_16260"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQZ76074.1"
                     /translation="MEFTLPFTIVNGGEKLTFLKSYFKDGVEVLEGINEVQPKAGPPM
                     HIHYLQDEGFTVVSGKMAYQLAGEPVQYAGPGDSVLIKAGIPHKFWNDGDDMLVVKGF
                     ITPPDNFIYFLSEIYKSIQANKGRPAMFDAAFLLNRYKSEYAMTEIPAFVQKMIFPVV
                     LFVGRLMGKHKKFAGAPLPVK"
     gene            complement(4028..>4139)
                     /locus_tag="PLO70_16265"
     CDS             complement(4028..>4139)
                     /locus_tag="PLO70_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQZ76075.1"
                     /translation="SALAAVALKQMGYTDVCHLDGGIKAWKEAGLPVHTD"
BASE COUNT         1308 a          923 c          858 g         1050 t
ORIGIN      
        1 tttttcctat taacaacggg atatacaacg acatgtcccg ttgctgacct ttaaacaaat
       61 tccattatca tcttttattc aacgcctctc tcactttggg ggcgatttta gttccgtata
      121 tctcgataga tttcaggagc gaagcatgtg aaggcccgcc tacatccatg tgtgcagaaa
      181 aacgggtgag accaaatagc tcctgcagtt gtaagatttt atcaatcgct tcatcagcat
      241 caccaattat taaatgcccg ttcttccctc ttcccatgtc atattgttgt tgctggtaag
      301 gtggccagcc acggctgctg cctaccctgt ccatctgtga ggaatagatg ggataatatt
      361 catcagctgt ttttttacta ttatcgccaa agaatgcatg catgtgcaca cccacctgga
      421 aattatttat agcatgttca aaatgattgt acactttctt ataatagtta aacaatggtt
      481 taaactgtgc cgggttaccg ccaataatgg cgaagatcac tggcaagcca taccggccgg
      541 cccgcaatac actctccggt gtaccgccaa ccgctaccca tatattcagg tgatcgttca
      601 cagcacgggg caatacctgt tggtttttta atgccgccct gtgttttcct ttccaggtga
      661 cagggttctc tttatttatt ttcaacagca gctccagttt ttcttcaaac aattcatcat
      721 agtcttccag cctgtaacca aacaaagggt acgactcaat aaaactgcca cgaccagcca
      781 tcagttctgc cctgccgttt gaaagcagat caatcgtggc aaaactttga tacaacttta
      841 ccgggtctga tgaacttaaa acagacactg cactcccgag ttttatattt tttgtgacgg
      901 tagctgcagc agctaaaatg atctcgggag cagataccgc atagtcaggc cggtgatgct
      961 caccgatgcc aaagaaatcg agtcctactt cgtccatcag ttttatttct tcgatcagtt
     1021 cctgcaaccg ttgacccgga gcctgtggcg taccatcctt attgtaatga ttatcgccaa
     1081 acataccgat gcctaattcc atattgtatt atttgaagac gaagatacaa gctgtgataa
     1141 tcctgcacta atcggaacgg tgtgtagatt atttcttccc ggtaaaggcc cgcttaaacg
     1201 caataacagt atttgctgtg gaaggcttcg atgtctttac aataactgca gcccataatg
     1261 acctattgtg gtaaataaac tacagcggat caacacaccg aggcagcacc agaccaatag
     1321 ttgtttgata ttcacattta acccggttcc aacagcaata cttatcggag cccccacggt
     1381 gatggtgccc aggataccca aaccggcaat gccatattta ttccagagcc ttgttataac
     1441 agggtgtttt tttgtttcat cagtggctgg tttcttttta tgaaagaaag ccctgatctt
     1501 atcgccaaaa aaagcagcca ccacggctcc tgcaagtccc ccggtgaggc tggccaaaaa
     1561 gatcagcacg gatgaaagac caaatgcaaa tccggcgggg atcgctgcat atatttcaaa
     1621 ggtagccaga ccggctaccg ttagtatttt caaaaacata gatacggtat aaataaatgt
     1681 ttgtgcaagg acagcaaaaa taatagaatc agggctgcga aatacaaaac tgcttgttaa
     1741 atcagcaaca acaactcctg cgaatagcgg ggattctatt tattagtcag gagttttccc
     1801 ggatagtcca aaccatttac caatgaataa aaaagcaact ggtcccagta cagcatagcc
     1861 aatgataccg atatttctta tctgcatatt ttgaaaaggt atacccaaca ggccaacaag
     1921 acttaatata cccgagacaa gaagcaatgt acggatcgtt ttctccagtc ttcctgtttt
     1981 gaatacagca gcagcaaaga acactgacaa ggcataaaac cagtcccatg ccaaaatatc
     2041 gagggcatat acaacagaag gccattgaaa agaaaagaag aaagaatgcc cggccagttg
     2101 ttccgcagca ggctgacggc taatagtaag aatagtaaaa tggacacagg aagtaatagc
     2161 ggccatgata aacataaaaa cagcagctgc caggctatac cattttttat ccgccgctgc
     2221 ataaaaatga acagccaaaa taacaagggc catcaacggg gcaataagca ggatcagaac
     2281 ttccatcagt gtaaaataag gatcagcgat ctgttcatcc ggggcgggca gcgacaataa
     2341 tcccaataca gtcacgaccg cataaataac ccccaggcca aaaacagcaa tacatgctgc
     2401 ctgtaccggt ctacggaaat gaaagtttga attttcggga tgcatagtat caaaagtgaa
     2461 ataaaattag atcaaatttt gaggcggtac aagccatgta acgggcattt aaaattatat
     2521 caccggtctg ggaattgaaa atttccggaa taataagcag atcggtaact aactgacaat
     2581 aatcacatag gctgccacta catatcataa taaaattatc ttgtctttaa taccttgaca
     2641 gtctcctgct ttaatcaaaa ttaaccctct cctgaccgct atgaaaagat ggtattgatt
     2701 tattattcac ttataaaatg aattttatga gaagggttat tattttctta tttgcgatcg
     2761 gctttttagg tcttgcctcc caggcgcagg aaacaaatat taaaaaggaa cgaaaacgca
     2821 ttttgaagga gcaacggcag atggaaacac tcaacctgga tggcaaccag gggcaacagg
     2881 tgaaagcgat caacagcagg taccgtacag cacataaaga gatcatgaaa aatgaaacgc
     2941 ttacacagga gcaaaagcag gaacaggtac aggccctgaa taaaagaaga gtagaagaga
     3001 ttcacaacat tgtcggaagg gagaaaacac aggaatggga tcgtatcaaa caacaggaaa
     3061 aagaaaagaa agaagccata gataaagaga agaaggaaaa gaaggagaaa aaaataaaga
     3121 aagctgataa agtgaataaa ggcaataaag gaagaggttg ataactatct ccagcttaag
     3181 caaattttga tgcggggcgg ttccgaaata ttcggagctc gggccatact ttttatcaat
     3241 aaaagctaga cggagaagac aaacaccaac cacggtggac aataaaaatt aattttcaca
     3301 aaccgccttt tttattttac cggcagcggc gcaccggcaa attttttatg cttgcccatc
     3361 aaccggccaa caaataaaac aacagggaat atcatttttt gtacaaaggc cggtatttcc
     3421 gtcatggcat attccgattt atagcggttc agcaaaaaag ctgcatcaaa cattgcgggc
     3481 cggcccttgt tggcctgtat ggatttgtag atctcggata aaaagtagat aaaattatcc
     3541 ggaggggtga tgaaaccttt caccaccagc atatcatcac catcattcca gaatttatgg
     3601 ggtatacctg ctttgatcag tactgaatca cccggaccgg catactgcac cggttcgccg
     3661 gccagctggt aagccatctt accggataca accgtaaagc cctcatcctg caggtaatgg
     3721 atatgcatgg gcgggccagc tttgggctgc acttcattaa tgccttccag cacttccacc
     3781 ccgtctttga aatagctttt taaaaaggtc agcttttcgc cgccgttgac gatcgtaaat
     3841 ggtaaggtga attccatagc aaagtatcgt ttgtttcggg gtttgacgct gatgccggaa
     3901 aaaagttact cctgcaaaga agttagggta agctaacggc cgtttacaac tggtaagccc
     3961 ttgttattca caatgcctgc atgacatttt gcacctattg gtgatccggc ctttgaaaaa
     4021 gaaaatatta atcagtgtgt acaggaagtc cagcttcttt ccatgccttg ataccaccat
     4081 ccagatgaca gacatcagtg tagcccattt gcttcagtgc aactgctgca agggctgac
//
LOCUS       DAORTI010000219         4093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28135_length_4093_cov_5.007925, whole genome shotgun sequence.
ACCESSION   DAORTI010000219 DAORTI010000000
VERSION     DAORTI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28135_length_4093_cov_5.007925"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLO70_16270"
     CDS             <1..200
                     /locus_tag="PLO70_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQZ76076.1"
                     /translation="ISSLKKKYNLSKDELEERPILGRLALHSFQLDFKDIDEKPIELE
                     AELPKDMRATLQQLRKRKGKK"
     gene            complement(201..1373)
                     /locus_tag="PLO70_16275"
     CDS             complement(201..1373)
                     /locus_tag="PLO70_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ76077.1"
                     /translation="MAKHPETNAVRIQTKKTGEKEHSTPLFLTSSFTYDSAEDMAAAF
                     SDDSLDVNIYSRFSNPSVDEFIEKIASLEGAEDGVATGTGMAAIFATFMTFLSKGDHI
                     ISASAVFGSTHTILTKYLPKWGIESSYFDMNKPETIEGLVKLNTKMLYVETPSNPGLD
                     IIDIEFVSALCKKYNILFVVDNCFATPAVQHPIKFGADLVVHSATKFIDGQGRVLGGV
                     VVGKKELIQPLYLFIRNTGPSMSPFNAWVLSKSLETLFIRMERHAASAMQIAKALNGH
                     EKLSSVKYPLLETHPQYVIAKKQMSNGGGIVTFELKGGIEAGRNFLNSLQMISMTNNL
                     GDSRTIASHPASTTHSKLSLEERAAVGITDGLIRISVGLENAEDILSDILQALGKS"
     gene            complement(1439..2464)
                     /locus_tag="PLO70_16280"
     CDS             complement(1439..2464)
                     /locus_tag="PLO70_16280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQZ76078.1"
                     /translation="MIYSEEFINKFSAVTDEVTVAEPVIEKNKLLKIEEQADLTEEIH
                     GNIRNERKWKRLATVLLVSFALMIIGFFVYQYFGYATLKSLINSATQSIDTSFYLFIG
                     AGFIAQMVDGAVGMGYGVTSTSILMSMGVHPAAISGSVHTAEIFSSAASGYSHYKFGN
                     VNKKLFKVLVIPGIIGAIVGAGALVWLGDKYGNMIKPFLAAYCLLLGIRILYQAYKRE
                     KKIKKVKKAGWLAGAGGFLDSFGGGGWGPLVTSTLIAKGRSPQYVIGSVSLSEFFVTF
                     FSALSFFVFIGITHWQVIAGLMIGGAIAAPIAAKLAGKLPLRTMLICVGIMVILWSLR
                     ILIKALA"
     gene            complement(2472..3713)
                     /locus_tag="PLO70_16285"
     CDS             complement(2472..3713)
                     /locus_tag="PLO70_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQZ76079.1"
                     /translation="MDLLRFITAGSVDDGKSTLIGRLLYDSKNILVDQLEALEKSTKN
                     RTDGSVDLALLTDGLRAEREQGITIDVAYRYFTTPKRKFIIADAPGHVQYTRNMITGA
                     SNASLIIILIDARQGVVEQTRRHSIIASLLKIPHVVVAVNKMDLVNYSQDVYNNILID
                     YAKVAKQLGLHDVTYIPISALNGDNIVDKSTTIRWYEGKPLLEMLEEVEVNDDVNLTD
                     ARFQVQLVLRPQTEELHDYRGYAGKVLSGIYKKGDAVTILPSGIKTTISQLEVGGKEV
                     EDVFAPQSVTIQLSDDIDISRGDMIVKSDNLPQVSNELDVLICWVDDKPLQAGNKYLL
                     QHNSRLVRTVVKSIEYKLDVNSLEEQPVNDGVRLNEVVKATIKTASPLVYDSYSTLSE
                     NGSAILIDETSNSTVAAVLFK"
BASE COUNT         1240 a          964 c          624 g         1265 t
ORIGIN      
        1 taatttccag cttgaagaag aaatataatc tctcaaaaga tgaactggaa gaacggccaa
       61 ttcttggcag attggcatta cattcttttc aactggattt taaagatatt gatgaaaaac
      121 ccattgaact ggaagcagaa ttacctaaag atatgcgggc aacattgcag cagttgagga
      181 agaggaaagg aaagaaataa tcaactcttc cccaacgcct gcaatatatc actcaatata
      241 tcttctgcat tttccaatcc cacagaaata cgaatcaaac catctgtaat accaacggct
      301 gctctttctt ccaagctaag tttagaatgt gtggttgagg caggatgcga agcgatggtg
      361 cggctgtctc ccaagttgtt cgtcatcgaa atcatttgta aactattcag aaaatttctt
      421 ccagcttcaa tgccgccttt taattcaaaa gttacaatgc caccgccatt gctcatctgt
      481 ttctttgcaa taacatattg cggatgtgtt tccagcaatg gatattttac agaactcagt
      541 ttttcatgtc cgtttaatgc tttcgcaatc tgcattgcac ttgccgcatg tcgttccatt
      601 cttataaata atgtttccag gctttttgat aatacccaag cattaaaagg actcatggat
      661 ggccctgtat tgcggataaa aagatatagt ggttgtatca gttctttttt gccaacaact
      721 actccaccta atacccttcc ttgaccgtca ataaattttg tagcagaatg tacaacaagg
      781 tcggcaccaa atttaatcgg gtgctgcact gccggagtgg caaaacaatt atcaactaca
      841 aaaagaatat tgtatttctt gcataatgca gatacaaatt caatatcaat aatatccaaa
      901 ccaggattac ttggggtttc cacatacagc atttttgtat tcagctttac caatccttca
      961 atcgtttccg gtttgttcat atcaaaataa gaagactcaa ttccccactt aggtaaatat
     1021 tttgtaagaa tagtatgtgt ggaaccaaac acagccgatg cagaaataat atgatcgcct
     1081 ttacttaaga aagtcatgaa cgttgcaaaa atggccgcca tacctgtacc ggtagccaca
     1141 ccatcttcgg ctccttccaa tgaagcaatt ttttcaataa actcatccac actgggattg
     1201 gaaaaacggg aatagatatt gacatctaat gaatcatctg aaaatgcagc cgccatatct
     1261 tccgctgaat cataagtaaa actgctcgta agaaacaatg gagttgaatg ttctttctca
     1321 cccgtcttct ttgtttgaat tcttactgca tttgtttcag gatgctttgc catacaaatt
     1381 atctgttgat tttattaaag caaatataca tccatgagct ttgggagact cattataatc
     1441 aagctaaagc tttgattaaa attctaagac tccagagaat aaccatgatg cccacacaga
     1501 tcaacatagt tctcaatggt aatttgcctg caagctttgc agcaatggga gcagcaatgg
     1561 ctccgcctat catcaatccg gcaattacct gccaatgcgt aatgccaata aatacaaaga
     1621 aggataatgc actaaagaag gttacaaaaa attcactcag gctcacactg ccgattacat
     1681 attgcggact tctgcctttt gctatcaatg tacttgttac caaaggtccc cagccaccgc
     1741 caccaaaact gtcgagaaaa ccacctgcac ctgcaagcca accagctttc tttactttct
     1801 taattttctt ttctctttta tacgcctggt ataatattct tatccctaat aataaacaat
     1861 aggcagcaag gaaaggcttt atcatattac cgtatttatc acccaaccac actaatgcac
     1921 ctgcgccaac tattgcacca ataattccag gtatcactaa tactttaaat agtttcttat
     1981 ttacgtttcc aaatttataa tggctataac cgcttgctgc actgctaaaa atttccgccg
     2041 tatgcacact gccactgata gccgccggat gcacacccat actcattaaa atactggtgc
     2101 tggtaacacc atagcccatt cccaccgcac catccaccat ctgtgcaata aatccggctc
     2161 ctataaataa atagaaagaa gtatcaatac tttgtgttgc tgaatttatt aatgacttca
     2221 atgttgcata gccaaaatac tgatacacaa aaaatccaat tatcattaat gcgaatgaaa
     2281 caagcagcac tgttgccagg cgtttccatt tccgttcatt cctgatattg ccgtgaatct
     2341 cctccgtcaa atcagcctgc tcttcaattt ttaataattt attcttttca ataacaggtt
     2401 ctgctaccgt cacttcgtca gtaaccgcag aaaacttatt tataaattct tcagaataaa
     2461 tcataaattt tttacttaaa caatacagcc gctactgttg aattacttgt ttcatctatg
     2521 agaatagcac ttccattttc acttagtgtt gaatacgaat catacaccaa aggagatgca
     2581 gttttaatcg tggccttcac aacttcgtta agtctcactc catcatttac tggttgctct
     2641 tccaatgaat taacatcaag cttgtattca atacttttta ccaccgttct caccaatcgg
     2701 ctgttatgtt gcagcaaata tttattgcca gcttgcaaag gcttatcatc cacccagcat
     2761 atcagcacat ccaattcatt acttacttgt ggaagattat ccgattttac aatcatatca
     2821 ccacggctta tatctatatc atcacttaat tgaatcgtta cgctttgtgg cgcaaacaca
     2881 tcttccactt cttttccacc cacttccagt tgactgattg tagttttaat tcccgaaggc
     2941 aatatcgtaa cggcatcccc ttttttataa atgccactca atacttttcc tgcatagcca
     3001 cggtaatcat gtagttcttc tgtttgcgga cgcaacacca actgcacctg aaaacgggca
     3061 tccgttaaat tcacatcatc atttacttct acttcttcca gcatttccaa taaaggtttc
     3121 ccttcatacc agcggattgt tgttgacttg tcaacaatat tatcgccatt caatgcactg
     3181 ataggaatat aggtcacatc atgtagtccc aattgcttcg ccaccttcgc atagtcaatc
     3241 aaaatattat tataaacatc ctgcgaataa ttcaccaagt ccattttatt cactgccacc
     3301 acaacatgcg gaatttttaa cagcgatgca ataatagaat gccttctcgt ttgctccacc
     3361 acaccttgtc ttgcatcaat caaaataata atgaggcttg cattcgatgc acctgttatc
     3421 atatttcttg tgtattgcac atgccccggc gcatcggcga taatgaattt tcgtttcggt
     3481 gtagtgaaat agcgatacgc cacatcaatg gtaatacctt gttcccgttc tgcccgcaaa
     3541 ccatccgtca gcaatgccag atcaacagaa ccatccgtcc ggttctttgt tgatttttcc
     3601 aaagcttcca attgatctac caaaatattt ttactatcat acagcaaccg accaatcaat
     3661 gtgcttttgc catcatctac acttcctgcg gttataaaac gtaatagatc catacacctg
     3721 tcccctaaag gggaacttag ttttgtatta gccttactat ttgcaagact aagaaattaa
     3781 aaatattatg tagccagctt cagtactact atcatcgtcc cttgcgtcgc actcttgtac
     3841 tgttgtaatt ctattcatca atacatctca cctcccgaaa aatcgggaca agctctacgg
     3901 ggttcaaata cattcttcgt tttaatgtct ccaagttaaa accccggagc tagtaacatg
     3961 tcatctccca aaaaaatcgg gacaggctct acggaactaa aagaaaaaag taatctcatt
     4021 tatctcaaat tttttcttct cgacttccag tcttcccggc tatttaaaaa tatccattct
     4081 tctttctgtc ttc
//
LOCUS       DAORTI010000220         4088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28188_length_4088_cov_5.198611, whole genome shotgun sequence.
ACCESSION   DAORTI010000220 DAORTI010000000
VERSION     DAORTI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28188_length_4088_cov_5.198611"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLO70_16290"
     CDS             <1..290
                     /locus_tag="PLO70_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ76080.1"
                     /translation="KNENLLIISMAGIGLAWCSILAMPYSMLTKALPANKMGVYMGIF
                     NFFIVIPQILAATLLGIFTKHIFNGNAIMTIVLGGCSMIVAAILTLRIKDK"
     gene            296..2173
                     /locus_tag="PLO70_16295"
     CDS             296..2173
                     /locus_tag="PLO70_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQZ76081.1"
                     /translation="MRKIFFVILLAAGLSVQGTDMYGVYPTHWWIGMKNPKVQLLLHG
                     ENIGLFNKATISYAGIKVQKVTRLESNNYLVIDLLISPSAKPGTFKIVVSGGAPGAED
                     IMYELKPRSKENGKTRVKGVNAKDFIYLLMPDRFSNGDPSNDVIADYRDKSCDRKDKF
                     SRHGGDFKGIESKLDYLDQLGVTTVWLTPVIENDMPKMPEWGNNVAGYHGYWFTNHYA
                     IDKRFGGNEAYKNFCNTLHAKGMKVIQDAVYNHVGNHHWFALDPPMKSWFNTSQGDKG
                     PNHREEVFYDPYASQQDKVQMLEGWFVPHLPDLNQRNPYVANFLIQHAIWTTEEYGID
                     GWRVDTYKYCDEQFMNKVNTALEKEFPAVTVFGESWVNTTVANAYFTQNNINAPFRHN
                     AEGMLDFQTCFAMLAGMNESFGWQNGVAKIYTTLTHDVLYKNPMNNCTFLDNHDMDRV
                     FSTVNEDWKKLKMGINWLLTIRGIPQLYYGTEVLMKNKKEGSDAPVREDFPGGWPDDN
                     PGNNRFTKEGRSEQQNEALHYISVLANFRKKSSALTTGKTMQFVPKDGMYVYFRYDNR
                     QTVMVITNSGDKAVKPDWIPFEERMKGFTRVKNVVNGHIEHLSDLILEPNESFVFELL
                     Q"
     gene            2213..>4088
                     /gene="glgB"
                     /locus_tag="PLO70_16300"
     CDS             2213..>4088
                     /gene="glgB"
                     /locus_tag="PLO70_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQZ76082.1"
                     /translation="MAKAKKTRESRDNVIGETEVKKIGISAAKYEEANFVDTTKPVWN
                     YSLLTDEDVANYQNGTNYQLYKKFGSHSIQVNNVWGMYFCVWAPNGTSVSVKGNFNDW
                     KDHEFELYPRWDKSGIWEGFIPHFKLGEAYKYHIIGYAKRKLDKGDPFANFWEKRPQT
                     ASITWDMYYEWKDDEWMKKRKKHNSLDAPWSVYEVHLASWQRPDKNDEETYNSYDEIR
                     ERLVPYVKETGFTHVEFMPVMEHPFDGSWGYQCTGFFASTSRFGDPQGLMRLIDAFHQ
                     EGIGVILDWVPSHFPYDAHGLFMFDGTHTYEYADMRKGFHPDWNSYIFNYKRGEVKSF
                     LISSARYWFDLFHIDGIRVDAVSSMLKLNYSREHGQWEPNEFGGDGNLEAIAFIKDLN
                     ETIFRDFPDVQTIAEEATDWPGISKPTWQDGLGFGMKWMMGWMHDTLDYFEIDPINRQ
                     FHQDKFSFSMMYYYDENFMLPLSHDEVVHGKSPMIYKVPGDEWQKFANLRLLYTYMWT
                     HPGAKLLFMGNEFGQTAEWNYKSELQWELLQFDCHRLLKNCVADLNKLLQSEPALHHN
                     QFKMDGFEWVDLNHRAETVIVFKRKGKKKGDDLLIILNMTPVVRNDWPIEVIGKAYTE
                     E"
BASE COUNT         1277 a          707 c          970 g         1134 t
ORIGIN      
        1 ttaaaaatga aaacttgctc attatcagta tggccggaat aggactggca tggtgcagta
       61 tcctggcaat gccttattca atgcttacaa aggccttacc agctaataag atgggtgtgt
      121 atatgggcat attcaatttc tttattgtga ttccacagat actggctgct acattacttg
      181 gaatttttac caagcatata tttaatggta atgcgatcat gaccattgtt ttgggcggtt
      241 gttcaatgat tgttgcagcc attcttacct tacgaataaa agataaatag catttatgag
      301 aaagatattt tttgtcatac tgttagcagc agggttgtct gtacagggaa ctgatatgta
      361 cggtgtttat cctacgcatt ggtggatagg aatgaaaaac ccaaaagtcc agctcttatt
      421 gcatggagag aatattggtt tatttaataa agcaacgatc agttatgccg gtataaaagt
      481 tcaaaaagtt accaggctgg aaagcaataa ttacctggtc attgatttgt tgatcagtcc
      541 ctccgctaaa cccggtacat tcaaaatagt tgtttcagga ggtgcaccgg gtgcagagga
      601 tattatgtat gaattaaaac cccgaagtaa agagaacggg aaaacaagag ttaaaggtgt
      661 taacgcaaag gattttattt acctgctgat gcctgaccgc ttttctaacg gagatccttc
      721 aaatgatgta attgcagatt acagggacaa atcatgtgac cggaaagata agttttcccg
      781 tcatggcggc gatttcaaag gcattgaaag caaactggat tacctggatc agttaggcgt
      841 tactaccgta tggcttacac cggttattga aaatgatatg cccaaaatgc cggagtgggg
      901 caataatgta gccggttatc atggctactg gtttaccaat cattatgcaa tagacaagcg
      961 gtttggcggg aatgaagcgt ataagaattt ttgtaacaca ttgcatgcaa aaggaatgaa
     1021 agtgatacag gatgcagtgt ataatcatgt gggcaatcat cactggtttg cactggatcc
     1081 gccaatgaag agttggttca atacatcaca gggtgataaa ggtcccaatc accgcgaaga
     1141 ggttttttat gatccttatg cttcccaaca agataaagta caaatgctgg aaggatggtt
     1201 tgtaccgcat ctgccggatt tgaatcagcg taatccttat gtggccaatt tcctgataca
     1261 gcatgctatc tggacaacgg aggaatatgg aattgacggc tggagggtcg acacttacaa
     1321 atattgtgat gagcaattca tgaataaagt gaatactgcg cttgaaaagg aattccctgc
     1381 tgtcaccgtt tttggagaat cctgggtgaa tacgactgtg gcgaatgcct atttcactca
     1441 aaacaatatt aatgcacctt tcaggcataa tgcagaaggt atgcttgact ttcaaacatg
     1501 ttttgccatg ctggccggaa tgaatgaatc ttttggctgg caaaatgggg tggcaaaaat
     1561 ttataccacc ttgacgcatg atgtactcta taaaaatccg atgaacaatt gcactttcct
     1621 ggataatcat gacatggaca gggtcttttc cacggtgaat gaagactgga aaaaattaaa
     1681 gatgggaatc aactggttgt taacaatccg gggcataccg caactgtatt atggaactga
     1741 agtactgatg aaaaacaaga aagaaggttc tgatgcacct gtgcgggaag atttccccgg
     1801 cggctggccg gatgataatc cagggaacaa tcgctttaca aaagagggaa ggagtgaaca
     1861 acaaaatgaa gcacttcatt atatcagtgt cctggccaat ttcaggaaaa agtcctccgc
     1921 attgaccact ggtaaaacca tgcaatttgt tccaaaggat ggcatgtatg tttacttccg
     1981 ttacgataac aggcaaaccg ttatggtcat taccaactca ggtgataaag cggttaagcc
     2041 tgactggatt ccttttgaag aaagaatgaa aggttttacc cgggtgaaga atgttgtaaa
     2101 cggacatata gagcatttaa gtgatcttat tctagaacca aatgaaagtt ttgtatttga
     2161 attactgcag taagattggc taatttctgc attctttggt ttatatttaa gtatggcgaa
     2221 agcaaagaaa acccgggaat ccagggataa tgttattgga gaaactgaag ttaaaaaaat
     2281 aggaatctcc gcagcgaaat acgaagaagc aaatttcgta gacactacaa agcctgtctg
     2341 gaattattcc ttgctcactg atgaagatgt tgccaattat caaaatggta ccaattatca
     2401 gctctacaag aaatttggtt cacattccat tcaggtaaac aatgtttggg gaatgtattt
     2461 ctgtgtatgg gcacctaacg gaacttcagt ttctgttaag ggcaacttca atgactggaa
     2521 agatcatgag tttgaattgt atccccgctg ggataagagt ggtatctggg agggttttat
     2581 tcctcatttt aaattagggg aagcttacaa gtaccatatt atcgggtatg caaaaagaaa
     2641 gctggataaa ggtgatccct ttgccaactt ctgggaaaaa agaccacaga cggcaagtat
     2701 tacctgggat atgtattatg aatggaagga tgacgagtgg atgaagaaaa gaaaaaagca
     2761 taattcactg gacgcaccat ggagtgttta tgaagtacac ctggcaagct ggcaacgtcc
     2821 ggataagaat gatgaagaaa cctacaatag ttatgatgag ataagagaac ggttagttcc
     2881 ctatgtaaaa gaaacgggat ttacacatgt ggagtttatg ccggtgatgg aacatccgtt
     2941 tgacggaagc tggggctatc aatgcaccgg tttctttgct tccacatcaa ggtttggtga
     3001 tccgcagggc ctgatgcggc tgattgatgc gtttcaccag gaaggcatcg gtgtgatatt
     3061 ggactgggtg ccttcgcatt ttccatacga tgcacatggg ctcttcatgt ttgacggtac
     3121 tcatacttat gaatatgccg atatgcgcaa aggatttcat cctgactgga acagttacat
     3181 atttaactat aaaaggggag aagtaaaatc attcctgata agcagtgcca ggtattggtt
     3241 tgatctgttt catattgatg gtatccgggt ggatgcagtc tcatccatgc tcaaactgaa
     3301 ttattccagg gaacatgggc aatgggaacc aaatgaattt ggcggcgacg ggaacctgga
     3361 agccatcgca tttataaaag acctgaatga gacaatcttc agggattttc ctgatgtgca
     3421 gaccattgca gaagaagcaa cagactggcc gggtatatca aaacccacct ggcaggatgg
     3481 tttgggattt ggtatgaaat ggatgatggg ttggatgcat gatacattgg attattttga
     3541 gatagatcct attaacaggc aatttcacca ggacaagttt tctttcagca tgatgtatta
     3601 ctatgatgaa aatttcatgc ttccgttaag tcatgatgaa gtggtgcatg gcaaaagccc
     3661 catgatctat aaagtaccgg gggatgaatg gcagaagttt gcgaacctgc gattattata
     3721 cacttacatg tggacacatc cgggtgctaa attactattc atgggtaatg aatttggcca
     3781 aacggctgag tggaattata aatcagaact gcagtgggaa ttactgcaat tcgattgtca
     3841 ccggttacta aaaaattgtg ttgctgatct taacaagtta ttgcaaagcg agccggcttt
     3901 gcatcataat cagttcaaaa tggatgggtt tgaatgggtg gacctgaatc atcgtgcaga
     3961 aactgtgatt gtgtttaaaa gaaaaggaaa gaaaaaagga gatgatctgc ttatcatact
     4021 gaatatgacc cctgtagtcc gtaatgactg gccaatagaa gtgatcggca aagcttacac
     4081 agaagaaa
//
LOCUS       DAORTI010000221         4079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28291_length_4079_cov_6.733101, whole genome shotgun sequence.
ACCESSION   DAORTI010000221 DAORTI010000000
VERSION     DAORTI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28291_length_4079_cov_6.733101"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLO70_16305"
     CDS             complement(<1..1184)
                     /locus_tag="PLO70_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQZ76083.1"
                     /translation="MAVIFYTIFLWLFKGSTHIASLFNPKAAKWVTGRKNIFKRLKEA
                     IPQNEKTIWMHCASLGEFEQGRPLLEKLRLQYPDYKLLLTFFSPSGYEVQKNYPGADW
                     IFYLPMDGPKNAKRFLEIVHPSLVIFVKYEFWYYYLKKIKYRNIPLLLVSALFRKDMS
                     FFKWYGGLQRKMLSRFNHLFVQTPESKKLVDEIGLRDICTVSGDTRFDRVIEIASAFE
                     PIPAIGDFIGNSRAIVAGSTWPEDEEVLQKVFSAINDPSLKLIIAPHEINEKHLDDIC
                     ILFPAAVRFSSLTKPGQEISNILIIDNIGMLSRLYKYACFTYVGGGLSKNGVHNVLEA
                     AVYNKIVFFGPYYYKYTEAVGLVKSGGGISFTDEKRDGNMLRLLIEALLNEEDEFKFR
                     SNA"
     gene            1282..2412
                     /locus_tag="PLO70_16310"
     CDS             1282..2412
                     /locus_tag="PLO70_16310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQZ76084.1"
                     /translation="MRQIQMVDTRTQYHKIKAEVDKAVLDVMESSAFINGKPVQDFAD
                     GLSSYIGVKHTIPCANGTDALQIAMMALGLQPGDEVITPSFTYIATTEVIALLQLKPV
                     FVEVDAKTFCIEPAAIEKAITPKTKAIVPVHLYGHAAPMEEILAIAKKHNLFVIEDNA
                     QAIGCNYTFSDGITKKTGAIGHIGCTSFYPSKNLGAFGDGGAIFTNDDELAVKLKMIA
                     NHGQSKRYYHDVVGCNSRLDTIQAAILDIKLKHLDEYITARKKAADFYDKAFAGHAKI
                     TTPYRAVYSDHAFHQYTLILDGVDRDGLNLYLSEQHVPSMIYYPVPGHKQKMFEQFNT
                     ASQEMPVTDWLTERVISLPMHTELDEEQLDFIASNVLNYINK"
     gene            2454..3743
                     /locus_tag="PLO70_16315"
     CDS             2454..3743
                     /locus_tag="PLO70_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQZ76085.1"
                     /translation="MYQELIDKKAKLAVIGLGYVGLPIALEFARKISVIGFDISAKRI
                     EMMKNGIDPSNELEKEAFEDCDITFTDSLDVLREAKFFIVAVPTPVDEHKVPDLTPVQ
                     KASETVGKVIKKGDYVVFESTVYPGCTEEDCLPIIEKLSGLKNVVDFKSGYSPERINP
                     GDKNHTLATIVKVVSGCDAESLEEVAKTYELVVKAGVHRASSIKVAEAAKIIENTQRD
                     LNIALMNELSVIFDKMNINTYEVLEAAGTKWNFLKFQPGLVGGHCIGVDPYYLTYKAK
                     ELGYHSEVILAGRNINDNMGKYVAKKVIQHIIKNNGDVKSSKVLIMGATFKEDVSDIR
                     NSKVSDVVNALKEFYVNVEVQDPYADSDELKHEYGFGLVKEIANDYDAVIIPVPHREY
                     KELDENFFAGITKTHGMIADLKGIFRNKITNRTYWSL"
     gene            3745..>4079
                     /locus_tag="PLO70_16320"
     CDS             3745..>4079
                     /locus_tag="PLO70_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HQZ76086.1"
                     /translation="MPFIKTEFSGLFVFEPVVFEDSRGYFFESYNENIFQQQGIYTRW
                     VQDNQSSSAYGVIRGLHYQLPPNDQAKLVRVLRGKILDVVVDIRKGSPTYSKVFSIVL
                     SAKNKRQLLV"
BASE COUNT         1279 a          720 c          871 g         1209 t
ORIGIN      
        1 gcattacttc tgaatttgaa ttcatcctct tcatttaata atgcttctat tagcaagcga
       61 agcatattac cgtctctttt ttcatctgtg aatgaaatcc cacctcctga ttttacaaga
      121 ccgactgctt ctgtatactt gtaataatac ggtccaaaga atacgatttt gttataaaca
      181 gctgcttcca gtacattatg tactccattt ttggaaagcc cgcctccaac ataagtgaag
      241 caggcatatt tgtataatct tgaaagcatt ccgatgttat cgataataag aatattggag
      301 atttcctggc caggttttgt tagtgacgag aatctgacag cggccgggaa aagtatgcat
      361 atatcatcca ggtgtttttc attgatctca tgcggggcaa taataagttt gagggaaggg
      421 tcgttgatcg ctgaaaatac tttttgtaat acttcttcat cttcaggcca ggtgctgcct
      481 gctacgatgg ccctgctgtt tccaataaaa tcgccgatag ccggaatggg ttcaaacgct
      541 gatgcaattt caattacccg gtcaaaccgg gtgtccccgg aaacagtaca tatatcccgc
      601 aagccaattt cgtctaccag tttttttgat tccggtgttt gtacaaaaag atgattaaaa
      661 cgggaaagca tttttcgctg gagccctcca taccatttaa agaaagacat atcttttctg
      721 aataaagcag ataccagcag taaaggaata ttcctgtatt ttatcttttt gagatagtaa
      781 taccagaatt catatttcac aaaaatcact aatgaggggt ggacgatttc caggaagcgt
      841 ttggcatttt ttggcccatc cataggtaaa tagaaaatcc aatcggcgcc aggataattt
      901 ttctgcactt catagccaga aggggagaag aaagtcaaaa gaagtttata atccgggtat
      961 tgaagtctta atttttccag caaaggccta ccctgttcaa attcaccaag ggaggcgcaa
     1021 tgcatccata tagtcttttc attttgagga atagcctctt taagccgttt aaatatattt
     1081 tttctgcctg tcacccattt tgcagctttg ggattgaaca aagaagcaat atgggtgctg
     1141 cctttaaaca gccaaagaaa aatagtatag aaaataacgg ccaaaacgct ggaattgaat
     1201 tggtaacaaa tgtaaaagca ttcgggcaaa ctacctgtat tttactattt ttgcggcact
     1261 ttactagaaa taattggttc tatgcgacaa attcagatgg tagatacccg gactcagtat
     1321 cataagatta aggcggaagt agataaagct gttttagatg tgatggaaag ttctgctttc
     1381 atcaatggta agcctgttca ggatttcgct gatggcctgt cgtcttatat aggtgtaaaa
     1441 catacaatac cttgtgctaa cggtacagat gcattacaga ttgctatgat ggctttaggc
     1501 ttacagccgg gtgatgaagt aattactccc tcttttacat atatcgctac tactgaggtg
     1561 atagcattgc tacagctgaa accagtattt gtagaagtag atgcaaaaac tttctgtatt
     1621 gaacctgcag ctatagagaa agcgatcact ccaaaaacaa aagctattgt tccggtacat
     1681 ctttatggcc atgccgcacc gatggaggaa atattggcca ttgcaaaaaa gcataacctg
     1741 tttgtgatcg aggataatgc ccaggctatt ggctgtaatt atacattttc agatggtata
     1801 actaaaaaaa cgggtgctat cggtcatatt gggtgtacct cattctatcc atccaaaaat
     1861 cttggggctt ttggggatgg cggtgccatt tttactaatg atgatgagtt agcggtaaaa
     1921 ttgaaaatga ttgctaatca tggtcaaagt aaaagatatt atcatgatgt ggtaggatgc
     1981 aacagcaggc tcgatacaat ccaggctgct attcttgata tcaagctgaa acacctggat
     2041 gagtacatta ctgcaagaaa aaaagctgct gacttttatg ataaggcttt tgccggtcat
     2101 gctaaaataa caactcctta cagggctgtt tattctgatc atgcttttca ccagtatact
     2161 ttgattttag atggggttga ccgtgatgga ttgaacctgt acctgtctga acagcatgtg
     2221 ccttcaatga tctattaccc ggtgcccggg cacaagcaga aaatgtttga acagtttaat
     2281 acagcttccc aggaaatgcc agtaactgac tggctcactg aaagggtaat ttcattaccc
     2341 atgcacactg agcttgatga ggagcaatta gattttattg catcaaatgt gctgaactat
     2401 atcaataaat aatttaagca ttctttactt atttttttaa accttaacga aaaatgtacc
     2461 aggaactaat tgacaaaaaa gctaagttgg ctgttatcgg attaggctat gtgggactcc
     2521 ctattgcttt ggaatttgcc cggaagatat ctgttatcgg ctttgacatc agtgcaaagc
     2581 ggatagaaat gatgaaaaat ggtatagatc cgagtaatga attggaaaag gaggcttttg
     2641 aagattgcga tataacattt acagattcac tggatgtatt gcgggaagca aaatttttca
     2701 tagttgctgt tcctacaccg gtagatgagc ataaagtgcc agatctgaca cccgttcaaa
     2761 aagcatccga aacggtaggc aaagtgatta agaaaggtga ttatgtagtg tttgaatcaa
     2821 ctgtttatcc cggatgtaca gaagaagatt gcctgcctat tatcgaaaag ttatcaggat
     2881 taaaaaatgt tgtggacttt aaatcaggct attcacccga acgtattaac ccgggagata
     2941 aaaatcatac attggcaaca atcgtaaaag ttgtttccgg ttgtgatgcc gaatcactgg
     3001 aagaagtagc aaaaacgtat gaattggtgg ttaaagcagg cgtacacaga gcgtcttcta
     3061 tcaaagtagc agaagctgcc aagatcattg agaatacaca acgggatctg aacattgcat
     3121 tgatgaatga gttatctgtc atttttgaca agatgaatat aaatacctat gaagtactgg
     3181 aagcagccgg aacaaaatgg aattttctga aatttcagcc cggcctggta ggaggtcatt
     3241 gtattggtgt ggatccctat tacctgacat ataaagcaaa ggaactcggt tatcattctg
     3301 aagtaatttt ggcaggtcgt aatatcaatg ataatatggg taagtatgta gctaagaaag
     3361 taatacagca tattattaaa aataacggcg atgttaaatc atccaaagtg ctgatcatgg
     3421 gtgctacttt taaggaggat gtaagtgata tccgaaattc caaagtgagt gatgtcgtga
     3481 atgcactgaa agaattttat gtaaatgtgg aggtgcagga tccctatgct gattcagatg
     3541 aactaaagca tgaatacgga tttggtttgg tgaaagagat cgcaaacgat tacgatgctg
     3601 taattatccc ggtaccacat agagagtaca aggaactgga tgaaaatttc tttgcgggaa
     3661 taaccaagac ccatggtatg attgcagatc tcaaaggaat ttttcgtaat aaaattacta
     3721 accgaacata ctggagtttg taatatgcca tttattaaaa cagaattttc aggattattt
     3781 gtctttgaac ctgtcgtatt tgaagatagc cgtggttact tctttgaatc atacaatgaa
     3841 aatatttttc agcagcaggg aatctacacc cgctgggttc aggataacca gtcttcgtca
     3901 gcttacggag ttatacgggg tctgcactat cagcttcctc caaatgacca ggcaaaattg
     3961 gtaagagtgc tgcggggtaa aattctggat gttgttgtgg atatacgtaa gggttcgcca
     4021 acctacagta aagtattcag tatagtattg tctgcaaaaa ataaacggca attattagt
//
LOCUS       DAORTI010000222         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28381_length_4072_cov_4.561613, whole genome shotgun sequence.
ACCESSION   DAORTI010000222 DAORTI010000000
VERSION     DAORTI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28381_length_4072_cov_4.561613"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3051)
                     /locus_tag="PLO70_16325"
     CDS             complement(<1..3051)
                     /locus_tag="PLO70_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ76087.1"
                     /translation="MLLCFAGTAVLAQLHYKGRNLTITDGLSDNRVTCFYKDRSGFLW
                     VGTKNGLNRYDGHSFKIFRPAKENSISNEVINDIAGDSEGRIWVATMGGLNCYDPADN
                     SWNVLLPGARKTKNEIPNLLIWDIWFDNKGLLWIASDVFEFTSYDTKNKKFTYYDWPG
                     FAREKLQFANAGNYNSIQKFVAKGEQEFLLGTSKGLVSLQTETGEFTFLGGKYYADVY
                     DIKYDPGSRRVFLSVQNGQVFSYDEQTKDYVELKQEVEAYPSIEFKSLSQTESWMASE
                     KGLLKIDPVKNKAILSSPTLPFPGALLPGGVTSIMTDETGIRWIATPNGITSIDYSHL
                     YSYFLPLVLSSGKEGINYLTGVYFDAEVDKYFVCSANPASVFIISRKTGQIERITKDQ
                     SGAALPACFTVKKDAGNRLWLLTERNVYLYDRKGRSFSLFPLPNKGEVAGFRDVIQDA
                     EGNLWFAGFQAGIYYYNTAAKKCIDIPDSLGKYLNRLGTCLYSDSINKEVWIGTMGAN
                     LYKYDLVAKKMVSYYETEQTRDYASLNLINDITADQNGTIWLATHTGGVFRYNRGQSY
                     TKAFTKFDMRTGLESNNFISLAADQKNNLWLLSGNGISVMDTAGRPLSSFTNERTFTF
                     SSYGSDVNIPHDIFYDKARKELVLAAAGGVLFYYPYPEQNTITFPLILTEVVVEGAQQ
                     NLKPLHTGSALRIPSGTRSLQLQFAGLYFGGSDEILYEYRLKGHDTGWIRTDNNFRIS
                     YQNLPAGTYTFQARAVDRFGRIAGELKEIPVSVSPAFWQTGWFLVLCILAVLAAVVGI
                     IHSLQQKLKEEEQLNSFATSLYGQSTVDDIFWDIANNCIRIFGFEDCVIYQRDEYREV
                     MIQRAAAGPKNPYQGRDIVNLLEIPIGDGIVGTVAKTSIPELIRNTKRDTRYIVDDER
                     RLSEITVPVMIDGKVYAIIDSEHHRKNYFKKYHLRMLLKIAAISAERISKYLTEERLR
                     TKIARDLHDEMGSTLTSINILSKVGMESGQVSEELRSYLQKIKDN"
     gene            3591..>4072
                     /locus_tag="PLO70_16330"
     CDS             3591..>4072
                     /locus_tag="PLO70_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQZ76088.1"
                     /translation="MYAIVDIETTGSYAAANGITEISIHIFDGTTVVEKFESLINPGQ
                     PIPYYIQAMTGINDKMVAGAPRFEQVAEKIYNLLQDKIFIAHNVNFDYSFIKAHLRES
                     GFELNTKKLCTVRLSRKIFPGFPSYSLGKLCQSLGIPIYDRHRAGGDTAATVKIFQLL
                     "
BASE COUNT         1163 a          922 c          733 g         1254 t
ORIGIN      
        1 gttatctttt attttctgca ggtaggatcg aagttcttca ctcacctgcc cggattccat
       61 tcctaccttg ctcaggatat ttatactggt gagtgtagat cccatttcat catgcaggtc
      121 ccgtgcaatc tttgtacgta atctttcttc tgtcaggtat ttggatatcc gctctgcact
      181 gatagctgca atttttagca gcatccgcag gtgatacttt ttaaaataat ttttccgatg
      241 gtgttcagaa tcgataatgg catacacttt tccgtcaatc atcaccggta cagtaatttc
      301 tgacagcctc ctctcatcat ccacaatata ccgggtatcc ctctttgtat ttcttataag
      361 ttctggtata ctggttttcg ctacggtacc aacgatccca tctccgattg ggatctcaag
      421 caggttgaca atatccctgc cttgataagg gttcttggga cccgctgcag ctctttgtat
      481 cattacttcc cggtactcat ccctctggta aatgacacaa tcctcgaaac caaatatcct
      541 gatacaatta ttggctatat cccaaaatat atcatccact gtggactgcc catataaaga
      601 agtggcaaaa ctattcaact gttcttcttc tttcagtttc tgttgtagtg aatgaatgat
      661 tccaactact gccgccagta ctgccaggat acaaagtacc aaaaaccaac ctgtttgcca
      721 gaaagcagga gatacggaaa ctggtatctc ttttagttct ccggctattc tgccaaacct
      781 gtccacagcc cttgcctgga aagtataagt accggccggc aggttctggt aagagatcct
      841 gaaattatta tcagtccgaa tccaaccggt atcatgccct tttaacctat actcataaag
      901 tatttcatct gaccctccaa agtacaggcc tgcaaactgg agttgcagtg accgggtacc
      961 agaagggatc cttaaagctg acccggtatg taacggtttt aaattctgct gtgctccttc
     1021 cacaaccact tcagttagta tcagcggaaa cgtaatagtg ttttgctccg ggtaaggata
     1081 ataaaacaag acccctcccg cagctgcaag aactaactcc ttcctggctt tatcataaaa
     1141 aatatcatgc gggatattga catccgaacc ataggaagaa aaagtaaagg tcctttcatt
     1201 ggtaaaactg gaaagaggtc ttccggctgt atccatcaca gagataccat ttccggataa
     1261 taaccacagg ttattcttct gatcagccgc aagggatata aaattattgg actccagccc
     1321 cgttcgcata tcaaattttg taaaggcctt cgtataagac tgccccctgt tatacctgaa
     1381 cactccgcct gtatgcgtag ctaaccagat ggtgccgttt tgatcggcag tgatatcatt
     1441 gatgagattt agtgaagcat agtccctggt ttgttctgtt tcataatagg aaaccatttt
     1501 ttttgcaacc aggtcatact tataaagatt tgctcccatg gttccaatcc atacttcttt
     1561 atttatacta tccgaataga gacaagtacc aagcctattg agatatttac ccagtgaatc
     1621 aggtatatca atgcattttt tagctgcggt attataataa tagatacccg cctgaaatcc
     1681 ggcaaaccag agattacctt ctgcatcctg aattacatcc cggaaacctg ccacctctcc
     1741 cttatttggt aacgggaata aactgaaaga tctgcccttg cggtcgtaca gatatacatt
     1801 ccgttccgta agtaaccaaa gcctgttccc tgcatctttc tttacagtaa aacaagctgg
     1861 taaagctgct cccgattggt cctttgttat tcgttcaatc tgtccggttt tacgactaat
     1921 gataaaaaca ctggccgggt tagcagaaca aacaaaatat ttatccactt ccgcatcaaa
     1981 atatacaccg gtcaagtaat ttattccttc ttttccggag gaaaggacca ggggtaaaaa
     2041 ataggaatac aaatgagaat aatcaatact ggtaatacca ttgggagtag ctatccaacg
     2101 gattcctgtt tcatcagtca ttatggaagt cactcctccc ggtaataaag cacccggaaa
     2161 tggaagagtt ggtgatgaaa gaatggcttt atttttaaca ggatcaattt tcagtaatcc
     2221 tttttcagaa gccatccagc tttctgtttg agacaggctc ttaaattcaa tggatggata
     2281 tgcttcaacc tcctgtttca attcaacata atcctttgtt tgttcatcat aactgaatac
     2341 ctggccattc tgaacagaaa gaaatacccg gcgggaaccc ggatcatatt ttatgtcata
     2401 cacatctgca taatacttcc cacccaggaa tgtaaattcc ccggtttcag tttggaggct
     2461 tacgaggcct ttgcttgtac ccagtaaaaa ctcctgctct cctttggcaa caaacttttg
     2521 aatggagtta taattacctg cgttggcaaa ttgcagtttt tccctggcaa aacccggcca
     2581 gtcatagtag gtaaattttt tattctttgt atcataactg gtaaattcaa atacatcaga
     2641 ggcgatccat aatagccctt tattatcaaa ccatatatcc cagattagta aattaggaat
     2701 ttcatttttt gttttcctgg ctcccggcaa taatacattc catgaattat cagccggatc
     2761 ataacaattt aatccgccca tagtagctac ccatattctt ccttcactgt caccggcaat
     2821 atcattgatc acttcattag aaatagaatt ttctttagcg ggtctgaata ttttaaaaga
     2881 atgaccgtca tacctgttca acccgttttt ggttcctacc cacaaaaagc cggaacgatc
     2941 cttataaaaa caggtgaccc ggttatcaga gaggccatcc gttatggtga ggttccggcc
     3001 tttataatga agttgtgcta atactgctgt tcctgcaaaa cagagcagca acaggaatat
     3061 agttcgtatt gagaataaat agttcagtgc ggtaatgtat ttaacgttct aaaatacagt
     3121 ttttatttct tgcctgaaat accattatgg gggataagct ttccctcaac tacaaacagc
     3181 gaagtcattt tggtaatctc ctttaatgaa acggtattga tcttatcttt tcaagtatga
     3241 atttcccgga attagtacta aaaaactatt gccccctttt gaaactcctg atactactgt
     3301 gccaatttcc aggcttttca ctacccagtc ttcatgtgta tcttccgtca tcccctgttg
     3361 tcgtttacac gcagccatat gtaatctttc cctataaaat ttgattcata agggaaactc
     3421 acacagttca tagtcattta ccctgaagca gctaagcccc tgtcgttttc cctgtgtgct
     3481 ggcatgccgg gacaatatgc tcatactgct acagaacagt atcaattttt catccgtgac
     3541 tgatagtgaa attttcacga atttcgtatt taaacctgtg tatttatttc atgtatgcta
     3601 ttgttgatat agaaactacc ggaagttatg ccgctgccaa cggtatcacc gaaatctcta
     3661 ttcatatatt tgatggtaca accgttgtag aaaagtttga gtctcttatc aatcccggac
     3721 agccaattcc atactatatc caggctatga caggcatcaa tgataaaatg gtagccggtg
     3781 ctccaaggtt tgaacaagtg gcagaaaaaa tttacaacct gcttcaggat aagatattca
     3841 ttgcacataa tgtaaacttt gattactcat ttataaaagc tcatttaaga gaatcaggat
     3901 ttgaactgaa caccaaaaag ctttgtaccg tacgattgag cagaaaaatt ttcccaggct
     3961 ttccgtctta cagtttaggt aaactttgcc agtctttggg gataccaatc tatgaccggc
     4021 atcgtgccgg aggcgatact gccgcaaccg taaaaatctt tcagttatta tt
//
LOCUS       DAORTI010000223         4063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28495_length_4063_cov_5.874501, whole genome shotgun sequence.
ACCESSION   DAORTI010000223 DAORTI010000000
VERSION     DAORTI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28495_length_4063_cov_5.874501"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLO70_16335"
     CDS             complement(<1..220)
                     /locus_tag="PLO70_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQZ76089.1"
                     /translation="METNHLQQQLFSHLKENLPAHLSLVDELCDLLDLSADSVYRRIR
                     GEKPITLSELKKICEHYHLSLDQLLQLKT"
     gene            445..1344
                     /locus_tag="PLO70_16340"
     CDS             445..1344
                     /locus_tag="PLO70_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76090.1"
                     /translation="MKQTLITSLILILLNVCASSQQDEINQTDNSFISSESNISTHKK
                     KLNFFVVSKRQKGKLDLATRFNVFRSKIKSLFRPKKFVAIIAKNGEQMSAKVMYRLKK
                     HNARIGTIWFDSHGMYKKGYSLFLIGEDEISYKTLKDSSLASSFRQLAAFSDSETKLV
                     VGSCYGGATYHRASIDYKDTTRMNGDSLMIIIGELLKNVTVYASESWVMTKPGLFLKR
                     AAVTGYPGRKLFLDVCYQPAWENAGKWNEYNAYTNNFRSVTPVTLDMYGNVILRTLSY
                     TDKKEVQKEISKNLEKMQPGLYK"
     gene            1375..1980
                     /locus_tag="PLO70_16345"
     CDS             1375..1980
                     /locus_tag="PLO70_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76091.1"
                     /translation="MKRSLSALLLSLAAMTVFAQKEKYLGVAIFNTQSDLPFGKFSGM
                     FSEIFHPGAEVTWGTNFRIKKKHDWFWDARVAYFFHRFVQHGIPVYANIGYRYKFNKR
                     FAADLALGAGYMHSIPATGKLKLNENGDYENNKGIGRMQANACFGMGAGYLLNPQNVK
                     PVKLFIVYQQRLQMPFVKSYVPILPYNSFMIGFSKSIAGKK"
     gene            2001..3191
                     /locus_tag="PLO70_16350"
     CDS             2001..3191
                     /locus_tag="PLO70_16350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ76092.1"
                     /translation="MKKVIFLHIVLMLMLFSCRKAQITETKSAGVPCPWTDTSNNHPK
                     NSAFTALLEKYKKKGLPGISLLVRDNNGTWIGAAGKADLSNNIDFVPGTVSKAASITK
                     LFVGTLVFKLMEDSIQTGIGYQALNKKISEWLPLSITNKLANGNLITLGQCMKHETGI
                     PDLIEEDPFYLAVLNNPNKKWKAEELLEFIYDKPALFAPSDTAIYSNTNTVLVTMIIE
                     AQTGKKHSDLLKQYVLAPLGLNNTFYQPHDILPISTAQGYYDLYNNGTIVNVSNLVTG
                     SGNGYGGLYSNIYDLYKFINTLLLQRTLLKPSSMTIMETYGKPDDPNQYGYGIMKKFI
                     SRGVNAGYGHSGRDLGYTANMFYFPNKNVTHIFFINYGTDADSDLKEVFNNFQEELLD
                     LTLN"
     gene            3210..3818
                     /locus_tag="PLO70_16355"
     CDS             3210..3818
                     /locus_tag="PLO70_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76093.1"
                     /translation="MKKQIAGLIIIISLFASSCKMAGTLYPISQDQNDFILKKEMIGK
                     WGDSKDTSGYYVVDTFSGKDGKLYQIKTIYKNAEKNTVDTNRFSAILVRLEGWYYLDC
                     WVDMENEFPATDNSYQDWLIPRHFIFRLSFPDANTIEITAPDTEYLIKLIDQKKLLLH
                     YATLKKDDYLILDKPTDLEKGFTESTKYPLLYKDKNVLHRLE"
     gene            complement(3810..>4063)
                     /locus_tag="PLO70_16360"
     CDS             complement(3810..>4063)
                     /locus_tag="PLO70_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQZ76094.1"
                     /translation="DELHLNGIRVFASEMTAEKNVADCDYKTPCAIVMGSEEKGIYPA
                     LMKICDEKIKIPMARDFESLNVSVATGIILYEVMKQRLF"
BASE COUNT         1360 a          767 c          784 g         1152 t
ORIGIN      
        1 ttgtttttag ctgaagcaac tgatcaagcg acaggtggta atgttcacat atttttttta
       61 gttcagaaag agtaatgggc ttttctcccc ggatgcgacg gtatacacta tctgcactta
      121 aatccaacaa atcacataat tcgtcaacca atgatagatg agccgggagg ttttccttca
      181 gatgactgaa aagctgttgc tgcaggtggt tggtttccat gctacaagaa aattacggaa
      241 aatccgtaaa acccaaacta tcattattgt ttttaacaat tattgcctgc aaacaggcaa
      301 tattcaggtg tttctcattc accaattgcg ttaatcaatg cctggtacga aaacctgcca
      361 cagtaacaaa cacggttgct cttttcaatt atcaatcgct tgtttagcgg ggtaggtttg
      421 ggtcacaaaa caaaaacaac agttatgaaa caaaccttaa tcaccagcct aattctgatt
      481 ctgttgaatg tttgtgcatc ttcacaacag gatgagatca atcaaaccga taactcgttc
      541 atcagttctg agtcaaacat ttcaacgcat aaaaagaaac ttaatttttt cgtagtttct
      601 aaaagacaaa aagggaaact tgatcttgcc accaggttta atgtgtttcg atccaaaata
      661 aaaagtcttt tcaggcccaa aaagtttgta gccataatag caaaaaacgg tgaacaaatg
      721 agtgcaaaag tgatgtaccg tcttaaaaaa cataatgccc ggatcgggac catctggttt
      781 gattcacacg gtatgtataa aaaaggctat tctcttttct taattggaga agatgaaatc
      841 agttacaaaa cgctgaaaga ctcatcttta gcctcttcat ttcgccaatt ggctgctttt
      901 tcagacagtg aaacaaaact tgttgttggt tcctgctatg gaggtgccac ttaccaccgg
      961 gcctcaatag actacaagga tactacccgg atgaatggcg attctttgat gataattatc
     1021 ggggagttat taaaaaatgt aactgtctat gccagcgaga gctgggttat gacaaaacca
     1081 ggtctttttc ttaagagagc agcagtaaca gggtaccccg gacgtaaact ttttcttgat
     1141 gtttgctatc agccggcctg ggaaaatgct gggaaatgga acgaatacaa tgcgtatacc
     1201 aataacttca ggagtgtaac gccggttaca ctagatatgt atggaaatgt tatcctgcgg
     1261 accttatctt atacggataa aaaagaagtt cagaaagaaa tttccaaaaa tctggagaaa
     1321 atgcaaccgg gcctttataa atagtataga cctttacatt caaatacgaa agcaatgaaa
     1381 cgatcattat ctgcactatt gctgtcgtta gcagcgatga cagtatttgc acaaaaagaa
     1441 aaatacctgg gagtggcaat atttaatacc cagtcagatc tcccttttgg aaaatttagc
     1501 ggtatgttct cagaaatatt tcatcccgga gcagaagtta cctggggaac aaatttccgg
     1561 ataaagaaaa agcatgattg gttttgggat gcaagggtag cttatttttt tcaccggttt
     1621 gtgcaacacg gtattcctgt ttatgccaat attggttacc gctataaatt caataagcga
     1681 tttgcagctg accttgcatt aggtgctggc tacatgcatt ctatacctgc taccggtaaa
     1741 ttgaaactga atgaaaatgg tgactatgag aataataaag gcattggcag aatgcaggct
     1801 aatgcctgct ttggaatggg ggccggatac ctgcttaatc ctcaaaacgt gaagccggta
     1861 aaattgttta ttgtttatca gcaacggtta caaatgccat ttgtaaaatc atatgtacct
     1921 attcttccct ataacagttt tatgatcggg ttcagcaaat caatagctgg aaaaaaataa
     1981 ctaacctaaa tgaaagcaac atgaaaaagg taatattcct tcatatagtt ttaatgctga
     2041 tgcttttttc ttgccggaaa gcacagatca cagaaaccaa aagtgctggt gtaccctgtc
     2101 catggacgga taccagtaat aatcatccta aaaattcagc atttactgca ttacttgaaa
     2161 aatacaaaaa gaaaggactg cccggaatat ctctgctggt tcgggataat aacggcacct
     2221 ggataggcgc tgccgggaaa gcagatctgt caaataatat tgattttgtg cccggtactg
     2281 tctcaaaagc tgcgagtata acaaaattat ttgtgggaac tcttgttttt aaattgatgg
     2341 aagattccat tcaaactggc ataggatacc aggcattaaa caaaaagata agtgaatggc
     2401 tgccattatc aattactaat aaattagcca acggaaattt aattacactg ggtcagtgta
     2461 tgaaacatga aacaggcatc ccggatctga tcgaagaaga cccgttctac ctggcagtgc
     2521 tgaataatcc caataaaaaa tggaaggcgg aagagttact ggaatttatt tatgataaac
     2581 ctgcattgtt tgcaccctca gatacggcca tttactcaaa caccaatacc gtcctggtaa
     2641 caatgataat tgaagcacaa acaggaaaaa aacattcaga ccttttaaaa cagtatgtat
     2701 tggcaccgtt aggattaaac aatacatttt atcagccgca tgacatactg ccaatcagta
     2761 cagcacaggg ctattatgat ttgtataata acggaactat tgttaatgtg tctaatctgg
     2821 taacgggaag cggaaatggt tatggtggat tatacagcaa tatctatgac ctctataaat
     2881 ttatcaatac cttgttgctc caaagaacat tactaaagcc ctcctccatg actattatgg
     2941 aaacatatgg caaaccggat gaccctaatc aatacggcta cgggattatg aaaaaattta
     3001 tcagcagagg tgtaaatgcc gggtatggcc atagcggcag agacttaggc tacacggcaa
     3061 atatgtttta tttccccaac aagaatgtaa cacatatttt ctttatcaat tatggaacag
     3121 atgcagacag tgacctgaaa gaagttttca ataatttcca ggaggagtta ctggacctga
     3181 cgcttaatta aaaataaaac ctttataaaa tgaaaaagca aatagctggg ctcattataa
     3241 tcatttcttt atttgccagt tcctgtaaaa tggcaggcac tttataccca atatctcagg
     3301 atcagaatga ttttatccta aaaaaagaaa tgatcggaaa atggggagac agtaaagata
     3361 cctcgggcta ttatgttgta gacactttca gcggtaaaga cggcaagcta tatcagataa
     3421 aaactattta taaaaatgca gaaaaaaata cagtggatac taacaggttc agtgccatcc
     3481 ttgtcagact agaaggatgg tattatcttg actgctgggt agatatggag aatgaatttc
     3541 ctgcaactga taacagctat caagattggt taataccccg gcatttcatt ttcaggctat
     3601 cattcccgga tgcaaacaca atagaaataa ccgctcctga tacagaatat ctgataaagc
     3661 taattgatca aaaaaaactc cttttgcatt acgcaacgct gaaaaaggat gattatttaa
     3721 tacttgacaa gcctactgat ctagaaaaag ggtttaccga atcaacaaaa tatccgttgt
     3781 tatataagga taaaaatgtc ctgcacaggt tagaataatc gttgcttcat tacctcatac
     3841 aaaataatcc cggtggcaac ggaaacgttg agtgattcaa aatccctggc catcgggatc
     3901 ttaattttct catcacaaat tttcattaat gcgggataga tacctttttc ttcacttccc
     3961 attacaatgg cacatggtgt tttataatcg cagtcagcta cattcttttc agctgtcatt
     4021 tcgctggcaa atactctgat gccattcagg tgcaactcat caa
//
LOCUS       DAORTI010000224         4030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_28859_length_4030_cov_5.343899, whole genome shotgun sequence.
ACCESSION   DAORTI010000224 DAORTI010000000
VERSION     DAORTI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28859_length_4030_cov_5.343899"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLO70_16365"
     CDS             <1..441
                     /locus_tag="PLO70_16365"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQZ76095.1"
                     /translation="GDEGLIGNEEEMIIKPAIKEAKNNNLLVIGPYSADAFFARRSFE
                     KFDAVLAMYHDQGLIPFKTLATGEGVNYTAGLPSIRTSPDHGVAFDIAGKDKADTSSF
                     LTALFECIDIFNRRNGYEESRKNPLRKMTPAILANAKDEIIEEG"
     gene            complement(445..1134)
                     /locus_tag="PLO70_16370"
     CDS             complement(445..1134)
                     /locus_tag="PLO70_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76096.1"
                     /translation="MKNILFLIFFMCGAFVAKTQTAEEVINNVKAKLEKVNDYEASGK
                     MKTNVIFIKAPIANVKVYYKKPNKLRINNENGISFIPKGSVNINLNNIFINTNGFDII
                     DVGKESGTNLRIIKLLPKDDNAEVVLSTLYIDETLSLIKKAKTTTKENGTYELEMTYG
                     KYAAYGLADKVNFSFNTKDYKLPKGITFDYDDGSKKTEPADKMKNKKGKVEIVYSGYS
                     INKGVPDSVFN"
     gene            complement(1146..>1745)
                     /locus_tag="PLO70_16375"
     CDS             complement(1146..>1745)
                     /locus_tag="PLO70_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQZ76097.1"
                     /translation="EANMGTLRLSLTKPVSRTEYMLVKFAASVVYTLALLVWMAALAL
                     FGSMLLFGTNDLVVLRSEGIEQMKSFDVLWRYFAAFGYAAVALTTVSALAFLLSVFAE
                     NSIGPIIATMSIVIVFTILSEMNIPIYDTTVKPYLFTSHMVAWKGFFYVKADAEGTTI
                     NGSIENLPAVLKSLGILVAYIILFFSTAVWVFRKKDILT"
     assembly_gap    1746..1845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1873..2802)
                     /locus_tag="PLO70_16380"
     CDS             complement(1873..2802)
                     /locus_tag="PLO70_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76098.1"
                     /translation="MVPIIEVKHLTKYFGDLKAVNDLSFTVQEGQVYGFLGQNGAGKS
                     TTIQMLLTLIKPTSGNIEIFGLDLQKHRKEILRQVGAIIERPDLYKYLTALENLRIFA
                     AMSGIQISEKKLMDQLAMVGLEQRAHSKVKTYSQGMKQRLGIAIALVHDPKLIILDEP
                     TNGLDPQGIADIRNLILMLSRDMHKTLLISSHLLSEMELIADSMIIIDKGKKLVEGKV
                     NELFDPAETIVELKTTDDSETYNKLLQSSFKGFVQGKRNDCVLMKLHRNKVPEVMKEL
                     VRMDVNVISLHSKHSLEDYFLSLTTGQSNVEIA"
     gene            3113..3526
                     /locus_tag="PLO70_16385"
     CDS             3113..3526
                     /locus_tag="PLO70_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76099.1"
                     /translation="MTKYLILAIVLTWKLPDVHWSNQNDYAPFTSLSFQSHKEPKFPG
                     RVISLDGTINNNKVFLNWKVEENEAVYMFEVEKSLDGKNFTTAAIVFGTDKSETEEYQ
                     FFEKASKPKSVYRIKLINKQRQVAYSKIITVSPAS"
     gene            3540..3683
                     /locus_tag="PLO70_16390"
     CDS             3540..3683
                     /locus_tag="PLO70_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76100.1"
                     /translation="MSEKINKSLKQEYNRLKKAVEKILNVKKQQTPQLILQPIRNNNR
                     VSH"
     gene            complement(3752..>4030)
                     /locus_tag="PLO70_16395"
     CDS             complement(3752..>4030)
                     /locus_tag="PLO70_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQZ76101.1"
                     /translation="DPILHSQLRLAVMSLLISVKEAEFTFIKEKTNSTAGNLSVQIQK
                     LKDAGYIDVTKQFKDNYPQTSCRITKQGIKAFEEYVTNLQSYLGKNGK"
BASE COUNT         1235 a          819 c          681 g         1195 t
ORIGIN      
        1 ggtgatgaag gcctgattgg aaatgaagaa gaaatgatca ttaagcctgc cattaaggaa
       61 gccaagaata ataatttgct cgttatcgga ccttatagtg ccgatgcttt ctttgcccga
      121 cgcagttttg aaaaatttga cgcggtattg gccatgtacc atgaccaggg actaattccg
      181 tttaagacac tggctaccgg tgaaggtgta aactacactg ccggattgcc atccattaga
      241 acctctccgg accacggagt ggcatttgat attgccggca aggacaaagc agatacttcg
      301 tctttcctga ctgccctttt tgaatgtatt gatattttta accgcagaaa cggctatgaa
      361 gaaagccgga aaaacccatt acggaaaatg acaccggcta tattggcaaa tgccaaagat
      421 gagataattg aagaaggcta aactttagtt gaaaacagaa tcaggtacgc ctttattaat
      481 actgtaaccg gaatacacaa tttcaacctt acccttttta tttttcattt tatctgctgg
      541 ttcagttttt ttagatccat catcatagtc aaacgtaatg cccttgggta atttataatc
      601 ttttgtgttg aaagaaaaat tcactttatc ggccaggcca tacgctgcat acttaccgta
      661 tgtcatctcc agttcataag tgccgttttc ctttgtcgtt gtctttgcct ttttaatcaa
      721 tgaaagcgtt tcgtcaatat acaatgtaga caaaaccacc tctgcattat catctttggg
      781 tagcaattta atgatacgga gattcgtacc cgattctttt cccacatcga ttatgtcaaa
      841 cccgttggta ttgataaaaa tattattcag gttgatattc accgatcctt tgggaataaa
      901 ggaaatacca ttttcattat ttatcctgag cttattgggt tttttataat acactttgac
      961 attagcaatc ggagccttaa taaaaatcac attggttttc attttaccgc tggcctcata
     1021 atcattcact ttttcgagtt ttgccttcac attattaata acctcttcgg ctgtctgtgt
     1081 ttttgccaca aatgccccac acataaaaaa gataagaaaa agaatatttt tcattctgcc
     1141 aggatttaag tgagtatatc tttttttctg aacacccata cagccgtact aaagaatagg
     1201 ataatataag cgaccaatat tccaaggctt tttaatacag ccggtaaatt ttcaatactg
     1261 ccattaatgg tagtaccctc cgcatccgcc tttacataaa aaaaaccttt ccatgctacc
     1321 atgtgcgatg taaacaaata gggttttacc gttgtatcat agatcggaat attcatttca
     1381 gataaaatag tgaaaacaat cacgatactc atggttgcaa ttatcggacc gatagaattt
     1441 tcagcgaata cacttaatag aaaagccagc gccgaaacgg tagtcagtgc tactgccgca
     1501 taaccaaagg ctgcaaaata ccgccataac acatcaaagc ttttcatttg ttctatcccc
     1561 tccgatctaa gcaccaccag gtcattggtt ccgaatagga gcatacttcc aaacaaagcc
     1621 agcgctgcca tccaaaccag taatgccaat gtatatacta ctgaagctgc aaatttcacc
     1681 agcatatatt cggtacggct taccggcttg gttaaggata gccttaatgt tcccatattt
     1741 gcttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctttt gaatatttta
     1861 aacaactcta ttttaagcaa tttcaacatt tgactgcccg gttgttaatg ataaaaaata
     1921 atcttcaagt gagtgtttgg aatgcaatga aatgacattt acatccatcc tgaccagctc
     1981 tttcatcact tccggcacct tattccggtg caacttcatc agcacacaat cattcctttt
     2041 gccctgtaca aatcccttaa aagaagattg cagtagttta ttgtatgttt cgctatcgtc
     2101 agttgttttc aattcaacaa tagtttccgc agggtcaaac aattcattta cttttccttc
     2161 cactaatttc tttcctttat cgattatgat catagaatca gcaatcagtt ccatctcact
     2221 taataagtgt gacgagatca ataaggtctt atgcatatcg cggctgagca taagaataag
     2281 attccggata tcagcaatcc cctgcggatc gaggccatta gttggttcat caaggatgat
     2341 aagtttgggg tcatgcacca atgctatcgc aattcccaac cgctgtttca tcccctgcga
     2401 atatgttttt actttgctgt gtgcccgttg ttccaatccc accatcgcca gttgatccat
     2461 taatttcttt tcagaaattt ggatgccgct catcgcagca aaaatgcgca ggttctccaa
     2521 tgccgtcaga tatttgtaga gatcgggtcg ctcaatgatt gcaccaacct gcctgaggat
     2581 ttctttccgg tgcttttgca aatccaggcc aaagatttcg atatttcccg atgttggctt
     2641 gataagtgta agcagcattt gtatggtagt ggatttaccg gctccgtttt gtcccaaaaa
     2701 tccatatacc tggccttcct gtacagtaaa agaaagatca ttgactgctt ttaaatctcc
     2761 gaaatacttg gtaagatgtt ttacttcgat aattggtacc atcactgaca gataacgggc
     2821 aaaggtatta aaggttcacc cctgctaatg aagtatttaa gagtttaaga aaatatttct
     2881 taccaaagac ttgcaaatgt tacgttcata gattaggttt gttttcgatt cggaaaacca
     2941 catttaccca cgtaaaacta ctataaacat ccgtaccaaa actcactgtc ctctccacca
     3001 attgggtacg tatcttaaag tagaagcaac attgggataa aataacaacc gaaaactaac
     3061 atccaatatt tctgtgggcc atttgaagat caaattcaaa aaaccctaag aaatgacaaa
     3121 gtaccttatt ctggcaatcg tattaacctg gaagctacca gatgtgcatt ggagcaatca
     3181 aaatgactat gcccctttta caagcctttc ttttcaaagt cataaagaac caaaatttcc
     3241 gggcagggta atcagcctgg atggcacaat taacaataat aaagtgtttt taaactggaa
     3301 agtagaagaa aatgaagcag tttacatgtt tgaagtagaa aaaagcctgg atggtaaaaa
     3361 ttttacgacc gcagcaattg tttttggtac tgataaatcc gaaactgaag aataccagtt
     3421 ttttgaaaaa gccagcaaac caaaatcagt gtacagaatt aagttgataa ataaacaacg
     3481 ccaggtagcc tattcaaaaa taataactgt aagcccggcc agttaaataa ataaaaaaca
     3541 tgtcagaaaa aataaacaag tcgctgaagc aagaatataa ccgtttgaaa aaagcggttg
     3601 aaaaaatatt aaatgtaaaa aagcaacaaa ctccccagct tattttgcaa cccataagaa
     3661 ataataacag ggtaagtcat taaccaaccc tcaccttttt catacactta gagtatcaaa
     3721 agagaaaaag ccctttgtct aaagggcttt ctcattttcc gtttttacca agataagatt
     3781 gcaggttcgt aacatactct tcaaaagcct tgatcccctg ctttgttatt ctacacgatg
     3841 tttgggggta gttatcttta aattgctttg tcacatctat ataaccggca tcttttaatt
     3901 tttgtatctg cacactaagg ttaccggcag ttgagtttgt tttctcctta ataaaggtaa
     3961 attctgcctc tttcacacta ataagcagtg acataacagc taagcgaagc tgcgagtgca
     4021 atatagggtc
//
LOCUS       DAORTI010000225         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_29396_length_3978_cov_10.381596, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000225 DAORTI010000000
VERSION     DAORTI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29396_length_3978_cov_10.381596"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLO70_16400"
     CDS             complement(<1..582)
                     /locus_tag="PLO70_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQZ76102.1"
                     /translation="MKCHRLSLLIIPLLSFIPGWSQQRMIPDEIDGKFFNPSTLLWYT
                     GPAKKWDEALPVGNGRLGAMVFGKNGEERIQLNEETYWSGGPYSTVVKGGYKVLPKIQ
                     KLVFEEKYLAAHNLFGRNLMGYPVEQMKYQSLANLHLFFKNQDSIANYKRWLNLEDGV
                     AGVTYSANGVTYQRQVFASAPDQVIVVRITADKP"
     gene            1184..2467
                     /locus_tag="PLO70_16405"
     CDS             1184..2467
                     /locus_tag="PLO70_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HQZ76103.1"
                     /translation="MKHFISYCFFLFLLPAFVAAQTVVSIKVEGAINPVTASFIHDGI
                     QKAAAQKAECLLIHLNTPGGLLKSTRLIVSDILESPVPVIVYVSPAGAQSGSAGVFIT
                     LAAHIAAMAPGTNIGAAHPVSLQGPMDSIMGEKVTNDAAAFIRSIAEKRKRNMEWAEN
                     SVRKSFAYSETEALDYEAIDLIATNVPDLLNQLEGKIISLQQGTVTLHTKAAKVEVYS
                     MNFFQQFLHIISDPSIAYILLLLGLFGILLEFFNPGAIVPGVVGGISLILAYYAMHTL
                     PINFAGLALILFALILFLLEIKIVSHGVLAIGGVVSLLLGSLMLIKPGSALELAAISR
                     PVIIAATAVASLLFLFIIGAGIKAQRHKVVTGLEALIGDTGEVITILSPTGTVQVQGE
                     IWNAESMAGTINPGEKVRIKEMKNLKLYVEPIAHT"
     gene            2477..3256
                     /locus_tag="PLO70_16410"
     CDS             2477..3256
                     /locus_tag="PLO70_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQZ76104.1"
                     /translation="MQQFPYLVAGIIFFILIILASAIRILREYERGVIFRLGRLIGNG
                     GVKGPGLILLIPFIDRMVKVSLRTVVLDVPPQDVITQDNVSIKVNAVVYFRVMEPQKA
                     VVQVENFLAATSQISQTTLRSVLGQSELDDLLSQRDKINHKLQQIIDTNTEPWGVKVS
                     NVEVKQIDLPQEMQRAMAKQAEAERERRSKVIAAEGEFQASQRLADAAKILGEQPSAL
                     TLRYLQTLREIATENNSTTIFPVPIDLLKPFLNSGGKKLES"
     gene            3605..>3978
                     /locus_tag="PLO70_16415"
     CDS             3605..>3978
                     /locus_tag="PLO70_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76105.1"
                     /translation="MKRIFTLFIALGLITAVQAQNGRGDNRDGRQRDQQTERQFDQRD
                     FDNGYSNDQYDRDVRYVKTNPGMQRKIAKQVYAINREYDFKIDRVKNNFFLSRWEKQR
                     QVRFLQEQRQREIRMVYARFHK"
BASE COUNT         1105 a          883 c          905 g         1085 t
ORIGIN      
        1 gggcttatca gcagtaatgc gtactacaat tacctggtcg ggtgcggaag caaatacttg
       61 ccgttgataa gtaacaccat tagcggaata agtaacacca gcaaccccat cttcaagatt
      121 cagccatctt ttataattgg cgatgctgtc ctggttttta aaaaacaaat gaagattggc
      181 aaggctctgg tatttcattt gttcaaccgg gtagcccatt aaatttcttc cgaataaatt
      241 atgggcagct aaatattttt cttcaaatac taatttttga attttgggca atactttata
      301 gccgcctttc accaccgtag aataaggacc acccgaccaa tacgtttctt catttaattg
      361 aatgcgttct tcgccgtttt ttccaaagac catggcgcca aggcggccat tacccacagg
      421 aagggcttcg tcccattttt ttgccggccc ggtataccat aaaagggtgg aaggattaaa
      481 aaatttgcca tcaatttcat caggtatcat ccgctgctgc gaccaaccag ggatgaatga
      541 taataaaggg atgatcagca atgataatct gtgacatttc ataagcagtc agcaattaat
      601 gtaagcggtt ttaaaaatag tatctgcaaa gggcgggttc gtgattaaat ataaagcttt
      661 tgtcactatt gcttcttagc gtaatgcagg aaaataatca atcaataaag aagaaaatat
      721 tcccgctatt tcattttgag taatgagtag ctaaagcctt ttgtatgtaa gagtttccga
      781 atcaacattt ctggttttgt tttgctgctt cgtatctgcc acatgttata gcttcttgtt
      841 tttttattaa gcatcccgat ttattttggg aaagattccg ggtcaagccc ggaatgacag
      901 ccggaactac attacgtagg ggcctcgatg acagccggaa tgacatctcg ttggaccggg
      961 atgatattac tataaaagct ctggtttggt atcatgcatt ccgatttaca tcgggacagg
     1021 ctatcggcca tagaattaag ttactaaggg aaagattccg ggtcaagccc ggaatgacat
     1081 catgtgagct atccaactaa tctccaccgt tgtacccaaa atgaagtaaa ttacaggtgg
     1141 ctcattgcta atcccgggcc ggctcccgtt ggggaggctt cttatgaagc attttatatc
     1201 atattgtttc tttctcttcc tgctccccgc ttttgttgcc gcacaaaccg tggtttcaat
     1261 aaaagtagag ggtgctatca acccggtcac cgcttctttt attcatgatg gcatacaaaa
     1321 agcagccgct caaaaagcag aatgcctgct tattcatctt aatactcccg gtggtttgct
     1381 gaaatcaacc cgcctcatcg tcagcgatat actggaatcg cctgtgccgg tcattgtata
     1441 tgtgtcgcca gccggggcac agtcgggctc agccggtgta ttcattaccc tggcagcgca
     1501 tattgccgcc atggcgccgg gtaccaatat cggggctgcc cacccggtaa gcctgcaggg
     1561 accgatggac agcatcatgg gcgaaaaagt aaccaatgat gcagcagcct ttatccgttc
     1621 catcgccgaa aaaagaaaac gcaatatgga atgggccgaa aattctgtcc gcaaaagttt
     1681 tgcttattca gaaaccgaag ccctcgacta tgaagccatt gatctcattg ccaccaatgt
     1741 gcccgacctg ctgaaccagt tggagggcaa gatcatttcc ctgcagcagg gcacggtaac
     1801 cctgcatacc aaagcagcga aggtggaagt atacagcatg aatttcttcc agcaattcct
     1861 gcatatcatc agcgatccca gcatcgctta tatcctgttg ttgctgggtt tgtttggtat
     1921 cctgttagaa ttcttcaatc ccggcgccat tgttcccggt gtggtgggag gcatcagtct
     1981 tatacttgca tattatgcca tgcatacttt acccatcaac tttgccgggc tggccctcat
     2041 cctttttgca ttgatacttt ttttactgga aataaaaatt gtcagtcatg gtgtactggc
     2101 catcggcggc gttgtttcct tactgttagg ttccctgatg ctgataaagc cgggctcggc
     2161 cctggagctg gctgctattt cccgacctgt tattatcgct gccactgcgg tggcttccct
     2221 gttgttctta ttcatcatcg gggcgggcat taaagcacaa cgccataaag tggttaccgg
     2281 actggaagca ttaattggcg atactggtga agtgatcact atactatcac ccaccggcac
     2341 ggtacaggta cagggagaaa tatggaatgc ggaatccatg gccggtacga tcaaccccgg
     2401 agaaaaagta cggatcaaag aaatgaagaa cctgaaacta tatgtagaac ccattgctca
     2461 cacttaaaaa aattttatgc aacaatttcc ttacctggta gccggtatca tcttttttat
     2521 actgatcatt ttggccagtg ccatccgtat tctgcgggaa tacgaacggg gtgttatttt
     2581 ccggctgggc cgcctcattg gtaatggtgg cgtgaaaggt ccgggcctca tcctcttgat
     2641 tccctttatc gacaggatgg taaaagtaag cctgcgtacg gtggtactgg atgtgccgcc
     2701 gcaggatgtg atcacacagg acaatgtatc cattaaagta aatgcagtgg tttatttccg
     2761 ggtgatggaa ccgcagaaag cagtggtaca ggtagagaat tttctcgctg ccacttcgca
     2821 gatctcacaa actacgttgc ggagtgtgct gggacaatca gaactggacg acctgttgtc
     2881 acaacgggat aagatcaatc acaagctgca gcagatcatt gataccaata cagaaccgtg
     2941 gggcgtaaaa gtgtctaacg tagaagtgaa acagatagac ctgccgcagg aaatgcaacg
     3001 ggccatggcc aaacaagcgg aagcagaacg ggaacgccgt agtaaagtga tcgctgccga
     3061 aggagaattc caggcatcac aacgattggc cgatgccgcc aagatattgg gtgagcaacc
     3121 cagtgcttta acgctgcgct acctgcagac cttacgggaa atagcaaccg aaaacaattc
     3181 cactactatt ttcccggttc ccattgatct gctgaaacct tttttgaaca gcggaggaaa
     3241 aaaactggaa tcatagataa ttttttattg cagtaataga aatgaaattc ccgaagtctg
     3301 tcagcctgag cttgtcgaag gcgattggtt tgattgccaa tgtttcgata aactcaacat
     3361 gacaatcaaa cccatttttt gtgtcagcct gagcttgccg aaggccagtt tatacaacac
     3421 acttcatcat cattttttta ttaaaaactt gcggcaacaa ctgttaattg gccaaaaata
     3481 aaaaggttac tggttgttct atgtagtaag tacatagtga tgagtggagt attaacgttc
     3541 tgtttgtaaa cagtggttct tcgtttgcat cctatccatt attattcatt taaaacaatt
     3601 cattatgaaa agaatcttta ctctgtttat tgcactggga cttatcaccg ccgtccaggc
     3661 acaaaacggc cgcggtgata accgtgatgg ccgtcaacgg gatcagcaaa cagaaagaca
     3721 atttgatcag cgggattttg ataacgggta cagtaatgac cagtatgaca gggatgtccg
     3781 ctatgtaaaa acaaatcctg gtatgcaacg taagatagcc aaacaggtgt acgctatcaa
     3841 ccgggaatat gatttcaaaa ttgatcgggt aaaaaacaat ttcttcctga gccgttggga
     3901 gaaacaaagg caggtacgtt ttttacagga gcaacgtcag cgggagatca ggatggtgta
     3961 tgcccgtttt cacaagaa
//
LOCUS       DAORTI010000226         3840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_31085_length_3840_cov_4.272391, whole genome shotgun sequence.
ACCESSION   DAORTI010000226 DAORTI010000000
VERSION     DAORTI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31085_length_3840_cov_4.272391"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2042..2560
                     /locus_tag="PLO70_16420"
                     /pseudo
     CDS             2042..2560
                     /locus_tag="PLO70_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            2818..3288
                     /locus_tag="PLO70_16425"
     CDS             2818..3288
                     /locus_tag="PLO70_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76106.1"
                     /translation="MTRTIISFLLLTLYLQGIAQIPSTKETIQSFIDSIGKSNDKQLI
                     ESSIFPAFANGTNFKWRQKIWRNNKKELLWVETIIPDSISTVYFYCHDTLIFASERTY
                     ITDSISNKRNSIFRNIYILESRIIEDSAPGRNNNTIEHYLQESKKYLEHAKLAD"
     gene            3529..>3840
                     /locus_tag="PLO70_16430"
     CDS             3529..>3840
                     /locus_tag="PLO70_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76107.1"
                     /translation="MKTSIDIASFFIVLQALVGITGGIIYINIPEQATKSLNDSIIDF
                     YLHLNGIFYGATLFFGLICGFILKRTNRLIISALLSLAVGLGFLLLHALVLPVPLFAF
                     LS"
BASE COUNT         1213 a          799 c          814 g         1014 t
ORIGIN      
        1 caacaggctg ccggatagct ggaaatagcc tggtgcacac tccaaacaat ttacaatacc
       61 ccgatggcag caagcttttt tggatgtaac ttgttggctt aaaattattt ttatggtttt
      121 gaaaagatac tttctttcgt tcctgttttt tttatccctc acaatagtac atgcacagga
      181 tgatggtggt tataaaacac ccccgaaaga tgtggcagat atgctactcg ctaaaagacc
      241 tcccaatgta agtgtggatg ataatgcaga gtggatgctt tttatggagg gcagcagtta
      301 tccatccgtg gaagaactgg cccggcctga attaaaaatt gcaggccttc gtatcaaccc
      361 cgccaatttt gcgcccagcc gccagaattt tgttaccaat ctttccctga agaatatcaa
      421 gaccaacaaa gaatataaga tcaccggcct gcccgttccg ttatatgcag gcagtatcag
      481 ttggagcggt aataataaaa agatcgcttt cacccatacc actgccagtc gtgttgatct
      541 ttatgttatt gatgtggcta cccaaaaagc catgaaggtg aataaaacgg cgttgaacac
      601 tatcaccggt ggctaccagt ggtatgatga taatacattg ctttaccgta ctattttaaa
      661 accagctgcc gccgcaccgc ccaaaccggc cgtaccaacc ggaccgacca tccaggaaaa
      721 ttatggcaaa gcttctcccc gccctacttt ccaggacctt atcaaaagtc cttatgatga
      781 aacactgtat gccttttatg ccaccacgca actgatgaag aacagcaacg gagtggaaac
      841 aaaaattgga cagcctgcta tttatagcat gatcagtgta tcgccggata aaaaatatat
      901 gctgctccgc acattgaaaa aaccattttc ctatactgtt ccggccggcg gtttcccttc
      961 tatcgtagcc attacagata tgaatggaaa agttttaaaa gaattagcag acctgccatc
     1021 agcagaaaca gcacccgctg gttacgataa tgtgcaactg gtacccaggg cttttgaatg
     1081 gagagatgat gaaccggcca ctgccgtgtg gtgtatgccg ttggacagtg gatttatcaa
     1141 aaagaatgta gaataccgcg atgcggtcta tgccttgtca gctcctttta aaggcatgtc
     1201 caaactgctt tttaaaacca aaatgcgtta tagcggcacg gtatggggcg atgccaacct
     1261 ggctatcgtt actgaaggat taagaagcaa acaggtggta caaatggatc gttttaaccc
     1321 cgtaacagaa gacctggaaa aactgatgac aagaaatacc acggatgcat attctaatcc
     1381 cggtttcccg gttaccaaac ccaaccagta tggaaaagaa gtattgtgga ccattgataa
     1441 cggcaataaa atattattga acaacagcac cggcagttcg cccaaaggcg atctaccttt
     1501 cttattgtcg tatgatgtgc ataccaaaaa agcagataca ctctggcgct gccaggaagg
     1561 ttattttgaa aatgtagtga aagtgctgga tgcagacaaa ttgcaactgc tgacctcgaa
     1621 agaaagtgaa aaagaagtac ccaactactg gatcaaaaat ttaaaattgc ggatagcgga
     1681 caggcaactc acacaattca gcaatcctta cccgcaaatg gtgggagtaa gccaggaaaa
     1741 aataaaatac aaaagagcag atggagtgga cctgaccggt gacctgtttt tacccaaagg
     1801 ctatgatccc aaacgggatg gcaaattacc ggtgatcatc tgggcatacc cgagagaatt
     1861 taattctgcg gctgatgccg cacaggtacg tggcagtgag catcgtttta cattagtggg
     1921 cggaggctcc ccggtgtttt atgtaacaca gggatatgca gtactcaaca atgcagaaat
     1981 gcccatcgtg gcaaccaata aagatgctaa acccaatgat aatttcattg agcagttaac
     2041 gatgaatgca gaagctgcca tcaatgtgct cgattcaatg ggtgtgggtg ataagaaccg
     2101 ggtggccgta ggcgggcata gctacggtgc atttatgact gccaatttat tagcgcatac
     2161 caaactcttt aaagcaggta ttgcaagaag cggtgcttat aaccgctccc tcactccttt
     2221 tggtttttag aatgaggacc gtacgtactg gcaggcgcag gacctgtata caaagatgag
     2281 tgctttcagt aatgccgata aaataaaaac accgatcctg ctgatacatg gagaaatgga
     2341 taataacacc ggcactttcc cgatacagag tgaaagaatg tacaatgcca ttaaaggaca
     2401 tggcggcacg gtaaaatatg taagcctgcc gtatgaaagc cacggctatg cggggaggga
     2461 aaatgtattg cataccctgg cagaacagtt tgaatggctg gagaaatatg tgaagaatgc
     2521 ggaaaagaaa accgagaagg aagagaagaa agggttctga gcctgtcgaa gaatctgccc
     2581 caaaaaaatt gtgtatgaaa atcccgggcc aatgccggga ttttttactt actgcacagt
     2641 tgaattgtcg caactaaaat ggggtgaaaa aaatctgcat aaatcagctt aaatcaatat
     2701 aaatcaaagc aacaatacaa tgcttataaa aatatagaag gttcgtatat tcgacaatac
     2761 atactttgtg cgcaatcatc aacgacattg gaagtagatt ttttttaata atgacctatg
     2821 acaagaacaa tcatttcgtt tcttttgctg acattgtatt tacaggggat tgcacaaatt
     2881 ccttctacaa aagaaacaat tcagagtttt attgactcca ttggtaagag caacgataaa
     2941 caactaatag aaagttccat ctttcctgca tttgcgaacg gcacaaattt caagtggagg
     3001 caaaaaatat ggcgcaataa taaaaaggaa ctgttatggg tagaaaccat tataccagac
     3061 tcgatttcca ccgtgtattt ctattgtcat gacactctta tttttgcctc tgaacgtact
     3121 tatattactg atagcatttc aaacaaacgc aatagcattt ttagaaatat ttacatcctt
     3181 gaatctagaa taattgaaga ttcagcgcct ggaagaaata ataacacaat agagcattac
     3241 cttcaagaaa gcaagaaata tcttgagcat gctaaactag ctgattaaaa aattacggtc
     3301 aaatgcgcac aacattcagt tttgcgtcag caggggacga cgaatatatc ctcatcggca
     3361 gaactactat cagctacata tccggctgac cgaacacaga tgagcaacag aatatatttt
     3421 caacttttgt aacttcgttc ggcgcagtac cgggctggac gttttccaaa tgccctgccg
     3481 aacgcaaagc cctgctcgtt agctgccatt ttgcaagaac attttagttt gaaaacttca
     3541 atagacatag catcgttttt tatagtgcta caggcactgg tgggaattac gggaggcatt
     3601 atttacatta atattccaga acaagcaact aagtcgctaa atgactctat cattgacttc
     3661 tatctgcatt taaatggtat attctatgga gcaactcttt tctttggttt gatttgcgga
     3721 tttattttga aacgtactaa cagattaatt atctcggcct tactgtcttt ggcagttggg
     3781 ttaggctttt tattgttgca tgcactagta ttaccagtac ctctatttgc ttttctttct
//
LOCUS       DAORTI010000227         3819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_31368_length_3819_cov_7.067481, whole genome shotgun sequence.
ACCESSION   DAORTI010000227 DAORTI010000000
VERSION     DAORTI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31368_length_3819_cov_7.067481"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLO70_16435"
     CDS             complement(<1..219)
                     /locus_tag="PLO70_16435"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HQZ76108.1"
                     /translation="MKKIISVLMLSLALSSCDILSKLPTGTGITEAEAGQGIKEALGQ
                     GLVKAVLNLNTTDGFFGSQFYKVLLPPDA"
     gene            complement(241..1251)
                     /locus_tag="PLO70_16440"
     CDS             complement(241..1251)
                     /locus_tag="PLO70_16440"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQZ76109.1"
                     /translation="MTTGELQQLLFKAIKNKIGENQSVAEEIATLLGISSDSAYRRMR
                     GEKTISLEELQILCTHYKVSIDHLMGLQSGGIVFQGQYLDKNNFRFEEYVTSMLHNLA
                     YMNSFKEKTFYYMCKDLPIFHQYHLRELAAFKWFFYLKTYFQFPEFERKKFRYADHPD
                     ELYALEKKVLDLYNQIPSVEVWNMESMNMFFRQIEFYRDGQVFESDNDVYLLYDAIGK
                     MWDHLEKQASLGYKFNYGDTDQKRIGEFKMYFNEVLMGDNNMLVELDGTKLSYVSHST
                     INFMLTRDPAFNENMYNHIQNQLRRSTLISEVSEKERARFFRIIRDRINTRKESLKTK
                     AV"
     gene            complement(1254..2249)
                     /locus_tag="PLO70_16445"
     CDS             complement(1254..2249)
                     /locus_tag="PLO70_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76110.1"
                     /translation="MPTTSSQDILFQRIKEILPQHISLVDAISEVLHVSSDSAYRRIR
                     GETPLVLEEAKTLCHQYNLSLDQLLEAKKGVILFRDYRLNNHQYDYRQYLQGLNEQLE
                     AVNNFMQKEIIYMSKDLPIFHNFYYRPLIAFRYYFWMKTHLMHPDFEKKQFHFDLLPT
                     DIEQMSFDLTKTYSRIPSTEMWNTESINSTISQIEFSKDSGHFQSSADIKIIYEALEE
                     TIYHMQQQAEYGCKFTPGEDPQGRKSNFKFFFNRVVLGDNTIMVITDFVKAAFINYGH
                     LNYITTSDESFCKELYSDFENLIRRSTQISQSSEKQRNIFFNIILSKIGDRKRNL"
     gene            2446..3159
                     /locus_tag="PLO70_16450"
     CDS             2446..3159
                     /locus_tag="PLO70_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76111.1"
                     /translation="MKRKLRFTSFLFFTSLLCHGQKKNYISLESGAYFGNANSNLETG
                     MVSSGFGDKESVSFLGLFTIDTEYPRKKAGKTSYRIKYGHYLNNKTAIEAGFGLSYYG
                     TVDGYDYKDFAQSNYLTLTSTINTLYIAWVKTDSPGKAGYGIGPALSLYKLSADMNRG
                     TTNQTKNYLLPGVMLKGFWHFIARPSWFVGMGTELTGTVPAKIDAITITTPSSVSKYK
                     SAKAGSFNGNTFITAGFKF"
     gene            3175..3675
                     /locus_tag="PLO70_16455"
     CDS             3175..3675
                     /locus_tag="PLO70_16455"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HQZ76112.1"
                     /translation="MKNIMKLSSIVIACFVLSLSACKKETDTSTSYLRGKVDGVAFEC
                     NSDIRATTGAAGDATIFFRGDVSPWSFRFYLDGQGSNISAGTYNFQTGIKHNAVLYQD
                     IHGYAAGYFCDIFAPCVFYGSGKITIQEINKKYIKGTFEYVTDLSAATGSFKTVTNGE
                     FNIKRG"
BASE COUNT         1163 a          752 c          696 g         1208 t
ORIGIN      
        1 tgcatcaggt ggtaataata ctttgtaaaa ctggctgccg aaaaacccat ctgttgtatt
       61 caggttcaac acggctttca ccaatccctg cccgagagct tctttaatac cctggcctgc
      121 ttctgcttca gtaatgcctg taccggttgg gagtttagat aaaatatcac aactgcttaa
      181 agccagtgat aacatgagta cagaaatgat ctttttcata atgtaagatt agaataataa
      241 ttatactgct tttgttttga gactttcctt tctggtgttg atgcggtccc ggataatacg
      301 aaaaaatcga gccctttctt tttcactcac ttcactaatg agcgtagatc tccgcaactg
      361 gttctgaata tggttataca tattttcatt aaatgccggg tctctggtga gcataaaatt
      421 aatggtggaa tgtgagacgt atgacaattt tgtaccgtcc agttctacca gcatattatt
      481 gtcacccatc agtacttcgt taaaatacat tttaaattca cctatacgtt tttgatccgt
      541 gtcaccataa ttgaatttat aacctaaaga tgcttgcttt tccaggtgat cccacatttt
      601 cccgatagca tcatacaaca aatacacatc attatctgat tcaaagacct gtccatcccg
      661 gtaaaattca atctggcgaa aaaacatatt catactttcc atattccaga cttccacact
      721 gggtatttgg ttgtaaagat caagtacttt cttttcaagt gcatataatt catcgggatg
      781 gtcagcatac ctgaactttt tcctttcaaa ctcgggaaac tgaaaataag tttttaaata
      841 gaagaaccat ttaaatgctg ccagttcccg cagatggtat tggtgaaaaa tgggaaggtc
      901 tttacacata taataaaatg tcttctcctt aaaactgttc atgtaagcca ggttatgcaa
      961 catactggtc acatattcct caaaccggaa attattctta tcaaggtatt gcccctgaaa
     1021 aacgatacca ccggattgta atcccatcag gtggtcaatt gataccttat aatgagtaca
     1081 aagaatttgc agttcttcca aagaaatagt cttttctccc cgcatacgcc ggtaagcact
     1141 gtcggaactg atacccagca atgttgcgat ctcctctgct acagactggt tttccccgat
     1201 tttattttta atagccttaa aaagcagttg ttgaagttcg ccggtcgtca taattataag
     1261 tttcgtttac gatccccgat tttagacaat ataatattga aaaaaatatt ccgttgtttc
     1321 tcgctgctct ggcttatctg tgttgaccgt cgtatcaggt tctcaaaatc actgtacaac
     1381 tctttacaaa agctttcatc actggtagtg atataattta aatgaccata atttataaaa
     1441 gcagctttta caaagtcagt tataaccatt attgtattat ctcccaatac aaccctgtta
     1501 aaaaagaact taaagttgct tttccggccc tgaggatctt caccgggtgt gaatttacag
     1561 ccatactcag cctgttgttg catatgatat atcgtttctt ccaatgcttc ataaatgatt
     1621 ttaatgtcgg ctgatgactg aaagtgcccg gaatcttttg aaaactcgat ctgtgaaata
     1681 gtgctgttaa tactttctgt attccacatc tctgttgatg gtatccttga atatgttttt
     1741 gtaaggtcga aactcatctg ttcaatatcc gttggcaaca gatcaaaatg aaattgtttt
     1801 ttttcaaagt cgggatgcat caggtgtgtt ttcatccaga aataataccg gaaagcgatt
     1861 agcggacggt aataaaaatt atgaaagatg ggaaggtcct tcgacatata gatgatctcc
     1921 ttctgcatga aattattgac cgcttccaac tgttcattta atccttgcag gtattgccgg
     1981 taatcgtact gatgattatt caggcggtaa tccctgaaaa gaattacccc ctttttggct
     2041 tccagtaact gatccagcga aagattatac tgatgacata gtgtttttgc ttcttccagc
     2101 accagcggtg tttctcccct gatccgcctg taggcactgt cgctgcttac atgcaaaact
     2161 tcagatatgg catctaccag ggaaatatgt tgtggtaata tctccttaat gcgctgaaat
     2221 aaaatatcct gtgaactggt ggttggcaaa tggttcaggt ttgatttttt gaaaaaactg
     2281 caacttgtct ccacaggttt gtaaaagata cttgatttta tgcaaatcat caatactggc
     2341 tgcatggtat ttagtaaaaa ctgcaaataa gggaaatgtt aaggcaaaat tcagtttccg
     2401 gtccgtataa tatgaattta gattgctgtt acaaaaacaa acaatatgaa aagaaagctc
     2461 cggttcacct cattcctatt ctttacatca ttactatgcc atggacagaa aaaaaattat
     2521 atcagtcttg aaagtggtgc ctattttggc aatgccaatt caaaccttga aacaggtatg
     2581 gtaagctcgg gttttggaga taaagaatcg gtaagtttct taggcctttt taccattgat
     2641 acagagtacc cgagaaaaaa agccggcaaa acttcttacc ggatcaaata tgggcactat
     2701 ctgaataata agaccgccat tgaagcaggt tttggtcttt catactacgg aactgtggat
     2761 ggatatgact acaaggattt tgcacaatcc aattatctta ctcttacaag tactatcaac
     2821 accttataca tcgcctgggt aaaaacagat agccccggaa aagcaggata cggtatcggc
     2881 ccggcattat ccttatacaa actctctgca gatatgaacc ggggtactac caatcaaacc
     2941 aaaaattatt tattgcccgg tgtcatgtta aaagggtttt ggcattttat tgcccgccct
     3001 tcgtggtttg tgggtatggg aacagaatta actggtaccg tacctgctaa aattgatgca
     3061 ataacaatta caacacccag ctcagtatcc aaatataaat cagcaaaggc aggttctttt
     3121 aacggcaata cttttattac agccggattc aaattctaag taaaaaatat aattatgaaa
     3181 aatataatga aactatcatc aatcgtcatc gcttgttttg ttttgtcctt aagtgcctgt
     3241 aaaaaagaaa cggataccag taccagttat ctgagaggta aagtagatgg cgtcgccttc
     3301 gaatgtaatt cagatatccg ggctactacc ggggcagctg gcgatgcaac tatttttttt
     3361 cggggcgatg tttccccctg gtcatttcga ttctatttag acggacaggg ttctaatatc
     3421 tcggctggca cttataattt tcaaacgggt ataaaacaca atgcagttct ctatcaggat
     3481 attcatggct atgctgccgg gtatttctgt gacatctttg cgccttgtgt attttacgga
     3541 agcggtaaaa taaccataca ggaaatcaat aaaaagtata ttaaaggcac ttttgaatat
     3601 gttactgact taagtgctgc aaccggcagt ttcaaaactg ttaccaatgg agaatttaat
     3661 attaaaagag gatagaaact gattgacaaa aaaggtcttt caataaaatt gactgtttaa
     3721 ctttaaaacc actaaatctt cctgttatga gaaatatatc agtatgggca aaaactcatc
     3781 aatggcccgc aagaattatt attattctta gcttcctgt
//
LOCUS       DAORTI010000228         3814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_31440_length_3814_cov_4.520351, whole genome shotgun sequence.
ACCESSION   DAORTI010000228 DAORTI010000000
VERSION     DAORTI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31440_length_3814_cov_4.520351"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLO70_16460"
     CDS             complement(<1..1467)
                     /locus_tag="PLO70_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQZ76113.1"
                     /translation="MKKTLLLLFTIVSFGAYAQINLNQGLIAHYPFNGNANDVSGNNI
                     NGTVSNATLTTDKAGTANSAYYFNGTNAHILLPFSNLYNFAPQDSFSISVWVAPEAGQ
                     SWIAQALVVKSPFHPDFTLSNWNYGVYLWDYTPMGGYALTHLLSSSIDTRIANPCWYN
                     IIMTYKNGIWKMYVNGILVSQDLTQTKFILQDGSSRIAFGRKGDAIGDWYKGKMDEIR
                     IYNRVLNKQEVDSIVGTCFIQCSQKNDFSFSQNPCSPNDVTFSTTVTGQSTLKWDFGD
                     GNIANTATTVTNTYTNPGNYQVTLITSYPHCTDTVRKTITIGIQNDNQLISTADTTIC
                     IGTTKQIITTPGLNFCWTPSTYLNNPNVANPISAPSQSITYYLQSLITGSNLIPNPDF
                     SAGNTGFTSQYTYTSNNTTDAEYFVGPSPQAWYFAHHPCTDHTTGNGNMLLVNGSATP
                     NLEVWRTNVSVTANTNYSFSVWICSISNPNPAQLAFSIN"
     gene            complement(1494..2837)
                     /gene="purB"
                     /locus_tag="PLO70_16465"
     CDS             complement(1494..2837)
                     /gene="purB"
                     /locus_tag="PLO70_16465"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQZ76114.1"
                     /translation="MELTALTSISAIDGRYRKQVQHLDEYFSEYGLMKYRILIEIEYL
                     LFLEKKKFFKLPGKALKHLSKLAEDFGPEDAQEIKQIEFTTNHDVKAVEYFLKNELEK
                     SGAATAKEWVHFGLTSQDINNTAIPLSWKHAIEYEYLPALLNLNTELKILANNWKDIP
                     MLAHTHGQPASPTKLGKEIMVFVERIENQIEQFIGIPFSGKFGGATGNFNAHHVAFPK
                     TDWIKFGNEFLQSLGLNRQQYTTQIEHYDSLAAHFDCMKRINNILVDLCRDTWTYVSM
                     EYFTQKTKKGEVGSSAMPHKVNPIDFENAEGNLGLANAIFEHLAAKLPVSRLQRDLTD
                     STVLRNIGVPFAHTLIAFRSIEKGLNKLVINDAKIYDDLDNNWAVVAEAIQTVLRREN
                     YPNPYEALKELTRGNNKIDQKAIHKFIEGLKVSEILKKELKKITPHNYTGVSSFK"
     gene            2895..3638
                     /locus_tag="PLO70_16470"
     CDS             2895..3638
                     /locus_tag="PLO70_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HQZ76115.1"
                     /translation="MDKIRIGIVNYLNTKPLIYGLEKPPISNQIELVGDYPARVAEML
                     LKGEIDVGLIPVAVIPQLPSYHIVGNYCIGTEGEIASVALFSEVPMQEIEQVYLDYQS
                     RTSVALLKFLMKEYWGINPKIIHAENEEYRQEIKGTTAGLVIGDRALEQRKISTFIYD
                     LGSEWKKITGLPFVFAAWVSIKELPAEFVRLFDEANAMGLEHIDEIIAENSYTLFDLK
                     KYYTLHLSYHLDEKKKKGMKRFLEVINEK"
BASE COUNT         1081 a          691 c          822 g         1220 t
ORIGIN      
        1 attaattgaa aaagcaagtt gtgcagggtt aggatttgat atagaacaaa tccaaacaga
       61 gaaagaatag ttcgtattag ctgttacaga aacgtttgtt ctccaaactt caagattagg
      121 agttgcagaa ccattgacca aaagcatatt tccatttccc gttgtatgat ctgtgcaggg
      181 atgatgggcg aaataccatg cctggggact gggtccaaca aaatattcag catcggtagt
      241 attattgctt gtataagtgt attgagaagt aaatcctgtg ttgcctgcac taaaatcagg
      301 gttcgggatg aggtttgatc ctgttatcag cgattgtaaa taataggtta ttgattggct
      361 gggagcactg ataggatttg caacattagg gttgttcagg taagtagaag gtgtccagca
      421 aaaatttaat cccggggttg taattatctg ttttgttgtt ccaatacaaa ttgttgtatc
      481 tgctgttgaa ataagttggt tatcattttg tatgcctatt gtaatagttt tcctgacagt
      541 atcagtgcaa tgaggataac ttgtaattaa tgtgacctgg taattacccg gatttgtata
      601 ggtgtttgtt acagttgtgg ctgtattggc aatattgcca tctccgaaat cccattttaa
      661 agtactttgc cctgtaactg ttgttgaaaa agtaacatca tttggagaac atggattttg
      721 gctaaaggaa aaatcatttt tctgagagca ttgaataaag catgtaccaa caatggagtc
      781 tacttcctgc ttatttagta ccctgttata aattctgatc tcatccattt ttcccttata
      841 ccagtctcct atggcgtcac cttttctgcc aaatgcgatc ctcgaagagc catcctggag
      901 gataaatttt gtctgtgtaa ggtcttgcga aacaagtatc ccgttaacat acattttcca
      961 tatcccattt ttgtaggtca tgatgatatt gtaccaacat ggattagcta tgcgtgtatc
     1021 aatactgctg ctaagtaaat gtgtaagggc atacccaccc atcggggtgt aatcccataa
     1081 ataaacccca tagttccaat tcgaaagtgt aaaatcagga tgaaaaggag acttgactac
     1141 aagggcctgt gcaatccagg attggccagc ttcaggtgct acccatacag aaatagaaaa
     1201 agaatcttga ggagcaaaat tatacaagtt agaaaaaggc aagagaatgt gagcattggt
     1261 accattaaaa taatatgcac tattcgctgt tcctgctttg tctgtggtta aagttgcatt
     1321 actgacagtt ccattaatat tatttccgct tacatcattg gcatttccat taaaaggata
     1381 atgtgctatc aatccctggt tgagatttat ctgggcataa gccccaaaac tgacaattgt
     1441 aaatagcagt aataaggttt ttttcatgaa cggtcagata gtcgggagtg aatttactta
     1501 aaagaactga cccctgtata attatgcggg gttattttct tcaattcctt ttttaatatt
     1561 tcgctgacct tcagtccttc gataaattta tgaatcgcct tctggtcaat tttattatta
     1621 cccctggtga gctcctttaa tgcctcgtaa ggattaggat aattttcacg gcgtaaaaca
     1681 gtttgaattg cttcagctac cactgcccag ttattatcga ggtcgtcata aattttagca
     1741 tcattgatca ccagtttatt cagccctttt tcaatggagc ggaaggcaat caatgtatgg
     1801 gcaaacggca caccaatatt tcttaataca gtggaatcag ttaggtctct ttgtaatctt
     1861 gatacaggca atttggcagc aagatgttca aagatcgcat tggccaggcc gaggttgcct
     1921 tctgcatttt caaaatcaat cggattcact ttatgcggca tggccgatga acccacttct
     1981 cctttcttgg ttttttgggt gaagtattcc atggaaacat aggtccacgt atcgcggcag
     2041 agatctacca gtatattatt gatccgcttc atgcaatcga agtgagcagc aaggctgtcg
     2101 taatgttcta tctgcgtggt gtattgctgc ctgttcagtc ccaggctttg caggaattca
     2161 ttgccaaatt ttatccagtc tgttttgggg aaagccacat ggtgtgcatt gaagttgcct
     2221 gtagcgccgc caaattttcc gctgaaagga atgccgataa attgttctat ctggttttcg
     2281 atcctctcca caaataccat tatttccttg cccagttttg tagggctggc cggttgtccg
     2341 tgtgtatgtg ccagcatggg aatatctttc cagttgttgg ccagtatttt taattccgta
     2401 ttgaggttaa gtaaagcggg caggtattca tattcaatgg catgtttcca cgataaggga
     2461 atagcagtat tattaatatc ctgcgaggta agtccgaaat gtacccattc ctttgcagtg
     2521 gcggcaccgg atttctccaa ctcgttttta aggaaatatt cgaccgcttt tacatcatgg
     2581 ttggtggtga attcaatttg tttgatctcc tgtgcatctt ccggtccaaa atcctctgcc
     2641 agtttactaa ggtgctttaa ggcttttccg ggaagtttga aaaatttctt cttttcgaga
     2701 aaaaggaggt attcaatttc tatcaatatc ctgtatttca tcagtccata ttcagaaaaa
     2761 tattcatcca ggtgctgtac ttgtttgcga tagcggccgt caattgctga aatggaggtg
     2821 agagctgtaa gttccatgat tatgttttat agacgaattg agttccttta cctttgcccg
     2881 caaaaataac ccaattggat aagattcgta tcggcattgt caactacctt aataccaaac
     2941 cattgatcta tggcctggaa aaaccgccca tttccaacca gatagagctg gtgggtgatt
     3001 acccggcccg ggtggctgaa atgctgttga aaggtgaaat tgatgtgggg ttgataccgg
     3061 tggcggttat tccccagtta ccctcgtacc atattgttgg aaattattgt attggtacgg
     3121 aaggggagat cgcttccgtg gctttattca gtgaagtgcc gatgcaggag attgagcagg
     3181 tgtacctcga ttatcaaagc agaacatcgg tagcgctgct gaaattcctg atgaaggaat
     3241 attggggcat taacccgaaa attattcatg ccgaaaatga agaataccgg caggaaataa
     3301 aaggaacaac tgccggttta gtgatcggtg acagggccct tgaacagaga aagatctcta
     3361 catttattta tgacctgggt tctgaatgga aaaaaattac aggattgccc tttgtatttg
     3421 cggcctgggt aagtattaaa gaattgccgg ctgagtttgt ccggctattt gatgaggcta
     3481 atgcgatggg cctggagcat attgatgaga taattgctga aaattcttat actctttttg
     3541 accttaagaa gtattataca ttgcacctga gttatcacct ggatgagaag aaaaaaaaag
     3601 ggatgaaaag atttttggaa gtaattaatg agaaataagt ttaaataaat ttcatatcag
     3661 gttatacgaa ttttttatgg gcatctttac gacagaaaat agtattgaca tatgtgtcgg
     3721 cataaacaaa ataaccattg ctttcgaaaa aataaattat ttccttgttt tttgtttcca
     3781 tattgtgttc tataaattca aaggtctcta taca
//
LOCUS       DAORTI010000229         3801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_31601_length_3801_cov_4.506941, whole genome shotgun sequence.
ACCESSION   DAORTI010000229 DAORTI010000000
VERSION     DAORTI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31601_length_3801_cov_4.506941"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLO70_16475"
     CDS             complement(<1..869)
                     /locus_tag="PLO70_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQZ76116.1"
                     /translation="MAEYHIKHITRYTYPAPVIDSANQVMLFPINDDQQEVKKYELII
                     SNHPGVEVFTDYFGNKVGIFSIIKPHTELLIQSNIEVITREVQLPANDTAPETQWNNL
                     AVIREEFPYMDFMLQESFDAHDEVVAVVQSMMNETITPFTTAQLMSAFVFDTFEYKKG
                     ITSVETQVDEIWQLKAGVCQDFAHILLVMLRYVGIPARYVSGYICPKNHELRGEGATH
                     AWVEAYIPFHGWMGFDPTNNCIVSDRHVKLAIGRSFTDCTPVKGTYKGSSEHKLEVSV
                     VINNSSLKEEETI"
     gene            complement(890..1825)
                     /locus_tag="PLO70_16480"
     CDS             complement(890..1825)
                     /locus_tag="PLO70_16480"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQZ76117.1"
                     /translation="MLSRVADSVFWMARYMERTNGMLRILRTNYISSQDEVKDFSWQT
                     LLQNYSDAPPEDVKAIGDHSVKVLEHLLFDKNNGGSVVNYITRARENARAVQDHITKE
                     VWQCLNDYYHLIRDKQIHFQVQYGDPVTALDTLIHHGMLYHGTVDITMARGEGFNYLN
                     IGKFLERAVLSADMLNIKLTELNYNLQQPVEVPALRYLLYSLSGYELYLKTHRGNFQA
                     DNVLQQIIYNDNFPHSVSYCLKQLFRYFERLETESLPESYKRLEFMIGRSMNNVKYST
                     INTADGEGLKRFLQQTRQELFGIAAAFNQYYFGNS"
     gene            complement(1927..3372)
                     /locus_tag="PLO70_16485"
     CDS             complement(1927..3372)
                     /locus_tag="PLO70_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQZ76118.1"
                     /translation="MEGHFLLADYKVQPRVWDEMFYEGGVRSTYQNFVTAIQGLSADE
                     MTHKDELAKKLFMSQGITFTVYSSGEGIEKIFPFDIIPRILEAAEWQHIEEGIKQRLK
                     ALNIFLKDIYNQQFIIKDGIIPAKLIYSCPNFVRQMVNVDVPFDIYTHISGIDLIRDY
                     DGTYYVLEDNLRTPSGVSYMLENRSITYRIFPDLLPKNNVRSVKDYPDLLLKNLVALA
                     NRQKSDPTVVLLTPGIYNSAYFEHTTLARLMGIELVEGSDLVVDDHKVYVKTTTGLKQ
                     VDVIYRRVDDDFIDPLVFQPDSMLGVPGIYGAYRTGHVAIVNAMGNGVADDKAVYSYV
                     PAMIRYYLNEEPILKNVPTYQLAIPENRKQVFDSMDKMVIKKTNESGGYGMLIGSAAT
                     EKEMEDFKVAIEEDPRSFIAQPIISLSSAPCYINGLLQPRRVDLRPYALYGPEGIDIV
                     PGGLTRVALKEGSLVVNSSQGGGSKDTWVLG"
BASE COUNT         1116 a          783 c          703 g         1199 t
ORIGIN      
        1 tgtatagtct cttcttcttt taaggagctg ttatttatta cgacagatac ttccagtttg
       61 tgttcggagg atcctttata agttcctttt accggggtac agtcagtaaa acttctgccg
      121 atagccagtt ttacatgtct gtcacttaca atacaattat tagttgggtc aaaccccatc
      181 cagccatgaa aaggaatgta ggcttctacc caagcatggg tagcaccttc gccacgaagt
      241 tcatggttct taggacatat atacccgctt acatagcggg cgggtatacc tacataacga
      301 agcattacca gtaatatatg tgcaaaatcc tggcaaacgc cggctttcag ctgccatatc
      361 tcatctactt gtgtttccac acttgtaatt ccctttttat attcaaatgt atcgaaaacg
      421 aaagcagaca tcagttgtgc agtagtaaat ggtgttattg tttcattcat cattgattgt
      481 acaactgcca ccacttcatc atgggcatca aaactttcct ggagcataaa atccatataa
      541 gggaattctt cccggataac agcgagatta ttccattgtg tttctggcgc tgtatcattg
      601 gccgggagtt gaacttctct tgtaataacc tcaatatttg actgaatcaa taactctgta
      661 tgcggcttga tgatcgagaa aatacccact ttattaccaa aataatctgt gaagacttca
      721 acacccggat gatttgagat gatcagctca tactttttta cttcctgttg atcatcattg
      781 atggggaaaa gcattacctg gtttgcacta tcaattacag gtgcaggata agtgtatcgg
      841 gtgatatgtt taatatgata ctcggccatg ggtacaaagt tgcggcaatt catgaattgc
      901 caaaataata ttgattaaag gcagctgcta ttccaaatag ctcttgccgg gtttgttgta
      961 aaaatctttt taatccttcg ccatctgcag tattgatggt actgtacttt acattattca
     1021 tagaccggcc gatcataaac tcaagtcgct tataactttc aggcaggctc tctgtttcca
     1081 gtcgttcaaa atagcgaaac agttgtttta aacaataaga aaccgaatga gggaaattat
     1141 cgttatagat tatctgttgc agtacattat ctgcctggaa gtttccacga tgagttttta
     1201 aatataattc gtaaccagat aaggaataca gcaaataccg gagagccggc acttcaacag
     1261 gttgctgcag gttataattt aattcagtaa gttttatatt cagcatatca gctgaaagaa
     1321 cagctctttc taaaaatttt ccaatgttca gataattaaa accttcaccc ctggccatcg
     1381 ttatatccac cgtgccgtgg tagagcattc cgtgatgaat taatgtatct aatgcagtta
     1441 caggatcacc atactgaacc tggaagtgaa tttgtttatc tcttatcagg tgataatagt
     1501 cattcaggca ttgccatact tctttagtta tatgatcctg tacagctctt gcattttctc
     1561 tggcgcgggt aatataatta acaactgaac ctccgttatt tttatcgaac aatagatgct
     1621 ccagcacttt aacggaatga tcaccgattg cttttacatc ttccggaggc gcatcgctgt
     1681 aattctgcag caaagtttgc caactaaagt cttttacctc atcctgggaa gaaatatagt
     1741 tggttcgaag tatccgcagc attccattgg tccgttccat ataacgggcc atccagaaaa
     1801 cagaatcagc aactctactt aacatgatct cttctttatt atttttttct caactatgtt
     1861 ctattagcta caggatttta aaatctagcc tagattgatc tttcaaatct gaatcgaaac
     1921 acggaactat cccagtaccc aggtgtcttt acttccgcca ccctgtgatg aatttacaac
     1981 caatgaacct tcttttaatg ccactcttgt caaccctccg ggaacaatat caattccctc
     2041 cggtccatat aacgcataag gccttaaatc tactcttctg ggttgtagta atccgtttat
     2101 ataacatggc gctgatgata aactgataat aggctgggca atgaaacttc tcgggtcttc
     2161 ttcaattgct actttaaaat cttccatttc tttttcggta gctgcactac cgattagcat
     2221 tccatacccg ccagattcat tcgttttttt aattaccatc ttgtccatac tgtcaaaaac
     2281 ctgtttcctg ttttcaggaa tagctaactg ataagtggga acatttttta aaataggttc
     2341 ttcgttcagg taatatctga tcatagccgg tacatatgaa taaacagctt tatcatctgc
     2401 cacaccatta cccatggcat tcacaatagc aacatgacct gttctgtatg cgccatatat
     2461 tcccggtaca cccagcatac tatccggctg gaaaactaac ggatcaataa agtcatcatc
     2521 caccctccgg taaatgacat ctacctgttt taaaccagtt gtggttttca cgtacacttt
     2581 atgatcatct acaacaaggt cgctgccttc caccagttca attcccatca atcttgccag
     2641 agttgtatgt tcaaaataag cagaattata aatgccgggg gtaagcaata caaccgtcgg
     2701 atcacttttc tggcgattgg ctaatgcaac cagatttttt aacaagaggt ccgggtaatc
     2761 ttttactgac cgtacattat tttttggtaa gaggtcagga aagatgcggt aagtaatact
     2821 cctgttttcc agcatatagg agacaccgga aggtgttcgt agattatctt ctagaacata
     2881 ataggttcca tcatagtcgc ggataaggtc aattccggag atatgagtgt agatatcaaa
     2941 tggcacatcc acattcacca tctggcggac aaaattcggg caggaataaa taagtttggc
     3001 cggaatgatc ccatctttaa taatgaactg ctggttataa atgtctttaa ggaaaatatt
     3061 cagtgctttc agccgttgtt tgataccttc ctcaatatgt tgccattctg cggcttccag
     3121 gatccggggg atgatatcaa aagggaatat tttctctatg ccttcgccgc tgctgtacac
     3181 agtaaatgtg atgccctggc tcataaataa tttctttgcc agttcatctt tgtgggtcat
     3241 ttcatcagca gataaaccct gtatggcagt tacaaaattt tgataggtgg agcggacgcc
     3301 accttcataa aacatttcat cccatacccg gggttgtact ttataatcag ctaaaagaaa
     3361 gtgtccttcc atacgtaaat gaatttacga agaataaata aaagctatat ctctgtggaa
     3421 gtaatcgtta ggaagctttg ttgggtctgt tgcttcgtta aaggaaagat aagatcgaaa
     3481 actggattaa acaatttatc aaaagtataa ttatacgaac tgtcgaaact tgtttgccct
     3541 gaactgtgtt gtatttgaaa gtaagggagg atagaaaatg aaaaacattc tgatcataaa
     3601 agagcaggga agccaatgat tttttagcac ttttcggaac ttcgatcatg tctatattgc
     3661 tatcacaaaa ccggagaggt tgaataaaaa gaacaataat acggtactaa tcaaagattt
     3721 tgatgcgcca ggtcacggtt tagaaatttc tgttttcttt tacccaattg ataatataat
     3781 cagcgatctc atgtgtattg g
//
LOCUS       DAORTI010000230         3764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_32112_length_3764_cov_7.607981, whole genome shotgun sequence.
ACCESSION   DAORTI010000230 DAORTI010000000
VERSION     DAORTI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32112_length_3764_cov_7.607981"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLO70_16490"
     CDS             <1..494
                     /locus_tag="PLO70_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76119.1"
                     /translation="ELFPSYISRRFAPEFITANCMKAVAEELFPDQSGGKPLIEQMIE
                     KGKLWYLLDKFLPSTPDSIKTGYTGRQLNWCSENEGLIWSYLVKNEDLNSLSPAVIQA
                     YIGEAPFTQGFSQELSPGNIGQWIGWQIIKKYVSKNPEMKPEEIMRTEAKKILDEAKY
                     KPK"
     gene            594..1646
                     /locus_tag="PLO70_16495"
     CDS             594..1646
                     /locus_tag="PLO70_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQZ76120.1"
                     /translation="MKFLSPTLTDPVYVPKQQYSLYDKFWLRIMNDKRDLPFIYLLTK
                     IHILVVPVAILLFTPVLTGWWWWAVAIPYFYVSQLYFKGRFGLMFHCLCHRKTLKAPY
                     QQPFHTYITWILCPLFGHAPEGYFSHHMGMHHIENNLPDDTSSTMPYQRDSLKDFLAY
                     FFKFIFRGVIDTIRYLFVRKRKKLYQRLTIGEYVYMAFCIALCFVNLKATLVVFIIPL
                     LFARLVMMLGNWTQHSFVDPNEPDNLYKSSINCINTVYNKTCWNDGYHIIHHLRPGMH
                     YTEMPEEFLKRKDEFAENKAIVFDGIHYLHIFTWLLTKRYDKLADNLVNINGNMFQSA
                     EEAIALMKERTKKIGN"
     gene            complement(1643..2029)
                     /gene="apaG"
                     /locus_tag="PLO70_16500"
     CDS             complement(1643..2029)
                     /gene="apaG"
                     /locus_tag="PLO70_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQZ76121.1"
                     /translation="MTSMISEGVQVSVETFYQADYSNPLQSEFMFAYRITLENHNSFP
                     VKLHRRHWYIFDSNGSHREVEGEGVVGVQPVLQPGENYQYVSGCNLRTEMGKMYGTYQ
                     MENLHSKKMFDVSIPSFEMIVPMKNN"
     gene            2135..3586
                     /locus_tag="PLO70_16505"
     CDS             2135..3586
                     /locus_tag="PLO70_16505"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQZ76122.1"
                     /translation="MKIAVAKGDGIGPEIMEAVLKIFKANNVPLEYDFVDMGKWVFDK
                     GFNNGMTPEAKQTIEALGLLFKGPMETPKGKGVKSINVTARKTWNTFANKRDFRTLHG
                     VDTVFSKAGIPIDITVVRENIEDTYGGIEHMLTHDVALSRRFITRPGSMQVIRYAFEM
                     ARQKMAKRITCVHKANIMKITDGLFLECFQEVAKEYPELKADDIIVDDLCMKLVTRPD
                     NFDVLVLTNLQGDIVSDLCAGLVGGLGFAPSANIGDHISIFEAVHGTAPDIAGKNIAN
                     PTALLLSGLAMLRHVGLTENAAIIENALLYTLENGVHTGDFGDKTKPAVNTTQFADAI
                     ISNFGKKPEVGAREIIANKPGTPTPLKLETNPMMISKEGEAERIVGVDLFIESEEQPT
                     IIAEKCKRHGGVKFNLINISNRGTQVWPTGSVYTNLVNQYNVRFESINDEPLNQQDVI
                     GLYVSMSGNFKICSFELLNMWGDKKAYSLAQGQ"
BASE COUNT         1192 a          719 c          773 g         1080 t
ORIGIN      
        1 aggagctttt cccttcttat atttcaaggc gtttcgctcc tgagtttatt actgctaact
       61 gtatgaaagc agttgcagaa gaactttttc ccgaccaaag cggcggcaaa cccctgatag
      121 aacagatgat tgaaaaaggt aagttgtggt acttgctgga caaattcctg ccttcaactc
      181 ctgattccat aaaaacagga tatacaggaa gacaactgaa ttggtgcagt gaaaacgagg
      241 ggcttatctg gagctacctg gtaaagaatg aagacctgaa ctcactaagt cctgctgtta
      301 ttcaggccta tattggtgag gcccctttta cacaaggttt ctcccaggaa ctttcacccg
      361 gcaatattgg tcagtggatc ggctggcaga tcatcaaaaa atatgtatca aaaaacccgg
      421 agatgaaacc ggaagaaata atgcgaactg aggcaaaaaa aatattagat gaggctaagt
      481 ataaaccaaa ataaacagga tcctgaaact gtttcattct caactatctt ttagtgaaat
      541 atcctttacg gtagtggcgt tcaggggaat aaattgcata catttacggc aagatgaagt
      601 ttcttagtcc cacactcacc gatccggttt atgttccaaa acaacaatac agtctttatg
      661 ataaattctg gctgcggata atgaacgata aaagggatct gccattcata tacctgctga
      721 caaaaatcca tatactggta gtgccggttg ccatactttt atttacacct gtcttaacag
      781 gctggtggtg gtgggcagtt gccattcctt atttctatgt ttcccagcta tattttaaag
      841 gtcgtttcgg gttaatgttt cattgtcttt gtcacaggaa aacactgaaa gccccttacc
      901 agcaaccatt tcatacctat atcacctgga tactttgccc actttttggc cacgcaccag
      961 aagggtattt cagtcaccac atgggcatgc atcatatcga gaataatttg cccgacgaca
     1021 ccagcagcac catgccgtac caacgggata gtctgaaaga ctttctggcc tactttttta
     1081 aattcatttt ccggggtgta attgacacaa tacggtatct gtttgtaaga aaaagaaaga
     1141 aattatacca gcgtcttaca attggtgaat atgtttatat ggcgttctgc attgcccttt
     1201 gctttgtaaa tctgaaagct acattagtag tatttattat tcccttgctt tttgcaaggt
     1261 tagtaatgat gctcgggaac tggacacagc attcatttgt tgatccaaat gaaccagata
     1321 acttgtataa aagttcaata aactgtatca acactgttta caacaaaacc tgctggaatg
     1381 atggttacca tatcattcat cacctccggc ccggcatgca ttacactgaa atgccggaag
     1441 aatttttaaa aagaaaagat gaatttgctg aaaacaaagc catcgttttt gacggcattc
     1501 actatctcca catttttacc tggttgttga caaaacgtta tgataaactg gctgacaacc
     1561 tggtcaacat taatggcaat atgtttcagt cggcggaaga agctattgca ttgatgaaag
     1621 agagaacaaa gaaaatcggc aactaattat ttttcatcgg cacaatcatc tcaaaagagg
     1681 ggatacttac atcaaacatt ttcttgctgt gcagattttc catctggtaa gtgccataca
     1741 tcttacccat ttcggtacgg aggttgcatc cgctgacata ctgataattt tctccgggct
     1801 gtagtacagg ctgaacacct actactccct ccccttctac ttcacgatga ctgccattgc
     1861 tgtcaaaaat ataccaatga cgccggtgta attttacggg gaatgagtta tgattttcaa
     1921 gagtgatgcg ataggcaaac ataaattctg attgcagtgg gttactgtaa tcggcctggt
     1981 agaaagtttc tacactcacc tgaacacctt cagaaatcat actggtcata aggtaaaaat
     2041 aatcaaaaag aatcagaagt gaattgtaca cttttttaat gctttaatat ccttccaggg
     2101 cttaattttg cgcctgattc aaataaaata atatatgaag atagctgtag ccaaaggcga
     2161 tgggattggt cccgaaataa tggaagccgt acttaagata ttcaaagcca acaatgtacc
     2221 gcttgaatat gattttgtcg atatgggaaa atgggtgttt gacaaaggat tcaacaatgg
     2281 tatgacgcca gaagcaaagc agactatcga ggcactcgga cttcttttca aaggcccgat
     2341 ggaaactcca aaaggaaaag gagtaaaaag tatcaatgtt accgccagaa aaacctggaa
     2401 cacttttgct aataaaagag atttcaggac ccttcacggt gtggatactg tatttagtaa
     2461 agctggtatt cctattgata ttacagttgt aagggaaaat attgaagaca cttatggtgg
     2521 cattgagcat atgctcactc atgatgtggc actcagccgt cgcttcatca ctcgtccggg
     2581 aagtatgcag gtgatccgtt atgcatttga aatggcaaga caaaaaatgg caaagcgtat
     2641 tacctgtgtt cacaaagcca atatcatgaa aattacagat gggttgttcc ttgaatgttt
     2701 ccaggaagta gcaaaagaat atccggaact gaaagcagat gatataattg tagatgacct
     2761 atgtatgaaa ctggtaacaa gacctgacaa ttttgatgta cttgttttaa ccaatttaca
     2821 gggtgatata gtatctgatc tatgtgcagg tcttgttggt gggcttggct ttgccccatc
     2881 agcaaatatc ggggatcata tttccatctt tgaagcagta catgggacag cacctgacat
     2941 tgcaggaaaa aatattgcca accctaccgc tttattatta agcgggctgg caatgcttag
     3001 acatgtggga cttacggaaa atgcagctat tattgaaaat gcattattgt atacgttgga
     3061 aaacggagtt cataccggtg attttggtga taaaacaaaa ccagctgtaa atactactca
     3121 gtttgccgat gcgattatca gcaattttgg caaaaagcct gaagtaggtg ccagggaaat
     3181 cattgccaac aagccgggca cccccactcc attgaaatta gaaacaaacc cgatgatgat
     3241 ttccaaagaa ggagaagctg aaagaatagt tggggtggat ttatttatcg aaagtgaaga
     3301 gcaaccaacc attattgcag agaagtgtaa acgacatggc ggggttaaat tcaaccttat
     3361 aaacataagc aaccggggta cacaggtttg gccaaccggt tcagtttata ccaacttggt
     3421 caaccagtat aatgtccgtt tcgaaagcat taatgatgag cccctcaatc agcaggatgt
     3481 aatcggactt tatgtaagca tgagtgggaa ttttaagatt tgttcattcg aattattgaa
     3541 tatgtggggt gataaaaagg cttacagttt agcacagggg caataatttt tcatttacac
     3601 ctgctcacag acattactat ttttaagttt aactacaact taacgtatgg cacggtttta
     3661 gctcatttta aaaagtacta aaatgagttg attcgctaaa tttttatgtt ctataatagt
     3721 ttagcaggtt caattatatt ctaaaaaacg gttagtaagt aaaa
//
LOCUS       DAORTI010000231         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_32565_length_3730_cov_5.767891, whole genome shotgun sequence.
ACCESSION   DAORTI010000231 DAORTI010000000
VERSION     DAORTI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32565_length_3730_cov_5.767891"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLO70_16510"
     CDS             <1..544
                     /locus_tag="PLO70_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76123.1"
                     /translation="DLPVTTQIINGKAMRTDVEVMGQSITNVYYNGTGWKINPFAGAE
                     TATEVTGTELIDFKGQASLVSNLMDYKNRGHLVELLAPEEMDGITVQKLKLTSKEDGR
                     ITTYYINAATSLVHKSVTKREMQGQEFDFETYYSDYKEFGGIKFAMSRSQKVEGQVVQ
                     EMNMEKVELNAAIDESIFKM"
     gene            complement(601..1032)
                     /locus_tag="PLO70_16515"
     CDS             complement(601..1032)
                     /locus_tag="PLO70_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HQZ76124.1"
                     /translation="MINISYPEPAFRIKKENGKEFIFDPLRKKWLVLTPEEWVRQNFL
                     QYLLQVKKYPAALIAVEKEILLGELKKRFDILVYNKEYQPWMMVECKAMEIKLDEMVL
                     EQVLRYNISVPVEFMVITNGNLVYAWQKKDDELILINELPV"
     gene            1064..1834
                     /locus_tag="PLO70_16520"
     CDS             1064..1834
                     /locus_tag="PLO70_16520"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HQZ76125.1"
                     /translation="MKTKEEIVHNWLPRYTGEKLENFGEYILLTNFGNYVHMFAEWNN
                     VQVIGEDKPMQCATANNISIINFGMGSPTAATIMDLLTAINPKAVLFLGKCGGLKKRN
                     KLGDLILPIAAIRGEGTSNDYFPAEVPALPAFALQKAVSTTIREYEVDYWTGTVYTTN
                     RRVWEHDEPFKEYLQKVRAYAIDMETATIFIVGFYNKIPTGALLLVSDQPMIPEGVKT
                     EESDKKVTASFVENHLKIGIDSLKQLINDGLTVRHLKF"
     gene            1845..2219
                     /locus_tag="PLO70_16525"
     CDS             1845..2219
                     /locus_tag="PLO70_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76126.1"
                     /translation="MKLFFLLTTVFLISVSAENCTKESTKKTYKGRLEIAGICMNYTI
                     SVLEGNIDTSLIVADWTDDVTGKTYQNVFKLGSPCTFPATIKQGDEFYFVIDSSQAQN
                     CAVCLAYYPTPKKTLPIKIVTP"
     gene            2219..>3730
                     /locus_tag="PLO70_16530"
     CDS             2219..>3730
                     /locus_tag="PLO70_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76127.1"
                     /translation="MHPLLSIKNLSVDFIAESGVTHALKNISINVNRGEIVALVGESG
                     SGKSVTSLSILQLLPSPPAKYSTGEIIFSENGNDATDLLKRNRYEMQDIRGNKIAMIF
                     QEPMTSLNPVLTCGDQVMEAILLHKQVSKENAKLQTIAWFEKVKLPDPESIFKRYPHQ
                     LSGGQKQRVMIAMAMSCEPSLLICDEPTTALDVTVQKTILQLIKELQQQSNIGVIFIT
                     HDLGVVAEVADRAVVMYKGEIVEQHTVKEIFTNPKHPYTQALLACRPVNHERGKRLPV
                     VSDFLQPADSIAQPLASISKSKNNSSSLRSSPEALLTVENLSVWFPTKKTWLGKPVSF
                     TKAVDNVSFEVYKSETLGLVGESGCGKTTLGRALLRLIEPTTGKIMYNNLDLTSKKRD
                     ELQNLRKDIQIIFQDPYSSLNPRLTIGSAIAEPMKVHHILTNEKQRKEKVTELLEKVN
                     LKAEHFSRYPHEFSGGQRQRIVIARALALNPSFIICDESVSALDVSVQAQVLNL"
BASE COUNT         1226 a          782 c          748 g          974 t
ORIGIN      
        1 ggacctgccg gtaactaccc aaattattaa tgggaaagcc atgcgtactg atgtggaagt
       61 aatgggacaa tccatcacta atgtgtatta taatggcacc ggatggaaaa taaacccttt
      121 tgccggcgca gaaacagcaa ctgaagttac cggtactgaa ctgatcgatt ttaaaggaca
      181 agccagtctt gtcagcaacc tgatggacta taaaaacagg ggtcacctgg tagaattact
      241 ggcacccgaa gaaatggatg gcataaccgt tcaaaaactg aaactcacca gcaaggaaga
      301 tggccggata actacctatt acattaacgc tgcaacttcc ctggtgcata aatctgttac
      361 gaaacgggaa atgcagggac aggaatttga ttttgaaact tactattccg attataaaga
      421 atttggcggt attaagtttg cgatgtcaag aagccagaaa gtagaaggcc aggtagtaca
      481 ggaaatgaat atggaaaaag tagaactgaa tgctgcaatt gacgaatcta tttttaagat
      541 gtaagatata aaaatataaa ctatataaaa tccttccgta tcccggaggg atttttagtt
      601 ttatacaggg agctcattta tcaaaattag ttcgtcgtct ttcttctgcc aggcatacac
      661 taaattacca tttgtaatca ccatgaactc gacaggtaca cttatattat aacgaagtac
      721 ctgctccaat accatttcat ccagctttat ttccatggcc ttgcattcca ccatcatcca
      781 tggttgatac tccttattgt aaacaagaat atcaaatcgc tttttcaact cacccaaaag
      841 aatctccttt tcgacagcta ttaaagcagc gggatatttt tttacctgta acaggtattg
      901 aagaaaattc tgccgcaccc attcttccgg cgtcagtacc agccattttt tacgaagcgg
      961 atcaaatata aactccttac cattctcctt ttttatccgg aatgccggct caggataact
     1021 gatattgatc atgcttcaaa agtaattgat taaattcgcc tttatgaaga caaaagaaga
     1081 aatcgtacac aactggctac cccgttacac cggtgaaaag ctggaaaatt ttggcgagta
     1141 catacttttg accaacttcg gaaactatgt tcacatgttt gccgaatgga ataatgtgca
     1201 ggtcattgga gaggataaac ctatgcaatg tgcaacagcc aacaatatca gtatcatcaa
     1261 cttcggcatg ggaagtccca ccgctgcaac gataatggat ctgctaactg ccatcaaccc
     1321 aaaagctgta ttattccttg gaaaatgcgg tggccttaag aaaagaaata aacttggcga
     1381 cctgatatta cccattgcgg caatcagggg cgagggtact tcaaatgatt attttcctgc
     1441 agaagttccg gcactgcctg cttttgccct acaaaaagca gtatcaacca cgatccgtga
     1501 atacgaggtg gactactgga caggcactgt gtacactacc aaccgcaggg tatgggaaca
     1561 tgatgaaccc tttaaagaat acctgcaaaa agtaagggca tatgccattg atatggaaac
     1621 agctaccatt tttatagttg gtttctacaa taaaatacca accggggcgc tgttgcttgt
     1681 aagtgaccaa cccatgatcc ccgaaggtgt gaagactgaa gaaagcgata aaaaagttac
     1741 agcaagtttt gtagaaaatc accttaagat cgggatcgat tccctgaaac aactgatcaa
     1801 tgacggacta acagtaagac atttgaaatt ttaaaccggt aaatatgaaa ctctttttcc
     1861 ttctgactac agttttcctg atatctgttt cagctgagaa ctgcactaaa gaaagtacaa
     1921 aaaaaacgta taaaggacgt ttggagattg cagggatctg catgaactat acaatcagtg
     1981 tactcgaagg gaatattgat acttcactga ttgtcgcaga ttggactgat gatgtaaccg
     2041 ggaaaaccta tcaaaatgtt ttcaagctgg gctcaccatg tacttttcct gcaactatca
     2101 aacaagggga tgagttttat tttgttattg acagctcaca ggctcaaaat tgtgcagtat
     2161 gcctggctta ctatcccact ccaaaaaaaa cactgcctat aaaaattgtt acaccataat
     2221 gcatccgctg ttaagtataa aaaatctttc agtcgatttt atagcagaat caggtgttac
     2281 ccatgcatta aaaaatataa gcatcaatgt aaaccgtggt gaaatagtag cattggtcgg
     2341 cgaatcaggt tccggcaaat ctgttacttc tctttctata ttacaattac ttccatcccc
     2401 tcctgcaaaa tattccaccg gagaaattat attttctgaa aatggtaatg atgcaactga
     2461 ccttttaaaa agaaatcggt acgagatgca ggatatacgt ggcaataaga tagccatgat
     2521 attccaggaa ccaatgacct cattgaaccc ggtgcttacc tgcggtgacc aggtaatgga
     2581 agctatcctg ctgcacaaac aggtttcgaa agaaaatgca aaattacaaa ccatcgcctg
     2641 gtttgaaaaa gtgaaactgc ccgaccccga atctattttt aaacgctacc cgcatcaact
     2701 aagcggcggt caaaagcagc gggtaatgat tgcgatggct atgtcatgcg aaccttcctt
     2761 attgatctgc gatgaaccaa ctacggcact ggacgtaacg gtgcaaaaaa caattctgca
     2821 actgataaag gaactgcagc aacaatcaaa tataggtgtc atattcatta cccatgacct
     2881 tggagtggtg gctgaagtag cagacagagc cgtggtaatg tataaaggag aaatagtgga
     2941 acagcataca gtaaaagaga tttttaccaa tccaaaacat ccgtatacac aagccttgct
     3001 ggcctgccgg ccggtgaacc atgagcgggg taaaagactt cccgtagtca gtgatttttt
     3061 gcagcctgca gatagcatcg cacaaccctt agccagcata tcaaaatcga aaaataattc
     3121 gagttcccta aggtcatcac cggaggccct tcttactgta gaaaacttat ctgtctggtt
     3181 tcccacaaaa aaaacatggc tgggcaaacc agtttctttt accaaagcgg tcgacaacgt
     3241 aagttttgaa gtttataaaa gtgaaacgct tggattagtt ggtgaaagtg gctgtggcaa
     3301 aacaacattg ggcagggcat tattacgatt gatagagccc accaccggaa aaattatgta
     3361 taataatctc gatctgacct ctaaaaaaag agacgagtta caaaacttaa gaaaggacat
     3421 ccagataatc ttccaggacc cctactcctc gctgaatcca cgtttaacta ttggttctgc
     3481 aattgccgaa cccatgaaag tccatcatat tcttaccaat gaaaaacaac gaaaggaaaa
     3541 agtaaccgag ttactggaaa aagtaaacct gaaagcagaa catttctccc ggtatccaca
     3601 tgaattcagc ggtggtcagc ggcagcgaat agtaattgca agagcactgg cactcaatcc
     3661 atcttttatt atttgtgatg aatcagtttc tgcactggac gtaagtgtgc aggcccaggt
     3721 tctgaattta
//
LOCUS       DAORTI010000232         3640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_33802_length_3640_cov_7.089261, whole genome shotgun sequence.
ACCESSION   DAORTI010000232 DAORTI010000000
VERSION     DAORTI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33802_length_3640_cov_7.089261"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1304)
                     /locus_tag="PLO70_16535"
     CDS             complement(231..1304)
                     /locus_tag="PLO70_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HQZ76128.1"
                     /translation="MKFRNFKMVGYVVYGRGSFNQVDEIIAPHRKEGKPMVFLVDHFF
                     EGKPLVSRIPVRGNDSIIFADVTYEPKTTYVDKLAQQLKEEFGTVSGVIGIGGGSTMD
                     LAKAVSLMMNNPGSSADYQGWDLVKYPGVYKAGIPTLSGTGAEVSRTTVLTGPTKKLG
                     MNSDFTPFDQIVLDPELTKEAPANQRFYTGMDCYIHCIESLEGTYLNEFSKSYGEKAL
                     QLCRDVFVNKEHWDDDCDDKLMMASYAGGMSIAYSQVGVAHAVSYGLSYLLGTKHGIG
                     NCIVMNHLEEYYPAGVKEFKYMVEKNKIDIPTGICKGLSEEDFEKMINVSMGMKPLWE
                     NALGKDWEKIMTREKLRALYEKL"
     gene            complement(1350..1733)
                     /locus_tag="PLO70_16540"
     CDS             complement(1350..1733)
                     /locus_tag="PLO70_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQZ76129.1"
                     /translation="MHNLETINDITGKIIGASFRIHSELGPGLLESSYKVCLYYVLTR
                     SGLFVEKEKALPLIFEEVKLDIGYRIDLLVENKVIVETKSVEALTDVHTAQVLTYLKL
                     TQCKVGLLINFNVADLKKGIKRLIL"
     gene            complement(1774..2490)
                     /gene="kdsB"
                     /locus_tag="PLO70_16545"
     CDS             complement(1774..2490)
                     /gene="kdsB"
                     /locus_tag="PLO70_16545"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQZ76130.1"
                     /translation="MKKIAMIPARYAATRFPGKLMQVLGDKTVIRHTYDATVATGLFD
                     EVIVVTDSDIIFTEITAAGGSAVLSKYEHESGSDRIAEAAAEMEVDIIVNVQGDTPFV
                     KKEPLQKLLQQFDDETVLVASMMQELTKQEEIDDPNFVKVAVDKNGNALFFSRSVIPY
                     PRDKDYPTTYYEHIGVYAFRKQALMNFTQWPVSPLEAAEKIECLRYLENGIPLRMIVV
                     DYMGVEIDTPEDLERAAGLL"
     gene            complement(2500..3273)
                     /locus_tag="PLO70_16550"
     CDS             complement(2500..3273)
                     /locus_tag="PLO70_16550"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQZ76131.1"
                     /translation="MKKLCLLIPLITGLLFSFMSFSQSSSFYTAAKSGLSLRENPAID
                     AKVIDKIPYGTKITLLTTDEEKKTIVTEGMEGSWEKVKYNNKTGYVINSYLFPLPPPK
                     LATVKEMKNYLAQVTTAFGARLVTKSGATDNSEGFGWELRKQLYKNGAEHHEFIGYEY
                     NSNTYFLPDFSLEQGFLLLRLIPEFKELFGEKDEFPSSSKKYVKGETEYEVTVYKRVF
                     DADHSWIDKIRIEYAEGAVYFFEMYLLDNQLVIFYSAGL"
     gene            complement(3300..>3640)
                     /locus_tag="PLO70_16555"
     CDS             complement(3300..>3640)
                     /locus_tag="PLO70_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQZ76132.1"
                     /translation="SGAPMYSVMIYQTLVVLATAFTHANISLPKGVDKAISYLLISPN
                     MHKVHHHWKQPYTDSNYGAVFSFWDRLLRTFTFLDTKDLRYGLDRYFPEEKDEDIVTL
                     LVKPFQKQDE"
BASE COUNT          953 a          874 c          690 g         1123 t
ORIGIN      
        1 agatttttca aaataaatac cgatgcactc attgccccgg tgttgcagtg gttttagtac
       61 aacttctgtc atcagataag gtttgtctga tgaatttact aaaactaatt aaagtataat
      121 gtaactgctt tttcttccgt tttttgcagt aagccgctac caataaagca gctcaaatct
      181 gttttttaga atttttccat cagcatgagc ctgttcccca ttttgcgggg ttacaatttt
      241 tcgtacaagg ctctcagctt ctcccttgtc atgatctttt cccaatcttt tcccagcgca
      301 ttttcccaga gcggcttcat acccatcgaa acattgatca ttttttcaaa atcctcttca
      361 cttaaccctt tgcagatacc ggtgggtata tcgatcttat tcttctctac catgtatttg
      421 aattccttta caccggccgg gtaatattct tccaggtggt tcatcactat acaattgccg
      481 atgccatgtt tggtgccgag taaataactg agaccatagc tcacggcatg cgccacccct
      541 acctggctgt aggcaatgct catacctccg gcataagagg ccatcatcag tttatcatca
      601 caatcatcat cccaatgctc tttatttaca aatacatcgc ggcagagctg taatgctttt
      661 tcaccataac ttttactgaa ttcgttcagg taagtgcctt ccaggctttc tatacaatgg
      721 atatagcaat ccatgccggt gtagaagcgc tggttggccg gggcctcttt ggtcagctcc
      781 ggatccaaca ctatctggtc aaacggagtg aagtcggaat tcatcccgag tttttttgtc
      841 gggcctgtca gtaccgttgt tcttgaaacc tctgcccctg tgccgctgag tgtgggaatg
      901 ccggctttgt atacgccggg atatttcacc aggtcccagc cctggtaatc cgctgatgaa
      961 cctgggttat tcatcatcag cgaaacagct ttggccaggt ccatggtgga accaccgcca
     1021 ataccaatta cgccgctcac cgttccgaac tcctctttca gttgctgtgc cagcttatct
     1081 acataggttg tcttgggttc atacgttaca tcggcaaaga tgatgctgtc atttccccgt
     1141 accggaatac ggctgaccaa tggcttgccc tcaaaaaaat gatctacgag aaagaccatc
     1201 ggttttcctt cttttcggtg cggtgctatt atttcatcta cctggttgaa agaaccccgg
     1261 ccatagacca catagccgac cattttgaag tttctgaatt tcatattttt taccacagat
     1321 tacacagatt attttcacgg ataacacagc tatagaatca gcctttttat cccctttttt
     1381 aaatctgcca cattaaaatt aataagcaaa cctactttac attgggttaa tttcaaataa
     1441 gttaatacct gggccgtatg aacatctgtc agcgcttcaa cagattttgt ttccacaata
     1501 actttgtttt ccaccagcaa atcaatgcga tagccaatat caagttttac ttcctcgaaa
     1561 attaaaggaa gagctttttc tttttcaaca aacagaccag acctggttaa aacatagtaa
     1621 aggcatactt tataggaact ttccaataaa cccggcccca attcggaatg aatcctgaaa
     1681 gacgctccaa tgattttacc ggtgatatcg tttatggttt ccaggttgtg caatctgtgt
     1741 gtatttaatc tgtgctatct gtggtttgat tacttataat aaaccagcag ccctctccag
     1801 gtcttcgggt gtgtcaatct ctacgcccat gtaatccacc acgatcatac ggagggggat
     1861 accattttcc aggtaacgga gacattcaat tttttctgct gcttccagcg ggctgacagg
     1921 ccattgcgta aaattcatca gggcctgttt gcggaatgca tacacaccaa tatgttcata
     1981 ataagtagta ggatagtctt tatctctcgg gtaagggatc acactgcggc tgaagaacaa
     2041 ggcgttccca tttttatcca ccgccacttt cacaaaattg ggatcatcaa tttcttcctg
     2101 tttggtcagc tcctgcatca tcgatgctac cagcacagtt tcatcatcaa attgctgcaa
     2161 caatttttgc aacggttctt tctttacaaa cggcgtgtca ccctgcacat tcacgataat
     2221 gtcaacctcc atctctgcag cagcttctgc aatacggtca ctgccacttt cgtgttcgta
     2281 tttacttagt acagcgctgc caccagctgc cgttatctct gtaaaaatga tatcgctgtc
     2341 ggttactact atcacttcat caaataaacc ggtagctacc gttgcatcat acgtatgacg
     2401 gataaccgtt ttgtcaccca atacctgcat cagcttgccc ggaaaccggg tggctgcata
     2461 acgggccggt atcattgcta tcttcttcat gctgcaaagc tatagaccag cgctgtaaaa
     2521 aattactaat tgattgtcga gcaaatacat ttcaaagaaa taaacagctc cttctgcata
     2581 ttcaatgcgg atcttatcaa tccaggaatg gtccgcatca aaaacccgtt tataaaccgt
     2641 tacttcatat tcagtttctc cttttacata ttttttgctg gaagagggga attcatcttt
     2701 ttctccaaac agttctttga attccgggat caagcgtaac aacaggaagc cctgctctaa
     2761 cgaaaaatcc ggcaggaaat aagtattgga attgtattca tatcctatga attcatgatg
     2821 ctcggccccg ttcttataca gctgtttacg caattcccag ccaaaacctt ctgaattgtc
     2881 cgttgctccc gatttggtga caagcctggc gccaaaagct gttgttacct gtgccaggta
     2941 gtttttcatc tctttcactg tcgccagttt tgggggcggc aggggaaaca agtatgaatt
     3001 aataacataa ccggttttgt tattatactt cactttttcc cagctaccct ccatcccttc
     3061 ggtgacgatc gtcttttttt cttcatcagt cgttagcagt gtaatctttg ttccataggg
     3121 aatcttatca atcacttttg catcaatagc cgggttttca cggagactta acccgctttt
     3181 ggctgctgta taaaatgaac tgctttgtga aaaactcata aaagaaaaga gcaaccctgt
     3241 aatcaacggg atcagcaagc ataacttttt catataaata agtttgatga atcgcagctt
     3301 tattcgtcct gcttttgaaa aggctttacc agcagggtca caatatcttc gtctttttct
     3361 tccgggaaat agcggtccaa gccataacga agatcttttg tatccaggaa tgtaaaggtt
     3421 ctcagtagcc ggtcccaaaa agaaaacaca gcaccatagt tactgtccgt atacggttgt
     3481 ttccagtgat ggtgcacttt gtgcatattg ggagaaataa gcaaataact tattgcctta
     3541 tccactcctt tgggcaaact gatattggca tgcgtgaaag cagtggccag taccaccagt
     3601 gtctggtaga tcattactga atacatggga gcaccggaaa
//
LOCUS       DAORTI010000233         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_34377_length_3602_cov_5.872286, whole genome shotgun sequence.
ACCESSION   DAORTI010000233 DAORTI010000000
VERSION     DAORTI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34377_length_3602_cov_5.872286"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLO70_16560"
     CDS             <1..1307
                     /locus_tag="PLO70_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76133.1"
                     /translation="DKKRRFIFFLLLFLFLGGAGIIIGKPFRPGKQVARAEEATGESK
                     SSKPAHNSFEGMGAGTTDEKTPTGIGKSEDIYPKAVKESTAVTDVDIDANSDYNNKNN
                     ILIAAKKYPAGQTRLNGSVNQQKKRQSYMQYDKPIDNAAVTSVDMQLDKPANNIVKPA
                     VVAITKNSITIDAADNNKEDVEIKEMSVKAGAEKIEVSDSVANKSAITIKKTQEKKKA
                     NGKAQKRNSFFLSFSAGPDVSYASSGKPGKIRPVAGLGLGYTFKDRLTVRTGFYSASK
                     VYSASPEAYHPPAVFYSYYPYLEKVDADCKVYELPLTFSYSFNHTKQQHWFASAGISS
                     YLMKTETYNYFYKTSPLGPTVNRKWGIKNENKHIFSVLGVSAGYQRNLSKSLSIIAEP
                     YLKLPMSGVGYGKVKLNSVGLLFSIAVKPFSVSKKINHFPDQ"
     gene            1359..2690
                     /locus_tag="PLO70_16565"
     CDS             1359..2690
                     /locus_tag="PLO70_16565"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76134.1"
                     /translation="MKYFFTLRFLTFFILSLIIVYSCSKNGRGTTAPDPCSGVTVTIS
                     GTVNNPTTIGASDGSIVASASGGSGFSYNINGGAFQSSSSFTGLAAGSYTIMSRNSNG
                     CTGTSIFVVVNPSNPCTAVTIVVTGTVTNPTTAAAANGSIIAAASGSTGFTFSINGGA
                     FQSTGNFTGLGAGAYSITAKDGNGCIGSANFTLTAPNPCTGLVITVTATLTNPTTVGG
                     NNGSIVASASGSTGFSYNINGGAFQASGVFSGLTAGSYAVSAKDINGCIGSGNFILTD
                     PDPCAGVTITVNNTITGNTPCQANNGMVTAVAAGGTGTYTYSLNGGVFQASNIFSNLG
                     PGSYTITAKDVNSCTGTSIPAIVSNLPEGPLFSQVKAVLQANCVPCHNNSQSEGGMNW
                     TVDCNIVINRDRIRARAVDANPSAMPPTGLLPASERQKIINWINAGGKFTD"
     gene            complement(2746..3303)
                     /locus_tag="PLO70_16570"
     CDS             complement(2746..3303)
                     /locus_tag="PLO70_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQZ76135.1"
                     /translation="MRKIVVAITGASGSIYAENLLKKLIAAKEQWDELSVVMTTNARE
                     VWKTELGNESWNDFAVKFYTTTDFSAPFASGSGQYDTMIIIPCSMGTLGRIATGVSND
                     LITRAADVVLKERRKLICVVRDTPYNLIHIRNMETVTLAGGIICPATPSFYSKPSTIE
                     EVAATVTDRVLDLAGLTIKTYRWGK"
BASE COUNT         1061 a          701 c          781 g         1059 t
ORIGIN      
        1 aggataagaa gagaaggttt atctttttct tattgttgtt tttgtttttg ggaggagcag
       61 gaataattat tggaaagcca ttccggccgg gtaagcaagt tgcccgtgct gaagaagcaa
      121 caggagaaag taaaagcagc aagccggcgc ataactcatt tgaaggaatg ggagcaggta
      181 ccacagatga aaagacacca acagggattg gtaagagtga agacatttat cccaaagctg
      241 tgaaggaaag tactgccgtt acagatgttg atatagatgc taactccgat tataacaata
      301 agaataatat tcttattgct gctaaaaaat atcctgccgg ccaaaccagg ctgaacggtt
      361 cagtaaatca acagaaaaaa aggcagtcgt atatgcagta tgataagcca attgacaatg
      421 cagctgtcac aagtgttgat atgcaacttg acaaacctgc taacaatatt gtgaaaccag
      481 ccgtggtggc tattacaaaa aatagtataa ccattgatgc tgccgataat aacaaagagg
      541 atgtggaaat caaagaaatg tctgtgaaag ctggcgctga aaagatagaa gtgtctgaca
      601 gtgtcgcaaa taaatccgct attaccataa agaaaacgca ggaaaaaaag aaagcaaatg
      661 ggaaggcaca aaaaagaaat tctttctttt taagcttctc tgctggtccg gatgtaagct
      721 atgcaagttc cggtaaaccg ggcaagatca gacctgtcgc tggccttggt cttggatata
      781 cttttaaaga caggcttact gtccgtaccg gcttttattc ggccagtaag gtctattcag
      841 catcgccgga agcctatcat ccaccggctg tattttattc ctattatccc tacctggaaa
      901 aagtggatgc agattgtaaa gtgtatgaat taccgctaac attcagctat agttttaatc
      961 ataccaaaca gcaacattgg tttgcatcag ctggcatttc ttcttacctg atgaaaacag
     1021 agacctataa ttatttttat aaaacttccc ccctggggcc aactgttaac agaaaatggg
     1081 gaataaaaaa tgaaaacaag catatctttt ctgtacttgg tgtctctgca ggatatcagc
     1141 gaaatctaag taagtccctt tccattattg cagaacctta tttaaaacta cctatgagtg
     1201 gagtgggata tggtaaagta aaattgaata gtgttggtct gctgttttca attgctgtaa
     1261 aaccattttc tgtttcaaaa aaaataaacc acttccctga tcagtaaaag aggcaacctg
     1321 tttttacaag actgcgattg agtattaaaa atcagcttgt gaaatatttt tttacactca
     1381 ggttcttaac ctttttcatt ctatcattaa ttattgtgta ttcctgcagc aagaatggtc
     1441 gaggaacaac agcacccgat ccctgcagtg gtgtgaccgt tacgatcagt ggaacagtta
     1501 ataatcccac aaccattgga gcaagtgatg gaagtattgt agcttccgca tcagggggta
     1561 gtggattctc ctacaatatt aacgggggag cttttcaatc ttcttcgagc tttacaggtc
     1621 ttgcagccgg ttcgtacact atcatgtcga ggaattcaaa tggttgcacg ggtacttcta
     1681 tatttgttgt cgtaaaccct tctaatccct gtacagctgt tacaattgtt gttacaggta
     1741 ccgtcacaaa cccaactact gcagcagctg cgaatggcag tattattgcc gcggcttcag
     1801 gcagtaccgg gttcaccttt agtatcaatg gaggcgcttt tcaatcaact ggtaatttta
     1861 ccggacttgg ggccggtgcc tattccatta ccgcgaaaga tggtaatggt tgtatcgggt
     1921 cagctaattt tactttaacg gctccaaatc cctgtaccgg gttggtaata acagtaacgg
     1981 ctacactaac aaatccaaca actgttggag ggaataacgg aagtattgtg gcatcagctt
     2041 ccggaagtac gggattctcg tataatatta atggcggtgc tttccaggct tcaggtgttt
     2101 ttagtggttt gacagccgga tcatatgctg tttctgcaaa ggatattaat gggtgtattg
     2161 gttccggcaa ctttatactg acagatcctg atccctgtgc cggagttacc attacagtta
     2221 ataacactat aacaggtaat acaccctgcc aggccaataa cgggatggta accgctgttg
     2281 ccgctggtgg aaccggtact tatacttata gtctgaatgg cggtgtgttc caggcatcaa
     2341 atatattttc aaatcttggc cccggcagtt atacaataac tgcaaaagat gttaatagtt
     2401 gtacagggac cagtatcccg gcaattgtaa gtaatcttcc tgagggccct ttgttctcac
     2461 aagtaaaagc tgtattgcag gctaattgtg taccctgtca taataattca caatcggaag
     2521 gaggaatgaa ctggaccgtt gattgcaata ttgtgataaa cagggacaga ataagagcaa
     2581 gggcagttga tgccaatcca tctgccatgc cacctacagg attactccct gcttcagaac
     2641 gtcagaaaat tattaactgg attaacgctg gtggtaagtt caccgattag ttggttactg
     2701 gtggtgtatg ttcaaagggg ttgcttttgt ccggcaatcc cttttttatt ttccccaccg
     2761 gtaagtttta atagtaagac ctgccaggtc aagtaccctg tctgtaactg tagccgctac
     2821 ttcttcaatg gtagacggtt tactataaaa tgatggcgtg gcagggcaga tgataccacc
     2881 ggccagtgtt actgtctcca tgttgcggat atgaataaga ttataaggcg tatcgcggac
     2941 tacacagatc agttttcttc tttctttcaa tactacatct gcagcccttg taataaggtc
     3001 atttgaaact cccgttgcta ttctgccgag tgtgcccatc gagcagggaa tgatgatcat
     3061 tgtatcatat tgaccggagc cggaagcaaa aggtgctgag aaatcagtag tggtataaaa
     3121 ttttacagca aaatcattcc atgattcatt accaagttct gtcttccaaa cttctctggc
     3181 attggtggtc attacaacag ataattcatc ccactgttct tttgcagcaa tcaatttttt
     3241 cagcaaattt tccgcatata tggaaccact ggccccggta atggcaacta caatttttct
     3301 cataacatgt ataactatgt aagactggat aaagttgcag gaagatagtc caatttatgc
     3361 aaacgtttgc tcttcggggc ttgtagttgt aaaactacaa gcttaatcac tggtgtattg
     3421 agtaaaatca ataattgatt atttggtgct gattcttgtc ataatagtca aaaaatgaca
     3481 gccaaatgtt ttgtgttatc ctattattaa cgatattagc attgaatttt cataggataa
     3541 ggtttaatgg ttaatggttt ttgattaggg ccccccgatt ctcgtcgggg gtcttttttt
     3601 gc
//
LOCUS       DAORTI010000234         3531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_35463_length_3531_cov_6.178078, whole genome shotgun sequence.
ACCESSION   DAORTI010000234 DAORTI010000000
VERSION     DAORTI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35463_length_3531_cov_6.178078"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1459
                     /locus_tag="PLO70_16575"
     CDS             230..1459
                     /locus_tag="PLO70_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQZ76136.1"
                     /translation="MLFEFNRFSSLLLIFFVHGLVYALLLFRKGIVNETRSDKWLSLF
                     LLLCILYIAPWMIGFGGWYDNQPYRDFLFYVPLQQLYFIGPVIFFYVQSLLNPSFRFG
                     KKEWLHLLPGILYLLFSIVMVVTDKLVLKKYFFLANGQDPDFDTWYQLTGFFSMLIYF
                     LLSLRYYSLYKKLMVQVISYADVVMFRWIQNFLLAFLLMLTVRLGFYISDYFIGSTYA
                     SSWWYFFVFAVIFYYIAITGYANSIETKIAFHPNLLTYRPALLLNYHPSHTNESPLTK
                     EEVEIDIDTTPVTINNETADIKEWKEKLLQLMQSGKAYEDPELSLAQVAKQLQSNPSF
                     ISRMVNTGFGVNFNDFVNQFRIEAVKEMLNNGEHKKQTLLGIAFDCGFNSKATFNRAF
                     KKVTGSSPKEWIEKNKR"
     gene            1505..1888
                     /locus_tag="PLO70_16580"
     CDS             1505..1888
                     /locus_tag="PLO70_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76137.1"
                     /translation="MKRVLLLLLVFYTMPSFGQDTIKKTSPDSLLGTWVINLRPAPDS
                     EPYLKDFKFTKIEGKQFDGEFYGYPFSGGLLNTDWDRIYFAFTTADQSGTYFHSGYIE
                     NNKVFGITLNENRKFVLPWKGQKKN"
     gene            2039..2389
                     /locus_tag="PLO70_16585"
     CDS             2039..2389
                     /locus_tag="PLO70_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76138.1"
                     /translation="MKKAMIILAAAITFSSCSLSSRTTIGPQKAFELGDGKHGSFKAV
                     VKNDSDVPVEVYKVPPGGAETKLITLQPGQQKTVRFDADTKAIFKNTGNKTAAVLLKV
                     TGDTGLTMGGPNYN"
     gene            2416..3372
                     /locus_tag="PLO70_16590"
     CDS             2416..3372
                     /locus_tag="PLO70_16590"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQZ76139.1"
                     /translation="MKQLLHQFFFDTSLNDNKVLNAGWLLFRLHIGLSIAMHAGWPKM
                     NTISAPGWFTEQVAGLGFTFPSPGFWAAMASWGEFAGGICIALGFLTRFSALQLVFQF
                     FVIAFLWYDKPEPLTGMYFQQTLFWGFTLVMFAGGGRYSLDRLIMNRKKAKIPMPAKA
                     AIACLLLLVTTGANAQTLSGSDFKPLEGKWTGKLTYLDYTSDTPVTIKVNAAVEMKSE
                     NQFTLSFYYTDEPAENEKKNYVINGNGTRLNDMHLIERALQTEGALKIVLERKDRDGN
                     DHKPATIHQVIVIAENFFTITKLVKFDGEEQFFQRHQYSFSR"
BASE COUNT         1016 a          692 c          728 g         1095 t
ORIGIN      
        1 atgccaacga agacatattg tttgtataaa tatttttacg ctgtgctgat ctcctttaga
       61 aacctgctca ccctctgccg tcaatttaag tggtacctgt aattttttaa ctgttcggac
      121 aaccttgtct tgtacaagac caacatcact tgctaaacta atacactgca gaaccgttat
      181 tagtacaaat aagtaaccgc attagccggg gttttaatat tttacataaa tgttattcga
      241 atttaatcgg ttcagttccc tgttgctgat cttctttgtg catggattgg tgtatgccct
      301 gttattattc aggaagggaa tagtcaatga aacccgttct gataaatggc tcagtctttt
      361 tttactgctc tgtatcttat acatcgctcc ctggatgatt ggttttggcg ggtggtatga
      421 taaccagcct taccgtgatt ttttattcta tgttccattg cagcagttat attttatagg
      481 cccggtcatt ttcttttatg tgcaaagcct gctaaacccc tcattccggt tcgggaaaaa
      541 agaatggctg catttattac cgggtatact gtatttgctg ttcagtatcg taatggtggt
      601 gacagacaaa ctggtgctaa agaaatattt tttcctggca aacggacaag accctgattt
      661 tgatacctgg taccagctta ccggtttctt ttctatgctg atctatttct tattatcctt
      721 acgctattac agcctgtata aaaaactgat ggtgcaggtc atcagttatg cggatgttgt
      781 tatgttccgc tggatacaaa attttctgct ggctttttta ttaatgctca cggtgcgact
      841 tggcttttat atcagtgatt attttatcgg cagcacttat gccagtagct ggtggtattt
      901 tttcgtattt gctgttatct tctattacat cgctattacc ggctatgcca attctattga
      961 aactaaaatt gcttttcacc cgaacctgtt aacctaccgg ccggccttgt tgctgaatta
     1021 tcatccttct cataccaacg aaagcccgtt aacgaaagag gaagtggaaa ttgatattga
     1081 tacaacgcct gtcaccatca ataacgaaac agctgatata aaagaatgga aggagaaatt
     1141 attacaattg atgcaatcgg gcaaagcata tgaagacccg gaactgtctc ttgcacaggt
     1201 ggcgaaacaa ttgcaaagca acccctcttt catttccaga atggtaaata ctggttttgg
     1261 cgtaaacttt aatgactttg taaaccagtt ccggattgaa gccgtaaaag agatgctcaa
     1321 taatggggag cataaaaaac aaacactgct ggggatcgct tttgattgcg ggtttaactc
     1381 taaagccacg tttaaccggg cttttaaaaa agtgaccggc tcaagcccaa aggaatggat
     1441 agaaaagaat aaacggtaga accatttacc ggatgatttg tttccctggt ttaaatcaat
     1501 caatatgaaa agggttctgc ttcttctcct tgttttttat acaatgccat cttttggcca
     1561 ggatacaata aagaaaacca gccctgatag tctgttaggt acctgggtta ttaacctcag
     1621 gcctgcgccg gacagtgagc cttacctgaa agatttcaaa ttcactaaga tcgaagggaa
     1681 acaattcgat ggagagtttt atggctatcc cttcagcggg ggattattga atacggattg
     1741 ggatagaatt tatttcgctt ttacgacggc tgatcaaagc ggcacttatt ttcattcggg
     1801 ctatattgaa aacaataaag tttttggtat caccctcaat gagaacagga aatttgtgct
     1861 tccctggaaa ggacaaaaga aaaattaatc cgttgtttcg gatagataaa atcaatttga
     1921 gccgtctcaa atcataaatg aggcggtttt tttatttgaa accccatttt ttagtctcaa
     1981 aacatctttt gcagcgactg cggcctttca tgctctcatc tttgaagaaa taaaaaacat
     2041 gaaaaaggca atgatcatcc tcgcagcagc aataaccttc agctcctgtt ctcttagctc
     2101 cagaactact attggtcctc aaaaagcctt tgagctgggc gatggtaaac atggttcgtt
     2161 taaagccgta gttaaaaacg atagtgatgt tccggtagag gtttacaaag ttcctcccgg
     2221 aggggctgaa accaaactga tcaccttaca acccggccag cagaaaacag taaggtttga
     2281 tgctgacacc aaagctattt ttaaaaatac cggcaataaa acagcggcag tattgctgaa
     2341 agtaactggt gatacagggc ttactatggg tggaccgaat tataattgat aaaccatttt
     2401 aaccaatact tttttatgaa acagctactc catcaatttt ttttcgacac cagcctgaat
     2461 gataataagg ttttaaatgc tggctggctt ttatttcgtc tgcatattgg tttaagtata
     2521 gccatgcatg caggctggcc caaaatgaat acgatttcgg cacccggctg gtttacggaa
     2581 caggtggccg ggcttgggtt tacatttcct tctcccggat tctgggcggc catggcttcc
     2641 tggggtgagt ttgcaggtgg catttgtatt gcgttggggt ttcttacccg attctctgct
     2701 ttgcaattgg tctttcagtt ttttgtgatc gcctttttat ggtatgataa gccggaacct
     2761 cttacaggga tgtattttca gcaaacatta ttctggggat ttacattggt aatgtttgcg
     2821 ggcggtggca ggtattcctt agacaggtta attatgaaca ggaaaaaagc aaagattccc
     2881 atgcctgcta aagcagctat tgcctgcctt ttattattgg taacaaccgg ggctaacgca
     2941 caaaccctaa gtggtagcga ctttaaacca ttggaaggaa agtggacggg taagcttact
     3001 taccttgatt atacatcgga tacaccggta actattaaag ttaatgctgc cgttgaaatg
     3061 aaaagcgaaa atcagttcac cctgtctttt tattatacag acgaaccagc ggagaatgag
     3121 aaaaaaaact atgtaattaa cggaaatggt accaggctaa acgatatgca tttaattgaa
     3181 agggctttac aaacagaggg agcattaaaa attgtattgg agcgtaaaga ccgggatggt
     3241 aatgaccaca aacccgccac cattcaccag gtgatagtta ttgctgagaa ttttttcact
     3301 ataaccaaac tggtaaaatt tgatggtgaa gaacaattct ttcaacggca ccaatattct
     3361 tttagccggt aatagacgaa tcgcattaag aagaggcgtt aattataact agttctgatt
     3421 atctcttttg tatatgcgat gtattaatga atacaggtca cttcacctct tcaaatacac
     3481 cggtctgttt attcttccgc acattgtctg cagtaaaata acggccattc a
//
LOCUS       DAORTI010000235         3523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_35594_length_3523_cov_5.745386, whole genome shotgun sequence.
ACCESSION   DAORTI010000235 DAORTI010000000
VERSION     DAORTI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35594_length_3523_cov_5.745386"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLO70_16595"
     CDS             <1..1049
                     /locus_tag="PLO70_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQZ76140.1"
                     /translation="PSLKEVFKNDFAIGTALNTEQIEEKDPRAAALVPQQFNMATPEN
                     IMKAEIIHPQWGSYNFDLADKMIAFGEKHNIKINGHTLIWHSQLPAFAHNIQSVDSFR
                     TFFTDHINTVAGRYSGKLFSWDVVNEALNEDGTMRKSIFLDKLGDNFVTEAFRLAQAA
                     SPATELYYNDYNNEQPAKRAGCITLIKKVQAAGVRIDGVGIQGHWHVGKVPLKDIEES
                     ILQYSELGLKVMFTELDIEVLPRNFQGADISQRMKNDPSLNPYVNGLPDSVQQQLAKD
                     YQALFNLFLKHKDKITRVTFWGVNDGQSWLNDWPVRGRTNYPLLFDRQFMPKPAFYKV
                     IETKKDFENIKKGF"
     gene            1069..2520
                     /locus_tag="PLO70_16600"
     CDS             1069..2520
                     /locus_tag="PLO70_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ76141.1"
                     /translation="MSNSTSQKLSVFEKVGYSLGDLAANLVFQTLVTYLAYFYTDIYG
                     LKANDASAIILTVGLIAAFGFNPIIGAIADRTVSRWGKFRPWILVTAIPLGVIALLAF
                     TTPDFSYKGKMIYAVVTYTLLLLLYAANNLPYSALSGVITGDMKERNSMSAYRFVAVM
                     FAQFFVQVFMYAIIKSAGGGNRPEQMAVGMEKVFTWLSITGTIMLIITFLTTKERIVP
                     KPEQKSTLGQDLKDLGKNKPWVIMLVLTLLVFITLAMKGGSYVYYFENYVDKESLAKL
                     ISPVLNGFSNAGINFFGEDPVSAGLGLFNAGGIICMIIGITLSKPLADKYGKRDVFGI
                     ALVVSTLFIVLFFFFPPQSVSLMFISQILHGFFYGITIPLLWAMIADVADFSEWKNNR
                     RATAIIFSAMMIGLKAGLTIGSSLLTWLLGLYDYIPNNTTGQTETAIQGTKMLVSIFP
                     AIPFLVGAALLFFYEINKKMEVQIESDLKARRN"
     gene            2567..>3523
                     /locus_tag="PLO70_16605"
     CDS             2567..>3523
                     /locus_tag="PLO70_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQZ76142.1"
                     /translation="MPEDLINHIDFENLNQRAISQPLVKHIYIADPSAHVFNGKIYIY
                     PSHDIDTGDAFDDLGSHFAMEDYHVISMDTPQGEAVDNGLALHVKDVLWAEKQMWAPD
                     ATEKDGNYYLFFPAKGYDGIFRIGVAISDSPVGPFTPQPEAIKDSFSIDPALFKDDDG
                     SYYMYFGGIWGGQLQRWRTGTFQAEQPESPFAFLPEANEPALCPKVAKMASNLLEFAE
                     APRDLVILDENNQPLLQGDTNRRFFEASWMHKYNGKYYFSYSTGDTHFLCYAIGNNPF
                     GPFRYAGRVLEPVVGWTSHHSICEFNGKWYLFYHDSSLSKGIT"
BASE COUNT         1057 a          700 c          734 g         1032 t
ORIGIN      
        1 taccttcttt aaaagaagtg tttaaaaatg attttgctat cggcactgcc ttaaatacag
       61 agcagatcga agaaaaagat ccccgggcag cagcactggt tccgcaacaa ttcaatatgg
      121 caaccccgga gaatatcatg aaggctgaaa tcattcaccc gcagtggggc tcctacaatt
      181 ttgaccttgc tgacaaaatg attgcattcg gggaaaagca taatataaag atcaacggac
      241 atacgctgat ctggcatagc cagttgcctg cctttgccca caacatccaa agtgttgact
      301 ccttcagaac cttcttcacc gatcatatta acacggttgc cggccgatac agcggaaagt
      361 tattctcctg ggatgtggta aacgaggcat tgaatgaaga tggtacgatg cgtaaatcta
      421 ttttccttga taaactggga gataattttg ttactgaagc atttcgttta gcacaagcgg
      481 cttcacctgc tacagagctc tattacaacg actacaacaa tgaacaaccg gctaaacggg
      541 caggctgcat tacactgatc aaaaaagtgc aggcagcggg tgttcgtatt gatggtgtgg
      601 gtatacaggg tcactggcat gttggtaaag tcccgttgaa agatattgaa gaaagtatcc
      661 tgcaatattc cgaacttggt ttaaaagtga tgtttacaga actggatatt gaagtgctgc
      721 cccgcaattt ccagggcgct gatataagcc agcggatgaa aaatgatccg tcactcaatc
      781 catatgtaaa cgggctgccg gatagtgtac aacaacaatt agccaaagat taccaggccc
      841 tgtttaatct atttttaaaa cataaggata aaataaccag ggtaacattc tggggagtga
      901 atgatggtca gagctggctg aatgattggc ccgtcagagg cagaactaac tacccgttat
      961 tatttgacag gcaattcatg cctaaacctg ctttctacaa agtaatagaa acaaaaaagg
     1021 attttgaaaa tatcaaaaaa gggttttgat ttttaaacta atagtaacat gagcaacagc
     1081 acatcccaaa aattgtcggt atttgaaaaa gtaggttaca gcttaggtga ccttgcggca
     1141 aacctggtat ttcaaaccct tgttacttac cttgcttact tctataccga tatatatggg
     1201 ctcaaggcaa atgacgcttc tgccatcatc ttaacagttg gcctaattgc tgcatttgga
     1261 tttaacccca tcattggtgc tattgcagac agaactgtat ccaggtgggg taaattcaga
     1321 ccctggatac tagttacagc aataccatta ggggttattg cgctattggc attcactact
     1381 cccgattttt cctataaagg aaaaatgatt tatgcagtag ttacttacac actgctgtta
     1441 ttactttatg cagcaaacaa ccttccctat tctgctctga gtggggtaat aaccggcgat
     1501 atgaaagaac gaaacagtat gtctgcctat cgttttgtag cggtgatgtt tgcgcagttt
     1561 tttgtacagg tattcatgta cgctataata aaatctgccg gtggtggtaa caggcctgaa
     1621 caaatggctg ttggaatgga aaaggtattt acctggcttt ctataacagg caccattatg
     1681 ctgattatca cttttttgac aacaaaggaa aggattgtcc caaaaccgga acaaaagtct
     1741 actcttggac aggatttgaa ggatcttggc aaaaataagc cctgggttat catgctggta
     1801 cttactctcc tggtctttat aacactggcc atgaaaggcg gatcttatgt ttattatttt
     1861 gaaaactatg ttgacaagga aagtctcgct aaactgatca gtccagtact gaacggattt
     1921 tccaacgcag gcatcaattt ttttggtgaa gaccctgttt ccgcaggcct gggtttattt
     1981 aatgcaggcg gaattatttg tatgatcatc ggcattacgc tttcaaaacc acttgctgat
     2041 aaatatggca aaagagatgt ttttggaata gcattagtag tatcaacatt attcattgtt
     2101 ctctttttct tcttccctcc gcaatcggta agtctaatgt ttatctcgca aatcttacat
     2161 ggattctttt atggcatcac tataccctta ttatgggcaa tgattgctga tgtagctgat
     2221 ttcagtgaat ggaaaaataa ccgtagggca acagctatca ttttttccgc catgatgatt
     2281 ggattaaaag ctggtttaac tatcggcagt tcattactca cctggctgct gggattatat
     2341 gactatatac caaataatac cacgggacaa acagaaacgg ctatacaagg cactaaaatg
     2401 ctcgtaagca tttttcctgc tatccctttt ctggttggtg cagcactatt gtttttttat
     2461 gagatcaata aaaaaatgga agtgcagata gaaagcgact taaaagcaag gcgaaactga
     2521 ttttttatgt aaacttaatt tgtagtatta aaaccaaata actgcgatgc ctgaagatct
     2581 gattaatcat attgactttg aaaatctgaa tcaacgtgcc atatcgcaac cattggtaaa
     2641 acatatctac attgccgacc cttctgcaca tgtgtttaat gggaaaattt atatttatcc
     2701 ctcacacgat atagatactg gtgatgcatt tgatgacctg ggtagccatt ttgccatgga
     2761 agattatcat gtaatttcta tggacactcc gcaaggcgaa gcagttgata atggtttggc
     2821 acttcatgtt aaagatgtgc tatgggcaga aaagcaaatg tgggcacctg atgccacaga
     2881 gaaagatggc aattattatt tattctttcc tgcaaaagga tacgatggca ttttccgtat
     2941 tggcgttgct atcagtgact ctccagttgg cccttttact ccacagcccg aagctattaa
     3001 agacagtttt tctatagacc ctgccctatt caaagatgat gacggtagtt attatatgta
     3061 cttcggcggt atctggggcg gccagttaca gcgatggaga accggaactt ttcaggctga
     3121 acaaccagaa agtccttttg catttttgcc ggaagctaac gaaccggcat tatgccccaa
     3181 agttgcaaaa atggcaagta acttattaga atttgcggaa gcgccgagag acttagtcat
     3241 actcgatgaa aataatcaac cccttttgca gggagatact aacagaagat tctttgaagc
     3301 cagctggatg cataagtata acggcaaata ttacttctcc tactccaccg gtgatactca
     3361 tttcctatgt tatgccattg gcaataatcc attcggccca ttccggtatg caggaagagt
     3421 tttagaacca gttgttgggt ggacatccca tcattccatt tgtgaattca atggcaaatg
     3481 gtacttattt tatcatgatt ccagcttatc aaaaggcatt acg
//
LOCUS       DAORTI010000236         3504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_35936_length_3504_cov_5.715570, whole genome shotgun sequence.
ACCESSION   DAORTI010000236 DAORTI010000000
VERSION     DAORTI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35936_length_3504_cov_5.715570"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..2677)
                     /locus_tag="PLO70_16610"
     CDS             complement(116..2677)
                     /locus_tag="PLO70_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQZ76143.1"
                     /translation="MKRTLTALVLLMQSFVSNSQLIVETNATANSFMLGNPFICVDTN
                     DESLVQLAAGFLQNDIKAVTGAIPVLTGSLPDKAETVIIIGSLKSSPLLRSLIKSNKI
                     KTDHINGKWEAYSIQTIENPFKGIAHALVITGSDRRGTAYGVFELSKQIGVSPWYWWA
                     DVPVKKKEAVYIKKNSIHSDLPKVKYRGIFINDEAPALSNWSKEKFGGFNHHFYEKVF
                     ELILRLKGNYIWPAMWGNAFYYDDSLNIKSADEYGIVIGTSHHEPLMRAHEEWKYYGK
                     GRKWNYDSTETGLKEFWKGGMERAWNEKIVSVGMRGDGDEPMSRETATALLERIVKDQ
                     RQIIEEVTQKPASETPQLWALYKEVQDYYDKGMRVPDDITLLLCDDNWGNIRKLPKLT
                     DPPRKGGYGIYYHFDYVGGPRNYKWVNTNPLPRIWEQMHLAWEYNARQIWIVNVGDIK
                     PMEFPISFFLDYAWNPEKIGADDLQIYAEYWSASQFGSTHAKEIADILAKYAKYNGRR
                     KPELLDANTYSFNYNEWSTVVNDYKSLLKKAEEINKQLPAEYKDAYFQLVLHPVKACA
                     NLNEMYYHVALNKEAYKNKWEEANGYADKVKQLYENDSLITLEYHQLNKGKWNHMMSQ
                     THIGYTYWQQPNKQKMPAVNYVPADSILTGIDTTSSWKEALYPKSLAKNIFYEQNGYI
                     SIEANHFTKAVNTNDITWKSLPDHGRTGSAITSFPVTANQQKPGGKAAHLQYEIYTYS
                     EDSVTLYCYFSPTLNFHNAEEGLQYAISIDNETPQIFSINKEDKNSISGIWNKWVAES
                     IIIKSSKHKLLQPGKHTVKYWVVSPAVVLQKIVLDFGGVKRSYLGPPETLYQKKK"
     gene            complement(2691..3461)
                     /locus_tag="PLO70_16615"
     CDS             complement(2691..3461)
                     /locus_tag="PLO70_16615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQZ76144.1"
                     /translation="MNAGNSPLGIGDKKVAIVTGGGSGLGFAIAERFTHNGILTIIAG
                     RDKEKLENAKTQLGELCHAIPCDVSKLASIPGFVADVINQFGHIDILVNNAGINMKKE
                     FTEVTDEDFQRIITTNLSAVFAMSREVVKEMLKKGSGSIINISSMAAQYGLPKVIAYS
                     ASKTAIDGLTRAMAVELSPKGIRVNAIAPGFIYSEMTAKALDSDPERKAKVFSRTPMG
                     IMGQPVDIGEAAYFLASDAAKYITGVVLPVDGGNSIGF"
BASE COUNT          870 a          742 c          665 g         1227 t
ORIGIN      
        1 ttcttttaaa gaaggtattg attgtgcgga tgagtttttt tgactgcctg cgcaagagac
       61 cagcagtaaa ctgacgccgc atagtgccgg aataaataat cggtttatca ttattttatt
      121 ttttcttttg gtataatgtt tcaggtggcc cgagataact tcgtttgacg ccgccaaaat
      181 ccaatactat tttttgtaat accactgccg ggctcaccac ccaatatttt accgtatgct
      241 taccgggttg cagtagttta tgtttgcttg atttaatgat tatactttct gccacccatt
      301 tgttccagat gccgctgatg ctgttcttat cttctttatt gatactgaaa atttgcggtg
      361 tttcattatc aattgaaatt gcatattgca aaccttcttc tgcattgtga aaatttaagg
      421 taggagagaa gtaacaatat agtgtcacac tgtcttcact gtaagtataa atctcatatt
      481 gaagatgtgc tgctttgcca ccgggttttt gttgattggc cgtaactgga aaacttgtga
      541 tggcggatcc ggttctgcca tgatcaggta aagacttcca ggtgatgtca tttgtgttaa
      601 cagcttttgt aaaatggtta gcttcgatag agatatagcc attttgttcg tagaagatat
      661 tttttgccag gctctttggg tacagtgctt ctttccaaga tgatgttgta tctattcctg
      721 tgagaataga gtcggcaggt acatagttta cagctggcat tttctgcttg tttggttgtt
      781 gccaataagt atatccgata tgtgtctggc tcatcatgtg attccacttg cctttgttta
      841 attgatggta ttcaagtgtt atcaatgaat cattttcata aagctgtttc actttatcag
      901 catagccgtt agcttcttcc catttatttt tataggcttc tttgttcagg gcaacatggt
      961 aatacatttc gttcaggttg gcgcaggctt tcaccgggtg taagaccaat tgaaagtagg
     1021 cgtctttata ttccgcaggt agttgtttat ttatttcctc tgcttttttt aacaggcttt
     1081 tatagtcatt tactactgtg ctccattcat tgtaattaaa actataagtg tttgcatcaa
     1141 gaagctcagg ttttcttctt ccgttatatt ttgcatattt agcaaggata tcagcaattt
     1201 ctttggcgtg ggtacttcca aattgtgaag cactccaata ttctgcatat atctgcagat
     1261 catctgcacc aattttttct gggttccagg catagtctaa aaagaaggag atgggaaatt
     1321 ccatgggttt gatatcacca acattcacga tccagatctg tcttgcatta tattcccatg
     1381 ccaggtgcat ttgttcccat attctaggaa gcggatttgt atttacccat ttataatttc
     1441 gggggccacc cacataatca aaatgatagt aaatgccata cccgcctttc ctgggcggat
     1501 cagtaagttt tggcaatttt ctgatgttac cccagttatc atcacataaa agcagggtga
     1561 tatcatccgg cacccgcata cctttatcat agtagtcttg cacttctttg tataaggccc
     1621 agagttgtgg cgtttcgctg gcaggttttt gtgttacttc ttcaatgatc tgccgttgat
     1681 cttttacaat tctttccagc agggcggttg ctgtttctct tgacatcggc tcatctccat
     1741 cacctctcat ccccacactt acgatttttt cattccatgc tctttccatt ccccctttcc
     1801 agaattcttt caggcctgtt tctgtactgt catagttcca tttgcgtccc tttccatagt
     1861 atttccattc ttcatgtgcc cgcatcaaag gctcatgatg cgatgtgccg atcacaattc
     1921 cgtactcatc ggctgatttt atattcaaag aatcgtcata ataaaatgca ttaccccaca
     1981 tggcaggcca tatataattc cctttcagtc tcaggatcag ttcaaatact ttttcataaa
     2041 agtgatgatt aaagcctccg aatttttctt tgctccagtt agaaagagca ggcgcttcgt
     2101 cattaataaa aatgccccgg tattttactt taggtaaatc gctgtggata gaatttttct
     2161 taatataaac tgcttctttc ttttttaccg gcacatcggc ccaccaatac cagggtgata
     2221 caccaatttg tttggacaat tcaaatacac catacgcagt tcctcttctg tcgctgcctg
     2281 ttattacaag ggcatgggca atacctttga aagggttctc tattgtctga atgctgtagg
     2341 cttcccattt cccattaata tggtccgtct ttatcttatt gctttttata agtgatcgaa
     2401 gaagtggtga actttttaaa gaaccgatta ttatgacagt ctcagctttg tctggtaatg
     2461 agccggtaag taccgggatt gctcctgtaa ctgcttttat atcattttgc aagaacccgg
     2521 cagctaattg tacgagggac tcatcattgg tatcaacaca tatgaacggg ttaccaagca
     2581 taaaggaatt ggcagttgca tttgtttcaa caatcagctg actgtttgaa acaaagcttt
     2641 gcatcagcaa taccagagct gttagtgttc ttttcatata gttaatcgtt tcaaaaaccg
     2701 atgctattcc ccccatccac agggagtaca acaccggtta tatatttcgc agcatcactg
     2761 gcaaggaaat aagctgcttc gccaatatca actggttgtc ccataatgcc cattggtgtt
     2821 cggctgaata cttttgcttt tctttcggga tcactatcga gtgcctttgc tgtcatttct
     2881 gagtaaataa atccaggggc aatagcattt acacggatac ctttgggaga taattcaaca
     2941 gccatcgctc tggttaatcc gtcaattgct gttttgctcg cactgtacgc aattacttta
     3001 ggcaacccgt attgtgctgc catggaactg atgttgatga tagagcctga tccctttttc
     3061 agcatctctt ttactacttc cctgctcatg gcaaatacgg cagaaagatt ggtggtgatg
     3121 atccgttgaa aatcctcatc agtaacttcc gtaaattctt ttttcatatt gatcccggca
     3181 ttgtttacca gtatatctat atgaccgaat tgattgatca catcagcaac aaacccgggt
     3241 atagaggcca gtttactaac atcgcaaggt atggcatggc acagttctcc caactgtgtt
     3301 ttggcatttt ctagtttctc tttatctctt cctgcaatga tggttaggat accattatgg
     3361 gtgaaccgtt ctgcaatggc aaaacccaat cccgaacctc ctcctgttac tattgccact
     3421 tttttgtccc cgatccctaa aggggagttt ccagcattca taatttgtaa tttcttttat
     3481 taataaaaaa tttcatttgt tgta
//
LOCUS       DAORTI010000237         3475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_36397_length_3475_cov_4.713450, whole genome shotgun sequence.
ACCESSION   DAORTI010000237 DAORTI010000000
VERSION     DAORTI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36397_length_3475_cov_4.713450"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLO70_16620"
     CDS             complement(<1..455)
                     /locus_tag="PLO70_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76145.1"
                     /translation="MKKPVFQLLAATAIIAIGLTLVQLKWTTTKETHSYQEKEEEEEG
                     DELQSGAAMQLRGWFQAKAYPNPENLSAKYQKAWEEYKEIKKKTQNQHATRLTSASNW
                     TQLGYSQNGSTRIGGRIVCMAIDPNNTNNLWVGSASGGIWRSTNAGTSWA"
     gene            complement(527..3163)
                     /locus_tag="PLO70_16625"
     CDS             complement(527..3163)
                     /locus_tag="PLO70_16625"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ76146.1"
                     /translation="MKKHFFLLIASAICLFSSVDKAVAQCPGGYTQAQLNWDNLDYYF
                     NNGLNVRPYGYGSPNGTFISDAMEQTQKFAIGTNWLSIVTSSNALINPGNFNGVTGEN
                     AIHTGNIAGYAGDDVQYNPSANGQTITITFNTQVANASFTLYDVDGASRIDFAAQNSI
                     AIPQVINMAFQGGSTLTPFLNNTITPYVVGAAANDANNINNSTVTVTVAGPVKQITIS
                     CTTMGTDPVFWLSDINACVTGSFPPNYQQSFNTRPFYGPVISQPDYFLVTPDNNSCYM
                     QDPATGTTRWLFTDASNTYMNSFAYDPGNRILYYISEGLSLNRNNKTLKKYDFNTETI
                     STVVADIVTTLGIPTFNGGVESGGAAYYDDKLYIGIEGGQYNSTNTKESMIWCIEFDA
                     SLNPNNAYQVYSTNTYSGSNIIHDWADFLIKNGVLYNYNSAKTSTPTYPNSSYTHIDL
                     MTGVATKYINPTPANPYAGQAGMSWNGNQYSIRNVIQQYNEDGTLGVSVPITVVGGGP
                     AWVANAGDASENFRPKCDFGDAPATYDPYADPATQSPAVHERSELIRLGATWDNEFWK
                     RGTAGTDDTDDGLAYTPIMAPGGGGYVVQVAAFNNSGADATMIAWLDYNGNGVFDAAE
                     AITPITVPTSASSQNFWLYWPVTTNTFSNGDFTYLRIRITAASAGMTTSHPTGYFTNG
                     EVEDYRILVDNFPLANHLLNFEASLQDKKVKLNWEVSEDAGLYAYEIERSRDNINWTK
                     ITTVNANGTAGTYNYESIDAQPLKGISYYRLRLIESTGMNRFSLVKRITIKDLESGLI
                     VAPNPAKDNITIRFDAAGGGDIEISILNMQGSMQLTRKQRITAGLNNIMISLPETMSA
                     GTYVARIVVGGEVVYKKLIVNK"
BASE COUNT          991 a          722 c          662 g         1100 t
ORIGIN      
        1 gcccagcttg taccggcatt ggttgatctc cagataccac cactggcaga acccacccac
       61 aaattattgg tgttattggg gtcaattgcc atacaaacaa ttcttccccc gattcttgta
      121 ctgccattct gactatagcc caactgtgtc cagttactgg cactggttaa ccgggtagca
      181 tgctggtttt gtgttttctt ttttatttct ttgtattctt cccaggcttt ttgatacttg
      241 gcactcaggt tctccggatt aggataagcc tttgcctgga accatccccg gagctgcatg
      301 gcagcaccac tttggagttc atctccttct tcctcttctt ctttctcctg gtaactatga
      361 gtttcttttg tagtagtcca ttttaactga actaatgtga ggccgatggc aatgattgct
      421 gtagcggcca ataactggaa cactggtttt ttcatacaaa attggatttg gtagggttat
      481 tagtatgaca gaggttatgt tgtaatcact gtcagtaaat actattttat ttatttacaa
      541 ttaacttttt ataaaccact tctccgccaa ctactatacg ggctacataa gtaccggcac
      601 tcatcgtttc gggaagtgag atcattatat tatttaatcc ggcagttatt cgctgctttc
      661 ttgttagctg catactaccc tgcatattca ggatactgat ttcgatatcg ccgcctccgg
      721 ctgcatcaaa acgaatagta atgttatctt ttgcaggatt aggtgcaacg atcaaaccgg
      781 attcaagatc tttgatagtg atgcgtttaa ccaggctgaa cctgttcatc cctgtacttt
      841 caattaaccg caggcggtaa taagatatgc ctttcaaagg ttgtgcatct attgattcat
      901 aattatatgt acccgctgta ccatttgcat taaccgttgt aattttagtc cagttgatat
      961 tgtcccggct tctttctatc tcgtaagcat acaggccggc atcttcagat acttcccagt
     1021 tcaattttac tttcttatcc tgaagacttg cttcaaagtt taataaatgg ttagccaggg
     1081 gaaaattatc tactaatatc ctgtaatctt ctacctcacc attagtaaaa taaccggtcg
     1141 gatgcgaagt tgtcatacct gccgacgctg ctgtaatacg gatgcgcagg taggtaaagt
     1201 caccatttga aaaagtattg gtagtaacag gccagtataa ccagaaattt tgagaggatg
     1261 cggatgtcgg tacggtaatg ggtgtaatgg cttctgcggc atcaaaaacg ccgttgccat
     1321 tataatctaa ccaggcaatc atagttgcat ccgctccgct gttattaaat gcagcaacct
     1381 gcaccacata gccgccgcca cccggtgcca taatgggtgt ataggccaat ccatcatctg
     1441 tatcatctgt tccggcagta cctcttttcc aaaattcatt gtcccaggtg gctccgagcc
     1501 ttatcagttc tgatctttca tgtactgccg ggctttgtgt agccgggtcg gcgtaaggat
     1561 cataagttgc tggtgcatct ccaaaatcgc atttaggtcg gaaattttct gaagcatcgc
     1621 ccgcattagc cacccatgca gggcctccgc caactaccgt tattggaact gataccccaa
     1681 gtgttccatc ttcattatat tgttgaataa cgtttcttat tgaatattga ttcccgttcc
     1741 agctcatacc cgcctgacct gcataagggt ttgctggagt agggtttata tattttgtag
     1801 ctaccccagt catcaggtca atgtgggtat atgaactatt aggataagtt ggtgtactcg
     1861 ttttggcaga gttatagtta taaagtacac catttttgat taaaaagtct gcccaatcat
     1921 gtataatatt actgcctgaa tatgtgtttg tactgtaaac ctggtatgca ttattaggat
     1981 taagtgaagc atcaaactct atacaccata tcatggactc cttcgtatta gtactgttat
     2041 actgtcctcc ttctatacct atgtataact tatcatcgta ataagctgcg ccacctgatt
     2101 ctacacctcc attaaatgtt ggtataccga gtgtagttac aatatctgcc actacagtag
     2161 agatagtttc tgtgttaaag tcgtattttt ttaaagtttt attatttcta tttagagaaa
     2221 gtccctcaga aatataataa agaattctat ttcccggatc gtaggcaaac gaattcatat
     2281 aagtattact ggcatctgta aaaagccatc ttgttgttcc tgtcgcagga tcctgcatat
     2341 agcaagaatt attatctggt gttaccagaa agtaatctgg ctggctgatg acagggccat
     2401 agaaaggccg ggtattaaaa ctttgctgat agtttggtgg aaaactaccg gtaacacaag
     2461 cattgatatc cgataaccaa aatacaggat cggtacccat ggtcgtacaa gaaattgtta
     2521 tttgttttac aggaccggct actgttacag tcacagtgct attattaata ttgttagcat
     2581 catttgcagc tgcaccaacc acatatggtg ttattgtatt atttagaaaa ggagtaagag
     2641 tagaaccacc ttgaaatgcc atattgatta cttgaggaat agctatggaa ttctgagcag
     2701 caaaatctat tcttgaagcg ccatctacat catataatgt aaatgaagca ttagcaacct
     2761 gagtattaaa agtaatcgtt attgtctggc cattggctga tggattatac tgtacatcat
     2821 ctcctgcata acccgcaata tttccagtat gaattgcatt ttctccagta accccattaa
     2881 agttaccagg attaattaat gcatttgaac tggtaacaat agacagccag ttggtaccaa
     2941 ttgcaaactt ctgagtctgt tccatagcat cagagatgaa agtaccattg ggagagccgt
     3001 acccataagg acgaacattg agcccgttat taaaataata atccaggtta tcccagttca
     3061 attgtgcctg tgtatatcca ccggggcatt gcgcaacagc tttatcaaca gaagaaaata
     3121 aacaaattgc agaagcaata agcagaaaga agtgcttttt cattagacag agtttggaca
     3181 atggtttttt atatattgga ttctacaaat agaaataata atcattaacc gctgatcaat
     3241 aaaacactac tgcctttatt attatttctc atgctaaagt gagagtattt acttagaaat
     3301 acaacgcttt cgtaaagttt caggaataat aaagtacaag tacaaaccat cctcgtaaat
     3361 catacaaaga cccatggtat ttctacgaaa aagaccccaa aagcggctca tgataaatca
     3421 ctatttttac tgataatctg aacggtatga gtcaacataa accagtaatt ggcat
//
LOCUS       DAORTI010000238         3468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_36519_length_3468_cov_6.489599, whole genome shotgun sequence.
ACCESSION   DAORTI010000238 DAORTI010000000
VERSION     DAORTI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36519_length_3468_cov_6.489599"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLO70_16630"
     CDS             complement(<1..803)
                     /locus_tag="PLO70_16630"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQZ76147.1"
                     /translation="MATRNSPNTTKVSNKFFGEGLTFDDVLLMPGYSQVLPRDVEIKT
                     RLTKNITLNVPLLSAAMDTVTEAALATALAREGGLGILHKNMTIEKQAEQVRKVKRSE
                     SGLIIDPVTLLADATIGDALKLMRENKIGGIPILDKSGKLVGILTNRDLRFEEDMKRK
                     VSEVMTKENLYTAPEGTDLKKAEQLFKKTKVEKLPIVNKQGKLTGLFTYSDILKLKSN
                     PNAVKDKFGRLLVGAGVGITTDILDRVAALQTVGADVIALDSAHGHSKGV"
     gene            complement(849..2837)
                     /locus_tag="PLO70_16635"
     CDS             complement(849..2837)
                     /locus_tag="PLO70_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ76148.1"
                     /translation="MSHNVVAYSQACTTLGQTPSTAFPVCGTTVFHQASVPTCSTNDL
                     FVPGCSGAGAADYQNKNPFFYKFTCYVGGTLGFVITPLTSNEDYDWQLYDITGRNSND
                     IFTVNSLVVTGNWSGSYGPTGASASGVNFIQCASDPTENKPRFAAMPVLTAGREYLLM
                     VSHFTDSQSGYDLAFGGGTAVITDPKVPQMLSAKVDCDGKVITLKLNKQIRCSSATGS
                     GSEFSLSPAAGTVVSAVSANCTGAFDFNEMTITLAAALPNGTFDLVINNGTDGNSLLD
                     ICGTAITPGDKVSFTYAVPQPIFADSIGKTACTPDSVRIYFPKKISCASISADGSDFV
                     VAGPTPVTVIGATGNCVNGKSDYVVVKFAAPIFTKGIYQLTLKAGNDGTVLIDECGLE
                     TPVHTLPFTTADTVNAAFTYTEKLGCRTDTLSFSHNGAHDVNSWNWIFNNGTPITSQA
                     HTIVFSATSNNTIQLRVSNGTCSDTSSTQINLDNEVKADFNMPSIICPEDKLEVFNTS
                     TGLIDSWKWTFDVIATSTLKDPLPVQFPIINREAYYTVKLVAKSNALRCSDSARRTLT
                     VLDHCLIAVPTAFTPNNDGRNDFFRPHNALKADSYEFKVYNRWGQLVFQTNNWQDKWD
                     GKINGNLQGTGVFVWMLRYIHRDTKKEVFEKGTVTLIR"
     gene            complement(2950..>3468)
                     /locus_tag="PLO70_16640"
     CDS             complement(2950..>3468)
                     /locus_tag="PLO70_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQZ76149.1"
                     /translation="GGGLLTEFRSKSKPDKHNFPSRNRIVAGMSDATIVIETSIKGGS
                     MITAELANGYNKDVFAIPGKITDSKSAGCNYLIKSNKAVLLPDTNEFLQLMGWEDAVK
                     NKKKQQREIFIELTKEEKIVIDILKENETVSIDEINFKSGLSSSTVAAAILNLELQNV
                     ILSLPGKLYKIA"
BASE COUNT          958 a          700 c          663 g         1147 t
ORIGIN      
        1 actcctttgc tgtgtccatg tgcactgtct aatgcaataa catcagcacc tacagtttgt
       61 aatgcagcaa ctctgtctag tatatcagta gtgatgccaa caccggcacc aactaatagg
      121 cgaccaaatt tgtctttaac tgcgttagga ttgcttttta gtttcaatat atcgctatat
      181 gtaaacagcc cggttagttt gccttgctta tttactatcg gaagcttttc aacttttgtt
      241 ttcttgaata attgttctgc ttttttcagg tctgtgcctt cgggagctgt atatagattc
      301 tcttttgtca ttacttcact tacctttctc ttcatgtctt cttcgaagcg gagatcccta
      361 ttggtaagaa tgccaaccag ttttccactc ttatcaagaa taggaatgcc gccaattttg
      421 ttttccctca ttagttttaa tgcatcacca atggtagcgt ctgctaataa tgtaacagga
      481 tcgatgatca atccgctttc acttcttttt actttgcgaa cctgctcggc ttgcttttct
      541 atagtcatgt tcttgtgcag tattccgagt ccaccttctc ttgccaaagc tgttgccagt
      601 gctgcttcgg taacggtgtc cattgctgca gagagcaagg gtacattcaa cgttatgttt
      661 ttagtgagtc ttgtttttat ttccacatct cgtggaagca cctggctgta gccgggcatt
      721 aataaaacat catcgaaggt taggccttcg ccgaaaaatt tgtttgaaac cttagttgtg
      781 ttgggggaat ttcttgttgc catgtgcaaa gataagtggc ccggcaatta cagacaaact
      841 gaaatttctt agcgtattaa tgtgacagtt cctttctcaa aaacttcttt ttttgtgtcc
      901 ctgtgaatgt acctgagcat ccatacaaat acaccagtgc cttgcagatt accattaatc
      961 ttgccatccc atttgtcctg ccaattgttg gtttgaaata ctaattgccc ccagcgattg
     1021 tacactttga attcgtagct atctgctttc aaggcattat gcggtctgaa gaaatcattt
     1081 cggccgtcat tattgggagt aaaagcagta ggtacagcaa taagacaatg atcaagcaca
     1141 gttaaagttc tgcgggcact atcgctgcat ctcaatgcat tactcttggc aacaagcttt
     1201 accgtataat atgcttctct gttaattatc ggaaactgta caggtaaggg atcctttaat
     1261 gtacttgttg caattacatc aaatgtccat ttccaagagt ctattaatcc agttgaggtg
     1321 ttaaacactt ccagtttgtc ttcggggcaa ataatagatg gcatgttgaa atcagctttt
     1381 acttcgttgt ccagattgat ctgcgttgat gatgtatcac tacaagtacc gttacttaca
     1441 cgaagttgaa ttgtgttgtt actggtagct gaaaaaacaa tagtatgtgc ttgtgaagtt
     1501 ataggtgttc cgttattaaa tatccagttc cagctattta catcatgagc tccgttgtgt
     1561 gaaaatgaaa gggtgtctgt acgacagcca agtttttccg tataagtaaa tgcggcattt
     1621 acagtatcag cagtagtaaa gggtaatgta tgaacaggag tttccaatcc acattcatca
     1681 atcaacaccg taccgtcatt gcccgccttc agtgttaact ggtaaattcc ttttgtgaaa
     1741 atgggtgctg caaattttac tacgacataa tctgattttc cattaacaca atttccagtt
     1801 gcaccgataa ctgttacagg tgttggtccg gctacaacaa aatcactacc atcggctgaa
     1861 atgcttgcgc aacttatttt ctttggaaaa taaatacgga ctgaatctgg agtgcaggca
     1921 gtttttccaa tgctatcggc aaagatgggt tgaggtacag cataagtaaa ggaaacttta
     1981 tcacctggag ttatggctgt tccacaaata tctaataatg aatttccatc tgttccatta
     2041 ttgattacca ggtcgaaggt tccattggga agtgctgctg ctaatgtaat cgtcatttca
     2101 ttaaagtcaa aagcaccagt acagtttgct gaaacggcac ttacaactgt accggcagca
     2161 ggtgacaaac taaattcact tccagaacct gtggcactgc tgcatcttat ctgcttattc
     2221 agtttcagtg taattacttt accgtcacaa tcaacttttg cgcttagcat ttgtggaact
     2281 tttggatcgg tgataacagc agtgccgcca ccgaatgcta aatcataacc gctttggcta
     2341 tctgtgaaat ggctaaccat taaaaggtat tcccggccag cagttaatac aggcattgcc
     2401 gcaaacctgg gcttattctc tgtcggatca gatgcacatt gaataaaatt aaccccactt
     2461 gcagaagcac cggttggtcc ataagagcca gaccagttac cagtaacaac taaagaattt
     2521 acggtgaaaa tatcattgga atttcttccg gttatatcat acagctgcca gtcataatct
     2581 tcatttgagg ttaatggtgt tattacaaaa cccagtgtac cgccaacata gcaagtaaat
     2641 ttataaaaga aagggttttt attttgataa tcggcggcac ctgcacctga acagccggga
     2701 acaaataagt cattggtact acaggtaggt actgatgcct gatgaaaaac ggtggtgcca
     2761 caaaccggaa aagcggtgga aggcgtttgc cccaatgtgg tacatgcttg tgaataagca
     2821 acaacattat gggacaaaaa aatgatgatt aataatatga agggtatgcg ccgcaatttc
     2881 ataagtaaaa ggtcaatgaa ttcttgaatt actttaagac aataaaacct ggttttttgc
     2941 tgtatggttt taagctatct tatataattt tccgggcaaa gatagtatca cattctgtaa
     3001 ttctaaatta agtatggcgg ctgcaacagt actgctgctt aatccacttt taaaattgat
     3061 ttcatcaatt gatacggttt cattttcttt gagaatatca atgacaattt tttcttcttt
     3121 tgtcaattca ataaagattt ctctttgttg tttcttttta ttttttactg catcttccca
     3181 acccattagt tgtaagaatt cattggtatc aggtaataat actgctttat tggatttgat
     3241 gaggtaattg catccagcac ttttactgtc agtaattttt ccaggaatag cgaaaacatc
     3301 tttgttgtaa ccattagcta attcagccgt aatcatgctg cctcctttaa tacttgtttc
     3361 aattacgatt gttgcatcac tcatacctgc tacaattcta tttcggctgg gaaaattatg
     3421 tttatccggt tttgatttgc ttctgaattc tgttagcaat ccccctcc
//
LOCUS       DAORTI010000239         3412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_37498_length_3412_cov_5.205541, whole genome shotgun sequence.
ACCESSION   DAORTI010000239 DAORTI010000000
VERSION     DAORTI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37498_length_3412_cov_5.205541"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLO70_16645"
     CDS             <1..716
                     /locus_tag="PLO70_16645"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQZ76150.1"
                     /translation="EYFYLRIAGEIYSSISRNSGYFDEDAGHNLWAIGLLLLTPIMLY
                     HIILELTMNGQSLGKKVLGLRVVDEHGGKASISQYLIRWLLRLSDLWIVMILFYIASN
                     PGFNNAETIFVVLFGLGFLITDVVLVVSSKKGQRIGDILAKTILIRTHSKGNIEDTVF
                     IDVADSYVPSFPQIMQLSDKDINAIKSILETSRKKGDYNMAESASSKVKAHLKIESGL
                     PPFDFLDVLLKDYNYLSTK"
     gene            complement(1359..1811)
                     /locus_tag="PLO70_16650"
     CDS             complement(1359..1811)
                     /locus_tag="PLO70_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76151.1"
                     /translation="MENSTSDKSLFDLSFDENVKQSLKGAATWGGLAAIVSLIGSILG
                     LVNYFVEKGKESRYGGNSLEEMQIQQAANTGGFVSVVITLIIGIILFYFLNKFSRSAK
                     AGIDTNDHYLINEGLGSLSTYFKFIGVLLIIILVLFALGILIGIGQGV"
     gene            complement(1832..2254)
                     /locus_tag="PLO70_16655"
     CDS             complement(1832..2254)
                     /locus_tag="PLO70_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76152.1"
                     /translation="MEQNQDQSLFDMNMDSSTQSHLLSISKWTKFISIVGFVICGLVL
                     LLLAVFGAKITEQLSVLFSFGNSELAGALIAVVLVVLLLFGFWIYFLFRASNLLRNGL
                     QSRDTAQLADGFKAMRIYFIFSFVISVLSLLSTLQSFL"
     gene            complement(2355..>3412)
                     /locus_tag="PLO70_16660"
     CDS             complement(2355..>3412)
                     /locus_tag="PLO70_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ76153.1"
                     /translation="DLPLLINQWANVVRWEMRTRLFLRTAEFLWQEGHTAHATKDEAI
                     EETVKMLHVYADFVENWMALPVLKGVKTANERFAGAEDTYCIEALMQDGKALQAGTSH
                     FLGQNFAKAFDVKFSDKENKLEYVWATSWGVSTRLIGALVMAHSDDQGLILPPRIAPF
                     QVVIVPIYKGDEQKAAIDVKVNEIIKELKALGIRVKYDNNDNARPGWKFAEYEMKGVP
                     VRLAIGARDLEKNVVEIARRDTKEKHVVSIDGLAEYINGLLSAIQQNMFNTAKTYLDE
                     HITNADSWDEFVKLLDEKGGFIAAHWDGTSETEEAIKEKTKATIRCIPLDNKTEEGQC
                     ILTGKSSSQRVLFARAY"
BASE COUNT         1087 a          744 c          576 g         1005 t
ORIGIN      
        1 ttgaatattt ttacctgcgg attgccggag aaatatacag ttctatcagc agaaactccg
       61 gctactttga cgaagacgcc ggtcataatt tatgggcaat aggattattg ctgctcactc
      121 ccattatgct ctaccatatt atacttgaac taacgatgaa tggacaaagc cttggcaaga
      181 aagtattggg acttcgggta gtagacgaac atggcgggaa agccagcatc agtcagtatt
      241 taattcgctg gctgctgcga ttgtcagatc tgtggatcgt aatgatactt ttctatatcg
      301 cttcgaaccc gggttttaat aatgccgaaa cgatctttgt tgtattattt ggattaggct
      361 ttctgattac cgatgttgtg ctggttgtat cttccaaaaa agggcagcgc ataggcgata
      421 tccttgccaa aacaattctt atccgcaccc attcaaaagg caatattgaa gacaccgttt
      481 ttatagatgt ggcagacagt tatgtgccat ctttcccgca aataatgcaa ttaagcgaca
      541 aagatatcaa tgcaattaaa agtatactgg aaacctcccg caagaaagga gattataata
      601 tggcagaatc tgcctcctca aaagtaaaag ctcacctgaa aatagaatcc ggcttacctc
      661 catttgattt cctggatgta ctgctgaaag attataatta tttgtcaaca aaataagaga
      721 tcggcaaggg ttaaaacgca ggtttgaagc cggagatcat cccgaaaaat cacaatgtca
      781 ttattgcagc aaagccaccc aataccaatg caagaataaa aaatacccct gcaatgaccg
      841 ttaagactcc catatacctg aaagtaattt taagattttt caacccttca ttaaagagac
      901 ctgatcattc gcaattattg catttttgga atgatttgca aaccgcagtg taaaaaagca
      961 gggggaaaaa taataagtgc taataagagg caagttattt ccattgttat cccaaatcca
     1021 tccggtgtgc ccgacttgtc aaagttatta aaaccggcat ctaatccgaa tagctgttga
     1081 gacatataaa atgaaaacac gaatccaaca atcgccatta atacacatat taccattcct
     1141 gcaatggcag gaaactctgc ccgggaggcg gtttctttca agtggttttc gctatagtat
     1201 ccacatttaa tccaaacaat atcatatcct gatactgaac tataaacttg atttttaaaa
     1261 ttggattaca aaaaatattg ccttgcacta atcataaaag gcatgcctac ccttatatac
     1321 aggatgtgaa ttagaaaaat agtatttatt gactgctgtt aaactccctg gcctattcca
     1381 atcaaaatac ccaaagcaaa caatacgagt attataatca gtaatacacc aatgaattta
     1441 aaataagttg acaagctacc cagtccttca ttaatcaggt aatggtcgtt tgtatctatc
     1501 ccggcctttg cagacctgga gaatttattg agaaaataaa aaagtattat gccgataatt
     1561 aaagtgatca caacagaaac aaacccacct gtattagcag cttgttgtat ctgcatttct
     1621 tcgaggctat ttcctccata cctgctttcc tttccctttt caacaaaata attaacaagg
     1681 ccaagaatag atccaattaa tgaaacaatt gcagctaacc caccccaggt ggcagctcct
     1741 ttaaggcttt gctttacatt ttcatcaaaa ctgagatcga aaagtgattt atctgaagtt
     1801 gaattttcca ttttagtaaa agtttagggt attataaaaa agattgcaat gtagacagga
     1861 gactcagaac tgaaatgaca aagctgaaga taaaatatat cctcatagct ttgaaaccat
     1921 cggccagttg agcagtgtcg cggctctgca aaccatttcg taacaaattg gaagccctga
     1981 acaaaaaata aatccaaaaa ccaaataaca ataaaacgac cagcactact gcaatgagtg
     2041 ccccggccag ctcactattt ccaaacgaga ataatacgga gagctgttca gtaatttttg
     2101 ccccgaaaac agcaagaagc aataatacca atccacaaat aacaaagccg acaatagaga
     2161 tgaattttgt ccacttgctg atagaaagta aatgactttg tgtactactg tccatgttca
     2221 tatcgaacag ggactggtcc tggttttgct ccataaagct gttgattttg atttacataa
     2281 atataaaact ccatatatta aactgcaaat ctttttgaag attttaatca ggaattaaaa
     2341 tatcaccatt gaatttaata tgccctggca aacaatactc tttgtgagga tgatttaccg
     2401 gtaagaatac attgaccttc ttcggtctta ttatcaagtg gaatgcagcg gattgttgct
     2461 tttgttttct cttttatagc ttcttctgtt tcagaagtgc catcccaatg cgcagcaata
     2521 aatccgcctt tttcatccag taattttacg aactcatccc agctatcggc attagtaata
     2581 tgctcatcca agtatgtctt tgcagtattg aacatatttt gttgaatagc agacaacaga
     2641 ccatttatat attcagccag tccatcaatg cttaccacat gcttttcctt tgtatcacgt
     2701 cgggcaattt ccacaacatt cttttccagg tccctggcac caattgccaa tctcaccggc
     2761 actcccttca tttcatattc tgcaaacttc cagcctggtc ttgcattatc gttgttatca
     2821 tatttaaccc tgatacccaa agcttttaat tctttgataa tttcattcac tttaacatct
     2881 atggcagctt tctgctcatc acctttataa atcggaacga taaccacctg gaaaggtgca
     2941 attctgggtg gcaggatcaa tccctggtca tcactatgcg ccattaccag cgcaccaatt
     3001 aatcttgtac ttacgcccca gctggtagcc cagacatact ccagtttatt ttctttatca
     3061 ctgaatttaa catcaaaagc tttggcaaaa ttctggccca aaaaatgtga agtaccggcc
     3121 tgtaatgcct ttccatcctg cattaatgct tcgatgcaat aagtatcttc agcaccggca
     3181 aaacgttcat tagccgtctt tactcctttt agcacaggca atgccatcca gttctctaca
     3241 aaatctgcat acacatgcag cattttcact gtttcctcta ttgcttcgtc ttttgtagca
     3301 tgagcagtat gtccttcctg ccataaaaat tcagcggtac gcaaaaacaa tcttgtcctc
     3361 atttcccagc gaaccacatt ggcccactgg ttaatcagaa gcggtaaatc ac
//
LOCUS       DAORTI010000240         3406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_37597_length_3406_cov_4.748135, whole genome shotgun sequence.
ACCESSION   DAORTI010000240 DAORTI010000000
VERSION     DAORTI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37597_length_3406_cov_4.748135"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3406)
                     /locus_tag="PLO70_16665"
     CDS             complement(<1..>3406)
                     /locus_tag="PLO70_16665"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQZ76154.1"
                     /translation="KNFIAKTPTSTLRADAVVYPVNVSANNSRQSSSIEISNSFISQQ
                     DIRLLANDISRQYASQLNALGDLAITANMSGTPQEMKIRELKLRSVNGRTFTADLSGS
                     LANITNPKNLGYNLNIRNLTAERSLIAPYIQQSGQKIQLPPIINLSGTLAGNTNSVQT
                     NVTINSAFGKVTAKGMLVNFQQPAALTYDMVISATALETGKWVYQDSLLGKLTGTINA
                     KGNNGFNIKKNNLDLTANINSFRIQKNTYNNIDLDAYFEKGLVEMKANIKDPVLNALL
                     NGNADIRNEYPTLHADIDLPAANLYALGFTPDSMVVSTIGRVDINNADPSSLDAVIRL
                     DSILLKRNGSNIYADSILLTGKVRNDSTFFTLNSPNAGADIASNLTYIEMPELLNEVM
                     SYYMPPPGTTAAKQAPPGSITASILLYPDETYRILVNDLDFRNIQAHAFITNQQPDSA
                     VKATIVGEKLTASGVTLANLNTVISGTKDSLLMTLTADTLASKGILLYEGLVKAGFAD
                     NNFIADVETKDSKQKKQYAFAVTGKQNTTANGYDIKLGDNLMLNYDQWNINPQNLVSV
                     TGKGFNIKNFDINNSNQRLALSSTGSGYNDPLKVDITNFELSTITTAYNQDSTQLRGL
                     INSNFIVSNFGQTIPTVDGLLQIDSLFYQQAPLGNLQLNASANNDKVQLDGKVDGFGN
                     NVTIAGDYSANNIDLDINMSPLKLLTLQPFTAGNLAGSKGDISGPIKIKGPVNNPVWN
                     GELQFNGIETITTQFGTFLRIDGQKITLAYPSINFDNFIVNDSTGNALKIDGNLLQNE
                     SKNFISDLYLKTDQFNIMNSTAMDNSMLYGKAIVSLEGNLKGVVTSPEFIGGAVIKNG
                     TRITYVQENLAESLREREGVIEFVDMDTISNLLMKKTAQDVAAEKKSFTANKGLFTYN
                     INLEVEREAEFIVIVDPVTRDELKVKGTGNINMSVNPNGSISMAGIYKLNSGSYELNY
                     GIVNRKFILLDGSTVMLSGDPNNASLDITAAYEISTPPIDLISNEIGGSTSPASVPYK
                     RKIPFEVLLKITGTALKPLLAFDIRIKDKAEGVSYALETTIENKLEQLRTDESQMNKQ
                     VFALLALNRFIGDESSNFFGGSSIGSSGSFLANESVSAFLNDAINQI"
BASE COUNT          951 a          660 c          638 g         1157 t
ORIGIN      
        1 aatctgatta atagcatcat ttaaaaaagc actgacactt tcattggcca ggaagctgcc
       61 ggaacttcca atactggatc ctccaaagaa attactactc tcatctccaa taaagcgatt
      121 aagagccagt aaggcaaaaa cctgtttatt catttgcgat tcatcggtcc ttagctgttc
      181 aagtttgttt tcaattgtgg tttccaaggc ataactcaca ccttctgctt tatcttttat
      241 ccttatatca aaagctaata aaggttttaa ggcagtacct gttattttta ataacacctc
      301 aaaaggaatt tttcttttgt agggaacact tgcaggtgaa gtggaaccgc cgatttcatt
      361 actgatcaga tcaattggcg gagtgctgat ctcatatgca gctgtaatgt ctaggctagc
      421 attgttgggg tcaccggaga gcataaccgt acttccgtca agtaaaataa attttcggtt
      481 tactattcca taattcaatt cataactgcc actgtttagt ttataaatac cagccataga
      541 gatagagcca ttcggattta cactcatgtt gatattgcca gtgcctttta cttttaattc
      601 atcccgggta acagggtcta caataacaat aaattctgct tctctttcta cttccagatt
      661 aatattatag gtgaataaac ccttattagc ggtaaacgat ttcttttctg ctgcaacatc
      721 ctgcgctgtt tttttcataa gcagattact gatagtatcc atatctacaa attcaatcac
      781 tccttcccgt tctcttaatg attcagccag attttcctgt acgtatgtta tacgggtgcc
      841 atttttgatt actgcccctc ctataaattc aggacttgtt acgactccct taagatttcc
      901 ttctaaactt acaatcgctt ttccatatag catgctatta tccatagcag tactgttcat
      961 tatattgaac tgatcagtct tcaggtataa atcgctgata aaatttttgc tttcattttg
     1021 caggagatta ccatcaatct tcaatgcatt gcctgtgctg tcgtttacaa taaaattatc
     1081 aaagttgatg gatgggtatg ctaaagtaat tttctgtcca tcgatacgta aaaaagtacc
     1141 gaattgtgtt gttatggttt ctatgccatt gaactgaagt tctccattcc aaacaggatt
     1201 attaacgggc cctttaattt ttatcggtcc ggaaatatct cctttacttc ctgccaaatt
     1261 accagcagta aatggttgga gtgtaagtaa tttcagcgga ctcatattaa tgtctaaatc
     1321 aatattatta gctgaatagt cgccggctat tgttacatta tttccaaagc catctacctt
     1381 gccatctaat tgtaccttat cattattggc agaagcgttc aactgtaaat ttccaagtgg
     1441 tgcctgttga taaaataggc tgtcaatctg gagcaggcca tcaacggttg gaatcgtttg
     1501 tccaaaatta ctgacgatga aattagaatt aatcaaccca cgcagttgtg tagagtcctg
     1561 attataggca gtcgtaatag tgcttagttc gaaattagtg atatctactt ttagcggatc
     1621 gttgtaaccc gacccagtac tactgagggc taaacgctgg ttactattat taatgtcgaa
     1681 gtttttgatg ttaaaacctt ttcccgttac acttaccagg ttttgcggat ttatattcca
     1741 ttgatcataa ttcagcatca ggttatcacc gagtttaata tcatagccat tagcagtagt
     1801 attctgcttt cctgtaacag caaaagcata ttgttttttt tgtttcgaat cttttgtttc
     1861 tacatcggca ataaaattat tatcggcaaa accagctttt accaggcctt catacaataa
     1921 aattcctttg gaagcaaggg tgtcagcagt aagtgtcatg agcaaagaat ccttggtgcc
     1981 gctaataact gtattgaggt tagccaaagt aacaccgctg gccgttaatt tttctcctac
     2041 aatagtagct ttaacggcac tatctggttg ctgattagtt atgaatgcat gagcttgtat
     2101 atttctgaaa tcaagatcgt ttactaatat ccgataggtt tcatccgggt aaagtaaaat
     2161 gctggcagtg atgcttccgg gtggagcttg ttttgcagca gtagtaccgg gtggcggcat
     2221 ataataactc atcacttcat tgagcaattc cggcatttct atataggtaa gattgctggc
     2281 aatatcagca ccagcgttgg gactatttaa cgtaaagaaa gtagagtcat tccgaacttt
     2341 tccagtaagc agtatgctat ctgcataaat atttgacccg tttcttttaa gtagaatact
     2401 gtctaaacga ataacggcat ccaggctgct tggatcagca ttattgatgt ctacacgacc
     2461 aatagtactg acaaccatac tgtcgggtgt gaaacctaag gcatataaat ttgcagctgg
     2521 cagatcaata tcggcatgca atgttggata ttcattgcgg atgtctgcat tgccatttag
     2581 cagcgcattc aatacggggt cttttatatt ggccttcatt tccactaagc ccttttcaaa
     2641 atatgcatcg agatctatat tgttgtaagt atttttttgt attctgaatg aattgatatt
     2701 tgctgtcagg tcgagattgt ttttcttaat gttaaaccca ttgtttcctt ttgcattaat
     2761 agttccggtt agtttaccca gcaatgaatc ctgataaacc catttgccgg tttccagtgc
     2821 ggtggcacta atcaccatat catatgttaa tgcagcgggt tgttgaaagt ttaccaacat
     2881 tccttttgcc gtgacttttc caaaagctga attgatggta acattcgttt gtacactgtt
     2941 cgtattacct gcaagagtac ccgaaagatt tataattggg ggcaactgta ttttttgtcc
     3001 gctttgttgt atatagggag caatcaatga ccgttctgct gtaagattcc ggatgttgag
     3061 attataacca agattttttg ggtttgtaat attagcaagg gatcccgaaa gatcggcagt
     3121 aaacgttcgt ccgttgactg atcgtaattt taattcccgg attttcattt cctgtggagt
     3181 gccagacata ttggccgtta ttgccagatc tcccaatgca ttcaattgcg aagcatattg
     3241 acgactgata tcattggcta aaagacgaat atcttgttgc gagataaagg aatttgaaat
     3301 ttcaatagaa ctgctttgtc tgctgttatt agcagaaaca ttcacgggat atacaactgc
     3361 atctgcccgc aatgtagagg taggtgtttt tgctataaaa tttttt
//
LOCUS       DAORTI010000241         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_38307_length_3367_cov_6.500604, whole genome shotgun sequence.
ACCESSION   DAORTI010000241 DAORTI010000000
VERSION     DAORTI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38307_length_3367_cov_6.500604"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1395
                     /locus_tag="PLO70_16670"
     CDS             292..1395
                     /locus_tag="PLO70_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76155.1"
                     /translation="MKNCLLLYLLLLPAFILKAQIQGPLNGNSFTTVAIPGSNQTWVN
                     PGNVSASDNIYSTFGNITAGVGSYTDYLVATNFGFSIPSGVTINGIVVEVERSDPNFR
                     TADYRVRIVKGGTIGAAERASGSSYPSSDSYQSYGNAGDLWGESWTQADINATDFGVA
                     IAAQRSVNGGTTGGRVDHIRIIVFYDFTTLPVTLINFSATKKDRSVDLRWTTADETNM
                     SHYEVERSTNARDFLPVQYVTSLDQNRETNYIAEDTDPVSGISYYRLKMVENDGRVKY
                     SKIISMQFRTGNAITLYPTVWKNGAILQITNPEYEKLVIYFSNATGQVTGKTVTNSTI
                     VPTSILSSAKGILFFSITDESGKLLGTGKLLAN"
     gene            complement(1537..2256)
                     /locus_tag="PLO70_16675"
     CDS             complement(1537..2256)
                     /locus_tag="PLO70_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76156.1"
                     /translation="MKKYIFCCLVLFSFFSLLAQNVGVNTSNPDPSAALDISSTNSGL
                     LVPRMNTGQRTTIASPANGLMVFDTDTKSYWFRESTNWVELASSIGLKSINGNATFTP
                     YNINPKRYIWELNGSHPTGTSIALPQAIIEDLCADEDGCKVTLTMTNWGAGITESASF
                     SGTFFYTNAGGTRRWRASNDISGIDGDNTVNHIFSIFSNFYFTDATYAVNVGTDPAVG
                     LHFMKWNAYPVSAIGRLIIED"
     gene            complement(2413..2907)
                     /locus_tag="PLO70_16680"
     CDS             complement(2413..2907)
                     /locus_tag="PLO70_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76157.1"
                     /translation="MAKIPLTKDSEFVVGEEKVLNYIMTAFFIALFLYGLIDAIRRHF
                     INIDYQSFIFGFALVPAIYCFRRAHSNRVYVRVNRTGIYKDEQLVTGWSNLLNAFITQ
                     KEKTGIFNIVDNFILVVEYTRDGSPEGFRRKIPLTNTQNKSEEEVLEAVKFFWSVYRK
                     DAGK"
     gene            3107..>3367
                     /locus_tag="PLO70_16685"
     CDS             3107..>3367
                     /locus_tag="PLO70_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76158.1"
                     /translation="MKKGILFLLLVSTLTSNAQSLKDALYGGKLKTDSGTVIKKGDDL
                     SSKIDTSKKKPVEPEKTKITAITRDSSITKMTIHTDSAAIVVN"
BASE COUNT         1048 a          686 c          643 g          990 t
ORIGIN      
        1 ttttgctcag ggcagggatt tgatttatcg ggatgattgc atccgcttgc aagtgcactg
       61 aactaaccag gttatcttca tcggtaaagg cctggtatat gctataaaga gtaagctggc
      121 aaactctgaa attaattgaa tcagcaaaga tttaggatat caacaagcca aggttccggc
      181 aatgcatacc taaatgctgc atttttaggt attcttattt ttcccaaaaa ttgatttatt
      241 tcttaacttg tttgaattat gtacttgccc cattcataat taaccaccag catgaagaat
      301 tgcttactct tatacctgct tttattacca gcttttattt taaaagctca gatacagggg
      361 ccattaaatg ggaacagttt tactacagtc gcaattcccg gatcaaatca aacctgggtt
      421 aaccctggaa atgtatcagc cagtgataat atttattcga cttttggcaa tataactgca
      481 ggtgttggca gttatactga ttacctggta gccacaaatt tcggctttag tattccttcg
      541 ggtgttacga tcaacggtat cgttgtagaa gtggaaagat ctgatcccaa tttccgtacc
      601 gccgattaca gggtaagaat tgtaaaagga ggaaccattg gtgctgcaga acgggcaagc
      661 gggtcaagtt atccatcctc cgactcctat cagtcgtatg gaaatgcagg cgatttatgg
      721 ggtgaaagct ggactcaggc tgatattaat gcaactgatt ttggggtagc tattgcagct
      781 caacgaagtg taaacggagg cactaccggc ggaagggtgg atcatatccg gataattgtt
      841 ttttacgatt ttactacttt gccggtgact cttattaatt tttcagcaac taagaaagat
      901 cgttcagtag acctccgttg gacaacagca gatgagacaa acatgagcca ttacgaagtt
      961 gaacgttcca ccaatgcccg tgatttttta cccgttcaat atgtaaccag cttagatcaa
     1021 aacagggaaa ctaactacat cgccgaagat acagatcctg tcagcggaat atcttattac
     1081 agactgaaaa tggttgaaaa tgatggcagg gtaaaatact ctaaaatcat ttcaatgcaa
     1141 ttccggacgg ggaatgccat taccctttac cctactgttt ggaaaaacgg cgcaatatta
     1201 caaatcacca atccggaata tgaaaaacta gtaatttatt tttcgaatgc taccggacaa
     1261 gttaccggga agactgttac aaattcaaca atcgtcccaa ccagtatact ttcatctgct
     1321 aaaggaattc tttttttcag tattactgat gagtcaggta aactcctcgg aacaggaaaa
     1381 ctattagcaa attgattaca cctctggcca atctgccatt caaataacta ttgcaattat
     1441 attctggtat ttttagaatc cggcagaact gcattttctc tttacccaat gcagtgtata
     1501 aattttaaaa agatctttag tctaaaatca atctcatcag tcttcaataa tgagcctgcc
     1561 aatagcagaa actggatacg cattccattt cataaaatga agaccgaccg ctggatctgt
     1621 acccacatta acagcataag tggcatcagt aaaatagaag ttagaaaata ttgaaaaaat
     1681 atgattgaca gtattatcac catcgatacc agaaatgtca ttactggccc gccatcttct
     1741 ggtcccaccc gcatttgtat agaagaaagt accgctgaag gaggcgcttt cagtaatacc
     1801 tgccccccag ttggtcatcg taagggttac tttgcagcca tcttcatcag cacaaagatc
     1861 ttctatgatg gcctggggca aagcgatgga agtaccagta ggatgtgagc cattcaattc
     1921 ccagatgtac cttttcggat tgatattgta aggcgtgaaa gtagcattgc catttatgga
     1981 tttcaacccg atactgctgg ccaattccac ccagttggta ctttcacgga accaatacga
     2041 ttttgtatca gtgtcaaata ccatcaggcc gttggcaggg cttgcaatag tagtgcgttg
     2101 accggtattc atcctgggaa ctaataagcc gctgttagtg gaacttatat ccagtgccgc
     2161 actggggtcg gggttactcg tattaacccc aacattttgt gctaataaag aaaaaaatga
     2221 aaacaatacc aggcagcaaa aaatatattt tttcatactt aatttattaa acgctgaaag
     2281 aaatttttag aaaaataaat atactatttt agttttaaat tctggcttac ctgacttgaa
     2341 ctgaactgga tattgcattt gtaatattca aaccaggtat tctaaacatc ccaggtaaaa
     2401 tacattgcgt ttttatttac cggcatcctt cctgtatact gaccaaaaaa atttcacggc
     2461 ttccagcact tcctcttccg acttgttctg ggtattggtc agcggtattt tgcgccggaa
     2521 accttccggg gaaccatccc ttgtatattc cacaacaaga ataaaattgt caacaatgtt
     2581 gaaaatacct gtcttttctt tctgggtgat aaaggcatta agaagatttg accaaccggt
     2641 taccaattgt tcatctttgt atatacctgt tctattgacc cggacataca ccctgttact
     2701 gtgtgccctt cgaaagcaat aaattgccgg caccagggca aatccaaaaa taaaactctg
     2761 gtaatcgatg tttataaaat ggcgccttat tgcgtcaatt aaaccataga gaaaaagggc
     2821 gataaaaaaa gctgtcataa tataattcag cactttctcc tcccccacta caaactcact
     2881 gtcttttgtt aacggaattt tcgccataat ctgttattat ttatgctgcc atcagcccaa
     2941 taaagcaggc tggtgaattt accatttctt ttgttttcca gccgggctgt attttttatg
     3001 ctgttaaaag ccgggaggaa gattatgaag atgctaacat ctttgctgct taacttgcat
     3061 agtgaaacac ctgaacaaga aattggcttt taaaatgaat gtttttatga aaaagggaat
     3121 attatttcta ttgctagtat ctacccttac ctccaatgca caatcactga aagatgctct
     3181 ttatggcggg aaactgaaaa cagattcagg cactgtgatc aaaaaaggtg acgacctgag
     3241 ttcaaaaatt gataccagta aaaagaaacc ggttgaaccg gagaaaacta agataacagc
     3301 gataaccagg gattcctcca taacgaaaat gactattcat accgattctg cagccattgt
     3361 tgtaaac
//
LOCUS       DAORTI010000242         3274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_40124_length_3274_cov_5.740603, whole genome shotgun sequence.
ACCESSION   DAORTI010000242 DAORTI010000000
VERSION     DAORTI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40124_length_3274_cov_5.740603"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..3238)
                     /locus_tag="PLO70_16690"
     CDS             complement(26..3238)
                     /locus_tag="PLO70_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ76159.1"
                     /translation="MIRMLKKVGLCLLLLTALSPLNSIFAQGRQITGTVTSSENKQPV
                     ASATVTVKGTKNATTTDAQGNYKITVDSKATTLVFTSASFIAFETSINGRTVIDAELI
                     AEVRALEDVVVVGYSTIKRKDLTGSVSSVNSKQLKDFPLSSAAEALQGKLAGVQLTSS
                     EGAPGAEIIVRVRGGGSITQDNSPLYIVDGVQVENALSVLSPQDIATVDVLKDASTTA
                     IYGARAANGVVIITTKTGKAGKTQVSYNGSIGYRQLAEFQEVMKPYDFVVWQYERTRA
                     TATDSASFAQTYGTTWDTLANYKNTNFINWQDRVFGRKANFATHNVSVNGGNQTTTFN
                     LSLTANKEDGIQIESGFNRKLVNFKLDHKASDKLRVGFTARYLDQAIQGAGTTNSGTR
                     TTNRLRHAINYRPFELDRPGFGIDDFDEAYYLASAGAINPVILTQAEYRKQYTKAVYL
                     TAYFNYNILKNLSFRSTLGFDNASVRTDLFYSKITGTARNFGSLPVASIGQQNNYTIS
                     NSNILQYTLNNYKQHHDLTLLAGQEVVDVRSKQNTFETRYFPAEISPEKALANMGLGS
                     APTGSAQPLPTSFEEPAARIASFFGRVSYGYDDKYLVNFNLRADRSSKFSSDNGTLVF
                     PSGSVAWRFSREKFFEKATWLNDGKLRFGYGSVGNNRIGNLLYQQLYGVTGQYAFNHS
                     ILPGFAPTALANPSLRWEKNTTSNFGIDLAMFKNRVQLTVDVYKNQAKDLLLAVAIPP
                     TTGYTSQLQNIGATSNRGVEFQLNATPVQKKDFSWNSNFNIAFNKNKVESLGGVQSQT
                     RNSGWQGSDGVDDYLVQVGQPIGLMYGFVTDGFYKVDEFDYNTTTGVYTIKPGIAVNG
                     VYGIPQPGMLKWKDLNGDGVITADADRQVIGNANPDFTGGWSNQFTYKNFDLSVFVNF
                     VVGNDIYNANKLEWTDGAFPNLNTLDIMKNRFTYINASGIRITDPTELTKMNANAEIW
                     TPVRVQRWWLHSWAIEDGSYLRFNNVTLGYSLPKSMLSKLKISNFRIYGTVNNLGTIT
                     NYSGYDPDVTARRSDPLTPGVDFAAYPRARTWLFGVNVTF"
BASE COUNT          862 a          719 c          711 g          982 t
ORIGIN      
        1 tttcatatcg aatagttttt gtttgttaga aagtaacgtt tacaccaaat aaccatgttc
       61 ttgccctggg ataggctgca aagtccacac ccggtgtcaa tggatcactt ctccgggctg
      121 ttacatcagg gtcataccct gaataattgg tgatagtgcc caggttgttc accgtaccat
      181 atatcctgaa gttggagatc ttcaatttgc ttaacatact ttttggaagt gaatagccca
      241 gtgtaacgtt attgaaacgg agatatgatc cgtcttcaat ggcccaggaa tgtaaccacc
      301 agcgttgtac tcttaccggc gtccaaatct cagcattggc attcatcttg gttaattctg
      361 taggatcagt tatccggata ccggaggcgt taatataggt gaacctgttt ttcatgatat
      421 ccaatgtatt caggttcggg aatgcgccat cggtccattc aagtttgttc gcattataaa
      481 tatcgttacc aaccacaaag tttacaaaaa cggacaggtc gaaattctta taagtaaact
      541 ggtttgacca gccacctgtg aaatcaggat tggcattacc gatcacctgc ctgtctgcat
      601 cagcggtgat aacgccatca ccgttcaggt ctttccattt aagcatgccg ggctggggaa
      661 taccatatac accgtttacg gcaattccgg gtttgatggt ataaacaccc gtggtagtat
      721 tataatcaaa ttcatccacc ttatagaaac catcggttac aaaaccatac attaaaccaa
      781 ttggctggcc tacctgcacc aggtaatcat ctacaccatc agatccctgc cagccggagt
      841 ttcttgtctg cgattgtaca ccacccaggc tttcaacttt gtttttgttg aaagcaatat
      901 tgaaattaga attccagcta aagtcttttt tctgaaccgg ggtggcattc aattgaaatt
      961 ccacgcccct gttagaagtg gcgccgatat tctgcagctg tgaagtgtaa cctgttgttg
     1021 gaggaatggc tacagctaac aacagatctt ttgcctggtt cttataaaca tctaccgtta
     1081 attgcaccct gtttttgaac atggccaggt ctattccaaa gttgctggtg gtattttttt
     1141 cccatctcag actgggattg gccaacgcag ttggagcaaa gccaggtaaa atagaatggt
     1201 tgaatgcata ttgtccggtt acaccataca actgctgata cagcaggttg ccgatacggt
     1261 tattacctac agaaccatat ccaaaccgga gctttccatc attcagccag gttgcttttt
     1321 cgaagaattt ttccctggaa aatctccagg ccacagaacc ggaaggaaat accagtgttc
     1381 cgttatcaga actgaattta gaagaacggt cagccctgag gttgaagttt accaaatact
     1441 tatcatcata accatagctc acccggccaa agaaagaagc aatgcgggca gcgggttctt
     1501 caaaggaagt aggtaagggc tgagccgaac cggttggggc agaacccagt cccatattgg
     1561 ccagtgcttt ttctggcgaa atctcagccg ggaaataccg ggtttcgaat gtattttgtt
     1621 ttgaacgaac atccacaact tcctgccctg ccaggagggt caggtcatga tgttgtttat
     1681 aattatttaa ggtgtattga aggatatttg aattactgat cgtgtaatta ttctgctggc
     1741 ctatagaagc caccggcaga gagccaaagt ttcttgcagt accggtgatc ttactgtaaa
     1801 acaggtccgt tcttacagaa gcattgtcaa atcctaaggt agacctgaaa gaaagatttt
     1861 taaggatatt atagttgaaa taagcagtga ggtatacagc tttggtatat tgtttcctgt
     1921 attctgcctg ggttaagata accgggttta ttgcaccggc agaggccagg tagtacgctt
     1981 catcaaaatc atcgattccg aaaccgggtc tgtccagttc aaatggcctg tagttgattg
     2041 catgacgaag acggttggtg gtcctggtgc cgctgtttgt agttccagca ccttgtattg
     2101 cctggtcaag ataacgggca gtgaaaccta ctcttagttt atcagatgct ttatggtcca
     2161 gtttaaaatt caccagtttc ctgttgaacc cggactcaat ctgaatacca tcttctttat
     2221 tagcggtgag acttaaatta aatgtagttg tttgattgcc tccgttcaca gagacattat
     2281 gtgttgcaaa atttgctttt cttccaaaga cacggtcctg ccagttaata aaattggtgt
     2341 tcttatagtt ggctaatgta tcccatgtgg tcccgtaggt ttgggcaaaa cttgcactat
     2401 ctgtggcagt ggctcttgtt ctttcatatt gccaaaccac aaaatcataa ggtttcatta
     2461 cctcctggaa ctctgccagc tggcgatagc ctatcgaacc gttgtagcta acctgggttt
     2521 ttccggcttt accggttttg gtggtaatga tcactacacc atttgcagcc ctggcgccgt
     2581 agatagctgt tgtagatgca tctttcagta cgtccacggt agcaatatcc tgcggagaaa
     2641 gaaccgataa tgcattttca acttgtactc catcaacaat gtataagggt gaattgtctt
     2701 gtgtgatcga accaccgcct cttaccctga caatgatttc agccccggga gcgccttcac
     2761 tcgatgttaa ctgcacaccg gccagttttc cctgtaaagc ttctgctgca gacgaaaggg
     2821 gaaaatcttt taattgtttg gaattcactg aagaaacaga acctgtcagg tcctttcttt
     2881 taatagtact gtaacctaca actaccacat cttccagtgc tctgacttct gcaatcaatt
     2941 cggcatcaat tacagtcctg ccattaatgc ttgtttcaaa agctatgaaa gaagcactgg
     3001 taaacacaag ggtggtggct ttgctgtcca ctgtgatctt gtaattaccc tgtgcatcag
     3061 tggtggtagc atttttagtg ccttttaccg ttactgttgc actggctacc ggctgtttgt
     3121 tttcggagga ggtaacagta cctgttatct gccttccctg tgcaaaaatt gaattgagcg
     3181 gagatagggc tgtcagcagc agcaaacata atcccacttt ttttagcatt ctgatcatag
     3241 tgcagttttt gatgataacc tgaaatgaag ttta
//
LOCUS       DAORTI010000243         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_40206_length_3270_cov_4.768896, whole genome shotgun sequence.
ACCESSION   DAORTI010000243 DAORTI010000000
VERSION     DAORTI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40206_length_3270_cov_4.768896"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLO70_16695"
     CDS             complement(<1..313)
                     /locus_tag="PLO70_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76160.1"
                     /translation="MLDKVKSRKAGNKIIVFVLLFSWINASAQKETPEDTIAKPLITA
                     VGKPDGKKTEIKIKKDEARLRSSDGMLELIIPVGAVSPKTIISIQPITNTMSNGNGPA
                     YR"
     gene            complement(333..2144)
                     /locus_tag="PLO70_16700"
     CDS             complement(333..2144)
                     /locus_tag="PLO70_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76161.1"
                     /translation="MKKWNNSYKNNSFVTSYSFTWHRSLRRTLVLSVFMASLSIQTFS
                     QTETFDIATYTPPKDFKKDSKAAVVNYTNVNQATGSICVIALFASTVSTGDAKKDFKK
                     AWQELAVTPYKAEANPKTEMQTTTDGWEVVTAAAPIKLDGAELYVILTVASGFGKTMS
                     IRTSLNDEAYTAQIDAMFASMELDKTKTSSMSNNNSTTTSITGTIGKFGLMNYTSPAG
                     WSHQSFPDGVVFKPLDIPPGEYLSIQIMQPLNFSGSMEQALQQSFDEAAAMYNGTKMN
                     YAGSGASYQKTEAKKSFQGWEYIRANGGIRIGTGDYPPEYGLDMFVTKINNRFERMAI
                     LKSRTINRSCSMSAFYADERQQYKTAIDNFLFSLQFTDGPQPLITQSKSLEGGGIKAV
                     WRGISMNVSTATTSQPIGLGYNVFTPIFLSNGQAYFGPKFYSEGLNDVDTRVLAELYR
                     RDWGTYTFSTGRGVLKMPYGDIPLRMEGNTLIITANHTDHRFYQLPSVDGAKFNGTYT
                     MSEAYGKIPAITFSADGRFSDNGAIRVLYHEYNECINPALAPGSGAYEVKDYTITFSY
                     SDGRKIKIAFLGTEYDINNQSPSMLRMSNNEDPMTRQ"
     gene            complement(2128..>3270)
                     /locus_tag="PLO70_16705"
     CDS             complement(2128..>3270)
                     /locus_tag="PLO70_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76162.1"
                     /translation="YNMNHLLYQFKKRRRPLVFVCLLMAGFGSNCMTQTPKLTNAFAA
                     SSGIAVDSKGNAFVTGQNNKVIKITAAGKAELFAGGGRNRKDGLAKEIRFSSLAGIAI
                     DSSDNLYVADGTRIRKITPEGFVTTIAGSEISGYKDGDRSIATFVNLENIAIDNKGTI
                     YVTDNEFTNNRGSSGHHVIRKISAEGIVTTIKNGNEAELRVHYARGIACDKDGNLYVN
                     ASVSHCIKKISPSGVITTVAGQCDKTKFNSVYKEGPVSTAVLTNPSGMAIAKNGDIYI
                     TDTRLHRIIKIANNKVTTVAGSGKFNFSGNPAGAAEPGFADGKTTAARFNAPAGIAFD
                     RNGNLFIVDASSSNNSYIRKLSPEGMVTTFCKHEWNPKTSQYEEVE"
BASE COUNT          821 a          701 c          664 g         1084 t
ORIGIN      
        1 aacgataagc cgggccattt ccattggaca ttgtatttgt aatgggctga atgctgataa
       61 tagtttttgg agaaacggcc ccgacaggta ttatcaattc cagcattcca tcagaagacc
      121 gtaacctggc ttcatctttt tttattttga tttctgtttt ctttccgtcg ggtttgccca
      181 ctgcggtaat aagaggtttt gcaatagtat cttccggtgt ttctttttgt gcggatgcat
      241 tgatccagct aaacagcaaa acaaaaacaa ttattttatt tcccgctttg cgggatttca
      301 ctttgtccaa cataatttat tttttataat gattattgtc ttgtcatcgg gtcttcatta
      361 ttactcatcc gcaacatgga cgggctttga ttgtttatat catattccgt tcccaggaat
      421 gcaattttta tttttcttcc gtcactgtaa ctgaatgtga tcgtataatc ttttacttca
      481 taagcgccgg atccgggtgc aagggccggg ttgatgcatt cattgtattc atggtacaaa
      541 acccttatag cgccattatc actaaacctt ccatcggcag agaaagtgat ggctggaatt
      601 tttccatatg cttcactcat cgtataagtg ccgttgaatt ttgcaccgtc aaccgaaggc
      661 aattgataaa aacggtgatc ggtatgattg gcagtgatga tgagtgtgtt tccttccatt
      721 cttagtggga tatcgccata aggcattttc aaaactcctc ttccagtgct gaaggtataa
      781 gtgccccaat cccggcggta taattctgca agtacccttg tgtcaacatc attcaaacct
      841 tcggagtaaa atttagggcc gaaataagct tgtccgtttg ataaaaatat tggcgtgaaa
      901 acattatagc ctaaaccaat cggctgactg gtagttgctg tgctgacatt catcgaaata
      961 ccacgccaca cagctttgat gccaccacct tcaaggcttt tagattgggt gatgagtggt
     1021 tgtggtccgt cagtaaattg cagagagaat aaaaaattat caatcgcagt tttgtattgc
     1081 tgtctttcat cagcataaaa tgcagacatg ctgcaactcc tgttgatcgt tcttgatttt
     1141 aaaattgcca tcctttcaaa ccggttattg atttttgtaa cgaacatatc caatccatat
     1201 tcggggggat aatcaccggt tcctatccgg atgccgccat ttgctcttat atattcccat
     1261 ccctggaagg atttttttgc ttctgttttt tgatagctgg ccccgctacc ggcatagttc
     1321 atctttgttc cattatacat ggcagccgct tcatcaaaac tttgctgcag tgcctgttcc
     1381 atactgcctg aaaaattcaa cggctgcatt atttgaatgg ataaatattc acccggtggt
     1441 atatccaatg gtttaaacac aactccatcg gggaatgact ggtggctcca acccgccgga
     1501 gatgtataat tcatcaagcc aaattttccg atagttccgg ttatagatgt agttgtactg
     1561 ttattattac tcatggatga tgtttttgtt ttatctaatt ccattgaggc aaacattgca
     1621 tctatttgtg cagtgtaggc ttcatcgttt aatgatgttc tgatgctcat cgtttttcca
     1681 aagccgcttg ctacggtcag gataacatat agctctgccc catctaattt tattggcgct
     1741 gcggccgtca ccacttccca tccatcagtt gtggtttgca tttctgtttt tggattggct
     1801 tccgccttgt acggagttac tgctaactcc tgccatgctt ttttaaaatc ttttttcgca
     1861 tcgcctgtgc tgacagtact ggcaaacagt gcaattacac aaatgctgcc ggtggcctgg
     1921 tttacgttag tataatttac cacagcagcc ttgctgtctt ttttaaaatc ttttggcggg
     1981 gtataggtgg caatatcaaa tgtttctgtt tgagaaaatg tctggatgga taatgatgcc
     2041 atgaaaacag aaagtacaag agtgcgacgc aacgaacgat gccatgtaaa actgtagctg
     2101 gttacaaaag aattattctt gtatgaatta ttccacttct tcatattgac ttgttttagg
     2161 gttccattca tgtttgcaaa atgtagttac cattccttcc ggtgatagct tccggatata
     2221 gctgttattg gaactgctgg catccacaat aaataaatta ccattccggt caaaggcgat
     2281 acctgctggt gcattaaaac gtgcggcagt tgttttgcca tcggcaaagc cgggctctgc
     2341 agcgccggcg gggttgccgg agaaattaaa tttaccggaa cccgccaccg tggttacttt
     2401 attgttggcg atttttatga tgcggtgcag tcttgtatca gtgatataga tgtcgccgtt
     2461 ttttgcaata gccatcccac ttgggttggt caatacagcc gttgatacag ggccttcttt
     2521 atacacggaa ttgaattttg ttttatcaca ctggccggca acagtggtaa tgacacctga
     2581 aggagaaatt tttttaatac aatggcttac gctggcgttt acatataaat taccatcttt
     2641 atcgcaggca atgcctcttg cataatgcac ccggagttca gcttcattcc cattttttat
     2701 tgttgtaaca atgccttctg ccgaaatttt ccggataaca tgatgcccgg aacttcccct
     2761 gttattggtg aattcattat ccgtaacata aatagttcct ttattatcta tggcgatatt
     2821 ttcaagattg acaaaagtcg caattgagcg gtcgccgtct ttatacccgg atatttcact
     2881 accggcaatg gtggttacaa aaccttccgg tgtaattttt ctaatccttg taccatctgc
     2941 tacatacaaa ttatcggagg agtcaatggc gattcctgca aggctggaaa accttatctc
     3001 ttttgccagg ccatctttac ggtttctccc accacctgca aagagctccg cttttccggc
     3061 tgctgttatt tttattacct tattattctg gcccgttaca aaagcattgc ccttactgtc
     3121 cactgcaatg cctgatgatg cagcgaatgc attggtgagt ttgggtgttt gcgtcataca
     3181 attacttcca aaaccagcca tcaaaaggca aacaaataca agaggcctgc gtcttttttt
     3241 gaactgataa agtaagtgat tcatgttgta
//
LOCUS       DAORTI010000244         3262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_40354_length_3262_cov_4.516994, whole genome shotgun sequence.
ACCESSION   DAORTI010000244 DAORTI010000000
VERSION     DAORTI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40354_length_3262_cov_4.516994"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..2040
                     /locus_tag="PLO70_16710"
     CDS             97..2040
                     /locus_tag="PLO70_16710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ76163.1"
                     /translation="MRKYSLVLLLIIGILPANFSAAQLCSGSLGDPIVNNTFGNGTNP
                     GPQLSTAATSYQYVSTDCPTDGFYTVRNNTFNCFSDTWHSLSIDHTGDANGYFMLVNA
                     SVQPGAFYIDTVRGLCAGTTFEFAAWVINMLKQTACSGSGITPNLTFLIERTDGMVIQ
                     SYSTGDITSLSSPLWKQYGFFFATPPGITDVVLRIINNAPGGCGNDLALDDITFRPCG
                     PLLSPTIIGSSSGSVTICEGANTVINFASSISSGYSNPEFQWQESINGGVFVDIPGAT
                     AINYTRNIVAAMPGNYQFRLTVAEAGNLTNIGCRVVSSTLRLQVATLPVTSISIAGSL
                     CEKSDLLLTATGGTQYDWNGPNSFVATGNPVLISNAQPLNSGKYFVIVENAAGCSNID
                     SVVVIVNPSPVASVLFTSNTICEGEMVQLSATGGGTYTWSPGTGLSTSNDPAPIASPS
                     STINYSVIVTNSFNCTDTAFSEVKVLKKPFANAGPDRGTSEGSPVQLLGSISGDDIRY
                     FWSPPLYLNNTTDVQPLVTAPGGTYNYTLTVISNVGCGTVFDDVNITVYNGIYIPTAF
                     TPNNDGRNDRWQIPALSIFSEFQLSVFNRYGDIVFSTKNQQASWDGTHKGEQQPVGVY
                     AYILSLNDNGEKLFYKGIITLIR"
     gene            2106..3068
                     /locus_tag="PLO70_16715"
     CDS             2106..3068
                     /locus_tag="PLO70_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76164.1"
                     /translation="MALLEVVGAGKKEKENFTVKDINFFQQPLEKIAIAGETGSGKTT
                     LLKMIGGLAQLDKGNIYFEGERVLGPLERLLPGHPQIAYLSQHFELRNNYRVEEELES
                     KNLLTEEAANNIYHICRIEHLLKRKTDQLSGGERQRIVLARLLTTCPKLLLLDEPFSN
                     LDAIHKNIIKSVLLDIATQLNTSCILVSHDAADTLSWADTIYVLKEGMIVQQGTPQKI
                     YNEPIDEYCAGLFGEYNLLNAANAKQFFPNAADKEQLIVRPEQFSITTESSNAAKGIV
                     QAVYFFGSYYVVDVLVNGQLLRIRTGEKDLAIEDEFFLAMTNLL"
     gene            3068..>3262
                     /locus_tag="PLO70_16720"
     CDS             3068..>3262
                     /locus_tag="PLO70_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76165.1"
                     /translation="MFWRLAKVAIFTTNVDAESNWLINDKTVCGALNRHFCQTAVIGC
                     FAYLSVSFLCRLVQRVRCVKV"
BASE COUNT          962 a          570 c          681 g         1049 t
ORIGIN      
        1 ataaaagcca aagtagtttg gtttgtttca aaaaaaatgt agtatggttt aaaccaatga
       61 tataatgtat tttagcaatc tatcaaaccg gatgtgttgc gaaagtattc actagtatta
      121 ttgttgatca ttggaatatt gcctgcaaat ttttccgctg ctcagttatg ttctggaagt
      181 cttggtgatc ccatcgtcaa taacacattt gggaatggaa caaatccggg acctcagcta
      241 tcaacagctg ccacgtctta ccaatatgtt agtaccgact gcccaacaga tggtttctat
      301 acagttagga ataatacttt caattgtttt tcagatacat ggcatagtct ttccattgat
      361 catactggtg acgccaatgg ttattttatg ttggtaaatg cttctgtaca gccgggggct
      421 ttctatatcg atacagtaag aggattgtgt gcaggtacta cttttgaatt tgcagcatgg
      481 gtaatcaata tgctaaagca aactgcttgt agcgggagcg gcatcactcc aaaccttaca
      541 tttttaattg aaagaactga tggcatggta attcaatcgt atagcactgg tgatattact
      601 tcgctgtcat cacctctctg gaagcaatat ggatttttct ttgcaacacc tcctggtatt
      661 acagatgtag tacttcgtat tattaacaat gctcccggtg gatgtggaaa tgatctcgca
      721 ctggatgata taactttcag gccatgcgga cctttgctta gcccaactat tattgggtca
      781 tcatctggat cagttacaat ttgcgaagga gcaaatacag taattaattt tgccagttct
      841 atttcttctg gatattctaa tcctgaattt caatggcagg agagtatcaa tggtggggta
      901 tttgttgata tacctggtgc taccgcaata aattatacaa gaaatatagt tgcagctatg
      961 cccggaaatt accagtttcg tttgactgtt gctgaggctg gcaatcttac taatattggg
     1021 tgtagagtag tatcctcaac tcttaggctg caggtagcaa cattgcctgt tacgagtatt
     1081 tctattgccg gatctctttg tgaaaaaagt gatctgctat taactgctac tggaggaact
     1141 caatatgact ggaacggtcc aaacagtttt gttgcaactg gtaatcctgt tttaatttca
     1201 aacgctcagc ctcttaattc aggaaaatat ttcgtaattg tcgaaaatgc tgcagggtgc
     1261 agtaatatag attcagttgt tgttattgtt aacccttcac ccgttgcttc agttttattt
     1321 acttcaaata caatttgtga aggtgaaatg gttcagttat ctgcaacagg tggtggtact
     1381 tatacctggt cgccaggtac cggtctgtca acaagtaatg acccggctcc aattgcttct
     1441 ccatcatcaa ccataaatta cagcgttatt gtaacgaatt cttttaattg tacagatact
     1501 gctttttcag aagtgaaagt tttgaaaaag ccttttgcaa atgctggccc cgatagagga
     1561 actagtgaag gttccccagt gcaattattg ggaagtatat caggagatga tattcgttat
     1621 ttttggtcac cgcctttgta tttaaataat actacagatg ttcaaccttt agtcactgct
     1681 ccgggtggta cctataatta tacgcttaca gtaatatcca atgttggttg tgggacagtt
     1741 tttgacgatg taaatattac agtgtacaat ggtatatata taccaacagc ctttacacca
     1801 aataatgatg gaagaaatga ccgttggcag atacctgcat tgtcaatatt ttctgaattt
     1861 caactctctg ttttcaatag gtatggtgat atagtttttt ctacaaagaa tcaacaggca
     1921 tcctgggatg gaacacataa aggtgagcag caaccagtag gagtttatgc gtatatactt
     1981 agcctgaatg ataatggcga aaagttattt tataaaggta ttattacatt aatcagataa
     2041 ttttttatta gcgttgatat gcatcttaat ccttataaat taatttacaa catttatttt
     2101 cgggtatggc tttattagaa gtagttggcg caggcaaaaa agaaaaggaa aactttacag
     2161 ttaaggatat taatttcttt caacaaccgt tggagaaaat tgctattgca ggagaaacag
     2221 gttctggcaa aacaacttta ttgaaaatga ttggtggcct tgctcaactc gataaaggca
     2281 acatttattt tgaaggtgaa agagttcttg gtccgttgga gagattactt cccgggcatc
     2341 cgcagattgc atacctgagc cagcattttg aattaagaaa taactataga gtagaagaag
     2401 aactggaaag taaaaacctg ttgacggaag aagccgccaa caacatttat cacatctgcc
     2461 ggattgaaca tttattaaaa agaaaaacag accagctatc cggcggcgaa aggcaacgca
     2521 ttgtattggc aagattactt actacttgtc ccaaattatt attgctggat gaacctttct
     2581 ccaatcttga tgcgatacac aagaacatta ttaaatctgt gctgctggat attgccactc
     2641 aattaaacac cagttgcatt ttggtatcgc atgatgcggc ggatacactc tcctgggctg
     2701 atacaattta tgttctgaaa gaaggaatga tagtacagca aggcactcct caaaaaatat
     2761 ataacgaacc tattgatgaa tattgtgcag gcctgtttgg tgaatacaat ttgcttaatg
     2821 cagcaaatgc aaaacagttt ttcccaaatg ctgctgataa agaacaatta atcgttcggc
     2881 cggagcaatt tagtattact actgaaagca gtaatgctgc aaaagggatt gtgcaagctg
     2941 tttacttttt cggtagttat tatgttgttg atgtgctggt gaatgggcag ttgttgcgga
     3001 ttagaaccgg ggagaaggat ttggctattg aggacgagtt ttttttagca atgacaaatc
     3061 ttttataatg ttttggcgct tggcgaaggt ggcaattttt accacaaatg ttgatgcgga
     3121 gagcaattgg ttgattaacg acaaaactgt ctgcggagca ctgaaccgcc acttttgcca
     3181 aaccgctgtt atcggttgtt ttgcttatct gtccgttagt tttctttgtc gtcttgttca
     3241 acgtgtaaga tgtgtaaaag tc
//
LOCUS       DAORTI010000245         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_40676_length_3246_cov_6.379505, whole genome shotgun sequence.
ACCESSION   DAORTI010000245 DAORTI010000000
VERSION     DAORTI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40676_length_3246_cov_6.379505"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="atpF"
                     /locus_tag="PLO70_16725"
     CDS             complement(<1..484)
                     /gene="atpF"
                     /locus_tag="PLO70_16725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQZ76166.1"
                     /translation="MKLLTPEFGLLIWTLLAFLIVFFILKKLAWPAIIKGLRDRENSI
                     TDSLATAQKVKEEMAMMKSENEALLTKAREERALMLKEARETKDRIISEAKEQAKAEA
                     SKIVTEAQAAINAQKMAAITEVKNQVGKLVIEVSEKVLRKELGNKEAQEAHIKGLVDE
                     V"
     gene            complement(571..816)
                     /gene="atpE"
                     /locus_tag="PLO70_16730"
     CDS             complement(571..816)
                     /gene="atpE"
                     /locus_tag="PLO70_16730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQZ76167.1"
                     /translation="MDLLNVILLEATANAGGAIGAGLAAIGAGIGIGQIGKGAVEAIA
                     RQPEASNDIRGNMILTAALVEGAALFAIIVGFLAMVL"
     gene            complement(849..1991)
                     /gene="atpB"
                     /locus_tag="PLO70_16735"
     CDS             complement(849..1991)
                     /gene="atpB"
                     /locus_tag="PLO70_16735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQZ76168.1"
                     /translation="MLVRRFKSFTVAVFSLFFVLFSLQAFAGGGEGEKDGEKKKFNAS
                     EVIFGHVLNGHEFHFMDIGGKPVTIPLPVILYSPQKGFTSFMSSKFEHGHHNHNGYAM
                     LTDHSIKEMGLDPKKFAVGDIVAVDENGKYDAAVKIYDLSMTRNVVQMILALIVFVWI
                     MLRIAKRYKSGVGVTSAPKGSQSLLEPVITFVRDEVAKPNLGHKYEKYLPYLLTVFFF
                     ILINNIFGLIPGSANVTGNIAFTAVLGLISFVVILFSSNKHYWGHIFNPPGVPLGVKF
                     ILVPVEFLSVFIKPFALIIRLFANMVAGHIIIICLISLIFIFGELNPIAGWGASPLSI
                     GFTVFIYLIEVLVAFLQAFIFTMLTAVFIGQALEGEHHDEAHAVHH"
     gene            complement(2153..>3246)
                     /locus_tag="PLO70_16740"
     CDS             complement(2153..>3246)
                     /locus_tag="PLO70_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76169.1"
                     /translation="EKFKKINREHRNANIKAIFAYSVFFPLVEIVLALSIGLVVWWTS
                     KEALDLHQSQQGTVISFILCFNLLFRPLRMIADKFNVLQMGIIASERVFKVLDNDDFT
                     PADPSDAYTPEVVKGKIEFEKVVFEYNPGVPVLKDVSFLVNPGETIAIVGHTGSGKST
                     IISLLNRLYHIQKGSIKIDDVNIEQFNLEILRKNIGVVLQDVFLFSGSVIDNITLRNP
                     EISREKVTEAAKLIEMHDFIMRLPGGYDYNVMERGATLSLGQRQLLSFIRAMLYNPSI
                     LILDEATSSVDTESEQLIQHAIDTLIKGRTSIVIAHRLSTIRKAHKIIVLDKGEVKES
                     GTHEELIKKGGFYAQLHEMQFQETNPENS"
BASE COUNT          968 a          751 c          600 g          927 t
ORIGIN      
        1 tgacttcatc aaccagccct ttgatatgtg cttcctgggc ctctttattt cctaactctt
       61 ttctgaggac tttctcactc acttcaatta ccagtttacc aacctggttc ttcacctctg
      121 ttatagcagc catcttctgt gcattgatag cagcctgagc ttccgtaaca attttacttg
      181 cttccgcctt agcctgctct tttgcttcgc tgatgatcct gtcctttgtt tccctggctt
      241 ctttcagcat caatgctctt tcttcacggg ccttcgtcag caacgcttca ttctcacttt
      301 tcatcatcgc catctcctct ttaactttct gtgcggtggc caatgaatcg gtaatactgt
      361 tttcacggtc tctcaaccct ttaataatgg caggccatgc cagtttcttt aaaataaaga
      421 atacgatcaa aaaggccagc aaggtccaga tgagtaaacc aaattcggga gtaagaagtt
      481 tcattgtcta tttcagtttc ttgtttcaga tttaaatact gcatcctccg ccctgtgcat
      541 tggatgcagt aaatttttgt ggcaacgata ttataatacc attgcaagga agcctacgat
      601 gatcgcaaac aacgcagcac cttcaaccag ggctgcagtc aggatcatgt ttccgcggat
      661 gtcgttagac gcttccggct gacgggcaat cgcttcaaca gcgcctttac cgatctgacc
      721 gataccgata ccggcaccga tagcagcaag acctgctcca attgcaccac ctgcgttagc
      781 agtggcttcc aacagaatta cattcagtaa atccatgttt attattattg atagttaaaa
      841 aatacttttt aatgatgaac cgcatgcgcc tcatcgtgat gctcaccttc cagggcctgt
      901 ccgataaata ctgctgtcag catcgtaaag atgaaggctt gtaagaaggc caccagtact
      961 tcgatcaggt atataaatac agtaaaccct attgacagcg gagatgcacc ccatccggca
     1021 attggattta actctccgaa aatgaatatc aatgaaatca aacaaataat gataatgtgc
     1081 ccggccacca tattggcaaa aagacgaatg ataagggcaa agggcttaat gaacacactc
     1141 agaaactcta ccggtaccaa aatgaactta acacccagtg gtacccccgg agggttgaag
     1201 atatgtcccc agtagtgctt attggaactg aacaatatca ctacaaagga tatgagacca
     1261 agtactgctg taaaagcaat attacctgtt acgttagcag aaccgggaat caaaccaaaa
     1321 atattattga taaggatgaa aaagaaaaca gtaagcaggt aaggaagata cttctcatac
     1381 ttatggccaa gattcggttt tgctacttca tcccgtacaa atgtgataac aggctccagc
     1441 aggctttgag aaccttttgg ggcagaggta acacctactc cgcttttata tctttttgcg
     1501 atcctcagca ttatccaaac gaaaactatc agcgccaaaa tcatttgcac cacgttgcgg
     1561 gtcattgaga ggtcgtaaat ttttacagca gcatcatact tgccattctc atcaaccgct
     1621 acaatatcgc caacggcaaa tttcttggga tctaatccca tttctttaat tgaatgatca
     1681 gtgagcatgg cataaccatt atggttatga tgtccgtgct caaacttaga agacatgaag
     1741 ctggtaaacc ctttctgtgg agaataaagg atgacaggaa gtgggatggt aaccggtttg
     1801 ccgccaatat ccatgaaatg gaactcatgc ccgttcaaaa cgtggccaaa tatgacttcg
     1861 gaagcattaa atttcttctt ttcaccatct ttttcgcctt ccccaccacc agcaaaagcc
     1921 tgcaatgaaa atagaacaaa aaataagctg aaaaccgcta cagtaaagga tttgaaacga
     1981 cgtactaaca tacctgttcc gaaatttggc gcaaagataa gggggtgggg gcaaaaacga
     2041 accgggattg aaaggaattt agtgatttat acaaaaatga atatcagtaa tcattgtaca
     2101 ccattcttca ccagcagtaa attctgatta tcaaggtaat gaacctctga aatcagctat
     2161 tttcagggtt agtttcctgg aactgcatct catgtaattg ggcataaaaa ccaccctttt
     2221 ttattaattc ttcatgagtg ccggattcct ttacttcacc cttgtcaagt acaataattt
     2281 tatgcgcttt tctgatcgtc gaaagccggt gagcaataac gatagatgtc cggcccttta
     2341 tcagggtatc tattgcatgc tgaatcagct gttcactttc tgtgtctacc gaagaagtag
     2401 cttcatccag gatgagaatg gatggattat acaacattgc ccgtataaat gacaataatt
     2461 gacgctgccc cagcgataaa gtagctcctc gttccatcac attgtaatca tatccgcctg
     2521 gtagccgcat aataaaatca tgcatctcaa tgagtttggc cgcttccgtt actttctctc
     2581 ttgaaatttc agggttccgc agtgtgatat tgtcaatcac cgaacctgag aacaaaaaaa
     2641 catcctgtag tactactcca atatttttac gcagaatttc aagattgaac tgttcaatat
     2701 ttacatcatc tattttaata cttccttttt gaatatgata caaccggttc agcaaactga
     2761 taatcgtcga tttgccgcta ccggtgtggc caacgattgc aatggtttct cccgggttaa
     2821 ccaaaaagga aacatcttta agcaccggaa ctccgggatt gtattcgaat actacctttt
     2881 caaattcaat tttacctttt actacttcag gtgtataggc atccgaagga tcagcaggag
     2941 taaaatcatc attatccaat actttaaaca ccctttcgct ggcaatgata cccatctgca
     3001 atacattgaa tttatcagca atcatcctga gcggacgaaa cagtaaatta aaacaaagaa
     3061 taaaagatat aaccgttcct tgctggcttt ggtgcaggtc cagcgcctct ttggatgtcc
     3121 accatacaac caaaccaata gataaggcca gtacaatttc cactaacggg aaaaaaaccg
     3181 agtacgcaaa aatggctttg atattcgcat tacgatgctc cctgttaatc ttcttaaact
     3241 tttcaa
//
LOCUS       DAORTI010000246         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_41890_length_3185_cov_3.999681, whole genome shotgun sequence.
ACCESSION   DAORTI010000246 DAORTI010000000
VERSION     DAORTI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41890_length_3185_cov_3.999681"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLO70_16745"
     CDS             <1..1114
                     /locus_tag="PLO70_16745"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ76170.1"
                     /translation="AGLLFACSGIVLAQPVEVDLFQKVATATTSQIGVRVRATGANID
                     YIGVTFYILYQSANAVPQSAGVNTVVGVDDSRMVTTFAWGSSARFTNPAQVVSIDPGV
                     PGGQTYDRRYVYGNIDETGGSNIRTLTTAWDTLLYITLNTLQPTFPQGGHGYIQSTAE
                     AAGAALSDPGFSNVAFLVNSGDRPLGLSTLPVTFTKYDVTCNDKGALLTWSTGSEQNS
                     SKFEIQRSLNGIDWISIDNVAAAGNSTDQRNYQYLDLYGGTAFYRIRQVDIDGRFVYT
                     AVKRTECQKSQYDVALYPVPARDNLNVVIRSEINTRTEIQILDINGRSLRRVAVQINK
                     GNNNIQLSVGNLPAGQYMLVGSDPSIQINKKFTIIR"
     gene            1526..>3185
                     /locus_tag="PLO70_16750"
     CDS             1526..>3185
                     /locus_tag="PLO70_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQZ76171.1"
                     /translation="MRKIFLLLNFCFIAFFSIAQIGINTGTPWEFLKGDSLVPQSAPV
                     YGNLGTEAEGNRPGRRAKASTWIDGSGYLWLYGGSDATFGGSSDLWKYDPTKNQWIFI
                     KGNNSRNVAGVYGIKGVSDIRNNPGAGSYSATWIDNTGNFWLFGGVNKNHLWRYNPVI
                     NEWTWMKGDNSGVAPGVYGIQGIAANQNNPGVRSGCVSWKDATGNLWLFGGSGLGASG
                     SSGNLNDLWKYNPITNHWTWMKGDSTVGSLGVYGSLGVPHPDNKPPARNRSNSWVDAS
                     GNFWLFGGWAFEPPGTTKYFNDLWKFDPVTNQWTWMKGDSVINQLGIYGSIGVPASTN
                     KPGSRNDATSWTDGAGNFWLFGGFGYSASSNGRLNDLWKYNPVNNEWVWVKGDNTINN
                     SVVYGTQGVFAVANKPGGRGAASSWADNSGNLWLFGGIEHRGNLVELSDLWKYDTLLN
                     QWAWIKGPNTFIIDGVYGEQGFVSEGNHPGAREGSVMWKDGTGNLWLFGGDADTTTGV
                     NGYKNDLWKYNSTTGQWIWMKGDSTKDNTGVYGTINITASTNKPG"
BASE COUNT          924 a          539 c          770 g          952 t
ORIGIN      
        1 tgccgggctt ctatttgcat gctcaggtat tgttttggct cagcctgtag aagttgactt
       61 atttcaaaag gtagcaacag ctaccacttc ccaaatagga gtaagagtga gggcaactgg
      121 tgccaacatt gattatatag gtgtaacatt ctatattctt taccaaagtg caaatgcagt
      181 accacaatca gctggtgtga atacagttgt tggtgtagat gatagcagga tggtgacaac
      241 ttttgcatgg gggagtagtg caagatttac aaacccagca caggttgtgt ctatcgaccc
      301 aggtgtgcct ggaggacaga cttatgatag aagatacgtt tatgggaata ttgatgaaac
      361 cgggggatca aatatcagga cattgactac tgcctgggat actttgttat acattacctt
      421 aaatacttta caaccaacct ttcctcaggg tgggcatggc tatatccaaa gcacagcaga
      481 ggcagccggg gcggctttat cagacccagg tttttcgaat gttgcttttt tagtcaatag
      541 tggtgacagg cctcttggcc tttctactct tccggtcacc tttaccaaat atgatgtaac
      601 ctgtaatgat aagggtgcat tacttacctg gtctactggt tcagaacaaa acagcagcaa
      661 gtttgaaata cagagaagtt tgaatggtat tgactggatt tctattgata atgtagcagc
      721 agccggtaac agcactgatc agcggaatta tcaatacctt gacctgtatg gtggcactgc
      781 attttatcgt atccgccagg tagatatcga tggcaggttt gtatatactg ccgtgaagcg
      841 gacggaatgc cagaaaagcc agtatgatgt ggcgctgtat cctgttcctg caagggataa
      901 cctgaatgta gttatcaggt ctgaaataaa cacccgtacc gaaattcaga tacttgatat
      961 taacggcaga tcgctgcgcc gcgtagcagt tcagatcaat aagggtaata ataatattca
     1021 attaagtgta ggaaatctcc cggctggcca atacatgctg gtgggtagcg atccttccat
     1081 ccaaatcaat aagaagttta cgattatcag gtaatctata tacttgcctt tttgaaattg
     1141 cctttaccat cacggtaaag gcaattttat ttttatgtat acctgtttgc ttactacatc
     1201 tcttttaact acgccatagc tgacgtttaa attttcaata ccgggacagt tattctgcta
     1261 aacacaattg atttactctt ttcaggtatt ggttttctgg ctgattcttt gtatgtttaa
     1321 taacttatcc tttaaaaaac tactacctcc gttctcctat attgatagcc aattaacagg
     1381 acattatact gccaggtaat tggtccttac tttacccttt cgaaacatct gttccaatca
     1441 gcctttgcta aaacaccacg aacatcacga tgctcatgtg agcatgagta ttatttcatc
     1501 aatgactttg ttttaattcg gttaaatgag aaaaatcttt ctactgttga atttttgttt
     1561 tatcgcattt ttttcaatag ctcaaatagg catcaatact ggtacacctt gggagttttt
     1621 gaaaggagat tctcttgttc cgcaaagtgc accggtttat ggcaatttag gtactgaggc
     1681 tgaaggaaac aggcctggta gaagagctaa ggcttcaacc tggatagatg gttcagggta
     1741 cttatggctc tatggtggta gtgatgcaac attcggaggc agttctgatt tatggaagta
     1801 tgatcccaca aaaaatcagt ggatatttat aaaaggtaat aactcgagaa atgttgcggg
     1861 tgtatatgga ataaaaggtg tttctgatat tagaaataac cctggagcag ggagttactc
     1921 tgctacttgg atagataata caggtaattt ttggttgttt ggaggggtca ataaaaatca
     1981 cttgtggagg tataatccgg ttatcaatga gtggacatgg atgaaaggtg ataatagcgg
     2041 agttgccccc ggggtgtatg gcatacaggg aattgctgcc aatcaaaata atcccggtgt
     2101 caggtcaggc tgtgtatcat ggaaagatgc aactggtaac ttatggcttt ttggaggaag
     2161 tggtctcggt gctagtggaa gttcagggaa tttaaatgat ctgtggaaat ataacccgat
     2221 taccaatcat tggacatgga tgaaaggaga tagtacagtg gggagtttgg gtgtatatgg
     2281 ttctcttgga gtgccgcatc cggataataa accgcctgca aggaatagat ccaattcatg
     2341 ggtggatgcg tctggtaatt tttggttgtt tggagggtgg gcattcgaac ctccgggcac
     2401 aactaagtat tttaatgatt tatggaaatt cgacccagtt actaaccaat ggacatggat
     2461 gaaaggagat tctgttatca atcaattagg tatttatggc agtattggcg ttccggcttc
     2521 aacaaacaaa ccgggatcaa ggaatgacgc tacatcgtgg acagatggag ctggtaattt
     2581 ttggttgttt ggagggtttg gatattcagc aagtagtaat ggcaggctaa atgatctgtg
     2641 gaagtataat cctgtgaaca atgaatgggt ttgggtaaaa ggggataata caataaataa
     2701 ttcagttgta tatggtaccc aaggagtctt tgctgttgca aataaacccg gaggaagagg
     2761 agctgcttct tcctgggctg ataattctgg caacctttgg ctctttggag gaattgaaca
     2821 taggggcaat cttgttgaat tatcagatct atggaagtat gatactcttc tcaatcaatg
     2881 ggcatggata aaaggaccaa atactttcat tattgatggt gtttatggag aacagggatt
     2941 tgtttcagaa ggtaatcatc cgggggcccg ggaaggtagt gtgatgtgga aggatggaac
     3001 aggaaattta tggttgtttg gaggagatgc agatacaaca acaggagtga acgggtataa
     3061 aaatgacctg tggaaatata attcaaccac gggtcaatgg atatggatga aaggagatag
     3121 tactaaggac aatacaggtg tgtatggtac tattaatatt actgcttcca ctaataaacc
     3181 cggtt
//
LOCUS       DAORTI010000247         3169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_42218_length_3169_cov_7.733783, whole genome shotgun sequence.
ACCESSION   DAORTI010000247 DAORTI010000000
VERSION     DAORTI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42218_length_3169_cov_7.733783"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="rodA"
                     /locus_tag="PLO70_16755"
     CDS             <1..371
                     /gene="rodA"
                     /locus_tag="PLO70_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQZ76172.1"
                     /translation="NATQTRYDFVPEQRTDFIFCTVGEGFGFLGSTILLGLYLILLFR
                     IVTIAERQRSVFSRCYAYGVASVFFFHIAINVAMTIGLAPVIGIPLPFISYGGTSLLT
                     FTILLFILIRLDADRQMVLR"
     gene            433..1170
                     /locus_tag="PLO70_16760"
     CDS             433..1170
                     /locus_tag="PLO70_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQZ76173.1"
                     /translation="MRIIPLSEGTFTIDKTKLFVPFDESAHDLKERPVGSLLVEIQPF
                     VIVTSKDILLLDTGLGFEKNGTLQLHQNLMDAGINPSEVTKVLLSHLHKDHAGAVGIE
                     REGLGARLSFPNATYYVQRTELEFAFEKGFPSFITGELECLQNTAQVELLEGNGMIDG
                     YISYEISGAHSPQHQVFWIREDEETIFFGGDEAPQLQQMKHRFVAKYDYDGKKAMELR
                     QQWWKKGEEEKWTFLFYHDVKHPLWKF"
     gene            complement(1228..1641)
                     /locus_tag="PLO70_16765"
     CDS             complement(1228..1641)
                     /locus_tag="PLO70_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76174.1"
                     /translation="MKKILFILSLLLITSTIIFAQNDEGDDRIRDKMREYIQLRMRLT
                     RGEAERFTPVFVRYFNEWRNTLKENRTDRLILQQKVVELRLRYRGEFKEIIGDRRSNE
                     VFEHQERFIKELKNLRQERIQNRQGPRNRIRSLPG"
     gene            complement(1652..2440)
                     /locus_tag="PLO70_16770"
     CDS             complement(1652..2440)
                     /locus_tag="PLO70_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76175.1"
                     /translation="MTQKDNILQELNELNSSLASLYSKNIYTVPVGYFEGLADQVLGR
                     IKATEATNATDELVHLSPLLSKLSKKMPYSVPQGYFEGLEESLISSVQVSGQPAQEEL
                     EELSPLLSSLNKEMPYSVPQGYFENLAIKPVIEANKTGIKVIPIGSRKWIRFAAAAII
                     TGIILTAGFFFINQDKVDPNADSHTWVKKSIKKVSTDKLDEFIELTEEEKSVQGTIAS
                     AEKTQDIKELIKDIPENEIQSLLNDTELLDDSNTDEASDDIMMN"
     gene            complement(2477..3031)
                     /locus_tag="PLO70_16775"
     CDS             complement(2477..3031)
                     /locus_tag="PLO70_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQZ76176.1"
                     /translation="MPLTTASDSELLVQFRNPVTKEKAYTSLVRKYQEKLYWHIRRMV
                     VEHEDANDVLQNVLIRVWNGLENFREDSQLYTWLYRIATNECLTYLEQQKKKASVSLS
                     DVETGLSNKIKADKHFDANKLEWKLQLAMQQLPEKQRVVFALRYYEELPYEEMSRVLE
                     TSEGALKASYHHAVKKIEDYMLNH"
BASE COUNT          850 a          631 c          611 g         1077 t
ORIGIN      
        1 taaatgccac acaaacaaga tatgactttg tgccagagca acgtactgac tttatttttt
       61 gtactgtcgg tgaggggttt ggttttctgg gtagtacaat tttgttgggg ctgtatctaa
      121 ttctcctttt ccggatcgtt accattgcag aacgtcagcg aagtgtattc agccgctgtt
      181 atgcctatgg ggtagcatct gtatttttct ttcacattgc aattaatgtg gctatgacaa
      241 tcgggcttgc cccggtaatc ggtatcccac taccttttat cagttatggc ggcacctccc
      301 tgctaacttt tacaattctt ctttttatac tgatacgact ggatgccgac cggcagatgg
      361 tgttaagata aaatctaaac taagcgattg aagagctgtt attgggagtt ggaatttgta
      421 tttttgaatt ttatgagaat cattccactt tctgaaggta cgtttaccat tgacaagaca
      481 aaactatttg ttccttttga tgaatcagcc catgatttaa aggaaagacc tgttggtagt
      541 ttattagtgg agatacaacc ctttgtaatt gttacctcaa aagatatttt actgcttgat
      601 accggccttg gttttgaaaa gaacgggact ttacagttac accaaaacct gatggatgcc
      661 gggatcaatc cttcagaagt aacgaaagta ttattatcac atctgcataa agatcatgca
      721 ggggctgttg gaattgaaag agaaggatta ggcgcaagac tttcatttcc taatgctact
      781 tattatgtgc agcgaacaga actggagttt gcttttgaaa agggatttcc ttcttttatt
      841 acaggggaat tagaatgttt gcaaaatact gcacaggtgg aactgctgga gggtaacggt
      901 atgattgacg gttatatcag ttatgaaatt tcaggggctc attcacctca gcaccaggtg
      961 ttctggataa gagaagatga agaaactatc ttttttggtg gtgatgaagc cccgcaacta
     1021 cagcagatga aacaccgctt tgtagccaaa tatgactacg atggtaaaaa agcaatggaa
     1081 ctccggcaac aatggtggaa aaaaggagag gaggaaaaat ggacatttct tttctaccat
     1141 gatgttaaac acccgctgtg gaagttttaa aattaaagcc ggttcatatg gaaccggctt
     1201 tttaaatact caaacaacct tttttaatta tcctggtaat gatcttattc tgttacgggg
     1261 tccttgccgg ttctgtattc tttcctggcg taggtttttt aattctttaa taaatctttc
     1321 ctggtgttca aaaacttcat tgcttcttct gtcacctatt atttctttaa actcaccccg
     1381 gtaccttaac cttaattcaa ccactttttg ctgtaaaatc aatctgtctg tacggttttc
     1441 ttttagcgta tttctccatt cattgaaata gcgaacgaaa acgggagtga atcgttctgc
     1501 ttcccccctg gtcagccgca ttctgagctg tatatactct ctcattttat cccgtatcct
     1561 gtcatcgcct tcgtcattct gggcaaaaat gatagtggaa gtaataagga gcaaacttaa
     1621 tatgaataat atttttttca tttttttctg attagttcat cattatatca tcagaagctt
     1681 catcggtgtt ggaatcgtct aataactctg tatcatttaa aagactctga atttcatttt
     1741 ccggaatatc ttttatcagt tctttaatat cctgggtttt ttctgcagaa gcaatggttc
     1801 cctgaactga tttttcctct tcagttaact caatgaactc gtcaagttta tccgtactta
     1861 cttttttaat acttttctta acccatgtat gtgagtcagc attgggatca actttgtcct
     1921 ggtttataaa aaagaatcct gcagttaaga ttatcccggt tatgattgct gccgcagcaa
     1981 agcggatcca tttgcggcta cctattggaa taactttgat tccggtttta ttcgcttcaa
     2041 taactggttt gatggccagg ttttcaaaat atccttgtgg tactgaatag ggcatttctt
     2101 tattcaaact actcagtaat ggagaaagtt cttccaattc ttcctgcgcc ggttgcccgc
     2161 taacctgaac ggatgatatc aatgattctt ccagcccttc aaaataaccc tgtggtactg
     2221 aataaggcat ttttttagac aatttgctca gcaaagggga taaatgcacc aattcatcag
     2281 ttgcatttgt tgcctctgtt gctttgatcc tgccaagcac ctggtcagcc aatccctcaa
     2341 aatacccaac cggaacagta tagatattct ttgaataaag gctggccaaa ctgctattca
     2401 gctcattcaa ctcttgcaat atgttgtctt tctgtgtcat tttacgttaa ttcagacgct
     2461 tatgaggctt caaagtttaa tgattcagca tataatcctc aattttttta actgcatgat
     2521 ggtaactggc ttttaatgcg ccttcactgg tttccaatac ccggctcatc tcttcatagg
     2581 gcagttcttc ataatatcgc agagcaaata caactctttg tttttcagga agttgctgca
     2641 tggctaattg taatttccac tctaatttat ttgcatcaaa atgtttgtca gcttttattt
     2701 tgttactcag accggtctcc acatcgctca ggcttacaga agcctttttt ttctgttgct
     2761 ccaggtaggt caggcattcg ttggtggcga tccggtaaag ccaggtatat aactggctgt
     2821 cctctctaaa attttcaagc ccattccaaa cccttatcag cacattttgc agtacgtcat
     2881 tcgcatcttc atgttctacc accattcgtc ggatatgcca gtacaacttt tcctggtatt
     2941 tcctgacaag gcttgtatag gctttttctt tcgtaaccgg gttacgaaac tgaacgagga
     3001 gttcgctatc acttgcagtg gttagaggca ttgacatcgg gctttgggtg gcttagaata
     3061 ttgggaaagt aatatgttta atggctttgc taataaaaat aaaaaagcag gttcaattta
     3121 agaagaacct gccagaatac tttgaaacaa actgttaacc attattctt
//
LOCUS       DAORTI010000248         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_43523_length_3111_cov_5.048102, whole genome shotgun sequence.
ACCESSION   DAORTI010000248 DAORTI010000000
VERSION     DAORTI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43523_length_3111_cov_5.048102"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLO70_16780"
     CDS             complement(<1..222)
                     /locus_tag="PLO70_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HQZ76177.1"
                     /translation="MKKIILSVALFSMTFSSCDILSKLPTGTGITEAEAGQGIKEALG
                     QGLVKAVLNLNKTDGFFGSQFYKVLLPPDA"
     gene            352..930
                     /locus_tag="PLO70_16785"
     CDS             352..930
                     /locus_tag="PLO70_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQZ76178.1"
                     /translation="MIEVLLEKINSFHPLSPELTERVKSVTSSITYPAGSFILKEGQV
                     CNKACLVVKGLARSYYINEDKDITSRFMDEGFIITSWISYYTQQPGNEYIEALEETTL
                     ACINYADIQKMFIDFPEFNIIGRKQAEYGFYLAEQRTQILRKHTAEEKYKFFLNNHPT
                     LLQRVALKHIATYLGMNEETLSRVRSKYHKSH"
     gene            1039..1653
                     /locus_tag="PLO70_16790"
     CDS             1039..1653
                     /locus_tag="PLO70_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76179.1"
                     /translation="MKAVILAIVNLLCFRVIYSQSFQTPDKKSTALSAGYDIGGTLWW
                     DKHSYNEKERRGMILNFFAAFESKKMSCKVMFSQTGEIDIWGVSDDELQTAVAFLFGK
                     QFTGKNSFISFMAGPSYTSVLYRSYQLVVLPAYTTYTTSMAKEKFMGLTCEAKAGTGR
                     WLGRFYTYLAVSLQGNVSKKGSYAGITGGAGIKVHLGKRSAKKE"
     gene            1805..2347
                     /locus_tag="PLO70_16795"
     CDS             1805..2347
                     /locus_tag="PLO70_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76180.1"
                     /translation="MNTITRQSVLTILSFFLLATGCKKDNPQSEQVTVNGQTFGCRVD
                     GVPFISDLWDYGNNIPPVRVDFWYSPVLRTMEVQIIGKKENQYIEVWLASPIVAGRAL
                     LKTTTLPSPAIPHPPNYGLYQKLSPNQTFITNSSIGGYVDILSVDTVTQKIEGRFEFI
                     GTDQTTGKIVTITNGYFKRS"
     gene            2429..2737
                     /locus_tag="PLO70_16800"
     CDS             2429..2737
                     /locus_tag="PLO70_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76181.1"
                     /translation="MKQLFLKHRLLLPLLLQFTFLLSAKAQQDYLYAEKADSLFANLD
                     KSLITTGILYDRAFPLSRFDLYNPATDTVDYEYISQAYYELYQAAYNFNVIFGAGLVK
                     "
     gene            complement(2749..>3111)
                     /locus_tag="PLO70_16805"
     CDS             complement(2749..>3111)
                     /locus_tag="PLO70_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76182.1"
                     /translation="DAIGKMWEHLEVQAALGYKFNYGDPKQKRIGEFKMYFNEVLLGD
                     NNMLVELDGTKLSYVSHSTINFMLTRDPAFNENMYNHVQNQMRRSTLISEVSEKERAK
                     FFRIIRDRINNRKESLKA"
BASE COUNT          967 a          611 c          590 g          943 t
ORIGIN      
        1 tgcatcaggt ggtaataata ctttgtaaaa ctggctgccg aaaaacccat ctgttttatt
       61 caggttcaac acggctttca ctaatccctg cccgagtgct tccttaatac cctggcccgc
      121 ttctgcttca gtaatgcctg taccagttgg gagtttagat aaaatatcac aggaagaaaa
      181 cgtcattgaa aacaaggcaa ctgacaaaat aatttttttc ataccactac tatttgaatg
      241 attatatcga tcattccgtt gccggttgac caatttttaa agtacaataa aggtactgca
      301 atttagccct gaggacgatc agttctattt aaacgttgta tattagataa catgattgag
      361 gtgcttcttg aaaaaataaa cagcttccat cctctcagcc ccgaactgac tgaaagagtg
      421 aagtcggtta caagtagtat tacctaccct gcgggtagtt tcattttaaa agaaggacag
      481 gtctgcaata aagcctgcct ggttgtaaaa ggattggcca ggtcttatta tatcaatgaa
      541 gataaagata tcacttcccg ttttatggat gaaggcttta tcatcacttc ctggatcagt
      601 tattatacac agcagccggg caatgagtat atagaagcac tggaagaaac cacccttgcc
      661 tgtatcaatt atgctgacat tcaaaaaatg tttatcgact ttccggaatt taatataata
      721 ggaaggaaac aggcagaata tgggttttac ctggcagagc aacgaacgca gatactccgt
      781 aaacataccg ctgaagaaaa atataagttc ttccttaaca atcatcctac tcttttacag
      841 cgggtggcat tgaaacatat tgccacctac cttggcatga atgaggaaac attaagccga
      901 gttcgaagca aatatcataa aagtcattga ttgacagccc aggtttgaca attgtcaata
      961 tctataattc attaatcatc tcttgatatt tgtcaatcca aaagaaaaga aacaactata
     1021 attttcataa aacattttat gaaagcagtg atacttgcaa tagtaaatct gttatgtttc
     1081 agggtgattt attcacagtc ttttcaaaca ccagataaga aaagtactgc ccttagcgct
     1141 ggctatgata ttggcggtac attatggtgg gataaacact cctacaatga gaaagaaaga
     1201 agaggcatga tattaaattt ttttgctgcg tttgagagca aaaaaatgag ctgcaaggta
     1261 atgtttagcc agacaggtga aattgatatt tggggtgtaa gcgatgatga gctacaaaca
     1321 gctgttgcat tcctgtttgg taaacagttt accggtaaaa attcttttat ttcatttatg
     1381 gccggccctt cttatacttc ggtcctgtac agaagttatc aactggttgt tctgccggca
     1441 tatactactt acacaaccag tatggcaaaa gagaaattta tgggtctcac ctgcgaggca
     1501 aaagccggta cgggaagatg gcttgggcgg ttttatacat atttagctgt atcacttcaa
     1561 ggcaatgttt caaaaaaagg gagctacgca ggtataacag gcggcgctgg aattaaagtt
     1621 catcttggca aaagatcggc taaaaaagag tgatattttt tatatcccaa agccttttca
     1681 tattataatc aaaaaaatta tgtcttgaca aatgccagga attttctgtt ttccgcatct
     1741 cttgacattt gtcaatccgt tcctcaaaaa catttttcaa cttaacagta taaaattttc
     1801 aggcatgaat acaattacca ggcagtccgt gttaacaata ctaagtttct ttttgctggc
     1861 aaccggatgc aaaaaagata accctcagtc tgaacaggta actgtaaacg gccaaacctt
     1921 tggctgccgg gtggatggtg ttccatttat ttctgatttg tgggactatg ggaataatat
     1981 tccgccagtt agggtagact tttggtatag ccctgtttta agaaccatgg aggtgcaaat
     2041 aattggaaag aaagaaaatc aatatatcga agtttggctt gcaagcccga tagtagcagg
     2101 aagggctcta ttaaagacaa cgactttacc atctcctgcc ataccgcatc ctcctaatta
     2161 tggtctttat caaaaactat cgcctaatca aacatttatt actaactcaa gcatcggagg
     2221 ttatgtagac attctgagtg tggacactgt gactcaaaaa attgaaggca ggtttgagtt
     2281 tatcggaact gatcagacaa caggaaaaat tgtaactatc acaaacggat attttaaaag
     2341 atcttaatta aggttctaaa caactttcct gaaccttttt tgatagcaag aatcatttaa
     2401 ccatatcagt tatctaaaac tacagcttat gaaacagcta tttcttaagc acaggctcct
     2461 attgccactg cttttacaat ttacatttct gctctctgca aaagcacaac aagattattt
     2521 gtatgctgaa aaagcagatt cactctttgc taacctggac aaatcgctca tcaccacagg
     2581 catactttac gacagggcct tcccgctcag ccggtttgat ttgtataacc ccgctaccga
     2641 tacagtggat tatgagtaca tcagccaggc gtattacgaa ttataccagg cggcttataa
     2701 ctttaatgta atctttggag ccggattagt taaatgaaat aacagctttt atgctttgag
     2761 gctctctttc ctgttattga tcctgtcacg gatgattcta aaaaatttgg ctctttcttt
     2821 ttcgcttact tcacttataa gggtagaccg gcgcatttga ttttgcacat gattatacat
     2881 gttttcgttg aaggccggat ccctcgtaag cataaaatta atggtggaat gtgagacgta
     2941 tgacaatttt gtaccgtcaa gttctaccag catattattg tcgcccagta acacttcgtt
     3001 gaagtacatt ttaaattccc cgatgcgttt ttgtttcggg tcaccataat tgaatttata
     3061 ccccaatgct gcctgcacct ccaggtgctc ccacattttc ccgatagcat c
//
LOCUS       DAORTI010000249         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_43717_length_3103_cov_4.768701, whole genome shotgun sequence.
ACCESSION   DAORTI010000249 DAORTI010000000
VERSION     DAORTI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43717_length_3103_cov_4.768701"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1297)
                     /locus_tag="PLO70_16810"
     CDS             complement(<1..>1297)
                     /locus_tag="PLO70_16810"
                     /inference="COORDINATES: protein motif:HMM:NF014250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Piwi domain-containing protein"
                     /protein_id="HQZ76183.1"
                     /translation="DGFYQPTTEQFRVIQSTSNDLLYANGKTGKEPKKDFKSKGPYQL
                     PNKPNNFKFFFIYQASDKAGAVTELYRYLNSGWKDGQFTFPKMQDYIKVPFELDTTKN
                     IEFDRVENAVTTIRNAIKNTDWQPDTLYMALFINPVPKLEKDEGRKNIYYKIKEMLLY
                     EGVVSQVIKSEHLYKFGKPNPVINTYLSHIEVAMLAKLGGVPWRLNRPINNELIVGVG
                     AFYSVTKKTRFVGSAFCFNNEGMFKGFDCFKGNDSISLAGSIREAVGKFIVTNHTASR
                     LIIHFYKDIGKKELEPILKTLHTLGLNIPVIIVTINKTESKELLGFDTKDKTNLMPYS
                     GTIVKVGWSEYLLFNNTRYDATSKPAQKEYHFPVKIALSCTVDRMLDDMNLVEQLIDQ
                     VYQFSRMYWKSTNQQSLPVTIKYPEMVAEIYPYFEHDRLP"
     assembly_gap    1298..1397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1398..1874)
                     /locus_tag="PLO70_16815"
     CDS             complement(<1398..1874)
                     /locus_tag="PLO70_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76184.1"
                     /translation="MQQNLILNIIPFTSAPGKKSFAFYRERKHDSYYPIFKSDLQGLL
                     DDKLPQLELLELEKLYTDFQPAKEGAIQLNIDLSVSYRFANHYYRHLIREYFLGIADI
                     IQQNFTSETEVWFHDKDKSNAKYNQYNQFTIKVQHSRVTNGPELVLSYDGTTKVFAK"
     gene            complement(1874..2887)
                     /locus_tag="PLO70_16820"
     CDS             complement(1874..2887)
                     /locus_tag="PLO70_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HQZ76185.1"
                     /translation="MQLKKELIQQIQSIIATAKDRAIRSVDTERVLMYWQIGKVILDE
                     EQEGKERAGYGEFLIKSLSETLQPQFGSGFSIRQLELCRQFYRSFPITNALRSQFSWT
                     HYRTLIRIENPDKKEFYIAEAAKNNWTARQLERQVNSQLFERLLLSNEVKDVLAVARE
                     EKFPSDAKEIIKDPMVLEFLGLKREAAYYEKDLEAAIITHLQDFLLEIGNGFSFVARQ
                     KRIHIEGDEFSIDLVFYNRLLQCFVVIEIKTTKLTHEDIGQLQMYVNYYDRFEKQDFE
                     NPTIGILLCADKNDAVVKITLPESNKTIIASKYQLYLPTEQQLIDEVKKEIDKLDKDE
                     NNA"
     gene            complement(2919..>3103)
                     /locus_tag="PLO70_16825"
     CDS             complement(2919..>3103)
                     /locus_tag="PLO70_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76186.1"
                     /translation="GERPIKKEQIIILAEILKVDKDEFLTLWLADQLYDIVKDEKLAN
                     EAMKLAVQKINSRGKK"
BASE COUNT          783 a          593 c          571 g         1056 t
ORIGIN      
        1 ggcagcctgt catgctcaaa gtaaggataa atttctgcca ccatttcggg gtatttaatg
       61 gtaaccggta aactctgctg attggtgctt ttccaataca tacggctgaa ctgatatacc
      121 tggtctatca actgttctac taaattcata tcatctaaca ttctatcaac tgtacaggac
      181 aaagctattt ttaccggaaa gtgatattct ttttgagctg gctttgacgt tgcatcgtac
      241 cgggtattat taaataaaag gtattcagac cagccaactt ttacaattgt gccgctgtat
      301 ggcatcagat tggttttatc ctttgtatca aagcccaaga gttctttgct ttcggttttg
      361 ttgatggtaa caattattac aggaatgttt aagcccaatg tatgcagtgt tttcaggatg
      421 ggttctaatt cttttttacc aatatcttta taaaaatgta tgataagcct tgaagcggta
      481 tgattcgtaa caatgaattt gcctacagcc tcccttatgg aaccagcaag gcttatgcta
      541 tcattgcctt taaaacaatc aaagcctttg aacatgccct cattgttaaa gcaaaaggct
      601 gaaccaacga accttgtttt ctttgtaacg gaatagaatg cacctacgcc aacaatcagt
      661 tcgttattga ttggcctgtt gagccgccac ggcacaccac ccagctttgc cagcatggca
      721 acttcaatgt gtgacaggta ggtattgatt acaggattag gttttccgaa tttatacaaa
      781 tgctcacttt ttattacctg cgaaaccacc ccttcgtaca agagcatttc tttgattttg
      841 taatagatgt tcttacggcc ttcgtctttc tccaattttg gtacaggatt tataaataaa
      901 gccatgtaaa gggtatcggg ttgccaatcg gtatttttaa tagcattgcg aatggtggta
      961 actgcatttt ctactctgtc aaactctatg tttttagttg tatctaactc aaaaggaact
     1021 ttgatatagt cctgcatttt ggggaatgta aactgtccgt ctttccagcc gctattgaga
     1081 tacctgtata attctgtaac tgcccctgct ttatctgatg cctggtaaat gaaaaagaat
     1141 ttaaaattgt tgggcttgtt tggtaattga tacggacctt tgcttttaaa atctttcttg
     1201 ggttcttttc ctgttttgcc attggcatac aacaagtcgt tggaagtgga ttggattacc
     1261 ctgaactgct ctgttgtggg ttggtaaaag ccatctgnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnttt tgcaaatact ttggtagtgc catcataaga aagtaccaac
     1441 tcgggaccat tggtaacccg gctgtgctgc actttaattg taaactgatt gtactggtta
     1501 tattttgcat tgcttttgtc tttatcgtga aaccatactt cagtttcgct ggtaaagttt
     1561 tgctgaatga tatcggcaat gcccaaaaag tattctcgta taagatggcg gtaatagtgg
     1621 ttggcaaacc tgtatgaaac tgataaatca atgtttaact gtattgcacc ttcttttgct
     1681 ggctgaaaat cggtgtagag tttttccaat tccagtaatt ctaattgcgg cagtttatca
     1741 tctaacaacc cttgtaaatc gcttttaaaa attgggtaat agctatcatg cttcctttcc
     1801 cgataaaaag cgaatgattt tttgccgggt gcggaagtaa agggtatgat attaagaata
     1861 aggttttgct gcatcaggca ttgttttcgt ctttatctaa tttgtctatt tcttttttca
     1921 cttcatctat taattgctgc tctgtgggca aatagagttg gtatttactg gctatgatag
     1981 ttttgttgct ttccggtaaa gtgattttta ctacggcatc gtttttatcg gcacataata
     2041 aaatgccgat ggtggggttt tcaaaatcct gtttttcaaa acggtcgtaa tagtttacat
     2101 acatctgcaa ctggccaata tcttcatggg ttagcttggt agttttaatt tcaatcacta
     2161 caaagcattg cagtaacctg ttgtaaaaaa caaggtcaat agaaaattca tcaccttcaa
     2221 tgtgtatgcg tttttgcctt gccacaaaag aaaagccgtt gcctatctcc agcaaaaaat
     2281 cctgcaagtg ggtaatgatg gccgcctcta aatccttctc gtaataagcg gcttctcttt
     2341 ttaagcccag gaactctaac accatcgggt ctttgattat ctctttggca tcggatggaa
     2401 atttttcttc tctggccact gctagcacat cttttacttc attgcttaat aaaagccttt
     2461 caaataactg gctatttacc tgcctttcca gttgccttgc cgtccagttg ttttttgcgg
     2521 cttcggctat gtaaaactcc tttttatccg ggttttcaat acgaataagg gtacggtagt
     2581 gtgtccaact gaattgcgaa cgcagtgcgt tcgtaattgg aaaactgcgg taaaactggc
     2641 ggcatagttc taactggcgg attgaaaagc cactgccaaa ctgaggttgt agtgtttcgg
     2701 acaacgattt tatgaggaac tcgccatagc cagccctttc tttaccttcc tgctcttcat
     2761 ctaaaataac tttaccaatt tgccaataca tcaacaccct ttcggtgtca accgaacgga
     2821 tagctctgtc ttttgcagta gcaatgatgg actgtatttg ctgtatgagt tcttttttta
     2881 actgcacttt atatgattta gttcgtgtta ttcaaaagtt actttttccc tctactattt
     2941 attttttgca ctgctagttt cattgcttca ttagctaatt tttcatcttt cactatgtca
     3001 tacaattggt cggctaacca aagagttagg aattcatctt tgtccacttt caatatttca
     3061 gctaaaatga taatctgctc tttcttaatt ggccgttcgc ctc
//
LOCUS       DAORTI010000250         3099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_43813_length_3099_cov_5.769382, whole genome shotgun sequence.
ACCESSION   DAORTI010000250 DAORTI010000000
VERSION     DAORTI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43813_length_3099_cov_5.769382"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1485
                     /locus_tag="PLO70_16830"
     CDS             325..1485
                     /locus_tag="PLO70_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQZ76187.1"
                     /translation="MPDILIIDDEKAIRKTLTEILSFEGYKIEEAADGEEGFKKFKEK
                     TYDLVLCDIKMPKLDGIEFLQKAGEVNPDVPVIMISGHGNIETAVEAVKKGAYDFISK
                     PPDLNRLLITIRNAMDKSSLVMETKVLKRKVSRVQEMIGDSAPIRKIKETIEKVAPTD
                     ARILITGENGVGKELVARWIHEKSNRASGPLVEVNCAAIPSELIESELFGHEKGSFTS
                     AIKQRIGKFESANGGSLFLDEIGDMSASAQAKVLRALQEGKITRVGADKDISVDVRVI
                     AATNKDLLKEVEAKNFRLDLYHRLSVILIHVPSLNERRDDIPLLVDQFLTDICSDYGI
                     AKKVIDTDAIKLLQQYNWTGNIRELRNVVERLIILSGKTITVEDISNYVMPK"
     gene            complement(1495..2376)
                     /locus_tag="PLO70_16835"
     CDS             complement(1495..2376)
                     /locus_tag="PLO70_16835"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQZ76188.1"
                     /translation="MQHFSIKDIENLCGIKAHTIRAWEQRYKLCVPKRKDSLHRTYDN
                     DDLRELLRVSFLYHNGHKISKIAGLSQADIEKLVEESYKSNTNNETFIHQLVEAGLDF
                     DKERFEKVINSLVLRIGIENCIKDIFYPFLHRIGLLWMTNHAIPAQEHFASHIIRKKI
                     IVATDGLEKSTPNKTNVLVFTPAGEYHEIPLLTANYFFRKHGNQTVYFGVNVALESLK
                     YYLQYHSPQYLYAHIITYLNNSDLEDYISSLCGSFGGKVVLSGYACKKIEKKFSNLKI
                     LDSLDEMISFSKEIAAT"
     gene            2502..>3099
                     /locus_tag="PLO70_16840"
     CDS             2502..>3099
                     /locus_tag="PLO70_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HQZ76189.1"
                     /translation="MPLQHIFVVYLIGTLIVFLPSFGFAKLFAKAGVPSWKAYIPFYN
                     TWVMQELAQRPKHWVFWQFIPVVGWFISPGIFIEFAKVFGRFSLGAHTAAAIGAPVYF
                     PYLAYKEDPKFIGAEAVKNHKKKSWREWVDAAIFAVVAATLIRTFIFEAYTIPSGSME
                     KTLLINDFLFVSKLSYGPRIPNTPLSVPFVHNYLPFGSS"
BASE COUNT          922 a          575 c          643 g          959 t
ORIGIN      
        1 ccgcagcttt gcctgatgtt tcacgaactg taaaattggt aaaagaagta ctctatttag
       61 tcaaataata gctgtctttc gtaatattac tatttaaaaa aattttttct aaaaccatgc
      121 atcgatttga gatggtattt gcatctttgt aatggttttt cataggatat tggattttaa
      181 aacgaggctg ggtttctacc cggcctctat ttttaaaagg gtttaaataa agccacctgg
      241 ctgatcctga ttaccttgct ttagcttgtt acctgttctg cttcaactca aatcgtttat
      301 ttttatgctt taaaatgaat ctccatgcct gatatattga tcatagatga tgaaaaagca
      361 atccgtaaaa ccctgaccga aatattgtct tttgaaggct ataaaatcga ggaggctgct
      421 gatggggagg aggggtttaa aaagtttaag gaaaagacct atgaccttgt tctttgtgat
      481 attaagatgc ccaaactgga tggcattgag tttttgcaaa aagccggcga ggtgaacccg
      541 gatgttcccg ttattatgat ctctgggcat ggaaatattg aaacagctgt tgaagctgtt
      601 aagaaaggtg cttatgactt tatctcaaaa ccgccggacc tgaaccgtct tttgatcacg
      661 atccggaatg cgatggataa aagcagcctg gttatggaaa caaaagtgct gaaacgtaaa
      721 gtaagccggg ttcaggaaat gatcggagac tctgcgccaa tcaggaaaat aaaggaaacc
      781 attgaaaaag ttgctcctac cgatgcaagg atattaatca caggggaaaa tggcgttggg
      841 aaagagttgg tggcccgttg gatacatgaa aaaagtaacc gggcatccgg acctttagtc
      901 gaagtgaatt gtgcggctat cccttcagaa ttaatcgaaa gtgaattatt tggacatgaa
      961 aaaggttctt ttacttctgc catcaaacaa cggataggta aatttgaatc cgctaacgga
     1021 ggctcacttt ttcttgatga gattggagat atgagtgcaa gtgcccaggc taaagttctg
     1081 cgggcattgc aggaagggaa aatcaccagg gtaggagcag ataaagatat tagtgttgat
     1141 gtaagggtta tagcagctac caataaagac ctgttaaaag aggttgaagc taagaatttc
     1201 aggctcgacc tttatcaccg cctcagtgtt attctcattc atgttccttc tttaaatgaa
     1261 aggagggatg atattccgct actggttgac cagttcctga ctgacatttg ctcagactat
     1321 ggtattgcaa aaaaagtaat agatacagat gctatcaaac tattgcaaca atacaactgg
     1381 acaggaaata tccgggaact ccgcaatgtt gtggagcggc ttattatact ttccggcaaa
     1441 acaattacgg ttgaagatat cagcaattat gtgatgccaa aataaacgtt tgcttcaggt
     1501 tgctgcaatt tctttagaaa aggaaatcat ttcatccagc gaatcaagaa ttttcagatt
     1561 ggagaatttt ttttcaattt ttttacaggc atagcctgaa aggaccactt ttcctccaaa
     1621 tgatccgcaa agcgaggata tataatcttc taaatctgaa ttattcaaat aggttataat
     1681 atgcgcatac aagtattgcg gagagtgata ttgtaaatag tacttcagac tttccagtgc
     1741 cacatttaca ccgaaataaa cagtttgatt accatgtttt cggaaaaaat aattggcggt
     1801 cagaaggggt atctcgtggt actcacctgc cggtgtaaaa accaggacat tagttttatt
     1861 aggggttgac ttttccaggc catcagtagc aacaattatt tttttacgga ttatatgact
     1921 ggcaaagtgt tcctgtgccg gtatagcatg attcgtcatc cacagcagcc cgatacgatg
     1981 gagaaaagga tagaatatat ctttaataca attttcaatc ccaatacgca gcacaagact
     2041 gttaatcacc ttttcaaatc gttctttgtc aaaatccaga cctgcttcca ctaattggtg
     2101 aataaaagtt tcattgttcg tattactttt ataagactct tctaccagct tttcaatatc
     2161 ggcctggctc aaaccggcaa tctttgatat tttatgtcca ttatgataaa gaaaagaaac
     2221 ccgaagcagt tctcttagat catcattatc ataagtccgg tgcaggctgt cttttctttt
     2281 tggtacacaa agcttatatc gttgctccca tgcccgaatg gtatgggctt taataccaca
     2341 gagattttct atgtctttaa tagagaaatg ttgcataaac gctaaactct aaattatcat
     2401 ttattctcct gaatctggct acagaattag caaaactttg aatattaaaa ttttgaagcg
     2461 cagatattta taatactttt gtcagctata aaataaggtt catgccatta caacatattt
     2521 tcgtcgttta cctgatcggg accctgatcg tttttctgcc gtcgttcggg tttgcaaaat
     2581 tatttgcgaa agccggggta ccttcctgga aggcttatat ccccttttat aacacctggg
     2641 tgatgcagga actggctcag cgtcccaaac actgggtctt ctggcagttt ataccggtag
     2701 taggctggtt tatcagcccg ggaatcttta tagaatttgc aaaagtattt ggtcgttttt
     2761 cgttgggagc tcatactgct gcggcaatag gtgcaccagt ttatttccct tacctggcct
     2821 acaaggagga tccaaaattt attggagctg aggctgtaaa aaatcataag aaaaaaagct
     2881 ggagagaatg ggtagatgca gcaatttttg ctgtggtggc ggccacactt atccgcactt
     2941 ttatttttga ggcgtatacc attccttctg gttcgatgga gaaaaccttg cttatcaacg
     3001 attttctttt tgttagtaaa ttaagctatg gcccccggat acctaataca ccgttatctg
     3061 ttccatttgt acataattat cttccatttg gttccagcc
//
LOCUS       DAORTI010000251         3099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_43822_length_3099_cov_3.426413, whole genome shotgun sequence.
ACCESSION   DAORTI010000251 DAORTI010000000
VERSION     DAORTI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43822_length_3099_cov_3.426413"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..>3099
                     /locus_tag="PLO70_16845"
     CDS             354..>3099
                     /locus_tag="PLO70_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76190.1"
                     /translation="MKLPLIFLLIILLFSLQGTSQPNQTRISIADKNWQHGGIFSGKT
                     PQQELLSSRSAYTKKFDAGEGLIEIFFGGPFHYEDKTGNWQDINLNIKKAENEGYSFV
                     NNENVFVSRYSEQTDNGVQIEYQGKVMNFGINTRLVSGNWSPVLTNNGVAESFENSVK
                     YKNLYENIDLSYELTTSFIQHKLVFNHRSSLVSLPQGSDFVTIEEIIELPVNTKLHDD
                     NGVIISGRKTSGNIKVMEGTEVLFTIMGSCIWDATFNGNILEQRHAAEQPGNFKNIET
                     SVSYITNSKIKLSALIPVNWLLAPQRIYPVTFDPTVGVGNQGSFNSSYRYPFNTCRQQ
                     RVSQILFRKSDINAGGTTTTGTITDIAFFQNTAAPISNNNVQVKMQEVSWNVMTTPTL
                     TSSGFTTVYGPSTQNFTSGSNVWRTMNLTSPFTFTNTNNLLIEARFSNSTSTSGCNCD
                     NTTPGGHWGWYNSPYAGHRWAYSTGTAPPPSGNTCNYNNSPEGNPAYGYFIPATKITI
                     NTTGGCVPVTFSSHPSSQTVVAPAQAQFTVAVSGTTPTFQWEMSTNGGASWNNVPAGA
                     PYSGITSNQLSINPTSAPLGNNRYRCRVTNSCTPSPVNSNTAVLTINAAGCNTVVTPA
                     SSPSIPAAGAGGSFNINVTPNTCSWSASETLTWMTITSPLSGTGDGTLNYTVAANTGG
                     PRSGTISVNGQIHTINQLGAAAPTTYTISGRVIENGSVGIPNALISTTPALGSTITDA
                     QGYYTLNVPLAYTGTVRATLAPYTFTPLTHPVSSSNFSNKDFDAMGVSINIQPSSSIY
                     PWQREGDDIIATINVSMGTTTTDWRLKADVYDVNNVLTSVLFPPTTALTYTINTSLAN
                     PFQNQTFKNLCKNGSRVEYIAIYNPNPSITDTIKSTKIIEKKWDKKNIVYWRDPSSPD
                     V"
BASE COUNT          995 a          607 c          589 g          908 t
ORIGIN      
        1 ttatttcatt cttgtgccct gctttttggc tgtatacgga agaaaataaa aaagttagac
       61 ccgtgagaag aagtgctttt ttcatatcca aatagtttat tggtcaaata agccttttat
      121 tacaaagtta tgaaggtgta atgtaagggt ttgataattt cagattcaaa ctggcattta
      181 ataataataa gataagtaaa caacatgata aggagtacta aaaattcctg aaaaggctat
      241 aattcttatg tttttaatct tatatctcta tgatttaaat cagttttttt tatagtttaa
      301 aaaattgtaa tttgttatat tgatctattt acttttcaag ccaaaacttt ttcatgaaac
      361 tgccacttat cttcctacta attattctat tgttctcttt acagggcact tctcaaccta
      421 atcagacaag aatttcaata gccgacaaaa actggcaaca cggcggaata ttttctggta
      481 aaacacctca acaggagctc ctttcttcaa gatctgcata taccaaaaaa tttgatgcag
      541 gtgagggact tatagaaata ttttttggtg gaccttttca ctatgaggat aaaactggaa
      601 attggcaaga catcaatttg aatatcaaga aggcagaaaa tgaaggatat tcatttgtaa
      661 acaacgaaaa tgtatttgtg tcccgatatt cagaacagac agataatggt gtgcaaattg
      721 agtatcaagg aaaagtgatg aattttggga ttaatacccg tttagtctcc ggcaactgga
      781 gtccggtgct aacgaacaat ggagttgcgg agtcttttga aaatagtgtt aagtataaaa
      841 atctatacga aaatattgac ctttcgtatg agcttactac cagttttatt cagcataaac
      901 ttgtttttaa ccatagaagc agcctggtca gcttaccaca gggtagtgat tttgtaacca
      961 ttgaagaaat aattgaacta cccgtcaata ctaagttaca tgacgataat ggagtaataa
     1021 tatctggccg gaaaacatcc ggtaacatta aagtgatgga gggaacagaa gtgcttttta
     1081 caattatggg ctcctgcatt tgggatgcaa cattcaatgg aaatatactt gaacaaaggc
     1141 atgctgctga acaacccggt aattttaaaa atattgaaac tagcgtaagt tatattacta
     1201 atagtaaaat caaattatct gctttaatac ctgttaactg gttattagct ccacaaagaa
     1261 tctaccctgt tacatttgac cctacagttg gcgtcggtaa ccagggcagt tttaactcaa
     1321 gctatcgtta tcccttcaat acctgtcgtc agcaaagggt aagccagatt ctgttccgca
     1381 aatctgatat caatgcaggc ggtacaacaa ctacaggcac catcactgat attgcttttt
     1441 ttcaaaatac agcagctcca atatccaata ataatgtcca ggtgaagatg caggaagtgt
     1501 catggaatgt tatgacaaca ccaacactaa catcttccgg ttttacaact gtttatggcc
     1561 cttcaacaca gaatttcaca tcggggtcta atgtatggag aacaatgaat ttgacatcac
     1621 cttttacttt tactaatacc aataacctgc ttattgaagc gagattctcc aattcaactt
     1681 caacgtcggg ttgtaactgc gacaatacaa cacccggtgg ccattgggga tggtataatt
     1741 caccttatgc agggcatcgt tgggcataca gcaccggtac ggcacctcca cccagcggaa
     1801 atacctgtaa ttataataac agcccagaag gaaatcccgc ttatggttat tttatacctg
     1861 caacaaagat tactatcaat acaaccggtg gctgtgttcc tgttacgttc agtagccatc
     1921 catcatcgca aacagtagta gcaccggcac aggcgcaatt caccgttgcc gtttcgggca
     1981 ctactccaac ttttcaatgg gagatgagta ctaatggcgg agcaagctgg aacaatgtgc
     2041 ctgccggcgc tccttattct ggtataacca gcaatcaact atctatcaac ccaacttctg
     2101 ctcctttagg caataacagg tacaggtgcc gggttacaaa tagttgtact ccttctcctg
     2161 ttaacagcaa tacggctgta ttaactatta atgctgccgg atgtaatacg gtggtaacac
     2221 ctgcttcttc tccatctata ccggcagctg gtgctggtgg atcatttaac attaatgtta
     2281 ctcccaatac ttgttcatgg tctgcatcag aaacactgac atggatgacg atcacttcac
     2341 cgctaagtgg tacaggtgac ggcacgttga attacactgt agcagccaat acaggagggc
     2401 cacgcagtgg gaccatttca gtaaatggcc aaatccatac tataaatcaa ttgggagctg
     2461 cagcaccaac gacctatact atttcgggaa gagtgataga gaatgggagt gttggaattc
     2521 cgaatgctct tatttctacc acacctgcat tggggtcaac gataactgat gcacaaggat
     2581 attatacgct taatgtaccg ctggcttata ccggaacagt aagagctaca ttagctcctt
     2641 atacatttac tcctttaact catcctgtta gttcttccaa tttcagcaat aaggattttg
     2701 atgcgatggg tgtgtctatt aatatacagc caagcagttc tatttatcct tggcaaagag
     2761 aaggagatga tattattgca acgatcaatg tcagcatggg tacaactaca acagactggc
     2821 gtttaaaagc agatgtatat gatgtaaaca atgttttaac cagtgtacta tttcctccca
     2881 ctactgcatt gacttacacg atcaatacat ctcttgcaaa tccatttcaa aatcagacat
     2941 tcaagaattt atgcaagaac ggaagcaggg ttgaatatat agcgatatat aatccgaatc
     3001 cgagtataac ggatactatc aaatcaacta agatcataga gaagaaatgg gataagaaaa
     3061 acatagtata ttggagggat ccaagtagtc ctgatgtaa
//
LOCUS       DAORTI010000252         3091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_43991_length_3091_cov_9.292161, whole genome shotgun sequence.
ACCESSION   DAORTI010000252 DAORTI010000000
VERSION     DAORTI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43991_length_3091_cov_9.292161"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1753
                     /locus_tag="PLO70_16850"
     CDS             83..1753
                     /locus_tag="PLO70_16850"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQZ76191.1"
                     /translation="MLTDIAISQSAPIRHIKEIAASIGISNNDLEYYGKHKAKLPLTL
                     INEEKISQHHLVLVTAITPTPAGEGKTTISVGLTDGLNLVGKKAMAVLREPSLGPVFG
                     LKGGATGGGYSQIIPMEDINLHFTGDFAAIEKANNLLAALIDHNLQNKKRSLQIDPRT
                     IVWKRVMDMNDRALRHIVIGLGGTANGITREDGFNITPASEIMAILCMCKGMDDLKQR
                     LGNIYIGTTFGGEPVFARDLNAVGAMAILLKDAIKPNLVQTLEGNPAILHGGPFASIA
                     QGTNSILATQMGLSLSEYVITEAGFAADLGAEKFFDIKCVNAGLKPKAVVVVATVRAL
                     RHHGGAKKDELTIPSIEKVEKGLANLGKHLENMQLFGMKAVVAINHFPGDTAEEIAAI
                     KSYCKNYNTEAVVCKGFTEGGKGMTELAHAVATLVEKGESNFKPLYDREVSIEEKIHT
                     IATRIYGANAVEFSVKAKTQLKHIKELGFEHMPVCMAKTPKSLSDDEKRIGRPVDFIV
                     KVREFEFAAGAGFVIPILGEMMRMPGLPVIPAAEGIDIDANGVITGLS"
     gene            complement(1769..2650)
                     /gene="ygiD"
                     /locus_tag="PLO70_16855"
     CDS             complement(1769..2650)
                     /gene="ygiD"
                     /locus_tag="PLO70_16855"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HQZ76192.1"
                     /translation="MKRKNFIYTITGGLFAMSGLTTFKRFTDDLEEQDVKMPVLFIGH
                     GSPMNGIENNEFSLQWEKTAKELPVPNAVLVVSAHWLTNGTHITAMDYPKTIHDFGGF
                     PQALFDVHYPAPGNSQLALETKELIKTTDVGLNHDWGLDHGAWTVVRRMYPAANIPVL
                     QLSIDFSKPALYHYNLAKELAALRKKGVLIIGSGNMVHNLRMVAWDKLDQPGFGFDWA
                     IEMHELFKTKIIHRDHQALINYEALGKAAKLAVPTPDHYYPLLYTLGLQEKNEEPVFF
                     NDKLVAGSLNMTSVKFG"
     gene            complement(2777..>3091)
                     /locus_tag="PLO70_16860"
     CDS             complement(2777..>3091)
                     /locus_tag="PLO70_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQZ76193.1"
                     /translation="PRHLGVRVVMVKSFARIHETNLKKQGMLALTFANKEDYEKIQED
                     DAIDVIGLTNFTPGKPLQVVLNHKDGSSETIIANHSYNEQQIEWFKAGGALNVIRSQF
                     AK"
BASE COUNT          911 a          640 c          663 g          877 t
ORIGIN      
        1 atatcaggtc ggtaattgat gtttgtcata ctataatgga gcagggaaaa ctaagttgcg
       61 gaaacttttt atcttgttcg ttatgctaac agacattgcc atctcccaat cagcccctat
      121 ccgccacatc aaagaaattg ccgcatccat tggtatcagc aataatgatc ttgagtatta
      181 tggtaaacat aaagccaagt tgccgttaac actgattaat gaagaaaaga tcagtcagca
      241 tcacctggta ctggtaacag ctattacccc tacccctgcg ggtgaaggca agaccactat
      301 ttcagtggga cttactgatg gtttaaacct ggttggtaaa aaagcgatgg ccgttttaag
      361 ggagccttcc ctgggtcctg tttttggatt aaagggaggt gctaccggag gtggttattc
      421 acagatcatt cctatggaag atatcaacct gcactttacc ggtgattttg cagctattga
      481 aaaagcgaat aatctgctgg cggcattgat tgaccataac ctccagaata aaaaaagaag
      541 cctgcaaatt gaccccagaa ccattgtgtg gaaaagagta atggatatga atgacagggc
      601 tcttcgtcat atcgttatcg gactgggtgg cacggccaac ggcatcaccc gtgaagacgg
      661 tttcaatatt acacctgcat cagagatcat ggctatactt tgtatgtgca aaggaatgga
      721 tgatctcaaa caacgtctag gtaatatcta cattggcact actttcggcg gcgaaccggt
      781 gtttgcccgt gacctgaatg ctgttggagc aatggcaatt ttattgaaag atgctattaa
      841 accaaacctg gtacaaacgc tggaaggtaa tcctgccata ctgcacggcg gaccttttgc
      901 cagtatcgcc cagggaacta attctatact ggcaacacaa atgggtttat ccctcagtga
      961 gtacgttatt actgaagctg gctttgcggc cgaccttggt gcagaaaaat tctttgatat
     1021 taaatgtgtg aatgcaggac taaaaccaaa agcagtagta gtggtggcca cagtaagagc
     1081 attacggcat catggcggtg ctaaaaaaga tgaactaaca attccttcta ttgaaaaagt
     1141 tgaaaaagga ttagcgaatc ttgggaaaca cctggagaat atgcaactgt tcggcatgaa
     1201 agcagtagtg gccatcaatc actttcccgg agatacagca gaagaaatag ctgccataaa
     1261 atcttattgt aaaaattata atacagaggc tgttgtttgt aaaggcttca ctgaaggtgg
     1321 gaaaggcatg actgaattag cgcatgctgt tgctactcta gtagaaaagg gtgaaagtaa
     1381 tttcaaacca ctatacgatc gggaagtttc cattgaagaa aagatacata ccattgctac
     1441 cagaatttat ggcgctaatg ctgtagaatt ctctgtaaaa gccaaaacgc agctaaagca
     1501 tattaaagaa ctgggcttcg agcatatgcc ggtatgtatg gcaaaaacgc ctaagagcct
     1561 gtcggatgat gaaaaacgaa ttggtaggcc ggttgatttt attgtcaagg tccgtgagtt
     1621 tgaatttgca gctggtgctg gttttgtaat tccaatactg ggagaaatga tgcggatgcc
     1681 ggggttaccg gttattccgg ctgccgaagg aatagatata gatgccaatg gtgtcattac
     1741 cgggctcagt taataacagg ggtggtgatc atccgaattt aaccgaggtc atattcaatg
     1801 atcctgctac cagtttatca ttaaagaaaa ccggttcttc attcttttcc tgcaagccaa
     1861 gagtatatag taacggatag taatgatcgg gtgtgggaac agccagcttt gcggctttgc
     1921 ccaacgcttc ataattgatc aaagcctggt gatccctgtg tatgatcttt gttttaaaca
     1981 attcatgcat ttcaatggcc cagtcaaacc caaagcccgg ctgatccagt ttatcccagg
     2041 caaccatcct gagattatgt accatattac cactgccaat gatgagaact ccttttttac
     2101 gcaaggctgc cagctcttta gccaggttat aatgataaag tgcaggctta ctaaaatcaa
     2161 tgctcaattg caagacagga atatttgctg caggatacat tcttctcact actgtccatg
     2221 caccatggtc tagtccccaa tcatggttga gtcccacatc ggttgtttta atcaactctt
     2281 tcgtttctaa agctaattga ctgttgcctg gtgccggata gtgaacatca aacagtgcct
     2341 gcggaaaacc cccaaaatca tgtatagttt ttggataatc cattgcagtg atatgtgttc
     2401 cattggtcaa ccagtgtgca gaaaccacca aaactgcatt agggacaggt aattcttttg
     2461 ccgtcttctc ccattgtaaa ctgaactcat tgttctcaat accattcatc ggcgatccgt
     2521 gaccaataaa aagaacaggc atttttacat cctgctcttc aagatcatca gtaaatcgtt
     2581 taaaagttgt tagaccactc attgcaaaca atcctcctgt tattgtatag ataaagttct
     2641 ttcttttcat gtacagcgta aagatacagc aattatttaa tttcaatgtt taaacattga
     2701 taaaattact aaaggcctca taatcataaa gcccccggta tctgacgggg gctttatgaa
     2761 aattatcagg tgctttttac tttgcaaatt gactgcggat aacattcaaa gccccgccgg
     2821 ctttgaacca ttcgatctgc tgttcattgt aagaatgatt agcaataatg gtttcactgc
     2881 tcccgtcttt atgatttaat acgacctgta atggcttacc tggtgtaaaa ttggtgaggc
     2941 caatcacatc aatcgcatca tcttcctgta tcttctcgta gtcttccttg ttggcaaaag
     3001 tcagtgccag cattccctgc tttttaaggt ttgtttcatg aatgcgggca aaagatttca
     3061 ccatcactac acgaacacca agatgccttg g
//
LOCUS       DAORTI010000253         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_44177_length_3083_cov_5.985799, whole genome shotgun sequence.
ACCESSION   DAORTI010000253 DAORTI010000000
VERSION     DAORTI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44177_length_3083_cov_5.985799"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PLO70_16865"
     CDS             <1..1397
                     /locus_tag="PLO70_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HQZ76194.1"
                     /translation="GKREDATHMQEMKEYNIPEIDLVIVDLYPFEETVASTTDEKAII
                     EKIDIGGPSMLRAAAKNHKDVMVVASKADYTELVSILSTQQGETTLDQRRQFAIKAFD
                     VCTSYDNAISNYFHQLTIATPFNAPEKTMRYGENPHQSGAFYGDLDKLFEQLNGKELS
                     YNNLVDVDAAMQMINEFQNTAPLNPPQGGTSNPDSLNDKKNKTTSELSESKNPSSEGR
                     EAVFAIFKHTNVCGVAQRSSVKESWDAALAGDPESAFGGVLICNGTIDKATAEAINEI
                     FFEVLIAPAFDEDALEILKSKKNRILLKNKQSAELPKQMFRSLLNGVLVQGTDEGNFT
                     EWKEVGGRDCTTTEKADLEFANLVCKHLKSNAIALIKNKQLVGKGCGQTSRIDALRQA
                     VEKAKQFSFDLNGAVMASDAFFPFNDCVKLGHEAGISSFIQPGGSIRDKDSIEYCVEN
                     KLAMVITGMRHFKH"
     gene            1401..2408
                     /locus_tag="PLO70_16870"
     CDS             1401..2408
                     /locus_tag="PLO70_16870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQZ76195.1"
                     /translation="MPRYDIYKTLANTTANIKLLRAFNPFNIHKKGTRAKAIFALAVV
                     CVLWGTTWIASKEGVSHMPALQLAGIRQIIAGVLYVGFFIYRKAIWPKGKEWIPIIVL
                     SFLNFILSNGLSTWGVQYISAGLGSIMGAIFPLWLVVIGLFTAKSKLPRNALIGLLLG
                     FGGVCVIFYPHLKDFLIPDFRLGIILSLIATWTWAFASLYTKQQANKFNPYFGLGLQM
                     VIAGITLVITTEATGLSIPIASIPWKAWAAIAYLVLFGSIITFIAYLYALQNLPTEQA
                     SIYAYINPIVAVMCGSLFFGEHISGYIVVGGLVALLGVFMVNRAFKIIPPPEQPETEG
                     I"
     gene            complement(2405..>3083)
                     /locus_tag="PLO70_16875"
     CDS             complement(2405..>3083)
                     /locus_tag="PLO70_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQZ76196.1"
                     /translation="VLSNKVKDTYDSYEWMDELHQQYNLKPNYFFLVPEKNGQYDKNI
                     LPTNTAMHLLIKQHAEKYTVGLHPSWQSDDDHSLLEKEIKTLENITGKDITKSRQHFI
                     LFTLPDTFRRLLSAGIKEEYSIGYGSINGFRASVASPFYWYDLAKEEQTDLLLHPFCY
                     MEANSFYEQKVSSQEALEEMLHYLNEVRKVNGTLITLWHNTFLGTDPFFAGWREVYQQ
                     FIKEAVR"
BASE COUNT          922 a          561 c          645 g          955 t
ORIGIN      
        1 tgggcaaaag agaagatgct acgcatatgc aggaaatgaa ggaatataat attccagaaa
       61 ttgacttggt gatcgttgac ctttatccgt ttgaagaaac tgtagcttct accactgatg
      121 aaaaagcaat tattgaaaaa atagatatcg gcgggccttc tatgttaaga gctgctgcta
      181 aaaatcataa agatgtgatg gtggtggcca gcaaagcaga ttatactgaa ctggtttcta
      241 tactaagtac acaacagggt gaaacaacgt tggatcaacg ccgccagttt gccatcaaag
      301 catttgatgt ttgtacatca tatgataatg cgatctctaa ttattttcat cagctaacaa
      361 ttgccactcc gtttaatgct cctgaaaaaa caatgcggta tggtgaaaac ccacaccagt
      421 ccggagcttt ttatggcgat ttagataaat tgtttgaaca gttgaatggc aaagagcttt
      481 cgtataataa cctggtggat gtggatgcgg cgatgcagat gatcaacgaa ttccaaaata
      541 cagcccccct aaatccccct caagggggga cttcgaaccc cgatagttta aatgacaaga
      601 aaaataaaac gacttctgaa ttgtcagagt ccaagaaccc ttcttcggag ggcagggagg
      661 ctgtattcgc cattttcaaa cacaccaatg tttgtggtgt tgcacaacgt tcttctgtta
      721 aagaaagctg ggatgcagca ttggctggcg atcctgaaag tgcatttggt ggtgttttga
      781 tttgtaatgg aacgattgat aaagcgaccg ctgaagccat caatgaaatt ttctttgaag
      841 tattgattgc tccggcgttt gatgaagatg ctttggaaat tcttaaatca aagaagaaca
      901 gaattttgct gaagaataaa caatcggcag aattgccaaa gcagatgttc cgctccttat
      961 taaatggtgt gctggtgcaa ggtacagacg aaggcaattt tactgaatgg aaagaagtgg
     1021 gtggcagaga ttgcacgaca acagaaaaag ctgatcttga atttgcgaat cttgtttgca
     1081 agcatttaaa atcaaatgca attgcattga taaaaaataa acaactggtt ggtaaaggct
     1141 gcggacaaac aagccgtatt gatgcattaa gacaagcagt tgaaaaagca aagcaattta
     1201 gttttgatct gaatggtgcc gtgatggcaa gtgatgcatt ctttccgttt aatgattgtg
     1261 tgaagttggg acatgaagca ggcatcagtt ctttcattca acccggcggt tctataagag
     1321 ataaagactc tatagagtat tgtgtagaaa ataaactggc aatggtgatt actggaatga
     1381 gacattttaa acattaatca ttgccccgct acgatatata taaaacctta gccaacacta
     1441 ccgctaacat taaactgttg cgggccttta atccttttaa catacataag aagggaacaa
     1501 gagccaaagc aatttttgca ttagctgttg tctgtgtgtt gtggggcact acctggatag
     1561 catccaaaga aggtgtatcg catatgcctg ctttacaatt ggcgggcata cgacaaatta
     1621 tcgccggcgt tttatatgtc ggttttttta tttaccgcaa agcaatctgg ccaaaaggaa
     1681 aagaatggat tccgataatt gtattaagct ttctgaattt tattttgagc aacggcctca
     1741 gtacatgggg agtacaatat atttccgctg gtcttggttc tatcatggga gctatctttc
     1801 ctttatggtt agtggttatc ggtttgttta ctgctaaatc aaaattgcca agaaatgctt
     1861 tgattggttt actactcggc ttcggcggcg tatgcgttat tttttatccg cacctaaaag
     1921 attttcttat tcctgatttt cgtttaggaa ttattctttc attgattgcc acctggacat
     1981 gggctttcgc cagtttatat accaaacaac aagctaacaa attcaatccc tatttcggtt
     2041 taggcttaca aatggttatt gcaggtatta cattggtaat aacaacagaa gccactggtt
     2101 tgtcaatacc aatagcatct attccgtgga aagcatgggc tgctatcgca tatcttgtgt
     2161 tgtttggctc tatcatcacc tttattgcat acttatatgc gttgcaaaat ttacctaccg
     2221 aacaggcatc catttatgca tatatcaatc ctatcgtagc agtaatgtgt ggctcgttat
     2281 tttttggtga gcatatctca ggatatattg tagttggtgg cttggtagct ttgctgggtg
     2341 tgtttatggt gaacagggct ttcaaaatta ttccaccacc tgagcaacca gaaacggaag
     2401 gtatttagcg aaccgcttct ttgatgaact gctgatacac ttctctccaa ccagcaaaga
     2461 acggatcagt tcctaaaaac gtattgtgcc agagtgtaat taatgtgcca tttacttttc
     2521 ttacttcatt taaataatga agcatttctt ctaatgcttc ttgcgatgat actttctgct
     2581 cataaaaaga attcgcttcc atatagcaga aaggatgaag cagaagatcg gtctgctctt
     2641 cttttgctaa atcataccag taaaacggtg aagcaaccga tgccctgaat ccgttgatgc
     2701 ttccatagcc aattgaatac tcttctttga tacctgctga aagtaaacga cgaaaagtat
     2761 cgggtaatgt gaagagtata aaatgctggc gggattttgt aatgtctttc ccagttatat
     2821 tctccagtgt cttgatttct ttttcaagta aagaatgatc atcatcactt tgccaggaag
     2881 gatgaaggcc aacagtatat ttttccgcat gttgctttat caataagtgc attgcagtat
     2941 tagtcggaag aatattctta tcgtattgtc cattcttttc tggcaccagg aaaaaataat
     3001 taggtttcag gttatattgt tgatgtaatt catccatcca ttcgtaagaa tcataagtat
     3061 cttttacttt attactcaac act
//
LOCUS       DAORTI010000254         3077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_44327_length_3077_cov_5.653541, whole genome shotgun sequence.
ACCESSION   DAORTI010000254 DAORTI010000000
VERSION     DAORTI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44327_length_3077_cov_5.653541"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLO70_16880"
     CDS             complement(<1..1082)
                     /locus_tag="PLO70_16880"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HQZ76197.1"
                     /translation="MQLSKNVLGQIAVQPGLEIPAEKLLELPEKVLQFGTGVLLRGLP
                     DYFIDKANKQGIFNGRVVIVKSTDSDSSAFDKQDGLYTICVRGVENGKTIEENIINAS
                     VSRVLSATIEWKRILECAHNLEMKIILSNTTEVGIQLTDDNIKDEPPVSFPGKLLAFL
                     LERFKAFNGSTESGMVIVPTELITDNGSKLESIVLELAHLNNLDFKFIEWLETHNTFC
                     NSLVDRIVPGKPNATELKKTEDSLGYTDELLTNSEVFRLWAIEGNEKTKEVLSFYKAD
                     DAVVITPDITLFKELKLRLLNGTHSFNCGLAYLAGFNITREAVTDKTFSVFAKNLMYQ
                     EIAKAIPFEIETKVKTDFANNVFERF"
     gene            complement(1085..2128)
                     /locus_tag="PLO70_16885"
     CDS             complement(1085..2128)
                     /locus_tag="PLO70_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQZ76198.1"
                     /translation="MIKRKTKISLLYLAAMMLLQACATAQPAQSVPNKIFVAADGSGD
                     YKTIQAAINSLSDSSATARTIFIKKGTYREKIFIEKHNIIFEGEDRTKTTITQDIARD
                     EWRCDHPDDWGVATMNVNGNDITLLNLTIANDYGFNVKESKVVDCKADTVTGKKTITN
                     GGHQMALRTMKATRLKAVNCHFRAYAGDTVSPWNTIDGMFYFKDCSMEGGVDFYCPRG
                     WAWAENCTFYANTGPASIWHDGSNNKDFKTVLMNCKFKGYDGFMLGRYHREAQFYLVN
                     CEFAKNMKDTPIYRVPSNNTINWGERIYYYNCRRVGGKDFSWYSDNLPAGVNANDINV
                     NWVFGDRWNPTIN"
     gene            complement(2125..>3077)
                     /locus_tag="PLO70_16890"
     CDS             complement(2125..>3077)
                     /locus_tag="PLO70_16890"
                     /inference="COORDINATES: protein motif:HMM:NF036632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQZ76199.1"
                     /translation="RKAYRGILKEFIEIEPNGQVNLKGTVAVSGLGGKPYRDGSYEYY
                     MSEPVITNDSKGMGAFIKCAAEMELNETQSVGKGKTVLLDYYFNNEWKEDATGTPVRW
                     HYTWEDKSNSGYAMLGDIFNRYGVQTKSLLNRPTTSDLSKASIYIIVDPDTEKETEKP
                     NYIDPKLPISLSSWVNNGGVLVLLSNDASNAEFKNFNKLAAKFGIQFNEDSKNRVQND
                     QYEQGAVITSTDNLIFTPNRKLFIKEYSSLQVTSPAVTVLKNGEDNVMAVAKYGKGTV
                     FAFGDPWIYNEYLDGRRLTPGFDNYQAAEEWVKWLIKQTK"
BASE COUNT          850 a          655 c          510 g         1062 t
ORIGIN      
        1 cagaaacgtt cgaaaacatt gtttgcaaaa tctgttttca cctttgtttc aatttcaaaa
       61 ggaatggctt tagcaatttc ttgatacatc aggttctttg caaaaacaga aaaggtttta
      121 tctgttaccg cttctctggt aatattgaac ccagctaaat aggccagacc gcaattgaaa
      181 ctatgcgtac cattcaataa acgaagcttt aattctttga acaaagtaat atcaggtgtt
      241 atcactacgg catcatctgc tttataaaaa gataatactt cttttgtctt ttcatttcct
      301 tcaattgccc atagacggaa cacttcgctg ttagtcagta attcatccgt atatcctaaa
      361 gagtcttcag ttttctttag ttcagttgcg ttaggtttac cgggaacaat cctgtccact
      421 aaagaattgc agaaggtatt atgtgtttcg agccactcaa taaacttgaa gtcaagatta
      481 ttcaggtgag ccagttccaa tacaatgctt tccaatttgg agccattgtc ggtaatcaat
      541 tctgttggca caatcaccat accgctttct gtactaccgt taaaggcttt gaatctttct
      601 aataaaaaag ctagcagctt tcccgggaat gatactggcg gttcgtcttt aatattatca
      661 tctgttaatt gaatgcccac ttcggttgta ttggaaagga tgattttcat ttccagatta
      721 tgagcacatt ccaatattct tttccattct atagttgcac ttaatacccg gcttactgac
      781 gcattgataa tattttcttc tattgtcttt ccattttcta caccacgaac acagatagtg
      841 tataagccat cttgtttgtc gaatgcactg ctgtcgctgt cagttgattt tacaatcaca
      901 actcttccgt tgaagatgcc ctgcttgttg gctttgtcaa taaaatagtc gggtaaccct
      961 ctgagcaata ctccggttcc aaattgtaat actttttctg gcaactccag taatttctct
     1021 gccggtattt ccagtccggg ttgtacagca atttgcccta agacattttt tgataattgc
     1081 atatttaatt gattgttgga ttccagcgat cgccaaaaac ccagttaaca tttatatcat
     1141 ttgcatttac accagcagga agattatcgc tgtaccagct aaaatctttt cctcccacac
     1201 ggcgacagtt ataataataa attctttcac cccagttgat cgtattattt gatggcacac
     1261 gatatatagg agtgtctttc atattctttg caaactcaca gttcaccaaa tagaattgag
     1321 cttctctatg ataacgaccg agcataaaac catcataacc tttgaatttg caattcatca
     1381 gtacagtttt aaaatctttg ttattggagc catcatgcca gatactggcc ggacctgtgt
     1441 ttgcataaaa tgtacaattc tccgcccatg cccagccacg ggggcaataa aaatctacac
     1501 caccttccat gctgcagtct ttaaaataaa acataccgtc tatggtattc catggactca
     1561 cggtatcacc tgcatatgct ctaaaatggc agttaactgc ttttagcctt gtagctttca
     1621 tcgttcttaa tgccatctgg tgaccgccat tggtaattgt ttttttaccg gttacagtat
     1681 cagctttaca atcaacaacc ttactctctt ttacattgaa accataatca tttgcaatgg
     1741 tgagatttaa aagtgtaata tcatttccat tcacattcat tgtggctaca ccccaatcat
     1801 cgggatgatc gcaacgccat tcatctcttg caatatcctg tgtgatggta gttttggttc
     1861 tatcttctcc ttcaaaaatg atattatgct tttcgatgaa gatcttttcc ctgtaagttc
     1921 cttttttaat aaaaattgtt ctggctgttg ccgatgaatc agataaacta ttaatagcag
     1981 cttgtatcgt tttataatca ccgctgccat cagccgctac aaaaattttg ttgggtacgg
     2041 attgtgctgg ttgagcagtt gcacaggcct gcaacagcat catagcagct aaatacaata
     2101 acgatatttt ggtttttctt tttatcattt tgtttgtttg atcaaccatt tcacccattc
     2161 ttcagcagct tgataattat caaacccggg tgtaagcctt ctaccatcca ggtattcatt
     2221 atatatccag ggatcgccaa aagcaaacac tgttcctttg ccatatttag caacagccat
     2281 tacattatct tctccattct ttaaaactgt aaccgcaggg ctggttacct gtaagctgct
     2341 atattctttt ataaataatt ttctatttgg agtaaatatt aaattatctg ttgaagtgat
     2401 aacagcacct tgttcatatt ggtcattttg aacacggttc ttactgtctt cattaaactg
     2461 aataccaaat ttagcagcca gcttattaaa gtttttaaat tcggcattgc tggcatcgtt
     2521 actcagcagc actaaaacac caccattgtt tacccaagag ctaaggctga taggcagttt
     2581 agggtctata taatttggtt tttcagtttc tttttctgtg tcggggtcaa ctataatata
     2641 aatggaggct ttgctcaggt ctgaagtagt tggtctgtta agaagcgatt ttgtttgtac
     2701 accatacctg ttaaaaatat cgccaagcat agcatagcca ctattgcttt tatcttccca
     2761 tgtataatgc cagcgaacag gtgtgccggt tgcatcttcc ttccattcgt tgttaaaata
     2821 ataatctagt aaaacagttt tccctttacc aacagattgt gtttcgttca attccatttc
     2881 ggcagcgcat ttaataaaag cacccattcc tttggaatca tttgtgataa ccggctcact
     2941 catataatat tcatagctac catcacggta aggtttgcca ccgagacctg aaacagcaac
     3001 agtgcctttc aggttcactt gtccgttagg ttctatttct atgaattctt ttaatatgcc
     3061 acgatatgct tttcttg
//
LOCUS       DAORTI010000255         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_44450_length_3072_cov_5.100762, whole genome shotgun sequence.
ACCESSION   DAORTI010000255 DAORTI010000000
VERSION     DAORTI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44450_length_3072_cov_5.100762"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1131
                     /locus_tag="PLO70_16895"
     CDS             37..1131
                     /locus_tag="PLO70_16895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ76200.1"
                     /translation="MRSANKFYIILFFLGSFIPANAQQKNYVAINVKVDSFINLQMVQ
                     KGIAGLSLAVMNEGRIIHSKGFGYSNLEHKVPVTNNTVFLMASVTKTFVAVATMMLVE
                     KGDISLTDTIGKFVPDIPSHWKVVTIQQLLSHTSGIKGSIDWPPPCKTELKYDNFNYT
                     RADVINETACLPLSFPPGEKWEYSGRGYYVLGMLIESVSGKTFEQFLKDNIFEPLQMS
                     NTRMIDYKTILANRATGYQKENNKFINKIIPQDPIVEFSDGGLVSTVTDMAKWDAALY
                     SDKLLKKETLQLMFTPVKIKDGYGPYGLGFGTTPFQGHKRVGHIGRIPGFECAFTRFI
                     DKKISVIIFINTELDSGQMDMANQIASFYF"
     gene            1481..1861
                     /locus_tag="PLO70_16900"
     CDS             1481..1861
                     /locus_tag="PLO70_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQZ76201.1"
                     /translation="MKQKTVKLLYWATTLLFAGFMVWSSVPGVSPKGQTLQVLHDYLG
                     YPVYFISFISVAKLIGSAAILVPGFDKIKEWAYAGLFFDLFGAIFSVVYVSKKFDPGM
                     LFILLPVVLGALSYYFWNRKKSFQ"
     gene            1858..2619
                     /locus_tag="PLO70_16905"
     CDS             1858..2619
                     /locus_tag="PLO70_16905"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQZ76202.1"
                     /translation="MKNAAITTVDDYIAGFPPPVRKLLSAIRKTIKAAAPKAEELISY
                     QMPAYKYKGVLVYFAAFEKHIGFYATPTGHETFNKELANYKTGKGSVQFPITEPLPLD
                     LVKRIVQFRVKENEARAAGKSVALKSKPAKLTDEEQVKAWLSKLDPPIKKEIEAVRQI
                     IRNSAAKLHERIKWNAPSYYYKEDIITFGPYQKDGMILLVLHHPAVVKIKSALLQGDY
                     KDRRLVYFNNSKEAAKNKKELARIISEIIKTIDKK"
     gene            2638..>3072
                     /locus_tag="PLO70_16910"
     CDS             2638..>3072
                     /locus_tag="PLO70_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQZ76203.1"
                     /translation="MIKVTFSATIHAPKQRVWYVLWEKSFYEQWTSLFTEGSTVKTDG
                     WKEGTKVQFLGPKESGMLSIVAVNRSYEFMSFRHIGMINQGVEDTESEAVKAWAGAME
                     NYTLKEEAGTTTLTIEMETTEEYKDYFNTTWPKALDKVKELSE"
BASE COUNT         1024 a          561 c          618 g          869 t
ORIGIN      
        1 cgttgtgcgt catgtataaa atagcatcta atctaaatga gatcagctaa taaattttat
       61 attattctat tttttttagg ctcatttata cccgcaaatg cccagcaaaa aaattatgta
      121 gccattaatg taaaagtgga ttcattcatc aatttgcaga tggtacagaa gggtatcgcc
      181 ggattgtctt tagcagttat gaatgaagga agaattattc actcaaaagg cttcggctat
      241 tctaacctcg aacataaagt cccggtaact aataatactg ttttccttat ggcatctgtg
      301 acaaaaactt ttgttgcagt ggcaaccatg atgctggtgg agaaagggga tatttctttg
      361 actgatacta ttggcaaatt tgttcctgac attccttcac attggaaagt tgttactata
      421 cagcaactct taagtcatac atctggaata aaaggaagta tcgattggcc acctccctgt
      481 aaaacagaat taaaatatga caattttaat tataccagag ccgacgttat taatgaaaca
      541 gcctgcttac cgctaagttt tcctccgggt gaaaaatggg aatacagcgg aagaggttat
      601 tatgtattgg ggatgcttat agaaagcgtt tcaggaaaga catttgaaca atttttaaag
      661 gataatatct ttgaaccgtt gcaaatgagt aacacaagaa tgatagatta caagactatc
      721 cttgcaaacc gggcaacagg gtatcaaaaa gagaacaata aatttataaa taaaataata
      781 ccgcaagacc cgattgttga attttcggat ggcgggcttg tatcaactgt taccgacatg
      841 gcgaaatggg atgcggcttt atactctgat aaacttttga aaaaggaaac acttcaatta
      901 atgtttacac ccgtcaagat caaagacggt tatggtccat atggtttagg ttttgggact
      961 accccatttc aaggacacaa acgggttggt catataggaa gaataccggg atttgagtgt
     1021 gcctttacaa gatttattga taaaaaaatc agtgtaatca tttttataaa tactgagctt
     1081 gattctggac aaatggatat ggctaatcaa atagcatcat tttatttttg atttaaaata
     1141 aaaatattaa ggtcaatttt tggattgtct gtaacacgaa cgcccaactt gaactgtgta
     1201 aaaaaccaag cgaaaatttt acttttcaaa aggtttagct gaacagttat caggttgata
     1261 taaactgtgc ctcatagata atacctcaca aataatcttc acccccttat tttagttggg
     1321 cctttagtat tttaatctct tcttcaagca gttgtatctt attccataat acctgggtag
     1381 cagaaaagga tatcccggca aggctgcttt tttccttatt gcctgtttct taaaaaagaa
     1441 atagctttaa atgtaaacaa caaaccaaca tagtcagaat atgaaacaaa aaaccgtcaa
     1501 actcctttac tgggcaacta ccttgctgtt tgcaggcttt atggtctggt catctgtccc
     1561 gggggtttca cccaaagggc aaacattaca ggtgctgcat gattacttag gttatcctgt
     1621 gtatttcatt tcattcatca gtgtggccaa attaattggt tcagcagcta tcctcgttcc
     1681 gggattcgat aaaataaaag agtgggccta tgccggtttg ttctttgacc tgttcggtgc
     1741 cattttctct gtagtatatg tgtcaaaaaa gtttgaccct ggtatgctgt tcatattgtt
     1801 gccagtggtg cttggagctt tatcctatta tttctggaac aggaaaaaat cctttcaatg
     1861 aagaacgctg ctatcactac ggttgatgat tatatcgctg gttttccgcc gccggtaaga
     1921 aaattattat cggctatacg gaaaacgatc aaagctgctg cacccaaagc tgaagagctg
     1981 atcagttacc agatgccggc ttacaaatac aagggggtgc tggtttattt tgctgctttt
     2041 gaaaaacata ttggctttta tgccacccct accggtcatg aaacattcaa taaagaatta
     2101 gcaaactata aaacaggtaa gggctctgta cagtttccga taacagaacc gttaccactt
     2161 gacctggtaa aaagaatcgt tcagttcagg gtaaaagaga atgaagcaag agccgcaggt
     2221 aaatccgttg ccctaaaatc aaaacctgca aaacttaccg atgaagaaca ggtgaaggcc
     2281 tggttgagta aattagatcc cccgataaaa aaggaaatag aagcagtcag acaaataatc
     2341 aggaacagtg ctgcaaaact tcatgaacgg atcaaatgga atgcacctag ttactattat
     2401 aaagaagata ttatcacctt tggcccttac caaaaagatg gaatgatctt gttagtgtta
     2461 catcatccgg ctgtggtcaa aataaaatct gccttgttgc agggtgatta caaagacaga
     2521 aggctggtgt atttcaataa cagcaaagaa gctgcaaaga acaaaaagga actggcccgc
     2581 attatcagcg aaataatcaa aacgattgat aagaaataat tacaaaaaac ttactatatg
     2641 ataaaagtca ctttctctgc taccatccat gcacccaaac aaagagtctg gtatgtgttg
     2701 tgggaaaaat cattttacga acaatggacc agtcttttta cagaaggctc cacggtaaaa
     2761 acagatggct ggaaagaggg caccaaagtg cagttcctcg gtccgaaaga aagtggtatg
     2821 ctgagcattg tggccgtgaa cagatcttat gaattcatgt ctttccgcca cataggtatg
     2881 ataaaccagg gtgtggaaga caccgaaagt gaagcggtga aagcctgggc cggtgctatg
     2941 gaaaattata cactaaaaga agaagccggc actactacgc tgaccataga aatggaaacc
     3001 accgaagagt acaaagatta tttcaataca acctggccca aagcattgga taaagtaaaa
     3061 gaattgtccg aa
//
LOCUS       DAORTI010000256         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_44978_length_3049_cov_5.432532, whole genome shotgun sequence.
ACCESSION   DAORTI010000256 DAORTI010000000
VERSION     DAORTI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44978_length_3049_cov_5.432532"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLO70_16915"
     CDS             <1..383
                     /locus_tag="PLO70_16915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ76204.1"
                     /translation="PAILQQELSDPGNIYHILYHGRQAAGYSKIIFHAPHPGIQQQNI
                     TKLERIYLLKEFYPLKLGYELLRFNIDLSKKNNQAGMWLYVWKENHRAVNFYTKAGFN
                     ITGSYDFKLSETHSNPNHRMLLLY"
     gene            484..996
                     /locus_tag="PLO70_16920"
     CDS             484..996
                     /locus_tag="PLO70_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76205.1"
                     /translation="MKKFTKISLITGLILVAYGYLCRAINIYFFWDSKVIGWFFLFFA
                     LAGLLFSLYRSRKSEGKKTGWVKIGIGFVLLGLIISPIVIFIIKTSDAYQAAIEYLKT
                     DTEIKNTVGNIRGFGLIPTGQVEFSTTNGVESGNAIFNITIRGDKKNKDIVIALEKTP
                     ETMWTVVALE"
     gene            complement(1043..1342)
                     /locus_tag="PLO70_16925"
     CDS             complement(1043..1342)
                     /locus_tag="PLO70_16925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ76206.1"
                     /translation="MPDACLVSPTSPSAGFPYCVETTRGDSSRKKVSAVCNHPDYRGR
                     GYAKALMLWVMQLILENSFTPFLHVLSSNTGAIQLYESIGYRIRKQFFMDVVKRL"
     gene            1366..1659
                     /locus_tag="PLO70_16930"
     CDS             1366..1659
                     /locus_tag="PLO70_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76207.1"
                     /translation="MKLVVIPKNKKQEKALRNFLEESAIDFTIAAEDAAIYKTTHSKK
                     VATKQTDASSEKSPAGVPEWQKQFVRKSIKKYNTHPELLISEKEAWAIIESSK"
     gene            1656..1955
                     /locus_tag="PLO70_16935"
     CDS             1656..1955
                     /locus_tag="PLO70_16935"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQZ76208.1"
                     /translation="MKTFRLFLTPAATAHIIQIAAWYNEQQKGLGTRFKNSLKTELAA
                     IKKNPFSRSFRYDNVRFAVVKKFPYAAHYTIDEDKDMIVIHAIFGFSQSPGKWKR"
     gene            2020..2883
                     /locus_tag="PLO70_16940"
     CDS             2020..2883
                     /locus_tag="PLO70_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ76209.1"
                     /translation="MKTIPVRHITAAHKEQSNPGRFSIRELQPLTKGKDLIHDLHKHD
                     FYFVLAVQKGKGIHEIDFVQYKIHDNTVFILRPGQVHRLELKAGAAGFLLEFDLAFYQ
                     PKNTITEQRWRKASGKNYCAVEAARFMKLHSFLANIFKEISAMQEGYSEAIKASLDLF
                     FIEYIRQSRNPKSIATIKSSYTQERFEELVRLLETNIGHMKNVSQYAGLLNLSPYQLN
                     AIAKQSAGKTVSELINEQIILETKRYLLATSGQVKDIADHLGYEDVSYFIRFFKKQTG
                     HSPEAFRKNFK"
BASE COUNT          994 a          644 c          593 g          818 t
ORIGIN      
        1 cccctgccat actgcaacag gaactcagcg acccgggcaa tatctatcat attctttatc
       61 atggccggca ggcggccggt tattccaaga tcattttcca tgcacctcat cctggtatcc
      121 agcaacaaaa cattaccaag ctggaaagga tctacctgct aaaagaattt tatccgttaa
      181 agctgggata tgaactgctc cgtttcaata ttgatctgtc caaaaaaaac aaccaggcgg
      241 gtatgtggct ttatgtgtgg aaagaaaacc accgggcggt aaacttttat acaaaggcag
      301 gcttcaatat tactggcagt tatgatttca aactcagcga aacacattcc aatcccaacc
      361 accggatgct gttgctgtat taaacttgcg ctgcagtact ttattttgaa ggtgtttgta
      421 accagaagac tccttgactg agcctagtcc ggacgtattt tgtaattacc tatctttaaa
      481 cacatgaaaa aattcacaaa gatttcattg attacaggat taatactggt agcttatggc
      541 tatttgtgcc gtgccataaa tatttatttc ttctgggaca gcaaggtaat cggatggttc
      601 tttttatttt ttgctctggc aggcttgctg ttcagtctgt acaggtcaag aaaatcagaa
      661 gggaaaaaaa ccggctgggt gaaaattggt atcggtttcg tattacttgg gttgatcatt
      721 tcaccaatag ttattttcat tatcaaaact tctgatgcct accaggctgc aattgaatat
      781 ctcaaaacgg atacggaaat caaaaatact gttggtaata tcaggggttt tggcctgata
      841 ccaactggtc aggttgaatt cagcactact aatggagtgg aatcgggtaa tgcaattttc
      901 aatataacaa taaggggtga taaaaaaaat aaagatattg ttatagcgtt ggaaaagact
      961 ccggaaacaa tgtggactgt tgttgcgctt gaataatcca aaaccaggta cattttacca
     1021 ctcataaggt tacaggaatt cctcacaatc ttttcaccac atccataaaa aattgtttgc
     1081 ggatgcggta gccgatagat tcatatagtt gaatcgcccc ggtattgctg ctgagaacat
     1141 gtaaaaaagg agtaaaagaa ttttccagta tcagttgcat cacccacaac attaatgctt
     1201 ttgcataacc cctgcccctg taatcaggat gattacaaac ggcgcttacc ttcttccttg
     1261 acgagtctcc ccgggtagtt tctacgcaat aaggaaaacc ggcggaggga ctcgtcggtg
     1321 aaacaagaca ggcgtccgga atttaaaaaa atgtactttt atttaatgaa actggtagta
     1381 atccccaaaa acaaaaaaca ggaaaaagct ctgcgtaact tcctggaaga atctgcaatt
     1441 gatttcacca ttgccgcaga agatgctgcc atatataaaa caactcactc aaaaaaagta
     1501 gctaccaaac aaactgatgc ttcttcggaa aagagtcctg ccggcgtccc tgaatggcag
     1561 aaacaatttg tacgcaaaag cataaaaaaa tacaacaccc atccggagtt gctcatttct
     1621 gaaaaagagg catgggctat tattgaaagt agcaaatgaa gacgttcagg cttttcctca
     1681 ctccagctgc cacagcacat atcattcaga tagcagcctg gtacaacgag cagcaaaaag
     1741 gtctaggcac ccgttttaaa aacagcttaa aaactgagct ggccgctata aagaaaaatc
     1801 cttttagccg ctctttccgg tatgataatg tgcgttttgc agttgtaaaa aaatttccat
     1861 acgctgccca ctacactatt gatgaggata aggacatgat tgtaatccat gctatatttg
     1921 gcttcagtca aagtcccggg aaatggaaaa gatagtttac ataaacacag tggaaacttt
     1981 acaggtccaa aaccaccaat ctcagtaact taattctaaa tgaagaccat tcccgtcaga
     2041 catattactg ctgcccacaa ggaacaaagc aatccgggcc ggttcagcat ccgggagttg
     2101 cagccattaa ctaaagggaa agaccttatt catgatcttc ataaacacga cttctatttt
     2161 gtgctggccg tacaaaaagg caagggcatc cacgaaatag attttgtaca atacaaaata
     2221 cacgataata cagttttcat ccttcgcccc gggcaggttc accggttgga attaaaagcc
     2281 ggcgctgcgg gtttcctgct ggagtttgat ttggcttttt accagccgaa gaataccatc
     2341 accgaacagc gatggagaaa agcatcaggc aaaaactatt gtgccgtaga agcagcaagg
     2401 tttatgaaac tgcactcctt cctcgctaat atattcaaag aaatttctgc aatgcaggaa
     2461 ggttattcag aagcgataaa agcgagtctc gatcttttct ttatagaata tatccgtcaa
     2521 agccggaacc cgaaaagtat tgccactata aaaagcagtt acacccagga acgttttgaa
     2581 gaactggttc gtttactgga aacgaatatc gggcacatga aaaatgtgtc tcaatatgcc
     2641 ggcctgttaa acctttctcc ctaccagcta aatgccattg ccaaacaatc tgcaggaaaa
     2701 acagtgtctg aacttatcaa tgaacaaatc atactcgaaa caaaaagata tttgcttgct
     2761 acttccggcc aggtaaaaga catagccgat cacttgggtt atgaagatgt ttcctacttt
     2821 atccggttct ttaaaaaaca aaccgggcat tctcctgagg cattccggaa gaattttaaa
     2881 taagtcctgt tcaactgcaa aatcgtcctg tcaaaccacc atttatacaa tctatttttg
     2941 tactgataaa atatttgatc aaataaaaca aatcagcatg aaataagcca taaaattttc
     3001 gagctattat accaaccggc gatgaaaatt ttatggcgaa taacatgtg
//
LOCUS       DAORTI010000257         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_45240_length_3038_cov_4.836742, whole genome shotgun sequence.
ACCESSION   DAORTI010000257 DAORTI010000000
VERSION     DAORTI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45240_length_3038_cov_4.836742"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1405)
                     /locus_tag="PLO70_16945"
     CDS             complement(110..1405)
                     /locus_tag="PLO70_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQZ76210.1"
                     /translation="MPASNRRKFLQRAGIFSATAFLSSLGQPAWSRNLETVLKVAEGI
                     SPEDLATEEDFWYYIQQSFTVSPSLINLNNGGVSPAPKTVQDAMKRFYDYSNEAPSYY
                     MWRILDQGREPLRKNLANVAGCSPEEIAINRNSSEGLETVIFGLQLKAGDEVVASLQD
                     YPNMINAYKQREMRDGIKMKWINLELPSEDEDYLVRQYVNAFTAKTKLVHITHIINWN
                     GQILPVKKIANEAHKRGIEVIVDGAHSFAHFNFKIPDLDCDYFAASLHKWLYAPIGTG
                     VLYVKKEKIKTIYPLFATNENPLKDDIRKFENLGTRPFFIEQAIGKAIEFHEMIGIER
                     KEKRLHFLKNYWIEKVKDIPGVKVNTSLHPKWGCAIGNVGIEGKKPNELDSFLMTNYK
                     IHTVAITWENIVGVRITPNVYTTTKNLDVLVEGIKAFVK"
     gene            1799..>3038
                     /locus_tag="PLO70_16950"
     CDS             1799..>3038
                     /locus_tag="PLO70_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQZ76211.1"
                     /translation="MKVLEIKVLKGPNYWSIRRPKLIQMKLDLENLEQKPTNLIPGFR
                     ERLEKMFPTMIEHRCSEAVRGGFFMRVDEGTWMGHVIEHIALEIQTLAGMDTGFGRTR
                     TPEGEQEGVYYVVFSYMEEDAGVFAAKSAVRIAEALIAGKEYDLEADIQEMREIREDT
                     RLGPSTGCIVEEAAKRGIPYIRLNKHSLVQLGYGVNQKRIRATIASTTSNIAVDIACD
                     KEETKLLLEAAEIPVPRGTVIRTVEGMDAAIEKFGYPLVIKPIDGNHGKGNTTNIINR
                     EQALQAFAAAKEYGRSVIIEKFITGFDFRCLVINNKFICAALRTPASVVGDGQHNIQW
                     LIDETNKDPRRGFGHEKVLTQITIDQFTQKMLDDAKYTLETVPAKGERVMLKPTANLS
                     TGGTSTDVTDEVHPANIFMFE"
BASE COUNT          953 a          551 c          625 g          909 t
ORIGIN      
        1 tcattcaaga gggtttgata aagaacaaat gtaatagaaa aataacttac aggtactagg
       61 aacaacggca gttgtggata tgtggaaaac aggcttcgtg agattgatac tatttcacaa
      121 acgccttaat cccttcaacc agcacatcca gattttttgt agtagtatac acattcgggg
      181 taattcttac acccacaata ttttcccagg taatggcgac tgtatgtatt ttataattag
      241 tcattaagaa tgaatccagt tcattcggtt tttttccttc aatacctaca ttgccgatgg
      301 cacagcccca tttaggatgt agagaagtat tcacttttac accgggtata tcttttactt
      361 tttctatcca gtaattcttc agaaaatgta atcgtttttc ttttctttca ataccaatca
      421 tttcatgaaa ctcgatagct ttaccaatcg cctgttcaat aaaaaatggc cgggttccaa
      481 ggttttcaaa tttgcggata tcatctttca acgggttttc attggtggca aataagggat
      541 agattgtttt tatcttttcc ttctttacat ataaaactcc tgtgccgatg ggcgcataca
      601 accatttatg caaactggcg gcaaaataat cgcaatccag atcaggaatc ttgaaattaa
      661 aatgagcaaa gctgtgtgca ccatccacaa tcacttctat ccctctttta tgtgcctcgt
      721 ttgcaatttt tttaactgga agaatttgac cattccaatt gatgatatga gtgatgtgaa
      781 ccagttttgt tttagctgta aaagcattta catattggcg aactaaataa tcttcgtctt
      841 cacttggtaa ttcaagattt atccatttca ttttaatacc atcccgcatt tctctttgct
      901 tgtaggcatt gatcatattg ggataatcct gcaacgaagc caccacttca tcaccagctt
      961 ttagctgtaa accgaaaatg actgtttcta atccttcgga tgaatttcta ttgatagcaa
     1021 tttcttctgg tgaacaaccg gctacattcg ccaaattttt tcttaatggt tctctgccct
     1081 gatccaaaat ccgccacata taataactcg gtgcttcgtt ggaataatca taaaatcttt
     1141 tcatggcatc ttgcacagtt ttgggtgcgg gggaaacacc accgttgttc agatttatta
     1201 atgaaggtga aacagtaaat gattgctgga tataatacca gaaatcttct tcagttgcca
     1261 aatcttctgg tgaaatgcct tcggctactt ttaatacagt ttccagattt ctgctccatg
     1321 ctggctggcc taatgaagaa aggaatgctg ttgcggaaaa aattcctgcc cgttgcaaaa
     1381 attttcggcg gttagaagct ggcatggtgg tttgttttta taaatgtagg gaatttaaac
     1441 ccaccccaat ttcaataaag tataagtatt ccttcaattc gcccctccaa ggaggggaag
     1501 tttccgacta tattgctaat gagttttttg attgattaaa agtcttgaaa tcattttaag
     1561 tttttcaaaa gcatggactg tgcatagcat cccctccgaa agcggtttgg ggtgcttgcc
     1621 tgccggtagg caggggttgc tcagtagaat tccaacagct gaagtgtgat tcccttttat
     1681 tctttcactg gaagaataaa agggaacaaa tcatagattg caagggacga tgagaagaat
     1741 tacaaatgtt ggctacaaaa aataaaaaaa ctaacttcca accttaacat taaccaatat
     1801 gaaagtatta gagattaaag tgcttaaagg cccgaattac tggagtatcc gtcgtcctaa
     1861 attgattcag atgaaactgg accttgagaa tctggaacaa aaaccaacga acctgattcc
     1921 tggtttcaga gaacggcttg aaaaaatgtt tccaacaatg attgagcatc gctgtagtga
     1981 agctgttcgt ggcggatttt ttatgcgggt agatgagggc acatggatgg gccatgtgat
     2041 tgaacatatt gcattggaga ttcaaacact tgcaggaatg gataccggat ttggaagaac
     2101 aagaacaccg gaaggtgaac aagagggtgt atactatgta gtgttcagct atatggaaga
     2161 agatgcaggt gtgtttgccg caaaatctgc tgtgagaatt gcagaagcat tgattgctgg
     2221 aaaggaatac gatttggaag cggacattca ggagatgcgg gaaataagag aagatactag
     2281 gcttggacca tctacaggtt gcattgtaga agaagcagcg aagagaggaa taccttatat
     2341 aagattgaac aaacacagtc ttgtacaact tggctatggt gtaaatcaaa aaagaataag
     2401 ggcaacaatt gcttctacca cttcaaatat tgctgttgat attgcctgcg ataaggaaga
     2461 aacaaaatta ttattggaag ctgccgaaat acctgtacca agaggaacgg tgatacgtac
     2521 agtagaaggt atggatgcag ctattgaaaa gtttggttat ccgctggtga taaaacccat
     2581 tgatgggaat catggaaaag gaaatactac caatatcatt aaccgtgaac aggcattaca
     2641 agcttttgcc gctgcgaaag aatatggccg gagtgttatc atagaaaaat ttataactgg
     2701 atttgatttc cgttgtttgg tcatcaataa taaatttatc tgtgcagcat taagaactcc
     2761 ggcttctgtt gtgggcgatg gtcaacataa tattcaatgg ctgattgatg aaacgaataa
     2821 agatccacgt cgtggatttg gacatgaaaa agttttgaca caaataacta ttgaccagtt
     2881 tacacagaaa atgctcgacg atgccaaata cactttagaa actgttcctg ccaaaggaga
     2941 aagggtaatg ttgaaaccaa cagcgaatct ttcaaccgga ggaacatcaa ccgatgtaac
     3001 agatgaagtg catccggcga atatttttat gtttgaac
//
LOCUS       DAORTI010000258         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_46374_length_2990_cov_6.882794, whole genome shotgun sequence.
ACCESSION   DAORTI010000258 DAORTI010000000
VERSION     DAORTI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46374_length_2990_cov_6.882794"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLO70_16955"
     CDS             <1..1416
                     /locus_tag="PLO70_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQZ76212.1"
                     /translation="EWIIANALAEEHELDEIAAKAKEYVRESRAAAWAKFITPVKKQV
                     VRATDLVHAMANVLPEHTEALHKIAIELSANREPLRRDVLKALNAALDIAGVTDAAFW
                     TRDYYKDLLEENNKLYDSHLYNEGPKSALKVEEVKAVITSESPLLNGFEVLNRYFDEL
                     FTANPKVIAFGEDLGYIGDVNQGFSGLQEKHGTERIFDTGIRELTIMGQGIGLALRGL
                     RPIAEIQYLDYLLYGLQPLSDDAATVHYRTAGQQSCPVIVRTRGHRLEGIWHSGSPMG
                     MIINALRGMYVCVPRNMTQAVGMYNTLLRSNDPGLVIECLNGYRLKEKLPSNLLEFTV
                     PLGIPEIIKEGTDITIVSYGSTLRIIQDAVNILEAKGISCEIIDVQTLLPFDIHHIIL
                     SSLKKTNRIVFVDEDVPGGAAAYMFNKVMEEQGGYKFLDVAPRTITGKAHRPAYGSDG
                     DYFSKPNAEEIQAVIKEMMAE"
     gene            1472..1702
                     /locus_tag="PLO70_16960"
     CDS             1472..1702
                     /locus_tag="PLO70_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQZ76213.1"
                     /translation="MAIVKVIEVIASSEKSFDDAVKQAVKETSKTVRNIDSVWVKDMK
                     AHVKGGKIKSYGVICKVSFRIDKKDKEKESEV"
     gene            1777..2403
                     /locus_tag="PLO70_16965"
     CDS             1777..2403
                     /locus_tag="PLO70_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HQZ76214.1"
                     /translation="MPTHFLHIIDILGTIAFAVSGAFLAMEKKLDPFGVLVLAFVTAI
                     GGGTLRDILIGNLPVSWLRNETASIVIFSAAVVTMLFGRYLKKFTTTLFLFDALGLGL
                     FTLVGIKLGIEKDFSVGICITLGTITACFGGVVRDVLLNNIPLLFRKEIYAMACIAGG
                     AVYFLLREMNIDPDLSTILGILIIFVIRIIAVRYKLFLPQFYTSTIQK"
     gene            2400..>2990
                     /locus_tag="PLO70_16970"
     CDS             2400..>2990
                     /locus_tag="PLO70_16970"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQZ76215.1"
                     /translation="MKMTIYQVDAFANKLFGGNPAAVIPLKEWLDTELMQKIALENNL
                     SETVFFVPSEKKSVDYDIRWFTPAVEIDLCGHATLASAYIVFNILKEKKRKIVFSSQS
                     GLLIVKKEKDRMEMDFPSWKPERVNDYPAVLSSVLNGAEIVGVYKHRDLLVELLNEEA
                     VKNCKPDFSAMKRHFDKMIITAPGKEVDFVSRFFAPG"
BASE COUNT          896 a          515 c          703 g          876 t
ORIGIN      
        1 gaatggatca tcgccaatgc attggcagag gaacatgaac ttgacgaaat agctgcaaaa
       61 gcaaaagaat atgtaagaga aagcagggca gcggcctggg caaaatttat tacgcctgtt
      121 aagaagcaag tagtcagagc aacagacctc gttcatgcaa tggcgaatgt attgcctgag
      181 catacagaag ccttacataa gattgctatt gaattgtctg ccaatcgtga accattacga
      241 agagatgttc tgaaagcgtt gaatgcagca ctggatatag caggcgttac tgatgcggct
      301 ttctggacaa gggactatta caaagatttg ctggaagaaa acaataaatt gtatgattct
      361 catctttata atgaggggcc aaagagtgct ttaaaggtag aagaagtaaa agctgttatt
      421 accagtgagt ctcctttgct gaatggtttt gaagtactca acaggtattt tgatgagttg
      481 tttactgcca atcccaaagt gatcgcattt ggtgaagacc ttggctatat tggtgatgtg
      541 aaccagggtt tcagcggatt gcaggagaaa catggaactg aaaggatctt tgatacaggt
      601 atcagggagc tcacaattat gggacaggga atcgggcttg cgctgcgtgg gttaagaccc
      661 attgctgaaa tacaatatct tgattactta ttatatggtt tgcaaccatt aagcgatgac
      721 gctgcaactg ttcattacag aaccgctggt cagcaaagtt gtcccgttat tgttcgtacc
      781 aggggccacc gcctggaagg gatctggcat agtggttcac caatgggcat gatcatcaat
      841 gcacttcggg gtatgtatgt atgtgtgccg agaaatatga cccaggctgt aggtatgtat
      901 aatactttat tacgcagtaa tgatccgggt cttgtaatag agtgcctgaa cgggtaccgg
      961 cttaaagaga aactgccttc caacttattg gagtttaccg taccgttagg tatcccggaa
     1021 attataaaag aaggaacgga tatcaccatt gtttcttacg gatcaacatt gcgcatcatt
     1081 caggatgccg tcaatatact tgaagcaaaa gggatcagct gtgagatcat tgatgtgcag
     1141 acactattgc cttttgatat tcatcatatc atactttcat cactcaaaaa gacaaaccgc
     1201 attgtatttg tggatgaaga tgtacccgga ggtgctgcag catacatgtt taataaagta
     1261 atggaagaac agggtggata taaattcctg gatgtagcac ccagaactat tacagggaag
     1321 gctcatcgtc cggcttacgg cagcgatgga gattatttca gcaaaccaaa tgcagaagag
     1381 atacaggcgg tcattaagga aatgatggca gaataaaaat tagttgtacc ttatatgggt
     1441 aaaatgtatt ttaaccatca aaccgtttgc tatggccatt gtaaaagtta ttgaagttat
     1501 cgcttcctca gaaaaaagtt tcgatgatgc cgtaaaacag gctgtaaagg aaacttccaa
     1561 aactgttcgt aatattgact ctgtgtgggt aaaggatatg aaggcgcatg taaaaggagg
     1621 gaaaataaaa tcgtatgggg tgatctgtaa ggtgtcattc cggatcgata aaaaggataa
     1681 agaaaaagag agcgaagtat aaattatttc cgggaagtaa atgcctgtta atcctgtctt
     1741 gtttattgca gacaggattt ttaattctat aagaccatgc ccacacactt tctgcatata
     1801 atcgatatac tgggtaccat tgcatttgcc gtatccggtg ctttcctggc tatggaaaaa
     1861 aagctggatc cttttggcgt gttagttttg gcctttgtta cagcaatcgg cgggggcaca
     1921 ttaagagata tactaattgg taatctcccg gtaagctggt taagaaacga aacagcctct
     1981 attgtaattt tttcagcagc ggttgtgact atgctttttg gccgttacct gaagaagttt
     2041 accactactt tatttctctt tgatgcatta gggcttggtt tatttacatt ggtaggtatc
     2101 aaattaggga ttgaaaagga tttcagtgtt ggtatttgca ttaccctggg aactataact
     2161 gcctgttttg ggggtgttgt aagggatgtg ttacttaata atattccttt attatttcgc
     2221 aaagagattt atgcaatggc ctgtattgca gggggcgctg tttattttct gctgcgggaa
     2281 atgaatatag atcctgattt gtcaaccatt ctcggcatcc tcattatttt tgtcatccgg
     2341 ataatagcag tccggtataa actgtttctg cctcaatttt atacttcaac tattcaaaaa
     2401 tgaaaatgac aatttaccag gtggatgctt ttgccaataa gttatttggc ggcaatcctg
     2461 cagcggtgat tccgttaaaa gaatggctgg atacagaact gatgcaaaaa atagccctcg
     2521 aaaataatct tagcgaaact gtcttctttg ttccgtctga aaagaagagt gttgactatg
     2581 atatccgttg gtttacacct gcagtggaaa tcgatctatg tggtcatgcc acactggctt
     2641 ccgcctatat tgtttttaat atcctgaaag agaagaaaag gaaaattgtt tttagttcac
     2701 aaagcgggtt actgattgtt aaaaaggaaa aagataggat ggaaatggat tttccatcct
     2761 ggaagcctga acgggtaaac gattatcccg ctgtcctttc atctgttcta aatggcgctg
     2821 aaatcgtagg ggtgtataaa caccgtgatt tgctggtgga actgttaaat gaagaagcag
     2881 ttaagaactg taaacctgat ttttctgcta tgaaaaggca ctttgataaa atgatcatca
     2941 ctgcgccagg aaaagaagtg gactttgtgt caaggttttt tgcacccgga
//
LOCUS       DAORTI010000259         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_47747_length_2937_cov_5.223109, whole genome shotgun sequence.
ACCESSION   DAORTI010000259 DAORTI010000000
VERSION     DAORTI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47747_length_2937_cov_5.223109"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLO70_16975"
     CDS             complement(<1..683)
                     /locus_tag="PLO70_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQZ76216.1"
                     /translation="MYSTKTNFELSYKRLPSMKNGKITLALVDDHQIVIDGLMSLLKG
                     HDKFRFAFATTDSSEVVQKLAETPVDILLTDVMMPKLPGNELAKKVKQHFPGVRILAL
                     SMSGQGDLVNEMINDADISGYVLKNIGKQELIKALEKIASGGIYFSEEVIDELQRTSQ
                     RKKQKEEAHLTDREIEIIRLIEKEYNNKQIAEELFISERTVETHRKNIFRKTNTNSVI
                     GLVKYAYEH"
     gene            complement(803..2614)
                     /locus_tag="PLO70_16980"
     CDS             complement(803..2614)
                     /locus_tag="PLO70_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQZ76217.1"
                     /translation="MKRISFLLIAICISSFIRAQVNVDSLHAIIGSKRSYNDQINASL
                     KLLENFQLKNFDTTLIEGNKALQLARKNTDSVSVAEIKRHIGVANYFKGRYDEAAKNF
                     YESIAILEKKNEKKKLAPVYNELAKLYRKTRDLDRSLENYNKASAIYNELKDTSGISM
                     ILNESGVVYEYREDYKEAINRYTASLKLAEAAGDSLSVSYSLSNIAGVYVIQKEYDKA
                     EANHLRALSIRQLLKDSFAIALTFSDLGATMNAKGDYTKAVKYLTLSNRFAEGLKYPE
                     LQSNNYSELSSIAQKQGDFQNAFQYFLKRSALRDSLYALEKTKQIAELNTRYETAQKE
                     QQIQNQKNKLRLQNFLFIGIAGLVLLAGLLVYSQYKRYRLRKETQLQAEIMKQQEIAV
                     KAVLEAEENERQRIARDLHDGVGQMMSAAKMNLSAFESAIRFTDAEQKDSFEKIIALV
                     DESCKEVRTVSHIMMPNALLKNNLAAAIRELVDKLNNKALKIHVYTEGLDERLDSNME
                     TVLYRVIQECVTNAIKHAGATTLDISLIKDKEGISGTIEDNGKGFDTTDKEKFEGIGL
                     KNITTRIEYLKGTVDFDSAPGRGTVVALFVPLQAGSA"
     gene            complement(2622..>2937)
                     /locus_tag="PLO70_16985"
     CDS             complement(2622..>2937)
                     /locus_tag="PLO70_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQZ76218.1"
                     /translation="HHTQKKDAPSGTAITLAEQILQEVKRKKQWVNELSDHPDDLEIM
                     SHRIDPAPGTHSVKYSSVIDDIEIIHTAHSRTGFATGAVLAAEFIKDKKGFFTMKDVL
                     NL"
BASE COUNT          674 a          638 c          637 g          988 t
ORIGIN      
        1 ttatgctcat aagcatactt taccaggcca atgacagagt tggtatttgt tttccggaag
       61 atatttttgc ggtgtgtttc tacggtacgt tcggagataa ataattcctc ggctatttgt
      121 ttgttgttgt attctttttc tatcagccgg atgatctcta tttcccggtc cgttagatgg
      181 gcttcttctt tttgtttttt acgctggctg gttcgctgca gttcatcgat cacttcttca
      241 ctgaaataga tgcccccgga tgcaattttt tccagggctt tgatgagttc ctgtttgccg
      301 atattcttta atacataacc ggaaatgtcg gcatcattga tcatttcatt cacgaggtcg
      361 ccttgtccgc tcattgaaag ggccagtatc cttaccccgg gaaagtgttg ctttactttt
      421 ttcgccagtt cattgcccgg cagctttggc atcattacat cggtcagtaa aatatcaact
      481 ggagtttcag ccagtttttg caccacttcg gatgaatcgg tggtggcaaa agcaaaacgg
      541 aatttgtcat ggccttttag caggctcatc agtccgtcaa ttacaatttg gtggtcatca
      601 accagggcca gtgtgatctt tccgtttttc attgatggta atcgcttgta agatagctca
      661 aaattggttt tggtgctgta cataacagat aatttactac ccggatgcag caaacggatg
      721 aattactgtt tgagatttgt taaagaatga aaaagctcca tgaacaaggg gtaggggact
      781 tcgcaaaaaa ggacaacctc tattaagcag agcctgcctg caggggaaca aacaaggcca
      841 ctacggtgcc gcggccgggg gcagaatcaa agtctacagt gcctttcagg tattcaatac
      901 gggtggtgat atttttgagc ccgatgcctt caaatttttc tttatccgtg gtatcaaagc
      961 cttttccgtt gtcttcgatg gtaccgctga tgccttcttt atcttttatc agggagatat
     1021 cgagagtagt ggcaccggcg tgtttgatgg cattggtcac acattcctga atcacccggt
     1081 acagcaccgt ttccatattc gagtccagtc tttcatccag tccttcagtg tacacatgta
     1141 ttttaagtgc cttattattg agcttatcta ctaattcacg gatagcagca gcgaggttat
     1201 ttttgagcag ggcattcggc atcataatat gcgacaccgt acgtacctct ttgcagcttt
     1261 catctaccag tgcaatgatt ttttcaaagg aatccttttg ttctgcatcg gtaaaccgga
     1321 ttgctgattc aaaggccgag agattcatct tggccgcact catcatttgt cccaccccat
     1381 catgcaggtc cctggcgatg cgctgtcgtt cattttcttc cgcttcgagt accgctttca
     1441 cggctatttc ctgctgcttc ataatttcag cctgtagttg tgtttctttt ctcaaccggt
     1501 atcgtttgta ttgggaatag accagtagcc cggccaataa gaccaaaccg gcaatgccaa
     1561 taaacagaaa attttgcagc cggagtttat ttttctggtt ctggatctgc tgttcttttt
     1621 gtgctgtttc gtaccgggta ttcagctctg ctatctgttt tgtcttttca agtgcataca
     1681 ggctgtcacg cagggccgat cgttttaaaa aatactgaaa ggcattctgg aaatcgccct
     1741 gtttttgtgc gatggaagaa agttcagagt agttattcga ttgcaattcc gggtatttca
     1801 atccctctgc aaagcggtta ctcaaggtca ggtattttac tgcttttgta taatcccctt
     1861 ttgcattcat ggtggcaccg agatctgaaa aagtaagggc aatggcaaaa ctgtctttca
     1921 gcagctggcg gatcgataag gcccgcagat gattggcttc cgctttatca tactcttttt
     1981 gtatcacata cactcctgca atattgctga gtgaatagga aacacttaat gaatcaccgg
     2041 ctgcttctgc cagtttcagg gaagccgtgt agcgattgat ggcttcttta taatcttccc
     2101 ggtattcata caccactcca gattcattca atatcatgga aatgccactg gtatctttca
     2161 gttcattgta aatggcactt gctttattat aattttccag tgaccggtca aggtctcttg
     2221 ttttgcggta aagttttgcc agttcattgt aaacaggggc taattttttc ttttcatttt
     2281 tcttttccag gatagcgatg gattcgtaga aattctttgc cgcttcatca taccggcctt
     2341 tgaaatagtt ggctactcct atatgtcttt ttatttctgc cacacttacc gaatcggtat
     2401 tcttccgggc cagttgcagg gctttattgc cttcgatcag ggtggtgtca aaatttttaa
     2461 gttgaaaatt ctccagcaat ttcagcgatg cattgatctg gtcattatag gatcttttgg
     2521 agccgattat ggcatgaagg ctgtccacat ttacctgtgc ccggataaat gaactgatac
     2581 agatcgctat aagtaaaaat gaaatacgct tcataccctt tttataggtt cagtacatct
     2641 ttcatcgtga agaaaccttt tttgtctttt atgaattctg ctgccagtac agcgccggtt
     2701 gcaaaacctg tccggctgtg cgctgtatgt atgatctcaa tatcatcgat cactgatgaa
     2761 tattttacac tgtgtgtgcc gggtgccggg tctatccggt gactcatgat ctccaggtca
     2821 tccgggtgat cactgagttc attcacccat tgtttttttc tttttacttc ctgcagtatt
     2881 tgttcagcaa gtgtaatggc agtaccgctg ggagcatctt ttttttgtgt gtgatgg
//
LOCUS       DAORTI010000260         2879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_49347_length_2879_cov_7.632436, whole genome shotgun sequence.
ACCESSION   DAORTI010000260 DAORTI010000000
VERSION     DAORTI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49347_length_2879_cov_7.632436"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLO70_16990"
     CDS             <1..408
                     /locus_tag="PLO70_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQZ76219.1"
                     /translation="AETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNVFIV
                     CAIGATLFLGGWMPFHIGNWTAFNHVMDYIPSSVWFFGKTFFLIFVIMWFRWTFPRLR
                     IDQLLNLEWKYLLPISMINLLLVTAMAILGWHF"
     gene            464..643
                     /locus_tag="PLO70_16995"
     CDS             464..643
                     /locus_tag="PLO70_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76220.1"
                     /translation="MNWLKIAGYMIAVSGLGILGWNAYSYLTGNIVDKSTAGAGILLC
                     AIGAFLIRRATHKTD"
     gene            657..1133
                     /locus_tag="PLO70_17000"
     CDS             657..1133
                     /locus_tag="PLO70_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQZ76221.1"
                     /translation="MFITKYIKDVFGAIKSLAIGLKRTLYYFTHHKEIITQQYPDNRE
                     TLALPERFKGEVVMPHNENNEHRCTGCQACELACPNGTIKVIFKNELTPEGKKKKAID
                     KLVYHLELCTMCNLCVEACPSDAIKMAQTFEHSVYDRNELTKILNNPGSKIMEGVE"
     gene            1130..1648
                     /locus_tag="PLO70_17005"
     CDS             1130..1648
                     /locus_tag="PLO70_17005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQZ76222.1"
                     /translation="MITGSTILFYLLATLTLGCGILSVTTRHIFRAAIYLLFSLIGIA
                     GLYFWLQYEFIAAVQIVVYVGGIVVLIIFSIFLTQQAGEKLPKQKSDRKIFAALAVFC
                     GFALTMLQVYQHEFNGLETTKAPTVADIGKKMLSVGDGGYALPFEIISMLLLAAMVGC
                     IVIALRSKPETK"
     gene            1645..1959
                     /gene="nuoK"
                     /locus_tag="PLO70_17010"
     CDS             1645..1959
                     /gene="nuoK"
                     /locus_tag="PLO70_17010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQZ76223.1"
                     /translation="MIEPGLYHILFISAALFFLGIYGFLTRRNLITMLMSIELILNSV
                     NLNFIAFNKYVWPDKMDGLFFTLFIIAIAAAEAAVAIAIIINLYRSHQSIDVENAEDL
                     KY"
     gene            1966..>2879
                     /locus_tag="PLO70_17015"
     CDS             1966..>2879
                     /locus_tag="PLO70_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQZ76224.1"
                     /translation="MPNATTIALIPLLPLAGFILLGLFGRKYLKSSSGLIGTALLLTS
                     TILALYTAYEYFFVYGKVDGVYQQFIPLQLTWLEFSKGVSIDMGLLLDPISVMMLVVV
                     SFISLMVHIYSLGYMKGEERFATYYSFLGLFTFSMLGLVLASNIFQIYIFWELVGVSS
                     FLLIGYYYDKPSAVAASKKAFIVTRFADLGFLIGILILSFSSGTLDFTTLIQRLTSPE
                     SNEVKTAVVSTFLGASALTWGAALVFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHA
                     ATMVVAGVYLVARLFPVFALTTPAVLEI"
BASE COUNT          779 a          584 c          626 g          890 t
ORIGIN      
        1 gccgaaacca accgggctcc gtttgatttg gctgaagctg agtcagaatt aacagccggt
       61 ttccatacag aatattcagg aatgaagttc gccttattct tcctggctga atatgtaaat
      121 gtgtttattg tttgtgcgat tggcgctaca ctgtttcttg gtggctggat gccatttcat
      181 attggtaact ggactgcctt taatcatgta atggattata ttccatcgag tgtatggttc
      241 tttggaaaaa cattcttcct catatttgtc atcatgtggt tcaggtggac attcccgaga
      301 ttaaggattg accagctcct gaacctggaa tggaaatatc tcttaccaat cagtatgatc
      361 aatttgttat tggtaacggc gatggcaata ttgggttggc atttttagcc ccggaccccc
      421 caaaagggtg caaggggaag ttcttattta ttaagttaaa aaaatgaact ggcttaaaat
      481 agcaggatat atgatagccg tttcaggcct gggaatcctg ggttggaatg cctattcata
      541 tctcaccggg aatattgttg ataagtcaac agcaggtgca ggaattttac tttgtgcaat
      601 aggtgctttt ctgattagaa gagcaacaca taaaacagat tgaatcgaaa atagaaatgt
      661 ttattacgaa gtacataaaa gatgtttttg gcgccatcaa atcactggca atcggcttga
      721 agcggacgct gtattatttt acacatcata aagaaattat cacccagcaa tatcctgaca
      781 accgtgaaac attggctttg ccggaacggt ttaaaggaga agtggtgatg ccgcataatg
      841 aaaataatga acaccgatgt accggctgcc aggcttgtga attggcctgt cccaatggga
      901 ccattaaagt gatctttaaa aatgagttaa ccccggaagg caagaagaaa aaagcaattg
      961 ataaactagt ctatcacctg gagctttgca ctatgtgtaa cctctgtgtg gaagcctgtc
     1021 cttctgatgc gatcaaaatg gcacagacat ttgaacatag tgtgtatgac agaaacgaat
     1081 tgacaaagat cttaaataac ccgggttcta aaataatgga aggagtagaa tgataacagg
     1141 ttcaactata ttattttatt tgctggcaac cctgacgctg ggttgtggta tcttatccgt
     1201 cacaacaaga catatattcc gtgcggcgat ttacctgttg ttctccctga tcggcatcgc
     1261 tggtctctat ttctggctgc agtacgaatt tattgctgca gtgcagatcg ttgtatatgt
     1321 ggggggtatc gtagtactga tcattttctc tatattcctt acacaacaag ccggagaaaa
     1381 actgcctaaa caaaaatcag acagaaaaat atttgcagca ttagcagtct tctgcggatt
     1441 tgcactgacc atgctgcagg tataccagca tgaattcaat ggcctggaaa caacaaaggc
     1501 ccctacagtg gctgatattg gtaaaaagat gttgagtgtc ggcgatggcg ggtatgcctt
     1561 accttttgaa attatttcga tgctattgct ggctgccatg gttggatgta tcgtaattgc
     1621 cttaagaagt aaaccggaaa caaaatgata gagcccggac tatatcatat tttatttatc
     1681 agtgccgccc tgttcttcct tggtatttat ggttttttga cccgaagaaa cctgatcacc
     1741 atgctgatga gcattgaact gattctgaac agtgtgaacc tgaatttcat tgcctttaat
     1801 aaatatgtat ggccggataa aatggatggc ttgtttttca ctttgttcat cattgccatt
     1861 gccgctgcag aagctgccgt ggccatcgcc atcatcatta atttataccg cagtcatcaa
     1921 tccattgacg tggagaatgc agaagattta aagtattaaa ccaatatgcc caacgcaact
     1981 accatagcac tgatcccatt gttgccgctt gccggattca tattgcttgg tttattcggc
     2041 agaaaatatt taaagagcag ttccgggctt atcggaacag ccctcttact tacttctacc
     2101 atattagccc tgtatactgc atatgagtac ttttttgtct acgggaaagt agatggagtg
     2161 tatcaacaat tcattccgct tcaattaacg tggttggaat tctccaaagg cgtttccatt
     2221 gatatgggcc tgcttctcga ccccatttcc gttatgatgc tggtcgttgt cagttttatt
     2281 tcgctgatgg tacatattta cagccttggc tatatgaaag gtgaagaaag attcgcaacc
     2341 tactattcat ttcttggcct gttcaccttc tctatgctcg gacttgtgct tgcctctaat
     2401 attttccaga tctatatatt ctgggaactg gtaggtgttt cctctttcct gcttattggc
     2461 tattactatg ataaaccaag tgcagttgcg gcctctaaaa aagcatttat cgttacccgg
     2521 tttgcagact taggtttttt aataggcata ctgatattat cctttagttc aggaacactt
     2581 gattttacca cacttattca acgtctgact tcaccggaat ccaatgaagt aaagacagcc
     2641 gttgtttcta cattcctggg tgcatcagca ctgacatggg gagctgcctt agtatttata
     2701 ggtggtgccg gtaagagtgc catgtttccg ctgcatatct ggctacccga tgccatggaa
     2761 ggacccactc ctgtctcagc attgatacat gctgctacca tggtagtggc aggtgtttac
     2821 ctggtagcaa gattattccc ggtttttgcc ctgaccactc ccgctgtgct ggagattat
//
LOCUS       DAORTI010000261         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_49965_length_2857_cov_6.088508, whole genome shotgun sequence.
ACCESSION   DAORTI010000261 DAORTI010000000
VERSION     DAORTI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49965_length_2857_cov_6.088508"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2369
                     /locus_tag="PLO70_17020"
     CDS             <1..2369
                     /locus_tag="PLO70_17020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQZ76225.1"
                     /translation="IDSTNTAVFGTVTDYKNAGNYEFNYVKSKTSNIIELKNTITRQY
                     DIPVGKVQLIRVPYYENVNVTSTLTCLPWDGNKGGVLVLNVQDTINLSSSIDVSGKGF
                     RGGSDPFSNPSSFNCYENQFFYPVNPDLASEKGESIATISSSKSFGKGANANGGGSGN
                     SHNSGGGGGSNSGLGGFGGYNFEADVCSVTVPFDNRGIGGRSLPYNNSANKIFLGGGG
                     GGGHSNNPQAFESKGGSGGGISIIISDKIKSNGNKIIANGNNGLPCGSTGSGCHEGMG
                     GGGSGGTILLNATTFIDNNQIEIKGGNGADMIATGFFKVGPGGGGGGGLLWISNTTLP
                     ANINLSTAGGINGVCTGYSNNPWGTTAGGAGSNLFNLQIPIDNIPFKPNIDSVKIKDS
                     LLSCSNFDFKGLGYTNTNPVVGWEWHFGDGATAYTQNTTHSYVQGNYTVKLIITDING
                     CKDSITKNFSASALTMDAGPADTICASGSTILQASSNGATQYAWTPAAFLNDPTILNP
                     TATPPTTTMFYLTATNASGCSSEDSVLINVRSLNNFSIDAVTPICRNKSVQLNANGGD
                     LYSWLPAISLNNPGIFNPTASPIITTTYSVLITDTICGNASTLSTTVTVLPLPIVTAS
                     RSNDIDCSSSQSQLTASGASQYSWSPAATLSNPNSGSPIAIPGITTQYIVAGTNSNGC
                     INYDSVTVKVSGDNPAGYLMPTAFTPNNDGKNDCYRVKHWGTILELEFSIYNRWGERV
                     FYTKNPNDCWDGRYKGVVQNPAVFVYMIKAKTTCVNEIFRKGTFALIR"
     gene            complement(2372..>2857)
                     /locus_tag="PLO70_17025"
     CDS             complement(2372..>2857)
                     /locus_tag="PLO70_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQZ76226.1"
                     /translation="RLIVRKFTTSDYDNHFLLTGNADVMRYIRPVKNKEESDLYFNET
                     VLDAPAHAFQGRWAVEEKGTKRFIGSFGIIPIPDDDAKTQMGYSFLPAYWGKGLASEL
                     VEAGLIYFKERTPLAEIYGVTEVENVASQKVLLKNGFVPFGKKMDGDIELLVFVFKRE
                     Y"
BASE COUNT          923 a          547 c          541 g          846 t
ORIGIN      
        1 taattgattc tactaatact gcagtttttg gcaccgtaac tgactacaaa aatgctggca
       61 attatgaatt taattatgta aaaagtaaaa ctagcaatat aatagaatta aaaaatacaa
      121 ttacaagaca atatgatatt cctgtaggca aggtccaatt aataagagtt ccgtattacg
      181 aaaatgtaaa cgttacttct acattaacct gtctgccttg ggatggtaat aaaggaggag
      241 ttcttgtttt aaatgtacag gacacaatta atttaagttc cagtattgat gtaagtggca
      301 aaggatttcg tggaggaagc gaccctttct caaatccttc atcatttaat tgctacgaaa
      361 atcagttttt ttatccagta aatccagatc ttgcttcaga gaaaggggaa agtattgcta
      421 caatttcttc atctaaatca tttggcaaag gggcaaatgc taatggaggt ggcagtggga
      481 actcacataa ttccggcggt ggtgggggaa gtaattcagg cctaggtggc ttcggtggat
      541 acaattttga agcggatgta tgtagtgtta ctgttccttt tgataacaga ggcattggag
      601 gaagatcgtt accatataac aattccgcta acaaaatatt tcttggcgga ggaggtggtg
      661 gtggtcatag taataatcct caggccttcg aatccaaagg aggttctggt ggaggtattt
      721 caattattat ttcagataaa ataaaatcaa acggaaataa gataattgct aatgggaata
      781 acggacttcc ttgtggtagt accggatcag gttgccatga aggaatgggc ggtggtggta
      841 gtggtggaac tattttgcta aatgctacta catttattga taataatcag atagaaatta
      901 aaggaggtaa cggagctgat atgattgcta caggtttttt caaagtagga cccggtggtg
      961 gtggtggtgg tggtttatta tggatatcta atacaacatt acctgcgaat ataaatcttt
     1021 ctacagccgg aggaataaat ggagtttgta ccggatatag taataatcca tggggaacaa
     1081 ctgctggcgg ggcaggttca aatttgttca acttgcaaat ccctatcgac aatattcctt
     1141 tcaaaccaaa tatagattct gtaaaaataa aagacagctt actatcctgt agcaattttg
     1201 attttaaagg attgggttat acaaatacaa atccagttgt tggttgggaa tggcattttg
     1261 gtgatggagc tacagcctac acacaaaata ctacacactc ttatgtgcag ggaaattata
     1321 ctgtaaaatt aattattact gatatcaatg gctgtaaaga cagtatcaca aaaaactttt
     1381 ctgcatcagc acttacaatg gatgccgggc ctgcagatac aatctgtgct tctggctcta
     1441 ccatattaca agcaagcagt aatggagcca ctcaatatgc atggacacct gctgcatttt
     1501 taaacgaccc aaccatttta aatccaacag ctactccgcc tactactacg atgttttatc
     1561 ttactgcaac aaatgcaagt ggatgttcta gtgaagattc agtattgata aatgttcgtt
     1621 ctttaaataa tttttccatc gatgctgtaa ctcctatttg tcgcaataaa tccgtacagc
     1681 taaatgcaaa tggcggagac ctctactcct ggctacctgc tatctcgttg aacaatccag
     1741 gtatatttaa tccgacagct tcgccaataa tcaccacaac ttattctgta ctaataacag
     1801 ataccatttg tggcaacgct tctaccctat ctacaaccgt tactgttttg ccattaccaa
     1861 ttgttactgc aagcagatct aatgatatcg attgcagttc ttcgcaaagt cagttaaccg
     1921 caagtggggc ttcgcaatat agttggtcgc ctgcagctac gttaagcaat ccaaattccg
     1981 gaagtccgat cgctatacca ggcatcacta cacaatatat agttgcagga acaaattcca
     2041 atggatgtat aaactatgac agcgttacag taaaagtaag cggcgacaat cctgctggct
     2101 atttaatgcc aacagctttc acaccaaaca acgatggcaa aaatgactgt tacagagtaa
     2161 aacattgggg aacaattctc gaactcgaat tcagcattta caatcgctgg ggcgaaagag
     2221 tattctatac aaaaaatcca aatgattgct gggatggacg atacaaaggt gtagtacaaa
     2281 accctgccgt ttttgtatat atgataaaag caaaaacaac ttgtgtaaat gaaattttcc
     2341 ggaagggaac atttgcattg atcagataat tctaatattc tcgcttgaac acaaacacca
     2401 gcaactcaat atccccatcc atctttttgc caaaaggtac aaatccattt ttcaataata
     2461 ctttttgtga agcaacattt tcaacttccg ttactccata aatttctgcc aatggagttc
     2521 tttctttaaa ataaatcaac cccgcttcta ctaactcgga tgccaaaccc ttaccccaat
     2581 atgctggaag aaaagaatat cccatctgtg tttttgcatc atcatcagga attggaataa
     2641 taccaaacga tccaataaaa cgcttcgttc ctttttcctc aacagcccat cttccttgaa
     2701 aagcatgagc tggtgcatcc aataccgttt cattaaaata aagatcactc tcttccttat
     2761 tttttaccgg ccggatataa cgcattacat cagcattacc agttaataga aaatggttgt
     2821 cgtaatcaga agttgtaaac ttccttacaa ttagtct
//
LOCUS       DAORTI010000262         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_52841_length_2760_cov_5.224399, whole genome shotgun sequence.
ACCESSION   DAORTI010000262 DAORTI010000000
VERSION     DAORTI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52841_length_2760_cov_5.224399"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..116
                     /locus_tag="PLO70_17030"
     tRNA            42..116
                     /locus_tag="PLO70_17030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Val,seq:tac)
     gene            150..410
                     /locus_tag="PLO70_17035"
     CDS             150..410
                     /locus_tag="PLO70_17035"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQZ76227.1"
                     /translation="MWTVYIIYSRIKDRYYTGFTGDSITERIRKHNTRHSGFTGGGSG
                     DWELQYSETFTNKEEASKREREIKSWKSRKKIEKLIGEGHPD"
     gene            777..1271
                     /locus_tag="PLO70_17040"
     CDS             777..1271
                     /locus_tag="PLO70_17040"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HQZ76228.1"
                     /translation="MKKIFSALFLLAGAITILSTAAPGCKAKPKDANIKTSIEAALKS
                     DPQTSGLSVDVKEGVATVTGEVKDASVQATVATIVGGIKGVKSVLNNVTVTPPVVITP
                     DDPLTMAVKDATKDFTGITATVKDGIIYVTGELKSADWKKLKMALDGLKPKKVDAAGL
                     KISK"
     gene            1291..1716
                     /locus_tag="PLO70_17045"
     CDS             1291..1716
                     /locus_tag="PLO70_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQZ76229.1"
                     /translation="MQLKDKYKDLMDAAVASGVNNLQVREQDGVLYIDGEAPSGVVKD
                     QLWETYNKLDPDFLSGDVVMNVNVSPSIPGTKVKVTTERSNLNIRKGPGTDQPIVGKA
                     AHHEIVTLVSQSNDQWWLIRTNDGEEGYAYAQYLTPEPN"
     gene            1774..1977
                     /locus_tag="PLO70_17050"
     CDS             1774..1977
                     /locus_tag="PLO70_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQZ76230.1"
                     /translation="MPIVKVIEVIASSPVSFDDAIKNCLAEVSKTVHNIDSIYVKDFK
                     VHVKEGKITSYGTICKVSFRVDS"
     gene            2231..2533
                     /locus_tag="PLO70_17055"
     CDS             2231..2533
                     /locus_tag="PLO70_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HQZ76231.1"
                     /translation="MKATATNTDTLQSEETEVLTLTENTCRLIVWNDEVNTFEWVIET
                     LMEVCGHAHEQAEQCAYIIHFQGKYAVKNGPYDDLKPQCDAITERGINATIEVIAV"
     gene            2548..>2760
                     /locus_tag="PLO70_17060"
     CDS             2548..>2760
                     /locus_tag="PLO70_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQZ76232.1"
                     /translation="MAIFALDKELVFPPVHLSEPDGLLAMGGDLSPERLLLAYRHGIF
                     PWYEGEHILWWSPDPRFVLFPGELKVS"
BASE COUNT          870 a          530 c          600 g          760 t
ORIGIN      
        1 gtcgggagaa tcggcggttc gaaaataaaa ttgaaataat cgggagttta gctcagctgg
       61 ttcagagcat ctgccttaca agcagagggt cggcggttcg aacccgtcaa ctcccacacc
      121 taagcctctt tatcgaggct tttttgttta tgtggacagt ctacataatt tattccagga
      181 ttaaagatcg gtattataca ggttttacag gtgattctat tactgaaaga atcagaaaac
      241 ataacaccag gcactcgggg tttaccggtg gtggttctgg agattgggaa ctccaatatt
      301 ctgaaacttt tactaataaa gaggaggctt caaaaagaga gcgggaaata aaaagctgga
      361 agtccaggaa aaagattgaa aagcttattg gggaagggca ccccgactaa cagtcgggag
      421 ggtcggcggt tcgaacccgt caactcccac ttacaagacc tctaagatac tttgatttta
      481 agtgttttag aggtcttatg ctattagcag gttactacaa aatgaaccct aaaagcccca
      541 tttacttgta aattgcttct aagttttcgg ctgactatat gctcaatctt aaagagcatg
      601 gaaattacat tcaggttgat cattgaccag gtaaatcaaa aaattttgca ctgtgtctta
      661 gaatgggaat gtgcaattaa gccaaaagaa ttgatatgcc tgacaaacct gagcagggtc
      721 atcatatccg attgtcccta cagcgtacct tacatcagaa tcaaaaaaat taaattatga
      781 aaaagatttt cagtgctttg tttttgctag caggagcaat cacaatttta agcactgcag
      841 ccccaggctg taaagccaaa cccaaggatg ccaatattaa aacttcgatt gaagcagctt
      901 tgaagtccga tccgcagact tcaggcctgt cagttgatgt taaggaagga gtggctaccg
      961 ttaccgggga ggttaaagat gcttcagtac aggcaactgt ggcaactata gttggcggaa
     1021 taaaaggtgt taagtcagta ctgaataatg taacggttac accaccagtt gttattacac
     1081 ccgatgatcc tcttactatg gctgttaaag atgcgacaaa agattttact gggattacag
     1141 caaccgtaaa ggatggaata atctacgtta ccggggaatt aaaatcagcc gactggaaaa
     1201 aattgaaaat ggcattggac gggttgaaac ctaaaaaagt agatgctgct ggtttgaaaa
     1261 tatctaaata aacaagtaaa tctaattatt atgcaactaa aagataaata caaagattta
     1321 atggatgccg ctgttgcatc aggagttaac aacctgcaag tccgagaaca ggatggcgtt
     1381 ttgtacattg atggcgaggc accaagtgga gttgtaaaag atcagctttg ggaaacctat
     1441 aacaaactgg atcctgattt tctttccgga gatgttgtca tgaatgtaaa tgtatccccc
     1501 tctattcctg gtacaaaagt caaagtgact acagaaagat caaatctgaa tattcgcaaa
     1561 gggcctggca cggatcaacc aattgtcggt aaagctgccc accatgaaat tgtaacattg
     1621 gtaagccaat caaatgatca gtggtggctg atacggacaa acgatggcga ggaaggctat
     1681 gcctatgcgc aatatcttac gccagaacca aattgacggc tcctatattg caggaaaatg
     1741 atgtagattt ttttttcact taaataaaat tttatgccaa tagtaaaagt catagaagtt
     1801 attgcttctt cgcctgtcag ctttgatgac gccatcaaaa attgtcttgc cgaagtttct
     1861 aaaaccgttc acaatattga ttctatctat gtgaaagatt tcaaagtgca tgtaaaagaa
     1921 ggaaagatca cttcctacgg tacaatttgc aaggtatcct tccgggtaga ttcataatac
     1981 agggacagta agtcaaaaac aggttatcta cctgtcggat ttattgcatt gggttaccaa
     2041 aatggttttc aaccaagaag gcttcaggga gacgaatatc aaagtatccc ttaggccttt
     2101 ttttctgtgc agccttccaa gtagtgggtc tgcggttcaa acctgtcaat tcccacatca
     2161 aagccatgtt attgagactt ttttttattg caataataat ctgaaataaa tttttacttt
     2221 tacaccctga atgaaagcta cagcaacgaa tacggataca ttacaatcgg aagaaacgga
     2281 ggtgcttacc cttaccgaaa acacttgccg gctgattgta tggaatgatg aagtcaatac
     2341 tttcgagtgg gtaattgaaa ccctgatgga agtatgcgga catgcccatg aacaggctga
     2401 acaatgcgct tatatcatcc atttccaggg caaatatgca gtaaagaatg gcccttacga
     2461 tgacctgaag ccccaatgtg atgcaatcac agaaagaggg atcaatgcta ccattgaagt
     2521 catcgccgta taattcattt ctgatccatg gccattttcg cccttgataa agaacttgtt
     2581 tttcccccgg ttcatttatc agaacctgat ggattactgg caatgggtgg tgacctttct
     2641 ccggaaaggc tattgctggc ttaccgccat gggatctttc cctggtacga gggtgaacat
     2701 atactctggt ggagtcctga tccgagattt gtattatttc ccggcgaatt aaaagtgagt
//
LOCUS       DAORTI010000263         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_53332_length_2745_cov_6.817100, whole genome shotgun sequence.
ACCESSION   DAORTI010000263 DAORTI010000000
VERSION     DAORTI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53332_length_2745_cov_6.817100"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1284)
                     /locus_tag="PLO70_17065"
     CDS             complement(259..1284)
                     /locus_tag="PLO70_17065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQZ76233.1"
                     /translation="MKSLRLFFFFLLSLVAVTAVLSLIMPVKQKLERTITIKAPAAVV
                     FEQLAKLENFNTWAAWNQQDSTVKHTISGTDGTVGAVTTWTGSPEISGDGRMEIVSIE
                     PGKKIVHAITFIKPKKGKAGSFFTLNESNGVSSVIWNFEMDTPRPWNIFNLFYSMDEQ
                     MGKDFEKGLAALKTIVEKTTATATSQKIYEVMPMNFPATTYAVVRQQVKWSDITSFFA
                     QHFQILYNEAEKNNAVQGAPAGLYYVWDEKNQQADMAAAIPVSSGTSFENTIIRLTDI
                     TASKAVYVNYYGAYDQTEDAYKSIDKYLADNKLKQKFPVIEQYITDPATEKDTANWLT
                     KIIFLVE"
     gene            complement(1303..>2745)
                     /locus_tag="PLO70_17070"
     CDS             complement(1303..>2745)
                     /locus_tag="PLO70_17070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQZ76234.1"
                     /translation="DKEGVKIPSPAEVNNVLSAVDIAAIEPYKEKVLATSLIEPGKLK
                     PGKIISSKADEELGTTTIKLANGATVLLKPTNFKNDEILFRAFSKGGHSLVKDADYYS
                     AINAANIVVQSGVGNFSAIDLGNMLKGKNTSLTPTIGTYSEGMSGNTIPKETETLLQL
                     IHLYFTAPKKDAAAFESFKTRQKQLYANIAANPQIYFSGEFQKIMTQNHPRAGALPKP
                     TDFDKINLDRSIQIYKERFSNAGDFTFLFTGSFDEDSIKPLLEKYIGSLPAIAKKETY
                     RDLGIRPPKGKVDKVITKGEDPKSLVNLVFTVPAVYNTNEAYALLSLGELMNIKLVEQ
                     LREEKAGVYGVSAYGGMNRIPYSSSTFNISFPCAPENADTLTAAAIDELRKIIKTGVS
                     AEDLEKIKEQQKRKLEVDIKQNIFWSNNLFDAYFYGTNPSDILNKQKMIDNLSSKIIQ
                     DAAKKYINLNSYIRATLKPAKQEEKPLKAF"
BASE COUNT          711 a          563 c          538 g          933 t
ORIGIN      
        1 tgtaagcttc cacgatagca tcttccagtt ctttgggaat ggggaaaata gagggatcaa
       61 aatcaccgat cgtgaaatta taaatacgtt ctccctgacg gatcttttcc cgtatttctc
      121 cacccagttt tacaatttca gacccaatca gcgtttcaga aagatgcgac agtttcataa
      181 cccaaccttt gcggcaaaag taaatggatt ggtggagata gcaaaccgtt tgaccccggt
      241 aattacaaaa gtctttcttt attctaccag gaaaataatt tttgtcagcc agtttgcagt
      301 atctttttcg gtggcaggat cagtgatata ttgttcgata accgggaatt tttgttttag
      361 tttattgtca gcaaggtatt tatcaatgct tttataagcg tcttctgttt ggtcataagc
      421 cccataatag tttacatata ctgctttgga agcagtaata tcggttaatc gaataatggt
      481 attttcgaaa ctggtcccac tgctaacggg gattgctgct gccatatccg cctgttgatt
      541 tttttcatcc catacatagt acaatcctgc cggtgctccc tgtactgcat tatttttttc
      601 cgcttcatta taaaggattt gaaaatgctg cgcaaaaaaa gaggtgatat cactccattt
      661 tacctgctga cggacaactg cataggtagt ggcggggaaa ttcataggca tcacttcata
      721 aatcttttgg gaagttgcag tggcagttgt tttttcgaca attgttttca aagctgccaa
      781 tcctttttca aaatcttttc ccatttgctc atccatactg taaaaaagat tgaaaatatt
      841 ccagggcctg ggtgtatcca tttcaaaatt ccatatgacg gagcttacac cgttagattc
      901 attcaacgtg aaaaaagaac cggcttttcc ctttttgggt ttaataaagg taatagcatg
      961 tacaattttt ttacctggtt ctatagatac aatctccatc ctcccatctc ccgatatttc
     1021 agggcttcct gtccatgttg ttacggcgcc cactgttccg tcagttccgg aaatggtatg
     1081 ctttacggtc gaatcctgct gattccatgc ggcccatgta ttaaaatttt ccaatttggc
     1141 caattgctca aaaacaacag cagccggggc cttgatagta attgttcttt caagcttttg
     1201 ttttacaggc attataaggg aaagaacagc tgttacggct accagtgaca gtaggaaaaa
     1261 gaaaaatagg cgcaatgatt tcatggctgt tagttaataa tgctaaaatg cttttaatgg
     1321 cttttcttct tgtttagcag gtttcaacgt cgctcttatg taactattca ggttgatata
     1381 cttcttcgcc gcatcctgaa ttatttttga actgaggtta tcaatcattt tctgtttatt
     1441 cagaatatcc gaagggttgg tgccatagaa ataggcatca aacaagttat tagaccagaa
     1501 gatattctgc ttgatatcca cttccagttt tcttttctgc tgttctttga ttttctcaag
     1561 gtcctcggca cttactcctg ttttaattat ttttcttaac tcatcaatgg ctgcagctgt
     1621 taaagtatct gcattttcag gagcacaggg aaatgaaata ttgaaagttg aactgctata
     1681 aggaatcctg ttcatgccac catatgcact aacaccatac accccagctt tttcttcccg
     1741 aagttgttct accagtttta tattcatcag ttcccccagg ctcagaaggg cataagcttc
     1801 attggtattg tatactgcag gaactgtaaa taccaggttg accaggctct ttggatcttc
     1861 gcctttggtg ataaccttat ctactttacc ctttggagga cggataccca ggtcccggta
     1921 ggtttctttt ttcgctatag ccggtaagct tccgatatat ttttctaata atggcttgat
     1981 tgaatcctca tcaaaggagc cggtaaacag aaaagtaaaa tcaccggcat tggaaaatct
     2041 ttctttatag atctgaatac tgcggtcaag atttatttta tcaaaatcag ttggtttagg
     2101 caatgctccg gcacggggat ggttctgggt cattattttt tgaaattctc cgctaaaata
     2161 tatctgggga ttggccgcga tattcgcata cagttgtttc tgcctggttt taaaggattc
     2221 aaaggctgct gcatctttct tgggggcagt gaaataaaga tggatcagct gcagcagcgt
     2281 ttctgtttct ttaggaatag tatttccact cattccttcg ctgtacgtac caatggtagg
     2341 ggtcaggctg gtgtttttcc ctttcagcat attaccaaga tcgatagcag aaaagtttcc
     2401 caccccgctt tgcactacaa tgtttgcagc atttattgct gaatagtaat ctgcatcttt
     2461 taccagtgaa tggccgcctt tgctgaaagc cctgaataaa atttcatcgt tcttgaagtt
     2521 ggttggtttt aataatacag ttgcgccgtt agctaatttt attgtagtag ttcccagctc
     2581 ttcatcagct ttcgaactga tgatttttcc tggtttcagt ttgcccggtt ctatcaaact
     2641 ggtagcaagc actttttctt tgtagggttc aatagctgca atatctactg ccgacagcac
     2701 attgtttact tctgctgggg aaggaatttt gacaccttct ttatc
//
LOCUS       DAORTI010000264         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_53611_length_2736_cov_5.951138, whole genome shotgun sequence.
ACCESSION   DAORTI010000264 DAORTI010000000
VERSION     DAORTI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53611_length_2736_cov_5.951138"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLO70_17075"
     CDS             <1..684
                     /locus_tag="PLO70_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQZ76235.1"
                     /translation="VAGVTIIRNPITAARAVMEKSPHVMMTGAGAEKYAKQQGLEIVD
                     PSYFYTEERWRGLQKAKQQDSLKMYLDHADTTNRGMLKQWENKDYKYGTVGAVAFDRY
                     GNLAAATSTGGMTNKKFGRVGDAPIIGAGTYANNNTVAISGTGWGEYFIRLVMAKSIS
                     DMMEFGKMKLNVAADEMVMKKLPALGGDGGLIAVDKQGNIAMPFCTEGMYRGYIKNTG
                     EKVIKIYKE"
     gene            complement(701..2245)
                     /locus_tag="PLO70_17080"
     CDS             complement(701..2245)
                     /locus_tag="PLO70_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HQZ76236.1"
                     /translation="MKKIFFIIASFVSSLPLFAQEPADALRYSWYTSSGTARQQAVGG
                     AMASLGGDLSAAYVNPAGLGFYKTGDFVLTPAYNFLNNNATYYGRSEKDKRNYLSFGT
                     TGLVAGSGTDKDRRGRSSSSSFAIAINRTANFSSNLLYRGENNQSSYSNKFLEEINND
                     RDANSVAQNYPFGTSLAFNTYWIDTIAGGSSGNYQFQTRAPIGTGLLQENVITNRGGI
                     TELALAFAGAQRDKFYFGFTLGVPFLRYDKESTFTEADATSNTDNKFDYAAITENLTT
                     KGTGLNLKMGVIYKPVEYVRLGLAVHTPTFYRLTDRYNASITTNTESYNGIQTQSSDI
                     FTGGQDAEFAYWMFTPYRIMASASYVLREIEDVRKQKGFLTADIEYVNYKASSYTTDP
                     EGDNSQNTKDYLKSLNGAIDNAYKGAFNFRAGGELKFTTIMARLGMAYYGNPYKNLAG
                     EKGNRFQLTGGLGYRNKGMFIDLAYVHTMGKDVHFAYRLQNRPFSGAGLKQTGGNAIV
                     TLGFKI"
     gene            complement(2333..>2736)
                     /locus_tag="PLO70_17085"
     CDS             complement(2333..>2736)
                     /locus_tag="PLO70_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76237.1"
                     /translation="YNPYNSWNYYYNPYCHNTVFVNTKYVTTYNKPRTFNLNTYTNNT
                     TGTVKNTTYSNPKYGLPGSNNNSNNNSYNQPRNSTRTSSNSGNVLRDIFRGSNSSSSG
                     NSNPSSSSSSSSSSSSSSSGSSGGSAPVRRF"
BASE COUNT          781 a          522 c          622 g          811 t
ORIGIN      
        1 gtcgctggcg tcacaatcat tcgtaatcct attactgctg caagagcagt gatggagaaa
       61 agtccccatg tgatgatgac gggagcaggg gctgaaaaat atgctaaaca acaggggctt
      121 gaaattgtgg atccatccta tttctatacg gaagaaagat ggagaggttt gcaaaaagct
      181 aagcaacagg attctttaaa aatgtattta gatcatgcgg atacaaccaa tagagggatg
      241 cttaagcaat gggagaataa agattacaaa tacggtactg ttggtgctgt cgcatttgac
      301 cgttacggaa acctggctgc tgctacaagt accggcggaa tgacgaataa aaaattcgga
      361 agagtaggag atgccccgat aattggtgcg ggtacttatg caaacaacaa taccgttgct
      421 atcagtggaa cagggtgggg tgaatatttt atccggttag tgatggcaaa gagtatcagt
      481 gatatgatgg aatttggtaa aatgaaacta aatgttgctg cagatgaaat ggtgatgaaa
      541 aaattaccgg ctttaggggg cgatggtgga ttgatcgccg ttgataaaca gggtaatata
      601 gcgatgcctt tctgcaccga aggtatgtac cgtggttaca taaaaaatac cggggaaaaa
      661 gtaataaaga tctacaaaga ataagaacca gtttccggct ttaaattttg aaacctaaag
      721 tcacaatagc atttccacct gtttgtttta atccggcccc tgaaaatggc ctgttttgca
      781 aacgataggc aaaatgcaca tccttaccca ttgtatgtac ataggcaaga tcgatgaaca
      841 ttcctttgtt cctgtaccct aaaccacctg taagctggaa gcggtttcct ttttctcctg
      901 caagattctt ataagggttg ccatagtaag ccatacccag acgggccatg atggtggtaa
      961 attttaattc gcctccggca cggaagttaa atgccccttt gtatgcgttg tcaattgccc
     1021 cgttaagcga tttcagatag tctttggtgt tctgactatt atcgccttcg gggtctgttg
     1081 tataggagga tgctttatag ttcacatatt caatatcggc agttagaaat cctttttgct
     1141 ttcttacatc ttcgatttca cgtaaaacat aggaggcact ggccattatt cgataagggg
     1201 taaacatcca gtaagcgaat tccgcatcct gtcccccggt gaagatgtct gagctctgag
     1261 tctgtattcc attataactt tctgtattgg tagtaataga ggcgttgtac ctgtcagtca
     1321 ggcgataaaa ggttggtgta tgtacagcta agccaagcct tacatattcg acaggtttat
     1381 aaattacacc catttttaaa tttaagcctg taccctttgt tgtcaggttt tcagtaatgg
     1441 cagcatagtc aaatttgttg tccgtattgc tggttgcatc tgcttctgtg aaggtggatt
     1501 ccttatcata tctcaaaaaa ggtacaccca gtgtgaatcc aaaatagaat ttgtcacgct
     1561 gtgcaccagc aaaagccagc gccagttccg taatgccacc cctgttggta attacatttt
     1621 cctgtaacag accagttccg atcggtgctc ttgtctggaa ctgataattt ccggaagagc
     1681 ctccggctat tgtatctatc cagtatgtat taaatgccag gctggtacca aaaggatagt
     1741 tttgtgccac gctattggca tcacggtcgt tattaatctc ttccaggaat ttatttgaat
     1801 aggagctttg attattttct ccgcggtaca ggagattgct gctaaaattg gcggttctat
     1861 taatagctat cgcaaaagaa gaactgcttg atctccctct tctgtcttta tcagtaccac
     1921 ttcctgcaac caggccggta gtcccaaacg acaaataatt tcttttgtcc ttttcagaac
     1981 ggccataata agtagcatta ttgtttagaa aattataggc cggtgtcaat acaaaatcgc
     2041 ctgttttgta aaagccaagt ccggcaggat ttacataggc tgctgaaaga tcacctccca
     2101 gggaagccat cgctccgcca acggcttgtt gcctggcagt accggaagag gtgtaccagg
     2161 aataacgaag ggcatcagca ggttcctggg caaaaagggg caatgatgat acaaaagagg
     2221 ctatgatgaa aaatattttc ttcatgatat cttgaattat aggtagagaa aacttagttc
     2281 agaaagactg aacctgtttg gcctgaactt catactagtg tgttcgaaat aattaaaatc
     2341 ttcttactgg tgcacttcca ccactgctgc cagatgagga agagcttgaa ctgctggaag
     2401 aagaactgct gctgctcgga ttactgttac cggatgaact actattacta cccctgaaaa
     2461 tatctcttaa cacattccct gaattgctgc tggtacgggt actgttccgg ggttgattat
     2521 aggagttgtt atttgaattg ttgttactgc ccggcaaacc atactttgga ttgctgtagg
     2581 tggtattttt aaccgtaccg gttgtattgt tagtataagt attcaggtta aatgttcttg
     2641 gtttattgta ggtggtaacg tatttcgtat tgacaaaaac agtgttgtga caatagggat
     2701 tataataata gttccatgaa ttatagggat tgtaat
//
LOCUS       DAORTI010000265         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_53849_length_2729_cov_4.201571, whole genome shotgun sequence.
ACCESSION   DAORTI010000265 DAORTI010000000
VERSION     DAORTI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53849_length_2729_cov_4.201571"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1095)
                     /locus_tag="PLO70_17090"
                     /pseudo
     CDS             complement(161..1095)
                     /locus_tag="PLO70_17090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
     gene            1225..>2729
                     /gene="katG"
                     /locus_tag="PLO70_17095"
     CDS             1225..>2729
                     /gene="katG"
                     /locus_tag="PLO70_17095"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQZ76238.1"
                     /translation="MENNTAQGKCPFTGGALKQSAGGGTRNRDWWPNQLKLNILRQNS
                     SLSNPMGETFNYAEEFKSLDLAAVKKDIFDLMTTSQDWWPADFGHYGPFFIRMAWHSA
                     GTYRIQDGRGGAGNGTQRFAPLNSWPDNVNLDKARLLLWPIKKKYGKKISWADLMILT
                     GNCALESMGLKTFGFAGGREDVWEPEEDIYWGSEKEWLDDKRYSGDRELENPLAAVQM
                     GLIYVNPEGPNGNPDPLAAARDIRETFGRMAMNDYETVALIAGGHTFGKTHGAADPSK
                     YVGPVPAAAGIEEQGLGWKNTYGTGNAGDTISSGLEVTWTTTPTKWSNNFFWNLFGYE
                     WELTKSPAGAHQWQPKHGMGAGTVPDAHDPSKRHAPMMLTTDLSLRFDPVYEKISRHF
                     LENPEEFADAFAKAWFKLTHRDMGPRSRYLGPEVPEEELIWQDPVPAVTHKLVDENDI
                     VVLKNKVLTSGLTVSQLVSTAWASASTFRGSDKRGGANGARIRLAPQKNWEA"
BASE COUNT          745 a          583 c          636 g          765 t
ORIGIN      
        1 gacatgcaaa ataggtaagt tttgtgaaag cagggttgta atattcctgc tattaccggt
       61 acggtcaaca acgggtagta tccaggctat atctgaagta tttttcattg tggtgtattc
      121 cggttgttaa acggtatcaa tacgtcggat gcccaactgg tcaataattt atttctacta
      181 tttttttatg ctgcaatttt tgcatttgca cgggtacaat atttacaatt tcttcccgaa
      241 gcgtttttat aagcttggtt ttaatatgat cacgatgggt aaccaggctt acttcccggg
      301 cgggacttgg ctgttttaat cgctttacga gtttaagcct ggctttgctg aactccatta
      361 cggccaactc aggcagaatg gtaatgccgt tgcttttatc caccattctt ttaagggttt
      421 caatattacc tgtttcatat ttaaaatgaa agtcactgct tttttttaac tcgcaaatat
      481 ttaatacctg cgaccggaag caatgccctt cttccagcag ccaaagttca ttgggattaa
      541 tttcactggc cagcacatac tttttgccag acaatgcatt ctttttagag acataaacaa
      601 aaagttcttc ataaaaaagt atatcttctt ttatggatgg gtcttttaag ggagtaacaa
      661 caatgccgca gtccaaccgg ttgttttgag ttcatagatc acatcttctg taatggactc
      721 cttaatgata agatttaact gagggtattt atcctgcata gatttataaa gtgagggcaa
      781 taaatagggc gccagtgtag gaatgatacc catgcgtaaa tcccctgaaa gggtatcctt
      841 ctggtcgttg atcatctgtt tgatcaggtg ggcttcccgc agggttatgc gtgcctgcct
      901 tataatgttt gtgccgatct ctgtagggat gaccggctgt ttggtacggt caaaaatttt
      961 tacgcccagt tcctcttcca gtttttgaat ctgcatactg agggtgggtt gcgtgataaa
     1021 acatttttct gcagccaatg caaaatgccg gtgggtatcc aatgcaacaa tatattcaag
     1081 ttgggttagt gtcatagaca gtgactataa gatcataaaa ataatcaatt tgatttattg
     1141 ttgacgctgc tgtagttttg aaatcttagc aaattgcaca cgattaaaca atcattacaa
     1201 gtaaattaat tcaatcttca gagtatggaa aataacacag cacagggcaa atgcccgttt
     1261 acaggaggag cgctgaaaca aagcgcaggc ggtggtacta gaaaccgtga ctggtggccc
     1321 aaccagttaa aactaaatat acttcgtcaa aattcttcac tgtctaaccc gatgggagag
     1381 acatttaatt atgcggagga attcaaaagt ctggacctgg ctgccgtaaa aaaagacatt
     1441 tttgatctga tgactacctc ccaggactgg tggccggctg attttggcca ttacggtcct
     1501 ttctttattc gaatggcctg gcatagtgca gggacatacc gtatccagga cggtcgtggt
     1561 ggtgcgggca acggtacaca aaggtttgca ccgctcaaca gctggcccga taatgtgaac
     1621 cttgataagg cccgattact gctgtggccc attaaaaaga aatatggtaa aaagatatcc
     1681 tgggccgacc tgatgatcct caccggcaac tgtgccctcg aatctatggg attaaagaca
     1741 ttcggattcg ccggtggccg tgaggatgtg tgggaaccag aagaagatat ttattggggt
     1801 tccgaaaaag aatggcttga tgacaaacgt tattccggcg accgggagct cgagaatcct
     1861 ttggccgcag ttcagatggg gcttatctat gttaaccccg aaggtcctaa tggaaatccc
     1921 gatccactag ctgcagcccg tgatatccgt gaaacttttg gtcgtatggc gatgaatgat
     1981 tacgaaacgg tagcattgat tgcagggggg catacttttg gtaaaactca cggtgcggct
     2041 gacccttcca aatacgtagg ccccgtacct gcggcagcag gtattgaaga acaagggctc
     2101 ggctggaaaa acacctacgg cactggtaat gccggcgata ctattagcag cggactggaa
     2161 gttacatgga ccaccacacc tacaaaatgg agcaacaact ttttctggaa cctgtttggt
     2221 tacgaatggg aacttaccaa gagcccggcc ggtgcacatc aatggcagcc taagcatggt
     2281 atgggagccg gtacagtgcc ggatgcacat gatccatcaa aacgtcatgc acccatgatg
     2341 cttactactg atctttccct gcgttttgat ccggtctatg aaaagatctc acgccatttc
     2401 ttagaaaacc ccgaagaatt tgcagatgct tttgccaaag catggttcaa gctcactcac
     2461 cgtgatatgg gaccccgttc ccgttacctc ggaccagaag tgccggaaga agaactgatc
     2521 tggcaagatc ctgttcctgc agttacacac aaactggttg acgaaaatga tattgttgta
     2581 ttaaagaata aggttcttac atcagggctc accgtatcac aactggtatc cactgcatgg
     2641 gcttctgctt ccacattccg cggatcagat aagcggggtg gagccaatgg tgcccgtatt
     2701 cgtttggctc cgcaaaaaaa ctgggaagc
//
LOCUS       DAORTI010000266         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_56145_length_2661_cov_4.488872, whole genome shotgun sequence.
ACCESSION   DAORTI010000266 DAORTI010000000
VERSION     DAORTI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56145_length_2661_cov_4.488872"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLO70_17100"
     CDS             complement(<1..230)
                     /locus_tag="PLO70_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76239.1"
                     /translation="MTEKKEYITQIENLSAEQIADGISIGIVTFDELRRTGAFDASKQ
                     KSVKNILKKKDDDTFASAQTIRDLQNYLRVFP"
     gene            complement(251..1195)
                     /locus_tag="PLO70_17105"
     CDS             complement(251..1195)
                     /locus_tag="PLO70_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76240.1"
                     /translation="MKQSKFSLADVLTLLTALAFGFICFLGKNFSTLGNTSESITWAA
                     IITVSLAGTAFIAKLLKRTSRNFKTSFILEIGVLLLFTGLLVFFAYSPFPHYFNVSAK
                     KTKIQDKLKISITKAENMFADYEQYAENRKLNYQGQLETAIATKLTGGDYVKYGFSDG
                     IASDATQMRNKMFTIHADLYPTNYSDTASKNGIKEVATAWLARAKKTTKSWKPGIVNV
                     VNDIESKSTDWLNQLISFSQRLEQGESRENTTVFPFPLTFTDVKKYFTTPGKPTPLSI
                     GLAAVAYLLMLLSYLISKRSSKTMIGTTKQKGEYDIDF"
     gene            complement(1253..>2661)
                     /locus_tag="PLO70_17110"
     CDS             complement(1253..>2661)
                     /locus_tag="PLO70_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQZ76241.1"
                     /translation="KAPQAWAITRGSEKITVAIVDDGFNLKHPELASKVVSPYNVWKH
                     SAEVFPQPIDHGTHVAGIALAIANNGKGISGIAPNCKFMPVQVANEKGLMTTTSVLDG
                     IIYSLYQGADVINVSLGGQFTGLSQYTDNVQKDLIRNHFKEEERLWRRVMRIAASHNA
                     TLVVAAGNDNVLAGIDALQRPELFITVSATDKNNQNARKAQFSNYGSYSTISAPGVGI
                     YSTVGKDSYATMDGTSMAAPIISGTVALMKSINDTITTKQIICILKSTGLETQGNIGK
                     LIQLDKALQKVRSGEVVDCTPVPSSGDVQILLSWNNYNDLDLICTDPNGESVFFRNRN
                     VSSGGQLEIDMNVEYPDSKTPIENIFWQPGTAPNGTYNVYLLYYKNHDPNLNETPYNI
                     KVKYSGKTEEFKGVIRKKDKPVHICSFTVGATGNRSKNQGSPPANDRQSQLEQERNRL
                     QQELDRINNEIKRIGNSR"
BASE COUNT          712 a          537 c          503 g          909 t
ORIGIN      
        1 ttaggaaaaa ctcgcaaata attttgtaaa tcacgaatag tttgagctga agcaaaagta
       61 tcatcatcct tcttttttaa aatgtttttt acactttttt gctttgatgc atcaaaagca
      121 ccagttcttc gtaattcatc aaatgttaca attccgatgg agataccatc ggcaatttgt
      181 tctgcactta agttttctat ttgagtaata tattcttttt tttcagtcat cattatggtg
      241 gttaattatg ttagaaatca atatcgtatt ctcctttttg tttggtggtg ccaatcatcg
      301 ttttactgct tcttttcgaa attaagtacg acaacaacat caacaaatat gctactgcag
      361 ccaaaccaat tgaaagcggc gttggcttgc ccggggtggt aaaatatttt tttacatctg
      421 taaaagttag agggaaagga aaaaccgtag tgttttctct gctttctcct tgttcaagtc
      481 tttgggaaaa gctaatcagc tgatttaacc agtctgttga tttgctttct atatcattta
      541 ccacatttac aatgcccggt ttccagctct ttgtggtttt ctttgctctt gccagccagg
      601 cagtagcaac ttcttttatg ccatttttac tagcggtgtc actatagttt gtaggataga
      661 gatcagcatg gatggtaaac attttatttc tcatttgagt tgcatctgaa gcgataccat
      721 ctgaaaatcc atatttaacg taatctccgc ctgtaagctt tgtggctatc gctgtttcta
      781 actgtccttg gtaattaagc ttacgatttt cggcatattg ttcataatct gcaaacatat
      841 tttcagcctt cgtaatgctt attttaagtt tgtcctggat ttttgtcttt ttagccgata
      901 cattaaaata atgcgggaat ggcgaatacg caaagaaaac caagagacca gtaaacagca
      961 gcagtacacc tatttccaaa ataaaactgg ttttgaaatt gcggcttgtt cgttttagta
     1021 atttggcaat aaaagctgtg ccagccaatg atacggtaat aattgcggcc caggtaatac
     1081 tttcggatgt gttacccaga gtagaaaagt ttttaccaag aaaacatata aagccaaatg
     1141 caagagctgt taataaagtt agcacatcag ctaatgagaa ttttgattgt ttcatatttt
     1201 accttcagtt attaaacctt tgtttttaat tatttatcca attggccagt aatcacctgc
     1261 tatttccaat tctctttatt tcattattta ttctatctaa ctcttgttgc aaacggttac
     1321 gttcctgctc caactgcgat tgcctgtcat tagcaggggg tgaaccctgg ttcttagacc
     1381 tgttccccgt tgctcccact gtaaaggagc atatatgtac aggcttgtct tttttcctta
     1441 tcacaccctt gaattcttcc gtttttccgc tgtattttac ctttatgttg taaggtgttt
     1501 cattgaggtt aggatcgtgg tttttatagt acagtaaata cacattgtaa gtcccgttgg
     1561 gtgcagtgcc gggttgccaa aaaatatttt cgataggcgt tttgctgtcg ggatattcca
     1621 cattcatgtc aatttcgagt tgcccgccac tggatacatt tcggtttcgg aaaaaaacgg
     1681 attcgccatt ggggtcggta caaatcagat ccagatcgtt atagttattc caactaagca
     1741 gtatctgtac atcgccggaa gaaggaaccg gtgtgcaatc aacaacttca cccgaacgta
     1801 ctttttgtaa tgctttgtcc aattggatta gcttaccaat attgccttgg gtttccagac
     1861 ccgtactttt cagaatacaa ataatttgtt tggtagtgat ggtatcattt atgcttttca
     1921 tcagggcaac tgtaccggaa ataataggag ccgccatact tgtgccatcc attgtggcat
     1981 agctatcttt acctaccgta ctgtatatac ctactcccgg tgccgaaata gttgagtaag
     2041 aaccataatt actaaattgg gctttgcgtg cattctgatt atttttatct gttgctgaaa
     2101 cggtaataaa caactcgggg cgttggagag catcaatacc tgccagtaca ttatcattac
     2161 ccgctgctac cacgagtgtt gcattatggc tggcagcaat tcgcataacc ctgcgccata
     2221 aacgttcttc ttccttaaaa tgatttcgga tcaggtcttt ttgtacatta tcggtgtatt
     2281 gtgaaagccc tgtaaattgg cctcctaatg aaacatttat cacatccgca ccctggtata
     2341 aggaataaat aatgccatcc aatactgaag tagtagtcat tagtcccttc tcgttggcta
     2401 cctgaacagg cataaacttg caattagggg caatacctga aatgcctttt ccattgttgg
     2461 caatagccaa cgctattcct gctacatgag tgccatgatc aatcggttgc gggaaaacct
     2521 ctgcagaatg tttccataca ttatacgggc taactacttt gcttgccagt tcaggatgtt
     2581 tgaggttaaa gccatcgtca acaatggcta ctgtaatttt ttcggaacct cgtgtaatag
     2641 cccatgcctg cggggccttt a
//
LOCUS       DAORTI010000267         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_56658_length_2646_cov_5.484755, whole genome shotgun sequence.
ACCESSION   DAORTI010000267 DAORTI010000000
VERSION     DAORTI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56658_length_2646_cov_5.484755"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLO70_17115"
     CDS             complement(<1..445)
                     /locus_tag="PLO70_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76242.1"
                     /translation="MRIIIFCFVFLLQCAMLSAQTVGIGTTTPNASAQLDIVSTNKGL
                     LIPRMTAAQRAAIASPAIGLMVYETTTNSLWLYNGTVWVQQSSGGVSPWAVSGNNIYN
                     TNSSNVGIGTSTPSSKFSVAGNLLVTGGDISINDPYGTLFFQVSST"
     gene            complement(479..1582)
                     /locus_tag="PLO70_17120"
     CDS             complement(479..1582)
                     /locus_tag="PLO70_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76243.1"
                     /translation="MKKIIICLLAIIQYQLLPAQGVGIGTTNPNSSALLDITSTTKGL
                     LMPRMTTAQRNAIVSPAEGLLIYNTTTEELNQRQNGAWKIVINSDYWFRGAGTMWNIG
                     DNIGINTAGPGERLDVSGNIRTNSSLIINNATSVLQLQNAGVNKGFVQLSGDNLRLGT
                     NSGNTNGNVVLRMDGTDMIEFNKTLSAGTSVQLNLNGVSTGVLQTTSTGNVSLTAVNA
                     NTQVQLGGEVFINNTANRTGIGTSSPAERLHVNGNIIVNGNAVIDDGRITGTATSSAY
                     NLLPLAYGRVTYLGNKAGGTPNFTSATRTGPGAYDVFVTGLTSSSVIMVTPGSISSIE
                     MNYDSPGKFRVRVWSSIYDEFNDCDFHFVIYNP"
     gene            complement(1595..1903)
                     /locus_tag="PLO70_17125"
     CDS             complement(1595..1903)
                     /locus_tag="PLO70_17125"
                     /inference="COORDINATES: protein motif:HMM:NF016197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HQZ76244.1"
                     /translation="MTETILIAEKINEVREEMRKTGTWKNRLPEWVKEFGKRRTDAGD
                     DFSEWLQFIYLPNMLLEVENKQQLPEKIFIVPQAVQFLGSDIQKGKLLQLLIELDSIA
                     "
     gene            2081..>2646
                     /locus_tag="PLO70_17130"
     CDS             2081..>2646
                     /locus_tag="PLO70_17130"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQZ76245.1"
                     /translation="MILNRYILIVWLSVVLPATGFTQSSQYRLETFGTRDGLLSSKVY
                     ALAQSSDRMLWIGTELGVSVYDGYSFTNYQYTSGNESIGRILCLTQDKLNGMWLGGDK
                     GLFYFKGDSIIKVELQNKTLLAVETLLTDATGNVWAGDLNALYKITATQVENLNKNNL
                     STLPLSPFASFNERVFSLATDNRQNIYWG"
BASE COUNT          739 a          575 c          531 g          801 t
ORIGIN      
        1 cagtactgga aacctggaaa aaaagtgtac cataaggatc attaatactt atatcgccgc
       61 ccgtaaccaa tagatttccc gccacactaa attttgatga aggtgtactg gttcctatac
      121 ccacattact gctgttggtg ttataaatat tattacccga taccgcccag ggagaaacac
      181 cacctgaact ttgctgtacc caaaccgtac cattatatag ccagagacta ttagtggtcg
      241 tttcatatac cattaatcct attgcaggag atgcaatggc tgcccgctgc gcagccgtca
      301 tccggggtat cagtaacccc ttgttggtac tgactatatc caattgtgcg gatgcattag
      361 gagtagtagt tccaatgcct acagtttgtg cagagagcat tgcacattgc agtaaaaata
      421 caaagcagaa aattattatt ctcataaccg aattttaatg atgaacagaa attgagtatt
      481 atggattgta gataacaaaa tgaaagtcgc agtcgttaaa ttcatcgtat atgctactcc
      541 atactcttac tctaaactta ccgggagaat cgtaattcat ttcaatgctg ctgatggatc
      601 ctggtgttac cattattacc gaagagctcg ttaatccggt aacaaaaaca tcataggcgc
      661 ccgggccggt acgtgttgca gaagtaaaat ttggggtacc tccagccttg ttaccaagat
      721 aggttaccct tccataggca agtggcagca gattataagc tgagctggtg gcggttcctg
      781 ttattcttcc atcatcaatc accgcatttc cattcacaat tatatttccg tttacatgca
      841 atctttctgc aggtgaggaa gtaccgattc ctgtgcggtt ggccgtatta ttaataaaaa
      901 cttcaccccc caactgcacc tgtgtatttg cattaaccgc tgtaagagat acgttaccgg
      961 tactggtggt ttgcaataca cctgttgaaa ccccattcag gtttagttgc actgaggttc
     1021 ctgcactaag agttttgttg aattcgatca tatccgttcc gtccatcctt aacactacat
     1081 tcccgttggt attaccgcta ttggtaccca gccgcaggtt atcgcctgat aactgaacaa
     1141 agcccttatt taccccggca ttttgcaatt gaagtacaga agttgcatta ttaataataa
     1201 gagaactatt agttcgaata tttccgctga cgtccaggcg ttcacccgga cctgcagtat
     1261 tgatacctat attatccccg atattccaca tcgtgccggc acccctgaac caataatcgc
     1321 tgttaatcac tattttccac gcaccgtttt gccgttggtt aagttcttca gtagttgtat
     1381 tgtaaataag caatccttct gccggggaca cgatggcatt acgttgtgcg gttgtcattc
     1441 ttggcatcag caaacctttt gtagtacttg taatatccag taatgcactg ctgttgggat
     1501 tcgtcgtacc gatgcccaca ccctgtgctg gcaataactg gtattgaatg attgccagta
     1561 aacagataat tatttttttc atgtacttta ttttttatgc tatcgagtcc agttcaatta
     1621 atagctgcag cagcttccct ttttgaatat cacttcccaa aaattgcaca gcctgcggta
     1681 cgataaatat tttttcaggg agctgctgtt tgttttcaac ttccagcagc atattgggca
     1741 ggtaaataaa ttgcaaccac tcactgaaat catcaccggc atccgtcctc cttttaccaa
     1801 actctttgac ccattccggt aacctgtttt tccacgtacc tgttttccgc atctcttccc
     1861 gcacttcatt tattttttct gcaatcaata tggtttcagt catgtggtgg ttttcaggag
     1921 gtaaatctat ttggggaaac ttgtctgtcc atcacctaaa aagccagcgg ggcaaaacat
     1981 ttatcgaatg gtacaaccag gcagtataat taggaaacgg tagttataat gagtgaatgg
     2041 caaaacaatc gggaaaagga tgtatatttt tactttataa atgatactca accggtacat
     2101 attgatcgtt tggctttctg ttgtattgcc ggctaccggg tttacacaat catcgcaata
     2161 caggttagaa acatttggca cccgtgacgg actcttgtca tccaaagtat atgcactggc
     2221 acaaagcagc gacaggatgc tctggatcgg tacagaatta ggggtaagtg tgtatgacgg
     2281 gtatagtttt accaattacc agtatacatc agggaatgaa tctatcggcc gcatcctttg
     2341 cctcacccag gataaattaa atggtatgtg gctgggtggc gataagggac ttttttattt
     2401 caaaggagat agtataataa aggtagagtt gcaaaataaa accttattgg cagtggaaac
     2461 cttacttact gatgccaccg gtaatgtgtg ggcaggggat cttaatgcgt tgtataaaat
     2521 aactgcaaca caggttgaaa atttgaataa aaataattta agtactcttc cactctcacc
     2581 ttttgcttcg ttcaacgaaa gagttttcag cctggctaca gacaacaggc aaaatatata
     2641 ttgggg
//
LOCUS       DAORTI010000268         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_57564_length_2622_cov_5.599143, whole genome shotgun sequence.
ACCESSION   DAORTI010000268 DAORTI010000000
VERSION     DAORTI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57564_length_2622_cov_5.599143"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1035
                     /locus_tag="PLO70_17135"
     CDS             88..1035
                     /locus_tag="PLO70_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76246.1"
                     /translation="MNRQDKIKLITAVLILVTQFSFAQKNGWKIAYVLWQDTTTVENS
                     IHSMDPDGENDKLIIDYPGSNWMPMTARYKIWFHLGKDTIGKKAGLYFYNLKNKTEKY
                     LFDAKGLYQDIDYHKGLKIYAGGFLHKPEGKTKSQYDIFLFNEDGSSKKPVTSDTAID
                     LEPVFTPDGKKIIFRSNRDRNPKSWAEFEIYSINTDGTGLKRLTYNPDTTQNVLRASS
                     PVITPDGRIVFAGYWEGTYRLMLMNMDGSDIKVLIKMDEMEQTSCSFSPDGKQVVFTG
                     RIKGKRNNDIYIVNTDGTNLRQLTNDWRRKVQPFIITSK"
     gene            1032..>2622
                     /locus_tag="PLO70_17140"
     CDS             1032..>2622
                     /locus_tag="PLO70_17140"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HQZ76247.1"
                     /translation="MKSAMSGKYFVPGFSTTIGFRCIALLCFLFAIQQHCYAQKGKGT
                     DRLYYTQLKTAEAYYRHNQIGEAKNILLAVPANKRTFEWQFLNARLDRSIKTLSSHTK
                     AVVGIAVSKDGKYFASGSADNSIIIWDAASCQLIRKIEAHKGQVTTLDFSPDSKTLIS
                     GSTDKTLRLWNIADGTEIRNFNADFKQGIYQCKFSSDGKMLGVVSWERGANGVQGFAK
                     VLDVQNGILIKRFDTDDHPASAVQFSADNKKLFTGTWGFQIKQHDIASVSTDWNYDMR
                     EFDYYTAVQSIDLSRDNKYVVQGGKDNKIRLLNAADGKLVYVIESWQGHKEWVNAVRF
                     SPDGKQFASISDDGLLKIWETATGINLFTFKGHIAGLNQLAWHPDGKRIYTTSADNTI
                     KVWDINAPGEMSFRASVIGPWNAPVSPDGNLLAPVNSDKYLALYSITTGKPLIFLDSL
                     SAFAATFSSDGQYLATGYRNVIVYNTATGKRLITGKGHTGVIYGMDYNAKLNLFVTAA
                     DNTIQLWKSTDTTAIKTISDIG"
BASE COUNT          843 a          457 c          600 g          722 t
ORIGIN      
        1 aatggaatgc catgtgtaca caccgcattc gtgtagaaga ggaaaagaca atcagtaaag
       61 atttgtttaa ttggataagg caggcgtatg aacaggcagg ataagataaa attaataact
      121 gctgttttaa tactggttac tcagttttct tttgcacaga aaaatggatg gaagattgca
      181 tatgtgttgt ggcaggatac tactacagtg gagaattcta ttcatagtat ggacccggat
      241 ggggaaaatg ataaattaat catagattat cctggtagca attggatgcc gatgactgcc
      301 agatataaaa tttggtttca cctgggaaaa gatacaatag gcaaaaaggc aggtttatat
      361 ttttataatt taaaaaataa aacggaaaaa tatctttttg atgcgaaagg tctttaccag
      421 gatattgatt atcataaggg gctaaaaata tatgcgggtg gcttcttgca taagcctgag
      481 ggaaaaacaa aatcacaata tgatattttt ttattcaatg aagatggctc ttcaaagaaa
      541 ccggtgacaa gtgatactgc aattgatttg gaaccggtat tcactccgga tgggaaaaaa
      601 attatttttc gcagcaaccg tgaccgaaat ccgaaaagct gggcagagtt tgagatttat
      661 agtatcaaca cagatggtac cgggctgaaa agactaactt ataatcccga tactacacaa
      721 aacgtattgc gggccagcag tccagttatt actcctgatg gacgcattgt tttcgccgga
      781 tattgggaag gaacctaccg cctgatgctg atgaatatgg atggcagcga tatcaaagta
      841 ctgattaaaa tggatgaaat ggagcaaact tcctgcagct tttcacccga tgggaagcag
      901 gttgttttta ccggcagaat caaaggcaaa cgaaataatg atatttacat cgttaataca
      961 gatggaacca acctccgcca acttaccaac gactggcgaa gaaaagtgca gccctttatt
     1021 attacatcga aatgaaatca gcaatgagtg gaaaatattt tgttcccggc tttagtacta
     1081 ctatcggctt ccgttgcatc gcactcttgt gcttcctgtt cgctattcag cagcactgct
     1141 atgctcaaaa aggtaaagga acagatagat tatactatac ccaattaaaa acagccgaag
     1201 cctactaccg gcataaccag ataggggaag caaaaaatat attactcgca gttcccgcaa
     1261 acaaaagaac ttttgagtgg cagtttctga atgcccggct ggataggagt ataaagactt
     1321 tatcttccca tactaaagca gtagtgggca ttgctgtaag taaagatgga aaatattttg
     1381 caagcggttc agctgataac agcattatta tctgggatgc agcatcctgt cagctcattc
     1441 gcaaaattga agcccataaa gggcaggtaa ccaccctaga tttttctcct gatagcaaaa
     1501 cactgattag tgggtctaca gataaaacac tccggctctg gaatatagcc gatggaacag
     1561 agatcaggaa tttcaatgca gattttaaac agggtattta tcagtgtaaa ttcagtagcg
     1621 atggtaaaat gctgggagtt gtttcttggg agagaggagc aaatggtgtg cagggatttg
     1681 ccaaagtact ggatgtacag aatggtattt tgataaaaag gtttgatacg gatgatcatc
     1741 ccgcttcagc agttcaattt tctgcagata ataagaaact ttttaccggt acctggggct
     1801 ttcagataaa gcagcatgat attgcatcgg taagcacgga ctggaattat gatatgcggg
     1861 aatttgatta ttataccgct gtgcaatcaa tagatctgag ccgggataat aagtatgtag
     1921 tacagggagg gaaggataat aaaatccgtt tgctgaatgc agcagatgga aaactggttt
     1981 atgtgattga gtcatggcag gggcataaag agtgggtgaa tgctgtccgt ttttctcctg
     2041 acggaaagca gtttgccagc ataagcgatg atggcttgct gaagatatgg gagactgcga
     2101 ctggtattaa cctcttcaca ttcaaaggac atattgcagg tcttaatcaa ttagcctggc
     2161 acccggatgg gaaaagaatt tatactacct ctgcagacaa taccattaaa gtctgggata
     2221 taaatgctcc cggcgaaatg agtttcaggg caagtgtgat aggtccctgg aatgcacctg
     2281 tttctcctga tggtaatttg ctggctcctg ttaacagtga taaatatctt gcattgtaca
     2341 gcataacaac ggggaaaccg ttgattttct tagatagcct ttctgctttt gctgccacat
     2401 tttcttctga tggtcaatat ttggcaacgg gatacagaaa tgttattgtt tacaatactg
     2461 ccaccgggaa aaggttaata accggcaaag ggcacacagg ggttatttac ggaatggatt
     2521 acaatgcaaa acttaattta tttgtaacag cggcggataa taccatacaa ctatggaaga
     2581 gtactgatac aactgctatt aaaactattt ctgatattgg ca
//
LOCUS       DAORTI010000269         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_58568_length_2595_cov_6.414961, whole genome shotgun sequence.
ACCESSION   DAORTI010000269 DAORTI010000000
VERSION     DAORTI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58568_length_2595_cov_6.414961"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..602)
                     /locus_tag="PLO70_17145"
     CDS             complement(24..602)
                     /locus_tag="PLO70_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQZ76248.1"
                     /translation="MEKIKSIIEASVKTKQQILQNNELIATIEKVVEVVTNAFKNGNR
                     VYFCGNGGSAADAQHLAAEFSGRFYTDRKALPAEALHCNTSYLTAVANDYGYDVVYSR
                     MIDGIGETGDVLIGLSTSGNSGNIIKAFEVAKDKGIITVAFTGITGGQMKAISDYLIN
                     VPSADTPRIQESHITIGHIICQLVEEKYFAPL"
     gene            complement(638..1660)
                     /locus_tag="PLO70_17150"
     CDS             complement(638..1660)
                     /locus_tag="PLO70_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HQZ76249.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPYSDLYGGAILNATLSLYANATI
                     EPTAANKIILEAIDRNEIQEFDWTKELPVTGTLDLLKGVYNRIQKDYGIKEQGFRLST
                     YVDAPAGSGLGTSSTLVVAIIGAFAEMLRLPLGEYDMAHYAYEIERRDLNLAGGRQDQ
                     YAATFGGVNFMEFYADDKVIVNPLRVKQQHLFELENNLVLYYTSTSRESAEIIRKQSK
                     NVTDNKEKSIEAMHHLKEQARMMKEALLKGRLNEIGEILDYGFQQKRQMAEGISNPLM
                     EEIYETAKKAGATGGKISGAGGGGFMIFYCPINTKYKVIKSLENFGGQVRNYQFTEHG
                     LKTWTI"
     gene            complement(1644..>2595)
                     /locus_tag="PLO70_17155"
     CDS             complement(1644..>2595)
                     /locus_tag="PLO70_17155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ76250.1"
                     /translation="GIIIHSAINSVNPFNWIRVGNKLRKERPDMIIVRFWLPFMGPAL
                     GTILRRVRKNRHTKIICIADNVIPHEKRFGDKPFTKYFLKSCDAFITMSEKVMSDLRI
                     FEKTKPAQLVSHPLYDNFGAIISKADARKQLHLPDNEKIILFFGFIRKYKGLDLLFEA
                     MANVRIKNSGIRLLVAGEFYEDAKQYQEQIERLGIKDRLILKTDFIPDSEVKYYLCAA
                     DAVIQPYRNATQSGVTPLAYHFEKPMVVTNVGGLPSLVPDKKVGLVVEPNPAAIAEGI
                     LNFYQLGENYFIPHLRNEKQKYSWATMAGTISQLANDIQK"
BASE COUNT          666 a          599 c          481 g          849 t
ORIGIN      
        1 taagtctctc ttttagggaa gatttagagg ggtgcaaagt atttttcttc gaccagctga
       61 caaatgatat gcccgatagt aatatgactt tcctgaattc ttggtgtatc agcggatggg
      121 acatttatca gataatcgct gatggctttc atctgtccgc cggtaatacc ggtgaatgcg
      181 acagttatga tccctttatc ctttgctact tcaaatgctt tgatgatgtt accggaattc
      241 cctgacgtag aaagcccgat caatacatca cccgtttcac caataccgtc tatcattcgt
      301 gaatatacca catcataccc atagtcatta gcaacagccg ttaaatacga agtgttacaa
      361 tgcaacgctt cagcaggcaa cgcttttctg tcagtataaa aacgccccga aaattctgcg
      421 gcgaggtgct gggcatcagc agcacttccc ccgttaccgc agaaatacac ccggttccca
      481 tttttgaatg cattggtaac tacctcaact actttttcaa tcgttgcaat gagctcatta
      541 ttctgcagta tttgctgctt tgtttttact gacgcttcga ttatgctttt aatcttttcc
      601 ataattccta attaacgaat ttaccaatac cggccagcta tatagtccaa gtcttcaatc
      661 catgttctgt aaactggtag ttcctgacct gccccccaaa gttctccaaa cttttaatca
      721 ctttgtattt tgtattgatg gggcaataaa agatcatgaa tccaccaccg ccggcaccgg
      781 atatctttcc accggtggcg ccagcttttt ttgctgtttc gtatatttct tccatcaaag
      841 gattgcttat cccttctgcc atttgtcgtt tttgctgaaa accataatcg agtatttcac
      901 caatttcatt cagccttcct ttcagcaaag cctctttcat catcctggcc tgctccttca
      961 gatgatgcat ggcttcaata gatttttctt tattatctgt aacgttcttg ctttgtttcc
     1021 tgatgatctc tgccgattca cgactggtgg aagtgtagta taataccagg ttattttcca
     1081 gttcaaacag atgttgctgc tttacccgca acgggttaac gatcactttg tcatctgcat
     1141 agaattccat aaaatttaca ccgccgaaag tggccgcata ctggtcttgc ctgcctccgg
     1201 ccaggttcag gtcccttctt tcaatttcat aggcataatg cgccatatca tattccccca
     1261 atggaagcct gagcatttct gcaaaagccc cgatgattgc tactacaaga gtggacgatg
     1321 ttcctaatcc tgaaccagca ggtgcatcca cataggttga taaacgaaag ccctgttctt
     1381 ttatcccata atctttttgg atacgattgt atacaccttt taacaggtca agtgtgcctg
     1441 tgacaggtaa ttcttttgtc cagtcaaatt cttgtatctc attcctgtca atagcttcaa
     1501 gtatgatctt attcgctgct gtcggctcaa tagtggcgtt ggcataaaga gaaagagtgg
     1561 catttaaaat agctccgccg tacaggtcac tgtatgggct tacatccgtg ccgccaccgg
     1621 ctaatccaat ccttaaaggg gctttacttc tgtatatcat ttgctaactg agaaatagta
     1681 ccggccattg tggcccagct atatttttgt ttctcattgc gaagatgcgg aataaaataa
     1741 ttttcaccta attggtagaa gttcaggatg ccctctgcaa tcgcggcggg atttggttct
     1801 acgaccaaac caactttctt atcaggaacc aatgaaggca atccccccac attggtgacg
     1861 accattggtt tttcgaaatg ataggcgagg ggggttacac cgctttgcgt agcattgcga
     1921 tatggttgta ttactgcatc ggctgcgcag aggtaatatt ttacttcact gtcaggtata
     1981 aaatctgttt ttaaaatcag ccgatctttt atacccaatc tttcaatctg ctcctggtat
     2041 tgttttgcat cctcataaaa ttcccctgcc accagcagcc tgattcccga attctttatt
     2101 cttacatttg ccattgcttc aaacagcaaa tccagtcctt tgtatttgcg gataaaacca
     2161 aagaagagaa ttattttttc attatccgga agatgcagtt gttttcttgc gtcagctttt
     2221 gaaataatag ctccgaaatt atcgtacaat ggatgtgata ccaactgagc aggttttgtc
     2281 ttttcaaata ttcttaaatc actcatcact ttttcgctca tggtgataaa ggcatcacag
     2341 cttttcagaa aatattttgt gaaaggctta tcaccaaatc ttttttcgtg aggtatcaca
     2401 ttatctgcaa tgcaaatgat ctttgtatgc ctgttttttc tgactcgcct gagaattgtt
     2461 cccagggctg gccccataaa gggaagccaa aagcgaacaa tgatcatgtc tggtctttct
     2521 tttctaagtt tattaccaac tctgatccag ttgaatggat taacagagtt tattgctgaa
     2581 tgtataataa tacct
//
LOCUS       DAORTI010000270         2555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_60124_length_2555_cov_5.392000, whole genome shotgun sequence.
ACCESSION   DAORTI010000270 DAORTI010000000
VERSION     DAORTI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60124_length_2555_cov_5.392000"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..994
                     /gene="rfbA"
                     /locus_tag="PLO70_17160"
     CDS             134..994
                     /gene="rfbA"
                     /locus_tag="PLO70_17160"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQZ76251.1"
                     /translation="MKGIILAGGSGTRLYPITKAISKQLMPIYDKPMIYYPLSVLMMA
                     GINEVLIITTPEDNPQFKRLLGDGSKIGCRFEYAVQEVPNGLAQAFVIGESFIGNDKV
                     ALVLGDNIFYGTGMGKQLQQLKNVEGGYVFAYQVADPERYGVVEFDEKFNALSIEEKP
                     ARPKSHFAVPGLYFYDNSVVKIAKELKPSARGEYEITDVNKVYLETGKLKVAVLDRGT
                     AWLDTGTFDSLSDASEFVRVIEKRQGTKIGCIEEVAYRNNFISREQLLQLAGELSKSG
                     YGEYLKTIIN"
     gene            1005..2345
                     /locus_tag="PLO70_17165"
     CDS             1005..2345
                     /locus_tag="PLO70_17165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQZ76252.1"
                     /translation="MKIAVVGTGYVGLVSGTCFSETGNRVTCIDIDKAKVEKLNAGQI
                     TIYEPGLEKIFLRNIKEERLHFTTSLEEGIKEASVIFLALPTPPDEDGSADLKYILGV
                     AKELGKLLQPGDYKVIVDKSTVPVGTAEKVKQAILDSSAGNIADTFDVVSNPEFLREG
                     AAVEDFMKPDRVVIGTSSEKAASIMRQLYEPFVRQGNPIIFMDEKSSELTKYAANSFL
                     AAKISFMNEIALLCERVGADVDMVRKGIGTDFRIGKHFLYPGLGYGGSCFPKDVQALS
                     KSSKDAAYEFKILNAVMEVNKRQKDFFIEKIKNYYGNDIKGRRFALWGLAFKPNTDDI
                     REAPALDVIDFLVSGGAVVTAYDPEAMPNVQAHYHNKNGSIGFSDSQYIALDGADALI
                     ICTEWNEFRTPDFEQMIKRLKAKVIFDGRNLYDTTSMSLLGFHYESIGRRKVVP"
     gene            2400..>2555
                     /locus_tag="PLO70_17170"
     CDS             2400..>2555
                     /locus_tag="PLO70_17170"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ76253.1"
                     /translation="MKRVLITGAAGFLGSHLCDRFIKEGYEVIGMDNLITGNLKNIEH
                     LFPLKEFS"
BASE COUNT          789 a          460 c          641 g          665 t
ORIGIN      
        1 atcagtcact tttgaagaag gtttatcaca aacaattgat tggtacctgg agaatgaaga
       61 atggttgaag catgttactt cgggggagta tcagcagtac tataaagagc aataccacaa
      121 aagttaacta gctatgaaag gaatcatttt agccggcgga agtggcacca ggctgtatcc
      181 aattacaaaa gcgatcagta aacaactgat gccgatctat gataagccga tgatctatta
      241 tccgctatcg gttttaatga tggcagggat caatgaagta ctgatcatca ctactcctga
      301 agacaacccg cagttcaaaa gattattagg tgatggcagt aaaatcggtt gccgctttga
      361 atatgccgtt caggaagtac ccaatggcct ggcccaggca tttgtcatcg gtgaatcttt
      421 tattggtaat gacaaggttg cgctggtcct tggtgataat attttttatg gaaccgggat
      481 gggcaaacaa ttgcaacaat tgaaaaatgt ggaaggcgga tatgtatttg cttaccaggt
      541 agctgatccg gaaagatacg gggtggtgga atttgatgaa aaatttaatg cgctgagtat
      601 agaagaaaaa ccagcccgtc caaaatctca cttcgctgtt cccggtttgt atttttatga
      661 taacagtgta gtgaagattg ccaaagaact gaaaccttcg gcaagaggtg aatatgaaat
      721 taccgatgta aataaagtat acctggaaac tggtaaacta aaagtggcgg tgctcgacag
      781 gggaacggcc tggctagata caggtacctt tgattcgttg agtgatgcca gtgaatttgt
      841 tagagtgatc gagaaaagac aaggaaccaa gatcggctgt attgaggaag tggcataccg
      901 gaataatttt atttcacggg aacagttact gcagcttgcc ggtgaactct ccaaaagcgg
      961 gtacggggaa tatttaaaaa caataatcaa ttaaaccaaa ctaaatgaaa attgcagtag
     1021 tcggaacggg ttatgttggc ctggtcagtg gtacctgctt ttccgaaacg ggaaacaggg
     1081 taacctgtat tgacatagat aaagctaaag tggagaagct caatgcaggc cagattacga
     1141 tctatgaacc aggtctcgaa aaaatatttt tacgaaatat caaagaagaa aggcttcatt
     1201 ttacgacctc attggaagag ggaataaaag aggccagtgt gattttcctg gcgctgccca
     1261 ccccacccga tgaggatggc agtgcagacc tgaaatatat tctcggggtg gcaaaggaat
     1321 tgggcaaatt attacaaccg ggagattaca aagtaatcgt ggacaaaagc actgtgccgg
     1381 tgggcacagc cgaaaaagta aaacaggcaa tactggacag cagtgccggt aatattgctg
     1441 atacatttga tgtcgtaagc aacccggagt ttttgaggga aggggccgct gtagaagact
     1501 ttatgaaacc cgaccgggta gttatcggaa ccagttccga aaaagcagcc agtataatgc
     1561 ggcaactata cgaacccttt gtgcgccagg gtaatcctat tattttcatg gatgaaaaaa
     1621 gttctgagtt gaccaagtac gccgccaata gtttcctggc tgccaagatt tccttcatga
     1681 atgaaattgc attgctctgt gaaagggtgg gtgctgatgt ggatatggta cgaaaaggta
     1741 ttggtacaga tttcaggatc ggtaaacatt ttctataccc gggcctgggt tatggcggca
     1801 gttgttttcc caaagacgtg caggcattgt ccaaatcgtc gaaagatgcg gcgtatgagt
     1861 ttaagatttt gaatgcagtg atggaagtga ataagcggca aaaggatttt ttcatagaaa
     1921 aaatcaaaaa ctattatggc aatgatatca agggccggcg ttttgccttg tggggattgg
     1981 cttttaaacc caatacggat gatatccggg aagccccggc attggatgtg atcgatttcc
     2041 tcgtcagcgg gggtgctgtt gtaacagctt acgacccgga agcaatgcct aatgtacaag
     2101 cacattacca taataaaaat ggttcaatag gtttttccga cagccagtat atagcgttgg
     2161 atggagcaga tgccctcatc atttgtaccg agtggaatga gttccgtaca cccgatttcg
     2221 agcaaatgat aaaacggctt aaagcgaaag ttatttttga tggtcgtaac ctgtacgata
     2281 ctacatctat gagcctgctc ggatttcatt atgaaagtat tggccgccgg aaagtagtgc
     2341 cctgatcaga ccaggtagcg gagcaggctt aaattttatc ttcattcata aatcaccaca
     2401 tgaaaagagt acttattacc ggggcagcgg gttttttggg ttcgcattta tgcgaccggt
     2461 ttatcaaaga aggatatgaa gttattggga tggataattt aataaccggc aacctgaaaa
     2521 atatagagca tttatttcca ctgaaggaat tcagt
//
LOCUS       DAORTI010000271         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_62598_length_2492_cov_4.630283, whole genome shotgun sequence.
ACCESSION   DAORTI010000271 DAORTI010000000
VERSION     DAORTI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62598_length_2492_cov_4.630283"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1184)
                     /locus_tag="PLO70_17175"
     CDS             complement(147..1184)
                     /locus_tag="PLO70_17175"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQZ76254.1"
                     /translation="MRNVVHTFKLSPDWKLIQAISRLDRFDASWAAIAKREGTSLRQL
                     KSIATVRSVGASTRIEGSAMSDEEVKVLIEKLSISKLEDRDSQEVAGYFEALDLISDE
                     YPNMDVSEGTIRNLHNLLMKHSLKDGWHMGNYKKHSNAVEAARPDGSKQVIFETTPPG
                     FATEDAMRALIEWWDTDTETHPIVKCALFCYDFVSIHPFQDGNGRLSRLIATLLLLKN
                     GYSWIQYVSLEHEIENRKTQYYQELMHSQKQRPGEDVYNWVLFFLDCLNNISGQLMTK
                     LCAQGTVASLGPKEKSVYLFIENHPGSRSGDIAEKLDIGLPTVKKILTALVQQKLIVK
                     HGAGAGTNYSL"
     gene            complement(1744..2079)
                     /locus_tag="PLO70_17180"
     CDS             complement(1744..2079)
                     /locus_tag="PLO70_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76255.1"
                     /translation="MKFFLIIIVTVFTIYSCKENRNHEKNGTHSLKQKLIGKWGGLDE
                     SSPVLQFTVDSIYYFDRNKTYSYQINNNDIIINFPENKGILKNVSVVIDTMIFYDDLP
                     EPVKAFRFK"
     gene            complement(2096..>2492)
                     /locus_tag="PLO70_17185"
     CDS             complement(2096..>2492)
                     /locus_tag="PLO70_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76256.1"
                     /translation="HLEDEFLFTGLEFLEKLTEILGKISNYNKGTFNPSEDPSIKKDL
                     DELKKMLSSYEENKTPANTPSNSTPKIEPGKSKINPVFLKPNGVVYDRGNGLKTIKTK
                     HGRQITNKPATDTFPAQKGYDFHHPLDKN"
BASE COUNT          693 a          532 c          446 g          821 t
ORIGIN      
        1 ttgttacagc tattccagtt tcccaatgtc gcctgccaaa acacttaccc tcactagggc
       61 agacatggga ggaacggtta ggggtggggc ggtgggagac actgggcgga aaaaaatcaa
      121 gtaacctggc ataacttctt tcggctttaa agggaataat ttgtacctgc gcctgcgcca
      181 tgttttacaa taagcttttg ctgcacaagt gccgtcagta tttttttaac tgtcggtaat
      241 cctatatcca gcttttctgc aatatctcct gaccggctgc cgggatgatt ttctataaac
      301 agataaacac ttttttcttt tggtccgaga ctcgctactg taccttgcgc acataatttt
      361 gtcatcaact gcccgcttat attgttcagg cagtcgagga aaaaaagcac ccaattatag
      421 acatcctctc cgggcctttg tttttgacta tgcatcagtt cctgataata ctgggtcttc
      481 ctgttttcta tttcatgttc caggcttaca tattgtatcc aactgtaacc attctttaat
      541 aaaagtaatg ttgcaatcag tcggctcaac cggccattgc catcctggaa aggatggatg
      601 ctgacaaaat catagcagaa taaagcacac tttacaattg gatgtgtttc ggtatcagta
      661 tcccaccatt caatgagtgc ccgcatagca tcttcagtcg caaaaccggg cggcgtagtt
      721 tcaaaaatca cctgcttaga accatccggc cgggccgctt ccaccgcatt actgtgtttt
      781 ttataattgc ccatatgcca gccatcctta agactgtgct tcatcaggag attatgcagg
      841 tttctgatag ttccttccga aacgtccata tttggatatt catcggaaat cagatccaac
      901 gcctcgaaat atccggctac ctcttgggaa tctctgtctt caagtttgga aatactgagc
      961 ttttcgatta gcaccttaac ctcttcatca gacattgcag acccctcgat cctggtggag
     1021 gcacctacac ttcggactgt agcgatagac tttaattgtc taagacttgt tccttccctt
     1081 tttgctatag cggcccaaga agcatcaaag cgatcaagcc tactgatagc ctgtatcagt
     1141 ttccagtccg gcgaaagttt aaacgtatgc actacattcc tcatgttata aaggtaagaa
     1201 aatatccgat tttatccgat tttatccgac ctttatccga ttcactaaat acccgatttt
     1261 atccgtttag agacgattga tagccaatat agtctgccgg gacagcacga ctgaatatga
     1321 catggcgagc agagtttagt gaaagggagc aaataaaccg cttacacttt tacaagtacc
     1381 tgcggcttct tagcttttat ttgtttgcag cgattatgta ctgacgaaaa aatgtggttt
     1441 attggatttg attttcattt aatacgaaat ttttacggct cttatggagc cgatacttcg
     1501 ttgcaaacga agtataacat cctataattt ttattttgct tactggttac aaactaccga
     1561 caaccgggtt acccgttgca tcccgcacat agctgggata ggtaaaacaa aaaaaaccgc
     1621 tgatcactta aacggtttaa ctttcttttt attcatcttc ccttacgtgg ctgtaagcca
     1681 cattgtaaac atacgaagcg aagacgcttc gtatagcctc actacatgct tcgaagagct
     1741 gggctattta aaccgaaaag ccttaacagg ttctggcaaa tcgtcataga atatcattgt
     1801 atcgattacg actgaaacat ttttaagtat gcccttgttt tctgggaaat ttataataat
     1861 atcattattg tttatttgat agctatatgt tttatttcta tcaaagtaat aaattgagtc
     1921 gacagtaaat tgtaaaacag gactactttc gtctaaccct ccccatttcc cgattagttt
     1981 ttgttttaaa gagtgagttc cattcttttc atgatttcta ttctctttgc aactataaat
     2041 agtaaaaaca gtaacaatga ttattaaaaa aaatttcatt ttatagattt aaaatttaat
     2101 tcttatcaag aggatgatga aagtcatacc ccttttgcgc cggaaaagta tcagttgctg
     2161 gcttattggt aatctgacga ccgtgttttg tttttatagt tttcaatcca ttacctctat
     2221 cgtatacaac accatttggt tttaaaaaaa ctggattgat tttacttttc cccggctcaa
     2281 ttttcggcgt agaattgctc ggtgtgttag ccggagtttt attttcttca taacttgaaa
     2341 gcatcttttt cagttcatcc aaatcttttt taattgatgg gtcttcactc gggttaaatg
     2401 tccccttgtt ataatttgat attttcccta aaatttcagt caatttttca agaaactcta
     2461 aacctgtaaa taaaaattca tcctccaaat ga
//
LOCUS       DAORTI010000272         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_62767_length_2488_cov_6.465680, whole genome shotgun sequence.
ACCESSION   DAORTI010000272 DAORTI010000000
VERSION     DAORTI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62767_length_2488_cov_6.465680"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..>2488)
                     /locus_tag="PLO70_17190"
     CDS             complement(118..>2488)
                     /locus_tag="PLO70_17190"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQZ76257.1"
                     /translation="LLVFSAMGQDKTISGILTGNVMDSKNKALEGASVQLISFSDTLN
                     KRSLTTDKAGEFTFSSIQFGYYKLSISYVGLQVLTIDSIHFRTERFDFNLTDIVLKPK
                     STELLEDVVIYAEKPLIQSKDGNITFNAGESALAAGSNASDLLTQVPLVAKDADGKIS
                     VRGKEPKILIDDKPVELNLQQLQDLLESLPGSSIEKIEVMTNPPPQYANEQGGVINIV
                     TKKGKVGRSGRISMSGGTRGEVSLNGSFNYRKQGLAISINAGTGYNRFLGNGYSIRNN
                     IYADSSNFFNTTNNYTNKSWRPSFRANVDYDINKNNLLNMVLSFNQNNFDNNNITEFK
                     NINRYDELWRLSQRTVSSSGDSYSPNLSIAYTLKGKPGETLKFISSFNFSMNENDRDF
                     YQQFFNPDYTPNGLDSMQRQESNTRINGHTFRLNYDKMLGNKKTFISVGSYYNRSNNH
                     VLVDASYKKKPEGTMEALALLSNDFWFHQTVGNLRGSVKQILGENFSFTLGTSAEKTN
                     IWFELLKEGRDVKNDYLTWLPFANINKTWKEKLSLTFAYRRSINRPGINHLNPTIDFS
                     DPYNVRFGNEKLEASTAHNFDFIAGRTKSKYFLNLGVGYNIVEDVFSQVRTLLEEGKT
                     QITWENISGRKEYEISSWNGITLSKKLRVNASASYTYSMYSEFDKTVRKFRDGGSFTS
                     NLNSNFTPTDVWSFTSGFNLNRFASPQGFARWNTSMNLGIQRKLFNKKLVVTANTIDP
                     FANQQRRTFTYGTNFNLENFSLTQTRNYRLTLAYNFNSQQKKTVPKLSK"
BASE COUNT          720 a          531 c          453 g          784 t
ORIGIN      
        1 ggaatcaaaa ggaagataat aagaaaaccc tgaaattaaa attctttatt tagccttatt
       61 aacctattct aaatcgttac ggaatcttgt taaacattcc tgtagtatat aaagaaatta
      121 tttagacagt ttaggcacag ttttcttctg ctggctgtta aaattatacg ccagggtcag
      181 cctgtaatta cgggtttgtg taagactgaa gttttcgaga ttaaaattag taccatacgt
      241 aaaggtccgc cgttgctggt tggcaaaggg gtcaatggta ttggcggtga ctacaagttt
      301 cttattaaat aactttcgct gtatacccag gttcatgctg gtattccacc gggcaaaacc
      361 ctgcggactg gcaaatcggt ttaaattgaa accgctggta aaactccata catctgtggg
      421 tgtaaaattg gaattcagat tagaggtaaa agaaccacca tccctgaatt ttctaaccgt
      481 tttatcaaat tcgctgtaca tactataggt ataactggca ctggcattaa cccgtaattt
      541 tttagataag gtgatgccat tccagctgct gatttcatat tcttttcggc cgctgatatt
      601 ttcccaggtg atctgtgttt tcccttcttc cagcaatgtt cttacctggc tgaaaacatc
      661 ctccacaata ttgtaaccga cacccaggtt caggaagtat ttcgattttg tccggccagc
      721 aataaaatca aaattatgcg ccgtggaagc ttcaagcttt tcattaccaa aacgtacatt
      781 atacggatca gaaaaatcta ttgtaggatt aaggtgattg atcccgggcc tgttaatact
      841 tctcctgtaa gcaaatgtca ggctcaattt ttctttccat gttttattga tattggcaaa
      901 aggcagccag gtcaggtaat cattctttac atcccttcct tctttcaata actcaaacca
      961 tatatttgtt ttttcggcag atgtgccgag agtaaaactg aaattctccc ctaaaatctg
     1021 cttaacagaa ccccgcagat taccgacagt ctggtgaaac caaaaatcat tactaagcag
     1081 cgccaatgct tccatggtgc cttcaggttt ctttttataa ctggcatcta ccaaaacatg
     1141 attgttggac cggttataat atgaacccac agagataaac gttttcttat tacccagcat
     1201 tttatcgtaa ttcaaacgga atgtatgtcc attgatcctg gtattgcttt cctgacgttg
     1261 catggaatca agaccattgg gagtataatc aggattaaag aactgctggt aaaaatccct
     1321 gtcattctca ttcatcgaaa aattaaaact gctgatgaac tttaatgttt ctcccggctt
     1381 tccttttaag gtataggcaa tactgagatt gggactgtaa ctatcccccg aactgctgac
     1441 agtgcgctga cttaaccgcc atagctcatc atagcggttg atatttttaa actctgtgat
     1501 attgttatta tcaaaattat tctggttaaa acttaaaacc atgttcagca gattattctt
     1561 attaatatca taatccacat tcgccctgaa actggggcgc cagcttttgt ttgtatagtt
     1621 attagtggta ttgaaaaaat tggatgaatc agcataaata ttattacgga tagaataccc
     1681 gttaccaaga aaacgattat acccagtacc ggcattgata ctgatggcca gcccctgctt
     1741 cctgtaatta aaactcccat tcagcgaaac ttcaccccgg gtgccacccg acatagaaat
     1801 ccttccactc cttcctactt tccctttttt tgtaacgata ttgataacac ctccctgctc
     1861 gttggcatat tggggtggcg ggttggtcat cacttcaatt ttttcaattg aactaccggg
     1921 taatgattcc agcaagtcct gtaattgctg caaattcaat tctaccggtt tatcatcaat
     1981 aaggattttg ggttccttcc cccttactga aatttttcca tctgcatctt ttgcaaccaa
     2041 cggcacttgg gtaagcaggt cgcttgcatt gctgcctgct gccaatgcag actcacctgc
     2101 attaaaagta atattgccat ccttggattg aatcaggggt ttttcagcat agatgaccac
     2161 atcctccagt aattctgtgg attttggttt cagtactata tccgtaagat tgaaatcgaa
     2221 acgttctgta cggaaatgaa tactgtcaat ggtcagcacc tgcaagccca cataactgat
     2281 ggaaagttta taataaccga attgaataga tgaaaaagta aactcgccag ccttatccgt
     2341 cgtaagggac cgcttattca gtgtatcaga aaacgagatc aattggactg atgcgccttc
     2401 caatgcttta ttcttactat ccataacatt tcctgtaagg atacctgata ttgttttatc
     2461 ctggcccata gcactaaaaa ccagtaaa
//
LOCUS       DAORTI010000273         2483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_62966_length_2483_cov_5.724876, whole genome shotgun sequence.
ACCESSION   DAORTI010000273 DAORTI010000000
VERSION     DAORTI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62966_length_2483_cov_5.724876"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /gene="asnB"
                     /locus_tag="PLO70_17195"
     CDS             <1..1853
                     /gene="asnB"
                     /locus_tag="PLO70_17195"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQZ76258.1"
                     /translation="TLREMTDAMHHRGPDDAGYEIFESTDFTIGFGQRRLSILDLSPL
                     GHQPMHFGDLVVNFNGEIYNFKEIRKELEDKGYSFNSWSDTEVILKGYHCWGLDVLQK
                     FIGMFAIALYDKSRQKIFLIRDRAGIKPLYYYRKDDLLLFGSELKALYKHERFEKKID
                     VNSLALFLQYSYIPGQYSIFQNTAKLLPGHILTVDLKSGSQEIIKYWDVFDYYNKPKL
                     DLSDADAISETDKLLKSAYEYRMVADVPVGIFLSGGYDSSSVAALLQTGRTEKLKTFT
                     IGFHENEFNEAPEAKKIAAYLGTDHTEWYLTAKEAGEVLPLLPEIYDEPFADNSTVPT
                     VLVSKLARQQVKVALSADGGDEIFGGYNKFNQAEKFTTSMPVALQTMLSGAMSLINPS
                     YIPYFNTKYNFSTRYEKMKLIWRKHDPVQAVKYISQYITESDVESFLGKPFERYHTSF
                     DDGDKLVKNDGVTNRLLAVDYKTFLVDNNLVKVDRATMSVSLEGREPMLDHRVVEFLS
                     RLPSSFKIRNGVNKWLLKEVVHKYIPKELMERPKRPFIAPLMVWFRDDLKEQLRYYLS
                     EESLSKSGVFDHKPIVRLRDEYLAGKKVNYQKLWQVLIFQLWYDKWMEKL"
     gene            1873..>2483
                     /locus_tag="PLO70_17200"
     CDS             1873..>2483
                     /locus_tag="PLO70_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQZ76259.1"
                     /translation="MKKNVLVTGGAGFIGSHVVRHCMAMGFEVVVLDDLSGGFEDHIP
                     EGASFVQGSVTDHELVTKLFNQYRFEYVYHMAAYAAEGLSHFIRRFNYNTNLVGSINL
                     INESVKHKVKCFVFTSSIAVYGKGQLPMSEDMTPEPEDPYGVSKYAVELDLRSAHEMF
                     GLNYVVFRPHNVYGENQNIGDKYRNVIGIFMNQIMQGKQLTIFG"
BASE COUNT          756 a          421 c          577 g          729 t
ORIGIN      
        1 caacactcag ggagatgact gatgctatgc atcaccgggg tcctgacgat gctggttatg
       61 aaatattcga aagtacagat ttcactattg gtttcggcca gcgcaggtta tcgatacttg
      121 atctttctcc gttaggccat cagcccatgc atttcggcga cctggtagtg aattttaacg
      181 gggagatata taattttaaa gaaatcagga aagaacttga agacaaaggc tactcgttta
      241 attcctggtc agataccgaa gtaattctta aaggatatca ttgctggggc ctggatgtgc
      301 ttcagaaatt tattggcatg tttgccattg ccttatatga taagagcagg caaaaaatat
      361 ttttaatcag ggacagagcc ggtataaaac cattatacta ctaccgcaaa gatgatttat
      421 tgctttttgg ttcagagtta aaggccttgt acaaacacga gagatttgaa aagaaaattg
      481 atgtcaatag cctggcgctt tttctgcagt atagttatat acccgggcag tatagtatat
      541 ttcaaaacac agcaaagtta cttcctggtc atattctgac cgttgacctg aagtctggaa
      601 gccaggagat aataaaatat tgggatgttt tcgattatta caataaacct aaactggatt
      661 tatcagacgc agatgctatc agcgaaacag ataagttatt aaaaagtgct tatgagtaca
      721 gaatggtggc agatgtacct gttggaattt ttttaagcgg gggatatgat agctcaagtg
      781 tggccgcact tttacaaaca ggtcgaacag agaaactcaa gacgtttact atcgggtttc
      841 atgaaaatga gttcaatgaa gctcccgagg ctaaaaaaat agctgcgtat cttggcacag
      901 atcatacgga atggtacctc actgccaaag aagcagggga ggtattgcca ttgcttcctg
      961 aaatttatga tgagcctttt gctgataatt ccactgtgcc tacagtgttg gtcagtaagc
     1021 tggcccgtca acaggtaaaa gttgccctta gtgcagatgg cggtgatgaa atctttggtg
     1081 ggtataataa atttaaccag gctgaaaaat ttaccaccag catgccggtt gctttgcaga
     1141 ccatgctgag cggtgctatg tcattgatca acccttctta tattccttat tttaatacga
     1201 agtataattt tagcacccgg tatgagaaaa tgaaacttat ctggagaaag catgatccgg
     1261 tacaggctgt aaagtatatt agccagtata tcacagaaag tgatgtagaa tcatttcttg
     1321 gaaagccatt tgagaggtat cacacttctt ttgatgatgg ggataaactg gtaaagaatg
     1381 acggtgttac aaacaggctg ctggcagttg attacaagac ttttcttgtg gacaataacc
     1441 tggtaaaagt tgacagggcc actatgtcgg taagtttaga agggcgggaa ccaatgcttg
     1501 atcacagggt cgttgaattt ttatccaggc tcccttcttc gtttaaaata agaaatgggg
     1561 ttaataagtg gttattaaaa gaagtagttc ataaatatat ccccaaagaa ttaatggaaa
     1621 gacccaagcg tccgtttatt gcaccattaa tggtttggtt tcgggacgat ttgaaagagc
     1681 aactgcggta ttatctgagc gaagaaagtc tttcgaaatc aggggtgttt gatcataaac
     1741 ctattgtcag attacgggat gaatacctgg ccgggaaaaa agttaattat cagaaattat
     1801 ggcaggtgct tatatttcaa ctgtggtatg acaagtggat ggaaaaatta taattcattt
     1861 aaaagtaaga atatgaaaaa aaatgtactg gtaaccggcg gcgcaggctt cattggctcg
     1921 catgtggtac gtcattgtat ggcaatgggc tttgaagtag ttgtacttga tgatttaagt
     1981 ggtggatttg aagatcatat cccggaaggt gcaagttttg ttcagggcag tgttactgac
     2041 catgaactgg ttacaaaatt attcaaccag taccggtttg aatatgttta tcacatggca
     2101 gcatatgccg ctgaagggct ctcacatttt attcgccggt ttaattataa taccaacctg
     2161 gtcggaagca tcaatttaat taatgagtcg gtaaagcata aggtaaaatg ctttgtgttt
     2221 acttcttcca ttgcggttta tggtaaagga caattgccaa tgagtgaaga tatgaccccg
     2281 gaacctgaag acccgtatgg tgtttctaaa tatgcagtgg aactggacct ccggtctgcc
     2341 catgaaatgt ttgggcttaa ttatgttgtt ttccgtcctc acaatgtcta tggtgagaat
     2401 cagaacatcg gtgacaaata caggaatgtg attggcatat ttatgaacca gatcatgcag
     2461 ggaaaacagc tgactatttt cgg
//
LOCUS       DAORTI010000274         2469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_63557_length_2469_cov_5.020713, whole genome shotgun sequence.
ACCESSION   DAORTI010000274 DAORTI010000000
VERSION     DAORTI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63557_length_2469_cov_5.020713"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="PLO70_17205"
     CDS             <1..2410
                     /locus_tag="PLO70_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76260.1"
                     /translation="GGKAAAGQTPPAAPGYGVQIGSSCFFTQSPIMGSVGSYKLVKTT
                     GNIIINGNIYSGETIQLANGNEIKGNLAAGSSPSGVPGTIVSIGSNAIIGISGDPAKG
                     NINANGKVTVSGGTVYGKVTVPAPQSIYPYTGPAPTGGVVYGTPTLPTLPQMPAITNF
                     PAFGTTNITSTTTITPGVYGAINLGNNKTLTLNGPGVYVFKSIKTTGPNSKIVYNFNN
                     SSSGNFLIYVHGDIILNKASFSMINGGSATRIYVETHGTGSTNTNNNTAAFDMSNGSN
                     GTGNPSGWLGSLWAPYAAIKAGSPNGPSSTVVGAFWSGTQVNIQGGVTFTFAPFSLCT
                     PPDVNAGPDKPLDFENPTILTGTSATPGVTFNWQAIDGGVITTPANAASITVSAAGSY
                     ILTATSTAGCFKKDTAIVTGKVNDIIGSELEAVLQNYNAGGTPSPFFEIQNEKIFIDV
                     IVKVGEYANVKAILTNPTGPWGMTNLLGEDASNFIITGLFPISKLADLNTDPVLALKI
                     VYARPYYRAYNNTIYPTGIISQGDTSVRTHLVRNGYNLSGEGVKIGVLSNSFNTITAA
                     TTNPVISSTAAQDIANNDLPGPGNPNNTTPVTVIKESPFVRSDEGRAMLQIIHDIAPK
                     AQLFFRTGVETANDFAAGIRQLKAAGCDIITEDITYITEPFLKDGVVAAAVDEVTAAG
                     TTFISSAGNFANKSFELPYTPMTAPGILNGTAHDFSGGDHRVVDVQEVCRCSEGPDPC
                     QHHRVRQDAVVHDAGAGRCQRGDGAVSVVGLARGQCRGTEGLPEHPQGRPPEERGRPD
                     AIAR"
BASE COUNT          703 a          537 c          553 g          676 t
ORIGIN      
        1 tggtggtaaa gccgctgccg gacaaacacc tcccgcagct cccggttatg gtgttcagat
       61 cgggtcttcc tgctttttta ctcaaagtcc cattatgggt tctgtaggaa gttataaatt
      121 ggtaaaaact accgggaata tcatcatcaa tggaaatatc tacagcggag aaaccattca
      181 gcttgctaat ggaaatgaga tcaaaggaaa cctcgctgca ggtagttcac catcaggtgt
      241 tccgggaact atagtttcta ttggatcaaa tgcaataatt ggcatcagtg gtgacccggc
      301 aaaggggaac attaatgcaa acggaaaggt taccgtaagc gggggtactg tctatggaaa
      361 agttacagta cctgctcctc aatccattta tccttataca ggtccggctc ctacaggtgg
      421 agttgtttat ggaactccca cacttcccac acttcctcaa atgccggcta taaccaattt
      481 tccggctttt ggaacaacaa atattacttc aacaacaact attactccgg gtgtttacgg
      541 ggctattaat cttggtaata ataaaacatt gacattaaac ggacccggtg tttatgtttt
      601 taaatcgatt aaaacaaccg ggcccaattc taagatcgtt tataatttca ataattcatc
      661 ttctggtaat tttcttattt atgttcatgg tgatataatc ctgaataaag caagttttag
      721 tatgatcaat ggaggatctg ctacccggat ttatgtggaa acacatggta ccggatcaac
      781 taatactaat aataatactg ccgcatttga tatgtcaaat ggctcaaacg gtacaggtaa
      841 tccatccggt tggttaggct cactatgggc gccttatgca gcgatcaaag caggatcgcc
      901 aaatggccca tcttcaactg tggtgggggc tttttggagc ggtacacagg taaatatcca
      961 gggtggggtc acttttacct ttgcaccttt ttcattatgt acaccgcccg atgttaatgc
     1021 aggtcccgac aaacctttgg attttgaaaa tccaacaata ttaacaggga catcagcaac
     1081 acctggtgta acctttaact ggcaggcgat tgacggcgga gtaataacta cccctgcaaa
     1141 tgctgcatca attactgttt cggctgctgg tagttatatt cttactgcta catcaacagc
     1201 cggttgtttt aaaaaggata cagccattgt aaccggtaaa gtaaatgata tcatcgggtc
     1261 tgaattggaa gcagtattac aaaattataa tgctggcgga acaccatccc ccttctttga
     1321 aatacagaat gaaaaaatat ttattgatgt gattgtcaaa gtaggagagt atgctaatgt
     1381 aaaggcaata ctgacaaatc caacaggacc ctggggtatg acaaatttgt taggtgagga
     1441 tgccagtaat tttattatta ccgggttatt tccaatcagt aaactggctg acctgaatac
     1501 ggacccggtt cttgcattga aaatcgttta tgcccgtccg tattaccgtg catataacaa
     1561 tacaatttat cctaccggta ttatttcgca gggagatact tctgttcgta cacacctggt
     1621 gcgtaatggt tataacctta gcggggaagg tgttaagatc ggagttttgt ccaatagttt
     1681 taataccatc acagctgcaa caaccaaccc tgttatcagt agtacagcag cacaggatat
     1741 tgcaaacaat gatcttcccg gtcccggaaa tccgaataat acaacgcctg taacagttat
     1801 caaagaatct ccttttgtac gctctgatga aggcagggca atgttgcaga tcattcatga
     1861 tatagctccc aaagcacaac tttttttcag aacaggtgta gaaactgcta atgattttgc
     1921 ggcggggatc cggcaactaa aagcggcagg atgcgatatc attacggaag atatcaccta
     1981 catcactgaa cctttcctta aagatggggt agtggctgct gctgttgatg aagtaactgc
     2041 agcgggtaca actttcattt cttcggcagg aaattttgcc aataaatcat ttgaacttcc
     2101 ttacacgcca atgacagcgc cgggtatttt gaatggaact gcacatgatt tttccggagg
     2161 cgatcaccga gttgtcgatg tacaagaagt ttgccgatgc agtgaaggtc ccgatccttg
     2221 ccaacatcac cgagttcggc aagacgccgt tgttcacgac gcaggagctg gccggtgcca
     2281 acgtggcgat ggcgctgtat ccgttgtcgg cctggcgcgc ggccaatgcc gcggcactga
     2341 aggtctacca gagcatccgc aaggaaggcc accagaagaa cgtggtcgac ctgatgcaat
     2401 cgcgagatga tctctacgat taccttgact atcattcgta cgagcagaag ctcgacgggc
     2461 tgttcaagc
//
LOCUS       DAORTI010000275         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_63936_length_2460_cov_5.943451, whole genome shotgun sequence.
ACCESSION   DAORTI010000275 DAORTI010000000
VERSION     DAORTI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63936_length_2460_cov_5.943451"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="ruvC"
                     /locus_tag="PLO70_17210"
     CDS             complement(<1..479)
                     /gene="ruvC"
                     /locus_tag="PLO70_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQZ76261.1"
                     /translation="MQKPVKIILGIDPGTIVMGYGLITVKGSEVNLLELGILKPGKVK
                     DGYKKLQLIFNTVSGLITKYQPTDFAIEAPFFGKNVQSMLKLGRAQGVAIAAAMRHGL
                     EVTEYSPKKVKQSITGNGNADKEQVMKMLQTLLSFKESPKHYDATDALAVALCHHFQT
                     "
     gene            505..1503
                     /locus_tag="PLO70_17215"
     CDS             505..1503
                     /locus_tag="PLO70_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HQZ76262.1"
                     /translation="MKLNRNIKIFINYFLGPLLFIWLSWSIYREIINQPDLTIAWQKI
                     SESFSSPMLWNLVTVVLLMFINWSIEAIKWKISVNEIQKVSFSRALKAVLSGVSFSVS
                     TPNRVGEYLGRVLYMEEGNRLKTISVTIAGSISQLIITLLMGLTGLIILRPAIEAEHF
                     VSPIWLTMIIYGVLAVLLVLTLFYFRLSWLIKWIDRLPGMKRFAYLVRVLEDFNATLL
                     LRILSLSLLRFFIFGIQYYLLFRFFDVDVSWWQAFWTVSVSFVVMAVIPTFAIAELGL
                     RGKVSLIFTGLFSANKAGIIFTTAGIWFINLIIPAIVGSLMILSIKKIYTEKNEGI"
     gene            1490..2296
                     /locus_tag="PLO70_17220"
     CDS             1490..2296
                     /locus_tag="PLO70_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQZ76263.1"
                     /translation="MKGFKVLVPLFFGLLGFSKNASVLPDTFCGINNTAFQVNEEVTF
                     TVYYSVAGVYVNAGTAIFTSKLEKVNNRPVYHVTGEGKTNSSYDWIYRVRDKYETYID
                     TATMQPLKFVRNVNEGGYKIYQNITFNKTANTAITTNGVFKVPACVQDVVSAMYYARN
                     IDFSKLRPEDKIPFSMFLDNEVYNMYIRYVGKEEIKTRYGKFRAIKIKPLLLKGQIFE
                     GGENMTVWVTDDANRIPVRIESPLVVGKVKIDMMSHSNLRHPLTSLVKRK"
BASE COUNT          688 a          446 c          532 g          794 t
ORIGIN      
        1 gtttgaaaat gatggcataa tgctacggct aatgcatcag tggcatcgta atgtttggga
       61 ctttctttaa aggacaataa tgtttgcagc attttcatca cctgttcttt gtcagcattg
      121 ccatttccgg tgatggattg tttcactttt ttaggagagt attctgttac ttccaggcca
      181 tgacgcatgg cggctgcaat agccactccc tgcgccctgc ccaatttcag catactttgt
      241 acattcttac caaaaaaagg ggcttcaatt gcaaaatcag tgggttgata tttggtaatt
      301 aatccactaa ctgtattaaa tataagctgt agttttttat acccgtcctt tactttacca
      361 ggctttaata tacccaactc cagtaaattg acttcacttc ctttaaccgt tattaatccg
      421 tagcccatga ctattgtgcc ggggtcgata ccaaggatta ttttaacagg cttttgcacg
      481 cagggatgta tttttacaag attaatgaag ctgaaccgaa atatcaaaat cttcatcaat
      541 tacttcctgg gtccgctact gtttatatgg ctgtcatggt caatttacag ggaaataata
      601 aatcagccgg accttactat tgcctggcaa aagatcagtg aatcgttttc cagtcctatg
      661 ctatggaacc tggtgaccgt tgtgttgctg atgtttatta actggtcaat tgaagccatt
      721 aagtggaaga tatctgtgaa tgagattcag aaagttagtt tttcaagggc attaaaagct
      781 gttttatccg gcgtgtcatt ttctgtaagt actcccaata gggtgggaga atacctcggc
      841 cgggtattat atatggagga gggcaacagg ctaaaaacaa tttctgttac gattgccggc
      901 agtatcagcc agcttatcat cactttgctg atgggattaa caggattgat cattctacgt
      961 ccggctatcg aagctgaaca ttttgtttct ccaatatggc tgaccatgat catttatgga
     1021 gtattagcag tattactggt tttaacacta ttttatttcc ggttatcatg gctcataaag
     1081 tggatagacc gtttgcccgg aatgaagcgg tttgcatacc tggtgagagt attagaagat
     1141 tttaatgcaa cattattact gcggatcttg tccttatcac tgctcaggtt ctttattttc
     1201 ggaatacaat attacctgct gttccgtttc tttgatgttg atgtttcctg gtggcaggct
     1261 ttttggacgg ttagtgtttc attcgtggtg atggccgtga ttccgacttt tgccattgca
     1321 gagttagggt tgagagggaa agtgagtctt attttcacag ggctattcag tgctaacaaa
     1381 gccggaataa tttttacaac agccggcatc tggtttatca acctgataat tccggcgatc
     1441 gtaggaagcc tgatgatcct gagcattaaa aaaatttata cagaaaaaaa tgaagggatt
     1501 taaagtttta gtgccacttt tttttggatt gctcgggttt tctaaaaacg caagtgtgct
     1561 gcctgatact ttttgtggta tcaacaatac agctttccag gtgaacgagg aagtaacatt
     1621 cactgtttac tattcagtgg cgggtgttta tgttaatgcc ggtacggcta tttttaccag
     1681 taaactggag aaagttaata accggccggt ataccatgtg acgggcgaag ggaaaacaaa
     1741 ctcttcttac gactggatct acagggtgag ggataagtac gaaacatata ttgatacggc
     1801 caccatgcag cccctgaagt ttgttcgtaa cgtaaatgaa gggggatata aaatatacca
     1861 gaacattact tttaataaaa ctgccaatac tgcaattact accaacgggg tatttaaagt
     1921 acctgcctgt gtgcaggatg tggttagtgc catgtattat gcccgtaata ttgatttttc
     1981 aaaattgcgt ccggaagata aaataccttt ttccatgttt ttggataatg aagtgtacaa
     2041 catgtacatc cgttatgtag gaaaagagga aattaaaacc aggtatggta agttcagggc
     2101 tattaaaata aagcctctgc tgttgaaagg acaaatattt gaagggggag agaatatgac
     2161 cgtttgggtg acagatgatg ctaaccgtat tcctgtcaga attgaaagtc cattggtagt
     2221 gggtaaagtt aagattgata tgatgagtca ttctaacctg cgtcatccgc tcacatccct
     2281 ggtaaagaga aagtaggtat aataatatta cacttcttca agtttaaaaa aatccccggg
     2341 tcgtatccct ctctctgttt gttgtaagtt acagcattca tgtaccgggt catgctcaaa
     2401 gaaaagaata taatctcctg ctattgcttc tttcaggaat gatttctttt catttaatgt
//
LOCUS       DAORTI010000276         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_65146_length_2432_cov_5.291544, whole genome shotgun sequence.
ACCESSION   DAORTI010000276 DAORTI010000000
VERSION     DAORTI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65146_length_2432_cov_5.291544"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLO70_17225"
     CDS             <1..650
                     /locus_tag="PLO70_17225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQZ76264.1"
                     /translation="IAKNYSKELVEKGKKKNKKELAGQFGLDPDKPLISFIGRLVGEK
                     AADLLPEAISQSIYQHHGHANFLVLGSGDPELEDRLDQMKHQFNGYYNSYIGYSEPLS
                     HLIYAGSDFLLMPSRVEPCGLNQMYALRYGTVPMVRNIGGLRDTVTDMGDWQGFGIRF
                     DQASVGDITYSVGRAIDLYNNKQDLYSWMRSHMMTIDHSWDSSAQQYIDLYKSLK"
     gene            662..1954
                     /locus_tag="PLO70_17230"
     CDS             662..1954
                     /locus_tag="PLO70_17230"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQZ76265.1"
                     /translation="MAIRTITHTKEILSVILGGGAGSRLYPLTASRSKPAVPIAGKYR
                     LVDIPISNCLNNGIGRMFVLTQFNSASLNRHIKNTYHFSAFSKAFVDILAAEQTPDNP
                     TWYQGTADAVRQGLRHIEPFESDYILILSGDQLYQMDFAEMLDNHKALGADISVATIP
                     VAQREAPEFGILKSDNGLITSFIEKPKKEVLGEWVSDTGLEMQNAGRNYLASMGIYIF
                     NRNLLFDLLRTEFKDATDFGKEIIPQSIIKYKVASYQYSGYWTDIGNIHSFFEANIDL
                     TKDIPDFNMFDSDRTIYTRPRMLPPGKISGTTLEKTLIAEGSIINASRVENSVIGIRS
                     RVGHGSTIVSTYIMGSDYYETLADIASAAEKGIPLLGVGDRCYIKNAILDKNVRIGND
                     VRINGGTHLENTDHALYTVKDGIVVIKKGAIVPNGFVI"
     gene            2067..>2432
                     /locus_tag="PLO70_17235"
     CDS             2067..>2432
                     /locus_tag="PLO70_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQZ76266.1"
                     /translation="MSTAIISGKPRLSLSQIINMSVGFFGIQFAFGLQNANVSRIFQT
                     LGAKIEEIPILWIAAPLTGLIIQPIIGHMSDKTWLGKWGRRRPYFMTGAIFASLALFV
                     FPNVSALWMAAVMLWLLDAS"
BASE COUNT          700 a          452 c          551 g          729 t
ORIGIN      
        1 tgatcgcaaa gaattatagt aaagaattgg ttgaaaaagg gaaaaagaaa aataaaaaag
       61 aattggccgg gcaatttggt cttgatcctg ataaaccatt gatctccttt attggaagat
      121 tggtaggcga gaaagcagca gatctgctgc ccgaagcaat aagtcaatcc atttaccagc
      181 atcatggaca tgccaatttc ctggttcttg gttctggtga tcctgaactg gaagacaggc
      241 tcgatcagat gaaacaccag tttaatggtt attacaactc ctatattggt tatagcgaac
      301 cgctgagcca tctaatttat gcaggttcag actttttgtt gatgcccagc cgggtggaac
      361 cttgtggttt aaatcaaatg tatgcccttc ggtatggaac agtgccgatg gttagaaata
      421 taggtggatt aagagatacg gtaactgata tgggcgactg gcagggcttt ggtatccgct
      481 ttgaccaggc atctgttggc gatattacct attctgtcgg aagagctatt gacctttaca
      541 ataataaaca ggacctttat agctggatgc gcagccatat gatgactatt gatcattcct
      601 gggattcatc tgcgcagcaa tatattgact tatacaaatc actgaaataa aaagatcggt
      661 tatggcaatc agaactatca ctcacacaaa agaaatctta tcagttattc tcggcggagg
      721 tgccggctcc aggttgtatc cgcttacagc cagtcgttca aaacctgctg tacctattgc
      781 cggtaaatat cgcctggtgg atatccccat ctccaattgt ttgaacaacg gtatcggtag
      841 gatgtttgtg ctgacacaat ttaattcagc atcactgaac aggcatatca aaaacactta
      901 tcattttagt gcattcagca aagcctttgt tgatatactg gcagcggagc agacgcctga
      961 taacccgacc tggtaccagg gtacggcaga tgctgtccgc cagggactcc ggcatattga
     1021 gccttttgaa agtgattata tacttatcct gtctggtgat cagttgtacc agatggattt
     1081 tgcagaaatg ctggacaacc ataaagcttt aggtgctgat atatctgtgg caacaattcc
     1141 tgttgcccag cgggaagcac cggaatttgg tattctaaaa agtgataatg gtttgatcac
     1201 ttcctttatt gaaaaaccaa agaaagaagt actgggcgaa tgggtaagtg ataccggtct
     1261 tgaaatgcaa aatgccggac gaaattacct tgcctcaatg ggtatttata tattcaacag
     1321 gaacctgctg tttgatttgt tacgaacaga atttaaagac gcaactgatt tcggtaaaga
     1381 gatcataccg caatcgatca taaaatataa agtcgccagt taccagtata gcggttactg
     1441 gacggatatc gggaatattc attctttttt tgaggctaat atcgacctga caaaggatat
     1501 ccctgatttc aatatgttcg atagtgaccg tacgatctat acaaggccga ggatgcttcc
     1561 tccgggaaag atcagtggca ctacgctgga aaagacatta attgctgaag gttcaataat
     1621 taatgcctcc agagttgaga attctgtgat aggtatccgt agccgggtgg ggcatgggag
     1681 cacaattgtc agcacttata tcatgggaag cgattattat gaaacattgg ccgatattgc
     1741 cagtgctgct gaaaagggta tccctttgct tggggtaggc gacagatgct atatcaaaaa
     1801 tgcaatcctt gacaaaaatg taaggattgg taatgatgtt cgtatcaatg gcggtactca
     1861 tcttgaaaat acagatcatg ctctttatac agttaaggac ggtattgttg ttataaagaa
     1921 aggggctatt gttccgaacg gttttgttat ttgatacgtt tttttattgt gcatcataaa
     1981 gaaataatta aggtacacaa caggtgtacc tttttcattt tattcctatc ttccctgtta
     2041 ctgattaaat taaaatatga ttgcttatgt caacagctat tattagtgga aagccccgcc
     2101 ttagtttgtc ccagatcata aatatgagtg ttggtttttt tgggattcag tttgcatttg
     2161 gccttcagaa tgctaatgtc agccggatat ttcaaacact tggtgctaaa attgaagaaa
     2221 ttccaattct ttggatagct gcgccattga caggtttgat cattcagcca atcataggtc
     2281 acatgagtga caaaacatgg ttgggtaaat ggggcagaag aaggccttat ttcatgaccg
     2341 gggcaatttt tgcttcgttg gctctttttg tttttcctaa tgtttcagcc ttatggatgg
     2401 ctgctgttat gctatggctg cttgacgcct ct
//
LOCUS       DAORTI010000277         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_71996_length_2293_cov_3.699732, whole genome shotgun sequence.
ACCESSION   DAORTI010000277 DAORTI010000000
VERSION     DAORTI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71996_length_2293_cov_3.699732"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLO70_17240"
     CDS             <1..474
                     /locus_tag="PLO70_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76267.1"
                     /translation="KLETLMEKSERQRRALQSVSTTGNSAFEINASNPFSLVFGSIVA
                     TFFRPFIWEINTPIALFSAVESFIFLILTLNFFIKKGIGVYFRSIFSDPKLLMCFVFA
                     VVFAVAVGASTTNFGALSRYKIPCMPFYFIMLLLVYKKASLPYPKWFSTLFKKIA"
     gene            478..>2293
                     /gene="asnB"
                     /locus_tag="PLO70_17245"
     CDS             478..>2293
                     /gene="asnB"
                     /locus_tag="PLO70_17245"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQZ76268.1"
                     /translation="MCGIAGSINFKLDIPVLTKALFHRGPDEQTTFEEHNLQLHHHRL
                     AILDIAGGHQPMHLGPYTIIFNGEIYNHLEVRKKFKLSCNSNSDTETILHAYAQLGSS
                     CLQEFDGMFAMAIYDRDKQELFLARDRAGKKPLYYYSEGQAFVFSSELNALRSQLDLQ
                     INETHIQQYVRMGYFYKSSTPYTNVSELPGGSYATVSLTQPRIRVTKWWNINSFYTKP
                     DTDNFATATEKVESILHEAVRNRVESSDLEVGSFLSGGIDSGLVTAIASQYNSSLKTF
                     TVSFDGEYDEAPLAKLVADRYQTAHHEIKISFDHLKDDIEKILSNYGEPFFDSSAIPS
                     YYVSEAAKKHLTVILNGDGGDEIFGGYRRYVPFARYDFFKPGGLVKMMAAIIHTVLPV
                     PNNKKSKYNYIYRLADLAGKGNLSAYLSSTIDSFEGFEKYLVSDMHYLDNVNKDFAEI
                     NGSHLSGLQKIMNLDFDNILTGNLLVKMDIATMAHSLEGRSPLLCKGLLEYVPAIKDE
                     YKIKGAQTKYILRKLAEKYLPAELINQPKRGFEIPLKKWIDGELKDMIASYILSPAAY
                     CKNFVQPGFIENLWNRKIKAGDEKRAKMLWTLFALEVWY"
BASE COUNT          674 a          424 c          482 g          713 t
ORIGIN      
        1 aagctggaaa cacttatgga aaaatcagaa cgtcaacgaa gggcattgca gagtgtaagt
       61 actaccggta attcagcttt cgagatcaat gcatctaatc cttttagctt ggtctttgga
      121 agtatagtgg ctaccttttt tcgtcctttt atctgggaaa ttaatacccc cattgcatta
      181 ttttctgctg tggagtcatt tatatttttg attctgacgc ttaatttttt tattaaaaaa
      241 ggaatagggg tttattttag atctattttt tctgatccta agttattaat gtgttttgta
      301 tttgcagttg tatttgctgt ggcagtgggg gcatctacta ccaattttgg tgctttgtcc
      361 aggtataaaa ttccctgtat gcctttttat tttattatgt tactccttgt ttataaaaaa
      421 gcatcattac catatcctaa atggttcagt actttattca agaaaattgc ctgatatatg
      481 tgcggaattg ccggttccat caactttaaa ttagatatcc ccgttctcac caaagccctc
      541 tttcaccgag ggccggatga gcagacgact tttgaagagc ataatcttca gctgcatcat
      601 caccggctgg ccatacttga tattgccgga ggtcatcagc ccatgcattt gggtccttac
      661 accattattt ttaatggaga aatatacaat cacctggaag taagaaaaaa atttaagtta
      721 tcctgtaact ccaactccga taccgaaact attctgcatg cttatgcaca acttggctcc
      781 tcctgtttgc aagaatttga tggcatgttt gcgatggcga tttatgatcg ggataaacag
      841 gaacttttcc tggcaagaga cagggccgga aagaagccct tgtattatta ttcagagggc
      901 caggcttttg ttttttcaag tgaactgaac gccttacgca gccagcttga tctgcaaatc
      961 aatgaaacac atattcagca atatgtgaga atgggatatt tttataaatc atcaactcct
     1021 tatacaaatg tgagtgagtt gcccgggggt agttatgcta ctgtttcgct gactcaaccc
     1081 cgtatccggg ttacaaaatg gtggaatatc aattcttttt atacaaaacc tgatacagac
     1141 aactttgcta ctgcgacaga gaaagttgaa agcattcttc atgaagctgt gcgtaacagg
     1201 gttgagtcct cagatcttga agtgggttct ttcctcagcg gaggtattga cagtggcctt
     1261 gtcactgcca ttgccagtca atacaattct tccttaaaga cattcacggt ttcatttgat
     1321 ggggaatatg atgaagcacc actcgccaaa ttggtggctg acaggtacca gacagctcat
     1381 catgaaataa aaatttcttt tgaccacctg aaagatgata ttgaaaagat actcagtaat
     1441 tacggggaac ctttttttga cagctccgct attcccagtt attatgtgag tgaggccgca
     1501 aaaaaacacc tgactgtaat attaaacgga gacggagggg atgagatatt cggagggtat
     1561 cgccggtatg tgccttttgc caggtatgat ttttttaaac ccggaggatt ggtaaagatg
     1621 atggcagcta taatacacac tgtattaccg gttccaaata ataagaaaag taaatacaat
     1681 tatatatacc ggctcgctga tcttgcaggg aagggcaacc tttccgctta cctgtcttca
     1741 acgatagata gttttgaagg gtttgaaaaa tacctggtaa gtgatatgca ttacctggat
     1801 aatgtgaaca aggattttgc tgagataaac ggatctcatc tttcaggttt gcaaaaaata
     1861 atgaatcttg attttgacaa tatcctgacg ggaaatcttc ttgttaaaat ggatattgcc
     1921 accatggccc attcgctgga agggcgcagc ccgttacttt gcaaagggtt actggaatat
     1981 gtacctgcta taaaagatga gtataagata aagggagctc agacaaaata tattctgcga
     2041 aagctggctg aaaaatattt acctgctgaa ctgattaacc agcccaaaag aggttttgaa
     2101 attccgctaa agaagtggat tgatggtgag ttaaaagata tgattgcttc atatattctt
     2161 tctcctgctg cttattgcaa aaattttgtt caacccggct ttatagagaa tctctggaac
     2221 agaaaaatta aagcaggcga tgaaaaaaga gctaagatgc tctggacatt atttgcgttg
     2281 gaagtgtggt acc
//
LOCUS       DAORTI010000278         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_72193_length_2289_cov_5.726500, whole genome shotgun sequence.
ACCESSION   DAORTI010000278 DAORTI010000000
VERSION     DAORTI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72193_length_2289_cov_5.726500"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLO70_17250"
     CDS             complement(<1..283)
                     /locus_tag="PLO70_17250"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76269.1"
                     /translation="MQQFWDQRYAENETVYGNEPNKFFRLFIDQHKPGTLLLPAEGEG
                     RNAVYAASRGWQVDAFDFSQVAKEKALDFARGERVVINYELKNIADFKAG"
     gene            394..960
                     /locus_tag="PLO70_17255"
     CDS             394..960
                     /locus_tag="PLO70_17255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQZ76270.1"
                     /translation="MKYLFSLIVLLGVLAGCSNSTTKESTATATEPANSSQPAIPEIV
                     PAPPPAVATSATALPAFRMVNTSNQVIDLSQFKGKKVFVNLWATWCPPCRAEIPSIES
                     LGAKTDKSKAVFVMLSLDDNFEKAKSFAAKNNMKLPVYYPAGNLPQLFVVDGIPVTYI
                     FNENGEIIYQQVGSADYDTKEFFDMLNK"
     gene            complement(1033..1407)
                     /gene="trxA"
                     /locus_tag="PLO70_17260"
     CDS             complement(1033..1407)
                     /gene="trxA"
                     /locus_tag="PLO70_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQZ76271.1"
                     /translation="MATVKLTTQDFKDKVFNYETEQDWKYLGQLPAIIDFYADWCGPC
                     KMVAPVLEELSKEYEGKLVIYKVNTDVEQELSAVFGIQSIPTMLFIGADGEPMMQPGA
                     YPKHIFKKIIEEKLLAPVKTEE"
     gene            complement(1463..>2289)
                     /locus_tag="PLO70_17265"
     CDS             complement(1463..>2289)
                     /locus_tag="PLO70_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQZ76272.1"
                     /translation="EKILVESGRADKLKMIYIETPANPTNDLIDIEMCSDLARKYSSS
                     GKKVMVTVDNTYMGPLWQHPLKHGADIVLYSATKYIGGHSDVIAGAAVGSKEVIGKIK
                     KTRTFFGTMIDPHTSWLLMRSLETLKIRMEAAAANAEKVAEYLSRHHKVEKVYYLGYT
                     AKNNPDQSGIFIKQYLSNGAMLSFDVVGGEHEAFTFLNSLKHIKLAVSLGGTESLAEH
                     PASMTHADVPFEEKQELHVTDKMVRLSIGIENYKDLIWDIEQALEKVKKPLLEAVM"
BASE COUNT          630 a          538 c          407 g          714 t
ORIGIN      
        1 tacctgcttt aaaatcagca atatttttta gttcataatt aatgacaacc cgttcacccc
       61 ttgcaaaatc aagcgccttt tctttggcta cctggctgaa atcaaaagca tctacctgcc
      121 agcctctgga agctgcatac acggcatttc tgccttcccc ttccgccggc aggagcagtg
      181 taccaggctt atgctggtct ataaaaagcc tgaagaactt attcggttca ttaccatata
      241 cggtttcatt ttctgcgtac cgctgatccc agaactgttg cattgtaaca agtttctaca
      301 aatttgagca ttgccaagca ggctttaggt gatatttatc accagccgta acagatcaga
      361 attgcatctt gcacttctaa acattttact caaatgaagt accttttttc attaattgta
      421 ttgcttggag ttcttgccgg ttgcagcaac tcaacaacaa aggaatcaac ggcaaccgct
      481 acagagcccg ccaattcatc acagcctgct ataccggaaa ttgttccggc tcctccgcca
      541 gctgttgcaa cttccgctac tgcactgcca gcatttcgta tggtgaatac cagtaaccag
      601 gttattgacc tatcgcagtt taaaggaaag aaggtatttg taaacctctg ggccacctgg
      661 tgtcctccct gccgtgcaga gataccctct attgaaagcc tgggtgctaa aacggataaa
      721 agtaaagcag tgtttgtaat gctatcgctg gatgataatt ttgagaaagc aaaatcattc
      781 gccgcaaaga ataatatgaa actgccggtt tactaccctg caggaaacct gccgcagttg
      841 tttgtagtgg atgggattcc cgttacatat atcttcaacg aaaatggtga gatcatttac
      901 cagcaggtag gaagtgctga ttacgataca aaagaatttt ttgatatgct gaataagtag
      961 tcaatgcctt tagaattaaa aataaaaatg ccccggaaaa ccggggcatt tttctatact
     1021 atatattatt atttattctt ctgtttttac cggtgccagt aatttctctt ctattatttt
     1081 tttgaagata tgtttggggt aagcacccgg ctgcatcatc ggttctccat cggcacctat
     1141 aaatagcatg gttggaatac tttgaatacc aaatacagct gagagttcct gctccacatc
     1201 tgtattcact ttataaatca ccagtttgcc ttcatattct tttgaaagct cctccaatac
     1261 aggggctacc attttacagg gaccacacca gtcagcatag aaatcaataa ttgcaggtaa
     1321 ctgccccagg tatttccagt cctgttctgt ttcgtagtta aacactttat ccttaaaatc
     1381 ctgtgttgtc aattttactg ttgccatatc aaataattta tacgaaaata gaacataaca
     1441 atatactggt tcgtgatgta aatcacatta ctgcttccag caacggtttc ttaacttttt
     1501 caagggcctg ctctatatcc cagataaggt ctttgtaatt ttcaatacca atgctcaacc
     1561 gcaccatctt atcagtaaca tgtaattcct gtttctcttc aaacggcaca tcagcatggg
     1621 tcatactggc aggatgctct gcaaggctct ccgtgccacc aaggcttacc gccagtttga
     1681 tatgtttcag actgtttaag aaggtaaatg cttcatgctc ccctcccact acatcaaagc
     1741 tcagcatagc accgttactc aggtactgtt ttataaaaat acctgactgg tccggattat
     1801 tctttgcagt ataccccagg taataaactt tttcgacttt atgatgccgg gataaatact
     1861 cagctacttt ttcagcattt gcagctgcag cttccattct tattttcaat gtttccaggc
     1921 tacgcatcag taaccagctg gtgtgcggat caatcattgt tccaaaaaac gtacgggtct
     1981 tttttatctt tccaatcact tctttgctac ccacggctgc acctgcaatt acatcactat
     2041 gcccgcctat atactttgtt gcagaataca aaactatatc agccccatgc tttaacggat
     2101 gctgccacag cggtcccata taagtattat cgaccgttac cattaccttt ttgccagatg
     2161 aagaatattt tctggcgaga tcactgcaca tttctatatc aataaggtca ttggtaggat
     2221 tggccggagt ttctatataa atcattttca atttgtccgc acggccactt tcaactaata
     2281 tcttttcca
//
LOCUS       DAORTI010000279         2287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_72312_length_2287_cov_6.569444, whole genome shotgun sequence.
ACCESSION   DAORTI010000279 DAORTI010000000
VERSION     DAORTI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72312_length_2287_cov_6.569444"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..717
                     /locus_tag="PLO70_17270"
     CDS             133..717
                     /locus_tag="PLO70_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76273.1"
                     /translation="MKRVLLLILAPTIISIACKDNKEQVEEKNFISVLSLIEQQVAHV
                     DTSLYAITKFVFSDSLHIDTFYIRREEFRTVAKDFLEIPDLSVKKIANRYKEETRFDE
                     LLNRVIISYLPLNPEKEEIQKQELFVTPNIATGDKVNTILISRVINNREGYLQKEMLW
                     QMDKSFQVVTTTQKPGEPEIITTTKVTWNENDDQ"
     gene            714..>2287
                     /gene="uvrC"
                     /locus_tag="PLO70_17275"
     CDS             714..>2287
                     /gene="uvrC"
                     /locus_tag="PLO70_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQZ76274.1"
                     /translation="MTGAQFQHIANTIPAQPGIYKYFDSHNELLYVGKAKHLRKRISS
                     YFTKTFTSYKTHELVKRIARIEFTIVGSEQDAFLLENSLIKQFQPRFNINLKDDKTYP
                     FIVIKNEPFPRVFLTRNKVNDGSEYLGPFTSVKKVRELLEFIKQTIQLRNCSLNLTDN
                     NIQKGKFKVCLEYHLGNCKGPCEGLQSTTDYKEDLDQLKNLLKGNLTPVIRHFKEEMK
                     ANAAALAYEKAELARKKIDFLENYQSRSVVANAKAGNLDIFSLLKEKDTAFVNYLMVR
                     NGSIIQTQTSRIETHLDESLEEILSFSVAWLRTTFNSDAAEIVVPFNIDYPQNEITVT
                     VPKSGDKKKLLELSEKNVSHFIEELNKNDRLKLDIKKADKFTLLVQLQKDLQLRELPI
                     HIECFDNSNFQGSYPVSAMVCFRNGEPSKKDYRRFNVKTVEGINDFATMKEAVFRRYK
                     RLLEEDQPFPQLVIIDGGKGQLNAAMEAITELKLNGKSTLVGLAKNEEEIFFAGDIAS
                     LKLPYNSGSLKFIRRI"
BASE COUNT          794 a          433 c          443 g          617 t
ORIGIN      
        1 aaatgggcat acagctcaac aagtagaact tacactaact gctgctttgc aaaaaaatcc
       61 cgtctcacct ccgcagacgg gatttttatt ttcagttgtt ccctataact gtgtttacat
      121 taattttagg ctatgaaaag agttctgtta ttaattcttg ctccaaccat tataagcatc
      181 gcctgcaaag acaataagga acaagtcgaa gaaaaaaact ttatctctgt gctatcgttg
      241 attgaacaac aggttgccca tgttgataca tccctttacg ctattacaaa atttgtattt
      301 tctgacagtt tgcatataga tacattttat atcaggaggg aagaatttcg caccgtagct
      361 aaggattttc tcgaaattcc cgatttatct gtaaaaaaaa ttgccaaccg gtataaggag
      421 gaaacccgtt ttgatgaatt actcaatcgg gttattatca gttacctccc tttaaaccct
      481 gaaaaagaag aaattcagaa acaggaactc tttgtaaccc ctaacatagc cacaggtgat
      541 aaagtaaata caattttaat aagcagggtc atcaataacc gggaaggcta tttacaaaag
      601 gaaatgctct ggcaaatgga taaaagcttt caggtggtga caacaaccca aaaacccgga
      661 gaaccggaaa ttattacaac taccaaagta acctggaatg agaatgatga ccaatgacag
      721 gtgctcaatt tcaacatata gctaatacta ttcccgcaca accggggatt tacaaatact
      781 ttgacagcca taacgaatta ctttatgttg gcaaggctaa acatcttcgg aaaaggataa
      841 gttcctattt caccaaaaca tttaccagtt ataaaactca tgaacttgtt aagcgaattg
      901 cacggatcga gtttacaatt gtcggctcag aacaagacgc tttcttgctc gaaaattcac
      961 tcattaaaca gttccagccg cggttcaata taaacctgaa ggatgacaaa acttatcctt
     1021 ttattgtcat taagaatgaa ccttttccaa gagtatttct tacacggaat aaagtaaatg
     1081 atggatcaga gtatcttggt ccctttactt cagtaaaaaa agtaagagag ttgcttgaat
     1141 ttataaagca aacgattcag ctgagaaatt gctccctgaa tcttactgac aataatattc
     1201 agaaaggcaa gtttaaagtt tgcctggaat atcatcttgg caactgtaaa ggcccctgtg
     1261 agggcttgca atcaactaca gattataaag aagaccttga tcagctcaaa aatcttctga
     1321 agggcaactt aacaccggtt attcgtcatt ttaaagaaga aatgaaagct aatgctgctg
     1381 cacttgccta tgaaaaagca gaactggcaa gaaagaaaat agatttcctt gaaaactatc
     1441 aatcacggtc agtggtagcg aatgcaaaag ccggaaattt ggatatcttt tctttactta
     1501 aagagaagga tacggctttt gtaaactacc tgatggtaag aaacggatcg attatacaaa
     1561 cacaaaccag caggattgaa acccacctgg atgagagtct tgaagaaata cttagtttct
     1621 cagtagcctg gctaagaacc acattcaaca gcgatgctgc agagattgta gttcctttca
     1681 atattgatta tccgcagaat gaaataacgg taactgtgcc caaaagtgga gacaaaaaga
     1741 aactgttaga attatcagag aaaaatgtca gtcattttat tgaggagttg aataaaaatg
     1801 acagattaaa acttgatatt aaaaaagctg ataagtttac actacttgtt caattacaga
     1861 aggaccttca actccgggaa ttacccattc atattgaatg cttcgataat tccaactttc
     1921 agggcagcta ccctgtttca gctatggtat gcttcaggaa tggtgaaccc agtaaaaagg
     1981 attacaggcg gtttaatgtg aaaactgtcg aagggatcaa tgattttgcc accatgaaag
     2041 aagccgtttt caggcggtat aagaggcttc tggaagaaga ccagcctttc cctcagctgg
     2101 tgatcatcga cggcggcaaa gggcagttaa acgctgctat ggaagcaata accgaactga
     2161 agttaaatgg aaagtctaca ttggtagggc tggcgaaaaa tgaagaagaa atattcttcg
     2221 ccggagatat tgcgtcactg aaacttccgt ataacagcgg cagccttaag ttcatcagaa
     2281 ggatcag
//
LOCUS       DAORTI010000280         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_73178_length_2271_cov_6.122292, whole genome shotgun sequence.
ACCESSION   DAORTI010000280 DAORTI010000000
VERSION     DAORTI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73178_length_2271_cov_6.122292"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLO70_17280"
     CDS             <1..408
                     /locus_tag="PLO70_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HQZ76275.1"
                     /translation="AAKGWISGSAETYYNDGILNSIKLWLPNWSVPIMTYITAADIAW
                     NNAATPSEKMEKIHLQKYYALFLTDLQQWFEYRRTGHPILPTGPGLQNNGIMPARLTY
                     PVYVQSTNPTNYQAAVAAQGPDVISTQVWWQKP"
     gene            442..1947
                     /locus_tag="PLO70_17285"
     CDS             442..1947
                     /locus_tag="PLO70_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HQZ76276.1"
                     /translation="MKKILSYSFYLIALLSSWGCKKGDNYPGGTVSSYISLFDVRNIY
                     KGTDVTLSLENMFGAEKIAGVVVSDHSGGNLPPGLLILQDNRRLSKLRGITIPIGADA
                     ATYQPGDSLVINVTGALLKKVDGILQLTGINSSDIVKAASGVAINTPIVKSNEVLADP
                     KSFESTLISIAKVGFDPSLPPGSTYAGDRLINDGFGNLTLHTEAGASFANKPLPFLAN
                     YTGIIFNKEGATEPQLWPRVDADITILSATAPKIAAIVVTGYLIDPTGSDANYEYIQL
                     LATKNIDFAATPYSLVTTNNAGANVPTGFPTDGWAMGGTRTYKFNLATGTVLKGQYFY
                     VGANKNIWGAGSTDISTSRWFSKMYSTVSGDDFGSSTTNLLANSGNAAGIAIFDKTAV
                     DVSTVPIDVIFYGGSGSVYTAGPPEKGYRITDTDYYDKVNPSTLADQPYFNMGSNTGK
                     LSFPPATNFVQLGGTYNITSGRWTSARTLNPIVMNASATVALIEGATTIEQ"
     gene            1959..>2271
                     /locus_tag="PLO70_17290"
     CDS             1959..>2271
                     /locus_tag="PLO70_17290"
                     /inference="COORDINATES: protein motif:HMM:NF027689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase N-terminal
                     domain-containing protein"
                     /protein_id="HQZ76277.1"
                     /translation="MKRRKFIEQVSFITGGLLLSHNAAFAIPVGVQKIKGTVLSGGKG
                     IANVIVSDGYSVIATDSKGQYEITPHVDARAVFVSTPSGYEFNHDKCIARHYSLLKKN
                     DE"
BASE COUNT          740 a          388 c          460 g          683 t
ORIGIN      
        1 gcagcaaaag gttggatcag tggatctgca gaaacatatt ataacgatgg tattcttaac
       61 agtataaaat tatggttgcc taactggtca gttcctatta tgacttatat aactgccgca
      121 gatattgcat ggaataatgc agcaacgcca agtgagaaaa tggaaaagat acatctacag
      181 aaatactatg cattatttct tactgatttg caacaatggt ttgaatatcg tagaaccggc
      241 catccgatat taccaactgg tccggggctt caaaataatg gtattatgcc cgcaaggcta
      301 acttaccctg tatatgtaca atctaccaat ccaacgaact atcaggcggc agtagcagca
      361 caaggaccag atgtaatttc aacacaagtt tggtggcaaa aaccataaat aataaaactg
      421 aaccagctta aaatacacac aatgaaaaaa atattatcat attcatttta cctgattgct
      481 ttacttagct cttggggatg taaaaaaggt gataactatc cgggcggaac agtcagttct
      541 tatatttctc ttttcgatgt aagaaatatt tataaaggca ctgatgtaac tctatcttta
      601 gaaaatatgt ttggtgccga aaaaatagca ggagttgtgg tttcagatca ctctggtggt
      661 aatctgccac cagggttgtt gatactacaa gataaccgcc gtctgtctaa gctaagaggt
      721 attactatac ctattggtgc cgatgctgca acttatcaac ctggcgattc tttggttata
      781 aatgtaactg gtgctttgtt aaaaaaagtt gatggaattc tccaattgac aggaattaat
      841 agcagtgata ttgtaaaagc tgcatctggt gttgctatca atacacctat tgtaaaatcg
      901 aatgaagtac ttgcggatcc aaaatctttt gaaagtacat taatttcaat agctaaagtt
      961 ggttttgatc caagccttcc tccgggtagt acttatgcag gagacagact gattaatgat
     1021 ggctttggta accttacgct acatactgaa gcaggagcat catttgcaaa taagccactt
     1081 ccttttcttg ctaactacac aggtataata tttaataagg aaggtgccac ggaaccgcaa
     1141 ttgtggccaa gagttgatgc tgatattacc attctaagtg ctacggctcc taaaattgct
     1201 gcaatagttg ttacaggata tttgattgat ccaacaggaa gcgatgcaaa ttatgaatac
     1261 atacaattgc ttgccacaaa aaatatcgat tttgcagcaa caccatattc tttagttaca
     1321 acaaataatg caggagcaaa tgtaccaact gggtttccta ctgatggatg ggctatgggt
     1381 ggaaccagaa catataaatt taatttagca actggtactg tattaaaggg tcaatatttt
     1441 tatgttggtg ccaataagaa tatatgggga gcaggttcta cagatataag tacttctaga
     1501 tggtttagta aaatgtattc aacagtaagt ggggatgatt ttggaagtag tactacaaac
     1561 cttctggcta atagtggaaa tgctgccggc attgccattt ttgataaaac agcggttgat
     1621 gtaagcacag tgcctataga tgtaattttt tatggtggaa gcggaagtgt ttatacagct
     1681 ggcccacctg aaaaaggata tcgtattaca gatacagatt attatgataa agtcaatcca
     1741 tcaacattgg ccgatcaacc ttattttaac atgggctcta atactgggaa attatctttt
     1801 cctccggcta ctaattttgt acagttaggt ggtacttata atattacttc aggaagatgg
     1861 acgtctgccc gtaccttgaa cccaattgta atgaatgcat cggcaacagt tgctttgata
     1921 gaaggcgcaa caacaattga acaataattt tttataaaat gaagcgaaga aaatttatag
     1981 agcaagtaag ttttattaca ggcgggttgc ttttaagcca taatgctgct tttgcaatac
     2041 ctgtcggtgt tcagaaaata aagggaactg ttttatccgg tggtaaagga atcgcaaatg
     2101 ttattgtaag cgatggctat tcagtaattg ctactgatag taaaggccaa tatgaaatta
     2161 ctcctcatgt cgatgcaagg gcggtttttg taagcacacc atctggttac gaattcaatc
     2221 atgataaatg tattgcccgt cattacagtt tgcttaagaa aaatgacgaa a
//
LOCUS       DAORTI010000281         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_73183_length_2271_cov_4.445848, whole genome shotgun sequence.
ACCESSION   DAORTI010000281 DAORTI010000000
VERSION     DAORTI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73183_length_2271_cov_4.445848"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLO70_17295"
     CDS             complement(<1..455)
                     /locus_tag="PLO70_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQZ76278.1"
                     /translation="MKNPIPHIIADNTFWISPDRCLFWEEQNSLIVADLHLGKTGHFR
                     KSGIAVPQTIYKADLQRLMAQLYLFKVERLIIVGDFTHSIANKELELFLKWRNDFSLL
                     KIDLVKGNHDILADSWYIDANIQIHEWELLIDGFRFRHEDKRIKNDESP"
     gene            complement(482..>2271)
                     /locus_tag="PLO70_17300"
     CDS             complement(482..>2271)
                     /locus_tag="PLO70_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQZ76279.1"
                     /translation="NKKIKVESIFPDEIEKYPWAGHLGIKLAHKLIPIIEASKTTLIF
                     INTRGMSETWYQKLLAIAPELAGAIALHHGSIEPELRHWVEDALHTGTLKAVVCTASL
                     DLGVDFRPVDTVIQVGSPKGVARFLQRAGRSGHTPGDISKIYFLPTHSLELVEAAALK
                     AAIAGQEIESREPLLLCFDVLIQYLCTLAISDGFSKEELYKEIKSTYCFSSMTEEEWQ
                     AILHHLVEGGVALQQYDEYKKVELLNDVYTINNRRIAMRHRMHIGTIVSDAMLKVKFL
                     SGGYIGVIEEYFISRLEPGDVFTLAGRNLELVAIKDMTVSVRNSTAKKSQVPSWLGGR
                     MSLTANLGHRLRKTFNDVLDKTNIPELKILLPLFKLQQGLSHIPKDDELLIEQIEDKD
                     GFHLLVYPFEGRNVHEAMSAILAQRIGKITPITFSISMNDYGFELLSDKPIPVDDTNA
                     GELFSTDNLLMDIQRSINSTEMARRKFRDIAVIGGLIFQGMPGEKKKARHVQSSASLL
                     FNVFSEYDPNNLLLRQAHTEVLLQQMEEVRLRMALQRIQESKIIVTFPAQLTPLSFPI
                     IVDGLNRNNLSSEKLEDRIKKMQQQLEAD"
BASE COUNT          624 a          488 c          435 g          724 t
ORIGIN      
        1 tccggggatt cgtcattttt aatccgcttg tcttcatgtc ggaagcgaaa accatcaatc
       61 agtaattccc attcatgtat ttggatgtta gcatcaatat accagctgtc ggctaaaata
      121 tcatgattcc cttttaccag atcaatcttc aataaagaga aatcgttccg ccattttaaa
      181 aacagttcca gttctttatt ggcgatgcta tgggtaaaat caccaacaat aattaatcgt
      241 tctactttaa aaagataaag ctgtgccatt aaccgctgca agtctgcttt gtaaatggtt
      301 tgaggaacag caattcccga tttgcggaaa tgacccgttt tgccaagatg cagatcggca
      361 acaattaaac tgttttgctc ttcccagaac aggcatcggt ctggtgaaat ccaaaaggta
      421 ttatcagcaa tgatatgggg tattgggttc ttcattcagc agtattcaaa tttagtataa
      481 actagtcagc ctccagttgc tgttgcattt tttttatacg gtcttccagt ttttcagaag
      541 aaaggttatt acggttaagg ccatccacta taattgggaa tgataatggg gtgagttgcg
      601 caggaaaggt tacaattatt ttactttctt gaatacgttg caaggccatg cgtagtcgca
      661 cttcttccat ttgctgaagc agtacttcag tatgtgcttg ccggagtaac aggttgttgg
      721 gatcatactc cgaaaaaaca ttaaagagaa gcgatgctga tgattgcaca tgtcttgctt
      781 ttttcttttc gccgggcatt ccctgaaaaa ttaggccgcc gataaccgca atatctcgaa
      841 attttcgccg ggccatttca gtgctgttaa tacttcgctg tatatccatt agcagattat
      901 ctgtcgaaaa tagttcgccg gcattggtat cgtctacagg tattggtttg tcgcttaata
      961 attcaaaacc ataatcattc atcgaaatgg aaaatgttat aggcgttatt ttaccaatac
     1021 gttgcgccag aattgcactc atggcctcat gtacatttct tccttcaaaa ggataaacga
     1081 gtaaatgaaa accgtcctta tcttctattt gttctataag caattcatca tcttttggta
     1141 tatgcgaaag tccctgctgt agtttaaata aaggcaacag tatctttagc tccggaatat
     1201 ttgtcttatc gagtacatca ttaaatgttt ttcgcaaacg atggccaagg tttgcagtta
     1261 agctcattct tccgcctagc cacgatggaa cctgcgattt ctttgcagtt gaatttcgaa
     1321 cagatacggt catatctttt atagcaacca gctccagatt acggcctgct aatgtaaaca
     1381 catctcccgg ttccagacgg ctgataaaat attcttcaat cactccaata tatcctcccg
     1441 ataaaaattt tactttcaac atcgcatcgc ttacaattgt gccaatatgc attcgatggc
     1501 gcatcgcaat ccggcggttg tttattgtat atacatcatt tagtagttct acttttttgt
     1561 attcatcata ttgttgcaat gcaacaccgc cttctacaag gtggtgcaga atggcctgcc
     1621 actcttcttc ggtcatgcta ctaaagcaat aggttgattt tatttcttta tataattcct
     1681 ctttgctaaa cccgtcggat atagcaagag tacacaagta ttgtatcaac acatcaaaac
     1741 aaagtaacag aggctcccgg ctttcaattt cctgaccggc aatagcagct tttaatgccg
     1801 ctgcttctac cagctccaag gaatgtgttg gtagaaaata tattttgctg atatcgccgg
     1861 gagtatgtcc acttcggccg gctctttgca aaaacctggc tactcccttg ggcgaaccaa
     1921 cttgtataac ggtgtcaact ggtctgaaat caacacccag atcaagactg gcggtgcaaa
     1981 ctactgcctt taatgttccc gtatgcaagg cgtcttcaac ccaatgccgc aattcgggtt
     2041 ctatactgcc atggtgcagg gcaatggcac cggctaattc cggagcaata gctaataatt
     2101 tttgatacca tgtttcactc attcctctgg tattgatgaa gatgagggtg gttttgctag
     2161 cttcaataat cggaattaat ttatgtgcca gttttatacc cagatgcccc gcccagggat
     2221 atttttctat ctcatccgga aagatagatt caaccttaat ttttttattg a
//
LOCUS       DAORTI010000282         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_73975_length_2256_cov_4.365743, whole genome shotgun sequence.
ACCESSION   DAORTI010000282 DAORTI010000000
VERSION     DAORTI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73975_length_2256_cov_4.365743"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLO70_17305"
     CDS             complement(<1..663)
                     /locus_tag="PLO70_17305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ76280.1"
                     /translation="MLIKFTTAFFFLFLGIVSGAQPLSKADKQLLSAFDTLLMSTYKS
                     NETGATAIVSRKGQVIYKKAFGMADLELNVAMQPDMIFRIGSISKQFTGVAILQLAEK
                     GKLSLQDDIKKFIPDYPTHGYTITVEHLLTHTSGIKSYTGMKEFETIMRKDMKPMEII
                     DVFKNQPMEFAPGTKWNYNNSGYILLGYIIEKVSGKTYEEYVEENLFAPAGMSNSGYG
                     NDL"
     gene            complement(770..1948)
                     /locus_tag="PLO70_17310"
     CDS             complement(770..1948)
                     /locus_tag="PLO70_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQZ76281.1"
                     /translation="MSATIKLLKESVDINSGTYNIEGVRKTGELYGKELAALGFTVLW
                     VSLPDSLKRAGHLVAYRVGKKGKKLFLIGHLDTVFEPDMAANPFTMLNDSTATGQGVN
                     DMKGGDVMVIAALKALYQLNLLEDVSITVYFTGDEENAGYPREVSRADFIERAKKHEI
                     ALGFEGAAGLNTVATARRGASEWRLEVEGKQAHSSGVFGAAGYGSIFEAARIINEFRE
                     KLGKEEYLTFNPGLFIGGSEINYDTASQAGVASGKSNIISPKTVVIGDLRFLTEEQKE
                     KARKNMKQIVSQSLPGAKARISFKDGIPSMPPTTGNDALVKIVNDVSLSIGYGTVKAG
                     YPGSRGAGDISYVAKYLDCLDGLGASGKGAHAPGEVINLYEYPRLIKRAALVIYRLTR
                     "
BASE COUNT          623 a          522 c          421 g          690 t
ORIGIN      
        1 taaatcattc ccatagccag agttcgacat accggctggt gcaaataaat tttcctctac
       61 atattcttcg tatgtttttc cggatacttt ttcaataata taacccaaga ggatataacc
      121 agaattatta tagttccatt tggtgcccgg ggcaaattcc atgggttgat ttttaaacac
      181 atcaataatt tccatcggct tcatatcctt tctcattatg gtctcaaact ctttcatacc
      241 ggtataactt tttatcccgg atgtatgtgt caacaggtgc tccaccgtta tagtatatcc
      301 atgcgttgga taatcaggaa taaatttttt aatgtcgtcc tgcagggaca actttccttt
      361 ttctgccagt tgcaatatag ccaccccggt gaattgctta ctgatggagc caatacggaa
      421 gatcatatcg ggctgcatgg ccacattcaa ttccaaatca gccataccaa aagctttctt
      481 atagatcacc tggcctttgc gactaacaat agctgttgcc ccggtttcat tactcttgta
      541 tgtagacatc aataaagtat caaatgctga gagcagttgt ttatctgctt tggaaagtgg
      601 ttgtgcgcca gaaacaatac cgagaaataa aaaaaagaat gcagttgtga attttataag
      661 catggtttga tggtttgatt tgaatgggaa agttacactt tccaggaata ttttcagctg
      721 gccaaaacgc agcattccag ttttacaaaa aaagagacga aaaaatattt tatcgggtga
      781 gccggtatat aaccaatgca gctcttttga tcaacctggg gtattcatac aggtttatca
      841 cttctccggg agcatgagct ccttttcctg aagcaccgag accatccagg caatctaggt
      901 attttgctac ataagatata tcaccagctc cccgggaacc gggataacct gcttttacag
      961 taccataccc aattgagagg ctaacatcat tcactatttt caccaatgca tcattgccgg
     1021 ttgttggtgg catagaagga ataccatctt tgaaactgat cctggctttg gcacccggca
     1081 agctctgtga tacgatttgc ttcatatttt ttctggcttt ctctttttgt tcttcggtga
     1141 gaaatcgcag atcaccaata accactgttt ttggtgaaat gatattcgat ttacctgaag
     1201 ccacaccagc ctgtgatgca gtatcataat ttatttcaga cccgccgatg aacagaccgg
     1261 gattaaaagt gagatactct tctttaccta atttttccct gaattcatta atgatcctgg
     1321 ctgcttcaaa gattgaacca taccccgcag caccaaagac cccggatgaa tgtgcctgtt
     1381 taccctctac ttccagtctc cattcactgg caccacggcg ggccgtagcc acagtattca
     1441 aaccggcagc accttcgaat cctaaagcaa tctcatgctt ctttgctctt tcaataaaat
     1501 cagcacggct tacttccctt ggataaccgg cattttcttc atccccggta aaataaacag
     1561 ttatagatac atcttccagc aggtttagct ggtacaatgc tttcaatgcc gcaatcacca
     1621 ttacatcacc tcccttcata tcatttacac cctggcctgt tgcagtactg tcattcagca
     1681 tagtgaaggg atttgcagcc atgtcgggct caaacactgt atccagatgg ccgatcaaaa
     1741 aaagcttctt cccttttttc ccaacccggt atgccaccag gtgccctgcc ctttttaatg
     1801 agtcgggcaa ggatacccat aatactgtaa agcccagtgc agctaattct tttccatata
     1861 actcgcctgt tttccgtaca ccctctatat tgtaggtgcc gctgtttata tccaccgact
     1921 ctttcagcaa cttaatcgtt gcggacatat ccgaatcgat acttttcaga acctgatgtt
     1981 ccgttgcaga aagagattgg gtatgggctg caaaaccaat tacttataac agggcagcac
     2041 aaaaatattt ttttatcata ctttattggc aagaaggcgt aaataatata tttccgaaat
     2101 tagtaaatca tcccaggaat tgttgctccg tacaacatta tcctcgttaa gacgtatata
     2161 gagtctatac cgggcgagtt ggaagagagt gcatttattg atggtgcatc atatatggta
     2221 gtatttcgca agttgatttg gcctttgagt aagccg
//
LOCUS       DAORTI010000283         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_77645_length_2190_cov_6.417330, whole genome shotgun sequence.
ACCESSION   DAORTI010000283 DAORTI010000000
VERSION     DAORTI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77645_length_2190_cov_6.417330"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..792)
                     /locus_tag="PLO70_17315"
     CDS             complement(73..792)
                     /locus_tag="PLO70_17315"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQZ76282.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPDVVKAAIDAQRFGADGVTVHP
                     RPDERHIRYDDVREIKKIIVTEFNIEGNCTEQKFVDLVLETKPDQVTLVPDALGQLTS
                     DHGWDTIKNKNYLQDIIGVFKKAGIRVSIFVDPDIKIVEGAAATGTDRIELYTEGYAK
                     QYAAGNKQAAVKSYVEAAVKAKELGLGLNAGHDLDLQNLQYFKQQIPWLDEVSIGHAL
                     ICDALYLGFENAIQLYKRQLQ"
     gene            893..1495
                     /locus_tag="PLO70_17320"
     CDS             893..1495
                     /locus_tag="PLO70_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76283.1"
                     /translation="MKKICLCGAGLFMLYALSAQPFNLDTAINPVELKLLPFKPTAKE
                     RELEKGRMNITRVTQSDDTLYYFVQGASIYSPVYVGVTSLDPANKIDVRLHKMNWKNA
                     NRSGSTDEKGQWSEKFKTENDFGIMVIPHSKPAKYTLLVWVGDEAKIELPPVLDKDKD
                     AASGGGKGGGMSQSTILYIVIGALAVIVAFLFFKLKNRKS"
     gene            1492..2184
                     /locus_tag="PLO70_17325"
     CDS             1492..2184
                     /locus_tag="PLO70_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76284.1"
                     /translation="MRYIRQILLPVLFLLVSAGAVKAQAGANEIDQATEQTMQLLEQL
                     FGNMEGFEEGVGPAFEAFRNGMGIVRDGLEFYNSMQALDENECVPDFTTPAEAMMPTG
                     CADNEECASCYDESINRLNTVRKSLARMRCIYTNTKKFTDAAISFGDNVSGIHGVMGI
                     AWQNERRGIEKSLQDFEKTYDKKYADLMGSMQTALNGINACEAKFGMRDWYQRFGFLY
                     FEMMKEKYKRDD"
BASE COUNT          604 a          443 c          521 g          622 t
ORIGIN      
        1 cactaacact taaatatacg caactgctgg catagtttta cattgatact caacgagaat
       61 ttatgccagt atttattgca gttgtctttt gtacaactgt attgcattct caaatcccaa
      121 atacaaagca tcacagatca atgcatggcc aatactcact tcatccagcc agggtatctg
      181 ttgtttaaaa tattgcaagt tctgcaggtc aagatcatgg ccggcattta aacccaggcc
      241 cagttctttt gcttttacag cagcttccac atacgatttt acggctgcct gcttattacc
      301 tgctgcatat tgttttgcat aaccttccgt gtacagttca atccggtcgg taccggtggc
      361 tgcagctcct tccacgattt ttatatccgg gtctacaaat atgctgacac ggataccggc
      421 ctttttaaat acgccaatga tatcctgcag gtaatttttg tttttgatgg tatcccagcc
      481 atggtcgctg gttagctggc ccagtgcatc gggtaccagg gttacctggt cgggttttgt
      541 ttccagtacc aggtccacaa atttttgttc ggtgcagttg ccttctatat taaattcagt
      601 aacgataatt tttttaatct ctctcacatc atcataacgg atatgccgtt catccggcct
      661 tggatgtact gtaacgccat ctgcaccgaa acgctgggca tcaatggcgg ctttcactac
      721 atcaggatta ttgccgcctc tggagttccg cagggtggcg aatttgttga tattaacaga
      781 cagtttggtc atacaacaaa tttacaggta aaataagaaa agccgcctcc tgccagccgg
      841 gagtagtatt tcttaaaatg atttattaca tttaagaaac taaatttttc ccatgaaaaa
      901 aatctgcttg tgtggggcag ggttgtttat gctatacgct ttgtcagctc agcccttcaa
      961 cctggacact gctatcaacc cggtagaact gaaactgctt ccgttcaaac caacagccaa
     1021 agaaagggaa cttgaaaaag gccggatgaa tattacccgt gtcacccagt cggatgatac
     1081 gttgtattat tttgtccagg gggccagtat ctattccccg gtatatgtgg gagtcacttc
     1141 tctcgatccg gccaataaga tcgatgtgcg gctacataaa atgaactgga aaaatgctaa
     1201 ccgcagcggc agcactgatg agaaaggtca atggagtgaa aaattcaaaa cggagaatga
     1261 ttttggtatc atggtcatac cacactccaa accggcgaaa tataccttac tggtttgggt
     1321 gggtgatgaa gccaaaattg aattaccccc ggtactggac aaggataagg atgccgcaag
     1381 cggtggtgga aaagggggtg gcatgtcgca gtctaccata ctgtacatag tgattggtgc
     1441 attggctgtt attgttgcat ttttattctt taaacttaaa aatcgtaagt catgagatat
     1501 atcaggcaaa ttttattacc ggttcttttt ctgttggtat cagccggtgc tgtaaaagcc
     1561 caagcgggtg ctaacgaaat tgaccaggct acggaacaaa ccatgcaatt gctggagcaa
     1621 ttatttggta acatggaagg ttttgaagaa ggagtagggc ctgcttttga agctttccgg
     1681 aatggtatgg gaatagtcag ggatgggttg gaattctata attccatgca ggcattggat
     1741 gagaatgaat gtgtgcctga ttttacaacc cctgctgagg cgatgatgcc gacgggctgt
     1801 gcggataatg aggaatgtgc aagctgctat gatgagtcta tcaaccggct gaatacggtc
     1861 cggaaaagcc tggcccggat gcgttgtatt tataccaaca ctaaaaagtt cacggatgcg
     1921 gctatctctt tcggggataa tgtatccggc attcatggtg tgatgggtat agcatggcag
     1981 aatgaaagaa ggggtattga aaaatcattg caggattttg aaaaaaccta tgataagaaa
     2041 tacgcagacc tgatgggctc catgcaaacg gctttgaatg gcatcaatgc ctgtgaagca
     2101 aaattcggaa tgcgggactg gtaccagcgg ttcgggtttc tgtattttga aatgatgaaa
     2161 gagaagtata aacgggatga ttaagagggg
//
LOCUS       DAORTI010000284         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_82951_length_2106_cov_5.482691, whole genome shotgun sequence.
ACCESSION   DAORTI010000284 DAORTI010000000
VERSION     DAORTI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82951_length_2106_cov_5.482691"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLO70_17330"
     CDS             complement(<1..644)
                     /locus_tag="PLO70_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQZ76285.1"
                     /translation="MKKSVIIVQLLLAFVYTGAQSTAGITGTRDTSYNITNEYNKHLK
                     NYPNLQIVKEFSYPYITEEKNISYCKTNERELKLDVFSPSEKSTTKRTAILFIHGGGW
                     RSGNKAMHYPLLQQLASLGYVCITPEYRLSTEALYPAAVHDIKSAIRWVRKNSAKYNI
                     DVNKIVVSGHSAGGELAAFMGATNGMKQFEGDGCYKNISSKVNAVIDLDGTLAF"
     gene            complement(645..1400)
                     /locus_tag="PLO70_17335"
     CDS             complement(645..1400)
                     /locus_tag="PLO70_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HQZ76286.1"
                     /translation="MKKIILVPLAMVLLAFIMPAKKKIKIFLAGDSTIAIKETKAFPE
                     TGWGMPFVHFWDSTVTVVNKAKNGRSTKTFLSEGLWKSIMDEAKEGDYVFIQFGHNDE
                     SVEKKERYSTPDTFKMNLSRFIKETRDKKATPVLFTPVSRRKFDKEGLAVETHKAYSA
                     LTREVALEQNVLFIDLDEKSRSLYQQFGPEHSKLLFLQLQPGEHPNYPDGRNDNTHFN
                     ELGARLIAQLVLKELKGLNTELNNRIIIPAVKK"
     gene            complement(1397..>2106)
                     /locus_tag="PLO70_17340"
     CDS             complement(1397..>2106)
                     /locus_tag="PLO70_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQZ76287.1"
                     /translation="PRFNGTRLGAEQEFVDYSNNVIYNWGGNNVYGGEAGNYNIINNY
                     YKYGPSTNKNVRFRIVNPSRTDKLGFGKFFVDGNHVEDAKDISMNNRLGIDMGNGGTE
                     ADKKESVQDKAFIAEPITIQSAKEAYEDVIQFAGASFRRDTLDQRIINDVKNRTGKFI
                     DVQGGYPHGTAYELTVNAWPALQSLLALTDTDKDGMPDEWEKKNGLNPGDATDATQSK
                     LHPYYNNIEVYINSLIK"
BASE COUNT          532 a          446 c          399 g          729 t
ORIGIN      
        1 ataaaagcca gtgtgccgtc cagatcaatt actgcattga ctttgctgga tatattttta
       61 taacagccat ctccttcaaa ctgcttcatt ccgtttgttg cacccataaa agcagccagt
      121 tcgccaccgg cagaatgacc ggatactact atcttgttta catcaatatt atattttgct
      181 gaattctttc tcacccagcg gatagccgat ttaatatcat gaacggctgc cgggtacaaa
      241 gcctctgtgg aaagacggta ttcgggagta atacaaacat aaccaagtga agctaactgt
      301 tgcaacaaag ggtagtgcat ggctttgttg ccgcttctcc aaccgcctcc atgaataaaa
      361 aggatggctg ttcttttggt tgttgatttt tcacttgggg aaaatacatc cagctttaat
      421 tcccgttcat tggtcttaca ataactgata tttttttctt ccgttatata tgggtatgaa
      481 aattccttta caatttgcag gttaggataa ttcttcaggt gtttgttata ctcattggta
      541 atattatacg aagtgtccct tgttcctgtg ataccggcag tggattgtgc cccggtgtat
      601 acaaaggcaa gcagtaattg tactatgatg acactttttt tcatttactt ttttacagca
      661 gggatgatga tcctgttgtt tagttcagta ttcagccctt ttaactcttt taatactaac
      721 tgggcaatca atcttgcccc cagctcatta aaatgtgtgt tatcgtttct tccgtcagga
      781 taattaggat gttctcccgg ctggagttgc aggaataata atttggaatg ttccgggcca
      841 aattgctggt acagtgacct gctcttttca tcgaggtcaa tgaataaaac attttgttcg
      901 agtgctactt ccctggtcaa tgccgaatat gctttgtgtg tttcaacggc caacccttcc
      961 ttgtcaaatt ttcttctgga gaccggagtg aataatacag gggttgcttt tttatccctt
     1021 gtttctttaa taaacctgct gaggttcatt ttaaacgtat ccggtgtgga gtatcgttct
     1081 ttcttttcaa cggattcatc gttatgtcca aactggataa aaacatagtc accttctttt
     1141 gcttcatcca taatggattt ccataaacct tcgctcagaa atgttttggt gctgcggcca
     1201 ttttttgctt tgttcacaac agtaacagta gaatcccaga aatgtacaaa gggcatgccc
     1261 cagccggttt cagggaaggc ctttgtttcc ttgatggcaa tagtagaatc tccggccagg
     1321 aagattttta ttttcttttt ggcaggcatt ataaaagcta acaataccat agctaatggt
     1381 accagtataa tctttttcat tttatcaggc tatttatata cacttctata ttgttatagt
     1441 aggggtgaag tttgctctgg gtggcatcag tagcatcacc gggatttaag ccattctttt
     1501 tttcccattc gtccggcatg ccatctttat ctgtatctgt tagtgccagt aatgattgta
     1561 atgcaggcca tgcatttaca gttagttcat aagctgttcc atgcgggtag ccaccctgca
     1621 catctatgaa ttttccggtt ctgttcttca catcattgat gattcgttga tccagggtat
     1681 ctcttctgaa actggccccg gcaaattgta tcacatcctc gtaggcttct ttagcagatt
     1741 gaattgtgat gggttctgca ataaaagctt tgtcctgaac cgattctttt ttatcagcct
     1801 cagttccgcc attacccata tctataccca gcctgttatt catggaaata tctttagcat
     1861 cctcaacatg gttaccatcg acaaagaact ttccaaagcc caatttatct gttctggacg
     1921 ggtttacaat tctgaaccgt acattcttgt tggtggaagg accatattta taatagttat
     1981 tgatgatatt ataattcccg gcttcacctc catatacatt attaccaccc cagttataaa
     2041 ttacattgtt actgtaatca acaaattcct gttctgctcc caaacgggtg ccattgaatc
     2101 ttggat
//
LOCUS       DAORTI010000285         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_85566_length_2066_cov_5.777225, whole genome shotgun sequence.
ACCESSION   DAORTI010000285 DAORTI010000000
VERSION     DAORTI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85566_length_2066_cov_5.777225"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..619)
                     /locus_tag="PLO70_17345"
     CDS             complement(239..619)
                     /locus_tag="PLO70_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQZ76288.1"
                     /translation="MNVKTDTKEKFHAITVSDPILSATMTDGIGECLLPYLQNDVKNL
                     VLILKNVTSIDTVAAEKLVYIQQKFYENSASFVICELQKQVEDFLDKNELLEMMNVTP
                     TESEAWDIVQMEEIERDLMDGENF"
     gene            784..>2066
                     /locus_tag="PLO70_17350"
     CDS             784..>2066
                     /locus_tag="PLO70_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQZ76289.1"
                     /translation="MDNNFSAQVKEIISFSREEALRLGNDFIGTEHLLLGLIREGDNT
                     AVRILKSFNVDLYELRKEIELAVKDKTGKNIANINSLPLTKQAEKVIRVTVLEAKALK
                     SATVETEHLMLSILKNKENIATQILNQFDVDYDLFRQELGIVKSNDPRAEFQDENDDD
                     FDEEKKYSSPKGGAKQQGAAKSKTPVLDNFGRDITKMAELGALDPIVGRESEIERVSQ
                     ILSRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRVLFDKRVISLDLAALVAGTK
                     YRGQFEERMKAIMNELEKNRDVILFIDELHTIVGAGGASGSLDASNIFKPALARGELQ
                     CIGASTLDEYRMYIEKDGALDRRFQKVMVEPPSVDDTIQILNNIKSKYEDYHNVNYDQ
                     EAIDACVKLSDRYVTDRLLPDKAIDVLDEVGARVH"
BASE COUNT          640 a          391 c          458 g          577 t
ORIGIN      
        1 cttataattt atcagctgta tctgggttcc ttcaccgcaa tcaaccaggt aattactgcc
       61 atccagcgtc accacctggc tggtaggatg acggtcaaat gcaggaactg ccgaattatt
      121 accaaggatg gtgactgcaa acatgaatat aacagcggtt tttgatgtgt ttggtagtac
      181 taaagaccgg agctttaact aattagctgc atataatccg gcaaacatat tttctggttc
      241 agaaattttc cccatccatc aaatctctct ctatttcttc catttgaaca atgtcccagg
      301 cctcactttc agtaggggtt acgttcatca tttctaacaa ttcatttttg tcaagaaaat
      361 cctccacttg tttttgaagt tcacaaatta caaaagaggc gctgttttcg tagaatttct
      421 gttgtatgta aacgagtttt tcagcggcaa ccgtatcaat ggatgttacg ttttttaata
      481 tcaaaaccag gtttttaacg tcattttgca ggtaaggaag taaacattct ccaatgccat
      541 ctgtcatagt agcagataga atcgggtctg aaactgtgat ggcatgaaat ttctctttgg
      601 tatcagtttt gacattcatt agttattact ttttttatta ttgcgtttgt ccggcagcgg
      661 acctgcaaag tgaaaaagtc gtggttttcc tacaacttaa attatcaaca ttccgtttat
      721 aagtacagga gaacaagaac caaatttatt cgttaatatt gtataacaac caattatcta
      781 aaaatggata ataatttttc agctcaggtt aaagaaataa tctcattcag cagggaagag
      841 gcattaaggc ttggaaacga ttttatcggc actgagcatc ttttgctggg tcttatccgc
      901 gaaggagata atactgccgt acgtattctg aagagcttca atgtggacct ctatgaatta
      961 cggaaagaaa ttgagctggc cgtaaaggat aagacaggga aaaatatcgc caatatcaac
     1021 agcctgccac tgaccaaaca ggcagaaaaa gtaataagag taacggtatt ggaggcaaag
     1081 gctttgaaaa gtgccactgt ggaaacagag cacctgatgc tttccattct gaagaacaag
     1141 gaaaatattg ctacacaaat cttaaatcaa tttgacgtgg actacgatct tttcaggcaa
     1201 gagctgggca tcgtaaaatc taacgatccc cgtgccgaat tccaggacga gaatgatgac
     1261 gattttgatg aagaaaaaaa atattcatcc cccaaaggcg gtgcaaagca acagggtgcc
     1321 gcaaaaagca agacccctgt acttgacaac tttggccgtg atataaccaa gatggctgaa
     1381 ctcggagctt tggatcctat tgttggccgt gagtctgaga ttgaaagagt gtcgcagata
     1441 ctttcccgtc gtaaaaagaa caaccccatt ttaattggtg agccgggtgt gggtaaaaca
     1501 gccattgtgg aagggctggc ccttcggatc gttcagcgta aggtatcaag agtattgttt
     1561 gataaaagag tgatctcact cgacctcgct gcactggtag caggtacaaa atatcgtggc
     1621 cagtttgaag aaaggatgaa agccatcatg aacgaactgg aaaagaaccg ggatgttatt
     1681 ttatttatcg atgagttgca cactattgta ggtgctggtg gagccagtgg ttcattggat
     1741 gccagtaata tattcaaacc cgcattagca aggggagaac tccaatgcat aggtgcatct
     1801 acactggatg aatacaggat gtatattgaa aaagatggcg cactggaccg tcgtttccaa
     1861 aaggtaatgg ttgagcctcc aagtgtagac gataccatac aaatactgaa caatatcaaa
     1921 tcgaaatatg aggattatca caatgtgaat tatgaccagg aggccattga tgcttgtgtg
     1981 aaactcagtg acaggtatgt tacagacagg ttgcttcccg acaaggccat tgatgttttg
     2041 gatgaagtag gcgcaagggt gcattt
//
LOCUS       DAORTI010000286         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_86274_length_2056_cov_6.691654, whole genome shotgun sequence.
ACCESSION   DAORTI010000286 DAORTI010000000
VERSION     DAORTI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86274_length_2056_cov_6.691654"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1701
                     /locus_tag="PLO70_17355"
     CDS             16..1701
                     /locus_tag="PLO70_17355"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQZ76290.1"
                     /translation="MKRIISILFTLAITIQAGSQQVYYNNPILAGFYPDPSICRADED
                     YYLTTSTFAYFPGLPIFHSKDLVNWKQVGHAINRAEQMNFAGAGVSRGLFAPSITYHK
                     GTFYIVCTLIDKLGNFVITAKDPKGPWSNPVALPQVNGIDPSLFFDEDDKAYIIYNSI
                     PPDNKPMHDGHRTIRMYSLDWQNLKVTGSETLLVNGGTDMAKKPVWIEAPHIFKKDGW
                     YYLICAEGGTGYNHSEVVFRSKRVDGPYIPYEKNPILTQRHLDKNRPNPVTTAGHADF
                     VQTIDGNWQAVFLACRPYEANYFNTGRETFMLPVTWKDGWPHILEGDETIQFKNPVPL
                     PAITKRVNNPLSGNFVYKDGFKNKELDHRWIFLRAPLENWYNLASKKGYLSVRLRPET
                     VAERQNPSFVGHRQQHLKGQAITSLSFTATSENEKAGLIIFQNEQHHYFICQSSKNNE
                     QVIELFKGPGNANAGKEAELLASQSLKGKKKSIQLKITFDNADYAFWYSTGGNNWTLL
                     KDKVDGRFLSTATAGGFVGNVFGLYATSTGKESNNTAYFDWFEYKGEDDVYKQ"
     gene            complement(1735..>2056)
                     /gene="ssrA"
                     /locus_tag="PLO70_17360"
     tmRNA           complement(1735..>2056)
                     /gene="ssrA"
                     /locus_tag="PLO70_17360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          655 a          420 c          431 g          550 t
ORIGIN      
        1 ataaatgcat aaacaatgaa aagaattatc agtatcctgt ttacccttgc aataacgata
       61 caggcaggct cacaacaagt ttattataac aacccgatac tggccgggtt ctatcccgat
      121 cccagtattt gtcgtgccga tgaggattat tatctcacca cttccacatt tgcttatttc
      181 ccggggcttc cgatttttca cagcaaagac ctggtgaact ggaaacaggt cgggcatgca
      241 ataaacaggg ctgaacaaat gaattttgcc ggtgccggtg tatcaagagg attatttgca
      301 ccttctatca cttatcataa aggaacattc tatattgtct gtactcttat tgacaaactg
      361 ggcaactttg tgatcacggc aaaagacccc aaaggaccat ggagcaatcc tgttgcattg
      421 ccacaggtga atgggattga cccttcgttg ttttttgatg aagacgataa agcatatatt
      481 atttataata gcatcccgcc tgataacaaa cctatgcatg atggtcaccg caccatccgt
      541 atgtattcat tagactggca gaacctgaaa gtcacaggca gtgaaacatt attggtgaat
      601 ggtggtacag atatggcaaa aaaaccagtc tggatcgaag cgccgcatat ttttaaaaaa
      661 gacggatggt actacctgat ctgtgcagaa ggtggtacgg gatataatca ttccgaagta
      721 gtattcagga gtaaaagagt ggacgggcca tatatacctt atgaaaagaa ccctatctta
      781 acgcaacgtc atttagacaa aaaccgcccc aaccctgtaa ccactgccgg tcatgcggac
      841 tttgtacaaa ctattgatgg caactggcaa gccgtttttt tagcctgccg tccttacgaa
      901 gctaattact ttaatacagg cagggaaacg tttatgttac cagtaacctg gaaagacggt
      961 tggccacata tcctggaagg agatgaaaca attcaattca aaaatccggt tccactacct
     1021 gcaatcacaa aaagagtaaa caatccattg tcgggcaatt ttgtttataa agatggtttc
     1081 aaaaataagg aactcgatca tcgctggata tttttaagag cacccctgga gaactggtat
     1141 aacctggctt cgaaaaaagg atacctgtct gtcaggctga gacctgaaac agttgcagaa
     1201 agacaaaacc catcttttgt cggacaccgt cagcaacacc tgaagggaca agccatcact
     1261 tctttatctt ttactgccac tagtgaaaac gaaaaagcag gactgatcat tttccagaat
     1321 gaacagcatc attatttcat ctgccaatca agtaaaaaca atgagcaggt gattgaactc
     1381 ttcaaaggac cgggaaatgc aaatgcaggc aaagaagcgg aactgctggc ttcacaatca
     1441 ttaaaaggaa agaaaaaatc aattcaattg aaaattactt ttgacaatgc agattatgca
     1501 ttctggtatt caacaggagg taataactgg acattactga aagataaagt ggacggcaga
     1561 ttcttaagta cagctacagc cggggggttt gttggcaatg tctttggatt gtatgctact
     1621 tcaactggaa aagaaagtaa caacacagct tattttgatt ggtttgaata taaaggagaa
     1681 gatgacgttt acaaacaata aggcccgcaa tattacattg caggtcttat tgtttagtgg
     1741 aggcgaccgg aatcgaaccg gtgtccaaac atattcttca aaagctttct acatgtttat
     1801 tttgttttgt tatgtcggga atcaactggg aacaaacaac cgattaattc cttagctgta
     1861 tggtcttaag caatcagcac agcctttgat tgcagcaacc tgttttgttt ttaagtcggc
     1921 ggcggggcat ggtaacaagt caaccggccc ggcggcccta atgactactt aatcactgat
     1981 taggcagcca tggcatactg agtattgcca tttatagttt tcgtattcag attaaactgc
     2041 taattacgca acgcag
//
LOCUS       DAORTI010000287         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_87070_length_2045_cov_5.490955, whole genome shotgun sequence.
ACCESSION   DAORTI010000287 DAORTI010000000
VERSION     DAORTI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87070_length_2045_cov_5.490955"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PLO70_17365"
     CDS             <1..1225
                     /locus_tag="PLO70_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQZ76291.1"
                     /translation="LTTPDLEYDMSTDIGIYKNGGKVVNKTTTLTSKEGYYYAGLRDI
                     YFKNNVELKDPAYFIKTDSLLYNTQSQTTRFIAETFIKDSSGRTIETKEGFYNMATGK
                     AEFGQRPVIKDGKTTIIADKVAFNDSLGTSQAIGNVVIVDTAQGTTILAGEVYRDNKR
                     DRMLATRKPLMIIKQDNDSIYVTADTLFSARLSDLYGTKDSLVKDTIKGTKVVNVNKK
                     DSTDRNFEAYSHVRIFADSMQAVCDSLFYSFKDSAFQLYKDPVLWSKESQITGDTVFI
                     FTKNKKADRVKVYENSFLVNKTDPEVYNQVKSIRMDAYFKDGSLDSVRASGYTECIYY
                     IQDEDSAYTGINETKSDVMDIYFGEQALQKVVFRSSVTGTIWPIKQKSPSEMRLQNFR
                     WLEDRRPKNKYELFE"
     gene            1415..1903
                     /locus_tag="PLO70_17370"
     CDS             1415..1903
                     /locus_tag="PLO70_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76292.1"
                     /translation="MKKIILSVIVITTLSLANTVAAQNYKTALGVRLSSSNAMVNNAV
                     SIKHFINERTAIEGLLSFGDPLAIGALVEFHKPLAAEGLTYFYGAGGYLAFLKTVNTT
                     TQKTSTDPNIGAQGVIGLDYKFANIPLNLSLDWKPELNIVTDINFEPAAIGFTARFTF
                     GK"
BASE COUNT          662 a          414 c          398 g          571 t
ORIGIN      
        1 ccttactacc cccgatcttg aatatgatat gtcgactgat atcggtattt acaagaacgg
       61 cggtaaggtg gttaataaaa caaccacgct gaccagcaaa gaaggatatt attatgccgg
      121 attgagagat atctatttta aaaacaatgt ggaattaaaa gacccggctt attttattaa
      181 aacagattca ttactttata atacacaaag ccagaccacc cggttcattg cggagacctt
      241 tatcaaagac agcagcggca gaacgattga aacaaaagaa ggcttttata atatggcaac
      301 cggcaaagcc gagtttggtc aacgacctgt catcaaagat ggaaaaacaa ctatcatagc
      361 tgataaggtc gccttcaatg acagcctggg tacctcacag gccatcggta atgtggtaat
      421 tgttgatacg gcacagggca ctactatctt agctggtgaa gtataccggg ataataaacg
      481 agaccgcatg ctggccacca ggaagccact aatgatcatt aagcaggaca acgattcgat
      541 ctatgttaca gcagatactt tattctctgc ccgtctcagt gatctttatg gcacaaaaga
      601 ttccctcgta aaagacacga tcaaaggaac aaaagtggtc aatgtaaata agaaagacag
      661 tacagaccga aattttgaag cctatagtca tgtccgcatt tttgccgatt ccatgcaggc
      721 ggtatgcgac agtttatttt attcttttaa agactcggct ttccagcttt acaaggaccc
      781 ggtactatgg tcaaaagaaa gccagatcac aggtgacacc gtatttattt ttacaaaaaa
      841 taaaaaagcc gaccgggtaa aagtatatga gaacagtttc ctggttaata aaactgaccc
      901 tgaagtttac aaccaggtaa aatccatccg catggatgct tatttcaaag atggttctct
      961 tgattctgta cgggcatccg gatataccga atgtatctat tatatacagg atgaagacag
     1021 tgcctacacc ggcatcaatg agaccaaaag tgatgtaatg gatatttatt tcggggagca
     1081 ggctttacaa aaagttgtat tccgcagtag tgttaccggc actatctggc ctataaaaca
     1141 aaaatcacct tctgaaatgc gtttgcaaaa cttccgctgg ctggaagatc gccggcctaa
     1201 aaacaaatat gagttgtttg aataattttt actgaaaaaa tacatcctga ccttattgaa
     1261 atgtgataaa ctgcatccgg caagcttata cctgctgttt cattgtaaaa tgctgttaat
     1321 tcaaaaaggc atcaattatc ctccttcaac ttttgcattt tctttagact ttgatggcat
     1381 cattttcgtt tagaatgaag caaaacaaat catcatgaag aaaataattc tttccgttat
     1441 tgttattacc actctgagtt tggcgaacac agtcgctgca caaaattata aaacagcatt
     1501 gggtgtccgg ctcagcagct ccaatgctat ggtcaataat gctgtttcaa taaaacattt
     1561 catcaatgaa agaacagcca ttgaaggcct gttatcattt ggagatccgt tagcgattgg
     1621 tgcgctggta gaatttcata aaccacttgc ggcagaagga ttaacttatt tctatggagc
     1681 cggcggttac ctggcattcc tgaaaacagt aaatactact acccaaaaaa catcaaccga
     1741 ccccaatatt ggtgcacagg gtgtaattgg tcttgactat aagtttgcga acattccgct
     1801 aaacttatcg ttggactgga aacctgaact gaatatcgta accgatatca actttgaacc
     1861 tgctgcgatt ggttttactg cgaggttcac ttttggaaaa taattttctt cgctacaaaa
     1921 aatatataat tagcaggaag ctgtttcaaa aacaaacaaa tttgtcacat tgtcccgata
     1981 gctatcggga tatcgaaatg cgaacaaatt tgtttcccaa tcattgccct atttcggctt
     2041 tgctc
//
LOCUS       DAORTI010000288         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_87901_length_2033_cov_10.859960, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000288 DAORTI010000000
VERSION     DAORTI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87901_length_2033_cov_10.859960"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1187)
                     /locus_tag="PLO70_17375"
     CDS             complement(87..1187)
                     /locus_tag="PLO70_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQZ76293.1"
                     /translation="MDPQLIIEKYQNAIIQIATATGTGTGFYIKEFDLIVTNDHVVAE
                     NAEVTIAGKTFDKALSRVWYTDRKHDLAFLEAPKNIELPEVRLGQYDQMKDGDAVVAI
                     GHPYGLNYTATQGVISKVDRIRDGLKFIQIDAAINPGNSGGPLVNMNGEIIGVNSFII
                     RGGDNLGFALPVLYLREALQMYLPNKGQASARCFSCGYLVTAANIDSGKYCPSCGTEV
                     KLPEIPEKEAEPVGAAKTIEEILKELGKDVRLAREGVNTWSVKEGSAKIKITYNTENF
                     FIAGDAYLCQMPADVSKIKPLYQYLLQENYRTHGLVLSCVKQNIVLSRIIYDLDMTKE
                     NGAEAFRILFKKADHYDDYLKHEFGCSARLEE"
     gene            complement(1206..>2033)
                     /locus_tag="PLO70_17380"
     CDS             complement(1206..>2033)
                     /locus_tag="PLO70_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76294.1"
                     /translation="DKQDDLLVQDFTALEKSDNEHISVVPEQEKEVKYMYLQKWIGET
                     LDTIAPLDADKFSGAIAYLLLVLIHRIDYMITPEGKLLNELEKITGIYFKKDERPVTE
                     KNRDMAEAFKNMQALPKEEVFKNLFRSKYTFAIVAPQPYKAVAEAIHAANQNMVWYRD
                     NNYPFIASQITEYGISYCQYSYSLPKAVTELFHLYMMVNYPEYFVALGYKKVYYDPVR
                     KQFVSQAVIDKIKEIQNRWKDKYPNMDFKTQNLRFDTLINFNLTFTNEMEFLNMEPK"
BASE COUNT          496 a          466 c          399 g          672 t
ORIGIN      
        1 tgtttcagtt tctgcagggc ctgttctttg gtgaggtgtt ttttatacat gaattgtgat
       61 cagtgtaata ctggtatgca atggggttat tcttccagcc tggccgaaca accgaactca
      121 tgtttcaaat aatcatcata gtggtctgcc ttcttaaata agatccggaa ggcttctgct
      181 ccgttctctt tcgtcatgtc aaggtcgtag ataattcggc tgagtacaat attttgtttt
      241 acacaactta atactaatcc atgtgtgcgg taattttcct gcagcaggta ttgatacagc
      301 ggtttgatct ttgaaacatc agccggcatc tggcagagat aagcatcccc cgctatgaaa
      361 aaattttctg tattgtaggt gatctttatt ttggccgatc cttcttttac actccatgta
      421 ttaacccctt ccctggccaa ccgcacatct ttgcccagtt ctttcaatat ctcttcaatt
      481 gttttggcag cgccaaccgg ttccgcttct ttttcgggta tctccggcaa ctttacttct
      541 gtgccgcagc tcggacaata ttttcctgaa tcaatattgg cagcagtaac taaataacca
      601 caactgaaac aacgggcgga agcctgtccc ttattgggta aatacatttg cagggcttcc
      661 cgcaggtaaa gcaccggcaa tgcaaatcca agattatcac caccacggat gatgaatgaa
      721 ttgacaccta ttatttctcc gttcatattc accagcggac caccactgtt ccccggattg
      781 atagccgcat ctatttgtat aaactttaac ccgtctctta ttctatccac tttggagatt
      841 actccctgcg tggcggtata atttaaccca tatggatggc caatggccac tacggcatcg
      901 ccgtctttca tctgatcgta ctggcccagc cgaacttcgg gcagttcaat gtttttgggt
      961 gcttctaaaa atgccaggtc atgtttccgg tcggtatacc acactcttga cagtgcttta
     1021 tcaaatgttt ttccggcaat ggttacttcg gcgttttctg ccaccacatg atcattggta
     1081 actataaggt caaattcctt tatataaaaa ccagtccctg ttcccgtagc agttgctatt
     1141 tgaatgatgg cgttctggta tttttctatg atcagttgcg ggtccattac cattgattat
     1201 tttgattact tgggctccat gtttaaaaac tccatttcat tcgtaaacgt aagattgaaa
     1261 ttgataagcg tatcaaaccg gaggttctgt gttttaaaat ccatgttggg atatttatcc
     1321 ttccagcggt tttgtatctc ctttatttta tcaatgacag cctgcgaaac aaattgtttg
     1381 cgtacaggat cataatacac ttttttatat ccaagggcca caaagtattc cgggtagttc
     1441 accatcatat acaaatgaaa cagctcggta acagctttgg gcaggctgta agagtactgg
     1501 cagtaagaaa taccgtattc ggttatctgg ctggcaataa acggatagtt attgtcccgg
     1561 taccaaacca tgttctggtt agccgcatga atggcctcag ctaccgcctt gtatggctgt
     1621 ggcgccacga tggcaaacgt atatttggaa cgaaacaggt ttttaaaaac ttcttctttg
     1681 ggtaatgcct gcatattttt aaacgcttca gccatgtccc tgtttttttc tgttaccggt
     1741 ctttcgtcct ttttaaaata gataccggtt attttttcaa gctcattcag caattttcct
     1801 tccggtgtga tcatgtaatc tatccggtgt atcagcacca gcagtaaata cgctatcgct
     1861 cctgaaaatt tatctgcatc cagtggtgca atggtgtcca gtgtttcacc aatccatttt
     1921 tgcaggtaca tatacttcac ctctttctcc tgttccggaa cgaccgagat atgttcgtta
     1981 tctgattttt caagggcagt aaaatcctgt accagcaggt catcctgttt gtc
//
LOCUS       DAORTI010000289         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_88622_length_2024_cov_9.037583, whole genome shotgun sequence.
ACCESSION   DAORTI010000289 DAORTI010000000
VERSION     DAORTI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88622_length_2024_cov_9.037583"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1142
                     /locus_tag="PLO70_17385"
     CDS             69..1142
                     /locus_tag="PLO70_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76295.1"
                     /translation="MVAIAFSVITTSKTNAQGCVAIRSNGNTCSMSNPATAKGWQLNF
                     NNRYFKSYKHFVGTIEQKERAENHTEVINHSYSLDITATRYFNSRWSLAMTVPVISNT
                     RSSLYEHGGNSNGATARHSTQTFGIGDLRISAYRWLFDPAKHHKGNIQAGFGIKLPTG
                     DYRYQDYFYKSDGTTVMGPVDQSIQLGDGGTGFTFELNSYYNISHKVGIYGNFYYLVN
                     PREQNGVSTARGGTPNTIALQYFSSTMSVPDQYMARTGASFMTGKFNFTGGVRIEAIP
                     SSDLIGGDKGFRRPGMIIAAEPVVSYQAKKMNFYLSVPVALQRNRTQSYSDKLRTAAS
                     GTKIQGDAAFADYSINGGVSFRF"
     gene            complement(1234..1956)
                     /locus_tag="PLO70_17390"
     CDS             complement(1234..1956)
                     /locus_tag="PLO70_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQZ76296.1"
                     /translation="MTDKTLQQLKTAEIEKISHLDYFNIGITVDCVILGYDDKELKVL
                     LIKSDMQEFSGLYSLLGDFIKPDEDLETASYRILKERTGLDDVYLEQVHTFGGLNRHP
                     SGRVITIAYYSLIDIKNHKFQLSHNELHWHPVNEIRKLAFDHKLILDTCLNRLRQQVM
                     EHPVIFNLLPEKFSLRQLQELYEAILGVELDRRNFRKKIAIKDWLLDIDEMENDVPHR
                     PGKLYALKKQYKPKGAKALALY"
BASE COUNT          625 a          400 c          391 g          608 t
ORIGIN      
        1 cagcgtctcc aaatgaaaaa ataattgata acaaaaacca acgattaaaa atgaactatt
       61 tatttataat ggtagctatt gctttttcag taataactac ttcaaaaact aatgcacagg
      121 gatgtgtggc tatccgcagt aatggcaaca cttgtagtat gagtaatcct gcaacggcca
      181 aagggtggca gttaaatttc aataaccgtt atttcaaatc atataaacat tttgtaggaa
      241 cgattgaaca aaaagaaaga gcagaaaacc atactgaagt aatcaatcat agttattcac
      301 tagatattac cgctaccagg tattttaact cccggtggtc gttggctatg actgttcctg
      361 ttatttctaa cacccgttcg tctttatatg aacatggcgg caatagcaat ggtgcaacag
      421 caagacacag tacacagact ttcggtattg gggatctccg catcagtgcc taccgctggt
      481 tatttgaccc agccaaacat cacaaaggaa atatacaggc tgggtttggc atcaaactac
      541 caacaggaga ttaccgatac caggattatt tctataaatc tgatggaact actgtaatgg
      601 gccctgttga tcaatccatt caattaggag acggaggcac tggttttact tttgaactaa
      661 acagttacta taatatctcc cacaaagtag ggatttatgg aaatttttat tacctggtaa
      721 acccaagaga gcagaatggc gtatcaactg ccagaggcgg caccccgaat acgatagccc
      781 tccaatattt ttcaagtacc atgagtgtac ctgatcagta tatggcaaga accggcgcaa
      841 gttttatgac aggtaaattc aattttacgg gtggtgtacg cattgaagca atacccagca
      901 gcgacctgat tggtggcgat aagggtttta gaagacccgg aatgataata gctgcggaac
      961 ccgttgtttc ataccaggcc aaaaaaatga atttttactt atctgtacca gtagccttgc
     1021 aacgaaacag gacacagagt tattcagata aattgagaac ggctgcttcc ggaaccaaaa
     1081 ttcaaggtga tgcagctttt gctgattatt caataaatgg aggggtgtcg ttccgctttt
     1141 aaaactggca gaataagaca attataaaat ggcgttgtaa agagttgcaa cgccatttta
     1201 tttgctttcg atattgtaat caaaataaaa aaatcagtaa agtgccagtg cttttgctcc
     1261 ttttggctta tactgtttct ttaatgcata taatttaccc ggccggtgag gcacatcatt
     1321 ttccatttca tcaatatcca acaaccaatc tttgatagct attttttttc tgaaatttct
     1381 acggtccagt tcaacaccca atatggcttc atacaattcc tgcaattgac gcaacgaaaa
     1441 tttttccggc agtaaattga aaattaccgg gtgttccatt acctgttgcc ggagcctgtt
     1501 caaacaggta tcaagaataa gtttatgatc gaaagccagt tttcttattt cattcaccgg
     1561 gtgccagtga agttcattgt gacttaattg aaacttgtga tttttaatat caatcagtga
     1621 ataataagca attgtaatta cacggccgga agggtgccgg ttaagacctc cgaatgtatg
     1681 tacctgctcc aggtatacat cgtcaagtcc tgttcgttct ttcagtattc tgtaagaggc
     1741 tgtttccagg tcttcatcag gttttataaa atcgccaagc agagaatata gcccggaaaa
     1801 ttcctgcata tcagacttga tcagcaaaac tttcagctct ttatcatcat atcccaatat
     1861 cacacagtca acagtgatac caatattaaa ataatcaagg tggctaatct tctctatttc
     1921 ggccgtcttc agttgttgta aggttttgtc cgtcattgca atcgaatttg aaattattga
     1981 atggccagtt tgacctgcgt ttttttatta gctgcttgta tgct
//
LOCUS       DAORTI010000290         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_89143_length_2017_cov_7.639144, whole genome shotgun sequence.
ACCESSION   DAORTI010000290 DAORTI010000000
VERSION     DAORTI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89143_length_2017_cov_7.639144"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="PLO70_17395"
     CDS             complement(<1..>2017)
                     /locus_tag="PLO70_17395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQZ76297.1"
                     /translation="YDALNRVSSENFGTRAVHYTYNISGRTQTTIYPDSTVVTKSYDT
                     RNRLSSIAKNNTVLVSYQYNNANQVIAKTMANGVTTSYQYDFANRLSSISTAGGSIQN
                     NYFTYNNEQQKTSINRLNDPAKSEQFTFDNGRRLTNYKRGVIGGSPVIENIYNYDALG
                     NRTAANQNGINTTYTGNNLNQLTNSNNGTQNINFTYDNNGNLTYDGQFFKFYDAEKRL
                     LKDSSSPVNIITYQYDALNRRTRKNINGTTRNYTFSGITPIEERDNAGNTILNKTIYA
                     GWLRPVVNEKDNNQYFYHQNELNSIEAITNQQGRLIEKYDYDVYGKMNIYDSLNNPLP
                     GSIAGNRFGFTGQEYDSATGMYKFFFREYNPETGLFSQRDLIGYADGMGMYQYVHNNP
                     ANGVDVLGLEDCPENKEQNDFNNSVKSVEFVAGWENFLFSAYEVLHNEINQYNQNKAL
                     EELNQLQKEWAWYKAKSMMKGGKEGAKNLNKANKILEQMKNVKVPDGSKIAKVANGLD
                     KVGKGLNVLDLVIKGHSFGEAINNYNNGTIDGAELSLSGGNLAQSALNFTGIGSAYSF
                     ADFLQQQATGKSMNEWSAEYGQTFGENDVDQNMDEELMEFHRKNGTLKKFLKFYHKTN
                     SKEIRKRRADCPQNNNGGTQKPAGPSYDPNSTTEAIASWDPNEIIGPD"
BASE COUNT          495 a          421 c          381 g          720 t
ORIGIN      
        1 catcgggccc gatgatctcg ttagggtccc atgatgcgat ggcttcggta gtggaatttg
       61 gatcatatga gggtccggca ggtttttggg taccgccgtt attattctga gggcaatcgg
      121 cccgcctttt cctgatttct ttactatttg ttttatgata gaacttcagg aattttttaa
      181 gtgttccgtt tttgcggtga aactccataa gctcttcatc catattttga tctacatcat
      241 tttcgccaaa agtttgtcca tattcggcag accattcatt catactttta cctgtagcct
      301 gttgctgtaa aaaatcagcg aaactatatg ccgacccaat cccggtaaaa ttaagggcac
      361 tttgtgctaa attaccaccg gaaagactta attccgctcc atcaattgta ccattattat
      421 aattattgat agcctctcca aaactgtgac cttttatcac gagatcaaga acatttaatc
      481 ccttaccgac cttatccagc ccattcgcta cttttgcaat tttggaaccg tctggcactt
      541 ttacgttttt catttgttct agaattttat ttgctttatt taaattcttt gcaccttctt
      601 tccctccttt catcatgctt tttgctttat accatgccca ttctttctgt aactgattta
      661 gctcctccaa ggctttgttt tgattgtatt ggttgatttc attgtgtaat acttcataag
      721 cactaaaaag gaagttttcc caacctgcaa cgaattctac agattttaca ctgttgttaa
      781 aatcgttttg ctccttattt tccggacaat cctcaagtcc cagcacatca actccattcg
      841 ccgggttatt atgtacatac tggtacatgc ccattccatc agcatagcct atcaaatccc
      901 tttggctgaa taagccagtt tcaggattgt attcacgaaa gaaaaacttg tacatgcctg
      961 ttgcgctgtc atattcctgg ccggtaaagc caaatcggtt acctgcaatt gaacccggca
     1021 aaggattatt cagcgaatca taaatgttca ttttgccata cacatcgtaa tcatattttt
     1081 caataagtct tccctgctgg ttggtaatgg cttctattga gttcagttcg ttctgatgat
     1141 agaaatactg gttgttgtcc ttttcattca ccaccggtct cagccaacct gcataaattg
     1201 ttttattcag tatagtgtta ccggcattat ctctttcttc gatgggagtg atgcctgaaa
     1261 aagtatagtt gcgggtagtg ccatttatat ttttacgggt tctcctgttc aatgcatcat
     1321 actggtaagt gataatattg acaggcgagg aagaatcttt taacaatctc ttttcagcat
     1381 catagaattt aaaaaactgc ccatcatagg tgagattgcc attgttgtca taagtaaaat
     1441 tgatattttg agtaccatta ttactgttgg ttagctggtt caggttattg ccggtgtacg
     1501 ttgtatttat accattctga ttggcagcag ttctatttcc gagcgcatcg taattgtata
     1561 tattctcgat tacagggctt cctccgataa cccctctttt ataattggtt agcctgcggc
     1621 cgttatcgaa agtaaattgt tccgatttgg ccggatcgtt cagccggtta attgaagttt
     1681 tctgttgttc gttattatag gtaaagtagt tgttttgtat ggaaccgcct gcagtagaga
     1741 tgctgctcag ccggttagca aaatcatatt gataggaagt ggttactccg ttagccattg
     1801 tttttgcgat gacctggttc gcattattat actggtaact aaccaatact gtattgttct
     1861 ttgcaatact gctgagccgg ttacgggtat cataactttt tgtgactacg gtactatccg
     1921 ggtagatggt tgtttgagtt cttccggaaa tattatacgt atagtgtaca gctctggttc
     1981 caaagttctc agaactgacc ctgttcaatg catcgta
//
LOCUS       DAORTI010000291         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_89146_length_2017_cov_6.636086, whole genome shotgun sequence.
ACCESSION   DAORTI010000291 DAORTI010000000
VERSION     DAORTI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89146_length_2017_cov_6.636086"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PLO70_17400"
     CDS             complement(<1..1453)
                     /locus_tag="PLO70_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQZ76298.1"
                     /translation="MVFDLDLIKKVYAEMPAKIDAARKLTGRALTMTEKILYSHLYGA
                     LPSVEHGRGSDYVDFAPDRVAMQDATAQMALLQFSTAGRSKVAVPSTVHCDHLIQAKV
                     GAKKDLELAIETNREVYDFLSSISDKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTPNAGGLGMVAIGVGGADAVDVMAGLPWELKMPKVIGVKLTGKLSGWTSCKDVILW
                     VAGQLTVKGGTGAIVEYFGEGADSMSATGKGTVCNMGAEIGATCSLFAYDEKMSSYLK
                     ATSRKEVAALADGIKEHLRPDAEVYANPAEYYDQLLELDLSTLEPYVNGPFTPDLATP
                     ISKMAEAVEKNGWPAKLEVALIGSCTNSSYEDISRSASIVKDAVSQGIKTKSEFTITP
                     GSELVRYTIERDGFIKEFEDAGGVVLANACGPCIGQWARHIDDPNRKNTIITSFNRNF
                     AKRNDGNASTHAFVASPEIVTAMAMAGTISFNPL"
     gene            1559..1780
                     /locus_tag="PLO70_17405"
     CDS             1559..1780
                     /locus_tag="PLO70_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HQZ76299.1"
                     /translation="MNKLKSFVEWNAFGVCSAIGNRMGIATSSIRRYFMYASILTVGS
                     PILIYMVLAFWRNMRKYIWAAKRNPWYYL"
BASE COUNT          562 a          451 c          343 g          661 t
ORIGIN      
        1 ttaatggatt aaaagagatt gttccggcca ttgccattgc tgttacaatt tcaggacttg
       61 caacaaatgc atgggtactt gcattaccat cattacgctt cgcaaagttt ctgttgaatg
      121 aagtgatgat agtattcttc cggtttggat catcaatatg tcttgcccac tgaccaatac
      181 aaggtccgca agcatttgct aatacaactc caccagcatc ttcaaattct ttaatgaaac
      241 catctctttc aatcgtatat cttaccaatt cacttccggg agttatggtg aattcagatt
      301 tggtttttat tccctggctc actgcatctt ttacaataga agcactacgg cttatatctt
      361 catatgatga attggtacaa gaaccgatca atgctacttc cagttttgca ggccagccat
      421 ttttttcaac agcttctgcc attttagaaa ttggtgttgc aagatccgga gtaaacggtc
      481 catttacgta tggttccaat gtgcttaaat ccaattccaa taattgatca taatactcag
      541 caggattcgc atacacttca gcatccggac gaagatgctc ttttattcca tctgctaaag
      601 cagctacttc tttacggctc gttgctttca aatagctact cattttttca tcgtaagcaa
      661 acaacgaaca ggtagcacct atttcagcac ccatattaca aactgttcct tttccggttg
      721 cactcatgct gtctgcacct tcgccaaagt attctacaat ggcaccagtt ccacctttta
      781 cagtcaactg accagccacc caaagaatca catctttaca agatgtccag ccactcaact
      841 ttccagtcaa cttcacacca atcacttttg gcatttttaa ttcccatggc aaaccagcca
      901 ttacatccac tgcatcagca ccaccaactc ctattgccac cattcctaaa ccacctgcat
      961 ttggtgtatg cgaatcggta ccaatcatca ttccaccggg aaatgcataa ttttctagaa
     1021 caacctggtg aataattcca gctcccggtt tccagaagcc gattccgtat ttatcagata
     1081 tagaagaaag aaaatcatat acttctctgt ttgtttcaat cgccaattct aaatccttct
     1141 tggctccaac ctttgcctgt ataaggtgat cgcaatgcac tgttgatgga acagccactt
     1201 ttgaacggcc agccgtactg aattgcaaca aagccatttg agcagttgca tcctgcatag
     1261 ctactctatc tggcgcaaaa tccacataat cacttcctct gccatgttct accgaaggca
     1321 atgcgccgta gagatggcta tataatattt tttctgtcat tgttaatgcc cttccagtca
     1381 attttctggc tgcatcaatt tttgcaggca tttcagcgta tactttctta atgaggtcta
     1441 aatcgaatac catgtggaga gtcttttgaa gtgagtaaat gtgcggcgaa tttacgaata
     1501 aacagggtgt taataaagct ttattgcttg ttttcgggca attttctact ttagtggtat
     1561 gaacaaatta aaaagctttg tagaatggaa tgcctttggg gtttgttcgg ccattggcaa
     1621 taggatggga attgccacca gcagcatccg caggtacttt atgtatgcct ctatccttac
     1681 agtaggttcg ccaattctta tttacatggt actggctttc tggcggaata tgagaaaata
     1741 tatttgggca gcgaaaagga atccttggta ttatctgtaa gagaattaaa cttattggcg
     1801 ataataaaaa aggactgaat taatttccag tcctatcgta ttcataaaag tttttttctt
     1861 ttagttcttt tttgatgtaa ttataattac accatctttc cctttctcag catataagtc
     1921 tgtagcgttt tttcctttca atacattaat tgattcaatt gaatgagggc ttaaattatc
     1981 catttcttgt tgtgaaataa ttttgccatc caataca
//
LOCUS       DAORTI010000292         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_92207_length_1977_cov_6.229969, whole genome shotgun sequence.
ACCESSION   DAORTI010000292 DAORTI010000000
VERSION     DAORTI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92207_length_1977_cov_6.229969"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..>1977)
                     /locus_tag="PLO70_17410"
     CDS             complement(211..>1977)
                     /locus_tag="PLO70_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQZ76300.1"
                     /translation="WISEYVLAGYGTGAIMAVPCGDERDHKFARHFNIPITNIIGSFY
                     DGEEANPTKEAVLENSGFLNGLVMKDAMEVAIKAIEEKGIGTRKVNFKMRDAAFSRQR
                     YWGEPFPIVWKNGIAYPLDEKELPLELPHVDSYKPGPDGEGPLANIPEWVERELETNT
                     MPGYAGSSWYFLRYMDPHNDKAFCDRKASDYWNQVDLYIGGTEHAVGHLLYSRMWTKV
                     LYDLGHIGFDEPYKKLVNQGMIQGSSRFVYRVAGYQISGSEAEVNLGSKKPVYISYNI
                     YNEFRGLDPKSDRAVEISSLILKHIYGKEVNERDVIFKIIQHHVDVYLVDGFVLDKVA
                     FKKWREEYAEADFILDGDDYICGAEVEKMSKSKYNTVNPDDLVMKYGADTFRMYEMFL
                     GPVEMSKPWDTKGIEGVHRFLKKFWRLFAEETKGLIVTNDEPTAEEMKVLHKTIKKIE
                     EDTERFSFNTAVSGFMIATNELTDLKCHKRSVLEPILILLTPYAPHIAAELWNLLGHS
                     DSILDAAYPKFEAKYVAETSKEYPISINGKLRTTMNIALDAAQPEVEKMVLENEVVQK
                     WLEGRPPKKIIFVKGKMVNVVV"
BASE COUNT          558 a          480 c          324 g          615 t
ORIGIN      
        1 catagacaat ttgttaggta taaaacgggt atgaagatgt aattgtttga taaaataaaa
       61 aaaagaggct gtctcaaaag ccccaatcag tgtcatattg agcatagccg aaatatgaca
      121 ctgattggta aacaaatttg tacgcatttc gataaactca atgtgacaaa tttgtttgca
      181 tttgagacag cctcatttca ctttgttgaa ttataccacc acattcacca tcttgccctt
      241 cacaaaaatg atcttcttcg gtggtctgcc ttccagccat ttttgcacca cttcattttc
      301 caataccatt ttctctactt caggctgtgc tgcatccaat gcaatattca tcgtggtacg
      361 caacttacca tttattgaaa tggggtactc cttacttgtt tctgctacat atttagcttc
      421 gaatttggga taagctgcat ccagtatact gtcgctatgc cccagcaaat tccacaattc
      481 agctgctata tgcggagcat agggagtaag caggatcaat atcggttcga gcacagaacg
      541 tttatgacat ttcagatcgg tgagttcatt ggtggcgatc ataaaaccgc ttactgcagt
      601 attaaaactg aaccgttcgg tatcttcttc tatcttctta atggttttgt gcaatacttt
      661 catttcttct gcagtaggtt catcgttggt aacgatcaat ccctttgtct cctctgcaaa
      721 taagcgccag aattttttca gaaagcggtg cactccttca ataccttttg tatcccatgg
      781 cttgctcatc tcaaccggac ccaggaacat ttcatacata cggaaagtat cggcgccgta
      841 tttcattacc aaatcatctg ggttaacggt attgtatttt gatttggaca ttttctctac
      901 ttcggcaccg cagatataat catctccatc taaaataaaa tctgcttcag catattcttc
      961 tctccatttt ttaaaggcta ctttatctag tacaaatcca tccaccaaat agacatcaac
     1021 atgatgctga attatcttga aaataacatc tctctcatta acttctttac cataaatatg
     1081 ttttaaaatt aaacttgaaa tctcaactgc tctatcactt ttaggatcta atcctctaaa
     1141 ctcattataa atattataag atatatagac aggtttttta cttcccaaat taacttcagc
     1201 ttcacttcca cttatttgat aaccagcaac cctataaaca aacctcgaac ttccctgtat
     1261 catcccctgg ttcaccaatt tcttatacgg ctcatcaaaa ccaatatggc cgagatcgta
     1321 caatacttta gtccacatgc ggctgtataa taaatgtcca acagcatgtt cggtaccgcc
     1381 gatataaaga tccacctggt tccagtaatc agaagctttt ctgtcgcaaa atgctttatc
     1441 attatgcgga tccatatagc ggagaaaata ccaggatgaa ccggcataac cgggcatagt
     1501 gttcgtctcc agttcccttt ctacccattc cggaatatta gccagtggtc cttcaccatc
     1561 cggaccaggt ttatagctgt ccacatgcgg caactctaat ggcagttcct tttcatccag
     1621 tggataagca ataccatttt tccaaactat cggaaaaggc tcaccccaat aacgctgccg
     1681 gctgaaagcc gcatcccgca ttttgaaatt cacctttctc gtacctattc ctttttcctc
     1741 gatggctttg atcgccactt ccatagcatc cttcatcacc agcccgttca ggaaaccgga
     1801 gttctccaaa actgcttcct tggtaggatt ggcttcttcg ccgtcgtaaa atgaaccgat
     1861 aatattagtg atgggaatat tgaaatgccg ggcaaacttg tgatcccgtt catccccgca
     1921 gggaacggcc atgattgcac cggtaccata accagccagt acatattcag atatcca
//
LOCUS       DAORTI010000293         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_95463_length_1937_cov_6.120616, whole genome shotgun sequence.
ACCESSION   DAORTI010000293 DAORTI010000000
VERSION     DAORTI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95463_length_1937_cov_6.120616"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLO70_17415"
     CDS             <1..558
                     /locus_tag="PLO70_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76301.1"
                     /translation="IRYSAFRGGDGSGENGIKPPALSVSVHSEAFNLSLEKVLNAYYE
                     MNTGFAENNIASINEQGTGLKISLDSLKLEDLEKDSLIYQTALQPYENAKAEINSIIE
                     DPSIEEKRGSLNILSNELFAFLTTVRYDQAVLYWLECDQAFGDGKPGNWISKQEKAQN
                     PYGQEDCRDIKTKINFIKTDSTKVK"
     gene            566..>1937
                     /locus_tag="PLO70_17420"
     CDS             566..>1937
                     /locus_tag="PLO70_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQZ76302.1"
                     /translation="MAIMPKFISAVILLLFFSTVFSQNRFTINGYVKDSLSGESIIGA
                     TIAVNGQSRGVASNQYGFYSITLEEGNYTLNVSHVSYLGKSIEINLRNNQSFNFEILS
                     RSAAIEEVVVFSKRRDGNVKNAQMGKIDLSMNQVRNIPAFLGEVDILKAIQLLPGVQS
                     AGEGNAGFYVRGGGPDQNLIMLDDAVVYNTGHLFGFFSIFNGDAIKNVSLIKGGMPAQ
                     YGGRLSSVLDVSMKDGNINKFQTEGGIGLIASRFSIQGPLKKEKASFILSARRTYVDA
                     LAKPFIKKSSSFYGSGYYFYDVNAKVNYRFSEKDRLYLSGYFGRDVFDFNNSKRSFKT
                     NIPWGNSTATLRWNHVFNRRLFSNTTVVYNDYNFKFSAQQENFNIGLSSGIRDYTLKS
                     DFDYYPLPDHKLKFGGLVTYHKFIPNVVTGRQDSIEFRPNNEGAKYAAETALYLQDDW
                     ELGEKWK"
BASE COUNT          613 a          349 c          413 g          562 t
ORIGIN      
        1 atccgttatt cagcatttag gggaggagat ggctccggag aaaatggtat taaaccacct
       61 gccctgtctg tcagcgttca tagtgaagct tttaatctgt cactggaaaa ggtactgaac
      121 gcctactatg aaatgaatac cggttttgcc gaaaataata tagcgtccat aaatgaacag
      181 ggaactggtt tgaaaatctc cctggatagc ctgaaactgg aagatctgga aaaggatagt
      241 cttatttacc agactgcgct gcaaccctat gaaaatgcaa aagctgagat caattcaatt
      301 atcgaggatc cgtcaattga agaaaaaaga gggtcattga atattctttc caatgaacta
      361 tttgcgtttt taacaactgt ccggtatgat caggccgtat tatactggct cgaatgcgat
      421 caggcatttg gtgatggaaa gcccggaaac tggataagca aacaggaaaa agcacagaat
      481 ccatatggcc aggaagactg cagggatata aaaacgaaga tcaattttat caagacagat
      541 tcaactaaag taaagtaaga ttgttatggc tataatgcca aaattcatca gcgcagttat
      601 acttcttctt tttttcagta ctgtattctc acaaaatcgc tttacaataa acggatatgt
      661 gaaagacagt ctttcaggtg agtcaatcat cggagctact attgctgtaa acgggcaatc
      721 caggggagta gccagcaatc aatatggttt ttattcaatt accctggaag aagggaatta
      781 tacactcaat gtatcacatg tttcctacct cggtaagtca atagaaatta atctccgaaa
      841 caaccaatct tttaattttg agatcctttc caggtctgct gcaattgaag aagtggtagt
      901 ttttagtaaa cgcagggatg gtaatgtgaa aaatgcacaa atgggaaaga ttgatctttc
      961 catgaaccag gtgagaaata taccagcctt tttgggtgaa gtggatattt taaaagctat
     1021 tcaattgtta cccggtgtgc aaagtgccgg agaaggtaat gcaggctttt atgtacgtgg
     1081 gggcggtccc gatcaaaatc tgatcatgct ggacgatgct gttgtttaca atactggtca
     1141 tctctttggt ttcttttcca tttttaatgg agacgccata aaaaatgtat cccttataaa
     1201 gggagggatg cctgcccaat acggaggacg tctctcttcc gtgcttgatg tttccatgaa
     1261 agatggaaat atcaataaat tccaaacaga aggaggaatt ggtttaattg cttcccgttt
     1321 ctccatacaa gggccactaa aaaaagagaa agcctccttt atcttgtcag caaggagaac
     1381 ctatgttgat gccctggcaa aacctttcat aaaaaaaagc agcagcttct atggttccgg
     1441 ttattatttc tatgatgtga atgctaaggt caattatcgg ttttcagaaa aagatcgttt
     1501 gtacctgagt gggtattttg ggagggatgt gtttgatttc aataattcaa aacgttcttt
     1561 taaaacaaat ataccctggg gaaattcaac tgcaactctt agatggaacc atgtatttaa
     1621 ccggcggctt ttttcgaaca caacagtagt atacaatgat tataatttta aattttctgc
     1681 ccaacaggag aattttaaca tcgggctttc ctctggtatc agggattata ctctaaaatc
     1741 tgattttgac tattatccat tacctgatca caaactcaag tttggcggcc ttgttactta
     1801 tcataagttt attccaaatg tagttaccgg caggcaggat tcgattgaat tcagacccaa
     1861 taacgaaggg gctaaatatg cagctgaaac ggcactttat ctgcaggatg actgggaact
     1921 gggagaaaaa tggaaga
//
LOCUS       DAORTI010000294         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_96143_length_1929_cov_5.728922, whole genome shotgun sequence.
ACCESSION   DAORTI010000294 DAORTI010000000
VERSION     DAORTI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96143_length_1929_cov_5.728922"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLO70_17425"
     CDS             <1..795
                     /locus_tag="PLO70_17425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQZ76303.1"
                     /translation="QLLHDTPDGSWGAMGSMITSIDEFAKYVAFHLSAWPPSNAAEKG
                     PVKRSAVREMHHPWRWNGFNPNFKYPDGRICAVTSAYCYGLGWTKDCDGKTYISHSGG
                     LPGFGSQWRIMPEYGIGVVAFANRTYSPFGTVNLRVLDTLIKLAGLKPSQLPPSAILE
                     KRKNELVKLLPDWNNAEQTGIFAENFFPDYPIDTLKKQARDLYTKAGKIIAIKEMKPE
                     NQLRGSFILQGEKADIQIYFTLSPENPPLIQEYHIRELPKEKILSP"
     gene            806..1102
                     /locus_tag="PLO70_17430"
     CDS             806..1102
                     /locus_tag="PLO70_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76304.1"
                     /translation="MYIVREIFHLQFGRYREAKTLIDEGMQKHLLLQPSGSRILTDFT
                     GEGYRLIIELPYATLADYETDLKKELGGTGWKDWYEKFKALIRYSEREIMKQVM"
     gene            complement(1103..>1929)
                     /locus_tag="PLO70_17435"
     CDS             complement(1103..>1929)
                     /locus_tag="PLO70_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQZ76305.1"
                     /translation="EPEYRPEEPIDVGEIEHLSFNNVTFHHRNATTNALENISFEAGI
                     GDTIAFVGPSGSGKSTLVKLLVGLYTPVTGDILYDNISSRDIRYNRMRRQLGFVTQDT
                     QLFSGTIRDNMRFVKPDATDEEINAALKQASASNIVYSNGRGLDTVLGEGGRKVSGGE
                     KQRLSIARALLRKPRLLIFDEATSALDSLTEEQITATVKEISALKQQITILIAHRLST
                     IMHADIIYVLEKGKIVEQGTHDELVDKKGLYYAMWRQQIGERKSFIASKTTDQTSE"
BASE COUNT          566 a          388 c          422 g          553 t
ORIGIN      
        1 caattattac atgatacacc ggatggcagt tggggagcga tgggcagtat gatcacttcc
       61 attgatgaat ttgcaaaata tgtggctttt catttatcag cttggcctcc ttccaatgct
      121 gcagaaaaag gcccggtaaa aagaagcgct gtcagggaaa tgcatcaccc gtggaggtgg
      181 aatgggttta atcccaattt caaatatcct gatggaagaa tttgtgctgt tacatccgct
      241 tattgttatg ggttgggatg gacgaaagat tgtgatggca aaacatatat ttctcacagt
      301 ggtggcttgc cgggttttgg cagtcagtgg cgcatcatgc ctgaatatgg aatcggggtg
      361 gtggcgtttg cgaaccgtac gtattcaccg tttggtactg tcaatttacg ggtacttgac
      421 acactgatca aattggcagg cttgaaaccc agccagcttc cgccatcggc tatactggag
      481 aaaagaaaaa atgagttggt aaaattatta cccgactgga acaatgcaga acaaactggg
      541 atctttgcag agaatttctt tcctgattac ccgattgaca cgttgaagaa gcaggcaaga
      601 gacctgtaca cgaaagccgg aaagatcatt gcgataaaag aaatgaaacc ggagaaccag
      661 ttacggggct catttatttt gcagggagag aaagcagaca ttcaaatata tttcacttta
      721 tcacccgaaa atccgccact gatacaagag taccatatca gggaattgcc gaaagaaaag
      781 attctatcac cttaaagcaa ctgttatgta cattgtccgt gaaattttcc acttgcagtt
      841 tggccgctac cgcgaagcca aaacactgat tgatgaaggc atgcaaaaac atttactatt
      901 gcaaccatcg ggaagccgca tccttaccga ttttaccggg gaaggttatc gtctcatcat
      961 tgaattaccg tatgccactt tagccgatta tgaaaccgat ctgaaaaaag aacttggtgg
     1021 caccggctgg aaagactggt atgaaaaatt caaagccctg atacgttatt cggaaagaga
     1081 aataatgaag caggtaatgt aattattcac tggtttggtc ggttgtctta ctggcgataa
     1141 aagattttct ttcaccaatt tgctgccgcc acatggcgta gtacaatcct tttttatcca
     1201 ccagttcatc atgggtgccc tgttcaacaa tttttccttt ttccaataca taaataatgt
     1261 cggcatgcat gatagtagaa aggcggtgtg caatcagaat agtgatttgt tgttttaatg
     1321 cagaaatttc tttgacggtt gctgtaattt gttcttctgt taaagaatct aatgcagaag
     1381 tagcttcatc aaaaatcaac agccgtggtt ttcgaagcaa tgctcttgcg atagagagac
     1441 gttgtttttc accgccggaa acttttctgc ccccttcacc caacactgta tctaatcccc
     1501 tgccattact gtacacaata ttggaagcag aagcctgttt taatgctgca tttatttctt
     1561 catctgtagc atctggtttt acaaacctca tattatcccg tatagtacca gaaaataatt
     1621 gggtgtcttg tgttacaaaa cctaattgcc ttctcatccg gttgtaacgg atgtcacggg
     1681 aggaaatatt atcgtataaa atatcaccgg taacgggagt gtaaagacca accaataatt
     1741 ttaccaacgt tgatttgccg gaaccggaag ggcctacaaa agcgatggtg tcaccgatac
     1801 cggcttcaaa agaaatattt tcaagtgcat tcgtagtagc attacgatga tggaatgtga
     1861 cattattaaa gctaagatgt tcaatctctc ctacatctat tggttcttcg gggcgatact
     1921 ctggttctt
//
LOCUS       DAORTI010000295         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_104548_length_1838_cov_5.065059, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000295 DAORTI010000000
VERSION     DAORTI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104548_length_1838_cov_5.065059"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLO70_17440"
     CDS             complement(<1..375)
                     /locus_tag="PLO70_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76306.1"
                     /translation="MKILLSFLFYSLLFIPFTVSAQNEPSPEAEAFYKKAMAEINAKH
                     VRWIKTTAAEVNEKKLSETDLMNRSKSYGTSGNLNGQDIEAIAFLVLMQASKSAQEDL
                     KAIMAQVKALNEQKAKQRELLSK"
     gene            527..>1838
                     /locus_tag="PLO70_17445"
     CDS             527..>1838
                     /locus_tag="PLO70_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76307.1"
                     /translation="MKKQSCSTPYHSPCFFYFLLSFLLVLSFGSYSQPGNIATIKALE
                     KEMITAYKNKEFESILRIGDSMLQIAPANKRVLGSCYDVAWKELGDTLKALDYLRMAV
                     AQHPFDPFFLTNYSWLLLVNGQAIKALPVCKKAYTFDRSSVAAIVNYAHCMYIMGIKS
                     IANEMYDEAMALTSSEEEFIQAQEADFNLLHSLYPDAGFDQLREAQRKKLSEALVYYQ
                     TANQLNREFTAINVKGKATVTEVLSKINEALGAELAREPYRLKRISKYLTTSGNTTYK
                     TGQVKTAIQQYQQAIPYNLQLNDNSELGKIYFNIGVIYKNIRTLDSAYYYFDGAAYYY
                     SLAGDKINEAGAYDAMARVYFINDETEKAFPIIKDKVIPLMEKYNAYEDIIDAYLFMS
                     DYYRDKKQDDSVQYYLEKSVETAGINSVQDDKLATLYNQLAVDAA"
BASE COUNT          565 a          373 c          370 g          530 t
ORIGIN      
        1 ttttgaaagt aattcccgct gctttgcttt ttgttcattc agtgctttta cctgggccat
       61 aattgctttc aggtcttcct gcgcactttt agaagcctgc atcagcacaa gaaaagcaat
      121 tgcttcaata tcctggccgt tcagattacc cgatgtgccg tacgattttg accggttcat
      181 taaatctgtc tctgacagtt ttttttcgtt cacttccgct gcagtagttt ttatccacct
      241 tacatgcttt gcatttatct ctgccatcgc ttttttatag aaagcctccg cttccggtga
      301 gggttcattt tgggctgata cagtaaaggg aataaataga agtgagtaaa acaggaagga
      361 taataatatt ttcataacct aaagtttaga tattgaagtt ccttttttcc gggggattta
      421 tgaaaacaag tttaaagaat cctgtatgac cataatctta catttgctga ataaaaagaa
      481 gccaacccca cagtgaggag atattcaatt ggttaaaccc agcgttatga aaaaacaatc
      541 ctgctcaacc ccctatcatt ctccatgctt cttttatttc cttttgtcct ttctcctggt
      601 tctttctttc ggcagctatt cccaaccggg aaatatcgcc acgatcaagg cattggaaaa
      661 agaaatgatt actgcctaca aaaacaaaga atttgaaagc attttaagga tcggggattc
      721 tatgctccag attgcaccgg ctaacaaaag ggttttaggc tcctgttatg atgtggcctg
      781 gaaagaactt ggagacactt taaaagcatt ggattacctg aggatggcag ttgcacagca
      841 tccatttgat ccgttctttt taaccaatta tagctggtta ttgctggtta acgggcaagc
      901 cataaaggct ttgcccgttt gtaaaaaagc atacacattc gacaggtcat ctgttgctgc
      961 catagtcaac tatgcccatt gtatgtatat aatgggaata aaaagcattg ccaatgaaat
     1021 gtatgatgaa gcaatggcac tgaccagcag cgaagaagaa ttcatacagg cccaggaggc
     1081 agatttcaat ttgttgcata gcctgtaccc ggatgcaggt tttgatcagc tcagggaagc
     1141 acaacgaaag aaattgtcag aggcgctagt ttattatcaa actgctaacc aattgaacag
     1201 ggaatttaca gccatcaatg tgaaaggcaa agcaacagtt actgaagttt tgtcaaaaat
     1261 aaatgaagct cttggtgctg aactggcccg ggaaccctat cggttaaaac ggatcagtaa
     1321 atacctgacc acctccggaa atacgactta taaaaccggt caggtaaaaa cagccattca
     1381 acaataccag caagccatcc cctacaacct tcagttaaat gataattcag aacttggaaa
     1441 gatttacttc aatatcgggg tgatatataa aaatatccgg acactggatt ccgcctatta
     1501 ttattttgat ggtgcagctt actattattc tttagccggt gataaaataa atgaggccgg
     1561 cgcctacgat gcaatggcaa gagtatactt tataaatgat gaaacagaaa aagcattccc
     1621 cataataaag gataaggtga tcccactcat ggaaaaatac aatgcgtatg aggacatcat
     1681 agatgcttat ttatttatga gcgactatta ccgggataaa aaacaggatg attctgttca
     1741 gtattatctt gaaaagagtg tggaaacggc tggcattaac tccgtacagg atgataagct
     1801 ggccaccctg tataaccagc ttgcagttga tgccgccg
//
LOCUS       DAORTI010000296         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_105159_length_1832_cov_5.909961, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000296 DAORTI010000000
VERSION     DAORTI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105159_length_1832_cov_5.909961"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLO70_17450"
     CDS             <1..420
                     /locus_tag="PLO70_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76308.1"
                     /translation="SSMVNSSQARVALELEKPEFQQAVQKLINELLLYAKDNKQEDFG
                     SNLVYNGNDKNRAWKSPVNMSEATEAKLAIEMMGNVNRAMADCSGFTFGKLQADKESE
                     GYWIVQPLQCGKKIIRFAFLKVKDKLLLGDIDLEQMN"
     gene            509..1303
                     /locus_tag="PLO70_17455"
     CDS             509..1303
                     /locus_tag="PLO70_17455"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQZ76309.1"
                     /translation="MQEYLNLLRHIIDTGTHKTDRTGTGTQSVFGYQMRFDLQKGFPL
                     VTTKKVHLKSIIYELLWFLKGETNIAYLKEHGVKIWDEWADEKGELGPVYGKQWRSWE
                     GKDGKIVDQISELITQIKKNPDSRRLIVSAWNVADLPEMALMPCHNMFQFYVANGKLS
                     CQLYQRSADVFLGVPFNIASYALLTMMIAQVCDLEPGEFVHTFGDVHIYSNHKEQVNL
                     QLSRNPFPLPVMKLNPDVKDIFSFQFEDFTLENYQSHPPIKAPVAV"
     gene            1345..>1832
                     /gene="folA"
                     /locus_tag="PLO70_17460"
     CDS             1345..>1832
                     /gene="folA"
                     /locus_tag="PLO70_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HQZ76310.1"
                     /translation="MTISLVVAAAANNAIGKDGIMPWHLPNDMKHFKNVTWGMPVVMG
                     RKTFDSLGKALPGRKNIVITRQSGWKAAATVVVKSIDDALFVAKEADANEVMIIGGGE
                     IYRALFDKAKRIYLTRVEAEPEADTFFPSLDQEHWHLVSQKKHEADGKNAYNYSFEIW
                     ER"
BASE COUNT          557 a          333 c          449 g          493 t
ORIGIN      
        1 agctcaatgg taaacagttc ccaggcaaga gtggcacttg aactggagaa gccggagttt
       61 caacaagctg ttcagaagtt aataaatgag ctcctgctct acgcaaaaga caacaagcag
      121 gaagattttg gttctaattt ggtttataac gggaatgata aaaaccgggc atggaaatcg
      181 ccggttaata tgagtgaagc aacggaggcc aaactggcga ttgaaatgat gggaaatgtt
      241 aaccgggcta tggcagattg cagcgggttt actttcggca agctacaggc tgataaagaa
      301 tcagaaggat attggattgt tcagccgtta caatgcggaa aaaagatcat tcgcttcgcc
      361 tttttgaaag taaaagataa attgttgctg ggtgatatag atttggaaca gatgaattaa
      421 aaaagcagaa gccggtacca atccacagtt tccccacaac tggcaacttg tgctttctca
      481 cccacctgct tatttgtacc ttgcggctat gcaagaatat ctcaacctgc tacggcatat
      541 tatagatacc ggtacgcata aaactgaccg gacgggtacc ggtacacaaa gtgtattcgg
      601 ctaccagatg cggtttgacc tgcaaaaagg gtttcccctg gtgactacaa aaaaagttca
      661 tcttaaaagt atcatatatg agcttctctg gttcttaaaa ggtgaaacca atatcgccta
      721 cctgaaagag catggtgtaa agatctggga tgaatgggca gatgagaaag gtgaactcgg
      781 gcctgtgtat ggcaaacaat ggagaagttg ggagggtaaa gatggaaaaa ttgtagacca
      841 gatatcagaa ttgattaccc agataaaaaa gaaccccgac agccgccgtt tgattgtcag
      901 tgcgtggaat gtggctgatt tgcctgagat ggccctgatg ccctgtcata atatgttcca
      961 gttttatgtg gccaatggga aactgagctg tcaattgtat caacgtagtg cagatgtttt
     1021 tttaggcgtt ccgtttaata ttgcctctta tgcattattg accatgatga ttgcccaggt
     1081 ttgtgatctt gaacccggcg agtttgtgca tacgtttggg gatgtgcata tttacagtaa
     1141 tcataaggag caggtgaacc ttcaattgag caggaatccg tttcctttac cggttatgaa
     1201 gctgaacccg gatgtaaaag atattttctc ttttcaattt gaagacttca cactggaaaa
     1261 ttatcaaagc catccgccta taaaggctcc ggtggcagta tagaactatt ggtatcttta
     1321 cacaatacta aagactcctg attaatgact atttctctgg ttgtggctgc agccgctaat
     1381 aatgcaattg gaaaagatgg aataatgccc tggcatttac ccaatgatat gaagcatttt
     1441 aaaaatgtta cctggggtat gccggtagtg atgggcagaa aaacgtttga ttcgctgggt
     1501 aaagcattac ccggcaggaa aaatattgtt attacccgtc agtcaggctg gaaggcagca
     1561 gcaactgtgg tagtaaagag tattgatgat gccttgtttg ttgccaaaga agctgatgca
     1621 aatgaagtaa tgataattgg tggaggggaa atatacaggg ccctttttga taaagctaaa
     1681 aggatatatc tcacccgtgt ggaagcagaa cctgaggccg ataccttttt cccatcgctt
     1741 gaccaggaac actggcatct ggttagtcag aaaaagcatg aggccgatgg gaaaaatgcc
     1801 tataattatt cttttgagat ttgggaaagg at
//
LOCUS       DAORTI010000297         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_106216_length_1821_cov_5.693658, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000297 DAORTI010000000
VERSION     DAORTI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106216_length_1821_cov_5.693658"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLO70_17465"
     CDS             <1..629
                     /locus_tag="PLO70_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQZ76311.1"
                     /translation="LMGLGWLLSQKGKSVFTISGKGFDLASIVMILGGLFLIYKSVKE
                     IHDKLEGEDPNEKSKNANGISFGYAIGQIMLIDAVFSFDSIITAGGTAKYVEIMIAAV
                     VIAMTVMFLFSTGIAAFIHKHPTLKMLALSFLVMIGLSLIIEGWDSESAHQLKLKNYI
                     YFRMAFSFIVEVLNMTMRKRMTKKRVVELNAPIIKEEEEDEGYSDMAK"
     gene            complement(761..>1821)
                     /locus_tag="PLO70_17470"
     CDS             complement(761..>1821)
                     /locus_tag="PLO70_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQZ76312.1"
                     /translation="DTVLHTLSWLFESLDPVTRELVADPDDGFLNPNQQSPEGEGFVS
                     FSIRPQANRHHLQQVMNTAKIVFDENAPIFTNVWKNIVDIEKPSSQVLPLPVVLNDTT
                     FLLRWSGTDAHAGIMGYDIYAIINDTITRRVLRNTPTDSIRINGVFGNTYKFFSVASD
                     LVGNIEDLPGVPDAVITLSAPLPLTILSFTGSRQDNDAVLQWRTENEVNVSQFELQRS
                     TNGTDFVTAGTVTAGGSFYSFTDPAVFLTRKEIFYRLKSVDTDSRFKYSAILKLSRQE
                     NGLLTVFPNPVNDQLVIGGLKSRGIVQIFGADGKLIQQKTATTQTMTVSMGGYTSGIY
                     WLQYTSDGELQTQKIIKQ"
BASE COUNT          541 a          353 c          386 g          541 t
ORIGIN      
        1 tgttgatggg tcttggttgg ttactaagtc aaaaaggaaa atccgttttt acaatttcag
       61 gtaaaggatt tgaccttgca agtattgtaa tgatacttgg tggattattt cttatttata
      121 aatcggtgaa agaaatacat gataagttgg aaggcgaaga cccgaatgag aaaagtaaaa
      181 atgcaaacgg aatctcattt ggttatgcaa taggccagat tatgttgatt gacgctgtat
      241 tctctttcga cagtattatt acagcaggag gtactgccaa atatgtagaa atcatgattg
      301 ctgctgtagt aattgcgatg acagtaatgt ttttattcag cacaggcatt gcagctttta
      361 ttcataaaca tccgacctta aaaatgcttg cattgtcttt tcttgttatg attggtttga
      421 gtttaattat cgagggctgg gatagtgaat cagcccatca gcttaaactg aaaaattata
      481 tttattttag aatggcattc tcctttatag tagaggtgct aaatatgacg atgcggaaga
      541 ggatgactaa gaaaagagtg gtggaattga atgcaccaat tataaaagag gaagaagagg
      601 atgaaggata ttcagatatg gcaaaataaa accgggtgcc tgacttagca gcacacccgg
      661 ccaattgagc atgaaaaaat ttactggtaa tttgcagatc tagccttata tgaaaatctt
      721 atcgccttat ttattggtat ctggttttat aactttcgga ttattgcttg ataatttttt
      781 gtgtttgcaa ttccccatca ctggtatatt gcagccagta tatgccactc gtataacctc
      841 ccatactcac ggtcattgtt tgagttgttg ctgtcttttg ttgaataagt ttgccgtcag
      901 ctccaaatat ctggacaata cctctacttt ttaatccccc aataaccaat tggtcattca
      961 ccggattagg aaaaaccgtt aataacccat tttcctgccg gctcaatttc agaatggcag
     1021 agtatttaaa ccggctgtct gtatctacag acttcaagcg ataaaaaatt tctttccggg
     1081 taaggaaaac tgccggatct gtaaacgaat agaaggaacc ccctgcagta actgttccag
     1141 ccgttacgaa atccgttcca ttagtactgc gctgcaattc gaattgactg acatttacct
     1201 cattttcagt ccgccactgc aatactgcat cattgtcctg ccggctaccg gtaaatgaaa
     1261 gaatggtcaa tggcagagga gcggaaaggg tgatcacagc atcaggcaca cctggcagat
     1321 cttcaatatt acctacgagg tcgctggcca cagaaaagaa tttgtaggta ttaccaaaaa
     1381 ccccgttaat acggatagaa tcagtaggtg tattgcgtaa tacccgacgg gtaatggtat
     1441 cgttaataat tgcatagata tcataaccca tgattcccgc atgcgcatca gttccgctcc
     1501 atcttaaaag gaatgtggta tcattcagca caactggaag gggtaacacc tggctggatg
     1561 gtttttctat gtccactata tttttccaga catttgtgaa aatgggtgca ttctcatcaa
     1621 aaactatctt ggctgtgttc atcacctgct gcaggtgatg ccggttcgct tgcggacgga
     1681 tactaaatga aacaaaaccc tctccttccg gtgattgctg gtttgggttc agaaacccgt
     1741 catccggatc ggcaaccagc tccctagtca caggatcaag cgattcaaag agccaggata
     1801 aagtatgaag taccgtatcc a
//
LOCUS       DAORTI010000298         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_106551_length_1818_cov_2.884288, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000298 DAORTI010000000
VERSION     DAORTI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106551_length_1818_cov_2.884288"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1323)
                     /locus_tag="PLO70_17475"
     CDS             complement(163..1323)
                     /locus_tag="PLO70_17475"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HQZ76313.1"
                     /translation="MKQNYALAFALTSLFFAVFCTSCSKNDGGTPSPNNPELPTVSTT
                     AATNITATTAESGGNVSSEGSSPVTARGVCWNQVAVPSTAGPHTVDGSGTGTYTSSVT
                     GLNPNTAYSVKAYATNSVGTAYGNSVLVITPQQLSIPTVTTIVPYNIKSYSVYTGGEV
                     TASGNATVTAKGVCWSTSPNPTIANNVLPYGSGGLGSFTSYVNTLTVHTTYYLRAYAT
                     NNVGTGYGEEYTLRTIYTIGEGLGGGKIFYLNQTNDHGLIAAIYDQSGGSKWNFTSNP
                     YSNINAYSLNNGMANTNAIIAVIGNAGHAAAVCRAYTGGGYNDWYLPSIDELNLLSQQ
                     KYIIGNFQSYYYWSSTASSNYVANAWSVDFGGGSTIEWDKTYIFYVRAIRAF"
     gene            1495..1725
                     /locus_tag="PLO70_17480"
     CDS             1495..1725
                     /locus_tag="PLO70_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQZ76314.1"
                     /translation="MTERDKKRLKKFGTHLRKLRKKKGLSIRELSSRCDVDYGKISKL
                     ENNKANLTVTTLIELAEGLDLHPKTLLDIDFE"
BASE COUNT          528 a          368 c          348 g          574 t
ORIGIN      
        1 atatagcttc gctttgtttt aatcttttca tttcttcatc acttccgcta ttgtttttcc
       61 aatatccgca gggattgcaa ccgaatgaat acgggattgg ttaaacatat acctttactt
      121 aaaattttac atgaatattt attaccaact tctcacctgt atttaaaatg cccgtatagc
      181 tcgaacataa aatatgtatg ttttatccca ttcaatagtg ctaccaccac caaaatctac
      241 agaccaggca ttcgcaacat aattgctgct cgcagttgag ctccagtaat aataactctg
      301 gaagtttcct atgatatatt tctgctgaga caagagattc aattcatcaa tggaaggcaa
      361 gtaccaatcg ttatatccgc ctcccgtgta tgcccggcat actgctgctg catgtcctgc
      421 gtttccaata actgcaataa ttgcattagt gtttgccatt ccattgttca aactatatgc
      481 attaatattg ctataggggt tagatgtaaa gttccatttt gatccgccgc tttgatcata
      541 aatagcagca atcaatccat gatcattcgt ttgattgaga tagaatattt tgccaccgcc
      601 aagaccttct ccaatggtat aaatagtccg aagtgtatat tcctcgccat agccagtacc
      661 cacgttattg gttgcatatg cacggagata gtaggtagta tggacggtga gcgtattaac
      721 ataactggtg aaacttccta atcccccaga gccatacgga agcacattat tggcaattgt
      781 cggatttgga gaggtactcc aacacacacc ttttgccgtt acagttgcat tgccagatgc
      841 agtaacctct cctccggtat aaacagaata actcttgata ttataaggta caatggtggt
      901 aacggttggg atactcaatt gctgtggcgt aatgacaagt actgaatttc cataggcggt
      961 tcctacactg tttgttgcgt aagctttaac tgaatatgcg gtattcggat ttaacccggt
     1021 aactgaactg gtgtaggttc ccgtacctga tccatcaacg gtatgtggac cagccgttga
     1081 aggtacagca acttggttcc aacacacgcc tcgtgcagta acagggctgc tcccttcgga
     1141 gctaacgttc cctccgcttt ctgctgtcgt tgcagtgata ttggttgcgg ctgtagttga
     1201 tactgttggc aactctggat tatttggaga tggagtacct ccatcatttt ttgagcaact
     1261 ggtgcagaaa actgcaaaga ataatgaagt cagggcaaat gccaacgcat agttctgttt
     1321 cataaaaata tgtttataat ataagattaa tagaaaatgt ccttttgaat cgcttttact
     1381 gcgtatcact tttctcaaag gatgtggttt gtatttcaaa tatataaatt gttggtagaa
     1441 ctaccaacaa tattttttca aatttccttg accttgtgtt gcagatatta catcatgacg
     1501 gaaagggata aaaaaaggct caagaaattt ggcactcacc tcagaaaact aagaaagaag
     1561 aaagggttga gtataagaga attatcctct cgatgtgatg ttgattatgg aaaaatcagc
     1621 aagctggaaa acaataaagc aaacttaacc gttacaacac ttattgagct ggctgaaggt
     1681 ctggatttgc atccaaaaac attactcgat attgattttg aatagtacac ttaattaaac
     1741 aatcccggtt cagtattttc taacattgct tctttatttc tcgtttgaat ctcttgctct
     1801 acagcccact ttggtttg
//
LOCUS       DAORTI010000299         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_107674_length_1807_cov_6.835046, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000299 DAORTI010000000
VERSION     DAORTI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107674_length_1807_cov_6.835046"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..636)
                     /locus_tag="PLO70_17485"
     CDS             complement(46..636)
                     /locus_tag="PLO70_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HQZ76315.1"
                     /translation="MFYEFKGIKPVADTTSFVHPQAAVTGNVIIGKHCYIGPGAALRG
                     DWGQIILEDGCNVQENCTIHMFPGVTVVLKEGAHIGHGAIIHGATIGKNCLVGMNSVI
                     MDNVLLGDESIVGALTLIKEGEIIPPRSVVVGNPGKIIKQVSDEMLNWKTEGTKLYQQ
                     LPENCYNSLKPCKPKRKVSSKQTAGNDSYSPWKTGI"
     gene            complement(666..1097)
                     /gene="paaI"
                     /locus_tag="PLO70_17490"
     CDS             complement(666..1097)
                     /gene="paaI"
                     /locus_tag="PLO70_17490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HQZ76316.1"
                     /translation="MNAEKDKLANDVVNHMMKHDLFSQWLGIEILEIKEGYSKIKMII
                     RKEMVNGFGIIHGGIAFSLADSAFAFACNNRNVLSVALDTSINFIKPVHVDDVLIAEA
                     TELHNGKSTGLYHINITNQKEHLIAQFKGLCYRTDKSLISQ"
     gene            complement(1109..>1807)
                     /locus_tag="PLO70_17495"
     CDS             complement(1109..>1807)
                     /locus_tag="PLO70_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQZ76317.1"
                     /translation="FNREMALLLQSTLDECKSEEVRCVYITGAGKAFCAGQDLAEVVD
                     PNGPGMQRILSEHYNPIVTRIRELEKPVVAAVNGVAAGAGANLAICCDITVAAKSASF
                     IQAFSKIGLVPDTGGSFFLPRLIGWQKAMALMMLGDKVPAEEAERMGMIYKYFEDGNF
                     TEESSKLAATLAAMPTKALYLIKKELQWSSTHTVYEQLQNEDKLQQRAAQTDDYKEGV
                     AAFLEKRQPVFKGK"
BASE COUNT          475 a          411 c          324 g          597 t
ORIGIN      
        1 ttcttttttt tatacaaagc atcagaaaag caaatagtat ttctcctata tacctgtttt
       61 ccagggcgaa tacgaatcat taccggctgt ttgttttgat gaaactttcc gcttcggttt
      121 acagggtttc aaactgttat aacaattttc aggcaattgt tgatataatt tggtcccttc
      181 tgttttccag tttagcattt cgtcactaac ctgtttgatg atcttgccgg gattcccaac
      241 caccacactt ctgggaggaa ttatttctcc ctcttttatt aatgtcagtg cccccactat
      301 actttcatca cctaatagta cgttatccat tatcacacta ttcataccta ccaggcaatt
      361 tttaccgatt gtagcaccat gaatgatagc gccatgtccg atatgggcgc cttcttttaa
      421 aacgacagta acaccgggaa acatatgaat ggtacagttt tcctgcacat tacaaccatc
      481 ttcaagaatg atctgccccc aatctcctcg taaggcagca ccggggccga tataacaatg
      541 tttgccaatg atgacattgc ctgttacagc agcctgcgga tgcacaaaag aggttgtgtc
      601 agcaacaggt tttatgcctt tgaattcata gaacatagta ctaagattta tggcgttaac
      661 atggattatt gtgagatcag ggatttatcc gttctgtagc aaaggccttt aaactgggca
      721 atcagatgtt ctttttgatt ggtgatattg atatgataca gcccggttga tttaccgtta
      781 tgcagttctg tagcttcggc aattaataca tcatccacat gtaccggttt tataaaattg
      841 atcgaagtat ccaatgcgac tgataaaaca ttgcggttat tgcaggcaaa ggcaaaagca
      901 ctgtctgcta atgagaatgc tatgccgcca tgaatgatac caaagccatt gaccatttct
      961 ttgcggatga tcatttttat tttgctatac ccctctttta tttccaatat ttctataccc
     1021 agccactggc tgaacagatc atgcttcatc atatgattga caacatcatt ggccaactta
     1081 tctttttcag cattcatttt ttatattttt attttccttt gaaaacaggt tgtctttttt
     1141 ctaaaaatgc agccactcct tctttgtagt catcagtttg tgcagctctt tgctgcagtt
     1201 tatcctcatt ctgtaattgc tcatacaccg tgtgagtgga agaccattgt aattcttttt
     1261 ttatcaggta caatgctttg gtaggcatcg cagccagtgt ggcagccagt tttgatgact
     1321 cttctgtaaa attcccgtcc tcaaaatact tatagatcat acccatacgt tcggcttctt
     1381 ctgccggtac tttatcgcct agcatcatca gtgccatagc tttttgccag ccaatcaatc
     1441 tgggtaaaaa gaaactgccg cctgtatctg gaactaatcc aattttagaa aatgcctgta
     1501 tgaaggatgc tgatttggca gctactgtaa tatcacaaca aatagcgaga ttagcaccgg
     1561 caccagctgc tacaccattt acagctgcta ctaccggttt ttccaattcc ctgatcctgg
     1621 taacaatagg attgtaatgc tcactcagta ttctttgcat tccaggtccg ttggggtcca
     1681 ccacttctgc aaggtcctgc ccggcacaaa aagcttttcc ggcacctgta atatatacac
     1741 atcgtacttc ttcgcttttg cattcatcca gcgttgattg aagcagtaaa gccatttccc
     1801 ggttaaa
//
LOCUS       DAORTI010000300         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_123596_length_1668_cov_6.831990, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000300 DAORTI010000000
VERSION     DAORTI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123596_length_1668_cov_6.831990"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /gene="asnB"
                     /locus_tag="PLO70_17500"
     CDS             <1..1502
                     /gene="asnB"
                     /locus_tag="PLO70_17500"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQZ76318.1"
                     /translation="NHFDGMFAFAIWDEKNRVLFAARDRFGEKPFFYFFNGQDFLFAS
                     EMKALWAAGVAKIPNQKMLFNFITIGYVDNPARPEETFYENIYKLPPACRLKYSGIYL
                     NYSVVQYWDIDLTAKQKNSSDADVIEQFIHLFSQSVKKRLRSDVPVGSSLSGGLDSSS
                     IAAISYEMQNSLQSHKCFTASFANFEKDETVFSKQVAAQFNLEQYTTAIAGNELLEDW
                     ETLCYHQEEPFGSASIFAQYKVFELAGQHNIKVLLDGQGADETLAGYHKYYKWYWQEL
                     FRKRKLYKSKELDAAHAIGINENFDYKNMIAAYFPVFASVVMERQYLLKAIRQEDLDK
                     EFVQLQSKEAYYTPPEHFDLNGVLYFNTCTHGLEELLRYADRNAMAHGREVRLPFLSH
                     ELVEFIFSLPSHFKIRHGWTKWLLREAMKDKLPAPIVWRKDKIGFEPPQKLWLEQPAM
                     QEAIQEAKRKLVNEKVLKKEVLNKKITALAAHDTDNYDWRYFSAASIFK"
     gene            1545..>1668
                     /locus_tag="PLO70_17505"
     CDS             1545..>1668
                     /locus_tag="PLO70_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQZ76319.1"
                     /translation="MKLATKLIHAGATPDPSTGAIMTPIFQTSTYVQEAPGVNKG"
BASE COUNT          512 a          336 c          345 g          475 t
ORIGIN      
        1 taaatcattt tgatggcatg tttgcttttg ccatctggga tgaaaagaac agggtattat
       61 ttgcagcaag agatcgtttt ggagaaaaac cattttttta ttttttcaac ggccaggatt
      121 ttttgtttgc ctctgaaatg aaagccttgt gggcagctgg tgtagcaaag attcccaatc
      181 agaaaatgct gttcaatttc attactattg gttatgttga taatcctgcc cgtcccgaag
      241 aaacttttta cgaaaatatt tataagctcc cccctgcctg ccggttaaaa tactcgggta
      301 tctatctgaa ctattccgtt gtgcaatact gggacattga ccttactgca aagcaaaaaa
      361 atagttcaga tgcagatgta attgaacagt tcattcatct tttctcacaa tctgttaaaa
      421 aaagattacg gagcgatgta cctgtaggct catcactgag tggcgggctt gatagttctt
      481 ccatcgctgc aataagttat gaaatgcaga acagcctgca aagccacaaa tgttttaccg
      541 cctcttttgc aaattttgaa aaagatgaaa cagttttttc aaaacaagtt gctgctcagt
      601 ttaacctgga gcaatacaca acagctatcg ccggaaacga attactggaa gactgggaaa
      661 ccctttgcta ccaccaggaa gaaccttttg gctctgccag tatttttgca cagtataaag
      721 tattcgaact ggccgggcag cataatataa aggttttact ggacggacag ggggctgatg
      781 aaacccttgc cggttatcat aaatactata aatggtactg gcaggaactt ttccggaaga
      841 ggaaattata taagagcaaa gagctggatg cagcccatgc aatagggatc aatgaaaatt
      901 tcgattataa aaatatgatc gctgcttatt tcccggtatt tgccagtgtg gtaatggagc
      961 ggcaatattt attaaaagct atccggcagg aagatctcga taaagaattt gtacaactgc
     1021 aaagcaaaga agcgtattat accccacccg agcatttcga tctgaacgga gttttatatt
     1081 tcaatacctg tacacatggc ctggaggaac ttttacggta cgctgacaga aatgctatgg
     1141 cacatggaag agaggtccgg ttgccttttt tatcgcatga actggtagag ttcatcttct
     1201 ccctcccttc ccattttaaa atcaggcacg ggtggaccaa atggctgcta cgggaagcta
     1261 tgaaagataa actacctgct cctattgttt ggcgaaagga taaaatcggt tttgagcccc
     1321 cgcaaaaact atggctggag caacctgcta tgcaggaagc tattcaggaa gcaaaaagaa
     1381 aattagtaaa cgaaaaagta cttaaaaaag aagtattgaa taaaaaaata actgctctcg
     1441 ctgcccatga taccgataat tacgattggc ggtatttttc cgcggcatct attttcaagt
     1501 aatcttattt cagcatcttt gcattctcaa ttaattacct cattatgaag cttgctacaa
     1561 aattaatcca tgccggtgca acacctgatc cttccactgg tgcgatcatg actcctattt
     1621 tccagacttc cacctatgta caggaagcac cgggagtaaa caagggtt
//
LOCUS       DAORTI010000301         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_134710_length_1587_cov_2.966710, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000301 DAORTI010000000
VERSION     DAORTI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134710_length_1587_cov_2.966710"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLO70_17510"
     CDS             <1..377
                     /locus_tag="PLO70_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76320.1"
                     /translation="FYSKVNISGTKYCFLEKDYRSGKLEYLANIESDEELPMSVFDKL
                     YTEWKAKIASLDFNGVPLVPYTNTKYKNGGEYEMYVKGDAWRLKTEGLSIDKKYFNFT
                     IRLEYLDLDQGGLMVRILITDN"
     gene            complement(418..>1587)
                     /locus_tag="PLO70_17515"
     CDS             complement(418..>1587)
                     /locus_tag="PLO70_17515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HQZ76321.1"
                     /translation="RNTDRTWGIGFKIMHLQGRSFENYINSLSAGLYTALATGYGLSN
                     KANGTPERNPYGNYLLGGFEFGLYISGSMGPGSGLEKLTNSWNLYLNIFQKTTQANFV
                     ADGILIYDFRQPLDTDGHKLTKEVIKSFQQTVVFKEATVPKPVTTTVTKKKPVTTTPV
                     VKKPVVPKPPVKTVPITNTGKPVKKVLTGKFTDQREGGGTYHTVKIGTQTWMSENLDV
                     STFLNGDQIPEAKTVEEWKSAGANRKPAWCYYNNNDENGKLYNWFAVNDPRGLAPKGW
                     HVSTRDDWVKMITYLGGDDIAPNKLIDGPGWKRNIAGNLESGFAGLPGKWRTGEGIFT
                     DTFESGSWWTSSYKNYIDAATLYTLYYADGISSMDLSGQGSDKKLGASVRCVKNQ"
BASE COUNT          434 a          352 c          315 g          486 t
ORIGIN      
        1 aattttattc aaaagtgaat atcagcggaa ctaaatattg cttcctggaa aaagactata
       61 ggtcgggaaa actggagtat ctcgctaaca tagaatcaga tgaagaactg cccatgtctg
      121 tttttgataa attatatacg gaatggaaag ccaaaattgc ttcgcttgat tttaatggtg
      181 taccactggt gccttataca aatactaaat acaaaaacgg tggtgagtat gaaatgtatg
      241 tgaaaggcga cgcatggcgg cttaaaacag aaggcttgtc gattgataaa aaatatttca
      301 actttaccat caggctggag tacctggatc tggaccaggg aggtttgatg gtgagaatac
      361 ttattacaga taactaaacc tgcccctttc acttccgccc atggcattct attatactta
      421 ttgattcttc acgcaacgaa ctgatgcacc caacttttta tctgagcctt gcccactcaa
      481 atccattgaa gagataccgt cggcataata gagggtgtac agtgttgctg catcaatata
      541 atttttataa gaagaagtcc accaactgcc ggactcaaaa gtatcagtaa aaataccttc
      601 tccggtacgc catttgcccg gcagcccggc aaaaccgctt tcgaggttgc ctgcaatatt
      661 tcttttccat ccaggcccgt ctatcaattt attgggagct atatcatctc ctcccaggta
      721 cgtaatcatt ttgacccagt catcccgggt ggatacatgc cagcctttcg gcgcaagtcc
      781 acggggatca ttaaccgcaa accaattata aagctttcca ttttcatcat tattgttgta
      841 ataacaccag gcgggttttc gatttgcgcc ggcacttttc cattcttcca cagtcttcgc
      901 ttcaggtatc tggtctccat tcagaaaagt gctaacatcc aggttctctg acatccaggt
      961 ttgggtgcca atttttacag tatgataagt gcctcccccc tctctttgat cggtgaattt
     1021 tcctgtcagt acttttttaa caggcttacc tgtgttagta attgggaccg tttttacggg
     1081 tggttttggt acaacaggtt tctttacaac cggagtggtt gtaaccggct tctttttggt
     1141 aacagttgtt gtcactggtt ttggaacggt ggcctcttta aatacaaccg tttgctgaaa
     1201 agatttaatg acttctttag tcagcttatg accatctgta tccaaaggct gccggaaatc
     1261 ataaattaag atgccatctg ccacaaaatt ggcctgggtg gttttttgga aaatatttaa
     1321 atataaattc cagctgttgg ttaatttttc taaccccgag ccaggcccca tggaaccact
     1381 gatatacagt ccaaattcga atccgccaag caggtaattt ccgtaagggt tcctttcagg
     1441 agttccgtta gccttgttcg ataatccata tccggtggcc aatgccgtat aaagaccggc
     1501 ggaaagacta ttaatataat tttcaaaact tcttccctgc aggtgcatta tcttaaaacc
     1561 aattccccat gttctatcag tattccg
//
LOCUS       DAORTI010000302         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_137655_length_1567_cov_6.788360, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000302 DAORTI010000000
VERSION     DAORTI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137655_length_1567_cov_6.788360"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLO70_17520"
     CDS             complement(<1..196)
                     /locus_tag="PLO70_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQZ76322.1"
                     /translation="MQKTLPTWNLIYQGLLSNIPVMLLYVLESKGSSPGRQGFFMAVN
                     ANGEMEGSVGGGIMEHKFVEM"
     gene            complement(207..>1567)
                     /locus_tag="PLO70_17525"
     CDS             complement(207..>1567)
                     /locus_tag="PLO70_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQZ76323.1"
                     /translation="ERTLFHCNNTYFIPNVKATAYSCRTNLPPNTAFRGFGGPQGMFV
                     IEAAIAKAAEALNISASVIQQKNLLKTGDEFPYGQIAASEANECWNKADEQFNIKELQ
                     KEIDDFNASNKNYKKGLALMPICFGISFTKTLMNQARSLVHVYTDGSVAVSTGAVEMG
                     QGVNTKMLQVAASIFSINPSKIKINTTNTYRIANTSPTAASATADLNGKATEIACATI
                     LERLKSVAAEELNVAVASIEIKDEFIFAAGKKTDWDWKRLVMTAYVKRINLSEHAHYT
                     PPAIHFDATIEKGHPFAYHVYGTSITTVTVDCIRGIYEVDAVKVVHDFGKSMNTMIDI
                     GQCEGGIVQGIGWMTMEEIVYDKEGKLRSNALSTYKVPDIYSVPKEITIHPLSTEKDN
                     LAIFRSKAVGEPPLMYGIGAYFALYNAVKAFNPTTDISFDAPMTPEKVLMSLYPQPLK
                     GS"
BASE COUNT          441 a          342 c          268 g          516 t
ORIGIN      
        1 ccatttccac aaacttatgt tccatgattc caccaccgac agaaccttcc atttcaccat
       61 ttgcattcac cgccataaaa aatccctgac ggcccgggct gcttccctta ctttccaaca
      121 catataaaag cattacaggt atattactca ataaaccttg ataaattaag ttccatgtcg
      181 gtaaagtttt ttgcatagaa agttttctaa ctccccttta ggggctgggg gtataaactc
      241 atcaatactt tctctggcgt catcggcgca tcaaaagaaa tatcagttgt aggattaaaa
      301 gccttcactg cattatacaa agcaaagtaa gcaccaatgc catacatcag cggcggttcg
      361 ccaacggcct tacttctgaa aatagccagg ttatcttttt cggtagataa aggatgtata
      421 gttatttctt ttggtacaga ataaatatca ggcactttgt aagtgctcaa tgcgttgctg
      481 cgaagttttc cttctttatc atatacaatt tcttccatcg tcatccagcc gatgccttgt
      541 acgatgccgc cttcgcattg tccgatgtca atcatggtgt tcatactttt tccaaaatca
      601 tgcaccacct ttactgcatc cacttcatag ataccacgga tgcaatcaac agtaacagtt
      661 gtaatggaag tgccatacac atgatacgca aaaggatggc ctttttctat tgttgcatca
      721 aaatgaattg ctggtggtgt ataatgtgca tgttcggata ggttgatacg ttttacataa
      781 gcggtcatca ctaatctttt ccaatcccaa tccgtttttt taccggcggc aaaaataaat
      841 tcatctttta tttctattga tgcgacagca acatttaatt cttcagcagc aacgcttttc
      901 aatctttcta aaattgtagc gcaggcaatc tcagtggctt tgccattcag atctgcagta
      961 gcactggcag ccgtaggaga tgtattggca atacgataag tgttggttgt gtttattttt
     1021 attttggaag gattaataga aaaaatactg gctgctacct gaagcatttt tgtatttact
     1081 ccttgcccca tttctactgc gccagtactt acagccacac taccatctgt ataaacatgt
     1141 accaacgatc ttgcctgatt catcaatgtt ttagtaaaag agataccaaa acaaattggc
     1201 attaatgcta accctttttt ataattttta ttcgacgcat taaaatcatc aatctctttt
     1261 tgtagttctt tgatattaaa ttgttcatcc gctttgttcc agcattcgtt cgcttcacta
     1321 gctgcaattt gtccgtaagg aaattcgtca ccggtcttca ataaattctt ttgctgaatt
     1381 acacttgcag aaatatttaa tgcttctgct gcttttgcaa tagctgcttc tattacaaac
     1441 attccctgag gaccgccaaa tccacgaaag gcagtattgg gtggaaggtt tgtccggcaa
     1501 ctataagcgg ttgcttttac attgggaata aaatatgtat tgttacaatg aaacaacgtt
     1561 ctttcca
//
LOCUS       DAORTI010000303         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_137705_length_1567_cov_3.058862, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000303 DAORTI010000000
VERSION     DAORTI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137705_length_1567_cov_3.058862"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1567)
                     /locus_tag="PLO70_17530"
     CDS             complement(<1..>1567)
                     /locus_tag="PLO70_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQZ76324.1"
                     /translation="SYNDSEAKAEQILKAVPKDGKYDAIIVGIHNFSNRPAGNYGISP
                     AAINLWKELNFIKTETIVFGNVLATKNFCDAWTLVACYQDDDITQQVAADYLKGKFEA
                     IGTLPVRVCRFNSGDGIVKAKTTPVHQYSKLNEIDSIVADGIAKKAFPGCVVLAVHDG
                     NIIYHKAFGNYKYETSPATTFESIFDLASVTKISATTVSVMKLYEEGKLDIKKTLGDY
                     LPLVKGTDKADLKLDDIILHQAGLVPYIAFYKETIDPKTGKPNPAIFSEKSKPGFTVR
                     VAENIFMRNDWQDTMFSRILNSKLSAHGKYVYSDNDFIFLGKIVEQISGMPLNEYAAQ
                     TFYRPIGMTTTGFKPRERFAVNKMVPTETENHFRMQTTQGDVHDEGASMFGGVSGHAG
                     LFSDAYDLAMLYQMLLNGGIFNGKRYLKKETIDLFTAYHSDVSRRGYGFDKPEKDNAL
                     RKEPYPSALCSPATFGHTGFTGTCVWVDPKASLVYVFLSNRVYPTRDGNLLGRMNIRS
                     KIQDTIYRALGEGE"
BASE COUNT          450 a          334 c          302 g          481 t
ORIGIN      
        1 attctccttc acccaatgca cgataaatgg tgtcctgtat tttgctgcgt atgttcatac
       61 ggcccagcag attaccatct cttgtagggt acactctgtt tgaaaggaat acataaacca
      121 gacttgcttt tggatctacc cacacgcagg tgccggtaaa accggtatgt ccaaaagttg
      181 cgggtgagca aagtgcagaa gggtaaggtt cttttcgcag tgcattatct ttttcaggtt
      241 tatcaaatcc ataaccacgc ctgcttacat cgctatgata ggctgtaaaa agatctatcg
      301 tttctttttt caaataacgt ttgccattaa aaataccgcc gttgagcagc atttggtaca
      361 gcatggccag atcgtaagca tcggagaata aaccggcatg gcctgaaaca ccgccaaaca
      421 tggaagcgcc ttcgtcatgc acatcgccct gagtggtttg catacgaaag tgattttcag
      481 tttcggtagg caccatttta ttcactgcaa atctttcacg aggcttgaag ccggttgtgg
      541 tcatgccgat aggccggtaa aaagtttgag cagcatattc attgagtggc atgccggaga
      601 tttgctcaac aatttttccc agaaaaataa aatcgttatc gctgtacaca tatttgccat
      661 gggcagaaag tttgctgttg agtattcgac tgaacatagt atcctgccag tcgttgcgca
      721 taaaaatatt ttctgctaca cgtactgtga aaccgggttt ggatttttca ctgaaaatgg
      781 ctggattggg tttcccggtt ttaggatcaa tggtttcttt ataaaaagca atataaggaa
      841 ccagccctgc ctgatgcaga ataatatcat ccagttttaa atctgcctta tctgttccct
      901 ttaccaaggg caaataatcg cctaaagttt ttttaatatc caatttacct tcttcataca
      961 acttcattac cgaaacggta gtggcagaaa ttttggttac agaagccaaa tcaaaaatgc
     1021 tttcaaatgt agtagccgga gaagtttcat atttataatt tccaaatgct ttatgataaa
     1081 taatatttcc atcatgcaca gccaatacca cacagccggg aaaagctttt ttggcaatgc
     1141 catcggcaac aatagaatca atctcattta gtttgctgta ctgatgtact ggtgtcgtct
     1201 ttgcttttac tataccatca ccgctattga atctgcacac acgaaccgga agcgttccga
     1261 tagcctcaaa ttttcctttc aaataatcag cagccacttg ctgagttatg tcatcatcct
     1321 gataacaggc taccagtgtc catgcatcac aaaagttttt agtggccagt acatttccaa
     1381 aaacaatggt ttctgttttg atgaaattta attctttcca taaattaata gcagcaggag
     1441 aaataccata attacctgca ggccggttac tgaaattatg aatgcccact ataatggcat
     1501 catattttcc atctttggga acagccttaa gaatttgttc ggcttttgct tcactatcat
     1561 tgtagga
//
LOCUS       DAORTI010000304         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_140557_length_1548_cov_6.581380, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000304 DAORTI010000000
VERSION     DAORTI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140557_length_1548_cov_6.581380"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1548
                     /locus_tag="PLO70_17535"
     CDS             <1..>1548
                     /locus_tag="PLO70_17535"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HQZ76325.1"
                     /translation="FASAPDQVIVVRITADKPGAISFITNLRGERNQAHSNYGTDYFQ
                     MDPYGNDGLVLTGKSTDYLGVEGKMRYEARIKAVPEGGTMKTDGVDLVIENANAVTLY
                     FAAATNFVNYKDVSADQHRRVDNYFKGIEGKSFNAIAASAVADHQQYFNRVKLQLPNT
                     ANSFLPTPERVNKIQTEPDPSMAALSYQYGRYLLIGSSRPGTEPANLQGIWNDNMNPS
                     WDSKYTTNINTEMNYWPVESGNLSECAEPLVRMIKELTDQGSQVAKEHYGARGWVLHQ
                     NTDIWRVAAPMDGPTWGTFTVGGAWLCTHLWEHYQYTRDKEFLKDAYPLLEGAVQFFM
                     DFLVPHPNGKWLVTNPSTSPENFPDGGGNKPYFDEVTAGFREGTTICAGSSIDMQILY
                     DLFGYYTEAAKVLEKNDVFIDSVKTAREKLVPPQIGKDGSLQEWADDWKSLEKNHRHF
                     SHMYGLYPGKILYEKRTPALIESYKKVLEERGDASTGFSRAWKMALWARLADGNRANK
                     IYKGYLK"
BASE COUNT          479 a          304 c          374 g          391 t
ORIGIN      
        1 atttgcttcc gcacccgacc aggtaattgt agtacgcatt actgctgata agcccggcgc
       61 aatttccttt atcactaacc tgcggggaga aagaaaccag gcacattcta actacggtac
      121 agattatttc cagatggatc cttatggcaa tgatggatta gtacttacag gaaagtcaac
      181 agattatttg ggggtagaag gaaaaatgag atatgaggct agaataaaag ctgtgccgga
      241 agggggcacc atgaaaacag atggagttga tctggtaatt gaaaacgcca atgcagtgac
      301 tttgtatttt gctgctgcta ccaattttgt aaactataaa gatgtaagcg ccgatcaaca
      361 ccggcgggtg gataattatt tcaagggtat tgaaggcaaa tcattcaatg ctattgcagc
      421 gtccgctgta gcggatcatc agcaatattt taacagggta aaactgcaac tgcccaatac
      481 tgctaattct tttttaccaa cgccggaaag agttaataaa attcagaccg agcccgatcc
      541 ttccatggct gcactgagtt atcagtatgg ccgttatctc ctgattggtt cttcaagacc
      601 gggaacagaa cccgccaacc tgcagggtat ctggaatgat aatatgaatc cttcgtggga
      661 ttctaaatac accaccaaca tcaatacgga aatgaattac tggccggtgg aaagcggcaa
      721 tctttctgaa tgtgccgaac cattagtaag aatgattaaa gaattaaccg atcagggttc
      781 acaggtggca aaagaacatt atggtgcaag aggttgggta cttcatcaga atacagatat
      841 ctggagagtg gcggcaccca tggatggtcc tacctgggga accttcaccg tgggcggtgc
      901 ctggctctgc acacatttgt gggagcatta tcaatacaca agggataaag aatttttgaa
      961 agatgcttac ccgctacttg aaggcgctgt acaattcttt atggactttt tagtaccgca
     1021 tccgaatgga aaatggctgg tcaccaatcc gtccacatca cctgaaaact ttcccgatgg
     1081 cggcggcaat aaaccctact ttgatgaagt gactgcaggt ttcagggaag gcacaacgat
     1141 ttgcgccggt tcttctattg atatgcagat actctatgac ctctttggtt attatacaga
     1201 agcggctaaa gtgttagaaa aaaacgatgt ctttattgac agtgtaaaaa cagccaggga
     1261 aaaattagtg ccgccgcaaa taggaaaaga tggttcgttg caggaatggg cggatgactg
     1321 gaaatcgctg gaaaaaaatc accggcattt ttcacatatg tatggtttat acccggggaa
     1381 aattttatac gaaaaaagaa caccggcttt gattgagtcg tataaaaaag tattggaaga
     1441 aagaggtgac gcctccaccg gtttttcaag agcatggaag atggcgttgt gggcaagact
     1501 ggctgatgga aaccgggcga ataaaattta taaaggctac ctaaaaga
//
LOCUS       DAORTI010000305         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_5295
            NODE_144192_length_1526_cov_5.711081, whole genome shotgun
            sequence.
ACCESSION   DAORTI010000305 DAORTI010000000
VERSION     DAORTI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867512
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:23:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,474
            Genes (coding)                    :: 3,468
            CDSs (with protein)               :: 3,468
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144192_length_1526_cov_5.711081"
                     /isolate="AMR_MDS_5295"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLO70_17540"
     CDS             complement(<1..664)
                     /locus_tag="PLO70_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQZ76326.1"
                     /translation="MKWFFLCLLLLLVGIYIFIQTPFGQNWIARQVTSRLSKDLQTKI
                     SIKHIDFSLFNRMNLEGVLIEDRKRDTLLYAGNLKVNITDWFFFKKNIELKYIGLDNA
                     IIKFQRSDSIWRQQFIFDYFSSTTKDTAAKKKSGITLNLKIVELRNVIFVKKDAWLGE
                     DMNIKVGALRLDANNMAFSGNKYEINSLLIKDPVVALHSYTKLKPALATVIPGEEADT
                     TAT"
     gene            812..>1526
                     /locus_tag="PLO70_17545"
     CDS             812..>1526
                     /locus_tag="PLO70_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQZ76327.1"
                     /translation="MRKFFLIFSIFILNKSFAQVTPQKLSDKYVLVIHGGAGTIVKSQ
                     MSPERENAYKAALDKALQTGNAILKSGGTALDAVEATVRSMEDDPLFNAGKGAVFTNE
                     GKNELDAAIMNGKTLEAGSVAGVTIIRNPITAARAVMEESPHVMMTGAGAEKFAKQQG
                     LEIVDPSYFYTEERWKSLQKAKQQDSLKMHLDHADTTNRGMLKQWENKDYKYGTVGAV
                     ALDRYGNLAAATSTGGMTNK"
BASE COUNT          476 a          269 c          338 g          443 t
ORIGIN      
        1 aggttgccgt agtgtcagct tcttcgccgg gtattactgt agcaagagca ggtttaagtt
       61 ttgtataact gtgtaaggca actacaggat cttttattaa taatgaattg atctcatatt
      121 tattcccgga aaaagccatg ttattggcat cgagcctgag tgccccgact ttgatgttca
      181 tgtcttcacc taaccaggca tcttttttaa caaaaatcac atttcggagc tccactattt
      241 tcagattcaa tgttatcccg ctttttttct ttgctgctgt atcttttgta gtggaagaaa
      301 agtaatcgaa aatgaactgc tggcgccata tggaatcact gcgttgaaat tttataatgg
      361 cattatcaag cccgatatat ttcaactcta tatttttttt gaaaaagaac cagtcagtaa
      421 tatttacttt cagattgccg gcgtagagta aggtgtctcg tttgcggtct tcaatgagta
      481 caccttccag gttcattcgg ttaaataacg aaaaatcaat atgcttaata gaaatttttg
      541 tttgcaggtc tttggaaagc ctgctggtta cttgccgtgc gatccaattt tgtccgaagg
      601 gggtttgaat aaaaatatat atacccacta acaatagcag caggcataag aaaaaccact
      661 tcaatatttt tttaaatcgc ttcagactca gctatttagt gtaaaagtaa gtaatcgcct
      721 cattagggca aattcaatac caatattagg gacaaacata tacgtaagca gtgcaaacgg
      781 cagaatactt atattgcttt aaaaatttgc aatgagaaag ttttttctga ttttttcaat
      841 tttcattctt aacaaaagtt ttgcgcaggt cacacctcag aaattgagtg ataaatatgt
      901 acttgtcatt catgggggtg cgggaacaat agttaagagc cagatgtccc cggaaagaga
      961 gaatgcttat aaagcggccc ttgataaggc attacaaacg ggtaatgcta tactgaaaag
     1021 cgggggcact gcattagatg cagtggaagc caccgttcgg agcatggagg atgatcccct
     1081 tttcaatgca ggtaaaggtg ctgtttttac gaatgagggg aagaatgagc tggatgctgc
     1141 aataatgaat ggaaaaacat tggaagccgg atcggtcgct ggagtcacaa tcattcgtaa
     1201 tcctattact gctgcaagag cagtgatgga ggaaagtccc catgtgatga tgacgggagc
     1261 cggggctgaa aaatttgcta aacaacaggg gcttgaaatt gtggatccat cctatttcta
     1321 cacggaagaa aggtggaaaa gtttgcaaaa agctaagcaa caggattctt taaaaatgca
     1381 tttagatcat gcggatacaa ccaatagagg gatgcttaaa caatgggaga ataaagatta
     1441 taaatatgga accgttggtg ctgtcgcatt agaccgttac ggaaacctgg ctgctgctac
     1501 aagtaccggc ggaatgacga ataaaa
//
